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Sample records for radioimmunoassay ria radioreceptorassay

  1. Microcapillary blood sampling for serological examinations by radioimmunoassay (RIA) and enzyme immunoassay (ELISA)

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    Rodak, L.; Smid, B.; Valicek, L.; Jurak, E. (Vyzkumny Ustav Veterinarniho Lekarstvi, Brno-Medlanky (Czechoslovakia))

    1984-01-01

    Methods were tested of sampling blood and blood serum for serological examinations on filtration paper and into heparinized glass capillaries with transfer into the dilution solution of the given composition. Samples were also examined for ACH virus antibodies. The suitability of the sampling was verified by an examination of samples using ELISA and RIA methods. The results showed the suitability of sampling using microcapillaries. The titres of virus antibodies found using the ELISA and RIA methods were identical and the sensitivity of antibody detection was not reduced even after the sample had been stored for 60 days at a temperature of 20 degC.

  2. Non-radiometric immunoassays fluoroimmunoassay (FIA) and fluorometric enzyme immunoassay (FEIA) and radioimmunoassay (RIA) for evaluation of thyroid function in normal and hypothyroid dogs

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    Jerico, M.M.; Larsson, C.E. [Sao Paulo Univ., SP (Brazil). Faculdade de Medicina Veterinaria e Zootecnia. Dept. de Clinica Medica]. E-mail: marciajerico@hotmail.com; Mendonca, B.B. [Sao Paulo Univ., SP (Brazil). Faculdade de Medicina . Lab. de Hormonios e Genetica Molecular; Otsuka, M. [Sao Paulo Univ., SP (Brazil). Faculdade de Medicina Veterinaria e Zootecnia. Hospital Veterinario; Maganin Junior, A. [Canil da Policia Militar do Estado de Sao Paulo, SP (Brazil)

    2001-07-01

    We proposed the comparison of thyroxine (T4) and free thyroxine (FT4) measurements by fluoroimmunoassay (FIA) and fluorometric enzyme immunoassay (FEIA) with radioimmunoassay (RIA) in thyroid function evaluation of normal (n=50) and hypothyroid dogs (n=9). T4 and FT4 serum concentrations were measured in basal conditions and 6 h after TRH stimulation (200 mug/IV). All our reference values are based on the 5th and 95th percentile. The reference values for basal T4 in healthy dogs were 0.50 to 2.35 mug/dL (FIA), 0.50 to 2.51 mug/dL (FEIA) and 0.35 to 0.74 mug/dL (RIA). After TRH, the values were >= 1.37 mug/dL (FIA), >= 0,26 mug/dL (FEIA) and >= 0.40 mug/dL (RIA). Basal FT4 values in healthy dogs were 0.65 to 2.20 ng/dL (FIA), 0.38 to 1.43 ng/dL (FEIA) and 0.10 to 1.24 ng/dL (RIA). After TRH, the values were >= 1.30 ng/dL (FIA), >= 0.77 ng/dL (FEIA) and >=0.50 ng/dL (RIA). In hypothyroid dogs, the mean +- SD for T4 in basal conditions and after TRH were 0.24 +- 0.20 mug/dL and 0.26 +- 0.20 mug/dL (FIA), 0.27 +- 0.12 mug/dL and 0.32 +- 0.51 mug/dL (FEIA) and 0.19 +- 0.30 mug/dL and 0,24 +- 0.09 mug/dL (RIA), respectively. In the same group the mean +- SD basal FT4 values and after TRH were 0.28 +- 0.33 ng/dL and 0.28 +- 0.39 ng/dL (FIA), 0,12 +- 0.26 ng/dL and 0.23 +- 0.56 ng/dL (FEIA) and 0,15 +- 0,15 ng/dL and 0,17 +- 0,28 ng/dL (RIA), respectively. Significant differences (p<0.05) between the normal and hypothyroid groups (Kruskall-Wallis test) were observed in the three methods and between basal and stimulated values in normal dogs (Wilcoxon test), by the three methods. The best sensitivity for diagnosing hypothyroidism was obtained through T4 values (100%), and the best specificity through FT4 values (100%), both determined by FIA after TRH stimulation. We conclude that T4 and FT4 measured by fluoroimmunoassay after TRH stimulation can be an excellent alternative. (author)

  3. Radioimmunoassay for aclacinomycin A.

    Science.gov (United States)

    Matsuzawa, Y; Kiyosaki, T; Oki, T; Takeuchi, T; Umezawa, H

    1982-04-01

    An antibody against aclacinomycin A (ACM) was produced in a rabbit by immunization with ACM-bovine serum albumin conjugate. The radioimmunoassay (RIA) was based on the competition of unlabeled anthracycline with 3H-labeled ACM for binding sites on a specific antibody. Antibody-bound and free antigen were separated by selective adsorption on dextran-coated charcoal. The antibody reacted equally with ACM and its metabolites, MA144 M1, MA144 S1 and aklavin, and could precisely distinguish aklavinone-related aglycones, adriamycin and daunomycin. RIA was sensitive in the range of 1 approximately 10 pmol per assay. The quantities of ACM and its metabolites in human plasma were practically determined without any pretreatment of physiological samples.

  4. Prostatic acid phosphatase by radioimmunoassay

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    Lindholm, G.R.; Stirton, M.S.; Liedtke, R.J.; Batjer, J.D.

    1980-11-07

    Prostatic acid phosphatase values in 98 patients with prostatic carcinoma were measured by a commmercial radioimmunoassay (RIA) and by enzymatic assay. Forty-three carcinomas were staged by rigorous pathological criteria. Patients (N = 129) with benign prostatic hyperplasia were the control group. At 94% specificity, sensitivities of the RIA vs the enzymatic assay for clinically staged patients were as follows: stage A, 22% vs 6%; B, 29% vs 10%; C, 52% vs 38%; and D, 87% vs 80%. However, none of the seven patients with pathological stage A and B disease had a positive test result, and we suggest that variability in staging criteria accounts for the discrepant sensitivity claims reported. Prostatic acid phosphatase RIA should not be used for screening but as an adjunct for staging known prostatic carcinoma.

  5. Radioimmunoassay analysis of baculovirus granulins and polyhedrins

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    Summers, M.D.; Hoops, P.

    1980-05-01

    Granulin and polyhedrin proteins were purified by preparative sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis from the baculoviruses Autographa californica, Rachiplusia ou, Heliothis zea, Heliothis armigera. Trichoplusia ni, and Spodoptera frugiperda. Antisera were raised against Autographa californica (Ac) polyhedrin and Trichoplusia ni (Tn) granulin and analyzed for homologous and heterologous immunoreactivity by immunodiffusion and radioimmunoassay (RIA). Ac polyhedrin and Tn granulin antisera recognized antigenic determinants on several baculovirus polyhedrin and granulin proteins even though the heterologous proteins had different immunoreactivities when compared by competition radioimmunoassay. Antigenic differences among granulin and polyhedrin proteins were also detected by altered slopes of the competition reaction curves. Antiserum raised against Ac polyhedrin which was purified in the presence of SDS was tested by competition RIA for its ability to detect and react with native polyhedrin produced in the infected TN-368 cells. Ac polyhedrin antiserum had similar if not identical ability to bind to native polyhedrin and to polyhedrin purified in the presence of SDS.

  6. Examination of illegal, non declared injection preparations on anabolic hormones and development of a radioimmunoassay for 19-nortestosterone

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    Rapp, M.; Meyer, H.H.D.

    Procedures are described for the identification and quantification of anabolic hormones in preparations for injection. To perform radioreceptorassays for the most important groups of anabolics, estrogens and androgens, hydrolysis of esters is absolutely necessary in order to release the active substances from depot preparations. After moderate hydrolysis (30 min; 45/sup 0/C) it was possible to identify 19-nortestosterone-17..beta.. as active substance in three various illegal solutions for injection and one of them contained in addition estradiol-17..beta... After more drastic hydrolysis (2 h; 70/sup 0/C) there was a strong disintegration of 19-nortestosterone-17..beta.. and new more polar compounds were found, e.g. in small amounts 6-ketoestradiol, with estrogenic activity. Among the anabolic steroids only trenbolone-17..beta.. behaved in similar manner, and the disintegration during drastic hydrolytic conditions gave an indication for the existence of 19-norsteroids. Specific radioimmunoassays and GC/MS were used for final identification of the anabolic agents. Quantitation was carried out with HPLC/UV and radioimmunoassays after moderate hydrolysis. Since 19-nortestosterone-17..beta.. was identified a specific radioimmunoassay for this hormone was developed. Antibodies were raised against 19-nortestosterone-17..beta..-HS-BSA in order to be able to measure 19-nortestosterone-17..cap alpha.., 19-nortestosterone-17..beta.., and 19-norandrostenedione which were expected as the main bovine metabolites.

  7. Radioimmunoassay of thyroxine in saliva

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    Putz, Z.; Vanuga, A.; Veleminsky, J. (Institute of Clinical Endocrinology, Lubochna (Czechoslovakia))

    1985-04-01

    A simple radioimmunoassay (RIA) for thyroxine (T/sub 4/) in saliva has been described. Fifty euthyroid control subjects, 14 euthyroid pregnant women, 23 thyreotoxic and 10 hypothyroid patients were examined. Serum T/sub 3/, T/sub 4/, thyroxine binding globulin (TBG) and TSH were measured simultaneously. The mean level of T/sub 4/ in saliva in controls was 1.10 +- 0.07 nmol/l. There was a good correlation between the saliva and serum T/sub 4/ concentrations (r = 0.74) and between saliva T/sub 4/ values and the T/sub 4//TBG ratio (r = 0.83). The saliva T/sub 4/ levels, like serum free T/sub 4/, were not dependent on fluctuations of serum TBG concentrations. In euthyroid pregnant women, saliva T/sub 4/ levels were within the normal range while the serum T/sub 4/ and TBG were increased. There was a good agreement of saliva T/sub 4/ values with the functional state of the thyroid. Thus, the RIA of saliva T/sub 4/ could replace the laborious determination of serum free T/sub 4/. It can especially be useful in instances with abnormal values of TBG, as it is in pregnancy, in congenital deficiency of serum TBG or in subjects with hereditary elevated TBG levels.

  8. Measurement of Steroids in Rats after Exposure to an Endocrine Disruptor: Mass Spectrometry and Radioimmunoassay Demonstrate Similar Results

    Science.gov (United States)

    Commercially available radioimmunoassays (RIAs) are frequently used in toxicological studies to evaluate effects of endocrine disrupting chemicals (EDCs) on steroidogenesis in rats. Currently there are limited data comparing steroid concentrations in rats as measured by RIAs to t...

  9. Radioimmunoassay of buprenorphine

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    Bartlett, A.J.; Lloyd-Jones, J.G.; Rance, M.J.; Flockhart, I.R.; Dockray, G.J.; Bennett, M.R.D.

    1980-01-01

    Antisera to buprenorphine were obtained in rabbits immunised with 3-0-carboxymethylbuprenorphine and N-hemisuccinyl-norbuprenorphine conjugated to bovine serum albumin. Using the latter antiserum and tritium labelled buprenorphine a radioimmunoassay having good accuracy and precision was developed for concentrations as low as 50 picograms in 1 ml of plasma. The N-hemisuccinyl antiserum crossreacted with norbuprenorphine, and the 3-0-glucuronide conjugate with the 3-0-carboxy-methyl antiserum. Cross-reactivity of both antisera to other pharmacologically related compounds was negligible. The assay was employed to determine plasma buprenorphine concentration following its parenteral administration to dog and man.

  10. Radioimmunoassay for atriopeptigen

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    Munie, G.E.; Rowold, E. Jr.

    1986-03-01

    Atriopeptigen is a precursor which, when cleaved releases atriopeptin, an important peptide in natriuresis and diuresis. The authors describe a radioimmunoassay for atriopeptigen that permits quantitation of N-terminal atriopeptigen fragments following cleavage of atriopeptin. A New Zealand white rabbit was immunized with a thyroglobulin conjugate of a synthetic peptide of rate atriopeptigen (amino acids 72-91). High titer rabbit antibody (final titer of 12,000) was produced that is specific for amino acids 72-91 of atriopeptigen. Sensitivity in the assay is 40 ng atriopeptigen/ml and 0.6 ng atriopeptigen (72-91) per ml. The rabbit antibody does not crossreact with atriopeptin.

  11. Radioimmunoassay for motilin.

    Science.gov (United States)

    Dryburgh, J R; Brown, J C

    1975-05-01

    A specific radioimmunoassay for motilin has been developed with the use of antisera to porcine motilin raised in guinea pigs. Highly purified 125-I-motilin was used as the tracer and the sensitivity range was 10 to 320 pg. No cross-reactivity was demonstrated with gastric inhibitory polypeptide, secretin, glucagon, gastrin, cholecystokinin-pancreozymin, or vasoactive intestinal peptide. In dogs with denervated pouches of the fundus of the stomach and Mann-Bollman fistulae, duodenal alkalinization resulted in an increase in gastric motor activity in the fundic pouch with a corresponding increase in serum motilin.

  12. Indirect radioimmunoassay for interferon

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    Lyons, S.F.; Schoub, B.D. (University of the Witwatersrand, Johannesburg (South Africa). Dept. of Virology); Chiu, M.N.; Crespi, M. (National Institute for Virology, Johannesburg (South Africa))

    1982-10-01

    An indirect radioimmunoassay for testing the antiviral activity of interferon (IFN) is described. Vero cells are seeded in microtitre plates, treated with appropriate dilutions of interferon and challenged with Sindbis virus. Viral yield is measured using specific antibody and radiolabelled protein A. The assay is able to detect IFN levels of 5 international units (I.U.)/ml, has a high degree of reproducibility, and could be easily adapted to various cell and virus combinations. This microsystem is technically simple, allows testing of small volumes of test material, and eliminates subjectivity in reading of endpoints.

  13. Radioimmunoassay in basic and clinical pharmacology

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    Patrono, C.; Peskar, B.A.

    1987-01-01

    The subject of the book is the development, validation and application of radioimmunoassay (RIA) techniques for the measurement of a variety of substances in animal and human body fluids. The book discusses methodological and conceptual issues related to the main classes of mediators of drug action and to drugs themselves, as assayed by this particular analytical technique. A number of introductory chapters provide basic information concerning production and characterization of antibodies, labeling techniques, statistical aspects and validation criteria, insight into problems related to the development and validation of RIA for the newly discovered mediator(s). In the following chapters, the emphasis is placed on the technical details relevant to each class of compounds and on specific aspects of their applications to basic and/or clinical pharmacological studies. New developments in this area, such as monoclonal antibodies and non-radioactive labeling techniques, are also covered.

  14. Specific radioimmunoassay of oxytocin in rat plasma.

    Science.gov (United States)

    Vecsernyés, M; Török, A; Jójárt, I; Laczi, F; Penke, B; Julesz, J

    1994-09-01

    Sensitive, specific and reproducible radioimmunoassay (RIA) was developed for the measurement of oxytocin (OXT) in rat blood plasma after various extraction methods. The assay is based on an antiserum raised against OXT in rabbit. The sensitivity, affinity constant, and cross-reactivity of the antiserum were determined. The 125I-labelled OXT for RIA was produced by chloramine-T method and purified with high pressure liquid chromatography (HPLC). Two extraction procedures were employed: 1. adsorption to an artificial silicate, Lichroprep Si 60 (Merck); 2. immunoextraction of the hormone applying a magnetic bearer covered with purified antibodies against OXT. The specificity of the extraction methods was characterized in comparative HPLC/RIA studies of specimens extracted from blood plasma in different ways. The basal level of the peptide measured after the extraction with thermally activated Lichroprep Si 60 or after the immunoextraction method was found to be 9.6 +/- 2.3 pg/ml (mean +/- S.E.) and 15.3 +/- 0.9 pg/ml (mean +/- S.E.), respectively. Various well known factors (ether exposure, hyperosmotic stress and suckling) appeared to be potent peripheral stimuli of OXT release, and thus indicated the suitability of the RIA method for the measurement of OXT in blood plasma.

  15. Nalmefene: radioimmunoassay for a new opioid antagonist.

    Science.gov (United States)

    Dixon, R; Hsiao, J; Taaffe, W; Hahn, E; Tuttle, R

    1984-11-01

    A specific radioimmunoassay (RIA) has been developed for the quantitation of a new opioid antagonist, nalmefene, in human plasma. The method employs a rabbit antiserum to an albumin conjugate of naltrexone-6-(O-carboxymethyl)oxime and [3H]naltrexone as the radioligand. Assay specificity was achieved by extraction of nalmefene from plasma at pH 9 into ether prior to RIA. The procedure has a limit of sensitivity of 0.2 ng/mL of nalmefene using a 0.5-mL sample of plasma for analysis. The intra- and interassay coefficients of variation did not exceed 5.6 and 11%, respectively. The specificity of the RIA was established by demonstrating excellent agreement (r = 0.99) with a less sensitive and more time consuming HPLC procedure in the analysis of clinical plasma samples. The use of the RIA for the pharmacokinetic evaluation of nalmefene is illustrated with plasma concentration profiles of the drug in humans following intravenous and oral administration.

  16. Bioassay and radioimmunoassay of lactogens in sera from children.

    Science.gov (United States)

    Gout, P W; Tze, W J; Rennie, P S; Bruchovsky, N

    1984-05-16

    Lactogen levels in sera from children have been determined using the Nb 2 lymphoma cell bioassay (BA) and conventional radioimmunoassay (RIA). Assays were done on samples obtained under basal conditions and after pituitary stimulation induced by insulin or arginine administration. There was a close correspondence between BA and RIA results (r = 0.94; n = 43). The average ratio of the BA and RIA estimates of the lactogen levels (BA/RIA) was 0.86 +/- 0.13 (mean +/- SD) and 0.82 +/- 0.17 for basal and stimulated conditions, respectively. The increased secretion of lactogens after pituitary stimulation was not found to be associated with a change in the BA/RIA ratio.

  17. Radioimmunoassay for pyridostigmine

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    Meyer, H.G.; Lukey, B.J.; Gepp, R.T.; Corpuz, R.P.; Lieske, C.N.

    1988-01-01

    Pyridostigmine is a cholinergic drug used for the treatment of myasthenia gravis and for antagonizing the effects of non-depolarizing muscle relaxants. In addition, military organizations in several countries have an active interest in pyridostigmine as a pretreatment compound for nerve agent poisoning. Anti-pyridostigmine antibodies were produced in rabbits using a pyridostigmine analog conjugated to keyhole limpet hemocyanin. These antibodies were used for development of a radioimmunoassay that has a linear standard curve (r2=0.986) ranging from 0.5 to 10.0 ng/ml of pyridostigmine bromide in a 0.1-ml plasma sample. This assay measures pyridostigmine in plasma with better sensitivity and much greater through-put than do current state-of-the-art high-performance liquid chromatography techniques. In addition, only small volumes (100ml) of the plasma samples are required. Plasma levels of pyridostigmine were determined in the rat after intramuscular administration (0.056mg/kg) of pyridostigmine bromide. Estimates of the various pharmacokinetic parameters were calculated using the computer program NONLIN84. The results were as follows: apparent volume of distribution = 1.97 1/kg, absorption rate constant = 0.277 min-1, elimination rate constant = 0.0273 min-1, area under the curve = 1010 ng x min/ml, absorption rate half-life = 2.41 min, elimination rate half-life = 24.8 min, maximal plasma concentration (Cmax) = 21.3 ng/ml and time to Cmax = 9.02 min.

  18. Diagnostic value of prostatic acid phosphatase as determined by radioimmunoassay

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    Wirth, M.P.; Osterhage, H.R.; Ackkermann, R.

    1981-07-01

    Serum concentrations of prostatic acid phosphatase (PAP) were determined with 4 different radioimmunoassays and with the standard enzymatic method (p-nitrophenylphosphate) in 35 patients with prostatic carcinoma. Staging of localized tumors was based on histopathological evaluation after radial prostatectomy and pelvic lymphnode dissection (pTsub(1-3), pN/sub 0/). In tumor lesions Tsub(1-2) N/sub 0/ M/sub 0/ elevated PAP-serum concentrations were found by RIA-determination in only one patient. Increased PAP serum levels were observed in 43-78% of carcinomas stage T/sub 3/ N/sub 0/ M/sub 0/ and in 54-83% in stage Tsub(2-4) Nsub(x) M/sub 1/ tumors, depending on the test kit used for the PAP determination. Concentrations for PAP obtained with the 4 different RIA-kits used, varied significantly and thus are not comparable. No false positive results were observed in sera of 9 patients with benign prostatic hyperplasia. Elevated PAP serum levels were found in a significantly higher frequency when determined by radioimmunoassay than by the enzymatic method. The results clearly indicate, that PAP is of no value for early recognition of carcinoma of the prostate even when measured by radioimmunoassay. However, the RIA-method seems to be of clinical importance in estimating the course of advanced local and metastasizing carcinoma of the prostate.

  19. Comparison of VIDAS and Radioimmunoassay Methods for Measurement of Cortisol Concentration in Bovine Serum

    OpenAIRE

    Daniela Proverbio; Roberta Perego; Eva Spada; Giada Bagnagatti de Giorgi; Angelo Belloli; Davide Pravettoni

    2013-01-01

    Radioimmunoassay (RIA) is the “gold standard” method for evaluation of serum cortisol concentration. The VIDAS cortisol test is an enzyme-linked fluorescent assay designed for the MiniVidas system. The aim of this study was to compare the VIDAS method with RIA for measurement of bovine serum cortisol concentration. Cortisol concentrations were evaluated in 40 cows using both VIDAS and RIA methods, the latter as the reference method. A paired Student's t-test, Pearson's correlation analysis, B...

  20. Microcomputer in a radioimmunoassay laboratory

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    Baud, M.; Cardot, J.C.; Verdenet, J.; Daspet, J.P.; Bidet, R.

    1986-01-01

    Data processing is currently being used in Radioimmunoassay laboratories. The software which is described here fits the present recommendations for the statistical evaluation of results: confidence limit, precision profile, validation and intra and inter-series quality control. On the other hand it facilitates the administrative work linked to the reception of samples and to the dispatching of results.

  1. Immunoradiometric assay for prolactin in serum and tissue; Comparison with radioimmunoassay

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    Ohnami, Shumpei; Nakata, Hajime; Eto, Sumiya (University of Occupational and Environmental Health Hospital, Kitakyushu, Fukuoka (Japan))

    1990-09-01

    Prolactin (PRL) concentrations in sera and tumors of patients with various pituitary tumors were measured by both immunoradiometric assay (IRMA) and radioimmunoassay (RIA). PRL concentrations in sera and tumor tissues measured by IRMA were well correlated with those measured by RIA. PRL concentrations in sera reflected those of tumors removed. This IRMA is a simple and useful method for PRL determination in serum and tissue. (author).

  2. Radioimmunoassay of pepsinogens I and Ⅱin human serum

    Institute of Scientific and Technical Information of China (English)

    XiaoZhi-Jian; JiangMeng-Jun; 等

    1998-01-01

    Radioimmunoassay(RIA) for pepsinogens I and II(PGI and II)in serum is developed by using the purified PG I and II from human gastric mucosa.The assay range was 8-2560644g/L for PG I and 2-640644g/L for PGⅡ,the sensitivity was 1.3 and 0.6μg/L,respectively.The reproducibilities of PGI-RIA(106.9%) and PGⅡ-RIA(106.7%)are quite well.The within-and detween-assay coefficient of variation(CV) of each RIA was 5.1% and 6.3% for PGI,7.2% and 8.9% for PGⅡ,The serum PG Ⅰlevel in healthy volunteer was 60.41±14.98μg/L(mean±SD).significantly higher than the serum PGⅡ level which was 20.70±9.64μg/L.Therefore,the PGI-RIA and PGⅡ-RIA are useful tools in studying the relation between serum pepsinogen levels and various peptic disorders.

  3. Preparation and purification of 7-Iodoclonazepam for use in radioimmunoassay

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    Goddard, C.P.; Law, B.; Mason, P.A.; Stead, A.H.

    1986-04-01

    A method is described for the preparation and purification of 7-(/sup 125/I)-Iodoclonazepam (5-(o-Chlorophenyl)-2,3-Dihydro-7-(/sup 125/I)-Iodo-1H-1,4-Benzodiazepin-2-one). The structure was confirmed by mass spectrometry using 7-(/sup 127/I)iodoclonazepam prepared by the same method. 7-(/sup 125/I)-Iodoclonazepam binds well to a benzodiazepine antiserum. Although readily displaced by all the benzodiazepines commercially available in the UK, it is not displaced by structurally related nonbenzodiazepines except at very high concentrations. 7-(/sup 125/I) Iodoclonazepam should therefore be useful for the development of a screening radioimmunoassay (RIA) for benzodiazepines.

  4. QC in RIA

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    Little, J. (North East Thames Regional Immunoassay Service (NETRIA), Saint Bartholomew' s Hospital, London (UK))

    1989-09-01

    A Regional Health Authority expert from the service which performs radioimmunoassays (RIA) and immunoradiometric assays (IRMA) discusses the importance of quality control in these techniques. Originally developed as an aid to endocrinology, applications are now much more widely based and include virology, microbiology, drugs testing, immunology, parasitology, veterinary science and the food industry. The origins of errors in the practice of RIA are examined, with and between batches and the limitations placed on accuracy are explained. Measurement variability between laboratories is also mentioned. (U.K.).

  5. A radioimmunoassay for lignin in plant cell walls

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    Dawley, R.M.

    1989-01-01

    Lignin detection and determination in herbaceous tissue requires selective, specific assays which are not currently available. A radioimmunoassay (RIA) was developed to study lignin metabolism in these tissues. A {beta}-aryl ether lignin model compound was synthesized, linked to keyhole limpet hemocyanin using a water-soluble carbodiimide, and injected into rabbits. The highest titer of the antiserum obtained was 34 {eta}g/mL of model derivatized BSA. An in vitro system was developed to characterize the RIA. The model compound was linked to amino activated polyacrylamide beads to mimic lignin in the cell walls. {sup 125}I Radiolabelled protein A was used to detect IgG antibody binding. The RIA was shown in the in vitro system to exhibit saturable binding. The amount of antibody bound decreased when the serum was diluted. Immunoelectrophoresis and competitive binding experiments confirmed that both aromatic rings of the lignin model compound had been antigenic. Chlorogenic acid, a phenolic known to be present in plant cells, did not compete for antibody binding. The RIA was used to measure lignin in milled plant samples and barley seedlings. Antiserum binding to wheat cell walls and stressed barley segments was higher than preimmune serum binding. Antibody binding to stressed barley tissue decreased following NaClO{sub 2} delignification. The RIA was found to be less sensitive than expected, so several avenues for improving the method are discussed.

  6. Methadone radioimmunoassay: two simple methods

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    Robinson, K.; Smith, R.N. (Metropolitan Police Forensic Science Laboratory, London (UK))

    1983-09-01

    Two simple and economical radioimmunoassays for methadone in blood or urine are described. Haemolysis, decomposition, common anticoagulants and sodium fluoride do not affect the results. One assay used commercially-available (1-/sup 3/H)(-)-methadone hydrobromide as the label, while the other uses a radioiodinated conjugate of 4-dimethylamino-2,2-diphenylpentanoic acid and L-tyrosine methyl ester. A commercially-available antiserum is used in both assays. Normethadone and ..cap alpha..-methadol cross-react to a small extent with the antiserum while methadone metabolites, dextropropoxyphene, dipipanone and phenadoxone have negligible cross-reactivities. The 'cut-offs' of the two assays as described are 30 and 33 ng ml/sup -1/ for blood, and 24 and 21 ng ml/sup -1/ for urine. The assay using the radioiodinated conjugate can be made more sensitive if required by increasing the specific activity of the label.

  7. Bleomycin clinical pharmacology by radioimmunoassay

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    Hall, S.W.; Strong, J.E.; Broughton, A.; Frazier, M.L.; Benjamin, R.S.

    1982-01-01

    Bleomycin pharmacokinetics were studied by radioimmunoassay in 11 patients who received 7-30 U intravenously (IV) and eight patients who received 4-30 U subcutaneously (SC). For patients who received IV bleomycin plasma disappearance was biphasic, with a mean initial half-life of 0.26 h and a terminal half-life of 2.3 h. Mean plasma drug clearance was 67.8 ml/min/m/sup 2/ and the volume of distribution was 13.2 l/m/sup 2/. Urinary excretion accounted for 63.9% of the drug in 24 h. After SC administration peak plasma levels occurred in 1.1 h, with a mean elimination half-life of 4.3 h. Mean plasma drug clearance was 60.5 ml/min/m/sup 2/ and the volume of distribution was 19.2 l/m/sup 2/. Bleomycin plasma clearance correlated well with serum creatinine (r/sup 2/=0.72). Bleomycin has a rapid plasma elimination and urinary excretion. Bleomycin bioavailability after SC administration appears comparable to that seen after IV administration as determined by the areas under the plasma disappearance curves. Prolonged plasma levels are seen after SC injection, suggesting this route of administration can produce plasma concentrations comparable to those attained with continuous IV infusions.

  8. A critical evaluation of a specific radioimmunoassay for prostatic acid phosphatase

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    Goldenberg, S.L.; Silver, H.K.; Sullivan, L.D.; Morse, M.J.; Archibald, E.L.

    1982-11-01

    A radioimmunoassay (RIA) method for acid phosphatase detection was compared to a standard enzyme assay using sera from 210 normal volunteers and 285 patients with prostatic disease. Statistical and clinical comparisons were made between defined subgroups. All 55 normal females had RIA detectable serum acid phosphatase, implying that this assay cannot be entirely specific for enzyme of prostatic origin. Urinary catheterization did not affect acid phosphatase levels. In all stages of carcinoma there were more acid phosphatase elevations by the RIA method than enzyme method, but neither assay could differentiate intercapsular cancer from benign prostatic hyperplasia. A small number of patients with biopsy proven negative nodules had marginally elevated values, suggesting an obligation for closer follow-up. The RIA method may be superior for monitoring patients with more advanced malignancy. Additional practical advantages of the RIA include relative simplicity and elimination of the special serum handling required for the enzyme assay.

  9. Sensitivity and specificity of eicosanoid radioimmunoassays: a new strategy

    Energy Technology Data Exchange (ETDEWEB)

    Dray, F. (Institut Pasteur, 75 - Paris (France))

    1983-07-01

    In radioimmunoassays (RIAs) the limit of detection of the unknown (ligand, competitor) depends first on the affinity of antibody populations engaged in the immunological reaction at a given dilution of the antiserum, second on the specific radioactivity of the tracer or inhibitor, and third on the degree of structural homology of both components (unknown and tracer) of the competition. The commercially available tritiated eicosanoids have up to eight atoms of tritium incorporated into their molecules, allowing RIAs with convenient sensitivities to be developed. Often, however, a higher sensitivity is needed in several studies. First attempts (Dray et al., 1972) using PGFsub(2..cap alpha..) covalently bound to bacteriophage T/sub 4/ as a tracer yielded a highly sensitive viroimmunoassay (limit of detection, less than 1 pg per tube), but its wider application requires further studies, particularly to reduce the non-specific binding. On the other hand, the authors succeeded in developing very sensitive and reliable RIAs using iodinated derivatives, which could be applied to any eicosanoid.

  10. Radioimmunoassay of Urinary Free Cortisol.

    Science.gov (United States)

    1976-09-28

    RIA determination of T issue cortisol levels as a function of psy- cortisol in human serum or plasma is sim-chological or physical stress have been...lo remove cross reacting materia ls and of— lution ( I : 9. v, v . 1 ransfe r 9 ml to a oi- lers t he con’, eni c nce ot us ing a commercial umet rie...dclerminitiions on plasma ~ ht,n the procedure rec-Radiocortisol working solution is pre- ommcndcd (is RtA of Catifornia is used i~ . 6 134 VOL. 14 . NO. 2, H.L.S

  11. Immunodiagnosis of systemic candidiasis: mannan antigenemia detected by radioimmunoassay in experimental and human infections.

    Science.gov (United States)

    Weiner, M H; Coats-Stephen, M

    1979-12-01

    A radioimmunoassay (RIA) that detects candida mannan was developed so that immunodiagnosis of systemic candidiasis could be improved. The RIA was evaluated in an animal model of disseminated disease and in a panel of patient sera. Mannan antigenemia was detected with the RIA in 52% of 29 rabbits with systemic candidasis, but not in 60 normal rabbits or 31 rabbits with systemic aspergillosis. In an evaluation of human sera, mannan antigenemia was detected in five of 11 patients with systemic candidiasis, one of three patients with invasive gastrointestinal candidiasis, and one patient with a sustained candidemia associated with an infected intravenous catheter. Mannan was not detected in sera from 11 patients with superficial candida infections, seven patients colonized with Candida, three patients with chronic mucocutaneous candidiasis, eight patients with other systemic mycoses, or 22 normal donors. This study demonstrates the utility of this RIA for early, specific immunodiagnosis of invasive candidiasis.

  12. Quantification of rat kisspeptin using a novel radioimmunoassay.

    Directory of Open Access Journals (Sweden)

    James S Kinsey-Jones

    Full Text Available Kisspeptin is a hypothalamic peptide hormone that plays a pivotal role in pubertal onset and reproductive function. Previous studies have examined hypothalamic kisspeptin mRNA expression, either through in situ hybridisation or real-time RT-PCR, as a means quantifying kisspeptin gene expression. However, mRNA expression levels are not always reflected in levels of the translated protein. Kisspeptin-immunoreactivity (IR has been extensively examined using immunohistochemistry, enabling detection and localisation of kisspeptin perikaya in the arcuate nucleus (ARC and anteroventral periventricular nucleus (AVPV. However, quantification of kisspeptin-IR remains challenging. We developed a specific rodent radioimmunoassay assay (RIA capable of detecting and quantifying kisspeptin-IR in rodent tissues. The RIA uses kisspeptin-10 as a standard and radioactive tracer, combined with a commercially available antibody raised to the kisspeptin-10 fragment. Adult female wistar rat brain samples were sectioned at 300 µm and the ARC and AVPV punch micro-dissected. Brain punches were homogenised in extraction buffer and assayed with rodent kisspeptin-RIA. In accord with the pattern of kisspeptin mRNA expression, kisspeptin-IR was detected in both the ARC (47.1±6.2 fmol/punch, mean±SEM n = 15 and AVPV (7.6±1.3 fmol/punch, mean±SEM n = 15. Kisspeptin-IR was also detectable in rat placenta (1.26±0.15 fmol/mg. Reverse phase high pressure liquid chromatography analysis showed that hypothalamic kisspeptin-IR had the same elution profile as a synthetic rodent kisspeptin standard. A specific rodent kisspeptin-RIA will allow accurate quantification of kisspeptin peptide levels within specific tissues in rodent experimental models.

  13. Enzymatic tracer damage during the gonadotropin releasing hormone radioimmunoassay: analytical and immunological assessment

    Energy Technology Data Exchange (ETDEWEB)

    O' Conner, J.L.; Lapp, C.A.; Clary, A.R.

    1985-09-23

    Hypothalamic supernatants from 60 day female rats were fractionated from Sephadex G-200 columns. The radioimmunoassay (RIA) for gonadotropin releasing hormone (GnRH) detected an apparently cross-reacting high molecular weight substance. The substance caused apparent displacement of iodinated GnRH binding in dose response fashion; however, no biological activity was observed in pituitary cell cultures. In order to determine whether the depressed binding might be caused by enzymatic degradation of iodinated GnRH during the RIA incubation, iodinated GnRH was preincubated under RIA conditions with either buffer or increasing concentrations of the GnRH cross-reacting material. Aliquots were subjected to polyacrylamide gel electrophoresis (PAGE) and the gels slices counted. Identical aliquots were subsequently used as iodinated hormone in the RIA of known quantities of synthetic GnRH. Tracer damage during the RIA-like preincubation period was reflected in the subsequent PAGE studies as decreased counts per minute in the intact GnRH peak and in the RIA studies as over-estimated quantification of the GnRH standards. This report describes such damage during the GnRH RIA and the data misinterpretations which result. 30 references, 6 figures, 1 table.

  14. Solid phase radioimmunoassay using labelled antibodies: a conceptual framework for designing assays

    Energy Technology Data Exchange (ETDEWEB)

    Kalmakoff, J.; Parkinson, A.J.; Crawford, A.M.; Williams, B.R.

    1977-01-01

    A simple theoretical model for the antigen-antibody reaction is presented and used to evaluate the optimum conditions for designing solid phase radioimmunoassay (RIA) using labelled antibodies. Both theoretical and experimental data are presented, using a wide variety of antigens and their corresponding antibodies. The types of RIA described include the direct, the indirect, and sandwich assays for detecting either antigen or antibody. The experimental results confirm in a semiquantitative manner that the greatest sensitivity of the RIA is achieved when the smallest amount of labelled antibody is used, that whenever possible the antigen/antibody ratio should be greater than unity (greater than 1), and that the formation of the antigen-antibody complex is dependent on the mass action effect.

  15. Anamnesis of the radioimmunoassay: A historical reminiscence

    Energy Technology Data Exchange (ETDEWEB)

    Kallee, E.

    1984-01-27

    Anamnesis of the Radioimmunoassay - A Historical Reminiscence: Several requirements have to be met before the first radioimmunoassay of a proteohormone (i.e., insulin) could be developed. One of these requirements was that iodination ought not substantially impair the immunologic properties of the insulin molecule. Since the reversibility of antigen adsorption to antibodies is the most important basis of all immunoassays, the adsorptive behavior of proteins first had to be studied at extremely low protein concentrations. Only after these requirements had been investigated was direct electrophoretic detection of a nonprecipitating anti-hormone antibody possible by desorption of insulin on filter paper. Later, the inaccuracy of the species specificity of insulin-binding antibodies proved to be particularly useful for the determination of insulin in human sera.

  16. Radioimmunoassays - what is their diagnostic value

    Energy Technology Data Exchange (ETDEWEB)

    Panitz, N.

    1983-09-16

    A selection is made of hormones and enzymes which are measurable by radioimmunoassay and their organ-specific function is described. Endocrine function tests together with the stimulating or suppressing substances, reaction in the organism and the examined regulation are listed. Non-radioactive immunological determination methods and the use of monoclonal antibodies in vitro and in vivo with ''tumor imaging'' are indicated.

  17. Radioimmunoassay of measles virus hemagglutinin protein G

    Energy Technology Data Exchange (ETDEWEB)

    Lund, G.A.; Salmi, A.A. (Turku Univ. (Finland))

    1982-08-01

    Guinea pig and rabbit antisera from animals immunized with purified measles virus hemagglutinin (G) protein were used to establish a solid-phase four-layer radioimmunoassay for quantitative measurement of the G protein. The sensitivity of the assay was 2 ng of purified G protein, and 200 ..mu..g of protein from uninfected Vero cells neither decreased the sensitivity nor reacted non-specifically in the assay. Radioimmunoassay standard dose-response curves were established and unknown values interpolated from these using the logit program of a desktop computer. Using this procedure, a measles virus growth curve in infected Vero cells was determined by measurement of G protein production. Under these same conditions, hemagglutination was not sensitive enough to detect early hemagglutinin production. Viral antigens in canine distemper virus, Newcastle disease virus, parainfluenza viruses 1-4, simian virus 5, and respiratory syncytial virus-infected cell lysates did not cross-react in the radioimmunoassay. A small degree of cross-reactivity was detected with mumps viral antigens, both with Vero cell-derived (wild-type strain) and egg-derived (Enders strain) purified virus preparations and with a cell lysate antigen prepared from wild-type mumps virus-infected Vero cells.

  18. Creatine kinase B subunit as measured with a radioimmunoassay kit in detection of acute myocardial infarction.

    Science.gov (United States)

    Witherspoon, L R; Shuler, S E; Genre, C F; Gilbert, S S; Moore, R J; Meihaus, V; Hurry, E K

    1983-02-01

    Results with a commercial radioimmunoassay (RIA) reagent kit for quantification of the creatine kinase B subunit (CK-B) (Nuclear-Medical Laboratories, Irving, TX 75061) were compared with results obtained by electrophoresis for patients consecutively admitted to our coronary care unit for suspected acute myocardial infarction. Analytical sensitivity, precision, and specificity of the RIA were satisfactory. Its clinical efficacy was assessed in 97 patients suspected of having had an acute myocardial infarction. Of 30 patients who had had an acute myocardial infarction, increased CK-B was detected by RIA in 30 and by electrophoresis in 27. The temporal relationship between CK-B by RIA and CK-MB by electrophoresis was similar. Of 66 admissions where infarction was not established, CK-B was negligibly increased in samples from four patients by RIA, and from one by electrophoresis. Although not abnormally increased (greater than 5 U/L), CK-MB was detected by electrophoresis in samples from another five of these 66 patients. We conclude that estimation of CK-B by this RIA is an excellent alternative to estimation of CK-MB by electrophoresis in patients suspected of having had an acute myocardial infarction.

  19. Comparison of indirect immunofluorescence and radioimmunoassay for detecting antibody to varicella-zoster virus

    Energy Technology Data Exchange (ETDEWEB)

    Campbell-Benzie, A.; Heath, R.B.; Ridehalgh, M.K.S.; Cradock-Watson, J.E. (Saint Bartholomew' s Hospital, London (UK))

    1983-03-01

    Immunofluorescence (IF) and radioimmunoassay (RIA) were found to be more sensitive methods than complement fixation (CF) for detecting antibody to varicella-zoster (V-Z) virus. RIA yielded titres about 30 times greater than those obtained by IF, but for screening purposes RIA was only about six times more sensitive since the minimum serum dilutions that could be tested were 1/100 and 1/16 respectively. When 539 sera from subjects of different ages were screened for V-Z antibody, IF and RIA gave concordant results with 527 specimens (98%). When 19 patients were tested who had not previously had varicella but were experiencing primary infection with herpes simplex (HS) virus, crossreacting antibodies to V-Z antigens were detected in six patients by IF but in only two of these by RIA. IF and RIA are preferable to CF as tests for immune status because of their greater sensitivity, but weak positive reactions caused by presumptive low titres of homologous antibody or by higher titres of heterologous antibody can occur in one or both tests. Such reactions could cause difficulty in assessing the need for vaccine or for specific immune globulin, and in interpreting the response to vaccination.

  20. Radioimmunoassay of sodium cromoglycate in human plasma

    Energy Technology Data Exchange (ETDEWEB)

    Brown, K.; Gardner, J.J.; Lockley, W.J.S.; Preston, J.R.; Wilkinson, D.J. (Fisons plc, Loughborough (UK). Pharmaceutical Div.)

    1983-01-01

    A sensitive radioimmunoassay method for sodium cromoglycate in human plasma is described. The lowest quantifiable concentration of sodium cromoglycate is 0.93 nmol/l when 0.1 ml plasma samples are analysed. The range of the method is limited; both 0.01 and 0.1 ml volumes of plasma must be analysed to encompass the concentration range 0.93-139 nmol/l which may be encountered in plasma samples from patients and human volunteers. The method is specific for sodium cromoglycate as indicated by a low cross-reactivity of the anti-cromoglycate antiserum with a number of drugs.

  1. Radioimmunoassay for motilin. [/sup 125/I

    Energy Technology Data Exchange (ETDEWEB)

    Dryburgh, J.R.; Brown, J.C.

    1975-01-01

    A specific radioimmunoassay for motilin has been developed with the use of antisera to porcine motilin raised in guinea pigs. Highly purified /sup 125/I-motilin was used as the tracer and the sensitivity range was 10 to 320 pg. No cross-reactivity was demonstrated with gastric inhibitory polypeptide, secretin, glucagon, gastrin, cholecystokininpancreozymin, or vasoactive intestinal peptide. In dogs with denervated pouches of the fundus of the stomach and Mann-Bollman fistulae, duodenal alkalinization resulted in an increase in gastric motor activity in the fundic pouch with a corresponding increase in serum motilin.

  2. Novel /sup 125/I radioimmunoassay for the analysis of. delta. /sup 9/-tetrahydrocannabinol and its metabolites in human body fluids

    Energy Technology Data Exchange (ETDEWEB)

    Law, B.; Mason, P.A.; Moffat, A.C.; King, L.J.

    A cannabinoid radioimmunoassay (RIA) that detects some of the major ..delta../sup 9/-THC metabolites is developed and evaluated for use in forensic science. It incorporates a novel /sup 125/I radiotracer, is sensitive, reliable, relatively quick, and simple to use. The RIA uses a commercially available antiserum and detects a number of cannabinoid metabolites, including ..delta../sup 9/-THC-11-oic acid and its glucuronide conjugate in biological fluids. The method was successfully applied to the analysis of blood and urine samples submitted for forensic analysis.

  3. Heterologous radioimmunoassay of 19-nortestosterone and trenbolone

    Energy Technology Data Exchange (ETDEWEB)

    Vogt, K.

    Using /sup 3/H-methyltrienolone (86 Ci/mmol) in a heterologous radioimmunoassay instead of /sup 3/H-19-nortestosterone (19 Ci/mmol) in the generally used homologous system the sensitivity of the test could be improved 7-fold (50% relative binding by 15 picograms of 19-nortestosterone). Due to the relatively high cross reactivity for trenbolone of about 35% (with a different antiserum 50% have been found for trenbolone-17..cap alpha..) it is possible to have a simultaneous control of that illegal anabolic. In case of suspicion the substances responsible for the positive radioimmunoassay can be verified with a very specific assay for trenbolone. A preliminary examination of the findings via determination of 'internal blanc values' has proved to be a good measure again. With the test using the previously described extraction and clean up procedures prosecution limits of 0.1 ng/g muscle, 1 ng/ml urine and 10 ng/ml bile might be reasonable. The blanc values depend significantly on the colour of urine samples and on the enzyme used for hydrolysis (glucuronidase from Escherichia coli gave no additional blanc in contrast to succ. helix pomatioa).

  4. Radioimmunoassay in the diagnosis of arterial hypertension

    Energy Technology Data Exchange (ETDEWEB)

    Slavnov, V.N.; Olejnik, V.A.; Yakovlev, A.A.; Yugrinov, O.G.; Markov, V.V. (Kievskij Nauchno-Issledovatel' skij Inst. Ehndokrinologii i Obmena Veshchestv (Ukrainian SSR))

    1984-11-01

    The paper is concerned with the results of a study of the aldosterone concentration and renin activity, the general level of catecholamines and their fractions in the peripheral blood and blood taken at selective venography from the vena cava inferior, renal and adrenal veins of 108 patients with hypertension, aldosteroma and idiopathic hyperaldosteronism, adrenal and extraadrenal pheochromocytoma, renovascular and renoparenchymatous arterial hypertension. The aldosterone concentration and renin activity were determined with radioimmunoassay, and the general content of catecholamines and their fractions with a radioenzymatic method using standard kits. It has been shown that the radioimmunoassay to determine the aldosterone concentration and renin activity makes possible differential diagnosis of hypertension, aldosteroma, idiopathic and secondary hyperaldosteronism. A considerable increase in the blood plasma renin activity on the affected side was revealed in the patients with renovascular hypertension, and in renoparenchymatous hypertension it was equal in both renal veins. The study of the total content of catecholamines and their fractions in the blood from different parts of the venous system can be used for topical diagnosis of adrenal and extraadrenal pheochromocytoma.

  5. Optimization of the radioimmunoassays for measuring fentanyl and alfentanil in human serum

    Energy Technology Data Exchange (ETDEWEB)

    Schuettler, J.; White, P.F.

    1984-09-01

    Measurement of serum fentanyl and alfentanil concentrations by radioimmunoassay (RIA) may result in significant errors and high variability when the technique described in the available fentanyl and alfentanil RIA kits is used. The authors found a 29-94% overestimation of measured fentanyl and alfentanil serum levels when 3H-fentanyl or 3H-alfentanil was added lastly to the mixture of antiserum and sample. This finding is related to a reduction in binding sites for the labeled compounds after preincubation of sample and antiserum. If this sequence is used, it becomes necessary to extend the incubation period up to 6 h for fentanyl and up to 10 h for alfentanil in order to achieve equilibration between unlabeled and labeled drug with respect to antiserum binding. However, when antiserum is added lastly to the mixture of sample and labeled drug, measurement accuracy and precision for fentanyl and alfentanil serum concentrations are enhanced markedly. In addition, it is important to perform the calibration curves and sample measurements using the same medium (i.e., serum alone or a serum/buffer dilution). In summary, to optimize the RIA for fentanyl and alfentanil, the authors recommend the following: 1) adding the antiserum lastly to the mixture of sample and labeled drug; 2) performing calibration curves using patient's blank serum when possible; 3) carefully examining and standardizing each step of the RIA procedure to reduce variability, and, finally; 4) comparing results with those of other established RIA laboratories.

  6. NON-RADIOMETRIC IMMUNOASSAYS [FLUOROIMMUNOASSAY (FIA AND FLUOROMETRIC ENZYME IMMUNOASSAY (FEIA] WITH RADIOIMMUNOASSAY (RIA FOR EVALUATION OF ADRENAL FUNCTION IN NORMAL AND HYPERCORTISOLEMIC DOGS MÉTODOS DE IMUNOENSAIO NÃO RADIOMÉTRICOS [FLUOROIMUNOENSAIO (FIE E ENZIMAIMUNOENSAIO (EIE] E O RADIOIMUNOENSAIO (RIE NA AVALIAÇÃO DA FUNÇÃO ADRENAL DE CÃES NORMAIS E CÃES COM HIPERADRENOCORTICISMO

    Directory of Open Access Journals (Sweden)

    Márcia Marques Jericó

    2002-04-01

    Full Text Available Non-radiometric immunoassays offer many advantages over radiometric assays, such as higher stability of kit compounds and absence of potential hazardous effects for users and environment. The comparison of cortisol measurements by fluoroimmunoassay (FIA and fluorometric enzyme immunoassay (FEIA with radioimmunoassay (RIA in adrenal function evaluation of normal (n=50 and hypercortisolemic dogs (n=12 was proposed. Serum concentrations of cortisol were measured in basal conditions and 8 hours after dexamethasone (DEX suppression (0.01mg/kg/IV. All our reference values were based on the 5th and 95th percentile. The values for basal cortisol of healthy dogs were 0.20 to 2.35mug/d for FIA, 0.30 to 5.39mug/d for FEIA, and 0.65 to 4.64mug/d for RIA. After DEX suppression the values were , and for FIA, FEIA and RIA, respectively. In hypercortisolemic dogs, the values of cortisol (mean ± SD in basal and post-DEX conditions were 2.71 + 0.41mug/d and 1.73 + 1.15mug/d for FIA, 7.05 + 2.85mug/d and 4.93 + 2.26mug/d for FEIA, and 4.80 + 1.43mug/d and 3.52 + 1.08mug/d for RIA. Statistically significant differences (pOs métodos de dosagem hormonal por técnicas não-radioativas apresentam inúmeras vantagens sobre os que utilizam radioisótopos como marcadores de hormônios ou anticorpos. Dentre tais vantagens, incluem-se maior meia-vida útil dos reagentes por maior estabilidade dos compenentes, inexistência de riscos de contaminação radioativa, tanto pessoal quanto ambiental. Para avaliar a aplicação destes novos métodos na prática da endocrinologia clínica de pequenos animais, comparamos o método de radioimunoensaio (RIE aos métodos alternativos fluoroimunoensaio (FIE e enzimaimunoensaio (EIE na avaliação da função adrenal canina. Para tanto, padronizaram-se os níveis de cortisol em cães normais (n=50 e em cães com hiperadrenocorticismo (n=12, sob condições basais e oito horas após supressão com dexametasona (DEX (0,01mg/kg IV. Os

  7. Radioimmunoassay of sodium cromoglycate in human plasma

    Energy Technology Data Exchange (ETDEWEB)

    Brown, K.; Gardner, J.J.; Lockley, W.J.S.; Preston, J.R.; Wilkinson, D.J. (Fisons Ltd., Loughborough (UK). Pharmaceutical Div.)

    1983-01-01

    A sensitive radioimmunoassay method for sodium cromoglycate in human plasma is described. The lowest quantifiable concentration of sodium cromoglycate is 0.93 nmol/1 when 0.1 ml plasma samples are analysed. Direct analysis of sodium cromoglycate concentrations in plasma samples collected up to several hours after the administration of single therapeutic doses of the compound is possible. The range of the method is limited; both 0.01 and 0.1 ml volumes of plasma must be analysed to encompass the concentration range 0.93-139 nmol/1 which may be encountered in plasma samples from patients and human volunteers. The method is specific for sodium cromoglycate as indicated by a low cross-reactivity of the anti-cromoglycate antiserum with a number of drugs.

  8. Clinical studies of IGFBP-2 by radioimmunoassay

    DEFF Research Database (Denmark)

    Blum, W F; Horn, N; Kratzsch, J

    1993-01-01

    A specific radioimmunoassay for human IGFBP-2 was developed using a polyclonal antiserum directed against a partial sequence (hIGFBP-2(176-190)). The tracer was prepared by radioiodination of a [Tyr]o-hIGFBP-2(176-190) derivative. The assay was used to study IGFBP-2 levels in numerous clinical...... and experimental situations. There was little circadian fluctuations of serum level which showed a marked age-dependence with high levels at birth and senescence and low levels during puberty. Decreased IGFBP-2 levels were present in untreated insulin-dependent diabetes mellitus (IDDM), in acromegaly and during...... dexamethasone suppression test. GH deficiency, fasting, IGF-I administration to patients with GH receptor deficiency, hepatic failure and insulinomas caused a moderate increase of serum IGFBP-2. Markedly elevated levels were found in chronic renal failure, non-islet cell tumour induced hypoglycemia...

  9. Radioimmunoassay of secretin in human serum

    Energy Technology Data Exchange (ETDEWEB)

    Bonora, G.; Vezzadini, P.; Toni, R.; Labo, G. (Bologna Univ. (Italy))

    1984-06-27

    A sensitive radioimmunoassay for secretin has been developed. Antisera were raised against synthetic porcine secretin coupled to bovine serum albumin. N-..cap alpha..-desaminotyrosyl-..beta..-alanyl secretin was radioiodinated by a slight modification of the chloramine-T method. Pure synthetic porcine secretin was used as a standard. Free and bound hormone were separated by dextran-coated charcoal. No cross-reactivity was found with structurally and physiologically related peptides. The sensitivity of the assay was high enough to measure fasting secretin levels in human serum. Patients with acute or chronic pancreatitis had mean serum secretin concentration not significantly different from healthy subjects. In patients with pancreatic carcinoma the mean serum secretin concentration was significantly lower than in healthy subjects, although a wide overlap of the two groups was evident.

  10. Radioimmunoassay for 21-deoxycortisol: clinical applications

    Energy Technology Data Exchange (ETDEWEB)

    Gueux, B.; Fiet, J.; Villette, J.M.; Brerault, J.L.; Vexiau, P.; Julien, R. (Laboratoire de Biochimie Hormonale, Hopital Saint-Louis, Paris); Pham-Huu-Trung, M.T.; Gourmelen, M. (INSERM U 142 and Laboratoire d' Explorations Fonctionnelles, Hopital Trousseau, Paris); Galons, H. (Laboratoire de Chimie Organique, Faculte de Pharmacie, Paris)

    1985-01-01

    A radioimmunoassay for 21-deoxycortisol is described. The immunogen, 21-deoxycortisol-3-(0-carboxymethyl) oxime-bovine serum albumin, was prepared, the antisera raised against it were studied and the reliability of the assay was checked. The antiserum selected cross-reacted with 11-deoxycortisol (0.08%), corticosterone (0.25%), cortisol (0.6%) and 17-hydroxyprogesterone (1.6%). 21-deoxycortisol was separated by celite partition chromatography and eluted in the 70/30 (v/v) isooctane/ethyl acetate fraction together with 11-deoxycortisol and corticosterone. The radioimmunoassay was used to measure 21-deoxycortisol in the plasma of normal subjects and patients with androgen excess. In normal subjects, men (0.19 ng/ml +/- 0.08) and women (0.18 ng/ml +/- 0.09) had similar basal levels (mean +/- SD). One hour after ACTH stimulation, these levels were increased by a factor of 3.5. In 7 patients treated for classical congenital adrenal hyperplasia associated with 21-hydroxylase deficiency, basal values varied between 9.1 and 39.9 ng/ml (measured at 8 a.m.). In 7 untreated women with late-onset congenital adrenal hyperplasia (with 21-hydroxylase deficiency), ACTH-stimulated levels were increased to between 9 and 25.5 ng/ml. In 14 heterozygous carriers of 21-hydroxylase deficiency, diagnosed by HLA genotyping, all ACTH-stimulated levels were well above the highest corresponding levels in normal subjects, whereas 17-hydroxyprogesterone levels remained within the normal range in 9 of the cases.

  11. Production of second antibody for insulin and related peptides radioimmunoassay (RIA) (sheep anti-serum and guinea pig anti-IgG); Producao de segundo anticorpo para radioimunoensaio (RIE) de insulina e peptideos relacionados (antissoro de carneiro anti-IgG de cobaia)

    Energy Technology Data Exchange (ETDEWEB)

    Castanheira, Maria do Carmo; Silva, Sandra Rosa da; Borghi, Vania Caira [Instituto de Pesquisas Energeticas e Nucleares (IPEN), Sao Paulo, SP (Brazil); Wajchenberg, Bernardo Leo [Sao Paulo Univ., SP (Brazil). Faculdade de Medicina

    1995-12-31

    A good RIA separation technique is essential to develop precise assays and the double antibody separation method is one of the most widely employed, satisfying the majority of the criteria required by RIA. However, its high cost is its main disadvantage, which leads to employ less expensive techniques, that are not so efficient. Therefore, our institution is producing a second antibody to be used in insulin assays, in which the first antibody is raised in guinea pig. Three sheep were immunized with 500 {mu}g of guinea pig IgG purified at our laboratory emulsified in Freund Complete Adjuvante and administered by multisite subcutaneous injection at 20 day intervals. Blood samples were taken from the jugular vein 10 days after boosts. After each four boosts a great bleeding was done by the same route. After these bleeding, the animals were subjected to a rest before being reimmunized. The antisera title were determined by the immuno diffusion method in comparison with a reference antiserum of know quality produced in goat by the Pel-Freez, USA. Approximately 3.5 L of antiserum were produced from the three sheep which presented title very similar to those exhibited by the commercial product, even presenting higher values. (author). 8 refs., 3 figs.

  12. A more sensitive radioimmunoassay for neuron-specific enolase suitable for cerebrospinal fluid determinations.

    Science.gov (United States)

    Parma, A M; Marangos, P J; Goodwin, F K

    1981-03-01

    Neuron-specific enolase (NSE) and non-neuronal enolase (NNE) have been shown to be highly specific neuronal and glial products respectively and are therefore useful as biochemical markers of the two major cell types in the vertebrate central nervous system. An iodinated radioimmunoassay (RIA) procedure for human NSE (NSE-H) with approximately 50-fold greater sensitivity than the previously available tritiated assay is described. This assay is capable of detecting 100 pg of NSE-H per assay. NSE levels in human cerebrospinal fluid (CSF) which were previously undetectable with the tritiated RIA are now easily measured and have been shown to be approximately 2 ng/ml of CSF. Furthermore, results obtained with the newly described assay procedure on more concentrated brain tissue extracts are comparable to the tritiated RIA. The iodinated NSE RIA is also shown to be capable of accurately detecting added amounts of NSE in human CSF, indicating the potential clinical usefulness of this assay in determining elevated levels of NSE in CSF.

  13. Analysis of an iodide radioimmunoassay for 11-deoxicortisol measurement; Analise de um radioimunoensaio iodado para determinacao de 11-deoxicortisol

    Energy Technology Data Exchange (ETDEWEB)

    Madeira, Joao Luiz de Oliveira; Bussmann, Luciane Zgoda; Lima-Valassi, Helena Panteliou; Mendonca, Berenice Bilharinho de, E-mail: joao.madeira@usp.br [Universidade de Sao Paulo (HCFM/USP), SP (Brazil). Hospital das Clinicas. Departamento de Clinica Medica

    2014-04-15

    Objective: our aim was to correlate 11-deoxycortisol levels obtained by two currently available techniques for 11-deoxycortisol measurement: radioimmunoassay, and high performance liquid chromatography followed by tandem mass spectrometry (MS/MS). The latter is the gold standard method for steroid hormone measurement. Materials and methods: we selected 88 samples and the results of these two methods were compared by Deming regression. Results: the analytical sensitivity of the RIA was 0.30 ng/mL, with inadequate linearity and inadequate precision profile (34% of the samples had a CV ≥ 20%). From the selected samples, 54 had measurable levels of 11-deoxycortisol in both methods and were used in the comparison. The comparison of RIA with LC-MS/MS showed an overestimation of the results by RIA. The correlation coefficient was 0.610; linear regression slope was 3.751; and the intercept was 0.145, indicating a poor correlation between the two methods. Conclusion: we concluded that 11-deoxycortisol measured by radioimmunoassay, despite a good analytical sensitivity, showed very low specificity, precluding its use as a reliable method for 11-deoxycortisol measurement. (author)

  14. Simultaneous measurement of arginine vasopressin and oxytocin in plasma and neurohypophysis by radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Landgraf, R. (Deutsche Hochschule fuer Koerperkultur, Leipzig (German Democratic Republic). Forschungsinstitut Koerperkultur und Sport)

    1981-12-01

    Arginine vasopressin (AVP) and oxytocin (OXT) were measured simultaneously in the same sample by specific and sensitive radioimmunoassays (RIAs). The antibodies used did not cross-react to a variety of analogs and related peptides. The extraction procedure using Vycor glass powder resulted in mean recoveries of 84.4% (AVP) and 64.6% (OXT). In both assays, the sensitivity was 1 to 2 pg/ml plasma. A preincubation procedure that depresses plasma levels of both AVP and OXT selectively, provided specific blank values for a given plasma sample. To confirm the validity of the RIAs, dehydration experiments were performed. In rats, the basal levels of plasma AVP and OXT (means: 2,63 pg/ml and 6.80 pg/ml, respectively) are increased significantly after 24 h, 48 h and 72 h of water deprivation. Relationships are presented between both neurohormones in the plasma and neurohypophyses of control and dehydrated animals. As shown in cows, a significant correlation exists between plasma AVP and plasma osmolality but not between plasma OXT and osmolality or plasma AVP and OXT. Basal levels as well as physiological changes in plasma and neurohypophyseal AVP and OXT can be measured by the RIAs described.

  15. Evaluation of extraction methods for progesterone determination in rabbit (Oryctolagus cuniculus) feces by radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Korndoerfer, Clotilde Maria; Meirelles, Cyro Ferreira; Bueno, Ives Claudio da Silva; Abdalla, Adibe Luiz [Centro de Energia Nuclear na Agricultura (CENA), Piracicaba, SP (Brazil). Secao de Ciencias Animais]. E-mail: cfmeirel@esalq.usp.br

    1998-07-01

    The purpose of this study was to find a practical procedure for the extraction of progesterone (P{sub 4}) from feces and to determine if the P4 plasma profiles during pregnancy were reflected in total fecal P4 of pregnant rabbits. The rabbit was used as model for the techniques. Plasma and feces were collected from 11 rabbits during a period of 42 days. Three different methods of P4 extraction were used. The total P4 was measured by solid-phase radioimmunoassay (RIA) with {sup 125} I-P4 as the tracer. Results suggested that it was possible to extract total P4 from rabbit feces with methanol and petroleum ether. Plasma and fecal P4 profiles were compared for both pregnant and ovariectomized rabbits. It was possible to differentiate total P4 extracted from day two through 28 after breeding (p<0.01). (author)

  16. Iodine-125 radioimmunoassay for the direct detection of benzodiazepines in blood and urine

    Energy Technology Data Exchange (ETDEWEB)

    Goddard, C.P.; Stead, A.H.; Mason, P.A.; Law, B.; Moffat, A.C.; McBrien, M.; Cosby, S.

    1986-05-01

    A radioimmunoassay (RIA) for the direct detection of benzodiazepines in blood and urine is described. It is based on a commercially available antiserum and an easily synthesised radio-iodinated derivative of clonazepam that allows the use of relatively simple gamma-counting procedures. The assay can detect low therapeutic levels of all of the benzodiazepines currently available in the UK in 50-..mu..l samples of blood and urine (1-50 ng ml/sup -1/, depending on the drug); no prior sample preparation is required. It is inexpensive, rapid, simple to perform and is broadly specific for the benzodiazepine class of drugs. The assay offers a most suitable means of screening large numbers of samples of forensic interest for the presence of the benzodiazepines.

  17. A radioimmunoassay for measurement of thyroxine sulfate

    Energy Technology Data Exchange (ETDEWEB)

    Chopra, I.J.; Santini, F.; Hurd, R.E.; Chua Teco, G.N. (Univ. of California Center for the Health Sciences, Los Angeles (United States))

    1993-01-01

    A highly sensitive, specific, and reproducible RIA has been developed to measure T[sub 4] sulfate (T[sub 4]S) in ethanol extracts of serum. rT[sub 3] sulfate (rT[sub 3]S) cross-reacted 7.1%, and T[sub 3]S cross-reacted 0.59% in the RIA; T[sub 4], T[sub 3], rT[sub 3] and 3,3[prime]-diiodothyronine cross-reacted 0.004% or less. The recovery of nonradioactive T[sub 4]S added to serum averaged 95%. The detection threshold of the RIA was 18 pmol/L. The coefficient of variation averaged 6.9% within an assay and 12% between assays. T[sub 4]S was bound by T[sub 4]-binding globulin and albumin in serum. The free fraction of T[sub 4]S in four normal sera averaged 0.06% compared to a value of 0.03% for T[sub 4] (P < 0.001). The serum concentration of T[sub 4]S was (mean [+-] SE) 19 [+-] 1.2 pmol/L in normal subjects, 33 [+-] 10 in hyperthyroid patients with Graves disease, 42 [+-] 15 in hypothyroid patients, 34 [+-] 6.9 in patients with systematic nonthyroidal illnesses, 21 [+-] 4.3 in pregnant women at 15-40 weeks gestation, and 245 [+-] 26 in cord blood sera of newborns; the value in the newborn was significantly different from normal (P < 0.001). Administration of sodium ipodate (Oragrafin; 3 g, orally) to hyperthyroid patients was associated with a transient increase in serum T[sub 4]S. The T[sub 4]S content of the thyroid gland was less than 1/4000th that of T[sub 4]. We conclude that (1) T[sub 4]S is a normal component of human serum, and its levels are markedly increased in newborn serum and amniotic fluid; and (2) the sulfation pathway plays an important role in the metabolism of T[sub 4] in man. 28 refs., 4 figs., 2 tabs.

  18. Detection of respiratory virus antigens in nasopharyngeal secretions from patients with acute respiratory disease by radio-immunoassay and tissue culture isolation.

