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Sample records for purification systems ii

  1. The Borexino purification system

    Science.gov (United States)

    Benziger, Jay

    2014-05-01

    Purification of 278 tons of liquid scintillator and 889 tons of buffer shielding for the Borexino solar neutrino detector is performed with a system of combined distillation, water extraction, gas stripping and filtration. The purification system removed K, U and Th by distillation of the pseudocumene solvent and the PPO fluor. Noble gases, Rn, Kr and Ar were removed by gas stripping. Distillation was also employed to remove optical impurities and reduce the attenuation of scintillation light. The success of the purification system has facilitated the first time real time detection of low energy solar neutrinos.

  2. Increase of COP for heat transformer in water purification systems. Part II - Without increasing heat source temperature

    Energy Technology Data Exchange (ETDEWEB)

    Romero, R.J. [Centro de Investigacion en Ingenieria y Ciencias Aplicadas, Universidad Autonoma del Estado de Morelos, Av. Universidad 1001, Col. Chamilpa, C.P. 62209 Cuernavaca, Morelos (Mexico)]. E-mail: rosenberg@uaem.mx; Siqueiros, J. [Centro de Investigacion en Ingenieria y Ciencias Aplicadas, Universidad Autonoma del Estado de Morelos, Av. Universidad 1001, Col. Chamilpa, C.P. 62209 Cuernavaca, Morelos (Mexico); Huicochea, A. [Posgrado en Ingenieria y Ciencias Aplicadas, Universidad Autonoma del Estado de Morelos, Av. Universidad 1001, Col. Chamilpa, C.P. 62209 Cuernavaca, Morelos (Mexico)

    2007-04-15

    The integration of a water purification system allows a heat transformer to increase the actual coefficient of performance, by the reduction of the amount of heat supplied by unit of heat. A new defined COP called COP{sub WP} is proposed for the present system, which considers the fraction of heat recycled. Simulation with proven software compares the performance of the modeling of an absorption heat transformer for water purification (AHTWP) operating with water/lithium bromide, as working fluid-absorbent pair. Plots of enthalpy-based coefficients of performance (COP{sub ET}) and water purification coefficient of performance (COP{sub WP}) are shown against absorber temperature for several thermodynamic operating conditions. The results showed that the proposed (AHTWP) system is capable of increasing the original value of COP{sub ET} up to 1.6 times its original value by recycling energy from a water purification system. The proposed COP{sub WP} allows increments for COP values from any experimental data for water purification or for any other distillation system integrated to a heat transformer, regardless of actual COP{sub A} value or working fluid-absorbent pair.

  3. Water Purification Systems

    Science.gov (United States)

    1994-01-01

    Clearwater Pool Technologies employs NASA-developed silver/copper ionization to purify turtle and dolphin tanks, cooling towers, spas, water recycling systems, etc. The pool purifier consists of a microcomputer to monitor water conditions, a pair of metallic electrodes, and a rheostat controller. Ions are generated by passing a low voltage current through the electrodes; the silver ions kill the bacteria, and the copper ions kill algae. This technology has found broad application because it offers an alternative to chemical disinfectants. It was originally developed to purify water on Apollo spacecraft. Caribbean Clear has been using NASA's silver ionization technology for water purification for more than a decade. Two new products incorporate advancements of the basic technology. One is the AquaKing, a system designed for areas with no source of acceptable drinking water. Another is the Caribbean Clear Controller, designed for commercial pool and water park applications where sanitizing is combined with feedback control of pH and an oxidizer, chlorine or bromine. The technology was originally developed to purify water on Apollo spacecraft.

  4. The SELEX Air Purification System

    Science.gov (United States)

    2010-01-07

    REPORT Final Report for the SELEX Air Purification System 14. ABSTRACT 16. SECURITY CLASSIFICATION OF: A new air purification technology ( SELEX ) was...developed and demonstrated. The SELEX system utilizes an array of electrospray wick aerosol sources for particle ionization and an electrostatic...precipitator for particle collection. The particle ionization process does not produce ozone and the SELEX technology provides a unique combination of

  5. Reverse osmosis water purification system

    Science.gov (United States)

    Ahlstrom, H. G.; Hames, P. S.; Menninger, F. J.

    1986-01-01

    A reverse osmosis water purification system, which uses a programmable controller (PC) as the control system, was designed and built to maintain the cleanliness and level of water for various systems of a 64-m antenna. The installation operates with other equipment of the antenna at the Goldstone Deep Space Communication Complex. The reverse osmosis system was designed to be fully automatic; with the PC, many complex sequential and timed logic networks were easily implemented and are modified. The PC monitors water levels, pressures, flows, control panel requests, and set points on analog meters; with this information various processes are initiated, monitored, modified, halted, or eliminated as required by the equipment being supplied pure water.

  6. Staphylococcal micrococcins. II. Isolation, purification and identification.

    Science.gov (United States)

    Breiter, J; Metz, H; Grigo, J

    1975-08-01

    Seven strains belonging to the Micrococcaceae family and excreting substances with antibiotic activity, were grown in submerged cultures on technical scale for isolation, purification and identification of biologically active compounds. Two basic substances were isolated and classified to the micrococcin antibiotics family. The naturally occurring mixture of micrococcin M1 and M3 was called micrococcin M. This antibiotic has the formula C48H50O11N12S6 and a molecular weight of about 1160, melting point 221--224 degrees C, and optical rotation [a]20/D = + 66.6. Other antibiotically active substances produced by seven investigated strains were identified as micrococcin M or as separate compounds. Comparison with previously described micrococcin and micrococcin P has been made.

  7. Solid State Air Purification System Project

    Data.gov (United States)

    National Aeronautics and Space Administration — The purpose of this proposed research is to develop a new air purification system based on a liquid membrane, capable of purifying carbon dioxide from air in a far...

  8. 21 CFR 876.5665 - Water purification system for hemodialysis.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Water purification system for hemodialysis. 876... SERVICES (CONTINUED) MEDICAL DEVICES GASTROENTEROLOGY-UROLOGY DEVICES Therapeutic Devices § 876.5665 Water purification system for hemodialysis. (a) Identification. A water purification system for hemodialysis is...

  9. Recombinant allergen Lol p II: expression, purification and characterization.

    Science.gov (United States)

    Tamborini, E; Brandazza, A; De Lalla, C; Musco, G; Siccardi, A G; Arosio, P; Sidoli, A

    1995-05-01

    Pollen from perennial rye grass (Lolium perenne) is a major cause of type I allergies worldwide. It contains complex mixtures of proteins, among which Lol p II is a major allergen. Previously, we have reported the cloning and sequencing of Lol p II and its expression in fusion with the heavy chain of human ferritin as carrier polypeptide (Sidoli et al., 1993, J. biol. Chem. 268, 21819-21825). Here, we describe the expression, purification and characterization of a recombinant Lol p II overproduced as a non-fusion protein in the periplasm of E. coli. The recombinant allergen was expressed in high yields and was easily purified in milligram amounts. It competed with the natural Lol p II for binding to specific IgE, and it induced allergic responses in skin prick tests, indicating to be immunologically analogous to the natural protein. Biochemical analyses indicate that recombinant Lol p II is a highly stable and soluble monomeric molecule which behaves like a small globular protein.

  10. Breathing Air Purification for Hyperbaric Purposes, Part II

    Directory of Open Access Journals (Sweden)

    Woźniak Arkadiusz

    2015-03-01

    Full Text Available Determining the efficiency of breathing air purification for hyperbaric purposes with the use of filtration systems is of a crucial importance. However, when the Polish Navy took samples of breathing air from their own filtration plant for quality purposes, these were found to not meet the required standard. The identification of this problem imposed the need to undertake actions aimed at the elimination of the identified disruptions in the process of breathing air production, with the objective of assuring its proper quality. This study presents the results of the initial tests on the air supply sources utilised by the Polish Navy, which were carried out for the purpose of setting a proper direction of future works and implementing corrective measures in order to optimise the breathing air production process. The obtained test results will be used in a subsequent publication devoted to the assessment of the level of efficiency of air purification with the use of a multifaceted approach consisting in the utilisation of various types of air supply sources and different configurations of purification systems.

  11. Intein-mediated purification system: mechanism and applications

    Institute of Scientific and Technical Information of China (English)

    Sarra setrerrahmane; Shuhua Tan

    2013-01-01

    The incorporation of self-cleaving protein elements into a variety of fusion-based purification systems; has been an important development in the area of recombinant protein purification. The self-cleaving capability of these tags has recently been combined with additional purification tags to generate novel and convenient protein purification methods. This review elucidates the properties of intein, the mechanism of the intein-based protein splicing and the progress of intein-based protein purification procedures, and recent advances in the applications of intein.

  12. Automated Water-Purification System

    Science.gov (United States)

    Ahlstrom, Harlow G.; Hames, Peter S.; Menninger, Fredrick J.

    1988-01-01

    Reverse-osmosis system operates and maintains itself with minimal human attention, using programmable controller. In purifier, membranes surround hollow cores through which clean product water flows out of reverse-osmosis unit. No chemical reactions or phase changes involved. Reject water, in which dissolved solids concentrated, emerges from outer membrane material on same side water entered. Flow controls maintain ratio of 50 percent product water and 50 percent reject water. Membranes expected to last from 3 to 15 years.

