WorldWideScience

Sample records for purification systems ii

  1. Increase of COP for heat transformer in water purification systems. Part II - Without increasing heat source temperature

    International Nuclear Information System (INIS)

    Romero, R.J.; Siqueiros, J.; Huicochea, A.

    2007-01-01

    The integration of a water purification system allows a heat transformer to increase the actual coefficient of performance, by the reduction of the amount of heat supplied by unit of heat. A new defined COP called COP WP is proposed for the present system, which considers the fraction of heat recycled. Simulation with proven software compares the performance of the modeling of an absorption heat transformer for water purification (AHTWP) operating with water/lithium bromide, as working fluid-absorbent pair. Plots of enthalpy-based coefficients of performance (COP ET ) and water purification coefficient of performance (COP WP ) are shown against absorber temperature for several thermodynamic operating conditions. The results showed that the proposed (AHTWP) system is capable of increasing the original value of COP ET up to 1.6 times its original value by recycling energy from a water purification system. The proposed COP WP allows increments for COP values from any experimental data for water purification or for any other distillation system integrated to a heat transformer, regardless of actual COP A value or working fluid-absorbent pair

  2. Water Purification Systems

    Science.gov (United States)

    1994-01-01

    Clearwater Pool Technologies employs NASA-developed silver/copper ionization to purify turtle and dolphin tanks, cooling towers, spas, water recycling systems, etc. The pool purifier consists of a microcomputer to monitor water conditions, a pair of metallic electrodes, and a rheostat controller. Ions are generated by passing a low voltage current through the electrodes; the silver ions kill the bacteria, and the copper ions kill algae. This technology has found broad application because it offers an alternative to chemical disinfectants. It was originally developed to purify water on Apollo spacecraft. Caribbean Clear has been using NASA's silver ionization technology for water purification for more than a decade. Two new products incorporate advancements of the basic technology. One is the AquaKing, a system designed for areas with no source of acceptable drinking water. Another is the Caribbean Clear Controller, designed for commercial pool and water park applications where sanitizing is combined with feedback control of pH and an oxidizer, chlorine or bromine. The technology was originally developed to purify water on Apollo spacecraft.

  3. Heterologous expression, purification and characterization of human β-1,2-N-acetylglucosaminyltransferase II using a silkworm-based Bombyx mori nucleopolyhedrovirus bacmid expression system.

    Science.gov (United States)

    Miyazaki, Takatsugu; Kato, Tatsuya; Park, Enoch Y

    2018-02-03

    β-1,2-N-Acetylglucosaminyltransferase II (GnTII, EC 2.4.1.143) is a Golgi-localized type II transmembrane enzyme that catalyzes the transfer of N-acetylglucosamine to the 6-arm of the trimanosyl core of N-glycans, an essential step in the conversion of oligomannose-type to complex-type N-glycans. Despite its physiological importance, there have been only a few reports on the heterologous expression and structure-function relationship of this enzyme. Here, we constructed a silkworm-based Bombyx mori nucleopolyhedrovirus bacmid expression system and expressed human GnTII (hGnTII) lacking the N-terminal cytosolic tail and transmembrane region. The recombinant hGnTII was purified from silkworm larval hemolymph in two steps by using tandem affinity purification tags, with a yield of approximately 120 μg from 10 mL hemolymph, and exhibited glycosyltransferase activity and strict substrate specificity. The enzyme was found to be N-glycosylated by the enzymatic cleavage of glycans, while hGnTII expressed in insect cells had not been reported to be glycosylated. Although insects typically produce pauci-mannosidic-type glycans, the structure of N-glycans in the recombinant hGnTII was suggested to be of the complex type, and the removal of the glycans did not affect the enzymatic activity. Copyright © 2018 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

  4. Secondary coolant purification system

    International Nuclear Information System (INIS)

    Stiteler, F.Z.; Donohue, J.P.

    1978-01-01

    The present invention combines the attributes of volatile chemical addition, continuous blowdown, and full flow condensate demineralization. During normal plant operation (defined as no primary to secondary leakage) condensate from the condenser is pumped through a full flow condensate demineralizer system by the condensate pumps. Volatile chemical additions are made. Dissolved and suspended solids are removed in the condensate polishers by ion exchange and/or filtration. At the same time a continuous blowdown of approximately 1 percent of the main steaming rate of the steam generators is maintained. Radiation detectors monitor the secondary coolant. If these monitors indicate no primary to secondary leakage, the blowdown is cooled and returned directly to the condensate pump discharge. If one of the radiation monitors should indicate a primary to secondary leak, when the temperature of the effluent exiting from the blowdown heat exchanger is compatible with the resin specifications of the ion exchangers, the bypass valve causes the blowdown flow to pass through the blowdown ion exchangers

  5. Reverse osmosis water purification system

    Science.gov (United States)

    Ahlstrom, H. G.; Hames, P. S.; Menninger, F. J.

    1986-01-01

    A reverse osmosis water purification system, which uses a programmable controller (PC) as the control system, was designed and built to maintain the cleanliness and level of water for various systems of a 64-m antenna. The installation operates with other equipment of the antenna at the Goldstone Deep Space Communication Complex. The reverse osmosis system was designed to be fully automatic; with the PC, many complex sequential and timed logic networks were easily implemented and are modified. The PC monitors water levels, pressures, flows, control panel requests, and set points on analog meters; with this information various processes are initiated, monitored, modified, halted, or eliminated as required by the equipment being supplied pure water.

  6. Expression and purification of sea raven type II antifreeze protein from Drosophila melanogaster S2 cells.

    Science.gov (United States)

    Scotter, Andrew J; Kuntz, Douglas A; Saul, Michelle; Graham, Laurie A; Davies, Peter L; Rose, David R

    2006-06-01

    We present a system for the expression and purification of recombinant sea raven type II antifreeze protein, a cysteine-rich, C-type lectin-like globular protein that has proved to be a difficult target for recombinant expression and purification. The cDNAs encoding the pro- and mature forms of the sea raven protein were cloned into a modified pMT Drosophila expression vector. These constructs produced N-terminally His(6)-tagged pro- and mature forms of the type II antifreeze protein under the control of a metallothionein promoter when transfected into Drosophila melanogaster S2 cells. Upon induction of stable cell lines the two proteins were expressed at high levels and secreted into the medium. The proteins were then purified from the cell medium in a simple and rapid protocol using immobilized metal affinity chromatography and specific protease cleavage by tobacco etch virus protease. The proteins demonstrated antifreeze activity indistinguishable from that of wild-type sea raven antifreeze protein purified from serum as illustrated by ice affinity purification, ice crystal morphology, and their ability to inhibit ice crystal growth. This expression and purification system gave yields of 95 mg/L of fully active mature sea raven type II AFP and 9.6 mg/L of the proprotein. This surpasses all previous attempts to express this protein in Escherichia coli, baculovirus-infected fall armyworm cells and Pichia pastoris and will provide sufficient protein for structural analysis.

  7. 21 CFR 876.5665 - Water purification system for hemodialysis.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Water purification system for hemodialysis. 876... SERVICES (CONTINUED) MEDICAL DEVICES GASTROENTEROLOGY-UROLOGY DEVICES Therapeutic Devices § 876.5665 Water purification system for hemodialysis. (a) Identification. A water purification system for hemodialysis is a...

  8. Breathing Air Purification for Hyperbaric Purposes, Part II

    Directory of Open Access Journals (Sweden)

    Woźniak Arkadiusz

    2015-03-01

    Full Text Available Determining the efficiency of breathing air purification for hyperbaric purposes with the use of filtration systems is of a crucial importance. However, when the Polish Navy took samples of breathing air from their own filtration plant for quality purposes, these were found to not meet the required standard. The identification of this problem imposed the need to undertake actions aimed at the elimination of the identified disruptions in the process of breathing air production, with the objective of assuring its proper quality. This study presents the results of the initial tests on the air supply sources utilised by the Polish Navy, which were carried out for the purpose of setting a proper direction of future works and implementing corrective measures in order to optimise the breathing air production process. The obtained test results will be used in a subsequent publication devoted to the assessment of the level of efficiency of air purification with the use of a multifaceted approach consisting in the utilisation of various types of air supply sources and different configurations of purification systems.

  9. Recombinant allergen Lol p II: expression, purification and characterization.

    Science.gov (United States)

    Tamborini, E; Brandazza, A; De Lalla, C; Musco, G; Siccardi, A G; Arosio, P; Sidoli, A

    1995-05-01

    Pollen from perennial rye grass (Lolium perenne) is a major cause of type I allergies worldwide. It contains complex mixtures of proteins, among which Lol p II is a major allergen. Previously, we have reported the cloning and sequencing of Lol p II and its expression in fusion with the heavy chain of human ferritin as carrier polypeptide (Sidoli et al., 1993, J. biol. Chem. 268, 21819-21825). Here, we describe the expression, purification and characterization of a recombinant Lol p II overproduced as a non-fusion protein in the periplasm of E. coli. The recombinant allergen was expressed in high yields and was easily purified in milligram amounts. It competed with the natural Lol p II for binding to specific IgE, and it induced allergic responses in skin prick tests, indicating to be immunologically analogous to the natural protein. Biochemical analyses indicate that recombinant Lol p II is a highly stable and soluble monomeric molecule which behaves like a small globular protein.

  10. CAREM-25. Purification and volume control system

    International Nuclear Information System (INIS)

    Acosta, Eduardo; Carlevaris, Rodolfo; Patrignani, Alberto; Chocron, Mauricio; Goya, Hector E.; Ortega, Daniel A.; Ramilo, Lucia B.

    2000-01-01

    The purification and volume control system has the following main functions: water level control inside reactor pressure vessel (RPR) in all the reactor operational modes, pressure control when the reactor operates in solid state, and maintenance of radiological, physical and chemical parameters of primary water. In case of Hot Shutdown operational mode and also after Scram the system is capable of extraction of nuclear decay heat. The design of the system is in accordance with the Requirements of ANSI/ ANS 51.1; 58.11 and 56.2 standards. (author)

  11. Automated Water-Purification System

    Science.gov (United States)

    Ahlstrom, Harlow G.; Hames, Peter S.; Menninger, Fredrick J.

    1988-01-01

    Reverse-osmosis system operates and maintains itself with minimal human attention, using programmable controller. In purifier, membranes surround hollow cores through which clean product water flows out of reverse-osmosis unit. No chemical reactions or phase changes involved. Reject water, in which dissolved solids concentrated, emerges from outer membrane material on same side water entered. Flow controls maintain ratio of 50 percent product water and 50 percent reject water. Membranes expected to last from 3 to 15 years.

  12. A Scintillator Purification System for the Borexino Solar Neutrino Detector

    OpenAIRE

    Benziger, J.; Cadonati, L.; Calaprice, F.; Chen, M.; Corsi, A.; Dalnoki-Veress, F.; Fernholz, R.; Ford, R.; Galbiati, C.; Goretti, A.; Harding, E.; Ianni, Aldo; Ianni, Andrea; Kidner, S.; Leung, M.

    2007-01-01

    Purification of the 278 tons of liquid scintillator and 889 tons of buffer shielding for the Borexino solar neutrino detector was performed with a system that combined distillation, water extraction, gas stripping and filtration. The purification of the scintillator achieved unprecedented low backgrounds for the large scale liquid scintillation detector. This paper describes the principles of operation, design, construction and commissioning of the purification system, and reviews the require...

  13. A scintillator purification system for the Borexino solar neutrino detector

    Science.gov (United States)

    Benziger, J.; Cadonati, L.; Calaprice, F.; Chen, M.; Corsi, A.; Dalnoki-Veress, F.; Fernholz, R.; Ford, R.; Galbiati, C.; Goretti, A.; Harding, E.; Ianni, Aldo; Ianni, Andrea; Kidner, S.; Leung, M.; Loeser, F.; McCarty, K.; McKinsey, D.; Nelson, A.; Pocar, A.; Salvo, C.; Schimizzi, D.; Shutt, T.; Sonnenschein, A.

    2008-03-01

    Purification of the 278 tons of liquid scintillator and 889 tons of buffer shielding for the Borexino solar neutrino detector is performed with a system that combines distillation, water extraction, gas stripping, and filtration. This paper describes the principles of operation, design, and construction of that purification system, and reviews the requirements and methods to achieve system cleanliness and leak-tightness.

  14. The Protein Maker: an automated system for high-throughput parallel purification

    International Nuclear Information System (INIS)

    Smith, Eric R.; Begley, Darren W.; Anderson, Vanessa; Raymond, Amy C.; Haffner, Taryn E.; Robinson, John I.; Edwards, Thomas E.; Duncan, Natalie; Gerdts, Cory J.; Mixon, Mark B.; Nollert, Peter; Staker, Bart L.; Stewart, Lance J.

    2011-01-01

    The Protein Maker instrument addresses a critical bottleneck in structural genomics by allowing automated purification and buffer testing of multiple protein targets in parallel with a single instrument. Here, the use of this instrument to (i) purify multiple influenza-virus proteins in parallel for crystallization trials and (ii) identify optimal lysis-buffer conditions prior to large-scale protein purification is described. The Protein Maker is an automated purification system developed by Emerald BioSystems for high-throughput parallel purification of proteins and antibodies. This instrument allows multiple load, wash and elution buffers to be used in parallel along independent lines for up to 24 individual samples. To demonstrate its utility, its use in the purification of five recombinant PB2 C-terminal domains from various subtypes of the influenza A virus is described. Three of these constructs crystallized and one diffracted X-rays to sufficient resolution for structure determination and deposition in the Protein Data Bank. Methods for screening lysis buffers for a cytochrome P450 from a pathogenic fungus prior to upscaling expression and purification are also described. The Protein Maker has become a valuable asset within the Seattle Structural Genomics Center for Infectious Disease (SSGCID) and hence is a potentially valuable tool for a variety of high-throughput protein-purification applications

  15. Submersible purification system for radioactive water

    Science.gov (United States)

    Abbott, Michael L.; Lewis, Donald R.

    1989-01-01

    A portable, submersible water purification system for use in a pool of water containing radioactive contamination includes a prefilter for filtering particulates from the water. A resin bed is then provided for removal of remaining dissolved, particulate, organic, and colloidal impurities from the prefiltered water. A sterilizer then sterilizes the water. The prefilter and resin bed are suitably contained and are submerged in the pool. The sterilizer is water tight and located at the surface of the pool. The water is circulated from the pool through the prefilter, resin bed, and sterilizer by suitable pump or the like. In the preferred embodiment, the resin bed is contained within a tank which stands on the bottom of the pool and to which a base mounting the prefilter and pump is attached. An inlet for the pump is provided adjacent the bottom of the pool, while the sterilizer and outlet for the system is located adjacent the top of the pool.

  16. A new helium gas recovery and purification system

    International Nuclear Information System (INIS)

    Yamamotot, T.; Suzuki, H.; Ishii, J.; Hamana, I.; Hayashi, S.; Mizutani, S.; Sanjo, S.

    1974-01-01

    A helium gas recovery and purification system, based on the principle of gas permeation through a membrane, is described. The system can be used for the purification of helium gas containing air as a contaminant. The apparatus, operating at ambient temperature does not need constant attention, the recovery ratio of helium gas is satisfactory and running costs are low. Gases other than helium can be processed with the apparatus. (U.K.)

  17. Purification

    DEFF Research Database (Denmark)

    Andersen, Astrid Oberborbeck

    2017-01-01

    In Arequipa, Peru’s second largest city, engineers work hard to control water flows and provide different sectors with clean and sufficient water. In 2011, only 10 percent of the totality of water used daily by Arequipa’s then close to 1 million people—in households, tourism, industry, and mining......—was treated before it was returned to the river where it continues its flow downstream towards cultivated fields and, finally, into the Pacific Ocean. It takes specialized knowledge and manifold technologies to manage water and sustain life in Arequipa, and engineers are central actors for making water flow...... of categories can be understood as practices of purification. However, a purely technical grip on water is never possible. Unruly elements, like weather, contamination, urban dwellers, and competing interests, interfere and make processes of intervention unstable. Water is never completely cleaned, and, equally...

  18. Fission-product transfer in the TMI-2 purification system

    International Nuclear Information System (INIS)

    Cox, T.E.

    1982-01-01

    The makeup purification system at TMI-2 operated during the course of the accident, processing water from the reactor coolant system cold leg at an average flow rate not exceeding 4.4 x 10 - 3 m 3 /s. The system operated through most of 28 March 1979, finally being shutdown when the system filters or demineralizers, or both, plugged and overpressured. The system was restored to service on 29 March 1979 at a flow rate of about 1.6 x 10 - 3 m 3 /s. Subsequent radiation readings of the system filters and demineralizer cubicles revealed that these components contained appreciable levels of radionuclides. One project being implemented within the Radiation and Environment Program of the Technical Integration Office is to analyze the demineralizer resins and filters, as they are removed from the makeup purification system. The object is to determine the quantity and composition of the material retained by the resins and filters

  19. 21 CFR 884.6170 - Assisted reproduction water and water purification systems.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Assisted reproduction water and water purification... Devices § 884.6170 Assisted reproduction water and water purification systems. (a) Identification. Assisted reproduction water purification systems are devices specifically intended to generate high quality...

  20. First Study of Helium Gas Purification System as Primary Coolant of Co-Generation Reactor

    International Nuclear Information System (INIS)

    Piping Supriatna

    2009-01-01

    The technological progress of NPP Generation-I on 1950’s, Generation-II, Generation-III recently on going, and Generation-IV which will be implemented on next year 2025, concept of nuclear power technology implementation not only for generate electrical energy, but also for other application which called cogeneration reactor. Commonly the type of this reactor is High Temperature Reactor (HTR), which have other capabilities like Hydrogen production, desalination, Enhanced Oil Recovery (EOR), etc. The cogeneration reactor (HTR) produce thermal output higher than commonly Nuclear Power Plant, and need special Heat Exchanger with helium gas as coolant. In order to preserve heat transfer with high efficiency, constant purity of the gas must be maintained as well as possible, especially contamination from its impurities. In this report has been done study for design concept of HTR primary coolant gas purification system, including methodology by sampling He gas from Primary Coolant and purification by using Physical Helium Splitting Membrane. The examination has been designed in physical simulator by using heater as reactor core. The result of study show that the of Primary Coolant Gas Purification System is enable to be implemented on cogeneration reactor. (author)

  1. Replacement of fine particle purification filter of the PHT purification system - 15083

    International Nuclear Information System (INIS)

    Lee, D.S.

    2015-01-01

    The increase of chalk river unidentified deposit (CRUD), a particulate corrosion product in PHT (primary heat transport) system with increased operating years of a nuclear power plant causes: -) the problems of increased heavy water decomposition and deuterium formation reaction due to catalytic reaction with CRUD, -) damage to PHT pump seal due to a corrosion product, -) damage to fuel channel closure seal, and increased radiation exposure of workers due to elevated dose rate in steam generator water chamber. Wolsung unit 3 and 4 have replaced fine filter media in PHT purification system in phases reducing the pore size of the filter media (5 μm → 2 μm → 1 μm → 0.45 μm) to solve this problem. The phased replacement of fine filter media by the one with a smaller pore size reduced CRUD in PHT system significantly and also radiation dose rate in steam generator water chamber. Accordingly, many problems related to the aging of a plant (including increased radiation exposure of workers during outage, damage to mechanical seal of PHT pump) have been solved. (author)

  2. Operating experiences of gas purification system of Heavy Water Plant Talcher (Paper No. 1.11)

    International Nuclear Information System (INIS)

    Bhattacharya, R.; Mohanty, P.R.; Pandey, B.L.

    1992-01-01

    The operating experiences with the purification system installed at Heavy Water Plant, Talcher for purification of feed synthesis gas from fertilizer plant is described. The purification system has performed satisfactorily even with levels of impurities as much as 15 to 20 ppm of oxygen and carbon monoxide. The system could not however be tested at designed gas throughput and on a sustained basis. However, increase in gas throughput upto the design value is not expected to pose any problem on the performance of the purification system. (author). 5 figs

  3. Systems and methods for separation and purification of products

    Energy Technology Data Exchange (ETDEWEB)

    Weiss, Michael Joseph; Gilliam, Ryan J.; Self, Kyle; Mariansky, Gal; Leclerc, Margarete K.; Shipchandler, Riyaz Mohammed; Nagar, Jacob

    2017-11-28

    There are provided methods and systems for an electrochemical cell including an anode and a cathode where the anode is contacted with a metal ion that converts the metal ion from a lower oxidation state to a higher oxidation state. The metal ion in the higher oxidation state is reacted with an unsaturated hydrocarbon and/or a saturated hydrocarbon to form products. Separation and/or purification of the products as well as of the metal ions in the lower oxidation state and the higher oxidation state, is provided herein.

  4. New research on bioregenerative air/water purification systems

    Science.gov (United States)

    Johnson, Anne H.; Ellender, R. D.; Watkins, Paul J.

    1991-01-01

    For the past several years, air and water purification systems have been developed and used. This technology is based on the combined activities of plants and microorganisms as they function in a natural environment. More recently, researchers have begun to address the problems associated with indoor air pollution. Various common houseplants are currently being evaluated for their abilities to reduce concentrations of volatile organic compounds (VOCS) such as formaldehyde and benzene. With development of the Space Exploration Initiative, missions will increase in duration, and problems with resupply necessitates implementation of regenerative technology. Aspects of bioregenerative technology have been included in a habitat known as the BioHome. The ultimate goal is to use this technology in conjunction with physicochemical systems for air and water purification within closed systems. This study continued the risk assessment of bioregenerative technology with emphasis on biological hazards. In an effort to evaluate the risk for human infection, analyses were directed at enumeration of fecal streptococci and enteric viruses with the BioHome waste water treatment system.

  5. Air Purification in Closed Environments: An Overview of Spacecraft Systems

    Science.gov (United States)

    Perry, Jay L.; LeVan, Douglas; Crumbley, Robert (Technical Monitor)

    2002-01-01

    The primary goal for a collective protection system and a spacecraft environmental control and life support system (ECLSS) are strikingly similar. Essentially both function to provide the occupants of a building or vehicle with a safe, habitable environment. The collective protection system shields military and civilian personnel from short-term exposure to external threats presented by toxic agents and industrial chemicals while an ECLSS sustains astronauts for extended periods within the hostile environment of space. Both have air quality control similarities with various aircraft and 'tight' buildings. This paper reviews basic similarities between air purification system requirements for collective protection and an ECLSS that define surprisingly common technological challenges and solutions. Systems developed for air revitalization on board spacecraft are discussed along with some history on their early development as well as a view of future needs. Emphasis is placed upon two systems implemented by the National Aeronautics and Space Administration (NASA) onboard the International Space Station (ISS): the trace contaminant control system (TCCS) and the molecular sieve-based carbon dioxide removal assembly (CDRA). Over its history, the NASA has developed and implemented many life support systems for astronauts. As the duration, complexity, and crew size of manned missions increased from minutes or hours for a single astronaut during Project Mercury to days and ultimately months for crews of 3 or more during the Apollo, Skylab, Shuttle, and ISS programs, these systems have become more sophisticated. Systems aboard spacecraft such as the ISS have been designed to provide long-term environmental control and life support. Challenges facing the NASA's efforts include minimizing mass, volume, and power for such systems, while maximizing their safety, reliability, and performance. This paper will highlight similarities and differences among air purification systems

  6. Modeling Xenon Purification Systems in a Laser Inertial Fusion Engine

    Science.gov (United States)

    Hopkins, Ann; Gentile, Charles

    2011-10-01

    A Laser Inertial Fusion Engine (LIFE) is a proposed method to employ fusion energy to produce electricity for consumers. However, before it can be built and used as such, each aspect of a LIFE power plant must first be meticulously planned. We are in the process of developing and perfecting models for an exhaust processing and fuel recovery system. Such a system is especially essential because it must be able to recapture and purify expensive materials involved in the reaction so they may be reused. One such material is xenon, which is to be used as an intervention gas in the target chamber. Using Aspen HYSYS, we have modeled several subsystems for exhaust processing, including a subsystem for xenon recovery and purification. After removing hydrogen isotopes using lithium bubblers, we propose to use cryogenic distillation to purify the xenon from remaining contaminants. Aspen HYSYS allows us to analyze predicted flow rates, temperatures, pressures, and compositions within almost all areas of the xenon purification system. Through use of Aspen models, we hope to establish that we can use xenon in LIFE efficiently and in a practical manner.

  7. CAREM 25: Suppression pool cooling and purification system

    International Nuclear Information System (INIS)

    Carlevaris, Rodolfo; Patrignani, Alberto; Vindrola, Carlos; Palmerio, Hector D.; Quiroz, Horacio; Ramilo, Lucia B.

    2000-01-01

    The suppression pool cooling and purification system has the following main functions: purify and cool water from the suppression pool, cool and send water to the residual heat extraction system, and transfer water to the fuel element transference channel. In case of Loss of Coolant Accident (LOCA), the system sends water from the suppression pool to the spray network, thus cooling and reducing pressure in the primary containment. The system has been designed in accordance with the requirements of the following standards: ANSI/ANS 52.1; ANSI/ANS 57.2; ANSI/ANS 56.2; ANSI/ANS 59.1; ANSI/ANS 58.3; ANSI/ANS 58.9; and ANSI/ANS 56.5. The design of the system fulfils all the assigned functions. (author)

  8. CAREM-25. Suppression Pool Cooling and Purification System

    International Nuclear Information System (INIS)

    Carlevaris, Rodolfo; Palmerio, D.; Patrignani, A.; Quiroz, H.; Ramilo, L.; Vindrola, C.

    2000-01-01

    The Suppression Pool Cooling and Purification System has the following main functions: purify and cool water from the Suppression Pool, cool and send water to the Residual Heat Extraction System, and transfer water to the Fuel Element Transference Channel. In case of Loss of Coolant Accident (LOCA), the system sends water from the Suppression Pool to the spray network, thus cooling and reducing pressure in the primary containment.The system has been designed in accordance with the requirements of the following standards ANSI/ANS 52.1 [1], ANSI/ANS 57.2 [2], ANSI/ANS 56.2 [3], ANSI/ANS 59.1 [4] ANSI/ANS 58.3 [5], ANSI/ANS 58.9 [6], and ANSI/ANS 56.5 [7]. The design of the system fulfils all the assigned functions

  9. Two-phase aqueous micellar systems: an alternative method for protein purification

    Directory of Open Access Journals (Sweden)

    Rangel-Yagui C. O.

    2004-01-01

    Full Text Available Two-phase aqueous micellar systems can be exploited in separation science for the extraction/purification of desired biomolecules. This article reviews recent experimental and theoretical work by Blankschtein and co-workers on the use of two-phase aqueous micellar systems for the separation of hydrophilic proteins. The experimental partitioning behavior of the enzyme glucose-6-phosphate dehydrogenase (G6PD in two-phase aqueous micellar systems is also reviewed and new results are presented. Specifically, we discuss very recent work on the purification of G6PD using: i a two-phase aqueous micellar system composed of the nonionic surfactant n-decyl tetra(ethylene oxide (C10E4, and (ii a two-phase aqueous mixed micellar system composed of C10E4 and the cationic surfactant decyltrimethylammonium bromide (C10TAB. Our results indicate that the two-phase aqueous mixed (C10E4/C10TAB micellar system can improve significantly the partitioning behavior of G6PD relative to that observed in the two-phase aqueous C10E4 micellar system.

  10. Reactor coolant purification system circulation pumps (CUW pumps)

    International Nuclear Information System (INIS)

    Tsutsui, Toshiaki

    1979-01-01

    Coolant purification equipments for BWRs have been improved, and the high pressure purifying system has become the main type. The quantity of purifying treatment also changed to 2% of the flow rate of reactor feed water. As for the circulation pumps, canned motor pumps are adopted recently, and the improvements of reliability and safety are attempted. The impurities carried in by reactor feed water and the corrosion products generated in reactors and auxiliary equipments are activated by neutron irradiation or affect heat transfer adversely, adhering to fuel claddings are core structures. Therefore, a part of reactor coolant is led to the purification equipments, and returned to reactors after the impurities are eliminated perfectly. At the time of starting and stopping reactors, excess reactor water and the contaminated water from reactors are transferred to main condenser hot wells or waste treatment systems. Thus the prescribed water quality is maintained. The operational modes of and the requirements for the CUW pumps, the construction and the features of the canned motor type CUW pumps are explained. Recently, a pump operated for 11 months without any maintenance has been disassembled and inspected, but the wear of bearings has not been observed, and the high reliability of the pump has been proved. (Kako, I.)

  11. CO 2-scrubbing and methanation as purification system for PEFC

    Science.gov (United States)

    Ledjeff-Hey, K.; Roes, J.; Wolters, R.

    Hydrogen is usually produced by steam reforming of natural gas in large-scale processes. The reformate consists of hydrogen, carbon dioxide, carbon monoxide, and residues of hydrocarbons. Since the anode catalyst of a polymer electrolyte membrane fuel cell (PEFC) is usually based on platinum, which is easily poisoned by carbon monoxide, the conditioned feed gas should contain less than 100 ppmv CO, and preferably, less than 10 ppmv. Depending on the design and operating conditions of the hydrogen production process, the CO content of a typical reformate gas, even after the CO shift reactor may be in the range of 0.2-1.0 vol.%; this is far higher than a PEFC can tolerate. A CO management system is required to lower the CO concentration to acceptable levels. In many cases, the CO purification system consists of a combination of physical or chemical processes to achieve the necessary reduction in CO content. A promising alternative for hydrogen purification is a combined process consisting of a carbon dioxide scrubber with subsequent methanation to reduce the carbon monoxide content to an acceptable level of less than 10 ppmv.

  12. The assessment of helium purification system of small power HTGR

    International Nuclear Information System (INIS)

    Siti Alimah; Sriyono

    2016-01-01

    The helium purification system (HPS) is one of safety system of High Temperature Gas-cooled Reactor. HPS removes impurities in the primary coolant, so that the impact on structure, system and component (SSC) is minimized. The two impurity types are particulates (carbon dust, fission products (Kr, Xe, Cs etc.) and the gases (O_2, N_2, H_2O, CH_4, CO, CO_2 and H_2). Every reactor has a different impurity limit during normal operation, depends on the reactor power, energy conversion system and fuel type. This paper discusses the HPS on HTR-10, HTTR and Indonesian RDE conceptual design. The purpose of this assessment is to determine the optimum HPS design as a role model for Indonesian RDE. The utilized methodology is a literature study based on the operating experiences of both HTR-10 and HTTR as well as the evaluation of RDE conceptual design. This study focuses on the impurities limit during normal operation, the main components of HPS, mass flow-rate and regeneration process. The main component that used in HPS for HTR-10, HTTR and RDE are similar i.e. filter, CuO column, water cooler, molecular sieve bed and cryogenic activated carbon bed. Refer to the HTR-10 and HTTR operational experiences, both of those reactors have a purification systems that capable to maintain the helium purity, even though the impurities limit are different. The HPS of HTTR Japan has a stricter impurities limit that N_2, CH_4, and O_2 should not be contained at all during normal operation and the pre-charcoal trap is used to adsorb the fine dust below 0.1 micron. Both of these parameters can be adopted to the RDE's HPS design to minimize the effect of impurities to SSC. (author)

  13. A purification process for an inert gas system

    International Nuclear Information System (INIS)

    Raj, S.S.; Samanta, S.K.; Jain, N.G.; Deshingkar, D.S.; Ramaswamy, M.

    1984-01-01

    Special inert atmosphere is desired inside hot cells used for handling radioactive materials. In this report, details of experiments conducted to generate data required for the design of a system for maintaining very low levels of organic and acid vapours, oxygen and moisture in a nitrogen gas inert atmosphere, are described. Several grades of activated charcoals impregnated with 1% KOH were studied for the adsorption of acidic and organic vapours. A Pd/Al 2 O 3 catalyst was developed to remove oxygen with greater than 90% efficiency. For the removal of moisture, a regenerable molecular sieve 4A dual-bed was provided. Based on the performance data thus generated, an integrated purification system for nitrogen gas is proposed. (author)

  14. Expression, purification and characterization of enoyl-ACP reductase II, FabK, from Porphyromonas gingivalis

    Energy Technology Data Exchange (ETDEWEB)

    Hevener, Kirk E.; Mehboob, Shahila; Boci, Teuta; Truong, Kent; Santarsiero, Bernard D.; Johnson, Michael E. (UIC)

    2012-10-25

    The rapid rise in bacterial drug resistance coupled with the low number of novel antimicrobial compounds in the discovery pipeline has led to a critical situation requiring the expedient discovery and characterization of new antimicrobial drug targets. Enzymes in the bacterial fatty acid synthesis pathway, FAS-II, are distinct from their mammalian counterparts, FAS-I, in terms of both structure and mechanism. As such, they represent attractive targets for the design of novel antimicrobial compounds. Enoyl-acyl carrier protein reductase II, FabK, is a key, rate-limiting enzyme in the FAS-II pathway for several bacterial pathogens. The organism, Porphyromonas gingivalis, is a causative agent of chronic periodontitis that affects up to 25% of the US population and incurs a high national burden in terms of cost of treatment. P. gingivalis expresses FabK as the sole enoyl reductase enzyme in its FAS-II cycle, which makes this a particularly appealing target with potential for selective antimicrobial therapy. Herein we report the molecular cloning, expression, purification and characterization of the FabK enzyme from P. gingivalis, only the second organism from which this enzyme has been isolated. Characterization studies have shown that the enzyme is a flavoprotein, the reaction dependent upon FMN and NADPH and proceeding via a Ping-Pong Bi-Bi mechanism to reduce the enoyl substrate. A sensitive assay measuring the fluorescence decrease of NADPH as it is converted to NADP{sup +} during the reaction has been optimized for high-throughput screening. Finally, protein crystallization conditions have been identified which led to protein crystals that diffract x-rays to high resolution.

  15. Staphylococcal phosphoenolpyruvate-dependent phosphotransferase system: purification and characterization of the mannitol-specific enzyme III/sup mtl/ of Staphylococcus aureus and Staphylococcus carnosus and homology with the enzyme II/sup mtl/ of Escherichia coli

    International Nuclear Information System (INIS)

    Reiche, B.; Frank, R.; Deutscher, J.; Meyer, N.; Hengstenberg, W.

    1988-01-01

    Enzyme III/sup mtl/ is part of the mannitol phosphotransferase system of Staphylococcus aureus and Staphylococcus carnosus and is phosphorylated by phosphoenolpyruvate in a reaction sequence requiring enzyme I (phosphoenolpyruvate-protein phosphotransferase) and the histidine-containing protein HPr. In this paper, the authors report the isolation of III/sup mtl/ from both S. aureus and S. carnosus and the characterization of the active center. After phosphorylation of III/sup mtl/ with [ 32 P]PEP, enzyme I, and HPr, the phosphorylated protein was cleaved with endoproteinase GLu(C). The amino acid sequence of the S. aureus peptide carrying the phosphoryl group was found to be Gln-Val-Val-Ser-Thr-Phe-Met-Gly-Asn-Gly-Leu-Ala-Ile-Pro-His-Gly-Thr-Asp-Asp. The corresponding peptide from S. carnosus shows an equal sequence except that the first residue is Ala instead of Gln. These peptides both contain a single histidyl residue which they assume to carry the phosphoryl group. All proteins of the PTS so far investigated indeed carry the phosphoryl group attached to a histidyl residue. According to sodium dodecyl sulfate gels, the molecular weight of the III/sup mtl/ proteins was found to be 15,000. They have also determined the N-terminal sequence of both proteins. Comparison of the III/sup mtl/ peptide sequences and the C-terminal part of the enzyme II/sup mtl/ of Escherichia coli reveals considerable sequence homology, which supports the suggestion that II/sup mtl/ of E. coli is a fusion protein of a soluble III protein with a membrane-bound enzyme II

  16. Cloning, overexpression, purification and preliminary crystallographic studies of a mitochondrial type II peroxiredoxin from Pisum sativum

    International Nuclear Information System (INIS)

    Barranco-Medina, Sergio; López-Jaramillo, Francisco Javier; Bernier-Villamor, Laura; Sevilla, Francisca; Lázaro, Juan-José

    2006-01-01

    The isolation, purification, crystallization and molecular-replacement solution of mitochondrial type II peroxiredoxin from P. sativum is reported. A cDNA encoding an open reading frame of 199 amino acids corresponding to a type II peroxiredoxin from Pisum sativum with its transit peptide was isolated by RT-PCR. The 171-amino-acid mature protein (estimated molecular weight 18.6 kDa) was cloned into the pET3d vector and overexpressed in Escherichia coli. The recombinant protein was purified and crystallized by the hanging-drop vapour-diffusion technique. A full data set (98.2% completeness) was collected using a rotating-anode generator to a resolution of 2.8 Å from a single crystal flash-cooled at 100 K. X-ray data revealed that the protein crystallizes in space group P1, with unit-cell parameters a = 61.88, b = 66.40, c = 77.23 Å, α = 102.90, β = 104.40, γ = 99.07°, and molecular replacement using a theoretical model predicted from the primary structure as a search model confirmed the presence of six molecules in the unit cell as expected from the Matthews coefficient. Refinement of the structure is in progress

  17. Cloning, overexpression, purification and preliminary crystallographic studies of a mitochondrial type II peroxiredoxin from Pisum sativum

    Energy Technology Data Exchange (ETDEWEB)

    Barranco-Medina, Sergio [Departamento de Bioquímica, Biología Molecular y Celular de Plantas, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, E-18008 Granada (Spain); López-Jaramillo, Francisco Javier, E-mail: fjljara@ugr.es [Instituto de Biotecnología, Campus Fuentenueva, Universidad de Granada, E-18071 Granada (Spain); Bernier-Villamor, Laura [Departamento de Bioquímica, Biología Molecular y Celular de Plantas, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, E-18008 Granada (Spain); Sevilla, Francisca [Departamento de Biología del Estrés y Patología Vegetal, Centro de Edafología y Biología Aplicada del Segura, Consejo Superior de Investigaciones Científicas, E-30080 Murcia (Spain); Lázaro, Juan-José [Departamento de Bioquímica, Biología Molecular y Celular de Plantas, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, E-18008 Granada (Spain)

    2006-07-01

    The isolation, purification, crystallization and molecular-replacement solution of mitochondrial type II peroxiredoxin from P. sativum is reported. A cDNA encoding an open reading frame of 199 amino acids corresponding to a type II peroxiredoxin from Pisum sativum with its transit peptide was isolated by RT-PCR. The 171-amino-acid mature protein (estimated molecular weight 18.6 kDa) was cloned into the pET3d vector and overexpressed in Escherichia coli. The recombinant protein was purified and crystallized by the hanging-drop vapour-diffusion technique. A full data set (98.2% completeness) was collected using a rotating-anode generator to a resolution of 2.8 Å from a single crystal flash-cooled at 100 K. X-ray data revealed that the protein crystallizes in space group P1, with unit-cell parameters a = 61.88, b = 66.40, c = 77.23 Å, α = 102.90, β = 104.40, γ = 99.07°, and molecular replacement using a theoretical model predicted from the primary structure as a search model confirmed the presence of six molecules in the unit cell as expected from the Matthews coefficient. Refinement of the structure is in progress.

  18. Regenerable Air Purification System for Gas-Phase Contaminant Control

    Science.gov (United States)

    Constantinescu, Ileana C.; Finn, John E.; LeVan, M. Douglas; Lung, Bernadette (Technical Monitor)

    2000-01-01

    Tests of a pre-prototype regenerable air purification system (RAPS) that uses water vapor to displace adsorbed contaminants from an adsorbent column have been performed at NASA Ames Research Center. A unit based on this design can be used for removing trace gas-phase contaminants from spacecraft cabin air or from polluted process streams including incinerator exhaust. During the normal operation mode, contaminants are removed from the air on the column. Regeneration of the column is performed on-line. During regeneration, contaminants are displaced and destroyed inside the closed oxidation loop. In this presentation we discuss initial experimental results for the performance of RAPS in the removal and treatment of several important spacecraft contaminant species from air.

  19. Mechanical damage and corrosion in the primary system purification cooler

    International Nuclear Information System (INIS)

    Sainz, R.A.; Fiorini, R.H.; Semino, C.J.

    1987-01-01

    Through the routine measurements of tritium activity and isotopic content in the exchanger's cooling water, a loss of heavy water was detected. During the decommissioning programmed for October 1986, the equipment was dismantled and the tubes losses were identified through helium fugue tests; subsequently, a 100% inspection of the tubes by atmospheric currents were performed, verifying reductions exceeding 50% of the original width in 70 tubes of the first section at the top plate level. These indications were verified through the study of the two extracted tubes, one of them observing a passing failure where marks appeared at all levels of the support and corrosion plates through splits at the top plates level. The corrosion causes were due to the low cooling flow which results from the primary system's reduction regarding the purification flow design, thus permitting the deposits accumulation. (Author)

  20. A completely passive continuous flow solar water purification system

    Energy Technology Data Exchange (ETDEWEB)

    Duff, William S.; Hodgson, David A. [Dept. of Mechanical Enginnering, Colorado State Univ., Fort Collins, CO (United States)

    2008-07-01

    Water-borne pathogens in developing countries cause several billion cases of disease and up to 10 million deaths each year, at least half of which are children. Solar water pasteurization is a potentially cost-effective, robust and reliable solution to these problems. A completely passively controlled solar water pasteurization system with a total collector area of 0.45 m{sup 2} has been constructed. The system most recently tested produced 337 litres per m{sup 2} of collector area of treated water on a sunny day. We developed our completely passive density-driven solar water pasteurization system over a five year span so that it now achieves reliable control for all possible variations in solar conditions. We have also substantially increased its daily pure water production efficiency over the same period. We will discuss the performance of our water purification system and provide an analyses that demonstrates that the system insures safe purified water production at all times. (orig.)

  1. The effect of water purification systems on fluoride content of drinking water

    Directory of Open Access Journals (Sweden)

    Prabhakar A

    2008-03-01

    Full Text Available Objective: The purpose of the present study was to determine the effect of different water purification systems on the fluoride content of drinking water and to compare the efficacy of these water purification systems in reducing the fluoride content. Materials and Methods: Five different water purification systems were tested in this study. They were reverse osmosis, distillation, activated carbon, Reviva ® , and candle filter. The water samples in the study were of two types, viz, borewell water and tap water, these being commonly used by the people of Davangere City, Karnataka. The samples were collected before and after purification, and fluoride analysis was done using fluoride ion-specific electrode. Results: The results showed that the systems based on reverse osmosis, viz, reverse osmosis system and Reviva ® showed maximum reduction in fluoride levels, the former proving to be more effective than the latter; followed by distillation and the activated carbon system, with the least reduction being brought about by candle filter. The amount of fluoride removed by the purification system varied between the system and from one source of water to the other. Interpretation and Conclusion: Considering the beneficial effects of fluoride on caries prevention; when drinking water is subjected to water purification systems that reduce fluoride significantly below the optimal level, fluoride supplementation may be necessary. The efficacy of systems based on reverse osmosis in reducing the fluoride content of water indicates their potential for use as defluoridation devices.

  2. Health physics system scheme for the uranium purification plant

    International Nuclear Information System (INIS)

    Meyer, M.; Oliveira, E.C.; Sordi, G.A.A.; Abrao, A.

    1976-01-01

    After describing the two uranium purification processes used in the Chemical Engineerring Division of the Instituto de Energia Atomica, it is examined the possible hazards and methods to control or eliminate them. Since these purification process present several stages, in each one of them it is evaluated the hazards and tried to give adequate solutions to protect both, personnel and installations, from the potential radiation hazards

  3. Undulative induction electron accelerator for the waste and natural water purification systems

    CERN Document Server

    Kulish, Victor V; Gubanov, I V

    2001-01-01

    The project analysis of Undulative Induction Accelerator (EH - accelerator) for the waste and natural water purification systems is accomplished. It is shown that the use of the four-channel design of induction block and the standard set of auxiliary equipment (developed earlier for the Linear Induction Accelerators - LINACs) allow to construct commercially promising purification systems. A quality analysis of the accelerator is done and the optimal parameters are chosen taking into account the specific sphere of its usage.

  4. Aerospace Systems Monitor, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — Proposal Title: Aerospace Systems Monitor PHASE 1 Technical Abstract: This Phase II STTR project will continue development and commercialization of the Aerospace...

  5. Fouling issues within CANDU fuel bay purification systems

    International Nuclear Information System (INIS)

    Angell, P.; Murray, J.

    2003-01-01

    Similar to all nuclear power stations, CANDU stations store their spent fuel in large open fuel bays to allow the fuel bundles to cool down. Over the years there have been a number of minor issues relating to the operation of the fuel bays, and the annual operational and maintenance costs to the stations are in the order of $1-2M. Ontario Power Generation (OPG) stations have been treating the fuel bays of their CANDU stations with hydrogen peroxide. While this treatment has been effective for the control of microbial corrosion and algal problems, an ongoing problem with the rapid plugging of the purification system filters and the presence of a thin translucent film on the surface of the water has been reported. Analysis suggested that the translucent film was of biological origin and initially it was suspected as being responsible for the plugging of the filters. Samples of the bay water, translucent film and filters were collected for microbial analysis. From all samples a number of bacterial species was isolated, all of which had the enzyme catalase that is responsible for the microbial breakdown of hydrogen peroxide, and many of the isolates were found to be capable of producing extracellular polymeric substance (slime). Observation of the filter revealed that no slime layer was present on the outer surface of the filter. This suggested that the plugging was not the result of removal of slime from the bay. Rather it suggested that the plugging was the result of microbial colonization and growth within the matrix of the filter. The large surface area of the filter matrix provides ideal conditions for biofilm growth and the flow of water provides a ready supply of nutrients. Based on a careful review of the chemistry specifications and requirements for the fuel bay water it was suggested that the filters, as currently used, were unnecessary and could be either removed or valved out, thereby eliminating the high costs associated with their operation and

  6. Design of a nitrogen purification system with cryogenic method for neutrino detection.

    Science.gov (United States)

    Peng, Zhang; Zhongjun, Hu; Bingming, Wang; Qing, Li

    2018-04-04

    In order to detect the neutrino with liquid scintillation detector, high-purity nitrogen is essential for gas stripping in this detector. Therefore, it is necessary to design a purification system for the detector to purify nitrogen. Using the method of low temperature adsorption for the purification system, the key designs including the flow path, the adsorber and the selection of activated carbon, are introduced in this study. In these designs, the selection of activated carbon is the most important because the adsorption characteristic of the carbon is related to the performance of the purification system. The method of grand canonical ensemble Monte Carlo is adopted to simulate the adsorption of radon by the activated carbon with its slit pore model. Using this method, the working temperature and the key characteristic of the activated carbon are obtained. Copyright © 2018 Elsevier Ltd. All rights reserved.

  7. Quantitative Analysis of Radionuclide for the Used Resin of the Primary Purification System in HANARO

    International Nuclear Information System (INIS)

    Lee, Mun; Kim, Myong Seop; Park, Se Il; Kim, Tae Whan; Kim, Dong Hun; Kim, Young Chil

    2005-01-01

    In HANARO, a 30 MW research reactor, the ion exchange resin has been used for the purification of the primary coolant system. The resin used in the primary coolant purification system is replaced with new one once every 3 months during 30 MW reactor operation. The extracted resin from the primary coolant purification system is temporarily stored in a shielding treatment of the reactor hall for radiation cooling. After the radiation level of resin decreases enough to be handled for the waste disposal, it is put into the waste drum, and delivered to the waste facility in KAERI. Recently, in this procedure, the quantitative analysis of radionuclide which is contained in resin is required to have more quantitative data for the disposal. Therefore, in this work, a preliminary study was performed to find a sampling method for the representation of the characteristics of radionuclide in the spent resin

  8. The importance of the ammonia purification process in ammonia-water absorption systems

    International Nuclear Information System (INIS)

    Fernandez-Seara, Jose; Sieres, Jaime

    2006-01-01

    Practical experience in working with ammonia-water absorption systems shows that the ammonia purification process is a crucial issue in order to obtain an efficient and reliable system. In this paper, the detrimental effects of the residual water content in the vapour refrigerant are described and quantified based on the system design variables that determine the effectiveness of the purification process. The study has been performed considering a single stage system with a distillation column with complete condensation. The ammonia purification effectiveness of the column is analysed in terms of the efficiencies in the stripping and rectifying sections and the reflux ratio. By varying the efficiencies from 0 to 1, systems with neither the rectifying nor stripping section, with either the rectifying or stripping section, or with both sections can be considered. The impact of the ammonia purification process on the absorption system performance is studied based on the column efficiencies and reflux ratio; and its effects on refrigerant concentration, system COP, system pressures and main system mass flow rates and concentrations are analysed. When the highest efficiency rectifying sections are used a combination of generation temperature and reflux ratio which leads to optimum COP values is found. The analysis covers different operating conditions with air and water cooled systems from refrigeration to air conditioning applications by changing the evaporation temperature. The importance of rectification in each kind of application is evaluated

  9. Chemical resistance of the gram-negative bacteria to different sanitizers in a water purification system

    Directory of Open Access Journals (Sweden)

    Penna Thereza CV

    2006-08-01

    Full Text Available Abstract Background Purified water for pharmaceutical purposes must be free of microbial contamination and pyrogens. Even with the additional sanitary and disinfecting treatments applied to the system (sequential operational stages, Pseudomonas aeruginosa, Pseudomonas fluorescens, Pseudomonas alcaligenes, Pseudomonas picketti, Flavobacterium aureum, Acinetobacter lowffi and Pseudomonas diminuta were isolated and identified from a thirteen-stage purification system. To evaluate the efficacy of the chemical agents used in the disinfecting process along with those used to adjust chemical characteristics of the system, over the identified bacteria, the kinetic parameter of killing time (D-value necessary to inactivate 90% of the initial bioburden (decimal reduction time was experimentally determined. Methods Pseudomonas aeruginosa, Pseudomonas fluorescens, Pseudomonas alcaligenes, Pseudomonas picketti, Flavobacterium aureum, Acinetobacter lowffi and Pseudomonas diminuta were called in house (wild bacteria. Pseudomonas diminuta ATCC 11568, Pseudomonas alcaligenes INCQS , Pseudomonas aeruginosa ATCC 15442, Pseudomonas fluorescens ATCC 3178, Pseudomonas picketti ATCC 5031, Bacillus subtilis ATCC 937 and Escherichia coli ATCC 25922 were used as 'standard' bacteria to evaluate resistance at 25°C against either 0.5% citric acid, 0.5% hydrochloric acid, 70% ethanol, 0.5% sodium bisulfite, 0.4% sodium hydroxide, 0.5% sodium hypochlorite, or a mixture of 2.2% hydrogen peroxide (H2O2 and 0.45% peracetic acid. Results The efficacy of the sanitizers varied with concentration and contact time to reduce decimal logarithmic (log10 population (n cycles. To kill 90% of the initial population (or one log10 cycle, the necessary time (D-value was for P. aeruginosa into: (i 0.5% citric acid, D = 3.8 min; (ii 0.5% hydrochloric acid, D = 6.9 min; (iii 70% ethanol, D = 9.7 min; (iv 0.5% sodium bisulfite, D = 5.3 min; (v 0.4% sodium hydroxide, D = 14.2 min; (vi 0.5% sodium

  10. Experimental study and simulation of phosphorus purification effects of bioretention systems on urban surface runoff.

    Directory of Open Access Journals (Sweden)

    Jiake Li

    Full Text Available Excessive phosphorus (P contributes to eutrophication by degrading water quality and limiting human use of water resources. Identifying economic and convenient methods to control soluble reactive phosphorus (SRP pollution in urban runoff is the key point of rainwater management strategies. Through three series of different tests involving influencing factors, continuous operation and intermittent operation, this study explored the purification effects of bioretention tanks under different experimental conditions, it included nine intermittent tests, single field continuous test with three groups of different fillers (Fly ash mixed with sand, Blast furnace slag, and Soil, and eight intermittent tests with single filler (Blast furnace slag mixed with sand. Among the three filler combinations studied, the filler with fly ash mixed with sand achieved the best pollution reduction efficiency. The setting of the submerged zone exerted minimal influence on the P removal of the three filler combinations. An extension of the dry period slightly promoted the P purification effect. The combination of fly ash mixed with sand demonstrated a positive purification effect on SRP during short- or long-term simulated rainfall duration. Blast furnace slag also presented a positive purification effect in the short term, although its continuous purification effect on SRP was poor in the long term. The purification abilities of soil in the short and long terms were weak. Under intermittent operations across different seasons, SRP removal was unstable, and effluent concentration processes were different. The purification effect of the bioretention system on SRP was predicted through partial least squares regression (PLS modeling analysis. The event mean concentration removal of SRP was positively related to the adsorption capacity of filler and rainfall interval time and negatively related to submerged zones, influent concentration and volume.

  11. Increase of COP for heat transformer in water purification systems. Part I - Increasing heat source temperature

    International Nuclear Information System (INIS)

    Siqueiros, J.; Romero, R.J.

    2007-01-01

    The integration of a water purification system in a heat transformer allows a fraction of heat obtained by the heat transformer to be recycled, increasing the heat source temperature. Consequently, the evaporator and generator temperatures are also increased. For any operating conditions, keeping the condenser and absorber temperatures and also the heat load to the evaporator and generator, a higher value of COP is obtained when only the evaporator and generator temperatures are increased. Simulation with proven software compares the performance of the modeling of an absorption heat transformer for water purification (AHTWP) operating with water/lithium bromide, as the working fluid-absorbent pair. Plots of enthalpy-based coefficients of performance (COP ET ) and the increase in the coefficient of performance (COP) are shown against absorber temperature for several thermodynamic operating conditions. The results showed that proposed (AHTWP) system is capable of increasing the original value of COP ET more than 120%, by recycling part of the energy from a water purification system. The proposed system allows to increase COP values from any experimental data for water purification or any other distillation system integrated to a heat transformer, regardless of the actual COP value and any working fluid-absorbent pair

  12. Integrated Procurement Management System, Version II

    Science.gov (United States)

    Collier, L. J.

    1985-01-01

    Integrated Procurement Management System, Version II (IPMS II) is online/ batch system for collecting developing, managing and disseminating procurementrelated data at NASA Johnson Space Center. Portions of IPMS II adaptable to other procurement situations.

  13. Field Testing of a Small Water Purification System for Non-PRASA Rural Communities

    Science.gov (United States)

    Small, rural communities typically do not have adequate water purification systems to sustain their life quality and residents are exposed to pathogens present in drinking water. In Puerto Rico (PR), approximately 4% of the population does not have access to drinking water provi...

  14. Purification of uranothorite solid solutions from polyphase systems

    Energy Technology Data Exchange (ETDEWEB)

    Clavier, Nicolas, E-mail: nicolas.clavier@icsm.fr [ICSM, UMR 5257 CEA/CNRS/UM2/ENSCM, Site de Marcoule – Bât. 426, BP 17171, 30207 Bagnols/Cèze cedex (France); Szenknect, Stéphanie; Costin, Dan Tiberiu; Mesbah, Adel; Ravaux, Johann [ICSM, UMR 5257 CEA/CNRS/UM2/ENSCM, Site de Marcoule – Bât. 426, BP 17171, 30207 Bagnols/Cèze cedex (France); Poinssot, Christophe [CEA/DEN/DRCP/DIR, Site de Marcoule – Bât. 400, BP 17171, 30207 Bagnols/Cèze cedex (France); Dacheux, Nicolas [ICSM, UMR 5257 CEA/CNRS/UM2/ENSCM, Site de Marcoule – Bât. 426, BP 17171, 30207 Bagnols/Cèze cedex (France)

    2013-10-15

    Graphical abstract: Display Omitted -- Highlights: •Purification of Th{sub 1−x}U{sub x}SiO{sub 4} uranothorites from oxide mixture was investigated. •Repetition of centrifugation steps was discarded due to poor recovery yields. •Successive washings in acid and basic media allowed the elimination of oxide secondary phases. •Structural and microstructural characterization of the purified samples was provided. -- Abstract: The mineral coffinite, nominally USiO{sub 4}, and associated Th{sub 1−x}U{sub x}SiO{sub 4} uranothorite solid solutions are of great interest from a geochemical point of view and in the case of the direct storage of spent nuclear fuels. Nevertheless, they clearly exhibit a lack in the evaluation of their thermodynamic data, mainly because of the difficulties linked with their preparation as pure phases. This paper thus presents physical and chemical methods aiming to separate uranothorite solid solutions from oxide additional phases such as amorphous SiO{sub 2} and nanometric crystallized Th{sub 1−y}U{sub y}O{sub 2}. The repetition of centrifugation steps envisaged in first place was rapidly dropped due to poor recovery yields, to the benefit of successive washings in acid then basic media. Under both static and dynamic flow rates (i.e. low or high rate of leachate renewal), ICP-AES (Inductively Coupled Plasma – Atomic Emission Spectroscopy) analyses revealed the systematic elimination of Th{sub 1−y}U{sub y}O{sub 2} in acid media and of SiO{sub 2} in basic media. Nevertheless, two successive steps were always needed to reach pure samples. On this basis, a first cycle performed in static conditions was chosen to eliminate the major part of the accessory phases while a second one, in dynamic conditions, allowed the elimination of the residual impurities. The complete purification of the samples was finally evidenced through the characterization of the samples by the means of PXRD (Powder X-Ray Diffraction), SEM (Scanning Electron

  15. Advanced Water Purification System for In Situ Resource Utilization Project

    Science.gov (United States)

    Anthony, Stephen M.

    2014-01-01

    A main goal in the field of In Situ Resource Utilization is to develop technologies that produce oxygen from regolith to provide consumables to an extratrrestrial outpost. The processes developed reduce metal oxides in the regolith to produce water, which is then electrolyzed to produce oxygen. Hydrochloric and hydrofluoric acids are byproducts of the reduction processes, which must be removed to meet electrolysis purity standards. We previously characterized Nation, a highly water selective polymeric proton-exchange membrane, as a filtrtion material to recover pure water from the contaminated solution. While the membranes successfully removed both acid contaminants, the removal efficiency of and water flow rate through the membranes were not sufficient to produce large volumes of electrolysis-grade water. In the present study, we investigated electrodialysis as a potential acid removable technique. Our studies have show a rapid and significant reduction in chloride and fluoride concentrations in the feed solution, while generating a relatively small volume of concentrated waste water. Electrodialysis has shown significant promise as the primary separation technique in ISRU water purification processes.

  16. Development of a transfer model for design of sodium purification systems for Fast Breeder Reactors

    International Nuclear Information System (INIS)

    Khatcheressian, N.

    2013-01-01

    Operating a Sodium Fast Reactor (SFR) in reliable and safe conditions requires to master the quality of the sodium fluid coolant, regarding oxygen and hydrogen impurities contents. A cold trap is a purification unit in SFR, designed for maintaining oxygen and hydrogen contents within acceptable limits. The purification of these impurities is based on crystallization of sodium hydride on cold walls and sodium oxide or hydride on wire mesh packing. Indeed, as oxygen and hydrogen solubilities are nearly nil at temperatures close to the sodium fusion point, i.e. 97.8 C, on line sodium purification can be performed by crystallization of sodium oxide and hydride from liquid sodium flows. However, the management of cold trap performances is necessary to prevent from unforeseen maintenance operations, which could induce shut-down of the reactor. It is thus essential to understand how a cold trap fills up with impurities crystallization in order to optimize the design of this system and to overcome any problems during nominal operation. The objective is to develop a design and simulation tool for cold traps able to predict the location and the amount of the impurities deposited. Crystallization model involve phenomena coupling in a porous medium with hydrodynamics, heat and mass transfer, distinguishing nucleation and growth phases for each impurity. It enables to understand how thermo hydraulic conditions and growing impurities interact on each other. This analysis will adapt operating and management conditions in order to optimize purification requirements. (author) [fr

  17. Combined cooling and purification system for nuclear reactor spent fuel pit, refueling cavity, and refueling water storage tank

    Science.gov (United States)

    Corletti, Michael M.; Lau, Louis K.; Schulz, Terry L.

    1993-01-01

    The spent fuel pit of a pressured water reactor (PWR) nuclear power plant has sufficient coolant capacity that a safety rated cooling system is not required. A non-safety rated combined cooling and purification system with redundant branches selectively provides simultaneously cooling and purification for the spent fuel pit, the refueling cavity, and the refueling water storage tank, and transfers coolant from the refueling water storage tank to the refueling cavity without it passing through the reactor core. Skimmers on the suction piping of the combined cooling and purification system eliminate the need for separate skimmer circuits with dedicated pumps.

  18. Evaluation of RSG-GAS purification system and pool warm water layer supplier performance

    International Nuclear Information System (INIS)

    Sudiyono; Suhadi; Diah-Erlina-Lestari

    2005-01-01

    Function of RSG-GAS purification system and warm water supplier (KBE 02) are to pick up dissolve activation result and another dirts of warm water layer. To keep quality of water at the decided level. The system is equipped by heater to supply warm water layer on the reactor pool surface the distribution is to reduce radiation level in the operation hall area a speciality on the reactor pool surface. Line KBE 02 tomord beam tube headitty system supplies water necessary to be shielding to beam tube in use off time. Of the RSG-GAS purification system and pool warm water layer performance date can be shown north of water is always in good condition. To require the dechded requirement. Resin live time is two years and then months

  19. Purification Performance and Production of a Re-circulating Pond Aquaculture System Based on Paddy Field

    OpenAIRE

    Gu Li; Shi-yang Zhang; Ling Tao; Xiao-li Li; Jing-hua Song; Chun-xue Zhang; Jian-qiang Zhu

    2012-01-01

    Developing improved aquaculture systems with a more efficient use of water and less environmental impact is becoming a crying need. A re-circulating aquaculture system consisting of paddy field and fish pond is a new culture mode due to aquaculture combing with agriculture. The present study focused on the purification capacity of the paddy field on nitrogen, phosphorus and organic matter, the fluctuation trend of water quality conditions during the whole rearing process and the culture effic...

