WorldWideScience

Sample records for radiation hybrid lines

  1. Radiation hybrid mapping of human chromosome 18

    International Nuclear Information System (INIS)

    Francke, U.; Moon, A.J.; Chang, E.; Foellmer, B.; Strauss, B.; Haschke, A.; Chihlin Hsieh; Geigl, E.M.; Welch, S.

    1990-01-01

    The authors have generated a Chinese hamster V79/380-6 HPRT minus x human leukocyte hybrid cell line (18/V79) with chromosome 18 as the only human chromosome that is retained at high frequency without specific selection. Hybrid cells were selected in HAT medium, and 164 individual colonies were isolated. Of 110 colonies screened for human DNA by PCR amplification using a primer specific for human Alu repeats 67 (61%) were positive. These were expanded in culture for large-scale DNA preparations. Retesting expanded clones by PCR with Alu and LINE primers has revealed unique patterns of amplification products. In situ hybridization of biotin labelled total human DNA to metaphase spreads from various hybrids revealed the presence of one or more human DNA fragments integrated in hamster chromosomes. The authors have generated a resource that should allow the construction of a radiation map, to be compared with the YAC contig map also under construction in their laboratory

  2. Hybrid radiator cooling system

    Science.gov (United States)

    France, David M.; Smith, David S.; Yu, Wenhua; Routbort, Jules L.

    2016-03-15

    A method and hybrid radiator-cooling apparatus for implementing enhanced radiator-cooling are provided. The hybrid radiator-cooling apparatus includes an air-side finned surface for air cooling; an elongated vertically extending surface extending outwardly from the air-side finned surface on a downstream air-side of the hybrid radiator; and a water supply for selectively providing evaporative cooling with water flow by gravity on the elongated vertically extending surface.

  3. Construction of a map of chromosome 16 by using radiation hybrids

    International Nuclear Information System (INIS)

    Ceccherini, I.; Romeo, G.; Lawrence, S.; Morton, N.E.; Breuning, M.H.; Harris, P.C.; Himmelbauer, H.; Frischauf, A.M.; Sutherland, G.R.; Germino, G.G.; Reeders, S.T.

    1992-01-01

    A human-hamster cell hybrid carrying a single copy of chromosome 16 as the only human genetic material was irradiated with a single dose of γ-rays and then fused with a thymidine kinase-deficient hamster cell line (RJKM) to generate radiation hybrids retaining unselected fragments of this human chromosome. In two experiments, 223 hybrids were isolated in hypoxanthine/aminopterine/thymidine (HAT) medium and screened with 38 DNA probes, corresponding to anonymous DNA or gene sequences localized on chromosome 16. The most likely order and location of the 38 DNA sequences were established by multiple pairwise analysis and scaled to estimate physical distance in megabases. The order and the distances thus obtained are mostly consistent with available data on genetic and physical mapping of these markers, illustrating the usefulness of radiation hybrids for mapping

  4. Plant breeding by using radiation mutation - Development of disease tolerant lines of hotpepper by using radiation and interspecific hybridization

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Yong Su; Song, Hi Sup; Kim, Jin Kyu; Shin, In Chul [Nongwoo Seed Co., Suwon (Korea)

    2000-04-01

    To obtain disease resistant mutant lines, 6 inbred lines were hotppepers were irradiated with 250Gy of gamma ray and crossed between cultivar and wild species. 1) 4500 M{sub 1} plants were cultivated for obtaining M{sub 2} seed in 6 inbred lines of hotpeppers irradiated with 250 Gy of gamma ray. 2) Crossability was not generally existed among interspecific crosses, crossability between C. annum and C. chacoense was successful except crosses between C. annum, C. pubescens and C. eximium. 3) The embryo disected 45 days after pollination was suitable for embryo culture. 4) Hybrid plants were obtained from the embryo culture of the combination between C. annum and C. chacoense, while abnormal hybrid plants occurred from the combination between C. annum and C. baccatum. 15 refs., 4 figs., 4 tabs. (Author)

  5. Use of a human chromosome 11 radiation hybrid panel to map markers at 11q13

    International Nuclear Information System (INIS)

    Withers, D.; Richard, C. III; Meeker, T.C.; Maurer, S.; Evans, G.; Myers, R.M.; Cox, D.R.

    1990-01-01

    A human/hamster hybrid cell line containing human chromosome 11 was X-irradiated and 102-independent derivative lines were recovered. These 'radiation hybrids' contain random fragments of human chromosome 11. This radiation hybrid panel was used to score the retention of markers at band 11q13. Statistical analysis of marker co-retention patterns in the radiation hybrid panel permits a preliminary ordering and mapping of the markers used. The best order for six scored markers is: proximal - CD5 - CD20 - PGA - HST - BCL1 - SEA - distal. Additional markers are currently being scored. The six 11q13 markers above are spread over approximately 10-12 mB of DNA. The mapping data has implications for the identification of the bcl-1 gene. bcl-1 is the site of chromosome breakage in translocations associated with B lymphocytic malignancy. bcl-1 markers map at least 4 Mb away from any of four genes previously hypothesized to be activated by such translocations, thereby making them unlikely candidates for activation

  6. The Beam Line X NdFe-steel hybrid wiggler for SSRL

    International Nuclear Information System (INIS)

    Hoyer, E.; Halbach, K.; Humphries, D.; Marks, S.; Plate, D.; Shuman, D.; Karpenko, V.P.; Kulkarni, S.; Tirsell, K.G.

    1987-01-01

    A wiggler magnet with 15 periods, each 12.85 cm long, which achieves 1.40 T at a 2.1 cm gap (2.26T at 0.8 cm) has been designed and is now in fabrication at LBL. This wiggler will be the radiation source of the high intensity synchrotron radiation beam line for the Beam Line X PRT facility at SSRL. The magnet utilizes Neodymium-Iron (NdFe) material and Vanadium Permendur (steel) in the hybrid configuration to achieve simultaneously a high magnetic field and short period. Magnetic field adjustment is with a driven chain and ball screw drive system. The magnetic structure is external to an s.s. vacuum chamber which has thin walls, 0.76 mm thickness, at each pole tip for higher field operation. Magnetic design, construction details and magnetic measurements are presented

  7. Hybrid formulation of radiation transport in optically thick divertor plasmas

    Energy Technology Data Exchange (ETDEWEB)

    Rosato, J.; Marandet, Y.; Bufferand, H.; Stamm, R. [PIIM, UMR 7345 Aix-Marseille Universite / CNRS, Centre de St-Jerome, Marseille (France); Reiter, D. [IEK-4 Plasmaphysik, Forschungszentrum Juelich GmbH, Juelich (Germany)

    2016-08-15

    Kinetic Monte Carlo simulations of coupled atom-radiation transport in optically thick divertor plasmas can be computationally very demanding, in particular in ITER relevant conditions or even larger devices, e.g. for power plant divertor studies. At high (∝ 10{sup 15} cm{sup -3}) atomic densities, it can be shown that sufficiently large divertors behave in certain areas like a black body near the first resonance line of hydrogen (Lyman α). This suggests that, at least in part, the use of continuum model (radiation hydrodynamics) can be sufficiently accurate, while being less time consuming. In this work, we report on the development of a hybrid model devoted to switch automatically between a kinetic and a continuum description according to the plasma conditions. Calculations of the photo-excitation rate in a homogeneous slab are performed as an illustration. The outlined hybrid concept might be also applicable to neutral atom transport, due to mathematical analogy of transport equations for neutrals and radiation. (copyright 2016 The Authors. Contributions to Plasma Physics published by Wiley-VCH Verlag GmbH and Co. KGaA Weinheim. This)

  8. Circuit QED with hybrid metamaterial transmission lines

    Energy Technology Data Exchange (ETDEWEB)

    Ruloff, Stefan; Taketani, Bruno; Wilhelm, Frank [Theoretical Physics, Universitaet des Saarlandes, Saarbruecken (Germany)

    2016-07-01

    We're working on the theory of metamaterials providing some interesting results. The negative refraction index causes an opposite orientation of the wave vector k and the Poynting vector S of the travelling waves. Hence the metamaterial has a falling dispersion relation ∂ω(k)/∂k < 0 implying that low frequencies correspond to short wavelengths. Metamaterials are simulated by left-handed transmission lines consisting of discrete arrays of series capacitors and parallel inductors to ground. Unusual physics arises when right-and left-handed transmission lines are coupled forming a hybrid metamaterial transmission line. E.g. if a qubit is placed in front of a hybrid metamaterial transmission line terminated in an open circuit, the spontaneous emission rate is weakened or unaffected depending on the transition frequency of the qubit. Some other research interests are the general analysis of metamaterial cavities and the mode structure of hybrid metamaterial cavities for QND readout of multi-qubit operators. Especially the precise answer to the question about the definition of the mode volume of a metamaterial cavity is one of our primary goals.

  9. Hybrid adaptive radiotherapy with on-line MRI in cervix cancer IMRT

    International Nuclear Information System (INIS)

    Oh, Seungjong; Stewart, James; Moseley, Joanne; Kelly, Valerie; Lim, Karen; Xie, Jason; Fyles, Anthony; Brock, Kristy K.; Lundin, Anna; Rehbinder, Henrik; Milosevic, Michael; Jaffray, David

    2014-01-01

    Purpose: Substantial organ motion and tumor shrinkage occur during radiotherapy for cervix cancer. IMRT planning studies have shown that the quality of radiation delivery is influenced by these anatomical changes, therefore the adaptation of treatment plans may be warranted. Image guidance with off-line replanning, i.e. hybrid-adaptation, is recognized as one of the most practical adaptation strategies. In this study, we investigated the effects of soft tissue image guidance using on-line MR while varying the frequency of off-line replanning on the adaptation of cervix IMRT. Materials and method: 33 cervical cancer patients underwent planning and weekly pelvic MRI scans during radiotherapy. 5 patients of 33 were identified in a previous retrospective adaptive planning study, in which the coverage of gross tumor volume/clinical target volume (GTV/CTV) was not acceptable given single off-line IMRT replan using a 3 mm PTV margin with bone matching. These 5 patients and a randomly selected 10 patients from the remaining 28 patients, a total of 15 patients of 33, were considered in this study. Two matching methods for image guidance (bone to bone and soft tissue to dose matrix) and three frequencies of off-line replanning (none, single, and weekly) were simulated and compared with respect to target coverage (cervix, GTV, lower uterus, parametrium, upper vagina, tumor related CTV and elective lymph node CTV) and OAR sparing (bladder, bowel, rectum, and sigmoid). Cost (total process time) and benefit (target coverage) were analyzed for comparison. Results: Hybrid adaptation (image guidance with off-line replanning) significantly enhanced target coverage for both 5 difficult and 10 standard cases. Concerning image guidance, bone matching was short of delivering enough doses for 5 difficult cases even with a weekly off-line replan. Soft tissue image guidance proved successful for all cases except one when single or more frequent replans were utilized in the difficult cases

  10. Generation of auroral kilometric radiation in upper hybrid wave-lower hybrid soliton interaction

    International Nuclear Information System (INIS)

    Pottelette, R.; Dubouloz, N.; Treumann, R.A.

    1992-01-01

    Sporadic bursts of auroral kilometric radiation (AKR) associated with strong bursty electrostatic turbulence in the vicinity of the lower hybrid frequency have been frequently recorded in the AKR source region by the Viking satellite. The variation time scale of these emissions is typically 1 s, long enough for lower hybrid waves to grow to amplitudes of several hundred millivolts per meter and to evolve nonlinearly into solitons. On the basis on these observations it is suggested that formation of lower hybrid solitons may play a role in the generation of AKR. A theoretical model is proposed which is based on the direct acceleration of electrons in the combined lower hybrid soliton and upper hybrid wave fields. The solitons act as sporadic, localized antennas allowing for efficient conversions of the electrostatic energy stored in upper hybrid waves into electromagnetic radiation at a frequency above the X mode cutoff. Excitation of lower hybrid waves is due to the presence of energetic electron beams in the auroral zone found to be associated with steep plasma density gradients. Upper hybrid waves can be excited by a population of energetic electrons with loss cone distributions. The power of the electromagnetic radiation obtained is only noticeable in regions where the plasma frequency is less than the electron gyrofrequency. The theory predicts spectral power densities of the order of 10 -11 to 10 -9 W m -2 Hz -1 in the source region, in good agreement with the Viking observations. The sporadic nature of the radiation derives from lower hybrid soliton collapses which occur on ∼1-s time scales

  11. Beam Line VI REC-steel hybrid wiggler for SSRL

    International Nuclear Information System (INIS)

    Hoyer, E.; Chan, T.; Chin, J.W.G.; Halbach, K.; Kim, K.J.; Winick, H.; Yang, J.

    1983-03-01

    A wiggler magnet with 27 periods, each 7 cm long which reaches 1.21 T at a 1.2 cm gap and 1.64 T at 0.8 cm gap has been designed and is in fabrication. Installation in SPEAR is scheduled for mid 1983. This new wiggler will be the radiation source for a new high intensity synchrotron radiation beam line at SSRL. The magnet utilizes rare-earth cobalt (REC) material and steel in a hybrid configuration to achieve simultaneously a high magnetic field with a short period. The magnet is external to a thin walled variable gap stainless steel vacuum chamber which is opened to provide beam aperture of 1.8 cm gap at injection and then closed to a smaller aperture (< 1.0 cm). Five independent drive systems are provided to adjust the magnet and chamber gaps and alignment. Magnetic design, construction details and magnetic measurements are presented

  12. Yield Stability of Sorghum Hybrids and Parental Lines | Kenga ...

    African Journals Online (AJOL)

    Seventy-five sorghum hybrids and twenty parental lines were evaluated for two consecutive years at two locations. Our objective was to compare relative stability of grain yields among hybrids and parental lines. Mean grain yields and stability analysis of variance, which included linear regression coefficient (bi) and ...

  13. Radiation Effects of n-type, Low Resistivity, Spiral Silicon Drift Detector Hybrid Systems

    International Nuclear Information System (INIS)

    Chen, W.; De Geronimo, G.; Carini, G.A.; Gaskin, J.A.; Keister, J.W.; Li, S.; Li, Z.; Ramsey, B.D.; Siddons, D.P.; Smith, G.C.; Verbitskaya, E.

    2011-01-01

    We have developed a new thin-window, n-type, low-resistivity, spiral silicon drift detector (SDD) array - to be used as an extraterrestrial X-ray spectrometer (in varying environments) for NASA. To achieve low-energy response, a thin SDD entrance window was produced using a previously developed method. These thin-window devices were also produced on lower resistivity, thinner, n-type, silicon material, effectively ensuring their radiation hardness in anticipation of operation in potentially harsh radiation environments (such as found around the Jupiter system). Using the Indiana University Cyclotron Facility beam line RERS1, we irradiated a set of suitable diodes up to 5 Mrad and the latest iteration of our ASICs up to 12 Mrad. Then we irradiated two hybrid detectors consisting of newly, such-produced in-house (BNL) SDD chips bonded with ASICs with doses of 0.25 Mrad and 1 Mrad. Also we irradiated another hybrid detector consisting of previously produced (by KETEK) on n-type, high-resistivity SDD chip bonded with BNL's ASICs with a dose of 1 Mrad. The measurement results of radiated diodes (up to 5 Mrad), ASICs (up to 12 Mrad) and hybrid detectors (up to 1 Mrad) are presented here.

  14. Characterization and evaluation of rice blast resistance of Chinese indica hybrid rice parental lines

    Directory of Open Access Journals (Sweden)

    Yunyu Wu

    2017-12-01

    Full Text Available The development of resistant varieties and hybrid combinations has been the most effective and economical strategy to control blast disease caused by Magnaporthe oryzae. However, the distribution of major R genes and blast resistance characterization in hybrid rice parents has not been well investigated, resulting in their limited use in hybrid rice blast-resistance breeding. In the present study, 88 elite indica hybrid rice parental lines were evaluated with 30 isolates of M. oryzae collected from the main planting area of indica hybrid rice in China and were characterized for the presence of 11 major resistance genes using molecular markers. The pathogenicity assays showed that four types of hybrid rice parent line showed some resistance to M. oryzae. However, the proportions of highly resistant lines and the mean resistance frequency (RF varied among the four types, with resistance in decreasing order shown by three-line restorer lines, three-line maintainer lines, two-line sterile lines, and two-line restorer lines. All 88 hybrid rice parental lines carried more than one R gene, but none carried the R genes Pi1 and Pi2. Although Pid3 and Pi9 were present only in three-line restorer lines and Pigm only in three-line maintainer lines, the remaining six R genes (Pib, Pid2, Pi5, Pia, Pi54, and Pita were present in the four types of hybrid rice parent with significantly different distribution frequencies. The correlation between R genes and resistance reactions was investigated. The results are expected to provide useful information for rational utilization of major R genes in hybrid rice breeding programs. Keywords: Hybrid rice parental lines, Magnaporthe oryzae, Pi genes, Resistance evaluation, Molecular markers

  15. Combining ability of tomato lines in saladette-type hybrids

    Directory of Open Access Journals (Sweden)

    Marcela Carvalho Andrade

    2014-09-01

    Full Text Available Given the growing importance of the saladette fresh tomato market in Brazil, the objective of this paper was to assess the combining abilities of lines potentially useful as parents of hybridsin this class. The experiment consisted of28 genotypes, 18 hybrids from a partial diallel crossobtained from crossing two groups of tomato lines (Group I, with 9 parents, and Group II, with 2 parents, 8 F1 experimental hybrids, and 2 commercial checks. Traits evaluated were total yield, mean fruit mass, fruit shelf life, shape and percentsoluble solids. Additive genetic effects were generally more important than non-additive effects for all traits evaluated. The TOM-542 and TOM-734 lines, from group I, and the TOM-720 line, from group II, presented high general combining ability (GCA estimates for most of the traits of importance for saladette tomatoes, and were therefore considered suitable parents of hybrids of this class. Higher fruit shelf life of TOM-723 as a parental line compared with TOM-720 (Group II, was mainly attributed to the presence in the former of the norA allele, which controls longer fruit shelf life. F1 hybrids (TOM-542 x TOM-720, (TOM-580 x TOM-720, (TOM-734 x TOM-720, and (TOM-727 x TOM-720 showed good performance and fruit quality and thus constitute possible commercial varieties.

  16. Development of Elite BPH-Resistant Wide-Spectrum Restorer Lines for Three and Two Line Hybrid Rice.

    Science.gov (United States)

    Fan, Fengfeng; Li, Nengwu; Chen, Yunping; Liu, Xingdan; Sun, Heng; Wang, Jie; He, Guangcun; Zhu, Yingguo; Li, Shaoqing

    2017-01-01

    Hybrid rice has contributed significantly to the world food security. Breeding of elite high-yield, strong-resistant broad-spectrum restorer line is an important strategy for hybrid rice in commercial breeding programs. Here, we developed three elite brown planthopper (BPH)-resistant wide-spectrum restorer lines by pyramiding big-panicle gene Gn8.1 , BPH-resistant genes Bph6 and Bph9 , fertility restorer genes Rf3, Rf4, Rf5 , and Rf6 through molecular marker assisted selection. Resistance analysis revealed that the newly developed restorer lines showed stronger BPH-resistance than any of the single-gene donor parent Luoyang-6 and Luoyang-9. Moreover, the three new restorer lines had broad spectrum recovery capabilities for Honglian CMS, Wild abortive CMS and two-line GMS sterile lines, and higher grain yields than that of the recurrent parent 9,311 under nature field conditions. Importantly, the hybrid crosses also showed good performance for grain yield and BPH-resistance. Thus, the development of elite BPH-resistant wide-spectrum restorer lines has a promising future for breeding of broad spectrum BPH-resistant high-yield varieties.

  17. New hybrid conjugate gradient methods with the generalized Wolfe line search.

    Science.gov (United States)

    Xu, Xiao; Kong, Fan-Yu

    2016-01-01

    The conjugate gradient method was an efficient technique for solving the unconstrained optimization problem. In this paper, we made a linear combination with parameters β k of the DY method and the HS method, and putted forward the hybrid method of DY and HS. We also proposed the hybrid of FR and PRP by the same mean. Additionally, to present the two hybrid methods, we promoted the Wolfe line search respectively to compute the step size α k of the two hybrid methods. With the new Wolfe line search, the two hybrid methods had descent property and global convergence property of the two hybrid methods that can also be proved.

  18. Line radiative transfer and statistical equilibrium*

    Directory of Open Access Journals (Sweden)

    Kamp Inga

    2015-01-01

    Full Text Available Atomic and molecular line emission from protoplanetary disks contains key information of their detailed physical and chemical structures. To unravel those structures, we need to understand line radiative transfer in dusty media and the statistical equilibrium, especially of molecules. I describe here the basic principles of statistical equilibrium and illustrate them through the two-level atom. In a second part, the fundamentals of line radiative transfer are introduced along with the various broadening mechanisms. I explain general solution methods with their drawbacks and also specific difficulties encountered in solving the line radiative transfer equation in disks (e.g. velocity gradients. I am closing with a few special cases of line emission from disks: Radiative pumping, masers and resonance scattering.

  19. Evaluation on reaction of late maturing maize hybrids and lines to Fusarium ear rot.

    Directory of Open Access Journals (Sweden)

    M. Haddadi

    2015-03-01

    Full Text Available Abstract. In order to evaluate and determine resistance rates of different corn genotypes to Fusarium ear rot, 22 inbred lines and 19 late and medium maturity hybrids in 2009 and 17 inbred lines and 14 late and medium maturity hybrids were planted in Qarakheil Agricultural Research Station in 2010. Each line and hybrid were planted separately. For each experiment a randomized complete block design with three replications was used. Plant ears were inoculated by Nail th Punch method at the 10 day after anthesis. When the disease symptoms were observed, evaluation of each line and genotype was done based on percentage and severity of the disease symptom. The result in 2009 showed that 14 hybrids were tolerant. Hybrids of K3640/3 X MO17, K166B X K18, K166B X K19/1 and K3547/4 X MO17 were resistant. One hybrid was susceptible. Pure lines of K18 and K LM77007/7-2-6-3-1-2-1 were resistant. 14 tolerance lines and 6 susceptible lines were shown. In 2010 hybrids of K166B X K18 and K3653/2 X K18 were resistant. The other hybrids were tolerant. Pure lines of K3547/3 and K18 were resistant. Five tolerance lines were also shown.

  20. Radiation sterilization of enzyme hybrids with biodegradable polymers

    International Nuclear Information System (INIS)

    Furuta, Masakazu; Oka, Masahito; Hayashi, Toshio

    2002-01-01

    Ionizing radiations, which have already been utilized for the sterilization of medical supplies as well as gas fumigation, should be the final candidate to decontaminate 'hybrid' biomaterials containing bio-active materials including enzymes because irradiation induces neither heat nor substances affecting the quality of the materials and our health. In order to check the feasibility of 60 Co-gamma rays on these materials, we selected commercial proteases including papain and bromelain hybridized with commercial activated chitosan beads and demonstrated that these enzyme-hybrids suspended in water showed the significant radiation durability of more than twice as much as free enzyme solution at 25-kGy irradiation. Enhanced thermal and storage stability of the enzyme hybrids were not affected by the same dose level of irradiation, either, indicating that commercial irradiation sterilization method is applicable to enzyme hybrids without modification

  1. Improving restorer line of hybrid rice by irradiation

    International Nuclear Information System (INIS)

    Guo Guangrong; Yi Weiping; Liu Wuquan

    1995-03-01

    The work for improving restorer line of hybrid rice has been done. The results showed the radiosensitivity of foreign varieties overtakes Chinese ones at average level. Because of their different blood relationship, there are various situation on foreign varieties, i.e. varieties from IR system are not sensitive, Shui-yun system are second and Miyang system are sensitive. The radiosensitivity for restorer lines of hybrid F 0 overtakes one for F 1 . According to this results. We have put forward the point of view 'Multi-gene type blend system'. M 2 mutant frequency of restorer line was investigated. The results showed: there was a little difference between the total mutant frequencies from the different varieties. But, there may be difference in some characters by over thirty times between them. So a problem, worthy to be further studied is proposed that do the differences of radiosensitivity between varieties relate to the mutant frequency of these characters? Various mutants were obtained by irradiation treatment, among which a few mutants changed to maintainer line because losing restorer genes, other more mutants still were restorer lines. New combinations developed by these new mutant restorer lines have strong heterosis. The optimum combinations have been utilized in rice production. (7 tabs.)

  2. Small-Signal Analysis of Autonomous Hybrid Distributed Generation Systems in Presence of Ultracapacitor and Tie-Line Operation

    Science.gov (United States)

    Ray, Prakash K.; Mohanty, Soumya R.; Kishor, Nand

    2010-07-01

    This paper presents small-signal analysis of isolated as well as interconnected autonomous hybrid distributed generation system for sudden variation in load demand, wind speed and solar radiation. The hybrid systems comprise of different renewable energy resources such as wind, photovoltaic (PV) fuel cell (FC) and diesel engine generator (DEG) along with the energy storage devices such as flywheel energy storage system (FESS) and battery energy storage system (BESS). Further ultracapacitors (UC) as an alternative energy storage element and interconnection of hybrid systems through tie-line is incorporated into the system for improved performance. A comparative assessment of deviation of frequency profile for different hybrid systems in the presence of different storage system combinations is carried out graphically as well as in terms of the performance index (PI), ie integral square error (ISE). Both qualitative and quantitative analysis reflects the improvements of the deviation in frequency profiles in the presence of the ultracapacitors (UC) as compared to other energy storage elements.

  3. Radiation protection during hybrid procedures: innovation creates new challenges.

    Science.gov (United States)

    Sawdy, Jaclynn M; Gocha, Mark D; Olshove, Vincent; Chisolm, Joanne L; Hill, Sharon L; Phillips, Alistair; Galantowicz, Mark; Cheatham, John P; Holzer, Ralf J

    2009-09-01

    The cooperation between interventional cardiologists and cardiothoracic surgeons has expanded the spectrum of treatment modalities for patients with congenital heart disease. These hybrid techniques have created new challenges, one of which being the provision of adequate but practical radiation protection. This study evaluates the use of a lightweight radiation protection drape (RADPAD) that may be suitable for shielding during hybrid procedures. To simulate a pediatric patient, an 8.7 liter water-filled tub was placed on an X-ray table and exposed to 10-second cine acquisition runs. Radiation exposure was measured at twelve specified locations around the table using a model with three different levels of radiation protection: no shielding, shielding using a traditional 0.35 mm lead-equivalent apron, and shielding using the 0.25 mm lead-equivalent RADPAD. The traditional lead apron and the RADPAD significantly reduced the amount of radiation dose when compared with no shielding. The standard lead apron provided slightly greater radiation protection than the RADPAD (0.000064 radiation absorbed dose [rad] vs. 0.000091 rad; p = 0.012). The measured rad was significantly higher on the right side of the table, and the measured radiation dose decreased significantly with increasing distance from the table. The RADPAD has been shown to function as an efficient shielding device, even though it does not quite match the protection that can be expected from a standard lead apron. It complies with regulatory radiation protection requirements and its lightweight and sterile use make it particularly useful during hybrid procedures in the operating room.

  4. Charmonium meson and hybrid radiative transitions

    Energy Technology Data Exchange (ETDEWEB)

    Guo, Peng [Indiana U., JLAB; Yépez-Martínez, Tochtli [Indiana U.; Szczepaniak, Adam P. [Indiana U., JLAB

    2014-06-01

    We consider the non-relativistic limit of the QCD Hamiltonian in the Coulomb gauge, to describe radiative transitions between conventional charmonium states and from the lowest multiplet of cc¯ hybrids to charmonium mesons. The results are compared to potential quark models and lattices calculations.

  5. Radiation resistant lining material

    International Nuclear Information System (INIS)

    Ouchi, Koki; Okagawa, Seigo; Tamaki, Hidehiro.

    1990-01-01

    Rigidity, viscoelasticity, flexibility, radiation resistance, leaching resistance, rust-proofness, endurance, etc. are required for the lining materials to wall surfaces and floor surfaces of facilities used under the effect of radiation rays and for the inner surface protection of vessels for radioactive wastes. The present invention provides radiation resistant lining material capable of satisfying such various requirements in a well-balanced manner. That is, the material contains (A) 100 parts by weight of rapidly curing cement, (B) 50 to 300 % by weight of aggregate, and (C) 80 to 120 parts by weight of polymer emulsion. As the specific example, the ingredient (A) is commercially available under the trade name of Jet Cement. The aggregate of the ingredient (B) has preferably from about 0.6 to 0.2 mm of size and is made of material, preferably, silicon or iron grains. As the ingredient (C), acrylic resin emulsion is preferred. As a result of example, these ingredient constitutions can satisfy each of the required performance described above. (I.S.)

  6. Radiation sensitivity of human lung cancer cell lines

    International Nuclear Information System (INIS)

    Carmichael, J.; Degraff, W.G.; Gamson, J.; Russo, G.; Mitchell, J.B.; Gazdar, A.F.; Minna, J.D.; Levitt, M.L.

    1989-01-01

    X-Ray survival curves were determined using a panel of 17 human lung cancer cell lines, with emphasis on non-small cell lung cancer (NSCLC). In contrast to classic small cell lung cancer (SCLC) cell lines, NSCLC cell lines were generally less sensitive to radiation as evidenced by higher radiation survival curve extrapolation numbers, surviving fraction values following a 2Gy dose (SF2) and the mean inactivation dose values (D) values. The spectrum of in vitro radiation responses observed was similar to that expected in clinical practice, although mesothelioma was unexpectedly sensitive in vitro. Differences in radiosensitivity were best distinguished by comparison of SF2 values. Some NSCLC lines were relatively sensitive, and in view of this demonstrable variability in radiation sensitivity, the SF2 value may be useful for in vitro predictive assay testing of clinical specimens. (author)

  7. Hybrid anode for semiconductor radiation detectors

    Science.gov (United States)

    Yang, Ge; Bolotnikov, Aleksey E; Camarda, Guiseppe; Cui, Yonggang; Hossain, Anwar; Kim, Ki Hyun; James, Ralph B

    2013-11-19

    The present invention relates to a novel hybrid anode configuration for a radiation detector that effectively reduces the edge effect of surface defects on the internal electric field in compound semiconductor detectors by focusing the internal electric field of the detector and redirecting drifting carriers away from the side surfaces of the semiconductor toward the collection electrode(s).

  8. Line radiative transfer and statistical equilibrium

    NARCIS (Netherlands)

    Kamp, Inga

    Atomic and molecular line emission from protoplanetary disks contains key information of their detailed physical and chemical structures. To unravel those structures, we need to understand line radiative transfer in dusty media and the statistical equilibrium, especially of molecules. I describe

  9. Radiation hardness assessment of the charge-integrating hybrid pixel detector JUNGFRAU 1.0 for photon science

    Energy Technology Data Exchange (ETDEWEB)

    Jungmann-Smith, J. H., E-mail: jsmith@magnet.fsu.edu; Bergamaschi, A.; Brückner, M.; Dinapoli, R.; Greiffenberg, D.; Jaggi, A.; Maliakal, D.; Mayilyan, D.; Mezza, D.; Mozzanica, A.; Ramilli, M.; Ruder, Ch.; Schädler, L.; Schmitt, B.; Shi, X.; Tinti, G. [Paul Scherrer Institute, 5232 Villigen PSI (Switzerland); Cartier, S. [Paul Scherrer Institute, 5232 Villigen PSI (Switzerland); Institute for Biomedical Engineering, University and ETHZ, 8092 Zürich (Switzerland); Medjoubi, K. [Synchrotron Soleil, L’Orme des Merisiers, Saint-Aubin–BP 48, 91192 GIF-sur-Yvette Cedex (France)

    2015-12-15

    JUNGFRAU (adJUstiNg Gain detector FoR the Aramis User station) is a two-dimensional hybrid pixel detector for photon science applications in free electron lasers, particularly SwissFEL, and synchrotron light sources. JUNGFRAU is an automatic gain switching, charge-integrating detector which covers a dynamic range of more than 10{sup 4} photons of an energy of 12 keV with a good linearity, uniformity of response, and spatial resolving power. The JUNGFRAU 1.0 application-specific integrated circuit (ASIC) features a 256 × 256 pixel matrix of 75 × 75 μm{sup 2} pixels and is bump-bonded to a 320 μm thick Si sensor. Modules of 2 × 4 chips cover an area of about 4 × 8 cm{sup 2}. Readout rates in excess of 2 kHz enable linear count rate capabilities of 20 MHz (at 12 keV) and 50 MHz (at 5 keV). The tolerance of JUNGFRAU to radiation is a key issue to guarantee several years of operation at free electron lasers and synchrotrons. The radiation hardness of JUNGFRAU 1.0 is tested with synchrotron radiation up to 10 MGy of delivered dose. The effect of radiation-induced changes on the noise, baseline, gain, and gain switching is evaluated post-irradiation for both the ASIC and the hybridized assembly. The bare JUNGFRAU 1.0 chip can withstand doses as high as 10 MGy with minor changes to its noise and a reduction in the preamplifier gain. The hybridized assembly, in particular the sensor, is affected by the photon irradiation which mainly shows as an increase in the leakage current. Self-healing of the system is investigated during a period of 11 weeks after the delivery of the radiation dose. Annealing radiation-induced changes by bake-out at 100 °C is investigated. It is concluded that the JUNGFRAU 1.0 pixel is sufficiently radiation-hard for its envisioned applications at SwissFEL and synchrotron beam lines.

  10. Study of improvement of indices maize line to establish their position in hybrids

    Directory of Open Access Journals (Sweden)

    Oxana DIRZU-COCOS

    2015-12-01

    Full Text Available Corn (Zea mays L. crop that is grown on large areas - over 140 million hectares worldwide, and 400-500 thousand ha in Moldova due to production potential broad diversity of use as food for humans, animals, birds raw material for industrial processing. The upward trend in average yields achieved is largely attributed to the improvement of scientific programs. Select the line with the characters and traits that are transmitted hereditary hybrids and contribute to their performance, ensure progress in improvement. Therefore, the process of creating inbred lines associated with combining ability testing as a measure of productivity conferred hybrids, is significant research programs. Orientation purpose of improved maize hybrids to formulas and simple change to a superior capitalization heterosis effect and perfect uniformity of plant requires changes in methodology for the creation, evaluation and classification of inbred lines.

  11. Zebrafish syntenic relationship to human/mouse genomes revealed by radiation hybrid mapping

    International Nuclear Information System (INIS)

    Samonte, Irene E.

    2007-01-01

    Zebrafish (Danio rerio) is an excellent model system for vertebrate developmental analysis and a new model for human disorders. In this study, however, zebrafish was used to determine its syntenic relationship to human/mouse genomes using the zebrafish-hamster radiation hybrid panel. The focus was on genes residing on chromosomes 6 and 17 of human and mouse, respectively, and some other genes of either immunologic or evolutionary importance. Gene sequences of interest and zebrafish expressed sequence tags deposited in the GenBank were used in identifying zebrafish homologs. Polymerase chain reaction (PCR) amplification, cloning and subcloning, sequencing, and phylogenetic analysis were done to confirm the homology of the candidate genes in zebrafish. The promising markers were then tested in the 94 zebrafish-hamster radiation hybrid panel cell lines and submitted for logarithm of the odds (LOD) score analysis to position genes on the zebrafish map. A total of 19 loci were successfully mapped to zebrafish linkage groups 1, 14, 15, 19, and 20. Four of these loci were positioned in linkage group 20, whereas, 3 more loci were added in linkage group 19, thus increasing to 34 loci the number of human genes syntenic to the group. With the sequencing of the zebrafish genome, about 20 more MHC genes were reported linked on the same group. (Author)

  12. On-line identification of hybrid systems using an adaptive growing and pruning RBF neural network

    DEFF Research Database (Denmark)

    Alizadeh, Tohid

    2008-01-01

    This paper introduces an adaptive growing and pruning radial basis function (GAP-RBF) neural network for on-line identification of hybrid systems. The main idea is to identify a global nonlinear model that can predict the continuous outputs of hybrid systems. In the proposed approach, GAP......-RBF neural network uses a modified unscented kalman filter (UKF) with forgetting factor scheme as the required on-line learning algorithm. The effectiveness of the resulting identification approach is tested and evaluated on a simulated benchmark hybrid system....

  13. Natural radiation focused by power lines: new evidence

    Energy Technology Data Exchange (ETDEWEB)

    Hopwood, Anthony

    1992-11-01

    Scientists searching for a mechanism to explain increases in the incidence of cancer among those living in close proximity to power lines could have been looking in the wrong place. New evidence suggests that instead of trying to find an as yet unproven cellular reaction to the presence of the power-line's magnetic fields, researchers should investigate power lines as concentrators of potentially damaging natural sky radiation. If accepted, a clear link between a known biological cell damage mechanism and power lines will have been established, triggering a reassessment of the independent studies recording statistical increases in cancer incidence around power lines. The evidence stems from recordings showing concentrations of background solar radiation under power lines - a direction of enquiry prompted by a chance observation made during a British Astronomical Association experiment. (Author).

  14. Behavior of hybrid concentrated photovoltaic-thermoelectric generator under variable solar radiation

    DEFF Research Database (Denmark)

    Mahmoudi Nezhad, Sajjad; Rezaniakolaei, Alireza; Rosendahl, Lasse Aistrup

    2018-01-01

    diversely versus changing the solar radiation and module temperature. Moreover, the thermal response of the TEG stabilizes temperature fluctuation of the hybrid module when the solar radiation rapidly changes. In this work, impact of the thermal contact resistance on the temperature profile and system...... and solved by finite volume algorithm. In spite of temperatures profile in the hybrid CPV-TEG module, as results of variation of solar irradiation, power generation and efficiency of the CPV and TEG under the transient condition are presented. The results show that efficiency of the TEG and CPV varies...

  15. A High Resolution Radiation Hybrid Map of Wheat Chromosome 4A

    Czech Academy of Sciences Publication Activity Database

    Balcárková, Barbora; Frenkel, Z.; Škopová, Monika; Abrouk, Michael; Kumar, A.; Chao, S.; Kianian, S. F.; Akhunov, E.; Korol, A.; Doležel, Jaroslav; Valárik, Miroslav

    2017-01-01

    Roč. 7, JAN 10 (2017), č. článku 2063. ISSN 1664-462X R&D Projects: GA MŠk(CZ) LO1204; GA ČR(CZ) GA14-07164S Institutional support: RVO:61389030 Keywords : triticum-aestivum l. * bread wheat * high-density * agronomic traits * tetraploid wheat * hexaploid wheat * polyploid wheat * genetic maps * genomes * recombination * endosperm radiation hybrid panel * radiation hybrid map * wheat chromosome 4A * chromosome deletion bin map * Triticum aestivum * SNP iSelect array Subject RIV: EB - Genetics ; Molecular Biology OBOR OECD: Plant sciences, botany Impact factor: 4.298, year: 2016

  16. Correlation between ultraviolet survival and DNA repair efficiency in mouse cell hybrids and their parent lines

    International Nuclear Information System (INIS)

    Limbosch, S.

    1982-01-01

    Three hybrid cell lines formed between mouse lymphoma (LS) and mouse fibroblasts (A9) have been tested for their capacity to perform unscheduled DNA synthesis; their recovery characteristics after uv irradiation have also been studied to determine if DNA repair is implicated in the high survival observed in one hybrid (clone 3). The results of these investigations indicate that hybrid clone 3 was distinguishable from the more uv sensitive parental and other hybrid cell lines by its higher uv-induced unscheduled DNA synthesis, its greater clonogenic survival in plateau phase, and its faster recovery when maintained in conditioned medium after irradiation. The simultaneous increase of these three properties in hybrid clone 3 suggest that, by three different approaches, we have evidenced the same molecular process, a process involved in the elimination of potentially lethal damage, most probably the excision repair pathway. This report also shows that the low efficiency in excision repair in the parent line A9 is probably not due to deletion but rather to repression of the relevant gene(s) and that somatic cell hybridization can result in a stimulation of a previously poorly expressed repair process

  17. Performance Analysis of GFDL's GCM Line-By-Line Radiative Transfer Model on GPU and MIC Architectures

    Science.gov (United States)

    Menzel, R.; Paynter, D.; Jones, A. L.

    2017-12-01

    Due to their relatively low computational cost, radiative transfer models in global climate models (GCMs) run on traditional CPU architectures generally consist of shortwave and longwave parameterizations over a small number of wavelength bands. With the rise of newer GPU and MIC architectures, however, the performance of high resolution line-by-line radiative transfer models may soon approach those of the physical parameterizations currently employed in GCMs. Here we present an analysis of the current performance of a new line-by-line radiative transfer model currently under development at GFDL. Although originally designed to specifically exploit GPU architectures through the use of CUDA, the radiative transfer model has recently been extended to include OpenMP in an effort to also effectively target MIC architectures such as Intel's Xeon Phi. Using input data provided by the upcoming Radiative Forcing Model Intercomparison Project (RFMIP, as part of CMIP 6), we compare model results and performance data for various model configurations and spectral resolutions run on both GPU and Intel Knights Landing architectures to analogous runs of the standard Oxford Reference Forward Model on traditional CPUs.

  18. Two-line hybrid rice male sterile line 'NHR111S' with a marker of green-revertible albino leaves

    International Nuclear Information System (INIS)

    Wu Wei; Liu Xin; Shu Xiaoli; Shu Qingyao; Xia Yingwu; Wu Dianxing

    2006-01-01

    NHR111S is a new two-line male sterile line with a marker of green-revertible albino leave that was bred from in vitro mutagens is of the thermo/photoperiod-sensitive male sterile line 'Guangzhan63S' by 60 Co γ-rays. It has the same desired agronomic traits, fertility characteristics and combining ability as characteristics of the parent. It is convenient to develop leaf color marker-aided elimination strategy in the multiplication and production of hybrid rice seeds. (authors)

  19. On-line radiation teaching materials using IT technology

    International Nuclear Information System (INIS)

    Inoue, Hiroyoshi

    2005-01-01

    We developed the on-line radiation teaching materials using the Internet, in order to provide the teaching support materials of atomic power and radiation educations in on-school study, as well as to create the complementary study system in off-school study. The themes of teaching materials were selected from requests by teachers. In the case of an elementary school, the teaching material 'an environmental problem and atomic power' was created as the aggregate of each content for study without boundary between subjects. The teaching material 'medical treatment and radiation' was created for junior high school students to raise the individual knowledge. In the case of a high school, the teaching material nucleus and radiation' was prepared to supplement the physical study of students. The on-line teaching materials were tried to 300 junior high school and high school students, 68% of students answered that the teaching material is effective to understand atomic power and radiation, though 17% answered they were not effective. Although there are problems to prepare IT learning equipments and learning follow-up system in the material, it is suggested that the on-line teaching materials will provide the novel learning system including debates for the study. This method has no limitation of time and place. (author)

  20. Effect of radiation on fruit pollen germination and distant hybridization compatibility

    International Nuclear Information System (INIS)

    Li Jing; Shang Xiaoli

    2006-01-01

    Pollens of Zhouxingshantao peach trees, apricot cultivar Katy and plum cultivar Friar were irradiated by different doses of 60 Co γ-rays and ultraviolet to study the radiation effect on the pollen germination and distant hybridization settings. The germination percentages of the pollen irradiated by 60 Co γ-rays and ultraviolet were lower than those of the controls. The pollens of the tested fruits have different sensitivities of 60 Co γ-rays and ultraviolet: the Friar pollen was the most sensitive to the radiation, and the Katy was the least. With the germinate percentages of the irradiated pollen dropping, the distant hybridization fruit setting percentage also lowered. (authors)

  1. Accelerated line-by-line calculations for the radiative transfer of trace gases related to climate studies

    International Nuclear Information System (INIS)

    Clough, S.A.

    1993-01-01

    In the present study we are studying the effects of including carbon dioxide, ozone, methane, and the halocarbons in addition to water vapor in the radiating atmosphere. The study has focused on two principal issues: the effect on the spectral fluxes and cooling rates of carbon dioxide, ozone and the halocarbons at 1990 concentration levels and the change in fluxes and cooling rates as a consequence of the anticipated ten year change in the profiles of these species. For the latter study the water vapor profiles have been taken as invariant in time. The radiative line-by-line calculations using LBLRTM (Line-By-Line Radiative Transfer Model) have been performed for tropical (TRP), mid-latitude winter (MLW) and mid-latitude summer (MLS) model atmospheres. The halocarbons considered in the present study are CCl 4 , CFC-11, CFC-12 and CFC-22. In addition to considering the radiative effects of carbon dioxide at 355 ppM, the assumed current level, we have also obtained results for doubled carbon dioxide at 710 ppM. An important focus of the current research effort is the effect of the ozone depletion profile on atmospheric radiative effects

  2. Non-LTE line-blanketed model atmospheres of hot stars. 1: Hybrid complete linearization/accelerated lambda iteration method

    Science.gov (United States)

    Hubeny, I.; Lanz, T.

    1995-01-01

    A new munerical method for computing non-Local Thermodynamic Equilibrium (non-LTE) model stellar atmospheres is presented. The method, called the hybird complete linearization/accelerated lambda iretation (CL/ALI) method, combines advantages of both its constituents. Its rate of convergence is virtually as high as for the standard CL method, while the computer time per iteration is almost as low as for the standard ALI method. The method is formulated as the standard complete lineariation, the only difference being that the radiation intensity at selected frequency points is not explicity linearized; instead, it is treated by means of the ALI approach. The scheme offers a wide spectrum of options, ranging from the full CL to the full ALI method. We deonstrate that the method works optimally if the majority of frequency points are treated in the ALI mode, while the radiation intensity at a few (typically two to 30) frequency points is explicity linearized. We show how this method can be applied to calculate metal line-blanketed non-LTE model atmospheres, by using the idea of 'superlevels' and 'superlines' introduced originally by Anderson (1989). We calculate several illustrative models taking into accont several tens of thosands of lines of Fe III to Fe IV and show that the hybrid CL/ALI method provides a robust method for calculating non-LTE line-blanketed model atmospheres for a wide range of stellar parameters. The results for individual stellar types will be presented in subsequent papers in this series.

  3. Evaluation of new hybrid brachiaria lines in Thailand. 1. Forage production and quality

    Directory of Open Access Journals (Sweden)

    Michael D. Hare

    2015-05-01

    Full Text Available Forty-three new hybrid bracharia lines were evaluated for forage accumulation and nutritive value in Northeast Thailand from 2006 to 2011 in experiments at 2 sites, using Mulato II hybrid brachiaria as a standard for comparison. The parameters evaluated were wet and dry season dry matter (DM accumulation, leaf:stem ratio, crude protein (CP concentration and fiber level [acid detergent fiber (ADF and neutral detergent fiber (NDF]. No lines consistently displayed superior dry season forage accumulation and leaf:stem ratio over Mulato II. In the wet seasons, 14 lines produced more DM than Mulato II but in only one wet season each. Mulato II produced forage with high leaf:stem ratio in all seasons. Many lines did have significantly higher CP concentrations and lower levels of ADF and NDF than Mulato II, but their forage accumulation and leaf:stem ratio were inferior. Four lines (BR02/1718, BR02/1752, BR02/1794 and BR02/0465 were granted Plant Variety Rights in 2011.Keywords: Cayman, Cobra, crude protein, dry matter yields, forage regrowth,  Mulato II.DOI: 10.17138/TGFT(383-93 

  4. Line-Enhanced Deformable Registration of Pulmonary Computed Tomography Images Before and After Radiation Therapy With Radiation-Induced Fibrosis

    Science.gov (United States)

    Sensakovic, William F.; Maxim, Peter; Diehn, Maximilian; Loo, Billy W.; Xing, Lei

    2018-01-01

    Purpose: The deformable registration of pulmonary computed tomography images before and after radiation therapy is challenging due to anatomic changes from radiation fibrosis. We hypothesize that a line-enhanced registration algorithm can reduce landmark error over the entire lung, including the irradiated regions, when compared to an intensity-based deformable registration algorithm. Materials: Two intensity-based B-spline deformable registration algorithms of pre-radiation therapy and post-radiation therapy images were compared. The first was a control intensity–based algorithm that utilized computed tomography images without modification. The second was a line enhancement algorithm that incorporated a Hessian-based line enhancement filter prior to deformable image registration. Registrations were evaluated based on the landmark error between user-identified landmark pairs and the overlap ratio. Results: Twenty-one patients with pre-radiation therapy and post-radiation therapy scans were included. The median time interval between scans was 1.2 years (range: 0.3-3.3 years). Median landmark errors for the line enhancement algorithm were significantly lower than those for the control algorithm over the entire lung (1.67 vs 1.83 mm; P 5 Gy (2.25 vs 3.31; P 5 Gy dose interval demonstrated a significant inverse relationship with post-radiation therapy fibrosis enhancement after line enhancement filtration (Pearson correlation coefficient = −0.48; P = .03). Conclusion: The line enhancement registration algorithm is a promising method for registering images before and after radiation therapy. PMID:29343206

  5. Study on hybrid multi-objective optimization algorithm for inverse treatment planning of radiation therapy

    International Nuclear Information System (INIS)

    Li Guoli; Song Gang; Wu Yican

    2007-01-01

    Inverse treatment planning for radiation therapy is a multi-objective optimization process. The hybrid multi-objective optimization algorithm is studied by combining the simulated annealing(SA) and genetic algorithm(GA). Test functions are used to analyze the efficiency of algorithms. The hybrid multi-objective optimization SA algorithm, which displacement is based on the evolutionary strategy of GA: crossover and mutation, is implemented in inverse planning of external beam radiation therapy by using two kinds of objective functions, namely the average dose distribution based and the hybrid dose-volume constraints based objective functions. The test calculations demonstrate that excellent converge speed can be achieved. (authors)

  6. Simulations of hybrid system varying solar radiation and microturbine response time

    Directory of Open Access Journals (Sweden)

    Yolanda Fernández Ribaya

    2015-07-01

    Full Text Available Hybrid power systems, such as combinations of renewable power sources with intermittent power production and non-renewable power sources, theoretically increase the reliability and thus integration of renewable sources in the electrical system. However, a recent increase in the number of hybrid installations has sparked interest in the effects of their connection to the grid, especially in remote areas. This paper analyses a photovoltaic-gas microturbine hybrid system dimensioned to be installed in La Paz (Mexico.The research presented in this paper studies and quantifies the effects on the total electric power produced, varying both the solar radiation and the gas microturbine response time. The gas microturbine and the photovoltaic panels are modelled using Matlab/Simulink software, obtaining a platform where different tests to simulate real conditions have been executed. They consist of diverse ramps of irradiance that replicate solar radiation variations, and different microturbine response times reproduced by the time constants of a first order transfer function that models the microturbine dynamic response. The results obtained show that when radiation varies quickly it does not produce significant differences in the power guarantee or the microturbine gas consumption, to any microturbine response time. However, these two parameters are highly variable with smooth radiance variations. The maximum total power variation decreases greatly as the radiation variation gets lower. In addition, by decreasing the microturbine response time, it is possible to appreciably increase the power guarantee although the maximum power variation and gas consumption increase. Only in cases of low radiation variation is there no appreciable difference in the maximum power variation obtained by the different turbine response times.

  7. Simulations of hybrid system varying solar radiation and microturbine response time

    Energy Technology Data Exchange (ETDEWEB)

    Fernández Ribaya, Yolanda, E-mail: fernandezryolanda@uniovi.es; Álvarez, Eduardo; Paredes Sánchez, José Pablo; Xiberta Bernat, Jorge [Department of Energy E.I.M.E.M., University of Oviedo. 13 Independencia Street 2" n" d floor, 36004, Oviedo (Spain)

    2015-07-15

    Hybrid power systems, such as combinations of renewable power sources with intermittent power production and non-renewable power sources, theoretically increase the reliability and thus integration of renewable sources in the electrical system. However, a recent increase in the number of hybrid installations has sparked interest in the effects of their connection to the grid, especially in remote areas. This paper analyses a photovoltaic-gas microturbine hybrid system dimensioned to be installed in La Paz (Mexico).The research presented in this paper studies and quantifies the effects on the total electric power produced, varying both the solar radiation and the gas microturbine response time. The gas microturbine and the photovoltaic panels are modelled using Matlab/Simulink software, obtaining a platform where different tests to simulate real conditions have been executed. They consist of diverse ramps of irradiance that replicate solar radiation variations, and different microturbine response times reproduced by the time constants of a first order transfer function that models the microturbine dynamic response. The results obtained show that when radiation varies quickly it does not produce significant differences in the power guarantee or the microturbine gas consumption, to any microturbine response time. However, these two parameters are highly variable with smooth radiance variations. The maximum total power variation decreases greatly as the radiation variation gets lower. In addition, by decreasing the microturbine response time, it is possible to appreciably increase the power guarantee although the maximum power variation and gas consumption increase. Only in cases of low radiation variation is there no appreciable difference in the maximum power variation obtained by the different turbine response times.

  8. Evaluation of the agronomic performance of atrazine-tolerant transgenic japonica rice parental lines for utilization in hybrid seed production.

    Directory of Open Access Journals (Sweden)

    Luhua Zhang

    Full Text Available Currently, the purity of hybrid seed is a crucial limiting factor when developing hybrid japonica rice (Oryza sativa L.. To chemically control hybrid seed purity, we transferred an improved atrazine chlorohydrolase gene (atzA from Pseudomonas ADP into hybrid japonica parental lines (two maintainers, one restorer, and Nipponbare, by using Agrobacterium-mediated transformation. We subsequently selected several transgenic lines from each genotype by using PCR, RT-PCR, and germination analysis. In the presence of the investigated atrazine concentrations, particularly 150 µM atrazine, almost all of the transgenic lines produced significantly larger seedlings, with similar or higher germination percentages, than did the respective controls. Although the seedlings of transgenic lines were taller and gained more root biomass compared to the respective control plants, their growth was nevertheless inhibited by atrazine treatment compared to that without treatment. When grown in soil containing 2 mg/kg or 5 mg/kg atrazine, the transgenic lines were taller, and had higher total chlorophyll contents than did the respective controls; moreover, three of the strongest transgenic lines completely recovered after 45 days of growth. After treatment with 2 mg/kg or 5 mg/kg of atrazine, the atrazine residue remaining in the soil was 2.9-7.0% or 0.8-8.7% respectively, for transgenic lines, and 44.0-59.2% or 28.1-30.8%, respectively, for control plants. Spraying plants at the vegetative growth stage with 0.15% atrazine effectively killed control plants, but not transgenic lines. Our results indicate that transgenic atzA rice plants show tolerance to atrazine, and may be used as parental lines in future hybrid seed production.

  9. Tissue culture-induced genetic and epigenetic alterations in rice pure-lines, F1 hybrids and polyploids.

    Science.gov (United States)

    Wang, Xiaoran; Wu, Rui; Lin, Xiuyun; Bai, Yan; Song, Congdi; Yu, Xiaoming; Xu, Chunming; Zhao, Na; Dong, Yuzhu; Liu, Bao

    2013-05-05

    Genetic and epigenetic alterations can be invoked by plant tissue culture, which may result in heritable changes in phenotypes, a phenomenon collectively termed somaclonal variation. Although extensive studies have been conducted on the molecular nature and spectrum of tissue culture-induced genomic alterations, the issue of whether and to what extent distinct plant genotypes, e.g., pure-lines, hybrids and polyploids, may respond differentially to the tissue culture condition remains poorly understood. We investigated tissue culture-induced genetic and epigenetic alterations in a set of rice genotypes including two pure-lines (different subspecies), a pair of reciprocal F1 hybrids parented by the two pure-lines, and a pair of reciprocal tetraploids resulted from the hybrids. Using two molecular markers, amplified fragment length polymorphism (AFLP) and methylation-sensitive amplified polymorphism (MSAP), both genetic and DNA methylation alterations were detected in calli and regenerants from all six genotypes, but genetic alteration is more prominent than epigenetic alteration. While significant genotypic difference was observed in frequencies of both types of alterations, only genetic alteration showed distinctive features among the three types of genomes, with one hybrid (N/9) being exceptionally labile. Surprisingly, difference in genetic alteration frequencies between the pair of reciprocal F1 hybrids is much greater than that between the two pure-line subspecies. Difference also exists in the pair of reciprocal tetraploids, but is to a less extent than that between the hybrids. The steady-state transcript abundance of genes involved in DNA repair and DNA methylation was significantly altered in both calli and regenerants, and some of which were correlated with the genetic and/or epigenetic alterations. Our results, based on molecular marker analysis of ca. 1,000 genomic loci, document that genetic alteration is the major cause of somaclonal variation in rice

  10. Intercomparison of three microwave/infrared high resolution line-by-line radiative transfer codes

    Science.gov (United States)

    Schreier, Franz; Milz, Mathias; Buehler, Stefan A.; von Clarmann, Thomas

    2018-05-01

    An intercomparison of three line-by-line (lbl) codes developed independently for atmospheric radiative transfer and remote sensing - ARTS, GARLIC, and KOPRA - has been performed for a thermal infrared nadir sounding application assuming a HIRS-like (High resolution Infrared Radiation Sounder) setup. Radiances for the 19 HIRS infrared channels and a set of 42 atmospheric profiles from the "Garand dataset" have been computed. The mutual differences of the equivalent brightness temperatures are presented and possible causes of disagreement are discussed. In particular, the impact of path integration schemes and atmospheric layer discretization is assessed. When the continuum absorption contribution is ignored because of the different implementations, residuals are generally in the sub-Kelvin range and smaller than 0.1 K for some window channels (and all atmospheric models and lbl codes). None of the three codes turned out to be perfect for all channels and atmospheres. Remaining discrepancies are attributed to different lbl optimization techniques. Lbl codes seem to have reached a maturity in the implementation of radiative transfer that the choice of the underlying physical models (line shape models, continua etc) becomes increasingly relevant.

  11. Maize forage aptitude: Combining ability of inbred lines and stability of hybrids

    Directory of Open Access Journals (Sweden)

    Luis Máximo Bertoia

    2014-12-01

    Full Text Available Breeding of forage maize should combine improvement achieved for grain with the specific needs of forage hybrids. Production stability is important when maize is used for silage if the planting area is not in the ideal agronomic environment. The objectives of the present research were: (i to quantify environmental and genetic and their interaction effects on maize silage traits; (ii to identify possible heterotic groups for forage aptitude and suggest the formation of potential heterotic patterns, and (iii to identify suitable inbred line combinations for producing hybrids with forage aptitude. Forty-five hybrids derived from diallelic crosses (without reciprocals among ten inbred lines of maize were evaluated in this study. Combined ANOVA over environments showed differences between genotypes (G, environments (E, and their interactions (GEI. Heritability (H2, and genotypic and phenotypic correlations were estimated to evaluate the variation in and relationships between forage traits. Postdictive and predictive AMMI models were fitted to determine the importance of each source of variation, G, E, and GEI, and to select genotypes simultaneously on yield, quality and stability. A predominance of additive effects was found in the evaluated traits. The heterotic pattern Reid-BSSS × Argentine flint was confirmed for ear yield (EY and harvest index (HI. High and broad genetic variation was found for stover and whole plant traits. Some inbred lines had genes with differential breeding aptitude for ear and stover. Stover and ear yield should be the main breeding objectives in maize forage breeding.

  12. Design and development of radiation absorber for sighting beam line

    International Nuclear Information System (INIS)

    Sridhar, R.; Shukla, S.K.

    2005-01-01

    During the commissioning of Indus-2 , it is necessary to view the synchrotron radiation that will be emanating from the dipole exit ports. The 10 0 beam line from dipole 11 was earmarked for sighting beam line. The synchrotron radiation power density would be around 340 watts on the photon absorber inside the radiation absorber module, at the specified beam power of Indus-2. The beam striking on this photon absorber produces x-rays and Bremsstrahlung radiation. These are to be stopped and absorbed by radiation absorber. The photon absorber and the radiation absorber are integrated in a single vacuum chamber and actuated by a pneumatic cylinder connected using a bellow. Radiation absorber was needed to isolate the diagnostic components and to protect them from radiation a well as heat when they were not in use. The paper describes the design, calculation and development of the dynamic photon cum radiation absorber. The ultimate vacuum performance is also described. (author)

  13. Investigation of Magnetospheric Line Radiation above China

    Science.gov (United States)

    Sheng, X.; Wu, J.; Pu, X.

    2017-12-01

    Magnetospheric Line Radiation (MLR) is a kind of VLF emission that is considered by some researchers to be related with the power system on ground, and in frequency-time spectrograms of electromagnetic field, it has a line structure with large frequency bandwidth. These emission waves propagate through the magnetosphere and strongly interact with energetic electrons trapped in the earth's magnetic field. Such a wave-particle interaction amplifies the radiation and scatters energetic electrons, which may trigger new radiations. We detected 328 MLR events by analyzing the electric field data observed by DEMETER satellite in the space above China from the year of 2008 to 2010. Their characteristics and possible cause have been investigated systematically. There were more MLR events in daytime than in nighttime and more in winter than in summer. Such diurnal and seasonal differences were closely associated with whistlers and ionosphere conditions. Comparing Kp indices at the occurring time of MLR events and nationwide Kp indices through the analyzed years, we found these MLR events were not significantly dependent on geomagnetic activity. Most of events were distributed in the low latitude, while their peak intensities in frequency-time spectrograms seemed to be independent of latitude. The frequency intervals of MLR events were between 50 to 95Hz, and the frequency drifts were mostly in 0 0.4Hz/s. The above characteristics of MLR events were similar to those of Power Line Harmonic Radiation (PLHR) events observed in the space above China, therefore we inferred that these two emissions have close relation.

  14. Hybrid mimics and hybrid vigor in Arabidopsis

    Science.gov (United States)

    Wang, Li; Greaves, Ian K.; Groszmann, Michael; Wu, Li Min; Dennis, Elizabeth S.; Peacock, W. James

    2015-01-01

    F1 hybrids can outperform their parents in yield and vegetative biomass, features of hybrid vigor that form the basis of the hybrid seed industry. The yield advantage of the F1 is lost in the F2 and subsequent generations. In Arabidopsis, from F2 plants that have a F1-like phenotype, we have by recurrent selection produced pure breeding F5/F6 lines, hybrid mimics, in which the characteristics of the F1 hybrid are stabilized. These hybrid mimic lines, like the F1 hybrid, have larger leaves than the parent plant, and the leaves have increased photosynthetic cell numbers, and in some lines, increased size of cells, suggesting an increased supply of photosynthate. A comparison of the differentially expressed genes in the F1 hybrid with those of eight hybrid mimic lines identified metabolic pathways altered in both; these pathways include down-regulation of defense response pathways and altered abiotic response pathways. F6 hybrid mimic lines are mostly homozygous at each locus in the genome and yet retain the large F1-like phenotype. Many alleles in the F6 plants, when they are homozygous, have expression levels different to the level in the parent. We consider this altered expression to be a consequence of transregulation of genes from one parent by genes from the other parent. Transregulation could also arise from epigenetic modifications in the F1. The pure breeding hybrid mimics have been valuable in probing the mechanisms of hybrid vigor and may also prove to be useful hybrid vigor equivalents in agriculture. PMID:26283378

  15. Thoughts and Practice on Some Problems about Research and Application of Two-Line Hybrid Rice

    Directory of Open Access Journals (Sweden)

    Li-yun CHEN

    2011-06-01

    Full Text Available The main problems about research and application of two-line hybrid rice were reviewed, including the confusing nomenclature and male sterile lines classification, the unclear characteristics of photoperiod and temperature responses and the unsuitable site selection for male sterile line and hybrid rice seed production. In order to efficiently and accurately use dual-purpose genic male sterile lines, four types, including PTGMS (photo-thermo-sensitive genic male sterile rice, TGMS (thermo-sensitive genic male sterile rice, reverse PTGMS and reverse TGMS, were proposed. A new idea for explaining the mechanism of sterility in dual-purpose hybrid rice was proposed. The transition from sterile to fertile was involved in the cooperative regulation of major-effect sterile genes and photoperiod and/or temperature sensitive ones. The minor-effect genes with accumulative effect on sterility were important factors that affected the critical temperature of sterility transfer. In order to make better use of dual-purpose lines, the characterization of responses to photoperiod and temperature of PTGMS should be made and the identification method for the characterization of photoperiod and temperature responses of PTGMS should also be put forward. The optimal ecological site for seed production could be determined according to the historical climate data and the requirements for the meteorological conditions during the different periods of seed production.

  16. Forecasting solar radiation using an optimized hybrid model by Cuckoo Search algorithm

    International Nuclear Information System (INIS)

    Wang, Jianzhou; Jiang, He; Wu, Yujie; Dong, Yao

    2015-01-01

    Due to energy crisis and environmental problems, it is very urgent to find alternative energy sources nowadays. Solar energy, as one of the great potential clean energies, has widely attracted the attention of researchers. In this paper, an optimized hybrid method by CS (Cuckoo Search) on the basis of the OP-ELM (Optimally Pruned Extreme Learning Machine), called CS-OP-ELM, is developed to forecast clear sky and real sky global horizontal radiation. First, MRSR (Multiresponse Sparse Regression) and LOO-CV (leave-one-out cross-validation) can be applied to rank neurons and prune the possibly meaningless neurons of the FFNN (Feed Forward Neural Network), respectively. Then, Direct strategy and Direct-Recursive strategy based on OP-ELM are introduced to build a hybrid model. Furthermore, CS (Cuckoo Search) optimized algorithm is employed to determine the proper weight coefficients. In order to verify the effectiveness of the developed method, hourly solar radiation data from six sites of the United States has been collected, and methods like ARMA (Autoregression moving average), BP (Back Propagation) neural network and OP-ELM can be compared with CS-OP-ELM. Experimental results show the optimized hybrid method CS-OP-ELM has the best forecasting performance. - Highlights: • An optimized hybrid method called CS-OP-ELM is proposed to forecast solar radiation. • CS-OP-ELM adopts multiple variables dataset as input variables. • Direct and Direct-Recursive strategy are introduced to build a hybrid model. • CS (Cuckoo Search) algorithm is used to determine the optimal weight coefficients. • The proposed method has the best performance compared with other methods

  17. Chemosensitivity and radiosensitivity of small cell lung cancer cell lines studied by a newly developed 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) hybrid assay

    International Nuclear Information System (INIS)

    Hida, T.; Ueda, R.; Takahashi, T.; Watanabe, H.; Kato, T.; Suyama, M.; Sugiura, T.; Ariyoshi, Y.

    1989-01-01

    The 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) hybrid assay was developed by technically combining the human tumor clonogenic assay and the MTT assay to make the most of both assays. This assay was able to estimate the in vitro growth of cultured cell lines and of tumor cells in pleural effusion, suggesting the possibility of its use for assessment of chemosensitivity and radiosensitivity of fresh tumor samples. Multiple cell lines [including morphological and/or phenotypical in vitro converters and cisplatin (CDDP)-resistant lines] were established from three patients with small cell lung cancer at different stages of the disease. Chemosensitivity of these cell lines to four commonly used chemotherapeutic drugs was tested by the MTT hybrid assay. SK1 and SK3 lines were established from Patient S. K. before and after chemotherapy and radiotherapy, respectively. SK3/CDDP, a CDDP-resistant line derived from the SK3 line, was 30-fold more resistant to CDDP [50% inhibiting dose (IC50), 21.5 micrograms/ml] than the SK1 line. In Patient M. O., MOA2/CDDP, a CDDP-resistant line derived from MOA2 (an in vitro converter from the MO line), was 41-fold more resistant to CDDP (IC50, 37 micrograms/ml) than the parent MO line. From Patient T. M., TM1 and TM2 lines were established before and after chemotherapy, respectively. The latter showed 6-fold more resistance to CDDP than the former. Chemosensitivity of these lines to three other drugs, 4-hydroperoxycyclophosphamide, Adriamycin, and etoposide, suggested cross-resistance between CDDP and 4-hydroperoxycyclophosphamide. Radiosensitivity study was also carried out with the MTT hybrid assay. The MOA2 line was more resistant [Do, 3.0 Gy; extrapolation number (n), 4.0] than the parental MO line (Do, 1.6 Gy; n, 2.1). There was no clear difference in radiosensitivity between the cell lines established before and after radiation therapy in Patient S. K

  18. Application of dissociation curve analysis to radiation hybrid panel marker scoring: generation of a map of river buffalo (B. bubalis chromosome 20

    Directory of Open Access Journals (Sweden)

    Schäffer Alejandro A

    2008-11-01

    Full Text Available Abstract Background Fluorescence of dyes bound to double-stranded PCR products has been utilized extensively in various real-time quantitative PCR applications, including post-amplification dissociation curve analysis, or differentiation of amplicon length or sequence composition. Despite the current era of whole-genome sequencing, mapping tools such as radiation hybrid DNA panels remain useful aids for sequence assembly, focused resequencing efforts, and for building physical maps of species that have not yet been sequenced. For placement of specific, individual genes or markers on a map, low-throughput methods remain commonplace. Typically, PCR amplification of DNA from each panel cell line is followed by gel electrophoresis and scoring of each clone for the presence or absence of PCR product. To improve sensitivity and efficiency of radiation hybrid panel analysis in comparison to gel-based methods, we adapted fluorescence-based real-time PCR and dissociation curve analysis for use as a novel scoring method. Results As proof of principle for this dissociation curve method, we generated new maps of river buffalo (Bubalus bubalis chromosome 20 by both dissociation curve analysis and conventional marker scoring. We also obtained sequence data to augment dissociation curve results. Few genes have been previously mapped to buffalo chromosome 20, and sequence detail is limited, so 65 markers were screened from the orthologous chromosome of domestic cattle. Thirty bovine markers (46% were suitable as cross-species markers for dissociation curve analysis in the buffalo radiation hybrid panel under a standard protocol, compared to 25 markers suitable for conventional typing. Computational analysis placed 27 markers on a chromosome map generated by the new method, while the gel-based approach produced only 20 mapped markers. Among 19 markers common to both maps, the marker order on the map was maintained perfectly. Conclusion Dissociation curve

  19. Radiation response of mouse lymphoid and myeloid cell lines. Pt. 3

    International Nuclear Information System (INIS)

    Radford, I.R.; Murphy, T.K.

    1994-01-01

    The authors have examined the timing of γ-irradiation-induced death in relation to cell cycle progression using a panel of mouse lymphoid or myeloid cell lines. Death was found to occur immediately after irradiation ('rapid interphase' death), or after arrest in G 2 phase ('delayed interphase' death), or following one or more mitoses ('mitotic/delayed mitotic' death). In part II of this series of papers the authors demonstrated the occurrence of radiation-induced apoptosis in all these cell lines. Several of the cell lines showed different timing of death dependent upon the radiation dose used. These differences in the timing of radiation-induced death are shown to be useful indicators of the relative radiosensitivity of haematopoietic cell lines. (author)

  20. Computational hybrid anthropometric paediatric phantom library for internal radiation dosimetry

    DEFF Research Database (Denmark)

    Xie, Tianwu; Kuster, Niels; Zaidi, Habib

    2017-01-01

    for children demonstrated that they follow the same trend when correlated with age. The constructed hybrid computational phantom library opens up the prospect of comprehensive radiation dosimetry calculations and risk assessment for the paediatric population of different age groups and diverse anthropometric...

  1. Reliable Radiation Hybrid Maps: An Efficient Scalable Clustering-based Approach

    Science.gov (United States)

    The process of mapping markers from radiation hybrid mapping (RHM) experiments is equivalent to the traveling salesman problem and, thereby, has combinatorial complexity. As an additional problem, experiments typically result in some unreliable markers that reduce the overall quality of the map. We ...

  2. Beta-cell lines derived from transgenic mice expressing a hybrid insulin gene-oncogene

    DEFF Research Database (Denmark)

    Efrat, S; Linde, S; Kofod, Hans

    1988-01-01

    Three pancreatic beta-cell lines have been established from insulinomas derived from transgenic mice carrying a hybrid insulin-promoted simian virus 40 tumor antigen gene. The beta tumor cell (beta TC) lines maintain the features of differentiated beta cells for about 50 passages in culture. The ...... both to immortalize a rare cell type and to provide a selection for the maintenance of its differentiated phenotype....

  3. Genetic characterization of Bombyx mori (Lepidoptera: Bombycidae) breeding and hybrid lines with different geographic origins.

    Science.gov (United States)

    Furdui, Emilia M; Mărghitaş, Liviu A; Dezmirean, Daniel S; Paşca, Ioan; Pop, Iulia F; Erler, Silvio; Schlüns, Ellen A

    2014-01-01

    The domesticated silkworm Bombyx mori L. comprises a large number of geographical breeds and hybrid lines. Knowing the genetic structure of those may provide information to improve the conservation of commercial lines by estimating inbreeding over generations and the consequences of excessive use of those lineages. Here, we analyzed the genetic diversity of seven breeds and eight hybrid lines from Eastern Europe and Asia using highly polymorphic microsatellites markers to determine its genetical impact on their use in global breeding programs. No consistent pattern of deviation from Hardy-Weinberg equilibrium was found for most breed and hybrids; and the absence of a linkage disequilibrium also suggests that the strains are in equilibrium. A principal coordinate analysis revealed a clear separation of two silkworm breeds from the rest: one (IBV) originated from India and the other one (RG90) from Romania/Japan. The tendency of the other breeds from different geographic origins to cluster together in a general mix might be due to similar selection pressures (climate and anthropogenic factors) in different geographic locations. Phylogenetic analyses grouped the different silkworm breeds but not the hybrids according to their geographic origin and confirmed the pattern found in the principal coordinate analysis. © The Author 2014. Published by Oxford University Press on behalf of the Entomological Society of America.

  4. An Efficient Hybrid Conjugate Gradient Method with the Strong Wolfe-Powell Line Search

    Directory of Open Access Journals (Sweden)

    Ahmad Alhawarat

    2015-01-01

    Full Text Available Conjugate gradient (CG method is an interesting tool to solve optimization problems in many fields, such as design, economics, physics, and engineering. In this paper, we depict a new hybrid of CG method which relates to the famous Polak-Ribière-Polyak (PRP formula. It reveals a solution for the PRP case which is not globally convergent with the strong Wolfe-Powell (SWP line search. The new formula possesses the sufficient descent condition and the global convergent properties. In addition, we further explained about the cases where PRP method failed with SWP line search. Furthermore, we provide numerical computations for the new hybrid CG method which is almost better than other related PRP formulas in both the number of iterations and the CPU time under some standard test functions.