  13. A scintillator purification system for the Borexino solar neutrino detector

    Science.gov (United States)

    Benziger, J.; Cadonati, L.; Calaprice, F.; Chen, M.; Corsi, A.; Dalnoki-Veress, F.; Fernholz, R.; Ford, R.; Galbiati, C.; Goretti, A.; Harding, E.; Ianni, Aldo; Ianni, Andrea; Kidner, S.; Leung, M.; Loeser, F.; McCarty, K.; McKinsey, D.; Nelson, A.; Pocar, A.; Salvo, C.; Schimizzi, D.; Shutt, T.; Sonnenschein, A.

    2008-03-01

    Purification of the 278 tons of liquid scintillator and 889 tons of buffer shielding for the Borexino solar neutrino detector is performed with a system that combines distillation, water extraction, gas stripping, and filtration. This paper describes the principles of operation, design, and construction of that purification system, and reviews the requirements and methods to achieve system cleanliness and leak-tightness.

  14. Methane monooxygenase from Methylosinus trichosporium OB3b. Purification and properties of a three-component system with high specific activity from a type II methanotroph.

    Science.gov (United States)

    Fox, B G; Froland, W A; Dege, J E; Lipscomb, J D

    1989-06-15

    Methane monooxygenase has been purified from the Type II methanotroph Methylosinus trichosporium OB3b. As observed for methane monooxygenase isolated from Type I methanotrophs, three protein components are required: a 39.7-kDa NADH reductase containing 1 mol each of FAD and a [2Fe-2S] cluster, a 15.8-kDa protein factor termed component B that contains no metals or cofactors, and a 245-kDa hydroxylase which appears to contain an oxo- or hydroxo-bridged binuclear iron cluster. Through the use of stabilizing reagents, the hydroxylase is obtained in high yield and exhibits a specific activity 8-25-fold greater than reported for previous preparations. The component B and reductase exhibit 1.5- and 4-fold greater specific activity, respectively. Quantitation of the hydroxylase oxo-bridged cluster using EPR and Mössbauer spectroscopies reveals that the highest specific activity preparations (approximately 1700 nmol/min/mg) contain approximately 2 clusters/mol. In contrast, hydroxylase preparations exhibiting a wide range of specific activities below 500 nmol/min/mg contain approximately 1 cluster/mol on average. Efficient turnover coupled to NADH oxidation requires all three protein components. However, both alkanes and alkenes are hydroxylated by the chemically reduced hydroxylase under single turnover conditions in the absence of component B and the reductase. Neither of these components catalyzes hydroxylation individually nor do they significantly affect the yield of hydroxylated product from the chemically reduced hydroxylase. Hydroxylase reduced only to the mixed valent [Fe(II).Fe(III)] state is unreactive toward O2 and yields little hydroxylated product on single turnover. This suggests that the catalytically active species is the fully reduced form. The data presented here provide the first evidence based on catalysis that the site of the monooxygenation reaction is located on the hydroxylase. It thus appears likely that the oxo-bridged iron cluster is capable of

  15. Efficient and versatile one-step affinity purification of in vivo biotinylated proteins: Expression, characterization and structure analysis of recombinant human glutamate carboxypeptidase II

    Energy Technology Data Exchange (ETDEWEB)

    Tykvart, J.; Sacha, P.; Barinka, C.; Knedlik, T.; Starkova, J.; Lubkowski, J.; Konvalinka, J. (Gilead); (NCI); (Czech Academy)

    2012-02-07

    Affinity purification is a useful approach for purification of recombinant proteins. Eukaryotic expression systems have become more frequently used at the expense of prokaryotic systems since they afford recombinant eukaryotic proteins with post-translational modifications similar or identical to the native ones. Here, we present a one-step affinity purification set-up suitable for the purification of secreted proteins. The set-up is based on the interaction between biotin and mutated streptavidin. Drosophila Schneider 2 cells are chosen as the expression host, and a biotin acceptor peptide is used as an affinity tag. This tag is biotinylated by Escherichia coli biotin-protein ligase in vivo. We determined that localization of the ligase within the ER led to the most effective in vivo biotinylation of the secreted proteins. We optimized a protocol for large-scale expression and purification of AviTEV-tagged recombinant human glutamate carboxypeptidase II (Avi-GCPII) with milligram yields per liter of culture. We also determined the 3D structure of Avi-GCPII by X-ray crystallography and compared the enzymatic characteristics of the protein to those of its non-tagged variant. These experiments confirmed that AviTEV tag does not affect the biophysical properties of its fused partner. Purification approach, developed here, provides not only a sufficient amount of highly homogenous protein but also specifically and effectively biotinylates a target protein and thus enables its subsequent visualization or immobilization.

  16. Purification of DNA polymerase II stimulatory factor I, a yeast single-stranded DNA-binding protein.

    OpenAIRE

    1990-01-01

    Incidental to the purification of yeast DNA polymerase II was the observation that various chromatographic fractions contained activities that stimulated synthesis by this polymerase. In this paper we report the purification and initial characterization of one such factor, stimulatory factor I (SFI). SFI, which is associated with an apparent complex of three polypeptides of 66, 37, and 13.5 kDa, binds preferentially to single-stranded DNA, possibly explaining its ability to stimulate DNA poly...

  17. Production and characterization of murine monoclonal anti-human DNase II antibodies, and their use for immunoaffinity purification of DNase II from human liver and urine.

    Science.gov (United States)

    Nakajima, Tamiko; Yasuda, Toshihiro; Takeshita, Haruo; Mori, Shinjiro; Mogi, Kouichi; Kaneko, Yasushi; Nakazato, Emiko; Kishi, Koichiro

    2002-04-15

    Four murine monoclonal anti-human deoxyribonuclease II (DNase II) antibodies were obtained from BALB/c mice immunized with human DNase II purified from human liver. Both single radial enzyme diffusion (SRED) and DNA-cast polyacrylamide gel electrophoresis (DNA-cast PAGE) were very useful for obtaining the DNase II-specific antibodies. All of the antibodies showed specific inhibition of human DNase II enzyme activity and specific immunostaining of the 32-kDa enzyme band, which is one of the three non-identical subunits of human DNase II molecule separated by sodium dodecyl sulfate (SDS)-PAGE followed by blotting on a transfer membrane. A formyl-cellulofine resin conjugated with each antibody specifically adsorbed and efficiently desorbed the active DNase II enzyme. Insertion of the immunoaffinity step in our purification procedure made the purification of human DNase II easier, faster and more effective than the conventional procedure.

  18. Affinity chromatography purification of angiotensin II reactor using photoactivable biotinylated probes

    Energy Technology Data Exchange (ETDEWEB)

    Marie, J.; Seyer, R.; Lombard, C.; Desarnaud, F.; Aumelas, A.; Jard, A.; Bonnafous, J.C. (Centre CNRS-INSERM de Pharmacologie-Endocrinologie, Montpellier (France))

    1990-09-25

    The authors have developed biotinylated photoactivable probes that are suitable for covalent labeling of angiotensin II (AII) receptors and the subsequent purification of covalent complexes through immobilized avidin or streptavidin. One of these probes, biotin-NH(CH{sub 2}){sub 2}SS(CH{sub 2}){sub 2}CO-(Ala{sup 1}, Phe(4N{sub 3}){sup 8})AII, which contains a cleavage disulfide bridge in its spacer arm and which displays, in its radioiodinated form, very high affinity for AII receptors (K{sub d}{approximately}1 nM), proved to be suitable for indirect affinity chromatography of rate liver receptor with facilitated recovery from avidin gels by use of reducing agents. This constituted the central step of an efficient partial purification scheme involving hydroxylapatite chromatography, streptavidin chromatography, and thiopropyl-Sepharose chromatography. SDS-PAGE analysis and autoradiography established the identity of the purified entity (molecular weight 65K) as the AII receptor. Possible ways of completing purification to homogeneity and extrapolation of the protocols to a preparative scale are discussed, as well as the potential contribution of our new probes to the study of the structural properties of angiotensin receptors.

  19. Affinity chromatography purification of angiotensin II receptor using photoactivable biotinylated probes.

    Science.gov (United States)

    Marie, J; Seyer, R; Lombard, C; Desarnaud, F; Aumelas, A; Jard, S; Bonnafous, J C

    1990-09-25

    We have developed biotinylated photoactivable probes that are suitable for covalent labeling of angiotensin II (AII) receptors and the subsequent purification of covalent complexes through immobilized avidin or streptavidin. One of these probes, biotin-NH(CH2)2SS(CH2)2CO-[Ala1,Phe(4N3)8]AII, which contains a cleavable disulfide bridge in its spacer arm and which displays, in its radioiodinated form, very high affinity for AII receptors (Kd approximately 1 nM), proved to be suitable for indirect affinity chromatography of rat liver receptor with facilitated recovery from avidin gels by use of reducing agents. This constituted the central step of an efficient partial purification scheme involving hydroxylapatite chromatography, streptavidin chromatography, and thiopropyl-Sepharose chromatography. SDS-PAGE analysis and autoradiography established the identity of the purified entity (molecular weight 65K) as the AII receptor. Possible ways of completing purification to homogeneity and extrapolation of the protocols to a preparative scale are discussed, as well as the potential contribution of our new probes to the study of the structural properties of angiotensin receptors.

