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Sample records for qian keiji murakmi

  1. Sima Qian and His Historical Records

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    <正> Sima Qian (145-86 BC), a native of Xiayang (now Hancheng, Shaanxi Province), is regarded as China’s greatest historiographer. He is best known for his history book, Historical Records (Shi Ji).Son of Sima Tan, who held the post of the Grand Historian (Tai Shi Ling ) of the Han Dynasty (206 BC-AD 25), Sima

  2. Stamatina Dimakopoulou onMing-QianMa’s Poetry as Re-Reading

    Directory of Open Access Journals (Sweden)

    2010-05-01

    Full Text Available Ming-Qian Ma. Poetry as Re-Reading: American Avant-Garde Poetry and the Poetics of Counter-Method. Evanston, Illinois: Northwestern University Press, 2008. Pp. 301. ISBN 978-0810124851In the Preface to the 1979 Enlarging the Temple: New Directions in American Poetry during the 1960s – Charles Altieri formulated a re-alignment that defined much criticism of postwar and contemporary American poetry to come. Altieri set “the work of Heidegger and the later Wittgenstein,” in resonance with the “i...

  3. O CAMINHO DA NEGATIVIDADE ENTRE OCIDENTE E ORIENTE: A RECEPÇÃO CONTEMPORÂNEA DE MESTRE ECKHART EM KEIJI NISHITANI

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    Luiz Fernando Fontes-Teixeira

    2015-07-01

    Full Text Available O diálogo entre Ocidente e Oriente faz parte da mais recente história da filosofia contemporânea. Frente aos esforços de apresentar o Budismo japonês aos ocidentais, Teitarō Suzuki, chamado Daisetsu, comentou que, dentre os pensadores da filosofia ocidental, “Eckhart é aquele que está guiando o Zen para dentro do Ocidente”. O interesse da filosofia japonesa contemporânea por Mestre Eckhart, sobretudo dentre os autores da chamada Escola de Kyōto, representa não apenas uma mera aproximação por empatia, mas uma profunda introspecção da mística medieval como possibilidade de manifestar filosoficamente as bases do Zen Budismo, abrindo caminho para uma interculturalidade filosófica inédita no século XX. A despeito de sua presença constante (por vezes explícita, por vezes implícitas em diversos filósofos japoneses contemporâneos, foi Keiji Nishitani o pensador que com maior devoção refletiu sobre a obra de Eckhart. A partir desse diálogo, talvez seja possível investigar até que ponto uma recepção contemporânea de Mestre Eckhart possa porventura reverberar uma meditação pertinente aos problemas cotidianos do século XXI e este é, sem embargo, o intuito deste trabalho.

  4. Da-Bu-Yin-Wan and Qian-Zheng-San to Neuroprotect the Mouse Model of Parkinson’s Disease

    Directory of Open Access Journals (Sweden)

    Xiao-Gang Gong

    2014-01-01

    Full Text Available Da-Bu-Yin-Wan (DBYW and Qian-Zheng-San (QZS, two classic traditional Chinese medicinal formulas, were clinically employed to treat Parkinson’s disease (PD. Our previous studies demonstrated neuroprotective effects of them on mitochondrial function in PD mice induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP. The purpose of this research was to investigate their possible mechanisms in the light of mitochondrial ATP-sensitive potassium (mitoKATP channels. The neuroprotective effect of DBYW and QZS on dopamine (DA neurons in substantia nigra (SN in the MPTP-induced PD mice was investigated by behavioral test (pole test and immunohistochemistry. Adenosine triphosphate (ATP level in the midbrain tissue was detected by firefly luciferase method. MitoKATP channel subunits SUR1 and Kir6.2 mRNA and protein expressions were tested by real-time PCR (RT-PCR and Western blot. It was observed that DBYW and/or QZS served to ameliorate MPTP-induced behavioral impairment and prevent the loss of substantia nigra dopamine neurons, as well as increase ATP level in the midbrain tissue and downregulate SUR1 expression at mRNA and protein levels with no marked influence on Kir6.2. We concluded that DBYW and QZS exhibit neuroprotective effects probably through the regulation of ATP level and mitoKATP channel subunit expressions.

  5. Epigenetic battle of the sexes. Comment on: ;Epigenetic game theory: How to compute the epigenetic control of maternal-to-zygotic transition; by Qian Wang et al.

    Science.gov (United States)

    Wu, Song

    2017-03-01

    Qian Wang et al. present an interesting framework, named epigenetic game theory, for modeling sex-based epigenetic dynamics during embryogenesis from a new viewpoint of evolutionary game theory [1]. That is, epigenomes of sperms and oocytes may coordinate through either cooperation or competition, or both, to affect the fitness of embryos. The work uses a set of ordinary differential equations (ODEs) to describe longitudinal trajectories of DNA methylation levels in both parental and maternal gametes and their dependence on each other. The insights gained from this review, i.e. dynamic methylation profiles and their interaction are potentially important to many fields, such as biomedicine and agriculture.

  6. A mathematical view for ordinary differential equation models. Comment on ;Epigenetic game theory: How to compute the epigenetic control of maternal-to-zygotic transition; by Qian Wang et al.

    Science.gov (United States)

    Fu, Guifang

    2017-03-01

    Qian Wang et al. have written an interesting article to propose a modeling framework named epiGame in this issue of Physics of Life Reviews [1]. The epiGame framework models how the methylation state of paternal and maternal genomes regulates the embryogenesis as an ecological system in which two highly distinct and specialized gametes coordinate through either cooperation or competition, or both, to maximize the fitness of embryos. Qian Wang et al. also provide solid simulation studies and real data analysis to validate the correctness of their epiGame framework. The importance of embryo development and fertility mechanism cannot be overemphasized, hence, I think that the present review by Qian Wang et al. will stand as a useful modeling guide for practicing biologists or researchers in fertility health to quantify how sperms and oocytes interact through epigenetic process to determine embryo development. In addition, it will serve as a source of many important references to work in the reproductive biology field.

  7. Therapeutic effects of Qian-Yu decoction and its three extracts on carrageenan-induced chronic prostatitis/chronic pelvic pain syndrome in rats.

    Science.gov (United States)

    Zhang, Keda; Zeng, Xiaobin; Chen, Yonggang; Zhao, Rong; Wang, Hui; Wu, Jinhu

    2017-01-25

    Qian-Yu decoction (QYD) is a traditional Chinese medicinal recipe composed of Radix astragali (Astragalus membranaceus (Fisch.) Bunge var. mongholicus (Bunge) P.K. Hsiao, Fabaceae ), Herba epimedii (Epimedium brevicornum Maxim., Berberidaceae), Herba leonuri (Leonurus japonicus Houtt., Lamiaceae), Cortex phellodendri (Phellodendron chinense Schneid., Rutaceae) and Radix achyranthis bidentatae (Achyranthes bidentata Bl., Amaranthaceae). This study aimed to evaluate the therapeutic activity of QYD against carrageenan-induced chronic prostatic/chronic pelvic pain syndrome (CP/CPPS) in rats and further elucidate its effective components. Three types of components, total polysaccharides, total flavonoids and total saponins were separately extracted from QYD. Carrageenan-induced CP/CPPS rats were intragastrically administered with lyophilized product of QYD, individual extracts and all the combined forms of extracts for three weeks. Prostatic index (PI) was determined and histopathological analysis was performed. The levels of tumor necrosis factor alpha (TNF-α), interleukin-1 beta (IL-1β), cyclooxygenase-2 (COX-2) and prostaglandin E2 (PEG2) in rat prostate tissues were measured using ELISA. The production of inducible nitric oxide synthase (iNOS) was evaluated by an enzymatic activity assay, and the release of nitric oxide (NO) was determined by a nitrate/nitrite assay. Treatment with QYD significantly ameliorated the histological changes of CP/CPPS rats and reduced the PI by 44.3%, with a marked downregulation of TNF-α (42.8% reduction), IL-1β (45.3%), COX-2 (36.6%), PGE2 (44.2%), iNOS (54.1%) and NO (46.0%). Each of three extracts attenuated the symptom of CP/CPPS, but much more weakly than QYD. The combined administration of three extracts showed efficacy comparable to that of QYD while better than that of any combination of two extracts. A principal component analysis of the six inflammatory mediators as variables indicated that the effects of TS on CP

  8. 从钱乙五脏辨证论治抽动障碍理论探讨%Theoretical Discussion of Treating Tic Disorder with Five-Zang Syndrome Differentiation of QIAN Yi

    Institute of Scientific and Technical Information of China (English)

    贾慧; 钞建峰

    2017-01-01

    Five-zang syndrome differentiation of QIAN Yi has important value for the diagnosis and treat-ment of tic disorder in children. Tic disorder involves more than one zang organ ,and it usually affects five zang organs at the same time. Liver wind is the core of disease mechanism ,usually with lung deficiency at-tacked by external leading to internal wind,or with spleen deficiency leading to wood over inhibiting earth, or heart heat with wind fire,or with yin deficiency failing to nourish wood. The treatment should follow the principle of tonfiying and purging five zang organs,that extinguishing liver wind is the essential method,in combination with clearing and dispersing lung qi,or with tonifying earth and suppressing wood,or with clear-ing heart fire,or with nourishing yin and tonifying kidneys.%钱乙五脏辨证对儿童抽动障碍的辨治具有重要的价值.抽动障碍非一脏之病,而是"五脏同病",肝风为病机之核心,或兼肺虚受邪、外风引动内风,或兼脾虚土衰、木乘脾土,或兼心热火旺、风火相搏,或兼肾阴亏虚、水不涵木.治疗尊五脏补泻的原则,以调肝熄风为本,针对不同的病机,或清宣肺气,或扶土抑木,或清心泻火,或养阴补肾.

  9. Qian Yang Yu Yin Granule-containing serum inhibits angiotensin II-induced proliferation, reactive oxygen species production, and inflammation in human mesangial cells via an NADPH oxidase 4-dependent pathway.

    Science.gov (United States)

    Ding, Kang; Wang, Yan; Jiang, Weimin; Zhang, Yu; Yin, Hongping; Fang, Zhuyuan

    2015-03-25

    Qian Yang Yu Yin Granule (QYYYG), a traditional Chinese herbal medicine, has been indicated for renal damage in hypertension for decades in China, but little remains known regarding its underlying molecular mechanism. Therefore, we performed the current study in order to investigate the underlying molecular mechanism of QYYYG in the treatment of hypertensive renal damage. We hypothesize that QYYYG relieves hypertensive renal injury through an angiotensin II (Ang II)-nicotinamide adenine dinucleotide phosphate (NAPDH)-oxidase (NOX)-reactive oxygen species (ROS) pathway. In this study, we investigated the effects of QYYYG-containing serum (QYGS) in human mesangial cells (HMCs) against Ang II-induced cell proliferation, ROS production, and inflammation through the seropharmacological method. We found that QYGS could inhibit cell proliferation in Ang II-treated HMCs. In addition, QYGS considerably suppressed production of ROS, decreased mRNA and protein expression of NAPDH-oxidase 4 (NOX4), p22 (phox) , and activated Ras-related C3 botulinum toxin substrate 1 (GTP-Rac1); as well as counteracted the up-regulation of inflammatory markers including tumor necrosis factor-α (TNF-α), nuclear factor-κB (NF-κB) p65, and interleukin 6 (IL-6). These effects were further confirmed in HMCs transfected with specific small interfering RNA (siRNA) targeting NOX4. Taken together, these results suggest that a NOX4-dependent pathway plays an important role in regulating the inhibitory effect of QYGS. Our findings provide new insights into the molecular mechanisms of QYYYG and their role in the treatment of hypertensive nephropathy.

  10. The analysis of Stability reliability of Qian Tang River seawall

    Science.gov (United States)

    Wu, Xue-Xiong

    2017-11-01

    Qiantang River seawall due to high water soaking pond by foreshore scour, encountered during the low tide prone slope overall instability. Considering the seawall beach scour in front of random change, using the simplified Bishop method, combined with the variability of soil mechanics parameters, calculation and analysis of Qiantang River Xiasha seawall segments of the overall stability.

  11. Response to Ridgeway, Dunston, and Qian: On Methodological Rigor: Has Rigor Mortis Set In?

    Science.gov (United States)

    Baldwin, R. Scott; Vaughn, Sharon

    1993-01-01

    Responds to an article in the same issue of the journal presenting a meta-analysis of reading research. Expresses concern that the authors' conclusions will promote a slavish adherence to a methodology and a rigidity of thought that reading researchers can ill afford. (RS)

  12. Millal riigid finantskriisidest välja kasvavad? / Carmen M. Reinhart, Rong Qian, Kenneth Rogoff

    Index Scriptorium Estoniae

    Reinhart, Carmen M.

    2010-01-01

    Artiklis kirjutatakse pangandus-, välisvõla- ja inflatsioonikriiside esinemissagedusest ja kestvusest ning jõutakse järeldusele, et arenenud riikide panganduskriisidest väljakasvamisest pole praegu mingit põhjust rääkida. Skeem

  13. Qian et al., Afr J Tradit Complement Altern Med. (2016) 13(4):145 ...

    African Journals Online (AJOL)

    SD was a double of normal dose (28 ml/kg • d) and the low concentration SD constituted half of ... the animals were anesthetized with 1% chloral hydrate, and blood was drawn sterilely from the ... verapamil,which is an inhibitor of ABCG-2.

  14. Qian et al., Afr J Tradit Complement Altern Med. (2015) 12(6):77-89 ...

    African Journals Online (AJOL)

    Proff.Adewunmi

    As can be see in Figure 3, compared with the normal saline group (control group) at corresponding time point, four demolition party serum treatments inhibited cell .... 1.95±1.50*#. Note: * p < 0.05 compared with corresponding cell cycle phase in control group; # p < 0.05 in same drug serum group, cell cycle phases in 48 h.

  15. Evaluation of the antioxidant and endothelial protective effects of Lysimachia christinae Hance (Jin Qian Cao) extract fractions.

    Science.gov (United States)

    Wu, Ning-Hua; Ke, Zhi-Qiang; Wu, Shan; Yang, Xiao-Song; Chen, Qing-Jie; Huang, Sheng-Tang; Liu, Chao

    2018-04-10

    Lysimachia christinae Hance is a traditional Chinese medicine with diuretic, detumescent, and detoxifying effects. Our aimed to optimize the extraction protocol to maximize the yield of flavonoids from Lysimachia christinae Hance, and evaluate the pharmacological activities of four fractions, namely, petroleum ether (PE), ethyl acetate (EA), n-butanol (NB), and aqueous (AQ) fractions, of the ethanolic extract of Lysimachia christinae Hance. The flavonoid monomers in the crude extract were characterized via high performance liquid chromatography (HPLC), were used as markers for extract quality control and standardization. The total flavonoid, total phenolic, and total polysaccharide contents of each fraction were determined by spectrophotometry. Further, the in vitro free radical (diphenylpicrylhydrazyl, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid), superoxide, and hydroxyl radicals) scavenging activities, and antioxidant capacity in endothelial cells were evaluated for each fraction. After optimizing the extraction protocol to maximize the total flavonoid yield from L. christinae Hance, the NB fractions had the highest total flavonoid (39.4 ± 4.55 mg RE/g), total phenolic (41.1 ± 3.07 mg GAE/g) and total polysaccharide (168.1 ± 7.07 mg GE/g); In addition, the NB fraction of the ethanolic extract of L. christinae Hance reveal the strongest radical-scavenging activity, antioxidant activity and protective effects against H 2 O 2 -induced injury in HUVECs. Among the four fractions of L. christinae Hance, the NB fraction showed the most potent antioxidant and endothelial protective effects, which may be attributed to its high flavonoid, phenolic contents and optimal portfolio of different active ingredients of NB fractions of the ethanolic extract of L. christinae Hance. This study might improve our understanding of the pharmacological activities of L. christinae Hance, thereby facilitating its use in disease prevention and treatment.

  16. Measurement of cooling coil film heat transfer coefficient with polymer reaction proceeding in a stirred batch reactor; Jugo sonai ni okeru hanno shinko ni tomonau reikyaku coil no kyomaku netsudentatsu keisu no keiji henka

    Energy Technology Data Exchange (ETDEWEB)

    Yamamoto, K [Soken Chemical and Engineering Co. Ltd., Saitama (Japan); Nishi, K; Kaminoyama, M; Kamiwano, M [Yokohama National University, Yokohama (Japan). Faculty of Engineering

    1996-09-10

    In radical additional solution polymerization, the viscosity increases with reaction progress. It is important to evaluate beforehand the cooling capacity of the reactor, which worsens with the process. In this study, a stirred batch reactor with both a paddle and a helical screw impeller were studied, and measurements were made for the dynamic changes of the film heat transfer coefficient of the cooling coil with progress of the polymer reaction. We found the change could be evaluated by the calculating heat balance of the generated heat, the viscous dissipation energy and the sensible heat change under conditions of monomer conversion and changing viscosity. 11 refs., 7 figs.

  17. Investigation of spectral distribution and variation of irradiance with the passage time of CSI lamps which constitute a solar simulator; Solar simulator ni shiyosuru CSI lamp no supekutoru bunpu, hosha shodo no keiji henka ni kansuru chosa

    Energy Technology Data Exchange (ETDEWEB)

    Sugiyama, T; Yamada, T; Noguchi, T [Japan Quality Assurance Organization, Tokyo (Japan)

    1997-11-25

    Study was made on time-variation of the performance of CSI lamps for solar simulators. In order to accurately evaluate the standard heat collection performance of solar systems in a room, MITI installed an artificial solar light source in the Solar Techno-Center of Japan Quality Assurance Organization for trial use and evaluation. CSI lamp is superior in durability, and can simulate the solar light in the daytime. The light source is composed of 72 metal halide lamps of 1kW arranged in a plane of 3.5times3.5m. The study result on time-variation of a spectral distribution and irradiance by intermittent switching of lamps showed a sufficient durability of 2000h. To ensure the accuracy of a solar heat collector measurement system enough, periodic calibration is being carried out using reference goods. To ensure the reliability and stability for a switching system, periodic maintenance of a power source, stabilizer and electric system is also being carried out in addition to CSI lamps. The stable irradiance and accuracy are being kept by such maintenance and periodic exchange of lamps. 6 figs., 4 tabs.

  18. Game theory in epigenetic reprogramming. Comment on: ;Epigenetic game theory: How to compute the epigenetic control of maternal-to-zygotic transition; by Qian Wang et al.

    Science.gov (United States)

    Hsu, Fei-Man; Chen, Pao-Yang

    2017-03-01

    Von Neumann and Morgenstern published the Theory of Games and Economic Behavior in 1944, describing game theory as a model in which intelligent rational decision-makers manage to find their best strategies in conflict, cooperative or other mutualistic relationships to acquire the greatest benefit [1]. This model was subsequently incorporated in ecology to simulate the ;fitness; of a species during natural selection, designated evolutionary game theory (EGT) [2]. Wang et al. proposed ;epiGame;, taking paternal and maternal genomes as ;intelligent; players that compete, cooperate or both during embryogenesis to maximize the fitness of the embryo [3]. They further extended game theory to an individual or single cell environment. During early zygote development, DNA methylation is reprogrammed such that the paternal genome is demethylated before the maternal genome. After the reset, the blastocyst is re-methylated during embryogenesis. At that time, the paternal and maternal genomes have a conflict of interest related to the expression of their own genes. The proposed epiGame models such interactive regulation between the parental genomes to reach a balance for embryo development (equation (2)).

  19. Racism of prohibition and destruction of novels in QianLong dynasty%论乾隆朝小说禁毁的种族主义倾向

    Institute of Scientific and Technical Information of China (English)

    胡海义; 程国赋

    2006-01-01

    乾隆朝禁毁小说颇具特色.乾隆从狭隘的民族主义立场出发,为了维护满族旧习,以免其被汉族文化习俗所同化,专注于禁毁政治违碍小说,对淫秽小说却并不关注,企图以此泯灭汉人的民族意识,把汉族文化与满族旧习隔离,表现出鲜明的种族主义倾向.

  20. A new concept: Epigenetic game theory. Comment on: ;Epigenetic game theory: How to compute the epigenetic control of maternal-to-zygotic transition; by Qian Wang et al.

    Science.gov (United States)

    Zheng, Xiu-Deng; Tao, Yi

    2017-03-01

    The evolutionary significance of the interaction between paternal and maternal genomes in fertilized zygotes is a very interesting and challenging question. Wang et al. developed the concept of epigenetic game theory, and they try to use this concept to explain the interaction between paternal and maternal genomes in fertilized zygotes [1]. They emphasize that the embryogenesis can be considered as an ecological system in which two highly distinct and specialized gametes coordinate through either cooperation or competition, or both, to maximize the fitness of embryos under Darwinian selection. More specifically, they integrate game theory to model the pattern of coordination of paternal genome and maternal genomes mediated by DNA methylation dynamics, and they called this epigenetic game theory.

  1. Reaction mechanism of coal liquefaction: hydrogenolysis of model compound using synthetic pyrite as catalysts. 7. Property change of synthetic pyrite catalyst with the time after production; Sekitan ekika hanno kiko (model kagobutsu no hanno). 7. Gosei ryukatetsu shokubai no keiji henka ni tsuite

    Energy Technology Data Exchange (ETDEWEB)

    Ito, H.; Meno, H.; Uemaki, O.; Shibata, T.; Tsuji, T. [Hokkaido University, Sapporo (Japan)

    1996-10-28

    Reactions of various model compounds were investigated using synthetic pyrites for coal liquefaction. In this study, successive changes of the catalysts were investigated from the reactions of model compounds by using three different synthetic pyrites with the lapse of time after production. Benzyl phenyl ether, dibenzyl, and n-octylbenzene were used as model compounds. Reactions were conducted in an autoclave, into which sample, catalyst, decalin as solvent, and initial hydrogen pressure 10 MPa were charged. The autoclave was held at 450 or 475{degree}C of reaction temperature for 1 hour. The catalyst with a shorter lapse of time after production acted to hydrogen transfer, and inhibited the formation of condensation products due to the stabilization of decomposed fragment. It also acted to isomerization of materials by cutting alkyl side chains. When adding sulfur to the catalyst with longer lapse of time after production under these reaction conditions, it inhibited the formation of condensation products for the reaction of benzyl phenyl ether. However, it did not provide the effect for the reaction of n-octylbenzene. 5 refs., 3 figs.

  2. Numerical modeling of historical change of volcanic heat sources: Numerical modeling of heat and mass transport up to 1000 degree C; Kazansei netsugen no keiji henka no shumyureshon kaiseki: 1000 degree C madeno netsu{center{underscore}dot}ryutai shumyureshon kaiseki

    Energy Technology Data Exchange (ETDEWEB)

    Hanano, Mineyuki [JMC Geothermal Engineering Corp., Iwate (Japan)

    1998-12-01

    Temperature structure and its historical change around volcanos has been of interest for volcanology, geothermal development, etc. Magmatic intrusives have temperatures ranging from 700 to 850 degree C. Thus, there exists super-critical fluid around them. Numerical modeling of temperature changes around young volcanos and their heat sources thus requires treatment of the super-critical fluid. We describe one method for effective treatment of the super-critical fluid in the numerical modeling of porous media for the purpose of solving large-scale high-temperature problems of such phenomena. (author)

  3. Integrating evolutionary game theory into epigenetic study of embryonic development. Comment on ;Epigenetic game theory: How to compute the epigenetic control of maternal-to-zygotic transition; by Qian Wang et al.

    Science.gov (United States)

    Wang, Zuoheng

    2017-03-01

    DNA methylation is an essential component in the epigenetic regulation of embryonic development, and plays a crucial role in various biological processes, including repression of gene transcription, parent-of-origin genomic imprinting, and X-chromosome inactivation [1-5]. Understanding the epigenetic processes in different stages of embryo development has become an important research topic in the field. It has potential to offer new insight into reproductive medicine and contribute to the improvement of long-term health outcomes.

  4. Using game theory to investigate the epigenetic control mechanisms of embryo development: Comment on: "Epigenetic game theory: How to compute the epigenetic control of maternal-to-zygotic transition" by Qian Wang et al.

    Science.gov (United States)

    Zhang, Le; Zhang, Shaoxiang

    2017-03-01

    A body of research [1-7] has already shown that epigenetic reprogramming plays a critical role in maintaining the normal development of embryos. However, the mechanistic quantitation of the epigenetic interactions between sperms and oocytes and the related impact on embryo development are still not clear [6,7]. In this study, Wang et al., [8] develop a modeling framework that addresses this question by integrating game theory and the latest discoveries of the epigenetic control of embryo development. Copyright © 2017 Elsevier B.V. All rights reserved.

  5. Applications and extensions of epigenetic game theory. Comment on: ;Epigenetic game theory: How to compute the epigenetic control of maternal-to-zygotic transition; by Qian Wang et al.

    Science.gov (United States)

    Wang, Yaqun

    2017-03-01

    The authors are to be congratulated for a thought-provoking article [1], which reviews the epigenetic game theory (epiGame) that utilizes differential equations to study the epigenetic control of embryo development. It is a novel application of evolutionary game theory and provides biology researchers with useful methodologies to address scientific questions related to biological coordination of competition and cooperation.

  6. Epigenetic information in gametes: Gaming from before fertilization. Comment on ;Epigenetic game theory: How to compute the epigenetic control of maternal-to-zygotic transition; by Qian Wang et al.

    Science.gov (United States)

    Shi, Junchao; Zhang, Xudong; Liu, Ying; Chen, Qi

    2017-03-01

    In their interesting article [1] Wang et al. proposed a mathematical model based on evolutionary game theory [2] to tackle the fundamental question in embryo development, that how sperm and egg interact with each other, through epigenetic processes, to form a zygote and direct successful embryo development. This work is based on the premise that epigenetic reprogramming (referring to the erasure and reconstruction of epigenetic marks, such as DNA methylation and histone modifications) after fertilization might be of paramount importance to maintain the normal development of embryos, a premise we fully agree, given the compelling experimental evidence reported [3]. Wang et al. have specifically chosen to employ the well-studied DNA methylation reprogramming process during mammalian early embryo development, as a basis to develop their mathematical model, namely epigenetic game theory (epiGame). They concluded that the DNA methylation pattern in mammalian early embryo could be formulated and quantified, and their model can be further used to quantify the interactions, such as competition and/or cooperation of expressed genes that maximize the fitness of embryos. The efforts by Wang et al. in quantitatively and systematically analyzing the beginning of life apparently hold value and represent a novel direction for future embryo development research from both theoretical and experimental biologists. On the other hand, we see their theory still at its infancy, because there are plenty more parameters to consider and there are spaces for debates, such as the cases of haploid embryo development [4]. Here, we briefly comment on the dynamic process of epigenetic reprogramming that goes beyond DNA methylation, a dynamic interplay that involves histone modifications, non-coding RNAs, transposable elements et al., as well as the potential input of the various types of 'hereditary' epigenetic information in the gametes - a game that has started before the fertilization.

  7. Epigenetic game theory and its application in plants. Comment on: ;Epigenetic game theory: How to compute the epigenetic control of maternal-to-zygotic transition; by Qian Wang et al.

    Science.gov (United States)

    Zhang, Yuan-Ming; Zhang, Yinghao; Guo, Mingyue

    2017-03-01

    Wang's et al. article [1] is the first to integrate game theory (especially evolutionary game theory) with epigenetic modification of zygotic genomes. They described and assessed a modeling framework based on evolutionary game theory to quantify, how sperms and oocytes interact through epigenetic processes, to determine embryo development. They also studied the internal mechanisms for normal embryo development: 1) evolutionary interactions between DNA methylation of the paternal and maternal genomes, and 2) the application of game theory to formulate and quantify how different genes compete or cooperate to regulate embryogenesis through methylation. Although it is not very comprehensive and profound regarding game theory modeling, this article bridges the gap between evolutionary game theory and the epigenetic control of embryo development by powerful ordinary differential equations (ODEs). The epiGame framework includes four aspects: 1) characterizing how epigenetic game theory works by the strategy matrix, in which the pattern and relative magnitude of the methylation effects on embryogenesis, are described by the cooperation and competition mechanisms, 2) quantifying the game that the direction and degree of P-M interactions over embryo development can be explained by the sign and magnitude of interaction parameters in model (2), 3) modeling epigenetic interactions within the morula, especially for two coupled nonlinear ODEs, with explicit functions in model (4), which provide a good fit to the observed data for the two sexes (adjusted R2 = 0.956), and 4) revealing multifactorial interactions in embryogenesis from the coupled ODEs in model (2) to triplet ODEs in model (6). Clearly, this article extends game theory from evolutionary game theory to epigenetic game theory.

  8. Two new species of genus Rhopalopsole (Insecta, Plecoptera, Leuctridae from China

    Directory of Open Access Journals (Sweden)

    Qian Yuhan

    2011-12-01

    Full Text Available Two new species of Rhopalopsole Klapálek from China are described: R. exiguspina Du & Qian, sp. n. and R. ampulla Du & Qian, sp. n., which were collected in Guizhou province, China.

  9. Two new species of genus Rhopalopsole (Insecta, Plecoptera, Leuctridae) from China

    Science.gov (United States)

    Yu-Han, Qian; Yu-Zhou, Du

    2011-01-01

    Abstract Two new species of Rhopalopsole Klapálek from China are described: Rhopalopsole exiguspina Du & Qian, sp. n. and Rhopalopsole ampulla Du & Qian, sp. n., which were collected in Guizhou province, China. PMID:22287913

  10. Study on the Pauropoda from Tibet, China. Part I. The genera Decapauropus and Hemipauropus (Myriapoda

    Directory of Open Access Journals (Sweden)

    Chang-Yuan Qian

    2018-04-01

    Full Text Available Three new species of family Pauropodidae: Decapauropus biconjugarus Qian & Bu, sp. n., D. tibeticus Qian & Bu, sp. n. and Hemipauropus quadrangulus Qian & Bu, sp. n. are described and illustrated from southeastern Tibet, China. The genus Hemipauropus is recorded for the first time from China. This is the second report of pauropods from Tibet.

  11. Investigating the origin of the Chinese name for alfalfa

    Science.gov (United States)

    Sun, Q. Z.; Xu, L. J.; Tang, X. J.; Ma, J. T.; Wang, D.; Li, D.; Liu, Q.; Tao, Y.; Li, F.

    2017-02-01

    It is assured that alfalfa (Medicago sativa L.) was introduced in Han dynasty. There are cognitive differences on whether Zhang Qian introduced alfalfa. Based on the previous studies, research inductive method was used. The relationship between Zhang Qian and alfalfa introduction was analyzed from the motivation, experience and influence of Zhang Qian to the Western Regions and the image generation of Zhang Qian brought back alfalfa’s seeds. Till to now, there are four opinions about Zhang Qian introducing Alfalfa seeds, including : (1) Zhang Qian introduced alfalfa seeds;(2) Zhang Qian did not introduce alfalfa seeds;(3) the information of Zhang Qian transferring alfalfa;(4)for commemoration Zhang Qian to the Western Regions. Although there are not direct historical materials to support Zhang Qian brought alfalfa seed to Han dynasty, it believes and confirms that the introducing of alfalfa is inextricably interwoven with Zhang Qian’s western travel. Zhangqian brought relative information from western regions during the introduction, which was the basis of non-native theory, and after that, Chinese began to plant alfalfa in Han dynasty., According to historical literatures, it is clear that the Chinese diplomat brought alfalfa seeds back to China. Alfalfa, as the favorite forage to Ferghana horse, have been already planted in Dawan in Han dynasty. Despite the debate, Zhangqian played an important pioneering role in introducing alfalfa.

  12. Integrable System and $N=2$ Supersymmetric Yang-Mills Theory

    OpenAIRE

    Nakatsu, T.; Takasaki, K.

    1996-01-01

    Comment: 6 pages,latex file with sprocl.sty, no figures, to appear in the Proceedings of the Workshop (Talk presented at the Workshop "Frontiers in Quantum Field Theory" in honor of the 60th birthday of Prof. Keiji Kikkawa, Osaka, Japan, December 1995

  13. The U.S.-Japan Security Relationship After the Cold War

    Science.gov (United States)

    1993-01-01

    Section, Overseas Research Department of JETRO 2. Hideya Kurata, research fellow, Japan Institute of International Affairs 3. Keiji Kobayashi , senior...pp. 184-193. Nishimura, Shigeki, "Senryaku tenkan no sessoku o haisu," Voice, September 1992, pp. 94-105. Biblography 103 Nobuaki , Takahashi

  14. Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors, supplement to: Dineshram, R; Chandramouli, K; Ko, W K Ginger; Zhang, Huoming; Qian, Pei Yuan; Ravasi, Timothy; Thiyagarajan, Vengatesen (2016): Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors. Global Change Biology, 22(6), 2054-2068

    KAUST Repository

    Dineshram, R; Chandramouli, Kondethimmanahalli; Ko, W K Ginger; Zhang, Huoming; Qian, Pei Yuan; Ravasi, Timothy; Thiyagarajan, Vengatesen

    2016-01-01

    survival bottleneck, which will be pressured by different anthropogenically induced climate change-related variables. Therefore, it is important to understand how metamorphosing larvae interact with emerging climate change stressors. To predict how larvae

  15. Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors, supplement to: Dineshram, R; Chandramouli, K; Ko, W K Ginger; Zhang, Huoming; Qian, Pei Yuan; Ravasi, Timothy; Thiyagarajan, Vengatesen (2016): Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors. Global Change Biology, 22(6), 2054-2068

    KAUST Repository

    Dineshram, R

    2016-01-01

    The metamorphosis of planktonic larvae of the Pacific oyster (Crassostrea gigas) underpins their complex life-history strategy by switching on the molecular machinery required for sessile life and building calcite shells. Metamorphosis becomes a survival bottleneck, which will be pressured by different anthropogenically induced climate change-related variables. Therefore, it is important to understand how metamorphosing larvae interact with emerging climate change stressors. To predict how larvae might be affected in a future ocean, we examined changes in the proteome of metamorphosing larvae under multiple stressors: decreased pH (pH 7.4), increased temperature (30 °C), and reduced salinity (15 psu). Quantitative protein expression profiling using iTRAQ-LC-MS/MS identified more than 1300 proteins. Decreased pH had a negative effect on metamorphosis by down-regulating several proteins involved in energy production, metabolism, and protein synthesis. However, warming switched on these down-regulated pathways at pH 7.4. Under multiple stressors, cell signaling, energy production, growth, and developmental pathways were up-regulated, although metamorphosis was still reduced. Despite the lack of lethal effects, significant physiological responses to both individual and interacting climate change related stressors were observed at proteome level. The metamorphosing larvae of the C. gigas population in the Yellow Sea appear to have adequate phenotypic plasticity at the proteome level to survive in future coastal oceans, but with developmental and physiological costs.

  16. Two new species of Rhopalopsole (Plecoptera: Leuctridae) from China.

    Science.gov (United States)

    Qian, Yu-Han; DU, Yu-Zhou

    2017-06-02

    Two new species of the genus Rhopalopsole, R. brevidigitata Qian & Du, sp. nov. and R. faciursina Qian & Du, sp. nov. are described and illustrated from Yunnan Province of southwestern China. The new species are compared to similar taxa in the R. vietnamica and R. shaanxiensis groups, respectively.

  17. Improvement of Binary Analysis Components in Automated Malware Analysis Framework

    Science.gov (United States)

    2017-02-21

    AFRL-AFOSR-JP-TR-2017-0018 Improvement of Binary Analysis Components in Automated Malware Analysis Framework Keiji Takeda KEIO UNIVERSITY Final...TYPE Final 3. DATES COVERED (From - To) 26 May 2015 to 25 Nov 2016 4. TITLE AND SUBTITLE Improvement of Binary Analysis Components in Automated Malware ...analyze malicious software ( malware ) with minimum human interaction. The system autonomously analyze malware samples by analyzing malware binary program

  18. Investigation of the Antiasthmatic Properties of Ethanol Extract of ...

    African Journals Online (AJOL)

    Investigation of the Antiasthmatic Properties of Ethanol Extract of Callophyllis japonica in Mice. WS Park, KS Lee, JH Chun, SH Urm, DS Lee, DY Lee, SG Park, SK Seo, SJ Heo, ZJ Qian, WK Jung, IW Choi ...

  19. Thirty-seven transcription factor genes differentially respond to a ...

    Indian Academy of Sciences (India)

    Plant transcription factors and insect defence si. Thirty-seven transcription factor genes differentially respond to a harpin protein and affect resistance to the green peach aphid in Arabidopsis. HUNLIN. PIN. RUOXUE LIŲ, BEIBEI LÜ, XIAOMENG WANG, CHUNLING ZHANG, SHUPING ZHANG, JUN QIAN, LEI CHEN,.

  20. Journal of Chemical Sciences | Indian Academy of Sciences

    Indian Academy of Sciences (India)

    Home; Journals; Journal of Chemical Sciences; Volume 128; Issue 6. Triflic Anhydride-Mediated Beckmann Rearrangement Reaction of Β-Oximyl Amides: Access to 5-Iminooxazolines. MANGFEI YU QIAN ZHANG JIA WANG PENG HUANG PENGFEI YAN RUI ZHANG DEWEN DONG. Regular Article Volume 128 Issue 6 ...

  1. Dangjia Village: Holding Its Ground

    Institute of Scientific and Technical Information of China (English)

    LUZHONGMIN

    2005-01-01

    THE Yellow River, the second longest in China, billows through the Shanxi-Shaanxi canyons. On its west bank in Shaanxi is an historical city - Hancheng, birthplace of Sima Qian, author of the Records of the Historian and inaugurator of Chinese biography. Within the city limits is a village with a 670-year history - Dangjia.

  2. Tropical Journal of Pharmaceutical Research - Vol 15, No 3 (2016)

    African Journals Online (AJOL)

    Wan Wu, Wei Chen, Qiong Jin, 447-451 ... Chung-Shih Chen, Mei-Lin Wang, Rosa Huang Liu, Shih-Pin Chen, Tsong-Ming Lu, Wei-Yu Tsai, Chien-Fu Huang, ... Yan-Qiong Li, Rui Hu, Li-Hui Zhong, Qian Sun, You-Ping Yan, 527-533.

  3. Assessing the Depth and Breadth of Vocabulary Knowledge with Listening Comprehension

    Science.gov (United States)

    Teng, Feng

    2014-01-01

    This study was inspired by Qian (1999) and Staehr (2009) and researched 88 Chinese learners who had already passed the College English Test 4 (CET). These learners volunteered to participate in the study regarding the depth and breadth of vocabulary knowledge and its relationship with listening comprehension, which was assessed by analyzing the…

  4. Journal of Chemical Sciences | Indian Academy of Sciences

    Indian Academy of Sciences (India)

    Home; Journals; Journal of Chemical Sciences; Volume 129; Issue 8. Three coordination compounds based on benzene tetracarboxylate ligand: syntheses, structures, thermal behaviors and luminescence properties. YUNLONG WU CHANGKUN XIA JUN QIAN JIMIN XIE. REGULAR ARTICLE Volume 129 Issue 8 August ...

  5. Pramana – Journal of Physics | Indian Academy of Sciences

    Indian Academy of Sciences (India)

    dimensional coupled double-distribution-function lattice Boltzmann models for compressible Navier–Stokes equations. RUO-FAN QIU YAN-CHENG YOU CHENG-XIANG ZHU RONG-QIAN CHEN JIAN-FENG ZHU. Research Article Volume 89 Issue ...

  6. Journal of Chemical Sciences | Indian Academy of Sciences

    Indian Academy of Sciences (India)

    Home; Journals; Journal of Chemical Sciences. Hai-Liang Zhu. Articles written in Journal of Chemical Sciences. Volume 121 Issue 4 July 2009 pp 463-470. Synthesis, antimicrobial activity of lamotrigine and its ammonium derivatives · Yong Qian Peng-Cheng Lv Lei Shi Rui-Qin Fang Zhong-Cheng Song Hai-Liang Zhu.

  7. Journal of Chemical Sciences | Indian Academy of Sciences

    Indian Academy of Sciences (India)

    CHEN AI-HUA1 MENG SU-CI2 ZHOU KAI1 WANG CONG-CONG1 ZHAO WEI2 WANG AI-JIAN2 QIAN JUN2. School of Chemistry & Chemical Engineering, Yancheng Institute of Technology, Yancheng 224051, People's Republic of China; School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang ...

  8. African Journal of Biotechnology - Vol 10, No 37 (2011)

    African Journals Online (AJOL)

    7130 ... G Lu, Y Shen, W Qian, L Shen, Q Qiao, M Li, Z Wang, 7136-7142 ... LN Zhen, K Wang, YJ Zhang, H Zhou, 7161-7165 ... Effect of cadmium hyperaccumulation on antioxidative defense and proline accumulation of Solanum nigrumL.

  9. Amplified fragment length polymorphism (AFLP) and genealogy ...

    African Journals Online (AJOL)

    STORAGESEVER

    2010-06-07

    Jun 7, 2010 ... Pang CY, Du XM, Ma ZY (2006). Evaluation of the introgressed lines and screening for elite germplasm in Gossypium, Chin. Sci. Bull. 51(1):. 304-312. Pieter V, Rene H, Marjo B (1995). AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res. 23(21): 4407-4414. Qian SY, Huang JQ, Zhou BL, Peng ...

  10. Does Mandarin spatial metaphor for time influence Chinese deaf signers’ spatio-temporal reasoning?

    NARCIS (Netherlands)

    Gu, Yan; Zheng, Yeqiu; Swerts, Marc; Gunzelmann, G.; Howes, A.; Tenbrink, T.; Davelaar, E. J.

    2017-01-01

    In Mandarin Chinese, the space-time word “前/qian” is used to express both the spatial concept of front/forward and the temporal concept of early/before (e.g., “前天/qian-tian”, literally front day, meaning the day before yesterday). This is consistent with the fact that Mandarin speakers can gesture

  11. Journal of Biosciences | Indian Academy of Sciences

    Indian Academy of Sciences (India)

    Home; Journals; Journal of Biosciences; Volume 31; Issue 5. Combinative effects of a bacterial type-III effector and a biocontrol bacterium on rice growth and disease resistance. Haiying Ren Ganyu Gu Juying Long Qian Yin Tingquan Wu Tao Song Shujian Zhang Zhiyi Chen Hansong Dong. Reviews Volume 31 Issue 5 ...

  12. Isolation and characterization of sixty sequences of cot-1 DNA from ...

    African Journals Online (AJOL)

    user

    2012-11-06

    Nov 6, 2012 ... this study were from U. t. ussuricus animals raised in the Beijing. Zoo. ..... Collins F, Guyer MS, Peterson J, Felsenfeld A, Wetterstrand KA,. Patrinos A ... B, Ni P, Lin R, Qian W, Wang G, Yu C, Nie W, Wang J, Wu Z, Liang.

  13. Research

    African Journals Online (AJOL)

    abp

    16 oct. 2017 ... Hong Jiang, Xu Qian, Guillermo Carroli and Paul Garner. Selective versus routine use of episiotomy for vaginal birth. Cochrane Database Syst Rev. 2017 February 8; 2: CD000081. Google Scholar. 17. Louis Kuhne. L'origine de la méthode Bain Detox in Bain de · désintoxication. Consulté le 15 avril 2017.

  14. http://dx.doi.org/10.4314/ajtcam.v10i4.6

    African Journals Online (AJOL)

    AJTCAM

    http://dx.doi.org/10.4314/ajtcam.v10i4.6. 36. CHEMOTHERAPEUTIC ACTIVITIES OF CARTHAMI FLOS AND ITS REVERSAL EFFECT ON. MULTIDRUG RESISTANCE IN CANCER CELLS. Jimmy Yiu-Cheong Wu*a, Zhi-Ling Yub, Wang-Fun Fongb, Yi-Qian Shia. aZhejiang Chinese Medical University, Hangzhou 310053, ...

  15. Journal of Biosciences | Indian Academy of Sciences

    Indian Academy of Sciences (India)

    Author Affiliations. Ruoxue Liu1 Beibei Lü1 Xiaomeng Wang1 Chunling Zhang1 Shuping Zhang1 Jun Qian1 Lei Chen1 Haojie Shi1 Hansong Dong1. State Ministry of Agriculture Key Laboratory of Monitoring and Management of Crop Pathogens and Insect Pests, Nanjing Agricultural University, Nanjing 210095, China ...

  16. Optimization of wind speed on dispersion of pollutants using ...

    Indian Academy of Sciences (India)

    Department of Chemical Engineering, National Institute of Technology Calicut, ... The classical approaches of dispersion model (Qian & Venkatram 2001; ..... of ambient fine organic carbon particles and source apportionment of PM2.5 in Indian ... matrix factorization and chemical mass balance applied to receptor modeling.

  17. Current treatment options for the management of patent ductus arteriosus

    Directory of Open Access Journals (Sweden)

    Takeuchi K

    2013-03-01

    Full Text Available Koh Takeuchi,1 Atsushi Hirota,2 Sachito Minegishi,1 Jotaro Kobayashi,1 Keiji Tsuchiya3 1Division of Cardiovascular Surgery, 2Department of Neonatology, 3Department of Pediatrics, Japanese Red Cross Medical Center, Shibuya, Tokyo, Japan Abstract: Pharmacological and/or surgical closure of a hemodynamically significant patent ductus arteriosus (PDA in the very premature infant has been the standard of care over the past few decades. However, the rationale for closure of PDA has recently been challenged. In this article, three ways of approaching the closure of PDA including pharmacological treatment, catheter intervention, and surgical intervention, are reviewed in detail. In addition, the different treatment strategies applied in clinical care are evaluated with a focus on the discussion of the available evidence of PDA treatment in the literature. Keywords: patent ductus arteriosus, premature infant, treatment option

  18. Building a Global Responsive Organization:

    DEFF Research Database (Denmark)

    Sun, Xinbo; Cao, Yi; Li, Suxiu

    2017-01-01

    This chapter outlines the philosophic underpinnings of the self-management paradigm developed over the past three decades by China’s Haier Group, a global leader in white goods. The successful transformation of Haier from a small resource-poor firm to a dominant global giant is often attributed...... to the self-management culture established in the company by its legendary leader Zhang Ruimin. This management paradigm is a function of the humbleness displayed by Mr. Zhang Ruimin and rooted in his strong belief in the traditional Chinese philosophy of I-Ching and Daoism. We show how the hexagram of Qian...... (“qian”: humbleness, modesty) from I-Ching is linked to Mr. Zhang’s humble approach and analyze how the six parts of the hexagram of Qian are related to the six development stages of the Haier Group. These insights are used to give some thoughts to the leadership challenge associated with the creation...

  19. Land-total and Ocean-total Precipitation and Evaporation from a Community Atmosphere Model version 5 Perturbed Parameter Ensemble

    Energy Technology Data Exchange (ETDEWEB)

    Covey, Curt [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Lucas, Donald D. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Trenberth, Kevin E. [National Center for Atmospheric Research, Boulder, CO (United States)

    2016-03-02

    This document presents the large scale water budget statistics of a perturbed input-parameter ensemble of atmospheric model runs. The model is Version 5.1.02 of the Community Atmosphere Model (CAM). These runs are the “C-Ensemble” described by Qian et al., “Parametric Sensitivity Analysis of Precipitation at Global and Local Scales in the Community Atmosphere Model CAM5” (Journal of Advances in Modeling the Earth System, 2015). As noted by Qian et al., the simulations are “AMIP type” with temperature and sea ice boundary conditions chosen to match surface observations for the five year period 2000-2004. There are 1100 ensemble members in addition to one run with default inputparameter values.