    Science.gov (United States)

    Ehrlicher, L; Hoffmann, H G; Habermehl, K O

    1984-01-01

    An investigation was made of the sensitivity and specificity of four-layer radio-immunoassays (RIA) in the detection of adenovirus, respiratory syncytial virus, influenza virus types A and B, as well as para-influenza virus types 1, 2 and 3 from nasopharyngeal aspirates of 146 patients with acute respiratory disease. The sensitivity of RIA was comparable with that of tissue culture isolation if the total number of positives is considered. The difference may have been caused both by a higher efficiency of the RIA for detection of inactivated or non-cultivable agents and by a higher efficiency of tissue culture methods if the samples contained only small amounts of antigen. Differences between the two antigen detection systems were found in particular with respiratory syncytial virus and influenza B virus. At present, the use of tissue culture isolation together with RIA is the optimal routine laboratory procedure for the diagnosis of respiratory infections.

  19. Determination of the complement components C1q, C4 and C3 in serum and cerebrospinal fluid by radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Dujardin, B.C.G.; Roijers, A.F.M.; Driedijk, P.C.; Out, T.A.

    1985-06-25

    Non-competitive 2-site radioimmunoassays (RIA) for the determination of the complement proteins C1q, C4 and C3 in cerebrospinal fluid (CSF) are described. The quantitative results of the RIAs were the same as those obtained by other assay methods: radial immunodiffusion and turbidimetry and, in the case of C4, the haemolytic assay. The ratios (concentration in CSF)/(concentration in serum) of the complement proteins correlated poorly with that of albumin. In contrast, the ratio of IgG was significantly correlated with that of albumin. The ratios of the complement proteins were higher than might be expected on the basis of their molecular masses. This suggests that these proteins may be synthesized within the normal central nervous system. (Auth.). 20 refs.; 3 figs.; 3 tabs.

  20. Purification of toad (Bufo japonicus) gonadotropins and development of their homologous radioimmunoassays

    Energy Technology Data Exchange (ETDEWEB)

    Takada, Koji; Itoh, Masanori; Nishio, Hiroshi; Ishii, Susumu (Waseda Univ., Tokyo (Japan))

    1989-10-01

    We obtained three gonadotropin fractions with different electrophoretic mobilities named B1D, B3D and B5D from a glycoprotein fraction of toad (Bufo japonicus) pituitaries by cation exchange chromatography using the fast protein liquid chromatography (FPLC) system, chromatofocusing and gel filtration using the FPLC system. Gonadotropin activity was monitored by two radioreceptor assay (RRA) systems, one using bullfrog testis and bullfrog LH as the source of receptor and radioligand respectively, and the other using toad testis and bullfrog FSH respectively. Although, LH/FSH specificity was not complete in these RRAs, the fraction BID showed a higher potency in LH-RRA than in FSH-RRA, while B3D and B5D showed lower potencies in LH-RRA activity than in FSH-RRA. Furthermore, B1D had an activity to release androgen from the toad testis, while B3D and B5D had slight activities. All these fractions stimulated accumulation of cAMP in testis slices of the toad in vitro. These results suggest that B1D contains LH, and B3D and B5D contain FSH-like gonadotropin. SDS PAGE analysis in combination with immunoblot revealed that B1D was almost pure LH, but B3D seemed to be not homogeneous. Anti-B1D-serum and anti-B3D-serum were raised in rabbits, and radioimmunoassays (RIAs) for B1D and B3D were established. The cross reactivity of B3D and B5D in B1D-RIA was about 30% of B1D, while that of B1D in B3D-RIA was only 3% of B3D and B5D. These RIAs were sensitive enough to measure gonadotropins in plasma samples of Bufo japonicus. (author).

  1. Clinical usefulness of trypsin radioimmunoassay kit

    Energy Technology Data Exchange (ETDEWEB)

    Kanamori, Isao; Nakano, Satoshi; Hurukawa, Masakazu; Okumura, Yasuki; Higuchi, Chizuko; Yanase, Mikiko; Onogi, Michiteru

    1988-08-01

    From the clinical use of RIA-gnost trypsin kit, the following results were obtained. 1. Standard curve showed a steep and good curve was shown. 2. Incubation: The condition for the first incubation was set at the room temperature for 10-24 hours and that for the second incubation at the room temperature for 3-5 hours. With these settings, satisfactory results were obtained. 3. Reproducibility and recovery: The C.V. of the reproducibility and the recovery were considered superior, and the values were below 10 % and +-3 %, respectively. 4. Correlation between trypsin and serum elestase-1: An excellent positive correlation (coefficient of correlation r = 0.889) was shown. 5. Serum trypsin concentration of normal and pancreatic diseases: The normal range was from 100 to 500 ng/ml. Acute pancreatitis rose obviously. Diabetes mellitus and chronic pancreatitis was below 500 ng/ml and the pancreatic cancer showed a tendency to scatter in the range of 50-1 250 ng/ml. The above results indicated that serum trypsin can be easily measured with high precision by using this method. Thus the method is considered useful for the diagnosis of pancreatic diseases.

  2. Direct radioimmunoassay of 17. beta. -estradiol in ether extracts of bovine

    Energy Technology Data Exchange (ETDEWEB)

    Medina, M.B.

    Anabolic estrogens such as 17..beta..-estradiol or 17..beta..-estradiol benzoate are used to promote growth and increase feed efficiency in food-producing cattle. This paper describes a technique to produce a more specific antibody to 17..beta..-estradiol by intradermal immunization using microquantities of 6-(carboxymethyl)-17..beta..-estradiol oxime bovine serum albumin and the development of a radioimmunoassay (RIA) procedure to measure directly the amounts of 17..beta..-estradiol in ether extracts of bovine serum without using cleanup procedures. Results demonstrated that a specific and sensitive antibody was produced, and a titer of 1:10,000 was used in the RIA procedure. Antibody cross-reactivity with ..beta..-estradiol metabolites and other anabolic estrogens was negligible. The untreated bovine sera showed 0-24 pg of apparent 17..beta..-estradiol/mL, while 0-31 pg/mL total estrogens had been reported in the literature. This assay can measure 5-100 pg in 20-250..mu..L/sample. This method can be used before or immediately after slaughter to monitor the residual amounts of estradiol used in the treatment of cattle.

  3. Direct radio-immunoassay of renin substrate: effect of converting enzyme inhibition

    Energy Technology Data Exchange (ETDEWEB)

    Metsaerinne, Kaj; Rosenloef, Katarina; Groenhagen-Riska, Carola; Fyhrquist, Frej

    1988-02-01

    A direct radio-immunoassay (RIA) for renin substrate (RS) was compared to enzymatic (indirect) assay. In normal subjects, a significant, albeit weak, correlation between the methods was seen. In hypertensive patients with different levels of plasma renin activity (PRA), RS concentration measured by both assays increased with increasing PRA, and for patients with PRA > 10 ..mu..g AI/l/h, direct assay gave significantly higher RS values (55%), compared to the enzymatic assay, indicating consumption of RS by increasing plasma renin and production rate of RS with increasing PRA. In 11 patients with renovascular hypertension, treatment with angiotensin-converting enzyme (ACE) inhibitor, lisinopril, resulted in a significant increase in PRA, accompanied by a decrease in RS measured by enzymatic assay. No change in RS measured by direct RIA was noticed. The results suggest that ACE inhibition may not have an effect upon RS production and that its effect on plasma RS is limited to a reduction of intact RS measured by the enzymatic assay.

  4. 21 CFR 864.7695 - Platelet factor 4 radioimmunoassay.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Platelet factor 4 radioimmunoassay. 864.7695 Section 864.7695 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES.... This device measures platelet activiation, which may indicate a coagulation disorder, such as...

  5. Detection by radioimmunoassay and enzyme-linked immunosorbent assay of coronavirus antibodies in bovine serum and lacteal secretions.

    Science.gov (United States)

    Rodak, L; Babiuk, L A; Acres, S D

    1982-07-01

    The sensitivity of a radioimmunoassay (RIA), an enzyme-linked immunosorbent assay (ELISA), and a serum neutralization assay (SN) for detecting antibodies to bovine coronavirus in serum and colostrum were compared. Although there proved to be a good correlation among all three assays (r = 0.915 and 0.964 for RIA with SN and ELISA, respectively), RIA and ELISA proved to be at least 10 times more sensitive than neutralization tests. By using these techniques, it was possible to detect a time-dependent decrease in antibody levels in bovine colostrum after parturition. Using ELISA, we demonstrated that 12 of 12 herds in Saskatchewan, and 109 of 110 animals tested, and antibody to bovine coronavirus. There was no elevated antibody response in serum or lacteal secretions of cows vaccinated once or twice with a commercially available modified live rota-coronavirus vaccine. In addition to being more sensitive than SN, ELISA and RIA proved to have other advantages for measuring antibody levels to bovine coronavirus and therefore warrant wider use as tools in diagnostic virology.

  6. A rapid easy—to—perform solid phase digoxin radioimmunoassay

    Institute of Scientific and Technical Information of China (English)

    LiBin; ZhouMei-Ying; 等

    1998-01-01

    A solid-phase-radioimmunoassay(SPRIA) for the monitoring of blood digoxin level has been developed,in which a secondary antibody-coated polystyrene tubes are used.This noval method seems to be simple to use and only takes about an half hour.The standard curve is linear from 0.25to 4μg/L.The sensitivity of the detection is 0.1μg/L.Reproducibility studies with 3 control sera of 0.5-2.5μg/L give intraassay CV<5% and interassay CV<10%.The specimens are measured and compared with those of the conventional radioimmunoassay and the values are well correlated(r=0.96,Y=1.022X+0.04μg/L)。

  7. Random assay in radioimmunoassay: Feasibility and application compared with batch assay

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Jung Min; Lee, Hwan Hee; Park, Sohyun; Kim, Tae Sung; Kim, Seok Ki [Dept. of Nuclear MedicineNational Cancer Center, Goyang (Korea, Republic of)

    2016-12-15

    The batch assay has been conventionally used for radioimmunoassay (RIA) because of its technical robustness and practical convenience. However, it has limitations in terms of the relative lag of report time due to the necessity of multiple assays in a small number of samples compared with the random assay technique. In this study, we aimed to verify whether the random assay technique can be applied in RIA and is feasible in daily practice. The coefficients of variation (CVs) of eight standard curves within a single kit were calculated in a CA-125 immunoradiometric assay (IRMA) for the reference of the practically ideal CV of the CA-125 kit. Ten standard curves of 10 kits from 2 prospectively collected lots (pLot) and 85 standard curves of 85 kits from 3 retrospectively collected lots (Lot) were obtained. Additionally, the raw measurement data of both 170 control references and 1123 patients' sera were collected retrospectively between December 2015 and January 2016. A standard curve of the first kit of each lot was used as a master standard curve for a random assay. The CVs of inter-kits were analyzed in each lot, respectively. All raw measurements were normalized by decay and radioactivity. The CA-125 values from control samples and patients' sera were compared using the original batch assay and random assay. In standard curve analysis, the CVs of inter-kits in pLots and Lots were comparable to those within a single kit. The CVs from the random assay with normalization were similar to those from the batch assay in the control samples (CVs % of low/high concentration; Lot1 2.71/1.91, Lot2 2.35/1.83, Lot3 2.83/2.08 vs. Lot1 2.05/1.21, Lot2 1.66/1.48, Lot3 2.41/2.14). The ICCs between the batch assay and random assay using patients' sera were satisfactory (Lot1 1.00, Lot2 0.999, Lot3 1.00). The random assay technique could be successfully applied to the conventional CA-125 IRMA kits. The random assay showed strong agreement with the batch assay. The

  8. Synthesis of iodobuprenorphine for use in radioimmunoassay. [Analgesic

    Energy Technology Data Exchange (ETDEWEB)

    Debrabandere, L.; Boven, M. van; Daenens, P. (Leuven Univ. (Belgium). Lab. of Toxicology)

    1992-08-01

    The synthesis of [[sup 125]I]iodobuprenorphine is described. This compound has been used as a tracer molecule in the development of a new radioimmunoassay for buprenorphine. The parameters that effect the direct iodination of buprenorphine using sodium [[sup 127]I]iodide, in the presence of either chloramine-T or Iodo-Beads, were studied. The structure of iodobuprenorphine was confirmed by mass spectrometry. (author).

  9. Error in anti-DNA antibody radioimmunoassay after gallium scanning

    Energy Technology Data Exchange (ETDEWEB)

    Torretti, D.; Rooney, P.; Williams, G.; Decker, J.L.

    1977-03-01

    Significant interference with the accurate measurement of anti-DNA antibodies occurs after gallium-67 scanning. The observed effect is dependent on the radioimmunoassay used. False-negative results are observed with a modified Farr assay whereas false-positive results are noted in the millipore filter assay. These spurious values are the result of persistent radioactivity in the patients' sera after administration of /sup 67/Ga citrate.

  10. Radioimmunoassay in the evaluation of pancreatic function in chronic pancreatitis

    Energy Technology Data Exchange (ETDEWEB)

    Zubovskij, G.A.; Vasil' chenko, S.A. (Nauchno-Issledovatel' skij Inst. Rentgenologii i Radiologii, Moscow (USSR))

    1983-12-01

    Insular apparatus function in primary chronic and reactive pancreatitis associated with hepatobiliary pathology was studied in 178 patients by radioimmunoassay. Typical changes of insulin and C-peptide concentration in the presence of an intravenous glucose tolerance test are shown that make it possible to differentiate in combination with the trypsin concentration in the serum primary and reactive pancreatitis as well as exacerbation and remission stages of the disease.

  11. Comparison of enzyme-linked immunosorbent assay and radioimmunoassay for prostate-specific acid phosphatase in prostatic disease

    Energy Technology Data Exchange (ETDEWEB)

    Griffiths, J.; Rippe, D.F.; Panfili, P.R.

    1982-01-01

    Results of an enzyme-linked immunosorbent assay (ELISA) are compared with those of a standard radioimmunoassay (RIA) for detection and quantitation of prostate-specific acid phosphatase (EC 3.1.3.2) in serum. Control subjects, patients with benign prostatic hyperplasia, and patients in all four clinical stages of prostatic adenocarcinoma were tested. The upper limit of normal (95%of the population) by the ELISA was 2.0 ..mu..g/L, and by the RIA was 2.2 ..mu..g/L. In prostatic a denocarcinoma stage I (not detectable by digital rectal examination), ELISA was slightly more sensitive than RIA, but sensitivity was still relatively low (20%). As tumor mass increased (stages II through IV), the frequency of increased concentrations of prostatic acid phosphatase in serum also increased. We confirmed this increase in circulating enzyme in some cases of benign prostatic hyperplasia and suggest that this finding is related to either acinar cytolysis or an increase in acini size and number. Although prostate-specific acid phosphatase is not a cancer-specific enzyme, we conclude that its measurement may be of considerable value in monitoring prostatic disease.

  12. Interaction of thyroid hormone and hemoglobin: nature of the interaction and effect of hemoglobin on thyroid hormone radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Davis, P.J.; Yoshida, K.; Schoenl, M.

    1980-05-01

    Gel filtration of human erythrocyte (RBC) lysate incubated with labeled thyroxine (Tu) or triiodothyronine (Tt) revealed co-elution of a major iodothyronine-binding fraction (R-2) and hemoglobin. Solutions of purified human hemoglobin and Tt also showed co-elution of hormone and hemoglobin. Because hematin and protoporphyrin were shown to bind labeled Tt, the oxygen-binding site on hemoglobin was excluded as the site of iodothyronine-hemoglobin interaction. Analysis of hormone binding by heme and globin moieties showed Tt binding to be limited to the heme fraction. Addition of excess unlabeled Tt to hemoglobin or heme incubated with labeled Tt indicated 75% to 90% of hormone binding was poorly dissociable. These observations suggested that the presence of hemoglobin in RBC lysate or in serum could influence the measurement of Tu and Tt by specific radioimmunoassay (RIA). Subsequent studies of the addition to serum of human hemoglobin revealed a significant reduction in Tt and Tu detectable by RIA in the presence of this protein. The effect was influenced by the concentration of hemoglobin and by duration and temperature of incubations of hemoglobin and serum prior to RIA.

  13. Radioimmunoassay of serum follicle-stimulating hormone and luteinizing hormone in the bottlenosed dolphin.

    Science.gov (United States)

    Schneyer, A; Castro, A; Odell, D

    1985-11-01

    Commercially available radioimmunoassay (RIA) kits for human follicle-stimulating hormone (FSH) and luteinizing hormone (LH) were adapted for quantitation of these hormones in serum from bottlenosed dolphins (Tursiops truncatus). Serum samples from over 160 wild and 70 captive animals were assayed in order to determine basal concentrations of FSH and LH in these animals, as well as to detect possible differences between various groups. Mean FSH and LH levels for all animals were 0.22 +/- 0.08 and 0.37 +/- 0.18 ng/ml, respectively. Although wild animals had higher FSH and LH levels than captive ones, the differences were not statistically significant (P less than 0.07). However, both FSH and LH were significantly (P less than 0.01 and P less than 0.05, respectively) elevated in females when compared to males. Adults and peripubescent animals had significantly (P less than 0.01) higher LH levels than did juveniles. Among wild animals, serum concentrations of FSH and LH reflected seasonal differences. Samples obtained in early summer (Gulf of Mexico population) contained significantly (P less than 0.01) higher concentrations of FSH and LH than samples obtained in the fall (Indian River, Florida population). Both FSH and LH were significantly elevated in samples from confirmed pregnant animals as compared to the overall mean and to a sample from a confirmed nonpregnant female. Our observations indicate that these RIAs can reliably detect serum FSH and LH from bottlenosed dolphins and represent the first quantitation of these hormones in cetaceans.

  14. Radioimmunoassay of bovine, ovine and porcine luteinizing hormone with a monoclonal antibody and a human tracer

    Energy Technology Data Exchange (ETDEWEB)

    Fosberg, M.; Tagle, R.; Madej, A.; Molina, J.R.; Carlsson, M.-A.

    1993-01-01

    A radioimmunoassay for bovine (bLH), ovine (oLH) and porcine (pLH) luteinizing hormone was developed using a human [sup 125]ILH tracer from a commercial kit and a monoclonal antibody (518B7) specific for LH but with low species specificity. Standard curves demonstrated similar binding kinetics when bLH, oLH and pLH were incubated with tracer and antibody for 2 h at room temperature. A 30-min delay in the addition of the tracer gave sufficient sensitivity when analysing pLH. Separation of antibody-bound LH from free hormone was achieved by using second antibody-coated micro Sepharose beads. The assay was validated and the performance compared with that of an RIA currently in use for determination of bLH (coefficient of correlation: 0.99 and 0.98). Regardless of the standards used, intra-assay coefficients of variation were <10% for LH concentrations exceeding 1 [mu]g/L. The inter-assay coefficients of variation were <15%. The assay was used for clinical evaluation demonstrating the pre-ovulatory LH surge in two cyclic cows, LH pulsatility in an oophorectomized ewe and LH response to GnRH injection in a boar. (au) (7 refs.).

  15. Homologous radioimmunoassay for human epidermal growth factor (urogastrone)

    Energy Technology Data Exchange (ETDEWEB)

    Dailey, G.E.; Kraus, J.W.; Orth, D.N.

    1978-06-01

    Epidermal growth factor (EGF), a polypeptide hormone originally discovered in the mouse submaxillary gland, stimulates growth in a variety of tissues in several species. This hormone has recently been identified in human urine. A homologous RIA for human EGF (RIA-hEGF) has been developed. In general, levels were similar to those recently reported using a heterologous RIA system. Twenty-four-hour urinary excretion of RIA-hEGF by normal adult males and females was 63.0 +- 3.0 and 52.0 +- 3.5 (mean +- SE) ..mu..g/total vol, or 29.7 +- 1.1 and 39.8 +- 1.7 ..mu..g/g creatinine, respectively. Excretion by females taking oral contraceptives was significantly greater (60.1 +- 2.7 ..mu..g/g creatinine; P < 0.01) than that by females who were not. Recent evidence suggests the probable identity of hEGF and ..beta..-urogastrone, a potent inhibitor of gastric acid secretion. Adult males with active peptic ulcer disease appeared to have lower urinary RIA-hEGF excretion (22.9 +- 2.6 ..mu..g/g creatinine) than normal men, but this was not significant (P > 0.05). Several of those with very low values had histories of alcohol abuse. Excretion by patients with Cushing's syndrome was normal. Patients with psoriasis or recovering from major burns excreted both abnormally high and abnormally low levels of RIA-hEGF, with no obvious correlation to their clinical condition. There was no apparent diurnal or postprandial variation in urinary RIA-hEGF excretion by normal subjects. An excellent linear correlation was observed between RIA-hEGF and creatinine concentrations in each urine sample for each subject, suggesting that RIA-hEGF concentration in a random urine sample provides a valid index of 24-h RIA-hEGF excretion.

  16. Radioimmunoassay for prostatic acid phosphatase in human serum. Methodologic aspects

    Energy Technology Data Exchange (ETDEWEB)

    Pradalier, N.; Canal, P.; Pujol, A.; Fregevu, Y. (Groupe de Recherches du Centre Claudius-Regaud, Toulouse (France)); Soula, G. (Faculte des Sciences Pharmaceutiques, Toulouse (France))

    1982-01-01

    We propose a double antibody radioimmunoassay for human prostatic acid phosphatase (PAP) in serum for diagnosis and management of prostatic adenocarcinoma under treatment. The antigen is purified from human prostatic fluid by a gel-filtration on Sephadex G 100 followed by affinity chromatography on Con A Sepharose. A specific antibody is raised in rabbits and purified by immunoadsorption with a female serum. The described technique offers both radioisotopic sensibility and immunologic specificity. Physiological values determined in the serum of 125 healthy males are below 2 ng/ml. No significative differences are observed with age. The proposed technique also shows significant differences between values evaluated for benign prostatic hyperplasia and prostatic adenocarcinoma.

  17. Prediction of ovulation in women using a rapid progesterone radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Fleming, R.; Coults, J.R.T. (Glasgow Univ. (UK))

    1982-02-01

    A rapid (3-h) radioimmunoassay of plasma progesterone has been developed and used successfully to predict the time of ovulation in women undergoing artificial insemination. The results obtained using progesterone levels to date the stage of the cycle were analysed retrospectively by (1) estimation of the length of the ensuing luteal phases and comparison of these with luteal phase lengths of a control group (2) comparison of the dating using progesterone levels with retrospective determination of LH values and (3) by analysis of the dating in cycles in which conception occurred.

  18. Use of radioimmunoassay to diagnose some forms of endocrine sterility

    Energy Technology Data Exchange (ETDEWEB)

    Balabolkin, M.I.; Gerasimov, G.A. (Moskovskij Meditsinskij Stomatologicheskij Inst. (USSR))

    1984-11-01

    The possibility is considered of using a metoclopramide test combined with radioimmunoassay for determining prolactin in the blood to diagnose some forms of endocrine sterility of 17 women examined 5 are practically healthy, sterility of 6 women is caused by tumor of hypophysis verified by X-rayograms, 6 women suffered from disorderded prolactin secretion and sterility without roentgenologic signs of disordered sella turcica. Metoclopramide has been injected intravenously in the 10 mm dose. A series of advantages is noted of the metoclopramide sample characteristic of high availability of the preparation and practically complete absence of side-effects.

  19. A radioimmunoassay for alpha- and beta-gliadins.

    Science.gov (United States)

    Ciclitira, P J; Lennox, E S

    1983-06-01

    1. A rapid, sensitive specific radioimmunoassay for alpha- and beta-gliadin has been developed using an antiserum raised in rabbits to A-gliadin, a component of alpha-gliadin. 2. The antigen used in the assay was alpha-gliadin labelled with 125I; antigen-antibody complexes were collected after adsorption to Staphylococcus aureus in suspension. 3. The sensitivity of the assay, as judged by competitive binding with unlabelled antigen, was 1 ng of alpha- or beta-gliadin, which show complete cross-reaction with this antiserum. 4. Cross-reactivity to other wheat proteins was less than 1% and no cross-reactivity to extracts of rye, barley or oats was observed. 5. This radioimmunoassay for alpha- and beta-gliadin has been used to measure their amount in different varieties of wheat flour, several foods prepared from flour, e.g. bread, biscuits and products prepared as 'gluten free'. The possibility of assaying for alpha-gliadin in prepared foods is of special value since alpha-, beta-, gamma- and omega-gliadin have been shown to exacerbate coeliac disease.

  20. Stereospecific radioimmunoassay of alpha-l-acetylmethadol (LAAM).

    Science.gov (United States)

    McGilliard, K L; Olsen, G D

    1980-10-01

    A sensitive and specific radioimmunoassay has been developed which allows quantitation of picogram amounts of alpha-l-acetylmethadol (LAAM) in biological fluids without prior extraction. Antiserum was obtained from rabbits immunized with antigen prepared by conjugation of alpha-l-methadol hemisuccinate with thyroglobulin. The antiserum was stereospecific, distinguishing LAAM from its three stereoisomers. In addition, there was little cross-reaction in the assay by metabolites of LAAM and no interference with the assay by other narcotic analgesics. The sensitivity of the assay was 50 pg, requiring serum sample sizes as small as 10 microliters. The radioimmunoassay was used to determine serum concentrations of LAAM in five female dogs receiving i.v. injections of 2 mg/kg of LAAM. The decay of LAAM in serum appeared to be multiexponential, with a mean terminal half-life of 35 hr. The dogs were behaviorally depressed for at least 9 hr and hypothermic for at least 24 hr after injection. The persistence of these pharmacological effects was probably due to the slow elimination of LAAM and the presence of major metabolites which are pharmacologically active.

  1. [A sensitive and specific radioimmunoassay for arginine vasopressin and its validation].

    Science.gov (United States)

    Oki, Y; Ohgo, S; Yoshimi, T

    1984-03-20

    A sensitive and specific radioimmunoassay (RIA) for arginine vasopressin (AVP) has been developed and validated. Synthetic AVP was coupled to bovine serum albumin (BSA) with glutaraldehyde. Antisera against AVP were raised in three rabbits immunized with AVP-BSA complex. After 6 months, at the 16th injection, one of the antisera had a titer high enough to be utilizable for RIA at a final dilution of 1:400,000. The labeling of AVP with 125I Na was performed with the modified chloramine T method, and the purification of iodinated AVP was done with gel filtration chromatography on a Sephadex G-25 fine column (1 X 20 cm) with an elution buffer of 0.01 M acetic acid containing 0.1% BSA. Radioactivities from the Sephadex G-25 were eluted in three peaks. 125I-AVP, which was reactive to the antiserum, was contained in the third peak, and 125I-AVP in the fractions on the down slope of the peak was used for the radioligand in the amount of 1000 cpm. The specific activity of purified 125I-AVP was about 400 muCi/microgram. Diluted antiserum and samples, unlabeled AVP or related peptides were preincubated at 4 degrees C for 24 hr, and then 125I-AVP was added to the mixture and incubated for a further 72 hr. Separation of B and F was done with polyethyleneglycol. The minimal detection limit of AVP, which was 95% of the confidence limit of the mean value of B0, was 0.4 pg/tube. The cross-reactivities with lysine vasopressin, arginine vasotocin, DDAVP and oxytocin were 0.1%, 30%, 1% and 0%, respectively. AVP in plasma was extracted with cold acetone and petroleum ether. The recoveries of synthetic AVP from plasma which was added (2-16 pg) were more than 94%. The intra and inter-assay coefficients of variation determined by plasma of AVP concentration of about 4.8 pg/ml were 8.7% and 11.3%, respectively. The RIA detected AVP of concentration as low as 1 pg/ml following the extraction procedure. AVP immunoreactivity was detected without extraction in urine, and the lyophilized

  2. Radioimmunoassay in the evaluation of the hormonal activity of hypophyseal adenoma

    Energy Technology Data Exchange (ETDEWEB)

    Snigireva, R.Ya.; Kasumova, S.Yu.; Barabanov, V.M. (Akademiya Meditsinskikh Nauk SSSR, Moscow. Inst. Nejrokhirurgii; Akademiya Meditsinskikh Nauk SSSR, Moscow. Inst. Morfologii Cheloveka)

    1983-08-01

    Direct correlation was found between the results of a radioimmunoassay electron microscopy and immunohistochemistry in cases of STH-secreting and hormonally inactive hypophyseal adenomas. In hyperprolactinemia and a simultaneous increase in the levels of 2 or more hormones the results of the radioimmunoassay coincided with those of electron microscopy and immunohistochemistry in 50-70% of the cases.

  3. Development of a new radioimmunoassay for erythropoietin using recombinant erythropoietin

    Energy Technology Data Exchange (ETDEWEB)

    Mason-Garcia, M.; Beckman, B.S.; Brookins, J.W.; Powell, J.S.; Lanham, W.; Blaisdell, S.; Keay, L.; Li, S.C.; Fisher, J.W. (Tulane Univ. School of Medicine, New Orleans, LA (USA))

    1990-11-01

    The development of a 24 hour radioimmunoassay for erythropoietin (EPO) using EPO derived from recombinant DNA as both immunogen and ligand is described in the present paper. Mixed breed rabbits immunized with 10 micrograms/kg of EPO derived from a stably transfected cell line (MD) produced antibodies to EPO with high titer (up to 1:896,000 final dilution in the tube), high affinity (8.4 x 10(11) liter/M), and good specificity. Purified EPO from the above source or from AmGen Biologicals (AG) were successfully radioiodinated with the chloramine-T method and used as ligand in the radioimmunoassay. Standard dose-response curves prepared with EPO from both commercial sources were not significantly different and showed a sensitivity of 0.75 to 0.96 mU/tube. The dose-response curves in both systems also showed parallelism with serially diluted serum from a patient with aplastic anemia. Within-assay and between-assay precision were determined by assaying multiple replicates of a serum pool. Recovery of exogenous EPO added to a serum pool averaged 97% for both systems. The range of normal human serum EPO was determined by assaying the sera of 153 hematologically-normal adult subjects and was found to be 1.1 to 27.3 mU/ml for MD EPO and 0.5 to 16.7 mU/ml for AG EPO. Sera from several patients with hematologic abnormalities were also assayed, including those of 36 patients with anemia of end-stage renal disease (mean +/- SEM, 29.5 +/- 4.0 mU/ml; P less than 0.01). In conclusion, this new, more rapid and sensitive radioimmunoassay system can be used to measure EPO levels in sera from normal human subjects and patients with several types of anemia, and should also be very useful in therapeutic drug monitoring of patients receiving EPO from various commercial sources.

  4. Radioimmunoassay of fragment E-related neoantigen: validation studies and clinical application. [Fibrinogen-fibrin degradation products

    Energy Technology Data Exchange (ETDEWEB)

    Chen, J.P.; Hanna, W.T.; Williams, T.K.; Krauss, S. (Tennessee Univ., Knoxville (USA). Memorial Research Center and Hospital)

    1984-05-01

    An E-neoantigen radioimmunoassay (Eneo RIA) is described which can determine normal and pathological plasma levels of E-related fibrinogen-fibrin degradation products (FDP). The assay employs rabbit antiserum produced against fragment E derived from a plasmin digest of fibrinogen and subsequently absorbed with fibrinogen. The absorbed antiserum contains antibodies which are equally reactive with fibrinogen derived E (Fg-E) and fibrin derived E(Fb-E) but not with fibrinogen at 1 mg/ml. The Eneo RIA was validated by assay parallelism and by recovery experiments. Plasma Eneo immunoreactivities in 14 normals were 4-22 ng/ml (mean 12.7 ng/ml). Plasma Eneo levels in 23 of 24 patients with neoplastic and haematological diseases were elevated above normal (range 27-2027 ng/ml). Unusually high Eneo values were observed with three patients whose diseases were complicated by either disseminated intravascular coagulation (DIC) or deep vein thrombosis. After heparin therapy, the Eneo level of a patient with chronic DIC declined. A pathological plasma was eluted from a Sephadex G-200 column and Eneo immunoreactivity was determined on the eluates. The gel filtration pattern of Eneo indicates that E-related FDP is a family of plasmic fragments derived from crosslinked fibrin.

  5. [Radioimmunoassay for human plasma 8-arginine-vasopressin (author's transl)].

    Science.gov (United States)

    Conte-Devolx, B; Rougon-Rapuzzi, G; Millet, Y

    1977-01-01

    The authors have developed a radioimmunoassay for human plasma vasopressin (AVP) which permits the estimation of antidiuretic hormon (ADH) levels as low as 0,8 pg/ml. The average plasma level of AVP after overnight water restriction was found to be 14,3 pg/ml (sd = 4,4 pg/ml) in normal subjects. They provoked a hypersecretion of ADH by the intravenous injection of 1-2 mg of nicotine. In 11 volunteer normal subjects this stimulation by nicotine provoked ADH hypersecretion which reached a maximum between 2nd and 15th minutes after injection. In 3 cases of diabetes insipidus, nicotine injection did not induce ADH hypersecretion; in 1 case of potomania this response was weak; in 2 cases of syndrome of inappropriate ADH secretion, AVP plasma levels were elevated and the response after nicotine stimulation was exaggerated.

  6. Radioimmunoassay for pregnancy-associated plasma protein A

    Energy Technology Data Exchange (ETDEWEB)

    Sinosich, M.J. (Royal North Shore Hospital, Sidney, New South Wales, Australia); Teisner, B.; Folkerson, J.; Saunders, D.M.; Grudzinskas, J.G.

    1982-01-01

    A specific and highly sensitive radioimmunoassay for determination of pregnancy-associated plasma protein A in human serum is described. The minimum detection limit for this protein was 2.9 ..mu..g/L. The within- and between-assay coefficients of variation were 4.0 and 4.5%, respectively. The circulating protein was detected within 32 days of conception in eight normal pregnancies and within 21 days in a twin pregnancy. Circulating concentrations in the mother at term were consistently higher (10-fold) than in matched amniotic fluid; none was detected in the umbilical circulation. This protein was also detected in the circulation of patients with hydatidiform mole. This assay will permit investigations into the clinical evaluation of measurements of the protein during early pregnancy and trophoblastic disease.

  7. Radioimmunoassay of hair for determining opiate-abuse histories

    Energy Technology Data Exchange (ETDEWEB)

    Baumgartner, A.M.; Jones, P.F.; Baumgartner, W.A.; Black, C.T.

    1979-07-01

    Heroin and morphine metabolites can be detected in hair with the use of commerically available radioimmunoassay reagents and with minor sample preparation. Hair samples obtained from morphine-treated mice and heroin users contained nanogram levels of the drug per milligram of hair (single human hair). The results of the hair analyses for all subjects admitting the use of heroin were positive, whereas the results of only 30% of thin-layer chromatographic urinanalyses of these same subjects were positive. In addition, differences in drug concentration for sections of hair near the scalp and near the distal end correlated with the length of time the drug had been used. These results exemplify the potential advantages of the use of hair analysis over urine and serum analyses in terms of accessibility, sample stability, and long-term retention of information.

  8. Radioimmunoassay of human placental protein 14 (PP14)

    Energy Technology Data Exchange (ETDEWEB)

    Bolton, A.E.; Stoker, R.J. (North East London Polytechnic (UK)); Chapman, M.G.; Wass, D. (Queen Charlotte' s Maternity Hospital, London (UK)); Andrew, C.E. (Edgware General Hospital (UK)); Bohn, H. (Behringwerke AG, Marburg/Lahn (Germany, F.R.). Research Labs.)

    1983-12-30

    The development and validation of a radioimmunoassay for the measurement of human placental protein 14 in maternal serum is described. The mean concentration of this protein in serum from 22 normal pregnant women showed a decline during the third trimester from 120 ..mu..g/l at 27 weeks gestation to 65 ..mu..g/l at term. Serum samples from 16 patients with intra-uterine growth retardation tended to contain lower concentrations of placental protein 14, these results reaching significance at weeks 36-38 of gestation. Of seven patients with pre-eclampsia from whom two or more blood samples were taken, four showed increases in concentration of this protein as pregnancy proceeded, compared with the normal pattern of decreasing values.

  9. Radioimmunoassay of aspartate aminotransferase isoenzymes in human serum

    Energy Technology Data Exchange (ETDEWEB)

    Leung, F.Y.; Niblock, A.E.; Henderson, A.R.

    1984-08-01

    A description is given of the development of a sensitive, specific radioimmunoassay for the cytoplasmic and mitochondrial isoenzymes of human aspartate aminotransferase (L-aspartate:2-oxoglutarate aminotransferase; EC 2.6.1.1). Isoenzymes from human heart tissue were purified to homogeneity and used to raise high-titer antisera in rabbits. The antisera were partly purified by selective column chromatography. The Bolton-Hunter reagent was used to radioiodinate the isoenzymes. The assay requires 100 microL of serum, includes a solid-phase second-antibody separation, and can be completed in less than 3 h. There was no cross reactivity between the two isoenzymes. As little as 5 micrograms (50 pmol) of each aspartate aminotransferase can be measured per liter of serum.

  10. A radioimmunoassay for rat ghrelin: evaluation of method and effects of nonylphenol on ghrelin secretion in force-fed young rats.

    Science.gov (United States)

    Chang, Yen-Jui; Huang, Wei-Ju; Lin, Han-Wei; Chang, Ling-Ling; Chang, Full-Young; Wang, Paulus S

    2011-10-31

    Antiserum YJC 13-31 against the rat ghrelin conjugated to bovine serum albumin (BSA) was produced in the rabbit and a double antibody radioimmunoassay (RIA) for ghrelin has been developed. Characterization results of this antiserum revealed no cross-reaction with human growth hormone and somatostatin. Weak cross-reactions with insulin (0.1%), rat growth hormone (0.1%) and glucagon (0.3%) were observed, which scarcely interfered the assay system. The sensitivity of this RIA was 5 pg per assay tube. With the rat serum samples, the within-assay precision was 7.1% and the between-assay precision was 12.3%. The RIA was also available to detect the ghrelin in rat tissue extracts with good parallelism to the rat ghrelin standard. In application, the serum ghrelin and corticosterone levels in weaned rats were measured by RIA. Gavage of saline was sufficient to raise serum ghrelin from 2.6 +/- 0.18 to 6.7 +/- 0.7 ng/ml (P ghrelin levels in a dose-dependent manner. Besides, gavages of saline elevated the serum levels of corticosterone from 108.8 +/- 13.5 to 188.7 +/- 23.5 ng/ml (P < 0.01) but the elevation effects of corticosterone from gavages were overcome by NP in the low dose of 50 mg/kg. It can be speculated that ingestion of NP is harmful to young animals during growth and environmental adaptation.

  11. Determination of femtomol quantities of gibberellic acid by radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Weiler, E.W.; Wieczorek, U.

    1981-01-01

    A highly sensitive and specific radioimmunoassay which allows the detection of as little as 5 fmol (2 pg) of gibberellic acid (GA/sub 3/) in crude plant extracts is described. Antisera of high affinity and titer were obtained by immunizing rabbits with a conjugate of carboxyl-coupled GA/sub 3/ and bovine serum albumin. (/sup 125/I) Gibberellic acid-(N-(p-hydroxybenzyl) putrescine) amide of high specific activity, used as the immunotracer, is readily displaced by gibberellic acid methyl ester but not by free gibberellic acid. Thus, methylation of extracts prior to analysis is required. The assay is very specific; besides GA/sub 3/, only the closely related GA/sub 7/ is highly immunoreactive. Various gibberellins, related compounds, as well as other classes of plant hormones do not interfere with the assay. Levels of immunoreactive gibberellins (GA/sub 3/, GA/sub 7/) in actively growing tissues, among them cell suspension cultures of 33 different species, were determined.

  12. Radioimmunoassay in the diagnosis of atypical form of thyrotoxicosis

    Energy Technology Data Exchange (ETDEWEB)

    Livshits, G.Ya.

    1984-11-01

    Fifty-six patients with ''unmotivated'' disorder of the cardiac rhythm were examined. A combined radionuclide study including a study of iodoabsorptive function with a standard technique, thyroid visualization and determination of the thyroxin, triiodothyronine level in the blood serum with the radioimmunoassay using standard diagnostic kits, was conducted, Latent thyroid hyperfunction was revealed in 24 patients (42.8%). Study of iodoabsorptive function revealed pathological changes in 8 patients only, whereas raaioimmunoassay revealed a significant elevation of the peripheral thyroid hormone level as compared to that of the control group in 24 patients. The conclusion is that patients with ''unmotivated'' disorder of the cardiac rhythm often suffer from latent thyrotoxicosis which is the main etiological factor and trigger mechanism of arrhythmias. In such a situation they are the only clinical symptom of thyrotoxicosis that makes it possible to regard this form of disease as monosymptomatic. The early detection of the cause of cardiac rhythm disorder and the prescription of pathogenetic thyrostatic therapy resulted in the return of the cardiac cycle rate to normal in all the patients with sinus tachycardia and prevented relapses of the paroxysmal forms of rhythm disorder.

  13. Radioimmunoassay of calcitonin in unextracted human serum: a sensitive method

    Energy Technology Data Exchange (ETDEWEB)

    Emmertsen, K.; Marqversen, J.; Jensen, F.T.; Hansen, H.H. (Kommunehospitalet, Copenhagen (Denmark))

    1982-11-01

    A sensitive sequential direct radioimmunoassay of calcitonin (CT) is described. Human synthetic monomer CT was used for /sup 125/I-labelling. The antibody was directed against mid-portion and C-terminal parts of the human CT molecule. No significant cross reactivity with salmon or porcine CT was found. Separation of free from antibody-bound /sup 125/I-labelled CT was performed using a double antibody-polyethyleneglycol mixture. Dilution curves of sera from normal controls and patients with medullary thyroid carcinoma (MCT) were parallel to the standard curve. The intraassay coefficients of variation (CV) were 6% and 3% at serum concentrations of 90 and 230 ng/l respectively. The interassay CV were 13% and 10% at mean serum CT concentrations of 50 and 210 ng/l respectively. The lower limit of detection was 20 ng/l. Thirty-one healthy controls had serum CT levels in the range of 35-135 ng/l. Elevated basal serum CT concentrations were found in 11 patients with MCT and the serum concentrations increased markedly after pentagastrin stimulation. Subsequent thyroidectomy in eight of the 11 patients with MCT reduced basal serum CT levels, in six to within the reference range for normals.

  14. Beta 2-Microglobulin clearance as measured by radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Woo, J.; Floyd, M.; Longley, M.A.; Cannon, D.C.

    1980-07-01

    We describe a radioimmunoassay for beta 2-microglobulin (beta 2 mu) in serum and urine. We incubated aliquots of diluted samples at room temperature for 1 h with /sup 125/I-labeled beta 2 mu and a rabbit antiserum monospecific for human beta 2 mu, and separated the phases by the double-antibody technique. The logit-log transformed dose-response curve was linear in the range 2 to 64 ng, equivalent to 0.5 to 16 mg/L of serum and 0.5 to 320 mg/L of urine. Assay sensitivity was 2.4 ng of beta 2 mu. Validation studies included tests of precision, accuracy, antibody specificity, and parallelism of the dose-response curves for standard and unknown. In a study of 25 normal individuals, serum and urine beta 2 mu ranged from 1.1 to 2.3 mg/L and 40 to 360 micrograms/24 h; the clearance of beta 2 mu was 8 to 130 microL/min. In 21 renal allograft recipients tested one to five weeks after transplantation, serum and urine beta 2 mu ranged from 3.9 to 15.6 mg/L and 7.2 to 611 mg/24 h; beta 2 mu clearance was 0.60 to 33.3 mL/min. Values for both serum and urine correlated well with severity of allograft rejection.

  15. Radioimmunoassay for immunolobulin G in serum and urine

    Energy Technology Data Exchange (ETDEWEB)

    Woo, J.; Floyd, M.; Longley, M.A.; Cannon, D.C.

    1979-12-01

    We describe a radioimmunoassay for immunoglobulin G (IgG) in serum and urine. Aliquots of diluted samples and /sup 125/I-labeled IgG were incubated in antibody-coated tubes at 37 degrees C for 24 h, the supernates were decanted, and the radioactivity in tubes containing the bound fraction was counted. The dose-response curve in the range of 0.4-500 mg/L of urine or 640-40 000 mg/L of serum was linear on logit-log transformation and iterative weighted regression. Assay sensitivity was 10 ng of IgG. Validation studies included testing for precision, accuracy, antibody specificity, and parallelism of the dose-response curves for standard and unknown. In a study of 14 apparently normal individuals, serum IgG = 4.0-10.9 gL, urine IgG = 1.1-4.8 mg/24 h, and IgG clearance = 0.2 x 10/sup -4/ to 4.8 x 10/sup -4/ mL/min. In 20 patients with renal allografts, serum IgG = 15.8-66 g/L, urine IgG = 9.6-626 mg/24 h, and IgG clearance = 9 x 10/sup -4/ to 1.99 x 10/sup -1/ mL/min. IgG values correlate well with severity of renal allograft rejection.

  16. Radioimmunoassay for detection of VP1 specific neutralizing antibodies of foot and mouse disease virus

    Energy Technology Data Exchange (ETDEWEB)

    Patzer, E.J.; Jackson, M.L. (Genentech, Inc., South San Francisco CA (USA)); Moore, D.M. (U.S. Department of Agriculture, Plum Island Animal Disease Center, Greenport, NY (USA))

    1985-01-01

    A solid-phase radioimmunoassay was developed for the detection of antibodies against a specific region of the VP1 protein of the A24 and O1 serotypes of foot and mouth disease virus. The antibody titers from the radioimmunoassay showed a positive correlation with neutralizing antibody titers determined by a mouse protection assay. The specificity of the assay resides in the peptide used as antigen. The assay is rapid, reproducible and does not require the use of whole virions.

  17. Development of a C-terminal-region-specific radioimmunoassay of parathyroid hormone-related protein

    Energy Technology Data Exchange (ETDEWEB)

    Kasahara, Hiroyuki; Tsuchiya, Masumi; Adachi, Ryoji; Horikawa, Shuji; Tanaka, Shuichi; Tachibana, Seiji (Daiichi Radioisotope Labs. Ltd., Tokyo (Japan))

    1992-04-01

    Few data are published regarding the molecular forms or concentrations of circulating and urinary parathyroid hormone-related protein (PTHrP) in normal subjects and patients with humoral hypercalcemia of malignancy (HHM). We have developed a C-terminal-region-specific radioimmunoassay for human PTHrP 109-141 (C-PTHrP radioimmunoassay) using a sheep antiserum immunized with a novel synthetic human PTHrP 109-141 for immunogen and a novel synthetic [Tyr[sup 108

  18. Quantification of the adrenal cortex hormones with radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Badillo A, V.; Carrera D, A. A.; Ibarra M, C. M., E-mail: vbadillocren@hotmail.co [Universidad Autonoma de Zacatecas, Unidad Academica de Estudios Nucleares, Calle Cipres No. 10, Fracc. La Penuela, 98068 Zacatecas (Mexico)

    2010-10-15

    The pathologies of the adrenal cortex -adrenal insufficiency and Cushing syndrome- have their origin on the deficit or hypersecretion of some of the hormones that are secreted by the adrenal cortex, which is divided in three zones anatomically defined: the external zone, also called the zona glomerulosa, which is the main production site of aldosterone and mineralocorticoids; the internal zone, or zona reticularis, that produces androgens; and the external zone, or zone 1 orticotrop, which is responsible for producing glucocorticoids. In this work, a quantitative analysis of those hormones and their pathologic trigger was made; the quantification was made in the laboratory by means of highly sensitive and specific techniques, in this case, the radioimmunoassay, in which a radioisotope I-125 is used. This technique is based on the biochemical bond-type reaction, because it requires of a substance called the linker, which bonds to another called ligand. This reaction is also known as antigen-antibody (Ag-Ab), where the results of the reaction will depend on the quantity of antigen in the sample and on its affinity for the antibody. In this work, a 56 patients (of which 13 were men and 43 women) study was made. The cortisol, the ACTH, the androsterone and the DHEA values were very elevated in the majority of the cases corresponding to women, predominating cortisol; while in men, a notorious elevation of the 17 {alpha}-OH-PRG and of the DHEA-SO{sub 4} was observed. Based on that, we can conclude that 51 of them did not have mayor complications, because they just went to the laboratory once, while the remaining 5 had a medical monitoring, and they visited the laboratory more than one occasion, tell about a difficulty on their improvement. According to the results, an approximate relation of 8:2 women:men, respectively, becomes clear to the hormonal pathologies of the adrenal cortex. (Author)

  19. Development of a Radioimmunoassay for Bovine Chymosin B

    Directory of Open Access Journals (Sweden)

    Borceux, JP.

    2007-01-01

    Full Text Available The present study was conducted to develop and validate a specific radioimmunoassay system for measurement of bovine chymosin B (bChyB concentrations in plasma samples. Bovine ChyB was used for immunization of rabbits and as standard and tracer. Chymosin B concentrations were measured in plasma samples from two groups of calves (Group 1: calves sampled from birth to 24 hours; Group 2: calves sampled from Day 1 to 21 after birth and from one cow during the peri-partum period. Detection limit of the assay was 9.0 ng/ml. Recovery was higher than 89.3%. Repeatability and reproducibility ranged from 1.52% to 5.23% and from 1.52% to 12.57% respectively. No cross-reaction was found with pepsinogen A from bovine, porcine or human origins. In Group 1, bChyB concentrations increased from 47.3 ± 45.1 ng/ml (5 min after birth to 325.5 ± 161.2 ng/ml (12 hours after birth, then no significant change was observed till 24 hours after birth (293.0 ± 161.5 ng/ml. In Group 2, concentrations decreased from Day 1 (455.3 ± 191.1 ng/ml to Day 21 (117.9 ± 85.1 ng/ml. In adult cow, mean concentration was 136.0 ± 32.3 ng/ml. In conclusion, bChyB is able to cross the stomach basal membrane and to reach the blood circulation at detectable levels in both young calves and adult cows.

  20. Evaluation of a radioimmunoassay for the determination of anti-native DNA antibodies. Evaluation d'une technique radio-immunologique pour la detection des anticorps anti-ADN natif

    Energy Technology Data Exchange (ETDEWEB)

    Johanet, C.; Soulie, E.; Absalon, Y.B.; Ocwieja, T.; Abuaf, N. (Hopital Saint-Antoine, 75 - Paris (FR))

    1991-01-01

    The anti-native DNA antibodies were measured by a radioimmunoassay (RIA) type Farr assay in the sera from 648 patients: 108 with active or inactive systemic lupus erythematosus (SLE), 181 with clinical symptoms of another connective tissue disease, 171 with liver diseases, 29 with different pathology and 159 normal sera were obtained from a blood bank. The anti-DNA kit has been calibrated against the first international units/ml. This assay has proved to be sensitive and specific, and appears to be reliable for the diagnosis and follow-up of SLE patients. The authors propose a new reference cut-off level higher than producer's one.

  1. Development, validation, and application of a urinary relaxin radioimmunoassay for the diagnosis and monitoring of pregnancy in felids.

    Science.gov (United States)

    de Haas van Dorsser, Florine J; Swanson, William F; Lasano, Salamia; Steinetz, Bernard G

    2006-06-01

    Many nondomestic felids are highly endangered, and captive breeding programs have become essential components of holistic conservation efforts for these species. The ability to diagnose pregnancy early in gestation is fundamental to developing effective breeding programs. The purpose of this study was to develop a radioimmunoassay (RIA) for the detection of urinary relaxin in felids and assess its applicability for early, noninvasive pregnancy diagnosis in domestic cats (Felis silvestris catus) and leopards (Panthera pardus). Urine was collected from pregnant and nonpregnant domestic cats and leopards at mating, and then weekly thereafter for the duration of gestation. Paired serum samples were also collected from the domestic cats. A RIA for relaxin that uses an antiserum against synthetic canine relaxin was validated for felid urine and shown to detect relaxin immunoreactivity in pregnant cat urine subjected to acid-acetone extraction. In the cat, urinary relaxin was first detected between Days 21 and 28 of gestation; levels peaked at 42-49 days, and the concentrations then declined over 2 wk prior to parturition. The urinary relaxin profiles of the cat mirrored those in serum. In the leopard, urinary relaxin was first detected at Day 25-28 of gestation; levels peaked at Day 60-64 and declined in the last 3-4 wk of pregnancy. These results indicate that measurement of urinary relaxin in the cat and leopard provides a reliable method for pregnancy determination from as early as 3-4 wk of gestation. This method of pregnancy diagnosis and monitoring may prove useful in the breeding management of domestic cats and other felid and canid species, and provides a foundation for future studies on pregnancy in captive exotic carnivores.

  2. Radioimmunoassay in Ascidiella aspersa of a gonadoliberin (GnRH)-like factor with an apparent molecular weight higher than that of mammalian decapeptide

    Energy Technology Data Exchange (ETDEWEB)

    Dufour, S.; Monniot, F.; Monniot, C. and others

    1988-02-21

    A radioimmunoassay (RIA) for mammalian gonadoliberin (mGnRH) showed the presence of a GnRH-like factor in the neural complex of a Protochordate, Ascidiella aspersa (about 0.6 pg eq mGnRH/complex). The slope of the displacement curves was slightly lower than with mGnRH indicating antigene differences. No cross reactive material was found in mantle and siphonal area. The KD on Sephadex G25 was 0.45 versus 0.90 with mGnRH. That suggests that the molecular weight of the Ascidian GnRH-like factor is higher than that of known Vertebrate GnRH's, possibly due to a different processing of the precursor.

  3. Radioimmunoassay of erythropoietin in chronic uraemia or anephric patients

    Energy Technology Data Exchange (ETDEWEB)

    Naets, J.P.; Waks, D.; Garcia, J.F.; Tousaaint, C.; Buset, M.

    1986-01-01

    The erythropoietin (Ep) plasma titre of 41 anaemic patients with normal renal function or with renal insufficiency (with or without kidney) was measured by RIA and compared to values observed with the polycythaemic mouse assay. A good correlation was found with both methods (r = 0.88). However, about 20% of samples gave higher values with the bioassay. 21 Ep titres measurable by the bioassay ranged from 40 to 4400 mU (mean 717.1 mU) compared to 14.8 to 3788 mU (mean 484.7 mU) measured by the RIA. Low levels of Ep plasma titre have been observed in patients with renal insufficiency and no difference was found between nephric uraemic patients and the anephric group. These results suggest that the increased blood requirements in anephrics are not due to a smaller production of Ep, but ultimately to the presence of an inhibitor of erythropoiesis. The well-known inverse relationship between haematocrit and Ep level was not found in renal insufficiency. However, some humoral regulation of erythropoiesis seems to persist in these patients since the elevation of red blood cells by transfusions was followed by a decrease of the Ep level.

  4. Radioimmunoassay for herpes simplex virus (HSV) thymidine kinase

    Energy Technology Data Exchange (ETDEWEB)

    McGuirt, P.V.; Keller, P.M.; Elion, G.B.

    1982-01-30

    A sensitive RIA for HSV-1 thymidine kinase (TK) has been developed. This assay is based on competition for the binding site of a rabbit antibody against purified HSV-1 TK, between a purified /sup 3/H-labeled HSV-1 TK and a sample containing an unknown amount of viral TK. The assay is capable of detecting 8 ng or more of the HSV enzyme. Purified HSV-1 TK denatured to <1% of its original kinase activity is as effective in binding to the antibody as is native HSV-1 TK. Viral TK is detectable at ranges of 150-460 ng/mg protein of cell extract from infected cells or cells transformed by HSV or HSV genetic material. HSV-2 TK appears highly cross-reactive, VZV TK is slightly less so, and the vaccinia TK shows little or no cross-reactivity. This RIA may serve as a tool for monitoring the expression of the HSV TK during an active herpes virus infection, a latent ganglionic infection, or in neoplastic cells which may have arisen by viral transformation.

  5. Radioimmunoassay and characterization of enkephalins in rat tissues

    Energy Technology Data Exchange (ETDEWEB)

    Miller, R.J.; Chang, K.J.; Cooper, B.; Cuatrecasas, P.

    1978-01-25

    A highly sensitive and specific radioimmunoassay for three enkephalins (opiate-like pentapeptides) has been developed. The assay utilizes /sup 125/I-labeled enkephalins and antisera raised in rabbits, to synthetic enkephalins coupled with glutaraldehyde to bovine serum albumin. These antisera show <1% cross-reactivity between H/sub 2/N-Tyr-Gly-Gly-Phe-Met-OH ((Met/sup 5/)enkephalin) and H/sub 2/N-Tyr-Gly-Gly-Phe-Leu-OH ((Leu/sup 5/)enkephalin) and even lower cross-reactvity to ..beta..-endorphin. Morphine shows no cross reactivity at all. The assay can detect as little as 10 fmol of enkephalin. Dose response curves for synthetic enkephalin and enkephalin-like immunoreactivity in acid extracts of brain are superimposable. Enkephalin-like immunoreactivity in tissue extracts is destroyed by treating extracts with leucine aminopeptidase or carboxypeptidase, enzymes which also destroy synthetic enkephalin. An opiate receptor binding assay based on the binding of /sup 125/I-labeled (DAla/sup 2/Leu/sup 5/)-enkephalin to N4TG1 neuroblastoma cells has also been developed. Gel filtration of acid extracts of brain or pituitary gland shows that both contain opiate-like material. However, whereas in brain most of opiate-like material co-chromatographs with enkephalin, in the pituitary no opiate-like material was observed to co-chromatograph with enkephalin but was of higher molecular weight. Enkephalin is widely distributed in the brain. High concentrations of both (Met/sup 5/)- and (Leu/sup 5/)enkephalins are found in the striatum (approximately 5 pmol/mg of protein), lower concentrations in the thalamus and midbrain, and very low concentrations in the cerebellum. The ratio of (Met/sup 5/)enkephalin to (Leu/sup 5/)enkephalin also differs in different brain areas. The ratio is very high in the hippocampus (15.2) and hypothalamus (13.6), but lower in other areas such as the cortex (1.4).

  6. New sensitive direct radioimmunoassay for human plasma renin and its clinical application

    Energy Technology Data Exchange (ETDEWEB)

    Higaki, J.; Ogihara, T.; Imai, N.; Kumahara, Y.; Hontani, S.; Nishiura, M.; Ogawa, H.; Hirose, S.; Murakami, K.

    1984-12-01

    A new sensitive direct radioimmunoassay for human plasma renin has been developed. Renin was purified from Haas' preparation utilizing a pepstatin-C/sub 6/-Sepharose affinity chromatography. Antiserum, prepared by immunizing rabbits with the purified renin, was used for the direct radioimmunoassay at a final dilution of 1:30,000. The antibody was specific for human renal and plasma renin, but did not cross-react with cathepsin D, trypsin, or renins of mouse, dog, and rat. Radioimmunoassay was performed by the double antibody technique using the delayed tracer addition method. In this method, a standard curve was obtained over a range from 0.2 to 8.0 ng/ml. The values from this assay correlated well with total renin activity measured as the generation rate of angiotensin I after trypsin activation, but correlated weakly with active renin activity. This finding disclosed that both active and inactive renin were detected by this method. In normal participants, plasma renin concentration determined by direct radioimmunoassay was increased by standing and furosemide injection. The plasma renin concentration determined by direct radioimmunoassay of patients with essential hypertension was not significantly different from values in normal controls. The values were higher in patients with renovascular hypertension, malignant hypertension and Bartter's syndrome, but lower in patients with primary aldosteronism than in normal controls. 20 references, 7 figures.

  7. Quantitative determination of albumin in urine (microalbuminuria) by electroimmunoassay and radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Zanazzi, M.; Sverzellati, E.; Scarpioni, L.; Pantano, E.; Cerri, L.; Cecchettin, M.

    1986-10-01

    Electroimmunoassay and radioimmunoassay are two methods specifically designed for rapid and accurate analysis of subclinical amounts of proteins in urine. These assays are very sensitive to detect low concentrations of albumin in urines (17-75 mg/24 hrs) and to discover patients with microalbuminuria. Electroimmunoassay and radioimmunoassay are similar for precision and accuracy. Electroimmunoassay results can be obtained within few hours (3 hrs) and a remarkable advantage is the low cost of the equipment and the reagents. Radioimmunoassay results can be obtained within 2 hours. This assay is useful to perform multiple assays in big laboratories, but electroimmunoassay is much more convenient for little assays, when there are few urine samples to investigate.

  8. Standardization of radioimmunoassay for dosage of angiotensin II (ang-II) and its methodological evaluation; Padronizacao do radioimunoensaio para dosagem de angiotensina II (ang-II) e sua validacao metodologica

    Energy Technology Data Exchange (ETDEWEB)

    Mantovani, Milene; Mecawi, Andre S.; Elias, Lucila L.K.; Antunes-Rodrigues, Jose, E-mail: llelias@fmrp.usp.b, E-mail: antunes@fmrp.usp.b [Universidade de Sao Paulo (FMRP/USP), Ribeirao Preto, SP (Brazil). Faculdade de Medicina

    2011-10-26

    This paper standardizes the radioimmunoassay (RIA) for dosage of ANG-II of rats, after experimental conditions of saline hypertonic (2%), treating with losartan (antagonist of ANG-II), hydric privation, and acute hemorrhage (25%). After that, the plasmatic ANG-II was extracted for dosage of RIA, whose sensitiveness was of 1.95 pg/m L, with detection of 1.95 to 1000 pg/m L. The treatment with saline reduced the concentration of ANG-II, while the administration pf losartan, the hydric administration and the hemorrhage increase the values, related to the control group. Those results indicate variations in the plasmatic concentration of ANG-II according to the experimental protocols, validating the method for evaluation of activity renin-angiotensin

  9. Heterophilic antibodies interfering with radioimmunoassay. A false-positive pregnancy test

    Energy Technology Data Exchange (ETDEWEB)

    Vladutiu, A.O.; Sulewski, J.M.; Pudlak, K.A.; Stull, C.G.

    1982-11-19

    A young woman with amenorrhea had a consistently positive pregnancy test result (serum radioimmunoassay measurement of ..beta..-human chorionic gonadotropin hormone). No fetal or placental tissue was found after uterine curettage and exploratory laparotomy. The false-positive pregnancy test result was due to heterophilic antibovine and antigoat antibodies in the patient's serum. These antibodies interfered with radioimmunoassays using goat antibodies. This case shows that serum heterophilic antibodies can interfere with immunoassays and result in unnecessary diagnostic procedures and/or unnecessary treatment.

  10. Bone marrow acid phosphatase by radioimmunoassay. [/sup 125/I; prostatic carcinomas

    Energy Technology Data Exchange (ETDEWEB)

    Belville, W.D.; Cox, H.D.; Mahan, D.E.; Olmert, J.P.; Mittemeyer, B.T.; Bruce, A.W.

    1978-06-01

    A double-antibody radioimmunoassay was developed and utilized to measure prostatic acid phosphatase in bone marrow aspirates. One hundred-eighteen patients with carcinoma of the prostate in various clinical stages, and fifty with benign prostatic hyperplasia were studied. In patients with carcinoma, levels of prostatic acid phosphatase in bone marrow aspirates were found to correlate well with increasing clinical stage of the disease. Determination of bone marrow prostatic acid phosphatase by radioimmunoassay may be a valuable adjunct to clinicopathologic staging of prostatic carcinoma.

  11. IN VITRO CELL CULTURE AND HORMONE RADIOIMMUNOASSAY OF HUAMAN PITUITARY ADENOMAS

    Institute of Scientific and Technical Information of China (English)

    陆汉魁; 林祥通; 等

    1994-01-01

    Tissues from 30 human pituitary adenomas are monolayer-cell-cultured in vitro.Hormone secretion of GH,PRL,TSH,LH and FSH by cells into medium is detected by radioimmunoassay .The pattern and amount of hormone(s0 in the medium are used to determine the nature of the cells and thus to establish functional classification of pituitary adenomas.The results show that cell culture technique provides and easy and suitable mode for investigating the nature of pituitary adenomas.Hormone radioimmunoassay of culture medium is precise and reliable and represents the whole adenoma tissue.Further studies can lead to clearer understandngs of the pathology of pituitary adenomas.

  12. Examinations of hens' eggs on residues of Chloramphenicol using a radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Scherk, F.; Agthe, O.

    In the Federal Republic of Germany the application of Chloramphenicol to animals used for human food supply is restricted by law. Milk and dairy products as well as hen's eggs and egg products are not allowed to contain more than 1 ppb Chloramphenicol. 18 hens were fed with water treated with Chloramphenicol. The eggs of the treated animals were then analysed by means of radioimmunoassay. The applied radioimmunoassay is suitable for routine analysis to a minimum detection limit of 1 ppb. 378 eggs from the Weser-Ems district were tested for Chloramphenicol. No sample contained Chloramphenicol.

  13. Strategies for detection of transfusion-transmitted viruses eluding identification by conventional serologic tests. I. Radioimmunoassay for picogram quantities of DNA

    Energy Technology Data Exchange (ETDEWEB)

    Neurath, A.R.; Strick, N. (New York Blood Center, NY (USA))

    1983-09-01

    The unavailability of serological tests for detection of several not yet characterized infectious agents transmitted by blood transfusion or by blood products prompted the development of alternative tests based on utilization of labeled nucleic acid probes specific for genomes of each of these agents. The prerequisite for the preparation of such probes is the demonstration in human plasma of nucleic acid sequences distinct from those present in host DNA or in genes of already characterized viruses occurring in plasma of infected individuals. To accomplish this, ultrasensitive tests for nucleic acids not dependent on their base sequence are needed. The authors describe a radioimmunoassay (RIA) for picogram quantities of DNA. Plasma (serum) specimens are treated with proteinase K in the presence of sodium dodecyl sulfate and extracted with phenol. Nucleic acids are precipitated with ethanol in the presence of dextran (mol.wt. approx. 5X10/sup 5/) as carrier. Subsequently, DNA from the redissolved samples is adsorbed onto polylysine-coated wells of microtiter plates and detected by a double-antibody RIA using anti-DNA autoantibodies from NZB/NZW mice and /sup 125/I-labelled antibodies to mouse immunoglobulins. DNA which did not hybridize with human DNA was detected by this method in sera containing hepatitis B virus used as a model system.

  14. Tissue distribution and subcellular localization of phosphatidylcholine transfer protein in rats as determined by radioimmunoassay

    NARCIS (Netherlands)

    Teerlink, T.; Krift, T.P. van der; Post, M.; Wirtz, K.W.A.

    1982-01-01

    A radioimmunoassay for the phosphatidylcholine-transfer protein from rat liver was used to measure levels of PC-transfer protein in rat tissues. The assay as described before (Teerlink T., Poorthuis B.J.H.M., Van der Krift T.P. and Wirtz K.W.A., Biochim. Biophys. Acta 665 (1981) 74–80) was modified

  15. Radioimmunoassay measurement of plasma oxytocin and vasopressin in cows during machine milking

    Energy Technology Data Exchange (ETDEWEB)

    Landgraf, R.; Wehowsky, G.; Schulz, J.; Schulze, H.; Bothur, D. (Forschungsinstitut fuer Koerperkultur und Sport, Leipzig (German Democratic Republic); Karl-Marx-Universitaet, Leipzig (German Democratic Republic). Sektion Tierproduktion und Veterinaermedizin)

    1982-07-01

    The response of plasma oxytocin and vasopressin to machine milking in cows was studied by radioimmunoassay. Depending on the method of machine milking used, plasma oxytocin increased to a greater or lesser degree after teat cup application. Plasma vasopressin was not affected by the milking procedures.

