Cheng, C.H.; Wooten, G.F.
1981-03-01
The relative brain tissue concentrations of dopamine (DA) and its deaminated metabolite, dihydroxyphenylacetic acid (DOPAC), appears to be a reliable index of the functional activity of dopaminergic neurons. In order to apply this approach to the assessment of dopaminergic neuronal activity in small regions of brain, we have developed a sensitive radioenzymatic assay for simultaneous measurement of DA and DOPAC. The sensitivity of the assay for DA is approximately 10 pg and for DOPAC 100 pg. In addition, the assay is highly specific, simple, and relatively inexpensive. The concurrent estimation of tissue DA and DOPAC concentrations seems to be a reliable means of evaluating the rate of DA turnover or release in behavioral, electrical stimulation, and certain drug paradigms. However, the release or turnover of DA as induced by D-amphetamine (and perhaps other indirectly-acting dopaminemimetic drugs) cannot be meaningfully assessed by measurement of DA and DOPAC alone.
Hussain, M.N.; Benedict, C.R.
1987-06-01
This paper describes several modifications of the original radioenzymatic assay for serotonin which increase the sensitivity of the assay 20-fold as well as enhance its reliability. Using this method serotonin concentrations can be directly measured in biological examples without precleaning the sample. When compared to currently available methods this assay is specific and sensitive to approximately 1 pg of serotonin and can be used to measure serotonin levels in individual brain nuclei or microliter quantities of biological fluids. This assay can be easily adapted for the direct measurement of N-acetylserotonin. A large number of samples can be assayed in a single working day.
Diagnosis of bovine brucellosis using a homogeneous fluorescence polarization assay.
Nielsen, K; Gall, D; Lin, M; Massangill, C; Samartino, L; Perez, B; Coats, M; Hennager, S; Dajer, A; Nicoletti, P; Thomas, F
1998-12-11
To evaluate the fluorescence polarization assay (FPA) for the serological diagnosis of bovine brucellosis, 118 sera from cattle which were culture positive for Brucella abortus, 1751 sera from cattle from premises containing cattle infected with B. abortus, 1222 sera from cattle vaccinated with B. abortus strain 19 and 1199 sera from cattle with no evidence of brucellosis were tested in Argentina, Chile, Mexico and in the American states of Iowa, Missouri and Texas. Initial determination of serological positivity and negativity was based upon reactivity in currently used serological tests, consisting of a rapid screening test, the rose-bengal or the buffered plate antigen tests, followed by a second serological test, the complement fixation test. Sensitivity of the FPA (sera from culture positive animals) ranged from 87.5% to 100%. Serological positivity of cattle from infected premises ranged from 65.5% to 99.0% while the % negative cattle in herds without evidence of brucellosis was between 94.9 and 100%. Of B. abortus strain 19 vaccinated cattle which were positive in at least one in-use serological tests, 88.2% were negative in the FPA. In contrast, previous Canadian studies, sensitivity values were 99.0% and 100% and the specificity in both cases was 100%. This discrepancy was probably due to the use of less well characterized sera in the current study.
Development of homogeneous luminescence assays for histone demethylase catalysis and binding.
Kawamura, Akane; Tumber, Anthony; Rose, Nathan R; King, Oliver N F; Daniel, Michelle; Oppermann, Udo; Heightman, Tom D; Schofield, Christopher
2010-09-01
Covalent modifications to histones play important roles in chromatin dynamics and the regulation of gene expression. The JumonjiC (JmjC)-containing histone demethylases (HDMs) catalyze the demethylation of methylated lysine residues on histone tails. Here we report the development of homogeneous luminescence-based assay methods for measuring the catalytic activity and the binding affinities of peptides to HDMs. The assays use amplified luminescent proximity homogeneous assay (ALPHA) technology, are sensitive and robust, and can be used for small molecule inhibitor screening of HDMs. We have profiled known inhibitors of JMJD2E and demonstrate a correlation between the inhibitor potencies determined by the ALPHA and other types of assays. Although this study focuses on the JMJD2E isoform, the catalytic turnover and binding assays described here can be used in studies on other HDMs. The assays should be useful for the development of small molecule inhibitors selective for HDM isoforms.
C.M. Cobbaert (Christa); M. Nauck; L. Zwang (Louwerens); F. Ceriotti; A. Modenese; P. Cremer; W. Herrmann; G. Hoss; J. Jarausch; R. Turk; W. Marz
1998-01-01
textabstractA homogeneous HDL-c assay (HDL-H), which uses polyethylene glycol-modified enzymes and sulfated alpha-cyclodextrin, was assessed for precision, accuracy, and cholesterol and triglyceride interference. In addition, its analytical performance was compared with
C.M. Cobbaert (Christa); M. Nauck; L. Zwang (Louwerens); F. Ceriotti; A. Modenese; P. Cremer; W. Herrmann; G. Hoss; J. Jarausch; R. Turk; W. Marz
1998-01-01
textabstractA homogeneous HDL-c assay (HDL-H), which uses polyethylene glycol-modified enzymes and sulfated alpha-cyclodextrin, was assessed for precision, accuracy, and cholesterol and triglyceride interference. In addition, its analytical performance was compared with
Peptide Binding to HLA Class I Molecules: Homogenous, High-Throughput Screening, and Affinity Assays
Harndahl, Mikkel; Justesen, Sune Frederik Lamdahl; Lamberth, Kasper;
2009-01-01
present a homogenous, proximity-based assay for detection of peptide binding to HLA class I molecules. It uses a conformation-dependent anti-HLA class I antibody, W6/32, as one tag and a biotinylated recombinant HLA class I molecule as the other tag, and a proximity-based signal is generated through...
Optimization of a homogeneous assay for kinase inhibitors in plant extracts.
Dufau, Isabelle; Lazzari, Anne; Samson, Arnaud; Pouny, Isabelle; Ausseil, Frederic
2008-10-01
To identify natural and original kinase inhibitors from plant extracts, we have developed and compared a heterogeneous enzyme-linked immunosorbent assay (ELISA) and a homogeneous time-resolved fluorescence (HTRF, Cisbio International, Bagnols/Cèze, France) assay. Kinase affinity for the ATP substrate was determined in both assays, and the same [ATP]/ATP Km ratio was used in each case to enable the identification of ATP competitive and noncompetitive inhibitors. Assays were then used to screen the same collection of chemical compounds and plant extracts. The intra-assay correlation analysis of each technology showed a very good screening precision in HTRF and an acceptable one in ELISA. When the two methods were compared, a poor correlation was obtained with a higher hit rate in the ELISA. We then performed a detailed study of the ELISA hits and showed that they also presented a strong antioxidant activity, associated with high adsorption into microplate wells, which interfered with the horseradish peroxidase-based detection system. These hits were then flagged as false-positives. We also showed that many plant extracts presented this kind of activity and that this interference could explain the lack of correlation between the assays. These findings suggest that assay design should be carefully adapted to the substances to be screened and that interferences should be extensively considered before any assay development process and comparison studies. In spite of a few interferences, our results showed that a homogeneous-phase assay like the HTRF assay could be more efficiently used for plant extract screening than a heterogeneous-phase assay like ELISA.
A Homogeneous HLA-B*27 Genotyping Assay Using Dried Reagent Mixtures
Minna Kiviniemi
2009-01-01
Full Text Available The presence of HLA-B*27 allele with patients suspected with ankylosing spondylitis can be used in the diagnostic process. We have developed an assay for typing for the HLA-B*27 in whole blood dried on sample collection cards using pre-dried reagent wells and homogeneous time-resolved fluorescence based PCR approach. Essentially only the sample needs to be added to the dry ready-to-use reaction well in order to start the homogenous amplification assay. The method was validated with 229 samples also typed with an existing DELFIA-based method and results of both assays were 100% concordant. The dried reagents were shown to be stable at least up to eight weeks at room temperature without any decline in their performance.
A universal homogeneous assay for high-throughput determination of binding kinetics.
Schiele, Felix; Ayaz, Pelin; Fernández-Montalván, Amaury
2015-01-01
There is an increasing demand for assay technologies that enable accurate, cost-effective, and high-throughput measurements of drug-target association and dissociation rates. Here we introduce a universal homogeneous kinetic probe competition assay (kPCA) that meets these requirements. The time-resolved fluorescence energy transfer (TR-FRET) procedure combines the versatility of radioligand binding assays with the advantages of homogeneous nonradioactive techniques while approaching the time resolution of surface plasmon resonance (SPR) and related biosensors. We show application of kPCA for three important target classes: enzymes, protein-protein interactions, and G protein-coupled receptors (GPCRs). This method is capable of supporting early stages of drug discovery with large amounts of kinetic information.
Discovery of novel PDE10 inhibitors by a robust homogeneous screening assay
Qun-yi LI; Ming-kai XU; Gang LIU; Claus Tornby CHRISTOFFERSEN; Ming-wei WANG
2013-01-01
Aim:To develop a homogeneous assay for high-throughput screening (HTS) of inhibitors of phosphodiesterase 10 (PDE10).Methods:Purified human PDE10 enzyme derived from E coli,[3H]-cAMP and yttrium silicate microbeads were used to develop an HTS assay based on the scintillation proximity assay (SPA) technology.This method was applied to a large-scale screening campaign against a diverse compound library and subsequent confirmation studies.Preliminary structure-activity relationship (SAR) studies were initiated through limited structural modifications of the hits.Results:The IC50 value of the control compound (papaverine) assessed with the SPA approach was comparable and consistent with that reported in the literature.Signal to background (S/B) ratio and Z' factor of the assay system were evaluated to be 5.24 and 0.71,respectively.In an HTS campaign of 71 360 synthetic and natural compounds,67 hits displayed reproducible PDE10 inhibition,of which,8 were chosen as the scaffold for structural modifications and subsequent SAR analysis.Conclusion:The homogeneous PDE10 SPA assay is an efficient and robust tool to screen potential PDE10 inhibitors.Preliminary SAR studies suggest that potent PDE10 inhibitors could be identified and developed through this strategy.
Vergauwe, Nicolas; Witters, Daan; Ceyssens, Frederik; Vermeir, Steven; Verbruggen, Bert; Puers, Robert; Lammertyn, Jeroen
2011-05-01
Electrowetting-on-dielectric (EWOD) lab-on-a-chip systems have already proven their potential within a broad range of bio-assays. Nevertheless, research on the analytical performance of those systems is limited, yet crucial for a further breakthrough in the diagnostic field. Therefore, this paper presents the intrinsic possibilities of an EWOD lab-on-a-chip as a versatile platform for homogeneous and heterogeneous bio-assays with high analytical performance. Both droplet dispensing and splitting cause variations in droplet size, thereby directly influencing the assay's performance. The extent to which they influence the performance is assessed by a theoretical sensitivity analysis, which allows the definition of a basic framework for the reduction of droplet size variability. Taking advantage of the optimized droplet manipulations, both homogeneous and heterogeneous bio-assays are implemented in the EWOD lab-on-a-chip to demonstrate the analytical capabilities and versatility of the device. A fully on-chip enzymatic assay is realized with high analytical performance. It demonstrates the promising capabilities of an EWOD lab-on-a-chip in food-related and medical applications, such as nutritional and blood analyses. Further, a magnetic bio-assay for IgE detection using superparamagnetic nanoparticles is presented whereby the nanoparticles are used as solid carriers during the bio-assay. Crucial elements are the precise manipulation of the superparamagnetic nanoparticles with respect to dispensing and separation. Although the principle of using nano-carriers is demonstrated for protein detection, it can be easily extended to a broader range of bio-related applications like DNA sensing. In heterogeneous bio-assays the chip surface is actively involved during the execution of the bio-assay. Through immobilization of specific biological compounds like DNA, proteins and cells a reactive chip surface is realized, which enhances the bio-assay performance. To demonstrate
A robust homogeneous binding assay for α4β2 nicotinic acetylcholine receptor
Xin HUI; Jie GAO; Xin XIE; Naoki SUTO; Tsuyoshi OGIKU; Ming-Wei WANG
2005-01-01
Aim: To develop a homogeneous high-throughput screening (HTS) assay based on scintillation proximity assay (SPA) technology for identification of novel α4β2 nicotinic acetylcholine receptor (nAChR) modulators. Methods: Membrane preparation of HEK293 cells expressing α4β2 nAChR, [3H]cytisine and wheat germ agglutinin (WGA)-coupled microbeads were used to develop an HTS assay based on SPA technology. This method was validated against a conventional filter binding approach and applied to large-scale screening of a library containing 32 000 synthetic compounds. Intracellular calcium measurement was carried out to verify the bioactivities of the hits found by the SPA assay. Results: IC50 values of 2 reference compounds (epibatidine and RJR 2403) determined by SPA and filter binding methods were comparable and consistent with those reported elsewhere. A total of 54 compounds, showing more than 60% competitive inhibition on [3H]cytisine binding to α4β2 nAChR, were identified initially following an HTS campaign. Secondary screening confirmed that 17 compounds with novel chemical structures possessed relatively high binding affinity to α4β2 nAChR (Ki＜2 μmol/L). Eight compounds displayed antagonistic effects with ＞50% inhibition on ABT-594-induced calcium mobilization while none showed any agonist activity. Conclusions: This homogeneous binding assay is a highly efficient,amenable to automation and robust tool to screen potential α4β2 nAChR modulators in an HTS setting. Its application may be expanded to other membrane receptors and ion channels.
Lundberg, Martin; Thorsen, Stine Buch; Assarsson, Erika;
2011-01-01
A high throughput protein biomarker discovery tool has been developed based on multiplexed proximity ligation assays (PLA) in a homogeneous format in the sense of no washing steps. The platform consists of four 24-plex panels profiling 74 putative biomarkers with sub pM sensitivity each consuming...... only 1 micro Litre of human plasma sample. The system uses either matched monoclonal antibody pairs or the more readily available single batches of affinity purified polyclonal antibodies to generate the target specific reagents by covalently linking with unique nucleic acid sequences. These paired...
Dennis, T.; Scatton, B. (Synthelabo-L.E.R.S., Bagneux (France))
1982-11-01
A simple, sensitive and specific radioenzymatic assay for the measurement of 3,4-dihydroxyphenylethyleneglycol (DOPEG) was developed. The assay is based on the conversion of the compound to its O-methylated derivative in the presence of catechol-O-methyltransferase and (/sup 3/H)S-adenosyl-methionine. The tritiated 3-methoxy-4-hydroxyphenylethyleneglycol formed is selectively extracted in organic solvents and isolated by thin layer chromatography. After oxidation to vanillin the O-methylated compound is extracted and measured by liquid scintillation spectrophotometry. This assay has been applied to the measurement of free and conjugated DOPEG is a variety of biological tissues and fluids. Both free and conjugated DOPEG were readily detected in discrete rat brain areas. Substantial amounts of free and conjugated DOPEG were also measured in ventricular perfusates from freely moving rats. Finally, the presence of DOPEG was also demonstrated in human cerebrospinal fluid, plasma and urine. Only the free form of DOPEG was found in cerebrospinal fluid, whereas both unconjugated and conjugated forms were present in plasma and urine.
Homogeneous plate based antibody internalization assay using pH sensor fluorescent dye.
Nath, Nidhi; Godat, Becky; Zimprich, Chad; Dwight, Stephen J; Corona, Cesear; McDougall, Mark; Urh, Marjeta
2016-04-01
Receptor-mediated antibody internalization is a key mechanism underlying several anti-cancer antibody therapeutics. Delivering highly toxic drugs to cancer cells, as in the case of antibody drug conjugates (ADCs), efficient removal of surface receptors from cancer cells and changing the pharmacokinetics profile of the antibody drugs are some of key ways that internalization impacts the therapeutic efficacy of the antibodies. Over the years, several techniques have been used to study antibody internalization including radiolabels, fluorescent microscopy, flow cytometry and cellular toxicity assays. While these methods allow analysis of internalization, they have limitations including a multistep process and limited throughput and are generally endpoint assays. Here, we present a new homogeneous method that enables time and concentration dependent measurements of antibody internalization. The method uses a new hydrophilic and bright pH sensor dye (pHAb dye), which is not fluorescent at neutral pH but becomes highly fluorescent at acidic pH. For receptor mediated antibody internalization studies, antibodies against receptors are conjugated with the pHAb dye and incubated with the cells expressing the receptors. Upon binding to the receptor, the dyes conjugated to the antibody are not fluorescent because of the neutral pH of the media, but upon internalization and trafficking into endosomal and lysosomal vesicles the pH drops and dyes become fluorescent. The enabling attributes of the pHAb dyes are the hydrophilic nature to minimize antibody aggregation and bright fluorescence at acidic pH which allows development of simple plate based assays using a fluorescent reader. Using two different therapeutic antibodies--Trastuzumab (anti-HER2) and Cetuximab (anti-EGFR)--we show labeling with pHAb dye using amine and thiol chemistries and impact of chemistry and dye to antibody ration on internalization. We finally present two new approaches using the pHAb dye, which will be
Sensitive detection of p53 antibodies in a homogeneous fluorescence assay format
Neuweiler, Hannes; Schulz, Andreas; Wolfrum, Juergen M.; Sauer, Markus
2002-06-01
Circulating p53 autoantibodies are found to be a universal and highly specific tumor marker for malignant diseases. Hence, sereological screening for p53 autoantibodies at low concentration levels has become increasingly relevant for early-stage and follow-up of tumor diagnostics. We developed a new method for the highly sensitive detection of p53 antibodies in a homogeneous fluorescence assay format. Short, linear peptide derived form antibody recognition sequences so human p53 were labeled with an oxazine dye. Hydrophobic interactions constrain a conformation, where the dye interacts selectively with a tryptophan residue in the peptide sequence. Subsequently, the fluorescence of the dye is quenched efficiently due to electron transfer from the indole derivative to the dye in the excited state. Specific antibody recognition induces a conformational change in the peptide structure, repealing the dye-tryptophan interaction. Consequently, a fluorescence increase upon antibody binding signals the binding event. The long-wavelength absorption and emission characteristics of the probe and the use of a red pulsed diode laser as excitation source in a confocal fluorescence microscopic set-up allows ultra sensitive antibody detection at the single-molecule level. The effectiveness of the probes are highlighted by the detection of individual p53 autoantibodies directly in serum dilutions of cancer patients.
Ellen R. Goldman
2005-01-01
Full Text Available A homogeneous assay was used to detect 2,4,6-trinitrotoluene (TNT spiked into environmental water samples. This assay is based on changes in fluorescence emission intensity when TNT competitively displaces a fluorescently labeled, TNT analog bound to an anti-TNT antibody. The effectiveness of the assay was highly dependent on the source of the sample being tested. As no correlation between pH and assay performance was observed, ionic strength was assumed to be the reason for variation in assay results. Addition of 10x phosphate-buffered saline to samples to increase their ionic strength to that of our standard laboratory buffer (about 0.17 M significantly improved the range over which the assay functioned in several river water samples.
Mindy I Davis
Full Text Available Phosphoinositide kinases regulate diverse cellular functions and are important targets for therapeutic development for diseases, such as diabetes and cancer. Preparation of the lipid substrate is crucial for the development of a robust and miniaturizable lipid kinase assay. Enzymatic assays for phosphoinositide kinases often use lipid substrates prepared from lyophilized lipid preparations by sonication, which result in variability in the liposome size from preparation to preparation. Herein, we report a homogeneous 1536-well luciferase-coupled bioluminescence assay for PI5P4Kα. The substrate preparation is novel and allows the rapid production of a DMSO-containing substrate solution without the need for lengthy liposome preparation protocols, thus enabling the scale-up of this traditionally difficult type of assay. The Z'-factor value was greater than 0.7 for the PI5P4Kα assay, indicating its suitability for high-throughput screening applications. Tyrphostin AG-82 had been identified as an inhibitor of PI5P4Kα by assessing the degree of phospho transfer of γ-(32P-ATP to PI5P; its inhibitory activity against PI5P4Kα was confirmed in the present miniaturized assay. From a pilot screen of a library of bioactive compounds, another tyrphostin, I-OMe tyrphostin AG-538 (I-OMe-AG-538, was identified as an ATP-competitive inhibitor of PI5P4Kα with an IC(50 of 1 µM, affirming the suitability of the assay for inhibitor discovery campaigns. This homogeneous assay may apply to other lipid kinases and should help in the identification of leads for this class of enzymes by enabling high-throughput screening efforts.
Rui ZHANG; Pang-ke YAN; Cai-hong ZHOU; Jia-yu LIAO; Ming-wei WANG
2007-01-01
Aim: To develop homogeneous calcium mobilization assay for high-throughput screening (HTS) of mas-related gene (Mrg) receptor agonists. Methods: CHO-K1 cells stably expressing the full-length MrgD receptor and a calcium-sensitive dye were used to develop an HTS assay based on intracellular calcium influx. This method was applied to large-scale screening of a library containing 8000 synthetic compounds and natural product extracts, cAMP measurements were camed out to verify the bioactivities of the hits found by the calcium mobilization assay. Similar approaches were also employed in the identification of the MrgA1 recep-tor agonists following HTS of 16 000 samples. Results: EC50 values of the positive control compounds (β-alanine for MrgD receptor and dynorphin A for MrgA1 receptor) determined by the calcium mobilization assay were consistent with those reported in the literature, and the Z' factors were 0.65 and 0.50 for MrgD and MrgA1 receptor assay, respectively. About 31 compounds for the MrgD receptor and 48 compounds for the MrgA1 receptor showing ≥20% of the maximal agonist activities found in the controls were initially identified as hits. Secondary screen- ing confirmed that 2 compounds for each receptor possessed specific agonist activities. Intracellular cAMP level measurements indicated that the 2 confirmed hits displayed the functionality of the MrgD receptor agonists. Conclusion: A series of validation studies demonstrated that the homogeneous calcium mobili-zation assay developed was highly efficient, amenable to automation and a robust tool to screen potential MrgD and MrgA1 receptor agonists. Its application may be expanded to other G-protein coupled receptors that mobilize calcium influx upon activation.
Bauersfeld, W; Ratge, D; Knoll, E; Wisser, H
1986-03-01
The determination of norepinephrine and epinephrine in plasma by HPLC with amperometric detection was modified, giving detection limits of 25 ng/l for norepinephrine and epinephrine, respectively, using 1 ml plasma. In order to achieve this sensitivity, it was necessary to minimize the background noise by modification of instrumentation and specimen handling. Particularly important was the extra purification of the reagents, the application of micro-bore HPLC, the enzymatic cleavage of uric acid and temperature control of the amperometric cell and the amplifier. Comparison of the present method with the radioenzymatic determination of catecholamines resulted in coefficients of correlation of r = 0.924 and 0.919 for norepinephrine and epinephrine, resp. (n = 38). The concentrations of the 38 different samples used for the comparison were in the physiological range.
Nikiforov, Theo T; Beechem, Joseph M
2006-10-01
We studied the fluorescence resonance energy transfer (FRET) between quantum dots emitting at 565, 605, and 655 nm as energy donors and Alexa Fluor fluorophores with absorbance maxima at 594, 633, 647, and 680 nm as energy acceptors. As a first step, we prepared covalent conjugates between all three types of quantum dots and each of the Alexa Fluor fluorophores that could act as an energy acceptor. All of these conjugates displayed efficient resonance energy transfer. Then we prepared covalent conjugates of these quantum dots with biotin, fluorescein, and cortisol and established that the binding of these conjugates to suitable Alexa Fluor-labeled antibodies and streptavidin (in the case of biotin) can be efficiently detected by measuring the resonance energy transfer in homogeneous solutions. Finally, based on these observations, competitive binding assays for these three small analytes were developed. The performance of these assays as a function of the degree of labeling of the quantum dots was evaluated. It was found that decreasing the degree of loading of the quantum dots leads to decreases of the limits of detection. The results show the great potential of this FRET system for the development of new homogeneous binding assays.
Zscharnack, Kristin; Kreisig, Thomas; Prasse, Agneta A. [Institute of Bioanalytical Chemistry, Faculty of Chemistry and Mineralogy, Universität Leipzig, Deutscher Platz 5, 04103 Leipzig (Germany); Zuchner, Thole, E-mail: Thole.Zuechner@octapharma.com [Institute of Bioanalytical Chemistry, Faculty of Chemistry and Mineralogy, Universität Leipzig, Deutscher Platz 5, 04103 Leipzig (Germany); Center for Biotechnology and Biomedicine, Universität Leipzig, Deutscher Platz 5, 04103 Leipzig (Germany)
2015-01-07
Highlights: • Application of the TRF-based PATb system for universal oxidase substrate detection. • H{sub 2}O{sub 2} generated by choline or glucose oxidase quenches the TRF signal of PATb. • The assay time is only limited by the oxidase catalysis rate. • Glucose is precisely detected in human serum consistent to a commercial assay. • A reliable quantification of choline in infant formula is shown. - Abstract: H{sub 2}O{sub 2} is a widely occurring molecule which is also a byproduct of a number of enzymatic reactions. It can therefore be used to quantify the corresponding enzymatic substrates. In this study, the time-resolved fluorescence emission of a previously described complex consisting of phthalic acid and terbium (III) ions (PATb) is used for H{sub 2}O{sub 2} detection. In detail, glucose oxidase and choline oxidase convert glucose and choline, respectively, to generate H{sub 2}O{sub 2} which acts as a quencher for the PATb complex. The response time of the PATb complex toward H{sub 2}O{sub 2} is immediate and the assay time only depends on the conversion rate of the enzymes involved. The PATb assay quantifies glucose in a linear range of 0.02–10 mmol L{sup −1}, and choline from 1.56 to 100 μmol L{sup −1} with a detection limit of 20 μmol L{sup −1} for glucose and 1.56 μmol L{sup −1} for choline. Both biomolecules glucose and choline could be detected without pretreatment with good precision and reproducibility in human serum samples and infant formula, respectively. Furthermore, it is shown that the detected glucose concentrations by the PATb system agree with the results of a commercially available assay. In principle, the PATb system is a universal and versatile tool for the quantification of any substrate and enzyme reaction where H{sub 2}O{sub 2} is involved.
Tan, Yafang; Sutanto, Erick; Alleyne, Andrew G; Cunningham, Brian T
2014-04-01
We demonstrate the enhancement of a liquid-based homogenous fluorescence assay using the resonant electric fields from a photonic crystal (PC) surface. Because evanescent fields are confined to the liquid volume nearest to the photonic crystal, we developed a simple approach for integrating a PC fabricated on a silicon substrate within a fluid channel with submicron height, using electrohydrodynamic jet (e-jet) printing of a light-curable epoxy adhesive to define the fluid channel pattern. The PC is excited by a custom-designed compact instrument that illuminates the PC with collimated light that precisely matches the resonant coupling condition when the PC is covered with aqueous media. Using a molecular beacon nucleic acid fluorescence resonant energy transfer (FRET) probe for a specific miRNA sequence, we demonstrate an 8× enhancement of the fluorescence emission signal, compared to performing the same assay without exciting resonance in the PC detecting a miRNA sequence at a concentration of 62 nM from a liquid volume of only ∼20 nL. The approach may be utilized for any liquid-based fluorescence assay for applications in point-of-care diagnostics, environmental monitoring, or pathogen detection.
Ebner, Andreas; Marek, Markus; Kaiser, Karl; Kada, Gerald; Hahn, Christoph D; Lackner, Bernd; Gruber, Hermann J
2008-01-01
Biotin-4-fluorescein (B4F) is a convenient molecular probe for (strept)avidin and for unlabeled biotin in homogeneous fluorescence assays. The primary standard is a 16 microM working solution of d-biotin which is used to titrate an aliquot of a (strept)avidin stock solution while monitoring the tryptophane fluorescence of (strept)avidin. This serves to standardize the (strept)avidin stock solution, an aliquot of which is then titrated with a roughly 16 microM working solution of B4F while monitoring the fluorescence of B4F. Specific binding is accompanied by quenching, but after saturation of all binding sites, the appearance of free ligand causes a sharp rise of intense fluorescence, the beginning of which allows to calculate the effective concentration of B4F in the working solution. Measurement of avidin in a crude sample is exemplified by mixing 8 pmol of B4F with various amounts of diluted egg white in a volume of 1 mL. Hereby, the extent of fluorescence quenching linearly correlates with the concentration of functional avidin. Moreover, a sharp minimum of fluorescence is observed when exactly 2 pmol of avidin is present in the sample. The latter assay has been adapted to measure between 0.5 and 5 pmol of d-biotin in 1 mL of sample by adding 1.9 pmol of avidin and 8 pmol of B4F. This competitive assay correctly measures the small dose of d-biotin in multivitamin tablets (e.g., 150 microg in 5 g of solid) after subtracting the background fluorescence of the colored aqueous solution.
Sasamoto, Kenta; Hirayama, Satoshi; Kon, Mika; Seino, Utako; Ueno, Tsuyoshi; Nagao, Yuki; Hirayama, Akiko; Isshiki, Miwa; Idei, Mayumi; Yano, Kouji; Miida, Takashi
2017-07-01
High-density lipoprotein-cholesterol (HDL-C) is generally measured using several homogeneous assays. We aimed to clarify whether apolipoprotein E-containing HDL (apoE-HDL) subfractions are altered during storage, and if so, whether such changes affect the HDL-C concentration measured using homogeneous assays. We stored serum from normolipidemic (n=32) and dyslipidemic (n=17) subjects at 4°C for up to 7days. ApoE-HDL subfractions were analyzed using native 2-dimensional gel (native 2D-gel) electrophoresis. HDL-C concentrations were determined using 2 precipitation and 4homogeneous assays. Native 2D-gel electrophoresis revealed variously sized apoE-HDL subfractions. After 4h incubation at 37°C, subfractions of smaller particles were converted into larger particles by lecithin:cholesterol acyltransferase (LCAT) activity. After 7days storage at 4°C, the smaller subfractions were decreased in the normolipidemic group, accompanying increases in larger subfractions, whereas changes in the respective subfractions varied among individuals in the dyslipidemic group. HDL-C concentrations were significantly lower after storage at 4°C in all assays, except that using Sekisui Medical's reagent. Therefore, changes in HDL-C concentration and apoE-HDL subfractions were independent of each other. ApoE-HDL subfractions change during storage, but these changes are not linked to those in HDL-C concentration measured using homogeneous assays. Copyright © 2017 Elsevier B.V. All rights reserved.
Lopez-Crapez, E; Bazin, H; Andre, E; Noletti, J; Grenier, J; Mathis, G
2001-07-15
Oligonucleotide ligation assay (OLA) is considered to be a very useful methodology for the detection and characterization of mutations, particularly for clinical purposes. The fluorescence resonance energy transfer between a fluorescent donor and a suitable fluorophore as acceptor has been applied in the past to several scientific fields. This technique is well adapted to nucleic acid analysis such as DNA sequencing, DNA hybridization and polymerase chain reaction. We describe here a homogeneous format based on the use of a rare earth cryptate label as donor: tris-bipyridine-Eu(3+). The long-lived fluorescence of this label makes it possible to reach a high sensitivity by using a time-resolved detection mode. A non-radiative energy transfer technology, known as time-resolved amplification of cryptate emission (TRACE((R))) characterized by a temporal and spectral selectivity has been developed. The TRACE((R)) detection of characterized single nucleotide polymorphism using the OLA for allelic discrimination is proposed. We demonstrate the potentialities of this OLA-TRACE((R)) methodology through the analysis of K-ras oncogene point mutations.
LIU Yan; XU Guo-bin
2010-01-01
criteria; Roche (Enzymatic) met the optimum criteria. The other five systems met the desirable criteria. Compared with the second criterion, all the results met the requirement of CLIA' 88. Trueness evaluation showed: the MEeGFR of Dade Behring exceeded 10% while the MEeGFRs of Beckman (traceable to rate Jaffe), Beckman (traceable to IDMS) and Ortho (traceable to Jaffe/High Pedormance Liquid Chromatography)exceeded 20% at level Ⅰ. At level Ⅱ the MEeGFRs of Dade Behring, Ortho (traceable to GC/IDMS) and Beckman (traceable to rate Jaffe) exceeded 10%. None of the nine systems got a MEeGFR higher than 20%. The conclusions of NIST SRM 967 agreed with those of LN 24 except for the Beckman measurement system.Conclusions Trueness investigation of routine creatinine assays on nine homogeneous systems demonstrates an encouraging outcome that meets clinical requirements. Among the nine homogeneous routine systems, Roche and Daiichi produce the most accurate results. The implementation of traceability is effective.
Miners, J.S.; Verbeek, M.M.; Olde Rikkert, M.G.M.; Kehoe, P.G.; Love, S.
2008-01-01
Neprilysin, a zinc-metalloendopeptidase, has important roles in the physiology and pathology of many diseases such as hypertension, cancer and Alzheimer's disease. We have developed an immunocapture assay to measure the specific enzyme activity of neprilysin in brain tissue homogenates and
Kiss, László; Cselenyák, Attila; Varga, Ágnes; Visegrády, András
2016-08-15
Cell-based assays for G-protein-coupled receptor (GPCR) activation applied in high-throughput screening (HTS) monitor various readouts for second messengers or intracellular effectors. Recently, our understanding of diverging signaling pathways downstream of receptor activation and the capability of small molecules to selectively modulate signaling routes has increased substantially, underlining the importance of selecting appropriate readouts in cellular functional screens. To minimize the rate of false negatives in large-scale screening campaigns, it is crucial to maximize the chance of a ligand being detected, and generally applicable methods for detecting multiple analytes from a single well might serve this purpose. The few assays developed so far based on multiplexed GPCR readouts are limited to only certain applications and usually rely on genetic manipulations hindering screening in native or native-like cellular systems. Here we describe a more generally applicable and HTS-compatible homogeneous assay based on the combination of fluorometric detection of [Ca(2+)] with subsequent homogeneous time-resolved fluorescence (HTRF) cAMP readout in the same well. Besides describing development and validation of the assay, using a cell line recombinantly expressing the human PTH1 receptor screening of a small library is also presented, demonstrating the robustness and HTS compatibility of the novel paradigm.
Cerny, Matthew A; Csengery, Alexander; Schmenk, Jennifer; Frederick, Kosea
2015-11-01
Elevated levels of aldosterone are associated with arterial hypertension, congestive heart failure, chronic kidney disease, and obesity. Aldosterone is produced predominantly in the zona glomerulosa of the cortex of the adrenal gland by the enzyme aldosterone synthase (CYP11B2). Treatment of the above indications by decreasing production of aldosterone is thought to be of therapeutic benefit by lessening the deleterious effects of aldosterone mediated through both the mineralocorticoid receptor and also through so called non-genomic pathways. However, inhibition of the highly similar enzyme, CYP11B1, which is responsible for the production of cortisol, must be avoided in the development of clinically useful aldosterone synthase inhibitors due to the resulting impairment of the cortisol-induced stress response. In efforts to assess the interactions of compounds with the CYP11B enzymes, a variety of cell-based inhibitor screening assays for both CYP11B1 and CYP11B2 have been reported. Herein we report details of assays employing both cynomolgus monkey adrenal homogenate (CAH) and human adrenal homogenate (HAH) as sources of CYP11B1 and CYP11B2 enzymes. Utilizing both CAH and HAH, we have characterized the kinetics of the CYP11B1-mediated conversion of 11-deoxycortisol to cortisol and the CYP11B2-mediated oxidation of corticosterone to aldosterone. Inhibition assays for both CYP11B1 and CYP11B2 were subsequently developed. Based on a comparison of human and monkey amino acid sequences, kinetics data, and inhibition values derived from the HAH and CAH assays, evidence is provided in support of using cynomolgus monkey tissue-derived cell homogenates as suitable surrogates for the human enzymes.
Ikeda, Tomohiro; Miyao, Hiroki; Sueda, Shinji
2014-06-17
Biotin is a water-soluble vitamin serving as a cofactor for several metabolic enzymes and plays crucial roles in every living cell. In the present study, we describe a noncompetitive assay for determination of biotin in a homogeneous solution. Our assay is based on a biotinylation reaction from archaeon Sulfolobus tokodaii. S. tokodaii biotinylation has a unique property that biotin protein ligase (BPL) forms a stable complex with its biotinylated substrate protein (BCCP). Determination of biotin was performed by monitoring the complexation reaction between BPL and BCCP through biotinylation, based on luminescence resonance energy transfer (LRET) from a Tb(3+) complex to fluorescein, where BPL and BCCP were labeled with a Tb(3+) complex and fluorescein, respectively. Our assay allows for ultrasensitive detection of biotin with a detection limit of approximately 1 pM (or 0.2 fmol in a 0.2 mL sample volume) by a simple procedure without use of radioactive materials or enzymatic signal amplification. In addition, owing to its noncompetitive format, our assay has a very wide measurement range of at least 3 orders of magnitude. Our assay is also beneficial as a model system for interaction analysis based on LRET.
Reija, Belén; Monterroso, Begoña; Jiménez, Mercedes; Vicente, Miguel; Rivas, Germán; Zorrilla, Silvia
2011-11-01
We present here a fluorescence anisotropy method for the quantification of the polymerization of FtsZ, an essential protein for cytokinesis in prokaryotes whose GTP-dependent assembly initiates the formation of the divisome complex. Using Alexa 488 labeled wild-type FtsZ as a tracer, the assay allows determination of the critical concentration of FtsZ polymerization from the dependence of the measured steady-state fluorescence anisotropy on the concentration of FtsZ. The incorporation of the labeled protein into FtsZ polymers and the lack of spectral changes on assembly were independently confirmed by time-resolved fluorescence and fluorescence correlation spectroscopy. Critical concentration values determined by this new assay are compatible with those reported previously under the same conditions by other well-established methods. As a proof of principle, data on the sensitivity of the assay to changes in FtsZ assembly in response to Mg(2+) concentration or to the presence of high concentrations of Ficoll 70 as crowding agent are shown. The proposed method is sensitive, low sample consuming, rapid, and reliable, and it can be extended to other cooperatively polymerizing systems. In addition, it can help to discover new antimicrobials that may interfere with FtsZ polymerization because it can be easily adapted to systematic screening assays.
Hou, Ting; Li, Wei; Liu, Xiaojuan; Li, Feng
2015-11-17
Homogenous electrochemical biosensing strategies have attracted substantial attention, because of their advantages of being immobilization-free and having rapid response and improved recognition efficiency, compared to heterogeneous biosensors; however, the high cost of labeling and the strict reaction conditions of tool enzymes associated with current homogeneous electrochemical methods limit their potential applications. To address these issues, herein we reported, for the first time, a simple label-free and enzyme-free homogeneous electrochemical strategy based on hybridization chain reaction (HCR) for sensitive and highly specific detection of microRNA (miRNA). The target miRNA triggers the HCR of two species of metastable DNA hairpin probes, resulting in the formation of multiple G-quadruplex-incorporated long duplex DNA chains. Thus, with the electrochemical indicator Methylene Blue (MB) selectively intercalated into the duplex DNA chain and the multiple G-quadruplexes, a significant electrochemical signal drop is observed, which is dependent on the concentration of the target miRNA. Thus, using this "signal-off" mode, a simple, label-free and enzyme-free homogeneous electrochemical strategy for sensitive miRNA assay is readily realized. This strategy also exhibits excellent selectivity to distinguish even single-base mismatched miRNA. Furthermore, this method also exhibits additional advantages of simplicity and low cost, since both expensive labeling and sophisticated probe immobilization processes are avoided. Therefore, the as-proposed label-free and enzyme-free homogeneous electrochemical strategy may become an alternative method for simple, sensitive, and selective miRNA detection, and it has great potential to be applied in miRNA-related clinical diagnostics and biochemical research.
Kopra, Kari; Ligabue, Alessio; Wang, Qi; Syrjänpää, Markku; Blaževitš, Olga; Veltel, Stefan; van Adrichem, Arjan J; Hänninen, Pekka; Abankwa, Daniel; Härmä, Harri
2014-07-01
A quenching resonance energy transfer (QRET) assay for small GTPase nucleotide exchange kinetic monitoring is demonstrated using nanomolar protein concentrations. Small GTPases are central signaling proteins in all eukaryotic cells acting as a "molecular switches" that are active in the GTP-state and inactive in the GDP-state. GTP-loading is highly regulated by guanine nucleotide exchange factors (GEFs). In several diseases, most prominently cancer, this process in misregulated. The kinetics of the nucleotide exchange reaction reports on the enzymatic activity of the GEF reaction system and is, therefore, of special interest. We determined the nucleotide exchange kinetics using europium-labeled GTP (Eu-GTP) in the QRET assay for small GTPases. After GEF catalyzed GTP-loading of a GTPase, a high time-resolved luminescence signal was found to be associated with GTPase bound Eu-GTP, whereas the non-bound Eu-GTP fraction was quenched by soluble quencher. The association kinetics of the Eu-GTP was measured after GEF addition, whereas the dissociation kinetics could be determined after addition of unlabeled GTP. The resulting association and dissociation rates were in agreement with previously published values for H-Ras(Wt), H-Ras(Q61G), and K-Ras(Wt), respectively. The broader applicability of the QRET assay for small GTPases was demonstrated by determining the kinetics of the Ect2 catalyzed RhoA(Wt) GTP-loading. The QRET assay allows the use of nanomolar protein concentrations, as more than 3-fold signal-to-background ratio was achieved with 50 nM GTPase and GEF proteins. Thus, small GTPase exchange kinetics can be efficiently determined in a HTS compatible 384-well plate format.
Saikawa Shinichi
2008-05-01
Full Text Available Abstract Background Increased serum remnant lipoproteins are supposed to predict cardiovascular disease in addition to increased LDL. A new homogenous assay for remnant lipoprotein-cholesterol (RemL-C has been developed as an alternative to remnant-like particle-cholesterol (RLP-C, an immunoseparation assay, widely used for the measurement of remnant lipoprotein cholesterol. Methods We evaluated the correlations and data validation between the 2 assays in 83 subjects (49 men and 34 women without diabetes, hypertension and medications for hyperlipidemia, diabetes, and hypertension, and investigated the characteristics of remnant lipoproteins obtained by the two methods (RLP-C and RemL-C and their relationships with IDL-cholesterol determined by our developed HPLC method. Results A positive correlation was significantly found between the two methods (r = 0.853, 95%CI 0.781–0.903, p RLP-C level. RemL-C (r = 0.339, 95%CI 0.152–0.903; p = 0.0005 significantly correlated with IDL-cholesterol, but not RLP-C (r = 0.17, 95%CI -0.047–0.372; p = 0.1237 in all the samples (n = 83. Conclusion These results suggest that there is generally a significant correlation between RemL-C and RLP-C. However, RemL-C assay is likely to reflect IDL more closely than RLP-C.
Mohammed Akli eAyoub
2014-06-01
Full Text Available The extracellular signal-regulated kinases (ERKs are key components of multiple important cell signalling pathways regulating diverse biological responses. This signalling is characterized by phosphorylation cascades leading to ERK1/2 activation and promoted by various cell surface receptors including G protein-coupled receptors (GPCRs and receptor tyrosine kinases (RTKs. We report the development of a new cell-based phospho-ERK1/2 assay (designated Phospho-ERK, which is a sandwich proximity-based assay using the homogeneous time-resolved fluorescence technology. We have validated the assay on endogenously expressed ERK1/2 activated by the epidermal growth factor (EGFR as a prototypical RTK, as well as various GPCRs belonging to different classes and coupling to different heterotrimeric G proteins. The assay was successfully miniaturized in 384-well plates using various cell lines endogenously, transiently or stably expressing the different receptors. The validation was performed for agonists, antagonists and inhibitors in dose-response as well as kinetic analysis, and the signalling and pharmacological properties of the different receptors were reproduced. Furthermore, the determination of a Z’-factor value of 0.7 indicates the potential of the Phospho-ERK assay for high-throughput screening of compounds that may modulate ERK1/2 signalling. Finally, our study is of great interest in the current context of investigating ERK1/2 signalling with respect to the emerging concepts of biased ligands, G protein-dependent/independent ERK1/2 activation, and functional transactivation between GPCRs and RTKs, illustrating the importance of considering the ERK1/2 pathway in cell signalling.
Titus, Steven A; Beacham, Daniel; Shahane, Sampada A; Southall, Noel; Xia, Menghang; Huang, Ruili; Hooten, Elizabeth; Zhao, Yong; Shou, Louie; Austin, Christopher P; Zheng, Wei
2009-11-01
Long QT syndrome, either inherited or acquired from drug treatments, can result in ventricular arrhythmia (torsade de pointes) and sudden death. Human ether-a-go-go-related gene (hERG) channel inhibition by drugs is now recognized as a common reason for the acquired form of long QT syndrome. It has been reported that more than 100 known drugs inhibit the activity of the hERG channel. Since 1997, several drugs have been withdrawn from the market due to the long QT syndrome caused by hERG inhibition. Food and Drug Administration regulations now require safety data on hERG channels for investigative new drug (IND) applications. The assessment of compound activity on the hERG channel has now become an important part of the safety evaluation in the process of drug discovery. During the past decade, several in vitro assay methods have been developed and significant resources have been used to characterize hERG channel activities. However, evaluation of compound activities on hERG have not been performed for large compound collections due to technical difficulty, lack of throughput, and/or lack of biological relevance to function. Here we report a modified form of the FluxOR thallium flux assay, capable of measuring hERG activity in a homogeneous 1536-well plate format. To validate the assay, we screened a 7-point dilution series of the LOPAC 1280 library collection and reported rank order potencies of ten common hERG inhibitors. A correlation was also observed for the hERG channel activities of 10 known hERG inhibitors determined in this thallium flux assay and in the patch clamp experiment. Our findings indicate that this thallium flux assay can be used as an alternative method to profile large-volume compound libraries for compound activity on the hERG channel.
Wang, Shui-Long; Ohrmund, Linda; Hauenstein, Scott; Salbato, Jared; Reddy, Rukmini; Monk, Patrick; Lockton, Steven; Ling, Nicholas; Singh, Sharat
2012-08-31
Antibody-based drugs such as infliximab (IFX) are effective for the treatment of inflammatory bowel disease (IBD) and other immune-mediated disorders. The development of antibodies against these drugs may result in unfavorable consequences, including the loss of drug efficacy, hypersensitivity reactions, and other adverse events. Therefore, accurate monitoring of serum drug and anti-drug antibody levels should be an important part of therapy for patients being treated with an antibody-based drug. Current methods for the assessment of anti-drug antibodies and drug levels, involving various bridging ELISA and radioimmunoassay techniques, are limited by their sensitivity, interference, and/or complexity. To overcome these limitations, we have developed a non-radiolabeled homogeneous mobility shift assay (HMSA) to measure the antibodies-to-infliximab (ATI) and IFX levels in serum samples. Full method validation was performed on both the ATI- and IFX-HMSA, and the clinical sample test results were also compared with those obtained from a bridging ELISA method to evaluate the difference in performance between the two assays. Validation of the ATI-HMSA revealed a lower limit of quantitation of 0.012 μg/mL in serum. The linear range of quantitation was 0.029-0.54 μg/mL. The intra- and inter-assay precision was less than 20% of coefficient of variation (CV), and the accuracy (% error) of the assay was less than 20%. In serum samples, ATI as low as 0.036 μg/mL can be measured, even in the presence of 60 μg/mL of IFX in the serum. Sera from 100 healthy subjects were tested to determine the cut point of the assay. ATI-positive samples that had been previously analyzed by using a bridging ELISA from 100 patients were also measured by the new method. There was a high correlation between the two methods for ATI levels (pELISA method. Validation of the mobility shift IFX assay also showed high assay sensitivity, precision and accuracy. The HMSA method may also be applied to
赵芳; 岳振峰; 吕敬章; 洪丹漩; 张恒; 刘慧玲; 黄欣迪; 洪小柳
2014-01-01
目的：采用纳米均相时间分辨荧光免疫技术(amplified luminescent proximity homogeneous assay, Alpha-LISA)建立高灵敏检测盐酸克伦特罗(clenbuterol, CLB)残留的方法。方法在发光微粒上固定 CLB,与游离的CLB共同竞争固定在感光微粒上的CLB单克隆抗体,优化检测条件并进行方法学考核。结果该方法的灵敏度为0.04 ng/mL。猪肉样品回收率在83%~109%之间,牛肉样品回收率在92%~112%之间；猪肉样品检测的批内变异系数CV<10%、批间变异系数CV<15%。与特布他林、沙丁胺醇的交叉反应率分别为34.5%、25.7%,与莱克多巴胺、妥布特罗、齐帕特罗无交叉反应。结论 CLB-AlphaLISA 检测肉类中克伦特罗残留具有简单、快速、灵敏性高、特异性强、稳定等特点,具有广阔的应用前景。%Objective A direct competitive amplified luminescent proximity homogeneous assay (Alpha-LISA) for detecting clenbuterol(CLB) was established. Methods CLB was coated on emitting particles, and competed with sample CLB to anti-CLB which was coated on photosensitive particles. The analytical perfor-mance and optinal test conditions of the method were studied. Results The sensitivity of the assay was 0.04 ng/mL. The recoveries of the determination for CLB in pork and beer were respectively 83%~109% and 92%~112%. The CV of intra-and inter-assay were <10% and <15% respectively. The cross-reactivity of the CLB-AlphaLISA with ractopamine, tulobuterol and zilpaterol was negligible, while that with terbutaline and salbutamol was 34.5% and 25.7% respectively. Conclusion The CLB-AlphaLISA had the characteristics of excellent specificity and sensitivity, and good precision and economy. It could be widely used in rapid screen-ing for CLB contamination in meat or foods in the future.
Miao, Yang-Bao [State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211 (China); Ren, Hong-Xia [Key Laboratory of Asymmetric Synthesis and Chirotechnology of Sichuan Province, Chengdu Institute of Organic Chemistry, Chinese Academy of Sciences, Chengdu 610041 (China); University of Chinese Academy of Sciences, Beijing 10049 (China); Gan, Ning, E-mail: ganning@nbu.edu.cn [State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211 (China); Zhou, You [State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211 (China); Cao, Yuting, E-mail: caoyuting@nbu.edu.cn [State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211 (China); Li, Tianhua [State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211 (China); Chen, Yinji [Faculty of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210000 (China)
2016-07-27
In this work, a novel homogeneous and signal “off–on” aptamer based fluorescence assay was successfully developed to detect chloramphenicol (CAP) residues in food based on the fluorescence resonance energy transfer (FRET). The vesicle nanotracer was prepared through labeling single stranded DNA binding protein (SSB) on limposome-CdSe/ZnS quantum dot (SSB/L-QD) complexes. It was worth mentioning that the signal tracer (SSB/L-QD) with vesicle shape, which was fabricated being encapsulated with a number of quantum dots and SSB. The nanotracer has excellent signal amplification effects. The vesicle composite probe was formed by combining aptamer labeled nano-gold (Au-Apt) and SSB/L-QD. Which based on SSB's specific affinity towards aptamer. This probe can't emit fluoresce which is in “off” state because the signal from SSB/L-QD as donor can be quenched by the Au-aptas acceptor. When CAP was added in the composite probe solution, the aptamer on the Au-Apt can be preferentially bounded with CAP then release from the composite probe, which can turn the “off” signal of SSB/L-QD tracer into “on” state. The assay indicates excellent linear response to CAP from 0.001 nM to 10 nM and detection limit down to 0.3 pM. The vesicle probes with size of 88 nm have strong signal amplification. Because a larger number of QDs can be labeled inside the double phosphorus lipid membrane. Besides, it was employed to detect CAP residues in the milk samples with results being agreed well with those from ELISA, verifying its accuracy and reliability. - Highlights: • Homogeneous and “off–on” fluorescence aptamer-based assay was developed to detect chloramphenicol (CAP) residues in food. • This probe was fabricated based on a vesicle QDs signal tracer (SSB/L-QD) combining with Au-Aptamer. • The detection mechanism was based on FRET with high specificity. • The results for CAP detection in the milk samples agreed well with those from ELISA, while
Qiu, Xue; Hildebrandt, Niko
2016-03-01
The importance of microRNA (miRNA) dysregulation in the development and progression of diseases has made these short-length nucleic acids to next generation biomarkers. Tb-to-QD Förster resonance energy transfer (FRET) has several unique advantages over organic dye-based FRET systems for biomolecular sensing. Large Förster distances (6-11 nm) offer much high FRET efficiencies, exceptionally long Tb excited-state lifetimes (ms) enable time-gated detection void of autofluorecence background, and the narrow, symmetric, and tunable emission bands of QDs provide unrivaled potential for multiplexing. Here we report a rapid and homogeneous method to sensitively detect three different miRNAs (hsa-miR-20a-5p, hsa-miR-20b-5p, and hsa-miR-21-5p) from a single 150 µL sample based on multiplexed FRET between a luminescent Lumi4-Tb complex and three different QDs. The biosensing approach exploits both base pairing and stacking. Careful design and optimization of sequence lengths and orientations of the QD and Tb-DNA conjugates was performed to provide maximum selectivity and sensitivity for all three miRNA biomarkers. The assays work at room temperature and were designed for their application on a KRYPTOR diagnostic plate reader system.Only 30 min of sample incubation and 7.5 s of measurement are required to obtain ca. 1 nM (subpicomol) detection limits. We also demonstrate precise multiplexed measurements of these miRNAs at different and varying concentrations and the feasibility of adapting the technology to point-of-care testing (POCT) in buffer containing 10% serum. Our assay does not only demonstrate an important milestone for the integration of quantum dots to multiplexed clinical diagnostics but also a unique rapid miRNA detection technology that is complimentary to the rather complicated high-throughput and high-sensitivity approaches that are established today.