  20. Pepsinogens I and II: purification from gastric mucosa and radioimmunoassay in serum

    International Nuclear Information System (INIS)

    Samloff, I.M.

    1982-01-01

    Pepsinogen I and pepsinogen II were purified from gastric mucosa and used to develop a radioimmunoassay for pepsinogen II and an improved radioimmunoassay for pepsinogen I. Each immunochemically homogeneous preparation contained only its characteristic components by radioelectophoretic analysis, and migrated as a singly band in polyacrylamide gel. The mean (+/- SD) level of serum pepsinogen II in 42 healthy control subjects was 10,8 +/- 3.8 ng/ml, significantly less (p less than 0.001) than the level of pepsinogen I, which was 62.9 +/- 22.2 ng/ml. The correlation between serum pepsinogen I and pepsinogen II was highly significant (r . 0.700, p less than 0.001) in these subjects. In 20 patients with pernicious anemia the mean serum pepsinogen II level was 10.6 +/- 2.5 ng/ml, not different from normal, but significantly higher (p less than 0.001) than the level of pepsinogen I which was 5.9 +/- 4.7 ng/ml. IN 10 patients with total gastrectomy, serum pepsinogen I was 3.9 +/- 3.1 ng/ml and serum pepsinogen II was 3.2 +/- 3.1 ng/ml; both values were significantly lower (p less than 0.001) than the corresponding levels in pernicious anemia. The predominance of pepsinogen I in the serum of healthy control subjects suggests that either the gastric chief cell normally releases more pepsinogen I than pepsinogen II into the circulation or that pepsinogen I has longer metabolic clearance rate than pepsinogen II. The marked decrease in serum pepsinogen I in patients with pernicious anemia is best explained by a loss of gastric chief cells due to severe atropic gastritis of the fundic glands. The normal distribution of serum pepsinogen II levels in these patients may reflect an increased number of pyloric glands due to pyloric gland metaplasia of the proximal stomach

  1. High capacity argon extraction and purification system. [Suitable for age estimation of rocks

    Energy Technology Data Exchange (ETDEWEB)

    Balogh, K; Morik, Gy [Magyar Tudomanyos Akademia Atommag Kutato Intezete, Debrecen

    1979-01-01

    A high capacity metal-glass argon extraction and purification system has been developed for K/Ar dating of geologic materials. A set of samples can be loaded simultaneously and degassed in turn in the system by high frequency induction heating in a molybdenum crucible. The argon purification is carried out by titanium sponge, molecular sieve, copper oxide and glass and charcoal filled traps cooled by liquid nitrogen. The /sup 38/Ar spike and the atmospheric argon used for calibrating the mass spectrometer are dispensed by a gas-pipette system. 80-120 minutes after starting the degassing of the sample, the purified argon can be introduced into the mass spectrometer; the gettering materials regenerate in 1-5 hours, thus 1-3 samples may be analysed a day. The atmospheric argon inflow during an experimental process is less than 5x10/sup -8/ cc STP.

  2. CAREM 25: Design of resin bed for purification and boron removal systems

    International Nuclear Information System (INIS)

    Chocron, Mauricio; Iglesias, Alberto M.; Jimenez Rebagliati, Raul; Raffo Calderon, Maria C.; La Gamma, Ana M.

    2000-01-01

    The purification of the water the primary coolant of a water cooled nuclear reactor as well as the water of many auxiliary systems is controlled by the use of ion exchange resins. In the present paper, the resin beds for three different systems are specified: the purification and control volume system, the suppression pool water and the spent fuel pool water for the reactor CAREM-25. In all cases the dimensioning calculations have been done taking in consideration the amount of contaminants and corrosion products generated under normal operation or post-accident. Also, the results have been contrasted with the experience of the nuclear power plants in operation in Argentina, international design criteria and international standards. For the primary coolant, the boron-removal beds have been sized and an estimation of the maximum dose received by the resins have been calculated. It have been found that the result is well below the damaging threshold reported in the literature. (author)

  3. Purification, crystallization and preliminary X-ray diffraction analysis of the glyoxalase II from Leishmania infantum

    International Nuclear Information System (INIS)

    Trincão, José; Sousa Silva, Marta; Barata, Lídia; Bonifácio, Cecília; Carvalho, Sandra; Tomás, Ana Maria; Ferreira, António E. N.; Cordeiro, Carlos; Ponces Freire, Ana; Romão, Maria João

    2006-01-01

    A glyoxalase II from L. infantum was cloned, purified and crystallized and its structure was solved by X-ray crystallography. In trypanosomatids, trypanothione replaces glutathione in all glutathione-dependent processes. Of the two enzymes involved in the glyoxalase pathway, glyoxalase I and glyoxalase II, the latter shows absolute specificity towards trypanothione thioester, making this enzyme an excellent model to understand the molecular basis of trypanothione binding. Cloned glyoxalase II from Leishmania infantum was overexpressed in Escherichia coli, purified and crystallized. Crystals belong to space group C222 1 (unit-cell parameters a = 65.6, b = 88.3, c = 85.2 Å) and diffract beyond 2.15 Å using synchrotron radiation. The structure was solved by molecular replacement using the human glyoxalase II structure as a search model. These results, together with future detailed kinetic characterization using lactoyltrypanothione, should shed light on the evolutionary selection of trypanothione instead of glutathione by trypano-somatids

  4. Development of a purification system at Dhruva to treat oil contaminated and chemically impure heavy water

    International Nuclear Information System (INIS)

    Suttraway, S.K.; Mishra, V.; Bitla, S.V.; Ghosh, S.K.

    2006-01-01

    Dhruva, a 100 MW (thermal) Research reactor uses Heavy Water as moderator, reflector and coolant. Normally during plant operation, the Heavy water from the system gets removed during operational and maintenance activities and this collected heavy water gets degraded and contaminated in the process. The degraded heavy water meeting the chemical specification requirement of the up gradation plant is sent for up gradation. Part of the Heavy water collected is contaminated with various organic and inorganic impurities and therefore cannot be sent for IP up gradation as it does not meet the chemical specification of the up gradation plant. This contaminated Heavy water was being stored in SS drums. Over the years of Reactor operation reasonable amount of contaminated Heavy water got collected in the plant. This Heavy water collected from leakages, during routine maintenance, operational activities and fuelling operation had tritium activity and variety of contamination including oil, chlorides, turbidity due to which the specific conductivity was very high. It was decided to purify this Heavy water in house to bring it up to up gradation plant chemical specification requirement. There were number of challenges in formulating a scheme to purify this Heavy water. The scheme needed to be simple and compact in design which could be set up in the plant itself. It should not pose radiological hazards due to radioactive Heavy water during its purification and handling. The contaminated Heavy water collected in drums had varying chemistry and IP. The purification plant should be able to do batch processing so that the different IP and chemical quality of Heavy water stored in different drums are not mixed during purification. It should be capable of removing the oil, chlorides, turbidity and decrease the conductivity to acceptable limits of the Up gradation plant. A purification plant was developed and commissioned after detail laboratory studies and trials. This paper explains

  5. Aqueous two-phase system purification for superoxide dismutase induced by menadione from Phanerochaete chrysosporium.

    Science.gov (United States)

    Kavakcıoğlu, Berna; Tongul, Burcu; Tarhan, Leman

    2017-03-01

    In the present work, the partitioning behavior of menadione-induced superoxide dismutase (SOD; EC 1.15.1.1), an antioxidant enzyme that has various applications in the medical and cosmetic industries, from the white rot fungus Phanerochaete chrysosporium has been characterized on different types of aqueous two-phase systems (ATPSs) (poly(ethylene glycol)/polypropylene glycol (PEG/PPG)-dextran, PEG-salt and PPG-salt). PEG-salt combinations were found most optimal systems for the purification of SOD. The best partition conditions were found using the PEG-3350 24% and K 2 HPO 4 5% (w/w) with pH 7.0 at 25 °C. The partition coefficient of total SOD activity and total protein concentration observed in this system were 0.17 and 6.65, respectively, with the recovery percentage as 78.90% in the bottom phase and 13.17% in the top phase. The highest purification fold for SOD from P. chrysosporium was found as 6.04 in the bottom phase of PEG 3350%24 - K 2 HPO 4 %5 (w/w) system with pH 7.0. SOD purified from P. chrysosporium was determined to be a homodimer in its native state with a molecular weight of 60  ± 4 kDa. Consequently, simple and only one step PEG-salt ATPS system was developed for SOD purification from P. chrysosporium.

  6. MPS II drift chamber system

    International Nuclear Information System (INIS)

    Platner, E.D.

    1982-01-01

    The MPS II detectors are narrow drift space chambers designed for high position resolution in a magnetic field and in a very high particle flux environment. Central to this implementation was the development of 3 multi-channel custom IC's and one multi-channel hybrid. The system is deadtimeless and requires no corrections on an anode-to-anode basis. Operational experience and relevance to ISABELLE detectors is discussed

  7. Dipeptidyl peptidase-II from probiotic Pediococcus acidilactici: Purification and functional characterization.

    Science.gov (United States)

    Gandhi, Dimpi; Chanalia, Preeti; Attri, Pooja; Dhanda, Suman

    2016-12-01

    Dipeptidylpeptidase-II (DPP-II, E.C. 3.4.14.2), an exopeptidase was purified 15.4 fold with specific activity and yield of 15.4U/mg/mL and 14.68% respectively by a simple two step procedure from a probiotic Pediococcus acidilactici. DPP-II is 38.7KDa homodimeric serine peptidase with involvement of His and subunit mass of 18.9KDa. The enzyme exhibited optimal activity at pH 7.0 and 37°C with activation energy of 24.97kJ/mol. The enzyme retained more than 90% activity upto 50°C thus adding industrial importance. DPP-II hydrolysed Lys-Ala-4mβNA with K M of 50μM and V max of 30.8nmol/mL/min. In-silico characterization studies of DPP-II on the basis of peptide fragments obtained by MALDI-TOF revealed an evolutionary relationship between DPP-II of prokaryotes and phosphate binding proteins. Secondary and three-dimensional structure of enzyme was also deduced by in-silico approach. Functional studies of DPP-II by TLC and HPLC-analysis of collagen degraded products revealed that enzyme action released free amino acids and other metabolites. Microscopic and SDS-PAGE analysis of enzyme treated analysis of chicken's chest muscle (meat) hydrolysis revealed change and hydrolysis of myofibrils. This may affect the flavor and texture of meat thereby suggesting its role in meat tenderization. Being a protein of LAB (Lactic acid bacteria), it is also expected to be safe. Copyright © 2016 Elsevier B.V. All rights reserved.

  8. Guide to the use of pond systems in South Africa for the purification of raw and partially treated sewage

    CSIR Research Space (South Africa)

    Meiring, PGJ

    1968-01-01

    Full Text Available Some of the effluents from the sewage purification systems covered by this report show quite marked seasonal variations. No assurance can be given that the effluents would meet promulgated standards from season to season. The effluent from...

  9. Two novel solvent system compositions for protected synthetic peptide purification by centrifugal partition chromatography.

    Science.gov (United States)

    Amarouche, Nassima; Giraud, Matthieu; Forni, Luciano; Butte, Alessandro; Edwards, F; Borie, Nicolas; Renault, Jean-Hugues

    2014-04-11

    Protected synthetic peptide intermediates are often hydrophobic and not soluble in most common solvents. They are thus difficult to purify by preparative reversed-phase high-performance liquid chromatography (RP-HPLC), usually used for industrial production. It is then challenging to develop alternative chromatographic purification processes. Support-free liquid-liquid chromatographic techniques, including both hydrostatic (centrifugal partition chromatography or CPC) and hydrodynamic (counter-current chromatography or CCC) devices, are mainly involved in phytochemical studies but have also been applied to synthetic peptide purification. In this framework, two new biphasic solvent system compositions covering a wide range of polarity were developed to overcome solubility problems mentioned above. The new systems composed of heptane/tetrahydrofuran/acetonitrile/dimethylsulfoxide/water and heptane/methyl-tetrahydrofuran/N-methylpyrrolidone/water were efficiently used for the CPC purification of a 39-mer protected exenatide (Byetta®) and a 8-mer protected peptide intermediate of bivalirudin (Angiox®) synthesis. Phase compositions of the different biphasic solvent systems were determined by (1)H nuclear magnetic resonance. Physico-chemical properties including viscosity, density and interfacial tension of these biphasic systems are also described. Copyright © 2014 Elsevier B.V. All rights reserved.

  10. Numerical simulation of Venturi ejector reactor in yellow phosphorus purification system

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Xiao-jing; Tang, Lei, E-mail: alanleyfly@gmail.com; Jiang, Zeng

    2014-03-15

    Highlights: • Venturi ejector reactor is used in yellow phosphorus purification system to obtain high purity phosphorus. • We study the changes of vacuum region and the performances of Venturi ejector reactor with different operating pressure. • The whole study is aim to investigate the operating conditions, rather than to find out the small details of the chemical reaction. - Abstract: A novel type of Venturi ejector reactor, which was used in a pilot plant test in a factory in Guizhou in China, was developed to overcome the insufficiency of chemical reaction in the stirred-tank reactor in yellow phosphorus purification system. The effects of different working medium, the changes of vacuum region, and the performances of the Venturi ejector reactor with different operating pressure were investigated by FLUENT. Results show that the absolute value of vacuum pressure of single-phase flow was smaller than two-phase flow at the same operating conditions, which meat two-phase flow has a higher suction capability. Reflow phenomena occurred near the exit of suction pipe and nozzle. The former reflow which leads to energy loss of vacuum region was undesirable, and the latter was beneficial to the dispersion of liquid yellow phosphorus. With a flow rate ratio below 0.45, the performance of the Venturi ejector reactor was effective. By adjusting the operating pressure, a proper flow rate ratio could be satisfied to meet the production needs in yellow phosphorus purification system.

  11. Moderate temperature gas purification system: Application to high calorific coal-derived fuel

    Energy Technology Data Exchange (ETDEWEB)

    Kobayashi, M.; Shirai, H.; Nunokawa, M. [Central Research Institute of Electric Power Industry, Kanagawa (Japan)

    2008-01-15

    Simultaneous removal of dust, alkaline and alkaline-earth metals, halides and sulfur compounds is required to enlarge application of coal-derived gas to the high-temperature fuel cells and the fuel synthesis through chemical processing. Because high calorific fuel gas, such as oxygen-blown coal gas, has high carbon monoxide content, high-temperature (above 450{sup o}C) gas purification system is always subjected to the carbon deposition. We suggest moderate temperature (around 300{sup o}C) operation of the gas purification system to avoid the harmful disproportionation reaction and efficient removal of the various contaminants. Because the reaction rate is predominant to the performance of contaminant removal in the moderate temperature gas purification system, we evaluated the chemical removal processes; performance of the removal processes for halides and sulfur compounds was experimentally evaluated. The halide removal process with sodium aluminate sorbent had potential performance at around 300{sup o}C. The sulfur removal process with zinc ferrite sorbent was also applicable to the temperature range, though the reaction kinetics of the sorbent is essential to be approved.

  12. Moderate temperature gas purification system: application to high calorific coal derived fuel

    Energy Technology Data Exchange (ETDEWEB)

    M. Kobayashi; H. Shirai; M. Nunokawa [Central Research Institute of Electric Power Industry (CRIEPI), Kanagawa (Japan)

    2005-07-01

    Simultaneous removal of dust, alkaline and alkaline-earth metals, halides and sulfur compounds is required to enlarge application of coal-derived gas to the high temperature fuel cells and the fuel synthesis through chemical processing. Because high calorific fuel gas, such as oxygen-blown coal gas, has high carbon monoxide content, high temperature gas purification system is always subjected to the carbon deposition and slippage of contaminant of high vapor pressure. It was suggested that moderate temperature operation of the gas purification system is applied to avoid the harmful disproportionation reaction and efficient removal of the various contaminants. To establish the moderate temperature gas purification system, the chemical-removal processes where the reaction rate is predominant to the performance of contaminant removal should be evaluated. Performance of the removal processes for halides and sulfur compounds were experimentally evaluated. The halide removal process with sodium based sorbent had potential good performance at around 300{sup o}C. The sulfur removal process was also applicable to the temperature range, although the improvement of the sulfidation reaction rate is considered to be essential. 11 refs., 8 figs., 1 tab.

  13. A simple high efficiency solar water purification system

    Energy Technology Data Exchange (ETDEWEB)

    Duff, W.S.; Hodgson, D.A. [Colorado State University, Fort Collins, CO (United States). Dept. of Mechanical Engineering

    2005-07-01

    A new passive solar water pasteurization system based on density difference flow principles has been designed, built and tested. The system contains no valves and regulates flow based on the density difference between two columns of water. The new system eliminates boiling problems encountered in previous designs. Boiling is undesirable because it may contaminate treated water. The system with a total absorber area of 0.45 m2 has achieved a peak flow rate of 19.3 kg/h of treated water. Experiments with the prototype systems presented in this paper show that density driven systems are an attractive option to existing solar water pasteurization approaches. (author)

  14. Final LDRD report :ultraviolet water purification systems for rural environments and mobile applications.

    Energy Technology Data Exchange (ETDEWEB)

    Banas, Michael Anthony; Crawford, Mary Hagerott; Ruby, Douglas Scott; Ross, Michael P.; Nelson, Jeffrey Scott; Allerman, Andrew Alan; Boucher, Ray

    2005-11-01

    We present the results of a one year LDRD program that has focused on evaluating the use of newly developed deep ultraviolet LEDs in water purification. We describe our development efforts that have produced an LED-based water exposure set-up and enumerate the advances that have been made in deep UV LED performance throughout the project. The results of E. coli inactivation with 270-295 nm LEDs are presented along with an assessment of the potential for applying deep ultraviolet LED-based water purification to mobile point-of-use applications as well as to rural and international environments where the benefits of photovoltaic-powered systems can be realized.

  15. Purification and H-1 NMR spectroscopic characterization of phase II metabolites of tolfenamic acid

    DEFF Research Database (Denmark)

    Sidelmann, U. G.; Christiansen, E.; Krogh, L.

    1997-01-01

    samples obtained on days 7 to 10 from a human volunteer after oral administration of 200 mg of the drug three times per day (steady-state plasma concentration). The metabolites of tolfenamic acid were initially concentrated by preparative solid phase extraction (PSPE) chromatography, thereby removing...... the endogenous polar compounds that are present in the urine. The individual metabolites were purified by preparative high performance liquid chromatography (HPLC) and then identified using H-1 NMR, Both one- and two-dimensional NMR experiments were performed to identify the phase II metabolites of tolfenamic......), and N-(2-methyl-4-hydroxyphenyl)-anthranilic acid (11) were identified. The phase II metabolites (5-11) had not previously been identified in urine from humans administered tolfenamic acid. The phase I metabolites of the glucuronides 7, 8, 10, and 11 were identified here for the first time. An HPLC...

  16. Isolation and Purification of Complex II from Proteus Mirabilis Strain ATCC 29245

    Science.gov (United States)

    Shabbiri, Khadija; Ahmad, Waqar; Syed, Quratulain; Adnan, Ahmad

    2010-01-01

    A respiratory complex was isolated from plasma membrane of pathogenic Proteus mirabilis strain ATCC 29245. It was identified as complex II consisting of succinate:quinone oxidoreductase (EC 1.3.5.1) containing single heme b. The complex II was purified by ion-exchange chromatography and gel filtration. The molecular weight of purified complex was 116.5 kDa and it was composed of three subunits with molecular weights of 19 kDa, 29 kDa and 68.5 kDa. The complex II contained 9.5 nmoles of cytochrome b per mg protein. Heme staining indicated that the 19 kDa subunit was cytochrome b. Its reduced form showed absorptions peaks at 557.0, 524.8 and 424.4 nm. The α-band was shifted from 557.0 nm to 556.8 nm in pyridine ferrohemochrome spectrum. The succinate: quinone oxidoreductase activity was found to be high in this microorganism. PMID:24031557

  17. Biofilm bacterial communities in urban drinking water distribution systems transporting waters with different purification strategies.

    Science.gov (United States)

    Wu, Huiting; Zhang, Jingxu; Mi, Zilong; Xie, Shuguang; Chen, Chao; Zhang, Xiaojian

    2015-02-01

    Biofilm formation in drinking water distribution systems (DWDS) has many adverse consequences. Knowledge of microbial community structure of DWDS biofilm can aid in the design of an effective control strategy. However, biofilm bacterial community in real DWDS and the impact of drinking water purification strategy remain unclear. The present study investigated the composition and diversity of biofilm bacterial community in real DWDSs transporting waters with different purification strategies (conventional treatment and integrated treatment). High-throughput Illumina MiSeq sequencing analysis illustrated a large shift in the diversity and structure of biofilm bacterial community in real DWDS. Proteobacteria, Firmicutes, Bacteroidetes, Actinobacteria, Nitrospirae, and Cyanobacteria were the major components of biofilm bacterial community. Proteobacteria (mainly Alphaproteobacteria, Betaproteobacteria, and Gammaproteobacteria) predominated in each DWDS biofilm, but the compositions of the dominant proteobacterial classes and genera and their proportions varied among biofilm samples. Drinking water purification strategy could shape DWDS biofilm bacterial community. Moreover, Pearson's correlation analysis indicated that Actinobacteria was positively correlated with the levels of total alkalinity and dissolved organic carbon in tap water, while Firmicutes had a significant positive correlation with nitrite nitrogen.

  18. Highly Efficient, Solid State Hydrogen Purification for Resource Recovery, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — Long duration manned space exploration requires further closure of the oxygen loop of the life support system than is currently realized aboard the International...

  19. Operation and maintenance techniques of pool and pool water purification system in IMEF

    Energy Technology Data Exchange (ETDEWEB)

    Soong, Woong Sup

    1999-03-01

    IMEF pool is used pass way between pool and hot cell in order to inlet and outlet of fuel pin in cask. All operation is performed conforming with naked eyes. Therefore floating matter is filtered so as to easy under water handling. Also radioactivity in pool water is controlled according to the nuclear law, radioactivity ration maintained less than 15mR/hr on pool side. Perfect operation and maintenance can be achieved well trained operator. Result obtained from the perfection can give more influence over restrain, spreading contamination of radioactivity materials. This report describes operation and maintenance technique of pool water purification system in IMEF. (Author). 7 refs., 13 figs.

  20. Operation and maintenance techniques of pool and pool water purification system in IMEF

    International Nuclear Information System (INIS)

    Soong, Woong Sup

    1999-03-01

    IMEF pool is used pass way between pool and hot cell in order to inlet and outlet of fuel pin in cask. All operation is performed conforming with naked eyes. Therefore floating matter is filtered so as to easy under water handling. Also radioactivity in pool water is controlled according to the nuclear law, radioactivity ration maintained less than 15mR/hr on pool side. Perfect operation and maintenance can be achieved well trained operator. Result obtained from the perfection can give more influence over restrain, spreading contamination of radioactivity materials. This report describes operation and maintenance technique of pool water purification system in IMEF. (Author). 7 refs., 13 figs

  1. Conceptual design of primary coolant purification system using cylindrical membrane for nuclear energy system base on HTGR

    International Nuclear Information System (INIS)

    Piping Supriatna

    2011-01-01

    The recent progress of reactor technology design for next generation reactor will be implemented on cogeneration reactor, which the aim of reactor operation not only for generating electrical energy, but also for other application like desalination, industrial manufacturing process, hydrogen production, Enhanced Oil Recovery (EOR), etc. The cogeneration reactor concept developed for generate energy effectively, efficiently and sustainable, which reserve of uranium and thorium nuclear fuel for cogeneration reactor is supply able for world energy demand until next thousand years. The cogeneration reactor produce temperature output higher than commonly Nuclear Power Plant (NPP), and need special Heat Exchanger with helium gas as coolant. In order to preserve heat transfer with high efficiency, constant purity of the gas must be maintained as well as possible, especially contamination from its impurities. In this research has been designed modeling and assessment of primary coolant gas purification system with purify and fill up helium gas continuously, by using Cylindrical Helium Splitting Membrane and helium gas inventory system. The result of flow rate helium assessment for the purification system is 0.844x10 -3 kg/sec, where helium flow rate of reactor primary coolant is 120 kg/sec. The result of study show that the Primary Coolant Gas Purification System is enable to be implemented on Cogeneration Reactor HTGR200C. (author)

  2. Hydrogen Purification and Recycling for an Integrated Oxygen Recovery System Architecture

    Science.gov (United States)

    Abney, Morgan B.; Greenwood, Zachary; Wall, Terry; Miller, Lee; Wheeler, Ray

    2016-01-01

    The United States Atmosphere Revitalization life support system on the International Space Station (ISS) performs several services for the crew including oxygen generation, trace contaminant control, carbon dioxide (CO2) removal, and oxygen recovery. Oxygen recovery is performed using a Sabatier reactor developed by Hamilton Sundstrand, wherein CO2 is reduced with hydrogen in a catalytic reactor to produce methane and water. The water product is purified in the Water Purification Assembly and recycled to the Oxygen Generation Assembly (OGA) to provide O2 to the crew. This architecture results in a theoretical maximum oxygen recovery from CO2 of approximately 54% due to the loss of reactant hydrogen in Sabatier-produced methane that is currently vented outside of ISS. Plasma Methane Pyrolysis technology (PPA), developed by Umpqua Research Company, provides the capability to further close the Atmosphere Revitalization oxygen loop by recovering hydrogen from Sabatier-produced methane. A key aspect of this technology approach is to purify the hydrogen from the PPA product stream which includes acetylene, unreacted methane and byproduct water and carbon monoxide. In 2015, four sub-scale hydrogen separation systems were delivered to NASA for evaluation. These included two electrolysis single-cell hydrogen purification cell stacks developed by Sustainable Innovations, LLC, a sorbent-based hydrogen purification unit using microwave power for sorbent regeneration developed by Umpqua Research Company, and a LaNi4.6Sn0.4 metal hydride produced by Hydrogen Consultants, Inc. Here we report the results of these evaluations, discuss potential architecture options, and propose future work.

  3. Design, Prototyping, and Assessment of a Wastewater Closed-Loop Recovery and Purification System

    Directory of Open Access Journals (Sweden)

    Marco Bortolini

    2017-10-01

    Full Text Available Efforts to decrease the water use within industry are mandatory to pursue product and process sustainability. Particularly, the European Union (EU is at the top level for water consumption in industry, while some sectors, such as the food and beverage (F&B, are highly water-intensive with hundreds of liters per hour of consumed and, then, drained water. This article provides a systematic overview of the most innovative insights coming from an EU Eco-Innovation project dealing with greening the F&B industry through the design, prototyping, technical, economic, and environmental assessment of a wastewater closed-loop recovery and purification system. The system, tailored for a standard mid-size F&B company using 2–3 billion L/year of raw water, collects, purifies and recirculates the key produced wastewater streams with an overall recovery efficiency of about 56%. The proposed purification technology comes from the most efficient combination of membrane-based filtration methods, reverse osmosis (RO, and ultraviolet modules. Evidence from the technical design, full-scale on-site technology prototyping, net-present-value (NPV analysis and system life-cycle-assessment (LCA are presented concluding about the convenience of adopting the proposed solution to reduce costs and impacts on the environment.

  4. An Improved CO2 Separation and Purification System Based on Cryogenic Separation and Distillation Theory

    Directory of Open Access Journals (Sweden)

    Gang Xu

    2014-05-01

    Full Text Available In this study, an improved CO2 separation and purification system is proposed based on in-depth analyses of cryogenic separation and distillation theory as well as the phase transition characteristics of gas mixtures containing CO2. Multi-stage compression, refrigeration, and separation are adopted to separate the majority of the CO2 from the gas mixture with relatively low energy penalty and high purity. Subsequently, the separated crude liquid CO2 is distilled under high pressure and near ambient temperature conditions so that low energy penalty purification is achieved. Simulation results indicate that the specific energy consumption for CO2 capture is only 0.425 MJ/kgCO2 with 99.9% CO2 purity for the product. Techno-economic analysis shows that the total plant investment is relatively low. Given its technical maturity and great potential in large-scale production, compared to conventional MEA and SelexolTM absorption methods, the cost of CO2 capture of the proposed system is reduced by 57.2% and 45.9%, respectively. The result of this study can serve as a novel approach to recovering CO2 from high CO2 concentration gas mixtures.

  5. Overview of the SECOM II communications system

    International Nuclear Information System (INIS)

    Olson, W.D.

    1977-04-01

    The conversion of the SECOM system to the SECOM II configuration has been completed and all communications between the central control station and vehicles carrying nuclear weapons and special nuclear materials are being handled by the SECOM II system. A summary of the system characteristics and the improvements achieved over the all-voice system is shown. Through the combined efforts of ALO and Sandia personnel, the transition from SECOM to SECOM II was achieved with a minimum disruption to the operation of the ERDA transportation system

  6. Effect of charcoal on water purification

    OpenAIRE

    Suzuki, Hirotaka; Kawahigashi, Tatsuo

    2014-01-01

    [Abstract] A natural basin system purifies water through self-purification, but the water pollution load of a river might exceed its self-purification capacity. Charcoal, which is used for other uses aside from heating, such as air purification, was evaluated experimentally for water quality purification. The experiment described herein is based on simple water quality measurements. Some experimentally obtained results are discussed.

  7. NSLS-II Radio Frequency Systems

    Energy Technology Data Exchange (ETDEWEB)

    Rose J.; Gao F.; Goel, A.; Holub, B.; Kulpin, J.; Marques, C.; Yeddulla, M.

    2015-05-03

    The National Synchrotron Light Source II is a 3 GeV X-ray user facility commissioned in 2014. The NSLS-II RF system consists of the master oscillator, digital low level RF controllers, linac, booster and storage ring RF sub-systems, as well as a supporting cryogenic system. Here we will report on RF commissioning and early operation experience of the system.

  8. Oxygen control systems and impurity purification in LBE: Learning from DEMETRA project

    Energy Technology Data Exchange (ETDEWEB)

    Brissonneau, L., E-mail: laurent.brissonneau@cea.fr [CEA/DEN, Cadarache, DTN/STPA/LIPC, F-13108 Saint-Paul-lez-Durance (France); Beauchamp, F.; Morier, O. [CEA/DEN, Cadarache, DTN/STPA/LIPC, F-13108 Saint-Paul-lez-Durance (France); Schroer, C.; Konys, J. [Karlsruher Institut fuer Technologie (KIT), Institut fuer Materialforschung III, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen (Germany); Kobzova, A.; Di Gabriele, F. [NRI, UJV Husinec-Rez 130, Rez 25068 (Czech Republic); Courouau, J.-L. [CEA/DEN, Saclay, DPC/SCCME/LECNA, F-919191 Gif-sur-Yvette (France)

    2011-08-31

    Operating a system using Lead-Bismuth Eutectic (LBE) requires a control of the dissolved oxygen concentration to avoid corrosion of structural materials and oxide build-up in the coolant. Reliable devices are therefore needed to monitor and adjust the oxygen concentration and to remove impurities during operation. In this article, we describe the learning gained from experiments run in the framework of the DEMETRA project (IP-EUROTRANS 6th FP contract) on the oxygen supply in LBE and on impurity filtration and management in different European facilities. An oxygen control device should supply oxygen in LBE at sufficient rate to compensate loss by surface oxidation, otherwise local dissolution of oxide layers might lead to the loss of steel protection against dissolution. Oxygen can be supplied by gas phase H{sub 2}O or O{sub 2}, or by solid phase, PbO dissolution. Each of these systems has substantial advantages and drawbacks. Considerations are given on devices for large scale facilities. The management of impurities (lead oxides and corrosion products) is also a crucial issue as their presence in the liquid phase or in the aerosols is likely to impair the facility, instrumentation and mechanical devices. To avoid impurity build-up on the long-term, purification of LBE is required to keep the impurity inventory low by trapping oxide and metallic impurities in specific filter units. On the basis of impurities characterisation and experimental results gained through filtration tests in different loops, this paper gives a description of the state-of-art knowledge of LBE purification with different filter media. It is now understood that the nature and behaviour of impurities formed in LBE will change according to the operating modes as well as the method to propose to remove impurities. This experience can be used to validate the basis filtration process, define the operating procedures and evaluate perspectives for the design of purification units for long

  9. Development of concept for concurrent biocide generation and water system purification. [with application to Skylab water tanks

    Science.gov (United States)

    1974-01-01

    An attempt was made to construct an electrochemical system, using iodine, for water purification in Skylab. Data cover measurements of iodine production rates, effect of electrode size and geometry on iodine production rates, and feasibility of using stainless steels as reference electrodes.

  10. Cloning, expression, and purification of recombinant protein MPT-64 from a virulent strain of Mycobacterium bovis in a prokaryotic system

    Directory of Open Access Journals (Sweden)

    Maryam Mohammadi Tashakkori

    2016-01-01

    Conclusion: These results indicated successful expression and purification of recombinant MPT-64 protein in a prokaryotic system. This protein can be used for serological diagnosis, improved detection of pathogenicity and non-pathogenicity between infected cattle, and for verification of suspected cases of bovine TB.

  11. Application of mobile blood purification system in the treatment of acute renal failure dog model in the field environment

    Directory of Open Access Journals (Sweden)

    Zhi-min ZHANG

    2014-01-01

    Full Text Available Objective To evaluate the stability, safety and efficacy of mobile blood purification system in the treatment of acute renal failure dog model in the field environment. Methods The acute renal failure model was established in 4 dogs by bilateral nephrectomy, which was thereafter treated with the mobile blood purification system. The evaluation of functional index of the mobile blood purification system was performed after a short-time (2 hours and conventional (4 hours dialysis treatment. Results The mobile blood purification system ran stably in the field environment at a blood flow of 150-180ml/min, a dialysate velocity of 2000ml/h, a replacement fluid velocity of 2000ml/h, and ultrafiltration rate of 100-200ml/h. All the functions of alarming system worked well, including static upper limit alarm of ultrafiltration pressure (>100 mmHg, upper limit alarm of ambulatory arterial pressure (>400mmHg, upper limit alarm of ambulatory venous pressure (>400mmHg, bubble alarm of vascular access, bubble alarm during the infusion of solutions, pressure alarm at the substitution pump segment and blood leaking alarm. The vital signs of the 4 dogs with acute renal failure kept stable during the treatment. After the treatment, a remarkable decrease was seen in the levels of serum urea nitrogen, creatinine and serum potassium (P0.05. Conclusions The mobile blood purification system runs normally even in a field environment. It is a flexible and portable device with a great performance in safety and stability in the treatment of acute renal failure. DOI: 10.11855/j.issn.0577-7402.2013.12.15

  12. Study on Purification Diatomite with nitric acid by Thermal Closed System

    Directory of Open Access Journals (Sweden)

    Kuang Meng

    2016-01-01

    Full Text Available In this research, a purification approach using nitric acid leaching at thermal closed system was developed to improve the porous structure of raw diatomite by removal of impurities from its surface and clogged pores. The feasibility and efficiency of this approach were determined by XRF for chemical constitution of diatomite, SEM for morphology and BET for specific surface area of purified diatomite. The investigations indicated that the content of SiO2 was in order of 85.14% for raw diatomite and 98% for purified diatomite, the content of Fe2O3 decreases after purified; the integrity of the porous structure was confirmed by SEM, and increase in specific surface area from 18m2·g-1 to 36m2·g-1.

  13. Processing and removal of the Three Mile Island makeup and purification system resins

    International Nuclear Information System (INIS)

    Reilly, J.K.; McIntosh, T.W.; Northey, L.M.; GaTanto, J.J.; Osterhoudt, T.R.; Thompson, J.D.

    1985-01-01

    The accident at Three Mile Island Unit 2 in March 1979 left the makeup and purification demineralizers inaccessible to workers. These demineralizers contained the highest concentrations of radioactive isotopes outside the Reactor Building. Additionally, unknown quantities of fuel were in the demineralizers. Other concerns centered on the unknown condition of the demineralizer resins, the possible loss of system integrity, and the presence of hydrogen due to the radiolytic breakdown of water contained in the demineralizers. GPU Nuclear, with the US Department of Energy and its contractors EG and G Idaho, Inc., Oak Ridge National Laboratory, and Westinghouse Hanford Company, remotely measured the fuel and fission product inventory of the demineralizers and demonstrated a practical chemical processing method using samples acquired from the demineralizers. Equipment was developed and installed to elute the radioactive cesium from the demineralizers. And elution, which began in September 1984 now nears completion

  14. LHC II system sensitivity to magnetic fluids

    CERN Document Server

    Cotae, Vlad

    2005-01-01

    Experiments have been designed to reveal the influences of ferrofluid treatment and static magnetic field exposure on the photosynthetic system II, where the light harvesting complex (LHC II) controls the ratio chlorophyll a/ chlorophyll b (revealing, indirectly, the photosynthesis rate). Spectrophotometric measurement of chlorophyll content revealed different influences for relatively low ferrofluid concentrations (10-30 mul/l) in comparison to higher concentrations (70-100 mul/l). The overlapped effect of the static magnetic field shaped better the stimulatory ferrofluid action on LHC II system in young poppy plantlets.

  15. Design of functional guanidinium ionic liquid aqueous two-phase systems for the efficient purification of protein

    Energy Technology Data Exchange (ETDEWEB)

    Ding, Xueqin; Wang, Yuzhi, E-mail: wyzss@hnu.edu.cn; Zeng, Qun; Chen, Jing; Huang, Yanhua; Xu, Kaijia

    2014-03-01

    Graphical abstract: - Highlights: • A series of novel cationic functional hexaalkylguanidinium ionic liquids and anionic functional tetraalkylguanidinium ionic liquids have been synthesized. • Functional guanidinium ionic liquid aqueous two-phase systems have been first designed for the purification of protein. • Mechanisms and performances of the process were researched. • Simple, green, safety and presents better purified ability than ordinary process. • A potential efficient platform for protein purification and related studies. - Abstract: A series of novel cationic functional hexaalkylguanidinium ionic liquids and anionic functional tetraalkylguanidinium ionic liquids have been devised and synthesized based on 1,1,3,3-tetramethylguanidine. The structures of the ionic liquids (ILs) were confirmed by {sup 1}H nuclear magnetic resonance ({sup 1}H NMR) and 13C nuclear magnetic resonance (13C NMR) and the production yields were all above 90%. Functional guanidinium ionic liquid aqueous two-phase systems (FGIL-ATPSs) have been first designed with these functional guanidinium ILs and phosphate solution for the purification of protein. After phase separation, proteins had transferred into the IL-rich phase and the concentrations of proteins were determined by measuring the absorbance at 278 nm using an ultra violet visible (UV–vis) spectrophotometer. The advantages of FGIL-ATPSs were compared with ordinary ionic liquid aqueous two-phase systems (IL-ATPSs). The proposed FGIL-ATPS has been applied to purify lysozyme, trypsin, ovalbumin and bovine serum albumin. Single factor experiments were used to research the effects of the process, such as the amount of ionic liquid (IL), the concentration of salt solution, temperature and the amount of protein. The purification efficiency reaches to 97.05%. The secondary structure of protein during the experimental process was observed upon investigation using UV–vis spectrophotometer, Fourier-transform infrared

  16. Alternative disinfection technology for water purification systems; Josui shori ni okeru enso daitai shodoku gijutsu

    Energy Technology Data Exchange (ETDEWEB)

    Aizawa, T. [The Institute of Public Health, Tokyo (Japan)

    1998-09-10

    This paper describes chlorination substituting disinfection technologies used in water purification systems. Chloramine treatment is regarded as effective in reducing trihalomethane (THM). Chlorine is injected in the initial stage in the form of free chlorine to disinfect pathogenic microorganisms in a short time, which is then added with ammonia to convert it into chloramine for further utilization. Chlorine dioxide has not been used in Japan, but introduced in Europe and America to treat THM. Ozone has the strongest oxidizing power, and is used for disinfection, virus inactivation, decomposition of THM precursors, and removal of fungus odor. The ozone treatment will produce aldehyde if an organic matter is present, but aldehyde can be removed by treatment using organismic activated carbon. Ultraviolet ray treatment has an advantage of being difficult of producing byproducts. This system was experimentally compared with free chlorine treatment on disinfection effect, mutagenicity, suppression of producing THM byproducts, and odor removal. In order to assure reliability of microorganismic and chemical safety in tap water supply systems, assurance by considering the entire system is important, not only by operating the disinfection units, but also combining such physical water purifying technologies as coagulation, sedimentation, filtration, and membrane treatment. The use of chlorine substituting disinfectants is also a part of the conception. 6 refs., 8 figs., 5 tabs.

  17. Waste water purification by magnetic separation technique using HTS bulk magnet system

    International Nuclear Information System (INIS)

    Oka, T.; Kanayama, H.; Tanaka, K.; Fukui, S.; Ogawa, J.; Sato, T.; Ooizumi, M.; Terasawa, T.; Itoh, Y.; Yabuno, R.

    2009-01-01

    We have investigated the feasibility of strong magnetic field generators composed of the high temperature superconducting (HTS) bulk magnet systems to the magnetic separation techniques for the waste water including thin emulsion bearing the cutting oil. Two types of the strong field generators were prepared by the face-to-face HTS bulk magnet systems, which emit the magnetic field density of 1 and 2 T in the open spaces between the magnetic poles activated by the pulsed field magnetization and the field cooling methods, respectively. A couple of water channels containing iron balls were settled in the strong field to trap the magnetized flocks in the waste water. The separation ratios of flocks containing 200 ppm magnetite powder were evaluated with respect to the flow rates of the waste water. The performances of bulk magnet system have kept showing values of around 100% until the flowing rate reached up to 18 l/min. This suggests that the magnetic separation by using bulk magnets is effective for the practical water purification systems.

  18. Design of functional guanidinium ionic liquid aqueous two-phase systems for the efficient purification of protein.

    Science.gov (United States)

    Ding, Xueqin; Wang, Yuzhi; Zeng, Qun; Chen, Jing; Huang, Yanhua; Xu, Kaijia

    2014-03-07

    A series of novel cationic functional hexaalkylguanidinium ionic liquids and anionic functional tetraalkylguanidinium ionic liquids have been devised and synthesized based on 1,1,3,3-tetramethylguanidine. The structures of the ionic liquids (ILs) were confirmed by (1)H nuclear magnetic resonance ((1)H NMR) and 13C nuclear magnetic resonance (13C NMR) and the production yields were all above 90%. Functional guanidinium ionic liquid aqueous two-phase systems (FGIL-ATPSs) have been first designed with these functional guanidinium ILs and phosphate solution for the purification of protein. After phase separation, proteins had transferred into the IL-rich phase and the concentrations of proteins were determined by measuring the absorbance at 278 nm using an ultra violet visible (UV-vis) spectrophotometer. The advantages of FGIL-ATPSs were compared with ordinary ionic liquid aqueous two-phase systems (IL-ATPSs). The proposed FGIL-ATPS has been applied to purify lysozyme, trypsin, ovalbumin and bovine serum albumin. Single factor experiments were used to research the effects of the process, such as the amount of ionic liquid (IL), the concentration of salt solution, temperature and the amount of protein. The purification efficiency reaches to 97.05%. The secondary structure of protein during the experimental process was observed upon investigation using UV-vis spectrophotometer, Fourier-transform infrared spectroscopy (FT-IR) and circular dichroism spectrum (CD spectrum). The precision, stability and repeatability of the process were investigated. The mechanisms of purification were researched by dynamic light scattering (DLS), determination of the conductivity and transmission electron microscopy (TEM). It was suggested that aggregation and embrace phenomenon play a significant role in the purification of proteins. All the results show that FGIL-ATPSs have huge potential to offer new possibility in the purification of proteins. Copyright © 2014 Elsevier B.V. All rights

  19. Session II-D. Systems

    International Nuclear Information System (INIS)

    Hall, R.J.

    1981-01-01

    The objectives of the Systems Task in the NWTS Program include: development of program requirements, allocation of the requirements to subsystems or tasks, integration of the task activities towards meeting the overall requirements, and assessment of progress towards achievement of the program mission. The Systems Task also includes a number of ancillary activities which are necessary to the program but which do not logically fall into other work-breakdown structure elements. Activities in the Systems Task, which in the NWTS Program are conducted at both the program and project levels, are generally grouped under the heading systems engineering and include identification of requirements, development of a baseline, integration of the system, baseline control, functional analyses, trade-off studies, and system analyses. The following papers in this session address some of the activities and progress that was achieved in the Systems Task in FY 1981: (1) waste isolation system alternatives: a cost comparison; (2) BWIP technical integration and control; (3) BWIP performance evaluation process: a criteria based method; (4) impacts of waste age; (5) systems studies of subseabed disposal; and (6) systems studies of waste transportation

  20. Purification of Microbially Expressed Recombinant Proteins via a Dual ELP Split Intein System.

    Science.gov (United States)

    Shi, Changhua; Han, Tzu-Chiang; Wood, David W

    2017-01-01

    Fusions of elastin-like peptide (ELP) purification tags and self-cleaving inteins provide a powerful platform for purifying tagless recombinant proteins without the need for conventional packed-bed columns. A drawback to this method has been premature cleaving of the ELP tag during expression, before the purification procedure can take place. Here we demonstrate a split-intein method, where the self-cleaving intein is divided into two inactive segments during expression and purification. Spontaneous assembly of the purified intein segments then restores self-cleaving activity to deliver the tagless target protein.

  1. Characterization of primary coolant purification system samples for assay of spent ion exchanger radionuclide inventor

    International Nuclear Information System (INIS)

    Sajin Prasad, S.; Pant, Amar; Sharma, Ranjit; Pal, Sanjit

    2018-01-01

    The primary coolant system water of a research reactor contains various fission and activation products and the water is circulated continuously through ion exchange resin cartridges, to reduce the radioactive ionic impurity present in it. The coolant purification system comprises of an ion exchange cooler, two micro filters, and a battery of six ion exchanger beds, associated valves, piping and instrumentation (Heavy water System Operating manual, 2014). The spent cartridge is finally disposed off as active solid waste which contains predominantly long lived fission and activation products. The heavy water coolant is also used to cool the structural assemblies after passing through primary heat exchanger and a metallic strainer, which accumulates the fission and activation products. When there is a reduction of coolant flow through these strainers, they are removed for cleaning and decontamination. This paper describes the characterization of ion exchange resin samples and liquid effluent generated during ultra sonic decontamination of strainer. The results obtained can be used as a methodology for the assay of the spent ion exchanger cartridges radionuclide inventory, during its disposal

  2. Thioaptamer Diagnostic System, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — AM Biotechnologies (AM) in partnership with Sandia National Laboratories will develop a Thioaptamer Diagnostic System (TDS) in response to Topic X10.01 Reusable...

  3. Particle Systems and PDEs II

    CERN Document Server

    Soares, Ana

    2015-01-01

    This book focuses on mathematical problems concerning different applications in physics, engineering, chemistry and biology. It covers topics ranging from interacting particle systems to partial differential equations (PDEs), statistical mechanics and dynamical systems. The purpose of the second meeting on Particle Systems and PDEs was to bring together renowned researchers working actively in the respective fields, to discuss their topics of expertise and to present recent scientific results in both areas. Further, the meeting was intended to present the subject of interacting particle systems, its roots in and impacts on the field of physics, and its relation with PDEs to a vast and varied public, including young researchers. The book also includes the notes from two mini-courses presented at the conference, allowing readers who are less familiar with these areas of mathematics to more easily approach them. The contributions will be of interest to mathematicians, theoretical physicists and other researchers...

  4. SOUL System Maturation, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — Busek Co. Inc. proposes to advance the maturity of an innovative Spacecraft on Umbilical Line (SOUL) System suitable for a wide variety of applications of interest...

  5. Analysis of helium purification system capability during water ingress accident in RDE

    Science.gov (United States)

    Sriyono; Kusmastuti, Rahayu; Bakhri, Syaiful; Sunaryo, Geni Rina

    2018-02-01

    The water ingress accident caused by steam generator tube rupture (SGTR) in RDE (Experimental Power Reactor) must be anticipated. During the accident, steam from secondary system diffused and mixed with helium gas in the primary coolant. To avoid graphite corrosion in the core, steam will be removed by Helium purification system (HPS). There are two trains in HPS, first train for normal operation and the second for the regeneration and accident. The second train is responsible to clean the coolant during accident condition. The second train is equipped with additional component, i.e. water cooler, post accident blower, and water separator to remove this mixture gas. During water ingress, the water release from rupture tube is mixed with helium gas. The water cooler acts as a steam condenser, where the steam will be separated by water separator from the helium gas. This paper analyses capability of HPS during water ingress accident. The goal of the research is to determine the time consumed by HPS to remove the total amount of water ingress. The method used is modelling and simulation of the HPS by using ChemCAD software. The BDBA and DBA scenarios will be simulated. In BDBA scenario, up to 110 kg of water is assumed to infiltrate to primary coolant while DBA is up to 35 kg. By using ChemCAD simulation, the second train will purify steam ingress maximum in 0.5 hours. The HPS of RDE has a capability to anticipate the water ingress accident.

  6. The Analysis of the System of special water purification of Beloyarskaya Nuclear Power Plant unit BN-800

    Science.gov (United States)

    Valtseva, A. I.; Bibik, I. S.

    2017-11-01

    This article discusses how the latest system of special water purification KPF-30, designed specifically for the fourth power unit of Beloyarskaya nuclear power plant, which has a number of advantages over other water purification systems as chemical-physical and technical-economic, environmental, and other industrial indicators. The scheme covered in this article systems of special water purification involves the use of a hydrocyclone at the preliminary stage of water treatment, as a worthy alternative to ion-exchange filters, which can significantly reduce the volume of toxic waste. The world community implements the project of closing the nuclear fuel cycle, there is a need to improve the reliability of the equipment for safe processes and development of critical and supercritical parameters in the nuclear industry. Essentially, on operated NPP units, the only factor that can cost-effectively optimize to improve the reliability of equipment is the water chemistry. System KPF30 meets the principles and criteria of ecological safety, demonstrating the justification for reagent less method of water treatment on the main stages, in which no formation of toxic wastes, leading to irreversible consequences of environmental pollution and helps to conserve water.

  7. Positrons in biomolecular systems. II

    International Nuclear Information System (INIS)

    Glass, J.C.; Graf, G.; Costabal, H.; Ewert, D.H.; English, L.

    1982-01-01

    Pickoff-annihilation parameters, as related to the free volume model, are shown to be indicators of structural fluctuations in membranes and membrane bound proteins. Nitrous oxide anesthetic induces lateral rigidity in a membrane, and an anesthetic mechanism is suggested. Conformational changes of (Na + ,K + )ATPase in natural membrane are observed with ATP and Mg-ion binding. New positron applications to active transport and photosynthetic systems are suggested. (Auth.)

  8. Semi-automated 86Y purification using a three-column system

    International Nuclear Information System (INIS)

    Park, Luke S.; Szajek, Lawrence P.; Wong, Karen J.; Plascjak, Paul S.; Garmestani, Kayhan; Googins, Shawn; Eckelman, William C.; Carrasquillo, Jorge A.; Paik, Chang H.

    2004-01-01

    The separation of 86 Y from 86 Sr was optimized by a semi-automated purification system involving the passage of the target sample through three sequential columns. The target material was dissolved in 4 N HNO 3 and loaded onto a Sr-selective (Sr-Spec) column to retain the 86 Sr. The yttrium was eluted with 4 N HNO 3 onto the second Y-selective (RE-Spec) column with quantitative retention. The RE-Spec column was eluted with a stepwise decreasing concentration of HNO 3 to wash out potential metallic impurities to a waste container. The eluate was then pumped onto an Aminex A5 column with 0.1 N HCl and finally with 3 N HCl to collect the radioyttrium in 0.6-0.8 mL with a >80% recovery. This method enabled us to decontaminate Sr by 250,000 times and label 30 μ g of DOTA-Biotin with a >95% yield

  9. The experimental study of a hybrid solar photo-Fenton and photovoltaic system for water purification

    International Nuclear Information System (INIS)

    Jin, Yanchao; Wang, Yiping; Huang, Qunwu; Zhu, Li; Cui, Yong; Cui, Lingyun; Lin, Chunyan

    2017-01-01

    Highlights: • A new solar photo-Fenton and photovoltaic system was performed for the first time. • Acid Red 26, Malachite Green and Reactive Blue 4 were discolored using the system. • The PV panel of the hybrid system could work under lower temperature. • The system achieved self-sufficient energy and could work autonomously. • Solar spectrum could be made full use for power generation and water purification. - Abstract: A new hybrid system that integrated a photovoltaic (PV) panel with a solar photo-Fenton (SPF) reactor was constructed to treat wastewater and generate electricity for the first time. The decolorization and photovoltaic performances of the hybrid system were tested outdoors by discoloring three dyes: Acid Red 26 (AR26), Malachite Green (MG) and Reactive Blue 4 (RB4). Lab scale experiments also had been done to confirm the impact of temperature on the SPF process. The lab scale results show that SPF process was more efficiency for decoloring the different dyes, compared with dark Fenton. The SPF followed a pseudo-first-order reaction and the reaction rate constant was improved about 3.5, 4.5 and 0.61 times for AR26, RB4 and MG respectively as the wastewater temperature increased from 20 to 50 °C. The decolorization difficulty of the three dyes followed this order: MG > AR26 > RB4. The results of the hybrid systems tested outdoors show that 200 mg/L MG had achieved 98.6% color removal after 3 h of treatment at a low catalyst dose (Fe"2"+ = 5 mg/L) under sunlight. For 100 mg/L MG, 99.3% color removal was observed after 70 min. The treatment time required for decolorization of AR26 and RB4 was more shorter. In the present of the water layer, the wastewater temperature was increased and that of the hybrid system was decreased. The average output power of the hybrid system was more than 12 W and sufficient to drive the system during all of the outdoor experiments. Our results suggest that the system could realize decolorization of different

  10. Purification, crystallization, X-ray diffraction analysis and phasing of an engineered single-chain PvuII restriction endonuclease

    International Nuclear Information System (INIS)

    Meramveliotaki, Chrysi; Kotsifaki, Dina; Androulaki, Maria; Hountas, Athanasios; Eliopoulos, Elias; Kokkinidis, Michael

    2007-01-01

    PvuII is the first type II restriction endonuclease to be converted from its wild-type homodimeric form into an enzymatically active single-chain variant. The enzyme was crystallized and phasing was successfully performed by molecular replacement. The restriction endonuclease PvuII from Proteus vulgaris has been converted from its wild-type homodimeric form into the enzymatically active single-chain variant scPvuII by tandemly joining the two subunits through the peptide linker Gly-Ser-Gly-Gly. scPvuII, which is suitable for the development of programmed restriction endonucleases for highly specific DNA cleavage, was purified and crystallized. The crystals diffract to a resolution of 2.35 Å and belong to space group P4 2 , with unit-cell parameters a = b = 101.92, c = 100.28 Å and two molecules per asymmetric unit. Phasing was successfully performed by molecular replacement

  11. The water purification system for the low background counting test facility of the Borexino experiment at Gran Sasso

    International Nuclear Information System (INIS)

    Balata, M.; Cadonati, L.; Laubenstein, M.; Heusser, G.; Giammarchi, M.G.; Scardaoni, R.; Torri, V.; Cecchet, G.; De Bari, A.; Perotti, A.

    1996-01-01

    The Borexino experiment, for the study of solar neutrino physics, requires radiopurity at the level of 5 x 10 -16 g/g 238 U equivalent (or 6 x 10 -9 Bq/kg) on a detector mass of many tons of scintillator. Feasibility studies are performed in a counting test facility now operating at LNGS, which consists of 4 t of liquid scintillator viewed by 100 photomultipliers and shielded by 100 t of water. The accomplishment of this goal requires the shielding liquid, water, to be at the 10 -13 g/g contamination level (1.2 x 10 -6 Bq/kg) or better. This paper describes the water purification system; it consists of a combination of several purification processes to remove particulate, radioactive ions, dissolved gases and other impurities. Residual contaminations are measured by analytical or direct-counting techniques. For radon measurement, particularly challenging at this low activity levels, a low background counting method has been developed. (orig.)

  12. Upgrading of TARN-II vacuum system

    International Nuclear Information System (INIS)

    Chida, K.; Arakaki, Y.; Yoshizawa, M.; Tomizawa, M.; Tanabe, T.; Katayama, I.

    1994-01-01

    Ion pumps and titanium getter pumps have been increased nearly twice in the TARN-II. The pumping speed per unit length is now improved up to 2/3 times that of TARN-I. An average vacuum pressure of 10 -11 Torr order has been achieved at beam time. Performance of the system after the upgrading is reported. (author)

  13. Formal system of communication and understanding. II

    Energy Technology Data Exchange (ETDEWEB)

    Zsuzsanna, M

    1982-01-01

    For pt.I see IBID., no.5, p.252-8 (1982). In this article G. Pask's (1975) formal theory of dialogues and talk is summarized. Part II describes the talk-environment and modelling. The conscious systems and machine-intelligence are mainly dealt with. Finally a couple of cases with Pask's theory implemented are looked at. 7 references.

  14. Proposal of the system for the biogas purification using vitreous and natural zeolite membranes

    International Nuclear Information System (INIS)

    Ortega Viera, Lianys; Fernández SantanI, Elina; Alfonso Martínez, Félix Enrique; Aguiar RoqueI, Yania; Rodríguez Muñoz, Susana

    2017-01-01

    One way to reduce the effects of climate change is to replace fossil fuels with renewable energy such as biogas, but the amount of hydrogen sulphide in it represents an impediment to its use, because of its negative impacts on human health, materials of construction and the environment, being essential its reduction or elimination. For this reason, it was defined as aim to propose an effective method for the purification of biogas using membranes constructed from vitreous waste materials and natural cuban zeolite. This treatment requires four membranes with their supports and eight valves and could be used by small farmers for the purification of 1.4 m 3 of biogas per day, an amount necessary for the cooking of three meals of a family of five people, being the cost of the biogas purification process equal to 0.06%/m 3 . (author)

  15. Reverse osmosis based water treatment and purification systems for nuclear power installations

    International Nuclear Information System (INIS)

    Epimakhov, V.N.; Olejnik, M.S.; Moskvin, L.N.

    2004-01-01

    Experiments on the realization and service of specialized water treatment and purification plants based on the principle of reverse osmosis filtration of water at the NPU benches of the A.P. Aleksandrov Scientific Research Technological Institute (SRTI) are analyzed. Membrane-sorption unit including module of micro-, ultrafiltration, reverse osmosis and ion exchange with productivity to 0.5 m 3 /h is developed and operated at SRTI. It is demonstrated that reverse osmosis purification of manufacturing water significantly improves service conditions of NPU and decreases salinity [ru

  16. Purification, molecular cloning, and enzymatic properties of a family 12 endoglucanase (EG-II) from fomitopsis palustris: role of EG-II in larch holocellulose hydrolysis.

    Science.gov (United States)

    Shimokawa, Tomoko; Shibuya, Hajime; Nojiri, Masanobu; Yoshida, Shigeki; Ishihara, Mitsuro

    2008-09-01

    A family 12 endoglucanase with a molecular mass of 23,926 Da (EG-II) from the brown-rot basidiomycete Fomitopsis palustris was purified and characterized. One of the roles of EG-II in wood degradation is thought to be to loosen the polysaccharide network in cell walls by disentangling hemicelluloses that are associated with cellulose.

  17. The metacompiler system META-II/X

    International Nuclear Information System (INIS)

    Kneis, W.

    1975-03-01

    It is the objective of this work to demonstrate by the properties of the META-II/X system and concrete compiler implementation for IML that a simple and universally applicable symbol processor allows to develop in a very easy manner precompilers for problem oriented languages. The main feature consists in the fact that no auxiliary routines coded manually had to be added for special implementation. The translation of IML is exclusively defined by the compiler description written in the META language. As a whole, META-II/X proves to be a system which is relatively convenient to handle in automating the translation of explicit languages. The decisive point is the choise of an assembler language as target language allowing to transfer to the assembler level references not completely resolved. Implementation includes the possibility of an uncomplicated transfer of the whole system inclusive of the internal compiler representations. (orig.) [de

  18. SCORPION II persistent surveillance system update

    Science.gov (United States)

    Coster, Michael; Chambers, Jon

    2010-04-01

    This paper updates the improvements and benefits demonstrated in the next generation Northrop Grumman SCORPION II family of persistent surveillance and target recognition systems produced by the Xetron Campus in Cincinnati, Ohio. SCORPION II reduces the size, weight, and cost of all SCORPION components in a flexible, field programmable system that is easier to conceal and enables integration of over fifty different Unattended Ground Sensor (UGS) and camera types from a variety of manufacturers, with a modular approach to supporting multiple Line of Sight (LOS) and Beyond Line of Sight (BLOS) communications interfaces. Since 1998 Northrop Grumman has been integrating best in class sensors with its proven universal modular Gateway to provide encrypted data exfiltration to Common Operational Picture (COP) systems and remote sensor command and control. In addition to feeding COP systems, SCORPION and SCORPION II data can be directly processed using a common sensor status graphical user interface (GUI) that allows for viewing and analysis of images and sensor data from up to seven hundred SCORPION system gateways on single or multiple displays. This GUI enables a large amount of sensor data and imagery to be used for actionable intelligence as well as remote sensor command and control by a minimum number of analysts.

  19. The D0 run II trigger system

    International Nuclear Information System (INIS)

    Schwienhorst, Reinhard; Michigan State U.