  20. A Scintillator Purification Plant and Fluid Handling System for SNO+

    CERN Document Server

    Ford, Richard J

    2015-01-01

    A large capacity purification plant and fluid handling system has been constructed for the SNO+ neutrino and double-beta decay experiment, located 6800 feet underground at SNOLAB, Canada. SNO+ is a refurbishment of the SNO detector to fill the acrylic vessel with liquid scintillator based on Linear Alkylbenzene (LAB) and 2 g/L PPO, and also has a phase to load natural tellurium into the scintillator for a double-beta decay experiment with 130Te. The plant includes processes multi-stage dual-stream distillation, column water extraction, steam stripping, and functionalized silica gel adsorption columns. The plant also includes systems for preparing the scintillator with PPO and metal-loading the scintillator for double-beta decay exposure. We review the basis of design, the purification principles, specifications for the plant, and the construction and installations. The construction and commissioning status is updated.

  1. The effect of water purification systems on fluoride content of drinking water

    OpenAIRE

    Prabhakar A; Raju O; Kurthukoti A; Vishwas T

    2008-01-01

    Objective: The purpose of the present study was to determine the effect of different water purification systems on the fluoride content of drinking water and to compare the efficacy of these water purification systems in reducing the fluoride content. Materials and Methods: Five different water purification systems were tested in this study. They were reverse osmosis, distillation, activated carbon, Reviva ® , and candle filter. The water samples in the study were of two types, viz, bo...

  2. Two-phase aqueous micellar systems: an alternative method for protein purification

    Directory of Open Access Journals (Sweden)

    Rangel-Yagui C. O.

    2004-01-01

    Full Text Available Two-phase aqueous micellar systems can be exploited in separation science for the extraction/purification of desired biomolecules. This article reviews recent experimental and theoretical work by Blankschtein and co-workers on the use of two-phase aqueous micellar systems for the separation of hydrophilic proteins. The experimental partitioning behavior of the enzyme glucose-6-phosphate dehydrogenase (G6PD in two-phase aqueous micellar systems is also reviewed and new results are presented. Specifically, we discuss very recent work on the purification of G6PD using: i a two-phase aqueous micellar system composed of the nonionic surfactant n-decyl tetra(ethylene oxide (C10E4, and (ii a two-phase aqueous mixed micellar system composed of C10E4 and the cationic surfactant decyltrimethylammonium bromide (C10TAB. Our results indicate that the two-phase aqueous mixed (C10E4/C10TAB micellar system can improve significantly the partitioning behavior of G6PD relative to that observed in the two-phase aqueous C10E4 micellar system.

  3. New research on bioregenerative air/water purification systems

    Science.gov (United States)

    Johnson, Anne H.; Ellender, R. D.; Watkins, Paul J.

    1991-01-01

    For the past several years, air and water purification systems have been developed and used. This technology is based on the combined activities of plants and microorganisms as they function in a natural environment. More recently, researchers have begun to address the problems associated with indoor air pollution. Various common houseplants are currently being evaluated for their abilities to reduce concentrations of volatile organic compounds (VOCS) such as formaldehyde and benzene. With development of the Space Exploration Initiative, missions will increase in duration, and problems with resupply necessitates implementation of regenerative technology. Aspects of bioregenerative technology have been included in a habitat known as the BioHome. The ultimate goal is to use this technology in conjunction with physicochemical systems for air and water purification within closed systems. This study continued the risk assessment of bioregenerative technology with emphasis on biological hazards. In an effort to evaluate the risk for human infection, analyses were directed at enumeration of fecal streptococci and enteric viruses with the BioHome waste water treatment system.

  4. Circulation and Purification in the LUX-ZEPLIN System Test

    Science.gov (United States)

    Alsum, Shaun; Lz Collaboration

    2016-03-01

    LZ is a dark-matter direct detection experiment whose detector is a two-phase TPC using approximately seven tons of active xenon as its scintillator. The xenon must have few electronegative impurities to ensure sufficient electron transport through the drift region. The LZ purification system is being prototyped in the LZ system test, a test platform located at SLAC using about 100kg of Xenon, which consists of gas circulation through a SAES getter. We utilize a dual-phase and a gas-phase heat exchanger to reduce needed cooling power. To achieve this circulation we employ an all metal seal triple diaphragm pump, also prototyped in the System Test. This talk will present early results from the system test as well as some baseline LZ designs. The LUX-ZEPLIN dark matter direct detection experiment.

  5. Development of an automated system for isolation and purification of humic substances

    NARCIS (Netherlands)

    Zomeren, van A.; Weij-Zuiver, van der E.; Comans, R.N.J.

    2008-01-01

    Characterization of humic substances (HS) in environmental samples generally involves labor-intensive and time-consuming isolation and purification procedures. In this paper, the development of an automated system for HS isolation and purification is described. The novelty of the developed system li

  6. Development of an automated system for isolation and purification of humic substances

    NARCIS (Netherlands)

    Zomeren, van A.; Weij-Zuiver, van der E.; Comans, R.N.J.

    2008-01-01

    Characterization of humic substances (HS) in environmental samples generally involves labor-intensive and time-consuming isolation and purification procedures. In this paper, the development of an automated system for HS isolation and purification is described. The novelty of the developed system

  7. Purification and Characterization of the Manganese(II) Oxidizing Protein from Erythrobacter sp. SD-21

    Science.gov (United States)

    Nakama, K. R.; Lien, A.; Johnson, H. A.

    2013-12-01

    The manganese(II) oxidizing protein (Mop) found in the alpha-proteobacterium Erythrobacter sp. SD-21 catalyzes the formation of insoluble Mn(III/IV) oxides from soluble Mn(II). These Mn(III/IV) oxides formed are one of the strongest naturally occurring oxides, next to oxygen, and can be used to adsorb and oxidize toxic chemicals from the surrounding environment. Because of the beneficial use in the treatment of contaminated sources, the mechanism and biochemical properties of this novel enzyme are being studied. Due to low expression levels in the native host strain, purification of Mop has been problematic. To overcome this problem the gene encoding Mop, mopA, was cloned from the native host into a C-terminal histidine tag vector and expressed in Escherichia coli cells. Affinity chromatography under denaturing conditions have been applied in attempts to purify an active Mop. Western blots have confirmed that the protein is being expressed and is at the expected size of 250 kDa. Preliminary characterization on crude extract containing Mop has shown a Km and vmax value of 2453 uM and 0.025 uM min-1, respectively. Heme and pyrroloquinoline quinone can stimulate Mn(II) oxidizing activity, but hydrogen peroxide does not affect activity, despite the sequence similarity to animal heme peroxidase proteins. Research has been shown that calcium is essential for Mop activity. Purifying an active Mn(II) oxidizing protein will allow for a better understanding behind the enigmatic process of Mn(II) oxidation.

  8. DEVELOPMENT OF A NON-NOBLE METAL HYDROGEN PURIFICATION SYSTEM

    Energy Technology Data Exchange (ETDEWEB)

    Korinko, P; Kyle Brinkman, K; Thad Adams, T; George Rawls, G

    2008-11-25

    Development of advanced hydrogen separation membranes in support of hydrogen production processes such as coal gasification and as front end gas purifiers for fuel cell based system is paramount to the successful implementation of a national hydrogen economy. Current generation metallic hydrogen separation membranes are based on Pd-alloys. Although the technology has proven successful, at issue is the high cost of palladium. Evaluation of non-noble metal based dense metallic separation membranes is currently receiving national and international attention. The focus of the reported work was to develop a scaled reactor with a VNi-Ti alloy membrane to replace a production Pd-alloy tube-type purification/diffuser system.

  9. Expression, purification and characterization of enoyl-ACP reductase II, FabK, from Porphyromonas gingivalis

    Energy Technology Data Exchange (ETDEWEB)

    Hevener, Kirk E.; Mehboob, Shahila; Boci, Teuta; Truong, Kent; Santarsiero, Bernard D.; Johnson, Michael E. (UIC)

    2012-10-25

    The rapid rise in bacterial drug resistance coupled with the low number of novel antimicrobial compounds in the discovery pipeline has led to a critical situation requiring the expedient discovery and characterization of new antimicrobial drug targets. Enzymes in the bacterial fatty acid synthesis pathway, FAS-II, are distinct from their mammalian counterparts, FAS-I, in terms of both structure and mechanism. As such, they represent attractive targets for the design of novel antimicrobial compounds. Enoyl-acyl carrier protein reductase II, FabK, is a key, rate-limiting enzyme in the FAS-II pathway for several bacterial pathogens. The organism, Porphyromonas gingivalis, is a causative agent of chronic periodontitis that affects up to 25% of the US population and incurs a high national burden in terms of cost of treatment. P. gingivalis expresses FabK as the sole enoyl reductase enzyme in its FAS-II cycle, which makes this a particularly appealing target with potential for selective antimicrobial therapy. Herein we report the molecular cloning, expression, purification and characterization of the FabK enzyme from P. gingivalis, only the second organism from which this enzyme has been isolated. Characterization studies have shown that the enzyme is a flavoprotein, the reaction dependent upon FMN and NADPH and proceeding via a Ping-Pong Bi-Bi mechanism to reduce the enoyl substrate. A sensitive assay measuring the fluorescence decrease of NADPH as it is converted to NADP{sup +} during the reaction has been optimized for high-throughput screening. Finally, protein crystallization conditions have been identified which led to protein crystals that diffract x-rays to high resolution.

