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Sample records for radiacoes gamma 60co

  1. Effect of gamma radiation of {sup 60}Co in the conservation of seeds and on the productivity of bean plant (Phaseolus vulgaris L.).; Efeitos de radiacoes gama do {sup 60}Co na conservacao da semente e na produtividade do feijoeiro (Phaseolus vulgaris L.)

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    Marcos Filho, Julio

    1971-07-01

    Seeds of the field bean variety 'Goiano Precoce' (Phaseolus vulgaris L.) subjected to various radiation doses ({sup 60}Co) ( Co) were used in a series of experiments with the objective of studying the different aspects of seed behavior thus treated. The radiation doses, comprising six treatments, varied from 0,0 to 6,4 krad of gamma radiation. Effect on seed germination and seedling dry weight was studied by means of a factorial experiment conducted under laboratory controlled conditions. The factors used were the radiation doses and nine increasing lengths of time from date of seed irradiation. Seed vigor was determined by the rate of seedling emergence when planted in small field plots. A factorial design was used. The variables were the radiation dosages and six lengths of time elapsed since date of seed irradiation. The effect of seed irradiation on yield was evaluated by means of two randomized block design field experiments. After the seed vigor experiment was conducted infestation by the bean weevil, Acanthoscelidcs obtectus Say , was observed in irradiated seeds stored under normal conditions, indicating a relationship between radiation dosage and insect damage. An analysis was made of this effect at fourteen increasing time intervals. The analysis was made according to a factorial scheme considering as factors radiation dosage and time interval. The following conclusions could be drawn from the analysis and discussion of the results obtained: a) Seed germination was adversely affected by all radiation doses in relation to the check treatment. This effect however decreased significantly with storing time. b) Seed vigor was higher for those treated with 0,4 , 0,8 and 1,6 krad when compared with those that were not irradiated. c) Pod and seed weight were lowered by the 1,6 and 6,4 krad radiation doses in relation to the check treatment. d) Infestation by the bean weevil was significantly checked by all radiation treatments in relation to the check treatment

  2. Simulating 60 Co gamma irradiation systems

    International Nuclear Information System (INIS)

    Omi, Nelson M.; Rela, Paulo R.

    2000-01-01

    The use of Cadgamma, a software dedicated to simulate 60 Co gamma irradiation systems, can lead to an optimized process and simulating, in a few hours, many configurations setups for the irradiation elements. The software can also simulate changes in the path of the product and the influence of any steady body like the support of the product support and source shoulders. These simulations minimize the number of dose mapping tests in industrial applications and allow the study of unusual setups. Cadgamma was developed at IPEN to simulate it is multipurpose 60 Co irradiation system, under construction and planned to be operating by the second half of 2001. The software was used on project stage and will help to optimize the irradiation process for each product to be treated. (author)

  3. Effect of spirit irradiation with 60Co gamma-rays

    International Nuclear Information System (INIS)

    Gwardys, S.

    1975-01-01

    A few sorts of spirit were irradiated with a dose of 1 or 5 Mrad of 60 Co gamma-rays. Then the chemical composition of spirits was investigated. It was found that as a result of irradiation the content of acids, esters, acetal aldehydes and methanol increases, while the strength of higher alcohols decreases slightly. The changes of compounds content in particular spirits are dependent on radiation doses and chemical composition before irradiation. It was also discovered that spirit irradiation causes decrease or even disappearance of characteristic - for given spirits - maxima of UV absorption. (Z.M.)

  4. Effects of 60Co gamma radiation on crotamine

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    Boni-Mitake M.

    2001-01-01

    Full Text Available Ionizing radiation can change the molecular structure and affect the biological properties of biomolecules. This has been employed to attenuate animal toxins. Crotamine is a strongly basic polypeptide (pI 10.3 from Crotalus durissus terrificus venom composed of 42 amino acid residues. It induces skeletal muscle spasms leading to a spastic paralysis of hind limbs in mice. The objective of the present study was to carry out a biochemical study and a toxic activity assay on native and irradiated crotamine. Crotamine was purified from C.d. terrificus venom by Sephadex G-100 gel filtration followed by ion-exchange chromatography, and irradiated at 2 mg/ml in 0.15 M NaCl with 2.0 kGy gamma radiation emitted by a 60Co source. The native and irradiated toxins were evaluated in terms of structure and toxic activity (LD50. Irradiation did not change the protein concentration, the electrophoretic profile or the primary structure of the protein although differences were shown by spectroscopic techniques. Gamma radiation reduced crotamine toxicity by 48.3%, but did not eliminate it.

  5. Effects of {sup 60} Co gamma radiation on crotamine

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    Boni-Mitake, M. [Instituto de Pesquisas Energeticas e Nucleares (IPEN), Sao Paulo, SP (Brazil). Dept. de Radioprotecao Ocupacional; Costa, H.; Spencer, P.J. [Instituto de Pesquisas Energeticas e Nucleares (IPEN), Sao Paulo, SP (Brazil). Dept. de Bioengenharia; Rogero, J.R [Instituto de Pesquisas Energeticas e Nucleares (IPEN), Sao Paulo, SP (Brazil). Diretoria de Materiais; Vassilieff, V.S. [UNESP, Botucatu, SP (Brazil). Instituto de Biociencias. Ceatox - Centro de Assistencia Toxicologica]. E-mail: mbmitake@net.ipen.br

    2001-12-01

    Ionizing radiation can change the molecular structure and affect the biological properties of biomolecules. This has been employed to attenuate animal toxins. Crotamine is a strongly basic polypeptide (p I 10.3) from Crotalus durissus terrificus venom composed of 42 amino acid residues. It induces skeletal muscle spasms leading to a spastic paralysis of hind limbs in mice. The objective of the present study was to carry out a biochemical study and a toxic activity assay on native and irradiated crotamine. Crotamine was purified from C.d. terrificus venom by Sephadex G-100 gel filtration followed by ion-exchange chromatography, and irradiated at 2 mg/ml in 0.15 M NaCl with 2.0 kGy gamma radiation emitted by a 60 Co source. The native and irradiated toxins were evaluated in terms of structure and toxic activity (Ld50). Irradiation did not change the protein concentration, the electrophoretic profile or the primary structure of the protein although differences were shown by spectroscopic techniques. Gamma radiation reduced crotamine toxicity by 48.3%, but did not eliminate it. (author)

  6. Qualities of Patin Fishball Irradiated by Gamma Rays (60Co)

    International Nuclear Information System (INIS)

    Yarosita FS; Bustami I; Winarti Z; Rindy PT

    2004-01-01

    An experiment on patin fishball quality using gamma irradiation ( 60 Co) has been conducted. Samples were irradiated at 0, 1, 3 and 5 kGy and stored in refrigerator at temperature 10 o C for sixty days. Samples were analysed every fifteen days, except content of fat and protein that analysed only at the beginning and the end of storage. The purpose of this experiment is to know the quality changes of patin fishball irradiated during storage, by measuring of chemical (content of fat, protein, water, TVB value, pH value) and microbiology (TPC aerobic and anaerobic bacteria) changes. The results showed that irradiation did not affect macro nutrient contents (content of fat, protein and water) of patin fishball during storage but irradiation can affect TVB and pH values. Irradiation at 1 kGy can reduce one logarithmic cycle of total aerobic and anaerobic bacteria. The storage life of irradiated patin fishball treated at 1, 3 and 5 kGy could be extended up to 15, 30 and 60 days, respectively. Control samples the storage life could be extended less than 15 days. (author)

  7. Mantle treatment of Hodkin's disease with 60Co gamma rays

    International Nuclear Information System (INIS)

    Svahn-Tapper, G.

    1979-01-01

    60 Co mantle beams were used in the radiation treatment of patients| with Hodgkin's disease. A technique using beam-flattening filters is described. The dosimetry was based on dose plans drawn up manually for each patient. The good dosimetric accuracy of the method was verified by in vivo measurements of absorbed dose at the oesophagi of 60 patients. Split-course treatment with 40 Gy was given in 27 fractions over 71 days. Results and side-effects in 90 patients are analyzed. The local recurrence rate was 3 %. (author)

  8. An industrial 60Co gamma irradiation facility in China

    International Nuclear Information System (INIS)

    Jin Yongling; Zhou Ruiying; Wang Binglin

    1990-01-01

    The radiation processing has developed very fast in China since 1980. There are more than 100 of 60 Co radiation facilities having been set up. Majority of them was with relatively small capacity and was used mainly for the laboratory study. An industrial radiation facility has been established and put into operation in Beijing Radiation Application Center (BRAC) recently. The project was based on the research works of irradiation technology during the period of 1980-1985. The main pre-research work was radiation sterilization of medical products and food irradiation. This work has obtained an award from State Commission of Science and Technology. The Commission has arranged the construction of an industrial sterilization plant for medical products as a major import project. The project has also been supported by IAEA as project CPR/8/002. The project includes a plant of production of 100,000,000 needles per year, a plant of production of 50,000,000 syringes per year and an automatic irradiation plant. All three parts have been set up now. The maximum capacity of 60 Co sources is 3.7 x 10 16 Bq, the first-time-loaded source is 12.21 x 10 15 Bq. (author)

  9. Effect of /sup 60/Co. gamma. -rays on polyphenyl methacrylate obtained by. gamma. -ray irradiation

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    Raghunath, S.; Rao, M.H.; Rao, K.N. (Bhabha Atomic Research Centre, Bombay (India). Chemistry Div.)

    1983-01-01

    Polyphenyl methacrylate of different molecular weights Msub(n) 13000, 34500 and crosslinked polymer prepared by /sup 60/Co ..gamma..-irradiation has been irradiated with /sup 60/Co ..gamma..-rays at 300 K. G(scission) of the 13000 molecular weight polymer was 15. The crosslinked polymer undergoes both degradation and crosslinking. G(x) value determined for that polymer is approx. 9. The gaseous product consists largely of CO with some CO/sub 2/ and H/sub 2/. Hydrocarbon gases were not detected. The yield of H/sub 2/ is only to the extent of 2 to 5% of the total gas and it is assumed that majority of scission takes place at the ester linkage. On the basis of the estimation of phenol and benzene, a mechanism for degradation and crosslinking is proposed.

  10. Disturbed tooth formation by 60Co-gamma-ray radiation

    International Nuclear Information System (INIS)

    Doh, Akio

    1982-01-01

    The molar of guinea pigs was irradiated with 60 Co-#betta# ray for daily observations of the manifestation of disturbed tooth formation by microradiography and the time registration by tetracycline-labelling. Irradiation first injured young blast cells of the dentin in the growth phase, dental pulp cells, and cells of the enamel. The portion composed of injured cells formed a depressed ''constriction'' from the dental pulp side toward the border between the enamel and dentin. The cells of the enamel injured by irradiation in the growth phase later formed a very thin irregular stroma. In contrast, cells in the differentiation or subsequent phase at the time of irradiation and cells probably having started to grow after irradiation proceeded with formation of a normal stroma and calcification. No uniform relation was obtained between the histological staining of the organic stroma of normal or abnormal dentin and calcification. Labelling with tetracycline revealed that the irradiation conditions in the present study provoked hardly any changes in the speed of tooth eruption. (Chiba, N.)

  11. Effect of 60 Co gamma radiation on crystalline proteins

    International Nuclear Information System (INIS)

    Bernardes, D.M.L.

    1991-01-01

    In order to study the effects of 6 0 Co gamma radiation on crystalline proteins an in vitro system was set up. For that, aqueous solutions from bovine crystalline were used irradiated with 0, 5.000, 10.000, 15.000, 20.000 and 25.000 Gy. The treatment led to protein alterations determined by different methods. By turbidimetry the formation of aggregates that increased with the radiation dose was revealed. The same observation was done from viscosity data and from the UV spectrum of the samples. From amino acid analysis and fluorimetry determinations, tryptophan appeared as the most sensitive amino acid. An increase in the free-S H-groups was also observed. After the standardization of the method, the radio modifier capability of glutathione, amino ethyl thiourea, mercapto ethyl alanine and dimethyl sulfoxide was tested. The results showed that in the presence of those substances the radiation effect was diminished. (author)

  12. Study on the energy dependence of gamma radiation detectors for 137Cs and 60Co

    International Nuclear Information System (INIS)

    Nonato, Fernanda B.C.; Diniz, Raphael E.; Carvalho, Valdir S.; Vivolo, Vitor; Caldas, Linda V.E.

    2009-01-01

    38 Geiger-Mueller radiation detectors and 9 ionization chambers were calibrated, viewing to study the energy dependence of the monitor response for gamma radiation fields ( 137 Cs and 60 Co). The results were considered satisfactory only for ionization chambers and for some Geiger-Mueller detectors

  13. Studies of natural and 60Co gamma radio-induced conduction in metaphosphate glasses and silica

    International Nuclear Information System (INIS)

    Mengual Gil, M.A.

    1977-01-01

    A study of natural and 60 Co gamma radio-induced conduction in metaphosphate glasses and silica is presented. The experimental study of natural conduction current in metaphosphate glasses in function of temperature enables to observe two different values of the activation energies in the respective temperature ranges T>223K and T [fr

  14. Different gamma ray (60 Co) dose effects on Sorghum genotype germination

    International Nuclear Information System (INIS)

    Tabosa, Jose Nildo; Gomes, Roberto Vicente; Reis, Odemar Vicente dos; Colaco, Waldeciro

    2004-01-01

    In agriculture, applying irradiation is a very valuable way of obtaining vegetable products for human and animal consumption. Cobalt-60, one of the main sources of gamma-rays, is considered an important tool in plant breeding programs, which have the objective of promoting genetic variability of cultivars with resistance to adverse environments. In this research, the effects of different 60 Co doses on germination vigor and seed germination velocity of forage sorghum genotypes were evaluated. The study was carried out at the IPA (Empresa Pernambucana de Pesquisa Agropecuaria) in Recife, Pernambuco, Brazil. The work was installed in germination boxes, following laboratory recommendations. Thus, a experiment involving three sorghum genotypes (IPA 467-4-2, IPA 02-03-01, and Sudan 4202), five 60 Co doses (Zero, 150, 300, 350, and 400 Gy), was set up. The sees were irradiated before the beginning of the experiment being exposed to gamma rays from a 60 Co-source (cobalt irradiator) at DEN (Nuclear Engineering Department) of the UFPE (Pernambuco Federal University), Brazil. The work also had the objective of evaluating the sorghum genotypes x 60 Co dose interaction. The main results obtained showed that the sorghum genotype IPA 02-03-01 presented the greatest values of germination and vigor percentages, and seed germination velocity, when compared to the others evaluated, on 350 and 400 Gy of 60 Co doses. (author)

  15. Responses of commercial broiler chicks to 60Co gamma-rays

    International Nuclear Information System (INIS)

    Shebaita, M.K.; Kamar, G.A.R.; El-Barkouky, E.E.M.

    1981-01-01

    A total of 360 chicks of Nichols line at one-day old were used in this study. Within each sex, the chicks were divided into three exposure treatments (non-irradiated, 400 rads and 800 rads). The source of irradiation was 60 Co gamma-rays. The results can be summarized as follows: Of the 360 chicks used in this study, only 10 chicks died during the experimental period. Radiation induced a reduction in body weight in both sexes during the growth period. Gamma-rays reduce the feed intake in both sexes. Moreover, the feed conversion ratio for the irradiated chicks was higher than that the non-irradiated birds. While red blood cells and hemoglobin content were higher in the irradiated chicks, white blood cells count and especially lymphocytes did not affect by 60 Co gamma-rays. Gamma-rays had no effect on carcass, bone, meat and giblets as fractions of body weight in both sexes. Moreover, meat water content decreased and meat fat content increased in irradiated chicks. The total length and the total weight of the GI tract decreased due to 60 Co gamma-rays. The response of the different regions of the GI tract to this rays is different. Moreover, the effect of this radiation on the GI tract is dependent upon the body weight irrespective of the sex. (orig./MG) [de

  16. Responses of commercial broiler chicks to /sup 60/Co gamma-rays

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    Shebaita, M.K.; Kamar, G.A.R.; El-Barkouky, E.E.M.

    1981-12-01

    A total of 360 chicks of Nichols line at one-day old were used in this study. Within each sex, the chicks were divided into three exposure treatments (non-irradiated, 400 rads and 800 rads). The source of irradiation was /sup 60/Co gamma rays. The results can be summarized as follows: Of the 360 chicks used in this study, only 10 chicks died during the experimental period. Radiation induced a reduction in body weight in both sexes during the growth period. Gamma rays reduce the feed intake in both sexes. Moreover, the feed conversion ratio for the irradiated chicks was higher than that the non-irradiated birds. While red blood cells and hemoglobin content were higher in the irradiated chicks, white blood cells count and especially lymphocytes were not affected by /sup 60/Co gamma rays. Gamma rays had no effect on carcass, bone, meat and giblets as fractions of body weight in both sexes. Moreover, meat water content decreased and meat fat content increased in irradiated chicks. The total length and the total weight of the GI tract decreased due to /sup 60/Co gamma rays. The response of the different regions of the GI tract to these rays is different. Moreover, the effect of this radiation on the GI tract is dependent upon the body weight irrespective of the sex.

  17. Analysis of the microbial growth in 60Co gamma-irradiated foods by calorimetry

    International Nuclear Information System (INIS)

    Furuta, M.; Hamasaki, K.; Wirkner, S.; Constantinoiu, E.; Takahashi, K.; Hayashi, T.

    2002-01-01

    Using a heat conduction calorimeter equipped with 24 sample units the heat evolutions from growing 60Co gamma-irradiated bioburden of black pepper seeds and frozen beef were detected in the form of growth thermograms. 60Co gamma-irradiation affected the growth pattern in which a dose-dependent reduction of the growth rate constant was observed together with the retardation in growth, indicating a combination of bactericidal and bacteriostatic effects. We successfully determined the minimal inactivation doses for the two food samples using the relationship between the irradiation dose and the retardation in growth talpha, or the growth rate constant mu obtained from the growth thermograms. These results strongly suggested the possibility of calorimetry as a measure of predictive microbiology in food irradiation

  18. Dosimetric response of united, commercially available CTA foils for 60Co gamma rays

    International Nuclear Information System (INIS)

    Peimel-Stuglik, Z.

    2001-01-01

    The usefulness of two kinds of untinted CTA foils: Fuji CTR-125 dosimetric foil and technical CTA-T foil, produced by 'Zaklady Chemiczne, 'Gorzow Wielkopolski' as support for light-sensitive layers of amateur photo-films, for 60 Co gamma ray dosimetry was investigated. In spite of rather bad physical parameters of the technical foil (spread of foil thickness, high and different initial absorbance) the dosimetric response of both foils for 60 Co gamma rays was similar. The CTA-T foil can be used for routine dosimetry providing that dosimetric signals have to be calculated exactly as recommended by the ASTM (American Society for Testing and Materials) standard, i.e. as the difference of absorbance of irradiated and (the same) non-irradiated foil. Any other approach may lead to high errors of dose evaluation. The last is true also for other CTA foils, especially after long self-life. (author)

  19. [Chinese medicinal monomer and compound for 60Co-gamma-induced spermatogenic disturbance in mice].

    Science.gov (United States)

    Zhang, Wei-xing; Wang, Hua-li; Wang, Rui; Li, Rui; He, Wei; Zhang, Tian-biao

    2010-05-01

    To explore the effects of the monomer and compound of the Chinese herbal drugs resveratrol, lycium barbarum polysaccharide (LBP) and icariin on 60Co-gamma-induced spermatogenic disturbance in mice based on the theory of modern Chinese medicine. A total of 105 male Kunming mice were randomly divided into seven groups, with 15 in each. Group A were normally raised and Groups B, C, D, E, F and G irradiated by 60Co-gamma 6 Gy followed by 60Co-gamma 4 Gy at the interval of 7 days. A week later, Groups C, D, E, F and G received intragastrically the suspension of resveratrol, resveratrol + LBP, resveratrol + icariin, resveratrol + LBP + icariin and resveratrol + LBP + icariin + L-carnitine, respectively, at the dose of 80 mg/(kg x d) for 60 days. The general condition, physical signs and body weight changes of the mice were recorded, and 24 hours after the intragastric medication, their testes were harvested to obtain the testicular weight and indexes, the levels of FSH, LH, T and E2 determined by ELISA, the T/E2 ratio calculated, and the histology of the testis tissues observed under the microscope. The testicular indexes of the mice were decreased by radiation-induced damage, but restored to some extent after intragastric medication, especially in Groups E, F and G. The levels of FSH, LH and T were obviously improved by LBP. The T level and testis weight were increased by Icariin. The level of T/E2 was elevated in Groups E, F and G. The best results were achieved in Group F, which exhibited almost complete recovery from reproductive endocrine disorder and spermatogenic damage. The Chinese medicinal monomer is effective for 60Co-gamma-induced spermatogenic disturbance in mice, and the compound suspension of resveratrol + LBP + icariin produces the best result.

  20. A comparison of ionizing radiation damage in CMOS devices from 60Co gamma rays, electrons and protons

    International Nuclear Information System (INIS)

    He Baoping; Yao Zhibin; Zhang Fengqi

    2009-01-01

    Radiation hardened CC4007RH and non-radiation hardened CC4011 devices were irradiated using 60 Co gamma rays, 1 MeV electrons and 1-9 MeV protons to compare the ionizing radiation damage of the gamma rays with the charged particles. For all devices examined, with experimental uncertainty, the radiation induced threshold voltage shifts (ΔV th ) generated by 60 Co gamma rays are equal to that of 1 MeV electron and 1-7 MeV proton radiation under 0 gate bias condition. Under 5 V gate bias condition, the distinction of threshold voltage shifts (ΔV th ) generated by 60 Co gamma rays and 1 MeV electrons irradiation are not large, and the radiation damage for protons below 9 MeV is always less than that of 60 Co gamma rays. The lower energy the proton has, the less serious the radiation damage becomes. (authors)

  1. 60Co gamma radiation effect on AlGaN//AlN/GaN HEMT devices

    International Nuclear Information System (INIS)

    Wang Yanping; Luo Yinhong; Wang Wei; Zhang Keying; Guo Hongxia; Guo Xiaoqiang; Wang Yuanming

    2013-01-01

    The testing techniques and experimental methods of the 60 Co gamma irradiation effect on AlGaN/AlN/GaN high electron mobility transistors (HEMTs) are established. The degradation of the electrical properties of the device under the actual radiation environment are analyzed theoretically, and studies of the total dose effects of gamma radiation on AlGaN/AlN/GaN HEMTs at three different radiation bias conditions are carried out. The degradation patterns of the main parameters of the AlGaN/AlN/GaN HEMTs at different doses are then investigated, and the device parameters that were sensitive to the gamma radiation induced damage and the total dose level induced device damage are obtained. (authors)

  2. Comparison of two methods of therapy level calibration at 60Co gamma beams

    International Nuclear Information System (INIS)

    Bjerke, H.; Jaervinen, H.; Grimbergen, T.W.M.; Grindborg, J.E.; Chauvenet, B.; Czap, L.; Ennow, K.; Moretti, C.; Rocha, P.

    1998-01-01

    The accuracy and traceability of the calibration of radiotherapy dosimeters is of great concern to those involved in the delivery of radiotherapy. It has been proposed that calibration should be carried out directly in terms of absorbed dose to water, instead of using the conventional and widely applied quantity of air kerma. In this study, the faithfulness in disseminating standards of both air kerma and absorbed dose to water were evaluated, through comparison of both types of calibration for three types of commonly used radiotherapy dosimeters at 60 Co gamma beams at a few secondary and primary standard dosimetry laboratories (SSDLs and PSDLs). A supplementary aim was to demonstrate the impact which the change in the method of calibration would have on clinical dose measurements at the reference point. Within the estimated uncertainties, both the air kerma and absorbed dose to water calibration factors obtained at different laboratories were regarded as consistent. As might be expected, between the SSDLs traceable to the same PSDL the observed differences were smaller (less than 0.5%) than between PSDLs or SSDLs traceable to different PSDLs (up to 1.5%). This can mainly be attributed to the reported differences between the primary standards. The calibration factors obtained by the two methods differed by up to about 1.5% depending on the primary standards involved and on the parameters of calculation used for 60 Co gamma radiation. It is concluded that this discrepancy should be settled before the new method of calibration at 60 Co gamma beams in terms of absorbed dose to water is taken into routine use. (author)

  3. Comparison of two methods of therapy level calibration at 60Co gamma beams

    International Nuclear Information System (INIS)

    Bjerke, H; Jaervinen, H; Grimbergen, T W M; Grindborg, J-E; Chauvenet, B; Czap, L; Ennow, K; Moretti, C; Rocha, P

    1998-01-01

    The accuracy and traceability of the calibration of radiotherapy dosimeters is of great concern to those involved in the delivery of radiotherapy. It has been proposed that calibration should be carried out directly in terms of absorbed dose to water, instead of using the conventional and widely applied quantity of air kerma. In this study, the faithfulness in disseminating standards of both air kerma and absorbed dose to water were evaluated, through comparison of both types of calibration for three types of commonly used radiotherapy dosimeters at 60 Co gamma beams at a few secondary and primary standard dosimetry laboratories (SSDLs and PSDLs). A supplementary aim was to demonstrate the impact which the change in the method of calibration would have on clinical dose measurements at the reference point. Within the estimated uncertainties, both the air kerma and absorbed dose to water calibration factors obtained at different laboratories were regarded as consistent. As might be expected, between the SSDLs traceable to the same PSDL the observed differences were smaller (less than 0.5%) than between PSDLs or SSDLs traceable to different PSDLs (up to 1.5%). This can mainly be attributed to the reported differences between the primary standards. The calibration factors obtained by the two methods differed by up to about 1.5% depending on the primary standards involved and on the parameters of calculation used for 60 Co gamma radiation. It is concluded that this discrepancy should be settled before the new method of calibration at 60 Co gamma beams in terms of absorbed dose to water is taken into routine use

  4. Effect of sup 60 Co-gamma radiation on the properties of furs

    Energy Technology Data Exchange (ETDEWEB)

    Raina, R.K.; Wali, B.K. (Regional Research Lab., Srinagar (India)); Wani, A.M. (Nuclear Research Lab., Srinagar (India))

    1990-01-01

    Furs pretanned with various combinations of vegetable tanning agents and retanned with alum have been irradiated with {sup 60}Co {gamma}-radiation in the dose range 5.0-114.0 kGy. The physico-chemical modifications induced by the radiation have been assessed by measuring changes in tensile strength, absorption of water, elongation and shrinkage temperature. For investigations, samples have been taken from the same topographic region of the rabbit furs, belonging to the same age and sex. The results are discussed. (author).

  5. Hypodontia in the beagle after perinatal whole-body 60Co gamma irradiation

    International Nuclear Information System (INIS)

    Lee, A.C.; Angleton, G.M.; Benjamin, S.A.

    1989-01-01

    As part of a long-term study to evaluate health effects of pre- and postnatal irradiation, dental development was examined. Beagles were irradiated in utero at 8, 28, or 55 days postcoitus or postnatally at 2, 70, or 365 days postpartum. Whole-body 60 Co gamma radiation doses ranged from 0 to 3.8 Gy. There was an age-dependent dose-related increase in premolar hypodontia for animals irradiated at 55 days postcoitus or 2 days postpartum with doses of 0.83 Gy or higher and for those irradiated at 28 days postcoitus with 1.2 Gy or higher

  6. Effect of 60Co-gamma radiation on the binding properties in furs

    International Nuclear Information System (INIS)

    Raina, R.K.

    1992-01-01

    New Zealand white rabbit pelts were pickled by the usual procedure and were tanned with basic aluminium sulphate, basic chromium sulphate and their combinations. Tanned furs were irradiated with 60 Co-gamma radiations in the dose range of 5.0-114.0 kGy. The effect of radiation on the binding properties of various added substances like mineral tannins, fats, moisture and shrinkage temperature has been assessed by their comparison with the control samples. The results of these investigations show that radiation on furs causes detannage, increases the moisture and bound fat content and decreases the shrinkage temperature of the furs. (author)

  7. Effect of [sup 60]Co-gamma radiation on the binding properties in furs

    Energy Technology Data Exchange (ETDEWEB)

    Raina, R K [Regional Research Lab., Srinagar (India)

    1992-09-01

    New Zealand white rabbit pelts were pickled by the usual procedure and were tanned with basic aluminium sulphate, basic chromium sulphate and their combinations. Tanned furs were irradiated with [sup 60]Co-gamma radiations in the dose range of 5.0-114.0 kGy. The effect of radiation on the binding properties of various added substances like mineral tannins, fats, moisture and shrinkage temperature has been assessed by their comparison with the control samples. The results of these investigations show that radiation on furs causes detannage, increases the moisture and bound fat content and decreases the shrinkage temperature of the furs. (author).

  8. Micronuclei induced by fast neutrons versus 60Co gamma-rays in human peripheral blood lymphocytes.

    Science.gov (United States)

    Vral, A; Verhaegen, F; Thierens, H; De Ridder, L

    1994-03-01

    Here we compared the effectiveness of neutrons ( = 5.5 MeV) versus 60Co gamma-rays in producing micronuclei (MN) in human lymphocytes. To obtain dose-response data, blood samples of six donors were irradiated with doses ranging from 0.1 to 5 Gy for gamma-rays and 0.1-3 Gy for neutrons. A linear dependence of MN yield with dose was found for fast neutrons while for gamma-rays a nonlinear dependence existed. For both radiation qualities no significant interindividual differences were found. Derived relative biological effectiveness values decreased with increasing dose. The MN frequency distributions were overdispersed with respect to the Poisson distribution, with neutrons showing higher dispersion values than with gamma-rays. To compare the repair kinetics of both radiation qualities split-dose experiments were performed. A dose of 4 Gy gamma-rays (3 Gy neutrons) was delivered either as a single exposure or in two equal fractions separated by time intervals ranging from 30 min to 10 h (30 min to 7 h for neutrons). The data showed for gamma-rays a significant decline (30% +/- 10%) in MN yield with interfraction time due to repair of DNA damage. This repair is a continuous process starting almost immediately after the first of the two doses and lasting 3-5 h. For fast neutrons no decline was observed indicating irreparable damage.

  9. Study on shelf life extension of papayas irradiated by /sup 60/Co gamma-rays

    Energy Technology Data Exchange (ETDEWEB)

    Chang, M.S.; Chen, M.D.; Lin, C.T.; Fu, Y.K.

    1984-11-01

    Papayas are one of the main fresh fruits in Taiwan area. Papaya fruits were treated with hot water to pasteurize peels followed by /sup 60/Co gamma-ray irradiation to extend the ripening time. The purpose of synergetic methods is to extend the shelf life of papaya fruits. This experiment was carried out by seven treatments, which were: (1) control group, (2) hot water treatment only, (3) hot water treatment with a 25 krad ..gamma..-irradiation, (4) hot water treatment with a 50 krad ..gamma..-irradiation, (5) hot water treatment with a 75 krad ..gamma..-irradiation, (6) 75 krad ..gamma..-irradiation only, and (7) hot water treatment with a 100 krad ..gamma..-irradiation. The items of observation were: surface yellowing, surface decaying, quality of texture, and length of period lasted after irradiation for 50% marketable papayas. The results of this study showed that a shelf-life extension of six days could be obtained for papayas subjected to hot water (50 to 55/sup 0/C) treatment and a 100 krad irradiation. 3 refs., 2 figs., 2 tabs.

  10. Radiation quality of tritium: a comparison with 60Co gamma rays.

    Science.gov (United States)

    Chen, Jing

    2013-09-01

    In a previous study, microdosimetric simulations were performed for tritium uniformly distributed in a medium, and for tritium bound to biologically critical sites of dimensions from 10 nm to 2 µm. Results of local energy density, i.e. energy deposition in microscopic regions, are different for these two cases. Based on the spatial distribution of energy deposition, dose mean lineal energies are calculated for tritium in the forms of tritiated water (HTO) and organically bound tritium (OBT). The dose mean lineal energies of OBT are about a factor of 1.7 higher than those of HTO in a wide range of target dimensions of biological interest. The results are consistent with radiobiological findings that OBT is about twice as effective as HTO. In this study, the same calculations were performed for (60)Co gamma rays in a wide range of target dimensions of biological interest (10 nm to 2 µm). Compared with (60)Co gamma rays, the estimated relative biological effectiveness could vary from 1.3 to 3.5 for HTO, and 2.3 to 5.6 for OBT. The results are consistent with radiobiological findings for various biological endpoints in different biological systems that OBT is about twice as effective as HTO.

  11. Degradation Of α-Chitosan By Combined Treatment With Hydroperoxide And Gamma 60Co Radiation

    International Nuclear Information System (INIS)

    Nguyen Quoc Hien; Dang Van Phu; Bui Phuoc Phuc; Ha Thuc Huy

    2008-01-01

    Chitosan samples prepared from shrimp shell (α-chitosan) with degree of deacetylation (DD) of 70, 84 and 94% were treated with H 2 O 2 1.5% at room temperature for degradation. The oxidative chitosan was irradiated by gamma 60 Co radiation for further reduction of molecular weight. Viscosity-average molecular weight of chitosan was measured by capillary viscometer. Results showed that H 2 O 2 was an effective reagent for chitosan degradation. Radiation degradation yield (G s ) increased for chitosan with higher DD. Interestingly Gs-values for oxidative degraded chitosan were found out of 0.96, 5.73 and 7.80 scissions/100 eV that were remarkably higher compared to 0.20, 1.05 and 1.69 scissions/100 eV for initial chitosan with DD 70, 84 and 94%, respectively. Based on results obtained it can be concluded that combined treatment with hydroperoxide and gamma 60 Co radiation was remarkably effective for degradation of chitosan. (author)

  12. Electrical characteristics of {sup 60}Co {gamma}-ray irradiated MIS Schottky diodes

    Energy Technology Data Exchange (ETDEWEB)

    Tataroglu, A. [Department of Physics, Faculty of Arts and Sciences, Gazi University, 06500 Ankara (Turkey)]. E-mail: ademt@gazi.edu.tr; Altindal, S. [Department of Physics, Faculty of Arts and Sciences, Gazi University, 06500 Ankara (Turkey)

    2006-11-15

    In order to interpret the effect of {sup 60}Co {gamma}-ray irradiation dose on the electrical characteristics of MIS Schottky diodes, they were stressed with a zero bias at 1 MHz in dark and room temperature during {gamma}-ray irradiation and the total dose range was 0-450 kGy. The effect of {gamma}-ray exposure on the electrical characteristics of MIS Schottky diodes has been investigated using C-V and G/{omega}-V measurements at room temperature. Experimental results show that {gamma}-ray irradiation induces a decrease in the barrier height {phi} {sub B} and series resistance R {sub s}, decreasing with increasing dose rate. Also, the acceptor concentration N {sub A} increases with increasing radiation dose. The C-V characteristics prove that there is a reaction for extra recombination centers in case of MIS Schottky diodes exposed to {gamma}-ray radiation. Furthermore, the density of interface states N {sub ss} by Hill-Coleman method increases with increasing radiation dose. Experimental results indicate that the interface-trap formation at high irradiation dose is reduced due to positive charge build-up in the Si/SiO{sub 2} interface (due to the trapping of holes) that reduces the flow rate of subsequent holes and protons from the bulk of the insulator to the Si/SiO{sub 2} interface.

  13. Perna perna (LINNAEUS, 1758) mussels irradiated by 60CO gamma rays cytotoxicity evaluation

    International Nuclear Information System (INIS)

    Martini, Gisela A.; Pusceddu, Fabio H.; Rogero, Sizue O.; Rogero, Jose Roberto

    2013-01-01

    The aim of the present work was the study of ionizing radiation effects on aquatic biota regarding the location of nuclear facilities nearby coastal areas assuming the risk of leaks and nuclear accidents. Bivalve mollusks have been widely used in the monitoring of aquatic environment studies mainly for their sessile habit and pollutants bioconcentration ability. So marine mussel Perna perna (Bivalvia: Mytilidae) was used as organism test in this study. The study of radioactive toxicity was performed by cytotoxicity test exposing the organisms to 11Gy gamma radiation dose. After radiation the neutral red retention assay evaluated the lysosomal membrane integrity in the mussel hemocytes. 50% lethal dose assay (LD50) of gamma radiation on Perna perna mussels was carried out by exposure the organisms to 60 Co gamma rays at doses ranging from 0 to 3000 Gy. The result of gamma radiation LD50 for these mussels was 1068 Gy and the neutral red retention time of irradiated organisms was about 47% lower than the control, non irradiated organisms. With the obtained results is expected to contribute in the study to identify the range of ionizing radiation doses which can cause toxic effects in marine invertebrates. (author)

  14. Some gamma radiation (60Co) effects on Chrysomya megacephala (Fabricius, 1794) (Diptira-Calliphoridae)

    International Nuclear Information System (INIS)

    Garcia, C.R.

    1987-03-01

    The effect of different gama radiation ( 60 Co) doses on Chrysomya megcephala (Fabricius) have been studied under laboratory conditions, in the Entomology Section of Centro de Energia Nuclear na Agricultura (CENA-USP), Piracicaba, SP, Brasil. The insects were collected close to sucking animals brooding areas, identified and reared on artificial diet, being kept in a temperature (26 +- 2 0 C) and humidity (66 +- 9%) controlled room, with 12L:12D photopheriod. A Gammabeam type 650 Cobalt-60 gamma irradiation source was used, with 28, 196x10 10 Bq activity at the beginning of the experiment. The pupae were submitted to increasing gamma radiation doses. To determine lethal doses, treatments were carried out at four different ages and to determine the sterilizing doses two-three day pupae were used. It was noted pupae radiosensitivity decreased with age, LD 50 varying from 14 - 513 Gy. The sterilizing dose for males was 40 Gy and for females 30 Gy. During the individuals life cycle, it was noted that there was a decrease in life expectancy (e x o ) of adults when the gamma radiation dose was increased. Treated males were more sensitive than females (Crossed with ''normals''), there occurring a marked reduction in the number of eggs and their viability when the used gamma radiation dose was increased. (author) [pt

  15. The effect of gamma radiation from sources "6"0Co of soybean for shade tolerant

    International Nuclear Information System (INIS)

    Lilik Harsanti; Yulidar

    2016-01-01

    Gamma rays are electromagnetic waves that have a very strong penetrating power. One of the sources of gamma rays is from "6"0Co. The gamma rays have strong penetration power can be used of plant breeding to create new genetic diversity in the make of high-yielding varieties. Irradiation of gamma rays at a dose of 300 gray and 400 gray on soybean Denna 1 seed varieties has been done, then it was planted as M.1 the plants look healthy and robust and strong stems. The parameters observed plant height, sum book leaf, sum seed, sum pod and Percentage plant to live shade tolerant. The parameters observed were good: plant height doze 300 Gy (37.76 cm) and 400 gray (33.03 cm) The number of branches with doze 300 Gy (1.75) and 400 Gy (1.95), number of pod with doze 300 Gy (26.25) and 400 Gy (24.72), number of seed with doze 300 Gy (35.55 kg) and 400 Gy (33.65 kg), number of Naut with doze 300 Gy (9.28) and 400 Gy (8.53) and the percentage of plants capable of shade tolerant with doze 300 Gy (85) and 400 Gy(75). In conclusion, selection mutant lines of soybeans shade tolerant at generation M1 and next generation. (author)

  16. Perna perna (LINNAEUS, 1758) mussels irradiated by {sup 60}CO gamma rays cytotoxicity evaluation

    Energy Technology Data Exchange (ETDEWEB)

    Martini, Gisela A.; Pusceddu, Fabio H.; Rogero, Sizue O.; Rogero, Jose Roberto, E-mail: gisela.martini@usp.br [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)

    2013-07-01

    The aim of the present work was the study of ionizing radiation effects on aquatic biota regarding the location of nuclear facilities nearby coastal areas assuming the risk of leaks and nuclear accidents. Bivalve mollusks have been widely used in the monitoring of aquatic environment studies mainly for their sessile habit and pollutants bioconcentration ability. So marine mussel Perna perna (Bivalvia: Mytilidae) was used as organism test in this study. The study of radioactive toxicity was performed by cytotoxicity test exposing the organisms to 11Gy gamma radiation dose. After radiation the neutral red retention assay evaluated the lysosomal membrane integrity in the mussel hemocytes. 50% lethal dose assay (LD50) of gamma radiation on Perna perna mussels was carried out by exposure the organisms to {sup 60}Co gamma rays at doses ranging from 0 to 3000 Gy. The result of gamma radiation LD50 for these mussels was 1068 Gy and the neutral red retention time of irradiated organisms was about 47% lower than the control, non irradiated organisms. With the obtained results is expected to contribute in the study to identify the range of ionizing radiation doses which can cause toxic effects in marine invertebrates. (author)

  17. Cell survival of human tumor cells compared with normal fibroblasts following 60Co gamma irradiation

    International Nuclear Information System (INIS)

    Lloyd, E.L.; Henning, C.B.; Reynolds, S.D.; Holmblad, G.L.; Trier, J.E.

    1982-01-01

    Three tumor cell lines, two of which were shown to be HeLa cells, were irradiated with 60 Co gamma irradiation, together with two cell cultures of normal human diploid fibroblasts. Cell survival was studied in three different experiments over a dose range of 2 to 14 gray. All the tumor cell lines showed a very wide shoulder in the dose response curves in contrast to the extremely narrow shoulder of the normal fibroblasts. In addition, the D/sub o/ values for the tumor cell lines were somewhat greater. These two characteristics of the dose response curves resulted in up to 2 orders of magnitude less sensitivity for cell inactivation of HeLa cells when compared with normal cells at high doses (10 gray). Because of these large differences, the extrapolation of results from the irradiation of HeLa cells concerning the mechanisms of normal cell killing should be interpreted with great caution

  18. Effect of low-dose 60Co gamma irradiation on the photosynthetic activity of maize seedlings

    International Nuclear Information System (INIS)

    Turcsanyi, G.

    1979-01-01

    Photosynthesis investigations were carried out on maize seedlings treated with 1000 rad of 60 Co gamma irradiation prior to sowing. The aim of the work was to find out, to what degree the individual processes of photosynthesis are affected by small doses of irradiation. The increase in weight, the chlorophyll a, chlorophyll b and carotinoid content as well as the photosynthetic O 2 -evolution and 14 CO 2 -fixation of the seedlings were measured. The results indicate that, in contradiction to the data given in the literature, the occasional increase in weight caused by small-dose irradiation is not in connection with the membrane-bound part of the photosynthetic apparatus, but it is the consequence of the increased activity of the Calvin-cycle enzymes in the stroma of the chloroplasts. (author)

  19. Determination of the sterilizing gamma radiation dose of 60Co to ACANTHOSCELIDES OBTECTUS imagos (col. bruchidae)

    International Nuclear Information System (INIS)

    Arthur, V.; Wiendl, F.M.; Sgrillo, R.B.; Campanhola, C.

    1980-01-01

    This paper relates the results of experiments dealing with irradiation of adults of Acanthoscelides obtectus (Say, 1831). The insects were irradiated with doses of 0 (control), 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15 and 20 krad gamma radiation of a 60 Co source. After irradiation, the adults were kept for observation on beans (Phaseolus vulgaris) var. Jalo, in a controlled environmen t chamber at 30 +- 1 0 C and 70 +- 5% relative humidity. Continuous weighing at weekly intervals was done 22 times, showing, by weight loss in percent, that the sterilizing dose for adults was 10,65 krad. It could also be observed that losses of less than 0,5% occured at 9 krad. For a possible employment on commercial scale, the ideal radiation dose for bean desinfestation would be 10 krad, after which no weight loss occurrence. (Author) [pt

  20. Evaluation of fruit productivity and quality in Hass avocado submitted to 60Co gamma radiation

    International Nuclear Information System (INIS)

    Cruz T, E. De la; Ibanez P, J.; Mijares O, P.; Garcia A, J.M.

    1999-01-01

    Evaluation of productivity, postharvest behavior and fruit quality was performed on four years Hass avocado trees irradiated with 60 Co gamma rays in doses of 5, 10, 15, 20 and 25 Gy, established in the ''La Labor'' Experimental Center of the Centro de Investigaciones Cientificas y Tecnologias del Aguacate en el Estado de Mexico (CICTAMEX) at Temascaltepec Mexico. Productivity had a significant increase in the dose of 15 Gy being the average number of fruits nearly 400 % more than the control at fruit setting, being such difference reduced at fruit harvesting to 300 %. In regard to postharvest performance, the respiration index (mg CO 2 /kg/hr) did not show significant differences among treatments. Also others variables such as physiological weight losses, texture, maturity pattern, and sensorial tests (color, flavor, aroma, texture) were not different in regard to the control. This mean that radiation has altered productivity but not the quality and postharvest behavior of fruits. (Author)

  1. Effects of 60Co gamma-rays on some biological characteristics of Chinese hamster lung cells

    International Nuclear Information System (INIS)

    Tang Pei; Wang Shoufang; Zhang Shuxian

    1988-01-01

    The proliferation of cells and the relationship between survival and dose were investigated in Chinese hamster lung (CHL) cells grown at stationary phase and irradiated with 60 Co gamma-rays. The ultrastructural changes and chromosome aberration in the cells after irradiation were also observed. The frequency of chromosome aberrations increased linearly with dose and the yields of dicentrics plus rings were best fitted to a linear-quadratic model. The 50% growth-inhibited dose was found to be 4.0Gy. Electron microscopy observation revealed swelling and vacuolation of mitochodria and indistinct cristae at lower doses. The alterations in nucleus at higher doses appeared to be depression of nuclear membrane and disappearance of chromatin

  2. [Effect of 60Co gamma-irradiation on dilute aqueous solutions of surfactants].

    Science.gov (United States)

    Sawai, T; Shimokowa, T; Miki, Y; Oseko, K; Sawai, T

    1978-01-01

    Present work deals with the effects of gamma irradiation from 60Co gamma-ray source upon aqueous solutions of three kinds of surfactants. When dilute aqueous solutions of sodium dodecyl sulfate (SDS, anionic), cethyl trimethyl ammonium chloride (CTAC, cationic), and polyoxyethylene lauryl ether (POE, non-ionic) were irradiated with gamma-rays at a room remperature, the residual concentration, products, surface tension, and forming power were examined by colorimetric method, IR spectrophotometric method, gaschromatography, Ross-Miles method, and Traube's stalagnometer etc.. These surfactants were decomposed by the irradiation and thus the surface tension increased and the forming power, on the contrary, decreased with dose. Radiation chemical yields (G-value) of the degradation were about 1 for the solutions of SDS and CTAC, and about 0.3 for the POE solution. From the experimental results, it was found that following chemical reactions seem to occur followed by the radiolysis of water; a) bond cleavage of ester for SDS, of CN for CTAC, and of oxyethylene for POE, b) hydrogen abstraction from the surfactants, c) production of CO bond in the presence of dissolved oxygen.

  3. Ionizing radiation from {sup 60}Co and electron accelerator in reducing the population of Salmonella sp. inoculated in chicken meatballs: evaluation of acceptance by consumer;Radiacoes ionizantes provenientes de {sup 60}CO e acelerador de eletrons na reducao da populacao de Salmonella sp. inoculada em almondegas de frango: avaliacao da aceitacao do produto pelo consumidor

    Energy Technology Data Exchange (ETDEWEB)

    Saito, Juliana

    2003-07-01

    Salmonella sp. is one of the main microorganisms that causes outbreaks of food borne diseases associated to poultry, and among its derivatives, the chicken meatballs are getting the favorites of the consumer. In the attempt to improve microbiological food safety, a method that has been hardly studied is the irradiation. To study the viability of the use o ionizing radiation originated from {sup 60} Co and electrons accelerator in the reduction of Salmonella sp., frozen chickens meatballs were inoculated with 10{sup 4} CFU/g of Salmonella sp., with needle and syringe. Subsequently they were exposure to doses of 1.5, 2.0, 2.5 and 3.0 kGy and Salmonella sp. was enumerated. Chicken meatballs were exposed to doses 3.0, 4.0 and 5.0 kGy and submitted to sensory analysis. The gamma radiation from {sup 60}CO was effective in the reduction of Salmonella sp. inoculated in chicken meatballs. The ionizing radiation originated from electron beam was not effective in the conditions applied in this research. The commercial chicken meatballs prepared with chicken meat, mixed up with soy protein, seasoning and anti-oxidants additives did not loose their sensorial quality when exposure to doses of 3.0, 4.0 and 5.0 kGy and, in a general way, the consumers showed to be disposed to buy the irradiation product (author)

  4. Biochemical and pharmacological characterization of irradiated crotamine by gamma rays of 60Co

    International Nuclear Information System (INIS)

    Oliveira, Karina Corleto

    2014-01-01

    The serum production in Brazil, the only effective treatment in cases of snakebites, uses horses that although large size, have reduced l lifespan compared with horses not immunized. Ionizing radiation has been shown as an excellent tool in reducing the toxicity of venoms and toxins isolated, and promote the achievement of better immunogens for serum production, and contributing to the welfare of serum-producing animals. It is known, however, that the effects of ionizing radiation on protein are characterized by various chemical modifications, such as fragmentation, cross-linking due to aggregation and oxidation products generated by water radiolysis. However, the action of gamma radiation on toxins is not yet fully understood structurally and pharmacologically, a fact that prevents the application of this methodology in the serum production process. So we proposed in this paper the characterization of crotamine, an important protein from the venom of Crotalus durissus terrificus species, irradiated with 60 Co gamma rays. After isolating the toxin by chromatographic techniques and testing to prove the obtaining of pure crotamine, it was irradiated with gamma rays and subjected to structural analysis, Fluorescence and Circular Dichroism. Using high hydrostatic pressure tests were also conducted in order to verify that the conformational changes caused by radiation suffer modifications under high pressures. From the pharmacological point of view, muscle contraction tests were conducted with the objective of limiting the action of crotamine in smooth muscle as well as the change in the action of toxin caused structural changes to the front. Analysis of Circular Dichroism and Fluorescence showed changes in structural conformation of crotamine when subjected to gamma radiation and that such changes possibly occurring in the secondary and tertiary structure of the protein. The observed in pharmacological tests showed that the irradiated crotamine was less effective in

  5. Biochemical and pharmacological studies of native and irradiated crotamine with gamma radiation of 60Co

    International Nuclear Information System (INIS)

    Mitake, Malvina Boni

    2000-01-01

    Ionizing radiation can change the molecular structure and affect the biological properties of biomolecules. This has been employed to attenuate animal toxins. Crotamine is a strongly basic polypeptide from the South American rattlesnake venom, composed of 42 amino acid residues. It induces skeletal muscle spasms leading to a spastic paralysis of hind limbs in mice. The objective of this thesis was carry out biochemical and pharmacological studies of native and irradiated crotamine with 60 Co. Crotamine was purified from Crotalus durissus terrificus venom by Sephadex G-100 gel filtration followed by ion exchange chromatography, using a Fast Performance Liquid Chromatography (FPLC) system. It was irradiated at 2 mg/ml in 0.15 M Na Cl with 2.0 kGy gamma radiation emitted by a 60 Co source. The native and irradiated crotamine were evaluated by biochemical characterization, toxic activity (LD 50 and biodistribution. The native and irradiated crotamine were labelled with 29.6 MBq of 125 I using chloramine T method, and separated in a Sephadex G-50 column. Male Swiss mice (35± 5 g), were injected i.p. with o.1 mL (2.4 x 10 6 cpm/mouse) of 125 I native crotamine or with 0.4 mL (1.3 x 10 6 cpm/mouse) of 125 I irradiated crotamine. At 0.08; 0.25; 0.5; 1; 2; 4; 8; 12 and 24 hours the animal were killed by ether inhalation. Blood, spleen, liver, kidneys, brain, lungs, heart, and skeletal muscle were collected in order to determine radioactivity content. The results showed that gamma radiation did not change the protein concentration, the electroforetic profile or the primary structure of the protein, although differences were shown by spectroscopic techniques. The gamma radiation diminished the toxicity of crotamine, but it did not abolish bioactivity. Biodistribution studies showed that native and irradiated crotamine have hepatic metabolism and renal elimination. The native and irradiated crotamine have affinity by skeletal muscle and they did not pass the blood - brain

  6. Teratogenic effects of 60Co gamma rays irradiation on rat embryos

    International Nuclear Information System (INIS)

    Lee, Juing-Yi; Okuda, Hiroe; Tutimoto, Sigeo; Satow, Yukio

    1987-01-01

    The teratogenicity of 60 Co gamma rays was evaluated in Donryu rats. The results were compared with those of triterated water (HTO) for determining relative biological effectiveness (RBE) for incidence of malformations and LD 50 in rats. Pregnant rats were irradiated with a 60 Co source at a dose-rate of 0.5 Gy/min or 0.01 Gy/min on day 7, 8, 9, 10 or 11 of gestation with 0.8, 1.0, 1.2, 1.5, 2.0, 2.3, 2.5, 2.8 or 3.0 Gy. HTO was administered intraperitoneal injection to pregnant rats at various doses on day 7, 8, 9, 10 and 11 of gestation. The rats were sacrificed on day 18 and the offspring were examined for external and visceral malformations. Mortality, teratogenicity and effects on fetal growth were day-and dosage-dependent in both radiation groups. Congenital malformations were found most frequently in the 9-day irradiated group and followed by the 8, 11, 10 and 7-day irradiated groups. The incidence of cardiovascular anomalies was highest, especially in the day 9 of gestation group, followed by malformations in the central nervous system, craniofacial system, respiratory system, hind limbs and tail. Beta rays from HTO were found to be more effective than γ rays in inducing congenital malformations. The RBE for incidence of malformations and LD 50 was between 1.3 and 1.5. These studies suggest that simulator of tritium irradiation is urgently needed to investigate the biological effects on rats to estimate the human risks, with respect to RBE of tritium beta rays. (author)

  7. Effect of 60Co gamma irradiation on cochlea function and structure in guinea pigs

    International Nuclear Information System (INIS)

    Wang Zhonghe; Cai Yilin; Hu Haisen

    1994-01-01

    In the experiment inner ears of guinea pigs were irradiated with 60 Co gamma rays using the method of simulated clinical routine irradiation in order to find the effects of ionizing radiation on the cochlea function and structure and to explore the mechanism and protective measure that lead to hearing impairment following radiotherapy to the head and neck of cancer patients. Ten weeks after irradiation it was found that ABR threshold increased with increasing dose of radiation. The mean values of ABR thresholds in 70 Gy and 90 Gy groups were 10 dB and 28 dB respectively. The number of inner and outer hair cell and pillar cell loss was counted under microscope. The number of lost cells increased with increasing dose of radiation. The severity of damage in morphology of inner, outer hair cells and pillar cells reduced progressively. The groups of 70 Gy and 90 Gy lost significantly more than groups of control and 50 Gy(P<0.05). It is concluded that the damage of hair cells of cochlea is the direct result of gamma ray irradiation of the inner ear and is the main cause of hearing impairment following irradiation

  8. Biochemical and pharmacological characterization of irradiated crotamine by gamma rays of {sup 60}Co; Caracterizacao bioquimica e farmacologica da crotamina irradiada por raios gama de {sup 60}Co

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira, Karina Corleto

    2014-07-01

    The serum production in Brazil, the only effective treatment in cases of snakebites, uses horses that although large size, have reduced l lifespan compared with horses not immunized. Ionizing radiation has been shown as an excellent tool in reducing the toxicity of venoms and toxins isolated, and promote the achievement of better immunogens for serum production, and contributing to the welfare of serum-producing animals. It is known, however, that the effects of ionizing radiation on protein are characterized by various chemical modifications, such as fragmentation, cross-linking due to aggregation and oxidation products generated by water radiolysis. However, the action of gamma radiation on toxins is not yet fully understood structurally and pharmacologically, a fact that prevents the application of this methodology in the serum production process. So we proposed in this paper the characterization of crotamine, an important protein from the venom of Crotalus durissus terrificus species, irradiated with {sup 60}Co gamma rays. After isolating the toxin by chromatographic techniques and testing to prove the obtaining of pure crotamine, it was irradiated with gamma rays and subjected to structural analysis, Fluorescence and Circular Dichroism. Using high hydrostatic pressure tests were also conducted in order to verify that the conformational changes caused by radiation suffer modifications under high pressures. From the pharmacological point of view, muscle contraction tests were conducted with the objective of limiting the action of crotamine in smooth muscle as well as the change in the action of toxin caused structural changes to the front. Analysis of Circular Dichroism and Fluorescence showed changes in structural conformation of crotamine when subjected to gamma radiation and that such changes possibly occurring in the secondary and tertiary structure of the protein. The observed in pharmacological tests showed that the irradiated crotamine was less effective

  9. Evaluation of myotoxic activity of Bothropstoxin-1 irradiated with 60Co gamma rays

    International Nuclear Information System (INIS)

    Mirco, Jessica; Baptista, Janaina A.; Caproni, Priscila; Yoshito, Daniele; Nascimento, Nanci do

    2009-01-01

    Ionizing radiation has been successfully employed to modify the immunological properties of biomolecules and has been proven to be a powerful tool to attenuate snake venoms toxicity without affecting their immunogenic properties. Snake venoms and their isolated toxins showed structural modifications after gamma radiation process, in aqueous solution, mainly by water radiolysis sub products. Free radical scavengers, such as NaNO 3 and t-butanol, present selective effects on specific radical from water radiolysis. The NaNO 3 has affinity by aqueous electron, while the t-butanol has affinity by hydroxyl radical. At the present work, we have investigated the myotoxic activity of Bothropstoxin-1 (BTHX-1), a K49 phospholipase, present in Bothrops jararacussu crude venom, before and after irradiation process, with or without scavenger substances presence. BTHX-1 was irradiated with 2 kGy of 60 Co gamma rays, in aqueous solution and in the presence of oxygen. BALB/c mice were inoculated with either native or irradiated toxin, with or without scavenger substances. After 3 hours, blood samples were collected and the myotoxic activity was evaluated by LDH (lactate dehydrogenase) release. The muscular tissue damage was directly related to the LDH amounts released. Irradiated Bothropstoxin-1, with or without NaNO 3 substance, caused less damage than their native counterpart. But irradiated toxin, in the presence of t-butanol, was so myotoxic as the native BTHX-1. These results indicate that irradiation of toxic proteins can promote significant modifications on their structures, but still retaining many of the original biological properties of their native counterparts. Additionally, some scavengers substances can change these gamma radiation effects. (author)

  10. Evaluation of myotoxic activity of Bothropstoxin-1 irradiated with {sup 60}Co gamma rays

    Energy Technology Data Exchange (ETDEWEB)

    Mirco, Jessica; Baptista, Janaina A.; Caproni, Priscila; Yoshito, Daniele; Nascimento, Nanci do [Instituto de Pesquisas Energeticas e Nucleares (IPEN-CNEN/SP), Sao Paulo, SP (Brazil)], e-mail: jessica.mirco@yahoo.com.br, e-mail: janabap@gmail.com, e-mail: pricaproni@hotmail.com, e-mail: daniyoshito@uol.com.br, e-mail: nnascime@ipen.br

    2009-07-01

    Ionizing radiation has been successfully employed to modify the immunological properties of biomolecules and has been proven to be a powerful tool to attenuate snake venoms toxicity without affecting their immunogenic properties. Snake venoms and their isolated toxins showed structural modifications after gamma radiation process, in aqueous solution, mainly by water radiolysis sub products. Free radical scavengers, such as NaNO{sub 3} and t-butanol, present selective effects on specific radical from water radiolysis. The NaNO{sub 3} has affinity by aqueous electron, while the t-butanol has affinity by hydroxyl radical. At the present work, we have investigated the myotoxic activity of Bothropstoxin-1 (BTHX-1), a K49 phospholipase, present in Bothrops jararacussu crude venom, before and after irradiation process, with or without scavenger substances presence. BTHX-1 was irradiated with 2 kGy of {sup 60}Co gamma rays, in aqueous solution and in the presence of oxygen. BALB/c mice were inoculated with either native or irradiated toxin, with or without scavenger substances. After 3 hours, blood samples were collected and the myotoxic activity was evaluated by LDH (lactate dehydrogenase) release. The muscular tissue damage was directly related to the LDH amounts released. Irradiated Bothropstoxin-1, with or without NaNO{sub 3} substance, caused less damage than their native counterpart. But irradiated toxin, in the presence of t-butanol, was so myotoxic as the native BTHX-1. These results indicate that irradiation of toxic proteins can promote significant modifications on their structures, but still retaining many of the original biological properties of their native counterparts. Additionally, some scavengers substances can change these gamma radiation effects. (author)

  11. Effects of 2. 0 Gy of /sup 60/Co gamma rays irradiation on rat embryos

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Juing-Yi; Satow, Yukio

    1987-01-01

    Pregnant rats of Donryu strain were exposed to a whole-body /sup 60/Co ..gamma.. ray irradiation of a single dose of 2.0 Gy (Dose rate: 0.5 Gy/min) on day 7, 8, 9, 10 or 11 of gestation (sperm day = day 0). The rats were sacrificed on day 18 and the offspring were examined for external and visceral malformations. Malformed embryos occurred between days 7 and 11 with the highest incidence occurring on day 9. Dose with 2.0 Gy increased the rate of resorption or death (52.1 %), in the survivors, caused congenital malformation in a majority of embryos (86.5 %) on day 8 of gestation. There is an increase in malformation (93.3 %) and growth retardation, but no increase in mortality (42.9 %) on day 9 of gestation. Relatively few anomalies resulted from irradiation on day 7 of gestation. The peak day for cardiovascular anomalies occurred on day 9 (88.3 % of all survival embryos) with high levels also occurring on day 8 (86.5 %). Cardiovascular anomalies consisted of VSD, hypoplasia of the pulmonary trunk, coarctation of the aorta, double aortic arch, right aortic arch, riding aorta, complete transposition of the aorta, persistent atrioventricular canal, vascular ring, aberrant right subclavian artery and others. Similar anomalies, but at a lower incidence, were produced by /sup 60/Co ..gamma.. ray at dose levels of 2.0 Gy on day 10 or 11 of gestation. Cases of cleft lip and cleft palate or facial cleft were observed seventeen fetuses on day 9 of gestation (31 %). Exencephaly occurred in nine embryos treated on day 9 (16.1 %) and in one embryos treated on day 10. Tail defects appeared with treatment on day 9 with the latter predominating on day 11. The present study show that maximum resorption (52.1 %) was seen with treatment on day 8 whereas the highest rate of malformation (93.3 %) was observed with treatment on day 9. (J.P.N.).

  12. Effect of 60Co gamma-ray irradiation on dilute aqueous solutions of surfactants

    International Nuclear Information System (INIS)

    Sawai, Teruko; Shimokawa, Toshinari; Miki, Yasushi; Oseko, Koichi; Sawai, Takeshi

    1978-01-01

    Present work deals with the effects of gamma irradiation from 60 Co γ-ray source upon aqueous solutions of three kinds of surfactants. When dilute aqueous solutions of sodium dodecyl sulfate (SDS, anionic), cethyl trimethyl ammonium chloride (CTAC, cationic), and polyoxyethylene lauryl ether (POE, non-ionic) were irradiated with γ-rays at a room temperature, the residual concentration, products, surface tension, and forming power were examined by colorimetric method, IR spectrophotometric method, gaschromatography, Ross-Miles method, and Traube's stalagmometer etc. These surfactants were decomposed by the irradiation and thus the surface tension increased and the forming power, on the contrary, decreased with dose. Radiation chemical yields (G-value) of the degradation were about 1 for the solutions of SDS and CTAC, and about 0.3 for the POE solution. From the experimental results, it was found that following chemical reactions seem to occur followed by the radiolysis of water: a) bond cleavage of ester for SDS, of CN for CTAC, and of oxyethylene for POE, b) hydrogen abstraction from the surfactants, c) production of CO bond in the presence of dissolved oxygen. (auth.)

  13. The effect of perinatal 60Co gamma radiation on brain weight in beagles

    International Nuclear Information System (INIS)

    Hamilton, B.F.; Benjamin, S.A.; Angleton, G.M.; Lee, A.C.

    1989-01-01

    Beagle dogs were given single, whole-body 60 Co gamma-radiation exposures at one of three prenatal (8, 28, or 55 days postcoitus) or three postnatal (2, 70, or 365 days postpartum) ages to evaluate the relative radiosensitivity of various stages of brain development. A total of 387 dogs received mean doses ranging from 0.16 to 3.83 Gy, and 120 dogs were sham-irradiated. Groups of dogs were sacrificed at preselected times from 70 days to 11 years of age. Brain weight decreased significantly with increasing dose in dogs irradiated at 28 or 55 days postcoitus or at 2 days postpartum. Irradiations at 28 days postcoitus were dramatically more effective in causing a reduction in brain weight than those at 55 days postcoitus or 2 days postpartum. Among dogs given 1.0 Gy or more and followed for up to 4 years, there was a radiation effect evident at all three sensitive exposure ages. Among dogs given lower doses and followed for up to 11 years, there was a significant decrease in brain weight in dogs given 0.80-0.88 Gy at 28 days postcoitus. All decreases in brain weight were present after normalization for radiation-induced reductions in skeletal (body) size. No specific morphologic changes were noted in the brains which showed the radiation-related reductions in size

  14. Attenuation of 60Co gamma rays by barium acrylic resin composite shields

    International Nuclear Information System (INIS)

    Abdulla, Riaz; Fidha, Mariyam; Sripathi Rao, B.H.; Kudkuli, Jagadish; Rekha, P.D.; Sharma, S.D.

    2015-01-01

    Oral squamous cell carcinoma is the sixth most common cancer reported globally, with an annual incidence of over 300,000 cases, of which 62% arise in developing countries. Radiation therapy is a treatment modality that uses ionizing radiation as a therapeutic agent. It is widely employed in the treatment of head and neck cancer, as a primary therapy coupled with surgical procedure and chemotherapy or as a palliative treatment for advanced tumors. However, radiotherapy can cause a series of complications such as xerostomia, mucositis, osteoradionecrosis, and radiation caries. Composite circular disc containing different ratios of acrylic and barium sulfate (BaSO 4 ) were made in-house. The purpose of this study was to evaluate the percentage attenuation from these composite shields in 60 Co gamma rays. A maximum of 8% radiation attenuation was achieved using 1:4 ratio of acrylic-BaSO 4 composite shields. The study proposes BaSO 4 as one of the compounds in combination with acrylic resin or any other thermoplastic substances for making biocompatible radiation attenuating devices. (author)

  15. Gamma radiation effects of 60 Co on Bombyx mori (Lep., Bombycidae) modifying the silk fiber production

    International Nuclear Information System (INIS)

    Carneiro Junior, Francisco; Bendassolli, Jose A.

    1997-01-01

    The present work aimed to verify the biological effects of the application of different doses of gamma radiation during the fifth instar of the silkworm catepillar. Sevently eight silkworm caterpillars (Bombyx mori) were irradiated with γ 60 Co radiation at the initial period of the fifth instar. The caterpillars were divided and classified in six batches of thirteen individuals each. Treatments 1 through 5 received 20, 40, 60, 80 and 100 Gy, respectively, and the control, also consisted of thirteen caterpillars, was not irradiated. The results showed a general increase in the silk fiber content in the irradiated batches compared to the control. The weight of the silk cocoons was higher with increasing doses of irradiation, from 20 to 80 Gy, respectively, followed by a decrease in weight in the treatment irradiated with 100 Gy. the results obtained in this experiment enable the conclusion that the radiation applied to the caterpillars significantly influenced the production of silk fiber in this species. (author). 4 refs., 2 figs., 3 tabs

  16. Study of irradiated bothropstoxin-1 with 60Co gamma rays: immune system behavior

    International Nuclear Information System (INIS)

    Caproni, P.; Baptista, J.A.; Almeida, T.L. de; Nascimento, N.; Passos, L.A.C.

    2009-01-01

    Ionizing radiation has been successfully employed to modify the immunological properties of biomolecules. Very promising results were obtained when crude animal venoms, as well as isolated toxins, were treated with 60Co gamma rays, yielding toxoids with good immunogenicity. The achievement of modified antigens with lower toxicity and preserved or improved immunogenicity can be very useful. Ionizing radiation has already been proven to be a powerful tool to attenuate snake venom toxicity without affecting, and even increasing, their immunogenic properties. However, little is known about the modifications that irradiated molecules undergo and even less about the immunological response that such antigens elicit. In the present work, we investigated the immunological behavior of bothropstoxin-1, a K49 phospholipase, before and after irradiation. Structural modifications of the toxin were analyzed by SDS-PAGE. Isogenic mice were immunized with either the native or the irradiated toxin. The circulating antibodies were isotyped and titrated by ELISA. According to our data, irradiation promoted structural modifications in the toxin characterized by higher molecular weight forms of proteins (aggregates and oligomers). The results also indicated that irradiated toxins were immunogenic and antibodies elicited by them were able to recognize the native toxin in ELISA. These findings suggest that irradiation of toxic proteins can promote significant modifications in their structures; however they still retain many of the original antigenic and immunological properties of native proteins. Also, our data indicate that irradiated proteins induce higher titers of IgG2a and IgG2b, suggesting that Th1 cells are predominantly involved in the immune response. (author)

  17. Study of irradiated bothropstoxin-1 with {sup 60}Co gamma rays: immune system behavior

    Energy Technology Data Exchange (ETDEWEB)

    Caproni, P.; Baptista, J.A.; Almeida, T.L. de; Nascimento, N. [Nuclear and Energy Research Institute (IPEN/CNEN-SP), SP (Brazil). Biotechnology Center; Passos, L.A.C. [State University of Campinas (UNICAMP), SP (Brazil). Lab. of Genetics and Embryo Cryopreservation

    2009-07-01

    Ionizing radiation has been successfully employed to modify the immunological properties of biomolecules. Very promising results were obtained when crude animal venoms, as well as isolated toxins, were treated with 60Co gamma rays, yielding toxoids with good immunogenicity. The achievement of modified antigens with lower toxicity and preserved or improved immunogenicity can be very useful. Ionizing radiation has already been proven to be a powerful tool to attenuate snake venom toxicity without affecting, and even increasing, their immunogenic properties. However, little is known about the modifications that irradiated molecules undergo and even less about the immunological response that such antigens elicit. In the present work, we investigated the immunological behavior of bothropstoxin-1, a K49 phospholipase, before and after irradiation. Structural modifications of the toxin were analyzed by SDS-PAGE. Isogenic mice were immunized with either the native or the irradiated toxin. The circulating antibodies were isotyped and titrated by ELISA. According to our data, irradiation promoted structural modifications in the toxin characterized by higher molecular weight forms of proteins (aggregates and oligomers). The results also indicated that irradiated toxins were immunogenic and antibodies elicited by them were able to recognize the native toxin in ELISA. These findings suggest that irradiation of toxic proteins can promote significant modifications in their structures; however they still retain many of the original antigenic and immunological properties of native proteins. Also, our data indicate that irradiated proteins induce higher titers of IgG2a and IgG2b, suggesting that Th1 cells are predominantly involved in the immune response. (author)

  18. Histological variations in liver of freshwater fish Oreochromis mossambicus exposed to 60Co gamma irradiation

    International Nuclear Information System (INIS)

    Sadiq Bukhari, A.; Syed Mohamed, H.E.; Broos, K.V.; Stalin, A.; Singhal, R.K.; Venubabu, P.

    2012-01-01

    The irradiation effect of 60 Co at the three dose level of 3 mGy, 30 mGy and 300 mGy on the histology of liver of the freshwater fish Oreochromis mossambicus was investigated. The liver of O. mossambicus was dissected out and processed for light microscopy studies. 60 Co exposed O. mossambicus were found to result in several alterations in the histoarchitecture of liver. The alterations included mild congestion of blood vessels, structural alteration, cellular swelling, vacuolation and necrotic liver cells, indicating a definite response to 60 Co irradiation. The results suggest that the liver of O. mossambicus exposed to 60 Co were structurally altered with increasing dose levels. It is to record that alteration in the liver does not affect the physiology, behaviour or lethality of the individuals. Self regulating mechanisms would have influenced the liver to remain sustained. To confirm the same further studies in the direction by increasing dose level is required. - Highlights: ► Fish Oreochromis mossambicus irradiated to the dose of 3 mGy, 30 mGy and 300 mGy. ► Histoarchitecture of liver altered with increasing dose levels of 60 Co. ► Alteration in the liver does not affect the physiology, behaviour or lethality. ► Self regulating mechanisms might have prevented from Lethality. ► HSI index value for exposed group reported ( 60 Co.

  19. Response of avocado genotypes to improvement through {sup 60}Co gamma radiation; Respuesta de diversos genotipos de aguacate al mejoramiento por radiacion gamma de {sup 60}Co

    Energy Technology Data Exchange (ETDEWEB)

    Cruz, E De la; Rubi A, M; Garcia A, J M [Instituto Nacional de Investigaciones Nucleares, A.P. 18-1027, 11801 Mexico D.F. (Mexico)

    1997-07-01

    Ten avocado genotypes were subjected to gamma radiation from 0 to 45 Gy in 1993. Vegetative and reproductive data were analysed in a factorial design. Genotypes differed significative on height and fruit number. Radiation affected significative fruit number but not tree height. ''Hass'' showed strongest interaction between genotype and doses, for fruit number. (Author)

  20. Response of avocado genotypes to improvement through {sup 60}Co gamma radiation; Respuesta de diversos genotipos de aguacate al mejoramiento por radiacion gamma de {sup 60}Co

    Energy Technology Data Exchange (ETDEWEB)

    Cruz, E. De la; Rubi A, M.; Garcia A, J.M. [Instituto Nacional de Investigaciones Nucleares, A.P. 18-1027, 11801 Mexico D.F. (Mexico)

    1997-07-01

    Ten avocado genotypes were subjected to gamma radiation from 0 to 45 Gy in 1993. Vegetative and reproductive data were analysed in a factorial design. Genotypes differed significative on height and fruit number. Radiation affected significative fruit number but not tree height. ''Hass'' showed strongest interaction between genotype and doses, for fruit number. (Author)

  1. The role of {sup 60}Co gamma-ray irradiation on the interface states and series resistance in MIS structures

    Energy Technology Data Exchange (ETDEWEB)

    Tascioglu, Ilke [Department of Physics, Faculty of Science and Arts, Gazi University, 06500 Ankara (Turkey); Tataroglu, Adem, E-mail: ademt@gazi.edu.t [Department of Physics, Faculty of Science and Arts, Gazi University, 06500 Ankara (Turkey); Ozbay, Akif; Altindal, Semsettin [Department of Physics, Faculty of Science and Arts, Gazi University, 06500 Ankara (Turkey)

    2010-04-15

    The effect of gamma-ray exposure on the metal-insulator-semiconductor (MIS) structures has been investigated using the electrical characteristics at room temperature. The MIS structures are irradiated with {sup 60}Co gamma-ray source. The energy distribution of interface states was determined from the forward bias I-V characteristics by taking into account the bias dependence of the effective barrier height and ideality factor. The value of series resistance decreases with increasing dose. Experimental results confirmed that gamma-ray irradiation have a significant effect on electrical characteristics of MIS structures.

  2. Effect of acute sup(60)Co-gamma-irradiation on the in vivo lipid peroxidation in experimental animals

    International Nuclear Information System (INIS)

    Ronai, Eva; Benkoe, Gy.

    1984-01-01

    The effect of sublethal (6.0 Gy) and LDsub(50/30) (9.0 Gy) doses of sup(60)Co-gamma-irradiation on malondialdehyde (MDA) level was studied in rats. The findings suggest that in the organs investigated (brain, liver, spleen, kidneys, testicles, stomach, small intestines) acute sup(60)Co gamma-irradiation increased the formation of MDA, the main product of lipid peroxidation, in a time-related manner to an extent characteristic of the organ investigated. Differences in the degree and temporal development of the changes allow some conclusions as to the radiosensitivity of individual organs. On this basis it can be assumed that the increase in MDA level caused by irradiation considerably contributes to the development of certain symptoms of radiation sickness. (author)

  3. Adhesion of thymus lymphocytes to aortic endothelial cells in rats irradiated with sup 60 Co-. gamma. -rays

    Energy Technology Data Exchange (ETDEWEB)

    Yongjian, Geng; Zijun, Mao; Zhiwei, Yin [Suzhou Medical Coll., JS (China)

    1990-03-01

    Adhesion of thymus lymphocytes to aortic endothelial cells in Wistar rats irradiated with {sup 60}Co-{gamma}-rays was preliminarily investigated with a stereological method. The results of experiments suggest that the number of lymphocytes of thymus which adhered to aortic endothelial cells significantly (p < 0.01) decreased after irradiation at doses of 2 and 8 Gy. However, when both thymus and aorta were irradiated, there were more lymphocytes adhering to endothelial cells than that when only thymus was irradiated.