    2004-01-01

    The D0 detector at the Fermilab Tevatron was upgraded for Run II. This upgrade included improvements to the trigger system in order to be able to handle the increased Tevatron luminosity and higher bunch crossing rates compared to Run I. The D0 Run II trigger is a highly exible system to select events to be written to tape from an initial interaction rate of about 2.5 MHz. This is done in a three-tier pipelined, buffered system. The first tier (level 1) processes fast detector pick-off signals in a hardware/firmware based system to reduce the event rate to about 1. 5kHz. The second tier (level 2) uses information from level 1 and forms simple Physics objects to reduce the rate to about 850 Hz. The third tier (level 3) uses full detector readout and event reconstruction on a filter farm to reduce the rate to 20-30 Hz. The D0 trigger menu contains a wide variety of triggers. While the emphasis is on triggering on generic lepton and jet final states, there are also trigger terms for specific final state signatures. In this document we describe the D0 trigger system as it was implemented and is currently operating in Run II

  20. Bacterial-based systems for expression and purification of recombinant Lassa virus proteins of immunological relevance

    Directory of Open Access Journals (Sweden)

    Cashman Kathleen A

    2008-06-01

    Full Text Available Abstract Background There is a significant requirement for the development and acquisition of reagents that will facilitate effective diagnosis, treatment, and prevention of Lassa fever. In this regard, recombinant Lassa virus (LASV proteins may serve as valuable tools in diverse antiviral applications. Bacterial-based systems were engineered for expression and purification of recombinant LASV nucleoprotein (NP, glycoprotein 1 (GP1, and glycoprotein 2 (GP2. Results Full-length NP and the ectodomains of GP1 and GP2 were generated as maltose-binding protein (MBP fusions in the Rosetta strains of Escherichia coli (E. coli using pMAL-c2x vectors. Average fusion protein yields per liter of culture for MBP-NP, MBP-GP1, and MBP-GP2 were 10 mg, 9 mg, and 9 mg, respectively. Each protein was captured from cell lysates using amylose resin, cleaved with Factor Xa, and purified using size-exclusion chromatography (SEC. Fermentation cultures resulted in average yields per liter of 1.6 mg, 1.5 mg, and 0.7 mg of purified NP, GP1 and GP2, respectively. LASV-specific antibodies in human convalescent sera specifically detected each of the purified recombinant LASV proteins, highlighting their utility in diagnostic applications. In addition, mouse hyperimmune ascitic fluids (MHAF against a panel of Old and New World arenaviruses demonstrated selective cross reactivity with LASV proteins in Western blot and enzyme-linked immunosorbent assay (ELISA. Conclusion These results demonstrate the potential for developing broadly reactive immunological assays that employ all three arenaviral proteins individually and in combination.

  1. Purification of Water by Aquatic Plants

    OpenAIRE

    Morimitsu, Katsuhito; Kawahigashi, Tatsuo

    2013-01-01

    [Abstract] Water quality purification of many water systems including those occurring in rivers depends to a great degree on water quality purification activities of aquatic plants and microbes. This paper presents a discussion of results, based on laboratory experiments, of purification by aquatic plants.

  2. NSLS-II booster timing system

    International Nuclear Information System (INIS)

    Cheblakov, P.; Karnaev, S.; De Long, J.

    2012-01-01

    NSLS-II light source includes the main storage ring with beam lines and injection part consisting of 200 MeV linac, a full-energy 3 GeV booster synchrotron and two transport lines. The booster timing system is a part of NSLS-II timing system which uses hardware from MicroResearch Finland: Event Generator (EVG) and Event Receivers (EVRs). The booster timing is based on the events coming from NSLS-II EVG: 'Pre-Injection', 'Injection', 'Pre-Extraction', 'Extraction'. These events are referenced to the selected RF bucket of the storage ring and correspond to the first RF bucket of the booster. EVRs provide triggers both for the injection and the extraction pulse devices. EVRs also provide the timing of booster cycle operation and generation of events for cycle-to-cycle updates of pulsed and ramping parameters, and synchronization of the booster beam instrumentation devices. This paper describes the final design of the booster timing system. The timing system functional diagrams and block diagram are presented. (authors)

  3. LCLS-II CRYOMODULE TRANSPORT SYSTEM TESTING

    Energy Technology Data Exchange (ETDEWEB)

    Huque, Naeem [Thomas Jefferson National Accelerator Facility (TJNAF), Newport News, VA (United States); Daly, Edward F. [Thomas Jefferson National Accelerator Facility (TJNAF), Newport News, VA (United States); McGee, Michael W. [Fermi National Accelerator Lab. (FNAL), Batavia, IL (United States)

    2018-04-01

    The Cryomodules (CM) for the Linear Coherent Light Source II (LCLS-II) will be shipped to SLAC (Menlo Park, California) from JLab (Newport News, Virginia) and FNAL (Batavia, Illinois). A transportation system has been designed and built to safely transport the CMs over the road. It uses an array of helical isolator springs to attenuate shocks on the CM to below 1.5g in all directions. The system rides on trailers equipped with Air-Ride suspension, which attenuates vibration loads. The prototype LCLS-II CM (pCM) was driven 750 miles to test the transport system; shock loggers recorded the shock attenuation on the pCM and vacuum gauges were used to detect any compromises in beamline vacuum. Alignment measurements were taken before and after the trip to check whether cavity positions had shifted beyond the ± 0.2mm spec. Passband frequencies and cavity gradients were measured at 2K at the Cryomodule Test Facility (CMTF) at JLab to identify any degradation of CM performance after transportation. The transport system was found to have safely carried the CM and is cleared to begin shipments from JLab and FNAL to SLAC.

  4. PEP-II RF feedback system simulation

    Energy Technology Data Exchange (ETDEWEB)

    Tighe, R [Stanford Linear Accelerator Center, Menlo Park, CA (United States)

    1996-08-01

    A model containing the fundamental impedance of the PEP-II cavity along with the longitudinal beam dynamics and RF feedback system components is in use. It is prepared in a format allowing time-domain as well as frequency-domain analysis and full graphics capability. Matlab and Simulink are control system design and analysis programs (widely available) with many built-in tools. The model allows the use of compiled C-code modules for compute intensive portions. We desire to represent as nearly as possible the components of the feedback system including all delays, sample rates and applicable nonlinearities. (author)

  5. Purification and spectroscopic characterization of photosystem II reaction center complexes isolated with or without Triton X-100.

    NARCIS (Netherlands)

    Eijckelhoff, C.; van Roon, H.; Groot, M.L.; van Grondelle, R.; Dekker, J.P.

    1996-01-01

    The pigment composition of the isolated photosystem II reaction center complex in its most stable and pure form currently is a matter of considerable debate. In this contribution, we present a new method based on a combination of gel filtration chromatography and diode array detection to analyze the

  6. Manganese and the II system in photosynthesis

    International Nuclear Information System (INIS)

    Joyard, Jacques

    1971-01-01

    The evolution during greening of some components of system II of photosynthesis has been followed in plastids extracted from Zea mays grown in the dark. Manganese studies were done by means of neutron activation, electron spin resonance (ESR) was also used in some experiments. Oxygen evolution of isolated plastids was followed by polarography (with a membrane electrode). The evolution of manganese/carotenoids ratio can be divided in three parts. During the first hour of greening, the increase shows an input of Mn in the plastids; then, whereas carotenoids content of those plastids presents no changes, Mn is released in the medium; at last, carotenoids synthesis is parallel to Mn fixation in the plastids, the ratio being constant after 24 hours of greening. From various measurements on chloroplastic manganese, it is shown that the development of system II can be divided in two main phases: during the first one (that is during the first day of light) the components are not yet bound together but the relations become more and more strong. Then, during the last period of the development, the organisation of system II is complete and the transformations of the plastids are parallel to the raise of their activity. (author) [fr

  7. Automated Synthesis of 68Ga-DOTA-TOC: Methodological Aspects and Suitable Technical Solutions for a Cationic Purification System.

    Science.gov (United States)

    Uccelli, Licia; Boschi, Alessandra; Cittanti, Corrado; Martini, Petra; Lodi, Luca; Zappaterra, Elisa; Romani, Simona; Zaccaria, Samanta; Cecconi, Davide; Rambaldi, Ilaria; Santi, Ivan; Panareo, Stefano; Giganti, Melchiore; Bartolomei, Mirco

    2018-05-08

    The PET Gallium-68 isotope has the advantage of being produced from a generator, so it is also available in nuclear medicine departments without a cyclotron. The preparation of Ga-68 DOTA-labelled compounds is actually performed by remotely controlled automated systems developed in order to assure production efficiency, reproducibility of the results, guarantee fast reaction time, to facilitate the synthesis and minimize the radiation exposure. Many automatic synthesis systems are available on the radiopharmaceutical market, and each of these requires the realization of some technical adaptations for routine use. We reported the Ga-68 DOTATOC production by automated cassette-based theranostic synthesizer system used in combination with a disposable GMP grade cassette system for cationic purification. The synthesizer is integrated with the 68Ge/68Ga generator systems and it allows to perform elution, eluate purification and radiolabeling in about 38 minutes. We have performed between January 2016 and January 2017 over 100 [68Ga]Ga-DOTA-TOC preparation and of these only three have failed. The average synthesis yield of radiopharmaceutical production was 54.4 ± 2.3 % and the average radiochemical purity was 96.94 ± 0.74 %. The methodology and the technical solutions adopted have allowed to obtain a high quality radiopharmaceutical product as required by the European Pharmacopoeia. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.

  8. Water purification in Borexino

    Energy Technology Data Exchange (ETDEWEB)

    Giammarchi, M. [Infn Milano (Italy); Balata, M.; Ioannucci, L.; Nisi, S. [Laboratori Nazionali del Gran Sasso (Italy); Goretti, A.; Ianni, A. [Princeton University (United States); Miramonti, L. [Dip. di Fisica dell' Università di Milano e Infn (Italy)

    2013-08-08

    Astroparticle Physics and Underground experiments searching for rare nuclear events, need high purity materials to act as detectors or detector shielding. Water has the advantage of being cheap, dense and easily available. Most of all, water can be purified to the goal of obatining a high level of radiopurity. Water Purification can be achieved by means of a combination of processes, including filtration, reverse osmosis, deionization and gas stripping. The Water Purification System for the Borexino experiment, will be described together with its main performances.

  9. Embedded computer systems for control applications in EBR-II

    International Nuclear Information System (INIS)

    Carlson, R.B.; Start, S.E.

    1993-01-01

    The purpose of this paper is to describe the embedded computer systems approach taken at Experimental Breeder Reactor II (EBR-II) for non-safety related systems. The hardware and software structures for typical embedded systems are presented The embedded systems development process is described. Three examples are given which illustrate typical embedded computer applications in EBR-II

  10. Preliminary study on application of Pd composite membrane in helium purification system of high-temperature gas-cooled reactor

    International Nuclear Information System (INIS)

    Cai Jianhua; Yang Xiaoyong; Wang Jie; Yu Suyuan

    2008-01-01

    Helium purification system (HPS) is the main part of the helium auxiliary system of high-temperature gas-cooled reactors (HTGR), also in fusion reactors. Some exploratory work was carried out on the application of Pd composite membrane in the separation of He and H 2 . A typical single stripper permeator with recycle (SSP) system was designed, based on the design parameters of a small scale He purification test system CIGNE in CADARACHE, CEA, France, and finite element analysis method was used to solve the model. The total length of membrane module is fixed to 0.5 m. The results show that the concentration of H 2 is found to reduce from 1 000 μL/L in feed gas to 5 μL/L in the product He (the upper limitation of HPS in HTGR). And the molar ratio of product He to feed gas is 96.18% with the optimized ratio of sweep gas to retentive gas 0. 3970. It's an exponential distribution of H 2 concentration along the membrane module. The results were also compared with the other two popular designs, two stripper in series permeator (TSSP) and continuous membrane column (CMC). (authors)

  11. Purification of bioethanol effluent in an UASB reactor system with simultaneous biogas formation

    DEFF Research Database (Denmark)

    Torry-Smith, Mads Peter; Sommer, Peter; Ahring, Birgitte Kiær

    2003-01-01

    of these compounds were removed from the BEE in the reactor. Implementation of a UASB purification step was found to be a promising approach to detoxify process water from bioethanol production allowing for recirculation of the process water and reduced production costs.......In this study, the prospect of using an Upflow Anaerobic Sludge Blanket (UASB) reactor for detoxification of process water derived from bioethanol production has been investigated. The bioethanol effluent (BEE) originated from wet oxidized wheat straw fermented by Saccharomyces cerevisiae...

  12. Assessment of Integrated Information System (IIS) in organization ...

    African Journals Online (AJOL)

    Assessment of Integrated Information System (IIS) in organization. ... to enable the Information System (IS) managers, as well as top management to understand the ... since organisational and strategic aspects in IIS should also be considered.

  13. Heterologous expression, purification, crystallization and preliminary X-ray analysis of Trichoderma reesei xylanase II and four variants

    International Nuclear Information System (INIS)

    Wan, Qun; Kovalevsky, Andrey; Zhang, Qiu; Hamilton-Brehm, Scott; Upton, Rosalynd; Weiss, Kevin L.; Mustyakimov, Marat; Graham, David; Coates, Leighton; Langan, Paul

    2013-01-01

    The wild-type protein and four active-site mutants of xylanase II from Trichoderma reesei that catalyzes the hydrolysis of glycosidic bonds in xylan have successfully been crystallized. The crystallization of several structures including ligand-free and protein ligand complexes containing the substrate (xylohexaose) or product (xylotriose) are detailed. Xylanase II from Trichoderma reesei catalyzes the hydrolysis of glycosidic bonds in xylan. Crystallographic studies of this commercially important enzyme have been initiated to investigate its reaction mechanism, substrate binding and dependence on basic pH conditions. The wild-type protein was heterologously expressed in an Escherichia coli host using the defined medium and four active-site amino acids were replaced to abolish its activity (E177Q and E86Q) or to change its pH optimum (N44D and N44H). Cation-exchange and size-exclusion chromatography were used to obtain >90% protein purity. The ligand-free proteins and variant complexes containing substrate (xylohexaose) or product (xylotriose) were crystallized in several different space groups and diffracted to high resolutions (from 1.07 to 1.55 Å)

  14. CcMP-II, a new hemorrhagic metalloproteinase from Cerastes cerastes snake venom: purification, biochemical characterization and amino acid sequence analysis.

    Science.gov (United States)

    Boukhalfa-Abib, Hinda; Laraba-Djebari, Fatima

    2015-01-01

    Snake venom metalloproteinases (SVMPs) are the most abundant components in snake venoms. They are important in the induction of systemic alterations and local tissue damage after envenomation. CcMP-II, which is a metalloproteinase purified from Cerastes cerastes snake venom, was obtained by a combination of gel filtration, ion-exchange and affinity chromatographies. It was homogeneous on SDS-PAGE, with a molecular mass estimated to 35kDa and presents a pI of 5.6. CcMP-II has an N-terminal sequence of EDRHINLVSVADHRMXTKY, with high levels of homology with those of the members of class P-II of SVMPs, which comprises metalloproteinase and disintegrin-like domains together. This proteinase displayed a fibrinogenolytic and hemorrhagic activities. The proteolytic and hemorrhagic activities of CcMP-II were inhibited by EDTA and 1,10-phenanthroline. However, these activities were not affected by aprotinine and PMSF, suggesting that CcMP-II is a zinc-dependent hemorrhagic metalloproteinase with an α-fibrinogenase activity. The hemorrhagic metalloproteinase CcMP-II was also able to hydrolyze extracellular matrix components, such as type IV collagen and laminin. These results indicate that CcMP-II is implicated in the local and systemic bleeding, contributing thus in the toxicity of C. cerastes venom. Copyright © 2014 Elsevier Inc. All rights reserved.

  15. Refined hyperentanglement purification of two-photon systems for high-capacity quantum communication with cavity-assisted interaction

    Energy Technology Data Exchange (ETDEWEB)

    Du, Fang-Fang; Li, Tao; Long, Gui-Lu, E-mail: gllong@tsinghua.edu.cn

    2016-12-15

    Hyperentanglement, defined as the entanglement in multiple degrees of freedom (DOFs) of a photonic quantum system, has attracted much attention recently as it can improve the channel capacity of quantum communication largely. Here we present a refined hyperentanglement purification protocol (hyper-EPP) for two-photon systems in mixed hyperentangled states in both the spatial-mode and polarization DOFs, assisted by cavity quantum electrodynamics. By means of the spatial (polarization) quantum state transfer process, the quantum states that are discarded in the previous hyper-EPPs can be preserved. That is, the spatial (polarization) state of a four-photon system with high fidelity can be transformed into another four-photon system with low fidelity, not disturbing its polarization (spatial) state, which makes this hyper-EPP take the advantage of possessing a higher efficiency.

  16. A Hybrid MPC-PID Control System Design for the Continuous Purification and Processing of Active Pharmaceutical Ingredients

    Directory of Open Access Journals (Sweden)

    Maitraye Sen

    2014-05-01

    Full Text Available In this work, a hybrid MPC (model predictive control-PID (proportional-integral-derivative control system has been designed for the continuous purification and processing framework of active pharmaceutical ingredients (APIs. The specific unit operations associated with the purification and processing of API have been developed from first-principles and connected in a continuous framework in the form of a flowsheet model. These integrated unit operations are highly interactive along with the presence of process delays. Therefore, a hybrid MPC-PID is a promising alternative to achieve the desired control loop performance as mandated by the regulatory authorities. The integrated flowsheet model has been simulated in gPROMSTM (Process System Enterprise, London, UK. This flowsheet model has been linearized in order to design the control scheme. The ability to track the set point and reject disturbances has been evaluated. A comparative study between the performance of the hybrid MPC-PID and a PID-only control scheme has been presented. The results show that an enhanced control loop performance can be obtained under the hybrid control scheme and demonstrate that such a scheme has high potential in improving the efficiency of pharmaceutical manufacturing operations.

  17. Biogas recirculation for simultaneous calcium removal and biogas purification within an expanded granular sludge bed system treating leachate.

    Science.gov (United States)

    Luo, Jinghuan; Lu, Xueqin; Liu, Jianyong; Qian, Guangren; Lu, Yongsheng

    2014-12-01

    Biogas, generated from an expanded granular sludge bed (EGSB) reactor treating municipal solid waste (MSW) leachate, was recirculated for calcium removal from the leachate via a carbonation process with simultaneous biogas purification. Batch trials were performed to optimize the solution pH and imported biogas (CO2) for CaCO3 precipitation. With applicable pH of 10-11 obtained, continuous trials achieved final calcium concentrations of 181-375 mg/L (removal efficiencies≈92.8-96.5%) in the leachate and methane contents of 87.1-91.4% (purification efficiencies≈65.4-82.2%) in the biogas. Calcium-balance study indicates that 23-986 mg Ca/d was released from the bio-system under the carbonized condition where CaCO3 precipitating was moved outside the bioreactor, whereas 7918-9517 mg Ca/d was trapped into the system for the controlled one. These findings demonstrate that carbonation removal of calcium by biogas recirculation could be a promising alternative to pretreat calcium-rich MSW leachate and synergistically to improve methane content. Copyright © 2014 Elsevier Ltd. All rights reserved.

  18. Sequential Sedimentation-Biofiltration System for the purification of a small urban river (the Sokolowka, Lodz) supplied by stormwater.

    Science.gov (United States)

    Szklarek, S; Wagner, I; Jurczak, T; Zalewski, M

    2018-01-01

    The study analyses the efficiency of a Sequentional Sedimentation-Biofiltration System (SSBS) built on the Sokolowka river in Lodz (Poland). It was constructed to purify a small urban river whose hydrological regime is dominated by stormwater and meltwater. The SSBS was constructed on a limited area as multi-zone constructed wetlands. The SSBS consists of three zones: sedimentation zone with structures added to improve sedimentation, a geochemical barrier made of limestone deposit and biofiltration zone. The purification processes of total suspended solids (TSS), total phosphorus (TP), total nitrogen (TP) and other nutrients: phosphates (PO 4 3- ), ammonium (NH 4 + ) and nitrates (NO 3 - ) of the SSBS were analyzed. Chloride (Cl - ) reduction was investigated. Monitoring conducted in the first two hydrological years after construction indicated that the SSBS removed 61.4% of TSS, 37.3% of TP, 30.4% of PO 4 3- , 46.1% of TN, 2.8% of NH4+, 44.8% of NO 3 - and 64.0% of Cl - . The sedimentation zone played a key role in removing TSS and nutrients. The geochemical barrier and biofiltration zone each significantly improved overall efficiency by 4-10% for TSS, PO 4 3- , TN, NO 3 - and Cl - . Although the system reduced the concentration of chloride, further studies are needed to determine the circulation of Cl - in constructed wetlands (CWs), and to assess its impact on purification processes. Copyright © 2017 Elsevier Ltd. All rights reserved.

  19. Cloning, expression, and purification of recombinant protein MPT-64 from a virulent strain of Mycobacterium bovis in a prokaryotic system.

    Science.gov (United States)

    Tashakkori, Maryam Mohammadi; Tebianian, Majid; Tabatabaei, Mohammad; Mosavari, Nader

    2016-12-01

    Tuberculosis (TB) is a zoonotic infectious disease common to humans and animals that is caused by the rod-shaped acid-fast bacterium Mycobacterium bovis. Rapid and sensitive detection of TB is promoted by specific antigens. Virulent strains of the TB complex from M. bovis contain 16 regions of difference (RD) in their genome that encode important proteins, including major protein of Mycobacterium Tuberculosis 64 (MBT-64, which is a primary immune-stimulating antigen encoded by RD-2. In this study, we cloned, expressed, and purified MPT-64 as a potent M. bovis antigen in a prokaryotic system for use in future diagnostic studies. The antigenic region of the Mpt64 gene was investigated by bioinformatics methods, cloned into the PQE-30 plasmid, and expressed in Escherichia coli M15 cells, followed by isopropyl β-d-1-thiogalactopyranoside induction. The expressed protein was analyzed sodium dodecyl sulfate polyacrylamide gel electrophoresis and purified using a nickel-affinity column. Biological activity was confirmed by western blot using specific antibodies. Our data verified the successful cloning of the Mpt64 gene (687-bp segment) via the expression vector and purification of recombinant MPT-64 as a 24-kDa protein. These results indicated successful expression and purification of recombinant MPT-64 protein in a prokaryotic system. This protein can be used for serological diagnosis, improved detection of pathogenicity and non-pathogenicity between infected cattle, and for verification of suspected cases of bovine TB. Copyright © 2016.

  20. Extraction and purification of capsaicin from capsicum oleoresin using an aqueous two-phase system combined with chromatography.

    Science.gov (United States)

    Fan, Yong; Lu, Yan-Min; Yu, Bin; Tan, Cong-Ping; Cui, Bo

    2017-09-15

    Capsaicin was extracted from capsicum oleoresin using an aqueous two-phase system (ATPS) composed of an ethylene oxide-propylene oxide (EOPO) copolymer, salt and ethanol. Capsaicin was concentrated in the top polymer-rich phase. To determine the optimal conditions, the partitioning of capsaicin in the ATPS was investigated, considering a single-factor experiment including the salt concentration, polymer concentration, buffer pH, ethanol concentration, sample loading and extraction duration. Response surface methodology was applied to investigate the effects of the polymer concentration, buffer pH and sample loading on capsaicin partitioning. A capsaicin yield of 95.5% was obtained using the optimal extraction system, which consisted of 16.3% UCON 50-HB-5100/10% K 2 HPO 4 /1% ethanol, a buffer pH of 4.35 and 0.24g of capsicum oleoresin. Capsaicin was purified from the capsaicinoid extract using a two-step macroporous adsorption resin (MAR) method. After purification using non-polar MAR ADS-17, the recovery and purity of capsaicin were 83.7% and 50.3%, respectively. After purification using weakly polar MAR AB-8, the recovery and purity of capsaicin were 88.0% and 85.1%, respectively. Copyright © 2017. Published by Elsevier B.V.

  1. Efficient system of artificial oil bodies for functional expression and purification of recombinant nattokinase in Escherichia coli.

    Science.gov (United States)

    Chiang, Chung-Jen; Chen, Hong-Chen; Chao, Yun-Peng; Tzen, Jason T C

    2005-06-15

    Nattokinase, a serine protease, and pronattokinase, when expressed in Escherichia coli, formed insoluble aggregates without enzymatic activity. For functional expression and purification, nattokinase or pronattokinase was first overexpressed in E. coli as an insoluble recombinant protein linked to the C terminus of oleosin, a structural protein of seed oil bodies, by an intein fragment. Artificial oil bodies were reconstituted with triacylglycerol, phospholipid, and the insoluble recombinant protein thus formed. Soluble nattokinase was subsequently released through self-splicing of intein induced by temperature alteration, with the remaining oleosin-intein residing in oil bodies and the leading propeptide of pronattokinase, when present, spontaneously cleaved in the process. Active nattokinase with fibrinolytic activity was harvested by concentrating the supernatant. Nattokinase released from oleosin-intein-pronattokinase exhibited 5 times higher activity than that released from oleosin-intein-nattokinase, although the production yields were similar in both cases. Furthermore, active nattokinase could be harvested in the same system by fusing pronattokinase to the N terminus of oleosin via a different intein linker, with self-splicing induced by 1,4-dithiothreitol. These results have shown a great potential of this system for bacterial expression and purification of functional recombinant proteins.

  2. Impurity impacts on the purification process in oxy-fuel combustion based CO2 capture and storage system

    International Nuclear Information System (INIS)

    Li, H.; Yan, J.; Yan, J.; Anheden, M.

    2009-01-01

    Based on the requirements of CO 2 transportation and storage, non-condensable gases, such as O 2 , N 2 and Ar should be removed from the CO 2 -stream captured from an oxy-fuel combustion process. For a purification process, impurities have great impacts on the design, operation and optimization through their impacts on the thermodynamic properties of CO 2 -streams. Study results show that the increments of impurities will make the energy consumption of purification increase; and make CO 2 purity of separation product and CO 2 recovery rate decrease. In addition, under the same operating conditions, energy consumptions have different sensitivities to the variation of the impurity mole fraction of feed fluids. The isothermal compression work is more sensitive to the variation of SO 2 ; while the isentropic compression work is more sensitive to the variation of Ar. In the flash system, the energy consumption of condensation in is more sensitive to the variation of Ar; but in the distillation system, the energy consumption of condensation is more sensitive to the variation of SO 2 , and CO 2 purity of separation is more sensitive to the variation of SO 2 . (author)

  3. Recovery and purification of ethylene

    Science.gov (United States)

    Reyneke, Rian [Katy, TX; Foral, Michael J [Aurora, IL; Lee, Guang-Chung [Houston, TX; Eng, Wayne W. Y. [League City, TX; Sinclair, Iain [Warrington, GB; Lodgson, Jeffery S [Naperville, IL

    2008-10-21

    A process for the recovery and purification of ethylene and optionally propylene from a stream containing lighter and heavier components that employs an ethylene distributor column and a partially thermally coupled distributed distillation system.

  4. Close loop gas recirculation and purification system for INO RPC system

    International Nuclear Information System (INIS)

    Joshi, A.V.; Kalmani, S.D.; Mondal, N.K.; Satyanarayana, B.; Verma, P.

    2013-01-01

    Close loop gas recirculation system (CLS) is designed to overcome problems. The present system is a pilot unit catering to about 12 RPC detectors of 2m ÃâĂŤ 2m size. The gas mixture is prepared in required concentration, in-situ, and circulated throughout the loop at controlled flow rates. The pressure band is adjusted to be within 20mm of water column. A Programmable Logic Controller (PLC) keeps track of pressure and flow rates, process sequence and safety conditions. The loss of gas is continuously monitored to assess effectiveness of CLS. The concentration of gas components in the mixtures is monitored by sampling through Residual Gas Analyzer (RGA). The RPC performance parameters, such as leakage current, noise rate, efficiency and cross-talk are monitored vis-a-vis CLS parameters. It has been found that RPC parameters respond in coordination with CLS functioning. Room pressure and temperature also seem to have influence on both of them

  5. Theoretical analysis of a biogas-fed PEMFC system with different hydrogen purifications: Conventional and membrane-based water gas shift processes

    International Nuclear Information System (INIS)

    Authayanun, Suthida; Aunsup, Pounyaporn; Patcharavorachot, Yaneeporn; Arpornwichanop, Amornchai

    2014-01-01

    Highlights: • Thermodynamic analysis of the biogas-fed PEMFC system is performed. • Conventional and membrane-based WGS processes for H 2 purification are studied. • A flowsheet model of the PEMFC system is developed. • Effect of key parameters on yields of H 2 and carbon in the biogas reformer is shown. • Performance of PEMFC systems with different H 2 purification processes is analyzed. - Abstract: This study presents a thermodynamic analysis of biogas reforming and proton electrolyte membrane fuel cell (PEMFC) integrated process with different hydrogen purifications: conventional and membrane-based water gas shift processes. The aim is to determine the optimal reforming process for hydrogen production from biogas in the PEMFC system. The formation of carbon is concerned in the hydrogen production. The simulation results show that increases in the steam-to-methane ratio and reformer temperature can improve the hydrogen yield and reduce the carbon formation. From the performance analysis, it is found that when the PEMFC is operated at high temperature and fuel utilization, the overall system efficiency enhances. The performance of the PEMFC system with the installation of a water gas shift membrane unit in the hydrogen purification step is slightly increased, compared with a conventional process

  6. Efficient production and purification of functional bacteriorhodopsin with a wheat-germ cell-free system and a combination of Fos-choline and CHAPS detergents.

    Science.gov (United States)

    Genji, Takahisa; Nozawa, Akira; Tozawa, Yuzuru

    2010-10-01

    Cell-free translation is one potential approach to the production of functional transmembrane proteins. We have now examined various detergents as supplements to a wheat-germ cell-free system in order to optimize the production and subsequent purification of a functional model transmembrane protein, bacteriorhodopsin. We found that Fos-choline and CHAPS detergents counteracted each other's inhibitory effects on cell-free translation activity and thereby allowed the efficient production and subsequent purification of functional bacteriorhodopsin in high yield. Copyright © 2010 Elsevier Inc. All rights reserved.

  7. The CDF-II silicon tracking system

    Energy Technology Data Exchange (ETDEWEB)

    F. Palmonari et al.

    2002-01-18

    The CDFII silicon tracking system, SVX, for Run II of the Fermilab Tevatron has up to 8 cylindrical layers with average radii spanning from {approx} (1.5 to 28.7) cm, and lengths ranging from {approx} (90 to 200) cm for a total active-area of {approx} 6 m{sup 2} and {approx} 7.2 x 10{sup 5} readout channels. SVX will improve the CDFII acceptance and efficiency for both B and high-Pt physics dependent upon b-tagging. Along with the description of the SVX we report some alignment survey data from the SVX assembly phase and the actual status of the alignment as it results from the offline data analysis. The problems encountered are also reviewed.

  8. Direct purification of pectinase from mango (Mangifera Indica Cv. Chokanan) peel using a PEG/salt-based Aqueous Two Phase System.

    Science.gov (United States)

    Mehrnoush, Amid; Sarker, Md Zaidul Islam; Mustafa, Shuhaimi; Yazid, Abdul Manap Mohd

    2011-10-10

    An Aqueous Two-Phase System (ATPS) was employed for the first time for the separation and purification of pectinase from mango (Mangifera Indica Cv. Chokanan) peel. The effects of different parameters such as molecular weight of the polymer (polyethylene glycol, 2,000-10,000), potassium phosphate composition (12-20%, w/w), system pH (6-9), and addition of different concentrations of neutral salts (0-8%, w/w) on partition behavior of pectinase were investigated. The partition coefficient of the enzyme was decreased by increasing the PEG molecular weight. Additionally, the phase composition showed a significant effect on purification factor and yield of the enzyme. Optimum conditions for purification of pectinase from mango peel were achieved in a 14% PEG 4000-14% potassium phosphate system using 3% (w/w) NaCl addition at pH 7.0. Based on this system, the purification factor of pectinase was increased to 13.2 with a high yield of (97.6%). Thus, this study proves that ATPS can be an inexpensive and effective method for partitioning of pectinase from mango peel.

  9. Purification of a fibrinolytic protease from Mucor subtilissimus UCP 1262 by aqueous two-phase systems (PEG/sulfate).

    Science.gov (United States)

    Nascimento, Thiago Pajeú; Sales, Amanda Emmanuelle; Porto, Camila Souza; Brandão, Romero Marcos Pedrosa; de Campos-Takaki, Galba Maria; Teixeira, José Antônio Couto; Porto, Tatiana Souza; Porto, Ana Lúcia Figueiredo; Converti, Attilio

    2016-07-01

    A fibrinolytic protease from M. subtilissimus UCP 1262 was recovered and partially purified by polyethylene glycol (PEG)/sodium sulfate aqueous two-phase systems (ATPS). The simultaneous influence of PEG molar mass, PEG concentration and sulfate concentration on the enzyme recovery was first investigated using a 2(3) full factorial design, and the Response Surface Methodology used to identify the optimum conditions for enzyme extraction by ATPS. Once the best PEG molar mass for the process had been selected (6000g/mol), a two-factor central composite rotary design was applied to better evaluate the effects of the other two independent variables. The fibrinolytic enzyme was shown to preferentially partition to the bottom phase with a partition coefficient (K) ranging from 0.2 to 0.7. The best results in terms of enzyme purification were obtained with the system formed by 30.0% (w/w) PEG 6000g/mol and 13.2% (w/w) sodium sulfate, which ensured a purification factor of 10.0, K of 0.2 and activity yield of 102.0%. SDS-PAGE and fibrin zymography showed that the purified protease has a molecular mass of 97kDa and an apparent isoelectric point of 5.4. When submitted to assays with different substrates and inhibitors, it showed selectivity for succinyl-l-ala-ala-pro-l-phenylalanine-p-nitroanilide and was almost completely inhibited by phenylmethylsulfonyl fluoride, behaving as a chymotrypsin-like protease. At the optimum temperature of 37°C, the enzyme residual activity was 94 and 68% of the initial one after 120 and 150min of incubation, respectively. This study demonstrated that M. subtilissimus protease has potent fibrinolytic activity compared with similar enzymes produced by solid-state fermentation, therefore it may be used as an agent for the prevention and therapy of thrombosis. Furthermore, it appears to have the advantages of low cost production and simple purification. Copyright © 2016 Elsevier B.V. All rights reserved.

  10. A gas circulation and purification system for gas-cell-based low-energy RI-beam production

    Energy Technology Data Exchange (ETDEWEB)

    Sonoda, T.; Wada, M.; Katayama, I.; Kojima, T. M.; Reponen, M. [RIKEN Nishina Center for Accelerator-Based Science, 2-1 Hirosawa, Wako, Saitama 351-0198 (Japan); Tsubota, T. [Tokyo KOATSU Co., Ltd., 1-9-8 Shibuya, Shibuyaku, Tokyo 150-0002 (Japan)

    2016-06-15

    A gas circulation and purification system was developed at the RIKEN Radioactive Isotope Beam Factory that can be used for gas-cell-based low-energy RI-beam production. A high-flow-rate gas cell filled with one atmosphere of buffer gas (argon or helium) is used for the deceleration and thermalization of high-energy RI-beams. The exhausted buffer gas is efficiently collected using a compact dry pump and returned to the gas cell with a recovery efficiency of >97%. The buffer gas is efficiently purified using two gas purifiers as well as collision cleaning, which eliminates impurities in the gas. An impurity level of one part per billion is achieved with this method.

  11. The PEP-II abort kicker system

    International Nuclear Information System (INIS)

    Lamare, J de; Donaldson, A.; Kulikov, A. Lipari, J.

    1997-07-01

    The PEP-II project has two storage rings. The HER (High Energy Ring) has up to 1.48 A of electron beam at 9 GeV, and the LER (Low Energy Ring) has up to 2.14 A of positron beam at 3.1 GeV. To protect the HER and LER beam lines in the event of a ring component failure, each ring has an abort kicker system which directs the beam into a dump when a failure is detected. Due to the high current of the beams, the beam kick is tapered from 100% to 80% in 7.33 uS (the beam transit time around the time). This taper distributes the energy evenly across the window which separates the ring from the beam dump such that the window is not damaged. The abort kicker trigger is synchronized with the ion clearing gap of the beam allowing for the kicker field to rise from 0-80% in 370 nS. This report discusses the design of the system controls, interlocks, power supplies, and modulator

  12. The Belle II SVD data readout system

    Energy Technology Data Exchange (ETDEWEB)

    Thalmeier, R., E-mail: Richard.Thalmeier@oeaw.ac.at [Institute of High Energy Physics, Austrian Academy of Sciences, 1050 Vienna (Austria); Adamczyk, K. [H. Niewodniczanski Institute of Nuclear Physics, Krakow 31-342 (Poland); Aihara, H. [Department of Physics, University of Tokyo, Tokyo 113-0033 (Japan); Angelini, C. [Dipartimento di Fisica, Universita’ di Pisa, I-56127 Pisa (Italy); INFN Sezione di Pisa, I-56127 Pisa (Italy); Aziz, T.; Babu, V. [Tata Institute of Fundamental Research, Mumbai 400005 (India); Bacher, S. [H. Niewodniczanski Institute of Nuclear Physics, Krakow 31-342 (Poland); Bahinipati, S. [Indian Institute of Technology Bhubaneswar, Satya Nagar (India); Barberio, E.; Baroncelli, Ti.; Baroncelli, To. [School of Physics, University of Melbourne, Melbourne, Victoria 3010 (Australia); Basith, A.K. [Indian Institute of Technology Madras, Chennai 600036 (India); Batignani, G. [Dipartimento di Fisica, Universita’ di Pisa, I-56127 Pisa (Italy); INFN Sezione di Pisa, I-56127 Pisa (Italy); Bauer, A. [Institute of High Energy Physics, Austrian Academy of Sciences, 1050 Vienna (Austria); Behera, P.K. [Indian Institute of Technology Madras, Chennai 600036 (India); Bergauer, T. [Institute of High Energy Physics, Austrian Academy of Sciences, 1050 Vienna (Austria); Bettarini, S. [Dipartimento di Fisica, Universita’ di Pisa, I-56127 Pisa (Italy); INFN Sezione di Pisa, I-56127 Pisa (Italy); Bhuyan, B. [Indian Institute of Technolog y Guwahati, Assam 781039 (India); Bilka, T. [Faculty of Mathematics and Physics, Charles University, 12116 Prague (Czech Republic); Bosi, F. [INFN Sezione di Pisa, I-56127 Pisa (Italy); and others

    2017-02-11

    The Belle II Experiment at the High Energy Accelerator Research Organization (KEK) in Tsukuba, Japan, will explore the asymmetry between matter and antimatter and search for new physics beyond the standard model. 172 double-sided silicon strip detectors are arranged cylindrically in four layers around the collision point to be part of a system which measures the tracks of the collision products of electrons and positrons. A total of 1748 radiation-hard APV25 chips read out 128 silicon strips each and send the analog signals by time-division multiplexing out of the radiation zone to 48 Flash Analog Digital Converter Modules (FADC). Each of them applies processing to the data; for example, it uses a digital finite impulse response filter to compensate line signal distortions, and it extracts the peak timing and amplitude from a set of several data points for each hit, using a neural network. We present an overview of the SVD data readout system, along with front-end electronics, cabling, power supplies and data processing.

  13. Optimization of serine protease purification from mango (Mangifera indica cv. Chokanan) peel in polyethylene glycol/dextran aqueous two phase system.

    Science.gov (United States)

    Mehrnoush, Amid; Mustafa, Shuhaimi; Sarker, Md Zaidul Islam; Yazid, Abdul Manap Mohd

    2012-01-01

    Mango peel is a good source of protease but remains an industrial waste. This study focuses on the optimization of polyethylene glycol (PEG)/dextran-based aqueous two-phase system (ATPS) to purify serine protease from mango peel. The activity of serine protease in different phase systems was studied and then the possible relationship between the purification variables, namely polyethylene glycol molecular weight (PEG, 4000-12,000 g·mol(-1)), tie line length (-3.42-35.27%), NaCl (-2.5-11.5%) and pH (4.5-10.5) on the enzymatic properties of purified enzyme was investigated. The most significant effect of PEG was on the efficiency of serine protease purification. Also, there was a significant increase in the partition coefficient with the addition of 4.5% of NaCl to the system. This could be due to the high hydrophobicity of serine protease compared to protein contaminates. The optimum conditions to achieve high partition coefficient (84.2) purification factor (14.37) and yield (97.3%) of serine protease were obtained in the presence of 8000 g·mol(-1) of PEG, 17.2% of tie line length and 4.5% of NaCl at pH 7.5. The enzymatic properties of purified serine protease using PEG/dextran ATPS showed that the enzyme could be purified at a high purification factor and yield with easy scale-up and fast processing.

  14. Purification of pectinase from mango (Mangifera indica L. cv. Chokanan) waste using an aqueous organic phase system: a potential low cost source of the enzyme.

    Science.gov (United States)

    Amid, Mehrnoush; Abdul Manap, Mohd Yazid; Mustafa, Shuhaimi

    2013-07-15

    As a novel method of purification, an aqueous organic phase system (AOPS) was employed to purify pectinase from mango waste. The effect of different parameters, such as the alcohol concentration (ethanol, 1-propanol, and 2-propanol), the salt type and concentration (ammonium sulfate, potassium phosphate and sodium citrate), the feed stock crude load, the aqueous phase pH and NaCl concentration, were investigated in the recovery of pectinase from mango peel. The partition coefficient (K), selectivity (S), purification factor (PF) and yield (Y, %) were investigated in this study as important parameters for the evaluation of enzyme recovery. The desirable partition efficiency for pectinase purification was achieved in an AOPS of 19% (w/w) ethanol and 22% (w/w) potassium phosphate in the presence of 5% (w/w) NaCl at pH 7.0. Based on the system, the purification factor of pectinase was enhanced 11.7, with a high yield of 97.1%. Copyright © 2013 Elsevier B.V. All rights reserved.

  15. Diagnosing the PEP-II Injection System

    Energy Technology Data Exchange (ETDEWEB)

    Decker, F.-J.; Donald, M.H.; Iverson, R.H.; Kulikov, A.; Pappas, G.C.; Weaver, M.; /SLAC

    2005-05-09

    The injection of beam into the PEP-II B-Factory, especially into the High Energy Ring (HER) has some challenges. A high background level in the BaBar detector has for a while inhibited us from trickling charge into the HER similar to the Low Energy Ring (LER). Analyzing the injection system has revealed many issues which could be improved. The injection bump between two kickers was not closed, mainly because the phase advance wasn't exactly 180{sup o} and the two kicker strengths were not balanced. Additionally we found reflections which kick the stored beam after the main kick and cause the average luminosity to drop about 3% for a 10 Hz injection rate. The strength of the overall kick is nearly twice as high as the design, indicating a much bigger effective septum thickness. Compared with single beam the background is worse when the HER beam is colliding with the LER beam. This hints that the beam-beam force and the observed vertical blow-up in the HER pushes the beam and especially the injected beam further out to the edge of the dynamic aperture or beyond.

  16. Diagnosing the PEP-II Injection System

    International Nuclear Information System (INIS)

    Decker, F.-J.; Donald, M.H.; Iverson, R.H.; Kulikov, A.; Pappas, G.C.; Weaver, M.; SLAC

    2005-01-01

    The injection of beam into the PEP-II B-Factory, especially into the High Energy Ring (HER) has some challenges. A high background level in the BaBar detector has for a while inhibited us from trickling charge into the HER similar to the Low Energy Ring (LER). Analyzing the injection system has revealed many issues which could be improved. The injection bump between two kickers was not closed, mainly because the phase advance wasn't exactly 180 o and the two kicker strengths were not balanced. Additionally we found reflections which kick the stored beam after the main kick and cause the average luminosity to drop about 3% for a 10 Hz injection rate. The strength of the overall kick is nearly twice as high as the design, indicating a much bigger effective septum thickness. Compared with single beam the background is worse when the HER beam is colliding with the LER beam. This hints that the beam-beam force and the observed vertical blow-up in the HER pushes the beam and especially the injected beam further out to the edge of the dynamic aperture or beyond

  17. Uranium hexafluoride purification

    International Nuclear Information System (INIS)

    Araujo, Eneas F. de

    1986-01-01

    Uranium hexafluoride might contain a large amount of impurities after manufacturing or handling. Three usual methods of purification of uranium hexafluoride were presented: selective sorption, sublimation, and distillation. Since uranium hexafluoride usually is contaminated with hydrogen fluoride, a theoretical study of the phase equilibrium properties was performed for the binary system UF 6 -HF. A large deviation from the ideal solution behaviour was observed. A purification unity based on a constant reflux batch distillation process was developed. A procedure was established in order to design the re boiler, condenser and packed columns for the UF 6 -HF mixture separation. A bench scale facility for fractional distillation of uranium hexafluoride was described. Basic operations for that facility and results extracted from several batches were discussed. (author)

  18. Ionic Liquid-Based Ultrasonic-Assisted Extraction of Secoisolariciresinol Diglucoside from Flaxseed (Linum usitatissimum L. with Further Purification by an Aqueous Two-Phase System

    Directory of Open Access Journals (Sweden)

    Zhi-Jian Tan

    2015-09-01

    Full Text Available In this work, a two-step extraction methodology of ionic liquid-based ultrasonic-assisted extraction (IL-UAE and ionic liquid-based aqueous two-phase system (IL-ATPS was developed for the extraction and purification of secoisolariciresinol diglucoside (SDG from flaxseed. In the IL-UAE step, several kinds of ILs were investigated as the extractants, to identify the IL that affords the optimum extraction yield. The extraction conditions such as IL concentration, ultrasonic irradiation time, and liquid–solid ratio were optimized using response surface methodology (RSM. In the IL-ATPS step, ATPS formed by adding kosmotropic salts to the IL extract was used for further separation and purification of SDG. The most influential parameters (type and concentration of salt, temperature, and pH were investigated to obtain the optimum extraction efficiency. The maximum extraction efficiency was 93.35% under the optimal conditions of 45.86% (w/w IL and 8.27% (w/w Na2SO4 at 22 °C and pH 11.0. Thus, the combination of IL-UAE and IL-ATPS makes up a simple and effective methodology for the extraction and purification of SDG. This process is also expected to be highly useful for the extraction and purification of bioactive compounds from other important medicinal plants.

  19. The PEP II injection kicker system

    International Nuclear Information System (INIS)

    Pappas, G.C.; Donaldson, A.R.; Williams, D.

    1997-07-01

    PEP II or the B Factory consists of two asymmetric storage rings. The injection energy for electrons is 9 GeV, while that for positrons is 3.1 GeV. The bend angle into the high energy ring (HER) is 0.35 m-rad, and the angle into the low energy ring (LER) is 0.575 m-rad. The magnetic length for the HER kicker is 0.85 m, and 0.55 m for the LER kicker. The field produced by the magnet is therefore 123.5 G for the HER, and 132 G for the LER. Each ring has a kicker magnet upstream of the injection line which is used to distort the orbit of the stored beam. An identical magnet downstream of the injection line is used to restore the orbit of the stored beam and inject the incoming beam. The two magnets are driven in parallel by the modulator. The apeture of the magnets is 3.86x3.46 cm (HxV). Therefore the current required to drive the HER is 863 A, while for the LER it is 756 A. The inductance of the magnet is approximately 1.4 uH/m. The current pulse is a critically damped sinusoid with a rise time of less than 300 ns. A kicker system has been designed which can be used for injection of both beams by varying the charge of voltage. The modulator uses a conjugate circuit to match the impedance of the magnet, and coupling to the beam chamber

  20. Facility information system `SOINS-IIS`; Shisetsu joho kanri system `SOINS-IIS`

    Energy Technology Data Exchange (ETDEWEB)

    Shimaoka, S.; Watanabe, M.; Mizuno, Y. [Fuji Electric Co. Ltd., Tokyo (Japan)

    1998-07-10

    With the informatization in the industry, office space is becoming the center of business activities. Also as to the facility control, a facility control system is required which is added with functions of information service to users and the management support system, in addition to the conventional system used mainly for equipment maintenance. Fuji Electric Co. developed a facility information control system, SOINS-IIS (social information system-infrastructure information system), into which the above-mentioned functions were integrated. The features of the system were presented in examples of the introduction to Ichibankan, YRP (Yokosuka Research Park) Center and R and D Center, NTT DoCoMo. The system roughly has an information service function for facility users, function of management for office staff such as tenant management and bill management, management support function for facility owners and planning departments. Beside the above-mentioned functions, in case of YRP Center, for example, the system has functions of management of reservation of meeting rooms, etc., terminal display of common use information and terminal display of information, and many other management support functions. 10 figs.

  1. A Novel Recombinant DNA System for High Efficiency Affinity Purification of Proteins in Saccharomyces cerevisiae

    Directory of Open Access Journals (Sweden)

    Brian H. Carrick

    2016-03-01

    Full Text Available Isolation of endogenous proteins from Saccharomyces cerevisiae has been facilitated by inserting encoding polypeptide affinity tags at the C-termini of chromosomal open reading frames (ORFs using homologous recombination of DNA fragments. Tagged protein isolation is limited by a number of factors, including high cost of affinity resins for bulk isolation and low concentration of ligands on the resin surface, leading to low isolation efficiencies and trapping of contaminants. To address this, we have created a recombinant “CelTag” DNA construct from which PCR fragments can be created to easily tag C-termini of S. cerevisiae ORFs using selection for a nat1 marker. The tag has a C-terminal cellulose binding module to be used in the first affinity step. Microgranular cellulose is very inexpensive and has an effectively continuous ligand on its surface, allowing rapid, highly efficient purification with minimal background. Cellulose-bound proteins are released by specific cleavage of an included site for TEV protease, giving nearly pure product. The tag can be lifted from the recombinant DNA construct either with or without a 13x myc epitope tag between the target ORF and the TEV protease site. Binding of CelTag protein fusions to cellulose is stable to high salt, nonionic detergents, and 1 M urea, allowing stringent washing conditions to remove loosely associated components, as needed, before specific elution. It is anticipated that this reagent could allow isolation of protein complexes from large quantities of yeast extract, including soluble, membrane-bound, or nucleic acid-associated assemblies.

  2. Integration of a photocatalytic multi-tube reactor for indoor air purification in HVAC systems: a feasibility study.

    Science.gov (United States)

    van Walsem, Jeroen; Roegiers, Jelle; Modde, Bart; Lenaerts, Silvia; Denys, Siegfried

    2018-04-24

    This work is focused on an in-depth experimental characterization of multi-tube reactors for indoor air purification integrated in ventilation systems. Glass tubes were selected as an excellent photocatalyst substrate to meet the challenging requirements of the operating conditions in a ventilation system in which high flow rates are typical. Glass tubes show a low-pressure drop which reduces the energy demand of the ventilator, and additionally, they provide a large exposed surface area to allow interaction between indoor air contaminants and the photocatalyst. Furthermore, the performance of a range of P25-loaded sol-gel coatings was investigated, based on their adhesion properties and photocatalytic activities. Moreover, the UV light transmission and photocatalytic reactor performance under various operating conditions were studied. These results provide vital insights for the further development and scaling up of multi-tube reactors in ventilation systems which can provide a better comfort, improved air quality in indoor environments, and reduced human exposure to harmful pollutants.

  3. Space-Ready Advanced Imaging System, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — In this Phase II effort Toyon will increase the state-of-the-art for video/image systems. This will include digital image compression algorithms as well as system...

  4. Component configuration control system development at EBR-II

    International Nuclear Information System (INIS)

    Monson, L.R.; Stratton, R.C.

    1984-01-01

    One ofthe major programs being pursued by the EBR-II Division of Argonne National Laboratory is to improve the reliability of plant control and protection systems. This effort involves looking closely at the present state of the art and needs associated with plant diagnostic, control and protection systems. One of the areas of development at EBR-II involves a component configuration control system (CCCS). This system is a computerized control and planning aid for the nuclear power operator

  5. The various sodium purification techniques

    International Nuclear Information System (INIS)

    Courouau, J.L.; Masse, F.; Rodriguez, G.; Latge, C.; Redon, B.

    1997-01-01

    In the framework of sodium waste treatment, the sodium purification phase plays an essential role in the chain of operations leading to the transformation of the active sodium, considered as waste, into a stable sodium salt. The objectives of the purification operations are: To keep a low impurity level, particularly a low concentration in oxygen and hydrogen, in order to allow its transfer to a processing plant, and in order to avoid risks of plugging and/or corrosion in sodium facilities; To reduce the sodium activity in order to limit the dose rate close to the facilities, and in order to reduce the activity of the liquid and gaseous effluents. After a recall of the different kind of impurities that can be present in sodium, and of the different purification methods that could be associated with, the following points are highlighted: (i) Oxygen and hydrogen purification needs, and presentation of some selection criteria for a purification unit adapted to a sodium processing plant, as well as 2 cold trap concepts that are in accordance with these criteria: PSICHOS and PIRAMIDE. (ii) Tritium reduction in a bulk of liquid sodium by swamping, isotopic exchange, or permeation throughout a membrane. (iii) Caesium trapping on carbonaceous matrix. The main matrices used at present are R.V.C. (Reticulated Vitreous Carbon) and Actitex/Pica products. Tests in the laboratory and on an experimental device have demonstrated the performances of these materials, which are able to reduce sodium activity in Cs 134 and Cs 137 to very low values. The sodium purification processes as regards to the hydrogen, oxygen and caesium, that are aimed at facilitating the subsequent treatment of sodium, are therefore mastered operations. Regarding the operations associated with the reduction of the tritium activity, the methods are in the process of being qualified, or to be qualified. (author)

  6. Gas purification project

    International Nuclear Information System (INIS)

    Broothaerts, J.; Claes, J.; Collard, G.; Goossens, W.; Harnie, R.; Heylen, P.; Vaesen, J.; Beukelaer, R. de; Dubois, G.; Glibert, R.; Mestrez, J.; Zahlen, A.

    1975-06-01

    Conceptual and experimental studies on LMFBR reprocessing and reactor off-gas purification systems are summarized. Iodine sorption on zeolites, low-temperature adsorption of noble gases on charcoal and catalytic oxidation of hydrogen, simulating tritium, are being studied in laboratory set-ups. A pilot loop with 25 m 3 h -1 throughput has been constructed. Results are quoted from the first phase of the iodine removal programme by scrubbing systems. Further extension of the test loop, comprising off-gases conditioning to removal of krypton in a cryodistillation unit, has been prepared. Delay-bed studies on 133 Xe extraction from LWR off-gases are reported. (author)

  7. Present status of the TJ-II remote participation system

    International Nuclear Information System (INIS)

    Vega, J.; Sanchez, E.; Lopez, A.; Portas, A.; Ochando, M.; Ascasibar, E.; Mollinedo, A.; Munoz, J.; Sanchez, A.; Ruiz, M.; Barrera, E.; Lopez, S.; Castro, R.; Lopez, D.

    2005-01-01

    The TJ-II remote participation system (RPS) was designed to extend to Internet the working capabilities provided in the TJ-II local environment, i.e., tracking the TJ-II operation, monitoring/programming data acquisition and control systems, and accessing databases. The TJ-II RPS was based on web and Java technologies because of their open character, security properties and technological maturity. A web server acts as a communication front-end between remote participants and local TJ-II elements. From the server side, web services are provided by means of resources supplied by JSP pages. The client part makes use of web browsers and ad hoc Java applications. The operation requires the use of a distributed authentication and authorization system. This development employs the PAPI System. At present, approximately 1000 digitisation channels can be managed from the TJ-II RPS. Furthermore, processing software based on a 4GL language (LabView) can be downloaded to multiprocessor data acquisition systems. Also, 15 diagnostic control systems, databases and the operation logbook are available from the RPS. The system even allows for the physicist in charge of operation to be in a remote location. Four Spanish universities make use of the TJ-II remote participation system capabilities for joint collaborations: these are the Universidad Politecnica de Madrid (UPM), Universidad Nacional de Educacion a Distancia (UNED), Universidad Complutense de Madrid (UCM) and Universidad Politecnica de Cataluna (UPC)

  8. Prototype system tests of the Belle II PXD DAQ system

    Energy Technology Data Exchange (ETDEWEB)

    Fleischer, Soeren; Gessler, Thomas; Kuehn, Wolfgang; Lange, Jens Soeren; Muenchow, David; Spruck, Bjoern [II. Physikalisches Institut, Justus-Liebig-Universitaet Giessen (Germany); Liu, Zhen' An; Xu, Hao; Zhao, Jingzhou [Institute of High Energy Physics, Chinese Academy of Sciences (China); Collaboration: II PXD Collaboration

    2012-07-01

    The data acquisition system for the Belle II DEPFET Pixel Vertex Detector (PXD) is designed to cope with a high input data rate of up to 21.6 GB/s. The main hardware component will be AdvancedTCA-based Compute Nodes (CN) equipped with Xilinx Virtex-5 FX70T FPGAs. The design for the third Compute Node generation was completed recently. The xTCA-compliant system features a carrier board and 4 AMC daughter boards. First test results of a prototype board will be presented, including tests of (a) The high-speed optical links used for data input, (b) The two 2 GB DDR2-chips on the board and (c) Output of data via ethernet, using UDP and TCP/IP with both hardware and software protocol stacks.

  9. Efficient and versatile one-step affinity purification of in vivo biotinylated proteins: Expression, characterization and structure analysis of recombinant human glutamate carboxypeptidase II

    Czech Academy of Sciences Publication Activity Database

    Tykvart, Jan; Šácha, Pavel; Bařinka, Cyril; Knedlík, Tomáš; Starková, Jana; Lubkowski, J.; Konvalinka, Jan

    2012-01-01

    Roč. 82, č. 1 (2012), s. 106-115 ISSN 1046-5928 R&D Projects: GA MŠk 1M0508; GA MŠk LC512 Institutional research plan: CEZ:AV0Z40550506; CEZ:AV0Z50520701 Keywords : affinity purification * biotin acceptor peptide * recombinant protein expression * biotin -protein ligase (BirA) * co-localization * PSMA Subject RIV: CE - Biochemistry Impact factor: 1.429, year: 2012

  10. HPC Colony II: FAST_OS II: Operating Systems and Runtime Systems at Extreme Scale

    Energy Technology Data Exchange (ETDEWEB)

    Moreira, Jose [IBM, Armonk, NY (United States)

    2013-11-13

    HPC Colony II has been a 36-month project focused on providing portable performance for leadership class machines—a task made difficult by the emerging variety of more complex computer architectures. The project attempts to move the burden of portable performance to adaptive system software, thereby allowing domain scientists to concentrate on their field rather than the fine details of a new leadership class machine. To accomplish our goals, we focused on adding intelligence into the system software stack. Our revised components include: new techniques to address OS jitter; new techniques to dynamically address load imbalances; new techniques to map resources according to architectural subtleties and application dynamic behavior; new techniques to dramatically improve the performance of checkpoint-restart; and new techniques to address membership service issues at scale.

  11. Expression, stabilization and purification of membrane proteins via diverse protein synthesis systems and detergents involving cell-free associated with self-assembly peptide surfactants.

    Science.gov (United States)

    Zheng, Xuan; Dong, Shuangshuang; Zheng, Jie; Li, Duanhua; Li, Feng; Luo, Zhongli

    2014-01-01

    G-protein coupled receptors (GPCRs) are involved in regulating most of physiological actions and metabolism in the bodies, which have become most frequently addressed therapeutic targets for various disorders and diseases. Purified GPCR-based drug discoveries have become routine that approaches to structural study, novel biophysical and biochemical function analyses. However, several bottlenecks that GPCR-directed drugs need to conquer the problems including overexpression, solubilization, and purification as well as stabilization. The breakthroughs are to obtain efficient protein yield and stabilize their functional conformation which are both urgently requiring of effective protein synthesis system methods and optimal surfactants. Cell-free protein synthesis system is superior to the high yields and post-translation modifications, and early signs of self-assembly peptide detergents also emerged to superiority in purification of membrane proteins. We herein focus several predominant protein synthesis systems and surfactants involving the novel peptide detergents, and uncover the advantages of cell-free protein synthesis system with self-assembling peptide detergents in purification of functional GPCRs. This review is useful to further study in membrane proteins as well as the new drug exploration. Copyright © 2014 Elsevier Inc. All rights reserved.

  12. Implementing real-time robotic systems using CHIMERA II

    Science.gov (United States)

    Stewart, David B.; Schmitz, Donald E.; Khosla, Pradeep K.

    1990-01-01

    A description is given of the CHIMERA II programming environment and operating system, which was developed for implementing real-time robotic systems. Sensor-based robotic systems contain both general- and special-purpose hardware, and thus the development of applications tends to be a time-consuming task. The CHIMERA II environment is designed to reduce the development time by providing a convenient software interface between the hardware and the user. CHIMERA II supports flexible hardware configurations which are based on one or more VME-backplanes. All communication across multiple processors is transparent to the user through an extensive set of interprocessor communication primitives. CHIMERA II also provides a high-performance real-time kernel which supports both deadline and highest-priority-first scheduling. The flexibility of CHIMERA II allows hierarchical models for robot control, such as NASREM, to be implemented with minimal programming time and effort.

  13. Experimental study of the portable blood purification system for treatment of acute renal failure in dogs under the field condition in a high-altitude environment

    Directory of Open Access Journals (Sweden)

    Hai-bin LI

    2014-01-01

    Full Text Available Objective To reproduce the model of acute renal failure (ARF in beagles for comprehensively evaluating the safety, stability and validity of the continuous blood purification system under the field condition in a high-altitude environment. Methods Adult beagle ARF model was reproduced by bilateral nephrectomy. All ARF dogs underwent continuous veno-venous hemofiltration with a blood purification machine. Vital signs, renal function, liver function and plasma ion-levels before and after the therapy were analyzed through self-controlled study. Performance of the continuous blood purification machine running in a highaltitude environment was evaluated. Results Blood urea nitrogen (BUN and creatinine (Cr of six dogs were assayed 24 hours after nephrectomy, the difference showed statistical significance compared with those before the surgery (P<0.05, implying that six ARF models were reproduced successfully. Six dogs experienced continuous renal replacement therapy (CRRT with external jugular vein double-lumen tube placement (3 dogs or femoral vein catheter placement (3 dogs. Five dogs successfully finished the whole experiment, and one dog died because of falling off of the dialysis tube and blood loss. The continuous blood purification machine was sensitive with rapid release of warning signals when the condition was abnormal. Compared to that before CRRT, there were no statistically significant differences in heart rate, respiratory rate and blood pressure in ARF models 2, 4 hours after CRRT (P>0.05. And there were no statistically significant differences in ALT, AST and T-Bil in ARF models between that before and 2, 4 hours after CRRT (P>0.05. However, 2, 4 hours after CRRT, BUN and Cr levels descended significantly (P<0. 05, so was K+ level (P<0. 05. There were no complications such as hemorrhage or hypotension. There were no statistical differences in volume of replacement fluid or discarded fluid between before and after CRRT

  14. The proteolytic system of pineapple stems revisited: Purification and characterization of multiple catalytically active forms.