  10. THE WATER PURIFICATION SYSTEM OF MACHINE-BUILDING COMPLEX FROM OIL-PRODUCTS

    Directory of Open Access Journals (Sweden)

    A. S. Panasugin

    2005-01-01

    Full Text Available The developed system of purification of sewage water from petroleum products allows to provide efficiency of purification up to the norms of PDK, and if necessary the devices can be used as a separate modules or their combinations.

  11. Purification and characterization of a class II α-Mannosidase from Moringa oleifera seed kernels.

    Science.gov (United States)

    Tejavath, Kiran Kumar; Nadimpalli, Siva Kumar

    2014-10-01

    α-Mannosidase (EC. 3.2.1.114) belonging to class II glycosyl hydrolase family 38 was purified from Moringa oleifera seeds to apparent homogeneity by conventional protein purification methods followed by affinity chromatography on Con A Sepharose and size exclusion chromatography. The purified enzyme is a glycoprotein with 9.3 % carbohydrate and exhibited a native molecular mass of 240 kDa, comprising two heterogeneous subunits with molecular masses of 66 kDa (α-larger subunit) and 55 kDa (β-smaller subunit). Among both the subunits only larger subunit stained for carbohydrate with periodic acid Schiff's staining. The optimum temperature and pH for purified enzyme was 50 °C and pH 5.0, respectively. The enzyme was stable within the pH range of 3.0-7.0. The enzyme was inhibited by EDTA, Hg(2+), Ag(2+), and Cu(2+). The activity lost by EDTA was completely regained by addition of Zn(2+). The purified enzyme was characterized in terms of the kinetic parameters K m (1.6 mM) and V max (2.2 U/mg) using para-nitrophenyl-α-D-mannopyranoside as substrate. The enzyme was very strongly inhibited by swainsonine (SW) at 1 μM concentration a class II α-Mannosidase inhibitor, but not by deoxymannojirimycin (DMNJ). Chemical modification studies revealed involvement of tryptophan at active site. The inhibition by SW and requirement of the Zn(2+) as a metal ion suggested that the enzyme belongs to class II α-Mannosidase.

  12. The controllability analysis of the purification system for heavy water reactors

    Energy Technology Data Exchange (ETDEWEB)

    Lee, K. D.; Cho, B. H.; Shin, C. H.; Kim, S. H. [KEPRI, Taejon (Korea, Republic of); Lee, Y. K.; Kim, K. U. [KHNP, Kyungju (Korea, Republic of)

    2001-10-01

    The heavy water reactor such as Wolsung No.1 and No.2 has a purification system to purify the reactor coolant. The control system regulates the coolant temperature to protect the ion exchanger. After the fuel exchanges of operating plant, the increase of the coolant pressure makes the purification temperature control difficult. In this paper, the controllability of the control dynamics of the purification system was analysed and the optimal parameters were proposed. To reduce the effects of the flow disturbance, the feedforward control structure was proposed and analysed.

  13. The effect of water purification systems on fluoride content of drinking water

    Directory of Open Access Journals (Sweden)

    Prabhakar A

    2008-03-01

    Full Text Available Objective: The purpose of the present study was to determine the effect of different water purification systems on the fluoride content of drinking water and to compare the efficacy of these water purification systems in reducing the fluoride content. Materials and Methods: Five different water purification systems were tested in this study. They were reverse osmosis, distillation, activated carbon, Reviva ® , and candle filter. The water samples in the study were of two types, viz, borewell water and tap water, these being commonly used by the people of Davangere City, Karnataka. The samples were collected before and after purification, and fluoride analysis was done using fluoride ion-specific electrode. Results: The results showed that the systems based on reverse osmosis, viz, reverse osmosis system and Reviva ® showed maximum reduction in fluoride levels, the former proving to be more effective than the latter; followed by distillation and the activated carbon system, with the least reduction being brought about by candle filter. The amount of fluoride removed by the purification system varied between the system and from one source of water to the other. Interpretation and Conclusion: Considering the beneficial effects of fluoride on caries prevention; when drinking water is subjected to water purification systems that reduce fluoride significantly below the optimal level, fluoride supplementation may be necessary. The efficacy of systems based on reverse osmosis in reducing the fluoride content of water indicates their potential for use as defluoridation devices.

  14. The effect of water purification systems on fluoride content of drinking water.

    Science.gov (United States)

    Prabhakar, A R; Raju, O S; Kurthukoti, A J; Vishwas, T D

    2008-03-01

    The purpose of the present study was to determine the effect of different water purification systems on the fluoride content of drinking water and to compare the efficacy of these water purification systems in reducing the fluoride content. Five different water purification systems were tested in this study. They were reverse osmosis, distillation, activated carbon, Reviva , and candle filter. The water samples in the study were of two types, viz, borewell water and tap water, these being commonly used by the people of Davangere City, Karnataka. The samples were collected before and after purification, and fluoride analysis was done using fluoride ion-specific electrode. The results showed that the systems based on reverse osmosis, viz, reverse osmosis system and Reviva showed maximum reduction in fluoride levels, the former proving to be more effective than the latter; followed by distillation and the activated carbon system, with the least reduction being brought about by candle filter. The amount of fluoride removed by the purification system varied between the system and from one source of water to the other. Considering the beneficial effects of fluoride on caries prevention; when drinking water is subjected to water purification systems that reduce fluoride significantly below the optimal level, fluoride supplementation may be necessary. The efficacy of systems based on reverse osmosis in reducing the fluoride content of water indicates their potential for use as defluoridation devices.

  15. Undulative induction electron accelerator for the waste and natural water purification systems

    CERN Document Server

    Kulish, Victor V; Gubanov, I V

    2001-01-01

    The project analysis of Undulative Induction Accelerator (EH - accelerator) for the waste and natural water purification systems is accomplished. It is shown that the use of the four-channel design of induction block and the standard set of auxiliary equipment (developed earlier for the Linear Induction Accelerators - LINACs) allow to construct commercially promising purification systems. A quality analysis of the accelerator is done and the optimal parameters are chosen taking into account the specific sphere of its usage.

  16. Purification of replication factors using insect and mammalian cell expression systems.

    Science.gov (United States)

    Uno, Shuji; You, Zhiying; Masai, Hisao

    2012-06-01

    Purification of factors for DNA replication in an amount sufficient for detailed biochemical characterization is essential to elucidating its mechanisms. Insect cell expression systems are commonly used for purification of the factors proven to be difficult to deal with in bacteria. We describe first the detailed protocols for purification of mammalian Mcm complexes including the Mcm2/3/4/5/6/7 heterohexamer expressed in insect cells. We then describe a convenient and economical system in which large-sized proteins and multi-factor complexes can be transiently overexpressed in human 293T cells and be rapidly purified in a large quantity. We describe various expression vectors and detailed methods for transfection and purification of various replication factors which have been difficult to obtain in a sufficient amount in other systems. Availability of efficient methods to overproduce and purify the proteins that have been challenging would facilitate the enzymatic analyses of the processes of DNA replication.

  17. Practice and design of the self-purification system for heavy metals-bearing contaminants

    Institute of Scientific and Technical Information of China (English)

    Qian Guangren

    2008-01-01

    Many minerals in nature have self-purification capacity to hold and stabilize deleterious contaminants into their lattice structures,which can be used for treatment of heavy metals-bearing contaminants.Hydrotalcite Layer Double Hy-droxide(LDH),tobermorite Calcium Silicate Hydrate(CSH)and apatite are ubiquitous minerals in nature,having higher geochemical stability and potential for binding and stabilizing heavy metals.Based on the elucidation of crystal structure property and self-purification principles of the three minerals above,this article discussed how to design the self-purification system of heavy metal-bearing contaminants.

  18. Purification of liquid metal systems with sodium coolant from oxygen using getters

    Science.gov (United States)

    Kozlov, F. A.; Konovalov, M. A.; Sorokin, A. P.

    2016-05-01

    For increasing the safety and economic parameters of nuclear power stations (NPSs) with sodium coolant, it was decided to install all systems contacting radioactive sodium, including purification systems of circuit I, in the reactor vessel. The performance and capacity of cold traps (CTs) (conventional element of coolant purification systems) in these conditions are limited by their volume. It was proposed to use hot traps (HTs) in circuit I for coolant purification from oxygen. It was demonstrated that, at rated parameters of the installation when the temperature of the coolant streamlining the getter (gas absorber) is equal to 550°C, the hot trap can provide the required coolant purity. In shutdown modes at 250-300°C, the performance of the hot trap is reduced by four orders of magnitude. Possible HT operation regimes for shutdown modes and while reaching rated parameters were proposed and analyzed. Basic attention was paid to purification modes at power rise after commissioning and accidental contamination of the coolant when the initial oxygen concentration in it reached 25 mln-1. It was demonstrated that the efficiency of purification systems can be increased using HTs with the getter in the form of a foil or granules. The possibility of implementing the "fast purification" mode in which the coolant is purified simultaneously with passing over from the shutdown mode to the rated parameters was substantiated.