  4. A study on the effect of 60Co gamma ray irradiation on the abrasion of dental polymethylmethacrylate, (3)

    International Nuclear Information System (INIS)

    Kimura, Hiroshi

    1981-01-01

    This report intends to clarify the relationship between the total exposure dose and scratch resistance to the specimens SF, SH, MF and MH, giving coating treatments to P.M.M.A. (dental polymethylmethacrylate) and exposing to the irradiation of 60 Co gamma ray at each dose rate. And based on the results, it is intended to develop coated P.M.M.A. with excellent scratch resistance give by irradiation of radioactive ray. From this study, the following results have been obtained. Irradiation of 60 Co gamma ray would give the best results at the exposure at 1 x 10 6 R. The SF and SH specimens in wet condition exposed to 60 Co gamma ray irradiation at 1 x 10 6 R showed a quantity of abrasion of only 17% that of untreated P.M.M.A. and the barrel test revealed outstanding abrasion and scratch resistance. Abrasion and scratch resistance of coated specimens are better utilized in wet conditions performing three times better than those in dry conditions. (author)

  5. Cytogenetic comparison of the responses of mouse and human peripheral blood lymphocytes to 60Co gamma radiation

    International Nuclear Information System (INIS)

    Kligerman, A.D.; Halperin, E.C.; Erexson, G.L.; Honore, G.; Westbrook-Collins, B.; Allen, J.W.

    1988-01-01

    Experiments were conducted to compare the chromosome damaging effects of 60 Co gamma radiation on mouse and human peripheral blood lymphocytes (PBLs). Either whole blood or isolated and pelleted mononuclear leucocytes (MNLs) were irradiated with a 60 Co unit to yield exposures of 1, 2, 3, or 4 Gy. In addition, mice were whole-body irradiated in vivo with the same doses so that an in vitro-in vivo comparison could be made. The results indicate that mouse PBLs irradiated in whole blood, whether in vivo or in vitro, respond similarly to 60 Co gamma rays as measured by dicentric chromosome formation. In addition, mouse and human PBLs showed a similar radiosensitivity, but because the mouse PBL data were best fitted to an exponential function and the human PBL data to a quadratic function, direct comparisons were difficult to make. Pelleted MNLs from mice were much less sensitive to the clastogenic effects of gamma radiation than whole blood. This is believed to be due to hypoxic conditions that developed during irradiation and transport. Human PBLs did not show a marked difference whether irradiated in whole blood or as pelleted MNLs in tissue culture medium

  6. Effects of gamma radiation from 60Co on dilute aqueous solutions of Linear Alkyl Sulfonate Surfactants and other organic pollutants

    International Nuclear Information System (INIS)

    Rohrer, D.M.

    1975-01-01

    This study is the result of research findings and operational experiences gained by the author in over four years of work associated with the use of 60 Co for the treatment of waste-water. The effects of 60 Co are discussed with regard to radiochemical destruction of specific organic pollutant species. The study deals specifically with the effects of gamma radiation from a 30,000 Ci 60 Co source upon aqueous solutions of Linear Alkyl Sulfonate Surfactants. The new Linear Alkyl Sulfonate (LAS) Surfactants, the major surfactant produced in the United States of America since June 1965, was developed to replace the old Alkyl Benzene Sulfonate (ABS) Surfactants. The reason for the removal of Alkyl Benzene Sulfonate Surfactants was their extreme environmental stability and the associated appearance of foam in waste-water treatment plants and receiving streams. Although the Linear Alkyl Sulfonate Surfactants are considered 'bio-degradable', the time required for 'bio-degradation' is impractical within the present environmental guidelines. This led to research into alternate techniques of treatment for the destruction of Linear Alkyl Sulfonate Surfactants. Consideration is also given to similar effects of gamma radiation upon pesticides and to the practical aspects of the use of gamma radiation for the treatment of waste-water. Included are discussions of the general experimental procedures used, the sources and their calibration, and sampling techniques to ensure the accuracy of the data. (author)

  7. Measurement of high dose rates of 60Co by gamma activation of115In and 111Cd foils

    International Nuclear Information System (INIS)

    Haddad, Kh; Qattan, M.; Taleb, A.

    2009-12-01

    The high gamma dose rate measurement technique using nuclear reaction (γ,(γ ' ') was introduced in this work. This technique is cheap, easy, reliable, and independent of chemical and physical factors, which affect other techniques. The response to the absorbed dose in this technique is linear and can be used for high dose. Cd and In foils were irradiated using 60 Co source and the resulted isomer activities were measured using gamma spectrometer. These foils were calibrated to be used as dosemeter and its results were compared with conventional one. The dose distribution in the irradiation field was determined using In foils. (authors)

  8. Influence of 60Co gamma radiation on Zabrotes subfasciatus (Bohemann, 1833) (Coleoptera, Curculionidae) and beams Phaseolus vulgaris

    International Nuclear Information System (INIS)

    Moraes Rego, A.F. de; Rodrigues, Z.A.; Oliveira, M.L. de; Santana, M.D.

    1986-01-01

    Influence of gamma radiation on Zabrotes subfasciatus (Boh, 1833) (Coleoptera Bruchidae) and the beans Phaseolus vulgaris (L.). The effects of 60 CO gamma radiation, 50 Gy, on both Phaseolus vulgaris (L.) seedbeans and adults of Zabrotes subfasciatus were studied using the no free choise method. Radiation decreased insect fertility hence insect population and it damage loss of weight and germination of seedbeans. However, radiation resulted in abnamal seedlings, showing various degrees of morphological malformation, although there was no effect on germination rates or seedling vigor. (Author) [pt

  9. Dominant lethal effect of gamma radiation of 60Co in Biomphalaria glabrata (SAY, 1818)

    International Nuclear Information System (INIS)

    Tallarico, Lenita de Freitas

    2003-01-01

    Germ cell mutations are used in ecotoxicological studies as biomarkers of population effects and indicators of ecological changes. Biomphalaria glabrata, a freshwater mollusk, is a good experimental model for biomonitoring studies due to its biological characteristics and the ecological importance of this invertebrate group. The dominant lethal test was established in B. glabrata for the detection of germ cell mutations. Results with chemical mutagens showed that this system is efficient, specific and sensitive in the evaluation of germ cell mutations induced by reference mutagens. In this work, the dominant lethal effects of gamma radiation of 60 Co were studied. A preliminary experiment was done to establish the dose range and to estimate the chronology of spermatogenesis in B. glabrata. This estimate is possible because of the uniformity in response to ionizing radiation between germ cells at homologous stages of spermatogenesis in widely different species. In general, pre-meiotic germ cells are less sensitive to the induction of lethal dominant mutations than post-meiotic cells. This effect can be attributed to: young gametogenic cells - mitotically active - have greater repair ability from sub-lethal DNA damage and there is a selective elimination of the damaged cells. In our system: induction of lethal dominant mutations causes an increase in the frequency of malformations and, cytotoxic effect is displayed as a reduction in the crossing rates. Total duration of spermatogenesis was estimated in approximately 36 days, with the following distribution of stages: 1 to 13 days - spermatogonia, 14 to 20 days - spermatocytes, 21 to 36 days - spermatids and spermatozoa. Based on this chronology, irradiated wild-type snails with 2,5; 10 and 20Gy and crossed with non-irradiated albino snails after 7, 17, 23, 30 and 36 days. The frequencies of malformations in the heterozygous wild-type offspring of the nonirradiated albino snails were used as indicator of germ cell

  10. Study on the energy dependence of gamma radiation detectors for {sup 137}Cs and {sup 60}Co; Estudo da dependencia energetica de detectores de radiacao gama para {sup 137}Cs e {sup 60}Co

    Energy Technology Data Exchange (ETDEWEB)

    Nonato, Fernanda B.C.; Diniz, Raphael E.; Carvalho, Valdir S.; Vivolo, Vitor; Caldas, Linda V.E., E-mail: fbnonato@ipen.b, E-mail: rediniz@ipen.b, E-mail: vcsouza@ipen.b, E-mail: vivolo@ipen.b, E-mail: lcaldas@ipen.b [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)

    2009-07-01

    38 Geiger-Mueller radiation detectors and 9 ionization chambers were calibrated, viewing to study the energy dependence of the monitor response for gamma radiation fields ({sup 137}Cs and {sup 60}Co). The results were considered satisfactory only for ionization chambers and for some Geiger-Mueller detectors

  11. Elimination of N-nitrosamines and their precusors in some foods by /sup 60/Co. gamma. -ray irradiation

    Energy Technology Data Exchange (ETDEWEB)

    Jifan, Hu; Yingqiang, He; Puju, Song

    1987-10-01

    N-Dimethylnitrosamine (NDMA) in some marine foods, such as dried shrimp, fish meal and salted fish, was drastically reduced by ..gamma..-ray irradiation with /sup 60/Co. The shrimp contained 54.6 ppb NDMA, but only 29.3, 8.8, 11.4, and 1.9 ppb were detected after irradiation with doses of 5, 10, 15 and 20 kGy. The corresponding reduction was 46.3%, 83.9%, 79.1%, and 96.6%, respectively. Similar results were also obtained in other two kinds of foods. NDMA could be completely eliminated by the irradiation in aqueous solution, which was much more markable than that in food samples. The major irradiated products of NDMA were dimethylamine and nitrous acid, but no re-formation of nitrosamines took place. /sup 60/Co irradiation also significantly decreased the content of nitrite in salted fish and in aqueous solution. No effect on nitrate could be observed.

  12. Effect of 60Co-gamma radiation on the properties of furs

    International Nuclear Information System (INIS)

    Raina, R.K.; Wali, B.K.; Wani, A.M.

    1990-01-01

    Furs pretanned with various combinations of vegetable tanning agents and retanned with alum have been irradiated with 60 Co γ-radiation in the dose range 5.0-114.0 kGy. The physico-chemical modifications induced by the radiation have been assessed by measuring changes in tensile strength, absorption of water, elongation and shrinkage temperature. For investigations, samples have been taken from the same topographic region of the rabbit furs, belonging to the same age and sex. The results are discussed. (author)

  13. Effect of 60Co-gamma radiation on the properties of furs

    Science.gov (United States)

    Raina, R. K.; Wali, B. K.; Wani, A. M.

    Furs pretanned with various combinations of vegetable tanning agents and retanned with alum have been irradiated with 60Co γ-radiation in the dose range 5.0-114.0 kGy. The physico-chemical modifications induced by the radiation have been assessed by measuring changes in tensile strength, absorption of water, elongation and shrinkage temperature. For investigations, samples have been taken from the same topographic region of the rabbit furs, belonging to the same age and sex. The results are discussed hereunder.

  14. Effects of 60Co gamma radiation on Biomphalaria glabrata (Say, 1818) Embryo, I. Mortality

    International Nuclear Information System (INIS)

    Okazaki, K.; Kawano, T.

    1990-01-01

    A study was conducted on the radiosensitivity of Biomphalaria glabrata embryos submitted to doses of 5, 10,15,20 and 25 Gy of 60 Co during the cleavage, blastula, gastrula, young trochophore and trochophore stages. Mortality was the parameter used to evaluated the damage induced by ionizing radiation. Susceptibility decreased with increasing embryo age and with decreased radiation dose. Estimated LD 50 values (15 days) showed that the cleavage stage (4.3 Gy) was approximately four times more radiosensitive than the trochophore stage (17.0 Gy). The survival curves obtained for each embryo stage are discussed on the basis of the multitarget theory. (author) [pt

  15. Effects on artificial blood substitute (Fluosol-DA) by 60Co gamma-ray irradiation

    International Nuclear Information System (INIS)

    Hishikawa-Itoh, Youko; Ayakawa, Yoshio; Miyata, Nobuki

    1982-01-01

    It had been demonstrated the high oxygen solubility of perfluorochemical emulsion (Fluosol-DA), and Fluosol-DA is going to apply as an oxygen carrier, namely artificial blood substitutes for clinical application. There have been many reports on physical, chemical and biological aspects of Fluosol-DA, but not have been reported on irradiation effects. In this paper, we investigated the effects on Fluosol-DA by 60 Co γ-ray irradiation. 1. pH of Fluosol-DA was increased apparently, this effect was not by irradiation, but by thermal effect. 2. Optical density of Fluosol-DA at 620 nm which meaned an effect on particles of Fluosol-DA was not changed by γ-irradiation. 3. F - ion concentration of Fluosol-DA which meaned an effect on structure of perfluorochemical was not changed merkedly. 4. The solubility of oxygen of Fluosol-DA was slightly increased by γ-irradiation at 25 0 C and 11 0 C, but this effect was disregarded. These results suggested that 60 Co γ-irradiation did not effect on pH, size or aggregation of particles, release of F - ion from Fluosol-DA and solubility of oxygen of Fluosol-DA. (author)

  16. Calibration of intense 60Co gamma ray sources at the Savannah River Plant

    International Nuclear Information System (INIS)

    Bibler, N.E.

    1976-05-01

    Three different dosimeters were used to calibrate Savannah River Plant 60 Co sources having intensities greater than 10 7 rads/hr. These dosimeters are (a) ceric sulfate dissolved in 0.4M H 2 SO 4 , (b) oxalic acid dissolved in water, and (c) a commercially available nylon film containing a radiochromic dye. Response per unit dose to these dosimeters is independent of radiation intensity at 10 4 to 10 11 rads/hr. The dosimeters were calibrated at 6.0 x 10 5 rads/hr with a 60 Co source whose intensity was determined with the standard Fricke dosimeter. For the sources at 10 7 rads/hr or greater, intensities were calculated from slopes of linear plots of dosimeter response versus irradiation time. Individual dose rates varied from 1.0 x 10 7 to 4.6 x 10 7 rads/hr. Each source was calibrated with at least two different dosimeters. Relative standard deviations varied from 2 to 9 percent. A conservative estimate of the uncertainty in the accuracy of these dosimeters is 10 percent. Of the three dosimeters, the nylon film is easiest to use and is therefore recommended for future calibrations

  17. Multistep process of neoplastic transformation of normal human fibroblasts by 60Co gamma rays and Harvey sarcoma viruses

    Energy Technology Data Exchange (ETDEWEB)

    Namba, M.; Nishitani, K.; Fukushima, F.; Kimoto, T.; Nose, K.

    1986-03-15

    As reported previously (Namba et al., 1985), normal human fibroblasts were transformed by 60Co gamma-ray irradiation into immortal cells with abnormal karyotypes. These transformed cells (KMST-6), however, showed a low cloning efficiency in soft agar and no transplantability. However, upon treatment with Harvey murine sarcoma virus (Ha-MSV), the cells acquired elevated clonability in soft agar and transplantability in nude mice. Ha-MSV alone, however, did not convert normal human fibroblasts into either immortal or tumorigenic cells. The Ha-MSV-transformed KMST-6 cells showed an enhanced expression of the ras oncogene, but normal and 60Co gamma-ray-transformed cells did not. Our current data suggest that gamma rays worked against normal human cells as an initiator, giving rise to chromosome aberrations and immortality, and that Ha-MSV, probably through its ras oncogene, played a role in the progression of the malignant cell population to a more malignant one showing enhanced colony formation in soft agar and tumorigenicity in nude mice.

  18. INCASoy-36: soybean variety obtained in Cuba after the induction of mutations with 60Co gamma rays

    International Nuclear Information System (INIS)

    Ortiz, R; FeII, C de la; Ponce M

    2008-01-01

    INCASoy-36 variety was obtained after irradiating seeds from INCASoy-15 at doses of 240 Gy 60 Co gamma rays. This variety is adapted to summer and winter seedings; however, it can also be used in spring. Besides, its yields can reach up to 3,5-4,0 t.ha-1 grains in summer and spring seedings. It is tolerant to the main pests and diseases; it is especially resistant to Meloidogyne incognita attacks. During the adverse spring season, seeds have been damage tolerant. It can assure high productions with low inputs; therefore, it is feasible to Cuban agriculture. (Author)

  19. Effect of. gamma. -radiation of /sup 60/Co on bacteria Sphaerotilus natans in nutrient medium containing calcium and lithium

    Energy Technology Data Exchange (ETDEWEB)

    Leonowicz-Babiak, K; Radziwolska, E; Widera, M

    1980-01-01

    The effect of ..gamma..-radiation of /sup 60/Co on an increase in dry matter of the polysaprobic zone bacteria Sphaerotilus natans was studied. Bacterial growth depended on the radiation dose absorbed. With a rise of the dose, the bacterial dry matter usually decreased. This relationship was not observed at a radiation dose of 72 krad. In the presence of calcium in the medium, the bacterial dry matter increased. An addition of lithium salt to the medium failed to increase the bacterial dry matter.

  20. Investigations of immunoglobulins, circulating immune complexes and plasma free hemoglobin in cancer patients on 60Co gamma-ray therapy

    International Nuclear Information System (INIS)

    Horvath, M.; Rode, I.L.; Fekete, B.; Kiss, B.; Ringwald, G.

    1981-01-01

    32 patients with different tumours were irradiated by 60 Co gamma-rays. During therapy lasting for several weeks, changes in the content of immunoglobulin and of some other serum proteins, circulating immune complexes and plasma free hemoglobin were determined. Immunosuppression according to immunoglobulin content in serum was not produced by this type of radiation. Decrease in immune complex levels was a good prognostic sign. Low values of plasma hemoglobin content during treatment indicated that no erythrocyte membrane damage had been effected. (orig.) [de

  1. Long-term follow-up after accidental gamma irradiation from a 60Co source

    International Nuclear Information System (INIS)

    Klener, V.; Tuscany, R.; Vejlupkova, J.; Dvorak, J.; Vlkovic, P.

    1986-01-01

    In December 1973 a technician was accidentally irradiated when attempting to bring under control a sealed 60 Co source (110 TBq) which had been lodged in the head of a medical irradiation unit during a replacement operation. In the early period after the accident, severe skin changes on the left hand, epilation in a small area of the left temporal region and minor deviations in peripheral blood developed. In the following years, repeated surgery due to secondary skin defects of the left hand resulted in the loss of the fingers 2-5. Since 1975, changes in the lens of the left eye began to appear leading gradually to the deterioration of visual acuity. Later, opacities of the lens of the right eye were found. The patient's psychological and emotional attitude about the accident changed in the course of time. The factors influencing the psychic state of the patient are identified

  2. Determination of high level absorbed dose in a 60Co gamma ray field with ionization chambers

    International Nuclear Information System (INIS)

    Zhongying Li; Benjiang Mao; Lu Zhang

    1995-01-01

    This paper relates to the principles and methods for determining the absorbed dose of high energy photons radiation with ionization chambers, and its shows the doserate results of high level 60 Co γ-rays in water measured with Farmer chambers. The results with two kinds of chambers at a same point are consistent within 0.3%, and the total uncertainty is less than ± 4%. In the domestic intercomparison on determining high level absorbed dose in which 12 laboratories participated, the deviation of our result from the mean result of the intercomparison is -0.04% [Chen Yundong (1992). Summing up report on a high level absorbed dose intercomparison (in Chinese)]. (author)

  3. Influence of beta radiation from tritium and gamma radiation from 60Co on the biological half-times of organically bound tritium

    International Nuclear Information System (INIS)

    Radwan, I.

    1981-01-01

    The influence of beta radiation from tritium on the biological half-times of organically bound tritium in particular tissues of the rat is compred with the influence of fractionated gamma radiation from 60 Co. (M.F.W.)

  4. Deep defect levels in standard and oxygen enriched silicon detectors before and after **6**0Co-gamma-irradiation

    CERN Document Server

    Stahl, J; Lindström, G; Pintilie, I

    2003-01-01

    Capacitance Deep Level Transient Spectroscopy (C-DLTS) measurements have been performed on standard and oxygen-doped silicon detectors manufactured from high-resistivity n-type float zone material with left angle bracket 111 right angle bracket and left angle bracket 100 right angle bracket orientation. Three different oxygen concentrations were achieved by the so-called diffusion oxygenated float zone (DOFZ) process initiated by the CERN-RD48 (ROSE) collaboration. Before the irradiation a material characterization has been performed. In contrast to radiation damage by neutrons or high- energy charged hadrons, were the bulk damage is dominated by a mixture of clusters and point defects, the bulk damage caused by **6**0Co-gamma-radiation is only due to the introduction of point defects. The dominant electrically active defects which have been detected after **6**0Co-gamma-irradiation by C-DLTS are the electron traps VO//i, C//iC//s, V//2( = /-), V //2(-/0) and the hole trap C//i O//i. The main difference betwe...

  5. Effect of Zi Gui decoction on immune function in {sup 60}Co {gamma}-ray irradiated mice

    Energy Technology Data Exchange (ETDEWEB)

    Qiujun, Lu; Shafei, Huang; Xipeng, Zhou; Jiayun, Song; Zhongxiong, Tang [Dept. of Pharmacology, Institute of Radiation Medicine, Beijing (China)

    1995-02-01

    Zi Gui decoction (ZG), a complex preparation of traditional Chinese herbal medicine, mainly consists of Radix Angelicae and Radix Astragali. The effects of ZG on mitogen induced proliferation IL-1 and -2 production, natural killer (NK) cell activity in {sup 60}Co {gamma}-ray irradiated mice is investigated. After 5 Gy whole body irradiation. mice were treated i.g. with ZG (1.2, 1.8 g/kg/day) for 20 days. An enhancement in Con A- and LPS-induced proliferations of splenocytes from the two dosage groups were observed. There were marked increases in IL-1 activity in peritoneal macrophage culturesa and IL-2 activity in splenocyte cultures from irradiated mice treated with ZG. The two dosage groups also showed significant potentiation of NK cell activity against YAC-1 target cells. The above results indicated that ZG could promote the recovery of immune functions in {gamma}-ray irradiated mice.

  6. Effects of 2.0 Gy of 60Co gamma rays irradiation on rat embryos

    International Nuclear Information System (INIS)

    Lee, Juing-Yi; Satow, Yukio

    1987-01-01

    Pregnant rats of Donryu strain were exposed to a whole-body 60 Co γ ray irradiation of a single dose of 2.0 Gy (Dose rate: 0.5 Gy/min) on day 7, 8, 9, 10 or 11 of gestation (sperm day = day 0). The rats were sacrificed on day 18 and the offspring were examined for external and visceral malformations. Malformed embryos occurred between days 7 and 11 with the highest incidence occurring on day 9. Dose with 2.0 Gy increased the rate of resorption or death (52.1 %), in the survivors, caused congenital malformation in a majority of embryos (86.5 %) on day 8 of gestation. There is an increase in malformation (93.3 %) and growth retardation, but no increase in mortality (42.9 %) on day 9 of gestation. Relatively few anomalies resulted from irradiation on day 7 of gestation. The peak day for cardiovascular anomalies occurred on day 9 (88.3 % of all survival embryos) with high levels also occurring on day 8 (86.5 %). Cardiovascular anomalies consisted of VSD, hypoplasia of the pulmonary trunk, coarctation of the aorta, double aortic arch, right aortic arch, riding aorta, complete transposition of the aorta, persistent atrioventricular canal, vascular ring, aberrant right subclavian artery and others. Similar anomalies, but at a lower incidence, were produced by 60 Co γ ray at dose levels of 2.0 Gy on day 10 or 11 of gestation. Cases of cleft lip and cleft palate or facial cleft were observed seventeen fetuses on day 9 of gestation (31 %). Exencephaly occurred in nine embryos treated on day 9 (16.1 %) and in one embryos treated on day 10. Tail defects appeared with treatment on day 9 with the latter predominating on day 11. The present study show that maximum resorption (52.1 %) was seen with treatment on day 8 whereas the highest rate of malformation (93.3 %) was observed with treatment on day 9. (J.P.N.)

  7. Use of 60Co gamma radiation in increased levels of total polyphenol extracts of bark of Schinus terebinthifolius Raddi

    International Nuclear Information System (INIS)

    Santos, Gustavo H.F.; Silva, Edvane B.; Silva, Hianna A.M.F.; Amorin, Elba L.C.; Peixoto, Tadeu J.S.; Yara, Ricardo; Lima, Claudia S.A.

    2013-01-01

    Schinus terebinthifolius Raddi (Anacardiaceae) is well known as sources of phenolic compounds. Known as mastic pepper, red pepper tree is a plant native to midsize coast of Brazil. Some of its structures have proven antibacterial, anti-inflammatory, antifungal and healing. The aim of this study was to evaluate the difference in the phenol contents of crude extracts that were measured after irradiating the barks of S. terebinthifolius using gamma radiation from 60 Co. The crude extract were divided into a control group and eight experimental groups, which were separated based on the doses of gamma radiation to which they were exposed: 2.5, 5.0, 7.5, 10.0, 12.5, 15.0, 20.0 and 50.0 kGy (Assays were performed in triplicate). The results allow observe that gamma radiation promoted in extracts of bark of S. terebinthifolius, many percents increase (p> 0.05) of total polyphenol content between 2.5 kGy (41.93%) and 50.0 kGy (44.52%) compared to 0 kGy (30.07%), with the same gradual to 10.0 kGy, and reaching peak maximum at 10.0 kGy (68.44%). However, the study puts the process of gamma radiation from 60 Co as an alternative significant increase in the percentage of some natural substances of plant material, and subsequently contribute to the augmentation of various therapeutic applications to which they are assigned. (author)

  8. Use of {sup 60}Co gamma radiation in increased levels of total polyphenol extracts of bark of Schinus terebinthifolius Raddi

    Energy Technology Data Exchange (ETDEWEB)

    Santos, Gustavo H.F.; Silva, Edvane B.; Silva, Hianna A.M.F.; Amorin, Elba L.C.; Peixoto, Tadeu J.S.; Yara, Ricardo; Lima, Claudia S.A., E-mail: santosghf@hotmail.com, E-mail: edvborges@yahoo.com, E-mail: amdemelo@hotmail.com, E-mail: claudia.salima@gmail.com, E-mail: ricardo.yara@gmail.com, E-mail: tadeu1903@yahoo.com.br, E-mail: elba@ufpe.br [Universidade Federal de Pernambuco (UFPE), Recife, PE (Brazil)

    2013-07-01

    Schinus terebinthifolius Raddi (Anacardiaceae) is well known as sources of phenolic compounds. Known as mastic pepper, red pepper tree is a plant native to midsize coast of Brazil. Some of its structures have proven antibacterial, anti-inflammatory, antifungal and healing. The aim of this study was to evaluate the difference in the phenol contents of crude extracts that were measured after irradiating the barks of S. terebinthifolius using gamma radiation from {sup 60}Co. The crude extract were divided into a control group and eight experimental groups, which were separated based on the doses of gamma radiation to which they were exposed: 2.5, 5.0, 7.5, 10.0, 12.5, 15.0, 20.0 and 50.0 kGy (Assays were performed in triplicate). The results allow observe that gamma radiation promoted in extracts of bark of S. terebinthifolius, many percents increase (p> 0.05) of total polyphenol content between 2.5 kGy (41.93%) and 50.0 kGy (44.52%) compared to 0 kGy (30.07%), with the same gradual to 10.0 kGy, and reaching peak maximum at 10.0 kGy (68.44%). However, the study puts the process of gamma radiation from {sup 60}Co as an alternative significant increase in the percentage of some natural substances of plant material, and subsequently contribute to the augmentation of various therapeutic applications to which they are assigned. (author)

  9. Evaluation of gamma-sterilization (60Co) by RT-PCR by DHFR expression detection

    International Nuclear Information System (INIS)

    Converso, Ana Paula G.; Andrade Junior, Heitor F. de; Vieira, Daniel P.

    2007-01-01

    The improvement of techniques to detect pathogen agents in blood had reduced significantly the contamination mechanisms by hemocomponents in blood transfusion procedures. Ionizing radiation is a method that has presented several applications on medicine and in currently days has been showing special attention on blood banks which has been applied to avoid TA-GVHD development. DHFR is an enzyme constitutive in Plasmodium protozoa and has an important role in folate metabolism on these parasites. Detecting the expression of RNAm coder for this enzyme is possible to evaluate the viability of this parasite in blood samples. Plasmodium chabaudi AJ is a parasite that induces lethal malaria in rodents similar to human malaria In this work, the objective was to detect the presence of plasmodium protozoa in irradiated blood samples, infected experimentally, through the application of a RT-PCR using primers for the coder sequence of DHFR's mRNA. We studied doses of ionizing radiation between 0 and 75 Gy. The irradiation procedures were accomplished in Center of Radiation Technology of IPEN-CNEN in a 60 Co panoramic source. Our results had demonstrated that RT-PCR is a sensible method to evaluate the viability of plasmodium in blood samples because the technique could detect low parasite burden in all tested samples. (author)

  10. Evaluation of gamma-sterilization ({sup 60}Co) by RT-PCR by DHFR expression detection

    Energy Technology Data Exchange (ETDEWEB)

    Converso, Ana Paula G.; Andrade Junior, Heitor F. de [Instituto de Medicina Tropical de Sao Paulo, Sao Paulo, SP (Brazil)]. E-mail: anapaulagconverso@gmail.com; hfandrad@usp.br; Vieira, Daniel P. [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil); Instituto de Medicina Tropical de Sao Paulo, Sao Paulo, SP (Brazil); E-mail: dperezv@usp.br

    2007-07-01

    The improvement of techniques to detect pathogen agents in blood had reduced significantly the contamination mechanisms by hemocomponents in blood transfusion procedures. Ionizing radiation is a method that has presented several applications on medicine and in currently days has been showing special attention on blood banks which has been applied to avoid TA-GVHD development. DHFR is an enzyme constitutive in Plasmodium protozoa and has an important role in folate metabolism on these parasites. Detecting the expression of RNAm coder for this enzyme is possible to evaluate the viability of this parasite in blood samples. Plasmodium chabaudi AJ is a parasite that induces lethal malaria in rodents similar to human malaria In this work, the objective was to detect the presence of plasmodium protozoa in irradiated blood samples, infected experimentally, through the application of a RT-PCR using primers for the coder sequence of DHFR's mRNA. We studied doses of ionizing radiation between 0 and 75 Gy. The irradiation procedures were accomplished in Center of Radiation Technology of IPEN-CNEN in a {sup 60}Co panoramic source. Our results had demonstrated that RT-PCR is a sensible method to evaluate the viability of plasmodium in blood samples because the technique could detect low parasite burden in all tested samples. (author)

  11. Immune response against antigens irradiated with 60Co gamma-rays

    International Nuclear Information System (INIS)

    Baptista, J.A.; Spencer, P.J.; Oliveira, J.E.; Casare, M.S.; Nascimento, N.

    2006-01-01

    In the present work, we investigated the immunological behavior of bothropstoxin-1, a K49 phospholipase from Bothrops jararacussu, and of ovalbumin before and after irradiation with 60 Co γ-rays. Isogenic mice were immunized with either native or irradiated proteins. The circulating antibodies were isotyped and titrated by ELISA. Results indicate that irradiated proteins were immunogenic and the antibodies elicited by them were able to recognize the native proteins in ELISA. Data also indicate that the irradiated protein induced higher titers of IgG2a and IgG2b, suggesting that Th1 cells were predominantly involved in the immune response. Structural modifications of the proteins were investigated by SDSPAGE, mass spectrometry and size exclusion chromatography. According to our data, irradiation promoted structural modifications on both proteins, characterized by higher molecular weight forms (aggregates and oligomers). When analyzed by mass spectrometry, the irradiated bothropstoxin appeared in several oxidized forms. These results indicate that irradiation of toxic proteins can promote significant modifications in their structures, but still retain many of the original antigenic and immunological properties of native form. (author)

  12. Effects of 60Co gamma radiation on defense function of human polymorphonuclear leukocytes

    International Nuclear Information System (INIS)

    Sasagawa, Sumiko; Suzuki, Kazuo; Sakatani, Tatsuichiro; Brooks, G.T.; Fujikura, Toshio.

    1986-06-01

    The effects of radiation on defense function of polymorphonuclear leukocytes (PMN) were studied following irradiation with 60 Co γ radiation (30 - 3,000 rad) using PMN separated from the peripheral blood of healthy volunteers. The migration distances for all three measures of chemotaxis to fMet-Leu-Phe (10 -8 M), chemokinesis induced by fMet-Leu-Phe, and random migration tended to decrease with increasing dose, showing 0.0054 μm/rad (p -5 M) in conjunction with cytochalasin B (CB, 5 μg/ml) there was a significant dose trend, showing the dose effects of decreasing 0.0022 % release/rad for BGL and 0.0030 % release/rad for LYZ with increasing dose. In superoxide anion (O 2 - ) production, a slight and marginally significant linear dose trend was found. These results suggest that the defense function of PMN is not so resistant to radiation as predicted from the fact that PMN in the peripheral blood are differentiated and mature. It is thought that radiation inflicts substantially harmful effects on the defense function of peripheral PMN. (author)

  13. Chemical changes in the chloroform-paraffin system irradiated by 60Co gamma-rays, 1

    International Nuclear Information System (INIS)

    Yoshino, Tomio; Sakaue, Muneko; Shimizu, Yasuko; Kawamura, Fumio.

    1979-01-01

    It has been reported that the chloroform-paraffin-dye system have excellent sensitivity for radiation as a solid chemical dosimeter or a phantom. However, the chemical changes in the irradiated system are not examined in detail. In the present study, the effect of paraffin on changes in the above system of a liquid state irradiated by 60 Co γ-rays was examined by using various normal paraffin, and the other variable factors on the changes were done. When the chloroform solution and the solution containing 25 per cent of paraffin by volume with 5.0 x 15 -5 mol/liter of Methyl Yellow as a dye were irradiated by 2000 R, G values for the formation of hydrogen chloride in the both solutions were 8.4 and 10.8, respectively, and were little affected by the kind of those, from C 6 (hexane) to C 36 (hexatria-contane). These results suggest that chlorine radical formed by radiolysis of chloroform may react with hydrogen atom from paraffin, thereby increasing the amount of hydrogen chloride. Presence of oxygen increased G value of the chloroform solution from 7.6 to 8.4, but did little that of the solution containing paraffin. (author)

  14. Effect of 60 Co gamma radiation on trehalose level of Saccharomyces boulardii cells

    International Nuclear Information System (INIS)

    Santos, Raquel Gouvea dos; Neves, Maria Jose; Duarte, Rinaldo; Nicoli, Jacques R.

    1996-01-01

    The medicine Floratil used for diarrhea treatment is composed by lyophilized yeast cells of Saccharomyces boulardii. The action mechanism of this product is unknown. The efficacy of S. boulardii depends on its viability. The increase in the trehalose level in S. cerevisiae cells submitted to a previous sub-lethal level heat shock results in resistance to a lethal shock. The aim of this work was to study weather the cell trehalose level is involved also in the resistance of gamma irradiation. It was noted that the yeast cells surviving to gamma irradiation had more trehalose level than control cells. Apparently there is a positive correlation between of trehalose level and gamma irradiation resistance of S.boulardii cells. (author)

  15. Morphological and histological studies on freshwater prawn Macrobrachium rosenbergii (de man) irradiated with {sup 60}Co gamma radiation

    Energy Technology Data Exchange (ETDEWEB)

    Stalin, A.; Broos, K.V. [P.G. and Research Department of Zoology, Jamal Mohamed College (Autonomous), Tiruchirappalli 620020, Tamil Nadu (India); Sadiq Bukhari, A., E-mail: abjmc@yahoo.in [P.G. and Research Department of Zoology, Jamal Mohamed College (Autonomous), Tiruchirappalli 620020, Tamil Nadu (India); Syed Mohamed, H.E. [P.G. and Research Department of Zoology, Jamal Mohamed College (Autonomous), Tiruchirappalli 620020, Tamil Nadu (India); Singhal, R.K. [Analytical Spectroscopy Section, Analytical Chemistry Division, Bhabha Atomic Research Center, Trombay, Mumbai 4000085 (India); Venu-Babu, P. [Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Center, Trombay, Mumbai 4000085 (India)

    2013-11-15

    Graphical abstract: -- Highlights: •Analyzing the impact of {sup 60}Co gamma radiation (low doses) in M. rosenbergii. •LD{sub 50} identified in 30 Gy; Irradiated exhibits morphological changes than control. •Significantly decreased HSI and histological changes in irradiated groups. •Aberration severities increased as increased of dose level. •Biomonitoring tool in determining low dose radiation pollution in aquatic ecosystem. -- Abstract: This study was framed to investigate the {sup 60}Co gamma radiation induced morphological and histological variations in freshwater prawn Macrobrachium rosenbergii. The LD{sub 50} value of {sup 60}Co gamma irradiated M. rosenbergii observed (by probit analysis) at 30 Gy. Prawns were irradiated to four different dose levels (3 mGy, 30 mGy, 300 mGy and 3000 mGy) using Theratron Phoenix TeleCobalt Unit [P-33] and one control group (without irradiation) maintained separately. Irradiated groups exhibited several morphological variations such as discoloration; damaged rostrum; opaque coloration in cephalothorax; black bands and dot formation in abdomen; deformed uropods and telson in tail regions when compared with control group. The Hepato Somatic Index reflected the severity of radiation on hepatopancreas. Histological variations in gills, hepatopancreas and muscles of irradiated groups were observed. In gills, structural changes such as swollen and fused lamellae, abnormal gill tips, hyperplasic, necrotic and clavate-globate lamellae were observed in gamma irradiated prawns. Accumulation of hemocytes in hemocoelic space, interstitial sinuses filled with abnormal infiltrated hemocytes, the tubular epithelium with ruptured basal laminae, abnormal and coagulated lumen, necrotic tubules, thickened basal laminae, tissue debris, necrotic hepatocytes were observed in irradiated prawn hepatopancreas. In muscle, shrinkage of muscular fiber and necrotic musculature were observed in irradiated prawns. These structural alterations of

  16. Biological changes in Musca domestica L., 1758 (Diptera:Muscidae), induced by gamma radiation (60 Co)

    International Nuclear Information System (INIS)

    Grecchi, M.A.

    1989-01-01

    This work was carried out in the Entomology Section of the Centre of Nuclear Energy in Agriculture (CENA), University of Sao Paulo, Piracicaba, Sao Paulo, Brazil. The objective of the present research work was to investigate some effects of gamma radiation on the various stages of M. domestica life cycle. (author)

  17. Response of avocado genotypes to improvement through 60Co gamma radiation

    International Nuclear Information System (INIS)

    Cruz, E. De la; Rubi A, M.; Garcia A, J.M.

    1997-01-01

    Ten avocado genotypes were subjected to gamma radiation from 0 to 45 Gy in 1993. Vegetative and reproductive data were analysed in a factorial design. Genotypes differed significative on height and fruit number. Radiation affected significative fruit number but not tree height. ''Hass'' showed strongest interaction between genotype and doses, for fruit number. (Author)

  18. Effect of gamma irradiation (60CO) on quatitative characters of barley (Hordeum vulgare)

    International Nuclear Information System (INIS)

    Santana, T.C.; Gonzalez, F.C.

    1984-01-01

    Seeds od f a barley line were irradiated with doses ranging from O to 64 Kr of gamma radiation for three consecutive generations (R1,R2 and R3). From these, several mutant generations were obtained in the field, planting at a commercial density and without selection. (M.A.C.) [pt

  19. Evaluation of fruit productivity and quality in Hass avocado submitted to {sup 60}Co gamma radiation; Evaluacion de productividad y calidad de fruta en aguacate Hass sometido a radiacion gamma de {sup 60}Co

    Energy Technology Data Exchange (ETDEWEB)

    Cruz T, E. De la; Ibanez P, J; Mijares O, P; Garcia A, J M [Instituto nacional de Investigaciones Nucleares, Departamento de Biologia, A.P. 18-1027, 11801 Mexico D.F. (Mexico)

    1999-07-01

    Evaluation of productivity, postharvest behavior and fruit quality was performed on four years Hass avocado trees irradiated with {sup 60} Co gamma rays in doses of 5, 10, 15, 20 and 25 Gy, established in the ''La Labor'' Experimental Center of the Centro de Investigaciones Cientificas y Tecnologias del Aguacate en el Estado de Mexico (CICTAMEX) at Temascaltepec Mexico. Productivity had a significant increase in the dose of 15 Gy being the average number of fruits nearly 400 % more than the control at fruit setting, being such difference reduced at fruit harvesting to 300 %. In regard to postharvest performance, the respiration index (mg CO{sub 2}/kg/hr) did not show significant differences among treatments. Also others variables such as physiological weight losses, texture, maturity pattern, and sensorial tests (color, flavor, aroma, texture) were not different in regard to the control. This mean that radiation has altered productivity but not the quality and postharvest behavior of fruits. (Author)

  20. Efect of Gamma 60Co Irradiation on The Growth, Lipid Content and Fatty Acid Composition of Botryococcus sp. Microalgae

    Directory of Open Access Journals (Sweden)

    Dini Ermavitalini

    2017-04-01

    Full Text Available Botryococcus sp. is one of microalgae species that has a high lipid content as much as 75% of their dry weight. But, lipid production by microalgae is regulated by their environmental condition (pH, light, temperature, nutrition, etc. Mutagenesis induced by Gamma 60Co irradiation can be utilized to alter the Botryococcus sp. genetic to get microalgae mutant strain that can produce a higher lipid content than the wild strain. Botryococcus sp. was irradiated with different doses of gamma ray of 60Co  (0, 2, 4, 6, and 10 Gy,  and the effect  on the growth, lipid content, and fatty acid composition of microalgae were observed. Research design used is random complete (RAL with 95 %  confident level for quantitive analysis based on the biomass and lipid contents. More over fatty acid composition was analyzed by Gas Cromatography-Mass Spectrometry (GC-MS. Results showed that Gamma irradiated gave an effect on growth and lipid content of Botryococcus sp. But between the control treatment (0 Gy with microalgae irradiated dose of 2 Gy, 4 Gy and 6 Gy were not significantly different. Whereas between the control with 10 Gy irradiated was significantly different. The highest biomassa and lipid content are found in 10 Gy irradiated microalgae with 0.833 gram biomass and 41% lipid content. Fatty acid profile of Botryococcus sp. control has 6 fatty acids while 10 Gy irradiated microalgae has 12 fatty acids, with the long-chain fatty acids increased, whereas short-chain fatty acids decreased.

  1. Morphological and histological studies on freshwater prawn Macrobrachium rosenbergii (de man) irradiated with (60)Co gamma radiation.

    Science.gov (United States)

    Stalin, A; Broos, K V; Sadiq Bukhari, A; Syed Mohamed, H E; Singhal, R K; Venu-Babu, P

    2013-11-15

    This study was framed to investigate the (60)Co gamma radiation induced morphological and histological variations in freshwater prawn Macrobrachium rosenbergii. The LD50 value of (60)Co gamma irradiated M. rosenbergii observed (by probit analysis) at 30 Gy. Prawns were irradiated to four different dose levels (3 mGy, 30 mGy, 300 mGy and 3,000 mGy) using Theratron Phoenix TeleCobalt Unit [P-33] and one control group (without irradiation) maintained separately. Irradiated groups exhibited several morphological variations such as discoloration; damaged rostrum; opaque coloration in cephalothorax; black bands and dot formation in abdomen; deformed uropods and telson in tail regions when compared with control group. The Hepato Somatic Index reflected the severity of radiation on hepatopancreas. Histological variations in gills, hepatopancreas and muscles of irradiated groups were observed. In gills, structural changes such as swollen and fused lamellae, abnormal gill tips, hyperplasic, necrotic and clavate-globate lamellae were observed in gamma irradiated prawns. Accumulation of hemocytes in hemocoelic space, interstitial sinuses filled with abnormal infiltrated hemocytes, the tubular epithelium with ruptured basal laminae, abnormal and coagulated lumen, necrotic tubules, thickened basal laminae, tissue debris, necrotic hepatocytes were observed in irradiated prawn hepatopancreas. In muscle, shrinkage of muscular fiber and necrotic musculature were observed in irradiated prawns. These structural alterations of the organs it is felt could affect the vital physiological functions such as respiration, osmotic and ionic regulation in gills and muscles; absorption, storage and secretion of the hepatopancreas which in turn could adversely affect the growth and survival of freshwater prawn M. rosenbergii. Copyright © 2013 Elsevier B.V. All rights reserved.

  2. Influence of 60Co gamma radiation on fluorine plasma treated enhancement-mode high-electron-mobility transistor

    International Nuclear Information System (INIS)

    Quan Si; Hao Yue; Ma Xiao-Hua; Yu Hui-You

    2011-01-01

    AlGaN/GaN depletion-mode high-electron-mobility transistor (D-HEMT) and fluorine (F) plasma treated enhancement-mode high-electron-mobility transistor (E-HEMT) are exposed to 60 Co gamma radiation with a dose of 1.6 Mrad (Si). No degradation is observed in the performance of D-HEMT. However, the maximum transconductance of E-HEMT is increased after radiation. The 2DEG density and the mobility are calculated from the results of capacitance-voltage measurement. The electron mobility decreases after fluorine plasma treatment and recovers after radiation. Conductance measurements in a frequency range from 10 kHz to 1 MHz are used to characterize the trapping effects in the devices. A new type of trap is observed in the F plasma treated E-HEMT compared with the D-HEMT, but the density of the trap decreases by radiation. Fitting of G p /ω data yields the trap densities D T = (1 − 3) × 10 12 cm −2 · eV −1 and D T = (0.2 − 0.8) × 10 12 cm −2 · eV −1 before and after radiation, respectively. The time constant is 0.5 ms-6 ms. With F plasma treatment, the trap is introduced by etch damage and degrades the electronic mobility. After 60 Co gamma radiation, the etch damage decreases and the electron mobility is improved. The gamma radiation can recover the etch damage caused by F plasma treatment. (interdisciplinary physics and related areas of science and technology)

  3. Study on the viability of peach and apple pollen treated with gamma rays 60Co

    International Nuclear Information System (INIS)

    Filev, K.; Angelov, A.

    1976-01-01

    Pollen from Marygold peach and from Golden Delicious apple varieties was treated with gamma-rays at rates of 0.5, 1.5, 10.0, 50.0, 100, 200, 300, 400 and 500 kR, the irradiation intensity being 1350 r/min. Results point to a different influence of gamma-rays irradiation on the pollen germination. Apple pollen proved more radiosensitive. Least dose of o.5 kR exerted a stimulative effect in the pollen germination. As the irradiation dose rcse, the germination percentage declined. Doses of over 400 kR destroyed completely pollen viability in both fruit species. A linear semi-logarithmical dependence was ascertained between the irradiation rate and pollen germination. 5 and 10 kR proved most suitable for the purposes of the experimental mutagenesis at peach and apple pollen irradiation. (author)

  4. Evaluation of gamma radiation (60-Co) induced mutation in two Phaseolus vulgaris varieties

    International Nuclear Information System (INIS)

    Silva, R.M.

    1984-01-01

    Two varieties of Phaseolus vulgaris (Jutiapan and San Martin) were irradiated at 0, 8, 15, 20 and 30 kR doses in a 60-cobalt gamma source, to identify mutants and 20% lethality. M 2 plants showing morphogical mutations were selected. Differences in sensitivity to irradiation of the two varieties were noted, using data and physiological effects of M 1 . Selection and analysis for protein content were in M 3 as well as hereditary changes. (M.A.C.) [pt

  5. The induction mutation effects of "6"0Co gamma radiation on physiological growth of tomato

    International Nuclear Information System (INIS)

    Gusti Ngurah Sutapa; I Gde Antha Kasmawan

    2016-01-01

    Almost all types of cuisine in Indonesia are using tomatoes as the base material of manufacture. The nutritional value contained in tomatoes is also quite high, because there is a number of vitamin content required by the human body. In addition, the tomatoes in plants featured national horticultural commodity and priority on a number of provinces in Indonesia. So many benefits of tomatoes indicates that the productivity of tomatoes should be improved. One improvement in terms of quality can be done by means of mutation induction with gamma radiation of Co-60. Induction of mutations are genetic changes caused by human effort, one of them is by using radioactive materials. Gamma rays of Co-60 from the IRPASENA facility was exposed to tomato seeds at doses of 50, 100, 150, 200 and 250 Gy. And then measurements were conducted on the physiological growth of leaf width, plant height, number of fruit and wet weight of tomatoes from week 1 until harvest. The results showed a growth curve of tomato is in accordance with sigmoidal plant physiological growth curve. Optimal physiological growth of tomato plants was obtained at dose of gamma radiation of 100 Gy. At this optimal dose physiological growth of tomato plants is the best (superior) than in doses below and above 100 Gy and control. (author)

  6. Nutritional balance of pigs irradiated on the hind part with 1100 rd of 60Co gamma rays

    International Nuclear Information System (INIS)

    Daburon, F.; Tricaud, Y.; Bourhoven, D.; Marion, C.

    1978-01-01

    The hind part of 10 adult miniature pigs was irradiated with 1100 rd of 60 Co gamma rays. The dietary mineral (Na, K, Ca, P), nitrogen and lipid balances of the animals were studied before and 1, 3 and 5 months after irradiation. While the classical early lesion of the intestinal mucosa recovered quite satisfactorily within one or two months, a pancreatic atrophy process developed progressively which might play a major role in the nutritional state of the pigs and thus in their survival. Concerning nutritional balance, nitrogen and sodium retention were perturbed until 5 months post-irradiation; cyanocobalamin absorption remained very low. Dry and organic matter utilization recovered nearly normal values in the 3rd ou 5th month [fr

  7. Differential production of proteolytic enzymes by normal human fibroblasts and their counterparts transformed by treatment with 60Co gamma rays

    International Nuclear Information System (INIS)

    Nishitani, Koji; Namba, Masayoshi; Ohkubo, Shigeki; Kimoto, Tetsuo

    1985-01-01

    Production of proteolytic enzymes by human fibroblasts in the process of transformation was investigated. The cells used were normal human fibroblasts (KSM-6) and their in vitro counterparts transformed by treatment with 60 Co gamma rays (KMST-6). Cells seeded by treatment with EDTA were cultured in a serum free medium. Proteolytic enzymes in the culture medium of cells were assayed using a synthetic substrate, N-α-(p-tosyl)-L-arginine ( 3 H) methyl ester hydrochloride. The transformed cells (KMST-6) produced a larger amount of enzymes than normal cells (KMS-6). The enzyme production in both cell lines was high in the exponential growth stage and then decreased as the cells reached confluency. The proteolytic enzymes produced by these cells were trypsin- and thrombin-like enzymes. Cell growth of KMST-6 or KMS-6 was not inhibited by the addition of protease inhibitors to the culture medium. (author)

  8. Meiosis and ISSR analysis on genetic variation of {sup 60}Co {gamma}-rays irradiated variants of cut chrysanthemum

    Energy Technology Data Exchange (ETDEWEB)

    Lili, Xing; Fadi, Chen; Hengbin, Miao [College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu (China)

    2009-08-15

    Cytology and ISSR technology were used to analyze genetic variations of {sup 60}Co {gamma}-rays induced variants of cut chrysanthemum 'Changzi', for further exploring the mechanism of irradiation and providing theoretical basis for breeding of chrysanthemum. The results showed that, meiotic abnormality ratio of lagging chromosome and chromosome bridge in variants were significantly higher than those in the control at anaphase I and II. The abnormality ratio was also raised with irradiation doses increasing. The highest ratio of two abnormal phenomena at anaphase I was 9.0%, 11.3%, and at anaphase II 15.5%, 8.6%, respectively. Polymorphic bands of 112 were amplified by 21 ISSR primers and the polymorphism rate was 71.3%, which proved that DNA changed in different degrees. These variants were divided into 5 groups by UPGMA based on Jaccard coefficient. It was observed that the clustering result related to their characters of flower shape and color variations. (authors)

  9. Tl response of KMgF3 :Lu+PTFE depending on the dose of gamma radiation of 60Co

    International Nuclear Information System (INIS)

    Lopez, A.; Gonzalez, P.R.; Furetta, C.; Azorin, J; Rivera, T.; Sepulveda, F.

    2002-01-01

    Due to the great interest in Tl dosimetry for measuring the levels of radiation doses, as environmental as in the different medical radiation applications in different laboratories of the World it is continued in the searching of new Tl materials with optimum dosimetric characteristics and low cost. In this work the obtained results when irradiating Tl dosemeters of KMgF 3 :Lu + PTFE with gamma radiation of 60 Co are presented. Such results showed that this material presents linearity of response depending on the dose between 0.016 to 100 Gy. Although it is continued with the characterization tests it is able to say that this Tl new material can be used for dosimetric aims. (Author)

  10. Effect of gamma radiation (60Co) on the growth of Aspergillus ochraceus coffee (Coffea arabica L.)

    International Nuclear Information System (INIS)

    Rodrigues, Flavio T.; Duarte, Renato C.; Fanaro, Gustavo B.; Santillo, Amanda G.; Villavicencio, Anna Lucia C.H.

    2011-01-01

    Coffee is a major product on the world market. Its acceptance depends strongly on the sensory characteristics of the beverage, that is its aroma and flavor. One of the most important aspects of coffee culture today is the cup quality as such all segments of coffee production network are concentrating efforts to improve this trait. Foods that have been contaminated with fungi may contain may produce many mycotoxins including ochratoxim A (OTA). To prevent OTA in foodstuffs, it is necessary detect the producing fungi early. Ionizing radiation is a safe, environmentally clean, energy efficient process that can be used to increase the quality and reducing the microbiological contamination of the coffee. The aim of the present study was to evaluate the effects of different gamma radiation doses (0.0, 6.0, 12.0 and 18.0 kGy) on the growth of Aspergillus ochraceus in coffee (Coffee arabica L.). The analysis were performed to determine the fungi contamination the results were expressed as the viable counts per gram of sample (CFU/g) coffee samples irradiated and unirradiated. The results shows that microbiological contamination of coffee disagrees when increase doses of irradiation. The radiation doses 6.0, 12.0 and 18.0 kGy used resulted in a elimination of the number of Aspergillus ochraceus CFU/g when compared to the nonirradiated control group. Under the present conditions, gamma radiation was found to be an alternative for the control of Aspergillus ochraceus. (author)

  11. Use of 60 Co gamma radiation to expend the shell life of packaged sliced loaves

    International Nuclear Information System (INIS)

    Nazato, R.E.S.

    1991-11-01

    The evaluation of conservation of sliced loaves (bread cut into slices). baked by five bakeries of Piracicaba, after gamma irradiation and maintained into polyethylene begs of low density, of 47,5 and 85 μm of thicknesses is shown. The sliced loaves were put into the bags and thermo-sealed by hand, like they were handled by the bakers. After this, they were irradiated with doses of 0.0: 2.0; 4.0; 6.0; 8.0 and 10.0 kGy of gamma radiation in a irradiation chamber of Cobalt-60 at a dose rate of 2,68 kGy per hour, at the room temperature (28 0 C). After irradiation the samples were maintained under at the room temperature (26 - 34 0 C), and humidity, as similar as possible to the conditions of the markets, bakeries and shops they were sold. The samples were evaluated every days and if any of them presented sign of contamination. It was threw away because it was inappropriate for human consumption. (author)

  12. The effect of 60Co-gamma radio-sterilization on Boswellia carterii essential oil composition

    Directory of Open Access Journals (Sweden)

    P. Badr

    2016-10-01

    Full Text Available Background and objectives:Gamma-irradiation is used vastly for both decontamination and sterilization of natural products; but these high-energy rays can affect heat-sensitive essential oils. Methods: The oleo-gum-resin of Boswellia carterii (Burseraceae was sterilized by γ-irradiation at dose 30 KGy. The essential oils obtained by hydrodistillation of non-irradiated Boswellia (NIB and irradiated Boswellia (IB were analyzed by GC/MS and the changes were compared. The structure of octyl acetate as the major component and marker of B. carterii was confirmed by MS/MS. Results: Twenty-five compounds comprising 99.55% of NIB oil and nineteen compounds comprising 98.61% of IB oil were identified. Major constituents which were common for both oils were octyl acetate (52.67 % in NIB, 76.51 % in IB, 1-octanol (6.37 % in NIB, 5.19 % in IB, duva-4, 8, 13-triene-1,3α diol (5.52 % in NIB, 3.94 % in IB, verticiol (13.63 % in NIB and verticillane type diterpene (5.4 % in IB they made up 78.19% and 91.04% of NIB and IB, respectively. Conclusion: Gamma irradiation was an efficient method for sterilization of Boswellia carterii oleo-gum resin, but it resulted in change in the essential oil composition particularly octyl acetate changed from 52.67% to 76.51%.

  13. Structure alteration and immunological properties of {sup 60}Co gamma rays irradiated bothropstoxin-I

    Energy Technology Data Exchange (ETDEWEB)

    Baptista, Janaina A.; Yonamine, Camila Myiagui; Caproni, Priscila; Casare, Murilo; Spencer, Patrick Jack; Nascimento, Nanci do [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)]. E-mail: janabap@gmail.com; Andrade Junior, Heitor Franco de; Vieira, Daniel Perez; Galisteo Junior, Andres Jimenez [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil); Instituto de Medicina Tropical de Sao Paulo, SP (Brazil). Lab. de Protozoologia

    2007-07-01

    About 20000 ophidic accidents are registered every year in Brazil. Serum therapy with equine antisera is the only efficient treatment. The venoms employed for immunization are fairly toxic and some venoms present low immunogenicity. Thus, the obtention of modified antigens with lower toxicity and preserved or improved immunogenicity would be useful. These toxins, when submitted to gamma radiation, in aqueous solution, present structural modifications. This occurs due to reactions with the radiolysis products of water. Some scavenger substances, such as NaNO{sub 3} and t-butanol, remove selectively the water radiolysis products. Ionizing radiation has proven to be a powerful tool to attenuate snake venoms toxicity without affecting and even increasing their immunogenic properties. However, the immune mechanisms involved in recognition, processing and presentation of irradiated antigens are yet unclear. In the present work, we investigated the immunological behavior of bothropstoxin-I (Bthx-1), before and after irradiation, in the presence of selective scavengers. Isogenic mice were immunized with either the native or the irradiated toxin, either with or without scavengers. After three immunizations, serum samples were collected and the antibody titers and isotypes were determined by Enzyme Linked Immuno Sorbent Assay. The antigenic characterization of native and irradiated bothropstoxin-I was performed by Western blot. The detection of expression of murine cytokines (IFN-{gamma} and IL-10) was analyzed by RT-PCR (Reverse Transcriptase-Polymerase Chain Reaction). According to our data, irradiation process has promoted structural modifications in the toxin, characterized by higher molecular weight forms of the protein (aggregates and oligomers). Our data also indicate that irradiated toxins, alone or in the presence of NaNO{sub 3}, an aqueous electron scavenger, were immunogenic and the antibodies elicited by them were able to recognize the native toxin. On the other

  14. Effect of the irradiation on Salmonella enteretidis var. typhimurium with gamma rays from 60Co

    International Nuclear Information System (INIS)

    Maliska, C.; Norberg, A.N.; Norberg, J.B.M.; Santos Souza, R.C. dos; Silva Tavares, P.R. da

    1988-01-01

    The use of ionizinf radiation to the destruction of microrganisms responsible for food deterioration, and productive of feeding toxinfections constitute their usefulness for actually peaceful goals of nuclear energy. The feeding toxinfections are, among us, produced in their most part by Salmonella enteritidis var. typhimurim. One hundred nineteen samples of milk containing about 150.000 bacteria per ml, by means doses ranging from 100 to 1.100 gy, two samples of surviving bacteria were again irradiated by doses up to 2.5000 Gy. The bacteria not previously irradiated were throughly killed by means of doses of 1.100 Gy. Salmonella enteritidis var. typhimurium was inactivated by means of 1.200 and 1.900 Gy doses. It was concluded that 60-Cobalt gamma radiation minimal lethal dose to Salmonella enteritidis var. typhimurium is 1.200 Gy; the re-irradiation to the survivors prompts the forthcoming of more resistant germs. (author) [pt

  15. Studies of radiation gamma effects of 60 Co on fecundity, fertility and survival of Biomphalaria straminea (Dunker, 1848)

    International Nuclear Information System (INIS)

    Albuquerque Melo, Ana M.M. de; Motta, Mauricy A. da

    1997-01-01

    The effects of gamma 60 Co radiation on fecundity, fertility and survival of Biomphalaria straminea was carried out with these mollusks kept in totality reproductive isolation and grouped, for their possible utilization in the study of radiation effect in biological system. Biomphalaria straminea species are disseminated in Brazil, specially in Northeast region. The snails were exposed to the following doses of gamma radiation: 2.5; 5.0; 7.5; 10; 15; 20; 25; 30; 35; 40; 45; 55; 60; 80; 160; 320; and 640 gy source was a Gamma Cell Co model RL (rate dose = 0,31 Gy/min). The fecundity was observed through the count of the spawing abd eggs number/snail/day; the fecundity and fertility when the radiation dose increased; it was evidenced that the grouped mollusks presented a better performance in respectivity when compared with the isolated ones. After the statistical analysis was observed that fercundity and fertility were closely related. The survival, presented higher levels of resistence in isolated mollusks than in grouped ones. The DL50/30 obtained for the isolated mollusks submitted the radiation was about 90 Gy and for the grouped ones 70 Gy. (author). 29 refs., 3 figs., 2 tabs

  16. Analysis of interface states and series resistance at MIS structure irradiated under {sup 60}Co {gamma}-rays

    Energy Technology Data Exchange (ETDEWEB)

    Tataroglu, A. [Department of Physics, Faculty of Arts and Sciences, Gazi University, 06500 Ankara (Turkey)], E-mail: ademt@gazi.edu.tr; Altindal, S. [Department of Physics, Faculty of Arts and Sciences, Gazi University, 06500 Ankara (Turkey)

    2007-10-11

    In this research, we investigated the effect of {sup 60}Co {gamma}-ray exposure on the electrical properties of Au/SnO{sub 2}/n-Si (MIS) structures using current-voltage (I-V) measurements. The fabricated devices were exposed to {gamma}-ray doses ranging from 0 to 300 kGy at a dose rate of 2.12 kGy h{sup -1} in water at room temperature. The density of interface states N{sub ss} as a function of E{sub c}-E{sub ss} is deduced from the forward bias I-V data for each dose by taking into account the bias dependence effective barrier height and series resistance of device at room temperature. Experimental results show that the {gamma}-irradiation gives rise to an increase in the zero bias barrier height {phi}{sub BO}, as the ideality factor n and N{sub ss} decrease with increasing radiation dose. In addition, the values of series resistance were determined using Cheung's method. The R{sub s} increases with increasing radiation dose. The results show that the main effect of the radiation is the generation of interface states with energy level within the forbidden band gap at the insulator/semiconductor interface.

  17. The protector role of Mn2+ in Saccharomyces cerevisiae exposed to 60Co gamma radiation

    International Nuclear Information System (INIS)

    Galvao, Izabela; Leal, Alexandre Soares; Neves, Maria Jose

    2009-01-01

    The radiation is present on all over the world and can be classified, according to the effect that produces on matter, as non-ionizing and ionizing. The ionizing radiations transfer a sufficient amount of energy to the molecule and charged atoms called ions can be formatted. The biological organisms have approximately 70% of water and when submit to the incident radiation occur the radiolysis of water. Water radiolysis produces highly reactive oxygen species (ROS) known as free radicals. The ROS can interact with all important biomolecules like lipid, protein and DNA. This interaction can cause the loss of functions of theses molecules and thus lead to cell death. The manganese is a heavy metal that at low concentrations is essential for the biological system, since it is cofactor for action of several enzymes. At high concentrations the manganese is toxic to the organisms. Recently was observed that manganese can have a protector role against oxidative stress. In this work, it was studied the anti-oxidant role of manganese in cells submitted to gamma irradiation. It was performed the determination of two oxidative stress indicators: lipid peroxidation and determination of thiol group, the results presented suggest that the manganese had protective role against lipid damage and in this way, has an antioxidant role. (author)

  18. Structure alteration and immunological properties of 60Co gamma rays irradiated bothropstoxin-I

    International Nuclear Information System (INIS)

    Baptista, Janaina A.; Yonamine, Camila Myiagui; Caproni, Priscila; Casare, Murilo; Spencer, Patrick Jack; Nascimento, Nanci do; Andrade Junior, Heitor Franco de; Vieira, Daniel Perez; Galisteo Junior, Andres Jimenez; Instituto de Medicina Tropical de Sao Paulo, SP

    2007-01-01

    About 20000 ophidic accidents are registered every year in Brazil. Serum therapy with equine antisera is the only efficient treatment. The venoms employed for immunization are fairly toxic and some venoms present low immunogenicity. Thus, the obtention of modified antigens with lower toxicity and preserved or improved immunogenicity would be useful. These toxins, when submitted to gamma radiation, in aqueous solution, present structural modifications. This occurs due to reactions with the radiolysis products of water. Some scavenger substances, such as NaNO 3 and t-butanol, remove selectively the water radiolysis products. Ionizing radiation has proven to be a powerful tool to attenuate snake venoms toxicity without affecting and even increasing their immunogenic properties. However, the immune mechanisms involved in recognition, processing and presentation of irradiated antigens are yet unclear. In the present work, we investigated the immunological behavior of bothropstoxin-I (Bthx-1), before and after irradiation, in the presence of selective scavengers. Isogenic mice were immunized with either the native or the irradiated toxin, either with or without scavengers. After three immunizations, serum samples were collected and the antibody titers and isotypes were determined by Enzyme Linked Immuno Sorbent Assay. The antigenic characterization of native and irradiated bothropstoxin-I was performed by Western blot. The detection of expression of murine cytokines (IFN-γ and IL-10) was analyzed by RT-PCR (Reverse Transcriptase-Polymerase Chain Reaction). According to our data, irradiation process has promoted structural modifications in the toxin, characterized by higher molecular weight forms of the protein (aggregates and oligomers). Our data also indicate that irradiated toxins, alone or in the presence of NaNO 3 , an aqueous electron scavenger, were immunogenic and the antibodies elicited by them were able to recognize the native toxin. On the other hand, when the

  19. Displacement correction factor versus effective point of measurement in depth dose curve measurements at {sup 60}Co gamma rays

    Energy Technology Data Exchange (ETDEWEB)

    Bruna, A [Universidad Nacional, Cordoba (Argentina). Facultad de Matematica, Astronomia y Fisica; Velez, G R [Hospital San Roque, Cordoba (Argentina). Dept. de Radioterapia; Brunetto, M [Centro Medico Rivado Dean Funes, Cordoba (Argentina)

    1996-08-01

    The discrepancies in data sets of values of the Displacement Factor p{sub d} recommended by different codes of practices for calibration purpose still demand further investigation to clarify this point. In this paper, we propose an experimental method to determine the displacement factor for cylindrical ionization chambers (thimble chambers) in photon beams. Measurements of p{sub d} for several depths were performed for {sup 60}Co gamma rays. From these results we calculated the shift of the effective point of measurement (z-z{sub eff}) for different depths. The results obtained in this work shown: (a) there is no significant change in p{sub d} from 2 cm to 17 cm of depth in water; (b) the value of p{sub d} for a ion-chamber Farmer type (inner radius r = 3.15 cm) is p{sub d} 0.988; (c) the shift of the effective point of measurement has a smooth variation with depth; (d) the value of (z-z{sub eff}) at the recommended calibration depth for {sup 60}Co beams (5 cm) is 0.6r (with r: inner radius of the chamber). The result (b) confirms the value of p{sub d} suggested by the SEFM and NACP protocols and differs with that of the AAPM. The value obtained for (z - z{sub eff}) (d) is very closed to that recommended by the IAEA TRS-277. Finally, the results (a) and (c) suggest that it should be preferable to use the displacement factor instead of effective point of measurement to perform measurements of depth dose curves, since the use of z{sub eff} should take into account its dependence on depth. (author). 7 refs, 4 figs.

  20. Aspects of the immune response against proteic antigens submitted to the effects of 60Co gamma radiation

    International Nuclear Information System (INIS)

    Alves, Janaina Baptista.

    2004-01-01

    Considering the effects of gamma radiation on proteins and the capacity of the immune system to recognize modified macromolecules, we decided to evaluate some immunological aspects of B10.PL mice exposed to native or irradiated ovalbumin and bothrops toxin-1 (BthTx-1). In order to evaluate possible structural modifications of the molecules after being irradiated ( 60 Co gamma rays), bothrops toxin-1 was analysed by electrophoresis, while ovalbumin was submitted to analytical size exclusion chromatography. The toxin was also analysed by ESI-mass spectrometry. Our results indicate that radiation promoted modifications on both the molecules. Aiming to compare the toxicity of the native and irradiated forms of the toxin, an in vitro cytotoxicity assay, using CHO cells, was performed. According to our results, the modified toxin was 5 folds less toxic than its native counterpart. Sera of animals immunized with the native and irradiated proteins were analyzed in order to evaluate levels of IgG, as well as to quantify specific isotopes. While the native proteins induced a predominant Th2 response, the irradiated molecules apparently promoted a switch towards a Th1 pattern. We also performed a cell proliferation assay with splenocytes from mice immunized with either the native or the irradiated proteins, cultured in the presence of the antigens. Our results indicate that both the forms of the proteins induced a similar proliferative response. These data indicate a potential use of detoxified proteins as antigens for immunization. (author)

  1. Evaluation of fruit productivity and quality in Hass avocado submitted to {sup 60}Co gamma radiation; Evaluacion de productividad y calidad de fruta en aguacate Hass sometido a radiacion gamma de {sup 60}Co

    Energy Technology Data Exchange (ETDEWEB)

    Cruz T, E. De la; Ibanez P, J.; Mijares O, P.; Garcia A, J.M. [Instituto nacional de Investigaciones Nucleares, Departamento de Biologia, A.P. 18-1027, 11801 Mexico D.F. (Mexico)

    1999-07-01

    Evaluation of productivity, postharvest behavior and fruit quality was performed on four years Hass avocado trees irradiated with {sup 60} Co gamma rays in doses of 5, 10, 15, 20 and 25 Gy, established in the ''La Labor'' Experimental Center of the Centro de Investigaciones Cientificas y Tecnologias del Aguacate en el Estado de Mexico (CICTAMEX) at Temascaltepec Mexico. Productivity had a significant increase in the dose of 15 Gy being the average number of fruits nearly 400 % more than the control at fruit setting, being such difference reduced at fruit harvesting to 300 %. In regard to postharvest performance, the respiration index (mg CO{sub 2}/kg/hr) did not show significant differences among treatments. Also others variables such as physiological weight losses, texture, maturity pattern, and sensorial tests (color, flavor, aroma, texture) were not different in regard to the control. This mean that radiation has altered productivity but not the quality and postharvest behavior of fruits. (Author)

  2. The optimal dosage of 60 co gamma irradiation for obtaining salt gland mutants of exo-recretohalophyte limonium bicolor (bunge) o. kuntze

    International Nuclear Information System (INIS)

    Yuan, F.; Chen, M.; Yang, J.; Wang, B.

    2015-01-01

    Limonium bicolor (Bunge) O. Kuntze is a typical exo-recretohalophyte with multi-cellular salt glands. It is often used to improve saline-alkali soil. Seeds of L. bicolor were treated with different doses of 60 Co gamma irradiation to determine the LD50 for 60 Co gamma irradiation; the goal was to produce a relatively high number of mutants in salt gland development and salt secretion with a relatively low level of mortality. 60 Co gamma irradiation did not greatly affect germination, but an increase in gamma dose prevented the development of true leaves and reduced the percentage of seedlings that emerged from soil. The LD50 for 60 Co gamma irradiation was 120 Gy. Two mutants (few and many) were obtained under the LD50 using the screening methods - differential interference contrast microscope and leaf discs excretion model. Compared with the wild type, few and many had mutation in salt gland development, and many showed lower salt secretion rate per single salt gland than WT. These mutants would provide insight into the molecular mechanisms of salt gland development and salt secretion and into the development of salt-tolerant crop plants. (author)

  3. Enhancement of epoxide hydrolase production by 60 Co gamma and UV irradiation mutagenesis of Aspergillus niger ZJB-09103.

    Science.gov (United States)

    Jin, Huo-Xi; OuYang, Xiao-Kun; Hu, Zhong-Ce

    2017-05-01

    An effective epoxide hydrolase (EH) production strain was mutagenized using 60 Co gamma and UV irradiation. Among positive mutant strains, the EH activity of C2-44 reached 33.7 U/g, which was 267% as much as that of the original Aspergillus niger ZJB-09103. Compared with the wild type, there were significant changes in morphology for C2-44, including the color of mycelia on the slants and the shape of conidial head. In addition, glucose and soybean cake were the optimal carbon and nitrogen source in terms of EH activity for the mutant C2-44 instead of soluble starch and peptone for the wild-type strain. The reaction time required to reach 99% enantiomeric excesses of (S)-epichlorohydrin from racemic substrate was shortened significantly by the mutant C2-44. This phenomenon was probably explained by the higher V max for hydrolysis of racemic epichlorohydrin by C2-44 compared with Aspergillus niger ZJB-09103. © 2016 International Union of Biochemistry and Molecular Biology, Inc.