A quantitative polymerase chain reaction (qPCR) assay was developed for the detection and quantification of Edwardsiella ictaluri in channel catfish Ictalurus punctatus pond water using modifications to a published E. ictaluri–specific qPCR assay and previously established protocols for the molecula...
Shapiro, Adam B; Livchak, Stephania; Gao, Ning; Whiteaker, James; Thresher, Jason; Jahić, Haris; Huang, Jian; Gu, Rong-Fang
2012-03-01
A novel assay for the NADPH-dependent bacterial enzyme UDP-N-acetylenolpyruvylglucosamine reductase (MurB) is described that has nanomolar sensitivity for product formation and is suitable for high-throughput applications. MurB catalyzes an essential cytoplasmic step in the synthesis of peptidoglycan for the bacterial cell wall, reduction of UDP-N-acetylenolpyruvylglucosamine to UDP-N-acetylmuramic acid (UNAM). Interruption of this biosynthetic pathway leads to cell death, making MurB an attractive target for antibacterial drug discovery. In the new assay, the UNAM product of the MurB reaction is ligated to L-alanine by the next enzyme in the peptidoglycan biosynthesis pathway, MurC, resulting in hydrolysis of adenosine triphosphate (ATP) to adenosine diphosphate (ADP). The ADP is detected with nanomolar sensitivity by converting it to oligomeric RNA with polynucleotide phosphorylase and detecting the oligomeric RNA with a fluorescent dye. The product sensitivity of the new assay is 1000-fold greater than that of the standard assay that follows the absorbance decrease resulting from the conversion of NADPH to NADP(+). This sensitivity allows inhibitor screening to be performed at the low substrate concentrations needed to make the assay sensitive to competitive inhibition of MurB.
Chadwick, John C; Freixa, Zoraida; van Leeuwen, Piet W N M
2011-01-01
This first book to illuminate this important aspect of chemical synthesis improves the lifetime of catalysts, thus reducing material and saving energy, costs and waste.The international panel of expert authors describes the studies that have been conducted concerning the way homogeneous catalysts decompose, and the differences between homogeneous and heterogeneous catalysts. The result is a ready reference for organic, catalytic, polymer and complex chemists, as well as those working in industry and with/on organometallics.
Sanchez, R.; Ragusa, J.; Santandrea, S. [Commissariat a l' Energie Atomique, Direction de l' Energie Nucleaire, Service d' Etudes de Reacteurs et de Modelisation Avancee, CEA de Saclay, DM2S/SERMA 91 191 Gif-sur-Yvette cedex (France)]. e-mail: richard.sanchez@cea.fr
2004-07-01
The problem of the determination of a homogeneous reflector that preserves a set of prescribed albedo is considered. Duality is used for a direct estimation of the derivatives needed in the iterative calculation of the optimal homogeneous cross sections. The calculation is based on the preservation of collapsed multigroup albedo obtained from detailed reference calculations and depends on the low-order operator used for core calculations. In this work we analyze diffusion and transport as low-order operators and argue that the P{sub 0} transfers are the best choice for the unknown cross sections to be adjusted. Numerical results illustrate the new approach for SP{sub N} core calculations. (Author)
Lifting locally homogeneous geometric structures
McKay, Benjamin
2011-01-01
We prove that under some purely algebraic conditions every locally homogeneous structure modelled on some homogeneous space is induced by a locally homogeneous structure modelled on a different homogeneous space.
Functionality and homogeneity.
2011-01-01
Functionality and homogeneity are two of the five Sustainable Safety principles. The functionality principle aims for roads to have but one exclusive function and distinguishes between traffic function (flow) and access function (residence). The homogeneity principle aims at differences in mass, spe
Functionality and homogeneity.
2011-01-01
Functionality and homogeneity are two of the five Sustainable Safety principles. The functionality principle aims for roads to have but one exclusive function and distinguishes between traffic function (flow) and access function (residence). The homogeneity principle aims at differences in mass, spe
Homogeneity of Inorganic Glasses
Jensen, Martin; Zhang, L.; Keding, Ralf;
2011-01-01
Homogeneity of glasses is a key factor determining their physical and chemical properties and overall quality. However, quantification of the homogeneity of a variety of glasses is still a challenge for glass scientists and technologists. Here, we show a simple approach by which the homogeneity...... of different glass products can be quantified and ranked. This approach is based on determination of both the optical intensity and dimension of the striations in glasses. These two characteristic values areobtained using the image processing method established recently. The logarithmic ratio between...... the dimension and the intensity is used to quantify and rank the homogeneity of glass products. Compared with the refractive index method, the image processing method has a wider detection range and a lower statistical uncertainty....
Homogeneity of Inorganic Glasses
Jensen, Martin; Zhang, L.; Keding, Ralf
2011-01-01
Homogeneity of glasses is a key factor determining their physical and chemical properties and overall quality. However, quantification of the homogeneity of a variety of glasses is still a challenge for glass scientists and technologists. Here, we show a simple approach by which the homogeneity...... of different glass products can be quantified and ranked. This approach is based on determination of both the optical intensity and dimension of the striations in glasses. These two characteristic values areobtained using the image processing method established recently. The logarithmic ratio between...... the dimension and the intensity is used to quantify and rank the homogeneity of glass products. Compared with the refractive index method, the image processing method has a wider detection range and a lower statistical uncertainty....
Benchmarking monthly homogenization algorithms
V. K. C. Venema
2011-08-01
Full Text Available The COST (European Cooperation in Science and Technology Action ES0601: Advances in homogenization methods of climate series: an integrated approach (HOME has executed a blind intercomparison and validation study for monthly homogenization algorithms. Time series of monthly temperature and precipitation were evaluated because of their importance for climate studies and because they represent two important types of statistics (additive and multiplicative. The algorithms were validated against a realistic benchmark dataset. The benchmark contains real inhomogeneous data as well as simulated data with inserted inhomogeneities. Random break-type inhomogeneities were added to the simulated datasets modeled as a Poisson process with normally distributed breakpoint sizes. To approximate real world conditions, breaks were introduced that occur simultaneously in multiple station series within a simulated network of station data. The simulated time series also contained outliers, missing data periods and local station trends. Further, a stochastic nonlinear global (network-wide trend was added.
Participants provided 25 separate homogenized contributions as part of the blind study as well as 22 additional solutions submitted after the details of the imposed inhomogeneities were revealed. These homogenized datasets were assessed by a number of performance metrics including (i the centered root mean square error relative to the true homogeneous value at various averaging scales, (ii the error in linear trend estimates and (iii traditional contingency skill scores. The metrics were computed both using the individual station series as well as the network average regional series. The performance of the contributions depends significantly on the error metric considered. Contingency scores by themselves are not very informative. Although relative homogenization algorithms typically improve the homogeneity of temperature data, only the best ones improve
Dynamics of homogeneous nucleation
Toxværd, Søren
2015-01-01
The classical nucleation theory for homogeneous nucleation is formulated as a theory for a density fluctuation in a supersaturated gas at a given temperature. But molecular dynamics simulations reveal that it is small cold clusters which initiates the nucleation. The temperature in the nucleating...
Tignanelli, H. L.; Vazquez, R. A.; Mostaccio, C.; Gordillo, S.; Plastino, A.
1990-11-01
RESUMEN. Presentamos una metodologia de analisis de la homogeneidad a partir de la Teoria de la Informaci6n, aplicable a muestras de datos observacionales. ABSTRACT:Standard concepts that underlie Information Theory are employed in order design a methodology that enables one to analyze the homogeneity of a given data sample. Key : DATA ANALYSIS
Dynamics of homogeneous nucleation
Toxværd, Søren
2015-01-01
The classical nucleation theory for homogeneous nucleation is formulated as a theory for a density fluctuation in a supersaturated gas at a given temperature. But molecular dynamics simulations reveal that it is small cold clusters which initiates the nucleation. The temperature in the nucleating...... clusters fluctuates, but the mean temperature remains below the temperature in the supersaturated gas until they reach the critical nucleation size. The critical nuclei have, however, a temperature equal to the supersaturated gas. The kinetics of homogeneous nucleation is not only caused by a grow...... or shrink by accretion or evaporation of monomers only but also by an exponentially declining change in cluster size per time step equal to the cluster distribution in the supersaturated gas....
Homogeneous group, research, institution
Francesca Natascia Vasta
2014-09-01
Full Text Available The work outlines the complex connection among empiric research, therapeutic programs and host institution. It is considered the current research state in Italy. Italian research field is analyzed and critic data are outlined: lack of results regarding both the therapeutic processes and the effectiveness of eating disorders group analytic treatment. The work investigates on an eating disorders homogeneous group, led into an eating disorder outpatient service. First we present the methodological steps the research is based on including the strong connection among theory and clinical tools. Secondly clinical tools are described and the results commented. Finally, our results suggest the necessity of validating some more specifical hypothesis: verifying the relationship between clinical improvement (sense of exclusion and painful emotions reduction and specific group therapeutic processes; verifying the relationship between depressive feelings, relapses and transition trough a more differentiated groupal field.Keywords: Homogeneous group; Eating disorders; Institutional field; Therapeutic outcome
Homogenous finitary symmetric groups
Otto. H. Kegel
2015-03-01
Full Text Available We characterize strictly diagonal type of embeddings of finitary symmetric groups in terms of cardinality and the characteristic. Namely, we prove the following. Let kappa be an infinite cardinal. If G=underseti=1stackrelinftybigcupG i , where G i =FSym(kappan i , (H=underseti=1stackrelinftybigcupH i , where H i =Alt(kappan i , is a group of strictly diagonal type and xi=(p 1 ,p 2 ,ldots is an infinite sequence of primes, then G is isomorphic to the homogenous finitary symmetric group FSym(kappa(xi (H is isomorphic to the homogenous alternating group Alt(kappa(xi , where n 0 =1,n i =p 1 p 2 ldotsp i .
Homogenization of resonant chiral metamaterials
Andryieuski, Andrei; Menzel, C.; Rockstuhl, Carsten
2010-01-01
Homogenization of metamaterials is a crucial issue as it allows to describe their optical response in terms of effective wave parameters as, e.g., propagation constants. In this paper we consider the possible homogenization of chiral metamaterials. We show that for meta-atoms of a certain size...... an analytical criterion for performing the homogenization and a tool to predict the homogenization limit. We show that strong coupling between meta-atoms of chiral metamaterials may prevent their homogenization at all....
Homogenization of resonant chiral metamaterials
Andryieuski, Andrei; Menzel, C.; Rockstuhl, Carsten
2010-01-01
Homogenization of metamaterials is a crucial issue as it allows to describe their optical response in terms of effective wave parameters as, e.g., propagation constants. In this paper we consider the possible homogenization of chiral metamaterials. We show that for meta-atoms of a certain size...... an analytical criterion for performing the homogenization and a tool to predict the homogenization limit. We show that strong coupling between meta-atoms of chiral metamaterials may prevent their homogenization at all....
Homogeneous Clifford structures
Moroianu, Andrei; Pilca, Mihaela
2012-01-01
We give an upper bound for the rank r of homogeneous (even) Clifford structures on compact manifolds of non-vanishing Euler characteristic. More precisely, we show that if r = 2a � b with b odd, then r � 9 for a = 0, r � 10 for a = 1, r � 12 for a = 2 and r � 16 for a � 3. Moreover, we describe the four limiting cases and show that there is exactly one solution in each case.
Figueroa-O'Farrill, José
2015-01-01
Motivated by the search for new gravity duals to M2 branes with $N>4$ supersymmetry --- equivalently, M-theory backgrounds with Killing superalgebra $\\mathfrak{osp}(N|4)$ for $N>4$ --- we classify (except for a small gap) homogeneous M-theory backgrounds with symmetry Lie algebra $\\mathfrak{so}(n) \\oplus \\mathfrak{so}(3,2)$ for $n=5,6,7$. We find that there are no new backgrounds with $n=6,7$ but we do find a number of new (to us) backgrounds with $n=5$. All backgrounds are metrically products of the form $\\operatorname{AdS}_4 \\times P^7$, with $P$ riemannian and homogeneous under the action of $\\operatorname{SO}(5)$, or $S^4 \\times Q^7$ with $Q$ lorentzian and homogeneous under the action of $\\operatorname{SO}(3,2)$. At least one of the new backgrounds is supersymmetric (albeit with only $N=2$) and we show that it can be constructed from a supersymmetric Freund--Rubin background via a Wick rotation. Two of the new backgrounds have only been approximated numerically.
Deng, Shaoqiang
2012-01-01
"Homogeneous Finsler Spaces" is the first book to emphasize the relationship between Lie groups and Finsler geometry, and the first to show the validity in using Lie theory for the study of Finsler geometry problems. This book contains a series of new results obtained by the author and collaborators during the last decade. The topic of Finsler geometry has developed rapidly in recent years. One of the main reasons for its surge in development is its use in many scientific fields, such as general relativity, mathematical biology, and phycology (study of algae). This monograph introduc
Bershadskii, A.G.
1985-06-01
An exact solution for the nonlinear problem of the spectral energy function of a homogeneous turbulence is derived under the assumption that energy transfer under the effect of inertial forces is determined mainly by the interactions among vortices whose wavenumbers are only slightly different from each other. The results are experimentally verified for turbulence behind grids. Similar problems are solved for MHD turbulence and for a nonstationary spectral energy function. It is shown that at the initial stage of degeneration, the spectral energy function is little influenced by the Stewart number; this agrees with experimental data for the damping of longitudinal velocity pulsations behind a grid in a longitudinal magnetic field. 15 references.
Symmetries of homogeneous cosmologies
Cotsakis, S; Pantazi, H; Cotsakis, Spiros; Leach, Peter; Pantazi, Hara
1998-01-01
We reformulate the dynamics of homogeneous cosmologies with a scalar field matter source with an arbitrary self-interaction potential in the language of jet bundles and extensions of vector fields. In this framework, the Bianchi-scalar field equations become subsets of the second Bianchi jet bundle, $J^2$, and every Bianchi cosmology is naturally extended to live on a variety of $J^2$. We are interested in the existence and behaviour of extensions of arbitrary Bianchi-Lie and variational vector fields acting on the Bianchi variety and accordingly we classify all such vector fields corresponding to both Bianchi classes $A$ and $B$. We give examples of functions defined on Bianchi jet bundles which are constant along some Bianchi models (first integrals) and use these to find particular solutions in the Bianchi total space. We discuss how our approach could be used to shed new light to questions like isotropization and the nature of singularities of homogeneous cosmologies by examining the behaviour of the vari...
Universum Inference and Corpus Homogeneity
Vogel, Carl; Lynch, Gerard; Janssen, Jerom
Universum Inference is re-interpreted for assessment of corpus homogeneity in computational stylometry. Recent stylometric research quantifies strength of characterization within dramatic works by assessing the homogeneity of corpora associated with dramatic personas. A methodological advance is suggested to mitigate the potential for the assessment of homogeneity to be achieved by chance. Baseline comparison analysis is constructed for contributions to debates by nonfictional participants: the corpus analyzed consists of transcripts of US Presidential and Vice-Presidential debates from the 2000 election cycle. The corpus is also analyzed in translation to Italian, Spanish and Portuguese. Adding randomized categories makes assessments of homogeneity more conservative.
Homogeneous crystal nucleation in polymers.
Schick, Christoph; Androsch, R; Schmelzer, Juern W P
2017-07-14
The pathway of crystal nucleation significantly influences the structure and properties of semi-crystalline polymers. Crystal nucleation is normally heterogeneous at low supercooling, and homogeneous at high supercooling, of the polymer melt. Homogeneous nucleation in bulk polymers has been, so far, hardly accessible experimentally, and was even doubted to occur at all. This topical review summarizes experimental findings on homogeneous crystal nucleation in polymers. Recently developed fast scanning calorimetry, with cooling and heating rates up to 106 K s-1, allows for detailed investigations of nucleation near and even below the glass transition temperature, including analysis of nuclei stability. As for other materials, the maximum homogeneous nucleation rate for polymers is located close to the glass transition temperature. In the experiments discussed here, it is shown that polymer nucleation is homogeneous at such temperatures. Homogeneous nucleation in polymers is discussed in the framework of classical nucleation theory. The majority of our observations are consistent with the theory. The discrepancies may guide further research, particularly experiments to progress theoretical development. Progress in the understanding of homogeneous nucleation is much needed, since most of the modelling approaches dealing with polymer crystallization exclusively consider homogeneous nucleation. This is also the basis for advancing theoretical approaches to the much more complex phenomena governing heterogeneous nucleation. © 2017 IOP Publishing Ltd.
Homogeneous Spaces and Equivariant Embeddings
Timashev, DA
2011-01-01
Homogeneous spaces of linear algebraic groups lie at the crossroads of algebraic geometry, theory of algebraic groups, classical projective and enumerative geometry, harmonic analysis, and representation theory. By standard reasons of algebraic geometry, in order to solve various problems on a homogeneous space it is natural and helpful to compactify it keeping track of the group action, i.e. to consider equivariant completions or, more generally, open embeddings of a given homogeneous space. Such equivariant embeddings are the subject of this book. We focus on classification of equivariant em
Reymond, Jean-Louis; Fluxà, Viviana S; Maillard, Noélie
2009-01-07
Enzyme assays are analytical tools to visualize enzyme activities. In recent years a large variety of enzyme assays have been developed to assist the discovery and optimization of industrial enzymes, in particular for "white biotechnology" where selective enzymes are used with great success for economically viable, mild and environmentally benign production processes. The present article highlights the aspects of fluorogenic and chromogenic substrates, sensors, and enzyme fingerprinting, which are our particular areas of interest.
Homogenization of Partial Differential Equations
Kaiser, Gerald
2005-01-01
A comprehensive study of homogenized problems, focusing on the construction of nonstandard models: non-local models, multicomponent models, and models with memory. This work is intended for graduate students, applied mathematicians, physicists, and engineers.
Operator estimates in homogenization theory
Zhikov, V. V.; Pastukhova, S. E.
2016-06-01
This paper gives a systematic treatment of two methods for obtaining operator estimates: the shift method and the spectral method. Though substantially different in mathematical technique and physical motivation, these methods produce basically the same results. Besides the classical formulation of the homogenization problem, other formulations of the problem are also considered: homogenization in perforated domains, the case of an unbounded diffusion matrix, non-self-adjoint evolution equations, and higher-order elliptic operators. Bibliography: 62 titles.
Genetic Homogenization of Composite Materials
P. Tobola
2009-04-01
Full Text Available The paper is focused on numerical studies of electromagnetic properties of composite materials used for the construction of small airplanes. Discussions concentrate on the genetic homogenization of composite layers and composite layers with a slot. The homogenization is aimed to reduce CPU-time demands of EMC computational models of electrically large airplanes. First, a methodology of creating a 3-dimensional numerical model of a composite material in CST Microwave Studio is proposed focusing on a sufficient accuracy of the model. Second, a proper implementation of a genetic optimization in Matlab is discussed. Third, an association of the optimization script and a simplified 2-dimensional model of the homogeneous equivalent model in Comsol Multiphysics is proposed considering EMC issues. Results of computations are experimentally verified.
Homogeneity and plane-wave limits
Figueroa-O'Farrill, J M; Philip, S; Farrill, Jos\\'e Figueroa-O'; Meessen, Patrick; Philip, Simon
2005-01-01
We explore the plane-wave limit of homogeneous spacetimes. For plane-wave limits along homogeneous geodesics the limit is known to be homogeneous and we exhibit the limiting metric in terms of Lie algebraic data. This simplifies many calculations and we illustrate this with several examples. We also investigate the behaviour of (reductive) homogeneous structures under the plane-wave limit.
Homogenization method for elastic materials
Seifrt F.
2007-11-01
Full Text Available In the paper we study the homogenization method and its potential for research of some phenomenons connected with periodic elastic materials. This method will be applied on partial differential equations that describe the deformation of a periodic composite material. The next part of the paper will deal with applications of the homogenization method. The importance of the method will be discussed more detailed for the exploration of the so called bandgaps. Bandgap is a phenomenon which may appear during vibrations of some periodically heterogeneous materials. This phenomenon is not only observable during vibrations for the aforementioned materials, but we may also observe similar effects by propagation of electromagnetic waves of heterogeneous dielectric medias.
Giant dielectric anisotropy via homogenization
Mackay, Tom G
2014-01-01
A random mixture of two isotropic dielectric materials, one composed of oriented spheroidal particles of relative permittivity $\\epsilon_a$ and the other composed of oriented spheroidal particles of relative permittivity $\\epsilon_b$, was considered in the long wavelength regime. The permittivity dyadic of the resulting homogenized composite material (HCM) was estimated using the Bruggeman homogenization formalism. The HCM was an orthorhombic biaxial material if the symmetry axes of the two populations of spheroids were mutually perpendicular and a uniaxial material if these two axes were mutually aligned. The degree of anisotropy of the HCM, as gauged by the ratio of the eigenvalues of the HCM's permittivity dyadic, increased as the shape of the constituent particles became more eccentric. The greatest degrees of HCM anisotropy were achieved for the limiting cases wherein the constituent particles were shaped as needles or discs. In these instances explicit formulas for the HCM anisotropy were derived from t...
Nambiar, Dhanya K; Kujur, Praveen K; Singh, Rana P
2016-01-01
Neoangiogenesis constitutes one of the first steps of tumor progression beyond a critical size of tumor growth, which supplies a dormant mass of cancerous cells with the required nutrient supply and gaseous exchange through blood vessels essentially needed for their sustained and aggressive growth. In order to understand any biological process, it becomes imperative that we use models, which could mimic the actual biological system as closely as possible. Hence, finding the most appropriate model is always a vital part of any experimental design. Angiogenesis research has also been much affected due to lack of simple, reliable, and relevant models which could be easily quantitated. The angiogenesis models have been used extensively for studying the action of various molecules for agonist or antagonistic behaviour and associated mechanisms. Here, we have described two protocols or models which have been popularly utilized for studying angiogenic parameters. Rat aortic ring assay tends to bridge the gap between in vitro and in vivo models. The chorioallantoic membrane (CAM) assay is one of the most utilized in vivo model system for angiogenesis-related studies. The CAM is highly vascularized tissue of the avian embryo and serves as a good model to study the effects of various test compounds on neoangiogenesis.
Heterotic strings on homogeneous spaces
Israel, D; Orlando, D; Petropoulos, P M; Israel, Dan; Kounnas, Costas; Orlando, Domenico
2004-01-01
We construct heterotic string backgrounds corresponding to families of homogeneous spaces as exact conformal field theories. They contain left cosets of compact groups by their maximal tori supported by NS-NS 2-forms and gauge field fluxes. We give the general formalism and modular-invariant partition functions, then we consider some examples such as SU(2)/U(1) ~ S^2 (already described in a previous paper) and the SU(3)/U(1)^2 flag space. As an application we construct new supersymmetric string vacua with magnetic fluxes and a linear dilaton.
Homogeneous orbit closures and applications
Lindenstrauss, Elon
2011-01-01
We give new classes of examples of orbits of the diagonal group in the space of unit volume lattices in R^d for d > 2 with nice (homogeneous) orbit closures, as well as examples of orbits with explicitly computable but irregular orbit closures. We give Diophantine applications to the former, for instance we show that if x is the cubic root of 2 then for any y,z in R liminf |n|=0 (as |n| goes to infinity), where denotes the distance of a real number c to the integers.
Homogenization scheme for acoustic metamaterials
Yang, Min
2014-02-26
We present a homogenization scheme for acoustic metamaterials that is based on reproducing the lowest orders of scattering amplitudes from a finite volume of metamaterials. This approach is noted to differ significantly from that of coherent potential approximation, which is based on adjusting the effective-medium parameters to minimize scatterings in the long-wavelength limit. With the aid of metamaterials’ eigenstates, the effective parameters, such as mass density and elastic modulus can be obtained by matching the surface responses of a metamaterial\\'s structural unit cell with a piece of homogenized material. From the Green\\'s theorem applied to the exterior domain problem, matching the surface responses is noted to be the same as reproducing the scattering amplitudes. We verify our scheme by applying it to three different examples: a layered lattice, a two-dimensional hexagonal lattice, and a decorated-membrane system. It is shown that the predicted characteristics and wave fields agree almost exactly with numerical simulations and experiments and the scheme\\'s validity is constrained by the number of dominant surface multipoles instead of the usual long-wavelength assumption. In particular, the validity extends to the full band in one dimension and to regimes near the boundaries of the Brillouin zone in two dimensions.
ISOTOPE METHODS IN HOMOGENEOUS CATALYSIS.
BULLOCK,R.M.; BENDER,B.R.
2000-12-01
The use of isotope labels has had a fundamentally important role in the determination of mechanisms of homogeneously catalyzed reactions. Mechanistic data is valuable since it can assist in the design and rational improvement of homogeneous catalysts. There are several ways to use isotopes in mechanistic chemistry. Isotopes can be introduced into controlled experiments and followed where they go or don't go; in this way, Libby, Calvin, Taube and others used isotopes to elucidate mechanistic pathways for very different, yet important chemistries. Another important isotope method is the study of kinetic isotope effects (KIEs) and equilibrium isotope effect (EIEs). Here the mere observation of where a label winds up is no longer enough - what matters is how much slower (or faster) a labeled molecule reacts than the unlabeled material. The most careti studies essentially involve the measurement of isotope fractionation between a reference ground state and the transition state. Thus kinetic isotope effects provide unique data unavailable from other methods, since information about the transition state of a reaction is obtained. Because getting an experimental glimpse of transition states is really tantamount to understanding catalysis, kinetic isotope effects are very powerful.
Homogenization of a nonlinear degenerate parabolic equation
无
2005-01-01
The homogenization of one kind of nonlinear parabolic equation is studied. The weak convergence and corrector results are obtained by combining carefully the compactness method and two-scale convergence method in the homogenization theory.
The Calkin algebra is not countably homogeneous
Farah, Ilijas; Hirshberg, Ilan
2015-01-01
We show that the Calkin algebra is not countably homogeneous, in the sense of continuous model theory. We furthermore show that the connected component of the unitary group of the Calkin algebra is not countably homogeneous.
Coherence delay augmented laser beam homogenizer
Rasmussen, P.; Bernhardt, A.
1993-06-29
The geometrical restrictions on a laser beam homogenizer are relaxed by ug a coherence delay line to separate a coherent input beam into several components each having a path length difference equal to a multiple of the coherence length with respect to the other components. The components recombine incoherently at the output of the homogenizer, and the resultant beam has a more uniform spatial intensity suitable for microlithography and laser pantogography. Also disclosed is a variable aperture homogenizer, and a liquid filled homogenizer.
Orthogonality Measurement for Homogenous Projects-Bases
Ivan, Ion; Sandu, Andrei; Popa, Marius
2009-01-01
The homogenous projects-base concept is defined. Next, the necessary steps to create a homogenous projects-base are presented. A metric system is built, which then will be used for analyzing projects. The indicators which are meaningful for analyzing a homogenous projects-base are selected. The given hypothesis is experimentally verified. The…
Improving homogeneity by dynamic speed limit systems.
Nes, N. van Brandenberg, S. & Twisk, D.A.M.
2010-01-01
Homogeneity of driving speeds is an important variable in determining road safety; more homogeneous driving speeds increase road safety. This study investigates the effect of introducing dynamic speed limit systems on homogeneity of driving speeds. A total of 46 subjects twice drove a route along 12
Homogeneous models for bianisotropic crystals
Ponti, S; Oldano, C
2002-01-01
We extend to bianisotropic structures a formalism already developed, based on the Bloch method for defining the effective dielectric tensor of anisotropic crystals in the long-wavelength approximation. More precisely, we provide a homogenization scheme which yields a wavevector-dependent effective medium for any 3D, 2D, or 1D bianisotropic crystal. We illustrate our procedure by applying this to a 1D magneto-electric smectic C*-type structure. The resulting equations confirm that the presence of dielectric and magnetic susceptibilities in the periodic structures generates magneto-electric pseudo-tensors for the effective medium. Their contribution to the optical activity of structurally chiral media can be of the same order of magnitude as the one present in dielectric helix-shaped crystals. Simple analytical expressions are found for the most important optical properties of smectic C*-type structures which are simultaneously dielectric and magnetic.
Effective Gravity and Homogenous Solutions
Müller, Daniel
2013-01-01
Near the singularity, gravity should be modified to an effective theory, in the same sense as with the Euler-Heisenberg electrodynamics. This effective gravity surmounts to higher derivative theory, and as is well known, a much more reacher theory concerning the solution space. On the other hand, as a highly non linear theory, the understanding of this solution space must go beyond the linearized approach. In this talk we will present some results previously published by collaborators and myself, concerning solutions for vacuum spatially homogenous cases of Bianchi types $I$ and $VII_A$. These are the anisotropic generalizations of the cosmological spatially "flat", and "open" models respectively. The solutions present isotropisation in a weak sense depending on the initial condition. Also, depending on the initial condition, singular solutions are obtained.
Effective Gravity and Homogenous Solutions
Müller, Daniel
2015-01-01
Near the singularity, gravity should be modified to an effective theory, in the same sense as with the Euler-Heisenberg electrodynamics. This effective gravity surmounts to higher derivative theory, and as is well known, a much more reacher theory concerning the solution space. On the other hand, as a highly non linear theory, the understanding of this solution space must go beyond the linearized approach. In this talk we will present some results previously published by collaborators and myself, concerning solutions for vacuum spatially homogenous cases of Bianchi types I and VIIA. These are the anisotropic generalizations of the cosmological spatially "flat", and "open" models respectively. The solutions present isotropisation in a weak sense depending on the initial condition. Also, depending on the initial condition, singular solutions are obtained.
Projective duality and homogeneous spaces
Tevelev, E A
2006-01-01
Projective duality is a very classical notion naturally arising in various areas of mathematics, such as algebraic and differential geometry, combinatorics, topology, analytical mechanics, and invariant theory, and the results in this field were until now scattered across the literature. Thus the appearance of a book specifically devoted to projective duality is a long-awaited and welcome event. Projective Duality and Homogeneous Spaces covers a vast and diverse range of topics in the field of dual varieties, ranging from differential geometry to Mori theory and from topology to the theory of algebras. It gives a very readable and thorough account and the presentation of the material is clear and convincing. For the most part of the book the only prerequisites are basic algebra and algebraic geometry. This book will be of great interest to graduate and postgraduate students as well as professional mathematicians working in algebra, geometry and analysis.
Antioxidant effects of calcium antagonists in rat brain homogenates.
Yao, K; Ina, Y; Nagashima, K; Ohmori, K; Ohno, T
2000-06-01
We studied the antioxidant activities of calcium antagonists against autoxidation in rat brain homogenates. The homogenates were incubated for 30 min at 37 degrees C with or without a calcium antagonist and subsequently assayed for lipid peroxide content. Percent inhibition of the lipid peroxidation was used as an index of the antioxidant effect. Dihydropyridine calcium antagonists exhibited concentration-dependent (3-300 micromol/l) inhibitory effects against lipid peroxidation. The relative order of antioxidant potency and associated IC50 values (micromol/l) of the calcium antagonists for inhibition of the lipid peroxidation were as follows: nifedipine (51.5)>barnidipine (58.6)>benidipine (71.2)>nicardipine (129.3)>amlodipine (135.5)>nilvadipine (167.3)>nitrendipine (252.1)> diltiazem (>300)=verapamil (>300). These results suggest that some dihydropyridine calcium antagonists show antioxidant properties. The antioxidant effects of the calcium antagonists may contribute to their pharmacological actions.
Protein binding assay for hyaluronate
Lacy, B.E.; Underhill, C.B.
1986-11-01
A relatively quick and simple assay for hyaluronate was developed using the specific binding protein, hyaluronectin. The hyaluronectin was obtained by homogenizing the brains of Sprague-Dawley rats, and then centrifuging the homogenate. The resulting supernatant was used as a source of crude hyaluronectin. In the binding assay, the hyaluronectin was mixed with (/sup 3/H)hyaluronate, followed by an equal volume of saturated (NH/sub 4/)/sub 2/SO/sub 4/, which precipitated the hyaluronectin and any (/sup 3/H)hyaluronate associated with it, but left free (/sup 3/H)hyaluronate in solution. The mixture was then centrifuged, and the amount of bound (/sup 3/H)hyaluronate in the precipitate was determined. Using this assay, the authors found that hyaluronectin specifically bound hyaluronate, since other glycosaminoglycans failed to compete for the binding protein. In addition, the interaction between hyaluronectin and hyaluronate was of relatively high affinity, and the size of the hyaluronate did not appear to substantially alter the amount of binding. To determine the amount of hyaluronate in an unknown sample, they used a competition assay in which the binding of a set amount of (/sup 3/H)hyaluronate was blocked by the addition of unlabeled hyaluronate. By comparing the degree of competition of the unknown samples with that of known amounts of hyaluronate, it was possible to determine the amount of hyaluronate in the unknowns. They have found that this method is sensitive to 1 ..mu..g or less of hyaluronate, and is unaffected by the presence of proteins.
Reciprocity theory of homogeneous reactions
Agbormbai, Adolf A.
1990-03-01
The reciprocity formalism is applied to the homogeneous gaseous reactions in which the structure of the participating molecules changes upon collision with one another, resulting in a change in the composition of the gas. The approach is applied to various classes of dissociation, recombination, rearrangement, ionizing, and photochemical reactions. It is shown that for the principle of reciprocity to be satisfied it is necessary that all chemical reactions exist in complementary pairs which consist of the forward and backward reactions. The backward reaction may be described by either the reverse or inverse process. The forward and backward processes must satisfy the same reciprocity equation. Because the number of dynamical variables is usually unbalanced on both sides of a chemical equation, it is necessary that this balance be established by including as many of the dynamical variables as needed before the reciprocity equation can be formulated. Statistical transformation models of the reactions are formulated. The models are classified under the titles free exchange, restricted exchange and simplified restricted exchange. The special equations for the forward and backward processes are obtained. The models are consistent with the H theorem and Le Chatelier's principle. The models are also formulated in the context of the direct simulation Monte Carlo method.
Pharmaceutical Industry Oriented Homogeneous Catalysis
Zhang Xumu
2004-01-01
Chiral therapeutics already makes up over one-third of pharmaceutical drugs currently sold worldwide. This is a growing industry with global chiral drug sales for 2002 increasing by 12%to $160 billion (Technology Catalysts International) of a total drug market of $410bn. The increasing demand to produce enantiomerically pure pharmaceuticals, agrochemicals, flavors, and other fine chemicals has advanced the field of asymmetric catalytic technologies.We aim to become a high value technology provider and partner in the chiral therapeutics industry by offering proprietary catalysts, novel building blocks, and collaborative synthetic solutions. In decade, we have developed a set of novel chiral homogeneous phosphorus ligands such as Binaphane, Me-KetalPhos, TangPhos, f-Binaphane, Me-f-KetalPhos, C4TunePhos and Binapine,which we called Chiral Ligand ToolKit. Complementing the ToolKit, (R, S, S, R)-DIOP*, T-Phos,o-BIPHEP, o-BINAPO and FAP were added recently[1].These ligands can be applied to a broad variety of drug structural features by asymmetric hydrogenation of dehydroamino acid derivatives, enamides, unsatisfied acids and esters, ketones,beta ketoesters, imines and cyclic imines. And ligand FAP had been apllied succefully in allylic alkylation and [3+2] cycloaddition.
A Class of Homogeneous Einstein Manifolds
Yifang KANG; Ke LIANG
2006-01-01
A Riemannian manifold (M,g) is called Einstein manifold if its Ricci tensor satisfies r=c·g for some constant c. General existence results are hard to obtain,e.g., it is as yet unknown whether every compact manifold admits an Einstein metric. A natural approach is to impose additional homogeneous assumptions. M. Y. Wang and W. Ziller have got some results on compact homogeneous space G/H. They investigate standard homogeneous metrics, the metric induced by Killing form on G/H, and get some classification results. In this paper some more general homogeneous metrics on some homogeneous space G/H are studies, and a necessary and sufficient condition for this metric to be Einstein is given. The authors also give some examples of Einstein manifolds with non-standard homogeneous metrics.
AQUEOUS HOMOGENEOUS REACTORTECHNICAL PANEL REPORT
Diamond, D.J.; Bajorek, S.; Bakel, A.; Flanagan, G.; Mubayi, V.; Skarda, R.; Staudenmeier, J.; Taiwo, T.; Tonoike, K.; Tripp, C.; Wei, T.; Yarsky, P.
2010-12-03
Considerable interest has been expressed for developing a stable U.S. production capacity for medical isotopes and particularly for molybdenum- 99 (99Mo). This is motivated by recent re-ductions in production and supply worldwide. Consistent with U.S. nonproliferation objectives, any new production capability should not use highly enriched uranium fuel or targets. Conse-quently, Aqueous Homogeneous Reactors (AHRs) are under consideration for potential 99Mo production using low-enriched uranium. Although the Nuclear Regulatory Commission (NRC) has guidance to facilitate the licensing process for non-power reactors, that guidance is focused on reactors with fixed, solid fuel and hence, not applicable to an AHR. A panel was convened to study the technical issues associated with normal operation and potential transients and accidents of an AHR that might be designed for isotope production. The panel has produced the requisite AHR licensing guidance for three chapters that exist now for non-power reactor licensing: Reac-tor Description, Reactor Coolant Systems, and Accident Analysis. The guidance is in two parts for each chapter: 1) standard format and content a licensee would use and 2) the standard review plan the NRC staff would use. This guidance takes into account the unique features of an AHR such as the fuel being in solution; the fission product barriers being the vessel and attached systems; the production and release of radiolytic and fission product gases and their impact on operations and their control by a gas management system; and the movement of fuel into and out of the reactor vessel.
Rapid homogeneous immunoassay for cardiac troponin I using switchable lanthanide luminescence.
Päkkilä, Henna; Malmi, Eeva; Lahtinen, Satu; Soukka, Tero
2014-12-15
Homogeneous assays are advantageous because of their simplicity and rapid kinetics but typically their performance is severely compromised compared to heterogeneous assay formats. Here, we report a homogeneous immunoassay utilizing switchable lanthanide luminescence for detection and site-specifically labeled recombinant antibody fragments as binders to improve the assay performance. Switchable lanthanide luminescence enabled elimination of assay background due to division of the luminescent lanthanide chelate into two non-luminescent label moieties. Simultaneous biomolecular recognition of model analyte cardiac troponin I by two antibody fragments brought the label moieties together and resulted in self-assembly of luminescent mixed chelate complex. The assay was very rapid as maximal signal-to-background ratios were achieved already after 6 min of incubation. Additionally, the limit of detection was 0.38 ng/mL (16 pM), which was comparable to the limit of detection for the heterogeneous reference assay based on the same binders (0.26 ng/mL or 11 pM). This is the first study to apply switchable lanthanide luminescence in immunoassays and demonstrates the versatile potential of the technology for rapid and sensitive homogeneous assays.
Homogeneity and thermodynamic identities in geometrothermodynamics
Quevedo, Hernando; Quevedo, María N.; Sánchez, Alberto
2017-03-01
We propose a classification of thermodynamic systems in terms of the homogeneity properties of their fundamental equations. Ordinary systems correspond to homogeneous functions and non-ordinary systems are given by generalized homogeneous functions. This affects the explicit form of the Gibbs-Duhem relation and Euler's identity. We show that these generalized relations can be implemented in the formalism of black hole geometrothermodynamics in order to completely fix the arbitrariness present in Legendre invariant metrics.
Homogeneity and thermodynamic identities in geometrothermodynamics
Quevedo, Hernando [Universidad Nacional Autonoma de Mexico, Instituto de Ciencias Nucleares (Mexico); Universita di Roma ' ' La Sapienza' ' , Dipartimento di Fisica, Rome (Italy); ICRANet, Rome (Italy); Quevedo, Maria N. [Universidad Militar Nueva Granada, Departamento de Matematicas, Facultad de Ciencias Basicas, Bogota (Colombia); Sanchez, Alberto [CIIDET, Departamento de Posgrado, Queretaro (Mexico)
2017-03-15
We propose a classification of thermodynamic systems in terms of the homogeneity properties of their fundamental equations. Ordinary systems correspond to homogeneous functions and non-ordinary systems are given by generalized homogeneous functions. This affects the explicit form of the Gibbs-Duhem relation and Euler's identity. We show that these generalized relations can be implemented in the formalism of black hole geometrothermodynamics in order to completely fix the arbitrariness present in Legendre invariant metrics. (orig.)
The Homogeneity Scale of the universe
Ntelis, Pierros
2016-01-01
In this study, we probe the cosmic homogeneity with the BOSS CMASS galaxy sample in the redshift region of $0.43 < z < 0.7$. We use the normalised counts-in-spheres estimator $\\mathcal{N}(
Self-consolidating concrete homogeneity
Jarque, J. C.
2007-08-01
Full Text Available Concrete instability may lead to the non-uniform distribution of its properties. The homogeneity of self-consolidating concrete in vertically cast members was therefore explored in this study, analyzing both resistance to segregation and pore structure uniformity. To this end, two series of concretes were prepared, self-consolidating and traditional vibrated materials, with different w/c ratios and types of cement. The results showed that selfconsolidating concretes exhibit high resistance to segregation, albeit slightly lower than found in the traditional mixtures. The pore structure in the former, however, tended to be slightly more uniform, probably as a result of less intense bleeding. Such concretes are also characterized by greater bulk density, lower porosity and smaller mean pore size, which translates into a higher resistance to pressurized water. For pore diameters of over about 0.5 Î¼m, however, the pore size distribution was found to be similar to the distribution in traditional concretes, with similar absorption rates.En este trabajo se estudia la homogeneidad de los hormigones autocompactantes en piezas hormigonadas verticalmente, determinando su resistencia a la segregación y la uniformidad de su estructura porosa, dado que la pérdida de estabilidad de una mezcla puede conducir a una distribución no uniforme de sus propiedades. Para ello se han fabricado dos tipos de hormigones, uno autocompactante y otro tradicional vibrado, con diferentes relaciones a/c y distintos tipos de cemento. Los resultados ponen de manifiesto que los hormigones autocompactantes presentan una buena resistencia a la segregación, aunque algo menor que la registrada en los hormigones tradicionales. A pesar de ello, su estructura porosa tiende a ser ligeramente más uniforme, debido probablemente a un menor sangrado. Asimismo, presentan una mayor densidad aparente, una menor porosidad y un menor tamaño medio de poro, lo que les confiere mejores
CLASSIFICATION OF CUBIC PARAMETERIZED HOMOGENEOUS VECTOR FIELDS
Karnal H.Yasir; TANG Yun
2002-01-01
In this paper the cubic homogeneous parameterized vector fields are studied.The classification of the phase portrait near the critical point is presented. This classification is an extension of the result given by Takens to the cubic homogeneous parameterized vector fields with six parameters.
CLASSIFICATION OF CUBIC PARAMETERIZED HOMOGENEOUS VECTOR FIELDS
KamalH.Yasir; TNAGYun
2002-01-01
In this paper the cubic homogeneous parameterized vector fields are studied.The classification of the phase portrait near the critical point is presented.This classification is an extension of the result given by takens to the cubic homogeneous parameterized vector fields with six parameters.
Investigations into homogenization of electromagnetic metamaterials
Clausen, Niels Christian Jerichau
This dissertation encompasses homogenization methods, with a special interest into their applications to metamaterial homogenization. The first method studied is the Floquet-Bloch method, that is based on the assumption of a material being infinite periodic. Its field can then be expanded in term...
The Case Against Homogeneous Sets in Mathematics
Jackman, M. K.
1973-01-01
A point-by-point criticism is made of F. H. Flynn's article, The Case for Homogeneous Sets in Mathematics'' (Mathematics in School, Volume 1 Number 2, 1972) in an attempt to show that the arguments used in trying to justify homogeneous grouping in mathematics are invalid. (Editor/DT)
The homogeneous geometries of real hyperbolic space
Castrillón López, Marco; Gadea, Pedro Martínez; Swann, Andrew Francis
We describe the holonomy algebras of all canonical connections of homogeneous structures on real hyperbolic spaces in all dimensions. The structural results obtained then lead to a determination of the types, in the sense of Tricerri and Vanhecke, of the corresponding homogeneous tensors. We use ...
DETERMINISTIC HOMOGENIZATION OF QUASILINEAR DAMPED HYPERBOLIC EQUATIONS
Gabriel Nguetseng; Hubert Nnang; Nils Svanstedt
2011-01-01
Deterministic homogenization is studied for quasilinear monotone hyperbolic problems with a linear damping term.It is shown by the sigma-convergence method that the sequence of solutions to a class of multi-scale highly oscillatory hyperbolic problems converges to the solution to a homogenized quasilinear hyperbolic problem.
Finalization report: homogeneous PVM/PARIX
B.J. Overeinder; P.M.A. Sloot; J. Petersen
1994-01-01
This document reports on the design and implementation considerations of PVM/PARIX, homogeneous version 1.0. This version is for use with PARIX 1.2 only. Further, it contains information how to use Homogeneous PVM/PARIX and the appendix contains the installation notes.
Homogeneity of Prototypical Attributes in Soccer Teams
Christian Zepp
2015-09-01
Full Text Available Research indicates that the homogeneous perception of prototypical attributes influences several intragroup processes. The aim of the present study was to describe the homogeneous perception of the prototype and to identify specific prototypical subcategories, which are perceived as homogeneous within sport teams. The sample consists of N = 20 soccer teams with a total of N = 278 athletes (age M = 23.5 years, SD = 5.0 years. The results reveal that subcategories describing the cohesiveness of the team and motivational attributes are mentioned homogeneously within sport teams. In addition, gender, identification, team size, and the championship ranking significantly correlate with the homogeneous perception of prototypical attributes. The results are discussed on the basis of theoretical and practical implications.
Multilevel Monte Carlo Approaches for Numerical Homogenization
Efendiev, Yalchin R.
2015-10-01
In this article, we study the application of multilevel Monte Carlo (MLMC) approaches to numerical random homogenization. Our objective is to compute the expectation of some functionals of the homogenized coefficients, or of the homogenized solutions. This is accomplished within MLMC by considering different sizes of representative volumes (RVEs). Many inexpensive computations with the smallest RVE size are combined with fewer expensive computations performed on larger RVEs. Likewise, when it comes to homogenized solutions, different levels of coarse-grid meshes are used to solve the homogenized equation. We show that, by carefully selecting the number of realizations at each level, we can achieve a speed-up in the computations in comparison to a standard Monte Carlo method. Numerical results are presented for both one-dimensional and two-dimensional test-cases that illustrate the efficiency of the approach.
Deforestation homogenizes tropical parasitoid-host networks.
Laliberté, Etienne; Tylianakis, Jason M
2010-06-01
Human activities drive biotic homogenization (loss of regional diversity) of many taxa. However, whether species interaction networks (e.g., food webs) can also become homogenized remains largely unexplored. Using 48 quantitative parasitoid-host networks replicated through space and time across five tropical habitats, we show that deforestation greatly homogenized network structure at a regional level, such that interaction composition became more similar across rice and pasture sites compared with forested habitats. This was not simply caused by altered consumer and resource community composition, but was associated with altered consumer foraging success, such that parasitoids were more likely to locate their hosts in deforested habitats. Furthermore, deforestation indirectly homogenized networks in time through altered mean consumer and prey body size, which decreased in deforested habitats. Similar patterns were obtained with binary networks, suggesting that interaction (link) presence-absence data may be sufficient to detect network homogenization effects. Our results show that tropical agroforestry systems can support regionally diverse parasitoid-host networks, but that removal of canopy cover greatly homogenizes the structure of these networks in space, and to a lesser degree in time. Spatiotemporal homogenization of interaction networks may alter coevolutionary outcomes and reduce ecological resilience at regional scales, but may not necessarily be predictable from community changes observed within individual trophic levels.
String pair production in non homogeneous backgrounds
Bolognesi, S. [Department of Physics “E. Fermi” University of Pisa, and INFN - Sezione di Pisa,Largo Pontecorvo, 3, Ed. C, 56127 Pisa (Italy); Rabinovici, E. [Racah Institute of Physics, The Hebrew University of Jerusalem,91904 Jerusalem (Israel); Tallarita, G. [Departamento de Ciencias, Facultad de Artes Liberales,Universidad Adolfo Ibáñez, Santiago 7941169 (Chile)
2016-04-28
We consider string pair production in non homogeneous electric backgrounds. We study several particular configurations which can be addressed with the Euclidean world-sheet instanton technique, the analogue of the world-line instanton for particles. In the first case the string is suspended between two D-branes in flat space-time, in the second case the string lives in AdS and terminates on one D-brane (this realizes the holographic Schwinger effect). In some regions of parameter space the result is well approximated by the known analytical formulas, either the particle pair production in non-homogeneous background or the string pair production in homogeneous background. In other cases we see effects which are intrinsically stringy and related to the non-homogeneity of the background. The pair production is enhanced already for particles in time dependent electric field backgrounds. The string nature enhances this even further. For spacial varying electrical background fields the string pair production is less suppressed than the rate of particle pair production. We discuss in some detail how the critical field is affected by the non-homogeneity, for both time and space dependent electric field backgrouds. We also comment on what could be an interesting new prediction for the small field limit. The third case we consider is pair production in holographic confining backgrounds with homogeneous and non-homogeneous fields.
Benchmarking homogenization algorithms for monthly data
V. K. C. Venema
2012-01-01
Full Text Available The COST (European Cooperation in Science and Technology Action ES0601: advances in homogenization methods of climate series: an integrated approach (HOME has executed a blind intercomparison and validation study for monthly homogenization algorithms. Time series of monthly temperature and precipitation were evaluated because of their importance for climate studies and because they represent two important types of statistics (additive and multiplicative. The algorithms were validated against a realistic benchmark dataset. The benchmark contains real inhomogeneous data as well as simulated data with inserted inhomogeneities. Random independent break-type inhomogeneities with normally distributed breakpoint sizes were added to the simulated datasets. To approximate real world conditions, breaks were introduced that occur simultaneously in multiple station series within a simulated network of station data. The simulated time series also contained outliers, missing data periods and local station trends. Further, a stochastic nonlinear global (network-wide trend was added.
Participants provided 25 separate homogenized contributions as part of the blind study. After the deadline at which details of the imposed inhomogeneities were revealed, 22 additional solutions were submitted. These homogenized datasets were assessed by a number of performance metrics including (i the centered root mean square error relative to the true homogeneous value at various averaging scales, (ii the error in linear trend estimates and (iii traditional contingency skill scores. The metrics were computed both using the individual station series as well as the network average regional series. The performance of the contributions depends significantly on the error metric considered. Contingency scores by themselves are not very informative. Although relative homogenization algorithms typically improve the homogeneity of temperature data, only the best ones improve
Higher Order Macro Coefficients in Periodic Homogenization
Conca, Carlos; San Martin, Jorge [Departamento de IngenierIa Matematica, Facultad de Ciencias Fisicas y Matematicas, Universidad de Chile and Centro de Modelamiento Matematico, UMR 2071 CNRS-UChile, Casilla 170/3 - Correo 3, Santiago (Chile); Smaranda, Loredana [Department of Mathematics, Faculty of Mathematics and Computer Science, University of Pitesti, 110040 Pitesti, Str. Targu din Vale Nr.1, Arges (Romania); Vanninathan, Muthusamy, E-mail: cconca@dim.uchile.cl, E-mail: jorge@dim.uchile.cl, E-mail: smaranda@dim.uchile.cl, E-mail: vanni@math.tifrbng.res.in [TIFR-CAM, Post Bag 6503, GKVK Post, Bangalore - 560065 (India)
2011-09-15
A first set of macro coefficients known as the homogenized coefficients appear in the homogenization of PDE on periodic structures. If energy is increased or scale is decreased, these coefficients do not provide adequate approximation. Using Bloch decomposition, it is first realized that the above coefficients correspond to the lowest energy and the largest scale. This naturally paves the way to introduce other sets of macro coefficients corresponding to higher energies and lower scales which yield better approximation. The next task is to compare their properties with those of the homogenized coefficients. This article reviews these developments along with some new results yet to be published.