  5. A hybrid computational approach to estimate solar global radiation: An empirical evidence from Iran

    International Nuclear Information System (INIS)

    Mostafavi, Elham Sadat; Ramiyani, Sara Saeidi; Sarvar, Rahim; Moud, Hashem Izadi; Mousavi, Seyyed Mohammad

    2013-01-01

    This paper presents an innovative hybrid approach for the estimation of the solar global radiation. New prediction equations were developed for the global radiation using an integrated search method of genetic programming (GP) and simulated annealing (SA), called GP/SA. The solar radiation was formulated in terms of several climatological and meteorological parameters. Comprehensive databases containing monthly data collected for 6 years in two cities of Iran were used to develop GP/SA-based models. Separate models were established for each city. The generalization of the models was verified using a separate testing database. A sensitivity analysis was conducted to investigate the contribution of the parameters affecting the solar radiation. The derived models make accurate predictions of the solar global radiation and notably outperform the existing models. -- Highlights: ► A hybrid approach is presented for the estimation of the solar global radiation. ► The proposed method integrates the capabilities of GP and SA. ► Several climatological and meteorological parameters are included in the analysis. ► The GP/SA models make accurate predictions of the solar global radiation.

  6. Investigation of Genetic Distance among Parental Lines of Hybrid Rice Based on Cluster Analysis of Morphological Traits

    Directory of Open Access Journals (Sweden)

    A. Baluch-Zehi

    2013-06-01

    Full Text Available conditions. Thus, these varieties could be suitable option for yield increase and an effective step toward food security. Selection of parental lines has essential role in developing ideal combinations. Therefore, it is essential to study the relationship and genetic diversity among parental lines in hybrid rice. Sixteen hybrid rice parental lines including 6 restorer lines (Poya, Sepidrud, Pajohesh, R2, R9 and IR50 and 5 CMS lines (Neda, Nemat, Dasht, Champa and Amol 3 with their 5 maintainers were studied at Research Farm of Sari Agricultural Sciences and Natural Resources University during 2011. Analysis of variance showed significant variations for all of the studied traits, which shows great diversity among the genotypes. The number of fertile tillers and length to width ratio of grain showed positive and significant correlation with yield. But, grain width showed negative and significant correlation with yield. Results of principal component analysis revealed that 3 components explained 75.64% of the total variations. Cluster analysis at 15 genetic distance criteria grouped genotypes in 4 clusters. In exploration of heterosis phenomenon, parents must be far away from each other. So, the results of this study suggested crosses between CMS lines of Neda A, Nemat A and Champa A with each of restorer lines R9, R2, IR50 and Poya for experimental hybrid seed production.

  7. Comparative proteome analysis of drought-sensitive and drought-tolerant rapeseed roots and their hybrid F1 line under drought stress.

    Science.gov (United States)

    Mohammadi, Payam Pour; Moieni, Ahmad; Komatsu, Setsuko

    2012-11-01

    Rapeseed (Brassica napus L.), which is the third leading source of vegetable oil, is sensitive to drought stress during the early vegetative growth stage. To investigate the initial response of rapeseed to drought stress, changes in the protein expression profiles of drought-sensitive (RGS-003) and drought-tolerant lines (SLM-003), and their F1 hybrid, were analyzed using a proteomics approach. Seven-day-old rapeseed seedlings were treated with drought stress by restricting water for 7 days, and proteins were extracted from roots and separated by two-dimensional polyacrylamide gel electrophoresis. In the sensitive rapeseed line, 35 protein spots were differentially expressed under drought stress, and proteins related to metabolism, energy, disease/defense, and transport were decreased. In the tolerant line, 32 protein spots were differentially expressed under drought stress, and proteins involved in metabolism, disease/defense, and transport were increased, while energy-related proteins were decreased. Six protein spots in F1 hybrid were common among expressed proteins in the drought-sensitive and -tolerant lines. Notably, tubulin beta-2 and heat shock protein 70 were decreased in the drought-sensitive line and hybrid F1 plants, while jasmonate-inducible protein and 20S proteasome subunit PAF1 were increased in the F1 hybrids and drought-tolerant line. These results indicate that (1) V-type H(+) ATPase, plasma-membrane associated cation-binding protein, HSP 90, and elongation factor EF-2 have a role in the drought tolerance of rapeseed; (2) The decreased levels of heat shock protein 70 and tubulin beta-2 in the drought-sensitive and hybrid F1 lines might explain the reduced growth of these lines in drought conditions.

  8. Hybrid finite volume/ finite element method for radiative heat transfer in graded index media

    Science.gov (United States)

    Zhang, L.; Zhao, J. M.; Liu, L. H.; Wang, S. Y.

    2012-09-01

    The rays propagate along curved path determined by the Fermat principle in the graded index medium. The radiative transfer equation in graded index medium (GRTE) contains two specific redistribution terms (with partial derivatives to the angular coordinates) accounting for the effect of the curved ray path. In this paper, the hybrid finite volume with finite element method (hybrid FVM/FEM) (P.J. Coelho, J. Quant. Spectrosc. Radiat. Transf., vol. 93, pp. 89-101, 2005) is extended to solve the radiative heat transfer in two-dimensional absorbing-emitting-scattering graded index media, in which the spatial discretization is carried out using a FVM, while the angular discretization is by a FEM. The FEM angular discretization is demonstrated to be preferable in dealing with the redistribution terms in the GRTE. Two stiff matrix assembly schemes of the angular FEM discretization, namely, the traditional assembly approach and a new spherical assembly approach (assembly on the unit sphere of the solid angular space), are discussed. The spherical assembly scheme is demonstrated to give better results than the traditional assembly approach. The predicted heat flux distributions and temperature distributions in radiative equilibrium are determined by the proposed method and compared with the results available in other references. The proposed hybrid FVM/FEM method can predict the radiative heat transfer in absorbing-emitting-scattering graded index medium with good accuracy.

  9. Hybrid finite volume/ finite element method for radiative heat transfer in graded index media

    International Nuclear Information System (INIS)

    Zhang, L.; Zhao, J.M.; Liu, L.H.; Wang, S.Y.

    2012-01-01

    The rays propagate along curved path determined by the Fermat principle in the graded index medium. The radiative transfer equation in graded index medium (GRTE) contains two specific redistribution terms (with partial derivatives to the angular coordinates) accounting for the effect of the curved ray path. In this paper, the hybrid finite volume with finite element method (hybrid FVM/FEM) (P.J. Coelho, J. Quant. Spectrosc. Radiat. Transf., vol. 93, pp. 89-101, 2005) is extended to solve the radiative heat transfer in two-dimensional absorbing-emitting-scattering graded index media, in which the spatial discretization is carried out using a FVM, while the angular discretization is by a FEM. The FEM angular discretization is demonstrated to be preferable in dealing with the redistribution terms in the GRTE. Two stiff matrix assembly schemes of the angular FEM discretization, namely, the traditional assembly approach and a new spherical assembly approach (assembly on the unit sphere of the solid angular space), are discussed. The spherical assembly scheme is demonstrated to give better results than the traditional assembly approach. The predicted heat flux distributions and temperature distributions in radiative equilibrium are determined by the proposed method and compared with the results available in other references. The proposed hybrid FVM/FEM method can predict the radiative heat transfer in absorbing-emitting-scattering graded index medium with good accuracy.

  10. RF design and tests on a broadband, high-power coaxial quadrature hybrid applicable to ITER ICRF transmission line system for load-resilient operations

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Hae Jin, E-mail: haejin@nfri.re.kr [National Fusion Research Institute, Daejeon (Korea, Republic of); Wang, Son Jong; Park, Byoung Ho; Kwak, Jong-Gu [National Fusion Research Institute, Daejeon (Korea, Republic of); Hillairet, Julien [CEA/IRFM, Saint-lez-Durance (France); Choi, Jin Joo [Kwangwoon University, Seoul (Korea, Republic of)

    2015-10-15

    Highlights: • Amplitude balanced 3 dB coaxial hybrid splitter has been designed and rf tested. • The proposed hybrid is applicable to ITER ICRF transmission line for load resilience. • Two-section, broadband coaxial hybrid can be tunable by changing dielectric insulator. - Abstract: RF design and network analyzer tests of broadband, amplitude-balanced coaxial hybrid junctions are presented. We have designed two 3 dB hybrid splitters with 9 and 12 in. coaxial transmission lines applicable to ITER ICRF for load-resilient operations using ANSYS HFSS. Amplitude-balanced broadband responses were obtained with the combination of impedance reductions of longitudinal and transverse branches in unequal proportion, length change of 50 Ω lines and diameter change of high impedance lines connected transversely to the T-section of the hybrid splitter, respectively. We have fabricated and RF tested the 9 in. coaxial hybrid coupler. We obtained an excellent coupling flatness of −3.2 ± 0.2 dB, phase difference of 4 degrees and return loss of 16 dB in 40–55 MHz. The measured data of 9 in. hybrid splitter is highly consistent with HFSS simulations. We found that the proposed 3 dB hybrid splitter can be tunable with amplitude-balanced, broadband response by changing dielectric insulators to keep the inner and outer conductors of coaxial line apart. The proposed 3 dB hybrid splitter can be utilized for load-resilient operations in a wide range of antenna load variations due to mode transitions or edge localized modes (ELMs) in fusion plasmas.

  11. RF design and tests on a broadband, high-power coaxial quadrature hybrid applicable to ITER ICRF transmission line system for load-resilient operations

    International Nuclear Information System (INIS)

    Kim, Hae Jin; Wang, Son Jong; Park, Byoung Ho; Kwak, Jong-Gu; Hillairet, Julien; Choi, Jin Joo

    2015-01-01

    Highlights: • Amplitude balanced 3 dB coaxial hybrid splitter has been designed and rf tested. • The proposed hybrid is applicable to ITER ICRF transmission line for load resilience. • Two-section, broadband coaxial hybrid can be tunable by changing dielectric insulator. - Abstract: RF design and network analyzer tests of broadband, amplitude-balanced coaxial hybrid junctions are presented. We have designed two 3 dB hybrid splitters with 9 and 12 in. coaxial transmission lines applicable to ITER ICRF for load-resilient operations using ANSYS HFSS. Amplitude-balanced broadband responses were obtained with the combination of impedance reductions of longitudinal and transverse branches in unequal proportion, length change of 50 Ω lines and diameter change of high impedance lines connected transversely to the T-section of the hybrid splitter, respectively. We have fabricated and RF tested the 9 in. coaxial hybrid coupler. We obtained an excellent coupling flatness of −3.2 ± 0.2 dB, phase difference of 4 degrees and return loss of 16 dB in 40–55 MHz. The measured data of 9 in. hybrid splitter is highly consistent with HFSS simulations. We found that the proposed 3 dB hybrid splitter can be tunable with amplitude-balanced, broadband response by changing dielectric insulators to keep the inner and outer conductors of coaxial line apart. The proposed 3 dB hybrid splitter can be utilized for load-resilient operations in a wide range of antenna load variations due to mode transitions or edge localized modes (ELMs) in fusion plasmas.

  12. Enhancement of Radiation Response in Osteosarcoma and Rhabomyosarcoma Cell Lines by Histone Deacetylase Inhibition

    International Nuclear Information System (INIS)

    Blattmann, Claudia; Oertel, Susanne; Ehemann, Volker

    2010-01-01

    Purpose: Histone deacetylase inhibitors (HDACIs) can enhance the sensitivity of cells to photon radiation treatment (XRT) by altering numerous molecular pathways. We investigated the effect of pan-HDACIs such as suberoylanilide hydroxamic acid (SAHA) on radiation response in two osteosarcoma (OS) and two rhabdomyosarcoma (RMS) cell lines. Methods and Materials: Clonogenic survival, cell cycle analysis, and apoptosis were examined in OS (KHOS-24OS, SAOS2) and RMS (A-204, RD) cell lines treated with HDACI and HDACI plus XRT, respectively. Protein expression was investigated via immunoblot analysis, and cell cycle analysis and measurement of apoptosis were performed using flow cytometry. Results: SAHA induced an inhibition of cell proliferation and clonogenic survival in OS and RMS cell lines and led to a significant radiosensitization of all tumor cell lines. Other HDACI such as M344 and valproate showed similar effects as investigated in one OS cell line. Furthermore, SAHA significantly increased radiation-induced apoptosis in the OS cell lines, whereas in the RMS cell lines radiation-induced apoptosis was insignificant with and without SAHA. In all investigated sarcoma cell lines, SAHA attenuated radiation-induced DNA repair protein expression (Rad51, Ku80). Conclusion: Our results show that HDACIs enhance radiation action in OS and RMS cell lines. Inhibition of DNA repair, as well as increased apoptosis induction after exposure to HDACIs, can be mechanisms of radiosensitization by HDACIs.

  13. Computer Modeling of Radiative Transfer in Hybrid-Stabilized Argon–Water Electric Arc

    Czech Academy of Sciences Publication Activity Database

    Jeništa, Jiří; Takana, H.; Nishiyama, H.; Křenek, Petr; Bartlová, M.; Aubrecht, V.

    2011-01-01

    Roč. 39, č. 11 (2011), s. 2892-2893 ISSN 0093-3813 Institutional research plan: CEZ:AV0Z20430508 Keywords : Divergence of radiation flux * hybrid-stabilized electric arc * mass flow rate * partial characteristics * radiation flux Subject RIV: BL - Plasma and Gas Discharge Physics Impact factor: 1.174, year: 2011 http://ieeexplore.ieee.org/xpl/RecentIssue.jsp?punumber=27

  14. Radiation Resistant Hybrid Lotus Effect Photoelectrocatalytic Self-Cleaning Anti-Contamination Coatings, Phase I

    Data.gov (United States)

    National Aeronautics and Space Administration — This project will develop radiation resistant hybrid Lotus Effect photoelectrocatalytic self-cleaning anti-contamination coatings for application to Lunar...

  15. Dissecting plant chromosomes by the use of ionizing radiation

    Science.gov (United States)

    Radiation treatment of genomes is used to generate chromosome breaks for numerous applications. This protocol describes the preparation of seeds and the determination of the optimal level of irradiation dosage for the creation of a radiation hybrid (RH) population. These RH lines can be used to gene...

  16. A hybrid Boundary Element Unstructured Transmission-line (BEUT) method for accurate 2D electromagnetic simulation

    Energy Technology Data Exchange (ETDEWEB)

    Simmons, Daniel, E-mail: daniel.simmons@nottingham.ac.uk; Cools, Kristof; Sewell, Phillip

    2016-11-01

    Time domain electromagnetic simulation tools have the ability to model transient, wide-band applications, and non-linear problems. The Boundary Element Method (BEM) and the Transmission Line Modeling (TLM) method are both well established numerical techniques for simulating time-varying electromagnetic fields. The former surface based method can accurately describe outwardly radiating fields from piecewise uniform objects and efficiently deals with large domains filled with homogeneous media. The latter volume based method can describe inhomogeneous and non-linear media and has been proven to be unconditionally stable. Furthermore, the Unstructured TLM (UTLM) enables modelling of geometrically complex objects by using triangular meshes which removes staircasing and unnecessary extensions of the simulation domain. The hybridization of BEM and UTLM which is described in this paper is named the Boundary Element Unstructured Transmission-line (BEUT) method. It incorporates the advantages of both methods. The theory and derivation of the 2D BEUT method is described in this paper, along with any relevant implementation details. The method is corroborated by studying its correctness and efficiency compared to the traditional UTLM method when applied to complex problems such as the transmission through a system of Luneburg lenses and the modelling of antenna radomes for use in wireless communications. - Graphical abstract:.

  17. Densely ionizing radiation affects DNA methylation of selective LINE-1 elements

    Energy Technology Data Exchange (ETDEWEB)

    Prior, Sara; Miousse, Isabelle R. [Department of Environmental and Occupational Health, Fay W. Boozman College of Public Health, University of Arkansas for Medical Sciences, Little Rock, AR 72205 (United States); Nzabarushimana, Etienne [Department of Environmental and Occupational Health, Fay W. Boozman College of Public Health, University of Arkansas for Medical Sciences, Little Rock, AR 72205 (United States); Department of Bioinformatics, School of Informatics and Computing, Indiana University, Bloomington, IN 47405 (United States); Pathak, Rupak [Division of Radiation Health, Department of Pharmaceutical Sciences, College of Pharmacy, University of Arkansas for Medical Sciences, Little Rock, AR 72205 (United States); Skinner, Charles; Kutanzi, Kristy R. [Department of Environmental and Occupational Health, Fay W. Boozman College of Public Health, University of Arkansas for Medical Sciences, Little Rock, AR 72205 (United States); Allen, Antiño R. [Division of Radiation Health, Department of Pharmaceutical Sciences, College of Pharmacy, University of Arkansas for Medical Sciences, Little Rock, AR 72205 (United States); Raber, Jacob [Departments of Behavioral Neuroscience, Neurology, and Radiation Medicine, Division of Neuroscience, ONPRC, Oregon Health & Science University, Portland, OR 97239 (United States); Tackett, Alan J. [Department of Biochemistry, College of Medicine, University of Arkansas for Medical Sciences, Little Rock, AR 72205 (United States); Hauer-Jensen, Martin [Division of Radiation Health, Department of Pharmaceutical Sciences, College of Pharmacy, University of Arkansas for Medical Sciences, Little Rock, AR 72205 (United States); Nelson, Gregory A. [Department of Basic Sciences, Division of Radiation Research, Loma Linda University, Loma Linda, CA 92350 (United States); and others

    2016-10-15

    Long Interspersed Nucleotide Element 1 (LINE-1) retrotransposons are heavily methylated and are the most abundant transposable elements in mammalian genomes. Here, we investigated the differential DNA methylation within the LINE-1 under normal conditions and in response to environmentally relevant doses of sparsely and densely ionizing radiation. We demonstrate that DNA methylation of LINE-1 elements in the lungs of C57BL6 mice is dependent on their evolutionary age, where the elder age of the element is associated with the lower extent of DNA methylation. Exposure to 5-aza-2′-deoxycytidine and methionine-deficient diet affected DNA methylation of selective LINE-1 elements in an age- and promoter type-dependent manner. Exposure to densely IR, but not sparsely IR, resulted in DNA hypermethylation of older LINE-1 elements, while the DNA methylation of evolutionary younger elements remained mostly unchanged. We also demonstrate that exposure to densely IR increased mRNA and protein levels of LINE-1 via the loss of the histone H3K9 dimethylation and an increase in the H3K4 trimethylation at the LINE-1 5′-untranslated region, independently of DNA methylation. Our findings suggest that DNA methylation is important for regulation of LINE-1 expression under normal conditions, but histone modifications may dictate the transcriptional activity of LINE-1 in response to exposure to densely IR. - Highlights: • DNA methylation of LINE-1 elements is dependent on their evolutionary age. • Densely ionizing radiation affects DNA methylation of selective LINE-1 elements. • Radiation-induced reactivation of LINE-1 is DNA methylation-independent. • Histone modifications dictate the transcriptional activity of LINE-1.

  18. Densely ionizing radiation affects DNA methylation of selective LINE-1 elements

    International Nuclear Information System (INIS)

    Prior, Sara; Miousse, Isabelle R.; Nzabarushimana, Etienne; Pathak, Rupak; Skinner, Charles; Kutanzi, Kristy R.; Allen, Antiño R.; Raber, Jacob; Tackett, Alan J.; Hauer-Jensen, Martin; Nelson, Gregory A.

    2016-01-01

    Long Interspersed Nucleotide Element 1 (LINE-1) retrotransposons are heavily methylated and are the most abundant transposable elements in mammalian genomes. Here, we investigated the differential DNA methylation within the LINE-1 under normal conditions and in response to environmentally relevant doses of sparsely and densely ionizing radiation. We demonstrate that DNA methylation of LINE-1 elements in the lungs of C57BL6 mice is dependent on their evolutionary age, where the elder age of the element is associated with the lower extent of DNA methylation. Exposure to 5-aza-2′-deoxycytidine and methionine-deficient diet affected DNA methylation of selective LINE-1 elements in an age- and promoter type-dependent manner. Exposure to densely IR, but not sparsely IR, resulted in DNA hypermethylation of older LINE-1 elements, while the DNA methylation of evolutionary younger elements remained mostly unchanged. We also demonstrate that exposure to densely IR increased mRNA and protein levels of LINE-1 via the loss of the histone H3K9 dimethylation and an increase in the H3K4 trimethylation at the LINE-1 5′-untranslated region, independently of DNA methylation. Our findings suggest that DNA methylation is important for regulation of LINE-1 expression under normal conditions, but histone modifications may dictate the transcriptional activity of LINE-1 in response to exposure to densely IR. - Highlights: • DNA methylation of LINE-1 elements is dependent on their evolutionary age. • Densely ionizing radiation affects DNA methylation of selective LINE-1 elements. • Radiation-induced reactivation of LINE-1 is DNA methylation-independent. • Histone modifications dictate the transcriptional activity of LINE-1.

  19. Improvement in operational characteristics of KEPCO’s line-commutation-type superconducting hybrid fault current limiter

    International Nuclear Information System (INIS)

    Yim, S.-W.; Park, B.-C.; Jeong, Y.-T.; Kim, Y.-J.; Yang, S.-E.; Kim, W.-S.; Kim, H.-R.; Du, H.-I.

    2013-01-01

    Highlights: ► A line-commutation type hybrid FCL was modified for 1st peak current limitation. ► A superconducting module of current limitation and fault detection was fabricated. ► The superconducting module was applied to a hybrid FCL system and tested. ► 7.4 kA p fault current was limited to 4.3 kA p at the first-half cycle by the FCL. -- Abstract: A 22.9 kV class hybrid fault current limiter (FCL) developed by Korea Electric Power Corporation and LS Industrial Systems in 2006 operates using the line commutation mechanism and begins to limit the fault current after the first half-cycle. The first peak of the fault current is available for protective coordination in the power system. However, it also produces a large electromagnetic force and imposes a huge stress on power facilities such as the main transformer and gas-insulated switchgear. In this study, we improved the operational characteristics of the hybrid FCL in order to reduce the first peak of the fault current. While maintaining the structure of the hybrid FCL system, we developed a superconducting module that detects and limits the fault current during the first half-cycle. To maintain the protective coordination capacity, the hybrid FCL was designed to reduce the first peak value of the fault current by up to approximately 30%. The superconducting module was also designed to produce a minimum AC loss, generating a small, uniform magnetic field distribution during normal operation. Performance tests confirmed that when applied to the hybrid FCL, the superconducting module showed successful current limiting operation without any damage

  20. A study on application of the combination of hybridization with γ-radiation in wheat breeding

    International Nuclear Information System (INIS)

    Wang Jinxiang

    1989-11-01

    F 0 and F 1 dry seeds of winter wheat were irradiated by 60 Co γ-rays. The biological effects of M 1 , variation frequency and useful types of M 2 were investigated. Percentages of the selectivities of M 2 , M 3 and M 4 were also evaluated. The results showed that the seeds treated by combining hybridization with γ-radiation could increase variation frequency by 6∼44%, useful types by 13∼34%, and percentages of the selectivities by 6∼70%, as compared with the seeds treated only by the hybridization. Thus, the strains with high yield and protein were selected. It indicates that to combine the hybridization with γ-radiation is one of the ways for raising efficiency of wheat breeding

  1. Radiation hybrids from human chromosome 3: A basis for the construction of region and specific sublibraries

    International Nuclear Information System (INIS)

    Atchison, L.; Cosmis, R.L.; Atchison, M.L.

    1990-01-01

    The authors are interested in identifying genes on human chromosome involved in disease processes. To date at least 20 different loci on this chromosome are implicated with various disease states. DNA libraries containing clones derived from a small chromosomal subregion implicated in a particular disease would greatly assist these studies. They have utilized the radiation hybrid (RH) technique to generate a series of somatic cell hybrids that contain small segments of human chromosome 3 as the only human genetic material. A Chinese hamster-human cell hybrid (Q314-2) containing only human chromosome 3 was used to prepare radiation hybrids. Cells were lethally X-irradiated with 6,000 rads and fused to Urd(??) Chinese hamster cells by PEG 1000 treatment. The majority of hybrids (>72%) analyzed retained portions of chromosome 3. The amount of chromosome 3 in each hybrid ranged from nearly all of the chromosome to very little. Currently these hybrids are being further characterized with single copy probes of known map location in order to isolate regions of chromosome 3 that contain specific disease locus. These reduced hybrids can then be used for the construction of region specific libraries and for the generation of new DNA probes from the specific region of interest

  2. Positron energy distributions from a hybrid positron source based on channeling radiation

    International Nuclear Information System (INIS)

    Azadegan, B.; Mahdipour, A.; Dabagov, S.B.; Wagner, W.

    2013-01-01

    A hybrid positron source which is based on the generation of channeling radiation by relativistic electrons channeled along different crystallographic planes and axes of a tungsten single crystal and subsequent conversion of radiation into e + e − -pairs in an amorphous tungsten target is described. The photon spectra of channeling radiation are calculated using the Doyle–Turner approximation for the continuum potentials and classical equations of motion for channeled particles to obtain their trajectories, velocities and accelerations. The spectral-angular distributions of channeling radiation are found applying classical electrodynamics. Finally, the conversion of radiation into e + e − -pairs and the energy distributions of positrons are simulated using the GEANT4 package

  3. An Algorithm to Compress Line-transition Data for Radiative-transfer Calculations

    Science.gov (United States)

    Cubillos, Patricio E.

    2017-11-01

    Molecular line-transition lists are an essential ingredient for radiative-transfer calculations. With recent databases now surpassing the billion-line mark, handling them has become computationally prohibitive, due to both the required processing power and memory. Here I present a temperature-dependent algorithm to separate strong from weak line transitions, reformatting the large majority of the weaker lines into a cross-section data file, and retaining the detailed line-by-line information of the fewer strong lines. For any given molecule over the 0.3-30 μm range, this algorithm reduces the number of lines to a few million, enabling faster radiative-transfer computations without a significant loss of information. The final compression rate depends on how densely populated the spectrum is. I validate this algorithm by comparing Exomol’s HCN extinction-coefficient spectra between the complete (65 million line transitions) and compressed (7.7 million) line lists. Over the 0.6-33 μm range, the average difference between extinction-coefficient values is less than 1%. A Python/C implementation of this algorithm is open-source and available at https://github.com/pcubillos/repack. So far, this code handles the Exomol and HITRAN line-transition format.

  4. Contributions of each isotope in structural material on radiation damage in a hybrid reactor

    International Nuclear Information System (INIS)

    Günay, Mehtap

    2016-01-01

    In this study, the fluids were used in the liquid first-wall, blanket and shield zones of the designed hybrid reactor system. In this study, salt-heavy metal mixtures consisting of 93–85% Li_2_0Sn_8_0 + 5% SFG-PuO_2 and 2-10% UO_2, 93–85% Li_2_0Sn_8_0 + 5% SFG-PuO_2 and 2-10% NpO_2, and 93–85% Li_2_0Sn_8_0 + 5% SFG-PuO_2 and 2-10% UCO were used as fluids. In this study, the effect on the radiation damage of spent fuel-grade (SFG)-PuO_2, UO_2, NpO_2 and UCO contents was investigated in the structural material of a designed fusion–fission hybrid reactor system. In the designed hybrid reactor system were investigated the effect on the radiation damage of the selected fluid according to each isotopes of structural material in the structural material for 30 full power years (FPYs). Three-dimensional analyses were performed using the most recent MCNPX-2.7.0 Monte Carlo radiation transport code and the ENDF/B-VII.0 nuclear data library

  5. Infrared studies of galactic nebulae. IV - Continuum and line radiation from planetary nebulae.

    Science.gov (United States)

    Gillett, F. C.; Merrill, K. M.; Stein, W. A.

    1972-01-01

    Observations are reported of the detection of IR radiation from several planetary nebulae not previously known to be radiating at these wavelengths. Broad spectral bandwidth observations indicate that ir radiation in excess of that expected from atomic processes is a common phenomenon among these objects. Investigations with narrow spectral bandwidth show that in a few cases the energy in the 10.52-micron line is a significant fraction of the total energy observed in the broad-band measurements and in other cases a relatively small fraction of the total radiation. Other observations on two sources with narrow spectral bandwidth adjacent to the 10.52-micron line indicate that at these wavelengths a true continuum of radiation exists as well as lines. The results are discussed in relation to visual and radio-wavelength data.

  6. The effect of Nitrogen on Radiation Use Efficiency and Growth indices of Maize Hybrids (Zea mays L. under Kermanshah Condition

    Directory of Open Access Journals (Sweden)

    M Ahmadi

    2018-02-01

    Full Text Available Introduction Dry matter produced by crops is a function of absorbed radiation and radiation use efficiency. Radiation use efficiency is an effective approach to quantify total dry matter accumulation. It is defined as biomass produced by plant for solar radiation absorbed during growing season. Radiation use efficiency is often calculated from the linear regression slope between total dry matter accumulation and cumulative solar radiation absorbed. It is affected by species, weather conditions, crop management, plant development stages, and the production of photosynthesis compounds. Among the factors of agronomic management, nitrogen fertilizer and crop species are the most important aspects that affect the radiation use efficiency. Therefore, by considering the fact that Kermanshah province has favorable condition in terms of more natural resources such as solar radiation, the aims of the present study were evaluation of nitrogen effect on radiation use efficiency, growth indices and yield of some current maize hybrids. Materials and Methods A split plot experiment was done based on randomized complete block design with 4 replications at 2014. Treatments were 4 levels of nitrogen fertilizer application (40%, 70%, 100% and 140% of the maize demand to nitrogen which based on the amount recommended by soil experiment equivalent to 138, 238, 350 and 483 kg.ha-1 of urea as main plots and 3 maize hybrids KSC-704, BC-678 and Simon as sub plots. Leaf area index and total dry matter yield measured during growing season. Crop growth rate and relative growth ratio calculated by differentiation from fitted equation on total dry matter yield data. In order to calculate radiation use efficiency, sunny hours for Kermanshah latitude obtained from the nearest weather station. Daily solar radiation simulated by the method cited by Goudriaan and Van Laar (1993 for growing season. The absorbed radiation in each stage obtained through the multiplication simulated

  7. GARLIC — A general purpose atmospheric radiative transfer line-by-line infrared-microwave code: Implementation and evaluation

    International Nuclear Information System (INIS)

    Schreier, Franz; Gimeno García, Sebastián; Hedelt, Pascal; Hess, Michael; Mendrok, Jana; Vasquez, Mayte; Xu, Jian

    2014-01-01

    A suite of programs for high resolution infrared-microwave atmospheric radiative transfer modeling has been developed with emphasis on efficient and reliable numerical algorithms and a modular approach appropriate for simulation and/or retrieval in a variety of applications. The Generic Atmospheric Radiation Line-by-line Infrared Code — GARLIC — is suitable for arbitrary observation geometry, instrumental field-of-view, and line shape. The core of GARLIC's subroutines constitutes the basis of forward models used to implement inversion codes to retrieve atmospheric state parameters from limb and nadir sounding instruments. This paper briefly introduces the physical and mathematical basics of GARLIC and its descendants and continues with an in-depth presentation of various implementation aspects: An optimized Voigt function algorithm combined with a two-grid approach is used to accelerate the line-by-line modeling of molecular cross sections; various quadrature methods are implemented to evaluate the Schwarzschild and Beer integrals; and Jacobians, i.e. derivatives with respect to the unknowns of the atmospheric inverse problem, are implemented by means of automatic differentiation. For an assessment of GARLIC's performance, a comparison of the quadrature methods for solution of the path integral is provided. Verification and validation are demonstrated using intercomparisons with other line-by-line codes and comparisons of synthetic spectra with spectra observed on Earth and from Venus. - Highlights: • High resolution infrared-microwave radiative transfer model. • Discussion of algorithmic and computational aspects. • Jacobians by automatic/algorithmic differentiation. • Performance evaluation by intercomparisons, verification, validation

  8. Radiative transfer in molecular lines

    Science.gov (United States)

    Asensio Ramos, A.; Trujillo Bueno, J.; Cernicharo, J.

    2001-07-01

    The highly convergent iterative methods developed by Trujillo Bueno and Fabiani Bendicho (1995) for radiative transfer (RT) applications are generalized to spherical symmetry with velocity fields. These RT methods are based on Jacobi, Gauss-Seidel (GS), and SOR iteration and they form the basis of a new NLTE multilevel transfer code for atomic and molecular lines. The benchmark tests carried out so far are presented and discussed. The main aim is to develop a number of powerful RT tools for the theoretical interpretation of molecular spectra.

  9. Focusing of cosmic radiation near power lines. A theoretical approach

    International Nuclear Information System (INIS)

    Skedsmo, A.; Vistnes, A.I.

    1997-02-01

    The purpose of this work was to determine if, and to what extent, cosmic radiation can be focused by power lines. As an alternative to experimental measurements, a computer program was developed for simulation of particle trajectories. Starting from given initial values, the cosmic particles trajectories through the electromagnetic field surrounding power lines were simulated. Particular efforts have been made to choose initial values which represent the actual physical condition of the cosmic radiation at ground level. The results show an average decrease in the particle flux density in an area below a power line and a corresponding increased flux between 12 m and 45 m on either side of the centre of the power line. The average shift in flux density is, however, extremely small (less than 0.1%) and probably not measurable with existing detector technology. 11 refs., 4 figs., 2 tabs

  10. Enhanced non-radiative energy transfer in hybrid III-nitride structures

    International Nuclear Information System (INIS)

    Smith, R. M.; Athanasiou, M.; Bai, J.; Liu, B.; Wang, T.

    2015-01-01

    The effect of surface states has been investigated in hybrid organic/inorganic white light emitting structures that employ high efficiency, nearfield non-radiative energy transfer (NRET) coupling. The structures utilize blue emitting InGaN/GaN multiple quantum well (MQW) nanorod arrays to minimize the separation with a yellow emitting F8BT coating. Surface states due to the exposed III-nitride surfaces of the nanostructures are found to reduce the NRET coupling rate. The surface states are passivated by deposition of a silicon nitride layer on the III-nitride nanorod surface leading to reduced surface recombination. A low thickness surface passivation is shown to increase the NRET coupling rate by 4 times compared to an un-passivated hybrid structure. A model is proposed to explain the increased NRET rate for the passivated hybrid structures based on the reduction in surface electron depletion of the passivated InGaN/GaN MQW nanorods surfaces

  11. Solution of the radiative enclosure with a hybrid inverse method

    Energy Technology Data Exchange (ETDEWEB)

    Silva, Rogerio Brittes da; Franca, Francis Henrique Ramos [Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS (Brazil). Dept. de Engenharia Mecanica], E-mail: frfranca@mecanica.ufrgs.br

    2010-07-01

    This work applies the inverse analysis to solve a three-dimensional radiative enclosure - which the surfaces are diffuse-grays - filled with transparent medium. The aim is determine the powers and locations of the heaters to attain both uniform heat flux and temperature on the design surface. A hybrid solution that couples two methods, the generalized extremal optimization (GEO) and the truncated singular value decomposition (TSVD) is proposed. The determination of the heat sources distribution is treated as an optimization problem, by GEO algorithm , whereas the solution of the system of equation, that embodies the Fredholm equation of first kind and therefore is expected to be ill conditioned, is build up through TSVD regularization method. The results show that the hybrid method can lead to a heat flux on the design surface that satisfies the imposed conditions with maximum error of less than 1,10%. The results illustrated the relevance of a hybrid method as a prediction tool. (author)

  12. Application of the inter-line PCR for the analyse of genomic rearrangements in radiation-transformed mammalian cell lines

    International Nuclear Information System (INIS)

    Leibhard, S.; Smida, J.

    1996-01-01

    Repetitive DNA sequences of the LINE-family (long interspersed elements) that are widely distributed among the mammalian genome can be activated or altered by the exposure to ionizing radiation [1]. By the integration at new sites in the genome alterations in the expression of genes that are involved in cell transformation and/or carcinogenesis may occur [2, 3]. A new technique -the inter-LINE PCR - has been developed in order to detect and analyse such genomic rearrangements in radiation-transformed cell lines. From the sites of transformation- or tumour-specific changes in the genome it might be possible to develop new tumour markers for diagnostic purpose. (orig.) [de

  13. Numerical weather prediction (NWP) and hybrid ARMA/ANN model to predict global radiation

    International Nuclear Information System (INIS)

    Voyant, Cyril; Muselli, Marc; Paoli, Christophe; Nivet, Marie-Laure

    2012-01-01

    We propose in this paper an original technique to predict global radiation using a hybrid ARMA/ANN model and data issued from a numerical weather prediction model (NWP). We particularly look at the multi-layer perceptron (MLP). After optimizing our architecture with NWP and endogenous data previously made stationary and using an innovative pre-input layer selection method, we combined it to an ARMA model from a rule based on the analysis of hourly data series. This model has been used to forecast the hourly global radiation for five places in Mediterranean area. Our technique outperforms classical models for all the places. The nRMSE for our hybrid model MLP/ARMA is 14.9% compared to 26.2% for the naïve persistence predictor. Note that in the standalone ANN case the nRMSE is 18.4%. Finally, in order to discuss the reliability of the forecaster outputs, a complementary study concerning the confidence interval of each prediction is proposed. -- Highlights: ► Time series forecasting with hybrid method based on the use of ALADIN numerical weather model, ANN and ARMA. ► Innovative pre-input layer selection method. ► Combination of optimized MLP and ARMA model obtained from a rule based on the analysis of hourly data series. ► Stationarity process (method and control) for the global radiation time series.

  14. Accelerated Senescence and Enhanced Disease Resistance in Hybrid Chlorosis Lines Derived from Interspecific Crosses between Tetraploid Wheat and Aegilops tauschii

    Science.gov (United States)

    Tosa, Yukio; Yoshida, Kentaro; Park, Pyoyun; Takumi, Shigeo

    2015-01-01

    Hybrid chlorosis, a type of hybrid incompatibility, has frequently been reported in inter- and intraspecific crosses of allopolyploid wheat. In a previous study, we reported some types of growth abnormalities such as hybrid necrosis and observed hybrid chlorosis with mild or severe abnormalities in wheat triploids obtained in crosses between tetraploid wheat cultivar Langdon and four Ae. tauschii accessions and in their derived synthetic hexaploids. However, the molecular mechanisms underlying hybrid chlorosis are not well understood. Here, we compared cytology and gene expression in leaves to characterize the abnormal growth in wheat synthetics showing mild and severe chlorosis. In addition, we compared disease resistance to wheat blast fungus. In total 55 and 105 genes related to carbohydrate metabolism and 53 and 89 genes for defense responses were markedly up-regulated in the mild and severe chlorosis lines, respectively. Abnormal chloroplasts formed in the mesophyll cells before the leaves yellowed in the hybrid chlorosis lines. The plants with mild chlorosis showed increased resistance to wheat blast and powdery mildew fungi, although significant differences only in two, third internode length and maturation time, out of the examined agricultural traits were found between the wild type and plants showing mild chlorosis. These observations suggest that senescence might be accelerated in hybrid chlorosis lines of wheat synthetics. Moreover, in wheat synthetics showing mild chlorosis, the negative effects on biomass can be minimized, and they may show substantial fitness under pathogen-polluted conditions. PMID:25806790

  15. Accelerated senescence and enhanced disease resistance in hybrid chlorosis lines derived from interspecific crosses between tetraploid wheat and Aegilops tauschii.

    Directory of Open Access Journals (Sweden)

    Hiroki Nakano

    Full Text Available Hybrid chlorosis, a type of hybrid incompatibility, has frequently been reported in inter- and intraspecific crosses of allopolyploid wheat. In a previous study, we reported some types of growth abnormalities such as hybrid necrosis and observed hybrid chlorosis with mild or severe abnormalities in wheat triploids obtained in crosses between tetraploid wheat cultivar Langdon and four Ae. tauschii accessions and in their derived synthetic hexaploids. However, the molecular mechanisms underlying hybrid chlorosis are not well understood. Here, we compared cytology and gene expression in leaves to characterize the abnormal growth in wheat synthetics showing mild and severe chlorosis. In addition, we compared disease resistance to wheat blast fungus. In total 55 and 105 genes related to carbohydrate metabolism and 53 and 89 genes for defense responses were markedly up-regulated in the mild and severe chlorosis lines, respectively. Abnormal chloroplasts formed in the mesophyll cells before the leaves yellowed in the hybrid chlorosis lines. The plants with mild chlorosis showed increased resistance to wheat blast and powdery mildew fungi, although significant differences only in two, third internode length and maturation time, out of the examined agricultural traits were found between the wild type and plants showing mild chlorosis. These observations suggest that senescence might be accelerated in hybrid chlorosis lines of wheat synthetics. Moreover, in wheat synthetics showing mild chlorosis, the negative effects on biomass can be minimized, and they may show substantial fitness under pathogen-polluted conditions.

  16. GARLIC - A general purpose atmospheric radiative transfer line-by-line infrared-microwave code: Implementation and evaluation

    Science.gov (United States)

    Schreier, Franz; Gimeno García, Sebastián; Hedelt, Pascal; Hess, Michael; Mendrok, Jana; Vasquez, Mayte; Xu, Jian

    2014-04-01

    A suite of programs for high resolution infrared-microwave atmospheric radiative transfer modeling has been developed with emphasis on efficient and reliable numerical algorithms and a modular approach appropriate for simulation and/or retrieval in a variety of applications. The Generic Atmospheric Radiation Line-by-line Infrared Code - GARLIC - is suitable for arbitrary observation geometry, instrumental field-of-view, and line shape. The core of GARLIC's subroutines constitutes the basis of forward models used to implement inversion codes to retrieve atmospheric state parameters from limb and nadir sounding instruments. This paper briefly introduces the physical and mathematical basics of GARLIC and its descendants and continues with an in-depth presentation of various implementation aspects: An optimized Voigt function algorithm combined with a two-grid approach is used to accelerate the line-by-line modeling of molecular cross sections; various quadrature methods are implemented to evaluate the Schwarzschild and Beer integrals; and Jacobians, i.e. derivatives with respect to the unknowns of the atmospheric inverse problem, are implemented by means of automatic differentiation. For an assessment of GARLIC's performance, a comparison of the quadrature methods for solution of the path integral is provided. Verification and validation are demonstrated using intercomparisons with other line-by-line codes and comparisons of synthetic spectra with spectra observed on Earth and from Venus.

  17. An on-chip polarization splitter based on the radiation loss in the bending hybrid plasmonic waveguide structure

    Science.gov (United States)

    Sun, Chengwei; Rong, Kexiu; Gan, Fengyuan; Chu, Saisai; Gong, Qihuang; Chen, Jianjun

    2017-09-01

    Polarization beam splitters (PBSs) are one of the key components in the integrated photonic circuits. To increase the integration density, various complex hybrid plasmonic structures have been numerically designed to shrink the footprints of the PBSs. Here, to decrease the complexity of the small hybrid structures and the difficulty of the hybrid micro-nano fabrications, the radiation losses are utilized to experimentally demonstrate an ultra-small, broadband, and efficient PBS in a simple bending hybrid plasmonic waveguide structure. The hybrid plasmonic waveguide comprising a dielectric strip on the metal surface supports both the transverse-magnetic (TM) and transverse-electric (TE) waveguide modes. Because of the different field confinements, the TE waveguide mode has larger radiation loss than the TM waveguide mode in the bending hybrid strip waveguide. Based on the different radiation losses, the two incident waveguide modes of orthogonal polarization states are efficiently split in the proposed structure with a footprint of only about 2.2 × 2.2 μm2 on chips. Since there is no resonance or interference in the splitting process, the operation bandwidth is as broad as Δλ = 70 nm. Moreover, the utilization of the strongly confined waveguide modes instead of the bulk free-space light (with the spot size of at least a few wavelengths) as the incident source considerably increases the coupling efficiency, resulting in a low insertion loss of <3 dB.

  18. SEURAT: SPH scheme extended with ultraviolet line radiative transfer

    Science.gov (United States)

    Abe, Makito; Suzuki, Hiroyuki; Hasegawa, Kenji; Semelin, Benoit; Yajima, Hidenobu; Umemura, Masayuki

    2018-05-01

    We present a novel Lyman alpha (Ly α) radiative transfer code, SEURAT (SPH scheme Extended with Ultraviolet line RAdiative Transfer), where line scatterings are solved adaptively with the resolution of the smoothed particle hydrodynamics (SPH). The radiative transfer method implemented in SEURAT is based on a Monte Carlo algorithm in which the scattering and absorption by dust are also incorporated. We perform standard test calculations to verify the validity of the code; (i) emergent spectra from a static uniform sphere, (ii) emergent spectra from an expanding uniform sphere, and (iii) escape fraction from a dusty slab. Thereby, we demonstrate that our code solves the {Ly} α radiative transfer with sufficient accuracy. We emphasize that SEURAT can treat the transfer of {Ly} α photons even in highly complex systems that have significantly inhomogeneous density fields. The high adaptivity of SEURAT is desirable to solve the propagation of {Ly} α photons in the interstellar medium of young star-forming galaxies like {Ly} α emitters (LAEs). Thus, SEURAT provides a powerful tool to model the emergent spectra of {Ly} α emission, which can be compared to the observations of LAEs.

  19. Single-shot dual-wavelength in-line and off-axis hybrid digital holography

    Science.gov (United States)

    Wang, Fengpeng; Wang, Dayong; Rong, Lu; Wang, Yunxin; Zhao, Jie

    2018-02-01

    We propose an in-line and off-axis hybrid holographic real-time imaging technique. The in-line and off-axis digital holograms are generated simultaneously by two lasers with different wavelengths, and they are recorded using a color camera with a single shot. The reconstruction is carried using an iterative algorithm in which the initial input is designed to include the intensity of the in-line hologram and the approximate phase distributions obtained from the off-axis hologram. In this way, the complex field in the object plane and the output by the iterative procedure can produce higher quality amplitude and phase images compared to traditional iterative phase retrieval. The performance of the technique has been demonstrated by acquiring the amplitude and phase images of a green lacewing's wing and a living moon jellyfish.

  20. Super-radiant Smith–Purcell radiation from periodic line charges

    International Nuclear Information System (INIS)

    Li, D.; Hangyo, M.; Tsunawaki, Y.; Yang, Z.; Wei, Y.; Miyamoto, S; Asakawa, M.R.; Imasaki, K.

    2012-01-01

    Smith–Purcell radiation occurs when an electron passes close to the surface of a metallic grating. The radiation becomes coherent when the length of the electron bunch is smaller than the wavelength of the radiation. A train of periodic bunches can enhance the spectral intensity by changing the angular and spectral distribution of the radiation. This is called super-radiant Smith–Purcell radiation, and has been observed in experiments and particle-in-cell simulations. In this paper, we introduce a new method to study this effect by calculating the reflected waves of an incident evanescent wave from periodic line charges. The reflection coefficients are numerically computed, and the spectral distributions of the super-radiant radiation are demonstrated. These analytical results are in agreement with those obtained through part-in-cell simulations.

  1. Utilization of gamma radiation for the induction of phenotypical variability in hybrid petunia cultivated in vitro

    International Nuclear Information System (INIS)

    Miranda Jimenez, Javier I.

    2006-01-01

    Conditions of in vitro culture of two lines of yellow petunia (hybrid petunia) were studied. The effect of two controllers of growth in the establishment was evaluated. It was observed that the lines improved their growth in the absence of BAP (6-benzil amino purine) and with addition of AIA (3-indoleacetic acid) in doses of 0,5 to 2 mg 1 - 1 1 (milligrams by litre). The BAP in the used concentrations (0, 1, 0,2, 0,5 mg 1 -1 ), produced toxic effects to the plantlets growth, presented as hyperhydricity and backwardness. Every line answered better to a different combination of regulators. Therefore, it was defined for the meristems sowing the use of a MS means (Murashige and Skoog, 1962) without regulators for the AMW3A line and a MS means with addition of 2 mg 1 -1 of AIA for the YELLOWAAS line. A radio sensibility curve for the AMW3A line was established. The irradiation process was continued only with the AMW3A line because this is the principal cause of the commercial hybrids that are proving in the country. The mortality increase followed to a lineal model with the increase of the gamma radiation doses was observed. It was established that lethal media doses was of 3 krad. Criteria of surviving and growth of irradiated material was used and it was selected a doses of 2 krad for treatment of a more quantity of plantlets. The growth, with respect to witness, was reduced to half to the four weeks of the irradiation and did not make root. Plantlets with deformation of leafs and chlorophyllous chimeras were observed. The plants that survived to the irradiation process were multiplied and, later, they were acclimatized in greenhouse conditions. An visual analyses of the morphological variations was performed, and the possible phenotypical variation of these plants were determined. Modifications in the form of the plant, the leaf and the flower were found. Nevertheless, the presence of some of these variations in the witness plants suggests that much of the variations

  2. Radiation equivalence of genotoxic chemicals - Validation in cultered mammalian cell lines

    International Nuclear Information System (INIS)

    Murthy, M.S.S.

    1982-01-01

    Published data on mutations induced by ionizing radiation and 6 monofunctional alkylating agents, namely EMS, MMS, ENNG, MNNG, ENU and MNU, in different cell lines (Chinese hamster ovary, Chinese hamster lung V79, mouse lymphoma L5178 and human cells) were analysed so that radiation-equivalent chemical (REC) values could be calculated. REC values thus obtained for a given alkylating agent with different cell lines fall within a narrow range suggesting its validation in cultured mammalian cell systems including human. (orig.)

  3. Hybrid breeding in pepper (capsicum annuum L. ). [Gamma and x radiation

    Energy Technology Data Exchange (ETDEWEB)

    Milkova, L; Daskalov, S [Akademiya na Selskostopanskite Nauki, Sofia (Bulgaria). Inst. po Genetika

    1981-01-01

    Male sterile forms of peppers with gene sterility were produced by ..gamma..- and X-irradiation of dry seeds of cv. Pazarjishka kapiya 794 and Zlaten medal and subsequent selection in M/sub 2/. Male sterile lines suitable for developing hybrid cultivars intended for various kinds of production were obtained by backcrossing with lines and cultivars having valuable economic characters and high combining ability. The promising pepper Belasitsa, Prista, Lyulin and Strouma (all of them for early field production), and Izoumroud and Prevuzhoden (for glasshouse production) are described.

  4. Field screening of experimental corn hybrids and inbred lines for multiple ear-feeding insect resistance.

    Science.gov (United States)

    Ni, Xinzhi; Xu, Wenwei; Krakowsky, Matthew D; Buntin, G David; Brown, Steve L; Lee, R Dewey; Coy, Anton E

    2007-10-01

    Identifying and using native insect resistance genes is the core of integrated pest management. In this study, 10 experimental corn, Zea mays L., hybrids and 10 inbred lines were screened for resistance to major ear-feeding insects in the southeastern Coastal Plain region of the United States during 2004 and 2005. Ear-feeding insect damage was assessed at harvest by visual damage rating for the corn earworm, Helicoverpa zea (Boddie), and by the percentage of kernels damaged by the maize weevil, Sitophilus zeamais Motschulsky, and stink bugs [combination of Euschistus servus (Say) and southern green stink bug, Nezara viridula (L.)]. Among the eight inbred lines and two control populations examined, C3S1B73-5b was resistant to corn earworm, maize weevil, and stink bugs. In contrast, C3S1B73-4 was resistant to corn earworm and stink bugs, but not to maize weevil. In a similar manner, the corn hybrid S1W*CML343 was resistant to all three ear-feeding insects, whereas hybrid C3S1B73-3*Tx205 was resistant to corn earworm and maize weevil in both growing seasons, but susceptible to stink bugs in 2005. The silk-feeding bioassay showed that corn earworm developed better on corn silk than did fall armyworm. Among all phenotypic traits examined (i.e., corn ear size, husk extension, and husk tightness), only corn ear size was negatively correlated to corn earworm damage in the inbred lines examined, whereas only husk extension (i.e., coverage) was negatively correlated to both corn earworm and maize weevil damage on the experimental hybrids examined. Such information could be used to establish a baseline for developing agronomically elite corn germplasm that confers multiple ear-feeding insect resistance.

  5. γ-radiation induces cellular sensitivity and aberrant methylation in human tumor cell lines.

    Science.gov (United States)

    Kumar, Ashok; Rai, Padmalatha S; Upadhya, Raghavendra; Vishwanatha; Prasada, K Shama; Rao, B S Satish; Satyamoorthy, Kapettu

    2011-11-01

    Ionizing radiation induces cellular damage through both direct and indirect mechanisms, which may include effects from epigenetic changes. The purpose of this study was to determine the effect of ionizing radiation on DNA methylation patterns that may be associated with altered gene expression. Sixteen human tumor cell lines originating from various cancers were initially tested for radiation sensitivity by irradiating them with γ-radiation in vitro and subsequently, radiation sensitive and resistant cell lines were treated with different doses of a demethylating agent, 5-Aza-2'-Deoxycytidine (5-aza-dC) and a chromatin modifier, Trichostatin-A (TSA). Survival of these cell lines was measured using 3-(4, 5-Dimethylthiazol- 2-yl)-2, 5-diphenyltetrazolium (MTT) and clonogenic assays. The effect of radiation on global DNA methylation was measured using reverse phase high performance liquid chromatography (RP-HPLC). The transcription response of methylated gene promoters, from cyclin-dependent kinase inhibitor 2A (p16(INK4a)) and ataxia telangiectasia mutated (ATM) genes, to radiation was measured using a luciferase reporter assay. γ-radiation resistant (SiHa and MDAMB453) and sensitive (SaOS2 and WM115) tumor cell lines were examined for the relationship between radiation sensitivity and DNA methylation. Treatment of cells with 5-aza-dC and TSA prior to irradiation enhanced DNA strand breaks, G2/M phase arrest, apoptosis and cell death. Exposure to γ-radiation led to global demethylation in a time-dependent manner in tumor cells in relation to resistance and sensitivity to radiation with concomitant activation of p16(INK4a) and ATM gene promoters. These results provide important information on alterations in DNA methylation as one of the determinants of radiation effects, which may be associated with altered gene expression. Our results may help in delineating the mechanisms of radiation resistance in tumor cells, which can influence diagnosis, prognosis and

  6. Performance comparison of a new hybrid conjugate gradient method under exact and inexact line searches

    Science.gov (United States)

    Ghani, N. H. A.; Mohamed, N. S.; Zull, N.; Shoid, S.; Rivaie, M.; Mamat, M.

    2017-09-01

    Conjugate gradient (CG) method is one of iterative techniques prominently used in solving unconstrained optimization problems due to its simplicity, low memory storage, and good convergence analysis. This paper presents a new hybrid conjugate gradient method, named NRM1 method. The method is analyzed under the exact and inexact line searches in given conditions. Theoretically, proofs show that the NRM1 method satisfies the sufficient descent condition with both line searches. The computational result indicates that NRM1 method is capable in solving the standard unconstrained optimization problems used. On the other hand, the NRM1 method performs better under inexact line search compared with exact line search.

  7. Endogenous superoxide dismutase and catalase activities and radiation resistance in mouse cell lines

    International Nuclear Information System (INIS)

    Davy, C.A.; Tesfay, Z.; Jones, J.; Rosenberg, R.C.; McCarthy, C.; Ostrand-Rosenberg, S.

    1988-01-01

    The relationship between the endogenous cytoplasmic levels of the enzymes superoxide dismutase and catalase and the inhibition of cell proliferation by γ-radiation has been studied in 11 mouse cell lines. The resistance of these mouse cell lines to radiation was found to vary by over 25-fold. No correlation was found between the cytoplasmic level of CuZn-superoxide dismutase or catalase and the resistance to radiation as measured by extrapolation number (EN), quasi-threshold dose (Dsub(q)), or Dsub(o). None of the cell lines had detectable cytoplasmic Mn-superoxide dismutase. The apparent Ksub(i) of potassium cyanide for mouse CuZn-superoxide dismutase was determined (Ksub(i) = 6.5 μmol dm -3 ). (author)

  8. Effect of radiation combined with hyperthermia on human prostatic carcinoma cell lines in culture

    International Nuclear Information System (INIS)

    Kaver, I.; Ware, J.L.; Wilson, J.D.; Koontz, W.W. Jr.

    1991-01-01

    The effect of radiation combined with heat on three human prostatic carcinoma cell lines growing in vitro was investigated. Cells were exposed to different radiation doses followed by heat treatment at 43 degrees C for one hour. Heat treatment, given ten minutes after radiation, significantly enhanced the radiation response of all the cell lines studied. The combined effect of radiation and heat produced greater cytotoxicity than predicted from the additive effects of the two individual treatment modalities alone. These results indicate that a combined treatment regimen of radiation plus hyperthermia (43 degrees, 1 hr) might be an important tool in maintaining a better local control of prostatic cancer

  9. Simultaneous reconstruction of temperature distribution and radiative properties in participating media using a hybrid LSQR-PSO algorithm

    Science.gov (United States)

    Niu, Chun-Yang; Qi, Hong; Huang, Xing; Ruan, Li-Ming; Wang, Wei; Tan, He-Ping

    2015-11-01

    A hybrid least-square QR decomposition (LSQR)-particle swarm optimization (LSQR-PSO) algorithm was developed to estimate the three-dimensional (3D) temperature distributions and absorption coefficients simultaneously. The outgoing radiative intensities at the boundary surface of the absorbing media were simulated by the line-of-sight (LOS) method, which served as the input for the inverse analysis. The retrieval results showed that the 3D temperature distributions of the participating media with known radiative properties could be retrieved accurately using the LSQR algorithm, even with noisy data. For the participating media with unknown radiative properties, the 3D temperature distributions and absorption coefficients could be retrieved accurately using the LSQR-PSO algorithm even with measurement errors. It was also found that the temperature field could be estimated more accurately than the absorption coefficients. In order to gain insight into the effects on the accuracy of temperature distribution reconstruction, the selection of the detection direction and the angle between two detection directions was also analyzed. Project supported by the Major National Scientific Instruments and Equipment Development Special Foundation of China (Grant No. 51327803), the National Natural Science Foundation of China (Grant No. 51476043), and the Fund of Tianjin Key Laboratory of Civil Aircraft Airworthiness and Maintenance in Civil Aviation University of China.

  10. The UF family of hybrid phantoms of the developing human fetus for computational radiation dosimetry

    International Nuclear Information System (INIS)

    Maynard, Matthew R; Geyer, John W; Bolch, Wesley; Aris, John P; Shifrin, Roger Y

    2011-01-01

    Historically, the development of computational phantoms for radiation dosimetry has primarily been directed at capturing and representing adult and pediatric anatomy, with less emphasis devoted to models of the human fetus. As concern grows over possible radiation-induced cancers from medical and non-medical exposures of the pregnant female, the need to better quantify fetal radiation doses, particularly at the organ-level, also increases. Studies such as the European Union's SOLO (Epidemiological Studies of Exposed Southern Urals Populations) hope to improve our understanding of cancer risks following chronic in utero radiation exposure. For projects such as SOLO, currently available fetal anatomic models do not provide sufficient anatomical detail for organ-level dose assessment. To address this need, two fetal hybrid computational phantoms were constructed using high-quality magnetic resonance imaging and computed tomography image sets obtained for two well-preserved fetal specimens aged 11.5 and 21 weeks post-conception. Individual soft tissue organs, bone sites and outer body contours were segmented from these images using 3D-DOCTOR(TM) and then imported to the 3D modeling software package Rhinoceros(TM) for further modeling and conversion of soft tissue organs, certain bone sites and outer body contours to deformable non-uniform rational B-spline surfaces. The two specimen-specific phantoms, along with a modified version of the 38 week UF hybrid newborn phantom, comprised a set of base phantoms from which a series of hybrid computational phantoms was derived for fetal ages 8, 10, 15, 20, 25, 30, 35 and 38 weeks post-conception. The methodology used to construct the series of phantoms accounted for the following age-dependent parameters: (1) variations in skeletal size and proportion, (2) bone-dependent variations in relative levels of bone growth, (3) variations in individual organ masses and total fetal masses and (4) statistical percentile variations in

  11. Effects of hyperthermia and ionizing radiation in normal and ataxia telangiectasia human fibroblast lines

    International Nuclear Information System (INIS)

    Mitchel, R.E.J.; Chan, A.; Smith, B.P.; Child, S.D.; Paterson, M.C.

    1984-01-01

    The effects of 45 0 C hyperthermia and γ radiation have been studied in three normal human fibroblast lines (GM38, GM730, WI38) and compared to the effects in two lines derived from patients with the hereditary disease ataxia telangiectasia (AR3BI, AT5BI). All lines, both normal and γ-sensitive AT, showed a similar resistance to killing by heat alone, suggesting that the defect responsible for the increased radiation sensitivity in AT lines does not confer increased heat sensitivity. Shouldered survival curves were obtained in each case indicating the ability to accumulate sublethal heat damage. All normal and AT cell lines exhibited increased resistance to the lethal effects of heat in response to a thermal stress, indicating that the defect that causes radiosensitivity in AT cell lines does not prevent the induction of thermotolerance. It was hypothesized that in normal cells, this heat treatment inactivates the process which is already defective in AT lines, and that this process may be required for the proper rejoining of double-strand breaks produced during the repair of other radiation-induced lesions

  12. Reduction of the electromagnetic radiation in high tension lines

    International Nuclear Information System (INIS)

    Araya Padilla, Eddie A.

    2006-01-01

    Introduces like the design of the transmission lines determine the profile of the electric and magnetic generated field. This profile is calculated with the use of the equations of Maxwell, and it depends rely on diverse geometries and operative factors of the line, so that an adequate physical distribution of the tower configuration, it could diminish the intensity of radiation generated inside of and in the border of right of way of the line. Pattern like the electric and magnetic generated fields are distributed satisfactorily, by means of the analysis of sensibility of parameters of design. (author) [es

  13. Radiation from a Slot System in the Coaxial Line Shield

    Science.gov (United States)

    Katrich, V. A.; Lyashchenko, V. A.; Medvedev, N. V.

    2012-06-01

    The problem of electromagnetic wave excitation, scattering and radiation by the system of transverse slots, cut in the outer conductor of an infinite coaxial line, is solved by the magnetomotive forces method. The radiation and reflection coefficients of the circular and arc slot systems are investigated in dependence on slot sizes and feeder parameters. The processes of radiation into lossy material media are studied. The researches have been carried out with the interconnection between slots of internal and external regions considered.

  14. Test-beam Results from a RICH Detector Prototype Using Aerogel Radiator and Pixel Hybrid Photon Detectors

    CERN Document Server

    Aglieri-Rinella, G; Van Lysebetten, A; Piedigrossi, D; Wyllie, K; Bellunato, T F; Calvi, M; Matteuzzi, C; Musy, M; Perego, D L; Somerville, L P; Newby, C; Easo, S; Wotton, S

    2006-01-01

    A test-beam study was performed at CERN with a Ring Imaging Cherenkov (RICH) prototype using three pixel Hybrid Photon Detectors. Results on the photon yield and Cherenkov angle resolution are presented here, for the Aerogel radiator and also for reference runs taken with Nitrogen radiator.

  15. Transpiration Response and Growth in Pearl Millet Parental Lines and Hybrids Bred for Contrasting Rainfall Environments

    Directory of Open Access Journals (Sweden)

    Susan Medina

    2017-10-01

    Full Text Available Under conditions of high vapor pressure deficit (VPD and soil drying, restricting transpiration is an important avenue to gain efficiency in water use. The question we raise in this article is whether breeding for agro-ecological environments that differ for the rainfall have selected for traits that control plant water use. These are measured in pearl millet materials bred for zones varying in rainfall (8 combinations of parent and F1-hybrids, 18 F1-hybrids and then 40 F1-hybrids. In all cases, we found an agro-ecological variation in the slope of the transpiration response to increasing VPD, and parental line variation in the transpiration response to soil drying within hybrids/parent combinations. The hybrids adapted to lower rainfall had higher transpiration response curves than those from the highest rainfall zones, but showed no variation in how transpiration responded to soil drying. The genotypes bred for lower rainfall zones showed lower leaf area, dry matter, thicker leaves, root development, and exudation, than the ones bred for high rainfall zone when grown in the low VPD environment of the greenhouse, but there was no difference in their root length neither on the root/shoot index in these genotypes. By contrast, when grown under high VPD conditions outdoors, the lower rainfall hybrids had the highest leaf, tiller, and biomass development. Finally, under soil drying the genotypes from the lower rainfall accumulated less biomass than the ones from higher rainfall zone, and so did the parental lines compared to the hybrids. These differences in the transpiration response and growth clearly showed that breeding for different agro-ecological zones also bred for different genotype strategies in relation to traits related to plant water use.Highlights:• Variation in transpiration response reflected breeding for agro-ecological zones• Different growth strategies depended on the environmental conditions• Different ideotypes reflected

  16. Transpiration Response and Growth in Pearl Millet Parental Lines and Hybrids Bred for Contrasting Rainfall Environments.

    Science.gov (United States)

    Medina, Susan; Gupta, S K; Vadez, Vincent

    2017-01-01

    Under conditions of high vapor pressure deficit (VPD) and soil drying, restricting transpiration is an important avenue to gain efficiency in water use. The question we raise in this article is whether breeding for agro-ecological environments that differ for the rainfall have selected for traits that control plant water use. These are measured in pearl millet materials bred for zones varying in rainfall (8 combinations of parent and F 1 -hybrids, 18 F 1 -hybrids and then 40 F 1 -hybrids). In all cases, we found an agro-ecological variation in the slope of the transpiration response to increasing VPD, and parental line variation in the transpiration response to soil drying within hybrids/parent combinations. The hybrids adapted to lower rainfall had higher transpiration response curves than those from the highest rainfall zones, but showed no variation in how transpiration responded to soil drying. The genotypes bred for lower rainfall zones showed lower leaf area, dry matter, thicker leaves, root development, and exudation, than the ones bred for high rainfall zone when grown in the low VPD environment of the greenhouse, but there was no difference in their root length neither on the root/shoot index in these genotypes. By contrast, when grown under high VPD conditions outdoors, the lower rainfall hybrids had the highest leaf, tiller, and biomass development. Finally, under soil drying the genotypes from the lower rainfall accumulated less biomass than the ones from higher rainfall zone, and so did the parental lines compared to the hybrids. These differences in the transpiration response and growth clearly showed that breeding for different agro-ecological zones also bred for different genotype strategies in relation to traits related to plant water use. Highlights : • Variation in transpiration response reflected breeding for agro-ecological zones • Different growth strategies depended on the environmental conditions • Different ideotypes reflected

  17. Gamma-ray lines from radiative dark matter decay

    International Nuclear Information System (INIS)

    Garny, Mathias; Ibarra, Alejandro; Tran, David; Weniger, Christoph

    2011-01-01

    The decay of dark matter particles which are coupled predominantly to charged leptons has been proposed as a possible origin of excess high-energy positrons and electrons observed by cosmic-ray telescopes PAMELA and Fermi LAT. Even though the dark matter itself is electrically neutral, the tree-level decay of dark matter into charged lepton pairs will generically induce radiative two-body decays of dark matter at the quantum level. Using an effective theory of leptophilic dark matter decay, we calculate the rates of radiative two-body decays for scalar and fermionic dark matter particles. Due to the absence of astrophysical sources of monochromatic gamma rays, the observation of a line in the diffuse gamma-ray spectrum would constitute a strong indication of a particle physics origin of these photons. We estimate the intensity of the gamma-ray line that may be present in the energy range of a few TeV if the dark matter decay interpretation of the leptonic cosmic-ray anomalies is correct and comment on observational prospects of present and future Imaging Cherenkov Telescopes, in particular the CTA

  18. Selection and characterizations of radiation-induced salinity-tolerant lines in rice

    International Nuclear Information System (INIS)

    Lee, I.S.; Kim, D.S.; Lee, S.J.; Song, H.S.; Lim, Y.P.; Lee, Y.I.

    2003-01-01

    NaCl tolerant cell lines were selected from irradiated callus, which were generated from seed cultures. M 1 -regenerates were obtained from the salt-tolerant callus cultured on the auxin-free medium for 30 days. Some regenerants were more tolerant than the parent variety (Dongjinbyeo) on a medium containing 0.75 % NaCl. Seeds (M 3 5,000 lines) derived from M 2 lines were grown to the 3 leaf stage. M 3 lines were soaked with a 0.75 % salt solution for 3 weeks and 350 salt-tolerant genotypes were selected. Among the M 3 350 lines, forty tolerant lines were selected from a saline field (10~14 mS) near the sea coast. Of the forty lines, two lines (18-1 and 50-1) showed more improved plant height, panicle length, tillering number, spikelet number and yield than those of the original variety. Thirty primers were screened and two RAPD markers were identified, which appeared in both the salt-tolerant lines (18-1 and 50-1). From DNA-hybridization experiments, it appeared that the fragment arose from the middle-repetitive copy sequences. The transcript involved in the marker showed a higher expression in the salt-tolerant lines than the sensitive lines. The salt-tolerant lines would be useful as a resource for salt-tolerant breeding. (author)

  19. Electromagnetic radiation by parametric decay of upper hybrid waves in ionospheric modification experiments

    International Nuclear Information System (INIS)

    Leyser, T.B.

    1994-01-01

    A nonlinear dispersion relation for the parametric decay of an electrostatic upper hybrid wave into an ordinary mode electromagnetic wave, propagating parallel to the ambient magnetic field, and an electrostatic low frequency wave, being either a lower hybrid wave or a high harmonic ion Bernstein wave, is derived. The coherent and resonant wave interaction is considered to take place in a weakly magnetized and collisionless Vlasov plasma. The instability growth rate is computed for parameter values typical of ionospheric modification experiments, in which a powerful high frequency electromagnetic pump wave is injected into the ionospheric F-region from ground-based transmitters. The electromagnetic radiation which is excited by the decaying upper hybrid wave is found to be consistent with the prominent and commonly observed downshifted maximum (DM) emission in the spectrum of stimulated electromagnetic emission

  20. Cadmium accumulation characteristics of low-cadmium rice (Oryza sativa L.) line and F1 hybrids grown in cadmium-contaminated soils.

    Science.gov (United States)

    Li, Kun; Yu, Haiying; Li, Tingxuan; Chen, Guangdeng; Huang, Fu

    2017-07-01

    Cadmium (Cd) pollution has threatened severely to food safety and human health. A pot experiment and a field experiment were conducted to investigate the difference of Cd accumulation between rice (Oryza sativa L.) lines and F 1 hybrids in Cd-contaminated soils. The adverse effect on biomass of rice lines was greater than that of F 1 hybrids under Cd treatments in the pot experiment. The variations of Cd concentration among rice cultivars in different organs were smaller in stem and leaf, but larger in root and ear. Average proportion of Cd in root of F 1 hybrids was 1.39, 1.39, and 1.16 times higher than those of rice lines at the treatment of 1, 2, and 4 mg Cd kg -1 soil, respectively. Cd concentrations in ear of F 1 hybrids were significantly lower than rice lines with the reduction from 29.24 to 50.59%. Cd concentrations in brown rice of all F 1 hybrids were less than 0.2 mg kg -1 at 1 mg Cd kg -1 soil, in which Lu98A/YaHui2816, 5406A/YaHui2816, and C268A/YaHui2816 could be screened out as cadmium-safe cultivars (CSCs) for being safe even at 2 mg Cd kg -1 soil. C268A/YaHui2816 showed the lowest Cd concentration in root among F 1 hybrids, while Lu98A/YaHui2816 and 5406A/YaHui2816 showed lower capability of Cd translocation from root to shoot under Cd exposure, which eventually caused the lower Cd accumulation in brown rice. The lower level of Cd translocation contributed to reducing the accumulation of Cd in brown rice had been validated by the field experiment. Thus, Lu98A/YaHui2816, 5406A/YaHui2816, and C268A/YaHui2816 could be considered as potential CSCs to cultivate in Cd-contaminated soils (<2 mg Cd kg -1 soil).

  1. Phenology, sterility and inheritance of two environment genic male sterile (EGMS) lines for hybrid rice.

    Science.gov (United States)

    El-Namaky, R; van Oort, P A J

    2017-12-01

    There is still limited quantitative understanding of how environmental factors affect sterility of Environment-conditioned genic male sterility (EGMS) lines. A model was developed for this purpose and tested based on experimental data from Ndiaye (Senegal) in 2013-2015. For the two EGMS lines tested here, it was not clear if one or more recessive gene(s) were causing male sterility. This was tested by studying sterility segregation of the F2 populations. Daylength (photoperiod) and minimum temperatures during the period from panicle initiation to flowering had significant effects on male sterility. Results clearly showed that only one recessive gene was involved in causing male sterility. The model was applied to determine the set of sowing dates of two different EGMS lines such that both would flower at the same time the pollen would be completely sterile. In the same time the local popular variety (Sahel 108, the male pollen donor) being sufficiently fertile to produce the hybrid seeds. The model was applied to investigate the viability of the two line breeding system in the same location with climate change (+2oC) and in two other potential locations: in M'Be in Ivory Coast and in the Nile delta in Egypt. Apart from giving new insights in the relation between environment and EGMS, this study shows that these insights can be used to assess safe sowing windows and assess the suitability of sterility and fertility period of different environments for a two line hybrid rice production system.

  2. Determination of High-Frequency Current Distribution Using EMTP-Based Transmission Line Models with Resulting Radiated Electromagnetic Fields

    Energy Technology Data Exchange (ETDEWEB)

    Mork, B; Nelson, R; Kirkendall, B; Stenvig, N

    2009-11-30

    Application of BPL technologies to existing overhead high-voltage power lines would benefit greatly from improved simulation tools capable of predicting performance - such as the electromagnetic fields radiated from such lines. Existing EMTP-based frequency-dependent line models are attractive since their parameters are derived from physical design dimensions which are easily obtained. However, to calculate the radiated electromagnetic fields, detailed current distributions need to be determined. This paper presents a method of using EMTP line models to determine the current distribution on the lines, as well as a technique for using these current distributions to determine the radiated electromagnetic fields.

  3. Floquet Weyl semimetals in light-irradiated type-II and hybrid line-node semimetals

    Science.gov (United States)

    Chen, Rui; Zhou, Bin; Xu, Dong-Hui

    2018-04-01

    Type-II Weyl semimetals have recently attracted intensive research interest because they host Lorentz-violating Weyl fermions as quasiparticles. The discovery of type-II Weyl semimetals evokes the study of type-II line-node semimetals (LNSMs) whose linear dispersion is strongly tilted near the nodal ring. We present here a study on the circularly polarized light-induced Floquet states in type-II LNSMs, as well as those in hybrid LNSMs that have a partially overtilted linear dispersion in the vicinity of the nodal ring. We illustrate that two distinct types of Floquet Weyl semimetal (WSM) states can be induced in periodically driven type-II and hybrid LNSMs, and the type of Floquet WSMs can be tuned by the direction and intensity of the incident light. We construct phase diagrams of light-irradiated type-II and hybrid LNSMs which are quite distinct from those of light-irradiated type-I LNSMs. Moreover, we show that photoinduced Floquet type-I and type-II WSMs can be characterized by the emergence of different anomalous Hall conductivities.