  4. The effect of build-up cap materials on the response of an ionization chamber to 60Co gamma rays

    International Nuclear Information System (INIS)

    Rocha, M.P.O.; Almeida, C.E. de

    1993-01-01

    Knowledge of the effect of wall and build-up cap materials on ionization chamber response is necessary to determine absorbed dose in a medium using a calibration factor based on exposure or kerma in air. Attenuation and scattering effects of 60 Co gamma rays in the ionization chamber wall and build-up cap, as well as their non-equivalence to air, were studied with an OFS ionization chamber (Delrin wall) and a set of build-up caps specially built for this purpose. Results for a specific material were plotted as functions of wall and cap total thickness, extrapolated to zero wall thickness, then corrected for mean centre of electron production in the wall (= 0.136 g cm -2 ). Correction factors for a specific thickness were analysed in relation to cap material, and to relative responses compared with values calculated by using AAPM, SEFM and IAEA formalisms for cap effects. A Monte Carlo calculation was performed to compare the experimental and theoretical values. Calculations showed an agreement within 0.1% with experimental values and a wall effect of approximately 1.6%. (Author)

  5. Study of gamma radiation from 60Co effects on Apis mellifera venom: biochemical, pharmacological and immunological aspects

    International Nuclear Information System (INIS)

    Costa, Helena

    2001-01-01

    Africanized honeybees are very common insects in Brazil and frequently cause accidents followed by important immunological reactions and even deaths. Their venoms are composed of a complex mixture of substances of general biological actions. Ionizing radiation is able to modify molecular structures affecting the biological properties of proteins. It decreases toxic and enzymatic activities and so, it appears promising as a venom detoxification tool. The main objective of this work was to study the effects of gamma radiation on bee venom, regarding biochemical, pharmacological and immunological aspects. Africanized Apis mellifera whole venom (2 mg/ml) in 0.15 M NaCl solution was irradiated with 2 kGy in a 60 Co source. Native and irradiated bee venoms were submitted to high performance size exclusion chromatography (Tosohaas G2000SW column), high performance reversed phase chromatography in a C-18 column under water/acetonitrile gradient, SDS-PAGE. For both venoms studies have been carried out in UV absorption spectrum, protein concentration, hemolytic activity, and PLA 2 activity analysis, lethality assay (LD 50 ). Biodistribution studies was carried out after labelling native and irradiated bee venom with 99m Tc. The results showed that gamma radiation did not change the protein concentration nor its immunogenicity, although it could be observed that irradiated bee venom UV spectrum and SDS-PAGE profile presented differences when compared to native bee venom. This suggests that some structural alterations in bee venom components could have occurred after irradiation. HPLC-RP profiles showed that gamma radiation could have caused conformational changes, such as unfolding of molecule chains, changing their hydrophobic groups exposuring. The hemolytic and the PLA 2 activities of irradiated bee venom were smaller than the native ones. The gamma radiation diminished the toxicity of bee venom, but did not abolish its bioactivity, like hemolysis. Biodistribution studies

  6. Contribution to the study of the behaviour of silicon planar transistors exposed to the {sup 60}Co {gamma} rays; Contribution a l'etude du comportement des transistors silicium a structure plane soumis aux rayons {gamma} du {sup 60}Co

    Energy Technology Data Exchange (ETDEWEB)

    Le Ber, J [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1967-05-15

    This report gives an account of studies carried out on bipolar silicon planar transistors irradiated by {sup 60}Co {gamma} rays. The author describes the interactions on the matter of the different types of particles and he gives a brief bibliographical recall of foreign studies. The technological structure of the planar transistors is then described in order to help the understanding of the phenomena, general comments are made about the choice of measured parameters and on the statistical interpretation of results. An automatic instrument for the measurement of the gain is described and the reproducibility of the results is stated The complexity of the problem and the difficulty to predict the behaviour of the semiconductors components are clearly shown. It is stated that the observed dispersions depend on: - the electrical bias during irradiation - the injection level in the emitter-base junction during the measurement - the manufacturer for a given type - the instantaneous dose rate - the geometry used The problem is then examined from the reliability point of view and methods are given to evaluate the reliability for a given dose - 'Worst case' method - moment method - Monte Carlo method. (author) [French] Ce rapport rend compte du travail effectue sur les transistors bipolaires au silicium irradies au rayons {gamma} du cobalt 60. On passe en revue les mecanismes d'interaction des differents rayonnements avec la matiere et on fait un bref rappel bibliographique des etudes effectuees a l'etranger. On decrit ensuite la structure technologique du transistor pour aider a la comprehension des phenomenes, puis on donne des generalites sur le choix des parametres mesures et l'interpretation statistique des resultats. On decrit l'ensemble du systeme de mesure de gain et on s'attache a montrer la reproductibilite des mesures. Les resultats experimentaux mentionnes etablissent clairement la complexite du probleme et la difficulte qu'il y a de faire des previsions. On

  7. Some Results Of Gamma-60Co Irradiation Application To Breed New Soybean Varieties At Institute Of Agricultural Genetics, 2001 - 2007

    International Nuclear Information System (INIS)

    Mai Quang Vinh; Pham Bao Chung; Ngo Phuong Thinh; Ngo Khanh Phuong; Le Van Nam

    2008-01-01

    To overcome the disadvantage characters and continue create new genetical materials in new Soybean variety selection to get high yield, resistance to with disadvantage conditions, good quality. The Institute of Agricultural Genetics successfully combinated the good particularities of mutant varieties DT84/DT83 and received DT2001 variety, and from mutant DT90/DT84 received new variety DT96. These varieties quite resists with rare good drought-tolerant, high yield from 2000 to 3500 kg/ha, appropriate with all of 3 crops: spring, summer and winter and have wide adaptation. DT96, DT2001 varieties were adopted by MARD Scientific Council as New National Varieties. cooperated with Dalat Institute of Nuclear research treated the Gamma - 60 Co Irradiation on Soybean and Vegetable Soybean. Irradiated dry seed of 3 varieties DT96, DT2001, DT158 with dose levels of 150, 200, 250 Gy in order to shorten the rise time and strengthen the drought resistance. At M3 generation had selected 3 lines D.96/26, D.01/58, D.58/08, D.58/224, which have rise time from 3 to 5 day shorter and yield higher than control (D.96/150, D.01/245). Irradiated the 2 vegetable Soybean DT02 (to ameliorate its thick shell, improve sweetness and resist with collapse) and DT06 (to ameliorate its heat resistance) with dose levels of 100, 140, 180, 220 Gy were received the generations and now continue being researching the M3 generation in 2007 summer crop. (author)

  8. Resveratrol effects on life span and fertility of caenorhabditis elegans subject to 60Co gamma ray irradiation

    International Nuclear Information System (INIS)

    Ye Kai; Ji Chenbo; Guo Xirong; Gu Guixiong

    2011-01-01

    Caennorhabditis elegans was used as experimental model to investigate radiation effect of resveratrol on caenorhabditis elegans irradiated by 60 Co γ ray. Treatment with resveratrol can increase average life span and spawning rate, improve the survival rate of eggs, and protect their mitochondrion function of caenorhabditis elegans exposure to 60 Co γ ray. The results indicate that resveratrol has radiation protection effects, which might be related to its action on ROS decrease and mitochondrial defend. (authors)

  9. Investigations on Pasteurization of Cold Marinades by {sup 60}Co Gamma Rays; Recherches sur la Pasteurisation a Froid De Marinades par les Rayons Gamma du {sup 60}Co; Issledovaniya pasterizatsii kholodnykh marinadov gamma-luchami; Investigaciones Sobre la Pasteurizacion de Escabeches Frios por Irradiacion Gamma con {sup 60}Co

    Energy Technology Data Exchange (ETDEWEB)

    Schoenborn, W.; Kinkel, R J.; Hafferl, W. [Battelle Institut e.V., Frankfurt/Main, Federal Republic of Germany (Germany)

    1966-11-15

    Marinated herring fillets are a fish product matured under the action of acetic acid and salt and marketed in a spiced brine containing about 3% salt and acetic acid (pH 4). Since the usual addition of the preservative hexamethylene-tetramine has been restricted to a limited period of time by the new German food law, investigations were made on pasteurization by means of ionizing rays, as a new method of preservation without the addition of other preservatives. Decay of this food product was caused by heterofermentative lactic-acid bacteria, which decarboxy- lated the amino acids set free from the protein; the decay was a result of carbon dioxide bombardment. The marinades were packed into flat tins holding about 125 g, and exposed to {sup 60}Co gamma rays from an under-water source, while being cooled. The shelf life of the tinned marinades was extended by the irradiation. The period up to bombardment at 15 Degree-Sign C was three times as long as usual in cases of irradiation with 155 krad. The critical number of 1 million germs per ml at 15 Degree-Sign C was also reached after a period three times as long as usual upon irradiation with 160 krad. The LD{sub 99} of three isolated strains of lactic-acid bacteria irradiated in a salt-peptone solution was 113, 165 and 144 krad, respectively. Since at 15 Degree-Sign C the growth of the germs is retarded, while at 20 Degree-Sign C it is practically unrestrained, the period of durability at a storage temperature of 18 Degree-Sign C could only be doubled by irradiation with 280 krad. The organoleptic tests were based on preference analyses by means of the nine-step Hedonic scale, and on difference tests (triangle test) and were performed by a paneli At 70 krad the taste is still acceptable at 140 krad there is a distinct taste of rays and at 280 krad the taste is disagreeable. A distinct improvement in taste is achieved by irradiation at low temperatures (-30 Degree-Sign C) and by the addition of ascorbic acid, sorbitol

  10. Resposta de sementes de amendoim a diferentes doses de radiação gama (60Co Response of peanut seeds to different levels of gamma radiation (60Co

    Directory of Open Access Journals (Sweden)

    Tatiana da S. Santos

    2010-10-01

    Full Text Available A radiação gama, proveniente da fonte de 60Co, é bastante utilizada para esterilização, visando a prevenção da decomposição e a toxidez de origem microbiana em diversos produtos. O grau de radiossensibilidade de um embrião vegetal depende da espécie, do estágio de seu desenvolvimento durante a radiação, da dose empregada e do critério usado para medir o efeito biológico, sendo comumente utilizado o teste de germinação. Objetivou-se, com este trabalho, estudar a sensibilidade do amendoim a radiação gama e seus efeitos na germinação, no vigor e na micoflora das sementes da cultivar BRS Havana, irradiadas com uma fonte de 60Co, tipo gammacell, com taxa de dosagem de 12,5 kGy h-1. As doses testadas em kGy foram as seguintes: 0; 0,5; 1,0; 1,5; 2,0; 2,5; 3,0; 6,0; 9,0; 12,0; 15,0; 18,0; 21,0 e 24,0. Com os resultados obtidos, concluiu-se que as doses acima de 3,0 kGy prejudicaram a viabilidade das sementes e as doses acima de 12 kGy comprometeram totalmente o vigor e a germinação das sementes de amendoim. A radiação a partir da dose 2,0 kGy eliminou os fungos Aspergillus flavus e Aspergillus niger e, a partir da dose 3,0 kGy, eliminou o Aspergillus glaucus. O Penicilium spp. permaneceu em mais de 30% das sementes em todos os tratamentos com radiação, não sendo eliminado até a dose de 24 kGy.The gamma radiation from 60Co source is widely used for sterilization aiming at preventing decomposition and toxicity from microbes in several products. The degree of radiosensitvity of a plant embryo depends on the species, the development stage during radiation, doses used and the criteria used to measure the biological effect, the germination test, being commonly used.This work aimed to study the peanut sensitivity to gamma radiation and its effects in the germination, in the vigour and seeds microflora of cultivar BRS Havana, irradiated with 60Co source, type gammacell with rate of dosage of 12.5 kGy h-1. The tested doses were the

  11. Biochemical and pharmacological studies of native and irradiated crotamine with gamma radiation of {sup 60}Co; Estudo bioquimico e farmacologico das crotaminas nativa e irradiada com radiacao gama de {sup 60}Co

    Energy Technology Data Exchange (ETDEWEB)

    Mitake, Malvina Boni

    2000-07-01

    Ionizing radiation can change the molecular structure and affect the biological properties of biomolecules. This has been employed to attenuate animal toxins. Crotamine is a strongly basic polypeptide from the South American rattlesnake venom, composed of 42 amino acid residues. It induces skeletal muscle spasms leading to a spastic paralysis of hind limbs in mice. The objective of this thesis was carry out biochemical and pharmacological studies of native and irradiated crotamine with {sup 60} Co. Crotamine was purified from Crotalus durissus terrificus venom by Sephadex G-100 gel filtration followed by ion exchange chromatography, using a Fast Performance Liquid Chromatography (FPLC) system. It was irradiated at 2 mg/ml in 0.15 M Na Cl with 2.0 kGy gamma radiation emitted by a {sup 60} Co source. The native and irradiated crotamine were evaluated by biochemical characterization, toxic activity (LD{sub 50} and biodistribution. The native and irradiated crotamine were labelled with 29.6 MBq of {sup 125} I using chloramine T method, and separated in a Sephadex G-50 column. Male Swiss mice (35{+-} 5 g), were injected i.p. with o.1 mL (2.4 x 10{sup 6} cpm/mouse) of {sup 125} I native crotamine or with 0.4 mL (1.3 x 10{sup 6} cpm/mouse) of {sup 125} I irradiated crotamine. At 0.08; 0.25; 0.5; 1; 2; 4; 8; 12 and 24 hours the animal were killed by ether inhalation. Blood, spleen, liver, kidneys, brain, lungs, heart, and skeletal muscle were collected in order to determine radioactivity content. The results showed that gamma radiation did not change the protein concentration, the electroforetic profile or the primary structure of the protein, although differences were shown by spectroscopic techniques. The gamma radiation diminished the toxicity of crotamine, but it did not abolish bioactivity. Biodistribution studies showed that native and irradiated crotamine have hepatic metabolism and renal elimination. The native and irradiated crotamine have affinity by skeletal

  12. Gamma radiation effects of {sup 60} Co on Bombyx mori (Lep., Bombycidae) modifying the silk fiber production; Influencia da radiacao gama ({sup 60} Co) na producao de fios de seda em Bombyx mori(Lep.,Bombycidae)

    Energy Technology Data Exchange (ETDEWEB)

    Carneiro Junior, Francisco [Universidade Metodista de Piracicaba (UNIMEP), SP (Brazil); Bendassolli, Jose A. [Centro de Energia Nuclear na Agricultura (CENA), Piracicaba, SP (Brazil)

    1997-12-01

    The present work aimed to verify the biological effects of the application of different doses of gamma radiation during the fifth instar of the silkworm catepillar. Sevently eight silkworm caterpillars (Bombyx mori) were irradiated with {gamma}{sup 60} Co radiation at the initial period of the fifth instar. The caterpillars were divided and classified in six batches of thirteen individuals each. Treatments 1 through 5 received 20, 40, 60, 80 and 100 Gy, respectively, and the control, also consisted of thirteen caterpillars, was not irradiated. The results showed a general increase in the silk fiber content in the irradiated batches compared to the control. The weight of the silk cocoons was higher with increasing doses of irradiation, from 20 to 80 Gy, respectively, followed by a decrease in weight in the treatment irradiated with 100 Gy. the results obtained in this experiment enable the conclusion that the radiation applied to the caterpillars significantly influenced the production of silk fiber in this species. (author). 4 refs., 2 figs., 3 tabs.

  13. Effect of gamma radiation of 60Co in the variability of chinese bean [Vigna unguiculata (l.) Walpers] en R4M4

    International Nuclear Information System (INIS)

    Salmeron E, J.; Bueno J, J.E.; Valencia E, F.; Cervantes S, T.; Cruz T, E. De la

    2007-01-01

    Selection of plants of Chinese bean coming from seed irradiated with gammas of 60 Co in the generation R 4 M 4 (fourth recurrent irradiation, fourth segregate generation) were carried out, taking as selection approaches the plant architecture, the numbers of sheaths for plant, sheath longitude, position of the sheaths, grain size and resistance to plagues and illnesses. 17 lines were selected for grain and 3 lines with fodder characteristics of black grain color were also obtained. (Author)

  14. Comparative studies on the effect of negative pions, 60Co gamma irradiation and hyperthermia on an in vitro 'tumour model'

    International Nuclear Information System (INIS)

    Luecke-Huhle, C.; Dertinger, H.; Schlag, H.; Weibezahn, K.F.

    1977-01-01

    Multicellular spheriods of Chinese hamster cells (V79) are much more resistant than monolayer cultures of the same cell line; this is observed after 60 Co γ-irradiation, after hyperthermic treatment, after combined treatment of heat with 60 Co γ-radiation and after exposure to negative pi-mesons. Intercellular communication within the three-dimensional cell clones seems to be responsible for an enhanced repair capacity for sub-lethal as well as for potentially lethal damage. While the response of spheroid cells to 60 Co γ-irradiation is almost independent of the state of growth, there is a clear difference in survival whether exponentially growing outer cells or inner plateau phase cells are exposed to heat: moderate heat treatment (4h at 42 0 C) leads to a greater killing efficiency of exponentially growing cells while the inner non-cycling cells exhibit a recruitment of resting cells into the proliferative compartment as demonstrated by means of flow-microfluorometry. Hyperthermia in combination with 60 Co γ-irradiation was shown to eliminate hypoxic cells and to yield a thermal enhancement ratio comparable to the RBE value obtained after pion irradiation at maximum LET. (author)

  15. 60Co gamma irradiation effects on the the capacitance and conductance characteristics of Au/PMI/n-Si Schottky diodes

    Science.gov (United States)

    Tuğluoğlu, N.; Karadeniz, S.; Yüksel, Ö. F.; Şafak, H.; Kuş, M.

    2015-08-01

    In this work, the perylene-monoimide/n-Si (100) Schottky structures have been fabricated by spin coating process. We have studied the capacitance-voltage ( C- V) and conductance-voltage ( G- V) characteristics of the Au/perylene-monoimide/n-Si diodes at 500 kHz before and after 60Co γ-ray irradiation. The effects of 60Co γ -ray irradiation on the electrical characteristics of a perylene-monoimide/n-Si Schottky diode have been investigated. A decrease both in the capacitance and conductance has been observed after 60Co γ -ray irradiation. This has been attributed to a decrease in the net ionized dopant concentration that occurred as a result of 60Co γ-ray irradiation. Some contact parameters such as barrier height (Φ B ) interface state density ( N ss ) and series resistance ( R s ) have been calculated from the C- V and G- V characteristics of the diode before and after irradiation. It has been observed that the Φ B and N ss values are decreased after the applied radiation, while the R s value is increased.

  16. Ameliorative effect of salicylic acid and theophylline on photosynthetic pigment content in gamma irradiated french bean varieties, using "6"0Co as a source

    International Nuclear Information System (INIS)

    Shukla, Pradeep K.; Vishwakarma, Kapil Kumar; Shukla, Saumya; Sharma, Richa; Ramteke, P.W.; Misra, Pragati

    2017-01-01

    Irradiation of seeds may cause genetic variability that enable plant breeders to select new genotypes with improved qualitative and quantitative characteristics. An experiment was conducted to study the protective role of salicylic acid and theophylline on photosynthetic pigments of gamma exposed french bean. Seeds of four French bean were treated by different doses of gamma radiation using "6"0Co as source. The results showed that the application of salicylic acid and theophylline significantly increased chlorophyll a content, chlorophyll b content, total chlorophyll content and carotenoid content. Salicylic acid was more effective than theophylline in overcoming the radiation effects and therefore, showed more protection to the photosynthetic pigments. (author)

  17. Influence of source configuration on spectral composition of gamma-ray beams from 60Co teletherapy units

    International Nuclear Information System (INIS)

    Ehrlich, M.; Soares, C.G.; Jackson, B.; Lanoue, P.

    1978-01-01

    Measurements were made of the photon spectra of simulated 60 Co teletherapy beams. Various source configurations and source environments of practical interest were employed for this purpose. The sources consisted of activated cobalt pellets packed into steel capsules. Several combinations of capsule diameters and heights of pellet layers were used. The spacer materials filling the remaining capsule volume were chosen to be of high, intermediate, or low atomic number. The capsules could be inserted, one at a time, into a compartment of either tungsten or brass, simulating the central section of popular commercial 60 Co teletherapy units. There also was some choice in the atomic number of the structural elements holding the capsules in place in the compartment. The largest contribution of scattered photons was found to occur when the materials close to the source pellets had atomic numbers in the vicinity of 30. (author)

  18. Study of gamma radiation from {sup 60}Co effects on Apis mellifera venom: biochemical, pharmacological and immunological aspects; Estudo dos efeitos da radiacao gama de {sup 60}Co na peconha de Apis mellifera: aspectos bioquimicos, farmacologicos e imunologicos

    Energy Technology Data Exchange (ETDEWEB)

    Costa, Helena

    2001-07-01

    Africanized honeybees are very common insects in Brazil and frequently cause accidents followed by important immunological reactions and even deaths. Their venoms are composed of a complex mixture of substances of general biological actions. Ionizing radiation is able to modify molecular structures affecting the biological properties of proteins. It decreases toxic and enzymatic activities and so, it appears promising as a venom detoxification tool. The main objective of this work was to study the effects of gamma radiation on bee venom, regarding biochemical, pharmacological and immunological aspects. Africanized Apis mellifera whole venom (2 mg/ml) in 0.15 M NaCl solution was irradiated with 2 kGy in a {sup 60}Co source. Native and irradiated bee venoms were submitted to high performance size exclusion chromatography (Tosohaas G2000SW column), high performance reversed phase chromatography in a C-18 column under water/acetonitrile gradient, SDS-PAGE. For both venoms studies have been carried out in UV absorption spectrum, protein concentration, hemolytic activity, and PLA{sub 2} activity analysis, lethality assay (LD{sub 50}). Biodistribution studies was carried out after labelling native and irradiated bee venom with {sup 99m}Tc. The results showed that gamma radiation did not change the protein concentration nor its immunogenicity, although it could be observed that irradiated bee venom UV spectrum and SDS-PAGE profile presented differences when compared to native bee venom. This suggests that some structural alterations in bee venom components could have occurred after irradiation. HPLC-RP profiles showed that gamma radiation could have caused conformational changes, such as unfolding of molecule chains, changing their hydrophobic groups exposuring. The hemolytic and the PLA{sub 2} activities of irradiated bee venom were smaller than the native ones. The gamma radiation diminished the toxicity of bee venom, but did not abolish its bioactivity, like hemolysis

  19. (60)Co in cast steel matrix: A European interlaboratory comparison for the characterisation of new activity standards for calibration of gamma-ray spectrometers in metallurgy.

    Science.gov (United States)

    Tzika, Faidra; Burda, Oleksiy; Hult, Mikael; Arnold, Dirk; Marroyo, Belén Caro; Dryák, Pavel; Fazio, Aldo; Ferreux, Laurent; García-Toraño, Eduardo; Javornik, Andrej; Klemola, Seppo; Luca, Aurelian; Moser, Hannah; Nečemer, Marijan; Peyrés, Virginia; Reis, Mario; Silva, Lidia; Šolc, Jaroslav; Svec, Anton; Tyminski, Zbigniew; Vodenik, Branko; Wätjen, Uwe

    2016-08-01

    Two series of activity standards of (60)Co in cast steel matrix, developed for the calibration of gamma-ray spectrometry systems in the metallurgical sector, were characterised using a European interlaboratory comparison among twelve National Metrology Institutes and one international organisation. The first standard, consisting of 14 disc shaped samples, was cast from steel contaminated during production ("originally"), and the second, consisting of 15 similar discs, from artificially-contaminated ("spiked") steel. The reference activity concentrations of (60)Co in the cast steel standards were (1.077±0.019) Bqg(-1) on 1 January 2013 12h00 UT and (1.483±0.022) Bqg(-1) on 1 June 2013 12h00 UT, respectively. Copyright © 2016 The Authors. Published by Elsevier Ltd.. All rights reserved.

  20. DNA damage and γH2AX expression in EJ cells induced by 60Co gamma-rays

    International Nuclear Information System (INIS)

    Pan Yan; Tian Mei; Liu Jianxiang; Ruan Jianlei; Su Xu

    2009-01-01

    Objective: To investigate 60 Co γ-rays induced DNA damage of human bladder cancer cell line EJ cells and the relationship between different doses of 60 Co γ-rays, γH2AX foci number and γH2AX expression level. Methods: EJ cells were exposed to different doses of 60 Co γ-rays and the oliver tail moment (TM) of EJ cells were analyzed with single cell gel electrophoresis (SCGE) . Immunofluorescent microscopy was used to analysis γH2AX foci formation in EJ cells after exposure to different doses of γ-ray irradiation and time-course after exposure to 2 Gy γ-ray irradiation. FACSAria was used to detect the changes of γH2AX protein expression in EJ cells. Results: The TMs of EJ cells were increased with the irradiation dose. The TM of 0 Gy group and 4 Gy group was 0.24 and 5.26, respectively. Immunofluorescent analysis demonstrated that γH2AX foci could be induced by γ-ray irradiation in dose-dependent and time-dependent manners. The foci number and size in nuclei of EJ cells was significantly increased after exposed to different doses of γ-ray irradiation and the foci remained detectable at 24 h after exposed to irradiation. The dose range in which foci could be clearly detected was from 0.1 to 4 Gy. FACSDiva showed that γH2AX protein expression was increased after exposure to different doses of γ-ray irradiation. γH2AX protein expression level of 0.1 Gy group and 4 Gy group was 7.4% and 29.2% , respectively. Conclusions: γH2AX foci could be the most sensitive indicator for DNA damage and repair in mammalian cells, and it might be a new biomarker for radiological emergency. (authors)

  1. Evaluation of photosynthetic pigments and protein content in some gamma treated wheat varieties using 60Co as a source

    International Nuclear Information System (INIS)

    Mishra, Sandeep; Shukla, Pradeep K.; Ramteke, P.W.; Misra, Pragati; Shukla, Narayani; Gautam, Sanghdeep; Gayatri

    2014-01-01

    Gamma rays are often used on plants in developing varieties that are agriculturally and economically important and have high productivity. The results showed that variety PBW-154 was relatively tolerant to gamma radiation among all the verities, whereas, HD-2733 and LOK-1 were sensitive to gamma radiation varieties. The biochemical parameters in all wheat varieties, chlorophyll content and protein content, showed a significant decrease with the increase in treatment of gamma radiation stress

  2. Study about the sensibility in vitro of different strains of Vibrio cholera 01 exposed to 60 Co gamma radiation

    International Nuclear Information System (INIS)

    Moraes, Ivany Rodrigues de

    1998-01-01

    The presence of some microorganisms in food, or the metabolites originated during their own multiplication may bring several diseases to humans: intoxications and food borne infections. Among the agents that may cause those diseases, we find Vibrio cholerae 01. In this experiment, the studies are focused on the radiosensibility in vitro of four strains of V. cholerae 01, exposed to different doses of ionizing radiation of 60 Co. The results are compared with other data related to bacterial food borne diseases, including water. (author)

  3. Study of the ionization of alkane-electron scavenger reactant mixtures irradiated by 60Co gamma rays

    International Nuclear Information System (INIS)

    Bonnet, Jacques.

    1977-01-01

    This study deals with ionization of alkane-electron scavenger reactant mixtures, irradiated by 60 Co γ-rays. It is shown that the extrapolated free-ion yields (extrapolated yield method) decrease with the reactant concentration. On the basis of ONSAGER model and theoretical treatment of MOZUMDER, the cross sections of epithermal electron attachment in hexane, cyclohexane, 2,2-dimethylbutane, cyclopentane, 2,2,4-trimethylpentane for CCl 4 , C 7 F 14 , C 6 H 5 Br, C 6 H 5 Cl, C 6 F 14 , (C 6 H 5 ) 2 are determined. A comparison between gas-phase and liquid-phase cross sections is established [fr

  4. Dosimetry of a gamma beam - 650 60Co irradiator. 3. Mapping of the isodose curves in the internal cavity

    International Nuclear Information System (INIS)

    Escobedo, J.F.; Nascimento Filho, V.F. do; Ferraz, E.S.B.

    1981-01-01

    The Gammabeam-650 60 Co irradiator, containing 29,080 curies (April 1 sup(st), 1974), made by Atomic Energy of Canada Limited and in operation at CENA - Piracicaba, Sao Paulo, Brazil, basically has a block of lead for storage and shielding of the radioactive capsules and 12 vertical pneumatic tubes to maintain them in exposure position. These tubes form a cylindrical internal cavity with varying diameter between 10 and 84 cm. The isodose curves were determined for the geometries of 3, 6 and 12 actives tubes and 42 and 84 cm of diameter using the Fricke's chemical dosimeter. (Author) [pt

  5. Supplementary comparison CCRI(I)-S2 of standards for absorbed dose to water in 60Co gamma radiation at radiation processing dose levels

    DEFF Research Database (Denmark)

    Burns, D. T.; Allisy-Roberts, P. J.; Desrosiers, M. F.

    2011-01-01

    Eight national standards for absorbed dose to water in 60Co gamma radiation at the dose levels used in radiation processing have been compared over the range from 1 kGy to 30 kGy using the alanine dosimeters of the NIST and the NPL as the transfer dosimeters. The comparison was organized...... by the Bureau International des Poids et Mesures, who also participated at the lowest dose level using their radiotherapy-level standard for the same quantity. The national standards are in general agreement within the standard uncertainties, which are in the range from 1 to 2 parts in 102. Evidence of a dose...

  6. Comparison of the standards of absorbed dose to water of the VNIIFTRI, Russia and the BIPM for {sup 60}Co {gamma} rays

    Energy Technology Data Exchange (ETDEWEB)

    Allisy-Roberts, P.J.; Burns, D.T. [Bureau International des Poids et Mesures (BIPM), 92 - Sevres (France); Berlyand, V.; Bregadze, Y.; Korostin, S. [All-Russian Scientific Research Institute for Physical-Technical and Radiotechnical Measurements, Moscow (Russian Federation)

    2003-09-15

    A comparison of the standards of absorbed dose to water of the All-Russian Scientific Research Institute for Physical-Technical and Radio-technical Measurements (VNIIFTRI), Russia and of the Bureau International des Poids et Mesures (BIPM) has been made in {sup 60}Co gamma radiation. The results show that the VNIIFTRI and the BIPM standards for absorbed dose to water are in agreement, yielding a mean ratio of 0.9967 for the calibration factors of the transfer chambers, the difference from unity being within the combined standard uncertainty (0.0043) for this result. (authors)

  7. Comparison of the standards of air kerma of the OMH and the BIPM for 60Co gamma radiation

    International Nuclear Information System (INIS)

    Allisy-Roberts, P.J.; Kessler, C.; Burns, D.T.; Roger, P.; Machula, G.; Csete, I.; Rabus, H.

    2006-09-01

    A direct comparison between the standards for air kerma of the Orszagos Meresugyi Hivatal (OMH) and of the Bureau International des Poids et Mesures (BIPM) has been carried out in the 60 Co radiation beams of the BIPM. The result, expressed as a ratio of the OMH and the BIPM standards for air kerma, indicates a relative difference of 10.9 x 10 -3 with a combined standard uncertainty of 2.2 x 10 -3 . This new result agrees at the level of 0.4 x 10 -3 with the earlier direct comparisons performed in 1986 and 1994, as modified in 2001 by the application of wall and axial non-uniformity correction factors, calculated for the OMH standards using the Monte Carlo method. (authors)

  8. Studies on cannabis. III. Young plants from the seed irradiated with /sup 60/Co gamma rays for inhibiting their development after seeding

    Energy Technology Data Exchange (ETDEWEB)

    Shimomura, H; Kuriyama, E; Tomizawa, A [Tokyo Coll. of Pharmacy (Japan)

    1976-01-01

    The seedlings from Cannabis sativa L. seeds irradiated with different doses of ..gamma..-rays were examined, in order to determine the dose sufficient to kill the young plants naturally, before their hallucinnogenic component increases. The seeds of ''Minamioshihara No. 1'', which were harvested in 1972 in Tochigi Prefecture, were irradiated with eight different doses of /sup 60/Co ..gamma..-rays in January 17, 1973, and the seedlings were examined several times during the subsequent 9 months, from March to November 1973, and their morphological and histological effects were examined, and the results are summarized as follows: Samples irradiated with 1500 and 1000 krads developed radicles about 3 mm in length. Samples irradiated with 500, 200, and 50 krads grew into young plants with the first set of leaves, without lateral roots. Samples irradiated with 30 krads grew to about 10 cm high with a few lateral roots, and the epicotyls about 1 cm in length. These young plants from the irradiated seeds stayed in the same condition and then died. Samples irradiated with 15 and 5 krads grew in the same way as the controls until the stage of flowering. Samples irradiated with 500, 200, 50, and 30 krads showerd the cell membranes of endodermis and pericycle to be partially lignified and suberized. The degree of change was related to the dose of ..gamma..-rays. Samples irradiated with 30 krads showed withered cells near the end of the lateral nerves on the first and second set of leaves. The economical dose of /sup 60/Co ..gamma..-rays for inhibiting young plants from developing into adult ones was a minimum of 30 krads which made the young plants die. Irradiation with 50 krads of ..gamma..-rays will be required to kill the young plants completely before they develop the hallucinogenic component.

  9. Development of the 60Co gamma-ray standard field for therapy-level dosimeter calibration in terms of absorbed dose to water (ND,W)

    International Nuclear Information System (INIS)

    Fukumura, Akifumi; Mizuno, Hideyuki; Fukahori, Mai; Sakata, Suoh

    2013-01-01

    A primary standard for the absorbed dose rate to water in a 60 Co gamma-ray field was established at National Metrology Institute of Japan (NMIJ) in fiscal year 2011. Then, a 60 Co gamma-ray standard field for therapy-level dosimeter calibration in terms of absorbed dose to water was developed at National Institute of Radiological Sciences (NIRS) as a secondary standard dosimetry laboratory (SSDL). The results of an International Atomic Energy Agency (IAEA)/World Health Organization (WHO) TLD SSDL audit demonstrated that there was good agreement between NIRS stated absorbed dose to water and IAEA measurements. The IAEA guide based on the International Organization for Standardization (ISO) standard was used to estimate the relative expanded uncertainty of the calibration factor for a therapy-level Farmer type ionization chamber in terms of absorbed dose to water (N D,W ) with the new field. The uncertainty of N D,W was estimated to be 1.1% (k=2), which corresponds to approximately one third of the value determined in the existing air kerma field. The dissemination of traceability of the calibration factor determined in the new field is expected to diminish the uncertainty of dose delivered to patients significantly. (author)

  10. A comparison of the survival (LD/sub 50/30/) of a number of inbred mouse strains after X and 60Co gamma irradiation

    International Nuclear Information System (INIS)

    Vacha, J.; Znojil, V.; Hola, J.; Sikulova, J.

    1984-01-01

    The value of LD/sub 50/30/ for X and 60 Co gamma radiation was ascertained for several inbred mouse strains and the significance of interstrain differences in these parameters determined. The rank order of strains according to LD/sub 50/30/ differs as between the two types of radiation with the exception of the strains BALB/c and B10.LP/Ph, which are the least resistant to radiation-induced lethality with both types of radiation. The strain C57BL/10ScSnPh is highly resistant to X irradiation. The relative biological effectiveness of 60 Co gamma radiation as compared with X-radiation from the point of view of lethality fluctuates between 0.748 and 0.952 in individual strains, with a mean value of 0.866 +- 0.033. The RBE values do not correlate with the radiosensitivity of the strain, but they do correlate with the relative contribution to erythropoiesis of the spleen. (author)

  11. Growing, regeneration and radiosensibility of sugarcane callus (Saccharum spp. hybrid var. “SP 70-1284” treated with gamma radiation source 60Co.

    Directory of Open Access Journals (Sweden)

    Apolonio Valdez Balero

    2004-07-01

    Full Text Available Calluses in growth of sugar cane, variety “SP 70-1284”, were radiated with a source of 60Co. The studied doses were between 10 and 80 Gy. The affectation of the Gamma radiation source 60Co in the growth of the callus and the regeneration of plants was evaluated. The results indicated that the dose that diminished the growth of callus in a value under 50% was the one of 30 Gy, and also the dose that diminished the regenerative capacity of the callus below to the 50% of the population was the one of 30 Gy. The results showed that for this variety of sugar cane doses superior to 30Gy of Gamma radiations jeopardizes the growth and the later regeneration of the callus. Its application is recommended to induce genetic variability in the variety of sugar cane “SP 70-1284”, as well as in programs of genetic improvement by mutations, in susceptible varieties of the sugar cane red blight (Puccinia melanocephala Syd using calluses in growth. Key words: callus, hybrid, in vitro, improvement, rust, Saccharum sp, variability

  12. Calculation of direct effects of {sup 60}Co gamma rays on the different DNA structural levels: A simulation study using the Geant4-DNA toolkit

    Energy Technology Data Exchange (ETDEWEB)

    Tajik, Marjan; Rozatian, Amir S.H. [Department of Physics, University of Isfahan, Hezar Jarib Street, Isfahan 81746-73441 (Iran, Islamic Republic of); Semsarha, Farid, E-mail: Semsarha@ibb.ut.ac.ir [Institute of Biochemistry and Biophysics (IBB), University of Tehran, P.O. Box: 13145-1384, Tehran (Iran, Islamic Republic of)

    2015-03-01

    In this study, simple single strand breaks (SSB) and double strand breaks (DSB) due to direct effects of the secondary electron spectrum of {sup 60}Co gamma rays on different organizational levels of a volume model of the B-DNA conformation have been calculated using the Geant4-DNA toolkit. Result of this study for the direct DSB yield shows a good agreement with other theoretical and experimental results obtained by both photons and their secondary electrons; however, in the case of SSB a noticeable difference can be observed. Moreover, regarding the almost constant yields of the direct strand breaks in the different structural levels of the DNA, calculated in this work, and compared with some theoretical studies, it can be deduced that the direct strand breaks yields depend mainly on the primary double helix structure of the DNA and the higher-order structures cannot have a noticeable effect on the direct DNA damage inductions by {sup 60}Co gamma rays. In contrast, a direct dependency between the direct SSB and DSB yields and the volume of the DNA structure has been found. Also, a further study on the histone proteins showed that they can play an important role in the trapping of low energy electrons without any significant effect on the direct DNA strand breaks inductions, at least in the range of energies used in the current study.

  13. Comparison of the survival (LD/sub 50/30/) of a number of inbred mouse strains after X and /sup 60/Co gamma irradiation

    Energy Technology Data Exchange (ETDEWEB)

    Vacha, J.; Znojil, V.; Hola, J.; Sikulova, J. (Ceskoslovenska Akademie Ved, Brno. Biofysikalni Ustav)

    1984-01-01

    The value of LD/sub 50/30/ for X and /sup 60/Co gamma radiation was ascertained for several inbred mouse strains and the significance of interstrain differences in these parameters determined. The rank order of strains according to LD/sub 50/30/ differs as between the two types of radiation with the exception of the strains BALB/c and B10.LP/Ph, which are the least resistant to radiation-induced lethality with both types of radiation. The strain C57BL/10ScSnPh is highly resistant to X irradiation. The relative biological effectiveness of /sup 60/Co gamma radiation as compared with X-radiation from the point of view of lethality fluctuates between 0.748 and 0.952 in individual strains, with a mean value of 0.866 +- 0.033. The RBE values do not correlate with the radiosensitivity of the strain, but they do correlate with the relative contribution to erythropoiesis of the spleen.

  14. Effect of gamma radiation of {sup 60}Co in the variability of chinese bean [Vigna unguiculata (l.) Walpers] en R{sub 4}M{sub 4}; Efecto de la radiacion gamma de {sup 60}Co en la variabilidad de frijol chino [Vigna unguiculata (l.) Walpers] en R{sub 4}M{sub 4}

    Energy Technology Data Exchange (ETDEWEB)

    Salmeron E, J.; Bueno J, J.E.; Valencia E, F. [CSAEGro, Iguala, Guerrero, Tel and Fax 01 733 (33) 24328 (Mexico); Cervantes S, T. [IREGEP (Mexico); Cruz T, E. De la [ININ, Departamento de Biologia, 52750 La Marquesa, Estado de Mexico (Mexico)]. e-mail: csaegro@prodigy.net.mx

    2007-07-01

    Selection of plants of Chinese bean coming from seed irradiated with gammas of {sup 60}Co in the generation R{sub 4}M{sub 4} (fourth recurrent irradiation, fourth segregate generation) were carried out, taking as selection approaches the plant architecture, the numbers of sheaths for plant, sheath longitude, position of the sheaths, grain size and resistance to plagues and illnesses. 17 lines were selected for grain and 3 lines with fodder characteristics of black grain color were also obtained. (Author)

  15. Accumulation and dissipation of positive charges induced on a PMMA build-up cap of an ionisation chamber by 60Co gamma-ray irradiation

    International Nuclear Information System (INIS)

    Morishita, Y.; Takata, N.

    2013-01-01

    The signal current from an ionisation chamber with a PMMA build-up cap decreases with irradiation time due to electric fields produced by positive charges induced on the cap. In the present study, it was confirmed that the signal current decreases faster for irradiation using narrower 60 Co gamma-ray beams. This is because the number of secondary electrons that are emitted from surrounding materials and penetrate the build-up cap is smaller in a narrower gamma-ray beam, so that fewer positive charges are neutralised. The ionisation chamber was first subjected to continuous gamma-ray irradiation for 24 h, following which it was irradiated with shorter periodic gamma-ray bursts while measuring the current signal. This allowed the coefficients of positive charge accumulation and dissipation to be determined. It was found that the dissipation coefficient has a large constant value during gamma-ray irradiation and decreases asymptotically to a small value after irradiation is stopped. From the coefficients, the minimum signal current was calculated, which is the value when accumulation and dissipation balance each other under continuous irradiation. The time required for the signal current to recover following irradiation was also calculated. (authors)

  16. Effect of "6"0Co-induced gamma radiation exposure and Jasmonic acid on antioxidant responses in Cowpea varieties

    International Nuclear Information System (INIS)

    Shukla, Pradeep Kumar; Bhagoji, Ravi; Ramteke, P.W.; Misra, Pragati; Maurice, Navodita

    2017-01-01

    Cowpea used to be the first crop harvested before the cereal crops are ready and therefore is referred to as 'hungry-season crop'. The gamma irradiation is known to increase nutritional values of food sources and also enhance and accelerate growth of certain vegetables Antioxidants are the metabolites, produced by the plant in response to different stress conditions e.g. radiation stress etc. Seeds of four cowpea varieties were treated with different doses of gamma radiation and effect of jasmonic acid (JA) on antioxidant response of cowpea was studied. The results showed that there was no linear correlation between application of JA and different antioxidant contents. (author)

  17. Study of the action of 60Co gamma radiation on Salmonella poona, Escherichia coli and Alicyclobacillus acidoterrestris in mango pulp

    International Nuclear Information System (INIS)

    Pereira, Marco Antonio dos Santos

    2009-01-01

    The application of non-thermal treatments has proven effective in inhibiting bacteria such as Salmonella and Escherichia coli. Mango is a fruit of national consumption with a great exportation potential. Meanwhile, outbreaks of food borne disease related to mango consumption caused mistrust on the degree of food security offered by the product. The objective of this work was to establish the radioresistance of bacteria Escherichia coli, Salmonella Poona and Alicyclobacillus acidoterrestris on the mango pulp by the calculation of the D10 values and to know the radiation effect on the sensory characteristics of the fruit pulp. The microbiological profile of frozen mango pulp available at the local market was also established using conventional methods of plating and Most Probable Number (MPN). The pulps experimentally inoculated with the bacteria listed above were irradiated with doses of 0, 1, 2, 3, 4 and 5kGy in a 60 Co source. The sensory analysis was performed using a dose of 5 kGy, using the triangular test and the test of acceptance with hedonic scale. The results of this study show that the quality of mango pulp sell in the local market is not satisfactory in accordance with the standards established by the Brazilian law and the literature, showing the need of using other tools to achieve acceptable levels of quality. The D10 values obtained are in the range of 1.01 and 1.09kGy for E. coli ATCC 8739, 0.60 and 0.98kGy for S. poona and 0.72 e 0.88kGy for A. acidoterrestris respectively. The triangular test showed that a 5kGy radiation dose changed the sensory characteristics of mango pulp. Nevertheless, sensory analysis of a food product prepared with the irradiated pulp obtained good acceptance in the attributes of global appearance, flavor and aroma. (author)

  18. Study of the action of 60Co gamma radiation on Salmonella poona, Escherichia coli and Alicyclobacillus acidoterrestris in mango pulp

    International Nuclear Information System (INIS)

    Pereira, Marco Antonio dos Santos

    2009-01-01

    The application of non-thermal treatments has proven effective in inhibiting bacteria such as Salmonella and Escherichia coli. Mango is a fruit of national consumption with a great exportation potential. Meanwhile, outbreaks of foodborne disease related to mango consumption caused mistrust on the degree of food security offered by the product. The objective of this work was to establish the radioresistance of bacteria Escherichia coli, Salmonella Poona and Alicyclobacillus acidoterrestris on the mango pulp by the calculation of the D10 values and to know the radiation effect on the sensory characteristics of the fruit pulp. The microbiological profile of frozen mango pulp available at the local market was also established using conventional methods of plating and Most Probable Number (MPN). The pulps experimentally inoculated with the bacteria listed above were irradiated with doses of 0, 1, 2, 3, 4 and 5 kGy in a 60 Co source. The sensory analysis was performed using a dose of 5 kGy, using the triangular test and the test of acceptance with hedonic scale. The results of this study show that the quality of mango pulp sell in the local market is not satisfactory in accordance with the standards established by the Brazilian law and the literature, showing the need of using other tools to achieve acceptable levels of quality. The D10 values obtained are in the range of 1.01 and 1.09 kGy for E. coli ATCC 8739, 0.60 and 0.98 kGy for S. poona and 0.72 and 0.88 kGy for A. acidoterrestris respectively. The triangular test showed that a 5 kGy radiation dose changed the sensory characteristics of mango pulp. Nevertheless, sensory analysis of a food product prepared with the irradiated pulp obtained good acceptance in the attributes of global appearance, flavor and aroma. (author)

  19. {sup 60}Co {gamma} irradiation effects on the current-voltage (I-V) characteristics of Al/SiO{sub 2}/p-Si (MIS) Schottky diodes

    Energy Technology Data Exchange (ETDEWEB)

    Tataroglu, A. [Department of Physics, Faculty of Arts and Sciences, Gazi University 06500, Ankara (Turkey)]. E-mail: ademt@gazi.edu.tr; Altindal, S. [Department of Physics, Faculty of Arts and Sciences, Gazi University 06500, Ankara (Turkey); Buelbuel, M.M. [Department of Physics, Faculty of Arts and Sciences, Gazi University 06500, Ankara (Turkey)

    2006-12-01

    It is well known that the exposure of any semiconductor surfaces to the {sup 60}Co {gamma}-ray irradiation causes electrically active defects. To investigate the effect of {gamma}-ray irradiation dose on the electrical characteristics of metal-insulator-semiconductor (MIS) Schottky diodes, the fabricated devices were exposed to {gamma} radiation at a dose of 2.12 kGy/h. The total dose range was from 0 to 450 kGy at room temperature. The density of interface states N {sub ss} as a function of E {sub ss}-E {sub v}, the values of series resistance R {sub s} and the bias dependence of the effective barrier height {phi} {sub e} for each dose were obtained from the forward bias I-V characteristics. Experimental results show that the {gamma}-irradiation gives rise to an increase in the zero bias barrier height {phi} {sub BO}, as the ideality factor n, R {sub s} and N {sub ss} decreases with increasing radiation dose.

  20. Effect of gamma radiation (60Co) on the organoleptic and nutritive properties of common beans (Phaseolus vulgaris L., cv. mulatinho)

    International Nuclear Information System (INIS)

    Fonseca, H.; Nogueira, J.N.; Maffei, C.I.; Oliveira, M.G.

    1974-05-01

    The study of the influence of gamma radiation on the organoleptic and nutritive properties of kidney beans is presented. Samples of this vegetable were irradiated with 15 krad and stored for five months. Immediately after irradiation and monthly, sensory evaluations and chemical analysis of the vitamins thiamin (B1) and riboflavin (B2) were accomplished in the samples. The results showed an evident influence of radiation during its application since the higher losses occurred immediately after this treatment, mainly in riboflavin, which from the total losses observed, 47,9% occurred during irradiation. The riboflavin is much more sensitive to gamma radiation and in terms of organoleptic properties it was observed the development of an undesirable flavor and a hardening of the texture in the irradiated samples. However, at the end of the experiment no difference was found between control and irradiated samples

  1. Study of 60 Co gamma radiation effects on the biochemical, biological and immunological properties of the Bothrops jararaca venom

    International Nuclear Information System (INIS)

    Guarnieri, M.C.

    1992-01-01

    Gamma radiation, by including different modifications on the toxic, enzymatic and immunological activities of proteins, could be an useful implement for detoxification of snake venoms. The present work was done to study the mechanism of action and effects of gamma rays on the Bothrops jararaca venom, determining the radiation dose that attenuates the toxic and enzymatic activities maintaining the immunological properties of venom, and also the most important free radicals on this process. The results of immuno diffusion, immunoblotting, immunoprecipitation, immunization of mice and rabbits, and neutralization tests, showed the maintenance of antigenic and immunogenic properties and decrease of neutralizing capacity of antibodies induced by 3,000 and 4,000 Gy irradiated venom. Since the immunological properties were the most radioresistant, it was possible to determine the dose of 2,000 Gy, as the ideal radiation dose in the treatment of venoms aiming the improvement of the immunization schedule to obtain bothropic antisera. (author). 164 refs, 19 tabs, 54 figs

  2. Structural and optical analysis of 60Co gamma-irradiated thin films of polycrystalline Ga10Se85Sn5

    Science.gov (United States)

    Ahmad, Shabir; Asokan, K.; Shahid Khan, Mohd.; Zulfequar, M.

    2015-12-01

    The present study focuses on the effects of gamma irradiation on structural and optical properties of polycrystalline Ga10Se85Sn5 thin films with a thickness of ∼300 nm deposited by the thermal evaporation technique on cleaned glass substrates. X-ray diffraction patterns of the investigated thin films show that crystallite growth occurs in the orthorhombic phase structure. The surface study carried out by using the scanning electron microscope (SEM) confirms that the grain size increases with gamma irradiation. The optical parameters were estimated from optical transmission spectra data measured from a UV-vis-spectrophotometer in the wavelength range of 200-1100 nm. The refractive index dispersion data of the investigated thin films follow the single oscillator model. The estimated values of static refractive index n0, oscillator strength Ed, zero frequency dielectric constant ε0, optical conductivity σoptical and the dissipation factor increases after irradiation, while the single oscillator energy Eo decreases after irradiation. It was found that the value of the optical band gap of the investigated thin films decreases and the corresponding absorption coefficient increases continuously with an increase in the dose of gamma irradiation. This post irradiation changes in the values of optical band gap and absorption coefficient were interpreted in terms of the bond distribution model.

  3. Protective role of Hippopahe leaves against kidney damage in total body 60Co-gamma-irradiated mice

    International Nuclear Information System (INIS)

    Saini, Manu; Prasad, Jagdish; Bala, Madhu

    2012-01-01

    Hippophae rhamnoides has diverse therapeutic applications in Indian, Chinese and Tibetan medicine. Our earlier studies have shown that pretreatment with Hippophae leaf extract rendered >90% survival in mice population. The objective of this study was to investigate the role of our herbal preparation from Hippophae leaf against radiation induced kidney damage. The study was conducted with Strain 'A' male mice weighing approximately 28 ±2 g. The mice were administered Hippophae leaf extract, 30 minutes prior to 60 cobalt-gamma irradiation. The weight of kidneys and histological changes in kidney tissues at the light microscopic level were examined at 2, 5, 7, 10 and 15 days after treatment. The results showed that no significant change was observed in kidney weight after 60 cobalt-gamma irradiation. The glomerular damage in the form of glomerular sclerosis and percentage of damaged glomeruli; tubular damage in form of tubular dilations; apoptosis, and interstitial hemorrhages in renal cortex was also observed after radiation treatment. The pretreatment with Hippophae leaf extract countered most of the histological alterations induced by radiation. In comparison to radiation alone group, there was a significant decrease (p 60 cobalt gamma radiation induced damage. (author)

  4. Effect of 60Co gamma radiation on insects infesting rice during storage. Part III. Oryzaephylus surinamensis L., Cryptolestes pusillus S. and Cryptolestes ferrugineus S. (Cucujidae; Coleoptera)

    International Nuclear Information System (INIS)

    Lorenzo, P.

    1997-01-01

    Losses in rice grain in quantity and quality during storage are caused by insects infestations. In this paper the effect of 60 Co gamma radiation on the mortality of Oryzaephilus surinamensis L., Cryptolestes pusillus S. and Cryptolestes ferrugineus S. adults was study. Insects were irradiated with doses from 0,20 to 1,00 kGy at dose rates from 0,97 to 0,94 kGy.h -1 . After the treatment, the radiation damages were evaluated taking in consideration the sterility and mortality produced in the irradiated populations. According to the experimental results it was confirmed that irradiation doses from 0,40 to 0,60 kGy were effective to control the insects pests of stored rice in Cuba [es

  5. Comparison of the standards for absorbed dose to water of the VNIIFTRI, Russia and the BIPM in {sup 60}Co gamma rays

    Energy Technology Data Exchange (ETDEWEB)

    Allisy-Roberts, P.J.; Kessler, C.; Burns, D.T. [Bureau International des Poids et Mesures (BIPM), 92 - Sevres (France); Berlyand, V.; Berlyand, A. [All-Russian Scientific Research Institute for Physical-Technical and Radiotechnical Measurements, Moscow (Russian Federation)

    2010-02-15

    A new comparison of the standards for absorbed dose to water of the All-Russian Scientific Research Institute for Physical-Technical and Radio-technical Measurements (VNIIFTRI), Russia and of the Bureau International des Poids et Mesures (BIPM) has been made in {sup 60}Co gamma radiation in 2009. The results show that the VNIIFTRI and the BIPM standards for absorbed dose to water are in agreement, yielding a mean ratio of 0.9976 for the calibration coefficients of the transfer chambers, the difference from unity being within the combined standard uncertainty (0.0043) for this result. This result is consistent with the earlier 2001 comparison result of 0.9967 (43). The updated degrees of equivalence for the VNIIFTRI are compared with those of the other national metrology institutes as presented in the BIPM key comparison database. (authors)

  6. Effect of 60Co gamma radiation on the performance of two soybean varieties in M1 and M2 generations

    International Nuclear Information System (INIS)

    Miah, M.A.; Rahman, L.

    1982-01-01

    Seed of two soybean varieties Bragg and Improved Pelican were treated with gamma-rays at 0, 11, 29, 47 and 65 Kr doses. Percentage of seedling emergence in both varieties in M 1 generation was not so seriously affected as in M 1 generation. The plant height of Bragg after one month of sowing was less influenced than Improved Pelican. Plant height of both the varieties decreased with increase in radiation doses; except 11 Kr in Bragg in both generations. Similar results were also obtained in plant height at harvest except that Improved Pelican in M 1 generation produced taller plants at 29 Kr than at 11 Kr level. Days required to harvest increased at higher doses in comparison to control, except 11 Kr for Improved Pelican and 29 Kr for Bragg in M 1 generation. (author)

  7. Cumulative dose 60Co gamma irradiation effects on AlGaN/GaN Schottky diodes and its area dependence

    Science.gov (United States)

    Sharma, Chandan; Laishram, Robert; Rawal, Dipendra Singh; Vinayak, Seema; Singh, Rajendra

    2018-04-01

    Cumulative dose gamma radiation effects on current-voltage characteristics of GaN Schottky diodes have been investigated. The different area diodes have been fabricated on AlGaN/GaN high electron mobility transistor (HEMT) epi-layer structure grown over SiC substrate and irradiated with a dose up to the order of 104 Gray (Gy). Post irradiation characterization shows a shift in the turn-on voltage and improvement in reverse leakage current. Other calculated parameters include Schottky barrier height, ideality factor and reverse saturation current. Schottky barrier height has been decreased whereas reverse saturation current shows an increase in the value post irradiation with improvement in the ideality factor. Transfer length measurement (TLM) characterization shows an improvement in the contact resistance. Finally, diodes with larger area have more variation in the calculated parameters due to the induced local heating effect.

  8. Ambient-temperature diffusion and gettering of Pt atoms in GaN with surface defect region under 60Co gamma or MeV electron irradiation

    Science.gov (United States)

    Hou, Ruixiang; Li, Lei; Fang, Xin; Xie, Ziang; Li, Shuti; Song, Weidong; Huang, Rong; Zhang, Jicai; Huang, Zengli; Li, Qiangjie; Xu, Wanjing; Fu, Engang; Qin, G. G.

    2018-01-01

    Generally, the diffusion and gettering of impurities in GaN needs high temperature. Calculated with the ambient-temperature extrapolation value of the high temperature diffusivity of Pt atoms in GaN reported in literature, the time required for Pt atoms diffusing 1 nm in GaN at ambient temperature is about 19 years. Therefore, the ambient-temperature diffusion and gettering of Pt atoms in GaN can hardly be observed. In this work, the ambient-temperature diffusion and gettering of Pt atoms in GaN is reported for the first time. It is demonstrated by use of secondary ion mass spectroscopy that in the condition of introducing a defect region on the GaN film surface by plasma, and subsequently, irradiated by 60Co gamma-ray or 3 MeV electrons, the ambient-temperature diffusion and gettering of Pt atoms in GaN can be detected. It is more obvious with larger irradiation dose and higher plasma power. With a similar surface defect region, the ambient-temperature diffusion and gettering of Pt atoms in GaN stimulated by 3 MeV electron irradiation is more marked than that stimulated by gamma irradiation. The physical mechanism of ambient-temperature diffusion and gettering of Pt atoms in a GaN film with a surface defect region stimulated by gamma or MeV electron irradiation is discussed.

  9. Description of morphologic characters in avocado trees (Persea americana. Mill) var. Hass, submitted to gamma radiation of 60 Co

    International Nuclear Information System (INIS)

    Sandoval R, L.

    2004-01-01

    It was carried out the characterization of 77 trees of avocado ''Hass'' subjected to gamma radiation of Co 60 , in doses of 0, 5, 10,15, 20 and 25 Gy based on previous studies of radiosensitivity with ends of improvement located in the experimental center ''La Labor'' belonging to the foundation Salvador Sanchez Colin. CICTAMEX. S C. in Temascaltepec, Mexico. It was carried out the morphological characterization of this variety according to the nomenclature settled down by the International Office of Genetic Resources (IBPGRI), registering 27 variables for the description of the tree, 33 for description of the fruit and 14 for the characterization of the seed. Regarding the architectural characterization 10 variables were evaluated. Using gamma radiation of Co 60 in the doses of 15 and 20 Gy it was detected a reduction of 22% in the portage of the tree, likewise had reduction the diameter of the trunk and of the foliage. It was found that the reduction in the behavior of the tree induces to a bigger variation of the form of the same one, in the distribution of the branches in the form of the fruit and in the form of the seed you Also detects a remarkable increment in the productivity of some hoist irradiated regarding the architecture of the tree one observes that the angle of inclination of the branches increase when being increased the radiation dose and that the longitude of the annual section of growth decreased significantly in the doses of 20 and 25 Gy. One observes a significant Increment in the I number of buds prolepticos in those hoist irradiated in the I finish mbdulo of rhythmic growth. Io that indicates a change substantial in the I inhabit of growth With the purpose of determining if the changes in morphology and architecture of the tree corresponded with the modification at level meristematico, they were carried out observations to the court microscope of having knitted apical vegetative meristematico in samples of you hoist of all the treatments

  10. Temperature dependence of luminescence from silica glasses under in-reactor and 60Co gamma-ray irradiation

    Science.gov (United States)

    Takahara, Shogo; Yoshida, Tomoko; Tanabe, Tetuo; , Tatuya, Ii; Hirano, Masahiro; Okada, Moritami

    2004-06-01

    In order to investigate the temperature effects on the dynamic radiation damaging process, we have carried out in situ measurements of in-reactor luminescence (IRL) and gamma-ray induced luminescence (GIL) of a silica glass at temperatures ranging from 70 K to 370 K. Both luminescence spectra were found to consist of two broad emission centers at 3.1 eV and 4.1 eV with an additional temperature independent emission around 2.5 eV. The 2.5 eV emission different from the other two showed long tail to the lower energy side and was attributed to the Cherenkov radiation. The 3.1 eV band was attributed to a B 2 β oxygen deficient center on the basis of our photoluminescence measurement. The intensity of the 3.1 eV IRL increased with increasing temperature up to ca. 200 K and saturated above 200 K, which is clearly different from the reported temperature dependence of 3.1 eV photoluminescence, suggesting the existence of some different relaxation mechanism of excited electron under ionizing radiations.

  11. Effects of 60Co gamma radiation on toxicity and hemorrhagic, myonecrotic, and edema-forming activities of Cerastes cerastes venom

    International Nuclear Information System (INIS)

    Abib, H.; Laraba-Djebari, F.

    2003-01-01

    Antisera are used as effective antidotes against the local effects of snake bites. To improve antisera production and extend the life of surrogates used to produce antibodies, the chronic effects of venom toxicity must be reduced. The present study evaluated the effectiveness of gamma irradiation to reduce the local effects associated with viperid snake bites by evaluating in NMRI mice the toxicity and edematic, hemorrhagic, and myonecrotic activities of native and irradiated Cerastes cerastes venoms. These results indicated that the toxicity of irradiated venoms (1 and 2 kGy) decreased as compared with that of native venom. The edematic and hemorrhagic activities were also reduced in the detoxified samples, particularly with the 2-kGy radiation dose. Furthermore, the creatine phosphokinase (CPK) activity was significantly increased in the serum and decreased in the myocardium after envenomation with native venom, but no significant enzymatic changes were observed in mice envenomated with irradiated venom. Histopathologic evaluation showed that native venom caused severe degenerative changes in the myocardium. In the case of 2-kGy-irradiated venom, no tissue alterations were observed. These results indicate that irradiation of venom with a 2-kGy dose may offer an effective method for reducing the chronic toxic effects of venom in immunized animals. (author)

  12. Different gamma radiation doses effects of 60 Co on buds of banana plant (Nanicao-AAA) breeded in vitro

    International Nuclear Information System (INIS)

    Alves, Gilberto Dias; Colaco, Waldeciro

    1999-01-01

    Buds from banana cv. Nanicao-AAA (3 mm x 3 mm) were aseptically cultured in a modified Murashige and Skoog (MS) basal medium supplement with bezylaminepurine and sucrose, and solidified with agar. Buds placed on sterile Petri dishes were irradiated with increasing gamma rays doses (10, 20, 30, and 40 Gy). The statistical design was completely randomized with 5 doses and 30 replications. After irradiation, buds were transferred to 10 ml of the same medium into test tubes, and allowed to grow in a controlled environment (25 deg C, 16 h illumination ) for 4 weeks. There were no significant differences (Tukey, 0,05) between doses in terms of oxidation: in around 20% for all treatments. On the other hand, a statistically significant decrease in the germination of new buds with increased doses of irradiation was observed. The treatment with 40 Gy reduced in 80% the germination of new buds by the end of the evaluated period (4 weeks), resulting in a mean production of 1,5 buds. Mean production in the control was 7,6 buds. no statistically significant differences were detected between treatments with 10, 20, and 30 Gy, with a mean production of 3 buds, less than half obtained in the control. (author)

  13. Study about the sensibility in vitro of different strains of Vibrio cholera 01 exposed to 60 Co gamma radiation; Estudo da sensibilidade in vitro de diferentes cepas de Vibio cholerae 01 a radiacao gama de 60Co

    Energy Technology Data Exchange (ETDEWEB)

    Moraes, Ivany Rodrigues de [Instituto Adolfo Lutz, Sao Paulo, SP (Brazil). Servicos de Saude; Gelli, Dilma Scala; Jakabi, Miyoko [Instituto Adolfo Lutz, Sao Paulo, SP (Brazil). Secao de Microbiologia; Mastro, Nelida Lucia del [Instituto de Pesquisas Energeticas e Nucleares (IPEN), Sao Paulo, SP (Brazil)]. E-mail: nlmastro@net.ipen.br

    1998-07-01

    The presence of some microorganisms in food, or the metabolites originated during their own multiplication may bring several diseases to humans: intoxications and food borne infections. Among the agents that may cause those diseases, we find Vibrio cholerae 01. In this experiment, the studies are focused on the radiosensibility in vitro of four strains of V. cholerae 01, exposed to different doses of ionizing radiation of {sup 60} Co. The results are compared with other data related to bacterial food borne diseases, including water. (author)

  14. Gamma (60CO) radiation effects on arcelin protein and evaluation of bean lineages against Acanthoscelides obtectus (Say) and Zabrotes subfasciatus (Boh.) (Col.: Bruchidae)

    International Nuclear Information System (INIS)

    Botelho, Ana Claudia Girardo

    2006-01-01

    The resistance of arcelin carrying seeds of bean lineages (Phaseolus vulgaris L.) against the bean weevils Acanthoscelides obtectus (Say, 1831) and Zabrotes subfasciatus (Bohemann, 1833) (Coleoptera: Bruchidae), and the influence of gamma radiation ( 60 CO) on the manifestation of arcelin resistance to Z. subfasciatus were verified. Laboratorial tests, in choice and non-choice tests, with wild specimens carrying Arc-1, Arc-2, Arc-3, Arc-4, Raz-56 and Raz-59 (with Arc-5 alleles) and commercial lineages as control IAC - Carioca and IAC - Arua were conducted. Statistical design was completely randomized, with five repetitions, with 10 g of grains from each lineage samples by portion. Attractiveness, oviposition, emergence, mortality, adults' weigh and longevity, developing period, sexual rate, seeds' weigh loss, infestation and fecundity (Z. subfasciatus) were observed. Gamma radiation doses irradiations, in general, haven't affected the resistance manifestation of lineages carrying arcelin protein variants against the Z. subfasciatus bean weevil, thus, joint application use of both control methods can be recommended. Raz-56 lineage showed high resistance of the antibiosis types and non-preference for oviposition and feeding to Z. subfasciatus, while Raz-59 showed antibiosis and non-preference for feeding, and both (Raz-56 and Raz-59) showed intermediate resistance to A. obtectus, against which lineage Arc-2 was the most harmful to its development, expressing non-preference to feeding and/or antibiosis. (author)

  15. The effect of {sup 60}Co ({gamma}-ray) irradiation on the electrical characteristics of Au/SnO{sub 2}/n-Si (MIS) structures

    Energy Technology Data Exchange (ETDEWEB)

    Goekcen, M. [Physics Department, Faculty of Arts and Sciences, Gazi University, 06500 Teknikokullar, Ankara (Turkey); Tataroglu, A. [Physics Department, Faculty of Arts and Sciences, Gazi University, 06500 Teknikokullar, Ankara (Turkey)], E-mail: ademt@gazi.edu.tr; Altindal, S.; Buelbuel, M.M. [Physics Department, Faculty of Arts and Sciences, Gazi University, 06500 Teknikokullar, Ankara (Turkey)

    2008-01-15

    The effect of {sup 60}Co ({gamma}-ray) irradiation on the electrical properties of Au/SnO{sub 2}/n-Si (MIS) structures has been investigated using the capacitance-voltage (C-V) and conductance-voltage (G/{omega}-V) measurements in the frequency range 1 kHz to 1 MHz at room temperature. The MIS structures were exposed to {gamma}-rays at a dose rate of 2.12 kGy/h in water and the range of total dose was 0-500 kGy. It was found that the C-V and G/{omega}-V curves were strongly influenced with both frequency and the presence of the dominant radiation-induced defects, and the series resistance was increased with increasing dose. Also, the radiation-induced threshold voltage shift ({delta}V{sub T}) strongly depended on radiation dose and frequency, and the density of interface states N{sub ss} by Hill-Coleman method decreases with increasing radiation dose.

  16. Effects of gamma irradiation of the 60 Co on antimicrobial action of plant extracts of bark and leaves of Schinus terebinthifolius Raddi

    International Nuclear Information System (INIS)

    Silva, Edvane Borges da; Santos, Gustavo Henrique Farias dos; Amaral, Ademir de Jesus; Xisto, Kesia; Araujo, Rosilma de Oliveira

    2014-01-01

    This study aimed to evaluate the potential for antimicrobial activity in vitro of extracts of bark and leaves of S. terebinthifolius treated with 60 Co gamma radiation. 5,0 doses were used; 7.5 and 10 kGy, being held non-irradiated controls. To determine the antimicrobial activity was applied to the disc diffusion technique to evaluate the diameter of the inhibition zones against Gram-positive bacteria, gram-negative bacteria, alcohol-acid-resistant and yeast. Antimicrobial activity was considered significant for halos ≥ 15 mm. The results indicate an intensification of antimicrobial action of bark extracts, the 5.0 kGy, against S. aureus. Was held the micro dilution in broth to determine the minimum inhibitory concentration (MIC) and Minimum Bactericidal Concentration (MBC) of peels extracts, compared to eight clinical isolates of S. aureus. The MBC values showed that ionizing radiation did not produce the increased of anti bacteriostatic action of S. terebinthifolius, but the results indicated that S. terebinthifolius bark extracts can be used as an antimicrobial agent and ionizing radiation as an important alternative in this conservation feature

  17. Damage to plasmid DNA produced by 60Co-gamma radiation and subsequent repair processes in E. coli with and without SOS induction

    International Nuclear Information System (INIS)

    Bien, M.

    1986-01-01

    This study was carried out to provide information on the question as to whether radiation-induced separation of double-stranded DNA in E. coli is followed by repair processes leading to the formation of replicable material. For the detection of those double-strand breaks, E. coli was first transformed using enzymatically linearised dBR 322-DNA. This served as a reference standard to compare the transformations using radiated DNA. DNA was either exposed to increasing doses of 60 Co-gamma radiation or separated into one oc-fraction and one lin-fraction following exposure to 30 Gy. The DNA samples thus obtained were then used to transform three different strains of E. coli (wild strain, SFX, SFXrecA - ). In order to improve the repair yield, the cells were additionally SOS-induced using ultraviolet radiation. The mutation rates were a measure of the number of errors occurring during the various repair processes. Restriction analysis was carried out to characterise the resulting mutants in greater detail. (orig./MG) [de

  18. Activity evaluation from different native or irradiated with 60 Co gamma rays snake venoms and their inhibitory effect on Leishmania (Leishmania) amazonensis

    International Nuclear Information System (INIS)

    Lourenco, Cecilia de Oliveira

    2000-01-01

    Cutaneous leishmaniasis is a disease, caused by Leishmania parasites, that occurs frequently in tropical and sub-tropical regions of the world. Skin lesions that could results in disfiguring aspect characterize it. The treatment is based on few drugs as antimony salts or pentamidine that are toxic with increasing resistance by the parasite. Alternative forms of disease treatment are in constant search, including natural components as snake venoms. Previous studies demonstrate that some components of snake venoms have an inhibitory effect against those parasites, including Leishmania species. Although snake venoms presented high toxicity, several methods have been described to detoxify most or some of their toxic components, with favorable results by the use of gamma irradiation. In this report we tested several native and irradiated snake venoms for inhibitory effect against Leishmania (Leishmania) amazonensis parasite and LLCMK 2 mammalian cells, with enzymatic tests and electrophoresis. There are significant activity in Acanthophis antarcticus, Agkistrodon bilineatus, Bothrops moojeni, Bothrops jararaca, Hoplocephalus stephensi, Naja melanoleuca, Naja mossambica, Pseudechis australis, Pseudechis colletti, Pseudechis guttatus and Pseudechis porphyriacus, venom being inactive Pseudonaja textilis, Notechis ater niger, Notechis scutatus. Oxyuranus microlepidotus and Oxyuranus scutellatus venoms. After 2 KGy of 60 Co irradiation most venom loses significantly their activity. Venoms with antileishmanial activity presented L-amino acid oxidase (L-AO) activity and showed common protein with a molecular weight about 60kDa in SDS-PAGE. These results indicate that L-AO activity in those venoms are probably related with antileishmanial effect. (author)

  19. Optimization of process parameters for the inactivation of Lactobacillus sporogenes in tomato paste with ultrasound and {sup 60}Co-{gamma} irradiation using response surface methodology

    Energy Technology Data Exchange (ETDEWEB)

    Ye Shengying [College of Food Science, South China Agricultural University, Wushan, Guangzhou, GD 510640 (China)], E-mail: yesy@scau.edu.cn; Qiu Yuanxin; Song Xianliang; Luo Shucan [College of Food Science, South China Agricultural University, Wushan, Guangzhou, GD 510640 (China)

    2009-03-15

    The processing parameters for ultrasound and {sup 60}Co-{gamma} irradiation were optimized for their ability to inactivate Lactobacillus sporogenes in tomato paste using a systematic experimental design based on response surface methodology. Ultrasonic power, ultrasonic processing time and irradiation dose were explored and a central composite rotation design was adopted as the experimental plan, and a least-squares regression model was obtained. The significant influential factors for the inactivation rate of L. sporogenes were obtained from the quadratic model and the t-test analyses for each process parameter. Confirmation of the experimental results indicated that the proposed model was reasonably accurate and could be used to describe the efficacy of the treatments for inactivating L. sporogenes within the limits of the factors studied. The optimized processing parameters were found to be an ultrasonic power of 120 W with a processing time of 25 min and an irradiation dose of 6.5 kGy. These were measured under the constraints of parameter limitation, based on the Monte Carlo searching method and the quadratic model of the response surface methodology, including the a/b value of the Hunter color scale of tomato paste. Nevertheless, the ultrasound treatment prior to irradiation for the inactivation of L. sporogenes in tomato paste was unsuitable for reducing the irradiation dose.

  20. Fricke dosimetry: the difference between G(Fe3+) for 60Co gamma-rays and high-energy x-rays.

    Science.gov (United States)

    Klassen, N V; Shortt, K R; Seuntjens, J; Ross, C K

    1999-07-01

    A calibration of the Fricke dosimeter is a measurement of epsilon G(Fe3+). Although G(Fe3+) is expected to be approximately energy independent for all low-LET radiation, existing data are not adequate to rule out the possibility of changes of a few per cent with beam quality. When a high-precision Fricke dosimeter, which has been calibrated for one particular low-LET beam quality, is used to measure the absorbed dose for another low-LET beam quality, the accuracy of the absorbed dose measurement is limited by the uncertainty in the value of G(Fe3+). The ratio of G(Fe3+) for high-energy x-rays (20 and 30 MV) to G(Fe3+) for 60Co gamma-rays, G(Fe3+)MV(Co), was measured to be 1.007(+/-0.003) (confidence level of 68%) using two different types of water calorimeter, a stirred-water calorimeter (20 MV) and a sealed-water calorimeter (20, 30 MV). This value is consistent with our calculations based on the LET dependence of G(primary products) and, as well, with published measurements and theoretical treatments of G(Fe3+).

  1. A comparison of Australian and Canadian calibration coefficients for air kerma and absorbed dose to water for 60Co gamma radiation.

    Science.gov (United States)

    Shortt, K R; Huntley, R B; Kotler, L H; Boas, J F; Webb, D V

    2006-06-01

    Australian and Canadian calibration coefficients for air kerma and absorbed dose to water for 60Co gamma radiation have been compared using transfer standard ionization chambers of types NE 2561 and NE 2611A. Whilst the primary standards of air kerma are similar, both being thick-walled graphite cavity chambers but employing different methods to evaluate the Awall correction, the primary standards of absorbed dose to water are quite different. The Australian standard is based on measurements made with a graphite calorimeter, whereas the Canadian standard uses a sealed water calorimeter. The comparison result, expressed as a ratio of calibration coefficients R=N(ARPANSA)/N(NRC), is 1.0006 with a combined standard uncertainty of 0.35% for the air kerma standards and 1.0052 with a combined standard uncertainty of 0.47% for the absorbed dose to water standards. This demonstrates the agreement of the Australian and Canadian radiation dosimetry standards. The results are also consistent with independent comparisons of each laboratory with the BIPM reference standards. A 'trilateral' analysis confirms the present determination of the relationship between the standards, within the 0.09% random component of the combined standard uncertainty for the three comparisons.

  2. Cytotoxicity evaluation of Diethyltoluamide (DEET) in Perna perna (Linnaeus, 1758) mussels non-irradiated and irradiated with 60Co gamma radiation

    International Nuclear Information System (INIS)

    Martini, Gisela de Assis

    2013-01-01

    Recent studies have identified the presence of several emerging pollutants in aquatic environments. The occurrence in different environmental matrices has been continuously reported, highlighting the need for toxicity studies. The DEET (N,N-diethyl-meta-toluamide) is the active ingredient used in most insect repellents, and is present in many commercially available formulations. Apart from chemical pollutants, aquatic organisms are subject to exposure of ionizing radiation from natural sources or in the vicinity of nuclear power plants. The present study evaluated the toxicity of DEET in organisms irradiated and non-irradiated with 60 Co gamma radiation, and the effects that radiation causes in lysosomes of hemocytes of Perna perna mussel. For this purpose, assays were performed to identify the acute toxicity of DEET concentration and the dose of gamma radiation able to cause mortality. Subsequently, cytotoxicity assays were carried out to assess the stability of the lysosomal membrane in organisms exposed to ionizing radiation and DEET. According to the results obtained in acute toxicity tests, the concentration of DEET that causes mortality of 50% exposed organisms (LC50) is 114,27 mg L -1 , and the radiation dose that causes mortality (LD50) is 1068 Gy. In the cytotoxicity assays, the concentration of the non-observed effect (NOEC) for irradiated and non-irradiated organisms 0.0001 mg L-1 and observed effect concentration (LOEC) at concentrations above this. The IC25 (72h) for non-irradiated organisms was 0.0003 mg L-1 and IC50 (72h) was 0.0008 mg L -1 for irradiated and non-irradiated organisms. Despite of the concentrations of effect found in this study were higher than in the environment, both measurements are in the same order of magnitude and should be also take into account the possible synergistic effects of DEET with other contaminants in the aquatic environment. (author)

  3. Effect of {sup 60} Co gamma radiation on trehalose level of Saccharomyces boulardii cells; Efeito da radiacao gama {sup 60} Co nos niveis de trealose de celulas de Saccharomyces boulardii

    Energy Technology Data Exchange (ETDEWEB)

    Santos, Raquel Gouvea dos; Neves, Maria Jose [Centro de Desenvolvimento da Tecnologia Nuclear (CDTN), Belo Horizonte, MG (Brazil). Nucleo de Radiobiologia; Duarte, Rinaldo; Nicoli, Jacques R. [Minas Gerais Univ., Belo Horizonte, MG (Brazil). Dept. de Microbiologia

    1996-07-01

    The medicine Floratil used for diarrhea treatment is composed by lyophilized yeast cells of Saccharomyces boulardii. The action mechanism of this product is unknown. The efficacy of S. boulardii depends on its viability. The increase in the trehalose level in S. cerevisiae cells submitted to a previous sub-lethal level heat shock results in resistance to a lethal shock. The aim of this work was to study weather the cell trehalose level is involved also in the resistance of gamma irradiation. It was noted that the yeast cells surviving to gamma irradiation had more trehalose level than control cells. Apparently there is a positive correlation between of trehalose level and gamma irradiation resistance of S.boulardii cells. (author)

  4. Inverse dose-rate effect for the induction of 6-thioguanine-resistant mutants in Chinese hamster V79-S cells by 60Co gamma rays

    International Nuclear Information System (INIS)

    Crompton, N.E.; Barth, B.; Kiefer, J.