Discovery of sphingosine 1-O-methyltransferase in rat kidney and liver homogenates
Santosh J SACKET; Dong-soon IM
2008-01-01
Aim:To characterize sphingosine methyltransferase in rat tissues.Methods:By using S-adenosyl-L-(methyl-3H) methionine,enzymatic activity was measured in the rat liver and kidney homogenates.Results:The optimum pH and reaction time for the enzyme assay were pH 7.8 and 1 h.ZnCl2 inhibited the activity,but not MgCl2,CaCl2,CoCl2,or NiCl2.In the kidney homogenate,enzymatic activity was detectable in the cytosol and all membrane fractions from the plasma membrane and other organelles; however,in the liver homogenate,enzymatic activity was detectable in all membrane fractions,but not in the cytosol.We also tested the enzymatic activity with structurally-modified sphingosine derivatives.Conclusion:We found sphingosine l-O-methyltransferase activity in the rat liver and kidney homogenates.
On the Cancellation Rule in the Homogenization
2008-01-01
We consider the possible ways of the homogenization of non-graded non-commutative algebra and show that it should be combined with the cancellation rule to get the mathematically adequate correspondence between graded and non-graded algebras.
Non-homogeneous fractal hierarchical weighted networks.
Dong, Yujuan; Dai, Meifeng; Ye, Dandan
2015-01-01
A model of fractal hierarchical structures that share the property of non-homogeneous weighted networks is introduced. These networks can be completely and analytically characterized in terms of the involved parameters, i.e., the size of the original graph Nk and the non-homogeneous weight scaling factors r1, r2, · · · rM. We also study the average weighted shortest path (AWSP), the average degree and the average node strength, taking place on the non-homogeneous hierarchical weighted networks. Moreover the AWSP is scrupulously calculated. We show that the AWSP depends on the number of copies and the sum of all non-homogeneous weight scaling factors in the infinite network order limit.
Magnifying absolute instruments for optically homogeneous regions
Tyc, Tomas
2011-01-01
We propose a class of magnifying absolute optical instruments with a positive isotropic refractive index. They create magnified stigmatic images, either virtual or real, of optically homogeneous three-dimensional spatial regions within geometrical optics.
Homogeneous Dielectric Equivalents of Composite Material Shields
P. Tobola
2009-04-01
Full Text Available The paper deals with the methodology of replacing complicated parts of an airplane skin by simple homogeneous equivalents, which can exhibit similar shielding efficiency. On one hand, the airplane built from the virtual homogeneous equivalents can be analyzed with significantly reduced CPU-time demands and memory requirements. On the other hand, the equivalent model can estimate the internal fields satisfactory enough to evaluate the electromagnetic immunity of the airplane.
Commensurability effects in holographic homogeneous lattices
Andrade, Tomas; Krikun, Alexander
2016-01-01
An interesting application of the gauge/gravity duality to condensed matter physics is the description of a lattice via breaking translational invariance on the gravity side. By making use of global symmetries, it is possible to do so without scarifying homogeneity of the pertinent bulk solutions, which we thus term as "homogeneous holographic lattices." Due to their technical simplicity, these configurations have received a great deal of attention in the last few years and have been shown to...
Homogeneous cosmological models and new inflation
Turner, Michael S.; Widrow, Lawrence M.
1986-01-01
The promise of the inflationary-universe scenario is to free the present state of the universe from extreme dependence upon initial data. Paradoxically, inflation is usually analyzed in the context of the homogeneous and isotropic Robertson-Walker cosmological models. It is shown that all but a small subset of the homogeneous models undergo inflation. Any initial anisotropy is so strongly damped that if sufficient inflation occurs to solve the flatness and horizon problems, the universe today would still be very isotropic.
Significance tests and sample homogeneity loophole
Kupczynski, Marian
2015-01-01
In their recent comment, published in Nature, Jeffrey T.Leek and Roger D.Peng discuss how P-values are widely abused in null hypothesis significance testing . We agree completely with them and in this short comment we discuss the importance of sample homogeneity tests. No matter with how much scrutiny data are gathered if homogeneity tests are not performed the significance tests suffer from sample homogeneity loophole and the results may not be trusted. For example sample homogeneity loophole was not closed in the experiment testing local realism in which a significant violation of Eberhard inequality was found. We are not surprised that Bell type inequalities are violated since if the contextual character of quantum observables is properly taken into account these inequalities cannot be proven. However in order to trust the significance of the violation sample homogeneity loophole must be closed. Therefore we repeat after Jeffrey T.Leek and Roger D.Peng that sample homogeneity loophole is probably just the ...
Phungamngoen Chanthima; Asawajinda Tippunsa; Santad Rujira; Sawedboworn Wanticha
2016-01-01
Coconut milk is one of the most important protein-rich food sources available today. Separation of an emulsion into an aqueous phase and cream phase is commonly occurred and this leads an unacceptably physical defect of either fresh or processed coconut milk. Since homogenization steps are known to affect the stability of coconut milk. This work was aimed to study the effect of homogenization steps on quality of coconut milk. The samples were subject to high speed homogenization in the range ...
Ahmad, R.
2016-07-01
This article reports an unbiased analysis for the water based rod shaped alumina nanoparticles by considering both the homogeneous and non-homogeneous nanofluid models over the coupled nanofluid-surface interface. The mechanics of the surface are found for both the homogeneous and non-homogeneous models, which were ignored in previous studies. The viscosity and thermal conductivity data are implemented from the international nanofluid property benchmark exercise. All the simulations are being done by using the experimentally verified results. By considering the homogeneous and non-homogeneous models, the precise movement of the alumina nanoparticles over the surface has been observed by solving the corresponding system of differential equations. For the non-homogeneous model, a uniform temperature and nanofluid volume fraction are assumed at the surface, and the flux of the alumina nanoparticle is taken as zero. The assumption of zero nanoparticle flux at the surface makes the non-homogeneous model physically more realistic. The differences of all profiles for both the homogeneous and nonhomogeneous models are insignificant, and this is due to small deviations in the values of the Brownian motion and thermophoresis parameters.
Homogenized thermal conduction model for particulate foods
Chinesta, Francisco [Laboratoire de mecanique des systemes et des procedes, Ecole nationale superieure d' arts et metiers, 151 boulevard de l' Hopital, 75013, Paris (France); Torres, Rafael [Departamento de Ingenieria Mecanica, Universidad Politecnica de Valencia, Camino de Vera s/n. 46071, Valencia (Spain); Ramon, Antonio [AIMPLAS, Gustave Eiffel 4, 46980 Paterna, Valencia (Spain); Rodrigo, Mari Carmen; Rodrigo, Miguel [Instituto de Agroquimica y Tecnologia de Alimentos, Consejo Superior de Investigaciones Cientificas, Apartado de correos 73, 46100, Burjasot (Spain)
2002-12-01
This paper deals with the definition of an equivalent thermal conductivity for particulate foods. An homogenized thermal model is used to asses the effect of particulate spatial distribution and differences in thermal conductivities. We prove that the spatial average of the conductivity can be used in an homogenized heat transfer model if the conductivity differences among the food components are not very large, usually the highest conductivity ratio between the foods components is lower than 5. In the general case we propose to use a standard spatial homogenization procedure. Although the heterogeneity give rise to an anisotropic heat transfer behaviour, this effect is negligible when the food particles are randomly distributed. When we use pre-mixed particulate foods a statistical average can be defined from a small number of possible particle arrangements. (authors)
Homogenity of Die Casting and Returning Material
J. Malik
2012-04-01
Full Text Available Homogeneity of die castings is influenced by wide range of technological parameters as piston velocity in filling chamber of die casting machine, filling time of mould cavity, temperature of cast alloy, temperature of the mould, temperature of filling chamber, surface pressure on alloy during mould filling, final pressure and others. Based on stated parameters it is clear, that main parameters of die casting are filling time of die mould cavity and velocity of the melt in the ingates. Filling time must ensure the complete filling of the mould cavity before solidification process can negatively influence it. Among technological parameters also belong the returning material, which ratio in charge must be constrained according to requirement on final homogeneity of die castings. With the ratio of returning material influenced are the mechanical properties of castings, inner homogeneity and chemical composition.
Commensurability effects in holographic homogeneous lattices
Andrade, Tomas
2015-01-01
An interesting application of the gauge/gravity duality to condensed matter physics is the description of a lattice via breaking translational invariance on the gravity side. By making use of global symmetries, it is possible to do so without scarifying homogeneity of the pertinent bulk solutions, which we thus term as "homogeneous holographic lattices." Due to their technical simplicity, these configurations have received a great deal of attention in the last few years and have been shown to correctly describe momentum relaxation and hence (finite) DC conductivities. However, it is not clear whether they are able to capture other lattice effects which are of interest in condensed matter. In this paper we investigate this question focusing our attention on the phenomenon of commensurability, which arises when the lattice scale is tuned to be equal to (an integer multiple of) another momentum scale in the system. We do so by studying the formation of spatially modulated phases in various models of homogeneous ...
New applications of the homogeneous balance principle
张金良; 王跃明; 王明亮; 方宗德
2003-01-01
The homogeneous balance principle has been widely applied to the exploration of nonlinear transformation, exact solutions (especially solitary wave solution), dromion and similarity reduction to the nonlinear partial differential equations in mathematical physics. In this paper, we use the homogeneous balance principle to derive Backlund transformations for nonlinear partial differential equations that have more nonlinear terms and more highest-order partial derivative terms. With the aid of the Backlund transformations derived here, we could obtain exact solutions to the nonlinear partial differential equations. The Davey-Stewartson equation and the Nizhnik-Novikov-Veselov equation are considered as the examples.
Homogenization of High-Contrast Brinkman Flows
Brown, Donald L.
2015-04-16
Modeling porous flow in complex media is a challenging problem. Not only is the problem inherently multiscale but, due to high contrast in permeability values, flow velocities may differ greatly throughout the medium. To avoid complicated interface conditions, the Brinkman model is often used for such flows [O. Iliev, R. Lazarov, and J. Willems, Multiscale Model. Simul., 9 (2011), pp. 1350--1372]. Instead of permeability variations and contrast being contained in the geometric media structure, this information is contained in a highly varying and high-contrast coefficient. In this work, we present two main contributions. First, we develop a novel homogenization procedure for the high-contrast Brinkman equations by constructing correctors and carefully estimating the residuals. Understanding the relationship between scales and contrast values is critical to obtaining useful estimates. Therefore, standard convergence-based homogenization techniques [G. A. Chechkin, A. L. Piatniski, and A. S. Shamev, Homogenization: Methods and Applications, Transl. Math. Monogr. 234, American Mathematical Society, Providence, RI, 2007, G. Allaire, SIAM J. Math. Anal., 23 (1992), pp. 1482--1518], although a powerful tool, are not applicable here. Our second point is that the Brinkman equations, in certain scaling regimes, are invariant under homogenization. Unlike in the case of Stokes-to-Darcy homogenization [D. Brown, P. Popov, and Y. Efendiev, GEM Int. J. Geomath., 2 (2011), pp. 281--305, E. Marusic-Paloka and A. Mikelic, Boll. Un. Mat. Ital. A (7), 10 (1996), pp. 661--671], the results presented here under certain velocity regimes yield a Brinkman-to-Brinkman upscaling that allows using a single software platform to compute on both microscales and macroscales. In this paper, we discuss the homogenized Brinkman equations. We derive auxiliary cell problems to build correctors and calculate effective coefficients for certain velocity regimes. Due to the boundary effects, we construct
Small codimension subvarieties in homogeneous spaces
Perrin, Nicolas
2010-01-01
We prove Bertini type theorems for the inverse image, under a proper morphism, of any Schubert variety in an homogeneous space. Using generalisations of Deligne's trick, we deduce connectedness results for the inverse image of the diagonal in $X^2$ where $X$ is any isotropic grassmannian. We also deduce simple connectedness properties for subvarieties of $X$. Finally we prove transplanting theorems {\\`a} la Barth-Larsen for the Picard group of any isotropic grassmannian of lines and for the Neron-Severi group of some adjoint and coadjoint homogeneous spaces.
Study on Homogeneous Particleboard of Wheat Straw
无
2003-01-01
In the paper homogeneous particleboard of wheat straw is researched. The result shows the technology of homogeneous particleboard from cost and quality. The moisture content of straw particle is 2.0%~2.5 %. The temperature of hot-pressing is 150℃. The time of hot-pressing is 48 sec/mm ( panel thickness). The ratio between MDI and UF is 0.40. The glue content for surface layer of wheat straw particle is 10% (MDI 2.86%, UF 7.14%). The glue content for core layer of wheat straw particle is 8% (MDI 2.29%, U...
On gain in homogenized composite materials
Mackay, Tom G.; Lakhtakia, Akhlesh
2016-09-01
Three theoretical studies were undertaken, each based on the Bruggeman homogenization formalism and each involving homogenized composite materials (HCMs) comprising active component materials. It was found that: (i) HCMs can exhibit higher degrees of amplification than are exhibited by the HCM's component materials; (ii) anisotropic HCMs can simultaneously exhibit plane-wave amplification for certain propagation directions and plane-wave attenuation for other propagation directions; and (iii) for isotropic chiral HCMs, left-circularly polarized fields may be amplified while right-circularly polarized fields may be simultaneously attenuated (or vice versa) in any propagation direction.
An eigenelement method and two homogenization conditions
Yufeng Xing; Xingming Wang
2009-01-01
Under inspiration from the structure-preserving property of symplectic difference schemes for Hamiltonian systems, two homogenization conditions for a representa-tive unit cell of the periodical composites are proposed, one condition is the equivalence of strain energy, and the other is the deformation similarity. Based on these two homoge-nization conditions, an eigenelement method is presented, which is characteristic of structure-preserving property. It follows from the frequency comparisons that the eigenel-ement method is more accurate than the stiffness average method and the compliance average method.
Flows and chemical reactions in homogeneous mixtures
Prud'homme, Roger
2013-01-01
Flows with chemical reactions can occur in various fields such as combustion, process engineering, aeronautics, the atmospheric environment and aquatics. The examples of application chosen in this book mainly concern homogeneous reactive mixtures that can occur in propellers within the fields of process engineering and combustion: - propagation of sound and monodimensional flows in nozzles, which may include disequilibria of the internal modes of the energy of molecules; - ideal chemical reactors, stabilization of their steady operation points in the homogeneous case of a perfect mixture and c
Statistical methods for assessment of blend homogeneity
Madsen, Camilla
2002-01-01
In this thesis the use of various statistical methods to address some of the problems related to assessment of the homogeneity of powder blends in tablet production is discussed. It is not straight forward to assess the homogeneity of a powder blend. The reason is partly that in bulk materials......, it is shown how to set up parametric acceptance criteria for the batch that gives a high confidence that future samples with a probability larger than a specified value will pass the USP threeclass criteria. Properties and robustness of proposed changes to the USP test for content uniformity are investigated...
Frenod, Emmanuel
2013-01-01
In this note, a classification of Homogenization-Based Numerical Methods and (in particular) of Numerical Methods that are based on the Two-Scale Convergence is done. In this classification stand: Direct Homogenization-Based Numerical Methods; H-Measure-Based Numerical Methods; Two-Scale Numerical Methods and TSAPS: Two-Scale Asymptotic Preserving Schemes.
Hyperelastic bodies under homogeneous Cauchy stress induced by non-homogeneous finite deformations
Mihai, L Angela
2016-01-01
We discuss whether homogeneous Cauchy stress implies homogeneous strain in isotropic nonlinear elasticity. While for linear elasticity the positive answer is clear, we exhibit, through detailed calculations, an example with inhomogeneous continuous deformation but constant Cauchy stress. The example is derived from a non rank-one convex elastic energy. Connections to conforming and non-conforming finite element implementations are drawn.
Microbead agglutination based assays
Kodzius, Rimantas
2013-01-21
We report a simple and rapid room temperature assay for point-of-care (POC) testing that is based on specific agglutination. Agglutination tests are based on aggregation of microbeads in the presence of a specific analyte thus enabling the macroscopic observation. Such tests are most often used to explore antibody-antigen reactions. Agglutination has been used for protein assays using a biotin/streptavidin system as well as a hybridization based assay. The agglutination systems are prone to selftermination of the linking analyte, prone to active site saturation and loss of agglomeration at high analyte concentrations. We investigated the molecular target/ligand interaction, explaining the common agglutination problems related to analyte self-termination, linkage of the analyte to the same bead instead of different microbeads. We classified the agglutination process into three kinds of assays: a two- component assay, a three-component assay and a stepped three- component assay. Although we compared these three kinds of assays for recognizing DNA and protein molecules, the assay can be used for virtually any molecule, including ions and metabolites. In total, the optimized assay permits detecting analytes with high sensitivity in a short time, 5 min, at room temperature. Such a system is appropriate for POC testing.
Colorimetric protein assay techniques.
Sapan, C V; Lundblad, R L; Price, N C
1999-04-01
There has been an increase in the number of colorimetric assay techniques for the determination of protein concentration over the past 20 years. This has resulted in a perceived increase in sensitivity and accuracy with the advent of new techniques. The present review considers these advances with emphasis on the potential use of such technologies in the assay of biopharmaceuticals. The techniques reviewed include Coomassie Blue G-250 dye binding (the Bradford assay), the Lowry assay, the bicinchoninic acid assay and the biuret assay. It is shown that each assay has advantages and disadvantages relative to sensitivity, ease of performance, acceptance in the literature, accuracy and reproducibility/coefficient of variation/laboratory-to-laboratory variation. A comparison of the use of several assays with the same sample population is presented. It is suggested that the most critical issue in the use of a chromogenic protein assay for the characterization of a biopharmaceutical is the selection of a standard for the calibration of the assay; it is crucial that the standard be representative of the sample. If it is not possible to match the standard with the sample from the perspective of protein composition, then it is preferable to use an assay that is not sensitive to the composition of the protein such as a micro-Kjeldahl technique, quantitative amino acid analysis or the biuret assay. In a complex mixture it might be inappropriate to focus on a general method of protein determination and much more informative to use specific methods relating to the protein(s) of particular interest, using either specific assays or antibody-based methods. The key point is that whatever method is adopted as the 'gold standard' for a given protein, this method needs to be used routinely for calibration.
Mukherjee, Sourav; Alicia M Hanson; Shadrick, William R.; Ndjomou, Jean; Sweeney, Noreena L.; Hernandez, John J.; Bartczak, Diana; Li, Kelin; Frankowski, Kevin J.; Heck, Julie A.; Arnold, Leggy A.; Schoenen, Frank J.; Frick, David N.
2012-01-01
Typical assays used to discover and analyze small molecules that inhibit the hepatitis C virus (HCV) NS3 helicase yield few hits and are often confounded by compound interference. Oligonucleotide binding assays are examined here as an alternative. After comparing fluorescence polarization (FP), homogeneous time-resolved fluorescence (HTRF®; Cisbio) and AlphaScreen® (Perkin Elmer) assays, an FP-based assay was chosen to screen Sigma’s Library of Pharmacologically Active Compounds (LOPAC) for c...
A DNA-Mediated Homogeneous Binding Assay for Proteins and Small Molecules
Zhang, Zhao; Hejesen, Christian; Kjelstrup, Michael Brøndum
2014-01-01
Optical detection of molecular targets typically requires immobilization, separation, or chemical or enzymatic processing. An important exception is aptamers that allow optical detection in solution based on conformational changes. This method, however, requires the laborious selection of aptamers...... with high target specificity and affinity, and the ability to undergo the required conformational changes. Here we report on an alternative generic scheme for detecting small molecules and proteins in solution based on a shift in the equilibrium of DNA-based strand displacement competition reaction....... The shift occurs upon binding of a protein, for example, an antibody to its target. We demonstrate nanomolar detection of small molecules such as biotin, digoxigenin, vitamin D, and folate, in buffer and in plasma. The method is flexible, and we also show nanomolar detection of the respective antibodies...
Inverse acoustic problem of N homogeneous scatterers
Berntsen, Svend
2002-01-01
The three-dimensional inverse acoustic medium problem of N homogeneous objects with known geometry and location is considered. It is proven that one scattering experiment is sufficient for the unique determination of the complex wavenumbers of the objects. The mapping from the scattered fields...
Delay differential equations with homogeneous integral conditions
Abdur Raheem
2013-03-01
Full Text Available In this article we prove the existence and uniqueness of a strong solution of a delay differential equation with homogenous integral conditions using the method of semidiscretization in time. As an application, we include an example that illustrates the main result.
On the Cancellation Rule in the Homogenization
Victor Ufnarovski
2008-04-01
Full Text Available We consider the possible ways of the homogenization of non-graded non-commutative algebra and show that it should be combined with the cancellation rule to get the mathematically adequate correspondence between graded and non-graded algebras.
Locally homogeneous structures on Hopf surfaces
McKay, Benjamin
2009-01-01
We study holomorphic locally homogeneous geometric structures modelled on line bundles over the projective line. We classify these structures on primary Hopf surfaces. We write out the developing map and holonomy morphism of each of these structures explicitly on each primary Hopf surface.
Homogeneous Catalysis by Transition Metal Compounds.
Mawby, Roger
1988-01-01
Examines four processes involving homogeneous catalysis which highlight the contrast between the simplicity of the overall reaction and the complexity of the catalytic cycle. Describes how catalysts provide circuitous routes in which all energy barriers are relatively low rather than lowering the activation energy for a single step reaction.…
On chiral differential operators over homogeneous spaces
Vassily Gorbounov
2001-01-01
Full Text Available We give a classification and construction of chiral algebras of differential operators over semisimple algebraic groups G and over homogeneous spaces G/N and G/P where N is a nilpotent and P a parabolic subgroup.
String pair production in non homogeneous backgrounds
Bolognesi, Stefano; Tallarita, Gianni
2016-01-01
We consider string pair production in non homogeneous electric backgrounds. We study several particular configurations which can be addressed with the Euclidean world-sheet instanton technique, the analogue of the world-line instanton for particles. In the first case the string is suspended between two D-branes in flat space-time, in the second case the string lives in AdS and terminates on one D-brane (this realizes the holographic Schwinger effect). In some regions of parameter space the result is well approximated by the known analytical formulas, either the particle pair production in non-homogeneous background or the string pair production in homogeneous background. In other cases we see effects which are intrinsically stringy and related to the non-homogeneity of the background. The pair production is enhanced already for particles in time dependent electric field backgrounds. The string nature enhances this even further. For spacial varying electrical background fields the string pair production is les...
The homogeneous marginal utility of income assumption
Demuynck, T.
2015-01-01
We develop a test to verify if every agent from a population of heterogeneous consumers has the same marginal utility of income function. This homogeneous marginal utility of income assumption is often (implicitly) used in applied demand studies because it has nice aggregation properties and facilit
Remarks on homogeneous manifolds satisfying Levi conditions
Huckleberry, Alan
2010-01-01
Homogeneous complex manifolds satisfying various types of Levi conditions are considered. Classical results which were of particular interest to Andreotti are recalled. Convexity and concavity properties of flag domains are discussed in some detail. A precise classification of pseudoconvex flag domains is given. It is shown that flag domains which are in a certain sense generic are pseudoconcave.
Homogeneity in Social Groups of Iraqis
Gresham, J.; Saleh, F.; Majid, S.
With appreciation to the Royal Institute for Inter-Faith Studies for initiating the Second World Congress for Middle Eastern Studies, this paper summarizes findings on homogeneity in community-level social groups derived from inter-ethnic research conducted during 2005 among Iraqi Arabs and Kurds
Inverse acoustic problem of N homogeneous scatterers
Berntsen, Svend
2002-01-01
The three-dimensional inverse acoustic medium problem of N homogeneous objects with known geometry and location is considered. It is proven that one scattering experiment is sufficient for the unique determination of the complex wavenumbers of the objects. The mapping from the scattered fields...
Local real analysis in locally homogeneous spaces
Bramanti, Marco
2011-01-01
We introduce the concept of locally homogeneous space, and prove in this context L^p and Holder estimates for singular and fractional integrals, as well as L^p estimates on the commutator of a singular or fractional integral with a BMO or VMO function. These results are motivated by local a-priori estimates for subelliptic equations.
Homogeneity in Social Groups of Iraqis
Gresham, J.; Saleh, F.; Majid, S.
2006-01-01
With appreciation to the Royal Institute for Inter-Faith Studies for initiating the Second World Congress for Middle Eastern Studies, this paper summarizes findings on homogeneity in community-level social groups derived from inter-ethnic research conducted during 2005 among Iraqi Arabs and Kurds li
Absolute nuclear material assay
Prasad, Manoj K [Pleasanton, CA; Snyderman, Neal J [Berkeley, CA; Rowland, Mark S [Alamo, CA
2012-05-15
A method of absolute nuclear material assay of an unknown source comprising counting neutrons from the unknown source and providing an absolute nuclear material assay utilizing a model to optimally compare to the measured count distributions. In one embodiment, the step of providing an absolute nuclear material assay comprises utilizing a random sampling of analytically computed fission chain distributions to generate a continuous time-evolving sequence of event-counts by spreading the fission chain distribution in time.
Phungamngoen Chanthima
2016-01-01
Full Text Available Coconut milk is one of the most important protein-rich food sources available today. Separation of an emulsion into an aqueous phase and cream phase is commonly occurred and this leads an unacceptably physical defect of either fresh or processed coconut milk. Since homogenization steps are known to affect the stability of coconut milk. This work was aimed to study the effect of homogenization steps on quality of coconut milk. The samples were subject to high speed homogenization in the range of 5000-15000 rpm under sterilize temperatures at 120-140 °C for 15 min. The result showed that emulsion stability increase with increasing speed of homogenization. The lower fat particles were generated and easy to disperse in continuous phase lead to high stability. On the other hand, the stability of coconut milk decreased, fat globule increased, L value decreased and b value increased when the high sterilization temperature was applied. Homogenization after heating led to higher stability than homogenization before heating due to the reduced particle size of coconut milk after aggregation during sterilization process. The results implied that homogenization after sterilization process might play an important role on the quality of the sterilized coconut milk.
Moore, Evan G.; Samuel, Amanda P. S.; Raymond, Kenneth N.
2009-01-01
Conspectus Ligand-sensitized, luminescent lanthanide(III) complexes are of considerable importance because their unique photophysical properties (microsecond to millisecond lifetimes, characteristic and narrow emission bands, and large Stokes shifts) make them well suited as labels in fluorescence-based bioassays. The long-lived emission of lanthanide(III) cations can be temporally resolved from scattered light and background fluorescence to vastly enhance measurement sensitivity. One challenge in this field is the design of sensitizing ligands that provide highly emissive complexes with sufficient stability and aqueous solubility for practical applications. In this Account, we give an overview of some of the general properties of the trivalent lanthanides and follow with a summary of advances made in our laboratory in the development of highly luminescent Tb(III) and Eu(III) complexes for applications in biotechnology. A focus of our research has been the optimization of these compounds as potential commercial agents for use in Homogeneous Time-Resolved Fluorescence (HTRF) technology. Our approach involves developing high-stability octadentate Tb(III) and Eu(III) complexes that rely on all-oxygen donor atoms and using multi-chromophore chelates to increase molar absorptivity; earlier examples utilized a single pendant chromophore (that is, a single “antenna”). Ligands based on 2-hydroxyisophthalamide (IAM) provide exceptionally emissive Tb(III) complexes with quantum yield values up to ∼60% that are stable at the nanomolar concentrations required for commercial assays. Through synthetic modification of the IAM chromophore and time-dependent density functional theory (TD-DFT) calculations, we have developed a method to predict absorption and emission properties of these chromophores as a tool to guide ligand design. Additionally, we have investigated chiral IAM ligands that yield Tb(III) complexes possessing both high quantum yield values and strong
Jiang, Chao; Yuan, Yuan; Liu, Libing; Hou, Jingyi; Jin, Yan; Huang, Luqi
2015-01-01
A label-free, homogenous and sensitive one-step method for the molecular authentication of medicinal snakes has been developed by combining a rapid PCR technique with water-soluble cationic conjugated polyelectrolytes (CCPs). Three medicinal snake materials (Deinagkistrodon acutus, Zaocys dhumnades and Bungarus multicinctus; a total of 35 specimens) and 48 snake specimens with similar morphologies and textures were clearly distinguished by the naked eye by utilizing a CCP-based assay in a high-throughput manner. The identification of medicinal snakes in patented Chinese drugs was successfully performed using this detection system. In contrast to previous fluorescence-labeled oligonucleotide detection and direct DNA stain hybridization assays, this method does not require designing dye-labeled primers, and unfavorable dimer fluorescence is avoided in this homogenous method. PMID:26537289
Jiang, Chao; Yuan, Yuan; Liu, Libing; Hou, Jingyi; Jin, Yan; Huang, Luqi
2015-11-05
A label-free, homogenous and sensitive one-step method for the molecular authentication of medicinal snakes has been developed by combining a rapid PCR technique with water-soluble cationic conjugated polyelectrolytes (CCPs). Three medicinal snake materials (Deinagkistrodon acutus, Zaocys dhumnades and Bungarus multicinctus; a total of 35 specimens) and 48 snake specimens with similar morphologies and textures were clearly distinguished by the naked eye by utilizing a CCP-based assay in a high-throughput manner. The identification of medicinal snakes in patented Chinese drugs was successfully performed using this detection system. In contrast to previous fluorescence-labeled oligonucleotide detection and direct DNA stain hybridization assays, this method does not require designing dye-labeled primers, and unfavorable dimer fluorescence is avoided in this homogenous method.
Jun Kobayashi
2015-01-01
Full Text Available We propose a robust method to distinguish isolated single gold nanoparticles (AuNP monomers and their dimers under Brownian motion, a key for ultrasensitive homogeneous bioassays, including AuNP sandwich assays. To detect dimers and distinguish them from a larger number of monomers in aqueous solution, single-particle polarization microscopy was performed. For the accurate detection of individual particles, the optical anisotropy and rotational diffusion time are measured because a dimer is much more anisotropic than the nearly spherical monomer and the rotational diffusion time of a dimer is four times that of a monomer. By employing an autocorrelation analysis, we defined a measure of distinguishing that simultaneously enables high detection probability and low error probability. The detection platform offers homogeneous DNA hybridization assays and immunoassays at the subpicomolar level.
Some dimensional results for homogeneous Moran sets
丰德军; 文志英; 吴军
1997-01-01
Let M({nk}k≥1,{ck}k≥1) be the collection of homogeneous Moran sets determined by {nk}k≥1and {ck}k≥1, where {nk}k≥1 is a sequence of positive integers and {ck}k≥1 a sequence of positive numbers. Then the maximal and minimal values of Hausdorff dimensions for elements in M are determined. The result is proved that for any value s between the maximal and minimal values, there exists an element in M{nk}k≥1, {ck}k≥1) such that its Hausdorff dimension is equal to s. The same results hold for packing dimension. In the meantime, some other properties of homogeneous Moran sets are discussed.
CUDA Simulation of Homogeneous, Incompressible Turbulence
Morin, Lee; Shebalin, John V.; Shum, Victor; Fu, Terry
2011-01-01
We discuss very fast Compute Unified Device Architecture (CUDA) simulations of ideal homogeneous incompressible turbulence based on Fourier models. These models have associated statistical theories that predict that Fourier coefficients of fluid velocity and magnetic fields (if present) are zero-mean random variables. Prior numerical simulations have shown that certain coefficients have a non-zero mean value that can be very large compared to the associated standard deviation. We review the theoretical basis of this "broken ergodicity" as applied to 2-D and 3-D fluid and magnetohydrodynamic simulations of homogeneous turbulence. Our new simulations examine the phenomenon of broken ergodicity through very long time and large grid size runs performed on a state-of-the-art CUDA platform. Results comparing various CUDA hardware configurations and grid sizes are discussed. NS and MHD results are compared.
Kinematical uniqueness of homogeneous isotropic LQC
Engle, Jonathan; Hanusch, Maximilian
2017-01-01
In a paper by Ashtekar and Campiglia, invariance under volume preserving residual diffeomorphisms has been used to single out the standard representation of the reduced holonomy-flux algebra in homogeneous loop quantum cosmology (LQC). In this paper, we use invariance under all residual diffeomorphisms to single out the standard kinematical Hilbert space of homogeneous isotropic LQC for both the standard configuration space {{{R}}\\text{Bohr}} , as well as for the Fleischhack one {R}\\sqcup {{{R}}\\text{Bohr}} . We first determine the scale invariant Radon measures on these spaces, and then show that the Haar measure on {{{R}}\\text{Bohr}} is the only such measure for which the momentum operator is hermitian w.r.t. the corresponding inner product. In particular, the measure is forced to be identically zero on {R} in the Fleischhack case, so that for both approaches, the standard kinematical LQC-Hilbert space is singled out.
Broken Ergodicity in Ideal, Homogeneous, Incompressible Turbulence
Morin, Lee; Shebalin, John; Fu, Terry; Nguyen, Phu; Shum, Victor
2010-01-01
We discuss the statistical mechanics of numerical models of ideal homogeneous, incompressible turbulence and their relevance for dissipative fluids and magnetofluids. These numerical models are based on Fourier series and the relevant statistical theory predicts that Fourier coefficients of fluid velocity and magnetic fields (if present) are zero-mean random variables. However, numerical simulations clearly show that certain coefficients have a non-zero mean value that can be very large compared to the associated standard deviation. We explain this phenomena in terms of broken ergodicity', which is defined to occur when dynamical behavior does not match ensemble predictions on very long time-scales. We review the theoretical basis of broken ergodicity, apply it to 2-D and 3-D fluid and magnetohydrodynamic simulations of homogeneous turbulence, and show new results from simulations using GPU (graphical processing unit) computers.
Coupled quasi-homogeneous orthotropic laminates
York, C. B.
2011-09-01
Laminate stacking sequence configurations with quasi-homogeneous properties are derived, whereby the inplane and out-of-plane stiffness properties are concomitant. The stacking sequence configurations contain equal-thickness layers and identical orthotropic properties, differing only by their orientations, which are represented by standard angle- and cross-ply combinations. These simplifying assumptions are in fact representative of the common design practice, but help one to identify and isolate a range of highly complex physical coupling responses, which continue to be referred to collectively in the literature as bending-extension coupling. Dimensionless parameters are developed from which the elements of the extensional, coupling, and bending stiffness matrices are readily calculated for any fibre/resin properties. Feasible domains of lamination parameters are also illustrated for each coupling response to complement abridged listings of stacking sequences. Finally, hygrothermally curvature-stable configurations with quasi-homogeneous properties are identified, thus simplifying the manufacture of laminate configurations possessing tailored mechanical coupling responses.
Searching for the scale of homogeneity
Martínez, V J; Moyeed, R A; Graham, M J; Martinez, Vicent J.; Pons-Borderia, Maria-Jesus; Moyeed, Rana A.; Graham, Matthew J.
1998-01-01
We introduce a statistical quantity, known as the $K$ function, related to the integral of the two--point correlation function. It gives us straightforward information about the scale where clustering dominates and the scale at which homogeneity is reached. We evaluate the correlation dimension, $D_2$, as the local slope of the log--log plot of the $K$ function. We apply this statistic to several stochastic point fields, to three numerical simulations describing the distribution of clusters and finally to real galaxy redshift surveys. Four different galaxy catalogues have been analysed using this technique: the Center for Astrophysics I, the Perseus--Pisces redshift surveys (these two lying in our local neighbourhood), the Stromlo--APM and the 1.2 Jy {\\it IRAS} redshift surveys (these two encompassing a larger volume). In all cases, this cumulant quantity shows the fingerprint of the transition to homogeneity. The reliability of the estimates is clearly demonstrated by the results from controllable point sets...
Kinematical uniqueness of homogeneous isotropic LQC
Engle, Jonathan
2016-01-01
In a paper by Ashtekar and Campiglia, invariance under volume preserving residual diffeomorphisms has been used to single out the standard representation of the reduced holonomy-flux algebra in homogeneous loop quantum cosmology (LQC). In this paper, we use invariance under all residual diffeomorphisms to single out the standard kinematical Hilbert space of homogeneous isotropic LQC for both the standard configuration space $\\mathbb{R}_{\\mathrm{Bohr}}$, as well as for the Fleischhack one $\\mathbb{R} \\sqcup \\mathbb{R}_{\\mathrm{Bohr}}$. We first determine the scale invariant Radon measures on these spaces, and then show that the Haar measure on $\\mathbb{R}_{\\mathrm{Bohr}}$ is the only such measure for which the momentum operator is hermitian w.r.t. the corresponding inner product. In particular, the measure is forced to be identically zero on $\\mathbb{R}$ in the Fleischhack case, so that for both approaches, the standard kinematical LQC-Hilbert space is singled out.
Program Logics for Homogeneous Meta-programming
Berger, Martin; Tratt, Laurence
A meta-program is a program that generates or manipulates another program; in homogeneous meta-programming, a program may generate new parts of, or manipulate, itself. Meta-programming has been used extensively since macros were introduced to Lisp, yet we have little idea how formally to reason about meta-programs. This paper provides the first program logics for homogeneous meta-programming - using a variant of MiniML_e^{square} by Davies and Pfenning as underlying meta-programming language. We show the applicability of our approach by reasoning about example meta-programs from the literature. We also demonstrate that our logics are relatively complete in the sense of Cook, enable the inductive derivation of characteristic formulae, and exactly capture the observational properties induced by the operational semantics.
Statistical methods for assessment of blend homogeneity
Madsen, Camilla
2002-01-01
as powder blends there is no natural unit or amount to define a sample from the blend, and partly that current technology does not provide a method of universally collecting small representative samples from large static powder beds. In the thesis a number of methods to assess (in)homogeneity are presented...... of internal factors to the blend e.g. the particle size distribution. The relation between particle size distribution and the variation in drug content in blend and tablet samples is discussed. A central problem is to develop acceptance criteria for blends and tablet batches to decide whether the blend...... blend or batch. In the thesis it is shown how to link sampling result and acceptance criteria to the actual quality (homogeneity) of the blend or tablet batch. Also it is discussed how the assurance related to a specific acceptance criteria can be obtained from the corresponding OC-curve. Further...
Metric Diophantine approximation on homogeneous varieties
Ghosh, Anish; Nevo, Amos
2012-01-01
We develop the metric theory of Diophantine approximation on homogeneous varieties of semisimple algebraic groups and prove results analogous to the classical Khinchin and Jarnik theorems. In full generality our results establish simultaneous Diophantine approximation with respect to several completions, and Diophantine approximation over general number fields using S-algebraic integers. In several important examples, the metric results we obtain are optimal. The proof uses quantitative equidistribution properties of suitable averaging operators, which are derived from spectral bounds in automorphic representations.
Correlated equilibria in homogenous good Bertrand competition
Jann, Ole; Schottmüller, Christoph
2015-01-01
We show that there is a unique correlated equilibrium, identical to the unique Nash equilibrium, in the classic Bertrand oligopoly model with homogenous goods and identical marginal costs. This provides a theoretical underpinning for the so-called "Bertrand paradox'' as well as its most general f...... formulation to date. Our proof generalizes to asymmetric marginal costs and arbitrarily many players in the following way: The market price cannot be higher than the second lowest marginal cost in any correlated equilibrium....
Higher dimensional homogeneous cosmology in Lyra geometry
F Rahaman; S Das; N Begum; M Hossain
2003-07-01
Assuming a homogeneous perfect ﬂuid with ρ = ρ() and = (), we have obtained exact solutions for cosmological models in higher-dimension based on Lyra geometry. Depending on the form of metric chosen, the model is similar to FRW type. The explicit solutions of the scale factor are found via the assumption of an equation of state = ρ, where is a constant. Some astrophysical parameters are also calculated.
HSTEP -- Homogeneous Studies of Transiting Extrasolar Planets
Southworth, John
2014-01-01
I give a summary of the HSTEP project: an effort to calculate the physical properties of the known transiting extrasolar planets using a homogeneous approach. I discuss the motivation for the project, list the 83 planets which have already been studied, run through some important aspects of the methodology, and finish with a synopsis of the results. The results have been compiled into an online catalogue: TEPCat.
Recent advances in homogeneous nickel catalysis.
Tasker, Sarah Z; Standley, Eric A; Jamison, Timothy F
2014-05-15
Tremendous advances have been made in nickel catalysis over the past decade. Several key properties of nickel, such as facile oxidative addition and ready access to multiple oxidation states, have allowed the development of a broad range of innovative reactions. In recent years, these properties have been increasingly understood and used to perform transformations long considered exceptionally challenging. Here we discuss some of the most recent and significant developments in homogeneous nickel catalysis, with an emphasis on both synthetic outcome and mechanism.
Tits Satake projections of homogeneous special geometries
Fré, Pietro; Gargiulo, Floriana; Rosseel, Jan; Rulik, Ksenya; Trigiante, Mario; Van Proeyen, Antoine
2007-01-01
We organize the homogeneous special geometries, describing as well the couplings of D = 6, 5, 4 and 3 supergravities with eight supercharges, in a small number of universality classes. This relates manifolds on which similar types of dynamical solutions can exist. The mathematical ingredient is the Tits Satake projection of real simple Lie algebras, which we extend to all solvable Lie algebras occurring in these homogeneous special geometries. Apart from some exotic cases all the other, 'very special', homogeneous manifolds can be grouped into seven universality classes. The organization of these classes, which capture the essential features of their basic dynamics, commutes with the r- and c-map. Different members are distinguished by different choices of the paint group, a notion discovered in the context of cosmic billiard dynamics of non-maximally supersymmetric supergravities. We comment on the usefulness of this organization in universality class both in relation with cosmic billiard dynamics and with configurations of branes and orbifolds defining special geometry backgrounds.
Equilibrium states of homogeneous sheared compressible turbulence
Riahi, M.; Lili, T.
2011-06-01
Equilibrium states of homogeneous compressible turbulence subjected to rapid shear is studied using rapid distortion theory (RDT). The purpose of this study is to determine the numerical solutions of unsteady linearized equations governing double correlations spectra evolution. In this work, RDT code developed by authors solves these equations for compressible homogeneous shear flows. Numerical integration of these equations is carried out using a second-order simple and accurate scheme. The two Mach numbers relevant to homogeneous shear flow are the turbulent Mach number Mt, given by the root mean square turbulent velocity fluctuations divided by the speed of sound, and the gradient Mach number Mg which is the mean shear rate times the transverse integral scale of the turbulence divided by the speed of sound. Validation of this code is performed by comparing RDT results with direct numerical simulation (DNS) of [A. Simone, G.N. Coleman, and C. Cambon, Fluid Mech. 330, 307 (1997)] and [S. Sarkar, J. Fluid Mech. 282, 163 (1995)] for various values of initial gradient Mach number Mg0. It was found that RDT is valid for small values of the non-dimensional times St (St 10) in particular for large values of Mg0. This essential feature justifies the resort to RDT in order to determine equilibrium states in the compressible regime.
Equilibrium states of homogeneous sheared compressible turbulence
M. Riahi
2011-06-01
Full Text Available Equilibrium states of homogeneous compressible turbulence subjected to rapid shear is studied using rapid distortion theory (RDT. The purpose of this study is to determine the numerical solutions of unsteady linearized equations governing double correlations spectra evolution. In this work, RDT code developed by authors solves these equations for compressible homogeneous shear flows. Numerical integration of these equations is carried out using a second-order simple and accurate scheme. The two Mach numbers relevant to homogeneous shear flow are the turbulent Mach number Mt, given by the root mean square turbulent velocity fluctuations divided by the speed of sound, and the gradient Mach number Mg which is the mean shear rate times the transverse integral scale of the turbulence divided by the speed of sound. Validation of this code is performed by comparing RDT results with direct numerical simulation (DNS of [A. Simone, G.N. Coleman, and C. Cambon, Fluid Mech. 330, 307 (1997] and [S. Sarkar, J. Fluid Mech. 282, 163 (1995] for various values of initial gradient Mach number Mg0. It was found that RDT is valid for small values of the non-dimensional times St (St 10 in particular for large values of Mg0. This essential feature justifies the resort to RDT in order to determine equilibrium states in the compressible regime.
Homogeneous Biosensing Based on Magnetic Particle Labels
Schrittwieser, Stefan
2016-06-06
The growing availability of biomarker panels for molecular diagnostics is leading to an increasing need for fast and sensitive biosensing technologies that are applicable to point-of-care testing. In that regard, homogeneous measurement principles are especially relevant as they usually do not require extensive sample preparation procedures, thus reducing the total analysis time and maximizing ease-of-use. In this review, we focus on homogeneous biosensors for the in vitro detection of biomarkers. Within this broad range of biosensors, we concentrate on methods that apply magnetic particle labels. The advantage of such methods lies in the added possibility to manipulate the particle labels by applied magnetic fields, which can be exploited, for example, to decrease incubation times or to enhance the signal-to-noise-ratio of the measurement signal by applying frequency-selective detection. In our review, we discriminate the corresponding methods based on the nature of the acquired measurement signal, which can either be based on magnetic or optical detection. The underlying measurement principles of the different techniques are discussed, and biosensing examples for all techniques are reported, thereby demonstrating the broad applicability of homogeneous in vitro biosensing based on magnetic particle label actuation.
TESTING HOMOGENEITY WITH GALAXY STAR FORMATION HISTORIES
Hoyle, Ben; Jimenez, Raul [Institut de Ciences del Cosmos (ICC), Universitat de Barcelona (IEEC-UB), Marti i Franques 1, E-08024 Barcelona (Spain); Tojeiro, Rita; Maartens, Roy [Institute of Cosmology and Gravitation, University of Portsmouth, Dennis Sciama Building, Portsmouth PO1 3FX (United Kingdom); Heavens, Alan [Imperial Centre for Inference and Cosmology, Astrophysics Group, Imperial College London, Blackett Laboratory, Prince Consort Road, London SW7 2AZ (United Kingdom); Clarkson, Chris [Astrophysics, Cosmology and Gravity Centre, and Department of Mathematics and Applied Mathematics, University of Cape Town, Rondebosch 7701 (South Africa)
2013-01-01
Observationally confirming spatial homogeneity on sufficiently large cosmological scales is of importance to test one of the underpinning assumptions of cosmology, and is also imperative for correctly interpreting dark energy. A challenging aspect of this is that homogeneity must be probed inside our past light cone, while observations take place on the light cone. The star formation history (SFH) in the galaxy fossil record provides a novel way to do this. We calculate the SFH of stacked luminous red galaxy (LRG) spectra obtained from the Sloan Digital Sky Survey. We divide the LRG sample into 12 equal-area contiguous sky patches and 10 redshift slices (0.2 < z < 0.5), which correspond to 120 blocks of volume {approx}0.04 Gpc{sup 3}. Using the SFH in a time period that samples the history of the universe between look-back times 11.5 and 13.4 Gyr as a proxy for homogeneity, we calculate the posterior distribution for the excess large-scale variance due to inhomogeneity, and find that the most likely solution is no extra variance at all. At 95% credibility, there is no evidence of deviations larger than 5.8%.
Cell viability assays: introduction.
Stoddart, Martin J
2011-01-01
The measurement of cell viability plays a fundamental role in all forms of cell culture. Sometimes it is the main purpose of the experiment, such as in toxicity assays. Alternatively, cell viability can be used to -correlate cell behaviour to cell number, providing a more accurate picture of, for example, anabolic -activity. There are wide arrays of cell viability methods which range from the most routine trypan blue dye exclusion assay to highly complex analysis of individual cells, such as using RAMAN microscopy. The cost, speed, and complexity of equipment required will all play a role in determining the assay used. This chapter aims to provide an overview of many of the assays available today.
Brown, Ryan M; Meah, Christopher J; Heath, Victoria L; Styles, Iain B; Bicknell, Roy
2016-01-01
Angiogenesis involves the generation of new blood vessels from the existing vasculature and is dependent on many growth factors and signaling events. In vivo angiogenesis is dynamic and complex, meaning assays are commonly utilized to explore specific targets for research into this area. Tube-forming assays offer an excellent overview of the molecular processes in angiogenesis. The Matrigel tube forming assay is a simple-to-implement but powerful tool for identifying biomolecules involved in angiogenesis. A detailed experimental protocol on the implementation of the assay is described in conjunction with an in-depth review of methods that can be applied to the analysis of the tube formation. In addition, an ImageJ plug-in is presented which allows automatic quantification of tube images reducing analysis times while removing user bias and subjectivity.
Transgenic Animal Mutation Assays
Tao Chen; Ph.D.D.A.B.T.
2005-01-01
@@ The novel transgenic mouse and rat mutation assays have provided a tool for analyzing in vivo mutation in any tissue, thus permitting the direct comparison of cancer incidence with mutant frequency.
McDonald, T.P.
1977-01-01
In summary, thrombopoietin levels have been determined indirectly by measuring thrombocytopoiesis in assay animals (platelet counting, measurement of isotope incorporation into newly formed platelets, changes in platelet sizes, or alterations in number and size of megakaryocytes) and by use of an immunoassay. Although much work remains, it seems clear at the present time that isotopic uptake into platelets of specially prepared assay mice (rebound-thrombocytosis) is superior to the other techniques now available for the measurement of thrombopoietin. However, the ideal assay for TSF which is specific, rapid, and inexpensive is yet to be developed. An immunoassay is in the development stage, but will require additional work before it can be utilized for the routine assay of TSF.
Lookeren Campagne, Michiel M. van; Erneux, Cristophe; Eijk, Ronald van; Haastert, Peter J.M. van
1988-01-01
Dictyostelium discoideum homogenates contain phosphatase activity which rapidly dephosphorylates Ins(1,4,5)P3 (D-myo-inositol 1,4,5-trisphosphate) to Ins (myo-inositol). When assayed in Mg2+, Ins(1,4,5)P3 is dephosphorylated by the soluble Dictyostelium cell fraction to 20% Ins(1,4)P2 (D-myo-inosito
Kminek, Gerhard; Conley, Catharine
2012-07-01
The presentation will detail approved Planetary Protection specifications for the Rapid Spore Assay for spacecraft components and subsystems. Outlined will be the research and studies on which the specifications were based. The research, funded by ESA and NASA/JPL, was conducted over a period of two years and was followed by limited cleanroom studies to assess the feasibility of this assay during spacecraft assembly.
REITERATED HOMOGENIZATION OF DEGENERATE NONLINEAR ELLIPTIC EQUATIONS
无
2002-01-01
The authors study homogenization of some nonlinear partial differential equations of the form -div (a (hx,h2x,Duh)) = f,where a is periodic in the first two arguments and monotone in the third.In particular the case where a satisfies degenerated structure conditions is studied.It is proved that uh converges weakly in Wo1.1 (Ω) to the unique solution of a limit problem as h →∞.Moreover,explicit expressions for the limit problem are obtained.
Droplet size distribution in homogeneous isotropic turbulence
Perlekar, Prasad; Biferale, Luca; Sbragaglia, Mauro; Srivastava, Sudhir; Toschi, Federico
2012-06-01
We study the physics of droplet breakup in a statistically stationary homogeneous and isotropic turbulent flow by means of high resolution numerical investigations based on the multicomponent lattice Boltzmann method. We verified the validity of the criterion proposed by Hinze [AIChE J. 1, 289 (1955)] for droplet breakup and we measured the full probability distribution function of droplets radii at different Reynolds numbers and for different volume fractions. By means of a Lagrangian tracking we could follow individual droplets along their trajectories, define a local Weber number based on the velocity gradients, and study its cross-correlation with droplet deformation.
Homogenization and asymptotics for small transaction costs
Soner, H Mete
2012-01-01
We consider the classical Merton problem of lifetime consumption-portfolio optimization problem with small proportional transaction costs. The first order term in the asymptotic expansion is explicitly calculated through a singular ergodic control problem which can be solved in closed form in the one-dimensional case. Unlike the existing literature, we consider a general utility function and general dynamics for the underlying assets. Our arguments are based on ideas from the homogenization theory and use the convergence tools from the theory of viscosity solutions. The multidimensional case is studied in our accompanying paper using the same approach.
Homogeneity Analysis in Xlisp-Stat
Jason Bond
1996-08-01
Full Text Available In this paper a highly interactive, user-friendly Lisp program is introduced to perform homogeneity analysis. A brief introduction to the technique is presented as well as its modification in the presence of missing data. The algorithm and its Lisp implemenation is discussed, and an overview of the object oriented code that produces the interactive dialogs and plots is provided. In order to demonstrate the main features of the program, a small and a large dataset are analyzed. Finally, some comparisons are made with other currently available programs.