  4. Overcoming inter-subspecific hybrid sterility in rice by developing indica-compatible japonica lines.

    Science.gov (United States)

    Guo, Jie; Xu, Xiaomei; Li, Wentao; Zhu, Wenyin; Zhu, Haitao; Liu, Ziqiang; Luan, Xin; Dai, Ziju; Liu, Guifu; Zhang, Zemin; Zeng, Ruizhen; Tang, Guang; Fu, Xuelin; Wang, Shaokui; Zhang, Guiquan

    2016-06-01

    Rice (Oryza sativa L.) is an important staple crop. The exploitation of the great heterosis that exists in the inter-subspecific crosses between the indica and japonica rice has long been considered as a promising way to increase the yield potential. However, the male and female sterility frequently occurred in the inter-subspecific hybrids hampered the utilization of the heterosis. Here we report that the inter-subspecific hybrid sterility in rice is mainly affected by the genes at Sb, Sc, Sd and Se loci for F1 male sterility and the gene at S5 locus for F1 female sterility. The indica-compatible japonica lines (ICJLs) developed by pyramiding the indica allele (S-i) at Sb, Sc, Sd and Se loci and the neutral allele (S-n) at S5 locus in japonica genetic background through marker-assisted selection are compatible with indica rice in pollen fertility and in spikelet fertility. These results showed a great promise of overcoming the inter-subspecific hybrid sterility and exploiting the heterosis by developing ICJLs.

  5. Radiation response of haematopoietic cell lines of human origin

    International Nuclear Information System (INIS)

    Lehnert, S.; Rybka, W.B.; Suissa, S.; Giambattisto, D.

    1986-01-01

    Six human haematopoietic cell lines, five of leukaemic origin, including cells with myeloid, lymphoid and undifferentiated phenotype have been studied with respect to radiation response. The intrinsic radio-sensitivity of the cells varied widely, the D 0 s ranging from 0.53 to 1.39 Gy. Five of the cell lines showed some capacity to accumulate sublethal damage; in three of these, enhanced survival was demonstrated in split-dose experiments. One cell line (HL-60) was anomalous in that although little accumulation of sublethal damage was demonstrable, survival was enhanced by fractionation of the dose. Five of the six cell lines studied were of leukaemic origin. The results support the belief that, in contrast to the almost constant radiosensitivity of normal haematopoietic cell progenitors, leukaemic cell progenitors may show a wide range of radiosensitivities. (author)

  6. The UF family of hybrid phantoms of the developing human fetus for computational radiation dosimetry

    Energy Technology Data Exchange (ETDEWEB)

    Maynard, Matthew R; Geyer, John W; Bolch, Wesley [Department of Nuclear and Radiological Engineering, University of Florida, Gainesville, FL (United States); Aris, John P [Department of Anatomy and Cell Biology, University of Florida, Gainesville, FL (United States); Shifrin, Roger Y, E-mail: wbolch@ufl.edu [Department of Radiology, University of Florida, Gainesville, FL (United States)

    2011-08-07

    Historically, the development of computational phantoms for radiation dosimetry has primarily been directed at capturing and representing adult and pediatric anatomy, with less emphasis devoted to models of the human fetus. As concern grows over possible radiation-induced cancers from medical and non-medical exposures of the pregnant female, the need to better quantify fetal radiation doses, particularly at the organ-level, also increases. Studies such as the European Union's SOLO (Epidemiological Studies of Exposed Southern Urals Populations) hope to improve our understanding of cancer risks following chronic in utero radiation exposure. For projects such as SOLO, currently available fetal anatomic models do not provide sufficient anatomical detail for organ-level dose assessment. To address this need, two fetal hybrid computational phantoms were constructed using high-quality magnetic resonance imaging and computed tomography image sets obtained for two well-preserved fetal specimens aged 11.5 and 21 weeks post-conception. Individual soft tissue organs, bone sites and outer body contours were segmented from these images using 3D-DOCTOR(TM) and then imported to the 3D modeling software package Rhinoceros(TM) for further modeling and conversion of soft tissue organs, certain bone sites and outer body contours to deformable non-uniform rational B-spline surfaces. The two specimen-specific phantoms, along with a modified version of the 38 week UF hybrid newborn phantom, comprised a set of base phantoms from which a series of hybrid computational phantoms was derived for fetal ages 8, 10, 15, 20, 25, 30, 35 and 38 weeks post-conception. The methodology used to construct the series of phantoms accounted for the following age-dependent parameters: (1) variations in skeletal size and proportion, (2) bone-dependent variations in relative levels of bone growth, (3) variations in individual organ masses and total fetal masses and (4) statistical percentile variations

  7. Differential responses to radiation and hyperthermia of cloned cell lines derived from a single human melanoma xenograft

    International Nuclear Information System (INIS)

    Rofstad, E.K.; Brustad, T.

    1984-01-01

    One uncloned and five cloned cell lines were derived from a single human melanoma xenograft. Cells from passages 7-12 were exposed to either radiation or hyperthermia (42.5 0 C, pH = 7.4) under aerobic conditions and the colony forming ability of the cells was assayed in soft agar. The five cloned lines showed individual and characteristic responses to radiation as well as to hyperthermia. The variation in the response to radiation was mainly reflected in the size of the shoulders of the survival curves rather than in the D 0 -values. The variation in the response to hyperthermia was mainly reflected in the terminal slopes of the survival curves. The survival curve of cells from the uncloned line, both when exposed to radiation and hyperthermia, was positioned in the midst of those of the cloned lines. The response of the cloned lines to radiation did not correlate with the response to hyperthermia, indicating that tumor cell subpopulations which are resistant to radiation may respond well to hyperthermia

  8. Delay-Line Three-Dimensional Position Sensitive Radiation Detection

    Science.gov (United States)

    Jeong, Manhee

    High-resistivity silicon(Si) in large volumes and with good charge carrier transport properties has been produced and achieved success as a radiation detector material over the past few years due to its relatively low cost as well as the availability of well-established processing technologies. One application of that technology is in the fabrication of various position-sensing topologies from which the incident radiation's direction can be determined. We have succeeded in developing the modeling tools for investigating different position-sensing schemes and used those tools to examine both amplitude-based and time-based methods, an assessment that indicates that fine position-sensing can be achieved with simpler readout designs than are conventionally deployed. This realization can make ubiquitous and inexpensive deployment of special nuclear materials (SNM) detecting technology becomes more feasible because if one can deploy position-sensitive semiconductor detectors with only one or two contacts per side. For this purpose, we have described the delay-line radiation detector and its optimized fabrication. The semiconductor physics were simulated, the results from which guided the fabrication of the guard ring structure and the detector electrode, both of which included metal-field-plates. The measured improvement in the leakage current was confirmed with the fabricated devices, and the structures successfully suppressed soft-breakdown. We also demonstrated that fabricating an asymmetric strip-line structure successfully minimizing the pulse shaping and increases the distance through which one can propagate the information of the deposited charge distribution. With fabricated delay-line detectors we can acquire alpha spectra (Am-241) and gamma spectra (Ba-133, Co-57 and Cd-109). The delay-line detectors can therefore be used to extract the charge information from both ion and gamma-ray interactions. Furthermore, standard charge-sensitive circuits yield high SNR

  9. Experimental studies on coherent synchrotron radiation at an emittance exchange beam line

    Science.gov (United States)

    Thangaraj, J. C. T.; Thurman-Keup, R.; Ruan, J.; Johnson, A. S.; Lumpkin, A. H.; Santucci, J.

    2012-11-01

    One of the goals of the Fermilab A0 photoinjector is to investigate experimentally the transverse to longitudinal emittance exchange (EEX) principle. Coherent synchrotron radiation in the emittance exchange line could limit the performance of the emittance exchanger at short bunch lengths. In this paper, we present experimental and simulation studies of the coherent synchrotron radiation (CSR) in the emittance exchange line at the A0 photoinjector. We report on time-resolved CSR studies using a skew-quadrupole technique. We also demonstrate the advantages of running the EEX with an energy-chirped beam.

  10. Construction of radiation-reduced hybrids and their use in mapping of microclones from chromosome 10p11.2-q11.2

    International Nuclear Information System (INIS)

    Fujita, Shoichi; Shin, Eisei; Nakamura, Tsutomu; Kurahashi, Hiroki; Mori, Takesada; Takai, Shin-ichiro; Nishisho, Isamu; Kaneda, Yasufumi; Tanaka, Kiyoji.

    1993-01-01

    Radiation-reduced hybrids for mapping of DNA markers in the pericentromeric region of chromosome 10 were developed. A Chinese hamster/human somatic cell hybrid (762-8A) carrying chromosomes 10 and Y as the only human material were exposed to 40,000 rads of irradiation and then rescued by fusion with non-irradiated recipient Chinese hamster cells (GM459). Southern hybridization analyses revealed that 10 of 128 HAT-resistant clones contained human chromosomal fragments corresponding to at least one marker locus between FNRB (10p11.2) and RBP3 (10q11.2). These hybrids were then used to map microdissection clones previously isolated and roughly mapped to this chromosomal region by fluorescence in situ hybridization (FISH). Two of the six microclones studies could be mapped to the proximity of the D10S102 locus. These radiation hybrids are useful for the construction of refined genetic maps of the pericentromeric region of chromosome 10. (author) 50 refs

  11. Hybrid Perovskites for Photovoltaics: Charge-Carrier Recombination, Diffusion, and Radiative Efficiencies.

    Science.gov (United States)

    Johnston, Michael B; Herz, Laura M

    2016-01-19

    values extracted from OPTP measurements and their dependence on perovskite composition and morphology. The significance of the reviewed charge-carrier recombination and mobility parameters is subsequently evaluated in terms of the charge-carrier diffusion lengths and radiative efficiencies that may be obtained for such hybrid perovskites. We particularly focus on calculating such quantities in the limit of ultra-low trap-related recombination, which has not yet been demonstrated but could be reached through further advances in material processing. We find that for thin films of hybrid lead iodide perovskites with typical charge-carrier mobilities of ∼30cm(2)/(V s), charge-carrier diffusion lengths at solar (AM1.5) irradiation are unlikely to exceed ∼10 μm even if all trap-related recombination is eliminated. We further examine the radiative efficiency for hybrid lead halide perovskite films and show that if high efficiencies are to be obtained for intermediate charge-carrier densities (n ≈ 10(14) cm(-3)) trap-related recombination lifetimes will have to be enhanced well into the microsecond range.

  12. Use of lymphoblastoid cell lines to evaluate the hypersensitivity to ultraviolet radiation in Cockayne syndrome

    International Nuclear Information System (INIS)

    Otsuka, F.; Tarone, R.E.; Cayeux, S.; Robbins, J.H.

    1984-01-01

    Cockayne syndrome (CS) is a rare autosomal recessive disease characterized by acute sun sensitivity, cachectic dwarfism, and neurologic and skeletal abnormalities. Cultured skin fibroblasts from patients with this disease are known to be hypersensitive to the lethal effects of 254-nm UV radiation. The authors have studied the sensitivity of 254-nm UV radiation of lymphoblastoid lines derived from 3 typical CS patients, 1 atypical CS patient who had a very late age of onset of clinical manifestations, 2 patients who had both xeroderma pigmentosum (XP) and typical CS, and 3 heterozygous parents of these patients. Post-UV survival was determined by the trypan-blue dye-exclusion method. The lymphoblastoid lines from the 3 typical CS patients, the atypical CS patient, and the 2 patients with both CS and XP had decreased post-UV viability in comparison with lines from normal donors. Lines from the heterozygous parents had normal post-UV viability. The post-UV viability of the typical CS lines was similar to that of a XP complementation group C line. The relative post-UV viability of lymphoblastoid lines from the typical CS patients was similar to the relative post-UV survival of their fibroblast lines. The lymphoblastoid line from the atypical CS patient had a post-UV viability similar to that of the typical CS patients. Thus, the relative hypersensitivity of CS patients cells in vitro does not reflect the severity or age of onset of the patients clinical manifestations. The lymphoblastoid lines from the 2 patients who had both CS and XP were significantly more sensitive to the UV radiation than those from patients with only CS. Our studies demonstrate that lymphoblastoid lines from patients with CS are appropriate and useful cell lines for the study of the inherited hypersensitivity to UV radiation

  13. Applications of the energy differentiation type radiation line sensor to such as inspection for the plumbing corrosion

    International Nuclear Information System (INIS)

    Tomita, Yasuhiro; Shirayanagi, Yuji; Matsui, Shinjiro; Kamiya, Yosuke; Kobayashi, Akira

    2015-01-01

    The authors have engaged in development of the next-generation radiation detectors that can give information on energy such as X-rays and γ-rays, and commercialized an energy discrimination type radiation line sensor capable of discriminating the energy of photons using a CdTe radiation detector element. This paper introduces the structure and principles of this energy discrimination type radiation line sensor. As the basic application, it also introduces the material identification, energy discrimination type X-ray CT imaging, and the quantitative determination of iron plate thickness using X-ray. In addition, it introduces the radiation line sensor we have developed for inspecting the reduced amount of wall thickness of piping with insulation materials. This radiation line sensor for pipe thinning inspection combined with radiation sources (X-rays, γ-rays) and a transport system is capable of accurately and efficiently inspecting reduced wall thickness, while moving the censor on the covered pipe with heat insulating materials through remote control, without removing piping insulation materials and without stopping the use of piping. (A.O.)

  14. Importance of representing optical depth variability for estimates of global line-shaped contrail radiative forcing.

    Science.gov (United States)

    Kärcher, Bernd; Burkhardt, Ulrike; Ponater, Michael; Frömming, Christine

    2010-11-09

    Estimates of the global radiative forcing by line-shaped contrails differ mainly due to the large uncertainty in contrail optical depth. Most contrails are optically thin so that their radiative forcing is roughly proportional to their optical depth and increases with contrail coverage. In recent assessments, the best estimate of mean contrail radiative forcing was significantly reduced, because global climate model simulations pointed at lower optical depth values than earlier studies. We revise these estimates by comparing the probability distribution of contrail optical depth diagnosed with a climate model with the distribution derived from a microphysical, cloud-scale model constrained by satellite observations over the United States. By assuming that the optical depth distribution from the cloud model is more realistic than that from the climate model, and by taking the difference between the observed and simulated optical depth over the United States as globally representative, we quantify uncertainties in the climate model's diagnostic contrail parameterization. Revising the climate model results accordingly increases the global mean radiative forcing estimate for line-shaped contrails by a factor of 3.3, from 3.5 mW/m(2) to 11.6 mW/m(2) for the year 1992. Furthermore, the satellite observations and the cloud model point at higher global mean optical depth of detectable contrails than often assumed in radiative transfer (off-line) studies. Therefore, we correct estimates of contrail radiative forcing from off-line studies as well. We suggest that the global net radiative forcing of line-shaped persistent contrails is in the range 8-20 mW/m(2) for the air traffic in the year 2000.

  15. [Evaluation of Radiation Dose during Stent-graft Treatment Using a Hybrid Operating Room System].

    Science.gov (United States)

    Haga, Yoshihiro; Chida, Kouichi; Kaga, Yuji; Saitou, Kazuhisa; Arai, Takeshi; Suzuki, Shinichi; Iwaya, Yoshimi; Kumasaka, Eriko; Kataoka, Nozomi; Satou, Naoto; Abe, Mitsuya

    2015-12-01

    In recent years, aortic aneurysm treatment with stent graft grafting in the X-ray fluoroscopy is increasing. This is an endovascular therapy, because it is a treatment which includes the risk of radiation damage, having to deal with radiation damage, to know in advance is important. In this study, in order to grasp the trend of exposure stent graft implantation in a hybrid operating room (OR) system, focusing on clinical data (entrance skin dose and fluoroscopy time), was to count the total. In TEVAR and EVAR, fluoroscopy time became 13.40 ± 7.27 minutes, 23.67 ± 11.76 minutes, ESD became 0.87 ± 0.41 mGy, 1.11 ± 0.57 mGy. (fluoroscopy time of EVAR was 2.0 times than TEVAR. DAP of EVAR was 1.2 times than TEVAR.) When using the device, adapted lesions and usage are different. This means that care changes in exposure-related factors. In this study, exposure trends of the stent graft implantation was able to grasp. It can be a helpful way to reduce/optimize the radiation dose in a hybrid OR system.

  16. Evaluation of radiation dose during sent-graft treatment using a hybrid operating room system

    International Nuclear Information System (INIS)

    Haga, Yoshihiro; Kaga, Yuji; Chida, Koichi

    2015-01-01

    In recent years, aortic aneurysm treatment with stent graft grafting in the X-ray fluoroscopy is increasing. This is an endovascular therapy, because it is a treatment which includes the risk of radiation damage, having to deal with radiation damage, to know in advance is important. In this study, in order to grasp the trend of exposure stent graft implantation in a hybrid operating room (OR) system, focusing on clinical data (entrance skin dose and fluoroscopy time), was to count the total. In TEVAR and EVAR, fluoroscopy time became 13.40 ± 7.27 minutes, 23.67 ± 11.76 minutes, ESD became 0.87 ± 0.41 mGy, 1.11 ± 0.57 mGy. (fluoroscopy time of EVAR was 2.0 times than TEVAR. DAP of EVAR was 1.2 times than TEVAR). When using the device, adapted lesions and usage are different. This means that care changes in exposure-related factors. In this study, exposure trends of the stent graft implantation was able to grasp. It can be a helpful way to reduce/optimize the radiation dose in a hybrid OR system. (author)

  17. Flat tie-line power scheduling control of grid-connected hybrid microgrids

    DEFF Research Database (Denmark)

    Xiao, Zhao xia; Guerrero, Josep M.; Shuang, Jia

    2018-01-01

    In future active distribution networks (ADNs), microgrids (MGs) may have the possibility to control the power dispatched to the ADN by coordinating the output power of their multiple renewable generation units and energy storage units (ESUs). In this way, each MG may support the active distribution....... Also, a method to calculate the tie-line power flow to be exchanged between the MG and the ADN is explored, and a power ramp rate is given between different dispatch intervals. Finally, a simulation model of the hybrid MG is built and tested. Simulation results show that the proposed hierarchical...

  18. Response of a hybrid pixel detector (MEDIPIX3) to different radiation sources for medical applications

    Energy Technology Data Exchange (ETDEWEB)

    Chumacero, E. Miguel; De Celis Alonso, B.; Martínez Hernández, M. I.; Vargas, G.; Moreno Barbosa, E., E-mail: emoreno.emb@gmail.com [Facultad de Ciencias Físico Matemáticas, Benemérita Universidad Autónoma de Puebla, Av. San Claudio y Rio Verde, Puebla (Mexico); Moreno Barbosa, F. [Hospital General del Sur Hospital de la Mujer, Puebla (Mexico)

    2014-11-07

    The development in semiconductor CMOS technology has enabled the creation of sensitive detectors for a wide range of ionizing radiation. These devices are suitable for photon counting and can be used in imaging and tomography X-ray diagnostics. The Medipix[1] radiation detection system is a hybrid silicon pixel chip developed for particle tracking applications in High Energy Physics. Its exceptional features (high spatial and energy resolution, embedded ultra fast readout, different operation modes, etc.) make the Medipix an attractive device for applications in medical imaging. In this work the energy characterization of a third-generation Medipix chip (Medipix3) coupled to a silicon sensor is presented. We used different radiation sources (strontium 90, iron 55 and americium 241) to obtain the response curve of the hybrid detector as a function of energy. We also studied the contrast of the Medipix as a measure of pixel noise. Finally we studied the response to fluorescence X rays from different target materials (In, Pd and Cd) for the two data acquisition modes of the chip; single pixel mode and charge summing mode.

  19. Human hybrid hybridoma

    Energy Technology Data Exchange (ETDEWEB)

    Tiebout, R.F.; van Boxtel-Oosterhof, F.; Stricker, E.A.M.; Zeijlemaker, W.P.

    1987-11-15

    Hybrid hybridomas are obtained by fusion of two cells, each producing its own antibody. Several authors have reported the construction of murine hybrid hybridomas with the aim to obtain bispecific monoclonal antibodies. The authors have investigated, in a model system, the feasibility of constructing a human hybrid hybridoma. They fused two monoclonal cell lines: an ouabain-sensitive and azaserine/hypoxanthine-resistant Epstein-Barr virus-transformed human cell line that produces an IgG1kappa antibody directed against tetanus toxiod and an azaserine/hypoxanthine-sensitive and ouabain-resistant human-mouse xenohybrid cell line that produces a human IgG1lambda antibody directed against hepatitis-B surface antigen. Hybrid hybridoma cells were selected in culture medium containing azaserine/hypoxanthine and ouabain. The hybrid nature of the secreted antibodies was analyzed by means of two antigen-specific immunoassay. The results show that it is possible, with the combined use of transformation and xenohybridization techniques, to construct human hybrid hybridomas that produce bispecific antibodies. Bispecific antibodies activity was measured by means of two radioimmunoassays.

  20. Improvement in operational characteristics of KEPCO’s line-commutation-type superconducting hybrid fault current limiter

    Science.gov (United States)

    Yim, S.-W.; Park, B.-C.; Jeong, Y.-T.; Kim, Y.-J.; Yang, S.-E.; Kim, W.-S.; Kim, H.-R.; Du, H.-I.

    2013-01-01

    A 22.9 kV class hybrid fault current limiter (FCL) developed by Korea Electric Power Corporation and LS Industrial Systems in 2006 operates using the line commutation mechanism and begins to limit the fault current after the first half-cycle. The first peak of the fault current is available for protective coordination in the power system. However, it also produces a large electromagnetic force and imposes a huge stress on power facilities such as the main transformer and gas-insulated switchgear. In this study, we improved the operational characteristics of the hybrid FCL in order to reduce the first peak of the fault current. While maintaining the structure of the hybrid FCL system, we developed a superconducting module that detects and limits the fault current during the first half-cycle. To maintain the protective coordination capacity, the hybrid FCL was designed to reduce the first peak value of the fault current by up to approximately 30%. The superconducting module was also designed to produce a minimum AC loss, generating a small, uniform magnetic field distribution during normal operation. Performance tests confirmed that when applied to the hybrid FCL, the superconducting module showed successful current limiting operation without any damage.

  1. Experimental studies on coherent synchrotron radiation at an emittance exchange beam line

    Directory of Open Access Journals (Sweden)

    J. C. T. Thangaraj

    2012-11-01

    Full Text Available One of the goals of the Fermilab A0 photoinjector is to investigate experimentally the transverse to longitudinal emittance exchange (EEX principle. Coherent synchrotron radiation in the emittance exchange line could limit the performance of the emittance exchanger at short bunch lengths. In this paper, we present experimental and simulation studies of the coherent synchrotron radiation (CSR in the emittance exchange line at the A0 photoinjector. We report on time-resolved CSR studies using a skew-quadrupole technique. We also demonstrate the advantages of running the EEX with an energy-chirped beam.

  2. Chromosomal Aberrations in DNA Repair Defective Cell Lines: Comparisons of Dose Rate and Radiation Quality

    Science.gov (United States)

    George, K. A.; Hada, M.; Patel, Z.; Huff, J.; Pluth, J. M.; Cucinotta, F. A.

    2009-01-01

    Chromosome aberration yields were assessed in DNA double-strand break repair (DSB) deficient cells after acute doses of gamma-rays or high-LET iron nuclei, or low dose-rate (0.018 Gy/hr) gamma-rays. We studied several cell lines including fibroblasts deficient in ATM (product of the gene that is mutated in ataxia telangiectasia patients) or NBS (product of the gene mutated in the Nijmegen breakage syndrome), and gliomablastoma cells that are proficient or lacking in DNA-dependent protein kinase, DNA-PK activity. Chromosomes were analyzed using the fluorescence in-situ hybridization (FISH) chromosome painting method in cells at the first division post-irradiation and chromosome aberrations were identified as either simple exchanges (translocations and dicentrics) or complex exchanges (involving >2 breaks in 2 or more chromosomes). Gamma radiation induced higher yields of both simple and complex exchanges in the DSB repair defective cells than in the normal cells. The quadratic dose-response terms for both chromosome exchange types were significantly higher for the ATM and NBS defective lines than for normal fibroblasts. However, the linear dose-response term was significantly higher only for simple exchanges in the NBS cells. Large increases in the quadratic dose response terms indicate the important roles of ATM and NBS in chromatin modifications that facilitate correct DSB repair and minimize aberration formation. Differences in the response of AT and NBS deficient cells at lower doses suggests important questions about the applicability of observations of radiation sensitivity at high dose to low dose exposures. For all iron nuclei irradiated cells, regression models preferred purely linear and quadratic dose responses for simple and complex exchanges, respectively. All the DNA repair defective cell lines had lower Relative biological effectiveness (RBE) values than normal cells, the lowest being for the DNA-PK-deficient cells, which was near unity. To further

  3. Nitrogen-15 uptake by whole plants and root callus cultures of inbred maize lines and their F1 hybrids

    International Nuclear Information System (INIS)

    Mladenova, Y.; Karadimova, M.

    1981-01-01

    The uptake of nitrogen-15 by 3 maize genotypes was investigated. Comparative analysis of N15 assimilation and distribution in the organs of intact plants of two self-pollinated lines and their F1 hybrid and also in a callus tissue of roots of the same genotypes was made. From the results the conclusion is drawn that the N-use efficiency of the female line is higher than that of the male line both in intact plants and callus tissues from roots. This fact indicates that the N-use efficiency is determined not only by the functions of the cells in the shoots, suggesting the participation of the photosynthetic carboxylases but also by the functions of cells without a photosynthesizing apparatus. The N-use efficiency in the F1 hybrid manifests ''heterosis'', in spite of the intact plants or root callus tissues are being studied. (author)

  4. Off-line NDA measurement of actinides in reprocessing solution using hybrid K-edge/K-XRF densitometer

    International Nuclear Information System (INIS)

    Bootharajan, M.; Swaminathan, K.; Venkata Subramani, C.R.; Kumar, R.

    2015-01-01

    A versatile, nondestructive assay (NDA) system of a hybrid K-edge/K-XRF facility adapted to a glove box facility has been developed at RCL, IGCAR for the analysis of U and Pu in process solutions obtained from the reprocessing of spent nuclear fuels. This paper describes i) The development of a hybrid K-edge/K-XRF facility adapted to a glove box system ii) The results obtained using conditioner solution of burn up 155 GWd/t with a dose of 20 R/h and iii) Comparison of the results with the parallel analyses of the same by Isotope dilution mass spectrometry. The hybrid K-edge cum K-XRF densitometer is ideally suited for dissolver solutions as well as U and Pu product solutions from reprocessing plant. This method can be useful in the analysis of mixed solution of Special Nuclear Materials (SNM) without chemical separation. To assay solutions with high radiation background, the hybrid K-edge/K-XRF system is designed and fabricated inside a glove box with adequate shielding from both source X-rays and the sample radiation. The theory and preliminary experiments are described elsewhere. Around 5 mL of the conditioner solution (burn up of 155 GWd/t with a dose of 20 R/h) was taken in a poly propylene vial placed concentrically in to another poly propylene vial. The concentration was estimated by K-edge densitometry with X-ray tube operated with 150 kV and 1 mA and counting period of 3000s. Background correction was obtained with the X-ray tube in OFF condition. The solution was analysed parallelly using isotopic dilution mass spectrometry

  5. Radiation of different human melanoma cell lines increased expression of RHOB. Level of this tumor suppressor gene in different cell lines

    International Nuclear Information System (INIS)

    Notcovich, C.; Molinari, B.; Duran, H.; Delgado González, D.; Sánchez Crespo, R.

    2013-01-01

    Previous results of our group show that a correlation exists between intrinsic radiosensitivity of human melanoma cells and cell death by apoptosis. RhoB is a small GTPase that regulates cytoskeletal organization. Besides, is related to the process of apoptosis in cells exposed to DNA damage as radiation. Also, RhoB levels decrease in a wide variety of tumors with the tumor stage, being considered a tumor suppressor gene due to its antiproliferative and proapoptotic effect. The aim of this study was to analyze the expression of RhoB in different human melanoma cell lines in relation to melanocytes, and evaluate the effect of gamma radiation on the expression of RhoB. We used the A375, SB2 and Meljcell lines, and the derived from melanocytes Pig1. It was found for all three tumor lines RhoB expression levels significantly lower than those of Pig1 (p <0.05), as assessed by semiquantitative RT-PCR . When tumor cells were irradiated to a dose of 2Gyinduction was observed at 3 hours RhoB irradiation. RhoB expression increased in all lines relative to non-irradiated control, showing a greater induction ( p< 0.05) for the more radiosensitive line SB2, consistent with apoptosis in response to radiation. The results allow for the first time in melanoma demonstrate that RhoB, as well as in other tumor types, has a lower expression in tumor cells than their normal counterparts. Moreover, induction in the expression of RhoB in irradiated cells may be associated with the process of radiation-induced apoptosis. The modulation of RhoB could be a new tool to sensitize radioresistant melanoma. (author)

  6. A hybrid method based on a new clustering technique and multilayer perceptron neural networks for hourly solar radiation forecasting

    International Nuclear Information System (INIS)

    Azimi, R.; Ghayekhloo, M.; Ghofrani, M.

    2016-01-01

    Highlights: • A novel clustering approach is proposed based on the data transformation approach. • A novel cluster selection method based on correlation analysis is presented. • The proposed hybrid clustering approach leads to deep learning for MLPNN. • A hybrid forecasting method is developed to predict solar radiations. • The evaluation results show superior performance of the proposed forecasting model. - Abstract: Accurate forecasting of renewable energy sources plays a key role in their integration into the grid. This paper proposes a hybrid solar irradiance forecasting framework using a Transformation based K-means algorithm, named TB K-means, to increase the forecast accuracy. The proposed clustering method is a combination of a new initialization technique, K-means algorithm and a new gradual data transformation approach. Unlike the other K-means based clustering methods which are not capable of providing a fixed and definitive answer due to the selection of different cluster centroids for each run, the proposed clustering provides constant results for different runs of the algorithm. The proposed clustering is combined with a time-series analysis, a novel cluster selection algorithm and a multilayer perceptron neural network (MLPNN) to develop the hybrid solar radiation forecasting method for different time horizons (1 h ahead, 2 h ahead, …, 48 h ahead). The performance of the proposed TB K-means clustering is evaluated using several different datasets and compared with different variants of K-means algorithm. Solar datasets with different solar radiation characteristics are also used to determine the accuracy and processing speed of the developed forecasting method with the proposed TB K-means and other clustering techniques. The results of direct comparison with other well-established forecasting models demonstrate the superior performance of the proposed hybrid forecasting method. Furthermore, a comparative analysis with the benchmark solar

  7. Origin, radiation, dispersion and allopatric hybridization in the chub Leuciscus cephalus.

    OpenAIRE

    Durand, J D; Unlü, E; Doadrio, I; Pipoyan, S; Templeton, A R

    2000-01-01

    The phylogenetic relationships of 492 chub (Leuciscus cephalus) belonging to 89 populations across the species' range were assessed using 600 base pairs of cytochrome b. Furthermore, nine species belonging to the L. cephalus complex were also analysed (over the whole cytochrome b) in order to test potential allopatric hybridization with L. cephalus sensu stricto (i.e. the chub). Our results show that the chub includes four highly divergent lineages descending from a quick radiation that took ...

  8. Radiation source states on-line supervision system design and implementation based on RFID technology

    International Nuclear Information System (INIS)

    Yang Binhua; Ling Qiu; Yin Guoli; Yang Kun; Wan Xueping; Wang Kan

    2011-01-01

    It puts forward radiation source states on-line monitoring resolution based on RFID technology. Firstly, the system uses RFID in real-time transmission of the radiation dose rate, and monitors the radiation source states and dose rate of the surrounding environment on-line. Then it adopts regional wireless networking mode to construct enterprise level monitoring network, which resolves long-distance wiring problems. And then it uses GPRS wireless to transport the real-time data to the monitoring center and the government supervision department, By adopting randomly dynamic cording in display update every day, it strengthens the supervision of the radiation source. At last this system has been successful applied to a thickness gauge project, which verifies the feasibility and practicality is good. (authors)

  9. Structure, shape, and evolution of radiatively accelerated QSO emission-line clouds

    International Nuclear Information System (INIS)

    Blumenthal, G.R.; Mathews, W.G.

    1979-01-01

    The possibility that the broad emission-line regions of QSOs and active galactic nuclei are formed by a multitude of small clouds which are radiatively accelerated is discussed. Although this model is by no means certain at present, it has four virtues: (1) Observed emission-line widths can be produced with observationally allowed electron densities, UV luminosities, and ionization levels. (2) The acceleration force is coherent in each cloud are found. (3) Reasonable line profiles can result for all emission lines. (4) Photoionization of hydrogen accounts for both heating and acceleration of the emission-line gas. A self-consistent model is developed for the structure, shape, and evolution of radiatively accelerated clouds. The shape varies with cloud mass, and two distinct types of clouds. Fully ionized clouds of very low mass approach a nearly spherical shape. However, all clouds having masses greater than some critical mass adopt a ''pancake'' shape. The condition for constant cloud mass in the cloud frame is shown to be equivalent to the equation of motion of a cloud in the rest frame of the QSO. The emission-line profiles can be sensitive to radial variations in the properties of the intercloud medium, and those properties that correspond to observed profiles are discussed. Finally, the covering factor of a system of pancake clouds is estimated along with the total number of clouds required--approximately 10 14 clouds in each QSO

  10. Radiation-induced alterations of histone post-translational modification levels in lymphoblastoid cell lines

    International Nuclear Information System (INIS)

    Maroschik, Belinda; Gürtler, Anne; Krämer, Anne; Rößler, Ute; Gomolka, Maria; Hornhardt, Sabine; Mörtl, Simone; Friedl, Anna A

    2014-01-01

    Radiation-induced alterations in posttranslational histone modifications (PTMs) may affect the cellular response to radiation damage in the DNA. If not reverted appropriately, altered PTM patterns may cause long-term alterations in gene expression regulation and thus lead to cancer. It is therefore important to characterize radiation-induced alterations in PTM patterns and the factors affecting them. A lymphoblastoid cell line established from a normal donor was used to screen for alterations in methylation levels at H3K4, H3K9, H3K27, and H4K20, as well as acetylation at H3K9, H3K56, H4K5, and H4K16, by quantitative Western Blot analysis at 15 min, 1 h and 24 h after irradiation with 2 Gy and 10 Gy. The variability of alterations in acetylation marks was in addition investigated in a panel of lymphoblastoid cell lines with differing radiosensitivity established from lung cancer patients. The screening procedure demonstrated consistent hypomethylation at H3K4me3 and hypoacetylation at all acetylation marks tested. In the panel of lymphoblastoid cell lines, however, a high degree of inter-individual variability became apparent. Radiosensitive cell lines showed more pronounced and longer lasting H4K16 hypoacetylation than radioresistant lines, which correlates with higher levels of residual γ-H2AX foci after 24 h. So far, the factors affecting extent and duration of radiation-induced histone alterations are poorly defined. The present work hints at a high degree of inter-individual variability and a potential correlation of DNA damage repair capacity and alterations in PTM levels

  11. Generalized transmission line method to study the far-zone radiation of antennas under a multilayer structure

    CERN Document Server

    Wu, Xuan Hui

    2008-01-01

    This book gives a step-by-step presentation of a generalized transmission line method to study the far-zone radiation of antennas under a multilayer structure. Normally, a radiation problem requires a full wave analysis which may be time consuming. The beauty of the generalized transmission line method is that it transforms the radiation problem for a specific type of structure, say the multilayer structure excited by an antenna, into a circuit problem that can be efficiently analyzed. Using the Reciprocity Theorem and far-field approximation, the method computes the far-zone radiation due to

  12. GARLIC, a shielding program for GAmma Radiation from Line- and Cylinder-sources

    Energy Technology Data Exchange (ETDEWEB)

    Roos, Matts

    1959-07-15

    GARLIC is a program for computing the gamma ray flux or dose rate at a shielded idotropic point detector, due to a line source or the line equivalent of a cylindrical source. The source strength distribution along the line must be either uniform or an arbitrary part of the positive half-cycle of a cosine function. The line source can be oriented arbitrarily with respect to the main shield and the detector, except that the detector must not be located on the line source or on its extension. The main source is a homogeneous plane slab in which scattered radiation is accounted for by multiplying each point element of the line source by a point source build-up factor inside the integral over the point elements. Between, the main shield and the line source additional shields can be introduced, which are either plane slabs, parallel to the main shield, or cylindrical rings, coaxial with the line source. Scattered radiation in the additional shields can only be accounted for by constant build-up factors outside the integral. GARLIC-xyz is an extended version particularly suited for the frequently met problem of shielding a room containing a large number of line sources in different positions. The program computes the angles and linear dimensions of a problem for GARLIC when the positions of the detector point and the end points of the line source are given as points in an arbitrary rectangular coordinate system. As an example the isodose curves in water are presented for a monoenergetic cosine-distributed line source at several source energies and for an operating fuel element of the Swedish reactor R3.

  13. Assesment of spineless safflower (Carthamus tinctorius, L.) mutant lines for seed oil content and fatty acid profiles

    International Nuclear Information System (INIS)

    Ragab, A.I.; Kassem, M.; Moustafa, H.A.M.

    2008-01-01

    This study was conducted to assess the new spineless mutants that previously induced through gamma radiation and hybridization techniques in the advanced generation for seed oil content and fatty acid profiles The obtained results cleared that oil percentages of all seven safflower mutants were increased than local variety Giza (1) and the new mutant hybrid 2 line (white petals) had the highest increase value of oil percentage (10%) but the mutant line M14 (dark red petals) had the lowest increase value of oil percentage (3.1 %) The mutant line M7 (yellow petals) had the highest value of total saturated fatty acid (40.38%), because it had the highest value of palmitic fatty acid (25.16%), comparing to 10.01% value for local variety Giza (1), followed by mutant line hybrid 2 (white petals) which had (39.88%) because it had the highest value of caprylic, capric, lauric, myristic and stearic fatty acids. All safflower mutant lines had higher value of oleic fatty acid than that of the local variety Giza (1) the two new safflower mutant lines M7 (yellow petals) and hybrid 2 (white petal) had the highest value of oleic fatty acid 41.22% and 39.88% respectively in comparison with 13.5% for local variety Giza (1), the obtained results are indicating to seed oil content negative correlation between oleic and linoleic acids

  14. Chromosomal instability induced by ionizing radiation

    International Nuclear Information System (INIS)

    Morgan, W.F.; Marder, B.A.; Day, J.P.

    1995-01-01

    There is accumulating evidence indicating genomic instability can manifest multiple generations after cellular exposure to DNA damaging agents. For instance, some cells surviving exposure to ionizing radiations show delayed reproductive cell death, delayed mutation and / or delayed chromosomal instability. Such instability, especially chromosome destabilization has been implicated in mutation, gene amplification, cellular transformation, and cell killing. To investigate chromosomal instability following DNA damage, we have used fluorescence in situ hybridization to detect chromosomal rearrangements in a human/hamster somatic hybrid cell line following exposure to ionizing radiation. Delayed chromosomal instability was detected when multiple populations of uniquely arranged metaphases were observed in clonal isolates raised from single cells. The relationship between delayed chromosomal destabilization and other endpoints of genomic instability, namely; delayed mutation and gene amplification will be discussed, as will the potential cytogenetic and molecular mechanisms contributing to delayed chromosomal instability

  15. DSP Control of Line Hybrid Active Filter

    DEFF Research Database (Denmark)

    Dan, Stan George; Benjamin, Doniga Daniel; Magureanu, R.

    2005-01-01

    Active Power Filters have been intensively explored in the past decade. Hybrid active filters inherit the efficiency of passive filters and the improved performance of active filters, and thus constitute a viable improved approach for harmonic compensation. In this paper a parallel hybrid filter...... is studied for current harmonic compensation. The hybrid filter is formed by a single tuned Le filter and a small-rated power active filter, which are directly connected in series without any matching transformer. Thus the required rating of the active filter is much smaller than a conventional standalone...... active filter. Simulation and experimental results obtained in laboratory confirmed the validity and effectiveness of the control....

  16. Origin, radiation, dispersion and allopatric hybridization in the chub Leuciscus cephalus.

    Science.gov (United States)

    Durand, J D; Unlü, E; Doadrio, I; Pipoyan, S; Templeton, A R

    2000-08-22

    The phylogenetic relationships of 492 chub (Leuciscus cephalus) belonging to 89 populations across the species' range were assessed using 600 base pairs of cytochrome b. Furthermore, nine species belonging to the L. cephalus complex were also analysed (over the whole cytochrome b) in order to test potential allopatric hybridization with L. cephalus sensu stricto (i.e. the chub). Our results show that the chub includes four highly divergent lineages descending from a quick radiation that took place three million years ago. The geographical distribution of these lineages and results of the nested clade analysis indicated that the chub may have originated from Mesopotamia. Chub radiation probably occurred during an important vicariant event such as the isolation of numerous Turkish river systems, a consequence of the uplift of the Anatolian Plateau (formerly covered by a broad inland lake). Dispersion of these lineages arose from the changes in the European hydrographic network and, thus, the chub and endemic species of the L. cephalus complex met by secondary contacts. Our results show several patterns of introgression, from Leuciscus lepidus fully introgressed by chub mitochondrial DNA to Leuciscus borysthenicus where no introgression at all was detected. We assume that these hybridization events might constitute an important evolutionary process for the settlement of the chub in new environments in the Mediterranean area.

  17. Expression of Ku correlates with radiation sensitivities in the head and neck cancer cell lines

    International Nuclear Information System (INIS)

    Lee, Sang Wook; Yu, Eun Sil; Yi, So Lyoung; Son, Se Hee; Kim, Jong Hoon; Ahn, Seung Do; Shin, Seong Soo; Choi, Eun Kyung

    2004-01-01

    DNA-dependent protein kinase (DNA-PK) is a serine/threonine kinase consisting of a 470 kDa catalytic subunit (DNA-PKcs) and a heterodimeric regulatory complex, called Ku, which is composed of 70 kDa (Ku 70) and 86 kDa (Ku 80) proteins. The DNA-PK has been shown to play a pivotal role in rejoining DNA double-strand-breaks (dsb) in mammalian cells. The purpose of this study is to examine the relationship between the level of Ku expression and radiation sensitivity. Nine head and neck, cancer cell lines showed various intrinsic radiation sensitivities. Among the nine, AMC-HN-3 cell was the most sensitive for X-ray irradiation and AMC-HN-9 cell was the most resistance. The most sensitive and resistant cell lines were selected and the test sensitivity of radiation and expression of Ku were measured. Radiation sensitivity was obtained by colony forming assay and Ku protein expression using Western blot analysis. Ku80 increased expression by radiation, wheras Ku70 did not. Overexpression of Ku80 protein increased radiation resistance in AMC-HN9 cell line. There was a correlation between Ku80 expression and radiation resistance. Ku80 was shown to play an important role in radiation damage response. Induction of Ku80 expression had an important role in DNA damage repair by radiation. Ku80 expression may be an effective predictive assay of radiosensitivity on head and neck cancer

  18. Hybrid radiation background monitoring in operational control and forecasting of environmental contamination by nuclear power station discharges

    International Nuclear Information System (INIS)

    Ermeev, I.S.; Eremenko, V.A.; Makarov, Y.A.; Matueev, V.V.; Zhernov, V.S.

    1986-01-01

    Rapid developments in nuclear power have stimulated research on monitoring and forecasting environmental radiation pollution (ERP), and in particular the amounts, compositions, and distributions of radionuclides in the environment. A conceptual model is presented for hybrid environmental radiation pollution monitoring. When there is an emergency, the model operates in a fashion most closely corresponding to the actual meteorological conditions, and the ERP data given by the model enable one to distinguish changes due to the man-made component from random fluctuations in the natural background. The measurement system in general includes mobile and stationary data-acquisition facilities linked by wire or radio to the central point. The system also accumulates and stores data on the radiation environment, which are edited on the basis of radioactive, chemical, and other transformations. The purpose of hybrid monitoring is ultimately to analyze trends in order to detect elevated discharges and thus to output data to the regional monitoring system

  19. Near-term hybrid vehicle program, phase 1. Appendix B: Design trade-off studies. [various hybrid/electric power train configurations and electrical and mechanical drive-line components

    Science.gov (United States)

    1979-01-01

    The relative attractiveness of various hybrid/electric power train configurations and electrical and mechanical drive-line components was studied. The initial screening was concerned primarily with total vehicle weight and economic factors and identified the hybrid power train combinations which warranted detailed evaluation over various driving cycles. This was done using a second-by-second vehicle simulation program which permitted the calculations of fuel economy, electricity usage, and emissions as a function of distance traveled in urban and highway driving. Power train arrangement possibilities were examined in terms of their effect on vehicle handling, safety, serviceability, and passenger comfort. A dc electric drive system utilizing a separately excited motor with field control and battery switching was selected for the near term hybrid vehicle. Hybrid vehicle simulations showed that for the first 30 mi (the electric range of the vehicle) in urban driving, the fuel economy was 80 mpg using a gasoline engine and 100 mpg using a diesel engine. In urban driving the hybrid would save about 75% of the fuel used by the conventional vehicle and in combined urban/highway driving the fuel saving is about 50%.

  20. Radiation sensitivity of Merkel cell carcinoma cell lines

    Energy Technology Data Exchange (ETDEWEB)

    Leonard, J.H.; Ramsay, J.R.; Birrell, G.W. [Queensland Institute of Medical Research (Australia)] [and others

    1995-07-30

    Merkel cell carcinoma (MCC), being a small cell carcinoma, would be expected to be sensitive to radiation. Clinical analysis of patients at our center, especially those with macroscopic disease, would suggest the response is quite variable. We have recently established a number of MCC cell lines from patients prior to radiotherapy, and for the first time are in a position to determine their sensitivity under controlled conditions. Some of the MCC lines grew as suspension cultures and could not be single cell cloned; therefore, it was not possible to use clonogenic survival for all cell lines. A tetrazolium based (MTT) assay was used for these lines, to estimate cell growth after {gamma} irradiation. Control experiments were conducted on lymphoblastoid cell lines (LCL) and the adherent MCC line, MCC13, to demonstrate that the two assays were comparable under the conditions used. We have examined cell lines from MCC, small cell lung cancer (SCLC), malignant melanomas, Epstein Barr virus (EBV) transformed lymphocytes (LCL), and skin fibroblasts for their sensitivity to {gamma} irradiation using both clonogenic cell survival and MTT assays. The results show that the tumor cell lines have a range of sensitivities, with melanoma being more resistant (surviving fraction at 2 Gy (SF2) 0.57 and 0.56) than the small cell carcinoma lines, MCC (SF2 range 0.21-0.45, mean SF2 0.30, n = 8) and SCLC (SF2 0.31). Fibroblasts were the most sensitive (SF2 0.13-0.20, mean 0.16, n = 5). The MTT assay, when compared to clonogenic assay for the MCC13 adherent line and the LCL, gave comparable results under the conditions used. Both assays gave a range of SF2 values for the MCC cell lines, suggesting that these cancers would give a heterogeneous response in vivo. The results with the two derivative clones of MCC14 (SF2 for MCC14/1 0.38, MCC14/2 0.45) would further suggest that some of them may develop resistance during clonogenic evolution. 25 refs., 3 figs., 1 tab.

  1. Radiation sensitivity of Merkell cell carcinoma cell lines

    International Nuclear Information System (INIS)

    Leonard, J. Helen; Ramsay, Jonathan R.; Kearsley, John H.; Birrell, Geoff W.

    1995-01-01

    Purpose: Merkel cell carcinoma (MCC), being a small cell carcinoma, would be expected to be sensitive to radiation. Clinical analysis of patients at our center, especially those with macroscopic disease, would suggest the response is quite variable. We have recently established a number of MCC cell lines from patients prior to radiotherapy, and for the first time are in a position to determine their sensitivity under controlled conditions. Methods and Materials: Some of the MCC lines grew as suspension cultures and could not be single cell cloned; therefore, it was not possible to use clonogenic survival for all cell lines. A tetrazolium based (MTT) assay was used for these lines, to estimate cell growth after γ irradiation. Control experiments were conducted on lymphoblastoid cell lines (LCL) and the adherent MCC line, MCC13, to demonstrate that the two assays were comparable under the conditions used. Results: We have examined cell lines from MCC, small cell lung cancer (SCLC), malignant melanomas, Epstein Barr virus (EBV) transformed lymphocytes (LCL), and skin fibroblasts for their sensitivity to γ irradiation using both clonogenic cell survival and MTT assays. The results show that the tumor cell lines have a range of sensitivities, with melanoma being more resistant (surviving fraction at 2 Gy (SF2) 0.57 and 0.56) than the small cell carcinoma lines, MCC (SF2 range 0.21-0.45, mean SF2 0.30, n = 8) and SCLC (SF2 0.31). Fibroblasts were the most sensitive (SF2 0.13-0.20, mean 0.16, n = 5). The MTT assay, when compared to clonogenic assay for the MCC13 adherent line and the LCL, gave comparable results under the conditions used. Conclusion: Both assays gave a range of SF2 values for the MCC cell lines, suggesting that these cancers would give a heterogeneous response in vivo. The results with the two derivative clones of MCC14 (SF2 for MCC14/1 0.38, MCC14/2 0.45) would further suggest that some of them may develop resistance during clonogenic evolution

  2. Radiating nonuniform transmission-line systems and the partial element equivalent circuit method

    CERN Document Server

    Nitsch, Juergen; Wollenberg, Gunter

    2009-01-01

    High frequencies of densely packed modern electronic equipment turn even the smallest piece of wire into a transmission line with signal retardation, dispersion, attenuation, and distortion. In electromagnetic environments with high-power microwave or ultra-wideband sources, transmission lines pick up noise currents generated by external electromagnetic fields. These are superimposed on essential signals, the lines acting not only as receiving antennas but radiating parts of the signal energy into the environment. This book is outstanding in its originality. While many textbooks rephrase

  3. DNA from radiation resistant human tumor cells transfers resistance to NIH/3T3 cells with varying degrees of penetrance

    International Nuclear Information System (INIS)

    Kasid, U.; Dritschilo, A.; Weichselbaum, R.

    1987-01-01

    Experimental evidence suggests that clinical radiation resistance may correlate with in vitro radiation survival parameters. Specifically, they isolated several cell lines from radioresistant head and neck carcinomas with D/sub 0/ values greater than 2 Gy. The authors co-transfected DNA from cell line SQ2OB (D/sub 0/ = 2.4 Gy) with the rhoSVNeO plasmid into NIH/3T3 cells (D/sub 0/ = 1.7 Gy). Antibiotic G418 resistant, transformed clones were isolated and confirmed by Southern blotting to contain human alu, as well as rhoSVNeO sequences. Screening for radiation resistance with 8Gy (Cs-137) revealed that 3 of 4 tested hybrid clones show a radiation survival intermediate between NIH/3T3 and SQ2OB. This suggests that radiation resistance is a dominant, transfectable phenotype of mammalian cells and can be expressed in more sensitive cells. Karyotyping of resistant hybrid clones shows the presence of double minute chromosomes. Secondary transfection results and experiments to clone the genetic factors responsible for radiation resistance are in progress and results will be reported

  4. CFD analysis of heat transfer performance of graphene based hybrid nanofluid in radiators

    Science.gov (United States)

    Bharadwaj, Bharath R.; Sanketh Mogeraya, K.; Manjunath, D. M.; Rao Ponangi, Babu; Rajendra Prasad, K. S.; Krishna, V.

    2018-04-01

    For Improved performance of an automobile engine, Cooling systems are one of the critical systems that need attention. With increased capacity to carry away large amounts of wasted heat, performance of an engine is increased. Current research on Nano-fluids suggests that they offer higher heat transfer rate compared to that of conventional coolants. Hence this project seeks to investigate the use of hybrid-nanofluids in radiators so as to increase its heat transfer performance. Carboxyl Graphene and Graphene Oxide based nanoparticles were selected due to the very high thermal conductivity of Graphene. System Analysis of the radiator was performed by considering a small part of the whole automobile radiator modelled using SEIMENS NX. CFD analysis was conducted using ANSYS FLUENT® for the nanofluid defined and the increase in effectiveness was compared to that of conventional coolants. Usage of such nanofluids for a fixed cooling requirement in the future can lead to significant downsizing of the radiator.

  5. The influence of continuum radiation fields on hydrogen radio recombination lines

    Science.gov (United States)

    Prozesky, Andri; Smits, Derck P.

    2018-05-01

    Calculations of hydrogen departure coefficients using a model with the angular momentum quantum levels resolved that includes the effects of external radiation fields are presented. The stimulating processes are important at radio frequencies and can influence level populations. New numerical techniques with a solid mathematical basis have been incorporated into the model to ensure convergence of the solution. Our results differ from previous results by up to 20 per cent. A direct solver with a similar accuracy but more efficient than the iterative method is used to evaluate the influence of continuum radiation on the hydrogen population structure. The effects on departure coefficients of continuum radiation from dust, the cosmic microwave background, the stellar ionising radiation, and free-free radiation are quantified. Tables of emission and absorption coefficients for interpreting observed radio recombination lines are provided.

  6. Fingerprinting and genetic purity assessment of F1 barley hybrids and their salt-tolerant parental lines using nSSR molecular markers.

    Science.gov (United States)

    Ben Romdhane, Mériam; Riahi, Leila; Jardak, Rahma; Ghorbel, Abdelwahed; Zoghlami, Nejia

    2018-01-01

    Hybridity and the genuineness of hybrids are prominent characteristics for quality control of seeds and thereby for varietal improvement. In the current study, the cross between two local barley genotypes (Ardhaoui: female; Testour: male) previously identified as susceptible/tolerant to salt stress in Tunisia was achieved. The hybrid genetic purity of the generated F 1 putative hybrids and the fingerprinting of the parents along with their offspring were assessed using a set of 17 nuclear SSR markers. Among the analyzed loci, 11 nSSR were shown polymorphic among the parents and their offspring. Based on the applied 11 polymorphic SSR loci, a total of 28 alleles were detected with an average of 2.54 alleles per locus. The locus HVM33 presented the highest number of alleles. The highest polymorphism information content value was detected for the locus HVM33 (0.6713) whereas the lowest PIC value (0.368) was revealed by the loci BMAC0156 , EBMAC0970 and BMAG0013 with a mean value of 0.4619. The probabilities of identical genotypes PI for the 11 microsatellite markers were 8.63 × 10 -7 . Banding patterns among parents and hybrids showed polymorphic fragments. The 11 SSR loci had produced unique fingerprints for each analyzed genotype and segregate between the two parental lines and their four hybrids. Parentage analysis confirms the hybrid purity of the four analyzed genotypes. Six Tunisian barley accessions were used as an outgroup in the multivariate analysis to confirm the efficiency of the employed 11 nSSR markers in genetic differentiation among various barley germplasms. Thus, neighbor joining and factorial analysis revealed clearly the discrimination among the parental lines, the four hybrids and the outgroup accessions. Out of the detected polymorphic 11 nuclear SSR markers, a set of five markers ( HVM33 , WMC1E8 , BMAC0154 , BMAC0040 and BMAG0007 ) were shown to be sufficient and informative enough to discriminate among the six genotypes representing the two

  7. Blot hybridization analysis of TCR genes of T cells for five people exposed in a radiation accident

    International Nuclear Information System (INIS)

    Min Rui; Liu Benti; Cheng Tianmin; Yang Rujun; Meng Xiangshun; Xiao Jinsong

    1996-01-01

    Human lymphocyte total DNA was prepared in agarose plug by mixing cells with low melting agarose, and two restriction endonucleases were used for digestion of the total DNA with human α and β TCR cDNA probes. The total digested DNA from five people who were whole body exposed to 2.0-2.5 Gy ionizing radiation in an accident 4.5 years ago was hybridized by Southern blot method. The results showed that no obvious difference in hybridization bands was found between controls and the five victims when hybridizations were fulfilled in the total DNA which was digested by Hind III restriction endonuclease with both α and β probes. However, when the total DNA was digested with restriction endonuclease EcoR I and was hybridized with TCR α probe, four of the five exposed people showed a different hybridizing band pattern compared with the controls. The results are also discussed

  8. Novel CMS lines in pigeonpea [Cajanus cajan (L. Millspaugh] derived from cytoplasmic substitutions, and their effective restoration and deployment in hybrid breeding

    Directory of Open Access Journals (Sweden)

    Abhishek Bohra

    2017-02-01

    Full Text Available The availability of stable cytoplasmic male sterile (CMS or A lines coupled with a robust restoration system (R lines is an essential prerequisite for efficient hybrid breeding. CMS-enabled hybrid technology holds immense potential to enhance the long-stagnant productivity of pigeonpea. In the present investigation, cytoplasmic substitutions were made in the nuclear backgrounds of early-maturing pigeonpea varieties or lines. Three new CMS lines (ICPL 88039A, Pusa 992A, and DPP 3-2A resulted from genetic crosses involving cytoplasmic donors from A2 (GT 288A and A4 (ICPA 2089 categories. In addition to visual inspection of anthers, pollen-staining techniques and scanning electron microscopy (SEM analysis were used to confirm pollen sterility. Further, given the relevance of the plant mitochondrial genome to CMS manifestation, 25 mitochondrion-specific DNA markers were assayed on these newly developed A lines and isogenic maintainer (B lines. DNA polymorphism between Pusa 992A and Pusa 992B as revealed by the nad7a_del marker confirmed the successful combination of sterilizing cytoplasm (A4 and nonrestoring nuclear background (Pusa 992. Such cytoplasm-specific DNA markers are required for A2-CMS as well. Further, to assess restoration ability, potential restorers were crossed with these CMS lines, and as a consequence, promising A × R combinations exhibiting 100% pollen fertility could be identified. In parallel, we also analyzed the inheritance patterns underlying fertility restoration using ICPL 88039A-derived F2 and BC1F1 populations, and established a monogenic dominant model to explain the phenomenon of A2-CMS restoration. In summary, we report the successful development of new CMS lines and describe their effective deployment in hybrid breeding of pigeonpea.

  9. Hybrid hydrogels produced by ionizing radiation technique

    International Nuclear Information System (INIS)

    Oliveira, M.J.A.; Amato, V.S.; Lugão, A.B.; Parra, D.F.

    2012-01-01

    The interest in biocompatible hydrogels with particular properties has increased considerably in recent years due to their versatile applications in biomedicine, biotechnology, pharmacy, agriculture and controlled release of drugs. The use of hydrogels matrices for particular drug-release applications has been investigated with the synthesis of modified polymeric hydrogel of PVAl and 0.5, 1.0, 1.5% nano-clay. They were processed using gamma radiation from Cobalt-60 source at 25 kGy dose. The characterization of the hydrogels was conducted and toxicity was evaluated. The dried hydrogel was analyzed for thermogravimetry analysis (TGA), infrared spectroscopy (FTIR) and swelling in solutions of different pH. The membranes have no toxicity. The nano-clay influences directly the equilibrium swelling. - Highlights: ► Chemical interaction is observed when nanoclay is irradiated in PVAl hybrid hydrogels. ► Osmotic pressure within network promotes the rehydration capacity of the membranes. ► This effect is an important characteristic for hydrogels drug delivery systems.

  10. Calculation of the line shapes of radiators immersed in plasma

    International Nuclear Information System (INIS)

    Hayrapetian, A.S.

    1987-01-01

    This work reports the results of theoretical calculations of line shapes of radiators immersed in plasma. The fluctuating electric field of the plasma perturbs the atomic structure of the immersed ions or atoms. The perturbed line shape is an important diagnostic tool for the temperature and density measurements of plasma. The line-shape calculation here is done by first deriving the line-shape expression, then it is shown that the problem is equivalent to calculating the temperature Green's function of the bound electron. The total Hamiltonian of the system includes the atomic, plasma, and atom-plasma parts. The temperature Green's function is calculated perturbatively by expanding in orders of atom-plasma interaction. By solving a Dyson equation, the dressed Green's functions of the bound electrons are obtained. At this point, the line shape is calculated by an analytic continuation from the complex frequency plane to real line. To derive the atomic electron Green's function, the momentum integral in the self-energy is approximated by a Riemann sum. With this approximation, the algebraic form of the line shape is obtained for an undetermined number of terms in the Riemann sum. Numerical calculation of line shape is done by using this result

  11. Planning Hybrid Intensity Modulated Radiation Therapy for Whole-breast Irradiation

    Energy Technology Data Exchange (ETDEWEB)

    Farace, Paolo [Medical Physics Department, Regional Oncological Hospital, Cagliari (Italy); Zucca, Sergio; Solla, Ignazio; Fadda, Giuseppina; Durzu, Silvia; Porru, Sergio; Meleddu, Gianfranco [Medical Physics Department, Regional Oncological Hospital, Cagliari (Italy); Deidda, Maria Assunta; Possanzini, Marco; Orru, Sivia; Lay, Giancarlo [Radiotherapy Department, Regional Oncological Hospital, Cagliari (Italy)

    2012-09-01

    Purpose: To test tangential and not-tangential hybrid intensity modulated radiation therapy (IMRT) for whole-breast irradiation. Methods and Materials: Seventy-eight (36 right-, 42 left-) breast patients were randomly selected. Hybrid IMRT was performed by direct aperture optimization. A semiautomated method for planning hybrid IMRT was implemented using Pinnacle scripts. A plan optimization volume (POV), defined as the portion of the planning target volume covered by the open beams, was used as the target objective during inverse planning. Treatment goals were to prescribe a minimum dose of 47.5 Gy to greater than 90% of the POV and to minimize the POV and/or normal tissue receiving a dose greater than 107%. When treatment goals were not achieved by using a 4-field technique (2 conventional open plus 2 IMRT tangents), a 6-field technique was applied, adding 2 non tangential (anterior-oblique) IMRT beams. Results: Using scripts, manual procedures were minimized (choice of optimal beam angle, setting monitor units for open tangentials, and POV definition). Treatment goals were achieved by using the 4-field technique in 61 of 78 (78%) patients. The 6-field technique was applied in the remaining 17 of 78 (22%) patients, allowing for significantly better achievement of goals, at the expense of an increase of low-dose ({approx}5 Gy) distribution in the contralateral tissue, heart, and lungs but with no significant increase of higher doses ({approx}20 Gy) in heart and lungs. The mean monitor unit contribution to IMRT beams was significantly greater (18.7% vs 9.9%) in the group of patients who required 6-field procedure. Conclusions: Because hybrid IMRT can be performed semiautomatically, it can be planned for a large number of patients with little impact on human or departmental resources, promoting it as the standard practice for whole-breast irradiation.

  12. Planning hybrid intensity modulated radiation therapy for whole-breast irradiation.

    Science.gov (United States)

    Farace, Paolo; Zucca, Sergio; Solla, Ignazio; Fadda, Giuseppina; Durzu, Silvia; Porru, Sergio; Meleddu, Gianfranco; Deidda, Maria Assunta; Possanzini, Marco; Orrù, Sivia; Lay, Giancarlo

    2012-09-01

    To test tangential and not-tangential hybrid intensity modulated radiation therapy (IMRT) for whole-breast irradiation. Seventy-eight (36 right-, 42 left-) breast patients were randomly selected. Hybrid IMRT was performed by direct aperture optimization. A semiautomated method for planning hybrid IMRT was implemented using Pinnacle scripts. A plan optimization volume (POV), defined as the portion of the planning target volume covered by the open beams, was used as the target objective during inverse planning. Treatment goals were to prescribe a minimum dose of 47.5 Gy to greater than 90% of the POV and to minimize the POV and/or normal tissue receiving a dose greater than 107%. When treatment goals were not achieved by using a 4-field technique (2 conventional open plus 2 IMRT tangents), a 6-field technique was applied, adding 2 non tangential (anterior-oblique) IMRT beams. Using scripts, manual procedures were minimized (choice of optimal beam angle, setting monitor units for open tangentials, and POV definition). Treatment goals were achieved by using the 4-field technique in 61 of 78 (78%) patients. The 6-field technique was applied in the remaining 17 of 78 (22%) patients, allowing for significantly better achievement of goals, at the expense of an increase of low-dose (∼5 Gy) distribution in the contralateral tissue, heart, and lungs but with no significant increase of higher doses (∼20 Gy) in heart and lungs. The mean monitor unit contribution to IMRT beams was significantly greater (18.7% vs 9.9%) in the group of patients who required 6-field procedure. Because hybrid IMRT can be performed semiautomatically, it can be planned for a large number of patients with little impact on human or departmental resources, promoting it as the standard practice for whole-breast irradiation. Copyright © 2012 Elsevier Inc. All rights reserved.

  13. Planning Hybrid Intensity Modulated Radiation Therapy for Whole-breast Irradiation

    International Nuclear Information System (INIS)

    Farace, Paolo; Zucca, Sergio; Solla, Ignazio; Fadda, Giuseppina; Durzu, Silvia; Porru, Sergio; Meleddu, Gianfranco; Deidda, Maria Assunta; Possanzini, Marco; Orrù, Sivia; Lay, Giancarlo

    2012-01-01

    Purpose: To test tangential and not-tangential hybrid intensity modulated radiation therapy (IMRT) for whole-breast irradiation. Methods and Materials: Seventy-eight (36 right-, 42 left-) breast patients were randomly selected. Hybrid IMRT was performed by direct aperture optimization. A semiautomated method for planning hybrid IMRT was implemented using Pinnacle scripts. A plan optimization volume (POV), defined as the portion of the planning target volume covered by the open beams, was used as the target objective during inverse planning. Treatment goals were to prescribe a minimum dose of 47.5 Gy to greater than 90% of the POV and to minimize the POV and/or normal tissue receiving a dose greater than 107%. When treatment goals were not achieved by using a 4-field technique (2 conventional open plus 2 IMRT tangents), a 6-field technique was applied, adding 2 non tangential (anterior-oblique) IMRT beams. Results: Using scripts, manual procedures were minimized (choice of optimal beam angle, setting monitor units for open tangentials, and POV definition). Treatment goals were achieved by using the 4-field technique in 61 of 78 (78%) patients. The 6-field technique was applied in the remaining 17 of 78 (22%) patients, allowing for significantly better achievement of goals, at the expense of an increase of low-dose (∼5 Gy) distribution in the contralateral tissue, heart, and lungs but with no significant increase of higher doses (∼20 Gy) in heart and lungs. The mean monitor unit contribution to IMRT beams was significantly greater (18.7% vs 9.9%) in the group of patients who required 6-field procedure. Conclusions: Because hybrid IMRT can be performed semiautomatically, it can be planned for a large number of patients with little impact on human or departmental resources, promoting it as the standard practice for whole-breast irradiation.

  14. The Development of On-Line Statistics Program for Radiation Oncology

    International Nuclear Information System (INIS)

    Kim, Yoon Jong; Lee, Dong Hoon; Ji, Young Hoon; Lee, Dong Han; Jo, Chul Ku; Kim, Mi Sook; Ru, Sung Rul; Hong, Seung Hong

    2001-01-01

    Purpose : By developing on-line statistics program to record the information of radiation oncology to share the information with internet. It is possible to supply basic reference data for administrative plans to improve radiation oncology. Materials and methods : The information of radiation oncology statistics had been collected by paper forms about 52 hospitals in the past. Now, we can input the data by internet web browsers. The statistics program used windows NT 4.0 operation system, Internet Information Server 4.0 (IIS4.0) as a web server and the Microsoft Access MDB. We used Structured Query Language (SQL), Visual Basic, VBScript and JAVAScript to display the statistics according to years and hospitals. Results : This program shows present conditions about man power, research, therapy machines, technic, brachytherapy, clinic statistics, radiation safety management, institution, quality assurance and radioisotopes in radiation oncology department. The database consists of 38 inputs and 6 outputs windows. Statistical output windows can be increased continuously according to user need. Conclusion : We have developed statistics program to process all of the data in department of radiation oncology for reference information. Users easily could input the data by internet web browsers and share the information

  15. Processing line for industrial radiation-thermal synthesis of doped lithium ferrite powders

    Science.gov (United States)

    Surzhikov, A. P.; Galtseva, O. V.; Vasendina, E. A.; Vlasov, V. A.; Nikolaev, E. V.

    2016-02-01

    The paper considers the issues of industrial production of doped lithium ferrite powders by radiation-thermal method. A technological scheme of the processing line is suggested. The radiation-thermal technological scheme enables production of powders with technical characteristics close to the required ones under relatively low temperature annealing conditions without intermediate mixing. The optimal conditions of the radiation-thermal synthesis are achieved isothermally under irradiation by the electron beam with energy of 2.5 MeV in the temperature range of 700-750 0C within- 120 min.

  16. Transcriptome-wide studies of prostate cancer cell lines in the context of medical radiation

    International Nuclear Information System (INIS)

    Hammer, Paul

    2012-01-01

    The use of radiotherapy in addition to chemotherapy and surgical removal is the most powerful instrument in the fight against malignant tumors in cancer medicine. After cardiovascular diseases, cancer is the second leading cause of death in the western world, in which prostate cancer is the most frequent male cancer. Despite continuous technological improvements in radiological instruments and prognosis, it may occur a recurrence up to many years after radiotherapy due to a high resistance capability of individual malignant cells of the locally occurring tumor. Although modern radiation biology has studied many aspects of the resistance mechanisms, questions are largely unanswered especially in regards to prognostic terms and time response of tumor cells to ionizing radiation. As cellular models four prostate cancer cell lines with different radiation sensitivities (PC3, DuCaP, DU-145, RWPE-1) were cultured and tested for their ability to survive after exposure to ionizing radiation by a trypane blue and MTT viability assay. The proliferative capacity of the four cell lines was determined using a colony formation assay. The PC3 cell line (radiation-resistant) and the DuCaP cell line (radiation-sensitive) showed the maximal differences in terms of radiation sensitivity. Based on these results the two cell lines were selected to allow identification of potential prognostic marker for predicting the effectiveness of radiation therapy via their transcriptome-wide gene expression. Furthermore, a time series experiment with the radiation-resistant PC3 cell line was performed. At 8 different time points, during the period from 00:00 - 42:53 (hh:mm) after exposure with 1 Gy, the mRNA was quantified by next generation sequencing to investigate the dynamic behavior of time-delayed gene expression and to discover resistance mechanisms. Of 10,966 expressed genes 730 were significant differentially expressed, determined by setting a fold change threshold in conjunction with a P

  17. Kelussia odoratissima potentiates cytotoxic effects of radiation in HeLa cancer cell line

    Directory of Open Access Journals (Sweden)

    Azar Hosseini

    2017-02-01

    Full Text Available Objective: Cervical cancer is the second most common cause of death from cancer in women throughout the world. The aim of this study was to evaluate the cytotoxic activity of Kelussia odoratissima (K. odoratissima extract associated with radiotherapy in cervical cancer cells (HeLa cell line.Materials and Methods: Different concentration of the extract (25-500µg/ml was tested in HeLa cell lines. Cell cytotoxicity of the extract and the effects of the extract on radiation (2Gy/min-induced damages were assessed by MTT assay. Apoptosis was assessed using flow cytometric analysis.Result: K. odoratissima decreased cell viability in HeLa cell line in a concentration and time-dependent manner. When compared to the control,K. odoratissima induced a sub-G1 peak in the flow cytometry histogram of treated cells, indicating that apoptotic cell death is involved in K. odoratissima-induced toxicity. It was also shown that K. odoratissima sensitizes cells to radiation-induced toxicity.Conclusion: Our result showed the extract increased the radiation effect. This observation may be related to the presence of active compounds such as phthalides and ferulic acid.

  18. The monitoring results of electromagnetic radiation of 110-kV high-voltage lines in one urban location in Chongqing P.R. China.

    Science.gov (United States)

    Qin, Qi-Zhong; Chen, Yu; Fu, Ting-Ting; Ding, Li; Han, Ling-Li; Li, Jian-Chao

    2012-03-01

    To understand electromagnetic radiation field strength and its influencing factors of certain 110-kV high-voltage lines in one urban area of Chongqing by measuring 110-kV high-voltage line's electromagnetic radiation level. According to the methodology as determined by the National Hygienic Standards, we selected certain adjacent residential buildings, high-voltage lines along a specific street and selected different distances around its vertical projection point as monitoring points. The levels of electromagnetic radiations were measured respectively. In this investigation within the frequency of 5-1,000 Hz both the electric field strength and magnetic field strength of each monitoring sites were lower than the public exposure standards as determined by the International Commission on Non-Ionizing Radiation Protection. However, the electrical field strength on the roof adjacent to the high-voltage lines was significantly higher than that as measured on the other floors in the same buildings (p electromagnetic radiation measurements of different monitoring points, under the same high-voltage lines, showed the location which is nearer the high-voltage line maintain a consistently higher level of radiation than the more distant locations (p Electromagnetic radiation generated by high-voltage lines decreases proportionally to the distance from the lines. The buildings can to some extent shield (or absorb) the electric fields generated by high-voltage lines nearby. The electromagnetic radiation intensity near high-voltage lines may be mitigated or intensified by the manner in which the high-voltage lines are set up, and it merits attention for the potential impact on human health.

  19. FPGA implementation of a hybrid on-line process monitoring in PC based real-time systems

    Directory of Open Access Journals (Sweden)

    Jovanović Bojan

    2011-01-01

    Full Text Available This paper presents one way of FPGA implementation of hybrid (hardware-software based on-line process monitoring in Real-Time systems (RTS. The reasons for RTS monitoring are presented at the beginning. The summary of different RTS monitoring approaches along with its advantages and drawbacks are also exposed. Finally, monitoring module is described in details. Also, FPGA implementation results and some useful monitoring system applications are mentioned.

