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Sample records for puten meleagris gallopavo

  1. Reticuloendotheliosis in a wild turkey (Meleagris gallopavo) from coastal Georgia.

    Science.gov (United States)

    Hayes, L E; Langheinrich, K A; Witter, R L

    1992-01-01

    An emaciated wild turkey (Meleagris gallopavo) exhibiting neurologic signs was found on Ossabaw Island, Chatham County, Georgia (USA) on 11 April 1989. The neurologic abnormalities observed included ataxia, drooping wings, head tremors, torticollis, and circling. At necropsy, discrete yellowish-white nodules, varying in size from 2 to 5 mm, were present in the spleen. White nodular lesions approximately 2 mm in diameter were observed beneath the mucosal surface of the distal esophagus. Histopathologic examination of the splenic nodules disclosed large numbers of primitive lymphoreticular cells with leptochromatic nuclei and abundant, slightly basophilic cytoplasms. The mitotic index in these cells was moderate to high. Similar neoplastic cells composed the masses observed in the esophagus. Multifocal, mild perivascular cuffing with mononuclear cells was found in the lumbar spinal cord, brain, and brain stem. Reticuloendotheliosis virus, subtype 3, was isolated from samples of the spleen and liver.

  2. Feeding ecology of Merriam's turkeys (Meleagris gallopavo merriami) in the Black Hills, South Dakota

    Science.gov (United States)

    Mark A. Rumble; Stanley H. Anderson

    1996-01-01

    W e studied the feeding ecology of Merriam’s turkey (Meleagris gallopavo merriami) in the Black Hills, South Dakota, between 1986 and 1989. Adult birds consumed 78 kinds of food, of which four food categories constituted >79% of winter diets and six food categories constituted >75% of summer diets. Ponderosa pine (Pinus ponderosa) seeds were...

  3. Habitat selection of Merriam's turkey (Meleagris gallopavo Merriami) hens with poults in the Black Hills, South Dakota

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    Mark A. Rumble; Stanley H. Anderson

    1993-01-01

    We studied habitat selection patterns of Merriam's Turkey (Meleagris gallopavo merriami) hens with poults in a ponderosa pine (Pinus ponderosa) ecosystem. Thirty-six radio-marked hens produced 19 broods, and we obtained 230 locations of hens with poults. We described vegetation of habitats using criteria from the Rocky...

  4. Serological and microbial survey of Mycoplasma gallisepticum in wild turkeys (Meleagris gallopavo) from six western states.

    Science.gov (United States)

    Fritz, B A; Thomas, C B; Yuill, T M

    1992-01-01

    From 1986 to 1989, sera from wild turkeys (Meleagris gallopavo), including three subspecies (M. gallopavo intermedia, M. gallopavo merriami and M. gallopavo mexicana) trapped in six western states were tested for antibody to Mycoplasma gallisepticum (MG) (n = 724), M. synoviae (MS) (n = 461) and M. meleagridis (MM) (n = 354) using the rapid plate agglutination (RPA) assay. Subsamples of these sera were also evaluated using the hemagglutination inhibition (HI) assay for antibody to MG (n = 664) and MS (n = 403). Attempts were made to isolate mycoplasmas by swabbing the trachea and cloaca of 190 live wild turkeys and from various tissues (sinus, nasal turbinates, trachea, lung, ovaries and oviduct) from 76 turkeys at necropsy. Isolates were identified using an immunobinding assay. Seroprevalence of MG, MS and MM in the RPA test was highly variable among years and geographic sites, ranging from 0 to 85%, 0 to 87%, and 0 to 83%, respectively, for each mycoplasma species. Of the 724 wild turkey sera tested, 200 (28%) were positive using the RPA assay, while only 20 (3%) of 664 sera tested using the HI assay were positive (at a titer greater than/= 1:80) for antibody to MG. Of the 461 sera tested 178 (39%) were RPA positive for MS, whereas none of the 403 samples tested by HI were positive for MS. Antibody to MM was detected in 72 (20%) of 354 turkey sera tested by RPA. Mycoplasmas were cultured from 81 (30%) of 266 wild turkeys, including 48 that were sampled live and 33 that were examined by necropsy. Mycoplasmas were isolated from every population in which culture was attempted. M. gallopavonis (MGP) was isolated from 37 (46%) of 81 birds which yielded mycoplasma, representing seven of 12 populations sampled. MG was isolated from lower respiratory tissues of one Rio Grande wild turkey trapped in Texas. M. synoviae was isolated from five of 16 Merriam's wild turkeys trapped in Arizona. Sera of birds from which MG or MS was isolated were positive to the respective

  5. Caracterización de sitios de percha del guajolote silvestre (Meleagris gallopavo mexicana) en Sierra Fría, Aguascalientes, México Roost sites characteristics of wild turkey (Meleagris gallopavo mexicana) in Sierra Fria, Aguascalientes, Mexico

    OpenAIRE

    Marcelo Márquez-Olivas; Edmundo García-Moya; Carlos González-Rebeles Islas; Humberto Vaquera-Huerta

    2007-01-01

    Entre octubre y noviembre del año 2001 y mayo de 2002 se localizaron 16 sitios de percha de guajolote silvestre (Meleagris gallopavo mexicana) en la Sierra Fría de Aguascalientes. El 87.5% de los sitios se encontraron en bosques de pino-encino (en rodales de pinos), en altitudes de 2500 a 2650 m. En la parte alta (2/3) de la cuesta se localizaron 8 de los sitios y 7 sobre laderas de exposición noreste, en una pendiente promedio de 38.9 ± 12.6%. El 62.5% de los sitios se usaron durante todo el...

  6. Evaluation of Cytology for Diagnosing Avian Pox in Wild Turkeys ( Meleagris gallopavo).

    Science.gov (United States)

    Hydock, Kira; Brown, Holly; Nemeth, Nicole; Poulson, Rebecca; Casalena, Mary Jo; Johnson, Joshua B; Brown, Justin

    2018-03-01

    Avian pox virus is a common cause of proliferative skin disease in wild turkeys ( Meleagris gallopavo); however, other etiologies may produce grossly indistinguishable lesions. Common methods for diagnosing avian pox include histopathology, virus isolation, and PCR. While these methods are sufficient in most cases, each has their limitations. Cytology is a cost-effective and rapid approach that may be useful when traditional diagnostics are not feasible. The objective of this study was to evaluate the performance of cytology relative to histopathology and PCR for avian pox diagnosis in wild turkeys. Fifty wild turkeys were submitted for necropsy due to nodular skin lesions on unfeathered skin of the head. Of these, five had similar skin lesions on the unfeathered legs and 26 had plaques on the mucosa of the oropharynx or esophagus. Representative skin, oropharyngeal, and esophageal lesions from all birds were examined with cytology and histopathology. Skin lesions on the head of each bird were also tested for avian pox virus via PCR. Histopathology and PCR were equally sensitive in diagnosing avian pox from skin lesions on the head. There were no significant differences between cytologic and histopathologic diagnosis of avian pox from skin lesions on the head (sensitivity = 97.4%, specificity = 100.0%), legs (sensitivity = 75.0%, specificity = 100.0%), or from lesions in the oropharynx and esophagus (sensitivity of 62.5%). Similarly, there were no significant differences between PCR and cytology for diagnosis of pox viral skin lesions of the head. Relative to PCR detection of avian pox virus, cytology had a sensitivity of 90.0% and a specificity of 90.0%. These results suggest that cytology is a useful tool for diagnosis of avian pox in wild turkeys.

  7. Serum miRNA disregulation during transport-related stress in turkey (Meleagris gallopavo

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    Andreia Tomás Marques

    2015-07-01

    Full Text Available MicroRNAs (miRNAs are small 21-25 nucleotide regulatory non-coding RNAs that modulate gene expression in eukaryotic organisms. miRNAs are complementary to the 3′-untranslated regions of mRNA and act as post-transcriptional regulators of gene expression, exhibiting remarkable stability in extracellular fluids such as blood. Turkey (Meleagris gallopavo farming is a species economically relevant but the lack of efficient protocols for the evaluation of commercial turkeys prevents to measure the impact of industry practices on birds productivity and welfare. In order to identify potential molecular biomarkers for monitoring stress in turkey’s handling, we investigated by TaqMan qPCR the abundance of five circulating miRNA, namely miR-22, miR-155, miR-181a, miR-204 and miR-365, previously demonstrated to be involved in stress in chicken due to feed deprivation. Road transportation related procedures were selected as stressful model for this study. The serum of twenty healthy animals was collected before and after 2h transportation. Our results demonstrated that miR-22, miR-155 and miR-365 are statistically more expressed after road transportation. Receiver-operator characteristics (ROC analysis was used to estimate the diagnostic value of these miRNAs to evaluate the stress in animals. The serum level of miR-22, miR-155 and miR-365 can discriminate stressed from non-stressed animals with an AUC=0.763, 0.710 and 0.704, respectively, and the average expression of their combination has the same specificity (AUC=0.745. miR-22, miR-155 and miR-365 are stress-specific markers and can be considered as suitable biomarkers to identify turkeys stressed by road transportation.

  8. Whole genome SNP discovery and analysis of genetic diversity in Turkey (Meleagris gallopavo)

    Science.gov (United States)

    2012-01-01

    Background The turkey (Meleagris gallopavo) is an important agricultural species and the second largest contributor to the world’s poultry meat production. Genetic improvement is attributed largely to selective breeding programs that rely on highly heritable phenotypic traits, such as body size and breast muscle development. Commercial breeding with small effective population sizes and epistasis can result in loss of genetic diversity, which in turn can lead to reduced individual fitness and reduced response to selection. The presence of genomic diversity in domestic livestock species therefore, is of great importance and a prerequisite for rapid and accurate genetic improvement of selected breeds in various environments, as well as to facilitate rapid adaptation to potential changes in breeding goals. Genomic selection requires a large number of genetic markers such as e.g. single nucleotide polymorphisms (SNPs) the most abundant source of genetic variation within the genome. Results Alignment of next generation sequencing data of 32 individual turkeys from different populations was used for the discovery of 5.49 million SNPs, which subsequently were used for the analysis of genetic diversity among the different populations. All of the commercial lines branched from a single node relative to the heritage varieties and the South Mexican turkey population. Heterozygosity of all individuals from the different turkey populations ranged from 0.17-2.73 SNPs/Kb, while heterozygosity of populations ranged from 0.73-1.64 SNPs/Kb. The average frequency of heterozygous SNPs in individual turkeys was 1.07 SNPs/Kb. Five genomic regions with very low nucleotide variation were identified in domestic turkeys that showed state of fixation towards alleles different than wild alleles. Conclusion The turkey genome is much less diverse with a relatively low frequency of heterozygous SNPs as compared to other livestock species like chicken and pig. The whole genome SNP discovery

  9. Whole genome SNP discovery and analysis of genetic diversity in Turkey (Meleagris gallopavo

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    Aslam Muhammad L

    2012-08-01

    Full Text Available Abstract Background The turkey (Meleagris gallopavo is an important agricultural species and the second largest contributor to the world’s poultry meat production. Genetic improvement is attributed largely to selective breeding programs that rely on highly heritable phenotypic traits, such as body size and breast muscle development. Commercial breeding with small effective population sizes and epistasis can result in loss of genetic diversity, which in turn can lead to reduced individual fitness and reduced response to selection. The presence of genomic diversity in domestic livestock species therefore, is of great importance and a prerequisite for rapid and accurate genetic improvement of selected breeds in various environments, as well as to facilitate rapid adaptation to potential changes in breeding goals. Genomic selection requires a large number of genetic markers such as e.g. single nucleotide polymorphisms (SNPs the most abundant source of genetic variation within the genome. Results Alignment of next generation sequencing data of 32 individual turkeys from different populations was used for the discovery of 5.49 million SNPs, which subsequently were used for the analysis of genetic diversity among the different populations. All of the commercial lines branched from a single node relative to the heritage varieties and the South Mexican turkey population. Heterozygosity of all individuals from the different turkey populations ranged from 0.17-2.73 SNPs/Kb, while heterozygosity of populations ranged from 0.73-1.64 SNPs/Kb. The average frequency of heterozygous SNPs in individual turkeys was 1.07 SNPs/Kb. Five genomic regions with very low nucleotide variation were identified in domestic turkeys that showed state of fixation towards alleles different than wild alleles. Conclusion The turkey genome is much less diverse with a relatively low frequency of heterozygous SNPs as compared to other livestock species like chicken and pig. The

  10. Breed-Specific Haematologic Reference Values in Adult Turkeys (Meleagris gallopavo in the Humid Tropics of Nigeria

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    Daniel-Igwe G.

    2017-03-01

    Full Text Available One hundred (50 males and 50 females B-not strain indigenous turkeys, Meleagris gallopavo, were used to determine the reference values for their haematological parameters. The turkeys were housed in the poultry unit and jugular venepunctures were used to collect their blood. The haematological parameters were determined using standard procedures. The mean values of: the packed cell volume (PCV; 37.29 ± 0.37 %, red blood cell (RBC counts (2.50 ± 0.44 × 106.µl−1, haemoglobin concentration (Hbc; 10.89 ± 0.34 g.dl−1, mean corpuscular volume (MCV; 150.63 ± 0.73 fl, mean corpuscular haemoglobin (MCH; 44.29 ± 1.78 pg, mean corpuscular haemoglobin concentration (MCHC; 29.10 ± 0.73 g.dl−1, and white blood cell (WBC counts (12.41 ± 0.83 × 103 µl−1 were determined. No significant differences were found between the male and female B-not strain turkeys in this study. The results will help in the interpretation of cases of disease when there are variations in the values and serve as baseline data for B-not strain of turkeys in the humid tropics.

  11. Multi-platform next-generation sequencing of the domestic turkey (Meleagris gallopavo: genome assembly and analysis.

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    Rami A Dalloul

    2010-09-01

    Full Text Available A synergistic combination of two next-generation sequencing platforms with a detailed comparative BAC physical contig map provided a cost-effective assembly of the genome sequence of the domestic turkey (Meleagris gallopavo. Heterozygosity of the sequenced source genome allowed discovery of more than 600,000 high quality single nucleotide variants. Despite this heterozygosity, the current genome assembly (∼1.1 Gb includes 917 Mb of sequence assigned to specific turkey chromosomes. Annotation identified nearly 16,000 genes, with 15,093 recognized as protein coding and 611 as non-coding RNA genes. Comparative analysis of the turkey, chicken, and zebra finch genomes, and comparing avian to mammalian species, supports the characteristic stability of avian genomes and identifies genes unique to the avian lineage. Clear differences are seen in number and variety of genes of the avian immune system where expansions and novel genes are less frequent than examples of gene loss. The turkey genome sequence provides resources to further understand the evolution of vertebrate genomes and genetic variation underlying economically important quantitative traits in poultry. This integrated approach may be a model for providing both gene and chromosome level assemblies of other species with agricultural, ecological, and evolutionary interest.

  12. Earliest Mexican Turkeys (Meleagris gallopavo) in the Maya Region: implications for pre-Hispanic animal trade and the timing of turkey domestication.

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    Thornton, Erin Kennedy; Emery, Kitty F; Steadman, David W; Speller, Camilla; Matheny, Ray; Yang, Dongya

    2012-01-01

    Late Preclassic (300 BC-AD 100) turkey remains identified at the archaeological site of El Mirador (Petén, Guatemala) represent the earliest evidence of the Mexican turkey (Meleagris gallopavo) in the ancient Maya world. Archaeological, zooarchaeological, and ancient DNA evidence combine to confirm the identification and context. The natural pre-Hispanic range of the Mexican turkey does not extend south of central Mexico, making the species non-local to the Maya area where another species, the ocellated turkey (Meleagris ocellata), is indigenous. Prior to this discovery, the earliest evidence of M. gallopavo in the Maya area dated to approximately one thousand years later. The El Mirador specimens therefore represent previously unrecorded Preclassic exchange of animals from northern Mesoamerica to the Maya cultural region. As the earliest evidence of M. gallopavo found outside its natural geographic range, the El Mirador turkeys also represent the earliest indirect evidence for Mesoamerican turkey rearing or domestication. The presence of male, female and sub-adult turkeys, and reduced flight morphology further suggests that the El Mirador turkeys were raised in captivity. This supports an argument for the origins of turkey husbandry or at least captive rearing in the Preclassic.

  13. Caracterización de sitios de percha del guajolote silvestre (Meleagris gallopavo mexicana en Sierra Fría, Aguascalientes, México Roost sites characteristics of wild turkey (Meleagris gallopavo mexicana in Sierra Fria, Aguascalientes, Mexico

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    Marcelo Márquez-Olivas

    2007-06-01

    Full Text Available Entre octubre y noviembre del año 2001 y mayo de 2002 se localizaron 16 sitios de percha de guajolote silvestre (Meleagris gallopavo mexicana en la Sierra Fría de Aguascalientes. El 87.5% de los sitios se encontraron en bosques de pino-encino (en rodales de pinos, en altitudes de 2500 a 2650 m. En la parte alta (2/3 de la cuesta se localizaron 8 de los sitios y 7 sobre laderas de exposición noreste, en una pendiente promedio de 38.9 ± 12.6%. El 62.5% de los sitios se usaron durante todo el periodo de estudio y el resto, de manera ocasional. La mayoría de los dormideros se encontraron cerca de comederos o cebaderos y de parcelas agrícolas. Se contabilizaron de 1 a 23 árboles de percha utilizados por sitio, distribuidos a una distancia promedio de 24.4 ± 17.8 metros. El 81.2% de los árboles (n=170 fueron pinos (Pinus teocote, P. durangensis, P. leiophylla y P. michoacana, 17.6% encinos (Quercus laurina, Q. grisea y Q. eduardii y 1.2% madroños (Arbutus arizonica. La altura promedio de los árboles fue de 16.6 ± 4.4 m y el diámetro 43.2 ± 11.5 cm. La preferencia del guajolote silvestre por utilizar pinos como dormideros en lugar de otras especies arbóreas, se debe posiblemente a que son árboles de mayor porte o a la conformación de su dosel; sin embargo, se ha observado que en lugares donde hay pocos pinos, los guajolotes utilizan para dormir otros árboles de características semejantes, como encinos, madroños o sicomoros.Sixteen roosting sites of Gould's wild turkey (Meleagris gallopavo mexicana were found between October and November of 2001 and May of 2002 in Sierra Fría, Aguascalientes. A large percentage (87.5% of the roost sites was located in oak-pine forest (in pine stands, at an elevation range of 2500 to 2650 meters. Eight roost sites were on the upper portion (2/3 of the ridge, and 7 at northeast exposures with an average slope of 38.9 ± 12.6%. Most (81.2% of the roost trees (n =170 were pines (Pinus teocote, P

  14. Systematization, distribution and territory of the caudal cerebral artery on the brain's surface of the turkey (Meleagris gallopavo

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    Amarílis Díaz de Carvalho

    2014-10-01

    Full Text Available Thirty Meleagris gallopavo heads with their neck segments were used. Animals were contained and euthanized with the association of mebezonium iodide, embutramide and tetracaine hydrochloride (T 61, Intervet by intravenous injection. The arterial system was rinsed with cold saline solution (15°C, with 5000IU heparin and filled with red-colored latex. The samples were fixed in 20% formaldehyde for seven days. The brains were removed with a segment of cervical spinal cord and after, the dura-mater was removed and the arteries dissected. The cerebral carotid arteries, after the intercarotid anastomosis, were projected around the hypophysis, until they reached the tuber cinereum and divided into their terminal branches, the caudal branch and the rostral branch. The rostral branch was projected rostrolateralwards and gave off, in sequence, two collateral branches, the caudal cerebral and the middle cerebral arteries and the terminal branch was as cerebroethmoidal artery. The caudal cerebral artery of one antimere formed the interhemispheric artery, which gave off dorsal hemispheric branches to the convex surface of both antimeres. Its dorsal tectal mesencephalic branch, of only one antimere, originated the dorsal cerebellar artery. In the interior of the cerebral transverse fissure, after the origin of the dorsal tectal mesencephalic artery, the caudal cerebral artery emitted occipital hemispheric branches, pineal branches and medial hemispheric branches, on both antimeres. The caudal cerebral artery's territory comprehended the entire surface of the dorsal hemioptic lobe, the rostral surface of the cerebellum, the diencephalic structures, the caudal pole and the medial surface of the cerebral hemisphere and in the convex surface, the sagittal eminence except for its most rostral third. Due to the asymmetry found in the caudal cerebral arteries' ramifications, the models were classified into three types and their respective subtypes.