  20. Response to 'Comment on 'Ultrafast electron optics: Propagation dynamics of femtosecond electron packets'' [J. Appl. Phys. 94, 803 (2003)

    International Nuclear Information System (INIS)

    Siwick, Bradley J.; Dwyer, Jason R.; Jordan, Robert E.; Miller, R. J. Dwayne

    2003-01-01

    In this reply, we address the main issues raised by Qian et al. regarding our recent article [J. Appl. Phys. 92, 1643 (2002)]. In particular, we reiterate the approximations used in the development of the mean-field model and demonstrate how the form used for the on-axis potential is applicable to the study of femtosecond electron packet propagation and is not in need of correction. We also repeat our assertion that the one-dimensional (1-D) fluid model developed by Qian et al. [J. Appl. Phys. 91, 462 (2002)] overestimates space-charge-induced pulse broadening and is in qualitative disagreement with femtosecond electron packet propagation dynamics. The key differences between the mean-field and 1-D fluid model are discussed and their range of applicability is clarified

  1. The Genesis of Cyberscience and its Mathematical Models (CYBERSCIENCE)

    Science.gov (United States)

    2005-02-01

    October 1996. [Gas01] G. Gaskell. An Analysis of BS7799 and Requirements for eCommerce . In Proceedings of the 1st Symposium on Requirements Engineering for...identifying potential vulnerabilities in software. In 1998 IEEE Symposium 70 on Security and Privacy (SSP ’98), pages 104–114, Washington – Brussels...X. Qian, R. Riemenschneider, and L. Gong. Secure Software Architecture. In Proceedings of the 1997 IEEE Symposium on Security and Privacy , pages 84–93

  2. Maritime Issues between Pakistan and India: Seeking Cooperation and Regional Stability

    Science.gov (United States)

    2012-12-01

    avoidance of double taxation & cultural cooperation. 1991 Air Space Violation agreement signed and ratified in August 1992, stipulates that no combat...On July 18, 1994, Foreign Minister Qian Qichen signed another trade agreement on avoidance of double taxation to encourage business, scientific...position that it lacked jurisdiction to entertain the case, because India had filed an exemption in 1974, concerning disputes between India and

  3. Bulletin of Materials Science | Indian Academy of Sciences

    Indian Academy of Sciences (India)

    Home; Journals; Bulletin of Materials Science; Volume 36; Issue 4. Investigation of localization effect in GaN-rich InGaN alloys and modified band-tail model. Chuan-Zhen Zhao Bin Liu De-Yi Fu Hui Chen Ming Li Xiang-Qian Xiu Zi-Li Xie Shu-Lin Gu You-Dou Zheng. Volume 36 Issue 4 August 2013 pp 619-622 ...

  4. Properties of Aerosols during a Dust Storm over the Beijing Area,

    Science.gov (United States)

    1983-12-12

    THE BEIJING AREA by Zhou Mingyu , Qu Shaohou, et al DTIC S ELECTE JAN 04 1984 N Lum E Approved for public release; distribution unlimited. 84 01 04 015...STORM OVER THE BEIJING AREA By: Zhou Mingyu , Qu Shaohou, et al. English~ pages: 22 Source: Huanjing Kexue Xuebao, Vol. 1, Nr. 3, September 1981, pp... Mingyu , Qu Shaohou, Song Ximing, Li Yuying, Yang Shaojin and Qian Qinfang Zhou, Qu, Song and Li of Institute of Atmospheric Physics, Chinese Academy

  5. Distributed Learning, Extremum Seeking, and Model-Free Optimization for the Resilient Coordination of Multi-Agent Adversarial Groups

    Science.gov (United States)

    2016-09-07

    and Technology, UK) OTHER MAJOR RECOGNITIONS 2016 Qian Ren Professor, Northeastern University, China 2015 Invitation Fellow, Japan Society for...Foroush and S. Martı́nez. On triggering control of single-input linear systems under pulse- width modulated DoS jamming attacks. SIAM Journal on...controllable linear systems under unknown periodic DoS jamming attacks. In SIAM Conference on Control and Its Applications (CT), pages 222–229, San Diego, CA

  6. Interactions of bacteria with diatoms: Influence on natural marine biofilms.

    Digital Repository Service at National Institute of Oceanography (India)

    Khandeparker, L.; DeCosta, P.M.; Anil, A.C.; Sawant, S.S.

    therein). In fact, biofilms are considered a reservoir and source of dissemination for V. cholerae (Shikuma & Hadfield 2010). Nutrient concentrations in the surrounding waters also affect the progression of the biofilm community (Qian et al. 2007... to render diatom monocultures near axenic (Patil & Anil 2005c). The following diluents were used – Aged Sea Water (ASW; unenriched control), ASW+streptomycin (ASW+S), ASW+chloramphenicol (ASW+C), f/2 medium (Guillard and Ryther, 1962) prepared in ASW [f...

  7. China Report, Political, Sociological and Military Affairs

    Science.gov (United States)

    1985-07-16

    the dual fermentation method, tech- nology to reclaim sugar residue, technology to make glucose from rice, fructose and dextrose syrup and other...Hu Wenjun [5170 2429 0689], Su Mingkun [5685 2494 0981], Wang Qian [3769 0241] and other conrades to suc- cessfully trial-produce an oral glucose of...company, the comrades praise in unison his high sense of responsibility toward the enterprise and say that old friend Hong is now like a fish in water

  8. Computer Aided Detection of Breast Masses in Digital Tomosynthesis

    Science.gov (United States)

    2008-06-01

    of unknown pathology , all other ROIs generated from that specific subject’s reconstructed volumes were excluded from the KB. For scheme B, all the FPs...query ROI of unknown pathology , all other ROIs generated from that specific subject’s reconstructed volumes were excluded from the KB. For scheme B...Qian, L. Li, and L.P. Clarke, "Image feature extraction for mass detection in digital mammography: Influence of wavelet analysis." Med. Phys. 26

  9. Creative Strategic Intelligence Analysis and Decision Making Within the Elements of National Power. Proteus Futures Workshop, held Carlise, PA on 14-16 Aug 2007

    Science.gov (United States)

    2007-08-01

    since WWI. The emergence of quantitative MOR in China can be traced to a single person, Qian Xuesen. He was born in 1911 and came to the U.S. in 1935...model. A foreign- born student from a nation known to be unfriendly to the United States would receive a higher threat rating than one from a...Kunstler has published numerous journal articles and book chapters on technology, leadership, creatiity, eCommerce , and education and has

  10. EMBROIDERING GUIZHOU

    Institute of Scientific and Technical Information of China (English)

    SUSAN; TRIMBLE

    2004-01-01

    GUIZHOU,known as Qian, is a beautiful province covered in karst formations, primitive forest, numerous sparkling rivers, forests of bamboo, China fir, rung, tea oil, and raw lacquer trees, and intermittently patched with buckwheat fields, rice paddies, vegetable gardens, luscious fruit trees and scented flowering plants and shrubs. Home to waterfalls and canyons, exotic animals, reptiles and birds, and many culturally rich and colorful minority tribes

  11. The Impact of an Entrepreneur's Knowledge - A Case of Nokia's Spinoff

    OpenAIRE

    Olkkonen, Juulia; Tuovinen, Annastina

    2018-01-01

    In recent years the study of entrepreneurial spinoffs has focused on how knowledge spillover leads to new knowledge and entrepreneurial activity. This study aims to explore the effect of a founder’s knowledge inherited from an incumbent parent company in the formation of a spinoff in the ICT sector. As a theoretical framework, the study uses An Absorptive Capacity Theory of Knowledge Spillover Entrepreneurship (Qian and Acs, 2013). Using a qualitative approach through interviews with the foun...

  12. Interoperable Communications for Hierarchical Heterogeneous Wireless Networks

    Science.gov (United States)

    2016-04-01

    International Conference on Advanced Networks and Telecommuncations Systems (ANTS). 14-DEC-13, Kattankulathur, India. : , Husheng Li, Qi Zeng , Lijun Qian. GPS...correlation in space is too large, which implies that the correlation is overestimated. Other methods may be more accurate, faster or less memory ...limited, an intelligent mechanism is needed for the information selection and signaling design of the cross-network communication for collaborative

  13. El multilateralismo estratégico en la política exterior de China.

    Directory of Open Access Journals (Sweden)

    María Florencia Rubiolo.

    2010-07-01

    Full Text Available In 1991, Qian Chen, then Minister of Foreign Relations of the People's Republic of China, attended the ASEAN Ministerial Meeting. This event set the beginning of China's opening to multilateralism in the region. After the financial crisis of 1997, this trend became accentu-ated when Beijing took on a role that involved more leadership and initiative in multilateral bodies. In this article we are interested in exploring the role China plays in strategic multilateral regional initiatives.

  14. Changing patient safety culture in China: a case study of an experimental Chinese hospital from a comparative perspective

    OpenAIRE

    Xu XP; Deng DN; Gu YH; Ng CS; Cai X; Xu J; Zhang XS; Ke DG; Yu QH; Chan CK

    2018-01-01

    Xiao Ping Xu,* Dong Ning Deng,* Yong Hong Gu, Chui Shan Ng, Xiao Cai, Jun Xu, Xin Shi Zhang, Dong Ge Ke, Qian Hui Yu, Chi Kuen Chan Clinical Service Department, The University of Hong Kong - Shenzhen Hospital, Shenzhen, Guangdong, People’s Republic of China *These authors contributed equally to this work Background: The World Health Organization highlights that patient safety interventions are not lacking but that the local context affects their successful implementation. Increasing att...

  15. Physicochemical Properties and Lipophilicity of Polydatin-Lecithin ...

    African Journals Online (AJOL)

    Yang Liu, Baogang Wang, Qian Zhang, Kunfeng Liu, Quanxi Wu ... HPLC analysis found that the solubility of polydatin in n-octanol at 25 °C was enhanced from 0.41 mg/mL to 21.98 mg/mL by complexing with lecithin, indicating that the lipophilicity of polydatin was significantly improved. Conclusion: Polydatin and lecithin in ...

  16. Chinese herbal dose in ancient and modern times: a comparative study.

    Science.gov (United States)

    He, Shimin

    2013-04-01

    Appropriate dose guarantees Chinese herb's safety and effectiveness. There are adult dose criteria for Chinese herbs in decoction in the Pharmacopoeia of the People's Republic of China (2010 Edition) at present. But Chinese herbs have been frequently used in much higher doses than the dose criteria. This study has been conducted to test the dependability of the dose criteria. Twenty Chinese herbs were selected as representatives and their adult doses in decoction in Bei Ji Qian Jin Yao Fang, a famous ancient literature, have been reviewed and compared with those in the Pharmacopoeia of the People's Republic of China (2010 Edition). The adult dose criteria for all these 20 Chinese herbs in the Pharmacopoeia of the People's Republic of China (2010 Edition) haven't covered those in Bei Ji Qian Jin Yao Fang. Furthermore, maximal adult doses in the dose criteria are much lower than those in Bei Ji Qian Jin Yao Fang. The dose criteria in the Pharmacopoeia of the People's Republic of China (2010 Edition) are not comprehensive enough. Studying ancient literatures is an effective method to gain precious Chinese herbs' dose data and helps for new dose criteria's establishment in the future.

  17. PREFACE: IUMRS-ICA 2008 Symposium, Sessions 'X. Applications of Synchrotron Radiation and Neutron Beam to Soft Matter Science' and 'Y. Frontier of Polymeric Nano-Soft-Materials - Precision Polymer Synthesis, Self-assembling and Their Functionalization'

    Science.gov (United States)

    Takahara, Atsushi; Kawahara, Seiichi

    2009-09-01

    Applications of Synchrotron Radiation and Neutron Beam to Soft Matter Science (Symposium X of IUMRS-ICA2008) Toshiji Kanaya, Kohji Tashiro, Kazuo Sakura Keiji Tanaka, Sono Sasaki, Naoya Torikai, Moonhor Ree, Kookheon Char, Charles C Han, Atsushi Takahara This volume contains peer-reviewed invited and contributed papers that were presented in Symposium X 'Applications of Synchrotron Radiation and Neutron Beam to Soft Matter Science' at the IUMRS International Conference in Asia 2008 (IUMRS-ICA 2008), which was held on 9-13 December 2008, at Nagoya Congress Center, Nagoya, Japan. Structure analyses of soft materials based on synchrotron radiation (SR) and neutron beam have been developed steadily. Small-angle scattering and wide-angle diffraction techniques clarified the higher-order structure as well as time dependence of structure development such as crystallization and microphase-separation. On the other hand, reflectivity, grazing-incidence scattering and diffraction techniques revealed the surface and interface structural features of soft materials. From the viewpoint of strong interests on the development of SR and neutron beam techniques for soft materials, the objective of this symposium is to provide an interdisciplinary forum for the discussion of recent advances in research, development, and applications of SR and neutron beams to soft matter science. In this symposium, 21 oral papers containing 16 invited papers and 14 poster papers from China, India, Korea, Taiwan, and Japan were presented during the three-day symposium. As a result of the review of poster and oral presentations of young scientists by symposium chairs, Dr Kummetha Raghunatha Reddy (Toyota Technological Institute) received the IUMRS-ICA 2008 Young Researcher Award. We are grateful to all invited speakers and many participants for valuable contributions and active discussions. Organizing committee of Symposium (IUMRS-ICA 2008) Professor Toshiji Kanaya (Kyoto University) Professor Kohji

  18. A New Stonefly Species, Rhopalopsole tricuspis (Leuctridae: Plecoptera), and Three New Records of Stoneflies from the Qinling Mountains of Shaanxi, China

    Science.gov (United States)

    Qian, Yu-Han; Du, Yu-Zhou

    2012-01-01

    Plecopteran species (Leuctridae) were collected from the Qinling Mountains in southern Shaanxi Province, China. This mountain range is home to nine species of Leuctridae belonging to two genera, and the species identified in this work include one new species and three new records for the Qinling Mountains, all belonging to the genus Rhopalopsole. The new species is named R. tricuspis Qian and Du, sp. nov. A redescription of R. basinigra Yang and Yang 1995 is supplemented. A key is provided for the adult males of Leuctridae from the Qinling Mountains. PMID:22957649

  19. Antifouling sesquiterpene from the Indian soft coral, Sinularia kavarattiensis Alderslade and Prita

    Digital Repository Service at National Institute of Oceanography (India)

    LimnaMol, V.P.; Raveendran, T.V.; Parameswaran, P.S.; Kunnath, R.J.; Sathyan, N.

    analysis of preference data, Appl Entomol Zool, 33 (1998) 339-347. 16 Williams D H & Faulkner D J, Two practical syntheses of an anti-inflammatory sesquiterpene furoic acid from Sinularia spp. Tetrahedron, 52 (1996) 4245-4256. 17 Rittschof D, Lai C H..., Kok L M & Teo S L M, Pharmaceuticals as antifoulants: Concept and principles, Biofouling, 19 (2003) 207-212. 18 Kwong T F N, Miao L, Li X & Qian P Y, Novel Antifouling and Antimicrobial Compound from a Marine-Derived Fungus Ampelomyces sp., Mar...

  20. Stabilization of Gold Nanorods (GNRs) in Aqueous and Organic Environments by Select Surface Functionalization

    Science.gov (United States)

    2016-01-01

    for up to 1 month (the solution was brought to room temperature prior to use). Stock solutions of silver nitrate (AgNO3) were kept at 4 °C and...Qian W, El-Sayed MA. Cancer cell imaging and photothermal therapy in the near-infrared region by using gold nanorods. J Am Chem Soc. 2006;128(6...distribution is unlimited. 16 List of Symbols, Abbreviations, and Acronyms AgNO3 silver nitrate λLSPR wavelength ARL US Army Research Laboratory Au

  1. Power, identity and antiquarian approaches in modern Chinese art

    OpenAIRE

    Yang, Chia-Ling

    2014-01-01

    The pursuit of antiquity was important for scholarly artists in constructing their knowledge of history and cultural identity in late Imperial China. Following various publications by Bi Yuan 畢沅 (1730-1797), Wu Yi 武億 (1745-1799) and Qian Daxin 錢大昕 (1728-1804) in the 18th century, the study and collecting of rubbings of Northern Wei stone inscriptions and steles was popular. Such spread of interest in jinshi, inscriptions on metal and stone, also formed a base for studying seal carving, epigra...

  2. An experimental evaluation of the Gerdemann–Jablonski compaction equation

    CSIR Research Space (South Africa)

    Machaka, R

    2015-05-01

    Full Text Available properties of the material being compacted’ falls short on Denny’s criterion[12] of an ideal multi-stage compaction equation. Thus, the underlying mechanisms driving the compaction process seemingly cannot be described completely in terms of a one...–101. 11. D. Jeyasimman, K. Sivaprasad, S. Sivasankaran, and R. Narayanasamy: Powder Technol., 2014, vol. 258, pp. 189–97. 12. P.J. Denny: Powder Technol., 2002, vol. 127 (2), pp. 162–72. 13. M. Qian: Int. J. Powder Metall., 2010, vol. 46 (5), pp. 29–44. 14...

  3. Research on Foreign Background and Musical Thought of Teng Gu

    Directory of Open Access Journals (Sweden)

    Bo Zhang

    2014-12-01

    Full Text Available TENG Gu is a famous art historian in Republic of China. Together with early Chinese scholars studying abroad like ZONG Bai-hua, ZHU Guang-qian, DENG Yi-zhe, MA Cai and FU Bao-shi, he constructed a framework of early Chinese Art. We still know his influence after a century. In his artistic thought, the essential music idea occupies 1/3.He had given music the highest position among all arts and cultures. In his view, culture won’t have any value if art is removed. And all arts follow the rhythm of music.

  4. Influence of pH on the Transport of Silver Nanoparticles in Saturated Porous Media: Laboratory Experiments and Modeling

    Science.gov (United States)

    2012-03-01

    the potential toxic effects of AgNPs (USEPA 2010). Recent in vitro and in vivo studies using various cell lines, algae , zooplankton, fish, rats and... TiO2 in Saturated Porous Media: Effects of pH, Surfactants and Flow Velocity.” Water Research, 45(2), 839-851. He, F., Zhang, M., Qian, T., and Zhao...of silver nanoparticles (AgNPs), the largest and fastest growing category of nanomaterials, and their potential for toxic effects to both humans

  5. Porous and Phase Change Nanomaterials For Photonic Applications

    Science.gov (United States)

    2014-08-28

    Y. Han , M. S. Zhao, G. Morthier, and R. Baets. Athermal silicon-on-insulator ring resonators by overlaying a polymer cladding on narrowed waveguides...integrated photonics. Advanced Materials, 22(23):2561, 2010. [152] T. Sondergaard, S. M. Novikov, T. Holmgaard, R. L. Eriksen, J. Beermann, Z. H. Han , K...726, 2011. [181] N. N. Feng, D. Z. Feng, S. R. Liao, X. Wang, P. Dong, H. Liang, C. C. Kung , W. Qian, J. Fong, R. Shafiiha, Y. Luo, J. Cunningham, A. V

  6. Gnomon shadow lengths recorded in the Zhoubi Suanjing: the earliest meridian observations in China?

    International Nuclear Information System (INIS)

    Li Yong; Sun Xiaochun

    2009-01-01

    The Zhoubi Suanjing, one of the most important ancient Chinese books on mathematical astronomy, was compiled about 100 BC in the Western Han dynasty (BC 206 - AD 23). We study the gnomon shadow lengths for the 24 solar terms as recorded in the book. Special attention is paid to the so-called law of 'cun qian li', which says the shadow length of a gnomon of 8 chi (about 1.96 m) high will increase (or decrease) 1 cun (1/10 chi) for every 1000 li (roughly 400 km) the gnomon moves northward (or southward). From these data, one can derive the time and location of the observations. The results, however, do not fit historical facts. We suggest that compilers of the Zhoubi Suanjing must have modified the original data according to the law of 'cun qian li'. Through reversing the situation, we recovered the original data, our analysis of which reveals the best possible observation time as 564 BC and the location of observation as 35.78 deg. N latitude. We conclude that this must be the earliest records of solar meridian observations in China. In the meantime, we give the errors of solar altitudes for the 24 solar terms. The average deviation is 5.22 deg., and the mean absolute deviation is 5.52 deg., signifying the accuracy of astronomical calculations from that time.

  7. 15th International Conference on Man–Machine–Environment System Engineering

    CERN Document Server

    Dhillon, Balbir

    2015-01-01

    This research topic was first established in China by Professor ShengZhao Long in 1981, with direct support from one of the greatest modern Chinese scientists, XueSen Qian. In a letter to ShengZhao Long from October 22nd, 1993, XueSen Qian wrote: “You have created a very important modern science subject and technology in China!” MMESE primarily focuses on the relationship between Man, Machine and Environment, studying the optimum combination of man-machine-environment systems. In this system, “Man” refers to working people as the subject in the workplace (e.g. operators, decision-makers); “Machine” is the general name for any object controlled by Man (including tools, machinery, computers, systems and technologies), and “Environment” describes the specific working conditions under which Man and Machine interact (e.g. temperature, noise, vibration, hazardous gases etc.). The three goals of optimization are to ensure safety, efficiency and economy. These proceedings are an academic showcase of t...

  8. 13th International Conference on Man-Machine-Environment System Engineering

    CERN Document Server

    Dhillon, Balbir

    2014-01-01

    The integrated and advanced science research topic Man-Machine-Environment System Engineering (MMESE) was first established in China by Professor Shengzhao Long in 1981, with direct support from one of the greatest modern Chinese scientists, Xuesen Qian. In a letter to Shengzhao Long from October 22nd, 1993, Xuesen Qian wrote: “You have created a very important modern science and technology in China!”   MMESE primarily focuses on the relationship between man, machines and the environment, studying the optimum combination of man-machine-environment systems. In this system, “man” refers to people in the workplace (e.g. operators, decision-makers); “ machine” is the general name for any object controlled by man (including tools, machinery, computers, systems and technologies), and “environment” describes the specific working conditions under which man and machine interact (e.g. temperature, noise, vibration, hazardous gases etc.). The three goals of optimization of Man-Machine-Environment system...

  9. 16th International Conference on Man-Machine-Environment System Engineering

    CERN Document Server

    Dhillon, Balbir

    2016-01-01

    This research topic was first established in China by Professor Shengzhao Long in 1981, with direct support from one of the greatest modern Chinese scientists, Xuesen Qian. In a letter to Shengzhao Long from October 22nd, 1993, Xuesen Qian wrote: “You have created a very important modern science subject and technology in China!” MMESE primarily focuses on the relationship between Man, Machine and Environment, studying the optimum combination of man-machine-environment systems. In this system, “Man” refers to working people as the subject in the workplace (e.g. operators, decision-makers); “Machine” is the general name for any object controlled by Man (including tools, machinery, computers, systems and technologies), and “Environment” describes the specific working conditions under which Man and Machine interact (e.g. temperature, noise, vibration, hazardous gases etc.). The three goals of optimization are to ensure "Safety, High efficiency and Economy" of man-machine-environment systems. These...

  10. 14th International Conference on Man-Machine-Environment System Engineering

    CERN Document Server

    Dhillon, Balbir

    2015-01-01

    The integrated and advanced science research topic man-machine-environment system engineering (MMESE) was first established in China by Professor Shengzhao Long in 1981, with direct support from one of the greatest modern Chinese scientists, Xuesen Qian. In a letter to Shengzhao Long from October 22nd, 1993, Xuesen Qian wrote: “You have created a very important modern science and technology in China!”   MMESE primarily focuses on the relationship between man, machines and the environment, studying the optimum combination of man-machine-environment systems. In this system, “man” refers to people in the workplace (e.g. operators, decision-makers); “ machine” is the general name for any object controlled by man (including tools, machinery, computers, systems and technologies), and “environment” describes the specific working conditions under which man and machine interact (e.g. temperature, noise, vibration, hazardous gases etc.). The three goals of optimization of man-machine-environment system...

  11. Molecular cloning, polymorphisms, and expression analysis of the RERG gene in indigenous Chinese goats.

    Science.gov (United States)

    Sui, M X; Wang, H H; Wang, Z W

    2015-11-24

    The current study aimed to investigate the coding sequence, polymorphisms, and expression of the RERG gene in indigenous Chinese goats. cDNA of RERG, obtained through reverse transcription PCR was analyzed using bioinformatic techniques. Polymorphisms in the exon regions of the RERG gene were identified and their associations with growth traits in three varieties of indigenous Chinese goats were investigated. Expression of the RERG gene in three goat breeds of the same age was detected using real-time quantitative PCR. The results revealed that the cDNA of RERG, which contained a complete open reading frame of 20-620 bp, was 629 bp in length. The associated accession numbers in GenBank are JN672576, JQ917222, and JN580309 for the QianBei Ma goat, the GuiZhou white goat, and the GuiZhou black goat, respectively. Four consistent SNP sites were found in the exon regions of the RERG gene for the three goat breeds. mRNA expression of the RERG gene differed between different tissues in adult goats of same age. The highest expression was observed in lung and spleen tissues, while the lowest expression was recorded in thymus gland tissue. In addition, the expression of the RERG gene in the muscle of Guizhou white goat, GuiZhou black goat, and QianBei Ma goat decreased sequentially. Our results lay the foundations for further investigation into the role of the RERG gene in goat growth traits.

  12. Medical irradiation technique saves lives of victims of Lima inferno

    International Nuclear Information System (INIS)

    2002-01-01

    Full text: Nearly 400 people died when fire swept through a crowded market place in the old centre of Lima, Peru, as the city readied for New Year's Eve, 2001. Yet the lives of more than 60 severely burned people were saved because Peru belongs to a programme, initiated and supported by the International Atomic Energy Agency (IAEA), that permits developing countries to maintain their own tissue banks. 'Tissue grafts are routine treatment in developed countries, said Qian Jihui, Deputy Director General and head of the IAEA Department of Technical Cooperation. 'This programme is saving lives and improving significantly the quality of lives for patients in developing countries.' More than 1600 radiation sterilized tissue packs were provided by Peru's atomic energy institute to eight Lima hospitals and clinics, where nurses and doctors battled to help the fire victims, many of them poor market traders. Peru and six other countries in the IAEA Latin America programme offer patients irradiated sterile human skin and bone from 37 tissue banks for transplant use. IAEA support of tissue banking began in 1983, since when the agency has contributed US $5,313,335 to the programme, resulting in the establishment of 66 tissue banks in the Asia Pacific region, seven in Africa, while the number is growing. Tissue grafts increase the odds for burn patients' survival by reducing the risk of infection. They are also used to treat victims of leprosy. A bone transplant can save a limb. The IAEA has helped develop a system - used in 28 countries - that disinfects tissue grafts in the final package with ionising radiation without damaging them, dramatically lowering the risk of contamination. Now medical authorities in the United States are considering adoption of similar irradiation techniques, following a number of cases of contamination of medical tissue used for transplant. The issue will be among items to be discussed in Vienna, December 2-4, at IAEA headquarters, at a meeting of

  13. Critical analysis of the potential for therapeutic targeting of mammalian target of rapamycin (mTOR in gastric cancer

    Directory of Open Access Journals (Sweden)

    Inokuchi M

    2014-04-01

    Full Text Available Mikito Inokuchi,1 Keiji Kato,1 Kazuyuki Kojima,2 Kenichi Sugihara1 1Department of Surgical Oncology, 2Department of Minimally Invasive Surgery, Tokyo Medical and Dental University, Tokyo, Japan Abstract: Multidisciplinary treatment including chemotherapy has become the global standard of care for patients with metastatic gastric cancer (mGC; nonetheless, survival remains poor. Although many molecular-targeted therapies have been developed for various cancers, only anti-HER2 treatment has produced promising results in patients with mGC. Mammalian target of rapamycin (mTOR plays a key role in cell proliferation, antiapoptosis, and metastasis in signaling pathways from the tyrosine kinase receptor, and its activation has been demonstrated in gastric cancer (GC cells. This review discusses the clinical relevance of mTOR in GC and examines its potential as a therapeutic target in patients with mGC. Preclinical studies in animal models suggest that suppression of the mTOR pathway inhibits the proliferation of GC cells and delays tumor progression. The mTOR inhibitor everolimus has been evaluated as second- or third-line treatment in clinical trials. Adverse events were well tolerated although the effectiveness of everolimus alone was limited. Everolimus is now being evaluated in combination with chemotherapy in Phase III clinical studies in this subgroup of patients. Two Phase III studies include exploratory biomarker research designed to evaluate the predictive value of the expression or mutation of molecules related to the Akt/mTOR signaling pathway. These biomarker studies may lead to the realization of targeted therapy for selected patients with mGC in the future. Keywords: gastric cancer, mTOR, everolimus

  14. Influence of container structures and content solutions on dispensing time of ophthalmic solutions

    Directory of Open Access Journals (Sweden)

    Keiji Yoshikawa

    2010-05-01

    Full Text Available Keiji Yoshikawa1, Hiroshi Yamada21Yoshikawa Eye Clinic, Tokyo, Japan; 2Santen Pharmaceutical Co., Ltd., Osaka, JapanPurpose: To investigate the influence of container structures and content solutions on the time of dispensing from eye dropper bottles.Methods: Eye dropper bottle models, solution models (filtrate water/surfactant solution and a dispensing time measuring apparatus were prepared to measure the dispensing time.Results: With filtrate water and pressure thrust load of 0.3 MPa, the dispensing time significantly increased from 1.1 ± 0.5 seconds to 4.6 ± 1.1 seconds depending on the decrease of inner aperture diameters from 0.4 mm to 0.2 mm (P < 0.0001. When using the bottle models with inner aperture diameters of 0.4 mm or larger, the dispensing time became constant. The dispensing time using surfactant solution showed the same tendency as above. When pressure thrust load was large (0.07 MPa, the solution flew out continuously with inner aperture diameters of 0.4 mm or larger and the dispensing time could not be measured. The inner aperture diameter most strongly explained the variation of the dispensing time in both the content solutions in the multiple linear regression analysis (filtrate water: 46%, R2 = 0.462, surfactant solution: 56%, R2 = 0.563.Conclusions: Among content solutions and container structures, the dispensing time was mostly influenced by the diameter of the inner aperture of bottles.Keywords: dispensing time, model eye dropper bottle, model ophthalmic solution, nozzle internal space volume, nozzle inner aperture diameter

  15. Managing refractory Crohn's disease: challenges and solutions

    Directory of Open Access Journals (Sweden)

    Tanida S

    2015-04-01

    Full Text Available Satoshi Tanida, Keiji Ozeki, Tsutomu Mizoshita, Hironobu Tsukamoto, Takahito Katano, Hiromi Kataoka, Takeshi Kamiya, Takashi Joh Department of Gastroenterology and Metabolism, Nagoya City University Graduate School of Medical Sciences, Aichi Prefecture, Japan Abstract: The goals of treatment for active Crohn's disease (CD are to achieve clinical remission and improve quality of life. Conventional therapeutics for moderate-to-severe CD include 5-aminosalicylic acid, corticosteroids, purine analogs, azathioprine, and 6-mercaptopurine. Patients who fail to respond to conventional therapy are treated with tumor necrosis factor (TNF-α inhibitors such as infliximab and adalimumab, but their efficacy is limited due to primary nonresponse or loss of response. It is suggested that this requires switch to another TNF-α inhibitor, a combination therapy with TNF-α blockade plus azathioprine, or granulocyte and monocyte adsorptive apheresis, and that other therapeutic options having different mechanisms of action, such as blockade of inflammatory cytokines or adhesion molecules, are needed. Natalizumab and vedolizumab are neutralizing antibodies directed against integrin a4 and a4ß7, respectively. Ustekinumab is a neutralizing antibody directed against the receptors for interleukin-12 and interleukin-23. Here, we provide an overview of therapeutic treatments that are effective and currently available for CD patients, as well as some that likely will be available in the near future. We also discuss the advantages of managing patients with refractory CD using a combination of TNF-α inhibitors plus azathioprine or intensive monocyte adsorptive apheresis. Keywords: adalimumab, granulocyte and monocyte adsorptive apheresis, combination therapy, complete remission

  16. Zhouyi's Influence to the Shape of Characters of Records of the Historian%《周易》对《史记》人物形象塑造的影响

    Institute of Scientific and Technical Information of China (English)

    吴章燕

    2012-01-01

    司马迁创作《史记》的思想深受《易》理的影响,《史记》的人物形象塑造是其《易》理思想的具体体现。本文从人物典型化手法、含蓄化手法、互见法、悲剧人物、传奇色彩五个方面展开论述,说明《周易》与《史记》二者之间存在密切关系。%Sima-qian works, Records of the Historian, had been influenced deeply by Zhouyi. The characters' shape in Records of the Historian is specific embodiment of Zhouyi. This paper will discuss relationship between Records of the Historian and Zhouyi from five aspects such as character typical technique, the implicit technique, the apparent method, tragic, and legendary characters.

  17. Between the Living and the Dead: Trauma Medicine and Forensic Medicine in the Mid-Qing.

    Science.gov (United States)

    Wu, Yi-Li

    2015-03-01

    This paper analyzes the influence of forensic medicine on therapeutic medicine through a case study of Qian Xiuchang and Hu Tingguang, two Chinese doctors who specialized in treating traumatic injuries. During the early nineteenth century, both men compiled medical treatises that sought to improve on a scholarly model of "rectifying bones" articulated in 1742 by the Imperially-Compiled Golden Mirror of the Medical Lineage . Both texts also incorporated information from forensic medicine, including official inquest diagrams and checklists promulgated by the Qing government. I show that they drew on these forensic materials to help address two interlinked medical issues: understanding the effects of injury on different parts of the body, and clarifying the location and form of the body's bones. Overall, I suggest that the exchange of ideas between the realm of therapeutic medicine and forensic medicine was an important epistemological strategy that doctors and officials alike employed to improve their knowledge of the material body.

  18. Interactive effects of gallic/ferulic/caffeic acids and anthocyanins on pigment thermal stabilities.

    Science.gov (United States)

    Qian, Bing-Jun; Liu, Jian-Hua; Zhao, Shu-Juan; Cai, Jian-Xiong; Jing, Pu

    2017-06-01

    The data presented in this article are related to the research article entitled "The effects of gallic/ferulic/caffeic acids on colour intensification and anthocyanin stability" (Qian et al., 2017) [1]. This paper described preparation and isolation of anthocyanins from purple sweet potatoes (PSP) and the time-course of anthocyanin profiles treated with gallic, ferulic, or caffeic acids at 95 °C. The color appearance of PSPanthocyanins alone, or with gallic, ferulic, or caffeic acids was described after the 15 h of thermal treatment. The high resolution mass spectrographs of PSP anthocyanins were determined using UPLC-ESI-HRMS. The spatial interaction of peonidin 3-O-(2-O-β-D-glucopyranocyl-β-D-glucopyranoide)-5-O-β-D-glucopyranoside and gallic/ferulic/caffeic acids was illustrated by molecular dynamic simulation.

  19. 26th Conference of Spacecraft TT&C Technology in China

    CERN Document Server

    Qian, Weiping

    2013-01-01

    Proceedings of the 26th Conference of Spacecraft TT&C Technology in China collects selected papers from the 26th Conference of Spacecraft TT&C Technology in China held in Nanjing on October 16-19, 2012. The book features state-of-the-art studies on spacecraft TT&C in China with the theme of “Shared and Flexible TT&C Systems”. The selected works can help  promote development of spacecraft TT&C technology towards interconnectivity, resource sharing, flexibility and high efficiency. Researchers and engineers in the field of aerospace engineering and communication engineering can benefit from the book. Rongjun Shen is the Academician of Chinese Academy of Engineering; Weiping Qian is the Director General of Beijing Institute of Tracking and Telecommunications Technology.

  20. [The modern TCM education of Tianjin].

    Science.gov (United States)

    Xie, J

    2016-11-28

    School education had gradually become the mainstream of medical education at the turn of the Qing Dynasty and Republican period. In Tianjin, there were western medical education, including the Peiyang Medical College, Army Medical College, and Lu's Medical School, whereas, there was no traditional medical school at all. To check this defect, two private TCM medical schools, the Learning School of TCM, and the Tianjin TCM Institution were set up by Chen Zedong and Gu Jinren respectively through persistent endeavor. Meanwhile, correspondence school of TCM were established by Zhang Xichun, Wei Jiaqian, and Ma Leshan. Though more TCM schools were planned, including Tianjin Private School of TCM by Lu Zongren and Qian Boquan, North China Professional TCM School by Zhang Yinqi and other colleagues, TCM Professional School by Zhang Dianju and the Tianjin Private Acu-Moxibustion School by Zhang Lihui, etc. All these did make their contributions to the training of TCM talents in modern times.

  1. Vulnerabilities Classification for Safe Development on Android

    Directory of Open Access Journals (Sweden)

    Ricardo Luis D. M. Ferreira

    2016-06-01

    Full Text Available The global sales market is currently led by devices with the Android operating system. In 2015, more than 1 billion smartphones were sold, of which 81.5% were operated by the Android platform. In 2017, it is estimated that 267.78 billion applications will be downloaded from Google Play. According to Qian, 90% of applications are vulnerable, despite the recommendations of rules and standards for the safe software development. This study presents a classification of vulnerabilities, indicating the vulnerability, the safety aspect defined by the Brazilian Association of Technical Standards (Associação Brasileira de Normas Técnicas - ABNT norm NBR ISO/IEC 27002 which will be violated, which lines of code generate the vulnerability and what should be done to avoid it, and the threat agent used by each of them. This classification allows the identification of possible points of vulnerability, allowing the developer to correct the identified gaps.

  2. ACCORDING TO HWC ON TURKISH WORDS ADOPTED FROM CHINE LANGUAGE / HANYU WAILAICI CIDIAN’a (HWC GÖRE ÇİNCEYE GEÇEN TÜRKÇE KELİMELER ÜZERİNE

    Directory of Open Access Journals (Sweden)

    Prof. Dr. Alimcan İNAYET

    2008-02-01

    Full Text Available Today, there exist thousands of loan words in Chinese language. Chinese linguists -Liu Zheng-yan, Gao Ming-kai, Mai Yong-qian and Shi You-wei- have determined ten thousand loan-words (with the different versions of the same word adopted by Chinese from various languages and published them in “A Dictionary of Loan Words and Hybrid Words in Chinese” in 1984. Among the considered words of this dictionary, there are also hundreds of words which were directly and indirectly adopted from Turkish language and Turkic dialects in various eras. Additionally, some of them are still be in use in Chinese language today. In this article, we have listed those Turkish words determined in this dictionary with the necessary explanations attached.

  3. Learning Matlab a problem solving approach

    CERN Document Server

    Gander, Walter

    2015-01-01

    This comprehensive and stimulating introduction to Matlab, a computer language now widely used for technical computing, is based on an introductory course held at Qian Weichang College, Shanghai University, in the fall of 2014.  Teaching and learning a substantial programming language aren’t always straightforward tasks. Accordingly, this textbook is not meant to cover the whole range of this high-performance technical programming environment, but to motivate first- and second-year undergraduate students in mathematics and computer science to learn Matlab by studying representative problems, developing algorithms and programming them in Matlab. While several topics are taken from the field of scientific computing, the main emphasis is on programming. A wealth of examples are completely discussed and solved, allowing students to learn Matlab by doing: by solving problems, comparing approaches and assessing the proposed solutions.

  4. Issues surrounding end-of-life decision-making

    Directory of Open Access Journals (Sweden)

    Tejwani V

    2013-08-01

    Full Text Available Vickram Tejwani,1,* YiFan Wu,1,* Sabrina Serrano,2 Luis Segura,2 Michael Bannon,3 Qi Qian1 1Department of Medicine, Division of Nephrology and Hypertension, 2Mayo Graduate School, 3Department of Trauma, Critical Care, and General Surgery, Mayo Clinic College of Medicine, Rochester, MN, USA *These authors contributed equally to this work Abstract: End-of-life decision-making is a complex process that can be extremely challenging. We describe a 42-year-old woman in an irreversible coma without an advance directive. The case serves to illustrate the complications that can occur in end-of-life decision-making and challenges in resolving difficult futility disputes. We review the role of advance directives in planning end-of-life care, the responsibility and historical performance of patient surrogates, the genesis of futility disputes, and approaches to resolving disputes. Keywords: end-of-life care, advance directive, surrogate, futility dispute, conflict resolution

  5. Self-sustained oscillations of complex genomic regulatory networks

    International Nuclear Information System (INIS)

    Ye Weiming; Huang Xiaodong; Huang Xuhui; Li Pengfei; Xia Qinzhi; Hu Gang

    2010-01-01

    Recently, self-sustained oscillations in complex networks consisting of non-oscillatory nodes have attracted great interest in diverse natural and social fields. Oscillatory genomic regulatory networks are one of the most typical examples of this kind. Given an oscillatory genomic network, it is important to reveal the central structure generating the oscillation. However, if the network consists of large numbers of genes and interactions, the oscillation generator is deeply hidden in the complicated interactions. We apply the dominant phase-advanced driving path method proposed in Qian et al. (2010) to reduce complex genomic regulatory networks to one-dimensional and unidirectionally linked network graphs where negative regulatory loops are explored to play as the central generators of the oscillations, and oscillation propagation pathways in the complex networks are clearly shown by tree branches radiating from the loops. Based on the above understanding we can control oscillations of genomic networks with high efficiency.