  20. Yield performance and stability of CMS-based triticale hybrids.

    Science.gov (United States)

    Mühleisen, Jonathan; Piepho, Hans-Peter; Maurer, Hans Peter; Reif, Jochen Christoph

    2015-02-01

    CMS-based triticale hybrids showed only marginal midparent heterosis for grain yield and lower dynamic yield stability compared to inbred lines. Hybrids of triticale (×Triticosecale Wittmack) are expected to possess outstanding yield performance and increased dynamic yield stability. The objectives of the present study were to (1) examine the optimum choice of the biometrical model to compare yield stability of hybrids versus lines, (2) investigate whether hybrids exhibit a more pronounced grain yield performance and yield stability, and (3) study optimal strategies to predict yield stability of hybrids. Thirteen female and seven male parental lines and their 91 factorial hybrids as well as 30 commercial lines were evaluated for grain yield in up to 20 environments. Hybrids were produced using a cytoplasmic male sterility (CMS)-inducing cytoplasm that originated from Triticumtimopheevii Zhuk. We found that the choice of the biometrical model can cause contrasting results and concluded that a group-by-environment interaction term should be added to the model when estimating stability variance of hybrids and lines. midparent heterosis for grain yield was on average 3 % with a range from -15.0 to 11.5 %. No hybrid outperformed the best inbred line. Hybrids had, on average, lower dynamic yield stability compared to the inbred lines. Grain yield performance of hybrids could be predicted based on midparent values and general combining ability (GCA)-predicted values. In contrast, stability variance of hybrids could be predicted only based on GCA-predicted values. We speculated that negative effects of the used CMS cytoplasm might be the reason for the low performance and yield stability of the hybrids. For this purpose a detailed study on the reasons for the drawback of the currently existing CMS system in triticale is urgently required comprising also the search of potentially alternative hybridization systems.

  1. Development of a Indica rice restorer line Fuhui838 and its derivative lines with strong restoring ability and their utilization

    International Nuclear Information System (INIS)

    Deng Dasheng; Chen Hao; Deng Wenmin; Feng Lei; Yang Xuefeng; Ma Anlu; Liu Minghai

    2009-01-01

    Fuhui 838 and its derivative restorer lines (Fuhui 718, Fuhui 305, Zhongfu 218, Mianhui 3728, Nuoyou 1...) have the ability of strong restoring, high combining, and good resisting, which were bred and released by combined techniques of radiation-induced mutation, polymerization hybridization, and temperature tolerance screening. At present, about 43 combinations were registered and released to commercial production, the series of combinations present high seed setting rate, good tolerance to chilling and heat stress, wide adaptability, high and stable yield, the accumulated area added up to 40 million hm 2 . II you 838, which is famous one of these combinations, proved to be the best in long term and wide planting after Sanyou 63, and it is also one of the main hybrid rice for exporting in China. (authors)

  2. New wiggler beam line for SSRL

    International Nuclear Information System (INIS)

    Hoyer, E.

    1982-08-01

    A new high-intensity-beam line with a wiggler magnet source is described. This project, in final stages of design, is a joint effort between Lawrence Berkeley Laboratory (LBL), the Exxon Research and Engineering Company (EXXON), and the Stanford Synchrotron Radiation Laboratory (SSRL). Installation at SSRL will begin in the summer of 1982. The goal of this project is to provide extremely high-brightness synchrotron radiation beams over a broad spectral range from 50 eV to 40 keV. The radiation source is a 27 period (i.e., 55 pole) permanent magnet wiggler of a new design. The wiggler utilizes rare-earth cobalt (REC) material in the steel hybrid configuration to achieve high magnetic fields with short periods. An analysis has been made of the polarization, angular distribution and power density of the radiation produced by the wiggler. Details of the wiggler design are presented. The magnet is outside a thin walled (1mm) variable gap stainless steel vacuum chamber. The chamber gap will be opened to 1.8 cm for beam injection into SPEAR and then closed to 1.0 cm (or less) for operation. Five remotely controlled drives are provided; to change the wiggler gap, to change the vacuum chamber aperture and to position the wiggler. Details of the beam line optics and end stations are presented. Thermal loading on beam line components is severe. The peak power density at 7.5 m is 5 kW/cm 2 for the nominal wiggler field and present SPEAR beam currents and will approach 20 kW/cm 2 with the maximum wiggler field and projected SPEAR beam currents

  3. Heavy metal incorporated helium ion active hybrid non-chemically amplified resists: Nano-patterning with low line edge roughness

    Directory of Open Access Journals (Sweden)

    Pulikanti Guruprasad Reddy

    2017-08-01

    Full Text Available Helium (He ion lithography is being considered as one of the most promising and emerging technology for the manufacturing of next generation integrated circuits (ICs at nanolevel. However, He-ion active resists are rarely reported. In this context, we are introducing a new non-chemically amplified hybrid resist (n-CAR, MAPDSA-MAPDST, for high resolution He-ion beam lithography (HBL applications. In the resist architecture, 2.15 % antimony is incorporated as heavy metal in the form of antimonate. This newly developed resists has successfully used for patterning 20 nm negative tone features at a dose of 60 μC/cm2. The resist offered very low line edge roughness (1.27±0.31 nm for 20 nm line features. To our knowledge, this is the first He-ion active hybrid resist for nanopatterning. The contrast (γ and sensitivity (E0 of this resist were calculated from the contrast curve as 0.73 and 7.2 μC/cm2, respectively.

  4. Heavy metal incorporated helium ion active hybrid non-chemically amplified resists: Nano-patterning with low line edge roughness

    Science.gov (United States)

    Reddy, Pulikanti Guruprasad; Thakur, Neha; Lee, Chien-Lin; Chien, Sheng-Wei; Pradeep, Chullikkattil P.; Ghosh, Subrata; Tsai, Kuen-Yu; Gonsalves, Kenneth E.

    2017-08-01

    Helium (He) ion lithography is being considered as one of the most promising and emerging technology for the manufacturing of next generation integrated circuits (ICs) at nanolevel. However, He-ion active resists are rarely reported. In this context, we are introducing a new non-chemically amplified hybrid resist (n-CAR), MAPDSA-MAPDST, for high resolution He-ion beam lithography (HBL) applications. In the resist architecture, 2.15 % antimony is incorporated as heavy metal in the form of antimonate. This newly developed resists has successfully used for patterning 20 nm negative tone features at a dose of 60 μC/cm2. The resist offered very low line edge roughness (1.27±0.31 nm) for 20 nm line features. To our knowledge, this is the first He-ion active hybrid resist for nanopatterning. The contrast (γ) and sensitivity (E0) of this resist were calculated from the contrast curve as 0.73 and 7.2 μC/cm2, respectively.

  5. TU-G-BRA-08: BEST IN PHYSICS (JOINT IMAGING-THERAPY): Hybrid PET-MRI Imaging of Acute Radiation Induced Cardiac Toxicity

    International Nuclear Information System (INIS)

    El-Sherif, O; Xhaferllari, I; Gaede, S; Sykes, J; Butler, J; Wisenberg, G; Prato, F

    2015-01-01

    Purpose: To identify the presence of low-dose radiation induced cardiac toxicity in a canine model using hybrid positron emission tomography (PET) and magnetic resonance imaging (MRI). Methods: Research ethics board approval was obtained for a longitudinal imaging study of 5 canines after cardiac irradiation. Animals were imaged at baseline, 1 week post cardiac irradiation, and 1 month post cardiac irradiation using a hybrid PET- MRI system (Biograph mMR, Siemens Healthcare). The imaging protocol was designed to assess acute changes in myocardial perfusion and inflammation. Myocardial perfusion imaging was performed using N13-ammonia tracer followed by a dynamic PET acquisition scan. A compartmental tracer kinetic model was used for absolute perfusion quantification. Myocardial inflammation imaging was performed using F18-fluorodeoxyglucose (FDG) tracer. The standard uptake value (SUV) over a region encompassing the whole heart was used to compare FDG scans. All animals received a simulation CT scan (GE Medical Systems) for radiation treatment planning. Radiation treatment plans were created using the Pinncale3 treatment planning system (Philips Radiation Oncology Systems) and designed to resemble the typical cardiac exposure during left-sided breast cancer radiotherapy. Cardiac irradiations were performed in a single fraction using a TrueBeam linear accelerator (Varian Medical Systems). Results: The delivered dose (mean ± standard deviation) to heart was 1.8±0.2 Gy. Reductions in myocardial stress perfusion relative to baseline were observed in 2 of the 5 animals 1 month post radiation. A global inflammatory response 1 month post radiation was observed in 4 of the 5 animals. The calculated SUV at 1 month post radiation was significantly higher (p=0.05) than the baseline SUV. Conclusion: Low doses of cardiac irradiation (< 2 Gy) may lead to myocardial perfusion defects and a global inflammatory response that can be detectable as early as 1 month post irradiation

  6. TU-G-BRA-08: BEST IN PHYSICS (JOINT IMAGING-THERAPY): Hybrid PET-MRI Imaging of Acute Radiation Induced Cardiac Toxicity

    Energy Technology Data Exchange (ETDEWEB)

    El-Sherif, O; Xhaferllari, I; Gaede, S [Western Univeristy, London, ON (United Kingdom); London Regional Cancer Program, London, ON (United Kingdom); Sykes, J; Butler, J [Lawson Health Research Institute, London, ON (United Kingdom); Wisenberg, G; Prato, F [Western Univeristy, London, ON (United Kingdom); Lawson Health Research Institute, London, ON (United Kingdom)

    2015-06-15

    Purpose: To identify the presence of low-dose radiation induced cardiac toxicity in a canine model using hybrid positron emission tomography (PET) and magnetic resonance imaging (MRI). Methods: Research ethics board approval was obtained for a longitudinal imaging study of 5 canines after cardiac irradiation. Animals were imaged at baseline, 1 week post cardiac irradiation, and 1 month post cardiac irradiation using a hybrid PET- MRI system (Biograph mMR, Siemens Healthcare). The imaging protocol was designed to assess acute changes in myocardial perfusion and inflammation. Myocardial perfusion imaging was performed using N13-ammonia tracer followed by a dynamic PET acquisition scan. A compartmental tracer kinetic model was used for absolute perfusion quantification. Myocardial inflammation imaging was performed using F18-fluorodeoxyglucose (FDG) tracer. The standard uptake value (SUV) over a region encompassing the whole heart was used to compare FDG scans. All animals received a simulation CT scan (GE Medical Systems) for radiation treatment planning. Radiation treatment plans were created using the Pinncale3 treatment planning system (Philips Radiation Oncology Systems) and designed to resemble the typical cardiac exposure during left-sided breast cancer radiotherapy. Cardiac irradiations were performed in a single fraction using a TrueBeam linear accelerator (Varian Medical Systems). Results: The delivered dose (mean ± standard deviation) to heart was 1.8±0.2 Gy. Reductions in myocardial stress perfusion relative to baseline were observed in 2 of the 5 animals 1 month post radiation. A global inflammatory response 1 month post radiation was observed in 4 of the 5 animals. The calculated SUV at 1 month post radiation was significantly higher (p=0.05) than the baseline SUV. Conclusion: Low doses of cardiac irradiation (< 2 Gy) may lead to myocardial perfusion defects and a global inflammatory response that can be detectable as early as 1 month post irradiation

  7. Studies of radioinduced mutations in sorghum grain: 1. Comparison of phenotypic variability obtained through hybridation and mutagenesis of F2 and M2 populations; 2. Agronomical and physiotechnical characterization of mutants lines in the original collection and in the advanced lines of the Chapingo Postgraduate college

    International Nuclear Information System (INIS)

    Parra Negrete, L.A.

    1986-01-01

    Genetic inprovement of cultivated plants consists essentially of three phases (1) generation of genetic variability (2) selection of genotypes and (3) evaluation of selected genotypes. Hybridization and spontaneous or induced mutations are, responsible for the generation of and increase in genetic vegetative variability. Accordingly, such methods are used alternatively in local programs for plant improvement either for introducing improved genotypes or as sources of germplasma. This thesis is based on two experiments of mutations induced by cobalt 60 ionizing radiation while using distinct materials and methods presented in two parts, the first section analyze the mutation variability and the second the evaluation of mutant lines. (author)

  8. Rapid radiations of both kiwifruit hybrid lineages and their parents shed light on a two-layer mode of species diversification.

    Science.gov (United States)

    Liu, Yifei; Li, Dawei; Zhang, Qiong; Song, Chi; Zhong, Caihong; Zhang, Xudong; Wang, Ying; Yao, Xiaohong; Wang, Zupeng; Zeng, Shaohua; Wang, Ying; Guo, Yangtao; Wang, Shuaibin; Li, Xinwei; Li, Li; Liu, Chunyan; McCann, Honour C; He, Weiming; Niu, Yan; Chen, Min; Du, Liuwen; Gong, Junjie; Datson, Paul M; Hilario, Elena; Huang, Hongwen

    2017-07-01

    Reticulate speciation caused by interspecific hybridization is now recognized as an important mechanism in the creation of biological diversity. However, depicting the patterns of phylogenetic networks for lineages that have undergone interspecific gene flow is challenging. Here we sequenced 25 taxa representing natural diversity in the genus Actinidia with an average mapping depth of 26× on the reference genome to reconstruct their reticulate history. We found evidence, including significant gene tree discordance, cytonuclear conflicts, and changes in genome-wide heterozygosity across taxa, collectively supporting extensive reticulation in the genus. Furthermore, at least two separate parental species pairs were involved in the repeated origin of the hybrid lineages, in some of which a further phase of syngameon was triggered. On the basis of the elucidated hybridization relationships, we obtained a highly resolved backbone phylogeny consisting of taxa exhibiting no evidence of hybrid origin. The backbone taxa have distinct demographic histories and are the product of recent rounds of rapid radiations via sorting of ancestral variation under variable climatic and ecological conditions. Our results suggest a mode for consecutive plant diversification through two layers of radiations, consisting of the rapid evolution of backbone lineages and the formation of hybrid swarms derived from these lineages. © 2017 The Authors. New Phytologist © 2017 New Phytologist Trust.

  9. Radiation Changes the Metabolic Profiling of Melanoma Cell Line B16.

    Directory of Open Access Journals (Sweden)

    Lige Wu

    Full Text Available Radiation therapy can be an effective way to kill cancer cells using ionizing radiation, but some tumors are resistant to radiation therapy and the underlying mechanism still remains elusive. It is therefore necessary to establish an appropriate working model to study and monitor radiation-mediated cancer therapy. In response to cellular stress, the metabolome is the integrated profiling of changes in all metabolites in cells, which can be used to investigate radiation tolerance mechanisms and identify targets for cancer radiation sensibilization. In this study, using 1H nuclear magnetic resonance for untargeted metabolic profiling in radiation-tolerant mouse melanoma cell line B16, we comprehensively investigated changes in metabolites and metabolic network in B16 cells in response to radiation. Principal component analysis and partial least squares discriminant analysis indicated the difference in cellular metabolites between the untreated cells and X-ray radiated cells. In radiated cells, the content of alanine, glutamate, glycine and choline was increased, while the content of leucine, lactate, creatine and creatine phosphate was decreased. Enrichment analysis of metabolic pathway showed that the changes in metabolites were related to multiple metabolic pathways including the metabolism of glycine, arginine, taurine, glycolysis, and gluconeogenesis. Taken together, with cellular metabolome study followed by bioinformatic analysis to profile specific metabolic pathways in response to radiation, we deepened our understanding of radiation-resistant mechanisms and radiation sensibilization in cancer, which may further provide a theoretical and practical basis for personalized cancer therapy.

  10. Breeding of early restorer Fuhui 306 and predominant performance of F1 hybrid combinations

    International Nuclear Information System (INIS)

    Wu Wanyi; Liu Yongqiang; Wu Maoli; Xue Xingqiong

    2004-01-01

    Fuhui 306, an early rice restorer with strong restoring ability, short growth period (86 days) and no restriction of separating areas in seed production, was bred by radiation treatment. F 1 hybrid combinations with different mature period were developed when cross with different sterile lines, that combination would be widely applied to meet the requirement of different ecological environment and harvest period. (authors)

  11. Design and application of radiation apparatus for sup 6 sup 0 Co cargo train on-line inspection system

    CERN Document Server

    Wu Zhi Fang; Zhang Yuai

    2002-01-01

    Based on the special requirement for radiation apparatus of sup 6 sup 0 Co cargo train on-line inspection system, a radiation apparatus including two-level shutter, working container, storing container and electromagnetism are designed. The makeup, working mode and functional realization of the radiation apparatus are introduced. The system is used in Manzhouli customs cargo train on-line inspection system. The practice shows that the radiation apparatus is reliable to work steadily and the operating speed of the main shutter can reach 0.1 s open and 0.15 s close

  12. In vitro study of combined cilengitide and radiation treatment in breast cancer cell lines

    International Nuclear Information System (INIS)

    Lautenschlaeger, Tim; Perry, James; Peereboom, David; Li, Bin; Ibrahim, Ahmed; Huebner, Alexander; Meng, Wei; White, Julia; Chakravarti, Arnab

    2013-01-01

    Brain metastasis from breast cancer poses a major clinical challenge. Integrins play a role in regulating adhesion, growth, motility, and survival, and have been shown to be critical for metastatic growth in the brain in preclinical models. Cilengitide, an αvβ3/αvβ5 integrin inhibitor, has previously been studied as an anti-cancer drug in various tumor types. Previous studies have shown additive effects of cilengitide and radiation in lung cancer and glioblastoma cell lines. The ability of cilengitide to enhance the effects of radiation was examined preclinically in the setting of breast cancer to assess its possible efficacy in the setting of brain metastasis from breast cancer. Our panel of breast cells was composed of four cell lines: T-47D (ER/PR+, Her2-, luminal A), MCF-7 (ER/PR+, Her2-, luminal A), MDA-MB-231 (TNBC, basal B), MDA-MB-468 (TNBC, basal A). The presence of cilengitide targets, β3 and β5 integrin, was first determined. Cell detachment was determined by cell counting, cell proliferation was determined by MTS proliferation assay, and apoptosis was measured by Annexin V staining and flow cytometry. The efficacy of cilengitide treatment alone was analyzed, followed by assessment of combined cilengitide and radiation treatment. Integrin β3 knockdown was performed, followed by cilengitide and radiation treatment to test for incomplete target inhibition by cilengitide, in high β3 expressing cells. We observed that all cell lines examined expressed both β3 and β5 integrin and that cilengitide was able to induce cell detachment and reduced proliferation in our panel. Annexin V assays revealed that a portion of these effects was due to cilengitide-induced apoptosis. Combined treatment with cilengitide and radiation served to further reduce proliferation compared to either treatment alone. Following β3 integrin knockdown, radiosensitization in combination with cilengitide was observed in a previously non-responsive cell line (MDA-MB-231

  13. Advances in hybrid optics physical sensors for extreme environments

    Science.gov (United States)

    Riza, Nabeel A.

    2010-04-01

    Highlighted are novel innovations in hybrid optical design physical sensors for extreme environments. Various hybrid design compositions are proposed that are suited for a particular sensor application. Examples includes combining freespace (wireless) and fiber-optics (wired) for gas turbine sensing and combining single crystal and sintered Silicon Carbide (SiC) materials for robust extreme environment Coefficent of Thermal Expansion (CTE) matched frontend probe design. Sensor signal processing also includes the hybrid theme where for example Black-Body radiation thermometry (pyrometry) is combined with laser interferometry to provide extreme temperature measurements. The hybrid theme also operates on the optical device level where a digital optical device such as a Digital Micromirror Device (DMD) is combined with an analog optical device such as an Electronically Controlled Variable Focal Length Lens (ECVFL) to deliver a smart and compressive Three Dimensional (3-D) imaging sensor for remote scene and object shape capture including both ambient light (passive) mode and active laser targeting and receive processing. Within a device level, the hybrid theme also operates via combined analog and digital control such as within a wavelength-coded variable optical delay line. These powerful hybrid design optical sensors have numerous applications in engineering and science applications from the military to the commercial/industrial sectors.

  14. Heterogeneity in 2-deoxy-D-glucose-induced modifications in energetics and radiation responses of human tumor cell lines

    International Nuclear Information System (INIS)

    Dwarkanath, Bilikere S.; Zolzer, Frido; Chandana, Sudhir; Bauch, Thomas; Adhikari, Jawahar S.; Muller, Wolfgang U.; Streffer, Christian; Jain, Viney

    2001-01-01

    Purpose: The glucose analog and glycolytic inhibitor, 2-deoxy-D-glucose (2-DG), has been shown to differentially enhance the radiation damage in tumor cells by inhibiting the postirradiation repair processes. The present study was undertaken to examine the relationship between 2-DG-induced modification of energy metabolism and cellular radioresponses and to identify the most relevant parameter(s) for predicting the tumor response to the combined treatment of radiation + 2-DG. Methods and Materials: Six human tumor cell lines (glioma: BMG-1 and U-87, squamous cell carcinoma: 4451 and 4197, and melanoma: MeWo and Be-11) were investigated. Cells were exposed to 2 Gy of Co-60 γ-rays or 250 kVP X-rays and maintained under liquid-holding conditions 2-4 h to facilitate repair. 2-DG (5 mM, equimolar with glucose) that was added at the time of irradiation was present during the liquid holding. Glucose utilization, lactate production (enzymatic assays), and adenine nucleotides (high performance liquid chromatography and capillary isotachophoresis) were investigated as parameters of energy metabolism. Induction and repair of DNA damage (comet assay), cytogenetic damage (micronuclei formation), and cell death (macrocolony assay) were analyzed as parameters of radiation response. Results: The glucose consumption and lactate production of glioma cell lines (BMG-1 and U-87) were nearly 2-fold higher than the squamous carcinoma cell lines (4197 and 4451). The ATP content varied from 3.0 to 6.5 femto moles/cell among these lines, whereas the energy charge (0.86-0.90) did not show much variation. Presence of 2-DG inhibited the rate of glucose usage and glycolysis by 30-40% in glioma cell lines and by 15-20% in squamous carcinoma lines, while ATP levels reduced by nearly 40% in all the four cell lines. ATP:ADP ratios decreased to a greater extent (∼40%) in glioma cells than in squamous carcinoma 4451 and MeWo cells; in contrast, presence of 2-DG reduced ADP:AMP ratios by 3-fold in

  15. Chromosome identification by new molecular markers and genomic in situ hybridization in the Triticum-Secale-Thinopyrum trigeneric hybrids.

    Science.gov (United States)

    Dai, Yi; Duan, Yamei; Chi, Dawn; Liu, Huiping; Huang, Shuai; Cao, Wenguang; Gao, Yong; Fedak, George; Chen, Jianmin

    2017-08-01

    It is very important to use chromosome-specific markers for identifying alien chromosomes in advanced generations of distant hybridization. The chromosome-specific markers of rye and Thinopyrum elongatum, as well as genomic in situ hybridization, were used to identify the alien chromosomes in eight lines that were derived from the crossing between Triticum trititrigia (AABBEE) and triticale (AABBRR). The results showed that four lines contained all rye chromosomes but no Th. elongatum chromosomes. The line RE36-1 contained all of the rye chromosomes except for chromosome 2R. The lines RE33-2 and RE62-1 contained all rye chromosomes and 1E and 5E translocated chromosome, respectively. The line RE24-4 contained 12 rye chromosomes plus a 7E chromosome or 12 rye chromosomes plus one R-E translocated chromosome. Chromosome identification in the above lines was consistent using chromosome-specific markers and genomic in situ hybridization. These chromosome-specific markers provide useful tools for detecting alien chromosomes in trigeneric hybrids, and these lines could be utilized as valuable germplasm in wheat improvement.

  16. THE ROLE OF RADIATION PRESSURE IN THE NARROW LINE REGIONS OF SEYFERT HOST GALAXIES

    Energy Technology Data Exchange (ETDEWEB)

    Davies, Rebecca L.; Dopita, Michael A.; Kewley, Lisa; Groves, Brent; Sutherland, Ralph; Hampton, Elise J.; Banfield, Julie [Research School of Astronomy and Astrophysics, Australian National University, Canberra, ACT 2611 (Australia); Shastri, Prajval; Kharb, Preeti; Bhatt, Harish [Indian Institute of Astrophysics, Sarjapur Road, Bengaluru 560034 (India); Scharwächter, Julia [LERMA, Observatoire de Paris, PSL, CNRS, Sorbonne Universités, UPMC, F-75014 Paris (France); Jin, Chichuan [Max-Planck-Institut für Extraterrestrische Physik, Giessenbachstrasse, D-85748 Garching (Germany); Zaw, Ingyin [New York University (Abu Dhabi), 70 Washington Square S, New York, NY 10012 (United States); James, Bethan [Institute of Astronomy, Cambridge University, Madingley Road, Cambridge CB3 0HA (United Kingdom); Juneau, Stéphanie [CEA-Saclay, DSM/IRFU/SAp, F-91191 Gif-sur-Yvette (France); Srivastava, Shweta, E-mail: Rebecca.Davies@anu.edu.au [Astronomy and Astrophysics Division, Physical Research Laboratory, Ahmedabad 380009 (India)

    2016-06-10

    We investigate the relative significance of radiation pressure and gas pressure in the extended narrow line regions (ENLRs) of four Seyfert galaxies from the integral field Siding Spring Southern Seyfert Spectroscopic Snapshot Survey (S7). We demonstrate that there exist two distinct types of starburst-active galactic nucleus (AGN) mixing curves on standard emission line diagnostic diagrams, which reflect the balance between gas pressure and radiation pressure in the ENLR. In two of the galaxies the ENLR is radiation pressure dominated throughout and the ionization parameter remains constant (log U ∼ 0). In the other two galaxies radiation pressure is initially important, but gas pressure becomes dominant as the ionization parameter in the ENLR decreases from log U ∼ 0 to −3.2 ≲ log U ≲ −3.4. Where radiation pressure is dominant, the AGN regulates the density of the interstellar medium on kiloparsec scales and may therefore have a direct impact on star formation activity and/or the incidence of outflows in the host galaxy to scales far beyond the zone of influence of the black hole. We find that both radiation pressure dominated and gas pressure dominated ENLRs are dynamically active with evidence for outflows, indicating that radiation pressure may be an important source of AGN feedback even when it is not dominant over the entire ENLR.

  17. Radiation by the numbers: developing an on-line Canadian radiation dose calculator as a public engagement and education tool

    Energy Technology Data Exchange (ETDEWEB)

    Dalzell, M.T.J. [Sylvia Fedoruk Canadian Centre for Nuclear Innovation, Saskatoon, Saskatchewan (Canada)

    2016-06-15

    Concerns arising from misunderstandings about radiation are often cited as a main reason for public antipathy towards nuclear development and impede decision-making by governments and individuals. A lack of information about everyday sources of radiation exposure that is accessible, relatable and factual contributes to the problem. As part of its efforts to be a fact-based source of information on nuclear issues, the Sylvia Fedoruk Canadian Centre for Nuclear Innovation has developed an on-line Canadian Radiation Dose Calculator as a tool to provide context about common sources of radiation. This paper discusses the development of the calculator and describes how the Fedoruk Centre is using it and other tools to support public engagement on nuclear topics. (author)

  18. Source of broadband Jovian Kilometric radiation

    Energy Technology Data Exchange (ETDEWEB)

    Jones, D.; Leblanc, Y.

    1987-02-01

    Broadband Jovian Kilometric radiation was observed by Voyagers 1 and 2 to be beamed away from the zenomagnetic equatorial plane. Two theories were proposed for the equatorial shadow zone. One suggested that Io plasma torus forms an obstacle to radiation produced on auroral field lines. The other theory proposed that the source is located on the outer flanks of the torus, the beaming being inherent to the emission mechanism. Results are presented which indicate that the latter is consistent with the observations and it would appear that the emission is produced by linear mode conversion of electrostatic upper hybrid to electromagnetic waves in plasma density gradients.

  19. Source of broadband Jovian Kilometric radiation

    International Nuclear Information System (INIS)

    Jones, D.; Leblanc, Y.

    1987-01-01

    Broadband Jovian Kilometric radiation was observed by Voyagers 1 and 2 to be beamed away from the zenomagnetic equatorial plane. Two theories were proposed for the equatorial shadow zone. One suggested that Io plasma torus forms an obstacle to radiation produced on auroral field lines. The other theory proposed that the source is located on the outer flanks of the torus, the beaming being inherent to the emission mechanism. Results are presented which indicate that the latter is consistent with the observations and it would appear that the emission is produced by linear mode conversion of electrostatic upper hybrid to electromagnetic waves in plasma density gradients

  20. On beam shaping of the field radiated by a line source coupled to finite or infinite photonic crystals.

    Science.gov (United States)

    Ceccuzzi, Silvio; Jandieri, Vakhtang; Baccarelli, Paolo; Ponti, Cristina; Schettini, Giuseppe

    2016-04-01

    Comparison of the beam-shaping effect of a field radiated by a line source, when an ideal infinite structure constituted by two photonic crystals and an actual finite one are considered, has been carried out by means of two different methods. The lattice sums technique combined with the generalized reflection matrix method is used to rigorously investigate the radiation from the infinite photonic crystals, whereas radiation from crystals composed of a finite number of rods along the layers is analyzed using the cylindrical-wave approach. A directive radiation is observed with the line source embedded in the structure. With an increased separation distance between the crystals, a significant edge diffraction appears that provides the main radiation mechanism in the finite layout. Suitable absorbers are implemented to reduce the above-mentioned diffraction and the reflections at the boundaries, thus obtaining good agreement between radiation patterns of a localized line source coupled to finite and infinite photonic crystals, when the number of periods of the finite structure is properly chosen.

  1. X-ray diffraction using synchrotron radiation on the G.I.L.D.A. beam line at the E.S.R.F

    Energy Technology Data Exchange (ETDEWEB)

    Balerna, A [INFN, Laboratori Nazionali di Frascati, Rome (Italy); Meneghini, C [INFN, Laboratori Nazionali di Frascati, Rome (Italy); [INFM, Genoa (Italy); Bordoni, S [Rome Univ. ` Tor Vergata` (Italy). Dip. di Fisica; Mobilio, S [Rome Univ. III (Italy). Dip. di Fisica ` E. Amaldi`

    1996-09-01

    The aim of this lecture is to make a short introduction on Synchrotron radiation, its history and main properties. The main components of a synchrotron radiation beam line will be described. The Italian beam line, General purpose Italian beam line Line for Diffraction and Absorption (G.I.L.D.A.) at the European Synchrotron Radiation Facility (E.S.R.F.) in Grenoble will be used as an example. The G.I.L.D.A. diffractometer will be described in detail reporting also some experimental results.

  2. Design of a synchrotron radiation detector for the test beam lines at the Superconducting Super Collider Laboratory

    International Nuclear Information System (INIS)

    Hutton, R.D.

    1994-01-01

    As part of the particle- and momentum-tagging instrumentation required for the test beam lines of the Superconducting Super Collider (SSC), the synchrotron radiation detector (SRD) was designed to provide electron tagging at momentum above 75 GeV. In a parallel effort to the three test beam lines at the SSC, schedule demands required testing and calibration operations to be initiated at Fermilab. Synchrotron radiation detectors also were to be installed in the NM and MW beam lines at Femilab before the test beam lines at the SSC would become operational. The SRD is the last instrument in a series of three used in the SSC test beam fines. It follows a 20-m drift section of beam tube downstream of the last silicon strip detector. A bending dipole just in of the last silicon strip detector produces the synchrotron radiation that is detected in a 50-mm-square cross section NaI crystal. A secondary scintillator made of Bicron BC-400 plastic is used to discriminate whether it is synchrotron radiation or a stray particle that causes the triggering of the NaI crystal's photo multiplier tube (PMT)

  3. Molecular Breeding of Rice Restorer Lines and Hybrids for Brown Planthopper (BPH) Resistance Using the Bph14 and Bph15 Genes.

    Science.gov (United States)

    Wang, Hongbo; Ye, Shengtuo; Mou, Tongmin

    2016-12-01

    The development of hybrid rice is a practical approach for increasing rice production. However, the brown planthopper (BPH), Nilaparvata lugens Stål, causes severe yield loss of rice (Oryza sativa L.) and can threaten food security. Therefore, breeding hybrid rice resistant to BPH is the most effective and economical strategy to maintain high and stable production. Fortunately, numerous BPH resistance genes have been identified, and abundant linkage markers are available for molecular marker-assisted selection (MAS) in breeding programs. Hence, we pyramided two BPH resistance genes, Bph14 and Bph15, into a susceptive CMS restorer line Huahui938 and its derived hybrids using MAS to improve the BPH resistance of hybrid rice. Three near-isogenic lines (NILs) with pyramided Bph14 and Bph15 were obtained by molecular marker-assisted backcross (MAB) and phenotypic selection. The genomic components of these NILs were detected using the whole-genome SNP (Single nucleotide polymorphism) array, RICE6K, suggesting that the recurrent parent genome (RPG) recovery of the NILs was 87.88, 87.70 and 86.62 %, respectively. BPH bioassays showed that the improved NILs and their derived hybrids carrying homozygous Bph14 and Bph15 were resistant to BPH. However, the hybrids with heterozygous Bph14 and Bph15 remained susceptible to BPH. The developed NILs showed no significant differences in major agronomic traits and rice qualities compared with the recurrent parent. Moreover, the improved hybrids derived from the NILs exhibited better agronomic performance and rice quality compared with the controls under natural field conditions. This study demonstrates that it is essential to stack Bph14 and Bph15 into both the maternal and paternal parents for developing BPH-resistant hybrid rice varieties. The SNP array with abundant DNA markers is an efficient tool for analyzing the RPG recovery of progenies and can be used to monitor the donor segments in NILs, thus being extremely important

  4. Radiation loss of planar surface plasmon polaritons transmission lines at microwave frequencies.

    Science.gov (United States)

    Xu, Zhixia; Li, Shunli; Yin, Xiaoxing; Zhao, Hongxin; Liu, Leilei

    2017-07-21

    Radiation loss of a typical spoof surface plasmon polaritons (SSPPs) transmission line (TL) is investigated in this paper. A 325 mm-long SSPPs TL is designed and fabricated. Simulated results show that radiation loss contributes more to transmission loss than dielectric loss and conductor loss from 2 GHz to 10 GHz. Radiation loss of the SSPPs TL could be divided into two parts, one is caused by the input mode converter, and the other is caused by the corrugated metallic strip. This paper explains mechanisms of radiation loss from different parts, designs a loaded SSPPs TL with a series of resistors to absorb electromagnetic energy on corrugated metallic strip, and then discriminates radiation loss from the input mode converter, proposes the concept of average radiation length (ARL) to evaluate radiation loss from SSPPs of finite length, and concludes that radiation loss is mainly caused by corrugated structure of finite length at low frequency band and by the input mode converter at high frequency band. To suppress radiation loss, a mixed slow wave TL based on the combination of coplanar waveguides (CPWs) and SSPPs is presented. The designed structure, sample fabrication and experimental verification are discussed.

  5. The UF family of reference hybrid phantoms for computational radiation dosimetry

    International Nuclear Information System (INIS)

    Lee, Choonsik; Lodwick, Daniel; Hurtado, Jorge; Pafundi, Deanna; Williams, Jonathan L; Bolch, Wesley E

    2010-01-01

    Computational human phantoms are computer models used to obtain dose distributions within the human body exposed to internal or external radiation sources. In addition, they are increasingly used to develop detector efficiencies for in vivo whole-body counters. Two classes of computational human phantoms have been widely utilized for dosimetry calculation: stylized and voxel phantoms that describe human anatomy through mathematical surface equations and 3D voxel matrices, respectively. Stylized phantoms are flexible in that changes to organ position and shape are possible given avoidance of region overlap, while voxel phantoms are typically fixed to a given patient anatomy, yet can be proportionally scaled to match individuals of larger or smaller stature, but of equivalent organ anatomy. Voxel phantoms provide much better anatomical realism as compared to stylized phantoms which are intrinsically limited by mathematical surface equations. To address the drawbacks of these phantoms, hybrid phantoms based on non-uniform rational B-spline (NURBS) surfaces have been introduced wherein anthropomorphic flexibility and anatomic realism are both preserved. Researchers at the University of Florida have introduced a series of hybrid phantoms representing the ICRP Publication 89 reference newborn, 15 year, and adult male and female. In this study, six additional phantoms are added to the UF family of hybrid phantoms-those of the reference 1 year, 5 year and 10 year child. Head and torso CT images of patients whose ages were close to the targeted ages were obtained under approved protocols. Major organs and tissues were segmented from these images using an image processing software, 3D-DOCTOR(TM). NURBS and polygon mesh surfaces were then used to model individual organs and tissues after importing the segmented organ models to the 3D NURBS modeling software, Rhinoceros(TM). The phantoms were matched to four reference datasets: (1) standard anthropometric data, (2) reference

  6. Hourly forecasting of global solar radiation based on multiscale decomposition methods: A hybrid approach

    International Nuclear Information System (INIS)

    Monjoly, Stéphanie; André, Maïna; Calif, Rudy; Soubdhan, Ted

    2017-01-01

    This paper introduces a new approach for the forecasting of solar radiation series at 1 h ahead. We investigated on several techniques of multiscale decomposition of clear sky index K_c data such as Empirical Mode Decomposition (EMD), Ensemble Empirical Mode Decomposition (EEMD) and Wavelet Decomposition. From these differents methods, we built 11 decomposition components and 1 residu signal presenting different time scales. We performed classic forecasting models based on linear method (Autoregressive process AR) and a non linear method (Neural Network model). The choice of forecasting method is adaptative on the characteristic of each component. Hence, we proposed a modeling process which is built from a hybrid structure according to the defined flowchart. An analysis of predictive performances for solar forecasting from the different multiscale decompositions and forecast models is presented. From multiscale decomposition, the solar forecast accuracy is significantly improved, particularly using the wavelet decomposition method. Moreover, multistep forecasting with the proposed hybrid method resulted in additional improvement. For example, in terms of RMSE error, the obtained forecasting with the classical NN model is about 25.86%, this error decrease to 16.91% with the EMD-Hybrid Model, 14.06% with the EEMD-Hybid model and to 7.86% with the WD-Hybrid Model. - Highlights: • Hourly forecasting of GHI in tropical climate with many cloud formation processes. • Clear sky Index decomposition using three multiscale decomposition methods. • Combination of multiscale decomposition methods with AR-NN models to predict GHI. • Comparison of the proposed hybrid model with the classical models (AR, NN). • Best results using Wavelet-Hybrid model in comparison with classical models.

  7. Computational hybrid anthropometric paediatric phantom library for internal radiation dosimetry

    Science.gov (United States)

    Xie, Tianwu; Kuster, Niels; Zaidi, Habib

    2017-04-01

    Hybrid computational phantoms combine voxel-based and simplified equation-based modelling approaches to provide unique advantages and more realism for the construction of anthropomorphic models. In this work, a methodology and C++ code are developed to generate hybrid computational phantoms covering statistical distributions of body morphometry in the paediatric population. The paediatric phantoms of the Virtual Population Series (IT’IS Foundation, Switzerland) were modified to match target anthropometric parameters, including body mass, body length, standing height and sitting height/stature ratio, determined from reference databases of the National Centre for Health Statistics and the National Health and Nutrition Examination Survey. The phantoms were selected as representative anchor phantoms for the newborn, 1, 2, 5, 10 and 15 years-old children, and were subsequently remodelled to create 1100 female and male phantoms with 10th, 25th, 50th, 75th and 90th body morphometries. Evaluation was performed qualitatively using 3D visualization and quantitatively by analysing internal organ masses. Overall, the newly generated phantoms appear very reasonable and representative of the main characteristics of the paediatric population at various ages and for different genders, body sizes and sitting stature ratios. The mass of internal organs increases with height and body mass. The comparison of organ masses of the heart, kidney, liver, lung and spleen with published autopsy and ICRP reference data for children demonstrated that they follow the same trend when correlated with age. The constructed hybrid computational phantom library opens up the prospect of comprehensive radiation dosimetry calculations and risk assessment for the paediatric population of different age groups and diverse anthropometric parameters.

  8. On-line statistical processing of radiation detector pulse trains with time-varying count rates

    International Nuclear Information System (INIS)

    Apostolopoulos, G.

    2008-01-01

    Statistical analysis is of primary importance for the correct interpretation of nuclear measurements, due to the inherent random nature of radioactive decay processes. This paper discusses the application of statistical signal processing techniques to the random pulse trains generated by radiation detectors. The aims of the presented algorithms are: (i) continuous, on-line estimation of the underlying time-varying count rate θ(t) and its first-order derivative dθ/dt; (ii) detection of abrupt changes in both of these quantities and estimation of their new value after the change point. Maximum-likelihood techniques, based on the Poisson probability distribution, are employed for the on-line estimation of θ and dθ/dt. Detection of abrupt changes is achieved on the basis of the generalized likelihood ratio statistical test. The properties of the proposed algorithms are evaluated by extensive simulations and possible applications for on-line radiation monitoring are discussed

  9. Fast neutron radiation induced Glu-B1 deficient lines of an elite bread wheat variety

    Science.gov (United States)

    Five isogenic wheat lines deficient in high-molecular weight subunit (HMW-GS) proteins encoded by the B-genome were identified from a fast-neutron radiation-mutagenized population of Summit, an elite variety of bread wheat (Triticum aestivum L.). The mutant lines differ from the wild-type progenit...

  10. Survey of magnetospheric line radiation events observed by the DEMETER spacecraft

    Czech Academy of Sciences Publication Activity Database

    Němec, František; Parrot, M.; Santolík, Ondřej; Rodger, C. J.; Rycroft, M. J.; Hayosh, Mykhaylo; Shklyar, D.; Demekhov, A.

    2009-01-01

    Roč. 114, May 7 (2009), A05203/1-A05203/11 ISSN 0148-0227 Institutional research plan: CEZ:AV0Z30420517 Keywords : Magnetospheric Line Radiation * MLR * DEMETER Subject RIV: BL - Plasma and Gas Discharge Physics Impact factor: 3.082, year: 2009 http://onlinelibrary.wiley.com/doi/10.1029/2008JA014016/abstract

  11. Radiation Response in Two HPV-Infected Head-and-Neck Cancer Cell Lines in Comparison to a Non-HPV-Infected Cell Line and Relationship to Signaling Through AKT

    International Nuclear Information System (INIS)

    Gupta, Anjali K.; Lee, John H.; Wilke, Werner W.; Quon, Harry; Smith, Gareth; Maity, Amit; Buatti, John M.; Spitz, Douglas R.

    2009-01-01

    Purpose: Human papilloma virus (HPV)-associated cancers of the head and neck (H and N) are increasing in frequency and are often treated with radiation. There are conflicting data in the literature regarding the radiation response in the presence of HPV infection, with some data suggesting they may be more sensitive to radiation. There are few studies looking at in vitro effects of HPV and further sensitization by inhibitors of specific signaling pathways. We are in the process of starting a clinical trial in H and N cancer patients using nelfinavir (NFV) (which inhibits Akt) and it would be important to know the effect of HPV on radiation response ± NFV. Methods and Materials: Two naturally infected HPV-16 cell lines (UPCI-SCC90 and UMSCC47) and the HPV-negative SQ20B H and N squamous carcinoma cells were used. Western blots with or without 10 uM NFV were done to evaluate signaling from the PI3K-Akt pathway. Clonogenic assays were done in the three cell lines with or without NFV. Results: Both UPCI-SCC90 and UMSCC47 cells were sensitive to radiation as compared with SQ20B and the degree corresponded to Akt activation. The SQ20B cell line has an activating mutation in EGFR resulting in phosphorylation (P) of Akt; UMSCC47 has decreased P-phosphatase and TENsin (PTEN), resulting in increased P-Akt; UPCI-SCC90 had overexpression of P-PTEN and decreased P-Akt. NFV resulted in downregulation of Akt in all three cell lines, resulting in sensitization to radiation. Conclusions: HPV-infected H and N cancers are sensitive to radiation. The degree of sensitivity correlates to Akt activation and they can be further sensitized by NFV.

  12. A hybrid transport-diffusion Monte Carlo method for frequency-dependent radiative-transfer simulations

    International Nuclear Information System (INIS)

    Densmore, Jeffery D.; Thompson, Kelly G.; Urbatsch, Todd J.

    2012-01-01

    Discrete Diffusion Monte Carlo (DDMC) is a technique for increasing the efficiency of Implicit Monte Carlo radiative-transfer simulations in optically thick media. In DDMC, particles take discrete steps between spatial cells according to a discretized diffusion equation. Each discrete step replaces many smaller Monte Carlo steps, thus improving the efficiency of the simulation. In this paper, we present an extension of DDMC for frequency-dependent radiative transfer. We base our new DDMC method on a frequency-integrated diffusion equation for frequencies below a specified threshold, as optical thickness is typically a decreasing function of frequency. Above this threshold we employ standard Monte Carlo, which results in a hybrid transport-diffusion scheme. With a set of frequency-dependent test problems, we confirm the accuracy and increased efficiency of our new DDMC method.

  13. The cloud radiative feedback of a midlatitude squall line system and implication for climate study

    International Nuclear Information System (INIS)

    Chin, H.N.S.

    1992-01-01

    The main objectives of this study are (1) to study the impact of longwave and shortwave radiation on the thermodynamic and kinematic structure of a midlatitude squall line; and (2) to explore the influence of specifically including the ice phase in the cloud-radiation feedback mechanism for climate models

  14. Chromosomal mutations and chromosome loss measured in a new human-hamster hybrid cell line, ALC: studies with colcemid, ultraviolet irradiation, and 137Cs gamma-rays

    Science.gov (United States)

    Kraemer, S. M.; Waldren, C. A.; Chatterjee, A. (Principal Investigator)

    1997-01-01

    Small mutations, megabase deletions, and aneuploidy are involved in carcinogenesis and genetic defects, so it is important to be able to quantify these mutations and understand mechanisms of their creation. We have previously quantified a spectrum of mutations, including megabase deletions, in human chromosome 11, the sole human chromosome in a hamster-human hybrid cell line AL. S1- mutants have lost expression of a human cell surface antigen, S1, which is encoded by the M1C1 gene at 11p13 so that mutants can be detected via a complement-mediated cytotoxicity assay in which S1+ cells are killed and S1- cells survive. But loss of genes located on the tip of the short arm of 11 (11p15.5) is lethal to the AL hybrid, so that mutants that have lost the entire chromosome 11 die and escape detection. To circumvent this, we fused AL with Chinese hamster ovary (CHO) cells to produce a new hybrid, ALC, in which the requirement for maintaining 11p15.5 is relieved, allowing us to detect mutations events involving loss of 11p15.5. We evaluated the usefulness of this hybrid by conducting mutagenesis studies with colcemid, 137Cs gamma-radiation and UV 254 nm light. Colcemid induced 1000 more S1- mutants per unit dose in ALC than in AL; the increase for UV 254 nm light was only two-fold; and the increase for 137Cs gamma-rays was 12-fold. The increase in S1- mutant fraction in ALC cells treated with colcemid and 137Cs gamma-rays were largely due to chromosome loss and 11p deletions often containing a breakpoint within the centromeric region.

  15. Uncertainty characterization of particle depth measurement using digital in-line holography and the hybrid method.

    Science.gov (United States)

    Gao, Jian; Guildenbecher, Daniel R; Reu, Phillip L; Chen, Jun

    2013-11-04

    In the detection of particles using digital in-line holography, measurement accuracy is substantially influenced by the hologram processing method. In particular, a number of methods have been proposed to determine the out-of-plane particle depth (z location). However, due to the lack of consistent uncertainty characterization, it has been unclear which method is best suited to a given measurement problem. In this work, depth determination accuracies of seven particle detection methods, including a recently proposed hybrid method, are systematically investigated in terms of relative depth measurement errors and uncertainties. Both synthetic and experimental holograms of particle fields are considered at conditions relevant to particle sizing and tracking. While all methods display a range of particle conditions where they are most accurate, in general the hybrid method is shown to be the most robust with depth uncertainty less than twice the particle diameter over a wide range of particle field conditions.

  16. Effects of radiation and porphyrin on mitosis and chromosomes in human hematopoietic cell lines

    International Nuclear Information System (INIS)

    Tan, J.C.; Huang, C.C.; Fiel, R.J.

    1976-01-01

    The effect on mitosis of a human hematopoietic cell line RPMI-1788 treated with a metal chelate (Zn ++ ) of meso-tetra (p-carboxyphenyl) porphine (Zn-TCPP) alone at various concentrations or in combination with gamma-irradiation at various doses were studied. The results showed that both Zn-TCPP and radiation were effective in interfering with normal mitosis and that the effect of radiation was relatively more effective. Data also suggest interacting effects between Zn-TCPP and gamma-irradiation. At low doses of radiation, Zn-TCPP potentiated the effect of radiation. The reverse seemed to be true at a high dose of radiation. The effects of two porphyrins (Zn-TCPP and hematoporphyrin) and radiation on chromosomes were also studied. Chromosomal aberrations characteristic of radiation were observed. The porphyrins were found not to be effective chromosome-breaking agents under the experimental conditions tested

  17. Fast and Accurate Hybrid Stream PCRTMSOLAR Radiative Transfer Model for Reflected Solar Spectrum Simulation in the Cloudy Atmosphere

    Science.gov (United States)

    Yang, Qiguang; Liu, Xu; Wu, Wan; Kizer, Susan; Baize, Rosemary R.

    2016-01-01

    A hybrid stream PCRTM-SOLAR model has been proposed for fast and accurate radiative transfer simulation. It calculates the reflected solar (RS) radiances with a fast coarse way and then, with the help of a pre-saved matrix, transforms the results to obtain the desired high accurate RS spectrum. The methodology has been demonstrated with the hybrid stream discrete ordinate (HSDO) radiative transfer (RT) model. The HSDO method calculates the monochromatic radiances using a 4-stream discrete ordinate method, where only a small number of monochromatic radiances are simulated with both 4-stream and a larger N-stream (N = 16) discrete ordinate RT algorithm. The accuracy of the obtained channel radiance is comparable to the result from N-stream moderate resolution atmospheric transmission version 5 (MODTRAN5). The root-mean-square errors are usually less than 5x10(exp -4) mW/sq cm/sr/cm. The computational speed is three to four-orders of magnitude faster than the medium speed correlated-k option MODTRAN5. This method is very efficient to simulate thousands of RS spectra under multi-layer clouds/aerosols and solar radiation conditions for climate change study and numerical weather prediction applications.

  18. Achievements in NS rapeseed hybrids breeding

    Directory of Open Access Journals (Sweden)

    Marjanović-Jeromela Ana

    2016-01-01

    Full Text Available The increased production of oilseed rape (Brassica napus L. is evident on a global scale, but also in Serbia in the last decade. Rapeseed is used primarily for vegetable oil and processing industry, but also as a source of protein for animal feed and green manure. Following the cultivation of varieties, breeding and cultivation of hybrid rapeseed started in the 1990's, to take advantage of heterosis in F1 generation, while protecting the breeder's rights during seed commercialization. The breeding of hybrid oilseed rape requires high quality starting material (lines with good combining abilities for introduction of male sterility. Ogura sterility system is primarily used at the Institute of Field and Vegetable Crops, Novi Sad, Serbia. To use this system, separate lines are modified with genes for cytoplasmic male sterility (cms female line - mother line and restoration of fertility (Rf male lines - father line. In order to maintain the sterility of the mother line it is necessary to produce a maintainer line of cytoplasmic male sterility. Creation of these lines and hybrids at the Institute of Field and Vegetable Crops was successfully monitored with intense use of cytogenetic laboratory methods. The structure and vitality of pollen, including different phases during meiosis were checked so that cms stability was confirmed during the introduction of these genes into different lines. Rapeseed breeding program in Serbia resulted in numerous varieties through collaboration of researchers engaged in breeding and genetics of this plant species. So far, in addition to 12 varieties of winter rapeseed and two varieties of spring rapeseed, a new hybrid of winter rapeseed NS Ras was registered in Serbia. NS Ras is an early-maturing hybrid characterized by high seed yield and oil content. Average yield of NS Ras for two seasons and three sites was 4256 kg ha-1 of seed and 1704 kg ha-1 of oil. Three promising winter rapeseed hybrids are in the process of

  19. Towards integration of PET/MR hybrid imaging into radiation therapy treatment planning

    International Nuclear Information System (INIS)

    Paulus, Daniel H.; Thorwath, Daniela; Schmidt, Holger; Quick, Harald H.

    2014-01-01

    Purpose: Multimodality imaging has become an important adjunct of state-of-the-art radiation therapy (RT) treatment planning. Recently, simultaneous PET/MR hybrid imaging has become clinically available and may also contribute to target volume delineation and biological individualization in RT planning. For integration of PET/MR hybrid imaging into RT treatment planning, compatible dedicated RT devices are required for accurate patient positioning. In this study, prototype RT positioning devices intended for PET/MR hybrid imaging are introduced and tested toward PET/MR compatibility and image quality. Methods: A prototype flat RT table overlay and two radiofrequency (RF) coil holders that each fix one flexible body matrix RF coil for RT head/neck imaging have been evaluated within this study. MR image quality with the RT head setup was compared to the actual PET/MR setup with a dedicated head RF coil. PET photon attenuation and CT-based attenuation correction (AC) of the hardware components has been quantitatively evaluated by phantom scans. Clinical application of the new RT setup in PET/MR imaging was evaluated in anin vivo study. Results: The RT table overlay and RF coil holders are fully PET/MR compatible. MR phantom and volunteer imaging with the RT head setup revealed high image quality, comparable to images acquired with the dedicated PET/MR head RF coil, albeit with 25% reduced SNR. Repositioning accuracy of the RF coil holders was below 1 mm. PET photon attenuation of the RT table overlay was calculated to be 3.8% and 13.8% for the RF coil holders. With CT-based AC of the devices, the underestimation error was reduced to 0.6% and 0.8%, respectively. Comparable results were found within the patient study. Conclusions: The newly designed RT devices for hybrid PET/MR imaging are PET and MR compatible. The mechanically rigid design and the reproducible positioning allow for straightforward CT-based AC. The systematic evaluation within this study provides the

  20. Synchrotron radiation-based quasi-elastic scattering using time-domain interferometry with multi-line gamma rays.

    Science.gov (United States)

    Saito, Makina; Masuda, Ryo; Yoda, Yoshitaka; Seto, Makoto

    2017-10-02

    We developed a multi-line time-domain interferometry (TDI) system using 14.4 keV Mössbauer gamma rays with natural energy widths of 4.66 neV from 57 Fe nuclei excited using synchrotron radiation. Electron density fluctuations can be detected at unique lengths ranging from 0.1 nm to a few nm on time scales from several nanoseconds to the sub-microsecond order by quasi-elastic gamma-ray scattering (QGS) experiments using multi-line TDI. In this report, we generalize the established expression for a time spectrum measured using an identical single-line gamma-ray emitter pair to the case of a nonidentical pair of multi-line gamma-ray emitters by considering the finite energy width of the incident synchrotron radiation. The expression obtained illustrates the unique characteristics of multi-line TDI systems, where the finite incident energy width and use of a nonidentical emitter pair produces further information on faster sub-picosecond-scale dynamics in addition to the nanosecond dynamics; this was demonstrated experimentally. A normalized intermediate scattering function was extracted from the spectrum and its relaxation form was determined for a relaxation time of the order of 1 μs, even for relatively large momentum transfer of ~31 nm -1 . The multi-line TDI method produces a microscopic relaxation picture more rapidly and accurately than conventional single-line TDI.

  1. Development of new on line statistical program for the Korean society for radiation oncology

    International Nuclear Information System (INIS)

    Song, Si Yeol; Ahn, Seung Do; Choi, Eun Kyung; Chung, Weon Kuu; Shin, Kyung Hwan; Cho, Kwan Ho

    2015-01-01

    To develop new on-line statistical program for the Korean Society for Radiation Oncology (KOSRO) to collect and extract medical data in radiation oncology more efficiently. The statistical program is a web-based program. The directory was placed in a sub-folder of the homepage of KOSRO and its web address is http://www.kosro.or.kr/asda. The operating systems server is Linux and the webserver is the Apache HTTP server. For database (DB) server, MySQL is adopted and dedicated scripting language is the PHP. Each ID and password are controlled independently and all screen pages for data input or analysis are made to be friendly to users. Scroll-down menu is actively used for the convenience of user and the consistence of data analysis. Year of data is one of top categories and main topics include human resource, equipment, clinical statistics, specialized treatment and research achievement. Each topic or category has several subcategorized topics. Real-time on-line report of analysis is produced immediately after entering each data and the administrator is able to monitor status of data input of each hospital. Backup of data as spread sheets can be accessed by the administrator and be used for academic works by any members of the KOSRO. The new on-line statistical program was developed to collect data from nationwide departments of radiation oncology. Intuitive screen and consistent input structure are expected to promote entering data of member hospitals and annual statistics should be a cornerstone of advance in radiation oncology

  2. Development of new on-line statistical program for the Korean Society for Radiation Oncology.

    Science.gov (United States)

    Song, Si Yeol; Ahn, Seung Do; Chung, Weon Kuu; Shin, Kyung Hwan; Choi, Eun Kyung; Cho, Kwan Ho

    2015-06-01

    To develop new on-line statistical program for the Korean Society for Radiation Oncology (KOSRO) to collect and extract medical data in radiation oncology more efficiently. The statistical program is a web-based program. The directory was placed in a sub-folder of the homepage of KOSRO and its web address is http://www.kosro.or.kr/asda. The operating systems server is Linux and the webserver is the Apache HTTP server. For database (DB) server, MySQL is adopted and dedicated scripting language is the PHP. Each ID and password are controlled independently and all screen pages for data input or analysis are made to be friendly to users. Scroll-down menu is actively used for the convenience of user and the consistence of data analysis. Year of data is one of top categories and main topics include human resource, equipment, clinical statistics, specialized treatment and research achievement. Each topic or category has several subcategorized topics. Real-time on-line report of analysis is produced immediately after entering each data and the administrator is able to monitor status of data input of each hospital. Backup of data as spread sheets can be accessed by the administrator and be used for academic works by any members of the KOSRO. The new on-line statistical program was developed to collect data from nationwide departments of radiation oncology. Intuitive screen and consistent input structure are expected to promote entering data of member hospitals and annual statistics should be a cornerstone of advance in radiation oncology.

  3. Development of new on line statistical program for the Korean society for radiation oncology

    Energy Technology Data Exchange (ETDEWEB)

    Song, Si Yeol; Ahn, Seung Do; Choi, Eun Kyung [Dept. of Radiation Oncology, Asan Medical Center, University of Ulsan College of Medicine, Seoul (Korea, Republic of); Chung, Weon Kuu [Dept. of Radiation Oncology, Kyung Hee University Hospital at Kangdong, Kyung Hee University School of Medicine, Seoul (Korea, Republic of); Shin, Kyung Hwan [Dept. of Radiation Oncology, Seoul National University Hospital, Seoul National University College of Medicine, Seoul (Korea, Republic of); Cho, Kwan Ho [Dept. of Radiation Oncology, Proton Therapy Center, National Cancer Center Hospital, Goyang (Korea, Republic of)

    2015-06-15

    To develop new on-line statistical program for the Korean Society for Radiation Oncology (KOSRO) to collect and extract medical data in radiation oncology more efficiently. The statistical program is a web-based program. The directory was placed in a sub-folder of the homepage of KOSRO and its web address is http://www.kosro.or.kr/asda. The operating systems server is Linux and the webserver is the Apache HTTP server. For database (DB) server, MySQL is adopted and dedicated scripting language is the PHP. Each ID and password are controlled independently and all screen pages for data input or analysis are made to be friendly to users. Scroll-down menu is actively used for the convenience of user and the consistence of data analysis. Year of data is one of top categories and main topics include human resource, equipment, clinical statistics, specialized treatment and research achievement. Each topic or category has several subcategorized topics. Real-time on-line report of analysis is produced immediately after entering each data and the administrator is able to monitor status of data input of each hospital. Backup of data as spread sheets can be accessed by the administrator and be used for academic works by any members of the KOSRO. The new on-line statistical program was developed to collect data from nationwide departments of radiation oncology. Intuitive screen and consistent input structure are expected to promote entering data of member hospitals and annual statistics should be a cornerstone of advance in radiation oncology.

  4. POTENTIAL OF COMMERCIAL MAIZE HYBRIDS TO GENERATE INBRED LINES IN BREEDING PROGRAMS POTENCIAL DE HÍBRIDOS COMERCIAIS DE MILHO PARA OBTENÇÃO DE LINHAGENS EM PROGRAMAS DE MELHORAMENTO

    Directory of Open Access Journals (Sweden)

    Maria Elisa Ayres Guidetti Zagatto Paterniani

    2010-08-01

    Full Text Available

    line-height: 120%; text-decoration: none;" lang="pt-BR" align="JUSTIFY">With the objective of identifying the best commercial hybrids to extract maize lines, a top cross scheme of 49 endogamic partial lines (S3 was carried out with the IA33 tester. The resultant hybrids were evaluated for grain yield, in Campinas, Mococa, and Palmital (São Paulo State, Brazil, in two agricultural years (2005/2006 and 2006/2007, in a randomized block design, with three replications, in two experiments (TC1 and TC2. Individual and group variance analysis and a grouped analysis of the experiments with common treatments were carried out, for each place. The top cross hybrids were grouped according to the line origins. Afterwards, orthogonal contrasts were carried out by using the Student’s t test, in order to compare the hybrid groups. It was verified that the use of commercial hybrids to extract lines is an interesting strategy. In general, it was also verified that the top cross hybrid groups, whose lines originated from the AG1051, Master, and XL357 hybrids, obtained high yield, with a higher potential for lines extraction.

    line-height: 120%; text-decoration: none;" lang="en-US" align="JUSTIFY">KEY-WORDS: Zea mays L.; top cross hybrids; partially endogamic lines (S3.

    line-height: 120%; text-decoration: none;" lang="pt-BR" align="JUSTIFY">O presente trabalho teve por objetivo identificar os melhores híbridos comerciais para extração de linhagens de milho. Para isto, foram

  5. Solving radiative transfer with line overlaps using Gauss-Seidel algorithms

    Science.gov (United States)

    Daniel, F.; Cernicharo, J.

    2008-09-01

    Context: The improvement in observational facilities requires refining the modelling of the geometrical structures of astrophysical objects. Nevertheless, for complex problems such as line overlap in molecules showing hyperfine structure, a detailed analysis still requires a large amount of computing time and thus, misinterpretation cannot be dismissed due to an undersampling of the whole space of parameters. Aims: We extend the discussion of the implementation of the Gauss-Seidel algorithm in spherical geometry and include the case of hyperfine line overlap. Methods: We first review the basics of the short characteristics method that is used to solve the radiative transfer equations. Details are given on the determination of the Lambda operator in spherical geometry. The Gauss-Seidel algorithm is then described and, by analogy to the plan-parallel case, we see how to introduce it in spherical geometry. Doing so requires some approximations in order to keep the algorithm competitive. Finally, line overlap effects are included. Results: The convergence speed of the algorithm is compared to the usual Jacobi iterative schemes. The gain in the number of iterations is typically factors of 2 and 4 for the two implementations made of the Gauss-Seidel algorithm. This is obtained despite the introduction of approximations in the algorithm. A comparison of results obtained with and without line overlaps for N2H^+, HCN, and HNC shows that the J=3-2 line intensities are significantly underestimated in models where line overlap is neglected.

  6. Transfer of HMW glutenin subunits from Aegilops kotschyi to wheat through radiation hybridization

    International Nuclear Information System (INIS)

    Singh, Jasmeet; Sheikh, Imran; Sharma, Prachi

    2016-01-01

    High molecular weight glutenin subunits (HMWGS) are responsible for dough elasticity and bread making quality of bread wheat. Related wild non-progenitor species, Aegilops kotschyi possesses higher molecular weight x and y glutenin subunits than the bread wheat cultivars. A wheat-Aegilops substitution line with 1U chromosome was used for the transfer of (HMWGS) of 1U to wheat by using pollen radiation hybridization approach. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis profiling showed different patterns of allelic variations with either the presence or absence of HMWGS, Glu-1A (1, null), Glu-1B (7, 7 + 8, 17 + 18) and Glu-1D (5 + 10, 2 + 12, null). The pollen irradiated wheat-Aegilops derivatives, B-56-1-4-2, B-56-1-4-3, B-14-1 and B-14-2 with Glu1Ux and 1Uy and absence or presence of some Glu-1A and Glu-1B HMWGS showed high micro SDS sedimentation test (MST) values while B-16-1 and B-16-2 had moderate MST values and high protein content. However, B-58-3 with transfer of Glu-1Ux + 1Uy for Glu-1D showed very low MST values indicating that Glu-1Ux + 1Uy enhance MST value only in the presence of Glu1D HMWGS. The transfer/substitution of alien HMW-GS for Glu-1A and or Glu-1B loci only can lead to improved bread making quality of wheat. (author)

  7. Line-driven disk winds in active galactic nuclei: The critical importance of ionization and radiative transfer

    Energy Technology Data Exchange (ETDEWEB)

    Higginbottom, Nick; Knigge, Christian; Matthews, James H. [School of Physics and Astronomy, University of Southampton, Highfield, Southampton, SO17 1BJ (United Kingdom); Proga, Daniel [Department of Physics and Astronomy, University of Nevada, Las Vegas, 4505 South Maryland Parkway, Las Vegas, NV 89154-4002 (United States); Long, Knox S. [Space Telescope Science Institute, 3700 San Martin Drive, Baltimore, MD 21218 (United States); Sim, Stuart A., E-mail: nick_higginbottom@fastmail.fm [School of Mathematics and Physics, Queens University Belfast, University Road, Belfast, BT7 1NN (United Kingdom)

    2014-07-01

    Accretion disk winds are thought to produce many of the characteristic features seen in the spectra of active galactic nuclei (AGNs) and quasi-stellar objects (QSOs). These outflows also represent a natural form of feedback between the central supermassive black hole and its host galaxy. The mechanism for driving this mass loss remains unknown, although radiation pressure mediated by spectral lines is a leading candidate. Here, we calculate the ionization state of, and emergent spectra for, the hydrodynamic simulation of a line-driven disk wind previously presented by Proga and Kallman. To achieve this, we carry out a comprehensive Monte Carlo simulation of the radiative transfer through, and energy exchange within, the predicted outflow. We find that the wind is much more ionized than originally estimated. This is in part because it is much more difficult to shield any wind regions effectively when the outflow itself is allowed to reprocess and redirect ionizing photons. As a result, the calculated spectrum that would be observed from this particular outflow solution would not contain the ultraviolet spectral lines that are observed in many AGN/QSOs. Furthermore, the wind is so highly ionized that line driving would not actually be efficient. This does not necessarily mean that line-driven winds are not viable. However, our work does illustrate that in order to arrive at a self-consistent model of line-driven disk winds in AGN/QSO, it will be critical to include a more detailed treatment of radiative transfer and ionization in the next generation of hydrodynamic simulations.

  8. Dose rate effect models for biological reaction to ionizing radiation in human cell lines

    International Nuclear Information System (INIS)

    Magae, Junji; Ogata, Hiromitsu

    2008-01-01

    Full text: Because of biological responses to ionizing radiation are dependent on irradiation time or dose rate as well as dose, simultaneous inclusion of dose and dose rate is required to evaluate the risk of long term irradiation at low dose rates. We previously published a novel statistical model for dose rate effect, modified exponential (MOE) model, which predicts irradiation time-dependent biological response to low dose rate ionizing radiation, by analyzing micronucleus formation and growth inhibition in a human osteosarcoma cell line, exposed to wide range of doses and dose rates of gamma-rays. MOE model demonstrates that logarithm of median effective dose exponentially increases in low dose rates, and thus suggests that the risk approaches to zero at infinitely low dose rate. In this paper, we extend the analysis in various kinds of human cell lines exposed to ionizing radiation for more than a year. We measured micronucleus formation and [ 3 H]thymidine uptake in human cell lines including an osteosarcoma, a DNA-dependent protein kinase-deficient glioma, a SV40-transformed fibroblast derived from an ataxia telangiectasia patient, a normal fibroblast, and leukemia cell lines. Cells were exposed to gamma-rays in irradiation room bearing 50,000 Ci of cobalt-60. After the irradiation, they were cultured for 24 h in the presence of cytochalasin B to block cytokinesis, and cytoplasm and nucleus were stained with DAPI and prospidium iodide. The number of binuclear cells bearing a micronucleus was counted under a fluorescence microscope. For proliferation inhibition, cells were cultured for 48 h after the irradiation and [ 3 H] thymidine was pulsed for 4 h before harvesting. We statistically analyzed the data for quantitative evaluation of radiation risk. While dose and dose rate relationship cultured within one month followed MOE model in cell lines holding wild-type DNA repair system, dose rate effect was greatly impaired in DNA repair-deficient cell lines

  9. Construction of a river buffalo (Bubalus bubalis whole-genome radiation hybrid panel and preliminary RH mapping of chromosomes 3 and 10

    Directory of Open Access Journals (Sweden)

    J.E. Womack

    2010-02-01

    Full Text Available The buffalo (Bubalus bubalis not only is a useful source of milk, it also provides meat and works as a natural source of labor and biogas. To establish a project for buffalo genome mapping a 5,000-rad whole genome radiation hybrid panel was constructed for river buffalo and used to build preliminary RH maps from two chromosomes (BBU 3 and BBU10. The preliminary maps contain 66 markers, including coding genes, cattle ESTs and microsatellite loci. The RH maps presented here are the starting point for mapping additional loci, in particular, genes and expressed sequence tags that will allow detailed comparative maps between buffalo, cattle and other species to be constructed. A large quantity of DNA has been prepared from the cell lines forming the RH panel reported here and will be made publicly available to the international community both for the study of chromosome evolution and for the improvement of traits important to the role of buffalo in animal agriculture.

  10. Fruit extract from a Sechium edule hybrid induce apoptosis in leukaemic cell lines but not in normal cells.

    Science.gov (United States)

    Aguiñiga-Sánchez, Itzen; Soto-Hernández, Marcos; Cadena-Iñiguez, Jorge; Ruíz-Posadas, Lucero del Mar; Cadena-Zamudio, Jorge David; González-Ugarte, Ana Karen; Steider, Benny Weiss; Santiago-Osorio, Edelmiro

    2015-01-01

    The antiproliferative potential of a crude extract from the chayote hybrid H-837-07-GISeM® and its potential for apoptosis induction were assessed in leukaemic cell lines and normal mouse bone marrow mononuclear cells (BM-MNCs). The extract strongly inhibited the proliferation of the P388, J774, and WEHI-3 cell lines (with an IC50 below 1.3 μg·mL(-1)), reduced cell viability, and induced apoptotic body production, phosphatidylserine translocation, and DNA fragmentation. However, the extract had no effect on BM-MNCs. We postulate that these properties make the extract a good candidate for an anti-tumour agent for clinical use.

  11. Computer controlled vacuum control system for synchrotron radiation beam lines

    International Nuclear Information System (INIS)

    Goldberg, S.M.; Wang, C.; Yang, J.

    1983-01-01

    The increasing number and complexity of vacuum control systems at the Stanford Synchrotron Radiation Laboratory has resulted in the need to computerize its operations in order to lower costs and increase efficiency of operation. Status signals are transmitted through digital and analog serial data links which use microprocessors to monitor vacuum status continuously. Each microprocessor has a unique address and up to 256 can be connected to the host computer over a single RS232 data line. A FORTRAN program on the host computer will request status messages and send control messages via only one RS232 line per beam line, signal the operator when a fault condition occurs, take automatic corrective actions, warn of impending valve failure, and keep a running log of all changes in vacuum status for later recall. Wiring costs are thus greatly reduced and more status conditions can be monitored without adding excessively to the complexity of the system. Operators can then obtain status reports at various locations in the lab quickly without having to read a large number of meter and LED's

  12. Line radiation of multicharged ions with the Fermi-Dirac level distribution of electrons at high temperatures

    International Nuclear Information System (INIS)

    Garanin, S.F.

    2003-01-01

    Line radiation of multicharged ions with the Fermi-Dirac electron distribution by levels in the range of plasma temperatures, when electron movement may be considered quasiclassical, while potential, in which they move, is the Coulomb one, is considered. The spectrum and intensity of ion radiation are calculated. Within high plasma densities the radiation intensity per one ion proved to be independent of density and proportional to T 2 [ru

  13. A comparison of the levels of oil, carotenoids, and lipolytic enzyme activities in modern lines and hybrids of grain sorghum

    Science.gov (United States)

    Thirteen modern commercial grain sorghum hybrids and five sorghum lines were extracted and the levels of oil and carotenoids were analyzed and compared. The same samples were also evaluated for lipolytic enzyme activity. The oil content in all eighteen samples ranged from 3.21 to 4.29 wt%. Beta c...

  14. In vitro radiation and chemotherapy sensitivity of established cell lines of human small cell lung cancer and its large cell morphological variants

    International Nuclear Information System (INIS)

    Carney, D.N.; Mitchell, J.B.; Kinsella, T.J.

    1983-01-01

    The in vitro response to radiation and chemotherapeutic drugs of cell lines established from 7 patients with small cell (SC) lung cancer were tested using a soft agarose clonogenic assay. Five cell lines retained the typical morphological and biochemical amine precursor uptake decarboxylation characteristics of SC, while two cell lines had undergone ''transformation'' to large cell (LC) morphological variants with loss of amine precursor uptake decarboxylation cell characteristics of SC. The radiation survival curves for the SC lines were characterized by D0 values ranging from 51 to 140 rads and extrapolation values (n) ranging from 1.0 to 3.3. While the D0 values of the radiation survival curves of the LC variants were similar (91 and 80 rads), the extrapolation values were 5.6 and 11.1 In vitro chemosensitivity testing of the cell lines revealed an excellent correlation between prior treatment status of the patient and in vitro sensitivity or resistance. No correlation was observed between in vitro chemosensitivity and radiation response. These data suggest that transformation of SC to LC with loss of amine precursor uptake and decarboxylation characteristics is associated with a marked increase in radiation resistance (n) in vitro. The observation of a 2- to 5-fold increase in survival of the LC compared to the SC lines following 200 rads suggests that the use of larger daily radiation fractions and/or radiation-sensitizing drugs might lead to a significantly greater clinical response in patients with LC morphology. This clinical approach may have a major impact on patient response and survival

  15. RADIATIVE TRANSFER MODELING OF THE ENIGMATIC SCATTERING POLARIZATION IN THE SOLAR Na i D{sub 1} LINE

    Energy Technology Data Exchange (ETDEWEB)

    Belluzzi, Luca [Istituto Ricerche Solari Locarno, CH-6605 Locarno Monti (Switzerland); Bueno, Javier Trujillo [Instituto de Astrofísica de Canarias, E-38205 La Laguna, Tenerife (Spain); Degl’Innocenti, Egidio Landi [Dipartimento di Fisica e Astronomia, Università di Firenze, I-50125 Firenze (Italy)

    2015-12-01

    The modeling of the peculiar scattering polarization signals observed in some diagnostically important solar resonance lines requires the consideration of the detailed spectral structure of the incident radiation field as well as the possibility of ground level polarization, along with the atom's hyperfine structure and quantum interference between hyperfine F-levels pertaining either to the same fine structure J-level, or to different J-levels of the same term. Here we present a theoretical and numerical approach suitable for solving this complex non-LTE radiative transfer problem. This approach is based on the density-matrix metalevel theory (where each level is viewed as a continuous distribution of sublevels) and on accurate formal solvers of the transfer equations and efficient iterative methods. We show an application to the D-lines of Na i, with emphasis on the enigmatic D{sub 1} line, pointing out the observable signatures of the various physical mechanisms considered. We demonstrate that the linear polarization observed in the core of the D{sub 1} line may be explained by the effect that one gets when the detailed spectral structure of the anisotropic radiation responsible for the optical pumping is taken into account. This physical ingredient is capable of introducing significant scattering polarization in the core of the Na i D{sub 1} line without the need for ground-level polarization.