Mechanisms in homogeneous and heterogeneous epoxidation catalysis
Oyama, S Ted
2011-01-01
The catalytic epoxidation of olefins plays an important role in the industrial production of several commodity compounds, as well as in the synthesis of many intermediates, fine chemicals, and pharmaceuticals. The scale of production ranges from millions of tons per year to a few grams per year. The diversity of catalysts is large and encompasses all the known categories of catalyst type: homogeneous, heterogeneous, and biological. This book summarizes the current status in these fields concentrating on rates, kinetics, and reaction mechanisms, but also covers broad topics including modeli
Relativistic effects in homogeneous gold catalysis.
Gorin, David J; Toste, F Dean
2007-03-22
Transition-metal catalysts containing gold present new opportunities for chemical synthesis, and it is therefore not surprising that these complexes are beginning to capture the attention of the chemical community. Cationic phosphine-gold(i) complexes are especially versatile and selective catalysts for a growing number of synthetic transformations. The reactivity of these species can be understood in the context of theoretical studies on gold; relativistic effects are especially helpful in rationalizing the reaction manifolds available to gold catalysts. This Review draws on experimental and computational data to present our current understanding of homogeneous gold catalysis, focusing on previously unexplored reactivity and its application to the development of new methodology.
Homogeneous asymmetric catalysis in fragrance chemistry.
Ciappa, Alessandra; Bovo, Sara; Bertoldini, Matteo; Scrivanti, Alberto; Matteoli, Ugo
2008-06-01
Opposite enantiomers of a chiral fragrance may exhibit different olfactory activities making a synthesis in high enantiomeric purity commercially and scientifically interesting. Accordingly, the asymmetric synthesis of four chiral odorants, Fixolide, Phenoxanol, Citralis, and Citralis Nitrile, has been investigated with the aim to develop practically feasible processes. In the devised synthetic schemes, the key step that leads to the formation of the stereogenic center is the homogeneous asymmetric hydrogenation of a prochiral olefin. By an appropriate choice of the catalyst and the reaction conditions, Phenoxanol, Citralis, and Citralis Nitrile were obtained in high enantiomeric purity, and odor profiles of the single enantiomers were determined.
Irregular Homogeneity Domains in Ternary Intermetallic Systems
Jean-Marc Joubert
2015-12-01
Full Text Available Ternary intermetallic A–B–C systems sometimes have unexpected behaviors. The present paper examines situations in which there is a tendency to simultaneously form the compounds ABx, ACx and BCx with the same crystal structure. This causes irregular shapes of the phase homogeneity domains and, from a structural point of view, a complete reversal of site occupancies for the B atom when crossing the homogeneity domain. This work reviews previous studies done in the systems Fe–Nb–Zr, Hf–Mo–Re, Hf–Re–W, Mo–Re–Zr, Re–W–Zr, Cr–Mn–Si, Cr–Mo–Re, and Mo–Ni–Re, and involving the topologically close-packed Laves, χ and σ phases. These systems have been studied using ternary isothermal section determination, DFT calculations, site occupancy measurement using joint X-ray, and neutron diffraction Rietveld refinement. Conclusions are drawn concerning this phenomenon. The paper also reports new experimental or calculated data on Co–Cr–Re and Fe–Nb–Zr systems.
Modified Homogeneous Data Set of Coronal Intensities
Dorotovič, I.; Minarovjech, M.; Lorenc, M.; Rybanský, M.
2014-07-01
The Astronomical Institute of the Slovak Academy of Sciences has published the intensities, recalibrated with respect to a common intensity scale, of the 530.3 nm (Fe xiv) green coronal line observed at ground-based stations up to the year 2008. The name of this publication is Homogeneous Data Set (HDS). We have developed a method that allows one to successfully substitute the ground-based observations by satellite observations and, thus, continue with the publication of the HDS. For this purpose, the observations of the Extreme-ultraviolet Imaging Telescope (EIT), onboard the Solar and Heliospheric Observatory (SOHO) satellite, were exploited. Among other data the EIT instrument provides almost daily 28.4 nm (Fe xv) emission-line snapshots of the corona. The Fe xiv and Fe xv data (4051 observation days) taken in the period 1996 - 2008 have been compared and good agreement was found. The method to obtain the individual data for the HDS follows from the correlation analysis described in this article. The resulting data, now under the name of Modified Homogeneous Data Set (MHDS), are identical up to 1996 to those in the HDS. The MHDS can be used further for studies of the coronal solar activity and its cycle. These data are available at http://www.suh.sk.
The Statistical Mechanics of Ideal Homogeneous Turbulence
Shebalin, John V.
2002-01-01
Plasmas, such as those found in the space environment or in plasma confinement devices, are often modeled as electrically conducting fluids. When fluids and plasmas are energetically stirred, regions of highly nonlinear, chaotic behavior known as turbulence arise. Understanding the fundamental nature of turbulence is a long-standing theoretical challenge. The present work describes a statistical theory concerning a certain class of nonlinear, finite dimensional, dynamical models of turbulence. These models arise when the partial differential equations describing incompressible, ideal (i.e., nondissipative) homogeneous fluid and magnetofluid (i.e., plasma) turbulence are Fourier transformed into a very large set of ordinary differential equations. These equations define a divergenceless flow in a high-dimensional phase space, which allows for the existence of a Liouville theorem, guaranteeing a distribution function based on constants of the motion (integral invariants). The novelty of these particular dynamical systems is that there are integral invariants other than the energy, and that some of these invariants behave like pseudoscalars under two of the discrete symmetry transformations of physics, parity, and charge conjugation. In this work the 'rugged invariants' of ideal homogeneous turbulence are shown to be the only significant scalar and pseudoscalar invariants. The discovery that pseudoscalar invariants cause symmetries of the original equations to be dynamically broken and induce a nonergodic structure on the associated phase space is the primary result presented here. Applicability of this result to dissipative turbulence is also discussed.
Si isotope homogeneity of the solar nebula
Pringle, Emily A.; Savage, Paul S.; Moynier, Frédéric [Department of Earth and Planetary Sciences and McDonnell Center for the Space Sciences, Washington University in St. Louis, One Brookings Drive, St. Louis, MO 63130 (United States); Jackson, Matthew G. [Department of Earth Science, University of California, Santa Barbara, CA 93109 (United States); Barrat, Jean-Alix, E-mail: eapringle@wustl.edu, E-mail: savage@levee.wustl.edu, E-mail: pringle@ipgp.fr, E-mail: moynier@ipgp.fr, E-mail: jackson@geol.ucsb.edu, E-mail: Jean-Alix.Barrat@univ-brest.fr [Université Européenne de Bretagne, Université de Brest, CNRS UMR 6538 (Domaines Océaniques), I.U.E.M., Place Nicolas Copernic, F-29280 Plouzané Cedex (France)
2013-12-20
The presence or absence of variations in the mass-independent abundances of Si isotopes in bulk meteorites provides important clues concerning the evolution of the early solar system. No Si isotopic anomalies have been found within the level of analytical precision of 15 ppm in {sup 29}Si/{sup 28}Si across a wide range of inner solar system materials, including terrestrial basalts, chondrites, and achondrites. A possible exception is the angrites, which may exhibit small excesses of {sup 29}Si. However, the general absence of anomalies suggests that primitive meteorites and differentiated planetesimals formed in a reservoir that was isotopically homogenous with respect to Si. Furthermore, the lack of resolvable anomalies in the calcium-aluminum-rich inclusion measured here suggests that any nucleosynthetic anomalies in Si isotopes were erased through mixing in the solar nebula prior to the formation of refractory solids. The homogeneity exhibited by Si isotopes may have implications for the distribution of Mg isotopes in the solar nebula. Based on supernova nucleosynthetic yield calculations, the expected magnitude of heavy-isotope overabundance is larger for Si than for Mg, suggesting that any potential Mg heterogeneity, if present, exists below the 15 ppm level.
Homogeneous cooling of mixtures of particle shapes
Hidalgo, R. C.; Serero, D.; Pöschel, T.
2016-07-01
In this work, we examine theoretically the cooling dynamics of binary mixtures of spheres and rods. To this end, we introduce a generalized mean field analytical theory, which describes the free cooling behavior of the mixture. The relevant characteristic time scale for the cooling process is derived, depending on the mixture composition and the aspect ratio of the rods. We simulate mixtures of spherocylinders and spheres using a molecular dynamics algorithm implemented on graphics processing unit (GPU) architecture. We systematically study mixtures composed of spheres and rods with several aspect ratios and varying the mixture composition. A homogeneous cooling state, where the time dependence of the system's intensive variables occurs only through a global granular temperature, is identified. We find cooling dynamics in excellent agreement with Haff's law, when using an adequate time scale. Using the scaling properties of the homogeneous cooling dynamics, we estimated numerically the efficiency of the energy interchange between rotational and translational degrees of freedom for collisions between spheres and rods.
Homogenization in micro-magneto-mechanics
Sridhar, A.; Keip, M.-A.; Miehe, C.
2016-07-01
Ferromagnetic materials are characterized by a heterogeneous micro-structure that can be altered by external magnetic and mechanical stimuli. The understanding and the description of the micro-structure evolution is of particular importance for the design and the analysis of smart materials with magneto-mechanical coupling. The macroscopic response of the material results from complex magneto-mechanical interactions occurring on smaller length scales, which are driven by magnetization reorientation and associated magnetic domain wall motions. The aim of this work is to directly base the description of the macroscopic magneto-mechanical material behavior on the micro-magnetic domain evolution. This will be realized by the incorporation of a ferromagnetic phase-field formulation into a macroscopic Boltzmann continuum by the use of computational homogenization. The transition conditions between the two scales are obtained via rigorous exploitation of rate-type and incremental variational principles, which incorporate an extended version of the classical Hill-Mandel macro-homogeneity condition covering the phase field on the micro-scale. An efficient two-scale computational scenario is developed based on an operator splitting scheme that includes a predictor for the magnetization on the micro-scale. Two- and three-dimensional numerical simulations demonstrate the performance of the method. They investigate micro-magnetic domain evolution driven by macroscopic fields as well as the associated overall hysteretic response of ferromagnetic solids.
On Shearing Fluids with Homogeneous Densities
Srivastava, D C; Kumar, Rajesh
2016-01-01
In this paper, we study shearing spherically symmetric homogeneous density fluids in comoving coordinates. It is found that the expansion of the four-velocity of a perfect fluid is homogeneous, whereas its shear is generated by an arbitrary function of time M(t), related to the mass function of the distribution. This function is found to bear a functional relationship with density. The field equations are reduced to two coupled first order ordinary differential equations for the metric coefficients, g 11 and g 22. We have explored a class of solutions assuming that M is a linear function of the density. This class embodies, as a subcase, the complete class of shear-free solutions. We have discussed the off quoted work of Kustaanheimo (1947) and have noted that it deals with shear-free fluids having anisotropic pressure. It is shown that the anisotropy of the fluid is characterized by an arbitrary function of time. We have discussed some issues of historical priorities and credentials related to shear-free sol...
Computational approaches to homogeneous gold catalysis.
Faza, Olalla Nieto; López, Carlos Silva
2015-01-01
Homogenous gold catalysis has been exploding for the last decade at an outstanding pace. The best described reactivity of Au(I) and Au(III) species is based on gold's properties as a soft Lewis acid, but new reactivity patterns have recently emerged which further expand the range of transformations achievable using gold catalysis, with examples of dual gold activation, hydrogenation reactions, or Au(I)/Au(III) catalytic cycles.In this scenario, to develop fully all these new possibilities, the use of computational tools to understand at an atomistic level of detail the complete role of gold as a catalyst is unavoidable. In this work we aim to provide a comprehensive review of the available benchmark works on methodological options to study homogenous gold catalysis in the hope that this effort can help guide the choice of method in future mechanistic studies involving gold complexes. This is relevant because a representative number of current mechanistic studies still use methods which have been reported as inappropriate and dangerously inaccurate for this chemistry.Together with this, we describe a number of recent mechanistic studies where computational chemistry has provided relevant insights into non-conventional reaction paths, unexpected selectivities or novel reactivity, which illustrate the complexity behind gold-mediated organic chemistry.
Real-time assays with molecular beacons and other fluorescent nucleic acid hybridization probes.
Marras, Salvatore A E; Tyagi, Sanjay; Kramer, Fred Russell
2006-01-01
A number of formats for nucleic acid hybridization have been developed to identify DNA and RNA sequences that are involved in cellular processes and that aid in the diagnosis of genetic and infectious diseases. The introduction of hybridization probes with interactive fluorophore pairs has enabled the development of homogeneous hybridization assays for the direct identification of nucleic acids. A change in the fluorescence of these probes indicates the presence of a target nucleic acid, and there is no need to separate unbound probes from hybridized probes. The advantages of homogeneous hybridization assays are their speed and simplicity. In addition, homogeneous assays can be combined with nucleic acid amplification, enabling the detection of rare target nucleic acids. These assays can be followed in real time, providing quantitative determination of target nucleic acids over a broad range of concentrations.
The homogenization of a class of degenerate quasilinear parabolic equations
ZHANG Xingyou; HUANG Yong
2003-01-01
The homogenization of a class of degenerate quasilinear parabolic equations is studied. The Ap weight theory and the classical compensated compactness method are incorporated to obtain the homogenized equation.
MULTISCALE HOMOGENIZATION OF NONLINEAR HYPERBOLIC EQUATIONS WITH SEVERAL TIME SCALES
Jean Louis Woukeng; David Dongo
2011-01-01
We study the multiscale homogenization of a nonlinear hyperbolic equation in a periodic setting. We obtain an accurate homogenization result. We also show that as the nonlinear term depends on the microscopic time variable, the global homogenized problem thus obtained is a system consisting of two hyperbolic equations. It is also shown that in spite of the presence of several time scales, the global homogenized problem is not a reiterated one.
Plasmonics-based SERS nanobiosensor for homogeneous nucleic acid detection.
Wang, Hsin-Neng; Fales, Andrew M; Vo-Dinh, Tuan
2015-05-01
Developing a simple and efficient nucleic acid detection technology is essential for clinical diagnostics. Here, we describe a new conceptually simple and selective "turn on" plasmonics-based nanobiosensor, which integrates non-enzymatic DNA strand-displacement hybridization for specific nucleic acid target identification with surface-enhanced Raman scattering (SERS) detection. This SERS nanobiosensor is a target label-free, and rapid nanoparticle-based biosensing system using a homogeneous assay format that offers a simple and efficient tool for nucleic acid diagnostics. Our results showed that the nanobiosensor provided a limit of detection of ~0.1nM (200amol) in the current bioassay system, and exhibited high specificity for single nucleotide mismatch discrimination. Surface-enhanced Raman scattering (SERS) is a sensitive technique that enhances Raman scattering by molecules adsorbed on rough metal surfaces. The enhancement means that the technique may even detect single molecules. In this article, the authors describe a simple and efficient nucleic acid detection technology using SERS, with "OFF-to-ON" signal switch upon nucleic acid target identification and capture, which provides high sensitivity and specificity for single nucleotide mismatch discrimination. This new technology will be most welcomed in clinical diagnostics. Copyright © 2015 Elsevier Inc. All rights reserved.
Non-homogeneous dynamic Bayesian networks for continuous data
Grzegorczyk, Marco; Husmeier, Dirk
2011-01-01
Classical dynamic Bayesian networks (DBNs) are based on the homogeneous Markov assumption and cannot deal with non-homogeneous temporal processes. Various approaches to relax the homogeneity assumption have recently been proposed. The present paper presents a combination of a Bayesian network with c
THE ANALYTICAL PROPERTIES FOR HOMOGENEOUS RANDOM TRANSITION FUNCTIONS
无
2007-01-01
The concepts of Markov process in random environment and homogeneous random transition functions are introduced. The necessary and sufficient conditions for homogeneous random transition function are given. The main results in this article are the analytical properties, such as continuity, differentiability, random Kolmogorov backward equation and random Kolmogorov forward equation of homogeneous random transition functions.
Against vaccine assay secrecy.
Herder, Matthew; Hatchette, Todd F; Halperin, Scott A; Langley, Joanne M
2015-01-01
Increasing the transparency of the evidence base behind health interventions such as pharmaceuticals, biologics, and medical devices, has become a major point of critique, conflict, and policy focus in recent years. Yet the lack of publicly available information regarding the immunogenicity assays upon which many important, widely used vaccines are based has received no attention to date. In this paper we draw attention to this critical public health problem by reporting on our efforts to secure vaccine assay information in respect of 10 vaccines through Canada's access to information law. We argue, under Canadian law, that the public health interest in having access to the methods for these laboratory procedures should override claims by vaccine manufacturers and regulators that this information is proprietary; and, we call upon several actors to take steps to ensure greater transparency with respect to vaccine assays, including regulators, private firms, researchers, research institutions, research funders, and journal editors.
Herder, Matthew; Hatchette, Todd F; Halperin, Scott A; Langley, Joanne M
2015-01-01
Increasing the transparency of the evidence base behind health interventions such as pharmaceuticals, biologics, and medical devices, has become a major point of critique, conflict, and policy focus in recent years. Yet the lack of publicly available information regarding the immunogenicity assays upon which many important, widely used vaccines are based has received no attention to date. In this paper we draw attention to this critical public health problem by reporting on our efforts to secure vaccine assay information in respect of 10 vaccines through Canada's access to information law. We argue, under Canadian law, that the public health interest in having access to the methods for these laboratory procedures should override claims by vaccine manufacturers and regulators that this information is proprietary; and, we call upon several actors to take steps to ensure greater transparency with respect to vaccine assays, including regulators, private firms, researchers, research institutions, research funders, and journal editors. PMID:25826194
Akers, D.W.; Stoots, C.M.; Kraft, N.C.; Marts, D.J. [Idaho National Engineering Lab., Idaho Falls, ID (United States)
1997-11-01
The Rover Waste Assay System (RWAS) is a nondestructive assay system designed for the rapid assay of highly-enriched {sup 235}U contaminated piping, tank sections, and debris from the Rover nuclear rocket fuel processing facility at the Idaho Chemical Processing Plant. A scanning system translates a NaI(Tl) detector/collimator system over the structural components where both relative and calibrated measurements for {sup 137}Cs are made. Uranium-235 concentrations are in operation and is sufficiently automated that most functions are performed by the computer system. These functions include system calibration, problem identification, collimator control, data analysis, and reporting. Calibration of the system was done through a combination of measurements on calibration standards and benchmarked modeling. A description of the system is presented along with the methods and uncertainties associated with the calibration and analysis of the system for components from the Rover facility. 4 refs., 2 figs., 4 tabs.
Rapid homogenous time-resolved fluorescence (HTRF) immunoassay for anthrax detection.
Cohen, Noam; Mechaly, Adva; Mazor, Ohad; Fisher, Morly; Zahavy, Eran
2014-05-01
Infection with Bacillus anthracsis spores induces an acute anthrax disease that can cause casualties and death in untreated cases. Thus rapid diagnosis of anthrax at early stage of the disease is essential to allow an effective treatment. Here we present the development of rapid and sensitive homogenous time-resolved fluorescence (HTRF) immunoassays based on the energy transfer process of europium cryptate (EuK) donor to AlexaFluor647 acceptor. The energy transfer process is limited to d bacteremia in infected hosts, using two monoclonal anti-PA antibodies that specifically recognize two different epitopes on the PA molecule. The assay was sensitive enabling detection of 2 ng/ml PA in the serum of B. anthracsis-infected rabbits in only 15 min assay. Additionally, HTRF assay was developed for the detection of bacterial spores using polyclonal anti-spore antibodies that recognize many epitopes on the bacterial surface. The assay enabled the detection of 2 × 10(6) spores/ml in 30 min assay and was specific, showing no cross reactivity with closely related non-virulent bacillus cereus strain. This study describes the use of the HTRF assay for the detection of both singled-epitope (proteins) and multi-epitope (particles) as rapid, simple and sensitive method that can be used at the time that fast results are needed to allow an effective medical care.
Ranieri, Elena; Popescu, Iulia; Gigante, Margherita
2014-01-01
Enzyme-linked immune absorbent spot (Elispot) is a quantitative method for measuring relevant parameters of T cell activation. The sensitivity of Elispot allows the detection of low-frequency antigen-specific T cells that secrete cytokines and effector molecules, such as granzyme B and perforin. Cytotoxic T cell (CTL) studies have taken advantage with this high-throughput technology by providing insights into quantity and immune kinetics. Accuracy, sensitivity, reproducibility, and robustness of Elispot resulted in a wide range of applications in research as well as in diagnostic field. Actually, CTL monitoring by Elispot is a gold standard for the evaluation of antigen-specific T cell immunity in clinical trials and vaccine candidates where the ability to detect rare antigen-specific T cells is of relevance for immune diagnostic. The most utilized Elispot assay is the interferon-gamma (IFN-γ) test, a marker for CD8(+) CTL activation, but Elispot can also be used to distinguish different subsets of activated T cells by using other cytokines such as T-helper (Th) 1-type cells (characterized by the production of IFN-γ, IL-2, IL-6, IL-12, IL-21, and TNF-α), Th2 (producing cytokines like IL-4, IL-5, IL-10, and IL-13), and Th17 (IL-17) cells. The reliability of Elispot-generated data, by the evaluation of T cell frequency recognizing individual antigen/peptide, is the core of this method currently applied widely to investigate specific immune responses in cancer, infections, allergies, and autoimmune diseases. The Elispot assay is competing with other methods measuring single-cell cytokine production, e.g., intracellular cytokine by FACS or Miltenyi cytokine secretion assay. Other types of lymphocyte frequency and function assays include limiting dilution assay (LDA), cytotoxic T cell assay (CTL), and tetramer staining. Compared with respect to sensitivity the Elispot assay is outranking other methods to define frequency of antigen-specific lymphocytes. The method
Assays for calcitonin receptors
Teitelbaum, A.P.; Nissenson, R.A.; Arnaud, C.D.
1985-01-01
The assays for calcitonin receptors described focus on their use in the study of the well-established target organs for calcitonin, bone and kidney. The radioligand used in virtually all calcitonin binding studies is /sup 125/I-labelled salmon calcitonin. The lack of methionine residues in this peptide permits the use of chloramine-T for the iodination reaction. Binding assays are described for intact bone, skeletal plasma membranes, renal plasma membranes, and primary kidney cell cultures of rats. Studies on calcitonin metabolism in laboratory animals and regulation of calcitonin receptors are reviewed.
Quantum-dot-based homogeneous time-resolved fluoroimmunoassay of alpha-fetoprotein
Chen Meijun; Wu Yingsong; Lin Guanfeng; Hou Jingyuan; Li Ming [Institute of Antibody Engineering, School of Biotechnology, Southern Medical University, Guangzhou, 510515 (China); Liu Tiancai, E-mail: liutc@smu.edu.cn [Institute of Antibody Engineering, School of Biotechnology, Southern Medical University, Guangzhou, 510515 (China)
2012-09-05
Highlights: Black-Right-Pointing-Pointer QDs-based homogeneous time-resolved fluoroimmunoassay was developed to detect AFP. Black-Right-Pointing-Pointer The conjugates were prepared with QDs-doped microspheres and anti-AFP McAb. Black-Right-Pointing-Pointer The conjugates were prepared with LTCs and another anti-AFP McAb. Black-Right-Pointing-Pointer Excess amounts of conjugates were used for detecting AFP without rinsing. Black-Right-Pointing-Pointer The wedding of QPs and LTCs was suitable for HTRFIA to detect AFP. - Abstract: Quantum dots (QDs) with novel photoproperties are not widely used in clinic diagnosis, and homogeneous time-resolved fluorescence assays possess many advantages over current methods for alpha-fetoprotein (AFP) detection. A novel QD-based homogeneous time-resolved fluorescence assay was developed and used for detection of AFP, a primary marker for many cancers and diseases. QD-doped carboxyl-modified polystyrene microparticles (QPs) were prepared by doping oil-soluble QDs possessing a 605 nm emission peak. The antibody conjugates (QPs-E014) were prepared from QPs and an anti-AFP monoclonal antibody, and luminescent terbium chelates (LTCs) were prepared and conjugated to a second anti-AFP monoclonal antibody (LTCs-E010). In a double-antibodies sandwich structure, QPs-E014 and LTCs-E010 were used for detection of AFP, serving as energy acceptor and donor, respectively, with an AFP bridge. The results demonstrated that the luminescence lifetime of these QPs was sufficiently long for use in a time-resolved fluoroassay, with the efficiency of time-resolved Foerster resonance transfer (TR-FRET) at 67.3% and the spatial distance of the donor to acceptor calculated to be 66.1 Angstrom-Sign . Signals from TR-FRET were found to be proportional to AFP concentrations. The resulting standard curve was log Y = 3.65786 + 0.43863{center_dot}log X (R = 0.996) with Y the QPs fluorescence intensity and X the AFP concentration; the calculated sensitivity was 0
Robardet, E.; Andrieu, S.; Rasmussen, Thomas Bruun
2013-01-01
Twelve National Reference Laboratories (NRLs) for rabies have undertaken a comparative assay to assess the comparison of fluorescent antibody test (FAT) results using five coded commercial anti-rabies conjugates (Biorad, Bioveta, Fujirebio, Millipore, and SIFIN conjugates). Homogenized positive...
Catalysis of Protein Disulfide Bond Isomerization in a Homogeneous Substrate†
Kersteen, Elizabeth A.; Barrows, Seth R.; Raines, Ronald T.
2008-01-01
Protein disulfide isomerase (PDI) catalyzes the rearrangement of nonnative disulfide bonds in the endoplasmic reticulum of eukaryotic cells, a process that often limits the rate at which polypeptide chains fold into a native protein conformation. The mechanism of the reaction catalyzed by PDI is unclear. In assays involving protein substrates, the reaction appears to involve the complete reduction of some or all of its nonnative disulfide bonds followed by oxidation of the resulting dithiols. The substrates in these assays are, however, heterogeneous, which complicates mechanistic analyses. Here, we report the first analysis of disulfide bond isomerization in a homogeneous substrate. Our substrate is based on tachyplesin I, a 17-mer peptide that folds into a _-hairpin stabilized by two disulfide bonds. We describe the chemical synthesis of a variant of tachyplesin I in which its two disulfide bonds are in a nonnative state and side chains near its N-and C-terminus contain a fluorescence donor (tryptophan) and acceptor (N_-dansyllysine). Fluorescence resonance energy transfer from 280 to 465 nm increases by 28-fold upon isomerization of the disulfide bonds into their native state (which has a lower E°_ = -0.313 V than does PDI). We use this continuous assay to analyze catalysis by wild-type human PDI and a variant in which the C-terminal cysteine residue within each Cys—Gly—His—Cys active site is replaced with alanine. We find that wild-type PDI catalyzes the isomerization of the substrate with kcat/KM = 1.7 _ 105 M–1M s–1, which is the largest value yet reported for catalysis of disulfide bond isomerization. The variant, which is a poor catalyst of disulfide bond reduction and dithiol oxidation, retains virtually all of the activity of wild-type PDI in catalysis of disulfide bond isomerization. Thus, the C-terminal cysteine residues play an insignificant role in the isomerization of the disulfide bonds in nonnative tachyplesin I. We conclude that
Catalysis of protein disulfide bond isomerization in a homogeneous substrate.
Kersteen, Elizabeth A; Barrows, Seth R; Raines, Ronald T
2005-09-13
Protein disulfide isomerase (PDI) catalyzes the rearrangement of nonnative disulfide bonds in the endoplasmic reticulum of eukaryotic cells, a process that often limits the rate at which polypeptide chains fold into a native protein conformation. The mechanism of the reaction catalyzed by PDI is unclear. In assays involving protein substrates, the reaction appears to involve the complete reduction of some or all of its nonnative disulfide bonds followed by oxidation of the resulting dithiols. The substrates in these assays are, however, heterogeneous, which complicates mechanistic analyses. Here, we report the first analysis of disulfide bond isomerization in a homogeneous substrate. Our substrate is based on tachyplesin I, a 17-mer peptide that folds into a beta hairpin stabilized by two disulfide bonds. We describe the chemical synthesis of a variant of tachyplesin I in which its two disulfide bonds are in a nonnative state and side chains near its N and C terminus contain a fluorescence donor (tryptophan) and acceptor (N(epsilon)-dansyllysine). Fluorescence resonance energy transfer from 280 to 465 nm increases by 28-fold upon isomerization of the disulfide bonds into their native state (which has a lower E(o') = -0.313 V than does PDI). We use this continuous assay to analyze catalysis by wild-type human PDI and a variant in which the C-terminal cysteine residue within each Cys-Gly-His-Cys active site is replaced with alanine. We find that wild-type PDI catalyzes the isomerization of the substrate with kcat/K(M) = 1.7 x 10(5) M(-1) s(-1), which is the largest value yet reported for catalysis of disulfide bond isomerization. The variant, which is a poor catalyst of disulfide bond reduction and dithiol oxidation, retains virtually all of the activity of wild-type PDI in catalysis of disulfide bond isomerization. Thus, the C-terminal cysteine residues play an insignificant role in the isomerization of the disulfide bonds in nonnative tachyplesin I. We conclude
Correlators of Matrix Models on Homogeneous Spaces
Kitazawa, Y; Tomino, D; Kitazawa, Yoshihisa; Takayama, Yastoshi; Tomino, Dan
2004-01-01
We investigate the correlators of TrA_{mu}A_{nu} in matrix models on homogeneous spaces: S^2 and S^2 x S^2. Their expectation value is a good order parameter to measure the geometry of the space on which non-commutative gauge theory is realized. They also serve as the Wilson lines which carry the minimum momentum. We develop an efficient procedure to calculate them through 1PI diagrams. We determine the large N scaling behavior of the correlators. The order parameter shows that fuzzy S^2 x S^2 acquires a 4 dimensional fractal structure in contrast to fuzzy S^2. We also find that the two point functions exhibit logarithmic scaling violations.
On the Persistence of Homogeneous Matter
Butterfield, J
2004-01-01
Some recent philosophical debate about persistence has focussed on an argument against perdurantism that discusses rotating perfectly homogeneous discs (the `rotating discs argument'; RDA). The argument has been mostly discussed by metaphysicians, though it appeals to ideas from classical mechanics, especially about rotation. In contrast, I assess the RDA from the perspective of the philosophy of physics. After introducing the argument and emphasizing the relevance of physics (Sections 1 to 3), I review some metaphysicians' replies to the argument (Section 4). Thereafter, I argue for three main conclusions. They all arise from the fact, emphasized in Section 2, that classical mechanics (non-relativistic as well as relativistic) is both more subtle, and more problematic, than philosophers generally realize. The main conclusion is that the RDA can be defeated (Section 6 onwards). Namely, by the perdurantist taking objects in classical mechanics (whether point-particles or continuous bodies) to have only tempora...
Homogeneous spacelike singularities inside spherical black holes
Burko, L M
1997-01-01
Recent numerical simulations have found that the Cauchy horizon inside spherical charged black holes, when perturbed nonlinearly by a self-gravitating, minimally-coupled, massless, spherically-symmetric scalar field, turns into a null weak singularity which focuses monotonically to $r=0$ at late times, where the singularity becomes spacelike. Our main objective is to study this spacelike singularity. We study analytically the spherically-symmetric Einstein-Maxwell-scalar equations asymptotically near the singularity. We obtain a series-expansion solution for the metric functions and for the scalar field near $r=0$ under the simplifying assumption of homogeneity. Namely, we neglect spatial derivatives and keep only temporal derivatives. We find that there indeed exists a generic spacelike singularity solution for these equations (in the sense that the solution depends on enough free parameters), with similar properties to those found in the numerical simulations. This singularity is strong in the Tipler sense,...
Modeling of nanoplastic by asymptotic homogenization method
张为民; 何伟; 李亚; 张平; 张淳源
2008-01-01
The so-called nanoplastic is a new simple name for the polymer/layered silicate nanocomposite,which possesses excellent properties.The asymptotic homogenization method(AHM) was applied to determine numerically the effective elastic modulus of a two-phase nanoplastic with different particle aspect ratios,different ratios of elastic modulus of the effective particle to that of the matrix and different volume fractions.A simple representative volume element was proposed,which is assumed that the effective particles are uniform well-aligned and perfectly bonded in an isotropic matrix and have periodic structure.Some different theoretical models and the experimental results were compared.The numerical results are good in agreement with the experimental results.
Leith diffusion model for homogeneous anisotropic turbulence
Rubinstein, Robert; Clark, Timothy; Kurien, Susan
2016-11-01
A new spectral closure model for homogeneous anisotropic turbulence is proposed. The systematic development begins by closing the third-order correlation describing nonlinear interactions by an anisotropic generalization of the Leith diffusion model for isotropic turbulence. The correlation tensor is then decomposed into a tensorially isotropic part, or directional anisotropy, and a trace-free remainder, or polarization anisotropy. The directional and polarization components are then decomposed using irreducible representations of the SO(3) symmetry group. Under the ansatz that the decomposition is truncated at quadratic order, evolution equations are derived for the directional and polarization pieces of the correlation tensor. Numerical simulation of the model equations for a freely decaying anisotropic flow illustrate the non-trivial effects of spectral dependencies on the different return-to-isotropy rates of the directional and polarization contributions.
Homogeneous cosmology with aggressively expanding civilizations
Olson, S Jay
2014-01-01
In the context of a homogeneous universe, we note that the appearance of aggressively expanding advanced life is geometrically similar to the process of nucleation and bubble growth in a first-order cosmological phase transition. We exploit this similarity to describe the dynamics of life saturating the universe on a cosmic scale, adapting the phase transition model to incorporate probability distributions of expansion and resource consumption strategies. Through a series of numerical solutions covering several orders of magnitude in the input assumption parameters, the resulting cosmological model is used to address basic questions related to the intergalactic spreading of life, dealing with issues such as timescales, observability, competition between strategies, and first-mover advantage. Finally, we examine physical effects on the universe itself, such as reheating and the backreaction on the evolution of the scale factor, if such life is able to control and convert a significant fraction of the available...
Inertial particles in homogeneous shear turbulence
Nicolai, Claudia; Jacob, Boris [CNR-INSEAN, via di Vallerano 139, 00128 Rome (Italy); Gualtieri, Paolo; Piva, Renzo, E-mail: claudia.nicolai@uniroma1.it [DMA, Sapienza Universita di Roma, Via Eudossiana 18, 00184 Rome (Italy)
2011-12-22
The characteristics of inertial particles distribution in a uniformly sheared turbulent flow are investigated, with the aim of quantifying the effects associated with the large-scale anisotropy induced by the mean velocity gradient. The focus of the analysis is on clustering aspects, and in particular on the dependence of the radial distribution function on both the directionality and the magnitude of the observation scale. We discuss experimental data measured in a homogeneous shear flow seeded with particles of size comparable with the Kolmogorov length scale and Stokes number St Almost-Equal-To 0.3, and discuss their distribution properties in comparison with results provided by related one-way coupled direct numerical simulations which make use of the point-force approximation.
Autophoretic self-propulsion of homogeneous particles
Michelin, Sebastien; Lauga, Eric; de Canio, Gabriele
2014-11-01
Phoretic mechanisms such as diffusiophoresis exploit short-ranged interactions between solute molecules in the fluid and a rigid wall to generate local slip velocities in the presence of solute gradients along the solid boundary. This boundary flow can result in macroscopic fluid motion or phoretic migration of inert particles. These mechanisms have recently received a renewed interest to design self-propelled ``autophoretic'' systems able to generate the required solute gradients through chemical reaction at their surface. Most existing designs rely on the asymmetric chemical treatment of the particle's surface to guarantee symmetry-breaking and the generation of a net flow. We show here, however, that chemical asymmetry is not necessary for flow generation and that homogeneous particles with asymmetric geometry may lead to self-propulsion in Stokes flow. Similarly, this principle can be used to manufacture micro-pumps using channel walls with uniform chemical properties.
Energy interactions in homogeneously sheared magnetohydrodynamic flows
Collard, Diane; Praturi, Divya Sri; Girimaji, Sharath
2016-11-01
We investigate the behavior of homogeneously sheared magnetohydrodynamic (MHD) flows subject to perturbations in various directions. We perform rapid distortion theory (RDT) analysis and direct numerical simulations (DNS) to examine the interplay between magnetic, kinetic, and internal energies. For perturbation wavevectors oriented along the spanwise direction, RDT analysis shows that the magnetic and velocity fields are decoupled. In the case of streamwise wavevectors, the magnetic and velocity fields are tightly coupled. The coupling is "harmonic" in nature. DNS is then used to confirm the RDT findings. Computations of spanwise perturbations indeed exhibit behavior that is impervious to the magnetic field. Computed streamwise perturbations exhibit oscillatory evolution of kinetic and magnetic energies for low magnetic field strength. As the strength of magnetic field increases, the oscillatory behavior intensifies even as the energy magnitude decays, indicating strong stabilization.
Temperature field simulation of laser homogenizing equipment
Juanjuan WANG; Yunshan WANG; Fudong ZHU
2009-01-01
The laser homogenizing equipment was devised using the ring scanning principle. Its working principle is explained. A laser scanning ring facula is obtained when the laser beam goes through the equipment's optical system rotating with high-frequency. The scanning ring facula's mathematic model is established based on the temperature field's superposing principle. The ring facula's light intensity distribution and temperature distribution characteristics are achieved by simulating its temperature field. By studying the effect of parameters on the temperature field, the best parameters can be found. Results show that favorable temperature distribution characteristics can be attained by choosing appropriate parameters, and even the thermal effect can be realized by utilizing the circumference power compensating for the heat exchange lost in the horizontal direction. The uniform hardness layer and better process quality can be attained using the ring facula optimized for metal laser heat treatment.
Homogenization analysis of complementary waveguide metamaterials
Landy, Nathan; Hunt, John; Smith, David R.
2013-11-01
We analyze the properties of complementary metamaterials as effective inclusions patterned into the conducting walls of metal waveguide structures. We show that guided wave metamaterials can be homogenized using the same retrieval techniques used for volumetric metamaterials, leading to a description in which a given complementary element is conceptually replaced by a block of material within the waveguide whose effective permittivity and permeability result in equivalent scattering characteristics. The use of effective constitutive parameters for waveguide materials provides an alternative point-of-view for the design of waveguide and microstrip based components, including planar lenses and filters, as well as devices with derived from a bulk material response. In addition to imparting effective constitutive properties to the waveguide, complementary metamaterials also couple energy from waveguide modes into radiation. Thus, complementary waveguide metamaterials can be used to modify and optimize a variety of antenna structures.
The homogeneity conjecture for supergravity backgrounds
Figueroa-O'Farrill, José Miguel
2009-06-01
These notes record three lectures given at the workshop "Higher symmetries in Physics", held at the Universidad Complutense de Madrid in November 2008. In them we explain how to construct a Lie (super)algebra associated to a spin manifold, perhaps with extra geometric data, and a notion of privileged spinors. The typical examples are supersymmetric supergravity backgrounds; although there are more classical instances of this construction. We focus on two results: the geometric constructions of compact real forms of the simple Lie algebras of type B4, F4 and E8 from S7, S8 and S15, respectively; and the construction of the Killing superalgebra of eleven-dimensional supergravity backgrounds. As an application of this latter construction we show that supersymmetric supergravity backgrounds with enough supersymmetry are necessarily locally homogeneous.
Homogenization of global radiosonde humidity data
Blaschek, Michael; Haimberger, Leopold
2016-04-01
The global radiosonde network is an important source of upper-air measurements and is strongly connected to reanalysis efforts of the 20th century. However, measurements are strongly affected by changes in the observing system and require a homogenization before they can be considered useful in climate studies. In particular humidity measurements are known to show spurious trends and biases induced by many sources, e.g. reporting practices or freezing of the sensor. We propose to detect and correct these biases in an automated way, as has been done with temperature and winds. We detect breakpoints in dew point depression (DPD) time series by employing a standard normal homogeneity test (SNHT) on DPD-departures from ERA-Interim. In a next step, we calculate quantile departures between the latter and the earlier part near the breakpoints of the time series, going back in time. These departures adjust the earlier distribution of DPD to the latter distribution, called quantile matching, thus removing for example a non climatic shift. We employ this approach to the existing radiosonde network. In a first step to verify our approach we compare our results with ERA-Interim data and brightness temperatures of humidity-sensitive channels of microwave measuring radiometers (SSMIS) onboard DMSP F16. The results show that some of the biases can be detected and corrected in an automated way, however large biases that impact the distribution of DPD values originating from known reporting practices (e.g. 30 DPD on US stations) remain. These biases can be removed but not corrected. The comparison of brightness temperatures from satellite and radiosondes proofs to be difficult as large differences result from for example representative errors.
Harndahl, Mikkel; Rasmussen, Michael; Røder, Gustav Andreas;
2011-01-01
timed data needed to determine the rate of dissociation is not simple. Ideally, one should use a homogenous assay involving an inexhaustible and label-free assay principle. Here, we present a homogenous, high-throughput peptide-MHC class I dissociation assay, which by and large fulfill these ideal...... requirements. To avoid labeling of the highly variable peptide, we labeled the invariant ß2m and monitored its dissociation by a scintillation proximity assay, which has no separation steps and allows for real-time quantitative measurement of dissociation. Validating this work-around to create a virtually...
Is it possible to homogenize resonant chiral metamaterials ?
Andryieuski, Andrei; Menzel, Christoph; Rockstuhl, Carsten
2010-01-01
Homogenization of metamaterials is very important as it makes possible description in terms of effective parameters. In this contribution we consider the homogenization of chiral metamaterials. We show that for some metamaterials there is an optimal meta-atom size which depends on the coupling...... between meta-atoms. We introduce numerical criterion of homogeneity on the basis of the Bloch modes dispersion diagram calculation and a tool to predict the homogeneity limit. We show that some metamaterials with strong coupling between meta-atoms cannot be considered as homogeneous at all...
New oligosaccharyltransferase assay method.
Kohda, Daisuke; Yamada, Masaki; Igura, Mayumi; Kamishikiryo, Jun; Maenaka, Katsumi
2007-11-01
We developed a new in vitro assay for oligosaccharyltransferase (OST), which catalyzes the transfer of preassembled oligosaccharides on lipid carriers onto asparagine residues in polypeptide chains. The asparagine residues reside in the sequon, Asn-X-Thr/Ser, where X can be any amino acid residue except Pro. We demonstrate the potency of our assay using the OST from yeast. In our method, polyacrylamide gel electrophoresis is used to separate the glycopeptide products from the peptide substrates. The substrate peptide is fluorescently labeled and the formation of glycopeptides is analyzed by fluorescence gel imaging. Two in vitro OST assay methods are now widely used, but both the methods depend on previous knowledge of the oligosaccharide moiety: One method uses lectin binding as the separation mechanism and the other method uses biosynthetically or chemoenzymatically synthesized lipid-linked oligosaccharides as donors. N-linked protein glycosylation is found in all three domains of life, but little is known about the N-glycosylation in Archaea. Thus, our new assay, which does not require a priori knowledge of the oligosaccharides, will be useful in such cases. Indeed, we have detected the OST activity in the membrane fraction from a hyperthermophilic archaeon, Pyrococcus furiosus.
Itenov, Theis S; Kirkby, Nikolai S; Bestle, Morten H
2015-01-01
BACKGROUD: Hyaluronic acid (HA) is proposed as a marker of functional liver capacity. The aim of the present study was to compare a new turbidimetric assay for measuring HA with the current standard method. METHODS: HA was measured by a particle-enhanced turbidimetric immunoassay (PETIA) and enzyme...
Instrument for assaying radiation
Coleman, Jody Rustyn; Farfan, Eduardo B.
2016-03-22
An instrument for assaying radiation includes a flat panel detector having a first side opposed to a second side. A collimated aperture covers at least a portion of the first side of the flat panel detector. At least one of a display screen or a radiation shield may cover at least a portion of the second side of the flat panel detector.
On the decay of homogeneous isotropic turbulence
Skrbek, L.; Stalp, Steven R.
2000-08-01
Decaying homogeneous, isotropic turbulence is investigated using a phenomenological model based on the three-dimensional turbulent energy spectra. We generalize the approach first used by Comte-Bellot and Corrsin [J. Fluid Mech. 25, 657 (1966)] and revised by Saffman [J. Fluid Mech. 27, 581 (1967); Phys. Fluids 10, 1349 (1967)]. At small wave numbers we assume the spectral energy is proportional to the wave number to an arbitrary power. The specific case of power 2, which follows from the Saffman invariant, is discussed in detail and is later shown to best describe experimental data. For the spectral energy density in the inertial range we apply both the Kolmogorov -5/3 law, E(k)=Cɛ2/3k-5/3, and the refined Kolmogorov law by taking into account intermittency. We show that intermittency affects the energy decay mainly by shifting the position of the virtual origin rather than altering the power law of the energy decay. Additionally, the spectrum is naturally truncated due to the size of the wind tunnel test section, as eddies larger than the physical size of the system cannot exist. We discuss effects associated with the energy-containing length scale saturating at the size of the test section and predict a change in the power law decay of both energy and vorticity. To incorporate viscous corrections to the model, we truncate the spectrum at an effective Kolmogorov wave number kη=γ(ɛ/v3)1/4, where γ is a dimensionless parameter of order unity. We show that as the turbulence decays, viscous corrections gradually become more important and a simple power law can no longer describe the decay. We discuss the final period of decay within the framework of our model, and show that care must be taken to distinguish between the final period of decay and the change of the character of decay due to the saturation of the energy containing length scale. The model is applied to a number of experiments on decaying turbulence. These include the downstream decay of turbulence in
Homogeneous Detection of Human IgG by Gold Nanoparticle Probes
CAO Qihua; YUAN Hong; CAI Ruxiu
2009-01-01
A simple,and homogeneous detection system for human IgG based on the optical properties of aggregated gold nanoparticles probes were investigated.When gold nanoparticles with about 13 nm in diameter were modified by goat anti-human IgG,the addition of human IgG could change the absorption of colloidal gold solution,and the absorption intensity at 740 nm depended on the amount of human IgG.The aggregation of gold nanoparticles was also validated using transmission electron microscopy(TEM).A series of experiments were carried out to study the effects of pH value, the reaction temperature,and non-specific adsorption on the assay.A dynamic range of 10-500μg/~3 mL human IgG was observed.The new bioassay could be used for the rapid and ho-mogeneous detection of antibodies in bioanalytical chemistry.
Ziche, Marina; Morbidelli, Lucia
2015-01-01
The cornea in most species is physiologically avascular, and thus this assay allows the measurement of newly formed vessels. The continuous monitoring of neovascular growth in the same animal allows the evaluation of drugs acting as suppressors or stimulators of angiogenesis. Under anesthesia a micropocket is produced in the cornea thickness and the angiogenesis stimulus (tumor tissue, cell suspension, growth factor) is placed into the pocket in order to induce vascular outgrowth from the limbal capillaries. Neovascular development and progression can be modified by the presence of locally released or applied inhibitory factors or by systemic treatments. In this chapter the experimental details of the avascular cornea assay, the technical challenges, and advantages and disadvantages in different species are discussed. Protocols for local drug treatment and tissue sampling for histology and pharmacokinetic profile are reported.
Kinetic Tetrazolium Microtiter Assay
Pierson, Duane L.; Stowe, Raymond; Koenig, David
1993-01-01
Kinetic tetrazolium microtiter assay (KTMA) involves use of tetrazolium salts and Triton X-100 (or equivalent), nontoxic, in vitro color developer solubilizing colored metabolite formazan without injuring or killing metabolizing cells. Provides for continuous measurement of metabolism and makes possible to determine rate of action of antimicrobial agent in real time as well as determines effective inhibitory concentrations. Used to monitor growth after addition of stimulatory compounds. Provides for kinetic determination of efficacy of biocide, greatly increasing reliability and precision of results. Also used to determine relative effectiveness of antimicrobial agent as function of time. Capability of generating results on day of test extremely important in treatment of water and waste, disinfection of hospital rooms, and in pharmaceutical, agricultural, and food-processing industries. Assay also used in many aspects of cell biology.
Numerical computation of homogeneous slope stability.
Xiao, Shuangshuang; Li, Kemin; Ding, Xiaohua; Liu, Tong
2015-01-01
To simplify the computational process of homogeneous slope stability, improve computational accuracy, and find multiple potential slip surfaces of a complex geometric slope, this study utilized the limit equilibrium method to derive expression equations of overall and partial factors of safety. This study transformed the solution of the minimum factor of safety (FOS) to solving of a constrained nonlinear programming problem and applied an exhaustive method (EM) and particle swarm optimization algorithm (PSO) to this problem. In simple slope examples, the computational results using an EM and PSO were close to those obtained using other methods. Compared to the EM, the PSO had a small computation error and a significantly shorter computation time. As a result, the PSO could precisely calculate the slope FOS with high efficiency. The example of the multistage slope analysis indicated that this slope had two potential slip surfaces. The factors of safety were 1.1182 and 1.1560, respectively. The differences between these and the minimum FOS (1.0759) were small, but the positions of the slip surfaces were completely different than the critical slip surface (CSS).
Convective mixing in homogeneous porous media flow
Ching, Jia-Hau; Chen, Peilong; Tsai, Peichun Amy
2017-01-01
Inspired by the flow processes in the technology of carbon dioxide (CO2) storage in saline formations, we modeled a homogeneous porous media flow in a Hele-Shaw cell to investigate density-driven convection due to dissolution. We used an analogy of the fluid system to mimic the diffusion and subsequent convection when CO2 dissolves in brine, which generates a heavier solution. By varying the permeability, we examined the onset of convection, the falling dynamics, the wavelengths of fingers, and the rate of dissolution, for the Rayleigh number Ra (a dimensionless forcing term which is the ratio of buoyancy to diffusivity) in the range of 2.0 ×104≤Ra≤8.26 ×105 . Our results reveal that the effect of permeability influences significantly the initial convective speed, as well as the later coarsening dynamics of the heavier fingering plumes. However, the total dissolved mass, characterized by a nondimensional Nusselt number Nu, has an insignificant dependence on Ra. This implies that the total dissolution rate of CO2 is nearly constant in high Ra geological porous structures.
Chemically homogeneous evolution in massive binaries
de Mink, S E; Langer, N; Pols, O R
2010-01-01
Rotation can have severe consequences for the evolution of massive stars. It is now considered as one of the main parameters, alongside mass and metallicity that determine the final fate of single stars. In massive, fast rotating stars mixing processes induced by rotation may be so efficient that helium produced in the center is mixed throughout the envelope. Such stars evolve almost chemically homogeneously. At low metallicity they remain blue and compact, while they gradually evolve into Wolf-Rayet stars and possibly into progenitors of long gamma-ray bursts. In binaries this type of evolution may occur because of (I) tides in very close binaries, as a result of (II) spin up by mass transfer, as result of (III) a merger of the two stars and (IV) when one of the components in the binary was born with a very high initial rotation rate. As these stars stay compact, the evolutionary channels are very different from what classical binary evolutionary models predict. In this contribution we discuss examples of ne...
Development of a mechanical homogenizer coffee
Raphael Magalhães Gomes Moreira
2013-12-01
Full Text Available The crop Coffee demands investments to the machines development, which it enables the processes ofpost-harvesting, becoming them faster, meanwhile improving the working. The use of stationary layer dryers are responsible for good results at drying and product quality, but it requires a constant revolving, in order to homogenize the grain mass and improve the air distribution inside the drying chamber. The shortage of workmanship, associated to the need of constant revolving and the heavy working conditions, it becomes it indispensable to mechanize, in some way, this step. The agricultural machine design is considered of great complexity, regarding it must be concerned with the interactions among the operator, machine and environment. When designing a machine, the experience and the dominion of several standpoints have to be interpreted clearly. With the increasing competitiveness on the consumer market and agricultural machines, several companies have joined the research centers, because in general, do not make use of systematic procedures during the project, which can result in failures during operation. This article aimed to design and build a semi-mechanized revolving prototype used to mix the coffee beans. The revolving prototype with the helical screw principles proved to be able to performing the grains transport efficiently.