  19. Development of RAP Tag, a Novel Tagging System for Protein Detection and Purification.

    Science.gov (United States)

    Fujii, Yuki; Kaneko, Mika K; Ogasawara, Satoshi; Yamada, Shinji; Yanaka, Miyuki; Nakamura, Takuro; Saidoh, Noriko; Yoshida, Kanae; Honma, Ryusuke; Kato, Yukinari

    2017-04-01

    Affinity tag systems, possessing high affinity and specificity, are useful for protein detection and purification. The most suitable tag for a particular purpose should be selected from many available affinity tag systems. In this study, we developed a novel affinity tag called the "RAP tag" system, which comprises a mouse antirat podoplanin monoclonal antibody (clone PMab-2) and the RAP tag (DMVNPGLEDRIE). This system is useful not only for protein detection in Western blotting, flow cytometry, and sandwich enzyme-linked immunosorbent assay, but also for protein purification.

  20. NSLS-II RF SYSTEMS

    Energy Technology Data Exchange (ETDEWEB)

    Rose, J.; Gash, W.; Holub, B.; Kawashima, Y.; Ma, H.; Towne, N.; Yeddulla, M.

    2011-03-28

    The NSLS-II is a new third generation light source being constructed at Brookhaven Lab. The storage ring is optimized for low emittance by use of damping wigglers to reduce the emittance to below 1 nm-rad. The RF systems are designed to provide stable beam through tight RF phase and amplitude stability requirements.

  1. Design of the Helium Purifier for IHEP-ADS Helium Purification System

    CERN Document Server

    Jianqin, Zhang; Zhuo, Zhang; Rui, Ge

    2015-01-01

    Helium Purification System is an important sub-system in the Accelerator Driven Subcritical System of the Institute of High Energy Physics(IHEP ADS). The purifier is designed to work at the temperature of 77K. The purifier will work in a flow rate of 5g/s at 20MPa in continuous operation of 12 hours. The oil and moisture are removed by coalescing filters and a dryer, while nitrogen and oxygen are condensed by a phase separator and then adsorbed in several activated carbon adsorption cylinders. After purification, the purified helium has an impurity content of less than 5ppm.

  2. Identification of bacteria in drinking and purified water during the monitoring of a typical water purification system

    Directory of Open Access Journals (Sweden)

    Mazzola Priscila

    2002-08-01

    Full Text Available Abstract Background A typical purification system that provides purified water which meets ionic and organic chemical standards, must be protected from microbial proliferation to minimize cross-contamination for use in cleaning and preparations in pharmaceutical industries and in health environments. Methodology Samples of water were taken directly from the public distribution water tank at twelve different stages of a typical purification system were analyzed for the identification of isolated bacteria. Two miniature kits were used: (i identification system (api 20 NE, Bio-Mérieux for non-enteric and non-fermenting gram-negative rods; and (ii identification system (BBL crystal, Becton and Dickson for enteric and non-fermenting gram-negative rods. The efficiency of the chemical sanitizers used in the stages of the system, over the isolated and identified bacteria in the sampling water, was evaluated by the minimum inhibitory concentration (MIC method. Results The 78 isolated colonies were identified as the following bacteria genera: Pseudomonas, Flavobacterium and Acinetobacter. According to the miniature kits used in the identification, there was a prevalence of isolation of P. aeruginosa 32.05%, P. picketti (Ralstonia picketti 23.08%, P. vesiculares 12.82%,P. diminuta 11.54%, F. aureum 6.42%, P. fluorescens 5.13%, A. lwoffi 2.56%, P. putida 2.56%, P. alcaligenes 1.28%, P. paucimobilis 1.28%, and F. multivorum 1.28%. Conclusions We found that research was required for the identification of gram-negative non-fermenting bacteria, which were isolated from drinking water and water purification systems, since Pseudomonas genera represents opportunistic pathogens which disperse and adhere easily to surfaces, forming a biofilm which interferes with the cleaning and disinfection procedures in hospital and industrial environments.

  3. The importance of the ammonia purification process in ammonia-water absorption systems

    Energy Technology Data Exchange (ETDEWEB)

    Fernandez-Seara, Jose [Area de Maquinas y Motores Termicos, Escuela Tecnica Superior de Ingenieros Industriales, Universidad de Vigo, Campus Lagoas-Marcosende No. 9, 36200 Vigo (Spain)]. E-mail: jseara@uvigo.es; Sieres, Jaime [Area de Maquinas y Motores Termicos, Escuela Tecnica Superior de Ingenieros Industriales, Universidad de Vigo, Campus Lagoas-Marcosende No. 9, 36200 Vigo (Spain)

    2006-08-15

    Practical experience in working with ammonia-water absorption systems shows that the ammonia purification process is a crucial issue in order to obtain an efficient and reliable system. In this paper, the detrimental effects of the residual water content in the vapour refrigerant are described and quantified based on the system design variables that determine the effectiveness of the purification process. The study has been performed considering a single stage system with a distillation column with complete condensation. The ammonia purification effectiveness of the column is analysed in terms of the efficiencies in the stripping and rectifying sections and the reflux ratio. By varying the efficiencies from 0 to 1, systems with neither the rectifying nor stripping section, with either the rectifying or stripping section, or with both sections can be considered. The impact of the ammonia purification process on the absorption system performance is studied based on the column efficiencies and reflux ratio; and its effects on refrigerant concentration, system COP, system pressures and main system mass flow rates and concentrations are analysed. When the highest efficiency rectifying sections are used a combination of generation temperature and reflux ratio which leads to optimum COP values is found. The analysis covers different operating conditions with air and water cooled systems from refrigeration to air conditioning applications by changing the evaporation temperature. The importance of rectification in each kind of application is evaluated.

  4. Application of surface response analysis to the optimization of penicillin acylase purification in aqueous two-phase systems

    OpenAIRE

    2002-01-01

    Penicillin acylase purification from an Escherichia coli crude extract using PEG 3350 – sodium citrate aqueous two phase systems was optimized. An experimental design was used to evaluate the influence of PEG, sodium citrate and sodium chloride on the purification parameters. A central composite design was defined centred on the previously found conditions for highest purification from an osmotic shock extract. Mathematical models for the partition coefficient of protein and enzyme, balance o...

  5. Chemical resistance of the gram-negative bacteria to different sanitizers in a water purification system

    Directory of Open Access Journals (Sweden)

    Penna Thereza CV

    2006-08-01

    Full Text Available Abstract Background Purified water for pharmaceutical purposes must be free of microbial contamination and pyrogens. Even with the additional sanitary and disinfecting treatments applied to the system (sequential operational stages, Pseudomonas aeruginosa, Pseudomonas fluorescens, Pseudomonas alcaligenes, Pseudomonas picketti, Flavobacterium aureum, Acinetobacter lowffi and Pseudomonas diminuta were isolated and identified from a thirteen-stage purification system. To evaluate the efficacy of the chemical agents used in the disinfecting process along with those used to adjust chemical characteristics of the system, over the identified bacteria, the kinetic parameter of killing time (D-value necessary to inactivate 90% of the initial bioburden (decimal reduction time was experimentally determined. Methods Pseudomonas aeruginosa, Pseudomonas fluorescens, Pseudomonas alcaligenes, Pseudomonas picketti, Flavobacterium aureum, Acinetobacter lowffi and Pseudomonas diminuta were called in house (wild bacteria. Pseudomonas diminuta ATCC 11568, Pseudomonas alcaligenes INCQS , Pseudomonas aeruginosa ATCC 15442, Pseudomonas fluorescens ATCC 3178, Pseudomonas picketti ATCC 5031, Bacillus subtilis ATCC 937 and Escherichia coli ATCC 25922 were used as 'standard' bacteria to evaluate resistance at 25°C against either 0.5% citric acid, 0.5% hydrochloric acid, 70% ethanol, 0.5% sodium bisulfite, 0.4% sodium hydroxide, 0.5% sodium hypochlorite, or a mixture of 2.2% hydrogen peroxide (H2O2 and 0.45% peracetic acid. Results The efficacy of the sanitizers varied with concentration and contact time to reduce decimal logarithmic (log10 population (n cycles. To kill 90% of the initial population (or one log10 cycle, the necessary time (D-value was for P. aeruginosa into: (i 0.5% citric acid, D = 3.8 min; (ii 0.5% hydrochloric acid, D = 6.9 min; (iii 70% ethanol, D = 9.7 min; (iv 0.5% sodium bisulfite, D = 5.3 min; (v 0.4% sodium hydroxide, D = 14.2 min; (vi 0.5% sodium

  6. Preparation and Purification of 125I With Neutron Irradiated Xenon in a Vacuum Circular system

    Institute of Scientific and Technical Information of China (English)

    MIAOZeng-xing; LIYu-cheng; YUNing-wen; WUJie; XIANGXue-qin; ZHAOXiu-yan

    2003-01-01

    This paper describes the preparation and purification of 125I with neutron irradiated xenon in a vacuum circular system, which is specially designed with an irradiate chamber set inside of the reactor and three decay chambers set outside of the reactor. The xenon is filled in this system and recurrently circulates between the irradiate chamber and the decay chambers during the reactor is operating.