  16. Design of Underwater Robot Lines Based on a Hybrid Automatic Optimization Strategy

    Institute of Scientific and Technical Information of China (English)

    Wenjing Lyu; Weilin Luo

    2014-01-01

    In this paper, a hybrid automatic optimization strategy is proposed for the design of underwater robot lines. Isight is introduced as an integration platform. The construction of this platform is based on the user programming and several commercial software including UG6.0, GAMBIT2.4.6 and FLUENT12.0. An intelligent parameter optimization method, the particle swarm optimization, is incorporated into the platform. To verify the strategy proposed, a simulation is conducted on the underwater robot model 5470, which originates from the DTRC SUBOFF project. With the automatic optimization platform, the minimal resistance is taken as the optimization goal;the wet surface area as the constraint condition; the length of the fore-body, maximum body radius and after-body’s minimum radius as the design variables. With the CFD calculation, the RANS equations and the standard turbulence model are used for direct numerical simulation. By analyses of the simulation results, it is concluded that the platform is of high efficiency and feasibility. Through the platform, a variety of schemes for the design of the lines are generated and the optimal solution is achieved. The combination of the intelligent optimization algorithm and the numerical simulation ensures a global optimal solution and improves the efficiency of the searching solutions.

  17. Study of the heat transfers spectral radiation - conduction - natural convection in hybrid photovoltaic systems for buildings

    International Nuclear Information System (INIS)

    Muresan, C.

    2005-01-01

    The present work is supported by the CSTB and the ADEME and is a part of an Integrated Research Project - Energy Program of CNRS - (http://www.imp.cnrs.fr/energie/) coordinated by the CETHIL: 'Integration of hybrid Thermal - Photovoltaic solar collector in buildings'. In this context, this thesis represents upstream studies led in the I.R.P., pursuing the study itself of these hybrid components in stage of integration to the framework of buildings (thermal/electric management in response to the needs). Its objective falls under an action to identify and look further into knowledge of the limiting factors of the efficiency of these hybrid components (the operating temperature of the photosensitive cells), to identify the enduring scientific bolts persisting and to contribute to removing them. To reach this aim, predictive numerical tools are developed in order to guide and follow the future evolutions of these active wall elements. The problems related to the Photovoltaic components of Mono or Poly crystalline type, namely the risk of heating of the modules included within the built framework that can lead to a degradation of their energy efficiency. The developed model aims at obtaining the evaluation of the internal field of temperature. The modeling of the radiative behavior of multi-layer components of not scattering semi-transparent media is carried out. Propagation of a collimated flux corresponds to the direct solar radiation, and a diffuse flux represents the solar radiation diffused and that resulting from the other external sources (environment). Both are treated in a separate way. The incidental radiation power and radiative net flux are thus evaluated by a superposition of the values obtained at the time of the separate studies of the two components. The collimated component of incidental flux is treated according to an approach of 'ray tracing' type. The Discrete Ordinates Method (DOM) associated to the method of finite volumes, is employed for the

  18. Effect of recombinant adenovirus encoding human p53 tumor suppressor gene combined with radiation therapy on human lymphoma cells lines

    International Nuclear Information System (INIS)

    Yu Zeyang; Fan Wo; Li Dongqing; Zhu Ran; Wan Jianmei; Wang Yongqing; Wu Jinchang

    2008-01-01

    This paper analyzes the inhibitory effect and radiation sensitization of recombinant adenovirus encoding human p53 tumor suppressor gene (rAd-p53) on human lymphoma cell lines. Human lymphoma cell lines were treated with rAd-p53, radiation therapy and combined treatment, respectively. The cell growth inhibition was assessed by MTF. The cell cycle and apoptosis were detected by flow cytometry, and the p53 protein expression was detected by Western blotting. The results showed that extrinsic p53 gene have expressed to some degree, but not at high level. The role of inhibition and radiation sensitivity of rAd-p53 was not significant to human lymphoma cell lines. (authors)

  19. Application of the inter-line PCR for the analyse of genomic rearrangements in radiation-transformed mammalian cell lines; Anwendung der Inter-Line PCR zur Analyse von genomischen Veraenderungen in strahlentransformierten Saeugerzellinien

    Energy Technology Data Exchange (ETDEWEB)

    Leibhard, S.; Smida, J. [Muenchen Univ. (Germany). Strahlenbiologisches Inst.; Eckardt-Schupp, F.; Hieber, L. [GSF-Inst. fuer Strahlenbiologie, Oberschleissheim (Germany)

    1996-12-31

    Repetitive DNA sequences of the LINE-family (long interspersed elements) that are widely distributed among the mammalian genome can be activated or altered by the exposure to ionizing radiation [1]. By the integration at new sites in the genome alterations in the expression of genes that are involved in cell transformation and/or carcinogenesis may occur [2, 3]. A new technique - the inter-LINE PCR - has been developed in order to detect and analyse such genomic rearrangements in radiation-transformed cell lines. From the sites of transformation- or tumour-specific changes in the genome it might be possible to develop new tumour markers for diagnostic purpose. (orig.) [Deutsch] Repetitive DNA-Sequenzen der LINE-Familie, die weit verbreitet im Genom von Saeugerzellen vorkommen, koennen durch Exposition mit ionisierender Strahlung aktiviert und veraendert werden [1]. Durch eine Neu- bzw. Reintegration an anderen Positionen im Genom kann es zu bedeutenden Veraenderungen im Genom der Zelle kommen. Die Expression von Genen, die bei den Prozessen der Zelltransformation bzw. der Karzinogenese beteiligt sind, kann dadurch veraendert werden [2, 3]. Mithilfe der von uns entwickelten Inter-LINE PCR und der anschliessenden Analyse der veraenderten Produktmuster nach gelelektrophoretischer Auftrennung koennen solche `genomic rearrangements` unter Beteiligung von LINE-Elementen untersucht und naeher charakterisiert werden. Durch Klonierung und Sequenzierung transformations- bzw. tumorspezifischer PCR-Produkte sollte es moeglich sein Tumormarker fuer diagnostische Zwecke zu entwickeln. Die Methode wurde fuer die Analyse von Zellen des Syrischen Hamster aufgebaut, sie ist jedoch universell fuer alle Saeuger anwendbar. (orig.)

  20. Relationship between radiation induced activation of DNA repair genes and radiation induced apoptosis in human cell line A431

    International Nuclear Information System (INIS)

    Bom, Hee Seung; Min, Jung Jun; Kim, Kyung Keun; Choi, Keun Hee

    2000-01-01

    The purpose of this study was to evaluate the relationship between radiation-induced acivation of DNA repair genes and radiation induced apoptosis in A431 cell line. Five and 25 Gys of gamma radiation were given to A431 cells by a Cs-137 cell irradiator. Apoptosis was evaluated by flow cytometry using annexin V-fluorescein isothiocyanate and propidium iodide staining. The expression of DNA repair genes was evaluated by both Northern and Western blot analyses. The number of apoptotic cells increased with the increased radiation dose. It increased most significantly at 12 hours after irradiation. Expression of p53, p21, and ℎRAD50 reached the highest level at 12 hours after 5 Gy irradiation. In response to 25 Gy irradiation, ℎRAD50 and p21 were expressed maximally at 12 hours, but p53 and GADD45 genes showed the highest expression level after 12 hours. Induction of apoptosis and DNA repair by ionizing radiation were closely correlated. The peak time of inducing apoptosis and DNA repair was 12 hours in this study model. ℎRAD50, a recently discovered DNA repair gene, was also associated with radiation-induced apoptosis.=20

  1. Investigation of Solar Hybrid Electric/Thermal System with Radiation Concentrator and Thermoelectric Generator

    Directory of Open Access Journals (Sweden)

    Edgar Arturo Chávez Urbiola

    2013-01-01

    Full Text Available An experimental study of a solar-concentrating system based on thermoelectric generators (TEGs was performed. The system included an electrical generating unit with 6 serially connected TEGs using a traditional semiconductor material, Bi2Te3, which was illuminated by concentrated solar radiation on one side and cooled by running water on the other side. A sun-tracking concentrator with a mosaic set of mirrors was used; its orientation towards the sun was achieved with two pairs of radiation sensors, a differential amplifier, and two servomotors. The hot side of the TEGs at midday has a temperature of around 200°C, and the cold side is approximately 50°C. The thermosiphon cooling system was designed to absorb the heat passing through the TEGs and provide optimal working conditions. The system generates 20 W of electrical energy and 200 W of thermal energy stored in water with a temperature of around 50°C. The hybrid system studied can be considered as an alternative to photovoltaic/thermal systems, especially in countries with abundant solar radiation, such as Mexico, China, and India.

  2. Thermal problems on high flux beam lines

    International Nuclear Information System (INIS)

    Avery, R.T.

    1983-09-01

    Wiggler and undulator magnets can provide very intense photon flux densities to beam line components. This paper addresses some thermal/materials consequences due to such impingement. The LBL/Exxon/SSRL hybrid-wiggler Beam Line VI now nearing operation will be able to provide up to approx. 7 kW of total photon power at planned SPEAR operating conditions. The first masks are located at 6.5 meters from the source and may receive a peak power density (transverse to the beam) exceeding 20 kW/cm 2 . Significantly, this heat transfer rate exceeds that radiated from the sun's surface (7 kW/cm 2 ) and is comparable to that of welding torches. Clearing, cooling and configuration are of critical importance. Configurations for the first fixed mask, the movable mask, and the pivot mask on this beam line are presented together with considerations of thermal stress fatigue and of heat transfer by conduction to water-cooling circuits. Some preliminary information on heating of crystals and mirrors is also presented

  3. Integration of on-line imaging, plan adaptation and radiation delivery: proof of concept using digital tomosynthesis

    International Nuclear Information System (INIS)

    Mestrovic, Ante; Otto, Karl; Nichol, Alan; Clark, Brenda G

    2009-01-01

    The main objective of this manuscript is to propose a new approach to on-line adaptive radiation therapy (ART) in which daily image acquisition, plan adaptation and radiation delivery are integrated together and performed concurrently. A method is described in which on-line ART is performed based on intra-fractional digital tomosynthesis (DTS) images. Intra-fractional DTS images were reconstructed as the gantry rotated between treatment positions. An edge detection algorithm was used to automatically segment the DTS images as the gantry arrived at each treatment position. At each treatment position, radiation was delivered based on the treatment plan re-optimized for the most recent DTS image contours. To investigate the feasibility of this method, a model representing a typical prostate, bladder and rectum was used. To simulate prostate deformations, three clinically relevant, non-rigid deformations (small, medium and large) were modeled by systematically deforming the original anatomy. Using our approach to on-line ART, the original treatment plan was successfully adapted to arrive at a clinically acceptable plan for all three non-rigid deformations. In conclusion, we have proposed a new approach to on-line ART in which plan adaptation is performed based on intra-fractional DTS images. The study findings indicate that this approach can be used to re-optimize the original treatment plan to account for non-rigid anatomical deformations. The advantages of this approach are 1) image acquisition and radiation delivery are integrated in a single gantry rotation around the patient, reducing the treatment time, and 2) intra-fractional DTS images can be used to detect and correct for patient motion prior to the delivery of each beam (intra-fractional patient motion).

  4. Study of the cell cycle control for human malignant mesothelioma lines. Interferon and radiations effect

    International Nuclear Information System (INIS)

    Vivo, C.

    1999-01-01

    In order to better understand the inhibition mechanisms of the IFN-R-HU on tumoral development, the IFN-R-U effect on MM lines has been studied. Three groups of lines has been distinguished: eight sensitive lines, two intermediate and three resistant. The sensitive lines showed a triple locking of the cell cycle: in phases S, G1 and G2. The study of the cell cycle control points function, realized by the MM lines radiation exposure showed the points function on G1/S and-or on G2/M and the dependence or non dependence of the cycle stop of the protein P53 and P21 W at F1/CIP1. (A.L.B.)

  5. Adaptive radiation in the Hawaiian silversword alliance (Compositae-Madiinae). II. Cytogenetics of artificial and natural hybrids

    International Nuclear Information System (INIS)

    Carr, G.D.; Kyhos, D.W.

    1986-01-01

    The Hawaiian silversword alliance of Argyroxiphium, Dubautia, and Wilkesia, in spite of exhibiting spectacular morphological, ecological, physiological, and chromosomal diversity, is remarkably cohesive, genetically. This is attested to by the ease of production of artificial hybrids and by the high frequency of spontaneous hybridization among such life forms as mat-forming subshrub, monocarpic rosette shrub, polycarpic shrub, cushion plant, tree, and vine. Even the least fertile of these hybrids is capable of producing backcross progeny. Moreover, first generation interspecific and intergeneric hybrids have been successfully used to produce trispecific hybrids in a number of instances. In general, the widest hybrid combinations have been as readily produced as crosses within a species. At present eight genomes or chromosome races distinguished by reciprocal translocations are recognized on the basis of meiotic analysis of artificial and spontaneous hybrids. Seven of these races are found among those species with 14 pairs of chromosomes. The eighth genome very likely characterizes all nine species of this alliance that have 13 pairs of chromosomes. The cytogenetic data indicate that redundancy of translocations involving the same chromosomes has been a recurrent theme in the chromosomal differentiation of these taxa. There appears to be little, if any, correlation between chromosomal evolution and adaptive radiation as assessed by gross habital differentiation in this group. However, within Dubautia, a novel ecophysiological trait associated with colonization of xeric habitats is restricted to species with n = 13. In contrast to the bulk of the Hawaiian flora, which is characterized by self-compatibility and chromosomal stability, it is suggested that the occurrence of self-incompatibility in the Hawaiian Madiinae may have favored selection of supergenes via chromosomal repatterning, and this may account for the diversity of chromosome structure seen in this group

  6. The hybrid reactor project based on the straight field line mirror concept

    Science.gov (United States)

    Ågren, O.; Noack, K.; Moiseenko, V. E.; Hagnestâl, A.; Källne, J.; Anglart, H.

    2012-06-01

    The straight field line mirror (SFLM) concept is aiming towards a steady-state compact fusion neutron source. Besides the possibility for steady state operation for a year or more, the geometry is chosen to avoid high loads on materials and plasma facing components. A comparatively small fusion hybrid device with "semi-poor" plasma confinement (with a low fusion Q factor) may be developed for industrial transmutation and energy production from spent nuclear fuel. This opportunity arises from a large fission to fusion energy multiplication ratio, Qr = Pfis/Pfus>>1. The upper bound on Qr is primarily determined by geometry and reactor safety. For the SFLM, the upper bound is Qr≈150, corresponding to a neutron multiplicity of keff=0.97. Power production in a mirror hybrid is predicted for a substantially lower electron temperature than the requirement Te≈10 keV for a fusion reactor. Power production in the SFLM seems possible with Q≈0.15, which is 10 times lower than typically anticipated for hybrids (and 100 times smaller than required for a fusion reactor). This relaxes plasma confinement demands, and broadens the range for use of plasmas with supra-thermal ions in hybrid reactors. The SFLM concept is based on a mirror machine stabilized by qudrupolar magnetic fields and large expander tanks beyond the confinement region. The purpose of the expander tanks is to distribute axial plasma loss flow over a sufficiently large area so that the receiving plates can withstand the heat. Plasma stability is not relying on a plasma flow into the expander regions. With a suppressed plasma flow into the expander tanks, a possibility arise for higher electron temperature. A brief presentation will be given on basic theory for the SFLM with plasma stability and electron temperature issues, RF heating computations with sloshing ion formation, neutron transport computations with reactor safety margins and material load estimates, magnetic coil designs as well as a discussion on

  7. The combined effect of fludarabine monophosphate and radiation on lymphatic cell lines in vitro

    Energy Technology Data Exchange (ETDEWEB)

    Nitsche, M.; Rave-Fraenk, M.; Wolter, C.; Hess, C.F. [Gottingen Univ. Georg-August, Dept. of Radiation Oncology (Germany); Pradier, O. [Centre Hospitalier Universitaire, Dept. de Cancerologie, 29 - Brest (France)

    2006-11-15

    We could demonstrate a fludarabine-induced radiosensitization in two of four cell lines tested. We conclude, that fludarabine may play a role as radiosensitizer. The combined effect of low dose fludarabine and low dose radiation emphasizes the idea of possible multiple reapplication of radio chemotherapy in progressive lymphoma. Further investigations are warranted to identify the potential of this drug as a radiosensitizer in vivo and to elucidate the mechanism of interaction of the drug and radiation. (N.C.)

  8. Modeling of the He-like magnesium spectral lines radiation from the plasma created by XeCl and Nd-glass lasers

    International Nuclear Information System (INIS)

    Stepanov, A. E.; Starostin, A. N.; Roerich, V. C.; Makhrov, V. A.; Faenov, A. Ya.; Magunov, A. I.; Pikuz, T. A.; Skobelev, I. Yu.; Flora, F.; Bollanti, S.; Di Lazzaro, P.; Lisi, N.; Letardi, T.; Palladino, L.; Reale, A.; Batani, D.; Bossi, S.; Bornadinelo, A.; Scafati, A.; Reale, L.

    1997-01-01

    Resonant and intercombination spectral lines formation of He-like magnesium is analyzed both experimentally and numerically. It is shown that in plasma created by XeCl laser at flux density 8·10 12 W/cm 2 the peak of electron temperature is placed downstream from the critical surface at density significantly smaller than critical, and radiation in both resonant and recombination lines is also produced by the plasma region with density below critical. Simulations also show significant line radiation at large distances (1-2 mm) from the target as it was observed in experiments. This secondary peak is produced by a compression wave forming near the plasma front. As opposite, radiation in these lines in plasma created by Nd-glass laser at flux density 5·10 13 W/cm 2 comes from the plasma region placed deeper than the critical surface and has no tail at large distances

  9. Ultralong Radiative States in Hybrid Perovskite Crystals: Compositions for Submillimeter Diffusion Lengths

    KAUST Repository

    Alarousu, Erkki

    2017-08-29

    Organic-inorganic hybrid perovskite materials have recently evolved into the leading candidate solution-processed semiconductor for solar cells due to their combination of desirable optical and charge transport properties. Chief among these properties is the long carrier diffusion length, which is essential to optimizing the device architecture and performance. Herein, we used time-resolved photoluminescence (at low excitation fluence, 10.59 μJ·cm upon two-photon excitation), which is the most accurate and direct approach to measure the radiative charge carrier lifetime and diffusion lengths. Lifetimes of about 72 and 4.3 μs for FAPbBr and FAPbI perovskite single crystals have been recorded, presenting the longest radiative carrier lifetimes reported to date for perovskite materials. Subsequently, carrier diffusion lengths of 107.2 and 19.7 μm are obtained. In addition, we demonstrate the key role of the organic cation units in modulating the carrier lifetime and its diffusion lengths, in which the defect formation energies for FA cations are much higher than those with the MA ones.

  10. Bt-transgenic oilseed rape hybridization with its weedy relative, Brassica rapa.

    Science.gov (United States)

    Halfhill, Matthew D; Millwood, Reginald J; Raymer, Paul L; Stewart, C Neal

    2002-10-01

    The movement of transgenes from crops to weeds and the resulting consequences are concerns of modern agriculture. The possible generation of "superweeds" from the escape of fitness-enhancing transgenes into wild populations is a risk that is often discussed, but rarely studied. Oilseed rape, Brassica napus (L.), is a crop with sexually compatible weedy relatives, such as birdseed rape (Brassica rapa (L.)). Hybridization of this crop with weedy relatives is an extant risk and an excellent interspecific gene flow model system. In laboratory crosses, T3 lines of seven independent transformation events of Bacillus thuringiensis (Bt) oilseed rape were hybridized with two weedy accessions of B. rapa. Transgenic hybrids were generated from six of these oilseed rape lines, and the hybrids exhibited an intermediate morphology between the parental species. The Bt transgene was present in the hybrids, and the protein was synthesized at similar levels to the corresponding independent oilseed rape lines. Insect bioassays were performed and confirmed that the hybrid material was insecticidal. The hybrids were backcrossed with the weedy parent, and only half the oilseed rape lines were able to produce transgenic backcrosses. After two backcrosses, the ploidy level and morphology of the resultant plants were indistinguishable from B. rapa. Hybridization was monitored under field conditions (Tifton, GA, USA) with four independent lines of Bt oilseed rape with a crop to wild relative ratio of 1200:1. When B. rapa was used as the female parent, hybridization frequency varied among oilseed rape lines and ranged from 16.9% to 0.7%.

  11. Hybrid intermediaries

    OpenAIRE

    Cetorelli, Nicola

    2014-01-01

    I introduce the concept of hybrid intermediaries: financial conglomerates that control a multiplicity of entity types active in the "assembly line" process of modern financial intermediation, a system that has become known as shadow banking. The complex bank holding companies of today are the best example of hybrid intermediaries, but I argue that financial firms from the "nonbank" space can just as easily evolve into conglomerates with similar organizational structure, thus acquiring the cap...

  12. Radiation hybrid mapping as one of the main methods of the creation of high resolution maps of human and animal genomes

    International Nuclear Information System (INIS)

    Sulimova, G.E.; Kompanijtsev, A.A.; Mojsyak, E.V.; Rakhmanaliev, Eh.R.; Klimov, E.A.; Udina, I.G.; Zakharov, I.A.

    2000-01-01

    Radiation hybrid mapping (RH mapping) is considered as one of the main method of constructing physical maps of mammalian genomes. In introduction, theoretical prerequisites of developing of the RH mapping and statistical methods of data analysis are discussed. Comparative characteristics of universal commercial panels of the radiation hybrid somatic cells (RH panels) are shown. In experimental part of the work, RH mapping is used to localize nucleotide sequences adjacent to Not I sites of human chromosome 3 with the aim to integrate contig map of Nor I clones to comprehensive maps of human genome. Five nucleotide sequences adjacent to the sites of integration of papilloma virus in human genome and expressed in the cells of cervical cancer involved localized. It is demonstrated that the region 13q14.3-q21.1 was enriched with nucleotide sequences involved in the processes of carcinogenesis. RH mapping can be considered as one of the most perspective applications of modern radiation biology in the field of molecular genetics, that is, in constructing physical maps of mammalian genomes with high resolution level [ru

  13. Cell fusion induced by ionizing radiation in various cell lines

    International Nuclear Information System (INIS)

    Khair, M.B.

    1994-07-01

    Cell fusion induced by ionizing radiation has been studied in rat's hepatocytes in vivo and in different cell lines in vitro. These cell lines were: Hela cells, V-79 fibroblasts, human and rat lymphocytes. For irradiation, 0.85 MeV fission neutrons and 14 MeV fast neutrons were used. Cell analyses were performed by fluorescent dyes using immunofluorescent microscope and flow cytometre. Our results in vivo showed that, regardless the dose-rate, a dose of 1 Gy approximately was enough to induce a significant level of cell fusion depending on neutron energy and the age of rats. The level of cell fusion was also significant in Hela cells at a dose of 0.5 Gy. Similar effect, but to a lesser extent, was observed in V-79 cells. Whereas, in lymphocytes insignificant cell fusion was noticed. The varying levels of cell-fusion in different cell lines could be attributed to the type of cells and mutual contact between cells. Furthermore irradiation did not show any influence on cell division ability in both hepatocytes and Hela cells and that fused cells were also able to divide forming a new generation of cells. (author). 36 refs., 8 figs., 10 tabs

  14. The indirect effect of radiation reduces the repair fidelity of NHEJ as verified in repair deficient CHO cell lines exposed to different radiation qualities and potassium bromate

    International Nuclear Information System (INIS)

    Bajinskis, Ainars; Olsson, Gunilla; Harms-Ringdahl, Mats

    2012-01-01

    The complexity of DNA lesions induced by ionizing radiation is mainly dependent on radiation quality, where the indirect action of radiation may contribute to different extent depending on the type of radiation under study. The effect of indirect action of radiation can be investigated by using agents that induce oxidative DNA damage or by applying free radical scavengers. The aim of this study was to investigate the role of the indirect effect of radiation for the repair fidelity of non-homologous end-joining (NHEJ), homologous recombination repair (HRR) and base excision repair (BER) when DNA damage of different complexity was induced by gamma radiation, alpha particles or from base damages (8-oxo-dG) induced by potassium bromate (KBrO 3 ). CHO cells lines deficient in XRCC3 (HRR) irs1SF, XRCC7 (NHEJ) V3-3 and XRCC1 (BER) EM9 were irradiated in the absence or presence of the free radical scavenger dimethyl sulfoxide (DMSO). The endpoints investigated included rate of cell proliferation by the DRAG assay, clonogenic cell survival and the level of primary DNA damage by the comet assay. The results revealed that the indirect effect of low-LET radiation significantly reduced the repair fidelity of both NHEJ and HRR pathways. For high-LET radiation the indirect effect of radiation also significantly reduced the repair fidelity for the repair deficient cell lines. The results suggest further that the repair fidelity of the error prone NHEJ repair pathway is more impaired by the indirect effect of high-LET radiation relative to the other repair pathways studied. The response to bromate observed for the two DSB repair deficient cell lines strongly support earlier studies that bromate induces complex DNA damages. The significantly reduced repair fidelity of irs1SF and V3-3 suggests that NHEJ as well as HRR are needed for the repair, and that complex DSBs are formed after bromate exposure.

  15. The indirect effect of radiation reduces the repair fidelity of NHEJ as verified in repair deficient CHO cell lines exposed to different radiation qualities and potassium bromate.

    Science.gov (United States)

    Bajinskis, Ainars; Olsson, Gunilla; Harms-Ringdahl, Mats

    2012-03-01

    The complexity of DNA lesions induced by ionizing radiation is mainly dependent on radiation quality, where the indirect action of radiation may contribute to different extent depending on the type of radiation under study. The effect of indirect action of radiation can be investigated by using agents that induce oxidative DNA damage or by applying free radical scavengers. The aim of this study was to investigate the role of the indirect effect of radiation for the repair fidelity of non-homologous end-joining (NHEJ), homologous recombination repair (HRR) and base excision repair (BER) when DNA damage of different complexity was induced by gamma radiation, alpha particles or from base damages (8-oxo-dG) induced by potassium bromate (KBrO(3)). CHO cells lines deficient in XRCC3 (HRR) irs1SF, XRCC7 (NHEJ) V3-3 and XRCC1 (BER) EM9 were irradiated in the absence or presence of the free radical scavenger dimethyl sulfoxide (DMSO). The endpoints investigated included rate of cell proliferation by the DRAG assay, clonogenic cell survival and the level of primary DNA damage by the comet assay. The results revealed that the indirect effect of low-LET radiation significantly reduced the repair fidelity of both NHEJ and HRR pathways. For high-LET radiation the indirect effect of radiation also significantly reduced the repair fidelity for the repair deficient cell lines. The results suggest further that the repair fidelity of the error prone NHEJ repair pathway is more impaired by the indirect effect of high-LET radiation relative to the other repair pathways studied. The response to bromate observed for the two DSB repair deficient cell lines strongly support earlier studies that bromate induces complex DNA damages. The significantly reduced repair fidelity of irs1SF and V3-3 suggests that NHEJ as well as HRR are needed for the repair, and that complex DSBs are formed after bromate exposure. Copyright © 2011 Elsevier B.V. All rights reserved.

  16. The indirect effect of radiation reduces the repair fidelity of NHEJ as verified in repair deficient CHO cell lines exposed to different radiation qualities and potassium bromate

    Energy Technology Data Exchange (ETDEWEB)

    Bajinskis, Ainars, E-mail: ainars.bajinskis@gmt.su.se [Centre for Radiation Protection Research, Department of Genetics, Microbiology and Toxicology, Stockholm University, S-10691 Stockholm (Sweden); Olsson, Gunilla; Harms-Ringdahl, Mats [Centre for Radiation Protection Research, Department of Genetics, Microbiology and Toxicology, Stockholm University, S-10691 Stockholm (Sweden)

    2012-03-01

    The complexity of DNA lesions induced by ionizing radiation is mainly dependent on radiation quality, where the indirect action of radiation may contribute to different extent depending on the type of radiation under study. The effect of indirect action of radiation can be investigated by using agents that induce oxidative DNA damage or by applying free radical scavengers. The aim of this study was to investigate the role of the indirect effect of radiation for the repair fidelity of non-homologous end-joining (NHEJ), homologous recombination repair (HRR) and base excision repair (BER) when DNA damage of different complexity was induced by gamma radiation, alpha particles or from base damages (8-oxo-dG) induced by potassium bromate (KBrO{sub 3}). CHO cells lines deficient in XRCC3 (HRR) irs1SF, XRCC7 (NHEJ) V3-3 and XRCC1 (BER) EM9 were irradiated in the absence or presence of the free radical scavenger dimethyl sulfoxide (DMSO). The endpoints investigated included rate of cell proliferation by the DRAG assay, clonogenic cell survival and the level of primary DNA damage by the comet assay. The results revealed that the indirect effect of low-LET radiation significantly reduced the repair fidelity of both NHEJ and HRR pathways. For high-LET radiation the indirect effect of radiation also significantly reduced the repair fidelity for the repair deficient cell lines. The results suggest further that the repair fidelity of the error prone NHEJ repair pathway is more impaired by the indirect effect of high-LET radiation relative to the other repair pathways studied. The response to bromate observed for the two DSB repair deficient cell lines strongly support earlier studies that bromate induces complex DNA damages. The significantly reduced repair fidelity of irs1SF and V3-3 suggests that NHEJ as well as HRR are needed for the repair, and that complex DSBs are formed after bromate exposure.

  17. Development of a 144-channel Hybrid Avalanche Photo-Detector for Belle II ring-imaging Cherenkov counter with an aerogel radiator

    Energy Technology Data Exchange (ETDEWEB)

    Nishida, S., E-mail: shohei.nishida@kek.jp [High Energy Accelerator Research Organization (KEK), Tsukuba (Japan); Adachi, I. [High Energy Accelerator Research Organization (KEK), Tsukuba (Japan); Hamada, N. [Toho University, Funabashi (Japan); Hara, K. [High Energy Accelerator Research Organization (KEK), Tsukuba (Japan); Iijima, T. [Nagoya University, Nagoya (Japan); Iwata, S.; Kakuno, H. [Tokyo Metropolitan University, Hachioji (Japan); Kawai, H. [Chiba University, Chiba (Japan); Korpar, S.; Krizan, P. [Jozef Stefan Institute, Ljubljana (Slovenia); Ogawa, S. [Toho University, Funabashi (Japan); Pestotnik, R.; Ŝantelj, L.; Seljak, A. [Jozef Stefan Institute, Ljubljana (Slovenia); Sumiyoshi, T. [Tokyo Metropolitan University, Hachioji (Japan); Tabata, M. [Chiba University, Chiba (Japan); Tahirovic, E. [Jozef Stefan Institute, Ljubljana (Slovenia); Yoshida, K. [Tokyo Metropolitan University, Hachioji (Japan); Yusa, Y. [Niigata University, Niigata (Japan)

    2015-07-01

    The Belle II detector, a follow up of the very successful Belle experiment, is under construction at the SuperKEKB electron–positron collider at KEK in Japan. For the PID system in the forward region of the spectrometer, a proximity-focusing ring-imaging Cherenkov counter with an aerogel radiator is being developed. For the position sensitive photon sensor, a 144-channel Hybrid Avalanche Photo-Detector has been developed with Hamamatsu Photonics K.K. In this report, we describe the specification of the Hybrid Avalanche Photo-Detector and the status of the mass production.

  18. Scattering of lower-hybrid waves by drift-wave density fluctuations: solutions of the radiative transfer equation

    International Nuclear Information System (INIS)

    Andrews, P.L.; Perkins, F.W.

    1983-01-01

    The investigation of the scattering of lower-hybrid waves by density fluctuations arising from drift waves in tokamaks is distinguished by the presence in the wave equation of a large, random, derivative-coupling term. The propagation of the lower-hybrid waves is well represented by a radiative transfer equation when the scale size of the density fluctuations is small compared to the overall plasma size. The radiative transfer equation is solved in two limits: first, the forward scattering limit, where the scale size of density fluctuations is large compared to the lower-hybrid perpendicular wavelength, and second, the large-angle scattering limit, where this inequality is reversed. The most important features of these solutions are well represented by analytical formulas derived by simple arguments. Based on conventional estimates for density fluctuations arising from drift waves and a parabolic density profile, the optical depth tau for scattering through a significant angle, is given by tauroughly-equal(2/N 2 /sub parallel/) (#betta#/sub p/i0/#betta#) 2 (m/sub e/c 2 /2T/sub i/)/sup 1/2/ [c/α(Ω/sub i/Ω/sub e/)/sup 1/2/ ], where #betta#/sub p/i0 is the central ion plasma frequency and T/sub i/ denotes the ion temperature near the edge of the plasma. Most of the scattering occurs near the surface. The transmission through the scattering region scales as tau - 1 and the emerging intensity has an angular spectrum proportional to cos theta, where sin theta = k/sub perpendicular/xB/sub p//(k/sub perpendicular/B/sub p/), and B/sub p/ is the poloidal field

  19. Monte Carlo simulation of simultaneous radiation detection in the hybrid tomography system ClearPET-XPAD3/CT

    Energy Technology Data Exchange (ETDEWEB)

    Dávila, H. Olaya, E-mail: hernan.olaya@uptc.edu.co; Martínez, S. A. [Physics Department, Universidad Pedagógica y Tecnológica de Colombia, Tunja-Colombia (Colombia); Sevilla, A. C., E-mail: acsevillam@unal.edu.co; Castro, H. F. [Physics Department, Universidad Nacional de Colombia, Bogotá D.C - Colombia (Colombia)

    2016-07-07

    Using the Geant4 based simulation framework SciFW1, a detailed simulation was performed for a detector array in the hybrid tomography prototype for small animals called ClearPET / XPAD, which was built in the Centre de Physique des Particules de Marseille. The detector system consists of an array of phoswich scintillation detectors: LSO (Lutetium Oxy-ortosilicate doped with cerium Lu{sub 2}SiO{sub 5}:Ce) and LuYAP (Lutetium Ortoaluminate of Yttrium doped with cerium Lu{sub 0.7}Y{sub 0.3}AlO{sub 3}:Ce) for Positron Emission Tomography (PET) and hybrid pixel detector XPAD for Computed Tomography (CT). Simultaneous acquisition of deposited energy and the corresponding time - position for each recorded event were analyzed, independently, for both detectors. interference between detection modules for PET and CT. Information about amount of radiation reaching each phoswich crystal and XPAD detector using a phantom in order to study the effectiveness by radiation attenuation and influence the positioning of the radioactive source {sup 22}Na was obtained. The simulation proposed will improve distribution of detectors rings and interference values will be taken into account in the new versions of detectors.

  20. Monte Carlo simulation of simultaneous radiation detection in the hybrid tomography system ClearPET-XPAD3/CT

    Science.gov (United States)

    Dávila, H. Olaya; Sevilla, A. C.; Castro, H. F.; Martínez, S. A.

    2016-07-01

    Using the Geant4 based simulation framework SciFW1, a detailed simulation was performed for a detector array in the hybrid tomography prototype for small animals called ClearPET / XPAD, which was built in the Centre de Physique des Particules de Marseille. The detector system consists of an array of phoswich scintillation detectors: LSO (Lutetium Oxy-ortosilicate doped with cerium Lu2SiO5:Ce) and LuYAP (Lutetium Ortoaluminate of Yttrium doped with cerium Lu0.7Y0.3AlO3:Ce) for Positron Emission Tomography (PET) and hybrid pixel detector XPAD for Computed Tomography (CT). Simultaneous acquisition of deposited energy and the corresponding time - position for each recorded event were analyzed, independently, for both detectors. interference between detection modules for PET and CT. Information about amount of radiation reaching each phoswich crystal and XPAD detector using a phantom in order to study the effectiveness by radiation attenuation and influence the positioning of the radioactive source 22Na was obtained. The simulation proposed will improve distribution of detectors rings and interference values will be taken into account in the new versions of detectors.

  1. System for cooling hybrid vehicle electronics, method for cooling hybrid vehicle electronics

    Science.gov (United States)

    France, David M.; Yu, Wenhua; Singh, Dileep; Zhao, Weihuan

    2017-11-21

    The invention provides a single radiator cooling system for use in hybrid electric vehicles, the system comprising a surface in thermal communication with electronics, and subcooled boiling fluid contacting the surface. The invention also provides a single radiator method for simultaneously cooling electronics and an internal combustion engine in a hybrid electric vehicle, the method comprising separating a coolant fluid into a first portion and a second portion; directing the first portion to the electronics and the second portion to the internal combustion engine for a time sufficient to maintain the temperature of the electronics at or below 175.degree. C.; combining the first and second portion to reestablish the coolant fluid; and treating the reestablished coolant fluid to the single radiator for a time sufficient to decrease the temperature of the reestablished coolant fluid to the temperature it had before separation.

  2. Effects of external radiation fields on line emission—application to star-forming regions

    Energy Technology Data Exchange (ETDEWEB)

    Chatzikos, Marios; Ferland, G. J. [University of Kentucky, Lexington, KY 40506 (United States); Williams, R. J. R. [AWE plc, Aldermaston, Reading RG7 4PR (United Kingdom); Porter, Ryan [Department of Physics and Astronomy and Center for Simulational Physics, University of Georgia, Athens, GA 30602-2451 (United States); Van Hoof, P. A. M., E-mail: mchatzikos@gmail.com [Royal Observatory of Belgium, Avenue Circulaire 3, B-1180 Uccle (Belgium)

    2013-12-20

    A variety of astronomical environments contain clouds irradiated by a combination of isotropic and beamed radiation fields. For example, molecular clouds may be irradiated by the isotropic cosmic microwave background, as well as by a nearby active galactic nucleus. These radiation fields excite atoms and molecules and produce emission in different ways. We revisit the escape probability theorem and derive a novel expression that accounts for the presence of external radiation fields. We show that when the field is isotropic the escape probability is reduced relative to that in the absence of external radiation. This is in agreement with previous results obtained under ad hoc assumptions or with the two-level system, but can be applied to complex many-level models of atoms or molecules. This treatment is in the development version of the spectral synthesis code CLOUDY. We examine the spectrum of a Spitzer cloud embedded in the local interstellar radiation field and show that about 60% of its emission lines are sensitive to background subtraction. We argue that this geometric approach could provide an additional tool toward understanding the complex radiation fields of starburst galaxies.

  3. Radiation protective effect of hypoxia-inducible factor-1α (HIF-1α) on human oral squamous cell carcinoma cell lines

    International Nuclear Information System (INIS)

    Hosokawa, Y.; Okumura, K.; Terashima, S.; Sakakura, Y.

    2012-01-01

    We examined the effects of 5-Gy radiation on the expression of hypoxia-inducible factor-1α (HIF-1α) and the radiosensitivity of five human oral squamous cell carcinoma (OSCC) cell lines (SAS, Ca9-22, TT, BSC-OF and IS-FOM). In all of the cell lines, HIF-1α was expressed in mRNA, and radiation had no influence on gene transcription. The number of apoptotic cells increased 72 h after irradiation in cell lines SAS, Ca9-22 and TT cells, indicating low transcriptional levels of HIF-1α, and the levels of non-cleaved caspase-3, an executioner of apoptosis, and non-cleaved poly (adenosine diphosphate-ribose) polymerase (PARP), a marker of DNA damage early in apoptosis, decreased simultaneously. Conversely, radiation failed to induce apoptosis or to decrease expression of non-cleaved caspase-3 and PARP in cell-lines BSC-OF and IS-FOM cells that expressed high levels of HIF-1α. BSC-OF and IS-FOM cells exhibited high migratory capacity. When CoCl 2 was present in the medium, HIF-1α expression increased along with the survival of Ca9-22 cells after radiation exposure. These results suggest that OSCC cells expressing high levels of HIF-1α are resistant to radiation. HIF-1α can be used to control the short term radiosensitivity of cells. (authors)

  4. An Entropy-Based Adaptive Hybrid Particle Swarm Optimization for Disassembly Line Balancing Problems

    Directory of Open Access Journals (Sweden)

    Shanli Xiao

    2017-11-01

    Full Text Available In order to improve the product disassembly efficiency, the disassembly line balancing problem (DLBP is transformed into a problem of searching for the optimum path in the directed and weighted graph by constructing the disassembly hierarchy information graph (DHIG. Then, combining the characteristic of the disassembly sequence, an entropy-based adaptive hybrid particle swarm optimization algorithm (AHPSO is presented. In this algorithm, entropy is introduced to measure the changing tendency of population diversity, and the dimension learning, crossover and mutation operator are used to increase the probability of producing feasible disassembly solutions (FDS. Performance of the proposed methodology is tested on the primary problem instances available in the literature, and the results are compared with other evolutionary algorithms. The results show that the proposed algorithm is efficient to solve the complex DLBP.

  5. Intrinsic line shape of electromagnetic radiation from a stack of intrinsic Josephson junctions synchronized by an internal cavity resonance

    Science.gov (United States)

    Koshelev, Alexei

    2013-03-01

    Stacks of intrinsic Josephson-junctions are realized in mesas fabricated out of layered superconducting single crystals, such as Bi2Sr2CaCu2O8 (BSCCO). Synchronization of phase oscillations in different junctions can be facilitated by the coupling to the internal cavity mode leading to powerful and coherent electromagnetic radiation in the terahertz frequency range. An important characteristic of this radiation is the shape of the emission line. A finite line width appears due to different noise sources leading to phase diffusion. We investigated the intrinsic line shape caused by the thermal noise for a mesa fabricated on the top of a BSCCO single crystal. In the ideal case of fully synchronized stack the finite line width is coming from two main contributions, the quasiparticle-current noise inside the mesa and the fluctuating radiation in the base crystal. We compute both contributions and conclude that for realistic mesa's parameters the second mechanism typically dominates. The role of the cavity quality factor in the emission line spectrum is clarified. Analytical results were verified by numerical simulations. In real mesa structures part of the stack may not be synchronized and chaotic dynamics of unsynchronized junctions may determine the real line width. Work supported by UChicago Argonne, LLC, under contract No. DE-AC02-06CH11357.

  6. A functional genomics approach using radiation-induced changes in gene expression to study low dose radiation effects in vitro and in vivo

    Energy Technology Data Exchange (ETDEWEB)

    Fornace, Jr, A J

    2007-03-03

    Abstract for final report for project entitled A functional genomics approach using radiation-induced changes in gene expression to study low dose radiation effects in vitro and in vivo which has been supported by the DOE Low Dose Radiation Research Program for approximately 7 years. This project has encompassed two sequential awards, ER62683 and then ER63308, in the Gene Response Section in the Center for Cancer Research at the National Cancer Institute. The project was temporarily suspended during the relocation of the Principal Investigators laboratory to the Dept. of Genetics and Complex Diseases at Harvard School of Public Health at the end of 2004. Remaining support for the final year was transferred to this new site later in 2005 and was assigned the DOE Award Number ER64065. The major aims of this project have been 1) to characterize changes in gene expression in response to low-dose radiation responses; this includes responses in human cells lines, peripheral blood lymphocytes (PBL), and in vivo after human or murine exposures, as well as the effect of dose-rate on gene responses; 2) to characterize changes in gene expression that may be involved in bystander effects, such as may be mediated by cytokines and other intercellular signaling proteins; and 3) to characterize responses in transgenic mouse models with relevance to genomic stability. A variety of approaches have been used to study transcriptional events including microarray hybridization, quantitative single-probe hybridization which was developed in this laboratory, quantitative RT-PCR, and promoter microarray analysis using genomic regulatory motifs. Considering the frequent responsiveness of genes encoding cytokines and related signaling proteins that can affect cellular metabolism, initial efforts were initiated to study radiation responses at the metabolomic level and to correlate with radiation-responsive gene expression. Productivity includes twenty-four published and in press manuscripts

  7. Computational lymphatic node models in pediatric and adult hybrid phantoms for radiation dosimetry

    International Nuclear Information System (INIS)

    Lee, Choonsik; Lamart, Stephanie; Moroz, Brian E

    2013-01-01

    We developed models of lymphatic nodes for six pediatric and two adult hybrid computational phantoms to calculate the lymphatic node dose estimates from external and internal radiation exposures. We derived the number of lymphatic nodes from the recommendations in International Commission on Radiological Protection (ICRP) Publications 23 and 89 at 16 cluster locations for the lymphatic nodes: extrathoracic, cervical, thoracic (upper and lower), breast (left and right), mesentery (left and right), axillary (left and right), cubital (left and right), inguinal (left and right) and popliteal (left and right), for different ages (newborn, 1-, 5-, 10-, 15-year-old and adult). We modeled each lymphatic node within the voxel format of the hybrid phantoms by assuming that all nodes have identical size derived from published data except narrow cluster sites. The lymph nodes were generated by the following algorithm: (1) selection of the lymph node site among the 16 cluster sites; (2) random sampling of the location of the lymph node within a spherical space centered at the chosen cluster site; (3) creation of the sphere or ovoid of tissue representing the node based on lymphatic node characteristics defined in ICRP Publications 23 and 89. We created lymph nodes until the pre-defined number of lymphatic nodes at the selected cluster site was reached. This algorithm was applied to pediatric (newborn, 1-, 5-and 10-year-old male, and 15-year-old males) and adult male and female ICRP-compliant hybrid phantoms after voxelization. To assess the performance of our models for internal dosimetry, we calculated dose conversion coefficients, called S values, for selected organs and tissues with Iodine-131 distributed in six lymphatic node cluster sites using MCNPX2.6, a well validated Monte Carlo radiation transport code. Our analysis of the calculations indicates that the S values were significantly affected by the location of the lymph node clusters and that the values increased for

  8. On-Line Radiation Test Facility for Industrial Equipment needed for the Large Hadron Collider at CERN

    CERN Document Server

    Rausch, R

    1999-01-01

    The future Large Hadron Collider to be built at CERN will use superconducting magnets cooled down to 1.2 K. To preserve the superconductivity, the energy deposition dose levels in equipment located outside the cryostat, in the LHC tunnel, are calculated to be of the order of 1 to 10 Gy per year. At such dose levels, no major radiation-damage problems are to be expected, and the possibility of installing Commercial Of The Shelf (COTS) electronic equipment in the LHC tunnel along the accelerator is considered. To this purpose, industrial electronic equipment and circuits have to be qualified and tested against radiation to insure their long term stability and reliability. An on-line radiation test facility has been setup at the CERN Super Proton Synchrotron (SPS) and a program of on-line tests for electronic equipment is ongoing. Equipment tested includes Industrial Programmable Logic Controllers (PLCs) from several manufacturers, standard VME modules, Fieldbuses like Profibus, WorldFIP and CAN, various electro...

  9. Detailed properties of magnetospheric line radiation events observed by the DEMETER spacecraft

    Czech Academy of Sciences Publication Activity Database

    Němec, F.; Parrot, M.; Santolík, Ondřej

    2012-01-01

    Roč. 117, A5 (2012), A05210/1-A05210/9 ISSN 0148-0227 R&D Projects: GA ČR GA205/09/1253; GA MŠk ME09107 Institutional support: RVO:68378289 Keywords : magnetospheric line radiation * electromagnetic waves Subject RIV: BL - Plasma and Gas Discharge Physics Impact factor: 3.174, year: 2012 http://onlinelibrary.wiley.com/doi/10.1029/2012JA017517/abstract

  10. Measurements of national radiation exposure rates on train lines in Tokai area

    International Nuclear Information System (INIS)

    Matsuda, Hideharu

    1996-01-01

    For data accumulation of natural radiation exposure rate derived from gamma-ray and cosmic-ray to evaluate population dose, the author measured the rate in the running vehicles of 12 JR Tokai lines, 17 Nagoya Railway lines, 4 Kinkinippon Railway lines and 1 line of Nagoya City Bus. A portable gamma spectrometer equipped with 3' in diameter x 3' NaI (Tl) scintillation detector was placed on the seat of the vehicle for measurement in the period of December, 1992-August, 1995. Gamma-ray and cosmic-ray exposure rates in air were assessed separately as reported before and expressed in Gy/h. The average exposure rate of gamma-ray in JR Tokai lines was 19.8 nGy/h and of cosmic-ray, 28.5 nGy/h, both of which were markedly varied from line to line. The average rates of gamma-and cosmic-ray were 21.6 nGy/h and 29.0 nGy/h, respectively, in Nagoya Railway lines and 20.9 nGy/h and 28.7 nGy/h, respectively, in Kinkinippon lines. In the city bus, the respective rates were 27.2 nGy/h and 27.0 nGy/h. Thus, the average rates of gamma-ray (about 20 nGy/h) and cosmic-ray (about 29 nGy/h) were not so different between JR and other private railway lines. In the bus, the former rate was slightly lower and the latter, slightly higher. However, the total rates of both rays were in the range of about 50-55 nGy/h in all vehicles examined. (H.O.)

  11. THE STAGES OF HETEROTIC HYBRIDS F1 DEVELOPMENT IN EUROPEAN RADISH

    Directory of Open Access Journals (Sweden)

    M. A. Kosenko

    2017-01-01

    Full Text Available The scheme  of  development  of  two-line  of  hybrids  F1  in European radish based on self-incompatibility includes five stages, as follows: 1 – selection of self-incompatible lines, common and specified combining ability estimation; 2 – inbreeding and selection to make the lines homozygous for morphological traits, common and specified combining ability estimation; maintenance and reproduction of self-incompatible lines; 4 – production of hybrid seeds. The research work on assessment of hybrid F1 that were obtained from cross of eight self-incompatible lines of European winter radish by the Griffing’s method was carried out in 2016. The assessment of length, diameter and yield of radish root was performed. According to the root shape the heterotic hybrids F1 were divided into three groups: rounded-flat,  48.2%; round, 50.0%; and flatten-round,  1.8%. The level of root marketability of hybrids F1 reached 100%. As a result of the work the promising hybrid combination distinguished by high uniformity, marketability and high yield were selected out.

  12. Induction of chromosome aberrations in two lines of cultured cells using different types of radiation

    International Nuclear Information System (INIS)

    Zoetelief, J.; Dingjan-Hirschi, E.S.; Hasper, J.; Janse, H.C.; Barendsen, G.W.

    The induction of chromosome aberrations has been investigated in two lines of cultured cells for different types of radiation. The obtained results are compared with information on induction of cell reproductive death and malignant transformation. (Auth.)

  13. Performance evaluation of stand alone hybrid PV-wind generator

    Energy Technology Data Exchange (ETDEWEB)

    Nasir, M. N. M.; Saharuddin, N. Z.; Sulaima, M. F.; Jali, Mohd Hafiz; Bukhari, W. M.; Bohari, Z. H. [Faculty of Electrical Engineering, Universiti Teknikal Malaysia Melaka (UTeM), Hang Tuah Jaya, 76100 Melaka (Malaysia); Yahaya, M. S. [Faculty of Engineering Technology, Universiti Teknikal Malaysia Melaka (UTeM), Hang Tuah Jaya, 76100 Melaka (Malaysia)

    2015-05-15

    This paper presents the performance evaluation of standalone hybrid system on Photovoltaic (PV)-Wind generator at Faculty of Electrical Engineering (FKE), UTeM. The hybrid PV-Wind in UTeM system is combining wind turbine system with the solar system and the energy capacity of this hybrid system can generate up to charge the battery and supply the LED street lighting load. The purpose of this project is to evaluate the performance of PV-Wind hybrid generator. Solar radiation meter has been used to measure the solar radiation and anemometer has been used to measure the wind speed. The effectiveness of the PV-Wind system is based on the various data that has been collected and compared between them. The result shows that hybrid system has greater reliability. Based on the solar result, the correlation coefficient shows strong relationship between the two variables of radiation and current. The reading output current followed by fluctuate of solar radiation. However, the correlation coefficient is shows moderate relationship between the two variables of wind speed and voltage. Hence, the wind turbine system in FKE show does not operate consistently to produce energy source for this hybrid system compare to PV system. When the wind system does not fully operate due to inconsistent energy source, the other system which is PV will operate and supply the load for equilibrate the extra load demand.

  14. Performance evaluation of stand alone hybrid PV-wind generator

    Science.gov (United States)

    Nasir, M. N. M.; Saharuddin, N. Z.; Sulaima, M. F.; Jali, Mohd Hafiz; Bukhari, W. M.; Bohari, Z. H.; Yahaya, M. S.

    2015-05-01

    This paper presents the performance evaluation of standalone hybrid system on Photovoltaic (PV)-Wind generator at Faculty of Electrical Engineering (FKE), UTeM. The hybrid PV-Wind in UTeM system is combining wind turbine system with the solar system and the energy capacity of this hybrid system can generate up to charge the battery and supply the LED street lighting load. The purpose of this project is to evaluate the performance of PV-Wind hybrid generator. Solar radiation meter has been used to measure the solar radiation and anemometer has been used to measure the wind speed. The effectiveness of the PV-Wind system is based on the various data that has been collected and compared between them. The result shows that hybrid system has greater reliability. Based on the solar result, the correlation coefficient shows strong relationship between the two variables of radiation and current. The reading output current followed by fluctuate of solar radiation. However, the correlation coefficient is shows moderate relationship between the two variables of wind speed and voltage. Hence, the wind turbine system in FKE show does not operate consistently to produce energy source for this hybrid system compare to PV system. When the wind system does not fully operate due to inconsistent energy source, the other system which is PV will operate and supply the load for equilibrate the extra load demand.

  15. Performance evaluation of stand alone hybrid PV-wind generator

    International Nuclear Information System (INIS)

    Nasir, M. N. M.; Saharuddin, N. Z.; Sulaima, M. F.; Jali, Mohd Hafiz; Bukhari, W. M.; Bohari, Z. H.; Yahaya, M. S.

    2015-01-01

    This paper presents the performance evaluation of standalone hybrid system on Photovoltaic (PV)-Wind generator at Faculty of Electrical Engineering (FKE), UTeM. The hybrid PV-Wind in UTeM system is combining wind turbine system with the solar system and the energy capacity of this hybrid system can generate up to charge the battery and supply the LED street lighting load. The purpose of this project is to evaluate the performance of PV-Wind hybrid generator. Solar radiation meter has been used to measure the solar radiation and anemometer has been used to measure the wind speed. The effectiveness of the PV-Wind system is based on the various data that has been collected and compared between them. The result shows that hybrid system has greater reliability. Based on the solar result, the correlation coefficient shows strong relationship between the two variables of radiation and current. The reading output current followed by fluctuate of solar radiation. However, the correlation coefficient is shows moderate relationship between the two variables of wind speed and voltage. Hence, the wind turbine system in FKE show does not operate consistently to produce energy source for this hybrid system compare to PV system. When the wind system does not fully operate due to inconsistent energy source, the other system which is PV will operate and supply the load for equilibrate the extra load demand

  16. Radiation preparation of graphene/carbon nanotubes hybrid fillers for mechanical reinforcement of poly(vinyl alcohol) films

    Science.gov (United States)

    Ma, Hui-Ling; Zhang, Long; Zhang, Youwei; Wang, Shuojue; Sun, Chao; Yu, Hongyan; Zeng, Xinmiao; Zhai, Maolin

    2016-01-01

    Graphene/carbon nanotubes (G/CNTs) hybrid fillers were synthesized by γ-ray radiation reduction of graphene oxide (GO) in presence of CNTs. The obtained hybrid fillers with three-dimensional (3D) interconnected network structure were characterized by X-ray diffraction (XRD) and scanning electron microscope (SEM). Poly(vinyl alcohol) (PVA) composite films with enhanced mechanical properties and thermal stability were subsequently prepared by solution blending of G/CNTs with PVA matrix. The tensile strength and Young's modulus of PVA composite films containing 1 wt% G/CNTs were measured to be 81.9 MPa and 3.9 GPa respectively, which were 56% and 33.6% higher than those of pure PVA. These substantial improvements could be attributed to the interconnected 3D structure of G/CNTs, homogeneous dispersion as well as the strong hydrogen-bonding interaction between G/CNTs and PVA macromolecular chains.

  17. The hybrid reactor project based on the straight field line mirror concept

    International Nuclear Information System (INIS)

    Ågren, O.; Noack, K.; Moiseenko, V. E.; Hagnestål, A.; Källne, J.; Anglart, H.

    2012-01-01

    The straight field line mirror (SFLM) concept is aiming towards a steady-state compact fusion neutron source. Besides the possibility for steady state operation for a year or more, the geometry is chosen to avoid high loads on materials and plasma facing components. A comparatively small fusion hybrid device with “semi-poor” plasma confinement (with a low fusion Q factor) may be developed for industrial transmutation and energy production from spent nuclear fuel. This opportunity arises from a large fission to fusion energy multiplication ratio, Q r = P fis /P fus >>1. The upper bound on Q r is primarily determined by geometry and reactor safety. For the SFLM, the upper bound is Q r ≈150, corresponding to a neutron multiplicity of k eff =0.97. Power production in a mirror hybrid is predicted for a substantially lower electron temperature than the requirement T e ≈10 keV for a fusion reactor. Power production in the SFLM seems possible with Q≈0.15, which is 10 times lower than typically anticipated for hybrids (and 100 times smaller than required for a fusion reactor). This relaxes plasma confinement demands, and broadens the range for use of plasmas with supra-thermal ions in hybrid reactors. The SFLM concept is based on a mirror machine stabilized by qudrupolar magnetic fields and large expander tanks beyond the confinement region. The purpose of the expander tanks is to distribute axial plasma loss flow over a sufficiently large area so that the receiving plates can withstand the heat. Plasma stability is not relying on a plasma flow into the expander regions. With a suppressed plasma flow into the expander tanks, a possibility arise for higher electron temperature. A brief presentation will be given on basic theory for the SFLM with plasma stability and electron temperature issues, RF heating computations with sloshing ion formation, neutron transport computations with reactor safety margins and material load estimates, magnetic coil designs as well as

  18. Borneo: The new NS sunflower confectionary type hybrid

    Directory of Open Access Journals (Sweden)

    Hladni Nada

    2009-01-01

    Full Text Available At the Institute of Field and Vegetable Crops in Novi Sad, beside the basic direction in sunflower breeding, the creation of standard hybrids with high oil content with high seed and oil yield, hybrids are being created for special confectionery purposes directed towards the final kernel products. In this program, apart from breeding in order to improve main seed yield components, special attention is given to the increase of protein content and the quality with the decrease of seed oil content, to the increase of the weight of 1000 seeds and to the decrease of content of the shell. Two-line SC hybrid was created by crossing the cytoplasmatic male sterile line of the mother with a father line with fertility restoration genes. Borneo is a high protein confectionary type hybrid that has been registered in Slovakia (EU in 2009. It contains successfully combined genes responsible for high genetic potential for yield and valuable technical and technological seed traits. The hybrid is adequate for nutrition, dehuling and kernel production. Borneo is a medium early hybrid with the genetic potential for yield of over 4,5t/ha and its seed oil content is under 43%. .

  19. Relationship between the intensity of nonthermal radiation and widths of Balmer lines in the spectra of quasar-like nuclei

    International Nuclear Information System (INIS)

    Komberg, B.V.; Shefer, E.Yu.

    1983-01-01

    The data summarized by Steiner (1981) were used to derive the relationship between the intensity of nonthermal X-ray and radio emission and fullwidths of Balmer emission lines. It is shown that the existence of such dependences does not contradict the assumption on partition equidistribution of nonthermal radiation and motion of gas clouds energy in the region of formation of wide lines. Emphasis is given to similar dependence drived by Vayana (1981) for X-ray radiation from hot coronas of fast-rotating single stars, and a suggestion is made about radiation anisotropy from active nuclei (similar to that observed in the SS 433 system). The latter may imply the double nature of active nuclei

  20. The evaluation of radiation level and dose of workers in Guangzhou metro line 1

    International Nuclear Information System (INIS)

    Zhang Lin; Hu Canyun; Meng Xiaolian; He Zhan

    2006-01-01

    Objective: To find out the level of radiation and effective dose of workers in Guangzhou Metro line 1. Methods: In metro stations, external Gamma-ray exposure rates were obtained by FD-71A radiance measurer, 222 Rn and 220 Rn concentrations were obtained by using Rn-Tn solid state nuclear track detectors developed by The National Institute for Radiological Protection and Nuclear Safety, Chinese Centre for Disease Control. The annual Effective dose from Gamma, 222 Rn and 220 Rn were calculated. Results: The external Gamma-ray exposure average rate is 17.74 x 10 -8 Gy/h. The average concentration of 222 Rn is 59.8 Bq/m 3 . The average concentration of 220 Rn is 32.1 Bq/m 3 . The total annual effective dose from Gamma, 222 Rn and 220 Rn is 2.878 mSv/a. Conclusion: In the stations of Guangzhou in metro line 1, no more effective radiation dose to the workers has measured. (authors)

  1. The effect on radiation damage of structural material in a hybrid system by using a Monte Carlo radiation transport code

    International Nuclear Information System (INIS)

    Günay, Mehtap; Şarer, Başar; Kasap, Hızır

    2014-01-01

    Highlights: • The effects of some fluids on gas production rates in structural material were investigated. • The MCNPX-2.7.0 Monte Carlo code was used for three-dimensional calculations. • It was found that biggest contribution to gas production rates comes from Fe isotope of the. • The desirable values for 5% SFG-PuO 2 with respect to radiation damage were specified. - Abstract: In this study, the molten salt-heavy metal mixtures 99–95% Li20Sn80-1-5% SFG-Pu, 99–95% Li20Sn80-1-5% SFG-PuF4, 99-95% Li20Sn80-1-5% SFG-PuO2 were used as fluids. The fluids were used in the liquid first-wall, blanket and shield zones of the designed hybrid reactor system. 9Cr2WVTa ferritic steel with the width of 4 cm was used as the structural material. The parameters of radiation damage are proton, deuterium, tritium, He-3 and He-4 gas production rates. In this study, the effects of the selected fluid on the radiation damage, in terms of individual as well as total isotopes in the structural material, were investigated for 30 full power years (FPYs). Three-dimensional analyses were performed using the most recent version of the MCNPX-2.7.0 Monte Carlo radiation transport code and the ENDF/B-VII.0 nuclear data library

  2. Excitation of hybridized Dirac plasmon polaritons and transition radiation in multi-layer graphene traversed by a fast charged particle

    Science.gov (United States)

    Akbari, Kamran; Mišković, Zoran L.; Segui, Silvina; Gervasoni, Juana L.; Arista, Néstor R.

    2018-06-01

    We analyze the energy loss channels for a fast charged particle traversing a multi-layer graphene (MLG) structure with N layers under normal incidence. Focusing on a terahertz (THz) range of frequencies, and assuming equally doped graphene layers with a large enough separation d between them to neglect interlayer electron hopping, we use the Drude model for two-dimensional conductivity of each layer to describe hybridization of graphene’s Dirac plasmon polaritons (DPPs). Performing a layer decomposition of ohmic energy losses, which include excitation of hybridized DPPs (HDPPs), we have found for N = 3 that the middle HDPP eigenfrequency is not excited in the middle layer due to symmetry constraint, whereas the excitation of the lowest HDPP eigenfrequency produces a Fano resonance in the graphene layer that is first traversed by the charged particle. While the angular distribution of transition radiation emitted in the far field region also shows asymmetry with respect to the traversal order by the incident charged particle at supra-THz frequencies, the integrated radiative energy loss is surprisingly independent of both d and N for N ≤ 5, which is explained by a dominant role of the outer graphene layers in transition radiation. We have further found that the integrated ohmic energy loss in optically thin MLG scales as ∝1/N at sub-THz frequencies, which is explained by exposing the role of dissipative processes in graphene at low frequencies. Finally, prominent peaks are observed at supra-THz frequencies in the integrated ohmic energy loss for MLG structures that are not optically thin. The magnitude of those peaks is found to scale with N for N ≥ 2, while their shape and position replicate the peak in a double-layer graphene (N = 2), which is explained by arguing that plasmon hybridization in such MLG structures is dominated by electromagnetic interaction between the nearest-neighbor graphene layers.

  3. Pressure Drop Test of Hybrid Mixing Vane Spacer Grid

    Energy Technology Data Exchange (ETDEWEB)

    Oh, D. S.; Chang, S. K.; Kim, B. D.; Chun, S. Y.; Chun, T. H

    2007-08-15

    The pressure loss test has been accomplished in the test section containing 5x5 rod bundle with a length of 2 m including 3 spacer grids. The test has been performed for the 5 kinds of spacer grids to compare the pressure loss characteristics: 1. Plain spacer grid which has the same body of the Hybrid but without vane (Plain), 2. Hybrid Vane spacer grid (Hybrid), 3. Hybrid-SC spacer grid which is constructed with coined, chamfered strip and is fabricated by spot welding, 4. Hybrid-LC spacer grid which is constructed with coined, chamfered strip and is fabricated by line welding along intersection line, 5. Westinghouse spacer grid with split vane (Plus-7). The pressure loss coefficient of the Plain, Hybrid, Hybrid-SC, Hybrid-LC, and Plus-7 spacer grid is 0.93, 1.15, 1.02, 1.04, and 1.08, respectively.

  4. GISH and AFLP analyses of novel Brassica napus lines derived from one hybrid between B. napus and Orychophragmus violaceus.

    Science.gov (United States)

    Ma, Ni; Li, Zai-Yun; Cartagena, J A; Fukui, K

    2006-10-01

    New Brassica napus inbred lines with different petal colors and with canola quality and increased levels of oleic (approximately 70%, 10% higher than that of B. napus parent) and linoleic (28%) acids have been developed in the progenies of one B. napus cv. Oro x Orychophragmus violaceus F5 hybrid plant (2n = 31). Their genetic constituents were analyzed by using the methods of genomic in situ hybridization (GISH) and amplified fragments length polymorphism (AFLP). No intact chromosomes of O. violaceus origin were detected by GISH in their somatic cells of ovaries and root tips (2n = 38) and pollen mother cells (PMCs) with normal chromosome pairing (19 bivalents) and segregation (19:19), though signals of variable sizes and intensities were located mainly at terminal and centromeric parts of some mitotic chromosomes and meiotic bivalents at diakinesis or chromosomes in anaphase I groups and one large patch of chromatin was intensively labeled and separated spatially in some telophase I nuclei and metaphase II PMCs. AFLP analysis revealed that substantial genomic changes have occurred in these lines and O. violaceus-specific bands, deleted bands in 'Oro' and novel bands for two parents were detected. The possible mechanisms for these results were discussed.

  5. Response of BP cell lines to γ-radiation: evaluation of DNA repair and apoptosis

    International Nuclear Information System (INIS)

    Paris, F.E.; Martin, M.; Le Rhum, Y.; May, E.; Duriez, P; Shah, G.

    1997-01-01

    In the BP cell lines, mutation of p53 gene is associated with an increased radiosensitivity. In order to understand the relation between p53 and radiosensitivity, we looked at DNA repair and cell death. Unexpectedly, after radiation the mutated p53 cell line BPp- Tu and the wild type p53 cell line BPp- Tu cells, both ell lines died by the same non necrotic process: a programmed cell death independent of their p53 status. The cleavage of poly (ADP-ribose) polymerase (PARP) by an ICE-related protease is considered an early and critical event during apoptosis. The fate of PARP was monitored by Western extensively in the apoptotic BPp- Tu cells than in the BPp cells. This faster PARP cleavage might be linked to the increased radiosensitivity of the BPp- Tu cells. (authors)

  6. Cardiac hybrid imaging

    Energy Technology Data Exchange (ETDEWEB)

    Gaemperli, Oliver [University Hospital Zurich, Cardiac Imaging, Zurich (Switzerland); University Hospital Zurich, Nuclear Cardiology, Cardiovascular Center, Zurich (Switzerland); Kaufmann, Philipp A. [University Hospital Zurich, Cardiac Imaging, Zurich (Switzerland); Alkadhi, Hatem [University Hospital Zurich, Institute of Diagnostic and Interventional Radiology, Zurich (Switzerland)

    2014-05-15

    Hybrid cardiac single photon emission computed tomography (SPECT)/CT imaging allows combined assessment of anatomical and functional aspects of cardiac disease. In coronary artery disease (CAD), hybrid SPECT/CT imaging allows detection of coronary artery stenosis and myocardial perfusion abnormalities. The clinical value of hybrid imaging has been documented in several subsets of patients. In selected groups of patients, hybrid imaging improves the diagnostic accuracy to detect CAD compared to the single imaging techniques. Additionally, this approach facilitates functional interrogation of coronary stenoses and guidance with regard to revascularization procedures. Moreover, the anatomical information obtained from CT coronary angiography or coronary artery calcium scores (CACS) adds prognostic information over perfusion data from SPECT. The use of cardiac hybrid imaging has been favoured by the dissemination of dedicated hybrid systems and the release of dedicated image fusion software, which allow simple patient throughput for hybrid SPECT/CT studies. Further technological improvements such as more efficient detector technology to allow for low-radiation protocols, ultra-fast image acquisition and improved low-noise image reconstruction algorithms will be instrumental to further promote hybrid SPECT/CT in research and clinical practice. (orig.)

  7. Sensitivity to ionizing radiation and chemotherapeutic agents in gemcitabine-resistant human tumor cell lines

    International Nuclear Information System (INIS)

    Bree, Chris van; Kreder, Natasja Castro; Loves, Willem J.P.; Franken, Nicolaas A.P.; Peters, Godefridus J.; Haveman, Jaap

    2002-01-01

    Purpose: To determine cross-resistance to anti-tumor treatments in 2',2'difluorodeoxycytidine (dFdC, gemcitabine)-resistant human tumor cells. Methods and Materials: Human lung carcinoma cells SW-1573 (SWp) were made resistant to dFdC (SWg). Sensitivity to cisplatin (cDDP), paclitaxel, 5-fluorouracil (5-FU), methotrexate (MTX), cytarabine (ara-C), and dFdC was measured by a proliferation assay. Radiosensitivity and radioenhancement by dFdC of this cell panel and the human ovarian carcinoma cell line A2780 and its dFdC-resistant variant AG6000 were determined by clonogenic assay. Bivariate flowcytometry was performed to study cell cycle changes. Results: In the SWg, a complete deoxycytidine kinase (dCK) deficiency was found on mRNA and protein level. This was accompanied by a 10-fold decrease in dCK activity which resulted in the >1000-fold resistance to dFdC. Sensitivity to other anti-tumor drugs was not altered, except for ara-C (>100-fold resistance). Radiosensitivity was not altered in the dFdC-resistant cell lines SWg and AG6000. High concentrations (50-100 μM dFdC) induced radioenhancement in the dFdC-resistant cell lines similar to the radioenhancement obtained at lower concentrations (10 nM dFdC) in the parental lines. An early S-phase arrest was found in all cell lines after dFdC treatment where radioenhancement was achieved. Conclusions: In the dFdC-resistant lung tumor cell line SWg, the deficiency in dCK is related to the resistance to dFdC and ara-C. No cross-resistance was observed to other anti-tumor drugs used for the treatment in lung cancer. Sensitivity to ionizing radiation was not altered in two different dFdC-resistant cell lines. Resistance to dFdC does not eliminate the ability of dFdC to sensitize cells to radiation

  8. Establishment of a new human pleomorphic malignant fibrous histiocytoma cell line, FU-MFH-2: molecular cytogenetic characterization by multicolor fluorescence in situ hybridization and comparative genomic hybridization

    Directory of Open Access Journals (Sweden)

    Isayama Teruto

    2010-11-01

    Full Text Available Abstract Background Pleomorphic malignant fibrous histiocytoma (MFH is one of the most frequent malignant soft tissue tumors in adults. Despite the considerable amount of research on MFH cell lines, their characterization at a molecular cytogenetic level has not been extensively analyzed. Methods and results We established a new permanent human cell line, FU-MFH-2, from a metastatic pleomorphic MFH of a 72-year-old Japanese man, and applied multicolor fluorescence in situ hybridization (M-FISH, Urovysion™ FISH, and comparative genomic hybridization (CGH for the characterization of chromosomal aberrations. FU-MFH-2 cells were spindle or polygonal in shape with oval nuclei, and were successfully maintained in vitro for over 80 passages. The histological features of heterotransplanted tumors in severe combined immunodeficiency mice were essentially the same as those of the original tumor. Cytogenetic and M-FISH analyses displayed a hypotriploid karyotype with numerous structural aberrations. Urovysion™ FISH revealed a homozygous deletion of the p16INK4A locus on chromosome band 9p21. CGH analysis showed a high-level amplification of 9q31-q34, gains of 1p12-p34.3, 2p21, 2q11.2-q21, 3p, 4p, 6q22-qter, 8p11.2, 8q11.2-q21.1, 9q21-qter, 11q13, 12q24, 15q21-qter, 16p13, 17, 20, and X, and losses of 1q43-qter, 4q32-qter, 5q14-q23, 7q32-qter, 8p21-pter, 8q23, 9p21-pter, 10p11.2-p13, and 10q11.2-q22. Conclusion The FU-MFH-2 cell line will be a particularly useful model for studying molecular pathogenesis of human pleomorphic MFH.