  15. RISK FACTORS FOR AND SPATIAL DISTRIBUTION OF LYMPHOPROLIFERATIVE DISEASE VIRUS (LPDV) IN WILD TURKEYS (MELEAGRIS GALLOPAVO) IN NEW YORK STATE, USA.

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    Alger, Katrina; Bunting, Elizabeth; Schuler, Krysten; Whipps, Christopher M

    2017-07-01

    Lymphoproliferative disease virus (LPDV) is an oncogenic avian retrovirus that was previously thought to exclusively infect domestic turkeys but was recently shown to be widespread in Wild Turkeys ( Meleagris gallopavo ) throughout most of the eastern US. In commercial flocks, the virus spreads between birds housed in close quarters, but there is little information about potential risk factors for infection in wild birds. Initial studies focused on distribution of LPDV nationally, but investigation of state-level data is necessary to assess potential predictors of infection and detect patterns in disease prevalence and distribution. We tested wild turkey bone marrow samples (n=2,538) obtained from hunter-harvested birds in New York State from 2012 to 2014 for LPDV infection. Statewide prevalence for those 3 yr was 55% with a 95% confidence interval (CI) of 53-57%. We evaluated a suite of demographic, anthropogenic, and land cover characteristics with logistic regression to identify potential predictors for infection based on odds ratio (OR). Age (OR=0.16, 95% CI=0.13-0.19) and sex (OR=1.3, 95% CI=1.03-1.24) were strong predictors of LPDV infection, with juveniles less likely to test positive than adults, and females more likely to test positive than males. The number of birds released during the state's 40-yr translocation program (OR=0.993, 95% CI=0.990-0.997) and the ratio of agriculture to forest cover (OR=1.13, 95% CI=1.03-1.19) were also predictive of LPDV infection. Prevalence distribution was analyzed using dual kernel density smoothing to produce a risk surface map, combined with Kulldorff's spatial scan statistic and the Anselin Local Moran's I to identify statistically significant geographic clusters of high or low prevalence. These methods revealed the prevalence of LPDV was high (>50%) throughout New York State, with regions of variation and several significant clusters. We revealed new information about the risk factors and distribution of LPDV in New

  16. Sperm cell granuloma in a gobbler ( Meleagris Gallopavo ) | Ajayi ...

    African Journals Online (AJOL)

    Microscopically, there was severe diffuse testicular degeneration and necrosis of the germinal epithelial cells, extravasation of spermatozoa into the epididymal interstitium, inciting a granulomatous reaction with arteritis. Based on these findings, sperm cell granuloma was diagnosed. This is probably the first reported case ...

  17. Avian chlamydiosis in turkeys (Meleagris gallopavo in Bosnia and Herzegovina

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    Šatrović E.

    2012-01-01

    Full Text Available Chlamydiosis is a contagious disease of birds, mammals, reptiles and humans. So far it was demonstrated in 469 species of birds and among them, turkeys are the most susceptible domestic poultry species. The disease appears in epizootic form in intensive turkey farming. Since commercial poultry rearing is under-developed in Bosnia and Herzegovina, our investigation was based on extensively reared turkeys. Cloacal and oropharyngeal swabs were taken from 26 birds and infection was proven by common chlamydial LPS antigen detection tests (IDEIA and CW. We have used rRT-PCR technique targeting chlamydial ompA gene region in order to prove Chlamydia species. Five birds, (19.2% were found positive as judged by IDEIA and CW tests. Among them one was positive Cp. psittaci speciesspecific rRT-PCR, ompA gene.

  18. Characterization of proacrosin/acrosin system after liquid storage and cryopreservation of turkey semen (Meleagris gallopavo).

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    Słowińska, M; Liszewska, E; Dietrich, G J; Ciereszko, A

    2012-09-15

    This study was designed to identify the effect of liquid storage at 4 °C for 48 h and cryopreservation on the proacrosin/acrosin system of turkey spermatozoa. Anti-acrosin I antibodies were produced and used to demonstrate Western blot analysis profile of the proacrosin/acrosin system of sperm and seminal plasma and possible changes in the proacrosin/acrosin system of turkey sperm stored for 2.5, 24, and 48 h or cryopreserved. At the same time acrosin-like activity was examined by the measurement of amidase activity of sperm extracts, sperm suspension, and seminal plasma of turkey semen. A computer-assisted sperm analysis system was used to monitor the sperm motility characteristics of turkey sperm stored for 48 h or cryopreserved. Different profiles of the sperm proacrosin/acrosin system were observed regarding the presence or absence of inhibitors (p-nitrophenyl-p'-guanidine benzoate [NPGB] and Kazal family inhibitor) during the extraction process. When NPGB was present three main bands were observed with the molecular weight ranging from 66 to 35 kDa. Bands corresponding to acrosin I and II were not observed. In sperm extract without NPGB, three or four bands were observed with the molecular weight ranging from 41 to 30 kDa. The bands corresponding to acrosin I and II were observed. During liquid storage a decrease in sperm motility and an increase in sperm-extracted amidase activity were observed. After 24 and 48 h of storage, extracted amidase activity was higher than at 2.5 h by 24% and 31%, respectively. However, no changes in the Western blot analysis profiles of sperm extract and seminal plasma were visible during liquid storage. After cryopreservation a decrease in sperm motility and all sperm motility parameters were observed. In contrast to liquid storage, cryopreservation did not increase extracted amidase activity. However, changes in Western blot analysis profiles were visible in sperm extract and seminal plasma after cryopreservation. After freezing-thawing, additional bands appeared in sperm extract and seminal plasma. These bands were of different molecular weight regarding the presence or absence of NPGB. These data suggest that the mechanism of damage to the proacrosin/acrosin system is different for liquid storage and cryopreservation. Liquid storage seems to increase in the susceptibility of the proacrosin/acrosin system to be activated during extraction. Kazal inhibitors of turkey seminal plasma are involved in the control of proacrosin activation. The disturbances of the proacrosin/acrosin system of turkey spermatozoa can be related to a disturbance in the induction of the acrosome reaction. Our results may be important for a better understanding of the proacrosin/acrosin system of turkey spermatozoa and disturbance to this system during liquid storage and cryopreservation. Copyright © 2012 Elsevier Inc. All rights reserved.

  19. Characterization of vacuum-packed and irradiated frozen turkey meat (Meleagris gallopavo)

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    Henry, Fabio C.; Valle, Felipe R.F.A. do; Moulin, Carlos H.S., E-mail: fabiocosta@uenf.b [Universidade Estadual do Norte Fluminense (UENF), Campos dos Goytacazes, RJ (Brazil). Centro de Ciencias e Tecnologias Agropecuarias; Silva, Teofilo J.P.; Franco, Robson M.; Freitas, Monica Q., E-mail: mtatjps@vm.uff.b [Universidade Federal Fluminense (UFF), Niteroi, RJ (Brazil). Fac. de Medicina Veterinaria. Dept. de Tecnologia de Alimentos; Vital, Helio C., E-mail: vital@ctex.eb.b [Centro Tecnologico do Exercito (CTEx), Rio de Janeiro, RJ (Brazil). Div. de Defesa Quimica, Biologica e Nuclear. Secao de Defesa Nuclear; Jesus, Edgar F.O. de, E-mail: edgar@lin.ufrj.b [Universidade Federal do Rio de Janeiro (UFRJ), RJ (Brazil). Coordenacao dos Programas de Pos-Graduacao em Engenharia

    2011-07-01

    Irradiation is known to enhance the sanitary quality and extend the commercial shelf life of many kinds of food such as meat products. In this work, boned breasts from nine male turkeys, slaughtered according to the surveillance requirements of the Brazilian Federal Inspection Service, were purchased from a registered producer. They were then cut, vacuum packed, frozen at -18 degree C, exposed to gamma radiation at doses of 1 and 3kGy and kept in storage at -18 degree C for up to 540 days. Chemical analyses as well as sensory tests for taste, color and overall impression were performed on days 5, 180, 360 and 540 of storage. Statistical analyses were performed in order to investigate possible significant effects arising from the combination of treatments used (a- freezing, b- freezing and irradiation with 1kGy and c- freezing and irradiation with 3kGy) as functions of time. In the beginning of storage, the levels of lipids in samples irradiated with 3 kGy were about twice those found in unirradiated ones, with TBARS values increasing with storage time in all samples. However, the results from the sensory tests performed have indicated that irradiation with doses of 1 and 3 kGy does not significantly impact the acceptance of taste, flavor, color or the overall sensory impression of frozen turkey breast meat. (author)

  20. Characterization of vacuum-packed and irradiated frozen turkey meat (Meleagris gallopavo)

    International Nuclear Information System (INIS)

    Henry, Fabio C.; Valle, Felipe R.F.A. do; Moulin, Carlos H.S.; Silva, Teofilo J.P.; Franco, Robson M.; Freitas, Monica Q.; Vital, Helio C.; Jesus, Edgar F.O. de

    2011-01-01

    Irradiation is known to enhance the sanitary quality and extend the commercial shelf life of many kinds of food such as meat products. In this work, boned breasts from nine male turkeys, slaughtered according to the surveillance requirements of the Brazilian Federal Inspection Service, were purchased from a registered producer. They were then cut, vacuum packed, frozen at -18 degree C, exposed to gamma radiation at doses of 1 and 3kGy and kept in storage at -18 degree C for up to 540 days. Chemical analyses as well as sensory tests for taste, color and overall impression were performed on days 5, 180, 360 and 540 of storage. Statistical analyses were performed in order to investigate possible significant effects arising from the combination of treatments used (a- freezing, b- freezing and irradiation with 1kGy and c- freezing and irradiation with 3kGy) as functions of time. In the beginning of storage, the levels of lipids in samples irradiated with 3 kGy were about twice those found in unirradiated ones, with TBARS values increasing with storage time in all samples. However, the results from the sensory tests performed have indicated that irradiation with doses of 1 and 3 kGy does not significantly impact the acceptance of taste, flavor, color or the overall sensory impression of frozen turkey breast meat. (author)

  1. Macro and Microanatomical Studies on the Choanal Slit of Turkey (Meleagris gallopavo

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    Ramy K. A. Sayed

    2017-07-01

    Full Text Available This work was carried out to describe the morphological characteristics of the choanal slit of the turkey through gross, light, and scanning electron microscopy. The choanal slit measures 27.62 mm long, and constitutes 38.30 % of the total length of the palate. The edges of the narrow part of the choanal slit is smooth rostrally but slightly thickened caudally due to the presence of 2-3 small papillae. The edge of the wide part is thickened because of presence of 5-7 conical and wedge shaped papillae. SEM indicates the presence of median fold within the choana, which represents the direct continuation of the median palatine ridge. After a short distance, this fold bifurcates into right and left folds. Several openings of the palatine salivary glands are demonstrated on the palate at the level of the choanal slit. The epithelium of the oral roof at the level of the choanal slit is stratified squamous epithelium showing intraepithelial sensory corpuscles. This epithelium transforms at the edge of the choanal slit into pseudostratified ciliated columnar epithelium that interrupted by intraepithelial mucous glands surrounded by lymphatic infiltration and nodules. Altogether, this study provides inclusive information on the macroscopic and microscopic morphological features of the choana in the turkey in comparing with those of the other birds.

  2. Review of the social and environmental factors affecting the behavior and welfare of turkeys (Meleagris gallopavo).

    Science.gov (United States)

    Marchewka, J; Watanabe, T T N; Ferrante, V; Estevez, I

    2013-06-01

    In modern rearing systems, turkey producers often face economic losses due to increased aggression, feather pecking, cannibalism, leg disorders, or injuries among birds, which are also significant welfare issues. The main underlying causes appear to relate to rapid growth, flock size, density, poor environmental complexity, or lighting, which may be deficient in providing the birds with an adequate physical or social environment. To date, there is little information regarding the effect of these factors on turkey welfare. This knowledge is, however, essential to ensure the welfare of turkeys and to improve their quality of life, but may also be beneficial to industry, allowing better bird performance, improved carcass quality, and reduced mortality and condemnations. This paper reviews the available scientific literature related to the behavior of turkeys as influenced by the physical and social environment that may be relevant to advances toward turkey production systems that take welfare into consideration. We addressed the effects that factors such as density, group size, space availability, maturation, lightning, feeding, and transport may have over parameters that may be relevant to ensure welfare of turkeys. Available scientific studies were based in experimental environments and identified individual factors corresponding to particular welfare problems. Most of the studies aimed at finding optimal levels of rearing conditions that allow avoiding or decreasing most severe welfare issues. This paper discusses the importance of these factors for development of production environments that would be better suited from a welfare and economic point of view.

  3. Microsatellite marker-based genetic analysis of relatedness between commercial and heritage turkeys (Meleagris gallopavo).

    Science.gov (United States)

    Kamara, D; Gyenai, K B; Geng, T; Hammade, H; Smith, E J

    2007-01-01

    The turkey is second only to the chicken in importance as an agriculturally important poultry species. Unlike the chicken, however, genetic studies of the turkey continue to be limited. For example, to date, many genomic investigations have been conducted to characterize genetic relationships between commercial (CO) and non-CO chicken breeds, whereas the nature of the genetic relatedness between CO and heritage turkeys remains unknown. The objective of the current research was to use microsatellites to analyze the genetic relatedness between CO and heritage domestic turkeys including Narragansett, Bourbon Red, Blue Slate, Spanish Black, and Royal Palm. Primer pairs specific for 10 previously described turkey microsatellite markers were used. The phylogenetic analysis showed that the Blue Slate, Bourbon Red, and Narragansett were genetically closely related to the CO strain, with a Nei distance of 0.30, and the Royal Palm and Spanish Black were the least related to the CO strain, with Nei distances of 0.41 and 0.40, respectively. The present work provides a foundation for the basis of using heritage turkeys to genetically improve CO populations by introgression.

  4. UV reflection properties of plumage and skin of domesticated turkeys (Meleagris gallopavo f. dom.) as revealed by UV photography.

    Science.gov (United States)

    Bartels, T; Lütgeharm, J-H; Wähner, M; Berk, J

    2017-12-01

    Reflection and fluorescence properties of feathered and non-feathered body regions of white- and bronze-colored fattening turkeys of various ages were examined by ultraviolet (UV) photography. The examinations were carried out on 20 white-feathered fattening turkeys (B.U.T. 6; 10 males, 10 females) and 20 bronze-feathered fattening turkeys (Grelier 708; 10 males, 10 females) over a period of 21 weeks. The turkeys were photographed once a wk under long-wave UV (UVA) radiation illumination (λ = 344-407 nm) using a digital camera. A bandpass filter was used for UV reflectography to filter out the visible components of the used light source. A longpass filter was used for UV fluorescence photography to avoid blurring in the image due to chromatic aberration as a result of UV illumination. We found that natal down feathers of white-feathered turkeys showed an intense yellowish-green fluorescence under UVA light. UVA fluorescence also was shown by the natal downs of the slightly melanized plumage areas of bronze turkeys. Vaned feathers of white fattening turkeys reflected UVA radiation. Freshly molted feathers were optically distinguishable from the previous feather generation due to their more intense UVA reflection. In bronze turkeys, both the bright end seams of the dark pennaceous feathers and rectrices and the bright banding of primary and secondary remiges reflected UVA radiation. Intense UVA fluorescence was recognizable in day-old chicks of both color variants on the scutellate scales of the legs and toes. In male turkeys of both color variants, UVA-reflecting parts were recognizable with increasing age on the featherless head region. The UVA-fluorescent and UVA-reflective characteristics of the plumage of fattening turkeys were closely related to the plumage color, the feather type, the molting state, and the age of the birds. Further research is needed regarding the UVA-reflecting properties of the turkey plumage and the effects of full-spectrum illumination, including the UVA spectrum, on the behavior and health of fattening turkeys. © 2017 Poultry Science Association Inc.

  5. Feasibility study on the FAO chicken microsatellite panel to assess genetic variability in the turkey (Meleagris gallopavo

    Directory of Open Access Journals (Sweden)

    Elena Colombo

    2014-12-01

    Full Text Available The aim of this work was to study the feasibility of the Food and Agriculture Organization (FAO microsatellite panel developed for chickens to assess genetic variability in turkeys. Genomic DNA was extracted from a total of 37 blood samples collected from turkey of different breeds [15 Brianzolo (BR; 12 Colli Euganei (EU; 10 Nero d’Italia (NI], and all 31 chicken microsatellite markers recommended by the FAO were tested. The results show that 22 chicken markers out of 31 suggested by FAO guidelines can be applied to turkey populations. In particular, the multiplex groups confirmed in the turkey were the Multiplex Master Mix 1 (ADL0268, ADL0278, LEI0094, MCW0216, MCW0248 and the Master Mix 2 (MCW0034, MCW0069, MCW0081, MCW0222, MCW0295, whereas 13 microsatellites were amplified only under single polymerase chain reaction (PCR conditions. No PCR products were obtained for 9 markers (LEI0166, MCW0020, MCW0078, MCW0080, MCW0104, MCW0123, MCW0248, MCW0284 and MCW0330, which is 29% of the total markers used. A panel of 22 markers was used to assess genetic diversity in three turkey breeds and a total number of 63 alleles were found. Observed (Ho and expected (He heterozygosity and polymorphism information content (PIC values for each microsatellite and the relative mean values were also calculated. The mean values were 0.210, 0.250, 0.203 for Ho; 0.301, 0.348, 0.228 for He; and 0.265, 0.313, 0.199 for PIC in NI, BR and EU, respectively.

  6. Microscopic morphology and apoptosis of ovarian tissue after cryopreservation using a vitrification method in post-hatching turkey poults, Meleagris gallopavo

    Science.gov (United States)

    1. Microscopic morphology of ovarian tissue in post-hatching turkey poults at various ages was investigated. 2. Hematoxylin and eosin staining were used and the diameter of the oocytes and follicles were measured using microphotography. 3. Immediately after hatching, oocytes in one-day turkey pou...

  7. Fecundidad de la medusa Stomolophus meleagris (Rhizostomeae: Stomolophidae en el Golfo de California Stomolophus meleagris fecundity (Rhizostomeae: Stomolophidae in the Gulf of California

    Directory of Open Access Journals (Sweden)

    Liliana Carvalho Saucedo

    2012-12-01

    Full Text Available La medusa Stomolophus meleagris es considerada un recurso pesquero de alto valor comercial. Debido a la relevancia de conocer aspectos de importancia biológica actualmente desconocidos, el presente trabajo analiza la fecundidad con base en la estimación del número de ovocitos vitelogénicos en la gónada; su relación con la longitud, diámetro y peso húmedo; y la relación del índice gonadosomático (IGS con su diámetro y longitud. De enero a mayo 2006 se recolectaron 30 ejemplares mensuales que fueron medidos y pesados, de los cuales se analizaron 60 hembras maduras para calcular el volumen de sus gónadas. Se extrajeron las gónadas, se tiñeron con hematoxilina-eosina y se seleccionaron las hembras con madurez gonadal. Mediante la digitalización de imágenes se estimó el número de ovocitos vitelogénicos y previtelogénicos. Se emplearon datos de captura en Las Guásimas para el análisis de reclutamiento poblacional, estimado con FISAT II. La fecundidad se incrementó con la longitud, el diámetro y el peso húmedo total. La menor fecundidad se observó en febrero y la mayor en mayo (11 873 071 y 37 528 197 millones de ovocitos vitelogénico por gónada, respectivamente. El IGS se incrementó con la longitud y diámetro de los ejemplares, con su máximo en mayo de 3.7%. Se observó una producción continua de ovocitos previtelogénicos en todo el periodo de recolecta. El máximo reclutamiento se observó en julio y noviembre (37.50% y 28.01% respectivamente. La alta fecundidad encontrada en S. meleagris sugiere un alto potencial reproductivo para la población y sustenta la posibilidad de la consolidación de una importante pesquería de esta medusa.The cannonball jellyfish Stomolophus meleagris is regarded as a fishery resource with high commercial value, but with scarce biological information. With the aim to generate preliminary information on reproductive aspects, the present study analyzes its fecundity, based on the estimated

  8. Further Insights into the Catalytical Properties of Deglycosylated Pyranose Dehydrogenase from Agaricus meleagris Recombinantly Expressed in Pichia pastoris

    OpenAIRE

    Yakovleva, Maria E.; Killyeni, Aniko; Seubert, Oliver; Conghaile, Peter O.; MacAodha, Domhnall; Leech, Donal; Gonaus, Christoph; Popescu, Ionel Catalin; Peterbauer, Clemens K.; Kjellstrom, Sven; Gorton, Lo

    2013-01-01

    The present study focuses on fragmented deglycosylated pyranose dehydrogenase (fdgPDH) from Agaricus meleagris recombinantly expressed in Pichia pastoris. Fragmented deglycosylated PDH is formed from the deglycosylated enzyme (dgPDH) when it spontaneously loses a C-terminal fragment when stored in a buffer solution at 4 °C. The remaining larger fragment has a molecular weight of ∼46 kDa and exhibits higher volumetric activity for glucose oxidation compared with the deglycosylated and glycosyl...