  16. A bioequivalency study of two trifluoperazine tablet formulations using RIA and GC-MS.

    Science.gov (United States)

    Midha, K K; Hawes, E M; Korchinski, E D; Hubbard, J W; McKay, G; Cooper, J K; Roscoe, R M

    1984-01-01

    Two sensitive analytical procedures, a radioimmunoassay (RIA) and a mass fragmentographic (GC-MS) method, were used to quantitate plasma trifluoperazine concentrations over 24 h in five healthy male volunteers following single 5 mg doses of two trifluoperazine tablet formulations (A and B) in a two-way cross-over design. Bioavailability in terms of area under the plasma concentration versus time curve to 24h or extrapolated to infinity, maximum plasma concentration and time to maximum plasma concentration using either RIA or GC-MS was not statistically significantly different from one formulation to the other. Also, there were no statistically significant differences between GC-MS and RIA values for AUC24(0) and Cmax for each of the two formulations examined. However, the mean AUC24(0) RIA/GC-MS ratios for formulations A and B were 3.1 and 3.4, respectively, while the mean Cmax RIA/GC-MS ratios were 1.7 and 2.1, respectively. These differences in AUC and Cmax are probably mainly due to the relative non-specificity of the RIA antiserum. Thus, where GC-MS is preferred for pharmacokinetic studies, both analytical procedures can be used for comparative single-dose bioequivalence studies of trifluoperazine. However, both the methods should be tested in patients in order to establish the suitability of one procedure over the other for the study of plasma level versus clinical response correlations.

  17. Development of reagents for radioimmunoassay of: triiodothyronine, thyroxine and thyrotrophin; Desarrollo de reactivos para el radioinmunoanalisis de: triyodotironina, tiroxina y tirotrofina

    Energy Technology Data Exchange (ETDEWEB)

    Delgado S, B.; Lavalley E, C.; Ruiz J, A.; Garcia F, C.; Zamorano A, F

    1991-12-15

    The radioimmunoassay (RIA) of thyroid hormones it is the but it frequents of all the studies carried out by RIA in the laboratories of Nuclear Medicine, these essays are carried out with imported reagents. In the ININ the reagents and the necessary methodology have been developed for the triiodothyronine (T3), thyroxine (T4) and thyrotrophin (TSH). The good titles of the antibodies (Ac) primary for each hormone were of 1:4,000; 1:750 and 1:1,500. The used separation system was of double Ac with PEG to 10%, with titles of 1:10 for the second Ac of lamb. The specific activity for 125-I-T3 and 125-I-T4 oscillate between 850 at 900 {mu}Ci / {mu} g: being this of 90 {mu} Ci /{mu}g for TSH. To the first two hormones they were added 1-8 aniline naftalen sulfonic acid (ANS) to concentrations of 3 and 2 mg/ml respectively. As buffer for T3 and T4 it was used Tris-HCl pH 8.6 and PBS with normal serum of rabbit (SNC) for TSH. The standards got ready in buffer or free serum of thyroid hormones. The slope of the standard curves varied between -2.3 to -2.7 and the variation intra and inter assay among 4 to 10%. It is had at the moment in the ININ with standardized reagents for the RIA of T3, T4 and TSH, it is hoped to carry out tests in other laboratories and to establish the conditions of stability more appropriate to begin the preparation of pilot reagents. (Author)

  18. Setting-up and validation of two radioimmunoassay methods for determination of plasma progesterone concentration in mares, cows and rats; Padronizacao e validacao de dois metodos de radioimunoensaio (RIE) para dosagem da progesterona (P{sub 4}) no plasma de equinos, bovinos e ratos

    Energy Technology Data Exchange (ETDEWEB)

    Rosa e Silva, A.A.M.; Caldas, M.C.S.; Campos, L.M.A. [Sao Paulo Univ., Ribeirao Preto, SP (Brazil). Fac. de Medicina. Dept. de Fisiologia; Gradela, A. [UNESP, Jaboticabal, SP (Brazil). Faculdade de Ciencias Agrarias e Veterinarias

    1993-06-01

    Two reliable radioimmunoassay (RIA) methods which permits the measurement of progesterone (P{sub 4}) in plasma of equine, bovine and rats are described. After extraction of plasma with diethylic ether the RIA methods were performed. The first one utilizes {sup 125} I progesterone (double antibody method) and the other 1,2,6,7,16, 17 {sup 3} H progesterone (adsorption in charcoal/dextran). Both two methods were suitable in the valuation of plasma P{sub 4} concentration in different physiological reproductive conditions. The method of the double antibody showed higher sensibility beyond to be less expensive than the other method. Despite it, the two RIA methods were much less expensive than available commercial Kits in the market. (author) 14 refs., 2 figs., 1 tab.

  19. A solid phase radioimmunoassay for free triiodothyronine in serum:assay development and validation

    Institute of Scientific and Technical Information of China (English)

    1999-01-01

    A solid phase radioimmunoassay for free triiodothyroninein serum was developed based on double-antibody coated tubes.The method was turned out to be reliable with good reproducibility,higher sensitivity and easy performance.The measurable range of FT3 in serum was 1.2 to 38pmol/L.The mean coefficients of variationwithin and between assays were 1.79%~3.18% and 4.72%~9.31%,respectively.The FT3 concentrations in euthyroid serum as determined by this methodwere 2.8 to 7.8pmol/L.The FT3 values determined by this new methodcorrelated well with those measured by a commercial radioimmunoassay(r=0.853).

  20. Radioimmunoassay of renin-angiotensin-aldosterone in patients with adrenal tumors

    Energy Technology Data Exchange (ETDEWEB)

    Slavnov, V.N.; Yakovlev, A.A.; Yugrinov, O.G.; Gandzha, T.I. (Kievskij Nauchno-Issledovatel' skij Inst. Ehndokrinologii i Obmena Veshchestv (Ukrainian SSR))

    1983-02-01

    The results are presented of a study of the renin-angiotensin-aldosterone system in 89 patients with aldosteronoma, corticosteroma, pheochromocytoma and hypertension. Radioimmunoassay was used to measure aldosterone concentration and renin activity in the peripheral blood and blood from vena cava inferior, the renal and adrenal veins, the circadian cycle of their content and the responsiveness of the glomerular zone of the adrenal cortex and the juxtaglomerular renal system under the influence of lasix intake and the change over from a horizontal into vertical position. Patients with adrenal tumors have shown disorders of renin-angiotensin-aldosterone function. Radioimmunoassay of the renin-angiotensin-aldosterone system promotes early detection of adrenal tumors in the general population of patients with hypertension and can be used for control over therapeutic efficacy.

  1. Quantification of the N-terminal propeptide of human procollagen type I (PINP): comparison of ELISA and RIA with respect to different molecular forms

    DEFF Research Database (Denmark)

    Jensen, Charlotte Harken; Hansen, M; Brandt, J

    1998-01-01

    This paper compares the results of procollagen type I N-terminal propeptide (PINP) quantification by radioimmunoassay (RIA) and enzyme linked immunosorbent assay (ELISA). PINP in serum from a patient with uremic hyperparathyroidism was measured in RIA and ELISA to 20 micrograms l-1 and 116...... micrograms l-1 and the corresponding concentrations in dialysis fluid were 94.5 micrograms l-1 and 140 micrograms l-1, respectively. PINP antigen appears in two distinct peaks following size chromatography and the two peak fractions display immunological identity and identical M(r)'s (27 kDa: SDS...

  2. Homologous radioimmunoassay for salmon gonadotropin releasing hormone s-Gn-RH

    Energy Technology Data Exchange (ETDEWEB)

    Breton, B.; Motin, A. (I.N.R.A., Campus de Beaulieu, 35 - Rennes (France)); Kah, O.; Lemenn, F.; Geoffre, S.; Precigoux, G.; Chambolle, P. (Universite de Bordeaux-I, 33 - Talence (France))

    1984-09-30

    A radioimmunoassay for Salmon Gn-RH (p-gly-His-Trp-Ser-Tyr-Gly-Trp-Leu-Pro-Gly) (NH/sub 2/) has been developed with a sensitivity of 7 pg/assay tube. The system allows the specific detection of an immunological GnRH related substance in the brain and pituitary of three teleost species but not in an elasmobranch the Dogfish. These results are discussed and some Gn-RH contents of the organs are proposed.

  3. Interferences in radioimmunoassay of aflatoxins in food and fodder samples of plant origin

    Energy Technology Data Exchange (ETDEWEB)

    Rauch, P.; Fukal, L.; Brezina, P.; Kas, J.

    Cross-reactions and resulting nonspecific binding of substances with structures resembling aflatoxins (derivatives of coumarin, and cinnamonic and benzoic acids, etc.) were investigated. The concentrations of these substances causing erroneously high or false positive values in radioimmunoassay were determined. One ..mu..g aflatoxin B/sub 1//kg sample may be simulated by the occurrence of 5 g coumarin, 10 g caffeic acid, 16 g chlorogenic acid, or 15 g vanillin/kg fodder or food sample.

  4. [Homologous radioimmunoassay of a hypothalamic factor stimulating salmon pituitary gonodatropic function (sGnRH)].

    Science.gov (United States)

    Breton, B; Motin, A; Kah, O; Lemenn, F; Geoffre, S; Precigoux, G; Chambolle, P

    1984-01-01

    A radioimmunoassay for Salmon Gn-RH p-gly-His-Trp-Ser-Tyr-Gly-Trp- Leu-Pro-Gly) (NH2) has been developed with a sensitivity of 7 pg/assay tube. The system allows the specific detection of an immunological GnRH related substance in the brain and pituitary of three teleost species but not in an elasmobranch the Dogfish. these results are discussed and some Gn-RH contents of the organs are proposed.

  5. Competitive binding radioassays for 1 -25(OH)2 vitamin D; comparative evaluation of two receptor assays and a radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Jallet, P.; Bidet, M.; Audran, M.

    1985-01-01

    The performances of a 1 ,25-dihydroxy vitamin D assay using the cytosol receptor of bovine thymus gland were evaluated and compared to the results obtained with an assay based on cytosol receptor of chick intestine and with a radioimmunoassay.

  6. Memória

    OpenAIRE

    Carlos Alberto Mourão Júnior; Nicole Costa Faria

    2015-01-01

    ResumoEste artigo tem como objetivo central apresentar os processos de memória de maneira didática, proporcionando aos alunos e futuros pesquisadores um primeiro contato satisfatório com o tema. Já há algum tempo, tem sido observada a ocorrência de confusões conceituais e metodológicas no campo da neurociência cognitiva, tanto em relação à memória quanto em relação às outras funções psicológicas básicas. Neste ensaio, alguns conceitos principais são esclarecidos. É apresentada uma classificaç...

  7. Comparison of an enzyme-immunoassay with a radio-immunoassay ...

    African Journals Online (AJOL)

    1990-07-21

    Jul 21, 1990 ... the HBsAg marker but that the RIA test was more sensit!ve for anti-HBs and .... The EIA method used in the study has several advantages over the RIA ... but the most important criter ia when comparing serological methods ...

  8. Sensitive and selective radioimmunoassay for serum 24, 25-dihydroxycholecalciferol in man

    Energy Technology Data Exchange (ETDEWEB)

    Hummer, L.; Christiansen, C. (Glostrup Sygehus, Copenhagen (Denmark))

    1984-07-01

    A sensitive and selective radioimmunoassay for 24,25-dihyroxycholecalciferol (24,25(OH)/sub 2/D/sub 3/) has been developed. Antisera with a high titre and affinity to 24,25(OH)/sub 2/D/sub 3/ were raised in rabbits immunized with a protein conjugate of the 3-hemisuccinate derivative of 24,25(OH)/sub 2/D/sub 3/. Serum samples were extracted by methanol/dichloromethane and the lipid extracts purified on Sephadex LH 20 and chromatographed by single step HPLC on a straight phase silica column. The radioimmunoassay is capable of measuring 24,25(OH)/sub 2/D/sub 3/ in the '24,25(OH)/sub 2/D complex' isolated by HPLC and containing the comigrating metabolites: 24,25(OH)/sub 2/D/sub 3/, 24,25(OH)/sub 2/D/sub 2/, 25(OH)D/sub 3/-26,23 lactone and 25,26(OH)/sub 2/D/sub 2/. The detection limit of 2 pg/assay tube is marked improvement compared to the competitive protein binding assay using rachitic rat serum. Measured by the radioimmunoassay 24,25(OH)/sub 2/D/sub 3/ ranged from 0.05 to 1.96 ng/ml with a mean of 0.85 ng/ml in 34 healthy adults. For comparison 24,25(OH)/sub 2/D, measured simultaneously by competitive protein binding with rachitic rat serum, ranged from 0.1 to 4.0 ng/ml with a mean of 1.76 ng/ml.

  9. Radioimmunoassay for zearalenone and zearalanol in human serum: production, properties, and use of porcine antibodies

    Energy Technology Data Exchange (ETDEWEB)

    Thouvenot, D.; Morfin, R.F.

    1983-01-01

    To produce antigens susceptible to raise antibodies for resorcylic acid lactones, the 6'-carboxymethyloxime derivatives of zearalenone and zearalanone were bound to bovine serum albumin. Pigs could be immunized by using these antigens, the best titer in antibodies being obtained with the zearalenone antigen. The procine antibodies were specific for the resorcylic acid lactones of structural resemblance with zearalenone. This specificity made the antibodies usable for a radioimmunoassay of zearalenone and zearalanol, which may be found in human and animal sera. The range of the assay was between 0.25 and 10 ng. The limit of detection was 5 ppb (5 ng/ml) in human serum.

  10. Use of radioimmunoassay procedures for the determination of sex hormones in animal tissues

    Energy Technology Data Exchange (ETDEWEB)

    Hoffmann, B. (Institut fuer Veterinaermedizin des Bundesgesundheitsamtes (Robert von Ostertag-Institut), Berlin (Germany, F.R.))

    1983-07-01

    Radioimmunoassay methods for the determination of sex steroids and other compounds with sex hormone-like activities in various edible animal tissues and endocrine glands have been developed. Reliability of these methods, allowing quantification in a range of 10/sup -11/ M, has been adequately demonstrated. When applied to monitoring residues of anabolic sex hormones in edible tissues of veal calves, physiological baseline levels of some endogenous ''anabolic'' steroids (like testosterone, oestrogens) were established; in the case of xenobiotics residues at the scheduled time of slaughter could be quantified (trenbolone) and a regulatory method to implement the ban of diethylstilbestrol was introduced.

  11. Urine aldosterone radioimmunoassay: validation of a method without chromatography. [/sup 3/H

    Energy Technology Data Exchange (ETDEWEB)

    Brown, R.D.; Swander, A.; McKenzie, J.K.

    1976-05-01

    A simplified radioimmunoassay of urinary aldosterone is reported. Acid-hydrolyzed urine was extracted with dichloromethane and the extract assayed without further purification. Urinary aldosterone values in patients with Cushing's syndrome, low and normal-renin essential hypertension, congenital adrenal hyperplasia, and primary aldosteronism determined by this method agreed closely (r = 0.95, P less than 0.01) with values obtained using a standardized chromatographic method. This simplified assay represents a significant advance in our capabilities for evaluating patients for abnormalities in aldosterone physiology.

  12. Results of the HPL determination using a new enzymatic immunoassay - comparison with a commercial radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Leis, D.; Schneider, B.; Keller, A.; Braun, S.

    1982-01-06

    An enzymatic immunoassay recently developed by the firm Organon, Oss, Netherlands, to determine HPL in the serum of pregnant women was compared to a widely used radioimmunoassay marketed by the firm Amersham-Buchler, Braunschweig. The values determined showed a good correlation. The accuracy of measurement was within the generally accepted range for immunological assays of this kind. The assay procedures are comparable with regard to the expenditure of time and work. A great advantage of the EIA is due to the absence of radioactive substances, which permits this test to be performed in any laboratory equipped with a centrifuge and photometer.

  13. Detection of prostatic cancer by solid-phase radioimmunoassay of serum prostatic acid phosphatase. [/sup 125/I tracer technique

    Energy Technology Data Exchange (ETDEWEB)

    Foti, A.; Cooper, J.F.; Herschman, H.; Malvaez, R.R.

    1977-12-22

    We compared our radioimmunoassay with the standard enzyme assay for prostatic acid phosphatase in the diagnosis of prostatic cancer. Serum samples from 50 controls, 113 patients with prostatic cancer, 36 with benign prostatic hyperplasia, 83 with other cancers, 20 with gastrointestinal disorders and 28 with total prostatectomies were randomized and studied by radioimmunoassay and enzyme assay. When the upper limit was set at 8.0 ng per milliliter (mean + 4 S.D.) the radioimmunoassay diagnosed prostatic cancer in 33, 79, 71 and 92 percent of the patients with Stage I, II, III and IV disease. In contrast, the enzyme assay detected elevations of enzyme in the serum of 12, 15, 29, and 60 percent respectively. No false-positive results were detected by either assay in normal controls but the radioimmunoassay test was positive in two patients with benign prostatic hyperplasia, in one patient after total prostatectomy, in nine with other cancers and in one of the group with gastrointestinal disorders. In contrast to the enzyme assay, the radioimmunoassay distinguished over half the cases of intracapsular prostatic cancer.

  14. Radioimmunoassay for somatomedin C: comparison with radioreceptor assay in patients with growth-hormone disorders, hypothyroidism, and renal failure

    Energy Technology Data Exchange (ETDEWEB)

    Baxter, R.C.; Brown, A.S.; Turtle, J.R.

    1982-03-01

    An antiserum (Tr4) was raised in rabbits against a basic somatomedin C-like peptide preparation. Using high-immunoreactivity somatomedin C tracer, we compared the performance of radioimmunoassays in which we used the Tr4 antiserum distributed by the National Pituitary Agency (NPA) with that of the human placental-membrane somatomedin radioreceptor asay (RRA). In their cross reactivity towards various somatomedin-like and unrelated peptides, the two radioimmunoassay methods were almost identical, although NPA antiserum, with about fourfold higher titer than Tr4 antiserum, showed a slightly greater sensitivity for most peptides tested. Radioimmunoassay of acid-ethanol-extracted plasma samples from normal persons and acromegalic, hypopituitary, hypothyroid, and renal-failure patients revealed no analytical differences between the antisera (for 122 samples, r = 0.979 between methods). Somatomedin values for acromegalic and hypopituitary samples showed no overlap with normals. Values for hypothyroid and pre-dialysis renal-failure samples were significantly lower than normal. By comparison, the RRA showed greater cross reactivity towards some somatomedin-like peptides and gave significantly lower values than radioimmunoassay for acromegalic and hypothyroid plasma extracts, and significantly higher values for hypopituitary and renal-failure samples. We conclude that the radioimmunoassay methods clearly are of greater diagnostic value than RRA for clinical somatomedin measurement.

  15. Development of a radioimmunoassay for the determination of buprenorphine in biological samples

    Energy Technology Data Exchange (ETDEWEB)

    Debrabandere, L.; Boven, M. Van; Daenens, P. (Louvain Univ. (Belgium))

    1993-02-01

    The development of a specific and sensitive radioimmunoassay for the detection of buprenorphine in urine samples is described. With minor adjustments, the assay was also applied to the analysis for buprenorphine in plasma samples. The 2-diazobenzoic acid derivative of buprenorphine has been prepared as a hapten. The immunization of rabbits with the hapten-bovine serum albumin conjugate resulted in the production of antibodies, which cross-reacted with N-dealkylbuprenophine up to about the 90% level. The antibodies showed very low cross-reactivities with the 3-O-glucuronides and with the structural analogue etorphine. The assay was mainly used to prescreen for buprenorphine in urine samples of persons suspected of Temgesic misuse and to determine buprenorphine in plasma samples. A linear calibration graph for buprenorphine was obtained after logit-log regression. The spiking recovery study showed a linear regression. Intra-and inter-assay relative standard deviations were < 4.35 and < 6.36%, respectively. A comparison study of the high-performance liquid chromatographic determination (X) to the radioimmunoassay (Y) resulted in the following regression equation for the urine samples: Y = 1.44 + 1.64 X (n = 32; r 0.910) and Y = 0.007 + 1.58 X (n = 10; r = 0.930) for plasma specimens. The minimum detectable dose of the immunoassay was calculated to be 10 pg ml[sup -1] (Student's t-distribution, p 0.01, degrees of freedom = 8). (Author).

  16. Use of a programmable desk-top calculator for the statistical quality control of radioimmunoassays.

    Science.gov (United States)

    Cernosek, S F; Gutierrez-Cernosek, R M

    1978-07-01

    We have developed an interactive statistical quality-control system for the small- to medium-sized radioimmunoassay laboratory, which can be used in a programmable desk-top calculator instead of the medium- or large-scale computer systems usually required. The design of this quality-control system is modeled after the suggestions of Rodbard and has three components. The first component evaluates the relationship between the measured response variable of the radioimmunoassay and the precision (or variance) of these measurements. This derived relationship is then used in the second component of the system as the basis for the weighting function used to calculate an interative, weighted, least squares regression of the logit-log transformation of the dose-response curve. The third component uses the quality-control parameters statistically calculated from the linearized dose-response curve to monitor whether the assay is "in-control". The calculator tabulates the means and confidence limits for the various parameters and can plot the statistical quality-control charts. The major benefit of this statistical quality-control system is that it allows the real-time computation and plotting of quality-control data with a programmable desk-top calculator.

  17. Detection of pregnancy by radioimmunoassay of a pregnancy serum protein (PSP60) in cattle

    Energy Technology Data Exchange (ETDEWEB)

    Mialon, M.M.; Renand, G.; Camous, S.; Martal, J.; Menissier, F. (Institut National de Recherches Agronomiques (INRA), 78 - Jouy-en-Josas (France))

    1994-01-01

    The accuracy and efficiency of pregnancy diagnoses in cattle by pregnancy serum protein (PSP60) radioimmunoassay, a progesterone radioimmunoassay or oestrus detection were compared. Blood samples were taken from 349 suckling heifers and cows ( 1 191 inseminations) at 28, 35, 50 and 90 d post-insemination for PSP60 determination and at 22-23 d for progesterone. Females were declared nonpregnant when plasma PSP60 concentration was lower than 0.2 ng/ml at 28, 35 and 50 d and 0.5 ng/ml at 90 d. When compared with rectal palpation at 90 d, the accuracy of positive (negative) diagnoses by progesterone assay was 80% (100%) in heifers and 75% (99%) in cows. The accuracy of positive diagnoses by PSP60 assay increased with gestation stage from 90% on d 28 in heifers (74% in cows) to 100% (99% in cows) at the time of rectal palpation. This accuracy was 84% on d 28 in cows when the interval from calving to blood sampling was higher than 115 d. Whatever the stage, the accuracy of negative diagnoses was higher than 90%. Efficiency in detecting pregnant or nonpregnant females on d 28 was equivalent to the progesterone assay. The method for detecting oestrus applied in this experiment was as efficient as the PSP60 or progesterone test at any stage of gestation. The PSP60 test is very flexible, which makes its use particularly interesting in naturally mated suckling herds because of the uncertainty regarding the date of fertilization. (authors).

  18. Radioimmunoassay for anaphylatoxins: a sensitive method for determining complement activation products in biological fluids

    Energy Technology Data Exchange (ETDEWEB)

    Wagner, J.L.; Hugli, T.E.

    1984-01-01

    Activation of the blood complement system generates bioactive fragments called anaphylatoxins. The three anaphylatoxins C3a, C4a, and C5a are released during classical pathway activation while only C3a and C5a are released when the alternative pathway of complement is activated. Radioimmunoassays were designed to individually detect and quantitate the activation fragments C3a, C4a, and C5a in biological fluids without interference from the precursor molecules C3, C4, and C5. Kinetics of complement activation in fresh human serum exposed to the activators zymosan, heat-aggregated immunoglobulin, or cobra venom factor were monitored using the radioimmunoassay technique. For the first time, activation of components C3, C4, and C5 was followed simultaneously in a single serum sample. Analysis of the patterns and extent of anaphylatoxin formation during activation in serum may be used to screen for deficiencies or defects in the complement cascade. Levels of the anaphylatoxins in freshly drawn serum were much higher than levels detected in EDTA-plasma. Detection of low-level complement activation in patient's blood, urine, or synovial fluid, using anaphylatoxin formation as an indicator, may prove useful in signaling numerous forms of inflammatory reactions. The demonstration of anaphylatoxins in clinical samples is being recognized as a valuable diagnostic tool in monitoring the onset of immune disease.

  19. Tritium labeled amino acid conjugates of prostaglandins and thromboxanes as labeled ligands in prostanoid radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Sautebin, L.; Kindahl, H.; Kumlin, M.; Granstroem, E.

    1985-09-01

    Conjugates of prostaglandins and thromboxanes with tritium labeled amino acids were prepared and employed as labeled ligands in prostaglandin and thromboxane radioimmunoassays. Assays for PGF2 alpha, 15-keto-13, 14-dihydro-PGF2 alpha, TXB2 and 15-keto-13,14-dihydro-TXB2 were evaluated in comparative studies using either these heterologous ligands or the corresponding homologous tritiated eicosanoid as tracers. Binding properties for the respective antibodies were found to be similar using either tracer. Three biological studies were also conducted, viz. study of the release of TXB2 during collagen induced platelet aggregation, of 15-keto-13,14-dihydro-TXB2 during guinea pig pulmonary anaphylaxis, and of PGF2 alpha during bovine luteolysis. The analyses gave comparable results using either the heterologous or the homologous assay. Thus, this type of labeled prostanoid conjugates may serve as a convenient alternative to homologous tracers in radioimmunoassay. Heterologous tracers may even in certain cases provide the only simple solution to the problem of preparing a labeled ligand of high specific activity.

  20. Purification of tracer for somatomedin C radioimmunoassay by hydrophobic interaction chromatography

    Energy Technology Data Exchange (ETDEWEB)

    Baxter, R.C.; Brown, A.S.

    1982-03-01

    A tracer for use in the somatomedin C radiommunoassay by hydrophobic interaction chromatography was purified. Material showing greatest immunoreactivity binds to Octyl Sepharose CL-4B (Pharmacia) in a buffer mixture consisting of 130 mL of acetonitrile and 870 mL of 0.1 mol/L NH/sub 4/HCO/sub 3/, pH 7.8, but is eluted by increasing the acetonitrile content to 180 mL/L. As compared with tracer purified by binding to specific antiserum in liquid phase, precipitating the complex with second antibody, and then dissociating by gel chromatography at acid pH, this tracer shows equal immunoreactivity against specific somatomedin C antiserum. Either preparation allows excellent discrimination between extracts of normal, acromegalic, and hypopituitary plasma samples; thus either is suitable for use in the somatomedin C radioimmunoassay. Tracer purification by hydrophobic interaction chromatography is rapid and inexpensive. It may be useful in preparing highly immunoreactive tracers for other peptide radioimmunoassays.

  1. Interspecies radioimmunoassay for the major internal protein of mammary tumor viruses

    Energy Technology Data Exchange (ETDEWEB)

    Hand, P.H.; Teramoto, Y.A.; Callahan, R.; Schlom, J.

    1980-02-01

    An interspecies radioimmunoassay was developed which detects antigenic determinants shared by type-B mammary tumor viruses (MTVs). This interspecies assay is specific for antigenic sites which the 28,000-dalton major internal protein of MMTVs of laboratory mice (Mus musculus) has in common with polypeptides of MC-MTV. MC-MTV is a new type-B retrovirus isolated from the Asian rodent. Mus cervicolor. Other retrovirus isolates of Mus cervicolor, i.e., M432, CERV-CI, and CERV-CII, as well as other type-C and type-D retroviruses, do not compete in the interspecies assay. The interspecies assay detected MTV cross-reactive antigenic determinants with equal efficiency in milks, lactating mammary glands, and in spontaneous mammary tumors of three distinct species. Particles morphologically indistinguishable from MMTV and MC-MTV have also been detected in Mus cookii mammary tumor cells. The interspecies MTV p28 radioimmunoassay thus provides a potentially useful tool for the detection of etiologically related viruses or viral translational products in species other than the laboratory mouse.

  2. Radioimmunoassay for pantothenic acid in blood and other tissues. [/sup 3/H and /sup 14/C tracer techniques

    Energy Technology Data Exchange (ETDEWEB)

    Wyse, B.W.; Wittwer, C.; Hansen, R.G.

    1979-01-01

    We described a radioimmunoassay for pantothenic acid in biological tissues. D-Pantothenic acid was conjugated with bovine serum albumin by use of a bromoacetyl derivative of pantothenic acid, and antibody to this antigen was raised by injecting it into the foot pads of rabbits. For the radioimmunoassay, a 100-fold dilution of the resulting antiserum was incubated with radiolabeled panthothentic acid. The antibodies were precipitated and dissolved, and the radioactivity of the solution was measured in a liquid scintillation counter. Between 5 and 125 ng of pantothenic acid can be detected in 75 ..mu..L of tissue extract. Validation included recovery and precision studies, parallelism with tissue extracts, and competitive binding studies. Results of the radioimmunoassay and those of microbiological assay with use of Lactobacillus plantarum correlated well (r = 0.80).

  3. Evaluation of the Coat-A-Count sup 125 I fentanyl RIA: Comparison of sup 12 5I RIA and GC/MS-SIM for quantification of fentanyl in case urine specimens

    Energy Technology Data Exchange (ETDEWEB)

    Watts, V.W.; Caplan, Y.H. (Mesa Police Crime Laboratory, AZ (USA))

    1990-09-01

    The Coat-A-Count solid phase {sup 125}I Fentanyl Radioimmunoassay was evaluated with respect to linearity and precision using equine urine fortified with fentanyl and then compared with a gas chromatographic/mass spectrometric method for quantification of fentanyl in urine. The RIA assay was found to be linear over the urine fentanyl concentration range of 0.25 to 7.5 ng/mL and precise with coefficients of variation (CV) ranging from 9.6 to 19.3%. The RIA calibrators, ranging in fentanyl concentrations from 0.25 to 7.5 ng/mL, and controls, at mean fentanyl concentrations of 0.46 and 1.32 ng/mL, were compared by both the RIA and GC/MS methods. The cross-reactivity with the {sup 125}I RIA test was determined for the fentanyl metabolites, norfentanyl and hydroxyfentanyl, and found to be 5% and 35%, respectively. The illicit fentanyl analogs were found to show significant cross-reactivity, ranging from 20 to 100%. The {sup 125}I RIA was compared to GC/MS quantifications of fentanyl in 35 positive and 20 negative case urine specimens.

  4. Preparation of {sup 125}I-iodotyraminehemisuccinyltaxol ({sup 125}ITHT) for competitive taxol radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Tae Sup; Awh, Ok Doo; Choi, Tae Hyun; Kim, Hyun Suk; Hong, Jun Pyo; Lee, Eun Sook [College of Medicine, Yonsei Univ., Wonju (Korea, Republic of); Choi, Chang Woon; Lim, Sang Moo [Korea Cancer Center Hospital, Seoul (Korea, Republic of)

    2002-04-01

    Taxol(Paclitaxel), an antineoplastic agent, has been used in the treatment of ovarian and breast cancers. The determination of optimal Taxol concentrations in human serum was required for enhancing therapeutic effect and maintaining the appropriate Taxol level in blood. This study was aimed to synthesize radiolabeled Taxol derivatives as radiotracer in competitive radioimmuoassay for monitoring Taxol concentrations in blood and to determine the usefulness of its derivatives. Hemisuccinyltaxol(HT) was synthesized by esterification of Taxol with succinic anhydride. Tyraminehemisuccinyltaxol (THT) was synthesized by coupling of HT with tyramine using isobutylchloroformate as coupling agent and purified by HPLC. By using chloramine-T(5.25 mg/ml, 10 {mu}l) as oxidant agent, THT (4 mg/ml, 30 {mu}l) was labeled with {sup 125}I (37 MBq, 1 mCi). To estimate the stability of purified THT, {sup 125}I-iodotyraminehemisuccinyltaxol ({sup 125}ITHT) was dissolved in 80% acetonitrile aqueous solution, and the solution was incubated at 4 .deg. C and 37 .deg. C for 7 days. At various time intervals, the stability of THT and {sup 125}ITHT was monitored. The titer of Taxol monoclonal antibody, 3G5A7, was determined by competitive radioimmunoassay using {sup 125}ITHT as a labeled antigen. A standard dose-response curve was demonstrated by Taxol competitive radioimmunoassay. HT and THT were synthesized with 79.9% and 19.5% yield, respectively. The labeling yield of {sup 125}ITHT was 93%. After 7 days, the chemical purity of THT was 96.5% at 4 .deg. C, and 97.5% at 37 .deg. C. After 3 days, {sup 125}ITHT was stable with 94.7% at 4 .deg. C and 93.4% at 37 .deg. C. After 7 days radiochemical purity was diminished to 88.1% at 4 .deg. C and 86.1% at 37 .deg. C. The titer of Taxol monoclonal antibody, 3G5A7, was determined to 1:256. A standard dose-response cure demonstrated good collinearity (R{sup 2}=0.971) as Taxol concentration-dependent manner. Competitive radioimmunoassay using {sup 125

  5. Estória faz história

    Directory of Open Access Journals (Sweden)

    Vários Autores

    1988-01-01

    Full Text Available Esta seção foi inspirada na estória... "Jogos Internos", do prof. Lino Castellani Filho, que motivou os alunos da disciplina Prática de Ensino e Recreação, para a criação de estórias que falavam da problemática da Educação Física.

  6. Synthesis and radioiodination of analogues of substance P for the building up of a radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Bienert, M.; Schmidt, H.E.; Ehrlich, A.; Forner, K.; Klauschenz, E.; Furkert, J.; Rathsack, R.; Niedrich, H. (Akademie der Wissenschaften der DDR, Berlin. Inst. fuer Wirkstofforschung; Akademie der Wissenschaften der DDR, Berlin-Buch. Zentralinstitut fuer Isotopen- und Strahlenforschung)

    1984-02-01

    The substance P (SP) analogs (Lys(MSOC)/sup 3/)-SP, (Tyr/sup 8/)-SP, (Tyr/sup 8/,Nle/sup 11/)-SP and (p-HPA/sup 1/)-SP have been synthesized by classical methods of peptide synthesis as well as by the liquid phase peptide synthesis (LPPS) to allow conjugation with protein in the N..cap alpha..-position and radioiodination. (Tyr/sup 8/)-SP and (p-HPA-Arg/sup 1/)-SP have been radioiodinated by the chloramine T- and the lactoperoxidase method. A complete S-oxidation of SP was observed, when the chloramine T procedure was used, but this modification does not disturb the assay. The introduction of the (/sup 125/I-pHPA-Arg/sup 1/)-SP-tracer led to considerable improvements of our SP-radioimmunoassay.

  7. Radioimmunoassay of haemoglobin F in K 562 cells following induction with renin substrate and erythropoietin

    Energy Technology Data Exchange (ETDEWEB)

    Rosenloef, K.; Fyhrquist, F.; Hortling, L.; Groenhagen-Riska, C. (Helsinki Univ. (Finland). Minerva Inst. for Medical Research; Helsinki Univ. (Finland). 4th Dept. of Medicine)

    1985-06-01

    To test the hypothesis of renin substrate (RS: angiotensinogen) being a precursor of erythropoietin (EP), the capacity of RS and EP to induce Hb synthesis was compared in cultured human erythroid leukaemia cells of the K 562 line after prestimulation with haemin. For this purpose a radioimmunoassay for haemoglobin F (HbF) was developed. This assay was shown to be specific for HbF, reproducible, and sensitive for 0.1 ng of HbF. The cells were induced by RS and EP to increased HbF production. Cells stimulated with RS or EP showed increased benzidine staining. These data support the hypothesis that renin substrate is a likely precursor of erythropoietin.

  8. Distribution of protein I in mammalian brain as determined by a detergent-based radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Goelz, S.E.; Nestler, E.J.; Chehrazi, B.; Greengard, P.

    1981-04-01

    A radioimmunoassay has been developed for measuring protein I, a basic, neuron-specific protein associated with nerve terminals. The procedure utilizes the detergents NaDodSO/sub 4/ and Nonidet P-40 to prevent nonspecific adsorption of this highly charged protein to various surfaces. By use of this procedure, it has been possible to show that protein I comprises approximately 0.4% of the total protein in cerebral cortex of several mammalian species. In addition, the amount of protein I was determined in about 40 regions of cat brain. The results suggest that measurement of protein I may provide a quantitative method for estimating the density of nerve terminals in various regions of the mammalian nervous system.

  9. Demonstration of circulating 1,24,25-trihydroxyvitamin D/sub 3/ in man by radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Clemens, T.L.; Fraher, L.J.; Sandler, L.M.; O' Riordan, J.L.H. (Middlesex Hospital, London (UK))

    1982-04-01

    1,24,25-trihydroxyvitamin D/sub 3/ has been detected in human serum using a sensitive radioimmunoassay. Tritiated 1,24,25-trihydroxyvitamin D/sub 3/ was synthesized biologically and used as tracer to monitor the recovery of endogenous metabolite during isolation from serum. Circulating 1,24,25(OH)/sub 3/D/sub 3/ in normal subjects ranged from 9.3 to 18.5 pmol/l but was not detectable (< 2.3 pmol/l) in serum from nephrectomized subjects. The trihydroxy metabolite was elevated in three out of four vitamin D deficient subjects who were being treated with vitamin D/sub 3/.

  10. Melatonin in edible plants identified by radioimmunoassay and by high performance liquid chromatography-mass spectrometry

    Energy Technology Data Exchange (ETDEWEB)

    Dubbels, R.; Klenke, E.; Schnakenberg, E.; Ehlers, C.; Schloot, W. [Univ. of Bremen, Center of Human Genetics and Genetic Counselling, Bremen (Germany); Reiter, R.J. [The Univ. of Texas Health Science Center at San Antonio, Dept. of Cellular and Structural Biology, San Antonio, Texas (United States); Goebel, A.; Schiware, H.W. [Gemeinschaftslabor Dr. Schiwara et al., Breman (Germany)

    1995-01-01

    Melatonin, the chief hormone of the pineal gland in vertebrates, is widely distributed in the animal kingdom. Among many functions, melatonin synchronizes circadian and circannual rhythms, stimulates immune function, may increase life span, inhibits growth of cancer cells in vitro and cancer progression and promotion in vivo, and was recently shown to be a potent hydroxyl radical scavenger and antioxidant. Hydroxyl radicals are highly toxic by-products of oxygen metabolism that damage cellular DNA and other macromolecules. Herein we report that melatonin, in varying concentrations, is also found in a variety of plants. Melatonin concentrations, measured in nine different plants by radioimmunoassay, ranged from 0 to 862 pg melatonin/mg protein. The presence of melatonin was verified by gas chromatography/mass spectrometry. Our findings suggest that the consumption of plant materials that contain high levels of melatonin could alter blood melatonin levels of the indole as well as provide protection of macromolecules against oxidative damage. (au) 30 refs.

  11. Study and development of a radioimmunoassay of antidiuretic hormone sensitive at 10/sup -12/M

    Energy Technology Data Exchange (ETDEWEB)

    Caillens, H.; Rousselet, F. (Faculte des Sciences Pharmaceutiques, (France)); Paillard, F. (Hopital Tenon, Paris (France))

    1982-07-01

    A radioimmunoassay of antidiuretic hormone is described. The antiserum was obtained by immunization of rabbits with lysine vasopressin conjugated to hemocyanine. The specificity of the antibody was selective and directed against the pentapeptide ring of the vasopressin molecule: oxytocin showed no cross-reactivity at 10/sup -9/M. The labelled hormone (/sup 125/I-AVP) prepared using the chloramine-T method had a high specific activity (1860 Ci/mmol). Incubation was performed in an equilibrium system. Comparative studies of different separation methods of bounds and free /sup 125/I-AVP showed that the sensitivity and the precision of the standard curve were better using charcoal dextran. The limit of detection of the assay was 1,6 pg per ml.

  12. [ The radioimmunoassay of prostaglandins F in human plasma and endometrium (author's transl)].

    Science.gov (United States)

    Tresguerres, J A; Oriol Bosch, A

    1974-01-01

    A double antibody radioimmunoassay in estudied for the determination of PGF2alpha. Its sensitivity allows to detect 0.4 ng. The reproductibility in this assays is of 7.8 % while between assays is of 10.7 %. No detectable levels (less than 0.8 ng/ml) could be found on blood obtained from the right heart chambers during the menstrual cycle, but they were measured in peripheral blood during normal deliveries. Changes on PGF2alpha concentrations occur in the endometrium along the menstrual cycle. Tis is a relatively simple method useful either for farmocodinamical studies when administering PGF2alpha or for physiological work with tissure showing relatively high concentrations of this compound.

  13. Radioimmunoassay of human homologous prolactin in serum with commercially available reagents. [/sup 125/I tracer technique

    Energy Technology Data Exchange (ETDEWEB)

    Kao, P.C.; Jiang, N.S.; Abboud, C.F.

    1977-09-01

    A clinically useful and reproducible radioimmunoassay for human homologous prolactin, established with commercially available reagents, was studied and validated. We present detailed conditions for iodination and purification of labeled prolactin and the optimal conditions for the assay. By the method, we found values (..mu..g/liter) as follows for serum prolactin: normal men, 8.9 +- 5.2 (mean +- SD); normal women, 11.8 +- 5.5; normal women taking contraceptive pills, 9.2 +- 5.0; pregnant women in the third trimester, 188 +- 69.5; patients with various diseases other than of the hypothalamic-pituitary axis, 9.3 +- 6.3; in some patients with amenorrhea and galactorrhea of diverse origin, 78.2 +- 87.4; and in some patients with surgically proven pituitary tumor, 1414 +- 1980. Results under provocative testing are also presented for a patient with normal hypothalamic-pituitary function.

  14. Radioimmunoassay for progesterone in bovine milk; Radioinmunoensayo para progesterona en leche bovina

    Energy Technology Data Exchange (ETDEWEB)

    Ruiz, Miriam [Instituto Superior de Ciencias y Tecnologia Nucleares, La Habana (Cuba)]. E-mail: mirian@fctn.isctn.edu.cu; Figueredo, Nancy; Castillo, Sonia; Pizarro [Centro de Isotopos, La Habana (Cuba)

    2002-07-01

    A system for the measurement of progesterone in bovine milk by radioimmunoassay has been developed and validated. This assay includes an iodine tracer purified by HPLC, the standard prepared in fat-free milk and an antibody anti-progesterone combined with second antibody. The detection limit of the assay is at 0.2 nmol/L calculated from the maximum binding menus two standard deviations and the precision is satisfactory. In the recovery assay was used 4 milk different samples and the result was 98% of recuperation. The progesterone was determinate in milk samples from post-partum animals taking samples three times per week for 40 days. The assay is simple, rapid and possibility the progesterone measurement without sample dilution, distinguish the cyclic changes of this hormone that reflect the ovarian activity in the animals. (author)

  15. 放免定量检测分析中室内质量控制的探讨%Investigation and Practice of Internal Quality Control in Radioimmunoassay

    Institute of Scientific and Technical Information of China (English)

    余丽丽; 杨淑敏; 苏玉林; 张红; 王玥鸿

    2011-01-01

    Objective To investigate the application of internal quality control with two control materials in analysis of thyroid hormone by radioimmunoassay (RIA), in order to effectively reduce losing of control in testing results, by which to afford accurate and authentic test information for clinic. Methods Radioimmunoassay was used in this study. Non-fixed value control material which was self-regulated with batch number P20090723 and fixed value control materials from the three venders were used to carry on internal quality control in analysis of thyroid hormone for the working days in the year by RIA. Results The target value and standard uncertainty of fixed value control material was highly in accordance with the intermediate standard value of thyroid hormone. The analysis of the value in table 1 and table 2 demonstrated that there was statistic difference in mean and standard deviation between our laboratory and venders' (P < 0.05). Corclusions When analysing with any control material, a laboratory should establish its own target value and standard deviation for effective internal quality control, which can make the accurateness of every batch of test could be recurred by higher precision and accuracy. Our laboratory fixed the normal reference ranges of thyroid hormone with non-fixed value control material self-regulated.%目的 探讨放射免疫技术测定甲状腺激素分析中两种质控品在室内质量控制方法上的应用,有效地避免或减少发生项目检测结果失控的现象,为临床科室提供准确可信的实验报告.方法 采用自制的P20090723批号非定值质控品,以及三个厂家分别提供的6个甲功项目药盒内定值质控品,进行甲状腺功能6个项目全年250个工作日内部质量控制.结果 确定出自制非定值质控品在我室的中心线(靶值)和标准不确定度,非常符合甲功6项目标准范围的中间值,并认为250批次非定值质控晶源于同一总体.可以认为本实验室与

  16. Trace analysis of chloramphenicol residues in eggs, milk, and meat: comparison of gas chromatography radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Arnold, D.; Somgyi, A.

    A radioimmunological assay (RIA) to detect chloramphenicol (CAP) residues in eggs, milk, and meat is described. For tissues and other edible products of chloramphenicol-treated animals (chickens, cows, and pigs), the limit of detection is about 200 ng/kg. Residue levels above 1 ..mu..g/kg can easily be quantitated. When highly specific antisera produced in sheep were used, cross-reactivity was insignificant except for metabolites deviating from the parent compound in the acyl side chain only. Thiamphenicol fails to bind to the antisera; hence, it does not interfere with the assay. In the procedure described, the role of cleanup is merely to remove lipids. Thus, skim milk can be analyzed following appropriate dilution without cleanup. The results obtained by RIA were confirmed by gas chromatography with electron capture detection. The new RIA allows rapid, sensitive, and specific screening of large numbers of samples.

  17. Influence of molecular weight of DNA on the determination of anti-DNA antibodies in systemic lupus erythematosus (SLE) sera by radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Geisert, M.; Heicke, B.; Metzmann, E.; Zahn, R.K.

    1975-04-01

    Using a radioimmunoassay (RIA) based on the Farr technique with radioactively labeled /sup 3/H-DNA for quantitative measurements of anti-DNA antibodies in sera of patients with systemic lupus erythematosus (SLE), the influence of molecular weight of DNA (ranging from 0.1 x 10/sup 6/ to 22.0 x 10/sup 6/ daltons) on binding and precipitation in this system has been investigated. Comparing our results with mathematical models it follows that one antibody molecule is fixed on the average to a statistical DNA segment of 2 x 10/sup 6/ to 4 x 10/sup 6/ daltons. Furthermore binding capacity of the DNA was found to be independent of the molecular weight, as demonstrated in a double label experiment using /sup 14/C and /sup 3/H-labeled DNA of different size. However, the amount of radioactivity precipitated was found to depend on the molecular weight of the labeled DNA following a non-linear function. It was calculated that a minimal ratio of fixed antibody molecules per a certain size of DNA was necessary for precipitation. The mathematical treatment of the observed non-linear precipitation dependence will be discussed using various statistical models. The results indicate that the quantitative measurements of anti-DNA antibodies with the Farr technique e.g., for diagnosis and control of SLE in clinical immunology is highly dependent on the molecular weight of the labeled DNA used in the assay system and reliable results are only obtained with DNA of a sufficiently high molecular weight. (auth)

  18. Evaluation of a radioimmunoassay (/sup 125/I) kit for cannabinoid metabolites in urine and whole blood

    Energy Technology Data Exchange (ETDEWEB)

    Childs, P.S.; McCurdy, H.H.

    The Abuscreen kit (Roche Diagnostics) for the analysis of 11-nor-..delta../sup 9/-tetrahydrocannabinol-9-carboxylic acid and other cannabinoids in urine was evaluated in terms of its accuracy, reproducibility, and sensitivity. A procedure is also presented for the analysis of total cannabinoids in whole blood using the RIA kit.

  19. [Arylsulfatase A--physico-chemical properties and the use of enzyme radioimmunoassay in medical diagnosis].

    Science.gov (United States)

    Laidler, P M

    1991-01-01

    Arylsulfatase A was isolated from urine and human liver. The enzyme was homogeneous with respect to charge and had high specific activity--64 U/mg and 34 U/mg for arylsulfatase A from urine and liver respectively. The enzyme from urine as well as the liver one contained two nonidentical subunits with molecular weights varying about 5 kDa. Treatment of the enzyme from urine, liver and from placenta with endo-beta-N-acetylglucosaminidase F did not remove all carbohydrate from any subunit even in denaturing conditions. Deglycosylation of the enzyme with this one and other glycosidases under various conditions resulted in a decrease in the apparent molecular weights of subunits only by 1-2 kDa. The difference between molecular weights of subunits did not change upon deglycosylation of arylsulfatase A. The results suggest that the presence of two nonidentical subunits is due to presence of different polypeptides rather than various glycosylation of a single polypeptide chain. Arylsulfatase A from urine was inactivated following reaction with diethyl pyrocarbonate at pH 5.5 or at pH 7.0. This confirmed the presence of histidine essential for its catalytic activity. It was also shown that the enzyme was inactivated with ferrate ion, structural analogue of orthophosphate and strong oxidizing agent. The conditions of inhibition of arylsulfatase A carried out with the use of ferrate as well as catalytic and immunochemical properties of the modified enzyme suggest that ferrate reacted with the active site of arylsulfatase A. The results allow to expect that a reactive histidine is present in enzyme's active site and that this aminoacid is modified with ferrate. A simple, sensitive and specific radioimmunoassay was developed for the determination of arylsulfatase A in human serum and urine. The method allows to measure less than nanogram amounts of the enzyme in human body fluids. The test was used to determine arylsulfatase A in serum specimens of 368 patients with

  20. Coriorretinite esclopetária

    Directory of Open Access Journals (Sweden)

    Isaac Carvalho de Oliveira Ramos

    2015-06-01

    Full Text Available O objetivo deste trabalho é relatar um caso de trauma ocular por projétil de arma de fogo, que atingiu e se alojou na cavidade orbitária, desenvolvendo coriorretinite esclopetária. Foram abordados o mecanismo fisiopatológico, os principais achados clínicos e de exames complementares, além das opções de tratamento. As características do caso relatado reforçam a importância de uma abordagem multidisciplinar no trauma ocular.

  1. Radioimmunoassay for wheat gliadin to assess the suitability of gluten free foods for patients with coeliac disease

    Energy Technology Data Exchange (ETDEWEB)

    Ciclitira, P.J.; Ellis, H.J. (United Medical School of Guy' s and St. Thomas' Hospital, London (UK)); Evans, D.J. (Hammersmith Hospital, London (UK)); Lennox, E.S. (Medical Research Council, Cambridge (UK))

    1985-03-01

    Coeliac disease is a clinical condition characterised by malabsorption secondary to abnormalities of the small intestine. The condition is known to be exacerbated by wheat gliadin, rye, barley and possibly oats. The only assays that are available for testing for the presence of wheat gluten in foods are double diffusion against rabbit anti-gliadin antiserum and measurement of Kjeldahl nitrogen in products derived from wheat flour. We have developed a radioimmunoassay for wheat gliadin with a detection limit of 1 ng. Nominally gluten free foods based on wheat starch have been shown to contain up to 1.9 x 10/sup -2/% wheat gliadin. Bread made from Nutregen wheat starch which has now been withdrawn contains 6.4 mg gliadin per standard 30 g slice. A radioimmunoassay for wheat gliadin could be used to define standards for the suitability of gluten free products based on wheat starch for patients with coeliac disease.

  2. Neuropeptide Y and vasoactive intestinal peptide in experimental subarachnoid hemorrhage; Immunocytochemistry, radioimmunoassay and pharmacology

    Energy Technology Data Exchange (ETDEWEB)

    Alafaci, C.; Jansen, I. (Department of Experimental Research, Lund University, Malmoe General Hospital (Sweden)); Uddman, R. (Department of Otorhinolaryngology, Lund University, Malmoe General Hospital (Sweden)); Delgado, T.; Svendgaard, N.A. (Department of Neurosurgery, University Hospital, Lund (Sweden)); Edvinsson, L. (Department of Internal Medicine, University Hospital, Lund (Sweden)); Ekman, R. (Department of Psychiatry and Neurochemistry, University Hospital, Lund (Sweden))

    1991-01-01

    The involvement of noradrenaline (NA), neuropeptide Y, (NPY), 5-hydroxytryptamine (5-HT), acetylcholine (ACh) and vasoactive intestinal polypeptide (VIP) has been examined in the late phase of spasm after an experimental subarachnoid hemorrhage (SAH) in a rat model. Immunocytochemistry and radioimmunoassay of blood vessels from the circle of Willis did not show significant differences in NPY- and VIP-like immunoreactivity 2 days post SAH as compared to control vessels. The postjunctional effects of NA, NPY, 5-HT, Ach and VIP were studied two days after SAH using a sensitive in vitro system. NPY induced contractions were significantly (p<0.01) weaker (lower E{sub max}) in SAH as compared to control rats while the relaxant responses to ACh and VIP were slightly increased after SAH. These observations reveal that in a rat model of SAH, with an approximately 20% in vivo constriction at two days, dynamic changes occur in cerebral artery reactivity without any obvious change in sympathetic or parasympathetic perivascular nerve networks. (author).

  3. Radioimmunoassay of serum antibodies with B-streptococcus specificity in pregnant women and infants

    Energy Technology Data Exchange (ETDEWEB)

    Frey, C.W.

    1980-01-01

    In a specific competitive radioimmunoassay of purified rabbit antibodies, labeled with iodine 125 against group- and type-antigens of streptococcus agalactiae (streptococci type B), we investigated the amount of serum anti-bodies providing specificity of streptococci type B in not preselected pregnant women, newborn and babies with colonies of streptococci type B or with diseases due to streptococci type B and in some of their mothers. These antibodies could be detected in 26 of 45 pregnant women and in 3 of 7 children with colonies of streptococci type B. 5 of 18 newborn with the ''early-onset'' type of infection and 6 of 7 of their mothers provided antibodies with specificity of streptococci type B as did one of two newborn with the ''late onset'' type of infection. Contrary to the supposition of Baker and Kasper and in accordance with the findings of Wilkinson, the ''risk group'' cannot be determined only by detecting the antibodies against streptococci type B. The risk group comprises those persons in whom the colonisation of streptococci agalactiae leads to the frequently life-threatening infecton of neonatals with streptococci type B.

  4. Detection and specifity of class specific antibodies to whole bacteria cells using a solid phase radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Czerkinsky, C.; Rees, A.S.; Bergimeier, L.A.; Challacombe, S.J. (Guy' s Hospital Medical and Dental Schools, London (UK))

    1983-07-01

    A solid phase radioimmunoassay has been developed which can be used for the detection of isotype specific antibodies to whole bacteria and other particulate antigens, and is applicable to a variety of species. Bacteria are bound to the solid phase by the use either of antibodies, or of methyl glyoxal. Both methods result in a sensitive and reproducible assay, and bacteria do not appear to desorb from the solid phase. The specificity of antibodies to whole bacteria was examined by absorption of antisera with various species of bacteria and retesting, or by determining the binding of antisera to various bacteria bound to the solid phase. Both methods revealed specificity for the bacteria examined. Inhibition studies showed that antibodies to Streptococcus mutans whole cells could be inhibited by purified cell surface antigens glucosyltransferase and antigen I/II, but only minimally by lipoteichoic acid, c polysaccharide or dextran. In murine antisera antibodies of the IgG, IgM, and IgA classes could be detected at amounts of less than 1 ng/ml.

  5. Radioimmunoassay of arginine vasopressin in Rhesus Monkey plasma. [/sup 125/I tracer technique

    Energy Technology Data Exchange (ETDEWEB)

    Hayward, J.N.; Pavasuthipaisit, K.; Perez-Lopez, F.R.; Sofroniew, M.V.

    1976-04-01

    Using a new antiserum and an enzymatic radioiodination of arginine vasopressin (AVP), we have developed a sensitive and specific radioimmunoassay for plasma AVP in the monkey. The sensitivity of the assay is 0.5 ..mu..U/ml, the cross reaction with oxytocin (OT), minimal. We used this assay to study the effects that variations in blood osmolality have in regulating AVP secretion in unanesthetized, chair-restrained, chamber-isolated, adult female rhesus monkeys. Under water ad lib conditions, plasma AVP and osmolality were relatively constant, averaging 1.7 +- 0.6 (SD) ..mu..U/ml and 298 +- 3 mosmol/kg, respectively. Water loading decreased plasma AVP and osmolality to 0.6 +- 0.2 ..mu..U/ml and 282 +- 6 mosmol/kg, respectively. When fluid restriction increased osmolality, plasma AVP rose progressively to twice the baseline after 1 day, and to 6 times the baseline after 3 days. The rise in plasma AVP was linearly correlated with the rise in osmolality (r = 0.93; P less than 0.001). Intravenous infusions of hypertonic saline produced significant rises in plasma osmolality and plasma AVP. There was a dose-related rise in plasma AVP that declined later at the expected rate with the infusion of physiological amounts of synthetic AVP.

  6. Radioimmunoassay of buprenorphine in urine: Studies in patients and in a drug clinic

    Energy Technology Data Exchange (ETDEWEB)

    Hand, C.W.; Ryan, K.E.; Dutt, S.K.; Moore, R.A. (DPC European Research Institute, Witney (England)); O' Connor, J.; Talbot, D. (Jervis Street Hospital, Dublin (Ireland)); McQuay, H.J. (Abingdon Hospital, Oxford (England))

    A radioimmunoassay kit (DPC buprenorphine double antibody) was evaluated with clinical samples and samples from a drug clinic. Urine samples were collected over a 2-day period from 5 hospital in-patients receiving sublingual buprenorphine, 400 to 2000 {mu}g/day, for the relief of chronic pain. Samples were measured before and after enzymatic hydrolysis. Urine buprenorphine concentrations were measurable at all doses studied (minimum value 5.6 ng/mL) and were greater with larger doses. The increase in concentration after hydrolysis averaged 49% and was similar for all doses studied. The authors conclude that the method has extensive cross-reactivity with glucuronides of buprenorphine and its metabolites and that samples may be analyzed without prior hydrolysis. The prevalence of buprenorphine use in 97 patients attending a drug clinic was also studied. Sixty (62%) had measurable urinary buprenorphine concentrations of 1 ng/mL or more by direct assay. The buprenorphine users were significantly younger and reported significantly greater use of opiates than nonusers.

  7. Assessing dehydroepiandrosterone in saliva: a simple radioimmunoassay for use in studies of children, adolescents and adults

    Energy Technology Data Exchange (ETDEWEB)

    Granger, D.A.; Schwartz, E.B.; Booth, A.; Curran, M.; Zakaria, D. [Departments of Biobehavioral Health and Sociology, Behavioral Endocrinology Laboratory, The Pennsylvania State University, 315 E. Henderson Building, University Park, PA (United States)

    1999-07-01

    While salivary assays for some hormones are widely used, the availability of assays for salivary DHEA is limited. By adapting a commercially available radioimmunoassay serum kit, we developed a reliable, efficient and sensitive measure of DHEA in saliva that does not require separation or extraction. The minimum detection limit was 4.0 pg/ml. Intra-assay coefficients of variation (CV%) were on average 4.05, and inter-assay CVs averaged 9.70. Method accuracy, determined by spike recovery, and linearity, determined by serial dilution, averaged 99.55 and 92.03%. Levels in matched serum and saliva samples showed strong linear relationships for adult males and females. Specific guidelines are developed for sample collection, storage, and preparation procedures. Reference ranges for salivary DHEA levels are provided for 64 children ages 8-11, 96 adolescents ages 12-17 and 48 adults ages 30-45. Salivary DHEA levels are shown to reflect developmental, gender and diurnal differences. (Copyright (c) 1999 Elsevier Science B.V., Amsterdam. All rights reserved.)

  8. New sensitive serum melatonin radioimmunoassay employing the Kennaway G280 antibody: Syrian hamster morning adrenergic response.

    Science.gov (United States)

    Vaughan, G M

    1993-09-01

    A new procedure with the G280 antibody of Kennaway provides an assay for circulating melatonin (aMT) with a sample volume (200 microliters), an analytic (0.33 pg/ml) and functional (0.62-0.80 pg/ml) detectability, a 50% displacement dose (6.4 pg/ml), a Kd (0.657 pM), and measured circulating daytime levels lower than reported for previous procedures, and 100% assay recovery. The normal daytime range in adult human and Syrian hamster serum was 0.4-4 pg/ml. The pattern of fall of the nocturnal surge of Syrian hamster serum aMT near the time of lights-on was unaltered by extended darkness. Isoproterenol (ISO) injection 1 hr after lights-on, when aMT had reached daytime levels, raised serum and pineal aMT dramatically 2 hr postinjection. The same dose of ISO injected 4 hr into light produced only a small detectable increase. Novel extension of nocturnal darkness did not affect the responses to ISO. Thus, when they are allowed to occur at the usual time on a 10-hr dark schedule, both the fall from the nocturnal aMT surge and the subsequent loss of pineal beta-adrenergic responsiveness in this species occur endogenously (probably entrained) rather than from gating by acute effects of morning light. Changes in daytime serum aMT consistent with concomitant changes in the pineal can be measured with a sufficiently sensitive radioimmunoassay.

  9. Characterization and measurement of human apolipoprotein A-II by radioimmunoassay.

    Science.gov (United States)

    Goldberg, R B; Karlin, J B; Juhn, D J; Scanu, A M; Edelstein, C; Rubenstein, A H

    1980-09-01

    The development of a radioimmunoassay for apolipoprotein A-II (apo A-II) is described. Initial studies revealed a lack of immunological identity between purified apo A-II used as the standard and serum or HDL. Extensive testing of different buffers, standards, antisera, tracers, utilization of a detergent, and heating of sera failed to resolve the problem. Gel filtration of iodinated and non-iodinated apo A-II on Sephadex G-100 columns showed that apo A-II, in dilute solution, elutes in a higher molecular zone than expected with a broad, assymetrical profile. The use of a subfraction of the tracer in the assay resulted in parallelism in the serum and standard dilution curves. The apo A-II assay was sensitive, specific, and reproducible. Apo A-II added to sera was fully recovered and delipidation did not affect the immunoreactivity of either serum or HDL. Apo A-II contributed approximately 20% to the protein mass of HDL. Comparison of these results with those obtained by radial immunodiffusion, and with previously reported data, indicates that the reactivity of apo A-II in its native and delipidated forms may be markedly influenced by different immunologic methodologies and their specific reagents. Caution should thus be shown at present in assigning absolute concentrations to apo A-II in serum or HDL.

  10. Cross-reactivity of amphetamine analogues with Roche Abuscreen radioimmunoassay reagents

    Energy Technology Data Exchange (ETDEWEB)

    Cody, J.T. (Air Force Drug Testing Laboratory, Brooks AFB, TX (USA))

    1990-01-01

    Cross-reactivity of amphetamine analogues with the Abuscreen amphetamine radioimmunoassay reagents was determined for both the standard and high specificity antibody systems. Compounds tested included 2-methoxyamphetamine, 4-hydroxymethamphetamine, 2,5-dimethoxyamphetamine (DMA), 4-bromo-2,5-dimethoxyamphetamine (DOB), 4-bromo-2,5-dimethoxy-beta-phenethylamine (BDMPEA), 3,4,5-trimethoxyamphetamine (TMA), 3,4-methylenedioxyamphetamine (MDA), N,N-dimethyl-3,4-methylenedioxyamphetamine and N-hydroxy-3,4-methylenedioxyamphetamine (N-OH MDA), 3,4-methylenedioxymethamphetamine (MDMA), 3,4-methylenedioxyethylamphetamine (MDEA), 2,5-dimethoxy-4-ethylamphetamine, 2,5-dimethoxy-4-methylamphetamine (DOM), and 3,4,5-trimethoxyphenethylamine (mescaline). Blank negative reference material was spiked with 1,000 to 100,000 ng/mL of the amphetamine analogue and used as sample in the assays. MDA was the only analogue that showed cross reactivity equal to or greater than that of amphetamine. None of the other analogue compounds demonstrated a positive result at even the highest concentration; however several showed depressed counts at various concentration levels.

  11. Atrial natriuretic factor: radioimmunoassay and effects on adrenal and pituitary glands

    Energy Technology Data Exchange (ETDEWEB)

    Gutkowska, J.; Horky, K.; Schiffrin, E.L.; Thibault, G.; Garcia, R.; De Lean, A.; Hamet, P.; Tremblay, J.; Anand-Srivastava, M.B.; Januszewicz, P.

    1986-06-01

    A simple and sensitive radioimmunoassay was developed for measurement of immunoreactive atrial natriuretic factor (IR-ANF) in rat and human plasma and in rat atria. The two atria contain about 20 ..mu..g ANF per rat. The right atrium contained 2.5 times more ANF than did the left. Ether anesthesia and morphine markedly increased IR-ANF in rat plasma. The concentration of IR-ANF in plasma of clinically normal human subjects was 65.3 +/- 2.5 pg/ml. Paroxysmal tachycardia and rapid atrial pacing significantly increased IR-ANF in human plasma. Two- to seven-fold higher concentrations were found in coronary sinus blood than in the peripheral circulation. In the plasma of rats and humans, circulating ANF is probably a small-molecular-weight peptide. ANF acts on the adrenal and the pituitary. ANF inhibits aldosterone secretion from rat zona glomerulosa and steroid secretion by bovine adrenal zona glomerulosa and fasciculata. ANF stimulated the basal secretion of arginine vasopressin (AVP) in vitro and inhibited KCl-stimulated release of AVP.

  12. Alcohols which have been in contact with any plastics may interfere in radioimmunoassays of progesterone.

    Science.gov (United States)

    Ocvirk, Rok; Bisson, Jennifer M; Murphy, Beverley E Pearson

    2009-01-01

    In recent years there has been increasing use of plastic rather than glass containers for many liquids, including wine. However we have found that residue from commercially obtained 'pure' ethanol dispensed in plastic bottles interferes in some biochemical assays. We have observed a volume-dependent decrease in maximally bound ligand in radioimmunoassays of progesterone. The resulting shift in the standard curve leads to an underestimation of the analyte concentrations and to altered estimation of cross reactivity by competing ligands. These effects became apparent in assays with high sensitivity (500 pg or less). All sources of ethanol obtainable in Quebec contained impurities. A similar effect was also produced by 'pure' methanol. The reduction in maximally bound ligand was amplified when the alcohol was aliquoted using plastic pipette tips. We conclude that alcohols which have had any contact with plastics are not safe to use in immunoassays of progesterone (or its metabolites as estimated according to cross-reactivity after HPLC) and may affect other assays. If the use of alcohol and plastic tips cannot be avoided, the amount of alcohol used should be reduced to 1% or less. This can be accomplished by preparing steroid standards in assay buffers containing albumin or gelatin, which enhance the solubility of steroids in aqueous media.