  9. A high-resolution whole genome radiation hybrid map of human chromosome 17q22-q25.3 across the genes for GH and TK

    Energy Technology Data Exchange (ETDEWEB)

    Foster, J.W.; Schafer, A.J.; Critcher, R. [Univ. of Cambridge (United Kingdom)] [and others

    1996-04-15

    We have constructed a whole genome radiation hybrid (WG-RH) map across a region of human chromosome 17q, from growth hormone (GH) to thymidine kinase (TK). A panel of 128 WG-RH hybrid cell lines generated by X-irradiation and fusion has been tested for the retention of 39 sequence-tagged site (STS) markers by the polymerase chain reaction. This genome mapping technique has allowed the integration of existing VNTR and microsatellite markers with additional new markers and existing STS markers previously mapped to this region by other means. The WG-RH map includes eight expressed sequence tag (EST) and three anonymous markers developed for this study, together with 23 anonymous microsatellites and five existing ESTs. Analysis of these data resulted in a high-density comprehensive map across this region of the genome. A subset of these markers has been used to produce a framework map consisting of 20 loci ordered with odds greater than 1000:1. The markers are of sufficient density to build a YAC contig across this region based on marker content. We have developed sequence tags for both ends of a 2.1-Mb YAC and mapped these using the WG-RH panel, allowing a direct comparison of cRay{sub 6000} to physical distance. 31 refs., 3 figs., 2 tabs.

  10. Radiation studied on the internet. On-line radiation teaching materials

    International Nuclear Information System (INIS)

    Inoue, Hiroyoshi; Kagoshima, Mayumi; Yamasaki, Mariko

    2005-01-01

    In order to facilitate scientific understanding of radiation in Japan where social understanding has been already progressed, we developed Internet radiation teaching materials that can be utilized as off-school teaching materials or supplementary materials. The teaching materials of ''atomic structure and radiation'' and ''medical treatment and radiation'' were tried for 160 high school students and 59 junior high school students, respectively. More than 70% of the student answered that these teaching materials were effective when they understand radiation. (author)

  11. Hybrid simulation models of production networks

    CERN Document Server

    Kouikoglou, Vassilis S

    2001-01-01

    This book is concerned with a most important area of industrial production, that of analysis and optimization of production lines and networks using discrete-event models and simulation. The book introduces a novel approach that combines analytic models and discrete-event simulation. Unlike conventional piece-by-piece simulation, this method observes a reduced number of events between which the evolution of the system is tracked analytically. Using this hybrid approach, several models are developed for the analysis of production lines and networks. The hybrid approach combines speed and accuracy for exceptional analysis of most practical situations. A number of optimization problems, involving buffer design, workforce planning, and production control, are solved through the use of hybrid models.

  12. Ionizing radiation effects on the KG1A primitive hematopoietic cell line

    International Nuclear Information System (INIS)

    Clave, Emmanuel; Carosella, Edgardo D.; Gluckman, Eliane; Dubray, Bernard; Socie, Gerard

    1996-01-01

    Purpose: Better understanding of radiation-induced effects on the hematopoietic system is important in both the context of therapeutic intervention and accidental exposure. However, direct study of these effects on the hematopoietic stem cell pool is hampered by the small number of accessible cells. We, thus, studied radiation-induced effects on the KG1a stem cell line. Methods and Materials: We confirmed and extended the immunophenotype of KG1a with monoclonal antibodies, established a radiation survival curve, and quantified mRNAs by Northern blotting 30 min after 1, 2, and 3 Gy of ionizing radiation (IR) and followed for up to 48 h after a 3 Gy dose. Cell cycle status and apoptosis were assessed by fluorescent-activated cell sorter (FACS) analysis, cell morphology, and DNA fragmentation. Results: KG1a was found to be CD34+, CD7+, Thy1 low, CD38 low, lineage negative (neg), C-KITneg and HLA-DRneg, a phenotype consistent with a primitive hematopoietic origin. This immunophenotype was not altered by x-ray irradiation. The D 0 value was 1.75 Gy. We showed a time-dependent variation of c-jun mRNA expression with an early and transient dose-dependent induction followed by a second increase at 24 and 48 h: a biphasic dose-dependent variation of bcl-2 expression 30 min after irradiation with a reduction of mRNA level at 1 Gy, and a normalization at higher doses and stable levels of mRNA for c-fos, c-myc, G-CSF, GM-CSF, IL-6, TNF-α, TGF-β, and MIP-1α genes. Cell cycle analysis showed the absence of G1/S phase arrest, a point consistent with the absence of detection of P53 mRNA by Northern blot analysis. The dose-dependent G2/M phase arrest was not followed by significant apoptotic cell death. Conclusion: Taken together, this data indicates that radiation-induced cell death of KG1a, a cell line that has a relatively high D 0 value, does not seem to be the result of the apoptotic pathway but occurs subsequent to a G2/M phase arrest

  13. Development of eggplant hybrid cultivar ‘BATEM FILIZI’ and determination of yield performance

    Directory of Open Access Journals (Sweden)

    Hatice Filiz BOYACI

    2014-12-01

    Full Text Available Eggplant (Solanum melongena L. cultivation is an important part of vegetable production in Turkey. It is highly affected by abiotic stress factors such as low temperatures during growth and development. The present study was aimed to improve new hybrid varieties with high quality and fruit setting under undesirable environmental conditions in eggplant. The 39 inbred lines were developed from eggplant genepool by using pedigree breeding method at the Bati Akdeniz Agricultural Research Institute. They were characterized morphologically and evaluated for phylogenetic relationship among them. Selected seven lines were crossed each other to obtain F1 hybrids. Thirteen hybrids from these crosses were examined for their rate of heterosis and heterobeltiosis. These hybrids were not suitable for market demands although F1 hybrids including P350 as parental line had high heterosis rate. Oval fruit shaped P350 inbred line as female parent showed good performance and crossed with long fruit shaped P599 inbred line. The resultant F1 hybrid cultivar higher yielding, suitable for production under greenhouse and highly adaptable was registered with the name of BATEM FILIZI. It is recommended that this hybrid can be used in greenhouse production especially in single crop season.

  14. Calculation of single phase AC and monopolar DC hybrid corona effects

    International Nuclear Information System (INIS)

    Zhao, T.; Sebo, S.A.; Kasten, D.G.

    1996-01-01

    Operating a hybrid HVac and HVdc line is an option for increasing the efficiency of power transmission and overcoming the difficulties in obtaining a new right-of-way. This paper proposes a new calculation method for the study of hybrid line corona. The proposed method can be used to calculate dc corona losses and corona currents in dc or ac conductors for single phase ac and monopolar dc hybrid lines. Profiles of electric field strength and ion current density at ground level can be estimated. The effects of the presence of an energized ac conductor on dc conductor corona and dc voltage on ac conductor corona are included in the method. Full-scale and reduced-scale experiments were utilized to investigate the hybrid line corona effects. Verification of the proposed calculation method is given

  15. Lithium line radiation in turbulent edge plasmas: Effects of low and high frequency temperature fluctuations

    Science.gov (United States)

    Rosato, J.; Capes, H.; Catoire, F.; Kadomtsev, M. B.; Levashova, M. G.; Lisitsa, V. S.; Marandet, Y.; Rosmej, F. B.; Stamm, R.

    2011-08-01

    In lithium-wall-conditioned tokamaks, the line radiation due to the intrinsic impurities (Li/Li+/Li++) plays a significant role on the power balance. Calculations of the radiation losses are usually performed using a stationary collisional-radiative model, assuming constant values for the plasma parameters (Ne, Te,…). Such an approach is not suitable for turbulent plasmas where the various parameters are time-dependent. This is critical especially for the edge region, where the fluctuation rates can reach several tens of percents [e.g. J.A. Boedo, J. Nucl. Mater. 390-391 (2009) 29-37]. In this work, the role of turbulence on the radiated power is investigated with a statistical formalism. A special emphasis is devoted to the role of temperature fluctuations, successively for low-frequency fluctuations and in the general case where the characteristic turbulence frequencies can be comparable to the collisional and radiative rates.

  16. Hybrid recreation by reverse breeding in Arabidopsis thaliana.

    Science.gov (United States)

    Wijnker, Erik; Deurhof, Laurens; van de Belt, Jose; de Snoo, C Bastiaan; Blankestijn, Hetty; Becker, Frank; Ravi, Maruthachalam; Chan, Simon W L; van Dun, Kees; Lelivelt, Cilia L C; de Jong, Hans; Dirks, Rob; Keurentjes, Joost J B

    2014-04-01

    Hybrid crop varieties are traditionally produced by selecting and crossing parental lines to evaluate hybrid performance. Reverse breeding allows doing the opposite: selecting uncharacterized heterozygotes and generating parental lines from them. With these, the selected heterozygotes can be recreated as F1 hybrids, greatly increasing the number of hybrids that can be screened in breeding programs. Key to reverse breeding is the suppression of meiotic crossovers in a hybrid plant to ensure the transmission of nonrecombinant chromosomes to haploid gametes. These gametes are subsequently regenerated as doubled-haploid (DH) offspring. Each DH carries combinations of its parental chromosomes, and complementing pairs can be crossed to reconstitute the initial hybrid. Achiasmatic meiosis and haploid generation result in uncommon phenotypes among offspring owing to chromosome number variation. We describe how these features can be dealt with during a reverse-breeding experiment, which can be completed in six generations (∼1 year).

  17. Analytic calculation of radiative-recoil corrections to muonium hyperfine splitting: Electron-line contribution

    International Nuclear Information System (INIS)

    Eides, M.I.; Karshenboim, S.G.; Shelyuto, V.A.

    1991-01-01

    The detailed account of analytic calculation of radiative-recoil correction to muonium hyperfine splitting, induced by electron-line radiative insertions, is presented. The consideration is performed in the framework of the effective two-particle formalism. A good deal of attention is paid to the problem of the divergence cancellation and the selection of graphs, relevant to radiative-recoil corrections. The analysis is greatly facilitated by use of the Fried-Yennie gauge for radiative photons. The obtained set of graphs turns out to be gauge-invariant and actual calculations are performed in the Feynman gauge. The main technical tricks, with the help of which we have effectively utilized the existence in the problem of the small parameter-mass ratio and managed to perform all calculations in the analytic form are described. The main intermediate results, as well as the final answer, δE rr = (α(Ζα)/π 2 )(m/M)E F (6ζ(3) + 3π 2 In 2 + π 2 /2 + 17/8), are also presented

  18. Andrographolide radiosensitizes human esophageal cancer cell line ECA109 to radiation in vitro.

    Science.gov (United States)

    Wang, Z-M; Kang, Y-H; Yang, X; Wang, J-F; Zhang, Q; Yang, B-X; Zhao, K-L; Xu, L-P; Yang, L-P; Ma, J-X; Huang, G-H; Cai, J; Sun, X-C

    2016-01-01

    To explore the radiosensitivity of andrographolide on esophageal cancer cell line ECA109. The inhibition effects of andrographolide were measured using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium (MTT) assay. Clonogenic survival assay was used to evaluate the effects of andrographolide on the radiosensitivity of esophageal cancer cells. Immunofluorescence was employed to examine Bax expression. The changes in cell cycle distribution and apoptosis were assayed using flow cytometry. The expression of NF-κb/Cleaved-Caspase3/Bax/Bcl-2 was measured using Western blot analysis. DNA damage was detected via γ-H2AX foci counting. With a clear dose and time effects, andrographolide was found to inhibit the proliferation of esophageal cell line ECA109. The results of the clonogenic survival assay show that andrographolide could markedly enhance radiosensitivity (P Andrographolide caused a dose-dependent increase in Cleaved-Caspase3/Bax protein expression and a decrease in Bcl-2/NF-κb expression. Apoptosis in andrographolide-treated ECA-109 increased significantly compared with the apoptosis in the simple drug and radiation combined with drug groups (P andrographolide combined with radiation group increased the number of DNA double chain breaks. Andrographolide can increase the radiosensitivity of esophageal cell line ECA109. This result may be associated with the decrease in the NF-κb level and the induced apoptosis of esophageal cancer cells. © 2014 International Society for Diseases of the Esophagus.

  19. Principle, function, experiences, hybrid chilled ceilings; Prinzip, Funktion, Erfahrungen. Hybrid-Kuehldecken

    Energy Technology Data Exchange (ETDEWEB)

    Schulz, Uwe W. [Hochschule Luzern (Switzerland). Technik und Architektur

    2010-12-15

    Hybrid chilled ceilings combine a radiation chilled ceiling with thermal activations of a concrete ceiling. The activation permits the utilization of alternative and/or small cold generators for the efficient night cooling. The traditional chilled ceiling grants a speedy reaction to load variations as well as a reduction of the reverberation periods. In addition, the combination supplies a draught-free insertion of air. Beside established solutions, MWH Barcol-Air (Staefa, Switzerland) also offers project-specific hybrid chilled ceilings.

  20. PV-Diesel Hybrid SCADA Experiment Network Design

    Science.gov (United States)

    Kalu, Alex; Durand, S.; Emrich, Carol; Ventre, G.; Wilson, W.; Acosta, R.

    1999-01-01

    The essential features of an experimental network for renewable power system satellite based supervisory, control and data acquisition (SCADA) are communication links, controllers, diagnostic equipment and a hybrid power system. Required components for implementing the network consist of two satellite ground stations, to satellite modems, two 486 PCs, two telephone receivers, two telephone modems, two analog telephone lines, one digital telephone line, a hybrid-power system equipped with controller and a satellite spacecraft. In the technology verification experiment (TVE) conducted by Savannah State University and Florida Solar Energy Center, the renewable energy hybrid system is the Apex-1000 Mini-Hybrid which is equipped with NGC3188 for user interface and remote control and the NGC2010 for monitoring and basic control tasks. This power system is connected to a satellite modem via a smart interface, RS232. Commands are sent to the power system control unit through a control PC designed as PC1. PC1 is thus connected to a satellite model through RS232. A second PC, designated PC2, the diagnostic PC is connected to both satellite modems via separate analog telephone lines for checking modems'health. PC2 is also connected to PC1 via a telephone line. Due to the unavailability of a second ground station for the ACTS, one ground station is used to serve both the sending and receiving functions in this experiment. Signal is sent from the control PC to the Hybrid system at a frequency f(sub 1), different from f(sub 2), the signal from the hybrid system to the control PC. f(sub l) and f(sub 2) are sufficiently separated to avoid interference.

  1. Radiative effect of black carbon aerosol on a squall line case in North China

    Science.gov (United States)

    Fu, Shizuo; Deng, Xin; Li, Zhe; Xue, Huiwen

    2017-11-01

    The radiative effect of black carbon aerosol (BC) on a squall line case in north China is studied with the Weather Research and Forecasting model. Before the initiation of the squall line, the surface-emitted BC is mixed only in the boundary layer (BL). BC is then transported from the BL into the free troposphere by the updrafts in the squall line system. Once distributed in the atmosphere, BC absorbs solar radiation and heats the surrounding air. The maximum increase of temperature is 0.05 K for the moderately polluted case bc2 and 0.37 K for the heavily polluted case bc20. In case bc2, where the BC concentration is not very high, the solar flux reaching the surface, the sensible heat flux, and the latent heat flux are not significantly affected by BC. In case bc20, the solar flux reaching the surface, the sensible heat flux, and the latent heat flux are reduced by up to 80, 30, and 21 W m- 2, respectively. The reduced surface evaporation leads to a reduced vapor amount at the early stage. After some time, the heating effect causes a large-scale convergence and brings slightly more vapor into the domain. The effect of BC on the cold pool strength and low-level wind shear is small and hence does not significantly affect the triggering of new convections. In addition, our results show that the effect of BC is negligible on the strength and rain rate of the squall line case.

  2. Radiation hybrid mapping of genes in the lithium-sensitive wnt signaling pathway.

    Science.gov (United States)

    Rhoads, A R; Karkera, J D; Detera-Wadleigh, S D

    1999-09-01

    Lithium, an effective drug in the treatment of bipolar disorder, has been proposed to disrupt the Wnt signaling pathway. To facilitate analysis of the possible involvement of elements of the Wnt pathway in human bipolar disorder, a high resolution radiation hybrid mapping (RHM) of these genes was performed. A fine physical location has been obtained for Wnt 7A, frizzled 3, 4 and 5, dishevelled 1, 2 and 3, GSK3beta, axin, alpha-catenin, the Armadillo repeat-containing genes (delta-catenin and ARVCF), and a frizzled-like protein (frpHE) using the Stanford Human Genome Center (SHGC) G3 panel. Most of these genes were previously mapped by fluorescence in situ hybridization (FISH). Frizzled 4, axin and frpHE did not have a previous chromosomal assignment and were linked by RHM to chromosome markers, SHGC-35131 at 11q22.1, NIB1488 at 16p13.3 and D7S2919 at 7p15.2, respectively. Interestingly, some of these genes were found to map within potential regions underlying susceptibility to bipolar disorder and schizophrenia as well as disorders of neurodevelopmental origin. This alternative approach of establishing the precise location of selected genetic components of a candidate pathway and determining if they map within previously defined susceptibility loci should help to identify plausible candidate genes that warrant further analysis through association and mutational scanning.

  3. Lower-hybrid heating and current drive on PLT

    International Nuclear Information System (INIS)

    Hooke, W.; Bernabei, S.; Boyd, D.

    1983-02-01

    Steady currents up to 165 kA for 3.5 seconds and 420 kA for 0.3 seconds have been maintained by 800 MHz lower hybrid waves. For line-averaged densities up to 7 x 10 12 cm - 3 the current is maintained with no input power from the ohmic heating transformer. The waves are launched with an array of six waveguides. Measurements of X rays and electron cyclotron radiation show that the rf power produces and maintains a suprathermal tail of electrons apparently independent of the number of fast electrons in the plasma prior to turning on the rf power. Measurements of current-drive efficiency and the electron tail provide direct evidence for a resonant wave-particle interaction. The radial profile of the rf-sustained current inferred from x-ray measurements is peaked in the center of the plasma and appears to obey the same q-value restraints as the inductively driven ohmic heating current. Current drive is observed to be accompanied always by radiation at frequencies greater than or equal to #betta#/sub ce/ and less than or equal to #betta#/sub pe/. The connection between this radiation and the current-drive mechanism is under study

  4. Fried-Yennie gauge recalculation of the electron line induced radiative-recoil corrections to muonium hyperfine splitting

    International Nuclear Information System (INIS)

    Brook, V.Yu.; Eides, M.I.; Karshenboim, S.G.; Shelyuto, V.A.

    1989-01-01

    A new analytic calculation of radiative-recoil corrections to muonium ground-state hyperfine splitting induced by electron line insertions is performed. The starting point of this calculation is presented by the Fried-Yennie gauge expression for the electron line factor. The final result confirms the one obtained previously from the apparently different expression in the Feynman gauge and removes the slight discrepancy which existed in the literature between the calculations in different gauges. (orig.)

  5. Nanostructure of PDMS–TEOS–PrZr hybrids prepared by direct deposition of gamma radiation energy

    International Nuclear Information System (INIS)

    Lancastre, Joana J.H.; Falcão, António N.; Margaça, Fernanda M.A.; Ferreira, Luís M.; Miranda Salvado, Isabel M.; Almásy, László; Casimiro, Maria H.; Meiszterics, Anikó

    2015-01-01

    Highlights: • Hybrid materials were prepared by direct energy deposition. • The influence of the catalyst content (PrZr) was investigated. • The developed oxide network was found to be strongly dependent on the PrZr content. • A model is proposed for the development of the oxide network in these materials. - Abstract: Organic–inorganic materials have been the object of intense research due to their wide range of properties and therefore innumerous applications. We prepared organic–inorganic hybrid materials by direct energy deposition on a mixture of polydimethylsiloxane silanol terminated (33 wt% fixed content), tetraethylorthosilicate and a minor content of zirconium propoxide that varied from 1 to 5 wt% using gamma radiation from a Co-60 source. The samples, dried in air at room temperature, are bulk, flexible and transparent. Their nanostructure was investigated by small angle neutron scattering. It was found that the inorganic oxide network has fractal structure, which becomes denser as the zirconium propoxide content decreases. The results suggest that oxide nanosized regions grow from the OH terminal group of PDMS which are the condensation seeds. Their number and position remains unaltered with the variation of zirconium propoxide content that only affects their microstructure. A model is proposed for the nanostructure of the oxide network that develops in the irradiation processed hybrid materials.

  6. Nanostructure of PDMS–TEOS–PrZr hybrids prepared by direct deposition of gamma radiation energy

    Energy Technology Data Exchange (ETDEWEB)

    Lancastre, Joana J.H., E-mail: jlancastre@ctn.ist.utl.pt [C2TN, Instituto Superior Técnico, Universidade de Lisboa, E.N. 10 (km 139.7), 2695-066 Bobadela, LRS (Portugal); Falcão, António N. [C2TN, Instituto Superior Técnico, Universidade de Lisboa, E.N. 10 (km 139.7), 2695-066 Bobadela, LRS (Portugal); Margaça, Fernanda M.A., E-mail: fmargaca@ctn.ist.utl.pt [C2TN, Instituto Superior Técnico, Universidade de Lisboa, E.N. 10 (km 139.7), 2695-066 Bobadela, LRS (Portugal); Ferreira, Luís M. [C2TN, Instituto Superior Técnico, Universidade de Lisboa, E.N. 10 (km 139.7), 2695-066 Bobadela, LRS (Portugal); Miranda Salvado, Isabel M. [CICECO & Departamento de Engenharia de Materiais e Cerâmica, Universidade de Aveiro, Campus de Santiago, 3810-193 Aveiro (Portugal); Almásy, László [Wigner Research Centre for Physics, Institute for Solid State Physics and Optics, PO Box 49, 1525 Budapest (Hungary); Casimiro, Maria H. [REQUIMTE/CQFB, Departamento de Química, Faculdade de Ciências e Tecnologia, FCT, Universidade Nova de Lisboa, 2829-516 Caparica (Portugal); Meiszterics, Anikó [Gedeon Richter Ltd., PO Box 27, H-1475 Budapest (Hungary)

    2015-10-15

    Highlights: • Hybrid materials were prepared by direct energy deposition. • The influence of the catalyst content (PrZr) was investigated. • The developed oxide network was found to be strongly dependent on the PrZr content. • A model is proposed for the development of the oxide network in these materials. - Abstract: Organic–inorganic materials have been the object of intense research due to their wide range of properties and therefore innumerous applications. We prepared organic–inorganic hybrid materials by direct energy deposition on a mixture of polydimethylsiloxane silanol terminated (33 wt% fixed content), tetraethylorthosilicate and a minor content of zirconium propoxide that varied from 1 to 5 wt% using gamma radiation from a Co-60 source. The samples, dried in air at room temperature, are bulk, flexible and transparent. Their nanostructure was investigated by small angle neutron scattering. It was found that the inorganic oxide network has fractal structure, which becomes denser as the zirconium propoxide content decreases. The results suggest that oxide nanosized regions grow from the OH terminal group of PDMS which are the condensation seeds. Their number and position remains unaltered with the variation of zirconium propoxide content that only affects their microstructure. A model is proposed for the nanostructure of the oxide network that develops in the irradiation processed hybrid materials.

  7. Lithium line radiation in turbulent edge plasmas: Effects of low and high frequency temperature fluctuations

    Energy Technology Data Exchange (ETDEWEB)

    Rosato, J., E-mail: joel.rosato@univ-provence.fr [PIIM, UMR 6633, Universite de Provence/CNRS, Centre de St.-Jerome, Case 232, F-13397 Marseille Cedex 20 (France); Capes, H.; Catoire, F. [PIIM, UMR 6633, Universite de Provence/CNRS, Centre de St.-Jerome, Case 232, F-13397 Marseille Cedex 20 (France); Kadomtsev, M.B.; Levashova, M.G.; Lisitsa, V.S. [ITP, Russian Research Center ' Kurchatov Institute' , Moscow (Russian Federation); Marandet, Y. [PIIM, UMR 6633, Universite de Provence/CNRS, Centre de St.-Jerome, Case 232, F-13397 Marseille Cedex 20 (France); Rosmej, F.B. [LULI, UMR 7605, Universite Pierre et Marie Curie/CNRS, 4 Place Jussieu, Case 128, F-75252 Paris Cedex 05 (France); Stamm, R. [PIIM, UMR 6633, Universite de Provence/CNRS, Centre de St.-Jerome, Case 232, F-13397 Marseille Cedex 20 (France)

    2011-08-01

    In lithium-wall-conditioned tokamaks, the line radiation due to the intrinsic impurities (Li/Li{sup +}/Li{sup ++}) plays a significant role on the power balance. Calculations of the radiation losses are usually performed using a stationary collisional-radiative model, assuming constant values for the plasma parameters (N{sub e}, T{sub e},...). Such an approach is not suitable for turbulent plasmas where the various parameters are time-dependent. This is critical especially for the edge region, where the fluctuation rates can reach several tens of percents [e.g. J.A. Boedo, J. Nucl. Mater. 390-391 (2009) 29-37]. In this work, the role of turbulence on the radiated power is investigated with a statistical formalism. A special emphasis is devoted to the role of temperature fluctuations, successively for low-frequency fluctuations and in the general case where the characteristic turbulence frequencies can be comparable to the collisional and radiative rates.

  8. A new hybrid support vector machine–wavelet transform approach for estimation of horizontal global solar radiation

    International Nuclear Information System (INIS)

    Mohammadi, Kasra; Shamshirband, Shahaboddin; Tong, Chong Wen; Arif, Muhammad; Petković, Dalibor; Ch, Sudheer

    2015-01-01

    Highlights: • Horizontal global solar radiation (HGSR) is predicted based on a new hybrid approach. • Support Vector Machines and Wavelet Transform algorithm (SVM–WT) are combined. • Different sets of meteorological elements are used to predict HGSR. • The precision of SVM–WT is assessed thoroughly against ANN, GP and ARMA. • SVM–WT would be an appealing approach to predict HGSR and outperforms others. - Abstract: In this paper, a new hybrid approach by combining the Support Vector Machine (SVM) with Wavelet Transform (WT) algorithm is developed to predict horizontal global solar radiation. The predictions are conducted on both daily and monthly mean scales for an Iranian coastal city. The proposed SVM–WT method is compared against other existing techniques to demonstrate its efficiency and viability. Three different sets of parameters are served as inputs to establish three models. The results indicate that the model using relative sunshine duration, difference between air temperatures, relative humidity, average temperature and extraterrestrial solar radiation as inputs shows higher performance than other models. The statistical analysis demonstrates that SVM–WT approach enjoys very good performance and outperforms other approaches. For the best SVM–WT model, the obtained statistical indicators of mean absolute percentage error, mean absolute bias error, root mean square error, relative root mean square error and coefficient of determination for daily estimation are 6.9996%, 0.8405 MJ/m 2 , 1.4245 MJ/m 2 , 7.9467% and 0.9086, respectively. Also, for monthly mean estimation the values are 3.2601%, 0.5104 MJ/m 2 , 0.6618 MJ/m 2 , 3.6935% and 0.9742, respectively. Based upon relative percentage error, for the best SVM–WT model, 88.70% of daily predictions fall within the acceptable range of −10% to +10%

  9. Magnetospheric line radiation: 6.5 years of observations by the DEMETER spacecraft

    Czech Academy of Sciences Publication Activity Database

    Bezděková, B.; Němec, F.; Parrot, M.; Santolík, Ondřej; Krupařová, Oksana

    2015-01-01

    Roč. 120, č. 11 (2015), s. 9442-9456 ISSN 2169-9380 R&D Projects: GA ČR(CZ) GAP209/11/2280; GA ČR(CZ) GA14-31899S; GA ČR GP13-37174P; GA MŠk LH12231 Institutional support: RVO:68378289 Keywords : magnetospheric line radiation * MLR * DEMETER Subject RIV: BL - Plasma and Gas Discharge Physics Impact factor: 3.318, year: 2015 http://onlinelibrary.wiley.com/doi/10.1002/2015JA021246/full

  10. Enhanced sensitivity of the RET proto-oncogene to ionizing radiation in vitro.

    Science.gov (United States)

    Volpato, Claudia Béu; Martínez-Alfaro, Minerva; Corvi, Raffaella; Gabus, Coralie; Sauvaigo, Sylvie; Ferrari, Pietro; Bonora, Elena; De Grandi, Alessandro; Romeo, Giovanni

    2008-11-01

    Exposure to ionizing radiation is a well-known risk factor for a number of human cancers, including leukemia and thyroid cancer. It has been known for a long time that exposure of cells to radiation results in extensive DNA damage; however, a small number of studies have tried to explain the mechanisms of radiation-induced carcinogenesis. The high prevalence of RET/PTC rearrangements in patients who have received external radiation, and the evidence of in vitro induction of RET rearrangements in human cells, suggest an enhanced sensitivity of the RET genomic region to damage by ionizing radiation. To assess whether RET is indeed more sensitive to radiations than other genomic regions, we used a COMET assay coupled with fluorescence in situ hybridization, which allows the measurement of DNA fragmentation in defined genomic regions of single cells. We compared the initial DNA damage of the genomic regions of RET, CXCL12/SDF1, ABL, MYC, PLA2G2A, p53, and JAK2 induced by ionizing radiation in both a lymphoblastoid and a fetal thyroid cell line. In both cell lines, RET fragmentation was significantly higher than in other genomic regions. Moreover, a differential distribution of signals within the COMET was associated with a higher percentage of RET fragments in the tail. RET was more susceptible to fragmentation in the thyroid-derived cells than in lymphoblasts. This enhanced susceptibility of RET to ionizing radiation suggests the possibility of using it as a radiation exposure marker.

  11. Synchronous Supraglottic and Esophageal Squamous Cell Carcinomas Treated with a Monoisocentric Hybrid Intensity-Modulated Radiation Technique

    Directory of Open Access Journals (Sweden)

    Christian L. Barney

    2018-01-01

    Full Text Available Risk factors for squamous cell carcinomas (SCCs of the head and neck (HN and esophagus are similar. As such, synchronous primary tumors in these areas are not entirely uncommon. Definitive chemoradiation (CRT is standard care for locally advanced HNSCC and is a preferred option for inoperable esophageal SCC. Simultaneous treatment of both primaries with CRT can present technical challenges. We report a case of synchronous supraglottic and esophageal SCC primary tumors, highlighting treatment with a monoisocentric hybrid radiation technique and normal tissue toxicity considerations.

  12. Molecular cytogenetic characterization of a human thyroid cancercell line

    Energy Technology Data Exchange (ETDEWEB)

    Weier, Heinz-Ulrich G.; Tuton, Tiffany B.; Ito, Yuko; Chu, LisaW.; Lu, Chung-Mei; Baumgartner, Adolf; Zitzelsberger, Horst F.; Weier,Jingly F.

    2006-01-04

    The incidence of papillary thyroid carcinoma (PTC) increases significantly after exposure of the head and neck region to ionizing radiation, yet we know neither the steps involved in malignant transformation of thyroid epithelium nor the specific carcinogenic mode of action of radiation. Such increased tumor frequency became most evident in children after the 1986 nuclear accident in Chernobyl, Ukraine. In the twelve years following the accident, the average incidence of childhood PTCs (chPTC) increased over one hundred-fold compared to the rate of about 1 tumor incidence per 10{sup 6} children per year prior to 1986. To study the etiology of radiation-induced thyroid cancer, we formed an international consortium to investigate chromosomal changes and altered gene expression in cases of post-Chernobyl chPTC. Our approach is based on karyotyping of primary cultures established from chPTC specimens, establishment of cell lines and studies of genotype-phenotype relationships through high resolution chromosome analysis, DNA/cDNA micro-array studies, and mouse xenografts that test for tumorigenicity. Here, we report the application of fluorescence in situ hybridization (FISH)-based techniques for the molecular cytogenetic characterization of a highly tumorigenic chPTC cell line, S48TK, and its subclones. Using chromosome 9 rearrangements as an example, we describe a new approach termed ''BAC-FISH'' to rapidly delineate chromosomal breakpoints, an important step towards a better understanding of the formation of translocations and their functional consequences.

  13. SU-E-T-320: The Effect of Survivin Perturbation On the Radiation Response of Breast Cancer Cell Lines

    International Nuclear Information System (INIS)

    Smith, D; Debeb, B; Woodward, W

    2014-01-01

    Purpose: Survivin is the smallest member of the inhibitor of apoptosis protein family and is well-known for its universal over-expression in human cancers. Due to its role in apoptosis and cellular proliferation, survivin is implicated in the radiation response in several cancer types, and antisurvivin treatments have had success as a radiation sensitizer in many preclinical cancer models. As no studies to date have reported survivin as a factor affecting radiation resistance in breast cancer models, we sought to evaluate the synergistic relationship between survivin function and irradiation in breast cancer cell lines. Methods: Information regarding survivin protein expression in breast cancer was retrieved from three public databases: Oncomine, Kaplan-Meier Plotter, and GOBO. For the in vitro studies, survivin function was compromised by transducing a non-functional mutant form (survivin-DN) into two breast cancer cell lines, the estrogen receptor-positive MCF7 and the triple-negative, inflammatory SUM149. Cell growth was compared in the survivin-DN and control populations with colony-formation assays. To assess how survivin affects radiation response, clonogenic assays were performed by irradiating the cell lines up to 6 Gy. Results: From the public databases, survivin is more highly expressed in triple-negative breast cancer compared to all other subtypes, and is prognostic of poor survival in all breast cancer patients. In MCF7, the survivin-DN population had decreased colony-formation potential; the opposite was true in SUM149. In the clonogenic assays, abrogation of survivin function radio-protected MCF7 cells in monolayer and 3D growth conditions, while SUM149 survivin-DN cells were radiosensitized in monolayer conditions. Conclusion: We observed synergy between survivin function and radiation, although the results between the two cell lines were disparate. Further investigation is required to identify the mechanism of this discrepancy, including evaluation

  14. Progress in application of hybrid numerical simulation methods to magnetic confinement systems. Annual report

    International Nuclear Information System (INIS)

    Morse, R.L.

    1979-06-01

    Hybrid codes have been developed to simulate high density, high β confined plasmas. The major areas of application have been end plugging and heating of linear confinement systems. In particular, significant progress has been made in understanding the role of line and recombination radiation in recent experiments which showed large increases in energy confinement times from the use of solid end plugs. Another accomplishment is the conception and theoretical analysis of an efficient, low frequency, axial heating method which we believe could significantly increase the attractiveness of linear systems as reactors

  15. Hybrid emergency radiation detection: a wireless sensor network application for consequence management of a radiological release

    Science.gov (United States)

    Kyker, Ronald D.; Berry, Nina; Stark, Doug; Nachtigal, Noel; Kershaw, Chris

    2004-08-01

    The Hybrid Emergency Radiation Detection (HERD) system is a rapidly deployable ad-hoc wireless sensor network for monitoring the radiation hazard associated with a radiation release. The system is designed for low power, small size, low cost, and rapid deployment in order to provide early notification and minimize exposure. The many design tradeoffs, decisions, and challenges in the implementation of this wireless sensor network design will be presented and compared to the commercial systems available. Our research in a scaleable modular architectural highlights the need and implementation of a system level approach that provides flexibility and adaptability for a variety of applications. This approach seeks to minimize power, provide mission specific specialization, and provide the capability to upgrade the system with the most recent technology advancements by encapsulation and modularity. The implementation of a low power, widely available Real Time Operating System (RTOS) for multitasking with an improvement in code maintenance, portability, and reuse will be presented. Finally future design enhancements technology trends affecting wireless sensor networks will be presented.

  16. Loss of a putative tumor suppressor locus after gamma-ray-induced neoplastic transformation of HeLa x Skin fibroblast human cell hybrids

    International Nuclear Information System (INIS)

    Mendonca, M.S.; Redpath, J.L.; Fasching, C.L.

    1995-01-01

    The nontumorigenic HeLa x skin fibroblast hybrid cell line, CGL1, can be induced to re-express HeLa tumor-associated cell surface antigen, p75-IAP (intestinal alkaline phosphatase), with resulting neoplastic transformation, by exposure to γ radiation. This has allowed the human hybrid system to be developed into a quantitative in vitro model for radiation-induced neoplastic transformation of human cells. Recently, several γ-ray-induced IAP-expression mutants (GIMs) of the nontumorigenic HeLa x skin fibroblast hybrid CGL1 were isolated and all were tumorigenic when injected subcutaneously into nude mice. Control cell lines which were negative for p75-IAP (CONs) were also isolated from irradiated populations, and none were found to be tumorigenic. We have now begun to investigate the molecular basis of radiation-induced neoplastic transformation in this system by studying the potential genetic linkage between p75/IAP expression, tumorigenicity and damage to a putative tumor suppressor locus on fibroblast chromosome 11. Previous analysis of rare spontaneous segregants has indicated that this locus is involved in the regulation of tumorigenicity and in the expression of the HeLa tumor-associated cell surface marker intestinal alkaline phosphatase (p75-IAP) in this system. Therefore, analysis by restriction fragment length polymorphism and chromosome painting have been performed for chromosome 11, and for chromosome 13 as a control, for the p75/IAP-positive GIM and p75/IAP-negative CON cell lines. We report that in five of eight of the GIMs large-scale damage to the fibroblast chromosome 11's is evident (four GIMs have lost one complete copy of a fibroblast chromosome 11 heavily damaged). None of the CONs, however (0/5), have lost a complete copy of either fibroblast chromosome 11. No large-scale damage to the control chromosome 13's was detected in the GIMs or CONs. 49 refs., 3 figs., 2 tabs

  17. Comparison of radiation sensitivity for three cell lines as measured by the cloning assay and the micro-nucleus test

    NARCIS (Netherlands)

    Stap, J.; Aten, J. A.

    1990-01-01

    The correlation between cell killing and the induction of micro-nuclei was studied for three cell lines after treatment with gamma radiation to investigate whether the frequency of micro-nucleated cells can be used to determine the radiation sensitivity of a cell type. R1 rat rhabdomyosarcoma cells

  18. A web based on-line radiation early warning system for emergency preparedness and response centre

    International Nuclear Information System (INIS)

    Bhujbal, Vaibhav; Saindane, Shashank S.; Narasaiah, M.V.R.; Murali, S.

    2018-01-01

    The topography of the Bhabha Atomic Research Centre, Trombay site, where all major components of nuclear fuel cycle activities are located is very complex in nature. The assessment of the radiological impact, if any, due to atmospheric releases from these facilities within BARC site is being carried out by using a Local Area Network (LAN) based Radiation Early Warning System and meteorological parameters. The upgraded system is aimed at providing data during normal operation of the various facilities at site and for providing early warning to decision makers in case of any onset of an emergency. It is carried out by acquiring both on-line and off-line data on releases from the plants, the environmental radiation dose rate at selected locations and other related parameters. The monitors placed at these selected locations including strategic point around the BARC site can also help in detecting any attempt of unauthorized trafficking of the radioactive sources. This paper explains different aspects of the system operating at BARC

  19. Analysis of Information Leakages on Laser Printers in the Media of Electromagnetic Radiation and Line Conductions

    OpenAIRE

    Ulaş, Cihan

    2016-01-01

    In this paper, the emissions of a laser printer, which may process classified information, are investigated in the media of electromagnetic radiation (ER), Power Line Conductors (PLC), and Signal Line Conductors (SLC). First, the candidate frequency points of CE are examined in the frequency domain. Second, the emitted signal is AM-demodulated with the proper bandwidth, and then sampled by a high storage oscilloscope in these frequency points. Third, the collected data is converted to 2D imag...

  20. In vitro oxygen-dependent survival of two human cell lines after combined radiations tirapazamin and cisplatin

    International Nuclear Information System (INIS)

    Lartigau, E.; Stern, S.; Guichard, M.

    2000-01-01

    Recent data have shown that the in vitro and in vivo cytotoxicity of bioreductive drugs could be significantly cytotoxicity of bioreductive drugs could be significantly increased by combination with ionising radiation or chemotherapy. Various parameters such as oxygen tension and timing of administration of the drugs could play a crucial role in the efficacy of combined treatment modalities. The aim of this study was to define the oxygen dependency of cell survival after in vitro irradiation and incubation with tirapazamin, a bioreductive drug, and cisplatin given alone or simultaneously. Two human cell lines were studied: one cell line sensitive to tirapazamin, Na11+, a pigmented melanoma with a high percentage of hypoxic cells, and a less sensitive cell line to tirapazamin, HRT18, a rectal adenocarcinoma. Gas changes were made to study cell survival at four different oxygen concentrations (pO 2 ): air (20.9 % O 2 ), 10.2 and 0.2 %. Cells were incubated with tirapazamin and cisplatin alone or combined for one hour at 37 deg C, then irradiated and cultured. For Na11+, cell survival after irradiation was comparable in air and at 10 % oxygen with the two drugs given alone or combined. At 2 and 0.2 % oxygen, cell killing was largely increased by tirapazamin and was not modified by the addition of cisplatin. For HRT18, cell survival was not modified when cisplatin was added to radiation, whatever the oxygen partial pressure. At low pO 2 (2 and 0.2 %) the cytotoxic effect of tirapazamin was not significantly decreased by the addition of cisplatin. When cytotoxic and bioreductive drugs are combined to radiation, the magnitude of the observed effect is highly dependent on the partial oxygen pressure and on the sensitivity of the cell line to the individual drugs. This has very important implications for clinical strategies based on combined chemo-radiotherapy. (authors)

  1. Cytotoxic effect and radiation enhancement of artemisinin in uterine cervical carcinoma cell line HeLa

    International Nuclear Information System (INIS)

    Gong Xiaomei; Zhou Daoan; Cao Jianping; Fan Saijun; Zhu Wei

    2010-01-01

    Objective: To investigate cytotoxic and radiosensitizing effect of Artemisinin on cervical carcinoma cell line HeLa. Methods: In order to measure the optimized effective time, cytotoxic effect of Artemisinin on HeLa cell line was investigated with MTT assay. The radiosensitization effect of different doses and different treatment duration of Artemisinin on HeLa cell line were evaluated by MTT test, the SER is 1.17 and radiosensitizing effect was measured with multi-target single hit model through SER of HeLa cell. Cell cycles in different groups were calculated by flow cytometry. Results: The 50% inhibition concentration of Artemisinin interacted with HeLa cells for 24 h is 600.19 nmol/ml, and for 48 h is 160.71 nmol/ml. The HeLa cells'surival ratio is 93.51%, 91.87%, and 87.28% after adding Atemisinin of 110.69 nmol/ml and 1 Gy radiation exposure. There are three groups: the chemotherapy only group, the radiotherapy only group and the combination group. The result of the cell cycles showed that cells in G 2 /M period decreased in the combination group. Conclusion: Artemisinin has radiosensitization effect on cervical carcinoma HeLa cells, whichshows dose and time dependent. Artemisinin can inhibit the G 2 /M block by ionizing radiation. (authors)

  2. Genetic and Epigenetic Alterations of Brassica nigra Introgression Lines from Somatic Hybridization: A Resource for Cauliflower Improvement.

    Science.gov (United States)

    Wang, Gui-Xiang; Lv, Jing; Zhang, Jie; Han, Shuo; Zong, Mei; Guo, Ning; Zeng, Xing-Ying; Zhang, Yue-Yun; Wang, You-Ping; Liu, Fan

    2016-01-01

    Broad phenotypic variations were obtained previously in derivatives from the asymmetric somatic hybridization of cauliflower "Korso" (Brassica oleracea var. botrytis, 2n = 18, CC genome) and black mustard "G1/1" (Brassica nigra, 2n = 16, BB genome). However, the mechanisms underlying these variations were unknown. In this study, 28 putative introgression lines (ILs) were pre-selected according to a series of morphological (leaf shape and color, plant height and branching, curd features, and flower traits) and physiological (black rot/club root resistance) characters. Multi-color fluorescence in situ hybridization revealed that these plants contained 18 chromosomes derived from "Korso." Molecular marker (65 simple sequence repeats and 77 amplified fragment length polymorphisms) analysis identified the presence of "G1/1" DNA segments (average 7.5%). Additionally, DNA profiling revealed many genetic and epigenetic differences among the ILs, including sequence alterations, deletions, and variation in patterns of cytosine methylation. The frequency of fragments lost (5.1%) was higher than presence of novel bands (1.4%), and the presence of fragments specific to Brassica carinata (BBCC 2n = 34) were common (average 15.5%). Methylation-sensitive amplified polymorphism analysis indicated that methylation changes were common and that hypermethylation (12.4%) was more frequent than hypomethylation (4.8%). Our results suggested that asymmetric somatic hybridization and alien DNA introgression induced genetic and epigenetic alterations. Thus, these ILs represent an important, novel germplasm resource for cauliflower improvement that can be mined for diverse traits of interest to breeders and researchers.

  3. Hybrid phase retrieval algorithm for solving the twin image problem in in-line digital holography

    Science.gov (United States)

    Zhao, Jie; Wang, Dayong; Zhang, Fucai; Wang, Yunxin

    2010-10-01

    For the reconstruction in the in-line digital holography, there are three terms overlapping with each other on the image plane, named the zero order term, the real image and the twin image respectively. The unwanted twin image degrades the real image seriously. A hybrid phase retrieval algorithm is presented to address this problem, which combines the advantages of two popular phase retrieval algorithms. One is the improved version of the universal iterative algorithm (UIA), called the phase flipping-based UIA (PFB-UIA). The key point of this algorithm is to flip the phase of the object iteratively. It is proved that the PFB-UIA is able to find the support of the complicated object. Another one is the Fienup algorithm, which is a kind of well-developed algorithm and uses the support of the object as the constraint among the iteration procedure. Thus, by following the Fienup algorithm immediately after the PFB-UIA, it is possible to produce the amplitude and the phase distributions of the object with high fidelity. The primary simulated results showed that the proposed algorithm is powerful for solving the twin image problem in the in-line digital holography.

  4. Evaluation of a polymerase chain reaction reverse hybridization line probe assay for the detection and identification of medically important fungi in bronchoalveolar lavage fluids.

    NARCIS (Netherlands)

    Meletiadis, J.; Melchers, W.J.G.; Meis, J.F.G.M.; Hurk, P.J.J.C. van den; Jannes, G.; Verweij, P.E.

    2003-01-01

    An assay system in which polymerase chain reaction (PCR) amplification of the ITS-1 region of ribosomal DNA (rDNA) is combined with a reverse-hybridization line probe assay (LiPA) was used for the identification of six Candida species and four Aspergillus species in pure cultures of clinical

  5. Effects of gamma radiation on the OM431 human ocular melanoma cell line

    International Nuclear Information System (INIS)

    Logani, S.; Cho, A.S.; Su, L.D.; Withers, H.R.; McBride, W.H.; Hall, M.O.; Lee, D.A.; Milani, J.K.; Straatsma, B.R.

    1995-01-01

    In order to determine the dose responsiveness to radiation of ocular melanoma, we conducted an in vitro dose-response study on a monolayer cell culture using a clonogenic assay. The effects on cell survival were determined relative to unirradiated controls. A human epithelioid ocular melanoma cell line, OM431, was maintained in tissue culture and serial dilutions of viable cells were plated in flasks, allowed to settle and attach for 48 h, and subsequently irradiated with 1-10 Gy in single fractions. After 2 weeks, the number of reproducing clones (forming colonies with greater than 32 cells or five generations) were counted. The surviving fractions of cells were plotted on a cell survival curve using the linear quadratic model. The survival curve showed a large initial shoulder followed by an exponential decline in growth. Our data suggest that the OM431 ocular melanoma cell line responds to irradiation in a manner similar to other melanoma cell lines and is relatively radioresistent especially at lower doses. (author)

  6. Radiation-induced apoptosis of chicken lymphocyte B-cell line DT40

    International Nuclear Information System (INIS)

    Furusawa, Y.; Aoki, M.; Takakura, K.

    2003-01-01

    Full text: Ionizing radiation causes lesions of DNA, cell cycle arrest, induced cell death, and apoptosis in the irradiated cells. Then it is easy to expect that those events would be increased in a cell line which is defective in DNA repair system. However, induction of apoptosis by irradiation takes so complicated process when the cells are defective of DNA repair system. Indeed by many recent studies it has been clarified that DNA repair gene is also concerned with apoptotic event and some study shows the contrary data. Thus, the relationship between the genetics of apoptosis and that of DNA repair is still unclear. In this study two kinds of DNA repair proteins, Rad54 and Ku70, were focused. Proteins of Rad54 and Ku70 have important role at two type of DNA repair systems called homologous recombination repair and non-homologous end joining repair, respectively. 4 phenotypes of DT40, parent type, ku70-/-, rad54-/- and ku70-/-/rad54-/- were used to study the radiation-induced apoptosis (Previous study shows that survival fraction of 4 phenotypes of DT40 is decreased in the cell line, in which DNA repair gene is defective). From the results in this study, two things are clarifies. One is that the dependence of apoptotic index on phenotypes is so different between at low dose and at high dose irradiation. The other is that Ku70 has effective role to induce apoptosis in DT40 irradiated with high dose X-rays

  7. Effects of hematin and Moessbauer gamma-radiation on human osteosarcoma cell lines

    International Nuclear Information System (INIS)

    Ortalli, I.; Pedrazzi, G.; Fano, V.; Ma, W.; Cai, S.; Giuliani, N.; Passeri, M.

    1996-01-01

    In the present work it is reported an investigation on cultures of the human osteosarcoma cell line 'MG-63' and the human osteoblastic cell line 'HOBIT'. It is examined the combined effects of hematin and 14,4keV gamma-radiation from a Moessbauer source. Preliminary results seem to confirm the trend already observed for the bone marrow system. Different degrees of growth inhibition were observed when hematin alone and hematin plus gamma-rays were administered to the cultures. It was previously found that hematin has no toxic effects on normal cells up to a concentration of 10 -4 M, but the same concentration produces various degrees of inhibition on cultures of tumor cells. While no significant effect could be attributed to irradiation alone, hematin plus irradiations show a larger inhibition than that expected for pure additive effects

  8. Hybrid particles and associated methods

    Science.gov (United States)

    Fox, Robert V; Rodriguez, Rene; Pak, Joshua J; Sun, Chivin

    2015-02-10

    Hybrid particles that comprise a coating surrounding a chalcopyrite material, the coating comprising a metal, a semiconductive material, or a polymer; a core comprising a chalcopyrite material and a shell comprising a functionalized chalcopyrite material, the shell enveloping the core; or a reaction product of a chalcopyrite material and at least one of a reagent, heat, and radiation. Methods of forming the hybrid particles are also disclosed.

  9. Persistence of translocations detected by means of fluorescence in situ hybridization in peripheral lymphocytes of accidentally exposed radiation workers

    International Nuclear Information System (INIS)

    Pressl, S.; Stephan, G.

    1997-01-01

    The translocation frequency in lymphocytes of radiation workers accidentally exposed a number of years earlier was determined by means of fluorescence in situ hybridization. Chromosomes 2, 4 and 8 were painted, and simultaneously the centromeres. The genomic frequency of translocations is between 1.7 and 17.9 per 1000 cells. This variation is not significantly different from the level in healthy control subjects. Therefore, no radiation exposure could be detected retrospectively. On the other hand, the frequency of dicentrics in these radiation workers measured by means of fluorescence plus Giemsa staining shortly after the exposure was significantly increased, and whole body doses between 0.2 and 0.3 Gy could be calculated. Consequently, it would seem that dicentrics measured shortly after an exposure are a more sensitive indicator than translocations which are determined years later. (author)

  10. Radiation induced expression of survivin in Ewing sarcoma cell-lines

    International Nuclear Information System (INIS)

    Sheikh-Mounessi, F.; Willich, N.; Greve, B.

    2009-01-01

    Full text: Introduction: Survivin belongs to the Inhibitor of Apoptosis Protein Family (IAP), is a protein of 16.5 kD and active as a homodimer. It is overexpressed in nearly all human tumors and has a vital function in cell division and apoptotic processes. Beside its role as a relevant prognostic and predictive factor it was described to be a molecular target to improve effectiveness of radiotherapy. We investigated the radiation induced survivin expression in Ewing sarcoma cell-lines. Methods: Ewing sarcoma cells were either irradiated with 10 Gy X-ray and harvested at different time points (0, 2, 4, 6, 10 and 24 h) or irradiated with different doses (0, 2, 5 and 10 Gy) and harvested 24 h later. Protein and mRNA expression was analysed by Westernblot or Real-Time PCR. Results: Directly after irradiation with 10 Gy X-ray survivin mRNA expression was increased in relation to the reference GAPDH. Protein expression was increased in a time dependent manner and reached a maximum after 24h. Three of four investigated cell-lines showed a significant dose dependent increase of survivin protein concentration 24h after irradiation. The same three cell-lines showed a LD50 of >30 Gy. The line with the lowest dose dependent survivin induction was investigated to be most radiosensitive (LD50 = 24 Gy). Discussion: Ewing sarcoma is a childhood tumor with relatively poor prognosis. This tumor often shows significant therapeutic resistance to chemo- and/or radiotherapy. It would be of high interest to find new therapeutic approaches for its treatment. We found a remarkable overexpression of survivin in untreated Ewing sarcoma and a time and dose dependent increase of survivin protein concentration after irradiation with X-ray. The cell-line with the lowest survivin induction showed the highest radiosensitivity. In conclusion, our results show that survivin is an inducible radioresistance factor in Ewing sarcoma. This may open new therapeutic options to treat this aggressive

  11. Regulation of radiation-induced protein kinase Cδ activation in radiation-induced apoptosis differs between radiosensitive and radioresistant mouse thymic lymphoma cell lines

    International Nuclear Information System (INIS)

    Nakajima, Tetsuo; Yukawa, Osami; Tsuji, Hideo; Ohyama, Harumi; Wang, Bing; Tatsumi, Kouichi; Hayata, Isamu; Hama-Inaba, Hiroko

    2006-01-01

    Protein kinase Cδ (PKCδ) has an important role in radiation-induced apoptosis. The expression and function of PKCδ in radiation-induced apoptosis were assessed in a radiation-sensitive mouse thymic lymphoma cell line, 3SBH5, and its radioresistant variant, XR223. Rottlerin, a PKCδ-specific inhibitor, completely abolished radiation-induced apoptosis in 3SBH5. Radiation-induced PKCδ activation correlated with the degradation of PKCδ, indicating that PKCδ activation through degradation is involved in radiation-induced apoptosis in radiosensitive 3SBH5. In radioresistant XR223, radiation-induced PKCδ activation was lower than that in radiosensitive 3SBH5. Cytosol PKCδ levels in 3SBH5 decreased markedly after irradiation, while those in XR223 did not. There was no apparent change after irradiation in the membrane fractions of either cell type. In addition, basal cytosol PKCδ levels in XR223 were higher than those in 3SBH5. These results suggest that the radioresistance in XR223 to radiation-induced apoptosis is due to a difference in the regulation of radiation-induced PKCδ activation compared to that of 3SBH5. On the other hand, Atm -/- mouse thymic lymphoma cells were more radioresistant to radiation-induced apoptosis than wild-type mouse thymic lymphoma cells. Irradiated wild-type cells, but not Atm -/- cells, had decreased PKCδ levels, indicating that the Atm protein is involved in radiation-induced apoptosis through the induction of PKCδ degradation. The decreased Atm protein levels induced by treatment with Atm small interfering RNA had no effect on radiation-induced apoptosis in 3SBH5 cells. These results suggest that the regulation of radiation-induced PKCδ activation, which is distinct from the Atm-mediated cascade, determines radiation sensitivity in radiosensitive 3SBH5 cells

  12. Propagation and radiation characteristics of the circular electric, circular magnetic and hybrid waveguide modes

    International Nuclear Information System (INIS)

    Crenn, J.P.

    1996-06-01

    The field distributions and propagation constants of the circular electric, circular magnetic and hybrid modes of oversized waveguides are expressed, taking the effects of walls into account. The near and far field patterns are derived in the case of real wall functions. It is shown that, for very oversized waveguides, the terms containing wall functions can be ignored in the calculations, and it results that the expressions of fields and propagation constants become independent of the types of waveguides. An application to corrugated waveguides for Electron Cyclotron Resonance Heating experiments shows the variations of the radiation characteristics versus geometric parameters of the corrugations and determines the ranges of interest for these parameters. (author)

  13. Lethality of radiation-induced chromosome aberrations in human tumour cell lines with different radiosensitivities.

    Science.gov (United States)

    Coco-Martin, J M; Ottenheim, C P; Bartelink, H; Begg, A C

    1996-03-01

    In order to find an explanation for the eventual disappearance of all chromosome aberrations in two radiosensitive human tumour cell lines, the type and stability of different aberration types was investigated in more detail. To classify the aberrations into unstable and stable types, three-colour fluorescence in situ hybridization was performed, including a whole-chromosome probe, a pancentromere probe, and a stain for total DNA. This technique enables the appropriate classification of the aberrations principally by the presence (stable) or not (unstable) of a single centromere per chromosome. Unstable-type aberrations were found to disappear within 7 days (several divisions) in the two radiosensitive and the two radioresistant tumour lines investigated. Stable-type aberrations were found to remain at an approximately constant level over the duration of the experiment (14 days; 8-10 divisions) in the two radioresistant lines. In contrast, the majority of these stable-type aberrations had disappeared by 14 days in the two radiosensitive lines. The previous findings of disappearance of total aberrations in radiosensitive cells was therefore not due to a reduced induction of stable-type aberrations, but the complete disappearance of cells with this aberration type. These results could not be explained by differences in apoptosis or G1 blocks. Two possible explanations for these unexpected findings involve non-random induction of unstable-type aberrations, or lethality of stable-type aberrations. The results suggest caution in the use of stable-type aberration numbers as a predictor for radiosensitivity.

  14. The tension adjuster used at the work line of electron beam radiation cross-linking wire and cable

    International Nuclear Information System (INIS)

    Zhang Yingfa; Liu Zhenhao; Yin Xuejun

    1999-01-01

    The tension adjuster is an important equipment in the transport system at the work line of electron beam radiation cross-linking wire and cable to realize the velocity synchronism. There are many kinds of the adjuster. By putting various adjusters together properly, the authors can keep the line work stable and raise the quality of the product. Two kinds of standing adjuster and their mechanism are introduced. Also the corresponding figures and formulas are shown

  15. Carboplatin- and cisplatin-induced potentiation of moderate-dose radiation cytotoxicity in human lung cancer cell lines

    NARCIS (Netherlands)

    Groen, H. J.; Sleijfer, S.; Meijer, C.; Kampinga, H. H.; Konings, A. W. T.; de Vries, E. G. E.; Mulder, N. H.

    1995-01-01

    The interaction between moderate-dose radiation and cisplatin or carboplatin was studied in a cisplatin-sensitive (GLC(4)) and -resistant (GLC(4)-CDDP) human small-cell lung cancer cell line. Cellular toxicity was analysed under oxic conditions with the microculture tetrazolium assay. For the

  16. A hybrid model of primary radiation damage in crystals

    International Nuclear Information System (INIS)

    Samarin, S.I.; Dremov, V.V.

    2009-01-01

    The paper offers a hybrid model which combines molecular dynamics and Monte Carlo (MD+MC) methods to describe primary radiation damage in crystals, caused by particles whose energies are no higher than several tens of keV. The particles are tracked in accord with equations of motion with account for pair interaction. The model also considers particle interaction with the mean-field potential (MFP) of the crystal. Only particles involved in cascading are tracked. Equations of motion for these particles include dissipative forces which describe energy exchange between cascade particles and electrons. New particles - the atoms of the crystal in the cascade region - have stochastic parameters (phase coordinates); they are sampled by the Monte Carlo method from the distribution that describes the classic canonical ensemble of non-interacting particles subjected to the external MFP. The introduction of particle interaction with the MFP helps avoid difficulties related to crystal stability and the choice of an adequate interparticle interaction potential in the traditional MD methods. Our technique is many times as fast as the traditional MD methods because we consider only particles which are involved in cascading and apply special methods to speedup the calculation of forces by accounting for the short-range pair potential used

  17. Adaptation of antenna profiles for control of MR guided hyperthermia (HT) in a hybrid MR-HT system

    International Nuclear Information System (INIS)

    Weihrauch, Mirko; Wust, Peter; Weiser, Martin; Nadobny, Jacek; Eisenhardt, Steffen; Budach, Volker; Gellermann, Johanna

    2007-01-01

    A combined numerical-experimental iterative procedure, based on the Gauss-Newton algorithm, has been developed for control of magnetic resonance (MR)-guided hyperthermia (HT) applications in a hybrid MR-HT system BSD 2000 3D-MRI. In this MR-HT system, composed of a 3-D HT applicator Sigma-Eye placed inside a tunnel-type MR tomograph Siemens MAGNETOM Symphony (1.5 T), the temperature rise due to the HT radiation can be measured on-line in three dimensions by use of the proton resonance frequency shift (PRFS) method. The basic idea of our iterative procedure is the improvement of the system's characterization by a step-by-step modification of the theoretical HT antenna profiles (electric fields radiated by single antennas). The adaptation of antenna profiles is efficient if the initial estimates are radiation fields calculated from a good a priori electromagnetic model. Throughout the iterative procedure, the calculated antenna fields (FDTD) are step-by-step modified by comparing the calculated and experimental data, the latter obtained using the PRFS method. The procedure has been experimentally tested on homogeneous and inhomogeneous phantoms. It is shown that only few comparison steps are necessary for obtaining a dramatic improvement of the general predictability and quality of the specific absorption rate (SAR) inside the MR-HT hybrid system

  18. 'Gornooriahovska Kapia F1' - A new hybrid pepper variety based on radiation induced male sterility

    International Nuclear Information System (INIS)

    Daskalov, S.

    2001-01-01

    The female parent line 'Zlaten medal ms-8' was obtained by Daskalov as a gamma rays induced mutant of pepper (Capsicum annum L.). Dry seeds of the initial variety 'Zlaten medal' were irradiated with 135 Gy gamma rays and after screening of a large M 2 population (57,000 plants) 3 male sterile mutants were obtained. After an allelic test the genes responsible for male sterility were denoted as ms-6, ms-7, and ms-8. After many years of testing various male sterility sources the gene ms-8 proved to be the most suitable for hybrid seed production because it determines 100% male sterility, independent of the climatic condition or the genotype. The mutation causes the highest reduction of anthers, which allows easy distinction between male sterile and fertile flowers. The development of the mutant male sterile line Zlaten medal ms-8 is a good example of how mutation techniques can be very useful for adding or changing only one trait without altering the basic genotype

  19. Life cycle assessment of hybrid vehicles recycling: Comparison of three business lines of dismantling.

    Science.gov (United States)

    Belboom, Sandra; Lewis, Grégory; Bareel, Pierre-François; Léonard, Angélique

    2016-04-01

    This paper undertakes an environmental evaluation of hybrid vehicles recycling, using industrial data from Comet Traitement SA in Belgium. Three business lines have been modelled and analysed. The first one is relative to the business as usual with a dismantling to recover batteries and engines followed by shredding and post shredding treatments. The second one considers, in addition, the removal of electronic control units (ECU) before shredding followed by same steps than in the first line and the last one is relative to the additional removal of big plastic parts before shredding and business as usual post shredding treatments. Results show non-significant environmental benefits when ECU or large parts of plastics are recovered before shredding. Improvements in terms of environmental benefits are lower than the uncertainty of the results. Indeed, the performing usual process for end-of-life vehicles (ELV) treatment reaches 97% of the ELV which is valorised in terms of metal and energy recoveries. Post shredding treatment units include metals, plastics and energy recovery of residues. Comet business as usual route for ELV valorisation is in accordance with the requirements of the European directive and recommendations for further improvement with dismantling of other parts (ECU or plastics) before shredding are non-relevant in this case. Copyright © 2016 Elsevier Ltd. All rights reserved.

  20. Genetic and epigenetic alterations of Brassica nigra introgression lines from somatic hybridization: a resource for cauliflower improvement

    Directory of Open Access Journals (Sweden)

    Guixiang Wang

    2016-08-01

    Full Text Available Broad phenotypic variations were obtained previously in derivatives from the asymmetric somatic hybridization of cauliflower ‘Korso’ (Brassica oleracea var. botrytis, 2n = 18, CC genome and black mustard ‘G1/1’ (Brassica nigra, 2n = 16, BB genome. However, the mechanisms underlying these variations were unknown. In this study, 28 putative introgression lines (ILs were pre-selected according to a series of morphological (leaf shape and color, plant height and branching, curd features, and flower traits and physiological (black rot/club root resistance characters. Multi-color fluorescence in situ hybridization revealed that these plants contained 18 chromosomes derived from ‘Korso’. Molecular marker (65 simple sequence repeats and 77 amplified fragment length polymorphisms analysis identified the presence of ‘G1/1’ DNA segments (average 7.5%. Additionally, DNA profiling revealed many genetic and epigenetic differences among the ILs, including sequence alterations, deletions, and variation in patterns of cytosine methylation. The frequency of fragments lost (5.1% was significantly higher than presence of novel bands (1.4%, and the presence of fragments specific to B. carinata (BBCC 2n = 34 were common (average 15.5%. Methylation-sensitive amplified polymorphism analysis indicated that methylation changes were common and that hypermethylation (12.4% was more frequent than hypomethylation (4.8%. Our results suggested that asymmetric somatic hybridization and alien DNA introgression induced genetic and epigenetic alterations. Thus, these ILs represent an important, novel germplasm resource for cauliflower improvement that can be mined for diverse traits of interest to breeders and researchers.

  1. Experimental Study of Coherent Synchrotron Radiation in the Emittance Exchange Line at the A0-Photoinjector

    Science.gov (United States)

    Thangaraj, Jayakar C. T.; Thurman-Keup, R.; Johnson, A.; Lumpkin, A. H.; Edwards, H.; Ruan, J.; Santucci, J.; Sun, Y. E.; Church, M.; Piot, P.

    2010-11-01

    Next generation accelerators will require a high current, low emittance beam with a low energy spread. Such accelerators will employ advanced beam conditioning systems such as emittance exchangers to manipulate high brightness beams. One of the goals of the Fermilab A0 photoinjector is to investigate the transverse to longitudinal emittance exchange principle. Coherent synchrotron radiation could limit high current operation of the emittance exchanger. In this paper, we report on the preliminary experimental and simulation study of the coherent synchroton radiation (CSR) in the emittance exchange line at the A0 photoinjector.

  2. Line photon transport in a non-homogeneous plasma using radiative coupling coefficients

    International Nuclear Information System (INIS)

    Florido, R.; Gil, J.M.; Rodriguez, R.; Rubiano, J.G.; Martel, P.; Florido, R.; Gil, J.M.; Rodriguez, R.; Rubiano, J.G.; Martel, P.; Minguez, E.

    2006-01-01

    We present a steady-state collisional-radiative model for the calculation of level populations in non-homogeneous plasmas with planar geometry. The line photon transport is taken into account following an angle- and frequency-averaged escape probability model. Several models where the same approach has been used can be found in the literature, but the main difference between our model and those ones is that the details of geometry are exactly treated in the definition of coupling coefficients and a local profile is taken into account in each plasma cell. (authors)

  3. Milk line as an indicator of the harvesting time of three hybrid seeds of corn (Zea mays L.

    Directory of Open Access Journals (Sweden)

    Célio Trzeciak dos Santos

    2005-03-01

    Full Text Available The experiment was carried out to evaluate the time for harvesting seeds of three hybrids of corn (Zea mays L. at their best physiological quality and the use of the milk line as an indicator of the physiological seed maturity. Single hybrids CD 1723 and CD 5501 and double hybrid OC 705 seeds were collected for 59 days, every four days, starting 23 days after female flowering. Seed dry weight, moisture content, germination, vigour (cold and accelerated aging tests, black layer formation and seed milk line development were analysed. The harvesting time started 47 days after female flowering with high physiological quality of the seeds identified by a joint analysis of those seven characteristics of them. The milk line at stage 4 proved to be the best indicator of the time to harvest corn seeds for maximum physiological quality, because different stages of milk line development in corn seeds could be easily identified in the filed without any special equipment.O experimento foi conduzido durante o ano agrícola de 1996/97 em área experimental da Cooperativa de Desenvolvimento Econômico e Tecnológico (Coodetec, em Cascavel, Paraná, com o objetivo de avaliar o efeito da época de colheita de três híbridos de milho (Zea mays L. na qualidade fisiológica das sementes e o uso da linha de solidificação do endosperma como indicativo da maturidade fisiológica das sementes. O plantio foi realizado em 30 de setembro de 1996. Amostras de sementes dos híbridos simples CD 1723 e CD 5501, bem como do híbrido duplo OC 705, foram colhidas em intervalos de quatro dias, durante 59 dias, iniciando-se a colheita no vigésimo terceiro dia após o florescimento feminino. As características avaliadas nas sementes foram o acúmulo de matéria seca, o conteúdo de umidade, a germinação, o vigor (pelos testes de frio e de envelhecimento acelerado, a formação da camada preta e o desenvolvimento da linha de solidificação do endosperma. A colheita dos tr

  4. Effect of an aminothiol (WR-1065) on radiation-induced mutagenesis and cytotoxicity in two repair-deficient mammalian cell lines

    International Nuclear Information System (INIS)

    Grdina, D.J.; Nagy, B.; Meechan, P.J.

    1991-01-01

    WR-2721 and its free thiol WR-1065 have been found to effectively protect against radiation- and/or chemotherapy-induced mutagenesis, transformation and carcinogenesis. With respect to the antimutagenic effect, WR-1065 significantly reduced the frequency of HGPRT mutants even when it was administered up to three hours following exposure of cells to radiation. The mechanisms of action most often attributed to these agents include their ability to scavenge free radicals, enter into chemical repair processes through the donation of hydrogen atoms, and induce intracellular hypoxia by means of auto-oxidative processes. Although evidence exists for each of these processes, none is sufficiently satisfactory to account for the post-irradiation protection of WR-1065 against mutation induction in mammalian cells. The most elegant work describing the role of aminothiols on cellular enzymatic repair processes has focused on well-characterized repair-proficient and -deficient bacterial and yeast cell systems. Protection against radiation-induced cytotoxicity by the aminothiol cysteamine was absent in E. coli cell lines that were characterized as having genetically defective repair systems. Until recently, such studies could not be effectively performed with mammalian cells. However, with the isolation and characterization of rodent cell lines deficient in their ability to repair DNA damage, it is now possible to investigate the role of cell-mediated repair systems on aminothiol radioprotection. Specifically, the authors have investigated the effects of WR-1065 on radiation-induced mutagenesis and cytotoxicity in cell lines EM9 and xrs-5, which are defective in DNA single-strand break (SSB) and double-strand break (DSB) rejoining, respectively. Corresponding parental repair-proficient cell lines, AA8 and K1, were also studied for comparative purposes. 26 refs., 5 figs., 2 tabs

  5. Analysis of chromosome aberration data by hybrid-scale models

    International Nuclear Information System (INIS)

    Indrawati, Iwiq; Kumazawa, Shigeru

    2000-02-01

    This paper presents a new methodology for analyzing data of chromosome aberrations, which is useful to understand the characteristics of dose-response relationships and to construct the calibration curves for the biological dosimetry. The hybrid scale of linear and logarithmic scales brings a particular plotting paper, where the normal section paper, two types of semi-log papers and the log-log paper are continuously connected. The hybrid-hybrid plotting paper may contain nine kinds of linear relationships, and these are conveniently called hybrid scale models. One can systematically select the best-fit model among the nine models by among the conditions for a straight line of data points. A biological interpretation is possible with some hybrid-scale models. In this report, the hybrid scale models were applied to separately reported data on chromosome aberrations in human lymphocytes as well as on chromosome breaks in Tradescantia. The results proved that the proposed models fit the data better than the linear-quadratic model, despite the demerit of the increased number of model parameters. We showed that the hybrid-hybrid model (both variables of dose and response using the hybrid scale) provides the best-fit straight lines to be used as the reliable and readable calibration curves of chromosome aberrations. (author)

  6. Radiation induced wheat-rye chromosomal translocations in triticale. Optimizing the dose using fluorescence in situ hybridization

    International Nuclear Information System (INIS)

    Ahmad, F.; Comeau, A.; Chen, Q.; Collin, J.; St-Pierre, C.A.

    2000-01-01

    Fluorescent in situ hybridization (FISH) was utilized to monitor the level of ionizing radiation ( 60 Co source) in their ability to cause intra- and intergeneric chromosomal aberrations in triticale seeds. Seeds were irradiated with 0, 20, 50, 100, 200, 300, 400, 500 and 1000 Gy doses. The root growth of irradiated seeds was greatly inhibited at 200 Gy and above. Various types of aberrations including wheat-rye, wheat-wheat, rye-rye, wheat-rye-wheat, rye-wheat-rye translocations and acentric fragments with or without translocations were observed. There was a consistent increase in proportion of aberrations per cell with an increase in radiation dose. It was concluded that for an optimal level of chromosomal translocation and least number of acentric fragments, a 20 Gy dose was quite sufficient for inducing a desirable level of wheat-rye chromosomal translocations. The excellent efficiency and importance of utilizing FISH in such studies of alien-introgression via chromosomal translocations are discussed. (author)

  7. Radiation induced wheat-rye chromosomal translocations in triticale. Optimizing the dose using fluorescence in situ hybridization

    Energy Technology Data Exchange (ETDEWEB)

    Ahmad, F. [Brandon Univ., Manitoba (Canada); Comeau, A.; Chen, Q.; Collin, J.; St-Pierre, C.A.