  7. Bromelain purification through unconventional aqueous two-phase system (PEG/ammonium sulphate).

    Science.gov (United States)

    Coelho, D F; Silveira, E; Pessoa Junior, A; Tambourgi, E B

    2013-02-01

    This paper focuses on the feasibility of unconventional aqueous two-phase systems for bromelain purification from pineapple processing waste. The main difference in comparison with conventional systems is the integration of the liquid-liquid extraction technique with fractional precipitation, which can decrease the protein content with no loss of biological activity by removing of unwanted molecules. The analysis of the results was based on the response surface methodology and revealed that the use of the desirability optimisation methodology (DOM) was necessary to achieve higher purification factor values and greater bromelain recovery. The use of DOM yielded an 11.80-fold purification factor and 66.38 % biological activity recovery using poly(ethylene glycol) (PEG) with a molar mass of 4,000, 10.86 % PEG concentration (m/m) and 36.21 % saturation of ammonium sulphate.

  8. MPS II drift chamber system

    Energy Technology Data Exchange (ETDEWEB)

    Platner, E.D.

    1982-01-01

    The MPS II detectors are narrow drift space chambers designed for high position resolution in a magnetic field and in a very high particle flux environment. Central to this implementation was the development of 3 multi-channel custom IC's and one multi-channel hybrid. The system is deadtimeless and requires no corrections on an anode-to-anode basis. Operational experience and relevance to ISABELLE detectors is discussed.

  9. Expression and purification of recombinant HTLV-I/-II linear epitopes antigen and its application for screening of suspected patients.

    Science.gov (United States)

    Faramarzi, Roxana; Dolatabadi, Samaneh

    2017-02-01

    The linear epitopes of gp46-I, gp46-II, gp21 and p19 are used in diagnosis of HTLV-I/-II infections. The aims of this study was to obtain high-level expression and purification of recombinant antigen (RA) containing these epitopes. Large-scale preparation of such antigen probably worths for diagnostic purpose. The synthetic DNA encoding RA was synthesized and over-expressed as soluble in Escherichia coli BL21 (DE3) cells. Expression and distribution of the His-GST-RA protein were evaluated using SDS-PAGE. The soluble RA was purified utilizing Ni-NTA agarose beads under native conditions and was concentrated by ultra filtration. Using 20 sera specimens from HTLV infected patients, the antigenicity of the purified protein was confirmed in ELISA and western blotting analysis. SDS-PAGE revealed that the purified protein was more than 90% pure. The final yield was approximately 25 mg per liter of culture medium. ELISA results showed that RA could specifically bind to anti-HTLV-I/-II antibodies in infected sera. RA could be a candidate for HTLV-I/-II screening and the strategy presented in this study could be used for easy production of this diagnostic protein.

  10. 21 CFR 884.6170 - Assisted reproduction water and water purification systems.

    Science.gov (United States)

    2010-04-01

    ... HUMAN SERVICES (CONTINUED) MEDICAL DEVICES OBSTETRICAL AND GYNECOLOGICAL DEVICES Assisted Reproduction Devices § 884.6170 Assisted reproduction water and water purification systems. (a) Identification... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Assisted reproduction water and water...

  11. Field Testing of a Small Water Purification System for Non-PRASA Rural Communities

    Science.gov (United States)

    Small, rural communities typically do not have adequate water purification systems to sustain their life quality and residents are exposed to pathogens present in drinking water. In Puerto Rico (PR), approximately 4% of the population does not have access to drinking water provi...

  12. Field Testing of a Small Water Purification System for Non-PRASA Rural Communities

    Science.gov (United States)

    Small, rural communities typically do not have adequate water purification systems to sustain their life quality and residents are exposed to pathogens present in drinking water. In Puerto Rico (PR), approximately 4% of the population does not have access to drinking water provi...

  13. THE INFLUENCE OF INDIVIDUAL FACTORS ON THE EFFECTIVENESS OF JUICE PURIFICATION IN THE PROCESS OF II SATURATION

    Directory of Open Access Journals (Sweden)

    V. A. Golybin

    2014-01-01

    Full Text Available Summary. The effect of reducing substances in the final stage of lime - carbon dioxide purification of raw juice is studied in the article. The presence of significant amounts of reducing substances in the juice of the I saturation increases chroma and calcium salts in the purified product. It is actual to apply additional techniques and methods of cleaning of production sugar-containing solutions at the final stage of lime -carbon dioxide cleaning - II saturation, that will increase the completeness of precipitation of organic and mineral non-sugars, improve the quality of the purified juice, increase the yield of white sugar and improve its quality. The effect filtroperlit as seed material for forming the structure of particles of calcium carbonate precipitate with a larger surface adsorption is studied. The effect of phosphate input for further improvement of the efficiency of adsorption in the juice purification process was also studied. The effect of flow of activated filtroperlit on II saturation filtration speed was studied. It was found out that the more non-sugars are present in the juice, the smaller electrokinetic potential has the surface sediment. Rational consumption of reagents depending on the quality of the feedstock is calculated. In the process of cleaning the juice of various technological quality, it is necessary to control the reagents flow. It was found out that for cleaning juice of satisfactory technological quality the flow of filtroperlit is 0.015 - 0.033% by weight of juice and 15% РО4 3- . When cleaning the juice obtained from sugar beet of poor quality, it is necessary to increase the filtroperlit flow up to 0.050% and phosphate up to 20 %. It is necessary to control permanently the main liming process, the maximum decomposition of reducing substances to obtain thermally stable juice.

  14. Purification and characterization of an insulin-like growth factor II variant from human plasma.

    Science.gov (United States)

    Hampton, B; Burgess, W H; Marshak, D R; Cullen, K J; Perdue, J F

    1989-11-15

    An insulin-like growth factor II variant (IGF-II variant) was purified from Cohn fraction IV1 of human plasma by ion exchange, gel filtration, and reversed-phase high pressure liquid chromatography. The amino-terminal sequence of the first 35 amino acid residues showed a replacement of Ser-29 of IGF-II with the tetrapeptide Arg-Leu-Pro-Gly of IGF-II variant. Peptides isolated and sequenced after digestion with endoproteinase Asp-N and endoproteinase Glu-C disclosed no differences with the sequence predicted from an IGF-II variant cDNA clone isolated by Jansen, M., van Shaik, F. M. A., van Tol, H., Van den Brande, J. L., and Sussenbach, J. S. (1985) FEBS Lett., 179, 243-246. The molecular ion of intact IGF-II variant was 7809.4 mass units, as measured by plasma desorption mass spectrometry. This is in close agreement with the molecular ion of 7812.8 mass units calculated from the determined sequence and indicates the entire amino acid sequence had been accounted for. Binding of IGF-II variant to purified insulin-like growth factor I (IGF-I) receptors demonstrated a 2-3-fold lower affinity for this receptor compared with IGF-I or IGF-II. The dissociation constants for IGF-I, IGF-II, and IGF-II variant are 0.23, 0.38, and 0.80 nM, respectively. In a growth assay, the concentration of IGF-II and IGF-II variant required to stimulate the half-maximal growth of MCF-7 cells was 4 and 13 nM, respectively. Finally, the amount of IGF-II variant that can be purified by this method constitutes approximately 25% of the total IGF-II isolated from Cohn fraction IV1 of human plasma.

  15. Hamiltonian purification

    Energy Technology Data Exchange (ETDEWEB)

    Orsucci, Davide [Scuola Normale Superiore, I-56126 Pisa (Italy); Burgarth, Daniel [Department of Mathematics, Aberystwyth University, Aberystwyth SY23 3BZ (United Kingdom); Facchi, Paolo; Pascazio, Saverio [Dipartimento di Fisica and MECENAS, Università di Bari, I-70126 Bari (Italy); INFN, Sezione di Bari, I-70126 Bari (Italy); Nakazato, Hiromichi; Yuasa, Kazuya [Department of Physics, Waseda University, Tokyo 169-8555 (Japan); Giovannetti, Vittorio [NEST, Scuola Normale Superiore and Istituto Nanoscienze-CNR, I-56126 Pisa (Italy)

    2015-12-15

    The problem of Hamiltonian purification introduced by Burgarth et al. [Nat. Commun. 5, 5173 (2014)] is formalized and discussed. Specifically, given a set of non-commuting Hamiltonians (h{sub 1}, …, h{sub m}) operating on a d-dimensional quantum system ℋ{sub d}, the problem consists in identifying a set of commuting Hamiltonians (H{sub 1}, …, H{sub m}) operating on a larger d{sub E}-dimensional system ℋ{sub d{sub E}} which embeds ℋ{sub d} as a proper subspace, such that h{sub j} = PH{sub j}P with P being the projection which allows one to recover ℋ{sub d} from ℋ{sub d{sub E}}. The notions of spanning-set purification and generator purification of an algebra are also introduced and optimal solutions for u(d) are provided.