    1990-01-01

    Chinese hamster V79-S cells capable of growing in suspension culture were exposed to 60Co gamma rays at a high dose rate (84 Gy/h), low dose rates (200, 50, and 39 mGy/h), and a spectrum of very low dose rates (between 29 and 4.5 mGy/h). Following time for appropriate expression the cultures were assayed for the induction of 6-thioguanine-resistant mutants. For a given dose, a decrease in mutation induction occurred as the dose rate was reduced from high dose rates to low dose rates. However, further reduction in dose rate resulted in a reverse dose-rate effect, and an increase in the frequency of mutants was observed. The contribution of background mutation frequency to this reverse dose-rate effect was studied, both by examining fluctuations of mutation frequency in nonirradiated culture and by its impact upon the dose-rate-independent nature of the reversed effect, and it was found to be negligible. The physiological state of the suspension culture under periods of protracted exposure to very low dose rates was also investigated. The effect of doubling time, plating efficiency, cell cycle distribution, and sensitivity on survival and mutation were examined. In no case was a change apparent during the very low-dose-rate exposures. The results are discussed in terms of the possible expression of cryptic radiation damage after prolonged culture times and/or the involvement of an error-free repair system which requires a certain amount of radiation damage to become active

  5. Soil compaction and gamma radiation ({sup 60}Co) in the development of the cowpea beans [Vigna unguiculata, (L) Walp]; Compactacao do solo e radiacao gama ({sup 60}Co) no desenvolvimento do feijao caupi [Vigna unguiculata, (L) Walp

    Energy Technology Data Exchange (ETDEWEB)

    Viana, Eliane Ferreira; Colaco, Waldeciro [Universidade Federal de Pernambuco (UFPE), Recife, PE (Brazil). Dept. de Energia Nuclear. Radioagronomia]. E-mails: aroucha@ufpe.br; wcolaco@ufpe.br

    2005-08-15

    The objective is to investigate the effect of compaction and increased doses of gamma radiation on the development of cowpea [Vigna unguiculata, (L) Walp] var. IPA-206 cultivated in a Neossolo fluvic soil, artificially compacted. Significant differences were observed in plant height, with the increase doses in soil density (1.30 Mg.m{sup -3} - compacted soil and 1.70 Mg.m{sup -3} - non-compacted soil), and in response to increased doses of irradiation (y-rays) [ [0, 100, 200 and 300 Gy). The diameter of the stem was significantly reduced in response to the increase in soil density, however the same did not occur in relation to the doses of irradiation (y-rays) holding capacity of the soil was reduced in response to the increase in soil density. (author)

  6. Use of 60Co panoramic source in the induction of oral mucositis in rats

    International Nuclear Information System (INIS)

    Andrade, Maira F.; Benetti, Carolina; Zezell, Denise M.; Correa, Luciana

    2013-01-01

    Oral Mucositis is a well-known side effect of chemo-radiotherapy in cancer patients or transplant recipients that could induce hospitalization or impairs therapy in different levels of severity. This study is devoted to define the first steps in the research of low level laser treatments in oral mucositis, proposing a 60 Co radiation to experimentally induce oral mucositis in rats using Panoramic gamma irradiator, simulating usual radiotherapy of head and neck cancer. Fifteen male Wistar rats, above 250g, were irradiated at Centro de Tecnologia das Radiacoes (IPEN - CNEN/SP) and divided in three experimental groups, with different single doses of radiation (30 Gy, 25 Gy and 20 Gy). The animals were observed for a 20 days period. Animals that received 30 Gy and 25 Gy developed greater severity of mucositis and premature euthanasia was performed in these groups on the 7th and 11th day after the irradiation, respectively. The 20 Gy group developed oral mucositis grading from moderated to severe between the days 7 and 11 after irradiation, with progressive body mass loss and decrease in the intake of food and water. These animals recovered from oral mucositis around the 18th day and clinical remission at the 20th day. The single dose of 20 Gy Gamma radiation proved to be efficient way for inducing oral mucositis in rats, allowing the establishment of an experimental model for oral mucositis in rats for future use on interventions of this serious aspect of radiation therapy, such as laser therapy using different wave lengths and power densities. (author)

  7. Use of {sup 60}Co panoramic source in the induction of oral mucositis in rats

    Energy Technology Data Exchange (ETDEWEB)

    Andrade, Maira F.; Benetti, Carolina; Zezell, Denise M., E-mail: mairandrade@yahoo.com, E-mail: zezell@usp.br [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil); Correa, Luciana, E-mail: lcorrea@usp.br [Universidade de Sao Paulo (FO/USP), Sao Paulo, SP (Brazil). Fac. de Odontologia

    2013-07-01

    Oral Mucositis is a well-known side effect of chemo-radiotherapy in cancer patients or transplant recipients that could induce hospitalization or impairs therapy in different levels of severity. This study is devoted to define the first steps in the research of low level laser treatments in oral mucositis, proposing a {sup 60}Co radiation to experimentally induce oral mucositis in rats using Panoramic gamma irradiator, simulating usual radiotherapy of head and neck cancer. Fifteen male Wistar rats, above 250g, were irradiated at Centro de Tecnologia das Radiacoes (IPEN - CNEN/SP) and divided in three experimental groups, with different single doses of radiation (30 Gy, 25 Gy and 20 Gy). The animals were observed for a 20 days period. Animals that received 30 Gy and 25 Gy developed greater severity of mucositis and premature euthanasia was performed in these groups on the 7th and 11th day after the irradiation, respectively. The 20 Gy group developed oral mucositis grading from moderated to severe between the days 7 and 11 after irradiation, with progressive body mass loss and decrease in the intake of food and water. These animals recovered from oral mucositis around the 18th day and clinical remission at the 20th day. The single dose of 20 Gy Gamma radiation proved to be efficient way for inducing oral mucositis in rats, allowing the establishment of an experimental model for oral mucositis in rats for future use on interventions of this serious aspect of radiation therapy, such as laser therapy using different wave lengths and power densities. (author)

  8. Study on the resistance of haloferax radiotolerans, an extreme Halophilic archaebacterium from Uromia lake against ultraviolet (UV) light and 60Co gamma-rays

    International Nuclear Information System (INIS)

    Asgarni, E.; Shirzad, M.; Soudi, M. R.; Shahmohammadi, H. R.; Falsafi, T.

    2006-01-01

    In this work, the capacity of an extreme halophilic archaebacterium, isolated from Uromia lake, Haloferax radiotolerans to withstand the lethal effects of ultraviolet light (UV),and 60 Co r-rays has been studied. The resistibility of this organism against the DNA-damaging agents was evaluated by calculating of the survival fractions at different dose rates of W and 60 Co r-rays radiations and compared with those of Escherichia coli B/r (a radioresistant strain of E. coli). D 37 values for Haloferax radiotolerans and E. coli B/r were 23 1, and 9 J/m 2 , respectively, by exposure to the UV light. They were 645, and 99 Gy, respectively, by exposure to 60 Co r-rays. Against these agents, Haloferax radiotolerans shows much more resistance compare to that of E. coli B/r. This is categorized as the first report of resistibility in the member of Archaea

  9. Effect of gamma radiation of 60Co in the conservation of seeds and on the productivity of bean plant (Phaseolus vulgaris L.)

    International Nuclear Information System (INIS)

    Marcos Filho, Julio

    1971-01-01

    Seeds of the field bean variety 'Goiano Precoce' (Phaseolus vulgaris L.) subjected to various radiation doses ( 60 Co) ( Co) were used in a series of experiments with the objective of studying the different aspects of seed behavior thus treated. The radiation doses, comprising six treatments, varied from 0,0 to 6,4 krad of gamma radiation. Effect on seed germination and seedling dry weight was studied by means of a factorial experiment conducted under laboratory controlled conditions. The factors used were the radiation doses and nine increasing lengths of time from date of seed irradiation. Seed vigor was determined by the rate of seedling emergence when planted in small field plots. A factorial design was used. The variables were the radiation dosages and six lengths of time elapsed since date of seed irradiation. The effect of seed irradiation on yield was evaluated by means of two randomized block design field experiments. After the seed vigor experiment was conducted infestation by the bean weevil, Acanthoscelidcs obtectus Say , was observed in irradiated seeds stored under normal conditions, indicating a relationship between radiation dosage and insect damage. An analysis was made of this effect at fourteen increasing time intervals. The analysis was made according to a factorial scheme considering as factors radiation dosage and time interval. The following conclusions could be drawn from the analysis and discussion of the results obtained: a) Seed germination was adversely affected by all radiation doses in relation to the check treatment. This effect however decreased significantly with storing time. b) Seed vigor was higher for those treated with 0,4 , 0,8 and 1,6 krad when compared with those that were not irradiated. c) Pod and seed weight were lowered by the 1,6 and 6,4 krad radiation doses in relation to the check treatment. d) Infestation by the bean weevil was significantly checked by all radiation treatments in relation to the check treatment. e

  10. Thermal stability of formulations of PVC irradiated with {gamma} of {sup 60}; Estabilidad termica de formulaciones de PVC irradiadas con {gamma} de {sup 60} Co

    Energy Technology Data Exchange (ETDEWEB)

    Martinez P, M.E.; Carrasco A, H. [ININ, A.P. 18-1027, 11801 Mexico D.F. (Mexico)]. E-mail: memp@nuclear.inin.mx; Castaneda F, A.; Benavides C, R.; Garcia R, S.P. [CIQA, 25100 Saltillo, Coahuila (Mexico)

    2004-07-01

    The industry of cables and wires frequently use cable isolations with base of formulations of PVC, in those that stabilizer has usually been used with the help of heavy metals, as the lead, which is toxic. To solve the problem, from the 2002 one has come studying in combined form in the National Institute of Nuclear Research ININ and the Center of Investigation in Applied Chemistry CIQA, the modifications induced by the radiation in formulations with the help of vinyl poly chloride PVC. In these formulations, prepared with cross linking agent, plastifying industrial grade, stuff and non toxic stabilizers of calcium estearate and zinc industrial grade, it is sought to replace the stabilizer of Pb. For this were irradiated it test tubes of PVC with gamma radiation of cobalt 60 to three different dose in atmospheres of air and argon. Later it was determined their thermal stability at different times of heating and it was measured the Young modulus by means of thermo mechanical analysis. Those results obtained together with other techniques of characterization suggest that the irradiated proposed formulation can substitute the one stabilized with lead. (Author)

  11. Comparative effects of 60Co gamma-rays and neon and helium ions on cycle duration and division probability of EMT 6 cells. A time-lapse cinematography study.

    Science.gov (United States)

    Collyn-d'Hooghe, M; Hemon, D; Gilet, R; Curtis, S B; Valleron, A J; Malaise, E P

    1981-03-01

    Exponentially growing cultures of EMT 6 cells were irradiated in vitro with neon ions, helium ions or 60Co gamma-rays. Time-lapse cinematography allowed the determination, for individual cells, of cycle duration, success of the mitotic division and the age of the cell at the moment of irradiation. Irradiation induced a significant mitotic delay increasing proportionally with the delivered dose. Using mitotic delay as an endpoint, the r.b.e. for neon ions with respect to 60Co gamma-rays was 3.3 +/- 0.2 while for helium ions it was 1.2 +/- 0.1. Mitotic delay was greatest in those cells that had progressed furthest in their cycle at the time of irradiation. No significant mitotic delay was observed in the post-irradiation generation. Division probability was significantly reduced by irradiation both in the irradiated and in the post-irradiated generation. The reduction in division probability obtained with 3 Gy of neon ions was similar to that obtained after irradiation with 6 Gy of helium ions or 60Co gamma-rays.

  12. Comparisons of the standards for air kerma of the PTB and the BIPM for 60Co and 137Cs gamma radiation

    International Nuclear Information System (INIS)

    Allisy-Roberts, P.J.; Burns, D.T.; Buermann, L.; Kramer, H.M.

    2005-11-01

    Direct comparisons of the standards for air kerma of the Physikalisch-Technische Bundesanstalt (PTB, Germany) and of the Bureau International des Poids et Mesures (BIPM) were carried out in the 60 Co and 137 Cs radiation beams of the BIPM in 2000. The results, expressed as ratios of the PTB and the BIPM standards for air kerma, indicate a relative difference in 60 Co of 9.9 x 10 -3 with a combined standard uncertainty of 1.8 x 10 -3 , and in 137 Cs of 6.4 x 10 -3 with a combined standard uncertainty of 2.8 x 10 -3 . The earlier comparisons in 60 Co γ rays made in 1971 (direct) and 1989 (indirect) resulted in an agreement of the two standards within 2 x 10 -3 . The differences obtained now are due to the application of new correction factors for wall effects and point source non-uniformity of the beam, k wall and k pn , for the PTB standards, which were calculated using Monte Carlo methods. (authors)

  13. Experimental calibration and determination of the relative response for Lif: Mg, Ti(TLD-100) dosemeters at 60Co gamma and 60 kVp X-ray energies

    International Nuclear Information System (INIS)

    Marco, M.L.; Gonzalez, L.; Delgado, V.; Vano, E.; Moran, P.

    1985-01-01

    The thermoluminescence efficiency of LiF: Mg, Ti (TLD-100) dosemeters has been determined for photon beams from 60 Co gamma rays and 60 kVp X-rays. It has been proven that light yield varies as a function of the photon energy. An experiment was performed using an X-ray beam whose spectrum has been determined by an X-ray fluorescence method. This enabled a direct calculation of the absorbed doses in the T1 material for the different operation conditions. These values and the experimental ones from measuring T1 intensities have been used to obtain the efficiency for energy X-ray spectrum. From the above values, the dosemeter T1 response, relative to 60 Co, has been evaluated. (author)

  14. Relative biological effectiveness measurements using murine lethality and survival of intestinal and hematopoietic stem cells after Fermilab neutrons compared to JANUS reactor neutrons and 60Co gamma rays

    International Nuclear Information System (INIS)

    Hanson, W.R.; Crouse, D.A.; Fry, R.J.M.; Ainsworth, E.J.

    1984-01-01

    The relative biological effectiveness (RBE) of the 25-MeV (average energy) neutron beam at the Fermi National Accelerator Laboratory was measured using murine bone marrow (LD/sub 50/30/) and gut (LD/sub 50/6/) lethality and killing of hematopoietic colony forming units (CFU-S) or intestinal clonogenic cells (ICC). The LD/sub 50/30/ and LD/sub 50/6/ for mice exposed to the Fermilab neutron beam were 6.6 and 8.7 Gy, respectively, intermediate between those of JANUS neutrons and 60 Co γ rays. The D 0 values for CFU-S and ICC were 47 cGy and 1.05 Gy, respectively, also intermediate between the lowest values found for JANUS neutrons and the highest values found after 60 Co γ rays. The split-dose survival ratios for CFU-S at intervals of 1-6 hr between doses were essentially 1.0 for both neutron sources. The 3-hr split-dose survival ratios for ICC were 1.0 for JANUS neutrons, 1.85 for Fermilab neutrons, and 6.5 for 60 Co γ rays. The RBE estimates for LD/sub 50/30/ were 1.5 and 2.3 for Fermilab and JANUS neutrons, respectively. Based on LD/sub 50/6/, the RBEs were 1.9 (Fermilab) and 3.0 (JANUS). The RBEs for CFU-S D 0 were 1.4 (Fermilab) and 1.9 (JANUS) and for jejunal microcolony D 0 1.4 (Fermilab) and 2.8 (JANUS)

  15. DNA double-strand break and apoptosis induction in human lymphocytes in different cycle cell phases by 60Co gamma rays and Bragg peak protons of a medical beam

    International Nuclear Information System (INIS)

    Khachenkova, A.A.; Boreyko, A.V.; Mozhaeva, A.V.; Chausov, V.N.; Ravnachka, I.I.; Amov, I.; Tiunchik, S.I.

    2009-01-01

    A comparative analysis is made of the regularities in the formation of DNA double-strand break and apoptosis induction in peripheral human blood lymphocytes in different cell cycle phases after 60 Co gamma and extended Bragg peak proton irradiation. It is shown that the formation of apoptotic cells in a lymphocyte population increases linearly in all the cell cycle stages after proton irradiation. The maximal DNA double-strand break and apoptosis yield in lymphocytes is observed in the S phase of the cell cycle

  16. Characterization of barite and crystal glass as attenuators in X-ray and gamma radiation shieldings; Caracterizacao da barita e do vidro cristal como atenuadores na blindagem das radiacoes X e gama

    Energy Technology Data Exchange (ETDEWEB)

    Almeida Junior, Airton Tavares de

    2005-03-15

    Aiming to determine the barium sulphate (BaSO{sub 4}) ore and crystal glass attenuation features, both utilized as shieldings against ionizing X and gamma radiations in radiographic installations, a study of attenuation using barite plaster and barite concrete was carried out, which are used, respectively, on wall coverings and in block buildings. The crystal glass is utilized in screens and in windows. To do so, ten plates of barite plaster and three of barite concrete with 900 cm{sup 2} and with an average thickness ranging from 1 to 5 cm, and three plates of crystal glass with 323 cm{sup 2} and with thicknesses of 1, 2 and 4 cm were analyzed. The samples were irradiated with X-rays with potentials of 60, 80, 110 and 150 kilovolts, and also with {sup 60}Co gamma rays. Curves of attenuation were obtained for barite plaster and barite concrete (mGy/mA.min) and (mGy/h), both at 1 meter, as a function of thickness and curve of transmission through barite plaster and barite concrete as a function of the thickness. The equivalent thicknesses of half and tenth value layers for barite plaster, barite concrete and crystal glass for all X-Ray energies were also determined. (author)

  17. Gamma ({sup 60}CO) radiation effects on arcelin protein and evaluation of bean lineages against Acanthoscelides obtectus (Say) and Zabrotes subfasciatus (Boh.) (Col.: Bruchidae);Efeito da radiacao gama ({sup 60}CO) sobre a proteina arcelina e avaliacao de linhagens de feijoeiro a Acanthoscelides obtectus (Say) and Zabrotes subfasciatus (Boh.) (Col.: Bruchidae)

    Energy Technology Data Exchange (ETDEWEB)

    Botelho, Ana Claudia Girardo

    2006-07-01

    The resistance of arcelin carrying seeds of bean lineages (Phaseolus vulgaris L.) against the bean weevils Acanthoscelides obtectus (Say, 1831) and Zabrotes subfasciatus (Bohemann, 1833) (Coleoptera: Bruchidae), and the influence of gamma radiation ({sup 60}CO) on the manifestation of arcelin resistance to Z. subfasciatus were verified. Laboratorial tests, in choice and non-choice tests, with wild specimens carrying Arc-1, Arc-2, Arc-3, Arc-4, Raz-56 and Raz-59 (with Arc-5 alleles) and commercial lineages as control IAC - Carioca and IAC - Arua were conducted. Statistical design was completely randomized, with five repetitions, with 10 g of grains from each lineage samples by portion. Attractiveness, oviposition, emergence, mortality, adults' weigh and longevity, developing period, sexual rate, seeds' weigh loss, infestation and fecundity (Z. subfasciatus) were observed. Gamma radiation doses irradiations, in general, haven't affected the resistance manifestation of lineages carrying arcelin protein variants against the Z. subfasciatus bean weevil, thus, joint application use of both control methods can be recommended. Raz-56 lineage showed high resistance of the antibiosis types and non-preference for oviposition and feeding to Z. subfasciatus, while Raz-59 showed antibiosis and non-preference for feeding, and both (Raz-56 and Raz-59) showed intermediate resistance to A. obtectus, against which lineage Arc-2 was the most harmful to its development, expressing non-preference to feeding and/or antibiosis. (author)

  18. "6"0Co-generated gamma radiation induced fluctuations on in vitro seed germination and callogenesis in Velvet bean (Mucunapruriens L.) seeds

    International Nuclear Information System (INIS)

    Misra, Pragati; Gupta, Ankit; Shukla, Pradeep Kumar

    2017-01-01

    Mucunapruriens exhibit many medicinal properties and almost every part of the plant contains metabolite of medicinal value. Irradiation treatments performed at in vitro culture has been also employed to increase genetic variability and mutants as a potential source of new commercial cultivars. Gamma rays are often used for developing plants varieties that are agriculturally and economically important and have high productivity potential with the minimum input. The callus induction frequency was in declined gradually with the increasing dose of gamma radiation. Gamma treated seeds developed greenish and fragile callus and also showed decreased weight as compare to control which was white greenish, compact and heavier. (author)

  19. Key comparison BIPM.RI(I)-K1 of the air-kerma standards of the MKEH, Hungary and the BIPM in 60Co gamma radiation

    Science.gov (United States)

    Kessler, C.; Burns, D.; Machula, G.

    2018-01-01

    A comparison of the standards for air kerma of the Hungarian Trade Licensing Office (MKEH), Hungary and of the Bureau International des Poids et Mesures (BIPM) was carried out in the 60Co radiation beam of the BIPM in March 2016. The comparison result, evaluated as a ratio of the MKEH and the BIPM standards for air kerma, is 1.0047 with a combined standard uncertainty of 1.9 × 10-3. The results for an indirect comparison made at the same time are consistent with the direct results at the level of 2.6 parts in 103. The results are analysed and presented in terms of degrees of equivalence, suitable for entry in the BIPM key comparison database. Main text To reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database kcdb.bipm.org/. The final report has been peer-reviewed and approved for publication by the CCRI, according to the provisions of the CIPM Mutual Recognition Arrangement (CIPM MRA).

  20. Shelf life of loose flowers of rose cv. Gruss an Teplitz as influenced by 60Co-gamma irradiation followed by dry cool storage

    International Nuclear Information System (INIS)

    Vinod Kumar; Bhattacharjee, S.K.; Sainudhin, Sumy; Chatterjee, S.R.

    2003-01-01

    The two different experiments were carried out firstly on effect of different doses of gamma irradiation on rose loose flowers and secondly on effect of two selected doses of gamma irradiation and dry cool storage for different durations. Different doses of gamma irradiation viz.. 2.5, 5, 10 and 20 krad were employed to freshly harvested half opened flower buds of rose cv. Gruss an Teplitz. It has been recorded that gamma irradiation doses. viz., 2.5 and 5 krad were found suitable in enhancing shelf life of rose loose flowers. Among these two doses, 2.5 krad was found better where 100, 75 and 15 per cent flowers remained fresh after 24, 48 and 72 hours respectively. In rose cv. Gruss an Teplitz loose flowers after treatment with 2.5 krad and storing the same for 1 day at 4 deg C it resulted in 100, 95, 75, 40 and 10 per cent in flowers in fresh state after 24, 48, 72, 96 and 120 hours, respectively, by keeping the flowers at ambient conditions. In control (fresh flowers) all flowers were found wilted up to 96 hours when kept at ambient conditions. It is concluded that rose cv. Gruss an Teplitz loose flowers after treatment with gamma irradiation of 2.5 krad can be further dry cool stored up to 120 hours at 4 deg C without affecting their longevity. (author)

  1. Effect of dose fractionation of 60Co gamma radiation on longevity and reproduction of Sitophilus granarius (L., 1785) (Col., Curculionidae) in wheat

    International Nuclear Information System (INIS)

    Arthur, V.; Walder, J.M.M.; Wiendl, F.M.; Domarco, R.E.; Haddad, S.S.

    1987-01-01

    The effects of fractionated doses of gamma radiation in different interval of time on longevity and reproductivity of Sitophilus granarius (L., 1785) are studied. Gamma radiation was provided at dose rate of 1.56 kGy/hour by a Cobalt 60 source (Gammabeam-650). The insects were divided in two sets: one was irradiated at acute doses and the other in fractionated doses, with interval of 48 hours. Both sets received doses of 0,40, 50, 60, 70, 80, 90 and 100Gy. Mortality and and emergency were determined weekly. (M.A.C.) [pt

  2. Determination of the Kwall correction factor for a cylindrical ionization chamber to measure air-kerma in 60Co gamma beams

    International Nuclear Information System (INIS)

    Laitano, R.F.; Toni, M.P.; Pimpinella, M.; Bovi, M.

    2002-01-01

    The factor K wall to correct for photon attenuation and scatter in the wall of ionization chambers for 60 Co air-kerma measurement has been traditionally determined by a procedure based on a linear extrapolation of the chamber current to zero wall thickness. Monte Carlo calculations by Rogers and Bielajew (1990 Phys. Med. Biol. 35 1065-78) provided evidence, mostly for chambers of cylindrical and spherical geometry, of appreciable deviations between the calculated values of K wall and those obtained by the traditional extrapolation procedure. In the present work an experimental method other than the traditional extrapolation procedure was used to determine the K wall factor. In this method the dependence of the ionization current in a cylindrical chamber was analysed as a function of an effective wall thickness in place of the physical (radial) wall thickness traditionally considered in this type of measurement. To this end the chamber wall was ideally divided into distinct regions and for each region an effective thickness to which the chamber current correlates was determined. A Monte Carlo calculation of attenuation and scatter effects in the different regions of the chamber wall was also made to compare calculation to measurement results. The K wall values experimentally determined in this work agree within 0.2% with the Monte Carlo calculation. The agreement between these independent methods and the appreciable deviation (up to about 1%) between the results of both these methods and those obtained by the traditional extrapolation procedure support the conclusion that the two independent methods providing comparable results are correct and the traditional extrapolation procedure is likely to be wrong. The numerical results of the present study refer to a cylindrical cavity chamber like that adopted as the Italian national air-kerma standard at INMRI-ENEA (Italy). The method used in this study applies, however, to any other chamber of the same type. (author)

  3. Effects of 60Co gamma-ray local irradiation on rat liver on alkaline phosphatase, lactate dehydrogenase and catalase in the liver and serum

    International Nuclear Information System (INIS)

    Hishikawa-Itoh, Youko; Ayakawa, Yoshio; Miyata, Nobuki

    1980-01-01

    Rats were given a single exposure of various doses (0, 5, 50, 500, and 5000 rads) to local irradiation of 60 Co γ-ray on liver. Activities of alkaline phosphatase (ALP), lactate dehydrogenase (LDH) and catalase in the serum and liver were measured at various time intervals after irradiation. These results were summarized as follows; 1. ALP activity in the serum had no effect on irradiation up to 500 rads, but in the case of 5000 rads irradiation exhibited a marked loss from 4 days after irradiation. ALP activity in the liver to 5000 rads exposure on 7 days after irradiation increased, on the other hand in the serum decreased, and the patterns of ALP activities in the liver and serum to the irradiation doses were opposite. 2. LDH activity in the serum by exposure to 5, 500 and 5000 rads increased at 4 days after irradiation, but at 7 days significantly decreased. LDH activity in the liver to the irradiation doses on 7 days after irradiation did not markedly change, but in the serum it tended to be low in inverse proportion to the irradiation doses. 3. Catalase activity in the serum to 50 and 500 rads exposure increased at 4 days after irradiation and decreased at 7 days, but to 5000 rads exposure it decreased in the course of time. Catalase activity in the liver and serum on 7 days after irradiation were inversely proportional to irradiation doses. It is difficult that catalase activity makes a index of clinical irradiation effects, because catalase activity decrease under the various conditions, such as cancer, anemia, infection of bacterias and so on. Since activities of ALP and LDH increase in almost disease, decrease of ALP activity and decrease following temporary increase of LDH activity by irradiation may be able to become a clinical indicator on irradiation effects. (author)

  4. DOMI: mutant of tomato of double purpose obtained by means of the induction of mutations with rays gamma of 60Co

    International Nuclear Information System (INIS)

    Gonzalez, Maria C; Mukandama, JP; Ali, MM; Trujillo Delfina

    2010-01-01

    DOMI is a tomato mutant obtained at the National Institute of Agricultural Sciences (INCA) after irradiating seeds from Amalia variety with 500 Gy gamma rays. This variety has a high productive potential and an appropriate quality for fresh consumption and industrial purposes

  5. Activity evaluation from different native or irradiated with {sup 60} Co gamma rays snake venoms and their inhibitory effect on Leishmania (Leishmania) amazonensis; Avaliacao da atividade de diferentes venenos de serpentes, nativos ou irradiados, com radiacao gama de {sup 60} Co, quanto ao poder inibitorio do crescimento de Leishmania (Leishmania) amazonensis

    Energy Technology Data Exchange (ETDEWEB)

    Lourenco, Cecilia de Oliveira

    2000-07-01

    Cutaneous leishmaniasis is a disease, caused by Leishmania parasites, that occurs frequently in tropical and sub-tropical regions of the world. Skin lesions that could results in disfiguring aspect characterize it. The treatment is based on few drugs as antimony salts or pentamidine that are toxic with increasing resistance by the parasite. Alternative forms of disease treatment are in constant search, including natural components as snake venoms. Previous studies demonstrate that some components of snake venoms have an inhibitory effect against those parasites, including Leishmania species. Although snake venoms presented high toxicity, several methods have been described to detoxify most or some of their toxic components, with favorable results by the use of gamma irradiation. In this report we tested several native and irradiated snake venoms for inhibitory effect against Leishmania (Leishmania) amazonensis parasite and LLCMK{sub 2} mammalian cells, with enzymatic tests and electrophoresis. There are significant activity in Acanthophis antarcticus, Agkistrodon bilineatus, Bothrops moojeni, Bothrops jararaca, Hoplocephalus stephensi, Naja melanoleuca, Naja mossambica, Pseudechis australis, Pseudechis colletti, Pseudechis guttatus and Pseudechis porphyriacus, venom being inactive Pseudonaja textilis, Notechis ater niger, Notechis scutatus. Oxyuranus microlepidotus and Oxyuranus scutellatus venoms. After 2 KGy of {sup 60}Co irradiation most venom loses significantly their activity. Venoms with antileishmanial activity presented L-amino acid oxidase (L-AO) activity and showed common protein with a molecular weight about 60kDa in SDS-PAGE. These results indicate that L-AO activity in those venoms are probably related with antileishmanial effect. (author)

  6. Effects of gamma irradiation of the {sup 60} Co on antimicrobial action of plant extracts of bark and leaves of Schinus terebinthifolius Raddi; Efeitos da radiacao gama do {sup 60} Co sobre a acao antimicrobiana de extratos vegetais de cascas e folhas de Schinus terebinthifolius Raddi

    Energy Technology Data Exchange (ETDEWEB)

    Silva, Edvane Borges da; Santos, Gustavo Henrique Farias dos; Amaral, Ademir de Jesus [Universidade Federal da Pernambuco (GERAR/DEN/UFPE), Recife, PE (Brazil). Dept. de Energia Nuclear. Grupo de Estudos em Radioprotecao e Radioecologia; Lima, Jeniffer Maiza de Souza [Universidade Federal da Pernambuco (CAV/UFPE), Vitoria de Santo Antao, PE (Brazil); Xisto, Kesia; Araujo, Rosilma de Oliveira [Universidade Federal da Pernambuco (DA/UFPE), Recife, PE (Brazil). Dept. de Antibioticos

    2014-07-01

    This study aimed to evaluate the potential for antimicrobial activity in vitro of extracts of bark and leaves of S. terebinthifolius treated with {sup 60}Co gamma radiation. 5,0 doses were used; 7.5 and 10 kGy, being held non-irradiated controls. To determine the antimicrobial activity was applied to the disc diffusion technique to evaluate the diameter of the inhibition zones against Gram-positive bacteria, gram-negative bacteria, alcohol-acid-resistant and yeast. Antimicrobial activity was considered significant for halos ≥ 15 mm. The results indicate an intensification of antimicrobial action of bark extracts, the 5.0 kGy, against S. aureus. Was held the micro dilution in broth to determine the minimum inhibitory concentration (MIC) and Minimum Bactericidal Concentration (MBC) of peels extracts, compared to eight clinical isolates of S. aureus. The MBC values showed that ionizing radiation did not produce the increased of anti bacteriostatic action of S. terebinthifolius, but the results indicated that S. terebinthifolius bark extracts can be used as an antimicrobial agent and ionizing radiation as an important alternative in this conservation feature.

  7. Seeds of Camargue rice (Oryza Sativa L. Var Cigalon) and their responses to exposure to 60-Co gamma radiations. Study of the natural phenomenon of radiation protection

    International Nuclear Information System (INIS)

    Bayonove-Barthelemy, Jacqueline

    1978-01-01

    Within the frame of a project aiming at improving a technique of experimental mutagenesis (seed processing by cobalt-60 gamma rays), this research thesis reports the radiobiological characterization of Cigalon rice seeds, the adjustment of techniques of radiation protection, and the definition of processing and irradiation conditions which could reduce the undesirable physiological effects of cobalt-60 gamma radiation on rice seeds. The author reports the study of variations of physiological radio-sensitivity. Damages are assessed by comparing the growth of irradiated seeds with that of non-irradiated seeds. The mechanism of radiation natural protection is studied by comparing an inhibition in distilled water at two temperatures (5 and 24 C) with that obtained with cysteamine (an efficient radiation protection compound)

  8. Cytotoxicity evaluation of Diethyltoluamide (DEET) in Perna perna (Linnaeus, 1758) mussels non-irradiated and irradiated with {sup 60}Co gamma radiation; Avaliacao da citotoxicidade do dietiltoluamida (DEET) em mexilhoes Perna perna (Linnaeus, 1758) irradiados e nao irradiados com radiacao gama de {sup 60}Co

    Energy Technology Data Exchange (ETDEWEB)

    Martini, Gisela de Assis

    2013-07-01

    Recent studies have identified the presence of several emerging pollutants in aquatic environments. The occurrence in different environmental matrices has been continuously reported, highlighting the need for toxicity studies. The DEET (N,N-diethyl-meta-toluamide) is the active ingredient used in most insect repellents, and is present in many commercially available formulations. Apart from chemical pollutants, aquatic organisms are subject to exposure of ionizing radiation from natural sources or in the vicinity of nuclear power plants. The present study evaluated the toxicity of DEET in organisms irradiated and non-irradiated with {sup 60}Co gamma radiation, and the effects that radiation causes in lysosomes of hemocytes of Perna perna mussel. For this purpose, assays were performed to identify the acute toxicity of DEET concentration and the dose of gamma radiation able to cause mortality. Subsequently, cytotoxicity assays were carried out to assess the stability of the lysosomal membrane in organisms exposed to ionizing radiation and DEET. According to the results obtained in acute toxicity tests, the concentration of DEET that causes mortality of 50% exposed organisms (LC50) is 114,27 mg L{sup -1}, and the radiation dose that causes mortality (LD50) is 1068 Gy. In the cytotoxicity assays, the concentration of the non-observed effect (NOEC) for irradiated and non-irradiated organisms 0.0001 mg L-1 and observed effect concentration (LOEC) at concentrations above this. The IC25 (72h) for non-irradiated organisms was 0.0003 mg L-1 and IC50 (72h) was 0.0008 mg L{sup -1} for irradiated and non-irradiated organisms. Despite of the concentrations of effect found in this study were higher than in the environment, both measurements are in the same order of magnitude and should be also take into account the possible synergistic effects of DEET with other contaminants in the aquatic environment. (author)

  9. Effect of Irradiation of 60CO Gamma Rays on Growth of Garlic (Allium Sativum L) Plants Cv. Lumbu Hijau at Low Land Area

    International Nuclear Information System (INIS)

    Ismiyati Sutarto; Kumala Dewi; Arwin; Nurrohma

    2004-01-01

    Garlic originally come from the sub tropical area. In Indonesia, garlic is grown generally in high land area with an altitude between 1000 - 1600 m above sea level. Therefore, the area for growing and producing garlic is limited. Besides, genetic variation of garlic is very narrow since garlic belongs to vegetatively propagated crops. An effort for increasing genetic variation of garlic was done by exposing garlic cloves to gamma rays in order to obtain garlic mutant lines adapted to low land area. Garlic cloves were exposed to different doses of gamma rays 0 (untreated) 2, 4, 6, 8 and 10 Gy at the Centre for Research and Development of Isotope and Radiation Technology. Each dose consisted of 150 garlic cloves. Untreated and irradiated garlic cloves were grown at Bandar Buat Experimental Station (50 m above sea level), Padang, West Sumatera. The experiment was arranged in a randomized block design with three replication. The parameter observed were percentage of grown plants and survival harvested plants, plant height, leaf number, chlorophyll content, number of stomata, plant age, number of cloves per bulbs, fresh, dry weight and diameter of bulbs. The result indicated that the dose of gamma rays 6 Gy is an advantage dose for obtaining well adapted garlic mutant lines in the low land area. (author)

  10. The combined effect of the gamma radiations of 60 Co and different chemicals on the cells of the radicular vegetative tip of Allium sativum L

    International Nuclear Information System (INIS)

    Dumitrescu, Rodica; Popescu, D.; Andrei, M.

    1999-01-01

    The gamma ionizing radiations used alone in the treatment of Allium Sativum L. affected differently the cells belonging to the two zones of the root causing modifications of the cellular parameters which have been previously estimated. Data in the literature established that the damage of the interphasic nucleus of the cells determines the obstruction of the DNA synthesis and the delay of the cell mitosis. Sparrow, A. H., Miller, M. W. and Al-Rubeai confirmed the existence of a relationship between the nuclear volumes and the radiosensitivity of the different types of cells. The aim of this experiment was to counteract the harmful effect of gamma radiations at cellular level with the help of combined treatments, namely, the gamma radiations associated with different chemicals having radioprotective effects. The biological significance of the obtained values was established by statistical calculations with the help of the Student (t) test. The values of the estimated cellular parameters, NV, ICV, CV, NCR showed that, although there were certain fluctuations, their values were not always significant. (author)

  11. Dose-effect relationships for fife shortening, tumorigenesis, and systemic injuries in mice irradiated with fission neutron or 60Co gamma radiation

    International Nuclear Information System (INIS)

    Ainsworth, E.J.; Fry, R.J.M.; Williamson, F.S.; Brennan, P.C.; Stearner, S.P.; Yang, V.V.; Crouse, D.A.; Rust, J.H.; Borak, T.B.

    1977-01-01

    The objective of this research is to provide additional data on life shortening, neoplastic and non-neoplastic diseases, and other systematic injuries necessary for the determination of dose-response relationships. The data are used to test existing predictive models and formulate new models which may assist with radiation risk assessment. Late somatic effects of fission neutrons from the JANUS reactor or from cobalt-60 gamma radiation are evaluated in young adult B6CF 1 mice that receive either a range of single doses or protracted doses at low dose rates; the protracted irradiation is administered over a 6-month period. After single doses of gamma radiation the relationship between radiation dose and percent life shortening appears linear whereas after single doses of fission spectrum neutrons a non-linear dose response is observed. These results suggest that estimates of radiation risk for fission spectrum neutrons should take into account the following: the curvilinearity of the neutron dose-response curve for life shortening, and the increased life shortening produced by neutron dose fractionation

  12. IV. Effect of 60Co gamma rays on survival rate of China aster plants (Callistephus chinensis Nees in M1 generations - under field conditions

    Directory of Open Access Journals (Sweden)

    A. Wosińska

    2013-12-01

    Full Text Available Studies were undertaken on the effect of different doses of gamma radiation on survival rate of plants (germinated from irradiated seeds for 5 China aster varieties specified at florescence time. During their growth under field conditions (from planting time to blooming lethal effect of the radiation occurred in plants of all varieties and its level depended on dose and variety. Effect of 3 kR and 6 kR doses differed depending on variety and was not always harmful, but following irradiation with doses exceeding 6 kR a considerable decrease in survival rate was observed. Radioresistance of studied varieties - measured both: by LD50 and LDl00 - differed; depending on variety, LD50 and LDl00 values fluctuated: from 6 to 9 kR and 12 to 15 kR respectively.

  13. The influence of 60Co gamma radiation on the action of phenolic compounds Anadenanthera colubrina (Vell.) Brenan in the microbiological control of crude extracts

    International Nuclear Information System (INIS)

    Santos, Gustavo Henrique F. dos; Silva, Edvane B. da; Sena, Kesia Xisto da Fonseca R. de; Silva, Bruna L.; Lima, Claudia S. de A.

    2009-01-01

    Anadenanthera colubrina (Vell.) Brenan, known popularly as angico is a tree founded in northeastern of Brazil, and has great economic and ecological interest. These trees are characterized by high levels of tannins. To evaluate the influence of gamma radiation in plant extracts of barks and leaves of angico, we got extracts of barks and leaves in 70% ethanol, which was filtered and evaporated until dryness. It were classified in four groups (non-irradiated, 5; 7.5 and 10 kGy). The total phenols were quantified by Folin-Ciocalteau method, and the tannins, using the precipitation of casein. The antimicrobial activity of extracts was observed using the paper disk diffusion methods against Gram-positive, Gram-negative, acid-alcohol-resistant bacteria: and yeasts. The halos greater than 10 mm were considered actives. It was also performed the Minimal Inhibitory Concentration (MIC) using Gram positive and alcohol-acid resistant bacteria. The results showed that tannins of A. colubrina, were 52 to 57% of total phenols, and leaves, 58 to 60%. The percentage of total phenols and tannins in the barks and leaves of A. colubrina have not statistically significant change when compared with the irradiated control. These results suggest that A. colubrina has antimicrobial activity against the Gram-positive and alcohol-acid -resistant bacteria, confirmed by MIC. The extracts of leaves it was additionally actives against the Gram-negative and against the yeast C. albicans. These results show that the gamma radiation has not influence about the antimicrobial activity of crude extracts of barks and leaves of A. colubrina. (author)

  14. The influence of {sup 60}Co gamma radiation on the action of phenolic compounds Anadenanthera colubrina (Vell.) Brenan in the microbiological control of crude extracts

    Energy Technology Data Exchange (ETDEWEB)

    Santos, Gustavo Henrique F. dos; Silva, Edvane B. da, E-mail: santosghf@hotmail.co [Universidade Federal de Pernambuco (UFPE), Recife, PE (Brazil). Dept. de Energia Nuclear. Grupo de Estudos em Radioprotecao e Radioecologia (GERAR); Sena, Kesia Xisto da Fonseca R. de; Silva, Bruna L., E-mail: kxfrs@bol.com.b [Universidade Federal de Pernambuco (UFPE), Recife, PE (Brazil). Dept. de Antibioticos; Lima, Claudia S. de A., E-mail: claudia.salima@gmail.co [Universidade Federal de Pernambuco (UFPE), Recife, PE (Brazil). Dept. de Biofisica e Radiobiologia

    2009-07-01

    Anadenanthera colubrina (Vell.) Brenan, known popularly as angico is a tree founded in northeastern of Brazil, and has great economic and ecological interest. These trees are characterized by high levels of tannins. To evaluate the influence of gamma radiation in plant extracts of barks and leaves of angico, we got extracts of barks and leaves in 70% ethanol, which was filtered and evaporated until dryness. It were classified in four groups (non-irradiated, 5; 7.5 and 10 kGy). The total phenols were quantified by Folin-Ciocalteau method, and the tannins, using the precipitation of casein. The antimicrobial activity of extracts was observed using the paper disk diffusion methods against Gram-positive, Gram-negative, acid-alcohol-resistant bacteria: and yeasts. The halos greater than 10 mm were considered actives. It was also performed the Minimal Inhibitory Concentration (MIC) using Gram positive and alcohol-acid resistant bacteria. The results showed that tannins of A. colubrina, were 52 to 57% of total phenols, and leaves, 58 to 60%. The percentage of total phenols and tannins in the barks and leaves of A. colubrina have not statistically significant change when compared with the irradiated control. These results suggest that A. colubrina has antimicrobial activity against the Gram-positive and alcohol-acid -resistant bacteria, confirmed by MIC. The extracts of leaves it was additionally actives against the Gram-negative and against the yeast C. albicans. These results show that the gamma radiation has not influence about the antimicrobial activity of crude extracts of barks and leaves of A. colubrina. (author)

  15. Effect of dose-rate of gamma irradiation (60Co) on the anti nutritional compounds phytic acid and antitrypsin on soybean (glycine max L.)

    International Nuclear Information System (INIS)

    Tanhindarto, R.P.; Hariyadi, P.; Purnomo, E.H.; Irawati, Z.

    2013-01-01

    An investigation on the effect of gamma irradiation at different dose-rate on the anti-nutritional compounds (phytic acid and antitrypsin) and the color of soybean has been conducted. The purpose of the study was to analyze the influence of the dose-rate on the rate of change of anti-nutritional compounds and color. Samples were irradiated with dose-rates of 1.30; 3.17; 5.71 and 8.82 kGy/hour with irradiation time varied from 0.5 to 55 hours. Phytic acid content and antitrypsin activity, as well as their L α b color values were analyzed. Results showed that a simple first order kinetics model can be used to describe changes in the concentration of the anti-nutritional compounds and color soybeans during the radiation processing. Data indicate that irradiation process at higher dose-rate (shorter time) is more effective in destroying anti-nutritional compounds as compared to that of irradiation process at lower dose-rate (longer time). Furthermore, irradiation process at higher dose-rate (shorter time) also have less detrimental effect on color of the soybean and the resulted soybean flour as compared to that of irradiation process at lower dose-rate (longer time). These findings suggest that irradiation process at a same dose may potentially be optimized by selecting the most appropriate combination of dose-rate and time of irradiation. (author)

  16. The influence of 60Co gamma rays to cell reproduction (An experiment using low dose levels on vero and primary monkey kidney cells)

    International Nuclear Information System (INIS)

    Danusupadmo, C.J. Sugiarto

    1985-01-01

    Vero and primary monkey kidney cells in culture were gamma irradiated with doses of 0, 0.4 and 0.8 Gy at a dose-rate of 1.30-1.45x10 3 Gy/hour. At harvest time 3 days post irradiation, 0.4 Gy proved to be able to lower the number of vero cells in such a degree that it became significantly different from the control, whereas 0.8 Gy could not suppress the number of primary cells to a level that differed significantly from its control. At harvest time of 7 days post irradiation, 0.4 Gy was found effective in lowering both vero and primary cells so that the number of the harvested cells were significantly different from the controls. At harvest time of 3 days post irradiation, 0.8 Gy caused both cell types reached levels that were not significantly different from 0.4 Gy, but at 7 days post irradiation the number of vero cells was very significantly different from that of 0.4 Gy, while the number of primary cells remained equal to that of 0.4 Gy. This phenomenon showed that irradiation could cause greater injurious effect at more advanced post irradiation times, while the more proliferative vero cells proved to be more susceptible to irradiation than primary cells, but at the same time more potential in performing repair. (author)

  17. Study of the effect of gamma irradiation (60CO) on the quality of sugar cane spirit and on the cask of maturation

    International Nuclear Information System (INIS)

    Miranda, M.B. de; Horii, J.; Alcarde, A.R.

    2006-01-01

    The ageing or maturing process of drink improves the sensorial characteristics of the product making it a qualified drink with higher economical value. The traditional maturing method of drinks involves their interaction with wood and irradiation can accelerate this ageing process. Sugar cane spirit and oak barrels with a 20 L capacity were treated using gamma irradiation (150 Gy). Physical-chemical and chromatographic analyses were constantly performed for 390 days during the drink ageing period. The sugar cane spirit and barrel irradiation did not change most volatile components of the same type coefficient such as volatile acidity, esters, superior alcohols and furfural throughout the 390 days. There is evidence, however, that some component parameters like aldehydes, tannin, color and copper concentration are in some way influenced resulting in partial acceleration of the ageing or maturing process. At the end of the ageing period, a sensorial analysis was made using 30 non-trained people who tasted the drink. The ageing process acceleration was confirmed by the sensorial evaluation, where the irradiated sugar cane spirit and/or barrel had higher approval regarding all parameters analyzed (scent, flavor and appearance) [pt

  18. Evaluating the effect of gamma radiation (60Co) on protein arcelin and its influence in the resistance of Zabrotes subfasciatus (Boh., 1833) (Coleoptera: Bruchidae)

    International Nuclear Information System (INIS)

    Teixeira, Valeria Wanderley

    1998-01-01

    The objective of this research was to evaluate the effects of different gamma doses of Cobalt-60 on arcelin protein in the manifestation of resistance to Zabrotes subfasciatus (Boh., 1833). Seeds of four lines of Phaseolus vulgaris carriers of arcelin protein (Arcelin-1, Arcelin-2, Arcelin-3 and Arcelin-4) and a cultivar without this protein were used as control (IAC-Carioca Akyta) obtained from the Instituto Agronomico do Estado de Sao Paulo - Nucleo Experimental de Campinas (IAC), were irradiated in a source of Cobalt-60, of the panoramic type, from the Instituto de Pesquisas Energeticas e Nucleares/CNEN/SP. The activity was approximately 2218.79 Ci, and the dose rate 0.678 kGy/h. The doses used were 0; 0.25; 0.5; 1.0 and 2.0 kGy. The results showed that the radiation doses did not influence the parameters evaluated in the resistance because a high degree of antibiose in the Arcelin-1 and Arcelin-2 lines was maintained. The Arcelin-3 and Arcelin-4 lines also maintained their behavior less expressive of resistance by antibiose only prolonging the period from egg to adult. The electrophoretic analysis of the lines and cultivar were not changed in relation to the radiation doses. But there was a decrease in relation to the intensity of color of the bands (absorbance) with the increase of the doses. (author)

  19. Effects of 60Co gamma irradiation on Eimeria falciformis (Eimer 1870) Schneider 1875, a protozoan parasite of the mouse, Mus musculus L

    International Nuclear Information System (INIS)

    Wright, A.C.

    1976-01-01

    Oocysts of an E. falciformis strain first isolated in Wuppertal, Germany were exposed to cobalt-60 gamma radiation under different conditions of oocyst age and development. In order to construct survival curves for oocysts irradiated in the unsporulated state, normalized percent sporulation was plotted as a function of radiation dose. Oocysts irradiated in the sporulated state never caused infection after receiving doses of 50 krads or more. Rarely, oocysts which had received 40 krads produced infection and completed their life cycle in susceptible hosts, but usually the doses beyond which infection did not occur ranged from 30 to 35 krads. Immunity was shown best by hosts which had received unirradiated oocysts in vaccinations prior to challenge. When irradiated sporulated oocysts were used in attempts to immunize susceptible hosts against coccidiosis, the most positive results were obtained from oocysts which had been exposed to 20 krads. Animals receiving vaccinations of oocysts exposed to 20 krads always survived the minimal infections caused by vaccination and also exhibited good immunity to challenge

  20. Study of the effect of gamma irradiation (60 Co) on the quality of sugar cane spirit and on the cask of maturation

    International Nuclear Information System (INIS)

    Miranda, Mariana Branco de; Horii, Jorge; Alcarde, Andre Ricardo

    2006-01-01

    The ageing or maturing process of drink improves the sensorial characteristics of the product making it a qualified drink with higher economical value. The traditional maturing method of drinks involves their interaction with wood and irradiation can accelerate this ageing process. Sugar cane spirit and oak barrels with a 20 L capacity were treated using gamma irradiation (150 Gy). Physical-chemical and chromatographic analyses were constantly performed for 390 days during the drink ageing period. The sugar cane spirit and barrel irradiation did not change most volatile components of the same type coefficient such as volatile acidity, esters, superior alcohols and furfural throughout the 390 days. There is evidence, however, that some component parameters like aldehydes, tannin, color and copper concentration are in some way influenced resulting in partial acceleration of the ageing or maturing process. At the end of the ageing period, a sensorial analysis was made using 30 non-trained people who tasted the drink. The ageing process acceleration was confirmed by the sensorial evaluation, where the irradiated sugar cane spirit and/or barrel had higher approval regarding all parameters analyzed (scent, flavor and appearance). (author)

  1. Development of an analytical model for the determination of 60Co in aqueous samples by atomic absorption and gamma spectrometry techniques

    International Nuclear Information System (INIS)

    Solis A, N. A.

    2015-01-01

    The applications of the nuclear energy in industry and medicine generate radioactive wastes that must be isolated and confined in order to limit its spread in the biosphere. These types of wastes are generated in hospitals, industry, research centers and nuclear power plants (during de fuel cycle). The radioactive elements (radionuclides) cannot be destroyed by any known method, either chemical or mechanical. Its final destruction is produced by radioactive decay, which makes them stable isotopes, or nuclear transmutation being bombarded with atomic particles. Consequently, the radioactive waste management is to control the radioactive discharges and reduce to tolerable limits, eliminating of effluents and wastes the radionuclides of interest, concentrating them so they can be stored or evacuated so that later not appear in dangerous concentration in the biosphere. In Mexico, the main generators of radioactive wastes are the nuclear power plant of Laguna Verde in Veracruz, Hospitals of the public and private sector, in addition Instituto Nacional de Investigaciones Nucleares (ININ) some institutes and facilities of the UNAM and Instituto Politecnico Nacional (IPN) and industries among others. The aqueous radioactive wastes, particularly those from the nuclear power plants contain traces of cobalt-60. Currently there is no model or analytical technique for the separation of this radionuclide, so it became necessary to develop an analytical model for separation and facilitate their disposal. In this paper was shown that atomic absorption and gamma spectrometry techniques can separate the active concentration of the inactive, which is important because the total concentration can be associated through direct relationships with the wear of metal parts of the nuclear reactor, since the metal alloys of the same reactor containing a fraction of cobalt. Also this analytical and mathematical model that can be reproducible and applicable to full sets of samples and that this

  2. Effect of {sup 60}Co gamma radiation on the levels of phenolic compounds from crude extracts of bark of Spondias luta L

    Energy Technology Data Exchange (ETDEWEB)

    Santos, Gustavo H.F.; Silva, Hianna A.M.F.; Melo, Mychely S., E-mail: santosghf@hotmail.com, E-mail: hiannaamfs@gmail.com, E-mail: mychely.melo@gmail.com [Universidade Federal de Pernambuco (UFPE), Recife, PE (Brazil); Silva, Edvane B., E-mail: edvborges@yahoo.com [Universidade Federal de Pernambuco (UFPE), Vitoria de Santo Antao, PE (Brazil). Centro Academinco de Vitoria

    2013-07-01

    Spondias luta L. (Anacardiaceae), popularly known as cajazeira, is a plant widespread in several regions of Brazil, famous for containing phenolic compounds, which are responsible for your characteristic astringent. Ionizing radiations have the ability to cross the material, ionizing atoms and molecules, causing changes in atoms and molecules important. It is known ionizing radiation promotes quantitative and qualitative changes in plant materials, increasing, decreasing or inactivating secondary substances. The objective of this work was to evaluate the effect of gamma radiation as a modifier of the activity of the phenolic compounds of the bark extract of S. luta L. Methods: For the dosage of phenol extracts (control, irradiated with 5 kGy, 10 kGy, 15 kGy and 20 kGy) were diluted in methanol to a final concentration of 200 mg / L. In test tubes were added 50 μL of extract plus 1 ml of distilled water and 500 μl of Folin (diluted 1:10). After 10 minutes in a dark chamber was added 2.5 ml of calcium carbonate to 20% and the content of the tube was homogenized. After 20 minutes was performed with a spectrophotometer at 735 nm. The assay was performed in triplicate and calculated from a standard curve solution of gallic acid and expressed in μEAG (GAE/mg extract). Results: The control extracts, irradiated to 5 kGy, 10 kGy, 15 kGy and 20 kGy, had, respectively, 6.25, 6.70, 6.25, 6.85, 6.45 μEAG/mg of extract. Conclusion: The results showed no significant change in the amount of phenolic compounds, showing that these compounds are radioresistant extract these doses. (author)

  3. Effect of 60Co gamma radiation on the levels of phenolic compounds from crude extracts of bark of Spondias luta L

    International Nuclear Information System (INIS)

    Santos, Gustavo H.F.; Silva, Hianna A.M.F.; Melo, Mychely S.; Silva, Edvane B.

    2013-01-01

    Spondias luta L. (Anacardiaceae), popularly known as cajazeira, is a plant widespread in several regions of Brazil, famous for containing phenolic compounds, which are responsible for your characteristic astringent. Ionizing radiations have the ability to cross the material, ionizing atoms and molecules, causing changes in atoms and molecules important. It is known ionizing radiation promotes quantitative and qualitative changes in plant materials, increasing, decreasing or inactivating secondary substances. The objective of this work was to evaluate the effect of gamma radiation as a modifier of the activity of the phenolic compounds of the bark extract of S. luta L. Methods: For the dosage of phenol extracts (control, irradiated with 5 kGy, 10 kGy, 15 kGy and 20 kGy) were diluted in methanol to a final concentration of 200 mg / L. In test tubes were added 50 μL of extract plus 1 ml of distilled water and 500 μl of Folin (diluted 1:10). After 10 minutes in a dark chamber was added 2.5 ml of calcium carbonate to 20% and the content of the tube was homogenized. After 20 minutes was performed with a spectrophotometer at 735 nm. The assay was performed in triplicate and calculated from a standard curve solution of gallic acid and expressed in μEAG (GAE/mg extract). Results: The control extracts, irradiated to 5 kGy, 10 kGy, 15 kGy and 20 kGy, had, respectively, 6.25, 6.70, 6.25, 6.85, 6.45 μEAG/mg of extract. Conclusion: The results showed no significant change in the amount of phenolic compounds, showing that these compounds are radioresistant extract these doses. (author)

  4. Action of Bothrops moojeni venom and its L-amino acid oxidase fraction, treated with 60Co gamma rays, in Leishmania spp

    International Nuclear Information System (INIS)

    Cardoso, Andre Gustavo Tempone

    1999-01-01

    Bothrops moojeni venom showed an anti leishmania activity in vitro, as determined by a cell viability assay using the reduction of MTT. After venom purification, by chromatography techniques, the fractions with anti leishmania and L-amino acid oxidase activities, eluted in the same positions. The molecular weight of the enzyme was estimated to be 140 kDa by molecular exclusion chromatography, and 69 kDa, by SDS-PAGE, migrating as a single band, with an isoelectric point of 4.8 as determined by isoelectric focusing. The purified LAO from B. moojeni venom, 135-fold more active than crude venom, showed homo dimeric constitution, and was active against Leishmania spp from the New World, with an effective concentration against L(L). amazonensis of 1.80 μg/ml (EC 50 ), L.(V.) panamensis (0.78 |μg/ml) and L.(L.) chagasi (0.63 (μg/ml). Ultrastructural studies of promastigotes affected by LAO demonstrated cell death, with edema in several organelles such as mitochondria and nuclear membrane, before cell disruption and necrosis. The action of LAO was demonstrated to be hydrogen peroxide-dependent. Studies with LLCMK-2 cells, treated with LAO, showed a toxic effect, with an EC 50 of 11|μg/ml. Irradiation of LAO with 6 0C o gamma rays, did not affect its whole oxidative activity, neither detoxified the enzyme. Amastigotes treated with LAO were not affected by its hydrogen peroxide, otherwise, the exogenous product, killed amastigotes with an EC 50 of 0.67mM. These data could be of help in the development of alternative therapeutic approaches to the treatment of leishmaniasis. (author)

  5. The development of 60Co γ knife therapy sources

    International Nuclear Information System (INIS)

    Wu Rihua; Li Xingyi; Wu Guihua; Xia Bing; Zhang Jirong; Chen Tieguang

    2001-01-01

    60 Co γ gamma knife source is a kernel unit of γ knife therapy equipment. It has small active core, high specific activity, narrow half dark area, best focus form, long use period. The research of 60 Co γ knife sources are presented. Specifications of γ knife sources, technical parameters, safety performance, structure feature, procedure and quality control during research are discussed. 60 Co γ source consists of high specific activity cobalt pellets and double stainless steel sealed by argon arc welding. Its safety performance, surface contamination and leakage testing meet the requirements of GB 4075 and GB 4076

  6. Influence Of Dilution Factor For Activity Measurement Of 60CO

    International Nuclear Information System (INIS)

    Hermawan-Candra; Nazaroh; Ermi-Juita

    2003-01-01

    Influence of dilution factor for activity measurement of 60 Co has been studied. The aim of this research is to determine influence between activity measurement result of 60 Co before and after diluted. Measurement were done by using ionization chamber detectors system and gamma spectrometry system with NaI(TI) detector. Discrepancy within three ionization chambers measurements were 0.2% - 2.1% and NaI(Tl) were 3.5% - 6%. (author)

  7. Tenth value layers for 60Co gamma rays and for 4, 6, 10, 15, and 18 MV x rays in concrete for beams of cone angles between 0 degrees and 14 degrees calculated by Monte Carlo simulation.

    Science.gov (United States)

    Jaradat, Adnan K; Biggs, Peter J

    2007-05-01

    The calculation of shielding barrier thicknesses for radiation therapy facilities according to the NCRP formalism is based on the use of broad beams (that is, the maximum possible field sizes). However, in practice, treatment fields used in radiation therapy are, on average, less than half the maximum size. Indeed, many contemporary treatment techniques call for reduced field sizes to reduce co-morbidity and the risk of second cancers. Therefore, published tenth value layers (TVLs) for shielding materials do not apply to these very small fields. There is, hence, a need to determine the TVLs for various beam modalities as a function of field size. The attenuation of (60)Co gamma rays and photons of 4, 6, 10, 15, and 18 MV bremsstrahlung x ray beams by concrete has been studied using the Monte Carlo technique (MCNP version 4C2) for beams of half-opening angles of 0 degrees , 3 degrees , 6 degrees , 9 degrees , 12 degrees , and 14 degrees . The distance between the x-ray source and the distal surface of the shielding wall was fixed at 600 cm, a distance that is typical for modern radiation therapy rooms. The maximum concrete thickness varied between 76.5 cm and 151.5 cm for (60)Co and 18 MV x rays, respectively. Detectors were placed at 630 cm, 700 cm, and 800 cm from the source. TVLs have been determined down to the third TVL. Energy spectra for 4, 6, 10, 15, and 18 MV x rays for 10 x 10 cm(2) and 40 x 40 cm(2) field sizes were used to generate depth dose curves in water that were compared with experimentally measured values.

  8. Efeito da irradiação (60Co na frequência fúngica de amendoim in natura em função do tempo de prateleira Gamma-irradiation effect (60Co in fungi frequency of peanut in natura and after storage

    Directory of Open Access Journals (Sweden)

    Guilherme Prado

    2006-10-01

    Full Text Available Foi verificado o efeito da irradiação gama (60Co na capacidade de destruir a microbiota fúngica, em amendoim em grão, cultivar Tatu Vermelho, da safra 2003 (segundo semestre. Os grãos de amendoim, após a irradiação, foram mantidos à temperatura ambiente em embalagem plástica comercial, durante 180 dias. Para a determinação da porcentagem fúngica foi utilizada a técnica do plaqueamento direto utilizando o meio Ágar Dicloran Rosa de Bengala Cloranfenicol (DRBC, desinfetando ou não os grãos com solução de hipoclorito de sódio. Em grãos de amendoim irradiados e desinfetados externamente, observou-se redução da infecção fúngica a 5 kGy e destruição total de fungos a 10 kGy, após 180 dias de armazenamento à temperatura ambiente. Em grãos irradiados e não desinfetados externamente foram verificados, em função do tempo de armazenamento, aumento da população de fungos com a dose de 1 kGy, redução com a dose de 5 kGy e eliminação total com a aplicação de 10 kGy. A irradiação gama, na dose de 10 kGy ou superior, demonstrou ser um processo eficaz na redução da microbiota fúngica de amendoim em grão, cultivar Tatu Vermelho.Gamma-irradiation effect was verified on the capability of destroying the mycoflora of peanuts grain, Tatu Vermelho cultivar, 2003 crop (second semester. The peanuts grains, after irradiation, were kept at room temperature in plastic bags during 180 days. To determine the percentage of fungi, the direct plating technique was used and the grains were plated out on mycological media dicholoran rose bengal chloranphenicol (DRBC, being desinfected or not with sodium hypochlorite solution. Irradiated and desinfected peanuts was observed a reduction of fungi infection at 5 kGy and total fungi destruction at 10 kGy, after 180 days in storage at room temperature. Irradiated and non desinfected grains showed a increase of fungi population with 1 kGy dose, reduction with 5 kGy dose and total

  9. Dose-dependent and gender-related radiation-induced transcription alterations of Gadd45a and Ier5 in human lymphocytes exposed to gamma ray emitted by 60Co

    International Nuclear Information System (INIS)

    Tavakoli, H.; Manoochehri, M.; Mosalla, S. M. M.; Ghafori, M.; Karimi, A. A.

    2013-01-01

    Growth arrest DNA damage-inducible 45a gene (Gadd45a) and immediate early response gene 5 (Ier5) have been emphasised as ideal radiation bio-markers in several reports. However, some aspects of radiation-induced transcriptional alterations of these genes are unknown. In this study, gender-dependency and dose-dependency as two factors that may affect radiation induced transcription of Gadd45a and Ier5 genes were investigated. Human lymphocyte cells from six healthy voluntary blood donors (three women and three men) were irradiated in vitro with doses of 0.5-4.0 Gy from a 60 Co source and RNA isolated 4 h later using the High Pure RNA Isolation Kit. Dose and gender dependency of radiation-induced transcriptional alterations of Gadd45a and Ier5 genes were studied by quantitative real-time polymerase chain reaction. The results showed that as a whole, Gadd45a and Ier5 gave responses to gamma rays, while the responses were independent of radiation doses. Therefore, regardless of radiation dose, Gadd45a and Ier5 can be considered potential radiation bio-markers. Besides, although radiation-induced transcriptional alterations of Gadd45a in female and male lymphocyte samples were insignificant at 0.5 Gy, at other doses, their quantities in female samples were at a significantly higher level than in male samples. Radiation induced transcription of Ier5 of females samples had a reduction in comparison with male samples at 1 and 2 Gy, but at doses of 0.5 and 4 Gy, females were significantly more susceptible to radiation-induced transcriptional alteration of Ier5. (authors)

  10. Protective and/or recovering effects of various kinds of chemicals and drugs to the hemopoietic injuries caused by the irradiation of /sup 60/Co. gamma. -rays in the mice

    Energy Technology Data Exchange (ETDEWEB)

    Kagimoto, Akio

    1987-01-01

    We have injected eleven kinds of chemical substances and drugs intraperitoneally in the male ddN mice, and studied the relative protective and/or recovering effects of them to the hemopoietic injuries caused by the whole body irradiation of 600R of /sup 60/Co ..gamma..-rays. Good radioprotective activity on bone marrow cells in the irradiated mice was found, when we administered AET (S, 2-aminoethylisothiuronium Br. HBr) before irradiation, 5-HTP (5-hydroxytryptophane) in low dosage before irradiation, Glutathione before irradiation, or Serotonin (5-HT; 5-hydroxytryptamine) in high dosage before irradiation. Good radioprotective or recovering activity was observed on the weight of the spleen, by Serotonin in high and low dosage before irradiation, or DBCC (5,6-dimethyl benzimidazolyl cobamide coenzyme; Vitamin B/sub 12/) after irradiation. Positive responses of reticulocytes, erythrocytes, hemoglobin, and hematocrit were obtained in the irradiated mice, when we administered Serotonin in high dosage before irradiation, MET (S-Methyl-l-cysteine sulfoxide) before irradiation, a cocktail of Periactin (Cyproheptadine hydrochloride) and Serotonin before irradiation, MET before and after irradiation or Nucleo (a mixture of products made by degrading yeast-RNA) after irradiation respectively. A good response in leukocyte count was observed when Serotonin in high dosage before irradiation was administered, and in granulocyte count by Serotonin in high dosage before or 5-HTP in low dosage before irradiation. Lymphocyte count was protected or recovered by Serotonin in high dosage before or Nucleo after irradiation. Thrombocyte count was protected by Serotonin in high and low dosage before, Glutathione before, or AET before irradiation.(author).

  11. Development of an analytical model for the determination of {sup 60}Co in aqueous samples by atomic absorption and gamma spectrometry techniques; Elaboracion de un modelo analitico para la determinacion de {sup 60}Co en muestras acuosas mediante tecnicas de absorcion atomica y espectrometria gamma

    Energy Technology Data Exchange (ETDEWEB)

    Solis A, N. A.

    2015-07-01

    The applications of the nuclear energy in industry and medicine generate radioactive wastes that must be isolated and confined in order to limit its spread in the biosphere. These types of wastes are generated in hospitals, industry, research centers and nuclear power plants (during de fuel cycle). The radioactive elements (radionuclides) cannot be destroyed by any known method, either chemical or mechanical. Its final destruction is produced by radioactive decay, which makes them stable isotopes, or nuclear transmutation being bombarded with atomic particles. Consequently, the radioactive waste management is to control the radioactive discharges and reduce to tolerable limits, eliminating of effluents and wastes the radionuclides of interest, concentrating them so they can be stored or evacuated so that later not appear in dangerous concentration in the biosphere. In Mexico, the main generators of radioactive wastes are the nuclear power plant of Laguna Verde in Veracruz, Hospitals of the public and private sector, in addition Instituto Nacional de Investigaciones Nucleares (ININ) some institutes and facilities of the UNAM and Instituto Politecnico Nacional (IPN) and industries among others. The aqueous radioactive wastes, particularly those from the nuclear power plants contain traces of cobalt-60. Currently there is no model or analytical technique for the separation of this radionuclide, so it became necessary to develop an analytical model for separation and facilitate their disposal. In this paper was shown that atomic absorption and gamma spectrometry techniques can separate the active concentration of the inactive, which is important because the total concentration can be associated through direct relationships with the wear of metal parts of the nuclear reactor, since the metal alloys of the same reactor containing a fraction of cobalt. Also this analytical and mathematical model that can be reproducible and applicable to full sets of samples and that this

  12. Estudo do efeito da irradiação gamma (60CO na qualidade da cachaça e no tonel de envelhecimento Study of the effect of gamma irradiation (60co on the quality of sugar cane spirit and on the cask of maturation

    Directory of Open Access Journals (Sweden)

    Mariana Branco de Miranda

    2006-12-01

    Full Text Available O processo de envelhecimento ou maturação das bebidas proporciona uma melhora nas características sensoriais da cachaça, tornando-a de qualidade superior e de maior valor econômico. O método tradicional de maturação de bebidas é sua interação com madeiras, sendo que a irradiação pode acelerar este processo de envelhecimento. A cachaça e os tonéis de carvalho de 20 L de capacidade foram submetidos à irradiação gama (150 Gy. Análises físico-químicas e cromatográficas foram realizadas periodicamente ao longo de 390 dias do período de envelhecimento da bebida. A irradiação da cachaça e do tonel não alterou a maioria dos componentes voláteis do coeficiente de congêneres como acidez volátil, ésteres, álcoois superiores e furfural durante os 390 dias. Há evidências, entretanto, de que os parâmetros de alguns componentes como aldeídos, taninos, cor e teor de cobre são de alguma forma influenciados, resultando em aceleração parcial do processo de maturação ou envelhecimento. Ao final do período de envelhecimento, foi feita uma análise sensorial com 30 provadores não treinados. A aceleração do processo de envelhecimento foi confirmada pela avaliação sensorial, e a cachaça e/ou tonel irradiados receberam maior indicação de aprovação em todos os parâmetros analisados (aroma, sabor e aparência.The ageing or maturing process of drink improves the sensorial characteristics of the product making it a qualified drink with higher economical value. The traditional maturing method of drinks involves their interaction with wood and irradiation can accelerate this ageing process. Sugar cane spirit and oak barrels with a 20 L capacity were treated using gamma irradiation (150 Gy. Physical-chemical and chromatographic analyses were constantly performed for 390 days during the drink ageing period. The sugar cane spirit and barrel irradiation did not change most volatile components of the same type coefficient such

  13. Effect of gamma radiation from {sup 60}C in conservation and quality of pepper fresh and pulp; Efeito da radiacao gama do {sup 60}Co na conservacao e qualidade de pimenta in natura e em polpa

    Energy Technology Data Exchange (ETDEWEB)

    Milagres, Regina Celia Rodrigues de Miranda

    2014-07-01

    Capsicum peppers are among the most used and most valued seasoning spices in the world. They are highly susceptible to post harvest decay, therefore the use of irradiation may contribute to conservation of this fruit which has significant nutritional, economic and social value. Were evaluated the effects of gamma radiation of {sup 60}Co in increasing the shelf-life and preservation of quality of pepper 'Dedo-de-moca' (Capsicum baccatum var. Pendulum) fresh and pulp associated or not with another conservation methods. Gamma radiation doses were investigated of 0,25; 0,50; 0,75; 1,00; 1,25; 1,50; 2,00 e 3,00 kGy in fresh fruit and 1,50; 2,00; 3,00; 4,50 e 6,00 kGy in pepper pulp. The use of laboratory pasteurization (85°C/3min), the addition of citric acid (5%) and 5% NaCl were also investigated in the pepper pulp. The samples were stored at 5 °C and or 25 °C. Were performed analysis: visual (disease incidence, turgidity and color); bioactive compounds (carotenoids, capsaicinoids, antioxidant capacity and phenolic compounds); physicochemical (Weight loss, firmness, soluble solids (SS), pH, titratable acidity (TA), ratio, color and moisture); respiratory rate and ethylene production; proximate composition (moisture, soluble and insoluble fiber, ash, protein, ether extract, total and available carbohydrate); microbiological contamination (mesophilic aerobic microorganisms, coliforms at 45 °C, Salmonella spp. and yeasts and molds) and sensory (acceptance test, difference test and paired preference). Radiation doses between 0.25 and 3.00 kGy were not effective to increase shelf-life and maintain fresh pepper quality during storage. There was increased intensity of red color, of incidence of disease, decreased turgidity and changes in physical, chemical and nutritional parameters. In pulp, the doses of gamma radiation of 4.50 and 6.00 kGy and the addition of 5% NaCl no changing sensory attributes, physical-chemical and bioactive compounds. Only the

  14. Experience of the accomplishment of dismantling of a radiation gamma facility with Co-60 source; Experiencia de la realizacion de un cierre definitivo de una instalacion de irradiacion gamma con fuente de {sup 60} Co

    Energy Technology Data Exchange (ETDEWEB)

    Perez Reyes, Yolanda; Hernandez Alvarez, Ramon; Sarabia Molina, Igor I.; Jerez Vegueria, Pablo [Centro Nacional de Seguridad Nuclear, La Habana (Cuba)

    2001-07-01

    The present work describes the experience obtained by the specialists of the Cuban Regulatory Authority, as a result of the evaluation of observance, criteria of security and radiological safety, during the stage conducted in the works of dismantling of a gamma irradiator.

  15. Metabolism of 60Co in mollusca

    International Nuclear Information System (INIS)

    Nakahara, Motokazu

    1983-01-01

    After mollusca were bred in 60 Co-labeled sea water or were given 60 Co-labeled feed for certain hours, their tissues including the liver were removed as experimental samples. 60 Co metabolism was observed by the Sephadex gel filtration profile. A gel chromatogram of the liver in gastropoda showed a marked peak of 60 Co on the high-molecular side. Although the peak was observed on both high- and low-molecular sides in pelecypoda, it was more marked on the high-molecular side than on the low-molecular side. In cephalopoda, the peak pattern was similar to that in the other mollusca, but the proportion of low-molecular components was comparatively large. The gel filtration profile of 60 Co in various tissues of cephalopoda revealed the incorporation of 60 Co into the high-molecular components in blood, three peaks in the kidneys, and the incorporation of 60 Co into the low-molecular components in the branchial heart. The metabolism of 60 Co was dependent upon the chemical form of Co in gastropoda, and organic 60 Co was specifically observed in the high-molecular components. (Namekawa, K.)

  16. Determination of the correction factor for attenuation, dispersion and production of electrons (Kwall) in the wall of graphite of a ionization chamber Pattern National Type CC01 in fields of gamma radiation of 60Co

    International Nuclear Information System (INIS)

    Alvarez R, J.T.; Morales P, J.; Cruz E, P.