  6. Age as a risk factor for acute mountain sickness upon rapid ascent to 3,700 m among young adult Chinese men

    Directory of Open Access Journals (Sweden)

    Tang XG

    2014-08-01

    Full Text Available Xu-gang Tang,1 Ji-hang Zhang,1 Jun Qin,1 Xu-bin Gao,1 Qian-ning Li,2 Jie Yu,1 Xiao-han Ding,1 Lan Huang1 1Institute of Cardiovascular Diseases, 2Department of Neurology, Xinqiao Hospital, Third Military Medical University, Chongqing, People’s Republic of China Background: The aim of this study was to explore the relationship between age and acute mountain sickness (AMS when subjects are exposed suddenly to high altitude.Methods: A total of 856 young adult men were recruited. Before and after acute altitude exposure, the Athens Insomnia Scale score (AISS was used to evaluate the subjective sleep quality of subjects. AMS was assessed using the Lake Louise scoring system. Heart rate (HR and arterial oxygen saturation (SaO2 were measured.Results: Results showed that, at 500 m, AISS and insomnia prevalence were higher in older individuals. After acute exposure to altitude, the HR, AISS, and insomnia prevalence increased sharply, and the increase in older individuals was more marked. The opposite trend was observed for SaO2. At 3,700 m, the prevalence of AMS increased with age, as did severe AMS, and AMS symptoms (except gastrointestinal symptoms. Multivariate logistic regression analysis showed that age was a risk factor for AMS (adjusted odds ratio [OR] 1.07, 95% confidence interval [CI] 1.01–1.13, P<0.05, as well as AISS (adjusted OR 1.39, 95% CI 1.28–1.51, P<0.001.Conclusion: The present study is the first to demonstrate that older age is an independent risk factor for AMS upon rapid ascent to high altitude among young adult Chinese men, and pre-existing poor subjective sleep quality may be a contributor to increased AMS prevalence in older subjects. Keywords: acute mountain sickness, age, Athens Insomnia Scale, rapid ascent, sleep

  7. Human cytotoxic T-lymphocyte membrane-camouflaged nanoparticles combined with low-dose irradiation: a new approach to enhance drug targeting in gastric cancer

    Directory of Open Access Journals (Sweden)

    Zhang L

    2017-03-01

    Full Text Available Lianru Zhang, Rutian Li, Hong Chen, Jia Wei, Hanqing Qian, Shu Su, Jie Shao, Lifeng Wang, Xiaoping Qian, Baorui Liu The Comprehensive Cancer Centre of Drum Tower Hospital, Medical School of Nanjing University and Clinical Cancer Institute of Nanjing University, Nanjing, People’s Republic of China Abstract: Cell membrane-derived nanoparticles are becoming more attractive because of their ability to mimic many features of their source cells. This study reports on a biomimetic delivery platform based on human cytotoxic T-lymphocyte membranes. In this system, the surface of poly-lactic-co-glycolic acid nanoparticles was camouflaged using T-lymphocyte membranes, and local low-dose irradiation (LDI was used as a chemoattractant for nanoparticle targeting. The T-lymphocyte membrane coating was verified using dynamic light scattering, transmission electron microscopy, and confocal laser scanning microscopy. This new platform reduced nanoparticle phagocytosis by macrophages to 23.99% (P=0.002. Systemic administration of paclitaxel-loaded T-lymphocyte membrane-coated nanoparticles inhibited the growth of human gastric cancer by 56.68% in Balb/c nude mice. Application of LDI at the tumor site significantly increased the tumor growth inhibition rate to 88.50%, and two mice achieved complete remission. Furthermore, LDI could upregulate the expression of adhesion molecules in tumor vessels, which is important in the process of leukocyte adhesion and might contribute to the localization of T-lymphocyte membrane-encapsulated nanoparticles in tumors. Therefore, this new drug-delivery platform retained both the long circulation time and tumor site accumulation ability of human cytotoxic T lymphocytes, while local LDI could significantly enhance tumor localization. Keywords: cell membrane, drug delivery system, gastric cancer, low-dose irradiation, nanoparticles

  8. Gambogic acid-loaded biomimetic nanoparticles in colorectal cancer treatment

    Directory of Open Access Journals (Sweden)

    Zhang Z

    2017-02-01

    Full Text Available Zhen Zhang,1 Hanqing Qian,2 Mi Yang,2 Rutian Li,2 Jing Hu,1 Li Li,1 Lixia Yu,2 Baorui Liu,1,2 Xiaoping Qian1,2 1Comprehensive Cancer Center, Nanjing Drum Tower Hospital Clinical College of Traditional Chinese and Western Medicine, Nanjing University of Traditional Chinese Medicine, 2Comprehensive Cancer Center, Nanjing Drum Tower Hospital, Medical School of Nanjing University, Clinical Cancer Institute, Nanjing University, Nanjing, China Abstract: Gambogic acid (GA is expected to be a potential new antitumor drug, but its poor aqueous solubility and inevitable side effects limit its clinical application. Despite these inhe­rent defects, various nanocarriers can be used to promote the solubility and tumor targeting of GA, improving antitumor efficiency. In addition, a cell membrane-coated nanoparticle platform that was reported recently, unites the customizability and flexibility of a synthetic copolymer, as well as the functionality and complexity of natural membrane, and is a new synthetic biomimetic nanocarrier with improved stability and biocompatibility. Here, we combined poly(lactic-co-glycolic acid (PLGA with red blood-cell membrane (RBCm, and evaluated whether GA-loaded RBCm nanoparticles can retain and improve the antitumor efficacy of GA with relatively lower toxicity in colorectal cancer treatment compared with free GA. We also confirmed the stability, biocompatibility, passive targeting, and few side effects of RBCm-GA/PLGA nanoparticles. We expect to provide a new drug carrier in the treatment of colorectal cancer, which has strong clinical application prospects. In addition, the potential antitumor drug GA and other similar drugs could achieve broader clinical applications via this biomimetic nanocarrier. Keywords: gambogic acid, nanocarriers, RBCm-GA/PLGA nanoparticles, colorectal cancer

  9. A single-arm Phase II validation study of preventing oxaliplatin-induced hypersensitivity reactions by dexamethasone: the AVOID trial

    Directory of Open Access Journals (Sweden)

    Yoshida Y

    2015-11-01

    Full Text Available Yoichiro Yoshida,1 Keiji Hirata,2 Hiroshi Matsuoka,3 Shigeyoshi Iwamoto,4 Masahito Kotaka,5 Hideto Fujita,6 Naoya Aisu,1 Seiichiro Hoshino,1 Takeo Kosaka,6 Kotaro Maeda,3 Fumiaki Kiyomi,7 Yuichi Yamashita1 1Department of Gastroenterological Surgery, Fukuoka University Faculty of Medicine, Fukuoka, Japan; 2Department of Surgery, Fukuoka Sanno Hospital, Fukuoka, Japan; 3Department of Surgery, Fujita Health University School of Medicine, Toyoake, Japan; 4Department of Surgery, Kansai Medical University Hirakata Hospital, Osaka, Japan; 5Gastrointestinal Cancer Center, Sano Hospital, Kobe, Japan; 6Department of Surgical Oncology, Kanazawa Medical University, Uchinada, Japan; 7Academia, Industry and Government Collaborative Research Institute of Translational Medicine for Life Innovation, Fukuoka University, Fukuoka, Japan Background: Patients with colorectal cancer treated with oxaliplatin are at risk of hypersensitivity reactions, with the incidence estimated to be 12%–20%. Coinfusion of dexamethasone and oxaliplatin could potentially reduce the incidence of these reactions, but oxaliplatin is reported to be incompatible with alkaline compounds in solution. However, in a previous retrospective study we found that the pH of a solution of dexamethasone and oxaliplatin was less than 7.4, and that hypersensitivity to oxaliplatin could have been prevented by coinfusion of dexamethasone. We aimed to evaluate the effectiveness of coinfusion of dexamethasone and oxaliplatin to prevent oxaliplatin-induced hypersensitivity reactions.Patients and methods: The AVOID trial was a prospective, multicenter, open-label, single-arm Phase II trial conducted from January to September 2013. The study included 73 patients who received capecitabine plus oxaliplatin (XELOX or XELOX plus bevacizumab therapy for colorectal cancer. In all patients, oxaliplatin was administered in combination with dexamethasone. The primary outcome measure was the presence of

  10. Assessment of retinal sensitivity using a time-saving strategy in normal individuals

    Directory of Open Access Journals (Sweden)

    Suzumura H

    2012-11-01

    Full Text Available Hirotaka Suzumura,1 Keiji Yoshikawa,2 Shiro Mizoue,3 Ryoko Hyodo,4 Tairo Kimura5 1Eye Department, Nakano General Hospital, Tokyo, 2Yoshikawa Eye Clinic, Tokyo, 3Department of Ophthalmology, Ehime University, Ehime, 4Eye Department, Minami Matsuyama Hospital, Ehime, 5Ueno Eye Clinic, Tokyo, JapanBackground: The purpose of this study was to compare retinal sensitivities in normal individuals obtained using the Swedish Interactive Threshold Algorithm Standard (SITA-S on the Humphrey field analyzer with those obtained using the Dynamic strategy on the Octopus.Methods: Prior to visual field examinations, the background luminance, stimulus size, and exposure time with the Octopus 101 were conformed to the Humphrey field analyzer II settings. Volunteers over 20 years of age without apparent ophthalmic abnormalities were examined with the SITA-S central 30-2 program followed by the Dynamic 32 program. Eye with corrected visual acuity ≥0.8, refraction ≥ −6.0 diopters, and fields with satisfactory levels of reliability in SITA-S and Dynamic were selected.Results: Sixty-seven eyes from 67 normal individuals of mean age 51.3 ± 16.3 (range 22–76 years satisfied the selection criteria and were analyzed. Mean retinal sensitivity was significantly (P < 0.0001 higher with SITA-S (29.0 ± 2.4 dB than with Dynamic (26.8 ± 2.1 dB. Changes in retinal sensitivity with increasing age were significantly (P = 0.0003 greater with Dynamic (−0.09 ± 0.04 dB/year; 95% confidence interval [CI] −0.10 to −0.08 dB/year than with SITA-S (−0.07 ± 0.04 dB/year, 95% CI −0.08 to −0.06 dB/year. When classifying the visual field into three areas (central, mid-peripheral, and peripheral, retinal sensitivities with SITA-S were significantly higher in all areas than with Dynamic (P < 0.0001 for all three areas.Conclusion: Differences in Dynamic and SITA-S strategies may contribute to the differences in retinal sensitivities observed in normal individuals

  11. Relationship between consecutive deterioration of mean deviation value and progression of visual field defect in open-angle glaucoma

    Directory of Open Access Journals (Sweden)

    Naito T

    2015-11-01

    Full Text Available Tomoko Naito,1 Keiji Yoshikawa,2 Shiro Mizoue,3 Mami Nanno,4 Tairo Kimura,5 Hirotaka Suzumura,6 Ryuji Takeda,7 Fumio Shiraga1 1Department of Ophthalmology, Okayama University Graduate School of Medicine, Okayama, 2Yoshikawa Eye Clinic, Tokyo, 3Department of Ophthalmology, Ehime University Graduate School of Medicine, Ehime, 4Kagurazaka Minamino Eye Clinic, 5Ueno Eye Clinic, 6Suzumura Eye Clinic, Tokyo, 7Department of Agriculture, Kinki University, Nara, Japan Purpose: To analyze the relationship between consecutive deterioration of mean deviation (MD value and glaucomatous visual field (VF progression in open-angle glaucoma (OAG, including primary OAG and normal tension glaucoma.Patients and methods: The subjects of the study were patients undergoing treatment for OAG who had performed VF tests at least 10 times with a Humphrey field analyzer (SITA standard, C30-2 program. The VF progression was defined by a significantly negative MD slope (MD slope worsening at the final VF test during the follow-up period. The relationship between the MD slope worsening and the consecutive deterioration of MD value were retrospectively analyzed.Results: A total of 165 eyes of 165 patients were included in the analysis. Significant progression of VF defects was observed in 72 eyes of 72 patients (43.6%, while no significant progression was evident in 93 eyes of 93 patients (56.4%. There was significant relationship between the frequency of consecutive deterioration of MD value and MD slope worsening (P<0.0001, Cochran–Armitage trend test. A significant association was observed for MD slope worsening in the eyes with three (odds ratio: 2.1, P=0.0224 and four (odds ratio: 3.6, P=0.0008 consecutive deterioration of MD value in multiple logistic regression analysis, but no significant association in the eyes with two consecutive deterioration (odds ratio: 1.1, P=0.8282. The eyes with VF progression had significantly lower intraocular pressure reduction rate (P<0

  12. Travoprost with sofZia® preservative system lowered intraocular pressure of Japanese normal tension glaucoma with minimal side effects

    Directory of Open Access Journals (Sweden)

    Mizoue S

    2014-02-01

    Full Text Available Shiro Mizoue,1 Tadashi Nakano,2 Nobuo Fuse,3 Aiko Iwase,4 Shun Matsumoto,5 Keiji Yoshikawa6 On behalf of the IOP CHANGE Study Group7 1Department of Ophthalmology, Ehime University Graduate School of Medicine, Ehime, Japan; 2Department of Ophthalmology, Jikei University School of Medicine, Tokyo, Japan; 3Department of Integrative Genomics, Tohoku Medical Megabank Organization, Miyagi, Japan; 4Tajimi Iwase Eye Clinic, Gifu, Japan; 5Department of Ophthalmology, Tokyo Teishin Hospital, 6Yoshikawa Eye Clinic, Tokyo, Japan; 7IOP CHecked and Assessed in Normal tension Glaucoma by Exceptional Glaucomatologists Study Group, Japan Background: This study aimed to evaluate the effect of travoprost with sofZia® preservative system for lowering the intraocular pressure (IOP of Japanese normal tension glaucoma (NTG patients. Methods: In this prospective, multicenter, open-label study, Japanese NTG patients with baseline IOPs <20 mmHg were enrolled after three consecutive time measurements taken at screening and baseline visits. Travoprost with sofZia® was instilled once daily. Lowering effect on IOP, conjunctival hyperemia, superficial punctate keratopathy, and adverse events were examined at week 4, 8, and 12 after drug instillation. Results: One-hundred and three of the 107 enrolled patients (baseline IOP =15.2±2.0 mmHg [mean ± standard deviation] completed the study. The mean IOP value as well as percent reduction was significantly reduced at each visit after travoprost with sofZia® initiation (P<0.0001. The conjunctival hyperemia score was 1 or less throughout the study, though it increased significantly over time. No significant change was observed in superficial punctate keratopathy. The cumulative incidence of side effects such as eyelash changes, eyelid pigmentation, and deepening of the upper lid was 47.6%, 27.2%, and 16.5%, respectively. Conclusion: Travoprost preserved with sofZia® effectively lowered the IOP of Japanese NTG patients. It was

  13. Review and Reflection on the Religious Studies of the Qiang during the Past One Hundred Years

    Institute of Scientific and Technical Information of China (English)

    YUAN Linrong

    2014-01-01

    religious life in the Taoping community of Lixian .In looking at the content , the author did not provide a description of the collective religious activities .Moreover , the book did not mention an-ything about the stability and change of the reli-gious activities after the 1950s, nor did it explain how much or what part of traditional religious fea-tures were still preserved by the Qiang . 4 ) Commercialization of white stone worship The Review of Qiang Religious Studies by Ma Ning and Qian Yongping considers the many tourism companies that hire Qiang shamans to engage in the religious activities in order to attract tourists in the 21st century .Although the income of the shamans increased , their social prestige was reduced .Wang Mingke gives another interpretation on this phenom-enon by using functionalism in his book titled the Qiang between the Han and Tibetan. 5 ) Investigating and sorting out religious scriptures The most prominent research on Qiang reli-gion during this period was made by Qian Anjing from Sichuan University .Based on the foundation built by Hu Jianming , Qian restarted collecting and sorting out the Qiang ’ s primitive religious scriptures and rituals, and, in doing so, he made a lot of contributions in this field .

  14. [Characteristics of non-marital and non-commercial heterosexual transmission of HIV infection in Miao-Dong Autonomous prefecture of Qiandongnan].

    Science.gov (United States)

    Yu, Q Y; Wang, F L; Xu, P; Wen, H J; Xiong, Y X; Yang, J; Long, Y; He, H J; Shi, J; Lyu, P

    2017-11-06

    Objective: The goal of this research was to understand the demographic distribution and related factors of non-marital and non-commercial heterosexual transmission (non-commercial transmission) for HIV/AIDS (human immunodeficiency virus/acquired immunodeficiency syndrome). Methods: Data related to HIV/AIDS infected by non-marital heterosexual transmission and whose present address was in Qian Dongnan, were collected from Information System on the HIV/AIDS Prevention and Control. Information included demographic characteristics, the members of non-marital sex partners, transmission path, detection source, CD4(+)T lymphocyte level, et al. cases belong to homosexual history, injective drug use or non-classified non-marital heterosexuality transmission were excluded, totally collect HIV/AIDS 919 cases. Multivariate logistic regressions were used to analyze potential factors associated with non-marital and non-commercial heterosexual transmission. In addition, in March and June 2017, using a convenience sampling, we conducted one-to-one interviews among 10 HIV/AIDS who were infected by non-marital heterosexuality and had non-marital and non-commercial heterosexual experience in Kaili Center for Disease Control and Prevention. The content of the interview included basic information, sexual orientation, the main place of making friends and sexual behavior, attitude to commercial heterosexuality and non-martial and non-commercial heterosexuality and so on. Results: Out of the 919 cases, 645 (70.2%) were male, the proportion of non-commercial transmission was 55.06% (506). The proportion of female HIV/AIDS with non-commercial transmission was 84.7% (232), which was higher than male (42.5%(274)) (χ(2)=138.35, Pcommercial transmission was 61.5% (275), which was higher than other religion (52.2%(412)) (χ(2)=6.32, P= 0.012). The proportion of HIV/AIDS with non-commercial transmission who had 0-5 non-marital sexual partners was 58.8% (498), which was higher than who had>5 non

  15. Capsaicin-loaded nanolipoidal carriers for topical application: design, characterization, and in vitro/in vivo evaluation

    Directory of Open Access Journals (Sweden)

    Wang XR

    2017-05-01

    Full Text Available Xia-Rong Wang,1 Si-Qian Gao,1 Xiao-Qian Niu,1 Long-Jian Li,2 Xiao-Ying Ying,1 Zhong-Jie Hu,2 Jian-Qing Gao1,3 1Institute of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, 2Zhejiang Provincial Corps Hospital of Chinese People’s Armed Police Forces, Jiaxing, Zhejiang, 3Jiangsu Engineering Research Center for New-Type External and Transdermal Preparations, Jiangsu, People’s Republic of China Abstract: Capsaicin has been used in clinical applications for the treatment of pain disorders and inflammatory diseases. Given the strong pungency and high oil/water partition coefficient of capsaicin, capsaicin-loaded nanolipoidal carriers (NLCs were designed to increase permeation and achieve the analgesic, anti-inflammatory effect with lower skin irritation. Capsaicin-loaded NLCs were prepared and later optimized by the Box–Behnken design. The physicochemical characterizations, morphology, and encapsulation of the capsaicin-loaded NLCs were subsequently confirmed. Capsaicin-loaded NLCs and capsaicin-loaded NLCs gel exhibited sustained release and no cytotoxicity properties. Also, they could significantly enhance the penetration amount, permeation flux, and skin retention amounts of capsaicin due to the application of NLCs. To study the topical permeation mechanism of capsaicin, 3,3'-dioctadecyloxacarbocyanine perchlorate (Dio was used as a fluorescent dye. Dio-loaded NLCs and Dio-loaded NLCs gel could effectively deliver Dio up to a skin depth of 260 and 210 µm, respectively, primarily through the appendage route on the basis of version skin sections compared with Dio solution, which only delivered Dio up to 150 µm. In vivo therapeutic experiments demonstrated that capsaicin-loaded NLCs and capsaicin-loaded NLCs gel could improve the pain threshold in a dose-dependent manner and inhibit inflammation, primarily by reducing the prostaglandin E2 levels in the tissue compared with capsaicin cream and capsaicin solution

  16. Implementation of an object oriented track reconstruction model into multiple LHC experiments*

    Science.gov (United States)

    Gaines, Irwin; Gonzalez, Saul; Qian, Sijin

    2001-10-01

    An Object Oriented (OO) model (Gaines et al., 1996; 1997; Gaines and Qian, 1998; 1999) for track reconstruction by the Kalman filtering method has been designed for high energy physics experiments at high luminosity hadron colliders. The model has been coded in the C++ programming language and has been successfully implemented into the OO computing environments of both the CMS (1994) and ATLAS (1994) experiments at the future Large Hadron Collider (LHC) at CERN. We shall report: how the OO model was adapted, with largely the same code, to different scenarios and serves the different reconstruction aims in different experiments (i.e. the level-2 trigger software for ATLAS and the offline software for CMS); how the OO model has been incorporated into different OO environments with a similar integration structure (demonstrating the ease of re-use of OO program); what are the OO model's performance, including execution time, memory usage, track finding efficiency and ghost rate, etc.; and additional physics performance based on use of the OO tracking model. We shall also mention the experience and lessons learned from the implementation of the OO model into the general OO software framework of the experiments. In summary, our practice shows that the OO technology really makes the software development and the integration issues straightforward and convenient; this may be particularly beneficial for the general non-computer-professional physicists.

  17. Distribution of Total and Organic Mercury in Superficial Soils in the Upper Manzanares River Watershed, Sucre State, Venezuela

    Directory of Open Access Journals (Sweden)

    Yahsé Rojas Challa

    2008-01-01

    Full Text Available Total and organic mercury contents were determined from samples of surface soils (0-5 cm, sieved at ≤ 63µm, collected from 10 different locations in the upper Manzanares River watershed, using cold vapor atomic absorption spectroscopy. Methylmercury was determined using a HPLC-UV detector. The mean total mercury concentration was 1.3 μg.g-1, a value permitted by the Canadian environment quality guidelines for farming soils, but high for European standards. Using certified reference materials, we verified that a modification of the method described by Qian et al. (2000 was effective for organic mercury extraction, with a recovery of 92.17% for DORM-2 and 92.11% for TORT-2. This modified method was applied to soil samples, obtaining concentrations of 0.5-1.0 μg.g-1 of organic mercury. The parameters for determining methylmercury using HPLC-UV were optimized; the best results were obtained with a 4.6 mm x 25 cm Zorbax CN column, with a mobile phase of 70/30 V/V of methanol: ammonium acetate 0.05 mol.l-1, with a flow rate of 0.5 ml.min-1; the methylmercury was detected at 4.99 min retention time. Methylmercury was not found in the soil samples. Using the certified reference material we proved that the method used produced reliable results. The analysis confirmed the existence of mercury in this farming area.

  18. Seroprevalence of Measles Antibodies and Predictors for Seropositivity among Chinese Children.

    Science.gov (United States)

    Wang, Xiaoqin; Ma, Mei; Hui, Zhaozhao; Terry, Paul D; Zhang, Yue; Su, Rui; Wang, Mingxu; Gu, Wei; Li, Ling

    2017-06-06

    Background : Supplementary measles immunization has been implemented since 2010 throughout China, yet few studies have reported its effect in the northwest regions. Methods: A cross-sectional study was conducted among children aged 2 to 4 years old ( n = 755) from February to September 2014 in 25 towns of Qian County, Shaanxi Province. Blood samples were analyzed for measles antibodies using enzyme-linked immunosorbent immunoglobulin G (IgG) assays. Socio-demographic factors were assessed by questionnaire. Data on vaccine dose were collected from town medical records. Univariate and logistic regression analyses were used to determine factors associated with measles antibody seropositivity. Results : Measles antibody seroprevalence was 91.13% (95% CI: 89.52-92.83) in our sample. Compared with children whose mother's highest education was primary school, seroprevalence was higher in children whose maternal education was middle school (adjusted OR: 1.4, 95% CI: 0.7-2.8), high school (adjusted OR: 2.4, 95% CI: 1.3-7.7), and college/university (adjusted OR: 2.9, 95% CI: 1.2-9.3). Vaccine dose was positively associated with seropositivity. Conclusions : Measles seroprevalence is high in China and is associated with the mother's education and vaccine dose.

  19. Lung cancer with concurrent EGFR mutation and ROS1 rearrangement: a case report and review of the literature

    Directory of Open Access Journals (Sweden)

    Zhu YC

    2016-07-01

    Full Text Available You-cai Zhu,1,2,* Chun-wei Xu,3,* Xiao-qian Ye,4 Man-xiang Yin,4 Jin-xian Zhang,2 Kai-qi Du,2 Zhi-hao Zhang,2 Jian Hu1 1Department of Thoracic Surgery, The First Affiliated Hospital of Medical School of Zhejiang University, Hangzhou, 2Department of Thoracic Surgery, Chinese People’s Armed Police Force, Zhejiang Corps Hospital, Jiaxing, Zhejiang, 3Department of Pathology, Affiliated Hospital of Academy of Military Medical Sciences, Beijing, 4Department of Pathology, Chinese People’s Armed Police Force, Zhejiang Corps Hospital, Jiaxing, Zhejiang, People’s Republic of China *These authors contributed equally to this work Abstract: ROS1 rearrangement has recently emerged as a new molecular subtype in non-small cell lung cancer, and is predominantly found in lung adenocarcinomas compared with other oncogenes such as EGFR, KRAS, or ALK. Patients who have both mutations are extremely rare. Here we report a 50-year-old female diagnosed with adenocarcinoma with sarcomatoid differentiation, who was shown to have EGFR and ROS1 mutations. The patient was treated surgically and received three cycles of adjuvant postoperative chemotherapy. In addition, we reviewed the previously reported cases and related literature. This presentation will provide further understanding of the underlying molecular biology and optimal treatment for non-small cell lung cancer patients with more than one driver mutation. Keywords: non-small cell lung cancer, EGFR gene mutation, ROS1 fusion gene

  20. CERN honours its guides

    CERN Multimedia

    2004-01-01

    At the end of January, CERN's guides were rewarded for their devotion to the Laboratory. They have a passion for their work, know CERN inside-out and for 40 years have shown people of all ages and nationalities, from all walks of life, around the Laboratory. Who are they? Why, the CERN guides, of course. On 27 January, ten of CERN's 180 guides received special honours for their impressive number of guided tours in 2003. Presenting the awards in the Microcosm hall, CERN's Director-General Robert Aymar congratulated the winners on the key role they play with respect to the general public. "CERN would be nothing without you who show them its activities," he stressed. CERN's Director-General Robert Aymar congratulates Alberto Ribon for his tally of over 40 visits in the course of 2003.One of the prizes was the book «The Particle Odyssey». Here the book's co-author Christine Sutton dedicates it for Sijin Qian. Tzanko Spassoff (PH) and retired staff members Klaus Batzner and Antonio Francano wo...

  1. Environmental impact analysis of mine tailing reservoir

    Science.gov (United States)

    Gong, J. Z.

    2016-08-01

    Under certain conditions landscape topography which utilizes mine tailing reservoir construction using is likely to increase lateral recharge source regions, resulting in dramatic changes to the local hydrological dynamic field and recharge of downstream areas initiated by runoff, excretion state, elevated groundwater depth, shallow groundwater, rainfall direct communication, and thinning of the vadose zone. Corrosive leaching of topsoil over many years of exposure to chemical fertilizers and pesticides may result in their dissolution into the groundwater system, which may lead to excessive amounts of many harmful chemicals, therby affecting the physical and mental health of human residents and increase environmental vulnerability and risk associated with the water and soil. According to field survey data from Yujiakan, Qian'an City, and Hebei provinces, this paper analyzes the hydrogeological environmental mechanisms of areas adjacent to mine tailing reservoirs and establishes a conceptual model of the local groundwater system and the concentration-response function between NO3 - content in groundwater and the incidence of cancer in local residents.

  2. Radiation effects on the immiscible polymer blend of nylon1010 and high-impact polystyrene (HIPS) I: Gel/dose curves, mathematical expectation theorem and thermal behaviour

    International Nuclear Information System (INIS)

    Dong, W.; Zhang, W.; Chen, G.; Liu, J.

    2000-01-01

    This paper studies the radiation properties of the immiscible blend of nylon1010 and HIPS. The gel fraction increased with increasing radiation dose. The network was found mostly in nylon1010, the networks were also found in both nylon1010 and HIPS when the dose reaches 0.85 MGy or more. We used the equation and the modified Zhang-Sun-Qian equation to simulate the relationship with the dose and the sol fraction. The latter equation fits well with these polymer blends and the relationship used by it showed better linearity than the one by the equation. We also studied the conditions of formation of the network by the mathematical expectation theorem for the binary system. Thermal properties of polymer blend were observed by DSC curves. The crystallization temperature decreases with increasing dose because the cross-linking reaction inhibited the crystallization procession and destroyed the crystals. The melting temperature also reduced with increasing radiation dose. The dual melting peak gradually shifted to single peak and the high melting peak disappeared at high radiation dose. However, the radiation-induced crystallization was observed by the heat of fusion increasing at low radiation dose. On the other hand, the crystal will be damaged by radiation. A similar conclusion may be drawn by the DSC traces when the polymer blends were crystallized. When the radiation dose increases, the heat of fusion reduces dramatically and so does the heat of crystallization. (author)

  3. Spectral Envelopes and Additive + Residual Analysis/Synthesis

    Science.gov (United States)

    Rodet, Xavier; Schwarz, Diemo

    The subject of this chapter is the estimation, representation, modification, and use of spectral envelopes in the context of sinusoidal-additive-plus-residual analysis/synthesis. A spectral envelope is an amplitude-vs-frequency function, which may be obtained from the envelope of a short-time spectrum (Rodet et al., 1987; Schwarz, 1998). [Precise definitions of such an envelope and short-time spectrum (STS) are given in Section 2.] The additive-plus-residual analysis/synthesis method is based on a representation of signals in terms of a sum of time-varying sinusoids and of a non-sinusoidal residual signal [e.g., see Serra (1989), Laroche et al. (1993), McAulay and Quatieri (1995), and Ding and Qian (1997)]. Many musical sound signals may be described as a combination of a nearly periodic waveform and colored noise. The nearly periodic part of the signal can be viewed as a sum of sinusoidal components, called partials, with time-varying frequency and amplitude. Such sinusoidal components are easily observed on a spectral analysis display (Fig. 5.1) as obtained, for instance, from a discrete Fourier transform.

  4. Laser Theory for Optomechanics: Limit Cycles in the Quantum Regime

    Directory of Open Access Journals (Sweden)

    Niels Lörch

    2014-01-01

    Full Text Available Optomechanical systems can exhibit self-sustained limit cycles where the quantum state of the mechanical resonator possesses nonclassical characteristics such as a strongly negative Wigner density, as was shown recently in a numerical study by Qian et al. [Phys. Rev. Lett. 109, 253601 (2012]. Here, we derive a Fokker-Planck equation describing mechanical limit cycles in the quantum regime that correctly reproduces the numerically observed nonclassical features. The derivation starts from the standard optomechanical master equation and is based on techniques borrowed from the laser theory due to Haake and Lewenstein. We compare our analytical model with numerical solutions of the master equation based on Monte Carlo simulations and find very good agreement over a wide and so far unexplored regime of system parameters. As one main conclusion, we predict negative Wigner functions to be observable even for surprisingly classical parameters, i.e., outside the single-photon strong-coupling regime, for strong cavity drive and rather large limit-cycle amplitudes. The approach taken here provides a natural starting point for further studies of quantum effects in optomechanics.

  5. Stepwise encapsulation and controlled two-stage release system for cis-Diamminediiodoplatinum

    Directory of Open Access Journals (Sweden)

    Chen Y

    2014-06-01

    Full Text Available Yun Chen,1,* Qian Li,1,2,* Qingsheng Wu1 1Department of Chemistry, Key Laboratory of Yangtze River Water Environment, Ministry of Education, Tongji University, Shanghai; 2Shanghai Institute of Quality Inspection and Technical Research, Shanghai, People’s Republic of China *These authors contributed equally to this work Abstract: cis-Diamminediiodoplatinum (cis-DIDP is a cisplatin-like anticancer drug with higher anticancer activity, but lower stability and price than cisplatin. In this study, a cis-DIDP carrier system based on micro-sized stearic acid was prepared by an emulsion solvent evaporation method. The maximum drug loading capacity of cis-DIDP-loaded solid lipid nanoparticles was 22.03%, and their encapsulation efficiency was 97.24%. In vitro drug release in phosphate-buffered saline (pH =7.4 at 37.5°C exhibited a unique two-stage process, which could prove beneficial for patients with tumors and malignancies. MTT (3-[4,5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide assay results showed that cis-DIDP released from cis-DIDP-loaded solid lipid nanoparticles had better inhibition activity than cis-DIDP that had not been loaded. Keywords: stearic acid, emulsion solvent evaporation method, drug delivery, cis-DIDP, in vitro

  6. Targeted magnetic iron oxide nanoparticles for tumor imaging and therapy

    Directory of Open Access Journals (Sweden)

    Xiang-Hong Peng

    2008-10-01

    Full Text Available Xiang-Hong Peng1,4, Ximei Qian2,4, Hui Mao3,4, Andrew Y Wang5, Zhuo (Georgia Chen1,4, Shuming Nie2,4, Dong M Shin1,4*1Department of Medical Oncology/Hematology; 2Department of Biomedical Engineering; 3Department of Radiology; 4Winship Cancer Institute, Emory University School of Medicine, Atlanta, GA, USA; 5Ocean Nanotech, LLC, Fayetteville, AR, USAAbstract: Magnetic iron oxide (IO nanoparticles with a long blood retention time, biodegradability and low toxicity have emerged as one of the primary nanomaterials for biomedical applications in vitro and in vivo. IO nanoparticles have a large surface area and can be engineered to provide a large number of functional groups for cross-linking to tumor-targeting ligands such as monoclonal antibodies, peptides, or small molecules for diagnostic imaging or delivery of therapeutic agents. IO nanoparticles possess unique paramagnetic properties, which generate significant susceptibility effects resulting in strong T2 and T*2 contrast, as well as T1 effects at very low concentrations for magnetic resonance imaging (MRI, which is widely used for clinical oncology imaging. We review recent advances in the development of targeted IO nanoparticles for tumor imaging and therapy.Keywords: iron oxide nanoparticles, tumor imaging, MRI, therapy

  7. Diversity and distribution of eukaryotic microbes in and around a brine pool adjacent to the Thuwal cold seeps in the Red Sea

    KAUST Repository

    Wang, Yong

    2014-02-04

    A hypoxic/suboxic brine pool at a depth of about 850 m was discovered near the Thuwal cold seeps in the Red Sea. Filled with high concentrations of hydrogen sulfide and ammonia, such a brine pool might limit the spread of eukaryotic organisms. Here, we compared the communities of the eukaryotic microbes in a microbial mat, sediments and water samples distributed in 7 sites within and adjacent to the brine pool. Taxonomic classification of the pyrosequenced 18S rRNA amplicon reads showed that fungi highly similar to the species identified along the Arabic coast were almost ubiquitous in the water and sediment samples, supporting their wide distribution in various environments. The microbial mat displayed the highest species diversity and contained grazers and a considerable percentage of unclassified species. Phylogeny-based methods revealed novel lineages representing a majority of the reads from the interface between the sea water and brine pool. Phylogenetic relationships with more reference sequences suggest that the lineages were affiliated with novel Alveolata and Euglenozoa inhabiting the interface where chemosynthetic prokaryotes are highly proliferative due to the strong chemocline and halocline. The brine sediments harbored abundant species highly similar to invertebrate gregarine parasites identified in different oxygen-depleted sediments. Therefore, the present findings support the uniqueness of some microbial eukaryotic groups in this cold seep brine system. 2014 Wang, Zhang, Cao, Shek, Tian, Wong, Batang, Al-suwailem and Qian.

  8. Orbital Period Variations in the NY Vir System, Revisited in the Light of New Data

    Directory of Open Access Journals (Sweden)

    Baştürk Özgür

    2018-02-01

    Full Text Available NY Virginis is an eclipsing binary system with a subdwarf B primary and an M type dwarf secondary. Recent studies (Qian et al. 2012; Lee et al. 2014 suggested the presence of two circumbinary planets with a few Jovian masses within the system. Lee et al. (2014 examined the orbital stabilities of the suggested planets, using the best-fit parameters derived from their eclipse timing variation analysis. They found that the outer companion should be ejected from the system in about 800 000 years. An observational report from Pulley et al. (2016 pointed out that the recent mideclipse times of the binary deviate significantly from the models suggested by Lee et al. (2014. In fact, variations in the orbital period of the system had already been recognized by many authors, but the parameters of these variations vary significantly as new data accumulate. Here, we analyze the eclipse timing variations of the NY Vir system, using new mid-eclipse times that we have obtained together with earlier published measurements in order to understand the nature of the system and constrain its parameters.

  9. Boosting ATM activity alleviates aging and extends lifespan in a mouse model of progeria.

    Science.gov (United States)

    Qian, Minxian; Liu, Zuojun; Peng, Linyuan; Tang, Xiaolong; Meng, Fanbiao; Ao, Ying; Zhou, Mingyan; Wang, Ming; Cao, Xinyue; Qin, Baoming; Wang, Zimei; Zhou, Zhongjun; Wang, Guangming; Gao, Zhengliang; Xu, Jun; Liu, Baohua

    2018-05-02

    DNA damage accumulates with age (Lombard et al., 2005). However, whether and how robust DNA repair machinery promotes longevity is elusive. Here, we demonstrate that ATM-centered DNA damage response (DDR) progressively declines with senescence and age, while low dose of chloroquine (CQ) activates ATM, promotes DNA damage clearance, rescues age-related metabolic shift, and prolongs replicative lifespan. Molecularly, ATM phosphorylates SIRT6 deacetylase and thus prevents MDM2-mediated ubiquitination and proteasomal degradation. Extra copies of Sirt6 extend lifespan in Atm-/- mice, with restored metabolic homeostasis. Moreover, the treatment with CQ remarkably extends lifespan of Caenorhabditis elegans , but not the ATM-1 mutants. In a progeria mouse model with low DNA repair capacity, long-term administration of CQ ameliorates premature aging features and extends lifespan. Thus, our data highlights a pro-longevity role of ATM, for the first time establishing direct causal links between robust DNA repair machinery and longevity, and providing therapeutic strategy for progeria and age-related metabolic diseases. © 2018, Qian et al.

  10. A hybrid parallel architecture for electrostatic interactions in the simulation of dissipative particle dynamics

    Science.gov (United States)

    Yang, Sheng-Chun; Lu, Zhong-Yuan; Qian, Hu-Jun; Wang, Yong-Lei; Han, Jie-Ping

    2017-11-01

    , which approximately take up most of the total simulation time. Although the parallel method CU-ENUF (Yang et al., 2016) based on GPU has achieved a qualitative leap compared with previous methods in electrostatic interactions computation, the computation capability is limited to the throughput capacity of a single GPU for super-scale simulation system. Therefore, we should look for an effective method to handle the calculation of electrostatic interactions efficiently for a simulation system with super-scale size. Solution method: We constructed a hybrid parallel architecture, in which CPU and GPU are combined to accelerate the electrostatic computation effectively. Firstly, the simulation system is divided into many subtasks via domain-decomposition method. Then MPI (Message Passing Interface) is used to implement the CPU-parallel computation with each computer node corresponding to a particular subtask, and furthermore each subtask in one computer node will be executed in GPU in parallel efficiently. In this hybrid parallel method, the most critical technical problem is how to parallelize a CUNFFT (nonequispaced fast Fourier transform based on CUDA) in the parallel strategy, which is conquered effectively by deep-seated research of basic principles and some algorithm skills. Restrictions: The HP-ENUF is mainly oriented to super-scale system simulations, in which the performance superiority is shown adequately. However, for a small simulation system containing less than 106 particles, the mode of multiple computer nodes has no apparent efficiency advantage or even lower efficiency due to the serious network delay among computer nodes, than the mode of single computer node. References: (1) S.-C. Yang, H.-J. Qian, Z.-Y. Lu, Appl. Comput. Harmon. Anal. 2016, http://dx.doi.org/10.1016/j.acha.2016.04.009. (2) S.-C. Yang, Y.-L. Wang, G.-S. Jiao, H.-J. Qian, Z.-Y. Lu, J. Comput. Chem. 37 (2016) 378. (3) S.-C. Yang, Y.-L. Zhu, H.-J. Qian, Z.-Y. Lu, Appl. Chem. Res. Chin. Univ

  11. Adenocarcinoma of the lung with EGFR gene mutation and subsequent resistance mechanisms exploration: case report

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    Xu L

    2017-09-01

    Full Text Available Li Xu,1,2 Qian Z Wang,1,2 Lin Wu1,2 1Department of the Second Chest Medicine, Hunan Cancer Hospital, Changsha, Hunan, People’s Republic of China; 2Department of the Second Chest Medicine, The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, Hunan, People’s Republic of China Abstract: The treatment of lung cancer has made paradigm-shift advancements in the past decade with the development of therapies directed at specific genetic alterations, such as epidermal growth factor receptor (EGFR. Here, we present a rare case of lung adenocarcinoma harboring EGFR activating mutation and ALK overexpression. During the EGFR-tyrosine kinase inhibitors treatment, next-generation sequencing revealed phosphatidylinositol 3-kinase/Akt/mammalian target of rapamycin pathway amplifications in tumor specimen and subsequent T790M mutation via plasma circulating tumor DNA. In conclusion, this case illustrates the existence of concomitant resistance mechanisms and demonstrates that circulating tumor DNA can reflect tumor heterogeneity. Keywords: epidermal growth factor receptor, PI3K/Akt/mTOR pathway, T790M, next-generation sequencing, circulating tumor DNA

  12. Seroprevalence of Measles Antibodies and Predictors for Seropositivity among Chinese Children

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    Xiaoqin Wang

    2017-06-01

    Full Text Available Background: Supplementary measles immunization has been implemented since 2010 throughout China, yet few studies have reported its effect in the northwest regions. Methods: A cross-sectional study was conducted among children aged 2 to 4 years old (n = 755 from February to September 2014 in 25 towns of Qian County, Shaanxi Province. Blood samples were analyzed for measles antibodies using enzyme-linked immunosorbent immunoglobulin G (IgG assays. Socio-demographic factors were assessed by questionnaire. Data on vaccine dose were collected from town medical records. Univariate and logistic regression analyses were used to determine factors associated with measles antibody seropositivity. Results: Measles antibody seroprevalence was 91.13% (95% CI: 89.52–92.83 in our sample. Compared with children whose mother’s highest education was primary school, seroprevalence was higher in children whose maternal education was middle school (adjusted OR: 1.4, 95% CI: 0.7–2.8, high school (adjusted OR: 2.4, 95% CI: 1.3–7.7, and college/university (adjusted OR: 2.9, 95% CI: 1.2–9.3. Vaccine dose was positively associated with seropositivity. Conclusions: Measles seroprevalence is high in China and is associated with the mother’s education and vaccine dose.

  13. Indirubin inhibits cell proliferation, migration, invasion and angiogenesis in tumor-derived endothelial cells

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    Li Z

    2018-05-01

    Full Text Available Zhuohong Li, Chaofu Zhu, Baiping An, Yu Chen, Xiuyun He, Lin Qian, Lan Lan, Shijie Li Department of Oncology, The Affiliated Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, China Purpose: Hepatocellular carcinoma is one of the most predominant malignancies with high fatality rate and its incidence is rising at an alarming rate because of its resistance to radio- and chemotherapy. Indirubin is the major active anti-tumor ingredient of a traditional Chinese herbal medicine. The present study aimed to analyze the effects of indirubin on cell proliferation, migration, invasion, and angiogenesis of tumor-derived endothelial cells (Td-EC. Methods: Td-EC were derived from human umbilical vein endothelial cells (HUVEC by treating HUVEC with the conditioned medium of human liver cancer cell line HepG2. Cell proliferation, migration, invasion, and angiogenesis were assessed by MTT, wound healing, in vitro cell invasion, and in vitro tube formation assay. Results: Td-EC were successfully obtained from HUVEC cultured with 50% culture supernatant from serum-starved HepG2 cells. Indirubin significantly inhibited Td-EC proliferation in a dose- and time-dependent manner. Indirubin also inhibited Td-EC migration, invasion, and angiogenesis. However, indirubin’s effects were weaker on HUVEC than Td-EC. Conclusion: Indirubin significantly inhibited Td-EC proliferation, migration, invasion, and angiogenesis. Keywords: indirubin, Td-EC, proliferation, migration, invasion, angiogenesis

  14. Environmental Applications of Nanotechnology

    Science.gov (United States)

    Keller, Arturo A.

    2014-07-01

    Engineered nanomaterials (ENMs) are currently used in many applications including agriculture (Gruère, 2012; Khot et al. 2012; Lopez-Moreno et al. 2010; Peralta-Videa et al. 2011; Zhao et al. 2012), aerogels (Bigall et al. 2009), aerospace (Baur and Silverman, 2007), automotive (Coelho et al. 2012; Presting and König, 2003; Salonitis et al. 2010), catalysts (Zhou et al. 2011), coatings, paints and pigments (Dhoke et al. 2009; Gopalakrishnan et al. 2011; Khanna, 2008), composites (Borchardt, 2003; Khanna and Bakshi, 2009; Petrov and Georgiev, 2012; Sahoo et al. 2010), construction (Lee et al. 2010), cosmetics (Musee, 2011; Sabitha et al. 2012; Singh and Nanda, 2012), electronics and optics (Alda et al. 2005; Avasthi et al. 2007; Song et al. 2012; Subramanian and Takhee, 2012), energy (Serrano et al. 2009), environmental remediation (Dionysiou 2004; Khin et al. 2012), filtration and purification (Dhakras, 2011; Savage and Diallo, 2005), food products (Blasco and Picó, 2011; Weiss et al. 2006), medical (Boisseau and Loubaton, 2011; Farokhzad and Langer, 2006), packaging (Silvestre et al. 2011), paper and board (Kharisov and Kharissova, 2010), plastics, security (Marín and Merkoçi, 2012), sensors (Ding et al. 2010; Duncan et al. 2012; Su et al. 2012; Tan et al. 2012), and textiles (Qian and Hinestroza, 2004; Wong et al. 2006), and research is underway on many new applications...

  15. [A study of the sexual art of having intercourse with several young virgins in traditional Chinese medicine].

    Science.gov (United States)

    Yan, Shanzhao

    2002-06-01

    The emergency treatments for the damage and bleeding of the vagina, and the sharp pubic pain of young virgins which were caused occurred by the sexual art, were recorded in traditional medicine books, such as the "Ji yan fang" and others. It is a fact that in ancient China some people used the sexual art of having intercourse with several young virgins at the same time in order to increase their health and keep perpetual youth and longevity. The famous traditional general-medical book, "qian jin yao fang" recommended that method to rich persons too. It is supposed that the beginning of the sexual art of having intercourse with several young virgins traces back to the times of Emperor Hanwu , but it seems to have disappeared from the historical stage in the Song period. On the other hand, the criticisms from the traditional medicine books and the secret languages of internal alchemy used for the Taoist sacred books show that the sexual art of having intercourse with several young virgins was still going on behind the scene in the Ming and Qing periods. Even if we consider the historical changes of ethics and mortality, we now cannot but criticize this behavior of abusing juveniles for the sexual art.

  16. 論戴震 (1723–1777 之考證哲學及其反思/On Dai Zhen’s (1723–1777 Philosophy of Evidential Research and Its Reflection

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    Jer-shiarn LEE

    2012-09-01

    Dai Zhen was known as a leading proponent of the evidential research (kaozheng school during the Qian Jia Era. However, he regarded kaozheng as primarily a means of revealing the truth. Moreover, he was regarded as a philosopher rather than as merely a kaozheng, or textual, scholar. In this respect, his philosophical writings reflect an attempt to substitute his own philosophy for the Song Philosophy of Principle (lixue. Dai Zhen’s philosophical writings indicate the impact philology had on philosophical issues. The methodology Dai Zhen adopted was essentially linguistic, that is, xungu (etymology, lit., “glossing”. The merit of this methodology was that it would trace back the original meanings of key terms in the Classics, and this in turn, would benefit the understanding of the Confucian tradition. The aim of this paper is to explore Dai Zhen’s attempt to reconstruct the Confucian tradition, and to assess his philosophy of evidential research in order to manifest its value and limitation.

  17. Image simulation and surface reconstruction of undercut features in atomic force microscopy

    Science.gov (United States)

    Qian, Xiaoping; Villarrubia, John; Tian, Fenglei; Dixson, Ronald

    2007-03-01

    CD-AFMs (critical dimension atomic force microscopes) are instruments with servo-control of the tip in more than one direction. With appropriately "boot-shaped" or flared tips, such instruments can image vertical or even undercut features. As with any AFM, the image is a dilation of the sample shape with the tip shape. Accurate extraction of the CD requires a correction for the tip effect. Analytical methods to correct images for the tip shape have been available for some time for the traditional (vertical feedback only) AFMs, but were until recently unavailable for instruments with multi-dimensional feedback. Dahlen et al. [J. Vac. Sci. Technol. B23, pp. 2297-2303, (2005)] recently introduced a swept-volume approach, implemented for 2-dimensional (2D) feedback. It permits image simulation and sample reconstruction, techniques previously developed for the traditional instruments, to be extended for the newer tools. We have introduced [X. Qian and J. S. Villarrubia, Ultramicroscopy, in press] an alternative dexel-based method, that does the same in either 2D or 3D. This paper describes the application of this method to sample shapes of interest in semiconductor manufacturing. When the tip shape is known (e.g., by prior measurement using a tip characterizer) a 3D sample surface may be reconstructed from its 3D image. Basing the CD measurement upon such a reconstruction is shown here to remove some measurement artifacts that are not removed (or are incompletely removed) by the existing measurement procedures.

  18. Conservative fragments in bacterial 16S rRNA genes and primer design for 16S ribosomal DNA amplicons in metagenomic studies

    KAUST Repository

    Wang, Yong

    2009-10-09

    Bacterial 16S ribosomal DNA (rDNA) amplicons have been widely used in the classification of uncultured bacteria inhabiting environmental niches. Primers targeting conservative regions of the rDNAs are used to generate amplicons of variant regions that are informative in taxonomic assignment. One problem is that the percentage coverage and application scope of the primers used in previous studies are largely unknown. In this study, conservative fragments of available rDNA sequences were first mined and then used to search for candidate primers within the fragments by measuring the coverage rate defined as the percentage of bacterial sequences containing the target. Thirty predicted primers with a high coverage rate (>90%) were identified, which were basically located in the same conservative regions as known primers in previous reports, whereas 30% of the known primers were associated with a coverage rate of <90%. The application scope of the primers was also examined by calculating the percentages of failed detections in bacterial phyla. Primers A519-539, E969- 983, E1063-1081, U515 and E517, are highly recommended because of their high coverage in almost all phyla. As expected, the three predominant phyla, Firmicutes, Gemmatimonadetes and Proteobacteria, are best covered by the predicted primers. The primers recommended in this report shall facilitate a comprehensive and reliable survey of bacterial diversity in metagenomic studies. © 2009 Wang, Qian.

  19. A simple theory of motor protein kinetics and energetics. II.

    Science.gov (United States)

    Qian, H

    2000-01-10

    A three-state stochastic model of motor protein [Qian, Biophys. Chem. 67 (1997) pp. 263-267] is further developed to illustrate the relationship between the external load on an individual motor protein in aqueous solution with various ATP concentrations and its steady-state velocity. A wide variety of dynamic motor behavior are obtained from this simple model. For the particular case of free-load translocation being the most unfavorable step within the hydrolysis cycle, the load-velocity curve is quasi-linear, V/Vmax = (cF/Fmax-c)/(1-c), in contrast to the hyperbolic relationship proposed by A.V. Hill for macroscopic muscle. Significant deviation from the linearity is expected when the velocity is less than 10% of its maximal (free-load) value--a situation under which the processivity of motor diminishes and experimental observations are less certain. We then investigate the dependence of load-velocity curve on ATP (ADP) concentration. It is shown that the free load Vmax exhibits a Michaelis-Menten like behavior, and the isometric Fmax increases linearly with ln([ATP]/[ADP]). However, the quasi-linear region is independent of the ATP concentration, yielding an apparently ATP-independent maximal force below the true isometric force. Finally, the heat production as a function of ATP concentration and external load are calculated. In simple terms and solved with elementary algebra, the present model provides an integrated picture of biochemical kinetics and mechanical energetics of motor proteins.

  20. In vitro production of huperzine A, a promising drug candidate for Alzheimer's disease.

    Science.gov (United States)

    Ma, Xiaoqiang; Gang, David R

    2008-07-01

    Alzheimer's disease (AD) is growing in impact on human health. With no known cure, AD is one of the most expensive diseases in the world to treat. Huperzine A (HupA), a anti-AD drug candidate from the traditional Chinese medicine Qian Ceng Ta (Huperzia serrata), has been shown to be a powerful and selective inhibitor of acetylcholinesterase and has attracted widespread attention because of its unique pharmacological activities and low toxicity. As a result, HupA is becoming an important lead compound for drugs to treat AD. HupA is obtained naturally from very limited and slowly growing natural resources, members of the Huperziaceae. Unfortunately, the content of HupA is very low in the raw plant material. This has led to strong interest in developing sources of HupA. We have developed a method to propagate in vitro tissues of Phlegmariurus squarrosus, a member of the Huperziaceae, that produce high levels of HupA. The in vitro propagated tissues produce even higher levels of HupA than the natural plant, and may represent an excellent source for HupA.