    Science.gov (United States)

    Matagne, André; Bolle, Laetitia; El Mahyaoui, Rachida; Baeyens-Volant, Danielle; Azarkan, Mohamed

    2017-06-01

    Crude pineapple proteases extract (aka stem bromelain; EC 3.4.22.4) is an important proteolytic mixture that contains enzymes belonging to the cysteine proteases of the papain family. Numerous studies have been reported aiming at the fractionation and characterization of the many molecular species present in the extract, but more efforts are still required to obtain sufficient quantities of the various purified protease forms for detailed physicochemical, enzymatic and structural characterization. In this work, we describe an efficient strategy towards the purification of at least eight enzymatic forms. Thus, following rapid fractionation on a SP-Sepharose FF column, two sub-populations with proteolytic activity were obtained: the unbound (termed acidic) and bound (termed basic) bromelain fractions. Following reversible modification with monomethoxypolyethylene glycol (mPEG), both fractions were further separated on Q-Sepharose FF and SP-Sepharose FF, respectively. This procedure yielded highly purified molecular species, all titrating ca. 1 mol of thiol group per mole of enzyme, with distinct biochemical properties. N-terminal sequencing allowed identifying at least eight forms with proteolytic activity. The basic fraction contained previously identified species, i.e. basic bromelain forms 1 and 2, ananain forms 1 and 2, and comosain (MEROPS identifier: C01.027). Furthermore, a new proteolytic species, showing similarities with basic bomelain forms 1 and 2, was discovered and termed bromelain form 3. The two remaining species were found in the acidic bromelain fraction and were arbitrarily named acidic bromelain forms 1 and 2. Both, acidic bromelain forms 1, 2 and basic bromelain forms 1, 2 and 3 are glycosylated, while ananain forms 1 and 2, and comosain are not. The eight protease forms display different amidase activities against the various substrates tested, namely small synthetic chromogenic compounds (DL-BAPNA and Boc-Ala-Ala-Gly-pNA), fluorogenic compounds

  15. The Brookside Farm Wetland Ecosystem Treatment (WET System: A Low-Energy Methodology for Sewage Purification, Biomass Production (Yield, Flood Resilience and Biodiversity Enhancement

    Directory of Open Access Journals (Sweden)

    Julian C. Abrahams

    2017-01-01

    Full Text Available Wastewater from domestic developments, farms and agro-industrial processing can be sources of pollution in the environment; current wastewater management methods are usually machine-based, and thus energy consuming. When Permaculture Principles are used in the creation of water purification and harvesting systems, there can be multiple environmental and economic benefits. In the context of energy descent, it may be considered desirable to treat wastewater using minimal energy. The constructed wetland design presented here is a low-entropy system in which wastewater is harvested and transformed into lush and productive wetland, eliminating the requirement for non-renewable energy in water purification, and also maximising benefits: biodiversity, flood resilience and yield. In permaculture design, the high concentrations of nitrogen and phosphorous compounds in sewage are viewed as valuable nutrients, resources to be harvested by a constructed wetland ecosystem and converted into useful yield. Similarly, rainwater runoff is not viewed as a problem which can cause flooding, but as a potential resource to be harvested to provide a yield. This paper presents a case study, with both water quality and productivity data, from Brookside Farm UK, where the use of Permaculture Design Principles has created a combined wastewater management and purification system, accepting all site water.

  16. Air/Water Purification

    Science.gov (United States)

    1992-01-01

    After 18 years of research into air/water pollution at Stennis Space Center, Dr. B. C. Wolverton formed his own company, Wolverton Environmental Services, Inc., to provide technology and consultation in air and water treatment. Common houseplants are used to absorb potentially harmful materials from bathrooms and kitchens. The plants are fertilized, air is purified, and wastewater is converted to clean water. More than 100 U.S. communities have adopted Wolverton's earlier water hyacinth and artificial marsh applications. Catfish farmers are currently evaluating the artificial marsh technology as a purification system.

  17. Water Purification Product

    Science.gov (United States)

    2004-01-01

    Ecomaster, an affiliate of BioServe Space Technologies, this PentaPure technology has been used to purify water for our nation's Space Shuttle missions since 1981. WTC-Ecomaster of Mirneapolis, Minnesota manufactures water purification systems under the brand name PentaPure (TM). BioServe researcher Dr. George Marchin, of Kansas State University, first demonstrated the superiority of this technology and licensed it to WTC. Marchin continues to perform microgravity research in the development of new technologies for the benefit of life on Earth.

  18. CONCEPTUAL DESIGN OF THE NSLS-II INJECTION SYSTEM.

    Energy Technology Data Exchange (ETDEWEB)

    SHAFTAN,T.; ROSE, T.; PINAYEV, I.; HEESE, R.; BENGTSSON, J.; SKARITKA, J.; MENG, W.; OZAKI, S.; MEIER, R.; STELMACH, C.; LITVINENKO, V.; PJEROV, S.; SHARMA, S.; GANETIS, G.; HSEUH, H.C.; JOHNSON, E.D.; TSOUPAS, N.; GUO, W.; BEEBE-WANG, J.; LUCCIO, A.U.; YU, L.H.; RAPARIA, D.; WANG, D.

    2007-06-25

    We present the conceptual design of the NSLS-II injection system [1,2]. The injection system consists of a low-energy linac, booster and transport lines. We review two different injection system configurations; a booster located in the storage ring tunnel and a booster housed in a separate building. We briefly discuss main parameters and layout of the injection system components.

  19. Partition Efficiency of High-Pitch Locular Multilayer Coil for Countercurrent Chromatographic Separation of Proteins Using Small-Scale Cross-Axis Coil Planet Centrifuge and Application to Purification of Various Collagenases with Aqueous-Aqueous Polymer Phase Systems

    Science.gov (United States)

    Shinomiya, Kazufusa; Kobayashi, Hiroko; Inokuchi, Norio; Nakagomi, Kazuya; Ito, Yoichiro

    2010-01-01

    Partition efficiency of the high-pitch locular multilayer coil was evaluated in countercurrent chromatographic (CCC) separation of proteins with an aqueous-aqueous polymer phase system using the small-scale cross-axis coil planet centrifuge (X-axis CPC) fabricated in our laboratory. The separation column was specially made by high-pitch (ca 5 cm) winding of 1.0 mm I.D., 2.0 mm O.D. locular tubing compressed at 2 cm intervals with a total capacity of 29.5 mL. The protein separation was performed using a set of stable proteins including cytochrome C, myoglobin, and lysozyme with the 12.5% (w/w) polyethylene glycol (PEG) 1000 and 12.5% (w/w) dibasic potassium phosphate system (pH 9.2) under 1000 rpm of column revolution. This high-pitch locular tubing yielded substantially increased stationary phase retention than the normal locular tubing for both lower and upper mobile phases. In order to demonstrate the capability of the high-pitch locular tubing, the purification of collagenase from the crude commercial sample was carried out using an aqueous-aqueous polymer phase system. Using the 16.0% (w/w) PEG 1000 – 6.3% (w/w) dibasic potassium phosphate – 6.3% (w/w) monobasic potassium phosphate system (pH 6.6), collagenase I, II, V and X derived from Clostridium hystolyticum were separated from other proteins and colored small molecular weight compounds present in the crude commercial sample, while collagenase N-2 and S-1 from Streptomyces parvulus subsp. citrinus were eluted with impurities at the solvent front with the upper phase. The collagenase from C. hystolyticum retained its enzymatic activity in the purified fractions. The overall results demonstrated that the high-pitch locular multilayer coil is effectively used for the CCC purification of bioactive compounds without loss of their enzymatic activities. PMID:21869859

  20. Partition Efficiency of High-Pitch Locular Multilayer Coil for Countercurrent Chromatographic Separation of Proteins Using Small-Scale Cross-Axis Coil Planet Centrifuge and Application to Purification of Various Collagenases with Aqueous-Aqueous Polymer Phase Systems.

    Science.gov (United States)

    Shinomiya, Kazufusa; Kobayashi, Hiroko; Inokuchi, Norio; Nakagomi, Kazuya; Ito, Yoichiro

    2011-01-01

    Partition efficiency of the high-pitch locular multilayer coil was evaluated in countercurrent chromatographic (CCC) separation of proteins with an aqueous-aqueous polymer phase system using the small-scale cross-axis coil planet centrifuge (X-axis CPC) fabricated in our laboratory. The separation column was specially made by high-pitch (ca 5 cm) winding of 1.0 mm I.D., 2.0 mm O.D. locular tubing compressed at 2 cm intervals with a total capacity of 29.5 mL. The protein separation was performed using a set of stable proteins including cytochrome C, myoglobin, and lysozyme with the 12.5% (w/w) polyethylene glycol (PEG) 1000 and 12.5% (w/w) dibasic potassium phosphate system (pH 9.2) under 1000 rpm of column revolution. This high-pitch locular tubing yielded substantially increased stationary phase retention than the normal locular tubing for both lower and upper mobile phases. In order to demonstrate the capability of the high-pitch locular tubing, the purification of collagenase from the crude commercial sample was carried out using an aqueous-aqueous polymer phase system. Using the 16.0% (w/w) PEG 1000 - 6.3% (w/w) dibasic potassium phosphate - 6.3% (w/w) monobasic potassium phosphate system (pH 6.6), collagenase I, II, V and X derived from Clostridium hystolyticum were separated from other proteins and colored small molecular weight compounds present in the crude commercial sample, while collagenase N-2 and S-1 from Streptomyces parvulus subsp. citrinus were eluted with impurities at the solvent front with the upper phase. The collagenase from C. hystolyticum retained its enzymatic activity in the purified fractions. The overall results demonstrated that the high-pitch locular multilayer coil is effectively used for the CCC purification of bioactive compounds without loss of their enzymatic activities.

  1. System Aero-Accelator for the purification of biodegradable effluents; Sistema aero-accelator para la depuracion de efluentes biodegradables (I)

    Energy Technology Data Exchange (ETDEWEB)

    Bosque Hernandez, J. L. del; Martin Sanchez, J. L. [Universidad de Salamanca (Spain)

    2000-07-01

    The contamination of the waters is one of the factors that contributes to the deterioration of our environment and since it is a very limited one its treatment descontaminant it is one of the politic's main objectives and environmental administration at all the levels, being spread to the total purification of the generated residual effluents. To reach this objective, big technological efforts are required that allow next to the creation of new processes, the adaptation of the processes existent depuratives, increasing the effectiveness of the same ones. One of the techniques of purification of possible recovery is the Compact System of active mires Aero-Accelator. Presently work is designed and it builds a plant pilot with Aero-Accelator geometry to study its behavior in the treatment of effluents of urban type with different loads pollutants. (Author) 16 refs.

  2. A Novel Aqueous Micellar Two-Phase System Composed of Surfactant and Sorbitol for Purification of Pectinase Enzyme from Psidium guajava and Recycling Phase Components

    Science.gov (United States)

    Murshid, Fara Syazana; Manap, Mohd Yazid; Hussin, Muhaini

    2015-01-01

    A novel aqueous two-phase system composed of a surfactant and sorbitol was employed for the first time to purify pectinase from Psidium guajava. The influences of different parameters, including the type and concentration of the surfactant and the concentration and composition of the surfactant/sorbitol ratio, on the partitioning behavior and recovery of pectinase were investigated. Moreover, the effects of system pH and the crude load on purification fold and the yield of purified pectinase were studied. The experimental results indicated that the pectinase was partitioned into surfactant-rich top phase, and the impurities were partitioned into the sorbitol-rich bottom phase with the novel method involving an ATPS composed of 26% (w/w) Triton X-100 and 23% (w/w) sorbitol at 54.2% of the TLL crude load of 20% (w/w) at pH 6.0. The enzyme was successfully recovered by this method with a high purification factor of 15.2 and a yield of 98.3%, whereas the phase components were also recovered and recycled at rates above 96%. This study demonstrated that this novel ATPS method can be used as an efficient and economical alternative to the traditional ATPS for the purification and recovery of the valuable enzyme. PMID:25756051

  3. A novel aqueous micellar two-phase system composed of surfactant and sorbitol for purification of pectinase enzyme from Psidium guajava and recycling phase components.

    Science.gov (United States)

    Amid, Mehrnoush; Murshid, Fara Syazana; Manap, Mohd Yazid; Hussin, Muhaini

    2015-01-01

    A novel aqueous two-phase system composed of a surfactant and sorbitol was employed for the first time to purify pectinase from Psidium guajava. The influences of different parameters, including the type and concentration of the surfactant and the concentration and composition of the surfactant/sorbitol ratio, on the partitioning behavior and recovery of pectinase were investigated. Moreover, the effects of system pH and the crude load on purification fold and the yield of purified pectinase were studied. The experimental results indicated that the pectinase was partitioned into surfactant-rich top phase, and the impurities were partitioned into the sorbitol-rich bottom phase with the novel method involving an ATPS composed of 26% (w/w) Triton X-100 and 23% (w/w) sorbitol at 54.2% of the TLL crude load of 20% (w/w) at pH 6.0. The enzyme was successfully recovered by this method with a high purification factor of 15.2 and a yield of 98.3%, whereas the phase components were also recovered and recycled at rates above 96%. This study demonstrated that this novel ATPS method can be used as an efficient and economical alternative to the traditional ATPS for the purification and recovery of the valuable enzyme.

  4. Alternative pathways for angiotensin II generation in the cardiovascular system

    Directory of Open Access Journals (Sweden)

    C. Becari

    2011-09-01

    Full Text Available The classical renin-angiotensin system (RAS consists of enzymes and peptides that regulate blood pressure and electrolyte and fluid homeostasis. Angiotensin II (Ang II is one of the most important and extensively studied components of the RAS. The beneficial effects of angiotensin converting enzyme (ACE inhibitors in the treatment of hypertension and heart failure, among other diseases, are well known. However, it has been reported that patients chronically treated with effective doses of these inhibitors do not show suppression of Ang II formation, suggesting the involvement of pathways alternative to ACE in the generation of Ang II. Moreover, the finding that the concentration of Ang II is preserved in the kidney, heart and lungs of mice with an ACE deletion indicates the important role of alternative pathways under basal conditions to maintain the levels of Ang II. Our group has characterized the serine protease elastase-2 as an alternative pathway for Ang II generation from Ang I in rats. A role for elastase-2 in the cardiovascular system was suggested by studies performed in heart and conductance and resistance vessels of normotensive and spontaneously hypertensive rats. This mini-review will highlight the pharmacological aspects of the RAS, emphasizing the role of elastase-2, an alternative pathway for Ang II generation.

  5. The Muon system of the run II D0 detector

    Energy Technology Data Exchange (ETDEWEB)

    Abazov, V.M.; Acharya, B.S.; Alexeev, G.D.; Alkhazov, G.; Anosov, V.A.; Baldin, B.; Banerjee, S.; Bardon, O.; Bartlett, J.F.; Baturitsky, M.A.; Beutel, D.; Bezzubov,; Bodyagin, V.; Butler, J.M.; Cease, H.; Chi, E.; Denisov, D.; Denisov, S.P.; Diehl, H.T.; Doulas, S.; Dugad, S.R.; /Beijing, Inst. High Energy Phys. /Charles U. /Prague, Tech.

    2005-03-01

    The authors describe the design, construction and performance of the upgraded D0 muon system for Run II of the Fermilab Tevatron collider. Significant improvements have been made to the major subsystems of the D0 muon detector: trigger scintillation counters, tracking detectors, and electronics. The Run II central muon detector has a new scintillation counter system inside the iron toroid and an improved scintillation counter system outside the iron toroid. In the forward region, new scintillation counter and tracking systems have been installed. Extensive shielding has been added in the forward region. A large fraction of the muon system electronics is also new.

  6. [Isolation and purification of alpha-glycerophosphate oxidase in a polyethylene glycol/(NH4 )2SO4 aqueous two-phase system].

    Science.gov (United States)

    Meng, Yao; Jin, Jiagui; Liu, Shuangfeng; Yang, Min; Zhang, Qinglian; Wan, Li; Tang, Kun

    2014-02-01

    Alpha-glycerophosphate oxidase (alpha-GPO) from Enterococcus casseli flavus was successfully isolated and purified by using polyethylene glycol (PEG)/(NH4)2SO4 aqueous two-phase system (ATPS). The results showed that the chosen PEG/(NH4)2SO4 ATPS could be affected by PEG molecular weight, pH, concentration of PEG and (NH4)2SO4, and inorganic salt as well as additional amount of crude enzyme. After evaluating these influencing factors, the final optimum purification strategy was formed by 16.5% (m/m) PEG2000, 13.2% (m/m) (NH4)2SO4, pH 7.5 and 30% (m/m) additive crude enzyme, respectively. The NaCl was a negative influencing factor which would lead to lower purification fold and activity recovery. These conditions eventually resulted in the activity recovery of 89% (m/m), distribution coefficient of 1.2 and purification fold of 7.0.

  7. PEP-II RF System Operation and Performance

    International Nuclear Information System (INIS)

    McIntosh, P.

    2005-01-01

    The Low Energy Ring (LER) and High Energy Ring (HER) RF systems have operated now on PEP-II since July 1998 and have assisted in breaking all design luminosity records back in June 2001. Luminosity on PEP-II has steadily increased since then as a consequence of larger e+ and e- beam currents being accumulated. This has meant that the RF systems have inevitably been driven harder, not only to achieve these higher stored beam currents, but also to reliably keep the beams circulating whilst at the same time minimizing the number of aborts due to RF system faults. This paper details the current PEP-II RF system configurations for both rings, as well as future upgrade plans spanning the next 3-5 years. Limitations of the current RF system configurations are presented, highlighting improvement projects which will target specific areas within the RF systems to ensure that adequate operating overheads are maintained and reliable operation is assured. The Low Energy Ring (LER) and High Energy Ring (HER) RF systems have operated now on PEP-II since July 1998 and have assisted in breaking all design luminosity records back in June 2001. Luminosity on PEP-II has steadily increased since then as a consequence of larger e+ and e- beam currents being accumulated. This has meant that the RF systems have inevitably been driven harder, not only to achieve these higher stored beam currents, but also to reliably keep the beams circulating whilst at the same time minimizing the number of aborts due to RF system faults. This paper details the current PEP-II RF system configurations for both rings, as well as future upgrade plans spanning the next 3-5 years. Limitations of the current RF system configurations are presented, highlighting improvement projects which will target specific areas within the RF systems to ensure that adequate operating overheads are maintained and reliable operation is assured

  8. Analysis And Design Of A Water Purification System For The West African Area Of Operation

    Science.gov (United States)

    2016-12-01

    storage tank. Prior to activating the system, all the supply and drain lines were checked for leak and ensured they are secured. The water flow valve...gallons per Marine. Consequently, to supply water to 20,000 soldiers cost about $491,140 a day in Afghanistan (Lash 2011). In West Africa for example...the borehole water system (BWS), the source of water supply mostly used, lacks capability of monitoring chemical compound, filtration, and

  9. Quantum theory of the nonconservative system II

    International Nuclear Information System (INIS)

    Yeon, K.H.

    1984-01-01

    Utilizing the propagator for a damped harmonic oscillator in nonconservative system, we show the corresponding wave function, energy expectation value, transition amplitude and uncertainty relation. (Author)

  10. Rapid Automated Mission Planning System, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — The proposed innovation is an automated UAS mission planning system that will rapidly identify emergency (contingency) landing sites, manage contingency routing, and...

  11. Exergy Analysis of Air-Gap Membrane Distillation Systems for Water Purification Applications

    Directory of Open Access Journals (Sweden)

    Daniel Woldemariam

    2017-03-01

    Full Text Available Exergy analyses are essential tools for the performance evaluation of water desalination and other separation systems, including those featuring membrane distillation (MD. One of the challenges in the commercialization of MD technologies is its substantial heat demand, especially for large scale applications. Identifying such heat flows in the system plays a crucial role in pinpointing the heat loss and thermal integration potential by the help of exergy analysis. This study presents an exergetic evaluation of air-gap membrane distillation (AGMD systems at a laboratory and pilot scale. A series of experiments were conducted to obtain thermodynamic data for the water streams included in the calculations. Exergy efficiency and destruction for two different types of flat-plate AGMD were analyzed for a range of feed and coolant temperatures. The bench scale AGMD system incorporating condensation plate with more favorable heat conductivity contributed to improved performance parameters including permeate flux, specific heat demand, and exergy efficiency. For both types of AGMD systems, the contributions of the major components involved in exergy destruction were identified. The result suggested that the MD modules caused the highest fraction of destructions followed by re-concentrating tanks.

  12. Time, Temperature and Amount of Distilled Water Effects on the Purity and Yield of Bis(2-hydroxyethyl Terephthalate Purification System

    Directory of Open Access Journals (Sweden)

    H.W. Goh

    2015-07-01

    Full Text Available Polyethylene terephthalate (PET bottle is one of the common plastic wastes existed in the municipal solid waste in Malaysia. One alternative to solve the abundant of PET wastes is chemical recycling of the wastes to produce a value added product. This technology not only can decrease the PET wastes in landfill sites but also can produce many useful recycled PET products. Bis(2-hydroxyethyl terephthalate (BHET obtained from glycolysis reaction of PET waste was purified using crystallization process. The hot distilled water was added to glycolysis product followed by cooling and filtration to extract BHET in white solid form from the product. The effect of three operating conditions namely crystallization time, crystallization temperatures and amount of distilled water used to the yield of crystallization process were investigated. The purity of crystallization products were analyzed using HPLC and DSC. The optimum conditions of 3 hours crystallization time, 2 °C crystallization temperature and 5:1 mass ratio of distilled water used to glycolize solid gave the highest yield and purity of the crystallization process. © 2015 BCREC UNDIP. All rights reservedReceived: 12nd August 2014; Revised: 4th February 2015; Accepted: 5th February 2015How to Cite: Goh, H.W., Salmiaton, A., Abdullah, N., Idris, A. (2015. Time, Temperature and Amount of Distilled Water Effects on the Purity and Yield of Bis(2-hydroxyethyl Terephthalate Purification System. Bulletin of Chemical Reaction Engineering & Catalysis, 10 (2: 143-154. (doi:10.9767/bcrec.10.2.7195.143-154 Permalink/DOI: http://dx.doi.org/10.9767/bcrec.10.2.7195.143-154  

  13. Removal of Pb(II), Cu(II) and Cd(II) from aqueous solution by some fungi and natural adsorbents in single and multiple metal systems

    International Nuclear Information System (INIS)

    Shoaib, A.; Badar, T.; Aslam, N.

    2011-01-01

    Six fungal and 10 natural biosorbents were analyzed for their Cu(II), Cd(II) and Pb(II) uptake capacity from single, binary and ternary metal ion system. Preliminary screening biosorption of assays revealed 2 fungi (Aspergillus niger and Cunninghamella echinulata) and three natural [Cicer arietinum husk, Moringa oleifera flower and soil (clay)] adsorbents hold considerable high adsorption efficiency and capacity for 3 meta l ions amongst the adsorbents. Further biosorption trials with five elected adsorbents showed a considerable reduction in metal uptake capability of adsorbents in binary- and ternary systems as compared to singly metal system. Cd(II) manifested the highest inhibitory effect on the biosorption of other metal ions, followed by Pb(II) and Cu(II). On account of metal preference, the selectivity order for metal ion towards the studied biomass matrices was Pb(II) (40-90%) > Cd(II) (2-53%) > Cu(II) (2-30%). (author)

  14. Assessment of internal contamination problems associated with bioregenerative air/water purification systems

    Science.gov (United States)

    Johnson, Anne H.; Bounds, B. Keith; Gardner, Warren

    1990-01-01

    The emphasis is to characterize the mechanisms of bioregenerative revitalization of air and water as well as to assess the possible risks associated with such a system in a closed environment. Marsh and aquatic plants are utilized for purposes of wastewater treatment as well as possible desalinization and demineralization. Foliage plants are also being screened for their ability to remove toxic organics from ambient air. Preliminary test results indicate that treated wastewater is typically of potable quality with numbers of pathogens such as Salmonella and Shigella significantly reduced by the artificial marsh system. Microbiological analyses of ambient air indicate the presence of bacilli as well as thermophilic actinomycetes.

  15. Technical and economic aspects of purification strategies to minimise discharge water from companies with closed soilless cultivation systems

    NARCIS (Netherlands)

    Os, E.A. van; Bruins, M.; Beerling, E.; Jurgens, R.; Appelman, W.; Enthoven, N.

    2014-01-01

    The aim of the research project was to achieve closure by two complementary means: 1) maximising reuse of the nutrient solution by solving problems in recirculation that leads to discharge, and 2) purification of the left over discharged water. In this paper the technical and economic aspects of

  16. Technical and economic aspects of purification strategies to minimise discharge water from companies with closed soilless cultivation systems

    NARCIS (Netherlands)

    Os, van E.A.; Bruins, M.A.; Beerling, E.A.M.; Jurgens, R.; Appelman, W.; Enthoven, N.

    2014-01-01

    The aim of the research project was to achieve closure by two complementary means: 1) maximising reuse of the nutrient solution by solving problems in recircula-tion that leads to discharge, and 2) purification of the left over discharged water. In this paper the technical and economic aspects of

  17. An innovative attached-growth biological system for purification of pond water.

    Science.gov (United States)

    Chang, Chia-Yuan; Chang, Jing-Song; Chen, Chien-Min; Chiemchaisri, Chart; Vigneswaran, Saravanamuthu

    2010-03-01

    This study applied the non-woven material from used diaper as the carrier for bio-film process to purify the recycled water from a landscape pond at the Tainan City Municipal Culture Center (TCMCC), Taiwan. An on-site system was installed and the experiment was accomplished through three stages in 192 days with different time periods of 70 days, 63 days, and 59 days, respectively. The results showed that the non-woven media is functional for SS removal. The average SS removal of stages 1, 2, and 3 were 91%, 96%, and 95%, respectively. The highest SCOD removal efficiency of 90% occurred at stage 3. A significant color improvement of the pond water was achieved through this non-woven bio-carrier treatment system. Whole system can be without any maintenance for 139 days. The result indicated that the non-woven medium system was with a great potential in treating and recycling the pond water with stable operation and satisfactory removal performance. 2009 Elsevier Ltd. All rights reserved.

  18. Purification of the gas after pyrolysis in coupled plasma-catalytic system

    Directory of Open Access Journals (Sweden)

    Młotek Michał

    2017-12-01

    Full Text Available Gliding discharge and coupled plasma-catalytic system were used for toluene conversion in a gas composition such as the one obtained during pyrolysis of biomass. The chosen catalyst was G-0117, which is an industrial catalyst for methane conversion manufactured by INS Pulawy (Poland. The effects of discharge power, initial concentration of toluene, gas flow rate and the presence of the bed of the G-0117 catalyst on the conversion of C7H8, a model tars compounds were investigated. Conversion of coluene increases with discharge power and the highest one was noted in the coupled plasma-catalytic system. It was higher than that in the homogeneous system of gliding discharge. When applying a reactor with reduced G-0117 and CO (0.15 mol%, CO2 (0.15 mol%, H2 (0.30 mol%, N2 (0.40 mol%, 4000 ppm of toluene and gas flow rate of 1.5 Nm3/h, the conversion of toluene was higher than 99%. In the coupled plasma-catalytic system with G-0117 methanation of carbon oxides was observed.

  19. Fusion fuel purification during the Tritium Systems Test Assembly 3-week loop experiment

    International Nuclear Information System (INIS)

    Willms, R.S.

    1989-01-01

    During the time period from April 19, 1989--May 5, 1989, the Tritium Systems Test Assembly (TSTA) at Los Alamos National Laboratory (LANL) conducted its longest continuous integrated loop operation to date. This provided an opportunity to test some hitherto unproven capabilities of the TSTA Fuel Cleanup System (FCU). Previous FCU tests were reported. The purpose of the FCU is to remove impurities from a stream of hydrogen isotopes (Q 2 ) representative of torus exhaust gas. During this run impurities loadings ranging from 60 to 179 sccm of 90% N 2 and 10% CH 4 were fed to the FCU. Each of the two FCU main flow molecular sieve beds (MSB's) were filled to breakthrough three times. The MSB's were regenerated during loop operations. 2 refs., 6 figs., 2 tabs

  20. Electronic construction collaboration system -- phase II.

    Science.gov (United States)

    2010-06-01

    During the first year of research, work was completed to identify Iowa DOT needs for web-based project management system (WPMS) : and evaluate how commercially available solutions could meet these needs. Researchers also worked to pilot test custom d...

  1. Mars Aqueous Processing System, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — The Mars Aqueous Processing System (MAPS) is a novel technology for recovering oxygen, iron, and other constituents from lunar and Mars soils. The closed-loop...

  2. Wearable Health Monitoring Systems, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — The objective of this proposal is to demonstrate the feasibility of producing a wearable health monitoring system for the human body that is functional, comfortable,...

  3. Tactile Data Entry System, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — Building on our successful Phase I Tactile Data Entry program, Barron Associates proposes development of a Glove-Enabled Computer Operations (GECO) system to permit...

  4. Advanced Green Micropropulsion System, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — Systima in collaboration with University of Washington is developing a high performance injection system for advanced green monopropellant AF-M315E micropropulsion...

  5. Enhanced Brine Dewatering System, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — The purpose of the Enhanced Brine Dewatering System (EBDS) is to provide a scalable means of completely recovering usable water from byproducts created by reverse...

  6. Nanolipoprotein particles and related methods and systems for protein capture, solubilization, and/or purification

    Energy Technology Data Exchange (ETDEWEB)

    Chromy, Brett A.; Henderson, Paul; Hoeprich, Jr, Paul D.

    2016-10-04

    Provided herein are methods and systems for assembling, solubilizing and/or purifying a membrane associated protein in a nanolipoprotein particle, which comprise a temperature transition cycle performed in presence of a detergent, wherein during the temperature transition cycle the nanolipoprotein components are brought to a temperature above and below the gel to liquid crystalling transition temperature of the membrane forming lipid of the nanolipoprotein particle.

  7. A Novel Aqueous Two Phase System Composed of Surfactant and Xylitol for the Purification of Lipase from Pumpkin (Cucurbita moschata) Seeds and Recycling of Phase Components.

    Science.gov (United States)

    Amid, Mehrnoush; Manap, Mohd Yazid; Hussin, Muhaini; Mustafa, Shuhaimi

    2015-06-17

    Lipase is one of the more important enzymes used in various industries such as the food, detergent, pharmaceutical, textile, and pulp and paper sectors. A novel aqueous two-phase system composed of surfactant and xylitol was employed for the first time to purify lipase from Cucurbita moschata. The influence of different parameters such as type and concentration of surfactants, and the composition of the surfactant/xylitol mixtures on the partitioning behavior and recovery of lipase was investigated. Moreover, the effect of system pH and crude load on the degree of purification and yield of the purified lipase were studied. The results indicated that the lipase was partitioned into the top surfactant rich phase while the impurities partitioned into the bottom xylitol-rich phase using an aqueous two phase system composed of 24% (w/w) Triton X-100 and 20% (w/w) xylitol, at 56.2% of tie line length (TLL), (TTL is one of the important parameters in this study and it is determined from a bimodal curve in which the tie-line connects two nodes on the bimodal, that represent concentration of phase components in the top and bottom phases) and a crude load of 25% (w/w) at pH 8.0. Recovery and recycling of components was also measured in each successive step process. The enzyme was successfully recovered by the proposed method with a high purification factor of 16.4 and yield of 97.4% while over 97% of the phase components were also recovered and recycled. This study demonstrated that the proposed novel aqueous two phase system method is more efficient and economical than the traditional aqueous two phase system method for the purification and recovery of the valuable enzyme lipase.

  8. A Novel Aqueous Two Phase System Composed of Surfactant and Xylitol for the Purification of Lipase from Pumpkin (Cucurbita moschata Seeds and Recycling of Phase Components

    Directory of Open Access Journals (Sweden)

    Mehrnoush Amid

    2015-06-01

    Full Text Available Lipase is one of the more important enzymes used in various industries such as the food, detergent, pharmaceutical, textile, and pulp and paper sectors. A novel aqueous two-phase system composed of surfactant and xylitol was employed for the first time to purify lipase from Cucurbita moschata. The influence of different parameters such as type and concentration of surfactants, and the composition of the surfactant/xylitol mixtures on the partitioning behavior and recovery of lipase was investigated. Moreover, the effect of system pH and crude load on the degree of purification and yield of the purified lipase were studied. The results indicated that the lipase was partitioned into the top surfactant rich phase while the impurities partitioned into the bottom xylitol-rich phase using an aqueous two phase system composed of 24% (w/w Triton X-100 and 20% (w/w xylitol, at 56.2% of tie line length (TLL, (TTL is one of the important parameters in this study and it is determined from a bimodal curve in which the tie-line connects two nodes on the bimodal, that represent concentration of phase components in the top and bottom phases and a crude load of 25% (w/w at pH 8.0. Recovery and recycling of components was also measured in each successive step process. The enzyme was successfully recovered by the proposed method with a high purification factor of 16.4 and yield of 97.4% while over 97% of the phase components were also recovered and recycled. This study demonstrated that the proposed novel aqueous two phase system method is more efficient and economical than the traditional aqueous two phase system method for the purification and recovery of the valuable enzyme lipase.

  9. HYLIFE-II tritium management system

    International Nuclear Information System (INIS)

    Longhurst, G.R.; Dolan, T.J.

    1993-06-01

    The tritium management system performs seven functions: (1) tritium gas removal from the blast chamber, (2) tritium removal from the Flibe, (3) tritium removal from helium sweep gas, (4) tritium removal from room air, (5) hydrogen isotope separation, (6) release of non-hazardous gases through the stack, (7) fixation and disposal of hazardous effluents. About 2 TBq/s (5 MCi/day) of tritium is bred in the Flibe (Li 2 BeF 4 ) molten salt coolant by neutron absorption. Tritium removal is accomplished by a two-stage vacuum disengager in each of three steam generator loops. Each stage consists of a spray of 0.4 mm diameter, hot Flibe droplets into a vacuum chamber 4 m in diameter and 7 m tall. As droplets fall downward into the vacuum, most of the tritium diffuses out and is pumped away. A fraction Φ∼10 -5 of the tritium remains in the Flibe as it leaves the second stage of the vacuum disengager, and about 24% of the remaining tritium penetrates through the steam generator tubes, per pass, so the net leakage into the steam system is about 4.7 MBq/s (11 Ci/day). The required Flibe pumping power for the vacuum disengager system is 6.6 MW. With Flibe primary coolant and a vacuum disengager, an intermediate coolant loop is not needed to prevent tritium from leaking into the steam system. An experiment is needed to demonstrate vacuum disengager operation with Flibe. A secondary containment shell with helium sweep gas captures the tritium permeating out of the Flibe ducts, limiting leaks there to about 1 Ci/day. The tritium inventory in the reactor is about 190 g, residing mostly in the large Flibe recirculation duct walls. The total cost of the tritium management system is 92 M$, of which the vacuum disengagers cost = 56%, the blast chamber vacuum system = 15%, the cryogenic plant = 9%, the emergency air cleanup and waste treatment systems each = 6%, the protium removal system = 3%, and the fuel storage system and inert gas system each = 2%

  10. Detection of criticality accidents. The Intertechnique EDAC II system

    International Nuclear Information System (INIS)

    Prigent, R.

    1991-01-01

    The chief aim of the new generation of EDAC II criticality accidents detection system is to reduce the risks associated to the handling of fissile material by providing a swift and safe warning of the development of any criticality accident. To this function already devolving on the EDAC system of the previous generation, the EDAC II adds the possibility of storing in memory the characteristics of the accident, providing a daily follow-up of the striking events in the system through the print-out of a log book and providing assistance to the operators during the periodical tests. (Author)

  11. Strep-Tagged Protein Purification.

    Science.gov (United States)

    Maertens, Barbara; Spriestersbach, Anne; Kubicek, Jan; Schäfer, Frank

    2015-01-01

    The Strep-tag system can be used to purify recombinant proteins from any expression system. Here, protocols for lysis and affinity purification of Strep-tagged proteins from E. coli, baculovirus-infected insect cells, and transfected mammalian cells are given. Depending on the amount of Strep-tagged protein in the lysate, a protocol for batch binding and subsequent washing and eluting by gravity flow can be used. Agarose-based matrices with the coupled Strep-Tactin ligand are the resins of choice, with a binding capacity of up to 9 mg ml(-1). For purification of lower amounts of Strep-tagged proteins, the use of Strep-Tactin magnetic beads is suitable. In addition, Strep-tagged protein purification can also be automated using prepacked columns for FPLC or other liquid-handling chromatography instrumentation, but automated purification is not discussed in this protocol. The protocols described here can be regarded as an update of the Strep-Tag Protein Handbook (Qiagen, 2009). © 2015 Elsevier Inc. All rights reserved.

  12. Liquid argon dielectric breakdown studies with the MicroBooNE purification system

    Energy Technology Data Exchange (ETDEWEB)

    Acciarri, R.; Carls, B.; James, C.; Johnson, B.; Jostlein, H.; Lockwitz, S.; Lundberg, B.; Raaf, J. L.; Rameika, R.; Rebel, B.; Zeller, G. P.; Zuckerbrot, M.

    2014-11-01

    The proliferation of liquid argon time projection chamber detectors makes the characterization of the dielectric properties of liquid argon a critical task. To improve understanding of these properties, a systematic study of the breakdown electric field in liquid argon was conducted using a dedicated cryostat connected to the MicroBooNE cryogenic system at Fermilab. An electrode sphere-plate geometry was implemented using spheres with diameters of 1.3 mm, 5.0 mm, and 76 mm. The MicroBooNE cryogenic system allowed measurements to be taken at a variety of electronegative contamination levels ranging from a few parts-per-million to tens of parts-per-trillion. The cathode-anode distance was varied from 0.1 mm to 2.5 cm. The results demonstrate a geometric dependence of the electric field strength at breakdown. This study is the first time that the dependence of the breakdown field on stressed cathode area has been shown for liquid argon.

  13. Sensitive detection of biothiols and histidine based on the recovered fluorescence of the carbon quantum dots–Hg(II) system

    Energy Technology Data Exchange (ETDEWEB)

    Hou, Juan; Zhang, Fengshuang; Yan, Xu; Wang, Long; Yan, Jin [College of Chemistry, Jilin University, 2699 Qianjin Street, Changchun 130012 (China); Ding, Hong [State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of Chemistry, Jilin University, Changchun 130012 (China); Ding, Lan, E-mail: dinglan@jlu.edu.cn [College of Chemistry, Jilin University, 2699 Qianjin Street, Changchun 130012 (China)

    2015-02-15

    Highlights: • Carbon quantum dots-based probe was used for detection of GSH, Cys or His. • The fluorescence of CQDs was quenched by Hg(II) and then recovered by GSH, Cys or His. • No further surface modification or purification of CQDs was required. • This sensor exhibits superior accuracy and sensitivity. • The proposed method was simple in design, fast in operation. - Abstract: In this paper, we presented a novel, rapid and highly sensitive sensor for glutathione (GSH), cysteine (Cys) and histidine (His) based on the recovered fluorescence of the carbon quantum dots (CQDs)–Hg(II) system. The CQDs were synthesized by microwave-assisted approach in one pot according to our previous report. The fluorescence of CQDs could be quenched in the presence of Hg(II) due to the coordination occurring between Hg(II) and functional groups on the surface of CQDs. Subsequently, the fluorescence of the CQDs–Hg(II) system was recovered gradually with the addition of GSH, Cys or His due to their stronger affinity with Hg(II). A good linear relationship was obtained from 0.10 to 20 μmol L{sup −1} for GSH, from 0.20 to 45 μmol L{sup −1} for Cys and from 0.50 to 60 μmol L{sup −1} for His, respectively. This method has been successfully applied to the trace detection of GSH, Cys or His in human serum samples with satisfactory results. The proposed method was simple in design and fast in operation, which demonstrated great potential in bio-sensing fields.

  14. Sensitive detection of biothiols and histidine based on the recovered fluorescence of the carbon quantum dots–Hg(II) system

    International Nuclear Information System (INIS)

    Hou, Juan; Zhang, Fengshuang; Yan, Xu; Wang, Long; Yan, Jin; Ding, Hong; Ding, Lan

    2015-01-01

    Highlights: • Carbon quantum dots-based probe was used for detection of GSH, Cys or His. • The fluorescence of CQDs was quenched by Hg(II) and then recovered by GSH, Cys or His. • No further surface modification or purification of CQDs was required. • This sensor exhibits superior accuracy and sensitivity. • The proposed method was simple in design, fast in operation. - Abstract: In this paper, we presented a novel, rapid and highly sensitive sensor for glutathione (GSH), cysteine (Cys) and histidine (His) based on the recovered fluorescence of the carbon quantum dots (CQDs)–Hg(II) system. The CQDs were synthesized by microwave-assisted approach in one pot according to our previous report. The fluorescence of CQDs could be quenched in the presence of Hg(II) due to the coordination occurring between Hg(II) and functional groups on the surface of CQDs. Subsequently, the fluorescence of the CQDs–Hg(II) system was recovered gradually with the addition of GSH, Cys or His due to their stronger affinity with Hg(II). A good linear relationship was obtained from 0.10 to 20 μmol L −1 for GSH, from 0.20 to 45 μmol L −1 for Cys and from 0.50 to 60 μmol L −1 for His, respectively. This method has been successfully applied to the trace detection of GSH, Cys or His in human serum samples with satisfactory results. The proposed method was simple in design and fast in operation, which demonstrated great potential in bio-sensing fields

  15. Criteria for selecting a flue gas purification system for waste incineration plants

    Energy Technology Data Exchange (ETDEWEB)

    Mosch, H

    1985-12-01

    This paper evaluates the available systems with a view to three basic principles of gaseous effluent removal, relating to dust, SO/sub 2/, HCl and HF, by discussing criteria such as performance with regard to environmental hygiene, performance with regard to material or energy consumption, terms and conditions, operational conditions, and economics. The three methods discussed are: (1) Scrubbing and effluent treatment including evaporation and flue gas reheating or other means of treating the flue gas cooled down to about 65/sup 0/C. (2) Spray sorption by means of flash drying reactors, similar to the spray drying method. (3) Blow-in sorption, as the method may be called. The dry, powdered reagent, in general slack lime with at least 90 p.c. of Ca(OH)/sub 2/, is blown into the reactors. (orig./HP).

  16. Complement System Part II: Role in Immunity

    Science.gov (United States)

    Merle, Nicolas S.; Noe, Remi; Halbwachs-Mecarelli, Lise; Fremeaux-Bacchi, Veronique; Roumenina, Lubka T.

    2015-01-01

    The complement system has been considered for a long time as a simple lytic cascade, aimed to kill bacteria infecting the host organism. Nowadays, this vision has changed and it is well accepted that complement is a complex innate immune surveillance system, playing a key role in host homeostasis, inflammation, and in the defense against pathogens. This review discusses recent advances in the understanding of the role of complement in physiology and pathology. It starts with a description of complement contribution to the normal physiology (homeostasis) of a healthy organism, including the silent clearance of apoptotic cells and maintenance of cell survival. In pathology, complement can be a friend or a foe. It acts as a friend in the defense against pathogens, by inducing opsonization and a direct killing by C5b–9 membrane attack complex and by triggering inflammatory responses with the anaphylatoxins C3a and C5a. Opsonization plays also a major role in the mounting of an adaptive immune response, involving antigen presenting cells, T-, and B-lymphocytes. Nevertheless, it can be also an enemy, when pathogens hijack complement regulators to protect themselves from the immune system. Inadequate complement activation becomes a disease cause, as in atypical hemolytic uremic syndrome, C3 glomerulopathies, and systemic lupus erythematosus. Age-related macular degeneration and cancer will be described as examples showing that complement contributes to a large variety of conditions, far exceeding the classical examples of diseases associated with complement deficiencies. Finally, we discuss complement as a therapeutic target. PMID:26074922

  17. A New Clinical HIFU System (Teleson II)

    Science.gov (United States)

    Ma, Yixin; Symonds-Tayler, Richard; Rivens, Ian H.; ter Haar, Gail R.

    2007-05-01

    Previous clinical trials with our first prototype HIFU system (Teleson I) for the treatment of liver tumors, demonstrated a major challenge to be treatment of those tumors located behind the ribs. We have designed a new multi-element transducer for rib sparing. Initial simulation and experimental results (using a single channel power amplifier) are very encouraging. A new clinical HIFU system which can drive the multi-element transducer and control each channel independently is being designed and constructed. This second version of a clinical prototype HIFU system consists of a 3D motorised gantry, a multi-channel signal generator, a multi-channel power amplifier, a user interface PC, an embedded controller and auxiliary circuits for real-time interleaving/synchronization control and a to-be-implemented safety monitoring and data logging unit. For multi-element transducers, each element can be individually switched on and off for rib sparing, and phase and amplitude modulated for potential phased array applications. The multi-channel power amplifier can be switched on/off very rapidly at required intervals to interleave with ultrasound B-Scan imaging for HIFU monitoring or radiation force elastography imaging via a dedicated interleaving/timing module. The gantry movement can also be synchronised with power amplifier on/off and phase/amplitude updating for lesion generation under a wide variety of conditions including single lesions, lesion arrays and lesions "tracks" created whilst translating the active transducer. Results from testing the system using excised tissue will be presented.

  18. Air purification from TCE and PCE contamination in a hybrid bioreactors and biofilter integrated system.

    Science.gov (United States)

    Tabernacka, Agnieszka; Zborowska, Ewa; Lebkowska, Maria; Borawski, Maciej

    2014-01-15

    A two-stage waste air treatment system, consisting of hybrid bioreactors (modified bioscrubbers) and a biofilter, was used to treat waste air containing chlorinated ethenes - trichloroethylene (TCE) and tetrachloroethylene (PCE). The bioreactor was operated with loadings in the range 0.46-5.50gm(-3)h(-1) for TCE and 2.16-9.02gm(-3)h(-1) for PCE. The biofilter loadings were in the range 0.1-0.97gm(-3)h(-1) for TCE and 0.2-2.12gm(-3)h(-1) for PCE. Under low pollutant loadings, the efficiency of TCE elimination was 23-25% in the bioreactor and 54-70% in the biofilter. The efficiency of PCE elimination was 44-60% in the bioreactor and 50-75% in the biofilter. The best results for the bioreactor were observed one week after the pollutant loading was increased. However, the process did not stabilize. In the next seven days contaminant removal efficiency, enzymatic activity and biomass content were all diminished. Copyright © 2013 Elsevier B.V. All rights reserved.

  19. Expression and Purification of C-Peptide Containing Insulin Using Pichia pastoris Expression System

    Directory of Open Access Journals (Sweden)

    Mohammed N. Baeshen

    2016-01-01

    Full Text Available Increase in the incidence of Insulin Dependent Diabetes Mellitus (IDDM among people from developed and developing countries has created a large global market for insulin. Moreover, exploration of new methods for insulin delivery including oral or inhalation route which require very high doses would further increase the demand of cost-effective recombinant insulin. Various bacterial and yeast strains have been optimized to overproduce important biopharmaceuticals. One of the approaches we have taken is the production of recombinant human insulin along with C-peptide in yeast Pichia pastoris. We procured a cDNA clone of insulin from Origene Inc., USA. Insulin cDNA was PCR amplified and cloned into yeast vector pPICZ-α. Cloned insulin cDNA was confirmed by restriction analysis and DNA sequencing. pPICZ-α-insulin clone was transformed into Pichia pastoris SuperMan5 strain. Several Zeocin resistant clones were obtained and integration of insulin cDNA in Pichia genome was confirmed by PCR using insulin specific primers. Expression of insulin in Pichia clones was confirmed by ELISA, SDS-PAGE, and Western blot analysis. In vivo efficacy studies in streptozotocin induced diabetic mice confirmed the activity of recombinant insulin. In conclusion, a biologically active human proinsulin along with C-peptide was expressed at high level using Pichia pastoris expression system.

  20. Study on magnetic separation system using high Tc superconducting bulk magnets for water purification technique

    International Nuclear Information System (INIS)

    Oka, T; Kanayama, H; Tanaka, K; Fukui, S; Ogawa, J; Sato, T; Ooizumi, M; Yamaguchi, M; Yokoyama, K; Noto, K

    2009-01-01

    The application of superconducting bulk magnets to the magnetic separation techniques has been investigated for the Mn-bearing waste water drained from the university laboratories. The research has been conducted in comparison with the electromagnets, and the cryo-cooled superconducting solenoid magnet. The separation ratios of ferrite precipitates including Mn element in the waste slurry were estimated by means of the high gradient magnetic separation method with ferromagnetic iron filters in the water channel and open gradient magnetic separation without them. As the magnetic force acting on the particles is given by the product of a magnetization of particles and a gradient of magnetic field, and a superconducting bulk magnet shows a sharp gradient of the magnetic field on the surface, the performances of the bulk magnet system were almost equivalent to those of the superconducting solenoid magnet with wide bore with respect to the magnetic separation ratios. The separation ratios for Mn have reached over 80 % for HGMS and 10 % for OGMS under the flow rates less than 3 liter/min.

  1. Overview of the TJ-II remote participation system

    International Nuclear Information System (INIS)

    Vega, J.; Sanchez, E.; Portas, A.; Pereira, A.; Mollinedo, A.; Munoz, J.A.; Ruiz, M.; Barrera, E.; Lopez, S.; Machon, D.; Castro, R.; Lopez, D.

    2006-01-01

    The TJ-II remote participation system (RPS) is focused on providing remote access to elements that depend exclusively on characteristics of the TJ-II environment: data acquisition, diagnostics control systems and TJ-II operation tracking. Four key points were taken into account prior to starting the software design: access security, software execution platforms, software maintenance and distribution and delivery of operation events. The first, access security, was addressed by means of a distributed authentication and authorization system, PAPI. Regarding the other points, the development was based on the use of web servers (due to their standard character, flexibility and scalability) and Java technologies (due to their open nature, security properties and technological maturity). Software deployment was prepared to make use of the Java Network Launching Protocol (JNLP). On-line message distribution was planned according to a message oriented middleware. At present, the TJ-II RPS manages over 1000 digitization channels and 20 diagnostic control systems. The TJ-II RPS architecture is flexible, scalable and powerful enough to be applied to distributed environments and, in particular, it could be used in the ITER environment

  2. Overview of the TJ-II remote participation system

    Energy Technology Data Exchange (ETDEWEB)

    Vega, J. [Asociacion EURATOM/CIEMAT para Fusion, Avda. Complutense, 22, 28040 Madrid (Spain)]. E-mail: jesus.vega@ciemat.es; Sanchez, E. [Asociacion EURATOM/CIEMAT para Fusion, Avda. Complutense, 22, 28040 Madrid (Spain); Portas, A. [Asociacion EURATOM/CIEMAT para Fusion, Avda. Complutense, 22, 28040 Madrid (Spain); Pereira, A. [Asociacion EURATOM/CIEMAT para Fusion, Avda. Complutense, 22, 28040 Madrid (Spain); Mollinedo, A. [Computer Centre, CIEMAT, Avda. Complutense, 22, 28040 Madrid (Spain); Munoz, J.A. [Computer Centre, CIEMAT, Avda. Complutense, 22, 28040 Madrid (Spain); Ruiz, M. [Dpto. De Sistemas Electronicos y de Control, UPM, Campus Sur, Ctra. Valencia km 7, 28031 Madrid (Spain); Barrera, E. [Dpto. De Sistemas Electronicos y de Control, UPM, Campus Sur, Ctra. Valencia km 7, 28031 Madrid (Spain); Lopez, S. [Dpto. De Sistemas Electronicos y de Control, UPM, Campus Sur, Ctra. Valencia km 7, 28031 Madrid (Spain); Machon, D. [Dpto. De Sistemas Electronicos y de Control, UPM, Campus Sur, Ctra. Valencia km 7, 28031 Madrid (Spain); Castro, R. [Red.es-RedIRIS, Edificio Bronce, Plaza Manuel Gomez Moreno s/n, 28020 Madrid (Spain); Lopez, D. [Red.es-RedIRIS, Edificio Bronce, Plaza Manuel Gomez Moreno s/n, 28020 Madrid (Spain)

    2006-07-15

    The TJ-II remote participation system (RPS) is focused on providing remote access to elements that depend exclusively on characteristics of the TJ-II environment: data acquisition, diagnostics control systems and TJ-II operation tracking. Four key points were taken into account prior to starting the software design: access security, software execution platforms, software maintenance and distribution and delivery of operation events. The first, access security, was addressed by means of a distributed authentication and authorization system, PAPI. Regarding the other points, the development was based on the use of web servers (due to their standard character, flexibility and scalability) and Java technologies (due to their open nature, security properties and technological maturity). Software deployment was prepared to make use of the Java Network Launching Protocol (JNLP). On-line message distribution was planned according to a message oriented middleware. At present, the TJ-II RPS manages over 1000 digitization channels and 20 diagnostic control systems. The TJ-II RPS architecture is flexible, scalable and powerful enough to be applied to distributed environments and, in particular, it could be used in the ITER environment.

  3. MRI findings in central nervous system of neurofibromatosis-II

    International Nuclear Information System (INIS)

    Chen Maoen; Huang Suiqiao; Shen Jun; Hong Guobin; Wu Zhuo; Lin Xiaofeng

    2007-01-01

    Objective: To investigate the diagnostic value of MR imaging in central nervous system involvement of neurofibromatosis II. Methods: 7 patients with surgically and pathologically proved neurofibromatosis II were included. Their MR imaging findings and clinical features were retrospectively analyzed. Results: The main findings of 7 cases of neurofibraomaosis II on MR imaging included bilateral acoustic neurilemoma, multiple neurofibroma, meningioma and schwannoma. Among the 7 patients, Tl-weighted imaging after contrast enhancement displayed additional lesions which had been ignored on un-enhanced scan. Conclusion: MR imaging has advantages in the detection of central nervous sys- tem involvement of neurofibromatosis II with regard to its ability to show the lesions well, meanwhile displaying the size, morphology and signal features clearly. (authors)

  4. Design and Implementation of an Embedded NIOS II System for JPEG2000 Tier II Encoding

    Directory of Open Access Journals (Sweden)

    John M. McNichols

    2013-01-01

    Full Text Available This paper presents a novel implementation of the JPEG2000 standard as a system on a chip (SoC. While most of the research in this field centers on acceleration of the EBCOT Tier I encoder, this work focuses on an embedded solution for EBCOT Tier II. Specifically, this paper proposes using an embedded softcore processor to perform Tier II processing as the back end of an encoding pipeline. The Altera NIOS II processor is chosen for the implementation and is coupled with existing embedded processing modules to realize a fully embedded JPEG2000 encoder. The design is synthesized on a Stratix IV FPGA and is shown to out perform other comparable SoC implementations by 39% in computation time.

  5. Inactive trials of transport systems: phase II

    International Nuclear Information System (INIS)

    Haberlin, M.M.; Hardy, A.R.; Kennedy, S.T.

    1986-11-01

    Progress made during 1984-85 is reviewed in four sections: the design and installation of a stainless steel working floor in the mock-up of a crate handling and size reduction facility; the detailed evaluation of a single air pad of the type used on commercial air-transporter; an experimental programme designed to examine the problems associated with the operation of a commercial air-transporter; the design, manufacture and commissioning trials of two powered conveyor units which when combined complete a remotely operated transfer system for transporting crated waste into and within the mock-up facility. (author)

  6. Dielectric susceptibility of classical Coulomb systems. II

    International Nuclear Information System (INIS)

    Choquard, Ph.; Piller, B.; Rentsch, R.

    1987-01-01

    This paper deals with the shape dependence of the dielectric susceptibility (equivalently defined, in a canonical ensemble, by the mean square fluctuation of the electric polarization or by the second moment of the charge-charge correlation function) of classical Coulomb systems. The concept of partial second moment is introduced with the aim of analyzing the contributions to the total susceptibility of pairs of particles of increasing separation. For a disk-shaped one-component plasma with coupling parameter γ=2 it is shown, numerically and algebraically for small and large systems, that (1) the correlation function of two particles close to the edge of the disk decays as the inverse of the square of their distance, and (2) the susceptibility is made up of a bulk contribution, which saturates rapidly toward the Stillinger-Lovett value, and of surface contribution, which varies on the scale of the disk diameter and is described by a new law called the arc sine law. It is also shown that electrostatics and statistical mechanics with shape-dependent thermodynamic limits are consistent for the same model in a strip geometry, whereas the Stillinger-Lovett sum rule is verified for a boundary-free geometry such as the surface of a sphere. Some results of extensive computer simulations of one- and two-component plasmas in circular and elliptic geometries are shown. Anisotropy effects on the susceptibilities are clearly demonstrated and the arc sine law for a circular plasma is well confirmed

  7. Bioinspired Materials for Water Purification

    Directory of Open Access Journals (Sweden)

    Alfredo Gonzalez-Perez

    2016-06-01

    Full Text Available Water scarcity issues associated with inadequate access to clean water and sanitation is a ubiquitous problem occurring globally. Addressing future challenges will require a combination of new technological development in water purification and environmental remediation technology with suitable conservation policies. In this scenario, new bioinspired materials will play a pivotal role in the development of more efficient and environmentally friendly solutions. The role of amphiphilic self-assembly on the fabrication of new biomimetic membranes for membrane separation like reverse osmosis is emphasized. Mesoporous support materials for semiconductor growth in the photocatalytic degradation of pollutants and new carriers for immobilization of bacteria in bioreactors are used in the removal and processing of different kind of water pollutants like heavy metals. Obstacles to improve and optimize the fabrication as well as a better understanding of their performance in small-scale and pilot purification systems need to be addressed. However, it is expected that these new biomimetic materials will find their way into the current water purification technologies to improve their purification/removal performance in a cost-effective and environmentally friendly way.

  8. WARRIOR II, a high performance modular electric robot system

    International Nuclear Information System (INIS)

    Downton, G.C.

    1996-01-01

    Initially designed for in-reactor welding by the Central Electricity Generating Board, WARRIOR has been developed using the concept of modular technology to become a light-weight, high performance robotic system. Research work on existing machines for in-reactor inspection and repair and heavy duty hydraulic manipulators was progressed in order to develop WARRIOR II, a versatile in-reactor welding system usable at any nuclear power station light enough to be deployed by existing remote handling equipment. WARRIOR II can be significantly reconfigured quickly to pursue different ends. (UK)

  9. Physical and chemical quality, biodiversity, and thermodynamic prediction of adhesion of bacterial isolates from a water purification system: a case study

    Directory of Open Access Journals (Sweden)

    Roberta Barbosa Teodoro Alves

    2017-06-01

    Full Text Available ABSTRACT The objective of this study was to evaluate the quality of water purification system and identify the bacteria this system, predict bacterial adherence according to the hydrophobicity of these microorganisms and of the polypropylene distribution loop for purified water. The assessment of drinking water that supplies the purification system allowed good-quality physical, chemical, and microbiological specifications. The physicochemical specifications of the distributed purified water were approved, but the heterotrophic bacteria count was higher than allowed (>2 log CFU mL-1.The sanitation of the storage tank with chlorine decreased the number of bacteria adhered to the surface (4.34 cycles log. By sequencing of the 16SrDNA genes, six species of bacteria were identified. The contact angle was determined and polypropylene surface and all bacteria were considered to be hydrophilic, and adhesion was thermodynamically unfavorable. This case study showed the importance of monitoring the water quality in the purified water systems and the importance of sanitization with chemical agents. The count of heterotrophic bacteria on the polypropylene surface was consistent with the predicted thermodynamics results because the number of adhered cells reached approximate values of 5 log CFU cm-2.

  10. Centrifugal LabTube platform for fully automated DNA purification and LAMP amplification based on an integrated, low-cost heating system.

    Science.gov (United States)

    Hoehl, Melanie M; Weißert, Michael; Dannenberg, Arne; Nesch, Thomas; Paust, Nils; von Stetten, Felix; Zengerle, Roland; Slocum, Alexander H; Steigert, Juergen

    2014-06-01

    This paper introduces a disposable battery-driven heating system for loop-mediated isothermal DNA amplification (LAMP) inside a centrifugally-driven DNA purification platform (LabTube). We demonstrate LabTube-based fully automated DNA purification of as low as 100 cell-equivalents of verotoxin-producing Escherichia coli (VTEC) in water, milk and apple juice in a laboratory centrifuge, followed by integrated and automated LAMP amplification with a reduction of hands-on time from 45 to 1 min. The heating system consists of two parallel SMD thick film resistors and a NTC as heating and temperature sensing elements. They are driven by a 3 V battery and controlled by a microcontroller. The LAMP reagents are stored in the elution chamber and the amplification starts immediately after the eluate is purged into the chamber. The LabTube, including a microcontroller-based heating system, demonstrates contamination-free and automated sample-to-answer nucleic acid testing within a laboratory centrifuge. The heating system can be easily parallelized within one LabTube and it is deployable for a variety of heating and electrical applications.