    2001-12-01

    It was determined the Kwall correction factor for the wall of graphite of the chamber of the pattern national type CC01 series 133 for a radiation field Gamma of 60 Co. With this end to measured the currents of ionization l(x) as function of the thickness of the wall of the chamber: X=4,8,12,16 and 20 mm.The mensurations for each thickness consisting of three groups, of sizes n = 30 or 60 data for each group; obtaining 8 complete groups of mensurations independent in eight different dates.The determinate the factor carried out using three regression models: lineal, logarithmic and quadratic, models that were tried to validate with the tests of : i) Shapiro-Wilk and χ 2 for the normality of the entrance data ii) Tests of Bartlett for variances homogeneity among groups for each thickness iii) The tests of Duncan for the stockings among groups of each thickness, and iv) The tests of adjustment lack (LOF) for the models used. Nevertheless, alone the models of the group of corresponding mensurations at 01-03-2000 17-08-2001 they can be validated by LOF, but not for tests of normality and homogeneity of variances. Among other assignable causes of variation we have: i) The values captured by the system of mensuration of the variables of it influences: pressure, temperature and relative humidity don t belong together with the existent ones to the moment to capture the l(x). ii) The mensuration room presents flows of air, for what was suited o diminish their volume and to eliminate the flows of air. iii) A protocol settled down of taking of measures that it consisted in: - Pre-irradiation 5 minutes the chamber after the change of polarity and hood change, with a period of stabilization of 5 minutes after the pre-irradiation. - Pre-irradiation for 5 minutes before the taking of the readings, with the object of eliminating variation sources assigned to currents of escapes or due variations to transitory. iv) To realize corrections for relative humidity of agreement with the

  17. Relative Biological Effectiveness of 14-MeV Fast Neutrons to Co{sup 60} Gamma-Rays in Einkorn Wheat; Efficacite Biologique Relative des Neutrons Rapides de 14 MeV par Rapport aux Rayons Gamma de {sup 60}Co sur l'Engrain; Otnositel'naya biologicheskaya ehffektivnost' bystrykh nejtronov s ehnergiej 14 MeV i gamma-luchej CO{sup 60} pri ikh dejstvii na pshenitsu odnozernyanku; EBR de los Neutrones Rapidos de 14 MeV y de los Rayos Gamma del {sup 60}Co en el Trigo Escanda Menor

    Energy Technology Data Exchange (ETDEWEB)

    Fujii, T. [National Institute of Genetics, Misima (Japan)

    1964-05-15

    somatic mutation. (author) [French] L' auteur a etudie l'EBR des neutrons de 14 MeV par rapport aux rayons gamma de {sup 60}Co en appliquant a l'engrain la methode des loci specifiques. Il a utilise pour cette etude des semences Fi resultant du croisement entre l'espece originale et un mutant chlorina (le mutant chlorina e ta it le produit d'une mutation recessive induite par les rayons X ; depuis le stade de plantule jusqu'a sa maturite, le m utant a conserve une couleur vert c la ir uniforme ainsi qu'un taux de survie et une fe rtilite relativement eleves). Les plantes Fi avaient une couleur verte et une croissance normales. Des semences dormantes de F{sub 1} ont e te irradiees avec 0,5 , 1,0 et 1,4 krad de neutrons rapides et avec 4,3 , 8,6 et 12,9 krad de rayons gamma. Sous l'effet des deux types de rayonnements, il s'est produit des mutations de vert normal dominant en chlorina, sous forme de bandes longitudinales. Sur les feuilles et les tiges des heterozygotes X{sub 1}. Environ 80% des semences ont germe dans le lot temoin ainsi que dans les deux lots ayant recu la plus faible dose de neutrons et de rayons gamma; les taux de germination ont diminue a mesure qu'augmentaient les doses de neutrons et de rayons gamma. En outre, on a observe un phenomene analogue au debut de lacroissance de la plantule, qui etait progressivement inhibee a mesure qu'augmentait la dose de rayonnement. Les resultats obtenus montrent que l'irradiation par les neutrons est environ 13 fois plus efficace que celle par les rayons gamma. Le taux de survie des semences non irradiees du lo t temoin a e te de 90% environ; 60 a 80% des plantules ont survecu aux doses de 0,5 et 1,0 krad de neutrons; toutes les plantilles irradiees par les rayons gamma ont survecu. En revanche, 4% seulement des plantules ont survecu a 1,4 krad de neutrons. L'auteur n 'a pas observe de mutations chez les plantes du lo t temoin; le nombre des plantes a talles rayees a augmente en meme temps que les doses des deux types

  18. Changes in lens following 60Co irradiation

    International Nuclear Information System (INIS)

    Rozsival, P.; Obenberger, J.; Sladkova, J.

    1985-01-01

    Chinchilla rabbit lenses were irradiated using a 60 Co gamma source, and the changes in the transport activities, native weight, water content and dry weight were observed. The heads of rabbits were irradiated with doses of 10 and 20 Gy and functional changes occurring in the lenses were studied till the twenty-fourth week after the irradiation. The weight of the rabbit lens irradiated with a dose of 20 Gy significantly increased after the twelfth week and was maximal 16 weeks after irradiation when also cataracts were observed. The increased weight of lenses was caused by accumulation of water. The decrease in the dry weight of lenses preceded the period of visible cataracts. A decreased uptake of rubidium occurring during in vitro cultivation of lenses was the most sensitive marker for the changes caused by 20 Gy irradiation. It was significantly decreased as early as 8 weeks after irradiation, preceding the increased hydration of lenses by 4 weeks and the presence of visible cataracts by as much as 8 weeks

  19. Characterization of 60Co gamma-radiation induced radical products of antipyrine by means of high-performance liquid chromatography, mass spectrometry, capillary zone electrophoresis, micellar electrokinetic capillary chromatography and nuclear magnetic resonance spectrometry

    NARCIS (Netherlands)

    Coolen, S.A.J.; Everaerts, F.M.; Huf, F.A.

    1997-01-01

    Monitoring the amount of oxidative damage, caused by free radicals, is a major problem in free radical and aging research. Antipyrine is proposed as an exogenous marker for the biomolecular monitoring of oxidative stress. In this paper the characterization of the 60Co ¿-radiation products of

  20. Determination of the correction factor for attenuation, dispersion and production of electrons (K{sub wall}) in the wall of graphite of a ionization chamber Pattern National Type CC01 in fields of gamma radiation of {sup 60}Co; Determinacion del factor de correccion por atenuacion, dispersion y produccion de electrones (K{sub wall}) en la pared de grafito de una Camara de Ionizacion Patron Nacional Tipo CC01 en campos de radiacion gamma de {sup 60} Co

    Energy Technology Data Exchange (ETDEWEB)

    Alvarez R, J.T.; Morales P, J.; Cruz E, P. [ININ, 52045 Ocoyoacac, Estado de Mexico (Mexico)

    2001-12-15

    It was determined the Kwall correction factor for the wall of graphite of the chamber of the pattern national type CC01 series 133 for a radiation field Gamma of {sup 60}Co. With this end to measured the currents of ionization l(x) as function of the thickness of the wall of the chamber: X=4,8,12,16 and 20 mm.The mensurations for each thickness consisting of three groups, of sizes n = 30 or 60 data for each group; obtaining 8 complete groups of mensurations independent in eight different dates.The determinate the factor carried out using three regression models: lineal, logarithmic and quadratic, models that were tried to validate with the tests of : i) Shapiro-Wilk and {chi}{sup 2} for the normality of the entrance data ii) Tests of Bartlett for variances homogeneity among groups for each thickness iii) The tests of Duncan for the stockings among groups of each thickness, and iv) The tests of adjustment lack (LOF) for the models used. Nevertheless, alone the models of the group of corresponding mensurations at 01-03-2000 17-08-2001 they can be validated by LOF, but not for tests of normality and homogeneity of variances. Among other assignable causes of variation we have: i) The values captured by the system of mensuration of the variables of it influences: pressure, temperature and relative humidity don{sup t} belong together with the existent ones to the moment to capture the l(x). ii) The mensuration room presents flows of air, for what was suited o diminish their volume and to eliminate the flows of air. iii) A protocol settled down of taking of measures that it consisted in: - Pre-irradiation 5 minutes the chamber after the change of polarity and hood change, with a period of stabilization of 5 minutes after the pre-irradiation. - Pre-irradiation for 5 minutes before the taking of the readings, with the object of eliminating variation sources assigned to currents of escapes or due variations to transitory. iv) To realize corrections for relative humidity of

  1. 60Co levels in the seawater regions

    International Nuclear Information System (INIS)

    Yoshioka, Mitsuo

    1983-01-01

    In order to assess the contribution from nuclear power facilities to the 60 Co levels in seawater regions, it is essential to grasp the background values of 60 Co. The following matters are described: 60 Co sources and the respective inputs; nuclear test fallout and nuclear power plants; the 60 Co levels in overseas countries; the 60 Co levels in Japan; the 60 Co levels from nuclear power plants in Fukui prefecture. In the seawater regions around Japan, there have been numerous instances of 60 Co detection; several pCi/kg of dry earth in sea bottom earth and about 1 pCi/kg of raw material in marine life can be considered as the background levels due to nuclear test fallout and nuclear-powered submarines. In the seawater regions of Fukui prefecture, the 60 Co levels appreciably exceeded the above background due to the nuclear power plants, which are insignificant concerning the radiation exposure of the local people. (Mori, K.)

  2. Study of the action of {sup 60}Co gamma radiation on Salmonella poona, Escherichia coli and Alicyclobacillus acidoterrestris in mango pulp; Estudo da acao da radiacao gama de {sup 60}Co sobre Salmonella poona, Escherichia coli e Alicyclobacillus acidoterrestris em polpa de manga congelada

    Energy Technology Data Exchange (ETDEWEB)

    Pereira, Marco Antonio dos Santos

    2009-07-01

    The application of non-thermal treatments has proven effective in inhibiting bacteria such as Salmonella and Escherichia coli. Mango is a fruit of national consumption with a great exportation potential. Meanwhile, outbreaks of food borne disease related to mango consumption caused mistrust on the degree of food security offered by the product. The objective of this work was to establish the radioresistance of bacteria Escherichia coli, Salmonella Poona and Alicyclobacillus acidoterrestris on the mango pulp by the calculation of the D10 values and to know the radiation effect on the sensory characteristics of the fruit pulp. The microbiological profile of frozen mango pulp available at the local market was also established using conventional methods of plating and Most Probable Number (MPN). The pulps experimentally inoculated with the bacteria listed above were irradiated with doses of 0, 1, 2, 3, 4 and 5kGy in a {sup 60}Co source. The sensory analysis was performed using a dose of 5 kGy, using the triangular test and the test of acceptance with hedonic scale. The results of this study show that the quality of mango pulp sell in the local market is not satisfactory in accordance with the standards established by the Brazilian law and the literature, showing the need of using other tools to achieve acceptable levels of quality. The D10 values obtained are in the range of 1.01 and 1.09kGy for E. coli ATCC 8739, 0.60 and 0.98kGy for S. poona and 0.72 e 0.88kGy for A. acidoterrestris respectively. The triangular test showed that a 5kGy radiation dose changed the sensory characteristics of mango pulp. Nevertheless, sensory analysis of a food product prepared with the irradiated pulp obtained good acceptance in the attributes of global appearance, flavor and aroma. (author)

  3. Study of the action of {sup 60}Co gamma radiation on Salmonella poona, Escherichia coli and Alicyclobacillus acidoterrestris in mango pulp;Estudo da acao da radiacao gama de {sup 60}Co sobre Salmonella poona, Escherichia coli and Alicyclobacillus acidoterrestris em polpa de manga congelada

    Energy Technology Data Exchange (ETDEWEB)

    Pereira, Marco Antonio dos Santos

    2009-07-01

    The application of non-thermal treatments has proven effective in inhibiting bacteria such as Salmonella and Escherichia coli. Mango is a fruit of national consumption with a great exportation potential. Meanwhile, outbreaks of foodborne disease related to mango consumption caused mistrust on the degree of food security offered by the product. The objective of this work was to establish the radioresistance of bacteria Escherichia coli, Salmonella Poona and Alicyclobacillus acidoterrestris on the mango pulp by the calculation of the D10 values and to know the radiation effect on the sensory characteristics of the fruit pulp. The microbiological profile of frozen mango pulp available at the local market was also established using conventional methods of plating and Most Probable Number (MPN). The pulps experimentally inoculated with the bacteria listed above were irradiated with doses of 0, 1, 2, 3, 4 and 5 kGy in a {sup 60}Co source. The sensory analysis was performed using a dose of 5 kGy, using the triangular test and the test of acceptance with hedonic scale. The results of this study show that the quality of mango pulp sell in the local market is not satisfactory in accordance with the standards established by the Brazilian law and the literature, showing the need of using other tools to achieve acceptable levels of quality. The D10 values obtained are in the range of 1.01 and 1.09 kGy for E. coli ATCC 8739, 0.60 and 0.98 kGy for S. poona and 0.72 and 0.88 kGy for A. acidoterrestris respectively. The triangular test showed that a 5 kGy radiation dose changed the sensory characteristics of mango pulp. Nevertheless, sensory analysis of a food product prepared with the irradiated pulp obtained good acceptance in the attributes of global appearance, flavor and aroma. (author)

  4. 60Co Transfer Factor From Lutosol Soil To Leafy Vegetable (Ipomea reptans poir)

    International Nuclear Information System (INIS)

    Leli-Nirwani; Jumaher; Wahyudi

    2003-01-01

    It has been conducted 60 Co transfer study from Lutosol soil to leafy vegetable plant by using pot treatment system. The aim of the research is to obtain data of the transfer factor of 60 Co from lutosol soil to leafy vegetable plant. Pot experiment was carried out by using the complete random design to evaluate two treatment, namely soil with 60 Co and without 60 Co (as a control). 60 Co concentration was with 6.1371 kBq/pot. Tested plant and control plant amount to 12 pots. Each pot contains 1 kg of soil. After harvesting, the weight of dry plant was determined and was conducted with Spectrometer Gamma to 60 Co concentration in dry plant and dry soil. Transfer factor was found to between 0.02 and 0.68 and the average is 0.2068. (author)

  5. Effects of the gamma and ultrasound radiation in orange juice contaminated by Alicyclobacillus acidoterrestris;Efeitos das radiacoes gama e ultra-sonica em suco de laranja contaminado por Alicyclobacillus acidoterrestris

    Energy Technology Data Exchange (ETDEWEB)

    Pires, Cristiane Cassiolato, E-mail: cristiane_cassiolato@yahoo.com.b

    2006-07-01

    The orange juice has attributes could make that hard or even block the growth. of microorganisms, even so already have been isolated filamentous fungi, yeast, as well lactic and heat resistant bacteria. Alicyclobacillus acidoterrestris is a heat resistant bacteria that has done damage in the orange juice concentrated and frozen business. This bacterium is able the grown in temperatures below 35 deg C constituting a risk to orange juices that have been contaminated. Even those that already pass trough thermal treatment may deteriorated, if they are storage in place without refrigeration. As the traditional methods of decontamination and conservation have not been effective in unfeasible this bacterium, others methods of sterilization are need, among them are gamma and ultrasound radiation. The gamma radiation is able to sterilize foods and reduce the microbiology density, allowing in this way to enhance the period of storage. The ultrasound application has been used currently with the aim to microbiology control, been this technique effective in the microbial cell destruction. The Brazil, are the major producer and exporter of concentrated frozen orange juice, due to it, the present work was carried out to two experiments with the aim to determinate the resistance of the bacterium Alicyclobacillus acidoterrestris to gamma and ultrasound irradiation, in the orange juice, priory contaminated. The orange juice samples was diluted to 11,5 deg Brix and the bacteria suspensions was added, passed by process of radiation gamma with dose of 0, 2, 4, 6 and 8 kGy and ultrasonic with the frequency of 25, 35 and 42 kHz and with the time of exposition of 0, 1, 5, 10 and 20 minutes. After the treatment, the samples were stored in room temperature (25 +- 2 deg C) and of refrigeration (4 +- 1 deg C). For the microbial analyses, the samples were diluted in to decimal scale, plated by 'pour plate' technique. The Petri plates were storage in warn temperature (46 +- 2 deg C

  6. Description of morphologic characters in avocado trees (Persea americana. Mill) var. Hass, submitted to gamma radiation of {sup 60} Co; Descripcion de caracteres morfologicos en arboles de aguacate (Persea americana. Mill) var. Hass, sometidos a radiacion gama de {sup 60} Co

    Energy Technology Data Exchange (ETDEWEB)

    Sandoval R, L

    2004-07-01

    It was carried out the characterization of 77 trees of avocado ''Hass'' subjected to gamma radiation of Co{sup 60}, in doses of 0, 5, 10,15, 20 and 25 Gy based on previous studies of radiosensitivity with ends of improvement located in the experimental center ''La Labor'' belonging to the foundation Salvador Sanchez Colin. CICTAMEX. S C. in Temascaltepec, Mexico. It was carried out the morphological characterization of this variety according to the nomenclature settled down by the International Office of Genetic Resources (IBPGRI), registering 27 variables for the description of the tree, 33 for description of the fruit and 14 for the characterization of the seed. Regarding the architectural characterization 10 variables were evaluated. Using gamma radiation of Co{sup 60} in the doses of 15 and 20 Gy it was detected a reduction of 22% in the portage of the tree, likewise had reduction the diameter of the trunk and of the foliage. It was found that the reduction in the behavior of the tree induces to a bigger variation of the form of the same one, in the distribution of the branches in the form of the fruit and in the form of the seed you Also detects a remarkable increment in the productivity of some hoist irradiated regarding the architecture of the tree one observes that the angle of inclination of the branches increase when being increased the radiation dose and that the longitude of the annual section of growth decreased significantly in the doses of 20 and 25 Gy. One observes a significant Increment in the I number of buds prolepticos in those hoist irradiated in the I finish mbdulo of rhythmic growth. Io that indicates a change substantial in the I inhabit of growth With the purpose of determining if the changes in morphology and architecture of the tree corresponded with the modification at level meristematico, they were carried out observations to the court microscope of having knitted apical vegetative meristematico in samples of you hoist of all the

  7. Description of morphologic characters in avocado trees (Persea americana. Mill) var. Hass, submitted to gamma radiation of {sup 60} Co; Descripcion de caracteres morfologicos en arboles de aguacate (Persea americana. Mill) var. Hass, sometidos a radiacion gama de {sup 60} Co

    Energy Technology Data Exchange (ETDEWEB)

    Sandoval R, L

    2004-07-01

    It was carried out the characterization of 77 trees of avocado ''Hass'' subjected to gamma radiation of Co{sup 60}, in doses of 0, 5, 10,15, 20 and 25 Gy based on previous studies of radiosensitivity with ends of improvement located in the experimental center ''La Labor'' belonging to the foundation Salvador Sanchez Colin. CICTAMEX. S C. in Temascaltepec, Mexico. It was carried out the morphological characterization of this variety according to the nomenclature settled down by the International Office of Genetic Resources (IBPGRI), registering 27 variables for the description of the tree, 33 for description of the fruit and 14 for the characterization of the seed. Regarding the architectural characterization 10 variables were evaluated. Using gamma radiation of Co{sup 60} in the doses of 15 and 20 Gy it was detected a reduction of 22% in the portage of the tree, likewise had reduction the diameter of the trunk and of the foliage. It was found that the reduction in the behavior of the tree induces to a bigger variation of the form of the same one, in the distribution of the branches in the form of the fruit and in the form of the seed you Also detects a remarkable increment in the productivity of some hoist irradiated regarding the architecture of the tree one observes that the angle of inclination of the branches increase when being increased the radiation dose and that the longitude of the annual section of growth decreased significantly in the doses of 20 and 25 Gy. One observes a significant Increment in the I number of buds prolepticos in those hoist irradiated in the I finish mbdulo of rhythmic growth. Io that indicates a change substantial in the I inhabit of growth With the purpose of determining if the changes in morphology and architecture of the tree corresponded with the modification at level meristematico, they were carried out observations to the court microscope of having knitted apical vegetative meristematico

  8. Sorption of 60 Co on inorganic solids

    International Nuclear Information System (INIS)

    Granados C, F.; Bulbulian G, S.; Mardel V, B.

    2003-01-01

    The behavior of sorption of the 60 Co in aqueous solution under static conditions to different values of pH of the aqueous solution (1, 3, 5, 7, and 10) on MgO, MnO 2 , SnO, TiO 2 , activated carbon and calcinate hydrotalcite was investigated. It was found that the best sorbents of the 60 Co was the MnO 2 , activated carbon and TiO 2 whose sorption was incremented when increasing the pH value of the aqueous solutions, in the one case of the hydrated oxides, the 60 Co interacted with the electrically charged surface of the sorbents that depends on the pH of the solution and of the point of zero charge (zpc) of the sorbent. (Author)

  9. Modeling of 60Co transport by groundwater

    International Nuclear Information System (INIS)

    Serebryakov, B.E.; Ivanov, E.A.; Shchukin, A.P.

    2006-01-01

    Results of calculation of the 60 Co migration in a water-bearing horizon after the accident Novo-Voronezh NPP in 1985 are presented. The accident was connected with the liquid radioactive waste leakage into the ground water. The features of 60 Co migration in the water-bearing horizon and its coming into the Don river are calculated by means of three-dimensional models. Population irradiation estimations show that the accident did not lead to exceeding the recommended dose limits [ru

  10. Action of Bothrops moojeni venom and its L-amino acid oxidase fraction, treated with {sup 60}Co gamma rays, in Leishmania spp; Acao do veneno de Bothrops moojeni e sua fracao L-aminoacido oxidase, submetida ao tratamento com raios gama de {sup 60}Co, em Leishmania spp

    Energy Technology Data Exchange (ETDEWEB)

    Cardoso, Andre Gustavo Tempone

    1999-07-01

    Bothrops moojeni venom showed an anti leishmania activity in vitro, as determined by a cell viability assay using the reduction of MTT. After venom purification, by chromatography techniques, the fractions with anti leishmania and L-amino acid oxidase activities, eluted in the same positions. The molecular weight of the enzyme was estimated to be 140 kDa by molecular exclusion chromatography, and 69 kDa, by SDS-PAGE, migrating as a single band, with an isoelectric point of 4.8 as determined by isoelectric focusing. The purified LAO from B. moojeni venom, 135-fold more active than crude venom, showed homo dimeric constitution, and was active against Leishmania spp from the New World, with an effective concentration against L(L). amazonensis of 1.80 {mu}g/ml (EC{sub 50}), L.(V.) panamensis (0.78 |{mu}g/ml) and L.(L.) chagasi (0.63 ({mu}g/ml). Ultrastructural studies of promastigotes affected by LAO demonstrated cell death, with edema in several organelles such as mitochondria and nuclear membrane, before cell disruption and necrosis. The action of LAO was demonstrated to be hydrogen peroxide-dependent. Studies with LLCMK-2 cells, treated with LAO, showed a toxic effect, with an EC{sub 50} of 11|{mu}g/ml. Irradiation of LAO with 6{sup 0C}o gamma rays, did not affect its whole oxidative activity, neither detoxified the enzyme. Amastigotes treated with LAO were not affected by its hydrogen peroxide, otherwise, the exogenous product, killed amastigotes with an EC{sub 50} of 0.67mM. These data could be of help in the development of alternative therapeutic approaches to the treatment of leishmaniasis. (author)

  11. Determination of 60Co sorption in natural clinoptilolite

    International Nuclear Information System (INIS)

    Hernandez B, E.; Granados C, F.

    1997-01-01

    It was studied the clinoptilolite behavior coming from a deposit in Taxco, Guerrero in hydration and stabilization conditions with sodium for determining its sorption properties. The ion exchange process was carried out through gamma spectrometry using a CoCl 2 solution marked with 60 Co at p H 6.5 in different contact times. It was observed a maximum sorption of 0.408 m eq Co +2 /g mineral, from 0.314 m eq Co +2 /g mineral correspond at ion exchange. (Author)

  12. Determination of {sup 60}Co sorption in natural clinoptilolite; Determinacion de la sorcion de {sup 60}Co en clinoptilolita natural

    Energy Technology Data Exchange (ETDEWEB)

    Hernandez B, E.; Granados C, F. [Instituto Nacional de Investigaciones Nucleares, A.P. 18-1027, 11801 Mexico D.F. (Mexico)

    1997-07-01

    It was studied the clinoptilolite behavior coming from a deposit in Taxco, Guerrero in hydration and stabilization conditions with sodium for determining its sorption properties. The ion exchange process was carried out through gamma spectrometry using a CoCl{sub 2} solution marked with {sup 60} Co at p H 6.5 in different contact times. It was observed a maximum sorption of 0.408 m eq Co{sup +2}/g mineral, from 0.314 m eq Co{sup +2}/g mineral correspond at ion exchange. (Author)

  13. Effect of ethanolic extract of propolis on cell viability of chinese hamster ovary cells (CHO-K1) irradiated with {sup 60}CO gamma-rays using differential staining technique

    Energy Technology Data Exchange (ETDEWEB)

    Castro, Marcos P.M. de; Castro, Renato F. de; Okazaki, Kayo; Vieira, Daniel P., E-mail: dpvieira@ipen.br [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)

    2013-07-01

    The objective of present study was to assess the effect of Brazilian propolis (AF-08) on CHO-K1 cells irradiated with {sup 60}Co, through the differential staining technique, using acridine orange and ethidium bromide. The cells were pre-incubated with different concentrations of propolis (50, 100 and 200 μg/mL) for 24h and irradiated with 5 Gy, analyzed at 24 and 48h after exposure. This technique is based on the cell capacity to incorporate fluorescent DNA dyes, where the viable (green), apoptotic (orange/yellow) and necrotic (red) cells can be identified through fluorescence microscopy. Digital high-resolution images were acquired from at least 5 visualization fields, and cells were analyzed using ImageJ and Flowing software. This approach permitted to analyze a large number of cells/sample with the time reduction, much easier and faster, proportioning more statistical power of the technique. The treatment with propolis only was not cytotoxic at 24 and 48h, except for the higher concentration of 200 μg/mL associated or not with radiation, increasing apoptotic and mainly necrotic cells (p<0.001). The data showed a promising use of propolis as well as technique used, pointing out that 200 μg/mL of propolis was cytotoxic, but at lower one (50 μg/mL) presented a radioprotective effect in irradiated CHO-K1 cells. (author)

  14. Effect of ethanolic extract of propolis on cell viability of chinese hamster ovary cells (CHO-K1) irradiated with 60CO gamma-rays using differential staining technique

    International Nuclear Information System (INIS)

    Castro, Marcos P.M. de; Castro, Renato F. de; Okazaki, Kayo; Vieira, Daniel P.

    2013-01-01

    The objective of present study was to assess the effect of Brazilian propolis (AF-08) on CHO-K1 cells irradiated with 60 Co, through the differential staining technique, using acridine orange and ethidium bromide. The cells were pre-incubated with different concentrations of propolis (50, 100 and 200 μg/mL) for 24h and irradiated with 5 Gy, analyzed at 24 and 48h after exposure. This technique is based on the cell capacity to incorporate fluorescent DNA dyes, where the viable (green), apoptotic (orange/yellow) and necrotic (red) cells can be identified through fluorescence microscopy. Digital high-resolution images were acquired from at least 5 visualization fields, and cells were analyzed using ImageJ and Flowing software. This approach permitted to analyze a large number of cells/sample with the time reduction, much easier and faster, proportioning more statistical power of the technique. The treatment with propolis only was not cytotoxic at 24 and 48h, except for the higher concentration of 200 μg/mL associated or not with radiation, increasing apoptotic and mainly necrotic cells (p<0.001). The data showed a promising use of propolis as well as technique used, pointing out that 200 μg/mL of propolis was cytotoxic, but at lower one (50 μg/mL) presented a radioprotective effect in irradiated CHO-K1 cells. (author)

  15. Effect of neutrons with an energy of 0.35 and 0.85 MeV and gamma radiation of 60Co on the organ cultures of the human bone marrow

    International Nuclear Information System (INIS)

    Zherbin, E.A.; Kolesnikova, A.I.; Konoplyannikov, A.G.; Khoptynskaya, S.K.; Obaturov, G.M.; Kapchigashev, S.P.

    1979-01-01

    A comparative study of the radiation injury of human hemopoietic cells under the effect of γ radiation of 60 Co in doses from 100 to 1500 rad, 0.85 MeV neutrons in doses from 50 to 600 rad and 0.35 MeV neutrons in doses from 30 to 600 rad was carried out using the method of organic cultivation of bone marrow. Under the effect of the above radiation types, the fissionable granulocytes (promyelocytes and myelocytes) and lymphocytes are most radiosensitive among the 4-day organic cultures. The dose dependence of the decrease in relative and absolute content of these cell forms in the cultures as well as the dose dependence of the decrease in total cell structure in the cultures were revealed. It has been found that the dose dependence for the above tests consists of two components: radiosensitive and radioresistant ones. The radiation doses causing death in 50% of cells in relation to control (D 50 ) were determined. On the bases of D 50 values, the coefficients of relative biological efficiency (RBE) were calculated for 0.85 and 0.35 MeV neutrons. The RBE coefficients are confined in the 1.4-3.4 and 2.4-6.7 intervals respectively. It has been confirmed that 0.35 MeV neutrons are 1.5-2 times more effective than 0.85 MeV neutrons. The coefficients of RBE neutrons calculated for radioresistant cell components are higher than the corresponding values estimated according to D 50 value; in some cases they reach values ranging from 6 to 10

  16. Development of irradiator 60Co sources

    International Nuclear Information System (INIS)

    Mosca, Rodrigo C.; Moura, Eduardo S.; Zeituni, Carlos A.; Mathor, Monica B.

    2011-01-01

    According to a recent report by the International Agency for Research on Cancer (IARC) / WHO (2008-2010), the global impact of cancer more than doubled in 30 years. In this report, it was estimated that occurred about 12 million new cancer cases and 7 million deaths. In Brazil in 2010, with estimates for the year 2011, point to the occurrence of 489,270 new cases of cancer. Among the possibilities for cancer treatment, radiotherapy is one of the most important therapeutic and resources used to combat it. However, inherent complications of treatment can occur such as tiredness, loss of appetite, radiodermatitis and in more extreme cases late radionecrosis. In order to reproduce a point of radionecrosis in the vicinity of radiodermatitis to mimic these effects in animals, producing a model for assessment of tissue repair, we propose the setting up of an irradiator source of collimated 60 Co. The development of was based on 11 sources of 60 Co with 1 mm thickness that were inserted by inference in stainless steel 'gate-source' screw (patent pending) and later adjusted in a cross-shaped arrangement reinforced so that the beam radiation is directed to a target point, saving for other regions around this target point. The main use of this irradiator with sources of 60 Co is just one cause radionecrosis point (target point) of approximately 5 mm 2 with a surrounding and adjacent area of radiodermatitis around about 8 to 10 mm 2 in laboratory animals for subsequent coating with epidermal-dermal matrix populated by a cell culture of human fibroblasts, keratinocytes and mesenchymal stem cells. With that said, its use will be valuable for evaluation of curative treatments against the bone and radionecrosis or palliative treatment rather than as it is currently assumed. (author)

  17. Development of irradiator {sup 60}Co sources

    Energy Technology Data Exchange (ETDEWEB)

    Mosca, Rodrigo C.; Moura, Eduardo S.; Zeituni, Carlos A.; Mathor, Monica B., E-mail: rcmosca@usp.b [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)

    2011-07-01

    According to a recent report by the International Agency for Research on Cancer (IARC) / WHO (2008-2010), the global impact of cancer more than doubled in 30 years. In this report, it was estimated that occurred about 12 million new cancer cases and 7 million deaths. In Brazil in 2010, with estimates for the year 2011, point to the occurrence of 489,270 new cases of cancer. Among the possibilities for cancer treatment, radiotherapy is one of the most important therapeutic and resources used to combat it. However, inherent complications of treatment can occur such as tiredness, loss of appetite, radiodermatitis and in more extreme cases late radionecrosis. In order to reproduce a point of radionecrosis in the vicinity of radiodermatitis to mimic these effects in animals, producing a model for assessment of tissue repair, we propose the setting up of an irradiator source of collimated {sup 60}Co. The development of was based on 11 sources of {sup 60}Co with 1 mm thickness that were inserted by inference in stainless steel 'gate-source' screw (patent pending) and later adjusted in a cross-shaped arrangement reinforced so that the beam radiation is directed to a target point, saving for other regions around this target point. The main use of this irradiator with sources of {sup 60}Co is just one cause radionecrosis point (target point) of approximately 5 mm{sup 2} with a surrounding and adjacent area of radiodermatitis around about 8 to 10 mm{sup 2} in laboratory animals for subsequent coating with epidermal-dermal matrix populated by a cell culture of human fibroblasts, keratinocytes and mesenchymal stem cells. With that said, its use will be valuable for evaluation of curative treatments against the bone and radionecrosis or palliative treatment rather than as it is currently assumed. (author)

  18. Microstructural study of Y{sub 1}Ba{sub 2}Cu{sub 3}O{sub 7-x}/Ag samples irradiated with {sup 60}Co {gamma} rays at high doses

    Energy Technology Data Exchange (ETDEWEB)

    Rangel, R [Programa de Postgrado en Fisica de Materiales, Centro de Investigacion Cientifica y de Educacion Superior de Ensenada, Apartado Postal 2681, CP 22800, Ensenada, BC (Mexico); Galvan, D H [CECIMAC-UNAM, Apartado Postal 2681, CP 22800, Ensenada, BC (Mexico); Adem, E [Instituto de Fisica-UNAM, Apartado Postal 20-364, CP 01000, Mexico DF (Mexico); Bartolo-Perez, P [CINVESTAV-IPN Unidad Merida, Departamento de Fisica Aplicada, Merida, Yucatan (Mexico); Maple, M B [Physics Department and Institute for Pure and Applied Physical Sciences, La Jolla, CA (United States)

    1998-06-01

    We have investigated the damage induced by irradiation in Y{sub 1}Ba{sub 2}Cu{sub 3}O{sub 7-x} silver added samples. The samples were prepared with 0 and 6.5 wt% of silver and irradiated by high-energy {gamma} irradiation (50-150 Mrad). The roles of silver and dosage irradiation are discussed in terms of their effects on microstructure, crystallinity, critical temperature (T{sub c}) and zero-resistance temperature (T{sub 0}). After irradiation, T{sub c} decreased while the room-temperature electrical resistance increased by a factor of 8 for some of the samples. The difference in T{sub 0} between irradiated and non-irradiated YBCO samples was of the order of 10 K. We have found that the difference is bigger for silver-added samples. We have also observed several changes in diffraction patterns of YBCO and YBCO-silver samples. SEM images, EDS and XPS analysis showed that silver resided inside the grains as single atoms and as metallic clusters. The relative concentrations of the elements in samples were quantified by Auger electron spectroscopy. The values showed a gradual increase for radiation doses ranging between 0 and 100 Mrad. For doses up to 100 Mrad, J{sub c} decreased because of the weak-link breakage induced by high doses of {gamma} rays. (author)

  19. Sorption of {sup 60} Co on inorganic solids; Sorcion de {sup 60} Co en solidos inorganicos

    Energy Technology Data Exchange (ETDEWEB)

    Granados C, F.; Bulbulian G, S. [ININ, 52045 Ocoyoacac, Estado de Mexico (Mexico); Mardel V, B. [UAM-I, 09340 Mexico D.F. (Mexico)

    2003-07-01

    The behavior of sorption of the {sup 60} Co in aqueous solution under static conditions to different values of pH of the aqueous solution (1, 3, 5, 7, and 10) on MgO, MnO{sub 2}, SnO, TiO{sub 2}, activated carbon and calcinate hydrotalcite was investigated. It was found that the best sorbents of the {sup 60} Co was the MnO{sub 2}, activated carbon and TiO{sub 2} whose sorption was incremented when increasing the pH value of the aqueous solutions, in the one case of the hydrated oxides, the {sup 60} Co interacted with the electrically charged surface of the sorbents that depends on the pH of the solution and of the point of zero charge (zpc) of the sorbent. (Author)

  20. Effects of gamma radiation (60Co) on the main physical and chemical properties of health care packaging and their compounds paper and multilayer plastic film, used for health products sterilization

    International Nuclear Information System (INIS)

    Porto, Karina Meschini Batista Geribello

    2013-01-01

    Gamma radiation is one of the technologies applied for the sterilization of packaging systems containing products for health. During sterilization process it is critical that the properties of packages are maintained. In this study two samples of commercial pouch packaging comprised of surgical grade paper on one side and the other side multilayer plastic film were irradiated with gamma rays. The following doses were applied 25 kGy (1,57 kGy/h) and 50 kGy (1,48 kGy/h). One packaging sample was paper formed by softwood fibers and multilayer plastic film based on poly(ethylene terephthalate) (PET)/polyethylene (PE). The second type of paper sample was made by a mixture of softwood and hardwood fibers and multilayer plastic film based on polyethylene terephthalate (ethylene) (PET)/polypropylene (PP). The effects of radiation on the physical and chemical properties of papers and multilayer plastic films, as well as the properties of the package were studied. The paper was the more radiation sensitive among the studied materials and radiation effects were more pronounced at brightness, pH, tearing resistance, bursting strength and tensile strength. Nonetheless, worst comparatively effects were noted on the sample made by a mixture of softwood and hardwood fibers. The porosity of paper was enhanced by 50 kGy. In the case of plastic films, radiation effects on tensile strength was the most pronounced property for both samples. In the case of the packaging the sealing resistance decreased with radiation. The effects observed for the treatment at 50 kGy were more pronounced when compared to 25 kGy. This last is the dose which is usually applied to sterilize health products. A dosimetry study was performed during irradiation at 25 kGy, 40 kGy and 50 kGy and its importance may be reported by the average dose variation 20 %. (author)

  1. Effect of 60Co radiation on peritoneal cells

    International Nuclear Information System (INIS)

    Villavicencio, A.L.C.H.; Mastro, N.L. del.

    1988-07-01

    This work deals with the effect of 60 Co gamma irradiation on the levels and quality of peritoneal cells of albino mice. The cells were obtained from peritoneal exudate, fixed and stained in 30% glacial acetic acid containing 0,5% cristal violet. Os exudates from irradiated and control animals the qualitative analysis and the counting of different cell populations were performed one hour, three days and six days after irradiation with 9 Gy. All the cell populations from the peritoneal exudate shown a decrease 3 days after the irradiation with 9.0. Gy but the different cellular populations dimished in unlike proportions. The data reaffirm the discrepance in radiosensivity of the diverse peritoneal cell populations. (author) [pt

  2. Seed germination of peanuts irradiated with cobalt (60CO)

    International Nuclear Information System (INIS)

    Alves, Niedja Marrize C.; Almeida, Francisco de Assis C.; Gomes, Josivanda P.; Pessoa, Elvira B.; Leal, Artur S. Cavalcanti

    2010-01-01

    This work was realized to evaluate the effect of gamma irradiation ( 60 Co) at doses 0, 0.50, 1.00, 1.50, 2.00, 2.50, 3.00 and 4.00 kGy, on germination of seeds of peanut, cultivar BR1. Irradiation Department of Nuclear, UFPE, where he received after the irradiation, they were stored in packing of PET and polyethylene braided with a time of 90 days. Through the results, obtained monthly, concluded that the dose of 0.5 kGy was effective in the germination of seeds of peanut, not affecting its power of germination and overcoming the witness within 30 to 60 days. (author)

  3. Dosimetry of a gammabeam 650 60Co irradiator

    International Nuclear Information System (INIS)

    Escobedo, J.F.; Nascimento Filho, V.F.; Barros Ferraz, E.S. de

    1980-01-01

    Fricke and glass dosimetries allowed the determination of some basic irradiation of the γ source Gamma-beam 650, 29,080 Ci of 60 Co on April 1st., 1974 made by Atomic Energy of Canada Limited, in operation at CENA - Piracicaba, Sa o Paulo, Brazil. The number of radioactive capsules in each of the 12 exposure tubes, the uniformity of the activity related to each tube and the mean position of the set of capsules during exposition were determined. The exposure rate at the central point of the internal cavity relatively to the diameter formed by 12 active tubes, were experimentally determined by Fricke dosimetry. The results agreed with those indicated in the operation manual of the irradiator source. (Author) [pt

  4. Perpendicularity among the linear polarization directions of {gamma} radiations coming from the positron-electron annihilation: entanglement or conservation law; Perpendicularidade entre as direcoes de polarizacao linear das radiacoes-{gamma} originarias do processo de aniquilacao positron-eletron: emaranhamento ou lei de conservacao

    Energy Technology Data Exchange (ETDEWEB)

    Cavalcante, Jose T.P.D.; Silva, Paulo R.J.; Saitovitch, Henrique [Centro Brasileiro de Pesquisas Fisicas (CBPF/MCT), Rio de Janeiro, RJ (Brazil). Lab. de Correlacao Angular

    2010-07-01

    The results of the experiments presented in this paper, concludes that the number of coincidences among the R{gamma} polarized by the EC-method is bigger with the detectors perpendicularly positioned (90 deg) than the parallel (0 deg). It can also observed that the R{gamma} source used presents, close to the 511 keV, another R{gamma} source with more problematic shielding

  5. The effect of the substitution of D{sub 2}O for H{sub 2}O on the degradation of myosin {beta} in solution by heat and by {sup 60}Co {gamma} radiation (1962); Effet de la substitution de D{sub 2}O a H{sub 2}O sur l'alteration de la Myosine B en solution par la chaleur et par les rayons {gamma} du {sup 60}CO (1962)

    Energy Technology Data Exchange (ETDEWEB)

    Pinset-Harstrom, I.; Fritsch, A. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1962-07-01

    (1) Alterations of myosin B produced by heat or irradiation are shown to be qualitatively identical as demonstrated by analytical centrifugation. (2) A considerable isotope effect was demonstrated using 75 per cent D{sub 2}O in the solvent. The sensitivity of myosin B to heat and irradiation is discussed in the light of this isotope effect. (3) Polymers appearing upon heat treatment of myosin B seem to be of a very different nature than the polymers occurring alter a similar treatment upon myosin A. Polymers obtained from myosin B can be depolymerized by ATP and they appear in a much narrower temperature range than myosin A polymers. This fact indicates a considerable difference in the activation enthalpies in the two reactions. (authors) [French] (1) Cette etude montre que les alterations de la myosine B provoquees par la chaleur et par l'irradiation aux rayons {gamma} sont - telles qu'elles apparaissent a l'ultracentrifugation analytique - qualitativement semblables. (2) Nous avons observe un effet isotopique considerable de la presence de 75 pour cent de D{sub 2}O dans le solvant sur la sensibilite de la myosine B envers ces deux agents, et nous avons presente une tentative d'explication de ce fait. (3) Les polymeres qui apparaissent apres un traitement par la chaleur de la myosine semblent etre d'une nature tres differente des polymeres que l'on voit apparaitre apres un traitement identique de la myosine A. Ceux obtenus a partir de le myosine B sont depolymerisables par l'intermediaire de l'ATP et apparaissent dans une zone de temperature beaucoup plus etroite que celles de la myosine A. Ce dernier fait indique une difference considerable de l'enthalpie d'activation des deux reactions. (auteurs)

  6. The effect of the substitution of D{sub 2}O for H{sub 2}O on the degradation of myosin {beta} in solution by heat and by {sup 60}Co {gamma} radiation (1962); Effet de la substitution de D{sub 2}O a H{sub 2}O sur l'alteration de la Myosine B en solution par la chaleur et par les rayons {gamma} du {sup 60}CO (1962)

    Energy Technology Data Exchange (ETDEWEB)

    Pinset-Harstrom, I; Fritsch, A [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1962-07-01

    (1) Alterations of myosin B produced by heat or irradiation are shown to be qualitatively identical as demonstrated by analytical centrifugation. (2) A considerable isotope effect was demonstrated using 75 per cent D{sub 2}O in the solvent. The sensitivity of myosin B to heat and irradiation is discussed in the light of this isotope effect. (3) Polymers appearing upon heat treatment of myosin B seem to be of a very different nature than the polymers occurring alter a similar treatment upon myosin A. Polymers obtained from myosin B can be depolymerized by ATP and they appear in a much narrower temperature range than myosin A polymers. This fact indicates a considerable difference in the activation enthalpies in the two reactions. (authors) [French] (1) Cette etude montre que les alterations de la myosine B provoquees par la chaleur et par l'irradiation aux rayons {gamma} sont - telles qu'elles apparaissent a l'ultracentrifugation analytique - qualitativement semblables. (2) Nous avons observe un effet isotopique considerable de la presence de 75 pour cent de D{sub 2}O dans le solvant sur la sensibilite de la myosine B envers ces deux agents, et nous avons presente une tentative d'explication de ce fait. (3) Les polymeres qui apparaissent apres un traitement par la chaleur de la myosine semblent etre d'une nature tres differente des polymeres que l'on voit apparaitre apres un traitement identique de la myosine A. Ceux obtenus a partir de le myosine B sont depolymerisables par l'intermediaire de l'ATP et apparaissent dans une zone de temperature beaucoup plus etroite que celles de la myosine A. Ce dernier fait indique une difference considerable de l'enthalpie d'activation des deux reactions. (auteurs)

  7. Another 60Co hot cell accident

    International Nuclear Information System (INIS)

    Steidley, K.D.; Zeik, G.S.; Ouellette, R.

    1979-01-01

    An accident at a commercial irradiation facility is described. Because of inadequate safety arrangements a worker entered the irradiation cell while the 500,000 Ci 60 Co source was exposed. The duration of the exposure was established by re-enactment of the worker's movements, and dose rates at various locations at the accident site were measured. From the results a total absorbed dose of 210 rad to the surface of the victim was calculated. This and results of chromosomal aberration studies (200 rad) and the worker's film badge reading (126 rad) lead to the assignment of a nominal 200 rad absorbed dose. The worker was taken to two local hospital emergency departments, both of which examined and discharged him. Eventually he was admitted to a medical centre with experience of radiation effects. His symptoms and treatment during his 7 week stay in hospital are described and his blood counts vs time are shown graphically. As a result of the unsatisfactory response of the emergency departments the NRC has modified its licensing procedures to include specific arrangements with a local hospital. It is stressed that accident dosage estimates should always be supplied to the patient or his personal physician. (author)

  8. Development and evaluation of a technique for in vivo monitoring of 60Co in human liver

    Science.gov (United States)

    Gomes, GH; Silva, MC; Mello, JQ; Dantas, ALA; Dantas, BM

    2018-03-01

    60Co is an artificial radioactive metal produced by activation of iron with neutrons. It decays by beta particles and gamma radiation and represents a risk of internal exposure of workers involved in the maintenance of nuclear power reactors. Intakes can be quantified through in vivo monitoring. This work describes the development of a technique for the quantification of 60Co in human liver. The sensitivity of the method is evaluated based on the minimum detectable effective doses. The results allow to state that the technique is suitable either for monitoring of occupational exposures or evaluation of accidental intakes.

  9. Deep underground measurements of 60Co in steel exposed to the Hiroshima atomic bomb explosion.

    Science.gov (United States)

    Hult, Mikael; Gasparro, Joël; Vasselli, Roberto; Shizuma, Kiyoshi; Hoshi, Masaharu; Arnold, Dirk; Neumaier, Stefan

    2004-01-01

    When using gamma-ray spectrometry performed deep underground, it is possible to measure 60Co activities down to 0.1 mBq in steel samples of some 100 g without any pre-concentration. It is thus still possible to measure 60Co induced by neutrons from the atomic bomb explosion in Hiroshima in pieces of steel collected at distances up to about 1200 m slant range. The results of non-destructive measurements of eight steel samples are compared with the 1986 Dose Re-Evaluation (DS86) model calculations.

  10. Deep underground measurements of 60Co in steel exposed to the Hiroshima atomic bomb explosion

    International Nuclear Information System (INIS)

    Hult, Mikael; Gasparro, J.Joeel; Vasselli, Roberto; Shizuma, Kiyoshi; Hoshi, Masaharu; Arnold, Dirk; Neumaier, Stefan

    2004-01-01

    When using gamma-ray spectrometry performed deep underground, it is possible to measure 60 Co activities down to 0.1 mBq in steel samples of some 100 g without any pre-concentration. It is thus still possible to measure 60 Co induced by neutrons from the atomic bomb explosion in Hiroshima in pieces of steel collected at distances up to about 1200 m slant range. The results of non-destructive measurements of eight steel samples are compared with the 1986 Dose Re-Evaluation (DS86) model calculations

  11. Deep underground measurements of {sup 60}Co in steel exposed to the Hiroshima atomic bomb explosion

    Energy Technology Data Exchange (ETDEWEB)

    Hult, Mikael E-mail: mikael.hult@cec.eu.int; Gasparro, J.Joeel; Vasselli, Roberto; Shizuma, Kiyoshi; Hoshi, Masaharu; Arnold, Dirk; Neumaier, Stefan

    2004-09-01

    When using gamma-ray spectrometry performed deep underground, it is possible to measure {sup 60}Co activities down to 0.1 mBq in steel samples of some 100 g without any pre-concentration. It is thus still possible to measure {sup 60}Co induced by neutrons from the atomic bomb explosion in Hiroshima in pieces of steel collected at distances up to about 1200 m slant range. The results of non-destructive measurements of eight steel samples are compared with the 1986 Dose Re-Evaluation (DS86) model calculations.

  12. Study on adsorption of 60Co in soils and minerals and transportation of 60Co in bean-soil system

    International Nuclear Information System (INIS)

    Feng Yonghong; Chen Chuanqun; Wang Shouxiang; Zhang Yongxi; Sun Zhiming

    1998-02-01

    The adsorption and desorption of 60 Co in soils and minerals, and the transportation, accumulation, distribution in bean-soil system are studied. The results are as follows: (1) 60 Co was adsorbed rapidly and desorbed difficultly by soils and minerals. The order of the saturated adsorption rate and K d (distribution coefficient) of 60 Co at the balance value was: kieselguhr>paddy soil (loamy clay)>yellowish red soil>kaoline>perlite>silt-loamy soil. The order of D f (desorption factor) value was: yellowish red soil>silt-loamy soil>kaoline>perlite>paddy soil (loamy clay)>kieselguhr. The dynamic behavior of 60 Co in the soils and minerals could be described as a closed two--compartment model. (2) After 60 Co was introduced to the bean-soil system, the concentration of 60 Co in the root is about 10.4∼23.3 times of that in the stalk, and 30 times of that in the bean pod. The negative correlation between the concentration of 60 Co in the soil and depth was detected, over 90 per cent of 60 Co was retained within 6 centimeters of the surface layer, the half residual depth was 2 centimeters. An opened two-compartment model was applied to describe the behavior of 60 Co in the bean-soil system

  13. The application of PLC in 60Co container inspection system

    International Nuclear Information System (INIS)

    Huang Yibin; Xiang Xincheng

    2001-01-01

    The author discusses the interlock technique of 60 Co container inspection system, and introduces the hardware structure and program of interlock control system using PLC. Due to adopting PLC distributed control, the system works stably and reliably. The successful application of PLC in 60 Co container inspection system has some use for reference in nuclear technology field

  14. Transfer of 60Co from midwater squid to sperm whales

    International Nuclear Information System (INIS)

    Umezu, Takeshi; Minamisako, Yoko; Ebihara, Hiroshi; Watanabe, Hiroshi.

    1984-01-01

    Sperm whales are notable squid-eaters. They feed mainly on medium to large-sized cephalopods at midwater levels and defecate near the surface. This suggests the existence of an upward transport of 60 Co by sperm whales from the mesopelagic zone (150-1,200m). To elucidate this squid-whale route for this artificial radionuclide, 60 Co content was determined in squid and in predator whales captured by commercial whaling. In the Cephalopoda livers 60 Co levels of 30-500 mBq kg -1 wet were found and in the viscera of Odontoceti (toothed whales) 15-40 mBq kg -1 wet. About 0.3% of 60 Co ingested was estimated to be retained in a 23-year-old male sperm whale. In the livers of Bryde's whales, 60 Co levels of 40-80 mBq kg -1 wet were detected, but not in euphausiids and sardines, their possible prey. The level of Co in sperm whales was nearly the same as in Bryde's whales. Specific radioactivity 60 Co/ 59 Co in mBq μg -1 was several times higher in sperm whale (1.1-1.6) than in cephalopods (0.19-0.77). Eating prey with a high content of 60 Co in the 1960's may have contributed to the present body burden in sperm whales with a long-life span. However, the origin of 60 Co in Bryde's whales is unknown. (author)

  15. Effect of 60Co radiation processing in mate (Ilex paraguariensis)

    International Nuclear Information System (INIS)

    Furgeri, Camilo

    2009-01-01

    The mate (Ilex paraguariensis), a native species from South America, is mainly consumed as typical beverage called chimarrao and terere. An important problem that has been afflicting this product since a long time is its natural fungal contamination responsible to affect its physical, health and nutritional qualities. In order to improve this product quality, radiation processing can be effective in reducing pathogens levels, with minimal nutritional and sensory changes. The aim of this study was to evaluate the effectiveness of gamma radiation from 60 Co at doses 0, 3, 5, 7 and 10 kGy in reducing fungal contamination in mate, as well as analyze its nutritional and sensory characteristics. The following methodologies were applied: analysis of yeast and mold, total phenolic compounds analysis, antioxidant analysis, quantification of phenolic compounds and xanthines by liquid chromatography and sensory analysis. Microbiological analysis showed a decreasing molds and yeasts growth with increasing radiation doses. Regardless of the radiation dose applied there were no decrease of total phenolic compounds in both infusions. Chimarrao samples irradiated with 7 and 10 kGy showed a decrease in the DPPH radical-scavenger activity, nevertheless for terere samples, there were no significant difference. Chimarrao chromatographic profile did not show a variation on xanthines quantification, however a 10 kGy radiation dose caused a change to phenolic compounds quantitative profile. Terere samples did not show any significant difference to any analyzed compounds. Sensory analysis did not exhibit a significant difference between irradiated and non irradiated chimarrao samples, as well as between irradiated and non irradiated terere samples. It could be concluded that gamma radiation processing of mate may be a feasible alternative to industry, since there was a reduction on fungal contamination, without changes in sensory qualities and with minimum alterations in quantitative and

  16. Effect of 60CO radiation processing in mate (Ilex paraguariensis)

    International Nuclear Information System (INIS)

    Furgeri, Camilo

    2009-01-01

    The mate (Ilex paraguariensis), a native species from South America, is mainly consumed as typical beverage called chimarrao and terere. An important problem that has been afflicting this product since a long time is its natural fungal contamination responsible to affect its physical, health and nutritional qualities. In order to improve this product quality, radiation processing can be effective in reducing pathogens levels, with minimal nutritional and sensory changes. The aim of this study was to evaluate the effectiveness of gamma radiation from 60 Co at doses 0, 3, 5, 7 and 10kGy in reducing fungal contamination in mate, as well as analyze its nutritional and sensory characteristics. The following methodologies were applied: analysis of yeast and mold, total phenolic compounds analysis, antioxidant analysis, quantification of phenolic compounds and xanthines by liquid chromatography and sensory analysis. Microbiological analysis showed a decreasing molds and yeasts growth with increasing radiation doses. Regardless of the radiation dose applied there were no decrease of total phenolic compounds in both infusions. Chimarrao samples irradiated with 7 and 10kGy showed a decrease in the DPPH radical-scavenger activity, nevertheless for terere samples, there were no significant difference. Chimarrao chromatographic profile did not show a variation on xanthines quantification, however a 10kGy radiation dose caused a change to phenolic compounds quantitative profile. Terere samples did not show any significant difference to any analyzed compounds. Sensory analysis did not exhibit a significant difference between irradiated and non irradiated chimarrao samples, as well as between irradiated and non irradiated terere samples. It could be concluded that gamma radiation processing of mate may be a feasible alternative to industry, since there was a reduction on fungal contamination, without changes in sensory qualities and with minimum alterations in quantitative and

  17. Behavior of the sorption of {sup 60} Co in aqueous solution on inorganic materials as function of p H; Comportamiento de la sorcion del {sup 60} Co en solucion acuosa sobre materiales inorganicos como una funcion del pH

    Energy Technology Data Exchange (ETDEWEB)

    Granados, F.; Bulbulian, S.; Solache R, M. [ININ, 52045 Ocoyoacac, Estado de Mexico (Mexico); Bertin, V. [UAM-I, 09340 Mexico D.F. (Mexico)

    2004-07-01

    The sorption of the {sup 60} Co is evaluated in aqueous solution on Mg O, MnO{sub 2}, TiO{sub 2}, Sn O, activated carbon and hydrotalcite calcined as a function of the p H, using the method for lots and quantifying at the {sup 60} Co for gamma spectrometry. Likewise it was explained the one behavior of the sorption of the {sup 60} Co in the materials with base in the chemical species of this radioactive isotope in aqueous solution. The chemical species of the {sup 60} Co in solution were identified by electrophoresis of high voltage for the different p H values. It was found that under the experimental conditions, the {sup 60} Co showed a significant sorption on MnO{sup 2}, TiO{sup 2} and activated carbon. On the other hand, in Mg O, Sn O and calcined hydrotalcite also was observed a sorption, although in smaller quantities. The studied hydrated metallic oxides retained the {sup 60} Co for ion exchange via. It was found that the {sup 60} Co was present as a cationic specie to p H 1, 3, 5 and 7 and like a neutral specie to alkaline p H. (Author)

  18. National comparison of activity measurements of 131I, 60Co, and 133Ba in Indonesia

    International Nuclear Information System (INIS)

    Wurdiyanto, Gatot; Marsoem, Pujadi; Widodo, Susilo; Iskandar, Dadong; Muhayatun; Hartoyo, Unggul; Sugino; Gede Sutresna, I.; Hutabarat, Tommy; Suparman, Ibon; Purwanto, Setyo

    2014-01-01

    National comparisons of radioactivity measurements of 131 I and 60 Co (in 2010) and of 133 Ba (in 2011) were carried out within the framework of the National Radiation Metrology Laboratory Program of Indonesia, coordinated by PTKMR-BATAN. Eleven laboratories took part in the comparison, and all measurements were made using gamma spectrometry, on point sources with an activity between 2000 Bq and 6000 Bq. Several laboratories reported values which were more than 10% different from the reference value for 131 I and 133 Ba. Possible reasons for these differences are discussed. This program will be continued with other radionuclides to maintain and control quality assurance for the local laboratories. - Highlights: • PTKMR-BATAN coordinated national comparison of 131 I, 60 Co and 133 Ba. • Eleven laboratories took part in the comparison. • All measurements were done using gamma spectrometry methods. • For 131 I and 133 Ba, 3 laboratories have more than 10% difference from reference laboratory value

  19. Behavior of the sorption of 60 Co in aqueous solution on inorganic materials as function of p H

    International Nuclear Information System (INIS)

    Granados, F.; Bulbulian, S.; Solache R, M.; Bertin, V.

    2004-01-01

    The sorption of the 60 Co is evaluated in aqueous solution on Mg O, MnO 2 , TiO 2 , Sn O, activated carbon and hydrotalcite calcined as a function of the p H, using the method for lots and quantifying at the 60 Co for gamma spectrometry. Likewise it was explained the one behavior of the sorption of the 60 Co in the materials with base in the chemical species of this radioactive isotope in aqueous solution. The chemical species of the 60 Co in solution were identified by electrophoresis of high voltage for the different p H values. It was found that under the experimental conditions, the 60 Co showed a significant sorption on MnO 2 , TiO 2 and activated carbon. On the other hand, in Mg O, Sn O and calcined hydrotalcite also was observed a sorption, although in smaller quantities. The studied hydrated metallic oxides retained the 60 Co for ion exchange via. It was found that the 60 Co was present as a cationic specie to p H 1, 3, 5 and 7 and like a neutral specie to alkaline p H. (Author)

  20. Safety protection and technical improvement of 60Co irradiation facilities

    International Nuclear Information System (INIS)

    Zhou Yongxing; Liang Cannan

    1993-01-01

    To ensure personal safety, some improvements has been made in the design of 60 Co irradiation compartment. The shielding door was interlocked while the 60 Co source to be lifted to the irradiation position or lowered to the shielded position. A universal change-over switch was used to cut the power supply when the source moved beyond the limits. Both γ-ray alarm and a closed-TV system were adopted. The electromagnetic attraction method was employed to shift the 60 Co source from the Pb container to the source pipe

  1. Measurements of 60Co in spoons activated by neutrons during the JCO criticality accident at Tokai-mura in 1999.

    Science.gov (United States)

    Gasparro, J; Hult, M; Komura, K; Arnold, D; Holmes, L; Johnston, P N; Laubenstein, M; Neumaier, S; Reyss, J-L; Schillebeeckx, P; Tagziria, H; Van Britsom, G; Vasselli, R

    2004-01-01

    Neutron activated items from the vicinity of the place where the JCO criticality accident occurred have been used to determine the fluence of neutrons around the facility and in nearby residential areas. By using underground laboratories for measuring the activation products, it is possible to extend the study to also cover radionuclides with very low activities from long-lived radionuclides. The present study describes gamma-ray spectrometry measurements undertaken in a range of underground laboratories for the purpose of measuring (60)Co more than 2 years after the criticality event. The measurements show that neutron fluence determined from (60)Co activity is in agreement with previous measurements using the short-lived radionuclides (51)Cr and (59)Fe. Limits on contamination of the samples with (60)Co are evaluated and shown to not greatly affect the utility of neutron fluence determinations using (60)Co activation.

  2. The radiation protection environmental assesment for 60Co irradiation room

    International Nuclear Information System (INIS)

    Zheng Meiyang; Jin Guohua; Shen Genfang

    2010-01-01

    60 Co source is applied in the process such as sterilizing agricultural products in irradiation room of some Academy of Agricultural Sciences, which is very effective in agricultural applications. However, 60 Co is highly toxic, once the leak, the consequences would be disastrous. So it is necessary to summarize the radiation protection and safety evaluation of the irradiation room indoor and outdoor, to ensure the health and lives of the staff and the surrounding population. The radiation detectors monitor six points around the irradiation room. Results show that design of irradiation room of Academy of Agricultural Sciences are mostly safe and reliable, regardless of the source in working condition. And consequences also show 60 Co source in the normal operating will not put adverse effects on the surrounding environment. In addition, the outer radiation protective measures are also outlined, in view of 60 Co own identity. (authors)

  3. Soil properties related to 60Co bioavailability in tropical soils

    International Nuclear Information System (INIS)

    Bartoly, Flavia; Wasserman, Maria Angelica; Rochedo, Elaine Ruas Rodriguez; Viana, Aline Gonzalez; Souza, Rodrigo Camara; Oliveira, Giselle Rodrigues; Reis, Wagner Goncalves Soares; Perez, Daniel Vidal

    2005-01-01

    This work presents the results of field experiments to obtain soil to plants Transfer factor (TF) for 60 Co in reference plants cultivated in Ferralsol, Acrisol and Nitisol. These soils represent the majority of Brazilian agricultural area. Values of TF varied from 0.001 to 0.05 for corn and from 0.001 to 0.81 for cabbage. Results of 60 Co TF were discussed in relation to the physical and chemical properties of the soils and 60 Co geochemical partition. The sequential chemical extraction showed that more than 40% of the 60 Co present in the soils are associated to manganese oxides. These results will provide regional values for parameters used in the environmental radiological modeling aiming to optimize the planning of emergency interventions or the waste management related to tropical soils. (author)

  4. 60Co retention by a freshwater planktonic alga Scenedesmus obliquus

    International Nuclear Information System (INIS)

    Nucho, R.; Baudin, J.P.

    1989-01-01

    This paper presents the results of various decontamination experiments aimed at evaluating 60 Co retention by Scenedesmus obliquus, and the respective role played by absorption and adsorption in the contamination of the alga by this radionuclide. The physiological condition of cells is not involved in radiocobalt desorption, which seems to indicate that the phenomenon is of a passive nature. When the precontamination time is extended, the proportion of adsorbed 60 Co decreases, and the final percentage of radionuclide retained increases. These results are confirmed by bringing cells into contact with a strong chelating agent. The elimination of 60 Co in the presence of EDTA undergoes a sharp decrease which is correlated with the increase in the duration of the precontamination phase. The use of a weaker complexing agent, such as NaCl, leads to the hypothesis of there being 2 types of 60 Co receptor on cell membranes. (author)

  5. Distribution of 60Co and 54Mn in graphite material of irradiated HTGR fuel assemblies

    International Nuclear Information System (INIS)

    Hayashi, Kimio; Kikuchi, Teruo; Kobayashi, Fumiaki; Minato, Kazuo; Fukuda, Kousaku; Ikawa, Katsuichi; Iwamoto, Kazumi

    1984-05-01

    Distribution of 60 Co and 54 Mn was measured in the graphite sleeves and blocks of the third and fourth HTGR fuel assemblies irradiated in the Oarai Gas Loop-1 (OGL-1), which is a high temperature inpile gas loop installed in the Japan Materials Testing Reactor (JMTR) of Japan Atomic Energy Research Institute (JAERI). Axial and circumferential profiles were obtained by gamma spectrometry, and radial profiles by lathe sectioning with gamma spectrometry. Distribution of 60 Co is in good agreement with that of thermal neutron flux, and the Co content in the graphite is estimated to be -- 1 x 10 -9 in weight fraction. Concentration of 54 Mn decreases toward the axial center in its axial profile, and radially is almost uniform inside and appreciably higher at free surfaces. An estimated Fe content of --10 -8 in wight fraction is smaller by two orders of magnitude than that from chemical analysis. Higher concentraion of 60 Co and 54 Mn at the free surfaces suggests the importance of transportation process of these nuclides in the coolant loop. (author)

  6. Experimental study on the temperature conditions for rod and plane irradiators with 60Co source

    International Nuclear Information System (INIS)

    Stepanov, G.D.; Osipov, V.B.; Sarapkin, I.I.; Chizhikov, V.A.

    1977-01-01

    The formation of a temperature field of rod and flat 60 Co irradiators has been studied. The experiments are carried out on a gamma installation. It has been shown that for a stationary operating mode the maximum cassette temperature (when the cassette contains a 60 Co source) is 148 deg C at maximum permissible temperature of 250 deg C. When ampoules containing the sources with maximum activity (640 Ci) are loaded into cassettes they have the temperature of 184 deg C. The reciprocal screening influence of rod irradiators gives the temperature rise of 8-10 deg in each element. The irradiators under study reach a stationary thermal operating mode in 150 min after the sources are elevated to the operating position

  7. Bioaccumulation of 137Cs and 60Co by Helianthus annuus

    International Nuclear Information System (INIS)

    Hornik, M.; Pipiska, M.; Vrtoch, L.; Augustin, J.; Lesny, J.

    2005-01-01

    The 60 Co and 137 Cs bioaccumulation by Helianthus annuus L. was measured during 9 day cultivation at 20 ± 2 o C in hydroponic Hoagland medium. Previous starvation for K + and for NH 4 + 2.2 and 2.7 times, respectively, enhanced 137 Cs uptake rate. Previous cultivation in surplus of K + ions 50 mmol·l -1 has no effect on 137 Cs bioaccumulation rate. Both 137 Cs and 60 Co bioaccumulation significantly increase with dilution of basic Hoagland medium up to 1:7 for caesium and up to 1:3 for cobalt followed by mild decrease at higher dilutions. Root to shoot specific 137 Cs radioactivity ratio (Bq.g -1 /Bq·g -1 , fresh wt.) increased with dilution from 1.46 to 9.6-9.8. The values root to shoot specific radioactivity ratio for 60 Co were less dependent on the nutrient concentrations and were within the range 5.7 to 8.5. 137 Cs was localized mainly in young leaves (30%) and roots (39%) and 60 Co mainly in roots (67%) and leaves (20%). Obtained data showed less sensitivity of 60 Co uptake by sunflower on nutrient concentration in hydroponic media. (author)

  8. Concentration of 60Co by marine organisms through sediments

    International Nuclear Information System (INIS)

    Nakahara, Motokazu; Koyanagi, Taku; Saiki, Masamichi

    1976-01-01

    Uptake of 60 Co absorbed on sea sands by benthic marine organisms was observed in laboratory experiments, since the radioactive cobalt released from nuclear power plants or other establishments into coastal seawater trends to be absorbed on sea sediments and also various kinds of marine organisms live in bottom sediments. Few kinds of flatfishes (Limanda spp.) and shrimp (Trachypenaeus curvirostris) were reared in aquariums contained seawater and sea sands which were highly contaminated with 60 Co previously, and whole body retention and distribution of radioactivity were measured on the organisms taken up from the aquariums occasionally by a scintillation counter. Uptake of 60 Co from ingested sea sands was also observed on the flatfishes administrating the contaminated sands orally. Concentration of 60 Co by the flatfishes reared in the sands was not significant while the shrimp showed high retention of the radioactivity. The food habit of shrimp which usually feeds on organic detritus with other small benthic organisms is different from that of flatfishes, one of the carnivorous, and considered to bring the difference on the pathway of radionuclides concentration. Assimilation of 60 Co via the digestive tract of flatfishes through the sands was estimated as about 10 per cent of the administrated radioactivity. (auth.)

  9. Measurements of lattice and grain boundary diffusivities of 60Co and 54Mn in type 316 stainless steel

    International Nuclear Information System (INIS)

    Polley, M.V.

    1981-02-01

    Diffusion in type 316 stainless steel was studied by depositing 60 Co and 54 Mn on flat polished surfaces and heating. Diffusion profiles, obtained after gamma-counting slices removed by hand grinding, were analysed using Suzuoka's ''instantaneous source'' model. (author)

  10. Monte Carlo Dosimetry of the 60Co BEBIG High Dose Rate for Brachytherapy.

    Directory of Open Access Journals (Sweden)

    Luciana Tourinho Campos

    Full Text Available The use of high-dose-rate brachytherapy is currently a widespread practice worldwide. The most common isotope source is 192Ir, but 60Co is also becoming available for HDR. One of main advantages of 60Co compared to 192Ir is the economic and practical benefit because of its longer half-live, which is 5.27 years. Recently, Eckert & Ziegler BEBIG, Germany, introduced a new afterloading brachytherapy machine (MultiSource®; it has the option to use either the 60Co or 192Ir HDR source. The source for the Monte Carlo calculations is the new 60Co source (model Co0.A86, which is referred to as the new BEBIG 60Co HDR source and is a modified version of the 60Co source (model GK60M21, which is also from BEBIG.The purpose of this work is to obtain the dosimetry parameters in accordance with the AAPM TG-43U1 formalism with Monte Carlo calculations regarding the BEBIG 60Co high-dose-rate brachytherapy to investigate the required treatment-planning parameters. The geometric design and material details of the source was provided by the manufacturer and was used to define the Monte Carlo geometry. To validate the source geometry, a few dosimetry parameters had to be calculated according to the AAPM TG-43U1 formalism. The dosimetry studies included the calculation of the air kerma strength Sk, collision kerma in water along the transverse axis with an unbounded phantom, dose rate constant and radial dose function. The Monte Carlo code system that was used was EGSnrc with a new cavity code, which is a part of EGS++ that allows calculating the radial dose function around the source. The spectrum to simulate 60Co was composed of two photon energies, 1.17 and 1.33 MeV. Only the gamma part of the spectrum was used; the contribution of the electrons to the dose is negligible because of the full absorption by the stainless-steel wall around the metallic 60Co. The XCOM photon cross-section library was used in subsequent simulations, and the photoelectric effect, pair

  11. 60Co γ-ray attenuation coefficient of barite concrete

    International Nuclear Information System (INIS)

    Bouzarjomehri, F.; Bayat, T.; Dashti, M. H.; Ghisari, J.; Abdoli, N.

    2006-01-01

    Recently, the use of medium and high energy X-rays has increased in Iran, and radiotherapy centers along with a variety of accelerators have been installed in some provinces. Hence, there is not sufficient skill in designing and installing radiotherapy treatment rooms. This study was conducted to evaluate the efficacy of different mixtures of barite concrete for shielding the radiotherapy rooms. This way, we have emphasized on determining the size and amount of barite aggregations to achieve the maximum radiation attenuation which leads to minimizing wall thickness in treatment room. Materials and Methods: To increase concrete density, the barite aggregation was added to concrete. Different size variations of barite aggregates mixed with different water/cement ratio were examined. The dimension of cubic concrete specimens for compression strength test was 15*15*15 cm. The rectangular barite concrete blocks with different compressions as used for strength test with cross section of 10*10 cm, and thicknesses from 5 to 40 cm were used for radiation attenuation test. To do so, concrete specimens were irradiated by gamma beam of 60 Co (Phoenix Theratron). The transmission radiation through the blocks was measured by a Farmer ionization chamber (Fc 65 P). Results: Our findings showed that in all specimens the highest mean compression strength was related to the specimens with equal ratio of fine to coarse barite aggregates, but the lowest half value layer was obtained from mixtures with fine to coarse ratio of 35/65. The concrete sample with a 0.45 water/cement ratio, 350 kg/m3 cement and equal amounts of fine and coarse barite sands had nearly minimum half value layer (half value layer), and maximum compression strength, so the sample was considered as the best barite concrete sample. Conclusion: Since half value layer of the barite concrete specimens with the same compression strength is markedly lower than the conventional concrete, and that there are quite a number

  12. Experimental study of half-life determinations using 60Co

    International Nuclear Information System (INIS)

    Rutledge, A.R.; Smith, L.V.; Merritt, J.S.

    1983-01-01

    The half-life of 60 Co was determined by two methods, (i) the 4π#betta# ionization chamber method and (ii) the 4π #betta#-#betta# coincidence method. The first is a relative counting method in which the only rate-dependent correction is for background, whereas the second is an 'absolute' counting method which involves several rate-dependent corrections. In addition, various methods of calculation of the results were tested. The half-life values for the two counting systems were in good agreement and the weighted mean value for the half-life of 60 Co was found to be 1925.02+-0.47 d. (orig.)

  13. Information managing in 60Co container inspection system

    International Nuclear Information System (INIS)

    Wu Zhifang; Gu Bohua; Zhou Liye; An Jigang; Liu Yisi

    1998-01-01

    The design, maintenance and realization of information managing database in 60 Co container inspection system made by INET of Tsinghua University is introduced. The technique of Open Database Connectivity (ODBC) is adopted to develop a general format database including text and graphic information. The database application is developed with Visual C ++ 5.0 programming language to run in 32-bit Windows operation system. It conforms to Client/Server model and supports network communication. It works very well in the laboratory emulator of 60 Co container inspection system

  14. /sup 60/Co accumulation by freshwater plants under natural conditions

    Energy Technology Data Exchange (ETDEWEB)

    Trapeznikov, A V; Trapeznikova, V N [AN SSSR, Sverdlovsk. Inst. Ehkologii Rastenij i Zhivotnykh

    1979-03-01

    In search for effective bioindicators of radioactive contamination of cooler-reservoirs in atomic power plants the accumulation of /sup 60/Co by four species of higher aquatic plants most widely distributed in the Urals was studied, namely by Elodea canadensis, Ceratophyllum demersum, Lemna minor and Potamogeton pectinatus. It is shown that such plants as Ceratophyllum demersum and Elodea canadensis having the accumulation coefficients of /sup 60/Co 33,500 and 21,500, respectively, can be recommended as bioindicators of this radionuclide in reservoirs contaminated with radioactive cobalt.

  15. On 60Co accumulation by freshwater plants under natural conditions

    International Nuclear Information System (INIS)

    Trapeznikov, A.V.; Trapeznikova, V.N.

    1979-01-01

    In search for effective bioindicators of radioactive contamination of cooler-reservoirs in atomic power plants the accumulation of 60 Co by four species of higher aquatic plants most widely distributed in the Urals was studied, namely by Elodea canadensis, Ceratophyllum demersum, Lemna minor and Potamogeton pectinatus. It is shown that such plants as Ceratophyllum demersum and Elodea canadensis having the accumulation coefficients of 60 Co 33,500 and 21,500, respectively, can be recommended as bioindicators of this radionuclide in reservoirs contaminated with radioactive cobalt

  16. Decontamination of fermented chicken feet by 60Co irradiation

    International Nuclear Information System (INIS)

    Gao Peng; Wang Yan; Huang Min; Wu Ling; Du Xiaoying; Lei Qing; Xie Yan

    2010-01-01

    Fermented chicken feet was treated by 60Co irradiation, and the aerobic plate count, enumeration of coliforms, pathogens and TBARS value were measured during storage. The results showed that, aerobic plate count of all irradiated samples was lower than control, and enumeration of coliforms, and pathogens of Staphylococcus aureus, Shigella, Salmonella were not detected. TBARS value of all treatments was stable during 60 d storage. It could be concluded that 60Co irradiation of chicken feet was an effective method to prolong its shelf life. (authors)

  17. Dosimetric evaluation of Radiotherapy units wit 60Co

    International Nuclear Information System (INIS)

    Leon, B. Salinas de; Tovar M, V.; Becerril V, A.