  1. Huperzine A: Is it an Effective Disease-Modifying Drug for Alzheimer's Disease?

    Science.gov (United States)

    Qian, Zhong Ming; Ke, Ya

    2014-01-01

    Alzheimer's disease (AD) is a progressive neurodegenerative disorder for which there is no cure. Huperzine A (HupA) is a natural inhibitor of acetylcholinesterase (AChE) derived from the Chinese folk medicine Huperzia serrata (Qian Ceng Ta). It is a licensed anti-AD drug in China and is available as a nutraceutical in the US. A growing body of evidence has demonstrated that HupA has multifaceted pharmacological effects. In addition to the symptomatic, cognitive-enhancing effect via inhibition of AChE, a number of recent studies have reported that this drug has "non-cholinergic" effects on AD. Most important among these is the protective effect of HupA on neurons against amyloid beta-induced oxidative injury and mitochondrial dysfunction as well as via the up-regulation of nerve growth factor and antagonizing N-methyl-d-aspartate receptors. The most recent discovery that HupA may reduce brain iron accumulation lends further support to the argument that HupA could serve as a potential disease-modifying agent for AD and also other neurodegenerative disorders by significantly slowing down the course of neuronal death.

  2. Huperzine A: Is it an Effective Disease-Modifying Drug for Alzheimer’s Disease?

    Science.gov (United States)

    Qian, Zhong Ming; Ke, Ya

    2014-01-01

    Alzheimer’s disease (AD) is a progressive neurodegenerative disorder for which there is no cure. Huperzine A (HupA) is a natural inhibitor of acetylcholinesterase (AChE) derived from the Chinese folk medicine Huperzia serrata (Qian Ceng Ta). It is a licensed anti-AD drug in China and is available as a nutraceutical in the US. A growing body of evidence has demonstrated that HupA has multifaceted pharmacological effects. In addition to the symptomatic, cognitive-enhancing effect via inhibition of AChE, a number of recent studies have reported that this drug has “non-cholinergic” effects on AD. Most important among these is the protective effect of HupA on neurons against amyloid beta-induced oxidative injury and mitochondrial dysfunction as well as via the up-regulation of nerve growth factor and antagonizing N-methyl-d-aspartate receptors. The most recent discovery that HupA may reduce brain iron accumulation lends further support to the argument that HupA could serve as a potential disease-modifying agent for AD and also other neurodegenerative disorders by significantly slowing down the course of neuronal death. PMID:25191267

  3. Huperzine A: is it an effective disease-modifying drug for Alzheimer’s disease?

    Directory of Open Access Journals (Sweden)

    Zhong Ming eQian

    2014-08-01

    Full Text Available Alzheimer's disease (AD is a progressive neurodegenerative disorder for which there is no cure. Huperzine A (HupA is a natural inhibitor of acetylcholinesterase (AChE derived from the Chinese folk medicine Huperzia serrata (Qian Ceng Ta. It is a licensed anti-AD drug in China and is available as a nutraceutical in the US. A growing body of evidence has demonstrated that HupA has multifaceted pharmacological effects. In addition to the symptomatic, cognitive-enhancing effect via inhibition of AChE, a number of recent studies have reported that this drug has non-cholinergic effects on AD. Most important among these is the protective effect of HupA on neurons against amyloid beta-induced oxidative injury and mitochondrial dysfunction as well as via the up-regulation of nerve growth factor and antagonizing N-methyl-D-aspartate receptors. The most recent discovery that HupA may reduce brain iron accumulation lends further support to the argument that HupA could serve as a potential disease-modifying agent for AD and also other neurodegenerative disorders by significantly slowing down the course of neuronal death.

  4. Elastocaloric cooling materials and systems

    Science.gov (United States)

    Takeuchi, Ichiro

    2015-03-01

    We are actively pursuing applications of thermoelastic (elastocaloric) cooling using shape memory alloys. Latent heat associated with martensitic transformation of shape memory alloys can be used to run cooling cycles with stress-inducing mechanical drives. The coefficient of performance of thermoelastic cooling materials can be as high as 11 with the directly measured DT of around 17 °C. Depending on the stress application mode, the number of cycles to fatigue can be as large as of the order of 105. Efforts to design and develop thermoelastic alloys with long fatigue life will be discussed. The current project at the University of Maryland is focused on development of building air-conditioners, and at Maryland Energy and Sensor Technologies, smaller scale commercial applications are being pursued. This work is carried out in collaboration with Jun Cui, Yiming Wu, Suxin Qian, Yunho Hwang, Jan Muehlbauer, and Reinhard Radermacher, and it is funded by the ARPA-E BEETIT program and the State of Maryland.

  5. Evidence of topological insulator state in the semimetal LaBi

    Science.gov (United States)

    Lou, R.; Fu, B.-B.; Xu, Q. N.; Guo, P.-J.; Kong, L.-Y.; Zeng, L.-K.; Ma, J.-Z.; Richard, P.; Fang, C.; Huang, Y.-B.; Sun, S.-S.; Wang, Q.; Wang, L.; Shi, Y.-G.; Lei, H. C.; Liu, K.; Weng, H. M.; Qian, T.; Ding, H.; Wang, S.-C.

    2017-03-01

    By employing angle-resolved photoemission spectroscopy combined with first-principles calculations, we performed a systematic investigation on the electronic structure of LaBi, which exhibits extremely large magnetoresistance (XMR), and is theoretically predicted to possess band anticrossing with nontrivial topological properties. Here, the observations of the Fermi-surface topology and band dispersions are similar to previous studies on LaSb [L.-K. Zeng, R. Lou, D.-S. Wu, Q. N. Xu, P.-J. Guo, L.-Y. Kong, Y.-G. Zhong, J.-Z. Ma, B.-B. Fu, P. Richard, P. Wang, G. T. Liu, L. Lu, Y.-B. Huang, C. Fang, S.-S. Sun, Q. Wang, L. Wang, Y.-G. Shi, H. M. Weng, H.-C. Lei, K. Liu, S.-C. Wang, T. Qian, J.-L. Luo, and H. Ding, Phys. Rev. Lett. 117, 127204 (2016), 10.1103/PhysRevLett.117.127204], a topologically trivial XMR semimetal, except the existence of a band inversion along the Γ -X direction, with one massless and one gapped Dirac-like surface state at the X and Γ points, respectively. The odd number of massless Dirac cones suggests that LaBi is analogous to the time-reversal Z2 nontrivial topological insulator. These findings open up a new series for exploring novel topological states and investigating their evolution from the perspective of topological phase transition within the family of rare-earth monopnictides.

  6. Determinations of its Absolute Dimensions and Distance by the Analyses of Light and Radial-Velocity Curves of the Contact Binary -I. V417 Aquilae

    Directory of Open Access Journals (Sweden)

    Jae Woo Lee

    2004-06-01

    Full Text Available New photometric and spectroscopic solutions of W-type overcontact binary V417 Aql were obtained by solving the UBV light curves of Samec et al. (1997 and radial-velocity ones of Lu & Rucinski (1999 with the 2003 version of the Wilson-Devinney binary code. In the light curve synthesis the light of a third-body, which Qian (2003 proposed, was considered and obtained about 2.7%, 2.2%, and 0.4% for U, B, and V bandpasses, respectively. The model with third-light is better fitted to eclipse parts than that with no third-light. Absolute dimensions of V417 Aql are determined from our solution as M1=0.53 M⊙, M2=1.45 M⊙, R1=0.84 R⊙ and R2=1.31 M⊙, and the distance to it is deduced as about 216pc. Our distance is well consistent with that (204pc derived from Rucinski & Duerbeck's (1997 relation, MV=MV(log P, B-V, but is more distant than that (131±40pc determined by the Hipparcos trigonometric parallax. The difference may result from the relatively large error of Hipparcos parallax for V417 Aql.

  7. Visual Search for Motion-Form Conjunctions: Selective Attention to Movement Direction.

    Science.gov (United States)

    Von Mühlenen, Adrian; Müller, Hermann J

    1999-07-01

    In 2 experiments requiring visual search for conjunctions of motion and form, the authors reinvestigated whether motion-based filtering (e.g., P. McLeod, J. Driver, Z. Dienes, & J. Crisp, 1991) is direction selective and whether cuing of the target direction promotes efficient search performance. In both experiments, the authors varied the number of movement directions in the display and the predictability of the target direction. Search was less efficient when items moved in multiple (2, 3, and 4) directions as compared with just 1 direction. Furthermore, precuing of the target direction facilitated the search, even with "wrap-around" displays, relatively more when items moved in multiple directions. The authors proposed 2 principles to explain that pattern of effects: (a) interference on direction computation between items moving in different directions (e.g., N. Qian & R. A. Andersen, 1994) and (b) selective direction tuning of motion detectors involving a receptive-field contraction (cf. J. Moran & R. Desimone, 1985; S. Treue & J. H. R. Maunsell, 1996).

  8. Relationship between tongue strength, lip strength, and nutrition-related sarcopenia in older rehabilitation inpatients: a cross-sectional study

    Directory of Open Access Journals (Sweden)

    Sakai K

    2017-08-01

    Full Text Available Kotomi Sakai,1,2 Enri Nakayama,2 Haruka Tohara,3 Keiji Kodama,4 Takahiro Takehisa,5 Yozo Takehisa,6 Koichiro Ueda2 1Department of Rehabilitation Medicine, Setagaya Memorial Hospital, 2Department of Dysphagia Rehabilitation, Nihon University School of Dentistry, 3Gerodontology and Oral Rehabilitation, Department of Gerontology and Gerodontology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 4Department of Internal Medicine, 5Department of Orthopaedic Surgery, Setagaya Memorial Hospital, Tokyo, 6Department of Internal Medicine, Hakuai Memorial Hospital, Tokushima, Japan Objective: The objective of this study was to clarify the relationship between tongue strength, lip strength, and nutrition-related sarcopenia (NRS.Patients and methods: A total of 201 older inpatients aged ≥65 years (70 men, median age: 84 years, interquartile range: 79–89 years consecutively admitted for rehabilitation were included in this cross-sectional study. The main factors evaluated were the presence of NRS diagnosed by malnutrition using the Mini-Nutrition Assessment – Short Form, sarcopenia based on the criteria of the Asian Working Group for Sarcopenia, tongue strength, and lip strength. Other factors such as age, sex, comorbidity, physical function, cognitive function, and oral intake level were also assessed.Results: In all, 78 (38.8% patients were allocated to the NRS group, and 123 (61.2% patients were allocated to the non-NRS group. The median tongue strength and lip strength (interquartile range were significantly lower in the NRS group (tongue: 22.9 kPa [17.7–27.7 kPa] and lip: 7.2 N [5.6–9.8 N] compared with the non-NRS group (tongue: 29.7 kPa [24.8–35.1 kPa] and lip: 9.9 N [8.4–12.3 N], P<0.001 for both. Multivariable logistic regression analysis showed that NRS was independently associated with tongue strength (odds ratio [OR] =0.93, 95% confidence interval [CI] 0.87–0.98, P=0.012 and lip

  9. Numerical Simulation of Floating Bodies in Extreme Free Surface Waves

    Science.gov (United States)

    Hu, Zheng Zheng; Causon, Derek; Mingham, Clive; Qiang, Ling

    2010-05-01

    and efficient. Firstly, extreme design wave conditions are generated in an empty NWT and compared with physical experiments as a precursor to calculations to investigate the survivability of the Bobber device operating in a challenging wave climate. Secondly, we consider a bench-mark test case involving in a first order regular wave maker acting on a fixed cylinder and Pelamis. Finally, a floating Bobber has been simulated under extreme wave conditions. These results will be reported at the meeting. Causon D.M., Ingram D.M., Mingham C.G., Yang G. Pearson R.V. (2000). Calculation of shallow water flows using a Cartesian cut cell approach. Advances in Water resources, 23: 545-562. Causon D.M., Ingram D.M., Mingham C.G. (2000). A Cartesian cut cell method for shallow water flows with moving boundaries. Advances in Water resources, 24: 899-911. Dalzell J.F. 1999 A note on finite depth second-order wave-wave interactions. Appl. Ocean Res. 21, 105-111. Ning D.Z., Zang J., Liu S.X. Eatock Taylor R. Teng B. & Taylor P.H. 2009 Free surface and wave kinematics for nonlinear focused wave groups. J. Ocean Engineering. Accepted. Hu Z.Z., Causon D.M., Mingham C.M. and Qian L.(2009). Numerical wave tank study of a wave energy converter in heave. Proceedlings 19th ISOPE conference, Osaka, Japan Qian L., Causon D.M. & Mingham C.G., Ingram D.M. 2006 A free-surface capturing method for two fluid flows with moving bodies. Proc. Roy. Soc. London, Vol. A 462 21-42.

  10. 气相中双液滴正碰过程数值模拟研究%Numerical simulation of head-on binary collision of droplets in gas phase environment

    Institute of Scientific and Technical Information of China (English)

    刘泽军; 张超; 武丽丽; 吴建军

    2015-01-01

    液滴碰撞现象普遍存在于动力装置燃烧室喷嘴的下游区域,影响燃料的雾化性能。为了揭示相同直径的双液滴中心碰撞机理,求解了轴对称坐标系下的 N-S 方程,采用 VOF(Volume of Fluid)方法捕捉液滴碰撞过程中气液自由表面的演化规律。利用 Qian 等提供的实验结果对计算模型进行数值校验,验证了模型的准确性。在此基础上,研究了环境压强对液滴碰撞反弹后不同结果(分离和融合)的影响,分析了环境压强和 Weber 数对液滴碰撞分离的影响。结果表明,液滴在碰撞反弹后的状态(分离或融合)是由液滴间气膜压强与环境气动阻力共同作用的结果,环境压强对液滴碰撞分离过程基本没有影响;Weber 数越大,碰撞过程中变形的幅度越大。%The phenomena of droplet collision not only exist widely in the downstream region of ejection nozzles in the combustion chambers of power equipments,but also they will affect the atomization characteristics of fuel.In order to reveal the binary collision mechanism of droplets with equal diameters, the Navier-Stokes equations are firstly solved in the axisymmetric coordinates and the method of VOF (Volume of Fluid)is utilized to track the evolution disciplines of the gas-liquid free surface during the process of droplet collision.Then,veracity of the numerical models is validated with the experimental results of Qian.Effects of ambient pressure on different results (separation and coalescence)after drop-let bouncing are investigated,and effects of ambient pressure and Weber number on separation of two droplets after collision are also analyzed.Results show that states (separation and coalescence)of drop-lets after collision and rebound are determined by the pressure of gaseous film between droplets and gas-eous dynamic resistance together.Moreover,the separation process of two droplets after collision is inde-pendent of the ambient pressure

  11. Anti-EGFR-iRGD recombinant protein conjugated silk fibroin nanoparticles for enhanced tumor targeting and antitumor efficiency

    Directory of Open Access Journals (Sweden)

    Bian X

    2016-05-01

    Full Text Available Xinyu Bian,* Puyuan Wu,* Huizi Sha, Hanqing Qian, Qing Wang, Lei Cheng, Yang Yang, Mi Yang, Baorui LiuComprehensive Cancer Center of Drum-Tower Hospital, Medical School of Nanjing University, Clinical Cancer Institute of Nanjing University, Nanjing, People’s Republic of China*These authors contributed equally to this workAbstract: In this study, we report a novel kind of targeting with paclitaxel (PTX-loaded silk fibroin nanoparticles conjugated with iRGD–EGFR nanobody recombinant protein (anti-EGFR-iRGD. The new nanoparticles (called A-PTX-SF-NPs were prepared using the carbodiimide-mediated coupling procedure and their characteristics were evaluated. The cellular cytotoxicity and cellular uptake of A-PTX-SF-NPs were also investigated. The results in vivo suggested that NPs conjugated with the recombinant protein exhibited more targeting and anti-neoplastic property in cells with high EGFR expression. In the in vivo antitumor efficacy assay, the A-PTX-SF-NPs group showed slower tumor growth and smaller tumor volumes than PTX-SF-NPs in a HeLa xenograft mouse model. A real-time near-infrared fluorescence imaging study showed that A-PTX-SF-NPs could target the tumor more effectively. These results suggest that the anticancer activity and tumor targeting of A-PTX-SF-NPs were superior to those of PTX-SF-NPs and may have the potential to be used for targeted delivery for tumor therapies. Keywords: EGFR, nanobody, iRGD, recombinant protein, targeting drug carriers, antitumor efficiency

  12. Enhanced photocytotoxicity of curcumin delivered by solid lipid nanoparticles

    Directory of Open Access Journals (Sweden)

    Jiang S

    2016-12-01

    Full Text Available Shan Jiang,1 Rongrong Zhu,1 Xiaolie He,1 Jiao Wang,1 Mei Wang,1 Yechang Qian,2 Shilong Wang1 1Tenth People’s Hospital, School of Life Science and Technology, Tongji University, 2Department of Respiratory Disease, Baoshan District Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai, People’s Republic of China Abstract: Curcumin (Cur is a promising photosensitizer that could be used in photodynamic therapy. However, its poor solubility and hydrolytic instability limit its clinical use. The aim of the present study was to encapsulate Cur into solid lipid nanoparticles (SLNs in order to improve its therapeutic activity. The Cur-loaded SLNs (Cur-SLNs were prepared using an emulsification and low-temperature solidification method. The functions of Cur and Cur-SLNs were studied on the non-small cell lung cancer A549 cells for photodynamic therapy. The results revealed that Cur-SLNs induced ~2.27-fold toxicity higher than free Cur at a low concentration of 15 µM under light excitation, stocking more cell cycle at G2/M phase. Cur-SLNs could act as an efficient drug delivery system to increase the intracellular concentration of Cur and its accumulation in mitochondria; meanwhile, the hydrolytic stability of free Cur could be improved. Furthermore, Cur-SLNs exposed to 430 nm light could produce more reactive oxygen species to induce the disruption of mitochondrial membrane potential. Western blot analysis revealed that Cur-SLNs increased the expression of caspase-3, caspase-9 proteins and promoted the ratio of Bax/Bcl-2. Overall, the results from these studies demonstrated that the SLNs could enhance the phototoxic effects of Cur. Keywords: photodynamic therapy, curcumin, solid lipid nanoparticles, drug delivery, reactive oxygen species

  13. FIRST PHOTOMETRIC INVESTIGATION OF THE NEWLY DISCOVERED W UMa-TYPE BINARY STAR MR Com

    Energy Technology Data Exchange (ETDEWEB)

    Qian, S.-B.; Liu, N.-P.; Liao, W.-P.; He, J.-J.; Liu, L.; Zhu, L.-Y.; Wang, J.-J.; Zhao, E.-G., E-mail: qsb@ynao.ac.cn [Yunnan Observatories, Chinese Academy of Sciences (CAS), P.O. Box 110, 650011 Kunming (China)

    2013-08-01

    By analyzing multi-color light curves of the newly discovered W UMa-type binary, MR Com, we discovered that it is a shallow-contact binary with a degree of contact factor of f = 10.0% {+-} 2.1%. Photometric solutions reveal that MR Com is a W-type system with a mass ratio of q = 3.9 where the less massive component is about 90 K hotter than the more massive one. By investigating all of the available times of minimum light, we found that the general trend of the Observed-Calculated (O - C) curve shows a downward parabolic variation while it undergoes a cyclic variation with a small amplitude of 0.0031 days and a period of 10.1 yr. The downward parabolic change corresponds to a long-term decrease in the orbital period at a rate of P-dot = -5.3 x 10{sup -7} days yr{sup -1} that may be caused by a combination of a mass transfer and an angular momentum loss (AML) via magnetic braking. Among the 16 shallow-contact systems with a decreasing orbital period, MR Com has the lowest mass ratio (e.g., 1/q = 0.26). The shallow-contact configuration, the low-mass ratio, and the long-term period decrease all suggest that systems similar to MR Com are on the AML-controlled stage of the evolutionary scheme proposed by Qian. They will oscillate around a critical mass ratio and evolve into a deep contact with a higher mass ratio. The small-amplitude cyclic change in the O - C curve was analyzed for the light-travel time effect via the presence of an extremely cool stellar companion.

  14. Facies-succession and architecture of the third-order sequences and their stratigraphic framework of the Devonian in Yunnan-Guizhou-Guangxi area, South China

    Directory of Open Access Journals (Sweden)

    Mei Mingxiang

    2013-01-01

    Full Text Available The Caledonian orogeny at the end of the Silurian resulted in great changes in the palaeogeography in the Yunnan-Guizhou-Guangxi area of South China; the continental area of the Early Paleozoic evolved into the extensive Dian-Qian-Gui Sea in the Late Paleozoic. Early in the Devonian, as a result of a major transgression, seawater encroached gradually from the south to the north and clastic facies were deposited. Carbonate deposition was then established in the Yunnan-Guizhou-Guangxi area, with a palaeogeography marked by attached platforms, isolated platforms and narrow basins. As a result of the Ziyun movement towards the end of the Devonian, the Upper Devonian strata are regressive and thin out from the open-sea to the land areas. A study of the nature and distribution of sedimentary facies in space and time recognises 13 third-order sequences in the Devonian strata in Yunnan-Guizhou-Guangxi area, and these form two second-order sequences. The strata of the Lower Devonian comprise 5 third-order sequences (SQ1 to SQ5, which are dominated by transgressive clastics. 4 third-order sequences (SQ6 to SQ9 in the Middle Devonian are characterized by alternations of transgressive clastics and highstand carbonates. In the Upper Devonian, carbonates constitute 4 third-order sequences (SQ10 to SQ13, which are generally marked by the transgressive limestones and highstand dolomites. On the basis of earlier biostratigraphic studies, sea-level changes represented by the third-order sequences with their different facies successions are explored, and the sequence stratigraphic framework is established. Therefore, the Devonian strata in the study area provide an example for further understanding of depositional trends within the sequence-stratigraphic framework.

  15. A Variational Model for Two-Phase Immiscible Electroosmotic Flow at Solid Surfaces

    KAUST Repository

    Shao, Sihong

    2012-01-01

    We develop a continuum hydrodynamic model for two-phase immiscible flows that involve electroosmotic effect in an electrolyte and moving contact line at solid surfaces. The model is derived through a variational approach based on the Onsager principle of minimum energy dissipation. This approach was first presented in the derivation of a continuum hydrodynamic model for moving contact line in neutral two-phase immiscible flows (Qian, Wang, and Sheng, J. Fluid Mech. 564, 333-360 (2006)). Physically, the electroosmotic effect can be formulated by the Onsager principle as well in the linear response regime. Therefore, the same variational approach is applied here to the derivation of the continuum hydrodynamic model for charged two-phase immiscible flows where one fluid component is an electrolyte exhibiting electroosmotic effect on a charged surface. A phase field is employed to model the diffuse interface between two immiscible fluid components, one being the electrolyte and the other a nonconductive fluid, both allowed to slip at solid surfaces. Our model consists of the incompressible Navier-Stokes equation for momentum transport, the Nernst-Planck equation for ion transport, the Cahn-Hilliard phase-field equation for interface motion, and the Poisson equation for electric potential, along with all the necessary boundary conditions. In particular, all the dynamic boundary conditions at solid surfaces, including the generalized Navier boundary condition for slip, are derived together with the equations of motion in the bulk region. Numerical examples in two-dimensional space, which involve overlapped electric double layer fields, have been presented to demonstrate the validity and applicability of the model, and a few salient features of the two-phase immiscible electroosmotic flows at solid surface. The wall slip in the vicinity of moving contact line and the Smoluchowski slip in the electric double layer are both investigated. © 2012 Global-Science Press.

  16. Photoresponsive nanocapsulation of cobra neurotoxin and enhancement of its central analgesic effects under red light

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    Yang Q

    2017-05-01

    Full Text Available Qian Yang, Chuang Zhao, Jun Zhao, Yong Ye Department of Pharmaceutical Engineering, School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, People’s Republic of China Abstract: Cobra neurotoxin (CNT, a peptide isolated from snake venom of Naja naja atra, shows central analgesic effects in our previous research. In order to help CNT pass through blood–brain barrier (BBB and improve its central analgesic effects, a new kind of CNT nanocapsules were prepared by double emulsification with soybean lecithin and cholesterol as the shell, and pheophorbide as the photosensitizer added to make it photoresponsive. The analgesic effects were evaluated by hot plate test and acetic acid-induced writhing in mice. The CNT nanocapsules had an average particle size of 229.55 nm, zeta potential of -53.00 mV, encapsulation efficiency of 84.81% and drug loading of 2.98%, when the pheophorbide content was 1% of lecithin weight. Pheophorbide was mainly distributed in outer layer of the CNT nanocapsules and increased the release of the CNT nanocapsules after 650 nm illumination. The central analgesic effects were improved after intraperitoneal injection of CNT at 25 and 50 µg·kg-1 under 650 nm irradiation for 30 min in the nasal cavity. Activation of pheophorbide by red light generated reactive oxygen species which opened the nanocapsules and BBB and helped the CNT enter the brain. This research provides a new drug delivery for treatment of central pain. Keywords: cobra neurotoxin, nanocapsules, photoresponsive, central analgesic effects, red light, drug delivery, photosensitizer

  17. Research of combination model for prediction of the trend of outbreak of hepatitis B

    Directory of Open Access Journals (Sweden)

    Yin-ping CHEN

    2014-03-01

    Full Text Available Objective To establish a combination model of autoregressive integrated moving average model and the grey dynamics (ARIMA-GM of hepatitis B incidence rate (1/100 000 to predict the trend of outbreak of hepatitis B, as to provide a scientific basis for the early discovery of the infectious diseases for the performance of countermeasures of controlling its spread. Methods The monthly incidence of hepatitis B in Qian'an city, Hebei province, was collected from Jan 2004 to Dec 2012, and a model (ARIMA was reproduced with SPSS software. The GM (1,1 model was used to correct the residual sequence with a threshold value, and a combined forecasting model was reproduced. This combination model was used to predict the monthly incidence rate in this city in 2013. Results The model ARIMA(0,1,1(0,1,112 was established successfully and the residual sequence was a white noise sequence. Then the GM (1,1 model with a threshold of 3 was used to correct its residuals and obtain its nonlinear feature extraction of information. The forecasting model met required precision standards (C=0.673, P=0.877, the fitting accuracy of which was basically qualified. The results showed that the MAE, MAPE of the ARIMA-GM combined model were smaller than that of a single model, and the combined model could improve the prediction accuracy. Using the combined model to forecast the incidence of hepatitis B during Jan 2013 to Dec 2013, the overall trend was relatively consistent with the condition of previous years. Conclusion The ARIMA-GM combined model can better fit the incidence rate of hepatitis B with a greater accuracy than the seasonal ARIMA model. The prediction results can provide the reference for the early warning system of HBV. DOI: 10.11855/j.issn.0577-7402.2014.01.12

  18. Probiotics protect mice from CoCrMo particles-induced osteolysis

    Directory of Open Access Journals (Sweden)

    Wang Z

    2017-07-01

    Full Text Available Zhenheng Wang,* Kaiwen Xue,* Maosheng Bai, Zhantao Deng, Jingjing Gan, Gang Zhou, Hongbo Qian,* Nirong Bao, Jianning Zhao Department of Orthopaedics, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, People’s Republic of China *These authors contributed equally to this work Abstract: Wear particle-induced inflammatory osteolysis is the primary cause of aseptic loosening, which is the most common reason for total hip arthroplasty (THA failure in the med- and long term. Recent studies have suggested an important role of gut microbiota (GM in modulating the host metabolism and immune system, leading to alterations in bone mass. Probiotic bacteria administered in adequate amounts can alter the composition of GM and confer health benefits to the host. Given the inflammatory osteolysis that occurs in wear debris-induced prosthesis loosening, we examined whether the probiotic Lactobacillus casei could reduce osteolysis in a mouse calvarial resorption model. In this study, L. casei markedly protected mice from CoCrMo particles (CoPs-induced osteolysis. Osteoclast gene markers and the number of osteoclasts were significantly decreased in L. casei-treated mice. Probiotic treatment decreased the M1-like macrophage phenotype indicated by downregulation of tumor necrosis factor α (TNF-α, interleukin (IL-6 and inducible nitric oxide synthase (iNOS and increased the M2-like macrophage phenotype indicated by upregulation of IL-4, IL-10 and arginase. Collectively, these results indicated that the L. casei treatment modulated the immune status and suppressed wear particle-induced osteolysis in vivo. Thus, probiotic treatment may represent a potential preventive and therapeutic approach to reduced wear debris-induced osteolysis. Keywords: wear particles, gut microbiota, nanotoxicity, macrophage polarization, inflammatory cytokines, aseptic loosening

  19. On employing H216O, H217O, H218O, and D216O lines as frequency standards in the 15-170 cm-1 window

    International Nuclear Information System (INIS)

    Furtenbacher, Tibor; Csaszar, Attila G.

    2008-01-01

    The protocol MARVEL, standing for measured active rotational-vibrational energy levels, is used to study high-accuracy measurements of rotational lines of four isotopologues of water, H 2 16 O, H 2 17 O, H 2 18 O, and D 2 16 O, obtained by spectroscopy in the far-infrared (FIR) region of 15-170 cm -1 by Matsushima et al. [Matsushima F, Odashima H, Iwasaki T, Tsunekawa S, Takagi K. Frequency measurement of pure rotational transitions of H 2 O from 0.5 to 5 THz. J Mol Struct 1995; 352/353, 371-8; Matsushima F, Nagase H, Nakauchi T, Odashima H, Takagi K. Frequency measurement of pure rotational transitions of H 2 17 O and H 2 18 O from 0.5 to 5 THz. J Mol Spectrosc 1999;193: 217-23; Matsushima F, Matsunaga M, Qian GY, Ohtaki Y, Wang RL, Takagi K. Frequency measurement of pure rotational transitions of D 2 O from 0.5 to 5 THz. J Mol Spectrosc 2001;206: 41-6; Matsushima F, Tomatsu N, Nagai T, Moriwaki Y, Takagi K. Frequency measurement of pure rotational transitions in the v 2 =1 state of H 2 O. J Mol Spectrosc 2006;235: 190-5]. MARVEL validates the high accuracy of most of the measured line positions. It results in a considerable number of energy levels with an average internal uncertainty of only 40 kHz (2σ). It also supports serious inaccuracy problems when Watson-type A-reduced Hamiltonians are used for predicting the highly accurate rotational measurements for water. Finally, MARVEL suggests a large number of para-water levels, for example 41 for H 2 16 O, which are candidates for becoming frequency standards in the FIR region of 15-170 cm -1 (the 0.5-5 THz window) when an accuracy of about 0.1 MHz is deemed to be sufficient

  20. Silver nanoparticles inhibit the function of hypoxia-inducible factor-1 and target genes: insight into the cytotoxicity and antiangiogenesis

    Directory of Open Access Journals (Sweden)

    Yang T

    2016-12-01

    Full Text Available Tieshan Yang,1 Qian Yao,1 Fei Cao,1 Qianqian Liu,1 Binlei Liu,2 Xiu-Hong Wang1 1Laboratory for Biomedical Photonics, Institute of Laser Engineering, Beijing University of Technology, 2Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People’s Republic of China Abstract: Hypoxia-inducible factor-1 (HIF-1 is a transcription factor that is activated upon exposure to hypoxic stress. It modulates a number of cellular responses including proliferation, apoptosis, angiogenesis, and metabolism by activating a panel of target genes in response to hypoxia. The HIF-1 level is often upregulated in the hypoxic microenvironment of solid tumors, which contributes to cancer treatment failure. Here we report that silver nanoparticles (AgNPs, which are widely used as an antimicrobial agent, are an effective inhibitor of HIF-1. AgNPs inhibited the activation of a HIF-dependent reporter construct after the cells were exposed to hypoxic conditions or treated with cobalt chloride, a hypoxia mimetic agent. The AgNPs also interfered with the accumulation of HIF-1α protein and the induction of the endogenous HIF target genes, VEGF-A and GLUT1. Since both HIF-1 and vascular endothelial growth factor-A play an important role in angiogenesis, AgNPs also inhibited angiogenesis in vitro. Our data reveal a new mechanism of how AgNPs act on cellular function, that is, they disrupt HIF signaling pathway. This finding provides a novel insight into how AgNPs can inhibit cancer cell growth and angiogenesis. Keywords: silver nanoparticles (AgNPs, hypoxia-inducible factor, transcriptional activity, vascular endothelial growth factor-A, angiogenesis

  1. Polyethylene glycol–polylactic acid nanoparticles modified with cysteine–arginine–glutamic acid–lysine–alanine fibrin-homing peptide for glioblastoma therapy by enhanced retention effect

    Directory of Open Access Journals (Sweden)

    Wu J

    2014-11-01

    Full Text Available Junzhu Wu,1,2,* Jingjing Zhao,1,3,* Bo Zhang,1 Yong Qian,1 Huile Gao,1 Yuan Yu,1 Yan Wei,1 Zhi Yang,1 Xinguo Jiang,1 Zhiqing Pang1 1Key Laboratory of Smart Drug Delivery, Ministry of Education, Department of Pharmaceutics, School of Pharmacy, Fudan University, Shanghai, 2School of Pharmacy, Dali University, Xiaguan, 3School of Pharmacy, China Pharmaceutical University, Nanjing, People’s Republic of China *These authors contributed equally to this work Abstract: For a nanoparticulate drug-delivery system, crucial challenges in brain-glioblastoma therapy are its poor penetration and retention in the glioblastoma parenchyma. As a prevailing component in the extracellular matrix of many solid tumors, fibrin plays a critical role in the maintenance of glioblastoma morphology and glioblastoma cell differentiation and proliferation. We developed a new drug-delivery system by conjugating polyethylene glycol–polylactic acid nanoparticles (NPs with cysteine–arginine–glutamic acid–lysine–alanine (CREKA; TNPs, a peptide with special affinity for fibrin, to mediate glioblastoma-homing and prolong NP retention at the tumor site. In vitro binding tests indicated that CREKA significantly enhanced specific binding of NPs with fibrin. In vivo fluorescence imaging of glioblastoma-bearing nude mice, ex vivo brain imaging, and glioblastoma distribution demonstrated that TNPs had higher accumulation and longer retention in the glioblastoma site over unmodified NPs. Furthermore, pharmacodynamic results showed that paclitaxel-loaded TNPs significantly prolonged the median survival time of intracranial U87 glioblastoma-bearing nude mice compared with controls, Taxol, and NPs. These findings suggested that TNPs were able to target the glioblastoma and enhance retention, which is a valuable strategy for tumor therapy. Keywords: CREKA peptide, nanoparticles, retention effect, paclitaxel, glioblastoma

  2. A novel spray-dried nanoparticles-in-microparticles system for formulating scopolamine hydrobromide into orally disintegrating tablets

    Directory of Open Access Journals (Sweden)

    Li FQ

    2011-04-01

    Full Text Available Feng-Qian Li1, Cheng Yan2, Juan Bi1, Wei-Lin Lv3, Rui-Rui Ji3, Xu Chen1, Jia-Can Su3, Jin-Hong Hu31Department of Pharmaceutics, Shanghai Eighth People’s Hospital, Shanghai, People’s Republic of China; 2Department of Pharmacy, Bethune International Peace Hospital, Shijiazhuang, People’s Republic of China; 3Changhai Hospital, Second Military Medical University, Shanghai, People’s Republic of ChinaAbstract: Scopolamine hydrobromide (SH-loaded microparticles were prepared from a colloidal fluid containing ionotropic-gelated chitosan nanoparticles using a spray-drying method. The spray-dried microparticles were then formulated into orally disintegrating tablets (ODTs using a wet granulation tablet formation process. A drug entrapment efficiency of about 90% (w/w and loading capacity of 20% (w/w were achieved for the microparticles, which ranged from 2 µm to 8 µm in diameter. Results of disintegration tests showed that the formulated ODTs could be completely dissolved within 45 seconds. Drug dissolution profiles suggested that SH is released more slowly from tablets made using the microencapsulation process compared with tablets containing SH that is free or in the form of nanoparticles. The time it took for 90% of the drug to be released increased significantly from 3 minutes for conventional ODTs to 90 minutes for ODTs with crosslinked microparticles. Compared with ODTs made with noncrosslinked microparticles, it was thus possible to achieve an even lower drug release rate using tablets with appropriate chitosan crosslinking. Results obtained indicate that the development of new ODTs designed with crosslinked microparticles might be a rational way to overcome the unwanted taste of conventional ODTs and the side effects related to SH’s intrinsic characteristics.Keywords: scopolamine hydrobromide, chitosan, nanoparticles-in-microparticles system, spray-drying, orally disintegrating tablets

  3. Biodegradable magnesium nanoparticle-enhanced laser hyperthermia therapy

    Directory of Open Access Journals (Sweden)

    Wang Q

    2012-08-01

    Full Text Available Qian Wang,1 Liping Xie,1 Zhizhu He,2 Derui Di,2 Jing Liu1,21Department of Biomedical Engineering, School of Medicine, Tsinghua University, 2Key Laboratory of Cryogenics, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, People's Republic of ChinaBackground: Recently, nanoparticles have been demonstrated to have tremendous merit in terms of improving the treatment specificity and thermal ablation effect on tumors. However, the potential toxicity and long-term side effects caused by the introduced nanoparticles and by expelling them out of the body following surgery remain a significant challenge. Here, we propose for the first time to directly adopt magnesium nanoparticles as the heating enhancer in laser thermal ablation to avoid these problems by making full use of the perfect biodegradable properties of this specific material.Methods: To better understand the new nano “green” hyperthermia modality, we evaluated the effects of magnesium nanoparticles on the temperature transients inside the human body subject to laser interstitial heating. Further, we experimentally investigated the heating enhancement effects of magnesium nanoparticles on a group of biological samples: oil, egg white, egg yolk, in vitro pig tissues, and the in vivo hind leg of rabbit when subjected to laser irradiation.Results: Both the theoretical simulations and experimental measurements demonstrated that the target tissues injected with magnesium nanoparticles reached much higher temperatures than tissues without magnesium nanoparticles. This revealed the enhancing behavior of the new nanohyperthermia method.Conclusion: Given the unique features of magnesium nanoparticles – their complete biological safety and ability to enhance heating – which most other advanced metal nanoparticles do not possess, the use of magnesium nanoparticles in hyperthermia therapy offers an important “green” nanomedicine modality for treating tumors

  4. Surrogate Model Application to the Identification of Optimal Groundwater Exploitation Scheme Based on Regression Kriging Method—A Case Study of Western Jilin Province

    Directory of Open Access Journals (Sweden)

    Yongkai An

    2015-07-01

    Full Text Available This paper introduces a surrogate model to identify an optimal exploitation scheme, while the western Jilin province was selected as the study area. A numerical simulation model of groundwater flow was established first, and four exploitation wells were set in the Tongyu county and Qian Gorlos county respectively so as to supply water to Daan county. Second, the Latin Hypercube Sampling (LHS method was used to collect data in the feasible region for input variables. A surrogate model of the numerical simulation model of groundwater flow was developed using the regression kriging method. An optimization model was established to search an optimal groundwater exploitation scheme using the minimum average drawdown of groundwater table and the minimum cost of groundwater exploitation as multi-objective functions. Finally, the surrogate model was invoked by the optimization model in the process of solving the optimization problem. Results show that the relative error and root mean square error of the groundwater table drawdown between the simulation model and the surrogate model for 10 validation samples are both lower than 5%, which is a high approximation accuracy. The contrast between the surrogate-based simulation optimization model and the conventional simulation optimization model for solving the same optimization problem, shows the former only needs 5.5 hours, and the latter needs 25 days. The above results indicate that the surrogate model developed in this study could not only considerably reduce the computational burden of the simulation optimization process, but also maintain high computational accuracy. This can thus provide an effective method for identifying an optimal groundwater exploitation scheme quickly and accurately.

  5. Efficacy of intraarterial chemoinfusion therapy for locally advanced breast cancer patients: a retrospective analysis of 28 cases

    Directory of Open Access Journals (Sweden)

    Zhang W

    2013-06-01

    Full Text Available Wei Zhang,1,* Rong Liu,1,* Yingying Wang,1 Sheng Qian,1 Jianhua Wang,1 Zhiping Yan,1 Hongwei Zhang21Department of Interventional Radiology, Zhongshan Hospital, Fudan University, Shanghai, People's Republic of China; 2Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai, People's Republic of China*These authors contributed equally to this workObjective: The objective of this study was to evaluate the outcome of image-guided delivery of intraarterially infused chemotherapeutic drugs for patients with locally advanced breast cancer.Methods: Twenty-eight patients with pathologically proven, locally advanced breast cancer received intraarterial chemoinfusion therapy (chemoinfusion with docetaxel 75 mg/m2 and epirubicin 50 mg/m2. Digital subtraction angiography was performed to determine tumor arterial blood supply and to guide chemotherapy infusion. Patients were evaluated for complete remission (CR and partial remission (PR.Results: Twenty-eight patients received a total of 64 intraarterial chemoinfusions, 20 patients (71.4% received two infusions, and eight patients (28.6% received three infusions. One patient (3.6% had CR and 23 (82.1% had PR. The total effectiveness rate (CR and PR was 85.7% (24/28. All stage 3 patients underwent Phase II surgical resection after chemoinfusion, and the surgical resection participation rate was 100% (26/26. The mean time from the first chemoinfusion to surgery was 2 ± 1.2 months. Two patients with stage 4 cancer died of distant metastasis and cachexia, and the remaining 26 patients were still alive.Conclusion: Intraarterial chemoinfusion is a safe and effective therapy, achieving down-staging in a relatively short period for locally advanced breast cancer.Keywords: advanced breast cancer, intraarterial infusion, chemotherapy, therapeutic effect

  6. Investigation of age-related decline of microfibril-associated glycoprotein-1 in human skin through immunohistochemistry study

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    Zheng Q

    2013-12-01

    Full Text Available Qian Zheng, Siming Chen, Ying Chen, John Lyga, Russell Wyborski, Uma SanthanamGlobal Research and Development, Avon Products Inc., Suffern, New York, USAAbstract: During aging, the reduction of elastic and collagen fibers in dermis can lead to skin atrophy, fragility, and aged appearance, such as increased facial wrinkling and sagging. Microfibril-associated glycoprotein-1 (MAGP-1 is an extracellular matrix protein critical for elastic fiber assembly. It integrates and stabilizes the microfibril and elastin matrix network that helps the skin to endure mechanical stretch and recoil. However, the observation of MAGP-1 during skin aging and its function in the dermis has not been established. To better understand age-related changes in the dermis, we investigated MAGP-1 during skin aging and photoaging, using a combination of in vitro and in vivo studies. Gene expression by microarray was performed using human skin biopsies from young and aged female donors. In addition, immunofluorescence analysis on the MAGP-1 protein was performed in dermal fibroblast cultures and in human skin biopsies. Specific antibodies against MAGP-1 and fibrillin-1 were used to examine protein expression and extracellular matrix structure in the dermis via biopsies from donors of multiple age groups. A reduction of the MAGP-1 gene and protein levels were observed in human skin with increasing age and photoexposure, indicating a loss of the functional MAGP-1 fiber network and a lack of structural support in the dermis. Loss of MAGP-1 around the hair follicle/pore areas was also observed, suggesting a possible correlation between MAGP-1 loss and enlarged pores in aged skin. Our findings demonstrate that a critical “pre-elasticity” component, MAGP-1, declines with aging and photoaging. Such changes may contribute to age-related loss of dermal integrity and perifollicular structural support, which may lead to skin fragility, sagging, and enlarged pores

  7. Characterization of the Bell-Shaped Vibratory Angular Rate Gyro

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    Junfang Fan

    2013-08-01

    Full Text Available The bell-shaped vibratory angular rate gyro (abbreviated as BVG is a novel shell vibratory gyroscope, which is inspired by the Chinese traditional bell. It sensitizes angular velocity through the standing wave precession effect. The bell-shaped resonator is a core component of the BVG and looks like the millimeter-grade Chinese traditional bell, such as QianLong Bell and Yongle Bell. It is made of Ni43CrTi, which is a constant modulus alloy. The exciting element, control element and detection element are uniformly distributed and attached to the resonator, respectively. This work presents the design, analysis and experimentation on the BVG. It is most important to analyze the vibratory character of the bell-shaped resonator. The strain equation, internal force and the resonator's equilibrium differential equation are derived in the orthogonal curvilinear coordinate system. When the input angular velocity is existent on the sensitive axis, an analysis of the vibratory character is performed using the theory of thin shells. On this basis, the mode shape function and the simplified second order normal vibration mode dynamical equation are obtained. The coriolis coupling relationship about the primary mode and secondary mode is established. The methods of the signal processing and control loop are presented. Analyzing the impact resistance property of the bell-shaped resonator, which is compared with other shell resonators using the Finite Element Method, demonstrates that BVG has the advantage of a better impact resistance property. A reasonable means of installation and a prototypal gyro are designed. The gyroscopic effect of the BVG is characterized through experiments. Experimental results show that the BVG has not only the advantages of low cost, low power, long work life, high sensitivity, and so on, but, also, of a simple structure and a better impact resistance property for low and medium angular velocity measurements.