Numerical Computation of Homogeneous Slope Stability
Shuangshuang Xiao
2015-01-01
Full Text Available To simplify the computational process of homogeneous slope stability, improve computational accuracy, and find multiple potential slip surfaces of a complex geometric slope, this study utilized the limit equilibrium method to derive expression equations of overall and partial factors of safety. This study transformed the solution of the minimum factor of safety (FOS to solving of a constrained nonlinear programming problem and applied an exhaustive method (EM and particle swarm optimization algorithm (PSO to this problem. In simple slope examples, the computational results using an EM and PSO were close to those obtained using other methods. Compared to the EM, the PSO had a small computation error and a significantly shorter computation time. As a result, the PSO could precisely calculate the slope FOS with high efficiency. The example of the multistage slope analysis indicated that this slope had two potential slip surfaces. The factors of safety were 1.1182 and 1.1560, respectively. The differences between these and the minimum FOS (1.0759 were small, but the positions of the slip surfaces were completely different than the critical slip surface (CSS.
Dynamic contact angle cycling homogenizes heterogeneous surfaces.
Belibel, R; Barbaud, C; Mora, L
2016-12-01
In order to reduce restenosis, the necessity to develop the appropriate coating material of metallic stent is a challenge for biomedicine and scientific research over the past decade. Therefore, biodegradable copolymers of poly((R,S)-3,3 dimethylmalic acid) (PDMMLA) were prepared in order to develop a new coating exhibiting different custom groups in its side chain and being able to carry a drug. This material will be in direct contact with cells and blood. It consists of carboxylic acid and hexylic groups used for hydrophilic and hydrophobic character, respectively. The study of this material wettability and dynamic surface properties is of importance due to the influence of the chemistry and the potential motility of these chemical groups on cell adhesion and polymer kinetic hydrolysis. Cassie theory was used for the theoretical correction of contact angles of these chemical heterogeneous surfaces coatings. Dynamic Surface Analysis was used as practical homogenizer of chemical heterogeneous surfaces by cycling during many cycles in water. In this work, we confirmed that, unlike receding contact angle, advancing contact angle is influenced by the difference of only 10% of acidic groups (%A) in side-chain of polymers. It linearly decreases with increasing acidity percentage. Hysteresis (H) is also a sensitive parameter which is discussed in this paper. Finally, we conclude that cycling provides real information, thus avoiding theoretical Cassie correction. H(10)is the most sensible parameter to %A.
Theoretical Studies of Homogeneous Catalysts Mimicking Nitrogenase
Alessandra Magistrato
2011-01-01
Full Text Available The conversion of molecular nitrogen to ammonia is a key biological and chemical process and represents one of the most challenging topics in chemistry and biology. In Nature the Mo-containing nitrogenase enzymes perform nitrogen ‘fixation’ via an iron molybdenum cofactor (FeMo-co under ambient conditions. In contrast, industrially, the Haber-Bosch process reduces molecular nitrogen and hydrogen to ammonia with a heterogeneous iron catalyst under drastic conditions of temperature and pressure. This process accounts for the production of millions of tons of nitrogen compounds used for agricultural and industrial purposes, but the high temperature and pressure required result in a large energy loss, leading to several economic and environmental issues. During the last 40 years many attempts have been made to synthesize simple homogeneous catalysts that can activate dinitrogen under the same mild conditions of the nitrogenase enzymes. Several compounds, almost all containing transition metals, have been shown to bind and activate N2 to various degrees. However, to date Mo(N2(HIPTN3N with (HIPTN3N= hexaisopropyl-terphenyl-triamidoamine is the only compound performing this process catalytically. In this review we describe how Density Functional Theory calculations have been of help in elucidating the reaction mechanisms of the inorganic compounds that activate or fix N2. These studies provided important insights that rationalize and complement the experimental findings about the reaction mechanisms of known catalysts, predicting the reactivity of new potential catalysts and helping in tailoring new efficient catalytic compounds.
Homogenizing atomic dynamics by fractional differential equations
Tang, Shaoqiang; Ying, Yuping
2017-10-01
In this paper, we propose two ways to construct fractional differential equations (FDE) for approximating atomic chain dynamics. Taking harmonic chain as an example, we add a power function of fractional order to Taylor expansion of the dispersion relation, and determine the parameters by matching two selected wave numbers. This approximate function leads to an FDE after considering both directions for wave propagation. As an alternative, we consider the symbol of the force term, and approximate it by a similar function. It also induces an FDE. Both approaches produce excellent agreement with the harmonic chain dynamics. The accuracy may be improved by optimizing the selected wave numbers, or starting with higher order Taylor expansions. When resolved in the lattice constant, the resulting FDE's faithfully reproduce the lattice dynamics. When resolved in a coarse grid instead, they systematically generate homogenized algorithms. Numerical tests are performed to verify the proposed approaches. Moreover, FDE's are also constructed for diatomic chain and anharmonic lattice, to illustrate the generality of the proposed approaches.
On the Homogeneous Model Of Euclidean Geometry
Gunn, Charles
2011-01-01
We attach the degenerate signature (n,0,1) to the projectivized dual Grassmann algebra over R(n+1). We explore the use of the resulting Clifford algebra as a model for euclidean geometry. We avoid problems with the degenerate metric by constructing an algebra isomorphism between this Grassmann algebra and its dual, that yields non-metric meet and join operators. We review the Cayley-Klein construction of the projective (homogeneous) model for euclidean geometry leading to the choice of the signature (n,0,1). We focus on the cases of n=2 and n=3 in detail, enumerating the geometric products between simple k- and m-vectors. We establish that versor (sandwich) operators provide all euclidean isometries, both direct and indirect. We locate the spin group, a double cover of the direct euclidean group, inside the even subalgebra of the Clifford algebra, and provide a simple algorithm for calculating the logarithm of such elements. We conclude with an elementary account of euclidean rigid body motion within this fra...
Linear perturbations of spatially locally homogeneous spacetimes
Tanimoto, M
2003-01-01
Methods and properties regarding the linear perturbations are discussed for some spatially closed (vacuum) solutions of Einstein's equation. The main focus is on two kinds of spatially locally homogeneous solution; one is the Bianchi III (Thurston's H^2 x R) type, while the other is the Bianchi II (Thurston's Nil) type. With a brief summary of previous results on the Bianchi III perturbations, asymptotic solutions for the gauge-invariant variables for the Bianchi III are shown, with which (in)stability of the background solution is also examined. The issue of linear stability for a Bianchi II solution is still an open problem. To approach it, appropriate eigenfunctions are presented for an explicitly compactified Bianchi II manifold and based on that, some field equations on the Bianchi II background spacetime are studied. Differences between perturbation analyses for Bianchi class B (to which Bianchi III belongs) and class A (to which Bianchi II belongs) are stressed for an intention to be helpful for applic...
Magnetic field homogeneity for neutron EDM experiment
Anderson, Melissa
2016-09-01
The neutron electric dipole moment (nEDM) is an observable which, if non-zero, would violate time-reversal symmetry, and thereby charge-parity symmetry of nature. New sources of CP violation beyond those found in the standard model of particle physics are already tightly constrained by nEDM measurements. Our future nEDM experiment seeks to improve the precision on the nEDM by a factor of 30, using a new ultracold neutron (UCN) source that is being constructed at TRIUMF. Systematic errors in the nEDM experiment are driven by magnetic field inhomogeneity and instability. The goal field inhomogeneity averaged over the experimental measurement cell (order of 1 m) is 1 nT/m, at a total magnetic field of 1 microTesla. This equates to roughly 10-3 homogeneity. A particularly challenging aspect of the design problem is that nearby magnetic materials will also affect the magnetic inhomogeneity, and this must be taken into account in completing the design. This poster will present the design methodology and status of the main coil for the experiment where we use FEA software (COMSOL) to simulate and analyze the magnetic field. Natural Sciences and Engineering Research Council.
Abrignani, Sergio; Tonti, Elena; Casorati, Giulia; Dellabona, Paolo
2009-01-01
Activation, proliferation and differentiation of naïve B lymphocytes into memory B cells and plasma cells requires engagement of the B cell receptor (BCR) coupled to T-cell help (1, 2). T cells deliver help in cognate fashion when they are activated upon recognition of specific MHC-peptide complexes presented by B cells. T cells can also deliver help in a non-cognate or bystander fashion, when they do not find specific MHC-peptide complexes on B cells and are activated by alternative mechanisms. T-cell dependent activation of B cells can be studied in vitro by experimental models called "B cell helper assays" that are based on the co-culture of B cells with activated T cells. These assays allow to decipher the molecular bases for productive T-dependent B cell responses. We show here examples of B cell helper assays in vitro, which can be reproduced with any subset of T lymphocytes that displays the appropriate helper signals.
Variation principle of piezothermoelastic bodies, canonical equation and homogeneous equation
LIU Yan-hong; ZHANG Hui-ming
2007-01-01
Combining the symplectic variations theory, the homogeneous control equation and isoparametric element homogeneous formulations for piezothermoelastic hybrid laminates problems were deduced. Firstly, based on the generalized Hamilton variation principle, the non-homogeneous Hamilton canonical equation for piezothermoelastic bodies was derived. Then the symplectic relationship of variations in the thermal equilibrium formulations and gradient equations was considered, and the non-homogeneous canonical equation was transformed to homogeneous control equation for solving independently the coupling problem of piezothermoelastic bodies by the incensement of dimensions of the canonical equation. For the convenience of deriving Hamilton isoparametric element formulations with four nodes, one can consider the temperature gradient equation as constitutive relation and reconstruct new variation principle. The homogeneous equation simplifies greatly the solution programs which are often performed to solve nonhomogeneous equation and second order differential equation on the thermal equilibrium and gradient relationship.
Stochastic homogenization of rate-independent systems and applications
Heida, Martin
2017-05-01
We study the stochastic and periodic homogenization 1-homogeneous convex functionals. We prove some convergence results with respect to stochastic two-scale convergence, which are related to classical Γ -convergence results. The main result is a general \\liminf -estimate for a sequence of 1-homogeneous functionals and a two-scale stability result for sequences of convex sets. We apply our results to the homogenization of rate-independent systems with 1-homogeneous dissipation potentials and quadratic energies. In these applications, both the energy and the dissipation potential have an underlying stochastic microscopic structure. We study the particular homogenization problems of Prandtl-Reuss plasticity, Tresca friction on a macroscopic surface and Tresca friction on microscopic fissures.
Universal fieldable assay with unassisted visual detection
Chelyapov, Nicolas (Inventor)
2012-01-01
A universal detection system based on allosteric aptamers, signal amplification cascade, and eye-detectable phrase transition. A broadly applicable homogeneous detection system is provided. It utilizes components of the blood coagulation cascade in the presence of polystyrene microspheres (MS) as a signal amplifier. Russell's viper venom factor X activator (RVV-X) triggers the cascade, which results in an eye-visible phase transition--precipitation of MS bound to clotted fibrin. An allosteric RNA aptamer, RNA132, with affinity for RVV-X and human vascular endothelial growth factor (VEGF.sub.165) was created. RNA132 inhibits enzymatic activity of RVV-X. The effector molecule, VEGF.sub.165, reverses the inhibitory activity of RNA132 on RVV-X and restores its enzymatic activity, thus triggering the cascade and enabling the phase transition. Similar results were obtained for another allosteric aptamer modulated by a protein tyrosine phosphatase. The assay is instrumentation-free for both processing and readout.
Colorimetric aptasensor using unmodified gold nanoparticles for homogeneous multiplex detection.
Shucao Niu
Full Text Available Colorimetric aptasensors using unmodified gold nanoparticles (AuNPs have attracted much attention because of their low cost, simplicity, and practicality, and they have been developed for various targets in the past several years. However, previous research has focused on developing single-target assays. Here, we report the development of a homogeneous multiplex aptasensor by using more than one class of aptamers to stabilize AuNPs. Using sulfadimethoxine (SDM, kanamycin (KAN and adenosine (ADE as example targets, a KAN aptamer (750 nM, an SDM aptamer (250 nM and an ADE aptamer (500 nM were mixed at a 1∶1∶1 volume ratio and adsorbed directly onto the surface of unmodified AuNPs by electrostatic interaction. Upon the addition of any of the three targets, the conformation of the corresponding aptamer changed from a random coil structure to a rigid folded structure, which could not adsorb and stabilize AuNPs. The AuNPs aggregated in a specific reaction buffer (20 mM Tris-HCl containing 20 mM NaCl and 5 mM KCl, which led to a color change from red to purple/blue. These results demonstrate that the multiplex colorimetric aptasensor detected three targets simultaneously while maintaining the same sensitivity as a single-target aptasensor for each individual target. The multiplex aptasensor could be extended to other aptamers for various molecular detection events. Due to its simple design, easy operation, fast response, cost effectiveness and lack of need for sophisticated instrumentation, the proposed strategy provides a powerful tool to examine large numbers of samples to screen for a small number of potentially positive samples containing more than one analyte, which can be further validated using sophisticated instruments.
Homogeneity of passively ventilated waste tanks
Huckaby, J.L.; Jensen, L.; Cromar, R.D.; Hayes, J.C. [and others
1997-07-01
Gases and vapors in the high-level radioactive waste underground storage tanks at the Hanford Site are being characterized to help resolve waste storage safety issues and estimate air emissions. Characterization is accomplished by collecting and analyzing air samples from the headspaces of the tanks. Samples are generally collected from a single central location within the headspace, and it is assumed that they are representative of the entire headspace. The validity of this assumption appears to be very good for most tanks, because thermally induced convection currents within the headspaces mix constituents continuously. In the coolest waste tanks, however, thermally induced convection may be suppressed for several months of each year because of the seasonal soil temperature cycle. To determine whether composition does vary significantly with location in a cool tank, the headspaces of three waste tanks have been sampled at different horizontal and vertical locations during that part of the year when thermally induced convection is minimized. This report describes the bases for tank selection and the sampling and analytical methods used, then analyzes and discusses the results. Headspace composition data from two risers at three elevations in Tanks 241-B-103, TY-103, and U-112 have been analyzed by standard analysis of variance (ANOVA) methods, which indicate that these tank headspaces are essentially homogeneous. No stratification of denser vapors (e.g., carbon tetrachloride, dodecane) or lighter gases (e.g., ammonia, hydrogen) was detected in any of the three tanks. A qualitative examination of all tentatively identified organic vapors in SUMMA{trademark} and TST samples supported this conclusion.
Homogeneous and heterogenized iridium water oxidation catalysts
Macchioni, Alceo
2014-10-01
The development of an efficient catalyst for the oxidative splitting of water into molecular oxygen, protons and electrons is of key importance for producing solar fuels through artificial photosynthesis. We are facing the problem by means of a rational approach aimed at understanding how catalytic performance may be optimized by the knowledge of the reaction mechanism of water oxidation and the fate of the catalytic site under the inevitably harsh oxidative conditions. For the purposes of our study we selected iridium water oxidation catalysts, exhibiting remarkable performance (TOF > 5 s-1 and TON > 20000). In particular, we recently focused our attention on [Cp*Ir(N,O)X] (N,O = 2-pyridincarboxylate; X = Cl or NO3) and [IrCl(Hedta)]Na water oxidation catalysts. The former exhibited a remarkable TOF whereas the latter showed a very high TON. Furthermore, [IrCl(Hedta)]Na was heterogenized onto TiO2 taking advantage of the presence of a dandling -COOH functionality. The heterogenized catalyst maintained approximately the same catalytic activity of the homogeneous analogous with the advantage that could be reused many times. Mechanistic studies were performed in order to shed some light on the rate-determining step and the transformation of catalysts when exposed to "oxidative stress". It was found that the last oxidative step, preceding oxygen liberation, is the rate-determining step when a small excess of sacrificial oxidant is used. In addition, several intermediates of the oxidative transformation of the catalyst were intercepted and characterized by NMR, X-Ray diffractometry and ESI-MS.
Homogenization in strength and durability analysis of reinforced tooth filling
Mikhailov, SE; Orlik, J
2002-01-01
An asymptotic homogenization procedure is employed to obtain effective elastic properties of the composite tooth filling, a homogenized macro– stress field and a first approximation to the micro-stress field, from properties of the components and applied macro–loads. Using the approximate micro–stress field, a non–local initial strength and fatigue durability macro–conditions for the composite filling material is expressed in terms of the homogenized macro–stresses. An illustrative example wi...
Quantitative determination of homogeneous strain value in superplastic tension
无
2002-01-01
After load instability, a passage of homogeneous strain ε can be still continued in superplastic tensile deformation. But untill now, no one has given the precise value of ε corresponding to actual materials, neither in experimental measurements nor in theoretical calculations. Using the elaborate experimental measuring methods of m value and its function expressions, the note first gives the method to determine homogeneous ε, and the homogeneous ε value of typical superplastic alloy Zn-5% Al under 18℃ and 340℃ respectively.
Homogenization of alloys in the system Al-Mg-Sc
Drits, M. E.; Toropova, L. S.; Bykov, Yu. G.
1983-07-01
A reduction in homogenizing anneal temperature and a reduction in soaking time leads to an increase in yield point for alloys of the system Al-Mg-Sc. The optimum homogenizing schedule for alloys of this system is heating to 400°C and soaking for up to 10 h. This homogenizing schedule provides an alloy structure of fine coherent ScAl3-phase precipitates and dissolution of excess β-phase precipitates.
A fluorescence anisotropy assay for the muscarinic M1 G-protein-coupled receptor.
Huwiler, Kristin G; De Rosier, Therese; Hanson, Bonnie; Vogel, Kurt W
2010-06-01
In the search for new chemical entities that interact with G-proteincoupled receptors (GPCRs), assays that quantify efficacy and affinity are employed. Traditional methods for measuring affinity involve radiolabeled ligands. To address the need for homogeneous biochemical fluorescent assays to characterize orthosteric ligand affinity and dissociation rates, we have developed a fluorescence anisotropy (FA) assay for the muscarinic M1 receptor that can be conducted in a 384-well plate. We used membranes from a muscarinic M1 cell line optimized for high-throughput functional assays and the previously characterized fluorescent antagonist BODIPY FL pirenzepine. The affinities of reference compounds were determined in the competitive FA assay and compared with those obtained with a competitive filter-based radioligand-binding assay using [(3)H] N-methylscopolamine. The IC(50) values produced from the FA assay were well-correlated with the radioligand-binding K(i) values (R(2) = 0.98). The dissociation of the BODIPY FL pirenzepine was readily monitored in real time using the FA assay and was sensitive to the presence of the allosteric modulator gallamine. This M1 FA assay offers advantages over traditional radioligandbinding assays as it eliminates radioactivity while allowing investigation of orthosteric or allosteric muscarinic M1 ligands in a homogeneous format.
Homogenization Theory for a Replenishing Passive Scalar Field
Peter R.KRAMER; Shane R.KEATING
2009-01-01
Homogenization theory provides a rigorous framework for calculating the effective diffusivity of a decaying passive scalar field in a turbulent or complex flow.The authors extend this framework to the case where the passive scalar fluctuations ore continuously replenished by a source(and/or sink).The basic structure.of the homogenized equations carries over,but in some eases the homogenized source can involve a non-trivial coupling of the velocity field and the source.The authors derive expressions for the homogenized source term for various multiscale source structures and interpret them physically.
A non-asymptotic homogenization theory for periodic electromagnetic structures.
Tsukerman, Igor; Markel, Vadim A
2014-08-08
Homogenization of electromagnetic periodic composites is treated as a two-scale problem and solved by approximating the fields on both scales with eigenmodes that satisfy Maxwell's equations and boundary conditions as accurately as possible. Built into this homogenization methodology is an error indicator whose value characterizes the accuracy of homogenization. The proposed theory allows one to define not only bulk, but also position-dependent material parameters (e.g. in proximity to a physical boundary) and to quantify the trade-off between the accuracy of homogenization and its range of applicability to various illumination conditions.
Deterministic homogenization of parabolic monotone operators with time dependent coefficients
Gabriel Nguetseng
2004-06-01
Full Text Available We study, beyond the classical periodic setting, the homogenization of linear and nonlinear parabolic differential equations associated with monotone operators. The usual periodicity hypothesis is here substituted by an abstract deterministic assumption characterized by a great relaxation of the time behaviour. Our main tool is the recent theory of homogenization structures by the first author, and our homogenization approach falls under the two-scale convergence method. Various concrete examples are worked out with a view to pointing out the wide scope of our approach and bringing the role of homogenization structures to light.
Cook, Geoffrey M W; Jareonsettasin, Prem; Keynes, Roger J
2014-01-01
The growth cone collapse assay has proved invaluable in detecting and purifying axonal repellents. Glycoproteins/proteins present in detergent extracts of biological tissues are incorporated into liposomes, added to growth cones in culture and changes in morphology are then assessed. Alternatively purified or recombinant molecules in aqueous solution may be added directly to the cultures. In both cases after a defined period of time (up to 1 h), the cultures are fixed and then assessed by inverted phase contrast microscopy for the percentage of growth cones showing a collapsed profile with loss of flattened morphology, filopodia, and lamellipodia.
2007-01-01
The present invention relates to a device for use in performing assays on standard laboratory solid supports whereon chemical entities are attached. The invention furthermore relates to the use of such a device and a kit comprising such a device. The device according to the present invention is a......, when operatively connected, one or more chambers (21) comprising the chemical entities (41), the inlet(s) (5) and outlet(s) (6) and chambers (21) being in fluid connection. The device further comprise means for providing differing chemical conditions in each chamber (21)....
Rumleskie, Janet [Laurentian University, Greater Sudbury, Ontario (Canada)
2015-12-31
The SNO+ experiment will study neutrinos while located 6,800 feet below the surface of the earth at SNOLAB. Though shielded from surface backgrounds, emanation of radon radioisotopes from the surrounding rock leads to back-grounds. The characteristic decay of radon and its daughters allows for an alpha detection technique to count the amount of Rn-222 atoms collected. Traps can collect Rn-222 from various positions and materials, including an assay skid that will collect Rn-222 from the organic liquid scintillator used to detect interactions within SNO+.
Markov Chain Computation for Homogeneous and Non-homogeneous Data: MARCH 1.1 Users Guide
Andre Berchtold
2001-03-01
Full Text Available MARCH is a free software for the computation of different types of Markovian models including homogeneous Markov Chains, Hidden Markov Models (HMMs and Double Chain Markov Models (DCMMs. The main characteristic of this software is the implementation of a powerful optimization method for HMMs and DCMMs combining a genetic algorithm with the standard Baum-Welch procedure. MARCH is distributed as a set of Matlab functions running under Matlab 5 or higher on any computing platform. A PC Windows version running independently from Matlab is also available.
RAS - Screens & Assays - Drug Discovery
The RAS Drug Discovery group aims to develop assays that will reveal aspects of RAS biology upon which cancer cells depend. Successful assay formats are made available for high-throughput screening programs to yield potentially effective drug compounds.
Homogeneous Broadening of Optical Transitions in Organic Mixed Crystals
Vries, Harmen de; Wiersma, Douwe A.
1976-01-01
We have used the phenomenon of laser-induced molecular photodissociation to determine the homogeneous linewidth at 2 K of the origin (zero-phonon line) and a vibronic transition in the mixed-crystal absorption spectrum of dimethyl s-tetrazine in durene. From the measured 55-MHz (upper limit) homogen
Large-scale Homogenization of Bulk Materials in Mammoth Silos
Schott, D.L.
2004-01-01
This doctoral thesis concerns the large-scale homogenization of bulk materials in mammoth silos. The objective of this research was to determine the best stacking and reclaiming method for homogenization in mammoth silos. For this purpose a simulation program was developed to estimate the homogeniza
Homogeneous Buchberger algorithms and Sullivant's computational commutative algebra challenge
Lauritzen, Niels
2005-01-01
We give a variant of the homogeneous Buchberger algorithm for positively graded lattice ideals. Using this algorithm we solve the Sullivant computational commutative algebra challenge.......We give a variant of the homogeneous Buchberger algorithm for positively graded lattice ideals. Using this algorithm we solve the Sullivant computational commutative algebra challenge....
Non-linear waves in heterogeneous elastic rods via homogenization
Quezada de Luna, Manuel
2012-03-01
We consider the propagation of a planar loop on a heterogeneous elastic rod with a periodic microstructure consisting of two alternating homogeneous regions with different material properties. The analysis is carried out using a second-order homogenization theory based on a multiple scale asymptotic expansion. © 2011 Elsevier Ltd. All rights reserved.
Calderon type reproducing formula on spaces of homogeneous type
邓东皋; 韩永生
1995-01-01
By using the Calderon-Zygmund operator theory, a continuous version of the Calderon type reproducing formula associated to a para-accretive function on spaces of homogeneous type is proved. A new characterization of the Besov and Triebel-Lizorkin spaces on spaces of homogeneous type is also obtained.
Feeding premature infants banked human milk homogenized by ultrasonic treatment.
Rayol, M R; Martinez, F E; Jorge, S M; Gonçalves, A L; Desai, I D
1993-12-01
Premature neonates fed ultrasonically homogenized human milk had better weight gain and triceps skin-fold thickness than did a control group given untreated human milk (p homogenization of human milk appears to minimize loss of fat and thus allows better growth of premature infants.
Characterization of isolated homogeneous hypersurface singularities in C4
LIN; Kepao; TU; Zhenhan; YAU; Stephen; S; T
2006-01-01
Let V be a hypersurface with an isolated singularity at the origin in Cn+1. It is a natural question to ask when V is defined by weighted homogeneous polynomial or homogeneous polynomial up to biholomorphic change of coordinates. In 1971, a beautiful theorem of Saito gives a necessary and sufficient condition for V to be defined by a weighted homogeneous polynomial.For a two-dimensional isolated hypersurface singularity V, Xu and Yau found a coordinate free characterization for V to be defined by a homogeneous polynomial. Recently Lin and Yau gave necessary and sufficient conditions for a 3-dimensional isolated hypersurface singularity with geometric genus bi.er than zero to be defined by a homogeneous polynomial. The purpose of this paper is to prove that Lin-Yau's theorem remains true for singularities with geometric genus equal to zero.
Scale-dependent homogeneity measures for causal dynamical triangulations
Cooperman, Joshua H
2014-01-01
I propose two scale-dependent measures of the homogeneity of the quantum geometry determined by an ensemble of causal triangulations. The first measure is volumetric, probing the growth of volume with graph geodesic distance. The second measure is spectral, probing the return probability of a random walk with diffusion time. Both of these measures, particularly the first, are closely related to those used to assess the homogeneity of our own universe on the basis of galaxy redshift surveys. I employ these measures to quantify the quantum spacetime homogeneity as well as the temporal evolution of quantum spatial homogeneity of ensembles of causal triangulations in the well-known physical phase. According to these measures, the quantum spacetime geometry exhibits some degree of inhomogeneity on sufficiently small scales and a high degree of homogeneity on sufficiently large scales. This inhomogeneity appears unrelated to the phenomenon of dynamical dimensional reduction. I also uncover evidence for power-law sc...
Fibrations and globalizations of compact homogeneous CR-manifolds
Gilligan, B.; Huckleberry, Alan T.
2009-06-01
Fibration methods which were previously used for complex homogeneous spaces and CR-homogeneous spaces of special types [1]-[4] are developed in a general framework. These include the \\mathfrak{g}-anticanonical fibration in the CR-setting, which reduces certain considerations to the compact projective algebraic case, where a Borel-Remmert type splitting theorem is proved. This leads to a reduction to spaces homogeneous under actions of compact Lie groups. General globalization theorems are proved which enable one to regard a homogeneous CR-manifold as an orbit of a real Lie group in a complex homogeneous space of a complex Lie group. In the special case of CR-codimension at most two, precise classification results are proved and are applied to show that in most cases there exists such a globalization.
Fibrations and globalizations of compact homogeneous CR-manifolds
Gilligan, B [University of Regina, Regina (Canada); Huckleberry, Alan T [Ruhr-Universitaet Bochum, Mathematischer Institut, Bochum (Germany)
2009-06-30
Fibration methods which were previously used for complex homogeneous spaces and CR-homogeneous spaces of special types [1]-[4] are developed in a general framework. These include the g-anticanonical fibration in the CR-setting, which reduces certain considerations to the compact projective algebraic case, where a Borel-Remmert type splitting theorem is proved. This leads to a reduction to spaces homogeneous under actions of compact Lie groups. General globalization theorems are proved which enable one to regard a homogeneous CR-manifold as an orbit of a real Lie group in a complex homogeneous space of a complex Lie group. In the special case of CR-codimension at most two, precise classification results are proved and are applied to show that in most cases there exists such a globalization.
Bacterial assays for recombinagens.
Hoffmann, G R
1992-12-01
Two principal strategies have been used for studying recombinagenic effects of chemicals and radiation in bacteria: (1) measurement of homologous recombination involving defined alleles in a partially diploid strain, and (2) measurement of the formation and loss of genetic duplications in the bacterial chromosome. In the former category, most methods involve one allele in the bacterial chromosome and another in a plasmid, but it is also possible to detect recombination between two chromosomal alleles or between two extrachromosomal alleles. This review summarizes methods that use each of these approaches for detecting recombination and tabulates data on agents that have been found to be recombinagenic in bacteria. The assays are discussed with respect to their effectiveness in testing for recombinagens and their potential for elucidating mechanisms underlying recombinagenic effects.
Turbulent Diffusion in Non-Homogeneous Environments
Diez, M.; Redondo, J. M.; Mahjoub, O. B.; Sekula, E.
2012-04-01
Many experimental studies have been devoted to the understanding of non-homogeneous turbulent dynamics. Activity in this area intensified when the basic Kolmogorov self-similar theory was extended to two-dimensional or quasi 2D turbulent flows such as those appearing in the environment, that seem to control mixing [1,2]. The statistical description and the dynamics of these geophysical flows depend strongly on the distribution of long lived organized (coherent) structures. These flows show a complex topology, but may be subdivided in terms of strongly elliptical domains (high vorticity regions), strong hyperbolic domains (deformation cells with high energy condensations) and the background turbulent field of moderate elliptic and hyperbolic characteristics. It is of fundamental importance to investigate the different influence of these topological diverse regions. Relevant geometrical information of different areas is also given by the maximum fractal dimension, which is related to the energy spectrum of the flow. Using all the available information it is possible to investigate the spatial variability of the horizontal eddy diffusivity K(x,y). This information would be very important when trying to model numerically the behaviour in time of the oil spills [3,4] There is a strong dependence of horizontal eddy diffusivities with the Wave Reynolds number as well as with the wind stress measured as the friction velocity from wind profiles measured at the coastline. Natural sea surface oily slicks of diverse origin (plankton, algae or natural emissions and seeps of oil) form complicated structures in the sea surface due to the effects of both multiscale turbulence and Langmuir circulation. It is then possible to use the topological and scaling analysis to discriminate the different physical sea surface processes. We can relate higher orden moments of the Lagrangian velocity to effective diffusivity in spite of the need to calibrate the different regions determining the
First 25-hydroxyvitamin D assay for general chemistry analyzers.
Saida, Fakhri B; Chen, Xiaoru; Tran, Kiet; Dou, Chao; Yuan, Chong
2015-03-01
25-Hydroxyvitamin D [25(OH)D], the predominant circulating form of vitamin D, is an accurate indicator of the general vitamin D status of an individual. Because vitamin D deficiencies have been linked to several pathologies (including osteoporosis and rickets), accurate monitoring of 25(OH)D levels is becoming increasingly important in clinical settings. Current 25(OH)D assays are either chromatographic or immunoassay-based assays. These assays include HPLC, liquid chromatography-tandem mass spectrometry (LC-MS/MS), enzyme-immunosorbent, immunochemiluminescence, immunofluorescence and radioimmunoassay. All these assays use heterogeneous formats that require phase separation and special instrumentations. In this article, we present an overview of these assays and introduce the first homogeneous assay of 25(OH)D for use on general chemistry analyzers. A special emphasis is put on the unique challenges posed by the 25(OH)D analyte. These challenges include a low detection limit, the dissociation of the analyte from its serum transporter and the inactivation of various binding proteins without phase separation steps.
Escher, A.; Boulouchos, K.
2007-07-01
This annual report for the Swiss Federal Office of Energy (SFOE), reports on work done in 2007 at the Swiss Federal Institute of Technology ETH in Zurich, Switzerland, on fuels for homogenous, self-igniting combustion processes. A single-stroke engine was used to test the combustion of n-heptane, n-butane and their combinations. The flexibility of the test-bed is discussed and the combustion characteristics observed are described. Also, the results obtained are presented and discussed. Multi-zone simulation and factors influencing the combustion are examined. Ignition and combustion of synthetic, diesel-like fuels are discussed. Co-operation with the project started in 2007 by the Society for Research on Combustion Engines which involves other universities and several industrial companies is noted.
Peripheral nerve magnetic stimulation: influence of tissue non-homogeneity
Papazov Sava P
2003-12-01
Full Text Available Abstract Background Peripheral nerves are situated in a highly non-homogeneous environment, including muscles, bones, blood vessels, etc. Time-varying magnetic field stimulation of the median and ulnar nerves in the carpal region is studied, with special consideration of the influence of non-homogeneities. Methods A detailed three-dimensional finite element model (FEM of the anatomy of the wrist region was built to assess the induced currents distribution by external magnetic stimulation. The electromagnetic field distribution in the non-homogeneous domain was defined as an internal Dirichlet problem using the finite element method. The boundary conditions were obtained by analysis of the vector potential field excited by external current-driven coils. Results The results include evaluation and graphical representation of the induced current field distribution at various stimulation coil positions. Comparative study for the real non-homogeneous structure with anisotropic conductivities of the tissues and a mock homogeneous media is also presented. The possibility of achieving selective stimulation of either of the two nerves is assessed. Conclusion The model developed could be useful in theoretical prediction of the current distribution in the nerves during diagnostic stimulation and therapeutic procedures involving electromagnetic excitation. The errors in applying homogeneous domain modeling rather than real non-homogeneous biological structures are demonstrated. The practical implications of the applied approach are valid for any arbitrary weakly conductive medium.
Herbicide resistance screening assay.
Peterson, Joan M
2009-01-01
Herbicide resistance screening is a method that can be used not only to determine presence of the enzyme, phosphinothricin acetyltransferase, encoded by either the Bar or the Pat gene in transgenic maize, but also to assess the inheritance ratio of those genes in a segregating population. Herbicide screening can also be used to study linkage of a transgene of interest that was cotransformed with the herbicide resistance marker gene. By combining the herbicide screen assay with a PCR-based screen of leaf tissue DNA for the presence of both the Bar or the Pat gene marker and a cotransformed transgene of interest from the same seedling tissue and maintaining that seedling identity, the researcher can identify linkage or the possible breakdown in linkage of the marker gene and the transgene of interest. Further, the occurrence of "DNA silencing" can be evaluated if an individual seedling that was susceptible to the applied herbicide nonetheless gave PCR data that indicated presence of the gene responsible for herbicide resistance. Similarly, "DNA silencing" of the gene of interest may be investigated if the seeds can be screened and scored for that phenotypic trait in a nondestructive manner prior to planting.
Stability properties of autonomous homogeneous polynomial differential systems
Samardzija, Nikola
A geometrical approach is used to derive a generalized characteristic value problem for dynamic systems described by homogeneous polynomials. It is shown that a nonlinear homogeneous polynomial system possesses eigenvectors and eigenvalues, quantities normally associated with a linear system. These quantities are then employed in studying stability properties. The necessary and sufficient conditions for all forms of stabilities characteristic of a two-dimensional system are provided. This result, together with the classical theorem of Frommer, completes a stability analysis for a two-dimensional homogeneous polynomial system.
Killing vector fields and a homogeneous isotropic universe
Katanaev, M O
2016-01-01
Some basic theorems on Killing vector fields are reviewed. In particular, the topic of a constant-curvature space is examined. A detailed proof is given for a theorem describing the most general form of the metric of a homogeneous isotropic space-time. Although this theorem can be considered to be commonly known, its complete proof is difficult to find in the literature. An example metric is presented such that all its spatial cross sections correspond to constant-curvature spaces, but it is not homogeneous and isotropic as a whole. An equivalent definition of a homogeneous and isotropic space-time in terms of embedded manifolds is also given.
Analyses of beyond design basis accident homogeneous boron dilution scenarios
Kereszturi, Andras; Hegyi, Gyoergy; Maraczy, Csaba; Trosztel, Istvan; Tota, Adam [Hungarian Academy of Sciences, Centre for Energy Research, Budapest (Hungary); Karsa, Zoltan [NUBIKI Nuclear Safety Research Institute, Ltd., Budapest (Hungary)
2015-09-15
Homogeneous boron dilution scenarios in a VVER-440 reactor were analyzed using the coupled KIKO3D-ATHLET code. The scenarios are named ''homogeneous'' because of the very slow dilution caused by a rupture in the heat exchanger of the makeup system. Without the presented analyses, a significant contribution of the homogeneous boron dilution to the Core Damage Frequency (CDF) had to be assumed in the Probabilistic Safety Analyses (PSA). According to the combined results of the presented deterministic and probabilistic analyses, the final conclusion is that boron dilution transients don't give significant contribution to the CDF for the investigated VVER-440 NPP.
Synthesis of silica nanosphere from homogeneous and heterogeneous systems
N Venkatathri
2007-12-01
Silica nanosphere was synthesized using homogeneous and heterogeneous systems, respectively. In homogeneous system, silica spheres were synthesized without cetyltrimethylammonium bromide (CTABr), which gave bimodal particle size and lower yield (77%). To improve the yield, CTABr was added and found that the yield was very high (100%). The particle was in nm range, but the particle sizes are bimodal. To avoid it, reaction in heterogeneous system using CTABr was carried out. Nanosized silica sphere with uniform size (yield, 94%) was observed. Homogeneous system contains a mixture of ethanol, water, aqueous ammonia and tetraethylorthosilicate (TEOS). In the case of heterogeneous system, only ethanol was absent.
Homogenization and structural topology optimization theory, practice and software
Hassani, Behrooz
1999-01-01
Structural topology optimization is a fast growing field that is finding numerous applications in automotive, aerospace and mechanical design processes. Homogenization is a mathematical theory with applications in several engineering problems that are governed by partial differential equations with rapidly oscillating coefficients Homogenization and Structural Topology Optimization brings the two concepts together and successfully bridges the previously overlooked gap between the mathematical theory and the practical implementation of the homogenization method. The book is presented in a unique self-teaching style that includes numerous illustrative examples, figures and detailed explanations of concepts. The text is divided into three parts which maintains the book's reader-friendly appeal.
Wu, R; Felton, J
2007-10-12
The Ames/Salmonella assay, developed by Professor Bruce Ames at the University of California, Berkeley, is a rapid and sensitive assay for detecting mutagenicity of various chemical compounds (Maron and Ames, 1983). It is a widely accepted short-term assay for detecting chemicals that induce mutations in the histidine (his) gene of Salmonella typhimurium. This is a reverse mutation assay that detects the mutational reversion of his-dependent Salmonella to the his-independent counterpart. Thereby, mutagenic compounds will increase the frequency of occurrence of his-independent bacterial colonies. The assay utilizes the specific genetically constructed strains of bacteria either with or without mammalian metabolic activation enzymes (S9), Aroclor induced rat liver homogenate to assess the mutagenicity of different compounds. In this study, we will use the Ames/Salmonella assay to investigate the mutagenicity of Hexanitrostilbene (HNS) from both Bofors and Pantex, and Dipicryethane (DPE).
Disagreement between Human Papillomavirus Assays
Rebolj, Matejka; Preisler, Sarah; Ejegod, Ditte Møller
2014-01-01
assays. Positive agreement between the assays was measured as the conditional probability that the results of all compared assays were positive given that at least one assay returned a positive result. Of all 5,064 samples, 1,679 (33.2%) tested positive on at least one of the assays. Among these, 41......We aimed to determine the disagreement in primary cervical screening between four human papillomavirus assays: Hybrid Capture 2, cobas, CLART, and APTIMA. Material from 5,064 SurePath samples of women participating in routine cervical screening in Copenhagen, Denmark, was tested with the four......% tested positive on all four. Agreement was lower in women aged ≥ 30 years (30%, vs. 49% at samples (29%, vs. 38% in follow-up samples), and in women with concurrent normal cytology (22%, vs. 68% with abnormal cytology). Among primary screening samples from women aged 30...
Dobslaff, Kristin; Zscharnack, Kristin; Kreisig, Thomas; Zuchner, Thole
2016-02-01
Immunoassays play an essential role in current research and diagnostics resulting in a variety of detection principles. Thereby, homogeneous assays are often used for a fast signal response as demanded for example in point-of-care diagnostics. These systems often rely on a competitive assay design where the sample analyte and the corresponding dye-labeled substance are competing for binding sites on an antibody present in limited amounts. Due to the similar affinities of the antibody towards the sample analyte and the competitor, both sensitivity and assay time are limited. As a consequence, a competitor with a slightly reduced affinity towards the antibody can potentially overcome these drawbacks. Here, we present the rational design of a low-affinity peptide (donor peptide) as a specific analyte competitor for a FRET-based homogeneous immunoassay for the analysis of the protein cystatin C. Thereby, the strategy of peptide-induced antibody generation was combined with the selective variation of the immunization sequence in order to achieve a lower affinity towards the antibody. We could show that shortened donor peptides improved the resulting quenching efficiency in the immunoassay. In addition, the substitution of small hydrophobic amino acids by those with a higher steric demand appeared to be the most promising strategy providing a fast assay response for cystatin C of only 90 s.
Siddiqui, A.K. (Atomic Energy Centre, Dacca (Bangladesh)); Ando, Y.; Karashimada, T.; Kameyama, K.
1979-09-01
Cod homogenates inoculated with spores of C. botulinum type E strain Erimo at 10/sup 2/ and 10/sup 4//g were treated with 1% and 2% sodium chloride, 0.25% each of mustard, garlic and turmeric and 0.3 Mrad ..gamma..-radiation either in single or combination treatments. The growth and toxin production of type E spores in the inoculated homogenates were followed at incubation temperatures of 30/sup 0/, 10/sup 0/ and 5/sup 0/C for 7, 28 and 56 days respectively. Growth and gas formation were noted in all the samples but type E toxin could not be detected. The reason for the absence of toxin in both the untreated and treated homogenates could not be ascertained. Inadequate detection method, unfavourable growth conditions in the homogenate and weak toxigenicity of the strain employed have been advanced as probable factors that contributed to the negative results on the toxin assay.
Bridging heterogeneous and homogeneous catalysis concepts, strategies, and applications
Li, Can
2014-01-01
This unique handbook fills the gap in the market for an up-to-date work that links both homogeneous catalysis applied to organic reactions and catalytic reactions on surfaces of heterogeneous catalysts.
Reconsideration on Homogeneous Quadratic Riemann Boundary Value Problem
Lu Jian-ke
2004-01-01
The homogeneous quadratic Riemann boundary value problem (1) with Hǒlder continuous coefficients for the normal case was considered by the author in 1997. But the solutions obtained there are incomplete. Here its general method of solution is obtained.
Extended Homogeneous Balance Method and Lax Pairs, Backlund Transformation
BAI ChengLin
2002-01-01
Using the extended homogeneous balance method, which is very concise and primary, Lax pairs and Backlund transformation for most nonlinear evolution equations, such as the compound KdV-Burgers equation and nonlinear diffusion equation are obtained.
On acoustic band gaps in homogenized piezoelectric phononic materials
Rohan E.
2010-07-01
Full Text Available We consider a composite medium made of weakly piezoelectric inclusions periodically distributed in the matrix which ismade of a different piezoelectricmaterial. Themediumis subject to a periodic excitation with an incidence wave frequency independent of scale ε of the microscopic heterogeneities. Two-scale method of homogenization is applied to obtain the limit homogenized model which describes acoustic wave propagation in the piezoelectric medium when ε → 0. In analogy with the purely elastic composite, the resulting model allows existence of the acoustic band gaps. These are identified for certain frequency ranges whenever the so-called homogenized mass becomes negative. The homogenized model can be used for band gap prediction and for dispersion analysis for low wave numbers. Modeling such composite materials seems to be perspective in the context of Smart Materials design.
Fractal Dimension as a measure of the scale of Homogeneity
Yadav, Jaswant K; Khandai, Nishikanta
2010-01-01
In the multi-fractal analysis of large scale matter distribution, the scale of transition to homogeneity is defined as the scale above which the fractal dimension of underlying point distribution is equal to the ambient dimension of the space in which points are distributed. With finite sized weakly clustered distribution of tracers obtained from galaxy redshift surveys it is difficult to achieve this equality. Recently we have defined the scale of homogeneity to be the scale above which the deviation of fractal dimension from the ambient dimension becomes smaller than the statistical dispersion. In this paper we use the relation between the fractal dimensions and the correlation function to compute the dispersion for any given model in the limit of weak clustering amplitude. We compare the deviation and dispersion for the LCDM model and discuss the implication of this comparison for the expected scale of homogeneity in the concordant model of cosmology. We estimate the upper limit to the scale of homogeneity...
Spatial variability of chloride in concrete within homogeneously exposed areas
Angst, U.M.; Polder, R.B.
2014-01-01
The concept of variability is increasingly considered in service life predictions. This paper reports experimental data on the spatial distribution of chloride in uncracked concrete subjected to homogeneous exposure. Chloride concentrations were measured with potentiometric sensors embedded in concr
Homogenization of attractors for a class of nonlinear parabolic equations
WANG Guo-lian; ZHANG Xing-you
2004-01-01
The relation between the global attractors Aε for a calss of quasilinear parabolic equations and the global attractor A0for the homogenized equation is discussed, and an explicit error estimate between Aε and A0 is given.
Homogenization of temperature field and temperature gradient field
无
2009-01-01
The homogenization of temperature field and temperature gradient field are very important for many devices, systems and equipments, such as satellites and electronic devices. This paper discusses the distribution optimization of the limited high conductivity material with the simulated annealing algorithm to homogenize the temperature field in a two-dimensional heat conduction problem. At the same time, the temperature gradient field is homogenized with the bionic optimization method. The results show that the two optimization targets are consistent to some extent, while the bionic optimization method could save much computing time. In addition, there are threshold values for the amount of high conductivity material and the ratio of the high conductivity to the low conductivity beyond which further increasing these values brings very little improvement on the homogenization of temperature field and temperature gradient field.
Orbits in Homogeneous Oblate Spheroidal Gravitational Space-Time
Chifu E. N.
2009-07-01
Full Text Available The generalized Lagrangian in general relativistic homogeneous oblate spheroidal gravitational fields is constructed and used to study orbits exterior to homogenous oblate spheroids. Expressions for the conservation of energy and angular momentum for this gravitational field are obtained. The planetary equation of motion and the equation of motion of a photon in the vicinity of an oblate spheroid are derived. These equations have additional terms not found in Schwarzschild's space time.
On homogeneity and its application in sliding mode control
Bernuau, Emmanuel; Efimov, Denis; Perruquetti, Wilfrid; Polyakov, Andrey
2014-01-01
International audience; The paper is reviewing the tools to handle high-order sliding mode design and robustness. The main ingredient is homogeneity which can be checked using an algebraic test and which helps us in obtaining one of the most desired properties in sliding mode control that is finite-time stability. This paper stresses some recently obtained results about homogeneity for differential inclusions and robustness with respect to perturbations in the context of input-to-state stabil...
Homogenization of aligned “fuzzy fiber” composites
Chatzigeorgiou, George
2011-09-01
The aim of this work is to study composites in which carbon fibers coated with radially aligned carbon nanotubes are embedded in a matrix. The effective properties of these composites are identified using the asymptotic expansion homogenization method in two steps. Homogenization is performed in different coordinate systems, the cylindrical and the Cartesian, and a numerical example are presented. © 2011 Elsevier Ltd. All rights reserved.
Matrix-dependent multigrid-homogenization for diffusion problems
Knapek, S. [Institut fuer Informatik tu Muenchen (Germany)
1996-12-31
We present a method to approximately determine the effective diffusion coefficient on the coarse scale level of problems with strongly varying or discontinuous diffusion coefficients. It is based on techniques used also in multigrid, like Dendy`s matrix-dependent prolongations and the construction of coarse grid operators by means of the Galerkin approximation. In numerical experiments, we compare our multigrid-homogenization method with homogenization, renormalization and averaging approaches.
The Approach to Steady State Using Homogeneous and Cartesian Coordinates
D. F. Gochberg
2013-01-01
Full Text Available Repeating an arbitrary sequence of RF pulses and magnetic field gradients will eventually lead to a steady-state condition in any magnetic resonance system. While numerical methods can quantify this trajectory, analytic analysis provides significantly more insight and a means for faster calculation. Recently, an analytic analysis using homogeneous coordinates was published. The current work further develops this line of thought and compares the relative merits of using a homogeneous or a Cartesian coordinate system.
Homogeneous free-form directional backlight for 3D display
Krebs, Peter; Liang, Haowen; Fan, Hang; Zhang, Aiqin; Zhou, Yangui; Chen, Jiayi; Li, Kunyang; Zhou, Jianying
2017-08-01
Realization of a near perfect homogeneous secondary emission source for 3D display is proposed and demonstrated. The light source takes advantage of an array of free-form emission surface with a specially tailored light guiding structure, a light diffuser and Fresnel lens. A seamless and homogeneous directional emission is experimentally obtained which is essential for a high quality naked-eye 3D display.
Catalysis at the Homogeneous-Heterogeneous Chemistry Interface
Howard; Alper
2007-01-01
1 Results Significant progress has been made in recent years in developing efficient, atom economical catalytic reactions of potential applicability to the pharmaceutical, petrochemical, and commodity chemical business sectors. In some cases, homogeneous catalytic processes offer advantages, but in others the use of heterogenized homogeneous catalysis provides a competitive advantage concerning recyclability and catalyst recovery. This presentation will consider new approaches to cyclization reactions a...
Homogenization of an ensemble of interacting resonant scatterers
Schilder, N. J.; Sauvan, C.; Sortais, Y. R. P.; Browaeys, A.; Greffet, J.-J.
2017-07-01
We study theoretically the concept of homogenization in optics using an ensemble of randomly distributed resonant stationary atoms with density ρ . The ensemble is dense enough for the usual condition for homogenization, viz. ρ λ3≫1 , to be reached. Introducing the coherent and incoherent scattered powers, we define two criteria to define the homogenization regime. We find that when the excitation field is tuned in a broad frequency range around the resonance, neither of the criteria for homogenization is fulfilled, meaning that the condition ρ λ3≫1 is not sufficient to characterize the homogenized regime around the atomic resonance. We interpret these results as a consequence of the light-induced dipole-dipole interactions between the atoms, which implies a description of scattering in terms of collective modes rather than as a sequence of individual scattering events. Finally, we show that, although homogenization can never be reached for a dense ensemble of randomly positioned laser-cooled atoms around resonance, it becomes possible if one introduces spatial correlations in the positions of the atoms or nonradiative losses, such as would be the case for organic molecules or quantum dots coupled to a phonon bath.
Trend and Homogeneity Analysis of Precipitation in Iran
Majid Javari
2016-09-01
Full Text Available The main objective of this study is to examine trend and homogeneity through the analysis of rainfall variability patterns in Iran. The study presents a review on the application of homogeneity and seasonal time series analysis methods for forecasting rainfall variations. Trend and homogeneity methods are applied in the time series analysis from collecting rainfall data to evaluating results in climate studies. For the homogeneity analysis of monthly, seasonal and annual rainfall, homogeneity tests were used in 140 stations in the 1975–2014 period. The homogeneity of the monthly and annual rainfall at each station was studied using the autocorrelation (ACF, and the von Neumann (VN tests at a significance level of 0.05. In addition, the nature of the monthly and seasonal rainfall series in Iran was studied using the Kruskal-Wallis (KW test, the Thumb test (TT, and the least squares regression (LSR test at a significance level of 0.05. The present results indicate that the seasonal patterns of rainfall exhibit considerable diversity across Iran. Rainfall seasonality is generally less spatially coherent than temporal patterns in Iran. The seasonal variations of rainfall decreased significantly throughout eastern and central Iran, but they increased in the west and north of Iran during the studied interval. The present study comparisons among variations of patterns with the seasonal rainfall series reveal that the variability of rainfall can be predicted by the non-trended and trended patterns.
Li, Zhen-Xing; Xue, Wei; Guan, Bing-Tao; Shi, Fu-Bo; Shi, Zhang-Jie; Jiang, Hong; Yan, Chun-Hua
2013-02-07
Translation of homogeneous catalysis into heterogeneous catalysis is a promising solution to green and sustainable development in chemical industry. For this purpose, noble metal nanoparticles represent a new frontier in catalytic transformations. Many challenges remain for researchers to transform noble metal nanoparticles of heterogeneous catalytic active sites into ionic species of homogeneous catalytic active sites. We report here a successful design on translating homogeneous gold catalysis into a heterogeneous system with a clear understanding of the catalytic pathway. This study initiates a novel concept to immobilize a homogeneous catalyst based on electron transfer between supporting base and supported nanoparticles. Meanwhile, on the basis of theoretical calculation, it has deepened the understanding of the interactions between noble metal nanoparticles and the catalyst support.