  9. Genome-wide candidate regions for selective sweeps revealed through massive parallel sequencing of DNA across ten turkey populations

    NARCIS (Netherlands)

    Aslam, M.L.; Bastiaansen, J.W.M.; Megens, H.J.W.C.; Crooijmans, R.P.M.A.; Blomberg, L.; Groenen, M.

    2014-01-01

    Background The domestic turkey (Meleagris gallopavo) is an important agricultural species that is largely used as a meat-type bird. Characterizing genetic variation in populations of domesticated species and associating these variation patterns with the evolution, domestication, and selective

  10. Influência do dimorfismo sexual sobre a morfologia da siringe de galinha d'angola (Numida meleagris

    Directory of Open Access Journals (Sweden)

    Bottino Flávia

    2006-01-01

    Full Text Available Este estudo teve como objetivo realizar uma descrição morfológica e comparativa da siringe, órgão responsável pelo canto das aves, na espécie Numida meleagris. Para isso foram utilizados cinco machos e cinco fêmeas de galinha d'angola, a fim de verificar a sintopia (traquéia, músculos traqueais e o dimorfismo sexual da siringe. Verificou-se que a siringe se localiza na bifurcação da traquéia e apresenta maior número de cartilagens nos machos. Nos machos, a inserção do músculo traqueal lateral bem como a origem do músculo esterno traqueal localizam-se mais caudalmente e são mais largos em relação às fêmeas. As diferenças existentes entre machos e fêmeas de galinha d'angola revelam a elevada capacidade das fêmeas em produzir sons semelhantes a "tô fraco" enquanto que os machos emitem arrulhos e cacarejos.

  11. Sperm subpopulations in avian species: a comparative study between the rooster (Gallus domesticus) and Guinea fowl (Numida meleagris).

    Science.gov (United States)

    García-Herreros, Manuel

    2016-01-01

    The main aims of this research were to study possible differences in objective morphometric sperm characteristics, establish normative sperm morphometry standards, and evaluate the presumed different subpopulation distribution of avian spermatozoa from the rooster (Gallus domesticus ) and Guinea fowl (Numida meleagris ) as model avian species. Seventy-two ejaculates (36 per species studied) were obtained manually, following a training period involving gently combined dorso-abdominal and lumbo-sacral massage of the birds. Ejaculates were processed for volume, sperm concentration, viability, motility, and morphology. Moreover, samples were submitted for sperm morphometric assessment using objective Computer-Assisted Semen Analysis for Morphometry (CASA-Morph) methods, with sperm morphometric descriptors evaluated by Principal Component Analysis (PCA) and multivariate clustering analyses. There were several differences observed between the avian species in values obtained for ejaculate volume and sperm concentration (P rooster and Guinea fowl, respectively). Moreover, the distribution of the sperm subpopulations was found to be structurally different between species. In conclusion, our findings from using CASA-Morph methods indicate pronounced sperm morphometric variation between these two avian species. Because of the strong differences observed in morphometric parameter values and their subpopulation distribution, these results suggest that application of objective analytical methods such as CASA-Morph could substantially improve the reliability of comparative studies and help establish valid normative sperm morphological values for avian species.

  12. Sperm subpopulations in avian species: a comparative study between the rooster (Gallus domesticus and Guinea fowl (Numida meleagris

    Directory of Open Access Journals (Sweden)

    Manuel García-Herreros

    2016-01-01

    Full Text Available The main aims of this research were to study possible differences in objective morphometric sperm characteristics, establish normative sperm morphometry standards, and evaluate the presumed different subpopulation distribution of avian spermatozoa from the rooster (Gallus domesticus and Guinea fowl (Numida meleagris as model avian species. Seventy-two ejaculates (36 per species studied were obtained manually, following a training period involving gently combined dorso-abdominal and lumbo-sacral massage of the birds. Ejaculates were processed for volume, sperm concentration, viability, motility, and morphology. Moreover, samples were submitted for sperm morphometric assessment using objective Computer-Assisted Semen Analysis for Morphometry (CASA-Morph methods, with sperm morphometric descriptors evaluated by Principal Component Analysis (PCA and multivariate clustering analyses. There were several differences observed between the avian species in values obtained for ejaculate volume and sperm concentration (P < 0.001. Irrespective of species, PCA revealed two Principal Components (PCs explaining more than 80% of the variance. In addition, the number of subpopulations differed with species (three and five subpopulations for rooster and Guinea fowl, respectively. Moreover, the distribution of the sperm subpopulations was found to be structurally different between species. In conclusion, our findings from using CASA-Morph methods indicate pronounced sperm morphometric variation between these two avian species. Because of the strong differences observed in morphometric parameter values and their subpopulation distribution, these results suggest that application of objective analytical methods such as CASA-Morph could substantially improve the reliability of comparative studies and help establish valid normative sperm morphological values for avian species.

  13. Investigation of the effect of tribulus terrestris extract on the main biochemical and haematological indices of the blood in guinea fowls (Numida meleagris

    Directory of Open Access Journals (Sweden)

    Christo CHRISTEV

    2011-07-01

    Full Text Available The aim of the present research was to investigate the effect of Bulgarian additive Vemoherb-T (dry extract of the annual plant Tribulus terrestris – L, produced by Vemo 99 Ltd Company, Sofia on main biochemical characteristics and hematological parameters of the blood in guinea fowl (Numida meleagris. An experiment was carried out with 30 Pearl-gray Guinea fowl (32 weeks old, distributed in two groups – a control and an experimental, 12 female and 3 male each. All birds were fed the same mixture for breeder guinea fowl. Vemoherb-T was supplemented to the compound feed of the experimental group in a daily dose of 10 mg/kg body weight for a period of 12 weeks. The tested product decreased significantly the levels of total triglycerides (P < 0.05, total cholesterol (P <0.01 and glucose (P < 0.01; P < 0.001 in male and female birds respectively in the blood serum. It was established significantly higher total protein- ((P < 0.001 and calcium (P<0.01 values in the blood serum of the treated birds. The addition of Vemoherb-T increased significantly hemoglobin level, the number of erythrocytes and leukocytes and decreased the number of eosinophils in guinea fowl from the both sexes.

  14. The 1.6 Å crystal structure of pyranose dehydrogenase from Agaricus meleagris rationalizes substrate specificity and reveals a flavin intermediate.

    Directory of Open Access Journals (Sweden)

    Tien Chye Tan

    Full Text Available Pyranose dehydrogenases (PDHs are extracellular flavin-dependent oxidoreductases secreted by litter-decomposing fungi with a role in natural recycling of plant matter. All major monosaccharides in lignocellulose are oxidized by PDH at comparable yields and efficiencies. Oxidation takes place as single-oxidation or sequential double-oxidation reactions of the carbohydrates, resulting in sugar derivatives oxidized primarily at C2, C3 or C2/3 with the concomitant reduction of the flavin. A suitable electron acceptor then reoxidizes the reduced flavin. Whereas oxygen is a poor electron acceptor for PDH, several alternative acceptors, e.g., quinone compounds, naturally present during lignocellulose degradation, can be used. We have determined the 1.6-Å crystal structure of PDH from Agaricus meleagris. Interestingly, the flavin ring in PDH is modified by a covalent mono- or di-atomic species at the C(4a position. Under normal conditions, PDH is not oxidized by oxygen; however, the related enzyme pyranose 2-oxidase (P2O activates oxygen by a mechanism that proceeds via a covalent flavin C(4a-hydroperoxide intermediate. Although the flavin C(4a adduct is common in monooxygenases, it is unusual for flavoprotein oxidases, and it has been proposed that formation of the intermediate would be unfavorable in these oxidases. Thus, the flavin adduct in PDH not only shows that the adduct can be favorably accommodated in the active site, but also provides important details regarding the structural, spatial and physicochemical requirements for formation of this flavin intermediate in related oxidases. Extensive in silico modeling of carbohydrates in the PDH active site allowed us to rationalize the previously reported patterns of substrate specificity and regioselectivity. To evaluate the regioselectivity of D-glucose oxidation, reduction experiments were performed using fluorinated glucose. PDH was rapidly reduced by 3-fluorinated glucose, which has the C2

  15. Influence of mountain pine beetle epidemic on winter habitat conditions for Merriam's turkeys: Management implications for current and future condition

    Science.gov (United States)

    Chadwick P. Lehman; Mark A. Rumble; Michael A. Battaglia; Todd R. Mills; Lance A. Asherin

    2016-01-01

    Understanding response of ponderosa pine (Pinus ponderosa) forest development following a mountain pine beetle (MPB; Dendroctonus ponderosae) epidemic has important management implications for winter habitat conditions for Merriam’s wild turkeys (Meleagris gallopavo merriami; hereafter, turkeys). Therefore, we quantified habitat changes over time for turkeys...

  16. Multiple-scale roost habitat comparisons of female Merriam's wild turkeys in the southern Black Hills, South Dakota

    Science.gov (United States)

    Daniel J. Thompson; Mark A. Rumble; Lester D. Flake; Chad P. Lehman

    2009-01-01

    Because quantity and quality of roosting habitat can affect Merriam's Wild Turkey (Meleagris gallopavo merriami) distribution, we described habitat characteristics of Merriam's turkey roost sites in the southern Black Hills of South Dakota. Varying proportions of Merriam's turkeys in the southern Black Hills depended on supplemental feed from livestock...

  17. Roosting habitat of Merriam's turkeys in the Black Hills, South Dakota

    Science.gov (United States)

    Mark A. Rumble

    1992-01-01

    Lack of roost habitat (trees >40 cm diameter breast height [dbh] and >18 m2/ha basal area) can limit populations of Merriam’s turkeys (Meleagris gallopavo merriami). The Black Hills region has relatively large populations of Merriam’s turkeys, yet trees >40 cm dbh are uncommon. Consequently, I studied...

  18. Resource selection for foraging by female Merriam's wild turkeys with poults in the southern Black Hills, South Dakota

    Science.gov (United States)

    Chad P. Lehman; Mark A. Rumble; Lester D. Flake; Daniel J. Thompson

    2011-01-01

    Knowledge of Merriam's wild turkey (Meleagris gallopavo merriami) resource selection in the context of landscape attributes is an important asset for managing resources on multiple-use public lands. We investigated resource selection for foraging by Merriam's wild turkey broods in the southern Black Hills, South Dakota. We collected macro- and microhabitat...

  19. Effects of two commercial neem-based insecticides on lone star tick, Amblyomma americanum (L.) (Acari: Ixodidae): deterrence, mortality, and reproduction

    Science.gov (United States)

    The lone star tick, Amblyomma americanum (L.), is a widely distributed three-host obligate blood-feeding parasite in the United States and Mexico. It mostly attaches to white-tailed deer, Odocoilus virginianus (Zimmerman) and wild turkey, Meleagris gallopavo L., as well as a wide variety of other do...

  20. Polymorphic microsatellites developed by cross-species amplifications in common pheasant breeds

    NARCIS (Netherlands)

    Baratti, M.; Alberti, A.; Groenen, M.A.M.; Veenendaal, T.; Fulgheri, F.D.

    2001-01-01

    Genetic variability was analysed in two common breeds of pheasant (Phasianus colchicus L. 1758) by means of cross-species amplifications of microsatellite loci: 154 chicken, Gallus gallus and 32 turkey, Meleagris gallopavo, primers were tested for amplification of pheasant DNA. Thirty-six primers

  1. How far could a squirrel travel in the treetops? A prehistory of the southern forest

    Science.gov (United States)

    Paul B. Hamel; Edward R. Buckner

    1998-01-01

    Conservation activities aimed at protecting old-growth forests; at maintaining populations of desired species groups, such as oaks (Quercus sp.), wild turkeys (Meleagris gallopavo), other game species or Neotropical migratory birds; and at increasing populations of endangered species, such as red-cockaded woodpeckers (Picoides borealis), Bachman's warblers (...

  2. African Journal of Biotechnology - Vol 10, No 68 (2011)

    African Journals Online (AJOL)

    Alternative substrates for production of Heliconia psittacorum L. seedlings under shade and open field conditions · EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT ... Effect of turkey litter (Meleagris gallopavo L.) vermicompost on growth and yield characteristics of paddy, Oryza sativa (ADT-37) · EMAIL FREE FULL TEXT ...

  3. Finding of No Significant Impact & Tiered Environmental Assessment: Public Law 84-99 Rehabilitation Program Levee Unit 624-627 - Mosquito Creek Pottawattamie County, Iowa

    Science.gov (United States)

    2014-10-01

    spp.), northern flicker (Colaptes auratus), red-headed woodpecker (Melanerpes erythrocephalus), wild turkey (Meleagris gallopavo), eastern kingbird...aquatic environment. Some commenters asked that compensatory mitigation be required for all activities authorized by this NWP. A few commenters...remarked that compensatory mitigation should be required for adverse effects on high quality riparian areas. Another commenter said that mitigation should

  4. A test of the habitat suitability model for Merriam's wild turkeys

    Science.gov (United States)

    Mark A. Rumble; Stanley H. Anderson

    1996-01-01

    An important research area regarding the wild turkey (Meleagris gallopavo) is development of sound habitat models. Habitat models provide standardized methods to quantify wild turkey habitat and stimulate new research hypotheses. Habitat suitability index (HSI) models show species-habitat relationships on a scale of O-l, with 1 being optimum. A...

  5. Macrohabitat associations of Merriam's turkeys in the Black Hills, South Dakota

    Science.gov (United States)

    Mark A. Rumble; Stanley H. Anderson

    1993-01-01

    Merriam's turkeys (Meleagris gallopavo merriami) were introduced into South Dakota in the late 1940's and have since expanded to occupy the entire Black Hills. Because little is known of their habitat requirements and the effects of forest management practices on this important game species, macrohabitat selections patterns of Merriam'...

  6. Microhabitats of Merriam's turkeys in the Black Hills, South Dakota

    Science.gov (United States)

    Mark A. Rumble; Stanley H. Anderson

    1996-01-01

    Merriam’s Turkeys (Meleagris gallopavo merriami) are associated with ponderosa pine (Pinus ponderosa) forests in the western United States, but are not native to the ponderosa pine forest of the Black Hills, South Dakota. The Black Hills population was established by transplanting birds from New Mexico and Colorado between 1948 and...

  7. Variation in selection of microhabitats by Merriam's turkey brood hens

    Science.gov (United States)

    Mark A. Rumble; Stanley H. Anderson

    1997-01-01

    We studied microhabitats of Merriam‘s turkey (Meleagris gallopavo merriami) brood hens in a ponderosa pine (Pinus ponderosa) ecosystem in South Dakota from 1986 to 1988. Cluster analysis indicated three groups of microhabitats, open-shrub, open-grasslforb and forest, based on vegetation characteristics at sites selected by brood...

  8. Evaluating the microscopic fecal technique for estimating hard mast in turkey diets

    Science.gov (United States)

    Mark A. Rumble; Stanley H. Anderson

    1993-01-01

    Wild and domestic dark turkeys (Meleagris gallopavo) were fed experimental diets containing acorn (Quercus gambelli), ponderosa pine (Pinus ponderosa) seed, grasses, forbs, and arthropods. In fecal estimates of diet composition, acorn and ponderosa pine seed were underestimated and grass was overestimated....

  9. Genetic control and variation in turkey: molecular insights in selection

    NARCIS (Netherlands)

    Aslam, M.L.

    2012-01-01


    The turkey (Meleagris gallopavo) is an important agricultural species that is largely used as a meat type bird as egg production of this species is very low. Turkey is the second largest contributor to the world’s poultry meat production after chicken. Understanding the etiology and

  10. Variación lipídica en los ovocitos de la medusa Stomolophus meleagris (Scyphozoa: Rhizostomeae, durante el desarrollo gonádico, en la laguna Las Guásimas, Sonora, México

    Directory of Open Access Journals (Sweden)

    Liliana Carvalho-Saucedo

    2010-03-01

    Full Text Available La medusa S. meleagris, ha mostrado potencial de explotación pero hay escasa información sobre su biología reproductiva. El presente trabajo pretende conocer el contenido de los triglicéridos y fosfolípidos en los ovocitos durante el desarrollo gonadal, así como la proporción de sexos, talla de primera madurez y la concentración de proteínas y lípidos totales en la medusa. Se realizaron muestreos mensuales durante 2005 y 2006. A las medusas recolectadas en 2005, se les aplicó la técnica del sudán negro, para describir las características del ovocito y la cantidad triglicéridos y fosfolípidos y la técnica de hematoxilina-eosina para conocer el grado de desarrollo gonádico y la proporción de sexos. Las medusas del 2006 se emplearon para determinar la talla de primera madurez y el contenido de proteínas y lípidos totales. Se observaron cuatro fases de desarrollo en ambos sexos, con un desarrollo gamético continúo. El mayor porcentaje de organismos maduros se registró en abril. La proporción de sexos fue de 0.7:1.3. Se encontró mayor concentración de triglicéridos que de fosfolípidos en el citoplasma. Se obtuvo una correlación positiva entre triglicéridos y el diámetro del ovocito. La talla de primera madurez para ambos sexos fue de 105 mm. El mayor contenido de proteínas se obtuvo en abril y para lípidos en marzo.Lipid variation in oocytes of the jellyfish Stomolophus meleagris (Scyphozoa: Rhizostomeae from Las Guasimas Lagoon, Mexico, during gonadal development. The jellyfish Stomolophus meleagris has potential for commercial exploitation but there is little information on their reproductive biology. This paper seeks to evaluate some biochemical and demographic characteristics of the species. Samples were taken monthly during 2005 and 2006. Jellyfish collected in 2005 were used to describe the characteristics and quantity of oocyte triglycerides and phospholipids with the Sudan black technique, and to ascertain

  11. Superoxide activates a GDP-sensitive proton conductance in skeletal muscle mitochondria from king penguin (Aptenodytes patagonicus).

    Science.gov (United States)

    Talbot, Darren A; Hanuise, Nicolas; Rey, Benjamin; Rouanet, Jean-Louis; Duchamp, Claude; Brand, Martin D

    2003-12-26

    We present the partial nucleotide sequence of the avian uncoupling protein (avUCP) gene from king penguin (Aptenodytes patagonicus), showing that the protein is 88-92% identical to chicken (Gallus gallus), turkey (Meleagris gallopavo), and hummingbird (Eupetomena macroura). We show that superoxide activates the proton conductance of mitochondria isolated from king penguin skeletal muscle. GDP abolishes the superoxide-activated proton conductance, indicating that it is mediated via avUCP. In the absence of superoxide there is no GDP-sensitive component of the proton conductance from penguin muscle mitochondria demonstrating that avUCP plays no role in the basal proton leak.

  12. Mycoplasma gallopavonis in eastern wild turkeys.

    Science.gov (United States)

    Luttrell, M P; Eleazer, T H; Kleven, S H

    1992-04-01

    Serum samples and tracheal cultures were collected from eastern wild turkeys (Meleagris gallopavo sylvestris) trapped for relocation in South Carolina (USA) during 1985 to 1990. Sera were tested for Mycoplasma gallisepticum and M. synoviae by the rapid plate agglutination and hemagglutination inhibition tests and were found to be negative. Tracheal cultures were negative for all pathogenic Mycoplasma spp., including M. gallisepticum, M. synoviae, M. meleagridis, and M. iowae. However, M. gallopavonis was isolated from every group of wild turkeys tested in 1986 to 1990. These data suggest that M. gallopavonis, which is generally considered nonpathogenic, may be a common microorganism in eastern wild turkeys.