  8. Effects of Laser Printer–Emitted Engineered Nanoparticles on Cytotoxicity, Chemokine Expression, Reactive Oxygen Species, DNA Methylation, and DNA Damage: A Comprehensive in Vitro Analysis in Human Small Airway Epithelial Cells, Macrophages, and Lymphoblasts

    Science.gov (United States)

    Pirela, Sandra V.; Miousse, Isabelle R.; Lu, Xiaoyan; Castranova, Vincent; Thomas, Treye; Qian, Yong; Bello, Dhimiter; Kobzik, Lester; Koturbash, Igor; Demokritou, Philip

    2015-01-01

    Background Engineered nanomaterials (ENMs) incorporated into toner formulations of printing equipment become airborne during consumer use. Although information on the complex physicochemical and toxicological properties of both toner powders and printer-emitted particles (PEPs) continues to grow, most toxicological studies have not used the actual PEPs but rather have primarily used raw toner powders, which are not representative of current exposures experienced at the consumer level during printing. Objectives We assessed the biological responses of a panel of human cell lines to PEPs. Methods Three physiologically relevant cell lines—small airway epithelial cells (SAECs), macrophages (THP-1 cells), and lymphoblasts (TK6 cells)—were exposed to PEPs at a wide range of doses (0.5–100 μg/mL) corresponding to human inhalation exposure durations at the consumer level of 8 hr or more. Following treatment, toxicological parameters reflecting distinct mechanisms were evaluated. Results PEPs caused significant membrane integrity damage, an increase in reactive oxygen species (ROS) production, and an increase in pro-inflammatory cytokine release in different cell lines at doses equivalent to exposure durations from 7.8 to 1,500 hr. Furthermore, there were differences in methylation patterns that, although not statistically significant, demonstrate the potential effects of PEPs on the overall epigenome following exposure. Conclusions The in vitro findings obtained in this study suggest that laser printer–emitted engineered nanoparticles may be deleterious to lung cells and provide preliminary evidence of epigenetic modifications that might translate to pulmonary disorders. Citation Pirela SV, Miousse IR, Lu X, Castranova V, Thomas T, Qian Y, Bello D, Kobzik L, Koturbash I, Demokritou P. 2016. Effects of laser printer–emitted engineered nanoparticles on cytotoxicity, chemokine expression, reactive oxygen species, DNA methylation, and DNA damage: a comprehensive in

  9. Time associated with intravenous zoledronic acid administration in patients with breast or prostate cancer and bone metastasis

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    Richhariya A

    2012-02-01

    Full Text Available Akshara Richhariya1, Yi Qian2, Yufan Zhao2, Karen Chung11Amgen Inc, Global Health Economics, Thousand Oaks, CA, USA; 2Amgen Inc, Global Biostatistical Sciences, Thousand Oaks, CA, USAPurpose: Intravenous (IV zoledronic acid (ZA is commonly used to delay skeletal complications secondary to bone metastases. However, the time associated with ZA administration may represent a significant burden to healthcare providers and patients. This study assessed the time associated with IV ZA infusion in patients with bone metastases secondary to breast or prostate cancer (BC or PC in the clinic setting.Methods: Eligible BC or PC patients with bone metastases scheduled to receive IV ZA were observed at seven US-based oncology clinics. Trained observers recorded the time for preinfusion tasks, ZA drug preparation, intravenous infusion, and follow-up activities.Results: Data are reported for 39 patients (BC: 24; PC: 15. Mean administration time was 69 (standard deviation [SD] 42 minutes for all patients combined, 72 (SD 47 minutes for BC, and 65 (SD 33 minutes for PC. Activity times were comparable between tumor types. Mean time for preinfusion tasks (eg, assessment of vital signs, blood draw and ZA preparation were 12 (SD 20 minutes and 2 (SD 1 minutes, respectively. Mean time required for intravenous infusion (ZA infusion and hydration, when provided and follow-up activities were 54 (SD 31 minutes and 2 (SD 1 minutes, respectively.Conclusion: Infusion time was the greatest time commitment associated with IV ZA administration, representing 78% of the total time on average. Time for preinfusion activities varied substantially. Overall, the mean time for ZA administration represents a notable time burden for healthcare providers and patients.Keywords: time and motion, bisphosphonates, zoledronic acid, intravenous administration

  10. Measurement of treatment adherence with antipsychotic agents in patients with schizophrenia

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    Xinhua S Ren

    2009-09-01

    Full Text Available Xinhua S Ren1,2,3, Lawrence Herz4,5, Shirley Qian1,2,3, Eric Smith3,4, Lewis E Kazis1,2,31The Center for the Assessment of Pharmaceutical Practices (CAPP, Boston University School of Public Health, Boston, MA, USA; 2Department of Health Policy and Management, Boston University School of Public Health, Boston, MA, USA; 3Center for Health Quality, Outcomes, and Economic Research, Bedford Veterans Affairs Medical Center, Bedford, MA, USA; 4Division of Psychiatry, Boston University School of Medicine, Boston, MA, USA; 5Mental Health Service Line, Bedford VA Medical Center, Bedford, MA, USAAbstract: The importance of medication adherence in sustaining control of schizophrenic symptoms has generated a great deal of interest in comparing levels of treatment adherence with different antipsychotic agents. However, the bulk of the research has yielded results that are often inconsistent. In this prospective, observational study, we assessed the measurement properties of 3 commonly used, pharmacy-based measures of treatment adherence with antipsychotic agents in schizophrenia using data from the Veterans Health Administration during 2000 to 2005. Patients were selected if they were on antipsychotics and diagnosed with schizophrenia (N = 18,425. A gap of ≥30 days (with no filled index medication was used to define discontinuation of treatment as well as medication “episodes,” or the number of times a patient returned to the same index agent after discontinuation of treatment within a 1-year period. The study found that the 3 existing measures differed in their approaches in measuring treatment adherence, suggesting that studies using these different measures would generate different levels of treatment adherence across antipsychotic agents. Considering the measurement problems associated with each existing approach, we offered a new, medication episode-specific approach, which would provide a fairer comparison of the levels of treatment adherence

  11. Antitumor effect of manumycin on colorectal cancer cells by increasing the reactive oxygen species production and blocking PI3K-AKT pathway

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    Zhang JY

    2016-05-01

    Full Text Available Jingyu Zhang,1 Hua Jiang,2 Li Xie,1 Jing Hu,1 Li Li,1 Mi Yang,1 Lei Cheng,1 Baorui Liu,1 Xiaoping Qian1 1Department of the Comprehensive Cancer Center, Affiliated Nanjing Drum Tower Hospital, Nanjing Medical University, 2Department of Oncology, Affiliated Changzhou No 2 People’s Hospital, Nanjing Medical University, Nanjing, People’s Republic of China Abstract: Manumycin is a natural, well-tolerated microbial metabolite and is regarded as a farnesyltransferase inhibitor. Some data suggest that manumycin inhibits proliferation of diverse cancer cells through various pathways. However, the antitumor effect of manumycin on colorectal cancer (CRC remains unknown. In the present study, we investigated the antitumor effect of manumycin on CRC in vitro and in vivo. The results of cell viability assay revealed that the proliferation of the CRC cells was significantly inhibited by manumycin. Moreover, cell apoptosis induced by manumycin was also found in a time- and dose-dependent manner. Interestingly, treatment of the CRC cells with manumycin resulted in increased generation of reactive oxygen species. Subsequently, manumycin also decreased the phosphorylation of phosphatidylinositol 3-kinase (PI3K and AKT, as well as the expression of caspase-9 and poly(ADP-ribose polymerase (PARP in a time-dependent manner. In addition, we found that N-acetyl-L-cysteine (NAC attenuated the effect of manumycin on the PI3K-AKT pathway, and wortmannin reduced the effect of manumycin on caspase-9 and PARP expression. More importantly, the anticancer effect of manumycin was also observed in established tumor xenografts. Taken together, these findings supported the potential application of manumycin against colorectal carcinoma. Keywords: manumycin, colorectal cancer, PI3K-AKT pathway, ROS

  12. Supplementary catalogue of the Anthomyiidae (Diptera of China

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    Mengmeng Wang

    2014-11-01

    Full Text Available The present catalogue of Anthomyiidae attempts to list all species (173 described or recorded from mainland China (165 and Taiwan (8 that for various reasons are not treated in “Flies of China” from 1998. The catalogue further lists Chinese species that are presently standing in new generic combinations compared to those of “Flies of China”, species that have changed name because of synonymy or misidentification, and species upgraded from subspecies to species. Regional distribution by province is specified for all species. Literature sources to descriptions or records of anthomyiid species from China are only given for those 173 species not covered by “Flies of China”. Four new combinations are proposed: Enneastigma fulva (Malloch, 1934, Enneastigma henanensis (Ge & Fan, 1982, Enneastigma lengshanensis (Xue, 2001 and Hylemya qinghaiensis (Fan, Chen & Ma, 1989. Eremomyia turbida Huckett, 1951 is revived from synonymy with Chortophila triticiperda Stein, 1900 (current name Eutrichota turbida. One subspecies is upgraded to species: Adia asiatica Fan, 1988. The following eight new synonymies are proposed: Delia pectinator fuscilateralis Fan in Fan & Zheng, 1992 with Delia pectinator Suwa, 1984; Eremomyia pilimana pilimarginata Fan & Qian in Fan, Chen, Ma & Ge, 1982 with Eremomyia turbida Huckett, 1951 (current name Eutrichota turbida; Lopesohylemya Fan, Chen & Ma, 1989 with Hylemya Robineau-Desvoidy, 1830; Deliomyia Fan in Fan et al., 1988 with Subhylemyia Ringdahl, 1933; Hydrophoria disticrassa Xue & Bai, 2009 with Hydrophoria pullata Wu, Liu & Wei, 1995 (current name Zaphne pullata; Heteroterma Wei, 2006 with Scathophaga Meigen, 1803; Heteroterma fanjingensis Wei, 2006 with Scathophaga curtipilata Feng, 2002; Scatomyza fansipanicola Ozerov in Ozerov & Krivosheina, 2011 with Scathophaga curtipilata Feng, 2002. The genus Heteroterma Wei, 2006 and species Heteroterma fanjingensis Wei, 2006 are reassigned from Anthomyiidae to

  13. GX1-conjugated poly(lactic acid nanoparticles encapsulating Endostar for improved in vivo anticolorectal cancer treatment

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    Du Y

    2015-05-01

    Full Text Available Yang Du,1,* Qian Zhang,1,* Lijia Jing,2 Xiaolong Liang,2 Chongwei Chi,1 Yaqian Li,1 Xin Yang,1 Zhifei Dai,2 Jie Tian1 1Key Laboratory of Molecular Imaging, The State Key Laboratory of Management and Control for Complex Systems, Institute of Automation, Chinese Academy of Sciences, Beijing, People’s Republic of China; 2Department of Biomedical Engineering, College of Engineering, Peking University, Beijing People’s Republic of China *These authors contributed equally to this work Abstract: Tumor angiogenesis plays a key role in tumor growth and metastasis; thus, targeting tumor-associated angiogenesis is an important goal in cancer therapy. However, the efficient delivery of drugs to tumors remains a key issue in antiangiogenesis therapy. GX1, a peptide identified by phage-display technology, is a novel tumor vasculature endothelium-specific ligand and possesses great potential as a targeted vector and antiangiogenic agent in the diagnosis and treatment of human cancers. Endostar, a novel recombinant human endostatin, has been shown to inhibit tumor angiogenesis. In this study, we developed a theranostic agent composed of GX1-conjugated poly(lactic acid nanoparticles encapsulating Endostar (GPENs and labeled with the near-infrared dye IRDye 800CW to improve colorectal tumor targeting and treatment efficacy in vivo. The in vivo fluorescence molecular imaging data showed that GPENs (IRDye 800CW more specifically targeted tumors than free IRDye 800CW in colorectal tumor-bearing mice. Moreover, the antitumor efficacy was evaluated by bioluminescence imaging and immunohistology, revealing that GPENs possessed improved antitumor efficacy on subcutaneous colorectal xenografts compared to other treatment groups. Thus, our study showed that GPENs, a novel GX1 peptide guided form of nanoscale Endostar, can be used as a theranostic agent to facilitate more efficient targeted therapy and enable real-time monitoring of therapeutic efficacy in vivo

  14. Gene therapy for C-26 colon cancer using heparin-polyethyleneimine nanoparticle-mediated survivin T34A

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    Zhang L

    2011-10-01

    Full Text Available Ling Zhang1,*, Xiang Gao1,2,*, Ke Men1, BiLan Wang1, Shuang Zhang1, Jinfeng Qiu1, Meijuan Huang1, MaLing Gou1, Ning Huang2, ZhiYong Qian1, Xia Zhao1, YuQuan Wei11State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, 2Department of Pathophysiology, College of Preclinical and Forensic Medical Sciences, Sichuan University, Chengdu, People’s Republic of China*These authors contributed equally to this workBackground: Gene therapy provides a novel method for the prevention and treatment of cancer, but the clinical application of gene therapy is restricted, mainly because of the absence of an efficient and safe gene delivery system. Recently, we developed a novel nonviral gene carrier, ie, heparin-polyethyleneimine (HPEI nanoparticles for this purpose.Methods and results: HPEI nanoparticles were used to deliver plasmid-expressing mouse survivin-T34A (ms-T34A to treat C-26 carcinoma in vitro and in vivo. According to the in vitro studies, HPEI nanoparticles could efficiently transfect the pGFP report gene into C-26 cells, with a transfection efficiency of 30.5% ± 2%. Moreover, HPEI nanoparticle-mediated ms-T34A could efficiently inhibit the proliferation of C-26 cells by induction of apoptosis in vitro. Based on the in vivo studies, HPEI nanoparticles could transfect the Lac-Z report gene into C-26 cells in vivo. Intratumoral injection of HPEI nanoparticle-mediated ms-T34A significantly inhibited growth of subcutaneous C-26 carcinoma in vivo by induction of apoptosis and inhibition of angiogenesis.Conclusion: This research suggests that HPEI nanoparticle-mediated ms-T34A may have a promising role in C-26 colon carcinoma therapy.Keywords: gene therapy, mouse survivin-T34A, colon cancer, polyethyleneimine, nanoparticles, cancer therapy

  15. Improved performance of diatomite-based dental nanocomposite ceramics using layer-by-layer assembly

    Directory of Open Access Journals (Sweden)

    Lu X

    2012-04-01

    Full Text Available Xiaoli Lu1,2, Yang Xia1, Mei Liu1, Yunzhu Qian3, Xuefeng Zhou4, Ning Gu4, Feimin Zhang1,41Institute of Stomatology, Nanjing Medical University, Nanjing, 2Nantong Stomatological Hospital, Nantong, 3Center of Stomatology, The Second Affiliated Hospital of Suzhou University, Suzhou, 4Suzhou Institute, Southeast University, Suzhou, People's Republic of ChinaAbstract: To fabricate high-strength diatomite-based ceramics for dental applications, the layer-by-layer technique was used to coat diatomite particles with cationic [poly(allylamine hydrochloride] and anionic [poly(sodium 4-styrenesulfonate] polymers to improve the dispersion and adsorption of positively charged nano-ZrO2 (zirconia as a reinforcing agent. The modified diatomite particles had reduced particle size, narrower size distribution, and were well dispersed, with good adsorption of nano-ZrO2. To determine the optimum addition levels for nano-ZrO2, ceramics containing 0, 20, 25, 30, and 35 wt% nano-ZrO2 were sintered and characterized by the three-point bending test and microhardness test. In addition to scanning electron microscopy, propagation phase-contrast synchrotron X-ray microtomography was used to examine the internal structure of the ceramics. The addition of 30 wt% nano-ZrO2 resulted in the highest flexural strength and fracture toughness with reduced porosity. Shear bond strength between the core and veneer of our diatomite ceramics and the most widely used dental ceramics were compared; the shear bond strength value for the diatomite-based ceramics was found to be significantly higher than for other groups (P < 0.05. Our results show that diatomite-based nanocomposite ceramics are good potential candidates for ceramic-based dental materials.Keywords: layer-by-layer, diatomite, nanoceramics, zirconia (ZrO2, dental materials

  16. Changing patient safety culture in China: a case study of an experimental Chinese hospital from a comparative perspective

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    Xu XP

    2018-05-01

    Full Text Available Xiao Ping Xu,* Dong Ning Deng,* Yong Hong Gu, Chui Shan Ng, Xiao Cai, Jun Xu, Xin Shi Zhang, Dong Ge Ke, Qian Hui Yu, Chi Kuen Chan Clinical Service Department, The University of Hong Kong - Shenzhen Hospital, Shenzhen, Guangdong, People’s Republic of China *These authors contributed equally to this work Background: The World Health Organization highlights that patient safety interventions are not lacking but that the local context affects their successful implementation. Increasing attention is being paid to patient safety in Mainland China, yet few studies focus on patient safety in organizations with mixed cultures. This paper evaluates the current patient safety culture in an experimental Chinese hospital with a Hong Kong hospital management culture, and it aims to explore the application of Hong Kong’s patient safety strategies in the context of Mainland China. Methods: A quantitative survey of 307 hospital staff members was conducted using the Hospital Survey on Patient Safety Culture questionnaire. The findings were compared with a similar study on general Chinese hospitals and were appraised with reference to the Manchester Patient Safety Framework. Results: Lower scores were observed among participants with the following characteristics: males, doctors, those with more work experience, those with higher education, and those from the general practice and otolaryngology departments. However, the case study hospital achieved better scores in management expectations, actions and support for patient safety, incident reporting and communication, and teamwork within units. Its weaknesses were related to non-punitive responses to errors, teamwork across units, and staffing. Conclusions: The case study hospital contributes to a changing patient safety culture in Mainland China, yet its patient safety culture remains mostly bureaucratic. Further efforts could be made to deepen the staff’s patient safety culture mind-set, to realize a

  17. Recombinant high-density lipoprotein nanoparticles containing gadolinium-labeled cholesterol for morphologic and functional magnetic resonance imaging of the liver

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    Rui M

    2012-07-01

    Full Text Available Mengjie Rui,1 Wei Guo,2 Qian Ding,2 Xiaohui Wei,2 Jianrong Xu,3 Yuhong Xu21School of Life Science and Biotechnology, 2School of Pharmacy, Shanghai Jiao Tong University, Shanghai, People's Republic of China; 3Department of Radiology, Renji Hospital Affiliation with Medical School of Shanghai Jiao Tong University, Shanghai, People's Republic of ChinaBackground: Natural high-density lipoproteins (HDL possess important physiological functions to the transport of cholesterol from the peripheral tissues to the liver for metabolic degradation and excretion in the bile.Methods and results: In this work, we took advantage of this pathway and prepared two different gadolinium (Gd-DTPA-labeled cholesterol-containing recombinant HDL nanoparticles (Gd-chol-HDL and Gd-(chol2-HDL as liver-specific magnetic resonance imaging (MRI contrast agents. The reconstituted HDL nanoparticles had structural similarity to native HDL, and could be taken up by HepG2 cells via interaction with HDL receptors in vitro. In vivo MRI studies in rats after intravenous injections of 10 µmol gadolinium per kg of recombinant HDL nanoparticles indicated that both nanoparticles could provide signal enhancement in the liver and related organs. However, different T1-weighted image details suggested that they participated in different cholesterol metabolism and excretion pathways in the liver.Conclusion: Such information could be highly useful to differentiate functional changes as well as anatomic differences in the liver. These cholesterol-derived contrast agents and their recombinant HDL preparations may warrant further development as a new class of contrast agents for MRI of the liver and related organs.Keywords: magnetic resonance imaging, apolipoprotein, high-density lipoprotein, contrast agent, gadolinium, liver

  18. A new pulmonary rehabilitation maintenance strategy through home-visiting and phone contact in COPD

    Directory of Open Access Journals (Sweden)

    Li Y

    2018-01-01

    Full Text Available Yi Li,1,2 Jing Feng,3,4 Yuechuan Li,2 Wei Jia,2 Hongyu Qian2 1Graduate School, Tianjin Medical University, 2Department of Respiratory and Critical Care Medicine, Tianjin Chest Hospital, 3Respiratory Department, Tianjin Medical University General Hospital, Tianjin, People’s Republic of China; 4Neuropharmacology Section, Laboratory of Toxicology and Pharmacology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC, USA Background: The benefit of pulmonary rehabilitation (PR for patients with COPD diminishes over time. We investigated a new strategy involving home-visit and phone contact and compared this to usual care in maintenance of PR benefits.Methods: A total of 172 stable COPD patients receiving 8-week PR program were recruited for this prospective study. Patients were allocated into usual care group (UC and PR maintenance group (PRMG randomly. Patients in PRMG participated in maintenance strategy at home under supervision through home-visit and phone contact. The 6-minute walking test (6MWT, COPD assessment test (CAT, and modified Medical Research Council scale (mMRC scores were evaluated every 3 months.Results: Of the total, 151 patients completed 8-week PR program with satisfactory PR results (p<0.001, and 104 patients finished the follow-up. The clinical improvements in 6MWT, CAT, and mMRC scores were maintained (p<0.001 in PRMG. In comparison, the benefit of PR diminished gradually in UC. The differences in 6MWT, CAT, and mMRC scores between groups were observed 6, 9, and 6 months after PR, respectively (p<0.05. Total frequency of exacerbations in PRMG was lower than UC (p=0.021.Conclusion: Maintenance strategy involving home-visit and phone contact is superior to usual care to preserve PR benefits, and reduces the acute COPD exacerbation rate. Keywords: COPD, pulmonary rehabilitation, 6-minute walking test, COPD assessment test, maintenance 

  19. Ambient Air Pollution and Adverse Pregnancy Outcomes in Wuhan, China.

    Science.gov (United States)

    Qian, Zhengmin; Zhang, Bin; Liang, Shengwen; Wang, Jing; Yang, Shaoping; Hu, Ke; Trevathan, Edwin; Yang, Rong; Li, Qijie; Flick, Louise H; Hu, Ronghua; Huang, Zhen; Zhang, Yimin; Hu, Shixiang; Wang, Jing; Shen, Longjiao; Lu, Yuan; Peng, Hui; Yu, Yuzhen; Yang, Li; Chen, Wei; Liu, Wenjin; Zhang, Wei

    2016-09-01

    Several recent studies have suggested that maternal exposures to air pollution and temperature extremes might contribute to low birth weight (LBW), preterm birth (PTB), and other outcomes that can adversely affect infant health. At the time the current study began, most other studies had been conducted in the United States or Europe. Dr. Zhengmin Qian proposed to extend work he had done on ambient particulate air pollution and daily mortality in Wuhan, China (Qian et al. 2010), as part of the HEIsponsored Public Health and Air Pollution in Asia program, to study adverse birth outcomes. Wuhan is the capital city of Hubei province, has a large population of about 6.4 million within the urban study area, experiences temperature extremes, and generally has higher air pollution levels than those observed in the United States and Europe, thus providing a good opportunity to explore questions about air pollution and health. Qian and colleagues planned a cohort and nested case–control design with four specific aims, examining whether increased exposures to air pollutants (PM2.5, PM10, SO2, NO2, O3, and CO) during vulnerable pregnancy periods were associated with increased rates of PTB, LBW (air pollution and daily weather data for August 2010 to June 2013 from nine monitoring stations representing background air pollution sites in seven Wuhan inner-city districts. Only two of these stations provided PM2.5 data. For the cohort study, the investigators assigned exposures to mothers according to the daily mean concentrations from the monitor nearest the residential community in which the mother lived at the time of the birth. For the case–control study, they assigned exposures based on the inverse distance weighted average of daily mean concentrations from the three nearest monitors, for all but PM2.5 for which the method was not specified. They also collected data on various factors that might confound or modify the impact of the pollutants on the adverse outcomes

  20. PREFACE: Exploring surfaces and buried interfaces of functional materials by advanced x-ray and neutron techniques Exploring surfaces and buried interfaces of functional materials by advanced x-ray and neutron techniques

    Science.gov (United States)

    Sakurai, Kenji

    2010-12-01

    measurement. Advances in such technologies are bringing with them new opportunities in surface and buried interface science. In the not too distant future, we will publish a special issue or a book detailing such progress. Exploring surfaces and buried interfaces of functional materials by advanced x-ray and neutron techniques contents Lateral uniformity in chemical composition along a buried reaction front in polymers using off-specular reflectivity Kristopher A Lavery, Vivek M Prabhu, Sushil Satija and Wen-li Wu Orientation dependence of Pd growth on Au electrode surfaces M Takahasi, K Tamura, J Mizuki, T Kondo and K Uosaki A grazing incidence small-angle x-ray scattering analysis on capped Ge nanodots in layer structures Hiroshi Okuda, Masayuki Kato, Keiji Kuno, Shojiro Ochiai, Noritaka Usami, Kazuo Nakajima and Osami Sakata High resolution grazing-incidence in-plane x-ray diffraction for measuring the strain of a Si thin layer Kazuhiko Omote X-ray analysis of mesoporous silica thin films templated by Brij58 surfactant S Fall, M Kulij and A Gibaud Review of the applications of x-ray refraction and the x-ray waveguide phenomenon to estimation of film structures Kouichi Hayashi Epitaxial growth of largely mismatched crystals on H-terminated Si(111) surfaces Hidehito Asaoka Novel TiO2/ZnO multilayer mirrors at 'water-window' wavelengths fabricated by atomic layer epitaxy H Kumagai, Y Tanaka, M Murata, Y Masuda and T Shinagawa Depth-selective structural analysis of thin films using total-external-reflection x-ray diffraction Tomoaki Kawamura and Hiroo Omi Structures of Yb nanoparticle thin films grown by deposition in He and N2 gas atmospheres: AFM and x-ray reflectivity studies Martin Jerab and Kenji Sakurai Ga and As composition profiles in InP/GaInAs/InP heterostructures—x-ray CTR scattering and cross-sectional STM measurements Yoshikazu Takeda, Masao Tabuchi and Arao Nakamura Polarized neutron reflectivity study of a thermally treated MnIr/CoFe exchange bias system Naoki

  1. Public reporting influences antibiotic and injection prescription in primary care: a segmented regression analysis.

    Science.gov (United States)

    Liu, Chenxi; Zhang, Xinping; Wan, Jie

    2015-08-01

    Inappropriate use and overuse of antibiotics and injections are serious threats to the global population, particularly in developing countries. In recent decades, public reporting of health care performance (PRHCP) has been an instrument to improve the quality of care. However, existing evidence shows a mixed effect of PRHCP. This study evaluated the effect of PRHCP on physicians' prescribing practices in a sample of primary care institutions in China. Segmented regression analysis was used to produce convincing evidence for health policy and reform. The PRHCP intervention was implemented in Qian City that started on 1 October 2013. Performance data on prescription statistics were disclosed to patients and health workers monthly in 10 primary care institutions. A total of 326 655 valid outpatient prescriptions were collected. Monthly effective prescriptions were calculated as analytical units in the research (1st to 31st every month). This study involved multiple assessments of outcomes 13 months before and 11 months after PRHCP intervention (a total of 24 data points). Segmented regression models showed downward trends from baseline on antibiotics (coefficient = -0.64, P = 0.004), combined use of antibiotics (coefficient = -0.41, P < 0.001) and injections (coefficient = -0.5957, P = 0.001) after PRHCP intervention. The average expenditure of patients slightly increased monthly before the intervention (coefficient = 0.8643, P < 0.001); PRHCP intervention also led to a temporary increase in average expenditure of patients (coefficient = 2.20, P = 0.307) but slowed down the ascending trend (coefficient = -0.45, P = 0.033). The prescription rate of antibiotics and injections after intervention (about 50%) remained high. PRHCP showed positive effects on physicians' prescribing behaviour, considering the downward trends on the use of antibiotics and injections and average expenditure through the intervention. However, the effect

  2. Downregulation of long noncoding RNA TUG1 inhibits proliferation and induces apoptosis through the TUG1/miR-142/ZEB2 axis in bladder cancer cells

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    Liu Q

    2017-05-01

    Full Text Available Qian Liu,* Hui Liu,* Hepeng Cheng, Yang Li, Xiaodong Li, Chaoyang Zhu Department of Urology Surgery, Huaihe Hospital of Henan University, Kaifeng, People’s Republic of China *These authors contributed equally to this work Background: Bladder cancer is a common serious disease around the world. Long noncoding RNAs (lncRNAs have been demonstrated to participate in the development and progression of various cancers, including bladder cancer. The aim of this study was to investigate the effects of lncRNA taurine upregulated gene 1 (TUG1 on proliferation and apoptosis in bladder cancer cell lines and the underlying mechanism.Methods: The levels of TUG1 were detected by quantitative real time polymerase chain reaction (qRT-PCR in bladder cancer tissues and cells. The mRNA and protein levels of zinc finger E-box binding homeobox 2 (ZEB2 were measured by qRT-PCR and Western blot analysis, respectively. The functional targets of TUG1 were predicted by online softwares and confirmed by luciferase reporter assay. The effects of TUG1 on cell proliferation and apoptosis were examined by MTT and apoptosis assay, respectively. The expression levels of β-catenin, cyclinD1, and c-Myc in T24 cells were determined by Western blot analysis.Results: The levels of TUG1 and ZEB2 were significantly increased in bladder cancer tissues and cells. Knockdown of either TUG1 or ZEB2 inhibited proliferation and induced apoptosis in bladder cancer cells. Interestingly, ZEB2 overexpression reversed the effects of TUG1 knockdown on cell proliferation and apoptosis. Moreover, ZEB2 was verified as a direct target of miR-142 and miR-142 could specially bind to TUG1. In addition, downregulation of TUG1 inhibited the Wnt/β-catenin pathway by regulating ZEB2 expression in bladder cancer cells.Conclusion: Downregulation of TUG1 expression inhibited proliferation and induced apoptosis in bladder cancer cells by targeting ZEB2 mediated by miR-142 through the inactivation of Wnt

  3. Greener synthesis of electrospun collagen/hydroxyapatite composite fibers with an excellent microstructure for bone tissue engineering

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    Zhou YY

    2015-04-01

    Full Text Available Yuanyuan Zhou,1,2 Hongchang Yao,1 Jianshe Wang,1 Dalu Wang,1 Qian Liu,1 Zhongjun Li11College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou, People’s Republic of China; 2Institute of Enviromental and Municipal Engineering, North China University of Water Resources and Electric Power, Zhengzhou, People’s Republic of ChinaAbstract: In bone tissue engineering, collagen/hydroxyapatite (HAP fibrous composite obtained via electrospinning method has been demonstrated to support the cells’ adhesion and bone regeneration. However, electrospinning of natural collagen often requires the use of cytotoxic organic solvents, and the HAP crystals were usually aggregated and randomly distributed within a fibrous matrix of collagen, limiting their clinical potential. Here, an effective and greener method for the preparation of collagen/HAP composite fibers was developed for the first time, and this green product not only had 40 times higher mechanical properties than that previously reported, but also had an excellent microstructure similar to that of natural bone. By dissolving type I collagen in environmentally friendly phosphate buffered saline/ethanol solution instead of the frequently-used cytotoxic organic solvents, followed with the key step of desalination, co-electrospinning the collagen solution with the HAP sol, generates a collagen/HAP composite with a uniform and continuous fibrous morphology. Interestingly, the nano-HAP needles were found to preferentially orient along the longitudinal direction of the collagen fibers, which mimicked the nanostructure of natural bones. Based on the characterization of the related products, the formation mechanism for this novel phenomenon was proposed. After cross-linking with 1-ethyl-3-(3-dimethyl-aminopropyl-1-carbodiimide hydrochloride/N-hydroxysuccinimide, the obtained composite exhibited a significant enhancement in mechanical properties. In addition, the biocompatibility of the

  4. HIV Trends in the United States: Diagnoses and Estimated Incidence.

    Science.gov (United States)

    Hall, H Irene; Song, Ruiguang; Tang, Tian; An, Qian; Prejean, Joseph; Dietz, Patricia; Hernandez, Angela L; Green, Timothy; Harris, Norma; McCray, Eugene; Mermin, Jonathan

    2017-02-03

    race or ethnicity groups. Further progress depends on effectively reducing HIV incidence among MSM, among whom the majority of new infections occur. ©H Irene Hall, Ruiguang Song, Tian Tang, Qian An, Joseph Prejean, Patricia Dietz, Angela L Hernandez, Timothy Green, Norma Harris, Eugene McCray, Jonathan Mermin. Originally published in JMIR Public Health and Surveillance (http://publichealth.jmir.org), 03.02.2017.

  5. D-α-tocopherol polyethylene glycol succinate-based derivative nanoparticles as a novel carrier for paclitaxel delivery

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    Wu YP

    2015-08-01

    Full Text Available Yupei Wu,1,* Qian Chu,2,* Songwei Tan,1 Xiangting Zhuang,1 Yuling Bao,1 Tingting Wu,1 Zhiping Zhang1,3,41Tongji School of Pharmacy, 2Department of Oncology, Tongji Hospital, Tongji Medical School, 3Hubei Engineering Research Center for NDDS, 4National Engineering Research Center for Nanomedicine, Huazhong University of Science and Technology, Wuhan, People’s Republic of China*These authors contributed equally to this workAbstract: Paclitaxel (PTX is one of the most effective antineoplastic drugs. Its current clinical administration Taxol® is formulated in Cremophor EL, which causes serious side effects. Nanoparticles (NP with lower systemic toxicity and enhanced therapeutic efficiency may be an alternative formulation of the Cremophor EL-based vehicle for PTX delivery. In this study, novel amphipathic 4-arm-PEG-TPGS derivatives, the conjugation of D-α-tocopherol polyethylene glycol succinate (TPGS and 4-arm-polyethylene glycol (4-arm-PEG with different molecular weights, have been successfully synthesized and used as carriers for the delivery of PTX. These 4-arm-PEG-TPGS derivatives were able to self-assemble to form uniform NP with PTX encapsulation. Among them, 4-arm-PEG5K-TPGS NP exhibited the smallest particle size, highest drug-loading efficiency, negligible hemolysis rate, and high physiologic stability. Therefore, it was chosen for further in vitro and in vivo investigations. Facilitated by the effective uptake of the NP, the PTX-loaded 4-arm-PEG5K-TPGS NP showed greater cytotoxicity compared with free PTX against human ovarian cancer (A2780, non-small cell lung cancer (A549, and breast adenocarcinoma cancer (MCF-7 cells, as well as a higher apoptotic rate and a more significant cell cycle arrest effect at the G2/M phase in A2780 cells. More importantly, PTX-loaded 4-arm-PEG5K-TPGS NP resulted in a significantly improved tumor growth inhibitory effect in comparison to Taxol® in S180 sarcoma-bearing mice models. This study suggested

  6. Cognitive effects of electro-acupuncture and pregabalin in a trigeminal neuralgia rat model induced by cobra venom

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    Chen RW

    2017-08-01

    Full Text Available Ruo-Wen Chen,1,2 Hui Liu,2 Jian-Xiong An,1,2 Xiao-Yan Qian,2 Yi-De Jiang,2 Doris K Cope,3 John P Williams,3 Rui Zhang,1 Li-Na Sun1 1Department of Anesthesiology, Weifang Medical University, Weifang City, Shandong, 2Department of Anesthesiology, Pain Medicine and Critical Care Medicine, Aviation General Hospital of China Medical University and Beijing Institute of Translational Medicine, Chinese Academy of Sciences, Beijing, China; 3Department of Anesthesiology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA Objective: The objective of this study was to investigate the effects of electro-acupuncture (EA and pregabalin on cognition impairment induced by chronic trigeminal neuralgia (TN in rats. Design: Controlled animal study. Setting: Department of Anesthesiology, Pain Medicine and Critical Care Medicine, Aviation General Hospital of China Medical University. Subjects: Forty adult male Sprague Dawley rats. Methods: Rats were randomly divided into four groups. The TN model was induced by administration of cobra venom to the left infraorbital nerve. On postoperative day 14, either EA or pregabalin was administered, free behavioral activities were observed. Spatial learning and memory abilities were determined in the Morris water maze. The ultrastructural alterations of the Gasserian ganglion, medulla oblongata and hippocampus were examined by electron microscopy. The changes on long-term potentiation were investigated. Results: After treatment, the exploratory behavior increased and the grooming behavior decreased (P<0.05 for the EA group and pregabalin group compared with the cobra venom group; moreover, demyelination of neurons in Gasserian ganglion and medulla oblongata was reversed. The number of platform site crossings, the average percentages of time in the target quadrant and the field excitatory postsynaptic potential slopes increased (P<0.05 in the EA group compared to the cobra venom group. However, the pregabalin group

  7. Improving the osteogenesis of human bone marrow mesenchymal stem cell sheets by microRNA-21-loaded chitosan/hyaluronic acid nanoparticles via reverse transfection

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    Wang Z

    2016-05-01

    Full Text Available Zhongshan Wang,1 Guangsheng Wu,2,3 Mengying Wei,4 Qian Liu,1 Jian Zhou,1 Tian Qin,1 Xiaoke Feng,1 Huan Liu,1 Zhihong Feng,1 Yimin Zhao1 1State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Oral Diseases, Department of Prosthodontics, 2State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Engineering Research Center for Dental Materials and Advanced Manufacture, Department of Periodontology, School of Stomatology, The Fourth Military Medical University, Xi’an, 3Qingdao First Sanatorium, Jinan Military Region, Qingdao, Shandong Province, 4Department of Biochemistry and Molecular Biology, The Fourth Military Medical University, Xi’an, People’s Republic of China Abstract: Cell sheet engineering has emerged as a novel approach to effectively deliver seeding cells for tissue regeneration, and developing human bone marrow mesenchymal stem cell (hBMMSC sheets with high osteogenic ability is a constant requirement from clinics for faster and higher-quality bone formation. In this work, we fabricated biocompatible and safe chitosan (CS/hyaluronic acid (HA nanoparticles (NPs to deliver microRNA-21 (miR-21, which has been proved to accelerate osteogenesis in hBMMSCs; then, the CS/HA/miR-21 NPs were cross-linked onto the surfaces of culture plates with 0.2% gel solution to fabricate miR-21-functionalized culture plates for reverse transfection. hBMMSC sheets were induced continuously for 14 days using a vitamin C-rich method on the miR-21-functionalized culture plates. For the characterization of CS/HA/miR-21 NPs, the particle size, zeta potential, surface morphology, and gel retardation were sequentially investigated. Then, the biological effects of hBMMSC sheets on the miR-21-functionalized culture plates were evaluated. The assay results demonstrated that the hBMMSC sheets could be successfully induced via the novel

  8. The efficacy and safety of alectinib in the treatment of ALK+ NSCLC: a systematic review and meta-analysis

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    Fan JS

    2018-03-01

    Full Text Available Junsheng Fan,1,2,* Zengfei Xia,1,* Xiaoli Zhang,1,* Yuqing Chen,1 Ruolan Qian,1 Sihan Liu,1 Danming You,1 Jian Zhang,1 Peng Luo1 1Department of Oncology, Zhujiang Hospital of Southern Medical University, Guangzhou, China; 2Department of Respiratory Medicine, Shanghai Tenth People’s Hospital, Tongji University, Shanghai, China *These authors contributed equally to this work Background: Alectinib is a second-generation anaplastic lymphoma kinase (ALK inhibitor approved by the US Food and Drug Administration to treat crizotinib-refractory non-small cell lung cancer. We performed this meta-analysis to synthesize the results of different clinical trials to evaluate the efficacy and safety of alectinib. Methods: A search of 3 databases, including PubMed, Web of Science, and the Cochrane Library, was performed from the inception of each database through September 5, 2017. We have pooled the overall response rate (ORR, disease control rate, progression-free survival, and intracranial ORR to evaluate the efficacy of alectinib. Discontinuation rate, rate of dose reduction or interruption due to adverse events as well as the incidence of several adverse events were aggregated to evaluate its safety. Results: A total of 8 studies with 626 patients have been included in our study. The pooled efficacy parameters are as follows: ORR 70% (95% CI: 57% to 82%, disease control rate 88% (95% CI: 82% to 94%, progression-free survival 9.36 months (95% CI: 7.38% to 11.34%, and intracranial ORR 52% (95% CI: 45% to 59%. ALK inhibitor-naïve patients tend to have better responses than crizotinib-pretreated patients. The aggregate discontinuation rate is 7% (95% CI: 4% to 10%, and the pooled rate of dose reduction or interruption is 33% (95% CI: 24% to 42%. The incidences of most adverse events were relatively low, while the incidences of 2 frequently reported adverse events, myalgia (18% and anemia (25%, were even higher than with the first-generation ALK inhibitor

  9. Intensity-modulated radiotherapy plus nimotuzumab with or without concurrent chemotherapy for patients with locally advanced nasopharyngeal carcinoma

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    Huang JF

    2017-12-01

    Full Text Available Jianfeng Huang,1,* Qinzhou Zou,1,* Danqi Qian,1 Leyuan Zhou,1 Bo Yang,1 Jianjun Chu,1 Qingfeng Pang,2 Kewei Wang,2 Fuzheng Zhang1 1Department of Radiation Oncology, Affiliated Hospital of Jiangnan University, 2Department of Epidemiology, Wuxi Medical School of Jiangnan University, Wuxi, Jiangsu, People’s Republic of China *These authors contributed equally to this work Objective: This study aimed to evaluate the safety and efficacy of intensity-modulated radiotherapy (IMRT plus nimotuzumab with or without concurrent chemotherapy (CCT for patients with locally advanced nasopharyngeal carcinoma (LA-NPC. Patients and methods: A total of 50 newly diagnosed patients with LA-NPC treated at the Affiliated Hospital of Jiangnan University between November 2011 and January 2017 were retrospectively analyzed. All patients received the combined treatment modality of nimotuzumab plus IMRT. Nimotuzumab was administered concurrently with IMRT at a weekly dose of 200 mg. Neoadjuvant, concurrent or adjuvant chemotherapy with the doublet regimen of taxanes (docetaxel or paclitaxel plus platinum (cisplatin or nedaplatin were administered. Among the 50 patients, 43 (86.0% received ≥6 cycles of nimotuzumab (median 7 cycles, range 2–14 cycles and 29 (58.0% received two cycles of CCT with docetaxel plus nedaplatin. Results: With a median follow-up of 28.0 months, the 2-year progression-free survival (PFS and overall survival were 83.29% (95% confidence interval [CI]: 67.93%–91.72% and 97.67% (95% CI: 84.62%–99.67%, respectively. Both univariate and multivariate analyses revealed that cycles of nimotuzumab were significantly associated with PFS. Patients who received ≥6 cycles of nimotuzumab showed a better PFS than those receiving <6 cycles (P=0.006, whereas the addition of CCT failed to improve PFS. Oral mucositis was the most common adverse event, which was recorded as grade 3–4 in 18 (36.0% patients. Besides, two (4.0% patients experienced

  10. Effects of Improving Primary Health Care Workers' Knowledge About Public Health Services in Rural China: A Comparative Study of Blended Learning and Pure E-Learning.

    Science.gov (United States)

    Zhan, Xingxin; Zhang, Zhixia; Sun, Fang; Liu, Qian; Peng, Weijun; Zhang, Heng; Yan, Weirong

    2017-05-01

    rural China, a blended-learning approach to BPHS training could result in a higher knowledge achievement and satisfaction level compared with a pure e-learning approach. The findings of the study will contribute knowledge to improve the competency of PHCWs in similar settings. ©Xingxin Zhan, Zhixia Zhang, Fang Sun, Qian Liu, Weijun Peng, Heng Zhang, Weirong Yan. Originally published in the Journal of Medical Internet Research (http://www.jmir.org), 01.05.2017.

  11. Comprehensive analysis of gene-expression profile in chronic obstructive pulmonary disease

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    Wei L

    2015-06-01

    Full Text Available Lei Wei,1,* Dong Xu,2,* Yechang Qian,1 Guoyi Huang,1 Wei Ma,1 Fangying Liu,1 Yanhua Shen,1 Zhongfu Wang,1 Li Li,1 Shanfang Zhang,1 Yafang Chen1 1Department of Respiratory Disease, Baoshan District Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai, 2Medical College of Soochow University, Suzhou, People's Republic of China *These authors contributed equally to this work Objective: To investigate the gene-expression profile of chronic obstructive pulmonary disease (COPD patients and explore the possible therapeutic targets. Methods: The microarray raw dataset GSE29133, including three COPD samples and three normal samples, was obtained from Gene Expression Omnibus. After data preprocessing with the Affy package, Student’s t-test was employed to identify the differentially expressed genes (DEGs. The up- and downregulated DEGs were then pooled for gene-ontology and pathway-enrichment analyses using the Database for Annotation, Visualization and Integrated Discovery (DAVID. The upstream regulatory elements of these DEGs were also explored by using Whole-Genome rVISTA. Furthermore, we constructed a protein–protein interaction (PPI network for DEGs. The surfactant protein D (SP-D serum level and HLA-A gene frequency in COPD patients and healthy controls were also measured by enzyme-linked immunosorbent assay (ELISA and real-time polymerase chain reaction, respectively. Results: A total of 39 up- and 15 downregulated DEGs were screened. Most of the upregulated genes were involved in the immune response process, while the downregulated genes were involved in the steroid metabolic process. Moreover, we also found that HLA-A has the highest degree in the PPI network. The SP-D serum level and HLA-A gene frequency in COPD patients were significantly higher than those in healthy controls (13.62±2.09 ng/mL vs 10.28±2.86 ng/mL; 62.5% vs 12.5%; P<0.05. Conclusion: Our results may help further the understanding of the mechanisms of

  12. Cancer survivors’ perspectives and experience on western medicine and traditional Chinese medicine treatment and rehabilitation: a qualitative study

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    Wang JW

    2014-12-01

    Full Text Available Ji-Wei Wang,1 Zhi-Qi Yang,1 Cong Liu,1 Si-Jia Chen,1 Qian Shen,1 Tian-Rui Zhang,1 Nancy S Partike,2 Zheng-Ping Yuan,3 Jin-Ming Yu1 1School of Public Health, Key Laboratory of Public Health Safety, Fudan University, Shanghai, People’s Republic of China; 2School of Public Health, University of Hawaii at Manoa, Honolulu, HI, USA; 3Shanghai Cancer Rehabilitation Club, Shanghai, People’s Republic of China Background: In the People’s Republic of China, both western medicine (WM and traditional Chinese medicine (TCM are the main treatment and rehabilitation options for cancer patients. This study aimed to explore cancer survivors’ perspectives and experience of treatment and rehabilitation, in order to promote patient-centered activities of treatment and rehabilitation.Methods: Using a qualitative research approach, 68 cancer survivors were recruited from eight community cancer rehabilitation organizations in Shanghai, People’s Republic of China. Eight focus group interviews were conducted. All these interviews were transcribed verbatim, and the data were analyzed by theme analysis.Results: WM was the main choice in treatment phase though study participants noted more side effects. TCM was primarily used in the recovery phase. The lack of communication between doctors and cancer patients appears to affect treatment adherence and impair the doctor–patient relationship. WM was expensive for diagnostic procedures and treatment, while the cumulative costs of frequent use of TCM in the long rehabilitation period were also high. Both treatment options created significant perceived economic burden on patients. Conflicting information about dietary supplements tended to make cancer survivors confused.Conclusion: Improving the communication between doctors and cancer patients helps to ameliorate cancer patient adherence and the effect of treatments. It is essential to educate cancer patients about the effect and cost of both WM and traditional TCM

  13. Correlation of primary middle and distal esophageal cancers motion with surrounding tissues using four-dimensional computed tomography

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    Wang W

    2016-06-01

    Full Text Available Wei Wang,1 Jianbin Li,1 Yingjie Zhang,1 Qian Shao,1 Min Xu,1 Bing Guo,1 Dongping Shang2 1Department of Radiation Oncology, 2Department of Big Bore CT Room, Shandong Cancer Hospital Affiliated to Shandong University, Shandong Academy of Medical Sciences, Jinan, Shandong, People’s Republic of China Purpose: To investigate the correlation of gross tumor volume (GTV motion with the structure of interest (SOI motion and volume variation for middle and distal esophageal cancers using four-dimensional computed tomography (4DCT.Patients and methods: Thirty-three patients with middle or distal esophageal carcinoma underwent 4DCT simulation scan during free breathing. All image sets were registered with 0% phase, and the GTV, apex of diaphragm, lung, and heart were delineated on each phase of the 4DCT data. The position of GTV and SOI was identified in all 4DCT phases, and the volume of lung and heart was also achieved. The phase relationship between the GTV and SOI was estimated through Pearson’s correlation test.Results: The mean peak-to-peak displacement of all primary tumors in the lateral (LR, anteroposterior (AP, and superoinferior (SI directions was 0.13 cm, 0.20 cm, and 0.30 cm, respectively. The SI peak-to-peak motion of the GTV was defined as the greatest magnitude of motion. The displacement of GTV correlated well with heart in three dimensions and significantly associated with bilateral lung in LR and SI directions. A significant correlation was found between the GTV and apex of the diaphragm in SI direction (rleft=0.918 and rright=0.928. A significant inverse correlation was found between GTV motion and varying lung volume, but the correlation was not significant with heart (rLR=–0.530, rAP=–0.531, and rSI=–0.588 during respiratory cycle.Conclusion: For middle and distal esophageal cancers, GTV should expand asymmetric internal margins. The primary tumor motion has quite good correlation with diaphragm, heart, and lung. Keywords

  14. Self-assembled nanoparticles based on the c(RGDfk peptide for the delivery of siRNA targeting the VEGFR2 gene for tumor therapy

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    Liu L

    2014-07-01

    Full Text Available Li Liu,1 Xiaoxia Liu,1 Qian Xu,1 Ping Wu,2 Xialin Zuo,3 Jingjing Zhang,1 Houliang Deng,1 Zhuomin Wu,1 Aimin Ji1 1Department of Pharmacy, Zhujiang Hospital, Southern Medical University, Guangzhou, People’s Republic of China; 2Department of Pharmacy, Chengdu Integrated TCM & Western Medicine Hospital, Chengdu, People’s Republic of China; 3Institute of Neurosciences and the Second Affiliated Hospital of Guangzhou Medical University, Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and the Ministry of Education of China, Guangzhou, People’s Republic of China Abstract: The clinical application of small interfering RNA (siRNA has been restricted by their poor intracellular uptake, low serum stability, and inability to target specific cells. During the last several decades, a great deal of effort has been devoted to exploring materials for siRNA delivery. In this study, biodegradable, tumor-targeted, self-assembled peptide nanoparticles consisting of cyclo(Arg–Gly–Asp–d–Phe–Lys-8–amino–3,6–dioxaoctanoic acid–β–maleimidopropionic acid (hereafter referred to as RPM were found to be an effective siRNA carrier both in vitro and in vivo. The nanoparticles were characterized based on transmission electron microscopy, circular dichroism spectra, and dynamic light scattering. In vitro analyses showed that the RPM/VEGFR2-siRNA exhibited negligible cytotoxicity and induced effective gene silencing. Delivery of the RPM/VEGFR2 (zebrafish-siRNA into zebrafish embryos resulted in inhibition of neovascularization. Administration of RPM/VEGFR2 (mouse-siRNA to tumor-bearing nude mice led to a significant inhibition of tumor growth, a marked reduction of vessels, and a downregulation of VEGFR2 (messenger RNA and protein in tumor tissue. Furthermore, the levels of IFN-α, IFN-γ, IL-12, and IL-6 in mouse serum, assayed via enzyme-linked immunosorbent assay, did not indicate any immunogenicity of the RPM/VEGFR2