  13. Selective and simplified radioimmunoassay of 25-hydroxyvitamin D/sub 3/

    Energy Technology Data Exchange (ETDEWEB)

    Hummer, L.; Nilas, L.; Tjellesen, L.; Christiansen, C. (Glostrup Sygehus, Copenhagen (Denmark))

    1984-04-01

    A simple, non-chromatographic and selective assay for determination of 250 HD/sub 3/ in serum is described. The serum sample is deproteinated with acetonitrile and the supernatant purified over a small prepacked cartridge, Sep-pak. In the eluate from the cartridge, 250 HD/sub 3/ is measured by radioimmunoassay. The selectivity of the assay for 250 HD/sub 3/ is improved by adding vitamin D/sub 2/ to the antiserum; the assay is thus capable of measuring 250 HD/sub 3/ in serum samples containing up to a ratio of 250 HD/sub 2//250 HD/sub 3/ equal to 150. The simplicity, speed and the small amount of sample needed (0.5 ml) make this method suitable for use in a routine clinical laboratory. Because of its selectivity for 250 HD/sub 3/ the assay is a necessary prerequisite in the further investigation of metabolic discrimination between vitamins D/sub 2/ and D/sub 3/.

  14. Sensitive and simplified radioimmunoassay for 1,25-dihydroxyvitamin D/sub 3/

    Energy Technology Data Exchange (ETDEWEB)

    Scharla, S.; Schmidt-Gayk, H.; Reichel, H. (Heidelberg Univ. (Germany, F.R.). Chirurgische Universitaetsklinik); Mayer, E. (Heidelberg Univ. (Germany, F.R.). Medizinische Poliklinik)

    1984-10-15

    A sensitive radioimmunoassay system for 1..cap alpha..,25-dihydroxyvitamin D/sub 3/ (1,25(OH)/sub 2/D/sub 3/) with an improved extraction procedure has been developed. Following one-step extraction and prepurification of 1,25(OH)/sub 2/D/sub 3/ by 'Extrelut-1' minicolumns final purification was achieved by high-performance liquid chromatography (HPLC) using a radial compression separation system equipped with a ..mu..Porasil cartridge. Recovery of 1,25(OH)/sub 2/(/sup 3/H)D/sub 3/ after HPLC was 77+-2.6% (mean +- SD, n = 51). The sensitivity of the assay was 0.8 pg/tube resulting in a detection limit of 3 ng/l, when 1 ml of serum was extracted. Intra-assay and inter-assay coefficients of variation were 12% and 16.8%, respectively. Serum 1,25(OH)/sub 2/D/sub 3/ concentration in 30 normal subjects was 55 +- 12 ng/l (mean +- SD). Patients with chronic renal failure had reduced 1,25(OH)/sub 2/D/sub 3/ serum levels (mean 5.4 ng/l, range 3-11 ng/l, n=10).

  15. Serum and urinary oestrone sulphate in pregnancy and delivery measured by a direct radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Honjo, Hideo; Kitawaki, Jo; Itoh, Masafumi; Yasuda, Jinsuke; Yamamoto, Takara; Yamamoto, Takao; Okada, Hiroji; Ohkubo, Tadashi; Nambara, Toshio

    1986-01-01

    Serum and urinary levels of oestrone sulphate in pregnancy and delivery were measured by a direct radioimmunoassay without hydrolysis. the serum and urinary oestrone sulphate increased as pregnancy progressed. the mean level of serum oestrone sulphate increased to the highest peak of 494 pmol/ml at the 35th gestational week and then decreased. The mean level of urinary oestrone sulphate increased to the highest peak of 1.28 ..mu..mol/l at the 34th gestational week and the decreased. At vaginal deliveries, the mean level of maternal peripheral serum oestrone sulphate increased hourly at as high a level as 979 pmol/ml. The mean serum level of oestrone sulphate was 204 pmol/ml in the umbilical artery and 145 pmol/ml in the umbilical vein. At Caesarean section, on the other hand, the maternal peripheral serum level of oestrone sulphate averaged 362 pmol/ml. The mean serum levels of oestrone sulphate wre 90.7 pmol/ml and 171 pmol/ml in the umbilical artery and umbilical vein, respectively. These results suggest a maternal origin of oestrone sulphate in pregnancy, with fluctuations in the levels being of interest in relation to labour pain.

  16. Development of a specific radioimmunoassay to measure physiological changes of circulating leptin in cattle and sheep.

    Science.gov (United States)

    Ehrhardt, R A; Slepetis, R M; Siegal-Willott, J; Van Amburgh, M E; Bell, A W; Boisclair, Y R

    2000-09-01

    Studies of leptin in large domestic ruminants have been limited to measurements of gene expression because methods to measure circulating levels are not available. To develop a bovine leptin radioimmunoassay, we produced recombinant bovine leptin and used it to immunize rabbits, and to prepare bovine leptin tracer and standards. A single antiserum with sufficient affinity and titer was identified. Using this antiserum, logit-transformed binding of (125)I-labeled bovine leptin was linearly related (R(2)= 0.99) to the log of added bovine or ovine leptin between 0.1 to 2.0 ng. Serial dilution of bovine and ovine plasma, chicken serum and bovine milk gave displacement curves that were parallel to those of bovine or ovine leptin. Recoveries of external addition of bovine leptin in ewe and cow plasma ranged between 94 and 104%. Plasma leptin concentration measured by this assay was directly related to the plane of! nutrition in growing calves and lambs. At 11-14 weeks of age, ewe lambs had a higher circulating leptin concentration than ram lambs. Finally, plasma leptin concentration was linearly related to the fat content of the empty carcass in growing cattle and to body condition score in lactating dairy cows. We conclude that circulating leptin in sheep and cattle is increased by fatness and plane of nutrition, consistent with results in humans and rodents. This assay provides an important tool to investigate mechanisms that regulate plasma leptin in cattle and sheep.

  17. Formation and distribution of sennosides in Cassia angustifolia, as determined by a sensitive and specific radioimmunoassay.

    Science.gov (United States)

    Atzorn, R; Weiler, E W; Zenk, M H

    1981-01-01

    A radioimmunoassay for the quantitation of nanogram-amounts of sennoside B and related compounds in plant extracts is described. The assay makes use of [ (3)H]-8-glucosidorheinanthrone of high specific activity (5.2 Ci/mmol) whose synthesis is reported here. From this material, [ (3)H]-sennoside A and [ (3)H]-sennoside B have also been synthesized. The assay is applied to the analysis of sennoside formation and distribution in CASSIA ANGUSTIFOLIA VAHL. High levels of sennosides in dried leaves and fruits have been observed whereas the seed alone, as well as stems and roots, contain very little sennoside. In flowers, as much as 4-5% of the dry weight consists of sennoside B and other immunoreactive constituents. Sennosides have been found in cotyledons of three day old seedlings in concentrations comparable to that of the mature leaf. Upon dehydration, leaf levels of sennoside B rise steadily, this rise being inversely correlated with the water loss. The absolute levels of sennoside B formed this way are the same as compared to rapid drying at 60 degrees C.

  18. Radioimmunoassay for anileridine, meperidine, and other N-substituted phenylpiperidine carboxylic acid esters

    Energy Technology Data Exchange (ETDEWEB)

    Van Vunakis, H.; Freeman, D.S.; Gjika, H.B.

    1975-10-01

    Antibodies that bind an /sup 125/I-tyramyl derivative of N-succinylanileridine have been produced in animals immunized with N-succinylanileridine-hemocyanin conjugate. Several congeners and metabolites have been tested as competitors of this antigen-antibody reaction. The concentrations (in picomoles) required for 50 percent inhibition have been found to be: anileridine (0.2), meperidine (3.5), piminodine (3.8), diphenoxylate (20.5), normeperidine (20.0), meperidine acid (45,000) and anileridine acid (3,400). Although ester hydrolysis results in changes in inhibiting capacities on the order of 10/sup 4/, major structural changes in the substituent on the nitrogen of the piperidine ring are not readily recognized by the antibody. This radioimmunoassay can be used to study a variety of N-substituted phenylpiperidine carboxylic acid esters by relating the results to the standard curve obtained for the drug under investigation. For all practical purposes, alphaprodine, morphine and methadone do not interfere with the assay.

  19. Retrospective evaluation of the Du Pont radioimmunoassay kit for detection of Legionella pneumophila serogroup 1 antigenuria in humans.

    OpenAIRE

    Aguero-Rosenfeld, M E; Edelstein, P H

    1988-01-01

    We used the Du Pont radioimmunoassay kit for soluble Legionella pneumophila serogroup 1 antigenuria (Du Pont Co., Wilmington, Del.) to test 422 urine samples from patients with and without Legionnaires disease (LD). The urine specimens were collected from 23 patients with culture-proven LD and from 346 patients without LD. L. pneumophila serogroup 1 was isolated from 14 patients with culture-proven LD, and other L. pneumophila serogroups or other Legionella species were isolated from 9 patien...

  20. Assay of mouse-cell clones for retrovirus p30 protein by use of an automated solid-state radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Kennel, S.J.; Tnnant, R.W.

    1979-09-01

    A solid-state radioimmunoassay system has been developed that is useful for automated analysis of samples in microtiter plates. Assays for interspecies and type-specific antigenic determinants of the C-type retrovirus protein, p30, have been used to identify clones of cells producing this protein. This method allows testing of at least 1000 clones a day, making it useful for studies of frequencies of virus protein induction, defective virus production, and formation of recombinant viruses.

  1. A rapid radioimmunoassay using /sup 125/I-labeled staphylococcal protein A for antibody to varicella-zoster virus

    Energy Technology Data Exchange (ETDEWEB)

    Richman, D.D.; Cleveland, P.H.; Oxman, M.N.; Zaia, J.A.

    1981-05-01

    A sensitive radioimmunoassay for serum antibody to varicella-zoster virus is described; it uses 125I-labeled staphylococcal protein A and a specially designed immunofiltration apparatus. The assay accurately distinguishes between individuals who are susceptible and those who are immune to infection with varicella-zoster virus. In addition, it can detect passive antibody in recipients of varicella-zoster immune globulin. This radioimmunoassay also detects the heterologous antibody responses that occasionally occur in patients infected with herpes simplex virus, which also have been detected by other antibody assays. The particular advantages of this assay are the use of noninfectious reagents, the speed of execution (less than 3 hr), the requirement for only small quantities of serum (30 microliters), the objectivity of end-point determination, and the capability of screening large numbers of sera. Consequently, this radioimmunoassay is especially useful for the rapid identification of susceptible individuals, which is essential for the appropriate management of patients and hospital personnel after exposure to varicella.

  2. Stereospecific antibodies to methadone. I. Radioimmunoassay of d,l-methadone in human serum.

    Science.gov (United States)

    Bartos, F; Olsen, G D; Leger, R N; Bartos, D

    1977-01-01

    Anti-d,l-methadone antibodies were produced in rabbits immunized with d,l-methadol-hemisuccinate thyroglobulin conjugate. Using the antiserum, a radioimmunoasay (RIA) for determination of d,l-methadone in human serum has been developed and is described. Concentration of d,l-methadone of 1.4 pmol in a native serum sample (volume 0.1 ml or less) could be measured directly by RIA. The antibodies crossreact 100% with d,l-methadone, 50% with d-methadone, 50% with l-methadone and 100% with alpha-d-methadol. No crossreactivity was found with alpha 1-methadol, morphine, meperidine, dextropropoxyphene, 2-ethyl-5-methyl-3,3-diphenyl-l-pyrroline and 2-ethylidene-l, 5-dimethyl-3,3-diphenylpyrrolidene. High sensitivity and small sample requirements make this method suitable for future monitoring of patients on methadone maintenance and for studies where other procedures have lack of sensitivity.

  3. Presence by radioimmunoassay of a calcitonin-like substance in porcine pituitary glands

    Energy Technology Data Exchange (ETDEWEB)

    Catherwood, B.D.; Deftos, L.J.

    1980-06-01

    We studied acidic acetone extracts of whole porcine pituitary glands for the presence of immunoreactive calcitonin (CT) using a porcine CT (pCT) RIA which did not react with other known pituitary hormones. Four preparations of porcine pituitary extract contained immunoreactive CT. Three of these displayed inhibition of binding parallel to that of authentic pCT in the pCT RIA and contained a single peak of immunoreactivity similar to pCT when studied by two different gel filtration chromatography systems. One preparation of porcine pituitary extract showed nonparallelism in RIA dose-dilution experiments and multiple immunoreactive species both similar to and larger than pCT on gel filtration in 6 M guanidine HCl. The effect of the reduction of disulfide bonds, followed by carboxymethylation of sulfhydryl groups, on immunoreactivity and apparent molecular size was similar for the CT-like substance in porcine pituitary extract and for authentic pCT. Preliminary immunohistological studies showed cytoplasmic staining in cells of the porcine adenohypophysis. These results demonstrate that the porcine pituitary gland contains a substance which has some of the immunochemical and biochemical properties of thyroidal pCT.

  4. Do bullying ao cyberbullying: hist?ria e mem?rias escolares (1993-2011)

    OpenAIRE

    Santos, Silv?nia da Silva

    2015-01-01

    O presente estudo, Do bullying ao cyberbullying: hist?ria e mem?rias escolares (1993-2011) ? uma proposta de investiga??o que contempla a hist?ria e as mem?rias de acontecimentos escolares do tempo presente. Nesse contexto, busca recriar fatos e acontecimentos de nosso tempo, representa??es do momento vivido ou imaginado, como forma de construir a mat?ria hist?rica. A investiga??o utiliza a metodologia da Hist?ria Oral, apropriando-se das mem?rias de participantes vivos, depoentes em potencia...

  5. Exposure to environmental tobacco smoke measured by cotinine sup 125 I-radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Knight, G.J.; Palomaki, G.E.; Lea, D.H.; Haddow, J.E. (Foundation for Blood Research, Scarborough, ME (USA))

    1989-06-01

    We describe a polyclonal-antiserum-based {sup 125}I-radioimmunoassay for cotinine that is suitable for measuring nonsmokers' passive exposure to tobacco smoke in the environment. The standard curve ranged from 0.25 to 12.0 micrograms/L, with an estimated lower limit of sensitivity of 0.2 microgram/L (95% B/Bo = 0.2 microgram/L; 50% B/Bo = 4.0 micrograms/L). The median within-assay CVs for patients' samples with cotinine values from 0.4 to 1.3, 1.4 to 2.4, 2.5 to 4.6, and 4.7 to 15.6 micrograms/L were 13.9%, 7.2%, 5.1%, and 5.7%, respectively. Between-assay CVs for two quality-control sera with average values of 1.53 and 3.68 micrograms/L were 14.3% and 7.8%, respectively. Analytical recoveries of cotinine from smokers' sera diluted in zero calibrant ranged from 91% to 116%. Cotinine values determined on 79 paired sera and urines from nonsmokers showed significant correlation with self-reported exposure to environmental tobacco smoke (r = 0.49, P less than 0.001 for sera; r = 0.57, P less than 0.001 for urine). The log of the values for serum and urine cotinine were also significantly correlated (r = 0.85, P less than 0.001). Evidently, polyclonal antiserum can be used to develop a cotinine assay for measuring exposure to environmental tobacco smoke that compares well with that described for monoclonal-based assays.

  6. Radioimmunoassay of (8-arginine)-vasopressin. II. Application to determination of antidiuretic hormone in urine.

    Science.gov (United States)

    Merkelbach, U; Czernichow, P; Gaillard, R C; Vallotton, M B

    1975-11-01

    A radioimmunoassay for [8-arginine]-vasopressin (AVP), previously described (Czernichow et al. 1975) has been used for the determination of antidiuretic hormone in a 4 ml urine sample. AVP is extracted from acidified urine with a cation exchanger (Amberlite CG 50) with an overall recovery of 72%. The blank value measured in extracted samples of urine was 0.29 pg/ml +/- 0.21 (SEM) and calculated by extrapolation of the regression line of the recovery experiment was 0.49 pg/ml. The coefficient of variation within-assay was 13% and between-assay 18%. Addition of the amounts of AVP found in each specimen of urine voided gave results nearly identical to those of the amounts found in 24 h pool of urine, indicating that the assay was not affected by changes in concentration of the other urinary components during the day. The daily urinary excretion of AVP measured in 34 subjects was found to be 34 ng in 17 women and 70 ng in 17 men, a significant difference. Urinary concentration and excretion rate of AVP rose during thirst test and during Carter-Robbins test performed in 13 healthy subjects. In the latter test it was observed that the women displayed a strikingly more pronounced AVP elevation after the osmolar stimulus than the men. In both sexes a significant correlation was found between AVP excretion rate and plasma osmolality as well as free water clearance. Three cases of complete or incomplete diabetes insipidus and potomania could be clearly differentiated according to the total output of AVP during the thirst test. Extremely high values of AVP were found in the urine of 5 subjects with Schwartz-Bartter syndrome associated with bronchogenic tumours.

  7. [Studies on development of radioimmunoassay for diazepam and it's medicolegal application].

    Science.gov (United States)

    Tomii, S

    1989-05-01

    A sensitive radioimmunoassay was developed for the determination of diazepam. Detection of diazepam in tissues of rats and in blood from unnatural death victims and traffic accident victims was examined by this procedure. Antisera capable of binding [3H] diazepam were obtained by repeated immunization of rabbits with temazepam (oxydiazepam)-3-hemisuccinate conjugated to bovine serum albumin. The antibody of a 1: 10000 dilution could bind approximately 50% of the added radioactivity. The level at least as low as 1 pg of diazepam could be detected by this procedure. This antibody strictly recognized the existence of the methyl group at the position-1 and of the keto group at the position-2 of the benzodiazepine ring, and was highly specific for temazepam and diazepam among benzodiazepine derivatives. But other benzodiazepine derivatives had no cross-reactivity with this antibody. The distribution of diazepam in biological fluids and tissues of rats 1, 2, 4 and 8 hr after intraperitoneal injection was examined. The level of diazepam in serum, saliva, brain and bone marrow declined within 2 hr, while the level in liver and kidney was maximum 2 hr after administration. The concentration of diazepam in each tissue leveled off 4 hr after administration. Among these results the remarkable finding was that the concentration in the bone marrow is much higher than that in serum, saliva and brain, appearing to be due to a high accumulation of diazepam in this tissue. This result indicates that the bone marrow is a very useful material for the detection of diazepam in skeletonized remains. There ware good correlations (gamma = 0.8595-0.9973) in the concentrations of diazepam between serum, saliva and brain, and bone marrow. Detection of diazepam in blood from unnatural death victims and traffic accident victims were examined. Diazepam was identified in 3 cases of 50 unnatural death victims and in 4 cases of 48 traffic accident victims.

  8. Electrophoretic purification of radioiodinated follicle-stimulating hormone for radioligand receptor assay and radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Schneyer, A.L.; Sluss, P.M.; Bosukonda, D.; Reichert, L.E. Jr.

    1986-10-01

    A method is described for electrophoretic purification of (/sup 125/I)human (h) FSH after radioiodination that improves radioligand binding to FSH membrane receptors. Lactoperoxidase-iodinated hFSH was separated from reaction products by electrophoresis on 7.5% polyacrylamide tube gels (PAGE). Material eluted from 3-mm gel slices was analyzed for incorporation of /sup 125/I and binding to antibody (RIA) or receptor (RRA), and by sodium dodecyl sulfate-PAGE for protein composition. Sodium dodecyl sulfate-PAGE analysis of individual PAGE fractions demonstrated that iodinated proteins, both higher and lower in apparent mol wt than intact FSH, were separated by PAGE, but not by gel filtration chromatography (Sephadex G-25). PAGE purification of radioligand resulted in significantly greater (compared to gel filtration) RRA sensitivity and specificity. Maximum binding of PAGE-purified (/sup 125/I)hFSH to excess calf tests membrane receptors was 45%, with a specific activity of approximately 26 microCi/micrograms, as determined by the method of self-displacement. Maximum binding to excess hFSH antisera (NIH anti-hFSH 4) was 80-85%. This allowed a useful final dilution of 1:120,000, thereby facilitating development of a sensitive and specific RIA with this antiserum. These data indicate that PAGE separation of intact (/sup 125/I)hFSH from other iodinated proteins results in improved radioligand binding, assay sensitivity, and assay specificity. In addition, PAGE-purified lactoperoxidase-iodinated hFSH is suitable for use in both RIA and RRA.

  9. Anemias Hereditárias

    OpenAIRE

    Ferreira, Fátima; Carvalho, Fernanda; Costa, Vítor; Farinha, Nuno; Gil-da-Costa, M.ª João; Norton, Lucília; Reis, Ilidia Lima

    2014-01-01

    Os autores efectuam a revisão das anemias hereditárias da consulta de hematologia pediátrica do Hospital S. João no período compreendido entre 1982 e 1995. Dividem-nas em três grandes grupos: Hemoglobinopatias, Doenças da Membrana do glóbulo rubro e Enzimopatias. Calculam a sua frequência relativa, os parâmetros clínicos e analíticos relevantes no diagnóstico, a abordagem terapêutica e respectiva evolução.Destacam a especificidade desta consulta em que as anemias hereditárias correspondem a 4...

  10. Fundamental and clinical evaluation of vasoactive intestinal peptide (VIP) in pancreatitis by radioimmunoassay kit

    Energy Technology Data Exchange (ETDEWEB)

    Uehara, Soichiro; Nakamura, Takayuki; Ihara, Kenichi; Isogawa, Shin; Hirayama, Akio

    1987-07-01

    Plasma vasoactive intestinal peptide (VIP) concentrations of normal individuals and patients with pancreatitis were studied using a VIP RIA kit. The inter-assay and intra-assay variation of this kit were between 2.1 and 9.4 %. The VIP levels increased in the acute phase of acute pancreatitis and patients with chronic pancreatitis. The VIP concentration increased during the first 30 min of glucose tolerance test, but this increase was much smaller than that in insulin. These results suggest that this kit is useful for physiologic and pathologic changes in the VIP level.

  11. Estrutura & História.

    Directory of Open Access Journals (Sweden)

    Alicia Ferreira Gonçalves

    2015-06-01

    Full Text Available O presente artigo discorre sobre as ligações entre estrutura e história a partir das perspectivas estruturalista francesa e culturalista norte-americana, focalizando as interlocuções teóricas entre Claude Lévi-Strauss e Marshall Sahlins entre o final dos anos 1940 e ao longo dos anos 1990 do século XX.

  12. Comparison of acid ethanol extraction and acid gel filtration prior to IGF-I and IGF-II radioimmunoassays; Improvement of determinations in acid ethanol extracts by the use of truncated IGF-I as radioligand

    Energy Technology Data Exchange (ETDEWEB)

    Bang, P.; Eriksson, U.; Wivall, I.-L.; Hall, K. (Department of Endocrinology, Karolinska Institute, Stockholm (Sweden)); Sara, V. (Department of Pathology, Karolinska Institute, Stockholm (Sweden))

    1991-01-01

    Insulin-like growth factor binding proteins interfere in the IGF-I and -II radioimmunoassays. In an attempt to overcome this problem, we have compared the use of truncated IGF-I, with reduced IGFBP affinity, and IGF-I as radioligands for IGF-I RIA measurements in serum separated by acid gel filtration or acid ethanol extraction followed by cryo-precipitation. With truncated IGF-I as radioligand the IGF-I measurements in acid gel filtrates and acid ethanol extracts were significantly correlated in healthy subjects (N=42, r=0.91, p<0.001) and in patients with acromegaly (N=10, r=0.85, p<0.01), GH deficiency (N=10, r=0.88, p<0.001) or Type I diabetes mellitus (N=10, r=0.90, p<0.001). In contrast, the IGF-I concentrations in acid ethanol extracts determined with IGF-I as radioligand did not correlate with those in acid gel filtrates using truncated IGF-I radioligand in patients with acromegaly (r=0.61, NS) or GH deficiency (r=0.46, NS). In the latter group the mean IGF-I concentrations measured in acid ethanol extracts were erroneously elevated by 112%. Low-affinity antibodies used for IGF-II RIA determinations failed to give reliable results in acid ethanol extracts from patients with Type I diabetes mellitus or GH deficiency. In conclusion, erroneously high IGF-I concentrations owing to binding of the radioligand to IGFBPs not completely removed by acid ethanol extraction can be avoided by the use of truncated IGF-I as radioligand. (author).

  13. Determination of trenbolone in urine and faeces of calves by radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Schopper, D.

    Radioimmunological methods were established for the determination of residues of trenbolone acetate (TBA) in urine and faeces of calves treated with TBA. Both methods are easy to handle (about 60-80 samples within 2 days). Their reliability has been demonstrated by means of examining specificity, sensitivity, accuracy and reproducibility. The lower limit of sensitivity is around 0.024-0.032 ng TBOH/ml urine and 0.034-0.048 ng TBOH/g faeces. Both RIAs could be applied for routine control of illegal treatment of veal calves with TBA. For the first time the excretion of TBOH after implantation with TBA was measured radioimmunological in 2 veal calves. Residues of TBA are measurable in urine and in faeces during the whole fest period (up to 61 days after implantation). The major part of TBOH is excreted with the faeces. Until the substances needed for the RIA of TBOH are available commercially, thin-layer-chromatography (Vogt 1977) can alternatively be used for the determination of residues of TBA.

  14. Radioimmunoassay method suitable for analysis of salivary cortisol in patients with breast cancer in treatment by teletherapy - a preliminary study

    Energy Technology Data Exchange (ETDEWEB)

    Souza, Kellen Verena S. [Departamento de Engenharia Nuclear. Universidade Federal de Minas Gerais, Pampulha, MG (Brazil); Santos, Jane Lima dos [Departamento de Ciencias Biologicas, Universidade Estadual de Santa Cruz, Ilheus, BA (Brazil)

    2011-07-01

    Determination of salivary cortisol using radioimmunoassay and the relationship between stress levels were investigated before and after of the psychological intervention of 21 patients during teletherapy treatment of breast cancer. The benefits of psychological intervention during cancer treatment has been observed in some studies and evidence show that the patient's emotional state directly influences adherence to treatment, good prognosis and under stress. Thus, in present study 21 patients aged 50-70 years, females in the treatment of breast cancer at the Radiotherapy Service of Santa Casa de Misericordia in Belo Horizonte, were divided randomly into two groups: control (without psychological intervention) and Group TCC (with psychological intervention). Salivary cortisol concentrations are reportedly about 5.10% of those in serum, the levels has been used as an objective marker of hypothalamic-pituitary-adrenal (HPA) axis activity and avoided the confounding effects of venepuncture stress on cortisol levels. The assessment of cortisol in saliva has proven a valid and reliable measure of unbound steroid in the circulation. The salivary cortisol measured by radioimmunoassay in control patients and TCC showed no significant differences. The radioimmunoassay method show be suitable for analysis of salivary cortisol because allows measure low detection limits and have high sensibility. The values of TCC group and Control group were higher than reference values, except for one patient of the control group. At the beginning of treatment, both groups were homogeneous for Teletherapy. Both the TCC group as the control group had . 80% of participants in phase with stress. It was observed in the TCC group after radiotherapy showing a reversal 60% of participants without stress. The data show that cognitive-behavioral therapy has benefits for the control of psychological stress and enhance relevance of the psychologist to provide reduction or stress control during

  15. Direct radioimmunoassay for estriol-16-glucuronide in urine for monitoring pregnancy and induction of ovulation. [Tritium tracer techniques

    Energy Technology Data Exchange (ETDEWEB)

    Haning, R.V. Jr.; Satin, K.P.; Lynskey, M.T.; Levin, R.M.; Speroff, L.

    1977-08-01

    Antibodies to estriol-16..cap alpha..-(..beta..-D-glucuronide) were raised in sheep with the use of keyhole limpet hemocyanin and bovine serum albumin conjugates of estriol-16..cap alpha..-(..beta..-D-glucuronide). A simple, rapid method is presented for direct radioimmunoassay of estriol-16..cap alpha..-(..beta..-D-glucuronide) in urine with dextran-coated charcoal used for separation of free from bound and deionized water used for dilutions. The method is thrifty in its use of reagents. The assay has been evaluated in the pregnancy range, and the sensitivity has been extended into the range necessary for monitoring induction of ovulation with pergonal.

  16. Radioimmunoassay of dermorphin-like peptides in mammalian and non-mammalian tissues.

    Science.gov (United States)

    Negri, L; Melchiorri, P; Erspamer, G F; Erspamer, V

    1981-01-01

    A selective RIA for D-Ala2-Dermorphin (Der), a natural peptide extracted from amphibian skin, has been developed using an antibody raised in rabbits against Der which has been coupled to BSA through its phenolic hydroxyl groups of tyrosine residues with 2,4-Dichloro-6-methoxy-1,3,5-triazine. The cross-reactivity of this antibody with dermorphin analogs, C- and N-terminal fragments of dermorphin molecule, some opioid and gastrointestinal peptides was tested. Der-like immunoreactivity has been identified in tissue extracts of rats, frog and cephalopoda. Der-like peptides were purified by passing methanol extracts of the tissues through a Sephadex G25 column (16 x 100 cm) eluted with 0.1 M acetic acid at 4 degrees C. Der-like immunoreactivity from neural tissue of Dosidicus gigas, Eledone moscata, and rat brain showed a good agreement with an authentic sample of synthetic dermorphin.

  17. Urticária Urticaria

    Directory of Open Access Journals (Sweden)

    Paulo Ricardo Criado

    2005-12-01

    Full Text Available A urticária apresenta-se com diversas formas clínicas e causas distintas. Constitui uma das dermatoses mais freqüentes: 15% a 20% da população têm pelo menos um episódio agudo da doença em sua vida, resultando em percentual que varia de um a 2% dos atendimentos nas especialidades de Dermatologia e Alergologia. A urticária é classificada do ponto de vista de duração da evolução temporal em aguda (inferior a seis semanas ou crônica (superior a seis semanas. O tratamento da urticária pode compreender medidas não farmacológicas e intervenções medicamentosas, as quais são agrupadas em tratamentos de primeira (anti-histamínicos, segunda (corticosteróides e antileucotrienos e terceira linha (medicamentos imunomoduladores. As medidas terapêuticas de segunda e terceira linha apresentam maiores efeitos adversos, devendo ser reservadas aos doentes que não apresentaram controle da doença com os de primeira linha, ou àqueles a respeito dos quais não é possível estabelecer uma etiologia, tal como nas urticárias auto-imunes.Urticaria has diverse clinical presentations and causes. It is one of the most frequent dermatological conditions: 15% to 20% of population has at least one acute eruption during their lifetime, resulting in 1% to 2% of dermatological and allergological visits. Urticaria is classified based on its temporal evolution as acute (less than 6 weeks or chronic (more than 6 weeks. Management strategies may involve non-pharmacological measures and drug interventions, which are grouped into first- (antihistamines, second- (corticosteroids and anti-leukotrienes and third-line therapies (immunomodulators. Stronger, but potentially riskier, second- and third-line management may be justified for patients who do not respond to first-line therapy, or whenever a specific etiology cannot be determined, such as in autoimmune urticaria.

  18. Development of plasma 21-deoxycortisol radioimmunoassay and application to the diagnosis of patients with 21-hydroxylase deficiency

    Energy Technology Data Exchange (ETDEWEB)

    Milewicz, A.; Vecsei, P.; Korth-Schuetz, S.; Haack, D.; Lichtwald, K.; Lewicka, S. (Heidelberg Univ. (Germany, F.R.)); Roesler, A. (Hadassah Medical School, Jerusalem (Israel)); Mittelstaedt, G.V. (Heidelberg Univ. (Germany, F.R.). Dept of Nephrology, Medical Faculty)

    1984-08-01

    Specific 21-deoxycortisol (21-DF) antiserum was raised in rabbits using a 21-DF-3,20-oxime-bovine serum albumin complex. Plasma radioimmunoassay of 21-DF was developed and used together with a radioimmunoassay of 17-hydroxyprogesterone (17-OH-P) for diagnosis of patients with 21-hydroxylase deficiency of congenital and postpubertal forms. The response of plasma 21-DF and 17-OH-P to iv.v. ACTH (25 IU) was studied in 15 adult controls and compared to 8 women with the late onset form of 21-hydroxylase deficiency and 23 women with idiopathic hirsutism. Normal 21-DF values for women were 6.9 +- 3.6 ng/dl and for men 9.71 +- 2.73 ng/dl. Newborn children (age: 3-10 days) had a value of 8.3 +- 4.8 ng/dl. During the menstrual cycle the 21-DF values did not change. The baseline and post-stimulated concentrations of hormone were similar in controls and women with hirsutism but significantly higher in women with the late onset form of 21-hydroxylase deficiency. In the congenital form of 21-hydroxylase deficiency, 21-DF values (baseline) were high. In general, the 21-DF and 17-OH-P values have shown parallel changes. However, one case of 21-hydroxylase deficiency with elevated 21-DF but normal 17-OH-P was observed. The use of 21-DF for the diagnosis of 21-hydroxylase deficiency is suggested.

  19. Rapid, semi-automated, and inexpensive radioimmunoassay of cAMP: application in GPCR-mediated adenylate cyclase assays.

    Science.gov (United States)

    Brown, Justin T; Kant, Andrew; Mailman, Richard B

    2009-03-15

    Cyclic AMP (cAMP) is an important signal transduction second messenger that is commonly used as a functional mirror on the actions of G protein-coupled receptors that can activate or inhibit adenylate cyclases. A radioimmunoassay for cAMP with femtomole sensitivity was first reported by Steiner more than 30 years ago, and there have been several subsequent modifications that have improved this assay in various ways. Here we describe additional improvement to existing methods that markedly improve speed and reduce cost without sacrificing sensitivity, and is also adaptable to analysis of cGMP. The primary antibody is coupled directly to magnetic beads that are then separated from unbound marker using filtration on microplates. This eliminates the need for a secondary antibody, and markedly increases throughput. In addition, we report a simple, reproducible, and inexpensive method to make the radiomarker used for this assay. Although still requiring the use of radioactivity, the resulting method retains a high degree of accuracy and precision, and is suitable for low-cost high throughput screening. Use of aspects of this method can also improve throughput in other radioimmunoassays.

  20. Desenvolvimento de um radioimunoensaio de dexametasona no soro como subsídio para o diagnóstico da síndrome de Cushing Development of serum dexamethasone radioimmunoassay to corroborate Cushing's syndrome diagnosis

    Directory of Open Access Journals (Sweden)

    Lilian Fukusima Hayashi

    2011-10-01

    Full Text Available INTRODUÇÃO: O teste de supressão com 1 mg de dexametasona (TSDx é amplamente empregado no rastreamento da síndrome de Cushing (SC dada sua elevada acurácia diagnóstica. A SC é um distúrbio endocrinometabólico resultante do hipercortisolismo crônico com característica de ausência de supressão do cortisol no TSDx. OBJETIVO: Desenvolver um radioimunoensaio (RIE para a dosagem de dexametasona (Dx no soro para complementar o TSDx. MÉTODOS: Foram imunizados três coelhos com o conjugado dexametasona-21-hemissuccinato-BSA para escolha do melhor anticorpo e o RIE foi desenvolvido de acordo com as recomendações da RE 899/2003 da Agência Nacional de Vigilância Sanitária (ANVISA. Analisamos 96 voluntários, sendo 67 submetidos ao TSDx e 29 não, e 12 pacientes com SC, estudados na Universidade Federal de São Paulo (UNIFESP e na Santa Casa de Misericórdia de São Paulo. RESULTADOS: O anticorpo contra Dx selecionado mostrou boa especificidade, coeficiente de variação (CV% intra e interensaio INTRODUCTION: The 1 mg dexamethasone suppression test (DxST is widely used to screen Cushing's syndrome (CS due to its high diagnostic accuracy. CS is an endocrine-metabolic disorder caused by hypercorticism, which is characterized by the absence of cortisol suppression in DxST. OBJECTIVE: To develop a radioimmunoassay (RIA for the measurement of serum dexamethasone (Dx to complement DxST. METHODS: Three rabbits were inoculated with dexamethasone-21-hemisuccinate-BSA in order to choose the best antibody. Serum Dx RIA was performed according to RE 899/2003 (Agência Nacional de Vigilância Sanitária [ANVISA] regulations. Serum samples from 96 volunteers from Universidade Federal de São Paulo (UNIFESP and Santa Casa de Misericórdia de São Paulo were analyzed, 67 of which were submitted to DxST and 29 were not. There were 12 patients with CS. RESULTS: The Dx antibody chosen showed good specificity. Intra- and interassay CV were < 20% with 93

  1. Use of radioimmunoassay as a screen for antibiotics in confined animal feeding operations and confirmation by liquid chromatography/mass spectrometry

    Science.gov (United States)

    Meyer, M.T.; Bumgarner, J.E.; Varns, J.L.; Daughtridge, J.V.; Thurman, E.M.; Hostetler, K.A.

    2000-01-01

    Approximately one-half of the 50 000000 lb of antibiotics produced in the USA are used in agriculture. Because of the intensive use of antibiotics in the management of confined livestock operations, the potential exists for the transport of these compounds and their metabolites into our nation's water resources. A commercially available radioimmunoassay method, developed as a screen for tetracycline antibiotics in serum, urine, milk, and tissue, was adapted to analyze water samples at a detection level of approximately 1.0 ppb and a semiquantitative analytical range of 1-20 ppb. Liquid waste samples were obtained from 13 hog lagoons in three states and 52 surface- and ground-water samples were obtained primarily from areas associated with intensive swine and poultry production in seven states. These samples were screened for the tetracycline antibiotics by using the modified radioimmunoassay screening method. The radioimmunoassay tests yielded positive results for tetracycline antibiotics in samples from all 13 of the hog lagoons. Dilutions of 10-100-fold of the hog lagoon samples indicated that tetracycline antibiotic concentrations ranged from approximately 5 to several hundred parts per billion in liquid hog lagoon waste. Of the 52 surface- and ground-water samples collected all but two tested negative and these two samples contained tetracycline antibiotic concentrations less than 1 ppb. A new liquid chromatography/mass spectrometry method was used to confirm the radioimmunoassay results in 9 samples and also to identify the tetracycline antibiotics to which the radioimmunoassay test was responding. The new liquid chromatography/mass spectrometry method with online solid-phase extraction and a detection level of 0.5 ??g/l confirmed the presence of chlorotetracycline in the hog lagoon samples and in one of the surface-water samples. The concentrations calculated from the radioimmunoassay were a factor of 1-5 times less than those calculated by the liquid

  2. Radioimmunoassay of ricin A- and B-chains applied to samples of ricin A-chain prepared by chromatofocusing and by DEAE Bio-Gel A chromatography

    Energy Technology Data Exchange (ETDEWEB)

    Ramakrishnan, S.; Eagle, M.R.; Houston, L.L. (Kansas Univ., Lawrence (USA). Dept. of Biochemistry)

    1982-11-24

    A radioimmunoassay for ricin and ricin A- and B-chains was developed. Amounts as low as 100 pg of A-chain and 500 pg of B-chain could easily be quantitated. We showed, however, that the free chains were more reactive in the radioimmunoassay than the equivalent quantity of the individual chains when combined in intact ricin. The usefulness of the assay was demonstrated by determining the concentration of contaminating A- or B-chains in preparations of the separate polypeptides purified by DEAE Bio-Gel A chromatography and by chromatofocusing.

  3. The development of a radioimmunoassay for reverse triiodothyronine sulfate in human serum and amniotic fluid

    Energy Technology Data Exchange (ETDEWEB)

    Wu, Sing-Yung (Veterans Administration Medical Center, Long Beach, CA (United States)); Huang, Wen-Sheng; Chen, Wei-Lian (Tri-Service General Hospital, Taipei (Taiwan, Province of China)); Polk, D.; Reviczky, A.; Williams, J. III; Chopra, I.J.; Fisher, D.A. (Univ. of California, Los Angeles (United States))

    1993-06-01

    Sulfated iodothyronines including T[sub 4]-sulfate (T[sub 4]S) and T[sub 3]-sulfate (T[sub 3]S) have been identified in human serum and amniotic fluid. Little is know, however, about the existence of sulfate conjugation of reverse T[sub 3] (rT[sub 3]S) in man. In this report, the authors employed a novel, sensitive, and specific rT[sub 3]S RIA to address this question. The rabbit antiserum to rT[sub 3]S was highly specific; T[sub 4], T[sub 3], rT[sub 3], and 3,3'-T[sub 2] showed less than 0.002% cross-reaction with the antiserum. Only T[sub 4]S and T[sub 3]S cross-reacted significantly (0.3% and 0.01%, respectively); other analogs cross-reacted less than 0.0001%. The detection threshold of the RIA was 14 pmol/L (1.0 ng/dL). The mean serum rT[sub 3]S concentration (pmol/L) was 40 in euthyroid subjects. Values were similar in hypothyroid patients (38) and pregnant women (52) but significantly (P < 0.01) elevated to 176 in hyperthyroid patient, 74 in patients with nonthyroid illnesses, and 684 in cord sera of newborns. Serum rT[sub 3]S increased significantly in hyperthyroid patients 1 day after administration of 1 g sodium ipodate orally. Reverse T[sub 3]S was detected consistently in amniotic fluid at 14 to 22 weeks of gestation and showed a marked rise 1-3 weeks after intraamniotic administration of 500-1000 [mu]g T[sub 4]. The various data suggest that : (1) rT[sub 3]S is a normal component of human serum and amniotic fluid; (2) it is derived from metabolism of T[sub 4] or rT[sub 3]; (3) circulating rT[sub 3]S increases in hyperthyroidism and in circumstances where type I 5'-monodeiodinating activity is low, e.g. nonthyroid illnesses, fetal life, and after administration of ipodate. 20 refs., 4 figs.

  4. Radioimmunoassay for 3',5'-diiodothyronine. [/sup 125/I tracer technique

    Energy Technology Data Exchange (ETDEWEB)

    Burman, K.D.; Wright, F.D.; Smallridge, R.C.; Green, B.J.; Georges, L.P.; Wartofsky, L.

    1978-11-01

    The present report describes a RIA for 3',5'-diiodothyronine (T/sub 2/) that can be performed on unextracted serum and which has a lower limit of detectability of 2 ng/dl. Cross-reactivity with other iodothyronines was negligible, except for rT/sub 3/ which began to demonstrate cross-reactivity when rT/sub 3/ levels were elevated to 180 ng/dl. Employing this RIA for T/sub 2/, we have determined that 83 healthy individuals had a mean (+-SE) serum T/sub 2/ concentration of 5.0 +- 0.3 ng/dl, thyrotoxic subjects (n = 12) had a mean T/sub 2/ level that was elevated to 10.8 +- 0.8 ng/dl, and each of 6 hypothyroid subjects had undetectable (<2 ng/dl) concentrations. Athyreotic patients (n = 8), receiving 0.4 mg T/sub 4/ daily, had serum T/sub 2/ concentrations of 15.0 +- 3.0 ng/dl. Fasting in obese subjects was associated with an increase in serum T/sub 2/ to 6.9 +- 0.6 ng/dl from a basal level of 4.4 +- 0.4 ng/dl in the fed state (P < 0.01). Despite the fact that rT/sub 3/ levels may be elevated in amniotic fluid and that rT/sub 3/ is expected to represent the major source from which extrathyroidal T/sub 2/ arises, T/sub 2/ levels were low in amniotic fluid, being undetectable (<2 ng/dl) in 9 of 19 samples; the mean (+-SE) T/sub 2/ concentration in the 10 detectable samples was 5.4 +- 1 ng/dl. These data indicate T/sub 2/ is a normal component of serum and that the majority of serum T/sub 2/ is probably derived from peripheral conversion. Furthermore, these observations suggest that situations associated with elevated rT/sub 3/ levels (e.g., thyrotoxicosis and fasting) may also have increased T/sub 2/ values.

  5. Application of different /sup 125/I tracers in radioimmunoassays of estradiol-17. beta

    Energy Technology Data Exchange (ETDEWEB)

    Bienert, R.; Flentje, H.; Herzmann, H. (Akademie der Wissenschaften der DDR, Berlin-Buch. Zentralinstitut fuer Isotopen- und Strahlenforschung; Akademie der Wissenschaften der DDR, Leipzig. Zentralinstitut fuer Isotopen- und Strahlenforschung)

    1984-01-01

    Some different /sup 125/I-labelled estradiol tracers were produced by direct radioiodizing of estradiol and also of the histamine and tyramine conjugates of estradiol-3-carboxymethylether (E/sub 2/-3-CM) by means of the chloramine-T method. The linkage properties of these tracers were investigated in relation to the /sup 3/H-labelled estradiol opposite to the antisera, which were produced against the cow serum albumin (RSA) conjugates of E/sub 2/-3-CM and estradiol-6-carboxymethyloxime (E/sub 2/-6-CMO). As suitable system for the radioimmunological estradiol determination could be revealed 4-/sup 125/I-iodine estradiol in connection with one antiserum in each case of the radioligand antiserum combinations against E/sub 2/-3-CM-RSA- and E/sub 2/-6-CMO-RSA-conjugate. The double antibody method is used for separation in optimized RIA systems. The first and the second antibody reaction take place simultaneously.

  6. Improved radioimmunoassay for thymosin. cap alpha. 1 recognizes the N-14 amino terminus

    Energy Technology Data Exchange (ETDEWEB)

    Naylor, P.H.; Goldstein, A.L.

    1986-03-01

    Thymosin ..cap alpha../sub 1/(T..cap alpha../sub 1/) is a biologically active thymic peptide currently undergoing trials as an immunomodulator in cancer patients and patients with immunodeficiencies. Abnormally elevated levels of T..cap alpha../sub 1/ have been found in the serum of individuals with or at risk for AIDS, with T-cell leukemias, and chronic progressive multiple sclerosis. Absorption of the current antibody with a synthetic C-14 fragment of T..cap alpha../sub 1/ results in an antisera specific for the N-14 amino terminus of T..cap alpha../sub 1/, which measures significantly higher levels of T..cap alpha../sub 1/ in serum from normal individuals and significantly increases the sensitivity of the assay. Ongoing studies indicate that this new RIA for T..cap alpha../sub 1/ will be useful in monitoring changes of immunoreactive T..cap alpha../sub 1/ in serum with age and in patients with known or suspected T-cell abnormalities.

  7. Aprendizado e memória

    OpenAIRE

    Lombroso, Paul

    2004-01-01

    A memória é dividida de duas grandes formas: explícita e implícita. O hipocampo é necessário para a formação das memórias explícitas, ao passo que várias outras regiões do cérebro, incluindo o estriado, a amígdala e o nucleus accumbens, estão envolvidos na formação das memórias implícitas. A formação de todas as memórias requer alterações morfológicas nas sinapses: novas sinapses devem ser formadas ou antigas precisam ser fortalecidas. Considera-se que essas alterações reflitam a base celular...

  8. Development and standardization of radioimmunoassay technique for human proinsulin determining and its use in the study of type II diabetes mellitus associated to obesity; Desenvolvimento e padronizacao da tecnica de radioimunoensaio para a determinacao de pro-insulina humana e sua aplicacao no estudo do diabetes mellitus tipo II associado a obesidade

    Energy Technology Data Exchange (ETDEWEB)

    Nascimento, Martha do

    1996-12-31

    The availability of immunoassay methodology for proinsulin is important to define its physiological and pathophysiological significance in humans. Serum concentration of proinsulin are elevated in patients with type II Diabetes Mellitus (NIDDM) and recently diagnosed Type I, so a raised circulating concentration of proinsulin may serve as an early indicator of {beta} cells dysfunction. recently, in NIDDM the serum concentrations of proinsulin and its B-chain-C-peptide junctional split form, des (31-32), were found to correlate with diastolic blood pressure, a risk factor for cardiovascular disease. The development of a sensitive and specific radioimmunoassay (RIA) methodology for proinsulin has been difficult due to its low concentration in serum and the presence of proinsulin conversion intermediates in fluids and tissues. Also other potentially cross-reactive peptides, including insulin and C-peptide, can interfere in the assay. This work describe a highly specific human proinsulin RIA developed by using biosynthetic human proinsulin (hPI) as immunogen, standard and tracer. (author) 133 refs., 17 figs., 36 tabs.

  9. Purification of human hepatic glutathione S-transferases and the development of a radioimmunoassay for their measurement in plasma

    Energy Technology Data Exchange (ETDEWEB)

    Hayes, J.D.; Gilligan, D.; Beckett, G.J. (Edinburgh Univ. (UK). Dept. of Clinical Chemistry); Chapman, B.J. (Royal Infirmary, Edinburgh (UK))

    1983-10-31

    A purification scheme is described for six human hepatic glutathione S-transferases from a single liver. Five of the transferases comprised Ya monomers and had a molecular mass of 44000. The remaining enzyme comprised Yb monomers and had a molecular mass of 47000. Data are presented demonstrating that there are at least two distinct Ya monomers. A radioimmunoassay has been developed that has sufficient precision and sensitivity to allow direct measurement of glutathione S-transferase concentrations in unextracted plasma. A comparison of aminotransferase and glutathione S-transferase levels, in three patients who had taken a paracetamol overdose, indicated that glutathione S-transferase measurements provided a far more sensitive index of hepatocellular integrity than the more conventional aminotransferase measurements.

  10. 3',5'-diiodothyronine in health and disease: studies by a radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Chopra, I.J.; Geola, F.; Solomon, D.H.; Maciel, R.M.B.

    1978-12-01

    An RIA has been developed for 3'5'-diiiodothyronine (3',5'-T/sub 2/) in unextracted serum. Interference in binding of radioactive 3',5'-T/sub 2/ to anti-3',5'-T/sub 2/ was minimized by using phosphate buffer and merthiolate. The detection threshold of the RIA was 2.5 ng/dl. Recovery of nonradioactive 3',5'-T/sub 2/ averaged 99%. T/sub 4/, T/sub 3/, and rT/sub 3/ cross-reacted with anti-3',5'-T/sub 2/ antibody 0.0025, < 0.0004, and 0.22%, respectively. The 3'-monoiodothyronine cross-reacted 1.7%. Mean serum 3',5'-T/sub 2/ concentrations in ng/dl were 2.4 in 53 normal subjects, 4.2 in 7 hypothyroid patients, 14.9 in 34 hyperthyroid patients, 13.5 in 25 patients with hepatic cirrhosis, and 14.3 in 31 newborns' cord blood serum. The values for the latter four groups were significantly different from normal. The serum 3',5'-T/sub 2/ concentration of 7.7 ng/dl in eight subjects in the third trimester of pregnancy did not differ from normal when serum T/sub 4/ and T/sub 3/ were elevated. Oral administration of 300 ..mu..g rT/sub 3/ to 9 normal subjects led to an increase in serum 3'5'-T/sub 2/ concentration of 45% at 1h. Total fasting in 3 obese subjects was associated with an increase in serum 3',5'-T/sub 2/ from 8.6 to 16.3 ng/dl at 6 to 8 days; rT/sub 3/ increased similarly, while T/sub 3/ decreased and T/sub 4/ did not change. Administration of dexamethasone to 4 hyperthyroid patients was associated with nearly parallel increases in serum 3',5'-T/sub 2/ and rT/sub 3/ and a decrease in T/sub 3/. The 3',5'-T/sub 2/ concentrations in amniotic fluids were 15.2 ng/dl at 15 to 20 weeks gestation and 5.8 ng/dl at 33 to 40 weeks. Pronase hydrolysates of 9 normal thyroid glands contained 350 ..mu..gT/sub 4/ and 0.24 ..mu..g 3',5'-T/sub 2//g wet wt. It was estimated that thyroidal secretion contributes < 1% of 3',5'-T/sub 2/ in

  11. Immunoassay detection of drugs in racing horses. IX. Detection of detomidine in equine blood and urine by radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Wood, T.; Tai, C.L.; Taylor, D.G.; Woods, W.E.; Wang, C.J.; Houtz, P.K.; Tai, H.H.; Weckman, T.J.; Yang, J.M.; Sturma, L.

    1989-02-01

    Detomidine is a potent non-narcotic sedative agent which is currently in the process of being approved for veterinary clinical use in the United States. Since no effective screening method in horses is available for detomidine, we have developed an /sup 125/I radioimmunoassay for detomidine in equine blood and urine as part of a panel of tests for illegal drugs in performance horses. Our /sup 125/I radioimmunoassay has an I-50 for detomidine of approximately 2 ng/ml. Our assay shows limited cross-reactivity with the pharmacodynamically similar xylazine, but does not cross-react with acepromazine, epinephrine, haloperidol or promazine. The plasma kinetic data from clinical (greater than or equal to 5 mg/horse) as well as sub-clinical doses indicate first-order elimination in a dose-dependent manner. Within the first 30 minutes after intravenous (IV) administration of 30 mg/horse, plasma levels peak at approximately 20 ng/ml and then decline with an apparent plasma half-life of 25 minutes. Diuresis can occur with administration of clinical doses of detomidine and this effect was accounted for in the analysis of urine samples. Using this method, administration of 30 mg/horse can be readily detected in equine urine for up to 8 hours after IV injection. Additionally, doses as low as 0.5 mg/horse can be detected for short periods of time in blood and urine with use of this assay. Utilization of this assay by research scientists and forensic analysts will allow for the establishment of proper guidelines and controls regarding detomidine administration to performance horses and assurance of compliance with these guidelines.

  12. Antibody response to the lipopolysaccharide and protein antigens of Salmonella typhi during typhoid infection. I. Measurement of serum antibodies by radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Tsang, R.S.W.; Chau, P.Y.; Lam, S.K. (Hong Kong Univ.); La Brooy, J.T.; Rowley, D. (Adelaide Univ. (Australia))

    1981-12-01

    Serum antibody responses to the lipopolysaccharide and protein antigens of S. typhi in typhoid patients were studied using a solid-phase radioimmunoassay technique with /sup 125/I labelled anti-immunoglobulin antibody. Sera from 24 adult typhoid patients and 20 non-typhoid adult controls were compared. As a group, sera from typhoid patients showed increased IgA, IgG and IgM immunoglobulin levels and gave significantly higher anti-LPS and anti-protein antibody titres in all three major immunoglobulin classes than did non-typhoid controls. Levels of antibodies against LPS or protein in sera of typhoid patients were highly variable with a skew distribution. A good correlation was found between antibody titres to the LPS antigen and those to a protein antigen. No correlation, however, was found between the anti-LPS antibody titres measured by radioimmunoassay and the anti-O antibody titres measured by the Widal agglutination test. Titration of anti-LPS or anti-protein antibodies by radioimmunoassay was found to be more sensitive and specific than Widal test for the serological diagnosis of typhoid fever. The advantages of measuring antibody response by radioimmunoassay over conventional Widal test are discussed.

  13. Operation of pneumococcal polysaccharide radioimmunoassay reference laboratory: coordination of the serological aspects of otitis media field trials. Annual report 28 Jun 77--27 Jun 78

    Energy Technology Data Exchange (ETDEWEB)

    Schiffman, G.

    1978-04-03

    The contract supports a serologic reference laboratory for the performance of radioimmunoassay of antibodies to pneumococcal polysaccharides. Antibody assays have been performed for a number of investigators studying the response of humans to pneumococcal vaccines. In addition, a large quantity of labeled polysaccharides for use in the assay have been prepared and stored.

  14. 2003 RIA R AND D WORKSHOP.

    Energy Technology Data Exchange (ETDEWEB)

    OZAKI, S.ET AL.

    2003-08-26

    The 2003 RIA R&D Workshop was held on August 26-28, 2003 at the Four Points Sheraton Hotel in Bethesda, Maryland. This Workshop was chaired by Satoshi Ozaki of BNL and sponsored by the Nuclear Physics Division of DOE, with the help of Oak Ridge Institute for Science and Education (ORISE). The purpose of this workshop was to understand the present status of R&D efforts for RIA, to evaluate the needs for further R&D, and to identify opportunities for international collaborations. The workshop examined and documented the current pre-conceptual design for RIA, identifying areas where decisions on technical options remain. The status of the current RIA R&D program was documented, recognizing areas where efforts were needed in light of what had been learned. The ongoing and planned R&D activities for operating and planned rare-isotope facilities were presented, enabling the workshop to be a venue to develop coordinated R&D efforts of mutual benefit to U.S. and international efforts. The scientific program for the first day (August 26, 2003) consisted mostly of invited talks presented by major research groups involved in RIA and other RI beam facilities. The talks included those covering: Science of RIA and the RIA Facility Performance Requirements; The Reference RIA Facility Pre-CDR design that was used for the NSAC cost exercise (M. Harrison Sub-Panel) in January 2001; New or latest perspectives on the RIA design at ANL & MSU; and RI Beam facility plans and overview of the R&D activities at overseas laboratories. The second day (August 27, 2003) was devoted to contributed talks on continuing R&D, including that which had been supported by DOE RIA R&D funds. The third day (August 28, 2003) began with open panel discussions in the morning, including further input from participants. The panel members discussed the present status of the RIA planning and R&D needs in a closed session for the rest of the day, and then worked on report planning and writing. This Workshop

  15. Leituras de operárias

    Directory of Open Access Journals (Sweden)

    Betty Mindlin

    2008-03-01

    Full Text Available Defensora de causas sociais, paladina poética contra injustiças e desigualdade, Ecléa Bosi em seu livro Cultura Popular e Cultura de Massa: Leituras de Operárias, pesquisa as leituras de cerca de 50 operárias, procurando ver que acesso têm ao imaginário, aos livros, quais as suas condições de vida, como a sociedade industrial as privam da criação artística e literária, apesar de sua sede de conhecimento e de expressão.

  16. Analysis Atrazine Residues in Water Using Radioimmunoassay%用放射免疫测定法分析水中莠去津残留量的研究

    Institute of Scientific and Technical Information of China (English)

    徐德武; 朱永清; 杨根海; 楚慧民; 阎美玉

    2000-01-01

    The determination of atrazine residue in water with radioimmunoassay method was studied. The High bioactive antigen was synthesized and the high titer anti-atrazine antibody was prepared. The antiserum was specific for atrazine, the cross-reactivity of the antiserum to propazine and simazine was 93 % and 8 %, respectively. The RIA for atrazine labeled by 3H was established and showed that the rate of recovery by adding atrazine in drink water and yunhe water were 88.5% -107.5 % and 87.4 %-110.9 % respectively.%以莠去津均三氮苯类结构特征,合成连有活性羧基的莠去津半抗原,通过活性酯法制备出具有生物活性的人工抗原,以此免疫兔子获得抗莠去津的多克隆抗体。抗体的特异性强,与扑灭津的交叉反应性为93%,与西玛津的交叉反应性为8%。以3H标记莠去津建立的莠去津放射免疫测定法对水中莠去津添加回收率的测定表明,自来水的添加回收率为88.5%~107.5%,运河水的添加回收率为87.4%~110.9%。

  17. Anastomose mamária-coronária: análise de 2923 casos

    OpenAIRE

    1987-01-01

    Foi a partir de 1972 que, no Instituto Dante Pazzanese de Cardiologia, se iniciou o emprego da anastomose mamária-coronária associada, ou não, a pontes de safena, ou a outros procedimentos. Nessa época, apenas a artéria mamária interna esquerda (AMIE) foi usada (57 casos) para a revascularização da descendente anterior (DA) ou diagonal. No período 1973/1974 (386 casos), já ocorreram as primeiras anastomoses seqüenciais AMIE-DA/DA, assim como a artéria mamária interna direita (AMID) passou a s...

  18. INTERFACE ENTRE MEMÓRIA E LEITURA

    Directory of Open Access Journals (Sweden)

    Pedro Nunes de Castro

    2008-04-01

    Full Text Available Leitura e memória são realidades que se coadunam, na medida em que são interdependentes. Esse artigo nasce a partir de uma afirmação de Ivan Izquierdo, neurologista e especialista em memória. Ele assevera que a leitura é a forma mais completa de exercitar a memória. Busco subsídios, essencialmente na Psicologia Cognitiva, para entender essa categórica afirmação. Relaciono alguns aspectos sobre o processamento de textos, e classifico as memórias conforme a função, conteúdo e tempo de duração. A partir disso, delineio a interface comum e intrínseca entre essas duas realidades.

  19. NARRATIVAS SOBRE A TRAJETÓRIA MIGRATÓRIA: O RETORNO DOS BRASIGUAIOS

    OpenAIRE

    Silvia Lima de Aquino

    2017-01-01

    Neste artigo realizamos uma análise da trajetória migratória de pequenos agricultores brasileiros em direção ao Paraguai e seu retorno ao Brasil, a partir do ingresso em acampamentos organizados por agricultores sem-terra. Nesta análise, consideraremos as percepções dos próprios migrantes a respeito de suas trajetórias migratórias e, em especial, sobre o retorno ao Brasil. Assim, nos interessou examinar os fatores que propiciaram a migração desses indivíduos ou de seus antepassados ao campo n...

  20. Immunodiagnosis of systemic aspergillosis. I. Antigenemia detected by radioimmunoassay in experimental infection. [/sup 125/I tracer technique

    Energy Technology Data Exchange (ETDEWEB)

    Weiner, M.H.; Coats-Stephen, M.

    1979-01-01

    Because systemic aspergillosis is difficult to diagnose ante mortem, a study to improve immunodiagnosis was undertaken in a rabbit model of disseminated infection. We found that the predominant humoral response of infected animals was directed against four Aspergillus antigens identified by crossed immunoelectrophoresis. One of these antigens, a cell-wall carbohydrate, was purified by gel-filtration chromatography and was used to develop a radiommunoassay. The sensitivity of this assay was increased by testing for serum-bound antigen as well as for free antigen. When the sensitivity of the RIA was evaluated in the animal model, antigenemia was detected in 78% of 51 rabbits with disseminated infection and ante mortem in 86% of 42 rabbits with lethal infection. By contrast, with immunoprecipitin analysis only eight of 51 rabbits were positive for antigen, and six of 51 rabbits were positive for Aspergillus antibody. The specificity of the RIA was also tested. Negative controls for antigen included sera from 76 normal rabbits and sera from 25 rabbits with systemic candidiasis. The Candida control group is pertinent because 48% of these rabbits had specific Candida antigenemia detected by a mannan RIA. This study demonstrates that Aspergillus antigenemia occurs during the course of experimental disseminated aspergillosis and illustrates the potential of an Aspergillus antigen RIA for sensitive, specific immunodiagnosis of human infections.

  1. Radioimmunoassay of a human serum growth factor for Balb/c-3T3 cells: derivation from platelets.

    Science.gov (United States)

    Antoniades, H N; Scher, C D

    1977-05-01

    A radioimmunoassay has been developed for the detection and quantification of a human serum polypeptide that has growth-promoting activity for confluent Balb/c-3T3 cells. Antiserum to this growth factor does not recognize antigens in mouse, guinea pig, or bovine serum but does detect some crossreacting antigen in the serum of the New World monkey Cebus albifrons and more in the serum of the Old World rhesus monkeys Macaca mulatta and M. fascicularis, demonstrating that the antigenic determinants of the growth factor have a degree of species specificity. Serum derived from whole human blood contains approximately 770 pg of the growth factor per mg of protein; serum derived from platelet-poor blood contains about 112 pg of the growth factor per mg of protein. As much as 1 microng of the growth factor per mg of protein has been recovered from human platelets by heating them at 100 degrees for 2 min. Approximately 1-2 ng of the growth factor, in either whole serum or platelets, stimulates 5 to 10 X 10(3) confluent Balb/c-3T3 cells to replicate. The heat treatment of platelets allows the purification and quantitative recovery of the growth factor from blood.

  2. Serum prostate-specific acid phosphatase: development and validation of a specific radioimmunoassay. [/sup 125/I tracer technique

    Energy Technology Data Exchange (ETDEWEB)

    Vihko, P.; Sajanti, E.; Jaenne, O.; Peltonen, L.; Vihko, R.

    1978-11-01

    We describe radioimmunoassay for human prostatic acid phosphatase (orthophosphoric-monoester phosphohydrolase (acid optimum), EC 3.1.3.2) in serum, with use of monospecific antisera raised in rabbits against highly purified acid phosphatase from human prostates. The antiserum did not cross react with partly purified acid phosphatases from human spleen, erythrocytes, or synovial tissues. /sup 125/I-labeled acid phosphatase was prepared by a Chloramine T method, and the bound and free antigen was separated in the assay by use of anti-rabbit gamma-globulin raised in sheep. Uniform low nonspecific binding of the (/sup 125/I)acid phosphatase was achieved by using acid-phosphatase-free serum to prepare standard curves and diluted samples of serum with high acid phosphatase activities. Concentrations of immunoreactive acid phosphatase in the serum of healthy men ranged from <1 to 10 ..mu..g/liter and for 12 patients with advanced prostatic carcinoma between 100 and 500 ..mu..g/liter. The concentrations of the enzyme in sera of patients with benign prostatic hyperplasia were very similar to those in sera of the reference group.

  3. An M-antibody capture radioimmunoassay (MACRIA) for detection of JC virus-specific IgM.

    Science.gov (United States)

    Knowles, W A; Gibson, P E; Hand, J F; Brown, D W

    1992-10-01

    A solid-phase M-antibody capture radioimmunoassay (MACRIA) for detecting JC-specific IgM is described. The assay is based on a JC-specific monoclonal antibody (17.7.6) and Nonidet P40-treated, glycine-extracted antigen. MACRIA is more sensitive for JC IgM detection than haemagglutination inhibition (HI) following serum fractionation on a sucrose density gradient, and can be applied to large numbers of sera. The specificity of the assay was confirmed by examining sera from several acute virus infections and also those containing rheumatoid factor. Sera collected from renal transplant recipients with known active JC virus infection were found to contain more than 5 units of JC IgM. In this group of patients JC IgM represents either primary or reactivated JC infection. JC IgM was detected by MACRIA in 15 of 100 unselected blood donors, indicating that JC IgM is frequently produced in healthy seropositive individuals. Thirteen of the 15 sera positive from blood donors contained only low levels of JC IgM (< 5 units), but the specificity of all these results was confirmed in a blocking assay. It is suggested that these low levels of JC IgM may occur in up to 28% of seropositive individuals and result from active JC antigenic stimulation in healthy immunocompetent adults.

  4. Radioimmunoassay for 3,3'-L-diiodothyronine (3,3'T/sub 2/)

    Energy Technology Data Exchange (ETDEWEB)

    Burman, K.D.; Strum, D.; Dimond, R.C.; Djuh, Y.Y.; Wright, F.D.; Earll, J.M.; Wartofsky, L.

    1977-08-01

    The report describes the development of a radioimmunoassay for 3,3'-L-diiodothyronine (3,3'T/sub 2/) which may be performed on unextracted serum. Utilizing a specific antiserum to 3,3'-L-T/sub 2/-bovine serum albumin conjugates developed in rabbits, cross-reactivity was less than 0.5% with 3,5,3'-triiodothyronine (T/sub 3/) and 3,3',5'-triiodothyronine (reverse T/sub 3/) and less than 0.01% with thyroxine (T/sub 4/). Intra-assay variation averaged 2.9% and inter-assay variation was 7.8% and 18.5% when serum samples with 3,3'T/sub 2/ concentrations of 8 ng/dl and 12 ng/dl, respectively, were analyzed. Assay sensitivity was considered to be 6 ng/dl by statistical criteria. The data suggest that 3,3'T/sub 2/ circulates in the serum of normal individuals and tends to parallel serum concentrations of T/sub 3/, T/sub 4/ and rT/sub 3/ in various states of thyroid function.