  11. Control system for the Spanish Stellarator TJ-II

    International Nuclear Information System (INIS)

    Pacios, L.; Blaumoser, M.; Pena, A. de la; Carrasco, R.; Labrador, I.; Lapayese, F.; Diaz, J.C.; Laso, L.M.

    1995-01-01

    We describe the distributed control and monitoring system for the Spanish Stellarator TJ-II, which is under construction at CIEMAT in Madrid. It consists of one central UNIX workstation and several autonomous subsystems based on VME crates with embedded processors under OS-9 real-time operating system and PLCs. The system integrates the machine and discharge control. An operator can perform the control and plasma discharge by means of a user-friendly graphic interface. (orig.)

  12. EBR-II water-to-sodium leak detection system

    International Nuclear Information System (INIS)

    Wrightson, M.M.; McKinley, K.; Ruther, W.E.; Holmes, J.T.

    1976-01-01

    The water-to-sodium leak detection system installed at EBR-II in April, 1975, is described in detail. Topics covered include operational characteristics, maintenance problems, alarm functions, background hydrogen level data, and future plans for refinements to the system. Particular emphasis is given to the failures of eight of the ten leak detectors due to sodium-to-vacuum leakage, and the program anticipated for complete recovery of the system

  13. Mark-II Data Acquisition and Trigger system

    International Nuclear Information System (INIS)

    Breidenbach, M.

    1984-06-01

    The Mark-II Data Acquisition and Trigger system requirements and general solution are described. The solution takes advantage of the synchronous crossing times and low event rates of an electron positron collider to permit a very highly multiplexed analog scheme to be effective. The system depends on a two level trigger to operate with acceptable dead time. The trigger, multiplexing, data reduction, calibration, and CAMAC systems are described

  14. Data systems in FFTF and EBR-II

    International Nuclear Information System (INIS)

    Warrick, R.P.; Ritter, W.M.

    1980-02-01

    This paper describes the Data System used to monitor operation and collect experimental data in FFTF. This data system has evolved since initial inception from a relatively simple, single computer system monitoring a relatively few (approx. 1000) instrument channels important for operation to one which has increased capability to support the long-range testing needs in FFTF. The system, while still relatively simple, now contains multiple computers which normally perform independent functions. The computers, however, provide backup processing for certain simple tasks. Operator interfacing is provided through CRT's. The output capabilities of the system are described. A description of the Data System in EBR-II is also included

  15. Sensory systems II senses other than vision

    CERN Document Server

    Wolfe, Jeremy M

    1988-01-01

    This series of books, "Readings from the Encyclopedia of Neuroscience." consists of collections of subject-clustered articles taken from the Encyclopedia of Neuroscience. The Encyclopedia of Neuroscience is a reference source and compendium of more than 700 articles written by world authorities and covering all of neuroscience. We define neuroscience broadly as including all those fields that have as a primary goal the under­ standing of how the brain and nervous system work to mediate/control behavior, including the mental behavior of humans. Those interested in specific aspects of the neurosciences, particular subject areas or specialties, can of course browse through the alphabetically arranged articles of the En­ cyclopedia or use its index to find the topics they wish to read. However. for those readers-students, specialists, or others-who will find it useful to have collections of subject-clustered articles from the Encyclopedia, we issue this series of "Readings" in paperback. Students in neuroscienc...

  16. Phenomenological studies and modelling of the gaseous impurities oxidation and adsorption mechanisms in helium: application for the purification system optimization in gas cooled nuclear reactors

    International Nuclear Information System (INIS)

    Legros, F.

    2008-01-01

    In GEN IV studies on future fission nuclear reactors, two concepts using helium as a coolant have been selected: GFR and VHTR. Among radioactive impurities and dusts, helium can contain H 2 , CO, CH 4 , CO 2 , H 2 O, O 2 , as well as nitrogenous species. To optimize the reactor functioning and lifespan, it is necessary to control the coolant chemical composition using a dedicated purification system. A pilot designed at the CEA allows studying this purification system. Its design includes three unit operations: H 2 and CO oxidation on CuO, then two adsorption steps. This study aims at providing a detailed analysis of the first and second purification steps, which have both been widely studied experimentally at laboratory scale. A first modelling based on a macroscopic approach was developed to represent the behaviour of the reactor and has shown that the CuO fixed bed conversion is dependent on the chemistry (mass transfer is not an issue) and is complete. The results of the structural analysis of the solids allow considering the CuO as particles made of 200 nm diameter grains. Hence, a new model at grain scale is proposed. It is highlighted that the kinetic constants from these two models are related with a scale factor which depends on geometry. A competition between carbon monoxide and hydrogen oxidation has been shown. Activation energies are around 30 kJ.mol-1. Simulation of the simultaneous oxidations leads to consider CO preferential adsorption. A similar methodology has been applied for CO 2 and H 2 O adsorption. The experimental isotherms showed a Langmuir type adsorption. Using this model, experimental and theoretical results agree. (author) [fr

  17. Characterization of optical systems for the ALPS II experiment

    International Nuclear Information System (INIS)

    Spector, Aaron D.; Baehre, Robin; Willke, Benno; Hannover Univ.

    2016-09-01

    ALPS II is a light shining through a wall style experiment that will use the principle of resonant enhancement to boost the conversion and reconversion probabilities of photons to relativistic WISPs. This will require the use of long baseline low-loss optical cavities. Very high power build up factors in the cavities must be achieved in order to reach the design sensitivity of ALPS II. This necessitates a number of different sophisticated optical and control systems to maintain the resonance and ensure maximal coupling between the laser and the cavity. In this paper we report on the results of the characterization of these optical systems with a 20m cavity and discuss the results in the context of ALPS II.

  18. System modeling and simulation at EBR-II

    International Nuclear Information System (INIS)

    Dean, E.M.; Lehto, W.K.; Larson, H.A.

    1986-01-01

    The codes being developed and verified using EBR-II data are the NATDEMO, DSNP and CSYRED. NATDEMO is a variation of the Westinghouse DEMO code coupled to the NATCON code previously used to simulate perturbations of reactor flow and inlet temperature and loss-of-flow transients leading to natural convection in EBR-II. CSYRED uses the Continuous System Modeling Program (CSMP) to simulate the EBR-II core, including power, temperature, control-rod movement reactivity effects and flow and is used primarily to model reactivity induced power transients. The Dynamic Simulator for Nuclear Power Plants (DSNP) allows a whole plant, thermal-hydraulic simulation using specific component and system models called from libraries. It has been used to simulate flow coastdown transients, reactivity insertion events and balance-of-plant perturbations

  19. The NASA F-15 Intelligent Flight Control Systems: Generation II

    Science.gov (United States)

    Buschbacher, Mark; Bosworth, John

    2006-01-01

    The Second Generation (Gen II) control system for the F-15 Intelligent Flight Control System (IFCS) program implements direct adaptive neural networks to demonstrate robust tolerance to faults and failures. The direct adaptive tracking controller integrates learning neural networks (NNs) with a dynamic inversion control law. The term direct adaptive is used because the error between the reference model and the aircraft response is being compensated or directly adapted to minimize error without regard to knowing the cause of the error. No parameter estimation is needed for this direct adaptive control system. In the Gen II design, the feedback errors are regulated with a proportional-plus-integral (PI) compensator. This basic compensator is augmented with an online NN that changes the system gains via an error-based adaptation law to improve aircraft performance at all times, including normal flight, system failures, mispredicted behavior, or changes in behavior resulting from damage.

  20. Production, Purification, and Identification of Cholest-4-en-3-one Produced by Cholesterol Oxidase from Rhodococcus sp. in Aqueous/Organic Biphasic System.

    Science.gov (United States)

    Wu, Ke; Li, Wei; Song, Jianrui; Li, Tao

    2015-01-01

    Cholest-4-en-3-one has positive uses against obesity, liver disease, and keratinization. It can be applied in the synthesis of steroid drugs as well. Most related studies are focused on preparation of cholest-4-en-3-one by using whole cells as catalysts, but production of high-quality cholest-4-en-3-one directly from cholesterol oxidase (COD) using an aqueous/organic two-phase system has been rarely explored. This study set up an enzymatic reaction system to produce cholest-4-en-3-one. We developed and optimized the enzymatic reaction system using COD from COX5-6 (a strain of Rhodococcus) instead of whole-cell biocatalyst. This not only simplifies and accelerates the production but also benefits the subsequent separation and purification process. Through extraction, washing, evaporation, column chromatography, and recrystallization, we got cholest-4-en-3-one with purity of 99.78%, which was identified by nuclear magnetic resonance, mass spectroscopy, and infrared spectroscopy. In addition, this optimized process of cholest-4-en-3-one production and purification can be easily scaled up for industrial production, which can largely decrease the cost and guarantee the purity of the product.

  1. Production, Purification, and Identification of Cholest-4-en-3-one Produced by Cholesterol Oxidase from sp. in Aqueous/Organic Biphasic System

    Directory of Open Access Journals (Sweden)

    Ke Wu

    2015-01-01

    Full Text Available Cholest-4-en-3-one has positive uses against obesity, liver disease, and keratinization. It can be applied in the synthesis of steroid drugs as well. Most related studies are focused on preparation of cholest-4-en-3-one by using whole cells as catalysts, but production of high-quality cholest-4-en-3-one directly from cholesterol oxidase (COD using an aqueous/organic two-phase system has been rarely explored. This study set up an enzymatic reaction system to produce cholest-4-en-3-one. We developed and optimized the enzymatic reaction system using COD from COX5-6 (a strain of Rhodococcus instead of whole-cell biocatalyst. This not only simplifies and accelerates the production but also benefits the subsequent separation and purification process. Through extraction, washing, evaporation, column chromatography, and recrystallization, we got cholest-4-en-3-one with purity of 99.78%, which was identified by nuclear magnetic resonance, mass spectroscopy, and infrared spectroscopy. In addition, this optimized process of cholest-4-en-3-one production and purification can be easily scaled up for industrial production, which can largely decrease the cost and guarantee the purity of the product.

  2. Kilowatt isotope power system phase II plan. Volume II: flight System Conceptual Design (FSCD)

    International Nuclear Information System (INIS)

    1978-03-01

    The Kilowatt Isotope Power System (KIPS) Flight System Conceptual Design (FSCD) is described. Included are a background, a description of the flight system conceptual design, configuration of components, flight system performance, Ground Demonstration System test results, and advanced development tests

  3. Open Architecture Standards and Information Systems (OASIS II ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    Open Architecture Standards and Information Systems (OASIS II) - Developing Capacity, Sharing Knowledge and Good Principles Across eHealth in Africa. Health care across much of the African continent is hampered by meager resources and a growing burden of disease, with HIV/AIDS, tuberculosis (TB) and malaria ...

  4. Multipartite electronic entanglement purification with charge detection

    Energy Technology Data Exchange (ETDEWEB)

    Sheng Yubo [Department of Physics, Tsinghua University, Beijing 100084 (China); Deng, Fu-Guo [Department of Physics, Beijing Normal University, Beijing 100875 (China); Long Guilu, E-mail: gllong@tsinghua.edu.c [Department of Physics, Tsinghua University, Beijing 100084 (China); Key Laboratory for Atomic and Molecular NanoSciences, Tsinghua University, Beijing 100084 (China); Tsinghua National Laboratory for Information Science and Technology, Beijing 100084 (China)

    2011-01-17

    We present a multipartite entanglement purification scheme in a Greenberger-Horne-Zeilinger state for electrons based on their spins and their charges. This scheme works for purification with two steps, i.e., bit-flip error correction and phase-flip error correction. By repeating these two steps, the parties in quantum communication can get some high-fidelity multipartite entangled electronic systems.

  5. Extraction and Purification of Quercitrin, Hyperoside, Rutin, and Afzelin from Zanthoxylum Bungeanum Maxim Leaves Using an Aqueous Two-Phase System.

    Science.gov (United States)

    He, Fengyuan; Li, Dengwu; Wang, Dongmei; Deng, Ming

    2016-07-01

    In this study, an aqueous two-phase system (ATPS) based on ethanol/NaH2 PO4 was developed for the extraction and purification of quercitrin, hyperoside, rutin, and afzelin from Zanthoxylum bungeanum Maxim leaves. These 4 flavonoids were 1st extracted from dried Z. bungeanum leaves using a 60% ethanol solution and subsequently added to the ATPS for further purification. The partition behavior of the 4 flavonoids in ATPS was investigated. The optimal ATPS conditions were: 29% (w/w) NaH2 PO4 , 25% (w/w) ethanol concentration, 1% (w/w) added amount of leaf extracts, no pH adjustment, and repeated 1 h extractions at 25 °C. Under the optimal conditions for the 10 g ATPS, the absolute recovery of quercitrin, hyperoside, rutin, and afzelin reached 90.3%, 83.5%, 92.3%, and 89.1%, respectively. Compared to the 60% ethanol extracts, the content of quercitrin (44.8 mg/g), hyperoside (65.6 mg/g), rutin (56.4 mg/g), and afzelin (6.84 mg/g) in the extracts increased by 49.9%, 38.8%, 45.6%, and 36.8% respectively. The extracts after ATPS also exhibited stronger antioxidant activities, the 2,2-diphenyl-1-picrylhydrazyl IC50 value (10.5 μg/mL) decreased by 41.8%, and the 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt value (966 μmol Trolox/g) and ferric reducing power value (619 μmol Trolox/g) increased by 29.8% and 53.7%, respectively. Furthermore, scale-up experiments indicated that a larger scale experiment was feasible for the purification of the 4 flavonoids. © 2016 Institute of Food Technologists®

  6. Entanglement of purification: from spin chains to holography

    Science.gov (United States)

    Nguyen, Phuc; Devakul, Trithep; Halbasch, Matthew G.; Zaletel, Michael P.; Swingle, Brian

    2018-01-01

    Purification is a powerful technique in quantum physics whereby a mixed quantum state is extended to a pure state on a larger system. This process is not unique, and in systems composed of many degrees of freedom, one natural purification is the one with minimal entanglement. Here we study the entropy of the minimally entangled purification, called the entanglement of purification, in three model systems: an Ising spin chain, conformal field theories holographically dual to Einstein gravity, and random stabilizer tensor networks. We conjecture values for the entanglement of purification in all these models, and we support our conjectures with a variety of numerical and analytical results. We find that such minimally entangled purifications have a number of applications, from enhancing entanglement-based tensor network methods for describing mixed states to elucidating novel aspects of the emergence of geometry from entanglement in the AdS/CFT correspondence.

  7. Kilowatt isotope power system. Phase II plan. Volume I. Phase II program plan

    International Nuclear Information System (INIS)

    1978-01-01

    The development of a Kilowatt Isotope Power System (KIPS) was begun in 1975 for the purpose of satisfying the power requirements of satellites in the 1980's. The KIPS is a 238 PuO 2 -fueled organic Rankine cycle turbine power system to provide a design output of 500 to 2000 W. Phase II of the overall 3-phase KIPS program is described. This volume presents a program plan for qualifying the organic Rankine power system for flight test in 1982. The program plan calls for the design and fabrication of the proposed flight power system; conducting a development and a qualification program including both environmental and endurance testing, using an electrical and a radioisotope heat source; planning for flight test and spacecraft integration; and continuing ground demonstration system testing to act as a flight system breadboard and to accumulate life data

  8. New biphasic solvent system based on cyclopentyl methyl ether for the purification of a non-polar synthetic peptide by pH-zone refining centrifugal partition chromatography.

    Science.gov (United States)

    Amarouche, Nassima; Boudesocque, Leslie; Borie, Nicolas; Giraud, Matthieu; Forni, Luciano; Butte, Alessandro; Edwards, Florence; Renault, Jean-Hugues

    2014-06-01

    A new type 1 ternary biphasic system composed of cyclopentyl methyl ether, dimethylformamide and water was developed, characterized and successfully used for the purification of a lipophilic, protected peptide by pH-zone refining centrifugal partition chromatography. The protected peptide is an 8-mer, key intermediate in bivalirudin (Angiomax®) synthesis and shows a very low solubility in the solvents usually used in liquid chromatography. All ionic groups, except the N-terminal end of the peptide, are protected by a benzyl group. The purification of this peptide was achieved with a purity of about 99.04% and a recovery of 94% using the new ternary biphasic system cyclopentyl methyl ether/dimethylformamide/water (49:40:11, v/v) in the descending pH-zone refining mode with triethylamine (28 mM) as the retainer and methanesulfonic acid (18 mM) as the eluter. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  9. RF system design for the PEP-II B Factory

    International Nuclear Information System (INIS)

    Schwarz, H.; Rimmer, R.

    1994-06-01

    The paper presents an overview of the design of the RF system for the PEP-II B Factory. An RF station consists of either two or four single-cell cavities driven by a 1.2 MW klystron through a waveguide distribution network. A variety of feedback loops stabilize the RF and its interaction with the beam. System parameters and all the relevant parameters of klystron and cavities are given

  10. PEP-II vacuum system pressure profile modeling using EXCEL

    International Nuclear Information System (INIS)

    Nordby, M.; Perkins, C.

    1994-06-01

    A generic, adaptable Microsoft EXCEL program to simulate molecular flow in beam line vacuum systems is introduced. Modeling using finite-element approximation of the governing differential equation is discussed, as well as error estimation and program capabilities. The ease of use and flexibility of the spreadsheet-based program is demonstrated. PEP-II vacuum system models are reviewed and compared with analytical models

  11. Operating experience of the EBR-II steam generating system

    International Nuclear Information System (INIS)

    Buschman, H.W.; Penney, W.H.; Quilici, M.D.; Radtke, W.H.

    1981-01-01

    The Experimental Breeder Reactor II (EBR-II) is a Liquid Metal Fast Breeder Reactor (LMFBR) with integrated power producing capability. Superheated steam is produced by eight natural circulation evaporators, two superheaters, and a conventional steam drum. Steam throttle conditions are 438 C (820 F) and 8.62 MPa (1250 psi). The designs of the evaporators and superheaters are essentially identical; both are counterflow units with low pressure nonradioactive sodium on the shell side. Safety and reliability are maximized by using duplex tubes and tubesheets. The performance of the system has been excellent and essentially trouble free. The operating experience of EBR-II provides confidence that the technology can be applied to commercial LMFBR's for an abundant supply of energy for the future. 5 refs

  12. Process simulations for the LCLS-II cryogenic systems

    Science.gov (United States)

    Ravindranath, V.; Bai, H.; Heloin, V.; Fauve, E.; Pflueckhahn, D.; Peterson, T.; Arenius, D.; Bevins, M.; Scanlon, C.; Than, R.; Hays, G.; Ross, M.

    2017-12-01

    Linac Coherent Light Source II (LCLS-II), a 4 GeV continuous-wave (CW) superconducting electron linear accelerator, is to be constructed in the existing two mile Linac facility at the SLAC National Accelerator Laboratory. The first light from the new facility is scheduled to be in 2020. The LCLS-II Linac consists of thirty-five 1.3 GHz and two 3.9 GHz superconducting cryomodules. The Linac cryomodules require cryogenic cooling for the super-conducting niobium cavities at 2.0 K, low temperature thermal intercept at 5.5-7.5 K, and a thermal shield at 35-55 K. The equivalent 4.5 K refrigeration capacity needed for the Linac operations range from a minimum of 11 kW to a maximum of 24 kW. Two cryogenic plants with 18 kW of equivalent 4.5 K refrigeration capacity will be used for supporting the Linac cryogenic cooling requirements. The cryogenic plants are based on the Jefferson Lab’s CHL-II cryogenic plant design which uses the “Floating Pressure” design to support a wide variation in the cooling load. In this paper, the cryogenic process for the integrated LCLS-II cryogenic system and the process simulation for a 4.5 K cryoplant in combination with a 2 K cold compressor box, and the Linac cryomodules are described.

  13. The NBI control system for the TJ-II

    Energy Technology Data Exchange (ETDEWEB)

    Carrasco, R. [Asociacion EURATOM-CIEMAT para Fusion, Avda. Complutense 22, 28040 Madrid (Spain)]. E-mail: ricardo.carrasco@ciemat.es; Liniers, M. [Asociacion EURATOM-CIEMAT para Fusion, Avda. Complutense 22, 28040 Madrid (Spain); Pacios, L. [Asociacion EURATOM-CIEMAT para Fusion, Avda. Complutense 22, 28040 Madrid (Spain); De la Pena, A. [Asociacion EURATOM-CIEMAT para Fusion, Avda. Complutense 22, 28040 Madrid (Spain); Lapayese, F. [Asociacion EURATOM-CIEMAT para Fusion, Avda. Complutense 22, 28040 Madrid (Spain); Wolfers, G. [Asociacion EURATOM-CIEMAT para Fusion, Avda. Complutense 22, 28040 Madrid (Spain); Alonso, J. [Asociacion EURATOM-CIEMAT para Fusion, Avda. Complutense 22, 28040 Madrid (Spain); Marcon, G. [Asociacion EURATOM-CIEMAT para Fusion, Avda. Complutense 22, 28040 Madrid (Spain); Fuentes, C. [Asociacion EURATOM-CIEMAT para Fusion, Avda. Complutense 22, 28040 Madrid (Spain)

    2006-07-15

    A description of the control system software and hardware architecture for the TJ-II Neutral Beam Injectors is given. The platform chosen is VMEbus, with controller boards running OS9 (Microware) real-time operating system. Three VME crates house several boards for performing analogue signal acquisition, signal conditioning, analogue voltage generation, digital input detection and digital output generation. A specific timing system for the injectors has been developed. At present, a user interface for monitoring and programming purposes is provided by html pages, using a web server running under the OS9 operating system. A few subsystems are now using a graphical user interface built using the Java programming language.

  14. Battle Staff Training System II: Computer-Based Instruction Supporting the Force XXI Training Program

    National Research Council Canada - National Science Library

    Wampler, Richard

    1998-01-01

    This report documents the methodology and lessons learned in the development of the Innovative Tools and Techniques for Brigade and Below Staff Training II - Battle Staff Training System II (ITTBBST-BSTS II...

  15. The NBI control system for the TJ-II

    International Nuclear Information System (INIS)

    Carrasco, R.; Liniers, M.; Pacios, L.; De la Pena, A.; Lapayese, F.; Wolfers, G.; Alonso, J.; Marcon, G.; Fuentes, C.

    2006-01-01

    A description of the control system software and hardware architecture for the TJ-II Neutral Beam Injectors is given. The platform chosen is VMEbus, with controller boards running OS9 (Microware) real-time operating system. Three VME crates house several boards for performing analogue signal acquisition, signal conditioning, analogue voltage generation, digital input detection and digital output generation. A specific timing system for the injectors has been developed. At present, a user interface for monitoring and programming purposes is provided by html pages, using a web server running under the OS9 operating system. A few subsystems are now using a graphical user interface built using the Java programming language

  16. Evaluation of a new preparative supercritical fluid chromatography system for compound library purification: the TharSFC SFC-MS Prep-100 system.

    Science.gov (United States)

    Ebinger, Katalin; Weller, Harold N; Kiplinger, Jeffrey; Lefebvre, Paul

    2011-06-01

    Preparative HPLC-MS is often the method of choice for purification of small amounts (libraries for drug discovery. The method is robust, well proven, and widely applicable. In contrast, preparative supercritical fluid chromatography coupled with mass spectrometry (SFC-MS) has seen only slow acceptance for the same application--despite some potential scientific and economic advantages. One of the reasons for slow adoption of SFC-MS is the lack of well-proven, robust, and commercially available instrumentation. In early 2009, TharSFC (a Waters Company, Pittsburgh, PA) introduced a new fully integrated system for preparative SFC-MS: The SFC-MS Prep-100. We report herein an objective evaluation of the SFC-MS Prep-100, including tests for pump and autosampler performance, sample recovery, sample carryover, fraction triggering, detector/fraction collector synchronization, and overall robustness. Our results suggest that the SFC-MS Prep-100 represents a significant advance over previous generation instrumentation. Copyright © 2011 Society for Laboratory Automation and Screening. Published by Elsevier Inc. All rights reserved.

  17. Purification and crystallization of a trimodular complex comprising the type II cohesin–dockerin interaction from the cellulosome of Clostridium thermocellum

    International Nuclear Information System (INIS)

    Adams, Jarrett J.; Pal, Gour; Yam, Katherine; Spencer, Holly L.; Jia, Zongchao; Smith, Steven P.

    2004-01-01

    A trimodular complex comprising the type II cohesin–dockerin interaction from the cellulosome of C. thermocellum has been purified and crystallized by the hanging-drop vapour-diffusion method. A native crystal and a selenomethionine derivative have been analyzed using X-ray diffraction. The high-affinity calcium-mediated type II cohesin–dockerin interaction is responsible for the attachment of the multi-enzyme cellulose-degrading complex, termed the cellulosome, to the cell surface of the thermophilic anaerobe Clostridium thermocellum. A trimodular 40 kDa complex comprising the SdbA type II cohesin and the the CipA type II dockerin–X module modular pair from the cellulosome of C. thermocellum has been crystallized. The crystals belong to space group P2 1 2 1 2 1 , with unit-cell parameters a = 45.21, b = 52.34, c = 154.69 Å. The asymmetric unit contains one molecule of the protein complex and native and selenomethionine-derivative crystals diffracted to 2.1 and 2.0 Å, respectively

  18. Neuroprotective Effects and Mechanisms of Curcumin–Cu(II and –Zn(II Complexes Systems and Their Pharmacological Implications

    Directory of Open Access Journals (Sweden)

    Fa-Shun Yan

    2017-12-01

    Full Text Available Alzheimer’s disease (AD is the main form of dementia and has a steadily increasing prevalence. As both oxidative stress and metal homeostasis are involved in the pathogenesis of AD, it would be interesting to develop a dual function agent, targeting the two factors. Curcumin, a natural compound isolated from the rhizome of Curcuma longa, is an antioxidant and can also chelate metal ions. Whether the complexes of curcumin with metal ions possess neuroprotective effects has not been evaluated. Therefore, the present study was designed to investigate the protective effects of the complexes of curcumin with Cu(II or Zn(II on hydrogen peroxide (H2O2-induced injury and the underlying molecular mechanisms. The use of rat pheochromocytoma (PC12 cells, a widely used neuronal cell model system, was adopted. It was revealed that curcumin–Cu(II complexes systems possessed enhanced O2·–-scavenging activities compared to unchelated curcumin. In comparison with unchelated curcumin, the protective effects of curcumin–Cu(II complexes systems were stronger than curcumin–Zn(II system. Curcumin–Cu(II or –Zn(II complexes systems significantly enhanced the superoxide dismutase, catalase, and glutathione peroxidase activities and attenuated the increase of malondialdehyde levels and caspase-3 and caspase-9 activities, in a dose-dependent manner. The curcumin–Cu(II complex system with a 2:1 ratio exhibited the most significant effect. Further mechanistic study demonstrated that curcumin–Cu(II or –Zn(II complexes systems inhibited cell apoptosis via downregulating the nuclear factor κB (NF-κB pathway and upregulating Bcl-2/Bax pathway. In summary, the present study found that curcumin–Cu(II or –Zn(II complexes systems, especially the former, possess significant neuroprotective effects, which indicates the potential advantage of curcumin as a promising agent against AD and deserves further study.

  19. Neuroprotective Effects and Mechanisms of Curcumin-Cu(II) and -Zn(II) Complexes Systems and Their Pharmacological Implications.

    Science.gov (United States)

    Yan, Fa-Shun; Sun, Jian-Long; Xie, Wen-Hai; Shen, Liang; Ji, Hong-Fang

    2017-12-28

    Alzheimer's disease (AD) is the main form of dementia and has a steadily increasing prevalence. As both oxidative stress and metal homeostasis are involved in the pathogenesis of AD, it would be interesting to develop a dual function agent, targeting the two factors. Curcumin, a natural compound isolated from the rhizome of Curcuma longa , is an antioxidant and can also chelate metal ions. Whether the complexes of curcumin with metal ions possess neuroprotective effects has not been evaluated. Therefore, the present study was designed to investigate the protective effects of the complexes of curcumin with Cu(II) or Zn(II) on hydrogen peroxide (H₂O₂)-induced injury and the underlying molecular mechanisms. The use of rat pheochromocytoma (PC12) cells, a widely used neuronal cell model system, was adopted. It was revealed that curcumin-Cu(II) complexes systems possessed enhanced O₂ ·- -scavenging activities compared to unchelated curcumin. In comparison with unchelated curcumin, the protective effects of curcumin-Cu(II) complexes systems were stronger than curcumin-Zn(II) system. Curcumin-Cu(II) or -Zn(II) complexes systems significantly enhanced the superoxide dismutase, catalase, and glutathione peroxidase activities and attenuated the increase of malondialdehyde levels and caspase-3 and caspase-9 activities, in a dose-dependent manner. The curcumin-Cu(II) complex system with a 2:1 ratio exhibited the most significant effect. Further mechanistic study demonstrated that curcumin-Cu(II) or -Zn(II) complexes systems inhibited cell apoptosis via downregulating the nuclear factor κB (NF-κB) pathway and upregulating Bcl-2/Bax pathway. In summary, the present study found that curcumin-Cu(II) or -Zn(II) complexes systems, especially the former, possess significant neuroprotective effects, which indicates the potential advantage of curcumin as a promising agent against AD and deserves further study.

  20. SCORPION II persistent surveillance system with universal gateway

    Science.gov (United States)

    Coster, Michael; Chambers, Jonathan; Brunck, Albert

    2009-05-01

    This paper addresses improvements and benefits derived from the next generation Northrop Grumman SCORPION II family of persistent surveillance and target recognition systems produced by the Xetron campus in Cincinnati, Ohio. SCORPION II reduces the size, weight, and cost of all SCORPION components in a flexible, field programmable system that is easier to conceal, backward compatible, and enables integration of over forty Unattended Ground Sensor (UGS) and camera types from a variety of manufacturers, with a modular approach to supporting multiple Line of Sight (LOS) and Beyond Line of Sight (BLOS) communications interfaces. Since 1998 Northrop Grumman has been integrating best in class sensors with its proven universal modular Gateway to provide encrypted data exfiltration to Common Operational Picture (COP) systems and remote sensor command and control. In addition to being fed to COP systems, SCORPION and SCORPION II data can be directly processed using a common sensor status graphical user interface (GUI) that allows for viewing and analysis of images and sensor data from up to seven hundred SCORPION system Gateways on single or multiple displays. This GUI enables a large amount of sensor data and imagery to be used for actionable intelligence as well as remote sensor command and control by a minimum number of analysts.

  1. Automated System for Control of the Vacuum Diagnostic System for the TJ-II

    International Nuclear Information System (INIS)

    Lopez Sanchez, A.; Montoro Peinado, A.; Encabo Fernandez, J.; Gama de la Serrano, J.; Sanchez Sarabia, E.

    1999-12-01

    This report describes the monitoring and remote control systems belonging to the high vacuum systems of the TJ-II diagnostics. These systems are part of each diagnostic and their control has been integrated into the automata that carries out this task. All the controllers are connected through a Profibus network, so as to interchange data between themselves as well as between the general system of TJ-II. (Author)

  2. Biosphere II: engineering of manned, closed ecological systems.

    Science.gov (United States)

    Dempster, W F

    1991-01-01

    Space Biospheres and Ventures, a private, for-profit firm, has undertaken a major research and development project in the study of biospheres, with the objective of creating and producing biospheres. Biosphere II-scheduled for completion in March 1991-will be essentially isolated from the existing biosphere by a closed structure, composed of components derived from the existing biosphere. Like the biosphere of the Earth, Biosphere II will be essentially closed to exchanges of material or living organisms with the surrounding environment and open to energy and information exchanges. Also, like the biosphere of the Earth, Biosphere II will contain five kingdoms of life, a variety of ecosystems, plus humankind, culture, and technics. The system is designed to be complex, stable and evolving throughout its intended 100-year lifespan, rather than static. Biosphere II will cover approximately 1.3 hectare and contain 200,000 m3 in volume, with seven major biomes: tropical rainforest, tropical savannah, marsh, marine, desert, intensive agriculture, and human habitat. An interdisciplinary team of leading scientific, ecological, management, architectural, and engineering consultants have been contracted by Space Biospheres Ventures for the project. Potential applications for biospheric systems include scientific and ecological management research, refuges for endangered species, and life habitats for manned stations on spacecraft or other planets.

  3. The simulation library of the Belle II software system

    Science.gov (United States)

    Kim, D. Y.; Ritter, M.; Bilka, T.; Bobrov, A.; Casarosa, G.; Chilikin, K.; Ferber, T.; Godang, R.; Jaegle, I.; Kandra, J.; Kodys, P.; Kuhr, T.; Kvasnicka, P.; Nakayama, H.; Piilonen, L.; Pulvermacher, C.; Santelj, L.; Schwenker, B.; Sibidanov, A.; Soloviev, Y.; Starič, M.; Uglov, T.

    2017-10-01

    SuperKEKB, the next generation B factory, has been constructed in Japan as an upgrade of KEKB. This brand new e+ e- collider is expected to deliver a very large data set for the Belle II experiment, which will be 50 times larger than the previous Belle sample. Both the triggered physics event rate and the background event rate will be increased by at least 10 times than the previous ones, and will create a challenging data taking environment for the Belle II detector. The software system of the Belle II experiment is designed to execute this ambitious plan. A full detector simulation library, which is a part of the Belle II software system, is created based on Geant4 and has been tested thoroughly. Recently the library has been upgraded with Geant4 version 10.1. The library is behaving as expected and it is utilized actively in producing Monte Carlo data sets for various studies. In this paper, we will explain the structure of the simulation library and the various interfaces to other packages including geometry and beam background simulation.

  4. The TJ-II data acquisition system: an overview

    International Nuclear Information System (INIS)

    Vega, J.; Cremy, C.; Sanchez, E.; Portas, A.

    1999-01-01

    The data acquisition system for the TJ-II fusion machine has been developed to coordinate actions among the several experimental systems devoted to data capture and storage: instrumentation mainframes (VXI, VME, CAMAC), control systems of diagnostics and a host-centralized database. Connectivity between these elements is achieved through local area networks, which ensure both good connections and system growth capability. Three hundred VXI based digitizer channels have been developed for TJ-II diagnostics. They are completely software programmable and provide signal analog conditioning. In addition, some of them supply a programmable DSP for real time signal processing. Data will be stored in a central server using a special compression technique that allows compaction rates of over 80%. A specific application software has been developed to provide user interface for digitizer programming, signal visualization and data processing during TJ-II discharges. The software is an event based application that can be remotely launched from any X terminal An authentication mechanism restricts access to authorised users only. (orig.)

  5. From microsystems technology to the Saenger II space transportation system

    Science.gov (United States)

    Vogels, Hanns Arnt

    The role of space projects as drivers and catalysts of technology advances is discussed and illustrated from the perspective of the West German aerospace industry, summarizing a talk presented at the 1986 meeting of the German aerospace society DGLR. The history of space-transportation-system (STS) technology since the 1950s is traced, emphasizing the needs for greater payload weights and lower costs, and the design concept of Saenger II, a proposed two-stage ESA STS employing a hypersonic jet transport aircraft as its first stage, is outlined. It is argued that experience gained in developing the rocket-launched Hermes STS will be applicable to the second stage of Saenger II. Recent developments in microsystems (combining microelectronics, micromechanics, and microoptics), advanced materials (fiber-reinforced plastics, metals, and ceramics), and energy technology (hydrogen-based systems and solar cells) are surveyed, and their applicability to STSs is considered.

  6. Efficiencies of different flue gas purification systems in waste incinerators. Experimental report; Untersuchungen zur Effizienz von unterschiedlichen Systemen zur Rauchgasreinigung bei Muellverbrennungsanlagen. Untersuchungsbericht

    Energy Technology Data Exchange (ETDEWEB)

    Berghoff, R. (comp.)

    2001-07-01

    This investigation was carried out on behalf of the Environmental Office of the state of Nordrhein-Westfalen. Apart from the current and projected exhaust purification systems, also three exemplary systems were analyzed from the ecological and economic point of view. Effects on pollutant freights were established in consideration of energy consumption and the specific cost of waste treatment for each technology. [German] Das konkrete Ziel dieser vom Landesumweltamt NRW vergebenen Untersuchung war es, neben einer Uebersicht ueber die zzt. eingesetzten und auch geplanten Abgasreinigungssysteme (RGR) bei Muellverbrennungsanlagen, die Vor- und die Nachteile von 3 beispielhaft ausgewaehlten Systemen in einem oekologischen und oekonomischen Vergleich knapp und begrenzt darstellen zu lassen. Als Ergebnis wurden die Auswirkungen der unterschiedlichen Verfahren auf die Frachten der emittierten Schadstoffe unter Beruecksichtigung der unterschiedlichen Energieverbraeuche ermittelt und dargestellt. Darueber hinaus wurden die spezifischen Muellbehandlungskosten bei den jeweiligen Gasreinigungstechniken errechnet. (orig.)

  7. Absorption heat pump integrated in an effluent purification system; Bomba de calor por absorcion integrada a un sistema de purificacion de efluentes

    Energy Technology Data Exchange (ETDEWEB)

    Santoyo, Socrates; Siqueiros, Javier; Heard, Christopher; Santoyo, Edgar [Instituto de Investigaciones Electricas, Cuernavaca (Mexico)

    1996-12-31

    The results derived of the integration of an absorption heat pump to an industrial effluents purification system, are presented. The advantages of these heat pumps with respect to heat pumps by mechanical compression of vapor, as well as the advantages in using absorption heat pumps in simple distillation systems, are mentioned. Finally, a description is made of the equipment designed and built, as well as the results obtained in a preliminary test. [Espanol] Se presentan los resultados derivados de la integracion de una bomba de calor por absorcion a un sistema de purificacion de efluentes industriales. Se mencionan las ventajas de este tipo de bombas de calor con respecto a las de calor por compresion mecanica de vapor, asi como las ventajas de usar bombas de calor en sistemas de destilacion simple. Finalmente, se describe el equipo disenado y construido, asi como los resultados obtenidos de una prueba preliminar.

  8. Absorption heat pump integrated in an effluent purification system; Bomba de calor por absorcion integrada a un sistema de purificacion de efluentes

    Energy Technology Data Exchange (ETDEWEB)

    Santoyo, Socrates; Siqueiros, Javier; Heard, Christopher; Santoyo, Edgar [Instituto de Investigaciones Electricas, Cuernavaca (Mexico)

    1997-12-31

    The results derived of the integration of an absorption heat pump to an industrial effluents purification system, are presented. The advantages of these heat pumps with respect to heat pumps by mechanical compression of vapor, as well as the advantages in using absorption heat pumps in simple distillation systems, are mentioned. Finally, a description is made of the equipment designed and built, as well as the results obtained in a preliminary test. [Espanol] Se presentan los resultados derivados de la integracion de una bomba de calor por absorcion a un sistema de purificacion de efluentes industriales. Se mencionan las ventajas de este tipo de bombas de calor con respecto a las de calor por compresion mecanica de vapor, asi como las ventajas de usar bombas de calor en sistemas de destilacion simple. Finalmente, se describe el equipo disenado y construido, asi como los resultados obtenidos de una prueba preliminar.

  9. LCLS-II high power RF system overview and progress

    Energy Technology Data Exchange (ETDEWEB)

    Yeremian, Anahid Dian

    2015-10-07

    A second X-ray free electron laser facility, LCLS-II, will be constructed at SLAC. LCLS-II is based on a 1.3 GHz, 4 GeV, continuous-wave (CW) superconducting linear accelerator, to be installed in the first kilometer of the SLAC tunnel. Multiple types of high power RF (HPRF) sources will be used to power different systems on LCLS-II. The main 1.3 GHz linac will be powered by 280 1.3 GHz, 3.8 kW solid state amplifier (SSA) sources. The normal conducting buncher in the injector will use four more SSAs identical to the linac SSAs but run at 2 kW. Two 185.7 MHz, 60 kW sources will power the photocathode dual-feed RF gun. A third harmonic linac section, included for linearizing the bunch energy spread before the first bunch compressor, will require sixteen 3.9 GHz sources at about 1 kW CW. A description and an update on all the HPRF sources of LCLS-II and their implementation is the subject of this paper.

  10. Laboratory of minerals purification

    International Nuclear Information System (INIS)

    2002-01-01

    The laboratory of minerals purification was organized in 1962 where with application of modern physical and chemical methods were investigated the mechanism of flotation reagents interaction with minerals' surface, was elaborated technologies on rising complexity of using of republic's minerals

  11. Fiscal 1975 Sunshine Project research report. Research on hydrogen purification storage transport systems, and their safety technologies; 1975 nendo suiso no seisei, yuso, chozo system oyobi hoan gijutsu ni kansuru kenkyu seika hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1976-05-31

    In fiscal 1975, research was made on (1) some problems and their solutions for practical hydrogen purification storage transport (HPST) systems, and (2) analysis and optimization of HPST systems. The material-energy flow diagram and rough hydrogen system relevance tree were prepared, and the position of HPST system in hydrogen systems was considered together with use of liquid hydrogen. In (1), the current technologies and problems were arranged for every component of purification system, storage system, transport and distribution system, and metal hydride. Study was also made on some problems and solutions for their R and D. In (2), study was made on the basic model of HPST, and the compatibility with a total energy system. Overseas and Industrial Research Institute (IRI)'s trial calculation results were arranged for HPST cost data as input data for trial calculation of optimization. As for a safety and reliability, study was made on the target, system and main R and D fields for the safety management of HPST system, referring to the concept of safety managements in nuclear power generation and LNG system. (NEDO)

  12. Physics Detector Simulation Facility Phase II system software description

    International Nuclear Information System (INIS)

    Scipioni, B.; Allen, J.; Chang, C.; Huang, J.; Liu, J.; Mestad, S.; Pan, J.; Marquez, M.; Estep, P.

    1993-05-01

    This paper presents the Physics Detector Simulation Facility (PDSF) Phase II system software. A key element in the design of a distributed computing environment for the PDSF has been the separation and distribution of the major functions. The facility has been designed to support batch and interactive processing, and to incorporate the file and tape storage systems. By distributing these functions, it is often possible to provide higher throughput and resource availability. Similarly, the design is intended to exploit event-level parallelism in an open distributed environment

  13. Process development, design and operation of off-line purification system for oil-contaminated impure heavy water

    International Nuclear Information System (INIS)

    Bose, H.; Rakesh Kumar; Gandhi, H.C.; Unny, V.K.P.; Ghosh, S.K.; Mishra, Vivek; Shukla, D.K.; Duraisamy, S.; Agarwal, S.K.

    2004-01-01

    A large volume of degraded, tritiated heavy water contaminated with mineral oil and ionic impurities have accumulated at Dhruva in the past years of reactor operation as a result of routine operation and maintenance activities. The need was felt for a simple and efficient process that could be set up and operated locally at site using readily available materials, to purify the accumulated impure heavy waters at Dhruva so as to make them acceptable at the up gradation facilities. After a detailed laboratory study, a three stage clean-up process was developed which could purify a highly turbid oil-water emulsion to yield clear, oil-free and de-mineralized heavy water at reasonable rates of volume through-put. Based on the laboratory data, a suitably scaled up purification unit has been designed and commissioned which in the past few months has processed a sizeable volume of oil-contaminated heavy water waste from Dhruva, with most satisfactory results

  14. Liquid metal purification device

    International Nuclear Information System (INIS)

    Sakai, Takao; Shimoyashiki, Shigehiro.

    1992-01-01

    The device of the present invention concerns a liquid metal purification device for removing and purifying impuries in liquid metal sodium used as coolants of an FBR type reactor. A vessel having a group of pipes made of hydrogen permeable metal at the inside thereof is disposed to the inlet pipeline of a cold trap. The group of hydrogen permeable metal pipes is connected to an exhaust pipe and a vacuum pump, so that the inside of the pipes is exhausted. Liquid metal sodium branched from the main pipeline of a coolant system passes through the outer side of the group of the hydrogen permeable metal pipes. In this cae, hydrogen contained as impurities in the liquid metal sodium diffuses and permeates the hydrogen permeation metal pipes and enters into the pipe group and is discharged out of the system by the vacuum pump. This can mitigate the hydrogen removing burden of the cold trap, to extend the device life time. (I.N.)

  15. Design layout for gas monitoring system II, GMS-2, computer system

    International Nuclear Information System (INIS)

    Vo, V.; Philipp, B.L.; Manke, M.P.

    1995-01-01

    This document provides a general overview of the computer systems software that perform the data acquisition and control for the 241-SY-101 Gas Monitoring System II (GMS-2). It outlines the system layout, and contains descriptions of components and the functions they perform. The GMS-2 system was designed and implemented by Los Alamos National Laboratory and supplied to Westinghouse Hanford Company

  16. Implant Angle Monitor System of MC3-II

    International Nuclear Information System (INIS)

    Sato, Fumiaki; Sano, Makoto; Nakaoka, Hiroaki; Fujii, Yoshito; Kudo, Tetuya; Nakanishi, Makoto; Koike, Masazumi; Fujino, Yasushi

    2008-01-01

    Precise implant angle control is required for the latest generation of ion implanters to meet further shrink semiconductor device requirements. Especially, the highest angle accuracy is required for Halo implant process of Logic devices. The Halo implant angle affects the device performance, because slight differences of beam divergence change the overlap profile towards the extension. Additionally, twist angle accuracy is demanded in case of channeling angle implant. Therefore monitoring beam angles and wafer twist angles is important. A new monitoring system for the MC3-II, SEN Corp.'s single wafer type medium current implanter has been developed. This paper describes the angle control performance and monitoring system of the MC3-II. For the twist angle control, we developed a wafer notch angle monitor. The system monitors the wafer notch image on the platen. And the notch angle variation is calculated by using image processing method. It is also able to adjust the notch angle according to the angle error. For the tilt angle control, we developed a vertical beam profile monitor. The monitor system can detect beam profile of vertical directions with horizontally scanning beam. It also measures beam angles of a tilt direction to a wafer. The system configuration and sample beam data are presented.

  17. Urban Integrated Industrial Cogeneration Systems Analysis. Phase II final report

    Energy Technology Data Exchange (ETDEWEB)

    1984-01-01

    Through the Urban Integrated Industrial Cogeneration Systems Analysis (UIICSA), the City of Chicago embarked upon an ambitious effort to identify the measure the overall industrial cogeneration market in the city and to evaluate in detail the most promising market opportunities. This report discusses the background of the work completed during Phase II of the UIICSA and presents the results of economic feasibility studies conducted for three potential cogeneration sites in Chicago. Phase II focused on the feasibility of cogeneration at the three most promising sites: the Stockyards and Calumet industrial areas, and the Ford City commercial/industrial complex. Each feasibility case study considered the energy load requirements of the existing facilities at the site and the potential for attracting and serving new growth in the area. Alternative fuels and technologies, and ownership and financing options were also incorporated into the case studies. Finally, site specific considerations such as development incentives, zoning and building code restrictions and environmental requirements were investigated.

  18. Chromatographic studies on thermal and radiolytic degradation of n-dodecane-HNO3 two components system and its purification employing silica gel treatment

    International Nuclear Information System (INIS)

    Kadam, Prashant; Kaushik, C.P.; Ozarde, P.D.; Bindu, M.; Tripathi, S.C.; Jambunathan, U.; Pandit, G.G.

    2005-01-01

    This paper describes the studies carried out to examine the extent of degradation of n-dodecane at elevated temperature for different time periods and the effect of gamma radiolysis at different absorbed dose, in n-dodecane- nitric acid two components system. The studies also involved the identification of the degradation products formed during above process using GC-MS. A large number of degradation products were observed. Formation of degradation products increases with absorbed dose and time of heating. Further purification of degraded dodecane was carried out with silica gel crystals as an adsorbent. Treated dodecane samples were subjected to GC/GC-MS analysis, to confirm the extent of removal degradation products. (author)

  19. NUKAB system use with the PICKER DYNA CAMERA II

    International Nuclear Information System (INIS)

    Collet, H.; Faurous, P.; Lehn, A.; Suquet, P.

    Present-day data processing units connected to scintillation gamma cameras can make use of cabled programme or recorded programme systems. The NUKAB system calls on the latter technique. The central element of the data processing unit, connected to the PICKER DYNA CAMERA II output, consists of a DIGITAL PDP 8E computer with 12-bit technological words. The use of a 12-bit technological format restricts the possibilities of digitalisation, 64x64 images representing the practical limit. However the NUKAB system appears well suited to the processing of data from gamma cameras at present in service. The addition of output terminals of the tracing panel type should widen the possibilities of the system. It seems that the 64x64 format is not a handicap in view of the resolution power of the detectors [fr

  20. In vivo phosphorylation of a peptide tag for protein purification.

    Science.gov (United States)

    Goux, Marine; Fateh, Amina; Defontaine, Alain; Cinier, Mathieu; Tellier, Charles

    2016-05-01

    To design a new system for the in vivo phosphorylation of proteins in Escherichia coli using the co-expression of the α-subunit of casein kinase II (CKIIα) and a target protein, (Nanofitin) fused with a phosphorylatable tag. The level of the co-expressed CKIIα was controlled by the arabinose promoter and optimal phosphorylation was obtained with 2 % (w/v) arabinose as inductor. The effectiveness of the phosphorylation system was demonstrated by electrophoretic mobility shift assay (NUT-PAGE) and staining with a specific phosphoprotein-staining gel. The resulting phosphorylated tag was also used to purify the phosphoprotein by immobilized metal affinity chromatography, which relies on the specific interaction of phosphate moieties with Fe(III). The use of a single tag for both the purification and protein array anchoring provides a simple and straightforward system for protein analysis.

  1. Phase II Vault Testing of the Argonne RFID System

    International Nuclear Information System (INIS)

    Willoner, T.; Turlington, R.; Koenig, R.

    2012-01-01

    The U.S. Department of Energy (DOE) (Environmental Management (EM), Office of Packaging and Transportation (EM-45)) Packaging and Certification Program (DOE PCP) has developed a Radio Frequency Identification (RFID) tracking and monitoring system, called ARG-US, for the management of nuclear materials packages during transportation and storage. The performance of the ARG-US RFID equipment and system has been fully tested in two demonstration projects in April 2008 and August 2009. With the strong support of DOE-SR and DOE PCP, a field testing program was completed in Savannah River Site's K-Area Material Storage (KAMS) Facility, an active Category I Plutonium Storage Facility, in 2010. As the next step (Phase II) of continued vault testing for the ARG-US system, the Savannah River Site K Area Material Storage facility has placed the ARG-US RFIDs into the 910B storage vault for operational testing. This latest version (Mark III) of the Argonne RFID system now has the capability to measure radiation dose and dose rate. This paper will report field testing progress of the ARG-US RFID equipment in KAMS, the operability and reliability trend results associated with the applications of the system, and discuss the potential benefits in enhancing safety, security and materials accountability. The purpose of this Phase II K Area test is to verify the accuracy of the radiation monitoring and proper functionality of the ARG-US RFID equipment and system under a realistic environment in the KAMS facility. Deploying the ARG-US RFID system leads to a reduced need for manned surveillance and increased inventory periods by providing real-time access to status and event history traceability, including environmental condition monitoring and radiation monitoring. The successful completion of the testing program will provide field data to support a future development and testing. This will increase Operation efficiency and cost effectiveness for vault operation. As the next step (Phase

  2. PHASE II VAULT TESTING OF THE ARGONNE RFID SYSTEM

    Energy Technology Data Exchange (ETDEWEB)

    Willoner, T.; Turlington, R.; Koenig, R.

    2012-06-25

    The U.S. Department of Energy (DOE) (Environmental Management [EM], Office of Packaging and Transportation [EM-45]) Packaging and Certification Program (DOE PCP) has developed a Radio Frequency Identification (RFID) tracking and monitoring system, called ARG-US, for the management of nuclear materials packages during transportation and storage. The performance of the ARG-US RFID equipment and system has been fully tested in two demonstration projects in April 2008 and August 2009. With the strong support of DOE-SR and DOE PCP, a field testing program was completed in Savannah River Site's K-Area Material Storage (KAMS) Facility, an active Category I Plutonium Storage Facility, in 2010. As the next step (Phase II) of continued vault testing for the ARG-US system, the Savannah River Site K Area Material Storage facility has placed the ARG-US RFIDs into the 910B storage vault for operational testing. This latest version (Mark III) of the Argonne RFID system now has the capability to measure radiation dose and dose rate. This paper will report field testing progress of the ARG-US RFID equipment in KAMS, the operability and reliability trend results associated with the applications of the system, and discuss the potential benefits in enhancing safety, security and materials accountability. The purpose of this Phase II K Area test is to verify the accuracy of the radiation monitoring and proper functionality of the ARG-US RFID equipment and system under a realistic environment in the KAMS facility. Deploying the ARG-US RFID system leads to a reduced need for manned surveillance and increased inventory periods by providing real-time access to status and event history traceability, including environmental condition monitoring and radiation monitoring. The successful completion of the testing program will provide field data to support a future development and testing. This will increase Operation efficiency and cost effectiveness for vault operation. As the next step

  3. EBR-II Cover Gas Cleanup System upgrade process control system structure

    International Nuclear Information System (INIS)

    Carlson, R.B.; Staffon, J.D.

    1992-01-01

    The Experimental Breeder Reactor II (EBR-II) Cover Gas Cleanup System (CGCS) control system was upgraded in 1991 to improve control and provide a graphical operator interface. The upgrade consisted of a main control computer, a distributed control computer, a front end input/output computer, a main graphics interface terminal, and a remote graphics interface terminal. This paper briefly describes the Cover Gas Cleanup System and the overall control system; describes the main control computer hardware and system software features in more detail; and, then, describes the real-time control tasks, and how they interact with each other, and how they interact with the operator interface task

  4. EBR-II Cover Gas Cleanup System upgrade distributed control and front end computer systems

    International Nuclear Information System (INIS)

    Carlson, R.B.

    1992-01-01

    The Experimental Breeder Reactor II (EBR-II) Cover Gas Cleanup System (CGCS) control system was upgraded in 1991 to improve control and provide a graphical operator interface. The upgrade consisted of a main control computer, a distributed control computer, a front end input/output computer, a main graphics interface terminal, and a remote graphics interface terminal. This paper briefly describes the Cover Gas Cleanup System and the overall control system; gives reasons behind the computer system structure; and then gives a detailed description of the distributed control computer, the front end computer, and how these computers interact with the main control computer. The descriptions cover both hardware and software

  5. Earth System Grid II, Turning Climate Datasets into Community Resources

    Energy Technology Data Exchange (ETDEWEB)

    Middleton, Don

    2006-08-01

    The Earth System Grid (ESG) II project, funded by the Department of Energy’s Scientific Discovery through Advanced Computing program, has transformed climate data into community resources. ESG II has accomplished this goal by creating a virtual collaborative environment that links climate centers and users around the world to models and data via a computing Grid, which is based on the Department of Energy’s supercomputing resources and the Internet. Our project’s success stems from partnerships between climate researchers and computer scientists to advance basic and applied research in the terrestrial, atmospheric, and oceanic sciences. By interfacing with other climate science projects, we have learned that commonly used methods to manage and remotely distribute data among related groups lack infrastructure and under-utilize existing technologies. Knowledge and expertise gained from ESG II have helped the climate community plan strategies to manage a rapidly growing data environment more effectively. Moreover, approaches and technologies developed under the ESG project have impacted datasimulation integration in other disciplines, such as astrophysics, molecular biology and materials science.

  6. WARRIOR II, a high performance modular electric robot system

    International Nuclear Information System (INIS)

    Downton, G.C.

    1996-01-01

    A high performance electric robot, WARRIOR, was built for in-reactor welding at the Oldbury nuclear power plant in the United Kingdom in the mid 1980s. WARRIOR II has been developed as a lighter, smaller diameter articulated welding robot which can be deployed on its umbilical down a stand pipe for remote docking with the manipulator system which delivers it to its work site. A key feature of WARRIOR II has been the development of a prototype spherical modular joint. The module provides the drive torque necessary to motivate the robot arm, acts as the joint bearing, has standard mechanical interfaces for the limb sections, accurately measures the joint angle and has cable services running through the centre. It can act either as a bend or rotate joint and the interconnecting limb sections need only to be simple tubular sections. A wide range of manipulator configurations to suit the access constraints of particular problems can be achieved with a set of joint modules and limb sections. A general purpose motion controller has also been developed which is capable of kinematically controlling any configuration of WARRIOR II thus contributing to the realisation of the concept of a general purpose tool which can be used over and over again, at short notice, in any situation where a high precision, light weight, versatile manipulator is required. (UK)

  7. Improving the technology of purification of gas emissions petrochemical industries

    OpenAIRE

    USMANOVA R.R.; ZAIKOV G.E.

    2014-01-01

    The technology of cleaning of gas emissions flares in the production of synthetic rubber. Developed dynamic scrubber for scrubbing gas emissions. Complex studies served as the basis for the design of an air purification system of industrial premises. Purification of gas emissions before combustion in flares has significantly reduced air pollution by toxic substances.

  8. One-step purification of E. coli elongation factor Tu

    DEFF Research Database (Denmark)

    Knudsen, Charlotte Rohde; Clark, Brian F. C.; Degn, B

    1993-01-01

    The tuf A gene, encoding the E. coli elongation factor Tu, was cloned in the pGEX gene fusion system. Upon expression EF-Tu is fused to glutathione-S-transferase serving as a purification handle with affinity for glutathione immobilised on agarose. This allows purification of EF-Tu in a one...

  9. AgIIS, Agricultural Irrigation Imaging System, design and application

    Science.gov (United States)

    Haberland, Julio Andres

    Remote sensing is a tool that is increasingly used in agriculture for crop management purposes. A ground-based remote sensing data acquisition system was designed, constructed, and implemented to collect high spatial and temporal resolution data in irrigated agriculture. The system was composed of a rail that mounts on a linear move irrigation machine, and a small cart that runs back and forth on the rail. The cart was equipped with a sensors package that measured reflectance in four discrete wavelengths (550 nm, 660 nm, 720 nm, and 810 nm, all 10 nm bandwidth) and an infrared thermometer. A global positioning system and triggers on the rail indicated cart position. The data was postprocessed in order to generate vegetation maps, N and water status maps and other indices relevant for site-specific crop management. A geographic information system (GIS) was used to generate images of the field on any desired day. The system was named AgIIS (A&barbelow;gricultural I&barbelow;rrigation I&barbelow;maging S&barbelow;ystem). This ground based remote sensing acquisition system was developed at the Agricultural and Biosystems Engineering Department at the University of Arizona in conjunction with the U.S. Water Conservation Laboratory in Phoenix, as part of a cooperative study primarily funded by the Idaho National Environmental and Engineering Laboratory. A second phase of the study utilized data acquired with AgIIS during the 1999 cotton growing season to model petiole nitrate (PNO3 -) and total leaf N. A latin square experimental design with optimal and low water and optimal and low N was used to evaluate N status under water and no water stress conditions. Multivariable models were generated with neural networks (NN) and multilinear regression (MLR). Single variable models were generated from chlorophyll meter readings (SPAD) and from the Canopy Chlorophyll Content Index (CCCI). All models were evaluated against observed PNO3- and total leaf N levels. The NN models

  10. Cost effective purification of intein based syntetic cationic antimicrobial peptide expressed in cold shock expression system using salt inducible E. coli GJ1158

    Directory of Open Access Journals (Sweden)

    Seetha Ram Kotra

    2014-03-01

    Full Text Available Objective:Synthetic cationic antimicrobial peptide (SC-AMP is an important and upcoming therapeutic molecule against onventional antibiotics. In this study, an attempt was made to purify the SC-AMP without the enzymatic cleavage of the affinity tag, by using an intein-based system. Methods:The intein sequence was amplified from pTYB11 vector using PCR methodologies and the N-terminal of intein was ligated with SC-AMP. The designed construct, intein-SC-AMP was cloned into MCS region of cold shock expression vector, pCOLDI and the recombinant peptide was purified on a chitin affinity column by cleaving intein with 50 mM DTT without applying enzymatic cleavage. Later the peptide was quantified and its antibacterial activity of the purified peptide was studied using well diffusion method. Results: Initially, intein-SC-AMP was expressed as a fusion protein in both IPTG inducible E. coli BL21(DE3 and salt inducible E. coli GJ1158. Single step purification using CBD (chitin binding domain - intein tag in salt inducible E. coli GJ1158, yields the SC-AMP in the soluble form at a oncentration of 208 mg/L. The antibacterial activity and minimal inhibitory concentration (MIC of the purified SC-AMP was studied against both Gram positive and Gram negative microorganisms. Conclusion: For the first time, single step purification of soluble SC-AMP was carried out using chitin-binding domain affinity tag in salt inducible E. coli GJ1158 without an application of enzymatic cleavage. J Microbiol Infect Dis 2014;4(1:13-19

  11. Integrating a post-column makeup pump into preparative supercritical fluid chromatography systems to address stability and recovery issues during purifications.

    Science.gov (United States)

    Bajpai, Lakshmikant; Naidu, Harshavardhan; Asokan, Kathiravan; Shaik, Khaja Mohiddin; Kaspady, Mahammed; Arunachalam, Piramanayagam; Wu, Dauh-Rurng; Mathur, Arvind; Sarabu, Ramakanth

    2017-08-18

    Purification of many pharmaceutical compounds by supercritical fluid chromatography (SFC) has always been challenging because of degradation of compound during the isolation step in the presence of acidic or basic modifiers in the mobile phase. Stability of such acid or base-sensitive compounds could be improved by post-column addition of a solvent containing base or acid modifier as counter ion through a make-up pump respectively to neutralize the compound fraction without affecting the resolution. One such case study has been presented in this work where the stability of a base-sensitive compound was addressed by the addition of acidic co-solvent through the make-up pump. Details of this setup and the investigation of degradation of the in-house base-sensitive compound are discussed in this paper. In addition, poor retentivity and low recovery of many non-polar compounds in SFC eluting under low co-solvent percentage is another major concern. Even though the desired separation could be achieved with low percentage of co-solvent, it's difficult to get the proper recovery after purification due to precipitation of the sample and significant aerosol formation inside the cyclone. We have demonstrated the first-time use of a post-column make-up pump on SFC 350 system to introduce additional solvent prior to cyclone to avoid the precipitation, reduce the aerosol formation and thus improve the recovery of non-polar compounds eluting under less than 10% of co-solvent. Copyright © 2017 Elsevier B.V. All rights reserved.