    2000-01-01

    The SSDL network of the IAEA performs, every year, quality audit tests for radiotherapy services ( 60 Co units and linear accelerators), and for national SSDL as well. Because of the SSDL-Mexico results in these tests and due to our enthusiasm and confidence in our work, a parallel test has been done , which is described in this talk as well as the results. Nowadays, a second parallel test goes up, which could confirm our optimism and open the possibility to our country to start a national dosimetric audit of 60 Co radiotherapy units. (Author)

  18. Comparative analysis of 60Co intensity-modulated radiation therapy

    International Nuclear Information System (INIS)

    Fox, Christopher; Romeijn, H Edwin; Lynch, Bart; Dempsey, James F; Men, Chunhua; Aleman, Dionne M

    2008-01-01

    In this study, we perform a scientific comparative analysis of using 60 Co beams in intensity-modulated radiation therapy (IMRT). In particular, we evaluate the treatment plan quality obtained with (i) 6 MV, 18 MV and 60 Co IMRT; (ii) different numbers of static multileaf collimator (MLC) delivered 60 Co beams and (iii) a helical tomotherapy 60 Co beam geometry. We employ a convex fluence map optimization (FMO) model, which allows for the comparison of plan quality between different beam energies and configurations for a given case. A total of 25 clinical patient cases that each contain volumetric CT studies, primary and secondary delineated targets, and contoured structures were studied: 5 head-and-neck (H and N), 5 prostate, 5 central nervous system (CNS), 5 breast and 5 lung cases. The DICOM plan data were anonymized and exported to the University of Florida optimized radiation therapy (UFORT) treatment planning system. The FMO problem was solved for each case for 5-71 equidistant beams as well as a helical geometry for H and N, prostate, CNS and lung cases, and for 3-7 equidistant beams in the upper hemisphere for breast cases, all with 6 MV, 18 MV and 60 Co dose models. In all cases, 95% of the target volumes received at least the prescribed dose with clinical sparing criteria for critical organs being met for all structures that were not wholly or partially contained within the target volume. Improvements in critical organ sparing were found with an increasing number of equidistant 60 Co beams, yet were marginal above 9 beams for H and N, prostate, CNS and lung. Breast cases produced similar plans for 3-7 beams. A helical 60 Co beam geometry achieved similar plan quality as static plans with 11 equidistant 60 Co beams. Furthermore, 18 MV plans were initially found not to provide the same target coverage as 6 MV and 60 Co plans; however, adjusting the trade-offs in the optimization model allowed equivalent target coverage for 18 MV. For plans with comparable

  19. Development and evaluation of a technique for in vivo monitoring of {sup 60}Co in human liver

    Energy Technology Data Exchange (ETDEWEB)

    Dantas, B.M.; Dantas, A.L.A.; Gomes, G.H.; Silva, M.C.; Mello, J.Q., E-mail: bmdantas@ird.gov.br [Instituto de Radioproteção e Dosimetria (IRD/CNEN-RJ), Rio de Janeiro, RJ (Brazil)

    2017-07-01

    {sup 60}Co is an artificial radioactive metal produced by activation of iron with neutrons. It decays by beta particles and gamma radiation and represents a risk of internal exposure of workers involved in the maintenance of nuclear power reactors. Intakes can be quantified through in vivo monitoring. This work describes the development of a technique for the quantification of {sup 60}Co in human liver. The sensitivity of the method is evaluated based on the minimum detectable effective doses. The results allow to state that the technique is suitable either for monitoring of occupational exposures or evaluation of accidental intakes. (author)

  20. Dosimetric evaluation of Radiotherapy units wit {sup 60}Co; Evaluacion dosimetrica de unidades de radioterapia con {sup 60}Co

    Energy Technology Data Exchange (ETDEWEB)

    Leon, B. Salinas de; Tovar M, V.; Becerril V, A. [Instituto Nacional de Investigaciones Nucleares, A.P. 18-1027, 11801 Mexico D.F. (Mexico)

    2000-07-01

    The SSDL network of the IAEA performs, every year, quality audit tests for radiotherapy services ({sup 60} Co units and linear accelerators), and for national SSDL as well. Because of the SSDL-Mexico results in these tests and due to our enthusiasm and confidence in our work, a parallel test has been done , which is described in this talk as well as the results. Nowadays, a second parallel test goes up, which could confirm our optimism and open the possibility to our country to start a national dosimetric audit of {sup 60} Co radiotherapy units. (Author)

  1. Decoloration Kinetics of Waste Cooking Oil by 60Co γ-ray/H2O2

    Science.gov (United States)

    Xiang, Yulin; Xiang, Yuxiu; Wang, Lipeng

    2016-03-01

    In order to decolorize, waste cooking oil, a dark red close to black solution from homes and restaurants, was subjected to 60Co γ-ray/H2O2 treatment. By virtue of UV/Vis spectrophotometric method, the influence of Gamma irradiation to decoloration kinetics and rate constants of the waste cooking oil in the presence of H2O2 was researched. In addition, the influence of different factors such as H2O2 concentration and irradiation dose on the decoloration rate of waste cooking oil was investigated. Results indicated that the decoloration kinetics of waste cooking oil conformed to the first-order reaction. The decoloration rate increased with the increase of irradiation dose and H2O2 concentration. Saponification analysis and sensory evaluation showed that the sample by 60Co γ-ray/H2O2 treatment presented better saponification performance and sensory score. Furthermore, according to cost estimate, the cost of the 60Co γ-ray/H2O2 was lower and more feasible than the H2O2 alone for decoloration of waste cooking oil.

  2. Phycoremediation of 137Cs and 60Co with selected species of aquatic microalgae

    International Nuclear Information System (INIS)

    Tatarova, D.; Galanda, D.; Strisovska, J.

    2016-01-01

    The presentation is focused on finding a suitable pH for phycoremediation of 137 Cs and 60 Co w ith microalgae Dunaliella salina and Chlorella vulgaris. To ensure a dynamic course of remediation the peristaltic pump was used, through which the solution was washed with radionuclides. During individual measurements the decrease in solution activity over time was monitored . Decline in activity in the samples was determined using a semiconductor HPGe gamma detector. The measured results showed that the best environment for phycoremediation for microalgae Dunaliella salina was at pH = 8 and less, for Chlorella vulgaris the best value was pH = 6. (authors)

  3. Study of recombination processes for 'electron-hole' pairs in germanium irradiated by {gamma} rays from {sup 60}Co using the photovoltaic effect in P-N junctions; Etude du processus de recombinaison des paires ''electron-trou'' dans le germanium irradie par les rayons {gamma} du cobalt 60 a l'aide de l'effet photovoltaique dans les jonctions P-N

    Energy Technology Data Exchange (ETDEWEB)

    Zahedi-Mochadam, A A [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1964-10-01

    Using the photo-voltaic effect in p-n junctions, we have studied, during bombardment, the mechanism of the recombination of 'electron-hole' pairs in the presence of structure defects produced in germanium of the N and P types by {gamma} rays from a Co{sup 60} source. At 310 K the level of the recombination centres is situated 0.25 eV above the conduction band and the capture cross-sections of the holes and of the electrons have the respective values of: {sigma}{sub p} = 4 X 10{sup -15} cm{sup 2} and {sigma}{sub n} = 3 X 10{sup -15} cm{sup 2}. The value of {sigma}{sub n} appears to be under-estimated because the number of defects in P-type samples appears to be lower than that in N-type samples. These results lead to the conclusion that the interstitials are responsible for the recombination. At 80 K it has been found that in N-type samples, a shallow level exists at O.05 eV below the conduction band with a capture cross-section for the holes of {sigma}{sub p} {>=} 10{sup -14} cm{sup 2}. We believe that in this case the recombination of charge carriers is controlled by the neighbouring 'defect-interstitial' pairs. In P-type samples at low temperature, the life-time is practically constant during irradiation. This fact is attributed to a spontaneous annealing of defects ol purely electrical origin. In the last part of the work the study of the photo-voltaic effect applied to the problem of gamma radiation dosimetry is considered. It is shown that such dosimeters, based on this principle, make it possible to measure the intensity of gamma rays over a very wide range. (author) [French] En utilisant l'effet photovoltaique dans les jonctions p-n, nous avons etudie au cours du bombardement le mecanisme de recombinaison des paires 'electron-trou' en presence des defauts de structure introduits dans le germanium de type N et de type P par les rayons gamma d'une source de Co{sup 60}. A 310 K, le niveau des centres de recombinaison se trouve a 0,25 eV au-dessous de la bande

  4. The accumulation and distribution of 60Co in carp (Cyprinus carpio) in water-fish compartment system

    International Nuclear Information System (INIS)

    Poppy Intan Tjahaja; Putu Sukmabuana; Eko Susanto

    2012-01-01

    In relation with nuclear safety assessment, the parameter of radionuclide transfer in the environment is significantly needed for internal doses estimation received by public trough environment - food product - human pathways. International Atomic Energy Agency (IAEA) has published the transfer parameter data for temperate zone in Technical Report Series 472. In order to complete the IAEA data, especially for tropical region, the accumulation and distribution of 60 Co in carp (Cyprinus carpio) was experimentally studied based on the water-fish compartment system. The carp were cultured in 500L water containing 60 Co of about 30 Bq.mL -1 in a water tank. The 60 Co concentration in fish and water were measured using gamma spectrometer. The 60 Co was accumulated and distributed in the fish tissues with the concentration ratio (CR) of 3.08 mL.g -1 1.55 mL.g -1 and 1.14 mL.g -1 for internal organs, bones, and muscle, respectively. The CR of 60 Co in the fish will be useful in internal radiation dose estimation to human trough water-fish-human pathway, and will also complete the IAEA transfer parameter data for tropical region. (author)

  5. Seed germination of peanuts irradiated with cobalt ({sup 60}CO); Germinacao de sementes de amendoim irradiadas com cobalto ({sup 60}Co)

    Energy Technology Data Exchange (ETDEWEB)

    Alves, Niedja Marrize C.; Almeida, Francisco de Assis C.; Gomes, Josivanda P.; Pessoa, Elvira B., E-mail: niedjamarizze@yahoo.com.b, E-mail: josivanda@deag.ufcg.edu.b, E-mail: elvirabe@gmail.co [Universidade Federal de Campina Grande (UFCG), PB (Brazil). Dept. de Engenharia Agricola; Leal, Artur S. Cavalcanti, E-mail: arturcleal@yahoo.com.b [Universidade Federal de Campina Grande (UFCG), PB (Brazil). Dept. de Engenharia de Materiais

    2010-07-01

    This work was realized to evaluate the effect of gamma irradiation ({sup 60}Co) at doses 0, 0.50, 1.00, 1.50, 2.00, 2.50, 3.00 and 4.00 kGy, on germination of seeds of peanut, cultivar BR1. Irradiation Department of Nuclear, UFPE, where he received after the irradiation, they were stored in packing of PET and polyethylene braided with a time of 90 days. Through the results, obtained monthly, concluded that the dose of 0.5 kGy was effective in the germination of seeds of peanut, not affecting its power of germination and overcoming the witness within 30 to 60 days. (author)

  6. Biological effect of 60Co γ irradiation on Trichoderma

    International Nuclear Information System (INIS)

    Chen Jian'ai; Wang Weiming

    2003-01-01

    The mutagenic effect of 60 Co γ-ray on Trichoderma spp (T 32 ) was studied. The germination capacity of spores apparently declined after irradiation. The mutagenic effect curve model is S=e -λD , and the mutagenic curve is Y=-0.940e 1.29x , r=0.9533(r 0.01 =0.765). 130 variant strain which grew rapidly and produced more spores were chosen

  7. Image processing in 60Co container inspection system

    International Nuclear Information System (INIS)

    Wu Zhifang; Zhou Liye; Wang Liqiang; Liu Ximing

    1999-01-01

    The authors analyzes the features of 60 Co container inspection image, the design of several special processing methods for container image and some normal processing methods for two-dimensional digital image, including gray enhancement, pseudo-enhancement, space filter, edge enhancement, geometry process, etc. It gives out the way to carry out the above mentioned process in Windows 95 or Win NT. It discusses some ways to improve the image processing speed on microcomputer and good results were obtained

  8. Effects of 60Co administration on early placental cells

    International Nuclear Information System (INIS)

    Honda, Jin

    1979-01-01

    The effects of 60 Co administration on early placental cells were studied. Placental tissue and embryo obtained by induced abortion (6 - 13 weeks gestational age) were placed in the minimal essential medium (MEM) and irradiated with various doses of 60 Co. After irradiation, the villi were cultured in a CO 2 incubater at 37 0 C. Cell growth process was observed every day with the phase-contrast microscope. Between 1 and 5 days epitheloid cells were dominant, but from about 7th day on fibroblastic cells dominated the culture. In placental tissue irradiated with 100, 200, 500 rad, fibroblastic cells began to grow earlier than in non-treated. Over 3000 rad 60 Co inhibited the growth of cells and a culture was impossible. For each dose, the tissue was incubated for various periods of time, exposed to tritiated thymidine for the last hour and autoradiogram was prepared by the dipping method. The labeling index of irradiated trophoblasts showed a significant decrease compared with controls. A chromosome study was made in irradiated in vitro cell lines of fetus and placenta. There was no significant difference between the two cell lines concerning the frequency of chromosome aberration, which tended to increase as the chromosome becomes longer. It is concluded that the trophoblast is highly radiosensitive and that irradiation early in pregnancy may damage DNA synthesis in the trophoblast, and induce abortion. (author)

  9. Sensitivity of watermelon variety Bojura to mutant agents 60Co and EMS

    Directory of Open Access Journals (Sweden)

    Nikolay Velkov

    2016-04-01

    Full Text Available A study on the sensitivity of watermelon variety Bojura to mutagenic agents was carried out in 2013-2014. The goal was to establish effective doses for mutagenic treatment of dry seeds with 60Co gamma rays (80, 100, 200, 250, 350 and 450 Gy and swollen seeds with water for 24 hours were treated with ethyl methanesulfonate (EMS at a concentration of 2%. Dominant mutations were not observed in the M1 generation. Morphological changes in 14 of 1395 M2 plants were observed. Phenotypic variations changes were the colour of the seed coat, chlorophyll disorders of cotyledons, leaves, petals, and alterations of the location of the fruit set in the central stem. Visible changes of the morphological characteristics of the fruit were not observed. The doses induced certain morphological changes, however, higher doses or combined gamma rays 60Co and EMS treatments would induce mutations more efficiently. Subsequent experiments are required to obtain mutants with changes that affect flowers and fruits. The results are important for increasing mutation efficiency in watermelon breeding.

  10. Evaluation of radiation protection after the alteration of 60Co irradiation equipment and the augmentation of 60Co load volume

    International Nuclear Information System (INIS)

    Shi Hongfu

    1990-01-01

    In order to carry out the irradiation sterilization of medical devices, the authors have augmented the 60 Co load volume to 2.32 pBq, improved the properties of the radiation field and altered the radiation room. All these changes have been checked and accepted by the authorities concerned of Jiangsu Province and Suzhou City. The alteration increases safety and improves the uniformity of high-range dose distribution in the radiation field. The uniformity turns from the original 1.95 to the present 1.34 within the height of 2000 mm from the ground surface and thus can be compared with that of automatic irradiation conveyor abroad. The alteration makes it possible to carry out irradiation treatment of industrial scale by means of 60 Co irradiation equipment designed for biomedial experiments

  11. Set of devices for producing radioactive 60Co-sources

    International Nuclear Information System (INIS)

    Eichhorn, P.; Tobisch, F.

    1982-01-01

    A set of devices for producing radioactive 60 Co-sources was developed. A single source has a radioactivity of 445x10 10 GBq. It consists of a double envelope of stainless steel filled with a mixture of small pieces of cobalt and stainless steel wire. The diameter of a source is 11 mm; the length 80 mm. Cobalt wires of different radioactivity with a length of about 110 mm and 0,8 mm diameter are the raw material. The set is located in a hot cell. Construction, functions and operation of the set are described in detail. (author)

  12. Health Condition of Individuals in Contact with 60 Co

    International Nuclear Information System (INIS)

    Milacic, S.; Jovicic, D.; Bulat, P.

    1997-01-01

    Individuals who are not professionally exposed to ionizing radiation may have the risk of contact with radioactive substances by nature of teir profession. Our aim is ti suggest a methodology for evaluation of immediate health impairment and possible late consquences of exposure of these individuals. Except for anamnestic, clinical and general laboratory indicators we analyzed cell morphology, enzymes and chromosomal aberration as well as indicators of free radicals concentrations in 62 which were in contact with 60 Co. The obtained results and evaluation of exposure and absorbed doses indicate possible consequaences in 8 subjects who are close to the source. (author)

  13. Sorption of 60 Co in natural zeolite (clinoptilolite)

    International Nuclear Information System (INIS)

    Hernandez B, E.

    1996-01-01

    A Mexican zeolite (clinoptilolite) from Taxco, Guerrero, was partially stabilized with sodium cations. Radioactive Cobalt ( 60 Co) was used to study the Co 2+ sorption in the stabilized zeolite (Na + ). It was found that sorption in general does not favour the diffusion of cobalt between framework, it explains because of it is a natural zeolite and its composition heterogeneous decrease its exchange capacity by the generated competence to the existence other type of exchange ions. The cobalt retention reached the highest level, around 0.408 m eq Co 2+ /g in the Na-Clinoptilolite. The crystallinity of the aluminosilicates was maintained during experiments, it was verified by XRD patterns. (Author)

  14. Measurements of {sup 60}Co in spoons activated by neutrons during the JCO criticality accident at Tokai-mura in 1999

    Energy Technology Data Exchange (ETDEWEB)

    Gasparro, J.; Hult, M. E-mail: mikael.hult@irmm.jrc.be; Komura, K.; Arnold, D.; Holmes, L.; Johnston, P.N.; Laubenstein, M.; Neumaier, S.; Reyss, J.-L.; Schillebeeckx, P.; Tagziria, H.; Van Britsom, G.; Vasselli, R

    2004-07-01

    Neutron activated items from the vicinity of the place where the JCO criticality accident occurred have been used to determine the fluence of neutrons around the facility and in nearby residential areas. By using underground laboratories for measuring the activation products, it is possible to extend the study to also cover radionuclides with very low activities from long-lived radionuclides. The present study describes {gamma}-ray spectrometry measurements undertaken in a range of underground laboratories for the purpose of measuring {sup 60}Co more than 2 years after the criticality event. The measurements show that neutron fluence determined from {sup 60}Co activity is in agreement with previous measurements using the short-lived radionuclides {sup 51}Cr and {sup 59}Fe. Limits on contamination of the samples with {sup 60}Co are evaluated and shown to not greatly affect the utility of neutron fluence determinations using {sup 60}Co activation.

  15. Humoral immune response against native or 60Co irradiated venom and mucus from stingray Paratrygon aiereba

    International Nuclear Information System (INIS)

    Thomazi, Gabriela Ortega Coelho; Alves, Glaucie Jussilane; Aires, Raquel da Silva; Turibio, Thompson de Oliveira; Rocha, Andre Moreira; Spencer, Patrick Jack; Nascimento, Nanci do; Seibert, Carla Simone

    2015-01-01

    Poisonings and traumas caused by poisonous freshwater fish such as rays are considered a major public health problem and draw attention because of accidents involving these animals cause serious local symptoms and are disabling, keeping the victim away from work. The therapy of these cases is based only on the symptoms of patients, which implies in its low efficiency, causing suffering for the victims. This study aims to evaluate and compare the humoral immune response in animals inoculated with native or 60 Co irradiated Paratrygon aiereba venom and mucus. Ionizing radiation has proven to be an excellent tool to decrease the toxicity of venoms and isolated toxins. The mucus and venom samples of P. aiereba were irradiated using gamma rays from a 60 Co source. Animals models were immunized with the native or irradiated mucus or venom. The assays were conducted to assess the production of antibodies by the immunized animals using enzyme immunoassay and western blotting. Preliminary results show the production of antibodies by the immunized animals. The resulting sera were also checked for antigenic cross- reactivity between venom and mucus, demonstrating the potential of mucus as an antigen for serum production for the specific treatment for accidents by stingrays. However, it is essential to carry out further tests in order to verify the neutralization of the toxin by antibodies formed by animals. (author)

  16. Biochemical and hematological study of goats envenomed with natural and 60Co-irradiated bothropic venom

    International Nuclear Information System (INIS)

    Lucas de Oliveira, P.C.; Madruga, R.A.; Barbosa, N.P.U.; Sakate, M.

    2007-01-01

    Venoms from snakes of the Bothrops genus are proteolytic, coagulant, hemorrhagic and nephrotoxic, causing edema, necrosis, hemorrhage and intense pain at the bite site, besides systemic alterations. Many adjuvants have been added to the venom used in the sensitization of antiserum-producer animals to increase antigenic induction and reduce the envenomation pathological effects. Gamma radiation from 60 Co has been used as an attenuating agent of the venoms toxic properties. The main objective was to study, comparatively, clinical and laboratory aspects of goats inoculated with bothropic (Bothrops jararaca) venom, natural and irradiated from a 60 Co source. Twelve goats were divided into two groups of six animals: GINV, inoculated with 0.5 mg/kg of natural venom; and GIIV, inoculated with 0.5 mg/kg of irradiated venom. Blood samples were collected immediately before and one, two, seven, and thirty days after venom injection. Local lesions were daily evaluated. The following exams were carried out: blood tests; biochemical tests of urea, creatinine, creatine kinase, aspartate amino-transferase and alanine amino-transferase; clotting time; platelets count; and total serum immunoglobulin measurement. In the conditions of the present experiment, irradiated venom was less aggressive and more immunogenic than natural venom. (author)

  17. Biochemical and hematological study of goats envenomed with natural and 60Co-irradiated bothropic venom

    Energy Technology Data Exchange (ETDEWEB)

    Lucas de Oliveira, P.C.; Madruga, R.A.; Barbosa, N.P.U. [Uberaba School of Veterinary Medicine (UNIUBE), MG (Brazil)]. E-mail: pedrolucaso@uol.com.br; Sakate, M. [UNESP, Botucatu, SP (Brazil). School of Veterinary Medicine and Animal Husbandry

    2007-07-01

    Venoms from snakes of the Bothrops genus are proteolytic, coagulant, hemorrhagic and nephrotoxic, causing edema, necrosis, hemorrhage and intense pain at the bite site, besides systemic alterations. Many adjuvants have been added to the venom used in the sensitization of antiserum-producer animals to increase antigenic induction and reduce the envenomation pathological effects. Gamma radiation from {sup 60}Co has been used as an attenuating agent of the venoms toxic properties. The main objective was to study, comparatively, clinical and laboratory aspects of goats inoculated with bothropic (Bothrops jararaca) venom, natural and irradiated from a {sup 60}Co source. Twelve goats were divided into two groups of six animals: GINV, inoculated with 0.5 mg/kg of natural venom; and GIIV, inoculated with 0.5 mg/kg of irradiated venom. Blood samples were collected immediately before and one, two, seven, and thirty days after venom injection. Local lesions were daily evaluated. The following exams were carried out: blood tests; biochemical tests of urea, creatinine, creatine kinase, aspartate amino-transferase and alanine amino-transferase; clotting time; platelets count; and total serum immunoglobulin measurement. In the conditions of the present experiment, irradiated venom was less aggressive and more immunogenic than natural venom. (author)

  18. Lesson from a 60Co source radiation accident

    International Nuclear Information System (INIS)

    Guo Yong; Zhang Wenzhong

    2002-01-01

    A serious radiation accident happened an a 60 Co irradiation facility in Shanghai. 7 workers were uniformly exposed acutely. An investigation was done after the accident and a conclusion was achieved that the irregular operation was the direct reason for the accident. The operation of these workers did not comply with the requirements specified in the national standards-- 60 irradiation facility>> which demands that the examination should be done every day before operation, and the irradiation facility does not stop running when the auto-lock safety system on that facility has been removed. Some lessons should be drawn from the accident: popularizing the culture of safety, enhancing the law of safety, and ensuring the operation of radiation devices within the demands of safety

  19. Planning a 60Co irradiation facility for fruit preservation

    International Nuclear Information System (INIS)

    Yuan, H.C.

    1978-01-01

    A conceptual design for a conveyor system is proposed for use in fruit irradiation. The seasonal nature of the fruit harvest requires that the 60 Co source inventory should be sufficient to meet the demand at peak season, but this would be excessive at the beginning and towards the end of the harvest. Because of the short crop period the possibility of other irradiation services should be exploited to ensure full utilization of the facility. For successful extension of fruit shelf-life rigid practices in pre-irradiation treatment are essential and careful packaging is indispensable to the operation of the irradiator. Based on the time required for construction and equipment supply, a period of 18 months should be assumed for completion of the project. (author)

  20. Fieldbus: technology application in a 60Co sterilization plant

    International Nuclear Information System (INIS)

    Karam, D.; Sampa, M.H.O.; Rela, P.R.

    2000-01-01

    Process instrumentation was made by pressure signals in the 1940s. In the 1960s, 4-20 mA analogue signals were introduced. The development of digital processors in the 1970s sparked the use of computers to monitor and control instruments from a central point. In the 1980s smart sensors were developed and implemented in digital control, microprocessor environments. Fieldbus is a generic-term that describes a new digital communications network. The network is a digital, bi-directional, multidrop, serial-bus, and communications network used to link isolated field devices, such as controllers, transducers, actuators and sensors. Fieldbus technology may improve quality, reduce costs and increase efficiency because information is transmitted digitally without analog to digital or digital to analog converters, which also minimizes hardware errors. Fieldbus communication is based on two-wire communication, interconnecting all the components in the system. This paper introduces Fieldbus technology in a 60 Co sterilization plant

  1. Delayed match-to-sample early performance decrement in monkeys after 60Co irradiation

    International Nuclear Information System (INIS)

    Bruner, A.; Bogo, V.; Jones, R.K.

    1975-01-01

    Sixteen monkeys were trained on a delayed match-to-sample task (DMTS) based on shock avoidance and irradiated with single, whole-body exposures of from 396 to 2000 rad 60 Co (midbody dose) at between 163 and 233 rad/min. Pre- to post-irradiation performance changes were assessed using a penalty-scaling measure which differentially weighted incorrect responses, response omissions, and error-omission sequences. Thirteen of the animals displayed early performance decrement, including five incapacitations, at lower doses (less than 1000 rad) than heretofore found effective. This was considered a function of task complexity, measurement sensitivity, and gamma effectiveness. The minimum effective midbody dose for inducing decrement using the DMTS task was estimated to be on the order of 500 rad. The nature of early, transient performance decrement seems to reflect more of an inability to perform than an inability to perform correctly

  2. Analysis of the frequency of unstable chromosome aberrations in human lymphocytes irradiated with 60Co

    International Nuclear Information System (INIS)

    Mendonca, Julyanne C.G.; Mendes, Mariana E.; Lima, Fabiana F.; Santos, Neide

    2013-01-01

    The aim of this study was to analyze the frequency of unstable chromosomal aberrations induced by gamma radiation from a 60 Co source at two different doses. Samples were obtained from a healthy donor and exposed to 60 Co source (Gammacel 220 ) located in the Department of Nuclear Energy of Pernambuco Federal University (DEN/UFPe/Brazil) with a rate of air Kerma to 3,277 Gy/h. Exposures resulted in absorbed dose 0.51 Gy and 0.77 Gy. Mitotic metaphases were obtained by culturing lymphocytes for chromosome analysis and the slides were stained with 5% Giemsa. Among the unstable chromosomal aberrations the dicentric chromosomes, ring chromosomes and acentric fragments were analyzed. To calculate the significance level the chi - square test was used, considering relevant differences between the frequencies when the value of p < 0.05. To calculate the significance level of the chi - square test was used, considering relevant differences between the frequencies when the value of p < 0.05. The results showed that there was significant difference of the frequencies of dicentric chromosomes (from 0.18 to 0.51 to 0.37 Gy to 0.77 Gy), however there was no statistically significant difference between the frequencies of acentric fragments ( 0.054 to 0, 51 Gy to 0.063 to 0.77 Gy) and ring chromosomes (0.001 to 0.51 Gy to 0.003 to 0.77 Gy). The low number of rings is found justified, considering that in irradiated human lymphocytes, its appearance is rare relative to dicentrics. The results confirm that dicentrics are the most reliable biomarkers in estimating dose after exposure to gamma radiation. These two points will make the calibration curve dose-response being built for Biological Dosimetry Laboratory of CRCN-NE/CNEN

  3. 129I, 60Co, and 106Ru measurements on water samples from the Hanford project environs

    International Nuclear Information System (INIS)

    Brauer, F.P.; Rieck, H.G. Jr.

    1973-01-01

    Groundwater flow and contamination patterns beneath the Hanford project reservation have been studied since the early days of the project. The measurement of radioactive materials at concentrations much below those required for radiation protection are useful for tracing groundwater movement and detection of potential contamination problems before they are apt to occur. Groundwater samples from a number of wells on or near the Hanford reservation have been analyzed for 129 I by neutron activation analysis and for gamma radioactivity by low-level coincidence gamma-ray spectrometry. The major radionuclides in addition to natural radioactivity detected in the underground waters by gamma-ray spectrometry were 106 Ru and 60 Co. Local river and rain water samples were also analyzed for 129 I and long-lived radionuclides. Special sample collection methods were developed to prevent contamination of the water samples during collection. Anions travel farther than cations in underground water systems since soils are primarily cation exchangers and retain the cations. Anion exchange techniques were used in the field and the laboratory to recover the desired radionuclides. Sample sizes ranged up to several thousand liters. This paper discusses the sample collection methods,analysis methods, and results obtained. The methods used were found to provide high sensitivity for groundwater studies. (auth)

  4. Monte Carlo investigation of design aspects of indigenously developed 120 Ci 60Co based industrial radiography device

    International Nuclear Information System (INIS)

    Palam Selvam, T.; Vishwakarma, R.S.; Sahoo, Dhiren K.; Sharma, Manoj K.; Srivastava, Piyush

    2016-01-01

    Industrial Radiography is an indispensable, versatile and well-established non destructive testing (NDT). Industrial radiography is carried out by using industrial radiography gamma exposure devices (IGRED) housing 60 Co and 192 Ir radioactive sources, and Industrial X-ray machines/accelerators. IGRED is an assembly of components which includes source housing, exposure mechanism, source drive system, pig tail, and source conduit. In the present study, we investigated the shielding design aspects of 120 Ci 60 Co-based IRGED using the Monte Carlo methods (MCNP version 3.1). The design details were provided by Board of Radiation and Isotope Technology (BRIT). As the objective is to finalize the suitable design of the device, we also included additional designs. The work also included measurements around the device using TLDs

  5. Detection and thermoluminescence of coriander (Coriandrum sativum L.) irradiated with {sup 60}Co; Deteccion y termoluminiscencia del cilantro (Coriandrum sativum L.) irradiado con {sup 60}Co

    Energy Technology Data Exchange (ETDEWEB)

    Ruiz G, B. [Departamento de Agricultura y Ganaderia, Universidad de Sonora, 083190 Hermosillo, Sonora (Mexico); Cruz Z, E. [Unidad de Irradiacion y Seguridad Radiologica, Instituto de Ciencias Nucleares, UNAM, A. P. 70-543, 04510 Mexico D. F. (Mexico); Barboza F, M. [Centro de Investigacion en Fisica, Universidad de Sonora, A. P. 5-088, 083190 Hermosillo, Sonora (Mexico)

    2009-10-15

    The fresh and dried coriander (Coriandrum sativum L.) is a spice of high commercial value as food for consumption in Mexico. Their hygienic quality is often poor due to some factors: contaminants present in irrigation water and the cultivation atmosphere, harvesting and post harvest handling, mainly. The process by radiation is an alternative to achieve the sterilization to adequate dose for the hygienic quality for its consumption. However, irradiation also involves food detection exposed to radiation. This paper presents detected results on the dried coriander that was obtained from fresh samples and thermoluminescent properties such as glow curves structure from low doses (0.5 Gy) to high (15 kGy), the dose response, thermoluminescent signal decay, in order to determine the loss of stability during the storage of the poly mineral fraction. We obtained the inorganic fraction separation of the organic part of particle sizes of 10 {mu}m by the Zimmerman method. The samples were exposed at two dose ranges; 0.5-400 Gy and 0.5-15 kGy of gamma radiation with {sup 60}Co. The limit of thermoluminescent detection of the irradiated coriander samples was from 1 Gy. The glow curves were at a broad band of 35-400 C, with a maximum thermoluminescence around 182-196 C, and run at 164 C for high doses of 15 kGy. The range of linear response to dose was 4-25 Gy, whereas higher doses than the kGy order the response increase with the dose. The thermoluminescent properties of the coriander poly mineral fraction, show it can be used to identify irradiated food at gamma doses relatively low and even in doses of commercial interest due to the high stability of thermoluminescent signals. (Author)

  6. Effect of {sup 60}CO radiation processing in mate (Ilex paraguariensis); Efeito do processamento por radiacao de {sup 60}CO na erva-mate (Ilex paraguariensis)

    Energy Technology Data Exchange (ETDEWEB)

    Furgeri, Camilo

    2009-07-01

    The mate (Ilex paraguariensis), a native species from South America, is mainly consumed as typical beverage called chimarrao and terere. An important problem that has been afflicting this product since a long time is its natural fungal contamination responsible to affect its physical, health and nutritional qualities. In order to improve this product quality, radiation processing can be effective in reducing pathogens levels, with minimal nutritional and sensory changes. The aim of this study was to evaluate the effectiveness of gamma radiation from {sup 60}Co at doses 0, 3, 5, 7 and 10kGy in reducing fungal contamination in mate, as well as analyze its nutritional and sensory characteristics. The following methodologies were applied: analysis of yeast and mold, total phenolic compounds analysis, antioxidant analysis, quantification of phenolic compounds and xanthines by liquid chromatography and sensory analysis. Microbiological analysis showed a decreasing molds and yeasts growth with increasing radiation doses. Regardless of the radiation dose applied there were no decrease of total phenolic compounds in both infusions. Chimarrao samples irradiated with 7 and 10kGy showed a decrease in the DPPH radical-scavenger activity, nevertheless for terere samples, there were no significant difference. Chimarrao chromatographic profile did not show a variation on xanthines quantification, however a 10kGy radiation dose caused a change to phenolic compounds quantitative profile. Terere samples did not show any significant difference to any analyzed compounds. Sensory analysis did not exhibit a significant difference between irradiated and non irradiated chimarrao samples, as well as between irradiated and non irradiated terere samples. It could be concluded that gamma radiation processing of mate may be a feasible alternative to industry, since there was a reduction on fungal contamination, without changes in sensory qualities and with minimum alterations in quantitative

  7. Effect of {sup 60}Co radiation processing in mate (Ilex paraguariensis); Efeito do processamento por radiacao de {sup 60}Co na erva-mate (llex paraguariensis)

    Energy Technology Data Exchange (ETDEWEB)

    Furgeri, Camilo

    2009-07-01

    The mate (Ilex paraguariensis), a native species from South America, is mainly consumed as typical beverage called chimarrao and terere. An important problem that has been afflicting this product since a long time is its natural fungal contamination responsible to affect its physical, health and nutritional qualities. In order to improve this product quality, radiation processing can be effective in reducing pathogens levels, with minimal nutritional and sensory changes. The aim of this study was to evaluate the effectiveness of gamma radiation from {sup 60}Co at doses 0, 3, 5, 7 and 10 kGy in reducing fungal contamination in mate, as well as analyze its nutritional and sensory characteristics. The following methodologies were applied: analysis of yeast and mold, total phenolic compounds analysis, antioxidant analysis, quantification of phenolic compounds and xanthines by liquid chromatography and sensory analysis. Microbiological analysis showed a decreasing molds and yeasts growth with increasing radiation doses. Regardless of the radiation dose applied there were no decrease of total phenolic compounds in both infusions. Chimarrao samples irradiated with 7 and 10 kGy showed a decrease in the DPPH radical-scavenger activity, nevertheless for terere samples, there were no significant difference. Chimarrao chromatographic profile did not show a variation on xanthines quantification, however a 10 kGy radiation dose caused a change to phenolic compounds quantitative profile. Terere samples did not show any significant difference to any analyzed compounds. Sensory analysis did not exhibit a significant difference between irradiated and non irradiated chimarrao samples, as well as between irradiated and non irradiated terere samples. It could be concluded that gamma radiation processing of mate may be a feasible alternative to industry, since there was a reduction on fungal contamination, without changes in sensory qualities and with minimum alterations in quantitative

  8. Activation measurements for thermal neutrons. Part A. Cobalt (60Co) activation

    International Nuclear Information System (INIS)

    Kerr, George D.; Shizuma, Kiyoshi; Endo, Satoru; Maruyama, Takashi; Cullings, Harry M.; Komura, Kazuhisa; Okumura, Yutaka; Egbert, Stephen D.

    2005-01-01

    The 60 Co measurements at Hiroshima and Nagasaki were reviewed and compared with the results of the new DS02 calculations for the two cities. Some corrections were made to previously published data from the 1965 measurements of JNIRS, ground ranges of all of the 60 Co measurements were reviewed and new ground ranges were determined when possible by transforming sample coordinates to the new city maps for Hiroshima and Nagasaki, and transmission factors were investigated for a number of samples used in the 60 Co measurements. Transmission factors were not calculated for all samples used in the 60 Co measurements but some general rules were given for estimating transmission factors for these samples. Experimental measurements of the background 60 Co activity from environmental neutrons were also reviewed, and it was found that the 60 Co background from environmental neutrons was not an important factor in the 60 Co measurements of bomb-induced activity at either city. (J.P.N.)

  9. Dose response curve for prematurely condensed chromosome fragments of human lymphocytes after 60Co-γ ray exposure

    International Nuclear Information System (INIS)

    Gao Jinsheng; Zheng Siying; Bao Hong

    1992-06-01

    The dose-effect relationship of premature condensed chromosome fragments in human lymphocytes irradiated by 60 Co gamma ray was studied by PCC method (premature condensed chromosomes). In addition, The conventional cellular genetics method was also used in the study. The response curve of both methods can represented by two linear equations. The ratio of two slopes, K PCC /K M1 , is about 28. Comparing with conventional method, the PCC method has many advantages such as faster, simpler, more sensitive and accurate. The PCC method used in the studying of radiation damage is also discussed

  10. Comparison of the standards for absorbed dose to water of the ARPANSA and the BIPM for 60Co γ radiation

    International Nuclear Information System (INIS)

    Allisy-Roberts, P.J.; Burns, D.T.; Boas, J.F.; Huntley, R.B.; Wise, K.N.

    2000-10-01

    A comparison of the standards for absorbed dose to water of the Australian Radiation Protection and Nuclear Safety Agency and of the Bureau International des Poids et Mesures (BIPM) has been carried out in 60 Co gamma radiation. The Australian standard is based on a graphite calorimeter and the subsequent conversion from absorbed dose to graphite to absorbed dose to water using the photon fluence scaling theorem. The BIPM standard is ionometric using a graphite-walled cavity ionization chamber. The comparison result is 1.0024 (standard uncertainty 0.0029). (authors)

  11. Variation of photon intensities in transmitted photon spectra of 60Co as a function of dimensions of a soil medium

    International Nuclear Information System (INIS)

    Singh, Charanjeet; Sidhu, Gurdeep S.; Kumar, Ashok; Singh, Tejbir; Singh, Parjit S.; Mudahar, Gurmel S.

    2005-01-01

    The transmitted photon spectra of a point gamma-ray source of 60 Co have been measured through the longitudinal and transverse dimensions of a soil medium. The simultaneous effect of longitudinal and transverse thicknesses has been studied on the intensity of an appeared multiple-scatter peak in the soft part of the spectrum. The intensity of peak decreases exponentially with the increase of longitudinal thickness of soil medium whereas a reversal of the trend of multiple-scatter peak intensity with the transverse thickness observed as longitudinal dimension increases

  12. Viabilidade e micoflora de sementes de amendoim irradiadas com cobalto (60Co Viability and mycoflora of irradiated seeds of peanut with cobalt (60Co

    Directory of Open Access Journals (Sweden)

    Niédja M. C. Alves

    2011-03-01

    Full Text Available Estudou-se o efeito de diferentes doses da irradiação gama na germinação e percentagem de infecção fúngica em sementes de amendoim cultivar BR1. Inicialmente, as sementes foram avaliadas quanto à micoflora, utilizando-se o método de papel de filtro umedecido, a germinação em substrato de papel germitest e determinação do teor de umidade em estufa, a 105 ± 2 ºC. Posteriormente, as sementes foram submetidas à irradiação com uma fonte de 60Co, tipo gammacell, onde se estudou o efeito de 8 doses de irradiação na micoflora e germinação das sementes acondicionadas em embalagem de PET e polietileno trançado durante 60 dias de armazenamento, sem controle de temperatura e umidade relativa do ar. Os fatores estudados (doses em kGy, embalagens e tempo de armazenamento foram analisados em um delineamento inteiramente casualizado, dispostos em fatorial. Com base nos resultados, observou-se que a irradiação gama afetou negativamente a germinação das sementes de amendoim e que doses acima de 3,0 kGy comprometem a germinação das sementes. A radiação a partir da dose 1,5 kGy eliminou os fungos Aspergillus niger e, Penicillium a partir da dose de 2,5 kGy.The effect of different doses of the gamma irradiation was studied on germination and percentage of fungal infection in peanut seeds cultivar BR1. Initially the seeds were evaluated for the mycoflora using the method of humidified filter paper, the germination in paper substratum germitest and determination of the moisture content in oven at 105 ± 2 ºC. Later on the seeds were submitted to irradiation with a source of 60Co, type gammacell, where the effect was studied of 8 doses of irradiation in the mycoflora and germination of the seeds conditioned in PET packing and polyethylene tressed during 60 days of storage, without control of temperature and relative humidity of the air. The studied factors (doses in kGy, packing and time of storage were analyzed in a completely

  13. Bioremediation of {sup 60}Co from simulated spent decontamination solutions

    Energy Technology Data Exchange (ETDEWEB)

    Rashmi, K.; Naga Sowjanya, T.; Maruthi Mohan, P.; Balaji, V.; Venkateswaran, G

    2004-07-26

    Bioremediation of {sup 60}Co from simulated spent decontamination solutions by utilizing different biomass of (Neurospora crassa, Trichoderma viridae, Mucor recemosus, Rhizopus chinensis, Penicillium citrinum, Aspergillus niger and, Aspergillus flavus) fungi is reported. Various fungal species were screened to evaluate their potential for removing cobalt from very low concentrations (0.03-0.16 {mu}M) in presence of a high background of iron (9.33 mM) and nickel (0.93 mM) complexed with EDTA (10.3 mM). The different fungal isolates employed in this study showed a pickup of cobalt in the range 8-500 ng/g of dry biomass. The [Fe]/[Co] and [Ni]/[Co] ratios in the solutions before and after exposure to the fungi were also determined. At micromolar level the cobalt pickup by many fungi especially the mutants of N. crassa is seen to be proportional to the initial cobalt concentration taken in the solution. However, R. chinensis exhibits a low but iron concentration dependent cobalt pickup. Prior saturating the fungi with excess of iron during their growth showed the presence of selective cobalt pickup sites. The existence of cobalt specific sorption sites is shown by a model experiment with R. chinensis wherein at a constant cobalt concentration (0.034 {mu}M) and varying iron concentrations so as to yield [Fe/Co]{sub initial} ratios in solution of 10, 100, 1000 and 287 000 have all yielded a definite Co pickup capacity in the range 8-47 ng/g. The presence of Cr(III)EDTA (3 mM) in solution along with complexed Fe and Ni has not influenced the cobalt removal. The significant feature of this study is that even when cobalt is present in trace level (sub-micromolar) in a matrix of high concentration (millimolar levels) of iron, nickel and chromium, a situation typically encountered in spent decontamination solutions arising from stainless steel based primary systems of nuclear reactors, a number of fungi studied in this work showed a good sensitivity for cobalt pickup.

  14. Bioremediation of 60Co from simulated spent decontamination solutions

    International Nuclear Information System (INIS)

    Rashmi, K.; Naga Sowjanya, T.; Maruthi Mohan, P.; Balaji, V.; Venkateswaran, G.

    2004-01-01

    Bioremediation of 60 Co from simulated spent decontamination solutions by utilizing different biomass of (Neurospora crassa, Trichoderma viridae, Mucor recemosus, Rhizopus chinensis, Penicillium citrinum, Aspergillus niger and, Aspergillus flavus) fungi is reported. Various fungal species were screened to evaluate their potential for removing cobalt from very low concentrations (0.03-0.16 μM) in presence of a high background of iron (9.33 mM) and nickel (0.93 mM) complexed with EDTA (10.3 mM). The different fungal isolates employed in this study showed a pickup of cobalt in the range 8-500 ng/g of dry biomass. The [Fe]/[Co] and [Ni]/[Co] ratios in the solutions before and after exposure to the fungi were also determined. At micromolar level the cobalt pickup by many fungi especially the mutants of N. crassa is seen to be proportional to the initial cobalt concentration taken in the solution. However, R. chinensis exhibits a low but iron concentration dependent cobalt pickup. Prior saturating the fungi with excess of iron during their growth showed the presence of selective cobalt pickup sites. The existence of cobalt specific sorption sites is shown by a model experiment with R. chinensis wherein at a constant cobalt concentration (0.034 μM) and varying iron concentrations so as to yield [Fe/Co] initial ratios in solution of 10, 100, 1000 and 287 000 have all yielded a definite Co pickup capacity in the range 8-47 ng/g. The presence of Cr(III)EDTA (3 mM) in solution along with complexed Fe and Ni has not influenced the cobalt removal. The significant feature of this study is that even when cobalt is present in trace level (sub-micromolar) in a matrix of high concentration (millimolar levels) of iron, nickel and chromium, a situation typically encountered in spent decontamination solutions arising from stainless steel based primary systems of nuclear reactors, a number of fungi studied in this work showed a good sensitivity for cobalt pickup

  15. Establishing the estimation model on radiation level at the ambience of 60Co radiotherapy treatment room based on NCRP REPORT No.151

    International Nuclear Information System (INIS)

    Yang Haiyou; Liu Liping; Liang Yueqin; Yu Shui

    2009-01-01

    Objective: To establish the estimation model to evaluate the radiation level at the ambience of 60 Co radiotherapy treatment room. Methods: The estimation model derives from NCRP REPORT No.151- S tructural Shielding Design and Evaluation for Megavoltage X-and Gamma-Ray Radiotherapy Facilities b y making appropriate adjustment, which presents the calculation methods on radiation level at the ambience of megavoltage medical electron linear accelerator treatment room. Results: The application scope of estimation model from NCRP REPORT No.151 is extended to γ-radiotherapy facilities, and it can be regarded as a new model for calculating the radiation level at the ambience of 60 Co radiotherapy treatment room. Conclusion: The estimation model has certain reference value to evaluate the radiation level at the ambience of 60 Co radiotherapy treatment room. (authors)

  16. Biochemical and immunological alterations of {sup 60} Co irradiated Bothrops jararacussu venom; Alteracoes bioquimicas e imunologicas do veneno de Bothrops jararacussu irradiado com {sup 60} Co

    Energy Technology Data Exchange (ETDEWEB)

    Spencer, Patrick J.

    1995-12-31

    Proteins irradiation leads to structural alterations resulting in activity and function loss. This process has been useful to detoxify animal venoms and toxins, resulting in low toxicity products which increased immunogenicity. The Bothrops jararacussu venom behaves as a weak immunogen and its lethal activity in not neutralized by either autologous, heterologous or bothropic polyvalent antisera. This venom is markedly myotoxic and and the commercial bothropic antiserum does not neutralize this activity, because of this low immunogenicity of the myotoxins. This present work was done in order to evaluate the possibility of irradiating Bothrops jararacussu, intending to increase the immunogenicity of the myotoxic components, leading to productions of myotoxins neutralizing antibodies. Bothrops jararacussu venom samples were irradiated with 500, 1000 and 2000 Gy of {sup 60} Co gamma rays. A 2.3 folds decrease of toxicity was observed for the 1000 Gy irradiated samples while the 2000 Gy irradiated sample was at least 3.7 folds attenuated. On the other hand, the 500 Gy did not promote any detoxification. Electrophoresis and HPLC data indicate that the irradiation lead to the formation of high molecular weight products (aggregates). The proteolytic and phospholipasic activities decreased in a dose dependent manner, the phospholipases being more resistant than the proteases. Both the animals (rabbit) immunized with either native or 2000 Gy irradiated venom produced native venom binding antibodies, a slightly higher titer being obtained in the serum of the rabbit immunized with the irradiated samples. Western blot data indicate that the anti-irradiated venom Ig Gs recognised a greater amount of either autologous or heterologous venom bands, both sera behaving as genus specific. The anti-native serum did not neutralize the myotoxic activity of native venom, while the anti-irradiated one was able to neutralize this activity. (author). 56 refs., 7 figs.

  17. Effects of gamma radiation ({sup 60}Co) on the main physical and chemical properties of health care packaging and their compounds paper and multilayer plastic film, used for health products sterilization; Efeitos da radiacao gama (Cobalto-60) nas principais propriedades fisicas e quimicas da embalagens compostas por papel grau cirurgico e filme plastico laminado, destinadas a esterilizacao de produtos para saude

    Energy Technology Data Exchange (ETDEWEB)

    Porto, Karina Meschini Batista Geribello

    2013-07-01

    Gamma radiation is one of the technologies applied for the sterilization of packaging systems containing products for health. During sterilization process it is critical that the properties of packages are maintained. In this study two samples of commercial pouch packaging comprised of surgical grade paper on one side and the other side multilayer plastic film were irradiated with gamma rays. The following doses were applied 25 kGy (1,57 kGy/h) and 50 kGy (1,48 kGy/h). One packaging sample was paper formed by softwood fibers and multilayer plastic film based on poly(ethylene terephthalate) (PET)/polyethylene (PE). The second type of paper sample was made by a mixture of softwood and hardwood fibers and multilayer plastic film based on polyethylene terephthalate (ethylene) (PET)/polypropylene (PP). The effects of radiation on the physical and chemical properties of papers and multilayer plastic films, as well as the properties of the package were studied. The paper was the more radiation sensitive among the studied materials and radiation effects were more pronounced at brightness, pH, tearing resistance, bursting strength and tensile strength. Nonetheless, worst comparatively effects were noted on the sample made by a mixture of softwood and hardwood fibers. The porosity of paper was enhanced by 50 kGy. In the case of plastic films, radiation effects on tensile strength was the most pronounced property for both samples. In the case of the packaging the sealing resistance decreased with radiation. The effects observed for the treatment at 50 kGy were more pronounced when compared to 25 kGy. This last is the dose which is usually applied to sterilize health products. A dosimetry study was performed during irradiation at 25 kGy, 40 kGy and 50 kGy and its importance may be reported by the average dose variation 20 %. (author)

  18. Decommissioning of a 60Co unit and estimation of personal doses

    International Nuclear Information System (INIS)

    Lin, H.; Lin, P.; Liu, T.; Chu, C.

    2003-01-01

    Chang-hua Christian hospital needs to uninstall the 60 Co unit. The mode of this 60 Co teletherapy unit is SHIMADZU RTGS-10. The original lead head was taken as the source container of this 60 Co unit. The source head was dismantled and put into the prepared wooden box, after the source was sealed. This study describes the planning and dismantling of the retirement and transport of the 60 Co unit, and personal doses measured during the procedure. This work estimates the doses of radiation received by exposed workers during the dismantling of the machine. The workers received doses of approximately 53 μSv. This study shows that the original lead head can be used as the source container of this 60 Co unit. The 60 Co machine was smoothly dismantled and transported by conscientious and careful workers, using planned and controlled radiation protection, following the ALARA (as low as reasonably achievable) rule. (author)

  19. Bioaccumulation and elimination of 60 Co and 137 Cs by Anomalocardia brasiliana (Gmelin, 1791) (Mollusca bivalvia). Remobilization of 60 Co, retained in marine sediment by microbial activity

    International Nuclear Information System (INIS)

    Mayr, L.M.

    1984-05-01

    It was studied the capacity of a bi valve mollusc Anomalocardia brasiliana, which lives in the local bottom sediment, to remobilize 60 Co previously sorbed in the sediment. Laboratory experiments demonstrated that the transference of 60 Co from the sediment to the animal was insignificant (bioaccumulation factor (BF) of the order of 10 -3 ). At the same time, the capacity of microorganisms, present in the bottom sediment, to remobilize 60 Co was studied. The results showed that this via of transference was important, considering the much greater microorganism biomass in relation to the biomass of bentonic organisms, as a whole. For 137 Cs the determined BF from water to the animal was 2.2. and, as in the case of 60 Co, the soft tissues concentrated more 137 Cs than shell. Remaining viscera showed the highest BFs. In another series of experiments, the loss of 60 Co or 137 Cs, previously accumulated by A. brasiliana, was followed in aquaria with or without sediment and the respective biological half-lives were calculated. Soft tissues retained 60 Co longer (biological half-life = 117 days) than shells, whereas for 137 Cs the opposite was observed and shells showed a biological half life of 38.5 days. The low values of 60 Co and 137 Cs BF S do not allow to classify A. brasiliana as good biological indicator for pollution by there radionuclides. However since A brasiliana is consumed by the local population and is commercialized to other areas, it was recommended that its contamination by 60 Co or 137 Cs should be monitored. (author)

  20. Placental transfer of 60Co as a function of gestation age

    International Nuclear Information System (INIS)

    Zylicz, E.; Zablotna, R.; Szot, Z.

    1976-01-01

    The transfer of 60 Co from mother to foetus in relation to the time of gestation was examined 24 hrs after injecting 5 μCi of 60 CoCl 2 to the pregnant rat on 15th - 21st day of gestation. The radioactivity of foetuses, placentae as well as liver, kidney and femur of mother was determined. It was found that activity of 60 Co transferred to the foetus body increased with the time of gestation. (author)

  1. Studies on the transportation dynamics of 60Co in simulated ecosystem

    International Nuclear Information System (INIS)

    Wei Jianpeng; Chen Chuanqun; Wang Shouxiang; Sun Zhiming; Wang Jiyan

    1999-12-01

    The isotope tracer techniques were applied to study the transportation, accumulation and distribution of 60 Co in the pot-cultivated tomato-soil, aquatic and terrestrial ecosystems. Based on the principle of tracer dynamic compartment models, the mathematical formulae were established via computer simulation to describe the 60 Co behavior patterns in ecosystems and thus provided some basic information for elucidating the behavior of 60 Co in the environment. The results are as follows: (1) When 60 Co was introduced into the tomato-soil system, 60 Co was transported and accumulated in the soil and adsorbed by tomato root quickly, then transported to the above-ground plant. (2) The behavior patterns of 60 Co in the tomato-soil system could be described using the opened two-compartment model. (3) When 60 Co was introduced into aquatic system in the form of 60 Co-CoCl 2 , it was transported and transformed via deposit, complexation with other ions, adsorption and absorption by aquatic living things and led to the distribution and accumulation in individual part of the living things. (4) The behavior pattern of 60 Co in the aquatic-terrestrial ecosystem could be described by using opened five-compartment model

  2. Molecular exclusion chromatographic analysis on 60Co irradiated rattlesnake

    International Nuclear Information System (INIS)

    Murata, Y.; Rogero, J.R.

    1988-07-01

    Ionizing radiations are sufficiently energetic to be able of breaking chemical bonds and as result of that molecules of substances present in the irradiated will be chemically changed and their biological properties affected also. In this paper the effects of gamma radiation of Co-60 on the rattlesnake venom were studied. A pool of positive crotamina Crotalus durissus terrificus venom was dissolved in 0,15 M NaCl and irradiated with Co-60. Doses of 100, 250, 500, 750, 1500 and 2000 Gy were applied at the dose rate of 1190 Gy/h. With doses over 500 Gy the solutions became turbid, suggesting the presence of aggregates and structural changes of the proteins. The concentration of proteins from the filtered solution was measured by the Lowry method. One ml samples were measured at 230 nm. The partition coefficients and the areas of the three main fraction obtained were calculated. (author) [pt

  3. Evaluation by EPR of potential antioxidant components of 60Co-irradiated varieties of soybean

    International Nuclear Information System (INIS)

    Oliveira, Marcos Ronaldo Ramos de

    2009-01-01

    Brazil is today the second main producer of soybean in the world with a planted ground of about 21 million hectares and an annual production of 60 million tons in 2008, being slight more than a fourth of the annual production. The presence of flavonoids, particularly isoflavones in soybean products has been related as important for human health. It has been suggested that flavonoids play a role in the protection of plants by screening vital cellular components from damaging UV radiation. Electron paramagnetic resonance (EPR) spectroscopy can measure free radicals produced by dissociation molecules resulting from irradiation. It has been successfully employed for the detection of some irradiated food products. Twenty one Brazilian soybean cultivars from two crops were gamma-irradiated with a 60 Co source and evaluated by EPR. Correlation coefficients were made among the central EPR signal (g = 2.0039) and the total and partial isoflavones contents. There was no correlation with total contents, though glicitein and acetyl-daidzin showed a negative correlation. Even 7 months after irradiation the intensity of central EPR signal were high enough to distinguish the irradiated samples. EPR measurements of separated parts of the grain were more efficient for that purpose, particularly from hilum and coat. The radiation did not change substantially the total isoflavone contents, although there were some evidences suggesting some conversion of glycosides to aglycones. (author)

  4. Application of feedback system in optimizing safety performance of "6"0Co radiation apparatus

    International Nuclear Information System (INIS)

    Luo Shishi; Wang Zegang; Ge Cailin; Ma Fei; Gong Zheng

    2001-01-01

    To ensure "6"0Co apparatus runs safely applying the basic principle of cybernetics to optimizing safety performance was studied. Through several decades of practice the cybernetic system is shown to be safe and effective, and it will be an example for small and middle "6"0Co radiation apparatus to rebuild the cybernetic system. (authors)

  5. Detection and thermoluminescence of coriander (Coriandrum sativum L.) irradiated with 60Co

    International Nuclear Information System (INIS)

    Ruiz G, B.; Cruz Z, E.; Barboza F, M.

    2009-10-01

    The fresh and dried coriander (Coriandrum sativum L.) is a spice of high commercial value as food for consumption in Mexico. Their hygienic quality is often poor due to some factors: contaminants present in irrigation water and the cultivation atmosphere, harvesting and post harvest handling, mainly. The process by radiation is an alternative to achieve the sterilization to adequate dose for the hygienic quality for its consumption. However, irradiation also involves food detection exposed to radiation. This paper presents detected results on the dried coriander that was obtained from fresh samples and thermoluminescent properties such as glow curves structure from low doses (0.5 Gy) to high (15 kGy), the dose response, thermoluminescent signal decay, in order to determine the loss of stability during the storage of the poly mineral fraction. We obtained the inorganic fraction separation of the organic part of particle sizes of 10 μm by the Zimmerman method. The samples were exposed at two dose ranges; 0.5-400 Gy and 0.5-15 kGy of gamma radiation with 60 Co. The limit of thermoluminescent detection of the irradiated coriander samples was from 1 Gy. The glow curves were at a broad band of 35-400 C, with a maximum thermoluminescence around 182-196 C, and run at 164 C for high doses of 15 kGy. The range of linear response to dose was 4-25 Gy, whereas higher doses than the kGy order the response increase with the dose. The thermoluminescent properties of the coriander poly mineral fraction, show it can be used to identify irradiated food at gamma doses relatively low and even in doses of commercial interest due to the high stability of thermoluminescent signals. (Author)

  6. Whole-body retention of 60Co incorporated into a seaweed in rats

    International Nuclear Information System (INIS)

    Inaba, Jiro; Nishimura, Yoshikazu; Ichikawa, Ryushi

    1979-01-01

    The objective of this study is to compare whole-body retention in the rat of radiocobalt incorporated into a marine green alga with that of 60 Co in inorganic form. Ulva pertusa was incubated in aerated seawater containing 60 Co under fluorescent lamp for 7 days. The radioactive seaweed was homogenated and was given to rats via a stomach tube. The control group of rats was given 60 CoCl 2 with homogenate of non-radioactive seaweed. Whole-body retention of the radionuclide was determined by in vivo counting of the living animal. The result revealed that rats absorbed and retained much more 60 Co incorporated into the seaweed than 60 CoCl 2 . This fact should be taken into account in the estimation of internal dose due to radiocobalt released into marine environment. (author)

  7. Development and evaluation of a technique for in vivo monitoring of 60Co in the lungs

    International Nuclear Information System (INIS)

    Mello, J.Q. de; Lucena, E.A.; Dantas, A.L.A.; Dantas, B.M.

    2015-01-01

    60 Co is a fission product of 235 U and represents a risk of internal exposure of workers in nuclear power plants, especially those involved in the maintenance of potentially contaminated parts and equipment. The control of 60 Co intake by inhalation can be performed through in vivo monitoring. This work describes the evaluation of a technique through the minimum detectable activity and the corresponding minimum detectable effective doses, based on biokinetic and dosimetric models of 60 Co in the human body. The results allow to state that the technique is suitable either for monitoring of occupational exposures or evaluation of accidental intakes. (author)

  8. Development of technology for the large-scale preparation of 60Co polymer film source

    International Nuclear Information System (INIS)

    Udhayakumar, J.; Pardeshi, G.S.; Gandhi, Shymala S.; Chakravarty, Rubel; Kumar, Manoj; Dash, Ashutosh; Venkatesh, Meera

    2008-01-01

    60 Co sources (∼37 kBq) in the form of a thin film are widely used in position identification of perforation in offshore oil-well explorations. This paper describes the large-scale preparation of such sources using a radioactive polymer containing 60 Co. 60 Co was extracted into chloroform containing 8-hydroxyquinoline. The chloroform layer was mixed with polymethyl methacrylate (PMMA) polymer. A large film was prepared using the polymer solution containing the complex. The polymer film was then cut into circular sources, mounted on a source holder and supplied to various users

  9. Chain dechlorination of organic chlorinated compounds in alcohol solutions by 60Co gamma-rays, (1)

    International Nuclear Information System (INIS)

    Sawai, Takeshi; Shimokawa, Toshinari; Sawai, Teruko; Hosoda, Ieji; Kondo, Masaharu.

    1975-01-01

    A study was made on radiolytic dechlorination of pentachlorobenzene in alkaline alcohol solutions. The dechlorination yield (G(Cl - )) was found to depend on the alcohols used as solvent and the concentrations of the chlorinated benzene and hydroxide ion. The high yields obtained in alkaline 2-propanol, sec-butanol and ethanol indicate a chain process in the dechlorination reaction. The value of G(Cl - ) was highest in 2-propanol, and the principal products generated were potassium chloride, acetone and the lower chlorinated benzenes, while a decrease was seen in the hydroxide ion concentration. The concentrations produced of potassium chloride and acetone, as well as the decrease in hydroxide ion concentration, are all roughly equal at all doses. With increasing irradiation dose, pentachlorobenzene was dechlorinated to tetra, tri, di and monochlorobenzene. 1,2,4,5-tetrachlorobenzene, 1,2,4-trichlorobenzene and 1,4-dichlorobenzene were main products. A discussion is given of the detailed mechanism of the dechlorination in alkaline alcohols and the effect of alcohols on G(Cl - ). (auth.)

  10. The morphological changes of adult mouse testes after 60Co Gamma-radiation

    International Nuclear Information System (INIS)

    Koruji, M.; Movahedin, M.; Gourabi, H.; Jabbary Arfaee, A.

    2008-01-01

    Cytotoxic therapy can lead to prolonged azoospermia or even sterility. In the present study, we investigated the morphological changes of mouse testes after γ-Radiation. Methods: After anesthetizing of NMRI mice, testes and their surrounding tissues were irradiated using a cobalt therapy machine. Four experimental groups were irradiated with fractionated doses of: 1.5+8, 1.5+12 and 1.5+16 Gy (with an interval of 24 h) and single dose of 14 Gy. Non-irradiated mice were considered as control group. Testes were removed 4, 6 and 8 weeks following irradiation, weighed and processed for light microscopic study. Diameters of seminiferous tubules and their lumens, epithelium thickness, percentage of different types of tubules and number of spermatogenic cell were measured. Moreover, sperm count motility and viability rates were evaluated in epididymis. Results: Number of normal tubules, epithelium thickness, tubules diameter and lumen diameter were significantly reduced with high dose irradiation in comparison with control testes. The recovery was observed after 8 weeks. Epididymal sperm count, motility and viability rates were significantly decreased in the irradiated mice comparing non-irradiated ones. These parameters were increased after 8 weeks. Conclusion: According to the results, irradiation can cause temporary azoospermia in mouse and this effect is reversible after 8 weeks

  11. Roentgenographic observation on development and formation of the teeth irradiated by 60Co-gamma ray

    International Nuclear Information System (INIS)

    Kaneko, Masayuki; Chiba, Hiroshige; Akiyama, Akihiro; Kitamura, Nobuyasu; Iwamoto, Hidemitsu

    1978-01-01

    On a 4-year-old boy who received radiotherapy for malignant tumor in his right mandibula, development of the teeth of 3rd to the 7th in the upper left side and the 3rd to the 7th in the upper right side was observed for 8 years. In the teeth of the 3rd to the 7th in the upper left side which were in irradiated field, and the teeth, except for the teeth of the 3rd to the 6th in the upper left side which had already completed development before irradiation, showed remarkable abnormalities in the shape of the crown such as atrophy, dwarfishness, and flatness. Also the teeth of the 3rd to the 7th of the upper right side which were not in irradiated field, teeth except for the those of the 3rd to the 6th in the upper right side showed slight abnormality in the shape of the crown and root. In irradiated teeth of the 3rd to the 7th in the upper left side, development and formation of teeth were remarkably interfered so that the teeth were almost rootless. In non-irradiated teeth of the 3rd to the 7th in the upper right side atrophy, dwarfishness, and hypoplasia in width and in diameter too. In irradiated teeth of the 3rd to the 7th in the upper left side, calcification was highly advanced in comparison with that in non-irradiated teeth of the 3rd to the 7th in the upper right side or that in normal upper right side. Also in non-irradiated teeth of the 3rd in the 7th in the upper right side, calcification was slightly advanced compared with that in normal teeth. In irradiated teeth of the 3rd to the 7th in the upper left side, the eruption of the teeth was remarkably facilitated. The irradiated tooth of the 6th in the upper left side fell out early and the other teeth were seen in planted condition on the mandibula. This seemed to be due to the foreign body eliminating mechanism. (Ueda, J.)

  12. Measurements of integrated components' parameters versus irradiation doses gamma radiation (60Co) dosimetry-methodology-tests

    International Nuclear Information System (INIS)

    Fuan, J.

    1991-01-01

    This paper describes the methodology used for the irradiation of the integrated components and the measurements of their parameters, using Quality Insurance of dosimetry: - Measurement of the integrated dose using the competences of the Laboratoire Central des Industries Electriques (LCIE): - Measurement of irradiation dose versus source/component distance, using a calibrated equipment. - Use of ALANINE dosimeters, placed on the support of the irradiated components. - Assembly and polarization of components during the irradiations. Selection of the irradiator. - Measurement of the irradiated components's parameters, using the competences of the societies: - GenRad: GR130 tests equipement placed in the DEIN/SIR-CEN SACLAY. - Laboratoire Central des Industries Electriques (LCIE): GR125 tests equipment and this associated programmes test [fr

  13. Attempted sterilization of sexually undifferentiated fry of Tilapia zillii by 60Co gamma-ray irradiation

    International Nuclear Information System (INIS)

    Nelson, S.G.; Andersen, A.C.; Momeni, M.H.; Yeo, R.R.

    1976-01-01

    Attempts to lessen the potential environmental hazards of aquatic vegetation control by use of weed eating exotic fish which have been sterilized is discussed. It was found that the gonads of Tilapia are very resistant to radiation and this method is thus not practical

  14. Humoral immune response against native or {sup 60}Co irradiated venom and mucus from stingray Paratrygon aiereba

    Energy Technology Data Exchange (ETDEWEB)

    Thomazi, Gabriela Ortega Coelho; Alves, Glaucie Jussilane; Aires, Raquel da Silva; Turibio, Thompson de Oliveira; Rocha, Andre Moreira; Spencer, Patrick Jack; Nascimento, Nanci do, E-mail: 0916@prof.itpacporto.com.br [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil); Seibert, Carla Simone, E-mail: carlaseibert@yahoo.com [Universidade Federal do Tocantins (UFT), Porto Nacional, TO (Brazil)

    2015-07-01

    Poisonings and traumas caused by poisonous freshwater fish such as rays are considered a major public health problem and draw attention because of accidents involving these animals cause serious local symptoms and are disabling, keeping the victim away from work. The therapy of these cases is based only on the symptoms of patients, which implies in its low efficiency, causing suffering for the victims. This study aims to evaluate and compare the humoral immune response in animals inoculated with native or {sup 60}Co irradiated Paratrygon aiereba venom and mucus. Ionizing radiation has proven to be an excellent tool to decrease the toxicity of venoms and isolated toxins. The mucus and venom samples of P. aiereba were irradiated using gamma rays from a {sup 60}Co source. Animals models were immunized with the native or irradiated mucus or venom. The assays were conducted to assess the production of antibodies by the immunized animals using enzyme immunoassay and western blotting. Preliminary results show the production of antibodies by the immunized animals. The resulting sera were also checked for antigenic cross- reactivity between venom and mucus, demonstrating the potential of mucus as an antigen for serum production for the specific treatment for accidents by stingrays. However, it is essential to carry out further tests in order to verify the neutralization of the toxin by antibodies formed by animals. (author)

  15. Study by electron spin resonance (ESR) of 60 Co irradiated grains and farinaceous derivatives

    International Nuclear Information System (INIS)

    Catanni, Marta Mattos.

    1995-01-01

    The electron spin resonance (ESR) spectroscopy is being pointed out as one of the most promising techniques to determine whether a food has been irradiated. In this work, the ESR spectrum of paramagnetic radicals produced by gamma irradiation of grains and flour derivatives using a 60 Co source was investigated. Samples of grains (wheat and barley), flours (wheat, maniac, rye, soy bean and maize), bran and starch have been irradiated with doses between 0.2 and 70 KGy. It was shown that all varieties of grains and flours presented similar ESR spectra with variation in the free radicals signal intensity. Measurements at 9.5 and 34.5 GHz shown that spectra were composed probably by the superposition of four paramagnetic species with g-factors closed to that of the free electron (2 triplets, 1 doublet and 1 singlet). Hyperfine interactions and the correspondingly line widths were estimated through an spectrum computer simulation. It was established that ESR signal intensities increased with the irradiation dose for all samples. Up to the commercial admissible dose limit (1 kGy), the increase of ESR signal shown a linear behavior with the dose. The signal stability varied significantly with storage conditions and sample humidity. For 1 kGy-irradiated samples stored at room temperature and 14% humidity, the ESR radiation signals were possible to be detected up to 5 days after irradiation. When the same samples were stored at low temperatures (0 0 C) or freeze-dried (almost 0% humidity) the ESR signals were detected until 6 months after irradiation. Similar ESR spectrum found for grains and flours was obtained for diverse starchy food products. It was verified that it is possible to identify for a long time irradiated starchy foods when they are commercialized dehydrated or frozen. (author). 66 refs., 35 figs., 17 tabs

  16. Biochemical and immunological alterations of 60 Co irradiated Bothrops jararacussu venom

    International Nuclear Information System (INIS)

    Spencer, Patrick J.

    1995-01-01

    Proteins irradiation leads to structural alterations resulting in activity and function loss. This process has been useful to detoxify animal venoms and toxins, resulting in low toxicity products which increased immunogenicity. The Bothrops jararacussu venom behaves as a weak immunogen and its lethal activity in not neutralized by either autologous, heterologous or bothropic polyvalent antisera. This venom is markedly myotoxic and and the commercial bothropic antiserum does not neutralize this activity, because of this low immunogenicity of the myotoxins. This present work was done in order to evaluate the possibility of irradiating Bothrops jararacussu, intending to increase the immunogenicity of the myotoxic components, leading to productions of myotoxins neutralizing antibodies. Bothrops jararacussu venom samples were irradiated with 500, 1000 and 2000 Gy of 60 Co gamma rays. A 2.3 folds decrease of toxicity was observed for the 1000 Gy irradiated samples while the 2000 Gy irradiated sample was at least 3.7 folds attenuated. On the other hand, the 500 Gy did not promote any detoxification. Electrophoresis and HPLC data indicate that the irradiation lead to the formation of high molecular weight products (aggregates). The proteolytic and phospholipasic activities decreased in a dose dependent manner, the phospholipases being more resistant than the proteases. Both the animals (rabbit) immunized with either native or 2000 Gy irradiated venom produced native venom binding antibodies, a slightly higher titer being obtained in the serum of the rabbit immunized with the irradiated samples. Western blot data indicate that the anti-irradiated venom Ig Gs recognised a greater amount of either autologous or heterologous venom bands, both sera behaving as genus specific. The anti-native serum did not neutralize the myotoxic activity of native venom, while the anti-irradiated one was able to neutralize this activity. (author)

  17. 60Co γ-irradiation enhances expression of GAP-43 mRNA in rat brain

    International Nuclear Information System (INIS)

    Su Bingyin; Cai Wenqin; Zhang Chenggang

    2001-01-01

    Objective: To study the relationship between the expression of GAP-43 mRNA and nerve regeneration in rat brain after 60 Co γ-irradiation. Methods: Wistar rats were subjected to whole-body irradiation with 8 Gy 60 Co γ-rays. The expression of GAP-43 was detected by in situ hybridization histochemistry using Dig-cRNA probe. Results: It was found that the expression of GAP-43 mRNA increased in the cerebral cortex, caudate, putamen, globus pallidum, thalamus and hypothalamus one week after 8 Gy 60 Co γ-irradiation. The peak of GAP-43 mRNA expression was observed in the fourth week and then began to decrease but still remained at a higher than normal level. However, it decreased to a low level after 7 weeks. Conclusion: Enhanced expression of GAP-43 mRNA after 60 Co γ-irradiation in rat brain is associated with nerve regeneration and reconstruction of synapse

  18. Bioaccumulation and elimination of {sup 60} Co and {sup 137} Cs by Anomalocardia brasiliana (Gmelin, 1791) (Mollusca bivalvia). Remobilization of {sup 60} Co, retained in marine sediment by microbial activity; Bioacumulacao e eliminacao de {sup 60}Co e {sup 137}Cs por anomalocardia brasiliana (Gmelin, 1791) (Mollusca bivalvia). Remobilizacao de {sup 60}Co retido em sedimento marinho por atividade microbiana

    Energy Technology Data Exchange (ETDEWEB)

    Mayr, L M

    1984-05-01

    It was studied the capacity of a bi valve mollusc Anomalocardia brasiliana, which lives in the local bottom sediment, to remobilize {sup 60} Co previously sorbed in the sediment. Laboratory experiments demonstrated that the transference of {sup 60} Co from the sediment to the animal was insignificant (bioaccumulation factor (BF) of the order of 10{sup -3}). At the same time, the capacity of microorganisms, present in the bottom sediment, to remobilize {sup 60} Co was studied. The results showed that this via of transference was important, considering the much greater microorganism biomass in relation to the biomass of bentonic organisms, as a whole. For {sup 137} Cs the determined BF from water to the animal was 2.2. and, as in the case of {sup 60} Co, the soft tissues concentrated more {sup 137} Cs than shell. Remaining viscera showed the highest BFs. In another series of experiments, the loss of {sup 60} Co or {sup 137} Cs, previously accumulated by A. brasiliana, was followed in aquaria with or without sediment and the respective biological half-lives were calculated. Soft tissues retained {sup 60} Co longer (biological half-life = 117 days) than shells, whereas for {sup 137} Cs the opposite was observed and shells showed a biological half life of 38.5 days. The low values of {sup 60} Co and {sup 137} Cs BF{sub S} do not allow to classify A. brasiliana as good biological indicator for pollution by there radionuclides. However since A brasiliana is consumed by the local population and is commercialized to other areas, it was recommended that its contamination by {sup 60} Co or {sup 137} Cs should be monitored. (author).

  19. The Application of Fucus vesiculosus as a Bioindicator of 60Co Concentrations in the Danish Straits

    DEFF Research Database (Denmark)

    Boelskifte, S.

    1985-01-01

    The occurrence of 60Co in the Danish Straits is investigated by applying the seaweed Fucus vesiculosus as a bioindicator. In order to describe different dispersion situations, three areas have been studied separately: the North Sea, where it is possible to measure 60Co from sources in France and...... of distance from Barsebäck. Problems of uncertainty related to differences in environmental parameters are discussed and new investigations to improve the use of Fucus as a bioindicator are suggested....