  15. Stereotactic body radiation therapy for patients with recurrent pancreatic adenocarcinoma at the abdominal lymph nodes or postoperative stump including pancreatic stump and other stump

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    Zeng XL

    2016-06-01

    Full Text Available Xian-Liang Zeng,* Huan-Huan Wang,* Mao-Bin Meng, Zhi-Qiang Wu, Yong-Chun Song, Hong-Qing Zhuang, Dong Qian, Feng-Tong Li, Lu-Jun Zhao, Zhi-Yong Yuan, Ping Wang Department of Radiation Oncology, Tianjin’s Clinical Research Center for Cancer and Key Laboratory of Cancer Prevention and Therapy, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin, People’s Republic of China *These authors contributed equally to this work Background and aim: The aim of this study is to evaluate the efficacy and safety of stereotactic body radiation therapy (SBRT using CyberKnife in the treatment of patients with recurrent pancreatic adenocarcinoma at the abdominal lymph node or stump after surgery. Patients and methods: Between October 1, 2006 and May 1, 2015, patients with recurrent pancreatic adenocarcinoma at the abdominal lymph node or stump after surgery were enrolled and treated with SBRT at our hospital. The primary end point was local control rate after SBRT. Secondary end points were overall survival, time to symptom alleviation, and toxicity, assessed using the Common Terminology Criteria for Adverse Events version 4.0. Results: Twenty-four patients with 24 lesions (17 abdominal lymph nodes and seven stumps were treated with SBRT, of which five patients presented with abdominal lymph nodes and synchronous metastases in the liver and lung. The 6-, 12-, and 24-month actuarial local control rates were 95.2%, 83.8%, and 62.1%, respectively. For the entire cohort, the median overall survival from diagnosis and SBRT was 28.9 and 12.2 months, respectively. Symptom alleviation was observed in eleven of 14 patients (78.6% within a median of 8 days (range, 1–14 days after SBRT. Nine patients (37.5% experienced Common Terminology Criteria for Adverse Events version 4.0 grade 1–2 acute toxicities; one patient experienced grade 3 acute toxicity due to thrombocytopenia. Conclusion: SBRT is a safe and

  16. Quantum dots immunofluorescence histochemical detection of EGFR gene mutations in the non-small cell lung cancers using mutation-specific antibodies

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    Qu YG

    2014-12-01

    Full Text Available Yan-Gang Qu,1 Qian Zhang,2 Qi Pan,3 Xian-Da Zhao,4 Yan-Hua Huang,2 Fu-Chun Chen,3 Hong-Lei Chen41Department of Pathology, The Central Hospital of Enshi Autonomous Prefecture, Enshi, 2Department of Molecular Pathology, Wuhan Nano Tumor Diagnosis Engineering Research Center, Wuhan, Hubei, People’s Republic of China; 3Department of Thoracosurgery, Traditional Chinese Medical Hospital of Wenling, Wenling, Zhejiang, People’s Republic of China; 4Department of Pathology, School of Basic Medical Science, Wuhan University, Wuhan, Hubei, People’s Republic of ChinaBackground: Epidermal growth factor receptor (EGFR mutation status plays an important role in therapeutic decision making for non-small cell lung cancer (NSCLC patients. Since EGFR mutation-specific antibodies (E746-A750del and L858R have been developed, EGFR mutation detection by immunohistochemistry (IHC is a suitable screening test. On this basis, we want to establish a new screening test, quantum dots immunofluorescence histochemistry (QDs-IHC, to assess EGFR gene mutation in NSCLC tissues, and we compared it to traditional IHC and amplification refractory mutation system (ARMS.Materials and methods: EGFR gene mutations were detected by QDs-IHC, IHC, and ADx-ARMS in 65 cases of NSCLC composed of 55 formalin-fixed, paraffin-embedded specimens and ten pleural effusion cell blocks, including 13 squamous cell carcinomas, two adenosquamous carcinomas, and 50 adenocarcinomas.Results: Positive rates of EGFR gene mutations detected by QDs-IHC, IHC, and ADx-ARMS were 40.0%, 36.9%, and 46.2%, respectively, in 65 cases of NSCLC patients. The sensitivity of QDs-IHC when detecting EGFR mutations, as compared to ADx-ARMS, was 86.7% (26/30; the specificity for both antibodies was 100.0% (26/26. IHC sensitivity was 80.0% (24/30 and the specificity was 92.31% (24/26. When detecting EGFR mutations, QDs-IHC and ADx-ARMS had perfect consistency (κ=0.882; P<0.01. Excellent agreement was observed

  17. TRPA1 in the spinal dorsal horn is involved in post-inflammatory visceral hypersensitivity: in vivo study using TNBS-treated rat model

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    Li Q

    2016-12-01

    Full Text Available Qian Li,1,* Cheng-Hao Guo,2,* Mohammed Ali Chowdhury,1 Tao-Li Dai,1 Wei Han,1,3 1Department of Gastroenterology, Qilu Hospital of Shandong University, 2Department of Pathology, Medical School of Shandong University, 3Laboratory of Translational Gastroenterology, Shandong University, Qilu Hospital, Jinan, Shandong Province, People’s Republic of China *These authors contributed equally to this work Introduction: The transient receptor potential ankyrin-1 (TRPA1 channel, a pain transducer and amplifier, is drawing increasing attention in the field of visceral hypersensitivity, commonly seen in irritable bowel syndrome and inflammatory bowel disease. However, the role of TRPA1 in visceral nociception during post-inflammatory states is not well defined. Here, we explore the correlation between TRPA1 expression in the spinal dorsal horn (SDH and persistent post-inflammatory visceral hypersensitivity.Methods: We injected rats intracolonically with 2,4,6-trinitrobenzene sulfonic acid (TNBS or vehicle (n=12 per group. Post-inflammatory visceral hypersensitivity was assessed by recording the electromyographic activity of the external oblique muscle in response to colorectal distension. TRPA1 expression and distribution in the spinal cord and colon were examined by Western blotting and immunohistochemistry.Results: Animals exposed to TNBS had more abdominal contractions than vehicle-injected controls (P<0.05, which corresponded to a lower nociceptive threshold. Expression of TRPA1 in the SDH (especially in the substantia gelatinosa and the colon was significantly greater in the TNBS-treated group than in controls (P<0.05. In the SDH, the number of TRPA1-immunopositive neurons was 25.75±5.12 in the control group and 34.25±7.89 in the TNBS-treated group (P=0.023, and integrated optical density values of TRPA1 in the control and TNBS-treated groups were 14,544.63±6,525.54 and 22,532.75±7,608.11, respectively (P=0.041.Conclusion: Our results indicate

  18. Pharmacokinetics and bioavailability study of two ondansetron oral soluble film formulations in fasting healthy male Chinese volunteers

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    Zhu YB

    2015-08-01

    Full Text Available Yubing Zhu,1 Qian Zhang,1 Jianjun Zou,2 Meng Wan,1 Zheng Zhao,1 Junrong Zhu1 1Department of Pharmacy, 2Laboratory of Clinical Pharmacology, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu, People’s Republic of China Background: Ondansetron oral soluble film is designed to be applied on top of the tongue without requiring water to aid dissolution or swallowing, which is especially fitting for nausea and vomiting patients.Purpose: This study was conducted to compare the bioavailability of two 8 mg ondansetron oral soluble film formulations.Patients and methods: This randomized, open-label, two-period crossover study was performed under fasting conditions. A total of ten eligible subjects were randomly assigned at a 1:1 ratio to receive a single 8 mg dose of the test and reference ondansetron oral soluble film formulations, followed by a 1-week washout period and administration of the alternate formulation. The concentrations of ondansetron were assayed using an liquid chromatograph-mass spectrometer/mass spectrometer (LC-MS/MS method. For analysis of pharmacokinetic properties, including the peak concentration of Tmax (Cmax, AUC from time 0 (baseline to t hours (AUC0–t, and AUC from baseline to infinity (AUC0–∞, blood samples were obtained at intervals over the 24-hour period after studying drug administration. Tolerability was assessed by monitoring vital signs and laboratory tests (hematology, blood biochemistry, hepatic function, and urinalysis and by questioning subjects about adverse events.Results: The mean (standard derivation [SD] relative bioavailability was 96.5 (23.7%. The 90% confidence intervals (CIs for the log-transformed ratios of Cmax and AUC0–t were 84.71%–103.28% and 91.38%–108.60%, respectively (P>0.05. Similar results were found for the data without log-transformation. No statistically significant differences were found based on analysis of variance. No significant adverse events occurred

  19. Effect of Jian-Pi-Zhi-Dong Decoction on striatal glutamate and γ-aminobutyric acid levels detected using microdialysis in a rat model of Tourette syndrome

    Directory of Open Access Journals (Sweden)

    Zhang W

    2016-05-01

    Full Text Available Wen Zhang,1,* Li Wei,2,* Wenjing Yu,1 Xia Cui,1 Xiaofang Liu,2 Qian Wang,1 Sumei Wang2 1Department of Pediatrics, The Third Affiliated Hospital, 2Department of Pediatrics, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing, People’s Republic of China *These authors contributed equally to this work Background: Jian-Pi-Zhi-Dong Decoction (JPZDD is a dedicated treatment of Tourette syndrome (TS. The balance of neurotransmitters in the cortico-striato-pallido-thalamo-cortical network is crucial to the occurrence of TS and related to its severity. This study evaluated the effect of JPZDD on glutamate (Glu and γ-aminobutyric acid (GABA and their receptors in a TS rat model.Materials and methods: Rats were divided into four groups (n=12 each. TS was induced in three of the groups by injecting them with 3,3'-iminodipropionitrile for 7 consecutive days. Two model groups were treated with tiapride (Tia or JPZDD, while the control and the remaining model group were gavaged with saline. Behavior was assessed by stereotypic score and autonomic activity. Striatal Glu and GABA contents were detected using microdialysis. Expressions of N-methyl-D-aspartate receptor 1 and GABAA receptor (GABAAR were observed using Western blot and real-time polymerase chain reaction.Results: Tia and JPZDD groups had decreased stereotypy compared with model rats; however, the JPZDD group showed a larger decrease in stereotypy than the Tia group at a 4-week time point. In a spontaneous activity test, the total distance of the JPZDD and Tia groups was significantly decreased compared with the model group. The Glu levels of the model group were higher than the control group and decreased with Tia or JPZDD treatment. The GABA level was higher in the model group than the control group. Expressions of GABAAR protein in the model group were higher than in the control group. Treatment with Tia or JPZDD reduced the expression of GABAAR protein. In the case of the m

  20. Codelivery of curcumin and doxorubicin by MPEG-PCL results in improved efficacy of systemically administered chemotherapy in mice with lung cancer

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    Wang BL

    2013-09-01

    Full Text Available Bi-Lan Wang,1,* Yong-mei Shen,1,3,* Qiong-wen Zhang,1,* Yu-li Li,1 Min Luo,1 Ze Liu,1,2 Yan Li,1 Zhi-yong Qian,1 Xiang Gao,1 Hua-shan Shi1,2 1State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medicine School, Sichuan University, Chengdu, Sichuan, 2State Key Laboratory of Biotherapy and Department of Head and Neck Oncology, West China Hospital, West China Medical School, Sichuan University, Chengdu, Sichuan, 3Sichuang Haoyisheng Pharmacy, Chengdu, People’s Republic of China *These authors contributed equally to this work Abstract: Systemic administration of chemotherapy for cancer often has toxic side effects, limiting the doses that can be used in its treatment. In this study, we developed methoxy poly(ethylene glycol-poly(caprolactone (MPEG-PCL micelles loaded with curcumin and doxorubicin (Cur-Dox/MPEG-PCL that were tolerated by recipient mice and had enhanced antitumor effects and fewer side effects. It was shown that these Cur-Dox/MPEG-PCL micelles could release curcumin and doxorubicin slowly in vitro. The long circulation time of MPEG-PCL micelles and the slow rate of release of curcumin and doxorubicin in vivo may help to maintain plasma concentrations of active drug. We also demonstrated that Cur-Dox/MPEG-PCL had improved antitumor effects both in vivo and in vitro. The mechanism by which Cur-Dox/MPEG-PCL micelles inhibit lung cancer might involve increased apoptosis of tumor cells and inhibition of tumor angiogenesis. We found advantages using Cur-Dox/MPEG-PCL micelles in the treatment of cancer, with Cur-Dox/MPEG-PCL achieving better inhibition of LL/2 lung cancer growth in vivo and in vitro. Our study indicates that Cur-Dox/MPEG-PCL micelles may be an effective treatment strategy for cancer in the future. Keywords: methoxy poly(ethylene glycol, poly(caprolactone, curcumin, doxorubicin, micelles, cancer, treatment

  1. Anti-HER2 antibody and ScFvEGFR-conjugated antifouling magnetic iron oxide nanoparticles for targeting and magnetic resonance imaging of breast cancer

    Directory of Open Access Journals (Sweden)

    Chen H

    2013-10-01

    Full Text Available Hongwei Chen,1,* Liya Wang,1,2,* Qiqi Yu,1,2 Weiping Qian,3 Diana Tiwari,1 Hong Yi,4 Andrew Y Wang,5 Jing Huang,1,2 Lily Yang,3 Hui Mao1,2 1Department of Radiology and Imaging Sciences, 2Center for Systems Imaging, 3Department of Surgery, Emory University School of Medicine, 4Robert Apkarian Electron Microscopy Core, Emory University, Atlanta, GA, 5Ocean NanoTech LLC, Springdale, AK, USA *These authors contributed equally to this work Abstract: Antifouling magnetic iron oxide nanoparticles (IONPs coated with block copolymer poly(ethylene oxide-block-poly(γ-methacryloxypropyltrimethoxysilane (PEO-b-PγMPS were investigated for improving cell targeting by reducing nonspecific uptake. Conjugation of a HER2 antibody, Herceptin®, or a single chain fragment (ScFv of antibody against epidermal growth factor receptor (ScFvEGFR to PEO-b-PγMPS-coated IONPs resulted in HER2-targeted or EGFR-targeted IONPs (anti-HER2-IONPs or ScFvEGFR-IONPs. The anti-HER2-IONPs bound specifically to SK-BR-3, a HER2-overexpressing breast cancer cell line, but not to MDA-MB-231, a HER2-underexpressing cell line. On the other hand, the ScFvEGFR-IONPs showed strong reactivity with MDA-MB-231, an EGFR-positive human breast cancer cell line, but not with MDA-MB-453, an EGFR-negative human breast cancer cell line. Transmission electron microscopy revealed internalization of the receptor-targeted nanoparticles by the targeted cancer cells. In addition, both antibody-conjugated and non-antibody-conjugated IONPs showed reduced nonspecific uptake by RAW264.7 mouse macrophages in vitro. The developed IONPs showed a long blood circulation time (serum half-life 11.6 hours in mice and low accumulation in both the liver and spleen. At 24 hours after systemic administration of ScFvEGFR-IONPs into mice bearing EGFR-positive breast cancer 4T1 mouse mammary tumors, magnetic resonance imaging revealed signal reduction in the tumor as a result of the accumulation of the targeted IONPs

  2. Activation of PLC by an endogenous cytokine (GBP) in Drosophila S3 cells and its application as a model for studying inositol phosphate signalling through ITPK1.

    Science.gov (United States)

    Zhou, Yixing; Wu, Shilan; Wang, Huanchen; Hayakawa, Yoichi; Bird, Gary S; Shears, Stephen B

    2012-12-01

    Using immortalized [3H]inositol-labelled S3 cells, we demonstrated in the present study that various elements of the inositol phosphate signalling cascade are recruited by a Drosophila homologue from a cytokine family of so-called GBPs (growth-blocking peptides). HPLC analysis revealed that dGBP (Drosophila GBP) elevated Ins(1,4,5)P3 levels 9-fold. By using fluorescent Ca2+ probes, we determined that dGBP initially mobilized Ca2+ from intracellular pools; the ensuing depletion of intracellular Ca2+ stores by dGBP subsequently activated a Ca2+ entry pathway. The addition of dsRNA (double-stranded RNA) to knock down expression of the Drosophila Ins(1,4,5)P3 receptor almost completely eliminated mobilization of intracellular Ca2+ stores by dGBP. Taken together, the results of the present study describe a classical activation of PLC (phospholipase C) by dGBP. The peptide also promoted increases in the levels of other inositol phosphates with signalling credentials: Ins(1,3,4,5)P4, Ins(1,4,5,6)P4 and Ins(1,3,4,5,6)P5. These results greatly expand the regulatory repertoire of the dGBP family, and also characterize S3 cells as a model for studying the regulation of inositol phosphate metabolism and signalling by endogenous cell-surface receptors. We therefore created a cell-line (S3ITPK1) in which heterologous expression of human ITPK (inositol tetrakisphosphate kinase) was controlled by an inducible metallothionein promoter. We found that dGBP-stimulated S3ITPK1 cells did not synthesize Ins(3,4,5,6)P4, contradicting a hypothesis that the PLC-coupled phosphotransferase activity of ITPK1 [Ins(1,3,4,5,6)P5+Ins(1,3,4)P3→Ins(3,4,5,6)P4+Ins(1,3,4,6)P4] is driven solely by the laws of mass action [Chamberlain, Qian, Stiles, Cho, Jones, Lesley, Grabau, Shears and Spraggon (2007) J. Biol. Chem. 282, 28117-28125]. This conclusion represents a fundamental breach in our understanding of ITPK1 signalling.

  3. Holliday junction–recognizing protein promotes cell proliferation and correlates with unfavorable clinical outcome of hepatocellular carcinoma

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    Hu B

    2017-05-01

    Full Text Available Baohong Hu,1,2,* Qianli Wang,3,* Yueju Wang,4 Jian Chen,2 Peng Li,2 Mingyong Han1 1Department of Health Care Oncology, East District of Shandong Provincial Hospital of Shandong University, Jinan, 2Department of Medical Oncology, 3Department of Intensive Care Unit, Yantai Yuhuangding Hospital Affiliated to Qingdao University, Yantai, Shandong, 4Department of Geriatrics, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, People’s Republic of China *These authors contributed equally to this work Aim: To investigate the expression and clinical significance of Holliday junction–recognizing protein (HJURP in hepatocellular carcinoma (HCC. Methods: In this study, we detected the expression of HJURP protein in samples of 164 patients with HCC, and based on this, we divided the patients into two cohorts: high expression of HJURP and low expression of HJURP. We analyzed the correlation between HJURP expression and the clinicopathological factors using chi-square test. Survival significance of HJURP was defined by Kaplan–Meier method and log-rank test, and the independent prognostic factors were identified by Cox regression model. Using function assays of HCC cell lines, we investigated the influence of HJURP on the proliferation of HCC cells. Results: In our study, the proportion of patients with high HJURP expression was 25.6%, which was significantly associated with the tumor size and Barcelona clinic liver cancer stage. Univariate analysis confirmed that high HJURP expression was remarkably associated with poorer overall survival rates (P=0.003, as well as tumor number (P=0.016, tumor differentiation (P=0.047, TNM stage (P=0.005, and Barcelona clinic liver cancer stage (P=0.004. Multivariate analysis confirmed that high HJURP expression (P<0.001 acted as an independent prognostic risk factor of unfavorable prognosis. Real-time polymerase chain reaction analysis revealed that the expression of HJURP was significantly higher in

  4. Molecular dynamics simulations for the motion of evaporative droplets driven by thermal gradients along nanochannels

    KAUST Repository

    Wu, Congmin

    2013-04-04

    For a one-component fluid on a solid substrate, a thermal singularity may occur at the contact line where the liquid-vapor interface intersects the solid surface. Physically, the liquid-vapor interface is almost isothermal at the liquid-vapor coexistence temperature in one-component fluids while the solid surface is almost isothermal for solids of high thermal conductivity. Therefore, a temperature discontinuity is formed if the two isothermal interfaces are of different temperatures and intersect at the contact line. This leads to the so-called thermal singularity. The localized hydrodynamics involving evaporation/condensation near the contact line leads to a contact angle depending on the underlying substrate temperature. This dependence has been shown to lead to the motion of liquid droplets on solid substrates with thermal gradients (Xu and Qian 2012 Phys. Rev. E 85 061603). In the present work, we carry out molecular dynamics (MD) simulations as numerical experiments to further confirm the predictions made from our previous continuum hydrodynamic modeling and simulations, which are actually semi-quantitatively accurate down to the small length scales in the problem. Using MD simulations, we investigate the motion of evaporative droplets in one-component Lennard-Jones fluids confined in nanochannels with thermal gradients. The droplet is found to migrate in the direction of decreasing temperature of solid walls, with a migration velocity linearly proportional to the temperature gradient. This agrees with the prediction of our continuum model. We then measure the effect of droplet size on the droplet motion. It is found that the droplet mobility is inversely proportional to a dimensionless coefficient associated with the total rate of dissipation due to droplet movement. Our results show that this coefficient is of order unity and increases with the droplet size for the small droplets (∼10 nm) simulated in the present work. These findings are in semi

  5. Effects of HGF gene polymorphisms and protein expression on transhepatic arterial chemotherapeutic embolism efficacy and prognosis in patients with primary liver cancer

    Directory of Open Access Journals (Sweden)

    Chen HY

    2017-02-01

    Full Text Available Hai-Yong Chen,1,2 Yao-Min Chen,3 Jian Wu,1,2 Fu-Chun Yang,1,2 Zhen Lv,1,2 Yi-Gang Qian,1,2 Shu-Sen Zheng1,2 1Department of Surgery, Division of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital, Zhejiang University, 2Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, 3Department of Breast Surgery, The First Affiliated Hospital, Zhejiang University, Hangzhou, Zhejiang, People’s Republic of China Objective: To investigate the correlations of two hepatocyte growth factor (HGF gene polymorphisms (rs5745652 and rs2074725 and their protein expression levels with the efficacy of transhepatic arterial chemotherapeutic embolism (TACE and prognosis in patients with primary liver cancer (PLC. Methods: From March 2011 to June 2012, 109 PLC patients (the case group who chose TACE as primary treatment and 80 healthy people (the control group who had undergone physical examination in The First Affiliated Hospital, Zhejiang University were selected during the same period. Gene polymorphisms of HGF rs5745652 and HGF rs2074725 were detected. Serum HGF level, treating efficacy, survival quality, and 3-year survival rate for PLC patients who received TACE were observed. Results: There were significant differences in genotype and allele frequencies of HGF rs5745652 and HGF rs2074725, between the case and control groups (all P<0.05. Compared with CT+TT genotype of HGF rs5745652, patients carrying CC genotype had lower serum HGF levels, higher efficacy, better survival quality, and prolonged 3-year survival rate (all P<0.05. In rs2074725, patients carrying CA+AA genotype had lower serum HGF levels, higher efficacy, better survival quality, and prolonged 3-year survival rate compared with patients carrying rs2074725 CC genotype (all P<0.05. Gene polymorphisms of HGF rs5745652 and HGF rs2074725, tumor size, and Barcelona Clinic Liver Cancer stage were independent prognostic factors for PLC (P<0.05. Conclusion: Our

  6. WE-AB-207A-01: BEST IN PHYSICS (IMAGING): High-Resolution Cone-Beam CT of the Extremities and Cancellous Bone Architecture with a CMOS Detector

    Energy Technology Data Exchange (ETDEWEB)

    Cao, Q; Brehler, M; Sisniega, A; Marinetto, E; Stayman, J; Siewerdsen, J; Zbijewski, W [Johns Hopkins University, Baltimore, MD (United States); Zyazin, A; Peters, I [Teledyne DALSA, Eindhoven (Netherlands); Yorkston, J [Carestream Health, Inc, Penfield, NY (United States)

    2016-06-15

    -frequency imaging tasks through adoption of the CMOS detector and small FS x-ray source, motivating the use of these components in a new system for quantitative in-vivo imaging of trabecular bone. Financial Support: US NIH grant R01EB018896. Qian Cao is a Howard Hughes Medical Institute International Student Research Fellow. Disclosures: W Zbijewski, J Siewerdsen and A Sisniega receive research funding from Carestream Health.

  7. Electrospinning of calcium phosphate-poly(D,L-lactic acid nanofibers for sustained release of water-soluble drug and fast mineralization

    Directory of Open Access Journals (Sweden)

    Fu QW

    2016-10-01

    Full Text Available Qi-Wei Fu,1,* Yun-Peng Zi,1,* Wei Xu,1 Rong Zhou,1 Zhu-Yun Cai,1 Wei-Jie Zheng,1 Feng Chen,2 Qi-Rong Qian1 1Department of Orthopedics, Changzheng Hospital, Second Military Medical University, 2State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai, People’s Republic of China *These authors contributed equally to this work Abstract: Calcium phosphate-based biomaterials have been well studied in biomedical fields due to their outstanding chemical and biological properties which are similar to the inorganic constituents in bone tissue. In this study, amorphous calcium phosphate (ACP nanoparticles were prepared by a precipitation method, and used for preparation of ACP-poly(D,L-lactic acid (ACP-PLA nanofibers and water-soluble drug-containing ACP-PLA nanofibers by electrospinning. Promoting the encapsulation efficiency of water-soluble drugs in electrospun hydrophobic polymer nanofibers is a common problem due to the incompatibility between the water-soluble drug molecules and hydrophobic polymers solution. Herein, we used a native biomolecule of lecithin as a biocompatible surfactant to overcome this problem, and successfully prepared water-soluble drug-containing ACP-PLA nanofibers. The lecithin and ACP nanoparticles played important roles in stabilizing water-soluble drug in the electrospinning composite solution. The electrospun drug-containing ACP-PLA nanofibers exhibited fast mineralization in simulated body fluid. The ACP nanoparticles played the key role of seeds in the process of mineralization. Furthermore, the drug-containing ACP-PLA nanofibers exhibited sustained drug release which simultaneously occurred with the in situ mineralization in simulated body fluid. The osteoblast-like (MG63 cells with spreading filopodia were well observed on the as-prepared nanofibrous mats after culturing for 24 hours, indicating a high cytocompatibility. Due

  8. Combination of TRAIL and actinomycin D liposomes enhances antitumor effect in non-small cell lung cancer

    Directory of Open Access Journals (Sweden)

    Guo LG

    2012-03-01

    Full Text Available Liangran Guo1,2,4, Li Fan1,2, Jinfeng Ren1,2, Zhiqing Pang1,2, Yulong Ren1,2, Jingwei Li1,2, Ziyi Wen1,3, Yong Qian1,2, Lin Zhang1,2, Hang Ma4, Xinguo Jiang1,2 1School of Pharmacy, Fudan University, Zhangheng Road, Shanghai, 2Key Laboratory of Smart Drug Delivery, Ministry of Education and PLA, Shanghai, 3School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, People's Republic of China; 4College of Pharmacy, University of Rhode Island, RI, USAAbstract: The intractability of non-small cell lung cancer (NSCLC to multimodality treatments plays a large part in its extremely poor prognosis. Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL is a promising cytokine for selective induction of apoptosis in cancer cells; however, many NSCLC cell lines are resistant to TRAIL-induced apoptosis. The therapeutic effect can be restored by treatments combining TRAIL with chemotherapeutic agents. Actinomycin D (ActD can sensitize NSCLC cells to TRAIL-induced apoptosis by upregulation of death receptor 4 (DR4 or 5 (DR5. However, the use of ActD has significant drawbacks due to the side effects that result from its nonspecific biodistribution in vivo. In addition, the short half-life of TRAIL in serum also limits the antitumor effect of treatments combining TRAIL and ActD. In this study, we designed a combination treatment of long-circulating TRAIL liposomes and ActD liposomes with the aim of resolving these problems. The combination of TRAIL liposomes and ActD liposomes had a synergistic cytotoxic effect against A-549 cells. The mechanism behind this combination treatment includes both increased expression of DR5 and caspase activation. Moreover, systemic administration of the combination of TRAIL liposomes and ActD liposomes suppressed both tumor formation and growth of established subcutaneous NSCLC xenografts in nude mice, inducing apoptosis without causing significant general toxicity. These results provide preclinical proof

  9. Hyperhomocysteinemia is an independent predictor of long-term clinical outcomes in Chinese octogenarians with acute coronary syndrome

    Directory of Open Access Journals (Sweden)

    Fu Z

    2015-09-01

    Full Text Available Zhenhong Fu,1,* Geng Qian,1,* Hao Xue,1,* Jun Guo,1 Lian Chen,1 Xia Yang,1 Mingzhi Shen,2 Wei Dong,1 Yundai Chen11Department of Cardiology, Chinese People’s Liberation Army General Hospital, Beijing, People’s Republic of China; 2Department of Cardiology, Hainai Branch of Chinese People’s Liberation Army General Hospital, Sanya, Hainan, People’s Republic of China*These authors contributed equally to this workObjective: To evaluate the prognostic value of homocysteine (Hcy in Chinese acute coronary syndrome (ACS octogenarians.Methods: The study cohort comprised 660 consecutive ACS octogenarians who underwent coronary angiography. We classified the patients into three groups according to Hcy tertiles. Kaplan–Meier method was performed for survival and major adverse cardiac events (MACE rates. Multivariate Cox regression was performed to identify mortality predictors. Receiver operating characteristic curve analysis was performed to predict the cutoff value of Hcy for all-cause mortality.Results: The follow-up period was 28 (inter-quartile range: 16–38 months. Diastolic blood pressure, ratios of male, renal failure and old myocardial infarction in high plasma level Hcy (H-Hcy group were higher than those in low (L-Hcy and middle (M-Hcy plasma level of Hcy groups (P<0.05. The Hcy level was positively correlated with uric acid level (r=0.211, P=0.001 and Cystatin C (Cys C level (r=0.212, P=0.001 and negatively correlated with estimated glomerular filtration rate (r=−0.148, P=0.018. For the long-term outcomes, the cumulative survival rate of H-Hcy group was significantly lower than that of L-Hcy and M-Hcy groups (P=0.006. All-cause mortality and MACE of H-Hcy group were higher than those of L-Hcy and M-Hcy group (P=0.0001, P=0.0008. Hcy is an independent predictor for long-term all-cause mortality (odds ratio =2.26, 95% CI=1.23–4.16, P=0.023 and MACE (odds ratio =1.91, 95% CI=1.03–3.51, P=0.039. Receiver operating characteristic

  10. Peripheral inflammation/immune indicators of chemosensitivity and prognosis in breast cancer patients treated with neoadjuvant chemotherapy

    Directory of Open Access Journals (Sweden)

    Qian Y

    2018-03-01

    Full Text Available Yi Qian,1,* Jing Tao,1,2,* Xiuqing Li,3 Hua Chen,1 Qi Lu,1 Junzhe Yang,1 Hong Pan,1 Cong Wang,4 Wenbin Zhou,1 Xiaoan Liu1 1Department of Breast Surgery, The First Affiliated Hospital with Nanjing Medical University, Nanjing, China; 2Department of General Surgery, Nanjing Pukou Hospital, Nanjing, China; 3Department of Pathology, Jiangsu Province Hospital of Traditional Chinese Medicine, Nanjing, China; 4Department of Pathology, The First Affiliated Hospital with Nanjing Medical University, Nanjing, China *These authors contributed equally to this work Introduction: Neoadjuvant chemotherapy (NAC has become a standard treatment for locally advanced breast cancer. The present study was designed to investigate the predictive value of different peripheral inflammation/immune biomarker responses to NAC and prognosis in breast cancer patients. Materials and methods: A total of 180 breast cancer patients treated with NAC in the First Affiliated Hospital with Nanjing Medical University between January 2008 and March 2015 were enrolled in the study. The associations between inflammation/immune indicators and pathological complete response (pCR were determined, and the prognostic value of inflammation/immune indicators was also evaluated. Results: In the univariate analysis, patients with a high pretreatment peripheral lymphocyte count (>2.06×109/L showed a higher pCR rate than those with a low lymphocyte count (23.9% vs 10.4%, P=0.023. The pCR rate of patients with a neutrophil: lymphocyte ratio ≤2.15 was significantly higher than that of patients with a high neutrophil: lymphocyte ratio (20% vs 7.8%; P=0.03. However, multivariate analysis revealed that only the high lymphocyte count was predictive for pCR (odds ratio: 4.375, 95% CI: 1.429–13.392, P=0.010. In the survival analysis, patients with a higher neutrophil count (>2.65×109/L were confirmed to have a shorter disease-free survival (hazard ratio: 4.322, 95% CI: 1.028–18.174, P=0.046, and the

  11. The clinical significances of the abnormal expressions of Piwil1 and Piwil2 in colonic adenoma and adenocarcinoma

    Directory of Open Access Journals (Sweden)

    Wang HL

    2015-05-01

    Full Text Available Hai-Ling Wang,1 Bei-Bei Chen,1 Xin-Guang Cao,1 Jin Wang,2 Xiu-Feng Hu,1 Xiao-Qian Mu,1 Xiao-Bing Chen1 1The Affiliated Cancer Hospital of Zhengzhou University, Henan Cancer Hospital, Zhengzhou, People’s Republic of China; 2The First Affiliated Hospital of Zhengzhou University, Zhengzhou, People’s Republic of China Objective: The objective of the present investigation was to study the clinical significances of the abnormal expressions of Piwil1 and Piwil2 protein in colonic adenoma and adenocarcinoma.Methods: This study had applied immunohistochemical method to detect 45 cases of tissues adjacent to carcinoma (distance to cancerous tissue was above 5 cm, 41 cases of colonic adenoma and 92 cases of colon cancer tissues, and their Piwil1 and Piwil2 protein expression levels.Analysis: The correlation of both expression and its relationship with clinicopathological features of colon cancer was analyzed.Results: Positive expression rates of Piwil1 in tissues adjacent to carcinoma, colonic adenoma, and colon cancer were 11.1% (5/45, 53.7% (22/41, and 80.4% (74/92, respectively; the expression rates increased, and the comparisons between each two groups were statistically significant (P<0.05. In each group, the positive expression rates of Piwil2 were 24.4% (11/45 cases, 75.6% (31/41 cases, and 92.4% (85/92 cases; expression rates increased, and the comparisons between each two groups were statistically significant (P<0.05. Piwil1 expression and the correlation of the degree of differentiation, TNM stage, and lymph node metastasis were statistically significant (P<0.05. Piwil2 expression and the correlation of the degree of differentiation, tumor node metastasis (TNM stage, and lymph node metastasis had no statistical significance (P>0.05. In colon cancer tissue, Piwil1 and Piwil2 expressions were positively correlated (r=0.262, P<0.05.Conclusion: The results showed that the abnormal expression of Piwil1 and Piwil2 might play an important role in

  12. Microstructures, mechanical, and biological properties of a novel Ti-6V-4V/zinc surface nanocomposite prepared by friction stir processing

    Directory of Open Access Journals (Sweden)

    Zhu C

    2018-03-01

    Full Text Available Chenyuan Zhu,1,2,* Yuting Lv,3,4,* Chao Qian,1,2,* Zihao Ding,3,5 Ting Jiao,1,2 Xiaoyu Gu,1,2 Eryi Lu,6 Liqiang Wang,3 Fuqiang Zhang1,2 1Department of Prosthodontics, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, 2Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, National Clinical Research Center of Stomatology, 3State Key Laboratory of Metal Matrix Composites, Shanghai Jiao Tong University, Shanghai, 4College of Mechanical and Electronic Engineering, Shandong University of Science and Technology, Qingdao, People’s Republic of China; 5Department of Materials Science and Engineering, Carnegie Mellon University, Pittsburgh, PA, USA; 6Department of Stomatology, Ren Ji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People’s Republic of China *These authors contributed equally to this work Background: The interaction between the material and the organism affects the survival rate of the orthopedic or dental implant in vivo. Friction stir processing (FSP is considered a new solid-state processing technology for surface modification. Purpose: This study aims to strengthen the surface mechanical properties and promote the osteogenic capacity of the biomaterial by constructing a Ti-6Al-4V (TC4/zinc (Zn surface nanocomposites through FSP. Methods: FSP was used to modify the surface of TC4. The microstructures and mechanical properties were analyzed by scanning electron microscopy, transmission electron microscopy, nanoindentation and Vickers hardness. The biological properties of the modified surface were evaluated by the in vitro and in vivo study. Results: The results showed that nanocrystalline and numerous β regions, grain boundary a phase, coarser acicular α phase and finer acicular martensite α' appeared because of the severe plastic deformation caused by FSP, resulting in a decreased elastic modulus and an increased surface hardness. With the addition of

  13. Porous Se@SiO2 nanospheres treated paraquat-induced acute lung injury by resisting oxidative stress

    Directory of Open Access Journals (Sweden)

    Zhu Y

    2017-09-01

    Full Text Available Yong Zhu,1,* Guoying Deng,2,* Anqi Ji,2 Jiayi Yao,1 Xiaoxiao Meng,1 Jinfeng Wang,1 Qian Wang,2 Qiugen Wang,2 Ruilan Wang1 1Department of Critical Care Medicine, Shanghai General Hospital, Shanghai Jiaotong University, School of Medicine, 2Trauma Center, Shanghai General Hospital, Shanghai Jiaotong University, School of Medicine, Shanghai, China *These authors contributed equally to this work Abstract: Acute paraquat (PQ poisoning is one of the most common forms of pesticide poisoning. Oxidative stress and inflammation are thought to be important mechanisms in PQ-induced acute lung injury (ALI. Selenium (Se can scavenge intracellular free radicals directly or indirectly. In this study, we investigated whether porous Se@SiO2 nanospheres could alleviate oxidative stress and inflammation in PQ-induced ALI. Male Sprague Dawley rats and RLE-6TN cells were used in this study. Rats were categorized into 3 groups: control (n=6, PQ (n=18, and PQ + Se@SiO2 (n=18. The PQ and PQ + Se@SiO2 groups were randomly and evenly divided into 3 sub-groups according to different time points (24, 48 and 72 h after PQ treatment. Porous Se@SiO2 nanospheres 1 mg/kg (in the PQ + Se@SiO2 group were administered via intraperitoneal injection every 24 h. Expression levels of reduced glutathione, malondialdehyde, superoxide dismutase, reactive oxygen species (ROS, nuclear factor-κB (NF-κB, phosphorylated NF-κB (p-NF-κB, tumor necrosis factor-α and interleukin-1β were detected, and a histological analysis of rat lung tissues was performed. The results showed that the levels of ROS, malondialdehyde, NF-κB, p-NF-κB, tumor necrosis factor-α and interleukin-1β were markedly increased after PQ treatment. Glutathione and superoxide dismutase levels were reduced. However, treatment with porous Se@SiO2 nanospheres markedly alleviated PQ-induced oxidative stress and inflammation. Additionally, the results from histological examinations and wet-to-dry weight ratios of rat lung

  14. Aflatoxins, hydroxylated metabolites, and aflatoxicol from breast muscle of laying hens.

    Science.gov (United States)

    Díaz-Zaragoza, M; Carvajal-Moreno, M; Méndez-Ramírez, I; Chilpa-Galván, N C; Avila-González, E; Flores-Ortiz, C M

    2014-12-01

    Aflatoxins (AF) are toxic fungal secondary metabolites that are pathological to animals and humans. This study identified and quantified AF (AFB(1), AFB(2), AFG(1), AFG(2)) and their hydroxylated metabolites (AFM(1), AFM(2), AFP(1)) and aflatoxicol (AFL) from laying hen breast muscles. Aflatoxins pass from cereal feed to the laying hen tissues, causing economic losses, and from there to humans. To detect the passage of AF from feed to hen breast muscle tissues, an experiment that included 25 Hy-Line W36 121-wk-old hens was performed for 8 d. Hens in individual cages were distributed into 3 groups: a control group, with feed free of AFB(1), and 2 experimental groups, with feed spiked with 2 AFB(1) dosages: 30 µg·kg(-1) (low) or 500 µg·kg(-1) (high). The daily feed consumption per hen was recorded and afterward hens were euthanized and breast muscles were collected, weighed, and dried individually. Aflatoxins were extracted by 2 chemical methods and quantified by HPLC. Both methods were validated by lineality (calibration curves), recovery percentage (>80%), limit of detection, and limit of quantification. The AF (µg·kg(-1)) averages recovered in control breast muscles were as follows: AFB(1) (18); AFG(1), AFM(2), and AFL (0); AFG(2) (1.3); AFM(1) (52), and AFP1 (79). Hens fed with feed spiked with 30 µg·kg(-1) of AFB(1) had AFG(1) (16); AFG(2) (72); AFM(1) (0); AFM(2) (18); AFP(1) (145); and AFL (5 µg·kg(-1)). Hens with feed spiked with 500 µg·kg(-1) of AFB(1) had AFG(1) (512); AFG(2) (7); AFM(1) (4,775); AFM(2) (0); AFP(1) (661); and AFL (21 µg·kg(-1)). The best AF extraction method was Qian and Yang's method, modified by adding additional AF from both Supelclean LC18 SPE columns; its limit of detection (0.5 ng·mL(-1)) was lower compared with that of Koeltzow and Tanner, which was 1 ng·mL(-1). ©2014 Poultry Science Association Inc.

  15. Oleanolic acid induces mitochondrial-dependent apoptosis and G0/G1 phase arrest in gallbladder cancer cells

    Directory of Open Access Journals (Sweden)

    Li HF

    2015-06-01

    Full Text Available Huai-Feng Li,1–3,* Xu-An Wang,1–3,* Shan-Shan Xiang,1–3,* Yun-Ping Hu,1–3 Lin Jiang,1–3 Yi-Jun Shu,1–3 Mao-Lan Li,1–3 Xiang-Song Wu,1–3 Fei Zhang,1–3 Yuan-Yuan Ye,1–3 Hao Weng,1–3 Run-Fa Bao,1–3 Yang Cao,1–3 Wei Lu,1–3 Qian Dong,1–3 Ying-Bin Liu1–3 1Department of General Surgery, 2Laboratory of General Surgery, 3Institute of Biliary Tract Disease, Xinhua Hospital, Affiliated to Shanghai Jiao Tong University, School of Medicine, Shanghai, People’s Republic of China *These authors contributed equally to this work Abstract: Oleanolic acid (OA, a naturally occurring triterpenoid, exhibits potential antitumor activity in many tumor cell lines. Gallbladder carcinoma is the most common malignancy of the biliary tract, and is a highly aggressive tumor with an extremely poor prognosis. Unfortunately, the effects of OA on gallbladder carcinoma are unknown. In this study, we investigated the effects of OA on gallbladder cancer cells and the underlying mechanism. The results showed that OA inhibits proliferation of gallbladder cancer cells in a dose-dependent and time-dependent manner on MTT and colony formation assay. A flow cytometry assay revealed apoptosis and G0/G1 phase arrest in GBC-SD and NOZ cells. Western blot analysis and a mitochondrial membrane potential assay demonstrated that OA functions through the mitochondrial apoptosis pathway. Moreover, this drug inhibited tumor growth in nude mice carrying subcutaneous NOZ tumor xenografts. These data suggest that OA inhibits proliferation of gallbladder cancer cells by regulating apoptosis and the cell cycle process. Thus, OA may be a promising drug for adjuvant chemotherapy in gallbladder carcinoma. Keywords: oleanolic acid, gallbladder carcinoma, apoptosis, cell cycle arrest, mitochondrial pathway

  16. Functionalized milk-protein-coated magnetic nanoparticles for MRI-monitored targeted therapy of pancreatic cancer

    Directory of Open Access Journals (Sweden)

    Huang J

    2016-07-01

    Full Text Available Jing Huang,1,2 Weiping Qian,3 Liya Wang,1,2 Hui Wu,1 Hongyu Zhou,3 Andrew Yongqiang Wang,4 Hongbo Chen,5 Lily Yang,3 Hui Mao1,2 1Department of Radiology and Imaging Sciences, 2Center for Systems Imaging, 3Department of Surgery, Emory University School of Medicine, Atlanta, GA, USA; 4Ocean Nanotech LLC, Springdale, AR, USA; 5School of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin, Guangxi, People’s Republic of China Abstract: Engineered nanocarriers have emerged as a promising platform for cancer therapy. However, the therapeutic efficacy is limited by low drug loading efficiency, poor passive targeting to tumors, and severe systemic side effects. Herein, we report a new class of nanoconstructs based on milk protein (casein-coated magnetic iron oxide (CNIO nanoparticles for targeted and image-guided pancreatic cancer treatment. The tumor-targeting amino-terminal fragment (ATF of urokinase plasminogen activator and the antitumor drug cisplatin (CDDP were engineered on this nanoplatform. High drug loading (~25 wt% and sustained release at physiological conditions were achieved through the exchange and encapsulation strategy. These ATF-CNIO-CDDP nanoparticles demonstrated actively targeted delivery of CDDP to orthotopic pancreatic tumors in mice. The effective accumulation and distribution of ATF-CNIO-CDDP was evidenced by magnetic resonance imaging, based on the T2-weighted contrast resulting from the specific accumulation of ATF-CNIO-CDDP in the tumor. Actively targeted delivery of ATF-CNIO-CDDP led to improved therapeutic efficacy in comparison with free CDDP and nontargeted CNIO-CDDP treatment. Meanwhile, less systemic side effects were observed in the nanocarrier-treated groups than that in the group treated with free CDDP. Hematoxylin and Eosin and Sirius Red staining of tumor sections revealed the possible disruption of stroma during the treatment with ATF-CNIO-CDDP. Overall, our results suggest that

  17. Association between pretreatment Glasgow prognostic score and gastric cancer survival and clinicopathological features: a meta-analysis

    Directory of Open Access Journals (Sweden)

    Zhang CX

    2016-06-01

    Full Text Available Chun-Xiao Zhang,* Shu-Yi Wang,* Shuang-Qian Chen, Shuai-Long Yang, Lu Wan, Bin Xiong Department of Oncology, Zhongnan Hospital of Wuhan University, Hubei Key Laboratory of Tumor Biological Behaviors and Hubei Cancer Clinical Study Center, Wuhan, Hubei, People’s Republic of China *These authors contributed equally to this work Background: Glasgow prognostic score (GPS is widely known as a systemic inflammatory-based marker. The relationship between pretreatment GPS and gastric cancer (GC survival and clinicopathological features remains controversial. The aim of the study was to conduct a meta-analysis of published studies to evaluate the association between pretreatment GPS and survival and clinicopathological features in GC patients. Methods: We searched PubMed, Embase, MEDLINE, and BioMed databases for relevant studies. Combined analyses were used to assess the association between pretreatment GPS and overall survival, disease-free survival, and clinicopathological parameters by Stata Version 12.0. Results: A total of 14 studies were included in this meta-analysis, including 5,579 GC patients. The results indicated that pretreatment high GPS (HGPS predicted poor overall survival (hazard ratio =1.51, 95% CI: 1.37–1.66, P<0.01 and disease-free survival (hazard ratio =1.45, 95% CI: 1.26–1.68, P<0.01 in GC patients. Pretreatment HGPS was also significantly associated with advanced tumor–node–metastasis stage (odds ratio [OR] =3.09, 95% CI: 2.11–4.53, P<0.01, lymph node metastasis (OR =4.60, 95% CI: 3.23–6.56, P<0.01, lymphatic invasion (OR =3.04, 95% CI: 2.00–4.62, P<0.01, and venous invasion (OR =3.56, 95% CI: 1.81–6.99, P<0.01. Conclusion: Our meta-analysis indicated that pretreatment HGPS could be a predicative factor of poor survival outcome and clinicopathological features for GC patients. Keywords: Glasgow prognostic score, gastric cancer, survival, clinicopathological feature

  18. Pri-let-7a-1 rs10739971 polymorphism is associated with gastric cancer prognosis and might affect mature let-7a expression

    Directory of Open Access Journals (Sweden)

    Li Y

    2016-07-01

    Full Text Available Ying Li, Qian Xu, Jingwei Liu, Caiyun He, Quan Yuan, Chengzhong Xing, Yuan Yuan Tumor Etiology and Screening Department of Cancer Institute and General Surgery, the First Affiliated Hospital of China Medical University, and Key Laboratory of Cancer Etiology and Prevention (China Medical University, Liaoning Provincial Education Department, Shenyang, People’s Republic of China Abstract: The relationship between the pri-let-7a-1 rs10739971 polymorphism and gastric cancer (GC risk has been reported. However, the role of this polymorphism in the prognosis of GC remains largely elusive. Sequenom MassARRAY platform method and the polymerase chain reaction (PCR-restriction fragment length polymorphism were used to investigate pri-let-7a-1 rs10739971 G→A in 334 GC patients. Real-time PCR detected expression of mature let-7a in serum and tissue. Patients with AA or GA+AA genotypes of the pri-let-7a-1 rs10739971 polymorphism demonstrated significantly longer survival time than those with the wild GG genotype. Stratified analysis indicated that survival time was significantly longer in women with AA or GA+AA genotypes and in Borrmann type I/II patients with GA heterozygote or GA+AA genotypes. AA genotype was more frequent in the lymphatic-metastasis-negative subgroup. Serum mature let-7a expression in healthy people with the GA heterozygote and the GA+AA genotype was higher than in those with the GG genotype, and the difference remained significant in the female healthy subgroup. Pri-let-7a-1 rs10739971 polymorphism might be a biomarker for GC prognosis, especially for female and Borrmann type I/II patients. The pri-let-7a-1 rs10739971 polymorphism might affect serum mature let-7a expression, and partly explain the mechanism of the relationship between the pri-let-7a-1 rs10739971 polymorphism and GC survival. Keywords: miRNA, let-7a, polymorphism, gastric cancer, prognosis, expression

  19. China's space development history: A comparison of the rocket and satellite sectors

    Science.gov (United States)

    Erickson, Andrew S.