  12. Study of new rubber to steel adhesive systems based on Co(II and Cu(II sulphides coats

    Directory of Open Access Journals (Sweden)

    Labaj Ivan

    2018-01-01

    Full Text Available The presented paper deals with the preparation of new rubber to steel adhesive systems using the steel surface treatment with deposition of adhesive coats based on Co(II and Cu(II sulphides. Efficiency of new prepared adhesive systems containing Co(II and Cu(II sulphides has been compared with the efficiency of double layer adhesive system commonly used in industry. The chemical composition of prepared adhesive systems was determined using the EDX analysis. Scanning Electron Microscopy (SEM was used for study of topography and microstructure of prepared rubber to steel adhesive systems (Co(II, Cu(II sulphide, double layer adhesive system. For determination of adhesion strength between rubber blends and metal pieces with various adhesive systems deposited on these pieces, the test according to ASTM D429 standard relating to Rubber to metal adhesion, method A was used. For all test samples, the same type of rubber blend and the same curing conditions have been used.

  13. Photocatalytic materials and technologies for air purification.

    Science.gov (United States)

    Ren, Hangjuan; Koshy, Pramod; Chen, Wen-Fan; Qi, Shaohua; Sorrell, Charles Christopher

    2017-03-05

    Since there is increasing concern for the impact of air quality on human health, the present work surveys the materials and technologies for air purification using photocatalytic materials. The coverage includes (1) current photocatalytic materials for the decomposition of chemical contaminants and disinfection of pathogens present in air and (2) photocatalytic air purification systems that are used currently and under development. The present work focuses on five main themes. First, the mechanisms of photodegradation and photodisinfection are explained. Second, system designs for photocatalytic air purification are surveyed. Third, the photocatalytic materials used for air purification and their characteristics are considered, including both conventional and more recently developed photocatalysts. Fourth, the methods used to fabricate these materials are discussed. Fifth, the most significant coverage is devoted to materials design strategies aimed at improving the performance of photocatalysts for air purification. The review concludes with a brief consideration of promising future directions for materials research in photocatalysis. Copyright © 2016 Elsevier B.V. All rights reserved.

  14. The Argus(®) II Retinal Prosthesis System.

    Science.gov (United States)

    Luo, Yvonne Hsu-Lin; da Cruz, Lyndon

    2016-01-01

    The Argus(®) II Retinal Prosthesis System (Second Sight Medical Products) is the first prosthetic vision device to obtain regulatory approval in both Europe and the USA. As such it has entered the commercial market as a treatment for patients with profound vision loss from end-stage outer retinal disease, predominantly retinitis pigmentosa. To date, over 100 devices have been implanted worldwide, representing the largest group of patients currently treated with visual prostheses. The system works by direct stimulation of the relatively preserved inner retina via epiretinal microelectrodes, thereby replacing the function of the degenerated photoreceptors. Visual information from a glasses-mounted video camera is converted to a pixelated image by an external processor, before being transmitted to the microelectrode array at the macula. Elicited retinal responses are then relayed via the normal optic nerve to the cortex for interpretation. We reviewed the animal and human studies that led to the development of the Argus(®) II device. A sufficiently robust safety profile was demonstrated in the phase I/II clinical trial of 30 patients. Improvement of function in terms of orientation and mobility, target localisation, shape and object recognition, and reading of letters and short unrehearsed words have also been shown. There remains a wide variability in the functional outcomes amongst the patients and the factors contributing to these performance differences are still unclear. Future developments in terms of both software and hardware aimed at improving visual function have been proposed. Further experience in clinical outcomes is being acquired due to increasing implantation. Copyright © 2015. Published by Elsevier Ltd.

  15. Intelligent control of HVAC systems. Part II: perceptron performance analysis

    Directory of Open Access Journals (Sweden)

    Ioan URSU

    2013-09-01

    Full Text Available This is the second part of a paper on intelligent type control of Heating, Ventilating, and Air-Conditioning (HVAC systems. The whole study proposes a unified approach in the design of intelligent control for such systems, to ensure high energy efficiency and air quality improving. In the first part of the study it is considered as benchmark system a single thermal space HVAC system, for which it is assigned a mathematical model of the controlled system and a mathematical model(algorithm of intelligent control synthesis. The conception of the intelligent control is of switching type, between a simple neural network, a perceptron, which aims to decrease (optimize a cost index,and a fuzzy logic component, having supervisory antisaturating role for neuro-control. Based on numerical simulations, this Part II focuses on the analysis of system operation in the presence only ofthe neural control component. Working of the entire neuro-fuzzy system will be reported in a third part of the study.

  16. Purification of yeast alcohol dehydrogenase by using immobilized metal affinity cryogels

    Energy Technology Data Exchange (ETDEWEB)

    Akduman, Begüm [Chemistry Department, Adnan Menderes University, Aydın (Turkey); Uygun, Murat [Koçarlı Vocational and Training School, Adnan Menderes University, Aydın (Turkey); Uygun, Deniz Aktaş, E-mail: daktas@adu.edu.tr [Chemistry Department, Adnan Menderes University, Aydın (Turkey); Akgöl, Sinan [Biochemistry Department, Ege University, İzmir (Turkey); Denizli, Adil [Chemistry Department, Hacettepe University, Ankara (Turkey)

    2013-12-01

    In this study, poly(2-hydroxyethyl methacrylate–glycidylmethacrylate) [poly(HEMA–GMA)] cryogels were prepared by radical cryocopolymerization of HEMA with GMA as a functional comonomer and N,N′-methylene-bisacrylamide (MBAAm) as a crosslinker. Iminodiacetic acid (IDA) functional groups were attached via ring opening of the epoxy group on the poly(HEMA–GMA) cryogels and then Zn(II) ions were chelated with these structures. Characterization of cryogels was performed by FTIR, SEM, EDX and swelling studies. These cryogels have interconnected pores of 30–50 μm size. The equilibrium swelling degree of Zn(II) chelated poly(HEMA–GMA)-IDA cryogels was approximately 600%. Zn(II) chelated poly(HEMA–GMA)-IDA cryogels were used in the adsorption of alcohol dehydrogenase from aqueous solutions and adsorption was performed in continuous system. The effects of pH, alcohol dehydrogenase concentration, temperature, and flow rate on adsorption were investigated. The maximum amount of alcohol dehydrogenase adsorption was determined to be 9.94 mg/g cryogel at 1.0 mg/mL alcohol dehydrogenase concentration and in acetate buffer at pH 5.0 with a flow rate of 0.5 mL/min. Desorption of adsorbed alcohol dehydrogenase was carried out by using 1.0 M NaCI at pH 8.0 phosphate buffer and desorption yield was found to be 93.5%. Additionally, these cryogels were used for purification of alcohol dehydrogenase from yeast with a single-step. The purity of desorbed alcohol dehydrogenase was shown by silver-stained SDS–PAGE. This purification process can successfully be used for the purification of alcohol dehydrogenase from unclarified yeast homogenates and this work is the first report about the usage of the cryogels for purification of alcohol dehydrogenase. - Highlights: • Poly(HEMA–GMA) cryogels were synthesized by radical cryocopolymerization technique. • Prepared cryogels were functionalized with IDA, then Zn(II) ions were chelated to the cryogel. • Zn(II) chelated poly

  17. Purification of yeast alcohol dehydrogenase by using immobilized metal affinity cryogels

    International Nuclear Information System (INIS)

    Akduman, Begüm; Uygun, Murat; Uygun, Deniz Aktaş; Akgöl, Sinan; Denizli, Adil

    2013-01-01

    In this study, poly(2-hydroxyethyl methacrylate–glycidylmethacrylate) [poly(HEMA–GMA)] cryogels were prepared by radical cryocopolymerization of HEMA with GMA as a functional comonomer and N,N′-methylene-bisacrylamide (MBAAm) as a crosslinker. Iminodiacetic acid (IDA) functional groups were attached via ring opening of the epoxy group on the poly(HEMA–GMA) cryogels and then Zn(II) ions were chelated with these structures. Characterization of cryogels was performed by FTIR, SEM, EDX and swelling studies. These cryogels have interconnected pores of 30–50 μm size. The equilibrium swelling degree of Zn(II) chelated poly(HEMA–GMA)-IDA cryogels was approximately 600%. Zn(II) chelated poly(HEMA–GMA)-IDA cryogels were used in the adsorption of alcohol dehydrogenase from aqueous solutions and adsorption was performed in continuous system. The effects of pH, alcohol dehydrogenase concentration, temperature, and flow rate on adsorption were investigated. The maximum amount of alcohol dehydrogenase adsorption was determined to be 9.94 mg/g cryogel at 1.0 mg/mL alcohol dehydrogenase concentration and in acetate buffer at pH 5.0 with a flow rate of 0.5 mL/min. Desorption of adsorbed alcohol dehydrogenase was carried out by using 1.0 M NaCI at pH 8.0 phosphate buffer and desorption yield was found to be 93.5%. Additionally, these cryogels were used for purification of alcohol dehydrogenase from yeast with a single-step. The purity of desorbed alcohol dehydrogenase was shown by silver-stained SDS–PAGE. This purification process can successfully be used for the purification of alcohol dehydrogenase from unclarified yeast homogenates and this work is the first report about the usage of the cryogels for purification of alcohol dehydrogenase. - Highlights: • Poly(HEMA–GMA) cryogels were synthesized by radical cryocopolymerization technique. • Prepared cryogels were functionalized with IDA, then Zn(II) ions were chelated to the cryogel. • Zn(II) chelated poly

  18. Optical system for laser triggering of PBFA II

    International Nuclear Information System (INIS)

    Hamil, R.A.; Seamons, L.O.; Schanwald, L.P.; Gerber, R.A.

    1985-01-01

    The PBFA II laser triggering optical system consists of nearly 300 optical components. These optics must be sufficiently precise to preserve the laser beam quality, as well as to equally distribute the energy of the UV laser beam to the 36, 5.5 MV gas-filled switches at precisely the same instant. Both index variation and cleanliness of the air long the laser path must be controlled. The manual alignment system is capable of alignment to better than the acceptable error of 200 microradians (laser to switches). A technique has been devised to ease the alignment procedure by using a special high gain video camera and a tool alignment telescope to view retroreflective tape targets having optical brightness gains over white surfaces of 10/sup 3/. The camera is a charge-coupled detector intensified by a double microchannel plate having an optical gain of between 10/sup 4/ and 10/sup 5/

  19. The NSLS-II Multilayer Laue Lens Deposition System

    International Nuclear Information System (INIS)

    Conley, R.; Bouet, N.; Biancarosa, J.; Shen, Q.; Boas, L.; Feraca, J.; Rosenbaum, L.

    2009-01-01

    The NSLS-II(1) program has a requirement for an unprecedented level of x-ray nanofocusing and has selected the wedged multilayer Laue lens(2,3) (MLL) as the optic of choice to meet this goal. In order to fabricate the MLL a deposition system is required that is capable of depositing depth-graded and laterally-graded multilayers with precise thickness control over many thousands of layers, with total film growth in one run up to 100 m thick or greater. This machine design expounds on the positive features of a rotary deposition system(4) constructed previously for MLLs and will contain multiple stationary, horizontally-oriented magnetron sources where a transport will move a substrate back and forth in a linear fashion over shaped apertures at well-defined velocities to affect a multilayer coating.

  20. The data acquisition system of the Belle II Pixel Detector

    Science.gov (United States)

    Münchow, D.; Dingfelder, J.; Geßler, T.; Konorov, I.; Kühn, W.; Lange, S.; Lautenbach, K.; Levit, D.; Liu, Z.; Marinas, C.; Schnell, M.; Spruck, B.; Zhao, J.

    2014-08-01

    At the future Belle II experiment the DEPFET (DEPleted Field Effect Transistor) pixel detector will consist of about 8 million channels and is placed as the innermost detector. Because of its small distance to the interaction region and the high luminosity in Belle II, for a trigger rate of about 30 kHz with an estimated occupancy of about 3 % a data rate of about 22 GB/s is expected. Due to the high data rate, a data reduction factor higher than 30 is needed in order to stay inside the specifications of the event builder. The main hardware to reduce the data rate is a xTCA based Compute Node (CN) developed in cooperation between IHEP Beijing and University Giessen. Each node has as main component a Xilinx Virtex-5 FX70T FPGA and is equipped with 2 × 2 GB RAM , GBit Ethernet and 4 × 6.25 Gb/s optical links. An ATCA carrier board is able to hold up to four CN and supplies high bandwidth connections between the four CNs and to the ATCA backplane. To achieve the required data reduction on the CNs, regions of interest (ROI) are used. These regions are calculated in two independent systems by projecting tracks back to the pixel detector. One is the High Level Trigger (HLT) which uses data from the Silicon Vertex Detector (SVD), a silicon strip detector, and outer detectors. The other is the Data Concentrator (DATCON) which calculates ROIs based on SVD data only, in order to get low momentum tracks. With this information, only PXD data inside these ROIs will be forwarded to the event builder, while data outside of these regions will be discarded. First results of the test beam time in January 2014 at DESY with a Belle II vertex detector prototype and full DAQ chain will be presented.

  1. The BEAST II Experiment at Belle II. Characterization of the commissioning detector system for SuperKEKB

    Energy Technology Data Exchange (ETDEWEB)

    Ahlburg, Patrick; Eyring, Andreas; Filimonov, Viacheslav; Krueger, Hans; Mari, Laura; Marinas, Carlos; Pohl, David-Leon; Wermes, Norbert; Dingfelder, Jochen [University of Bonn (Germany)

    2016-07-01

    Before the upgraded vertex detector for the Belle II experiment at the SuperKEKB collider in Japan will be installed, a dedicated detector system for machine commissioning (BEAST II) will be employed. One of its main objectives is to measure and characterize the different background types in order to ensure a safe environment before the installation of the actual silicon detector systems close to the interaction point. FANGS, a detector system at BEAST II, based on ATLAS-IBL front-end electronics and planar silicon sensors is currently being developed for this purpose. The unique feature of this detector system is the high energy resolution achieved by using an external FPGA clock to sample the time-over-threshold signal, while keeping the excellent timing properties. The complete detector system is presented in this talk.

  2. An efficient co-expression and purification system for the complex of Stx4 and C-terminal domain of Synip

    International Nuclear Information System (INIS)

    Tian Wei; Ma Cong; Liu Yingfang; Xu Tao

    2008-01-01

    Synip and Stx4 complex plays a key role in GLUT4 vesicle trafficking and fusion with plasma membrane. The interaction of Synip with Stx4 prevents interaction of VAMP2 located in GLUT4 vesicle with Stx4 in basal state. Insulin induces the dissociation of the Synip and Stx4 complex, and then triggers VAMP2 to interact with Stx4 to form the SNARE complex, thus promoting the vesicle fusion. In this report, we adopt a novel system for co-expression of the Synip and Stx4 by using two common vectors pGEX6p-1 and pET28a(+) to investigate their expression, purification, and interaction. Through this co-expression system, we successfully co-expressed the Synip and Stx4 complex with high yield, and co-purified at an approximate 1:1 molar ratio with high purity (95%). We also demonstrate that the 1-28 residues of Stx4 are dispensable for interaction with Synip using this co-expression system

  3. Study on the thermodynamics of the gadolinium-hydrogen binary system (H/Gd = 0.0–2.0) and implications to metallic gadolinium purification

    Energy Technology Data Exchange (ETDEWEB)

    Fu, Kai; Li, Guoling [Beijing National Laboratory of Molecular Sciences, State Key Laboratory of Rare Earth Materials Chemistry and Applications, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871 (China); Department of Materials Physics and Chemistry, University of Science and Technology Beijing, NO. 30, Xueyuan Road, Beijing 100083 (China); Li, Jigang [Beijing National Laboratory of Molecular Sciences, State Key Laboratory of Rare Earth Materials Chemistry and Applications, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871 (China); Liu, Yang [Department of Food Science, College of Arts and Science, Beijing Union University, Beijing, 100101 (China); Tian, Wenhuai, E-mail: wenhuaitian@ustb.edu.cn [Department of Materials Physics and Chemistry, University of Science and Technology Beijing, NO. 30, Xueyuan Road, Beijing 100083 (China); Zheng, Jie [Beijing National Laboratory of Molecular Sciences, State Key Laboratory of Rare Earth Materials Chemistry and Applications, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871 (China); Li, Xingguo, E-mail: xgli@pku.edu.cn [Beijing National Laboratory of Molecular Sciences, State Key Laboratory of Rare Earth Materials Chemistry and Applications, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871 (China)

    2016-07-15

    The thermodynamics of the gadolinium-hydrogen (Gd–H) binary system (H/Gd = 0.0–2.0) between 650 and 900 °C is studied by pressure composition isotherm measurement. Significant H dissolution in Gd is observed, up to H/Gd = 0.34 at 650 °C and 0.55 at 900 °C. The metal-rich phase boundary of nonstoichiometric gadolinium dihydride was found to occur with nominal composition of GdH{sub 1.80} at 650 °C and GdH{sub 1.53} at 900 °C. The results are in fairly good agreement with previous experimental work but with improved accuracy. The binary phase diagram is obtained using the CALPHAD method. The thermodynamic study here assists the understanding on the efficient deoxygenation effect by the H in Gd for Gd purification. - Highlights: • PCI measurements with high accuracy has been carried out. • A first assessment of Gd–H system has been carried out by the CALPHAD method. • A set of self-consistent thermodynamic parameters was derived for this system.

  4. Reliability analysis for Atucha II reactor protection system signals

    International Nuclear Information System (INIS)

    Roca, Jose Luis

    1996-01-01

    Atucha II is a 745 MW Argentine Power Nuclear Reactor constructed by ENACE SA, Nuclear Argentine Company for Electrical Power Generation and SIEMENS AG KWU, Erlangen, Germany. A preliminary modular logic analysis of RPS (Reactor Protection System) signals was performed by means of the well known Swedish professional risk and reliability software named Risk-Spectrum taking as a basis a reference signal coded as JR17ER003 which command the two moderator loops valves. From the reliability and behavior knowledge for this reference signal follows an estimation of the reliability for the other 97 RPS signals. Because the preliminary character of this analysis Main Important Measures are not performed at this stage. Reliability is by the statistic value named unavailability predicted. The scope of this analysis is restricted from the measurement elements to the RPS buffer outputs. In the present context only one redundancy is analyzed so in the Instrumentation and Control area there no CCF (Common Cause Failures) present for signals. Finally those unavailability values could be introduced in the failure domain for the posterior complete Atucha II reliability analysis which includes all mechanical and electromechanical features. Also an estimation of the spurious frequency of RPS signals defined as faulty by no trip is performed

  5. Reliability analysis for Atucha II reactor protection system signals

    International Nuclear Information System (INIS)

    Roca, Jose L.

    2000-01-01

    Atucha II is a 745 MW Argentine power nuclear reactor constructed by Nuclear Argentine Company for Electric Power Generation S.A. (ENACE S.A.) and SIEMENS AG KWU, Erlangen, Germany. A preliminary modular logic analysis of RPS (Reactor Protection System) signals was performed by means of the well known Swedish professional risk and reliability software named Risk-Spectrum taking as a basis a reference signal coded as JR17ER003 which command the two moderator loops valves. From the reliability and behavior knowledge for this reference signal follows an estimation of the reliability for the other 97 RPS signals. Because the preliminary character of this analysis Main Important Measures are not performed at this stage. Reliability is by the statistic value named unavailability predicted. The scope of this analysis is restricted from the measurement elements to the RPS buffer outputs. In the present context only one redundancy is analyzed so in the Instrumentation and Control area there no CCF (Common Cause Failures) present for signals. Finally those unavailability values could be introduced in the failure domain for the posterior complete Atucha II reliability analysis which includes all mechanical and electromechanical features. Also an estimation of the spurious frequency of RPS signals defined as faulty by no trip is performed. (author)

  6. Water treatment in the EBR-II steam system

    International Nuclear Information System (INIS)

    Klein, M.A.; Hurst, H.

    1975-01-01

    Boiler-water treatment in the EBR-II steam system consists of demineralizing makeup water and using hydrazine to remove traces of oxygen and morpholine to adjust pH to 8.8-9.2. This treatment is called a ''zero-solids'' method, because the chemical agents and reaction products are either volatile or form water and do not contribute solids to the boiler water. A continuous blowdown is cooled, filtered, and deionized to remove impurities and maintain high purity of the water. If a cooling-water leak occurs, phosphate is added to control scaling, and the ''zero-solids'' eatment is suspended until the leak is repaired. Water streams are sampled at six points to control water purity. Examination of the steam drum and an evaporator show the metal surfaces to be in excellent condition with minimal corrosion. The EBR-II steam-generating plant has accumulated over 85,000 hours of in-service operation and has operated successfully for over ten years with the ''zero-solids'' treatment. (auth)

  7. Blade System Design Study. Part II, final project report (GEC).

    Energy Technology Data Exchange (ETDEWEB)

    Griffin, Dayton A. (DNV Global Energy Concepts Inc., Seattle, WA)

    2009-05-01

    As part of the U.S. Department of Energy's Low Wind Speed Turbine program, Global Energy Concepts LLC (GEC)1 has studied alternative composite materials for wind turbine blades in the multi-megawatt size range. This work in one of the Blade System Design Studies (BSDS) funded through Sandia National Laboratories. The BSDS program was conducted in two phases. In the Part I BSDS, GEC assessed candidate innovations in composite materials, manufacturing processes, and structural configurations. GEC also made recommendations for testing composite coupons, details, assemblies, and blade substructures to be carried out in the Part II study (BSDS-II). The BSDS-II contract period began in May 2003, and testing was initiated in June 2004. The current report summarizes the results from the BSDS-II test program. Composite materials evaluated include carbon fiber in both pre-impregnated and vacuum-assisted resin transfer molding (VARTM) forms. Initial thin-coupon static testing included a wide range of parameters, including variation in manufacturer, fiber tow size, fabric architecture, and resin type. A smaller set of these materials and process types was also evaluated in thin-coupon fatigue testing, and in ply-drop and ply-transition panels. The majority of materials used epoxy resin, with vinyl ester (VE) resin also used for selected cases. Late in the project, testing of unidirectional fiberglass was added to provide an updated baseline against which to evaluate the carbon material performance. Numerous unidirectional carbon fabrics were considered for evaluation with VARTM infusion. All but one fabric style considered suffered either from poor infusibility or waviness of fibers combined with poor compaction. The exception was a triaxial carbon-fiberglass fabric produced by SAERTEX. This fabric became the primary choice for infused articles throughout the test program. The generally positive results obtained in this program for the SAERTEX material have led to its

  8. WASTES II: Waste System Transportation and Economic Simulation. Version II. User's guide

    International Nuclear Information System (INIS)

    Shay, M.R.; Buxbaum, M.E.

    1986-02-01

    The WASTES II model was developed to provide detailed analyses beyond the capabilities of other available models. WASTES uses discrete event simulation techniques to model the generation of commercial spent nuclear fuel, the buildup of spent fuel inventories within the system, and the transportation requirements for the movement of radioactive waste throughout the system. The model is written in FORTRAN 77 as an extension to the SLAM commercial simulation language package. In addition to the pool storage and dry storage located at the reactors, the WASTES model provides a choice of up to ten other storage facilities of four different types. The simulation performed by WASTES may be controlled by a combination of source- and/or destination-controlled transfers that are requested by the code user. The user supplies shipping cask characteristics for truck or rail shipment casks. As part of the facility description, the user specifies which casks the facility can use. Shipments within the system can be user specified to occur optimally, or proximally. Optimized shipping can be used when exactly two destination facilities of the same facility type are open for receipt of fuel. Optimized shipping selects source/destination pairs so that the total shipping distance or total shipping costs in a given year are minimized when both facilities are fully utilized. Proximity shipping sequentially fills the closest facility to the source according to the shipment priorities without regard for the total annual shipments. This results in sub-optimal routing of waste material but can be used to approximate an optimal shipping strategy when more than two facilities of the same type are available to receive waste. WASTES is currently able to analyze each of the commercial spent fuel logistics scenarios specified in the 1985 DOE Mission Plan

  9. Sodium purification in Rapsodie

    International Nuclear Information System (INIS)

    Giraud, B.

    1968-01-01

    This report is one of a series of publications presenting the main results of tests carried out during the start-up of the first french fast neutron reactor: Rapsodie. The article presents the sodium purification techniques used in the reactor cooling circuits both from the constructional point of view and with respect to results obtained during the first years working. (author) [fr

  10. Progress on PEP-II magnet power conversion system

    International Nuclear Information System (INIS)

    Bellomo, P.; Genova, L.; Jackson, T.; Shimer, D.

    1996-01-01

    The various power systems for supplying the PEP-II DC magnets rely exclusively on switchmode conversion, utilizing a variety of means depending on the requirements. All of the larger power supplies, ranging from 10 to 200 kW, are powered from DC sources utilizing rectified 480 V AC. Choppers can be used for the series connected strings, but for smaller groups and individual magnets, inverters driving high-frequency transformers with rectifiers comprise the best approach. All of the various systems use a ''building block'' approach of multiple standard-size units connected in series or parallel to most cost-effectively deal with a great range of voltage and current requirements. Utilization of existing infrastructure from PEP-I has been a cost-effective determinant. Equipment is being purchased either off-the-shelf, through performance specification, or by hardware purchase based on design-through-prototype. The corrector magnet power system, utilizing inexpensive, off-the-shelf, four-quadrant switching motor-controllers, has already proven very reliable: 120 of the total of 900 units have been running on the injection system for four months with no failures

  11. Life system modeling and intelligent computing. Pt. II. Proceedings

    Energy Technology Data Exchange (ETDEWEB)

    Li, Kang; Irwin, George W. (eds.) [Belfast Queen' s Univ. (United Kingdom). School of Electronics, Electrical Engineering and Computer Science; Fei, Minrui; Jia, Li [Shanghai Univ. (China). School of Mechatronical Engineering and Automation

    2010-07-01

    This book is part II of a two-volume work that contains the refereed proceedings of the International Conference on Life System Modeling and Simulation, LSMS 2010 and the International Conference on Intelligent Computing for Sustainable Energy and Environment, ICSEE 2010, held in Wuxi, China, in September 2010. The 194 revised full papers presented were carefully reviewed and selected from over 880 submissions and recommended for publication by Springer in two volumes of Lecture Notes in Computer Science (LNCS) and one volume of Lecture Notes in Bioinformatics (LNBI). This particular volume of Lecture Notes in Computer Science (LNCS) includes 55 papers covering 7 relevant topics. The 56 papers in this volume are organized in topical sections on advanced evolutionary computing theory and algorithms; advanced neural network and fuzzy system theory and algorithms; modeling and simulation of societies and collective behavior; biomedical signal processing, imaging, and visualization; intelligent computing and control in distributed power generation systems; intelligent methods in power and energy infrastructure development; intelligent modeling, monitoring, and control of complex nonlinear systems. (orig.)

  12. Progress on PEP-II magnet power conversion system

    Energy Technology Data Exchange (ETDEWEB)

    Bellomo, P.; Genova, L. [Stanford Linear Accelerator Center, Menlo Park, CA (United States); Jackson, T. [Lawrence Berkeley National Lab., CA (United States); Shimer, D. [Lawrence Livermore National Lab., CA (United States)

    1996-06-04

    The various power systems for supplying the PEP-II DC magnets rely exclusively on switchmode conversion, utilizing a variety of means depending on the requirements. All of the larger power supplies, ranging from 10 to 200 kW, are powered from DC sources utilizing rectified 480 V AC. Choppers can be used for the series connected strings, but for smaller groups and individual magnets, inverters driving high-frequency transformers with rectifiers comprise the best approach. All of the various systems use a ``building block`` approach of multiple standard-size units connected in series or parallel to most cost-effectively deal with a great range of voltage and current requirements. Utilization of existing infrastructure from PEP-I has been a cost-effective determinant. Equipment is being purchased either off-the-shelf, through performance specification, or by hardware purchase based on design-through-prototype. The corrector magnet power system, utilizing inexpensive, off-the-shelf, four-quadrant switching motor-controllers, has already proven very reliable: 120 of the total of 900 units have been running on the injection system for four months with no failures.

  13. System modeling of spent fuel transfers at EBR-II

    International Nuclear Information System (INIS)

    Imel, G.R.; Houshyar, A.

    1994-01-01

    The unloading of spent fuel from the Experimental Breeder Reactor-II (EBR-II) for interim storage and subsequent processing in the Fuel Cycle Facility (FCF) is a multi-stage process, involving complex operations at a minimum of four different facilities at the Argonne National Laboratory-West (ANL-W) site. Each stage typically has complicated handling and/or cooling equipment that must be periodically maintained, leading to both planned and unplanned downtime. A program was initiated in October, 1993 to replace the 330 depleted uranium blanket subassemblies (S/As) with stainless steel reflectors. Routine operation of the reactor for fuels performance and materials testing occurred simultaneously in FY 1994 with the blanket unloading. In the summer of 1994, Congress dictated the October 1, 1994 shutdown of EBR-2. Consequently, all blanket S/As and fueled drivers will be removed from the reactor tank and replaced with stainless steel assemblies (which are needed to maintain a precise configuration within the grid so that the under sodium fuel handling equipment can function). A system modeling effort was conducted to determine the means to achieve the objective for the blanket and fuel unloading program, which under the current plan requires complete unloading of the primary tank of all fueled assemblies in 2 1/2 years. A simulation model of the fuel handling system at ANL-W was developed and used to analyze different unloading scenarios; the model has provided valuable information about required resources and modifications to equipment and procedures. This paper reports the results of this modeling effort

  14. Material and energy balances of an integrated biological hydrogen production and purification system and their implications for its potential to reduce greenhouse gas emissions.

    Science.gov (United States)

    Fukushima, Yasuhiro; Huang, Yu-Jung; Chen, Jhen-Wei; Lin, Hung-Chun; Whang, Liang-Ming; Chu, Hsin; Lo, Young-Chong; Chang, Jo-Shu

    2011-09-01

    The materials and energy in an integrated biological hydrogen production and purification system involving hydrolysis, dark fermentation, photo fermentation, CO2 fixation and anaerobic digestion are balanced by integrating the results from multiple experiments, simulations and the literature. The findings are two fold. First, using 1000 kg rice straw as a substrate, 19.8 kg H2 and 138.0 kg CH4 are obtained. The net energy balance (NEB) and net energy ratio (NER) are -738.4 kWh and 77.8%, respectively, both of which imply an unfavorable energy production system. Opportunities to improve the performance particularly lie in the photo fermentation process. Second, greenhouse gas emissions are evaluated for various options. The results were comparable with the emission inventory of electricity generated from fossil fuels. NEB and NER under a zero-carbon-emission constraint were discussed in detail to clarify completely the implications of the energy and material balances on greenhouse gas emissions. Copyright © 2011 Elsevier Ltd. All rights reserved.

  15. Simultaneous decomplexation in blended Cu(II)/Ni(II)-EDTA systems by electro-Fenton process using iron sacrificing electrodes.

    Science.gov (United States)

    Zhao, Zilong; Dong, Wenyi; Wang, Hongjie; Chen, Guanhan; Tang, Junyi; Wu, Yang

    2018-05-15

    This research explored the application of electro-Fenton (E-Fenton) technique for the simultaneous decomplexation in blended Cu(II)/Ni(II)-EDTA systems by using iron sacrificing electrodes. Standard discharge (0.3 mg L -1 for Cu and 0.1 mg L -1 for Ni in China) could be achieved after 30 min reaction under the optimum conditions (i.e. initial solution pH of 2.0, H 2 O 2 dosage of 6 mL L -1  h -1 , current density of 20 mA/cm 2 , inter-electrode distance of 2 cm, and sulfate electrolyte concentration of 2000 mg L -1 ). The distinct differences in apparent kinetic rate constants (k app ) and intermediate removal efficiencies corresponding to mere and blended systems indicated the mutual promotion effect toward the decomplexation between Cu(II) and Ni(II). Massive accumulation of Fe(Ⅲ) favored the further removal of Cu(II) and Ni(II) by metal ion substitution. Species distribution results demonstrated that the decomplexation of metal-EDTA in E-Fenton process was mainly contributed to the combination of various reactions, including Fenton reaction together with the anodic oxidation, electro-coagulation (E-coagulation) and electrodeposition. Unlike hypophosphite and citrate, the presence of chlorine ion displayed favorable effects on the removal efficiencies of Cu(II) and Ni(II) at low dosage, but facilitated the ammonia nitrogen (NH 4 + -N) removal only at high dosage. Copyright © 2018 Elsevier B.V. All rights reserved.

  16. Cover gas purification experience at KNK

    Energy Technology Data Exchange (ETDEWEB)

    Richard, H; Stade, K Ch [Kernkraftwerk-Betriebsgesellschaft m.b.H., Eggenstein-Leopoldshafen (Germany); Stamm, H H [Institute of Radiochemistry, Nuclear Research Center, Karsruhe (Germany)

    1987-07-01

    KNK II is an experimental, sodium cooled fast breeder reactor. The reactor was operated until 1974 with a thermal core (KNK I). The plant was converted into a fast breeder reactor (KNK II) from 1974 to 1977. The commissioning of KNK II was started in October 1977 with the first fast core KNK 11/1. After 400 effective full power days (EFPD) the reactor was shut down in August 1982. After replacing the complete core by the second fast core KNK 11/2, the plant went into operation again in August 1983. In August 1986 nearly 400 EFPD were achieved with the second fast core. Argon is used as cover gas in the primary and secondary sodium systems of KNK. In former times fresh argon was supplied by a bundle of gas cylinders. Later on a liquid argon supply was installed. Purification of cover gas is done by flushing only. During KNK I operation no fuel failures occurred. The primary cover gas activity was characterized by the formation of Ar-41, only small quantities of fission gas were measured, released from 'tramp uranium'. Therefore, no problems existed during KNK I operation with regard to radioactive gas disposal. However, after start-up of KNK II, several fuel elements failed. Until August 1986, five fuel failures were observed, two in KNK 11/1, and three in KNK 11/2. Sometimes, operation with defective fuel pins caused problems when fission gases leaked into the containment atmosphere, and the access had to be restricted. The purging rate of the primary cover gas was limited by the capacity of the charcoal filters in the delay line. Of all non-radioactive impurities, hydrogen (H{sub z}) and nitrogen (N{sub 2}) were of most importance in the primary cover gas. Main source of both impurities was the ingress of air and atmospheric moisture during handling operations in shutdown periods. An other possible source for hydrogen might be a release from the steel-clad zirconium hydride, used as moderator in the moderated driver fuel elements. Additional nitrogen may diffuse

  17. Separation of xylose and glucose using an integrated membrane system for enzymatic cofactor regeneration and downstream purification

    DEFF Research Database (Denmark)

    Morthensen, Sofie Thage; Sigurdardóttir, Sigyn Björk; Meyer, Anne S.

    2017-01-01

    Mixtures of xylose, glucose and pyruvate were fed to a membrane bioreactor equipped with a charged NF membrane (NTR 7450). Value-added products were obtained in the reactor via enzymatic cofactor-dependent catalysis of glucose to gluconic acid and pyruvate to lactic acid, respectively. The initial...... cofactor (NADH) concentration could be decreased to 10% of the stoichiometric value (relative to glucose) without compromising process time and substrate conversion via i) efficient cofactor regeneration and ii) high retention of cofactor (R=0.98) in the membrane bioreactor. Furthermore, accumulation...

  18. Purification and characterization of a collagenase from Penicillium sp. UCP 1286 by polyethylene glycol-phosphate aqueous two-phase system.

    Science.gov (United States)

    de Albuquerque Wanderley, Maria Carolina; Wanderley Duarte Neto, José Manoel; Campos Albuquerque, Wendell Wagner; de Araújo Viana Marques, Daniela; de Albuquerque Lima, Carolina; da Cruz Silvério, Sara Isabel; de Lima Filho, José Luiz; Couto Teixeira, José António; Porto, Ana Lúcia Figueiredo

    2017-05-01

    Collagenases are proteolytic enzymes capable of degrading both native and denatured collagen, reported to be applied in industrial, medical and biotechnological sectors. Liquid-liquid extraction using aqueous two-phase system (ATPS) is one of the most promising bioseparation techniques, which can substitute difficult solid-liquid separation processes, offering many advantages over conventional methods including low-processing time, low-cost material and low-energy consumption. The collagenase produced by Penicillium sp. UCP 1286 showed a stronger affinity for the bottom salt-rich phase, where the highest levels of collagenolytic activity were observed at the center point runs, using 15.0% (w/w) PEG 3350 g/mol and 12.5% (w/w) phosphate salt at pH 7.0 and concentration. The enzyme was characterized by thermal stability, pH tolerance and effect of inhibitors, showing optimal collagenolytic activity at 37 °C and pH 9.0 and proved to be a serine protease. ATPS showed high efficiency in the collagenase purification, confirmed by a single band in SDS/PAGE, and can in fact be applied as a quick and inexpensive alternative method. Copyright © 2017 Elsevier Inc. All rights reserved.

  19. Interaction of Zn(II) with hematite nanoparticles and microparticles: Part 2. ATR-FTIR and EXAFS study of the aqueous Zn(II)/oxalate/hematite ternary system.

    Science.gov (United States)

    Ha, Juyoung; Trainor, Thomas P; Farges, François; Brown, Gordon E

    2009-05-19

    Sorption of Zn(II) to hematite nanoparticles (HN) (av diam=10.5 nm) and microparticles (HM) (av diam=550 nm) was studied in the presence of oxalate anions (Ox2-(aq)) in aqueous solutions as a function of total Zn(II)(aq) to total Ox2-(aq) concentration ratio (R=[Zn(II)(aq)]tot/[Ox2-(aq)]tot) at pH 5.5. Zn(II) uptake is similar in extent for both the Zn(II)/Ox/HN and Zn(II)/Ox/HM ternary systems and the Zn(II)/HN binary system at [Zn(II)(aq)](tot)system than for the Zn(II)/Ox/HM ternary and the Zn(II)/HN and Zn(II)/HM binary systems at [Zn(II)(aq)]tot>4 mM. In contrast, Zn(II) uptake for the Zn(II)/HM binary system is a factor of 2 greater than that for the Zn(II)/Ox/HM and Zn(II)/Ox/HN ternary systems and the Zn(II)/HN binary system at [Zn(II)(aq)]totternary system at both R values examined (0.16 and 0.68), attenuated total reflectance Fourier transform infrared (ATR-FTIR) results are consistent with the presence of inner-sphere oxalate complexes and outer-sphere ZnOx(aq) complexes, and/or type A ternary complexes. In addition, extended X-ray absorption fine structure (EXAFS) spectroscopic results suggest that type A ternary surface complexes (i.e., >O2-Zn-Ox) are present. In the Zn(II)/Ox/HN ternary system at R=0.15, ATR-FTIR results indicate the presence of inner-sphere oxalate and outer-sphere ZnOx(aq) complexes; the EXAFS results provide no evidence for inner-sphere Zn(II) complexes or type A ternary complexes. In contrast, ATR-FTIR results for the Zn/Ox/HN sample with R = 0.68 are consistent with a ZnOx(s)-like surface precipitate and possibly type B ternary surface complexes (i.e., >O2-Ox-Zn). EXAFS results are also consistent with the presence of ZnOx(s)-like precipitates. We ascribe the observed increase of Zn(II)(aq) uptake in the Zn(II)/Ox/HN ternary system at [Zn(II)(aq)]tot>or=4 mM relative to the Zn(II)/Ox/HM ternary system to formation of a ZnOx(s)-like precipitate at the hematite nanoparticle/water interface.

  20. Nonterminals, homomorphisms and codings in different variations of OL-systems. II. Nondeterministic systems

    DEFF Research Database (Denmark)

    Nielsen, Mogens; Rozenberg, Grzegorz; Salomaa, Arto

    1974-01-01

    Continuing the work begun in Part I of this paper, we consider now variations of nondeterministic OL-systems. The present Part II of the paper contains a systematic classification of the effect of nonterminals, codings, weak codings, nonerasing homomorphisms and homomorphisms for all basic variat...

  1. Extraction and Purification of Flavonoids from Radix Puerariae

    African Journals Online (AJOL)

    and preparations are now on sale. ... significant for optimization of extraction, resin selection and ... and flavonoids as indexes, optimize the proper density. ..... system. Sep Purif Technol. 2008; 62: 590-595. 12. Chen JH, Liau BC, Jong TT.

  2. Electron beam silicon purification

    Energy Technology Data Exchange (ETDEWEB)

    Kravtsov, Anatoly [SIA ' ' KEPP EU' ' , Riga (Latvia); Kravtsov, Alexey [' ' KEPP-service' ' Ltd., Moscow (Russian Federation)

    2014-11-15

    Purification of heavily doped electronic grade silicon by evaporation of N-type impurities with electron beam heating was investigated in process with a batch weight up to 50 kilos. Effective temperature of the melt, an indicative parameter suitable for purification process characterization was calculated and appeared to be stable for different load weight processes. Purified material was successfully approbated in standard CZ processes of three different companies. Each company used its standard process and obtained CZ monocrystals applicable for photovoltaic application. These facts enable process to be successfully scaled up to commercial volumes (150-300 kg) and yield solar grade silicon. (copyright 2014 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim) (orig.)

  3. Expert system applications in support of system diagnostics and prognostics at EBR-II

    International Nuclear Information System (INIS)

    Lehto, W.K.; Gross, K.C.

    1989-01-01

    Expert systems have been developed to aid in the monitoring and diagnostics of the Experimental Breeder Reactor-II (EBR-II) at the Idaho National Engineering Laboratory (INEL) in Idaho Falls, Idaho. Systems have been developed for failed fuel surveillance and diagnostics and reactor coolant pump monitoring and diagnostics. A third project is being done jointly by ANL-W and EG ampersand G Idaho to develop a transient analysis system to enhance overall plant diagnostic and prognostic capability. The failed fuel surveillance and diagnosis system monitors, processes, and interprets information from nine key plant sensors. It displays to the reactor operator diagnostic information needed to make proper decisions regarding technical specification conformance during reactor operation with failed fuel. 8 refs., 9 figs., 2 tabs

  4. Pond systems for the purification and disposal of domestic wastewater from small communities: use, design, operation and maintenance

    CSIR Research Space (South Africa)

    Drews, RJLC

    1983-01-01

    Full Text Available Pond systems are ideally suited for small communities, and for schools, hospitals and other institutions since they are simple and economical to construct, operate and maintain. Numerous pons systems have been designed and commissioned in South...

  5. The systems biology of MHC class II antigen presentation

    NARCIS (Netherlands)

    Paul, Petra

    2012-01-01

    Major histocompatibility class II molecules (MHC class II) are one of the key regulators of adaptive immunity because of their specific expression by professional antigen presenting cells (APC). They present peptides derived from endocytosed material to T helper lymphocytes. Consequently, MHC class

  6. A Novel Aqueous Two Phase System Composed of a Thermo-Separating Polymer and an Organic Solvent for Purification of Thermo-Acidic Amylase Enzyme from Red Pitaya (Hylocereus polyrhizus Peel

    Directory of Open Access Journals (Sweden)

    Mehrnoush Amid

    2014-05-01

    Full Text Available The purification of thermo-acidic amylase enzyme from red pitaya (Hylocereus polyrhizus peel for the first time was investigated using a novel aqueous two-phase system (ATPS consisting of a thermo-separating copolymer and an organic solvent. The effectiveness of different parameters such as molecular weight of the thermo-separating ethylene oxide-propylene oxide (EOPO copolymer and type and concentration of organic solvent on the partitioning behavior of amylase was investigated. In addition, the effects of phase components, volume ratio (VR, pH and crude load of purification factor and yield of amylase were evaluated to achieve the optimum partition conditions of the enzyme. In the novel ATPS method, the enzyme was satisfactorily partitioned into the polymer-rich top phase in the system composed of 30% (w/w EOPO 2500 and 15% (w/w 2-propanol, at a volume ratio of 1.94 and with a crude load scale of 25% (w/w at pH 5.0. Recovery and recycling of components was also measured in each successive step of the ATPS process. The enzyme was successfully recovered by the method with a high purification factor of 14.3 and yield of 96.6% and copolymer was also recovered and recycled at a rate above 97%, making the method was more economical than the traditional ATPS method.

  7. EBR-II argon cooling system restricted fuel handling I and C upgrade

    International Nuclear Information System (INIS)

    Start, S.E.; Carlson, R.B.; Gehrman, R.L.

    1995-01-01

    The instrumentation and control of the Argon Cooling System (ACS) restricted fuel handling control system at Experimental Breeder Reactor II (EBR-II) is being upgraded from a system comprised of many discrete components and controllers to a computerized system with a graphical user interface (GUI). This paper describes the aspects of the upgrade including reasons for the upgrade, the old control system, upgrade goals, design decisions, philosophies and rationale, and the new control system hardware and software

  8. The redshift number density evolution of Mg II absorption systems

    International Nuclear Information System (INIS)

    Chen Zhi-Fu

    2013-01-01

    We make use of the recent large sample of 17 042 Mg II absorption systems from Quider et al. to analyze the evolution of the redshift number density. Regardless of the strength of the absorption line, we find that the evolution of the redshift number density can be clearly distinguished into three different phases. In the intermediate redshift epoch (0.6 ≲ z ≲ 1.6), the evolution of the redshift number density is consistent with the non-evolution curve, however, the non-evolution curve over-predicts the values of the redshift number density in the early (z ≲ 0.6) and late (z ≳ 1.6) epochs. Based on the invariant cross-section of the absorber, the lack of evolution in the redshift number density compared to the non-evolution curve implies the galaxy number density does not evolve during the middle epoch. The flat evolution of the redshift number density tends to correspond to a shallow evolution in the galaxy merger rate during the late epoch, and the steep decrease of the redshift number density might be ascribed to the small mass of halos during the early epoch.

  9. Endoribonuclease type II toxin-antitoxin systems: functional or selfish?

    Science.gov (United States)

    Ramisetty, Bhaskar Chandra Mohan; Santhosh, Ramachandran Sarojini

    2017-07-01

    Most bacterial genomes have multiple type II toxin-antitoxin systems (TAs) that encode two proteins which are referred to as a toxin and an antitoxin. Toxins inhibit a cellular process, while the interaction of the antitoxin with the toxin attenuates the toxin's activity. Endoribonuclease-encoding TAs cleave RNA in a sequence-dependent fashion, resulting in translational inhibition. To account for their prevalence and retention by bacterial genomes, TAs are credited with clinically significant phenomena, such as bacterial programmed cell death, persistence, biofilms and anti-addiction to plasmids. However, the programmed cell death and persistence hypotheses have been challenged because of conceptual, methodological and/or strain issues. In an alternative view, chromosomal TAs seem to be retained by virtue of addiction at two levels: via a poison-antidote combination (TA proteins) and via transcriptional reprogramming of the downstream core gene (due to integration). Any perturbation in the chromosomal TA operons could cause fitness loss due to polar effects on the downstream genes and hence be detrimental under natural conditions. The endoribonucleases encoding chromosomal TAs are most likely selfish DNA as they are retained by bacterial genomes, even though TAs do not confer a direct advantage via the TA proteins. TAs are likely used by various replicons as 'genetic arms' that allow the maintenance of themselves and associated genetic elements. TAs seem to be the 'selfish arms' that make the best use of the 'arms race' between bacterial genomes and plasmids.

  10. Rooftop PV system. Final technical progress report, Phase II

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-08-01

    Under this four-year PV:BONUS Program, ECD and United Solar are developing and demonstrating two new lightweight flexible building integrated Photovoltaic (BIPV) modules specifically designed as exact replacements for conventional asphalt shingles and standing seam metal roofing. These modules can be economically and aesthetically integrated into new residential and commercial buildings, and address the even larger roofing replacement market. The modules are designed to be installed by roofing contractors without special training which minimizes the installation and balance of system costs. The modules will be fabricated from high-efficiency, multiple-junction a-Si alloy solar cells developed by ECD and United Solar. Under the Phase I Program, which ended in March 1994, we developed two different concept designs for rooftop PV modules: (1) the United Solar overlapping (asphalt shingle replacement) shingle-type modules and (2) the ECD metal roof-type modules. We also developed a plan for fabricating, testing and demonstrating these modules. Candidate demonstration sites for our rooftop PV modules were identified and preliminary engineering designs for these demonstrations were developed; a marketing study plan was also developed. The major objectives of the Phase II Program, which started in June 1994 was (1) to develop, test, and qualify these new rooftop modules; (2) to develop mechanical and electrical engineering specifications for the demonstration projects; and (3) to develop a marketing/commercialization plan.

  11. The Belle II PXD data acquisition and reduction system

    Energy Technology Data Exchange (ETDEWEB)

    Lange, Soeren; Gessler, Thomas; Kuehn, Wolfgang; Muenchow, David; Spruck, Bjoern [Giessen Univ. (Germany). 2. Physikalisches Inst.; Lin, Haichuan; Liu, Zhen-An; Xu, Hao; Zhao, Jingzhou [IHEP Beijing (China); Collaboration: Belle II Collaboration

    2013-07-01

    The Belle II DEPFET pixel detector (PXD) will deliver high data rates of up to 21.6 Gbytes/s for 3% detector occupancy. Data of this high rate must be buffered for 5 seconds, corresponding to the HLT (High Level Trigger) latency, and then a region-of-interest (ROI) filter is applied to reduce the data rate by a factor of ≥10 by charged track extrapolation from other detectors (SVD, CDC). The PXD readout system is based upon ATCA (Advanced Telecommunications Architecture). The 3rd PCB iteration uses a concept with a xTCA carrier board (with a Virtex-4 FX60 FPGA for ATCA backplane routing) and 4 AMC modules (each with a Xilinx Virtex-5 FX70T FPGA). The FPGA firmware implementation comprises a receiver core for the high speed optical links (≤6.25 Gbps), a buffer management with lookup of ≤270.000 pointers/s, DDR2 memory write (native port interface, ≥1.5 Gbytes/s), Gigabit ethernet (UDP stack in VHDL) and a parallelized ROI selection algorithm. Test results of all the components are presented.

  12. Caltrans WeatherShare Phase II System: An Application of Systems and Software Engineering Process to Project Development

    Science.gov (United States)

    2009-08-25

    In cooperation with the California Department of Transportation, Montana State University's Western Transportation Institute has developed the WeatherShare Phase II system by applying Systems Engineering and Software Engineering processes. The system...

  13. Evaluation on licensability of KNGR system design (II)

    Energy Technology Data Exchange (ETDEWEB)

    Park, G. C.; Seo, K. R. [Seoul National Univ., Seoul (Korea, Republic of); Kim, J. K. [Hanyang Univ., Seoul (Korea, Republic of)] (and others)

    2001-01-15

    The CE methodology of DBA analysis are reviewed. Though UPTF test is different from the KNGR in the geometrical configuration of the down-corner, this was used as a reference to investigate the validity of CEFLASH and COMPERC-II codes in DBA analysis of KNGR. it revealed that CEFLASH is conservative but COMPERC-II for the Refill and Reflood phase is needed for the detailed investigation on ECC bypass, Entrainment, condensation phenomena, CCFL. The direct bypass rate based on the benchmark problem was quantitatively measured. The test model was scaled by the linear scaling methodology, and the accident conditions were the reflood phase at a CB-DEGB LBLOCA. The initial total air flow rate was determined considering the volume scaling factor (1/1000) on criteria of the KNGR design value. The volume rates of ECC water injected: through the DVI nozzles were changed for several case. The direct bypass ratio was about 22 % on the condition of the KNGR scaled-down air volume flow and ECC water velocity at about 1 m/s. The fast neutron fluence at the Reactor Pressure Vessel(RPV) of KNGR designed for 60 years of lifetime was calculated by Monte Carlo simulations and Discrete Ordinates Method for reactor pressure vessel integrity assessment. KNGR core geometry was modeled on a three-dimensional. In the full-scope Monte Carlo method, the maximum fast neutron flux at inner vessel belt line was estimated as 2.738 x 10{sup 10} neutrons/cm{sup 2}{center_dot}see. In the ROCS+MCNP4B calculation, the maximum flux of 2.769 x 10{sup 10} neutrons/ cm{sup 2}{center_dot}see at the RPV was obtained by tallying neutrons crossing the inner surface of the RPV. In ROCS+TORT Calculation, the maximum flux of 3.190 x 10{sup 10} neutrons/cm{sup 2} {center_dot}see was obtained at inner RPV belt line. The lifetime of KNGR was estimated on the basis of conservative end of life fluence limit value of the ABB-CE System 80+. Approximately, 72 Effective Full Power Years (EFPYs), equivalent to 90 calendar

  14. Hydraulic optimization and modeling of hydro-cyclone-systems for treatment and purification of any kind of waters

    Directory of Open Access Journals (Sweden)

    Spangemacher Lars

    2017-01-01

    Full Text Available Water is an indispensable resource for many purposes and good drinking water quality is essential for mankind. This article is supposed to show the need for mobile water treatment systems and therefore to give an overview of different mobile drinking water systems and the technologies available for obtaining good water quality. The aim is to develop a simple to operate water treatment system with few processing stages such as multi-cyclone-cartridge and reverse osmosis with energy recuperation, while the focus is set on modeling and optimizing of hydrocyclone systems as the first treatment stage.

  15. Hydraulic optimization and modeling of hydro-cyclone-systems for treatment and purification of any kind of waters

    Science.gov (United States)

    Spangemacher, Lars; Fröhlich, Siegmund; Buse, Hauke

    2017-11-01

    Water is an indispensable resource for many purposes and good drinking water quality is essential for mankind. This article is supposed to show the need for mobile water treatment systems and therefore to give an overview of different mobile drinking water systems and the technologies available for obtaining good water quality. The aim is to develop a simple to operate water treatment system with few processing stages such as multi-cyclone-cartridge and reverse osmosis with energy recuperation, while the focus is set on modeling and optimizing of hydrocyclone systems as the first treatment stage.

  16. Purification of functionalized DNA origami nanostructures.

    Science.gov (United States)

    Shaw, Alan; Benson, Erik; Högberg, Björn

    2015-05-26

    The high programmability of DNA origami has provided tools for precise manipulation of matter at the nanoscale. This manipulation of matter opens up the possibility to arrange functional elements for a diverse range of applications that utilize the nanometer precision provided by these structures. However, the realization of functionalized DNA origami still suffers from imperfect production methods, in particular in the purification step, where excess material is separated from the desired functionalized DNA origami. In this article we demonstrate and optimize two purification methods that have not previously been applied to DNA origami. In addition, we provide a systematic study comparing the purification efficacy of these and five other commonly used purification methods. Three types of functionalized DNA origami were used as model systems in this study. DNA origami was patterned with either small molecules, antibodies, or larger proteins. With the results of our work we aim to provide a guideline in quality fabrication of various types of functionalized DNA origami and to provide a route for scalable production of these promising tools.

  17. Purification and characterization of the V1 vasopressin receptor from rat liver

    International Nuclear Information System (INIS)

    Fishman, J.B.; Dickey, B.F.; Attisano, C.; Fine, R.E.

    1987-01-01

    The rat liver V1 vasopressin receptor was purified approximately 21,000-fold from rat liver microsomes. The receptor was solubilized from membranes using the zwitterionic detergent CHAPS (3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate). Since the V1 receptor loses its ability to bind ligand when solubilized, the authors devised a liposome reconstitution system to assay vasopressin binding activity during purification. The purified receptor exhibits a K/sub d/ of 6 nm, when, prior to solubilization, the membranes were exposed to 1 μm vasopressin. This resulted in the association of a pertussis-toxin insensitive guanine-nucleotide binding protein with the receptor during most of the purification procedure. The authors are further characterizing the V1-associated G-proteins. In the absence of this association, the receptor has a K/sub d/ of 30 nM. Crosslinking of 125 I-vasopressin to a partially purified preparation of receptor demonstrated that the receptor had a molecular weight of approximately 68,000 under reducing conditions, and 58,000 under non-reducing conditions. The purification procedure may prove useful in purifying a number of small peptide hormone receptors (e.g., bradykinin, angiotensin II) and perhaps their associated G-proteins as well

  18. Novel Instrumentation for Rocket Propulsion Systems, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — The objective of the proposed SBIR Phase II program is to develop, deploy and deliver novel laser-based instruments that provide rapid, in situ, simultaneous...

  19. Multifunctional Aerogel Thermal Protection Systems for Hypersonic Vehicles, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — The overall objective of the Phase II project is to develop lightweight reinforced aerogel materials for use as the core structural insulation material in...

  20. Recession-Tolerant Sensors for Thermal Protection Systems, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — The Phase II project will develop a suite of diagnostic sensors using Direct Write technology to measure temperature, surface recession depth, and heat flux of an...

  1. Behavior of copper (II )and uranium ( VI) in precipitation chromatography in the system anion exchange resin - hexacyanoferrate (II )

    International Nuclear Information System (INIS)

    Seneda, Jose Antonio

    1997-01-01

    In this work it is shown the efficiency of precipitation chromatography for separation and concentration of metallic elements by using a strong anionic-exchange resin saturated with hexacyanoferrate (II). Metallic cations, like Cu (II) and U (VI), are retained from highly diluted solutions and enriched into the resin, in the form of the correspondent insoluble hexacyanoferrate (II), precipitated inside the resin, which permitted the visual observation of a chromatographic zone on the top of the column. It will be discussed the conditions of sorption and elution of the cations uptake by the resin. This system permits the enrichment of the above mentioned cations onto the resin and offers the possibility of interesting separations as well. (author)

  2. The Tore Supra HeII cryogenic system

    International Nuclear Information System (INIS)

    Claudet, G.

    1991-01-01

    The tokamak TORE SUPRA built by the association EURATOM-CEA is routinely operated with a toroidal magnet made of Niobium Titanium cooled at 1.8 K by pressurized HeII. The paper will remind the reasons for such a choice and will describe the corresponding technical solutions. After several years of operation, the validity of the HeII cooling solution will be discussed on the basis of the actual and long term behaviour

  3. EBR-II [Experimental Breeder Reactor-II] system surveillance using pattern recognition software

    International Nuclear Information System (INIS)

    Mott, J.E.; Radtke, W.H.; King, R.W.

    1986-02-01

    The problem of most accurately determining the Experimental Breeder Reactor-II (EBR-II) reactor outlet temperature from currently available plant signals is investigated. Historically, the reactor outlet pipe was originally instrumented with 8 temperature sensors but, during 22 years of operation, all these instruments have failed except for one remaining thermocouple, and its output had recently become suspect. Using pattern recognition methods to compare values of 129 plant signals for similarities over a 7 month period spanning reconfiguration of the core and recalibration of many plant signals, it was determined that the remaining reactor outlet pipe thermocouple is still useful as an indicator of true mixed mean reactor outlet temperature. Application of this methodology to investigate one specific signal has automatically validated the vast majority of the 129 signals used for pattern recognition and also highlighted a few inconsistent signals for further investigation

  4. HOUSEHOLD PURIFICATION OF FLUORIDE CONTAMINATED MAGADI (TRONA)

    DEFF Research Database (Denmark)

    Nielsen, Joan Maj; Dahi, Elian

    1997-01-01

    Purification of fluoride contaminated magadi is studied using bone char sorption and calcium precipitation. The bone char treatment is found to be workable both in columns and in batches where the magadi is dissolved in water prior to treatment. The concentrations in the solutions were 89 g magadi....../L and 95 and 400 mg F/L respectively in natural and synthetic solutions. The fluoride removal capacities observed were 4.6 mg F/g bone char for the column system and 2.7 mg F/g bone char for the batch system in case of synthetic magadi solution. It is however concluded that the batch system is the best...... treatment method. A procedure for purification of fluoride contaminated magadi at household level is described....

  5. Purification and characterization of enterovirus 71 viral particles produced from vero cells grown in a serum-free microcarrier bioreactor system.

    Directory of Open Access Journals (Sweden)

    Chia-Chyi Liu

    Full Text Available BACKGROUND: Enterovirus 71 (EV71 infections manifest most commonly as a childhood exanthema known as hand-foot-and-mouth disease (HFMD and can cause neurological disease during acute infection. PRINCIPAL FINDING: In this study, we describe the production, purification and characterization of EV71 virus produced from Vero cells grown in a five-liter serum-free bioreactor system containing 5 g/L Cytodex 1 microcarrier. The viral titer was >10(6 TCID(50/mL by 6 days post infection when a MOI of 10(-5 was used at the initial infection. Two EV71 virus fractions were separated and detected when the harvested EV71 virus concentrate was purified by sucrose gradient zonal ultracentrifugation. The EV71 viral particles detected in the 24-28% sucrose fractions had an icosahedral structure 30-31 nm in diameter and had low viral infectivity and RNA content. Three major viral proteins (VP0, VP1 and VP3 were observed by SDS-PAGE. The EV71 viral particles detected in the fractions containing 35-38% sucrose were 33-35 nm in size, had high viral infectivity and RNA content, and were composed of four viral proteins (VP1, VP2, VP3 and VP4, as shown by SDS-PAGE analyses. The two virus fractions were formalin-inactivated and induced high virus neutralizing antibody responses in mouse immunogenicity studies. Both mouse antisera recognized the immunodominant linear neutralization epitope of VP1 (residues 211-225. CONCLUSION: These results provide important information for cell-based EV71 vaccine development, particularly for the preparation of working standards for viral antigen quantification.