  13. Post-brooding management of local guinea fowls ( Numida meleagris )

    African Journals Online (AJOL)

    Amposem, I Adam. Abstract. No abstract. Bulletin of Animal Health and Production in Africa Vol. 54 (4) 2006: pp. 270-276. Full Text: EMAIL FULL TEXT EMAIL FULL TEXT · DOWNLOAD FULL TEXT DOWNLOAD FULL TEXT.

  14. Plasmodium durae Herman from the introduced common peafowl in northern Nigeria.

    Science.gov (United States)

    Laird, M

    1978-02-01

    Plasmodium (Giovannolaia) durae Herman was originally described from Kenya, the type host being the common turkey, Meleagris gallopavo Linnaeus. There are no field records of this association outside of Africa, where the parasite, herein reported from another introduced and domesticated bird (the common peafowl, Pavo cristatus Linnaeus), was recently listed from 2 native Phasianidae of the genus Francolinus. The justification for the present identification is submitted against background data concerning malaria parasites from turkeys and other Galliformes in Africa and elsewhere, and restraint is urged in describing yet more "new species" of avian Plasmodium belonging to morphologically close taxa within Novyella and Giovannolaia. A near relative of P. durae, Plasmodium dissanaikei de Jong, is transferred from the former subgenus to the latter one.

  15. The complete mitochondrial genome sequence of Eimeria innocua (Eimeriidae, Coccidia, Apicomplexa).

    Science.gov (United States)

    Hafeez, Mian Abdul; Vrba, Vladimir; Barta, John Robert

    2016-07-01

    The complete mitochondrial genome of Eimeria innocua KR strain (Eimeriidae, Coccidia, Apicomplexa) was sequenced. This coccidium infects turkeys (Meleagris gallopavo), Bobwhite quails (Colinus virginianus), and Grey partridges (Perdix perdix). Genome organization and gene contents were comparable with other Eimeria spp. infecting galliform birds. The circular-mapping mt genome of E. innocua is 6247 bp in length with three protein-coding genes (cox1, cox3, and cytb), 19 gene fragments encoding large subunit (LSU) rRNA and 14 gene fragments encoding small subunit (SSU) rRNA. Like other Apicomplexa, no tRNA was encoded. The mitochondrial genome of E. innocua confirms its close phylogenetic affinities to Eimeria dispersa.

  16. Isolation of Mycoplasma gallopavonis from free-ranging wild turkeys in coastal North Carolina seropositive and culture-negative for Mycoplasma gallisepticum.

    Science.gov (United States)

    Cobb, D T; Ley, D H; Doerr, P D

    1992-01-01

    Serum samples and choanal cleft swabs were collected from livetrapped and hunter killed wild turkeys (Meleagris gallopavo) from Martin and Bertie counties, North Carolina (USA). Sera were tested for antibodies to Mycoplasma gallisepticum, Mycoplasma synoviae and Mycoplasma meleagridis by hemagglutination inhibition (HI). Sera from 33% (five of 15) of livetrapped turkeys were positive for antibodies to M. gallisepticum by HI, and all were negative for antibodies to M. synoviae and M. meleagridis. Choanal cleft swabs from 22 livertrapped and five hunter killed wild turkeys cultured in Frey's broth medium resulted in 23 mycoplasma isolations. Using direct immunofluorescence, 74% (17/23) were M. gallopavonis, and 26% (six of 23) were unidentified; no isolate was identified as M. gallisepticum, M. synoviae or M. meleagridis.

  17. Species identification in meat products: A new screening method based on high resolution melting analysis of cyt b gene.

    Science.gov (United States)

    Lopez-Oceja, A; Nuñez, C; Baeta, M; Gamarra, D; de Pancorbo, M M

    2017-12-15

    Meat adulteration by substitution with lower value products and/or mislabeling involves economic, health, quality and socio-religious issues. Therefore, identification and traceability of meat species has become an important subject to detect possible fraudulent practices. In the present study the development of a high resolution melt (HRM) screening method for the identification of eight common meat species is reported. Samples from Bos taurus, Ovis aries, Sus scrofa domestica, Equus caballus, Oryctolagus cuniculus, Gallus gallus domesticus, Meleagris gallopavo and Coturnix coturnix were analyzed through the amplification of a 148 bp fragment from the cyt b gene with a universal primer pair in HRM analyses. Melting profiles from each species, as well as from several DNA mixtures of these species and blind samples, allowed a successful species differentiation. The results demonstrated that the HRM method here proposed is a fast, reliable, and low-cost screening technique. Copyright © 2017 Elsevier Ltd. All rights reserved.

  18. Diversity of management strategies in Mesoamerican turkeys: archaeological, isotopic and genetic evidence

    Science.gov (United States)

    Manin, Aurelie; Corona-M, Eduardo; Craig, Abigail; Thornton, Erin Kennedy; Yang, Dongya Y.; Richards, Michael

    2018-01-01

    The turkey (Meleagris gallopavo) represents one of the few domestic animals of the New World. While current research points to distinct domestication centres in the Southwest USA and Mesoamerica, several questions regarding the number of progenitor populations, and the timing and intensity of turkey husbandry remain unanswered. This study applied ancient mitochondrial DNA and stable isotope (δ13C, δ15N) analysis to 55 archaeological turkey remains from Mexico to investigate pre-contact turkey exploitation in Mesoamerica. Three different (sub)species of turkeys were identified in the archaeological record (M. g. mexicana, M. g. gallopavo and M. ocellata), indicating the exploitation of diverse local populations, as well as the trade of captively reared birds into the Maya area. No evidence of shared maternal haplotypes was observed between Mesoamerica and the Southwest USA, in contrast with archaeological evidence for trade of other domestic products. Isotopic analysis indicates a range of feeding behaviours in ancient Mesoamerican turkeys, including wild foraging, human provisioning and mixed feeding ecologies. This variability in turkey diet decreases through time, with archaeological, genetic and isotopic evidence all pointing to the intensification of domestic turkey management and husbandry, culminating in the Postclassic period. PMID:29410864

  19. Diversity of management strategies in Mesoamerican turkeys: archaeological, isotopic and genetic evidence.

    Science.gov (United States)

    Manin, Aurelie; Corona-M, Eduardo; Alexander, Michelle; Craig, Abigail; Thornton, Erin Kennedy; Yang, Dongya Y; Richards, Michael; Speller, Camilla F

    2018-01-01

    The turkey ( Meleagris gallopavo ) represents one of the few domestic animals of the New World. While current research points to distinct domestication centres in the Southwest USA and Mesoamerica, several questions regarding the number of progenitor populations, and the timing and intensity of turkey husbandry remain unanswered. This study applied ancient mitochondrial DNA and stable isotope ( δ 13 C, δ 15 N) analysis to 55 archaeological turkey remains from Mexico to investigate pre-contact turkey exploitation in Mesoamerica. Three different (sub)species of turkeys were identified in the archaeological record ( M. g. mexicana , M. g. gallopavo and M. ocellata ), indicating the exploitation of diverse local populations, as well as the trade of captively reared birds into the Maya area. No evidence of shared maternal haplotypes was observed between Mesoamerica and the Southwest USA, in contrast with archaeological evidence for trade of other domestic products. Isotopic analysis indicates a range of feeding behaviours in ancient Mesoamerican turkeys, including wild foraging, human provisioning and mixed feeding ecologies. This variability in turkey diet decreases through time, with archaeological, genetic and isotopic evidence all pointing to the intensification of domestic turkey management and husbandry, culminating in the Postclassic period.

  20. Eimeria tenella: host specificity in gallinaceous birds.

    Science.gov (United States)

    Vetterling, J M

    1976-02-01

    Eight species representing 8 genera of gallinaceous birds were used: Alectoris graeca; Colinus virginianus; Coturnix coturnix; Gallus gallus; Meleagris gallopavo; Numidia meleagris; Pavo cristatus; Phasianus colchicus. Three week-old birds were dosed with sporulated oocysts of Eimeria tenella Beltsville strain. At 4, 24, 48, 72, 96, 120 and 144, and 168 hr after inoculation, 1-3 infected birds and uninoculated controls of each species were killed by cardiac exsanguination. Pieces of intestines were fixed and examined for stages of E. tenella as stained paraffin sections or indirect fluorescent antibody preparations. Oocyst counts were made in droppings collected for the first 6 days of the patent period. Sporozoites were found in the lamina propria of some birds of 5 species at 4 hr postinoculation, but no stages were found thereafter except in the breeds of G. gallus and A. gracea. At 144 and 168 hr postinoculation, a few macrogametes were found in the ceca of 2 A. gracea, but no oocysts were found in the feces. No statistical difference was found between the number of oocysts produced/bird in the breeds of G. gallus examined. It is evident from these observations the E. tenella did not complete its life cycle in several close phylogenetic relatives of G. gallus, even though in other studies this parasite was found to complete its life cycle in cell cultures derived from the same birds.

  1. Karyotypic evolution in the Galliformes: an examination of the process of karyotypic evolution by comparison of the molecular cytogenetic findings with the molecular phylogeny.

    Science.gov (United States)

    Shibusawa, M; Nishibori, M; Nishida-Umehara, C; Tsudzuki, M; Masabanda, J; Griffin, D K; Matsuda, Y

    2004-01-01

    To define the process of karyotypic evolution in the Galliformes on a molecular basis, we conducted genome-wide comparative chromosome painting for eight species, i.e. silver pheasant (Lophura nycthemera), Lady Amherst's pheasant (Chrysolophus amherstiae), ring-necked pheasant (Phasianus colchicus), turkey (Meleagris gallopavo), Western capercaillie (Tetrao urogallus), Chinese bamboo-partridge (Bambusicola thoracica) and common peafowl (Pavo cristatus) of the Phasianidae, and plain chachalaca (Ortalis vetula) of the Cracidae, with chicken DNA probes of chromosomes 1-9 and Z. Including our previous data from five other species, chicken (Gallus gallus), Japanese quail (Coturnix japonica) and blue-breasted quail (Coturnix chinensis) of the Phasianidae, guinea fowl (Numida meleagris) of the Numididae and California quail (Callipepla californica) of the Odontophoridae, we represented the evolutionary changes of karyotypes in the 13 species of the Galliformes. In addition, we compared the cytogenetic data with the molecular phylogeny of the 13 species constructed with the nucleotide sequences of the mitochondrial cytochrome b gene, and discussed the process of karyotypic evolution in the Galliformes. Comparative chromosome painting confirmed the previous data on chromosome rearrangements obtained by G-banding analysis, and identified several novel chromosome rearrangements. The process of the evolutionary changes of macrochromosomes in the 13 species was in good accordance with the molecular phylogeny, and the ancestral karyotype of the Galliformes is represented. Copyright 2004 S. Karger AG, Basel

  2. Host specificity of turkey and chicken Eimeria: controlled cross-transmission studies and a phylogenetic view.

    Science.gov (United States)

    Vrba, Vladimir; Pakandl, Michal

    2015-03-15

    Protozoan parasites of the Eimeria genus have undergone extensive speciation and are now represented by a myriad of species that are specialised to different hosts. These species are highly host-specific and usually parasitise single host species, with only few reported exceptions. Doubts regarding the strict host specificity were frequent in the original literature describing coccidia parasitising domestic turkeys. The availability of pure characterised lines of turkey and chicken Eimeria species along with the recently developed quantitative PCR identification of these species allowed to investigate the issue of host specificity using well-controlled cross-transmission experiments. Seven species of gallinaceous birds (Gallus gallus, Meleagris gallopavo, Alectoris rufa, Perdix perdix, Phasianus colchicus, Numida meleagris and Colinus virginianus) were inoculated with six species and strains of turkey Eimeria and six species of chicken coccidia and production of oocysts was monitored. Turkey Eimeria species E. dispersa, E. innocua and E. meleagridis could complete their development in the hosts from different genera or even different families. Comparison of phylogenetic positions of these Eimeria species according to 18S rDNA and COI showed that the phylogeny cannot explain the observed patterns of host specificity. These findings suggest that the adaptation of Eimeria parasites to foreign hosts is possible and might play a significant role in the evolution and diversification of this genus. Copyright © 2015 Elsevier B.V. All rights reserved.

  3. Amino Acid Levels in Muscle Tissue of Six Wild Feathered Species

    Directory of Open Access Journals (Sweden)

    Eva Straková

    2016-01-01

    Full Text Available The objective of this study was to determine and compare the levels of amino acids (AAs in breast and thigh muscles of six species of feathered game of the same age. The experiment involved the following species: wild turkey (Meleagris gallopavo, guinea fowl (Numida meleagris, chukar partridge (Alectoris chucar, Japanese quail (Coturnix coturnix japonica, common pheasant (Phasianus colchicus and grey partridge (Perdix perdix. The highest content of AAs was found in the chukar partridge (breast: 815.7 ± 47.71 g/kg; thigh: 771.4 ± 107.0 g/kg, on a dry matter basis, the lowest levels of AAs were found in Japanese quail (breast: 734.2 ± 45.07 g/kg and grey partridge (thigh: 614.9 ± 49.66 g/kg. In all examined species, the level of histidine in breast muscles differed (P ≤ 0.01 from that in thigh muscles. In all investigated species, the levels of essential AAs in breast muscles were higher (P ≤ 0.01 than those in thigh muscles, whereas the levels of non-essential AAs in breast muscles were lower (P ≤ 0.01 than those in thigh muscles. Breast muscles are therefore more valuable than thigh muscles because of the content of essential AAs.

  4. Energy value of meat in selected species of feathered game

    Directory of Open Access Journals (Sweden)

    František Vitula

    2011-01-01

    Full Text Available The aim of this work was to compare gross energy (GE in breast and thigh muscles in the following six species of feathered game reared in Europe: guineafowl (Numida meleagris, common pheasant (Phasianus colchicus, Japanese quail (Coturnix coturnix japonica, chukar (Alectoris chucar, grey partridge (Perdix perdix and wild turkey (Meleagris gallopavo. Calorimetric analysis revealed significant (P ≤ 0.05 and highly significant (P ≤ 0.01 differences between individual species in the content of energy in breast and thigh muscles. The highest content of energy (recalculated to dry matter was found in breast muscles from wild turkey (24.75 MJ·kg-1 and Japanese quail (24.57 MJ·kg-1 whereas the highest content of energy (recalculated to dry matter in thigh muscles was found in Japanese quail and grey partridge. Highly significant (P ≤ 0.01 differences in the energy content were also found between breast and thigh muscles in all studied game species except for wild turkey. Differences in the content of energy in muscles between individual species occur mainly due to different contents of fat in muscles. This is also confirmed by high correlation coefficients between the content of energy and the content of fat in breast (r = 0.912 and thigh muscles (r = 0.878. Our study provides more specific data on the amount of energy in muscles of major species of feathered game reared in Europe and significantly extends current knowledge in this field.

  5. Survey for antibodies to infectious bursal disease virus serotype 2 in wild turkeys and Sandhill Cranes of Florida, USA.

    Science.gov (United States)

    Candelora, Kristen L; Spalding, Marilyn G; Sellers, Holly S

    2010-07-01

    Captive-reared Whooping Cranes (Grus americana) released into Florida for the resident reintroduction project experienced unusually high mortality and morbidity during the 1997-98 and 2001-02 release seasons. Exposure to infectious bursal disease virus (IBDV) serotype 2 as evidenced by seroconversion was suspected to be the factor that precipitated these mortality events. Very little is known about the incidence of IBD in wild bird populations. Before this study, natural exposure had not been documented in wild birds of North America having no contact with captive-reared cranes, and the prevalence and transmission mechanisms of the virus in wild birds were unknown. Sentinel chickens (Gallus gallus) monitored on two Whooping Crane release sites in central Florida, USA, during the 2003-04 and 2004-05 release seasons seroconverted, demonstrating natural exposure to IBDV serotype 2. Blood samples collected from Wild Turkeys (Meleagris gallopavo) and Sandhill Cranes (Grus canadensis) in eight of 21 counties in Florida, USA, and one of two counties in southern Georgia, USA, were antibody-positive for IBDV serotype 2, indicating that exposure from wild birds sharing habitat with Whooping Cranes is possible. The presence of this virus in wild birds in these areas is a concern for the resident flock of Whooping Cranes because they nest and raise their chicks in Florida, USA. However, passively transferred antibodies may protect them at this otherwise vulnerable period in their lives.

  6. Feather barbs as a good source of mtDNA for bird species identification in forensic wildlife investigations.

    Science.gov (United States)

    Speller, Camilla F; Nicholas, George P; Yang, Dongya Y

    2011-07-28

    The ability to accurately identify bird species is crucial for wildlife law enforcement and bird-strike investigations. However, such identifications may be challenging when only partial or damaged feathers are available for analysis. By applying vigorous contamination controls and sensitive PCR amplification protocols, we found that it was feasible to obtain accurate mitochondrial (mt)DNA-based species identification with as few as two feather barbs. This minimally destructive DNA approach was successfully used and tested on a variety of bird species, including North American wild turkey (Meleagris gallopavo), Canada goose (Branta canadensis), blue heron (Ardea herodias) and pygmy owl (Glaucidium californicum). The mtDNA was successfully obtained from 'fresh' feathers, historic museum specimens and archaeological samples, demonstrating the sensitivity and versatility of this technique. By applying appropriate contamination controls, sufficient quantities of mtDNA can be reliably recovered and analyzed from feather barbs. This previously overlooked substrate provides new opportunities for accurate DNA species identification when minimal feather samples are available for forensic analysis.

  7. Turkey-hen amino acid composition of brain and eyes

    International Nuclear Information System (INIS)

    Adeyeye, E.I.

    2015-01-01

    The amino acids composition of the brain and eyes of the mature Turkey-hen (Meleagris gallopavo L.), were determined on dry weight basis. Total essential amino acids ranged from 35.1-36.0 g/100 g as 49.5-49.8% of the total amino acids. The amino acid score showed that lysine ranged from 0.76-0.91 (on whole hen.s egg comparison), 0.85-1.03 (on provisional essential amino acid scoring pattern), and 0.81-0.98 (on suggested requirement of the essential amino acid of a preschool child). The predicted protein efficiency ratio was 1.94-2.41, whilst essential amino acid index range was 1.06-1.08 and the calculated isoelectric point range was 3.97-4.18. The correlation coefficient (rxy) was positively high and significant at r = 0.01 for the total amino acids, amino acid scores (on the whole hen.s egg comparisons made) and the isoelectric point. On the whole, the eyes were better in 12/18 or 66.7% parameters of the amino acids than the brain of Turkey-Hen. (author)

  8. Molecular phylogeny of some avian species using Cytochrome b gene sequence analysis

    Science.gov (United States)

    Awad, A; Khalil, S. R; Abd-Elhakim, Y. M

    2015-01-01

    Veritable identification and differentiation of avian species is a vital step in conservative, taxonomic, forensic, legal and other ornithological interventions. Therefore, this study involved the application of molecular approach to identify some avian species i.e. Chicken (Gallus gallus), Muskovy duck (Cairina moschata), Japanese quail (Coturnix japonica), Laughing dove (Streptopelia senegalensis), and Rock pigeon (Columba livia). Genomic DNA was extracted from blood samples and partial sequence of the mitochondrial cytochrome b gene (358 bp) was amplified and sequenced using universal primers. Sequences alignment and phylogenetic analyses were performed by CLC main workbench program. The obtained five sequences were deposited in GenBank and compared with those previously registered in GenBank. The similarity percentage was 88.60% between Gallus gallus and Coturnix japonica and 80.46% between Gallus gallus and Columba livia. The percentage of identity between the studied species and GenBank species ranged from 77.20% (Columba oenas and Anas platyrhynchos) to 100% (Gallus gallus and Gallus sonneratii, Coturnix coturnix and Coturnix japonica, Meleagris gallopavo and Columba livia). Amplification of the partial sequence of mitochondrial cytochrome b gene proved to be practical for identification of an avian species unambiguously. PMID:27175180

  9. Eimeria atlapetesi nom. nov., a replacement name for Eimeria pileata Soriano-Vargas et al., 2015 (Apicomplexa: Eimeriidae), preoccupied by Eimeria pileata Straneva and Kelley, 1979 (Apicomplexa: Eimeriidae), with observations on histopathology and phylogenetic analysis.