  5. A sup 125 I-radioimmunoassay for measuring androstenedione in serum and in blood-spot samples from neonates

    Energy Technology Data Exchange (ETDEWEB)

    Thomson, S.; Wallace, A.M.; Cook, B. (Stobhill Hospital, Glasgow (England))

    1989-08-01

    We developed a radioimmunoassay with a gamma-emitting radioligand to measure androstenedione in human serum and in dried blood-spot samples from newborns. Antisera were raised in rabbits against androstenedione linked to bovine serum albumin at positions 3, 6, or 11 on the steroid nucleus. Radioligands were prepared by linking ({sup 125}I)iodohistamine at positions 3, 6, or 11. Linkages were through either carboxymethyloxime or hemisuccinate bridges. All label and antibody combinations were examined, and the most sensitive and specific combination (antiserum raised against androstenedione-3-carboxymethyloxime-bovine serum albumin with an androstenedione-carboxymethyloxime-({sup 125}I)iodohistamine label) was selected for full evaluation. We report the performance of these selected reagents in an immunoassay for androstenedione in both serum and dried blood-spot samples from neonates. We measured concentrations of androstenedione in serum under normal and pathological conditions such as congenital adrenal hyperplasia and polycystic ovarian disease. Diurnal variation in normal men was observed. Androstenedione was measured in blood spots from neonates born at term or prematurely, with respiratory distress syndrome, or with congenital adrenal hyperplasia.

  6. Elevation of plasma gonadotropin concentration in response to mammalian gonadotropin releasing hormone (GRH) treatment of the male brown trout as determined by radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Grim, L.W.; Cluett, D.M.

    1974-01-01

    Rapid increase of the plasma gonadotropin concentration as measured by radioimmunoassay has been demonstrated in response to GRH treatment of the sexually mature male brown trout. Peak gonadotropin values were observed within 15 minutes of GRH treatment, however, the return to baseline values was prolonged compared with the mammalian response. These data support the concept that the brain, operating via releasing hormones, plays a role in the control of pituitary hormone secretion in fish.

  7. Development and applications of a radioimmunoassay to human plasma kallikrein; Desenvolvimento e aplicacoes de um radioimunoensaio para a calicreina plasmatica humana

    Energy Technology Data Exchange (ETDEWEB)

    Marques, Roselane

    1986-12-31

    A radioimmunoassay to human plasma kallikrein (Hu PK) was developed using specific antibodies against the purified activates enzyme. The antiserum showed identity in the precipitation, double immuno-diffusion, immobility, immuno-electrophoresis, using as normal plasma as purified kallikrein. The protein {sup 125} I-kallikrein was prepared by chloramine-t method and presented homogeneity, stability, immunoactivity and high specific activity, so that a sensitive assay was assured. 193 refs., 19 figs., 4 tabs.

  8. Analytical accuracy of determinations of aminoglycoside concentrations by enzyme multiplied immunoassay, fluorescence polarization immunoassay, and radioimmunoassay in the presence of heparin.

    OpenAIRE

    O?Connell, M. E.; Heim, K L; Halstenson, C E; Matzke, G R

    1984-01-01

    The accuracy of gentamicin, netilmicin, and tobramycin concentration determinations by enzyme multiplied immunoassay technique (EMIT; Syva Corp., Palo Alto, Calif.), fluorescence polarization immunoassay (TDx; Abbott Diagnostics, Irving, Tex.), and radioimmunoassay were compared in the presence of 0 to 3,000 USP units of porcine heparin per ml. Gentamicin, netilmicin, and tobramycin concentrations determined by EMIT decreased by 10 and 50% in the presence of 75 and 1,000 USP units/ml, 2 and 5...

  9. Standardization of the method for measurement of plasma estrone by radioimmunoassay; Padronizacao do metodo de dosagem de estrona plasmatica por radioimunoensaio

    Energy Technology Data Exchange (ETDEWEB)

    Vilanova, M.S.V.; Moreira, A.C.; Sala, M.M. de; Sa, M.F.S. de [Sao Paulo Univ., Ribeirao Preto, SP (Brazil). Faculdade de Medicina

    1994-10-01

    The present paper has as objective standardize a radioimmunoassay method for measurement of plasma estrone. Ethyl ether was used for plasma extraction. The sensitivity (Minimal detectable dose) was 3,7 pg/tube; the reproducibility (inter assay error) was 8,6%; the precision (intra assay error) was 4,1%. As a biological control the plasma estrone was ml and in 24 patients with polycystic ovarian syndrome (median = 77,9 pg/ml). (author). 6 refs, 2 figs, 2 tabs.

  10. A colangiografia peroperatória

    Directory of Open Access Journals (Sweden)

    Eduardo Lemos de Souza Bastos

    Full Text Available Os autores revisam 96 procedimentos colangiográficos peroperatórios realizados no Departamento de Cirurgia da Faculdade de Medicina de Marília (FAMEMA entre janeiro de 1993 e fevereiro de 1995. Dos exames estudados, setenta foram considerados normais, 23 alterados e três inconclusivos. Os autores apresentam casos onde a colangiografia peroperatória (CPO não confirmou a suspeita pré-operatória, o que evitou explorações desnecessárias da via biliar (6,25% ou não permitiu diagnóstico incorreto (9,36%. Do exposto, os autores concluem que a CPO continuará sendo rotina nas operações sobre a vesícula e vias biliares e sugerem a necessidade do estabelecimento de padrão técnico para a realização do exame.

  11. A memória de Borges

    Directory of Open Access Journals (Sweden)

    Eneida Maria Souza

    2010-08-01

    nefasta ou de outra ordem? Sem atribuir algum juízo de valor ao debate, pretende-se apontar um dos pontos da poética borgiana responsável por sua consagração mundial, qual seja, o da autonomia literária. A presença/ausência da imagem do escritor/autor transforma-se em tema literário, intriga que se enreda/desenreda como espelho reduplicador da vida literária e da literatura.

  12. Heterologous radioimmunoassay for llama and alpaca luteinizing hormone with a monoclonal antibody, and equine standard and a human tracer

    Energy Technology Data Exchange (ETDEWEB)

    Aba, M.A.; Forsberg, M. [Swedish Univ. of Agricultural Sciences, Dept. of Clinical Chemistry, Faculty of Veterinary medicine, Uppsala (Sweden)

    1995-12-31

    A radioimmunoassay for llama and alpaca LH was developed using a human I{sup 125}LH tracer from a commercial kit, equine LH diluted in human LH free serum as standard, and amonoclonal antibody (518B7) specific for LH but with low species specificity. A 60-min delay in the addition of the tracer and overnight incubation gave a sensitivity of 0.8 {mu}3g L{sup -1}. The intra-assay coefficient of variation was 37% at 1 {mu}g L{sup -1}, declined to 15% at 4 {mu}g L{sup -1} and was below 6% for concentrations up to 32 {mu}g L{sup -1}. The inter-assay coefficients of variation for 3 control samples were 20% (2.8 {mu}g L{sup -1}), 16% (7.1 {mu}g L{sup -1}) and 9.8% (19 {mu}g L{sup -1}). In an attempt to increase sensitivity, all tubes were preincubated for 4 h at room temperature before adding the tracer, and the sample volume was increased from 50 {mu}L to 100 {mu}L (in the standard curve the increased volume was compensated for by human LH free serum). With this protocol, the assay sensitivity was 0,5 {mu}g L{sup -1}. The assay was validated clinically and demonstrated increased concentrations of LH after mating in llamas and alpacas. Furthermore, the assay was used to monitor LH responses to a single dose of GnRH in llamas (adult males and females at different ages). (au) 9 refs.

  13. Retrospective evaluation of the Du Pont radioimmunoassay kit for detection of Legionella pneumophila serogroup 1 antigenuria in humans.

    Science.gov (United States)

    Aguero-Rosenfeld, M E; Edelstein, P H

    1988-09-01

    We used the Du Pont radioimmunoassay kit for soluble Legionella pneumophila serogroup 1 antigenuria (Du Pont Co., Wilmington, Del.) to test 422 urine samples from patients with and without Legionnaires disease (LD). The urine specimens were collected from 23 patients with culture-proven LD and from 346 patients without LD. L. pneumophila serogroup 1 was isolated from 14 patients with culture-proven LD, and other L. pneumophila serogroups or other Legionella species were isolated from 9 patients; 58 urine specimens were tested from these 23 patients. The non-LD group was composed of 75 bacteremic patients (35 gram-negative and 40 gram-positive bacteremias), 7 patients with candidemia, 48 patients with non-LD pneumonia, 90 patients with gram-negative bacteriuria (greater than 10(5) CFU/ml), 23 patients with gram-positive bacteriuria (greater than 10(5) CFU/ml), 14 patients with candiduria (greater than 10(5) CFU/ml), and 89 outpatients with negative urine cultures. All tests were performed in duplicate, including positive and negative controls. Sample results with values greater than or equal to 3.0 times those of the negative controls were considered positive for L. pneumophila serogroup 1 antigenuria. The average sample-to-negative ratios were 19.1 for the L. pneumophila serogroup 1 specimens, and 1.0 for both the non-serogroup 1 legionella group and the non-LD specimens. All but one of the patients who were culture positive for L. pneumophila serogroup 1 had at least one specimen positive for serogroup 1 antigenuria; none of the non-L. pneumophila serogroup 1 patients had a positive urine test. The test was highly specific (100%) and sensitive (93%) for the detection of L. pneumophila serogroup 1 antigenuria. Concentrations of urine by vacuum evaporation increased test sensitivity without apparently affecting specificity.

  14. Evaluation of platelet thromboxane radioimmunoassay method to measure platelet life-span: Comparison with /sup 111/indium-platelet method

    Energy Technology Data Exchange (ETDEWEB)

    Vallabhajosula, S.; Machac, J.; Badimon, L.; Lipszyc, H.; Goldsmith, S.J.; Fuster, V.

    1985-05-01

    The platelet activation during radiolabeling in vitro with Cr-51 and In-111 may affect the platelet life-span (PLS) in vivo. A new RIA method to measure PLS is being evaluated. Aspirin inhibits platelet thromboxane (TxA/sub 2/) by acetylating cyclooxygenase. The time required for the TxA/sub 2/ levels to return towards control values depends on the rate of new platelets entering circulation and is a measure of PLS. A single dose of aspirin (150mg) was given to 5 normal human subjects. Blood samples were collected for 2 days before aspirin and daily for 10 days. TxA/sub 2/ production in response to endogenous thrombin was studied by allowing 1 ml blood sample to clot at 37/sup 0/C for 90 min. Serum TxB/sub 2/ (stable breakdown product of Tx-A/sub 2/) levels determined by RIA technique. The plot of TxB/sub 2/ levels (% control) against time showed a gradual increase. The PLS calculated by linear regression analysis assuming a 2-day lag period before cyclooxygenase recovery is 9.7 +- 2.37. In the same 5 subjects, platelets from a 50ml blood sample were labeled with /sup 111/In-tropolone in 2 ml autologous plasma. Starting at 1 hr after injection of labeled platelets, 10 blood samples were obtained over a 8 day period. The PLS calculated based on a linear regression analysis is 10.2 +. 1.4. The PLS measured from the rate of platelet disappearance from circulation and the rate of platelet regeneration into circulation are quite comparable in normal subjects. TxA/sub 2/ regeneration RIA may provide a method to measure PLS without administering radioactivity to patient.

  15. HISTÓRIAS DE RETIRANTES: RUÍNAS LITERÁRIAS NO CINEMA

    Directory of Open Access Journals (Sweden)

    Elisabete Alfeld Rodrigues

    2009-06-01

    Full Text Available Em O caminho das nuvens, Vicente Amorim conta a história da retirada de uma família do interior nordestino para a região sudeste. Uma história que já foi muitas vezes contada, por exemplo, em Vidas Secas: primeiro, na literatura, por Graciliano Ramos e, depois, no cinema, por Nélson Pereira dos Santos. Na retirada contada por Amorim, a migração não ocorre pelos passos na aridez do sertão; ela acontece por bicicletas nas rodovias, estradas e pequenas cidades que a família – pai, mãe e cinco filhos – percorre para chegar à cidade do Rio de Janeiro. A família se desloca da Paraíba em busca de um emprego de mil reais por mês que só pode ser encontrado no sudeste do Brasil – este motivo desencadeador parte de uma história factual. O filme é uma releitura que resgata a literatura, o cinema e a história factual promovendo uma relação dialógica construída por fragmentos das várias histórias.

  16. Development and validation of a radioimmunoassay for fish insulin-like growth factor I (IGF-I) and the effect of aquaculture related stressors on circulating IGF-I levels.

    Science.gov (United States)

    Dyer, Anthony R; Upton, Zee; Stone, David; Thomas, Philip M; Soole, Kathleen L; Higgs, Naomi; Quinn, Kirsty; Carragher, John F

    2004-02-01

    This paper describes the development and validation of a commercially available radioimmunoassay (RIA) for the detection of fish insulin-like growth factor-I (IGF-I). The assay was developed using recombinant barramundi IGF-I as antigen and recombinant tuna IGF-I as radiolabelled tracer and standard. Assay sensitivity was 0.15 ng/ml, inter-assay variation was 16% (n = 9) and intra-assay variation was 3% (n = 10). Cross reactivity of less than 0.01% was found with salmon insulin, salmon IGF-II and barramundi IGF-II, less than 0.5% with human IGF-I and less than 1% with human IGF-II. Parallel dose-response inhibition curves were shown for barramundi (Lates calcarifer), coho salmon (Oncorhynchus kisutch), Southern Bluefin tuna (Thunnus maccoyii), tilapia (Oreochromis mossambicus), and seabream (Pagrus auratus) IGF-I. The assay was then used to measure stress related changes in different aquacultured fish species. Salt water acclimated Atlantic salmon smolts (Salmo salar) bathed for 2 h in fresh water showed significantly lower IGF-I concentrations than control smolts two days after the bath (53.1 compared to 32.1 ng/ml), with levels of IGF-I also lower in smolts exhibiting stunted growth (stunts). Capture and confinement of wild tuna in sea-cages resulted in a significant decrease in IGF-I levels (28 ng/ml) when compared to tuna captured and sampled immediately (48 ng/ml), but had recovered to starting levels after 3 weeks (43 ng/ml). Handling and isolation in silver perch (Bidyanus bidyanus) led to a gradual decline in IGF-I over a 12 h period (36-19 ng/ml) but showed signs of recovery by 24 h (24 ng/ml) and had recovered fully 72 h after treatment (40 ng/ml). A similar trial in black bream (Acanthopagrus butcherii) showed comparable results with IGF-I levels gradually decreasing (40-26 ng/ml) over 24 h, results that were mirrored by cortisol concentrations which increased during this time (1-26 ng/ml). In the studies presented here changes in IGF-I levels were not

  17. Studies on the Radioimmunoassay of Human Growth Hormone - 2. The plasma HGH concentrations in the various febrile diseases

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Young Woo; Lee, Hong Kyu; Koh, Chang Soon; Lee, Mun Ho [Seoul National University College of Medicine, Seoul (Korea, Republic of)

    1972-03-15

    The plasma HGH concentrations were assayed in total 138 cases by the radioimmunoassay. The groups of control, typhoid fever, epidemic hemorrhagic fever, tuberculous meningitis and other febrile diseases were studied, also were the groups of hyperthyroidism, acromegaly and hypopituitarism. Insulin stimulation test was performed in control, typhoid fever and hypopituitarism. In the control group, the plasma HGH concentration in fasting (early morning) was 2.06+-1.183 m{mu}g/ml and its upper limit was 4.5 m{mu}g/ml. No sexual difference was observed. By the insulin stimulation, plasma HGH concentration had rised to the peak level of 24.1+-15.71 m{mu}g/ml, 60 min. after the intravenous insulin injection, then decreased to the normal level progressively. In typhoid fever, fasting HGH concentrations in febrile state and in defeverence were 2.5+-1.35 m{mu}g/ml and 2.2+-3.32 m{mu}g/ml respectively, showing no significant difference with the control group. However, the levels of individual cases ranged widely, compared with the control group. The response to the insulin stimulation test was similar to the control group. In epidemic hemorrhagic fever. the HGH concentrations in oliguric phase, in diuretic phase and in convalescence were 4.2+-3.71 m{mu}g/ml, 2.2+-l.30 m{mu}g/ml and 3.4+-3.01 m{mu}g/ml respectively. No significant differences were observe when compared to the control, but they showed wide range of plasma HGH levels. In tuberculous meningitis, the fasting HGH concentration was 2.9+-51.42 m{mu}g/ml. In the other febrile diseases, the value was 2.5+-2.23 m{mu}g/ml. In 4 cases of hypopituitarism, the fasting HGH concentration was 2.3+-0.42 m{mu}g/ml and ranged normally. However, the response to the insulin stimulation test was not observed. Very high plasma HGH concentrations were observed in acromegalic patients.

  18. Radioimmunoassay detection of levels of triiodothyronine and thyroxine in Mangalarga Marchador equine; Niveis sericos de triiodotinonina e tiroxina, atraves de radioimunoensaio, em equinos Mangalarga Marchador

    Energy Technology Data Exchange (ETDEWEB)

    Viana, F.A.B.; Pessoa, J.M.; Biondini, J. [Minas Gerais Univ., Belo Horizonte, MG (Brazil). Escola de Veterinaria

    1991-12-31

    Serum levels of triiodothyronine (T{sub 3}) and thyroxine (T{sub 4}) were determined in equine of Mangalarga Marchador breed through radioimmunoassay. Forty-two animals (17 males and 25 females), with age ranging from two to eighteen years, were utilized. The values recorded for males and females were, respectively: 101.68 {+-} 23.44 and 71.14 {+-} 18.82 ng/d l of T{sub 3} (P<0.05); 1.89 {+-} 0.58 and 2.94 {+-} 0.87 mc g/d l of T{sub 4} (P<0.05). (author). 10 refs, 1 tab.

  19. Purification of rabbit IgG, obtention of sheep anti rabbit IgG and their use in radioimmunoassay of avian leukosis virus p15

    Energy Technology Data Exchange (ETDEWEB)

    Higuchi, Tomoko; Ogata, Hiroe; Veiga, Silvio Sanches; Nogueira, Zelia Maria [Instituto de Quimica, Sao Paulo, SP (Brazil); Souza Felippe, Julia Maria Martins de [Instituto Adolfo Lutz, Sao Paulo, SP (Brazil). Div. de Virus

    1988-01-01

    Immunoglobulin from rabbit serum was purified by ammonium sulphate precipitation followed by chromatography on DEA-cellulose and gel filtration in Sephadex G-200. The efficiency of all steps was followed by protein determination and profiles in sodium dodecyl-sulphate polyacrylamide gel electrophoresis. Sheeps were immunized with purified IgG. The deep anti-rabbit IgG titre was evaluated by double immuno diffusion gel plates. The system rabbit serum vs sheep anti-rabbit IgG serum was used as precipitating system in radioimmunoassay of the retrovirus immunogens, in this case, p15 of avian leukosis virus. (author). 19 refs., 4 figs., 3 tabs.

  20. Clustering high dimensional data using RIA

    Energy Technology Data Exchange (ETDEWEB)

    Aziz, Nazrina [School of Quantitative Sciences, College of Arts and Sciences, Universiti Utara Malaysia, 06010 Sintok, Kedah (Malaysia)

    2015-05-15

    Clustering may simply represent a convenient method for organizing a large data set so that it can easily be understood and information can efficiently be retrieved. However, identifying cluster in high dimensionality data sets is a difficult task because of the curse of dimensionality. Another challenge in clustering is some traditional functions cannot capture the pattern dissimilarity among objects. In this article, we used an alternative dissimilarity measurement called Robust Influence Angle (RIA) in the partitioning method. RIA is developed using eigenstructure of the covariance matrix and robust principal component score. We notice that, it can obtain cluster easily and hence avoid the curse of dimensionality. It is also manage to cluster large data sets with mixed numeric and categorical value.

  1. Melancolia, memória e subjetividade

    OpenAIRE

    Giele Rocha Dorneles

    2015-01-01

    A proposta desta tese é estabelecer, através de uma perspectiva comparatista, relações entre três temas articuladores: a melancolia, a memória e a subjetividade, de modo a constituir entrelaçamentos possíveis, além de buscar indicar o modo como essas três temáticas são apresentadas e representadas em diferentes formas de expressão das artes, partindo de obras literárias - como “A maior flor do mundo” e “As pequenas memórias”, entre outras de José Saramago, e de autores como Charles Baudelaire...

  2. Camptocormia secundária à polimiosite

    OpenAIRE

    Melina Andrade Mattar; Joara Martins da Silva Gordo; Ari Stiel Radu Halpern; Samuel Katsuyuki Shinjo

    2013-01-01

    A camptocormia é uma doença postural caracterizada por flexão anormal da coluna toracolombar que surge na posição ereta, aumenta durante a caminhada e desaparece na posição supina. Na literatura, há descrição de apenas cinco casos de camptocormia secundária a miopatias inflamatórias idiopáticas. No presente relato de caso, descrevemos um paciente do sexo masculino, de 67 anos, com polimiosite há 18 anos, cursando com quadro compatível com camptocormia (estável e sem progressão do quadro clíni...

  3. A HISTÓRIA DA APPLE COMPUTER

    OpenAIRE

    2006-01-01

    Este artigo pretende mostrar os fatos e caminhos seguidos pela empresa de computadores Apple e seus fundadores no decorrer de sua história. Tem também o objetivo de relatar os desfechos e as grandes idéias que fizeram da Apple uma das maiores empresas fabricantes de softwares e computadores pessoais, desde sua criação em 1976.

  4. A HISTÓRIA DA APPLE COMPUTER

    Directory of Open Access Journals (Sweden)

    Gustavo Franco

    2006-06-01

    Full Text Available Este artigo pretende mostrar os fatos e caminhos seguidos pela empresa de computadores Apple e seus fundadores no decorrer de sua história. Tem também o objetivo de relatar os desfechos e as grandes idéias que fizeram da Apple uma das maiores empresas fabricantes de softwares e computadores pessoais, desde sua criação em 1976.

  5. RIA Beam Dynamics Comparing TRACK to IMPACT

    CERN Document Server

    Mustapha, Brahim; Ostroumov, Peter; Qiang, Ji; Ryne, Robert D

    2005-01-01

    In order to benchmark the newly developed beam dynamics code TRACK we have performed comparisons with well established existing codes. During code development, codes like TRANSPORT, COSY, GIOS and RAYTRACE were used to check TRACK's implementation of the different beam line elements. To benchmark the end-to-end simulation of the RIA driver linac, the simulation of the low-energy part (from the ion source to the entrance of the SC linac) was compared with PARMTEQ and found to agree well. For the simulation of the SC linac the code IMPACT is used. Prior to these simulations, the code IMPACT had to be updated to meet the special requirements of the RIA driver linac. Features such as multiple charge state acceleration, stripper simulation and beam collimation were added to the code. IMPACT was also modified to support new types of rf cavities and to include fringe fields for all the elements. This paper will present a comparison of the beam dynamics simulation in the RIA driver linac between the codes TRACK and I...

  6. Camptocormia secundária à polimiosite

    Directory of Open Access Journals (Sweden)

    Melina Andrade Mattar

    2013-08-01

    Full Text Available A camptocormia é uma doença postural caracterizada por flexão anormal da coluna toracolombar que surge na posição ereta, aumenta durante a caminhada e desaparece na posição supina. Na literatura, há descrição de apenas cinco casos de camptocormia secundária a miopatias inflamatórias idiopáticas. No presente relato de caso, descrevemos um paciente do sexo masculino, de 67 anos, com polimiosite há 18 anos, cursando com quadro compatível com camptocormia (estável e sem progressão do quadro clínico. A polimiosite é uma miopatia inflamatória idiopática caracterizada clinicamente por fraqueza muscular simétrica predominantemente proximal dos membros. Entretanto, sendo uma doença autoimune sistêmica, é plausível que ocorra acometimento de musculatura esquelética de forma difusa, incluindo a paravertebral, podendo manifestar-se com camptocormia.

  7. Contando uma bela história: a trajetória da ABEn - Bahia

    Directory of Open Access Journals (Sweden)

    Ieda Pessoa de Alcântara

    2001-06-01

    Full Text Available Trata este trabalho de contar a história da ABEn-Ba desde sua criação até os dias atuais, traçando um paralelo com a trajetória da ABEn Nacional e destacando os grandes marcos deste caminhar de cinqüenta e três anos. Fica evidente que a história repetiu-se ao colocar no destino da ABED/ABEn - Ba um começo relacionado à fundação da Escola de Enfermagem da Universidade Federal da Bahia, pois a vivência na Associação experimentada pelas professoras - enfermeiras em seus estados de origem, que as impulsionou para a criação da Seção Ba. História semelhante teve a Regional Feira de Santana, também formada após a criação do Curso da Universidade Estadual de Feira de Santana. Ao longo da sua história a ABEn - Ba vem primando por ser uma entidade democrática sempre atenta às lutas da categoria, na defesa da saúde da população brasileira, bem como, na busca do fortalecimento da Entidade.

  8. Antibodies and radioimmunoassay for phosphoserine, phosphothreonine and phosphotyrosine; Serologic specificities and levels of the phosphoamino acids in cytoplasmic fractions of rat tissues

    Energy Technology Data Exchange (ETDEWEB)

    Levine, L.; Gjika, H.B.; Van Vunakis, H. (Brandeis Univ., Waltham, MA (USA). Graduate Dept. of Biochemistry)

    1989-11-30

    For antibody production, the O-phosphorylated derivative of tyrosine,threonine, or serine was covalently linked to succinylated bovine albumin via the carbodiimide reaction. Each conjugate was then complexed with methylated bovine albumin for immunizationo of rabbits. To determine binding, the corresponding o-phosphorylated ({sup 3}H) amino acids were chemically synthesized. In addition, these {sup 3}H-phosphorylated derivatives were acylated (with succinic or acetic anhydride) to obtain ligands whose structures resemble those present in the immunogen. The acylated ligands bound to their respective antibodies more effectively: in some cases binding was about three orders of magnitude greater than their non-acylated counterparts. Radioimmunoassays were therefor developed using the N-succinyl-({sup 3}H) phosphoamino acids. When the unlabeled N-succinyl-phosphorylated amino acids were used as inhibitors in the homologous immune systems, 50% displacement of the labeled ligand was found with 0.06, 0.27 or 0.8 pmol of the tyrosine, threonine, or serine derivative, respectively. The antibodies were highly specific for the homologous hapten; the requirement for the phosphate group on the acylated amino acid was essentially absolute. The radioimmunoassays were used to quantitate the phosphoamino acids in cytoplasmic fractions of rat tissue extracts. (author). 37 refs.; 3 figs.; 5 tabs.

  9. Affinity-purified antibodies of defined specificity for use in a solid-phase microplate radioimmunoassay of human Tamm-Horsfall glycoprotein in urine.

    Science.gov (United States)

    Hunt, J S; McGiven, A R; Groufsky, A; Lynn, K L; Taylor, M C

    1985-05-01

    Rabbit antibodies to human Tamm-Horsfall glycoprotein (prepared by salt precipitation from normal urine) were purified by affinity chromatography using columns containing Tamm-Horsfall glycoprotein linked to CNBr-activated Sepharose 4B. The specificity of these antibodies was determined by analysis of their binding characteristics on Western blots of Tamm-Horsfall protein from sodium dodecyl sulphate/polyacrylamide gradient gels and comparison with the reactivity of monoclonal antibodies to this glycoprotein. Optimal conditions of adsorption to poly(vinyl chloride) microtitre plates were established such that these purified antibodies could be used in a solid-phase radioimmunoassay for the determination of urinary Tamm-Horsfall-glycoprotein concentration. The specificity of the immunoassay was confirmed by competitive inhibition of the urinary Tamm-Horsfall glycoprotein by purified freeze-dried material in solution. A standard curve obtained with this material showed the radioimmunoassay to have a sensitivity of at least 5 ng/ml, with linearity between 30 and 600 ng/ml. The mean coefficient of variation over the linear section of the curve was 11.3 +/- 2.2% (n = 13). The effects of dialysis and freezing of urine samples before determination of Tamm-Horsfall-glycoprotein concentrations were investigated and the mean 24 h urinary excretion rate in 60 normal donors was shown to be 84.9 +/- 44.1 mg.

  10. Memórias do Cárcere: história, memória e literatura

    Directory of Open Access Journals (Sweden)

    Kamilly Barros de Abreu Silva

    2008-01-01

    Full Text Available This work aims to articulatethe triad history / memory / literaturein the masterpiece composed byGraciliano Ramos, Memórias doCárcere (Memories of prision. Theconcept of literature of testimonyappears as the articulator of the triad,demanding a new treatment in theconsideration of the relations betweenhistory and memory and history andliterature. The testimony of the authorexposes the past in its fragmented face:in the fissures of history, many timesnot reached by the historian, anothermemory emerges – a memory capableof contributing to the movement ofhistory’s self-reflection.

  11. Geografia, História e História da Geografia

    Directory of Open Access Journals (Sweden)

    Antonio Carlos Robert Moraes

    2001-11-01

    Full Text Available Vale iniciar colocando a necessidade de bem caracterizar os dois campos disciplinares - história e geografia - e de bem equacionar suas relações e articulações. Em outros termos, cabe delimitar o universo de investigação enfocado e explicitar as motivações teóricas de seu estudo, evitando o fascínio do empirismo historiográfico, postura com possibilidade de ampla difusão num campo ainda pouco explorado em termos de levantamentos empíricos como o da história da geografia no Brasil. De imediato...

  12. Regulatory research / Pesquisa regulatória

    Directory of Open Access Journals (Sweden)

    Altair Souza de Assis

    2013-05-01

    Full Text Available We present in this paper the concept, importance, and scope of research into the regulatory framework of regulation and legislation, with a particular focus on legislation related to legal metrology. This study also describes a comparative analysis of the various forms of regulation and the associated regulatory research, with the ultimate goal of better defi ning the concept and to validate the need to have research groups within a country’s regulatory bodies. Based on this work, we conclude that regulatory research is a key factor in the success of any regulatory body’s activities. Such research helps to avoid the creation of absurd or impractical regulatory barriers to a country’s technological development, or worse, to permit “orphans,” that is, technologies that are outside regulatory control, as is currently the case. Indeed, for a country to have a robust technological infrastructure, especially if it is still a developing country, strong and competent regulatory control is essential. However, this must be balanced by an atmosphere that fosters continuous and consistent technological innovation, and such development must also be self-sustainable from economic, social, and environmental viewpoints. ------------------------------------------------ Apresenta-se neste trabalho o conceito, a importância e a abrangência da pesquisa regulatória no âmbito da regulação e da regulamentação, com foco particular na regulamentação relacionada à metrologia legal. Faz-se também uma análise comparativa entre as várias formas de regular e regulamentar, e as suas pesquisas regulatórias afi ns, tendo como meta principal situar melhor o conceito e validar a necessidade de se fazer pesquisa nos órgãos e agências regulatórias do país. Com base neste trabalho, concluímos que a pesquisa regulatória é um fator chave para o sucesso de qualquer plano de ação de regulação para os agentes regulatórios do país. Ela ajuda a

  13. Optimization of the synthesis of a high specific activity 125{sup I}-labelled hapten for radioimmunoassays; Optimizacion de un metodo de preparacion de un radiohapteno-125''I para ria de alta actividad especifica

    Energy Technology Data Exchange (ETDEWEB)

    Suraez, C.; Paz, D.; Simon, M. A.; Romero del Hombrebueno, B.

    1994-07-01

    In this first report it is described the synthesis, separation and purification of the 2-radioiodinated histamine- I-labelled histamine by a mixed anhydride reaction. About 75% incorporation of I{sup -}125, from Na{sup 1}25I, was achieved with a molecular ratio of 1:1 mixed anhydride:histamine. The radiochemical purity of the conjugate by TLC was > 99% and its theoretical specific activity, 3850 {mu}Ci/{mu}g. Dissolved in ethanol and held at -20 degree centigree under darkness decomposition on storage didn't exceed 1% per month. (Author) 13 refs.

  14. Application of solid-phase radioimmunoassay in determining antibodies to Aujeszky's disease virus in blood serum of vaccinated pigs

    Energy Technology Data Exchange (ETDEWEB)

    Rodak, L.; Smid, B.; Valicek, L. (Vyzkumny Ustav Veterinarniho Lekarstvi, Brno-Medlanky (Czechoslovakia))

    1983-11-01

    In the blood sera of pigs vaccinated with inactivated vaccines manufactured by three different manufacturers the RIA method was used to determine the specific antibodies to the virus of Aujeszky's disease. In certain groups of vaccinated pigs the results of the RIA examination are unfavourably affected by the bond of antibodies to the cellular antigenous determinants. This proves that following vaccination antibodies are formed not only against the viral antigen but also against the antigens of cells on which the vaccination virus is propagated. These shortcomings are eliminated by the use of suitable cellular cultures for the preparation of viral and control antigens. Antigens are applicable for RIA and for ELISA examinations of blood sera of infected and vaccinated pigs. The advantages are described of the RIA and ELISA methods as compared with the virus neutralization test.

  15. Os teares da memória

    Directory of Open Access Journals (Sweden)

    Ana Luísa Amaral

    2012-10-01

    Full Text Available Partindo da ideia de que a verdade do texto e a verdade da vida não são coincidentes, mas que a uni‑las está um rasto de verdade, forçosamente diverso daquilo a que se convencionou designar por fingimento, debato aqui a questão de uma identidade de mulher no texto e ainda o papel que a memória desempenha na construção de identidades mais latas, as que ao humano dizem respeito.

  16. Psicologia social comunitária profissional

    OpenAIRE

    2007-01-01

    O presente texto aborda questões relativas à construção da psicologia social comunitária no Brasil e as interfaces deste processo histórico com a formação profissional. Para tanto, apóia-se em dados sobre o contexto histórico e social brasileiro no decorrer da efetivação das práticas psicológicas em comunidades. Finalmente, o estudo propõe reflexões quanto ao descompasso entre a formação e os fenômenos sociais contemporâneos.

  17. Meningeoma da goteira olfatória

    OpenAIRE

    Tella Jr Osvaldo Inácio de; Paiva Neto Manoel Antonio; Herculano Marco Antonio; Faedo Neto Atílio

    2006-01-01

    Meningeomas da goteira olfatória representam 8-18% dos meningeomas intracranianos e geralmente são diagnosticados quando alcançam grande tamanho. Relatamos uma série de 13 casos consecutivos de meningeomas de goteira olfativa operados nos Hospitais São Paulo (UNIFESP) e Professor Edmundo Vasconcelos no período de 1995 a 2003, estudados retrospectivamente quanto ao quadro clínico, resultados cirúrgicos e complicações. Os pacientes foram submetidos a ressecção cirúrgica destes tumores pela via ...

  18. História da ultra-sonografia intra-operatória

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    Machado Márcio Martins

    2002-01-01

    Full Text Available A ultra-sonografia intra-operatória foi realizada especialmente a partir de 1960, com alguns relatos de experiências iniciais nos anos 50. Inicialmente foram avaliados tumores cerebrais, posteriormente estudando-se também cálculos de vias biliares e cálculos renais. Entretanto, a ultra-sonografia intra-operatória em modo A ou modo B estático não adquiriu grande aceitação no meio médico. Não obstante, os primeiros estudos forneceram as bases para o desenvolvimento da moderna ultra-sonografia intra-operatória, com a utilização dos equipamentos ultra-sonográficos em modo B em tempo real. Os autores discorrem sobre a utilização da ultra-sonografia intra-operatória desde o seu início até os dias atuais.

  19. A novel microtiter plate radioimmunoassay of insulin autoantibody%胰岛素自身抗体微量平板放射免疫法的建立与应用

    Institute of Scientific and Technical Information of China (English)

    黄干; 李璋巍; 金河来; 王霞; 王建平; 周智广

    2009-01-01

    Objective Insulin autoantibody (IAA) is known to exist in sera of type 1 diabetes mellitus (T1DM) patients and pre-T1DM individuals. The aim of this study was to establish a novel microtiter plate radioimmunoassay (RIA) for IAA and evaluate its clinical value. Methods Diluted 125Ⅰ-insulin was mixed with 5 ul serum samples in a 96-well microtiter plate and then incubated for 72 h on an orbital plate shaker (4℃). The immunocomplexes were transferred to another protein a coated Millipore plate, and then the plate was washed with Tri-Buffered Saline Tween-20 (TBT) buffer. Counts per minute (CPM) was measured with liquid scintillation and luminescence counter. The positive cut-off point of IAA index was defined as ≥0.06 based on the 99-percentile of the distribution in 317 healthy individuals. The specificity and sensitivity of the assay were calculated from the samples provided by the fourth Diabetes Autoantibodies Standardization Program (DASP 2005). The IAA levels were determined in 71 T1 DM and 551 newly diagnosed type 2 diabetes (T2DM) patients, and 317 healthy controls. The t test, non-parametric test, x2 test and linear correlation analysis were performed on the data using SPSS 11.5 software. The concordance rate was estimated with Kappa value. Results (1) The optimized testing condition was described as 2×104 CPM of 125Ⅰ-insulin, 5 ul serum sample and slowly horizontal shaking for 72 h. (2) The intra-assay CV was 4.8%-8.9% and inter-assay CV was 6.4%-10.5%. Based on DASP 2005 samples, the specificity and sensitivity of the assay were 97% (97/100) and 50% (25/50), respectively. Ninety-six serum samples with different IAA levels were selected and tested to compare between our new method and a domestic IAA RIA kit. The results showed that the IAA indices from the two methods were positively correlated (r= 0.678, P0.05). Conclusions Our proposed microtiter plate RIA method for IAA is highly sensitive and specific, likely to be feasible for clinical

  20. Oxytocin determination by radioimmunoassay. III. Improvement to subpicogram sensitivity and application to blood levels in cyclic cattle

    Energy Technology Data Exchange (ETDEWEB)

    Schams, D. (Insitut fuer Physiologie der Sueddeutschen Versuchs- und Forschungsanstalt der Technischen Universitaet Muenchen, FRG)

    1983-01-01

    An improved RIA for measurement of oxytocin in blood is described by using an extraction method with SEP-PAK C/sub 18/ cartridges, which allows also concentration of the sample, a new antiserum with a higher sensitivity to standard oxytocin and preparation of the standard curve in buffer. The lower limit of assay sensitivity was 0.25 pg/tube, corresponding to 0.25-1.0 pg/ml plasma depending on the amount of plasma extracted. Hence, it was no problem to measure oxytocin basal concentrations in peripheral blood in the range of 0.6-4 pg/ml plasma depending on the stage of the oestrous cycle. The highest oxytocin concentrations occurred during the early and mid-luteal phase. The method has been applied also for samples from women, sheep, pigs and horses. Mean (+-SD) recovery of oxytocin added to plasma or only buffer after extraction was 71.3+-8,1%, and the coefficient of variation (CV) = 11.4% (n = 27 assays). The intra-assay CV of two control samples was 7.9+-2.8 and 7.8+-2.4% (n = 17 assays). The inter-assay CV of 5 control samples with low and high oxytocin concentrations varied between 10.8+-17.3% (n = 25 assays). The 50% intercept was 2.5+-0.3 pg, CV = 11.3% (n = 29 assays).

  1. História da educação: qual história?

    Directory of Open Access Journals (Sweden)

    José Licínio Backes

    2012-06-01

    Full Text Available  Este artigo reflete sobre uma concepção de história que privilegie uma história da educação que leve em conta as inúmeras diferenças que existem numa sociedade. A partir de Chartier. Busca­-se entender que a diferença está para além das da ordem de fortuna (raça, gênero, crença. Junto com este entendimento defende-se também uma história que englobe as vozes marginais, que não absolutize suas verdades e leve em conta que as pessoas não agem/reagem da mesma forma às imposições de uma estrutura, dando espaço para sujeitos singulares e diferentes.Palavras-chave: história da educação, Chrtier, história.   Abstract This article reflects about a conception of history which privileges a history of education that considers the innumerous differences existing in society. Departing from Chartier, we try to understand that the difference exists beyond the order of fortune (it includes race, gender, faith. Together with this understanding, we also advocate a history that comprises the marginal voices. which does not consider as absolute its truths and takes into account that different people don't act/react in the same way to the impositions of a structure, giving space to single and different subjects. Keywords: history of education, Chartier, history.   

  2. A biotecnologia na agropecuária

    Directory of Open Access Journals (Sweden)

    João Lúcio de Azevedo

    1986-11-01

    Full Text Available This review reports the use of biotechnology in agriculture and animal husbandry. The main techniques and the most important products both in experimental stage or in commercial use are reported. The work intends to give a global vision to the theme, chronologically and spacely, showing the main lines of research and what has been obtained in this area. The techniques and products that are related to genetics and breeding are emphasized. Revisão Bibliográfica do emprego da biotecnologia na agropecuária, mostrando um levantamento das técnicas mais promissoras e de seus principais produtos, tanto em fase experimental como em uso comercial. Pretende-se dar uma visão global do assunto, cronológica e espacialmente, não se prendendo à detalhes, mostrando, em Unhas gerais, o que foi feito e o que se faz, atualmente, em biotecnologia. As técnicas e produtos que influenciam a área de genética e melhoramento genético na agropecuária, são enfatizadas.

  3. FONTES PRIMÁRIAS: SABERES EM MOVIMENTO

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    CAMPOS Edson Nascimento

    1997-01-01

    Full Text Available Este artigo trata as fontes primárias à luz de uma epistemologia que submete a investigação e a pesquisa à ação desconstrutora dos sujeitos: as fontes, por isso, saem da posição de "ponto primacial" para a de "ponto relacional". E, aí, a linguagem se transforma em relação de intertextualidade, pois a contradição marca o sentido da interdependência e, nisso, a significação põe a força da instabilidade na relação do sujeito com o objeto. Este, aliás, aberto à abordagem múltipla do real, integra a relação de interdisciplinaridade, e, assim, vêm surgindo, por exemplo, explicações iluminadoras com as articulações de Educação, Pedagogia, Linguagem, História.

  4. Radioimmunoassay of IgG and IgM rheumatoid factors reacting with human IgG. [/sup 125/I tracer technique

    Energy Technology Data Exchange (ETDEWEB)

    Carson, D.A.; Lawrance, S.; Catalano, M.A.; Vaughan, J.H.; Abraham, G.

    1977-07-01

    Although IgG rheumatoid factor may play a central role in the pathogenesis of rheumatoid arthritis, previously there have been no precise methods for its specific measurement in serum and synovial fluid. This paper describes a solid phase radioimmunoassay for the independent quantification of IgM and IgG rheumatoid factor reacting with the Fc fragment of human IgG. As measured by this assay, serum IgG rheumatoid factor levels differed significantly between patients with seropositive and seronegative rheumatoid arthritis and normal control subjects. In addition, several sera and joint fluids from patients with seropositive rheumatoid arthritis, even without vasculitis, were shown by gel chromatography to have acid-dissociable complexes of IgG rheumatoid factor suggestive of IgG-IgG dimer or trimer formation.

  5. Validity of a radioimmunoassay for serum and pituitary prolactin in adult male rats. Effects of bromocriptine and thyrotropin-releasing hormone

    Energy Technology Data Exchange (ETDEWEB)

    Andre, M. Grizard, G.; Boucher, D. (Faculte de Medecine, 63 - Clermont-Ferrand (France))

    1981-01-01

    Validity of a radioimmunoassay for rat prolactin (PRL) in serum and pituitary is analysed in adult male rats. Data are presented bearing on the accuracy, precision and sensitivity of the method. Serum levels and pituitary content are respectively ranged from 2,56 to 28,03 ng PRL RP/sub 2/ ml/sup -1/ and from 7,36 to 21,44 ..mu..g PRL RP/sub 2/ per gland in intact animals. Treatment with bromocriptine (10 days) results in a decrease of serum PRL levels and pituitary PRL contents. In progesterone-estradiol benzoate pretreated rats, serum PRL levels are increased 20 min after the injection of TRH.

  6. Solid-phase radioimmunoassay for measurement of circulating antibody titres to wheat gliadin and its subfractions in patients with adult coeliac disease

    Energy Technology Data Exchange (ETDEWEB)

    Ciclitira, P.J.; Ellis, H.J. (Guy' s Hospital, London (UK)); Evans, D.J. (Hammersmith Hospital, London (UK))

    1983-08-26

    A solid-phase radioimmunoassay for the measurement of circulating antibody titres to wheat gliadin is described. Using this assay, the authors have measured antibody titres to unfractionated gliadin in normal healthy controls, in coeliac patients on a gluten-free or a normal diet, and in patients with ulcerative colitis and Crohn's disease. High titres of antibodies to unfractionated gliadin were observed only in the patients with untreated coeliac disease. Antibody titres to ..cap alpha.., ..beta.., ..gamma.. and ..omega.. gliadin subfractions were measured in patients with untreated coeliac disease and compared with titres in normal controls. Patients with untreated coeliac disease had higher antibody titres to the gliadin subfractions. No specific pattern of circulating antibody titres to gliadin subfractions was observed in the untreated coeliac patients which would provide a diagnostic profile. These results suggest shared antigenicity between the gliadin subfractions.

  7. Development of a radioimmunoassay for Pro-Leu-Gly-NH/sub 2/ (PLG or MIF-I). Evidence that PLG is not present in rat brain

    Energy Technology Data Exchange (ETDEWEB)

    Manberg, P.J.; Youngblood, W.W.; Kizer, J.S. (North Carolina Univ., Chapel Hill (USA). School of Medicine)

    1982-06-10

    Pro-Leu-Gly-NH/sub 2/ (PLG), which is the C-terminal tripeptide tail of oxytocin, has been reported to possess melanocyte-stimulating hormone (MSH)-release-inhibiting activity. Although it has been isolated from bovine hypothalamus, little is known about the CNS distribution of this peptide in other species. In this report the development of a radioimmunoassay is described which can be used to measure both PLG and oxytocin following chromatographic separation by high pressure liquid chromatography (HPLC). Using this method the authors demonstrate the presence of any endogenous PLG in rat hypothalamus, preoptic area, pituitary, or eye tissue. However, synthetic PLG, which is added to tissue homogenates as an internal standard, is consistently recovered from all areas. It is concluded that the PLG tripeptide is not present in the rat brain and thus cannot be the physiological regulator of MSH secretion.

  8. Antibody response to the lipopolysaccharide and protein antigens of Salmonella typhi during typhoid infection. II. Measurement of intestinal antibodies by radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Chau, P.Y.; Tsang, R.S.W.; Lam, S.K. (Hong Kong Univ.); La Brooy, J.T.; Rowley, D. (Adelaide Univ. (Australia))

    1981-12-01

    Antibodies to the lipopolysaccharide (LPS) and protein antigens of S. typhi in secretions of small intestine obtained from 12 typhoid patients, four typhoid carriers and 16 non-typhoid control subjects were measured by a solid-phase radioimmunoassay technique using /sup 125/I labelled anti-immunoglobulin antibody. Intestinal secretions obtained from typhoid patients as a group had significantly higher anti-LPS and anti-protein antibodies than those from the control group. These antibodies were both IgM and IgA classes. There was no correlation between the IgM or IgA antibody levels in serum and those in the intestinal secretions. In the intestinal secretions obtained from typhoid carriers, on the other hand, only IgA-class antibodies to the LPS and protein antigens of S. typhi were present at high levels.

  9. Relation between enzyme-linked immunosorbent assay and radioimmunoassay for detection of antibodies to the capsular polysaccharide of Haemophilus influenzae type b

    Energy Technology Data Exchange (ETDEWEB)

    Kristensen, K. (Streptococcus Department, Statens Seruminstitut, Copenhagen (Denmark)); Weis Bentzon, M. (Department of Biostatistics, Statens Seruminstitut, Copenhagen (Denmark))

    1992-01-01

    The measurement of antibodies to the capsular polysaccharide (PRP) of Haemophilus influenzae type b (Hib) is important because vaccines inducing such antibodies are now available. We developed and evaluated an enzyme-linked immunosorbent assay (ELISA) for detection of these antibodies based on direct coating of the plates with tyraminated PRP. The assay fulfilled the requirements for parallel line assays; it was sensitive, specific, and reproducible with a coefficient of variation between days of 19%. Results from the ELISA were compared with results from radioimmunoassay and a correlation coefficient of 0.93 was found. Results obtained by the two methods were proportional and the relation was indepenedent of the antibody level. The relation between them was also unaffected by the contribution of different antibody isotypes, indicating that these were measured to the same extent by both methods. ELISA employing direct coating of the plates with tyraminated PRP represents a useful alternative for detection of antibodies when studying immunogenicity of Hib vaccines. (au).

  10. Expression of blood group I and i active carbohydrate sequences on cultured human and animal cell lines assessed by radioimmunoassays with monoclonal cold agglutinins

    Energy Technology Data Exchange (ETDEWEB)

    Childs, R.A.; Kapadia, A.; Feizi, T. (Clinical Research Centre, Harrow (UK))

    1980-05-01

    Human monoclonal anti-I und anti-i antibodies, reactive with known carbohydrate sequences, have been used as reagents to quantitate (by radioimmunoassay) and visualize (by immunofluorscence) the expression of the various blood group I and i antigenic determinants in a variety of cultured cell lines commonly used in laboratory investigations. It has been shown that the antigens they recognize are widely distributed on the surface of human and animal cell lines, expressed in varying amounts in different cell lines and on individual cells within a given cell line. In two cell lines, a transformation-associated increase in the expression of I antigen was observed. Because of their precise specificity for defined carbohydrate chain domains, these autoantibodies have become valuable reagents in biological chemistry.

  11. Expression of blood group I and I active carbohydrate sequences on cultured human and animal cell lines assessed by radioimmunoassays with monoclonal cold agglutinins

    Energy Technology Data Exchange (ETDEWEB)

    Childs, R.A.; Kapadia, A.; Feizi, T.

    1980-05-01

    Human monoclonal anti-I and anti-i, reactive with known carbohydrate sequences, have been used as reagents to quantitate (by radioimmunoassay) and visualize (by immunofluorescence) the expression of the various blood group I and i antigenic determinants in a variety of cultured cell lines commonly used in laboratory investigations. It has been shown that the antigens they recognize are widely distributed on the surface of human and animal cell lines, expressed in varying amounts in different cell lines and on individual cells within a given cell line. In two cell lines, a transformation-associated increase in the expression of I antigen was observed. Because of their precise specificity for defined carbohydrate chain domains, these autoantibodies have become valuable reagents in biological chemistry.

  12. A double antibody radioimmunoassay for measurement of IgG, IgA and IgM synthesized by human lymphocytes in vitro.

    Directory of Open Access Journals (Sweden)

    Asano,Taro

    1981-11-01

    Full Text Available To investigate cellular interactions between human T and B lymphocytes in various diseases, we established a technique to prove terminal differentiation of B lymphocytes into immunoglobulin synthesizing and secreting cells. We also established a double antibody radioimmunoassay to measure the amount of IgG, IgA and IgM synthesized and secreted in culture supernatants. Purified immunoglobulins were obtained from sera of patients with myeloma or macroglobulinemia. The peripheral blood lymphocytes from 25 normal individuals had the geometric mean synthetic rates of 1886 ng for IgG, 1607 ng for IgA and 1173 ng for IgM per 1 X 10(6 cells when cultured for nine days in the presence of pokeweed mitogen. The method is simple and sensitive, and is thought to be useful for examining human lymphocyte function in vitro.

  13. Application of ovine luteinizing hormone (LH) radioimmunoassay in the quantitation of LH in different mammalian species. [/sup 125/I tracer technique

    Energy Technology Data Exchange (ETDEWEB)

    Millar, R.P.; Aehnelt, C.

    1977-09-01

    A sensitive double antibody radioimmunoassay has been developed for measuring luteinizing hormone (LH) in various African mammalian species, using rabbit anti-ovine LH serum (GDN 15) and radioiodinated rat LH or ovine LH. Serum and pituitary homogenates from some African mammals (hyrax, reedbuck, sable, impala, tsessebe, thar, spring-hare, ground squirrel and cheetah, as well as the domestic sheep, cow and horse and laboratory rat and hamster) produced displacement curves parallel to that of the ovine LH standards. The specificity of the assay was examined in detail for one species, the rock hyrax. Radioimmunoassay and bioassay estimates of LH in hyrax pituitaries containing widely differing quantities of pituitary hormones were similar. In sexually active male hyrax mean plasma LH was 12.1 ng/ml and pituitary LH 194 ..mu..g/gland, but in sexually quiescent hyrax mean plasma LH was 2.4 ng/ml and mean pituitary LH 76 ..mu..g/gland. Intravenous injection of 10 ..mu..g of luteinizing hormone releasing hormone increased mean LH levels in hyrax from 0.9 ng/ml to 23.2 ng/ml by 30 min. Conversely, im injection of 250 ..mu..g testosterone induced a fall in LH levels in male hyrax from 1.7 ng/ml to 0.7 ng/ml 6 h after administration. Although the specificity of the assay for quantitating plasma LH in other species was not categorically established, there was a good correlation between plasma LH concentration and reproductive state in the bontebok, impala, spring-hare, thar, cheetah, domestic horse and laboratory rat, suggesting the potential use of the antiserum in quantitating LH in a variety of mammalian species.

  14. Aptidão cardiorrespiratória em adolescentes

    Directory of Open Access Journals (Sweden)

    A. Pelegrini

    2017-09-01

    Conclusão: Conclui‐se que a maioria dos adolescentes apresentam níveis inadequados de aptidão cardiorrespiratória, e a associação entre composição corporal e aptidão cardiorrespiratória inadequadas independe dos aspectos sociodemográficos dos adolescentes.

  15. ESCRITA E HISTÓRIAS DE TOXICOMANIAS

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    Sandra Djambolakdjian Torossian

    2007-08-01

    Full Text Available O presente artigo se constrói na interface entre reflexões sobre a escrita e a temática da subjetividade. Para isso, apresenta-se uma análise realizada a partir de textos escritos por pessoas que sofrem de toxicomanias durante seu processo de tratamento. Nesta trajetória, traçam-se algumas considerações sobre a noção de subjetividade para a Psicanálise, discutindo o entrelaçamento entre o processo de escrita e a construção subjetiva. Focalizam-se, ainda, as possibilidades de invenção de subjetividade tendo a escrita como um dos possíveis dispositivos de tratamento.

  16. Museu, Memória e Poder

    Directory of Open Access Journals (Sweden)

    Renata Andreoni

    2011-12-01

    Full Text Available Este trabalho pretende desenvolver uma apreciação referente às imbricações entre Museu, Poder e Memória dentro da esfera empresarial. Objetiva-se compreender como a prática memorialística é utilizada na construção de um discurso pela busca de uma aura histórica legitimadora. No contexto da Hipermodernidade (Lipovetsky,2004, para problematizar a reflexão sobre os museus empresariais, destacaremos o posicionamento de Baudrillard em relação aos processos de simulação e branqueamento de um passado sem máculas.

  17. Em nome da memória

    Directory of Open Access Journals (Sweden)

    Maria Auxiliadora de Almeida Cunha Arantes

    Full Text Available Este texto apresenta cinco breves estórias sobre o assassinato de psicólogas e de estudantes de Psicologia que se engajaram na luta de resistência. Todas foram executadas durante a ditadura civil militar, na década de 70, e um dos casos contou com a participação da Operação Condor. As práticas extremamente cruéis de tortura, o ocultamento de provas, a simulação de morte por suicídio, o abandono de corpos em locais públicos na tentativa de desresponsabilizar os executores tornaram-se evidentes nos laudos necroscópicos posteriormente acessados e/ou em depoimentos de advogados e familiares. A intenção do texto é permitir aos psicólogos e psicólogas o acesso às histórias de jovens mulheres que também escolheram a Psicologia como profissão e que foram impedidas precocemente de viver e de realizar projetos próprios. Disponibilizar informações sobre esses fatos e oferecer esclarecimentos sobre a prática da tortura convida à participação cidadã no processo de aperfeiçoamento do Estado Democrático de Direito que avança em nosso país com a instalação da Comissão da Verdade.

  18. Aprendizado e memória Learning and memory

    OpenAIRE

    Paul Lombroso

    2004-01-01

    A memória é dividida de duas grandes formas: explícita e implícita. O hipocampo é necessário para a formação das memórias explícitas, ao passo que várias outras regiões do cérebro, incluindo o estriado, a amígdala e o nucleus accumbens, estão envolvidos na formação das memórias implícitas. A formação de todas as memórias requer alterações morfológicas nas sinapses: novas sinapses devem ser formadas ou antigas precisam ser fortalecidas. Considera-se que essas alterações reflitam a base celular...

  19. Enxerto livre de artéria mamária interna: resultados imediatos e tardios

    OpenAIRE

    1989-01-01

    Em nosso Serviço, temos dado preferência pelo uso da artéria mamária interna na cirurgia de revascularização miocárdica, utizizando-a em 93% dos pacientes com menos de 65 anos, durante este último ano. Quando, por motivos anatômicos, torna-se impossível seu uso in situ, temos lançado máo de sua utilização como enxerto livre. De abril de 1986 a setembro de 1988, este procedimento foi realizado em 58 pacientes submetidos à cirurgia de revascularização miocárdica isolada e pela primeira vez (for...

  20. A história estrutural: trajetória, conceitos e aplicabilidade

    Directory of Open Access Journals (Sweden)

    Júlia Silveira Matos

    2010-10-01

    Full Text Available Na França, o movimento posteriormente intitulado de Annalistes exerceu uma verdadeira hegemonia no processo de renovação dos métodos e problemáticas da História. Entretanto, suas reflexões permaneceram muito mais no campo da epistemologia do que propriamente da teoria histórica. Somente as gerações posteriores a Marc Bloch e Lucien Febvre, chamados os pais fundadores dos Annales, buscaram a sistematização de metodologias e esboços de uma possível teoria, inspirada por diversas ciências – lingüística, geografia, psicologia, antropologia, sociologia, entre outras. A partir dessa percepção, propomos no presente artigo, debruçados sobre o texto-manifesto de Fernando Braudel “História e Ciências Sociais”, a discussão do desenvolvimento das análises de cunho estrutural na história, não apenas enquanto teoria, mas principalmente enquanto método, de forma que possamos perceber as possibilidades de aplicação da abordagem estrutural na pesquisa histórica.

  1. A PROJEÇÃO IDENTITÁRIA NO "MURAL DE HISTÓRIAS" DE MAYSA

    Directory of Open Access Journals (Sweden)

    Guilherme Moreira Fernandes

    2011-06-01

    Full Text Available Neste artigo, fazemos uma breve reflexão sobre os mecanismos de projeção e formação identitárias presentes no espaço "Mural de Histórias", do portal da minissérie Maysa: quando fala o coração, exibida em 2009 pela TV Globo. A metodologia da análise de conteúdo nos permitiu categorizar as várias facetas da cantora comentadas pelos internautas. Utilizando os estudos de Aluizio Trinta sobre os mecanismos de projeção-identificação, pudemos inferir que parcela dos internautas se dispôs a refletir sobre a identidade deles. Também percebemos que as múltiplas plataformas criadas pela emissora permitem uma aproximação maior do público e um espaço para manifestações de alegrias e angústias.

  2. Memória e história: Hannah Arendt em diálogo com Walter Benjamin

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    Magalhães, Marionilde Brepohl de

    2006-01-01

    Full Text Available A autora tem como proposta refletir sobre o papel da memória e da História em nossa cultura contemporânea, através de uma aproximação ao pensamento de Hannah Arendt e Walter Benjamin

  3. Radioimmunoassay and enzyme-linked immunoassay of antibodies to the core protein (P24) of human T-lymphotropic virus (HTLV III). [Acquired immunodeficiency syndrome (AIDS)

    Energy Technology Data Exchange (ETDEWEB)

    Neurath, A.R.; Strick, N.; Sproul, P.

    1985-05-01

    Human T-cell lymphotropic viruses designated HTLV III or LAV are considered to represent the causative agents of the acquired immunodeficiency syndrome (AIDS). Therefore a simple direct RIA or ELISA method for antibodies to distinct epitopes of HTLV III/LAV structural components would be of great value. The authors describe RIA and ELISA assays which obviate the need for purified virus or virus proteins, do not utilize infected cells and thus do not diminish the source for continuous production of viral antigens and are specific for a major core protein of HTLV III/LAV.

  4. Reportagem, memória e história no jornalismo brasileiro

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    Alexandre Bergamo

    2011-08-01

    Full Text Available O presente artigo procura demonstrar de que modo mudanças recentes no exercício da profissão de jornalista estão sendo capazes de redefinir a forma como esses profissionais olham para si mesmos no presente e, consequentemente, para seu passado, para sua própria "história". Para tanto, me apoio em minha própria e em outras etnografias do jornalismo - em particular do espaço da redação - na especificidade da "notícia" e, principalmente, da "reportagem" enquanto "documento" histórico, discussão necessária para a compreensão da vinculação que se estabelece entre a "memória jornalística" e a "memória nacional"; e nos efeitos que a imposição de um "estilo jornalístico", a exigência do diploma e a reorganização do mercado de trabalho tiveram - e têm - sobre a organização e a escrita, cada vez mais sistematizada, das trajetórias consideradas exemplares para a área.The present article explores how recent changes in journalism have redefined the way in which the area's professionals see themselves in the present and so how they see their past, their own 'history.' I draw from my own and other ethnographies of journalism, particularly those focused on the editorial office, in order to examine the specificity of 'news' and especially 'reporting' as a historical 'document,' a discussion essential to understanding the link between 'journalistic memory' and 'national memory.' The text also discusses the effects that the imposition of a 'journalistic style,' the need for formal qualifications and the reorganization of the work market have had - and continue to have - on the increasingly systemized organization and description of careers now considered emblematic in the profession.

  5. Radioimmunoassay for 3,5-diiodothyronine and evidence for dependence on conversion from 3,5,3'-triiodothyronine

    Energy Technology Data Exchange (ETDEWEB)

    Pangaro, L.; Burman, K.D.; Wartofsky, L.; Cahnmann, H.J.; Smallridge, R.C.; O' Brian, J.T.; Wright, F.D.; Latham, K.

    1980-06-01

    The present report describes a RIA for 3,5-diiodothyronine (3,5T/sub 2/) which uses inner ring-labeled 3,5-(/sup 125/I)T/sub 2/ as the ligand and has a lower limit of detectability of 0.5 ng/dl. Cross-reaction was 0.14% with T/sub 3/, less than 0.001% with T/sub 4/, 1.2% with 3,3',5-triiodothyroacetic acid, and 6.1% with 3,5-diiodothyroacetic acid. No cross-reaction was detectable for iodothyronines within their physiological ranges. Intraassay variation ranged from 2.2 to 7.8%, and interassay variation ranged from 12.7 to 14%. The mean (+-SE) serum 3.5T/sub 2/ concentration in 70 normal subjects was 4.3 +- 0.2 ng/dl. The mean (+-SE) 3.5T/sub 2/ in 14 hyperthyroid patients was increased to 18.4 +- 2.3 ng/dl (P < 0.001), and all but 1 patient had an elevated level. In 10 hypothyroid patients the mean (+-SE) 3,5T/sub 2/ level was decreased to 1.4 +- 0.3 ng/dl (P < 0.001). In 4 patients, levels overlapped with the normal range. In 4 hypothyroid subjects treated with L-T/sub 1/, 3,5T/sub 2/ levels were normal, suggesting that the majority of 3,5T/sub 2/ originates from extrathyroidal conversion from T/sub 3/. Studies in fasting obese subjects demonstrated that serum 3,5T/sub 2/ (mean +- SE) levels fell from 3.4 +- 0.3 to 2.5 +- 0.7 ng/dl during fasting. This fall was significant (P < 0.001) and in parallel with the fall in T/sub 3/ levels of 182 +- 20 to 126 +- 12 ng/dl. In fasting subjects given 100 ..mu..g oral L-T/sub 3//day T/sub 3/ levels rose from 138 +- 11 to 362 +- 26 ng/dl. 3,5T/sub 2/ levels (corrected for cross-reaction and for contamination of oral T/sub 3/ with 3,5T/sub 2/) rose from 2.2 +- 0.7 to 6.4 +- 1.0 ng/dl. In fasting subjects given 25 ..mu..g oral L-T/sub 3//day, T/sub 3/ levels fell from 165 +- 5.1 to 139 +- 6.9 ng/dl. Corrected 3,5T/sub 2/ levels changed from 3.7 +- 0.4 to 2.5 +- 0.3 ng/dl. Neither change were significant.

  6. História da Escola Itinerante Caminhos do Saber

    OpenAIRE

    Paulo Roberto Urbinatti Urquiza

    2009-01-01

    O presente estudo tem como objetivo sistematizar a história de constituição da Escola Itinerante Caminhos do Saber, situada no Acampamento Maila Sabrina, Brigada Che Guevara, em Ortigueira no Paraná a partir de fontes primárias. O estudo foi predominantemente qualitativo, tomando como fontes primárias a análise de documentos tais como produção bibliográfica do próprio Movimento, trabalhos de pesquisa e legislações pertinentes à temática. A originalidade da pesquisa é definida pela utilização ...

  7. As normas de competência tributária

    OpenAIRE

    Follador, Guilherme Broto

    2013-01-01

    Resumo: O objetivo principal deste trabalho é analisar, criticamente, o conceito de competência tributária e as propostas doutrinárias de formalização das normas de competência tributária, expondo, a partir das lições colhidas sobre o tema, na Teoria Geral do Direito, os principais problemas encontrados nas tentativas de reconstruílas como típicas normas prescritivas. No primeiro capítulo, o estudo é dedicado ao exame das noções de "norma" e de "norma jurídica", e tem por principal finalidade...

  8. Trajetória dos estudos de velhice no Brasil

    OpenAIRE

    Barros,Myriam Moraes Lins de

    2006-01-01

    Este artigo pretende traçar a trajetória das pesquisas antropológicas sobre velhice realizadas no Brasil desde a década de 1970, e apresentar as discussões atuais e seus desdobramentos em projetos de investigação. Dentro do campo de estudos sobre velhice foram escolhidas algumas temáticas: identidade social, memória, trajetória de vida, sociabilidade, espaço urbano, trabalho e aposentadoria, gênero, família e corpo. O recorte histórico procurou apontar as questões teóricas que foram sendo inc...