  16. Purification of uranothorite solid solutions from polyphase systems

    Energy Technology Data Exchange (ETDEWEB)

    Clavier, Nicolas, E-mail: nicolas.clavier@icsm.fr [ICSM, UMR 5257 CEA/CNRS/UM2/ENSCM, Site de Marcoule – Bât. 426, BP 17171, 30207 Bagnols/Cèze cedex (France); Szenknect, Stéphanie; Costin, Dan Tiberiu; Mesbah, Adel; Ravaux, Johann [ICSM, UMR 5257 CEA/CNRS/UM2/ENSCM, Site de Marcoule – Bât. 426, BP 17171, 30207 Bagnols/Cèze cedex (France); Poinssot, Christophe [CEA/DEN/DRCP/DIR, Site de Marcoule – Bât. 400, BP 17171, 30207 Bagnols/Cèze cedex (France); Dacheux, Nicolas [ICSM, UMR 5257 CEA/CNRS/UM2/ENSCM, Site de Marcoule – Bât. 426, BP 17171, 30207 Bagnols/Cèze cedex (France)

    2013-10-15

    Graphical abstract: Display Omitted -- Highlights: •Purification of Th{sub 1−x}U{sub x}SiO{sub 4} uranothorites from oxide mixture was investigated. •Repetition of centrifugation steps was discarded due to poor recovery yields. •Successive washings in acid and basic media allowed the elimination of oxide secondary phases. •Structural and microstructural characterization of the purified samples was provided. -- Abstract: The mineral coffinite, nominally USiO{sub 4}, and associated Th{sub 1−x}U{sub x}SiO{sub 4} uranothorite solid solutions are of great interest from a geochemical point of view and in the case of the direct storage of spent nuclear fuels. Nevertheless, they clearly exhibit a lack in the evaluation of their thermodynamic data, mainly because of the difficulties linked with their preparation as pure phases. This paper thus presents physical and chemical methods aiming to separate uranothorite solid solutions from oxide additional phases such as amorphous SiO{sub 2} and nanometric crystallized Th{sub 1−y}U{sub y}O{sub 2}. The repetition of centrifugation steps envisaged in first place was rapidly dropped due to poor recovery yields, to the benefit of successive washings in acid then basic media. Under both static and dynamic flow rates (i.e. low or high rate of leachate renewal), ICP-AES (Inductively Coupled Plasma – Atomic Emission Spectroscopy) analyses revealed the systematic elimination of Th{sub 1−y}U{sub y}O{sub 2} in acid media and of SiO{sub 2} in basic media. Nevertheless, two successive steps were always needed to reach pure samples. On this basis, a first cycle performed in static conditions was chosen to eliminate the major part of the accessory phases while a second one, in dynamic conditions, allowed the elimination of the residual impurities. The complete purification of the samples was finally evidenced through the characterization of the samples by the means of PXRD (Powder X-Ray Diffraction), SEM (Scanning Electron

  17. Advanced Water Purification System for In Situ Resource Utilization Project

    Science.gov (United States)

    Anthony, Stephen M.

    2014-01-01

    A main goal in the field of In Situ Resource Utilization is to develop technologies that produce oxygen from regolith to provide consumables to an extratrrestrial outpost. The processes developed reduce metal oxides in the regolith to produce water, which is then electrolyzed to produce oxygen. Hydrochloric and hydrofluoric acids are byproducts of the reduction processes, which must be removed to meet electrolysis purity standards. We previously characterized Nation, a highly water selective polymeric proton-exchange membrane, as a filtrtion material to recover pure water from the contaminated solution. While the membranes successfully removed both acid contaminants, the removal efficiency of and water flow rate through the membranes were not sufficient to produce large volumes of electrolysis-grade water. In the present study, we investigated electrodialysis as a potential acid removable technique. Our studies have show a rapid and significant reduction in chloride and fluoride concentrations in the feed solution, while generating a relatively small volume of concentrated waste water. Electrodialysis has shown significant promise as the primary separation technique in ISRU water purification processes.

  18. Advanced Water Purification System for In Situ Resource Utilization

    Science.gov (United States)

    Anthony, Stephen M.; Jolley, Scott T.; Captain, James G.

    2013-01-01

    A main goal in the field of In Situ Resource Utilization is to develop technologies that produce oxygen from regolith to provide consumables to an extraterrestrial outpost. The processes developed reduce metal oxides in the regolith to produce water, which is then electrolyzed to produce oxygen. Hydrochloric and hydrofluoric acids are byproducts of the reduction processes, which must be removed to meet electrolysis purity standards. We previously characterized Nation, a highly water selective polymeric proton-exchange membrane, as a filtration material to recover pure water from the contaminated solution. While the membranes successfully removed both acid contaminants, the removal efficiency of and water flow rate through the membranes were not sufficient to produce large volumes of electrolysis-grade water. In the present study, we investigated electrodialysis as a potential acid removal technique. Our studies have shown a rapid and significant reduction in chloride and fluoride concentrations in the feed solution, while generating a relatively small volume of concentrated waste water. Electrodialysis has shown significant promise as the primary separation technique in ISRU water purification processes.

  19. Efficiency in removing pollutants by constructed wetland purification systems in Poland.

    Science.gov (United States)

    Samecka-Cymerman, A; Stepien, D; Kempers, A J

    2004-02-27

    The aim of this study was to compare the efficiency between Phragmites communis, Salix viminalis, and Populus canadensis in removing the heavy metals Al, Ba, Mn, Ni, Sr, V, Zn, Cd, Cu, and Pb and the eutrophying macroelements phosphate, nitrate, nitrite, ammonia, chloride, sulfate, Ca, Mg, K, and Fe from sewage in subsurface flow constructed wetlands in Poland. The effectiveness of the sewage treatment system was higher in summer compared to winter for the removal of (1) all heavy metals, phosphate (P) and mineral nitrogen (N) for all of species, (2) sulfates (S) for Phragmites and Salix, (3) iron (Fe) for Salix, and (4) chloride (Cl) for Salix and Populus. Analysis of variance indicated that there was no significant difference between the purifications systems in phytoremediation of Mn; so all species were equally effective (99%, prob. level 0.001). The Salix wetland system was most effective in purification of water and removal of macroelements (24-82% in summer, 10-80% in winter with Fe 97%), Cd (58-71%), V (100%), and Zn (84-92%). The Phragmites system was most effective in purification and removal of Al (81-97%), Ba (70-95%), Pb (64-81%), and Sr (24-51%), while in the case of Cu (49-60%) and Ni (55-67%) the Populus wetland system proved most effective. The outflowing water of the wetlands contained elements in amounts exceeding the admissible levels as established for unpolluted water both in winter and summer. Therefore the effectiveness of the observed phytoremediation systems in this study was not sufficient alone to remove these elements and can be considered as a supplemental tool in purification of sewage.

  20. Guide to the use of pond systems in South Africa for the purification of raw and partially treated sewage

    CSIR Research Space (South Africa)

    Meiring, PGJ

    1968-01-01

    Full Text Available Some of the effluents from the sewage purification systems covered by this report show quite marked seasonal variations. No assurance can be given that the effluents would meet promulgated standards from season to season. The effluent from...

  1. Argus II retinal prosthesis system: An update.

    Science.gov (United States)

    Rachitskaya, Aleksandra V; Yuan, Alex

    2016-09-01

    This review focuses on a description of the Argus II retinal prosthesis system (Argus II; Second Sight Medical Products, Sylmar, CA) that was approved for humanitarian use by the FDA in 2013 in patients with retinitis pigmentosa with bare or no light perception vision. The article describes the components of Argus II, the studies on the implant, and future directions.

  2. Performance Assessment of SOFC Systems Integrated with Bio-Ethanol Production and Purification Processes

    Directory of Open Access Journals (Sweden)

    Sumittra Charojrochkul

    2010-03-01

    Full Text Available The overall electrical efficiencies of the integrated systems of solid oxide fuel cell (SOFC and bio-ethanol production with purification processes at different heat integration levels were investigated. The simulation studies were based on the condition with zero net energy. It was found that the most suitable operating voltage is between 0.7 and 0.85 V and the operating temperature is in the range from 973 to 1173 K. For the effect of percent ethanol recovery, the optimum percent ethanol recovery is at 95%. The most efficient case is the system with full heat integration between SOFC and bio-ethanol production and purification processes with biogas reformed for producing extra hydrogen feed for SOFC which has the overall electrical efficiency = 36.17%. However more equipment such as reformer and heat exchangers are required and this leads to increased investment cost.

  3. Dialysate purification after introduction of automated hot water disinfection system to central dialysis fluid delivery system.