  6. Pattern-recognition system application to EBR-II plant-life extension

    International Nuclear Information System (INIS)

    King, R.W.; Radtke, W.H.; Mott, J.E.

    1988-01-01

    A computer-based pattern-recognition system, the System State Analyzer (SSA), is being used as part of the EBR-II plant-life extension program for detection of degradation and other abnormalities in plant systems. The SSA is used for surveillance of the EBR-II primary system instrumentation, primary sodium pumps, and plant heat balances. Early results of this surveillance indicate that the SSA can detect instrumentation degradation and system performance degradation over varying time intervals, and can provide derived signal values to replace signals from failed critical sensors. These results are being used in planning for extended-life operation of EBR-II

  7. Exergy and economic analysis of a pyramid-shaped solar water purification system: Active and passive cases

    International Nuclear Information System (INIS)

    Kianifar, Ali; Zeinali Heris, Saeed; Mahian, Omid

    2012-01-01

    An exergy analysis has been conducted to show the effect of a small fan on the exergy efficiency in a pyramid-shaped solar still. The tests were carried out in Mashhad (36° 36′ N), for two solar still systems. One of them was equipped with a small fan (active system), to enhance the evaporation rate while the other one was tested in passive condition (no fan). To examine the effects of radiation and water depth on exergy efficiency, experiments in two seasons and two different depths of water in the solar still basin were performed. The results show that during summer, active unit has higher exergy efficiency than passive one while in winter there is no considerable difference between the exergy efficiency of the units. Results also reveal that the exergy efficiency is higher when the water depth in the basin is lower. Finally, the economic analysis shows a considerable reduction in production cost of the water (8–9%) when the active system is used. -- Highlights: ► Using a small fan in the solar still; reduces the productive cost of fresh water up to 9%. ► Effects of the fan and basin depth on the exergy efficiency during summer and winter were examined. ► Utilizing an active system will increase the daily productivity of fresh water by 20%.

  8. Adsorption of Zn(II) and Cd(II) ions in batch system by using the Eichhornia crassipes.

    Science.gov (United States)

    Módenes, A N; Espinoza-Quiñones, F R; Borba, C E; Trigueros, D E G; Lavarda, F L; Abugderah, M M; Kroumov, A D

    2011-01-01

    In this work, the displacement effects on the sorption capacities of zinc and cadmium ions of the Eichornia crassipes-type biosorbent in batch binary system has been studied. Preliminary single metal sorption experiments were carried out. An improvement on the Zn(II) and Cd(II) ions removal was achieved by working at 30 °C temperature and with non-uniform biosorbent grain sizes. A 60 min equilibrium time was achieved for both Zn(II) and Cd(II) ions. Furthermore, it was found that the overall kinetic data were best described by the pseudo second-order kinetic model. Classical multi-component adsorption isotherms have been tested as well as a modified extended Langmuir isotherm model, showing good agreement with the equilibrium binary data. Around 0.65 mequiv./g maximum metal uptake associated with the E. crassipes biosorbent was attained and the E. crassipes biosorbent has shown higher adsorption affinity for the zinc ions than for the cadmium ones in the binary system.

  9. Web-based ground loop supervision system for the TJ-II Stellarator

    International Nuclear Information System (INIS)

    Pena, A. de la; Lapayese, F.; Pacios, L.; Carrasco, R.

    2005-01-01

    To minimize electromagnetic interferences in diagnostic and control signals, and to guarantee safe operation of TJ-II, ground loops must be avoided. In order to meet this goal, the whole grounding system of the TJ-II was split into multiple single branches that are connected at a single earth point located near the TJ-II structure in the torus hall. A real-time ground loop supervision system (GLSS) has been designed, manufactured and tested by the TJ-II control group for detecting unintentional short circuits between isolated grounded parts. A web server running on the real-time operating system OS-9 provides remote access to the real-time ground loops measurement. Ground loops monitoring and different operation modes can be configured via any web browser. This paper gives the detailed design of the whole TJ-II ground loop supervision system and its results during its operation

  10. Web-based ground loop supervision system for the TJ-II Stellarator

    Energy Technology Data Exchange (ETDEWEB)

    Pena, A. de la [Asociacion EURATOM-CIEMAT Para Fusion, Avd. Complutense 22, 28040 Madrid (Spain)]. E-mail: a.delapena@ciemat.es; Lapayese, F. [Asociacion EURATOM-CIEMAT Para Fusion, Avd. Complutense 22, 28040 Madrid (Spain); Pacios, L. [Asociacion EURATOM-CIEMAT Para Fusion, Avd. Complutense 22, 28040 Madrid (Spain); Carrasco, R. [Asociacion EURATOM-CIEMAT Para Fusion, Avd. Complutense 22, 28040 Madrid (Spain)

    2005-11-15

    To minimize electromagnetic interferences in diagnostic and control signals, and to guarantee safe operation of TJ-II, ground loops must be avoided. In order to meet this goal, the whole grounding system of the TJ-II was split into multiple single branches that are connected at a single earth point located near the TJ-II structure in the torus hall. A real-time ground loop supervision system (GLSS) has been designed, manufactured and tested by the TJ-II control group for detecting unintentional short circuits between isolated grounded parts. A web server running on the real-time operating system OS-9 provides remote access to the real-time ground loops measurement. Ground loops monitoring and different operation modes can be configured via any web browser. This paper gives the detailed design of the whole TJ-II ground loop supervision system and its results during its operation.

  11. Autonomous acquisition systems for TJ-II: controlling instrumentation with a fourth generation language

    International Nuclear Information System (INIS)

    Sanchez, E.; Portas, A.B.; Vega, J.; Agudo, J.M.; McCarthy, K.J.; Ruiz, M.; Barrera, E.; Lopez, S.

    2004-01-01

    Recently, 536 new acquisition channels, made-up of three different channel types, have been incorporated into the TJ-II data acquisition system (DAQ). Dedicated software has also been developed to permit experimentalists to program and control the data acquisition in these systems. The software has been developed using LabView and runs under the Windows 2000 operating system in both personal computer (PC) and PXI controllers. In addition, LabView software has been developed to control TJ-II VXI channels from a PC using a MXI connection. This new software environment will also aid future integration of acquisition channels into the TJ-II remote participation system. All of these acquisition devices work autonomously and are connected to the TJ-II central server via a local area network. In addition, they can be remotely controlled from the TJ-II control-room using Virtual Network Computing (VNC) software

  12. The computerized reactor period measurement system for China fast burst reactor-II

    International Nuclear Information System (INIS)

    Zhao Wuwen; Jiang Zhiguo

    1996-01-01

    The article simply introduces the hardware, principle, and software of the computerized reactor period measurement system for China Fast Burst Reactor-II (CFBR-II). It also gives the relation between fission yield and pre-reactivity of CFBR-II reactor system of bared reactor with decoupled-component and system of bared reactor with multiple light-material. The computerized measurement system makes the reactor period measurement into automatical and intelligent and also improves the speed and precision of period data on-line process

  13. Computer measurement system of reactor period for China fast burst reactor-II

    International Nuclear Information System (INIS)

    Zhao Wuwen; Jiang Zhiguo

    1997-01-01

    The author simply introduces the hardware, principle, and software of the reactor period computer measure system for China Fast Burst Reactor-II (CFBR-II). It also gives the relation between Fission yield and Pre-reactivity of CFBR-II reactor system of bared reactor with decoupled-component and system of bared reactor with multiple light-material. The computer measure system makes the reactor period measurement into automation and intellectualization and also improves the speed and precision of period data process on-line

  14. A floating cogeneration system using the Russian KLT-40 reactor and Canadian reverse osmosis water purification technology

    International Nuclear Information System (INIS)

    Humphries, J.R.; Davies, K.

    1997-01-01

    As the global consumption of water increases with growing populations and rising levels of industrialization, major new sources of potable water production must be developed. To address this issue efficiently and economically, a new approach has been developed in Canada for the integration of reverse osmosis (RO) desalination systems with nuclear reactors as an energy source. The use of waste heat from the electrical generation process to preheat the RO feedwater, advanced feedwater pre-treatment and sophisticated system design integration and optimization techniques have led to improved water production efficiency, lower water production costs and reduced environmental impacts. CANDESAL Inc., has studied the use of its approach to the application of RO technology in the Russian APWS-80 floating nuclear desalination plant. Case studies show that water production efficiently improvements up to about 16% can be achieved. The energy consumed for the CANDESAL optimized APWS-80 design configuration is 4.2 kW·h/m 3 compared to the base APWS-80 design value of 4.9 kW·h/m 3 . Although only a preliminary study, these results suggest that significant improvements in the cost of water production can be achieved. The potential benefits warrant further detailed evaluation followed by a demonstration project. (author). 1 ref., 6 figs, 2 tabs

  15. EBR-II operating experience

    International Nuclear Information System (INIS)

    Smith, C.R.F.

    1978-07-01

    Operation of the EBR-2 reactor is presented concerning the performance of the heat removal system; reactor materials; fuel handling system; sodium purification and sampling system; cover-gas purification; plant diagnostics and instrumentation; recent improvements in identifying fission product sources in EBR-2; and EBR-2 safety

  16. Kilowatt isotope power system, Phase II Plan. Volume IV. Teledyne FSCD vs GDS

    Energy Technology Data Exchange (ETDEWEB)

    1978-03-15

    This Volume contains Teledyne's input to the Kilowatt Isotope Power System Phase II Plan. Included is a description of the Flight System Heat Generation System, Flight System Radiator, Thermal Insulation Stability, GDS Heat Generation System and GDS Radiator.

  17. Production, purification and immunogenicity of recombinant Ebola virus proteins - A comparison of Freund's adjuvant and adjuvant system 03.

    Science.gov (United States)

    Melén, Krister; Kakkola, Laura; He, Felix; Airenne, Kari; Vapalahti, Olli; Karlberg, Helen; Mirazimi, Ali; Julkunen, Ilkka

    2017-04-01

    There is an urgent need for Ebola virus (EBOV) proteins, EBOV-specific antibodies and recombinant antigens to be used in diagnostics and as potential vaccine candidates. Our objective was to produce and purify recombinant proteins for immunological assays and for the production of polyclonal EBOV specific antibodies. In addition, a limited comparison of the adjuvant effects of Freund's complete adjuvant (FCA) and adjuvant system 03 (AS03) was carried out. Recombinant EBOV GST-VP24, -VP30, -VP35, -VP40 and -NP were produced in E. coli and purified with affinity chromatography followed by preparative gel electrophoresis. Recombinant EBOV GP-His was produced in Sf9 insect cells and purified by preparative gel electrophoresis. To compare the adjuvant effect of FCA and AS03, 12 rabbits were immunized four times with one of the six recombinant EBOV proteins using FCA or AS03. In addition, three guinea pigs were immunized with EBOV VP24 using FCA. With the exception of sera from two rabbits immunized with GST-VP24, the antisera against all other EBOV proteins showed very high and specific antibody responses after three to four immunizations. The adjuvant effect of AS03 was comparable to that of FCA. The produced antibodies recognized the corresponding EBOV proteins in wild type EBOV-infected cells. Copyright © 2016 Elsevier B.V. All rights reserved.

  18. Statistical and Judgmental Criteria for Scale Purification

    DEFF Research Database (Denmark)

    Wieland, Andreas; Durach, Christian F.; Kembro, Joakim

    2017-01-01

    of scale purification, to critically analyze the current state of scale purification in supply chain management (SCM) research and to provide suggestions for advancing the scale-purification process. Design/methodology/approach A framework for making scale-purification decisions is developed and used...

  19. Overview of the purification of recombinant proteins.

    Science.gov (United States)

    Wingfield, Paul T

    2015-04-01

    When the first version of this unit was written in 1995, protein purification of recombinant proteins was based on a variety of standard chromatographic methods and approaches, many of which were described and mentioned throughout Current Protocols in Protein Science. In the interim, there has been a shift toward an almost universal usage of the affinity or fusion tag. This may not be the case for biotechnology manufacture where affinity tags can complicate producing proteins under regulatory conditions. Regardless of the protein expression system, questions are asked as to which and how many affinity tags to use, where to attach them in the protein, and whether to engineer a self-cleavage system or simply leave them on. We will briefly address some of these issues. Also, although this overview focuses on E.coli, protein expression and purification, other commonly used expression systems are mentioned and, apart from cell-breakage methods, protein purification methods and strategies are essentially the same. Copyright © 2015 John Wiley & Sons, Inc.

  20. Functional evidence for alternative ANG II-forming pathways in hamster cardiovascular system

    NARCIS (Netherlands)

    Nishimura, H; Buikema, H; Baltatu, O; Ganten, D; Urata, H

    1998-01-01

    Like human chymase, hamster chymase is an ANG II-forming enzyme, but pathophysiological roles of chymase are still unknown. We determined the functional conversion of ANG I and [Pro(11), D-Ala(12)]ANG I, a chymase-selective substrate, to ANG II in the hamster cardiovascular system. ANG I and

  1. A steerable/distance enhanced penetrometer delivery system: Phase II. Topical report

    International Nuclear Information System (INIS)

    Amini, A.; Shenhar, J.; Lum, K.D.

    1996-05-01

    This report summarizes the phase II work on the Position Location Device (POLO) for penetrometers. Phase II was carried out to generate an integrated design of a full-scale steerable/distance enhanced penetrometer delivery system. Steering provides for the controlled and directional use of the penetrometer, while vibratory thrusting can provide greater penetration ability

  2. ERP II: a conceptual framework for next-generation enterprise systems?

    DEFF Research Database (Denmark)

    Møller, Charles

    2005-01-01

    proposes a conceptual framework for extended enterprise resource planning (ERP II). The aim of this model is to compile present ES concepts into a comprehensive outline of ERP II, thus composing a generic map and taxonomy for corporate-wide enterprise systems. Research limitations/implications - The paper...

  3. 77 FR 58473 - Minimum Technical Standards for Class II Gaming Systems and Equipment

    Science.gov (United States)

    2012-09-21

    ... to all equipment, including computer, electronic, or other technologic aids used with Class II games..., computer, or other technologic aids in connection with the play of Class II games. This part establishes... gaming system, causes a discontinuance of game play or other component functions. Financial instrument...

  4. Future of antibody purification.

    Science.gov (United States)

    Low, Duncan; O'Leary, Rhona; Pujar, Narahari S

    2007-03-15

    Antibody purification seems to be safely ensconced in a platform, now well-established by way of multiple commercialized antibody processes. However, natural evolution compels us to peer into the future. This is driven not only by a large, projected increase in the number of antibody therapies, but also by dramatic improvements in upstream productivity, and process economics. Although disruptive technologies have yet escaped downstream processes, evolution of the so-called platform is already evident in antibody processes in late-stage development. Here we perform a wide survey of technologies that are competing to be part of that platform, and provide our [inherently dangerous] assessment of those that have the most promise.

  5. Affinity chromatography: A versatile technique for antibody purification.

    Science.gov (United States)

    Arora, Sushrut; Saxena, Vikas; Ayyar, B Vijayalakshmi

    2017-03-01

    Antibodies continue to be extremely utilized entities in myriad applications including basic research, imaging, targeted delivery, chromatography, diagnostics, and therapeutics. At production stage, antibodies are generally present in complex matrices and most of their intended applications necessitate purification. Antibody purification has always been a major bottleneck in downstream processing of antibodies, due to the need of high quality products and associated high costs. Over the years, extensive research has focused on finding better purification methodologies to overcome this holdup. Among a plethora of different techniques, affinity chromatography is one of the most selective, rapid and easy method for antibody purification. This review aims to provide a detailed overview on affinity chromatography and the components involved in purification. An array of support matrices along with various classes of affinity ligands detailing their underlying working principles, together with the advantages and limitations of each system in purifying different types of antibodies, accompanying recent developments and important practical methodological considerations to optimize purification procedure are discussed. Copyright © 2016 Elsevier Inc. All rights reserved.

  6. Commercial ampholytes used for isoelectric focusing may interfere with bioactivity based purification of antimicrobial peptides

    OpenAIRE

    Riazi, Shadi; Dover, Sara; Turovskiy, Yevgeniy; Chikindas, Michael L.

    2007-01-01

    BioRad's Rotofor® system has been frequently used for the purification of proteins and smaller peptides such as bacteriocins. In this study, we report that some commercially available ampholytes used with the Rotofor® isoelectric focusing system possess antimicrobial activity, which may interfere with the purification of bacteriocins and bacteriocin-like substances.

  7. Computer control system of the cooler-synchrotron TARN-II

    International Nuclear Information System (INIS)

    Watanabe, S.; Watanabe, T.; Yoshizawa, M.; Katayama, T.

    1993-11-01

    The client-server model enables us to develop the flexible control system such as a TARN-II computer control system. The system forms a single machine including a message bus to communicate between them. An auxiliary control path in the client-server model serves a high speed device control. The configuration and performance of that control system are described. (author)

  8. Autonomous Ocean Sampling Networks II (AOSN-II): System Engineering and Project Coordination

    National Research Council Canada - National Science Library

    Bellingham, James

    2003-01-01

    .... Over 21 different autonomous robotic systems, three ships, an aircraft, CODAR, drifters, floats, and numerous moored observation assets were used in the field program to produce an unprecedented data...

  9. Solid State Transmitters for Water Vapor and Ozone DIAL Systems, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — The focus of this Select Phase II program is to build and deliver laser components both for airborne water vapor and ozone DIAL systems. Specifically, Fibertek...

  10. Novel Left Ventricular Assist Systems® I and II for Cardiac Recovery: The Driver

    OpenAIRE

    Cervino, Claudio; Nasini, Victor; Sroka, Agnieszka; Diluch, Armando; Cáceres, Miguel; Sellanes, Miguel; Malusardi, Adriano; del Rio, Miguel; Pham, Si M.; Liotta, Domingo

    2005-01-01

    We have recently described the Novel Left Ventricular Assist Systems® (Novel LVAS®) I and II, which avoid cannulation of cardiac chambers and synchronize pumping with the patient's electrocardiogram.

  11. Purification of a-galactosidase from seeds of Sesbania marginata

    Directory of Open Access Journals (Sweden)

    Falco A.L.P.

    2000-01-01

    Full Text Available Alpha-galactosidase taken from a raw extract of Sesbania marginata legume seeds was purified by partitioning in aqueous two-phase systems (ATPS. Initially, galactomannan/dextran 2,000,000 systems were used for the purification, and the partition coefficients of alpha -galactosidase varied from 1.5 to 4.0. However, mass transport in these systems was poor due to the high viscosity of the employed polymers. Therefore, partitioning in polyethyleneglycol (PEG/ sodium phosphate systems and the effect of sodium chloride upon the enzyme purification and the yield of alpha -galactosidase were also investigated. The purification achieved in a single-step was 5.7 with a recovery of 144% of alpha -galactosidase, possibly due to the removal of materials which inhibited alpha -galactosidase activity before the purification. The removal of the main protein contaminants and the highest yields were achieved in PEG 4,000/ sodium phosphate + 6% NaCl system at pH 5.0. Further purification by preparative on-exchange chromatography was also developed.

  12. 25 CFR 547.6 - What are the minimum technical standards for enrolling and enabling Class II gaming system...

    Science.gov (United States)

    2010-04-01

    ... and enabling Class II gaming system components? 547.6 Section 547.6 Indians NATIONAL INDIAN GAMING COMMISSION, DEPARTMENT OF THE INTERIOR HUMAN SERVICES MINIMUM TECHNICAL STANDARDS FOR GAMING EQUIPMENT USED... enabling Class II gaming system components? (a) General requirements. Class II gaming systems shall provide...

  13. PMT response drift of ATLAS Tile Laser II calibration system: an introduction of a new method

    CERN Document Server

    Di Gregorio, Giulia

    2016-01-01

    In this article I describe the performance of the monitoring diodes of the Laser II system, a new system for run II used to calibrate the gain variation of PMTs in between two cesium scan. I also show a new method to measure the PMT drift response that it is compared to the method used up to now (Clermont-Ferrant) corrected with the Pisa method. The agreement between the two method is within 0.2%.

  14. A cryogenic system for TIBER II [Tokamak Ignition/Burn Experimental Reactor

    International Nuclear Information System (INIS)

    Slack, D.S.; Kerns, J.A.

    1987-01-01

    Phase II of the Tokamak Ignition/Burn Experimental Reactor (TIBER II) study describes one option for a small, economical, next-generation tokamak [1,2]. Because of its small size, minimum shielding is used between the plasma and the toroidal-field (TF) coils. Consequently, a large cryogenic system (approximately 70 kW at 4.5 K) capable of delivering forced-flow helium is required. This paper describes a cryogenic system that meets this requirement and includes TIBER-II requirements. 3 refs

  15. A portable data acquisition system on J.I.P.P. T-II ICRF experiment

    International Nuclear Information System (INIS)

    Hidekuma, S.

    1982-03-01

    This system has been developed for the data acquisition in the J.I.P.P. T-II ICRF experiment. It is composed of the LSI-11/2(56KB), a dual floppy disk drive, CAMAC modules, a graphic display and an interface module to the HITAC 10-II system. The operating system is RT-11. This system has functions of the data acquisition through A-D converters (max.32ch), the transfer of the data to the HITAC 10-II system and the preservation of them in its floppy disk. Furthermore, a user can easily develop his application programs with this system. The operating procedures of this system are described. (author)

  16. Online Oxide Contamination Measurement and Purification Demonstration

    Science.gov (United States)

    Bradley, D. E.; Godfroy, T. J.; Webster, K. L.; Garber, A. E.; Polzin, K. A.; Childers, D. J.

    2011-01-01

    Liquid metal sodium-potassium (NaK) has advantageous thermodynamic properties indicating its use as a fission reactor coolant for a surface (lunar, martian) power system. A major area of concern for fission reactor cooling systems is system corrosion due to oxygen contaminants at the high operating temperatures experienced. A small-scale, approximately 4-L capacity, simulated fission reactor cooling system employing NaK as a coolant was fabricated and tested with the goal of demonstrating a noninvasive oxygen detection and purification system. In order to generate prototypical conditions in the simulated cooling system, several system components were designed, fabricated, and tested. These major components were a fully-sealed, magnetically-coupled mechanical NaK pump, a graphite element heated reservoir, a plugging indicator system, and a cold trap. All system components were successfully demonstrated at a maximum system flow rate of approximately 150 cc/s at temperatures up to 550 C. Coolant purification was accomplished using a cold trap before and after plugging operations which showed a relative reduction in oxygen content.

  17. How much DNA is lost? Measuring DNA loss of short-tandem-repeat length fragments targeted by the PowerPlex 16® system using the Qiagen MinElute Purification Kit.

    Science.gov (United States)

    Kemp, Brian M; Winters, Misa; Monroe, Cara; Barta, Jodi Lynn

    2014-01-01

    The success in recovering genetic profiles from aged and degraded biological samples is diminished by fundamental aspects of DNA extraction, as well as its long-term preservation, that are not well understood. While numerous studies have been conducted to determine whether one extraction method was superior to others, nearly all of them were initiated with no knowledge of the actual starting DNA quantity in the samples prior to extraction, so they ultimately compared the outcome of all methods relative to the best. Using quantitative PCR to estimate the copy count of synthetic standards before (i.e., "copies in") and after (i.e., "copies out") purification by the Qiagen MinElute PCR Purification Kit, we documented DNA loss within a pool of 16 different-sized fragments ranging from 106 to 409 bp in length, corresponding to those targeted by the PowerPlex 16 System (Promega, Madison, WI). Across all standards from 10(4) to 10(7) copies/μL, loss averaged between 21.75% and 60.56% (mean, 39.03%), which is not congruent with Qiagen's claim that 80% of 70 bp to 4 kb fragments are retained using this product (i.e., 20% loss). Our study also found no clear relationship either between DNA strand length and retention or between starting copy number and retention. This suggests that there is no molecule bias across the MinElute column membrane and highlights the need for manufacturers to clearly and accurately describe on what their claims are based, and should also encourage researchers to document DNA retention efficiencies of their own methods and protocols. Understanding how and where to reduce loss of molecules during extraction and purification will serve to generate clearer and more accurate data, which will enhance the utility of ancient and low-copy-number DNA as a tool for closing forensic cases or in reconstructing the evolutionary history of humans and other organisms.

  18. Immobilized palladium(II) ion affinity chromatography for recovery of recombinant proteins with peptide tags containing histidine and cysteine.

    Science.gov (United States)

    Kikot, Pamela; Polat, Aise; Achilli, Estefania; Fernandez Lahore, Marcelo; Grasselli, Mariano

    2014-11-01

    Fusion of peptide-based tags to recombinant proteins is currently one of the most used tools for protein production. Also, immobilized metal ion affinity chromatography (IMAC) has a huge application in protein purification, especially in research labs. The combination of expression systems of recombinant tagged proteins with this robust chromatographic system has become an efficient and rapid tool to produce milligram-range amounts of proteins. IMAC-Ni(II) columns have become the natural partners of 6xHis-tagged proteins. The Ni(II) ion is considered as the best compromise of selectivity and affinity for purification of a recombinant His-tagged protein. The palladium(II) ion is also able to bind to side chains of amino acids and form ternary complexes with iminodiacetic acid and free amino acids and other sulfur-containing molecules. In this work, we evaluated two different cysteine- and histidine-containing six amino acid tags linked to the N-terminal group of green fluorescent protein (GFP) and studied the adsorption and elution conditions using novel eluents. Both cysteine-containing tagged GFPs were able to bind to IMAC-Pd(II) matrices and eluted successfully using a low concentration of thiourea solution. The IMAC-Ni(II) system reaches less than 20% recovery of the cysteine-containing tagged GFP from a crude homogenate of recombinant Escherichia coli, meanwhile the IMAC-Pd(II) yields a recovery of 45% with a purification factor of 13. Copyright © 2014 John Wiley & Sons, Ltd.

  19. Moderator purification and design modifications based on operation feedback

    Energy Technology Data Exchange (ETDEWEB)

    Das, S; Chakrabarti, A K; Shirolkar, K M; Sharma, V K [Nuclear Power Corporation, Bhabha Atomic Research Centre, Mumbai (India)

    1994-06-01

    Heavy water is used as a moderator in the Pressurized Heavy Water Reactors using natural uranium as a fissile fuel. The purification system is provided to maintain the purity of moderator heavy water so as to minimise the radiolytic decomposition of heavy water due to nuclear radiation which otherwise would lead to hazardous concentration of deuterium in the moderator cover gas. The presence of dissolved impurity in the moderator increases the radiolysis rate by impeding the reverse reaction and hence these must be removed. The purification system in general controls the chemistry of moderator by minimizing the corrosion of piping in the circuit and along with the liquid poison injection system adjusts the concentration of the poisons in the moderator. This paper describes the evolution of the purification system for the 500 MWe PHWRs based on various operating requirements and feedback from the operating stations. (author). 2 refs., 3 figs., 1 tab.

  20. Moderator purification and design modifications based on operation feedback

    International Nuclear Information System (INIS)

    Das, S.; Chakrabarti, A.K.; Shirolkar, K.M.; Sharma, V.K.

    1994-01-01

    Heavy water is used as a moderator in the Pressurized Heavy Water Reactors using natural uranium as a fissile fuel. The purification system is provided to maintain the purity of moderator heavy water so as to minimise the radiolytic decomposition of heavy water due to nuclear radiation which otherwise would lead to hazardous concentration of deuterium in the moderator cover gas. The presence of dissolved impurity in the moderator increases the radiolysis rate by impeding the reverse reaction and hence these must be removed. The purification system in general controls the chemistry of moderator by minimizing the corrosion of piping in the circuit and along with the liquid poison injection system adjusts the concentration of the poisons in the moderator. This paper describes the evolution of the purification system for the 500 MWe PHWRs based on various operating requirements and feedback from the operating stations. (author)

  1. Rotating Reverse-Osmosis for Water Purification

    Science.gov (United States)

    Lueptow, RIchard M.

    2004-01-01

    A new design for a water-filtering device combines rotating filtration with reverse osmosis to create a rotating reverse- osmosis system. Rotating filtration has been used for separating plasma from whole blood, while reverse osmosis has been used in purification of water and in some chemical processes. Reverse- osmosis membranes are vulnerable to concentration polarization a type of fouling in which the chemicals meant not to pass through the reverse-osmosis membranes accumulate very near the surfaces of the membranes. The combination of rotating filtration and reverse osmosis is intended to prevent concentration polarization and thereby increase the desired flux of filtered water while decreasing the likelihood of passage of undesired chemical species through the filter. Devices based on this concept could be useful in a variety of commercial applications, including purification and desalination of drinking water, purification of pharmaceutical process water, treatment of household and industrial wastewater, and treatment of industrial process water. A rotating filter consists of a cylindrical porous microfilter rotating within a stationary concentric cylindrical outer shell (see figure). The aqueous suspension enters one end of the annulus between the inner and outer cylinders. Filtrate passes through the rotating cylindrical microfilter and is removed via a hollow shaft. The concentrated suspension is removed at the end of the annulus opposite the end where the suspension entered.

  2. Evaluation of Laser Stabilization and Imaging Systems for LCLS-II - Final Paper

    Energy Technology Data Exchange (ETDEWEB)

    Barry, Matthew [Auburn Univ., AL (United States)

    2015-08-20

    By combining the top performing commercial laser beam stabilization system with the most ideal optical imaging configuration, the beamline for the Linear Accelerator Coherent Light Source II (LCLS-II) will deliver the highest quality and most stable beam to the cathode. To determine the optimal combination, LCLS-II beamline conditions were replicated and the systems tested with a He-Ne laser. The Guidestar-II and MRC active laser beam stabilization systems were evaluated for their ideal positioning and stability. Both a two and four lens optical imaging configuration was then evaluated for beam imaging quality, magnification properties, and natural stability. In their best performances when tested over fifteen hours, Guidestar-II kept the beam stable over approximately 70-110um while the MRC system kept it stable over approximately 90-100um. During short periods of time, Guidestar-II kept the beam stable between 10-20um, but was more susceptible to drift over time, while the MRC system maintained the beam between 30-50um with less overall drift. The best optical imaging configuration proved to be a four lens system that images to the iris located in the cathode room and from there, imaged to the cathode. The magnification from the iris to the cathode was 2:1, within an acceptable tolerance to the expected 2.1:1 magnification. The two lens configuration was slightly more stable in small periods of time (less than 10 minutes) without the assistance of a stability system, approximately 55um compared to approximately 70um, but the four lens configurations beam image had a significantly flatter intensity distribution compared to the two lens configuration which had a Gaussian distribution. A final test still needs to be run with both stability systems running at the same time through the four lens system. With this data, the optimal laser beam stabilization system can be determined for the beamline of LCLS-II.

  3. Sorption of Hg(II and Pb(II Ions on Chitosan-Iron(III from Aqueous Solutions: Single and Binary Systems

    Directory of Open Access Journals (Sweden)

    Byron Lapo

    2018-03-01

    Full Text Available The present work describes the study of mercury Hg(II and lead Pb(II removal in single and binary component systems into easily prepared chitosan-iron(III bio-composite beads. Scanning electron microscopy and energy-dispersive X-ray (SEM-EDX analysis, Fourier transform infrared spectroscopy (FTIR, thermogravimetric analysis (TGA and point of zero charge (pHpzc analysis were carried out. The experimental set covered pH study, single and competitive equilibrium, kinetics, chloride and sulfate effects as well as sorption–desorption cycles. In single systems, the Langmuir nonlinear model fitted the experimental data better than the Freundlich and Sips equations. The sorbent material has more affinity to Hg(II rather than Pb(II ions, the maximum sorption capacities were 1.8 mmol·g−1 and 0.56 mmol·g−1 for Hg(II and Pb(II, respectively. The binary systems data were adjusted with competitive Langmuir isotherm model. The presence of sulfate ions in the multicomponent system [Hg(II-Pb(II] had a lesser impact on the sorption efficiency than did chloride ions, however, the presence of chloride ions improves the selectivity towards Hg(II ions. The bio-based material showed good recovery performance of metal ions along three sorption–desorption cycles.

  4. Magnesium Hall Thruster for Solar System Exploration, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — The innovation being developed in this program is a Mg Hall Effect Thruster system that would open the door for In-Situ Resource Utilization based solar system...

  5. MOIDSS?- Mobile Online Intelligent Decision Support System, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — GRID has had a successfully completed Phase I 'Mobile Online Intelligent Decision Support System' (MOIDSS). The system developed into a total solution that supports...

  6. Distributed Rocket Engine Testing Health Monitoring System, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — Leveraging the Phase I achievements of the Distributed Rocket Engine Testing Health Monitoring System (DiRETHMS) including its software toolsets and system building...

  7. Novel Color Depth Mapping Imaging Sensor System, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — Autonomous and semi-autonomous robotic systems require information about their surroundings in order to navigate properly. A video camera machine vision system can...

  8. Ocean Thermal Energy Conservation (OTEC) power system development (PDS) II. Preliminary design report

    Energy Technology Data Exchange (ETDEWEB)

    1979-08-10

    This report documents the results and conclusions of the PDS II, Phase I, preliminary design of a 10 MWe OTEC power system, using enhanced plate type heat exchangers, and of representative 0.2 MWe test articles. It further provides the documentation (specifications, drawings, trade studies, etc.) resulting from the design activities. The data and discussions of the technical concepts are organized to respond to the PDS II, Phase II proposal evaluation criteria. This volume, which specifically addresses the three evaluation categories (heat exchangers, rotating machinery, and power system configuration and performance) is an integral part of the Phase II plans (proposal) which describe the technical approach to delivering test articles to OTEC-1. In addition, there is a section which addresses power system cost and net energy analysis and another which discusses the results of stainless steel feasibility studies. Supporting documentation is contained in two appendix volumes.

  9. Topological structures of complex belief systems (II): Textual materialization

    OpenAIRE

    Nescolarde Selva, Josué; Usó i Domènech, Josep Lluís

    2013-01-01

    Mythical and religious belief systems in a social context can be regarded as a conglomeration of sacrosanct rites, which revolve around substantive values that involve an element of faith. Moreover, we can conclude that ideologies, myths and beliefs can all be analyzed in terms of systems within a cultural context. The significance of being able to define ideologies, myths and beliefs as systems is that they can figure in cultural explanations. This, in turn, means that such systems can figur...

  10. Five-Year Safety and Performance Results from the Argus II Retinal Prosthesis System Clinical Trial.

    Science.gov (United States)

    da Cruz, Lyndon; Dorn, Jessy D; Humayun, Mark S; Dagnelie, Gislin; Handa, James; Barale, Pierre-Olivier; Sahel, José-Alain; Stanga, Paulo E; Hafezi, Farhad; Safran, Avinoam B; Salzmann, Joel; Santos, Arturo; Birch, David; Spencer, Rand; Cideciyan, Artur V; de Juan, Eugene; Duncan, Jacque L; Eliott, Dean; Fawzi, Amani; Olmos de Koo, Lisa C; Ho, Allen C; Brown, Gary; Haller, Julia; Regillo, Carl; Del Priore, Lucian V; Arditi, Aries; Greenberg, Robert J

    2016-10-01

    The Argus II Retinal Prosthesis System (Second Sight Medical Products, Inc, Sylmar, CA) was developed to restore some vision to patients blind as a result of retinitis pigmentosa (RP) or outer retinal degeneration. A clinical trial was initiated in 2006 to study the long-term safety and efficacy of the Argus II System in patients with bare or no light perception resulting from end-stage RP. Prospective, multicenter, single-arm clinical trial. Within-patient controls included the nonimplanted fellow eye and patients' native residual vision compared with their vision with the Argus II. Thirty participants in 10 centers in the United States and Europe. The worse-seeing eye of blind patients was implanted with the Argus II. Patients wore glasses mounted with a small camera and a video processor that converted images into stimulation patterns sent to the electrode array on the retina. The primary outcome measures were safety (the number, seriousness, and relatedness of adverse events) and visual function, as measured by 3 computer-based, objective tests. Secondary measures included functional vision performance on objectively scored real-world tasks. Twenty-four of 30 patients remained implanted with functioning Argus II Systems at 5 years after implantation. Only 1 additional serious adverse event was experienced after the 3-year time point. Patients performed significantly better with the Argus II on than off on all visual function tests and functional vision tasks. The 5-year results of the Argus II trial support the long-term safety profile and benefit of the Argus II System for patients blind as a result of RP. The Argus II is the first and only retinal implant to have market approval in the European Economic Area, the United States, and Canada. Copyright © 2016 American Academy of Ophthalmology. Published by Elsevier Inc. All rights reserved.

  11. A Wind Farm Electrical Systems Evaluation with EeFarm-II

    NARCIS (Netherlands)

    Pierik, J.; Axelsson, U.; Eriksson, E.; Salomonsson, D.; Bauer, P.; Czech, B.

    2010-01-01

    EeFarm-II is used to evaluate 13 different electrical systems for a 200 MW wind farm with a 100 km connection to shore. The evaluation is based on component manufacturer data of 2009. AC systems are compared to systems with DC connections inside the wind farm and DC connection to shore. Two options

  12. One-step deterministic multipartite entanglement purification with linear optics

    Energy Technology Data Exchange (ETDEWEB)

    Sheng, Yu-Bo [Department of Physics, Tsinghua University, Beijing 100084 (China); Long, Gui Lu, E-mail: gllong@tsinghua.edu.cn [Department of Physics, Tsinghua University, Beijing 100084 (China); Center for Atomic and Molecular NanoSciences, Tsinghua University, Beijing 100084 (China); Key Laboratory for Quantum Information and Measurements, Beijing 100084 (China); Deng, Fu-Guo [Department of Physics, Applied Optics Beijing Area Major Laboratory, Beijing Normal University, Beijing 100875 (China)

    2012-01-09

    We present a one-step deterministic multipartite entanglement purification scheme for an N-photon system in a Greenberger–Horne–Zeilinger state with linear optical elements. The parties in quantum communication can in principle obtain a maximally entangled state from each N-photon system with a success probability of 100%. That is, it does not consume the less-entangled photon systems largely, which is far different from other multipartite entanglement purification schemes. This feature maybe make this scheme more feasible in practical applications. -- Highlights: ► We proposed a deterministic entanglement purification scheme for GHZ states. ► The scheme uses only linear optical elements and has a success probability of 100%. ► The scheme gives a purified GHZ state in just one-step.

  13. Computational Models for Nonlinear Aeroelastic Systems, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — Clear Science Corp. and Duke University propose to develop and demonstrate new and efficient computational methods of modeling nonlinear aeroelastic systems. The...

  14. Reliable High Performance Processing System (RHPPS), Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — NASA's exploration, science, and space operations systems are critically dependent on the hardware technologies used in their implementation. Specifically, the...

  15. Nonhazardous Urine Pretreatment Method for Future Exploration Systems, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — A nonhazardous urine pretreatment system prototype is proposed that will stabilize urine against biological growth or chemical instabilities without using hazardous...

  16. Fiber Optic Temperature Sensors for Thermal Protection Systems, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — In Phase 1, Intelligent Fiber Optic Systems Corporation (IFOS), in collaboration with North Carolina State University, successfully demonstrated a Fiber Bragg...

  17. A Gimbal-Stabilized Compact Hyperspectral Imaging System, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — The Gimbal-stabilized Compact Hyperspectral Imaging System (GCHIS) fully integrates multi-sensor spectral imaging, stereovision, GPS and inertial measurement,...

  18. Intelligent Flamefinder Detection and Alert System (IFDAS), Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — Current hydrogen flame detection systems exhibit shortcomings ranging from limited detection range, to localization inaccuracy, limited sensitivity, false alarms,...

  19. Software Defined Common Processing System (SDCPS), Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — Coherent Logix, Incorporated (CLX) proposes the development of a Software Defined Common Processing System (SDCPS) that leverages the inherent advantages of an...

  20. Model Updating Nonlinear System Identification Toolbox, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — ZONA Technology (ZONA) proposes to develop an enhanced model updating nonlinear system identification (MUNSID) methodology that utilizes flight data with...

  1. On the Importance of Purification of Sodium Polystyrene Sulfonate

    OpenAIRE

    Sen, Akhil K.; Roy, Sandip; Juvekar, Vinay A.

    2012-01-01

    Ion exchange is commonly employed for purification of sodium polystyrene sulfonate (NaPSS), a molecule widely used as a model polyelectrolyte. However, the present work demonstrates that the ion exchange process itself may introduce some extraneous species into NaPSS samples by two possible mechanisms: (i) chemical transformation of polystyrene sulfonic acid (HPSS), a relatively unstable intermediate formed during ion exchange and (ii) release of small amount of “condensed” acid from cationic...

  2. A Wind Farm Electrical Systems Evaluation with EeFarm-II

    Directory of Open Access Journals (Sweden)

    Jan Pierik

    2010-03-01

    Full Text Available EeFarm-II is used to evaluate 13 different electrical systems for a 200 MW wind farm with a 100 km connection to shore. The evaluation is based on component manufacturer data of 2009. AC systems are compared to systems with DC connections inside the wind farm and DC connection to shore. Two options have the best performance for this wind farm size and distance: the AC system and the system with a DC connection to shore. EeFarm-II is a user friendly computer program for wind farm electrical and economic evaluation. It has been built as a Simulink Library in the graphical interface of Matlab-Simulink. EeFarm-II contains models of wind turbines, generators, transformers, AC cables, inductors, nodes, splitters, PWM converters, thyristor converters, DC cables, choppers and statcoms.

  3. Development of FBR cycle data base system (II)

    International Nuclear Information System (INIS)

    Kubota, Sadae; Ohtaki, Akira; Hirao, Kazuhiro

    2003-05-01

    In the 'Feasibility Study on Commercialized FBR Cycle Systems (F/S)', scenario evaluations, cost-benefit evaluations and system characteristic evaluations to show the significance of the FBR cycle system introduction concretely are performed while design studies for FBR plants, reprocessing systems and fabrication systems are conducted. In these evaluations, future society of various conditions and situation is assumed, and investigation and analysis about needs and social effects of FBR cycle are carried out. In this study, promising FBR cycle concepts are suggested by taking information such as domestic and foreign policies and bills, an economic prediction, a supply and demand prediction of resources, a project of technology development into consideration in addition to system design information. The development of the FBR Cycle Database which this report introduced started in 1999 fiscal year to enable managed unitarity and searched reference information to use for the above scenario evaluations, cost-benefit evaluations and system characteristic evaluations. In 2000 fiscal year, its prototype was made and used tentatively, and we extracted the problems in operation and functions from that, and, in 2001 fiscal year, the entry system and the search system using the Web page were made in order to solve problems of the prototype, and started use in our group. Moreover, in 2002 fiscal year, we expanded and improved the search system and promoted the efficiency of management work, and use in JNC through intranet of the database was started. In addition, as a result of having made the entry of about 350 data in 2002 fiscal year, the collected number of the database reaches about 7,250 by the end of March, 2003. We are to continue the entry of related information of various evaluations in F/S phase 2 from now on. In addition, we are to examine improvement of convenience of the search system and cooperation with the economy database. (author)

  4. A study on the front-end VME system of BEPC II

    International Nuclear Information System (INIS)

    Wang Chunhong

    2004-01-01

    The front-end VME system is not only the heart of the control system, but also a real-time system. This paper describes the component of the front-end VME (Versa Module Eurocard) system including control computer and some related I/O modules. Particularly, the authors present a best solution for the problems about Vx-Works kernel and BSP running on MVME5100. This is a fundamental setup of the BEPC II control system. (author)

  5. A/C Interface: Expert Systems: Part II.

    Science.gov (United States)

    Dessy, Raymond E., Ed.

    1984-01-01

    Discusses working implementations of artificial intelligence systems for chemical laboratory applications. They include expert systems for liquid chromatography, spectral analysis, instrument control of a totally computerized triple-quadrupole mass spectrometer, and the determination of the mineral constituents of a rock sample given the powder…

  6. Five-year safety and performance results from the Argus II Retinal Prosthesis System clinical trial

    Science.gov (United States)

    da Cruz, Lyndon; Dorn, Jessy D.; Humayun, Mark S.; Dagnelie, Gislin; Handa, James; Barale, Pierre-Olivier; Sahel, José-Alain; Stanga, Paulo E.; Hafezi, Farhad; Safran, Avinoam B.; Salzmann, Joel; Santos, Arturo; Birch, David; Spencer, Rand; Cideciyan, Artur V.; de Juan, Eugene; Duncan, Jacque L.; Eliott, Dean; Fawzi, Amani; Olmos de Koo, Lisa C.; Ho, Allen C.; Brown, Gary; Haller, Julia; Regillo, Carl; Del Priore, Lucian V.; Arditi, Aries; Greenberg, Robert J.

    2016-01-01

    Purpose The Argus® II Retinal Prosthesis System (Second Sight Medical Products, Inc., Sylmar, CA) was developed to restore some vision to patients blind from retinitis pigmentosa (RP) or outer retinal degeneration. A clinical trial was initiated in 2006 to study the long-term safety and efficacy of the Argus II System in patients with bare or no light perception due to end-stage RP. Design The study is a prospective, multicenter, single-arm, clinical trial. Within-patient controls included the non-implanted fellow eye and patients' native residual vision compared to their vision when using the System. Subjects There were 30 subjects in 10 centers in the U.S. and Europe. Methods The worse-seeing eye of blind patients was implanted with the Argus II System. Patients wore glasses mounted with a small camera and a video processor that converted images into stimulation patterns sent to the electrode array on the retina. Main Outcome Measures The primary outcome measures were safety (the number, seriousness, and relatedness of adverse events) and visual function, as measured by three computer-based, objective tests. Secondary measures included functional vision performance on objectively-scored real-world tasks. Results Twenty-four out of 30 patients remained implanted with functioning Argus II Systems at 5 years post-implant. Only one additional serious adverse event was experienced since the 3-year time point. Patients performed significantly better with the System ON than OFF on all visual function tests and functional vision tasks. Conclusions The five-year results of the Argus II trial support the long-term safety profile and benefit of the Argus II System for patients blind from RP. The Argus II is the first and only retinal implant to have market approval in the European Economic Area, the United States, and Canada. PMID:27453256

  7. Nuclear instrumentation system operating experience and nuclear instrument testing in the EBR-II

    International Nuclear Information System (INIS)

    Yingling, G.E.; Curran, R.N.

    1980-01-01

    In March of 1972 three wide range nuclear channels were purchased from Gulf Atomics Corporation and installed in EBR-II as a test. The three channels were operated as a test until April 1975 when they became a permanent part of the reactor shutdown system. Also described are the activities involved in evaluating and qualifying neutron detectors for LMFBR applications. Included are descriptions of the ANL Components Technology Division Test Program and the EBR-II Nuclear Instrument Test Facilities (NITF) used for the in-reactor testing and a summary of program test results from EBR-II

  8. Zn(II, Mn(II and Sr(II Behavior in a Natural Carbonate Reservoir System. Part I: Impact of Salinity, Initial pH and Initial Zn(II Concentration in Atmospheric Conditions

    Directory of Open Access Journals (Sweden)

    Auffray B.

    2016-07-01

    Full Text Available The sorption of inorganic elements on carbonate minerals is well known in strictly controlled conditions which limit the impact of other phenomena such as dissolution and/or precipitation. In this study, we evidence the behavior of Zn(II (initially in solution and two trace elements, Mn(II and Sr(II (released by carbonate dissolution in the context of a leakage from a CO2 storage site. The initial pH chosen are either equal to the pH of the water-CO2 equilibrium (~ 2.98 or equal to the pH of the water-CO2-calcite system (~ 4.8 in CO2 storage conditions. From this initial influx of liquid, saturated or not with respect to calcite, the batch experiments evolve freely to their equilibrium, as it would occur in a natural context after a perturbation. The batch experiments are carried out on two natural carbonates (from Lavoux and St-Emilion with PCO2 = 10−3.5 bar, with different initial conditions ([Zn(II]i from 10−4 to 10−6 M, either with pure water or 100 g/L NaCl brine. The equilibrium regarding calcite dissolution is confirmed in all experiments, while the zinc sorption evidenced does not always correspond to the two-step mechanism described in the literature. A preferential sorption of about 10% of the concentration is evidenced for Mn(II in aqueous experiments, while Sr(II is more sorbed in saline conditions. This study also shows that this preferential sorption, depending on the salinity, is independent of the natural carbonate considered. Then, the simulations carried out with PHREEQC show that experiments and simulations match well concerning the equilibrium of dissolution and the sole zinc sorption, with log KZn(II ~ 2 in pure water and close to 4 in high salinity conditions. When the simulations were possible, the log K values for Mn(II and Sr(II were much different from those in the literature obtained by sorption in controlled conditions. It is shown that a new conceptual model regarding multiple Trace Elements (TE sorption is

  9. Predictive value of SAPS II and APACHE II scoring systems for patient outcome in a medical intensive care unit

    Directory of Open Access Journals (Sweden)

    Amina Godinjak

    2016-11-01

    Full Text Available Objective. The aim is to determine SAPS II and APACHE II scores in medical intensive care unit (MICU patients, to compare them for prediction of patient outcome, and to compare with actual hospital mortality rates for different subgroups of patients. Methods. One hundred and seventy-four patients were included in this analysis over a oneyear period in the MICU, Clinical Center, University of Sarajevo. The following patient data were obtained: demographics, admission diagnosis, SAPS II, APACHE II scores and final outcome. Results. Out of 174 patients, 70 patients (40.2% died. Mean SAPS II and APACHE II scores in all patients were 48.4±17.0 and 21.6±10.3 respectively, and they were significantly different between survivors and non-survivors. SAPS II >50.5 and APACHE II >27.5 can predict the risk of mortality in these patients. There was no statistically significant difference in the clinical values of SAPS II vs APACHE II (p=0.501. A statistically significant positive correlation was established between the values of SAPS II and APACHE II (r=0.708; p=0.001. Patients with an admission diagnosis of sepsis/septic shock had the highest values of both SAPS II and APACHE II scores, and also the highest hospital mortality rate of 55.1%. Conclusion. Both APACHE II and SAPS II had an excellent ability to discriminate between survivors and non-survivors. There was no significant difference in the clinical values of SAPS II and APACHE II. A positive correlation was established between them. Sepsis/septic shock patients had the highest predicted and observed hospital mortality rate.

  10. Development of a safety and regulation systems simulation program II

    International Nuclear Information System (INIS)

    1985-05-01

    This report describes the development of a safety and regulation systems simulation program under contract to the Atomic Energy Control Board of Canada. A systems logic interaction simulation (SLISIM) program was developed for the AECB's HP-1000 computer which operates in the interactive simulation (INSIM) program environment. The SLISIM program simulates the spatial neutron dynamics, the regulation of the reactor power and in this version the CANDU-PHW 600 MW(e) computerized shutdown systems' trip parameters. The modular concept and interactive capability of the INSIM environment provides the user with considerable flexibility of the setup and control of the simulation

  11. Tritium system design for the mirror reactors FPD-I, FPD-II, and FPD-III

    International Nuclear Information System (INIS)

    Finn, P.A.

    1985-01-01

    The tritium system design for the Fusion Power Demonstration Reactor (FPD-I, II, and III) is described. The device operates at 25% availability. For FPD-II, an engineering mode using tritium neutral beams is part of the design

  12. Juvenile idiopathic arthritis (JIA): a screening study to measure class II skeletal pattern, TMJ PDS and use of systemic corticosteroids.

    LENUS (Irish Health Repository)

    Mandall, Nicky A

    2010-03-01

    To screen patients with oligoarticular and polyarticular forms of Juvenile Idiopathic Arthritis (JIA) to determine (i) the severity of their class II skeletal pattern; (ii) temporomandibular joint signs and symptoms and (iii) use of systemic corticosteroids.

  13. Sample problems for the novice user of the AMPX-II system

    International Nuclear Information System (INIS)

    Ford, W.E. III; Roussin, R.W.; Petrie, L.M.; Diggs, B.R.; Comolander, H.E.

    1979-01-01

    Contents of the IBM version of the APMX system distributed by the Radiation Shielding Information Center (APMX-II) are described. Sample problems which demonstrate the procedure for implementing AMPX-II modules to generate point cross sections; generate multigroup neutron, photon production, and photon interaction cross sections for various transport codes; collapse multigroup cross sections; check, edit, and punch multigroup cross sections; and execute a one-dimensional discrete ordinates transport calculation are detailed. 25 figures, 9 tables

  14. Analysis of beam feedback loops of RF acceleration system at TARN II

    International Nuclear Information System (INIS)

    Katayama, Takeshi.

    1992-08-01

    Two beam-feedback-loops are prepared for the frequency control of RF acceleration system at cooler-synchrotron TARN II. One is the phase-loop and the other the radial-position-loop. In the present paper, the effects of these loops on the beam dynamics in the synchrotron are studied on the basis of Laplace transformation approach as well as the numerical values for the synchrotron acceleration at TARN II. (author)

  15. Flexible and Safe Control of Mobile Surface Systems, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — The primary innovation of this work is a novel approach for flexible and safe control of highly capable mobile surface systems, such as long-duration science rovers,...

  16. Extreme Temperature Motor and Drill System, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — In response to the need for motors, actuators and drilling systems that can operate in the harsh venusian environment for extended periods of time, on the order of...

  17. Reactive In-flight Multisensor Security System (RIMSS), Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — The need for in-flight event detection and monitoring systems is clear. To address this and other safety and security needs, IEM proposed the Reactive In-flight...

  18. Windhover Unmanned Aircraft Systems (UAS) Software Ecosystem, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — The safety of Unmanned Aircraft Systems (UAS) flights is currently the responsibility of the pilot who is required to keep the vehicle within their line of sight...

  19. Sample Management System for Heavy Ion Irradiation, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — A robotic sample management device and system for the exposure of biological and material specimens to heavy ion beams of the NASA Space Radiation Laboratory (NSRL)...

  20. Defining Handling Qualities of Unmanned Aerial Systems, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — Unmanned Air Systems (UAS) are no longer coming, they are here, and operators from first responders to commercial operators are demanding access to the National...

  1. Validation Tools and Methods for Diagnostic Systems, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — The potential benefits of advanced algorithms for diagnostics and prognostics, inner-loop control, and other flight critical systems have been demonstrated in a...

  2. Carbonaceous Asteroid Volatile Recovery (CAVoR) system, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — The Carbonaceous Asteroid Volatile Recovery (CAVoR) system produces water and hydrogen-rich syngas for propellant production, life support consumables, and...

  3. Aircraft Nodal Data Acquisition System (ANDAS), Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — Development of an Aircraft Nodal Data Acquisition System (ANDAS) based upon the short haul Zigbee networking standard is proposed. It employs a very thin (135 um)...

  4. Novel Real-Time Flight Envelope Monitoring System, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — The proposed innovation is an aircraft flight envelope monitoring system that will provide real-time in-cockpit estimations of aircraft flight envelope boundaries....

  5. NDE for Ablative Thermal Protection Systems, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — This program addresses the need for non-destructive evaluation (NDE) methods for quality assessment and defect evaluation of thermal protection systems (TPS). Novel...

  6. Open System of Agile Ground Stations, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — The Phase I effort demonstrated, through actual development and tests with a spacecraft system, the technical and programmatic feasibility of developing, within the...

  7. Silver Ion Biocide Delivery System for Water Disinfection, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — U.S. space exploration missions have long considered returning to the Moon and exploration of Mars that challenge life support systems. For these long duration...

  8. Advanced On Board Inert Gas Generation System (OBBIGS), Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — Valcor Engineering Corporation proposes to develop an advanced On Board Inert Gas Generation System, OBIGGS, for aircraft fuel tank inerting to prevent hazardous...

  9. Wasp-II application to Brazilian Electric System

    International Nuclear Information System (INIS)

    Sakamoto, L.H.

    1980-01-01

    In the next years, the expansion of the Brazilian Electric System will occur in two large sub-systems struture: South-Southeast and North-Northeast. The interconnection between these systems is uncertain due to long distance between then. Hence, it seems more reasonable to study them separately and the South-Southeast System was chosen because it is more important from the economic point of view. Some information concerning input data used in this study was estimated, therefore a thorough review of them must be done for more for more conclusive results. Hydro, coal and nuclear power plants were considered as candidates for an expansion plan starting in 1987 to 2000. (author) [pt

  10. WASP-II Aplication to Brazilian electric system

    International Nuclear Information System (INIS)

    Sakamoto, L.H.

    1980-12-01

    In the next years, the expansion of the Brazilian Electric System will occur in a two large sub-systems structure: South-Southeast and North-Northeast. The interconnection between these systems are uncertain due the long distance between them. Hence, it seems more reasonable to study separately and the South-Southeast System was chosen because it is more important from the economic point of view. Some information concerning input data used in this study was estimated, therefore a thorough review of them must be done for more conclusive results. Hydro, coal and nuclear power plants were considered as candidates for an expansion plan starting in 1987 up to 2000. (Author) [pt

  11. Extremelly High Bandwidth Rad Hard Data Acquisition System, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — Analog-to-digital converters (ADCs) are the key components for digitizing high-speed analog data in modern data acquisition systems, which is a critical part of...

  12. Sensor Area Network for Integrated Systems Health Management, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — The term Integrated Systems Health Management (ISHM) is used to describe a capability that focuses on determining the condition (health) of every element in a...

  13. Miniaturized Airborne Imaging Central Server System, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — The innovation is a miniaturized airborne imaging central server system (MAICSS). MAICSS is designed as a high-performance computer-based electronic backend that...

  14. Multi-Purpose X-ray System, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — The proposed Multi-Purpose X-ray Source and System (MPXS) can be used on flight missions, space stations, planetary excursions and planetary or asteroid bases, to...

  15. Stability of position control system in JIPP T-II

    International Nuclear Information System (INIS)

    Sakurai, Keiichi; Tanahashi, Shygo

    1980-01-01

    Computations and experiments on the stability of a feedback control system for maintaining a plasma column in equilibrium are described. The time response of the displacement of the plasma to the desired position is examined by solving the equation of motion of the plasma column. We show that the stability of the feedback control system is improved by using an additional term which represents the shift velocity of the plasma column. (author)

  16. PWL approximation of nonlinear dynamical systems, part II: identification issues

    International Nuclear Information System (INIS)

    De Feo, O; Storace, M

    2005-01-01

    This paper and its companion address the problem of the approximation/identification of nonlinear dynamical systems depending on parameters, with a view to their circuit implementation. The proposed method is based on a piecewise-linear approximation technique. In particular, this paper describes a black-box identification method based on state space reconstruction and PWL approximation, and applies it to some particularly significant dynamical systems (two topological normal forms and the Colpitts oscillator)

  17. Rapid purification of recombinant histones.

    Science.gov (United States)

    Klinker, Henrike; Haas, Caroline; Harrer, Nadine; Becker, Peter B; Mueller-Planitz, Felix

    2014-01-01

    The development of methods to assemble nucleosomes from recombinant histones decades ago has transformed chromatin research. Nevertheless, nucleosome reconstitution remains time consuming to this day, not least because the four individual histones must be purified first. Here, we present a streamlined purification protocol of recombinant histones from bacteria. We termed this method "rapid histone purification" (RHP) as it circumvents isolation of inclusion bodies and thereby cuts out the most time-consuming step of traditional purification protocols. Instead of inclusion body isolation, whole cell extracts are prepared under strongly denaturing conditions that directly solubilize inclusion bodies. By ion exchange chromatography, the histones are purified from the extracts. The protocol has been successfully applied to all four canonical Drosophila and human histones. RHP histones and histones that were purified from isolated inclusion bodies had similar purities. The different purification strategies also did not impact the quality of octamers reconstituted from these histones. We expect that the RHP protocol can be readily applied to the purification of canonical histones from other species as well as the numerous histone variants.

  18. The evaluation of CRIB II scoring system in predicting mortality in preterm newborns

    Directory of Open Access Journals (Sweden)

    Homa Babaei

    2015-02-01

    Full Text Available Background: The survival rate of premature newborns depends on gestational age, birth weight and condition when they are hospitalized. Different scoring systems to predict mortality in newborns has been designed. The purpose of this study was to evaluate value of CRIB II scoring system in predicting mortality rate of infants with birth weights less than 1500 grams. Material and Methods: In this 8 month cross - sectional study (September 2010 to April 2010 which was conducted in the NICU of Imam Reza hospital in Kermanshah, preterm newborns with birth weight less than 1500 gr and gestational age less than 32 weeks who were admitted within 12 hours after birth in the NICU ,were evaluated based on CRIB II scoring system . Results: 50 neonates out of 1360 (36.8% survived and 86 neonates(63.2% died. Average CRIB II score in newborn survived was 5.8±2.9 and in infants died was 9.8±2.9 (p <0.0001. Based on the AUC, the CRIB II score could predict about 0.85 (CI: 0.77-0.92 of mortality. Also based on the ROC curve cut-off point for scoring CRIB II, was 6.5. Conclusion: Our study showed that CRIB II has a high value( about %85 in predicting mortality in newborns with birth weights less than 1500 grams.