  9. Tratamento cirúrgico da artéria coronária direita intra-atrial

    Directory of Open Access Journals (Sweden)

    José Carlos R. IGLÉZIAS

    1997-07-01

    Full Text Available A localização intracavitária da artéria coronária é rara. Segundo Ochsner & Mills (1, ela ocorreu com a artéria coronária direita em 0,9% e com o ramo interventricular em 0,2%. A localização da lesão e as condições patológicas relacionadas ao comprimento e diâmetro da coronária podem auxiliar na exposição da coronária intracavitária para uma revascularização apropriada. Freqüentemente os cirurgiões não estão habituados com a posição intracavitária e, durante a dissecção, podem abrir uma câmara cardíaca onde o vaso penetra. Os problemas que podem advir dai são a entrada de ar para a cavidade, a dificuldade na exposição do vaso, o sangramento e a obstrução da artéria durante o fechamento da miotomia. São relatados os casos de 3 pacientes que necessitaram de revascularização cirúrgica do miocárdio e que apresentavam a artéria coronária direita intracavitária. A localização e o comprimento da porção intracavitária da artéria auxiliam no manejo intra-operatório. A técnica utilizada para o fechamento da cavidade variou entre a anastomose na posição intracavitária com o fechamento da miotomia ao redor do enxerto (Figura 1, a liberação da artéria coronária para uma posição superficial (Figura 2, seguida da anastomose e fechamento da cavidade com sutura simples, feita subepicárdica.An intracavitary location of a coronary artery is rare in our surgical experience with revascularization. This variant has occured in the right coronary artery (0.01% and in left anterior descending coronary artery (0.2%. The location of the lesion and the pathological condition, length and size of the coronary may dictate exposure of an intracavitary coronary artery for proper revascularization; more commonly surgeons are anaware of the intracavitary position and during intramyocardial dissection of an artery will open a cardiac chamber where the vessel traverses the cavity. Problems that arise are introducion

  10. Biblioteca universitária proativa

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    Maria de Fátima Pereira Raposo

    2006-07-01

    Full Text Available O trabalho visa apresentar alguns procedimentos que podem caracterizar uma postura proativa em bibliotecas universitárias. Para tanto, apresenta aspectos do conceito de proatividade em um contexto onde o uso e a aplicação das tecnologias de informação (TI constituem-se ferramentas essenciais para promover a inclusão social/digital no Brasil. O trabalho é abordado no contexto das bibliotecas do Centro Universitário da Cidade do Rio de Janeiro – UNIVER CIDADE. A adoção de procedimentos pró-ativos no Sistema de Bibliotecas da UNIVER CIDADE possibilitou um maior alcance dos problemas de informação de seus usuários, oferecendo, assim, subsídios para uma gestão eficiente da informação. Conseqüentemente, a confiança nos serviços da biblioteca e a participação nos programas instituídos cresceram proporcionalmente às ações pró-ativas adotadas pelo Sistema de Bibliotecas.

  11. Outras verdades, muito extraordinárias

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    Waldecy Tenório

    2006-12-01

    Full Text Available QUEM É A personagem enigmática a quem se dirige Riobaldo? Partindo de conceitos desenvolvidos pelo teólogo G. Crespy, por lingüistas como Julia Kristeva, A. J. Greimas e Roman Jakobson, assim como pelo crítico Wolfgang Iser, o autor deste ensaio percorre as páginas de Grande sertão: veredas para descobrir "outras verdades, muito extraordinárias": "Nonada" é uma invocação ou uma prece e a personagem enigmática do romance é Deus, a quem Riobaldo narra toda a sua vida, transformando a narrativa numa confissão geral. "Senhor o que acha?".WHO IS THE enigmatic character to whom Riobaldo speaks? Starting from the concepts developed by the theologian G. Crespy, by linguists such as Julia Kristeva, A. J. Greimas and Roman Jakobson, as well as by the critic Wolfgang Iser, the author goes through the pages of Grande sertão: veredas to discover "other, very extraordinary, truths": "Nonada" is an entreaty or prayer and the novel's enigmatic character is God, to whom Riobaldo narrates all his life, transforming the narrative in a general confession. "Sir, what do you think?"* * In Portuguese, the polite form that shows respect "Senhor" is used to address both: a man who is older, richer or more powerful and God. So, the same sentence could be translated in English to: "Lord, what do thou think?".

  12. Meningeoma da goteira olfatória

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    Tella Jr Osvaldo Inácio de

    2006-01-01

    Full Text Available Meningeomas da goteira olfatória representam 8-18% dos meningeomas intracranianos e geralmente são diagnosticados quando alcançam grande tamanho. Relatamos uma série de 13 casos consecutivos de meningeomas de goteira olfativa operados nos Hospitais São Paulo (UNIFESP e Professor Edmundo Vasconcelos no período de 1995 a 2003, estudados retrospectivamente quanto ao quadro clínico, resultados cirúrgicos e complicações. Os pacientes foram submetidos a ressecção cirúrgica destes tumores pela via subfrontal, em 9 casos a ressecção foi completa incluindo dura-máter e osso infiltrado por tumor e em 4 a dura-máter foi somente coagulada. Um paciente morreu devido a infarto cerebral e três pacientes evoluíram com fístulas liquóricas. Não houve recorrência sintomática no período que variou de 11 meses a 8 anos. Com as técnicas microcirúrgicas atuais, estes tumores podem ser removidos com baixa morbidade.

  13. Massimo Bontempelli: trajetória intelectual

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    Arivane Augusta Chiarelotto

    2010-11-01

    Full Text Available http://dx.doi.org/10.5007/2175-7917.2010v15n2p239 O italiano Massimo Bontempelli [1878-1960] é um escritor que, ao longo da carreira, conquistou uma fama controversa. Classificado de intelectual a serviço do fascismo, sua abordagem no campo literário é de natureza contestadora em relação a tradição Oitocentista. Além disso, sua trajetória resulta polêmica porque demarca a associação às vanguardas italianas insurgentes nas primeiras décadas do século XX. Tomadas as vanguardas pela sua gênese conceitual, Bontempelli esteve ligado a dois movimentos de grande impacto no contexto italiano: o futurismo e o fascismo. Neste trabalho abordaremos a sua inserção política, analisando aspectos de sua participação no cenário italiano que envolve a adesão e, posteriormente, o rompimento com o partido fascista.

  14. Fábulas fabulosas: denúncia, história e memória

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    Maria Angélica de Oliveira

    2016-01-01

    Full Text Available mo “Pelas mãos” do sujeito-autor Millôr Fernandes, as fábulas clássicas transitam por outros gêneros, são parodiadas, carnavalizadas, postas às avessas, transfigurando-se em fábulas fabulosas, um “outro gênero” em que a ironia, a sátira e a paródia fazem sítio. Estas estratégias irreverentes do dizer abrigam, nesses enunciados, um universo de vozes sociais que só se deixam entrever no diálogo com as condições de produção, com o contexto sócio-histó- rico-ideológico. Com o auxílio dos pressupostos teóricos da Análise de Discurso de linha francesa, procuramos identificar nessas fábulas fabulosas cicatrizes denunciadoras de sua identidade de pequenos repositórios de sabedoria que transitam entre a moral e a denúncia, entre as técnicas de si e as estratégias de resistência. Nossa pesquisa visa, ainda, evidenciar, nessas irreverentes e satíricas historietas, jogos de verdade, que materializados a cada novo acontecimento, na atualização da memória discursiva, domínios do intradiscurso, transformam-nas em argutas denúncias da história brasileira, conduzindo-as, pois, do gênero fá- bula fabulosa ao gênero monumento histórico. Palavras-chave: memória, técnicas de si, estratégias de resistência

  15. Memórias de Sefarad: o marrano como performance identitária na contemporaneidade

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    Vívien Gonzaga e Silva

    2011-10-01

    Full Text Available A partir da leitura de poemas que compõem o livro Memórias de Sefarad, de Leonor Scliar-Cabral, no qual se evidenciam vários traços da tradição sefardita, pretende-se abordar o fenômeno do “marranismo”. No século 15, ao mesmo tempo em que se instituía a designação de “cristão-novo” para o judeu convertido por ordem dos reis de Espanha, o rótulo “marrano” ‒ implicando aquele que simulava a conversão ao catolicismo enquanto mantinha a prática clandestina da fé judaica ‒ parece infiltrar-se no imaginário lentamente construído em torno do judeu de origem ibérica. Assim, o que poderia ficar circunscrito a um episódio da história do povo judeu, parece migrar no tempo e no espaço, acompanhando-o na diáspora, da sombra dos tribunais da Inquisição até os nossos dias. Acredita-se que a literatura pode constituir um espaço de problematização do marranismo como performance identitária, à medida que, no espaço da linguagem, como se verá na poesia de Scliar-Cabral, permite estabelecer um diálogo entre presente e passado como estratégia de resistência.

  16. Terapia Comunitária e Resiliência: histórias de mulheres

    OpenAIRE

    Braga,Lucineide Alves Vieira

    2009-01-01

    Muitas de nós, mulheres, resistimos firmes, apesar de muitos sofrimentos ao longo da vida. É essa superação que denominamos de resiliência, ou seja, a capacidade humana de enfrentar situações difíceis e sair delas, fortalecendo-se e transformando a realidade em que se vive. Através deste estudo, objetivamos conhecer as experiências resilientes das mulheres que frequentam as rodas de Terapia Comunitária Integrativa Sistêmica (TC), identificar as fontes de forças que elas utiliza...

  17. Histórias (co movedoras: História oral e estudos de migração

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    Thomson Alistair

    2002-01-01

    Full Text Available Este artigo pretende rever a contribuição dada pela história oral aos estudos sobre migração nos últimos vinte e cinco anos, especialmente entre a Grã-Bretanha e a Austrália. O título "histórias (co movedoras" é um útil jogo de palavras sobre a história oral da migração porque está centrado na experiência pessoal de mudança entre lugares, assim como indica a natureza comovedora dos relatos para o narrador e sua audiência.

  18. As estações viárias lusitanas nas fontes itinerárias da Antiguidade

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    Vasco Gil Mantas

    2014-12-01

    Full Text Available As fontes itinerárias essenciais do estudo das vias romanas da Lusitânia, Itinerário de Antonino, Cosmografia do Anónimo de Ravena e Itinerário de Barro de Astorga indicam um total de 56 estações viárias, 27 das quais ainda não identificadas com segurança. O presente artigo aborda os diferentes problemas que ainda subsistem e as suas causas e procura actualizar o quadro da geografia viária lusitana. 

  19. Vozes da memória : o contador de histórias em narrativas orais urbanas

    OpenAIRE

    Alessandra Bittencourt Flach

    2013-01-01

    Contar histórias é uma prática que atravessa os tempos e faz parte da natureza humana. Portanto, a área de Letras deve fazer desse tema objeto de estudo, com enfoque especial nas narrativas orais. Esta tese se propõe a discutir o papel do contador de histórias na pós-modernidade. Para tanto, serão analisados registros audiovisuais de um morador do bairro Restinga, em Porto Alegre (RS), com o intuito de demonstrar que ainda há espaço para ouvir e contar histórias, desde que isso seja pensado a...

  20. Literatura, história e memória : uma leitura da poesia de Mario Benedetti

    OpenAIRE

    Corrêa, Maria de Nazaré Fonseca

    2013-01-01

    Este trabalho tem como objetivo estudar o entrelaçamento de literatura, história e memória na poesia de Mario Benedetti, cujos temas abordam os fatos sociais e políticos acontecidos no Uruguai durante o período de ditadura (1973-1985). A realidade dos abusos e excessos cometidos nesses anos se manifesta nos poemas como uma forma de resistência e enfrentamento da dor. Na poesia de Benedetti, a memória assume o papel fundamental d...

  1. Advanced Beam-Dynamics Simulation Tools for RIA

    CERN Document Server

    Garnett, Robert; Crandall, Kenneth; Ostroumov, Peter; Qiang, Ji; Ryne, Robert D; Wangler, Thomas; York, Richard; Zhao, Qiang

    2005-01-01

    Understanding beam losses is important for the high-intensity RIA driver linac. Small fractional beam losses can produce radioactivation of the beamline components that can prevent or hinder hands-on maintenance, reducing facility availability. Operational and alignment errors in the RIA driver linac can lead to beam losses caused by irreversible beam-emittance growth and halo formation. We are developing multiparticle beam-dynamics simulation codes for RIA driver-linac simulations extending from the low-energy beam transport (LEBT) line to the end of the linac. These codes run on the NERSC parallel supercomputing platforms at LBNL, which allow us to run simulations with large numbers of macroparticles for the beam-loss calculations. The codes have the physics capabilities needed for RIA, including transport and acceleration of multiple-charge-state beams, and beam-line elements such as high-voltage platforms within the linac, interdigital accelerating structures, charge-stripper foils, and capabilities for h...

  2. Reabsorção dentária

    OpenAIRE

    Koval, Anna

    2015-01-01

    Projeto de Pós-Graduação/Dissertação apresentado à Universidade Fernando Pessoa como parte dos requisitos para obtenção do grau de Mestre em Medicina Dentária Este trabalho científico no âmbito de conclusão do curso de Mestrado Integrado em Medicina Dentária na Universidade Fernando Pessoa teve como principal objetivo, a realização de uma revisão bibliográfica acerca de reabsorções dentárias. Os objetivos particulares foram: classificar na sua generalidade as reabsorções dentárias, analisa...

  3. Epistemologia pluralizada e história da psicologia

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    José Antônio Damásio Abib

    2009-06-01

    Full Text Available Tomam-se, aqui, obras de Wilhelm Wundt e William James para mostrar que a psicologia nasce como projeto científico no final do século XIX. Esse projeto diferencia psicologia como ciência de psicologia como metafísica. Dessa perspectiva, a história da psicologia é concebida como história da psicologia científica e a pré-história da psicologia é concebida como história da psicologia metafísica. Mas as concepções de ciência de Wundt e James são diferentes. Da perspectiva da epistemologia unitária, a psicologia não se constitui como ciência. Nesse caso, a história da psicologia é concebida como pré-história dessa disciplina e sua história como ciência só começará se ela adquirir unidade. Da perspectiva da epistemologia pluralizada, a história da psicologia é história da cultura, o que significa dizer que é história das tradições de pensamento psicológico e filosófico e, também, história das ideias. Conclui-se que, da perspectiva da epistemologia pluralizada, epistemologia e história da psicologia adquirem uma perspectiva antropológica, o que equivale a dizer que a pré-história da psicologia não existe, e que a história dessa disciplina pode começar em qualquer época e lugar. E, finalmente, que o esclarecimento da psicologia como ciência depende da história da ciência e da cultura psicológica.Wilhelm Wundt and William James opus are used here to argue that psychology emerges as scientific project in the end of nineteenth-century. Psychology as science and as metaphysics is distinguished by this project. From this point of view, history of psychology is to be conceived as history of scientific psychology and prehistory of psychology is to be conceived as history of metaphysical psychology. But Wundt and James conceptions of sciences are different. From the unitary epistemological view, psychology is not constituted as science. In this case, history of psychology is to be conceived as prehistory of

  4. Integrating e-Payment Services with RIA Applications

    Directory of Open Access Journals (Sweden)

    Mihai Pricope

    2010-12-01

    Full Text Available Given the expansion of RIA technologies, it is highly important to deliver a set of best practices for developers in order to integrate e-business applications with e-payment systems. In this article we present a solution for the integration of PayPal Express with a RIA application developed on Flex. We focus both on security and functionality issues.

  5. Uma teoria do abuso da História

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    Antoon De Baets

    2013-01-01

    Full Text Available Este ensaio pretende esboçar uma teoria coerente sobre o abuso e o uso irresponsável da história. Ainda não existe de forma acabada uma teoria como essa, com a qual historiadores possam identificar, provar, explicar e avaliar abusos da história. O texto começa com uma discussão sobre a delimitação do problema, ou seja, como distinguir uma história irresponsável e abusiva, de um lado, de uma história perigosa, não científica, incompetente, nociva e desprovida de sentido, de outro. Em seguida, define-se o abuso da história como a sua utilização com o intuito de ludibriar, e o conceito mais amplo de uso irresponsável da história como o seu uso negligente ou enganador. Finalmente, vários desdobramentos da teoria serão desenvolvidos, desde uma tipologia dos abusos e dos usos irresponsáveis, questões relativas a sua evidência, explicação e avaliação, até medidas para sua prevenção.

  6. MISSIONÁRIAS PROTESTANTES AMERICANAS (1870 – 1920: GÊNERO, CULTURA, HISTÓRIA

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    Eliane Moura da Silva

    2011-01-01

    Full Text Available Esse artigo trata as relações entre religião, cultura e papéis de gênero entre 1870 e 1920 tendo como objeto de estudo as missionárias protestantes americanas durante colonialismo e imperialismo. Trabalhando com a perspectiva das relações entre gênero, cultura e religião têm como objetivo apresentar algumas reflexões teóricas sobre a história cultural das religiões e de gênero. Parte-se do pressuposto analítico de que as missões cristãs em geral, e as protestantes em particular, foram mais do que colonizações culturais: a mesma missão que converteu o Outro trouxe o Outro para dentro de si e construíram novas culturas e fronteiras e relações entre gênero e religião.

  7. Esquizofrenia refratária Refractory schizophrenia

    Directory of Open Access Journals (Sweden)

    Helio Elkis

    2007-10-01

    Full Text Available OBJETIVO: O propósito deste artigo é o de revisar vários aspectos da esquizofrenia refratária levando em conta questões relacionadas à definição, aspectos clínicos, correlatos psicobiológicos, tratamentos farmacológicos e não farmacológicos, assim como preditores de resposta terapêutica. MÉTODO: Pesquisa no Medline, assim como artigos dos autores. RESULTADOS E CONCLUSÕES: Pelo menos um terço dos pacientes com esquizofrenia são refratários a tratamento com antipsicóticos e as evidências apontam a clozapina em monoterapia como a principal opção nesses casos. A politerapia com antipsicóticos não tem apoio em evidências. Ensaios clínicos recentes mostraram que a potencialização da clozapina com outros antipsicóticos não é superior ao placebo.OBJECTIVE: The aim of the present paper is to review the various aspects of refractory schizophrenia regarding issues such as definitions, clinical aspects, psychobiological correlates, pharmacological and non-pharmacological treatment options and predictors of treatment response. METHOD: Medline search as well as articles of the authors. RESULTS AND CONCLUSIONS: Refractory schizophrenia affects at least one third of patients with schizophrenia and the best evidence shows that is monotherapy with clozapine remains the mainstay for the treatment of such condition. Antipsychotic polipharmacy is not supported by current evidence and recent clinical trials have shown that clozapine augmentation with antipsychotics has no benefit over placebo.

  8. Reatividade vascular da artéria mamária interna: estudos farmacológicos comparativos entre artérias caninas direita e esquerda

    OpenAIRE

    1999-01-01

    Para estudos comparativos da reatividade vascular entre artéria mamária interna (AMI) canina direita e esquerda, realizaram-se experimentos "in vitro" utilizando-se banhos orgânicos ("organ chambers") e ensaios biológicos: 1) os produtos plaquetários ADP e 5-HT induziram, respectivamente, vasodilatação dependente e independente do endotélio; 2) os autacóides, bradicinina e histamina, também induziram vasodilatação, respectivamente, dependente e independente do endotélio; 3) o A23187, vasodila...

  9. Production of specific antisera for radioimmunoassay of human luteinizing hormone (LH) in the presence of human chorionic gonadotropin (hCG). [/sup 125/I

    Energy Technology Data Exchange (ETDEWEB)

    Thorell, J.I.; Jeppsson, S.; Holmstrom, B.

    1976-09-01

    A specific radioimmunoassay for LH, which measures plasma LH in the presence of human chorionic gonadotropin (hCG) is described. Rabbits were immunized with highly purified native LH. One of the antisera with a difference in its reactivity against LH and hCG was further purified by affinity chromatography on a column with hCG coupled to Sepharose 4B. The adsorbed antiserum and /sup 125/I-LH was used in a double antibody assay. The LH standard (MRC/68/40) efficiently inhibited the binding of /sup 125/I-LH, and the standard curve showed a sensitivity of 0.5 ng/ml in the sample. hCG up to 10,000 ng/ml did not inhibit the binding of /sup 125/I-LH. The plasma level of LH in pregnant women in the first trimester was low (1.3 +- 0.1 ng/ml). When LH was measured in fertile or menopausal women with or without stimulation with LH/FSH releasing hormone (LH-RH)/sup x/ the results agreed to those found with our conventional LH-assay based on antiserum against hCG.

  10. A comparative study of the peroxidase-antiperoxidase method and an avidin-biotin complex method for studying polypeptide hormones with radioimmunoassay antibodies.

    Science.gov (United States)

    Hsu, S M; Raine, L; Fanger, H

    1981-05-01

    A highly sensitive immunoenzymatic technic is presented. The method involves three sequential steps: (1) primary antibody, (2) biotin-labeled secondary antibody, and (3) avidin-biotin-peroxidase complex. Avidin, an egg white protein, has four binding sites for the low-molecular-weight vitamin biotin. Many moieties of biotin can be coupled to the peroxidase molecule. Thus, since a relatively large amount of avidin is incubated with biotin-labeled peroxidase, avidin serves as a link between biotin-peroxidase molecules; in turn, biotin-peroxidase serves as a link between avidin molecules. Consequently, this large lattice-like complex with biotin-binding capability can be attracted to the sites of biotin-labeled antibody, producing a superior staining sensitivity. Several commercially available radioimmunoassay antibodies (e.g., antiglucagon, prolactin, gastrin, growth hormone, and thyroid-stimulating hormone antibodies) were tested for immunohistochemical staining. The unlabeled antibody peroxidase-antiperoxidase method fails to stain gastrin or thyroid-stimulating secretory cells when using these antibodies, and a relatively high antibody concentration is required to produce a positive reaction for glucagon, prolactin, and growth hormone. In contrast, the avidin-biotin-peroxidase complex method successfully demonstrates polypeptide hormones even when antibodies are diluted 20 to 40 times.

  11. Radioimmunoassay for guanosin-5'-diphosphate-3'-diphosphate and adenosine-5'-triphosphate-3'-diphosphate

    Energy Technology Data Exchange (ETDEWEB)

    Hamagishi, Y.; Oki, T.; Tone, H.; Inui, T. (Sanraku-Ocean Co. Ltd., Fujisawa, Kanagawa (Japan). Central Research Lab.)

    1980-12-01

    A radioimmunoassay for guanosine-5'-diphosphate-3'-diphosphate (ppGpp) and adenosine-5'-triphosphate-3'-diphosphate (pppApp) has been developed. The assay method is based on competition of an unlabeled highly phosphorylated nucleotide with /sup 3/H-labeled highly phosphorylated nucleotide for binding sites on a specific antibody. Antibodies to ppGpp and pppApp were obtained by immunizing rabbits with the antigen prepared by conjugating ppGpp with human serum albumin using 1-ethyl-3-(3-dimethylaminoprophyl)carbodiimide, and with the antigen prepared by conjugating 8-(6-aminohexyl)amino-adenosine-5'-triphosphate-3'-diphosphate with human serum albumin using glutaraldehyde, respectively. Antibody-bound /sup 3/H-labeled highly phosphorylated nucleotides were separated from the free /sup 3/H-labeled highly phosphorylated nucleotides by selective adsorption on dextran-coated charcoal. Displacement plots were linear over a concentration range of 5 - 1,000 pmol/assay tube in a log-probit percentage plot. Application of this method to biological systems offers improved accuracy and convenience compared with the previous /sup 32/PO/sub 4/-labeling technique.

  12. Synthesis of N,N,-didemethylzolpidem-N-{l_brace}2-{l_brace}3-(4-hydroxy-3-[{sup 125}I] iodophenyl){r_brace}methyl propionate{r_brace} for radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    De Clerck, I.; Daenens, P. [K.U. Leuven (Belgium). Laboratory of Toxicology

    1997-03-01

    The {sup 125}I-derivative of zolpidem was prepared by iodination of a L-tyrosine methyl ester conjugate of a zolpidem precursor. The iodinated compound has been used as a tracer molecule in the development of a radioimmunoassay for zolpidem. It was purified by normal phase HPLC, in combination with gamma counting detection. The structure was confirmed by high resolution L-SIMS. (UK).

  13. Síndromes autoinflamatórias hereditárias na faixa etária pediátrica Pediatric hereditary autoinflammatory syndromes

    Directory of Open Access Journals (Sweden)

    Adriana Almeida Jesus

    2010-10-01

    Full Text Available OBJETIVO: Descrever as principais síndromes autoinflamatórias hereditárias na faixa etária pediátrica. FONTES DOS DADOS: Foi realizada uma revisão da literatura nas bases de dados PubMed e SciELO, utilizando as palavras-chave "síndromes autoinflamatórias” e "criança”, e incluindo referências bibliográficas relevantes. SÍNTESE DOS DADOS: As principais síndromes autoinflamatórias são causadas por defeitos monogênicos em proteínas da imunidade inata, sendo consideradas imunodeficiências primárias. Elas são caracterizadas clinicamente por sintomas inflamatórios sistêmicos recorrentes ou contínuos e devem ser diferenciadas das doenças infecciosas, autoimunes e outras imunodeficiências primárias. Nesta revisão, foram enfatizadas características epidemiológicas, manifestações clínicas, alterações laboratoriais, prognóstico e terapia das principais síndromes autoinflamatórias: febre familiar do Mediterrâneo; síndrome periódica associada ao receptor de fator de necrose tumoral; criopirinopatias; deficiência de mevalonato-quinase; artrite granulomatosa pediátrica; síndrome de pioderma gangrenoso, artrite piogênica e acne; síndrome de Majeed; e deficiência do antagonista do receptor de interleucina-1. As criopirinopatias discutidas foram: doença inflamatória multissistêmica de início neonatal ou síndrome neurológica, cutânea e articular crônica infantil, síndrome de Muckle-Wells e síndrome autoinflamatória familiar associada ao frio. CONCLUSÕES: É importante que o pediatra reconheça as síndromes autoinflamatórias hereditárias mais prevalentes, pois o encaminhamento ao reumatologista pediátrico pode permitir um diagnóstico precoce e uma instituição de tratamento adequado, possibilitando uma melhora da qualidade de vida dos pacientes.OBJECTIVE: To describe the most prevalent pediatric hereditary autoinflammatory syndromes. SOURCES: A review of the literature including relevant references

  14. História, memória e esquecimento: Implicações políticas

    Directory of Open Access Journals (Sweden)

    Maria Paula Nascimento Araújo

    2012-10-01

    Full Text Available Pesquisadores têm colocado em questão a recuperação de situações traumáticas como as que ocorreram no Holocausto, no bombardeio a Hiroshima, na guerra do Vietnam ou nos massacres fratricidas da Iugoslávia. Embora algumas contribuições clássicas tenham assinalado aspectos importantes relativos à história e memória, há várias formas de lidar com o passado e todas elas envolvem interesse, poder e exclusões. A política da justa memória a ser realizada sobre crimes cometidos passados, debate que vem sendo travado não só em diversas áreas acadêmicas, como na sociedade em geral, depende de processos seletivos, bem como de elementos que excedem o escopo da razão humana. É preciso encontrar o equilíbrio entre a obsessão pelo passado e as tentativas de imposição do esquecimento. Nosso intuito, portanto, é o expandir o conhecimento sobre história, memória e esquecimento, ressaltando limites, bem como implicações éticas e morais.

  15. Histórias de aprendiz: memórias, narrativas e formação docente

    Directory of Open Access Journals (Sweden)

    Lúcia Gracia Ferreira

    2015-12-01

    Full Text Available Este estudo tem como objetivo relatar histórias de aprendiz produzidas no âmbito do curso de extensão “Formação de professores: um desafio da contemporaneidade”, desenvolvido na Universidade Estadual do Sudoeste da Bahia, Campus de Itapetinga, e os aspectos formativos presentes nas histórias. A discussão do curso incidia sobre a formação de professores na contemporaneidade e os encontros eram realizados semanalmente. Assim, este trabalho buscou identificar nas histórias de aprendiz narradas, memórias e nessas as experiências formativas nela presente. Para alguns eram apenas histórias, para outros lembranças, aqui mostro que é mais que isso, é formação. A partir da análise das narrativas foi possível perceber como sujeitos mudaram, formaram, viveram. Assim, considero as histórias de aprendiz, narrativas de fragmentos da vida, que possibilita reflexão, que possibilita a autoformação.

  16. Por que aprender História? Why learn History?

    Directory of Open Access Journals (Sweden)

    Peter Lee

    2011-12-01

    Full Text Available A partir da constatação de que ninguém escapa do passado e da importância da relação que as pessoas estabelecem com o passado, buscou-se apontar, a partir de pesquisa em fontes relacionadas à filosofia da História, alguns fundamentos para o significado da aprendizagem da história. Nesse sentido, temas como a importância da História para a validação do passado, a relevância das evidências, a problemática das leis e generalizações, bem como o significado da experiência vicária para a construção da aprendizagem histórica, foram objetos de análise, no sentido de se mostrar por que é importante aprender História.Knowing that nobody escapes from the past and the importance of the relationship that people establish with the past, this aimed to point out some fundaments for the meaning of History learning, based in researches related to History's philosophy. Thus, themes such as the importance of History to validate the past, the relevance of evidences, the problematic of laws and generalizations and also the meaning of vicarial experience to build the learning of History were objects of analysis with the objective of showing why it is important to learn History.

  17. Hydrodynamic Aspects at Vitória Bay Mouth, ES

    Directory of Open Access Journals (Sweden)

    FLÁVIA A.A. GARONCE

    2014-06-01

    Full Text Available Understading the hydrodynamic behavior and suspended particulated matter (SPM transport are of great importance in port regions such as Vitória Harbor, which is located at Vitória Bay, Vitória – ES, Brazil. Vitória Bay is an estuary that has not been systematically assessed through a temporal analysis in order to identify its hydrodynamics characteristics and SPM exchange. This study aims to investigate salt and suspended particulate matter flux at the estuarine mouth of Vitória Bay by understanding the temporal variation of salinity, temperature and tidal currents within the water column and at the channel crosssection. Results showed that the estuarine mouth tended to present partial stratification periods during neap tides and little stratification in spring tides. The circulation pattern was mainly influenced by the tide, with little influence from river discharge. With regard to the SPM, the mouth of the estuary tended to show low concentrations, with the highest values occurring during the dry season. A close relationship between momentary discharge, SPM and salt fluxes was observed. Despite all the data was collected at the mouth of the estuary, the system showed an importation trend of salt in all cycles and SPM importation for three of the four studied tidal cycles. Thus, Vitoria Bay is not exporting SPM to the adjacent inner shelf.

  18. De ontwikkeling van een radioimmunologische bepalingsmethodiek (RIA) voor oestronsulfaat in bloedplasma ten behoeve van een drachtigheidstest bij het varken = The development of radio-immuno-assay (RIA) for oestrone sulphate in bloodplasma for the purpose of pregnancy - diagnosis in the pig

    NARCIS (Netherlands)

    Vos, E.A.; Wiel, van de D.F.M.

    1984-01-01

    Naast een beschrijving van de ontwikkeling van de techniek zijn de resultaten van een test opgenomen. De gevonden verschillen in concentraties van het hormoon oestronsulfaat bieden mogelijkheden om een drachtigheidstest te ontwikkelen

  19. Serum 3,3'-L-diiodothyronine, a direct radioimmunoassay in human serum: method and clinical results. [/sup 125/I tracer technique

    Energy Technology Data Exchange (ETDEWEB)

    Burger, A.; Sakoloff, C.

    1977-09-01

    A specific radioimmunoassay for 3,3'-diiodothyronine (T/sub 2/) is described which is capable of detecting as little as 1.3 ng/dl. The antiserum recognizes mainly T/sub 2/; biliary conjugates of T/sub 2/ bind slightly to the antibody. The intraassay and interassay coefficients of variation were, respectively, 5.7% and 13.1%. T/sub 2/ was detected in the serum of hypothyroid patients treated with triiodothyronine (T/sub 3/) and in euthyroid subjects treated with reverse triiodothyronine (rT/sub 3/). These results suggest that both T/sub 3/ and rT/sub 3/ are deiodinated to T/sub 2/. Serum concentrations of T/sub 2/ in normal subjects decreased with age. Between 20 and 40 years the mean concentration was 4.3 +- 2.0 ng/dl (2 SD), between 41 and 60 years it varied from 1.9 to 5.8 ng/dl (3.8 +- 0.3 ng/dl, SE) and in elderly subjects have 60 years concentrations varied from unmeasurable to 4 ng/dl (2.9 +- 0.4 ng/dl, SE). Low serum T/sub 2/ concentrations were found in anorexia nervosa (2.5 +- 0.3 ng/dl, SE). In hypothyroidism the serum concentrations were low or unmeasurable. As most of the hypothyroid subjects were elderly their serum T/sub 2/ concentrations overlapped with the low values found in the elderly euthyroid subjects. In classical hyperthyroidism serum T/sub 2/ concentrations were greatly increased (3.3 to 31 ng/dl (11.8 +- 2.7 ng/dl, SE) but in ''T/sub 3/ toxicosis'' the concentrations were only modestly increased (2.4 to 8.8 ng/dl, 5.2 +- 0.8 ng/dl, SE).

  20. Radioimmunoassay for 3,3',5'-triiodo-L-thyronine in unextracted serum: method and clinical results. [/sup 125/I

    Energy Technology Data Exchange (ETDEWEB)

    Nicod, P.; Burger, A.; Staeheli, V.; Vallotton, M.B.

    1976-05-01

    Serum 3,3',5'-triiodothyronine (rT/sub 3/) was measured with a radioimmunoassay in unextracted serum. The assay was specific and reproducible. The coefficients of variation for three different sera known for high, normal, and low rT/sub 3/ concentrations between assays were 4, 6, and 9 percent and within assays 4, 9, and 7 percent, respectively. In euthyroid subjects 20 to 60 years old, rT/sub 3/ was 450 +- 200 pg/ml (mean +- SD, n = 83). Serum rT/sub 3/ was found to be increased in hyperthyroidism (range: 762 to 2581 pg/ml; n = 11) but also in acute and chronic illness (up to 2400 pg/ml; n = 24) and in anorexia nervosa (536 to 1058 pg/ml; n = 7). In the latter two situations there was mostly an inverse change in serum 3,5,3'-triiodothyronine (T/sub 3/) which was in the low normal range or decreased. These findings suggest a metabolic control of thryoxine deiodination. A low serum rT/sub 3/ was found in 9 of 12 hypothyroid patients and in the serum of one chronically ill patient. Long-term treatment (1 to 7 years) with lithium carbonate slightly reduced serum rT/sub 3/, although the changes were inside the normal range. Kidney function was not found to be necessary for its production, as anephric patients had normal rT/sub 3/ values. In addition, hemodialysis increased serum rT/sub 3/, which is probably due to the heparin therapy.

  1. Barbas, Helena, Madalena. História e Mito

    Directory of Open Access Journals (Sweden)

    Sidinea Pedreira

    2012-10-01

    Full Text Available É inquestionável a importância da recuperação e reconfiguração de histórias de personagens que contribuíram, ao longo dos séculos, para a criação e manutenção de estereótipos que serviram para a legitimação de papéis sociais e de comportamentos. Reescritas e reinterpretações da história – seja de relatos históricos, seja de mitos ou lendas – são possíveis e necessárias, uma vez que proporcionam espaços de discussão e de reconfiguração de papéis sociais, nomeadamente no que diz respeito às ide...

  2. REMESSA NECESSÁRIA NO DIREITO DO TRABALHO

    Directory of Open Access Journals (Sweden)

    Silvana Maria de Oliveira Prince Rodrigues

    2012-08-01

    Full Text Available RESUMOO presente estudo tem por objetivo analisar o instituto da remessa necessária em face das garantias constitucionais da isonomia e da efetividade processual. Discute-se a questão da possibilidade da reformatio in pejus e suas consequências e a aplicabilidade da remessa necessária no Direito do Trabalho.Palavras- chave: Remessa Necessária; Isonomia; Efetividade; Direito do Trabalho. MANDATORY REVIEW IN LABOR LAW ABSTRACTThe objective of this study is to analyze the mandatory review examined in light of the constitutional guarantees of equality and effectiveness of judicial decisions. The study discusses the possibility of reformation in pejus and its consequences, and the applicability of mandatory review in Labor Law.Keywords: Mandatory Review; Equality; Effectiveness; Labor Law.

  3. Psicologia Comunitária: uma abordagem conceitual

    OpenAIRE

    Gomes, Antonio Maspoli de Araujo

    2009-01-01

    Este artigo apresenta as principais tentativas de conceituação das correntes teóricas sobre a psicologia comunitária, ligadas a psicologia social. A partir da análise dos dados obtidos podemos afirmar que a Psicologia Comunitária é um saber em construção, pragmático, derivado da Psicologia Social. Concluímos também que muitas das teorias referenciadas e das técnicas citadas pelos psicólogos que trabalham em Psicologia Comunitária são derivadas dos referenciais teóricos da Psicologia Comunitár...

  4. Kant sobre o progresso na história

    Directory of Open Access Journals (Sweden)

    Joel Thiago Klein

    2013-07-01

    Full Text Available http://dx.doi.org/10.5007/1677-2954.2013v12n1p67O objetivo deste artigo é analisar o significado da tese central da filosofia kantiana da história: que a humanidade se encontra em um constante progresso para o melhor. Contudo, apesar de parecer a primeira vista simples, a discussão do próprio significado dessa  tese está longe de alcançar um consenso na literatura. A originalidade deste trabalho é apresentar pela primeira vez um mapeamento geral, sistemático e exaustivo das diferentes interpretações e críticas que essa tese recebeu ao longo da história. Nesse sentido, qualquer resposta que pretenda ser minimamente satisfatória precisa levar em consideração todos os argumentos e posições aqui apresentados.

  5. A história das disciplinas escolares

    OpenAIRE

    Antonio Viñao

    2012-01-01

    A história das disciplinas escolares constitui um campo de investigação configurado a partir dos anos de 1970, sobretudo na Grã-Bretanha por Ivor F. Goodson, entre outros, com a influência da “nova sociologia da educação” inglesa e dos chamados “estudos do currículo”, e na França, também entre outros, por Dominique Julia e André Chervel. Nesse caso, no marco da também chamada “história cultural” e, no âmbito da educação, da história da “cultura escolar”. Esse campo seria introduzido e desenvo...

  6. Anatomia do sulco da artéria vertebral

    OpenAIRE

    CARVALHO, Max Franco de; Rocha,Roberta Teixeira; Monteiro,João Tiago Silva; Pereira, Carlos Umberto; Defino,Helton Luiz Aparecido

    2009-01-01

    INTRODUÇÃO: Diversas técnicas cirúrgicas têm sido realizadas na região craniocervical devido a diversas patologias. Durante o acesso cirúrgico a essa região existe um risco potencial de lesão iatrogênica da artéria vertebral, relacionado ao acesso lateral amplo e à avaliação inadequada da anatomia local. Variações no trajeto da artéria vertebral ocasionam maior risco de lesão vascular. O estudo pré-operatório por imagem da anatomia da artéria vertebral e do seu sulco tem sido realizado para a...

  7. Heavy-ion beams required for the RIA accelerator

    CERN Document Server

    Pardo, Richard C; Nolen, J A; Rehm, K E; Savard, Guy

    2004-01-01

    A class of experiments which will be representative of the expected initial fields of study at the Rare Isotope Accelerator Facility (RIA), are discussed. Improvement in the understanding of the rapid neutron capture process that is responsible for the creation of most stable nuclei heavier than the iron-region nuclei, will be the most important areas of research with RIA. RIA will provide beams of nuclei far from stability at low energies and with excellent beam quality, similar to the properties of stable beams available from facilities such as ATLAS. A total of 21 driver beams are identified which are necessary to provide optimal population of the r-process path, and provide good production of light neutron-rich nuclei. (Edited abstract) 15 Refs.

  8. A memória ventre da alma

    Directory of Open Access Journals (Sweden)

    Marina Massimi

    2010-12-01

    Full Text Available O artigo aborda o tema da memória como proposto num sermão de Vieira (1654, buscando reconstruir as matrizes conceituais (a concepção de memória de Agostinho e de Bernardo e práticas (a tradição da ortopraxis desenvolvida nas comunidades monásticas na Idade Média. Para ambos, a memória é concebida como processo ativo e abrangente, intimamente associada à emoção e à imaginação: não apenas rememora, mas organiza experiências e conceitos segundo um esquema norteado por lugares e percursos, tornando-os disponíveis para a invenção e a construção cognitiva.

  9. Os signos da memória em Milan Kundera

    OpenAIRE

    Silva, Rosimara Aparecida da

    2011-01-01

    A dissertação Os signos da memória em Milan Kundera pretende pensar de que forma as imagens apreendidas pela memória, quando traduzidas pela linguagem, viram signos carregados de significados no processo de criação de Milan Kundera. Para tanto, nos aprofundamos na teoria do filósofo Henri Bergson, que apresenta a memória como instância que estabelece a relação entre corpo e espírito e encontra na intuição o caminho para se atingir a interioridade do ser. Nossa hipótese é a de que Milan Kunder...

  10. As teorias da história e a história ensinada no ensino fundamental

    Directory of Open Access Journals (Sweden)

    Lia Machado Fiuza Fialho

    2017-02-01

    Full Text Available Resumo: Objetiva discutir as interfaces das teorias da História e a História ensinada no ensino fundamental, com ênfase na “alfabetização histórica” no século XXI. Como metodologia de pesquisa utilizou-se grupo focal, com 21 pesquisadores e professores que atuavam no campo do Ensino de História e História da Educação. Constatou-se que o ensino no século XXI deve propiciar a aprendizagem de maneira contextualizada, relacionando a pessoa, o tempo e o espaço, em uma perspectiva de produção e transformação da sociedade, na qual o aluno é concebido como sujeito histórico inserido no âmbito dialógico-crítico, tendo o professor como um mediador. Palavras-chave: Ensino. História. Correntes teóricas.

  11. Biblioteca universitária, escolar e comunitária: o caso da biblioteca comunitária “Professora Ebe Alves da Silva” do IFMG

    Directory of Open Access Journals (Sweden)

    Helena Maria Tarchi Crivellari

    2015-12-01

    Full Text Available A Expansão da Rede Federal de Educação Profissional, Científica e Tecnológica ocorrida em 2008, pela lei 11.892, transformou diferentes instituições de ensino técnico federais em Institutos Federais de Educação, Ciência e Tecnologia, que são responsáveis pela educação profissional em diferentes modalidades de ensino, promovendo tanto a educação básica quanto a educação superior. Inseridas nesse contexto estão as bibliotecas dessas instituições, que são, concomitantemente, bibliotecas escolares e universitárias. Com o objetivo de demonstrar o papel dessas bibliotecas, motivado pelo questionamento sobre a possibilidade de convivência desses tipos de bibliotecas em um mesmo espaço, apresenta-se um estudo de caso sobre a Biblioteca Comunitária Professora Ebe Alves da Silva, do Instituto Federal de Minas Gerais que, além de escolar e universitária é também “comunitária”, pela sua própria denominação. Para a concretização do estudo de caso foram realizadas entrevistas e aplicados questionários, dirigidos a diferentes atores da comunidade interna e externa da instituição. Os resultados foram submetidos à técnica do Discurso do Sujeito Coletivo – DSC - que ilustrou a discussão, relacionando os aspectos teóricos sobre biblioteca escolar, biblioteca universitária e biblioteca comunitária à realidade da Biblioteca Comunitária Professora Ebe Alves da Silva. A pesquisa concluiu que a biblioteca estudada cumpre os três papéis, constituindo-se em uma agente propulsora da democratização da informação e contribuindo para o desenvolvimento do município.

  12. Economia solidária versus economia capitalista

    Directory of Open Access Journals (Sweden)

    Paul Singer

    2001-12-01

    Full Text Available A economia solidária para ser competitiva no mercado capitalista não pode se furtar à concentração que incorpora progresso técnico. Experiências vêm demonstrando que cada associação ou cooperativa tem de atingir um tamanho em que ela possa resgatar seus membros da pobreza e acumular sobras que multipliquem fontes de trabalho e renda solidários. Para a economia solidária a conglomeração deve ser fácil porque a solidariedade torna natural a associação entre cooperativas.

  13. Precision Ibéria : an opportunity on Facebook

    OpenAIRE

    Gomes, Cátia Daniela Nunes

    2012-01-01

    Precision Ibéria is a franchise company owned by 100% Portuguese investors. Characterized by being a Multibrand auto repair company, it has been present in Portugal for 10 years; Precision Ibéria is positioned as “one stop shop” providing all the mechanical services for all passenger car brands. From its beginning it has been focusing on consumer satisfaction, developing strat-egies that meet their needs. Unfortunately, the crisis that affects our country has reached the repair aftermarket se...

  14. Aspectos estruturais da membrana eritrocitária

    OpenAIRE

    Murador, Priscila [UNESP; Deffune, Elenice

    2007-01-01

    Este artigo descreve as estruturas e funções da membrana eritrocitária e sua importância na medicina transfusional. A membrana eritrocitária é uma das membranas mais conhecidas em termos de estrutura, função e genética. Como qualquer membrana plasmática, tem como função mediar transportes e, ainda, fornece ao eritrócito resistência e maleabilidade. De acordo com a International Society of Blood Transfusion (ISBT), são mais de 500 antígenos expressos na membrana das hemácias e, destes, cerca d...

  15. Memória: entre o oral e o escrito

    Directory of Open Access Journals (Sweden)

    Maria Aparecida Bergamaschi

    2012-07-01

    Full Text Available  O trabalho discute a relação entre o oral e o escrito sob a perspectiva da memória como experiência humana. Retoma as significações de memória que prevaleceram na história do ocidente, relacionando-as com a oralidade, a escrita e as práticas escolares, evidenciando que, na medida em que a presença da escrita vai se tomando cada vez mais intensa na sociedade e na escola, a memória vai perdendo o prestígio e aparece de forma mais contundente a dicotomia entre memória oral e escrita. O conceito de apropriação, desenvolvido por Chartier, é utilizado para reatar a relação entre memória oral e escrita tendo como palco de análise as práticas escolares e as práticas de escrita entre alguns grupos indígenas brasileiros que têm, historicamente, a tradição oral como traço fundante de suas culturas e que, ao fazerem uso da escrita, reelaboram essa linguagem através de novas relações com a memória.Palavras-chave: oralidade e escrita, memória, práticas escolares.   Abstract The paper discusses the relation between the oral and the written under the perspective of memory as a human experience. It retakes the meanings of memory that prevailed in western history, relating it with the orality, the writing and the school practices, proving that, as the presence of writing becomes more intense in society and in school, memory looses prestige and the dichotomy appears in a stronger way between written and oral memory. The concept of appropriation developed by Chartier, is used in order to tie again the relation between oral and written memory by analyzing school practices and writing practices among some Brazilian indian groups. These groups have, historically, the oral tradition as foundation of their culture and, when writing, they reelaborate this language through new relations with memory. Keywords: oral and written, memory, school practices. 

  16. Interfaces entre psicologia social comunitária e psicodrama

    OpenAIRE

    2009-01-01

    O presente artigo apresenta a relação entre psicologia social comunitária e psicodrama. Examinaram-se os conceitos e visões de homem e de mundo, o compromisso social e as dimensões metodológicas de ambos os saberes, para sistematizar os elementos em comum encontrados. Dentre as interfaces de maior relevância encontradas, pode-se citar que a psicologia comunitária e o psicodrama construíram-se de forma pragmática; compreendem o homem como ser sócio-histórico e relacional; privilegiam o trabalh...

  17. A história e a temporalidade do risco.

    Directory of Open Access Journals (Sweden)

    Carlos Henrique Armani

    2015-06-01

    Full Text Available A partir de um enfoque centrado na história das ideias, pretendo apresentar algumas particularidades do risco como um modo de pensar as sociedades ocidentais contemporâneas. Ainda que o fenômeno do risco e as sociedades balizadas por sua temporalidade possam ser encontrados ao longo da história, entendo que é fundamental elaborar uma distinção entre os tempos do risco, de modo que possamos compreendê-lo em sua lógica temporal contemporânea.

  18. Memória e Pastiche em Leite Derramado

    Directory of Open Access Journals (Sweden)

    Mírian Sumica Carneiro Reis

    2016-07-01

    Full Text Available Neste artigo, propomos uma análise do romance Leite Derramado, de Chico Buarque, considerando que, em sua tessitura, a memória é tema e trabalho de citação, a partir dos quais ecoam as vozes de outros autores, textos, teorias, mas também da ruptura com a historiografia tradicional e da derrocada de valores e ideologias para as quais nem o aspecto de criação da memória pode representar redenção.

  19. Bibliografia: caminhos da história contada e da história vivida

    Directory of Open Access Journals (Sweden)

    Eduardo Alentejo

    2015-08-01

    Full Text Available Introdução: Trata sobre a Bibliografia tendo como referência sua historiografia. Tem por material as bibliografias citadas, os clássicos e os autores que marcaram o desenvolvimento da área.Objetivo: Visa analisar a bibliografia como sendo campo de interesse científico, buscando responder quais são os caminhos de sua história contada e de sua história vivida que a legitimam como campo de interesses científicos?Metodologia: A partir do método historiográfico, discorre sobre o estado atual do ensino brasileiro da Bibliografia. Mediante revisão de literatura, explica sua divisão em ramos e discorre sobre sua historiografia de acordo com contornos históricos dos quais envolvem aspectos tecnológicos da produção do conhecimento.Resultados: Explica que a polifonia e a vulgarização acerca de seu campo científico são fenômenos com impactos às atividades intelectuais nacionais. Analisa conceitos que fundamentam e validam a Bibliografia como área do conhecimento. Atualiza e dá continuidade historiográfica da Bibliografia ao acrescentar reflexões sobre as épocas do trabalho bibliográfico na era digital. Os resultados apontam para a evolução da Bibliografia, tanto enquanto produto e serviço, como área científica em constante desenvolvimento.Conclusões: Através da análise historiográfica, os caminhos da Bibliografia indicam que o campo de estudo é inesgotável, porque a pesquisa sobre o passado de bibliografia acumulada incluem aspectos teóricos que permanecem no tempo atual e sua atualidade expõe muitas possibilidades para o seu futuro. As bibliografias em ambiente Web permitem expandir entendimentos referentes ao acesso e ao uso da informação pela possibilidade de se empregar recursos tecnológicos capazes de aperfeiçoar suas funções sociais de acesso ao conhecimento, em distintos escopos.

  20. Assembling of ({beta}LPH) beta-lypothrophine radioimmunoassay. Plasma levels standardization in normal individuals and patients with hypophysis and adrenals diseases; Montagem do radioimunoensaio da beta-lipotrofina ({beta}LPH). Padronizacao dos niveis plasmaticos em individuos normais e em pacientes com doencas hipofisarias e adrenais

    Energy Technology Data Exchange (ETDEWEB)

    Castro, Margaret de

    1988-12-31

    The present study investigates the extraction and radioimmunoassay (RIA) conditions of plasma {beta}LPH. It was extracted by the activated silicic acid method, with a mean extraction efficiency of 31.6% and a mean intra-extraction variation coefficient of 8.1%. Radioiodination was performed by the chloramine-T method and {beta}LPH {sup 125} I was purified by gel chromatography on Sephadex G100. Estimated specific activity ranged from 100 to 192.8 {mu}Ci/{mu}g, with a mean incorporation percentage of 66.6%. The titer of the first antibody was 1:50.000/100 {mu}l. The assay was performed under non-equilibrium conditions, with a pre-incubation period of 24 hours and incubation of 4 hours. Mean immunoreactivity (Bo/Total) was 21.1%, with a mean Blank/Total ratio of 2.3%. Sensitivity, expressed as the mean minimum detectable dose, was 40 pg/tube, equivalent to 56 pg/ml plasma. Intra-assay variation coefficients were 6.5%, 3.8% and 6.8%, respectively, at B/Bo levels of 0.8, 0.6 and 0.4 of the standard curve. At B/Bo equal to 0.5, the intra-assay variation coefficient was 20.9%. Replicates of 14 plasma samples showed a correlation coefficient of r 0.99, (p< 0.05). Parallelism between the curve obtained with different volumes of an extract with a high {beta}LPH value and the standard curve was found. The method was controlled biologically by the presence of correlation between the plasma {beta}LPH levels and determined pathological states and with clinical functional studies. Twenty seven normal individuals, 10 patients with Cushing`s disease to a tumor of the hypophysis, 4 patients with Cushing syndrome due to an adrenal tumor, 10 patients Addison disease, and 8 patients with hypopituitarism were studied. (author). 119 refs., 28 figs., 2 tabs.

  1. A reforma sanitária brasileira: a vitória sobre o modelo neoliberal

    Directory of Open Access Journals (Sweden)

    Amélia Cohn

    2008-07-01

    Full Text Available O artigo traça um panorama da experiência da reforma do sistema de saúde do Brasil, a partir de meados dos anos 70, apontando seus constrangimentos de natureza estrutural e política, as estratégias políticas e sociais utilizadas, e as propostas da reorganização do sistema de saúde que hoje se concretizam no SUS – Sistema Único de Saúde, um dos maiores sistemas públicos de saúde de mundo. O eixo da exposição e da análise é dado pela constatação de que a Reforma Sanitária brasileira é um exemplo, na América Latina, de que um projeto alternativo para a saúde, democrático, universal, baseado nos preceitos da seguridade social, é viável, e uma vez deflagrado, difícil de ser obstruído pelas forças conservadoras de corte neoliberal.

  2. Artéria coronária direita anômala com origem na artéria pulmonar e pericardite constritiva: uma associação inusitada

    OpenAIRE

    2013-01-01

    A associação da artéria coronária direita anômala com origem na artéria pulmonar e pericardite constritiva ainda não foi descrita na literatura. Apresentamos aqui o primeiro caso dessa associação inusitada em um paciente com quadro de insuficiência cardíaca direita. Após o diagnóstico, o paciente foi encaminhado para tratamento cirúrgico, sendo submetido a pericardiectomia frênico a frênico, implante de enxerto da artéria mamária interna direita para a coronária direita e ligadura da origem a...

  3. Ator de sua trajetória profissional?

    Directory of Open Access Journals (Sweden)

    Geneviève Dahan-Seltzer

    2008-11-01

    Full Text Available Este artigo consiste numa síntese de nossos cursos oferecidos nos cursos de formação continuada do Instituto de Estudos Políticos e da Universidade de Paris, Dauphine, nos quais mobilizamos um conhecimento sociológico que nos permite munir os próprios atores analisados - participantes da pesquisa - com as ferramentas analíticas necessárias para encontrar respostas aos problemas de sua trajetória profissional. Primeiramente, apresentamos a constatação de fatos que nos conduzem àquele método : buscamos apresentar o contexto de crise (dos modelos de identificação, dos vínculos sociais e das instituições e da socialização no qual se inserem atores e empresas. A partir dessa constatação, mobilizamos um conhecimento sociológico, baseado na teoria do ator e da reflexividade, para propor um projeto de pesquisa com fins práticos, de modo a fornecer aos atores em crise as ferramentas necessárias para sua superação, por meio de uma auto-análise, especialmente de sua trajetória e do contexto profissionais no qual evolui. Finalizamos o artigo discutindo esse aspecto aplicado, defendendo a necessidade de uma postura sociológica ao mesmo tempo clínica e crítica.

  4. Campanya solidària Recicla Cultura (2015)

    OpenAIRE

    Universitat de Barcelona. CRAI

    2015-01-01

    Objectiu de l'actuació: Participar activament en la campanya solidària El teu llibre pot canviar vides difonent l’activitat i oferint diferents biblioteques del CRAI com a llocs de recollida de llibres. Descripció del producte: Cartell

  5. Campanya solidària Recicla Cultura 2016

    OpenAIRE

    Universitat de Barcelona. CRAI

    2016-01-01

    Objectius: Participar activament en la campanya solidària El teu llibre pot ser part d’una historia difonent l’activitat i oferint diferents biblioteques del CRAI com a llocs de recollida de llibres. Descripció: Cartell i Infografía.

  6. Memórias perdidas, identidades sem cidadania

    Directory of Open Access Journals (Sweden)

    Ungulani Ba Ka Khosa

    2015-04-01

    Full Text Available Este texto procura contribuir para o debate sobre as políticas identitárias no Moçambique contemporâneo. Contrapondo, através de vários exemplos, a diversidade identitária do tecido social do país à altivez ignorante das estruturas de poder nele presentes, o texto procura problematizar, em vários momentos históricos, a fratura cognitiva entre o projeto político nacional, conceptualizado a partir das elites que controlam o Estado, e o plurilinguismo e a polifonia que constroem o tecido social real. A partir desta dicotomia o texto explora o discurso sobre a nação a várias vozes, problematizando os sentidos das lições da história de Moçambique e os múltiplos silenciamentos que a narrativa histórica dominante vem realizando.

  7. Cultura, História, valores patrimoniais e museus

    Directory of Open Access Journals (Sweden)

    Dominique Poulot

    2011-12-01

    Full Text Available A partir do final do século XVIII até o início da era das nacionalidades, a história dos museus está intimamente ligada à história dos edifícios patrimoniais. O apelo ao nacionalismo popular deu ênfase à formação de coleções capazes de retornar às origens coletivas da nova comunidade imaginária. O museu de história pretende, então, servir de oficina aos historiadores e de espelho de um mundo desaparecido que é necessário reivindicar e também encarnar de maneira convincente. As contradições desses objetivos e dessas abordagens tornaram-se evidentes durante o século XX, alimentando-se das interpretações opostas sobre o papel do museu como lugar de manipulação de patrimônios.

  8. Campanya solidària Recicla Cultura (2015)

    OpenAIRE

    Universitat de Barcelona. CRAI

    2015-01-01

    Objectiu de l'actuació: Participar activament en la campanya solidària El teu llibre pot canviar vides difonent l’activitat i oferint diferents biblioteques del CRAI com a llocs de recollida de llibres. Descripció del producte: Cartell

  9. Campanya solidària Recicla Cultura 2016

    OpenAIRE

    Universitat de Barcelona. CRAI

    2016-01-01

    Objectius: Participar activament en la campanya solidària El teu llibre pot ser part d’una historia difonent l’activitat i oferint diferents biblioteques del CRAI com a llocs de recollida de llibres. Descripció: Cartell i Infografía.

  10. História da vida privada no Brasil

    Directory of Open Access Journals (Sweden)

    Boris Fausto

    2010-07-01

    Full Text Available História da vida privada no Brasil. Vol. 1 de 3. Dirección de Fernando Novais, organización de este volumen: Laura de Mello e Souza. Sao Paulo: Companhia das Letras, 1997. 560 páginas.

  11. Cutis verticis gyrata primária essencial

    Directory of Open Access Journals (Sweden)

    Schenato Letícia K.

    2002-01-01

    Full Text Available Objetivo: relatar um caso raro de uma criança portadora da forma primária essencial de Cutis verticis gyrata.Descrição: menino de 9 anos, apresenta importante hipertrofia da pele do couro cabeludo, com dobras que se assemelham aos giros cerebrais. Não apresenta retardo mental, nem alterações oftalmológicas e não há relatos semelhantes na família.Comentários: o diagnóstico de Cutis verticis gyrata primária essencial foi estabelecido pela presença de redundância da pele do couro cabeludo e ausência de alterações neurológicas e oftalmológicas, tendo-se feito o diagnóstico diferencial com as formas secundárias que incluem: nevo intradérmico cerebriforme, paquidermoperiostose, acromegalia e doenças inflamatórias do couro cabeludo. Trata-se do único relato na literatura de uma criança com esta forma de Cutis verticis gyrata.

  12. Memória e utopia na cena teatral

    Directory of Open Access Journals (Sweden)

    Sergio Miceli

    2012-11-01

    Full Text Available Memória e utopia, transmutadas em linguagem teatral, renovam a apreensão da relação entre dramaturgia e experiência social. Em A moratória (1955 e Rastro atrás (1966, a lembrança do descenso da família de Jorge Andrade impregna a fala dos personagens e dos objetos que os cercam. Neles se inscreve a história da família e da oligarquia agrária a que pertenceu o dramaturgo. A peça de Gianfrancesco Guarnieri, Eles não usam black-tie (1958, ativou os sonhos de uma geração sobre o potencial da cultura na reordenação da sociedade. O operariado estreou nos palcos da metrópole pelo drama de uma família tensionada pela greve, pelo conflito geracional e pela luta de classes. Eis a prova do relevo da cena teatral numa conjuntura excepcional de transformação da cultura brasileira, sinalizada pelo cinema novo e pelo intrincado entrelaçamento do teatro, com o rádio e com o início da televisão no país.

  13. Studies on the mode of action of calciferol. XIII. Development of a radioimmunoassay for vitamin D-dependent chick intestinal calcium-binding protein and tissue distribution

    Energy Technology Data Exchange (ETDEWEB)

    Christakos, S.; Friedlander, E.J.; Frandsen, B.R.; Norman, A.W.

    1979-05-01

    A RIA for chick intestinal calcium-binding protein (CaBP) has been developed with a sensitivity of 1 ng. The antiserum was generated in rabbits injected with highly purified vitamin D-dependent chick intestinal CaBP. The assay employs the double antibody technique, and /sup 125/I-labeled CaBP was prepared using chloramine T. Low molecular weight peptide hormones and normal rabbit, rat, and human serum proteins show no cross-reactivity in the assay. Measurements of chick intestinal and kidney CaBP by RIA showed a good correlation with measurements of CaBP by the radial immunodiffusion method. The assay is reproducible (interassay variability, 16.3%) and precise (intraassay variability, 4.0%). The concentration of immunoreactive CaBP (iCaBP) in chick serum (2.7 ng/ml serum) can now be measured as early as 8 h after the administration of 6.5 nmol 1,25-dihydroxyvitamin D/sub 3/; a maximum of 11 ng/ml is reached at 20 h. The level of CaBP in chick serum was found to be dependent on the dose of vitamin D/sub 3/ or 1,25-dihydroxyvitamin D/sub 3/ administered to the animal.

  14. Radioimmunoassays of hidden viral antigens

    Energy Technology Data Exchange (ETDEWEB)

    Neurath, A.R. (Lindsley F. Kimbell Research Inst., New York, NY); Strick, N.; Baker, L.; Krugman, S.

    1982-07-01

    Antigens corresponding to infectious agents may be present in biological specimens only in a cryptic form bound to antibodies and, thus, may elude detection. We describe a solid-phase technique for separation of antigens from antibodies. Immune complexes are precipitated from serum by polyethylene glycol, dissociated with NaSCN, and adsorbed onto nitrocellulose or polystyrene supports. Antigens remain topographically separated from antibodies after removal of NaSCN and can be detected with radiolabeled antibodies. Genomes from viruses immobilized on nitrocellulose can be identified by nucleic acid hybridization. Nanogram quantities of sequestered hepatitis B surface and core antigens and picogram amounts of hepatitis B virus DNA were detected. Antibody-bound adenovirus, herpesvirus, and measles virus antigens were discerned by the procedure.

  15. Radioimmunoassays of hidden viral antigens.

    Science.gov (United States)

    Neurath, A R; Strick, N; Baker, L; Krugman, S

    1982-01-01

    Antigens corresponding to infectious agents may be present in biological specimens only in a cryptic form bound to antibodies and, thus, may elude detection. We describe a solid phase technique for separation of antigens from antibodies. Immune complexes are precipitated from serum by polyethylene glycol, dissociated with NaSCN, and adsorbed onto nitrocellulose or polystyrene supports. Antigens remain topographically separated from antibodies after removal of NaSCN and can be detected with radiolabeled antibodies. Genomes from viruses immobilized on nitrocellulose can be identified by nucleic acid hybridization. Nanogram quantities of sequestered hepatitis B surface and core antigens and picogram amounts of hepatitis B virus DNA were detected. Antibody-bond adenovirus, herpesvirus, and measles virus antigens were discerned by the procedure. Images PMID:6956871

  16. Radioimmunoassay method for measurement of plasma androstenedione. Its validation in ovulatory women and in patients with polycystic ovarian syndrome; Metodo de radioimunoensaio para medida da androstenediona plasmatica. Validacao em mulheres ovulatorias e com sindrome dos ovarios policisticos

    Energy Technology Data Exchange (ETDEWEB)

    Vilanova Socorro Veras, Maria do; Silva e Rosa, Alzira Amelia; Moura, Marcos Dias de; Ferriano, Rui Alberto; Sa, Marcos Felipe Silva de [Sao Paulo Univ., Ribeirao Preto, SP (Brazil). Faculdade de Medicina

    1995-01-01

    The present paper has as objective the standardization of a radioimmunoassay method for measurement of androstenedione. Ethyl ether was used for plasma extraction. The sensitivity of the method was 6,8 pg/tube; the reproducibility (inter assay error) was 15,6%; the precision (intrassay error) was 5,2%. As biological control, 20 ovulatory women showed median plasma values of 1250 pg/ml and 24 women with polycystic ovary syndrome presented median plasma values of 2.037 pg/ml. (author). 6 refs., 2 figs., 1 tab.

  17. A antessala de Siegfried Kracauer e Cees Nooteboom: Memória, história e imagem

    Directory of Open Access Journals (Sweden)

    Samanta Lopes Bergé

    2014-06-01

    Full Text Available http://dx.doi.org/10.5007/2175-7917.2014v19n1p134 Tendo como ponto de partida o romance Dia de Finados do escritor neerlandês Cees Nooteboom e History – the last things before the last, de Siegfried Kracauer, o presente ensaio tem como objetivo pensar as relações entre memória, história, imagem e o impacto das novas tecnologias nos modos de ver e ler. A memória e os subsequentes paralelos traçados por Kracauer entre filosofia e história, arte e fotografia/cinema serão abordados levando em consideração o momento atual da era digital e da mobilidade onde a televisão passa a ser vista, pouco a pouco, apenas como mero suporte. Da explosão do seriado americano True Detective ao inglês Black Mirror, o artigo busca fazer uma releitura do Culto da distração, publicado no jornal Frankfurter Zeitung em meados da década de 20 do século passado. As relações entre o historiador de Kracauer e o personagem principal do livro de Nooteboom também vêm à tona nos anjos de Wim Wenders, homens com asas, monstros histórico-literários.

  18. Manifestações respiratórias na malária por Plasmodium falciparum e Vivax: vivax

    Directory of Open Access Journals (Sweden)

    C. Botelho

    1987-12-01

    Full Text Available Foram selecionados 110 pacientes que procuraram a SUCAM/CUIABÁ para diagnóstico e tratamento de malária, com queixa de febre e procedente de área malarígena. Nestes pacientes foram realizados: gota espessa para a pesquisa de Plasmodium; aplicação de questionário padronizado para detectar sintomas respiratórios presentes no momento da entrevista; gasometria arterial e espirometria para avaliar a função pulmonar. Desta amostra, 62 pacientes foram positivos para malária sendo 41 P. vivax, 20 P. falciparum e 01 forma mista (grupo Malárico; 48 mostraram-se negativos à pesquisa de hematozoários através da gota espessa (grupo Não Malárico. As manifestações respiratórias encontradas nesta população quando comparadas os grupos Malárico e Não Malárico, foram respectivamente: 1º Sintomáticos Respiratórios de 46,8% para 22 9% (p < 0,05; 2° Alterações Espirométricas de 53,2% para 20,8% (p < 0,05; 3° Alterações Gasométricas de 62,9% para 25,0% (p < 0,05. Com estes resultados concluímos que há nítida associação entre a Malária produzida pelo P. vivax e P. falciparum e as manifestações respiratórias apresentadas, nesta população de estudo.