    Science.gov (United States)

    Soriano-Vargas, Edgardo; Salgado-Miranda, Celene; Zepeda-Velázquez, Andrea Paloma; Medina, Juan Pablo; Janczur, Mariusz Krzysztof; González-Gómez, Maricruz; Flores-Valle, Izanami Tereira; Berto, Bruno Pereira; Lopes, Carlos Wilson Gomes

    2017-01-31

    Eimeria pileata Soriano-Vargas, Medina, Salgado-Miranda, García-Conejo, Galindo-Sánchez, Janczur, Berto and Lopes, 2015 is a junior homonym of Eimeria pileata Straneva and Kelley, 1979 and needs to be replaced. This coccidium was described from a rufous-capped brush finch Atlapetes pileatus Wagler in the Nevado de Toluca Natural Protected Area, Mexico. Thus, to maintain the original intent of the specific epithet derived from the scientific name of the type-host, the name Eimeria atlapetesi nom. nov. is proposed as a replacement name. Additionally, the current work reports another rufous-capped brush finch A. pileatus parasitized by E. atlapetesi in co-infection with an Isospora sp., providing observations of histopathology and phylogenetic analysis of 18S ribosomal RNA (rRNA) gene from E. atlapetesi. Endogenous forms of E. atlapetesi and Isospora sp. were observed in intestinal sections. Few oocysts of Isospora sp. were observed; therefore they were not morphologically or molecularly identified. In return, E. atlapetesi was identified and it was phylogenetically close to Eimeria dispersa Tyzzer, 1929 from the domestic turkey Meleagris gallopavo Linnaeus.

  10. Influence of season and sex on hemato-biochemical traits in adult turkeys under arid tropical environment

    Directory of Open Access Journals (Sweden)

    Anil Gattani

    2016-05-01

    Full Text Available Aim: The objective of this study was to evaluate the effect of season and sex on hemato-biochemical parameters of turkey (Meleagris gallopavo in the arid tropical environment. Materials and Methods: The experiment was conducted on 20-week old turkeys consisting of 20 males and 20 females. Blood was collected from all turkeys during January and May. Hemoglobin (Hb, red blood cell (RBC, packed cell volume (PCV, mean corpuscular volume (MCV, mean corpuscular hemoglobin (MCH, and mean corpuscular hemoglobin concentration (MCHC were estimated in whole blood and glucose, protein, albumin, globulin, A/G ratio, calcium, phosphorus, alanine aminotransferase (ALT, and aspartate aminotransferase (AST in serum. Result: Season has significant (p<0.05 effect on Hb concentration, RBC, and PCV in both male and female. Male has significantly higher (p<0.05 Hb concentration, RBC, and PCV. There is no significant effect of sex, and season was observed on MCV, MCH, and MCHC. Glucose, protein, albumin, globulin, and A/G ratio were significantly (p<0.05 affected by season and sex. AST and ALT were significantly (p<0.05 affected by season in both sexes. There is no significant difference was recorded on calcium, phosphorus due to season and sex. Conclusion: Under arid tropical environment, turkey hemato-biochemical parameters are influenced by both sex and season.

  11. Clinical and Pathologic Characterization of an Outbreak of Highly Pathogenic Avian Influenza H7N8 in Commercial Turkeys in Southern Indiana.

    Science.gov (United States)

    Burcham, Grant N; Ramos-Vara, José A; Murphy, Duane A

    2017-09-01

    Highly pathogenic avian influenza (HPAI) is a systemic lethal disease of poultry caused by several subtypes of influenza A virus and classified on the basis of serologic reactions to hemagglutinin and neuraminidase surface glycoproteins. In January 2016, a novel subtype of HPAI-H7N8-was diagnosed in a commercial turkey (Meleagris gallopavo) flock in southern Indiana. Clinical signs and history included increased mortality, dyspnea, head tremors, recumbency, and somnolent or unaware birds. Postmortem examination of six recently dead birds showed red-tinged mucous in the choana and trachea and marked pulmonary edema. Histologic lesions in the brain included severe, multifocal lymphohistiocytic meningoencephalitis with foci of malacia, neuronal necrosis, and neuronophagia. All anatomic locations of the brain were affected, although histologic changes in the cerebellum were considered mild. Other histologic lesions included pulmonary congestion and edema, splenic congestion and lymphoid depletion, fibrinoid necrosis of vessels within the spleen, and multifocal pancreatic acinar necrosis. Immunohistochemistry (IHC) was weakly positive for influenza A in the brain; IHC was negative in other tissues tested. The clinical and pathologic characteristics of this case matched previously published material concerning HPAI and add to instances of known or suspected mutation of a low pathogenic virus to a highly pathogenic virus.

  12. Hepatic Transcriptome Responses of Domesticated and Wild Turkey Embryos to Aflatoxin B1

    Directory of Open Access Journals (Sweden)

    Melissa S. Monson

    2016-01-01

    Full Text Available The mycotoxin, aflatoxin B1 (AFB1 is a hepatotoxic, immunotoxic, and mutagenic contaminant of food and animal feeds. In poultry, AFB1 can be maternally transferred to embryonated eggs, affecting development, viability and performance after hatch. Domesticated turkeys (Meleagris gallopavo are especially sensitive to aflatoxicosis, while Eastern wild turkeys (M. g. silvestris are likely more resistant. In ovo exposure provided a controlled AFB1 challenge and comparison of domesticated and wild turkeys. Gene expression responses to AFB1 in the embryonic hepatic transcriptome were examined using RNA-sequencing (RNA-seq. Eggs were injected with AFB1 (1 μg or sham control and dissected for liver tissue after 1 day or 5 days of exposure. Libraries from domesticated turkey (n = 24 and wild turkey (n = 15 produced 89.2 Gb of sequence. Approximately 670 M reads were mapped to a turkey gene set. Differential expression analysis identified 1535 significant genes with |log2 fold change| ≥ 1.0 in at least one pair-wise comparison. AFB1 effects were dependent on exposure time and turkey type, occurred more rapidly in domesticated turkeys, and led to notable up-regulation in cell cycle regulators, NRF2-mediated response genes and coagulation factors. Further investigation of NRF2-response genes may identify targets to improve poultry resistance.

  13. A nuclear DNA-based species determination and DNA quantification assay for common poultry species.

    Science.gov (United States)

    Ng, J; Satkoski, J; Premasuthan, A; Kanthaswamy, S

    2014-12-01

    DNA testing for food authentication and quality control requires sensitive species-specific quantification of nuclear DNA from complex and unknown biological sources. We have developed a multiplex assay based on TaqMan® real-time quantitative PCR (qPCR) for species-specific detection and quantification of chicken (Gallus gallus), duck (Anas platyrhynchos), and turkey (Meleagris gallopavo) nuclear DNA. The multiplex assay is able to accurately detect very low quantities of species-specific DNA from single or multispecies sample mixtures; its minimum effective quantification range is 5 to 50 pg of starting DNA material. In addition to its use in food fraudulence cases, we have validated the assay using simulated forensic sample conditions to demonstrate its utility in forensic investigations. Despite treatment with potent inhibitors such as hematin and humic acid, and degradation of template DNA by DNase, the assay was still able to robustly detect and quantify DNA from each of the three poultry species in mixed samples. The efficient species determination and accurate DNA quantification will help reduce fraudulent food labeling and facilitate downstream DNA analysis for genetic identification and traceability.

  14. Experimental Mycoplasma gallisepticum infections in captive-reared wild turkeys

    Science.gov (United States)

    Rocke, Tonie E.; Yuill, Thomas M.; Amundson, Terry E.

    1988-01-01

    The effects of Mycoplasma gallisepticum (MG) infections on egg production, fertility, and hatchability were studied in captive-reared wild turkeys (Meleagris gallopavo). Three groups of adult birds, each consisting of four hens and two toms, were exposed to MG by the respiratory route at the beginning of their breeding season. Fourteen control birds received sterile growth medium. Although no mortality of infected or control birds occurred, egg production during the first breeding season after infection was reduced. The mean number of eggs/hen/day produced by infected groups the first breeding season postexposure (PE) was significantly lower than the control value. The mean number of eggs produced daily by the same hens 1 yr later was unaffected by MG infection. The pecentage of fertile eggs produced by infected groups was slightly reduced in both the first and second breeding seasons PE. Hatchability of fertile eggs from infected hens was significantly lower than eggs from control hens. Productivity may be impaired if MG infections occur in free-ranging wild turkey populations.

  15. Effect of Rearing Systems on Reproductive Performance of Turkey

    Directory of Open Access Journals (Sweden)

    M AnnaAnandh

    2012-08-01

    Full Text Available The objective of this study was to determine the effect of rearing systems on reproductive performance of turkey (Meleagris gallopavo. A total of 180 Beltsville Small White and Board Breasted Bronze turkeys were taken for the study and reared under three different rearing system viz. intensive system (full confinement, semi-intensive system (partial confinement and partial day scavenging and free range system (all-day scavenging. Average egg weight (g, percentage of infertile eggs, embryonic mortalities, total egg hatchability, fertile egg hatchability, fertility and poults survivability values were significantly (P>0.01 higher in turkeys reared under intensive system of management followed by semi intensive system and free range system of management. The highest percentage of dead in shell was found in intensive system and was did not differ significantly from semi intensive and free range system. Hatched weight of poults (g between semi intensive and intensive system did not differ significantly between them, but both groups found statistically significant (P>0.01 from free range system. From the study, it is concluded that higher reproductive performance was obtained in intensive system of management followed by semi intensive and free range system of management. [Vet. World 2012; 5(4.000: 226-229

  16. Prevalence and burden of gastrointestinal helminths in wild and domestic guineafowls (Numida meleagris in the Southern Province of Zambia

    Directory of Open Access Journals (Sweden)

    King Shimumbo Nalubamba

    2015-08-01

    Conclusions: Syngamus trachea, Streptocara pectinifera and Acuaria spiralis are reported for the first time in domestic poultry in Zambia. This study represents the first comparative study of helminths in domestic and wild guineafowls at an interface area and adds to the knowledge base in a discipline where a dearth currently exists.

  17. Characterization of pyranose dehydrogenase from Agaricus meleagris and its application in the C-2 specific conversion of D-galactose

    Czech Academy of Sciences Publication Activity Database

    Sygmund, Ch.; Kittl, R.; Volc, Jindřich; Halada, Petr; Kubátová, Elena; Haltrich, D.; Peterbauer, C.

    2008-01-01

    Roč. 133, č. 3 (2008), s. 334-342 ISSN 0168-1656 Grant - others:CZ(CZ) program KONTAKT ČR-Rakousko Institutional research plan: CEZ:AV0Z50200510 Keywords : pyranose dehydrogenase * sugar oxidoreductase * galactose coversion Subject RIV: EE - Microbiology, Virology Impact factor: 2.748, year: 2008

  18. Ca(2+)-dependent nonspecific permeability of the inner membrane of liver mitochondria in the guinea fowl (Numida meleagris).

    Science.gov (United States)

    Vedernikov, Aleksander A; Dubinin, Mikhail V; Zabiakin, Vladimir A; Samartsev, Victor N

    2015-06-01

    This comparative study presents the results of the induction of Ca(2+)-dependent nonspecific permeability of the inner membrane (pore opening) of rat and guinea fowl liver mitochondria by mechanisms that are both sensitive and insensitive to cyclosporin A (CsA). It was established that energized rat and guinea fowl liver mitochondria incubated with 1 mM of inorganic phosphate (Pi) are capable of swelling upon addition of at least 125 and 875 nmol of CaCl2 per 1 mg protein, respectively. Under these conditions, the Ca(2+) release from the mitochondria of these animals and a drop in Δψ are observed. All of these processes are inhibited by 1 μM of CsA. FCCP, causing organelle de-energization, induces pore opening in rat and guinea fowl liver mitochondria upon addition of 45 и 625 nmol of CaCl2 per 1 mg protein, respectively. These results suggest the existence of a CsA-sensitive mechanism for the induction of Ca(2+)-dependent pores in guinea fowl liver mitochondria, which has been reported in rat liver mitochondria. However, guinea fowl liver mitochondria have a significantly greater resistance to Ca(2+) as a pore inducer compared to rat liver mitochondria. It was found that the addition of α,ω-hexadecanedioic acid (HDA) to rat and guinea fowl liver mitochondria incubated with CsA and loaded with Ca(2+) causes organelle swelling and Ca(2+) release from the matrix. It is assumed that in contrast to the CsA-sensitive pore, the CsA-insensitive pore induced by HDA in the inner membrane of guinea fowl liver mitochondria, as well as in rat liver mitochondria, is lipid in nature.

  19. Vertebrate host specificity and experimental vectors of Plasmodium (Novyella) kempi sp. n. from the eastern wild turkey in Iowa.

    Science.gov (United States)

    Christensen, B M; Barnes, H J; Rowley, W A

    1983-07-01

    Vertebrate host specificity, experimental laboratory vectors, and a description of Plasmodium (Novyella) kempi sp. n. from eastern wild turkeys (Meleagris gallopavo silvestris Vieillot) in Iowa are presented. Plasmodium kempi is infective for domestic turkeys, bobwhites (Colinus virginianus), chukars (Alectoris graeca), guinea fowl (Numida meleagris), peacocks (Pavo cristatus), and canaries (Serinus canaria), produces a transient infection in mallards (Anas platyrhynchos) and domestic geese (Anser anser), but will not infect ring-necked pheasants (Phasianus colchicus), pigeons (Columba livia), Japanese quail (Coturnix coturnix), leghorn white chickens (Gallus gallus), or starlings (Sturnus vulgaris). Oocysts and (or) sporozoites were recovered from 68% (84/124) and 98% (60/61) of the Culex pipiens pipiens and C. tarsalis examined, respectively. Oocysts developed faster and sporozoites invaded the salivary glands sooner in C. tarsalis (6 days) than in C. p. pipiens (7 days). Culex tarsalis transmitted P. kempi more effectively than C. p. pipiens, although both species were capable of transmitting the parasite by natural feeding. Oocysts developed and sporozoites also were produced in C. restuans, but its ability to transmit the parasite was not determined. Aedes aegypti (Rockefeller strain) and A. triseriatus were refractive to P. kempi. Plasmodium kempi produces trophozoites with large refractile globules and fine cytoplasmic extensions, mature schizonts in the form of a condensed fan containing four to eight nuclei (usually 5), and elongate gametocytes with irregular borders. All stages are confined almost exclusively to mature erythrocytes, with no effect on host cell size or position of host cell nucleus. Plasmodium kempi is most similar morphologically to P. (Novyella) hexamerium and P. (Novyella) vaughani. It differs from P. hexamerium in having large refractile globules in trophozoites and immature schizonts, an inability to infect starlings, an absence of

  20. Two agricultural production data libraries for risk assessment models

    International Nuclear Information System (INIS)

    Baes, C.F. III; Shor, R.W.; Sharp, R.D.; Sjoreen, A.L.

    1985-01-01

    Two data libraries based on the 1974 US Census of Agriculture are described. The data packages (AGDATC and AGDATG) are available from the Radiation Shielding Information Center (RSIC), Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831. Agricultural production and land-use information by county (AGDATC) or by 1/2 by 1/2 degree longitude-latitude grid cell (AGDATG) provide geographical resolution of the data. The libraries were designed for use in risk assessment models that simulate the transport of radionuclides from sources of airborne release through food chains to man. However, they are also suitable for use in the assessment of other airborne pollutants that can affect man from a food ingestion pathway such as effluents from synfuels or coal-fired power plants. The principal significance of the data libraries is that they provide default location-specific food-chain transport parameters when site-specific information are unavailable. Plant food categories in the data libraries include leafy vegetables, vegetables and fruits exposed to direct deposition of airborne pollutants, vegetables and fruits protected from direct deposition, and grains. Livestock feeds are also tabulated in four categories: pasture, grain, hay, and silage. Pasture was estimated by a material balance of cattle and sheep inventories, forage feed requirements, and reported harvested forage. Cattle (Bos spp.), sheep (Ovis aries), goat (Capra hircus), hog (Sus scrofa), chicken (Gallus domesticus), and turkey (Meleagris gallopavo) inventories or sales are also tabulated in the data libraries and can be used to provide estimates of meat, eggs, and milk production. Honey production also is given. Population, irrigation, and meteorological information are also listed

  1. Reduced population variance in strontium isotope values informs domesticated turkey use at Chaco Canyon, New Mexico, USA

    Science.gov (United States)

    Grimstead, Deanna N; Reynolds, Amanda C; Hudson, Adam M; Akins, Nancy J; Betancourt, Julio L.

    2016-01-01

    Traditionally strontium isotopes (87Sr/86Sr) have been used as a sourcing tool in numerous archaeological artifact classes. The research presented here demonstrates that 87Sr/86Srbioapatite ratios also can be used at a population level to investigate the presence of domesticated animals and methods of management. The proposed methodology combines ecology, isotope geochemistry, and behavioral ecology to assess the presence and nature of turkey (Meleagris gallopavo) domestication. This case study utilizes 87Sr/86Srbioapatite ratios from teeth and bones of archaeological turkey, deer (Odocoileus sp.), lagomorph (Lepus sp. and Sylvilagus sp.), and prairie-dog (Cynomys sp.) from Chaco Canyon, New Mexico, U.S.A. (ca. A.D. 800 – 1250). Wild deer and turkey from the southwestern U.S.A. have much larger home ranges and dispersal behaviors (measured in kilometers) when compared to lagomorphs and prairie dogs (measured in meters). Hunted deer and wild turkey from archaeological contexts at Chaco Canyon are expected to have a higher variance in their 87Sr/86Srbioapatite ratios, when compared to small range taxa (lagomorphs and prairie dogs). Contrary to this expectation, 87Sr/86Srbioapatite values of turkey bones from Chacoan assemblages have a much lower variance than deer and are similar to that of smaller mammals. The sampled turkey values show variability most similar to lagomorphs and prairie dogs, suggesting the turkeys from Chaco Canyon were consuming a uniform diet and/or were constrained within a limited home range, indicating at least proto-domestication. The population approach has wide applicability for evaluating the presence and nature of domestication when combined with paleoecology and behavioral ecology in a variety of animals and environments.

  2. Aflatoxicosis: Lessons from Toxicity and Responses to Aflatoxin B1 in Poultry

    Directory of Open Access Journals (Sweden)

    Melissa S. Monson

    2015-09-01

    Full Text Available This review is a comprehensive introduction to the effects of poultry exposure to the toxic and carcinogenic mycotoxin aflatoxin B1 (AFB1. The relationship between AFB1 sensitivity and metabolism, major direct and indirect effects of AFB1, recent studies of gene expression and transcriptome responses to exposure, and mitigation strategies to reduce toxicity are discussed. Exposure to AFB1 primarily occurs by consumption of contaminated corn, grain or other feed components. Low levels of residual AFB1 in poultry feeds can cause reduction in growth, feed conversion, egg production, and compromised immune functions, resulting in significant economic costs to producers. Thus, AFB1 acts as a “force multiplier” synergizing the adverse effects of microbial pathogens and other agents, and factors detrimental to poultry health. Domestic turkeys (Meleagris gallopavo are one of the most sensitive animals known to AFB1 due, in large part, to a combination of efficient hepatic bioactivation by cytochromes P450 1A5 and 3A37, and deficient hepatic glutathione-S-transferase (GST-mediated detoxification. Because of their sensitivity, turkeys are a good model to investigate chemopreventive treatments and feed additives for their ability to reduce AFB1 toxicity. Transcriptome analysis (RNA-seq of turkey poults (liver and spleen has identified AFB1-induced gene expression changes in pathways of apoptosis, carcinogenesis, lipid regulation, antimicrobial activity, cytotoxicity and antigen presentation. Current research focuses on further identifying the molecular mechanisms underlying AFB1 toxicity with the goal of reducing aflatoxicosis and improving poultry health.