Evidence for homogeneity of thromboxane A2 receptor using structurally different antagonists.
Swayne, G T; Maguire, J; Dolan, J; Raval, P; Dane, G; Greener, M; Owen, D A
1988-08-01
Nine structurally dissimilar thromboxane antagonists (SQ 29548, ICI 185282, AH 23848, BM 13505 (Daltroban), BM 13177 (Sulotroban), SK&F 88046, L-636499, L-640035 and a Bayer compound SK&F 47821) were studied for activity as thromboxane A2 receptor antagonists. The assays used were inhibition of responses induced by the thromboxane mimetic, U46619, on human washed platelet aggregation, rabbit platelet aggregation, rabbit aortic strip contraction, anaesthetised guinea-pig bronchoconstriction, and a radio-labelled ligand (125I-PTA-OH) binding assay as a measure of affinity for the human platelet receptor. The results of the present study, with activities spanning at least four orders of magnitude along with statistically significant correlations (at least P less than 0.01), strongly suggests that between assays, antagonists and species a homogenous population of thromboxane A2 receptors exists. This finding is in contrast to those of a close series of 13-azapinane antagonists studied by other workers which have suggested receptor heterogeneity.
Practical assay issues with the PERT/PBRT assay: a highly sensitive reverse transcriptase assay.
Chang, A; Dusing, S
2006-01-01
Product safety testing for retroviruses can be achieved by a panel of screening assays, including electron microscopy, viral gene specific PCRs, virus propagation, and detection of reverse transciptase activity. The application of PCR-based reverse transcriptase assays (PERT) that are approximately a million-fold more sensitive than conventional nucleotide incorporation assays in the testing of biologicals is described. Use of PERT assays can be applied to three areas: (i) screening for adventitious retrovirus contamination; (ii) detecting and quantifying endogenous viral particle load and (iii) monitoring levels of infectious retrovirus generation in cell lines that contain endogenous retroviruses.
Land-use intensification causes multitrophic homogenization of grassland communities
Gossner, Martin M.; Lewinsohn, Thomas M.; Kahl, Tiemo; Grassein, Fabrice; Boch, Steffen; Prati, Daniel; Birkhofer, Klaus; Renner, Swen C.; Sikorski, Johannes; Wubet, Tesfaye; Arndt, Hartmut; Baumgartner, Vanessa; Blaser, Stefan; Blüthgen, Nico; Börschig, Carmen; Buscot, Francois; Diekötter, Tim; Jorge, Leonardo Ré; Jung, Kirsten; Keyel, Alexander C.; Klein, Alexandra-Maria; Klemmer, Sandra; Krauss, Jochen; Lange, Markus; Müller, Jörg; Overmann, Jörg; Pašalić, Esther; Penone, Caterina; Perović, David J.; Purschke, Oliver; Schall, Peter; Socher, Stephanie A.; Sonnemann, Ilja; Tschapka, Marco; Tscharntke, Teja; Türke, Manfred; Venter, Paul Christiaan; Weiner, Christiane N.; Werner, Michael; Wolters, Volkmar; Wurst, Susanne; Westphal, Catrin; Fischer, Markus; Weisser, Wolfgang W.; Allan, Eric
2016-12-01
Land-use intensification is a major driver of biodiversity loss. Alongside reductions in local species diversity, biotic homogenization at larger spatial scales is of great concern for conservation. Biotic homogenization means a decrease in β-diversity (the compositional dissimilarity between sites). Most studies have investigated losses in local (α)-diversity and neglected biodiversity loss at larger spatial scales. Studies addressing β-diversity have focused on single or a few organism groups (for example, ref. 4), and it is thus unknown whether land-use intensification homogenizes communities at different trophic levels, above- and belowground. Here we show that even moderate increases in local land-use intensity (LUI) cause biotic homogenization across microbial, plant and animal groups, both above- and belowground, and that this is largely independent of changes in α-diversity. We analysed a unique grassland biodiversity dataset, with abundances of more than 4,000 species belonging to 12 trophic groups. LUI, and, in particular, high mowing intensity, had consistent effects on β-diversity across groups, causing a homogenization of soil microbial, fungal pathogen, plant and arthropod communities. These effects were nonlinear and the strongest declines in β-diversity occurred in the transition from extensively managed to intermediate intensity grassland. LUI tended to reduce local α-diversity in aboveground groups, whereas the α-diversity increased in belowground groups. Correlations between the β-diversity of different groups, particularly between plants and their consumers, became weaker at high LUI. This suggests a loss of specialist species and is further evidence for biotic homogenization. The consistently negative effects of LUI on landscape-scale biodiversity underscore the high value of extensively managed grasslands for conserving multitrophic biodiversity and ecosystem service provision. Indeed, biotic homogenization rather than local diversity
Context homogeneity facilitates both distractor inhibition and target enhancement.
Feldmann-Wüstefeld, Tobias; Schubö, Anna
2013-05-06
Homogeneous contexts were shown to result in prioritized processing of embedded targets compared to heterogeneous contexts (Duncan & Humphreys, 1989). The present experiment used behavioral and ERP measures to examine whether context homogeneity affects both enhancing relevant information and inhibiting irrelevant in contexts of varying homogeneity. Targets and distractors were presented laterally or on the vertical midline which allowed disentangling target- and distractor-related activity in the lateralized ERP (Hickey, diLollo, & McDonald, 2009). In homogeneous contexts, targets elicited an NT component from 150 ms on and a PD component from 200 ms on, showing early attention deployment at target locations and active suppression of distractors. In heterogeneous contexts, an NT component was also found from 150 ms on and PD was found from 250 ms on, suggesting delayed suppression of the distractor. Before 250 ms, distractors in heterogeneous contexts elicited a contralateral negativity, indicating attentional capture of the distractor prior to active suppression. In sum the present results suggest that top-down control of attention is more pronounced in homogeneous than in heterogeneous contexts.
Transporter assays and assay ontologies: useful tools for drug discovery.
Zdrazil, Barbara; Chichester, Christine; Zander Balderud, Linda; Engkvist, Ola; Gaulton, Anna; Overington, John P
2014-06-01
Transport proteins represent an eminent class of drug targets and ADMET (absorption, distribution, metabolism, excretion, toxicity) associated genes. There exists a large number of distinct activity assays for transport proteins, depending on not only the measurement needed (e.g. transport activity, strength of ligand–protein interaction), but also due to heterogeneous assay setups used by different research groups. Efforts to systematically organize this (divergent) bioassay data have large potential impact in Public-Private partnership and conventional commercial drug discovery. In this short review, we highlight some of the frequently used high-throughput assays for transport proteins, and we discuss emerging assay ontologies and their application to this field. Focusing on human P-glycoprotein (Multidrug resistance protein 1; gene name: ABCB1, MDR1), we exemplify how annotation of bioassay data per target class could improve and add to existing ontologies, and we propose to include an additional layer of metadata supporting data fusion across different bioassays.
Lye, Peter G.; Bradbury, Ronald; Lamb, David W.
Silica optical fibres were used to measure colour (mg anthocyanin/g fresh berry weight) in samples of red wine grape homogenates via optical Fibre Evanescent Field Absorbance (FEFA). Colour measurements from 126 samples of grape homogenate were compared against the standard industry spectrophotometric reference method that involves chemical extraction and subsequent optical absorption measurements of clarified samples at 520 nm. FEFA absorbance on homogenates at 520 nm (FEFA520h) was correlated with the industry reference method measurements of colour (R2 = 0.46, n = 126). Using a simple regression equation colour could be predicted with a standard error of cross-validation (SECV) of 0.21 mg/g, with a range of 0.6 to 2.2 mg anthocyanin/g and a standard deviation of 0.33 mg/g. With a Ratio of Performance Deviation (RPD) of 1.6, the technique when utilizing only a single detection wavelength, is not robust enough to apply in a diagnostic sense, however the results do demonstrate the potential of the FEFA method as a fast and low-cost assay of colour in homogenized samples.
Fluorescence Polarization Assays in Small Molecule Screening
Lea, Wendy A.; Simeonov, Anton
2011-01-01
Importance of the field Fluorescence polarization (FP) is a homogeneous method that allows rapid and quantitative analysis of diverse molecular interactions and enzyme activities. This technique has been widely utilized in clinical and biomedical settings, including the diagnosis of certain diseases and monitoring therapeutic drug levels in body fluids. Recent developments in the field has been symbolized by the facile adoption of FP in high-throughput screening (HTS) and small molecule drug discovery of an increasing range of target classes. Areas covered in this review The article provides a brief overview on the theoretical foundation of FP, followed by updates on recent advancements in its application for various drug target classes, including G-protein coupled receptors (GPCRs), enzymes and protein-protein interactions (PPIs). The strengths and weaknesses of this method, practical considerations in assay design, novel applications, and future directions are also discussed. What the reader will gain The reader will be informed of the most recent advancements and future directions of FP application to small molecule screening. Take home message In addition to its continued utilization in high-throughput screening, FP has expanded into new disease and target areas and has been marked by increased use of labeled small molecule ligands for receptor binding studies. PMID:22328899
Dynamic spreading behavior of homogeneous and heterogeneous networks
XIA Chengyi; LIU Zhongxin; CHEN Zengqiang; YUAN Zhuzhi
2007-01-01
The detailed investigation of the dynamic epidemic spreading on homogeneous and heterogeneous networks was carried out. After the analysis of the basic epidemic models, the susceptible-infected-susceptible (SIS) model on homogenous and heterogeneous networks is established, and the dynamical evolution of the density of the infected individuals in these two different kinds of networks is analyzed theoretically. It indicates that heterogeneous networks are easier to propagate for the epidemics and the leading spreading behavior is dictated by the exponential increasing in the initial outbreaks. Large-scale simulations display that the infection is much faster on heterogeneous networks than that on homogeneous ones. It means that the network topology can have a significant effect on the epidemics taking place on complex networks. Some containment strategies of epidemic outbreaks are presented according to the theoretical analyses and numerical simulations.
A tree-based model for homogeneous groupings of multinomials.
Yang, Tae Young
2005-11-30
The motivation of this paper is to provide a tree-based method for grouping multinomial data according to their classification probability vectors. We produce an initial tree by binary recursive partitioning whereby multinomials are successively split into two subsets and the splits are determined by maximizing the likelihood function. If the number of multinomials k is too large, we propose to order the multinomials, and then build the initial tree based on a dramatically smaller number k-1 of possible splits. The tree is then pruned from the bottom up. The pruning process involves a sequence of hypothesis tests of a single homogeneous group against the alternative that there are two distinct, internally homogeneous groups. As pruning criteria, the Bayesian information criterion and the Wilcoxon rank-sum test are proposed. The tree-based model is illustrated on genetic sequence data. Homogeneous groupings of genetic sequences present new opportunities to understand and align these sequences.
Homogeneous sample preparation of raw shrimp using dry ice.
Bunch, E A; Altwein, D M; Johnson, L E; Farley, J R; Hammersmith, A A
1995-01-01
Sample homogeneity is critical to accurate and reproducible analysis of trace residues in foods. A method of uniform sample preparation using dry ice is described for shrimp. Other sample preparation techniques for raw shrimp produce nonhomogeneous samples. Sample homogeneity was determined through analysis of chloramphenicol added to intact tiger or white shrimp prior to sample preparation. Simulated chloramphenicol residue levels were 50, 15, 10, and 5 ppb. No significant differences were noted when analyses of shrimp inoculated with chlor-amphenicol prior to sample preparation with dry ice were compared with analyses of shrimp spiked after grinding with dry ice. Grinding shrimp with dry ice produced samples with homogeneous chloramphenicol residues. This technique should be applicable to other tissues and vegetable products.
A Computationally Based Approach to Homogenizing Advanced Alloys
Jablonski, P D; Cowen, C J
2011-02-27
We have developed a computationally based approach to optimizing the homogenization heat treatment of complex alloys. The Scheil module within the Thermo-Calc software is used to predict the as-cast segregation present within alloys, and DICTRA (Diffusion Controlled TRAnsformations) is used to model the homogenization kinetics as a function of time, temperature and microstructural scale. We will discuss this approach as it is applied to both Ni based superalloys as well as the more complex (computationally) case of alloys that solidify with more than one matrix phase as a result of segregation. Such is the case typically observed in martensitic steels. With these alloys it is doubly important to homogenize them correctly, especially at the laboratory scale, since they are austenitic at high temperature and thus constituent elements will diffuse slowly. The computationally designed heat treatment and the subsequent verification real castings are presented.
Permutation-based Homogeneous Block Content Authentication for Watermarking
S.Maruthuperumal
2013-02-01
Full Text Available In modern days, digital watermarking has become an admired technique for hitting data in digital images to help guard against copyright infringement. The proposed Permutation-based Homogeneous Block Content authentication (PHBC methods develop a secure and excellence strong watermarking algorithm that combines the reward of permutation-based Homogeneous block (PHB with that of significant and insignificant bit values with X0R encryption function using Max coefficient of least coordinate value for embedding the watermark. In the projected system uses the relationship between the permutation blocks to embed many data into Homogeneous blocks without causing solemn distortion to the watermarked image. The experimental results show that the projected system is very efficient in achieving perceptual invisibility with an increase in the Peak Signal to Noise Ratio (PSNR. Moreover, the projected system is robust to a variety of signal processing operations, such as image Cropping, Rotation, Resizing, Adding noise, Filtering , Blurring and Motion blurring.
Classical and quantum dissipation in non homogeneous environments
Illuminati, F; Sodano, P
1994-01-01
We generalize the oscillator model of a particle interacting with a thermal reservoir by introducing arbitrary nonlinear couplings in the particle coordinates.The equilibrium positions of the heat bath oscillators are promoted to space-time functions, which are shown to represent a modulation of the internal noise by the external forces. The model thus provides a description of classical and quantum dissipation in non homogeneous environments. In the classical case we derive a generalized Langevin equation with nonlinear multiplicative noise and a position-dependent fluctuation- dissipation theorem associated to non homogeneous dissipative forces. When time-modulation of the noise is present, a new force term is predicted besides the dissipative and random ones. The model is quantized to obtain the non homogenous influence functional and master equation for the reduced density matrix of the Brownian particle. The quantum evolution equations reproduce the correct Langevin dynamics in the semiclassical limit. T...
Scaling of Lyapunov Exponents in Homogeneous, Isotropic DNS
Fitzsimmons, Nicholas; Malaya, Nicholas; Moser, Robert
2013-11-01
Lyapunov exponents measure the rate of separation of initially infinitesimally close trajectories in a chaotic system. Using the exponents, we are able to probe the chaotic nature of homogeneous isotropic turbulence and study the instabilities of the chaotic field. The exponents are measured by calculating the instantaneous growth rate of a linear disturbance, evolved with the linearized Navier-Stokes equation, at each time step. In this talk, we examine these exponents in the context of homogeneous isotropic turbulence with two goals: 1) to investigate the scaling of the exponents with respect to the parameters of forced homogeneous isotropic turbulence, and 2) to characterize the instabilities that lead to chaos in turbulence. Specifically, we explore the scaling of the Lyapunov exponents with respect to the Reynolds number and with respect to the ratio of the integral length scale and the computational domain size.
Defining least community as a homogeneous group in complex networks
Jiang, Bin; Ma, Ding
2015-06-01
This paper introduces a new concept of least community that is as homogeneous as a random graph, and develops a new community detection algorithm from the perspective of homogeneity or heterogeneity. Based on this concept, we adopt head/tail breaks-a newly developed classification scheme for data with a heavy-tailed distribution-and rely on edge betweenness given its heavy-tailed distribution to iteratively partition a network into many heterogeneous and homogeneous communities. Surprisingly, the derived communities for any self-organized and/or self-evolved large networks demonstrate very striking power laws, implying that there are far more small communities than large ones. This notion of far more small things than large ones constitutes a new fundamental way of thinking for community detection.
Defining Least Community as a Homogeneous Group in Complex Networks
Jiang, Bin
2015-01-01
This paper introduces a new concept of least community that is as homogeneous as a random graph, and develops a new community detection algorithm from the perspective of homogeneity or heterogeneity. Based on this concept, we adopt head/tail breaks - a newly developed classification scheme for data with a heavy-tailed distribution - and rely on edge betweenness given its heavy-tailed distribution to iteratively partition a network into many heterogeneous and homogeneous communities. Surprisingly, the derived communities for any self-organized and/or self-evolved large networks demonstrate very striking power laws, implying that there are far more small communities than large ones. This notion of far more small things than large ones constitutes a new fundamental way of thinking for community detection. Keywords: head/tail breaks, ht-index, scaling, k-means, natural breaks, and classification
HOMOGENIZATION, SYMMETRY, AND PERIODIZATION IN DIFFUSIVE RANDOM MEDIA
Alen Alexanderian; Muruhan Rathinam; Rouben Rostamian
2012-01-01
We present a systematic study of homogenization of diffusion in random media with emphasis on tile-based random microstructures.We give detailed examples of several such media starting from their physical descriptions,then construct the associated probability spaces and verify their ergodicity.After a discussion of material symmetries of random media,we derive criteria for the isotropy of the homogenized limits in tile-based structures.Furthermore,we study the periodization algorithm for the numerical approximation of the homogenized diffusion tensor and study the algorithm's rate of convergence.For one dimensional tile-based media,we prove a central limit result,giving a concrete rate of convergence for periodization.We also provide numerical evidence for a similar central limit behavior in the case of two dimensional tile-based structures.
Homogeneous solutions of minimal massive 3D gravity
Charyyev, Jumageldi; Deger, Nihat Sadik
2017-07-01
In this paper, we systematically construct simply transitive homogeneous spacetime solutions of the three-dimensional minimal massive gravity (MMG) model. In addition to those that have analogs in topologically massive gravity, such as warped AdS and p p waves, there are several solutions genuine to MMG. Among them, there is a stationary Lifshitz metric with the dynamical exponent z =-1 and an anisotropic Lifshitz solution where all coordinates scale differently. Moreover, we identify a homogeneous Kundt-type solution at the chiral point of the theory. We also show that in a particular limit of the physical parameters in which the Cotton tensor drops out from the MMG field equation, homogeneous solutions exist only at the merger point in the parameter space if they are not conformally flat.
Improved FFT-based Homogenization for Periodic Media
Vondřejc, Jaroslav
2014-01-01
Moulinec and Suquet introduced FFT-based homogenization in 1994, and twenty years later, their approach is still effective for evaluating the homogenized properties arising from the periodic cell problem. This paper builds on the author's (2013) variational reformulation approximated by trigonometric polynomials establishing two numerical schemes: Galerkin approximation (Ga) and a version with numerical integration (GaNi). The latter approach, fully equivalent to the original Moulinec-Suquet algorithm, was used to evaluate guaranteed upper-lower bounds on homogenized coefficients incorporating a closed-form double grid quadrature. Here, these concepts are employed for the Ga scheme. Despite Ga's higher computational requirements, it outperforms GaNi with improved guaranteed bounds together with more predictable and monotone behaviors for an increase in the number of grid points. Numerical integration heading to block-sparse linear systems is extended here to materials defined via high-resolution images in a w...
Delineating relative homogeneous G+C domains in DNA sequences.
Li, W
2001-10-03
The concept of homogeneity of G+C content is always relative and subjective. This point is emphasized and quantified in this paper using a simple example of one sequence segmented into two subsequences. Whether the sequence is homogeneous or not can be answered by whether the two-subsequence model describes the DNA sequence better than the one-sequence model. There are at least three equivalent ways of looking at the 1-to-2 segmentation: Jensen-Shannon divergence measure, log likelihood ratio test, and model selection using Bayesian information criterion. Once a criterion is chosen, a DNA sequence can be recursively segmented into multiple domains. We use one subjective criterion called segmentation strength based on the Bayesian information criterion. Whether or not a sequence is homogeneous and how many domains it has depend on this criterion. We compare six different genome sequences (yeast S. cerevisiae chromosome III and IV, bacterium M. pneumoniae, human major histocompatibility complex sequence, longest contigs in human chromosome 21 and 22) by recursive segmentations at different strength criteria. Results by recursive segmentation confirm that yeast chromosome IV is more homogeneous than yeast chromosome III, human chromosome 21 is more homogeneous than human chromosome 22, and bacterial genomes may not be homogeneous due to short segments with distinct base compositions. The recursive segmentation also provides a quantitative criterion for identifying isochores in human sequences. Some features of our recursive segmentation, such as the possibility of delineating domain borders accurately, are superior to those of the moving-window approach commonly used in such analyses.
Encapsulation of volatiles by homogenized partially-cross linked alginates.
Inguva, Pavan K; Ooi, Shing Ming; Desai, Parind M; Heng, Paul W S
2015-12-30
Cross-linked calcium alginate gels are too viscous to be efficaciously incorporated into spray dried formulations. Thus, viscosity reduction is essential to ensure the processability of calcium alginate gels to be sprayed. Viscosity reduction by high pressure homogenization can open new formulation possibilities. Presently, testing of microcapsule integrity is also limited because either single particle tests neglect collective particle behaviours in bulk or bulk testing methods are often associated with single compressions which may not fully characterize individual particle strengths. The aim of this study was sub-divided into three objectives. First objective was to evaluate the impact of high pressure homogenization on gel viscosity. Second objective was to explore the use of the homogenized gels with modified starch for microencapsulation by spray drying. The final objective was to develop a stamping system as microcapsule strength tester that can assess microcapsules in bulk and evaluate the impact of multiple compressions. Collectively, this study would lead towards developing a pressure-activated patch of microcapsules with encapsulated volatiles and the method to assess the patch efficacy. The alginate gels largely experienced an exponential decay in viscosity when homogenized. Furthermore, the homogenized gels were successfully incorporated in spray drying formulations for microencapsulation. The custom-designed microcapsule strength tester was successfully used and shown to possess the required sensitivity to discern batches of microcapsules containing volatiles to have different release profiles. Addition of homogenized gels strengthened the microcapsules only at high wall to core ratios with low mass-load alginate gels. High mass-load gels weaken the microcapsules, exhibiting a higher release at low stamping pressures and wrinkling on the microcapsules surface.
Numerical computing of elastic homogenized coefficients for periodic fibrous tissue
Roman S.
2009-06-01
Full Text Available The homogenization theory in linear elasticity is applied to a periodic array of cylindrical inclusions in rectangular pattern extending to infinity in the inclusions axial direction, such that the deformation of tissue along this last direction is negligible. In the plane of deformation, the homogenization scheme is based on the average strain energy whereas in the third direction it is based on the average normal stress along this direction. Namely, these average quantities have to be the same on a Repeating Unit Cell (RUC of heterogeneous and homogenized media when using a special form of boundary conditions forming by a periodic part and an affine part of displacement. It exists an infinity of RUCs generating the considered array. The computing procedure is tested with different choices of RUC to control that the results of the homogenization process are independent of the kind of RUC we employ. Then, the dependence of the homogenized coefficients on the microstructure can be studied. For instance, a special anisotropy and the role of the inclusion volume are investigated. In the second part of this work, mechanical traction tests are simulated. We consider two kinds of loading, applying a density of force or imposing a displacement. We test five samples of periodic array containing one, four, sixteen, sixty-four and one hundred of RUCs. The evolution of mean stresses, strains and energy with the numbers of inclusions is studied. Evolutions depend on the kind of loading, but not their limits, which could be predicted by simulating traction test of the homogenized medium.
Stochastic model of milk homogenization process using Markov's chain
A. A. Khvostov
2016-01-01
Full Text Available The process of development of a mathematical model of the process of homogenization of dairy products is considered in the work. The theory of Markov's chains was used in the development of the mathematical model, Markov's chain with discrete states and continuous parameter for which the homogenisation pressure is taken, being the basis for the model structure. Machine realization of the model is implemented in the medium of structural modeling MathWorks Simulink™. Identification of the model parameters was carried out by minimizing the standard deviation calculated from the experimental data for each fraction of dairy products fat phase. As the set of experimental data processing results of the micrographic images of fat globules of whole milk samples distribution which were subjected to homogenization at different pressures were used. Pattern Search method was used as optimization method with the Latin Hypercube search algorithm from Global Optimization Тoolbox library. The accuracy of calculations averaged over all fractions of 0.88% (the relative share of units, the maximum relative error was 3.7% with the homogenization pressure of 30 MPa, which may be due to the very abrupt change in properties from the original milk in the particle size distribution at the beginning of the homogenization process and the lack of experimental data at homogenization pressures of below the specified value. The mathematical model proposed allows to calculate the profile of volume and mass distribution of the fat phase (fat globules in the product, depending on the homogenization pressure and can be used in the laboratory and research of dairy products composition, as well as in the calculation, design and modeling of the process equipment of the dairy industry enterprises.
Absolute homogeneity test of Kelantan catchment precipitation series
Ros, Faizah Che; Tosaka, Hiroyuki; Sasaki, Kenji; Sidek, Lariyah Mohd; Basri, Hidayah
2015-05-01
Along the Kelantan River in north east of Malaysia Peninsular, there are several areas often damaged by flood during north-east monsoon season every year. It is vital to predict the expected behavior of precipitation and river runoff for reducing flood damages of the area under rapid urbanization and future planning. Nevertheless, the accuracy and reliability of any hydrological and climate studies vary based on the quality of the data used. The factors causing variations on these data are the method of gauging and data collection, stations environment, station relocation and the reliability of the measurement tool affect the homogenous precipitation records. Hence in this study, homogeneity of long precipitation data series is checked via the absolute homogeneity test consisting of four methods namely Pettitt test, standard normal homogeneity test (SNHT), Buishand range test and Von Neumann ratio test. For homogeneity test, the annual rainfall amount from the daily precipitation records at stations located in Kelantan operated by Department of Irrigation and Drainage Malaysia were considered in this study. The missing values were completed using the correlation and regression and inverse distance method. The data network consists of 103 precipitation gauging stations where 31 points are inactive, 6 gauging stations had missing precipitation values more than five years in a row and 16 stations have records less than twenty years. So total of 50 stations gauging stations were evaluated in this analysis. With the application of the mentioned methods and further graphical analysis, inhomogeneity was detected at 4 stations and 46 stations are found to be homogeneous.
Czank, Charles; Simmer, Karen; Hartmann, Peter E
2010-05-01
The combination of ultrasound and heat (thermoultrasound) is an emerging food preservation technique that retains higher quantities of bioactive components compared with current thermal pasteurization practice, but has not yet been assessed for pasteurizing human milk. Artificially contaminated human milk samples were treated with ultrasound (20 kHz, 150 watts) with and without heating. The retention of four human milk proteins was quantified by biochemical assay and laser scattering particle sizing was used to determine the extent of homogenization. While ultrasonic treatment was effective at inactivating Escherichia coli (D4 degrees C=5.94 min), Staphylococcus epidermidis exhibited resistance (D4 degrees C=16.01 min). Thermoultrasonic treatment was considerably more effective (Esch. coli D45 degrees C=1.74 min, D50 degrees C=0.89 min; Staph. epidermidis D45 degrees C=2.08 min, D50 degrees C=0.94 minutes) with a predicted retention (2.8 min treatment, 50 degrees C) of secretory IgA lysozyme, lactoferrin and bile salt stimulated lipase of 91, 80, 77, and 45%, respectively. Homogenization of the milk samples occurred after 5 min and 2 min of ultrasonic and thermoultrasonic treatment, respectively. Thermoultrasonic treatment is an effective method for pasteurizing donor human milk and retaining a greater proportion of bioactive components compared with current practices. However, further studies are required to assess the practicality of applying this technique routinely to donor human milk.
Homogeneous illusion device exhibiting transformed and shifted scattering effect
Mei, Jin-Shuo; Wu, Qun; Zhang, Kuang; He, Xun-Jun; Wang, Yue
2016-06-01
Based on the theory of transformation optics, a type of homogeneous illusion device exhibiting transformed and shifted scattering effect is proposed in this paper. The constitutive parameters of the proposed device are derived, and full-wave simulations are performed to validate the electromagnetic properties of transformed and shifted scattering effect. The simulation results show that the proposed device not only can visually shift the image of target in two dimensions, but also can visually transform the shape of target. It is expected that such homogeneous illusion device could possess potential applications in military camouflage and other field of electromagnetic engineering.
Load Balancing in a Networked Environment through Homogenization
Hossain, M Shahriar; Deb, Debzani; Khan, Kazi Muhammad Najmul Hasan; Joarder, Md Mahbubul Alam
2011-01-01
Distributed processing across a networked environment suffers from unpredictable behavior of speedup due to heterogeneous nature of the hardware and software in the remote machines. It is challenging to get a better performance from a distributed system by distributing task in an intelligent manner such that the heterogeneous nature of the system do not have any effect on the speedup ratio. This paper introduces homogenization, a technique that distributes and balances the workload in such a manner that the user gets the highest speedup possible from a distributed environment. Along with providing better performance, homogenization is totally transparent to the user and requires no interaction with the system.
Analysis of stability of a homogeneous state of anisotropic plasma
Zakharov, V. Yu., E-mail: vladiyuz@mail.ru; Chernova, T. G., E-mail: chernova-tg@yandex.ru; Stepanov, S. E., E-mail: stepanov@bmstu-kaluga.ru [Bauman Moscow State Technical University, Kaluga Branch (Russian Federation)
2015-04-15
Small-amplitude waves in collisionless magnetized plasma are considered in the framework of one-fluid anisotropic magnetohydrodynamics with allowance for the anisotropy of the pressure and thermal flux. Stability of a homogeneous plasma state is analyzed using an eighth-order dispersion relation. Restrictions on the parameters of the homogeneous state at which the dispersion relation has no complex roots at any value of the angle between the wave vector and the unperturbed magnetic field are obtained. The applied method also makes it possible to determine the types of unstable waves.
On the Mechanical Interaction of Light With Homogeneous Liquids
de Reus, Michiel
2013-01-01
We investigate one of the consequences of the three competing models describing the mechanical interaction of light with a dielectric medium. According to both the Abraham and Minkowski models the time-averaged force density is zero inside a homogeneous dielectric, whereas the induced-current Lorentz force model predicts a non-zero force density. We argue that the latter force, if exists, could drive a hydrodynamic flow inside a homogeneous fluid. Our numerical experiments show that such flows have distinct spatial patterns and may influence the dynamics of particles in a water-based single-beam optical trap.
Homogeneity studies in reference materials with Zeeman solid sampling GFAAS
Mohl, C.; Stoeppler, M.; Grobecker, K.H.
1987-09-01
The homogeneity of lead and cadmium in reference materials was investigated by solid sampling GFAAS. The following materials were comparatively analyzed: Standard reference materials from NBS, Washington 1567 wheat flour, 1568 rice flour and 1577a bovine liver, certified reference materials from BCR, Brussels, No 63 milk powder, No 184 bovine muscle, No 185 bovine liver, No 186 pig kidney, No 189 wholemeal flour, No 191 brown bread and a whole fish (dab) homogenate from the environmental specimen bank in the FRG. The results are remarkably different for the investigated materials.
Quasi-single-mode homogeneous 31-core fibre
Sasaki, Y.; Saitoh, S.; Amma, Y.
2015-01-01
A homogeneous 31-core fibre with a cladding diameter of 230 μm for quasi-single-mode transmission is designed and fabricated. LP01-crosstalk of -38.4 dB/11 km at 1550 nm is achieved by using few-mode trench-assisted cores.......A homogeneous 31-core fibre with a cladding diameter of 230 μm for quasi-single-mode transmission is designed and fabricated. LP01-crosstalk of -38.4 dB/11 km at 1550 nm is achieved by using few-mode trench-assisted cores....
Quaternionic-like manifolds and homogeneous twistor spaces.
Pantilie, Radu
2016-12-01
Motivated by the quaternionic geometry corresponding to the homogeneous complex manifolds endowed with (holomorphically) embedded spheres, we introduce and initiate the study of the 'quaternionic-like manifolds'. These contain, as particular subclasses, the CR quaternionic and the ρ-quaternionic manifolds. Moreover, the notion of 'heaven space' finds its adequate level of generality in this setting: (essentially) any real analytic quaternionic-like manifold admits a (germ) unique heaven space, which is a ρ-quaternionic manifold. We, also, give a natural construction of homogeneous complex manifolds endowed with embedded spheres, thus, emphasizing the abundance of the quaternionic-like manifolds.
Note on integrability of certain homogeneous Hamiltonian systems
Szumiński, Wojciech [Institute of Physics, University of Zielona Góra, Licealna 9, PL-65-407, Zielona Góra (Poland); Maciejewski, Andrzej J. [Institute of Astronomy, University of Zielona Góra, Licealna 9, PL-65-407, Zielona Góra (Poland); Przybylska, Maria, E-mail: M.Przybylska@if.uz.zgora.pl [Institute of Physics, University of Zielona Góra, Licealna 9, PL-65-407, Zielona Góra (Poland)
2015-12-04
In this paper we investigate a class of natural Hamiltonian systems with two degrees of freedom. The kinetic energy depends on coordinates but the system is homogeneous. Thanks to this property it admits, in a general case, a particular solution. Using this solution we derive necessary conditions for the integrability of such systems investigating differential Galois group of variational equations. - Highlights: • Necessary integrability conditions for some 2D homogeneous Hamilton systems are given. • Conditions are obtained analysing differential Galois group of variational equations. • New integrable and superintegrable systems are identified.
Ergodic theory and the duality principle on homogeneous spaces
Gorodnik, Alexander
2012-01-01
We prove mean and pointwise ergodic theorems for the action of a discrete lattice subgroup in a connected algebraic Lie group, on infinite volume homogeneous algebraic varieties. Under suitable necessary conditions, our results are quantitative, namely we establish rates of convergence in the mean and pointwise ergodic theorems, which can be estimated explicitly. Our results give a precise and in most cases optimal quantitative form to the duality principle governing dynamics on homogeneous spaces. We illustrate their scope in a variety of equidistribution problems.
Generalizing Fisher's “reproductive value”: Nonlinear, homogeneous, biparental systems*
Samuelson, Paul A.
1977-01-01
Biparental demographic models violate linearity. However, in their early “dilute” stages before limited environment resources bring need for competitive selection, first-degree-homogeneous relations obtain. For them, a reproductive-value function of the initial coordinates is defined to recapitulate their contribution to the asymptotically dominating mode of exponential growth: now the generalized Fisher reproductive value of one sex is altered by relative numbers of the other sex. The new reproductive-value function is also derived for general systems of homogeneous-first-degree differential and difference equations, and is shown to grow from the start at the asymptotic growth rate. PMID:16592475
Computational Homogenization of Fresh Concrete Flow Around Reinforcing Bars
Kolařík, Filip; Zeman, Jan
2016-01-01
Motivated by casting of fresh concrete in reinforced concrete structures, we introduce a numerical model of a steady-state non-Newtonian fluid flow through a porous domain. Our approach combines homogenization techniques to represent the reinforced domain by the Darcy law with an interface coupling of the Stokes and Darcy flows through the Beavers-Joseph-Saffman conditions. The ensuing two-scale problem is solved by the Finite Element Method with consistent linearization and the results obtained from the homogenization approach are verified against fully resolved direct numerical simulations.
LETTER TO THE EDITOR: Homogeneous nucleation for superheated crystal
Iwamatsu, Masao
1999-01-01
Homogeneous nucleation of liquid droplets in superheated crystals is considered in order to estimate the maximum superheating of crystals. Using the previously derived universal order parameter model of the crystal-melt transition (Iwamatsu M and Horii K 1996 J. Phys. Soc. Japan 65 2311), it is determined that the catastrophic homogeneous nucleation occurs at 0953-8984/11/1/001/img1, where 0953-8984/11/1/001/img2 is the equilibrium melting point. This numerical estimation is consistent with the results of maximum-superheating experiments.
Homogenization of long fiber reinforced composites including fiber bending effects
Poulios, Konstantinos; Niordson, Christian Frithiof
2016-01-01
This paper presents a homogenization method, which accounts for intrinsic size effects related to the fiber diameter in long fiber reinforced composite materials with two independent constitutive models for the matrix and fiber materials. A new choice of internal kinematic variables allows...... of the reinforcing fibers is captured by higher order strain terms, resulting in an accurate representation of the micro-mechanical behavior of the composite. Numerical examples show that the accuracy of the proposed model is very close to a non-homogenized finite-element model with an explicit discretization...
Massive gravity: nonlinear instability of the homogeneous and isotropic universe
De Felice, Antonio; Mukohyama, Shinji
2012-01-01
We study the propagating modes for nonlinear massive gravity on a Bianchi type--I manifold. We analyze their kinetic terms and dispersion relations as the background manifold approaches the homogeneous and isotropic limit. We show that in this limit, at least one ghost always exists and that its frequency tends to vanish for large scales, meaning that it cannot be integrated out from the low energy effective theory. Since this ghost mode can be considered as a leading nonlinear perturbation around a homogeneous and isotropic background, we conclude that the universe in this theory must be either inhomogeneous or anisotropic.
MICROBIOLOGICAL ASSAY FOR VITAMIN B
Bishnoi Kapil*, , ,; Kataria Mahesh; Singhal Vipin; Gupta Deepika
2012-01-01
Micronutrients added to foods are analyzed using various procedures depending on their nature and properties. The microbiological assays are better than chemical method because any suitable change in vitamin molecule which may not be detected by chemical method will be revealed by change in microbial activity. The microbiological assay of vitamins is based upon the comparison of the stimulation of growth of bacteria by measured concentration of vitamin with that produced by known concentratio...
Carina Ladeira
2015-06-01
The results concerning of positive findings by micronuclei and non significant ones by comet assay, are corroborated by Deng et al. (2005 study performed in workers occupationally exposed to methotrexate, also a cytostatic drug. According to Cavallo et al. (2009, the comet assay seems to be more suitable for the prompt evaluation of the genotoxic effects, for instance, of polycyclic aromatic hydrocarbons mixtures containing volatile substances, whereas the micronucleus test seems more appropriate to evaluate the effects of exposure to antineoplastic agents. However, there are studies that observed an increase in both the comet assay and the micronucleus test in nurses handling antineoplastic drugs, although statistical significance was only seen in the comet assay, quite the opposite of our results (Maluf & Erdtmann, 2000; Laffon et al. 2005.
High deposition rate nanocrystalline silicon with enhanced homogeneity
Verkerk, A.; Rath, J.K.; Schropp, R.E.I.
2010-01-01
High rate growth of hydrogenated nanocrystalline silicon (nc-Si:H) brings additional challenges for the homogeneity in the growth direction, since the start-up effects affect a larger portion of the film, and the very high degree of depletion increases the influence of back diffusion from the inacti
Political homogeneity can nurture threats to research validity.
Chambers, John R; Schlenker, Barry R
2015-01-01
Political homogeneity within a scientific field nurtures threats to the validity of many research conclusions by allowing ideologically compatible values to influence interpretations, by minimizing skepticism, and by creating premature consensus. Although validity threats can crop in any research, the usual corrective activities in science are more likely to be minimized and delayed.
Homogeneous Hyperbolic Systems for Terahertz and Far-Infrared Frequencies
Leonid V. Alekseyev
2012-01-01
Full Text Available We demonstrate that homogeneous naturally-occurring materials can form hyperbolic media, and can be used for nonmagnetic negative refractive index systems. We present specific realizations of the proposed approach for the THz and far-IR frequencies. The proposed structures operate away from resonance, thereby promising the capacity for low-loss devices.
Are Heterogeneous or Homogeneous Groups More Beneficial to Students?
Schullery, Nancy M.; Schullery, Stephen E.
2006-01-01
This study investigated the relative benefits to the student of working in homogeneous versus heterogeneous classroom groups. Correlation analysis of 18 desirable outcomes versus 8 personality-based heterogeneity variables reveals that heterogeneity associates with advantages as well as disadvantages. Ways in which group composition might be…
On the $psi$-dichotomy for homogeneous linear differential equations
Pham Ngoc Boi
2006-03-01
Full Text Available In this article we present some conditions for the $psi$-dichotomy of the homogeneous linear differential equation $x'=A(tx$. Under our condition every $psi$-integrally bounded function $f$ the nonhomogeneous linear differential equation $x'=A(tx +f(t$ has at least one $psi$-bounded solution on $(0,+infty$.
ON A SET OF h-HARMONIC HOMOGENEOUS POLYNOMIALS
Wang Renhong; Zhou Heng
2003-01-01
In this paper, we study the homogeneous polynomials orthogonal with the weight function h (x(d))= x2k11gree n. It is simpler than the formula in [2], and can be regarded as an extension of [1] under the weighted case.
On the transition between heterogeneous and homogeneous freezing
K. Gierens
2002-12-01
Full Text Available Box model simulations of an uplifting and adiabatically cooling cloud of aerosol have been performed in order to study the transition between cirrus formation dominated by homogeneous nucleation of ice to that dominated by heterogeneous nucleation. The aerosol was assumed to consist of an internal mixture of sulfuric acid solution droplets with inclusions of soot. The simulations show that the transition from heterogeneous to homogeneous nucleation occurs over a narrow range of soot concentration. Thus it seems to be possible to fix critical concentrations of heterogeneous ice nuclei which must be exceeded if heterogeneous freezing dominates cirrus formation. A formula has been derived that allows to compute the critical concentrations of heterogeneous ice nuclei as a function of temperature, updraft speed, ambient pressure, and supersaturation at which heterogeneous freezing occurs. Generally, homogeneous nucleation dominates in regions with updrafts stronger than 20 cmps, with the exception of heavily polluted areas which could be common in the northern hemisphere due to air traffic, where updrafts of the order 1 mps may be necessary to render heterogeneous nucleation unimportant. According to the present results it cannot be excluded that heterogeneous nucleation plays a more important role for cirrus formation in the northern midlatitudes than anywhere else. A possible consequence of these results is that air pollution may lead to a higher coverage of cirrus clouds, but then these clouds will be optically thinner than clouds formed by homogeneous freezing, with the exception of regions where condensation trails are frequent.
On Euler's Theorem for Homogeneous Functions and Proofs Thereof.
Tykodi, R. J.
1982-01-01
Euler's theorem for homogenous functions is useful when developing thermodynamic distinction between extensive and intensive variables of state and when deriving the Gibbs-Duhem relation. Discusses Euler's theorem and thermodynamic applications. Includes six-step instructional strategy for introducing the material to students. (Author/JN)
Homogeneity of Moral Judgment? Apprentices Solving Business Conflicts.
Beck, Klaus; Heinrichs, Karin; Minnameier, Gerhard; Parche-Kawik, Kirsten
In an ongoing longitudinal study that started in 1994, the moral development of business apprentices is being studied. The focal point of this project is a critical analysis of L. Kohlberg's thesis of homogeneity, according to which people should judge every moral issue from the point of view of their "modal" stage (the most frequently…
Homogenization of intergranular fracture towards a transient gradient damage model
Sun, G.; Poh, L. H.
2016-10-01
This paper focuses on the intergranular fracture of polycrystalline materials, where a detailed model at the meso-scale is translated onto the macro-level through a proposed homogenization theory. The bottom-up strategy involves the introduction of an additional macro-kinematic field to characterize the average displacement jump within the unit cell. Together with the standard macro-strain field, the underlying processes are propagated onto the macro-scale by imposing the equivalence of power and energy at the two scales. The set of macro-governing equations and constitutive relations are next extracted naturally as per standard thermodynamics procedure. The resulting homogenized microforce balance recovers the so-called 'implicit' gradient expression with a transient nonlocal interaction. The homogenized gradient damage model is shown to fully regularize the softening behavior, i.e. the structural response is made mesh-independent, with the damage strain correctly localizing into a macroscopic crack, hence resolving the spurious damage growth observed in many conventional gradient damage models. Furthermore, the predictive capability of the homogenized model is demonstrated by benchmarking its solutions against reference meso-solutions, where a good match is obtained with minimal calibrations, for two different grain sizes.
Homogeneous vs. heterogeneous nucleation in water-dicarboxylic acid systems
A. I. Hienola
2008-10-01
Full Text Available Binary heterogeneous nucleation of water-succinic/glutaric/malonic/adipic acid on nanometer-sized particles is investigated within the frame of classical heterogeneous nucleation theory. Homogeneous nucleation is also included for comparison. It is found that the nucleation probabilities depend on the contact angle and on the size of the seed particles. New thermodynamical properties, such as saturation vapor pressure, density and surface tension for all the dicarboxylic acid aqueous solutions are included in the calculations. While the new surface tension and density formulations do not bring any significant difference in the computed nucleation rate for homogeneous nucleation for succinic and glutaric acids, the use of the newly derived equations for the vapor pressure decrease the acid concentrations in gas phase with 3 orders of magnitude. According to our calculations, the binary heterogeneous nucleation of succinic acid-water and glutaric acid-water – although it requires a 3–4 orders of magnitude lower vapor concentrations than the homogeneous nucleation – cannot take place in atmospheric conditions. On the other hand binary homogeneous nucleation of adipic acid-water systems might be possible in conditions occuring in upper boundary layer. However, a more detailed characterization of the interaction between the surface and the molecules of the nucleating vapor should be considered in the future.
KINETIC THEORY OF PLASMA WAVES: Part II: Homogeneous Plasma
Westerhof, E.
2010-01-01
The theory of electromagnetic waves in a homogeneous plasma is reviewed. The linear response of the plasma to the waves is obtained in the form of the dielectric tensor. Waves ranging from the low frequency Alfven to the high frequency electron cyclotron waves are discussed in the limit of the cold
Kinetic theory of plasma waves: Part II homogeneous plasma
Westerhof, E.
2000-01-01
The theory of electromagnetic waves in a homogeneous plasma is reviewed. The linear response of the plasma to the waves is obtained in the form of the dielectric tensor. Waves ranging from the low frequency Alfven to the high frequency electron cyclotron waves are discussed in the limit of the cold
Kinetic theory of plasma waves - Part II: Homogeneous plasma
Westerhof, E.
2008-01-01
The theory of electromagnetic waves in a homogeneous plasma is reviewed. The linear response of the plasma to the waves is obtained in the form of the dielectric tensor. Waves ranging from the low frequency Alfven to the high frequency electron cyclotron waves axe discussed in the limit of the cold
Tandem fluid queues fed by homogeneous on-off sources
Scheinhardt, Willem R.W.; Aalto, Samuli
2000-01-01
We consider a tandem fluid model with multiple consecutive buffers. The input of buffer j+1 is the output from buffer j, while the first buffer is fed by a, possibly infinite, number of independent homogeneous on–off sources. The sources have exponentially distributed silent periods and generally di
Non-Homogeneous Riemann Boundary Value Problem with Radicals
无
2002-01-01
The solution of the non-homogeneous Riemann boundary value problem with radicals (1.2)together with its condition of solvability is obtained for arbitrary positive integers p and q, which generalizes the results for the case p=q=2.
Heterogeneous and homogeneous reactions of pyrolysis vapors from pine wood
Hoekstra, E.; Westerhof, R.J.M.; Brilman, D.W.F.; Swaaij, van W.P.M.; Kersten, S.R.A.; Hogendoorn, J.A.; Windt, M.
2012-01-01
To maximize oil yields in the fast pyrolysis of biomass it is generally accepted that vapors need to be rapidly quenched. The influence of the heterogeneous and homogeneous vapor-phase reactions on yields and oil composition were studied using a fluidized-bed reactor. Even high concentrations of min
Simple compactifications and polar decomposition of homogeneous real spherical spaces
Knop, Friedrich; Krötz, Bernhard; Sayag, Eitan
2015-01-01
Let Z be an algebraic homogeneous space Z = G/H attached to real reductive Lie group G. We assume that Z is real spherical, i.e., minimal parabolic subgroups have open orbits on Z. For such spaces, we investigate their large scale geometry and provide a polar decomposition. This is obtained from...
Kinetic theory of plasma waves: Part II homogeneous plasma
Westerhof, E.
2000-01-01
The theory of electromagnetic waves in a homogeneous plasma is reviewed. The linear response of the plasma to the waves is obtained in the form of the dielectric tensor. Waves ranging from the low frequency Alfven to the high frequency electron cyclotron waves are discussed in the limit of the cold
KINETIC THEORY OF PLASMA WAVES: Part II: Homogeneous Plasma
Westerhof, E.
2010-01-01
The theory of electromagnetic waves in a homogeneous plasma is reviewed. The linear response of the plasma to the waves is obtained in the form of the dielectric tensor. Waves ranging from the low frequency Alfven to the high frequency electron cyclotron waves are discussed in the limit of the cold
Kinetic theory of plasma waves - Part II: Homogeneous plasma
Westerhof, E.
2008-01-01
The theory of electromagnetic waves in a homogeneous plasma is reviewed. The linear response of the plasma to the waves is obtained in the form of the dielectric tensor. Waves ranging from the low frequency Alfven to the high frequency electron cyclotron waves axe discussed in the limit of the cold
EPR spectroscopy as a tool in homogeneous catalysis research
Goswami, M.; Chirila, A.; Rebreyend, C.; de Bruin, B.
2015-01-01
In the context of homogeneous catalysis, open-shell systems are often quite challenging to characterize. Nuclear magnetic resonance (NMR) spectroscopy is the most frequently applied tool to characterize organometallic compounds, but NMR spectra are usually broad, difficult to interpret and often fut
Change in Homogeneous and Heterogeneous Sensitivity Training Groups
Pollack, Herbert B.
1971-01-01
Among the findings was that the heterogeneous groups showed more change on the FIRO scale. Also, on rating scales measuring positive feeling towards group, the initial superiority of homogeneous groups was reduced by the end of the T group and that heterogeneous groups manifested more significant increases. (Author/CG)
Missing data analysis and homogeneity test for Turkish precipitation series
Mahmut Firat; Fatih Dikbas; A Cem Koç; Mahmud Gungor
2010-12-01
In this study, missing value analysis and homogeneity tests were conducted for 267 precipitation stations throughout Turkey. For this purpose, the monthly and annual total precipitation records at stations operated by Turkish State Meteorological Service (DMI) from 1968 to 1998 were considered. In these stations, precipitation records for each month was investigated separately and the stations with missing values for too many years were eliminated. The missing values of the stations were completed by Expectation Maximization (EM) method by using the precipitation records of the nearest gauging station. In this analysis, 38 stations were eliminated because they had missing values for more than 5 years, 161 stations had no missing values and missing precipitation values were completed in the remaining 68 stations. By this analysis, annual total precipitation data were obtained by using the monthly values. These data should be hydrologically and statistically reliable for later hydrological, meteorological, climate change modelling and forecasting studies. For this reason, Standard Normal Homogeneity Test (SNHT), (Swed–Eisenhart) Runs Test and Pettitt homogeneity tests were applied for the annual total precipitation data at 229 gauging stations from 1968 to 1998. The results of each of the testing methods were evaluated separately at a signiﬁcance level of 95% and the inhomogeneous years were determined. With the application of the aforementioned methods, inhomogeneity was detected at 50 stations of which the natural structure was deteriorated and 179 stations were found to be homogeneous.
IMPACT OF COOKING HOMOGENEITY ON PULP STRENGTH PROPERTIES
Bofeng Mao
2004-01-01
Cooking homogeneity of kraft pulping can have significant impact on pulp strength properties. We have studied cooking homogeneity of two kraft digesters within Stora Enso. One of the digesters is a one-vessel hydraulic continuous digester (digester A)and the other one is a two-vessel vapor/liquor phase continuous digester (digester B). They are located in the same mill and use the same softwood chip quality. Due to the differences in chip pre-steaming and cooking facilities and conditions, significant differences in pulp strength properties, reject contents and kappa variations have been found between the pulps produced in the two digesters. Digester A has a modem chip bin with efficient pre-heating and air removal, whereas digester B has poor chip pre-steaming conditions. Our strength delivery studies show that although the two digesters produce pulps with the same kappa number and viscosity, the pulp produced in digester A has about 20% higher strength delivery, l%-unit (based on wood) lower reject content and lower kappa variations based on FTIR spectra analysis on pulp sheet than the pulp produced in digester B.Results of the studies indicate that more homogeneous cooking, i.e., lower lignin/kappa variation in the fibers, leads to improved pulp strength properties. Efficient chip pre-steaming is important/essential for achieving homogeneous cooking. Rebuilding the equipment for improving chip pre-steaming conditions of digester B is to be carried out.
Thick Plate Homogenization of Sandwich Panels Including Folded Cellular Cores
LEBEE, Arthur; Sab, Karam
2011-01-01
In the present work, we provide the Bending-Gradient homogenization scheme and apply it to a sandwich panel including the chevron pattern. It turns out that the shear forces stiffness of the sandwich panel is strongly influenced by a skin distortion phenomenon which cannot be neglected in conventional design.