    2000-03-01

    Fluorescent in situ hybridization (FISH) was utilized to monitor the level of ionizing radiation ({sup 60}Co source) in their ability to cause intra- and intergeneric chromosomal aberrations in triticale seeds. Seeds were irradiated with 0, 20, 50, 100, 200, 300, 400, 500 and 1000 Gy doses. The root growth of irradiated seeds was greatly inhibited at 200 Gy and above. Various types of aberrations including wheat-rye, wheat-wheat, rye-rye, wheat-rye-wheat, rye-wheat-rye translocations and acentric fragments with or without translocations were observed. There was a consistent increase in proportion of aberrations per cell with an increase in radiation dose. It was concluded that for an optimal level of chromosomal translocation and least number of acentric fragments, a 20 Gy dose was quite sufficient for inducing a desirable level of wheat-rye chromosomal translocations. The excellent efficiency and importance of utilizing FISH in such studies of alien-introgression via chromosomal translocations are discussed. (author)

  8. Preparation of oligonucleotide microarray for radiation-associated gene expression detection and its application in lung cancer cell lines

    International Nuclear Information System (INIS)

    Guo Wanfeng; Lin Ruxian; Huang Jian; Guo Guozhen; Wang Shengqi

    2005-01-01

    Objective: The response of tumor cell to radiation is accompanied by complex change in patterns of gene expression. It is highly probable that a better understanding of molecular and genetic changes can help to sensitize the radioresistant tumor cells. Methods: Oligonucleotide microarray provides a powerful tool for high-throughput identifying a wider range of genes involved in the radioresistance. Therefore, the authors designed one oligonucleotide microarray according to the biological effect of IR. By using different radiosensitive lung cancer cell lines, the authors identified genes showing altered expression in lung cancer cell lines. To provide independent confirmation of microarray data, semi-quantitative RT-PCR was performed on a selection of genes. Results: In radioresistant A549 cell lines, a total of 18 genes were selected as having significant fold-changes compared to NCI-H446, 8 genes were up-regulated and 10 genes were down-regulated. Subsequently, A549 and NCI-H446 cells were delivered by ionizing radiation. In A549 cell line, we found 22 (19 up-regulated and 3 down-regulated) and 26 (8 up-regulated and 18 down-regulated) differentially expressed genes at 6h and 24h after ionizing radiation. In NCI-H446 cell line, we identified 17 (9 up-regulated and 8 down-regulated) and 18 (6 up-regulated and 12 down-regulated) differentially expressed genes at 6 h and 24 h after ionizing radiation. The authors tested seven genes (MDM2, p53, XRCC5, Bcl-2, PIM2, NFKBIA and Cyclin B1) for RT-PCR, and found that the results were in good agreement with those from the microarray data except for NFKBIA gene, even though the value for each mRNA level might be different between the two measurements. In present study, the authors identified some genes with cell proliferation and anti-apoptosis, such as MdM2, BCL-2, PKCz and PIM2 expression levels increased in A549 cells and decreased in NCI-H446 cells after radiation, and other genes with DNA repair, such as XRCC5, ERCC5

  9. Radiation of planar electromagnetic waves by a line source in anisotropic metamaterials

    International Nuclear Information System (INIS)

    Cheng Qiang; Jiang Weixiang; Cui Tiejun

    2010-01-01

    We show experimentally that a line source in an anisotropic metamaterial directly radiates planar electromagnetic waves instead of cylindrical waves, when one component of the permeability tensor approaches zero. The impedance of this material can be perfectly matched to that of free space, which can significantly reduce the reflections between the source and the superstrate, as in traditional highly directive antennas based on zero index metamaterials. Such a unique property determines the two-way propagation of electromagnetic waves excited by a line source, instead of all-way propagation. From this feature, a highly directive emission of electromagnetic waves is achieved using the anisotropic metamaterial with arbitrary shape. We have designed and fabricated the anisotropic metamaterial in the microwave region, and observed the generation of plane waves and their highly directive emission. The proposed plane-wave emission is independent of the shape variance of the anisotropic metamaterial, which can be utilized in the design of conformal antennas.

  10. Hybrid plasma system for magnetron deposition of coatings with ion assistance

    International Nuclear Information System (INIS)

    Vavilin, K V; Kralkina, E A; Nekludova, P A; Petrov, A. K; Nikonov, A M; Pavlov, V B; Airapetov, A A; Odinokov, V V; Pavlov, G Ya; Sologub, V A

    2016-01-01

    The results of the study of the plasma hybrid system based on the combined magnetron discharge and high-frequency inductive discharge located in the external magnetic field is presented. Magnetron discharge provides the generation of atoms and ions of the target materials while the flow of accelerated ions used for the ion assistance is provided by the RF inductive discharge. An external magnetic field is used to optimize the power input to the discharge, to increase the ion current density in the realm of substrate and to enhance the area of uniform plasma. The joint operation of magnetron and RF inductive discharge leads to a substantial increase (not equal to the sum of the parameters obtained under separate operation of two hybrid system channels) of the ion current density and intensity of sputtered material spectral lines radiation. Optimal mode of the hybrid plasma system operation provides uniform ion current density on the diameter of at least 150mm at 0.7PA argon pressure. The optimal values of the magnetic fields in the region of the substrate location lie in the range 2-8 mTl, while in the region of the RF input power unit lie in the range 0.5-25 mTl. (paper)

  11. GAMMA–GAMMA ABSORPTION IN THE BROAD LINE REGION RADIATION FIELDS OF GAMMA-RAY BLAZARS

    Energy Technology Data Exchange (ETDEWEB)

    Böttcher, Markus; Els, Paul, E-mail: Markus.Bottcher@nwu.ac.za [Centre for Space Research, North-West University, Potchefstroom, 2531 (South Africa)

    2016-04-20

    The expected level of γγ absorption in the Broad Line Region (BLR) radiation field of γ -ray loud Flat Spectrum Radio Quasars (FSRQs) is evaluated as a function of the location of the γ -ray emission region. This is done self-consistently with parameters inferred from the shape of the spectral energy distribution (SED) in a single-zone leptonic EC-BLR model scenario. We take into account all geometrical effects both in the calculation of the γγ opacity and the normalization of the BLR radiation energy density. As specific examples, we study the FSRQs 3C279 and PKS 1510-089, keeping the BLR radiation energy density at the location of the emission region fixed at the values inferred from the SED. We confirm previous findings that the optical depth due to γγ absorption in the BLR radiation field exceeds unity for both 3C279 and PKS 1510-089 for locations of the γ -ray emission region inside the inner boundary of the BLR. It decreases monotonically, with distance from the central engine and drops below unity for locations within the BLR. For locations outside the BLR, the BLR radiation energy density required for the production of GeV γ -rays rapidly increases beyond observational constraints, thus making the EC-BLR mechanism implausible. Therefore, in order to avoid significant γγ absorption by the BLR radiation field, the γ -ray emission region must therefore be located near the outer boundary of the BLR.

  12. Shelf life determinants and enzyme activities of pearl millet: a comparison of changes in stored flour of hybrids, CMS lines, inbreds and composites.

    Science.gov (United States)

    Goyal, Preeti; Chugh, L K

    2017-09-01

    Shelf life of pearl millet flour is very short because of rapid development of rancidity. This investigation was carried out in view of generating breeding material for development of low rancid pearl millet hybrids/varieties. Flour of twenty-one genotypes; seven hybrids, seven CMS lines, five inbreds and two composites stored in covered aluminium boxes at 37 °C for 30 days along with respective fresh flour was analysed for shelf life indicators/determinants. Crude fat content and fat acidity (FA) of fresh flour of the genotypes varied from 3.8 to 7.2% and 11 to 75 mg KOH/100 g d.m., respectively. FA in stored flour ranged between 180 and 330 mg KOH/100 g d.m. After storage, magnitude of decrease in pH of water extract of flour of the genotypes varied from 0.15 to 0.44. Activity of peroxidase (POX) varied from 378 to 588 units in control flour and irrespective of the genotypes decreased upon storage. Increase in FA (difference between FA of fresh and stored flour) rather total build up of FA was positively associated with crude fat content (r = 0.440*) indicated comparatively more prominent role of lipolytic enzymes. Chemical changes taking place in water soluble fraction of flour were independent of fat content as no correlation was discerned between fat content and decrease in pH. Among the hybrids, HHB 197 had lowest crude fat content (4.7%), lowest total build up FA (212 mg KOH/100 g d.m.), slowest increase in FA (191 mg KOH/100 g d.m.), least decrease in pH (0.31) of water soluble fraction flour during storage and lowest activity of POX in fresh flour (377 units/g d.m). Among all the tested CMS lines, inbreds and composites, HBL 11 showed pattern of quantitative changes in FA, pH and POX activity similar to the hybrid HHB 197 and was identified a promising inbred for developing low-rancid pearl millet variety or hybrid.

  13. Radiation Detection Center on the Front Lines

    International Nuclear Information System (INIS)

    Hazi, A

    2005-01-01

    Many of today's radiation detection tools were developed in the 1960s. For years, the Laboratory's expertise in radiation detection resided mostly within its nuclear test program. When nuclear testing was halted in the 1990s, many of Livermore's radiation detection experts were dispersed to other parts of the Laboratory, including the directorates of Chemistry and Materials Science (CMS); Physics and Advanced Technologies (PAT); Defense and Nuclear Technologies (DNT); and Nonproliferation, Arms Control, and International Security (NAI). The RDC-- was formed to maximize the benefit of radiation detection technologies being developed in 15 to 20 research and development (R and D) programs. These efforts involve more than 200 Laboratory employees across eight directorates, in areas that range from electronics to computer simulations. The RDC's primary focus is the detection, identification, and analysis of nuclear materials and weapons. A newly formed outreach program within the RDC-- is responsible for conducting radiation detection workshops and seminars across the country and for coordinating university student internships. Simon Labov, director of the RDC, says, ''Virtually all of the Laboratory's programs use radiation detection devices in some way. For example, DNT uses radiation detection to create radiographs for their work in stockpile stewardship and in diagnosing explosives; CMS uses it to develop technology for advancing the detection, diagnosis, and treatment of cancer; and the Energy and Environment Directorate uses radiation detection in the Marshall Islands to monitor the aftermath of nuclear testing in the Pacific. In the future, the National Ignition Facility will use radiation detection to probe laser targets and study shock dynamics.''

  14. Characteristics of replication and radiation response of Aedes Albopictus cell line in vitro

    International Nuclear Information System (INIS)

    Lee, C.K.

    1974-01-01

    The radiosensitivity of the line of Aedes albopictus cells was investigated by scoring x-ray-induced chromosome aberrations as a function of dose and of time after irradiation as well as the modification by dose fractionation. In order to obtain these data, a series of studies, e.g., karyotype, cell life cycle, radiation- induced mitotic delay, and frequency and type of spontaneous aberrations, were carried out. Cells from this line had three pairs of chromosomes as a stem line chromosome number. The morphology of the chromosomes is metacentric. Somatic pairing between homologous chromosomes was observed as a common event and there was a high frequency of achromatic gaps on the chromosomes. These observations are in good agreement with those made in other laboratories. The generation time of A. albopictus cell line was approximately 32 hours, in which G 1 , S, and G 2 phases were 2 1 / 2 , 24 and 5 1 / 2 hours respectively. This generation time is in agreement with the population doubling time observed in our cell growth studies. By contrast, in most mammalian cells, G 1 usually is the longest phase and S is comparatively short. Dose fractionation studies indicated that A. albopictus cells in culture may have a faster chromosome repair system than mammalian and other cell systems. This may, at least in part; explain the difference in radiosensitivity between A. albopictus cell line and other cell systems. (Diss. Abstr.)

  15. Measurement of radiation induced transients in hybrid microcircuits by magnetic thin film sensor/recorders

    International Nuclear Information System (INIS)

    Hsieh, E.J.; Vindelov, K.E.; Brown, T.G.; Miller, D.E.

    1976-01-01

    Magnetic thin film transient current sensor/recorders were modified to make two types of nuclear test measurements, transient currents in hybrid microcircuits and internal electromagnetic pulse (IEMP) fields. The measurements were made possible by the invention of split-domain sensor/recorders which can measure bilateral currents and can be reset and readout on location. The sensor/recorders were used in two underground nuclear tests and numerous calibration tests in radiation-simulation machines. The data showed that the nuclear environment had negligible effect on the sensor/recorder's operation and the recorded informations on the sensor/recorders were the signals intended to be monitored. Also, the experimental data agreed with the theoretical analysis in controlled experiments. The data were examined first by on location readout with a magnetic tape viewer and later by Kerr magneto-optic readout in the laboratory. To translate the data into current readings, we reconstructed facsimile data (on each of the sensor/recorders) in the laboratory by current pulses with the same pulse width as the radiation event. An additional check on the accuracy of the data was made by using both the sensor/recorder and the conventional pickup-oscilloscope-camera technique to monitor the same current lead in a simulated radiation environment. Over five runs were made, and the agreement among the two measurement methods was within 25%. The data collectively implied that the measurements were reliable and dependable

  16. Systematic selection for increased fruit yield in populations derived from hybridization only, F1 irradiation, and hybridization following parental irradiation in peanuts (Arachis hypogaea L.)

    International Nuclear Information System (INIS)

    Emery, D.A.; Wynne, J.C.

    1976-01-01

    Three hybrid peanut populations involving a single pair of high yielding parents were developed to determine the effects of irradiation prior to and after hybridization on the response to selection for fruit yield. The control-hybrid population was produced by making reciprocal crosses between the two parents. The pre-hybrid-irradiated population was initiated by making reciprocal crosses between the M 1 plants of the two parents irradiated as seeds. The post-hybrid-irradiated population was developed by irradiating the mature F 1 embryos of crosses between the same parents. Each of the three original populations consisted of 55 F 1 plants. Ten F 2 plants were grown from each F 1 and one F 3 plant from each F 2 was used to initiate the yield tests. Selection for increased yield was practiced systematically and uniformly in each population over the F 3 to F 5 generations until the number of lines derived from single F 1 plants was reduced to five and the number of sublines descended from particular F 2 plants to three per line for yield trials in the F 6 generation. The mean yields of the F 1 derived lines of the irradiated populations were considerably below that of the control hybrid population when selection began but they reached 99% of the control mean in the F 6 generation. Selection gains in the irradiated populations appeared to result from the removal of inferior yielding sublines since greatest progress was made by raising the lower extremities of mean F 2 derived subline ranges rather than by extending the upper extremities of the ranges. The three highest yielding lines in the F6 generation occurred in the irradiated populations while the three highest yielding sublines were found in the hybrid-control population. No incidental association between size and yield of fruit was noted and a wide range of fruit sizes was found among the high yielding lines and sublines in all populations. (author)

  17. Design and initial operation of a two-color soft x-ray camera system on the Compact Toroidal Hybrid experiment

    International Nuclear Information System (INIS)

    Herfindal, J. L.; Dawson, J. D.; Ennis, D. A.; Hartwell, G. J.; Loch, S. D.; Maurer, D. A.

    2014-01-01

    A multi-camera soft x-ray diagnostic has been developed to measure the equilibrium electron temperature profile and temperature fluctuations due to magnetohydrodynamic activity on the Compact Toroidal Hybrid experiment. The diagnostic consists of three separate cameras each employing two 20-channel diode arrays that view the same plasma region through different beryllium filter thicknesses of 1.8 μm and 3.0 μm allowing electron temperature measurements between 50 eV and 200 eV. The Compact Toroidal Hybrid is a five-field period current-carrying stellarator, in which the presence of plasma current strongly modifies the rotational transform and degree of asymmetry of the equilibrium. Details of the soft x-ray emission, effects of plasma asymmetry, and impurity line radiation on the design and measurement of the two-color diagnostic are discussed. Preliminary estimates of the temperature perturbation due to sawtooth oscillations observed in these hybrid discharges are given

  18. Actinide L-line ED-XRF and Hybrid K-edge Densitometer Spectra Processing

    International Nuclear Information System (INIS)

    Esbelin, E.

    2015-01-01

    The analysis laboratory in the CEA Atalante complex at Marcoule (France) performs numerous R and D studies carried out in glove-boxes or in hot cells. Most of the samples are measured in liquid phase, aqueous or organic. The concentration of the main actinides of interest (U, Np, Pu, Am and Cm) are determined by XRF in a hot cell via their L-line X-ray between 13 and 15 keV. In order to limit the counting rate of many radioactive emitters (X-ray and gamma emitters) in the analysis solution and the continuous spectrum, a graphite monochromator is placed between the sample and detector. Commercial or free, the software packages available for processing X-ray spectra are designed and dedicated to a specific instrument and/or do not take into account the specific feature of our system, in other words, the presence of a monochromator. Therefore, a new X-ray analysis software programme was developed for this particular system which takes into account matrix effects corrections. For sample with U and/or Pu in high concentrations, the hybrid K-edge densitometer is used. A new software programme was also developed. For K-edge densitometry spectra processing, no calibration process is used. Spectra processing is based on theoretical equation and uses XCOM database for mass attenuation coefficients. Measured spectra on K-edge densitometer of Rokkasho Safeguards Analytical Laboratory were processed with this software and a very good agreement was found with IDTIMS results. The new graphical user interface allows to manually correct the defined edge. For the XRF spectra processing, new algorithms are used to define the base line and to find/integrate peaks. With these two analytical devices in laboratory, U and Pu concentrations can be measured from 0.5 mg/l to several hundred of g/l. (author)

  19. LHCb: Radiation hard programmable delay line for LHCb Calorimeter Upgrade

    CERN Multimedia

    Mauricio Ferre, J; Vilasís Cardona, X; Picatoste Olloqui, E; Machefert, F; Lefrançois, J; Duarte, O

    2013-01-01

    This poster describes the implementation of a SPI-programmable clock delay chip based on a Delay Locked Loop (DLL) in order to shift the phase of the LHC clock (25 ns) in steps of 1ns, with a 4ps jitter and 18ps of DNL. The delay lines will be integrated into ICECAL, the LHCb calorimeter front-end ASIC in the near future. The stringent noise requirements on the ASIC imply minimizing the noise contribution of digital components. This is accomplished by implementing the DLL in differential mode. To achieve the required radiation tolerance several techniques are applied: double guard rings between PMOS and NMOS transistors as well as glitch suppressors and TMR Registers. This 5.7 mm2 chip has been implemented in CMOS 0.35um technology.

  20. 7 CFR 201.78 - Pollen control for hybrids.

    Science.gov (United States)

    2010-01-01

    ... line used to pollinate an A Line and to restore fertility in the resulting hybrid seed.) (b) Corn. When 5 percent or more of the seed parent plants have receptive silks, shedding tassels in the seed...

  1. Genome Dynamics of Hybrid Saccharomyces cerevisiae During Vegetative and Meiotic Divisions

    Directory of Open Access Journals (Sweden)

    Abhishek Dutta

    2017-11-01

    Full Text Available Mutation and recombination are the major sources of genetic diversity in all organisms. In the baker’s yeast, all mutation rate estimates are in homozygous background. We determined the extent of genetic change through mutation and loss of heterozygosity (LOH in a heterozygous Saccharomyces cerevisiae genome during successive vegetative and meiotic divisions. We measured genome-wide LOH and base mutation rates during vegetative and meiotic divisions in a hybrid (S288c/YJM789 S. cerevisiae strain. The S288c/YJM789 hybrid showed nearly complete reduction in heterozygosity within 31 generations of meioses and improved spore viability. LOH in the meiotic lines was driven primarily by the mating of spores within the tetrad. The S288c/YJM789 hybrid lines propagated vegetatively for the same duration as the meiotic lines, showed variable LOH (from 2 to 3% and up to 35%. Two of the vegetative lines with extensive LOH showed frequent and large internal LOH tracts that suggest a high frequency of recombination repair. These results suggest significant LOH can occur in the S288c/YJM789 hybrid during vegetative propagation presumably due to return to growth events. The average base substitution rates for the vegetative lines (1.82 × 10−10 per base per division and the meiotic lines (1.22 × 10−10 per base per division are the first genome-wide mutation rate estimates for a hybrid yeast. This study therefore provides a novel context for the analysis of mutation rates (especially in the context of detecting LOH during vegetative divisions, compared to previous mutation accumulation studies in yeast that used homozygous backgrounds.

  2. A gene-based radiation hybrid map of the gilthead sea bream Sparus aurata refines and exploits conserved synteny with Tetraodon nigroviridis

    Directory of Open Access Journals (Sweden)

    Tsalavouta Matina

    2007-02-01

    Full Text Available Abstract Background Comparative teleost studies are of great interest since they are important in aquaculture and in evolutionary issues. Comparing genomes of fully sequenced model fish species with those of farmed fish species through comparative mapping offers shortcuts for quantitative trait loci (QTL detections and for studying genome evolution through the identification of regions of conserved synteny in teleosts. Here a comparative mapping study is presented by radiation hybrid (RH mapping genes of the gilthead sea bream Sparus aurata, a non-model teleost fish of commercial and evolutionary interest, as it represents the worldwide distributed species-rich family of Sparidae. Results An additional 74 microsatellite markers and 428 gene-based markers appropriate for comparative mapping studies were mapped on the existing RH map of Sparus aurata. The anchoring of the RH map to the genetic linkage map resulted in 24 groups matching the karyotype of Sparus aurata. Homologous sequences to Tetraodon were identified for 301 of the gene-based markers positioned on the RH map of Sparus aurata. Comparison between Sparus aurata RH groups and Tetraodon chromosomes (karyotype of Tetraodon consists of 21 chromosomes in this study reveals an unambiguous one-to-one relationship suggesting that three Tetraodon chromosomes correspond to six Sparus aurata radiation hybrid groups. The exploitation of this conserved synteny relationship is furthermore demonstrated by in silico mapping of gilthead sea bream expressed sequence tags (EST that give a significant similarity hit to Tetraodon. Conclusion The addition of primarily gene-based markers increased substantially the density of the existing RH map and facilitated comparative analysis. The anchoring of this gene-based radiation hybrid map to the genome maps of model species broadened the pool of candidate genes that mainly control growth, disease resistance, sex determination and reversal, reproduction as well

  3. Preliminary Study on Hybrid Computational Phantom for Radiation Dosimetry Based on Subdivision Surface

    International Nuclear Information System (INIS)

    Jeong, Jong Hwi; Choi, Sang Hyoun; Cho, Sung Koo; Kim, Chan Hyeong

    2007-01-01

    The anthropomorphic computational phantoms are classified into two groups. One group is the stylized phantoms, or MIRD phantoms, which are based on mathematical representations of the anatomical structures. The shapes and positions of the organs and tissues in these phantoms can be adjusted by changing the coefficients of the equations in use. The other group is the voxel phantoms, which are based on tomographic images of a real person such as CT, MR and serially sectioned color slice images from a cadaver. Obviously, the voxel phantoms represent the anatomical structures of a human body much more realistically than the stylized phantoms. A realistic representation of anatomical structure is very important for an accurate calculation of radiation dose in the human body. Consequently, the ICRP recently has decided to use the voxel phantoms for the forthcoming update of the dose conversion coefficients. However, the voxel phantoms also have some limitations: (1) The topology and dimensions of the organs and tissues in a voxel model are extremely difficult to change, and (2) The thin organs, such as oral mucosa and skin, cannot be realistically modeled unless the voxel resolution is prohibitively high. Recently, a new approach has been implemented by several investigators. The investigators converted their voxel phantoms to hybrid computational phantoms based on NURBS (Non-Uniform Rational B-Splines) surface, which is smooth and deformable. It is claimed that these new phantoms have the flexibility of the stylized phantom along with the realistic representations of the anatomical structures. The topology and dimensions of the anatomical structures can be easily changed as necessary. Thin organs can be modeled without affecting computational speed or memory requirement. The hybrid phantoms can be also used for 4-D Monte Carlo simulations. In this preliminary study, the external shape of a voxel phantom (i.e., skin), HDRK-Man, was converted to a hybrid computational

  4. Marker-Assisted Backcrossing to Develop an Elite Cytoplasmic Male Sterility Line in Rice

    Directory of Open Access Journals (Sweden)

    Asadollah Ahmadikhah

    2015-07-01

    Full Text Available Cytoplasmic male sterility (CMS is a cornerstone of hybrid production in many crops. In three-line hybrid systems, use of CMS, maintainer, and fertility restorer lines is necessary for production of hybrid seeds. Limited resources of CMS and low variation of CMS lines cause genetic vulnerability to pathogens. Therefore, diversifying the CMS sources is indispensible for a sustainable production system of hybrid seed. In this study, we attempted for the first time to transfer CMS into maintainer line Yosen B in restricted generations using the marker-assisted backcrossing (MABC method. The resultant F hybrid of IR68897 A/Yosen B cross was backcrossed to Yosen B, and CMS plants in each backcross generation (from BCF to BCF were selected based on phenotyping test and MABC. Molecular assessment of backcross progenies was conducted using a mitochondrial CMS-specific marker and 34 polymorphic nuclear simple-sequence repeat (SSR markers in early generations (from BCF to BCF and was continued using 9 additional SSRs and 82 inter-simple sequence repeat (ISSR markers in BCF. A MABC strategy could successfully recover the recurrent parent genome (RPG in BCF generation, and decreased heterozygosity of final CMS plants. Restorability test with known wild-abortive restorer lines (viz. IR36 and IR24 showed that combination of Yosen A × IR24 could produce highly fertile F hybrid. Evaluation of some important agronomic traits of the final CMS line (BCF at field condition showed that it was comparable to the original maintainer fertile counterpart. Phenotypic and marker-assisted selections could considerably decrease the time needed for full recovery of RPG so that final CMS line could show a high similarity to original fertile counterpart.

  5. Enhancement of radiation cytotoxicity by gold nanoparticles in MCF-7 breast cancer cell lines

    Energy Technology Data Exchange (ETDEWEB)

    Rosli, Nur Shafawati binti; Rahman, Azhar Abdul [School of Physics, Universiti Sains Malaysia, 11800, Pulau Pinang (Malaysia); Aziz, Azlan Abdul [School of Physics, Universiti Sains Malaysia, 11800, Pulau Pinang (Malaysia); Nano-Biotechnology Research and Innovation (NanoBRI), Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, 11800, Pulau Pinang (Malaysia); Shamsuddin, Shaharum [Nano-Biotechnology Research and Innovation (NanoBRI), Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, 11800, Pulau Pinang (Malaysia); School of Health Sciences, Health Campus, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan (Malaysia)

    2015-04-24

    Therapy combined with metallic nanoparticles is a new way to treat cancer, in which gold nanoparticles (AuNPs) are injected through intravenous administration and bound to tumor sites. Radiotherapy aims to deliver a high therapeutic dose of ionizing radiation to the tumor without exceeding normal tissue tolerance. The use of AuNPs which is a high-atomic-number (Z) material in radiotherapy will provide a high probability for photon interaction by photoelectric effect. These provide advantages in terms of radiation dose enhancement. The high linear energy transfer and short range of photoelectric interaction products (photoelectrons, characteristic x-rays, Auger electrons) produce localized dose enhancement of the tumor. In this work, breast cancer cell lines (MCF-7) are seeded in the 96-well plate and were treated with 13 nm AuNPs before they were irradiated with 6 MV and 10 MV photon beam from a medical linear accelerator at various radiation doses. To validate the enhanced killing effect, both with and without AuNPs MCF-7 cells is irradiated simultaneously. By comparison, the results show that AuNPs significantly enhance cancer killing.

  6. Radiosensitivity of mesothelioma cell lines

    International Nuclear Information System (INIS)

    Haekkinen, A.M.; Laasonen, A.; Linnainmaa, K.; Mattson, K.; Pyrhoenen, S.

    1996-01-01

    The present study was carried out in order to examine the radiosensitivity of malignant pleural mesothelioma cell lines. Cell kinetics, radiation-induced delay of the cell cycle and DNA ploidy of the cell lines were also determined. For comparison an HeLa and a human foetal fibroblast cell line were simultaneously explored. Six previously cytogenetically and histologically characterized mesothelioma tumor cell lines were applied. A rapid tiazolyl blue microtiter (MTT) assay was used to analyze radiosensitivity and cell kinetics and DNA ploidy of the cultured cells were determined by flow cytometry. The survival fraction after a dose of 2 Gy (SF2), parameters α and β of the linear quadratic model (LQ-model) and mean inactivation dose (D MID ) were also estimated. The DNA index of four cell lines equaled 1.0 and two cell lines equaled 1.5 and 1.6. Different mesothelioma cell lines showed a great variation in radiosensitivity. Mean survival fraction after a radiation dose of 2 Gy (SF2) was 0.60 and ranged from 0.36 to 0.81 and mean α value was 0.26 (range 0.48-0.083). The SF2 of the most sensitive diploid mesothelioma cell line was 0.36: Less than that of the foetal fibroblast cell line (0.49). The survival fractions (0.81 and 0.74) of the two most resistant cell lines, which also were aneuploid, were equal to that of the HeLa cell line (0.78). The α/β ratios of the most sensitive cell lines were almost an order of magnitude greater than those of the two most resistant cell lines. Radiation-induced delay of the most resistant aneuploid cell line was similar to that of HeLa cells but in the most sensitive (diploid cells) there was practically no entry into the G1 phase following the 2 Gy radiation dose during 36 h. (orig.)

  7. Radiosensitivity of mesothelioma cell lines

    Energy Technology Data Exchange (ETDEWEB)

    Haekkinen, A.M. [Dept. of Oncology, Univ. Central Hospital, Helsinki (Finland); Laasonen, A. [Dept. of Pathology, Central Hospital of Etelae-Pohjanmaa, Seinaejoki (Finland); Linnainmaa, K. [Dept. of Industrial Hygiene and Toxicology, Inst. of Occupational Health, Helsinki (Finland); Mattson, K. [Dept. Pulmonary Medicine, Univ. Central Hospital, Helsinki (Finland); Pyrhoenen, S. [Dept. of Oncology, Univ. Central Hospital, Helsinki (Finland)

    1996-10-01

    The present study was carried out in order to examine the radiosensitivity of malignant pleural mesothelioma cell lines. Cell kinetics, radiation-induced delay of the cell cycle and DNA ploidy of the cell lines were also determined. For comparison an HeLa and a human foetal fibroblast cell line were simultaneously explored. Six previously cytogenetically and histologically characterized mesothelioma tumor cell lines were applied. A rapid tiazolyl blue microtiter (MTT) assay was used to analyze radiosensitivity and cell kinetics and DNA ploidy of the cultured cells were determined by flow cytometry. The survival fraction after a dose of 2 Gy (SF2), parameters {alpha} and {beta} of the linear quadratic model (LQ-model) and mean inactivation dose (D{sub MID}) were also estimated. The DNA index of four cell lines equaled 1.0 and two cell lines equaled 1.5 and 1.6. Different mesothelioma cell lines showed a great variation in radiosensitivity. Mean survival fraction after a radiation dose of 2 Gy (SF2) was 0.60 and ranged from 0.36 to 0.81 and mean {alpha} value was 0.26 (range 0.48-0.083). The SF2 of the most sensitive diploid mesothelioma cell line was 0.36: Less than that of the foetal fibroblast cell line (0.49). The survival fractions (0.81 and 0.74) of the two most resistant cell lines, which also were aneuploid, were equal to that of the HeLa cell line (0.78). The {alpha}/{beta} ratios of the most sensitive cell lines were almost an order of magnitude greater than those of the two most resistant cell lines. Radiation-induced delay of the most resistant aneuploid cell line was similar to that of HeLa cells but in the most sensitive (diploid cells) there was practically no entry into the G1 phase following the 2 Gy radiation dose during 36 h. (orig.).

  8. Radiation up-regulated the expression of VEGF in a canine oral melanoma cell line

    International Nuclear Information System (INIS)

    Flickinger, I.; Rütgen, B.C.; Gerner, W.; Tichy, A.; Saalmüller, A.; Kleiter, M.; Calice, I.

    2013-01-01

    To evaluate radiosensitivity and the effects of radiation on the expression of vascular endothelial growth factor (VEGF) and VEGF receptors in the canine oral melanoma cell line, TLM 1, cells were irradiated with doses of 0, 2, 4, 6, 8 and 10 Gray (Gy). Survival rates were then determined by a MTT assay, while vascular endothelial growth factor receptor (VEGFR)-1 and -2 expression was measured by flow cytometry and apoptotic cell death rates were investigated using an Annexin assay. Additionally, a commercially available canine VEGF ELISA kit was used to measure VEGF. Radiosensitivity was detected in TLM 1 cells, and mitotic and apoptotic cell death was found to occur in a radiation dose dependent manner. VEGF was secreted constitutively and significant up-regulation was observed in the 8 and 10 Gy irradiated cells. In addition, a minor portion of TLM 1 cells expressed vascular endothelial growth factor receptor (VEGFR)-1 intracellularly. VEGFR-2 was detected in the cytoplasm and was down-regulated following radiation with increasing dosages. In TLM 1 cells, apoptosis plays an important role in radiation induced cell death. It has also been suggested that the significantly higher VEGF production in the 8 and 10 Gy group could lead to tumour resistance. (author)

  9. TERRA Expression Levels Do Not Correlate With Telomere Length and Radiation Sensitivity in Human Cancer Cell Lines

    Directory of Open Access Journals (Sweden)

    Alexandra eSmirnova

    2013-05-01

    Full Text Available Mammalian telomeres are transcribed into long non-coding telomeric RNA molecules (TERRA that seem to play a role in the maintenance of telomere stability. In human cells, CpG island promoters drive TERRA transcription and are regulated by methylation. It was suggested that the amount of TERRA may be related to telomere length. To test this hypothesis we measured telomere length and TERRA levels in single clones isolated from five human cell lines: HeLa (cervical carcinoma, BRC-230 (breast cancer, AKG and GK2 (gastric cancers and GM847 (SV40 immortalized skin fibroblasts. We observed great clonal heterogeneity both in TRF (Terminal Restriction Fragment length and in TERRA levels. However, these two parameters did not correlate with each other. Moreover, cell survival to γ-rays did not show a significant variation among the clones, suggesting that, in this cellular system, the intra-population variability in telomere length and TERRA levels does not influence sensitivity to ionizing radiation. This conclusion was supported by the observation that in a cell line in which telomeres were greatly elongated by the ectopic expression of telomerase, TERRA expression levels and radiation sensitivity were similar to the parental HeLa cell line.

  10. Regeneration of somatic hybrids in relation to the nuclear and cytoplasmic genomes of wheat and Setaria italica.

    Science.gov (United States)

    Xiang, Fengning; Xia, Guangmin; Zhi, Daying; Wang, Jing; Nie, Hui; Chen, Huimin

    2004-08-01

    Somatic hybridization via PEG (Polyethylene 6000)-mediated protoplast fusion was achieved between two different wheat culture lines (Triticum aestivum L., "Jinan"177, T1 and T2) and Setaria italica (L.) P. Beauv. The T1 recipient originated from non-regenerable long-term cell suspensions, while T2 was derived from embryogenic calli with a high regeneration capacity. Donor protoplasts were obtained from embryogenic calli of S. italica (S) (with low regeneration capacity) irradiated with different doses of ultraviolet light. Twenty-three putative hybrid cell lines were produced in fusion combinations with the donor protoplasts treated with UV light for 30 s (combination I) and 1 min (combination II), but only one (from combination II) differentiated into green plants. Three cell lines from combination I and five cell lines from combination II possessed the nuclear genomes of T1, T2, and S. italica as revealed by cytological, isozyme, RAPD, and 5S rDNA spacer sequence analyses. Genomic in situ hybridization (GISH) analysis showed that most hybrid cell lines had 22-36 wheat chromosomes, 0-2 S. italica chromosomes, and 1-6 wheat - S. italica recombinant chromosomes, whereas the regenerable cell line had 44-56 wheat chromosomes and 3-6 recombinant chromosomes, but no intact S. italica chromosomes. RFLP analysis of organellar DNA revealed that mitochondrial and chloroplast DNA of both parents coexisted in all hybrid cell lines and recombined in most hybrid cell lines. These results indicate that the regeneration of hybrid plants involves not only the integration of S. italica nuclear and organellar DNA, but also the genome complementation of T1 and T2.

  11. Suppression or knockout of SaF/SaM overcomes the Sa-mediated hybrid male sterility in rice

    Institute of Scientific and Technical Information of China (English)

    Yongyao Xie; Baixiao Niu; Yunming Long; Gousi Li; Jintao Tang; Yaling Zhang; Ding Ren; Yao-Guang Liu; Letian Chen

    2017-01-01

    Hybrids between the indica and japonica subspecies of rice (Oryza sativa) are usually sterile, which hinders utilization of heterosis in the inter-subspecific hybrid breeding. The complex locus Sa comprises two adjacently located genes, SaF and SaM, which interact to cause abortion of pollen grains carrying the japonica allele in japonica-indica hybrids. Here we showed that silencing of SaF or SaM by RNA interference restored male fertility in indica-japonica hybrids with heterozygous Sa. We further used clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9-based genome editing to knockout the SaF and SaM alleles, respectively, of an indica rice line to create hybrid-compatible lines. The resultant artificial neutral alleles did not affect pollen viability and other agricultural traits, but did break down the reproductive barrier in the hybrids. We found that some rice lines have natural neutral allele Sa-n, which was compatible with the typical japonica or indica Sa alleles in hybrids. Our results demonstrate that SaF and SaM are required for hybrid male sterility, but are not essential for pollen development. This study provides effective approaches for the generation of hybrid-compatible lines by knocking out the Sa locus or using the natural Sa-n allele to overcome hybrid male sterility in rice breeding.

  12. Heterosis and correlation in interspecific and intraspecific hybrids of cotton.

    Science.gov (United States)

    Munir, S; Hussain, S B; Manzoor, H; Quereshi, M K; Zubair, M; Nouman, W; Shehzad, A N; Rasul, S; Manzoor, S A

    2016-06-24

    Interspecific and intraspecific hybrids show varying degrees of heterosis for yield and yield components. Yield-component traits have complex genetic relationships with each other. To determine the relationship of yield-component traits and fiber traits with seed cotton yield, six lines (Bt. CIM-599, CIM-573, MNH-786, CIM-554, BH-167, and GIZA-7) and three test lines (MNH-886, V4, and CIM-557) were crossed in a line x tester mating design. Heterosis was observed for seed cotton yield, fiber traits, and for other yield-component traits. Heterosis in interspecific hybrids for seed cotton yield was more prominent than in intraspecific hybrids. The interspecific hybrid Giza-7 x MNH-886 had the highest heterosis (114.77), while among intraspecific hybrids, CIM-554 x CIM-557 had the highest heterosis (61.29) for seed cotton yield. A major trait contributing to seed cotton yield was bolls/plant followed by boll weight. Correlation studies revealed that bolls/plant, boll weight, lint weight/boll, lint index, seed index, lint/seed, staple length, and staple strength were significantly and positively associated with seed cotton yield. Selection based on boll weight, boll number, lint weight/boll, and lint index will be helpful for improving cotton seed yield.

  13. On-line energy and battery thermal management for hybrid electric heavy-duty truck

    NARCIS (Netherlands)

    Pham, H.T.; Kessels, J.T.B.A.; Bosch, van den P.P.J.; Huisman, R.G.M.; Nevels, R.M.P.A.

    2013-01-01

    This paper discusses an integrated approach for energy and thermal management to minimize the fuel consumption of a hybrid electric heavy-duty truck. Conventional Energy Management Systems (EMS) operate separately from the Battery Thermal Management System (BTMS) in Hybrid Electric Vehicles (HEVs).

  14. Ultra high molecular weight polyethylene (UHMWPE) fiber epoxy composite hybridized with Gadolinium and Boron nanoparticles for radiation shielding

    Science.gov (United States)

    Mani, Venkat; Prasad, Narasimha S.; Kelkar, Ajit

    2016-09-01

    Deep space radiations pose a major threat to the astronauts and their spacecraft during long duration space exploration missions. The two sources of radiation that are of concern are the galactic cosmic radiation (GCR) and the short lived secondary neutron radiations that are generated as a result of fragmentation that occurs when GCR strikes target nuclei in a spacecraft. Energy loss, during the interaction of GCR and the shielding material, increases with the charge to mass ratio of the shielding material. Hydrogen with no neutron in its nucleus has the highest charge to mass ratio and is the element which is the most effective shield against GCR. Some of the polymers because of their higher hydrogen content also serve as radiation shield materials. Ultra High Molecular Weight Polyethylene (UHMWPE) fibers, apart from possessing radiation shielding properties by the virtue of the high hydrogen content, are known for extraordinary properties. An effective radiation shielding material is the one that will offer protection from GCR and impede the secondary neutron radiations resulting from the fragmentation process. Neutrons, which result from fragmentation, do not respond to the Coulombic interaction that shield against GCR. To prevent the deleterious effects of secondary neutrons, targets such as Gadolinium are required. In this paper, the radiation shielding studies that were carried out on the fabricated sandwich panels by vacuum-assisted resin transfer molding (VARTM) process are presented. VARTM is a manufacturing process used for making large composite structures by infusing resin into base materials formed with woven fabric or fiber using vacuum pressure. Using the VARTM process, the hybridization of Epoxy/UHMWPE composites with Gadolinium nanoparticles, Boron, and Boron carbide nanoparticles in the form of sandwich panels were successfully carried out. The preliminary results from neutron radiation tests show that greater than 99% shielding performance was

  15. A hybrid numerical prediction scheme for solar radiation estimation in un-gauged catchments.

    Science.gov (United States)

    Shamim, M. A.; Bray, M.; Ishak, A. M.; Remesan, R.; Han, D.

    2009-09-01

    The importance of solar radiation on earth's surface is depicted in its wide range of applications in the fields of meteorology, agricultural sciences, engineering, hydrology, crop water requirements, climatic changes and energy assessment. It is quite random in nature as it has to go through different processes of assimilation and dispersion while on its way to earth. Compared to other meteorological parameters, solar radiation is quite infrequently measured, for example, the worldwide ratio of stations collecting solar radiation to those collecting temperature is 1:500 (Badescu, 2008). Researchers, therefore, have to rely on indirect techniques of estimation that include nonlinear models, artificial intelligence (e.g. neural networks), remote sensing and numerical weather predictions (NWP). This study proposes a hybrid numerical prediction scheme for solar radiation estimation in un-gauged catchments. It uses the PSU/NCAR's Mesoscale Modelling system (MM5) (Grell et al., 1995) to parameterise the cloud effect on extraterrestrial radiation by dividing the atmosphere into four layers of very high (6-12 km), high (3-6 km), medium (1.5-3) and low (0-1.5) altitudes from earth. It is believed that various cloud forms exist within each of these layers. An hourly time series of upper air pressure and relative humidity data sets corresponding to all of these layers is determined for the Brue catchment, southwest UK, using MM5. Cloud Index (CI) was then determined using (Yang and Koike, 2002): 1 p?bi [ (Rh - Rh )] ci =------- max 0.0,---------cri dp pbi - ptipti (1- Rhcri) where, pbi and pti represent the air pressure at the top and bottom of each layer and Rhcri is the critical value of relative humidity at which a certain cloud type is formed. Output from a global clear sky solar radiation model (MRM v-5) (Kambezidis and Psiloglu, 2008) is used along with meteorological datasets of temperature and precipitation and astronomical information. The analysis is aided by the

  16. Transcriptome-wide studies of prostate cancer cell lines in the context of medical radiation; Transkriptomweite Untersuchungen von Prostata-Krebszelllinien im Kontext medizinischer Strahlentherapie

    Energy Technology Data Exchange (ETDEWEB)

    Hammer, Paul

    2012-06-26

    The use of radiotherapy in addition to chemotherapy and surgical removal is the most powerful instrument in the fight against malignant tumors in cancer medicine. After cardiovascular diseases, cancer is the second leading cause of death in the western world, in which prostate cancer is the most frequent male cancer. Despite continuous technological improvements in radiological instruments and prognosis, it may occur a recurrence up to many years after radiotherapy due to a high resistance capability of individual malignant cells of the locally occurring tumor. Although modern radiation biology has studied many aspects of the resistance mechanisms, questions are largely unanswered especially in regards to prognostic terms and time response of tumor cells to ionizing radiation. As cellular models four prostate cancer cell lines with different radiation sensitivities (PC3, DuCaP, DU-145, RWPE-1) were cultured and tested for their ability to survive after exposure to ionizing radiation by a trypane blue and MTT viability assay. The proliferative capacity of the four cell lines was determined using a colony formation assay. The PC3 cell line (radiation-resistant) and the DuCaP cell line (radiation-sensitive) showed the maximal differences in terms of radiation sensitivity. Based on these results the two cell lines were selected to allow identification of potential prognostic marker for predicting the effectiveness of radiation therapy via their transcriptome-wide gene expression. Furthermore, a time series experiment with the radiation-resistant PC3 cell line was performed. At 8 different time points, during the period from 00:00 - 42:53 (hh:mm) after exposure with 1 Gy, the mRNA was quantified by next generation sequencing to investigate the dynamic behavior of time-delayed gene expression and to discover resistance mechanisms. Of 10,966 expressed genes 730 were significant differentially expressed, determined by setting a fold change threshold in conjunction with a P

  17. Auroral electron acceleration by lower-hybrid waves

    International Nuclear Information System (INIS)

    Bingham, R.; Bryant, D.A.; Hall, D.S.

    1986-01-01

    Because the particles and electric fields association with inverted-V electron streams do not have the characteristics expected for acceleration by a quasistatic potential difference, the possiblity that the electrons are stochastically accelerated by waves is investigated. It is demonstrated that the lower hybrid waves seen on auroral field lines have the righ properties to account for the electron acceleration. It is further shown that the lower hybrid wave power measured on auroral field lines can be generated by the streaming ions observed at the boundary of the plasma sheet, and that this wave power is sufficient to account for the electron power observed close to the atmosphere. (author)

  18. Deterministic linear-optics quantum computing based on a hybrid approach

    International Nuclear Information System (INIS)

    Lee, Seung-Woo; Jeong, Hyunseok

    2014-01-01

    We suggest a scheme for all-optical quantum computation using hybrid qubits. It enables one to efficiently perform universal linear-optical gate operations in a simple and near-deterministic way using hybrid entanglement as off-line resources

  19. Deterministic linear-optics quantum computing based on a hybrid approach

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Seung-Woo; Jeong, Hyunseok [Center for Macroscopic Quantum Control, Department of Physics and Astronomy, Seoul National University, Seoul, 151-742 (Korea, Republic of)

    2014-12-04

    We suggest a scheme for all-optical quantum computation using hybrid qubits. It enables one to efficiently perform universal linear-optical gate operations in a simple and near-deterministic way using hybrid entanglement as off-line resources.

  20. Enhancement of Temozolomide and radiation induced damage in malignant glioma cell lines by 2-deoxy-D-glucose

    International Nuclear Information System (INIS)

    Kumari, Kalyani; Shyam, Sai; Chandrasekhar Sagar, B.K.; Jagath Lal, G.; Kalia, Vijay Kumar

    2014-01-01

    Malignant Gliomas are the most common and aggressive CNS tumors. The current standard treatment includes surgery, followed by Temozolomide (TMZ)-Radiotherapy. It leads to increased survival as compared to radiotherapy alone. However hematological toxicities are also increased by the combination treatments. Therefore, it is important to carry out further preclinical studies, to develop more effective treatment for these tumors. 2-deoxy-D-Glucose (2-DG), an inhibitor of glycolytic energy metabolism, has been shown earlier to differentially inhibit growth and survival of tumor cells in vitro. It also increases tumor regression in experimental models; and has been used in a few clinical studies as radiosensitizer. In the present study, effects of combining 2-DG with TMZ on radiation induced damage were studied in established malignant glioma cell lines (U251MG and U87MG); and primary cultures derived from malignant glioma biopsies. Exponentially growing cells were exposed to drugs and radiation. Drugs were removed 4 hours later and cultures were processed further for different assays of damage. Effects on proliferation response, viability and total cellular damage (TCD; micronuclei + apoptosis) were studied after post-treatment growth for 1, 2, 4 or 6 days. Our results showed that combination of 2-DG with TMZ ± Radiation significantly inhibited tumor cell proliferation up to 6 days, at low drug concentrations in primary as well as in established cell lines. The TCD at 24 and 48 hours after Gamma irradiation was also significantly increased by the combination of drugs as compared to individual treatments. Experiments to study proliferation kinetics by flow cytometry and cell survival are in progress. These studies suggest that 2-DG significantly enhances the cytotoxic effect of TMZ + radiation without increasing toxic side effects. Therefore, combining 2-DG with TMZ+ radiation therapy could be a potential strategy to improve the therapeutic outcome for Malignant

  1. PARTICULARITIES OF WHITE HEAD CABBAGE F1 HYBRID BREEDING FOR PRIDNESTROVIAN MOLDAVIAN REPUBLIC CONDITIONS

    Directory of Open Access Journals (Sweden)

    L. I. Shpak

    2016-01-01

    Full Text Available A model of late maturing F1 hybrid of white head cabbage for long-term storing and suitable for national recipe “golubci” have been developed based on requirements for varieties and F1 hybrids grown in the South in the conditions of drought, high temperatures and low air humidity. Combining ability of seven self-incompatible inbred lines of white head cabbage was studied by the system of full diallel crosses in conditions of Pridnestrovian Moldavian Republic. These lines were developed on the base of heat tolerant varieties ‘Biruchekutskaya’, ‘Volna’, ‘Lada’, ‘Moldavanka’ and ‘Kharkovskaya Zimnyaya’ bred in the south. Out of 42 hybrid combinations studied 15 significantly surpassed in productivity the variety ‘Zavadovskaya’ that is traditionally grown by local farmers for pickling and recipe “golubci”. High GCA effects for productivity showed the following breeding lines: Bu1, Ml3 and Kl5. Polygenes, controlling the high yield ability, mainly were dominant and single-directed, however the correlation betweenyield ability and GCA effect was middle, r=0.63±0.35. No correlation was observed inbreeding line between GCA effect and truly heterosis effect in hybrid combinations (r=0.19. Disease resistance analysis revealed lines Bu1 and Kl5 that had shown resistance to Fusarium yellows. Two hybrid combination Kl5xBu1 and Ml3xBu1 were highly tolerant to thrips attacks according to analysis of all promising hybrid combinations, carried out in natural infection background. Weak correlation was observed between the number of damaged leaves in cabbage head and dry matter content, r=0.41±0.21, and also there is no correlation revealed with sugar contents, ascorbic acid and nitrate content. High tolerance of hybrids with participation of line Bu1 explained the partial dominance nature of the trait. Hybrid combinations Kl5xBu1 and Ml3xBu1 called ‘Batal’ and ‘Shedevr’, respectively, included in registry of Moldova and

  2. Radiation-induced apoptosis and cell cycle checkpoints in human colorectal tumour cell lines

    International Nuclear Information System (INIS)

    Playle, L.C.

    2001-03-01

    The p53 tumour suppressor gene is mutated in 75% of colorectal carcinomas and is critical for DNA damage-induced G1 cell cycle arrest. Data presented in this thesis demonstrate that after treatment with Ionizing Radiation (IR), colorectal tumour cell lines with mutant p53 are unable to arrest at G1 and undergo cell cycle arrest at G2. The staurosporine derivative, UCN-01, was shown to abrogate the IR-induced G2 checkpoint in colorectal tumour cell lines. Furthermore, in some cell lines, abrogation of the G2 checkpoint was associated with radiosensitisation. Data presented in this study demonstrate that 2 out of 5 cell lines with mutant p53 were sensitised to IR by UCN-01. In order to determine whether radiosensitisation correlated with lack of functional p53, transfected derivatives of an adenoma-derived cell line were studied, in which endogenous wild type p53 was disrupted by expression of a dominant negative p53 mutant protein (and with a vector control). In both these cell lines UCN-01 abrogated the G2 arrest however this was not associated with radiosensitisation, indicating that radiosensitisation is a cell type-specific phenomenon. Although 2 colorectal carcinoma cell lines, with mutant p53, were sensitised to IR by UCN-01, the mechanisms of p53-independent IR-induced apoptosis in the colon are essentially unknown. The mitogen-activated protein kinase (MAPK) pathways (that is the JNK, p38 and ERK pathways) have been implicated in apoptosis in a range of cell systems and in IR-induced apoptosis in some cell types. Data presented in this study show that, although the MAPKs can be activated by the known activator anisomycin, there is no evidence of a role for MAPKs in IR-induced apoptosis in colorectal tumour cell lines, regardless of p53 status. In summary, some colorectal tumour cell lines with mutant p53 can be sensitised to IR-induced cell death by G2 checkpoint abrogation and this may be an important treatment strategy, however mechanisms of IR-induced p53

  3. New hybrid rice cultivar 'Zhefuliangyou 12' with improved grain quality produced by leaf color marker-labeled male sterile line and mutant with enhanced tillers and improved grain quality

    International Nuclear Information System (INIS)

    Mei Shufang; Zhao Hua; Wang Yongqiang; Shu Xiaoli; Wu Dianxing

    2013-01-01

    In order to breed high yielding and good grain quality new variety with controllable seed purity, hybrid rice 'Zhefuliangyou 12' was produced by green-revertible albino leaf color marker-labeled two-line male sterile line 'NHR111S' and mutant 'ZF-2' with enhanced tillers and improved grain quality, which was characterized by improved grain quality, rice blast resistance and lodging resistance. Breeding protocol, characteristics, and high yielding cultivation techniques of 'Zhefuliangyou 12' were briefly introduced in the current paper. (authors)

  4. The anti-epidermal growth factor receptor (EGFR) monoclonal antibody, C225, enhances radiation-induced apoptosis in primary glioma cell lines through mediation of MAPK/JNK/p38 signaling pathways

    International Nuclear Information System (INIS)

    Chakravarti, A.; Noll, E.; Black, P.M.; Loeffler, J.S.

    2001-01-01

    Purpose: Increasing evidence suggests that signaling mediated by the epidermal growth factor receptor (EGFR) pathway contributes to radiation resistance. The anti-EGFR monoclonal antibody, C225, has been shown to enhance radiation response for several tumor types in preclinical models. Malignant gliomas are known to express, and quite frequently overexpress, EGFR. Our objectives in this study were to 1) Evaluate the efficacy of C225 as a radiation response modifier in EGFR-expressing glioma cell lines and to 2) Investigate the underlying molecular mechanisms mediating C225-induced enhancement of radiation response. Materials and Methods: Twelve EGFR-expressing glioma cells lines, established from patient tumors, were used for this study. Cells were incubated with C225, irradiated, and then evaluated for radiation response. Assays used to evaluate efficacy of C225-mediated radiosensitization included time-course apoptosis assays (Annexin V and TUNEL), viability assays (MTT), and clonogenic survival assays. The changes along MAPK (p44/p42)/JNK/p38-MAPK signal transduction pathways were then investigated using quantitative Western analysis with phospho-specific antibodies to determine the molecular mechanisms by which C225 mediates a given response. Results: C225 clearly enhanced radiation response for 7 of the 12 primary glioma cell lines studied. Enhancement of both immediate and delayed apoptotic responses was evident in these 7 responsive cell lines after C225 administration. The average apoptosis index at 6 hours post-RT+C225 for the 7 responsive lines was 9.5%, compared to 1.2% for the RT-only controls. A pattern of delayed apoptosis was evident in these 7 lines, with secondary apoptotic peaks (∼ 8.0%) occurring at 24 hours post-RT+C225. Time course viability measurements revealed a steady decrease in viable tumor cells in these responsive cell lines from 75% at 6 hours post-RT+C225 to 20% at 7 days. Clonogenic survival was also diminished in these 7 lines

  5. Estimating the location and shape of hybrid zones

    DEFF Research Database (Denmark)

    Guedj, Benjamin; Guillot, Gilles

    2011-01-01

    We propose a new model to make use of georeferenced genetic data for inferring the location and shape of a hybrid zone. The model output includes the posterior distribution of a parameter that quantifies the width of the hybrid zone. The model proposed is implemented in the GUI and command‐line v...

  6. Computer simulations of upper-hybrid and electron cyclotron resonance heating

    International Nuclear Information System (INIS)

    Lin, A.T.; Lin, C.C.

    1983-01-01

    A 2 1/2 -dimensional relativistic electromagnetic particle code is used to investigate the dynamic behavior of electron heating around the electron cyclotron and upper-hybrid layers when an extraordinary wave is obliquely launched from the high-field side into a magnetized plasma. With a large angle of incidence most of the radiation wave energy converts into electrostatic electron Bernstein waves at the upper-hybrid layer. These mode-converted waves propagate back to the cyclotron layer and deposit their energy in the electrons through resonant interactions dominated first by the Doppler broadening and later by the relativistic mass correction. The line shape for both mechanisms has been observed in the simulations. At a later stage, the relativistic resonance effects shift the peak of the temperature profile to the high-field side. The heating ultimately causes the extraordinary wave to be substantially absorbed by the high-energy electrons. The steep temperature gradient created by the electron cyclotron heating eventually reflects a substantial part of the incident wave energy. The diamagnetic effects due to the gradient of the mode-converted Bernstein wave pressure enhance the spreading of the electron heating from the original electron cyclotron layer

  7. A Novel Hybrid Error Criterion-Based Active Control Method for on-Line Milling Vibration Suppression with Piezoelectric Actuators and Sensors

    Directory of Open Access Journals (Sweden)

    Xingwu Zhang

    2016-01-01

    Full Text Available Milling vibration is one of the most serious factors affecting machining quality and precision. In this paper a novel hybrid error criterion-based frequency-domain LMS active control method is constructed and used for vibration suppression of milling processes by piezoelectric actuators and sensors, in which only one Fast Fourier Transform (FFT is used and no Inverse Fast Fourier Transform (IFFT is involved. The correction formulas are derived by a steepest descent procedure and the control parameters are analyzed and optimized. Then, a novel hybrid error criterion is constructed to improve the adaptability, reliability and anti-interference ability of the constructed control algorithm. Finally, based on piezoelectric actuators and acceleration sensors, a simulation of a spindle and a milling process experiment are presented to verify the proposed method. Besides, a protection program is added in the control flow to enhance the reliability of the control method in applications. The simulation and experiment results indicate that the proposed method is an effective and reliable way for on-line vibration suppression, and the machining quality can be obviously improved.

  8. Radiation Effect on Body Weight and Hematological Changes of Hybrid Mice by Conventional Fraction, Large Abdominal Field Irradiation

    International Nuclear Information System (INIS)

    Lee, Sung Heon; Shin, Sei One; Kim, Myung Se

    1985-01-01

    Radiation effect on mammals, especially on hematologic changes, has been studied since discovery of x-ray. Various experimental animals were tried for radiobiological studies. 72 hybrid mice with conventional fraction (5X/week), large abdominal field (2 x 3cm, from symphysis pubic to xyphoid process) were used. Body weight was declined gradually by increasing irradiation doses, nadir was about 29.7% in male ; 30.4% in female at 6000 rad irradiation group. Hemoglobin value was nearly normal throughout entire treatment. Significant dropping of WBC count was noted to 40-50% of pretreatment values by only 1000 rad irradiation. Change of differential count was interesting; lymphocyte proportion showed gradual reduction, instead of gradual increasing of segmented neutrophil. Those proportion were reversed after 6000 rad irradiation. Urinary protein tests showed + - +++, showing no correlation with dosage. Application. of our study in clinical combination therapy (radiation + chemotherapy) was discussed

  9. Design of a hybrid emissivity domestic electric oven

    Science.gov (United States)

    Isik, Ozgur; Onbasioglu, Seyhan Uygur

    2017-10-01

    In this study, the radiative properties of the surfaces of an electric oven were investigated. Using experimental data related to an oven-like enclosure, a novel combination of surface properties was developed. Three different surface emissivity combinations were analysed experimentally: low-emissivity, high emissivity (black-coated), and hybrid emissivity. The term "hybrid emissivity design" here corresponds to an enclosure with some high emissive and some low-emissive surfaces. The experiments were carried out according to the EN 50304 standard. When a brick (load) was placed in the enclosure, the view factors between its surfaces were calculated with the Monte Carlo method. These and the measured surface temperatures were then used to calculate the radiative heat fluxes on the surfaces of the load. The three different models were compared with respect to energy consumption and baking time. The hybrid model performed best, with the highest radiative heat transfer between the surfaces of the enclosure and the load and minimum heat loss from the cavity. Thus, it was the most efficient model with the lowest energy consumption and the shortest baking time. The recent European Union regulation regarding the energy labelling of domestic ovens was used.

  10. Measuring 20-100 T B-fields using Zeeman splitting of sodium emission lines on a 500 kA pulsed power machine

    Energy Technology Data Exchange (ETDEWEB)

    Banasek, J. T., E-mail: jtb254@cornell.edu; Engelbrecht, J. T.; Pikuz, S. A.; Shelkovenko, T. A.; Hammer, D. A. [Cornell University, Ithaca, New York 14850 (United States)

    2016-11-15

    We have shown that Zeeman splitting of the sodium (Na) D-lines at 5890 and 5896 Å can be used to measure the magnetic field (B-field) produced in high current pulsed power experiments. We have measured the B-field next to a return current conductor in a hybrid X-pinch experiment near a peak current of about 500 kA. Na is deposited on the conductor and then is desorbed and excited by radiation from the hybrid X-pinch. The D-line emission spectrum implies B-fields of about 20 T with a return current post of 4 mm diameter or up to 120 T with a return current wire of 0.455 mm diameter. These measurements were consistent or lower than the expected B-field, thereby showing that basic Zeeman splitting can be used to measure the B-field in a pulsed-power-driven high-energy-density (HED) plasma experiment. We hope to extend these measurement techniques using suitable ionized species to measurements within HED plasmas.

  11. An empirical line-by-line model for the infrared solar transmittance spectrum from 700 to 5000cm{sup -1}

    Energy Technology Data Exchange (ETDEWEB)

    Hase, F. [Institut fuer Meteorologie und Klimaforschung, Forschungszentrum Karlsruhe, Postfach 3640, D-76021 Karlsruhe (Germany)]. E-mail: frank.hase@imk.fzk.de; Demoulin, P. [Institut d' Astrophysique et de Geophysique, allee du VI aout, 17, batiment B5a, B-4000, Liege (Belgium); Sauval, A.J. [Observatoire Royal de Belgique, avenue circulaire, 3, B-1180, Bruxelles (Belgium); Toon, G.C. [Jet Propulsion Laboratory, 4800 Oak Grove Drive, Pasadena, CA 91109 (United States); Bernath, P.F. [Department of Chemistry, University of Waterloo, Waterloo, Ont., Canada N2L3G1 (Canada); Goldman, A. [Department of Physics, University of Denver, Denver, CO 80208 (United States); Hannigan, J.W. [Atmospheric Chemistry Division, National Center for Atmospheric Research, P.O. Box 3000, Boulder, CO 80303 (United States); Rinsland, C.P. [NASA Langley Research Center, Hampton, VA 23681-2199 (United States)

    2006-12-15

    An empirical line-by-line model for the infrared solar transmittance spectrum is presented. The model can be incorporated into radiative transfer codes to allow fast calculation of all relevant emission and absorption features in the solar spectrum in the mid-infrared region from 700 to 5000cm{sup -1}. The transmittance is modelled as a function of the diameter of the field-of-view centered on the solar disk: the line broadening due to solar rotation as well as center-to-limb variations in strength and width are taken into account for stronger lines. Applications of the model presented here are in the fields of terrestrial remote sensing in the mid-infrared spectral region when the sun is used as radiation source or scattered solar radiation contributes to the measured signal and in the fields of atmospheric radiative transfer algorithms which compute the propagation of infrared solar radiation in the terrestrial atmosphere.

  12. Trichostatin A, a histone deacetylase inhibitor, potentiated cytotoxic effect of lionizing radiation in human head and neck cancer cell lines

    International Nuclear Information System (INIS)

    Kim, Jin Ho; Shin, Jin Hee; Chie, Eui Kyu; Wu, Hong Gyun; Kim, Jae Sung; Kim, Il Han; Ha, Sung Whan; Park, Charn Il; Kang, Wee Saing

    2004-01-01

    We have previously reported that human glioblastoma cells are sensitized to radiation-induced death after their exposure to trichostatin A (TSA), a histone deacetylase inhibitor (HDAC-I), prior to the irradiation. We aimed to measure the magnitude of the radiosensitizing effect of TSA in human head and neck cancer cell lines. human head and neck cancer cell lines, HN-3 and HN-9, were exposed to 0, 50, 100, and 200 nM TSA for 18 hr prior to irradiation. Then, the TSA-treated cells were irradiated with 0, 2, 4, 6, and 8 Gy, and cell survival was measured by clonogenic assay. Pre-irradiation exposure to TSA was found to radiosensitize HN-3 and HN-9 cell lines. In HN-9 cells, the fraction surviving after 2 Gy (SF2) was significantly reduced by treatment of TSA at concentration as low as 50 nM. However, a treatment with 200 nM TSA was required to significantly decrease SF2 in the HN-3 cell line. SER of pre-irradiation treatment with 200 nM TSA was 1.84 in HN-3 and 7.24 in HN-9, respectively. Our results clearly showed that human head and neck cancer cell lines can be sensitized to ionizing radiation by pre-irradiation inhibition of histone deacetylase (HDAC) using TSA, and that this potentiation might well be a general phenomenon

  13. Suppression or knockout of SaF/SaM overcomes the Sa-mediated hybrid male sterility in rice.

    Science.gov (United States)

    Xie, Yongyao; Niu, Baixiao; Long, Yunming; Li, Gousi; Tang, Jintao; Zhang, Yaling; Ren, Ding; Liu, Yao-Guang; Chen, Letian

    2017-09-01

    Hybrids between the indica and japonica subspecies of rice (Oryza sativa) are usually sterile, which hinders utilization of heterosis in the inter-subspecific hybrid breeding. The complex locus Sa comprises two adjacently located genes, SaF and SaM, which interact to cause abortion of pollen grains carrying the japonica allele in japonica-indica hybrids. Here we showed that silencing of SaF or SaM by RNA interference restored male fertility in indica-japonica hybrids with heterozygous Sa. We further used clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9-based genome editing to knockout the SaF and SaM alleles, respectively, of an indica rice line to create hybrid-compatible lines. The resultant artificial neutral alleles did not affect pollen viability and other agricultural traits, but did break down the reproductive barrier in the hybrids. We found that some rice lines have natural neutral allele Sa-n, which was compatible with the typical japonica or indica Sa alleles in hybrids. Our results demonstrate that SaF and SaM are required for hybrid male sterility, but are not essential for pollen development. This study provides effective approaches for the generation of hybrid-compatible lines by knocking out the Sa locus or using the natural Sa-n allele to overcome hybrid male sterility in rice breeding. © 2017 The Authors. Bioelectromagnetics published by Wiley Periodicals, Inc. © 2017 Institute of Botany, Chinese Academy of Sciences.

  14. Establishment of immortalized B lymphoblastoid cell lines of old residents in high background radiation area in Guangdong, China

    International Nuclear Information System (INIS)

    Lu Xue; Feng Jiangbing; Chen Deqing; Liu Qingjie; Cha Yongru; Zou Jianming

    2008-01-01

    Objective: To establish the immortalized cell lines of peripheral blood lymphocytes for old male residents in high background radiation area (HBRA) in Guangdong, China, in order to preserve the specific genomic resources of residents in HBRA for the further genetic and molecular biological study on HBRA. Methods: The peripheral blood samples of 20 old male residents in HBRA were collected after informed consent. The immortalized B lymphoblastoid cell lines, 2 fox each resident, were established with Epstein-Barr virus. After being frozen and recovered, the cell viability, the contamination of bacterium and mycoplasma were analyzed. The stabilization of cell lines was decided by comparing the karyotypes of the peripheral blood lymphocytes and the cell lines. Results: 40 cell lines for 20 residents in HBRA were successfully established.. The recovery rate of cell lines after being frozen was 100% . All the cell viablity after recovery was higher than 90%, and no contamination of bacteria and mycoplasma occurred. The karyotypes of the 20th generation cell lines were not change. Conclusion: The immortalized cell lines established in this study could provide biological resources for further study on genetics and molecular biology in HBRA. (authors)

  15. A hybrid transport-diffusion method for Monte Carlo radiative-transfer simulations

    International Nuclear Information System (INIS)

    Densmore, Jeffery D.; Urbatsch, Todd J.; Evans, Thomas M.; Buksas, Michael W.

    2007-01-01

    Discrete Diffusion Monte Carlo (DDMC) is a technique for increasing the efficiency of Monte Carlo particle-transport simulations in diffusive media. If standard Monte Carlo is used in such media, particle histories will consist of many small steps, resulting in a computationally expensive calculation. In DDMC, particles take discrete steps between spatial cells according to a discretized diffusion equation. Each discrete step replaces many small Monte Carlo steps, thus increasing the efficiency of the simulation. In addition, given that DDMC is based on a diffusion equation, it should produce accurate solutions if used judiciously. In practice, DDMC is combined with standard Monte Carlo to form a hybrid transport-diffusion method that can accurately simulate problems with both diffusive and non-diffusive regions. In this paper, we extend previously developed DDMC techniques in several ways that improve the accuracy and utility of DDMC for nonlinear, time-dependent, radiative-transfer calculations. The use of DDMC in these types of problems is advantageous since, due to the underlying linearizations, optically thick regions appear to be diffusive. First, we employ a diffusion equation that is discretized in space but is continuous in time. Not only is this methodology theoretically more accurate than temporally discretized DDMC techniques, but it also has the benefit that a particle's time is always known. Thus, there is no ambiguity regarding what time to assign a particle that leaves an optically thick region (where DDMC is used) and begins transporting by standard Monte Carlo in an optically thin region. Also, we treat the interface between optically thick and optically thin regions with an improved method, based on the asymptotic diffusion-limit boundary condition, that can produce accurate results regardless of the angular distribution of the incident Monte Carlo particles. Finally, we develop a technique for estimating radiation momentum deposition during the

  16. Failed Radiatively Accelerated Dusty Outflow Model of the Broad Line Region in Active Galactic Nuclei. I. Analytical Solution

    Energy Technology Data Exchange (ETDEWEB)

    Czerny, B.; Panda, S.; Wildy, C.; Sniegowska, M. [Center for Theoretical Physics, Polish Academy of Sciences, Al. Lotników 32/46, 02-668 Warsaw (Poland); Li, Yan-Rong; Wang, J.-M. [Key Laboratory for Particle Astrophysics, Institute of High Energy Physics, Chinese Academy of Sciences, 19B Yuquan Road, Beijing 100049 (China); Hryniewicz, K.; Sredzinska, J. [Copernicus Astronomical Center, Polish Academy of Sciences, Bartycka 18, 00-716 Warsaw (Poland); Karas, V., E-mail: bcz@cft.edu.pl [Astronomical Institute, Academy of Sciences, Bocni II 1401, CZ-141 00 Prague (Czech Republic)

    2017-09-10

    The physical origin of the broad line region in active galactic nuclei is still unclear despite many years of observational studies. The reason is that the region is unresolved, and the reverberation mapping results imply a complex velocity field. We adopt a theory-motivated approach to identify the principal mechanism responsible for this complex phenomenon. We consider the possibility that the role of dust is essential. We assume that the local radiation pressure acting on the dust in the accretion disk atmosphere launches the outflow of material, but higher above the disk the irradiation from the central parts causes dust evaporation and a subsequent fallback. This failed radiatively accelerated dusty outflow is expected to represent the material forming low ionization lines. In this paper we formulate simple analytical equations to describe the cloud motion, including the evaporation phase. The model is fully described just by the basic parameters of black hole mass, accretion rate, black hole spin, and viewing angle. We study how the spectral line generic profiles correspond to this dynamic. We show that the virial factor calculated from our model strongly depends on the black hole mass in the case of enhanced dust opacity, and thus it then correlates with the line width. This could explain why the virial factor measured in galaxies with pseudobulges differs from that obtained from objects with classical bulges, although the trend predicted by the current version of the model is opposite to the observed trend.

  17. Chromatin status of apoptosis genes correlates with sensitivity to chemo-, immune- and radiation therapy in colorectal cancer cell lines.

    Science.gov (United States)

    Benard, Anne; Janssen, Connie M; van den Elsen, Peter J; van Eggermond, Marja C J A; Hoon, Dave S B; van de Velde, Cornelis J H; Kuppen, Peter J K

    2014-12-01

    The apoptosis pathway of programmed cell death is frequently deregulated in cancer. An intact apoptosis pathway is required for proper response to anti-cancer treatment. We investigated the chromatin status of key apoptosis genes in the apoptosis pathway in colorectal cancer cell lines in relation to apoptosis induced by chemo-, immune- or radiation therapy. Using chromatin immunoprecipitation (ChIP), we measured the presence of transcription-activating histone modifications H3Ac and H3K4me3 and silencing modifications H3K9me3 and H3K27me3 at the gene promoter regions of key apoptosis genes Bax, Bcl2, Caspase-9, Fas (CD95) and p53. Cell lines DLD1, SW620, Colo320, Caco2, Lovo and HT29 were treated with cisplatin, anti-Fas or radiation. The apoptotic response was measured by flow cytometry using propidium iodide and annexin V-FITC. The chromatin status of the apoptosis genes reflected the activation status of the intrinsic (Bax, Bcl2, Caspase-9 and p53) and extrinsic (Fas) pathways. An active intrinsic apoptotic pathway corresponded to sensitivity to cisplatin and radiation treatment of cell lines DLD1, SW620 and Colo320. An active Fas promoter corresponded to an active extrinsic apoptotic pathway in cell line DLD1. mRNA expression data correlated with the chromatin status of the apoptosis genes as measured by ChIP. In conclusion, the results presented in this study indicate that the balance between activating and silencing histone modifications, reflecting the chromatin status of apoptosis genes, can be used to predict the response of tumor cells to different anti-cancer therapies and could provide a novel target to sensitize tumors to obtain adequate treatment responses.

  18. Hybrid fission-fusion nuclear reactors

    International Nuclear Information System (INIS)

    Zucchetti, Massimo

    2011-01-01

    A fusion-fission hybrid could contribute to all components of nuclear power - fuel supply, electricity production, and waste management. The idea of the fusion-fission hybrid is many decades old. Several ideas, both new and revisited, have been investigated by hybrid proponents. These ideas appear to have attractive features, but they require various levels of advances in plasma science and fusion and nuclear technology. As a first step towards the development of hybrid reactors, fusion neutron sources can be considered as an option. Compact high-field tokamaks can be a candidate for being the neutron source in a fission-fusion hybrid, essentially due to their design characteristics, such as compact dimensions, high magnetic field, flexibility of operation. This study presents the development of a tokamak neutron source for a material testing facility using an Ignitor-based concept. The computed values show the potential of this neutron-rich device for fusion materials testing. Some full-power months of operation are sufficient to obtain relevant radiation damage values in terms of dpa. (Author)

  19. Proteomic profiling of eggs from a hybrid abalone and its parental lines: Haliotis discus hannai Ino and Haliotis gigantea.

    Science.gov (United States)

    Di, Guilan; Luo, Xuan; Huang, Miaoqin; Chen, Jun; Kong, Xianghui; Miao, Xiulian; Ke, Caihuan

    2015-12-01

    Proteomic analysis was performed on the eggs of hybrid abalone and their corresponding parental lines. A total of 915 ± 19 stained protein spots were detected from Haliotis discus hannai♀ × H. discus hannai♂ (DD), 935 ± 16 from H. gigantea♀ × H. gigantea♂ (GG) and 923 ± 13 from H. gigantea♀ × H. discus hannai♂ (GD). The spots from DD and GD were clustered together. The distance between DD and GG was maximal by hierarchical cluster analysis. A total of 112 protein gel spots were identified; of these, 59 were abalone proteins. The proteins were involved in major biological processes including energy metabolism, proliferation, apoptosis, signal transduction, immunity, lipid metabolism, electron carrier proteins, protein biosynthesis and decomposition, and cytoskeletal structure. Three of 20 differential expression protein spots involved in energy metabolism exhibited as upregulated in GD, 13 spots exhibited additivity, and four spots exhibited as downregulated in the offspring. Eleven protein spots were expressed at the highest level in DD. The proteins involved in stress responses included superoxide dismutase, peroxiredoxin 6, thioredoxin peroxidase and glutathione-S-transferase. Two of seven differential expression protein spots involved in response to stress exhibited as upregulated in GD, three exhibited additivity, and two exhibited as downregulated. These results might suggest that proteomic approaches are suitable for the analysis of hybrids and the functional prediction of abalone hybridization. © 2015 Stichting International Foundation for Animal Genetics.