  3. Comparative Response of the Hepatic Transcriptomes of Domesticated and Wild Turkey to Aflatoxin B1

    Directory of Open Access Journals (Sweden)

    Kent M. Reed

    2018-01-01

    Full Text Available The food-borne mycotoxin aflatoxin B1 (AFB1 poses a significant risk to poultry, which are highly susceptible to its hepatotoxic effects. Domesticated turkeys (Meleagris gallopavo are especially sensitive, whereas wild turkeys (M. g. silvestris are more resistant. AFB1 toxicity entails bioactivation by hepatic cytochrome P450s to the electrophilic exo-AFB1-8,9-epoxide (AFBO. Domesticated turkeys lack functional hepatic GST-mediated detoxification of AFBO, and this is largely responsible for the differences in resistance between turkey types. This study was designed to characterize transcriptional changes induced in turkey livers by AFB1, and to contrast the response of domesticated (susceptible and wild (more resistant birds. Gene expression responses to AFB1 were examined using RNA-sequencing. Statistically significant differences in gene expression were observed among treatment groups and between turkey types. Expression analysis identified 4621 genes with significant differential expression (DE in AFB1-treated birds compared to controls. Characterization of DE transcripts revealed genes dis-regulated in response to toxic insult with significant association of Phase I and Phase II genes and others important in cellular regulation, modulation of apoptosis, and inflammatory responses. Constitutive expression of GSTA3 was significantly higher in wild birds and was significantly higher in AFB1-treated birds when compared to controls for both genetic groups. This pattern was also observed by qRT-PCR in other wild and domesticated turkey strains. Results of this study emphasize the differential response of these genetically distinct birds, and identify genes and pathways that are differentially altered in aflatoxicosis.

  4. Hematozoan parasites of Rio Grande wild turkeys from southern Texas (USA)

    Science.gov (United States)

    Castle, Marc D.; Christensen, Beth A.; Rocke, Tonie E.

    1988-01-01

    One hundred twenty-three of 300 blood samples (41%) taken from Rio Grande wild turkeys (Meleagris gallopavo intermedia) from three locations in southern Texas (Welder Wildlife Refuge, Chaparrosa Ranch, and Campo Alegre Ranch) and subinoculated into domestic broad-breasted white turkey poults were positive for a Plasmodium (Novyella) sp. Analysis of blood films from 350 turkeys revealed Haemoproteus meleagridis in 76% of the birds. A significantly greater mean parasite intensity was observed in birds from Welder Wildlife Refuge. Birds from the Campo Alegre Ranch exhibited a significantly higher prevalence of H. meleagridis than birds from Chaparrosa. The Plasmodium sp. was infective for canaries (Serinus canaria), bobwhites (Colinus virginianus), and ring-necked pheasants (Phasianus colchicus), but would not produce infection in white leghorn chickens (Gallus gallus) or Coturnix quail (Coturnix coturnix). Attempts to infect Culex tarsalis and C. pipiens pipiens were unsuccessful. Asexual erythrocytic synchrony was not observed when blood-induced infections were monitored in two domestic turkey poults every 4 hr for 72 hr. Exoerythrocytic stages were not found upon examination of impression smears and tissue samples taken from brain, liver, spleen, kidney, lung, and bone marrow. The Plasmodium sp. is most similar morphologically to three species in the subgenus Novyella, P. hexamerium, P. vaughani, and P. kempi. The most striking similarities are to P. hexamerium, and involve mean merozoite number, erythrocytic schizont location, and vertebrate host susceptibility. It differs from P. vaughani in being able to infect turkeys and in type of parasitized erythrocytes. Differences to P. kempi include mean merozoite number, and ability to infect pheasants, and its inability to develop inC. pipiens and C. tarsalis.

  5. Nutrient digestibility of Mucuna (Mucuna pruriens var. utilis) bean in guinea fowl (Numida meleagris, L): Effects of heat treatment and levels of incorporation in diets.

    Science.gov (United States)

    Dahouda, M; Toléba, S S; Youssao, A K I; Hambuckers, A; Dangou-Sapoho, R; Martin, G B; Fillet, M; Hornick, J-L

    2009-09-01

    1. Mucuna pruriens var. utilis is a legume, the seeds of which are scarcely used in animal diets owing to their high content of 3,4-dihydroxy-L-phenylalanine (L-Dopa). 2. Experiments were conducted on guinea fowl to assess the effects of two types of heat processing (cooking and toasting) on chemical composition and nutrient digestibility of Mucuna seeds offered alone or incorporated at three concentrations (40, 120 or 200 g/kg) in complete diets. 3. Diets containing 200 g/kg seeds had more crude fibre and less ether extract. L-Dopa content increased with the amount of Mucuna inclusion. Cooking reduced markedly L-Dopa content while toasting had no effect. When fed alone, Mucuna seeds dramatically decreased feed intake. 4. Feed intake (FI) and body weight gain (BWG) were not influenced by the complete diets. Cooking significantly increased crude fibre digestibility. 5. It is suggested that cracked and cooked Mucuna bean can be incorporated at a safe level of 120 g/kg in complete diets for guinea fowl production.

  6. Using dynamic Brownian bridge movement modelling to measure temporal patterns of habitat selection.

    Science.gov (United States)

    Byrne, Michael E; Clint McCoy, J; Hinton, Joseph W; Chamberlain, Michael J; Collier, Bret A

    2014-09-01

    Accurately describing animal space use is vital to understanding how wildlife use habitat. Improvements in GPS technology continue to facilitate collection of telemetry data at high spatial and temporal resolutions. Application of the recently introduced dynamic Brownian bridge movement model (dBBMM) to such data is promising as the method explicitly incorporates the behavioural heterogeneity of a movement path into the estimated utilization distribution (UD). Utilization distributions defining space use are normally estimated for time-scales ranging from weeks to months, obscuring much of the fine-scale information available from high-volume GPS data sets. By accounting for movement heterogeneity, the dBBMM provides a rigorous, behaviourally based estimate of space use between each set of relocations. Focusing on UDs generated between individual sets of locations allows us to quantify fine-scale circadian variation in habitat use. We used the dBBMM to estimate UDs bounding individual time steps for three terrestrial species with different life histories to illustrate how the method can be used to identify fine-scale variations in habitat use. We also demonstrate how dBBMMs can be used to characterize circadian patterns of habitat selection and link fine-scale patterns of habitat use to behaviour. We observed circadian patterns of habitat use that varied seasonally for a white-tailed deer (Odocoileus virginianus) and coyote (Canis latrans). We found seasonal patterns in selection by the white-tailed deer and were able to link use of conifer forests and agricultural fields to behavioural state of the coyote. Additionally, we were able to quantify the date in which a Rio Grande wild turkey (Meleagris gallopavo intermedia) initiated laying as well as when during the day, she was most likely to visit the nest site to deposit eggs. The ability to quantify circadian patterns of habitat use may have important implications for research and management of wildlife

  7. Estimates of soil ingestion by wildlife

    Science.gov (United States)

    Beyer, W.N.; Connor, E.E.; Gerould, S.

    1994-01-01

    Many wildlife species ingest soil while feeding, but ingestion rates are known for only a few species. Knowing ingestion rates may be important for studies of environmental contaminants. Wildlife may ingest soil deliberately, or incidentally, when they ingest soil-laden forage or animals that contain soil. We fed white-footed mice (Peromyscus leucopus) diets containing 0-15% soil to relate the dietary soil content to the acid-insoluble ash content of scat collected from the mice. The relation was described by an equation that required estimates of the percent acid-insoluble ash content of the diet, digestibility of the diet, and mineral content of soil. We collected scat from 28 wildlife species by capturing animals, searching appropriate habitats for scat, or removing material from the intestines of animals collected for other purposes. We measured the acid-insoluble ash content of the scat and estimated the soil content of the diets by using the soil-ingestion equation. Soil ingestion estimates should be considered only approximate because they depend on estimated rather than measured digestibility values and because animals collected from local populations at one time of the year may not represent the species as a whole. Sandpipers (Calidris spp.), which probe or peck for invertebrates in mud or shallow water, consumed sediments at a rate of 7-30% of their diets. Nine-banded armadillo (Dasypus novemcinctus, soil = 17% of diet), American woodcock (Scolopax minor, 10%), and raccoon (Procyon lotor, 9%) had high rates of soil ingestion, presumably because they ate soil organisms. Bison (Bison bison, 7%), black-tailed prairie dog (Cynomys ludovicianus, 8%), and Canada geese (Branta canadensis, 8%) consumed soil at the highest rates among the herbivores studied, and various browsers studied consumed little soil. Box turtle (Terrapene carolina, 4%), opossum (Didelphis virginiana, 5%), red fox (Vulpes vulpes, 3%), and wild turkey (Meleagris gallopavo, 9%) consumed soil

  8. Complete mitochondrial genome sequences from five Eimeria species (Apicomplexa; Coccidia; Eimeriidae) infecting domestic turkeys.

    Science.gov (United States)

    Ogedengbe, Mosun E; El-Sherry, Shiem; Whale, Julia; Barta, John R

    2014-07-17

    Clinical and subclinical coccidiosis is cosmopolitan and inflicts significant losses to the poultry industry globally. Seven named Eimeria species are responsible for coccidiosis in turkeys: Eimeria dispersa; Eimeria meleagrimitis; Eimeria gallopavonis; Eimeria meleagridis; Eimeria adenoeides; Eimeria innocua; and, Eimeria subrotunda. Although attempts have been made to characterize these parasites molecularly at the nuclear 18S rDNA and ITS loci, the maternally-derived and mitotically replicating mitochondrial genome may be more suited for species level molecular work; however, only limited sequence data are available for Eimeria spp. infecting turkeys. The purpose of this study was to sequence and annotate the complete mitochondrial genomes from 5 Eimeria species that commonly infect the domestic turkey (Meleagris gallopavo). Six single-oocyst derived cultures of five Eimeria species infecting turkeys were PCR-amplified and sequenced completely prior to detailed annotation. Resulting sequences were aligned and used in phylogenetic analyses (BI, ML, and MP) that included complete mitochondrial genomes from 16 Eimeria species or concatenated CDS sequences from each genome. Complete mitochondrial genome sequences were obtained for Eimeria adenoeides Guelph, 6211 bp; Eimeria dispersa Briston, 6238 bp; Eimeria meleagridis USAR97-01, 6212 bp; Eimeria meleagrimitis USMN08-01, 6165 bp; Eimeria gallopavonis Weybridge, 6215 bp; and Eimeria gallopavonis USKS06-01, 6215 bp). The order, orientation and CDS lengths of the three protein coding genes (COI, COIII and CytB) as well as rDNA fragments encoding ribosomal large and small subunit rRNA were conserved among all sequences. Pairwise sequence identities between species ranged from 88.1% to 98.2%; sequence variability was concentrated within CDS or between rDNA fragments (where indels were common). No phylogenetic reconstruction supported monophyly of Eimeria species infecting turkeys; Eimeria dispersa may have arisen

  9. Food Grade Pimenta Leaf Essential Oil Reduces the Attachment of Salmonella enterica Heidelberg (2011 Ground Turkey Outbreak Isolate on to Turkey Skin

    Directory of Open Access Journals (Sweden)

    Divek V. T. Nair

    2017-11-01

    Full Text Available Salmonella attached to the poultry skin is a major source of carcass contamination during processing. Once attached to the poultry skin, it is difficult to detach and inactivate Salmonella by commonly used antimicrobial agents since the pathogen is entrapped deeply in the feather follicles and the crevices on the skin. Essential oils could be natural, safe, and effective alternatives to synthetic antimicrobial agents during commercial and organic processing setup. The present study evaluated the efficacy of pimenta (Pimenta officinalis Lindl. leaf essential oil (PEO, and its nanoemulsion in reducing Salmonella Heidelberg attachment on to turkey (Meleagris gallopavo skin during simulated scalding (65°C and chilling (4°C steps in poultry processing. A multidrug resistant S. Heidelberg isolate from the 2011 ground turkey outbreak in the United States was used in the study. Results showed that PEO and the nanoemulsion resulted in significant reduction of S. Heidelberg attachment on turkey skin. Turkey skin samples treated with 1.0% PEO for 5 min resulted in >2 log10 CFU/sq. inch reduction of S. Heidelberg at 65 and 4°C, respectively (n = 6; P < 0.05. Similarly, skin samples treated with 1.0% pimenta nanoemulsion (PNE for 5 min resulted in 1.5- and 1.8- log10 CFU/sq. inch reduction of S. Heidelberg at 65 and 4°C, respectively (n = 6; P < 0.05. In addition, PEO and PNE were effective in reducing S. Heidelberg on skin during short-term storage at 4 and 10°C (temperature abuse (n = 6; P < 0.05. No Salmonella was detected in the dipping solution containing 0.5 or 1.0% PEO or PNE, whereas a substantial population of the pathogen survived in the control dipping solution. The results were validated using scanning electron -, and confocal - microscopy techniques. PEO or PNE could be utilized as an effective antimicrobial agent to reduce S. Heidelberg attachment to turkey skin during poultry processing.

  10. Molecular decay of enamel matrix protein genes in turtles and other edentulous amniotes

    Directory of Open Access Journals (Sweden)

    Meredith Robert W

    2013-01-01

    Full Text Available Abstract Background Secondary edentulism (toothlessness has evolved on multiple occasions in amniotes including several mammalian lineages (pangolins, anteaters, baleen whales, birds, and turtles. All edentulous amniote clades have evolved from ancestors with enamel-capped teeth. Previous studies have documented the molecular decay of tooth-specific genes in edentulous mammals, all of which lost their teeth in the Cenozoic, and birds, which lost their teeth in the Cretaceous. By contrast with mammals and birds, tooth loss in turtles occurred in the Jurassic (201.6-145.5 Ma, providing an extended time window for tooth gene degradation in this clade. The release of the painted turtle and Chinese softshell turtle genomes provides an opportunity to recover the decayed remains of tooth-specific genes in Testudines. Results We queried available genomes of Testudines (Chrysemys picta [painted turtle], Pelodiscus sinensis [Chinese softshell turtle], Aves (Anas platyrhynchos [duck], Gallus gallus [chicken], Meleagris gallopavo [turkey], Melopsittacus undulatus [budgerigar], Taeniopygia guttata [zebra finch], and enamelless mammals (Orycteropus afer [aardvark], Choloepus hoffmanni [Hoffmann’s two-toed sloth], Dasypus novemcinctus [nine-banded armadillo] for remnants of three enamel matrix protein (EMP genes with putative enamel-specific functions. Remnants of the AMBN and ENAM genes were recovered in Chrysemys and retain their original synteny. Remnants of AMEL were recovered in both testudines, although there are no shared frameshifts. We also show that there are inactivated copies of AMBN, AMEL and ENAM in representatives of divergent avian lineages including Galloanserae, Passeriformes, and Psittaciformes, and that there are shared frameshift mutations in all three genes that predate the basal split in Neognathae. Among enamelless mammals, all three EMP genes exhibit inactivating mutations in Orycteropus and Choloepus. Conclusions Our results

  11. Response of Turkey Muscle Satellite Cells to Thermal Challenge. II. Transcriptome Effects in Differentiating Cells

    Directory of Open Access Journals (Sweden)

    Kent M. Reed

    2017-11-01

    Full Text Available Background: Exposure of poultry to extreme temperatures during the critical period of post-hatch growth can seriously affect muscle development and thus compromise subsequent meat quality. This study was designed to characterize transcriptional changes induced in turkey muscle satellite cells by thermal challenge during differentiation. Our goal is to better define how thermal stress alters breast muscle ultrastructure and subsequent development.Results: Skeletal muscle satellite cells previously isolated from the Pectoralis major muscle of 7-wk-old male turkeys (Meleagris gallopavo from two breeding lines: the F-line (16 wk body weight-selected and RBC2 (randombred control line were used in this study. Cultured cells were induced to differentiate at 38°C (control or thermal challenge temperatures of 33 or 43°C. After 48 h of differentiation, cells were harvested and total RNA was isolated for RNAseq analysis. Analysis of 39.9 Gb of sequence found 89% mapped to the turkey genome (UMD5.0, annotation 101 with average expression of 18,917 genes per library. In the cultured satellite cells, slow/cardiac muscle isoforms are generally present in greater abundance than fast skeletal isoforms. Statistically significant differences in gene expression were observed among treatments and between turkey lines, with a greater number of genes affected in the F-line cells following cold treatment whereas more differentially expressed (DE genes were observed in the RBC2 cells following heat treatment. Many of the most significant pathways involved signaling, consistent with ongoing cellular differentiation. Regulation of Ca2+ homeostasis appears to be significantly affected by temperature treatment, particularly cold treatment.Conclusions: Satellite cell differentiation is directly influenced by temperature at the level of gene transcription with greater effects attributed to selection for fast growth. At lower temperature, muscle-associated genes in the

  12. Population status, feeding ecology and activity pattern of helmeted ...

    African Journals Online (AJOL)

    tedi

    2013-01-07

    Jan 7, 2013 ... 2Department of Biology, Addis Ababa University, P. O. Box 1176, Addis Ababa, Ethiopia. Accepted 7 ... fowl (Numida meleagris) in Abijata-Shalla Lakes National Park. Data were ..... Cambridge University Press, London.

  13. Browse Title Index

    African Journals Online (AJOL)

    Items 151 - 200 of 437 ... ... Stimulates Gluconeogenesis In Hypoproteinemic Albino Rat ... Oropharynx and Tongue of the Guinea Fowl (Numida Meleagris) ... Vol 7, No 3 (2010), Growth and reproductive performances of weaner pigs fed maize ...

  14. Indication of a species in an extinction vortex: The ocellated turkey on the Yucatan peninsula, Mexico

    NARCIS (Netherlands)

    Kampichler, C.; Calmé, S.; Weissenberger, H.; Arriaga-Weiss, S.

    2010-01-01

    The ocellated turkey Meleagris ocellata (OT) is a large, unmistakable endemic bird of the Yucatan peninsula. The species has suffered a considerable loss of distributional area as well as local abundance between 1980 and 2000 and is classified as endangered according to Mexican norms. We applied

  15. Ostrich: Journal of African Ornithology - Vol 75, No 4 (2004)

    African Journals Online (AJOL)

    Fowl play: identification and management of hybridisation between wild and domestic Helmeted Guineafowl (Numida meleagris) in South Africa · EMAIL FULL TEXT EMAIL FULL TEXT · DOWNLOAD FULL TEXT DOWNLOAD FULL TEXT. Andrew L Walker, Rauri CK Bowie, Charles S Ratcliffe, Timothy M Crowe, 195-198.

  16. Bulletin of Animal Health and Production in Africa - Vol 54, No 4 (2006)

    African Journals Online (AJOL)

    Post-brooding management of local guinea fowls (Numida meleagris) · EMAIL FULL TEXT EMAIL FULL TEXT · DOWNLOAD FULL TEXT DOWNLOAD FULL TEXT. HK Dei, P Dari, EO Otchere, KT Djang-Fordjour, A Donkoh, F Nelson, K Boa-Amposem, I Adam, 270-276. http://dx.doi.org/10.4314/bahpa.v54i4.32777 ...

  17. Author Details

    African Journals Online (AJOL)

    Meduna, A J. Vol 5 (2007) - Articles Effect of disturbance on laying pattern and hatchability of feral helmet guinea fowl (numida meleagris galleata pallas) egg. Abstract. ISSN: 1596-972X. AJOL African Journals Online. HOW TO USE AJOL... for Researchers · for Librarians · for Authors · FAQ's · More about AJOL · AJOL's ...

  18. Author Details

    African Journals Online (AJOL)

    Haematological evaluation of broilers administered probiotics (lactobacillus bulgaricus) Abstract · Vol 12, No 1 (2014) - Articles The growth performance and nutrient digestibility of wild indigenous guinea fowl keets (Numida meleagris galeata) fed varying levels of roasted Senna occidentalis seeds under intensive system.

  19. Turkey habitat use and nesting characteristics in ponderosa pine

    Science.gov (United States)

    Mark A. Rumble; Stanley H. Anderson

    1987-01-01

    Turkeys (Meleagris gallapovo) selected nest sites that provided good horizontal concealment. Rock or rock outcrops were selected most frequently for nest concealment on first-nest attempts. Renest attempts showed a selection preference for shrubs as nest cover; most of these were located in meadows. Nesting success doubled for renests versus first...