    2014-10-01

    China is the most recent great power to emerge in aerospace. It has become the first developing nation to achieve some measure of aerospace production capability across the board. Outside the developed aerospace powers, only China has demonstrated competence concerning all aspects of a world-class aerospace industry: production of advanced rockets, satellites, and aircraft and of their supporting engineering, materials, and systems. As an emerging great power during the Cold War, China was still limited in resources, technology access, and capabilities. It thereby faced difficult choices and constraints. Yet it achieved increasing, though uneven, technological levels in different aerospace sub-sectors. Explaining this variance can elucidate challenges and opportunities confronting developing nations sharing limitations that previously constrained China. Rockets (missiles and space launch vehicles/SLVs) and satellites (military and civilian) were two areas of early achievement for China, and represent this article's two in-depth case studies. Initial import of American and Soviet knowledge and technology, coupled with national resources focused under centralized leadership, enabled China to master missiles and satellites ahead of other systems. Early in the Cold War, great power status hinged on atomic development. China devoted much of its limited technical resources to producing nuclear weapons in order to “prevent nuclear blackmail,” “break the superpowers' monopoly,” and thereby secure great power status. Beijing's second strategic priority was to develop reliable ballistic missiles to credibly deliver warheads, thereby supporting nuclear deterrence. Under Chairman Mao Zedong's direction and the guidance of the American-educated Dr. Qian Xuesen (H.S. Tsien), missile development became China's top aerospace priority. Satellites were also prioritized for military-strategic reasons and because they could not be purchased from abroad following the Sino

  20. Metformin inhibits proliferation and cytotoxicity and induces apoptosis via AMPK pathway in CD19-chimeric antigen receptor-modified T cells

    Directory of Open Access Journals (Sweden)

    Mu Q

    2018-04-01

    Full Text Available Qian Mu,1,2,* Miao Jiang,1,* Yuzhu Zhang,1 Fei Wu,1 Hui Li,1 Wen Zhang,1 Fang Wang,1 Jiang Liu,1 Liang Li,1 Dongshan Wang,3 Wenjuan Wang,1 Shiwu Li,1 Haibo Song,4 Dongqi Tang1 1Gene and Immunotherapy Center, The Second Hospital of Shandong University, Jinan, People’s Republic of China; 2Department of Endocrinology and Metabolism, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People’s Republic of China; 3Health Management Center, The Second Hospital of Shandong University, Jinan, People’s Republic of China; 4Central Research Laboratory, Zibo Maternal and Child Health Hospital, Affiliated to Shandong Academy of Medical Science, Zibo, People’s Republic of China *These authors contributed equally to this work Background: CD19-chimericantigen receptor (CAR modified T cells (CD19-CAR T cells have been well documented to possess potent anti-tumor properties against CD19-expressingleukemia cells. As a traditional medicine, metformin has been widely used to treat type II diabetes mellitus and more recently has become a candidate for the treatment of cancer. However, no report has revealed the direct effect of metformin on CD19-CAR T cell biological function and its underling mechanisms. Purpose: The purpose of this research was to explore the effect of metformin on CD19-CAR T cell biological function and the mechanisms involved. Methods: CD19-CAR T cells proliferation, apoptosis and cytotoxicity were mainly tested by CCK-8 assay, flow cytometry and ELISA. The detection of mechanism primarily used western blot. Bioluminescence imaging is the main application technology of animal studies. Results: In the current study, it was found that metformin inhibited CD19-CAR T cell proliferation and cytotoxicity and induced apoptosis. Furthermore, our study revealed that metformin activated AMPK and suppressed mTOR and HIF1α expression. By using an AMPK inhibitor, compound C, we demonstrated the crucial roles of AMPK in CD19

  1. Skeleton labeled 13C-carbon nanoparticles for the imaging and quantification in tumor drainage lymph nodes

    Directory of Open Access Journals (Sweden)

    P Xie

    2017-07-01

    Full Text Available Ping Xie,1 Qian Xin,2 Sheng-Tao Yang,3 Tiantian He,2,4 Yuanfang Huang,2 Guangfu Zeng,2,4 Maosheng Ran,2,5 Xiaohai Tang2 1State Key Laboratory of Oral Diseases, West China College of Stomatology, Sichuan University, Chengdu, 2Chongqing Lummy Pharmaceutical Co., Ltd, Chongqing, 3College of Chemistry & Environment Protection Engineering, Southwest University for Nationalities, 4College of Life Sciences, Sichuan Normal University, 5State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu, People’s Republic of China Abstract: Carbon nanoparticles (CNPs have been widely used in tumor drainage lymph node (TDLN imaging, drug delivery, photothermal therapy, and so on. However, during the theranostic applications, the accumulation efficiency of CNPs in target organs is unknown yet, which largely hinders the extension of CNPs into clinical uses. Herein, we prepared skeleton-labeled 13C-CNPs that had identical properties to commercial CNPs suspension injection (CNSI for the imaging and quantification in TDLN. 13C-CNPs were prepared by arc discharge method, followed by homogenization with polyvinylpyrrolidone. The size distribution and morphology of 13C-CNPs were nearly the same as those of CNSI under transmission electron microscope. The hydrodynamic radii of both 13C-CNPs and CNSI were similar, too. According to X-ray photoelectron spectroscopy and infrared spectroscopy analyses, the chemical compositions and chemical states of elements were also nearly identical for both labeled and commercial forms. The skeleton labeling of 13C was reflected by the shift of G-band toward lower frequency in Raman spectra. 13C-CNPs showed competitive performance in TDLN imaging, where the three lymph nodes (popliteal lymph node, common iliac artery lymph node, and paraaortic lymph node were stained black upon the injection into the hind extremity of mice. The direct quantification of 13C-CNPs indicated that 877 µg

  2. The outcomes of intra-aortic balloon pump usage in patients with acute myocardial infarction: a comprehensive meta-analysis of 33 clinical trials and 18,889 patients

    Directory of Open Access Journals (Sweden)

    Fan ZG

    2016-03-01

    Full Text Available Zhong-Guo Fan,1,* Xiao-Fei Gao,1,2,* Li-Wen Chen,1 Xiao-Bo Li,1,2 Ming-Xue Shao,1,2 Qian Ji,1 Hao Zhu,1 Yi-Zhi Ren,1 Shao-Liang Chen,1,2 Nai-Liang Tian1,2 1Department of Cardiology, Nanjing First Hospital, Nanjing Medical University, 2Department of Cardiology, Nanjing Heart Center, Nanjing, People’s Republic of China *These authors contributed equally to this work Background: The effects of intra-aortic balloon pump (IABP usage in patients with acute myocardial infarction remain controversial. This study sought to evaluate the outcomes of IABP usage in these patients.Methods: Medline, EMBASE, and other internet sources were searched for relevant clinical trials. The primary efficacy endpoints (in-hospital, midterm, and long-term mortality and secondary endpoints (reinfarction, recurrent ischemia, and new heart failure in the hospital as well as safety endpoints (severe bleeding requiring blood transfusion and stroke in-hospital were subsequently analyzed.Results: Thirty-three clinical trials involving 18,889 patients were identified. The risk of long-term mortality in patients suffering from acute myocardial infarction was significantly decreased following IABP use (odds ratio [OR] 0.66, 95% confidence interval [CI]: 0.48–0.91, P=0.010. Both in-hospital and midterm mortality did not differ significantly between the IABP use group and no IABP use group (in-hospital: OR 0.87, 95% CI: 0.59–1.28, P=0.479; midterm: OR 1.12, 95% CI: 0.53–2.38, P=0.768. IABP insertion was not associated with the risk reduction of reinfarction, recurrent ischemia, or new heart failure. However, IABP use increased the risk of severe bleeding requiring blood transfusion (OR 2.05, 95% CI: 1.29–3.25, P=0.002 and stroke (OR 1.71, 95% CI: 1.04–2.82, P=0.035. In the thrombolytic therapy and cardiogenic shock subgroups, reduced mortality rates following IABP use were observed.Conclusion: IABP insertion is associated with feasible benefits with respect to long

  3. Chitosan nanoparticle-based delivery of fused NKG2D–IL-21 gene suppresses colon cancer growth in mice

    Directory of Open Access Journals (Sweden)

    Tan L

    2017-04-01

    Full Text Available Lunmei Tan,1 Sen Han,2 Shizhen Ding,2 Weiming Xiao,3,4 Yanbing Ding,3 Li Qian,2,4 Chenming Wang,1,5 Weijuan Gong1–5 1Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, 2Department of Immunology, School of Medicine, 3Department of Gastroenterology, The Second Clinical Medical College, 4Department of Integrated Chinese and Western Medicine, School of Medicine, 5Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, People’s Republic of China Abstract: Nanoparticles can be loaded with exogenous DNA for the potential expression of cytokines with immune-stimulatory function. NKG2D identifies major histocompatibility complex class I chain-related protein in human and retinoic acid early induced transcript-1 in mouse, which acts as tumor-associated antigens. Biologic agents based on interleukin 21 (IL-21 have displayed antitumor activities through lymphocyte activation. The NKG2D–IL-21 fusion protein theoretically identifies tumor cells through NKG2D moiety and activates T cells through IL-21 moiety. In this study, double-gene fragments that encode the extracellular domains of NKG2D and IL-21 genes were connected and then inserted into the pcDNA3.1(– plasmid. PcDNA3.1–dsNKG2D–IL-21 plasmid nanoparticles based on chitosan were generated. Tumor cells pretransfected with dsNKG2D–IL-21 gene nanoparticles can activate natural killer (NK and CD8+ T cells in vitro. Serum IL-21 levels were enhanced in mice intramuscularly injected with the gene nanoparticles. DsNKG2D–IL-21 gene nanoparticles accumulated in tumor tissues after being intravenously injected for ~4–24 h. Treatment of dsNKG2D–IL-21 gene nanoparticles also retarded tumor growth and elongated the life span of tumor-bearing mice by activating NK and T cells in vivo. Thus, the dsNKG2D–IL-21 gene nanoparticles exerted efficient antitumor activities and would be potentially used for tumor therapy. Keywords: NKG2

  4. Translation Str A Comparison of ategies from the Perspective of Two Chinese Versions of The Importance%文化转向视角下的翻译策略研究——以王尔德The Importance of Being Earnest两中译本为例

    Institute of Scientific and Technical Information of China (English)

    施秋蕾

    2012-01-01

    The cultural turn of translation studies offers a brand-new perspective on both theory and practice, extending the scope from the linguistic level to the cultural level. It also makes the choice between translation strategies even more complicated. This paper analyses the different strategies adopted by Yu Guangzhong and Qianzhide when translating Oscar Wilde' s masterpiece The Importance of Being Earnest from the viewpoint of cultural turn. With the purpose of performability, Yu employs the domestication strategy, integrating the foreign culture into the target culture; while Qian focuses on the text itself and applies foreignization, keeping the foreign culture intact. It is suggested that purpose permitting, the translator should adopt the foreignization strategy more frequently in order to boost the exchange between two cuhures.%翻译的“文化转向”为翻译研究提供了一个全新视角,使之从语言层面扩展至文化层面,同时也使翻译策略的选择变得更为复杂。从文化转向的视角分析余光中与钱之德在翻译王尔德名剧弛The Importance of Being Earnest时的策略选择可知,余译以剧本的舞台表演性为目的,采取了归化策略,将异国文化融入目的语文化中;钱译不以表演性为目的,采取了异化策略,保留了异域文化特色。译者应在不违背翻译目的的前提下,尽可能多地采用异化策略,以促进原语文化和目的语文化的交流。

  5. Virtue Existential Career Model: A Dialectic and Integrative Approach Echoing Eastern Philosophy.

    Science.gov (United States)

    Liu, Shu-Hui; Hung, Jui-Ping; Peng, Hsin-I; Chang, Chia-Hui; Lu, Yi-Jen

    2016-01-01

    Our Virtue Existential Career (VEC) model aims at complementing western modernism and postmodernism career theories with eastern philosophy. With dialectical philosophy and virtue-practice derived from the Classic of Changes , the VEC theoretical foundation incorporates merits from Holland typology, Minnesota Theory of Work Adjustment, Social Cognitive Career Theory, Meaning Therapy, Narrative Approach Career Counseling, and Happenstance Learning Theory. While modernism considers a matched job as an ideal career vision and prefers rational strategies ( controlling and realizing ) to achieve job security; postmodernism prefers appreciating and adapting strategies toward openness and appreciates multiple possible selves and occupations, our model pursues a blending of security and openness via controlling-and-realizing and appreciating-and-adapting interwoven with each other in a dialectical and harmonious way. Our VEC counseling prototype aims at a secular goal of living on the earth with ways and harmony () and an ultimate end to spiral up to the wisdom of living up to the way of heaven () with mind and virtue (). A VEC counseling process of five major career strategies, metaphorical stories of qian and kun , and experiential activities are developed to deliver VEC concepts. The VEC model and prototype presented in this research is the product of an action research following Lewin's (1946) top-to-down model. Situated structure analyses were conducted to further investigate the adequacy of this version of VEC model and prototype. Data from two groups (one for stranded college graduates and the other for growing college students) revealed empirical supports. Y ang type of career praxes tends to induce actualization, which resulting in realistic goals and concrete action plans; yin type of career praxes tends to increase self-efficacy, which resulting in positive attitude toward current situatedness and future development. Acceptance and dialectic thinking often

  6. Virtue Existential Career Model: A Dialectic and Integrative Approach Echoing Eastern Philosophy

    Directory of Open Access Journals (Sweden)

    Shu-Hui Liu

    2016-11-01

    Full Text Available Our Virtue Existential Career (VEC model aims at complementing western modernism and postmodernism career theories with eastern philosophy. With dialectical philosophy and virtue-practice derived from the Classic of Changes, our VEC theoretical foundation incorporates merits from Holland typology, Minnesota Theory of Work Adjustment, Social Cognitive Career Theory, Meaning Therapy, Narrative Approach Career Counseling, and Happenstance Learning Theory. While modernism considers a matched job as an ideal career vision and prefers rational strategies (controlling and realizing to achieve job security; postmodernism prefers adapting and appreciating strategies toward openness and appreciates multiple possible selves and occupations, our VEC model pursues a blending of security and openness via controlling-and-realizing and appreciating-and-adapting interwoven with each other in a dialectical and harmonious way. Our VEC counseling prototype aims at a secular goal of living on the earth with ways and harmony (安身以法以和 and an ultimate end to spiral up to the wisdom of living up to the way of heaven (天道 with mind and virtue (立命以心以德. A VEC counseling process of five major career strategies, metaphorical stories of qian and kun, and experiential activities are developed to deliver VEC concepts. The VEC model and prototype presented in this research is the product of an action research following Lewin’s (1946 top-to-down model. Situated structure analyses were conducted to further investigate the adequacy of this version of VEC model and prototype. Data from two groups (one for stranded college graduates and the other for growing college students revealed empirical supports. Yang type of career praxes tend to induce actualization, which resulting in realistic goals and concrete action plans; yin type of career praxes tend to increase self-efficacy, which resulting in positive attitude toward current situatedness and future

  7. Enrichment of CD44 in basal-type breast cancer correlates with EMT, cancer stem cell gene profile, and prognosis

    Directory of Open Access Journals (Sweden)

    Xu HX

    2016-01-01

    Full Text Available Hanxiao Xu,1 Yijun Tian,1 Xun Yuan,1 Yu Liu,2 Hua Wu,1 Qian Liu,1 Gen Sheng Wu,3,4 Kongming Wu1 1Department of Oncology, 2Department of Geriatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People’s Republic of China; 3Department of Oncology, 4Department of Pathology, Karmanos Cancer Institute, Wayne State University School of Medicine, Detroit, MI, USA Abstract: Cluster of differentiation 44 (CD44 is a transmembrane glycoprotein that serves as the receptor for the extracellular matrix component hyaluronic acid. CD44 has been reported to play key roles in cell proliferation, motility, and survival, but its role in breast cancer remains controversial. In this study, we conducted a meta-analysis. A total of 23 published Gene Expression Omnibus databases were included to evaluate the association between CD44 mRNA expression and clinicopathological characteristics or prognosis of the patients with breast cancer. Our analysis revealed that CD44 expression was associated with clinicopathological features, including the histological grade, estrogen receptor status, progesterone receptor status, and human epidermal growth factor receptor-2 status. Higher levels of CD44 expression were observed in the basal subtype of breast cancer both at the mRNA and protein levels (odds ratio [OR] =2.08, 95% confidence interval [CI]: 1.72–2.52; OR =2.11, 95% CI: 1.67–2.68. Patients with CD44 overexpression exhibited significantly worse overall survival (hazard ratio =1.27; 95% CI: 1.04–1.55. Whole gene profile analysis revealed that CD44 expression was enriched in basal-type breast cancer and correlated with epithelial–mesenchymal transition and cancer stem cell gene profiles. In summary, our analyses indicated that CD44 potentially might be a prognostic marker for breast cancer and thus can serve as a therapeutic target for basal-type breast cancer. Keywords: breast cancer, CD44, survival prediction, meta

  8. Health-related quality of life in dialysis patients with constipation: a cross-sectional study

    Directory of Open Access Journals (Sweden)

    Zhang JS

    2013-06-01

    Full Text Available JiSheng Zhang,1,* CongYang Huang,1,* YanLi Li,1 Jun Chen,2 FangYuan Shen,1 Qiang Yao,3 JiaQi Qian,4 BeiYan Bao,1 XuPing Yao51Division of Nephrology, School of Medicine, Ningbo Urology and Nephrology Hospital, Ningbo University, Ningbo, Zhejiang, People's Republic of China; 2Blood Purification Center, School of Medicine, Ningbo Urology and Nephrology Hospital, Ningbo University, Ningbo, Zhejiang, People's Republic of China; 3Baxter Healthcare Pty Ltd, Shanghai, People's Republic of China; 4Division of Nephrology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China; 5Division of Urology, School of Medicine, Ningbo Urology and Nephrology Hospital, Ningbo University, Ningbo, Zhejiang, People's Republic of China *These authors contributed equally to this workObjectives: To evaluate differences in the health-related quality of life (HRQoL between patients with constipation receiving hemodialysis (HD and those receiving peritoneal dialysis (PD.Methods: In this cross-sectional study, 605 dialysis patients (478 HD cases and 127 PD cases; all patients were older than 18 years from our hospital were included. A questionnaire was used to evaluate their constipation statuses. The effect of constipation on HRQoL was assessed, using the Chinese version of the 12-item short-form (SF-12 general health survey. Karnofsky score, sociodemographic, and clinical data were also collected. We performed multiple logistic regression analysis to define independent risk factors for constipation and impaired HRQoL.Results: A total of 605 participants (326 men [53.9%] and 279 women [46.1%] were surveyed. The incidence of constipation was 71.7% in HD patients and 14.2% in PD patients. Dialysis patients with constipation had significantly lower mean SF-12 Physical Component Summary scale and Mental Component Summary scale scores than the nonconstipation group (P < 0.05, whereas HD patients had better SF-12 Physical Component

  9. Clinical course of nonalcoholic fatty liver disease: an assessment of severity, progression, and outcomes

    Directory of Open Access Journals (Sweden)

    Simeone JC

    2017-12-01

    Full Text Available Jason C Simeone,1 Jay P Bae,2 Byron J Hoogwerf,3 Qian Li,1 Axel Haupt,3 Ayad K Ali,4 Marilyn K Boardman,3 Beth L Nordstrom1 1Real-world Evidence, Evidera, Waltham, MA, USA; 2Global Patient Outcomes and Real World Evidence, Eli Lilly and Company, Indianapolis, IN, USA; 3Lily Diabetes, Eli Lilly and Company, Indianapolis, IN, USA; 4Global Patient Safety, Eli Lilly and Company, Indianapolis, IN, USA Purpose: To identify the characteristics and initial disease severity of patients with nonalcoholic fatty liver disease (NAFLD and assess incidence and risk factors for disease progression in a retrospective study.Methods: Patients ≥18 years of age without alcoholism or other liver diseases (eg, hepatitis B/C were selected from Geisinger Health System electronic medical record data from 2004 to 2015. Initial disease stage was stratified into uncomplicated NAFLD, advanced fibrosis, cirrhosis, hepatocellular carcinoma (HCC, and liver transplant using clinical biomarkers, diagnosis, and procedure codes. Disease progression was defined as stage progression or death and analyzed via Kaplan–Meier plots and multistate models.Results: In the NAFLD cohort (N=18,754, 61.5% were women, 39.0% had type 2 diabetes mellitus (T2DM, and the mean body mass index was 38.2±10.2 kg/m2. At index, 69.9% had uncomplicated NAFLD, 11.7% had advanced fibrosis, and 17.8% had cirrhosis. Of 18,718 patients assessed for progression, 17.3% progressed (11.0% had stage progression, 6.3% died without evidence of stage progression during follow-up (median=842 days. Among subgroups, 12.3% of those without diabetes mellitus progressed vs 24.7% of those with T2DM. One-year mortality increased from 0.5% in uncomplicated NAFLD to 22.7% in HCC. After liver transplant, mortality decreased to 5.6% per year.Conclusions: In 2.3 years of follow-up, approximately 17% of patients progressed or died without evidence of stage progression. T2DM was associated with approximately twice the risk of

  10. Enhancement of radiotherapy efficacy by miR-200c-loaded gelatinase-stimuli PEG-Pep-PCL nanoparticles in gastric cancer cells

    Directory of Open Access Journals (Sweden)

    Cui FB

    2014-05-01

    Full Text Available Fang-bo Cui,1,* Qin Liu,1,* Ru-Tian Li,1 Jie Shen,1 Pu-yuan Wu,1 Li-Xia Yu,1 Wen-jing Hu,1 Feng-lei Wu,2 Chun-Ping Jiang,1 Guo-feng Yue,2 Xiao-Ping Qian,1 Xi-Qun Jiang,3 Bao-Rui Liu11The Comprehensive Cancer Center of Drum-Tower Hospital, Medical School of Nanjing University and Clinical Cancer Institute of Nanjing University, Nanjing, 2Nanjing Medical University, 3Laboratory of Mesoscopic Chemistry and Department of Polymer Science and Engineering, College of Chemistry and Chemical Engineering, Nanjing University, Nanjing, People's Republic of China*These authors contributed equally to this workAbstract: Radiotherapy is the main locoregional control modality for many types of unresectable tumors, including gastric cancer. However, many patients fail radiotherapy due to intrinsic radioresistance of cancer cells, which has been found to be strongly associated with cancer stem cell (CSC-like properties. In this study, we developed a nanoparticle formulation to deliver miR-200c, which is reported to inhibit CSC-like properties, and then evaluated its potential activity as a radiosensitizer. miR-200c nanoparticles significantly augmented radiosensitivity in three gastric cancer cell lines (sensitization enhancement ratio 1.13–1.25, but only slightly in GES-1 cells (1.06. In addition to radioenhancement, miR-200c nanoparticles reduced the expression of CD44, a putative CSC marker, and the percentage of CD44+ BGC823 cells. Meanwhile, other CSC-like properties, including invasiveness and resistance to apoptosis, could be suppressed by miR-200c nanoparticles. CSC-associated radioresistance mechanisms, involving reactive oxygen species levels and DNA repair capacity, were also attenuated. We have demonstrated that miR-200c nanoparticles are an effective radiosensitizer in gastric cancer cells and induce little radiosensitization in normal cells, which suggests that they are as a promising candidate for further preclinical and clinical evaluation

  11. Concentration- and time-dependent response of human gingival fibroblasts to fibroblast growth factor 2 immobilized on titanium dental implants

    Directory of Open Access Journals (Sweden)

    Ma Q

    2012-04-01

    Full Text Available Qianli Ma1*, Wei Wang1*, Paul K Chu2, Shenglin Mei1,2, Kun Ji3, Lei Jin4, Yumei Zhang11Department of Prosthetic Dentistry, School of Stomatology, Fourth Military Medical University, Xi'an, People's Republic of China; 2Department of Physics and Materials Science, City University of Hong Kong, Kowloon, Hong Kong, People's Republic of China; 3Department of Pediatric Dentistry, School of Stomatology, Fourth Military Medical University, Xi'an, People's Republic of China; 4Stomatology Department, Jinling Hospital, School of Medicine, Southern Medical University, Nanjing, People's Republic of China*These authors contributed equally to this workBackground: Titanium (Ti implants are widely used clinically, but peri-implantitis remains one of the most common and serious complications. Healthy integration between gingival tissue and the implant surface is critical to long-term success in dental implant therapy. The objective of this study was to investigate how different concentrations of immobilized fibroblast growth factor 2 (FGF2 on the titania nanotubular surface influence the response of human gingival fibroblasts (HGFs.Methods: Pure Ti metal was anodized at 20 V to form a vertically organized titanium dioxide nanotube array on which three concentrations of FGF2 (250 ng/mL, 500 ng/mL, or 1000 ng/mL were immobilized by repeated lyophilization. Surface topography was observed and FGF2 elution was detected using enzyme-linked immunosorbent assay. The bioactivity changes of dissolvable immobilized FGF2 were measured by methyl-thiazolyl-tetrazolium assay. Behavior of HGFs was evaluated using adhesion and methyl-thiazolyl-tetrazolium bromide assays.Results: The FGF2 remained for several days on the modified surface on which HGFs were cultured. Over 90% of the dissolvable immobilized FGF2 had been eluted by Day 9, whereas the FGF2 activity was found to diminish gradually from Day 1 to Day 9. The titania nanotubular surface with an optimal preparing

  12. A phase I pharmacokinetic study of ursolic acid nanoliposomes in healthy volunteers and patients with advanced solid tumors

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    Ying G

    2013-01-01

    Full Text Available Zhongling Zhu,1,4 Zhengzi Qian,2,4 Zhao Yan,1,4 Cuicui Zhao,2,4 Huaqing Wang,2,4 Guoguang Ying3,41Department of Clinical Pharmacology, 2Department of Lymphoma, 3Laboratory of Tumor Cell Biology, Tianjin Medical University Cancer Institute and Hospital, Tianjin, People's Republic of China; 4Key Laboratory of Cancer Prevention and Therapy, Tianjin, People’s Republic of ChinaBackground: Ursolic acid is a promising anticancer agent. The current study aims to evaluate the single- and multiple-dose pharmacokinetics (PK as well as the safety of ursolic acid nanoliposomes (UANL in healthy volunteers and in patients with advanced solid tumors.Methods: Twenty-four healthy volunteers in the single-dose PK study were divided into three different groups, which received 37, 74, and 98 mg/m2 of UANL. Eight patients in the multiple-dose PK study were administered with 74 mg/m2 of UANL daily for 14 days. The UA plasma concentrations were determined using ultra-performance liquid chromatograph-tandem mass spectrometry.Results: The plasma concentration profiles of all subjects were characterized by a biexponential decline after infusion. The mean peak plasma concentration (Cmax increased linearly as a function of the dose (r = 0.999. The mean area under the plasma concentration-time curve (AUC from 0 to 16 hours also increased proportionally with dose escalation (r = 0.998. However, the clearance was constant over the specific dose interval. In the multiple-dose PK study, the trough and average concentrations remained low. The mean AUC, half-life, Cmax, time to Cmax, and the volume of distribution on the first day were similar to those on the last day. All subjects tolerated the treatments well. Most UANL-associated adverse events varied from mild to moderate.Conclusions: UANL exhibits relatively linear PK behavior with dose levels from 37 mg/m2 to 98 mg/m2. No drug accumulation was observed with repeated doses of UANL. The intravenous infusion of UANL was well

  13. Sulfonate-modified phenylboronic acid-rich nanoparticles as a novel mucoadhesive drug delivery system for vaginal administration of protein therapeutics: improved stability, mucin-dependent release and effective intravaginal placement

    Directory of Open Access Journals (Sweden)

    Li CY

    2016-11-01

    Full Text Available ChunYan Li,1 ZhiGang Huang,2 ZheShuo Liu,1 LiQian Ci,3 ZhePeng Liu,3 Yu Liu,2 XueYing Yan,1 WeiYue Lu2 1School of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin, 2Department of Pharmaceutics, School of Pharmacy, Key Laboratory of Smart Drug Delivery, Ministry of Education, Fudan University, 3School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, People’s Republic of China Abstract: Effective interaction between mucoadhesive drug delivery systems and mucin is the basis of effective local placement of drugs to play its therapeutic role after mucosal administration including vaginal use, which especially requires prolonged drug presence for the treatment of gynecological infectious diseases. Our previous report on phenylboronic acid-rich nanoparticles (PBNPs demonstrated their strong interaction with mucin and mucin-sensitive release profiles of the model protein therapeutics interferon (IFN in vitro, but their poor stability and obvious tendency to aggregate over time severely limited future application. In this study, sulfonate-modified PBNPs (PBNP-S were designed as a stable mucoadhesive drug delivery system where the negative charges conferred by sulfonate groups prevented aggregation of nanoparticles and the phenylboronic acid groups ensured effective interaction with mucin over a wide pH range. Results suggested that PBNP-S were of spherical morphology with narrow size distribution (123.5 nm, polydispersity index 0.050, good stability over a wide pH range and 3-month storage and considerable in vitro mucoadhesion capability at vaginal pH as shown by mucin adsorption determination. IFN could be loaded to PBNP-S by physical adsorption with high encapsulation efficiency and released in a mucin-dependent manner in vitro. In vivo near-infrared fluorescent whole animal imaging and quantitative vaginal lavage followed by enzyme-linked immunosorbent assay (ELISA assay of

  14. lncRNA H19 predicts poor prognosis in patients with melanoma and regulates cell growth, invasion, migration and epithelial–mesenchymal transition in melanoma cells

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    Shi G

    2018-06-01

    Full Text Available Gaofeng Shi,1,2 Hu Li,2 Fengshan Gao,2 Qian Tan1 1Drum Tower Clinical Medical College of Nanjing Medical University, Nanjing, People’s Republic of China; 2Department of Plastic Surgery, the Affiliated Wuxi No 4 People’s Hospital of Jiangnan University, Wuxi, People’s Republic of China Introduction: Melanoma is a deadly malignancy and the poor prognosis of patients with advanced disease is relatively poor. Recent studies indicate that long non-coding RNAs are involved in the pathogenesis of malignant melanoma. This study aims to investigate the role of the long non-coding RNA H19 in melanoma and to explore the underlying molecular mechanisms. Materials and methods: The expression levels of H19 in clinical samples and melanoma cells were determined by quantitative real-time PCR. The cell growth and cell metastasis were assessed by Cell Counting Kit 8, cell invasion and wound healing assays. Cell apoptosis and cell cycle were determined by flow cytometry. Protein levels were determined by Western blotting assay. Results: H19 was highly expressed in melanoma tissues compared to normal adjacent skin tissues, and the tissue expression level of H19 from melanoma patients with metastasis was significantly higher than that from patients without distant metastasis. In addition, the high expression of H19 in melanoma tissues was associated with advanced tumor invasion and TNM stage, distal metastasis, lymph node metastasis and shorter overall survival in patients with melanoma. The in vitro functional assays showed that knockdown of H19 inhibited cell growth, invasion and migration and also induced cell apoptosis as well as G0/G1 arrest in melanoma cells. Further quantitative real-time PCR and Western blot experiments showed that knockdown of H19 differentially regulated the epithelial–mesenchymal transition (EMT-related gene expressions and reversed EMT in melanoma cell lines. Knockdown of H19 suppressed in vivo tumor growth and modulated the

  15. WE-AB-207A-01: BEST IN PHYSICS (IMAGING): High-Resolution Cone-Beam CT of the Extremities and Cancellous Bone Architecture with a CMOS Detector

    International Nuclear Information System (INIS)

    Cao, Q; Brehler, M; Sisniega, A; Marinetto, E; Stayman, J; Siewerdsen, J; Zbijewski, W; Zyazin, A; Peters, I; Yorkston, J

    2016-01-01

    -frequency imaging tasks through adoption of the CMOS detector and small FS x-ray source, motivating the use of these components in a new system for quantitative in-vivo imaging of trabecular bone. Financial Support: US NIH grant R01EB018896. Qian Cao is a Howard Hughes Medical Institute International Student Research Fellow. Disclosures: W Zbijewski, J Siewerdsen and A Sisniega receive research funding from Carestream Health.

  16. Upregulation of vascular endothelial growth factor receptor-1 contributes to sevoflurane preconditioning–mediated cardioprotection

    Directory of Open Access Journals (Sweden)

    Qian B

    2018-04-01

    Full Text Available Bin Qian,1 Yang Yang,2 Yusheng Yao,3 Yanling Liao,3 Ying Lin3 1Department of Anesthesiology, People’s Hospital Affiliated to Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian, China; 2Department of Anesthesiology, West China Hospital, Sichuan University, Chengdu, Sichuan, China; 3Department of Anesthesiology, The Shengli Clinical Medical College, Fujian Medical University, Fuzhou, Fujian, China Purpose: Sevoflurane preconditioning (SPC can provide myocardial protective effects similar to ischemic preconditioning. However, the exact mechanism of SPC remains unclear. Previous studies indicate that vascular endothelial growth factor receptor 1 (VEGFR-1 is involved in ischemic preconditioning-mediated cardioprotection. This study was designed to determine the significance of VEGFR-1 signaling in SPC-mediated cardioprotection.Materials and methods: Myocardial ischemia–reperfusion (I/R rat model was established using the Langendorff isolated heart perfusion apparatus. Additionally, after 15 min of baseline equilibration, the isolated hearts were pretreated with 2.5% sevoflurane, 2.5% sevoflurane+MF1 10 µmol/L, or 2.5% sevoflurane+placental growth factor 10 µmol/L, and then subjected to 30 min of global ischemia and 120 min of reperfusion. The changes in hemodynamic parameters, myocardial infarct size, and the levels of creatine kinase-MB, lactate dehydrogenase, cardiac troponin-I, tumor necrosis factor-α, and interleukin 6 in the myocardium were evaluated.Results: Compared to the I/R group, pretreatment with 2.5% sevoflurane significantly improved the cardiac function, limited myocardial infarct size, reduced cardiac enzyme release, upregulated VEGFR-1 expression, and decreased inflammation. In addition, the selective VEGFR-1 agonist, placental growth factor, did not enhance the cardioprotection and anti-inflammation effects of sevoflurane, while the specific VEGFR-1 inhibitor, MF1, completely reversed these effects

  17. Induction of systemic and mucosal immunity against methicillin-resistant Staphylococcus aureus infection by a novel nanoemulsion adjuvant vaccine

    Directory of Open Access Journals (Sweden)

    Sun HW

    2015-12-01

    Full Text Available HongWu Sun,1,* Chao Wei,1,* BaoShuai Liu,1 HaiMing Jing,1 Qiang Feng,2 YaNan Tong,1 Yun Yang,1 LiuYang Yang,1 QianFei Zuo,1 Yi Zhang,1 QuanMing Zou,1 Hao Zeng1 1National Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy, College of Pharmacy, Third Military Medical University of Chinese PLA, 2Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing, People’s Republic of China *These authors contributed equally to this work Abstract: The Gram-positive bacterial pathogen methicillin-resistant Staphylococcus aureus (MRSA can cause infections in the bloodstream, endocardial tissue, respiratory tract, culture-confirmed skin, or soft tissue. There are currently no effective vaccines, and none are expected to become available in the near future. An effective vaccine capable of eliciting both systemic and mucosal immune responses is also urgently needed. Here, we reported a novel oil-in-water nanoemulsion adjuvant vaccine containing an MRSA recombination protein antigen, Cremophor EL-35® as a surfactant, and propylene glycol as a co-surfactant. This nanoemulsion vaccine, whose average diameter was 31.34±0.49 nm, demonstrated good protein structure integrity, protein specificity, and good stability at room temperature for 1 year. The intramuscular systemic and nasal mucosal immune responses demonstrated that this nanoemulsion vaccine could improve the specific immune responses of immunoglobulin (IgG and related subclasses, such as IgG1, IgG2a, and IgG2b, as well as IgA, in the serum after Balb/c mice intramuscular immunization and C57 mice nasal immunization. Furthermore, this nanoemulsion vaccine also markedly enhanced the interferon-γ and interleukin-17A cytokine cell immune response, improved the survival ratio, and reduced bacterial colonization. Taken together, our results show that this novel nanoemulsion vaccine has great potential and is a

  18. Reduced prefrontal activation during verbal fluency task in chronic insomnia disorder: a multichannel near-infrared spectroscopy study

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    Sun JJ

    2017-06-01

    Full Text Available Jing-Jing Sun,1,2 Xiao-Min Liu,2 Chen-Yu Shen,2 Xiao-Qian Zhang,1,2 Gao-Xiang Sun,2 Kun Feng,2 Bo Xu,2 Xia-Jin Ren,1,2 Xiang-Yun Ma,1,2 Po-Zi Liu2 1Medical Center, Tsinghua University, Beijing, China, 2Department of Psychiatry, YuQuan Hospital, Tsinghua University, Beijing, China Purpose: Daytime complaints such as memory and attention deficits and failure to accomplish daily tasks are common in insomnia patients. However, objective psychological tests to detect cognitive impairment are equivocal. Neural function associated with cognitive performance may explain the discrepancy. The aim of this study was to investigate the hemodynamic response patterns of patients with chronic insomnia disorder (CID using the noninvasive and low-cost functional neuroimaging technique of multichannel near-infrared spectroscopy (NIRS in order to identify changes of neural function associated with cognitive performance.Patients and methods: Twenty-four CID patients and twenty-five healthy controls matched for age, right-hand dominance, educational level, and gender were examined during verbal fluency tasks (VFT using NIRS. A covariance analysis was conducted to analyze differences of oxygenated hemoglobin (oxy-Hb changes in prefrontal cortex (PFC between the two groups and reduce the influence of the severity of depression. Pearson correlation coeffcients were calculated to examine the relationship between the oxy-Hb changes, with the severity of insomnia and depressive symptoms assessed by the Pittsburgh Sleep Quality Index (PSQI and the Hamilton Rating Scale for Depression (HAMD.Results: The number of words generated during the VFT in CID groups showed no statistical differences with healthy controls. CID patients showed hypoactivation in the PFC during the cognitive task. In addition, we found that the function of left orbitofrontal cortex (OFC during the VFT was significantly negatively correlated with the PSQI scores and the function of right dorsolateral PFC

  19. Leptin signaling molecular actions and drug target in hepatocellular carcinoma

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    Jiang N

    2014-11-01

    Full Text Available Nan Jiang,1,* Rongtong Sun,2,* Qing Sun3 1Shandong University School of Medicine, Jinan, Shandong Province, People’s Republic of China; 2Weihai Municipal Hospital, Weihai, Shandong Province, People’s Republic of China; 3Department of Pathology, QianFoShan Hospital Affiliated to Shandong University, Jinan, Shandong Province, People’s Republic of China *These authors contributed equally to this work Abstract: Previous reports indicate that over 13 different tumors, including hepatocellular carcinoma (HCC, are related to obesity. Obesity-associated inflammatory, metabolic, and endocrine mediators, as well as the functioning of the gut microbiota, are suspected to contribute to tumorigenesis. In obese people, proinflammatory cytokines/chemokines including tumor necrosis factor-alpha, interleukin (IL-1 and IL-6, insulin and insulin-like growth factors, adipokines, plasminogen activator inhibitor-1, adiponectin, and leptin are found to play crucial roles in the initiation and development of cancer. The cytokines induced by leptin in adipose tissue or tumor cells have been intensely studied. Leptin-induced signaling pathways are critical for biological functions such as adiposity, energy balance, endocrine function, immune reaction, and angiogenesis as well as oncogenesis. Leptin is an activator of cell proliferation and anti-apoptosis in several cell types, and an inducer of cancer stem cells; its critical roles in tumorigenesis are based on its oncogenic, mitogenic, proinflammatory, and pro-angiogenic actions. This review provides an update of the pathological effects of leptin signaling with special emphasis on potential molecular mechanisms and therapeutic targeting, which could potentially be used in future clinical settings. In addition, leptin-induced angiogenic ability and molecular mechanisms in HCC are discussed. The stringent binding affinity of leptin and its receptor Ob-R, as well as the highly upregulated expression of both

  20. The preosteoblast response of electrospinning PLGA/PCL nanofibers: effects of biomimetic architecture and collagen I

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    Qian YZ

    2016-08-01

    Full Text Available Yunzhu Qian,1,2 Hanbang Chen,1 Yang Xu,1 Jianxin Yang,2 Xuefeng Zhou,3 Feimin Zhang,1 Ning Gu3 1Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, 2Center of Stomatology, The Second Affiliated Hospital of Soochow University, Suzhou, 3School of Biological Science and Medical Engineering, Southeast University, Nanjing, People’s Republic of China Abstract: Constructing biomimetic structure and incorporating bioactive molecules is an effective strategy to achieve a more favorable cell response. To explore the effect of electrospinning (ES nanofibrous architecture and collagen I (COL I-incorporated modification on tuning osteoblast response, a resorbable membrane composed of poly(lactic-co-glycolic acid/poly(caprolactone (PLGA/PCL; 7:3 w/w was developed via ES. COL I was blended into PLGA/PCL solution to prepare composite ES membrane. Notably, relatively better cell response was delivered by the bioactive ES-based membrane which was fabricated by modification of 3,4-dihydroxyphenylalanine and COL I. After investigation by field emission scanning electron microscopy, Fourier transform infrared spectroscopy, contact angle measurement, and mechanical test, polyporous three-dimensional nanofibrous structure with low tensile force and the successful integration of COL I was obtained by the ES method. Compared with traditional PLGA/PCL membrane, the surface hydrophilicity of collagen-incorporated membranes was largely enhanced. The behavior of mouse preosteoblast MC3T3-E1 cell infiltration and proliferation on membranes was studied at 24 and 48 hours. The negative control was fabricated by solvent casting. Evaluation of cell adhesion and morphology demonstrated that all the ES membranes were more favorable for promoting the cell adhesion and spreading than the casting membrane. Cell Counting Kit-8 assays revealed that biomimetic architecture, surface topography, and bioactive properties of membranes were favorable for cell

  1. Power, identity and antiquarian approaches in modern Chinese art’

    Directory of Open Access Journals (Sweden)

    Chia-Ling Yang

    2014-06-01

    Full Text Available The pursuit of antiquity was important for scholarly artists in constructing their knowledge of history and cultural identity in late Imperial China. Following various publications by Bi Yuan 畢沅 (1730-1797, Wu Yi 武億 (1745-1799 and Qian Daxin 錢大昕 (1728-1804 in the 18th century, the study and collecting of rubbings of Northern Wei stone inscriptions and steles was popular. Such spread of interest in jinshi, inscriptions on metal and stone, also formed a base for studying seal carving, epigraphy and archaic painting. While traditional antiquarians would cherish inscriptions which enabled them to correct mistakes in the transmitted historical texts and the Classics, however, much of the antiquarian activity was adapted to mere literary exercise or connoisseurship, for instance, to supplying materials which could provide models for seal-carving and calligraphy. Examples could be seen in the calligraphy works and seal carvings of the Xiling bajia 西泠八家 (Eight Masters of Xiling, i.e. Hangzhou, also known as Zhe School of Calligraphy and Carving. Their keen interest in seeking inspiration from steles for their artistic presentations has been recorded in their writing and painting. In addition, the way the scholar-collector of the 19th and early 20th centuries mounted the rubbings, seals, inscriptions, paintings, letters and textual evidence studies into one album shows a changing ideology: rubbings were not only for scholarly study in classical learning, but were regarded as part of the art form and were appreciated on various social occasions. The antiquarian movement ultimately served as a tool for re-writing art historiography in modern China. This paper aims to address the phenomenon and formation of the jinshi painting that dominated in late Imperial and early modern China. Through case studies of three important jinshi societies in Shanghai, I will investigate in what way literary taste from the southern region gradually

  2. Preparation and characterization of monomethoxy poly(ethylene glycol-poly(ε-caprolactone micelles for the solubilization and in vivo delivery of luteolin

    Directory of Open Access Journals (Sweden)

    Qiu JF

    2013-08-01

    Full Text Available Jin-Feng Qiu,1 Xiang Gao,1,2 Bi-Lan Wang,1 Xia-Wei Wei,1 Ma-Ling Gou,1 Ke Men,1 Xing-Yu Liu,1 Gang Guo,1 Zhi-Yong Qian,1 Mei-Juan Huang1 1Department of Thoracic Oncology, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital and Medical School, Sichuan University, Chengdu, People’s Republic of China; 2Medical School and Department of Pathophysiology, College of Preclinical and Forensic Medical Sciences, Sichuan University, Chengdu, People’s Republic of China Abstract: Luteolin (Lu is one of the flavonoids with anticancer activity, but its poor water solubility limits its use clinically. In this work, we used monomethoxy poly(ethylene glycol-poly(ε-caprolactone (MPEG-PCL micelles to encapsulate Lu by a self-assembly method, creating a water-soluble Lu/MPEG-PCL micelle. These micelles had a mean particle size of 38.6 ± 0.6 nm (polydispersity index = 0.16 ± 0.02, encapsulation efficiency of 98.32% ± 1.12%, and drug loading of 3.93% ± 0.25%. Lu/MPEG-PCL micelles could slowly release Lu in vitro. Encapsulation of Lu in MPEG-PCL micelles improved the half-life (t½; 152.25 ± 49.92 versus [vs] 7.16 ± 1.23 minutes, P = 0.007, area under the curve (0–t (2914.05 ± 445.17 vs 502.65 ± 140.12 mg/L/minute, P = 0.001, area under the curve (0–∞ (2989.03 ± 433.22 vs 503.81 ± 141.41 mg/L/minute, P = 0.001, and peak concentration (92.70 ± 11.61 vs 38.98 ± 7.73 mg/L, P = 0.003 of Lu when the drug was intravenously administered at a dose of 30 mg/kg in rats. Also, Lu/MPEG-PCL micelles maintained the cytotoxicity of Lu on 4T1 breast cancer cells (IC50 = 6.4 ± 2.30 µg/mL and C-26 colon carcinoma cells (IC50 = 12.62 ± 2.17 µg/mL in vitro. These data suggested that encapsulation of Lu into MPEG-PCL micelles created an aqueous formulation of Lu with potential anticancer effect. Keywords: luteolin, micelle, MPEG-PCL, cancer therapy

  3. Multifunctional dendrimer-based nanoparticles for in vivo MR/CT dual-modal molecular imaging of breast cancer

    Directory of Open Access Journals (Sweden)

    Li K

    2013-07-01

    Full Text Available Kangan Li,1,4,5,* Shihui Wen,2,* Andrew C Larson,4,5 Mingwu Shen,2 Zhuoli Zhang,4,5 Qian Chen,3 Xiangyang Shi,2,3 Guixiang Zhang1 1Department of Radiology, Shanghai First People’s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, People’s Republic of China; 2College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, People’s Republic of China; 3State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Donghua University, Shanghai, People’s Republic of China; 4Departments of Radiology and Biomedical Engineering, Northwestern University, Chicago, IL, USA; 5Robert H Lurie Comprehensive Cancer Center, Chicago, IL, USA *These authors contributed equally to this work Abstract: Development of dual-mode or multi-mode imaging contrast agents is important for accurate and self-confirmatory diagnosis of cancer. We report a new multifunctional, dendrimer-based gold nanoparticle (AuNP as a dual-modality contrast agent for magnetic resonance (MR/computed tomography (CT imaging of breast cancer cells in vitro and in vivo. In this study, amine-terminated generation 5 poly(amidoamine dendrimers modified with gadolinium chelate (DOTA-NHS and polyethylene glycol monomethyl ether were used as templates to synthesize AuNPs, followed by Gd(III chelation and acetylation of the remaining dendrimer terminal amine groups; multifunctional dendrimer-entrapped AuNPs (Gd-Au DENPs were formed. The formed Gd-Au DENPs were used for both in vitro and in vivo MR/CT imaging of human MCF-7 cancer cells. Both MR and CT images demonstrate that MCF-7 cells and the xenograft tumor model can be effectively imaged. The Gd-Au DENPs uptake, mainly in the cell cytoplasm, was confirmed by transmission electron microscopy. The cell cytotoxicity assay, cell morphology observation, and flow cytometry show that the developed Gd-Au DENPs have good biocompatibility in the given concentration range. Our results

  4. EDITORIAL: Focus on Quantum Information and Many-Body Theory

    Science.gov (United States)

    Eisert, Jens; Plenio, Martin B.