Short wave stability of homogeneous shear flows with variable topography
窦华书; V. GANESH
2014-01-01
For the stability problem of homogeneous shear flows in sea straits of arbitrary cross section, a sufficient condition for stability is derived under the condition of inviscid flow. It is shown that there is a critical wave number, and if the wave number of a normal mode is greater than this critical wave number, the mode is stable.
Matrix algorithms for solving (in)homogeneous bound state equations.
Blank, M; Krassnigg, A
2011-07-01
In the functional approach to quantum chromodynamics, the properties of hadronic bound states are accessible via covariant integral equations, e.g. the Bethe-Salpeter equation for mesons. In particular, one has to deal with linear, homogeneous integral equations which, in sophisticated model setups, use numerical representations of the solutions of other integral equations as part of their input. Analogously, inhomogeneous equations can be constructed to obtain off-shell information in addition to bound-state masses and other properties obtained from the covariant analogue to a wave function of the bound state. These can be solved very efficiently using well-known matrix algorithms for eigenvalues (in the homogeneous case) and the solution of linear systems (in the inhomogeneous case). We demonstrate this by solving the homogeneous and inhomogeneous Bethe-Salpeter equations and find, e.g. that for the calculation of the mass spectrum it is as efficient or even advantageous to use the inhomogeneous equation as compared to the homogeneous. This is valuable insight, in particular for the study of baryons in a three-quark setup and more involved systems.
Detecting the violation of variance homogeneity in mixed models.
Fang, Xicheng; Li, Jialiang; Wong, Weng Kee; Fu, Bo
2016-12-01
Mixed-effects models are increasingly used in many areas of applied science. Despite their popularity, there is virtually no systematic approach for examining the homogeneity of the random-effects covariance structure commonly assumed for such models. We propose two tests for evaluating the homogeneity of the covariance structure assumption across subjects: one is based on the covariance matrices computed from the fitted model and the other is based on the empirical variation computed from the estimated random effects. We used simulation studies to compare performances of the two tests for detecting violations of the homogeneity assumption in the mixed-effects models and showed that they were able to identify abnormal clusters of subjects with dissimilar random-effects covariance structures; in particular, their removal from the fitted model might change the signs and the magnitudes of important predictors in the analysis. In a case study, we applied our proposed tests to a longitudinal cohort study of rheumatoid arthritis patients and compared their abilities to ascertain whether the assumption of covariance homogeneity for subject-specific random effects holds. © The Author(s) 2014.
RELAXATION OF FUNCTIONALS INVOLVING HOMOGENEOUS FUNCTIONS AND INVARIANCE OF ENVELOPES
无
2002-01-01
The authors compute the quasiconvex envelope of certain functions defined on the space Mmn of real m× n matrices via a homogeneous function on Mmn. They also deduce invariance properties for various convex envelopes from corresponding invariance properties satisfied by a function. Some applications related in particular to nonlinear elasticity are given.
A micromorphic computational homogenization framework for heterogeneous materials
Biswas, R.; Poh, L. H.
2017-05-01
The conventional first-order computational homogenization framework is restricted to problems where the macro characteristic length scale is much larger than the underlying Representative Volume Element (RVE). In the absence of a clear separation of length scales, higher-order enrichment is required to capture the influence of the underlying rapid fluctuations, otherwise neglected in the first-order framework. In this contribution, focusing on matrix-inclusion composites, a novel computational homogenization framework is proposed such that standard continuum models at the micro-scale translate onto the macro-scale to recover a micromorphic continuum. Departing from the conventional FE2 framework where a macroscopic strain tensor characterizes the average deformation within the RVE, our formulation introduces an additional macro kinematic field to characterize the average strain in the inclusions. The two macro kinematic fields, each characterizing a particular aspect of deformation within the RVE, thus provide critical information on the underlying rapid fluctuations. The net effect of these fluctuations, as well as the interactions between RVEs, are next incorporated naturally into the macroscopic virtual power statement through the Hill-Mandel condition. The excellent predictive capability of the proposed homogenization framework is illustrated through three benchmark examples. It is shown that the homogenized micromorphic model adequately captures the material responses, even in the absence of a clear separation of length scales between macro and micro.
Nickel N-heterocyclic carbene complexes in homogeneous catalysis
Berding, Joris
2009-01-01
Described in this thesis are the investigations into the chemistry of N-heterocyclic carbene (NHC) ligands and transition-metal complexes thereof. Specifically, a variety of N-heterocyclic carbene complexes of nickel were prepared, characterized and used as catalysts in three types of homogeneous ca
A variational approach to homogeneous scalar fields in General Relativity
Giambo', R; Magli, G
2006-01-01
A result of existence of homogeneous scalar field solutions between prescribed configurations is given, using a modified version of Euler--Maupertuis least action variational principle. Solutions are obtained as limit of approximating variational problems, solved using techniques introduced by Rabinowitz.
Modes of Homogeneous Barrier Discharge at Atmospheric Pressure in Helium
WANG Yan-Hui; WANG De-Zhen
2004-01-01
@@ The discharge modes of a homogeneous barrier discharge at atmospheric pressure in helium are investigated with a one-dimensional fluid model It is found that, either in single peak discharge or in multipeak discharge, there are two discharge modes: glow and Townsend modes. The structure and features of the two modes are compared.The conditions forming the two modes are discussed.
Lp-boundedness of flag kernels on homogeneous groups
Glowacki, Pawel
2010-01-01
We prove that the flag kernel singular integral operators of Nagel-Ricci-Stein on a homogeneous group are bounded on the Lp spaces. The gradation associated with the kernels is the natural gradation of the underlying Lie algebra. Our main tools are the Littlewood-Paley theory and a symbolic calculus combined in the spirit of Duoandikoetxea and Rubio de Francia.
From homogeneous eigenvalue problems to two-sex population dynamics.
Thieme, Horst R
2017-03-08
Enclosure theorems are derived for homogeneous bounded order-preserving operators and illustrated for operators involving pair-formation functions introduced by Karl-Peter Hadeler in the late 1980s. They are applied to a basic discrete-time two-sex population model and to the relation between the basic turnover number and the basic reproduction number.
On Homogeneous Production Functions with Proportional Marginal Rate of Substitution
Alina Daniela Vîlcu
2013-01-01
Full Text Available We completely classify homogeneous production functions with proportional marginal rate of substitution and with constant elasticity of labor and capital, respectively. These classifications generalize some recent results of C. A. Ioan and G. Ioan (2011 concerning the sum production function.
Simulation of homogeneous crystal nucleation close to coexistence
Wolde, P.R. ten; Ruiz-Montero, M.J.; Frenkel, D.
1997-01-01
We discuss a numerical scheme to study homogeneous crystal nucleation. Using this approach, it is possible to compute the height of the free energy barrier that separates the solid from the liquid phase and the rate at which this barrier is crossed. We point out that there is a fundamental differenc
Applying homogeneous catalysis for the synthesis of pharmaceuticals.
Beller, M
2006-01-01
This article describes recent achievements of my research group in the Leibniz-Institut für Katalyse e.V. in the area of applied homogeneous catalysis for the synthesis of biologically active compounds. Special focus is given on the development of novel and practical palladium and copper catalysts for the functionalization of haloarenes and haloheteroarenes.
Synergy between experimental and computational approaches to homogeneous photoredox catalysis.
Demissie, Taye B; Hansen, Jørn H
2016-07-01
In this Frontiers article, we highlight how state-of-the-art density functional theory calculations can contribute to the field of homogeneous photoredox catalysis. We discuss challenges in the fields and potential solutions to be found at the interface between theory and experiment. The exciting opportunities and insights that can arise through such an interdisciplinary approach are highlighted.
Biotic homogenization of three insect groups due to urbanization.
Knop, Eva
2016-01-01
Cities are growing rapidly, thereby expected to cause a large-scale global biotic homogenization. Evidence for the homogenization hypothesis is mostly derived from plants and birds, whereas arthropods have so far been neglected. Here, I tested the homogenization hypothesis with three insect indicator groups, namely true bugs, leafhoppers, and beetles. In particular, I was interested whether insect species community composition differs between urban and rural areas, whether they are more similar between cities than between rural areas, and whether the found pattern is explained by true species turnover, species diversity gradients and geographic distance, by non-native or specialist species, respectively. I analyzed insect species communities sampled on birch trees in a total of six Swiss cities and six rural areas nearby. In all indicator groups, urban and rural community composition was significantly dissimilar due to native species turnover. Further, for bug and leafhopper communities, I found evidence for large-scale homogenization due to urbanization, which was driven by reduced species turnover of specialist species in cities. Species turnover of beetle communities was similar between cities and rural areas. Interestingly, when specialist species of beetles were excluded from the analyses, cities were more dissimilar than rural areas, suggesting biotic differentiation of beetle communities in cities. Non-native species did not affect species turnover of the insect groups. However, given non-native arthropod species are increasing rapidly, their homogenizing effect might be detected more often in future. Overall, the results show that urbanization has a negative large-scale impact on the diversity specialist species of the investigated insect groups. Specific measures in cities targeted at increasing the persistence of specialist species typical for the respective biogeographic region could help to stop the loss of biodiversity.
A Rapid Zika Diagnostic Assay to Measure Neutralizing Antibodies in Patients
Chao Shan
2017-03-01
Full Text Available The potential association of microcephaly and other congenital abnormalities with Zika virus (ZIKV infection during pregnancy underlines the critical need for a rapid and accurate diagnosis. Due to the short duration of ZIKV viremia in infected patients, a serologic assay that detects antibody responses to viral infection plays an essential role in diagnosing patient specimens. The current serologic diagnosis of ZIKV infection relies heavily on the labor-intensive Plaque Reduction Neutralization Test (PRNT that requires more than one-week turnaround time and represents a major bottleneck for patient diagnosis. To overcome this limitation, we have developed a high-throughput assay for ZIKV and dengue virus (DENV diagnosis that can attain the “gold standard” of the current PRNT assay. The new assay is homogeneous and utilizes luciferase viruses to quantify the neutralizing antibody titers in a 96-well format. Using 91 human specimens, we showed that the reporter diagnostic assay has a higher dynamic range and maintains the relative specificity of the traditional PRNT assay. Besides the improvement of assay throughput, the reporter virus technology has also shortened the turnaround time to less than two days. Collectively, our results suggest that, along with the viral RT-PCR assay, the reporter virus-based serologic assay could be potentially used as the first-line test for clinical diagnosis of ZIKV infection as well as for vaccine clinical trials.
Pathogenicty and immune prophylaxis of cag pathogenicity island gene knockout homogenic mutants
Huan-Jian Lin; Jing Xue; Yang Bai; Ji-De Wang; Ya-Li Zhang; Dian-Yuan Zhou
2004-01-01
AIM: To clarify the role of cag pathogenicity island (cagPAI)of Helicobacter pylori(H pylori) in the pathogenicity and immune prophylaxis of H pyloriinfection.METHODS: Three pairs of H pylori including 3 strains of cagPAI positive wildtype bacteria and their cagPAI knockout homogenic mutants were utilized. H pylori binding to the gastric epithelial cells was analyzed by flow cytometry assays.Apoptosis of gastric epithelial cells induced by H pylori was determined by ELISA assay. Prophylaxis effect of the wildtype and mutant strains was compared by immunization with the sonicate of the bacteria into mice model.RESULTS: No difference was found in the apoptasis between cagPAI positive and knockout H pylori strains in respective of the ability in the binding to gastric epithelial cells as well as the induction of apoptosis. Both types of the bacteria were able to protect the mice from the infection of H pylori after immunization, with no difference between them regarding to the protection rate as well as the stimulation of the proliferation of splenocytes of the mice.CONCLUSION: The role of cagPAI in the pathogenicity and prophylaxis of H pylori infection remains to be cleared.
Ma, Haiching; Horiuchi, Kurumi Y; Wang, Yuan; Kucharewicz, Stefan A; Diamond, Scott L
2005-04-01
Microfluidic technologies offer the potential for highly productive and low-cost ultra-high throughput screening and high throughput selectivity profiling. Such technologies need to provide the flexibility of plate-based assays as well as be less expensive to operate. Presented here is a unique microarray system (the Reaction Biology [Malvern, PA] DiscoveryDot), which runs over 6,000 homogeneous reactions per 1" x 3" microarray using chemical libraries or compound dilutions printed in 1-nl volumes. A simple and rapid piezo-activation method delivers from 30 to 300 pl of biochemical targets and detector chemistries to each reaction. The fluorescent signals are detected and analyzed with conventional microarray scanners and software. The DiscoveryDot platform is highly customizable, and reduces consumption of targets and reaction chemistries by >40-fold and the consumption of compounds by >10,000-fold, compared to 384-well plate assay. We demonstrate here that the DiscoveryDot platform is compatible with conventional large-volume well-based reactions, with a Z' factor of >0.6 for many enzymes, such as the caspase family enzymes, matrix metalloproteinase, serine proteases, kinases, and histone deacetylases. The platform is well equipped for 50% inhibitory concentration (IC50) profiling studies of enzyme inhibitors, with up to 10 dilution conditions of each test compound printed in duplicate, and each microarray chip can generate over 300 IC50 measurements against a given target.
Li, Yong; Zhao, Xiuhua; Zu, Yuangang; Zhang, Yin
2015-07-25
The aim of this study was to develop an alternative, more bio-available, better tolerated paclitaxel nanosuspension (PTXNS) for intravenous injection in comparison with commercially available Taxol(®) formulation. In this study, PTXNS was prepared by emulsification method through combination of high speed homogenizer and high pressure homogenization, followed by lyophilization process for intravenous administration. The main production parameters including volume ratio of organic phase in water and organic phase (Vo:Vw+o), concentration of PTX, content of PTX and emulsification time (Et), homogenization pressure (HP) and passes (Ps) for high pressure homogenization were optimized and their effects on mean particle size (MPS) and particle size distribution (PSD) of PTXNS were investigated. The characteristics of PTXNS, such as, surface morphology, physical status of paclitaxel (PTX) in PTXNS, redispersibility of PTXNS in purified water, in vitro dissolution study and bioavailability in vivo were all investigated. The PTXNS obtained under optimum conditions had an MPS of 186.8 nm and a zeta potential (ZP) of -6.87 mV. The PTX content in PTXNS was approximately 3.42%. Moreover, the residual amount of chloroform was lower than the International Conference on Harmonization limit (60 ppm) for solvents. The dissolution study indicated PTXNS had merits including effect to fast at the side of raw PTX and sustained-dissolution character compared with Taxol(®) formulation. Moreover, the bioavailability of PTXNS increased 14.38 and 3.51 times respectively compared with raw PTX and Taxol(®) formulation.
Rosas, Juan G; Blanco, Marcelo
2012-11-01
This article is the second of a series of two articles detailing the application of mixing index to assess homogeneity distribution in oral pharmaceutical solid dosage forms by image analysis. Chemical imaging (CI) is an emerging technique integrating conventional imaging and spectroscopic techniques with a view to obtaining spatial and spectral information from a sample. Near infrared chemical imaging (NIR-CI) has proved an excellent analytical tool for extracting high-quality information from sample surfaces. The primary objective of this second part was to demonstrate that the approach developed in the first part could be successfully applied to near infrared hyperspectral images of oral pharmaceutical solid dosage forms such as coated, uncoated and effervescent tablets, as well as to powder blends. To this end, we assessed a new criterion for establishing mixing homogeneity by using four different methods based on a three-dimensional (M×N×λ) data array of hyperspectral images (spectral standard deviations and correlation coefficients) or a two-dimensional (M×N) data array (concentration maps and binary images). The four methods were used applying macropixel analysis to the Poole (M(P)) and homogeneity (H%(Poole)) indices. Both indices proved useful for assessing the degree of homogeneity of pharmaceutical samples. The results testify that the proposed approach can be effectively used in the pharmaceutical industry, in the finished products (e.g., tablets) and in mixing unit operations for example, as a process analytical technology tool for the blending monitoring (see part 1).
Zhang, Zhihao; Xue, Jie; Jiang, Yanbin
2017-07-01
A method to improve the homogeneities of microstructure and mechanical property in an Al-Zn-Mg-Cu extruded product, which changes cooling rate after homogenization treatment to obtain the different distribution characteristics of the precipitates, was proposed and the microstructure evolution and mechanical properties of the alloy were investigated. The results show that the precipitates in the water-quenched billet are of mainly coarse particles with the content of about 2.0%, while a large number of needle-shaped precipitates are dispersively distributed in the furnace-cooled billet with the content of about 9.8%. Numerous precipitates distributed dispersively can improve the homogeneities of microstructure and mechanical property of the alloy during extrusion. For example, when the water-quenched billets are extruded at 390 and 430 °C followed by solution-aging treatment, the average grain sizes of the two bars are 3.4 and 8.1 μm, and the elongations to failure are 7.0 and 9.2%, respectively. When the furnace-cooled billets are extruded at 390 and 430 °C followed by solution-aging treatment, the average grain sizes of the two bars are 3.1 and 3.5 μm, respectively, and the elongations are basically the same, indicating the better microstructure homogeneity and mechanical properties.
Barcoded microchips for biomolecular assays.
Zhang, Yi; Sun, Jiashu; Zou, Yu; Chen, Wenwen; Zhang, Wei; Xi, Jianzhong Jeff; Jiang, Xingyu
2015-01-20
Multiplexed assay of analytes is of great importance for clinical diagnostics and other analytical applications. Barcode-based bioassays with the ability to encode and decode may realize this goal in a straightforward and consistent manner. We present here a microfluidic barcoded chip containing several sets of microchannels with different widths, imitating the commonly used barcode. A single barcoded microchip can carry out tens of individual protein/nucleic acid assays (encode) and immediately yield all assay results by a portable barcode reader or a smartphone (decode). The applicability of a barcoded microchip is demonstrated by human immunodeficiency virus (HIV) immunoassays for simultaneous detection of three targets (anti-gp41 antibody, anti-gp120 antibody, and anti-gp36 antibody) from six human serum samples. We can also determine seven pathogen-specific oligonucleotides by a single chip containing both positive and negative controls.
Radioreceptor assay method for insulin
Mori, K.F.; Wood, R.J. (Bureau of Drug Research, Health and Welfare Canada, Ottawa, Ontario. Health Protection Branch)
1984-01-01
A sensitive practical radioreceptor assay method for pharmaceutical insulin products has been developed with partially purified rat liver plasma membranes and the optimal conditions under which the best overall assay performance is obtainable have been defined. Intra- and inter-assay variations of the method averaged 7.3 and 12.2% of the man, respectively, when expressed as the coefficient of variation. Potency estimates of an insulin product obtained with the proposed method correlated well with those determined by the mouse convulsion bioassay method. Liver membranes prepared according to the method could be stored for up to ten weeks at 4/sup 0/C and for 6 months or more at -18/sup 0/C without losing insulin-binding ability.
Karsai, T; Elödi, P
1979-01-01
A chromatographic-videodensitometric assay was found to be appropriate for measuring the activity of glutamate dehydrogenase, aspartate aminotransferase, alanine aminotransferase, ornithine-2-oxoacid aminotransferase and histidine ammonia-lyase in human tissue homogenates. From the assay mixtures containing substrate(s), cofactor(s), buffer and tissue extract, five or ten microliters samples were taken at different time intervals and chromatographed on Dowex 50 X 8 type resin-coated chromatosheets. On each chromatoplate 50 nmoles of the amino acid to be measured were separately run as a reference for videodensitometric evaluation. By comparing the density of the reference amino acid to that of the individual samples the molar amount of amino acids formed or consumed in the reaction could be calculated. The present findings suggest that the chromatographic-videodensitometric microassay (CV-technique) is suitable for measuring the activity of amino acid transforming enzymes in minute amounts of tissue extracts.
Effect of Exposure to Microwaves on the Neuroendocrine Status of the Rat
1980-08-31
stem were determined by the radioenzymatic assay described by Coyle and Henry (1973) and as modified by Cuello , et al. (1973). In this assay, an extract...67, 1973. Cuello AC, Hiley R and Iverson LL: Use of catechol-o-methyltransferase for the enzyme radiochemical assay of dopamine. J. Neurochem. 21:1337
NONE
1999-07-01
Biochemistry, genetic and molecular biology aspects of either animals (including man), plants and microorganisms are studied. Topics such as cell membrane structures (including receptors), enzymatic assays, biological pathways, structural chemical analysis, metabolism, biological functions are focused. The use of radiolabelled compounds (radioassay, radioenzymatic assay, radioreceptor assay) and nuclear magnetic resonance are the most applied techniques.
NONE
2000-07-01
Several aspects concerning biochemistry and molecular biology of either animals (including man), plants and microorganisms are studied. Topics such as cell membrane structures (including receptors), enzymatic assays, biological pathways, structural chemical analysis, metabolism, biological functions are focused. The use of radiolabelled compounds (radioassay, radioenzymatic assay, radioreceptor assay) and nuclear magnetic resonance are the most applied techniques.
A simple construction for a cylindrical cloak via inverse homogenization
Anderson, Tom H; Lakhtakia, Akhlesh
2011-01-01
An effective cylindrical cloak may be conceptualized as an assembly of adjacent local neighbourhoods, each of which is made from a homogenized composite material (HCM). The HCM is required to be a certain uniaxial dielectric-magnetic material, characterized by positive-definite constitutive dyadics. It can arise from the homogenization of remarkably simple component materials, such as two isotropic dielectric-magnetic materials, randomly distributed as oriented spheroidal particles. By carefully controlling the spheroidal shape of the component particles, a high degree of HCM anisotropy may be achieved, which is necessary for the cloaking effect to be realized. The inverse Bruggeman formalism can provide estimates of the shape and constitutive parameters for the component materials, as well as their volume fractions.
Physical librations and possible homogeneity of natural moons from astrometry
Lainey, Valery; Cooper, Nicholas; Murray, Carl; Noyelles, Benoît; Pasewladt, Andreas; Robert, Vincent; Rosenblatt, Pascal; Thuillot, William
2016-10-01
Astrometry is the discipline that aims to provide positions of celestial objects in space with the highest accuracy. Thanks to recent space missions like Mars Express and Cassini, astrometric measurements of moons have allowed the probing of the gravity environment of their systems with unprecedented resolution. Here we focus on the possible determination of physical librations on the rotation of the moons, by modelling their effects on the moons' orbits. Assuming a homogeneous density, a theoretical expectation of the main libration can be computed and compared with possible observed values obtained indirectly from the orbit. In this work, we obtain for Phobos a physical libration of 1.04 +/- 0.02 degrees, in agreement with a homogeneous interior. The case of some of the inner moons of Saturn will be addressed, also.
Some variance reduction methods for numerical stochastic homogenization.
Blanc, X; Le Bris, C; Legoll, F
2016-04-28
We give an overview of a series of recent studies devoted to variance reduction techniques for numerical stochastic homogenization. Numerical homogenization requires that a set of problems is solved at the microscale, the so-called corrector problems. In a random environment, these problems are stochastic and therefore need to be repeatedly solved, for several configurations of the medium considered. An empirical average over all configurations is then performed using the Monte Carlo approach, so as to approximate the effective coefficients necessary to determine the macroscopic behaviour. Variance severely affects the accuracy and the cost of such computations. Variance reduction approaches, borrowed from other contexts in the engineering sciences, can be useful. Some of these variance reduction techniques are presented, studied and tested here.
The IDSA and the homogeneous sphere: Issues and possible improvements
Michaud, Jérôme
2016-01-01
In this paper, we are concerned with the study of the Isotropic Diffusion Source Approximation (IDSA) (Baxter et al., Phys. Rev. E 73, 046118, 2006) of radiative transfer. After having recalled well-known limits of the radiative transfer equation, we present the IDSA and adapt it to the case of the homogeneous sphere. We then show that for this example the IDSA suffers from severe numerical difficulties. We argue that these difficulties originate in the min-max switch coupling mechanism used in the IDSA. To overcome this problem we reformulate the IDSA to avoid the problematic coupling. This allows us to access the modeling error of the IDSA for the homogeneous sphere test case. The IDSA is shown to overestimate the streaming component, hence we propose a new version of the IDSA which is numerically shown to be more accurate than the old one. Analytical results and numerical tests are provided to support the accuracy of the new proposed approximation.
Homogeneous Catalysis with Metal Complexes Fundamentals and Applications
Duca, Gheorghe
2012-01-01
The book about homogeneous catalysis with metal complexes deals with the description of the reductive-oxidative, metal complexes in a liquid phase (in polar solvents, mainly in water, and less in nonpolar solvents). The exceptional importance of the redox processes in chemical systems, in the reactions occuring in living organisms, the environmental processes, atmosphere, water, soil, and in industrial technologies (especially in food-processing industries) is discussed. The detailed practical aspects of the established regularities are explained for solving the specific practical tasks in various fields of industrial chemistry, biochemistry, medicine, analytical chemistry and ecological chemistry. The main scope of the book is the survey and systematization of the latest advances in homogeneous catalysis with metal complexes. It gives an overview of the research results and practical experience accumulated by the author during the last decade.
Electrical model of dielectric barrier discharge homogenous and filamentary modes
López-Fernandez, J. A.; Peña-Eguiluz, R.; López-Callejas, R.; Mercado-Cabrera, A.; Valencia-Alvarado, R.; Muñoz-Castro, A.; Rodríguez-Méndez, B. G.
2017-01-01
This work proposes an electrical model that combines homogeneous and filamentary modes of an atmospheric pressure dielectric barrier discharge cell. A voltage controlled electric current source has been utilized to implement the power law equation that represents the homogeneous discharge mode, which starts when the gas breakdown voltage is reached. The filamentary mode implies the emergence of electric current conducting channels (microdischarges), to add this phenomenon an RC circuit commutated by an ideal switch has been proposed. The switch activation occurs at a higher voltage level than the gas breakdown voltage because it is necessary to impose a huge electric field that contributes to the appearance of streamers. The model allows the estimation of several electric parameters inside the reactor that cannot be measured. Also, it is possible to appreciate the modes of the DBD depending on the applied voltage magnitude. Finally, it has been recognized a good agreement between simulation outcomes and experimental results.
Homogenization of some evolution problems in domains with small holes
Bituin Cabarrubias
2016-07-01
Full Text Available This article concerns the asymptotic behavior of the wave and heat equations in periodically perforated domains with small holes and Dirichlet conditions on the boundary of the holes. In the first part we extend to time-dependent functions the periodic unfolding method for domains with small holes introduced in [6]. Therein, the method was applied to the study of elliptic problems with oscillating coefficients in domains with small holes, recovering the homogenization result with a "strange term" originally obtained in [11] for the Laplacian. In the second part we obtain some homogenization results for the wave and heat equations with oscillating coefficients in domains with small holes. The results concerning the wave equation extend those obtained in [12] for the case where the elliptic part of the operator is the Laplacian.
Emergence of Space-Time from Topologically Homogeneous Causal Networks
D'Ariano, Giacomo Mauro
2011-01-01
In this paper we study the emergence of Minkowski space-time from a causal network. Differently from previous approaches, we require the network to be topologically homogeneous, so that the metric is derived from pure event-counting. Emergence from events has an operational motivation in requiring that every physical quantity---including space-time---be defined through precise measurement procedures. Topological homogeneity is a requirement for having space-time metric emergent from the pure topology of causal connections, whereas physically corresponds to the universality of the physical law. We analyze in detail the case of 1+1 dimension. Coordinate systems are established via an Einsteinian protocol, and lead to a digital version of the Lorentz transformations. In a computational analogy, the foliation construction can also be regarded as the synchronization with a global clock of the calls to independent subroutines (corresponding to the causally independent events) in a parallel distributed computation, ...
Homogenization of long fiber reinforced composites including fiber bending effects
Poulios, Konstantinos; Niordson, Christian F.
2016-09-01
This paper presents a homogenization method, which accounts for intrinsic size effects related to the fiber diameter in long fiber reinforced composite materials with two independent constitutive models for the matrix and fiber materials. A new choice of internal kinematic variables allows to maintain the kinematics of the two material phases independent from the assumed constitutive models, so that stress-deformation relationships, can be expressed in the framework of hyper-elasticity and hyper-elastoplasticity for the fiber and the matrix materials respectively. The bending stiffness of the reinforcing fibers is captured by higher order strain terms, resulting in an accurate representation of the micro-mechanical behavior of the composite. Numerical examples show that the accuracy of the proposed model is very close to a non-homogenized finite-element model with an explicit discretization of the matrix and the fibers.
Exploring the Kibble-Zurek mechanism with homogeneous Bose gases
Beugnon, J
2016-01-01
Out-of-equilibrium phenomena is a subject of considerable interest in many fields of physics. Ultracold quantum gases, which are extremely clean, well-isolated and highly controllable systems, offer ideal platforms to investigate this topic. The recent progress in tailoring trapping potentials now allows the experimental production of homogeneous samples in custom geometries, which is a key advance for studies of the emergence of coherence in interacting quantum systems. Here we review recent experiments in which temperature quenches have been performed across the Bose-Einstein condensation (BEC) phase transition in an annular geometry and in homogeneous 3D and quasi-2D gases. Combined, these experiments give a comprehensive picture of the Kibble-Zurek (KZ) scenario through complementary measurements of correlation functions and topological defects density. They also allow the measurement of KZ scaling laws, the direct confirmation of the "freeze-out" hypothesis that underlies the KZ theory, and the extractio...
Self-formed waterfall plunge pools in homogeneous rock
Scheingross, Joel S.; Lo, Daniel Y.; Lamb, Michael P.
2017-01-01
Waterfalls are ubiquitous, and their upstream propagation can set the pace of landscape evolution, yet no experimental studies have examined waterfall plunge pool erosion in homogeneous rock. We performed laboratory experiments, using synthetic foam as a bedrock simulant, to produce self-formed waterfall plunge pools via particle impact abrasion. Plunge pool vertical incision exceeded lateral erosion by approximately tenfold until pools deepened to the point that the supplied sediment could not be evacuated and deposition armored the pool bedrock floor. Lateral erosion of plunge pool sidewalls continued after sediment deposition, but primarily at the downstream pool wall, which might lead to undermining of the plunge pool lip, sediment evacuation, and continued vertical pool floor incision in natural streams. Undercutting of the upstream pool wall was absent, and our results suggest that vertical drilling of successive plunge pools is a more efficient waterfall retreat mechanism than the classic model of headwall undercutting and collapse in homogeneous rock.
Effectiveness of layered stemming in comparison with homogeneous stemming
Pitts, J. H.
1976-04-01
Stemming materials utilized to fill emplacement holes at the Nevada Test Site must have a permeability small enough to prevent release of radioactive materials into the atmosphere following an experimental underground nuclear explosion. Ideal gas flow characteristic of a layered stemming column in comparison to a homogeneous stemming column when one end is exposed to a noncondensible gas source at constant pressure was examined. Although the analysis was completed in one-dimensional Cartesian coordinates, extension of the results is two-dimensional flow fields is approximated by considering parallel flow paths around the low permeability layers when the formation permeability is large. Application to cases where condensible gases are present, can be expected to give similar comparative results between layered and homogeneous stemming columns. (TFD)
Representations of Homogeneous Quantum Lévy Fields
V P Belavkin; L Gregory
2006-11-01
We study homogeneous quantum Lévy processes and fields with independent additive increments over a noncommutative ∗-monoid. These are described by infinitely divisible generating state functionals, invariant with respect to an endomorphic injective action of a symmetry semigroup. A strongly covariant GNS representation for the conditionally positive logarithmic functionals of these states is constructed in the complex Minkowski space in terms of canonical quadruples and isometric representations on the underlying pre-Hilbert field space. This is of much use in constructing quantum stochastic representations of homogeneous quantum Lévy fields on Itô monoids, which is a natural algebraic way of defining dimension free, covariant quantum stochastic integration over a space-time indexing set.
Effect of lead attenuators on dose in homogeneous phantoms
El-Khatib, E.E.; Podgorsak, E.B.; Pla, C.
1986-11-01
In radiotherapy, the radiation beam is sometimes shaped so as to deliver different doses to different organs or give a homogeneous dose to structures of different densities. This objective is achieved by the use of attenuating materials introduced into the beam. These attenuators alter the primary as well as the scattered radiation components of the beam. There is at present no accurate method of dose calculation for these situations. Most calculations are performed considering only the effect of the attenuators on the primary radiation beam and can produce large errors in dosimetry. In the present study, the broad beam attenuation is investigated in homogeneous phantoms for various radiation field sizes, photon beam energies, and depths in phantom. A calculational method taking account of primary as well as first scatter radiation is developed. This method predicts reasonably well the transmission through lead attenuators for the various experimental conditions investigated.
Role of Clustering Coefficient on Cooperation Dynamics in Homogeneous Networks
WU Gang; GAO Kun; YANG Han-Xin; WANG Bing-Hong
2008-01-01
Based on previous works, we give further investigations on the Prisoners' Dilemma Game (PDG) on two different types of homogeneous networks, i.e. the homogeneous small-world network (HSWN) and the regular ring graph. We find that the so-called resonance-like character can occur on both the networks. Different from the viewpoint in previous publications, we think the small-world effect may be unnecessary to produce this character. Therefore, over these two types of networks, we suggest a common understanding in the viewpoint of clustering coefficient. Detailed simulation results can sustain our viewpoint quite well. Furthermore, we investigate the Snowdrift Game (SG) on the same networks. The difference between the outputs of the PDG and the SG can also sustain our viewpoint.
Lifting, restricting and sifting integral points on affine homogeneous varieties
Gorodnik, Alexander
2010-01-01
In a previous paper {GN2} an effective solution of the lattice point counting problem in general domains in semisimple S-algebraic groups and affine symmetric varieties was established. The method relies on the mean ergodic theorem for the action of G on G/Gamma, and implies uniformity in counting over families of lattice subgroups admitting a uniform spectral gap. In the present paper we extend some methods developed in {NS} and use them to establish several useful consequences of this property, including : Effective upper bounds on lifting for solutions of congruences in affine homogeneous varieties, effective upper bounds on the number of integral points on general subvarieties of semisimple group varieties, effective lower bounds on the number of almost prime points on symmetric varieties, and effective upper bounds on almost prime solutions of Linnik-type congruence problems in homogeneous varieties.
New method to quantitatively evaluate the homogeneity of asphalt mixtures
WU Wen-liang; ZHANG Xiao-ning; WANG Duan-yi; LI Zhi
2009-01-01
To evaluate the homogeneity of asphalt mixtures, the images of sections obtained by cutting the as-phah mixtures specimen horizontally or vertically were analyzed with digital image processing techniques, and the particle area ratio was achieved by applying sector scan for horizontal specimen and vertical scan for vertical one. The research result indicates that the influence of random distribution of aggregates in cutting the specimen can be eliminated by using colored aggregates to distinguish coarse and fine aggregates and using color threshold to segment the images. Choosing three typical gradations, proving particle area ratio obeying normal distribution and using the variability of particle area ratio as an index, it is feasible to quantitatively evaluate the homogenei-ty of asphalt mixtures.
Waveform relaxation for the computational homogenization of multiscale magnetoquasistatic problems
Niyonzima, I.; Geuzaine, C.; Schöps, S.
2016-12-01
This paper proposes the application of the waveform relaxation method to the homogenization of multiscale magnetoquasistatic problems. In the monolithic heterogeneous multiscale method, the nonlinear macroscale problem is solved using the Newton-Raphson scheme. The resolution of many mesoscale problems per Gauß point allows to compute the homogenized constitutive law and its derivative by finite differences. In the proposed approach, the macroscale problem and the mesoscale problems are weakly coupled and solved separately using the finite element method on time intervals for several waveform relaxation iterations. The exchange of information between both problems is still carried out using the heterogeneous multiscale method. However, the partial derivatives can now be evaluated exactly by solving only one mesoscale problem per Gauß point.
Examination of homogeneity of selected Irish pooling groups
S. Das
2011-03-01
Full Text Available Flood frequency analysis is a necessary and important part of flood risk assessment and management studies. Regional flood frequency methods, in which flood data from groups of catchments are pooled together in order to enhance the precision of flood estimates at project locations, is an accepted part of such studies. This enhancement of precision is based on the assumption that catchments so pooled together are homogeneous in their flood producing properties. If homogeneity is assured then a homogeneous pooling group of sites lead to a reduction in the error of quantile estimates, relative to estimators based on single at-site data series alone. Homogeneous pooling groups are selected by using a previously nominated rule and this paper examines how effective one such rule is in selecting homogeneous groups. In this paper a study, based on annual maximum series obtained from 85 Irish gauging stations, examines how successful a common method of identifying pooling group membership is in selecting groups that actually are homogeneous. Each station has its own unique pooling group selected by use of a Euclidean distance measure in catchment descriptor space, commonly denoted d_{ij} and with a minimum of 500 station years of data in the pooling group. It was found that d_{ij} could be effectively defined in terms of catchment area, mean rainfall and baseflow index. The study then investigated how effective this selected method is in selecting groups of catchments that are actually homogenous as indicated by their L-Cv values. The sampling distribution of L-CV (t_{2} in each pooling group and the 95% confidence limits about the pooled estimate of t_{2} are obtained by simulation. The t_{2} values of the selected group members are compared with these confidence limits both graphically and numerically. Of the 85 stations, only 1 station's pooling group members have
Mixing and chemical reaction in sheared and nonsheared homogeneous turbulence
Leonard, Andy D.; Hill, James C.
1992-01-01
Direct numerical simulations were made to examine the local structure of the reaction zone for a moderately fast reaction between unmixed species in decaying, homogeneous turbulence and in a homogeneous turbulent shear flow. Pseudospectral techniques were used in domains of 64 exp 3 and higher wavenumbers. A finite-rate, single step reaction between non-premixed reactants was considered, and in one case temperature-dependent Arrhenius kinetics was assumed. Locally intense reaction rates that tend to persist throughout the simulations occur in locations where the reactant concentration gradients are large and are amplified by the local rate of strain. The reaction zones are more organized in the case of a uniform mean shear than in isotropic turbulence, and regions of intense reaction rate appear to be associated with vortex structures such as horseshoe vortices and fingers seen in mixing layers. Concentration gradients tend to align with the direction of the most compressive principal strain rate, more so in the isotropic case.
Homogenization of diffusion processes on scale-free metric networks
Fernando A. Morales
2016-10-01
Full Text Available This work studies the homogenization of diffusion processes on scale-free metric graphs, using weak variational formulations. The oscillations of the diffusion coefficient along the edges of a metric graph induce internal singularities in the global system which, together with the high complexity of large networks constitute significant difficulties in the direct analysis of the problem. At the same time, these facts also suggest homogenization as a viable approach for modeling the global behavior of the problem. To that end, we study the asymptotic behavior of a sequence of boundary problems defined on a nested collection of metric graphs. This paper presents the weak variational formulation of the problems, the convergence analysis of the solutions and some numerical experiments.
Homogeneous Earthquake Faulting, Stress and Fault Strength on Kilometer Scales
Hardebeck, J. L.
2006-12-01
I investigate small-scale fault structure using three new high-quality focal mechanism datasets of small (MLoma Prieta earthquake. I quantify the degree of mechanism variability on a range of length scales, by comparing the hypocentral distance between every pair of events and the angular difference between their focal mechanisms. I explore the implications of focal mechanism variability for the heterogeneity or homogeneity of stress and fault strength on various length scales. Focal mechanisms are very similar, often identical to within the 1σ uncertainty of ~25°, on small length scales of effect of uncertainty in earthquake locations and focal mechanisms on the apparent mechanism variability. The result that fault geometry, stress and fault strength are generally homogeneous on ~10 km length scales is encouraging for understanding earthquake physics. It may be possible to measure these parameters with enough precision to be useful in studying and modeling large earthquakes and the behavior of major faults.
Semi-classical States in Homogeneous Loop Quantum Cosmology
Tan, H; Ma, Yongge; Tan, Huahai
2006-01-01
Semi-classical states in homogeneous loop quantum cosmology (LQC) are constructed by two different ways. In the first approach, we firstly construct an exponentiated annihilation operator. Then a kind of semi-classical (coherent) state is obtained by solving the eigen-equation of that operator. Moreover, we use these coherent states to analyze the semi-classical limit of the quantum dynamics. It turns out that the Hamiltonian constraint operator employed currently in homogeneous LQC has correct classical limit with respect to the coherent states. In the second approach, the other kind of semi-classical state is derived from the mathematical construction of coherent states for compact Lie groups due to Hall.
Microfluidic converging/diverging channels optimised for homogeneous extensional deformation
Zografos, K.; Oliveira, M. S. N.
2016-01-01
In this work, we optimise microfluidic converging/diverging geometries in order to produce constant strain-rates along the centreline of the flow, for performing studies under homogeneous extension. The design is examined for both two-dimensional and three-dimensional flows where the effects of aspect ratio and dimensionless contraction length are investigated. Initially, pressure driven flows of Newtonian fluids under creeping flow conditions are considered, which is a reasonable approximation in microfluidics, and the limits of the applicability of the design in terms of Reynolds numbers are investigated. The optimised geometry is then used for studying the flow of viscoelastic fluids and the practical limitations in terms of Weissenberg number are reported. Furthermore, the optimisation strategy is also applied for electro-osmotic driven flows, where the development of a plug-like velocity profile allows for a wider region of homogeneous extensional deformation in the flow field. PMID:27478523
Hydrogen Production by Homogeneous Catalysis: Alcohol Acceptorless Dehydrogenation
Nielsen, Martin
2015-01-01
in hydrogen production from biomass using homogeneous catalysis. Homogeneous catalysis has the advance of generally performing transformations at much milder conditions than traditional heterogeneous catalysis, and hence it constitutes a promising tool for future applications for a sustainable energy sector......, and are fundamental for the thrive of almost all business fields. The latter include the industries of agriculture, food additives, pharmaceuticals, electronics, plastic, fragrances, and more. Today, the major source of both energy and bulk chemicals is fossil fuels, being responsible for more than 80 % of the energy...... dehydrogenation. The third chapter, Biorelevant Substrates, concentrates on the use of alcohols such as ethanol, which are biomass related. The topic is alcohol acceptorless dehydrogenation reactions for both H2 production and the concurrent synthetic application. Finally, Chap. 4, Substrates for H2 Storage...
Can photonic crystals be homogenized in higher bands?
Markel, Vadim A
2015-01-01
We consider the conditions under which photonic crystals (PCs) can be viewed as electromagnetically homogeneous at frequencies in the higher photonic bands and, in particular, near the higher-order $\\Gamma$-points. We show that the observation that a purely real isofrequency line of the PC is close to a mathematical circle is insufficient for establishing homogenizability. Complex dispersion points must be included into consideration even in the case of strictly non-absorbing materials. By applying a more careful analysis to the dispersion relations and complex isofrequency lines, we have found that two-dimensional PCs with $C_4$ and $C_6$ symmetries are not electromagnetically homogeneous in the higher photonic bands in spite of the fact that, at some particular frequencies, the real isofrequency lines of these PCs can be circular with high precision.
The isotropic blackbody CMB as evidence for a homogeneous universe
Clifton, Timothy; Bull, Philip
2011-01-01
The question of whether the Universe is spatially homogeneous and isotropic on the largest scales is of fundamental importance to cosmology, but has not yet been answered decisively. Surprisingly, neither an isotropic primary CMB nor combined observations of luminosity distances and galaxy number counts are sufficient to establish such a result. The inclusion of the Sunyaev-Zel'dovich effect in CMB observations, however, dramatically improves this situation. We show that even a solitary observer who sees an isotropic blackbody CMB can conclude that the universe is homogeneous and isotropic in their causal past when the Sunyaev-Zel'dovich effect is present. Critically, however, the CMB must either be viewed for an extended period of time, or CMB photons that have scattered more than once must be detected. This result provides a theoretical underpinning for testing the Cosmological Principle with observations of the CMB alone.
Chromosome aberration assays in Allium
Grant, W.F.
1982-01-01
The common onion (Allium cepa) is an excellent plant for the assay of chromosome aberrations after chemical treatment. Other species of Allium (A. cepa var. proliferum, A. carinatum, A. fistulosum and A. sativum) have also been used but to a much lesser extent. Protocols have been given for using root tips from either bulbs or seeds of Allium cepa to study the cytological end-points, such as chromosome breaks and exchanges, which follow the testing of chemicals in somatic cells. It is considered that both mitotic and meiotic end-points should be used to a greater extent in assaying the cytogenetic effects of a chemical. From a literature survey, 148 chemicals are tabulated that have been assayed in 164 Allium tests for their clastogenic effect. Of the 164 assays which have been carried out, 75 are reported as giving a positive reaction, 49 positive and with a dose response, 1 positive and temperature-related, 9 borderline positive, and 30 negative; 76% of the chemicals gave a definite positive response. It is proposed that the Allium test be included among those tests routinely used for assessing chromosomal damage induced by chemicals.
Electromagnetic Radiation in a Uniformly Moving, Homogeneous Medium
Johannsen, Günther
1972-01-01
A new method of treating radiation problems in a uniformly moving, homogeneous medium is presented. A certain transformation technique in connection with the four-dimensional Green's function method makes it possible to elaborate the Green's functions of the governing differential equations in th...... in the rest system of the medium, whereas the final integrals determining the field may be calculated in the rest system of the source. ©1972 The American Institute of Physics...
Homogeneity Property of Besov and Triebel-Lizorkin Spaces
Cornelia Schneider
2012-01-01
Full Text Available We consider the classical Besov and Triebel-Lizorkin spaces defined via differences and prove a homogeneity property for functions with bounded support in the frame of these spaces. As the proof is based on compact embeddings between the studied function spaces, we present also some results on the entropy numbers of these embeddings. Moreover, we derive some applications in terms of pointwise multipliers.
On the transition between heterogeneous and homogeneous freezing
K. Gierens
2003-01-01
Full Text Available Box model simulations of an uplifting and adiabatically cooling cloud of aerosol have been performed in order to study the transition between cirrus formation dominated by homogeneous nucleation of ice to that dominated by heterogeneous nucleation. The aerosol was assumed to consist of an internal mixture of sulfuric acid solution droplets with inclusions of soot. The parametrisation of De Mott et al. (1997 was used to simulate the heterogeneous nucleation of ice in such droplets with soot inclusions. The simulations show that the transition from heterogeneous to homogeneous nucleation occurs over a narrow range of soot concentration. Thus it seems to be possible to fix critical concentrations of heterogeneous ice nuclei which must be exceeded if heterogeneous freezing dominates cirrus formation. A formula has been derived that allows to compute the critical concentrations of heterogeneous ice nuclei as a function of temperature, updraft speed, ambient pressure, and supersaturation at which heterogeneous freezing occurs. Generally, homogeneous nucleation dominates in regions with updrafts stronger than 20 cm s -1, with the exception of heavily polluted areas which could be common in the northern hemisphere due to air traffic, where updrafts of the order 1 m s-1 may be necessary to render heterogeneous nucleation unimportant. According to the present results it cannot be excluded that heterogeneous nucleation plays a more important role for cirrus formation in the northern midlatitudes than anywhere else. A possible consequence of these results is that air pollution may lead to a higher coverage of cirrus clouds, but then these clouds will be optically thinner than clouds formed by homogeneous freezing, with the exception of regions where condensation trails are frequent.
Green's function of homogeneous overmoded waveguide with finite conductivity walls
Saldin, E L; Yurkov, M V
2000-01-01
We describe an approach for developing the numerical simulation codes for the FEL amplifier with the homogeneous overmode waveguide. The radiation field are calculated using Green's function method. We start with the rigorous solutions for the eigenfunctions of a passive waveguide. Using these eigenfunctions, we find the Green's function. Finally, the Green's function is simplified using paraxial approximation. This algorithm of electromagnetic field calculation can be implemented in linear and nonlinear code for simulation of the waveguide FEL.
Quantum product and parabolic orbits in homogeneous spaces
Pech, Clélia
2012-01-01
Chaput, Manivel and Perrin proved a formula describing the quantum product by Schubert classes associated to cominuscule weights in a rational projective homogeneous space X. In the case where X has Picard rank one, we link this formula to the stratification of X by P-orbits, where P is the parabolic subgroup associated to the cominuscule weight. We deduce a decomposition of the Hasse diagram of X, i.e the diagram describing the cup-product with the hyperplane class.
Uniqueness of the differential Mueller matrix of uniform homogeneous media.
Devlaminck, Vincent; Ossikovski, Razvigor
2014-06-01
We show that the differential matrix of a uniform homogeneous medium containing birefringence may not be uniquely determined from its Mueller matrix, resulting in the potential existence of an infinite set of elementary polarization properties parameterized by an integer parameter. The uniqueness depends on the symmetry properties of a special differential matrix derived from the eigenvalue decomposition of the Mueller matrix. The conditions for the uniqueness of the differential matrix are identified, physically discussed, and illustrated in examples from the literature.
Comment on evidence for surface-initiated homogenous nucleation
J. E. Kay
2003-01-01
Full Text Available We investigate theoretical, laboratory, and atmospheric evidence for a recently proposed hypothesis: homogenous ice nucleation occurs at the surface, not in the volume, of supercooled water drops. Using existing thermodynamic arguments, laboratory experiments, and atmospheric data, we conclude that ice embryo formation at the surface cannot be confirmed or disregarded. Ice nucleation rates measured as a function of drop size in an air ambient could help distinguish between volume and surface nucleation rates.
Upper Maxwellian bounds for the spatially homogeneous Boltzmann equation
Gamba, I. M.; Panferov, V.; Villani, C.
2007-01-01
For the spatially homogeneous Boltzmann equation with cutoff hard potentials it is shown that solutions remain bounded from above, uniformly in time, by a Maxwellian distribution, provided the initial data have a Maxwellian upper bound. The main technique is based on a comparison principle that uses a certain dissipative property of the linear Boltzmann equation. Implications of the technique to propagation of upper Maxwellian bounds in the spatially-inhomogeneous case are discussed.
On generalized Gaussian free fields and stochastic homogenization
Gu, Yu; Mourrat, Jean-Christophe
2016-01-01
We study a generalization of the notion of Gaussian free field (GFF). Although the extension seems minor, we first show that a generalized GFF does not satisfy the spatial Markov property, unless it is a classical GFF. In stochastic homogenization, the scaling limit of the corrector is a possibly generalized GFF described in terms of an "effective fluctuation tensor" that we denote by $\\mathsf{Q}$. We prove an expansion of $\\mathsf{Q}$ in the regime of small ellipticity ratio. This expansion ...
A Simple First-Principles Homogenization Theory for Chiral Metamaterials
Carlo Rizza
2015-04-01
Full Text Available We discuss a simple first-principles homogenization theory for describing, in the long-wavelength limit, the effective bianisotropic response of a periodic metamaterial composite without intrinsic chiral and magnetic inclusions. In the case where the dielectric contrast is low, we obtain a full analytical description which can be considered the extension of Landau-Lifshitz-Looyenga effective-medium formulation in the context of periodic metamaterials.
Optimal Layout of Transshipment Facilities on An Infinite Homogeneous Plane
Xie, Weijun; Ouyang, Yanfeng
2014-01-01
This paper studies optimal spatial layout of transshipment facilities and the corresponding service regions on an infinite homogeneous plane $\\mathbb{R}^2$ that minimize the total cost for facility set-up, outbound delivery and inbound replenishment transportation. The problem has strong implications in the context of freight logistics and transit system design. This paper first focuses on a Euclidean plane and presents a new proof for the known Gersho's conjecture, which states that the opti...
Simulator for SUPO, a Benchmark Aqueous Homogeneous Reactor (AHR)
Klein, Steven Karl [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Determan, John C. [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
2015-10-14
A simulator has been developed for SUPO (Super Power) an aqueous homogeneous reactor (AHR) that operated at Los Alamos National Laboratory (LANL) from 1951 to 1974. During that period SUPO accumulated approximately 600,000 kWh of operation. It is considered the benchmark for steady-state operation of an AHR. The SUPO simulator was developed using the process that resulted in a simulator for an accelerator-driven subcritical system, which has been previously reported.
Research on homogeneous deformation of electromagnetic incremental tube bulging
Cui, Xiaohui; Mo, Jianhua; Li, Jianjun
2014-01-01
The electromagnetic incremental forming (EMIF) method is used for tube forming process. Suitable 2D FE models are designed to predict the forming process with a moving coil. In comparison with experimental values, simulation method can obtain accurate results. Then, effect factors named overlapping ration of adjacent discharge positions, discharge voltage, forming sequence and die dimension on tube homogeneous deformation are discussed. The result demonstrates that it is feasib...