  20. Analysis of the frequency of unstable chromosome aberrations in human lymphocytes irradiated with {sup 60}Co; Analise da frequencia de alteracoes cromossomicas instaveis em linfocitos humanos irradiados com {sup 60}Co

    Energy Technology Data Exchange (ETDEWEB)

    Mendonca, Julyanne C.G.; Mendes, Mariana E.; Lima, Fabiana F., E-mail: july_cgm@hotmail.com, E-mail: mendes_sb@hotmail.com [Centro Regional de Ciencias Nucleares (CRCN-NE/CNEN-PE), Recife, PE (Brazil); Santos, Neide, E-mail: santos_neide@yahoo.com.br [Universidade Federal de Pernambuco (CCB/UFPE), Recife, PE (Brazil). Departamento de Genetica

    2013-07-01

    The aim of this study was to analyze the frequency of unstable chromosomal aberrations induced by gamma radiation from a {sup 60}Co source at two different doses. Samples were obtained from a healthy donor and exposed to {sup 60}Co source (Gammacel 220 ) located in the Department of Nuclear Energy of Pernambuco Federal University (DEN/UFPe/Brazil) with a rate of air Kerma to 3,277 Gy/h. Exposures resulted in absorbed dose 0.51 Gy and 0.77 Gy. Mitotic metaphases were obtained by culturing lymphocytes for chromosome analysis and the slides were stained with 5% Giemsa. Among the unstable chromosomal aberrations the dicentric chromosomes, ring chromosomes and acentric fragments were analyzed. To calculate the significance level the chi - square test was used, considering relevant differences between the frequencies when the value of p < 0.05. To calculate the significance level of the chi - square test was used, considering relevant differences between the frequencies when the value of p < 0.05. The results showed that there was significant difference of the frequencies of dicentric chromosomes (from 0.18 to 0.51 to 0.37 Gy to 0.77 Gy), however there was no statistically significant difference between the frequencies of acentric fragments ( 0.054 to 0, 51 Gy to 0.063 to 0.77 Gy) and ring chromosomes (0.001 to 0.51 Gy to 0.003 to 0.77 Gy). The low number of rings is found justified, considering that in irradiated human lymphocytes, its appearance is rare relative to dicentrics. The results confirm that dicentrics are the most reliable biomarkers in estimating dose after exposure to gamma radiation. These two points will make the calibration curve dose-response being built for Biological Dosimetry Laboratory of CRCN-NE/CNEN.

  1. The effect of environmental factors on the uptake of 60Co by Paecilomyces catenlannulatus

    International Nuclear Information System (INIS)

    Fengbo Li; Xiaoyu Li; Lejin Fang; Zhimo Gao

    2014-01-01

    Paecilomyces catenlannulatus (P. catenlannulatus), a kind of important pathogenic fungi of forest pests, can be regarded as an excellent material to retard the fate and transport of radionuclides. The effect of environmental factors (i.e., pH, ionic strength, temperature and solution concentration) on the uptake of 60 Co by P. catenlannulatus was investigated by batch technique. The results showed that the uptake of 60 Co by P. catenlannulatus was independent of pH at pH 60 Co was observed with increasing pH from 5.0 to 8.0, then remained the high-level uptake at pH > 8.0 due to occurrence of precipitates. It is found that 60 Co uptake significantly decreased with increasing ionic strength at low pH, whereas little change was observed at high pH. The pH dependence adsorption indicates that the interaction mechanism between 60 Co and P. catenlannulatus is ion exchange at low pH and surface complexation at high pH conditions, respectively. Compared to Freundlich model, the adsorption isotherms can be fitted by Langmuir model very well. The thermodynamic data calculated from the temperature adsorption isotherms indicated that the uptake process of 60 Co by P. catenlannulatus was an endothermic and spontaneous process. This paper focused on potential application of P. catenlannulatus as suitable bio-materials for the preconcentration and removal of radionuclides from aqueous solutions in environmental pollution management. (author)

  2. Determination of 137Cs and 60Co pollution in the area of the Laguna Verde Nuclear Power Plant, Mexico

    International Nuclear Information System (INIS)

    Salas Mar, Bernardo

    2015-01-01

    The project 'Radiological Analysis of Environmental Samples in the Gulf of Mexico and the coast of Quintana Roo', had the aim of identifying and quantifying anthropogenic radionuclides in environmental samples consisting of silt, sand and sea water. This paper presents the results of the radiological analysis of these samples, which was made in the multichannel system for gamma spectrometry with hyper-pure germanium detector in the Laboratory of Radiological Analysis of Environmental Samples, located at the Physics Department, Faculty of Sciences, of the Autonomous National University of Mexico (UNAM). The sampled points are along the coast of the contiguous states of Tamaulipas, Veracruz, Tabasco, Campeche, Yucatan and Quintana Roo. This paper presents the qualitative and quantitative concentrations of the main identified anthropogenic radionuclides 60 Co and 137 Cs. (authors)

  3. Determination of 137Cs and 60Co pollution in the area of the Laguna Verde Nuclear Power Plant, Mexico.

    Science.gov (United States)

    Salas Mar, Bernardo

    2015-11-01

    The project 'Radiological Analysis of Environmental Samples in the Gulf of Mexico and the coast of Quintana Roo', had the aim of identifying and quantifying anthropogenic radionuclides in environmental samples consisting of silt, sand and sea water. This paper presents the results of the radiological analysis of these samples, which was made in the multichannel system for gamma spectrometry with hyperpure germanium detector in the Laboratory of Radiological Analysis of Environmental Samples, located at the Physics Department, Faculty of Sciences, of the Autonomous National University of Mexico (UNAM). The sampled points are along the coast of the contiguous states of Tamaulipas, Veracruz, Tabasco, Campeche, Yucatan and Quintana Roo. This paper presents the qualitative and quantitative concentrations of the main identified anthropogenic radionuclides (60)Co and (137)Cs. © The Author 2015. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  4. Diffusion of strongly sorbing cations (60Co and 152Eu) in compacted Febex bentonite

    International Nuclear Information System (INIS)

    Garcia-Gutierrez, M.; Missana, T.; Alonso, U.; Mingarro, M.; Cormenzana, J.L.

    2010-01-01

    tablets. The apparent diffusion coefficient (Da) can be obtained analysing the tracer concentration profile in the samples at the end of the experiment. FEBEX bentonite was compacted, at 1.65 g/cm 3 of dry density (the density considered in the Spanish reference concept for radioactive waste repositories in granite and clay), in cylindrical stainless-steel rings of 38 mm in diameter and 25 mm of length. A filter paper with the tracer was placed between two rings with the compacted clay and the system was closed with two end-pieces and two sintered filters. The diffusion cells were introduced in a vessel to avoid humidity loss. Synthetic water simulating the pore water composition at 1.65 g/cm 3 was used for bentonite saturation. Three experiments with each tracer ( 60 Co 1.0.10 -8 M and 152 Eu 3.9.10 -8 M) were performed. The experiments lasted 843 and 852 days, for 60 Co and 152 Eu respectively, time enough to obtain an evolved profile. At the end of the experiments, the cell is disassembled, the bentonite is sliced and the tracer activity is measured directly in each slice to obtain the tracer concentration profile within the compacted sample. As both tracers used are gamma emitters, a Packard Cobra II auto-gamma counter was used to measure the samples activity. The experimental results were modelled using the analytical solution for the instantaneous injection of a solute in a one-dimensional (1D) semi-infinite medium. A stochastic 1D model with GoldSim 9.60 computer code was additionally used to analyze the role of the paper filter in which the tracer was spiked in the calculations. The range of values for the apparent diffusion coefficients, D a , obtained with the analytical and numerical solution are D a (Co) = (1-3).10 -13 m 2 /s and D a (Eu) (1-2).10 -14 m 2 /s. Always the first slice fit better with the lower value. The filter paper or instantaneous plane source is therefore, a simple and useful method to determine apparent diffusion coefficients for sorbing

  5. Mechanisms of60Co uptake and loss by Scenedesmus obliquus and transfer to two benthic organisms

    International Nuclear Information System (INIS)

    Nucho, R.

    1989-10-01

    The affinity of Scenedesmus obliquus for 60 Co has been confirmed under various experimental conditions allowing for the age of cultures, the physiological state of the cells, the radioactivity level and stable cobalt concentration in the medium, and the acute or periodic nature of the contamination. The dry weight 60 Co concentration factor may reach 4x10 4 in LCO culture medium and is about 10 4 in natural water. 60 Co uptake by algae is a 95% passive phenomenon, initiated by radionuclide absorption on the cell walls and followed by intracellular diffusion in accordance with Fick's law. From the initial cationic form, radiocobalt develops anion forms at a rate proportional to the algal biomass, resulting in lower contamination of old and dense cultures. After transfer in natural or artificial nonradioctive medium, 60 Co is eliminated by Scenedesmus obliquus, mainly as Co 2+ ions, according to a two-phase exponential process with two biological half-lives. The study of radioactive cobalt transfer from algae to gammarids and to midge larvae reveals the extend to which phytoplankton predominate over water and sediment in contaminating these two organisms. The total 60 Co uptake depends significantly on whether the cells are incorporated in or deposited on the sediment. No biomagnification phenomenon of the radionuclide was found in any case, as the transfer factor was less than 1. Depuration of 60 Co by organisms involved a two-phase exponential process regardless of whether uptake occured directly or from food. The presence of sediment appears to reduce the 60 Co desorption rate, but from 85 to 95% of the initial quantity is eliminated. This percentage does not depend on the transfer pathway or the presence of organic matter, and indicates very slight assimilation of radiocobalt ingested with algal cells [fr

  6. Uptake and release of 54Mn and 60Co in Fucus vesiculosus L. and its epiphytes

    International Nuclear Information System (INIS)

    Carlson, Lena

    1990-01-01

    Fucus vesiculosus with epiphytic Pilayella littoralis was collected at a locality with low contamination of 54 Mn and 60 Co and transplanted at a locality in the vicinity of the Barsebaeck nuclear power plant in the Oeresund, southern Sweden and vice versa. The highest uptake of the radionuclides discharged from the nuclear power plant was observed in the filamentous epiphytic P. littoralis and difference in uptake of 60 Co was also observed between tissues of different ages in F. vesiculosus, with slightly higher uptake in the new vegetative fronds. P. Littoralis was succeded as epiphyte by Enteromorpha intestinalis and Cladophora sp. and the highest uptake of both radionuclides was observed in Cladophora sp. Compared with the native Fucus plants at Barsebaeck, the transplanted Fucus plants showed about the same activity concentrations of 54 Mn while the 60 Co concentrations in the transplanted plants were higher than in the native plants after about four months. The release of 54 Mn and 60 Co was most rapid in the epiphytic P. littoralis. Biological half-lives calculated from an exponential relation were found to be about 10 days for p. littoralis and about 40 days for new vegetative fronds of F vesiculosus. Longer half-lives were found for 1-year-old tissues of F. vesiculosus: about 95 and 165 days for 54 Mn and 60 Co, respectively. One year after transplantation about 30% of the initial activity concentration of 60 Co was still present in the older part of the F. vesiculosus plants. The differences in uptake of 54 Mn and 60 Co between the algal species illustrates the difficulties encountered in comparing results from different algal species. (author)

  7. Effect of cyanide on the distribution of 60Co in mice

    International Nuclear Information System (INIS)

    Frankenberg, L.

    1982-01-01

    The effect of cyanide on the distribution of cobalt was studied by injecting mice i.p. with potassium cyanide 61.4 μmoles/kg, followed 1 min later by 60 CoCl 2 38.5 μmoles/kg i.v. Blood and tissue concentrations of 60 Co were then determined up to 48 h after injection and the results were compared with those obtained from control animals receiving 60 CoCl 2 only. In animals treated with cobalt plus cyanide, considerably higher concentrations of 60 Co were found in the spleen in comparison with controls during the whole observation period, whereas the 60 Co concentration in the pancreas was lower than that of controls during the first 4 h. In other tissues no significant differences between cyanide-treated animals and controls were noticed. Determination of complex-bound cyanide demonstrated that the increased uptake of cobalt in the spleen observed in cyanide-treated animals was due to cobalt-cyanide complex(es). Cyanide did not affect the plasma protein binding of cobalt. (orig.)

  8. Genomic instability induced by 60Co γ ray radiation in normal human liver cells

    International Nuclear Information System (INIS)

    Gen Xiaohua; Guo Xianhua; Zuo Yahui; Wang Xiaoli; Wang Zhongwen

    2007-01-01

    Objective: To explore the genomic instability induced by 60 Co γ rays. Methods: The cloning efficiency and micronucleus efficiency of normal human liver cell irradiated by 60 Co γ rays were detected, and the method of single cell gel electrophoresis (SCGE) was carried out to measure DNA chains damage. The fast-growing cells were divided into different dose-groups and then irradiated by 60 Co γ rays. After 40 populations doubling, the progenies were secondly irradiated with 2 Gy 60 Co γ rays. Results: The cloning efficiency decreased with the increase of doses after the initial irradiation. After the survival cells were given second irradiation, both results of SCGE and micronucleus frequency showed that the second damage was correlated with the original irradiation doses. Conclusions: 60 Co γ rays can not only induce the immediate biological effects in liver cells, but also lead to the genomic instability in the descendants that leads to an enhanced frequency of genetic changes occurring among the progeny of the original irradiated cell. The expanding effect of second event helps to study the genomic instability. (authors)

  9. Relative contributions of food and water in the accumulation of 60Co by a freshwater fish

    International Nuclear Information System (INIS)

    Baudin, J.P.; Fritsch, A.F.

    1989-01-01

    Because of its presence in the natural environment a laboratory research program was undertaken with 60 Co, focused on a freshwater fish, Cyprinus carpio. In this paper results from laboratory experiments conducted to assess the role of food and water in the uptake and retention of 60 Co by the carp are reported. The 60 Co accumulation by the fish from water, from food and from both sources simultaneously were studied during the first phase. After this exposure phase the individuals are transferred to non-radioactive water and/or received unlabelled food to follow depuration of the radionuclide. There was no significant difference between the fish of the 3 treatment groups; the greatest fraction of residual radiocobalt resided in digestive tract that accounted for 20-25% of the total 69 Co body burden. (author)

  10. Mutagenesis of the somaclones in vitro of cut roses by 60Co γ-rays irradiation

    International Nuclear Information System (INIS)

    Qu Suping; Su Yan; Wang Lihua; Tang Kaixue; Wang Jihua; Zhang Hao

    2009-01-01

    Mutagenesis of cut rose in vitro irradiated by 60 Co γ-rays was studied. The callus of leaves and regenerations adventitious bud was used as the explants for mutagenesis. Effect of 60 Co γ-rays irradiation on the callus's regeneration rate, adventitious bud's multiplication rate and vegetal status were studied. The results showed that the regeneration frequency of callus was decreased by 60 Co γ-rays irradiation. The regenerations adventitious bud was the better experimental materials compared with the callus of leaves. The lethal dose was 122 Gy and the semi-lethal dose was 76 Gy according to the regression equation. The appropriate dose on adventitious bud by irradiation rays was 50-60 Gy. (authors)

  11. The effect of 60Co γ irradiation on culture in vitro of Pogostemon cablin (blanco) benth

    International Nuclear Information System (INIS)

    Wu Lirong; He Hong; Zhang Yanling; Liu Xing; Lin Xiaohua

    2008-01-01

    The 60 Co γ-irradiation on culture in vitro of Pogostemon cablin is studied to breed new variety. The explants used for 60 Co γ-irradiation were the leaf segments, nodular stem segments, stem segments and root tips from Pogostemon cablin. The media for culture in vitro were MT basal media containing 0.05mg/L BA. As irradiation dosage increased, the mortality of different explants from Pogostemon cablin increased. The irradiation dosage of LD50 for leaf segments, nodular stem segments and stem segments were 72, 64 and 66Gy, respectively, which were deduced from the regression equation. As the irradiation dosage increased, the ability of the plant regeneration of the explants decreased. 60 Co γ-irradiation could make the explants damage or die. Irradiation could inhibit the regeneration of explants from Pogostemon cablin obviously. The changes in the appearance of the regenerated plants were also observed. (authors)

  12. Effects of 60Co γ-ray irradiation on human platelets

    International Nuclear Information System (INIS)

    Wu Guoxin; Zhao Yiming; Ruan Changgeng

    1991-02-01

    Human platelet-rich plasma was irradiated with various doses (0,1.25,2.5,5.0,10,20,40Gy) of 60 Co γ-ray so as to observing the changes of metabolites and releasing substances of platelets. Alpha-granule membrane protein (GMP-140) molecules on the surface of platelet was expressed significantly increasing when the dosage of 60 Co γ-ray was 5 Gy; however, the glycoprotein (GP) I b and III a was not changed significantly; thromboxane B 2 production in plasma was significantly elevated while the γ-ray was only 2.5 Gy; the concentration of von Willebrand factor was increased when the γ-ray was over 5 Gy, this is in accordance with the GMP-140 molecules. These results indicate that platelets could be activated in vitro when the dosage of 60 Co γ was over 5 Gy

  13. 60Co, 63Ni and 94Nb soil-to-plant transfer in pot experiments

    International Nuclear Information System (INIS)

    Gerzabek, M.H.; Mohamad, S.A.; Mueck, K.; Horak, O.

    1995-01-01

    Soil-to-plant transfer factors for 60 Co, 63 Ni and 94 Nb were obtained via pot experiments with a Dystric Cambisol and a Calcic Chernozem, both from Lower Austria. Investigated plants were greenrape (Brassica napus oleifera L.), bean (Phaseolus vulgaris L.) and winter wheat (Triticum aestivum L.). The soil-to-plant transfer factors decreased from 63 Ni to 60 Co and 94 Nb. Mean values from all experiments ranged from 1.12 ( 63 Ni) to 0.0045 ( 94 Nb). The transfer values obtained for 60 Co and 63 Ni are comparable to literature values, but 94 Nb-transfer seems to be lower than previous estimates. All radionuclides showed differences between plant species and plant organs. Transfer values were also dependent on the soil type. (author)

  14. Shear Stress in Nickel and Ni-60Co under One-Dimensional Shock Loading

    International Nuclear Information System (INIS)

    Workman, A.; Wallwork, A.; Meziere, Y. J. E.; Millett, J. C. F.; Bourne, N. K.

    2006-01-01

    The dynamic response of pure nickel (Ni), and its alloy, Ni-60Co (by weight %), has been investigated during one-dimensional shock loading. Few materials' properties are different and the only significantly altered feature is the reduced stacking fault energy (SFE) for the Ni-60Co. This paper considers the effect of this reduced SFE on the shear strength. Data (in terms of shock stress, particle velocity and shock velocity) are also presented. The influence on the shear stress, τ of cobalt additions in nickel are then investigated and presented. Results indicate that the lateral stress is increasing in both materials with the increasing impact stress. The shear stress was found to be higher in the nickel than in the Ni-60Co. The progressive decrease of the lateral stress noted during loading indicates a complex mechanism of deformation behind the shock front

  15. Validation of dose-response curve of CRCN-NE - Regional Center for Nuclear Sciences from Northeast Brazil for {sup 60}Co: preliminary results; Validacao da curva dose-resposta do CRCN-NE para {sup 60}Co: resultados preliminares

    Energy Technology Data Exchange (ETDEWEB)

    Mendonca, Julyanne C.G.; Mendes, Mariana E.; Hwang, Suy F.; Lima, Fabiana F. [Centro Regional de Ciencias Nucleares do Nordeste (CRCN-NE/CNEN-PE), Recife, PE (Brazil); Santos, Neide, E-mail: july_cgm@yahoo.com.br [Universidade Federal de Pernambuco (CCB/UFPE), Recife, PE (Brazil). Departamento de Genetica

    2014-07-01

    The cytogenetic study has the chromosomal alterations as biomarkers in absorbed dose estimation by the body of individuals involved in exposure to ionizing radiation by interpreting a dose response calibration curve. Since the development of the technique to the analysis of data, you can see protocol characteristics, leading the International Atomic Energy Agency indicate that any laboratory with intention to carry out biological dosimetry establish their own calibration curves. The Biological Dosimetry Laboratory of the Centro Regional de Ciencias Nucleares (CRCN-NE/CNEN), Brazil, recently established the calibration curve related to gamma radiation ({sup 60}Co). Thus, this work aimed to start the validation of this calibration curve from samples of three different blood donors which were irradiated with an absorbed known single dose of 1 Gy. Samples were exposed to {sup 60}Co source (Glaucoma 220) located in the Department of Nuclear Energy (DEN/UFPE). After fixation with methanol and acetic acid and 5% Giemsa staining, the frequency of chromosomal alterations (dicentric chromosomes, acentric rings and fragments) were established from reading of 500 metaphases per sample and doses were estimated using Dose Estimate program. The results showed that, using the dose-response curve calibration for dicentrics, the dose absorbed estimated for the three individuals ranged from 0.891 - 1,089Gy, taking into account the range of confidence of 95%. By using the dose-response curve for dicentrics added to rings and for the same interval of confidence the doses ranged from 0,849 - 1,081Gy. Thus, the estimative encompassed known absorbed dose the three individuals in confidence interval of 95%. These preliminary results seems to demonstrate that dicentric dose-response curves and dicentrics plus rings established by CRCN-NE / CNEN are valid for dose estimation in exposed individuals. This validation will continue with samples from different individuals at different doses.

  16. Investigation of Chitosan for Decorporation of 60Co in the Rat

    International Nuclear Information System (INIS)

    Levitskaia, Tatiana G.; Creim, Jeffrey A.; Curry, Terry L.; Luders, Teresa; Morris, James E.; Sinkov, Sergey I.; Woodstock, Angela D.; Thrall, Karla D.

    2009-01-01

    Purpose: The reported investigation is a part of our on-going research aimed at identifying effective in vivo non-toxic decorporation agents and developing new therapies to treat internal contamination with radionuclides. The non-toxic nature of chitosan makes it an especially attractive candidate for unsupervised treatment of the general population in case of radiological/nuclear emergency. In this study, chemically unmodified water-soluble chitosan oligosaccharide of low molecular weight was tested for decorporation of cobalt-60 (Co-60) using a rodent model. Methods: Affinity of chitosan oligosaccharide for Co(II) was tested in vitro under conditions of physiological pH range and ionic strength using combined spectrophotometric and potentiometric titration techniques. Fisher F344 rat model was used for in vivo studies. To evaluate effect of chitosan on ingested Co-60, animals received single oral dose of Co-60 chloride (7-13.2 kBq per animal) followed by oral administration of chitosan material (288-366 mg per kg body weight); chitosan dosing was repeated in 24 hours. Chitosan was also tested for removal of internalized Co-60. In this study, Co-60 single intravenous injection (7-8 kBq per animal) was followed by repetitive oral (300 mg per kg body weight) or intravenous (195 mg per kg body weight) administration of the chitosan material once daily for 5 days. Control animal groups received a single dose of Co-60 without chelator treatment. Excreta was collected daily. Tissues were collected postmortem and analyzed for radioactivity by gamma counting technique. Results: In vitro experiments confirmed binding of Co(II) by chitosan oligosaccharide, formation of mixed cobalt-chitosan-hydroxide complex species was proposed, and stability constants was calculated. Control in vivo studies indicated that about 71% of ingested Co-60 was excreted in two days predominantly through the gastrointestinal tract. For intravenously administered Co-60, urinal excretion was

  17. Experimental studies on the influence of irradiation (60Co) on wound healing of colonic suture

    International Nuclear Information System (INIS)

    Shoji, Munehiro

    1980-01-01

    Effects of preoperative 60 Co irradiation on wound healing of colonic suture in adult dogs of mixed breed were discussed. Wound healing 7 days after colonic suture was compared among dogs which received colonic suture immediately, or 3 days, or 7 days after the 60 Co irradiation. As a result, there was not a big difference in their wound healing among these dogs, and disorders of wound healing were not found. Bursting pressure at sutured sites was always lower in irradiated dogs than controls. Recovery of the bursting pressure was 2 days slower in irradiated dogs than non-irradiated dogs for 7 days after the suture. Bursting pressure was the lowest on the 3rd day after the suture in both groups. As to fine changes in irradiated dogs, appearance of recommunicating phenomena in blood vessels, delay of a decrease in avascular regions, delay of granulation, and hypertrophy of vascular walls seemed to be influenced by 60 Co irradiation, but there was not a big difference in these results between irradiated and non-irradiated dogs. An increase in postoperative concentration of hydroproline tended to be little lower in irradiated dogs than controls, which seemed to be influenced by the 60 Co irradiation. This result was also suggested from delay of granulation and remaining inflammations. Effects of 60 Co irradiation on wound healing of colonic suture seemed to be slight generally, because ruptured suture scarecely occurred in the irradiated dogs. When doses used in this experiment were calculated roughly by Ellis's method, irradiation of 60 Co seemed not to influence seriously wound healing if the total of 2,000 to 2,500 rad was irradiated in 8 to 10 doses to human colon. (Tsunoda, M.)

  18. Radiation effects of protons and 60Co γ rays on CMOS operational amplifier

    International Nuclear Information System (INIS)

    Lu Wu; Ren Diyuan; Guo Qi; Yu Xuefeng; Yan Rongliang

    1997-01-01

    Radiation effects of 60 Co γ ray and 4,7 and 30 MeV protons on LF 7650 CMOS operational amplifier were investigated. The damage mechanism of LF7650 was discussed. It is indicated that the mobility reduction of major carrier caused by ionizing and displacement damage is the chief mechanism causing the failure of CMOS operational amplifier irradiated by protons, and that is why the degradation of LF 7650 caused by protons is much more serious than that caused by 60 Co γ ray. In addition, a comparison of proton radiation effects on CMOS operational amplifier and MOSFET showed a significant difference in mechanism

  19. Uptake and release of 54Mn and 60Co in Fucus vesiculosus L. and its epiphytes

    International Nuclear Information System (INIS)

    Carlson, L.

    1994-01-01

    Fucus vesiculosus L. with epiphytic Pilayella littoralis (L.) Kjelm, Enteromorpha intestinalis (L.) Link and Cladophora sp, was collected at a swedish locality where contamination by 54 Mn and 60 Co was relatively low and then transplanted to a locality in the vicinity of the Barsebaeck nuclear power plant in the Oeresund, Southern Sweden. The reverse process was also carried out. Differences in uptake and release of 54 Mn and 60 Co were studied in the various species and in tissues of different ages. (Author)

  20. Coefficients of leaf-fruit translocation for 60Co, 90Sr and 137Cs in bean plant (Phaseolus vulgaris)

    International Nuclear Information System (INIS)

    Macacini, Jose Flavio

    2000-01-01

    Due to the increasing use of nuclear fission for the generation of electrical energy, the safety aspects of power plants must be minutely appraised. In case of an accident, with liberation of radioactive material into the atmosphere, knowledge about the behavior of plant species when in contact with radionuclides is indispensable. An important route through which agricultural products are contaminated by radionuclides is leaf-fruit translocation. This phenomenon can be evaluated by simulating a fallout contamination in a controlled atmosphere using as a tracer man-made radionuclides. In order to quantity the leaf-fruit translocation coefficients for 60 Co, 90 Sr and 137 Cs in the common bean (Phaseolus vulgaris), variety black diamond, an experiment was carried out in a greenhouse with completely randomized blocks design with six treatments and four blocks. A mixture of these three radionuclides was prepared and used to determine their translocation coefficients. The bean plants were contaminated inside a device especially designed to avoid environmental contamination. In each treatment four vases were sprinkled and one was used to estimate the initial activity of the other three vases. High-resolution gamma-ray spectrometry was used for 60 Co and 137 Cs activity determinations and chemical separation followed by beta counting of 90 Y was used for 90 Sr determinations. The number of treatments was reduced from six to four sprayings corresponding to 30, 45, 60 and 75 days after planting. This reduction was due to the attack of common and gold mosaic viroses. Symptoms were observed on the diseased bean plants 50 days after planting. It was possible, however, to verify a functional dependence between instant of tracer application and the level of physiological development of the bean plant. It was verified that the temporal relationship values for leaf-fruit translocation were similar for 60 Co and 137 Cs. For the 90 Sr, the translocation was below 2,5 mBq kg -1 /Bq

  1. Comparative analysis of {sup 60}Co intensity-modulated radiation therapy

    Energy Technology Data Exchange (ETDEWEB)

    Fox, Christopher [Sun Nuclear Corporation, 425-A Pineda Court, Melbourne, FL 32940 (United States); Romeijn, H Edwin; Lynch, Bart; Dempsey, James F [Department of Radiation Oncology, University of Florida, Gainesville, FL 32610-0385 (United States); Men, Chunhua [Department of Industrial and Systems Engineering, University of Florida, Gainesville, FL 32611-6595 (United States); Aleman, Dionne M [Department of Mechanical and Industrial Engineering, University of Toronto, Toronto, Ontario (Canada)], E-mail: chrisfox@sunnuclear.com, E-mail: romeijn@ise.ufl.edu, E-mail: lynchb@ufl.edu, E-mail: chhmen@ufl.edu, E-mail: aleman@mie.utoronto.edu, E-mail: dempsey@ufl.edu

    2008-06-21

    In this study, we perform a scientific comparative analysis of using {sup 60}Co beams in intensity-modulated radiation therapy (IMRT). In particular, we evaluate the treatment plan quality obtained with (i) 6 MV, 18 MV and {sup 60}Co IMRT; (ii) different numbers of static multileaf collimator (MLC) delivered {sup 60}Co beams and (iii) a helical tomotherapy {sup 60}Co beam geometry. We employ a convex fluence map optimization (FMO) model, which allows for the comparison of plan quality between different beam energies and configurations for a given case. A total of 25 clinical patient cases that each contain volumetric CT studies, primary and secondary delineated targets, and contoured structures were studied: 5 head-and-neck (H and N), 5 prostate, 5 central nervous system (CNS), 5 breast and 5 lung cases. The DICOM plan data were anonymized and exported to the University of Florida optimized radiation therapy (UFORT) treatment planning system. The FMO problem was solved for each case for 5-71 equidistant beams as well as a helical geometry for H and N, prostate, CNS and lung cases, and for 3-7 equidistant beams in the upper hemisphere for breast cases, all with 6 MV, 18 MV and {sup 60}Co dose models. In all cases, 95% of the target volumes received at least the prescribed dose with clinical sparing criteria for critical organs being met for all structures that were not wholly or partially contained within the target volume. Improvements in critical organ sparing were found with an increasing number of equidistant {sup 60}Co beams, yet were marginal above 9 beams for H and N, prostate, CNS and lung. Breast cases produced similar plans for 3-7 beams. A helical {sup 60}Co beam geometry achieved similar plan quality as static plans with 11 equidistant {sup 60}Co beams. Furthermore, 18 MV plans were initially found not to provide the same target coverage as 6 MV and {sup 60}Co plans; however, adjusting the trade-offs in the optimization model allowed equivalent target

  2. Screening of mutant strains producing phytase from A. niger by 60Co γ-ray irradiation

    International Nuclear Information System (INIS)

    Yang Pingping; Wang Yan; Tao Wenyi

    2004-01-01

    60 Co γ-ray was used to irradiate Aspergillus niger 447-92 for screening the mutant strain of producing phytase, and the effects of mutation induction were determined and analyzed. A mutant strain A. niger 496-1 with high level of phytase was selected, the phytase properties of A. niger 496-1 were analyzed

  3. Calculation of radiation production of high specific activity isotopes 192Ir and 60Co

    International Nuclear Information System (INIS)

    Zhou Quan; Zhong Wenfa; Xu Xiaolin

    1997-01-01

    The high specific activity isotopes: 192 Ir and 60 Co in the high neutron flux reactor are calculated with the method of reactor physics. The results of calculation are analyzed in two aspects: the production of isotopes and the influence to parameters of the reactor, and hence a better case is proposed as a reference to the production

  4. Studies on bone marrow damages after 60Co irradiation using uncalcified method

    International Nuclear Information System (INIS)

    Yamada, Mitsuaki

    1976-01-01

    Acute bone marrow degeneration and early regeneration after local 60 Co irradiation to rat bone marrow were studied histologically with the use of a ''Cut-all microtome''. With the use of Epon embedding, this method makes it possible to observe bone marrow in the natural state, especially to observe sinusoidal and stromal changes. After 60 Co irradiation of 500 and 1000 r to rat bone marrow, degeneration and disappearance of hematopoietic elements of the erythropoietic and granulopoietic series were noted within three days. In the hematopoietic elements of the megakaryocytic series, after 60 Co irradiation of 500 r, only mild changes were found, but after 60 Co irradiation of 1000 r, significant changes were noted. Sinusoidal and stromal reaction was also noted. Hematopoietic depression and regeneration were correlated with the disappearance and regeneration of the sinusoidal microcirculation. Against the previous reports, in the non-irradiated bone marrow, mild degeneration of the sinusoid was noted. In this study, associated with the degeneration of sinusoid -dilatation of the sinusoid and exudation-, disappearance of hematopoietic cells was noted. The etiology of the above fact is not know at present. (Evans, J.)

  5. Standardization of the measurement of the dose of 60Co teletherapeutic radiation

    International Nuclear Information System (INIS)

    Li Jinyu

    1998-01-01

    Present situation of radiatherapy for cancer with 60 Co γ-ray is narrated. An urgent need to carry out an unified and easily exactable code of practice in radiatherapy dosimetry is put forward, and the method for measuring the absorbed dose to water in an water phantom at the calibration point is introduced according to IAEA TRS No. 277

  6. Mutant in tobacco anther culture induced by 60Co γ-rays

    International Nuclear Information System (INIS)

    Tong Daoru; Jia Xinghua

    1991-01-01

    The tobacco anthers at uninucleate eccentric stage were irradiated by 60 Co γ-rays for the purpose of inducing desirable mutants. The results showed that the induction frequency of plantlets increased following 1kR of 60 Co γ-rays treatment. However, the time of plantlet induction was delayed and the percentage of responding anthers as well as the number of plantlets induced per anther significantly decreased after 3kR of 60 Co γ-ray radiation which was considered as a semilethal exposure. The plantlet numbers induced per anther were extremely low following 6kR of 60 Co γ-ray radiation. A white flower mutant appeared in the induced progenies. The tobacco leaf quality of this mutant were significantly improved as compared with its parental line. The mutant line has been tested and proved to have commercial value though the resistance to the black shank of tobacco slightly decreased as compared with the parental line

  7. Effect of 60Co γ-rays irradiation on rice straw fibre structure and enzyme hydrolyzation

    International Nuclear Information System (INIS)

    Chen Jingping; Li Wenge; Peng Ling; Wang Keqin; Xiong Xingyao

    2008-01-01

    The effect of improving enzyme hydrolyze of rice straw was estimated with treating dry rice straw and raw fiber by 60 Co γ-rays irradiation. the water-soluble deoxidize carbohydrate and total carbohydrate of 60 Co γ-rays irradiated rice straw and raw fibres were measured by DNS method and vitrol-phenol method. The changes of deoxidize carbohydrate groups of irradiated hydrolyzing rice straw were analyzed by gas chromatography. The organism structures of irradiated rice straw were scanned by electron microscope, the results showed that 1000-1500 kGy 60 Co γ-irradiation doses effectively destroyed rice straw's organism structures, especially the silicon crystal structures, and along with irradiation doses increased the breakage degree enlarged significantly. The contents of the water-soluble deoxidize carbohydrate and total carbohydrate of rice straw increased significantly. treated by both irradiation and enzyme, the cellulose transform rate of rice straw was 88.7%, which is better than that only treated by 60 Co γ-irradiation or enzyme. The content of water-solubility deoxidize carbohydrate of the treated rice straw was 214.4 mg/g and the total carbohydrate of straw was 758.5 mg/g. The contents of mannose, galactose, glucose, arabinose and xylose increased significantly, among those carbohydrate, the glucose's increment was the largest and account for 62.64%, and mannose's increments was the second. The contents of lignin of the rice straw were not influenced obviously by irradiation treatment. (authors)

  8. Effect of 60Co γ-ray irradiation on salmonella in high immunity egg yolk

    International Nuclear Information System (INIS)

    Ma Haili; Han Keguang; Zheng Mingxue; Li Guozhu

    2002-01-01

    Salmonella were inoculated into the yolk and irradiated with different dose of 60 Co γ-ray. The results show that 3 kGy irradiation dose can kill all Salmonella in the yolk and D 10 value of Salmonella in the yolk is 0.36-0.46 kGy

  9. Leaching of 60 Co and 137 Cs from spent ion exchange resins in ...

    Indian Academy of Sciences (India)

    Cement; radioactive waste; composite; waste management. Abstract. The leaching rate of 60Co and 137Cs from the spent cation exchange resins in cement–bentonite matrix has been studied. The solidification matrix was a standard Portland cement mixed with 290–350 (kg/m3) spent cation exchange resins, with or ...

  10. Comparison of the standards of absorbed dose to water of the VNIIFTRI, Russia and the BIPM for 60Co γ rays

    International Nuclear Information System (INIS)

    Allisy-Roberts, P.J.; Burns, D.T.; Berlyand, V.; Bregadze, Y.; Korostin, S.

    2003-09-01

    A comparison of the standards of absorbed dose to water of the All-Russian Scientific Research Institute for Physical-Technical and Radio-technical Measurements (VNIIFTRI), Russia and of the Bureau International des Poids et Mesures (BIPM) has been made in 60 Co gamma radiation. The results show that the VNIIFTRI and the BIPM standards for absorbed dose to water are in agreement, yielding a mean ratio of 0.9967 for the calibration factors of the transfer chambers, the difference from unity being within the combined standard uncertainty (0.0043) for this result. (authors)

  11. A comparison of large-scale electron beam and bench-scale 60Co irradiations of simulated aqueous waste streams

    Science.gov (United States)

    Kurucz, Charles N.; Waite, Thomas D.; Otaño, Suzana E.; Cooper, William J.; Nickelsen, Michael G.

    2002-11-01

    The effectiveness of using high energy electron beam irradiation for the removal of toxic organic chemicals from water and wastewater has been demonstrated by commercial-scale experiments conducted at the Electron Beam Research Facility (EBRF) located in Miami, Florida and elsewhere. The EBRF treats various waste and water streams up to 450 l min -1 (120 gal min -1) with doses up to 8 kilogray (kGy). Many experiments have been conducted by injecting toxic organic compounds into various plant feed streams and measuring the concentrations of compound(s) before and after exposure to the electron beam at various doses. Extensive experimentation has also been performed by dissolving selected chemicals in 22,700 l (6000 gal) tank trucks of potable water to simulate contaminated groundwater, and pumping the resulting solutions through the electron beam. These large-scale experiments, although necessary to demonstrate the commercial viability of the process, require a great deal of time and effort. This paper compares the results of large-scale electron beam irradiations to those obtained from bench-scale irradiations using gamma rays generated by a 60Co source. Dose constants from exponential contaminant removal models are found to depend on the source of radiation and initial contaminant concentration. Possible reasons for observed differences such as a dose rate effect are discussed. Models for estimating electron beam dose constants from bench-scale gamma experiments are presented. Data used to compare the removal of organic compounds using gamma irradiation and electron beam irradiation are taken from the literature and a series of experiments designed to examine the effects of pH, the presence of turbidity, and initial concentration on the removal of various organic compounds (benzene, toluene, phenol, PCE, TCE and chloroform) from simulated groundwater.

  12. A comparison of large-scale electron beam and bench-scale 60Co irradiations of simulated aqueous waste streams

    International Nuclear Information System (INIS)

    Kurucz, Charles N.; Waite, Thomas D.; Otano, Suzana E.; Cooper, William J.; Nickelsen, Michael G.

    2002-01-01

    The effectiveness of using high energy electron beam irradiation for the removal of toxic organic chemicals from water and wastewater has been demonstrated by commercial-scale experiments conducted at the Electron Beam Research Facility (EBRF) located in Miami, Florida and elsewhere. The EBRF treats various waste and water streams up to 450 l min -1 (120 gal min -1 ) with doses up to 8 kilogray (kGy). Many experiments have been conducted by injecting toxic organic compounds into various plant feed streams and measuring the concentrations of compound(s) before and after exposure to the electron beam at various doses. Extensive experimentation has also been performed by dissolving selected chemicals in 22,700 l (6000 gal) tank trucks of potable water to simulate contaminated groundwater, and pumping the resulting solutions through the electron beam. These large-scale experiments, although necessary to demonstrate the commercial viability of the process, require a great deal of time and effort. This paper compares the results of large-scale electron beam irradiations to those obtained from bench-scale irradiations using gamma rays generated by a 60 Co source. Dose constants from exponential contaminant removal models are found to depend on the source of radiation and initial contaminant concentration. Possible reasons for observed differences such as a dose rate effect are discussed. Models for estimating electron beam dose constants from bench-scale gamma experiments are presented. Data used to compare the removal of organic compounds using gamma irradiation and electron beam irradiation are taken from the literature and a series of experiments designed to examine the effects of pH, the presence of turbidity, and initial concentration on the removal of various organic compounds (benzene, toluene, phenol, PCE, TCE and chloroform) from simulated groundwater

  13. Estudo da radiosensibilidade ao 60CO do Vibrio cholerae O1 incorporado em ostras Radiosensibility of Vibrio cholerae o1 incorporated in oysters, to 60CO

    Directory of Open Access Journals (Sweden)

    Ivany R de Moraes

    2000-02-01

    Full Text Available OBJETIVO: Avaliar a eficiência da radiação ionizante por 60CO na eliminação de Vibrio cholerae O1, El Tor Ogawa, não-toxigênico, incorporados laboratorialmente em ostras vivas da espécie Crassostrea brasiliana. MÉTODO: Foram selecionadas amostras de ostras provenientes de Canan��ia (litoral sul de São Paulo, Brasil, as quais foram contaminadas com Vibrio cholerae e irradiadas com doses de 0,5 kGy e 1,0 kGy. RESULTADOS: Foram observadas diminuições significativas do número inicial do microrganismo indicado: de 3,4.10(7 para 10³ e 10², respectivamente. Os valores de D10 correspondentes foram de 0,173 a 0,235. CONCLUSÃO: Adotando-se o fator 6 como nível de segurança, conclui-se que a dose de irradiação de 1,41 kGy é necessária para eliminar números elevados de células viáveis de V. cholerae em ostras. Os experimentos foram realizados com os controles respectivos.OBJECTIVE: Evaluate the effect of ionizing irradiation by 60Co on Vibrio cholerae O1, El-Tor, Ogawa, non-toxigenic, incorporated in live oysters Crassostrea brasiliana. METHODS: Samples of oysters were selected from Cananéia town in the South coast of S. Paulo state, Brazil, contaminated with Vibrio cholerae and irradiated with 60Co at 0.5 and 1.0 kGy dosages. RESULTS: Showed significant reductions of the initial number of V. cholerae , ranging from 3.4 x10(7 to 10³ and 10², respectively. The D10 values related with the respective doses of irradiation were 0.173 and 0.235. CONCLUSION: Considering a 6 value as safety factor, it is concluded that 1.41 kGy irradiation dosage is necessary to eliminate a high number of V. cholerae viable cells in oysters. Controls were used in the experiment.

  14. Effect of 60Co-γ ray irradiation on green coffee beans, 1

    International Nuclear Information System (INIS)

    Tomoda, Goro; Matsuyama, Jun; Hiramoto, Keiko; Izu, Kumie

    1977-01-01

    Green coffee beans were irradiated with 60 Co-γ rays at doses of 0, 0.05, 0.5, 5.0 and 10.0 Mrad and the changes of general components in green and roast coffee beans were investigated together with those of the organoleptic properties of roast beans during storage according to the cup testing. In case of Brazil santos beans, irradiation of some 0.05 Mrad 60 Co-γ ray gave rather favourable mild flavour and no harmful influence on the quality of coffee, and moreover, would tend to extend the shelf life of roast beans. But influence of irradiation on the quality of coffee differed somewhat between two cultivars, Brazil santos and Colombia. (auth.)

  15. The teratogenic effects of low dose 60Co γ-rays on the early pregnant rats

    International Nuclear Information System (INIS)

    Lu Chunlin

    1991-01-01

    The pregnant Wistar rats were exposed to 0.5 Gy and 1.0 Gy 60 Co γ-rays at the 9th day after conception. The results: 60 Co γ-rays at dose of 1.0 Gy could induced many defects: excenphaly, hydrocephalus, gastroschisis, cleft palate and cleft lip, anophthalmia, microphthalmia, shorten tail and absent tail in surviving fetuses. The growth retardation was found from the parameters of fetal weight, height, head circle and development of skeleton. In the group of radiation dose 0.5 Gy, only hydrocephalus, absent tail and growth retardation of skeleton appeared. The results suggest that low-dose exposure in the early pregnant rats can induce fetal defects and growth retardation. The probable mechanism of teratogen and growth retardation was discussed. The cAMP levels of brain and liver of rat fetuses were reported

  16. Application of 60Co γ-ray irradiated polystyrene microplate for anti-HCV ELISA

    International Nuclear Information System (INIS)

    Feng Bo; Zeng Hongyan; Tang Yufang; Wang Lu

    2005-01-01

    In order to explore the effect of 60 Co γ-ray irradiation on minor polypeptides absorption of polystyrene microplate, an indirect ELISA detection of anti-HCV was established, 60 Co γ-ray irradiated polystyrene microplates and the controls (without irradiation or UV-irradiated) were applied to absorb recombinant HCV antigens respectively. Cooperated with Bovine antihuman IgG labelled HRP, their related indices of sensitivity, specificity, homogeneity and stability were determinate. The results indicated that, optimum dose of the γ-ray irradiation is 8 kGy, and compared with the controls, detection sensitivity and homogeneity of the polystyrene microplate irradiated to 8 kGy could be improved markedly. (authors)

  17. Pathomorphology of spleen lymphocyte apoptosis in large dose 60Co γ-irradiated mice

    International Nuclear Information System (INIS)

    Gao Linlu; Cui Yufang; Yang Hong; Xia Guowei; Peng Ruiyun; Gao Yabing; Wang Dewen

    2000-01-01

    Objective: The aim of the authors was to investigate the pathomorphology changes of spleen lymphocyte apoptosis after 60 Co γ-irradiation. Methods: The mice were irradiated with 6, 9, 12, 15 and 20 Gy of 60 Co γ-rays. At different times after irradiation, the mice were sacrificed and the pathological changes of spleen lymphocyte were observed by light and transmission electron microscopies. Results: Spleen lymphocyte decreased evidently and the peak of apoptosis in spleen lymphocyte was dependent on radiation dose and the time after irradiation. Conclusion: After γ-irradiation with large doses, pathological changes of spleen lymphocyte apoptosis in mice can be divided into obviously different stages. The main causes of death of spleen lymphocytes are different in different dose groups

  18. Effect of 60Co γ-rays on polyphenyl methacrylate obtained by γ-ray irradiation

    International Nuclear Information System (INIS)

    Raghunath, S.; Rao, M.H.; Rao, K.N.

    1983-01-01

    Polyphenyl methacrylate of different molecular weights Msub(n) 13000, 34500 and crosslinked polymer prepared by 60 Co γ-irradiation has been irradiated with 60 Co γ-rays at 300 K. G(scission) of the 13000 molecular weight polymer was 15. The crosslinked polymer undergoes both degradation and crosslinking. G(x) value determined for that polymer is approx. 9. The gaseous product consists largely of CO with some CO 2 and H 2 . Hydrocarbon gases were not detected. The yield of H 2 is only to the extent of 2 to 5% of the total gas and it is assumed that majority of scission takes place at the ester linkage. On the basis of the estimation of phenol and benzene, a mechanism for degradation and crosslinking is proposed. (author)

  19. Doses in sensitive organs during prostate treatment with a 60Co unit

    International Nuclear Information System (INIS)

    Vega-Carrillo, H.R.; Navarro Becerra, J.A.; Pérez Arrieta, M.L.; Pérez-Landeros, L.H.

    2014-01-01

    Using thermoluminiscent dosimeters the absorbed dose in the bladder, rectum and thyroid have been evaluated when 200 cGy was applied to the prostate. The treatment was applied with a 60 Co unit. A water phantom was built and thermoluminiscent dosimeters were located in the position where the prostate, bladder, rectum and thyroid are located. The therapeutic beam was applied in 4 irradiations at 0, 90, 180 and 270° with the prostate at the isocenter. The TLDs readouts were used to evaluate the absorbed dose in each organ. The absorbed doses were used to estimate the effective doses and the probability of developing secondary malignacies in thyroid, rectum and bladder. - Highlights: • The absorbed doses in the bladder, rectum and thyroid were measured. • Measurements were done during prostate treatment with a 60 Co unit. • TLD100s in a water phantom were used. • The effective doses were also estimated

  20. Long-term clearance of accidentally inhaled 60Co aerosols in humans

    International Nuclear Information System (INIS)

    Beleznay, E.; Osvay, M.

    1993-03-01

    Long-term body retention was measured on six workers inhaling 60 Co aerosols incidentally during manipulation with a high activity 60 Co source. An improved whole body counter technique and calibration provided good conditions to follow the body clearance over 4 years. A two-detector profile scanning arrangement was used to measure the activity distribution 'in vivo' over the lung region. The observed whole body retention followed a two-exponential time function between 10-1500 days. The consistency of the measured retention pattern with ICRP inhalation model was investigated assuming different aerosol size distributions. A possible extension of ICRP lung model is proposed to take into account the actual pulmonary lung clearance determined individually. (R.P.) 29 refs.; 15 figs.; 6 tabs

  1. Activation cross section of 63Cu(n,α)60Co reaction

    International Nuclear Information System (INIS)

    Lu Hanlin; Zhao Wenrong; Yu Weixiang; Yuan Xialin

    1990-01-01

    The mechanical properties of copper during the irradiation with intensive neutron fluence rate are significant for the safe operation of D-T fusion power reactors. The cross sections measured by activation method show a large discrepancy from 36 to 54 mb in 14 MeV region. The cross sections of 69 Cu(n, α) 60 Co reaction were measured by activation method. Two irradiations were carried out at the Cockcroft-wallon and Van de Graaff accelerators of CIAE using T(d, n) 4 He reaction to produce neutrons. The activities of 24 Na and 60 Co γ-rays for monitor and sample foils were determined absolutely by a calibrated Ge(Li) detector system with an accuracy better than 1%. The present results are compared with the others

  2. Effect of 60Co γ-ray irradiation on human platelets

    International Nuclear Information System (INIS)

    Wu Guoxin; Zhao Yiming; Ruan Changgeng

    1992-01-01

    Human platelet-rich plasma was irradiated with various doses (0, 1.25, 2.5, 5, 10, 20, 40 Gy) of 60 Co γ-rays in order to observe the changes of metabolites and the release of internal substance of platelets. At the dose of 5 Gy, alpha-granule membrane protein (GMP-140) molecules expressed on the surface of platelets increased significantly, while glycoproteins (GP) Ib and IIIa did not change apparently; at the dose as low as of 2.5 Gy, thromboxane B 2 production in plasma was remarkably increased; and, at the dose of over 5 Gy, the concentration of von Willebrand factor increased with increasing doses as in the case of GMP-140 molecules. These results indicate that platelets can be activated in vitro when the dose of 60 Co γ-rays exceeds 5 Gy

  3. Analysis of the microbial growth in 60Co γ-irradiated foods by calorimetry

    International Nuclear Information System (INIS)

    Furuta, Masakazu; Hayashi, Toshio; Hamasaki, Koji; Wirkner, Sandra; Constantinoiu, Elena; Takahashi, Katsutada

    2002-01-01

    Using a heat conduction calorimeter equipped with 24 sample units the heat evolutions from growing 60 Co γ-irradiated bioburden of black pepper seeds and frozen beef were detected in the form of growth thermograms. 60 Co γ-irradiation affected the growth pattern in which a dose-dependent reduction of the growth rate constant was observed together with the retardation in growth, indicating a combination of bactericidal and bacteriostatic effects. We successfully determined the minimal inactivation doses for the two food samples using the relationship between the irradiation dose and the retardation in growth t α , or the growth rate constant μ obtained from the growth thermograms. These results strongly suggested the possibility of calorimetry as measure of predictive microbiology in food irradiation. (author)

  4. Hassall's corpuscles in the guinea-pig thymus after 60Co 2000R local irradiation

    International Nuclear Information System (INIS)

    Kinoshita, Hideo; Sugiyama, Shizuyuki; Shibata, Moritoshi; Ishibashi, Hirofumi; Uehara, Masaomi

    1974-01-01

    The cervical regions of male guinea pigs weighing about 300g were irradiated locally with 60 Co 2000 R and the animals were sacrificed on the 3rd, 5th, 7th and 9th days after the irradiation. The thymus was weighed and fixed in 10% formal saline. Tissues were blocked in paraffin and each block was cut into serial sections having a thickness of approximately 6 microns. These sections were stained by the haematoxylin-eosin (HE) and the aldehyde-fuchsin (AF) techniques. The size of Hassall's corpuscles was measured in the sections stained with haematoxylin-eosin and it was found that the weight of the thymus in 60 Co 2000 R irradiation decreased remarkably and that the tendency of Hassall's corpuscles to enlarge was more remarkable in the 2000 R group than in the 800 R group. (author)

  5. Hassall's corpuscles in the guinea-pig thymus after /sup 60/Co 2000R local irradiation

    Energy Technology Data Exchange (ETDEWEB)

    Kinoshita, H; Sugiyama, S; Shibata, M; Ishibashi, H; Uehara, M [Nara Medical Univ., Kashihara (Japan)

    1974-12-01

    The cervical regions of male guinea pigs weighing about 300g were irradiated locally with /sup 60/Co 2000 R and the animals were sacrificed on the 3rd, 5th, 7th and 9th days after the irradiation. The thymus was weighed and fixed in 10% formal saline. Tissues were blocked in paraffin and each block was cut into serial sections having a thickness of approximately 6 microns. These sections were stained by the haematoxylin-eosin (HE) and the aldehyde-fuchsin (AF) techniques. The size of Hassall's corpuscles was measured in the stained sections and it was found that the weight of the thymus in /sup 60/Co 2000 R irradiation decreased remarkably and that the tendency of Hassall's corpuscles to enlarge was greater in the 2000 R group than in the 800 R group.

  6. Effect of 60Co γ radiation on hexobarbital-induced sleeping times in rats

    International Nuclear Information System (INIS)

    Brady, M.E.; Hayton, W.L.

    1977-01-01

    The duration of the loss of righting reflex (sleeping time) was determined after intraperitoneal administration of 100 mg(/kg hexobarbital to adult male Sprague--Dawley rats that were exposed to 60 Co γ radiation or sham irradiated. No difference between irradiated and control animals was detected at 1, 3, 5, and 7 days after 850 R. Also, no difference between irradiated and control animals was detected at 5 days after 1000 or 1400 R

  7. Image acquisition, transmission and assignment in 60Co container inspection system

    International Nuclear Information System (INIS)

    Wu Zhifang; Zhou Liye; Liu Ximing; Wang Liqiang

    1999-01-01

    The author describes the data acquisition mode and image reconstruction method in 60 Co container inspection system, analyzes the relationship between line pick period and geometry distortion, makes clear the demand to data transmitting rate. It discusses several data communication methods, draws up a plan for network, realizes automatic direction and reasonable assignment of data in the system, cooperation of multi-computer and parallel processing, thus greatly improves the systems inspection efficiency

  8. Introduction of beam flatness filter for 60Co teletherapy beam and its efficacy in clinical radiotherapy

    International Nuclear Information System (INIS)

    Sathiyan, S.; Ravichandran, R.; Ravikumar, M.

    2003-01-01

    In the western countries cobalt-60 machines have become obsolete, whereas in India we have about 250 machines operational for clinical radiotherapy. The basic differences of 6 MV x-ray beam and cobalt-60 beam are: a) build-up d max point, b) flatness of beam at depths, and c) sharpness of the beam edge. We looked at the homogeneity of delivered dose in the target volume in a 3 field SAD technique for 60 Co (80 cm, 100 cm) and 6 MV treatments. More dose variations are seen in 60 Co treated volume. The excess curvature of isodose curves of 60 Co at depths may be one of the reasons for this inhomogeneity in dose to target volume. Therefore, there is need for achieving perfect flatness in the isodose curves at desired depths. A flattening filter was fabricated using dental wax impression material to account for depths of curvature of 50% 60 Co isodose curve. The filter was fabricated for the Theratron 780C machine for necessary flatness. The beam flatness with filter was measured with a) ionization and b) TL dosimetry methods. The flattened beam profile was compared with 6 MV x-ray beam (Clinac-1800, M/s Varian, USA). Our measurements show uniform flatness of cobalt-60 isodose curve at desired depth and useful radiation field width comparable to 6 MV x-ray photon profile at full width at half maximum (FWHM). If this concept is extendable to short field widths, it appears that there is scope for use of such filter in the treatments of oesophagus, larynx, and pituitary tumours to achieve dose homogeneity. Using this flatness filter and penumbra trimmer, we may achieve better quality cobalt-60 beam for radiotherapy. (author)

  9. Preparation of high water-swelling agricultural starch hydrogels by 60Co γ-radiation grafting

    International Nuclear Information System (INIS)

    Wang Qingjun; Quan Yiwu; Chen Qingmin

    2003-01-01

    The starch grafted acrylic acid was used to synthesize water-swelling hydrogels by 60 Co γ-radiation grafting technique. With radiation dose of about 7 kGy, the crosslinking reagent amount of 0.001%-0.1%, the pH value 5-8 and the starch amount of 10%-30%, we can produce 600 times water-swelling hydrogels which are of high performance, low cost and suitable for agriculture

  10. Study on the possibility of using a 60 Co therapeutical unity in Intensity Modulated Radiotherapy (IMRT)

    International Nuclear Information System (INIS)

    Dantas, Samuel Cesar

    2009-06-01

    With the increasing advances in complex treatment techniques, there is a tendency to obtain more sophisticated equipment to deliver the dose. The use of 3D conformal radiotherapy is now routine in many radiotherapy facilities as well as the utilization of intensity modulated radiotherapy (IMRT). Both are usually implemented using linear accelerators equipped with multi leaves collimators, which create the conformity and the fluence distributions required. However, the complexity of increasingly sophisticated equipment, such as linear accelerators, requires a frequent quality control of their operation, as well as a detailed and constant maintenance. Even carrying out these procedures, the accelerators may present technical problems interrupting for a long time a treatment using the IMRT technique. Despite the clear practical and technological advantages that linear accelerators have on 60 Co irradiators, these devices occupy an important place in radiotherapy, mainly due to the low cost of equipment installation and maintenance when compared to those required by accelerators. Many radiotherapy facilities that work with IMRT have tele therapeutic isocentric 60 Co units. In principle, such equipment would be able to be used for treatment with IMRT using compensating blocks to modulate the beam. This study investigates this possibility and shows that it is feasible. The comparison of treatment plans of a head-and-neck cancer and other of a cancer of the central nervous system, based on a 60 Co irradiator and a Linac 2300 C/D, presented advantages for the 60 Co irradiator. Furthermore; the delivery of dose obtained with the two systems showed themselves equivalent when compared to their respective plans. (author)

  11. Studies on bioaccumulation of 152Eu, 137Cs, 60Co by different algal genera

    International Nuclear Information System (INIS)

    Saha, A.K.; Chakraborty, N.; Pal, R.; Nayak, Dalia; Lahiri, Susanta

    2006-01-01

    In the present paper, the bioaccumulation of a mixture of potentially hazardous, long lived radionuclides 152 Eu, 137 Cs and 60 Co by five algal genera of three different family, viz.: Lyngbya and Phormidium from cyanophyceae; Phizoclonium and Chaetomorpha from chlorophyceae and Catenella from rhodophyceae have been studied. Because of the long half life and high solubility, these radionuclides are considered the most hazardous in the nuclear spent fuel

  12. Measurement of exposure dose rate by TLD in 60Co garden

    International Nuclear Information System (INIS)

    Fan Chengfang; Li Pinfang; Wu Maoliang; Zhao Quan

    1987-01-01

    The use of TLD method to measure exposure dose rate in 60 Co garden is described. As compared with chemical dosimetry, the relative deviation of measurement would not exceed 10%. The good repeatability has been proved by comparing the measurements of different times and distances with correction of decay law and 1/1 2 law. Besides, the distribution of exposure dose rate was influenced by scattering of the living plants

  13. 60Co accumulation from sediment and planktonic algae by midge larvae (Chironomus luridus)

    International Nuclear Information System (INIS)

    Baudin, J.P.; Nucho, R.

    1992-01-01

    This paper reports the results of several experiments carried out to evaluate uptake and retention by a limicolous midge larva of 60 Co retained in sediment, either adsorbed on mineral particles or bound to planktonic algae. In order to determine their relative contributions in radionuclide accumulation, the different vectors (water, algae and sediment) were first labelled individually and then simultaneously. 60 Co accumulation from water and from algae results in a maximum concentration factor of 30 and in a mean trophic transfer factor of 4·5 × 10 −3 . The level of contamination of midge larvae from sediment is markedly influenced by the presence of endogenous organic matter. Thus the radionuclide transfer factor is about twice as high for larvae placed in labelled raw sediment than for larvae placed in labelled incinerated sediment, in the presence as in the absence of contaminated planktonic algae. Irrespective of the contamination conditions, 60 Co depuration from midge larvae is a very rapid phenomenon that corresponds, in all cases, to a radionuclide half-life of only a few days

  14. Biological effects and ISSR analysis of 60Co γ-irradiation on edible mushroom mycelia

    International Nuclear Information System (INIS)

    Cai Weiming; Feng Weilin; Jin Qunli; Fan Lijun; Wu Yongzhi

    2009-01-01

    Mycelia of Agaricus bitorquis, Flammulina velutipes and Pleurotus eryngii were irradiated by 60 Co γ-rays at the dose of 0, 200, 500 and 800 Gy. The results showed that irradiation inhibited their mycelia growth positively. Of the three edible mushroom, Agaricus bitorquis was the most sensitive one to irradiation, its mycelia died at the dose of 800 Gy. Germination rate, growth rate and vigor of all mycelia irradiated with un-lethal doses increased along with the times of transfer of culture. Electrical conductivity (EC) analysis showed that irradiation increased EC of leaching solution of mycelia positively. The EC of leaching solution of mycelia of Flammulina velutipes and Pleurotus eryngii irradiated with 500 Gy, Agaricus bitorquis irradiated with 200 Gy, were significantly higher than CK. ISSR analysis revealed that 60 Co γ irradiation effect on DNA variation of Agaricus bitorquis, Flammulina velutipes and pleurotus eryngii mycelia in varying degrees. Number of polymorphic bands changed after irradiation. Flawing bands and increasing bands were observed in amplified bands of 14 selected ISSR primers. The ISSR variation rates of Agaricus bitorquis for 200 Gy and 599 Gy treatments were 33.3% and 50.5%; the ISSR variation rates of Flammulina velutipes for 200 Gy, 500 Gy and 800 Gy treatments were 2.3%, 2.3% and 3.1%, that of Pleurotus eryngii were 8.6%, 13.4% and 20.0%. It is possible to use 60 Co γ irradiation as a mutagen to screen new mushroom varieties. (authors)

  15. Analysis of ZPPR experiments supporting production of 60Co in FFTF

    International Nuclear Information System (INIS)

    Wootan, D.W.; Carter, L.L.; Rawlins, J.A.; Schaefer, R.W.; Maddison, D.W.

    1987-01-01

    An effort to expand the irradiation mission of the Fast Flux Test Facility (FFTF) beyond testing fuels and materials for the liquid-metal reactor (LMR) program included a study of the feasibility of producing commercial quantities of 60 Co. The 60 Co would be produced by neutron capture in assemblies containing an array of natural cobalt pins and hydrogen-bearing moderator pins located at the periphery of the FFTF core. Adding hydrogenous material to the assemblies enhances 60 Co production by slowing neutrons into energy ranges where the 59 Co capture cross section is higher. Some of the moderated neutrons leak from the moderated region to adjacent fuel regions, increasing local fission rates. In order to validate calculated fission rates and establish calculational biases and uncertainties, experiments were conducted in the zero-power plutonium reactor (ZPPR) to measure fission rates in fuel regions adjacent to a mock-up of a cobalt production assembly. The ZPPR experiment and analyses provided the technical basis for irradiating hydride-moderated assemblies in FFTF and a cobalt production cycle of FFTF with generally good agreement between measured and predicted reactivity worth and outlet temperatures in adjacent fuel assemblies. This experiment provides a benchmark configuration of a hydrogenous assembly in a fast reactor, with emphasis on the effect of the hydrogen on adjacent fuel. It is anticipated there will be other applications of hydride moderation in the development of future isotope production missions in FFTF

  16. The mutant strain of ZHJ6 degrading organophosphorous pesticide by 60Co-γ irradiation

    International Nuclear Information System (INIS)

    Zhao Renbang; Chi Jian; He Yi

    2013-01-01

    The strain of Penicillium oxalicum ZHJ6 that can degrade methamidophos was employed to obtain the mutant stain which has higher degradation rate than original strain by 60 Co-γ irradiation. Results showed that the Penicillium oxalicum ZHJ6 was sensitive to 60 Co-γ irradiation, and was easy to be killed by 60 Co-γ irradiation. Under the absorbed dose of 2.1 kGy, the survival rate of the strain was 0.04%. Two strains of A17 and A18 were obtained from the irradiated strains after first- and second- screening and the degradation rate of methamidophos of A17 and A18 strains were 10% higher than that of A0 strain (original stain). Moreover, the abilities to degrade folimat, phoxim and glyphosate were improved. Through 5 generations, the variation coefficient in degradation rate of methamidophos in the 6th day was 1.2%, showing that the new strains had hereditary stability. (authors)

  17. Leaching of 60Co, 137Cs and 226Ra in lysimeter experiments

    International Nuclear Information System (INIS)

    Gerzabek, M.H.; Mueck, K.; Steger, F.; Algader, S.M.

    1996-10-01

    The present report describes the first results obtained from lysimeter experiments started in 1990. The lysimeter plant consists of twelve soil monoliths from four different sites (three replicates each). Since 1990 the following agricultural crops were grown: endive, corn, winter wheat, mustard, sugar beet and potato. Gravitational water ranged from 3.9 % to 18.3 % of precipitation plus irrigation water, calculated as half years average values excluding the first six months of operation. The two Cambisols on sediments exhibited a mean percentage of approx. 10 %. The Dystric Cambisol on silicate rock and the Gleysol showed average values of 20 %. In 1990 the top layers (20 cm) were contaminated with three radionuclides. The leaching of the contaminants 60 Co, 137 Cs and 226 Ra differed distinctly between the elements. The lowest leaching rates were observed for 137 Cs, followed by 60 Co. The 226 Ra-concentrations in the gravitational water were clearly highest of all. However, the comparison of the 226 Ra/ 22 2 8 Ra ratios in deep soil layers and in gravitational water showed that the 226 Ra measured in the leachate originates from the natural contents in the subsoil and not from the artificial 226 Ra contamination of the topsoil. The mobility of the artificial radionuclides decreased therefore in the following order: 60 Co > ( 226 Ra) > 137 Cs. (author)

  18. 60Co accumulation from sediment and planktonic algae by midge larvae (Chironomus luridus)

    International Nuclear Information System (INIS)

    Baudin, J.P.; Nucho, R.

    1992-01-01

    This paper reports the results of several experiments carried out to evaluate uptake and retention by a limicolous midge larva of 60 Co retained in sediment, either adsorbed on mineral particles or bound to planktonic algae. In order to determine their relative contributions in radionuclide accumulation, the different vectors (water, algae and sediment) were first labelled individually and then simultaneously. 60 Co accumulation from water and from algae results in a maximum concentration factor of 30 and in a mean trophic transfer factor of 4.5 x 10 -3 . The level of contamination of midge larvae from sediment is markedly influenced by the presence of endogenous organic matter. Thus the radionuclide transfer factor is about twice as high for larvae placed in labelled raw sediment than for larvae placed in labelled incinerated sediment, in the presence as in the absence of contaminated planktonic algae. Irrespective of the contamination conditions, 60 Co depuration from midge larvae is a very rapid phenomenon that corresponds, in all cases, to a radionuclide half-life of only a few days. (author)

  19. Effects of 60Co γ-radiation on brain hippocampal tissue of adult mice

    International Nuclear Information System (INIS)

    Liu Yongbao; Rao Yongqing; Xu Luxi

    2000-01-01

    Objective: To study neuro-pathological changes of hippocampus tissue in adult mice following a series of irradiation with 60 Co γ-rays. Methods: Male mice of Kunming strain in experimental group (n = 8) were exposed total-bodily to 60 Co γ-rays at 2.0 Gy once every two days. A histopathological imaging analysis of the mouse brain tissue was carried out after paraffin embedding and a series of sections were made and stained with Nissl and Weil staining methods. Results: In the irradiation group (the cumulative dose = 26 Gy) loss of pyramidal cells in hippocampus was significant when compared with the control group. Neuro-pathological changes were characterised by reduced neuron size, nuclear pyknosis and karyolysis. The neurofibrillar density of the pyramidal layer in the irradiation group was much lower than that of the control group (P CA2>CA3>CA4 in the hippocampus. Conclusion: The neuronal damage in hippocampus after 60 Co irradiation could form a pathological basis in reduction of memorial and learning ability

  20. Decontamination of salmonella from the coastal fish meals by 60Co γ ray irradiation

    International Nuclear Information System (INIS)

    Saheki, Kazuaki; Konno, Kenjiro; Sato, Takae; Kawabata, Toshiharu.

    1988-01-01

    The decontamination fo salmonellae from fish meal samples by irradiation which 60 Co γ ray was examined. Sixteen strains of Salmonella were used. A composite fish meal sample was prepared by mixing with different coastal meals, and after radiation sterilization at a dose of 20 kGy 60 Co γ ray, which was employed as the salmonella-free meal sample. D 10 values of test strains determined in buffered saline were found to range from 0.08 to 0.36 kGy, and inactivation factors at a dose of 1 kGy ranged from 10 2.8 to 10 13 . D 10 values of test strains determined in the salmonella-free meal sample ranged from 0.59 to 1.64 kGy, and the inactivation factors at a dose of 10 kGy were found to range from 10 6.1 to 10 17 . Interestingly the D 10 values of salmonellae determined in fish meal samples were 10 times as much compared with those determined in buffered saline. From the commercial aspects of coastal fish meal production, destruction of salmonellae in fish meals by 60 Co γ ray irradiation was found to be much more practical than other methods such as dry heating and ethylene oxide fumigation. (author)

  1. In vitro response of the human breast cancer cell line MDAMB-231 and human peripheral blood mononuclear cells exposed to 60Co at single fraction

    International Nuclear Information System (INIS)

    Andrade, Lidia Maria; Campos, Tarcisio Passos Ribeiro de; Leite, M.F.; Goes, A.M.

    2005-01-01

    Radiotherapy using gamma rays is a common modality of breast cancer treatment. The aim of this research is to investigate the biological response of the human breast cancer cell line MDAMB-231 and human peripheral blood mononuclear cells (PBMC) exposed in vitro to 60 Co irradiation at a single fraction of 10 Gy, 25 Gy and 50 Gy doses at 136,4 cGy.min -1 rate. Cells were irradiated at room temperature by the Theratron 80 radiotherapy system. Biological response was evaluated through cellular viability using MTT assay and nucleus damages visualized by Propidium Iodide assay and electrophoresis agarose gel after gamma irradiation. Nucleus damages induced by 60 Co irradiation were compared to damage caused by cell exposure to 10% methanol. The 50 Gy dose of irradiation did not stimulate nucleus damages at the same level as that affected by 10% methanol induction in the MDAMB-231. Further studies are necessary to understand these mechanisms in the MDAMB-231 human breast carcinoma cell line.(author)

  2. In vitro response of the human breast cancer cell line MDAMB-231 and human peripheral blood mononuclear cells exposed to {sup 60}Co at single fraction

    Energy Technology Data Exchange (ETDEWEB)

    Andrade, Lidia Maria; Campos, Tarcisio Passos Ribeiro de [Universidade Federal de Minas Gerais, Belo Horizonte, MG (Brazil). Dept. de Engenharia Nuclear]. E-mail: lidia.andrade@unifenas.br; Leite, M.F. [Universidade Federal de Minas Gerais, Belo Horizonte, MG (Brazil). Dept. de Fisiologia e Biofisica; Goes, A.M. [Universidade Federal de Minas Gerais, Belo Horizonte, MG (Brazil). Dept. de Bioquimica e Imunologia

    2005-10-15

    Radiotherapy using gamma rays is a common modality of breast cancer treatment. The aim of this research is to investigate the biological response of the human breast cancer cell line MDAMB-231 and human peripheral blood mononuclear cells (PBMC) exposed in vitro to {sup 60} Co irradiation at a single fraction of 10 Gy, 25 Gy and 50 Gy doses at 136,4 cGy.min{sup -1} rate. Cells were irradiated at room temperature by the Theratron 80 radiotherapy system. Biological response was evaluated through cellular viability using MTT assay and nucleus damages visualized by Propidium Iodide assay and electrophoresis agarose gel after gamma irradiation. Nucleus damages induced by {sup 60} Co irradiation were compared to damage caused by cell exposure to 10% methanol. The 50 Gy dose of irradiation did not stimulate nucleus damages at the same level as that affected by 10% methanol induction in the MDAMB-231. Further studies are necessary to understand these mechanisms in the MDAMB-231 human breast carcinoma cell line.(author)

  3. Relative biological efficiency of intermediate energy neutrons and 60Co rays for induction of chromosomal aberrations in Chinese hamster fibroblasts

    International Nuclear Information System (INIS)

    Sturelid, S.; Bergman, R.

    1976-01-01

    Intermediate energy neutrons are unique in that a considerable fraction of critical interactions and of dose absorbed is not associated with ionization but with atomic collision. It is still unknown to what extent the qualitative difference in primary damage after atomic collision compared to that of ionization and excitation becomes expressed at biological levels. Chromosomal aberrations were studied in Chinese hamster fibroblasts exposed for 5-8 hours at 22 degree C to intermediate energy neutrons, mean energy 8.5 keV, or to 60 Co-gamma rays. RBE at the 10 per cent aberration frequency level in S-phase were 2.2+-0.6 for total aberrations, 2.1+-0.6 for chromatid breaks and 1.8+-0.5 for exchanges. For each chromatid aberration observed after recovery, about 200 bondbreaking atomic collisions besides 3000 primary iniozations should have occured in DNA. However, the extent to which the aberration response is due to atomic collisions is not clear. (author)

  4. Effect of cobalt on the growth of Trichoderma viride and determination of its distribution using 60Co

    International Nuclear Information System (INIS)

    Frank, V.; Lesny, J.; Babej, L.

    1993-01-01

    The growth and morphology of filamentous fungus Trichoderma viride grown in the presence of various Co concentrations were studied. Using 60 Co, the cobalt content in mycelium and conidia was determined. No influence of 60 Co-radiation on growth and morphology was observed. (author) 5 refs.; 4 figs

  5. Obtained and evaluation of antisera raised against irradiated crotalic whole venom or crotoxin in 60 Co source

    International Nuclear Information System (INIS)

    Paula, Regina A. de.

    1995-01-01

    Snake bite is a great Public Health problem in our country. The accidents with snakes from Crotalus genus are the most severe. About 1% of the victims die without seratherapy. The antivenons are obtained from hyper immune horse plasma. During the production these animals present signs of envenoming that result in a decrease of organic resistance besides the horses maintenance is very expensive and the producers are fewer, so the sera production is restrict. Many techniques which could reduce the venoms toxicity and increase the sera production using chemical and physical agents have been studied. The gamma rays are excellent tool to detoxify venoms and toxins. It is able to modify protein structures that decrease lethally, toxic and enzymatic activities without modifying the immunogenicity. So, it is important evaluate the sera production in rabbits using gamma rays detoxified venom and crotoxin as immunogen and their power as reagents in immuno assays. In order to obtain the antisera, Crotalus durissus terrificus whole venom or isolated crotoxin was irradiated with 2.000 Gy in 60 Co source, in a 150 mM NaCl solution, and inoculated in rabbits. The sera production were screened by immunoprecipitation, immuno enzymatic (ELISA) and immunoradiometric (IRMA) assays. The specificity was studied by immuno-electrophoresis, ELISA and western blot techniques. The neutralizing power was evaluated by neutralization of phospholipase A 2 activity of toxin in vitro. The antisera were used as reagents in antigen capture assays ELISA and IRMA immuno assays to detect circulant antigens in sera of mice experimentally inoculated with crotalic venom or crotoxin. The results showed that both detoxified venom or crotoxin were good immunogens, and they were able to induce antibodies that could recognize non-irradiated venom or isolated crotoxin. The data suggest that those antibodies present more specificity and higher in vitro neutralizing power, when compared with commercial

  6. The effect of 60Co γ-rays on the contents of paramylon and trehalose in Euglena gracilis

    International Nuclear Information System (INIS)

    Furuta, M.; Maruyama, A.; Nakazawa, M.; Ueda, M.; Miyatake, K.; Kato, A.; Enomoto, T.