  20. Genetic determination of high productivity in experimental hybrid combinations of sugar beet (Beta vulgaris L.

    Directory of Open Access Journals (Sweden)

    М. О. Корнєєва

    2016-05-01

    Full Text Available Purpose. Creation of experimental sugar beet hybrid combinations of high sugar yield values and defining gene­tic determination of their heterotic effect. Methods. Diallel crossing and topcrossing, genetic analysis of quantitative traits. Results. The authors have studied the frequency of occurrence of sugar beet heterotic hybrid combinations for «sugar yield» trait created on the basis of two pollinator lines to be genetically valuable for productivity elements, CMS lines and single-cross sterile hybrids with the use of diallel and topcrossing system of controlled hybridization. The share of parental components’ effect and their interaction in CMS hybrids variability for productivity was determined. Expediency of heterotic forecasting based on high combining ability lines was substabtiated. Promising high-yielding sugar beet combinations were selected that exceeded the group standard by 4.1–16.3%. Conclusions. The theory of genetic balance by M. V. Turbin was confirmed. Such hybrids as [CMS 5OT 4]MGP 1 (116.3%, [CMS 1OT 2]MGP 1 (112.5% and [CMS 3OT 5]MGP 1 (113.2% were recognized as the best for their productivity, MGP 1 and MGP 2 lines – as the best for their combining ability.

  1. Measurements of downwelling far-infrared radiance during the RHUBC-II campaign at Cerro Toco, Chile and comparisons with line-by-line radiative transfer calculations

    Science.gov (United States)

    Mast, Jeffrey C.; Mlynczak, Martin G.; Cageao, Richard P.; Kratz, David P.; Latvakoski, Harri; Johnson, David G.; Turner, David D.; Mlawer, Eli J.

    2017-09-01

    Downwelling radiances at the Earth's surface measured by the Far-Infrared Spectroscopy of the Troposphere (FIRST) instrument in an environment with integrated precipitable water (IPW) as low as 0.03 cm are compared with calculated spectra in the far-infrared and mid-infrared. FIRST (a Fourier transform spectrometer) was deployed from August through October 2009 at 5.38 km MSL on Cerro Toco, a mountain in the Atacama Desert of Chile. There FIRST took part in the Radiative Heating in Unexplored Bands Campaign Part 2 (RHUBC-II), the goal of which is the assessment of water vapor spectroscopy. Radiosonde water vapor and temperature vertical profiles are input into the Atmospheric and Environmental Research (AER) Line-by-Line Radiative Transfer Model (LBLRTM) to compute modeled radiances. The LBLRTM minus FIRST residual spectrum is calculated to assess agreement. Uncertainties (1-σ) in both the measured and modeled radiances are also determined. Measured and modeled radiances nearly all agree to within combined (total) uncertainties. Features exceeding uncertainties can be corrected into the combined uncertainty by increasing water vapor and model continuum absorption, however this may not be necessary due to 1-σ uncertainties (68% confidence). Furthermore, the uncertainty in the measurement-model residual is very large and no additional information on the adequacy of current water vapor spectral line or continuum absorption parameters may be derived. Similar future experiments in similarly cold and dry environments will require absolute accuracy of 0.1% of a 273 K blackbody in radiance and water vapor accuracy of ∼3% in the profile layers contributing to downwelling radiance at the surface.

  2. Analysis of DNA repair in XP-HeLa hybrids; lack of correlation between excision repair of u.v. damage and adenovirus reactivation in an XP(D)-like cell line

    International Nuclear Information System (INIS)

    Johnson, R.Y.; Squires, S.; Elliott, G.C.

    1986-01-01

    Hybrids formed between HeLa cells and fibroblasts from xeroderma pigmentosum group D show either HeLa sensitivity or XPD-like hypersensitivity to u.v. radiation and corresponding high or low excision repair capability. Hybrids with low repair are presumed to have lost, via chromosome segregation, the HeLa wild type D alleles. The u.v. sensitivity and excision repair capability of another hybrid, HD1A, derived spontaneously from the normally sensitive hybrid HD1 are analyzed. While HD1A closely resembles the XPD phenotype in terms of u.v. sensitivity and excision repair it differs from XPD because of its ability to reactivate u.v.-irradiated adenovirus 2 to an extent similar to that of its HeLa parent. This capacity functionally dissociates excision repair of chromatin-based damage from damage in a viral environment. Moreover, on the basis of complementation studies the excision repair of genomic damage by HD1A is subtly different from that of a true XPD-like hybrid, HD2. The data are discussed in terms of a second change in the defective D allele of the HD1A cell. (author)

  3. Power line harmonic radiation observed by the DEMETER spacecraft at 50/60Hz and low harmonics

    Czech Academy of Sciences Publication Activity Database

    Němec, F.; Parrot, M.; Santolík, Ondřej

    2015-01-01

    Roč. 120, č. 10 (2015), s. 8954-8967 ISSN 2169-9380 R&D Projects: GA ČR(CZ) GA14-31899S; GA MŠk LH12231 Institutional support: RVO:68378289 Keywords : PLHR * power line harmonic radiation * DEMETER Subject RIV: BL - Plasma and Gas Discharge Physics Impact factor: 3.318, year: 2015 http://onlinelibrary.wiley.com/doi/10.1002/2015JA021682/full

  4. induced chromosome aberrations analyzed by fluorescence in situ hybridization. Eight years follow up of the Goiania radiation accident victims

    International Nuclear Information System (INIS)

    Natarajan, A.T.; Santos, S.J.; Darroudi, F.; Hadjidikova, V.; Vermeulen, S.; Chatterjee, S.; Van de Berg, M.; Grigorova, M.; Sakamoto-Hojo, E.T.; Granath, F.; Ramalho, A.T.; Curado, M.P.

    1998-01-01

    The radiation accident in focus here occurred in a section of Goiania (Brazil) where more than a hundred individuals were contaminated with on September 1987. In order to estimate the absorbed radiation doses, initial frequencies of dicentrics and rings were determined in 129 victims [A.T. Ramalho, PhD Thesis, Subsidios a tecnica de dosimetria citogenetica gerados a partir da analise de resultados obtidos com o acidente radiologico de Goiania, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil, 1992]. We have followed some of these victims cytogenetically over the years seeking for parameters that could be used as basis for retrospective radiation dosimetry. Our data on translocation frequencies obtained by fluorescence in situ hybridization (FISH) could be directly compared to the baseline frequencies of dicentrics available for those same victims. Our results provided valuable information on how precise these estimates are. The frequencies of translocations observed years after the radiation exposure were two to three times lower than the initial dicentrics frequencies, the differences being larger at higher doses (>1 Gy). The accuracy of such dose estimates might be increased by scoring sufficient amount of cells. However, factors such as the persistence of translocation carrying lymphocytes, translocation levels not proportional to chromosome size, and inter-individual variation reduce the precision of these estimates

  5. Induced chromosome aberrations analyzed by fluorescence in situ hybridization. Eight years follow up of the Goiania radiation accident victims

    Energy Technology Data Exchange (ETDEWEB)

    Natarajan, A.T.; Santos, S.J.; Darroudi, F.; Hadjidikova, V.; Vermeulen, S.; Chatterjee, S.; Van de Berg, M.; Grigorova, M. [Leiden University Medical Centrum LUMC, Department of Radiation Genetics and Chemical Mutagenesis, Wassenaarseweg 72, 2333 AL Leiden (Netherlands); Sakamoto-Hojo, E.T. [Department of Biology, Faculty of Philosophy, Sciences and Letters of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto (Brazil); Granath, F. [Department of Mathematical Statistics, Stockholm University, Stockholm (Sweden); Ramalho, A.T. [Institute of Radioprotection and Dosimetry, National Commission of Nuclear Energy, Rio de Janeiro (Brazil); Curado, M.P. [Foundation Leide das Neves Ferreira, Goiania (Brazil)

    1998-05-25

    The radiation accident in focus here occurred in a section of Goiania (Brazil) where more than a hundred individuals were contaminated with on September 1987. In order to estimate the absorbed radiation doses, initial frequencies of dicentrics and rings were determined in 129 victims [A.T. Ramalho, PhD Thesis, Subsidios a tecnica de dosimetria citogenetica gerados a partir da analise de resultados obtidos com o acidente radiologico de Goiania, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil, 1992]. We have followed some of these victims cytogenetically over the years seeking for parameters that could be used as basis for retrospective radiation dosimetry. Our data on translocation frequencies obtained by fluorescence in situ hybridization (FISH) could be directly compared to the baseline frequencies of dicentrics available for those same victims. Our results provided valuable information on how precise these estimates are. The frequencies of translocations observed years after the radiation exposure were two to three times lower than the initial dicentrics frequencies, the differences being larger at higher doses (>1 Gy). The accuracy of such dose estimates might be increased by scoring sufficient amount of cells. However, factors such as the persistence of translocation carrying lymphocytes, translocation levels not proportional to chromosome size, and inter-individual variation reduce the precision of these estimates

  6. Main biological characters of series of mutant waxy rices developed from irradiation-induced mutation

    International Nuclear Information System (INIS)

    Huang Ronghua; Zhang Shubiao; Zhang Qingqi; Yang Rencui; Lin Jinhu

    2008-01-01

    The main biological characters of the waxy male sterile lines, maintainer lines, restorer lines and waxy hybrids which had been developed by radiation-induced mutation were studied, and the grain quality of the waxy hybrids were analyzed as well. Sesults indicated that the mutant waxy rice had the same growth duration, similar agronomic characters, panicle and spikelet traits as parent. The waxy male-sterile line had the same pollen sterility characteristic as its parent male-sterile line. The waxy hybrid rice retained the yield potential as original hybrid rice, and the grain quality of the waxy hybrids was similar to the conventional waxy rice Ejinnuo 6. (authors)

  7. High peak current operation of x-ray free-electron laser multiple beam lines by suppressing coherent synchrotron radiation effects

    Science.gov (United States)

    Hara, Toru; Kondo, Chikara; Inagaki, Takahiro; Togawa, Kazuaki; Fukami, Kenji; Nakazawa, Shingo; Hasegawa, Taichi; Morimoto, Osamu; Yoshioka, Masamichi; Maesaka, Hirokazu; Otake, Yuji; Tanaka, Hitoshi

    2018-04-01

    The parallel operation of multiple beam lines is an important means to expand the opportunity of user experiments at x-ray free-electron laser (XFEL) facilities. At SPring-8 Angstrom free-electron laser (SACLA), the multi-beam-line operation had been tested using two beam lines, but transverse coherent synchrotron radiation (CSR) effects at a dogleg beam transport severely limited the laser performance. To suppress the CSR effects, a new beam optics based on two double bend achromat (DBA) structures was introduced for the dogleg. After the replacement of the beam optics, high peak current bunches of more than 10 kA are now stably transported through the dogleg and the laser pulse output is increased by a factor of 2-3. In the multi-beam-line operation of SACLA, the electron beam parameters, such as the beam energy and peak current, can be adjusted independently for each beam line. Thus the laser output can be optimized and wide spectral tunability is ensured for all beam lines.

  8. Interfering line in trace analysis by X-ray spectrometry: Radiative auger satellites

    International Nuclear Information System (INIS)

    Maeda, Kuniko; Kawai, Jun.

    1994-01-01

    Strong characteristic X-ray lines (e.g. Kα and Kβ) are accompanied by broad low-energy satellites caused by the radiative Auger effect (RAE). In order to prove how the RAE satellites interfere the analysis of minor elements, low-energy side spectra of Ca and Ti Kβ, and Ca-Fe Kα were measured. The obtained RAE intensities are summarized together with published experimental and theoretical data. The integrated intensities of satellites due to K → MM, K → LM and K → LL RAE relative to that of Kα are determined to be of the order of 0.01-0.1%. This warns that the neglecting of the RAE satellites will introduce a serious error in trace analysis. (author)

  9. Using RADMC-3D to model the radiative transfer of spectral lines in protoplanetary disks and envelopes

    Science.gov (United States)

    DeVries, John; Terebey, Susan

    2018-06-01

    Protoplanetary disks are the birthplaces of planets in our universe. Observations of these disks with radio telescopes like the Atacama Large Millimeter Array (ALMA) offer great insight into the star and planet formation process. Comparing theories of formation with observations requires tracing the energy transfer via electromagnetic radiation, known as radiative transfer. To determine the temperature distribution of circumstellar material, a Monte Carlo code (Whitney et al. [1]) was used to to perform the radiative transfer through dust. The goal of this research is to utilize RADMC-3D [2] to handle the spectral line radiative transfer computations. An existing model of a rotating ring was expanded to include emission from the C18O isotopologue of carbon monoxide using data from the Leiden Atomic and Molecular Database (LAMDA). This feature of our model compliments ALMA's ability to measure C18O line emission, a proxy for disk rotation. In addition to modeling gas in the protoplanetary disk, dust also plays an important role. The generic description of absorption and scattering for dust provided by RADMC-3D was changed in favor of a more physically-realistic description with OH5 grains. This description is more appropriate in high-density regions of the envelope around a protostar. Further improvements, such as consideration for the finite resolution of observations, have been implemented. The task at present is to compare our model with observations of protoplanetary systems like L1527. Some results of these comparisons will be presented.[1] Whitney et al. 2013, ApJS, 207:30[2] RADMC-3D: http://www.ita.uni-heidelberg.de/~dullemond/software/radmc-3d/

  10. Influence of Laser Radiation Power Density on the Intensity of Spectral Lines for Main Components in a Clay Laser-Induced Plasma

    Science.gov (United States)

    Anufrik, S. S.; Kurian, N. N.; Znosko, K. F.; Belkov, M. V.

    2018-05-01

    We have studied the intensity of the spectral lines for the main components in clay: Al I 309.4 nm, Al II 358.7 nm, Mg II 279.6 nm, Ti II 323.6 nm vs. the position of the object relative to the focus of the optical system when the samples are exposed to single laser pulses from a YAG:Nd3+ laser. We have determined the permissible ranges for positioning the object relative to the focus of the optical system (positive and negative defocusing) for which there is practically no change in the reproducibility of the intensity for the spectral lines for red and white clay samples. We show that the position of the object relative to the focus of the optical system should be within the range ΔZ ±1.5 mm for optimal laser pulse energies for the analyte spectral lines. We have calculated the radiation flux density for different laser pulse energies and different distances from the focus to the object. We have shown experimentally that reducing the radiation flux density leads to a decrease in the intensity of the analyte spectral lines.

  11. Radiating properties of solar plasmas

    Science.gov (United States)

    Bruner, M. E.; Mcwhirter, R. W. P.

    1988-01-01

    Using a series of 14 previously obtained empirical emission measure distributions and a number of spectral lines observed by the SMM and P78-1 instruments, the total power radiated by a hot plasma is compared to that radiated by individual spectrum lines. Results are presented for different choices of ionization balance and power loss functions. The results indicate that for some lines such as the C IV resonance doublet at 1548 A and 1550 A, the ratio of the line intensity to the total radiated power varied only over a factor of 2, suggesting that well-calibrated measurements of a single line intensity may provide a fairly good estimation of the total radiated power output from the solar plasma.

  12. Radiating properties of solar plasmas

    International Nuclear Information System (INIS)

    Bruner, M.E.; Mcwhirter, R.W.P.

    1988-01-01

    Using a series of 14 previously obtained empirical emission measure distributions and a number of spectral lines observed by the SMM and P78-1 instruments, the total power radiated by a hot plasma is compared to that radiated by individual spectrum lines. Results are presented for different choices of ionization balance and power loss functions. The results indicate that for some lines such as the C IV resonance doublet at 1548 A and 1550 A, the ratio of the line intensity to the total radiated power varied only over a factor of 2, suggesting that well-calibrated measurements of a single line intensity may provide a fairly good estimation of the total radiated power output from the solar plasma. 21 references

  13. Development of a Handheld Line Information Reader and Generator for Efficient Management of Optical Communication Lines.

    Science.gov (United States)

    Lee, Jaeyul; Kwon, Hyungwoo; Song, Jaewon; Jeon, Mansik; Kim, Jeehyun

    2017-08-24

    A handheld line information reader and a line information generator were developed for the efficient management of optical communication lines. The line information reader consists of a photo diode, trans-impedance amplifier, voltage amplifier, microcontroller unit, display panel, and communication modules. The line information generator consists of a laser diode, laser driving circuits, microcontroller unit, and communication modules. The line information reader can detect the optical radiation field of the test line by bending the optical fiber. To enhance the sensitivity of the line information reader, an additional lens was used with a focal length of 4.51 mm. Moreover, the simulation results obtained through BeamPROP ® software from Synopsys, Inc. demonstrated a stronger optical radiation field of the fiber due to a longer transmission wavelength and larger bending angle of the fiber. Therefore, the developed devices can be considered as useful tools for the efficient management of optical communication lines.

  14. Impacts of microphysics, radiation and environmental winds in mid-latitude and tropical squall-line systems, and their climatic implications

    International Nuclear Information System (INIS)

    Chin, Hung-Neng.

    1994-08-01

    Cloud-radiation feedback has been identified as the most important factor limiting general circulation models (GCMS) to further progress in climate change research (Cess et al., 1989). It is also regarded as a major uncertainty in estimating the impact of greenhouse gases on climate simulations. As a result, many GCMs showed high sensitivity to the treatment of clouds and cloud radiative properties. Therefore, a better understanding of cloud-radiation feedback on the large-scale environment is absolutely essential to improve the representation of cloud processes in GCMS. To this end, a cloud model with enhanced model physics is used to study the impact of cloud-radiation interactions on mesoscale convective systems (MCSs). Case studies representing a variety of convective systems are important to generalize the overall effects of anvil clouds on the large-scale environment. Our primary interesting is limited to the MCSs in an environment with substantial wind shear, such as squall-line systems, because they have longer lifetime and wider coverage to impact the earth radiation budget and climate. The objective of this study is to investigate the impacts of microphysics, radiation and environmental winds on mid-latitude continental and tropical oceanic squall-line systems. Comparisons between these two systems are presented. Recent studies indicated that the vertical shear of the environmental wind plays an important role in the formation of the anvil cloud through the tilting of MCSS. However, this process has not been represented in GCMS. A detailed investigation on the formation of anvil clouds and their relationship to cumulus portions of MCSs would help develop a better cloud parameterization for use in GCMS. Two important issues are addressed through these comparisons. First, what factors cause the differences between mid-latitude and tropical anvil clouds? Second, do these differences have climatic implications to improve our climate forecasting ability?

  15. Potencial de híbridos simples de milho para extração de linhagens Potential of maize single hybrids to generate inbred lines

    Directory of Open Access Journals (Sweden)

    Odair Bison

    2003-04-01

    Full Text Available A utilização de híbridos simples comerciais de milho é uma das opções de populações para a extração de linhagens, porque são adaptados e provavelmente concentram alta freqüência de alelos favoráveis já fixados. Mesmo nos locos que estão segregando, a freqüência de alelos favoráveis é 0,5. Assim, a identificação de populações promissoras, derivadas de híbridos simples superiores, é uma boa estratégia para aumentar a eficiência dos programas de melhoramento. As populações derivadas dos híbridos simples comerciais AG9012 e C333 foram avaliadas com o objetivo de verificar o potencial dessas para extração de linhagens superiores, por meio das estimativas de parâmetros genéticos e fenotípicos, da estimativa de m+a e a metodologia proposta por Jinks & Pooni (1976. Foram avaliadas 169 famílias S1 de cada população, durante a safra agrícola de 1999/2000, na área experimental do Departamento de Biologia da UFLA, em Lavras - MG, em látice simples 13x13, sendo as parcelas constituídas por uma linha de 3 m. As características analisadas foram incidência de Phaeosphaeria maydis em duas épocas, altura de plantas, altura de espigas e produtividade de espigas despalhadas. Foi constatado que há possibilidade de se obterem linhagens com bom desempenho per se, sendo a população derivada do C333 a mais promissora, por associar resistência a Phaeosphaeria maydis e possuir média mais alta e maior probabilidade de obtenção de linhagens superiores. A metodologia proposta por Jinks & Pooni (1976 mostrou-se mais informativa do que a estimativa de m+a para a escolha de populações, mas, quando possível, as duas podem ser utilizadas simultaneamente para auxiliar na decisão dos melhoristas.Populations derived from commercial single hybrids are one of the breeder options for inbred line extraction because of their adaptation and probable high frequency of loci with fixed favorable alleles. Even the segregating loci carry

  16. Cytogenetics and stripe rust resistance of wheat-Thinopyrum elongatum hybrid derivatives.

    Science.gov (United States)

    Li, Daiyan; Long, Dan; Li, Tinghui; Wu, Yanli; Wang, Yi; Zeng, Jian; Xu, Lili; Fan, Xing; Sha, Lina; Zhang, Haiqin; Zhou, Yonghong; Kang, Houyang

    2018-01-01

    Amphidiploids generated by distant hybridization are commonly used as genetic bridge to transfer desirable genes from wild wheat species into cultivated wheat. This method is typically used to enhance the resistance of wheat to biotic or abiotic stresses, and to increase crop yield and quality. Tetraploid Thinopyrum elongatum exhibits strong adaptability, resistance to stripe rust and Fusarium head blight, and tolerance to salt, drought, and cold. In the present study, we produced hybrid derivatives by crossing and backcrossing the Triticum durum-Th. elongatum partial amphidiploid ( Trititrigia 8801, 2 n  = 6 ×  = 42, AABBEE) with wheat cultivars common to the Sichuan Basin. By means of cytogenetic and disease resistance analyses, we identified progeny harboring alien chromosomes and measured their resistance to stripe rust. Hybrid progenies possessed chromosome numbers ranging from 40 to 47 (mean = 42.72), with 40.0% possessing 42 chromosomes. Genomic in situ hybridization revealed that the number of alien chromosomes ranged from 1 to 11. Out of the 50 of analyzed lines, five represented chromosome addition (2 n  = 44 = 42 W + 2E) and other five were chromosome substitution lines (2 n  = 42 = 40 W + 2E). Importantly, a single chromosome derived from wheat- Th. elongatum intergenomic Robertsonian translocations chromosome was occurred in 12 lines. Compared with the wheat parental cultivars ('CN16' and 'SM482'), the majority (70%) of the derivative lines were highly resistant to strains of stripe rust pathogen known to be prevalent in China. The findings suggest that these hybrid-derivative lines with stripe rust resistance could potentially be used as germplasm sources for further wheat improvement.

  17. On- and off-line monitoring of ion beam treatment

    Energy Technology Data Exchange (ETDEWEB)

    Parodi, Katia, E-mail: katia.parodi@lmu.de

    2016-02-11

    Ion beam therapy is an emerging modality for high precision radiation treatment of cancer. In comparison to conventional radiation sources (photons, electrons), ion beams feature major dosimetric advantages due to their finite range with a localized dose deposition maximum, the Bragg peak, which can be selectively adjusted in depth. However, due to several sources of treatment uncertainties, full exploitation of these dosimetric advantages in clinical practice would require the possibility to visualize the stopping position of the ions in vivo, ideally in real-time. To this aim, different imaging methods have been proposed and investigated, either pre-clinically or even clinically, based on the detection of prompt or delayed radiation following nuclear interaction of the beam with the irradiated tissue. However, the chosen or ad-hoc developed instrumentation has often relied on technologies originally conceived for different applications, thus compromising on the achievable performances for the sake of cost-effectiveness. This contribution will review major examples of used instrumentation and related performances, identifying the most promising detector developments for next generation devices especially dedicated to on-line monitoring of ion beam treatment. Moreover, it will propose an original combination of different techniques in a hybrid detection scheme, aiming to make the most of complementary imaging methods and open new perspectives of image guidance for improved precision of ion beam therapy.

  18. Epidemiology of Babesia, Anaplasma and Trypanosoma species using a new expanded reverse line blot hybridization assay.

    Science.gov (United States)

    Paoletta, Martina Soledad; López Arias, Ludmila; de la Fournière, Sofía; Guillemi, Eliana Carolina; Luciani, Carlos; Sarmiento, Néstor Fabián; Mosqueda, Juan; Farber, Marisa Diana; Wilkowsky, Silvina Elizabeth

    2018-02-01

    Vector-borne hemoparasitic infections are a major problem that affects livestock industries worldwide, particularly in tropical and subtropical regions. In this work, a reverse line blot (RLB) hybridization assay was developed for the simultaneous detection and identification of Anaplasma, Babesia and bovine trypanosomes, encompassing in this way the most relevant hemoparasites that affect cattle. A total of 186 bovine blood samples collected from two different ecoepidemiological regions of northeast Argentina, with and without tick control, were analyzed with this new RLB. High diversity of parasites, such as Babesia bovis, B. bigemina, Anaplasma marginale and three different Trypanosoma species, was found. High rates of coinfections were also detected, and significant differences were observed not only in the prevalence of parasites but also in the level of coinfections between the two analyzed areas. Regarding the Trypanosoma genus, we provide molecular evidence of the presence of T. vivax and T. theileri for the first time in Argentina. Besides, since the RLB is a prospective tool, it allowed the identification of a yet unknown bovine trypanosome which could not be assigned to any of the bovine species known so far. In the present study we provide new insights on the prevalence of several pathogens that directly impact on livestock production in Argentina. The RLB assay developed here allows to identify simultaneously numerous pathogenic species which can also be easily expanded to detect other blood borne pathogens. These characteristics make the RLB hybridization assay an essential tool for epidemiological survey of all vector-borne pathogens. Copyright © 2017 Elsevier GmbH. All rights reserved.

  19. Design and Evaluation of Autonomous Hybrid Frequency-Voltage Sensitive Load Controller

    DEFF Research Database (Denmark)

    Douglass, Philip James; Garcia-Valle, Rodrigo; Sossan, Fabrizio

    2013-01-01

    The paper introduces an algorithm for control of autonomous loads without digital communication interfaces to provide both frequency regulation and voltage regulation services. This hybrid controller can be used to enhance frequency sensitive loads to mitigate line overload arising from reduced l...... load diversity. Numerical simulations of the hybrid controller in a representative distribution system show the peak system load was reduced by 12% compared to a purely frequency sensitive load controller.......The paper introduces an algorithm for control of autonomous loads without digital communication interfaces to provide both frequency regulation and voltage regulation services. This hybrid controller can be used to enhance frequency sensitive loads to mitigate line overload arising from reduced...

  20. Hybrid power pack for sustainability and environmental friendliness in driving multiple units; Hybrid-Powerpack fuer nachhaltigen und umweltfreundlichen Triebwagenantrieb

    Energy Technology Data Exchange (ETDEWEB)

    Lehmann, Ingo; Schmalzing, Claus-Oliver [MTU Friedrichshafen GmbH, Friedrichshafen (Germany); Werner, Claus [DB RegioNetz Verkehrs GmbH (Germany); Bold, Uwe [DB Systemtechnik Engineering, Kassel (Germany)

    2011-07-01

    With MTU's hybrid drive, it is possible to obtain a saving of up to 25% in fuel consumption and to operate in such a way as to avoid emissions in stations. MTU Friedrichshafen and Deutsche Bahn are testing a parallel hybrid drive on the line between Aschaffenburg und Miltenberg, starting in autumn 2011. This test is intended to produce evidence that this technology is ready for everyday use. (orig.)

  1. Review of Hybrid (Deterministic/Monte Carlo) Radiation Transport Methods, Codes, and Applications at Oak Ridge National Laboratory

    International Nuclear Information System (INIS)

    Wagner, John C.; Peplow, Douglas E.; Mosher, Scott W.; Evans, Thomas M.

    2010-01-01

    This paper provides a review of the hybrid (Monte Carlo/deterministic) radiation transport methods and codes used at the Oak Ridge National Laboratory and examples of their application for increasing the efficiency of real-world, fixed-source Monte Carlo analyses. The two principal hybrid methods are (1) Consistent Adjoint Driven Importance Sampling (CADIS) for optimization of a localized detector (tally) region (e.g., flux, dose, or reaction rate at a particular location) and (2) Forward Weighted CADIS (FW-CADIS) for optimizing distributions (e.g., mesh tallies over all or part of the problem space) or multiple localized detector regions (e.g., simultaneous optimization of two or more localized tally regions). The two methods have been implemented and automated in both the MAVRIC sequence of SCALE 6 and ADVANTG, a code that works with the MCNP code. As implemented, the methods utilize the results of approximate, fast-running 3-D discrete ordinates transport calculations (with the Denovo code) to generate consistent space- and energy-dependent source and transport (weight windows) biasing parameters. These methods and codes have been applied to many relevant and challenging problems, including calculations of PWR ex-core thermal detector response, dose rates throughout an entire PWR facility, site boundary dose from arrays of commercial spent fuel storage casks, radiation fields for criticality accident alarm system placement, and detector response for special nuclear material detection scenarios and nuclear well-logging tools. Substantial computational speed-ups, generally O(10 2-4 ), have been realized for all applications to date. This paper provides a brief review of the methods, their implementation, results of their application, and current development activities, as well as a considerable list of references for readers seeking more information about the methods and/or their applications.

  2. Review of Hybrid (Deterministic/Monte Carlo) Radiation Transport Methods, Codes, and Applications at Oak Ridge National Laboratory

    International Nuclear Information System (INIS)

    Wagner, John C.; Peplow, Douglas E.; Mosher, Scott W.; Evans, Thomas M.

    2010-01-01

    This paper provides a review of the hybrid (Monte Carlo/deterministic) radiation transport methods and codes used at the Oak Ridge National Laboratory and examples of their application for increasing the efficiency of real-world, fixed-source Monte Carlo analyses. The two principal hybrid methods are (1) Consistent Adjoint Driven Importance Sampling (CADIS) for optimization of a localized detector (tally) region (e.g., flux, dose, or reaction rate at a particular location) and (2) Forward Weighted CADIS (FW-CADIS) for optimizing distributions (e.g., mesh tallies over all or part of the problem space) or multiple localized detector regions (e.g., simultaneous optimization of two or more localized tally regions). The two methods have been implemented and automated in both the MAVRIC sequence of SCALE 6 and ADVANTG, a code that works with the MCNP code. As implemented, the methods utilize the results of approximate, fast-running 3-D discrete ordinates transport calculations (with the Denovo code) to generate consistent space- and energy-dependent source and transport (weight windows) biasing parameters. These methods and codes have been applied to many relevant and challenging problems, including calculations of PWR ex-core thermal detector response, dose rates throughout an entire PWR facility, site boundary dose from arrays of commercial spent fuel storage casks, radiation fields for criticality accident alarm system placement, and detector response for special nuclear material detection scenarios and nuclear well-logging tools. Substantial computational speed-ups, generally O(102-4), have been realized for all applications to date. This paper provides a brief review of the methods, their implementation, results of their application, and current development activities, as well as a considerable list of references for readers seeking more information about the methods and/or their applications.

  3. Review of hybrid (deterministic/Monte Carlo) radiation transport methods, codes, and applications at Oak Ridge National Laboratory

    International Nuclear Information System (INIS)

    Wagner, J.C.; Peplow, D.E.; Mosher, S.W.; Evans, T.M.

    2010-01-01

    This paper provides a review of the hybrid (Monte Carlo/deterministic) radiation transport methods and codes used at the Oak Ridge National Laboratory and examples of their application for increasing the efficiency of real-world, fixed-source Monte Carlo analyses. The two principal hybrid methods are (1) Consistent Adjoint Driven Importance Sampling (CADIS) for optimization of a localized detector (tally) region (e.g., flux, dose, or reaction rate at a particular location) and (2) Forward Weighted CADIS (FW-CADIS) for optimizing distributions (e.g., mesh tallies over all or part of the problem space) or multiple localized detector regions (e.g., simultaneous optimization of two or more localized tally regions). The two methods have been implemented and automated in both the MAVRIC sequence of SCALE 6 and ADVANTG, a code that works with the MCNP code. As implemented, the methods utilize the results of approximate, fast-running 3-D discrete ordinates transport calculations (with the Denovo code) to generate consistent space- and energy-dependent source and transport (weight windows) biasing parameters. These methods and codes have been applied to many relevant and challenging problems, including calculations of PWR ex-core thermal detector response, dose rates throughout an entire PWR facility, site boundary dose from arrays of commercial spent fuel storage casks, radiation fields for criticality accident alarm system placement, and detector response for special nuclear material detection scenarios and nuclear well-logging tools. Substantial computational speed-ups, generally O(10 2-4 ), have been realized for all applications to date. This paper provides a brief review of the methods, their implementation, results of their application, and current development activities, as well as a considerable list of references for readers seeking more information about the methods and/or their applications. (author)

  4. X-ray sensitivity of somatic cell hybrids

    International Nuclear Information System (INIS)

    Zampetti-Bosseler, F.; Heilporn, V.; Lievens, A.; Limbosch, S.

    1976-01-01

    Different somatic cell hybrids have been studied as a function of their x-ray survival and karyotypic properties. Hybrids between x-ray-sensitive mouse lymphoma cells and mouse fibroblasts, retaining a large proportion of both parental chromosomes, were much more resistant to irradiation than either of the parental cells. On the other hand, hybrids between sensitive mouse lymphoma cells and hamster fibroblasts which also retained a relatively high number of chromosomes from both parents had a sensitivity intermediate between the sensitivities of the parental cell lines. Finally, hybrids between mouse fibroblasts and hamster fibroblasts carrying at least one hamster genome and less than one mouse genome resembled the hamster parent with respect to survival capactity. The significance of these results is discussed

  5. Lightning in hybrid cable-overhead lines and consequent transient overvoltages

    DEFF Research Database (Denmark)

    Silva, Filipe Miguel Faria da; Schultz Pedersen, Kasper; Bak, Claus Leth

    2017-01-01

    Whereas lines that consist entirely of underground cable are naturally protected from lightning, the phenomenon must be considered for lines that are a mix of overhead lines and underground cables. The difference between overhead lines and underground cables wave impedances may lead to transient ...... conductor and for hits on the earth wire, with and without back flashover. For the most common situation, stroke on the earth wire, it is demonstrated that the cable’s ground propagation mode is dominant, reducing the impact of the reflections....

  6. Radiation Calibration Measurements

    International Nuclear Information System (INIS)

    Omondi, C.

    2017-01-01

    KEBS Radiation Dosimetry mandate are: Custodian of Kenya Standards on Ionizing radiation, Ensure traceability to International System (SI ) and Calibration radiation equipment. RAF 8/040 on Radioisotope applications for troubleshooting and optimizing industrial process established Radiotracer Laboratory objective is to introduce and implement radiotracer technique for problem solving of industrial challenges. Gamma ray scanning technique applied is to Locate blockages, Locate liquid in vapor lines, Locate areas of lost refractory or lining in a pipe and Measure flowing densities. Equipment used for diagnostic and radiation protection must be calibrated to ensure Accuracy and Traceability

  7. A high resolution radiation hybrid map of bovine chromosome 14 identifies scaffold rearrangement in the latest bovine assembly

    Directory of Open Access Journals (Sweden)

    Wang Zhiquan

    2007-07-01

    Full Text Available Abstract Background Radiation hybrid (RH maps are considered to be a tool of choice for fine mapping closely linked loci, considering that the resolution of linkage maps is determined by the number of informative meiosis and recombination events which may require very large mapping populations. Accurately defining the marker order on chromosomes is crucial for correct identification of quantitative trait loci (QTL, haplotype map construction and refinement of candidate gene searches. Results A 12 k Radiation hybrid map of bovine chromosome 14 was constructed using 843 single nucleotide polymorphism markers. The resulting map was aligned with the latest version of the bovine assembly (Btau_3.1 as well as other previously published RH maps. The resulting map identified distinct regions on Bovine chromosome 14 where discrepancies between this RH map and the bovine assembly occur. A major region of discrepancy was found near the centromere involving the arrangement and order of the scaffolds from the assembly. The map further confirms previously published conserved synteny blocks with human chromosome 8. As well, it identifies an extra breakpoint and conserved synteny block previously undetected due to lower marker density. This conserved synteny block is in a region where markers between the RH map presented here and the latest sequence assembly are in very good agreement. Conclusion The increase of publicly available markers shifts the rate limiting step from marker discovery to the correct identification of their order for further use by the research community. This high resolution map of bovine chromosome 14 will facilitate identification of regions in the sequence assembly where additional information is required to resolve marker ordering.

  8. Innovative hybrid biological reactors using membranes

    International Nuclear Information System (INIS)

    Diez, R.; Esteban-Garcia, A. L.; Florio, L. de; Rodriguez-Hernandez, L.; Tejero, I.

    2011-01-01

    In this paper we present two lines of research on hybrid reactors including the use of membranes, although with different functions: RBPM, biofilm reactors and membranes filtration RBSOM, supported biofilm reactors and oxygen membranes. (Author) 14 refs.

  9. The integrin α6β4 as a signaling membrane protein for a damage response to ionizing radiation in human prostate cancer cell lines

    International Nuclear Information System (INIS)

    Woo, Charles; Nagle, Ray B.; Stea, Baldassarre; Cress, Anne E.

    1996-01-01

    Purpose/Object: Integrins are cell surface receptors that exist as heterodimers. The integrin α6β4 is a receptor for laminin and is present in normal human prostate tissue. In prostate carcinoma however, there is loss of β4 expression. Prior studies demonstrated that when a low β4 expressing rectal carcinoma cell line was transfected with β4, the cells underwent apoptosis. We investigated the effects that the β4 integrin had on DNA damage responses in a human prostate carcinoma line. Materials and Methods: DU-145 human prostate carcinoma cells previously selected by us for α6β1 expression were transfected with either a full length β4 construct or vector only. Both cell lines were grown simultaneously and maintained in geneticin for selection purposes. Cells were grown on glass coverslips in 60mm tissue culture dishes under optimal growth conditions. Radiation was delivered using a Co-60 machine with a dose rate of 35 Gy/hr. The cells were given 0, 2, 5, and 10 Gy. Three different radiation damage responses were assayed and include micronuclei (MN) formation, cell cycle distribution, and cell survival. 24 hours after irradiation, the cells were fixed and stained with propidium iodide. Micronuclei formation was detected using a Zeiss LSM10 confocal microscope, and the resulting digital images were analyzed using the NIH Image program. The observed MN were detected without the use of cytochalasin B, but were noted to contain nuclear histone and DNA and were morphologically distinct from apoptotic or necrotic bodies. Results: The quantitative analysis of MN formation revealed a radiation dose dependence of MN formation in both the α6β4 and α6β1 expressing cell lines. The presence of MN 24 hours after irradiation was observed at clinically significant doses (2 Gy) with the largest effect occurring at 5 Gy. The α6β4 expressing cells consistently produced approximately two fold more MN as compared to the α6β1 expressing cells at all radiation doses. The

  10. Patterns of cytosine methylation in an elite rice hybrid and its parental lines, detected by a methylation-sensitive amplification polymorphism technique.

    Science.gov (United States)

    Xiong, L Z; Xu, C G; Saghai Maroof, M A; Zhang, Q

    1999-04-01

    DNA methylation is known to play an important role in the regulation of gene expression in eukaryotes. In this study, we assessed the extent and pattern of cytosine methylation in the rice genome, using the technique of methylation-sensitive amplified polymorphism (MSAP), which is a modification of the amplified fragment length polymorphism (AFLP) method that makes use of the differential sensitivity of a pair of isoschizomers to cytosine methylation. The tissues assayed included seedlings and flag leaves of an elite rice hybrid, Shanyou 63, and the parental lines Zhenshan 97 and Minghui 63. In all, 1076 fragments, each representing a recognition site cleaved by either or both of the isoschizomers, were amplified using 16 pairs of selective primers. A total of 195 sites were found to be methylated at cytosines in one or both parents, and the two parents showed approximately the same overall degree of methylation (16.3%), as revealed by the incidence of differential digestion by the isoschizomers. Four classes of patterns were identified in a comparative assay of cytosine methylation in the parents and hybrid; increased methylation was detected in the hybrid compared to the parents at some of the recognition sites, while decreased methylation in the hybrid was detected at other sites. A small proportion of the sites was found to be differentially methylated in seedlings and flag leaves; DNA from young seedlings was methylated to a greater extent than that from flag leaves. Almost all of the methylation patterns detected by MSAP could be confirmed by Southern analysis using the isolated amplified fragments as probes. The results clearly demonstrate that the MSAP technique is highly efficient for large-scale detection of cytosine methylation in the rice genome. We believe that the technique can be adapted for use in other plant species.

  11. Signal integrity analysis on discontinuous microstrip line

    International Nuclear Information System (INIS)

    Qiao, Qingyang; Dai, Yawen; Chen, Zipeng

    2013-01-01

    In high speed PCB design, microstirp lines were used to control the impedance, however, the discontinuous microstrip line can cause signal integrity problems. In this paper, we use the transmission line theory to study the characteristics of microstrip lines. Research results indicate that the discontinuity such as truncation, gap and size change result in the problems such as radiation, reflection, delay and ground bounce. We change the discontinuities to distributed parameter circuits, analysed the steady-state response and transient response and the phase delay. The transient response cause radiation and voltage jump.

  12. DEVELOPMENT OF MELON F1 SEEDS BASED ON LINES WITH GENIC MALE STERILITY

    Directory of Open Access Journals (Sweden)

    A. S. Sokolov

    2014-01-01

    Full Text Available The perspective technology of development of melon of F1hybrids seeds by use maternal lines with an original form of genic mail sterility and marker trait (lobed leaves was studied. Elements of technology allow developing hybrid seeds of melon with hybridity of 90-95%.

  13. Application of the spine-layer jet radiation model to outbursts in the broad-line radio galaxy 3C 120

    Science.gov (United States)

    Janiak, M.; Sikora, M.; Moderski, R.

    2016-05-01

    We present a detailed Fermi/LAT data analysis for the broad-line radio galaxy 3C 120. This source has recently entered into a state of increased γ-ray activity which manifested itself in two major flares detected by Fermi/LAT in 2014 September and 2015 April with no significant flux changes reported in other wavelengths. We analyse available data focusing our attention on aforementioned outbursts. We find very fast variability time-scale during flares (of the order of hours) together with a significant γ-ray flux increase. We show that the ˜6.8 yr averaged γ-ray emission of 3C 120 is likely a sum of the external radiation Compton and the synchrotron self-Compton radiative components. To address the problem of violent γ-ray flares and fast variability we model the jet radiation dividing the jet structure into two components: the wide and relatively slow outer layer and the fast, narrow spine. We show that with the addition of the fast spine occasionally bent towards the observer we are able to explain observed spectral energy distribution of 3C 120 during flares with the Compton upscattered broad-line region and dusty torus photons as main γ-rays emission mechanism.

  14. A New Radio Spectral Line Survey of Planetary Nebulae: Exploring Radiatively-driven Heating and Chemistry of Molecular Gas

    Science.gov (United States)

    Bublitz, Jesse; Kastner, Joel H.; Santander-García, Miguel; Montez, Rodolfo; Alcolea, Javier; Balick, Bruce; Bujarrabal, Valentín

    2018-01-01

    We report the results of a survey of mm-wave molecular line emission from nine nearby (Radioastronomie Millimétrique (IRAM) 30 m telescope. Our sample comprises molecule-rich PNe spanning a wide range of central star UV luminosities as well as central star and nebular X-ray emission properties. Nine molecular line frequencies were chosen to investigate the molecular chemistry of these nebulae. New detections of one or more of five molecules -- the molecular mass tracer 13CO and the chemically important trace species HCO+, CN, HCN, and HNC -- were made in at least one PN. We present analysis of emission line flux ratios that are potential diagnostics of the influence that ultraviolet and X-ray radiation have on the chemistry of residual molecular gas in PNe.

  15. PRODUCTIVITY OF SUGAR BEET LINES AND THEIR CROSSES DEPENDING ON PLOIDITY

    Directory of Open Access Journals (Sweden)

    Andrija Kristek

    2003-12-01

    Full Text Available Five diploid (2n=2x=18 cms lines, 2 tetraploid (2n=4x=36 and 2 diploid pollinators, as well as 10 diploid and 10 triploid (2n=3x=27 sugar beet hybrids, given by the crossing of investigated cms lines and pollinators were investigated in the field trials. Two triploid hybrids, widespread in sugarbeet production, were sown as standards – Os Sana and Iva. The trials were conducted on two localities (Osijek and Đakovo during the two years (2002 and 2003. There was a difference between years in weather conditions and between localities in terms of type and features of soil. First year of the investigation was humid and warm and the second was dry and hot. Osijek locality was characterized by chernozem-meadow type soil and Đakovo by loessial pseudoglei. The best average root yield was achieved between the investigated genotypes by the triploid hybrid 15 (58.09 t/ha and the hybrids 11, 13 and standard 31. As for the content and utilization of sugar, the standard 31 achieved best results (15.15% followed by the standard hybrids 15, 18, 17 and 11. The best sugar yield was achieved by hybrid 15 (7.08 t/ha, followed by hybrids 13, 11, 10 and 18.

  16. SU-E-T-13: A Feasibility Study of the Use of Hybrid Computational Phantoms for Improved Historical Dose Reconstruction in the Study of Late Radiation Effects for Hodgkin's Lymphoma

    Energy Technology Data Exchange (ETDEWEB)

    Petroccia, H; O' Reilly, S; Bolch, W [J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL (United States); Mendenhall, N; Li, Z; Slopsema, R [Radiation Oncology, University of Florida Proton Therapy Institute, Jacksonville, FL (United States)

    2014-06-01

    Purpose: Radiation-induced cancer effects are well-documented following radiotherapy. Further investigation is needed to more accurately determine a dose-response relationship for late radiation effects. Recent dosimetry studies tend to use representative patients (Taylor 2009) or anthropomorphic phantoms (Wirth 2008) for estimating organ mean doses. In this study, we compare hybrid computational phantoms to patient-specific voxel phantoms to test the accuracy of University of Florida Hybrid Phantom Library (UFHP Library) for historical dose reconstructions. Methods: A cohort of 10 patients with CT images was used to reproduce the data that was collected historically for Hodgkin's lymphoma patients (i.e. caliper measurements and photographs). Four types of phantoms were generated to show a range of refinement from reference hybrid-computational phantom to patient-specific phantoms. Each patient is matched to a reference phantom from the UFHP Library based on height and weight. The reference phantom is refined in the anterior/posterior direction to create a ‘caliper-scaled phantom’. A photograph is simulated using a surface rendering from segmented CT images. Further refinement in the lateral direction is performed using ratios from a simulated-photograph to create a ‘photograph and caliper-scaled phantom’; breast size and position is visually adjusted. Patient-specific hybrid phantoms, with matched organ volumes, are generated and show the capabilities of the UF Hybrid Phantom Library. Reference, caliper-scaled, photograph and caliper-scaled, and patient-specific hybrid phantoms are compared with patient-specific voxel phantoms to determine the accuracy of the study. Results: Progression from reference phantom to patient specific hybrid shows good agreement with the patient specific voxel phantoms. Each stage of refinement shows an overall trend of improvement in dose accuracy within the study, which suggests that computational phantoms can show

  17. A putative hybrid swarm within Oonopsis foliosa (Asteraceae: Astereae)

    Science.gov (United States)

    Hughes, J.F.; Brown, G.K.

    2004-01-01

    Oo??nopsis foliosa var. foliosa and var. monocephala are endemic to short-grass steppe of southeastern Colorado and until recently were considered geographically disjunct. The only known qualitative feature separating these 2 varieties is floral head type; var. foliosa has radiate heads, whereas var. monocephala heads are discoid. Sympatry between these varieties is restricted to a small area in which a range of parental types and intermediate head morphologies is observed. We used distribution mapping, morphometric analyses, chromosome cytology, and pollen stainability to characterize the sympatric zone. Morphometrics confirms that the only discrete difference between var. foliosa and var. monocephala is radiate versus discoid heads, respectively. The outer florets of putative hybrid individuals ranged from conspicuously elongated yet radially symmetric disc-floret corollas, to elongated radially asymmetric bilabiate- or deeply cleft corollas, to stunted ray florets with appendages remnant of corolla lobes. Chromosome cytology of pollen mother cells from both putative parental varieties and a series of intermediate morphological types collected at the sympatric zone reveal evidence of translocation heterozygosity. Pollen stainability shows no significant differences in viability between the parental varieties and putative hybrids. The restricted distribution of putative hybrids to a narrow zone of sympatry between the parental types and the presence of meiotic chromosome-pairing anomalies in these intermediate plants are consistent with a hybrid origin. The high stainability of putative-hybrid pollen adds to a growing body of evidence that hybrids are not universally unfit.

  18. Hybrid vigour and gene action for two quantitative traits of castor ...

    African Journals Online (AJOL)

    Five homozygous lines of castor plant, namely RS1-Om, RN1-Om, RTl-2m, RSl- Owm, and RNl- Omb, were crossed to raise F1, F2, BC1, and BC2 generations. The hybrids were tested for hybrid vigour for two metric traits, viz; number of pods per plant and seed yield per plant. Highly significant hybrid vigour was detected ...

  19. Agronomic characteristic of a dwarf germplasm sunflower line

    OpenAIRE

    Vassilevska-Ivanova Roumiana; Tcekova Zoja

    2005-01-01

    A new sunflower (Helianthus annuus L) dwarf line, HA-ARG-1, has been developed after interspecific hybridization between cultivated sunflower H. annuus and wild annual silver-leaf sunflower H. argophyllus. Plants were selected for reduced height and multiple branching characteristics. The agronomic, morphologic and oil content characteristics of the line were analyzed. Isolation of similar dwarfed lines illustrates the importance of using the wild sunflower germplasms in the development of sp...

  20. What is radiation curing

    International Nuclear Information System (INIS)

    Kinstle, J.F.

    1975-01-01

    Radiation curing is a highly interdisciplinary and sophisticated field. Successful interplay between chemists and engineers of various disciplines is required. Throughout the research-development-applications cycle, two disciplines for which hybridization is extremely important are radiation chemistry and polymer chemistry. The molecular level effects caused by absorbed radiation depend strongly on the type and intensity of the radiation. Efficient utilization of the radiation to effect desired transformations in a monomer and/or polymer system, and maximization of final properties, depend on well-planned polymer synthesis and system formulation. The elementary basis of these two disciplines and the manner in which they necessarily coalesce in the field of radiation curing are reviewed

  1. Beam transport radiation shielding for branch lines 2-ID-B and 2-ID-C

    International Nuclear Information System (INIS)

    Feng, Y.P.; Lai, B.; McNulty, I.; Dejus, R.J.; Randall, K.J.; Yun, W.

    1995-01-01

    The x-ray radiation shielding requirements beyond the first optics enclosure have been considered for the beam transport of the 2-ID-B and 2-ID-C branch lines of Sector 2 (SRI-CAT) of the APS. The first three optical components (mirrors) of the 2-ID-B branch are contained within the shielded first optics enclosure. Calculations indicate that scattering of the primary synchrotron beam by beamline components outside the enclosure, such as apertures and monochromators, or by gas particles in case of vacuum failure is within safe limits for this branch. A standard 2.5-inch-diameter stainless steel pipe with 1/16-inch-thick walls provides adequate shielding to reduce the radiation dose equivalent rate to human tissue to below the maximum permissible limit of 0.25 mrem/hr. The 2-ID-C branch requires, between the first optics enclosure where only two mirrors are used and the housing for the third mirror, additional lead shielding (0.75 mm) and a minimum approach distance of 2.6 cm. A direct beam stop consisting of at least 4.5 mm of lead is also required immediately downstream of the third mirror for 2-ID-C. Finally, to stop the direct beam from escaping the experimental station, a beam stop consisting of at least 4-mm or 2.5-mm steel is required for the 2-ID-B or 2-ID-C branches, respectively. This final requirement can be met by the vacuum chambers used to house the experiments for both branch lines

  2. Modulation of interferon-gamma-induced HLA-DR expression on the human keratinocyte cell line SCC-13 by ultraviolet radiation

    International Nuclear Information System (INIS)

    Khan, I.U.; Boehm, K.D.; Elmets, C.A.

    1993-01-01

    Cell surface expression of major histocompatibility determinants on epidermal keratinocytes is a characteristic feature of a number of inflammatory dermatoses and in all likelihood is caused by diffusion of human leukocyte antigen (HLA)-DR-inducing cytokines from cells present in the dermal mononuclear cell infiltrate. Many of these same disorders respond to ultraviolet (UV) radiation phototherapy. Using the human SCC-13 keratinocyte cell line as a model, UV radiation was found to inhibit interferon-gamma-induced HLA-DR expression. Inhibition correlated closely with decreased steady-state levels of HLA-DR mRNA. These findings provide evidence that the therapeutic effect of UV radiation phototherapy may be mediated by its capacity to down-regulate cytokine-induced keratinocyte HLA-DR expression. (Author)

  3. Electric-line-source illumination of a circular cylinder of lossless double-negative material: an investigation of near field, directivity, and radiation resistance

    DEFF Research Database (Denmark)

    Arslanagic, Samel; Breinbjerg, Olav

    2006-01-01

    the properties of the near field, inside as well as outside the cylinder, and the far-field. Third, the variations of these fields are examined, as well as the radiation resistance and radiation pattern, as functions of the geometrical and electromagnetic parameters of the configuration. It is demonstrated......This work investigates the properties of an antenna-like configuration with an electric line source radiating in the presence of a double-negative circular cylinder. First, the analytical eigenfunction-series solution is derived. Second, this solution is employed in numerical calculations to study...

  4. The possibility of developing hybrid PV/T solar system

    Science.gov (United States)

    Dobrnjac, M.; Zivkovic, P.; Babic, V.

    2017-05-01

    An alternative and cost-effective solution to developing integrated PV system is to use hybrid photovoltaic/thermal (PV/T) solar system. The temperature of PV modules increases due to the absorbed solar radiation that is not converted into electricity, causing a decrease in their efficiency. In hybrid PV/T solar systems the reduction of PV module temperature can be combined with a useful fluid heating. In this paper we present the possibility of developing a new hybrid PV/T solar system. Hybrid PV/T system can provide electrical and thermal energy, thus achieving a higher energy conversion rate of the absorbed solar radiation. We developed PV/T prototype consisted of commercial PV module and thermal panel with our original solution of aluminium absorber with special geometric shapes. The main advantages of our combined PV/T system are: removing of heat from the PV panel; extending the lifetime of photovoltaic cells; excess of the removing heat from PV part is used to heat the fluid in the thermal part of the panel; the possibility of using on the roof and facade constructions because less weight.

  5. Hybrid Active-Passive Radiation Shielding System

    Data.gov (United States)

    National Aeronautics and Space Administration — A radiation shielding system is proposed that integrates active magnetic fields with passive shielding materials. The objective is to increase the shielding...

  6. Some Results On The Modelling Of TSS Manufacturing Lines

    Directory of Open Access Journals (Sweden)

    Viorel MÎNZU

    2000-12-01

    Full Text Available This paper deals with the modelling of a particular class of manufacturing lines, governed by a decentralised control strategy so that they balance themselves. Such lines are known as “bucket brigades” and also as “TSS lines”, after their first implementation, at Toyota, in the 70’s. A first study of their behaviour was based upon modelling as stochastic dynamic systems, which emphasised, in the frame of the so-called “Normative Model”, a sufficient condition for self-balancing, that means for autonomous functioning at a steady production rate (stationary behaviour. Under some particular conditions, a simulation analysis of TSS lines could be made on non-linear block diagrams, showing that the state trajectories are piecewise continuous in between occurrences of certain discrete events, which determine their discontinuity. TSS lines may therefore be modelled as hybrid dynamic systems, more specific, with autonomous switching and autonomous impulses (jumps. A stability analysis of such manufacturing lines is allowed by modelling them as hybrid dynamic systems with discontinuous motions.

  7. Suppression subtractive hybridization profiles of radial growth phase and metastatic melanoma cell lines reveal novel potential targets

    International Nuclear Information System (INIS)

    Sousa, Josane F; Espreafico, Enilza M

    2008-01-01

    Melanoma progression occurs through three major stages: radial growth phase (RGP), confined to the epidermis; vertical growth phase (VGP), when the tumor has invaded into the dermis; and metastasis. In this work, we used suppression subtractive hybridization (SSH) to investigate the molecular signature of melanoma progression, by comparing a group of metastatic cell lines with an RGP-like cell line showing characteristics of early neoplastic lesions including expression of the metastasis suppressor KISS1, lack of αvβ3-integrin and low levels of RHOC. Two subtracted cDNA collections were obtained, one (RGP library) by subtracting the RGP cell line (WM1552C) cDNA from a cDNA pool from four metastatic cell lines (WM9, WM852, 1205Lu and WM1617), and the other (Met library) by the reverse subtraction. Clones were sequenced and annotated, and expression validation was done by Northern blot and RT-PCR. Gene Ontology annotation and searches in large-scale melanoma expression studies were done for the genes identified. We identified 367 clones from the RGP library and 386 from the Met library, of which 351 and 368, respectively, match human mRNA sequences, representing 288 and 217 annotated genes. We confirmed the differential expression of all genes selected for validation. In the Met library, we found an enrichment of genes in the growth factors/receptor, adhesion and motility categories whereas in the RGP library, enriched categories were nucleotide biosynthesis, DNA packing/repair, and macromolecular/vesicular trafficking. Interestingly, 19% of the genes from the RGP library map to chromosome 1 against 4% of the ones from Met library. This study identifies two populations of genes differentially expressed between melanoma cell lines from two tumor stages and suggests that these sets of genes represent profiles of less aggressive versus metastatic melanomas. A search for expression profiles of melanoma in available expression study databases allowed us to point to a

  8. Determination of gamma radiation lethal dose (LD50) and resveratrol cytotoxicity level in tumor cells line

    International Nuclear Information System (INIS)

    Magalhaes, Vanessa D.; Rogero, Sizue O.; Rogero, Jose R.; Cruz, Aurea S.

    2011-01-01

    Cancer is a disease with high incidence and it is considered a worldwide public health problem. Resveratrol is a polyphenol occurring naturally in a wide variety of plants according to response of ultraviolet radiation (UV) exposition or according to mechanical stress resulting of pathogens or chemical and physical agents. This polyphenol possesses a pharmacological activity of carcinogenesis inhibition in multiple levels. It also protects cells by scavenging the free radicals which are considered toxic products. These free radicals are formed of natural process of cell aging and also by incidence of ionizing radiation in the organism. Thus, resveratrol is considered as a cell radioprotector. On the other hand, in some elevated concentrations resveratrol may be considered as a radiosensitizing. The aim of this work was the determination of radiation lethal dose (LD 50 ) and also verifies the cytotoxicity level of resveratrol in tumor cells line: muco epidermoid pulmonary carcinoma cells (NCI-H292) and rhabdomyosarcoma cells (RD). The cytotoxicity test was performed by neutral red uptake assay. The results of resveratrol IC 50% in NCI-H292 cells was 192μM and in RD cells was 128μM; and RD cells gamma radiation LD 50 was 435Gy. (author)

  9. Derivation of hybrid ES cell lines from two different strains of mice

    Directory of Open Access Journals (Sweden)

    Ho-Tak Lau

    2016-03-01

    Full Text Available Parental origin-dependent expression of the imprinted genes is essential for mammalian development. Zfp57 maintains genomic imprinting in mouse embryos and ES cells. To examine the allelic expression patterns of the imprinted genes in ES cells, we obtained multiple hybrid ES clones that were directly derived from the blastocysts generated from the cross between mice on two different genetic backgrounds. The blastocyst-derived ES clones displayed largely intact DNA methylation imprint at the tested imprinted regions. These hybrid ES clones will be useful for future studies to examine the allelic expression of the imprinted genes in ES cells and their differentiated progeny.

  10. Remote inflation as hybrid-like sneutrino/MSSM inflation

    International Nuclear Information System (INIS)

    Matsuda, Tomohiro

    2009-01-01

    A new scenario of hybrid-like inflation is considered for sneutrino and MSSM fields. Contrary to the usual hybrid inflation model, the direct coupling between a trigger field and the sneutrino/MSSM inflaton field is not necessary for the scenario. The dissipation and the radiation from the sneutrino/MSSM inflaton can be written explicitly by using the Yukawa couplings. Remote inflation does not require the shift symmetry or cancellation in solving the η problem.

  11. Further studies on the possible relationship between radiation-induced reciprocal translocations and intrinsic radiosensitivity of human tumor cells

    International Nuclear Information System (INIS)

    Virsik-Peuckert, P.; Rave-Fraenk, M.; Schmidberger, H.

    1996-01-01

    Background and purpose. The aim of the present study was to estimate yields of radiation-induced translocations in surviving cells of several human tumor cell lines and in normal diploid human fibroblasts, and to compare these yields with corresponding intrinsic radiosensitivities determined by standard colony-formation assay. Material and methods. The yields of radiation-induced reciprocal translocations were investigated by fluorescence in situ hybridization. Chromosomes no. 1 and no. 4 were 'painted' with fluorescent hybridization probes for whole chromosomes. Translocation yields and cell survival were determined for different doses up to 6 Gy of 200 kV X-rays. Results. We observed a higher frequency of reciprocal translocations in the radiosensitive cells MCF-7 and MDA-MB-436 than in the radioresistant cells CaSki, WiDr, A549 and normal skin fibroblasts. For primary squamous cell carcinoma cells, ZMK-1, an intermediate radiosensitivity and an intermediate translocation yield were observed. The dose-dependence of translocation yields involving chromosomes no. 1 or no. 4 varied in different cell lines: it was linear or linear with a plateau at higher doses. Conclusions. A comparison of the data obtained with chromosomes no. 1 and no. 4 in the investigated cell types, indicates that intrinsic radiosensitivity of different tumor cells observed at the survival level, is correlated with different translocation yields, respectively. This correlation was observed for all cell types investigated, independent of the number of copies of the painted chromosome per cell or the radiation dose. However, for low doses (under 1 Gy), the yields of translocations determined for the individual chromosomes seem to be too low for a discrimination between radioresistant or radiosensitive cells

  12. Characterization of the binding of radioiodinated hybrid recombinant IFN-alpha A/D to murine and human lymphoid cell lines

    International Nuclear Information System (INIS)

    Faltynek, C.R.; Princler, G.L.; Schwabe, M.; Shata, M.T.; Lewis, G.K.; Kamin-Lewis, R.M.

    1990-01-01

    The hybrid recombinant human interferon (IFN) rIFN-alpha A/D was radioiodinated. Specific binding of [125I]rIFN-alpha A/D was observed with both human and murine cell lines. The binding of [125I]rIFN-alpha A/D to human Daudi cells had similar characteristics to the previously described binding of [125I]rIFN-alpha A or -alpha 2. The following lines of evidence demonstrated that [125I]rIFN-alpha A/D bound with high affinity to the same receptor on murine cells as murine IFN-alpha and -beta: (i) the binding of [125I]rIFN-alpha A/D to murine LBRM cells was inhibited to a similar extent by natural murine IFN-alpha, natural murine IFN-beta, and rIFN-A/D; (ii) the Kd (approximately 2 X 10(-10) M) obtained from both competition experiments and saturation binding experiments with [125I]rIFN-alpha A/D was comparable to the previously reported Kd for the binding of natural murine IFN-alpha and -beta to other murine cell lines; (iii) the size of the cross-linked [125I]rIFN-alpha A/D receptor complex formed on murine LBRM cells was similar to the previously reported cross-linked complex formed after binding radioiodinated natural murine IFN-beta to other murine cell lines. Due to the current lack of readily available recombinant murine IFN-alpha or -beta for radiolabeling and the previously demonstrated biological activity of rIFN-alpha A/D on murine cells, [125I]rIFN-alpha A/D should prove to be a useful reagent for further studies of murine IFN receptors

  13. Influence of Genetic Drift of Restoring Gene (Rf on Seed Purity of Yuetai A, a Honglian-Type Cytoplasmic Male Sterile Line in Hybrid Rice

    Directory of Open Access Journals (Sweden)

    Ji-feng Wang

    2008-06-01

    Full Text Available The seed samples of Yuetai A, a Honglian (HL type cytoplasmic male sterile (CMS line in hybrid rice were investigated to assess the seed purity and to analyze the cause of off-type plants by imitating the biological contaminant to Yuetai A in Nanjing, Jiangsu Province and Lingshui, Hainan Province during 2004–2006. The seed impurity of Yuetai A mainly resulted from the genetic drift of restoring fertility gene (Rf after biological contamination but not from its sterility unstability. All of the ten maintainer lines, five restorer lines and three thermo-sensitive genic male sterile lines used in the study could pollinate Yuetai A and Yuetai B to produce F1 plants, directly or indirectly resulting in Rf-gene drifting into Yuetai A and generating ‘iso-cytoplasm restoring-generations’. Furthermore, high outcrossing rate and similar heading date of Yuetai A with many varieties used in rice production might easily result in the biological contamination. After removing all plants with Rf-gene mixed in Yuetai A and preventing Rf-gene drifting into Yuetai A, the seed purity of Yuetai A and Yuetai B had been raised to 100%.

  14. Genomic Prediction of Barley Hybrid Performance

    Directory of Open Access Journals (Sweden)

    Norman Philipp

    2016-07-01

    Full Text Available Hybrid breeding in barley ( L. offers great opportunities to accelerate the rate of genetic improvement and to boost yield stability. A crucial requirement consists of the efficient selection of superior hybrid combinations. We used comprehensive phenotypic and genomic data from a commercial breeding program with the goal of examining the potential to predict the hybrid performances. The phenotypic data were comprised of replicated grain yield trials for 385 two-way and 408 three-way hybrids evaluated in up to 47 environments. The parental lines were genotyped using a 3k single nucleotide polymorphism (SNP array based on an Illumina Infinium assay. We implemented ridge regression best linear unbiased prediction modeling for additive and dominance effects and evaluated the prediction ability using five-fold cross validations. The prediction ability of hybrid performances based on general combining ability (GCA effects was moderate, amounting to 0.56 and 0.48 for two- and three-way hybrids, respectively. The potential of GCA-based hybrid prediction requires that both parental components have been evaluated in a hybrid background. This is not necessary for genomic prediction for which we also observed moderate cross-validated prediction abilities of 0.51 and 0.58 for two- and three-way hybrids, respectively. This exemplifies the potential of genomic prediction in hybrid barley. Interestingly, prediction ability using the two-way hybrids as training population and the three-way hybrids as test population or vice versa was low, presumably, because of the different genetic makeup of the parental source populations. Consequently, further research is needed to optimize genomic prediction approaches combining different source populations in barley.

  15. Construction of a line for radiation cross-linking of cable insulators at the Kablo Vrchlabi plant

    International Nuclear Information System (INIS)

    Kourim, P.; Zidek, L.; Kopecky, B.

    1983-01-01

    In order to introduce radiation curing into the technology of manufacturing conductors with silicone insulation the suitability was tested of the individual brands of silicone rubber used for cables. The criterion was the range of doses guaranteeing the required mechanical and rheological properties of the rubber. The ELV 1 accelerator was used for irradiation. A description is given of the design of the technological equipment of the line which was put into experimental operation. (J.P.)

  16. Radiation induced asymmetries in mitotic recombination: evidence for a directional bias in the formation of asymmetric hybrid DNA in yeast

    International Nuclear Information System (INIS)

    Friedman, L.R.; Sobell, H.M.

    We have examined radiation-induced mitotic recombination using two alleles (his1-36, his1-49) in the his1 gene. When the haploid containing his1-36 is irradiated with varying doses of γ rays and then mated with the unirradiated strain containing his1-49, analyses of the selected prototrophs show them to be primarily + +/+ 49. If, on the other hand, the haploid strain containing his1-49 is the irradiated parent, the prototrophic diploids are primarily + +/36 +. In control experiments, where either both strains are irradiated or not irradiated, no such asymmetries are found. These data indicate that the irradiated haploid chromosome tends to be the recipient of genetic information. We interpret these results as indicating a directional bias in the formation of hybrid DNA in radiation-induced mitotic recombination, and discuss these results in terms of current models of genetic recombination

  17. A conjugate gradient method for solving the non-LTE line radiation transfer problem

    Science.gov (United States)

    Paletou, F.; Anterrieu, E.

    2009-12-01

    This study concerns the fast and accurate solution of the line radiation transfer problem, under non-LTE conditions. We propose and evaluate an alternative iterative scheme to the classical ALI-Jacobi method, and to the more recently proposed Gauss-Seidel and successive over-relaxation (GS/SOR) schemes. Our study is indeed based on applying a preconditioned bi-conjugate gradient method (BiCG-P). Standard tests, in 1D plane parallel geometry and in the frame of the two-level atom model with monochromatic scattering are discussed. Rates of convergence between the previously mentioned iterative schemes are compared, as are their respective timing properties. The smoothing capability of the BiCG-P method is also demonstrated.

  18. Fabrication and performance analysis of concentrated hybrid photovoltaic system

    Directory of Open Access Journals (Sweden)

    Murthy Krishna

    2018-01-01

    Full Text Available Sun is the most important source of renewable source of energy. During the past few decades there has been an ever-increasing interest in Photovoltaic (PV cells as it directly converts solar radiation into electricity. This paper involves the performance study of photovoltaic system under concentrated solar radiation. The main problem with the concentration solar energy is the drastic increase in temperature of the photovoltaic module resulting in a decrease in performance efficiency of the system. This problem of overheating of the system can be overcome by providing cooling which would ensure operation of the module in the optimal temperature range. Hence, the setup would function as a hybrid model serving the dual purpose of power generation while also utilizing the waste heat for water heating applications. The experimental set up consist of a novel arrangement of concentrator and reflector and the cooling system. The Hybrid Photovoltaic System was repeatedly tested under real time conditions on several days. A comparison was drawn between the results obtained from direct exposure of a standard photovoltaic module to that obtained from the hybrid system in order to better understand the improvement in performance parameters. The study shown a significant improvement of output of standard photovoltaic module under the concentrated solar radiation.

  19. HTTR plant dynamic simulation using a hybrid computer

    International Nuclear Information System (INIS)

    Shimazaki, Junya; Suzuki, Katsuo; Nabeshima, Kunihiko; Watanabe, Koichi; Shinohara, Yoshikuni; Nakagawa, Shigeaki.

    1990-01-01

    A plant dynamic simulation of High-Temperature Engineering Test Reactor has been made using a new-type hybrid computer. This report describes a dynamic simulation model of HTTR, a hybrid simulation method for SIMSTAR and some results obtained from dynamics analysis of HTTR simulation. It concludes that the hybrid plant simulation is useful for on-line simulation on account of its capability of computation at high speed, compared with that of all digital computer simulation. With sufficient accuracy, 40 times faster computation than real time was reached only by changing an analog time scale for HTTR simulation. (author)

  20. Diagnostics of red-shifted H-alpha line emission from a C-class flare with full non-LTE radiative and hydrodynamic approach

    Science.gov (United States)

    Druett, M. K.; Zharkova, V. V.; Scullion, E.; Zharkov, S.; Matthews, S. A.

    2016-12-01

    We analyse H-alpha line profiles with strong redshifts during the C1.8 flare on 1st July 2012 obtained from the Swedish Solar Telescope (SST) closely resembling the previous observations (Wuelser and Marti, 1989). The flare has a magnetic field configuration with two levels of loop structures. The kernels with red shifts are observed in one of the H-alpha ribbons in the south-west location formed after the main impulse recorded in the north-east. The locations of H-alpha kernels with red shifts reveal close temporal and spatial correlation with weaker HXR signatures and coincide with the locations of coronal jets observed with AIA/SDO. For interpretation we apply a revised 1D hydrodynamic and non-LTE (NLTE) radiative model for 5 level plus continuum model hydrogen atom (Druett & Zharkova, 2016) considering radiative, thermal and non-thermal excitation and ionisation by beam electrons with the updated beam densities (Zharkova & Dobranskis, 2016) and analytical excitation/ionisation rates (Zharkova& Kobylinskijj, 1993). We find the simultaneous solutions of steady state and radiative transfer equations in all optically-thick lines and continua. The electron and ion temperatures, ambient density and macrovelocity of the ambient plasma are derived from a 1D hydrodynamic model with initial condition of the pre-flaring photosphere for the two fluid ambient plasma heated by beam electrons (Zharkova & Zharkov, 2007). We simulate distributions over precipitation depth of ionisation and departure coefficients for all the hydrogen atom transitions including the deviation of ionisation from Saha equation affected by non-thermal electron beams. We show that in the very first seconds after the beam onset Balmer line profiles are sensitive to the effect of beam electrons. The combination of the additional ionisation caused by beam electrons leading to a very strong Stark effect in Balmer lines with the hydrodynamic heating and formation of a low temperature shock in the

  1. Study of an hybrid positron source using channeling for CLIC

    CERN Document Server

    Dadoun, O; Chehab, R; Poirier, F; Rinolfi, L; Strakhovenko, V; Variola, A; Vivoli, A

    2009-01-01

    The CLIC study considers the hybrid source using channeling as the baseline for positron production. The hybrid source uses a few GeV electron beam impinging on a crystal tungsten radiator. With the tungsten crystal oriented on its axis it results an intense, relatively low energy photon beam due mainly to channeling radiation. Those photons are then impinging on an amorphous tungsten target producing positrons by e+e− pair creation. In this note the optimization of the positron yield and the peak energy deposition density in the amorphous target are studied according to the distance between the crystal and the amorphous targets, the primary electron energy and the amorphous target thickness.

  2. Pharmacological manipulation of radiation induced apoptosis in a cervical carcinoma cell line

    International Nuclear Information System (INIS)

    Kamradt, M.; Mohideen, N.; Krueger, E.; Sokolova, I.A.; Khodarev, N.N; Vaughan, A.T.M.

    1997-01-01

    Purpose: Radiotherapy is a curative option in the treatment of early stage cervical carcinoma and radioresistant tumors limit local control and survival. Therefore, it is important to understand mechanisms by which cells evade death after irradiation. Of these, the process of apoptosis offers a useful model system to study. Tumors of the cervix offer a unique opportunity in that most contain an HPV genome expressing the E6 and E7 gene products under the control of a glucocorticoid responsive promoter. The HPV E6 and E7 proteins target p53 and/or Rb, both of which are involved in cell cycle control and in the apoptotic process. It was previously determined that treatment with the corticosteroid dexamethasone increased transcription of E6 and E7 and