    2010-02-01

    Quantum many-body models describing natural systems or materials and physical systems assembled piece by piece in the laboratory for the purpose of realizing quantum information processing share an important feature: intricate correlations that originate from the coherent interaction between a large number of constituents. In recent years it has become manifest that the cross-fertilization between research devoted to quantum information science and to quantum many-body physics leads to new ideas, methods, tools, and insights in both fields. Issues of criticality, quantum phase transitions, quantum order and magnetism that play a role in one field find relations to the classical simulation of quantum systems, to error correction and fault tolerance thresholds, to channel capacities and to topological quantum computation, to name but a few. The structural similarities of typical problems in both fields and the potential for pooling of ideas then become manifest. Notably, methods and ideas from quantum information have provided fresh approaches to long-standing problems in strongly correlated systems in the condensed matter context, including both numerical methods and conceptual insights. Focus on quantum information and many-body theory Contents TENSOR NETWORKS Homogeneous multiscale entanglement renormalization ansatz tensor networks for quantum critical systems M Rizzi, S Montangero, P Silvi, V Giovannetti and Rosario Fazio Concatenated tensor network states R Hübener, V Nebendahl and W Dür Entanglement renormalization in free bosonic systems: real-space versus momentum-space renormalization group transforms G Evenbly and G Vidal Finite-size geometric entanglement from tensor network algorithms Qian-Qian Shi, Román Orús, John Ove Fjærestad and Huan-Qiang Zhou Characterizing symmetries in a projected entangled pair state D Pérez-García, M Sanz, C E González-Guillén, M M Wolf and J I Cirac Matrix product operator representations B Pirvu, V Murg, J I Cirac

  5. PREFACE International Symposium on Spintronic Devices and Commercialization 2010

    Science.gov (United States)

    Du, You-wei; Judy, Jack; Qian, Zhenghong; Wang, Jianping

    2011-01-01

    very successful indeed. We are also grateful to the Advisory Committee, the Conference Chairs, the Excutive Chairs, the Academic Committee, the Organization Committee and the Secretariat for their fruitful work. We would especially like to thank all the organizers listed below for their support in all aspects of the conference. We would like to express our thanks to all the authors for their time and genuine efforts, and to the reviewers for their fruitful comments during the preparation of this volume. ISSDC'2010 ORGANIZATION Advisory Committee Chialing Chien, USAJunhao Chu, ChinaBernard Dieny, FranceKoichiro Inomata, Japan Liangmo Mei, ChinaJohn Sivertsen, USAMingjing Tu, ChinaDingsheng Wang, China Zhanguo Wang, ChinaQikun Xue, ChinaWenshan Zhan, China Conference Chairs Jack Judy, USAYouwei Du, China Executive Chairs Zhenghong Qian, USAJianping Wang, USA Organization Committee ChairJiyan Luo, China Vice ChairsGuilin Duan, ChinaLingling Sun, ChinaBaogen Shen, China MembersTiecheng Lu, ChinaDa Ma, ChinaYe Tian, China Jinsong Xu, ChinaQiuling XuChangmao Yang, China Guanghua Yu, ChinaYi Yan, China Academic Committee ChairsZhenghong Qian, USAYongbing Xu, UK Vice ChairsSeongtae Bae, SingaporeYong Jiang, ChinaDexin Wang, USA Huaiwu Zhang, ChinaJianhua Zhao, China MembersJianwang Cai, ChinaXiangdong Chen, ChinaHaifeng Ding, China Chunhong Hou, USAGunther Baubock, USABin Hu, USA Jungchun Huang, TaiwanDexuan Huo, ChinaYoon H Jeong, Korea Chihuang Lai, TaiwanRunwei Li, ChinaWei Liu, China Jing Shi, USAYasushi Takemura, JapanMark Tondra, USA Shan X Wang, USADi Wu, ChinaDesheng Xue, China Minglang Yan, USAShishen Yan, ChinaXiaofei Yang, China Chun Yeol You, KoreaWei Zhao, ChinaShiming Zhou, China Jianguo Zhu, China Secretariat Secretary-generalChangmao Yang, China Vice Secretary-generalJunli Wang, ChinaJinsong Xu, ChinaYe Tian, China MembersRu Bai, ChinaHongliang Zhan, China ISSDC' 2010 Organizers Department of Science and Technology, CSIC, China SpinIC Inc., China Hangzhou

  6. Cycle ergometer and inspiratory muscle training offer modest benefit compared with cycle ergometer alone: a comprehensive assessment in stable COPD patients

    Directory of Open Access Journals (Sweden)

    Wang K

    2017-09-01

    Full Text Available Kai Wang,1,* Guang-qiao Zeng,2,* Rui Li,1,* Yu-wen Luo,1 Mei Wang,1 Yu-he Hu,1 Wen-hui Xu,1 Lu-qian Zhou,2 Rong-chang Chen,2 Xin Chen1 1Department of Respiratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, China; 2Department of Respiratory Medicine, The State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Disease, First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China *These authors contributed equally to this work Background: Cycle ergometer training (CET has been shown to improve exercise performance of the quadriceps muscles in patients with COPD, and inspiratory muscle training (IMT may improve the pressure-generating capacity of the inspiratory muscles. However, the effects of combined CET and IMT remain unclear and there is a lack of comprehensive assessment.Materials and methods: Eighty-one patients with COPD were randomly allocated to three groups: 28 received 8 weeks of CET + IMT (combined training group, 27 received 8 weeks of CET alone (CET group, and 26 only received 8 weeks of free walking (control group. Comprehensive assessment including respiratory muscle strength, exercise capacity, pulmonary function, dyspnea, quality of life, emotional status, nutritional status, and body mass index, airflow obstruction, and exercise capacity index were measured before and after the pulmonary rehabilitation program.Results: Respiratory muscle strength, exercise capacity, inspiratory capacity, dyspnea, quality of life, depression and anxiety, and nutritional status were all improved in the combined training and CET groups when compared with that in the control group (P<0.05 after pulmonary rehabilitation program. Inspiratory muscle strength increased significantly in the combined training group when compared with that in the CET group (ΔPImax [maximal inspiratory pressure] 5.20±0.89 cmH2O vs 1.32±0.91 cmH2O

  7. Collaborative International Evaluated Library Organisation Pilot Project - CIELO meeting, OECD Conference Centre, 9-11 May 2016

    International Nuclear Information System (INIS)

    Herman, Michal Wladyslaw; Cabellos De Francisco, Oscar; Trkov, Andrej; Bauge, Eric; Conlin, Jeremy Lloyd; Ignatyuk, Anatoly V.; Mcnabb, Dennis P.; Palmiotti, Giuseppe; Grudzevich, Oleg T.; Mattoon, Caleb; Brown, David; Chadwick, Mark; Roubtsov, Danila; Iwamoto, Osamu; Kahler, Albert C.; Diez De La Obra, Carlos Javier; Qian, Jing; Wu, Haicheng; Ruan, Xichao; Sobes, Vladimir; Rearden, Bradley T.; Yokoyama, Kenji; Schillebeeckx, Peter; Kodeli, Ivan-Alexander; Plompen, Arjan; White, Morgan C.; Leal, Luiz Carlos; Fiorito, Luca; Danon, Yaron; Romain, Pascal; Dunn, Michael; Zerovnik, Gasper; Morillon, Benjamin; Jacqmin, Robert

    2016-05-01

    The aim of WPEC subgroup 39 'Methods and approaches to provide feedback from nuclear and covariance data adjustment for improvement of nuclear data files' is to provide criteria and practical approaches to use effectively the results of sensitivity analyses and cross section adjustments for feedback to evaluators and differential measurement experimentalists in order to improve the knowledge of neutron cross sections, uncertainties, and correlations to be used in a wide range of applications. WPEC subgroup 40-CIELO (Collaborative International Evaluated Library Organization) provides a new working paradigm to facilitate evaluated nuclear reaction data advances. It brings together experts from across the international nuclear reaction data community to identify and document discrepancies among existing evaluated data libraries, measured data, and model calculation interpretations, and aims to make progress in reconciling these discrepancies to create more accurate ENDF-formatted files. SG40-CIELO focusses on 6 important isotopes: "1H, "1"6O, "5"6Fe, "2"3"5","2"3"8U, "2"3"9Pu. This document is the proceedings of the 2016 SG40-CIELO meeting, followed by a joint SG39/SG40 session, held at the OECD Headquarters Conference Center, Paris, France, on 9-11 May 2016. It comprises all the available presentations (slides) given by the participants: A - SG40-CIELO meeting: - SG40-1: Status of Cross Section Progress for "2"3"5","8U, "2"3"9Pu, "5"6Fe, "1"6O (Mark CHADWICK); - SG40-2: Summary of IRMM (Arjan PLOMPEN); - SG40-2.1: Giorginis "1"6O(n,alpha) insights (Arjan PLOMPEN); - SG40-3: New Oxygen "1"6O Hale evaluation (Mark CHADWICK); - SG40-4: "1"6O and "5"6Fe Iron resonance region evaluations (Luiz LEAL); - SG40-5: Iron evaluation work at BNL, ORNL, and IAEA (Mike HERMAN); - SG40-6: Minor Fe isotopes (David BROWN); - SG40-7: Iron evaluation work at CIAE (Jing QIAN); - SG40-8: IAEA CIELO data testing relevant to "5"6Fe (Andre TRKOV); - SG40-9: CIELO data testing (Skip KAHLER

  8. Metamorphic and tectonic evolution of the Greater Himalayan Crystalline Complex in Nyalam region, south Tibet

    Science.gov (United States)

    Wang, Jia-Min; Zhang, Jin-Jiang; Rubatto, Daniela

    2016-04-01

    timing along the South Tibetan detachment (27-16 Ma) but precedes that along the MCT (16-10 Ma). Comparison between the obtained P-T-t data and model predictions implies that a lateral crustal flow process dominated the exhumation of the high-grade upper GHC migmitites during 25-16 Ma, whereas a critical taper thrusting process dominated the exhumation of the MCT zone nonmigmatites and cooled migmatites in the lower GHC at 16-10 Ma. In other words, at different temporal and spatial scale, both propagating thrusting along large tectonic boundaries and a low-viscosity melting crust could contribute to the exhumation of high-grade metamorphic rocks in Himalaya-like large hot collisional orogens. KEY WORDS: Greater Himalayan Crystalline Complex; P-T path; U-Pb geochronology; channel flow; tectonic discontinuity References: Wang, J.M., Rubatto, D., Zhang, J.J., 2015a. Timing of partial melting and cooling across the Greater Himalayan Crystalline Complex (Nyalam, central Himalaya): in-sequence thrusting and its implications. Journal of Petrology, 56, 1677-1702. Wang, J.M., Zhang, J.J., Wei, C.J., Rai, S.M., Wang, M., Qian, J.H., 2015b. Characterizing the metamorphic discontinuity across the Main Central Thrust Zone of eastern-central Nepal. Journal of Asian Earth Sciences 101, 83-100. Wang, J.M., Zhang, J.J., Wang, X.X., 2013. Structural kinematics, metamorphic P-T profiles and zircon geochronology across the Greater Himalayan Crystalline Complex in south-central Tibet: implication for a revised channel flow. Journal of Metamorphic Geology 31, 607-628.

  9. Comparison of planning target volumes based on three-dimensional and four-dimensional CT imaging of thoracic esophageal cancer

    Directory of Open Access Journals (Sweden)

    Wang W

    2016-08-01

    Full Text Available Wei Wang, Jianbin Li, Yingjie Zhang, Qian Shao, Min Xu, Tingyong Fan, Jinzhi Wang Department of Radiation Oncology, Shandong Cancer Hospital Affiliated to Shandong University, Shandong Academy of Medical Sciences, Shandong, People’s Republic of China Background and purpose: To investigate the definition of planning target volumes (PTVs based on four-dimensional computed tomography (4DCT compared with conventional PTV definition and PTV definition using asymmetrical margins for thoracic primary esophageal cancer. Materials and methods: Forty-three patients with esophageal cancer underwent 3DCT and 4DCT simulation scans during free breathing. The motions of primary tumors located in the proximal (group A, middle (group B, and distal (group C thoracic esophagus were obtained from the 4DCT scans. PTV3D was defined on 3DCT using the tumor motion measured based on 4DCT, PTV conventional (PTVconv was defined on 3DCT by adding a 1.0 cm margin to the clinical target volume, and PTV4D was defined as the union of the target volumes contoured on the ten phases of the 4DCT images. The centroid positions, volumetric differences, and dice similarity coefficients were evaluated for all PTVs. Results: The median centroid shifts between PTV3D and PTV4D and between PTVconv and PTV4D in all three dimensions were <0.3 cm for the three groups. The median size ratios of PTV4D to PTV3D were 0.80, 0.88, and 0.71, and PTV4D to PTVconv were 0.67, 0.73, and 0.76 (χ2=–3.18, –2.98, and –3.06; P=0.001, 0.003, and 0.002 for groups A, B, and C, respectively. The dice similarity coefficients were 0.87, 0.90, and 0.81 between PTV4D and PTV3D and 0.80, 0.84, and 0.83 between PTV4D and PTVconv (χ2=–3.18, –2.98, and –3.06; P=0.001, 0.003, and 0.002 for groups A, B, and C, respectively. The difference between the degree of inclusion of PTV4D in PTV3D and that of PTV4D in PTVconv was <2% for all groups. Compared with PTVconv, the amount of irradiated normal tissue

  10. Electromagnetic Emissions During Rock-fracturing Experiments Inside Magnetic Field Free Space

    Science.gov (United States)

    Wang, H.; Zhou, J.; Zhu, T.; Jin, H.

    2012-12-01

    Corporation in America. EME and AE signals were collected at sampling rate larger than 1 MHz, while strain signals at sampling interval of 0.1 second. Abnormal strong EME signals were usually observed at the same moment with the main crack indicated by strain signals. There are too many AE signals but too little EME signals collected during the rock-cracking experiments. Even AE signals with amplitude larger than 60dB were hardly found accompanied by EME signals. Few data indicate that EME and AE signals occur simultaneously. Abnormal EME signals were always detected after rock specimens were cracked, indicated by the torn strain gauges. The lag of EME signals behind the cracking signals revealed by strain gauges may indicate that EME signals were caused by the fast movement of charged rock fragments. This study was sponsored by National Natural Science Foundation of China (NSFC, grant 41004029) and Institute of Geophysics, CEA (Project No.: DQJB10B14). We would like to thank Shuqing Qian, Zhiwei Guo and Zhengyi Liu to take part in the experiments.

  11. PREFACE: Preface

    Science.gov (United States)

    Audoin, Bertrand; Dehoux, Thomas; Guillet, Yannick

    2011-02-01

    and the gala dinner took place in a wine chateau in the medieval village. We hope this experience will remain in the attendees' memories as a pleasant and convivial time. We would like to express our thanks to the members of the organizing committee, the scientific committee, and all our generous sponsors, either institutions or companies, for their help in making this event possible. We would also like to thank the scientists involved for their confidence in our organization, and for their contributions. As unanimously decided by the attendees, the next symposium will be held in Japan, most likely in 2012. The papers published in this volume of the Journal of Physics: Conference Series provide a collection of state-of-the-art and recent advances in research and applications of laser ultrasonics as presented at this second Symposium. Bertrand Audoin - Conference ChairThomas Dehoux - Conference proceedings co-editorYannick Guillet - Conference proceedings co-editor Conference photograph Scientific committee Bertrand AudoinUniversité Bordeaux 1, France Alain BlouinNRC, Canada Christ GlorieuxKatholieke Universiteit Leuven, Belgium Vitalyi GusevUniversité du Maine, France Peter HessUniversity of Heidelberg, Germany Alexander A KarabutovMoscow State University, Russia Sridhar KrishnaswamyNorthwestern University, USA Humphrey MarisBrown University, USA Jean-Pierre MonchalinNRC, Canada Todd MurrayBoston University, USA Makoto OchiaiToshiba Corp, Japan Menglu QianTongji University, China Daniel RoyerEcole Supérieure de Physique et de Chimie, France Hervé TrétoutDassault Aviation, France Hubert VoillaumeEADS, France Oliver B WrightHokkaido University, Japan Shu-yi ZhangNanjing University, China Organizing committee Bertrand AudoinChair Sandrine GuitLocal administrator Christophe Aristégui Thomas Dehoux Evelyne Dewayse Yannick Guillet Armelle Guilloux

  12. Effects of a micro/nano rough strontium-loaded surface on osseointegration

    Directory of Open Access Journals (Sweden)

    Li Y

    2015-07-01

    Full Text Available Yongfeng Li,1,* Yaping Qi,2,* Qi Gao,3,* Qiang Niu,1 Mingming Shen,2 Qian Fu,1 Kaijin Hu,1 Liang Kong1 1State Key Laboratory of Military Stomatology, Department of Oral and Maxillofacial Surgery, School of Stomatology, the Fourth Military Medical University, Xi’an, 2Department of Oral and Maxillofacial Surgery, School of Stomatology, the Second Hospital of Hebei Medical University, Shijiazhuang, 3Department of Stomatology, PLA 458 Hospital, Guangzhou, People’s Republic of China *These authors contributed equally to this work Abstract: We developed a hierarchical hybrid micro/nanorough strontium-loaded Ti (MNT-Sr surface fabricated through hydrofluoric acid etching followed by magnetron sputtering and evaluated the effects of this surface on osseointegration. Samples with a smooth Ti (ST surface, micro Ti (MT surface treated with hydrofluoric acid etching, and strontium-loaded nano Ti (NT-Sr surface treated with SrTiO3 target deposited via magnetron sputtering technique were investigated in parallel for comparison. The results showed that MNT-Sr surfaces were prepared successfully and with high interface bonding strength. Moreover, slow Sr release could be detected when the MNT-Sr and NT-Sr samples were immersed in phosphate-buffered saline. In in vitro experiments, the MNT-Sr surface significantly improved the proliferation and differentiation of osteoblasts compared with the other three groups. Twelve weeks after the four different surface implants were inserted into the distal femurs of 40 rats, the bone–implant contact in the ST, MT, NT-Sr, and MNT-Sr groups were 39.70%±6.00%, 57.60%±7.79%, 46.10%±5.51%, and 70.38%±8.61%, respectively. In terms of the mineral apposition ratio, the MNT-Sr group increased by 129%, 58%, and 25% compared with the values of the ST, MT, and NT-Sr groups, respectively. Moreover, the maximal pullout force in the MNT-Sr group was 1.12-, 0.31-, and 0.69-fold higher than the values of the ST, MT, and NT

  13. SPAG9 is involved in hepatocarcinoma cell migration and invasion via modulation of ELK1 expression

    Directory of Open Access Journals (Sweden)

    Yan QY

    2016-03-01

    Full Text Available Qiuyue Yan,1,2 Guohua Lou,3 Ying Qian,1 Bo Qin,1 Xiuping Xu,1,2 Yanan Wang,1,2 Yanning Liu,3 Xuejun Dong1 1Shaoxing People’s Hospital, Shaoxing Hospital Zhejiang University, Shaoxing, Zhejiang, 2The Key Laboratory of Laboratory Medicine, Ministry of Education of China, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, 3State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, People’s Republic of China Background: Sperm-associated antigen 9 (SPAG9 is upregulated in several malignancies and its overexpression is positively correlated with cancer cell malignancies. However, the specific biological roles of SPAG9 in hepatocellular carcinoma (HCC are less understood. Methods: We analyzed SPAG9 and ETS-like gene 1, tyrosine kinase (ELK1 expression in 50 paired HCC specimens and adjacent noncancerous liver specimens using immunohistochemistry. SPAG9 small interfering RNA (siRNA was used to knockdown SPAG9 expression in HCCLM3 and HuH7 cell lines. We used plasmids to upregulate ELK1 expression and siRNA to downregulate ELK1 expression in HuH7 cells. Quantitative real-time polymerase chain reaction and Western blot were used to evaluate the expression of SPAG9 and ELK1 at the mRNA and protein level, respectively. Wound healing, matrigel migration, and invasion analyses were performed to determine the effect of SPAG9 and ELK1 on HCC metastasis. Results: SPAG9 and ELK1 were overexpressed in HCC tissue specimens and their expressions were higher in HCCLM3 and HuH7 cells compared to the low-metastatic HepG2 cells. Overexpression of SPAG9 was positively associated with tumor-node-metastasis staging (P=0.032, metastasis parameters (P=0.018 of HCC patients, and ELK1 expression (r=0.422, P<0.001 in HCC tissue specimens. In addition

  14. What influences climate and glacier change in southwestern China?

    Science.gov (United States)

    Yasunari, Teppei J.

    2011-12-01

    as a collective view. However, the precipitation patterns in southwestern China are probably modulated by climate feedbacks through many factors. Precipitation seasonality may also affect the climatic sensitivity of glacier mass balance (Fujita 2008). In addition to the authors' main focus above, other factors, also probably directly and indirectly, influence the climate and glacier mass balance changes. Those factors are: (a) The debris-covered effect which heats (if it is thin) or insulates (if it is thick) the ice below the debris; it probably causes no uniform response on glacier melting (Scherler et al 2011); (b) Interaction between glacial lakes and exposed ice parts on glaciers (e.g., Sakai et al 2009, Fujita et al 2009); (c) The atmospheric heating effect over the foothills of the Himalayas due to the Atmospheric Brown Cloud (ABC), including absorbing aerosols such as black carbon, dust and organic matters (Ramanathan et al 2007), the so called Elevated Heat Pump (EHP) effect suggested by Lau et al (2006, 2010); (d) The snow darkening effect over non debris-covered parts of glaciers as the absorbing aerosol depositions reduce snow albedo and accelerate snow melting by absorbing more solar energy at the snow surface (Warren and Wiscombe 1980, Flanner et al 2007, 2009, Yasunari et al 2010, Qian et al 2011); (e) Another kind of snow darkening effect over non debris-covered glaciers due to the growth of biological activities, with dark-colored materials on glaciers also reducing snow albedo (Takeuchi et al 2001); (f) Other factors on snow albedo reductions such as snow grain size, specific surface area and depth changes, melt-water effect on snow, and changes in solar illumination conditions (e.g., Wiscombe and Warren 1980, Flanner et al 2006, Yasunari et al 2011, Aoki et al 1999, 2011); and finally, (g) Feedbacks via interactions between the snow surface and atmosphere including all the factors above. What I'd like to emphasize is that the atmospheric warming

  15. PREFACE: Quark Matter 2006 Conference

    Science.gov (United States)

    Ma, Yu-Gang; Wang, En-Ke; Cai, Xu; Huang, Huan-Zhong; Wang, Xin-Nian; Zhu, Zhi-Yuan

    2007-07-01

    oral presentations. Professors T Hemmick, H Satz, D T Son and N Xu gave excellent pedagogical lectures in the pre-conference student symposium and their efforts were greatly appreciated. The Shanghai Association of Science and Technology (SAST) and the staff of the Shanghai science hall provided valuable assistance and services during the conference. The conference would not have run so smoothly without their professional dedication. We also thank Professor Wenqing Shen, Chairman of the SAST, for his many valuable suggestions to the conference organizers and for providing close cooperation with SAST staff. We thank members of the Local Organizing Committee for many useful suggestions and help. We would like to express our special appreciation for the tireless efforts by many local staff who worked very hard to make this conference a success. Dr Xiang-Zhou Cai, Mrs Wei Zhou and Mrs Yang Shen undertook many duties to coordinate and organize the local services. Dr Kun Wang took responsibility of the conference web page. Dr Wei Guo, Dr Wendong Tian, Mr Chunwang Ma and Mrs Wanyan Qian organized student volunteers for the conference. Without their help and dedication this conference could not have been such a success. The Quark Matter 2006 conference has received substantial financial support from many organizations, including the Chinese Academy of Sciences (CAS), the National Natural Science Foundation of China (NSFC), the Ministry of Education of China (MOE), Shanghai Science and Technology Committee (SSTC), Chinese Nuclear Physics Society (CNPS), Shanghai Institute of Applied Physics (SINAP), Central China Normal University (CCNU), China Center of Advanced Science and Technology (CCAST), Brookhaven National Laboratory (BNL), Lawrence Berkeley National Laboratory (LBNL), European Laboratory for Particle Physics (CERN), and Journal of Physics G: Nuclear and Particle Physics (IOP Publishing).

  16. PREFACE: Dynamics of wetting Dynamics of wetting

    Science.gov (United States)

    Grest, Gary S.; Oshanin, Gleb; Webb, Edmund B., III

    2009-11-01

    dynamics and the broader field of fluid dynamics [7-9]. Such an active field requires an occasional collective examination of current research to highlight both recent successes and remaining challenges. Herein, we have collected a range of articles to illustrate the broad nature of research associated with understanding dynamics of moving condensed matter three phase contact lines. Despite the breadth of topics examined, certain unifying themes emerge. The role of the substrate surface is critical in determining kinetics of wetting; this is evidenced by the attention given to this in articles herein. McHale et al investigate the role of surface topography on wetting kinetics and how its effect can be incorporated in existing theories describing contact line dynamics. Moosavi et al examine surface topography effects via a mesoscopic hydrodynamics approach. The capillary driven motion of fluid through structures on a surface bears tremendous importance for microfluidics studies and the emerging field of nanofluidics. Blow et al examine this phenomena for liquid imbibition into a geometric array of structures on a solid surface, while Shen et al analyze the effects of surface temperature during boiling and non-boiling conditionson droplet impingement dynamics. Finally, Pesika et al discover a wonderful world of smart surfaces, like gecko adhesion pads. A number of papers utilize computational modeling to explore phenomena underlying wetting dynamics and to consider relevant mechanisms in terms of existing theory for contact line dynamics. Winter et al utilize Monte Carlo simulation techniques and thermodynamic integration methods to test classical theory describing heterogeneous nucleation at a wall near a wetting transition. Qian et al briefly review the Onsager principle of minimum energy dissipation underlying many descriptions of dissipative systems; they then provide a variational approach description of hydrodynamics of moving contact lines and demonstrate the validity

  17. Fresh Water River discharges as observed by SMOS in the Arabian Sea and the Bay of Bengal

    Science.gov (United States)

    Olmedo, Estrella; Ballabrera-Poy, Joaquim; Turiel, Antonio

    2017-04-01

    filtering criteria is implemented, SMOS provides coherent SSS measurements close to the coast, and especially in these regions of the Indian Ocean, providing near real-time information suitable for validation and ocean data assimilation. References: [1] Font, J., Camps, A., Borges, A., Martin-Neira, M., Boutin, J., Reul, N., Kerr, Y., Hahne, A., and Mechlenburg, S. (2010). SMOS: the challenging sea surface salinity measurement from space. Proceedings of the IEEE, 98:649. [2] Olmedo, E., Martínez, J., Turiel, A., Ballabrera-Poy, J., and Portabella, M., (2017), "Debiased Non-Bayesian retrieval: a novel approach to SMOS Sea Surface Salinity, Remote Sensing of Environment, under review. [3] Zweng, M.M, J.R. Reagan, J.I. Antonov, R.A. Locarnini, A.V. Mishonov, T.P. Boyer, H.E. Garcia, O.K. Baranova, D.R. Johnson, D.Seidov, M.M. Biddle, 2013. World Ocean Atlas 2013, Volume 2: Salinity. S. Levitus, Ed., A. Mishonov Technical Ed.; NOAA Atlas NESDIS 74, 39 pp [4] Dai, A., and K. E. Trenberth, (2002): Estimates of freshwater discharge from continents: Latitudinal and seasonal variations. J. Hydrometeorol., 3, 660-687 [5] Dai, A., T. Qian, K. E. Trenberth, and J. D Milliman, (2009): Changes in continental freshwater discharge from 1949-2004. J. Climate, 22, 10, 2773-2792 [6] Bonjean F. and G.S.E. Lagerloef, (2002): Diagnostic model and analysis of the surface currents in the tropical Pacific ocean, J. Phys. Oceanogr., 32, 2,938-2,954 [7] Donlon, C. J., M. Martin, J. D. Stark, J. Roberts-Jones, E. Fiedler and W. Wimmer, (2011). The perational Sea Surface Temperature and Sea Ice analysis (OSTIA). Remote Sensing of the Environment. doi: 10.1016/j.rse.2010.10.017 2011. [8] Martin, M.J., A. Hines and M.J. Bell, (2007). Data assimilation in the FOAM operational short-range ocean forecasting system: a description of the scheme and its impact. Q.J.R. Meteorol. Soc., 133:981-995. [9] John D. Stark, Craig J. Donlon, Matthew J. Martin and Michael E. McCulloch, (2007), OSTIA : An operational

  18. Abstracts from the 15th International Myopia Conference

    Directory of Open Access Journals (Sweden)

    Alexandra Benavente-Perez

    2016-11-01

    against form deprivation myopia in chickens, by local signaling within the retina Ladan Ghodsi, William K. Stell O14 Contributions of ON and OFF pathways to emmetropization and form deprivation myopia in mice Machelle T. Pardue, Ranjay Chakraborty, Han na Park, Curran S. Sidhu, P. Michael Iuvone O15 Response of the human choroid to defocus Michael J Collins O16 What can RNA sequencing tell us about myopic sclera? Nethrajeith Srinvasalu, Sally A McFadden, Paul N Baird O17 Overview of dopamine, retinal function, and myopia P. Michael Iuvone O18 The eye as a "robust" optical system and myopia Pablo Artal O19 Effect of discontinuation of orthokeratology lens wear on axial elongation in children Pauline Cho, SW Cheung O20 Myopia prevention in Taiwan Pei-Chang Wu O21 Alternatives to ultraviolet light and riboflavin for in vivo crosslinking of scleral collagen Quan V. Hoang, Sally A. McFadden O22 Absence of intrinsically photosensitive retinal ganglion cells (ipRGC alters normal refractive development in mice Ranjay Chakraborty, Duk C. Lee, Erica G. Landis, Michael A. Bergen, Curran Sidhu, Samer Hattar, P. Michael Iuvone, Richard A. Stone, Machelle T. Pardue O23 Scleral micro-RNAs in myopia development and their potential as therapeutic targets Ravi Metlapally O24 Effects of the long-wavelength filtered continuous spectrum on emmetropization in juvenile guinea pigs Ruiqin Li, Qinglin Xu, Hong Zhon, Chenglin Pan, Weizhon Lan, Xiaoning Li, Ling Chen, Zhikuan Yang O25 Ocular and environmental factors associated with eye growth in childhood Scott A. Read O26 Overview- prevention and prediction of myopia and pathologic myopia Seang-Mei Saw O27 New insights into the roles of retinal dopamine in form-deprivation myopia and refractive development in C57BL/6 mice Shi-Jun Weng, Xiao-Hua Wu, Kang-Wei Qian, Yun-Yun Li, Guo-Zhong Xu, Furong Huang, Xiangtian Zhou, Jia Qu, Xiong-Li Yang, Yong-Mei Zhong O28 The effects of the adenosine antagonist, 7-methylxanthine, on refractive development in

  19. Documenting the Earliest Chinese Journals

    Directory of Open Access Journals (Sweden)

    Jian-zhong (Joe Zhou

    2001-10-01

    Full Text Available

    頁次:19-24

    According to various authoritative sources, the English word "journal" was first used in the 16lh century, but the existence of the journal in its original meaning as a daily record can be traced back to Acta Diuma (Daily Events in ancient Roman cities as early as 59 B.C. This article documents the first appearance of Chinese daily records that were much early than 59 B.C.

    The evidence of the earlier Chinese daily records came from some important archaeological discoveries in the 1970's, but they were also documented by Sima Qian (145 B.C. - 85 B.C., the grand historian of the Han Dynasty imperial court. Sima's lifetime contribution was the publication of Shi Ji (史記 (The Grand Scribe's Records, the Records hereafter. The Records is a book of history of a grand scope. It encompasses all Chinese history from 30lh century B.C. through the end of the second century B.C. in 130 chapters and over 525,000 Chinese

  20. Radiotracers For Lipid Signaling Pathways In Biological Systems

    Energy Technology Data Exchange (ETDEWEB)

    Gatley, S. J. [Northeastern Univ., Boston, MA (United States)

    2016-09-26

    enzymes such as fatty acid amide hydrolase, which may regulate endocannabinoid tone in animals. Early results were presented at the 2011 ICRS meeting, and at the 2012 Society for Neurosciences. Narachidonoylethanolamine is an endocannabinoid signaling messenger in animals and is known as “anandamide”. It is one of several families of signaling molecules derived from arachidonic acid, the principal omega-6 polyunsaturated fatty acids (PUFA’s) in animal species. Other derivatives of arachidonic acid include thromboxanes and prostaglandins. Full details of the studies with the ethanolamide isotopomers were a part of the PhD dissertation of Kun Hu (nee Qian), and were submitted for publication to Nuclear Medicine and Biology in August 2016. Syntheses of [14C]docosahexanoylethanolamine isotopomers and preliminary biological investigations Docosahexaenoic acid (DHA) is the omega-3 PUFA that can be regarded in some respects as the counterpart of arachidonic acid in the omega-6 series. While arachidonic acid is proinflammatory, DHA is anti-inflammatory, and foods high in DHA (or artificially enriched in DHA) are commonly regarded as promoting health. In contrast to the large literature on the Nethanolamide of arachidonic acid (i.e. the endocannabinoid anandamide) as of now (9/25/2016) there are only six papers on the corresponding ethanolamide of DHA, and when our studies under this grant began there were none. Beneficial actions of endogenously produced DHAethanolamine (“synaptamide”) have been indicated, and to help elucidate the possible roles of synaptamide, we have synthesized this molecule for the first time labeled with C-14 in either the ethanolamine moiety or the fatty acid moiety. Studies of the disposition of endogenously administered isotopomers of DHA-ethanolamine are in progress, to complement tissue culture experiments evaluation hypothesized protective effects of this DHA derivative.

  1. Literature Review of Bozhou Tusi Studies (2001-2013)

    Institute of Scientific and Technical Information of China (English)

    LI Yueping

    2014-01-01

    education .Education developed in the Tusi are-as in Bozhou.As a result of this, the cultural qualities of the people was raised and flourished;the development of literature and art in the north-ern part of Qian ( Guizhou ) was promoted , and the rule and control of the central government in Bozhou had been consolidated .By doing so , na-tional unity had been maintained and social civili-zation and progress in the ethnic minority areas of western part in China had been promoted . 3.Tusi Graves Graves were not only places for burying dead bodies, but also were places for embodying social etiquette, religion and beliefs, customs of burial, and architectural arts .Graves are very important historical cultural relics .The graves of the Yangs had unique value as cultural relics .They recorded social development and wars on the old special place at that time;and recorded a family's rise and fall in history .All of the things in the graves , such as gravestones , headstones and epitaphs , have im-portant academic value , and are very important for the people who study the families of the Yang Tusi. To some extent , the graves of the Yangs , with their grand scale and exquisite construction , embodied the development of culture and economy in Bozhou . IV.Deficiencies in the Studies Although , there are many articles about Bozhou Tusi studies in 2001-2013, especially in 2012 -2013 , we can find some deficiencies in these studie 1.The definition is not certain .Which Tusi lasted the longest in the border area , Tianshi Tusi or Yang Tusi? 2 .There is a lack of studies on the relevant historical cultural relics , such as Sanyuanshan , Paotaipo etc . around Hailongtun . This gap will need to be filled in . 3 .There are not enough important study re-sults which have impact .Over 85% study results have been published in journals in Guizhou , and about 80% have been published in common jour-nals, which indicates that the impact of study re-sults needs to be raised . 4 .The number of

  2. Advanced Materials and Processing 2010

    Science.gov (United States)

    Zhang, Yunfeng; Su, Chun Wei; Xia, Hui; Xiao, Pengfei

    2011-06-01

    EuFe[symbol] materials / Huen Kan ... [et al.] -- Synthesis and characterization of Ni(OH)[symbol] nanosheets by a simple route at low temperature / Qian Li ... [et al.] -- The prediction of laser clad parameters based on neural network / Jichang Liu and Libin Ni -- The effects of Y[symbol] doping on the phase structure and photoluminescence properties of (Gd[symbol]) red phosphors / Qi Zhu ... [et al.] -- Design of an in situ detection system for laser hardened width / Caixia Yang and Jichang Liu -- Numerical simulation microstructure morphology evolution and solute microsegregation of Al-Si-Cu ternary alloys during solidification process / Shuisheng Xie ... [et al.].A shear-lag model for carbon nonotube-reinforced magnesium matrix composites / Wei-Xue Li ... [et al.] -- Corrosion behavior of the Alumina Coated Al6061 Alloy by Plasma electrolytic oxidation / Kai Wang ... [et al.] -- A simple route for synthesis of tin dioxide nanorods based on improved solid-state reactions / Yuehua Li ... [et al.] -- Comparative study on microstructure and magnetic properties of amorphous wires with different diameters / Jing-Shun Liu ... [et al.] -- Chemical-Vapor-Depositing (CVD) aluminium film on steel surface with the disproportionation reaction of Al[symbol]S / Wu Guoyuan and Dai Yongnian -- The microstructure and properties of super martensitic stainless steel microalloyed with tungsten and copper / Dong Ye ... [et al.] -- Design of low elastic modulus Ti-Nb-Zr alloys for implant materials / Xiping Song ... [et al.] -- In situ monitoring molten pool parameters for detecting visible defects in laser cladding / Liusha Yang ... [et al.].

  3. PREFACE: Preface

    Science.gov (United States)

    Luo, Qingming; Wang, Lihong V.; Tuchin, Valery V.

    2011-02-01

    Centre (Canada)Gang Zheng, University of Toronto (Canada)Dongping Zhong, The Ohio State University (USA) Organizing CommitteeLing Fu (Chair), Huazhong University of Science and Technology (China)Yuandi Zhao (Chair), Huazhong University of Science and Technology (China)Hui Gong, Huazhong University of Science and Technology (China)Pengcheng Li, Huazhong University of Science and Technology (China)Bifeng Liu, Huazhong University of Science and Technology (China)Qian Liu, Huazhong University of Science and Technology (China)Shaoqun Zeng, Huazhong University of Science and Technology (China)Zhihong Zhang, Huazhong University of Science and Technology (China)Dan Zhu, Huazhong University of Science and Technology (China) Local SecretariatHua Shi, Huazhong University of Science and Technology (China)

  4. EDITORIAL: Focus on Iron-Based Superconductors FOCUS ON IRON-BASED SUPERCONDUCTORS

    Science.gov (United States)

    Hosono, Hideo; Ren, Zhi-An

    2009-02-01

    Tegel, Inga Schellenberg, Falko M Schappacher, Rainer Pöttgen, Joachim Deisenhofer, Axel Günther, Florian Schrettle, Alois Loidl and Dirk Johrendt The superconductor KxSr1-xFe2As2: normal state and superconducting properties B Lv, M Gooch, B Lorenz, F Chen, A M Guloy and C W Chu Effect of 3d transition metal doping on the superconductivity in quaternary fluoroarsenide CaFeAsF Satoru Matsuishi, Yasunori Inoue, Takatoshi Nomura, Youichi Kamihara, Masahiro Hirano and Hideo Hosono Influence of the rare-earth element on the effects of the structural and magnetic phase transitions in CeFeAsO, PrFeAsO and NdFeAsO Michael A McGuire, Raphaël P Hermann, Athena S Sefat, Brian C Sales, Rongying Jin, David Mandrus, Fernande Grandjean and Gary J Long Heat capacity measurements on FeAs-based compounds: a thermodynamic probe of electronic and magnetic states P J Baker, S R Giblin, F L Pratt, R H Liu, G Wu, X H Chen, M J Pitcher, D R Parker, S J Clarke and S J Blundell Spin fluctuations, interband coupling and unconventional pairing in iron-based superconductors Zi-Jian Yao, Jian-Xin Li and Z D Wang Superconductivity induced by Ni doping in BaFe2As2 single crystals L J Li, Y K Luo, Q B Wang, H Chen, Z Ren, Q Tao, Y K Li, X Lin, M He, Z W Zhu, G H Cao and Z A Xu Metamagnetic transition in EuFe2As2 single crystals Shuai Jiang, Yongkang Luo, Zhi Ren, Zengwei Zhu, Cao Wang, Xiangfan Xu, Qian Tao, Guanghan Cao and Zhu'an Xu Pressure dependence of the thermoelectric power of the iron-based high-Tc superconductor SmFeAsO0.85 N Kang, P Auban-Senzier, C R Pasquier, Z A Ren, J Yang, G C Che and Z X Zhao Superconductivity in some heavy rare-earth iron arsenide REFeAsO1-δ (RE = Ho, Y, Dy and Tb) compounds Jie Yang, Xiao-Li Shen, Wei Lu, Wei Yi, Zheng-Cai Li, Zhi-An Ren, Guang-Can Che, Xiao-Li Dong, Li-Ling Sun, Fang Zhou and Zhong-Xian Zhao The delicate electronic and magnetic structure of the LaFePnO system (Pn = pnicogen) S Lebègue, Z P Yin and W E Pickett

  5. First Announcement and Call for Papers SECOND INTERNATIONAL SYMPOSIUM ON FLOOD DEFENCE

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    as follows:-History of flood defence -Hydrology and precipitation -Flood modeling -Pollution and disease pathogens -Flood plain and flood plain management -Disasters induced by sedimentation and erosion -Landslide and debris flow -Reservoir and reservoir management -Environmental impact of floods -Urban drainage system -Global climate modeling -Coastal floods and storm surges -New development in flood prevention -Drainage management -Real time control of flooding -Strategies/concepts on flood defence -Laws and policies on flood control -Experience and practices in flood control -Flood insurance -Hydro-informatics -Engineering measures mitigating flooding disasters CALL FOR PAPERS Papers are invited on the topics indicated and others falling within the scope of the conference. The official language of the conference is English, so papers and all correspondence are required in English. Two copies of abstract of about 400 words, clearly stating the purpose, results and conclusions of the work to be presented in the full paper should be submitted to the Local Organizing Committee (LOC) before 31 Dec. 2001 for review. Each abstract should clearly state the most relevant conference topics. The full-length paper is limited within 6-8 pages. LOC will forward simple typing instructions for preparing papers to the authors together with the notification of acceptance of their abstracts. No camera-ready manuscripts but a word-file of the paper (including all tables and figures) are required.The LOC will edit and print all papers in an identical format before sending for press. The proceedings will be published by an international press house.ADVISORY COMMITTEE Chairpersons QIAN Zheng-ying (Vice president of Chinese People's Political Consultative Conference) YANG Zhen-Huai (Ex-Minister of Water Resources of China)Alam, S. France Petry, Bela Netherlands Cao, Zhengqi China Plate, E.J. Germany Chen, C.J. USA Rodi, W. Germany Dai, Dingzhong China Saad, M.B.A. Egytp Di Silvio, G

  6. Poster Session B

    Science.gov (United States)

    2014-01-01

    peptide retention time trainer kit and analyzed using unbiased data-dependent acquisition (DDA) method. Initial characterization experiments using unbiased DDA facilitated the building of detailed murine cardiac tissue spectral library. The spectral library records contain the relative retention time information based on the standard peptides as well as highly confident endogenous peptides, precursor and product ion information such as measured mass values and relative abundance used to create a consensus product ion spectrum. The spectral library information was used to create reference information to perform qual/quan determination in real-time. The pSMART method was used to acquire qualitative/ quantitative data analysis using one HR/AM MS and a series of narrow DIA mass windows. Our pSMART strategy resulted in 30% more peptide identifications per run than a standard DDA run. Additionally, using pSMART, we were able to confirm MS1 quantitation at low abundance levels with MS/MS for each peptide. This novel acquisition enabled quantitation of previously identified peptides as well as novel putative targets of aging. By identifying and quantifying more targets, we were able to better characterize the dynamic proteomic changes of cardio-dysfunction in aging mice. B.10 Quantitative Site-Specific Profiling S-glutathionylation in Macrophages in Response to Engineered Nanomaterial-induced Oxidative Stress Jicheng Duan, Vamsi K. Kodali, Matthew J. Gaffrey, Jia Guo, Rosalie K. Chu, David G. Camp, Richard D. Smith, Brian Thrall, Wei-Jun Qian Pacific Northwest National Laboratory, Richland, WA, USA Engineered nanoparticles are emerging functional materials with unique physicochemical properties, which make them desirable for commercial and medical applications. It is important to assess the toxicity of nanomaterials and recognize the underlying mechanisms of their toxicity. Oxidative stress is known to play important roles in nanomaterial-induced cellular toxicity, which leads to