  19. Aprendizado e memória Learning and memory

    Directory of Open Access Journals (Sweden)

    Paul Lombroso

    2004-09-01

    Full Text Available A memória é dividida de duas grandes formas: explícita e implícita. O hipocampo é necessário para a formação das memórias explícitas, ao passo que várias outras regiões do cérebro, incluindo o estriado, a amígdala e o nucleus accumbens, estão envolvidos na formação das memórias implícitas. A formação de todas as memórias requer alterações morfológicas nas sinapses: novas sinapses devem ser formadas ou antigas precisam ser fortalecidas. Considera-se que essas alterações reflitam a base celular subjacente das memórias persistentes. Consideráveis avanços têm ocorrido na última década em relação a nossa compreensão sobre as bases moleculares da formação dessas memórias. Um regulador-chave da plasticidade sináptica é uma via de sinalização que inclui a proteína-quinase ativada por mitógenos (MAP. Como essa via é necessária para a memória e o aprendizado normais, não é surpreendente que as mutações nos membros dessa via levem a prejuízos no aprendizado. A neurofibromatose, a síndrome de Coffin-Lowry e a de Rubinstein-Taybi são três exemplos de transtornos de desenvolvimento que apresentam mutações em componentes-chave na via de sinalização da proteína-quinase MAP.Memory is broadly divided into declarative and nondeclarative forms of memory. The hippocampus is required for the formation of declarative memories, while a number of other brain regions including the striatum, amygdala and nucleus accumbens are involved in the formation of nondeclarative memories. The formation of all memories require morphological changes of synapses: new ones must be formed or old ones strengthened. These changes are thought to reflect the underlying cellular basis for persistent memories. Considerable advances have occurred over the last decade in our understanding of the molecular bases of how these memories are formed. A key regulator of synaptic plasticity is a signaling pathway that includes the mitogen

  20. Produção territorial das reformas agrárias no Brasil

    OpenAIRE

    Rocha, Herivelto Fernandes [UNESP

    2013-01-01

    A Reforma Agrária no Brasil é um debate atual que necessita ser realizado. A diversidade dos territórios produzidos por esta política pública a partir das várias políticas de obtenção de terras, nos motivou a realizar este estudo. Nele vimos que a Reforma Agrária brasileira perpassa por ao menos três possibilidades, a saber: a Reforma Agrária Estrutural, que faz parte das reinvindicações dos movimentos socioterritoriais; a Reforma Agrária Marginal, implementada principalmente pelos governos m...

  1. Reactivity Insertion Accident (RIA) Capability Status in the BISON Fuel Performance Code

    Energy Technology Data Exchange (ETDEWEB)

    Williamson, Richard L. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Folsom, Charles Pearson [Idaho National Lab. (INL), Idaho Falls, ID (United States); Pastore, Giovanni [Idaho National Lab. (INL), Idaho Falls, ID (United States); Veeraraghavan, Swetha [Idaho National Lab. (INL), Idaho Falls, ID (United States)

    2016-05-01

    One of the Challenge Problems being considered within CASL relates to modelling and simulation of Light Water Reactor LWR) fuel under Reactivity Insertion Accident (RIA) conditions. BISON is the fuel performance code used within CASL for LWR fuel under both normal operating and accident conditions, and thus must be capable of addressing the RIA challenge problem. This report outlines required BISON capabilities for RIAs and describes the current status of the code. Information on recent accident capability enhancements, application of BISON to a RIA benchmark exercise, and plans for validation to RIA behavior are included.

  2. Marx e a História em Ruy Fausto

    Directory of Open Access Journals (Sweden)

    Carlos Prado

    2011-01-01

    Full Text Available

    Resumo: O objetivo desse artigo é expor as considerações de Fausto sobre a problemática da história em Marx. Fausto escreve em sua Lógica e política importantes considerações sobre o tema, afirmando que Marx não deixou uma filosofia da história, tampouco uma teoria da história, mas apenas uma apresentação da história. Na qual Fausto identifica três modelos: História da liberdade; História da riqueza e História da satisfação.

    Palavras-chave: História; Dialética, modelos.

     

    Abstract: The objective of this article is to expose Fausto's considerations around the questions in Marx' history. Fausto writes his Logic and Politic in important considerations around the issue, affirming that Marx hasn't left a philosophy of the history, neither a theory of the history, but simply a presentation of the history. In witch Fausto identify three patterns: History of the freedom; History of the wealth and History of the satisfaction.

    tortura carcerária : sobrepena do sistema penal brasileiro

    OpenAIRE

    2013-01-01

    Essa dissertação é um estudo multidisciplinar que consiste na revisão de literatura-bibliográfica acerca do objeto: tortura carcerária e a analisa como sobrepena privativa de liberdade do subterrâneo sistema penal brasileiro. Donde mantém relações particulares, próprias e específicas com as agências que fazem e movem de modo desarmônico tal sistema: as agências políticas promovem a programação constitucional e legal que definem o contorno do crime de tortura, as judiciárias enclausuradas e...

  3. Cross-Section Measurements with the Radioactive Isotope Accelerator (RIA)

    Energy Technology Data Exchange (ETDEWEB)

    Stoyer, M A; Moody, K J; Wild, J F; Patin, J B; Shaughnessy, D A; Stoyer, N J; Harris, L J

    2002-11-19

    RIA will produce beams of exotic nuclei of unprecedented luminosity. Preliminary studies of the feasibility of measuring cross-sections of interest to the science based stockpile stewardship (SBSS) program will be presented, and several experimental techniques will be discussed. Cross-section modeling attempts for the A = 95 mass region will be shown. In addition, several radioactive isotopes could be collected for target production or medical isotope purposes while the main in-beam experiments are running. The inclusion of a broad range mass analyzer (BRAMA) capability at RIA will enable more effective utilization of the facility, enabling the performance of multiple experiments at the same time. This option will be briefly discussed.

  4. Reliability and Availability Studies in the RIA Linac Driver

    CERN Document Server

    Schnirman-Lessner, Eliane

    2005-01-01

    The RIA facility will include various complex systems and must provide radioactive beams to many users simultaneously. The availability of radioactive beams for most experiments at the fully-commissioned facility should be as high as possible within design cost limitations. To make a realistic estimate of the achievable reliability a detailed analysis is required. The RIA driver linac is a complex machine containing a large number of SC resonators and capable of accelerating multiple-charge-state beams. At the pre-CDR stage of the design it is essential to identify critical facility subsystem failures that can prevent the driver linac from operating. The reliability and availability of the driver linac are studied using expert information and data from operating machines such as ATLAS, APS, JLab, and LANL. Availability studies are performed with a Monte-Carlo simulation code previously applied to availability assessments of the NLC facility [http://www.slac.stanford.edu/xorg/accelops/Full/LCoptsfull] and the ...

  5. Arte da memória e arquitetura

    OpenAIRE

    Renata La Rocca

    2007-01-01

    Partindo da hipótese de que o uso da arte da memória pode ser compreendido como um sistema-processo de design para a concepção de espaços no contexto da cultura digital, o objetivo do presente trabalho é construir uma compreensão ampliada do design de espaços que dão suporte ao armazenamento estruturado de informações, relacionando imagens e lugares. A investigação contempla o uso da arte da memória na concepção de espaços e seus princípios fundamentais desde a antiguidade clássica até os dia...

  6. Hiperplasia fibroepitelial mamária em felinos: cinco casos

    Directory of Open Access Journals (Sweden)

    Souza Tatiana Mello de

    2002-01-01

    Full Text Available São descritos cinco casos de hiperplasia fibroepitelial mamária em gatas com idades variando entre 10 meses e 11 anos. A principal queixa por ocasião do atendimento era relacionada à tumefação das mamas com evolução clínica entre 3 e 24 semanas. Em duas das gatas, a condição desenvolveu-se após o tratamento com contraceptivos. Macroscopicamente, as tumorações na glândula mamária eram cobertas por pele, ocasionalmente ulceradas, e tinham superfície de corte brancacenta com nódulos multifocais mais pálidos de 2mm de diâmetro. Histologicamente, esses nódulos multifocais consistiam de proliferação ductal cercadas por crescimento abundante de tecido conjuntivo fibroso.

  7. Sobre o editor: notas para sua história

    Directory of Open Access Journals (Sweden)

    Anibal Bragança

    2005-12-01

    Full Text Available O artigo procura contribuir para a definição da função editor de livros impressos e oferece indicações básicas de sua história no Ocidente. Etimologia e conceitos.Tipologia histórica. Formação, desenvolvimento e crise da cultura impressa. Mudanças e novas oportunidades para a função editor na contemporaneidade.

  8. O percurso de uma pesquisa em História

    Directory of Open Access Journals (Sweden)

    Maria Siqueira Santos

    2012-03-01

    Full Text Available Esse texto é o relato da pesquisa que empreendi quando realizava o curso de Mestrado em História Social na Universidade Estadual de Londrina. Iniciei a pesquisa estudando o romance Nove noites de Bernardo Carvalho, embrenhei-me pelo labirinto de suas vozes narrativas e envolvi-me na discussão a respeito de sua metaficcionalidade. Em seguida, analisei teses acadêmicas que focalizavam tais metaficções. Queria visualizar o discurso acadêmico acerca da literatura contemporânea, perceber se tais trabalhos poderiam ser classificados como metaficcionais. Ao ler as teses, porém, descobri António Lobo Antunes. Autor de vários romances, esse português começou sua produção literária com Memória de elefante, cuja trama se desenrola ao longo do dia de um psiquiatra que encontra na linguagem a possibilidade de transformar o mundo. Quando pensava finalmente ter encontrado minha fonte histórica, um episódio casual, o encontro com um livro, mudou a direção de minha pesquisa. Escrito pelo filósofo Peter Sloterdijk, A árvore mágica conta a história do magnetismo animal; é “uma tentativa épica com relação à filosofia da psicologia.” O presente texto, por ser um relato do percurso da pesquisa de fontes, termina no momento em que descobri esse romance e pude delimitar o assunto a ser tratado em meu texto dissertativo.

  9. Cascas sobre o papel: memória do dilaceramento

    Directory of Open Access Journals (Sweden)

    Vera Casa Nova

    2014-08-01

    Full Text Available O ensaio tenta mostrar como o autor Georges Didi-Huberman, na obra Écorces (Cascas, nos apresenta sua experiência de rastros (W. Benjamin e sua interpretação do dilaceramento nos campos de Auschwitz Birkenau.Trata-se de uma narrativa de ruínas pela palavra e pela imagem (fotografia. Escritura que se encontra entre a pesquisa histórica e a empresa literária autobiográfica.

  10. História, memória e narrativa: a revelação do "quem" nas histórias orais dos habitantes do Córrego dos Januários

    OpenAIRE

    Gusmão,Denise Sampaio; Jobim e Souza,Solange

    2010-01-01

    O objetivo deste artigo é relatar uma pesquisa sobre as histórias de vida e as memórias dos habitantes de um pequeno povoado, situado na região leste de Minas Gerais, Brasil. Utilizando a "história oral" como estratégia metodológica e tendo como referência teórica o pensamento de Hannah Arendt, Walter Benjamin e Mikhail Bakhtin, os conceitos história, memória e narrativa são aqui analisados a partir das práticas discursivas entre a pesquisadora e os habitantes do Córrego dos Januários. Os res...

  11. Heavy-ion LINAC development for the US RIA project

    Indian Academy of Sciences (India)

    P N Ostroumov

    2002-12-01

    The Nuclear Science Community in the Unites States has unanimously concluded that developments in both nuclear science and its supporting technologies make building a world-leading Rare-Isotope Accelerator (RIA) facility for production of radioactive beams the top priority. The RIA development effort involves several US Laboratories (ANL, JLAB, LBNL, MSU, ORNL). The RIA facility includes a CW 1.4 GeV driver LINAC and a 100 MV post-accelerator both based on superconducting (SC) cavities operating at frequencies from 48 MHz to 805 MHz. An initial acceleration in both LINACs is provided by room temperature RFQs. The driver LINAC is designed for acceleration of any ion species; from protons up to 900 MeV to uranium up to 400 MeV/u. The novel feature of the driver LINAC is an acceleration of multiple charge-state heavy-ion beams in order to achieve 400 kW beam power. Basic design concepts of the driver LINAC are given. Several new conceptual solutions in beam dynamics, room temperature and SC accelerating structures for heavy ion accelerator applications are discussed.

  12. Quando a História vira entretenimento

    Directory of Open Access Journals (Sweden)

    Michelli Machado

    2010-10-01

    Full Text Available O texto busca fazer algumas considerações sobre as releituras midiáticas da história, propostas por minisséries de época apresentadas pela Rede Globo. A partir de uma observação desses fenômenos midiáticos, buscaremos refletir sobre a relação entre mídia, entretenimento e midiatização da narrativa histórica nas minisséries. Autores como Lopes serão essenciais para entendermos os mecanismos das séries de ficção, enquanto Martín-Barbero nos ajudará a pensar as amarras identitárias que existem entre esses programas de entretenimento e a história do país, trazidas à tona para discussão na sociedade contemporânea por meio das minisséries.

  13. Histórias em quadrinhos: formando leitores

    Directory of Open Access Journals (Sweden)

    Mariana Oliveira dos Santos

    Full Text Available O foco do presente estudo são as histórias em quadrinhos e as possibilidades de incentivo de sua leitura na escola e na biblioteca. A pesquisa tem caráter bibliográfico, qualitativo e exploratório, resultante de um levantamento de projetos e trabalhos que utilizaram os quadrinhos na formação de leitores. Além da trajetória histórica dos quadrinhos no Brasil e de suas características de linguagem, buscou-se identificar, nos projetos e trabalhos selecionados, a participação do bibliotecário e do professor como mediadores entre os quadrinhos e as crianças. A pesquisa confirma a hipótese de que a história em quadrinhoo é um recurso muito eficiente como incentivo à leitura, além de um importante auxiliar no ensino, contribuindo para a formação de leitores mais competentes.

  14. A memória dos idosos em tarefas complexas

    Directory of Open Access Journals (Sweden)

    Terezinha de Jesus C. G. van Erven

    Full Text Available Dois experimentos compararam o desempenho de jovens e idosos nos testes de recuperação livre, recuperação com pista extra-lista e recuperação com pista intra-lista. No primeiro experimento, foi avaliada a influência da complexidade da tarefa, do tempo de apresentação dos estímulos e da faixa etária sobre a recuperação livre de palavras apresentadas no contexto de sentenças. No segundo, jovens e idosos foram comparados para verificar os efeitos da complexidade da tarefa, do tipo de teste, do tamanho do conjunto do alvo e da força associativa da pista sobre a memória de palavras no contexto de sentenças do teste de recuperação com pista extra-lista e intra-lista. Os resultados mostraram uma redução na evocação em função do aumento da idade dos participantes. Também indicaram que a magnitude da diferença variou, dependendo do tipo de teste de memória aplicado e da presença de contexto relacionado ao alvo durante a codificação.

  15. O livro nas memórias de leitura

    Directory of Open Access Journals (Sweden)

    Ilsa do Carmo Vieira Goulart

    2011-06-01

    Full Text Available Com o objetivo de averiguar quais sentidos e valores poderiam estar agregados a um objeto-livro no decorrer do tempo, este trabalho propõese a analisar o vínculo que se estabelece na relação entre o leitor e a leitura por intermédio do livro. A pesquisa utiliza-se das memórias de experiências de leitura reconstituídas de um tempo da vida em que o livro esteve presente, a partir de relados de pessoas acima de 50 anos que guardaram seu material de leitura durante décadas, ou mesmo daquelas que se sentiram movidas a reencontrar este material, ou ainda de quem guarda o livro de outrem. O trabalho subsidia-se nos estudos de Certeau (1994 e de Bosi (1994 sobre memória e nos estudos de Chartier (1994, 1996, 1999, referentes à história do livro.

  16. Situações postas à história

    Directory of Open Access Journals (Sweden)

    François Hartog

    2012-06-01

    Full Text Available O objetivo principal do artigo é refletir sobre a história como conceito e como prática, além de interrogá-la como crença socialmente compartilhada. Nessa longa história de uma crença, com seus tempos de certeza e outros de dúvida, dois momentos serão privilegiados neste artigo. Em primeiro lugar, os meados do século XX, quando, logo após o fim da Segunda Guerra Mundial, a evidência da história será rapidamente reafirmada. Em segundo lugar, o momento atual, encarado como aquele em que a evidência se esfumaça e em que a crença se fissura. Tratar-se-á, portanto, de refletir sobre a resistência de um dos conceitos centrais do mundo moderno e de algumas de suas transformações.

  17. Desconstruir para problematizar matrizes identitárias

    Directory of Open Access Journals (Sweden)

    Mériti de Souza

    Full Text Available A rede social contemporânea encontra-se marcada pela construção e pela disseminação de matrizes identitárias configuradas por binarismos, hierarquias e atribuição de específicos sentidos às configurações subjetivas. Com este estudo, objetiva-se problematizar as possibilidades de desconstrução dessas matrizes concomitantemente ao deslocamento de sentidos a elas agregados. Os aportes teóricos de Butler e Derrida possibilitam compreender que as referências identitárias fundamentadas na tradição filosófica ocidental da metafísica da presença engendram o enunciado da subjetividade e os sentidos agregados ao masculino e ao feminino. A construção do enunciado do gênero como identidade possibilita aos sujeitos se reconhecerem em referência às suas práticas de sexualidade e à heteronormatividade, que estabelece tanto as oposições masculino/feminino, heterossexual/homossexual quanto a hierarquia entre esses pares. Entende-se que o deslocamento dos sentidos atribuídos aos pares binários pode ocorrer a partir da desconstrução dos pressupostos que organizam as matrizes identitárias dominantes na rede social.

  18. Impossibilidade de alteração de lei complementar por lei ordinária em matéria tributária

    Directory of Open Access Journals (Sweden)

    Marcelo de Lima Castro Diniz

    2000-03-01

    Full Text Available Usually the legislator controls a specific subject through a complementary law, although it can be done through a ordinary law, which is different concerning the legislative process, mainly on the quorum matter. A law with these characteristics must be considered complementary law, so that its modification is only possible by a norm on the same level. According to the principles of “more value legitimatory” and liberty of conformation of the legislator in his legislative activity.É comum que o legislador regule um determinado assunto por meio de lei complementar, embora pudesse fazê-lo através de lei ordinária, cujo processo legislativo é diferente, sobretudo pela questão do quorum. Uma lei com essas características deve ser considerada lei complementar, decorrendo daí que sua modificação apenas é possível por norma do mesmo escalão, em face dos princípios da mais valia legitimatória e da liberdade de conformação do legislador na atividade legislativa.

  19. História e filosofia da história na obra do jovem Friedrich Schlegel

    Directory of Open Access Journals (Sweden)

    Izabela Maria Furtado Kestler

    2008-01-01

    Full Text Available Este artigo apresenta e comenta dois textos do jovem Friedrich Schlegel, Vom Wert des Studiums der Griechen und Römer (Sobre o valor do estudo dos gregos e romanos, de 1795/1796, e Über das Studium der griechischen Poesie (Sobre o estudo da poesia grega, de 1795, escritos antes de sua ida para Jena, em 1796, nos quais o autor elabora uma teoria original sobre a história e a filosofia da história.This article introduces and comments two texts written by Friedrich Schlegel in his youth, before he moved to Jena in 1796: Vom Wert des Studiums der Griechen und Römer (On the value of the study of Greeks and Romans of 1795/1796 and Über das Studium der griechischen Poesie (On the study of Greek poetry of 1795. In these texts the author develops an original theory concerning history and the philosophy of history.

  1. A Andragogía Universitária na docência Universitária

    OpenAIRE

    Guillén Contreras, Bernal

    2014-01-01

    O artigo trata da Andragogia Profissional Universitária, propõe-se e se projeta em função do estímulo para a gestão das aprendizagens significativas. À luz dos diversos fatos, requerem–se formas heurísticas para a abordagem da mediação e facilitação das aprendizagens nas salas de aula universitárias. Portanto, os docentes universitários, têm a obrigação ético–profissional de regenerar–se, oxigenar–se, redesenhar os processos da aprendizagem, para ser competitivos perante as gerações da atuali...

  2. Alguns apontamentos sobre a trajetória da Psicologia social comunitária no Brasil

    Directory of Open Access Journals (Sweden)

    Mariana Alves Gonçalves

    Full Text Available Este artigo aborda a trajetória da Psicologia social comunitária no Brasil tomando como material privilegiado de análise os anais dos encontros nacionais da Associação Brasileira de Psicologia Social (ABRAPSO. O objetivo foi o de historiar os argumentos produzidos por autores da PSC em busca de uma identidade para a área. A análise buscou evidenciar a especificidade da PSC, os objetivos de suas intervenções e a noção de comunidade. Tomamos os anais dos encontros nacionais da ABRAPSO como fonte principal pela importância da associação na produção de dispositivos conceituais e de novas formas de atuação profissional dos psicólogos no Brasil. Concluímos o trabalho indicando a ênfase na diferença e na oposicão entre uma psicologia latino e norte-americana como recurso estratégico de afirmação identitária da área. Apontamos a importância do trabalho em comunidade para a renovação das práticas do psicólogo no Brasil e para a relativa carência de reflexão conceitual sobre a noção de comunidade.

  3. A memória do acontecido e a memória-acontecimento: um estudo semiótico dos gêneros autobiográficos

    Directory of Open Access Journals (Sweden)

    Mariana Luz Pessoa de BARROS

    Full Text Available RESUMO Com base nas noções de campo de presença e de acontecimento, desenvolvidas pela gramática tensiva, são propostas duas formas de memória como categoria analítica dos discursos autobiográficos: a memória do acontecido e a memória-acontecimento. Essas organizações discursivas da memória determinam modos diferentes de adesão do enunciatário aos discursos, uma vez que a primeira coloca em cena estratégias que privilegiam a legibilidade do texto e a segunda explora sua dimensão sensorial e afetiva. Tendo isso em vista, o objetivo central deste artigo é investigar, no quadro teórico da semiótica discursiva, a interação entre enunciador e enunciatário em diferentes gêneros autobiográficos, como a autobiografia literária em prosa, os poemas de caráter autobiográfico e os memoriais acadêmicos. Em cada gênero, a memória do acontecido e a memória-acontecimento se articulam de forma singular. É justamente a tensão que se estabelece entre essas duas memórias, entre essas duas formas de conhecer e produzir o mundo, que parece ser fundadora dos discursos autobiográficos.

  4. Indicadores anatômicos de dominância entre as artérias coronárias em caprinos

    Directory of Open Access Journals (Sweden)

    João Leão Pinto Neto

    2009-02-01

    Full Text Available O interesse crescente em se utilizar artérias coronárias de caprinos em experiências como uma etapa para sua aplicação em coronárias humanas justifica o conhecimento anatômico mais detalhado dessas artérias principalmente para se avaliar as semelhanças e as diferenças. Em corações humanos utilizando-se a crux cordis e a procedência do ramo interventricular posterior como referências consideram-se três tipos de padrões: dominância direita, balanceada e dominância esquerda. No entanto, não encontramos citações na literatura sobre os indicadores anatômicos de dominância coronária em caprinos. Material e método: Utilizamos 31 corações de caprinos de ambos os sexos, fixados em formalina a 10%. Os ramos das artérias coronárias eram dissecados com especial atenção para os níveis considerados como referências. O ramo presente no interior do sulco interventricular subsinuoso procedia do ramo circunflexo, ramo da artéria coronária esquerda. O ramo interventricular subsinuoso apresentou pelo menos um pequeno ramo que ultrapassava a crux cordis. A região do ápice apresentou predominância dos ramos da artéria coronária esquerda: interventricular paraconal e interventricular subsinuoso. A artéria coronária esquerda, através de seus ramos, também predominou no comprimento total e na emissão de ramos ventriculares inclusive para o ventrículo direito. Utilizando como referências os diversos critérios inclusive, os propostos por esta pesquisa, concluímos que em corações de caprinos o padrão arterial é dominância esquerda. A diversidade em relação ao padrão arterial entre caprinos e humanos requer que nas experiências efetuadas, em coronárias de caprinos, essa importante diferença seja observada.

  5. Limites e contribuições da história oral: a memória e a história nas interseções entre o individual e o coletivo

    Directory of Open Access Journals (Sweden)

    Willian Eduardo Righinni de Souza

    2010-06-01

    Full Text Available O objetivo deste artigo é analisar os limites e contribuições da história oral para uma discussão sobre a fronteira entre o individual e o coletivo na construção mnemônica, permitindo, entre outras coisas, um questionamento sobre a idéia de autonomia e/ ou dominação/ reprodução discursiva nos relatos produzidos. Ao mesmo tempo, pretende-se abordar a possibilidade da narrativa elaborada pela história oral incentivar nos entrevistados uma reflexão crítica sobre a sociedade e suas experiências pessoais, demonstrando suas especificidades na escrita da história. Em relação ao papel da memória, analisamos as diferenças de pensamento entre Maurice Halbwachs, com sua teoria dos quadros sociais de memória, e Walter Benjamin/ Hannah Arendt, que identificam, na modernidade, o declínio da experiência coletiva e tradição. Já sobre a construção de uma narrativa por meio de entrevistas, dialogamos com Michel de Certeau, Paul Veyne e Carlo Ginzburg para uma discussão sobre a validade de alguns tópicos da teoria da história para o contexto de produção da história oral.

  6. Microfilter paper method for 17. cap alpha. -hydroxyprogesterone radioimmunoassay: its application for rapid screening for congenital adrenal hyperplasia. [Tritium tracer techniques

    Energy Technology Data Exchange (ETDEWEB)

    Pang, S.; Hotchkiss, J.; Drash, A.L.; Levine, L.S.; New, M.I.

    1977-11-01

    A new micromethod for measuring a steroid in blood collected on filter paper has been developed. The method is easy and rapid and has the specificity, accuracy and precision of RIA in whole plasma. Less than 20 ..mu..l of blood is required, and, therefore, samples may be obtained with heel prick. This method has been applied to the determination of 17..cap alpha..-hydroxyprogesterone (17..cap alpha..-OH-P) for screening patients with congenital adrenal hyperplasia (CAH) due to 21-hydroxylase deficiency. There was excellent correlation (r = .94) between the values of 17..cap alpha..-OH-P obtained by microfilter paper method and those from plasma samples of cord (40 +- 13 ng/ml) and neonatal blood (<3.6 ng/ml) in normal infants. In six neonates at risk for CAH the diagnosis was made utilizing the microfilter paper method. 17..cap alpha..-OH-P concentrations were highly elevated in both filter paper eluates of whole blood (67 to 360 ng/ml of plasma) and simultaneously obtained plasma concentration (74 to 395 ng/ml) in affected infants. The concentrations of 17..cap alpha..-OH-P remained unchanged in dried filter paper blood when stored at room temperature for up to 21 days. Thus, filter paper with dried blood may be sent for steroid assay by mail. The ease with which samples may be transported and the minute amount of sample necessary make this method a promising screening test for CAH.

  7. Reatividade vascular da artéria mamária interna: estudos farmacológicos comparativos entre artérias caninas direita e esquerda

    Directory of Open Access Journals (Sweden)

    EVORA Paulo Roberto B.

    1999-01-01

    Full Text Available Para estudos comparativos da reatividade vascular entre artéria mamária interna (AMI canina direita e esquerda, realizaram-se experimentos "in vitro" utilizando-se banhos orgânicos ("organ chambers" e ensaios biológicos: 1 os produtos plaquetários ADP e 5-HT induziram, respectivamente, vasodilatação dependente e independente do endotélio; 2 os autacóides, bradicinina e histamina, também induziram vasodilatação, respectivamente, dependente e independente do endotélio; 3 o A23187, vasodilatador independente de receptor, induziu relaxamentos dependentes do endotélio; 4 dopamina, dobutamina, papaverina e a poli-L-arginina induziram vasodilatações independentes do endotélio; 5 a NOR induziu intensa vasoconstrição comparável à causada pelo KCI e pela endotelina; 6 em 83% de 24 ensaios, a liberação basal de NO foi maior na AMI esquerda, em comparação com a AMI direita; 7 ensaios biológicos de AMIs demonstraram a importância da PGI2 nas condições de hipóxia, uma vez que a indometacina aboliu vasodilatação adicional em resposta à hipóxia em condições de vasodilatação induzida pela liberação basal de NO; 8 não ocorreram diferenças significantes de resposta, comparando-se estudos realizados em AMIs direita e esquerda.

  8. A União Bancária resolve?

    Directory of Open Access Journals (Sweden)

    Nuno Cunha Rodrigues

    2016-05-01

    Full Text Available O presente artigo corresponde ao texto da intervenção realizada no dia 26 de janeiro de 2016, na Faculdade de Direito de Lisboa, na conferência organizada pelo IDEFF e pelo Instituto Europeu subordinada ao tema “O Sistema Financeiro Português”. Partindo da análise dos três pilares da união bancária, o artigo reflete sobre a resolução de dois bancos em Portugal – o BES (Novo Banco e o BANIF – bem como sobre o surgimento daquela união no contexto da crise financeira, concluindo que, sem uma verdadeira união económica e monetária, ainda por concretizar, a união bancária não permitirá resolver alguns dos problemas estruturais da União Europeia. | "Does the Banking Union solve?" The article is the text of the speech held on January 26, 2016, at the Law School of Lisbon, at the conference organized by IDEFF and the European Institute entitled "The Portuguese financial system." Based on the analysis of the three pillars of the banking union, the article reflects on the resolution of two banks in Portugal - BES (Novo Banco and BANIF - as well as the emergence of the banking union in the context of the financial crisis, concluding that without true economic and monetary union, yet to be achieved, the banking union will not be able to solve some of the structural problems of the European Union.

  9. Hipertensão refratária e hiperaldosteronismo

    Directory of Open Access Journals (Sweden)

    Eduardo Pimenta

    2007-10-01

    Full Text Available Hipertensão arterial refratária (HAR pode ser definida como persistência de níveis pressóricos elevados apesar do uso de, pelo menos, três fármacos anti-hipertensivos em doses eficazes, sempre incluindo um diurético. Obesidade, idade avançada e maior uso de algumas substâncias parecem estar relacionados com o aumento da prevalência da HAR. A avaliação desses pacientes deve ser direcionada para a identificação de fatores que contribuem para manter a HAR e possíveis causas secundárias. Atualmente, o hiperaldosteronismo é reconhecido como o fator secundário mais comum para a HAR, de modo que todos os pacientes com HAR devem ser submetidos à avaliação com o intuito de afastar a possibilidade de hiperaldosteronismo, principalmente na avaliação da relação aldosterona/renina, mesmo se os níveis sanguíneos de potássio estiverem normais. O tratamento inclui a eliminação de possíveis fatores causais, tratamento otimizado das causas secundárias, e o uso de eficazes estratégias com vários fármacos. Estudos mais recentes têm evidenciado a importância terapêutica da espironolactona, principalmente quando adicionada aos esquemas tradicionais de tratamento por provocar significantes quedas nos níveis pressóricos dos pacientes com HAR.

  10. Subjetividade, trabalho e economia solidária

    Directory of Open Access Journals (Sweden)

    Marília Veríssimo Veronese

    2012-12-01

    Full Text Available O artigo relaciona os processos de subjetivação, entendidos como processos sempre relacionais e geradores de formas de sociabilidade diversas, com o trabalho na economia solidária. Inicialmente, discute-se a articulação teórica entre subjetividade e atividade laboral. Em seguida, a dificuldade de construir a autogestão baseada na autoridade compartilhada é problematizada, com base em dados empíricos recentemente coletados junto a empreendimentos econômicos solidários (EES de diversos segmentos e portes, no estado do Rio Grande do Sul, Brasil.

  11. Enterprise Development with Flex Best Practices for RIA Developers

    CERN Document Server

    Fain, Yakov; Tartakovsky, Anatole

    2010-01-01

    If you want to use Adobe Flex to build production-quality Rich Internet Applications for the enterprise, this groundbreaking book shows you exactly what's required. You'll learn efficient techniques and best practices, and compare several frameworks and tools available for RIA development -- well beyond anything you'll find in Flex tutorials and product documentation. Through many practical examples, the authors impart their considerable experience to help you overcome challenges during your project's life cycle. Enterprise Development with Flex also suggests proper tools and methodologies,

  12. Hegel e a história mundial

    Directory of Open Access Journals (Sweden)

    Vieira, Leonardo Alves

    2006-01-01

    Full Text Available Neste estudo, a história mundial, tal como concebida por Hegel, é analisada a partir dos §§ 330-360 da Filosofia do Direito. Em seguida, procura-se coordenar estes parágrafos com as teses de Kant sobre a guerra e a paz. Finalmente, as abordagens de Kant e de Hegel são retomadas à luz do estudo de Hobsbawm sobre o cenário político internacional nos séculos 20 e 21

  13. PAULINA CHIZIANE E A HISTÓRIA DA POLIGAMIA

    OpenAIRE

    Oliveira, Jurema

    2014-01-01

    Paulina Chiziane é autora de A balada de amor ao vento (1990), Ventos do apocalipse (1995), O sétimo juramento (1999) e Niketche: uma história da poligamia (2002). Estas duas últimas obras criticam os costumes e a postura patriarcal da sociedade moçambicana, e também a prática de se obter o poder a qualquer preço. Estas obras distintas têm em comum a denúncia dos tortuosos meios encontrados por um sistema social que silencia as vozes femininas em prol de uma valoração das ações e feitos mascu...

  14. Neutronics Assessments for a RIA Fragmentation Line Beam Dump Concept

    CERN Document Server

    Boles, Jason; Reyes, Susana; Stein, Werner

    2005-01-01

    Heavy ion and radiation transport calculations are in progress for conceptual beam dump designs for the fragmentation line of the proposed Rare Isotope Accelerator (RIA). Using the computer code PHITS, a preliminary design of a motor-driven rotating wheel beam dump and adjacent downstream multipole has been modeled. Selected results of these calculations are given, including neutron and proton flux in the wheel, absorbed dose and displacements per atom in the hub materials, and heating from prompt radiation and from decay heat in the multipole.

  15. História e historiografia na Antiguidade Oriental

    OpenAIRE

    Carreira, José Nunes

    1982-01-01

    De há cento e cinquenta anos a esta parte vive-se uma espécie de segundo Renascimento. Não será efectivamente exagero nem abuso terminológico apelidar de «Renascimento oriental» a profunda transformação dos conhecimentos e o espantoso enriquecimento das vivências culturais resultantes da descoberta das literaturas, das religiões, das ciências e da arte do Próximo Oriente antigo. E ainda nos encontramos mais propriamente na fase preparatória do «Humanismo», sob o alvoroço da descoberta. Que se...

  16. Polirradiculoneurite e malária: relato de um caso

    Directory of Open Access Journals (Sweden)

    Carlos A. M. Guerreiro

    1985-06-01

    Full Text Available Registro de caso de paciente que três semanas após malária por Plasmodium falciparum desenvolveu forma severa de polirradiculoneurite, com tetraplegia e comprometimento de vários nervos cranianos (VI, VII, IX, X, evoluindo para a morte. A síndrome de Guillain-Barré tem sido considerada desordem imune relacionada a diversos estímulos antigênicos desencadeantes. O caso registrado sugere que protozoários podem ser um desses fatores antigênicos.

  17. Schwannoma da goteira olfatória: relato de caso

    OpenAIRE

    Souza Heider Lopes de; Ramos Ana Maria de Oliveira; Ramos Carlos Cesar Formiga; Melo Syomara Pereira da Costa; Pereira Hougelle Simplício Gomes; Madureira João Flávio Gurjão; Lana Janaína Martins de

    2003-01-01

    Schwannomas intracranianos não associados a nervos cranianos são incomuns e raramente encontrados na região subfrontal. Apresentamos raro caso de schwannoma da goteira olfatória, acometendo paciente de 27 anos, masculino, com quadro iniciado há 1 ano com perda da olfação e cefaléia. Ao exame de admissão, apresentava papiledema bilateral e anosmia. Tomografia computadorizada de Cranio (TC) revelou processo expansivo bifrontal hipodenso ao parênquima, com aspecto multicístico, sem captação do c...

  18. Fotografia, Memória e Arte. Sobras de Geraldo de Barros

    OpenAIRE

    Pereira Gonçalves, Sandra Maria Lúcia

    2011-01-01

    O presente artigo reflete acerca das relações que se estabelecem entre a fotografia e a memória, e no caso específico da série fotográfica Sobras, de Geraldo de Barros, que inspira este trabalho, a transformação dessa matéria (fotografia e memória) em objeto artístico. Três são os eixos temáticos trabalhados: fotografia, memória e arte. Para refletir acerca da relação entre memória e fotografia a perspectiva teórica é bergsoniana. A relação entre percepção e memória será abordada e a partir d...

  19. Oralidade e memória: o registro audiovisual de A história de Lélio e Lina de Guimarães Rosa

    Directory of Open Access Journals (Sweden)

    Eleni Ferreira de Souza Gonçalves

    2016-09-01

    Full Text Available Este trabalho aborda as questões da memória e da oralidade na narrativa de Guimarães Rosa. Adotamos uma análise comparativa entre a novela A história de Lélio e Lina e o respectivo registro oral – em forma de documentário – feito pelos Grupos de Contadores de Histórias de Cordisburgo e do Morro da Garça, em Minas Gerais. Analisamos no texto roseano transposto para o suporte audiovisual o que se mantém em um e outro e verificamos os possíveis efeitos que causam ao leitor e ao espectador. Na transposição, se a memória do texto escrito permanece, os elementos da oralidade e da memória sertaneja adquirem outra dimensão na fala dos narradores. Referenciamo-nos no livro A memória Coletiva, de Maurice Halbwachs, e na entrevista de Guimarães Rosa a Gunter Lorenz, na qual o escritor enfatiza aspectos decisivos de sua escrita, sua relação com a língua e linguagem e sua memória em relação ao sertão.

  20. Memória, história e narrativa: Os desafios da escrita biográfica no contexto da luta nacionalista em Moçambique

    Directory of Open Access Journals (Sweden)

    Teresa Cruz e Silva

    2015-04-01

    Full Text Available Tomando como ponto de partida a inter‑relação entre História, memória e narrativa, com este texto trazemos para discussão questões e interrogações de carácter metodológico sobre o uso da narrativa biográfica como possibilidade de História. As nossas reflexões são realizadas a partir de várias leituras, feitas a posteriori, sobre os percursos de vida de Zedequias Manganhela e Eduardo Mondlane. Procuramos, assim, situar a configuração e reconfiguração dos discursos no contexto da “recuperação” de uma história nacional, que também mergulha no seio da construção de figuras de heróis nacionais. Tudo isto se situa ainda, quer no quadro de uma história oficial de Moçambique, quer no quadro da própria história institucional da Igreja Presbiteriana de Moçambique/Missão Suíça à qual os dois protagonistas se encontravam intrinsecamente ligados, desafiando-nos a enfrentar as armadilhas da escrita biográfica.

  1. Contracultura e contramemória em Os Subterrâneos, de Jack Kerouac.

    OpenAIRE

    Silva Junior, Sávio Augusto Lopes da

    2014-01-01

    Programa de Pós-Graduação em Letras. Departamento de Letras, Instituto de Ciências Humanas e Sociais, Universidade Federal de Ouro Preto. Este trabalho pretende analisar a obra literária Os subterrâneos, do autor norte-americano Jack Kerouac, tendo como base os termos contramemória e contracultura. A expressão contramemória foi cunhada por Aleida Assmann (2011), que observa a forma como a literatura constrói uma memória formada a partir de descartes dos arquivos da cultura oficial. Estes d...

  2. Os canais de transmissão da política monetária

    OpenAIRE

    José Alberto Fuinhas

    2002-01-01

    Com o presente artigo procurou-se fazer uma súmula dos principais canais de transmissão da política monetária. São discutidas as implicações da presença dos canais de transmissão na condução da política monetária pelo banco central. Palavras chave: Canais de transmissão monetária, política monetária.

  3. Promoção do crescimento de alface por rizobactérias

    OpenAIRE

    S.S. Freitas; A. M. T. Melo; V. P. Donzeli

    2003-01-01

    Rizobactérias promotoras do crescimento de plantas (RPCPs) podem ser uma alternativa para o aumento da produtividade de várias espécies vegetais, inclusive alface. Conduziram-se quatro experimentos com isolados de rizobactérias de diversas origens para verificar sua capacidade de promoção de crescimento de plantas de alface. Ao todo, testaram-se 77 isolados de Pseudomonas do grupo fluorescente, 23 de Bacillus e 11 de outras bactérias rizosféricas em areia esterilizada, em solo esterilizado, e...

  4. Os canais de transmissão da política monetária

    OpenAIRE

    José Alberto Fuinhas

    2002-01-01

    Com o presente artigo procurou-se fazer uma súmula dos principais canais de transmissão da política monetária. São discutidas as implicações da presença dos canais de transmissão na condução da política monetária pelo banco central. Palavras chave: Canais de transmissão monetária, política monetária.

  5. Linfoadenopatia transitória secundária à síndrome nefrótica

    Directory of Open Access Journals (Sweden)

    Olívia Meira Dias

    2012-05-01

    Full Text Available Linfadenomegalias mediastinais secundárias a hipervolemia são achados tomográficos subdiagnosticados. Descrevemos neste paciente com função cardíaca normal, achados de congestão pulmonar associados a alargamento dos linfonodos mediastinais. A síndrome nefrótica causando hipoalbuminemia, baixa pressão oncótica plasmática e aumento da pressão transcapilar foi a causa provável dos achados radiológicos.

  6. Republicanismo neo-romano e democracia contestatória

    Directory of Open Access Journals (Sweden)

    Ricardo Silva

    2011-06-01

    Full Text Available O artigo examina o conceito de democracia derivado da fórmula "neo-romana" da liberdade como não-dominação. Para os autores vinculados à tradição neo-romana, a realização do princípio republicano do povo como "guardião da liberdade" depende da constituição de um modelo de democracia contestatória, apresentado como superação dos modelos da democracia eleitoral e da democracia participativa - ambos considerados tributários de uma concepção positiva de liberdade. Após deter-se no exame do conceito de democracia contestatória, o artigo analisa as principais críticas a esse conceito. Duas objeções recebem destaque: a de que o racionalismo do modelo contestatório conduz à despolitização da deliberação democrática e a de que o excessivo zelo dos neo-romanos contra o populismo e a tirania da maioria reproduz os traços "elitistas" da tradição republicana clássica. Conclui-se com uma breve avaliação da pertinência dessas críticas.

  7. SIMPLICIDADE VOLUNTÁRIA: ESCOLHENDO UMA NOVA FORMA DE VIVER

    Directory of Open Access Journals (Sweden)

    Luis Fernando Hor-Meyll

    2016-08-01

    Full Text Available O estudo investigou motivações de consumidores para adotar a simplicidade voluntária e os impactos causados em suas vidas. Durante dez meses, foram acompanhados os debates de um grupo sobre o tema no Facebook e conduzidas treze entrevistas em profundidade com participantes do grupo. Através de análise de conteúdo, surgiram evidências de que as principais motivações para a adoção da simplicidade voluntária são de natureza pessoal, levando a mudanças que afetam ocupação profissional, moradia, transporte, alimentação, consumo e descarte de bens, organização pessoal e consciências ecológica e social. Diversos benefícios da adoção da vida simples foram apontados, além de algumas consequências negativas para a vida dos adotantes. São oferecidas proposições decorrentes do estudo para orientar pesquisas futuras.http://dx.doi.org/10.21714/pretexto.v17i2.3772

  8. Fontes binárias supermoles de raios X

    Science.gov (United States)

    Pires, A. M.; Janot Pacheco, E.

    2003-08-01

    Estuda-se as características físicas das fontes supermoles (de raios X (SSS), utilizando dados ópticos e em altas energias, no âmbito de um trabalho de IC. Trata-se de binárias que apresentam espectro X muito mole, baixas temperaturas e altas luminosidades bolométricas. Esse sistemas são compostos por uma anã branca realizando fusão em sua superfície, a partir de matéria perdida pela estrela companheira. Os resíduos de fusão se acumulam na superfície da anã branca, e essa pode ultrapassar o limite de Chadrasekhar, produzir um colapso gravitacional, sendo esse um dos cenários propostos para as explosões de SN Ia. Apresentamos nesta comunicação o estado da arte das características físicas das fontes SSS, situando-as no âmbito das VCs. Procuramos também situar esses objetos em relação às variáveis galácticas V Sge, na medida em que os dois grupos apresentam certas caracerísticas bastante semelhantes.A metodologia adotada é aquela pedagógico-cognitiva clássica de um trabalho de IC na área de ciências exatas.

  9. Complexidade e as Redes de Economia Solidária

    Directory of Open Access Journals (Sweden)

    Luís Miguel Luiz dos Santos

    2013-03-01

    Full Text Available O objetivo primordial da elaboração deste ensaio é demonstrar como as redes de economia solidária, que emergem como um contramovimento à hegemonia individualista capitalista, podem se constituir num modelo socioeconômico mais factível e sustentável. Parte-se, através do pensamento complexo de Edgar Morin, do pressuposto que a crise social hoje vivenciada é, sobretudo, uma crise no modo como o conhecimento foi concebido na sociedade moderna. Nesse contexto, argumenta-se que a economia solidária constitui-se numa forma de produção e sociabilidade mais condizente com os desafios do mundo atual, considerando as contradições e desequilíbrios socioambientais crescentes, em que os modelos organizacionais convencionais apoiados no isolamento das partes, na hiperespecialização e na fragmentação e isolamento dos saberes, demonstram-se incapazes de superar as incoerências e ameaças à própria sustentabilidade sistêmica.

  10. Redes sociais, perfil empreendedor e trajetórias

    Directory of Open Access Journals (Sweden)

    Victor Silva Corrêa

    2014-03-01

    Full Text Available Neste artigo, enfoca-se a trajetória dos empreendedores oriundos de camadas de baixa renda, que se projetaram no mundo empresarial. Sua base teórica advém da literatura sobre as redes sociais e o embeddedness, associada àquela sobre os atributos empreendedores. As evidências empíricas fazem parte do resultado de uma pesquisa qualitativa exploratória, derivada dos estudos de caso múltiplos holísticos, realizados com dois empreendedores localizados no município de Belo Horizonte, em Minas Gerais, Brasil. A metodologia contou com os recursos provenientes da sociometria longitudinal, o que tornou possível a identificação e a visualização do padrão de evolução das redes, além da verificação de seus momentos principais de transição. Os resultados evidenciam que o desenvolvimento dos empreendedores deve-se a uma interação positiva entre os recursos disponíveis nas redes sociais, que eles conseguiram acessar e alavancar ao longo do tempo, e, ao mesmo tempo, a presença de certos atributos pessoais.

  11. Recent results on the RIA test in IGR reactor

    Energy Technology Data Exchange (ETDEWEB)

    Asmolov, V.; Yegorova, L. [Nuclear Safety Institute, Moscow (Russian Federation)

    1997-01-01

    At the 23d WRSM meeting the data base characterizing results of VVER high burnup fuel rods tests under reactivity-initiated accident (RIA) conditions was presented. Comparison of PWR and VVER failure thresholds was given also. Additional analysis of the obtained results was being carried out during 1996. The results of analysis show that the two different failure mechanisms were observed for PWR and VVER fuel rods. Some factors which can be as the possible reasons of these differences are presented. First of them is the state of preirradiated cladding. Published test data for PWR high burnup fuel rods demonstrated that the PWR high burnup fuel rods failed at the RIA test are characterized by very high level of oxidation and hydriding for the claddings. Corresponding researches were performed at Institute of Atomic Reactors (RLAR, Dimitrovgrad, Russia) for large set of VVER high burnup fuel rods. Results of these investigations show that preirradiated commercial Zr-1%Nb claddings practically keep their initial levels of oxidation and H{sub 2} concentration. Consequently the VVER preirradiated cladding must keep the high level of mechanical properties. The second reason leading to differences between failure mechanisms for two types of high burnup fuel rods can be the test conditions. Now such kind of analysis have been performed by two methods.

  12. Infinito uma história a contar

    Directory of Open Access Journals (Sweden)

    Patrícia Alexandra da Silva Ribeiro Sampaio

    2016-02-01

    Full Text Available O infinito sempre foi um tema controverso que afectou a mente humana. A sua aceitação como objecto de estudo na Matemática não foi pacífica, sendo ainda muito recente, apesar da longa história que lhe está associada. Faz-se uma pequena descrição das ideias relacionadas a este conceito desde a Grécia antiga até à Idade Média, distinguindo depois os séculos após o Renascimento devido à riqueza de descobertas que ocorreram. Salienta-se que só no século XIX é que Cantor mostrou, relativamente ao tamanho dos conjuntos, que há infinitos iguais e diferentes. As suas teorias para a teoria de conjuntos revolucionaram então a Matemática. O infinito actual finalmente tinha sido incorporado nela. Apesar de toda esta revolucionária e fabulosa teoria, aos poucos foram-se descobrindo algumas contradições, mas vários dos seus problemas foram posteriormente solucionados no século XX.

  13. O dilema extensão universitária

    Directory of Open Access Journals (Sweden)

    Dagmar Hunger

    2014-09-01

    Full Text Available Objetivou-se analisar como um grupo de professores universitários conceitua a Extensão Universitária, ou melhor, como avalia tal função no cotidiano das ações docentes. Discutiu-se o material empírico (entrevistas com base na teoria de Norbert Elias, interpretando-se as forças específicas compulsivas no jogo universitário. Constatou-se que os professores entrevistados se baseiam em grupos de intelectuais que jogam e se perdem entre as funções do ensino, da pesquisa e da extensão, e assim medem as suas forças. Ora buscam na força da pesquisa o reconhecimento acadêmico ou, então, criticam a Extensão sem envolvimento com pesquisa ou, ainda, são favoráveis à Extensão e propõem a modificação do mundo circundante por intermédio da Extensão, defendendo-a por garantir melhor formação profissional. Concluiu-se que a Extensão Universitária é um dilema acadêmico, ou melhor, evidenciam-se tentativas de resoluções, mas ainda nenhuma delas plenamente aceitável pela totalidade da comunidade acadêmica.

  14. Resultados da Intervenção Coronária Percutânea Primária de Acordo com o Tempo Total de Isquemia

    OpenAIRE

    2014-01-01

    Introdução: O tratamento do infarto agudo do miocárdio com supradesnivelamento de ST tem a intervenção coronária percutânea primária como método preferencial de reperfusão. Este estudo teve como objetivo avaliar a evolução hospitalar de pacientes com infarto agudo do miocárdio com supradesnivelamento de ST, conforme o tempo total de isquemia, até a realização de intervenção coronária percutâne...

  15. Emprego da cromatografia líquida de alta eficiência na determinação de cortisol sérico em substituição à técnica de radioimunoensaio High-performance liquid chromatography application for serum cortisol quantification as a substitute for radioimmunoassay

    Directory of Open Access Journals (Sweden)

    Eduardo Kinio Sugawara

    2008-10-01

    is routinely measured by radioimmunoassay (RIA. In the determination of free urinary cortisol this technique has been replaced by the high-performance liquid chromatography mainly in the diagnosis of Cushing syndrome. As to serum cortisol determination, there is no evidence of the application of liquid chromatography as a substitute for other analytical techniques. OBJECTIVE: The development of an analytical methodology using reversed-phase high-performance liquid chromatography (RP-HPLC to determine serum cortisol levels as a substitute for RIA in order to reduce radioactive waste. MATERIAL AND METHODS: Cortisol was directly quantified by RP-HPLC in previously ether-extracted serum samples. Triamcinolone acetonide was used as internal standard (IS. The chromatographic separation was developed in a BDS-Hypersil-C18® column (125 x 4 mm, 5 µm using water-acetonitrile (72:28; v/v as mobile phase at 1 ml/min and steroid peaks were measured at 243 nm. RESULTS: Cortisol and IS presented retention time of 3.4 and 7.1 min, respectively. The precision was less than 10% and accuracy was less than 4%. DISCUSSION: The method was effective and efficient, with good sensitivity and linearity in the concentration range of 2.5 to 60.0 µµg/dl. CONCLUSION: The present methodology substitutes RIA at clinical application.

  16. Trajetórias, narrativas e memórias de imigrantes libaneses no Ceará.

    Directory of Open Access Journals (Sweden)

    Ruben Maciel Franklin

    2015-05-01

    Full Text Available No presente artigo, utilizamos as memórias de descendentes libaneses, nascidos em Fortaleza-CE, enquanto arcabouço documental para empreendermos a discussão acerca de vínculos familiares e de conterraneidade explorados pelos imigrantes na decisão de deslocarem-se do Líbano e, em algum momento, optarem pelo Ceará em seus projetos de vida. Com isso, podemos alcançar as múltiplas movimentações em que esses sujeitos estavam envolvidos, tanto no estado como nas mais diversas cidades brasileiras, através de contatos e interações emergidas no processo imigratório. As memórias, nesse âmbito, são percorridas levando-se em conta todo o material afetivo e “nostálgico” presentes nas narrativas dos descendentes, atendo-se igualmente ao fato de que as mesmas são demarcadas por “esquecimentos” e elaboradas a partir de identidades sociais postuladas dentro das famílias.

  17. Reformas tributária e previdenciária e a economia brasileira no longo prazo

    Directory of Open Access Journals (Sweden)

    Vladimir Kühl Teles

    2006-03-01

    Full Text Available O presente artigo busca simular o efeito das reformas tributária e previdenciária sobre variáveis chave da economia brasileira no longo prazo. Para tanto, um modelo de equilíbrio geral computável de gerações superpostas sob a especificação de um modelo de crescimento endógeno à la Lucas (1988 é utilizado. Dentre os principais resultados pode-se destacar que as reformas por si só não são capazes de melhorar a performance de variáveis como taxa de juros e crescimento no longo-prazo, entretanto, ao criar oportunidades para diminuir a dívida pública e aumentar o investimento em educação, tornam-se fundamentais para uma melhoria em tais variáveis.The paper simulates the effects of tax and social security reforms on the main variables of the Brazilian Economy in the long run. For that matter a computable general equilibrium model of overlapping generations à la Lucas (1988 is employed. The main results are that the reforms in themselves are not capable of affecting the performance of the interest rate and the long run growth rate of the economy. However, as long as they make it possible to reduce the public debt and to increase the investment in education, they become important for the improvement of those variables.

  18. 3'-L-monoiodothyronine: development of a radioimmunoassay and demonstration of in vivo conversion from 3',5'-diiodothyronine. [/sup 125/I tracer technique

    Energy Technology Data Exchange (ETDEWEB)

    Smallridge, R.C.; Wartofsky, L.; Green, B.J.; Miller, F.C.; Burman, K.D.

    1979-01-01

    A sensitive, reproducible RIA for the measurement of 3'-L-monoiodothyronine (3'T/sub 1/) is described. Mean intra- and interassay coefficients of variation were 2.4% and 22.5%, respectively. Cross-reactivity with other iodothyronines was negligible, except for 3,3'-L-diiodothyronine (3,3'T/sub 2/) which started to demonstrate cross-reactivity when 3,3'T/sub 2/ levels were elevated above 35 ng/dl. Fifty percent displacement occurred when 500 pg 3,3'T/sub 2/ were added to the 3'T/sub 1/ assay. Employing this assay, 11 normal subjects and 7 pregnant women had serum 3'T/sub 1/ levels below the limits of detectability of the assay (<2.5 ng/dl), whereas 17 hyperthyroid patients had elevated levels of 3'T/sub 1/, with the mean (+-SD) values being 6.5 +- 3.0 ng/dl. Serum 3'T/sub 1/ levels were present in all cord sera measured (7.3 +- 2.3 ng/dl; n = 19), and the highest levels of 3'T/sub 1/ observed were in 38- to 40-week gestation amniotic fluid specimens (15.4 +- 8.4 ng/dl; n = 20). Compared to other iodothyronines, it seems that a relatively low proportion of 3'T/sub 1/ is bound to circulating proteins, as the mean percentage of dialyzable 3'T/sub 1/ in 12 normal subjects was 5.7 +- 1.2%. An oral dose of 3'T/sub 1/ (120 ..mu..g) given to 2 euthyroid individuals resulted in peak serum levels of 28 ng/dl 2 h after ingestion. After iv administration of 3'5'T/sub 2/ to 2 athyreotic patients (1 hypothyroid and the other euthyroid on replacement T/sub 4/), 3'T/sub 1/ levels rose from undetectable levels to 20 ng/dl. It was concluded that 3'T/sub 1/ is routinely detectable in the serum of hyperthyroid but not normal individuals, and even higher levels are present in cord sera and amniotic fluid. Moreover, the study demonstrates that in vivo conversion of 3',5'T/sub 2/ to 3'T/sub 1/ may occur.

  19. Acute effects of propylthiouracil (PTU) on thyroidal iodide organification and peripheral iodothyronine deiodination: correlation with serum PTU levels measured by radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Cooper, D.S. (Massachusetts General Hospital, Boston); Saxe, V.C.; Meskell, M.; Maloof, F.; Ridgway, E.C.

    1981-01-01

    Propylthiouracil (PTU) levels in serum have been measured by RIA in five normal volunteers and four patients with untreated Graves's disease. Peak PTU levels after 50, 200, and 300 mg PTU were 0.91 +/- 0.13, 2.9 +/- 0.6, and 4.0 +/- 0.6 ..mu..g/ml in the normal subjects and 1.04 +/- 0.1, 4.5 +/- 0.7, and 7.1 +/- 0.4 ..mu..g/ml in the hyperthyroid patients, respectively. Peak PTU levels and PTU area under the curve were significantly greater at the 300-mg dose (P < 0.01) in the hyperthyroid patients compared to the normal subjects. The effect of PTU on iodide organification was assessed by perchlorate discharge testing. Both the normals and the hyperthyroid patients had normal basal perchlorate-dischargeable /sup 123/I. Four hours after an oral dose of PTU, the hyperthyroid patients had a greater percentage of perchlorate-dischargeable /sup 123/I than the euthyroid controls (+38 +/- 5% vs. 13 +/- 2%; P < 0.02). There was a highly significant correlation between serum PTU levels and perchlorate-dischargeable iodide in both normals (r = 0.86; P < 0.001) and hyperthyroid patients (r = 0.83; P < 0.001). T/sub 3/ and rT/sub 3/ were measured serially over 24 h after single oral doses of 50, 200, and 300 mg PTU. In normal volunteers, serum T/sub 3/ declined slightly but significantly after the 200- and 300-mg doses; an increase in rT/sub 3/ was also noted, but the changes did not attain statistical significance. In the hyperthyroid patients, serum T/sub 3/ fell from 501 +/- 29 to 379 +/- 27 ng/dl after 50 mg PTU (P < 0.001), from 433 +/- 40 to 264 +/- 18 ng/dl after 200 mg PTU (P < 0.001), and from 463 +/- 40 to 335 +/- 18 ng/dl after 300 mg PTU (P < 0.05). Serum rT/sub 3/ rose after each dose: from 119 +/- 12 to 131 +/- 11 ng/dl (P = NS) after 50 mg PTU, from 113 +/- 11 to 146 +/- 12 ng/dl (P < 0.02) after 200 mg PTU,

  20. Efectos de la desnutrición sobre la colagenización de anastomosis intestinales: análisis de procolágeno y telopéptido carboxiterminal mediante radioinmunoanálisis Role of malnutrition in intestinal anastomosis collagenization: an analysis of procollagen (PINP and carboxyterminal telopeptide (ICTP by radioimmunoassay

    Directory of Open Access Journals (Sweden)

    J. M. Álamo

    2007-02-01

    Full Text Available Introducción: diversos factores influyen en la cicatrización correcta de las suturas intestinales tras la práctica de una resección intestinal. Uno de los factores más implicados es el estado nutricional del paciente. Objetivos: evaluar la influencia de la desnutrición inducida sobre la viabilidad de una anastomosis intestinal primaria mediante el análisis del procolágeno (PINP como marcador de la síntesis de colágeno I, y del telopéptido carboxiterminal del colágeno I (ICTP como marcador de la destrucción del mismo. Métodos: 40 ratas Wistar y material de radioinmunoensayo. Métodos: diseñamos 2 grupos de ratas, 20 animales por cada grupo: grupo control (A y grupo "desnutrición" (B. Se analiza PINP e ICTP mediante RIA sobre tejido colónico homogeneizado, preanastomótico y anastomótico. Resultados: existen unos niveles menores de PINP en el colon de las ratas del grupo B comparado con el colon del grupo A (0,3620 y 0,4340 µg/g respectivamente (p = 0,032. Hay un mayor nivel de ICTP analizado en el colon del grupo B (0,9545 en contraposición a 0,8460 µg/g en el grupo A (p = 0,875. En las anastomosis del grupo B existe una menor síntesis de PINP en comparación con el grupo A (0,376 y 0,468 µg/g respectivamente, p = 0,002. Conclusiones: la anastomosis colónica incrementa los niveles de PINP e ICTP en el tejido cicatricial (p = 0,000; la malnutrición reduce la colagenización de las anastomosis (p = 0,000.Introduction: some clinical, anatomo-pathological, and technical factors influence the correct healing of intestinal suture following an intestinal resection. One of the most influential factors is patient nutritional status. Objectives: to evaluate the influence of malnutrition on the viability of primary intestinal anastomosis by the analysis of collagen I deposition. Methods: 40 Wistar rats, radioimmunoassay material. We used 2 groups of rats, 20 animals in each group: a control group (A and a "malnutrition" group (B

  1. Memória e justiça: o negacionismo e a falsificação da história

    Directory of Open Access Journals (Sweden)

    Odilon Caldeira Neto

    2009-12-01

    Full Text Available O presente artigo busca analisar os embates gerados dentro do processo de formação e transformação das memórias coletivas, em especial no caso da memória das vítimas e sobreviventes da Segunda Guerra Mundial, particularmente com o Holocausto (Shoah. Procura ainda compreender como se dá o surgimento de teses que afirmam a inexistência do Holocausto e da existência de um suposto complô judaico de dominação mundial, ou seja, o fenômeno negacionista e os reflexos desta prática no Brasil, assim como os esforços de diversas categorias profissionais em salvaguardar a memória e a disciplina histórica das ameaças de falsificações intole-rantes e discriminatórias.

  2. Elementos para uma reconstrução da história universal: pensamento e memória na filosofia negativa de Hegel

    OpenAIRE

    Borges, Thiago Mendes [UNIFESP

    2013-01-01

    O propósito desta pesquisa consiste no estudo da especifidade do conceito de “história universal” nas “Lições sobre a filosofia da história universal” de Georg Wilhelm Friedrich Hegel. Propõe-se uma apresentação das interpretações do conceito presente nas obras de alguns estudiosos de Hegel, para elucidar sua reflexão da história enquanto meditação sobre as ruínas. Para isso, será de nosso interesse a exposição da idéia de “fim da história”, da memória como sinônimo de pensamen...

  3. Prevalência de dentes supranumerários na consulta de odontopediatria da Clínica Dentária Universitária Egas Moniz

    OpenAIRE

    Coimbra, Filipe Simplício Brinca Glória

    2016-01-01

    Dissertação para obtenção do grau de Mestre no Instituto Superior de Ciências da Saúde Egas Moniz Objetivo: Avaliar a prevalência de dentes supranumerários numa determinada população pediátrica da Clínica Dentária Universitária Egas Moniz, através da análise de Ortopantomografias e História Clínicas. Métodos: Foram examinadas Ortopantomografias e Histórias Clínicas de 100 indivíduos com idades compreendidas entre os 6 e os 18 anos para o estudo epidemiológico sobre a ocorrência de dente...

  4. Reforma agrária: o impossível diálogo sobre a História possível

    OpenAIRE

    José de Souza Martins

    1999-01-01

    Os desencontros entre o governo, de um lado, e o MST, a Igreja e as oposições, de outro, quanto à política de reforma agrária, só podem ser compreendidos se tivermos em conta o que vem a ser a questão agrária no Brasil. Num país em que o grande capital se tornou proprietário de terras, a concepção clássica da questão agrária, e das reformas que ela pede, fica substancialmente alterada. São essas alterações que propõem as condições e os limites da reforma agrária no país. São elas, também que ...

  5. Distonia laríngea respiratória

    Directory of Open Access Journals (Sweden)

    Lebl Mariana Dantas Aumond

    2003-01-01

    Full Text Available A distonia laríngea respiratória (DLR é uma desordem rara caracterizada por espasmos da musculatura adutora das pregas vocais durante a fase inalatória da respiração, com manifestação clínica de dispnéia e estridor. O diagnóstico etiológico do estridor laríngeo, entretanto, nem sempre é fácil de ser realizado, principalmente em situações emergenciais, de forma que a DLR pode não ser diagnosticada, o que nos leva a supor ser mais freqüente do que usualmente é descrita. O diagnóstico da DRL requer primeiramente a realização de uma história médica e exames laringológico e neurológico apropriados, com ênfase na verificação da presença de características distônicas e na exclusão de outras etiologias causadoras de movimentos paradoxais de pregas vocais. Muitos tratamentos foram propostos para a DLR, mas nenhum deles apresentou resultados satisfatórios. O uso da Toxina Botulínica do tipo A (Botox® no músculo tireoaritenoídeo tem oferecido melhoras admiráveis, apesar dos poucos casos descritos. Apresentamos dois casos clínicos de pacientes com DLR tratados com Botox® que apresentavam o fechamento glótico inspiratório causado tanto pelos espasmos anômalos dos músculos tireoaritenoídeos, como pela movimentação paradoxal da epiglote. Dentro da classificação proposta por Koufman e Blabock para as distonias laríngeas, inserimos um novo subtipo de DLR caracterizado pela presença de paroxismos de adução de estruturas glóticas e supraglóticas durante a respiração.