  19. The nuclear data collecting system designed with ARM and μC/OS-II

    International Nuclear Information System (INIS)

    Wang Chunsheng; Ma Yingjie; Han Feng

    2008-01-01

    Introduce a kind of nuclear data collecting system regarding ARM-μC/OS-II as the platform, gathering the GPS receiver in it. It was detailed to expatiated the key techniques of the multi-channel pulse amplitude analyzer, the interface design of LPC2148, a controller in ARM, and how to expand the RTOS and design applications on μC/OS-II. This system can communicate with the GPS-OEM module by the UART interface, collecting the GPS information synchronously as well as nuclear data. And then save and display them or pass them to the host computer by the USB interface. The embedded and Real-Time system, μC/OS-II build up the real-time and stability of the system and advance the integration. (authors)

  20. Research on advanced system safety assessment procedures (II)

    International Nuclear Information System (INIS)

    Suzuki, Kazuhiko; Shimada, Yukiyasu

    1999-03-01

    HAZOP (Hazard and operability study) is a systematic technique, which requires the involvement of an experienced, interdisciplinary team of engineers, to identify hazards or operability problems throughout an entire facility by brainstorming. Though HAZOP is recognized as the useful safety assessment method, it requires a labor-intensive and time-consuming process. So recently computer-aided HAZOP has been proposed. The research report in 1998 (PNC PJ1612 98-001) presented prototype system, which carries out HAZOP and FT synthesis, by making use of proposed method. Relationships between states of input and output variables, internal and external events of each component are represented using decision tables, and the system is implemented by C++. In this study, the causalities of plant component malfunctions are described as component malfunction basic model and are stored in the computer. Thus, we have developed safety evaluation support system by considering the fault propagation path. Component malfunction basic model is made based on the information on the causalities between the abnormal state and each malfunction in components. This component malfunction basic model provides the common frame to describe abnormal situation in components. By using this basic model, not only state malfunction of component but also the consequence to external circumstance is assessed. G2, which is an excellent object-oriented developer tool in GUI (Graphical User Interface), is used as a tool for developing the system. By using the graphical editor in the system, the user can carry out HAZOP easily. We have applied this system to the Nuclear Reprocessing Facilities to demonstrate the utilities of developing system. (author)

  1. Automated multi-dimensional purification of tagged proteins.

    Science.gov (United States)

    Sigrell, Jill A; Eklund, Pär; Galin, Markus; Hedkvist, Lotta; Liljedahl, Pia; Johansson, Christine Markeland; Pless, Thomas; Torstenson, Karin

    2003-01-01

    The capacity for high throughput purification (HTP) is essential in fields such as structural genomics where large numbers of protein samples are routinely characterized in, for example, studies of structural determination, functionality and drug development. Proteins required for such analysis must be pure and homogenous and available in relatively large amounts. AKTA 3D system is a powerful automated protein purification system, which minimizes preparation, run-time and repetitive manual tasks. It has the capacity to purify up to 6 different His6- or GST-tagged proteins per day and can produce 1-50 mg protein per run at >90% purity. The success of automated protein purification increases with careful experimental planning. Protocol, columns and buffers need to be chosen with the final application area for the purified protein in mind.

  2. Modeling multibody systems with uncertainties. Part II: Numerical applications

    International Nuclear Information System (INIS)

    Sandu, Corina; Sandu, Adrian; Ahmadian, Mehdi

    2006-01-01

    This study applies generalized polynomial chaos theory to model complex nonlinear multibody dynamic systems operating in the presence of parametric and external uncertainty. Theoretical and computational aspects of this methodology are discussed in the companion paper 'Modeling Multibody Dynamic Systems With Uncertainties. Part I: Theoretical and Computational Aspects .In this paper we illustrate the methodology on selected test cases. The combined effects of parametric and forcing uncertainties are studied for a quarter car model. The uncertainty distributions in the system response in both time and frequency domains are validated against Monte-Carlo simulations. Results indicate that polynomial chaos is more efficient than Monte Carlo and more accurate than statistical linearization. The results of the direct collocation approach are similar to the ones obtained with the Galerkin approach. A stochastic terrain model is constructed using a truncated Karhunen-Loeve expansion. The application of polynomial chaos to differential-algebraic systems is illustrated using the constrained pendulum problem. Limitations of the polynomial chaos approach are studied on two different test problems, one with multiple attractor points, and the second with a chaotic evolution and a nonlinear attractor set. The overall conclusion is that, despite its limitations, generalized polynomial chaos is a powerful approach for the simulation of multibody dynamic systems with uncertainties

  3. Modeling multibody systems with uncertainties. Part II: Numerical applications

    Energy Technology Data Exchange (ETDEWEB)

    Sandu, Corina, E-mail: csandu@vt.edu; Sandu, Adrian; Ahmadian, Mehdi [Virginia Polytechnic Institute and State University, Mechanical Engineering Department (United States)

    2006-04-15

    This study applies generalized polynomial chaos theory to model complex nonlinear multibody dynamic systems operating in the presence of parametric and external uncertainty. Theoretical and computational aspects of this methodology are discussed in the companion paper 'Modeling Multibody Dynamic Systems With Uncertainties. Part I: Theoretical and Computational Aspects .In this paper we illustrate the methodology on selected test cases. The combined effects of parametric and forcing uncertainties are studied for a quarter car model. The uncertainty distributions in the system response in both time and frequency domains are validated against Monte-Carlo simulations. Results indicate that polynomial chaos is more efficient than Monte Carlo and more accurate than statistical linearization. The results of the direct collocation approach are similar to the ones obtained with the Galerkin approach. A stochastic terrain model is constructed using a truncated Karhunen-Loeve expansion. The application of polynomial chaos to differential-algebraic systems is illustrated using the constrained pendulum problem. Limitations of the polynomial chaos approach are studied on two different test problems, one with multiple attractor points, and the second with a chaotic evolution and a nonlinear attractor set. The overall conclusion is that, despite its limitations, generalized polynomial chaos is a powerful approach for the simulation of multibody dynamic systems with uncertainties.

  4. Computerized Operator Support System – Phase II Development

    Energy Technology Data Exchange (ETDEWEB)

    Ulrich, Thomas A.; Boring, Ronald L.; Lew, Roger T.; Thomas, Kenneth D.

    2015-02-01

    A computerized operator support system (COSS) prototype for nuclear control room process control is proposed and discussed. The COSS aids operators in addressing rapid plant upsets that would otherwise result in the shutdown of the power plant and interrupt electrical power generation, representing significant costs to the owning utility. In its current stage of development the prototype demonstrates four advanced functions operators can use to more efficiently monitor and control the plant. These advanced functions consist of: (1) a synthesized and intuitive high level overview display of system components and interrelations, (2) an enthalpy-based mathematical chemical and volume control system (CVCS) model to detect and diagnose component failures, (3) recommended strategies to mitigate component failure effects and return the plant back to pre-fault status, and (4) computer-based procedures to walk the operator through the recommended mitigation actions. The COSS was demonstrated to a group of operators and their feedback was collected. The operators responded positively to the COSS capabilities and features and indicated the system would be an effective operator aid. The operators also suggested several additional features and capabilities for the next iteration of development. Future versions of the COSS prototype will include additional plant systems, flexible computer-based procedure presentation formats, and support for simultaneous component fault diagnosis and dual fault synergistic mitigation action strategies to more efficiently arrest any plant upsets.

  5. THE ELM SURVEY. II. TWELVE BINARY WHITE DWARF MERGER SYSTEMS

    International Nuclear Information System (INIS)

    Kilic, Mukremin; Brown, Warren R.; Kenyon, S. J.; Prieto, Carlos Allende; Agueeros, M. A.; Heinke, Craig

    2011-01-01

    We describe new radial velocity and X-ray observations of extremely low-mass white dwarfs (ELM WDs, ∼0.2 M sun ) in the Sloan Digital Sky Survey Data Release 4 and the MMT Hypervelocity Star survey. We identify four new short period binaries, including two merger systems. These observations bring the total number of short period binary systems identified in our survey to 20. No main-sequence or neutron star companions are visible in the available optical photometry, radio, and X-ray data. Thus, the companions are most likely WDs. Twelve of these systems will merge within a Hubble time due to gravitational wave radiation. We have now tripled the number of known merging WD systems. We discuss the characteristics of this merger sample and potential links to underluminous supernovae, extreme helium stars, AM CVn systems, and other merger products. We provide new observational tests of the WD mass-period distribution and cooling models for ELM WDs. We also find evidence for a new formation channel for single low-mass WDs through binary mergers of two lower mass objects.

  6. Fiscal 2000 report on entrusted follow-up project for research cooperation on simplified industrial wastewater purification system; 2000 nendo sangyo haisui nado no kan'i joka system ni kansuru kenkyu kyoryoku follow up gyomu hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    The Research Cooperation Project on Simplified Industrial Wastewater Purification System carried out in cooperation with Indonesia and Malaysia was terminated in fiscal 1998. A follow-up check was conducted for spreading the fruits of the project. In concrete terms, for the study of problems to solve for the dissemination across Malaysia of the results of the project implemented in that country, technical guidance was provided relative to the study of operation performed by the counterpart operating their own pilot plant and the like. International Meeting for Technical Exchange was participated in, convened in Thailand in the period March 6-8, 2001. The contents of research activities included the two fields of (1) the selection of coagulants and (2) the study of pilot plant operation. In field (1), PAC (poly-aluminum chloride) and aluminum sulfate were compared with each other in terms of performance and cost in case they were used as coagulants, and then it was made clear that PAC was superior. In field (2), operation was studied using UASB (upflow anaerobic sludge blanket) type facilities of Southern Acid Plant. (NEDO)

  7. Fiscal 2000 report on entrusted follow-up project for research cooperation on simplified industrial wastewater purification system; 2000 nendo sangyo haisui nado no kan'i joka system ni kansuru kenkyu kyoryoku follow up gyomu hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    The Research Cooperation Project on Simplified Industrial Wastewater Purification System carried out in cooperation with Indonesia and Malaysia was terminated in fiscal 1998. A follow-up check was conducted for spreading the fruits of the project. In concrete terms, for the study of problems to solve for the dissemination across Malaysia of the results of the project implemented in that country, technical guidance was provided relative to the study of operation performed by the counterpart operating their own pilot plant and the like. International Meeting for Technical Exchange was participated in, convened in Thailand in the period March 6-8, 2001. The contents of research activities included the two fields of (1) the selection of coagulants and (2) the study of pilot plant operation. In field (1), PAC (poly-aluminum chloride) and aluminum sulfate were compared with each other in terms of performance and cost in case they were used as coagulants, and then it was made clear that PAC was superior. In field (2), operation was studied using UASB (upflow anaerobic sludge blanket) type facilities of Southern Acid Plant. (NEDO)

  8. The ATLAS Data Flow System for LHC Run II

    CERN Document Server

    AUTHOR|(INSPIRE)INSPIRE-00305920; The ATLAS collaboration

    2016-01-01

    After its first shutdown, the LHC will provide pp collisions with increased luminosity and energy. In the ATLAS experiment, the Trigger and Data Acquisition (TDAQ) system has been upgraded to deal with the increased event rates. The Data Flow (DF) element of the TDAQ is a distributed hardware and software system responsible for buffering and transporting event data from the readout system to the High Level Trigger (HLT) and to the event storage. The DF has been reshaped in order to profit from the technological progress and to maximize the flexibility and efficiency of the data selection process. The updated DF is radically different from the previous implementation both in terms of architecture and expected performance. The pre-existing two level software filtering, known as L2 and the Event Filter, and the Event Building are now merged into a single process, performing incremental data collection and analysis. This design has many advantages, among which are: the radical simplification of the architecture, ...

  9. Ultrasound Vector Flow Imaging: Part II: Parallel Systems

    DEFF Research Database (Denmark)

    Jensen, Jørgen Arendt; Nikolov, Svetoslav Ivanov; Yu, Alfred C. H.

    2016-01-01

    The paper gives a review of the current state-of-theart in ultrasound parallel acquisition systems for flow imaging using spherical and plane waves emissions. The imaging methods are explained along with the advantages of using these very fast and sensitive velocity estimators. These experimental...... ultrasound imaging for studying brain function in animals. The paper explains the underlying acquisition and estimation methods for fast 2-D and 3-D velocity imaging and gives a number of examples. Future challenges and the potentials of parallel acquisition systems for flow imaging are also discussed....

  10. An impulsive predator-prey system with modified Leslie-Gower and Holling type II schemes

    International Nuclear Information System (INIS)

    Guo Hongjian; Song Xinyu

    2008-01-01

    An impulsive predator-prey system with modified Leslie-Gower and Holling-type II schemes is presented. By using the Floquet theory of impulsive equation and small amplitude perturbation method, the globally asymptotical stability of prey-free positive periodic solution and the permanence of system are discussed. The corresponding threshold conditions are obtained respectively. Finally, numerical simulations are given

  11. The ATLAS Trigger System: Ready for Run II

    CERN Document Server

    Czodrowski, Patrick; The ATLAS collaboration

    2015-01-01

    The ATLAS trigger system has been used successfully for data collection in the 2009-2013 Run 1 operation cycle of the CERN Large Hadron Collider (LHC) at center-of-mass energies of up to 8 TeV. With the restart of the LHC for the new Run 2 data-taking period at 13 TeV, the trigger rates are expected to rise by approximately a factor of 5. The trigger system consists of a hardware-based first level (L1) and a software-based high-level trigger (HLT) that reduces the event rate from the design bunch-crossing rate of 40 MHz to an average recording rate of ~ 1kHz. This presentation will give an overview of the upgrades to the ATLAS trigger system that have been implemented during the LHC shutdown period in order to deal with the increased trigger rates while efficiently selecting the physics processes of interest. These upgrades include changes to the L1 calorimeter trigger, the introduction of a new L1 topological trigger module, improvements in the L1 muon system, and the merging of the previously two-level HLT ...

  12. Heat transfer calculations on the KNK II emergency cooling system

    International Nuclear Information System (INIS)

    Vossebrecker, H.; Groenefeld, G.

    1976-12-01

    The Licensing Authority had demanded that in case of the change of the KNK thermal core into a fast core the decay heat removal system must be improved by a diverse and spatially separated emergency cooling system. In order to meet this requirement an existing nitrogen system of the facility is extended in such a manner that the decay heat will be removed by a nitrogen flow passing through the gap between reactor vessel and guard vessel. The heat transport from the core to the vessel is accomplished by natural convection flow rates which are generated by density differences between the hot core subassemblies, the reflector subassemblies and other passages between the upper and the lower plenum. The calculations show that the maximum temperatures in the core do not reach the sodium boiling-point. The maximum vessel temperature is 673 deg. C. In this report the function of the emergency cooling system and the methods of calculation are described, the input data and the results are stated and it is shown that the calculated temperatures are conservative [de

  13. Health Occupations Module. The Skeletal System--II.

    Science.gov (United States)

    Temple Univ., Philadelphia, PA. Div. of Vocational Education.

    This module on the skeletal system is one of eight modules designed for individualized instruction in health occupations education programs at both the secondary and postsecondary levels. This module contains an introduction to the module topic, two objectives (e.g., list the types of joints and movements, and give examples), and two learning…

  14. Tracer verification and monitoring of containment systems (II)

    International Nuclear Information System (INIS)

    Williams, C.V.; Dunn, S.D.; Lowry, W.E.

    1997-01-01

    A tracer verification and monitoring system, SEAtrace trademark, has been designed and field tested which uses gas tracers to evaluate, verify, and monitor the integrity of subsurface barriers. This is accomplished using an automatic, rugged, autonomous monitoring system combined with an inverse optimization code. A gaseous tracer is injected inside the barrier and an array of wells outside the barrier are monitored. When the tracer gas is detected, a global optimization code is used to calculate the leak parameters, including leak size, location, and when the leak began. The multipoint monitoring system operates in real-time, can be used to measure both the tracer gas and soil vapor contaminants, and is capable of unattended operation for long periods of time (months). The global optimization code searches multi-dimensional open-quotes spaceclose quotes to find the best fit for all of the input parameters. These parameters include tracer gas concentration histories from multiple monitoring points, medium properties, barrier location, and the source concentration. SEAtrace trademark does not attempt to model all of the nuances associated with multi-phase, multi-component flow, but rather, the inverse code uses a simplistic forward model which can provide results which are reasonably accurate. The system has calculated leak locations to within 0.5 meters and leak radii to within 0.12 meters

  15. ROMANIAN BANKING SYSTEM EVOLUTION AND BASEL II REQUIREMENTS

    Directory of Open Access Journals (Sweden)

    Mihaela NICOLAU

    2006-01-01

    Full Text Available Before 1989, Romanian Banking System was structured in the specific way of a centralized economy. Restructuring of the banking system took its first step at the end of 1990 when the newly- established bank, Banca Comercială Română, took over retail operations performed previously by the NBR. Simultaneously, some privately-owned banking companies were established and foreign banks’ branches were integrated into the domestic banking activity, the number of banks almost trebling. The unfriendly economic environment, the poor quality of bank managers and shareholders and cumbersome legal procedures led to an increase in tensions, the poor quality of credit portfolio representing the major difficulty of the banking sector. In the past years, NBR tried to control more thebanking activity by implementing international settlements. More over, since Romania is one of the European Union countries, it is absolutely necessary the harmonization of entire economic and financial system to EU regulations. The paper try to present the position of Romanian banking system in the framework of all these transformations.

  16. Simulation of advanced ultrasound systems using Field II

    DEFF Research Database (Denmark)

    Jensen, Jørgen Arendt

    2004-01-01

    impulse responses is explained. A simulation example for a synthetic aperture spread spectrum flow systems is described. It is shown how the advanced coded excitation can be set up, and how the simulation can be parallelized to reduce the simulation time from 17 months to 391 hours using a 32 CPU Linux...

  17. RELIGION AND PURIFICATION OF SOUL

    Directory of Open Access Journals (Sweden)

    Azam Khodashenas Pelko

    2010-11-01

    Full Text Available The Jainism emphasizes three major teachings about the purification of the soul (jiva, Ahimsa, Aparigrapha and anekantwad. Jainism, The focus of this religion has been purification of the soul by means of right conduct, right faith and right knowledge. The ultimate goal of Hinduism is Moksha or liberation (total freedom. In Hinduism, purification of the soul is a goal that one must work to attain. The Buddhism is the science of pursuing the aim of making the human mind perfect, and of purifying the human soul. The knowledge of purifying of the soul and softening of the hearts is as essential for human. They having the correct motivations means purifying our souls from hypocrisy, caprice, and heedlessness. The primary goal of Taoism may be described as the mystical intuition of the Tao, which is the way, the undivided unity, and the ultimate Reality. According to the Christianity access to truth cannot be conceived without purity of the soul

  18. Antimicrobial Peptide Production and Purification.

    Science.gov (United States)

    Suda, Srinivas; Field, Des; Barron, Niall

    2017-01-01

    Antimicrobial peptides (AMPs) are natural defense compounds which are synthesized as ribosomal gene-encoded pre-peptides and produced by all living organisms. AMPs are small peptides, usually cationic and typically have hydrophobic residues which interact with cell membranes and have either a narrow or broad spectrum of biological activity. AMPs are isolated from the natural host or heterologously expressed in other hosts such as Escherichia coli. The proto-typical lantibiotic Nisin is a widely used AMP that is produced by the food-grade organism Lactococcus lactis. Although AMP production and purification procedures require optimization for individual AMPs, the Nisin production and purification protocol outlined in this chapter can be easily applied with minor modifications for the production and purification of other lantibiotics or AMPs. While Nisin is produced and secreted into the supernatant, steps to recover Nisin from both cell-free supernatant and cell pellet are outlined in detail.

  19. Update: Systemic Diseases and the Cardiovascular System (II). The endocrine system and the heart: a review.

    Science.gov (United States)

    Rhee, Soo S; Pearce, Elizabeth N

    2011-03-01

    Normal endocrine function is essential for cardiovascular health. Disorders of the endocrine system, consisting of hormone hyperfunction and hypofunction, have multiple effects on the cardiovascular system. In this review, we discuss the epidemiology, diagnosis, and management of disorders of the pituitary, thyroid, parathyroid, and adrenal glands, with respect to the impact of endocrine dysfunction on the cardiovascular system. We also review the cardiovascular benefits of restoring normal endocrine function. Copyright © 2010 Sociedad Española de Cardiología. Published by Elsevier Espana. All rights reserved.

  20. Operation and performance of the PEP-II prototype longitudinal damping system at ALS

    International Nuclear Information System (INIS)

    Teytelman, D.; Claus, R.; Fox, J.

    1995-05-01

    A modular programmable longitudinal feedback system has been developed as a component of the PEP-II R+D program. This system is based on a family of VME and VXI packaged signal processing functions which implement a general purpose digital feedback controller for accelerators with bunch spacings of 2 ns. A complete PEP-II prototype system has been configured and installed for use at the LBL Advanced Light Source. The system configuration used for tests at the ALS is described and results are presented showing the action of the feedback system. Open and closed loop results showing the detection and calculation of feedback signals from bunch motion are presented and the system is shown to damp coupled-bunch instabilities in the ALS. Use of the system for accelerator diagnostics is illustrated via measurement of grow-damp transients which quantify growth rates without feedback, damping rates with feedback, and identify unstable modes

  1. Chaotic time series. Part II. System Identification and Prediction

    Directory of Open Access Journals (Sweden)

    Bjørn Lillekjendlie

    1994-10-01

    Full Text Available This paper is the second in a series of two, and describes the current state of the art in modeling and prediction of chaotic time series. Sample data from deterministic non-linear systems may look stochastic when analysed with linear methods. However, the deterministic structure may be uncovered and non-linear models constructed that allow improved prediction. We give the background for such methods from a geometrical point of view, and briefly describe the following types of methods: global polynomials, local polynomials, multilayer perceptrons and semi-local methods including radial basis functions. Some illustrative examples from known chaotic systems are presented, emphasising the increase in prediction error with time. We compare some of the algorithms with respect to prediction accuracy and storage requirements, and list applications of these methods to real data from widely different areas.

  2. Continuous and distributed systems II theory and applications

    CERN Document Server

    Zgurovsky, Mikhail

    2015-01-01

    As in the previous volume on the topic, the authors close the gap between abstract mathematical approaches, such as applied methods of modern algebra and analysis, fundamental and computational mechanics, nonautonomous and stochastic dynamical systems, on the one hand, and practical applications in nonlinear mechanics, optimization, decision making theory and control theory on the other. Readers will also benefit from the presentation of modern mathematical modeling methods for the numerical solution of complicated engineering problems in biochemistry, geophysics, biology and climatology. This compilation will be of interest to mathematicians and engineers working at the interface of these fields. It presents selected works of the joint seminar series of Lomonosov Moscow State University and the Institute for Applied System Analysis at National Technical University of Ukraine “Kyiv Polytechnic Institute”. The authors come from Brazil, Germany, France, Mexico, Spain, Poland, Russia, Ukraine, and the USA. ...

  3. Partial Purification and Characterization of Extracellular Protease ...

    African Journals Online (AJOL)

    Nigerian Journal of Basic and Applied Sciences ... Purification of the enzyme by gel filtration chromatography on Sephadex G75 following ammonium sulphate precipitation gave 2.26 fold increase in purification with specific activity of 46.13 units/mg protein while purification on Sephadex CM50 resulted in reduced ...

  4. T-6A Texan II Systems Engineering Case Study

    Science.gov (United States)

    2010-01-01

    techniques. DAPPs could implement the provisions of FASA before the regulations were published and could use commercial item exemptions for non... IFR Certified Instrumentation IFR Certified (Selectable EADI/EHSI) All digital except backups Visual System for IFT/OFT Provide a visual field...accomplished via AF Form 1067) is required for any new projects/issues introduced at the User’s JPL Meeting. This User meeting will consolidate /prioritize all

  5. BOA II: pipe-asbestos insulation removal system

    International Nuclear Information System (INIS)

    Schempf, H.; Mutschler; Boehmke, S.; Chemel, B.; Piepgras, C.

    1996-01-01

    BOA system is a mobile pipe-external robotic crawler used to remotely strip and bag asbestos-containing lagging and insulation materials from various diameter pipes in (primarily) industrial installations. Steam and process lines within the DOE weapons complex warrant the use of a remote device due to high labor costs and high level of radioactive contamination, making manual removal costly and inefficient. Currently targeted facilities for demonstration and remediation are Fernald in Ohio and Oak Ridge in Tennessee

  6. Power train and emission control: allocation procedure by OBD-II system for automotive technology

    Science.gov (United States)

    Kalita, Porag

    2017-06-01

    OBD-II, systems were designed to maintain low emissions of in use vehicles, including light and medium duty vehicles. In 1989, the California code of Regulations (CCR) known as OBD - II was adopted by the California Air Resource Board (CARB) and the objective to reduce hydrocarbon (HC) emission caused by malfunction of the vehicles emission control systems. OBD-II provides additional information to engineer for diagnosis and repair of emissions related problems. OBD-II, standardizes on the amount of memory (Freeze Frame) it uses to store the readings of the vehicle sensor when it logs on emission related Intermittent Trouble code (IT). The intent of OBD-II, systems is to detect most vehicle malfunctions when performance of a power train component or system deteriorates to the point that the vehicle’s HC emission exceed standard. The vehicle operator is notified at the time when the vehicle begins to marginally exceed emission standards, by illuminating the Malfunctions Indicator Light (MIL).

  7. High speed phototimer and biplane II blanking method in 105 mm RSC photographing of circulatory system

    International Nuclear Information System (INIS)

    Kaga, Yuji; Kiuchi, Shigeo; Sato, Masami; Komatsuda, Yasushi; Nishio, Kosaku.

    1979-01-01

    A high speed phototimer as an autoexposure mechanism was developed for 105 mm II indirect continuous photographing of circulatory system. The phototimer can give repeated response of 12 times/sec and the shortest X-ray shut out of 1 m sec. The proper lighting field for the phototimer is 7 mm diameter (12%) of the II input area, equivalent to 80 mm in diameter), and the tube voltage, object property and the focus-II distance characteristics are all well corrected to give good photographs of optimal density. The mutual fog in biplane photographing can be removed by adopting II blanking method. As blanking can respond quite rapidly, photographing time plus 2 m sec is enough for blanking time. That means the positive phase can be brought close to 3 m sec. This mechanism can be applied for biplane cine-photographing. (Kobatake, H.)

  8. The Chimera II Real-Time Operating System for advanced sensor-based control applications

    Science.gov (United States)

    Stewart, David B.; Schmitz, Donald E.; Khosla, Pradeep K.

    1992-01-01

    Attention is given to the Chimera II Real-Time Operating System, which has been developed for advanced sensor-based control applications. The Chimera II provides a high-performance real-time kernel and a variety of IPC features. The hardware platform required to run Chimera II consists of commercially available hardware, and allows custom hardware to be easily integrated. The design allows it to be used with almost any type of VMEbus-based processors and devices. It allows radially differing hardware to be programmed using a common system, thus providing a first and necessary step towards the standardization of reconfigurable systems that results in a reduction of development time and cost.

  9. Anthology of the renin-angiotensin system: a one hundred reference approach to angiotensin II antagonists.

    Science.gov (United States)

    Ménard, J

    1993-04-01

    To provide a historical overview of the renin-angiotensin system as a guide to the introduction of a new therapeutic pathway, non-peptide inhibition of a angiotensin II. One hundred references were selected as a personal preference, for their originality or for their potential impact on medicine. This review raises the following questions for future research. (1) Will the long-term cardiovascular effects of angiotensin converting enzyme (ACE) inhibition, angiotensin II antagonism and renin inhibition be similar or not, and dependent or independent of blood pressure levels? (2) What are the local-regional interactions between vasoconstrictor and vasodilator systems, and does the renin-angiotensin system synchronize these regional hemodynamic regulatory mechanisms? (3) If hypertension is the result of an interaction between genetic and environmental factors, do proteins secreted through constitutive pathways contribute to the genetic abnormality (prorenin, angiotensinogen, ACE) while regulated secretion (renin) and other regulatory mechanisms (angiotensin II receptors) provide biological support for the environmental effects?

  10. Materials for Molybdenum 99 purification

    International Nuclear Information System (INIS)

    Wilkinson, M. Victoria; Mondino, Angel V.; Manzini, Alberto C.

    2003-01-01

    The National Atomic Energy Commission (CNEA) produces fission Mo 99, an isotope of wide use in nuclear medicine. In order to simplify the current Mo 99 production process, to shorten its duration and reduce impurities in the final product, alternative methods for purification steps were looked for. In this work a variety of new materials for the purification columns were designed, all of them with carbon. These materials were studied and a material which contribute with the best results for molybdenum retention, was selected. The preparation procedure and the working conditions were determined. (author)

  11. Hydrogen purification by periodic adsorption

    Energy Technology Data Exchange (ETDEWEB)

    Barg, Christian; Secchi, Argimiro R.; Trierweiler, Jorge O. [Rio Grande do Sul Univ., Porto Alegre, RS (Brazil). Dept. de Engenharia Quimica]. E-mail: cbarg@enq.ufrgs.br; arge@enq.ufrgs.br; jorge@enq.ufrgs.br

    2000-07-01

    The periodic adsorption processes have been widely used for industrial applications, mainly because it spends less energy than the usual gas separation processes, like the cryogenic distillation. The largest commercial application of periodic adsorption processes is the pressure swing adsorption (PSA) applied to hydrogen purification. Although its wide use in the chemical and petrochemical industry, there are no reports in the open literature about complete modeling studies of a complex commercial unit, with multiple adsorbents and multiple beds and several feed components. This study has as objective the modeling, optimization and dynamical analysis of an industrial PSA unit for hydrogen purification. (author)

  12. Fail-safety of the EBR-II steam generator system

    International Nuclear Information System (INIS)

    Chopra, P.S.; Stone, C.C.; Hutter, E.; Barney, W.K.; Staker, R.G.

    1976-01-01

    Fail-safe analyses of the EBR-II steam-generator system show that a postulated non-instantaneous leak of water or steam into sodium, through a duplex tube or a tubesheet, at credible leak rates will not structurally damage the evaporators and superheaters. However, contamination of the system and possible shell wastage by sodium-water reaction products may render the system inoperable for a period exceeding six months. This period would be shortened to three months if the system were modified by adding a remotely operated water dump system, a steam vent system, a secondary sodium superheater relief line, and a tubesheet leak-detection system

  13. Effect of water purification process in radioactive content: analysis on small scale purification plants

    International Nuclear Information System (INIS)

    Lopez del Rio, H.; Quiroga S, J. C.; Davila R, J. I.; Mireles G, F.

    2009-10-01

    Water from small scale purification plants is a low cost alternative for consumers in comparison to the bottled commercial presentations. Because of its low cost per liter, the consumption of this product has increased in recent years, stimulating in turn the installation of purification systems for these small businesses. The purpose of this study was to estimate the efficiency of small scale purification systems located in the cities of Zacatecas and Guadalupe, Zacatecas, to reduce the radioactive content of water. It was measured the total alpha and beta activity in water samples of entry and exit to process, through the liquid scintillation technique. In general it was observed that the process is more efficient in removing alpha that beta activity. The fraction of total alpha activity removed varied between 27 and 100%, while between 0 and 77% of the total beta activity was removed by the analyzed plants. In all cases, the total radioactivity level was lower than the maximum permissible value settled by the official mexican standard for drinking water. (Author)

  14. Analyse in the patients with refractory heart purification treated by continuous blood purification

    International Nuclear Information System (INIS)

    Cai Xun; Huang Chengwen; Huang Zhongliang

    2006-01-01

    Objective: To investigate the application of continuous blood purification (CBP) and the effect of the angiotensin II (Ang II), aldosterone (ALD), atrial natriuretic peptide (ANP) in plasma with refractory heart failure (RHF) treated by CBP. Methods: 35 cases with RHF were treated by CBP. Blood pressure, heart rate, respiration, blood oxygen saturation, blood markers of biochemistry, blood gas analysis, left ventricle ejection fraction (LVEF), cardiac function and Ang II, ALD, ANF in plasma were checked before and after treatment. Results: the systolic pressure, diastolic pressure, heart rate, respiration, serum creatinine, blood urea nitroge, Ang II, ALD, ANF in plasma after treatment were significantly lower than those before treatment of CBP (P 3 - , PaO 2 , LVEF after treatment were significantly higher than those before treatment of CBP (P + , Na + , Cl - of blood serum were recovered normal after treatment, and cardiac function was recovered (P<0.05, P<0.01). Conclusion: In the patients with RHF, CBP can efficiently treat the water-electrolyte disturbance, keep the acid-base balance, decrease the levels of AngII, ALD, ANF in plasma, clear the inflammatory mediator and improve cardiac function. (authors)

  15. Planetary Data Systems (PDS) Imaging Node Atlas II

    Science.gov (United States)

    Stanboli, Alice; McAuley, James M.

    2013-01-01

    The Planetary Image Atlas (PIA) is a Rich Internet Application (RIA) that serves planetary imaging data to the science community and the general public. PIA also utilizes the USGS Unified Planetary Coordinate system (UPC) and the on-Mars map server. The Atlas was designed to provide the ability to search and filter through greater than 8 million planetary image files. This software is a three-tier Web application that contains a search engine backend (MySQL, JAVA), Web service interface (SOAP) between server and client, and a GWT Google Maps API client front end. This application allows for the search, retrieval, and download of planetary images and associated meta-data from the following missions: 2001 Mars Odyssey, Cassini, Galileo, LCROSS, Lunar Reconnaissance Orbiter, Mars Exploration Rover, Mars Express, Magellan, Mars Global Surveyor, Mars Pathfinder, Mars Reconnaissance Orbiter, MESSENGER, Phoe nix, Viking Lander, Viking Orbiter, and Voyager. The Atlas utilizes the UPC to translate mission-specific coordinate systems into a unified coordinate system, allowing the end user to query across missions of similar targets. If desired, the end user can also use a mission-specific view of the Atlas. The mission-specific views rely on the same code base. This application is a major improvement over the initial version of the Planetary Image Atlas. It is a multi-mission search engine. This tool includes both basic and advanced search capabilities, providing a product search tool to interrogate the collection of planetary images. This tool lets the end user query information about each image, and ignores the data that the user has no interest in. Users can reduce the number of images to look at by defining an area of interest with latitude and longitude ranges.

  16. ELECTRONIC SYSTEM FOR EXPERIMENTATION IN AC ELECTROGRAVIMETRY II: IMPLEMENTED DESIGN

    Directory of Open Access Journals (Sweden)

    Robinson Torres

    2007-06-01

    Full Text Available A detailed description of the electronic system designed to improve the measurements in an experimental AC electrogravimetry setup is presented. This system is committed to acquire appropriated data for determining the Electrogravimetric Transfer Function (EGTF and provide information regarding the mass transfer in an electrochemical cell in the AC Electrogravimetry Technique, but maintaining a good trade-off between the locking frequency bandwidth and the resolution in the frequency tracking, that is, enlarging the bandwidth of the system to follow signals with frequency as higher as 1 kHz, but maintaining an accurate and continuous tracking of this signal. The enlarged bandwidth allows the study of fast kinetic process in electrochemical applications and the continuous tracking let to achieve a precise measurement with good resolution rather than average frequency records obtained by conventional frequency meters. The system is based on an Analogue-Digital Phase Locked Loop (A-D PLL.En este artículo se presenta una descripción detallada del sistema electrónico diseñado para mejorar las medidas en un sistema experimental de electrogravimetría AC. El sistema diseñado se encarga de adquirir los datos adecuados para determinar la función de transferencia electrogravimétrica (EGTF y proveer información relacionada con la transferencia de masa en una celda electroquímica en la técnica de electrogravimetría AC, pero manteniendo un buen compromiso entre el ancho de banda de enganche y la resolución en el seguimiento de la frecuencia, es decir, el sistema incrementa el ancho de banda para permitir el seguimiento de señales con frecuencias hasta de 1 kHz, pero conservando un exacto y continuo seguimiento de esta señal. El aumento del ancho de banda permite el estudio de procesos con una cinética rápida en aplicaciones electroquímicas y el seguimiento continuo de la señal permite la obtención de medidas precisas con buena resoluci

  17. Microemulsion systems studied by positron annihilation techniques. II

    International Nuclear Information System (INIS)

    Lopez, R.; Olea, O.; Fucugauchi, L.A.

    1982-01-01

    The positron annihilation technique was applied to assess the effect of the double bond of the surfactant molecule on the microemulsion formation mechanism by replacing sodium stearate by sodium oleate in surfactant-cosurfactant-solvent-water systems. Diameters of microemulsions were measured by dynamic laser light scattering, and the obtained values concur with the present positron annihilation data. Structures of these microemulsions have been assessed by considering the available conformation and shapes of the surfactant-cosurfactant-solvent-assemblies. Volumes of the same aggregates have been calculated by taking into consideration the packing constraint imposed upon kink formation. (Auth.)

  18. Physical habitat simulation system reference manual: version II

    Science.gov (United States)

    Milhous, Robert T.; Updike, Marlys A.; Schneider, Diane M.

    1989-01-01

    There are four major components of a stream system that determine the productivity of the fishery (Karr and Dudley 1978). These are: (1) flow regime, (2) physical habitat structure (channel form, substrate distribution, and riparian vegetation), (3) water quality (including temperature), and (4) energy inputs from the watershed (sediments, nutrients, and organic matter). The complex interaction of these components determines the primary production, secondary production, and fish population of the stream reach. The basic components and interactions needed to simulate fish populations as a function of management alternatives are illustrated in Figure I.1. The assessment process utilizes a hierarchical and modular approach combined with computer simulation techniques. The modular components represent the "building blocks" for the simulation. The quality of the physical habitat is a function of flow and, therefore, varies in quality and quantity over the range of the flow regime. The conceptual framework of the Incremental Methodology and guidelines for its application are described in "A Guide to Stream Habitat Analysis Using the Instream Flow Incremental Methodology" (Bovee 1982). Simulation of physical habitat is accomplished using the physical structure of the stream and streamflow. The modification of physical habitat by temperature and water quality is analyzed separately from physical habitat simulation. Temperature in a stream varies with the seasons, local meteorological conditions, stream network configuration, and the flow regime; thus, the temperature influences on habitat must be analysed on a stream system basis. Water quality under natural conditions is strongly influenced by climate and the geological materials, with the result that there is considerable natural variation in water quality. When we add the activities of man, the possible range of water quality possibilities becomes rather large. Consequently, water quality must also be analysed on a

  19. Investigation of the Surface Properties of an Oxide of Interest in the Field of a Conductive Oxide System: Influence of Precursor and Purification

    Directory of Open Access Journals (Sweden)

    Gomes Gilmar de Almeida

    1999-01-01

    Full Text Available The surface properties of commercial TiO2 and samples synthesized by the thermal decomposition procedure using several precursor salts were investigated by a microelectrophoresis technique. The iep was determined as a function of the ionic strength which was controlled with KNO3 or KCl. The experimental results showed: 1 The commercial sample and the ones synthesized from the chloride salt dissolved in HCl 1:1 (v/v show low iep?s due to chloride inclusion; 2 The purification methods explored (oxide suspension at pH~12 and dialysis are inefficient; 3 TiO2 samples synthesized from an organic precursor salt under totally chloride-free conditions furnished an iep of 5.9 in excellent agreement with literature data; 4 Chloride specific adsorption on TiO2 is weak.

  20. An embedded EEG analyzing system based on muC/os-II.

    Science.gov (United States)

    Liu, Boqiang; Zhang, Yanyan; Liu, Zhongguo; Yin, Cong

    2007-01-01

    An EEG analyzing system based on Advanced RISC Machines (ARM) and muC/os-II real time operating system is discussed in this paper. The detailed system design including the producing of event signals and the synchronization between event signals and EEG signals is described. The details of data acquisition, data preprocessing, data transmitting through USB and system configurations are also contained in the system design. In this paper the design of high capability amplifier and the software of embedded subsystem are discussed. Also the design of realizing multi-task system in muC/os-II, the definition of communicating protocols between PC and the equipment and the detail configurations of USB are given out. The final test shows that the filter behaviors of this equipment are feasible.

  1. EBR-II secondary sodium loop Plugging Temperature Indicator control system upgrade

    International Nuclear Information System (INIS)

    Carlson, R.B.; Gehrman, R.L.

    1995-01-01

    The Experimental Breeder Reactor II (EBR-II) secondary sodium coolant loop Plugging Temperature Indicator (PTI) control system was upgraded in 1993 to a real-time computer based system. This was done to improve control, to remove obsolete and high maintenance equipment, and to provide a graphical CRT based operator interface. A goal was to accomplish this inexpensively using small, reliable computer and display hardware with a minimum of purchased software. This paper describes the PTI system, the upgraded control system and its operator interface, and development methods and tools. The paper then assesses how well the system met its goals, discusses lessons learned and operational improvements noted, and provides some recommendations and suggestions on applying small real-time control systems of this type

  2. Diagnosing and curing system freeze-ups: Part II

    Energy Technology Data Exchange (ETDEWEB)

    Frey, R. [Heating and Plumbing Service, Sparta, NJ (United States)

    1996-02-01

    In our last article, we discussed determination and possible causes leading to frozen pipes in the heating system. We now turn to the matter of dealing with such freeze-ups. There are two major categories of frozen heating systems. The first and worst we can label the {open_quotes}Floridian Flew{close_quotes} type, when the usual occupants have taken off for warmer climates. The second is the {open_quotes}Howcudit {open_quote}B{close_quote} virus,{close_quotes} which occurs to normal people under an unusual Arctic Attack right while they are living there. Regarding the first, I never cease to be amazed how folks can just up and leave their house for three days to three months without making proper arrangements for monitoring the temperature of their home during their absence. There are a frightening number of humanoids out there, who either lack a minimal presence of common sense, or who are terrible gamblers. The amazing thing is that some of these folks wouldn`t even gamble a postage stamp on a magazine sweepstakes, but still will gamble the contents of their home by driving off for two weeks at Disneyland in the middle of January without ever making any provision for assuring that their house doesn`t freeze up while they are away. The {open_quotes}Howcudit {open_quote}B{close_quote}{close_quotes} type is not nearly as devasting as the {open_quotes}Floridian Flew.{close_quotes} Imagine coming home to an icy cold house after flying in from the sunny beaches of some tropical paradise. The oilburner switch is on. The thermostate is set at 60 where we left it, but the needle is buried somewhere down behind the cover, like it is cowering from guilt. {open_quotes}Oh Man! I just checked the bathroom; there is an icicle hanging from the vanity faucet and the toilet bowl is a block of ice.{close_quotes}

  3. Unmanned Aircraft Systems (UAS) Traffic Management (UTM) National Campaign II

    Science.gov (United States)

    Aweiss, Arwa S.; Owens, Brandon D.; Rios, Joseph L.; Homola, Jeffrey R.; Mohlenbrink, Christoph P.

    2018-01-01

    The Unmanned Aircraft System (UAS) Traffic Management (UTM) effort at NASA aims to enable access to low-altitude airspace for small UAS. This goal is being pursued partly through partnerships that NASA has developed with the UAS stakeholder community, the FAA, other government agencies, and the designated FAA UAS Test Sites. By partnering with the FAA UAS Test Sites, NASA's UTM project has performed a geographically diverse, simultaneous set of UAS operations at locations in six states. The demonstrations used an architecture that was developed by NASA in partnership with the FAA to safely coordinate such operations. These demonstrations-the second or 'Technical Capability Level (TCL 2)' National Campaign of UTM testing-was performed from May 15 through June 9, 2017. Multiple UAS operations occurred during the testing at sites located in Alaska, Nevada, Texas, North Dakota, Virginia, and New York with multiple organizations serving as UAS Service Suppliers and/or UAS Operators per the specifications provided by NASA. By engaging various members of the UAS community in development and operational roles, this campaign provided initial validation of different aspects of the UTM concept including: UAS Service Supplier technologies and procedures; geofencing technologies/conformance monitoring; ground-based surveillance/sense and avoid; airborne sense and avoid; communication, navigation, surveillance; and human factors related to UTM data creation and display. Additionally, measures of performance were defined and calculated from the flight data to establish quantitative bases for comparing flight test activities and to provide potential metrics that might be routinely monitored in future operational UTM systems.

  4. Angiotensin II dependent cardiac remodeling in the eel Anguilla anguilla involves the NOS/NO system

    DEFF Research Database (Denmark)

    Filice, Mariacristina; Amelio, Daniela; Garofalo, Filippo

    2017-01-01

    Angiotensin II (AngII), the principal effector of the Renin-Angiotensin System (RAS), plays an important role in controlling mammalian cardiac morpho-functional remodelling. In the eel Anguilla anguilla, one month administration of AngII improves cardiac performance and influences the expression ...

  5. Purification of a synthetic pterocarpanquinone by countercurrent chromatography

    International Nuclear Information System (INIS)

    Costa, Fernanda das Neves; Silva, Alcides Jose M. da; Domingos, Jorge L. de Oliveira; Costa, Paulo Roberto R.; Leitao, Gilda G.; Daher Netto, Chaquip

    2012-01-01

    Countercurrent chromatography (CCC) was employed as a useful, fast and economic alternative to conventional chromatography techniques for the purification of a synthetic pterocarpanquinone, LQB-118. The separation was performed in a two-step CCC with the solvent system hexanechloroform- methanol-water 2:1.5:5:2 in both steps. Traditional purification of these reaction products by silica gel column chromatography demanded a large amount of solvent and time, besides allowing the irreversible adsorption of the compound in the column. The use of 1 H NMR for the calculation of KD of target compound is proposed as an alternative for HPLC measurements. (author)

  6. Expression and purification of recombinant nattokinase in Spodoptera frugiperda cells.

    Science.gov (United States)

    Li, Xiaoxiang; Wang, Xiaoli; Xiong, Shaoling; Zhang, Jing; Cai, Litao; Yang, Yanyan

    2007-10-01

    A recombinant baculovirus, rv-egfp-NK, containing a reporter gene encoding the enhanced green fluorescent protein (EGFP), was used to express nattokinase (NK), a fibrinolytic enzyme, in Spodoptera frugiperda (SF-9) cells. The recombinant protein also included a histidine tag for purification using Ni(2+) resins. The recombinant NK, approximately 30 kDa, retained fibrinolytic activity (60 U/ml). The integration of the EGFP expression cassette in the Bac-to-Bac system is thus an effective method for the expression and purification of recombinant NK protein in Spodoptera frugiperda insect cells.

  7. A Photo Storm Report Mobile Application, Processing/Distribution System, and AWIPS-II Display Concept

    Science.gov (United States)

    Longmore, S. P.; Bikos, D.; Szoke, E.; Miller, S. D.; Brummer, R.; Lindsey, D. T.; Hillger, D.

    2014-12-01

    The increasing use of mobile phones equipped with digital cameras and the ability to post images and information to the Internet in real-time has significantly improved the ability to report events almost instantaneously. In the context of severe weather reports, a representative digital image conveys significantly more information than a simple text or phone relayed report to a weather forecaster issuing severe weather warnings. It also allows the forecaster to reasonably discern the validity and quality of a storm report. Posting geo-located, time stamped storm report photographs utilizing a mobile phone application to NWS social media weather forecast office pages has generated recent positive feedback from forecasters. Building upon this feedback, this discussion advances the concept, development, and implementation of a formalized Photo Storm Report (PSR) mobile application, processing and distribution system and Advanced Weather Interactive Processing System II (AWIPS-II) plug-in display software.The PSR system would be composed of three core components: i) a mobile phone application, ii) a processing and distribution software and hardware system, and iii) AWIPS-II data, exchange and visualization plug-in software. i) The mobile phone application would allow web-registered users to send geo-location, view direction, and time stamped PSRs along with severe weather type and comments to the processing and distribution servers. ii) The servers would receive PSRs, convert images and information to NWS network bandwidth manageable sizes in an AWIPS-II data format, distribute them on the NWS data communications network, and archive the original PSRs for possible future research datasets. iii) The AWIPS-II data and exchange plug-ins would archive PSRs, and the visualization plug-in would display PSR locations, times and directions by hour, similar to surface observations. Hovering on individual PSRs would reveal photo thumbnails and clicking on them would display the

  8. Macaque accessory optic system: II. Connections with the pretectum

    International Nuclear Information System (INIS)

    Baleydier, C.; Magnin, M.; Cooper, H.M.

    1990-01-01

    Connections of the accessory optic system (AOS) with the pretectum are described in the macaque monkey. Injections of tritiated amino acids in the pretectum demonstrate a major contralateral projection to the dorsal (DTN), lateral (LTN), and medial (MTN) terminal nuclei of the AOS and a sparser projection to the ipsilateral LTN. Injections of retrograde tracers, Fast Blue (FB), or wheat germ agglutinin horseradish peroxidase (WGA-HRP) plus nonconjugated horseradish peroxidase (HRP) in the LTN show that the pretectal-LTN projection originates from two nuclei. The main source of pretectal efferents to the LTN is from the pretectal olivary nucleus (OPN) and is entirely contralateral. This projection, which appears unique to primates, originates from the large multipolar cells of the OPN. In addition to this projection, the nucleus of the optic tract (NOT) projects to the ipsilateral LTN, as in nonprimates. Injection of WGA-HRP in the pretectum shows a reciprocal predominantely ipsilateral projection from the LTN to the pretectum. Retinas were observed after injection of FB in the LTN. The retinal ganglion cells projecting to the AOS are mainly distributed near the fovea and in the nasal region of the contralateral eye, suggesting a nasotemporal pattern of decussation. The demonstration of a direct connection between LTN and OPN forces to a reconsideration of the functional role of the AOS. Previous descriptions of luminance responsive cells in the LTN support a possible participation of this nucleus in the control of the pupillary light reflex

  9. Mars Hybrid Propulsion System Trajectory Analysis. Part II; Cargo Missions

    Science.gov (United States)

    Chai, Patrick R.; Merrill, Raymond G.; Qu, Min

    2015-01-01

    NASA's Human Spaceflight Architecture Team is developing a reusable hybrid transportation architecture in which both chemical and electric propulsion systems are used to send crew and cargo to Mars destinations such as Phobos, Deimos, the surface of Mars, and other orbits around Mars. By combining chemical and electrical propulsion into a single spaceship and applying each where it is more effective, the hybrid architecture enables a series of Mars trajectories that are more fuel-efficient than an all chemical architecture without significant increases in flight times. This paper shows the feasibility of the hybrid transportation architecture to pre-deploy cargo to Mars and Phobos in support of the Evolvable Mars Campaign crew missions. The analysis shows that the hybrid propulsion stage is able to deliver all of the current manifested payload to Phobos and Mars through the first three crew missions. The conjunction class trajectory also allows the hybrid propulsion stage to return to Earth in a timely fashion so it can be reused for additional cargo deployment. The 1,100 days total trip time allows the hybrid propulsion stage to deliver cargo to Mars every other Earth-Mars transit opportunity. For the first two Mars surface mission in the Evolvable Mars Campaign, the short trip time allows the hybrid propulsion stage to be reused for three round-trip journeys to Mars, which matches the hybrid propulsion stage's designed lifetime for three round-trip crew missions to the Martian sphere of influence.

  10. HYLIFE-II power conversion system design and cost study

    International Nuclear Information System (INIS)

    Hoffman, M.A.

    1990-09-01

    The power conversion system for the HYLIFE-2 fusion power plant has been defined to include the IHX's (intermediate heat exchangers) and everything that support the exchange of energy from the reactor. It is referred to simply as the BOP (balance of plant) in the rest of this report. The above is a convenient division between the reactor equipment and the rest of the fusion power plant since the BOP design and cost then depend only on the specification of the thermal power to the IHX's and the temperature of the primary Flibe coolant into and out of the IHX's, and is almost independent of the details of the reactor design. The main efforts during the first year have been on the definition and thermal-hydraulics of the IHX's, the steam generators and the steam power plant, leading to the definition of a reference BOP with the molten salt, Flibe, as the primary coolant. A summary of the key results in each of these areas is given in this report

  11. Introduction to integrable many-body systems II

    International Nuclear Information System (INIS)

    Samaj, L.

    2010-01-01

    This is the second part of a three-volume introductory course about integrable systems of interacting bodies. The models of interest are quantum spin chains with nearest-neighbor interactions between spin operators, in particular Heisenberg spin- 2 models. The Ising model in a transverse field, expressible as a quadratic fermion form by using the Jordan-Wigner transformation, is the subject of Sect. 12. The derivation of the coordinate Bethe ansatz for the XXZ Heisenberg chain and the determination of its absolute ground state in various regions of the anisotropy parameter are presented in Sect. 13. The magnetic properties of the ground state are explained in Sect. 14. Sect. 15 concerns excited states and the zero-temperature thermodynamics of the XXZ model. The thermodynamics of the XXZ Heisenberg chain is derived on the basis of the string hypothesis in Sect. 16; the thermodynamic Bethe ansatz equations are analyzed in high-temperature and low-temperature limits. An alternative derivation of the thermodynamics without using strings, leading to a non-linear integral equation determining the free energy, is the subject of Sect. 17. A nontrivial application of the Quantum Inverse Scattering method to the fully anisotropic XYZ Heisenberg chain is described in Section 18. Section 19 deals with integrable cases of isotropic spin chains with an arbitrary spin. (Author)

  12. Regulation of aortic extracellular matrix synthesis via noradrenergic system and angiotensin II in juvenile rats.

    Science.gov (United States)

    Dab, Houcine; Hachani, Rafik; Dhaouadi, Nedra; Sakly, Mohsen; Hodroj, Wassim; Randon, Jacques; Bricca, Giampiero; Kacem, Kamel

    2012-10-01

    Extracellular matrix (ECM) synthesis regulation by sympathetic nervous system (SNS) or angiotensin II (ANG II) was widely reported, but interaction between the two systems on ECM synthesis needs further investigation. We tested implication of SNS and ANG II on ECM synthesis in juvenile rat aorta. Sympathectomy with guanethidine (50 mg/kg, subcutaneous) and blockade of the ANG II AT1 receptors (AT1R) blocker with losartan (20 mg/kg/day in drinking water) were performed alone or in combination in rats. mRNA and protein synthesis of collagen and elastin were examined by Q-RT-PCR and immunoblotting. Collagen type I and III mRNA were increased respectively by 62 and 43% after sympathectomy and decreased respectively by 31 and 60% after AT1R blockade. Combined treatment increased collagen type III by 36% but not collagen type I. The same tendency of collagen expression was observed at mRNA and protein levels after the three treatments. mRNA and protein level of elastin was decreased respectively by 63 and 39% and increased by 158 and 15% after losartan treatment. Combined treatment abrogates changes induced by single treatments. The two systems act as antagonists on ECM expression in the aorta and combined inhibition of the two systems prevents imbalance of mRNA and protein level of collagen I and elastin induced by single treatment. Combined inhibition of the two systems prevents deposit or excessive reduction of ECM and can more prevent cardiovascular disorders.

  13. Evaluating the response of complex systems to environmental threats: the Σ II method

    International Nuclear Information System (INIS)

    Corynen, G.C.

    1983-05-01

    The Σ II method was developed to model and compute the probabilistic performance of systems that operate in a threatening environment. Although we emphasize the vulnerability of complex systems to earthquakes and to electromagnetic threats such as EMP (electromagnetic pulse), the method applies in general to most large-scale systems or networks that are embedded in a potentially harmful environment. Other methods exist for obtaining system vulnerability, but their complexity increases exponentially as the size of systems is increased. The complexity of the Σ II method is polynomial, and accurate solutions are now possible for problems for which current methods require the use of rough statistical bounds, confidence statements, and other approximations. For super-large problems, where the costs of precise answers may be prohibitive, a desired accuracy can be specified, and the Σ II algorithms will halt when that accuracy has been reached. We summarize the results of a theoretical complexity analysis - which is reported elsewhere - and validate the theory with computer experiments conducted both on worst-case academic problems and on more reasonable problems occurring in practice. Finally, we compare our method with the exact methods of Abraham and Nakazawa, and with current bounding methods, and we demonstrate the computational efficiency and accuracy of Σ II

  14. Implementation of MOAS II diagnosis system at the OECD Halden Reactor project

    International Nuclear Information System (INIS)

    Kim, I.S.; Grini, R.E.; Nilsen, S.

    1995-01-01

    MOAS II is a surveillance and diagnosis system that uses several techniques for knowledge acquisition and diagnostic reasoning, e.g., goal tree-success tree, simplified directed graphs, diagnosis trees, and detailed knowledge of the process, such as mass or energy balance. This new approach was used at the Halden Man-Machine Laboratory of the OECD Halden Reactor Project. The performance of MOAS II, developed in G2 real-time expert system shell for the high-pressure preheaters of the NORS process, was tested against a variety of transient scenarios, including failures of control valves and sensors, and leakage of tubes of the preheaters. These tests showed that MOAS II successfully carried out its intended functions, i.e., quickly recognizing an occurring disturbance, correctly diagnosing its cause, and presenting advice on its control to the operator. The insights gained during the implementation are discussed

  15. [Medical hemostasis. II. Systemic hemostatics, a critical evaluation].

    Science.gov (United States)

    van den Bogaard, A E

    1989-02-01

    The control of spontaneous and traumatic haemorrhage is a matter of constant concern to veterinary practitioners. In some instances, the control of bleeding may be relatively simple using some topical therapeutic procedure, but when haemostatic defects are present, treatment with topical agents alone might not be sufficient, and more radical and life-saving procedures are indicated: replacement therapy with blood or blood products. As this is not easy to perform in most veterinary clinical situations, any therapeutic agent, which facilitates control of haemorrhage is a welcome addition to the therapeutic armament. Besides vitamin K, blood products and agents for topical use, there are only a few agents having a well-defined action on the mechanism of haemostasis. On the other hand several drugs are propagated in the Netherlands as systemic haemostatics in the treatment of bleeding disorders and prevention of haemorrhage during operation. None of these is of proven clinical value in veterinary practice. The only agent, which possibly showed some clinical effect: ethamsylate, has recently been withdrawn from the Dutch market by the manufacturers. Double-blind prospective trials with quantitation of loss of blood showed that antifibrinolytic agents such as tranexamic acid are clinically effective in certain conditions in humans. Because of their mode of action, it might reasonably be expected that these drugs might have positive results in veterinary medicine. Therefore, clinical trials with antifibrinolytic agents are urgently indicated to evaluate the effectiveness and side-effects of these drugs in animals showing severe bleeding or in the prevention of peroperative haemorrhage.

  16. Perovskite phases in the systems Asup(II)O-Usup(VI)O/sub 3/. 2. On the system A/sub 2/sup(II)Bsup(II)Usup(VI)O/sub 6/, with Asup(II), Bsup(II) = Ba, Sr, Ca

    Energy Technology Data Exchange (ETDEWEB)

    Griffiths, A J; Kemmler-Sack, S [Tuebingen Univ. (Germany, F.R.). Lehrstuhl fuer Anorganische Chemie 2

    1980-07-01

    Studies on the system A/sub 2/sup(II)Bsup(II)Usup(VI)O/sub 6/ with Asup(II), Bsup(II) = Ba, Sr, Ca or combinations of these have shown that the alkaline earth ions cannot substitute each other in all proportions. The perovskites were studied by X-ray diffraction and vibrational spectroscopic methods. The lattice vibration region of the far infrared spectra proved to be of particular value in providing information on the occupancy of the A and B sites. Analysis of the spectra shows that, in the majority of cases, contary to the geometrical predictions some of the larger alkaline earth ions occupy the six-coordinated B sites and some smaller ions the A positions. The number of ions that take in this A reversible B site-exchange can amount to 20%, but is normally smaller.

  17. Copper(II) ions interactions in the systems with triamines and ATP. Potentiometric and spectroscopic studies.

    Science.gov (United States)

    Hoffmann, S K; Goslar, J; Bregier-Jarzebowska, R; Gasowska, A; Zalewska, A; Lomozik, L

    2017-12-01

    The mode of interaction and thermodynamic stability of complexes formed in binary and ternary Cu(II)/ATP/triamines systems were studied using potentiometric and spectroscopic (NMR, EPR, UV-Vis) methods. It was found that in binary metal-free systems ATP/H x PA species are formed (PA: Spd=spermidine or 3,3-tri=1,7-diamino-4-azaheptane) where the phosphate groups from nucleotides are preferred negative centers and protonated amine groups of amines are positive centers of reaction. In the ternary systems Cu/ATP/H x (PA) as well as Cu/(ATP)(PA) species are formed. The type of the formed Cu(II) complexes depends on pH of the solution. For a low pH value the complexation appears between Cu(II) and ATP molecules via oxygen atoms of phosphate groups. For a very high pH value, where ATP is hydrolyzed, the Cu(II) ions are bound to the nitrogen atoms of polyamine molecules. We did not detect any direct coordination of the N7 nitrogen atom of adenosine to Cu(II) ions. It means that the CuN7 interaction is an indirect type and can be due to noncovalent interplay including water molecule. EPR studies were performed at glassy state (77K) after a fast freezing both for binary and ternary systems. The glassy state EPR spectra do not reflect species identified in titration studies indicating significant effect of rapid temperature decrease on equilibrium of Cu(II) complexes. We propose the molecular structure of all the studied complexes at the glassy state deduced from EPR and optical spectroscopy results. Copyright © 2017 Elsevier Inc. All rights reserved.

  18. 25 CFR 547.12 - What are the minimum technical standards for downloading on a Class II gaming system?

    Science.gov (United States)

    2010-04-01

    ... on a Class II gaming system? 547.12 Section 547.12 Indians NATIONAL INDIAN GAMING COMMISSION, DEPARTMENT OF THE INTERIOR HUMAN SERVICES MINIMUM TECHNICAL STANDARDS FOR GAMING EQUIPMENT USED WITH THE PLAY... gaming system? This section provides standards for downloading on a Class II gaming system. (a) Downloads...

  19. 25 CFR 547.7 - What are the minimum technical hardware standards applicable to Class II gaming systems?

    Science.gov (United States)

    2010-04-01

    ... applicable to Class II gaming systems? 547.7 Section 547.7 Indians NATIONAL INDIAN GAMING COMMISSION, DEPARTMENT OF THE INTERIOR HUMAN SERVICES MINIMUM TECHNICAL STANDARDS FOR GAMING EQUIPMENT USED WITH THE PLAY... gaming systems? (a) General requirements. (1) The Class II gaming system shall operate in compliance with...

  20. Pulsed corona demonstrator for semi-industrial scale air purification

    NARCIS (Netherlands)

    Beckers, F.J.C.M.; Hoeben, W.F.L.M.; Huiskamp, T.; Pemen, A.J.M.; Heesch, van E.J.M.

    2013-01-01

    Although pulsed corona technology for air purification is widely investigated by the lab experiments, large-scale application has yet to be proven. Industrial systems require large flow handling and thus, high corona power. An autonomous semi-industrial scale pilot wire-cylinder type corona reactor