    2003-01-01

    Full text: It is reported that the non-reducing disaccharide trehalose is accumulated in various organisms and protects biomolecules against various environmental stresses including heat shock, osmotic shock, and oxidative stresses. Euglena (Euglena gracilis) is photosynthetic unicellular organisms which is classified both as animal and as plant because of its motility. In our previous study, it was shown that salt stress causes degradation of paramylon, the reserve carbohydrate of Euglena, and trehalose, the degraded product of paramylon, accumulated simultaneously to maintain the osmotic pressure of Euglena cells. Euglena is known to be radiation-resistant more than Escherichia coli and other bacteria. Therefore, we have investigated whether or not the same phenomenon could be observed in Euglena after 60 Co-gamma irradiation. Exposure of the native Z strain and chloroplast-deficient SM-ZK strain of Euglena to a dose of 500 kGy induced significant paramylon degradation and trehalose accumulation during the incubation in the Koren-Hunter culture medium containing glucose as carbon source after the irradiation. Forty percent of the paramylon was degraded within 10-hr incubation after the irradiation but accumulated trehalose content was about one sixth of that by salt stress. Trehlose accumulation disappeared when the irradiated cells were incubated in phosphate buffer but it recovered when glucose is added to the buffer to the same concentration as K-H media. These results suggest that trehalose accumulation in irradiated Euglena cells plays a role in protecting cellular constituents from oxidative damage caused by gamma-irradiation independently of the response to salt stress

  7. Optimization of point sources on thin films using a statistical design approach. Application to {sup 60}Co sources; Optimisation des sources ponctuelles sur film mince par une approche experimentale de type plan d'experiences. Application aux sources de {sup 60}Co

    Energy Technology Data Exchange (ETDEWEB)

    Sanoit, J.; Leprince, B. [CEA Saclay, Dept. d' Instrumentation et de Metrologie des Rayonnements Ionisants, 91 - Gif sur Yvette (France); Vailhen, D. [CEA Saclay, Dir. de la Surete Nucleaire et de la Qualite, 91 - Gif sur Yvette (France)

    2001-07-01

    Radioactive point sources prepared by depositing weighed drops on thin membranes are commonly used in ionizing radiation metrology for 47{pi}{beta}-{gamma} coincidence counting or for low energy electrons spectrometry. The present study deals with the preparation of good quality sources having high detection yields. Some influential parameters on the source quality are studied using {sup 60}Co as radioactive tracer. An approach based on experimental statistical designs has been chosen. This approach allows us to obtain reliable results with a number of experiences lower than in the case of a systematic approach. Homogeneous {sup 60}Co sources with high metrological qualifies (R{sub {beta}}> 96 %) can be prepared using a direct deposit of {approx} 5 mg drops of {sup 60}Co solution ([Co] = 12 {mu}g/g, [HCl] = 0,5 mol/I) and dried at 60 deg C. The support of the radioactive spot is constituted with a gold coated (130 {mu}g/cm{sup 2} of Au) Vyns films (thickness 45 {mu}g/cm{sup 2}) on which a pad (diameter = 7 - 8 mm) of polystyrene microspheres (Estapor K-007) is prepared by an electro-spraying technique under 7,5 kV during 10 minutes. The alcoholic solution used for the electro-spraying step has the following chemical composition [Estapor K-007] 2.5 10{sup -2} wt % and [Tween 20] = 2.7 10{sup -2} wt %. (authors)

  8. Hassall's corpuscles in the guinea-pig thymus after a second 60Co local irradiation

    International Nuclear Information System (INIS)

    Kinoshita, Hideo

    1974-01-01

    The cervical regions of male guinea-pigs, weighing about 300 g, were irradiated locally with 60 Co 800 R, and the same dose of irradiation was repeated a second time in each guinea pig at a different interval. Thereafter, an irradiated animal was sacrificed every other day for 2 weeks. Thymuses were weighed and fixed in 10 percent formal saline. Tissues were blocked in paraffin and each block was cut into serial sections having a thickness of approximately 6 microns. These sections were stained with the haematoxylin-eosin (HE) and the aldehyde-fuchsin (AF) techniques. The size of Hassall's corpuscles was measured in the sections of HE and the AF (+) ratio of Hassall's corpuscles was calculated in sections of AF. The ratio (%) of the number of AF (+) corpuscles to the total number of corpuscles seen in the entire microscopical field was called ''AF (+) ratio''. The weight of the twice irradiated thymuses decreased regardless of the interval from the first irradiation. The tendency of Hassall's corpuscles to enlarge during the period of enlargement of the corpuscles after first irradiation was increased by the second irradiation. The enlarged pattern of Hassall's corpuscles after a single irradiation of 60 Co 800 R re-appeared when the animals were given a second irradiation on the 35th day. Concerning the AF (+) ratio of Hassall's corpuscles, the increasing pattern of AF (+) ratio such as after single irradiation of 60 Co 800 R re-appeared when the animals were given a second irradiation on the 35th day, but no effect was observed during the process of enlargement and disintegration of Hassall's corpuscles after the second irradiation. The enlargement of Hassall's corpuscles is attributed not only to the process of lympholysis, but also to various other factors such as the number and size of the corpuscles and the stage in their life cycle. (JPN)

  9. Assessment of the mobility and bioavailability of 60 Co and 137 Cs in contaminated soils

    International Nuclear Information System (INIS)

    Wasserman, Maria Angelica; Bartoly, Flavia; Poquet, Isabel; Perez, Daniel V.

    2001-01-01

    Results of a classical sequential chemical extraction procedure for 137 Cs in an acid Oxisol showed that after 3 years of contamination radiocesium remains potentially available for transfer processes: 40% bio-available, 20% mobile under oxidizing conditions and 40% bound to Fe and Mn oxides (available under reducing conditions). At this time, the transfer factor obtained in this soil was higher than values obtained in basic Oxisol and was higher than values obtained in soils from temperate climate areas. Seven years after the contamination, the 137 Cs distribution in this acid Oxisol have been changed as consequence of changes in soil properties: 8% bioavailable, 16% mobile under oxidizing conditions, 43% bound to Fe and Mn oxides and 33% strongly bound to soil compounds. Changes in the 137 Cs distribution in this soil were followed by reductions in soil to plant transfer factor. Between 1996 and 2000, the 137 Cs distribution, 137 Cs soil to plant transfer factor and soil properties in the basic Oxisol remained almost the same. The 60 Co distribution showed that Mn oxides is the main sink for this element and four years after contamination no 60 Co was detected as bioavailable or detectable in plants. In this study the use of an alternative sequential chemical extraction protocol to evaluate 60 Co and 137 Cs mobility under a large range of physico-chemical soil properties has shown to be very consistent with soil to plant transfer factors data for maize. The knowledge of bio-geochemical behavior of radionuclides in soil system can be used for the risk assessment in the case of nuclear accident or contamination scenarios. (author)

  10. Comparison of treatment in soybean grains between 60Co and e-beans applications

    International Nuclear Information System (INIS)

    Fanaro, Gustavo B.; Araujo, Michel M.; Thomaz, Fernanda S.; Duarte, Renato C.; Villavicencio, Anna Lucia C.H.

    2007-01-01

    Soybean is the most important oleaginous cultivated in Brazil, who is the second largest exported in the world, and generates high incomes, direct and indirectly, its oils could be used since to cook even to machine's fuel and the nutrients become basic for the feeding human being, beyond its by-products, that offer great diversities of products for the nourishing industry. Between the main factors that limit the attainment of high incomes, are the illnesses caused by microorganism like fungi, bacteria, and viruses that, in general, are difficult to control and cause damages on harvest of billions of dollar every year. An alternative to minimize the losses is preserving the grains through the irradiation that can come today from two different main sources: e-beam and 60 Co. Beyond power to be off when it will not be in use, the source of e-beams machines does not need to be recharged, is easily available, possess high tax of dose and low energy. However the 60 Co have low dose rate, high energy and the photons emission is continuous. This work aims to compare the effects of the radiation through viscosimetry, DNA Comet Assay and Cooking time techniques in soybean grains at doses 0, 1.0, 3.0 and 5.0kGy irradiated in ambient temperature at 60 Co source Gammacell 220 (A.E.C. Ltda) and in e-beam accelerator - Radiation Dynamics (Radiation Dynamics Co. model JOB, New York, USA), 1.5 MeV- 25mA with the lower dose. (author)

  11. Hassall's corpuscles in the guinea-pig thymus after a second /sup 60/Co local irradiation

    Energy Technology Data Exchange (ETDEWEB)

    Kinoshita, H [Nara Medical Univ., Kashihara (Japan)

    1974-12-01

    The cervical regions of male guinea-pigs, weighing about 300 g, were irradiated locally with /sup 60/Co 800 R, and the same dose of irradiation was repeated a second time in each guinea pig at a different interval. Thereafter, an irradiated animal was sacrificed every other day for 2 weeks. Thymuses were weighed and fixed in 10 percent formal saline. Tissues were blocked in paraffin and each block was cut into serial sections having a thickness of approximately 6 microns. These sections were stained with the haematoxylin-eosin (HE) and the aldehyde-fuchsin (AF) techniques. The size of Hassall's corpuscles was measured in the sections of HE and the AF (+) ratio of Hassall's corpuscles was calculated in sections of AF. The ratio (%) of the number of AF (+) corpuscles to the total number of corpuscles seen in the entire microscopical field was called ''AF (+) ratio''. The weight of the twice irradiated thymuses decreased regardless of the interval from the first irradiation. The tendency of Hassall's corpuscles to enlarge during the period of enlargement of the corpuscles after first irradiation was increased by the second irradiation. The enlarged pattern of Hassall's corpuscles after a single irradiation of /sup 60/Co 800 R re-appeared when the animals were given a second irradiation on the 35th day. Concerning the AF (+) ratio of Hassall's corpuscles, the increasing pattern of AF (+) ratio such as after single irradiation of /sup 60/Co 800 R re-appeared when the animals were given a second irradiation on the 35th day, but no effect was observed during the process of enlargement and disintegration of Hassall's corpuscles after the second irradiation. The enlargement of Hassall's corpuscles is attributed not only to the process of lympholysis, but also to various other factors such as the number and size of the corpuscles and the stage in their life cycle.

  12. Scattering factor evaluation for internal dose assessment due to 60Co

    International Nuclear Information System (INIS)

    Gautam, Y.P.; Kumar, A.; Sharma, S.; Sharma, A.K.; Dube, B.; Hegde, A.G.

    2008-01-01

    Guidelines for the assessment of internal doses from monitoring suggest default measurement of uncertainties (i.e. lognormal scattering factor, SF) to be used for different types of monitoring data. In this paper, SF values have been evaluated for internal contamination due to 60 Co in two cases using whole body counting data. SF values of 1.04 and 1.03 were obtained for case I and II respectively while SF value of 1.03 was obtained using bioassay data for case I. SF evaluated is in good agreement with the default values given by IDEAS guidelines. (author)

  13. Viscosity of crystalline proteins in solution, when irradiated with 60 Co

    International Nuclear Information System (INIS)

    Bernardes, D.M.L.; Mastro, N.L. del

    1992-01-01

    In order to study 60 Co radiation effects on proteins, an aqueous solution of bovine crystalline was irradiated with doses from O to 25,000 Gy. Changes in viscosity were followed whether in the presence or absence of radiation response modifiers: glutathione (GSH), amino ethyl isothiourea (AET), mercapto ethyl alanine (MEA) e dimethyl sulfoxide (DMSO). Viscosity data at different temperature revealed that aggregate formation was the predominant process induced by radiation. The results showed also that in presence of those substances the radiation effects was diminished. (author)

  14. The effect of 60Co γ-rays irradiation on seeds and branches of Euonymus fortunei

    International Nuclear Information System (INIS)

    Ao Yan; Inner Mongolia Agriculture Univ., Huhhot; Zhang Guosheng; Lu Renqiang; Pan Qinghua

    2006-01-01

    Seeds and plant branches of Euonymus fortunei were irradiated with 60 Co γ-ray. The results showed that after irradiation, seed germination rate, emergence rate and surviving rate were restrained, and it was negatively correlated with irradiation dose increasing significantly. The first euphylis period was delayed compared with control. Seedling height decreased with the increase of irradiation dose. Semi-lethal dose of Euonymus fortunei seeds was 128.56 Gy. Branch irradiation could cause significantly number and length reduced, and it is correlated with irradiation dose increasing. The semi-lethal dose for Euonymus fortunei branches was 95.54 Gy. (authors)

  15. The 1998 calibration of Australian secondary standards of exposure and absorbed dose at 60Co

    International Nuclear Information System (INIS)

    Huntley, R.B.; Van der Gaast, H.

    1998-10-01

    New calibration factors are reported for several of the ionization chambers maintained at the Australian Radiation Laboratory (ARL) and at the Australian Nuclear Science and Technology Organisation (ANSTO) as Australian secondary standards of exposure/air kerma and absorbed dose at 60 Co. These calibration factors supplement or replace the calibration factors given in earlier reports. Updated 90 Sr reference source data are given for the ARL chambers, and for two of the ANSTO chambers. These results confirm the stability of the secondary standards. A re-calibration of the ANSTO reference electrometer is reported. This was carried out using an improved method, which is fully described

  16. Effect of 60Co-irradiation on penicillin G procaine in veterinary mastitis products

    International Nuclear Information System (INIS)

    Tsuji, K.; Goetz, J.F.; Vanmeter, W.

    1979-01-01

    The effect of 60 Co-irradation on penicillin G procaine in a peanut oil-based veterinary mastitis product was examined by reversed-phase high-performance liquid chromatography (HPLC). The HPLC method is capable of separating and quantifiying procaine, penicillin G, and various degradation compounds. Values obtained by the HPLC method on the product irradiated and stored at various temperatures correlated well with those of the microbiological assay. No significant decrease in the procaine was detected even after 4.0-Mrad irradiation. The HPLC method is applicable for analysis of other beta-lactam antibiotics

  17. The stem and leaf super green mutant induced by 60Co γ-rays irradiation

    International Nuclear Information System (INIS)

    Wei Yubo; Liang Naiting; Buhaliqiem; Zhang Yinbao

    2003-01-01

    Super green gene mutant was developed from population of M 2 generation after the dry seeds of rice Huazhiwu from Japan with good quality and resistance to cold had been irradiated with 50 Gy 60 Co γ-ray. The leaf, sheath, panicle axis and petiole of mutant was characterized by deeply green, and did not turn yellow after maturing date. The chlorophyll content in straw is 2.2 times higher than that in common straw. The results of raising livestock showed that horse, donkey and sheep had evident selectivity to the green straw

  18. Seasonal dynamics of 60Co uptake by freshwater algae under natural conditions

    International Nuclear Information System (INIS)

    Koulikov, N.V.; Trapeznikov, A.V.

    1988-08-01

    The data presented in the present report show that the values of 60 Co uptake coefficient in freshwater algae under naturel conditions can change 5-6 times depending on seasons, reaching maximum values in summer. Specific activity of the radionuclide in water can be essentially changed depending on the nuclear power plant operation mode. In such a nonequilibrium system it is rather questionable to use the uptake coefficient as a constant parameter for the determination of the radionuclide specific activity in water [fr

  19. Degradation of the photoluminescence of porous silicon caused by 60Co γ radiation

    International Nuclear Information System (INIS)

    Astrova, E.V.; Emtsev, V.V.; Lebedev, A.A.

    1995-01-01

    Two series of experiments were carried out. In the first, as-grown porous silicon was bombarded with 60 Co γ radiation to a dose ∼ 10 20 cm -2 . The photoluminescence intensity fell off by a factor ∼ 50 as a result, although the peak of the band underwent essentially no shift. In the second series, single-crystal silicon was bombarded to the same dose, and then porous silicon was fabricated on it. The intensity and spectra of these samples were the same as usual. Possible degradation mechanisms are discussed. 12 refs., 2 figs

  20. Radiation hardness evaluation of the commercial 150 nm CMOS process using 60Co source

    International Nuclear Information System (INIS)

    Carna, M; Havranek, M; Hejtmanek, M; Janoska, Z; Marcisovsky, M; Neue, G; Tomasek, L; Vrba, V

    2014-01-01

    We present a study of radiation effects on MOSFET transistors irradiated with a 60 Co source to a total absorbed dose of 1.5 Mrad. The transistor test structures were manufactured using a commercial 150 nm CMOS process and are composed of transistors of different types (NMOS and PMOS), dimensions and insulation from the bulk material by means of deep n-wells. We have observed a degradation of electrical characteristics of both PMOS and NMOS transistors, namely a large increase of the leakage current of the NMOS transistors after irradiation

  1. Ion exchange-based treatment of "6"0Co contaminated well-water for storing γ irradiation source

    International Nuclear Information System (INIS)

    Bi Meng; Miao Shilin; Zhang Xiaolu; Zhang Youjiu

    2014-01-01

    Objective: To select an efficient ion exchange resin to purify the "6"0Co contaminated well-water for storing radioactive source and to ensure the radioactivity of "6"0Co in treated well-water below 10 Bq/L. Methods: The radioactivity of "6"0Co in the water samples was measured by using the potassium cobaltinitrite coprecipitation-β counting method. The treatment efficiencies of two different ion exchange resins for the simulated "6"0Co-bearing waste water were compared to select a better one to dispose of the "6"0Co contaminated well-water. Results: The treatment efficiency of MBD-15-SC mixed ion exchange resin was about 5.8 times higher than ZGCNR50 strong-acid cation exchange resin. The radioactivity of "6"0Co in the contaminated well-water could be reduced from 4.16 × 10"5 Bq/L to 1.16 Bq/L by two-stage sorption of MBD-15-SC mixed ion exchange resin. Conclusions: Using several times of two-stage MBD-15-SC mixed ion exchange resin could effectively purify the "6"0Co contaminated well-water. The quality of the treated well-water could meet the sewage discharge standards. (authors)

  2. Development and evaluation of a technique for in vivo monitoring of {sup 60}Co in the lungs

    Energy Technology Data Exchange (ETDEWEB)

    Mello, J.Q. de; Lucena, E.A.; Dantas, A.L.A.; Dantas, B.M., E-mail: bmdantas@ird.gov.br [Instituto de Radioprotecao e Dosimetria (IRD/CNEN-RJ), Rio de Janeiro, RJ (Brazil)

    2015-07-01

    {sup 60}Co is a fission product of {sup 235}U and represents a risk of internal exposure of workers in nuclear power plants, especially those involved in the maintenance of potentially contaminated parts and equipment. The control of {sup 60}Co intake by inhalation can be performed through in vivo monitoring. This work describes the evaluation of a technique through the minimum detectable activity and the corresponding minimum detectable effective doses, based on biokinetic and dosimetric models of {sup 60}Co in the human body. The results allow to state that the technique is suitable either for monitoring of occupational exposures or evaluation of accidental intakes. (author)

  3. Detection and temporal variation of 60Co in the digestive glands of the common octopus, Octopus vulgaris, in the East China Sea

    International Nuclear Information System (INIS)

    Morita, Takami; Otosaka, Shigeyoshi; Fujimoto, Ken; Nishiuchi, Kou; Kimoto, Katsunori; Yamada, Haruya; Kasai, Hiromi; Minakawa, Masayuki; Yoshida, Katsuhiko

    2010-01-01

    60 Co were detected in common octopus specimens collected in the East China Sea in 1996-2005. The source of 60 Co has remained unclear yet. Stable isotope analyses showed that there was no difference in stable Co concentrations between octopus samples with 60 Co and without 60 Co. This result showed that the stable Co in the digestive gland of octopus potentially did not include a trace amount of 60 Co and the source of 60 Co existed independently. Furthermore, investigations of octopus in other area and other species indicated that the origin of the source of 60 Co occurred locally in the restricted area in the East China Sea and not in the coastal area of Japan. Concentrations of 60 Co have annually decreased with shorter half-life than the physical half-life. This decrease tendency suggests that the sources of 60 Co were identical and were temporary dumped into the East China Sea as a solid waste.

  4. Effects of 60Co γ-rays irradiation on seed growth of ground-cover chrysanthemum

    International Nuclear Information System (INIS)

    Ge Weiya; Wang Tiantian; Yang Shuhua; Zhao Ying; Ge Hong; Chen Lin

    2011-01-01

    The seeds of ground-cover chrysanthemum were used to study the effects of different doses of 60 Co γ-rays irradiation(10-50 Gy) on seed germination and physiological characteristics. The results showed that the rate of seed germination and seedling survival decreased significantly with the irradiation doses. With the increase of irradiation dose to above 20 Gy, the content of malondialdehyde (MDA) and activity of peroxidase (POD) in seedlings significantly increased. The similar trends were found in the activities of superoxide dismutase (SOD) and glutathione reductase (GR). Catalase (CAT) activity increased at doses lower than 20 Gy, and then decreased at the higher doses, whereas ascorbate peroxidase (APX) activity did not alter except for 40 Gy. It is concluded that the suitable irradiation dose of mutation breeding is 20 Gy for the seeds of ground-cover chrysanthemum. Although 60 Co γ-rays irradiation resulted in damage of membrane lipid peroxidation in the survival seedlings, the increased activity of CAT and POD could protect them against the damage. (authors)

  5. Change of cell cycle arrest of tumor cell lines after 60Co γ-irradiation

    International Nuclear Information System (INIS)

    Tang Yi; Liu Wenli; Zhou Jianfeng; Gao Qinglei; Wu Jianhong

    2003-01-01

    Objective: To observe the cell cycle arrest changes in peripheral blood mononuclear cells (PBMNCs) of normal persons and several kinds of tumor cell lines after 60 Co γ-irradiation. Methods: PBMNCs of normal persons, HL-60, K562, SiHA and 113 tumor cell lines were irradiated with 60 Co γ-rays at the absorbed doses of 6, 10,15 Gy. Cell cycles changes were checked 6, 12, 24, 48 and 60 h after the irradiation. Results: A stasis state was observed in normal person PBMNCs, 95 percents of which were in G 1 phase, and they still remained stasis after the irradiation. Except the 113 cell line manifesting G 1 phase arrest, all other tumor cell lines showed G 2 /M phase arrest after irradiation. The radiation sensitivity of HL-60 was higher than that of SiHA cell line. Conclusion: Different cell lines have different cell cycle arrest reaction to radiation and their radiation sensitivity are also different

  6. The effect of 60Co γ-rays on con A and LPS induced lymphocytes

    International Nuclear Information System (INIS)

    Su Liaoyuan; Liu Keliang; Ma Xiangrui

    1987-01-01

    The effect of 60 Co γ-rays on lymphocytes induced by Con A and LPS and the relationship between these two groups of cells were investigated by means of 3 H-TdR incorporation. The study showed that in vitro, Con A cells were able to promote the inducing effect of LPS to B cells. When Con A cells were irradiated by 10 Gy γ-rays, the 3 H-TdR incorporation value reduced significantly and the stimulating effect of Con A cells on LPS cells disappeared. Having been irradiated by γ-rays, LPS cells were not be able to be stimulated by normal Con A cells. When the groups of cells were incubated together after irradiation, the synergistic function disappeared, furthermore the suppressive effect of Con A cells on LPS cells emerged. When these two groups of cells were investigated by means of agar culture, the suppressive effect of 10 Gy γ-rays on lymphocytes colony formation was more obvious. Tests on 7 patients who were suffering from carcinoma of nasoparynx showed that after a course of treatment with 60 Co γ-rays, the incorporation value in Con A cells became much smaller, the stimulating effect of Con A cells on LPS cells disappeared. LPS cells could not be stimulated by normal Con A cells. The study demonstrated that the radiosensitivity of Con A cells is higher than that of LPS cells

  7. Effect of 60Co γ-rays on PWM and LPS induced lymphocytes

    International Nuclear Information System (INIS)

    Su Liaoyuan; Liu Keliang; Liu Fenju

    1987-01-01

    The relationship between lymphocytes induced by PWM (pokeweed mitogen) and LPS (lipopolysaccharide) was investigated by means of 3 H-TdR incorporation. The study showed that, in vitro, PWM-induced cells were able to promote the stimulating effect of LPS to B lymphocytes. The stimulating effect of PWM-induced cells was obviously weakened after PWM cells being irradiated with γ-rays. When PWM-induced cells and LPS-induced cells were incubated together, with one kind of cells exposed to 60 Co γ-ray, incorporation value of 3 H-TdR became much smaller and the synergetic function disappeared, especially, when PWM-induced cells were irradiated. For patients suffering from carcinoma of nasopharynx, while treated with 60 Co γ-rays, the incorporation value in LPS-induced cells approached normal level, meanwhile, the incorporation value in PEM-induced cells reduced significantly and the stimulating effect of PWM-induced cells on LPS-induced cells became much weaker. The facts described above demonstrated that PWM-induced cells have the function of T-helper cells and play more important role in the synergy than LPS-induced cells

  8. Effects of testicular irradiation with 60Co in guinea pigs CAVIA PORCELLUS

    International Nuclear Information System (INIS)

    Santos, O.R. dos.

    1980-01-01

    Improvements were made in restaint systems and in the technique of electroejaculation in guinea pigs, emphasizing safety, effectiveness and repetition. Arrangements for simultaneous testicular irradiation with 60 Co in 5 animals and a phantom to test dose levels in the target region and scattering were also made. Effects of single and fractionated exposures on sperm were studied. A collimated point-shaped 60 Co-machine calibrated to 7 R/min was used as radiation source. Groups of five 12 month aged male guinea pigs were exposed to either single testicular irradiation of 50, 100, 200 and 400 R or fractionated weekly testicular irradiation of 2, 5, 10 and 20 R, during 10 weeks. A last group was exposed to 4 fractionated testicular irradiations of 20 R at time intervals of 21 days and a group of 8 animals was used as control. Semen samples evaluations were made during 5 weeks before irradiation and in the subsequent 11 weeks. Abnormalities in sperm morphology were studied by William's method and phase contrast microscopy. Mean percentages of semen normal espermatzoons and spermatic abnormalities are shown in figures and photomicrographs. Marked differences on concentration, sperm motility and sperm abnormalities were found, but not on volume, in favour of 100 and 200 R single doses levels, as compared to fractionated 10 and 20 R. (Author) [pt

  9. Grafting of HEMA onto dopamine coated stainless steel by 60Co-γ irradiation method

    International Nuclear Information System (INIS)

    Jin, Wanqin; Yang, Liming; Yang, Wei; Chen, Bin; Chen, Jie

    2014-01-01

    A novel method for grafting of 2-hydroxyethyl methacrylate (HEMA) onto the surface of stainless steel (SS) was explored by using 60 Co-γ irradiation. The surface of SS was modified by coating of dopamine before radiation grafting. The grafting reaction was performed in a simultaneous irradiation condition. The chemical structures change of the surface before and after grafting was demonstrated by Fourier transform infrared (FTIR) spectrometer. The hydrophilicity of the samples was determined by water contact angle measurement in the comparison of the stainless steel in the conditions of pristine, dopamine coated and HEMA grafted. Surface morphology of the samples was characterized by atomic force microscope (AFM) and scanning electron microscope (SEM). The corrosion resistance properties of the samples were evaluated by Tafel polarization curve. The hemocompatibility of the samples were tested by platelet adhesion assay. - Highlights: • Poly-HEMA was grafted onto the surface of SS by 60 Co-γ-ray irradiation. • Pristine SS was coated by dopamine to form a dense poly-dopamine film before radiation grafting. • The biocompatibility and hydrophility of SS were improved after the grafting of HEMA

  10. Sorption behavior of human bone powder towards 60 Co and 65 Zn

    International Nuclear Information System (INIS)

    Abdel-Fattah, A.T.; Essa, M.W.A.; Mohamed, S.A.; Molokhia, M.K.

    1990-01-01

    Human bone powder 30-40 Μ in diameter was prepared from human bone femurs as fat-free (FFB), protein-free (PFB) or left untreated as raw bone powder (RB). The sorption behavior of human bone powder towards 60 Co and 65 Zn was studied. The uptake changed with the type of bone powder to be : PFB>FFB>RB. The increase in the concentration of cobalt(from 10 -6 to 10 -1 Mole/litre)and of zinc (from 10 -7 to 10 -4 M/1) increased the uptake of 60 Co and 65 Zn. Freunclich-type isotherm was successfully applied on the uptake data of both ions and the slopes of these isotherms were, nearly, directly proportional to their uptake values. The uptake was found to be less influenced by the PH. In case of cobalt the uptake increased till PH 4, followed by a plateau till PH 8 while in case of zinc the PH effect is much less pronounced

  11. Comparative kinetic analysis of89 Sr,60 Co and65 Zn Uptake by human bone powder

    International Nuclear Information System (INIS)

    Abdel-Fatah, A.T.A.; Essa, M.W.A.; Mohamed, S.A.; Molokhia, M.K.

    1990-01-01

    Human bone powder samples were prepared from recent femurs. The Bone particles range between 30 and 40 MU in diameter. One portion of this powder was prepared fat-free (FFB), the second portion as protein-free (PFB) and the last portion was left as raw bone powder-(RB). The sequence of uptake of 89 Sr by these types of bone powder is : FFB > RB > PFB, while that of 60 Co and 65 Zn is: PFB > FFB > RB. Kinetic analysis of the uptake curves of the 3 isotopes indicated that these processes proceed in 3 distinct steps; very fast initial, moderate intermediate and slow last step. The obtained rates of uptake indicated that : (1) the uptake by PEB is faster in its third step than the other types, (2) the most predominant step in case of 89 Sr and 60 Co is the third step (ion exchange step) while in case of 65 Zn it is the first step (physical adsorption), (3) defatenisation or deproteinisation, in general, inhances the uptake process

  12. Determination of distribution ratio for 60Co, 85Sr and 134Cs in loess medium

    International Nuclear Information System (INIS)

    Tanaka, Tadao; Ogawa, Hiromichi; Zhentang Li; Hui Wang; Kamiyama, Hideo.

    1994-06-01

    Distribution ratio R for 60 Co, 85 Sr and 134 Cs between loess and ground water was measured by using 16 loess samples taken from the field test site of China Institute for Radiation Protection, in which the field test of radionuclide migration had been performed. The R of each radionuclide was taken nearly constant value among 16 samples. This revealed that the site can be regarded as uniform loess medium from the view point of sorption ability. The sorption ability between the three radionuclides was the order of 134 Cs > 60 Co >> 85 Sr. The 85 Sr was an available nuclide for examining the migration phenomena, because 85 Sr might easily migrate in the loess medium due to its low sorption ability. The results of parametric check suggested that it is necessary to select the valid temperature and pH for determining the R value. The sorption of Sr on the loess was dominated by reversible ion-exchange reaction, while that of Co and Cs was dominated by irreversible one. (author)

  13. Ionizing radiation from 60Co and electron accelerator in reducing the population of Salmonella sp. inoculated in chicken meatballs: evaluation of acceptance by consumer

    International Nuclear Information System (INIS)

    Saito, Juliana

    2003-01-01

    Salmonella sp. is one of the main microorganisms that causes outbreaks of food borne diseases associated to poultry, and among its derivatives, the chicken meatballs are getting the favorites of the consumer. In the attempt to improve microbiological food safety, a method that has been hardly studied is the irradiation. To study the viability of the use o ionizing radiation originated from 60 Co and electrons accelerator in the reduction of Salmonella sp., frozen chickens meatballs were inoculated with 10 4 CFU/g of Salmonella sp., with needle and syringe. Subsequently they were exposure to doses of 1.5, 2.0, 2.5 and 3.0 kGy and Salmonella sp. was enumerated. Chicken meatballs were exposed to doses 3.0, 4.0 and 5.0 kGy and submitted to sensory analysis. The gamma radiation from 60 CO was effective in the reduction of Salmonella sp. inoculated in chicken meatballs. The ionizing radiation originated from electron beam was not effective in the conditions applied in this research. The commercial chicken meatballs prepared with chicken meat, mixed up with soy protein, seasoning and anti-oxidants additives did not loose their sensorial quality when exposure to doses of 3.0, 4.0 and 5.0 kGy and, in a general way, the consumers showed to be disposed to buy the irradiation product (author)

  14. Inhibition of MAPK and PKC pathways by 60Co γ-radiation in cultured vascular smooth muscle cells

    International Nuclear Information System (INIS)

    Jia Guanghong; Ma Yexin; Xiao Jianming

    2002-01-01

    Objective: To investigate the signal transduction pathways inhibited by 60 Co γ-radiation in cultured vascular smooth muscle cells (VSMC). Methods: The cultured VSMC were irradiated with 60 Co γ-radiation of 3.5, 7.0 and 14 Gy respectively. VSMC proliferation was measured by 3 H-TdR incorporation, while PKC, MAPK activities were determined by radioactivity assay. Results: Proliferation of VSMC was inhibited by 7.0, 14 Gy 60 Co γ-irradiation and the activities of PKC, MAPK were decreased significantly. Conclusion: Inhibitory effect of 7.0, 14 Gy 60 Co γ-irradiation on proliferation of VSMC might be resulted from decrease of the activity of PKC, MAPK

  15. Comparative retention of 60Co, 109Cd, and 137Cs following acute and chronic feeding in Bobwhite quail

    International Nuclear Information System (INIS)

    Anderson, S.H.; Dodson, G.J.; Van Hook, R.I.

    1975-01-01

    Tissue distribution of the radionuclides were found in the groups given the chronic exposure. Under chronic exposure to 60 Co, 109 Cd, and 137 Cs, quail rapidly accumulated these radioisotopes and the respective body burden concentrations began to level off after 11, 21, and 21 days of exposure. The biological half-lives for radioisotopes accumulated during the chronic feedings were 13 days for 60 Co, 8 days for 109 Cd, and 11 days for 137 Cs. In contrast, biological half-lives for these radioisotopes under acute feeding conditions (single four-hour feeding) were 15 days for 60 Co, 3 days for 109 Cd, and 10 days for 137 Cs. These results indicate that food chain behavior of 60 Co and 137 Cs in Bobwhite is similar following either acute or chronic exposure to contaminated food. For 109 Cd, biological retention in Bobwhite was longer under chronic exposure conditions. (U.S.)

  16. Measurements of 60Co in spoons activated by neutrons during the JCO criticality accident at Tokai-mura in 1999

    International Nuclear Information System (INIS)

    Gasparro, J.; Hult, M.; Komura, K.; Arnold, D.; Holmes, L.; Johnston, P.N.; Laubenstein, M.; Neumaier, S.; Reyss, J.-L.; Schillebeeckx, P.; Tagziria, H.; Van Britsom, G.; Vasselli, R.

    2004-01-01

    Neutron activated items from the vicinity of the place where the JCO criticality accident occurred have been used to determine the fluence of neutrons around the facility and in nearby residential areas. By using underground laboratories for measuring the activation products, it is possible to extend the study to also cover radionuclides with very low activities from long-lived radionuclides. The present study describes γ-ray spectrometry measurements undertaken in a range of underground laboratories for the purpose of measuring 60 Co more than 2 years after the criticality event. The measurements show that neutron fluence determined from 60 Co activity is in agreement with previous measurements using the short-lived radionuclides 51 Cr and 59 Fe. Limits on contamination of the samples with 60 Co are evaluated and shown to not greatly affect the utility of neutron fluence determinations using 60 Co activation

  17. sup(60)Co hot atom chemistry of tris(acetylacetonato) cobalt(III) adsorbed on silica gel

    International Nuclear Information System (INIS)

    Nishioji, H.; Sakai, Y.; Tominaga, T.

    1985-01-01

    The sup(60)Co hot atom reactions were studied in tris(acetylacetonato)cobalt(III) adsorbed on silica gel surface. sup(57)Fe Moessbauer spectra of tris(acetylacetonato)iron(III) in the corresponding system were also measured in order to examine the state of dispersion of complex molecules on silica gel. The retention formation processes were discussed in terms of the dependence of sup(60)Co retention on the adsorbed amount (concentration) of cobalt(III) complexes. (author)

  18. Screening of strains with the high activity and thermostability nattokinase by {sup 60}Co γ-ray irradiation

    Energy Technology Data Exchange (ETDEWEB)

    Shuying, Li; Ying, Nie; Huan, Du; Xuanming, Tang [Institute of Agro-products Processing Science and Technology, Chinese Academy of Agricultural Sciences, Beijing (China); Zhonglin, Zhao [College of Sciences, Henan Agricultural University, Zhengzhou (China); Xin, Ma [Agricultural Information Institute, Chinese Academic of Agricultural Sciences, Beijing (China); Yan, Li [School of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou (China)

    2013-06-15

    In this study, Bacillus natto was irradiated by {sup 60}Co γ-ray, and activity was determined by Casein plate method in order to get high activity and thermostability strains. 60 strains with high activity were obtained through irradiation by 800 Gy {sup 60}Co γ-ray. In this dose, the positive mutation rate was 45%. Then 60 strains was treated by different tempreture and 11 strains showed thermostability at 65℃. (authors)

  19. Screening of strains with the high activity and thermostability nattokinase by "6"0Co γ-ray irradiation

    International Nuclear Information System (INIS)

    Li Shuying; Nie Ying; Du Huan; Tang Xuanming; Zhao Zhonglin; Ma Xin; Li Yan

    2013-01-01

    In this study, Bacillus natto was irradiated by "6"0Co γ-ray, and activity was determined by Casein plate method in order to get high activity and thermostability strains. 60 strains with high activity were obtained through irradiation by 800 Gy "6"0Co γ-ray. In this dose, the positive mutation rate was 45%. Then 60 strains was treated by different tempreture and 11 strains showed thermostability at 65℃. (authors)

  20. Bio-geochemical behavior of 90Sr, 137Cs and 60Co in tropical soil

    International Nuclear Information System (INIS)

    Wasserman, M.A.; Gonzales Viana, A.; Bartoly, F.; Carvalho Conti, C. de; Janvrot Vivone, R.; Perez, V.; Wasserman, J.C.

    2004-01-01

    One manner to assess the bio-geochemical behavior of radionuclides in soils is the integration of experimental methods results obtained in the laboratory with results obtained in field experiments. In this work was used an alternative sequential chemical extraction protocol to evaluate mobility of radionuclides as a function of some physico-chemical conditions operationally defined: Slightly acidic phase: CH 3 COOH + CH 3 COONa 1:1, pH 4.7, shacked at room temperature; Easily reducible phase: NH 2 OH.HCl (0.1 M), pH 2, shacked at room temperature;Oxidizable phase: H 2 O 2 (30%) + HNO 3 , pH 2, CH 3 COONH 4 (1 M);shacked at room temperature; Alkaline phase: NaOH (0.1 M), pH 12, shacked at room temperature; Resistant phase: Aqua regia heated to 50 deg C / 30 min. The results obtained experimentally indicate the vulnerability of some Brazilian soils due to the higher radionuclide transfer to plant. Although it seems clear that it is difficult to identify which soil property will determine a given TF, the results of geochemical partition for 137 Cs, 90 Sr and 60 Co obtained in slightly acidic phase were very consistent with the TF data for reference plants or with some of the soil properties recognized in the specialized literature as related with mechanisms of sorption of Cs (e.g. exchangeable K, organic matter and iron oxides content), Co (e.g. manganese oxide) and Sr (pH and exchangeable Ca). The 137 Cs distribution in soil showed that Fe oxides are the main sink for this element in all type of soil and 16 years after contamination the 137 Cs the TF remains almost the same in Goiania soil. The 60 Co distribution in soil showed that Mn oxides are the main sink for this element in all type of soils and in the Nitisol, 5 years after contamination, the 60 Co was not detected as bioavailable (in the slightly acidic phase) neither detected in plant or were detectable with values very close to the detection limit. In the Nitisol, it is possible that reduction in 137 Cs

  1. Measurement of disintegration rates of 60Co volume sources by the sum-peak method

    International Nuclear Information System (INIS)

    Kawano, Takao; Ebihara, Hiroshi

    1991-01-01

    The sum-peak method has been applied to the determination of the disintegration rates of 60 Co volume sources (1.05 x 10 4 Bq, 1.05 x 10 3 Bq and 1.05 x 10 2 Bq, in 100-ml polyethylene bottles) by using a NaI(Tl) detector of a diameter of 50 mm and a height of 50 mm. The experimental results showed that decreasing the disintegration rates resulted in enlarged underestimation in comparison with the true disintegration rates. It was presumed that the underestimations of the disintegration rates determined by the sum-peak method resulted from the overestimations of the areas under the sum peaks caused by the overlap of the area under the Compton scattering of the γ-ray (2614 keV) emitted from a naturally occurring radionuclide 208 Tl under the sum peaks. (author)

  2. Tracer diffusion of 60Co and 63Ni in amorphous NiZr alloy

    International Nuclear Information System (INIS)

    Hoshino, K.; Averback, R.S.; Hahn, H.; Rothman, S.J.

    1987-01-01

    Tracer diffusion of 60 Co and 63 Ni in equiatomic amorphous NiZr alloy in the temperature range between 486 and 641 0 K can be described by: D/sub Co/sup */ = 3.7 x 10 -7 exp[-(135 +- 14) kJ mole -1 /RT] m 2 /sec and D/sub Ni//sup */ = 1.7 x 10 -7 exp[-(140 +- 9) kJ mole -1 /RT] m 2 /sec. The values of D/sub Ni//sup */ are in reasonable agreement with those measured by the Rutherford backscattering technique. The measured diffusivities were independent of time, indicating that no relaxation took place during diffusion. 27 refs., 2 tabs

  3. Effects of 60Co γ-rays irradiation on germination and seedling growth of Hibiscus syriacus

    International Nuclear Information System (INIS)

    Li Xiufen; Wu Fulan; Zhang Deshun; Meng Zhennong; Cao Jiyun

    2009-01-01

    The seeds of Hibiscus syria cus were irradiated by 60 Co γ-rays at dose rate of 50 ∼ 500Gy/h for 0.5 and 1h, the seed germination rate and the seedling growth characters were surveyed. The results indicated that the seed germination was promoted when the absorbed dose was below 200Gy, and the seedling survival rate was increased when the dose was below 100Gy. The germination was inhibited, leaves and secondary roots were difficult to form, and the seedlings blasting occurred when the dose was over 200Gy. The dose rate affects the irradiation results. Absorbed dosed of 50 ∼ 100Gy were suggested for increasing seedling growth rate and 100 ∼ 200Gy were recommend for mutagenesis. (authors)

  4. Nitroxyl free radicals formed from hindered amine light stabilizers under 60Co γ-ray irradiation

    International Nuclear Information System (INIS)

    Wang Huiliang; Chen Wenxiu

    2006-01-01

    Nitroxyl free radicals formed from several low molecular weight (LMW) hindered amine light stabilizers (HALS) under 60 Co γ-ray irradiation was studied with electron spin resonance (ESR) spectroscopy. All the HALSs irradiated in air formed nitroxyl free radicals under irradiation in air. For most of the HALSs, concentration of the nitroxyl free radicals increased linearly and quickly with absorbed dose in 0-10 kGy range, but increased slowly, or even kept constant, with doses of greater than 10 kGy. Concentration of nitroxyl free radicals formed from LMW HALS was usually higher than high molecular weight HALS. Tetramethyl HALS was easier to form nitroxyl free radicals than pentamethyl HLAS. Concentration of nitroxyl free radicals formed from the samples irradiated in oxygen was about two times higher than that the samples irradiated in air. Mechanisms of the nitroxyl free radical formation from the γ-ray irradiated HALSs were was discussed. (authors)

  5. The study of mildew proof effect of 60Co γ irradiation on leather shoes

    International Nuclear Information System (INIS)

    Wu Caixuan; Deng Zhiqun; Yu Qin; Zeng Daonan; Deng Guili

    1992-01-01

    Leather products occupy an important place in the national economy. In order to prevent from mildewing of leather products in storage, transportation and sale, the packed shoes were irradiated with 60 Co γ ray. Test materials are cattle, sheep and pig leather shoes. The results shows that: 1. There are 11 types of molds which make leather shoes mildewed: Oospora sp, Rhizopus oryzae Went and Grrelings, Aspergillus flavus Link, Aspergillus terreus thom, Monilia sp, Botrytis sp, Aspergillus niger V. Tiegh, Penicillum Lividum Westling, Penicillum Corylophilum Dieckx, Penicillum Cyaneum (B. and S.) Biourge and Penicillum Verrulosum Peeyrone. 2. The three types of shoes packed with polyethylene film can be prevented from mildewing after irradiation at level of 12 kGy. 3. The irradiation at 30 kGy does not change the physical properties of the shoes. 4. The irradiated shoes packed with polyethylene film can prevent mildewing, but unpacked ones would be mildewed again

  6. Radiation sensitivity of noise in monolithic JFET circuits exposed to 60Co γ - rays

    International Nuclear Information System (INIS)

    Cesura, G.; Re, V.; Tomasini, A.

    1993-01-01

    The spectral density of channel current noise in JFET's is governed in the low-frequency region by Lorentzian terms. Ordinarily, their influence on the equivalent noise charge of preamplifiers for radiation detectors is negligible, as these terms vanish as soon as the frequency exceeds a few Hz. Exposure of JFET's to γ - rays has proved, however, that these noise contributions are enhanced by the absorbed radiation and may invade a much broader frequency range, thereby degrading the equivalent noise charge even at comparatively short processing times. The extent to which the noise behaviour of JFET's is affected by radiation-enhanced Lorentzian terms is analysed in this paper. Results of measurements, carried out after exposure to 60 Co γ - rays of JFET parts and monolithic circuits realised in two different technologies, are discussed and compared with the theoretical predictions. (orig.)

  7. Reliability of planar silicon transistors exposed to 60Co γ rays

    International Nuclear Information System (INIS)

    Blin, A.; Le Ber, J.

    1966-01-01

    This report gives an account of results obtained during investigations on the reliability of silicon Planar Transistors, irradiated by the 60 Co γ rays. We consider in a first part the variation of the average values of the parameters of the lots under test. Then, a more complete statistical study is carried out (distribution of the values of the parameters within the lots; research of correlations, etc. ). It is clearly stated and shown that evaluation of the degradation of the gain of transistors depends on: the conditions of measurement (voltage, current), after irradiation; the polarisation of the elements during irradiation; the origin of manufacture of the lots under test (4 manufacturers). We show then the difficulties met to predict the behaviour of the transistors under radiation stress, and attempt is made to define practical rules for design engineers. (author) [fr

  8. Retention of 60Co, 85Sr and 137Cs on inorganic ion exchangers

    International Nuclear Information System (INIS)

    Dozol, J.F.; Eymard, S.

    1983-11-01

    The aim of the study is the treatment of radioactive wastes produced in plutonium fuel fabrication or in spent fuel reprocessing by inorganic ion exchangers for ultimate storage. This rapport, gives the distribution coefficients of 60 Co, 85 Sr, 137 Cs (in sodium nitrate medium at different concentration of sodium: .23g/l, 1 g/l, 10 g/l) obtained with different inorganic exchangers: titanium oxyde, sodium titanate, sodium zirconate, sodium niobate, sodium tantalate, titanium phosphate, zirconium phosphate, ammonium phosphotungstate in zirconium phosphate, polyantimonic acid amorphous aluminosilicate and several zeolites (ZBS 15 from OXYMIN, ZEOLON 400, ZEOLON 500, ZEOLON 900 from Norton, IE 96, A 51, 13 X from Union Carbide) [fr

  9. Biological effects of 60Co γ-irradiation on Laiwu ginger VM1 growth

    International Nuclear Information System (INIS)

    Zhou Ming; Huang Jinli; Wei Yuxia; Guan Qiuzhu; Zhang Zhenxian

    2008-01-01

    Rhizome of Laiwu ginger were treated with γ-irradiation at the doses of 0, 20, 40 and 60 Gy. The results showed that 60 Co γ-irradiation inhibited the rhizome burgeoning, and decreased the survival rate of the seedlings, rate of leaf- expansion and the growth of plants (VM 1 ). The inhibition effects became stronger with the increase of the irradiation dose. Different bands were found through the analysis of POD, EST isozymes and RAPD of VM 1 plants, which showed that variation on molecular level occurred in VM 1 plants. LD 30-40 was appropriate for the irradiation of rhizomes of Laiwu ginger and the optimal irradiation dose was about 20- 30 Gy. (authors)

  10. Combined effects of 60Co dose and high frequency interferences on a discrete bipolar transistor

    International Nuclear Information System (INIS)

    Doridant, A.; Raoult, J.; Jarrix, S.; Blain, A.; Dusseau, L.; Hoffmann, P.; Chatry, N.; Calvel, P.

    2012-01-01

    This paper concerns bipolar transistors subject to a double aggression: dose irradiation and high-frequency interference. The electromagnetic interference is injected in a contactless way in the near-field zone around the device. Parameters of the interference are power and frequency, the latter largely out of band of operation of the transistors. The output voltage of the transistor exhibits changes, due to rectification and to some extent to current crowding. The importance of the base bias set-up for the type of change occurring in voltage is displayed. After irradiation with a 60 Co source, the voltage output will change under electromagnetic interference but sometimes in an opposite way as initially measured. The impact of the irradiation with respect to electromagnetic susceptibility is highlighted from a physical point of view. Finally preliminary results of simulation for susceptibility prediction are given and a discussion is given on the limits of the transistor model used. (authors)

  11. Case of radiation gastroduodenitis caused by /sup 60/Co-irradiation therapy for hepatoma

    Energy Technology Data Exchange (ETDEWEB)

    Nishikawa, H; Hayashi, N; Morise, K; Tunekawa, J; Kaneshiro, K [Nagoya Univ. (Japan). Faculty of Medicine

    1981-02-01

    A 56-year-old man with hepatoma, who had been treated with total 3,960 rad of /sup 60/Co-irradiation 2 months previously, was readmitted to the hospital because of fever and anemia. Following admission, he passed tarry stools every day. Barium meal examination revealed esophageal varices and erosive gastritis of the antrum. At endoscopy, many hemorrhagic erosions were found in the gastric antrum and the first part of duodenum, which were located in the irradiation area. Since repeated blood transfusion failed to improve anemia, a complete fasting with intravenous hyperalimentation and antacid therapy were started. Two months later, feeding was started and thereafter continued without any appreciable GI bleeding or worsening of anemia. Endoscopic examination at this time revealed only a few erosions scattered over the edematous antral mucosa as well as the proximal duodenum. IVH, antacids and abstinence from food seem to be an effective measure in the treatment of radiation injury of the gut.

  12. A preliminary study on doses of 60Co-γ ray for mutation breeding of Roegneria

    International Nuclear Information System (INIS)

    Jiang Yun; Lin Yang; Xuan Pu; Luo Xiaomei; Zhang Haiqin

    2011-01-01

    Six groups from three species of Roegneria were radiated with eight ladders of 60 Co-γ ray for finding the most suitable absorbed dose. The dose ladders were CK (0 Gy), 50 Gy, 100 Gy, 150 Gy, 200 Gy, 250 Gy, 300 Gy and 400 Gy. The half-lethal dose of six gourps, generated by multi-Target Single Hit Model Equation, were from 59.6 Gy to 172.8 Gy. Germination percentage of seeds, height of seeding, plant survival percentage and setting percentage were investigated. The most suitable absorbed doses of each species primerly were deduced from these data. The research provide a valuable reference for Roegneria mutation breeding on chousing the absorbed does. (authors)

  13. Effects of imidazole derivatives in the survival of 60Co irradiated mice

    International Nuclear Information System (INIS)

    Villavicencio, A.L.C.H.; Mastro, N.L. del.

    1988-07-01

    The presence of hypoxic and radioresistant cells is considered the main reason of failure in radiotherapy of neoplasms. Hypoxic cell radiosensitizers, as nitroimidazole derivatives, have an advantage over other alternative methods for improving the effects of radiotherapy since hypoxic cells exist in considerable concentration in tumours and only in small concentration in normal tissues. Its show also a direct cytotoxicity over the hypoxic cell population. In this work, studies on combining ip administered drugs and single dose radiation treatments in healthy albino mice are presented. It was compared the action of 2-nitroimidazole, levamisole and cysteine, the latest considered as radioprotector for several biological systems. The results showed some radioprotective action for 2 - nitroimidazole (MISO), sensitizer capacity for levamisole and in those conditions, cysteine failed to produce any effects on the survival of 9 Gy 60 Co irradiated mice. (author) [pt

  14. Effect of 60Co γ-irradiation on saccharification of uncooked sweet potato material

    International Nuclear Information System (INIS)

    Hu Tingchun; Xiong Xingyao; Yi Jinqiong; Wang Keqin; Su Xiaojun; Zou Jianfeng

    2010-01-01

    Using the starch and powder of sweet potato of Xiangshu 86 and Xiangshu 541 as materials, the effect of 60 Co γ-irradiation on the structure of starch particle and the efficiency of saccharification were studied. The result showed that some reticulate flaws appeared in the surface of irradiated starch particles, and the reticulate flaws were increased with the increase of irradiation dose. The content of reducing sugar and total soluble sugar in both starch and the powder were obviously increased along with the increase of irradiation dose ranged from 50 to 1200 kGy. The saccharification efficiency of Xiangshu 86 and Xiangshu 541 was obviously difference at the dose lower than 500 kGy, and then the efficiency showed the similar trends at higher dose irradiation, the saccharification rate reached the highest value after the treatment of 1200 kGy irradiation. (authors)

  15. Half-lives of isomeric levels of 107mAg, 109mAg and 103mRh photoactivated by 60Co γ-ray irradiation

    International Nuclear Information System (INIS)

    Yoshida, Eiji; Kobayashi, Takashige; Kojima, Yasuaki; Shizuma, Kiyoshi

    2000-01-01

    Photoactivation by γ-rays from 60 Co of 10 kCi has been performed for isomers of 107m Ag, 109m Ag and 103m Rh and half-lives of these isomers were determined. Gamma-rays emitted from 107m Ag and 109m Ag were measured with a low-background Ge detector and internal conversion electrons from 103m Rh were measured with a 2π gas flow counter. The half-lives obtained are: 107m Ag: 44.5±0.8 s, 109m Ag: 38.0±1.2 s and 103m Rh: 54.8±3.8 min. The results are in agreement with previous values obtained by different excitation methods

  16. Comparison of the standards for absorbed dose to water of the VNIIFTRI, Russia and the BIPM in 60Co γ rays

    International Nuclear Information System (INIS)

    Allisy-Roberts, P.J.; Kessler, C.; Burns, D.T.; Berlyand, V.; Berlyand, A.

    2010-01-01

    A new comparison of the standards for absorbed dose to water of the All-Russian Scientific Research Institute for Physical-Technical and Radio-technical Measurements (VNIIFTRI), Russia and of the Bureau International des Poids et Mesures (BIPM) has been made in 60 Co gamma radiation in 2009. The results show that the VNIIFTRI and the BIPM standards for absorbed dose to water are in agreement, yielding a mean ratio of 0.9976 for the calibration coefficients of the transfer chambers, the difference from unity being within the combined standard uncertainty (0.0043) for this result. This result is consistent with the earlier 2001 comparison result of 0.9967 (43). The updated degrees of equivalence for the VNIIFTRI are compared with those of the other national metrology institutes as presented in the BIPM key comparison database. (authors)

  17. Phenotype and molecular analysis of M1 generation of stylosanthes irradiated by 60Co

    International Nuclear Information System (INIS)

    Zhang Weili; Liu Fengmin

    2012-01-01

    The seeds of Stylosanthes guianensis SW. ‘Reyan No.13’ were irradiated by 60 Co at 0, 325, 487 and 974 Gy. Phenotype and molecular variations of Stylosanthes M 1 generations were studied. Germination percentage, plant height, leaf length and stem diameter of M 1 seedlings were determined using SRAP-PCR. Results showed that biological character indices of treatments were significantly lower than that of control (CK). Irradiation inhibited plant growth. Growth indices of 974 Gy treatment, except leaf length, were significantly lower than CK and other two treatments. Eight effective primers selected from 24 primers combination were used for SRAP-PCR. SRAP analysis revealed that the 57 of 88 DNA fragments were amplified showing polymorphisms. The average percentage of polymorphic bands was 64.77%. The effects of 60 Coγ irradiation on DNA variation of seedlings were varied from different treatments. Numbers of polymorphic bands changed after irradiation. Coefficient of variation was in direct proportion to irradiation doses. Variation rates of 325 Gy, 487 Gy and 974 Gy treatments were 22.0%, 38.1% and 41.5%, respectively. The Nei’s genetic similarity coefficient of all treatments was ranged from 0.585 to 0.780 by software NTSYSpc 2.1 based on SRAP results and the average Nei's coefficient was 0.678. The Nei’s genetic similarity coefficient of 974 Gy treatment was 0.585 and variation degree was highest among all treatments, 487 Gy treatment was second and 325 Gy treatment was the lowest one. Based on present bands, four irradiated treatments were classified into two major groups by UPGMA cluster analysis. Group 1 included CK, 325 Gy and 487 Gy treatment and group 2 included 974 Gy. SRAP analysis determined variation of Stylosanthes after irradiation and the results of SRAP analysis were consistent with their biological indexes in some extent

  18. Study for optimization of a 60 Co industrial irradiator of 250 k Ci

    International Nuclear Information System (INIS)

    Pina V, G.

    1995-01-01

    This work presents results of a benchmark between calculated and experimental absorbed dose values for a typical product, in a 60 Co industrial irradiator, located at ININ, Mexico. The goal of this work is to reach a ± 10 % approximation between both values. ININ 60 Co industrial irradiator is a two level, two layer system with overlapping product configuration, 56 irradiation positions with an activity of around 250 k Ci. Calculated values were obtained by QAD-CGGP code. This code uses a point kernel technique, build-up factors fitting was done by geometrical progression and combinatorial geometry was used for 3 D system description. Main code modifications were related with source simulation by punctual sources and energy spectrum (16 energy groups) and anisotropic emission were also considered. Experimental data were obtained from routine dosimetry which was done with red acrylic pellets; they were irradiated together the product in predetermined positions, for 36 maximum and minimum absorbed dose values. Typical product was polypropylene Petri dish packages, apparent density 0.13 g/cm 3 . It was chosen because regular geometry (2 packages per full irradiator container) and enough amount for considering homogeneous loading inside irradiation chamber. Required minimum dose was 15 kGy. Results showed a 8% variation between calculated and experimental values for maximum absorbed dose (18.2 kGy vs 16.8 kGy) and 3 % variation for minimum absorbed dose (13.8 kGy vs 14.3 kGy); these results fixed with the original proposal. (Author)

  19. Quality of Intensity Modulated Radiation Therapy Treatment Plans Using a 60Co Magnetic Resonance Image Guidance Radiation Therapy System

    International Nuclear Information System (INIS)

    Wooten, H. Omar; Green, Olga; Yang, Min; DeWees, Todd; Kashani, Rojano; Olsen, Jeff; Michalski, Jeff; Yang, Deshan; Tanderup, Kari; Hu, Yanle; Li, H. Harold; Mutic, Sasa

    2015-01-01

    Purpose: This work describes a commercial treatment planning system, its technical features, and its capabilities for creating 60 Co intensity modulated radiation therapy (IMRT) treatment plans for a magnetic resonance image guidance radiation therapy (MR-IGRT) system. Methods and Materials: The ViewRay treatment planning system (Oakwood Village, OH) was used to create 60 Co IMRT treatment plans for 33 cancer patients with disease in the abdominal, pelvic, thorax, and head and neck regions using physician-specified patient-specific target coverage and organ at risk (OAR) objectives. Backup plans using a third-party linear accelerator (linac)-based planning system were also created. Plans were evaluated by attending physicians and approved for treatment. The 60 Co and linac plans were compared by evaluating conformity numbers (CN) with 100% and 95% of prescription reference doses and heterogeneity indices (HI) for planning target volumes (PTVs) and maximum, mean, and dose-volume histogram (DVH) values for OARs. Results: All 60 Co IMRT plans achieved PTV coverage and OAR sparing that were similar to linac plans. PTV conformity for 60 Co was within <1% and 3% of linac plans for 100% and 95% prescription reference isodoses, respectively, and heterogeneity was on average 4% greater. Comparisons of OAR mean dose showed generally better sparing with linac plans in the low-dose range <20 Gy, but comparable sparing for organs with mean doses >20 Gy. The mean doses for all 60 Co plan OARs were within clinical tolerances. Conclusions: A commercial 60 Co MR-IGRT device can produce highly conformal IMRT treatment plans similar in quality to linac IMRT for a variety of disease sites. Additional work is in progress to evaluate the clinical benefit of other novel features of this MR-IGRT system

  20. Influence of humic acid on migration of 60Co, 85Sr and 137Cs in coastal sandy soil

    International Nuclear Information System (INIS)

    Tanaka, Tadao; Ni Shiwei.

    1993-10-01

    Sorption and migration experiments were performed by both batch and column methods, to study influence of humic acid complexing on the mobility of 60 Co, 85 Sr and 137 Cs in a coastal sandy soil. The study focuses on a correlation between molecular-weight of dissolved humic acid and ability of the complexing with the radionuclides in liquid phase. Dissolved humic acid was complexed with 60 Co and 85 Sr. The interaction ability of 60 Co was independent of molecular-weight of humic acid, while that of 85 Sr depended on. The mobility of 60 Co increased under the condition with coexistence of humic acid as well as its sorption ratio decreased. The distribution profile of molecular-weight of 60 Co was kept at a constant in the solution before and after the sorption experiment, due to completing the sorption and complexation equilibrium. The mobility of 85 Sr increased under the condition with coexistence of humic acid, in spite of the sorption ratio of 85 Sr was not affected by the coexistence. Such contradiction was caused by different kinetics between the batch and column methods. The sorption ability and mobility of 137 Cs were not affected by the coexistence of the humic acid. (author)

  1. {sup 137}Cs, {sup 60}Co and {sup 40}K uptake by lettuce plants in two distributions of soil contamination

    Energy Technology Data Exchange (ETDEWEB)

    Quinto, Francesca [Dipartimento di Scienze Ambientali, Seconda Universita di Napoli, via Vivaldi 43, 81100 Caserta (Italy); CIRCE, INNOVA, via Campi Flegrei 34, Pozzuoli 80078 (Italy)], E-mail: francesca.quinto@unina2.it; Sabbarese, Carlo; Visciano, Lidianna; Terrasi, Filippo; D' Onofrio, Antonio [Dipartimento di Scienze Ambientali, Seconda Universita di Napoli, via Vivaldi 43, 81100 Caserta (Italy); CIRCE, INNOVA, via Campi Flegrei 34, Pozzuoli 80078 (Italy)

    2009-08-15

    {sup 137}Cs and {sup 60}Co, two of the radionuclides more representative of discharges from nuclear facilities, are of interest for radiological protections because of their great mobility in biosphere and affinity with biological systems. The aim of the present work is the investigation of the possible influence of the vertical distribution of {sup 137}Cs and {sup 60}Co in soil upon their uptake by lettuce as function of plant's growth. An experiment ad hoc has been carried out in field conditions. The results show that (i) the transfer of {sup 137}Cs and {sup 60}Co from soil to lettuce is independent by their distribution in soil, (ii) the soil-plant transfer factors of {sup 137}Cs and {sup 60}Co show a similar trend vs. growth stage, (iii) the {sup 40}K transfer factor trend is different from those of anthropogenic radionuclides, and (iv) {sup 137}Cs and {sup 60}Co specific activities are about 1 Bq/kg, in the mature vegetable with soil activity from 9 to 21 kBq/m{sup 2}.

  2. 137Cs, 60Co and 40K uptake by lettuce plants in two distributions of soil contamination

    International Nuclear Information System (INIS)

    Quinto, Francesca; Sabbarese, Carlo; Visciano, Lidianna; Terrasi, Filippo; D'Onofrio, Antonio

    2009-01-01

    137 Cs and 60 Co, two of the radionuclides more representative of discharges from nuclear facilities, are of interest for radiological protections because of their great mobility in biosphere and affinity with biological systems. The aim of the present work is the investigation of the possible influence of the vertical distribution of 137 Cs and 60 Co in soil upon their uptake by lettuce as function of plant's growth. An experiment ad hoc has been carried out in field conditions. The results show that (i) the transfer of 137 Cs and 60 Co from soil to lettuce is independent by their distribution in soil, (ii) the soil-plant transfer factors of 137 Cs and 60 Co show a similar trend vs. growth stage, (iii) the 40 K transfer factor trend is different from those of anthropogenic radionuclides, and (iv) 137 Cs and 60 Co specific activities are about 1 Bq/kg, in the mature vegetable with soil activity from 9 to 21 kBq/m 2 .

  3. 60 Co accumulation by scenedesnus obliquus. Mechanism of uptake and relative contribution of radionuclide adsorption and absorption

    International Nuclear Information System (INIS)

    Nucho, R.; Baudin, J.P.

    1989-06-01

    In this paper are presented the results of various 60 Co accumulation and depuration experiments, carried out to assess the respective contributions of passive and active processes in the radionuclide uptake by Scenedesmus obliquus. The experiments were conduced on living illuminated cells, living cells maintained in darkness and dead cells. Exposure study shows that living illuminated cells and dead cells incorporated the same amount of available 60 Co, in the order of 65%. In contrast, the radionuclide uptake by cells in darkness was very lower. Depuration experiments show that radiocobalt release by living illuminated cells and dead cells is an exponential process including two phases. The first, during which the decrease of the radioactivity was about 80%, corresponds to elimination of the 60 Co adsorbed on the cell walls. During the second phase, the slight decrease of the radioactivity results from the intracellular 60 Co desorption. The addition of EDTA in the depuration culture medium results in an almost instantaneous loss of 80% of the accumulated radionuclide. The results clearly indicate that 60 Co uptake is mainly a passive phenomenon, since adsorption accounts for 80% of the accumulation and passive diffusion for about 10%. Metabolic assimilation contributes for a weak part [fr

  4. 137Cs, 60Co and 125I bioaccumulation by seaweeds from the Angra dos Reis nuclear power plant region

    International Nuclear Information System (INIS)

    Guimaraes, J.R.; Penna-Franca, E.

    1985-01-01

    As part of a broad research program on the behaviour of critical radionuclides to be discharged into the sea by the first Brazilian nuclear power plant, the uptake, accumulation and loss of 137 Cs, 60 Co and 125 I by locally abundant seaweed species was studied. Uptake in static 12 liter aquarium experiments reached apparent steady-state in 2 to 7 days ( 60 Co and 125 I), or 2 to 3 weeks ( 137 Cs). Elimination followed a reverse pattern, being comparatively fast for 137 Cs and slow for 60 Co and 125 I. Dry weight bioaccumulation factors (BFs) were variable, falling in the 10 1 range for 137 Cs, 10 3 for 125 I and 10 3 to 10 4 for 60 Co. Various short-term experiments, performed over a 16 month period, showed marked temporal variations of 60 Co BFs for all species. The results demonstrated that the studied species may play an important role in the transfer of the critical radionuclides through local food webs and can be employed as useful monitors for routine or accidental radionuclide releases. (author)

  5. Biological dosimetry in radiological protection: dose response curves elaboration for 60Co and 137Cs

    International Nuclear Information System (INIS)

    Silva, Marcia Augusta da

    1997-01-01

    Ionizing radiation sources for pacific uses are being extensively utilized by modern society and the applications of these sources have raised the probability of the occurrence of accidents. The accidental exposition to radiation creates a necessity of the development of methods to evaluate dose quantity. This data could be obtained by the measurement of damage caused by radiation in the exposed person. The radiation dose can be estimated in exposed persons through physical methods (physical dosimetry) but the biological methods can't be dispensed, and among them, the cytogenetic one that makes use of chromosome aberrations (dicentric and centric ring) formed in peripheral blood lymphocytes (PBL) exposed to ionizing radiation. This method correlates the frequency of radioinduced aberrations with the estimated absorbed dose, as in vitro as in vivo, which is called cytogenetic dosimetry. By the introduction of improved new techniques in culture, in the interpretation of aberrations in the different analysers of slides and by the adoption of different statistical programs to analyse the data, significant differences are observed among laboratories in dose-response curves (calibration curves). The estimation of absorbed dose utilizing other laboratory calibration curves may introduce some uncertainties, so the International Atomic Energy Agency (IAEA) advises that each laboratory elaborates your own dose-response curve for cytogenetic dosimetry. The results were obtained from peripheral blood lymphocytes of the healthy and no-smoking donors exposed to 60 Co and 137 Cs radiation, with dose rate of 5 cGy.min. -1 . Six points of dose were determined 20,50,100,200,300,400 cGy and the control not irradiated. The analysed aberrations were of chromosomic type, dicentric and centric ring. The dose response curve for dicentrics were obtained by frequencies weighted in liner-quadratic mathematic model and the equation resulted were for 60 Co: Y = (3 46 +- 2.14)10 -4 cGy -1 + (3

  6. The effect of gamma 60Co radiation on the decomposition and dimers formation of γ-tocopherol

    International Nuclear Information System (INIS)

    Jasinska-Stepniak, A.

    1995-01-01

    A standard of γ-tocopherol (MERCK) was irradiated with the doses up to 20 kGy. The irradiation was performed using tocopherol 'in substantia' and dissolved in ethanol and benzene. Radiolytic decomposition and formation of dimers were determined using the Emmerie-Engel procedure. The yield of decomposition of γ-tocopherol does not indicate its particular sensitivity to the radiation treatments, since the most frequent G-values of chemical food components are 2-3. The concentration remained the detection level. (author)

  7. Free radicals in pyrimidines: ESR of. gamma. -irradiated 5-cyclohexenyl-1,5-dimethyl barbituric acid. [/sup 60/Co

    Energy Technology Data Exchange (ETDEWEB)

    Benson, B. (Lehigh Univ., Bethlehem, PA); Erich, L.

    1981-06-01

    ESR studies have determined that ionizing radiation damage of hexobarbital (5-cyclohexenyl-1,5-dimethylbarbituric acid) causes the formation of a free radical (A) by hydrogen abstraction from the cyclohexenyl group. Hyperfine coupling tensors were determined for coupling of the unpaired electron to four protons. Visible light of wavelengths near 450 nm reversibly converts this radical to a second free radical (B) which also has the unpaired electron localized in the cyclohexenyl group. The activation energy for a thermally induced reverse conversion (B ..-->.. A) was determined to be 1.4 eV.

  8. The use of ionising radiation from 60CO gamma source in controlling mouldiness in dried cocoa beans

    International Nuclear Information System (INIS)

    Appiah, Victoria

    2001-01-01

    Mouldiness in stored cocoa beans in Ghana and the production of aflatoxin have been studied. Based on actual weight of discarded beans, mouldy beans have been estimated to constitute 0.13 % and 0.00002 % of marketable beans at the farmers' level and the buying agents' depots respectively in the Tafo District. This is contrasted with an estimated value of 0.16 % obtained in a questionnaire type study involving farmers. Estimated mouldy beans at the Tema port was 0.69 % per year (based on the cut test) representing a financial loss of $1,688,637.19 per year at $989/T should the mouldy beans be discarded. Fifty-eight (58) internally- and externally- borne fungal species were isolated from dried cocoa beans. Of these, forty-eight (48) were internally- borne and ten (10) were superficial. Twenty-nine (29) of the internally occurring fungi have been recorded for the first time on cocoa beans in Ghana. Twenty-six (26) of the fungi isolated belong to Aspergillus group. They included A. parasiticus and A. flavus, which can produce aflatoxins. Five (5) belong to Penicillium, eight (8) to Fusarium and nineteen (19) to other species. Ionising radiation effectively controlled fungi associated with mouldiness in cocoa beans in a dose - dependent manner. A radiation dose of 6 kGy completely inactivated the moulds. A. flavus and A. tamarii were the most radiation - resistant moulds encountered. The moisture content of the beans before, during and after irradiation influenced the effect of radiation. The relative humidity during storage and the type of packaging also influenced the radiation effect. Conidia of A. flavus subjected to moist heat at temperatures 20 0 C to 60 0 C for 2.5, 5 and 10 min respectively were not significantly affected by heating up to 50 0 C. Heating an aqueous conidial suspension at 60 0 C for at least 2.5 min reduced the number of fungal colonies by at least 5 log cycles when the suspension was assayed on agar plate media. Heating at 59 0 C for 10 min completely inactivated the wet conidia of A. flavus. When a combination of moist heat and radiation was applied to A. flavus, in different media, the inactivation dose was different in each medium. In vitro studies on A. flavus in suspension showed that the fungus could be inactivated by applying a combination of moist heat at 50 0 C for 10 min followed by 1.0 kGy radiation. A combination of moist heat at 80 0 C and radiation at 3.5 kGy produced the same effect with cocoa beans. Storage of beans for 28 days at 75% RH suppressed fungal growth while storage at 90% RH resulted in mouldiness of beans previously inoculated with A. flavus conidia and treated with heat and radiation. No aflatoxin was detected in cocoa beans after 4 weeks following inoculation with conidia of a toxigenic A. flavus strain and irradiation at 10 kGy. Without irradiation, inoculated beans became mouldy and produced aflatoxin B1. Irradiation at 10 kGy could not destroy the already formed aflatoxin. Radiation up to 6 kGy gave cocoa butter with free fatty acid, melting point, saponification value, and unsaponifiable matter levels comparable with factory standards. Exposure of beans to radiation did not have any effect on the rancidity of the extracted cocoa butter. Exposure of beans to radiation only or to radiation after heat treatment also did not affect the rate at which cocoa butter solidified. No significant differences in taste, colour and flavour were detected in cocoa drink prepared from cocoa powder produced from irradiated beans. (au)

  9. Characterization of a gamma-ray tracking array: A comparison of GRETINA and Gammasphere using a {sup 60}Co source

    Energy Technology Data Exchange (ETDEWEB)

    Lauritsen, T., E-mail: torben@anl.gov [Argonne National Laboratory, Argonne, IL60439 (United States); Korichi, A. [C.S.N.S.M., IN2P3-CNRS, bat 104-108, F-91405 Orsay Campus (France); Zhu, S. [Argonne National Laboratory, Argonne, IL60439 (United States); Wilson, A.N. [University of the West of Scotland, Paisley (United Kingdom); Weisshaar, D. [NSCL, Michigan State University (United States); Dudouet, J. [I.P.N. Lyon, IN2P3-CNRS, Lyon Campus (France); Ayangeakaa, A.D.; Carpenter, M.P. [Argonne National Laboratory, Argonne, IL60439 (United States); Campbell, C.M. [Lawrence Berkeley National Laboratory, Berkeley, CA94720 (United States); Clément, E. [GANIL, CEA/DSM-CNRS/IN2P3, BP 55027, F-14076 Caen (France); Crawford, H.L.; Cromaz, M.; Fallon, P. [Lawrence Berkeley National Laboratory, Berkeley, CA94720 (United States); Greene, J.P.; Janssens, R.V.F.; Khoo, T.L. [Argonne National Laboratory, Argonne, IL60439 (United States); Lalović, N. [Department of Physics, Lund University, SE-22100 Lund (Sweden); GSI Helmholtzzentrum für Schwerionenforschung GmbH, D-64291 Darmstadt (Germany); Lee, I.Y.; Macchiavelli, A.O [Lawrence Berkeley National Laboratory, Berkeley, CA94720 (United States); Perez-Vidal, R.M. [Instituto de Fisica Corpuscular, CSIC-Universitat de Valencia, E-46920 Valencia (Spain); and others

    2016-11-11

    In this paper, we provide a formalism for the characterization of tracking arrays with emphasis on the proper corrections required to extract their photopeak efficiencies and peak-to-total ratios. The methods are first applied to Gammasphere, a well characterized 4π array based on the principle of Compton suppression, and subsequently to GRETINA. The tracking efficiencies are then discussed and some guidelines as to what clustering angle to use in the tracking algorithm are presented. It was possible, using GEANT4 simulations, to scale the measured efficiencies up to the expected values for the full 4π implementation of GRETA.

  10. Real-Time Telemetric Monitoring in Whole-Body 60Co Gamma-Photon Irradiated Rhesus Macaques (Macaca mulatta)

    Science.gov (United States)

    2010-01-01

    clinically healthy adult male rhesus maca - ques (M. mulatta), 7–13 kg and 5–14 years of age, were obtained from the non-naı̈ve pool of NHPs of the US...11/9/2009. 15