  6. Analgesia pós-operatória Postoperative analgesia

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    Betina Sílvia Beozzo Bassanezi

    2006-04-01

    Full Text Available JUSTIFICATIVAS E OBJETIVOS: A dor sempre foi uma das maiores preocupações do homem, entretanto, apesar dos progressos da ciência, ainda existem várias barreiras ao seu adequado tratamento, incluindo a falta de conhecimento por parte da equipe médica, sobre o mecanismo das diversas drogas e técnicas empregadas. O objetivo deste trabalho é abordar as principais drogas e técnicas empregadas no controle da dor pós-operatória, visando estimular o interesse sobre o assunto bem como aumentar a eficácia do tratamento dado aos pacientes. CONTEÚDO: Está ressaltada neste artigo, a importância da adequada analgesia pós-operatória, considerando as principais drogas e técnicas utilizadas no controle da dor, seus mecanismos de ação, posologias, vias de administração e efeitos colaterais, bem como a importância da integração de toda a equipe envolvida nos cuidados do paciente para o sucesso do tratamento. O tratamento inadequado da dor no pós-operatório não se justifica, pois há um arsenal considerável de drogas e técnicas analgésicas. O que se faz necessário, portanto, é que toda equipe, anestesistas, cirurgiões, e enfermeiros tenham conhecimento e estejam integrados na utilização deste arsenal.BACKGROUND AND OBJECTIVES: Pain has been one of the men's biggest worries. Despite of scientific progress there still exist many barriers in an adequate treatment of pain including the lack of knowledge of many drugs and pain management techniques. The objective of this study is to discuss the main drugs and analgesics process in an effort to stimulate our colleague interest about the subject and thus increasing treatment efficiency of our patients. CONTENTS: It is emphasized in this study the importance of an adequate postoperative analgesia discussing the main drugs and techniques used in pain management, their mechanism of action, dose, administration route and side effects of each drug. It is also pointed out the great importance

  7. RIA simulation tests using driver tube for ATF cladding

    Energy Technology Data Exchange (ETDEWEB)

    Cinbiz, Mahmut N. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Brown, N. R. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Lowden, R. R. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Linton, K. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Terrani, K. A. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

    2017-07-01

    Pellet-cladding mechanical interaction (PCMI) is a potential failure mechanism for accident-tolerant fuel (ATF) cladding candidates during a reactivity-initiated accident (RIA). This report summarizes Fiscal Year (FY) 2017 research activities that were undertaken to evaluate the PCMI-like hoop-strain-driven mechanical response of ATF cladding candidates. To achieve various RIA-like conditions, a modified-burst test (MBT) device was developed to produce different mechanical pulses. The calibration of the MBT instrument was accomplished by performing mechanical tests on unirradiated Generation-I iron-chromium-aluminum (FeCrAl) alloy samples. Shakedown tests were also conducted in both FY 2016 and FY 2017 using unirradiated hydrided ZIRLO™ tube samples. This milestone report focuses on testing of ATF materials, but the benchmark tests with hydrided ZIRLO™ tube samples are documented in a recent journal article.a For the calibration and benchmark tests, the hoop strain was monitored using strain gauges attached to the sample surface in the hoop direction. A novel digital image correlation (DIC) system composed of a single high-speed camera and an array of six mirrors was developed for the MBT instrument to better resolve the failure behavior of samples and to provide useful data for validation of high-fidelity modeling and simulation tools. The DIC system enable a 360° view of a sample’s outer surface. This feature was added to the instrument to determine the precise failure location on a sample’s surface for strain predictions. The DIC system was tested on several silicon carbide fiber/silicon carbide matrix (SiC/SiC) composite tube samples at various pressurization rates of the driver tube (which correspond to the strain rates for the samples). The hoop strains for various loading conditions were determined for the SiC/SiC composite tube samples. Future work is planned to enhance understanding of the failure behavior of the ATF cladding candidates of age

  8. Na oficina da história de Jenknis: Comentários sobre o livro "A História Repensada"

    OpenAIRE

    Ribeiro, Renilson Rosa

    2010-01-01

    O presente ensaio desenvolve uma reflexáo sobre as proposições críticas lançadas pelo historiador britânico Keith Jenkins, no recente livro lançado no Brasil A História Repensada (Sáo Paulo, contexto, 2001), sobre a disciplina História no mundo pós-moderno. 

  9. A Construção da Memória em José Saramago

    Directory of Open Access Journals (Sweden)

    Gisele Ambrósio Gomes

    2010-07-01

    Full Text Available O presente artigo tem por objetivo analisar a elaboração das memórias de infância e de adolescência do escritor José Saramago através da escrita autobiográfica encontrada em seu livro intitulado As Pequenas Memórias.

  10. A Construção da Memória em José Saramago

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    Gisele Ambrósio Gomes

    2010-07-01

    Full Text Available O presente artigo tem por objetivo analisar a elaboração das memórias de infância e de adolescência do escritor José Saramago através da escrita autobiográfica encontrada em seu livro intitulado As Pequenas Memórias.

  11. HISTÓRIA DA TECNOLOGIA NO DESENVOLVIMENTO HUMANO

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    João Victor Nogueira Okido

    2017-03-01

    Full Text Available O presente artigo tem como objetivo analisar o caminho percorrido pelo homem e o desenvolvimento da tecnologia na História. Pretende também, demonstrar os importantes aspectos que contribuíram para esse processo histórico. No decorrer do processo de desenvolvimento, a capacidade de criar materiais para aprimorar as suas limitações, gera alterações em sua estrutura física e intelectual. Neste estudo, pretendemos enfatizar que, nesse processo evolutivo, o homem desenvolve tecnologias e habilidades cada vez mais avançadas. Para isso, analisaremos essa evolução nas gerações do século XX a partir de 1940 e século XXI até 2010, do autor Daniel Portillo Serrano.

  12. Uma releitura cristã da história

    OpenAIRE

    2014-01-01

    Resumo: O presente trabalho é uma análise da Historia Adversus Paganos escrita pelo presbítero hispano Orósio, que viveu a princípios do século V, a partir do ponto de vista da História da Historiografia. Buscamos, como o objetivo principal do trabalho, entender como a Teoria dos Quatro Impérios influenciou a visão de mundo de tal historiador. Utilizamos em nossa análise a definição de Antiguidade Tardia proposta por Renan Frighetto de que este período é marcado pela reformulação e pela reade...

  13. A economia solidária: Um movimento internacional

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    Jean-Louis Laville

    2012-12-01

    Full Text Available O artigo mostra como em diferentes contextos nacionais e continentais se gerou um movimento de economia solidária. A diversidade de práticas no seio da sociedade civil local e internacional merece ser sublinhada. Esta geração de iniciativas, simultaneamente políticas e econômicas surgidas nas últimas décadas, prolonga e renova a economia social, oferecendo, assim, propostas concretas para uma outra economia, num período de crise capitalista. Como tal, não pode ser ignorada na busca de um modelo económico e de uma acção pública renovada.

  14. Outros nomes, histórias cruzadas: apresentando o debate

    OpenAIRE

    Pina-Cabral, João de

    2013-01-01

    Em Grande Sertão: Veredas, João Guimarães Rosa mostra-nos como, nos nomes pessoais, a língua portuguesa é parte da constituição de cada um de nós (2001 [1956]). Nos nomes dos jagunços cujos traços o autor segue pelas veredas desse sertão, nas pessoas com quem eles se cruzam, assistimos à forma como a emoção constrói pessoas usando nomes, através de um processo dialogal de criação identitária em fluxo. A obra gira em torno a uma tragédia – a tragédia de um amor com três nomes. No jogo de ident...

  15. Com fer lectors de poesia en secundària

    OpenAIRE

    Ballester Marco, Anna

    2015-01-01

    En aquest article es presenten una sèrie d’activitats pràctiques i de reflexions amb el propòsit de fer lectors de poesia a Secundària, tot un repte per al professorat. S’hi insisteix en la necessitat de treballar a l’aula textos poètics per descobrir una altra forma d'utilitzar el llenguatge, vinculada al joc del ritme i la rima de les paraules, i perquè la poesia és indispensable per al desenvolupament intel·lectual i afectiu. Es pretén així facilitar experiències gratificants per ajudar el...

  16. DESIGN IMPROVEMENT OF THE RIA 80.5 MHZ RFQ

    CERN Document Server

    Zhao, Q; Doleans, M; Gorelov, D; Grimm, T L; Hartung, W; Marti, F; Schriber, Stanley O; Wu, X; York, R C

    2004-01-01

    An 80.5 MHz, continuous-wave, normal-conducting, radio-frequency quadrupole (RFQ) was designed for the front end of the Rare Isotope Accelerator (RIA) driver linac. It will accelerate various ion beams (hydrogen up to uranium) from 12 keV/u to about 300 keV/u. The 4-meter-long RFQ accepts the pre-bunched beam from the low energy beam transport (LEBT) and captures more than 80% with a current of ~0.3 mA. Beam dynamics simulations show that the longitudinal output emittance is small for both single- and two-charge-state ion beams with an external multi-harmonic buncher. A 4-vane resonator with magnetic coupling windows was employed in the cavity design to provide large mode separation, high shunt impedance, and a small transverse dimension. The results of beam dynamics as well as the electromagnetic simulations are presented.

  17. A radiologia dentária na gravidez

    OpenAIRE

    Silva, José Oliveira da

    2013-01-01

    Aborda-se a biossegurança aplicada a radiologia dentária relativa à radiação ionizante e a exposição aos agentes biológicos, a que pacientes, grávidas / feto e profissionais são submetidos durante os procedimentos radiológicos. As diferentes doses de radiação emitidas, pelos equipamentos utilizados em radiologia oral, quer nos sistemas analógicos, como digitais. As diversas formas de propagação de micro-organismos, durante a obtenção de imagens radiográficas. As medidas de biossegurança, para...

  18. A radiologia dentária na gravidez

    OpenAIRE

    Silva, José Oliveira da

    2013-01-01

    Aborda-se a biossegurança aplicada a radiologia dentária relativa à radiação ionizante e a exposição aos agentes biológicos, a que pacientes, grávidas / feto e profissionais são submetidos durante os procedimentos radiológicos. As diferentes doses de radiação emitidas, pelos equipamentos utilizados em radiologia oral, quer nos sistemas analógicos, como digitais. As diversas formas de propagação de micro-organismos, durante a obtenção de imagens radiográficas. As medidas de biossegurança, para...

  19. Nacionalismos e reforma agrária nos anos 50

    Directory of Open Access Journals (Sweden)

    Moreira Vânia Maria Losada

    1998-01-01

    Full Text Available O artigo visa recuperar os elementos fundamentais do ideário nacionalista durante a década de 1950, destacando a centralidade do conceito de desenvolvimento auto-sustentado. A diversidade política e ideológica do movimento nacionalista é problematizada através da análise de duas correntes preponderantes do período em foco: o nacional-desenvolvimentismo, de caráter liberal, e o nacionalismo econômico, isto é, a vertente defendida pelas esquerdas. Finalmente, é focalizado a importância da reforma agrária dentro do projeto social defendido sobretudo pelos nacionalistas econômicos, enquanto fator indispensável a uma industrialização com cidadania.

  20. Campus no Nordeste: Reforma Universitária de 1968

    OpenAIRE

    Magda Maria de Souza Campêlo Macêdo

    2012-01-01

    Análise das realizações urbano-arquitetônicas das universidades federais do Ceará (UFC), da Paraíba (UFPB), do Rio Grande do Norte (UFRN), de Alagoas (UFAL), Maranhão (UFMA), de Sergipe (UFS) e do Piauí (UFPI), executadas a partir da Reforma Universitária de 1968, com o objetivo de demonstrar que esses espaços universitários, em sua reformulação ou constituição física, obedecendo ao projeto reformista, adotaram o padrão de campus norte-americano e produziram no Nordeste configurações urbaníst...

  1. História da ciência

    OpenAIRE

    2007-01-01

    Dissertação (mestrado) - Universidade Federal de Santa Catarina, Programa de Pós-Graduação em Educação Científica e Tecnológica. No presente trabalho apresento os resultados obtidos na investigação da História da Ciência veiculada nas duas coleções de livros didáticos de ciência do ensino fundamental de 5ª a 8ª série, mais usadas nas escolas que compõem a 16ª Gerência de Educação do Estado de Santa Catarina. Para realização da investigação utilizei uma ficha composta por sete tópicos, subd...

  2. Envelhecimento, trajetórias e homossexualidade feminina

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    Andrea Moraes Alves

    2010-12-01

    Full Text Available A literatura das ciências sociais sobre o tema da homossexualidade feminina tem crescido recentemente no Brasil, mostrando o interesse despertado pelo assunto. A partir dos anos 1990, trabalhos acadêmicos discutem a homossexualidade feminina: seu significado e impacto sobre as questões de gênero, sua relação com os movimentos sociais vinculados às reivindicações sobre direitos sexuais e reprodutivos no Brasil. Grande parte desses trabalhos concentra-se sobre uma faixa etária jovem, em torno dos 20 anos de idade, e outros abordam mulheres um pouco mais velhas, entre 30 e 40 anos. No entanto, nota-se a ausência de trabalhos que discutam a homossexualidade vivida por mulheres idosas. Este artigo pretende começar a preencher essa lacuna e traz à discussão o olhar das lésbicas mais velhas e suas percepções sobre o que significa a homossexualidade feminina e o lugar que ela ocupa na trajetória de vida dessas mulheres.The social science's literature about female homosexuality has recently grown in Brazil, showing the awakened interest in this issue. Since the 1990's, academic works have discussed female homosexuality: its meanings and its impact on gender issues, its relationships with social movements, specially the ones concerned with sexual rights in Brazil. Great part of these works focus on a young age rate, and some of them are dedicated to middle age women. However, there aren't works concerned with old age women and lesbianity. This article starts to fill this gap and takes into account old age lesbians and their perceptions about homosexuality and its place in their life trajectories.

  3. Histórias por um fio: narração mediada em tempo real

    OpenAIRE

    Maduro,Daniela Côrtes

    2016-01-01

    RESUMO O ato milenar de contar histórias tem vindo a permear o meio digital, graças à adoção do computador como um instrumento de expressão artística. As "histórias por um fio" são histórias cuja integridade é de alguma forma ameaçada pelas condições em que é transmitida. Nestas histórias existe uma exploração estética das propriedades ou limitações do meio. O presente artigo pretende ser uma reflexão sobre a influência deste na transmissão de uma história, tornando evidente a ligação entre d...

  4. Histórias por um fio: narração mediada em tempo real

    OpenAIRE

    Maduro,Daniela Côrtes

    2016-01-01

    O ato milenar de contar histórias tem vindo a permear o meio digital, graças à adoção do computador como um instrumento de expressão artística. As “histórias por um fio” são histórias cuja integridade é de alguma forma ameaçada pelas condições em que é transmitida. Nestas histórias existe uma exploração estética das propriedades ou limitações do meio. O presente artigo pretende ser uma reflexão sobre a influência deste na transmissão de uma história, tornando evidente a ligação entre diversas...

  5. Histórico e os limites da reforma agrária na contemporaneidade brasileira

    OpenAIRE

    Paulo Nakatani; Rogério Naques Faleiros; Neide César Vargas

    2012-01-01

    Objetiva-se reconstituir a trajetória da questão fundiária (e agrária) na experiência de maior concentração de riqueza e recursos vivenciada no âmbito do sistema capitalista, partindo-se da premissa de que a crescente desigualdade do modelo brasileiro passa necessariamente pela questão agrária. Num segundo momento, procede-se à análise dos limites atualmente colocados a qualquer possibilidade de uma Reforma Agrária ampla e estrutural, dados pelo avanço do agronegócio nos últimos dez anos, pel...

  6. Histórias divergentes na intelectualidade docente: trajetórias formativas nas memórias de professoras do ensino municipal de São Paulo (1964-1985

    Directory of Open Access Journals (Sweden)

    Helenice Ciampi

    2017-07-01

    Full Text Available Com base na metodologia da história oral temática,analisa-se no artigo uma entrevista simultânea, na qual três docentes relatam suas trajetórias familiares, escolares e acadêmicas. Analisa-se o processo de sua formação como professoras, a partir do final dos anos 1960, e as posições políticasque assumiram no presente,2012, em relação à educação e à escola municipal paulistana no período da ditadura civil-militar no Brasil (1964-1985. O principal objetivo é mostrar que suas memórias expressam divergências individuais e coletivas e que existem outras formas de expressão da intelectualidade docente, as quais emergem das tensões econtradiçõesvivenciadas em suas trajetórias formativas e não das prescriçõespolíticas e acadêmicas educacionaisque silenciamoutras vozes, memórias e saberes.

  7. Turismo, História, Memórias e Imaginários dos Tempos da PANAIR

    Directory of Open Access Journals (Sweden)

    Karla Estelita Godoy

    2014-07-01

    Full Text Available O presente texto tem como objeto o caso da Panair do Brasil S.A., uma das companhias aéreas pioneiras no país, que dominou o setor da aviação brasileira entre 1930 e 1965. Para além da sua relevância quanto à história dos transportes aéreos e à realização de viagens turísticas, está o controverso encerramento de suas atividades, visto como perseguição política. Nesse bojo, surgem desdobramentos sociais e políticos, que culminam na criação da Família Panair, na investigação hoje feita pela Comissão Nacional da Verdade e na formação de coleções documentais sobre a Panair, em arquivos (pessoais e institucionais e em certos museus. Assim, o artigo tem como objetivos expor o estado da arte da pesquisa, traçar os primeiros horizontes teóricos e metodológicos, apresentar os estudos históricos e turísticos a respeito do tema, e realizar abordagem sobre os quadros sociais da memória, que envolvem a Família Panair. Discute a relação memória / esquecimento, verdade / imaginário, e aponta não somente o museu como lugar de memória, mas também a própria Família Panair, sendo os sujeitos que a compõem vistos como suportes das lembranças construídas coletivamente. Por fim, o texto apresenta questionamentos, que fomentam discussões relevantes acerca do tema.  Tourism, History, Memory and Imaginary: Panair Old Times - This paper aims to analyses the case of Panair do Brasil S.A., one of the pioneer airlines in the country. Panair dominated the Brazilian aviation sector between 1930 and 1965. Apart from its bearing on the history of air transport and the achievement of tours, the paper analyses the controversial closure of its activities, seen as political persecution. In this bulge, social and political consequences arise, which culminate in the creation of the Family Panair, in the recent inquiry by the National Commission of Truth and in the formation documentary collections about Panair (personal and

  8. Uma autobiografia operária: a memória entre a entrevista e o romance

    Directory of Open Access Journals (Sweden)

    J. Sergio Leite Lopes

    1999-12-01

    Full Text Available TRATA-SE da apresentação e análise de uma rara autobiografia romanceada feita por um trabalhador e entregue aos pesquisadores após entrevista realizada com o autor em trabalho de campo na vila operária da cidade de Paulista, em Pernambuco. Apesar de apresentar metáforas e fábulas comuns ao gênero de literatura para leitura em voz alta, como o cordel, o autor domina uma narrativa elaborada com três versões de uma história comum, vista por três personagens, com pequenos mistérios e surpresas: só no meio da narrativa é que o leitor percebe se tratar de uma autobiografia. Por meio de uma narrativa ficcional de sua história vivida, o autor aborda suas origens rurais, a importância de alguns membros do grupo familiar na sua infância e adolescência; aspectos não-revelados nas entrevistas que versavam sobre sua participação recente no trabalho industrial. A experiência de migração para os centros metropolitanos, a diversidade de trabalhos na indústria, a relação com a leitura e a autodidaxia desse filho caçula são fatores, como em outras autobiografias operárias de que se tem conhecimento em escala internacional, para explicar esse trabalho reflexivo por escrito sobre o passado e suas origens sociais.IN THIS article is presented and analysed a rare ficional worker's autobiography given to the anthropologists after interviews in fieldwork done in the company-town located at the city of Paulista, Pernambuco. In spite of the use of fables and metaphors that are common to the literary genre for loud voice lecture, as cordel, the author masters an elaborate narrative with three versions of the same story, seen by three characters, with some mysteries and surprises: only in the middle of the text the reader perceives that it is an autobiography. Through fictional narrative of his living history, the author describes his rural origins and the importance of some members of his family unit in his childhood and youth; all non

  9. Infecção urinária comunitária: etiologia segundo idade e sexo

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    Denise Swei Lo

    2013-06-01

    Full Text Available INTRODUÇÃO: A escolha do antimicrobiano para tratamento inicial de infecção de trato urinário (ITU costuma ser empírica e deve considerar a prevalência dos uropatógenos nas diversas faixas etárias e sexo. OBJETIVO: Avaliar a prevalência de uropatógenos em ITU comunitária e sua relação com idade e sexo. MÉTODOS: Estudo transversal conduzido em pronto socorro (PS de hospital geral, de janeiro a dezembro, 2010, em pacientes menores de 15 anos com suspeita clínica de ITU, que colheram urocultura quantitativa. Definida ITU como urocultura com crescimento de agente único > 100.000 unidades formadoras de colônia (ufc/mL na coleta por jato médio ou > 50.000 ufc/mL na coleta por sondagem vesical. RESULTADOS: Ocorreram 63.464 atendimentos no PS. Foram obtidas 2.577 uroculturas; destas, 291 foram positivas para ITU (prevalência = 11,3% das suspeitas clínicas e 0,46% dos atendimentos; 212 casos (72,8% em meninas, mediana de idade = 2,6 anos. O uropatógeno predominante foi E.coli (76,6%, seguido por Proteus mirabilis (10,3% e Staphylococcus saprophyticus (4,1%. Em lactentes 10 anos (24,4% x 0,4%; OR = 79,265; p 10 anos e de Proteus mirabilis em meninos.

  10. Memória coletiva e espacialidade étnica

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    Mohammed Elhaji

    2008-11-01

    Full Text Available O presente trabalho analisa o conceito de espacialidade (o espaço vivido, organizado e representado enquanto instância de produção de subjetividade e de enunciação e/ ou reterritorialização da identidade do grupo étnico. A partir da evidente relação entre as transformações sociais e os modos de concepção e elaboração do espaço, tentamos estabelecer um paralelo entre os traços culturais de um grupo humano dado e suas estruturas espaciais. Esboçamos, neste sentido, a nossa tese de "pregnância da memória espacial", que determinaria as inclinações geométricas e psicológicas do grupo étnico, no seu projeto biopolí­tico de produção de territórios existenciais e de organização de espaços sociais e culturais. Pretendemos comprovar, assim, que as modalidades étnicas de construção da espacialidade são processos "psico-homotéticos", cujo objetivo é a reativação de panos da subjetividade relacionados a momentos históricos ou mí­ticos contidos na memória espacial do grupo. Palavras-chave espacialidade, territorialidade, subjetividade, identidade. Abstract This paper analyzes the concept of spatiality (as a lived, organized and represented space as an instance of subjectivity production and of enunciation and/or reterritorialization of the identity of an ethnic group. Starting from the evident relationship between the social transformations and the ways of conception and elaboration of the space, we try to set a parallel between the cultural traits of a given human group and their space structures. We sketch, in this sense, our thesis of "pregnancy of the spatial memory" which might determine the geometrical and psychological trends of an ethnic group within its bio-political project of production of existential territories and of organization of social and cultural spaces. Thus we intend to prove that ethnic modalities of construction of the spatiality are "psycho-homothetic" processes, the

  11. História e literatura: uma relação de amor e ode em História do Brasil de Murilo Mendes

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    Valmir de Souza

    2008-06-01

    Full Text Available O artigo trata das relações da história com a literatura, estudo esse que aponta para a inter-relação de campos culturais distintos, mas que coloca também novas abordagens do fenômeno histórico. A literatura pode recontar a história estabelecida sob a ótica do autor satírico, fazendo com que através do riso se possam compreender os processos históricos, e dessacralizando versões cristalizadas da historiografia oficial. É nesse sentido que o diálogo entre literatura e história pode apontar para as tensões presentes na história e nas interpretações e representações do passado. São tomados como objeto de análise eventos da história do Brasil e as interpretações poéticas que deles faz o poeta Murilo Mendes.

  12. A ciência da história e o ensino de história: aproximações e distanciamentos

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    Astrogildo Fernandes Silva Júnior

    2011-01-01

    Full Text Available This study has the objective to reflect about the relations between History science and History teaching. In this context, some questions are brought about: Which are the perspectives to put together History science and History teaching? What does the curricular proposal says about History teaching? Searching for answers to these questions, Rusen, Deleuze and Guatarri should be considered. / Este trabalho tem como objetivo refletir sobre a relação entre a ciência da História e o ensino de História. Nesse sentido, algumas questões tornaram-se recorrentes: Quais as perspectivas de aproximação da ciência da História com o ensino de História? O que diz a proposta curricular para o ensino de História? Na procura de respostas às referidas inquietações, podem ser consideradas importantes as obras de Rusen (2007, e Deleuze e Guatarri (1995.

  13. Memória, história e representações: acreditam os replicantes em suas reminiscências?

    Directory of Open Access Journals (Sweden)

    Weber Abrahão Jr

    2016-06-01

    Full Text Available Este artigo resulta de leituras feitas no Mestrado Profissional em História, na UFG, Regional Catalão, para aproveitamento da disciplina Imaginários e Narrativas no Ensino de História. A partir dos conceitos de memória, história e representação, como analisados por diversos autores, o objetivo do artigo é dialogar com livro e filme, icônicos produtos da indústria cultural: Androides Sonham com Ovelhas Elétricas?, é o livro, de Philip Kindred Dick;  Blade Runner, O Caçador de Androides, é sua adaptação cinematográfica. A trama como desenvolvida nas obras, embora distinta em diversos pontos, permite exercício teórico necessário à formação intelectual do professor-pesquisador. Desse modo, o artigo é uma narrativa permeada pela memória afetiva do autor em relação às obras analisadas, constituindo-se em atividade didática a serviço da formação do professor-pesquisador.

  14. Reactivity Initiated Accident Test Series RIA Scoping Test Experiment Operating Specification

    Energy Technology Data Exchange (ETDEWEB)

    N/A

    1978-06-01

    This document describes the experiment operating specifications for the Reactivity Initiated Accident (RIA) Scoping Test to be conducted in the Power Burst Facility (PBF) at the Idaho National Engineering Laboratory. The primary objectives of the RIA research are to determine fuel failure thresholds, modes, and consequences as functions of (a) enthalpy insertion, (b) irradiation history, and (c) fuel design. Coolant conditions of pressure, temperature, and flow rate that are typical of hot-startup conditions in commercial boiling water reactors (BWRs) will be used in the first six RIA tests, termed Series I.

  15. Memória e arquivos digitais: outras maneiras de lembrar e esquecer

    OpenAIRE

    Lisboa, Pablo Fabião

    2011-01-01

    O esquecimento já foi evidenciado como uma necessidade cerebral, sobretudo como uma característica da memória biológica. Todavia, no âmbito das novas tecnologias, o projeto parece ser o de constituir uma memória sócio-tecnológica total. O conceito de memória é encontrado em diversas áreas do conhecimento, mas é no âmbito das tecnologias digitais que ele vai apresentar uma potência intrínseca em registrar dados e informações, os quais dialogam com os objetivos da preservação pa...

  16. Paisagens: práticas, memórias e narrativas

    OpenAIRE

    Pellini, José Roberto

    2015-01-01

    Resumo: antes de ser apenas um espaço neutro e cartesiano, destinado apenas ao olhar distanciado, paisagem é uma apropriação subjetiva que envolve práticas, memórias e narrativas, pois não existe experiência que não seja sensorial, assim como não existe experiência que não seja mediada pela memória e que não esteja situada em um lugar. Palavras-chave: Paisagem. Memória. Sentidos.

  17. Rizobactérias no crescimento e na produtividade da cebola

    OpenAIRE

    Harthmann, Oscar Emilio Ludtke; Mógor,Átila Francisco; Wordell Filho, João Américo; Luz,Wilmar Cório da

    2010-01-01

    A associação de plantas com rizobactérias pode promover o crescimento vegetal, reduzindo custos de produção. As rizobactérias benéficas podem ser uma alternativa para a produção de cebola. Neste trabalho, foi avaliado o efeito da aplicação de rizobactérias no crescimento e na produtividade da cebola da cultivar 'Bola Precoce'. A aplicação foi realizada na Estação Experimental da Epagri, em Ituporanga, Santa Catarina (SC), no ano de 2008. O delineamento experimental foi em blocos ao acaso, com...

  18. Tratamento de sementes com rizobactérias na produção de cebola

    OpenAIRE

    Harthmann, Oscar Emilio Ludtke; Mógor,Átila Francisco; Wordell Filho, João Américo; Luz,Wilmar Cório da; Biasi,Luiz Antonio

    2009-01-01

    A associação de plantas com rizobactérias benéficas pode promover o crescimento vegetal e o biocontrole de doenças, reduzindo custos de produção e diminuindo o impacto dos agrotóxicos no meio ambiente. Com o objetivo de avaliar os efeitos de rizobactérias aplicadas às sementes de cebola 'Bola Precoce' no desenvolvimento de plantas e na produção de bulbos, foi conduzido um estudo na Estação Experimental da Epagri de Ituporanga, Santa Catarina, em 2007. Foram avaliadas as rizobactérias Pseudomo...

  19. A Memória Institucional – Santa Casa POA/RS

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    Margareth de Oliveira Michel

    2016-03-01

    Full Text Available O trabalho analisa os enfoques sobre a memória organizacional e como esse trabalho é articulado: questões estratégicas de resgate e construção de memória; a emergência do “mercado de memórias”, a relação entre a cultura e a memória organizacional, os instrumentos utilizados. São desenvolvidas reflexões teóricas nas áreas estudadas aliadas ao estudo de caso da Santa Casa de Misericórdia de POA.

  20. Psicologia Comunitária e Educação Libertadora

    OpenAIRE

    2009-01-01

    Este artigo tematiza articulações teórico-metodológicas entre a Psicologia Comunitária e a Educação Libertadora, discutindo também suas propostas socioideológicas e ético-políticas. Consiste em um desdobramento de uma pesquisa realizada pelo Núcleo de Psicologia Comunitária (Nucom) da Universidade Federal do Ceará, cuja finalidade era contribuir com o desenvolvimento epistemológico do marco teórico-metodológico da Psicologia Comunitária. Para tanto, procedemos a uma revisão teórica, explorand...

  1. Ensino de História hoje: errâncias, conquistas e perdas

    Directory of Open Access Journals (Sweden)

    Marcos Antônio da Silva

    2010-01-01

    Full Text Available Este artigo analisa tradições de debate sobre Ensino de História no Brasil desde a ditadura de 1964-1984. Ele discute as mudanças, permanências, conquistas e perdas na história da disciplina. Destaca a importância da cultura escolar, a necessária continuidade da escola como instituição e o diálogo com formas não escolares de ensino.

  2. Violència i memòria en Milan Kundera

    OpenAIRE

    Tafalla, Marta

    2007-01-01

    Partint de la filosofia de la memòria de TW Adorno, aquest article defensa la tesi deque la literatura té una major capacitat que la història o la filosofia per explicar Enquin consisteixen els règims totalitaris i per salvaguardar la memòria de les seves víctimes. Amb aquest propòsit, pren com a exemple la literatura de l'escriptor txec Milan Kundera yanaliza algunes de les seves obres, especialment El llibre del riure i l'oblit, La insoportablelevedad l'ésser i el seu assaig L'art de...

  3. A resilência na história de vida de adolescentes institucionalizados

    OpenAIRE

    Batista, Janete Maria da Silva, 1968-

    2012-01-01

    Resumo: Trata-se de pesquisa descritiva de natureza exploratória, alicerçada no referencial metodológico da História de Vida. Foi realizada em uma Instituição de Acolhimento, de outubro a dezembro de 2010, e teve como objetivo compreender o processo de resiliência dos adolescentes institucionalizados. Participaram da pesquisa dez adolescentes entre as faixas etárias treze e dezesseis anos de idade. A coleta da narrativa ocorreu mediante entrevista aberta, e da análise surgiram dois núcleos te...

  4. Postoperative chest physical therapy prevents respiratory complications in patients undergoing esophagectomy Fisioterapia respiratória pós-operatória previne complicações respiratórias em pacientes submetidos à esofagectomia

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    Adriana C. Lunardi

    2011-04-01

    Full Text Available BACKGROUND: Esophagectomy presents the highest rate of postoperative pulmonary complications among all types of upper abdominal surgery. The benefits of chest physical therapy in patients undergoing upper abdominal surgery have been shown by many studies; however, its specific effect in patients receiving esophagectomy has been seldom investigated. OBJECTIVES: This study aimed to compare the frequency of respiratory complications in patients undergoing esophagectomy receiving chest physical therapy compared to no treatment. METHODS: 70 consecutive patients were evaluated retrospectively and allocated to two groups: control group (CG=no physical therapy; n=30 and chest physical therapy group (PTG; n=40. Patients received chest physical therapy which includes lung re-expansion and airway clearance maneuvers. They were not submitted to either noninvasive ventilation or exercises with devices that generate airways positive pressure. All patients were instructed to early mobilization. Information about pre-operative and respiratory complications were collected. Statistic analysis to compare the frequency of respiratory complications was performed by the Z test. The significance level was set to 5%. RESULTS: Patients in the CG and PTG were similar in terms of age, BMI, smoking and drinking status, malignant diseases, surgical and anesthesia duration and types of esophagectomy (p>0.05. Our results show that patients received chest physical therapy after esophagectomy had a lower frequency of respiratory complications (15% vs. 37%, pCONTEXTUALIZAÇÃO: A esofagectomia apresenta a maior taxa de complicações pulmonares pós-operatórias dentre as cirurgias abdominais altas. Os benefícios da fisioterapia respiratória em pacientes submetidos à cirurgia abdominal alta convencional têm sido mostrados na literatura, porém esse efeito na esofagectomia tem sido pouco investigado. OBJETIVOS: Comparar a frequência de complicações respiratórias em dois

  5. A field study of postpartum period of dairy cows by rectal palpation and progesterone profiles in the milk by radioimmunoassay; Contribuicao ao estudo do puerperio de vacas leiteiras atraves de acompanhamento clinico-reprodutivo e determinacao de progesterona por radioimunoensaio

    Energy Technology Data Exchange (ETDEWEB)

    Kozicki, Luis Ernandes; Silva, Marcelo Alves da [Parana Univ., Curitiba, PR (Brazil). Setor de Ciencias Agrarias. Dept. de Medicina Veterinaria]. E-mail: lkozicki@agrarias.ufpr.br; Woellner, Luis Carlos; Shibata, Flavia Kazumi [Parana Univ., Curitiba, PR (Brazil). Centro de Medicina Nuclear

    1998-07-01

    This study was carried out on 18 calved dairy cows, near the town of Curitiba-PR-Brazil. The animals were submitted weekly to a rectal palpation of the genital tract and the uterine involution and ovarian activity were considered. The rectal palpations began from the 7{sup th} day postpartum till the 49{sup th} day. at the same milk samples the first morning milk were taken on the 9{sup th} week. The progesterone (P{sub 4}) level determination of the milk was established by radioimmunoassay. The findings of the research showed: the postpartum oestrus was observed at the 28.5{sup th} day and the corpus luteum was detected on the 34.5{sup th} day. The period from oestrus to the first high progesterone level post partum was 5.5 days. The uterine involution period was carried on the 24.1{sup th} day. The progesterone dominant period was shorter and had duration of 11.5 days on the first oestrus cycle post partum. The progesterone concentration from the first Corpus luteum post partum was low (1.9 ng/ml milk). The anoestrus rate until 49{sup th} day postpartum was 33.3% and the silent heat rate reached 61.1%. (author)

  6. Hormonal evaluation of T{sub 4} and T{sub 3} through radioimmunoassay in younglings of rats with hypothyroidism; Avaliacao hormonal de T{sub 4} e T{sub 3} atraves de radioimunoensaio em filhotes de ratas hipotireoideas

    Energy Technology Data Exchange (ETDEWEB)

    Silveira, M.F.G. [Pernambuco Univ., Recife, PE (Brazil). Dept. de Biofisica e Radiobiologia; Silva, I.M.S.; Pereira, S.S.L.; Souza, G.M.L.; Carvalho, E.F.M.B.; Cavalcante, C.V.G. [Pernambuco Univ., Recife, PE (Brazil). Dept. de Anatomia; Lima Filho, G.L.; Catanho, M.T.J.A. [Faculdade de Formacao de Professores de Nazare da Mata, PE (Brazil). Dept. de Ciencias Naturais

    2000-07-01

    The onset of fetal thyroid function occurs about 17-18 days after conception in the rat. The maternal hypothyroidism which occurs during gestation provokes alteration in the rat after birth. Due to this alteration, we decided to analyze the hormonal modification in the newborn rats. The hypothyroidism was induced in normal dams, which were being treated for 7 days with MMI (in the concentration of 0,03% in drinking water) before mating. Another dam group which was submitted to an induction of hypothyroidism maintained the treatment with MMI for 13 days during gestation. The hormones were assessed by radioimmunoassay technique. It was seen that the rats which were born from hypothyroid dams suffered alterations on its T{sub 4} and T{sub 3} hormone levels concerning to 10, 30 and 60 days after birth. There was also modifications on their weight and size. The growth is affected throughout post-natal life by thyroid hormones, which has a facilitator influence on growth hormone economy, as opposed to the inhibitory effects on TSH economy. The administration of MMI bars the fetal thyroid gland function, causing a decrease of both T{sub 4} and T{sub 3} levels, even after the birth, indicating that the maternal hypothyroidism influences on the post-natal life of the rat. (author)

  7. LEITURA, MEMÓRIA E TEXTO POÉTICO

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    Onici Claro Flores

    2009-02-01

    Full Text Available Este artigo embasou-se em um trabalho pedagógico com o texto poético, propondo-se a analisar e discutir o conceito de memória. A necessidade de revisão conceitual emergiu durante os encontros com um grupo de (14 quatorze professores de Ensino Fundamental e Médio, inscritos nas oficinas de leitura do texto poético - A construção de conhecimentos: competência e fruição na leitura de textos poéticos - desenvolvidas ao longo de dois meses, durante o segundo semestre de 2006. As oficinas integravam o programa A construção de conhecimentos e competência em leitura: formando professores leitores do Mestrado em Letras/UNISC, cujo objetivo era revitalizar as aulas de leitura ministradas pelos professores da rede de ensino pública e particular da região de abrangência da UNISC, através de um processo de qualificação da mediação leitora de textos narrativos, argumentativos e poéticos. O programa previa a introdução de novas abordagens textuais, buscando inter-relacionar atualização teórico-metodológica e leitura na escola.

  8. Optimization of Steering Elements in the RIA Driver Linac

    CERN Document Server

    SchnirmanLessner, Eliane; Ostroumov, Peter

    2005-01-01

    The driver linac of the projected RIA facility is a versatile accelerator, a 1.4-GV, CW superconducting linac designed to simultaneously accelerate several heavy-ion charge states, providing beams from protons at about 1 GeV to uranium at 400 MeV/u at power levels at a minimum of 100 kW and up to 400 kW for most beams. Acceleration of multiple-charge-state uranium beams places stringent requirements on the linac design. A steering algorithm was derived that fulfilled the driver’s real estate requirements, such as placement of steering dipole coils on SC solenoids and of beam position monitors outside cryostats, and beam-dynamics requirements, such as coupling effects induced by the focusing solenoids.* The algorithm has been fully integrated in the tracking code TRACK** and is used to study and optimize the number and position of steering elements that minimize the multiple-beam centroid oscillations and preserve the beam emittance under misalignments of accelerating and transverse focusing elements...

  9. Irradiation Effects on RIA Fragmentation Cu Beam Dump

    CERN Document Server

    Reyes, Susana; Boles, Jason; Stein, Werner; Wirth, Brian

    2005-01-01

    Within the scope of conceptual R&D activities in support of the Rare-Isotope Accelerator (RIA) facility, high priority is given to the development of high-power fragmentation beam dumps. A pre-study was made of a static water-cooled Cu beam dump that can meet requirements for a 400 MeV/u uranium beam. The issue of beam sputtering was addressed and found to be not a significant issue. Preliminary radiation transport simulations show significant damage (dpa) in the vicinity of the Bragg peak of uranium ions. Experimental data show that defects in Cu following neutron or high-energy particle irradiation tend to saturate at doses between 1 and 5 dpa, and this saturation in defect density also results in saturation of mechanical property degradation. However, effects of swift heavy ion irradiation and the production of gaseous and solid transmutant elements still need to be addressed. Initial calculations indicate that He concentrations on the order of 100 appm are produced in the beam dump after several weeks...

  10. Gestão tributária do ICMS

    Directory of Open Access Journals (Sweden)

    Francisco Santana de Souza

    2007-12-01

    Full Text Available Este trabalho analisa as ferramentas essenciais de gestão do Imposto sobre Ope­rações relativas à Circulação de Mercadorias e Serviços (ICMS, sobre Prestações de Serviços de Transporte Interestadual e Intermunicipal e de Comunicações. Para tanto, utiliza-se um procedimento de cálculo desse imposto que permite demons­trar a importância de efetuar corretamente os lançamentos fiscais e contábeis que lhe correspondem. Para esse cálculo foram usados conceitos de matemática fi­nanceira de juros simples e descontos simples comercial e racional, e observado o artigo 33 da Lei do ICMS do Estado de São Paulo, de 1989. Conclui-se que é fundamental uma boa gestão tributária do ICMS, a fim de: a evitar contencioso administrativo tributário que implicaria custos adicionais e desnecessários à orga­nização, e b utilizar corretamente a elisão fiscal para economizar o pagamento excessivo do imposto. Desse modo, o uso adequado desses dois instrumentos re­fletirá positivamente no fluxo de caixa da organização.

  11. História da Arte ou Estória da Arte? Art History or Art Story?

    Directory of Open Access Journals (Sweden)

    Roberto Carvalho de Magalhães

    2008-12-01

    Full Text Available Tomando como ponto de partida os conceitos sobre arte e história expressos por Paul Valéry em seus escritos teóricos, pelo filósofo alemão Konrad Fiedler e pelo historiador e crítico de arte italiano Carlo L. Ragghianti, o autor questiona as formas de se compor a história da arte e os seus desdobramentos pedagógicos. Perplexo diante da confiança nas definições dadas a períodos históricos, o autor contrapõe a verdade e a concretidão das obras existentes - que têm a sua própria linguagem, a sua própria forma - à reconstrução histórica, que não é capaz de fazer o passado reviver plenamente e não deixa de ser a projeção parcial de um olhar num passado que não existe mais e que chega até nós através de fragmentos e de enormes lacunas a serem preenchidas.In this text, the author examines the ways to construct art history and their pedagogical developments. His references are Paul Valéry's concepts about art and history expressed in his theoretical writings, the German philosopher Konrad Fiedler and the Italian historian and art critic Carlo L. Ragghianti. Astonished in the trust others have given to general definitions of historical periods, the author opposes the truth and concreteness of the existing art works of all times - that have their own languages, their own structures - to the historical reconstruction, which is not able to make the past revive fully, and is always a partial glaze to a past that doesn't exist anymore, which arrives to us fragmented and with enormous gaps to be filled.

  12. História para educadores: o objeto de estudo da área de História

    Directory of Open Access Journals (Sweden)

    Adreana Dulcina Platt

    2014-08-01

    Full Text Available Dispostas em particulares disciplinas, as áreas expressam específicos olhares voltados à "produção da humanidade"em cada sujeito (Saviani, 1991. Tal realidade tem sua "gênese"e desenvolvimento na divisão do trabalho "manual"e "intelectual". Ao desenharmos a relevância do que será apreendido em cada área do conhecimento para a formação de amplas e seguidas gerações, partimos ao anúncio do objeto de estudo dos saberes norteadores das práticas produtivas, culturais e técnico-científicas marcados num determinado tempo e lugar históricos, e expostos institucionalmente numa carta curricular. Pautados nessa perspectiva, elegemos a área de História para investigarmos seu objeto de estudo. Selecionamos um universo de 103 docentes da disciplina de História da rede de ensino em Londrina/PR (Brasil para responderem questões sobre o específico desta área. Sistematizados os dados, compilamos os resultados apoiados no método de Análise de Conteúdo de Bardin (2004. Seguindo a proposta, organizamos os dados conforme a frequência das respostas, elegendo as palavras com conteúdo categorial significativo para a área e conforme sua maior incidência. Pelos resultados, aduzimos introdutoriamente uma convergência nas respostas pela identificação de um objeto significativamente descrito na regularidade discursiva, ou seja, o foco a ser perseguido por certa geração de professores na defesa epistêmica da área de estudo pela temática: o homem no tempo. Da mesma forma, o estudo identifica certas tendências, socialmente determinantes -veiculadas em academias e programas de capacitação-, e relevantes, enquanto fonte de análise, para o reconhecimento do eixo praxiológico de área do conhecimento condutor de uma dada racionalidade curricular.

  13. A história oral como prática no ensino de história: primeiras reflexões acerca de um projeto de pesquisa

    Directory of Open Access Journals (Sweden)

    Felipe Nobrega Ferreira

    2008-04-01

    Full Text Available As possibilidades de se trabalhar com a perspectiva da história oral em um projeto educacional são cada vez mais emergentes no cenário escolar, pois tal metodologia permite com que o aluno vivencie a história em nível prático. Por isso, o presente artigo tem o objetivo de trazer à baila as primeiras considerações de um projeto dentro do ensino escolar de História que vem sendo efetuado desde agosto de 2007 e que opera a partir das prerrogativas da oralidade. Assim, fazem-se presentes neste artigo as atividades realizadas pelo projeto desde então, bem como as reflexões e algumas conclusões preliminares sobre o alcance de tal proposta.

  14. Mecanismo de centralização: da insuficiência placentária à adaptação circulatória fetal

    OpenAIRE

    Villas-Bôas, Juliana Marques Simões [UNESP; Maestá, Izildinha; Consonni,Marcos

    2008-01-01

    A aplicação e o desenvolvimento da doplervelocimetria obstétrica apresentam base para conhecimento da insuficiência placentária e comprovam o comportamento dinâmico da circulação fetal em regime de hipóxia. Na prática clínica, tornou-se quase rotineira a necessidade de se avaliar a hemodinâmica em três territórios vasculares envolvidos na gestação: artérias uterinas, umbilical e cerebral média. Em linhas gerais, a artéria cerebral expressa o balanço entre a oferta de oxigênio nas uterinas e a...

  15. No limiar da História e da Memória: um estudo de \\'Mes Mémoires\\', de Alexandre Dumas

    OpenAIRE

    Maria Lúcia Dias Mendes

    2007-01-01

    A tese faz uma leitura da obra Mes mémoires de Alexandre Dumas a partir da visão de Georges Gusdorf sobre as escrituras do eu. A pesquisa parte da hipótese de que mesmo sendo uma obra memorialística mescla interesses e técnicas utilizadas por Dumas em outras obras. No início, as idéias de Philippe Lejeune e de Georges Gusdorf são apresentadas, e o conceito de memória esboçado. Devido à profunda ligação entre memória e História, analisou-se as mudanças ocorridas no início do século XIX na hist...

  16. Um olhar complexo sobre o passado: história, historiografia e ensino de história no pensamento de Edgar Morin.

    OpenAIRE

    Rodrigues, André Wagner

    2011-01-01

    Esta dissertação apresenta os resultados de pesquisa bibliográfica que teve como objeto, a partir do diagnóstico de limitações teóricas e metodológicas presentes nos estudos e no ensino de História, investigar possíveis contribuições da Teoria da Complexidade de Edgar Morin para interpretações alternativas do passado histórico e para o ensino da disciplina história na educação escolar. Apresenta inicialmente uma trajetória histórica da evolução do pensamento historiográfico clássico, desde o ...

  17. Estuary-ria exchange of cadmium, lead and zinc in the coastal system of the Ria of Vigo (NW Iberian Peninsula

    Directory of Open Access Journals (Sweden)

    Ricardo Prego

    2010-11-01

    Full Text Available Little research has been done on the land-sea exchange of trace elements, which is particularly applicable to ria coasts. In particular, trace metal enrichment in the inner part of the Ria of Vigo (the San Simon Inlet has been observed from sediment studies but there is no information about Cd, Pb and Zn fluxes through the Rande Strait, which is the natural boundary of the estuary-ria water exchange. In order to assess metal exchanges in a ria-type system, six sampling cruises on board the R/V Mytilus (IIM-CSIC were carried out. Water column profiles of salinity, temperature and tidal currents were obtained every 30 min. The water column for dissolved and particulate metals was sampled every two hours over a complete tidal cycle. Dissolved metal concentrations were 0.01–0.18 nM for Cd, 0.5-1.9 nM for Pb and 4-44 nM for Zn. Compared with Zn (16±12% and especially with Cd (5.4±5.0%, particulate metal represented a significant fraction of the total concentration for Pb (41±21%. Net fluxes of dissolved Cd and Zn are higher than in the particulate phase, whereas for Pb an inverse situation was observed. The net metal exchange in the Vigo estuary-ria environment was not seasonally controlled. Dissolved Cd and Pb were driven by tidal ranges and particulate Pb by the Oitavén River flow. On the other hand, Zn did not show a defined trend. The budgets obtained for the Ria of Vigo, with the exception of Pb, were one or two orders of magnitude lower than those measured in other large European estuaries.

  18. HABILIDADES COGNITIVAS EM IDOSAS INSTITUCIONALIZADAS: ESTUDO COMPARATIVO DO DESEMPENHO DE USUÁRIAS E NÃO USUÁRIAS DE BENZODIAZEPINICOS

    Directory of Open Access Journals (Sweden)

    Mônica Giaretton Bicca

    2010-01-01

    Full Text Available Atualmente, constata-se um crescimento significativo da população de idosos. O presente artigo objetivou comparar habilidades cognitivas, como atenção e fluência verbal, em idosas usuárias e não usuárias de benzodiazepínicos. Este estudo transversal contou com uma amostra de 123 idosas institucionalizadas da cidade de Porto Alegre, RS. Os resultados apontam que não houve associação significativa entre uso de benzodiazepínicos e as habilidades cognitivas das idosas avaliadas. Palavras-chave: habilidades cognitivas; idosas; benzodiazepínicos.

  19. Entre a filosofia e a sociologia: matrizes teóricas das discussões atuais sobre história e memória

    Directory of Open Access Journals (Sweden)

    Schmidt, Benito Bisso

    2006-01-01

    Full Text Available O objetivo deste texto é situar as matrizes teóricas dos trabalhos de alguns autores que servem como referência para boa parte dos estudos contemporâneos sobre as relações entre história e memória: Bergson, Halbwachs, Pollak, Nora e Ricoeur. Seus estudos dialogam, de maneira direta ou indireta, com duas matrizes: a sociológica-objetivista e a fenomenológica-intuicionista. Elas se estabeleceram no âmbito da disputa entre a sociologia e a filosofia pelo direcionamento das ciências humanas nascentes no século XIX

  20. Anastomose mamária-coronária: análise de 2923 casos Mammary-coronary artery anastomosis: analysis of 2923 cases

    Directory of Open Access Journals (Sweden)

    Jarbas J Dinkhuysen

    1987-04-01

    Full Text Available Foi a partir de 1972 que, no Instituto Dante Pazzanese de Cardiologia, se iniciou o emprego da anastomose mamária-coronária associada, ou não, a pontes de safena, ou a outros procedimentos. Nessa época, apenas a artéria mamária interna esquerda (AMIE foi usada (57 casos para a revascularização da descendente anterior (DA ou diagonal. No período 1973/1974 (386 casos, já ocorreram as primeiras anastomoses seqüenciais AMIE-DA/DA, assim como a artéria mamária interna direita (AMID passou a ser empregada para ramos diagonais. Com a finalidade de verificar a evolução tardia desta técnica, sua aplicação foi praticamente interrompida, entre 1975/1982, restringindo-se a apenas 43 casos, nesse período. Entretanto, a partir de 1982 até junho de 1986, ocorreu significativo incremento na sua aplicação (2374 casos, sendo que, desde então, a quase totalidade (93% das cirurgias de revascularização do miocárdio tem uma ou mais coronárias tratadas com anastomose mamária-coronária. A combinação mais freqüente é AMIE/DA nos casos de mamária isolada e AMIE/DA e AMID/Mg Cx nos casos de dupla mamária. Contudo, observaram-se, na casuística, as mais variadas combinações (71 e associações, abrangendo, praticamente, todas as artérias coronárias. Analisando-se o pós-operatório imediato de um grupo isolado de 177 pacientes operados em 1984, observou-se que esta técnica não influiu na ocorrência de deiscência parcial, ou total, de esterno, em reoperação por sangramento; todavia, ocorreram, em 25% dos casos, elevação transitória da cúpula frénica, atelectasia em 17% e derrame pleural residual em 27,6%, geralmente concomitantes. Em relação à evolução tardia, num grupo de 754 pacientes com mamária e safena e com evolução até 9 anos, em 924 (71,8% dos enxertos reestudados, constataram-se índices de permeabilidade de 91,5% nas mamárias versus 70,6% (826 das pontes de safena. Em outro grupo (102 pacientes, com evolu

  1. Caraterização dos utentes das consultas de periodontologia na Clínica Dentária Universitária Egas Moniz

    OpenAIRE

    Afonso, André Filipe Rodrigues

    2016-01-01

    Dissertação para obtenção do grau de Mestre no Instituto Superior de Ciências da Saúde Egas Moniz Objetivo: caraterizar os utentes das consultas de periodontologia da clínica dentária universitária Egas Moniz, procurando identificar os fatores de risco mais associados à doença periodontal (DP) desta população. Material e método: estudo observacional, analítico e transversal, efetuado numa amostra de conveniência de 150 utentes das consultas de periodontologia, aos quais se aplicou previ...

  2. Caraterização dos utentes das consultas de periodontologia na Clínica Dentária Universitária Egas Moniz

    OpenAIRE

    Afonso, André Filipe Rodrigues

    2016-01-01

    Dissertação para obtenção do grau de Mestre no Instituto Superior de Ciências da Saúde Egas Moniz Objetivo: caraterizar os utentes das consultas de periodontologia da clínica dentária universitária Egas Moniz, procurando identificar os fatores de risco mais associados à doença periodontal (DP) desta população. Material e método: estudo observacional, analítico e transversal, efetuado numa amostra de conveniência de 150 utentes das consultas de periodontologia, aos quais se aplicou previ...

  3. O espaço político aberto pela leitura literária

    Directory of Open Access Journals (Sweden)

    Leonardo Pinto de Almeida

    2013-01-01

    Full Text Available O presente artigo se propõe questionar sobre o espaço político aberto pela leitura literária. Especificando a literatura como uma tentativa de compreensão de problemas relacionados à existência, sublinhamos como a leitura literária coloca em questão nossas convicções e verdades defendidas cotidianamente. Para tanto, compreendemos as relações tecidas entre a linguagem, o poder e a resistência no seio da experiência literária. Concluímos que a leitura literária é atravessada por formas de resistência às palavras de ordem que circulam de forma hegemônica em nossa sociedade, disponibilizando assim uma transformação em nossa rede afetiva e cognitiva.

  4. EXPANSÃO DA ECONOMIA SOLIDÁRIA NO SUL DO BRASIL

    OpenAIRE

    Ribeiro, Bolívar Alencar

    2013-01-01

    Este trabalho foi baseado em uma análise detalhada das informações disponibilizadas pelo SIES/MTE, maior fonte de dados sobre a economia solidária no Brasil. O objeto de estudo foi a expansão da economia solidária na região sul do Brasil. Dessa forma teve por objetivo geral compreender e analisar a expansão da economia solidária nessa região. Foi constatada uma expansão considerável de empreendimentos sob os princípios da Economia Solidária na região, ocorrida em meio à crise do emprego forma...

  5. Milliseid muutusi näete 21. sajandi koolis? / Eduardo Chaves, Ria Sluiter, Rafael Enriques

    Index Scriptorium Estoniae

    Chaves, Eduardo

    2007-01-01

    Küsimusele vastavad: Microsofti programmi "Partners in Learning" nõunik Brasiiliast Eduardo Chaves, Hollandi keskhariduse spetsailist Ria Sluiter ja Mexico Interaktiivse Lastemuuseumi juhataja Rafael Enriques 21. sajandi koolist oma maa hariduskorralduste põhjal

  6. FRAGMENTOS DO PRETÉRITO: REFLEXÕES ACERCA DA MEMÓRIA INDIVIDUAL E COLETIVA

    OpenAIRE

    Chagas Oliveira, Priscila

    2016-01-01

    Este artigo realiza revisão de literatura sobre a temática da memória e seus complexos processos de seleção, armazenamento e evocação, que influem, a nível individual e coletivo, na construção das identidades. Apresenta pesquisas sobre o cérebro explorando a memória como faculdade e fenômeno. Problematiza o conceito de memória coletiva, sugerindo reflexão à noção de compartilhamento e salienta o papel do esquecimento nos processos mnemônicos. Considera o estatuto da memória na era das redes e...

  7. Hanna Krall e os caminhos tortos da memória e da identidade

    Directory of Open Access Journals (Sweden)

    Piotr Kilanowski

    2013-08-01

    Full Text Available Neste artigo apresentamos a escritora polonesa de origem judaica Hanna Krall por meio da análise literária do seu romance Sublokatorka (A sublocatária.  O romance, com elementos autobiográficos, apresenta a personalidade cindida de uma menina sobrevivente do Holocausto e discute o tema da identidade polonesa, judaica e judaico-polonesa. Através da observação da memória e do processo de criação da memória, a autora mostra a construção da identidade falsa e a repressão da verdadeira, o comportamento comum entre alguns dos sobreviventes.

  8. Aneurisma gigante da artéria cerebral posterior: (segmento P3) Relato de caso

    OpenAIRE

    1995-01-01

    Apresentamos caso de aneurisma gigante do segmento distal (P3) da artéria cerebral posterior. O aneurisma foi tratado microcirurgicamente pela via combinada proposta por Sano com clipagem do ramo terminal da artéria cerebral posterior próximo ao aneurisma. A paciente teve evolução pós operatória favorável, com lesão parcial e transitória do III nervo craniano. Revisão da literatura e discussão a respeito da anatomia e da abordagem cirúrgica são feitas. A raridade desta patologia justifica o r...

  9. Milliseid muutusi näete 21. sajandi koolis? / Eduardo Chaves, Ria Sluiter, Rafael Enriques

    Index Scriptorium Estoniae

    Chaves, Eduardo

    2007-01-01

    Küsimusele vastavad: Microsofti programmi "Partners in Learning" nõunik Brasiiliast Eduardo Chaves, Hollandi keskhariduse spetsailist Ria Sluiter ja Mexico Interaktiivse Lastemuuseumi juhataja Rafael Enriques 21. sajandi koolist oma maa hariduskorralduste põhjal

  10. Anatomia coronária por tomografia computorizada multi-corte e principais variantes

    OpenAIRE

    Gomes, André Santos Teixeira

    2010-01-01

    A avaliação das artérias coronárias assume uma elevada importância na prática clínica actual dado que as doenças cardiovasculares são a primeira causa de morte nos países desenvolvidos. A técnica de eleição para o estudo coronário é a Angiografia Coronária por cateterismo, porém, esta acarreta alguns riscos. A Coronáriografia por Tomografia Computorizada (CTC) multi-corte tem vindo a posicionar-se como o melhor exame não invasivo para avaliação coronária, graças à rápida evoluç...

  11. A economia popular solidária em Santa Maria – RS e suas interfaces

    Directory of Open Access Journals (Sweden)

    Juliana Franchi da Silva

    2015-12-01

    Full Text Available O presente artigo tem por objetivo apresentar e analisar o crescimento da economia solidária no contexto da sociedade capitalista e compreender como se processou e tem se mantido o projeto da feira popular solidária da cidade de Santa Maria – RS que já completou 20 anos de existência. A Economia Solidária constitui-se como uma forma alternativa de gestão para que os produtores urbanos e rurais se organizem para também competirem no mercado convencional. E assim, diferencia-se dos princípios que regem o sistema capitalista, pois está centrada na perspectiva de relações sociais solidárias e não em competição.

  12. Atividade antiinflamatória de quefir, um probiótico da medicina popular

    Directory of Open Access Journals (Sweden)

    R.O. Diniz

    2003-01-01

    Full Text Available O quefir é uma bebida fermentada originária do Cáucaso e formada por bactérias ácido-lácticas e leveduras, sendo utilizada por suas propriedades organolépticas e terapêuticas. O presente estudo avaliou a atividade antiinflamatória deste probiótico sobre modelo de indução de tecido granulomatoso e contorções abdominais induzidas por ácido acético, in vivo. Os resultados obtidos mostraram que o quefir inibiu a formação do tecido granulomatoso, bem como o número de contorções abdominais causadas por ácido acético, quando comparado ao grupo controle. Desta forma, pode-se sugerir uma possível ação antiinflamatória.

  13. Thorium accumulation in the sedimentary environment of the Vigo Ria (NW Iberian Peninsula).

    Science.gov (United States)

    Marmolejo-Rodríguez, Ana J; Caetano, Miguel; Prego, Ricardo; Vale, Carlos

    2008-10-01

    Thorium levels and their association with Fe, Sc, Ca, and Particulate Organic Carbon (POC) were researched in sediments of the Vigo Ria in order to assess the influence of radioactive gneisses from the neighbouring continental Galiñeiro Complex. Sediment of 50 surface samples and 1 core was analysed by ICP-MS and the results were validated with certificate reference material from marine sediment. It was found that Th accumulates in the middle Ria zone (9-15 mg kg(-1)) as a result of the terrestrial source. The thorium concentration in the Ria (average: 9.4+/-5.1 mg kg(-1)) is not anthropogenically impacted in accordance with its own regional background level (11.4+/-3.5 mg kg(-1)), which is two times higher than the content in earth's crust. Throughout the relationship Th/Fe, Th may be associated with the iron cycle in the Ria, probably through iron oxy-hydroxide scavenging.

  14. Reologia dos lodos e de outros sedimentos recentes da Ria de Aveiro

    OpenAIRE

    Bonito, Fernando Alcino Borges

    2008-01-01

    Nos últimos anos têm sido construídas e estão projectadas para a construção várias obras de engenharia civil na região de Aveiro, destacando-se algumas infra-estruturas rodoviárias, ferroviárias e portuárias cujo funcionamento em pleno reveste-se de particular importância dados os seus impactos globalmente positivos na estruturação do tecido socioeconómico da região e do país. Por outro lado, aspectos como a localização geográfica litoral e o enquadramento geológico da cidade de Avei...

  15. Democracia e economia solidária: limitações e potencialidades

    National Research Council Canada - National Science Library

    Locks, Pompilio; Gugliano, Alfredo Alejandro

    2013-01-01

    ... no âmbito da economia solidária. Enquanto para um segmento da literatura acadêmica as associações produtivas contrariam a empresa capitalista e aprofundam a democracia, outro lado rejeita essas possibilidades...

  16. Coastal upwelling and downwelling forcing of circulation in a semi-enclosed bay: Ria de Vigo

    Science.gov (United States)

    Barton, E. D.; Largier, J. L.; Torres, R.; Sheridan, M.; Trasviña, A.; Souza, A.; Pazos, Y.; Valle-Levinson, A.

    2015-05-01

    Semi-enclosed bays in upwelling regions are exposed to forcing related to winds, currents and buoyancy over the shelf. The influence of this external forcing is moderated by factors such as connectivity to the open ocean, shelter by surrounding topography, dimensions of the bay, and freshwater outflows. Such bays, preferred locations for ports, mariculture, marine industry, recreational activities and coastal settlement, present a range of characteristics, understanding of which is necessary to their rational management. Observations in such a semi-enclosed bay, the Ria de Vigo in Spain, are used to characterize the influence of upwelling and downwelling pulses on its circulation. In this location, near the northern limit of the Iberian upwelling system, upwelling events dominate during a short summer season and downwelling events the rest of the year. The ria response to the external forcing is central to nutrient supply and resultant plankton productivity that supports its high level of cultured mussel production. Intensive field studies in September 2006 and June 2007 captured a downwelling event and an upwelling event, respectively. Data from eight current profiler moorings and boat-based MiniBat/ADCP surveys provided an unprecedented quasi-synoptic view of the distribution of water masses and circulation patterns in any ria. In the outer ria, circulation was dominated by the introduction of wind-driven alongshore flow from the external continental shelf through the ria entrances and its interaction with the topography. In the middle ria, circulation was primarily related to the upwelling/downwelling cycle, with a cool, salty and dense lower layer penetrating to the inner ria during upwelling over the shelf. A warmer, lower salinity and less dense surface layer of coastal waters flowed inward during downwelling. Without external forcing, the inner ria responded primarily to tides and buoyancy changes related to land runoff. Under both upwelling and downwelling

  17. Comparison of Measurements of Autoantibodies to Glutamic Acid Decarboxylase and Islet Antigen-2 in Whole Blood Eluates from Dried Blood Spots Using the RSR-Enzyme Linked Immunosorbent Assay Kits and In-House Radioimmunoassays

    Directory of Open Access Journals (Sweden)

    Anders Persson

    2010-01-01

    Full Text Available To evaluate the performance of dried blood spots (DBSs with subsequent analyses of glutamic acid decarboxylase (GADA and islet antigen-2 (IA-2A with the RSR-ELISAs, we selected 80 children newly diagnosed with type 1 diabetes and 120 healthy women. DBSs from patients and controls were used for RSR-ELISAs while patients samples were analysed also with in-house RIAs. The RSR-ELISA-GADA performed well with a specificity of 100%, albeit sensitivity (46% was lower compared to in RIA (56%; P=.008. No prozone effect was observed after dilution of discrepant samples. RSR-ELISA-IA-2A achieved specificity of 69% and sensitivity was lower (59% compared with RIA (66%; P<.001. Negative or low positive patients and control samples in the RSR-ELISA-IA-2A increased after dilution. Eluates from DBS can readily be used to analyse GADA with the RSR-ELISA, even if low levels of autoantibodies were not detected. Some factor could disturb RSR-ELISA-IA-2A analyses.

  18. Evaluation of safety criteria on LOCA and RIA for high burnup nuclear fuel

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Sun Ki; Bang, Je Geon; Kim, Dae Ho; Yang, Yong Sik; Song, Keun Woo

    2007-12-15

    Comprehensive researches in many countries and some international research programs to investigate the applicability to high burnup nuclear fuels have been performing as the existing safety criteria of DBA such as LOCA and RIA was established several decades ago. In this report, main research programs for the safety criteria of DBA such as LOCA and RIA are introduced, and also the current status on the modification of the safety criteria are also introduced.

  19. Dificuldades encontradas pelos indiv??duos com Fenilceton??ria em aderir a dietoterapia

    OpenAIRE

    Leite, Aluna: Bruna Vilela Silva

    2015-01-01

    A fenilceton??ria ?? um erro inato do metabolismo caracterizado por defici??ncia ou aus??ncia de atividade da enzima fenilalanina hidroxilase, o que impede a convers??o de fenilalanina em tirosina, podendo trazer efeitos t??xicos para o sistema nervoso central, trazendo sequelas irrevers??veis. A presente pesquisa teve como objetivo avaliar as dificuldades encontradas por indiv??duos com fenilceton??ria em aderir ?? dietoterapia, tomando como base, moradores do Distrito Federal. Trata -se de ...

  20. Humor e ensino: J. Carlos e a caricatura no Ensino de História

    Directory of Open Access Journals (Sweden)

    Daniela Cardoso da Silva

    2015-05-01

    Full Text Available O propósito deste artigo é dar um breve histórico da caricatura, analisando-a como instrumento para o Ensino de História, apontando como acontece este diálogo entre a arte caricata de J.Carlos no período chamado Estado Novo, sua representação de mundo e o ensino de História.