POLYMERIZATION MECHANISM OF DIENES WITH HOMOGENEOUS RARE EARTH CATALYSTS
JIN Yingtai; ZHANG Xitian; PEI Fengkui; WU Yue
1990-01-01
The reaction mechanisms ofdiene polymerization with homogeneous rare earth catalyst are studied by means of the spectra of 1H- NM R, one- and two- dimensions 13C-NMR. Based on the data of above NMR spectra, it is proposed that the polymerization reaction proceeds according to the following mechanism: η4-diene (cis- (→)trans- )and η3-allyl (syn- (→)anti- ).
Studies of the Beetle 1.2 Pipeline Homogeneity
Agari, M; Blouw, J; Schmelling, M; Hofmann, W; Schwingenheuer, B; Pugatch, V; Volyanskyy, D; Jiménez-Otero, S; Tran, M T; Voss, H; Bernhard, R P; Köstner, S; Lehner, F; Lois, C; Needham, M; Steinkamp, O; Straumann, U; Vollhardt, A
2003-01-01
The pipeline homogeneity in general and the behaviour of the edge channels of the Beetle 1.2 readout chip [1] were studied with data taken during the Silicon Tracker test beam period in May 2003. A contribution of roughly 10\\% from pipeline inhomogeneities to the strip noise was observed. All channels including the first and the last one were found to be fully functional.
Homogenization of soil properties map by Principal Component Analysis
Valverde Arias, Omar; Garrido, Alberto; Villeta, Maria; Tarquis, Ana Maria
2016-04-01
It is widely known that extreme climatic phenomena occur with more intensity and frequency. This fact has put more pressure over farming, becoming very important to implement agriculture risk management policies by governments and institutions. One of the main strategies is transfer risk by agriculture insurance. Agriculture insurance based in indexes has gained importance in the last decade. And consist in a comparison between measured index values with a defined threshold that triggers damage losses. However, based index insurance could not be based on an isolated measurement. It is necessary to be integrated in a complete monitoring system that uses many sources of information and tools. For example, index influence areas, crop production risk maps, crop yields, claim statistics, and so on. To establish index influence area is necessary to have a secondary information that show us homogeneous climatic and soil areas, which inside of each homogeneous classes, index measurements on crops of interest are going to be similar, and in this way reduce basis risk. But it is necessary an efficient method to accomplish this aim, to get homogeneous areas that not depends on only in expert criteria and that could be widely used, for this reason this study asses two conventional agricultural and geographic methods (control and climatic maps) based in expert criteria, and one classical statistical method of multi-factorial analysis (factorial map), all of them to homogenize soil and climatic characteristics. Resulting maps were validated by agricultural and spatial analysis, obtaining very good results in statistical method (Factorial map) that proves to be an efficient and accuracy method that could be used for similar porpoises.
Generating and controlling homogeneous air turbulence using random jet arrays
Carter, Douglas; Petersen, Alec; Amili, Omid; Coletti, Filippo
2016-12-01
The use of random jet arrays, already employed in water tank facilities to generate zero-mean-flow homogeneous turbulence, is extended to air as a working fluid. A novel facility is introduced that uses two facing arrays of individually controlled jets (256 in total) to force steady homogeneous turbulence with negligible mean flow, shear, and strain. Quasi-synthetic jet pumps are created by expanding pressurized air through small straight nozzles and are actuated by fast-response low-voltage solenoid valves. Velocity fields, two-point correlations, energy spectra, and second-order structure functions are obtained from 2D PIV and are used to characterize the turbulence from the integral-to-the Kolmogorov scales. Several metrics are defined to quantify how well zero-mean-flow homogeneous turbulence is approximated for a wide range of forcing and geometric parameters. With increasing jet firing time duration, both the velocity fluctuations and the integral length scales are augmented and therefore the Reynolds number is increased. We reach a Taylor-microscale Reynolds number of 470, a large-scale Reynolds number of 74,000, and an integral-to-Kolmogorov length scale ratio of 680. The volume of the present homogeneous turbulence, the largest reported to date in a zero-mean-flow facility, is much larger than the integral length scale, allowing for the natural development of the energy cascade. The turbulence is found to be anisotropic irrespective of the distance between the jet arrays. Fine grids placed in front of the jets are effective at modulating the turbulence, reducing both velocity fluctuations and integral scales. Varying the jet-to-jet spacing within each array has no effect on the integral length scale, suggesting that this is dictated by the length scale of the jets.
Radiation Resistance and Gain of Homogeneous Ring Quasi-Array
Knudsen, H. L.
1954-01-01
In a previous paper homogeneous ring quasi-arrays of tangential or radial dipoles were introduced, i.e. systems of dipoles arranged equidistantly along a circle, the dipoles being oriented in tangential or radial directions and carrying currents with the same amplitude, but with a phase......-arrays of an infinite number of tangential or radial dipoles, and from the results arrived at conclusions are drawn as to the applicability of such antenna systems...
Eigenview on Jones matrix models of homogeneous anisotropic media
Savenkov S.
2010-06-01
Full Text Available The polarization of light when it passes through optical medium can change as a result of change in the amplitude (dichroism or phase shift (birefringence of the electric vector. The anisotropic properties of media can be determined from these two optical effects. Our main concern here is to revisit the factor of eigenpolarizations and eigenvalues in modeling of polarization properties of homogeneous media and elucidate certain new features in polarization behavior of birefringent and dichroic media.
Desertification, salinization, and biotic homogenization in a dryland river ecosystem
Miyazono, S.; Patino, Reynaldo; Taylor, C.M.
2015-01-01
This study determined long-term changes in fish assemblages, river discharge, salinity, and local precipitation, and examined hydrological drivers of biotic homogenization in a dryland river ecosystem, the Trans-Pecos region of the Rio Grande/Rio Bravo del Norte (USA/Mexico). Historical (1977-1989) and current (2010-2011) fish assemblages were analyzed by rarefaction analysis (species richness), nonmetric multidimensional scaling (composition/variability), multiresponse permutation procedures (composition), and paired t-test (variability). Trends in hydrological conditions (1970s-2010s) were examined by Kendall tau and quantile regression, and associations between streamfiow and specific conductance (salinity) by generalized linear models. Since the 1970s, species richness and variability of fish assemblages decreased in the Rio Grande below the confluence with the Rio Conchos (Mexico), a major tributary, but not above it. There was increased representation of lower-flow/higher-salinity tolerant species, thus making fish communities below the confluence taxonomically and functionally more homogeneous to those above it. Unlike findings elsewhere, this biotic homogenization was due primarily to changes in the relative abundances of native species. While Rio Conchos discharge was > 2-fold higher than Rio Grande discharge above their confluence, Rio Conchos discharge decreased during the study period causing Rio Grande discharge below the confluence to also decrease. Rio Conchos salinity is lower than Rio Grande salinity above their confluence and, as Rio Conchos discharge decreased, it caused Rio Grande salinity below the confluence to increase (reduced dilution). Trends in discharge did not correspond to trends in precipitation except at extreme-high (90th quantile) levels. In conclusion, decreasing discharge from the Rio Conchos has led to decreasing flow and increasing salinity in the Rio Grande below the confluence. This spatially uneven desertification and
Homogeneous Charge Compression Ignition Combustion of Dimethyl Ether
Pedersen, Troels Dyhr; Schramm, Jesper
2011-01-01
This thesis is based on experimental and numerical studies on the use of dimethyl ether (DME) in the homogeneous charge compression ignition (HCCI) combustion process. The first paper in this thesis was published in 2007 and describes HCCI combustion of pure DME in a small diesel engine. The tests were designed to investigate the effect of engine speed, compression ratio and equivalence ratio on the combustion timing and the engine performance. It was found that the required compression ratio...
Genetic background of clinical homogeneity of phenylketonuria in Poland.
Jaruzelska, J; Matuszak, R; Lyonnet, S; Rey, F; Rey, J; Filipowicz, J; Borski, K; Munnich, A.
1993-01-01
In order to elucidate the clinical homogeneity and severity of the hyperphenylalaninaemias in Poland, a total of 71 children with typical phenylketonuria (PKU) originating from western and northern Poland were screened for 13 mutations in the phenylalanine hydroxylase (PAH) gene. Eighty percent of all PKU alleles tested were found to carry an identified mutation. One mutation, namely the R408W mutation, accounted for more than 63% of mutant PAH alleles in Poland, the other 27% being accounted...
The isotropic blackbody CMB as evidence for a homogeneous universe
Clifton, Timothy; Clarkson, Chris; Bull, Philip
2011-01-01
The question of whether the Universe is spatially homogeneous and isotropic on the largest scales is of fundamental importance to cosmology, but has not yet been answered decisively. Surprisingly, neither an isotropic primary CMB nor combined observations of luminosity distances and galaxy number counts are sufficient to establish such a result. The inclusion of the Sunyaev-Zel'dovich effect in CMB observations, however, dramatically improves this situation. We show that even a solitary obser...
Green's function of homogeneous overmoded waveguide with finite conductivity walls
Saldin, E. L.; Schneidmiller, E. A.; Yurkov, M. V.
2000-05-01
We describe an approach for developing the numerical simulation codes for the FEL amplifier with the homogeneous overmode waveguide. The radiation field are calculated using Green's function method. We start with the rigorous solutions for the eigenfunctions of a passive waveguide. Using these eigenfunctions, we find the Green's function. Finally, the Green's function is simplified using paraxial approximation. This algorithm of electromagnetic field calculation can be implemented in linear and nonlinear code for simulation of the waveguide FEL.
Development of a multiplexed urine assay for prostate cancer diagnosis.
Vener, Tatiana; Derecho, Carlo; Baden, Jonathan; Wang, Haiying; Rajpurohit, Yashoda; Skelton, Joanne; Mehrotra, Jyoti; Varde, Shobha; Chowdary, Dondapati; Stallings, Walt; Leibovich, Bradley; Robin, Howard; Pelzer, Alexandre; Schäfer, Georg; Auprich, Marco; Mannweiler, Sebastian; Amersdorfer, Peter; Mazumder, Abhijit
2008-05-01
Several studies have demonstrated the value of DNA methylation in urine-based assays for prostate cancer diagnosis. However, a multicenter validation with a clinical prototype has not been published. We developed a multiplexed, quantitative methylation-specific polymerase chain reaction (MSP) assay consisting of 3 methylation markers, GSTP1, RARB, and APC, and an endogenous control, ACTB, in a closed-tube, homogeneous assay format. We tested this format with urine samples collected after digital rectal examination from 234 patients with prostate-specific antigen (PSA) concentrations > or =2.5 microg/L in 2 independent patient cohorts from 9 clinical sites. In the first cohort of 121 patients, we demonstrated 55% sensitivity and 80% specificity, with area under the curve (AUC) 0.69. In the second independent cohort of 113 patients, we found a comparable sensitivity of 53% and specificity of 76% (AUC 0.65). In the first cohort, as well as in a combined cohort, the MSP assay in conjunction with total PSA, digital rectal examination status, and age improved the AUC without MSP, although the difference was not statistically significant. Importantly, the GSTP1 cycle threshold value demonstrated a good correlation (R = 0.84) with the number of cores found to contain prostate cancer or premalignant lesions on biopsy. Moreover, samples that exhibited methylation for either GSTP1 or RARB typically contained higher tumor volumes at prostatectomy than those samples that did not exhibit methylation. These data confirm and extend previously reported studies and demonstrate the performance of a clinical prototype assay that should aid urologists in identifying men who should undergo biopsy.
Fourier-based strength homogenization of porous media
Bignonnet, François; Hassen, Ghazi; Dormieux, Luc
2016-11-01
An efficient numerical method is proposed to upscale the strength properties of heterogeneous media with periodic boundary conditions. The method relies on a formal analogy between strength homogenization and non-linear elasticity homogenization. The non-linear problems are solved on a regular discretization grid using the Augmented Lagrangian version of Fast Fourier Transform based schemes initially introduced for elasticity upscaling. The method is implemented for microstructures with local strength properties governed either by a Green criterion or a Von Mises criterion, including pores or rigid inclusions. A thorough comparison with available analytical results or finite element elasto-plastic simulations is proposed to validate the method on simple microstructures. As an application, the strength of complex microstructures such as the random Boolean model of spheres is then studied, including a comparison to closed-form Gurson and Eshelby based strength estimates. The effects of the microstructure morphology and the third invariant of the macroscopic stress tensor on the homogenized strength are quantitatively discussed.
Fourier-based strength homogenization of porous media
Bignonnet, François; Hassen, Ghazi; Dormieux, Luc
2016-07-01
An efficient numerical method is proposed to upscale the strength properties of heterogeneous media with periodic boundary conditions. The method relies on a formal analogy between strength homogenization and non-linear elasticity homogenization. The non-linear problems are solved on a regular discretization grid using the Augmented Lagrangian version of Fast Fourier Transform based schemes initially introduced for elasticity upscaling. The method is implemented for microstructures with local strength properties governed either by a Green criterion or a Von Mises criterion, including pores or rigid inclusions. A thorough comparison with available analytical results or finite element elasto-plastic simulations is proposed to validate the method on simple microstructures. As an application, the strength of complex microstructures such as the random Boolean model of spheres is then studied, including a comparison to closed-form Gurson and Eshelby based strength estimates. The effects of the microstructure morphology and the third invariant of the macroscopic stress tensor on the homogenized strength are quantitatively discussed.
Mathematical Structure of Loop Quantum Cosmology: Homogeneous Models
Martin Bojowald
2013-12-01
Full Text Available The mathematical structure of homogeneous loop quantum cosmology is analyzed, starting with and taking into account the general classification of homogeneous connections not restricted to be Abelian. As a first consequence, it is seen that the usual approach of quantizing Abelian models using spaces of functions on the Bohr compactification of the real line does not capture all properties of homogeneous connections. A new, more general quantization is introduced which applies to non-Abelian models and, in the Abelian case, can be mapped by an isometric, but not unitary, algebra morphism onto common representations making use of the Bohr compactification. Physically, the Bohr compactification of spaces of Abelian connections leads to a degeneracy of edge lengths and representations of holonomies. Lifting this degeneracy, the new quantization gives rise to several dynamical properties, including lattice refinement seen as a direct consequence of state-dependent regularizations of the Hamiltonian constraint of loop quantum gravity. The representation of basic operators - holonomies and fluxes - can be derived from the full theory specialized to lattices. With the new methods of this article, loop quantum cosmology comes closer to the full theory and is in a better position to produce reliable predictions when all quantum effects of the theory are taken into account.
Homogeneous CdTe quantum dots-carbon nanotubes heterostructures
Vieira, Kayo Oliveira [Grupo de Pesquisa em Química de Materiais – (GPQM), Departamento de Ciências Naturais, Universidade Federal de São João del-Rei, Campus Dom Bosco, Praça Dom Helvécio, 74, CEP 36301-160, São João del-Rei, MG (Brazil); Bettini, Jefferson [Laboratório Nacional de Nanotecnologia, Centro Nacional de Pesquisa em Energia e Materiais, CEP 13083-970, Campinas, SP (Brazil); Ferrari, Jefferson Luis [Grupo de Pesquisa em Química de Materiais – (GPQM), Departamento de Ciências Naturais, Universidade Federal de São João del-Rei, Campus Dom Bosco, Praça Dom Helvécio, 74, CEP 36301-160, São João del-Rei, MG (Brazil); Schiavon, Marco Antonio, E-mail: schiavon@ufsj.edu.br [Grupo de Pesquisa em Química de Materiais – (GPQM), Departamento de Ciências Naturais, Universidade Federal de São João del-Rei, Campus Dom Bosco, Praça Dom Helvécio, 74, CEP 36301-160, São João del-Rei, MG (Brazil)
2015-01-15
The development of homogeneous CdTe quantum dots-carbon nanotubes heterostructures based on electrostatic interactions has been investigated. We report a simple and reproducible non-covalent functionalization route that can be accomplished at room temperature, to prepare colloidal composites consisting of CdTe nanocrystals deposited onto multi-walled carbon nanotubes (MWCNTs) functionalized with a thin layer of polyelectrolytes by layer-by-layer technique. Specifically, physical adsorption of polyelectrolytes such as poly (4-styrene sulfonate) and poly (diallyldimethylammonium chloride) was used to deagglomerate and disperse MWCNTs, onto which we deposited CdTe quantum dots coated with mercaptopropionic acid (MPA), as surface ligand, via electrostatic interactions. Confirmation of the CdTe quantum dots/carbon nanotubes heterostructures was done by transmission and scanning electron microscopies (TEM and SEM), dynamic-light scattering (DLS) together with absorption, emission, Raman and infrared spectroscopies (UV–vis, PL, Raman and FT-IR). Almost complete quenching of the PL band of the CdTe quantum dots was observed after adsorption on the MWCNTs, presumably through efficient energy transfer process from photoexcited CdTe to MWCNTs. - Highlights: • Highly homogeneous CdTe-carbon nanotubes heterostructures were prepared. • Simple and reproducible non-covalent functionalization route. • CdTe nanocrystals homogeneously deposited onto multi-walled carbon nanotubes. • Efficient energy transfer process from photoexcited CdTe to MWCNTs.
How to determine composite material properties using numerical homogenization
Andreassen, Erik; Andreasen, Casper Schousboe
2014-01-01
Numerical homogenization is an efficient way to determine effective macroscopic properties, such as the elasticity tensor, of a periodic composite material. In this paper an educational description of the method is provided based on a short, self-contained Matlab implementation. It is shown how t...... cell of the periodic material can take the shape of a square, rectangle, or parallelogram, allowing for all kinds of 2D periodicities. © 2013 Elsevier B.V. All rights reserved.......Numerical homogenization is an efficient way to determine effective macroscopic properties, such as the elasticity tensor, of a periodic composite material. In this paper an educational description of the method is provided based on a short, self-contained Matlab implementation. It is shown how...... the basic code, which computes the effective elasticity tensor of a two material composite, where one material could be void, is easily extended to include more materials. Furthermore, extensions to homogenization of conductivity, thermal expansion, and fluid permeability are described in detail. The unit...
Identification of homogeneous and heterogeneous variables in pooled cohort studies.
Cheng, Xin; Lu, Wenbin; Liu, Mengling
2015-06-01
Pooled analyses integrate data from multiple studies and achieve a larger sample size for enhanced statistical power. When heterogeneity exists in variables' effects on the outcome across studies, the simple pooling strategy fails to present a fair and complete picture of the effects of heterogeneous variables. Thus, it is important to investigate the homogeneous and heterogeneous structure of variables in pooled studies. In this article, we consider the pooled cohort studies with time-to-event outcomes and propose a penalized Cox partial likelihood approach with adaptively weighted composite penalties on variables' homogeneous and heterogeneous effects. We show that our method can characterize the variables as having heterogeneous, homogeneous, or null effects, and estimate non-zero effects. The results are readily extended to high-dimensional applications where the number of parameters is larger than the sample size. The proposed selection and estimation procedure can be implemented using the iterative shooting algorithm. We conduct extensive numerical studies to evaluate the performance of our proposed method and demonstrate it using a pooled analysis of gene expression in patients with ovarian cancer. © 2015, The International Biometric Society.
Broken Ergodicity in Two-Dimensional Homogeneous Magnetohydrodynamic Turbulence
Shebalin, John V.
2010-01-01
Two-dimensional (2-D) homogeneous magnetohydrodynamic (MHD) turbulence has many of the same qualitative features as three-dimensional (3-D) homogeneous MHD turbulence.The se features include several ideal invariants, along with the phenomenon of broken ergodicity. Broken ergodicity appears when certain modes act like random variables with mean values that are large compared to their standard deviations, indicating a coherent structure or dynamo.Recently, the origin of broken ergodicity in 3-D MHD turbulence that is manifest in the lowest wavenumbers was explained. Here, a detailed description of the origins of broken ergodicity in 2-D MHD turbulence is presented. It will be seen that broken ergodicity in ideal 2-D MHD turbulence can be manifest in the lowest wavenumbers of a finite numerical model for certain initial conditions or in the highest wavenumbers for another set of initial conditions.T he origins of broken ergodicity in ideal 2-D homogeneous MHD turbulence are found through an eigen analysis of the covariance matrices of the modal probability density functions.It will also be shown that when the lowest wavenumber magnetic field becomes quasi-stationary, the higher wavenumber modes can propagate as Alfven waves on these almost static large-scale magnetic structures
Properties of subvisible cirrus clouds formed by homogeneous freezing
B. Kärcher
2002-04-01
Full Text Available Number concentrations and mean sizes of ice crystals and derived microphysical and optical properties of subvisible cirrus clouds (SVCs formed by homogeneous freezing of supercooled aerosols are investigated as a function of temperature and updraft speed of adiabatically ascending air parcels. The properties of such clouds are insensitive to variations of the aerosol number and size distribution. Based on criteria constraining the optical extinction, sedimentation time, and existence time of SVCs, longer-lived (>10 min clouds, capable of exerting a measurable radiative or chemical impact, are generated within a narrow range of updraft speeds below 1 - 2 cm s ^{-1} at temperatures below about 215K, with concentrations of ice crystals not exceeding 0.1 cm^{-3}. The clouds do not reach an equilibrium state because the ice crystals sediment out of the formation layer typically before the supersaturation is removed. Given these results, it seems likely that a limited number (<0.1 cm^{-3} of effective heterogeneous freezing nuclei that nucleate ice below the homogeneous freezing threshold can control the formation and properties of SVCs, although homogeneous freezing nuclei are far more abundant.
Surface modification of polycarbonate in homogeneous atmospheric pressure discharge
SIra, M; Trunec, D; St' ahel, P; BursIkova, V; Navratil, Z [Department of Physical Electronics, Faculty of Science, Masaryk University, Kotlarska 2, 611 37 Brno (Czech Republic)
2008-01-07
A homogeneous atmospheric pressure dielectric barrier discharge was used for the surface modification of polycarbonate (PC). The discharge was generated between two planar metal electrodes, the top electrode was covered by glass and the bottom electrode was covered by a polymer sample. The discharge burned in pure nitrogen or in a mixture of nitrogen and hydrogen. The surface properties of both treated and untreated polymers were characterized by atomic force microscopy, surface free energy (SFE) measurements and x-ray photoelectron spectroscopy. The influence of the treatment time and power input to the discharge on the surface properties of polymers was studied. The ageing of treated samples was also investigated. The treatment of polymers in the homogeneous atmospheric pressure discharge was homogeneous and the polymer surfaces showed a smaller degree of roughness in comparison with the polymer surfaces treated in a filamentary discharge. The SFE of the treated PC obtained at optimum conditions was 53 mJ m{sup -2} and the corresponding contact angle of water was 38{sup 0}. The maximum decrease in the SFE during ageing was about 13%. The analysis of the chemical composition showed an increase in the nitrogen concentration in the surface layer, but almost a zero increase in the oxygen concentration. This result was discussed concerning the measured values of the SFE measurement.
One-step assay for the quantification of T4 DNA ligase.
Franke, Steffi; Kreisig, Thomas; Buettner, Karin; Zuchner, Thole
2015-02-01
As one of the most commonly used enzyme in molecular biology, the T4 DNA ligase presents an important tool for the manipulation of DNA. T4 DNA ligase activity measurements are based on the use of radioactivity or rather labor-intense procedures including gel-based analysis. We therefore established a homogeneous T4 DNA ligase assay utilizing a specifically designed fluorescein- and dark quencher-labeled DNA molecule. Upon ligation of both DNA molecules, a quenching occurs and the fluorescence intensity decreases with increasing ligase concentrations. The assay allows a sensitive and precise quantification (CV, 4.6-5.5 %) of T4 DNA ligase activities and showed a high specificity when tested against other ligases of related and different species. Most importantly, this T4 DNA ligase assay requires only one working and incubation step before measurement can take place at room temperature and may therefore offer an interesting alternative to existing, more laborious ligase assays.
Crouvisier-Urion, Kevin; Lagorce-Tachon, Aurélie; Lauquin, Camille; Winckler, Pascale; Tongdeesoontorn, Wirongrong; Domenek, Sandra; Debeaufort, Frédéric; Karbowiak, Thomas
2017-12-01
This work investigated the impact of two homogenization treatments, High Shear (HS) and High Pressure (HP), on the structure and antioxidant activity of chitosan-lignin bio-composite films. Laser light scattering analysis revealed that smaller lignin particles were obtained after HP processing, around 0.6μm, compared to HS treatment, between 2.5 and 5μm. Moreover, these particles were more homogeneously distributed in the chitosan film matrix after HP process, while some aggregates remained after HS treatment, as highlighted by two-photon microscopy. The surface hydrophobicity of the composite films, as measured by water contact angle, increased after the two homogenization treatments. Finally, the antioxidant activity of the composite films was determined using the DPPH· assay. No significant difference in the radical scavenging activity was noticeable, neither after HS nor HP processing. However, a migration of lignin residues from the film to the extraction medium was noticed, particularly for HP process. Copyright © 2017. Published by Elsevier Ltd.
High-throughput comet assay using 96 minigels.
Gutzkow, Kristine B; Langleite, Torgrim M; Meier, Silja; Graupner, Anne; Collins, Andrew R; Brunborg, Gunnar
2013-05-01
The single-cell gel electrophoresis--the comet assay--has proved to be a sensitive and relatively simple method that is much used in research for the analysis of specific types of DNA damage, and its use in genotoxicity testing is increasing. The efficiency of the comet assay, in terms of number of samples processed per experiment, has been rather poor, and both research and toxicological testing should profit from an increased throughput. We have designed and validated a format involving 96 agarose minigels supported by a hydrophilic polyester film. Using simple technology, hundreds of samples may be processed in one experiment by one person, with less time needed for processing, less use of chemicals and requiring fewer cells per sample. Controlled electrophoresis, including circulation of the electrophoresis solution, improves the homogeneity between replicate samples in the 96-minigel format. The high-throughput method described in this paper should greatly increase the overall capacity, versatility and robustness of the comet assay.
Screening for oligonucleotide binding affinity by a convenient fluorescence competition assay.
Harrison, J G; Liu, X; Balasubramanian, S
1999-09-01
A competitive homogeneous quenched fluorescence assay system is described for the high throughput screening of DNA conjugates that bind to single-stranded DNA. Fluorescence signal is generated by competitive binding of the sample molecule to a target strand labelled with a quencher probe, which is otherwise hybridised to a complementary strand containing a fluorescent probe. Thus fluorescence generated is related to the affinity of the sample. Competitive analysis of a number of peptide-oligonucleotide conjugates gave data that correlated well with the corresponding UV melting data. The assay will be useful for screening of large numbers of potential single-stranded binding molecules.
21 CFR 225.158 - Laboratory assays.
2010-04-01
... 21 Food and Drugs 4 2010-04-01 2010-04-01 false Laboratory assays. 225.158 Section 225.158 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) DRUGS... Laboratory assays. Where the results of laboratory assays of drug components, including assays by State...
Berckmans, P; Leppens, H; Vangenechten, C; Witters, H
2007-10-01
There is growing concern that some chemicals can cause endocrine disrupting effects to wild animals and humans. Therefore a rapid and reliable screening assay to assess the activity of endocrine disrupting chemicals (EDCs) is required. These EDCs can act at multiple sites. Most studied mechanism is direct interaction with the hormone receptors, e.g. estrogen receptor. In this study the luciferase reporter gene assay using transgenic human MELN cells was used. Since cytotoxicity of the chemicals can decrease the luminescent signal in the transactivation assays, a cytotoxicity assay must be implemented. Mostly the neutral red (NR) assay is performed in parallel with the estrogenicity assay. To increase the reliability and cost-efficiency of the test, a method to measure estrogenicity and cytotoxicity in the same cell culture plate instead of in parallel plates was developed and evaluated. Therefore the NR-assay was compared with the CytoTox-ONE homogeneous membrane integrity assay. The latter measures LDH (lactate dehydrogenase) leakage based on a fluorometric method. For all compounds tested, the CytoTox-ONE test showed comparable curves and EC50-values to those obtained by the NR-assay. So the CytoTox-ONE kit, which seemed more sensitive than measurements of LDH-leakage based on a colorimetric method, is recommended to test cytotoxicity to MELN cells, with the advantage to use the same cells for ER-transactivation measurements. The chemicals tested in the optimised MELN assay showed estrogenic potencies comparable to those reported for several other transactivation assays.
Kokko, Leena; Sandberg, Kaisa; Loevgren, Timo; Soukka, Tero
2004-02-09
Nanoparticles containing thousands of fluorescent europium(III) chelates have a very high specific activity compared to traditional lanthanide chelate labels. It can be assumed that if these particles are used in a homogeneous assay as donors, multiple chelates can excite a single acceptor in turns and the energy transfer to the acceptor is increased. The principle was employed in an immunoassay using luminescent resonance energy transfer from a long lifetime europium(III) chelate-dyed nanoparticle to a short lifetime, near-infrared fluorescent molecule. Due to energy transfer fluorescence lifetime of the sensitised emission was prolonged and fluorescence could be measured using a time-resolved detection. A competitive homogeneous immunoassay for estradiol was created using 92 nm europium(III) chelate-dyed nanoparticle coated with 17{beta}-estradiol specific recombinant antibody Fab fragments as a donor and estradiol conjugated with near-infrared dye AlexaFluor 680 as an acceptor. The density of Fab fragments on the surface of the particle influenced the sensitivity of the immunoassay. The optimal Fab density was reached when the entire surface of the particle participated in the energy transfer, but the areas where the energy was transferred to a single acceptor, did not overlap. We were able to detect estradiol concentrations down to 70 pmol l{sup -1} (3xSD of a standard containing 0 nmol l{sup -1} of E2) using a 96-well platform. In this study we demonstrated that nanoparticles containing lanthanide chelates could be used as efficient donors in homogeneous assays.
Development of screening assays to test novel integrin antagonists in allergic inflammation
Spartà, Antonino Maria
2009-01-01
Aim of the research: to develop a prototype of homogeneous high-throughput screening (HTS) for identification of novel integrin antagonists for the treatment of ocular allergy and to better understand the mechanisms of action of integrin-mediated levocabastine antiallergic action. Results: This thesis provides evidence that adopting scintillation proximity assay (SPA) levocabastine (IC50=406 mM), but not the first-generation antihistamine chlorpheniramine, displaces [125I]fibronectin (F...
Reichman, Melvin; Schabdach, Amanda; Kumar, Meera; Zielinski, Tom; Donover, Preston S; Laury-Kleintop, Lisa D; Lowery, Robert G
2015-12-01
Ras homologous (Rho) family GTPases act as molecular switches controlling cell growth, movement, and gene expression by cycling between inactive guanosine diphosphate (GDP)- and active guanosine triphosphate (GTP)-bound conformations. Guanine nucleotide exchange factors (GEFs) positively regulate Rho GTPases by accelerating GDP dissociation to allow formation of the active, GTP-bound complex. Rho proteins are directly involved in cancer pathways, especially cell migration and invasion, and inhibiting GEFs holds potential as a therapeutic strategy to diminish Rho-dependent oncogenesis. Methods for measuring GEF activity suitable for high-throughput screening (HTS) are limited. We developed a simple, generic biochemical assay method for measuring GEF activity based on the fact that GDP dissociation is generally the rate-limiting step in the Rho GTPase catalytic cycle, and thus addition of a GEF causes an increase in steady-state GTPase activity. We used the Transcreener GDP Assay, which relies on selective immunodetection of GDP, to measure the GEF-dependent stimulation of steady-state GTP hydrolysis by small GTPases using Dbs (Dbl's big sister) as a GEF for Cdc42, RhoA, and RhoB. The assay is well suited for HTS, with a homogenous format and far red fluorescence polarization (FP) readout, and it should be broadly applicable to diverse Rho GEF/GTPase pairs. © 2015 Society for Laboratory Automation and Screening.
Comet Assay in Cancer Chemoprevention.
Santoro, Raffaela; Ferraiuolo, Maria; Morgano, Gian Paolo; Muti, Paola; Strano, Sabrina
2016-01-01
The comet assay can be useful in monitoring DNA damage in single cells caused by exposure to genotoxic agents, such as those causing air, water, and soil pollution (e.g., pesticides, dioxins, electromagnetic fields) and chemo- and radiotherapy in cancer patients, or in the assessment of genoprotective effects of chemopreventive molecules. Therefore, it has particular importance in the fields of pharmacology and toxicology, and in both environmental and human biomonitoring. It allows the detection of single strand breaks as well as double-strand breaks and can be used in both normal and cancer cells. Here we describe the alkali method for comet assay, which allows to detect both single- and double-strand DNA breaks.
Smit, P; Neumann, H A M; Thio, H B
2012-10-01
The skin-blanching assay is used for the determination and bioequivalence of dermatologic glucocorticoids (GCs). The exact mechanism of the production of blanching is not fully understood, but it is considered that local vasoconstriction of the skin microvasculature and the consequent blood-flow reduction cause this phenomenon. Several factors influence skin blanching, including drug concentration, duration of application, nature of vehicle, occlusion, posture and location. The intensity of vasoconstriction can be measured in several ways: visual or quantitative methods, such as reflectance spectroscopy, thermography, laser Doppler velocimetry and chromametry. In literature, contradicting results in the correlation of the skin-blanching assay with different tests to determine GC sensitivity have been reported, limiting its clinical usefulness.
Bioluminescence assay for cell viability.
Lomakina, G Yu; Modestova, Yu A; Ugarova, N N
2015-06-01
Theoretical aspects of the adenosine triphosphate bioluminescence assay based on the use of the firefly luciferin-luciferase system are considered, as well as its application for assessing cell viability in microbiology, sanitation, medicine, and ecology. Various approaches for the analysis of individual or mixed cultures of microorganisms are presented, and capabilities of the method for investigation of biological processes in live cells including necrosis, apoptosis, as well as for investigation of the dynamics of metabolism are described.
Tidal spin down rates of homogeneous triaxial viscoelastic bodies
Quillen, Alice C.; Kueter-Young, Andrea; Frouard, Julien; Ragozzine, Darin
2016-12-01
We use numerical simulations to measure the sensitivity of the tidal spin-down rate of a homogeneous triaxial ellipsoid to its axis ratios by comparing the drift rate in orbital semimajor axis to that of a spherical body with the same mass, volume and simulated rheology. We use a mass-spring model approximating a viscoelastic body spinning around its shortest body axis, with spin aligned with orbital spin axis, and in circular orbit about a point mass. The torque or drift rate can be estimated from that predicted for a sphere with equivalent volume if multiplied by 0.5 (1 + b^4/a^4)(b/a)^{-4/3} (c/a)^{-α _c} where b/a and c/a are the body axis ratios and index αc ≈ 1.05 is consistent with the random lattice mass-spring model simulations but αc = 4/3 suggested by scaling estimates. A homogeneous body with axis ratios 0.5 and 0.8, like Haumea, has orbital semimajor axis drift rate about twice as fast as a spherical body with the same mass, volume and material properties. A simulation approximating a mostly rocky body but with 20 per cent of its mass as ice concentrated at its ends has a drift rate 10 times faster than the equivalent homogeneous rocky sphere. However, this increase in drift rate is not enough to allow Haumea's satellite, Hi'iaka, to have tidally drifted away from Haumea to its current orbital semimajor axis.
High-temperature study of defects and homogeneity in glass
Yoon, Chang Hyun
Glass frit has many useful applications in the glass and ceramic industries. Several attempts were made in this study to understand the origin of problems that generally occur when using glass frit. The effect of water/glass interactions on the rheology of glass suspension and the final properties of glass and glaze were studied. The dissolution of refractory inclusions and its influence on the bubble evolution, glass structure, and homogeneity of the resulting melt were also studied. The effects of long-term interaction of water with various frit suspensions were considered. The change in suspension rheology is associated with the ion concentration of the frit suspension, which strongly depends on the frit composition, additives, and solid content of frit suspension. Physical property and compositional variations resulted from dealkalization reactions between the frit particles and water. New investigative techniques for continuous monitoring and quantitative analysis of the dissolution of refractory inclusions in glass have been developed utilizing high-temperature microscopy with computer image analysis. The dissolution rates of refractory oxides in glass frit were measured utilizing hot-stage microscopy in the temperature range from 1050°C to 1400°C. The effects of dissolution on the structure of the final glass, were monitored by infrared spectroscopy. Homogenization of the resulting melts was studied using a Christiansen filter. It was found that melting temperature and time strongly influence the dissolution of refractory batch materials and subsequent homogenization rates, leading to large differences in final structures for glass melts and glazes which have not attained equilibrium.
Malate dehydrogenase activity in human seminal plasma and spermatozoa homogenates
Hulya Leventerler
2013-08-01
Full Text Available Purpose: Malate Dehydrogenase is an important enzyme of the Krebs cycle, most cells require this enzyme for their metabolic activity. We evaluated the Malate Dehydrogenase (NAD/NADP activity in human seminal plasma and sperm homogenates in normozoospermic, fertile and infertile males. Also glucose and fructose concentrations were determined in the seminal plasma samples. Material and Methods: Malate Dehydrogenase (NAD/NADP activity in human seminal plasma and sperm homogenates of normozoospermic and infertile males was determined by spectrophotometric method. Semen analysis was considered according to the WHO Criteria. Results: Malat Dehydrogenase-NAD value in seminal plasma (the mean ± SD, mU/ml of asthenoteratospermic (40.0±25.7 and azospermic (38.0±43.6 groups were significantly lower than normozoospermic, (93.9±52.1 males. Malat Dehydrogenase-NAD value in sperm homogenates (the mean ± SD, mU/ 20x106 sperm of teratospermic group (136.8±61.8 was significantly higher compared to the normozoospermic (87.3±26.5 males. Glucose concentration (mg/dl in asthenoteratospermic (4.0±1.4 and azospermic (15.4±6.4 groups were significantly higher than fertile (2.0±2.1 males. Also fructose concentration (mg/dl in asthenoteratospermic (706.6±143.3 and azospermic (338.1±228.2 groups were significantly high compared to the normozoospermic (184.7±124.8 group. Conclusion: Sperm may be some part of the source of Malat Dehydrogenase activity in semen. Malat Dehydrogenase activity in seminal plasma has an important role on energy metabolism of sperm. Intermediate substrates of Krebs cycle might have been produced under the control of Malat Dehydrogenase and these substrates may be important for sperm motility and male infertility. [Cukurova Med J 2013; 38(4.000: 648-658
A brief introduction to homogenization and miscellaneous applications*
Allaire Grégoire
2012-09-01
Full Text Available This paper is a set of lecture notes for a short introductory course on homogenization. It covers the basic tools of periodic homogenization (two-scale asymptotic expansions, the oscillating test function method and two-scale convergence and briefly describes the main results of the more general theory of G− or H−convergence. Several applications of the method are given: derivation of Darcy’s law for flows in porous media, derivation of the porosity model and long time behavior of a diffusion equation. Numerical agorithms for homogenization are also discussed, including multiscale finite element methods. Cet article reprend des notes de cours, d’un niveau introductif, sur l’homogénéisation. Ces notes couvrent la théorie de l’homogénéisation périodique (développements asymptotiques à deux échelles, méthode de la fonction test oscillante, convergence à deux échelles et décrivent brièvement les principaux résultats de la théorie plus générale de la G− ou H− convergence. Plusieurs applications de la méthode sont données : dérivation de la loi de Darcy pour des écoulements en milieux poreux, dérivation du modèle de double porosité et comportement en temps grand d’une équation de diffusion. Des algorithmes numériques pour l’homogénéisation sont aussi présentés, dont, en particulier, les méthodes d’éléments finis multi-échelles.
Viscous tilting and production of vorticity in homogeneous turbulence
Holzner, M.; Guala, M.; Lüthi, B.; Liberzon, A.; Nikitin, N.; Kinzelbach, W.; Tsinober, A.
2010-06-01
Viscous depletion of vorticity is an essential and well known property of turbulent flows, balancing, in the mean, the net vorticity production associated with the vortex stretching mechanism. In this letter, we, however, demonstrate that viscous effects are not restricted to a mere destruction process, but play a more complex role in vorticity dynamics that is as important as vortex stretching. Based on the results from three dimensional particle tracking velocimetry experiments and direct numerical simulation of homogeneous and quasi-isotropic turbulence, we show that the viscous term in the vorticity equation can also locally induce production of vorticity and changes of the orientation of the vorticity vector (viscous tilting).
Viscous tilting and production of vorticity in homogeneous turbulence
Holzner, M; Lüthi, B; Liberzon, A; Nikitin, N; Kinzelbach, W; Tsinober, A
2010-01-01
Viscous depletion of vorticity is an essential and well known property of turbulent flows, balancing, in the mean, the net vorticity production associated with the vortex stretching mechanism. In this letter we however demonstrate that viscous effects are not restricted to a mere destruction process, but play a more complex role in vorticity dynamics that is as important as vortex stretching. Based on results from particle tracking experiments (3D-PTV) and direct numerical simulation (DNS) of homogeneous and quasi isotropic turbulence, we show that the viscous term in the vorticity equation can also locally induce production of vorticity and changes of its orientation (viscous tilting).
Biotic homogenization can decrease landscape-scale forest multifunctionality
van der Plas, Fons; Manning, Pete; Soliveres, Santiago;
2016-01-01
-diversity and landscape-scale multifunctionality were always positive, because a high spatial turnover in species composition was closely related to a high spatial turnover in functions that were supported at high levels. Our findings have major implications for forest management and indicate that biotic homogenization...... a comprehensive pan-European dataset, including 16 ecosystem functions measured in 209 forest plots across six European countries, and performed simulations to investigate how local plot-scale richness of tree species (α-diversity) and their turnover between plots (β-diversity) are related to landscape...
Homogenized Elastic Properties of Graphene for Small Deformations
Jurica Sorić
2013-09-01
Full Text Available In this paper, we provide the quantification of the linear and non-linear elastic mechanical properties of graphene based upon the judicious combination of molecular mechanics simulation results and homogenization methods. We clarify the influence on computed results by the main model features, such as specimen size, chirality of microstructure, the effect of chosen boundary conditions (imposed displacement versus force and the corresponding plane stress transformation. The proposed approach is capable of explaining the scatter of the results for computed stresses, energy and stiffness and provides the bounds on graphene elastic properties, which are quite important in modeling and simulation of the virtual experiments on graphene-based devices.
HOMOGENIZATION OF SEMILINEAR PARABOLIC EQUATIONS IN PERFORATED DOMAINS
P.DONATO; A. NABIL
2004-01-01
This paper is devoted to the homogenization of a semilinear parabolic equation with rapidly oscillating coefficients in a domain periodically perforated by e-periodic holes of size ε. A Neumann condition is prescribed on the boundary of the holes.The presence of the holes does not allow to prove a compactness of the solutions in L2. To overcome this difficulty, the authors introduce a suitable auxiliary linear problem to which a corrector result is applied. Then, the asymptotic behaviour of the semilinear problem as e → 0 is described, and the limit equation is given.
Preparation of Hydroxyapatite Fibers by the Homogeneous Precipitation Method
无
2002-01-01
This paper discussed the preparing process of hydroxyapatite fibers which were widely used as reinforcement for biomedical materials by homogeneous precipitation method.The needle-like hydroxyapatite crystals were synthesized in an aqueous system.They were transferred from precursors-dicalcium phosphate anhydrate and octacalcium phosphate crystals.The reaction conditions were well controlled in order to obtain crystals in given morphology.The products were characterized by X-ray powder diffractometry(XRD),scanning electron microscopy (SEM) and infrared spectroscopy(IR).They were verified to be hydroxyapatite crystals with needle-like in shape.
On the homogeneous and heterogeneous nucleation of some organic compounds
Hienola, A.
2008-07-01
The conversion of a metastable phase into a thermodynamically stable phase takes place via the formation of clusters. Clusters of different sizes are formed spontaneously within the metastable mother phase, but only those larger than a certain size, called the critical size, will end up growing into a new phase. There are two types of nucleation: homogeneous, where the clusters appear in a uniform phase, and heterogeneous, when pre-existing surfaces are available and clusters form on them. The nucleation of aerosol particles from gas-phase molecules is connected not only with inorganic compounds, but also with nonvolatile organic substances found in atmosphere. The question is which ones of the myriad of organic species have the right properties and are able to participate in nucleation phenomena. This thesis discusses both homogeneous and heterogeneous nucleation, having as theoretical tool the classical nucleation theory (CNT) based on thermodynamics. Different classes of organics are investigated. The members of the first class are four dicarboxylic acids (succinic, glutaric, malonic and adipic). They can be found in both the gas and particulate phases, and represent good candidates for the aerosol formation due to their low vapor pressure and solubility. Their influence on the nucleation process has not been largely investigated in the literature and it is not fully established. The accuracy of the CNT predictions for binary water-dicarboxylic acid systems depends significantly on the good knowledge of the thermophysical properties of the organics and their aqueous solutions. A large part of the thesis is dedicated to this issue. We have shown that homogeneous and heterogeneous nucleation of succinic, glutaric and malonic acids in combination with water is unlikely to happen in atmospheric conditions. However, it seems that adipic acid could participate in the nucleation process in conditions occurring in the upper troposphere. The second class of organics is
Scalar oscillatory integrals in smooth spaces of homogeneous type
Gressman, Philip T
2012-01-01
We consider a generalization of the notion of spaces of homogeneous type, inspired by recent work of Street [21] on the multi-parameter Carnot-Caratheodory geometry, which imbues such spaces with differentiability structure. The setting allows one to formulate estimates for scalar oscillatory integrals on these spaces which are uniform and respect the underlying geometry of both the space and the phase function. As a corollary we obtain a generalization of a theorem of Bruna, Nagel, and Wainger [1] on the asymptotic behavior of scalar oscillatory integrals with smooth, convex phase of finite type.
Modeling the turbulent kinetic energy equation for compressible, homogeneous turbulence
Aupoix, B.; Blaisdell, G. A.; Reynolds, William C.; Zeman, Otto
1990-01-01
The turbulent kinetic energy transport equation, which is the basis of turbulence models, is investigated for homogeneous, compressible turbulence using direct numerical simulations performed at CTR. It is shown that the partition between dilatational and solenoidal modes is very sensitive to initial conditions for isotropic decaying turbulence but not for sheared flows. The importance of the dilatational dissipation and of the pressure-dilatation term is evidenced from simulations and a transport equation is proposed to evaluate the pressure-dilatation term evolution. This transport equation seems to work well for sheared flows but does not account for initial condition sensitivity in isotropic decay. An improved model is proposed.
Homogeneity of Danish environmental and clinical isolates of Shewanella algae
Vogel, Birte Fonnesbech; Holt, H.M.; Gerner-Smidt, P.;
2000-01-01
Danish isolates of Shewanella algae constituted by whole-cell protein profiling a very homogeneous group, and no clear distinction was seen between strains from the marine environment and strains of clinical origin. Although variation between all strains was observed by ribotyping and random...... amplified polymorphic DNA analysis, no clonal relationship between infective strains was found. From several patients, clonally identical strains of S. algae were reisolated up to 8 months after the primary isolation, indicating that the same strain may be able to maintain the infection....
Lipase-catalyzed synthesis of monoacylglycerol in a homogeneous system.
Monteiro, Julieta B; Nascimento, Maria G; Ninow, Jorge L
2003-04-01
The 1,3-regiospecifique lipase, Lipozyme IM, catalyzed the esterification of lauric acid and glycerol in a homogeneous system. To overcome the drawback of the insolubility of glycerol in hexane, which is extensively used in enzymatic synthesis, a mixture of n-hexane/tert-butanol (1:1, v/v) was used leading to a monophasic system. The conversion of lauric acid into monolaurin was 65% in 8 h, when a molar ratio of glycerol to fatty acid (5:1) was used with the fatty acid at 0.1 M, and the phenomenon of acyl migration was minimized.
Homogeneous approximation property for continuous shearlet transforms in higher dimensions
Yu Su
2016-07-01
Full Text Available Abstract This paper is concerned with the generalization of the homogeneous approximation property (HAP for a continuous shearlet transform to higher dimensions. First, we give a pointwise convergence result on the inverse shearlet transform in higher dimensions. Second, we show that every pair of admissible shearlets possess the HAP in the sense of L 2 ( R d $L^{2}(R^{d}$ . Third, we give a sufficient condition for the pointwise HAP to hold, which depends on both shearlets and functions to be reconstructed.
Polynomial solutions of quasi_homogeneous partial differential equations
无
2001-01-01
By means of a method of analytic number theory the following theorem is proved. Let p be a quasi_homogeneous linear partial differential operator with degree m, m>0, w.r.t a dilation {δ-τ}-{τ<0} given by (a-1,…, a-n). Assume that either a1,…, an are positive rational numbers or m=∑nj=1α-jα-j for some α=(α-1, …, αn)∈I{}+n-+. Then the dimension of the space of polynomial solutions of the equation p[u]=0 on R+n must be infinite.
Elliptic elements in a Weyl group: a homogeneity property
Lusztig, G
2010-01-01
Let G be a reductive group over an algebraically closed field whose characteristic is not a bad prime for G. Let w be an elliptic element of the Weyl group which has minimal length in its conjugacy class. We show that there exists a unique unipotent class X in G such that the following holds: if V is the variety of pairs consisting of an element g in X and a Borel subgroup B such that B,gBg^{-1} are in relative position w, then V is a homogeneous G-space.
Exhaust gas recirculation in a homogeneous charge compression ignition engine
Duffy, Kevin P.; Kieser, Andrew J.; Rodman, Anthony; Liechty, Michael P.; Hergart, Carl-Anders; Hardy, William L.
2008-05-27
A homogeneous charge compression ignition engine operates by injecting liquid fuel directly in a combustion chamber, and mixing the fuel with recirculated exhaust and fresh air through an auto ignition condition of the fuel. The engine includes at least one turbocharger for extracting energy from the engine exhaust and using that energy to boost intake pressure of recirculated exhaust gas and fresh air. Elevated proportions of exhaust gas recirculated to the engine are attained by throttling the fresh air inlet supply. These elevated exhaust gas recirculation rates allow the HCCI engine to be operated at higher speeds and loads rendering the HCCI engine a more viable alternative to a conventional diesel engine.
Improved flexibility with grayscale fabrication of calcium fluoride homogenizers
Brown, Jeremiah; Brakhage, Peter; Simmons, Lamarr; Mueller, Ralf
2012-03-01
High quality and highly uniform illumination is a critical component for advanced lithography systems and wafer inspection tools. Homogenizer elements fabricated in calcium fluoride have demonstrated good performance for deep UV applications. Grayscale photolithography allows for the fabrication of single-sided micro lens array (MLA) elements with excellent optical performance. The MLA offers some significant advantages over crossed cylinders fabricated using grayscale photolithography processes, including the reduction in the number of fabrication steps and the added flexibility of manufacturing noncylindrical surface geometries. This research presentation reviews the fabrication process and compares grayscale crossed cylindrical arrays and MLAs in terms of their capabilities and performance.
Homogeneous Poisson Structures on Loop Spaces of Symmetric Spaces
Doug Pickrell
2008-10-01
Full Text Available This paper is a sequel to [Caine A., Pickrell D., Int. Math. Res. Not., to appear, arXiv:0710.4484], where we studied the Hamiltonian systems which arise from the Evens-Lu construction of homogeneous Poisson structures on both compact and noncompact type symmetric spaces. In this paper we consider loop space analogues. Many of the results extend in a relatively routine way to the loop space setting, but new issues emerge. The main point of this paper is to spell out the meaning of the results, especially in the SU(2 case. Applications include integral formulas and factorizations for Toeplitz determinants.
Homogeneity of Danish environmental and clinical isolates of Shewanella algae
Vogel, Birte Fonnesbech; Holt, H.M.; Gerner-Smidt, P.
2000-01-01
Danish isolates of Shewanella algae constituted by whole-cell protein profiling a very homogeneous group, and no clear distinction was seen between strains from the marine environment and strains of clinical origin. Although variation between all strains was observed by ribotyping and random...... amplified polymorphic DNA analysis, no clonal relationship between infective strains was found. From several patients, clonally identical strains of S. algae were reisolated up to 8 months after the primary isolation, indicating that the same strain may be able to maintain the infection....
The General Theory of Homogenization A Personalized Introduction
Tartar, Luc
2010-01-01
Homogenization is not about periodicity, or Gamma-convergence, but about understanding which effective equations to use at macroscopic level, knowing which partial differential equations govern mesoscopic levels, without using probabilities (which destroy physical reality); instead, one uses various topologies of weak type, the G-convergence of Sergio Spagnolo, the H-convergence of Francois Murat and the author, and some responsible for the appearance of nonlocal effects, which many theories in continuum mechanics or physics guessed wrongly. For a better understanding of 20th century science,