  20. Influenza Aviária: Uma Revisão dos Últimos Dez Anos

    Directory of Open Access Journals (Sweden)

    Martins NRS

    2001-01-01

    Full Text Available A influenza aviária é doença exótica no Brasil. O sistema de vigilância implementado pelo Programa Nacional de Sanidade Avícola (PNSA mantém monitoração permanente das aves das principais espécies domésticas, tanto do material genético importado para a indústria avícola, por exemplo, da espécie das galinhas (Gallus gallus formadomestica, perus (Meleagris gallopavo formadomestica, codornas (Coturnix coturnix japonica, patos (Anas, primários (elite, bisavós e avós para postura ou corte, como aves de espécies de exploração mais recente, exóticas, por exemplo avestruzes (Struthio camelus ou nativas, por exemplo emas (Rhea americana. Os plantéis de reprodutores em produção são também acompanhados por amostragens periódicas, conforme previsto no PNSA, além da monitoração das respostas aos programas de vacinação, por exemplo, contra bronquite infecciosa e doença infecciosa bursal. O PNSA estabelece as normas de atuação para o controle e erradicação da doença de Newcastle (ND e Influenza Aviária (AI (Projeto de Vigilância, 2001, a saber: I - Notificação de focos da doença (e confirmação laboratorial no LARA-Campinas; II - Assistência a focos; III - Medidas de desinfecção; IV - Sacrifício sanitário; V - Vazio sanitário; VI - Vacinação dos plantéis ou esquemas emergenciais; VII - Controle e fiscalização dos animais susceptíveis; VIII - Outras medidas sanitárias; A vigilância e atenção ao foco exige o diagnóstico laboratorial e diferencial de AI e ND, que segue as normas do PNSA, conforme o sumário abaixo: 1- Interdição e coleta de materiais para exame laboratorial oficial; 2- Registro das aves: espécie(s, categoria(s, número(s, manutenção de aves; utensílios e produtos no local; proibição de trânsito de e para a(s propriedade(s em um raio de 10 km; controle de todos os animais e materiais possíveis fontes de propagação; desinfecção de vias de entradas e saídas à(s propriedade

  1. Martini & Rossi – Madrid

    Directory of Open Access Journals (Sweden)

    de Ferrater Ramoneda, Jaime

    1965-04-01

    Full Text Available In the construction of this set of buildings, for a wine producing firm, the strictly functional needs were naturally the paramount design criterion. All the various stages in the manufacture of the well known liqueurs and aperitif drinks marketed by this firm had to be studied in detail, to provide the best possible housing accommodation. A detailed pattern of all the requirements, including communications, sequence of operations, and possible future developments, was the initial step from which the architects proceeded, in their progressive elaboration of the final design. The interplay of volumes and planes is a direct consequence of the essential purpose of the buildings. The bottling nave consists of a series of parabolic arches and portal frames which support, by means of adjustable tie members, many of the stored materials. This is an original and highly functional design, into which a great deal of care and thought has been put.En el planeamiento y construcción de cada una de las edificaciones que componen este establecimiento enológico, se siguió un criterio preponderantemente objetivo y funcional; para cada función sucesiva —del tratamiento de vinos para la obtención de aperitivos y licores— fue estudiado y diseñado el albergue adecuado, y estudiadas sus relaciones con las otras funciones. Del organigrama dimensionado se derivó directamente la, planta general y la composición de volúmenes; y de ello surgió la variedad y dinamisma que las mismas presentan. La nave de embotellado está constituida por una serie de pórticos—arcos parabólicos—, de los que queda suspendido, mediante tensores reglables, el almacén. En resumen, el conjunto construido destaca, por su originalidad y elegante sencillez.

  2. The conservation status of the Saldanha-Langebaan lizard fauna

    Directory of Open Access Journals (Sweden)

    I.G. Cordes

    1996-08-01

    Full Text Available The conservation status of relic melanistic lizard species occurring in the Saldanha- Langebaan area has been investigated. A contact zone between one melanistic form and a closely related non-melanistic form has been examined in detail. Apart from melanis- tic populations of the girdled lizards, Cordylus niger and C. polywnus, a melanistic morphotype of the Cape legless skink, Acontias meleagris meleagris also occurs in the area. The taxonomic status of this morphotype needs to be investigated. At Mauritz Bay, north of Saldanha, the ranges of C niger and the non-melanistic C cordylus are in contact over a distance of approximately 240 m, with maximum overlap of 70 m. The melanistic populations of C. polyz.onus and A. m. meleagris have relatively large ranges in the Saldanha-Langebaan area and are not threatened by urban development. The C niger population, however, is fragmented into several subpopulations, and those in the Saldanha area, including the contact zone, will be affected if urban development is allowed to continue in the area. As relic populations of other cool-adapted, melanistic invertebrate and lower vertebrate species may also occur in the area, the key areas demarcated by C. niger should be preserved.

  3. Floating nurseries? Scyphozoan jellyfish, their food and their rich symbiotic fauna in a tropical estuary

    Directory of Open Access Journals (Sweden)

    José M. Riascos

    2018-06-01

    Full Text Available Background The anthropogenic modification of trophic pathways is seemingly prompting the increase of jellyfish populations at the expense of planktivorous fishes. However, gross generalizations are often made because the most basic aspects of trophic ecology and the diverse interactions of jellyfish with fishes remain poorly described. Here we inquire on the dynamics of food consumption of the medusoid stage of the scyphozoan jellyfish Stomolophus meleagris and characterize the traits and diversity of its symbiotic community. Methods S. meleagris and their associated fauna were sampled in surface waters between November 2015 and April 2017 in Málaga Bay, an estuarine system at the Colombian Pacific. Stomach contents of medusae were examined and changes in prey composition and abundance over time analysed using a multivariate approach. The associated fauna was identified and the relationship between the size of medusae and the size those organisms tested using least-square fitting procedures. Results The presence of S. meleagris medusa in surface waters was seasonal. The gut contents analysis revealed that algae, copepods and fish early life stages were the more abundant items, and PERMANOVA analysis showed that the diet differed within the seasons (P(perm = 0.001 but not between seasons (P(perm = 0.134. The majority of the collected medusae (50.4% were associated with individuals of 11 symbiotic species, 95.3% of them fishes, 3.1% crustaceans and 1.6% molluscs. Therefore, this study reports 10 previously unknown associations. The bell diameter of S. meleagris was positively related to the body sizes of their symbionts. However, a stronger fit was observed when the size relationship between S. meleagris and the fish Hemicaranx zelotes was modelled. Discussion The occurrence of S. meleagris was highly seasonal, and the observed patterns of mean body size through the seasons suggested the arrival of adult medusae to the estuary from adjacent

  4. Floating nurseries? Scyphozoan jellyfish, their food and their rich symbiotic fauna in a tropical estuary

    Science.gov (United States)

    Aguirre, Willington; Hopfe, Charlotte; Morales, Diego; Navarrete, Ángela; Tavera, José

    2018-01-01

    Background The anthropogenic modification of trophic pathways is seemingly prompting the increase of jellyfish populations at the expense of planktivorous fishes. However, gross generalizations are often made because the most basic aspects of trophic ecology and the diverse interactions of jellyfish with fishes remain poorly described. Here we inquire on the dynamics of food consumption of the medusoid stage of the scyphozoan jellyfish Stomolophus meleagris and characterize the traits and diversity of its symbiotic community. Methods S. meleagris and their associated fauna were sampled in surface waters between November 2015 and April 2017 in Málaga Bay, an estuarine system at the Colombian Pacific. Stomach contents of medusae were examined and changes in prey composition and abundance over time analysed using a multivariate approach. The associated fauna was identified and the relationship between the size of medusae and the size those organisms tested using least-square fitting procedures. Results The presence of S. meleagris medusa in surface waters was seasonal. The gut contents analysis revealed that algae, copepods and fish early life stages were the more abundant items, and PERMANOVA analysis showed that the diet differed within the seasons (P(perm) = 0.001) but not between seasons (P(perm) = 0.134). The majority of the collected medusae (50.4%) were associated with individuals of 11 symbiotic species, 95.3% of them fishes, 3.1% crustaceans and 1.6% molluscs. Therefore, this study reports 10 previously unknown associations. The bell diameter of S. meleagris was positively related to the body sizes of their symbionts. However, a stronger fit was observed when the size relationship between S. meleagris and the fish Hemicaranx zelotes was modelled. Discussion The occurrence of S. meleagris was highly seasonal, and the observed patterns of mean body size through the seasons suggested the arrival of adult medusae to the estuary from adjacent waters. The diet

  5. Floating nurseries? Scyphozoan jellyfish, their food and their rich symbiotic fauna in a tropical estuary.

    Science.gov (United States)

    Riascos, José M; Aguirre, Willington; Hopfe, Charlotte; Morales, Diego; Navarrete, Ángela; Tavera, José

    2018-01-01

    The anthropogenic modification of trophic pathways is seemingly prompting the increase of jellyfish populations at the expense of planktivorous fishes. However, gross generalizations are often made because the most basic aspects of trophic ecology and the diverse interactions of jellyfish with fishes remain poorly described. Here we inquire on the dynamics of food consumption of the medusoid stage of the scyphozoan jellyfish Stomolophus meleagris and characterize the traits and diversity of its symbiotic community. S. meleagris and their associated fauna were sampled in surface waters between November 2015 and April 2017 in Málaga Bay, an estuarine system at the Colombian Pacific. Stomach contents of medusae were examined and changes in prey composition and abundance over time analysed using a multivariate approach. The associated fauna was identified and the relationship between the size of medusae and the size those organisms tested using least-square fitting procedures. The presence of S. meleagris medusa in surface waters was seasonal. The gut contents analysis revealed that algae, copepods and fish early life stages were the more abundant items, and PERMANOVA analysis showed that the diet differed within the seasons ( P (perm)  = 0.001) but not between seasons ( P (perm)  = 0.134). The majority of the collected medusae (50.4%) were associated with individuals of 11 symbiotic species, 95.3% of them fishes, 3.1% crustaceans and 1.6% molluscs. Therefore, this study reports 10 previously unknown associations. The bell diameter of S. meleagris was positively related to the body sizes of their symbionts. However, a stronger fit was observed when the size relationship between S. meleagris and the fish Hemicaranx zelotes was modelled. The occurrence of S. meleagris was highly seasonal, and the observed patterns of mean body size through the seasons suggested the arrival of adult medusae to the estuary from adjacent waters. The diet of S. meleagris in the study

  6. [Susceptibility of birds other than chickens to infectious laryngotracheitis].

    Science.gov (United States)

    Hilbink, F W

    1985-06-01

    Susceptibility to infectious laryngotracheitis virus was studied in peafowl (Pavo cristatus), various species of pheasant (Phasianus colchicus, Lophura swinhoeii, Lophophorus impejanus), guinea-fowl (Numida meleagris), canaries (Serinus canaria), budgerigars (Melopsittacus undulatus) and Japanese quail (Coturnix coturnic japonica). Apart from clinical observations, experiments were evaluated in terms of histopathology, immunofluorescence, serology and recovery of virus. Only peafowl and pheasants were found to be susceptible, pheasants responding more strongly than chickens to ocular vaccination and intratracheal inoculation. The other species were found to be refractory.

  7. New records of lice (Insecta: Phthiraptera) from domestic and ornamental birds from Chile

    OpenAIRE

    González-Acuña, D; Lara, J; Cicchino, A

    2009-01-01

    Por medio del examen del plumaje de aves domésticas y ornamentales en Chile se reconfirma la presencia de los phthiraptera Menopon gallinae, Goniodes gigas, Goniocotes gallinae y Menacanthus stramineus en la gallina doméstica, Gallus gallus domesticus y se registra además por primera vez en el país la especie Menacanthus pallidulus. En aves ornamentales se registran en pavo, Meleagris gallipavo, la especie M. stramineus, en pavo real, Pavo cristatus las especies Goniodes pavonis y Amrysidea m...

  8. Nuevos registros de piojos (Insecta: Phthiraptera) en aves domésticas y ornamentales en Chile New records of lice (Insecta: Phthiraptera) from domestic and ornamental birds from Chile

    OpenAIRE

    D González-Acuña; J Lara; A Cicchino

    2009-01-01

    Por medio del examen del plumaje de aves domésticas y ornamentales en Chile se reconfirma la presencia de los phthiraptera Menopon gallinae, Goniodes gigas, Goniocotes gallinae y Menacanthus stramineus en la gallina doméstica, Gallus gallus domesticus y se registra además por primera vez en el país la especie Menacanthus pallidulus. En aves ornamentales se registran en pavo, Meleagris gallipavo, la especie M. stramineus, en pavo real, Pavo cristatus las especies Goniodes pavonis y Amrysidea m...

  9. Cubozoa e Scyphozoa (Cnidaria: Medusozoa de águas costeiras do Brasil Cubozoa and Scyphozoa (Cnidaria: Medusozoa from Brazilian coastal waters

    Directory of Open Access Journals (Sweden)

    André C. Morandini

    2005-09-01

    Full Text Available As espécies de Cubozoa e Scyphozoa costeiras que ocorrem no Brasil são descritas, com base em espécimes de coleções de museus e exemplares recém-coletados. Chaves de identificação e um glossário também são apresentados. As espécies descritas são: Aurelia sp.; Cassiopea xamachana Bigelow, 1892; Chiropsalmus quadrumanus (Müller, 1859; Chrysaora lactea Eschscholtz, 1829; Drymonema dalmatinum Haeckel, 1880; Linuche unguiculata (Swartz, 1788; Lychnorhiza lucerna Haeckel, 1880; Nausithoe aurea Silveira & Morandini, 1997; Phyllorhiza punctata von Lendenfeld, 1884; Stomolophus meleagris Agassiz, 1862; Tamoya haplonema Müller, 1859 e Tripedalia cystophora Conant, 1897.Coastal species of Cubozoa and Scyphozoa occurring in Brazil are described, based on museum specimens and recently collected ones. Identification keys and a glossary are also presented. The listed species are: Aurelia sp.; Cassiopea xamachana Bigelow, 1892; Chiropsalmus quadrumanus (Müller, 1859; Chrysaora lactea Eschscholtz, 1829; Drymonema dalmatinum Haeckel, 1880; Linuche unguiculata (Swartz, 1788; Lychnorhiza lucerna Haeckel, 1880; Nausithoe aurea Silveira & Morandini, 1997; Phyllorhiza punctata von Lendenfeld, 1884; Stomolophus meleagris Agassiz, 1862; Tamoya haplonema Müller, 1859; and Tripedalia cystophora Conant, 1897.

  10. Clearing of suspensions of Micrococcus lysodeikticus catalysed by lysozymes from hen, goose, and turkey egg whites, human milk, and phage T4. Assessment of potential as signal generators for homogeneous enzyme immunoassays for urinary steroids.

    Science.gov (United States)

    Cooke, Delwyn G; Blackwell, Leonard F

    2007-01-01

    Lysozymes (3.2.1.17) from goose (Anser anser) egg white, turkey (Melagris gallopavo) egg white, phage T4 and human milk were compared with hen egg white lysozyme in their ability to clear a suspension of Micrococcus lysodeikticus. All of the lysozymes, except hen egg white lysozyme, catalysed the clearing of the Micrococcus lysodeikticus suspension in a biphasic fashion. Compared to hen egg white lysozyme, the total absorbance or transmission change over 5 and 20 minutes was less in all cases, except for human lysozyme. Human lysozyme was, therefore, a potential alternative, more rapid signal generator for the measurement of urinary estrone glucuronide excretion rates because of its structural similarity to hen egg white lysozyme. The apparent K(M) values for hen egg white lysozyme increased with the enzyme concentration.

  11. A pyranose dehydrogenase-based biosensor for kinetic analysis of enzymatic hydrolysis of cellulose by cellulases

    DEFF Research Database (Denmark)

    Cruys-Bagger, Nicolaj; Badino, Silke Flindt; Tokin, Radina Naytchova

    2014-01-01

    A novel electrochemical enzyme biosensor was developed for real-time detection of cellulase activity when acting on their natural insoluble substrate, cellulose. The enzyme biosensor was constructed with pyranose dehydrongease (PDH) from Agaricus meleagris that was immobilized on the surface......-biosensor was shown to be anomer unspecific and it can therefore be used in kinetic studies over broad time-scales of both retaining- and inverting cellulases (in addition to enzyme cocktails). The biosensor was used for real-time measurements of the activity of the inverting cellobiohydrolase Cel6A from Hypocrea...... equation for processive cellulases, and it was found that the turnover for HjCel6A at saturating substrate concentration (i.e. maximal apparent specific activity) was similar (0.39–0.40 s−1) for the two substrates. Conversely, the substrate load at half-saturation was much lower for BMCC compared to Avicel...

  12. Fish corallivory on a pocilloporid reef and experimental coral responses to predation

    Science.gov (United States)

    Palacios, M. M.; Muñoz, C. G.; Zapata, F. A.

    2014-09-01

    This study examined the effects of the Guineafowl pufferfish ( Arothron meleagris), a major corallivore in the Eastern Pacific, on pocilloporid corals on a reef at Gorgona Island, Colombia. Pufferfish occurred at a density of 171.2 individuals ha-1 and fed at a rate of 1.8 bites min-1, which produced a standing bite density of 366.2 bites m-2. We estimate that approximately 15.6 % of the annual pocilloporid carbonate production is removed by the pufferfish population. Examination of the predation effect on individual pocilloporid colonies revealed that although nubbins exposed to corallivory had lower linear growth, they gained similar weight and became thicker than those protected from it. Additionally, colonies with simulated predation injuries (on up to 75 % of branch tips) healed successfully and maintained growth rates similar to those of uninjured colonies. Despite the high corallivore pressure exerted by pufferfish on this reef, we conclude that they have a low destructive impact on Pocillopora colonies as corals can maintain their carbonate production rate while effectively recovering from partial predation. Due to its influence on colony morphology, pufferfish predation may increase environmentally induced morphological variability in Pocillopora.

  13. Confirmed organophosphorus and carbamate pesticide poisonings in South African wildlife (2009–2014

    Directory of Open Access Journals (Sweden)

    Christo J. Botha

    2015-12-01

    Full Text Available During a six-year period (from January 2009 to December 2014, specimens collected from 344 cases of suspected organophosphorus and carbamate pesticide poisonings in wildlife, including birds, were submitted to the Toxicology Laboratory (ARC-OVI for analysis. A positive diagnosis was made in 135 (39% of these cases. The majority of cases were from birds, which included Cape vultures (Gyps coprotheres and African white-backed vultures (Gyps africanus and bateleur eagles (Terathopius ecaudatus. In one incident 49 vultures were killed when a farmer intentionally laced carcasses with carbofuran in an attempt to control jackal predation. There were 22 incidents of poisoning in helmeted guineafowl (Numida meleagris. On nine different occasions blue cranes (Anthropoides paradiseus were poisoned, in one incident 14 birds were reported to have been killed. Over the period of investigation, there were 20 cases of poisoning involving mammalian species, the majority being vervet monkeys (Chlorocebus pygerythrus. The carbamate pesticides were responsible for 57 incidents of poisoning. Aldicarb, carbofuran and methomyl were detected in 26, 18 and 12 cases respectively. The majority of organophosphorus pesticide poisonings were caused by diazinon (n = 19, monocrotophos (n = 13 and methamidophos (n = 10.

  14. Morphometric study of the avian adrenal gland.

    Science.gov (United States)

    Aire, T A

    1980-01-01

    The interrenal and medullary cords as well as the blood vessels and connective tissue proportions in the adrenal glands of the male Nigerian fowl (Gallus domesticus) and guinea-fowl (Numida meleagris) were studied by microstereological techniques. Laying domestic fowl of the Rhode Island Red breed were entirely defeathered and maintained in a hot, humid pen for a period of three months, after which the adrenal glands were also studied microstereologically. Interrenal cord width was also measured in all the birds studied. The interrenal cords of the subscapular zone were consistently wider than those cords in the inner zone of the adrenal glands. This clearly suggested morphological zoning. The proportion of interrenal tissue was significantly greater in the guinea-fowl than in the Nigerian fowl, but the medullary tissue and the blood vessels and connective tissue were not significantly different. Interrenal hypoplasia or medullary hyperplasia occurred in the defeathered Rhode Island Red fowl as compared to the control birds of the same breed and sex. The significance of these findings is discussed. PMID:7440402

  15. Yolk formation in some Charadriiform birds

    Science.gov (United States)

    Roudybush, T.E.; Grau, C.R.; Petersen, M.R.; Ainley, D.G.; Hirsch, K.V.; Gilman, A.P.; Patten, S.M.

    1979-01-01

    By counting and measuring the major ova of breeding birds at autopsy and combining these data with time intervals between ovipositions, rough estimates have been made of the time required to form yolk in some non-captive birds (King 1973). Direct studies have been made in domestic fowl (Gallus gallus var. domesticus; Gilbert 1972), turkeys (Meleagris galloparvo; Bacon and Cherms 1968), and Common quail (Coturnix coturnix; Bacon and Koontz 1971), by feeding the birds a capsule containing dye each day, and counting dye rings in the yolks after the eggs have been hardcooked. Recently developed methods of fixing and staining eggs have revealed differences in yolk deposited during day and night, thus permitting another estimation of the number of days during which yolk was deposited, and without direct contact with the female (Grau 1976). In eggs from chickens and quail that have been fed dyes, yolk that stained darkly with dichromate was shown to be deposited during the active daytime feeding periods, while pale-staining yolk was deposited during the night. Thus, pairs of light and dark rings, which together take a day to be deposited, may be counted to estimate time of yolk formation.In the present study we have applied the yolk ring method of estimating the number of days during which the bulk of the yolk is deposited around the central white core (Grau 1976) to the eggs of some shorebirds, gulls, terns and alcids.

  16. Distribution of endemic and introduced tick species in Free State Province, South Africa

    Directory of Open Access Journals (Sweden)

    Ivan G. Horak

    2015-06-01

    Full Text Available The distributions of endemic tick vector species as well as the presence of species not endemic to Free State Province, South Africa, were determined during surveys or opportunistic collections from livestock, wildlife and vegetation. Amongst endemic ticks, the presence of Rhipicephalus appendiculatus was confirmed in the north of the province, whilst Rhipicephalus decoloratus was collected at 31 localities mostly in the centre and east, and Ixodes rubicundus at 11 localities in the south, south-west and centre of the province. Amongst the non-endemic species adult Amblyomma hebraeum were collected from white rhinoceroses (Ceratotherium simum on four privately owned farms, whilst the adults of Rhipicephalus microplus were collected from cattle and a larva from vegetation at four localities in the east of the province. The collection of Rhipicephalus evertsi mimeticus from a sheep in the west of the province is the second record of its presence in the Free State, whereas the presence of Haemaphysalis silacea on helmeted guineafowl (Numida meleagris and vegetation in the centre of the province represents a first record for this species in the Free State. The first collection of the argasid tick, Ornithodoros savignyi, in the Free State was made from a domestic cow and from soil in the west of the province. The localities at which the ticks were collected have been plotted and the ticks’ role in the transmission or cause of disease in domestic livestock and wildlife is discussed.

  17. Polymerase chain reaction assay for verifying the labeling of meat and commercial meat products from game birds targeting specific sequences from the mitochondrial D-loop region.

    Science.gov (United States)

    Rojas, M; González, I; Pavón, M A; Pegels, N; Hernández, P E; García, T; Martín, R

    2010-05-01

    A PCR assay was developed for the identification of meats and commercial meat products from quail (Coturnix coturnix), pheasant (Phasianus colchicus), partridge (Alectoris spp.), guinea fowl (Numida meleagris), pigeon (Columba spp.), Eurasian woodcock (Scolopax rusticola), and song thrush (Turdus philomelos) based on oligonucleotide primers targeting specific sequences from the mitochondrial D-loop region. The primers designed generated specific fragments of 96, 100, 104, 106, 147, 127, and 154 bp in length for quail, pheasant, partridge, guinea fowl, pigeon, Eurasian woodcock, and song thrush tissues, respectively. The specificity of each primer pair was tested against DNA from various game and domestic species. In this work, satisfactory amplification was accomplished in the analysis of experimentally pasteurized (72 degrees C for 30 min) and sterilized (121 degrees C for 20 min) meats, as well as in commercial meat products from the target species. The technique was also applied to raw and sterilized muscular binary mixtures, with a detection limit of 0.1% (wt/wt) for each of the targeted species. The proposed PCR assay represents a rapid and straightforward method for the detection of possible mislabeling in game bird meat products.

  18. The islands are different: human perceptions of game species in Hawaii.

    Science.gov (United States)

    Lohr, Cheryl A; Lepczyk, Christopher A; Johnson, Edwin D

    2014-10-01

    Hawaii's game animals are all non-native species, which provokes human-wildlife conflict among stakeholders. The management of human-wildlife conflict in Hawaii is further complicated by the discrete nature of island communities. Our goal was to understand the desires and perceived values or impacts of game held by residents of Hawaii regarding six game species [pigs (Sus scrofa), goats (Capra hircus), mouflon (Ovis musimon), axis deer (Axis axis), turkeys (Melagris gallopavo), and doves (Geopelia striata)]. We measured the desired abundance of game on the six main Hawaiian Islands using the potential for conflict index and identified explanatory variables for those desires via recursive partitioning. In 2011 we surveyed 5,407 residents (2,360 random residents and 3,047 pre-identified stakeholders). Overall 54.5 and 27.6 % of the emailed and mailed surveys were returned (n = 1,510). A non-respondent survey revealed that respondents and non-respondents had similar interest in wildlife, and a similar education level. The desired abundance of game differed significantly among stakeholders, species, and islands. The desired abundance scores were higher for axis deer, mouflon, and turkeys compared to pigs, goats or doves. Enjoyment at seeing game and the cultural value of game were widespread explanatory variables for desired abundance. Models for Lanai emphasized the economic value of game, whereas models for Maui identified the potential for game to contaminate soil and water. Models for Oahu and Kauai revealed concern for human health and safety. Given our findings we recommend managers design separate management plans for each island taking into consideration the values of residents.

  19. Osmotic tolerance of avian spermatozoa: Influence of time, temperature, cryoprotectant and membrane ion pump function on sperm viability

    Science.gov (United States)

    Blanco, J.M.; Long, J.A.; Gee, G.; Donoghue, A.M.; Wildt, D.E.

    2008-01-01

    Potential factors influencing sperm survival under hypertonic conditions were evaluated in the Sandhill crane (Grus canadensis) and turkey (Meleagridis gallopavo). Sperm osmotolerance (300-3000 mOsm/kg) was evaluated after: (1) equilibration times of 2, 10, 45 and 60 min at 4 ?C versus 21 ?C; (2) pre-equilibrating with dimethylacetamide (DMA) or dimethylsulfoxide (Me2SO) at either 4 ?C or 21 ?C; and (3) inhibition of the Na+/K+ and the Na+/H+ antiporter membrane ionic pumps. Sperm viability was assessed using the eosin-nigrosin live/dead stain. Species-specific differences occurred in response to hypertonic conditions with crane sperm remaining viable under extreme hypertonicity (3000 mOsm/kg), whereas turkey sperm viability was compromised with only slightly hypertonic (500 mOsm/kg) conditions. The timing of spermolysis under hypertonic conditions was also species-specific, with a shorter interval for turkey (2 min) than crane (10 min) sperm. Turkey sperm osmotolerance was slightly improved by lowering the incubation temperature from 21 to 4 ?C. Pre-equilibrating sperm with DMA reduced the incidence of hypertonic spermolysis only in the crane, at both room and refrigeration temperature. Inhibiting the Na+/K+ and the Na+/H+ antiporter membrane ion pumps did not impair resistance of crane and turkey spermatozoa to hypertonic stress; pump inhibition actually increased turkey sperm survival compared to control sperm. Results demonstrate marked species specificity in osmotolerance between crane and turkey sperm, as well as in the way temperature and time of exposure affect sperm survival under hypertonic conditions. Differences are independent of the role of osmotic pumps in these species.

  20. The Islands Are Different: Human Perceptions of Game Species in Hawaii

    Science.gov (United States)

    Lohr, Cheryl A.; Lepczyk, Christopher A.; Johnson, Edwin D.

    2014-10-01

    Hawaii's game animals are all non-native species, which provokes human-wildlife conflict among stakeholders. The management of human-wildlife conflict in Hawaii is further complicated by the discrete nature of island communities. Our goal was to understand the desires and perceived values or impacts of game held by residents of Hawaii regarding six game species [pigs ( Sus scrofa), goats ( Capra hircus), mouflon ( Ovis musimon), axis deer ( Axis axis), turkeys ( Melagris gallopavo), and doves ( Geopelia striata)]. We measured the desired abundance of game on the six main Hawaiian Islands using the potential for conflict index and identified explanatory variables for those desires via recursive partitioning. In 2011 we surveyed 5,407 residents (2,360 random residents and 3,047 pre-identified stakeholders). Overall 54.5 and 27.6 % of the emailed and mailed surveys were returned ( n = 1,510). A non-respondent survey revealed that respondents and non-respondents had similar interest in wildlife, and a similar education level. The desired abundance of game differed significantly among stakeholders, species, and islands. The desired abundance scores were higher for axis deer, mouflon, and turkeys compared to pigs, goats or doves. Enjoyment at seeing game and the cultural value of game were widespread explanatory variables for desired abundance. Models for Lanai emphasized the economic value of game, whereas models for Maui identified the potential for game to contaminate soil and water. Models for Oahu and Kauai revealed concern for human health and safety. Given our findings we recommend managers design separate management plans for each island taking into consideration the values of residents.

  1. Effect of dietary supplementation of herbal seeds on carcass traits of turkey poults

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    Darshana B. Bhaisare

    2014-11-01

    Full Text Available Aim: The present study was conducted to find the effect of four herbal seeds on carcass traits of turkey poults. Materials and Methods: A biological study using Nandanam turkey poults (Meleagris gallapavo for 8 weeks duration was carried out to evaluate the effect of phytobiotics-containing four herbal seeds influence on production performances like biweekly body weight and on carcass traits. 150 poults were randomly subjected to five dietary treatments in a completely randomized design with basal diet (T1, 0.5% (5 g/kg level of each seeds thyme (Thymus vulgaris (T2, fenugreek (Trigonella foenum graecum (T3, fennel (Foeniculum vulgare (T4 and cumin (Cuminum cyminum (T5. Carcass traits like blood loss, feather loss, dressed weight, New York dressed weight, ready to cook yield and cut-up parts yield were studied. Results: The body weight at 8th week was higher (p<0.05 in poults fed with thyme; whereas at 6th week, fennel and cumin fed birds had better (p<0.05 body weight. Inclusion of herbal seeds did not affect the blood loss, dressed weight and ready to cook yield but it significantly (p<0.05 affected the feathered loss, New York dressed weight and giblet percentages. Feeding of fenugreek has improved New York dressed weight of poults. Feeding of fennel had depressive (p<0.05 effect on liver and gizzard weights. All the four phytobiotic seeds in feed had significant (p<0.05 reduction in breast weight with a compensatory improvement in drumstick and neck weights. Conclusion: The present study revealed that supplementation of phytobiotic herbal seeds has resulted in numerical improvement of body weight of poults throughout the study period whereas these seeds had negative effect on the yield of breast, with increased proportion of drumstick and neck.

  2. Hosts, seasonality and geographic distribution of the South African tortoise tick, Amblyomma marmoreum

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    I.G. Horak

    2006-09-01

    Full Text Available The tortoise tick Amblyomma marmoreum was collected from large numbers of reptiles and other animals during the course of numerous surveys conducted in South Africa. A total of 1 229 ticks, of which 550 were adults, were recovered from 309 reptiles belonging to 13 species, with leopard tortoises, Geochelone pardalis being the most heavily infested. The 269 birds sampled harboured 4 901 larvae, 217 nymphs and no adult ticks, and the prevalence of infestation was greatest on hel meted guinea fowls, Numida meleagris. Only two larvae were recovered from 610 rodents, including 31 spring hares, Pedetes capensis, whereas 1 144 other small mammals yielded 1 835 immature ticks, of which 1 655 were collected from 623 scrub hares, Lepus saxatilis. The 213 carnivores examined harboured 2 459 ticks of which none were adult. A single adult tick and 6 684 larvae and 62 nymphs were recovered from 656 large herbivores, and a total of 4 081 immature ticks and three adults were collected from 1 543 domestic animals and 194 humans. Adult male and female A. marmoreum were most numerous on reptiles during January and February, and larvae during March. The largest numbers of larvae were present on domestic cattle and helmeted guineafowls in the Eastern Cape Province during March or April respectively, whereas larvae were most numerous on helmeted guineafowls, scrub hares and the vegetation in north-eastern Mpumalanga Province during May. In both provinces nymphs were most numerous between October and December. Amblyomma marmoreum appears to be most prevalent in the western regions of the Western and Eastern Cape and Free State provinces, and the north-eastern regions of the Northern Cape, KwaZulu- Natal, Mpumulanga and Limpopo provinces.

  3. Pyranose dehydrogenase ligand promiscuity: a generalized approach to simulate monosaccharide solvation, binding, and product formation.

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    Michael M H Graf

    2014-12-01

    Full Text Available The flavoenzyme pyranose dehydrogenase (PDH from the litter decomposing fungus Agaricus meleagris oxidizes many different carbohydrates occurring during lignin degradation. This promiscuous substrate specificity makes PDH a promising catalyst for bioelectrochemical applications. A generalized approach to simulate all 32 possible aldohexopyranoses in the course of one or a few molecular dynamics (MD simulations is reported. Free energy calculations according to the one-step perturbation (OSP method revealed the solvation free energies (ΔGsolv of all 32 aldohexopyranoses in water, which have not yet been reported in the literature. The free energy difference between β- and α-anomers (ΔGβ-α of all d-stereoisomers in water were compared to experimental values with a good agreement. Moreover, the free-energy differences (ΔG of the 32 stereoisomers bound to PDH in two different poses were calculated from MD simulations. The relative binding free energies (ΔΔGbind were calculated and, where available, compared to experimental values, approximated from Km values. The agreement was very good for one of the poses, in which the sugars are positioned in the active site for oxidation at C1 or C2. Distance analysis between hydrogens of the monosaccharide and the reactive N5-atom of the flavin adenine dinucleotide (FAD revealed that oxidation is possible at HC1 or HC2 for pose A, and at HC3 or HC4 for pose B. Experimentally detected oxidation products could be rationalized for the majority of monosaccharides by combining ΔΔGbind and a reweighted distance analysis. Furthermore, several oxidation products were predicted for sugars that have not yet been tested experimentally, directing further analyses. This study rationalizes the relationship between binding free energies and substrate promiscuity in PDH, providing novel insights for its applicability in bioelectrochemistry. The results suggest that a similar approach could be applied to study

  4. Fossil bird eggs from the Pliocene of Laetoli, Tanzania: Their taxonomic and paleoecological relationships

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    Harrison, Terry

    2005-04-01

    Recent paleontological investigations at the Pliocene site of Laetoli and at neighboring localities on the Eyasi Plateau of northern Tanzania have led to the recovery of a sizable collection of fossil bird eggs. The material comes from the Upper Laetolil Beds, dated at ˜3.6-3.8 Ma, and the Lower Laetolil Beds, dated at 3.8 Ma to older than 4.3 Ma. The preservation of relatively complete eggs (other than those of ratites) is an extremely rare occurrence in the fossil record, and Laetoli is the only locality in Africa that has produced such well-preserved eggs. Deposition of carbonatite air-fall tuffs led to the rapid burial of the eggs sub-aerially, and they were then preserved in paleosols that were geochemically conducive to their preservation. The collection of fossil eggs from Laetoli can be assigned to at least five different species of ground-nesting birds, including two or three species of francolins, a species of guineafowl, and a larger bird of uncertain taxonomic status about the size of a bustard. Most of the eggs can be assigned to a large species of Francolinus, similar in size to the extant F. afer and F. leucoscepus. A smaller species of francolin, about the size of Francolinus coqui or F. sephaena, is also represented, but is less common. A single egg may represent an even smaller species of francolin, about the size of Francolinus lathami or F. nahani, but its attribution to Francolinus is less certain. The evidence of at least two species of Francolinus at Laetoli indicates that francolins were already taxonomically diverse in East Africa by the mid-Pliocene. Three eggs are similar in their overall dimensions and morphology to the living Numida meleagris, the helmeted guineafowl. An avian community including at least one small species of francolin, a larger francolin, and a guineafowl (as well as ostriches and a vulture) implies that the paleoecology at Laetoli was likely to have been open woodland, bushland, savanna or grassland. However

  5. Clipperton Atoll (eastern Pacific): oceanography, geomorphology, reef-building coral ecology and biogeography

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    Glynn, P. W.; Veron, J. E. N.; Wellington, G. M.

    1996-06-01

    coralline algae are dominant (5 40% cover) from 0.5 to 7m. Spur and groove features, constructed of alternating frameworks of Pocillopora and Porites, and veneered with crustose coralline algae, are generally well developed around most atoll exposures. Although crustose coralline algae predominate in the breaker zone (with up to 100% cover), a prominent algal ridge is absent with only a slight buildup (ca. 10 cm) to seaward. Frequent grazing by the pufferfish Arothron meleagris results in the removal of large amounts of live tissue and skeleton from Porites lobata. Acanthaster planci is present, but rare. The grazing of large diadematid sea urchins, (2 species each of Diadema and Echinothrix) on dead corals cause extensive erosion in some areas. Large numbers of corals on the 15 18 m terrace had recently suffered partial ( P. lobata, 60 70% maximum of all colonies sampled) or total ( Pocillopora sp., 80% maximum) mortality. The lengths of regenerating knobs and the rates of linear skeletal growth in P. lobata, determined by sclerochronologic analysis, indicated a period of stress during 1987. Massive skeletal growth is significantly higher at intermediate (16 17 m) than shallow (6 8 m) depths with mean extension rates of 1.5 mm yr-1 in P. lobata and 1.4 mm yr-1 in P. minuta at intermediate depths. Skeletal growth in P. lobata was depressed during the 1987 El Nifio event at Clipperton. The branching coral Pocillopora sp. demonstrated high and similar skeletal growth rates at both shallow (25.4 mm yr-1) and intermediate (26.5 mm yr-1) depths. The presence of widely distributed Indo-Pacific zooxanthellate corals at Clipperton and the Revillagigedo Islands indicates that these NE Pacific Islands probably serve as a stepping stone for dispersal into the far eastern Pacific region.

  6. Annotated type catalogue of the Bulimulidae (Mollusca, Gastropoda, Orthalicoidea in the Natural History Museum, London

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    Abraham Breure

    2014-03-01

    Full Text Available The type status is described of 404 taxa classified within the family Bulimulidae (superfamily Orthalicoidea and kept in the London museum. Lectotypes are designated for Bulimus aurifluus Pfeiffer, 1857; Otostomus bartletti H. Adams, 1867; Helix cactorum d’Orbigny, 1835; Bulimus caliginosus Reeve, 1849; Bulimus chemnitzioides Forbes, 1850; Bulimus cinereus Reeve, 1849; Helix cora d’Orbigny, 1835; Bulimus fallax Pfeiffer, 1853; Bulimus felix Pfeiffer, 1862; Bulimus fontainii d’Orbigny, 1838; Bulimus fourmiersi d’Orbigny, 1837; Bulimus (Mesembrinus gealei H. Adams, 1867; Bulimus gruneri Pfeiffer, 1846; Bulimus humboldtii Reeve, 1849; Helix hygrohylaea d’Orbigny, 1835; Bulimus jussieui Pfeiffer, 1846; Bulimulus (Drymaeus binominis lascellianus E.A. Smith, 1895; Helix lichnorum d’Orbigny, 1835; Bulimulus (Drymaeus lucidus da Costa, 1898; Bulimus luridus Pfeiffer, 1863; Bulimus meleagris Pfeiffer, 1853; Bulimus monachus Pfeiffer, 1857; Bulimus montagnei d’Orbigny, 1837; Helix montivaga d’Orbigny, 1835; Bulimus muliebris Reeve, 1849; Bulimus nigrofasciatus Pfeiffer in Philippi 1846; Bulimus nitelinus Reeve, 1849; Helix oreades d’Orbigny, 1835; Helix polymorpha d’Orbigny, 1835; Bulimus praetextus Reeve, 1849; Bulinus proteus Broderip, 1832; Bulimus rusticellus Morelet, 1860; Helix sporadica d’Orbigny, 1835; Bulimus sulphureus Pfeiffer, 1857; Helix thamnoica var. marmorata d’Orbigny, 1835; Bulinus translucens Broderip in Broderip and Sowerby I 1832; Helix trichoda d’Orbigny, 1835; Bulinus ustulatus Sowerby I, 1833; Bulimus voithianus Pfeiffer, 1847; Bulimus yungasensis d’Orbigny, 1837.The type status of the following taxa is changed to lectotype in accordance with Art. 74.6 ICZN: Bulimulus (Drymaeus caucaensis da Costa, 1898; Drymaeus exoticus da Costa, 1901; Bulimulus (Drymaeus hidalgoi da Costa, 1898; Bulimulus (Drymaeus interruptus Preston, 1909; Bulimulus (Drymaeus inusitatus Fulton, 1900; Bulimulus latecolumellaris