    Science.gov (United States)

    Ogawa, Tomonari; Matsuda, Akihiko; Yamaguchi, Yumiko; Sasaki, Yusuke; Kanayama, Yuki; Maeda, Tadaaki; Noiri, Chie; Hasegawa, Hajime; Matsumura, Osamu; Mitarai, Tetsuya

    2012-01-01

    Most dialysis clinics in Japan have mainly adopted the central dialysis fluid delivery system (CDDS) to provide constant treatment to many patients. Chemical disinfection is the major maintenance method of the CDDS. Our clinic introduced an automated hot water disinfection system that used the heat conduction effect to disinfect a reverse osmosis (RO) device and dialysis fluid supply equipment. Endotoxin level and the amount of viable bacteria often showed abnormal values before introduction of this system. After its introduction, weekly disinfection resulted in endotoxin levels and the amount of viable bacteria lower than measurement sensitivity. In hot water disinfection, water heated to 90°C in the RO tank flows into the dialysis fluid supply equipment. The maximum temperature inside the tank of the supply equipment is 86.3°C. (We confirmed that the temperature was maintained at 80°C or more for 10 minutes or more during the monitoring.) Dialysate purification was maintained even after introduction of the automated hot water disinfection system and the dialysate could be supplied stably by the CDDS. Therefore, this disinfection system might be very useful in terms of both cost and safety, and can be used for dialysis treatment of multiple patients.

  4. Final LDRD report :ultraviolet water purification systems for rural environments and mobile applications.

    Energy Technology Data Exchange (ETDEWEB)

    Banas, Michael Anthony; Crawford, Mary Hagerott; Ruby, Douglas Scott; Ross, Michael P.; Nelson, Jeffrey Scott; Allerman, Andrew Alan; Boucher, Ray

    2005-11-01

    We present the results of a one year LDRD program that has focused on evaluating the use of newly developed deep ultraviolet LEDs in water purification. We describe our development efforts that have produced an LED-based water exposure set-up and enumerate the advances that have been made in deep UV LED performance throughout the project. The results of E. coli inactivation with 270-295 nm LEDs are presented along with an assessment of the potential for applying deep ultraviolet LED-based water purification to mobile point-of-use applications as well as to rural and international environments where the benefits of photovoltaic-powered systems can be realized.

  5. Purification of dialysis water in the central dialysis fluid delivery system in Japan: a prospective observation study.

    Science.gov (United States)

    Uchino, Junji; Kawasaki, Tadayuki

    2009-01-01

    Whereas the main measure of dialysis fluid purity is endotoxin (ET) activity in Japan, it is the viability count in Western countries. Because of this difference, little information is available concerning dialysis fluid purity determined in terms of viability count in Japan. Under these circumstances a fact-finding investigation was planned and conducted concerning dialysis fluid purity to demonstrate the effectiveness of dialysis fluid purification measures. 93 medical institutions are equipped with the central dialysis fluid delivery system (CDDS) unique to Japan. Almost all medical institutions surveyed have achieved the purification level of ultrapure dialysis fluid after ETRF, but the methods of ETRF use and management widely vary with each institution so that early validation of the methods of evaluation of ET inhibition and system management is in urgent need. It is also important that simple universal microbial monitoring and purification procedures be diffused far and wide as suggested by the Purification Guidelines proposed by us.

  6. Isolation and purification of complex II from proteus mirabilis strain ATCC 29245

    Directory of Open Access Journals (Sweden)

    Khadija Shabbiri

    2010-10-01

    Full Text Available A respiratory complex was isolated from plasma membrane of pathogenic Proteus mirabilis strain ATCC 29245. It was identified as complex II consisting of succinate:quinone oxidoreductase (EC 1.3.5.1 containing single heme b. The complex II was purified by ion-exchange chromatography and gel filtration. The molecular weight of purified complex was 116.5 kDa and it was composed of three subunits with molecular weights of 19 kDa, 29 kDa and 68.5 kDa. The complex II contained 9.5 nmoles of cytochrome b per mg protein. Heme staining indicated that the 19 kDa subunit was cytochrome b. Its reduced form showed absorptions peaks at 557.0, 524.8 and 424.4 nm. The α-band was shifted from 557.0 nm to 556.8 nm in pyridine ferrohemochrome spectrum. The succinate: quinone oxidoreductase activity was found to be high in this microorganism.

  7. Design of the Helium Purifier for IHEP-ADS Helium Purification System

    OpenAIRE

    2015-01-01

    Helium Purification System is an important sub-system in the Accelerator Driven Subcritical System of the Institute of High Energy Physics(IHEP ADS). The purifier is designed to work at the temperature of 77K. The purifier will work in a flow rate of 5g/s at 20MPa in continuous operation of 12 hours. The oil and moisture are removed by coalescing filters and a dryer, while nitrogen and oxygen are condensed by a phase separator and then adsorbed in several activated carbon adsorption cylinders...

  8. Development of a new hydrogen purification system by using hydrogen absorbing alloy for generator cooling; Suiso kyuzo gokin riyo hatsudenkinai suiso jundo kojo system no kaihatsu

    Energy Technology Data Exchange (ETDEWEB)

    Haruki, N.; Sato, J.; Kogi, T.; Nishimura, Y. [Kansai Electric Power Co., Inc., Osaka (Japan); Takeda, H. [Japan Steel works Ltd., Tokyo (Japan)] Fujita, T. [Mitsubishi Electric Corp., Tokyo (Japan)

    1997-05-20

    Hydrogen absorbing alloys have a number of useful functions, such as energy conversion, hydrogen storage and purification. As an application to separation and purification of hydrogen, we have developed a new hydrogen purification system by using a hydrogen absorbing alloy for generator cooling. For demonstration testing with an actual machine, a hydrogen recovery and purification device using 120kg of alloy was manufactured and installed on No.5 turbine-synchronous generator at Himeji No.2 power station. This device is designed to improve the purity of the hydrogen gas in generator containing impurities such as nitrogen and oxygen. The test results tell that the purity of the hydrogen gas in the generator can be enhanced from 98% to 99.9% and maintained at this level under continuous operation. An application of the hydrogen purification system is expected to decrease the generator`s windage loss, resulting higher generator efficiency. 2 refs., 18 figs.

  9. Biofilm bacterial communities in urban drinking water distribution systems transporting waters with different purification strategies.

    Science.gov (United States)

    Wu, Huiting; Zhang, Jingxu; Mi, Zilong; Xie, Shuguang; Chen, Chao; Zhang, Xiaojian

    2015-02-01

    Biofilm formation in drinking water distribution systems (DWDS) has many adverse consequences. Knowledge of microbial community structure of DWDS biofilm can aid in the design of an effective control strategy. However, biofilm bacterial community in real DWDS and the impact of drinking water purification strategy remain unclear. The present study investigated the composition and diversity of biofilm bacterial community in real DWDSs transporting waters with different purification strategies (conventional treatment and integrated treatment). High-throughput Illumina MiSeq sequencing analysis illustrated a large shift in the diversity and structure of biofilm bacterial community in real DWDS. Proteobacteria, Firmicutes, Bacteroidetes, Actinobacteria, Nitrospirae, and Cyanobacteria were the major components of biofilm bacterial community. Proteobacteria (mainly Alphaproteobacteria, Betaproteobacteria, and Gammaproteobacteria) predominated in each DWDS biofilm, but the compositions of the dominant proteobacterial classes and genera and their proportions varied among biofilm samples. Drinking water purification strategy could shape DWDS biofilm bacterial community. Moreover, Pearson's correlation analysis indicated that Actinobacteria was positively correlated with the levels of total alkalinity and dissolved organic carbon in tap water, while Firmicutes had a significant positive correlation with nitrite nitrogen.

  10. NSLS-II Radio Frequency Systems

    Energy Technology Data Exchange (ETDEWEB)

    Rose J.; Gao F.; Goel, A.; Holub, B.; Kulpin, J.; Marques, C.; Yeddulla, M.

    2015-05-03

    The National Synchrotron Light Source II is a 3 GeV X-ray user facility commissioned in 2014. The NSLS-II RF system consists of the master oscillator, digital low level RF controllers, linac, booster and storage ring RF sub-systems, as well as a supporting cryogenic system. Here we will report on RF commissioning and early operation experience of the system.

  11. Purification of polyclonal antibodies from Cohn fraction II + III, skim milk, and whey by affinity chromatography using a hexamer peptide ligand.

    Science.gov (United States)

    Menegatti, Stefano; Naik, Amith D; Gurgel, Patrick V; Carbonell, Ruben G

    2012-11-01

    HWRGWV, a peptide that binds specifically to the Fc fragment of human immunoglobulin G (IgG), was used for the purification of IgG from Cohn fraction II + III of human plasma and from bovine skim milk and whey. The concentration of sodium chloride and sodium caprylate in the binding buffer as well as the pH of the elution buffer were optimized to achieve high IgG yield and purity. Under optimized conditions, IgG was recovered from plasma fractions with yield and purity up to 84% and 95%, respectively. IgG was also purified from skim milk with 74% yield and 92% purity and from whey with 85% yield and 93% purity. Purification experiments were also performed with Protein A resin and the results were found to be similar to those obtained with the peptide adsorbent.

  12. A cost-effective ELP-intein coupling system for recombinant protein purification from plant production platform.

    Directory of Open Access Journals (Sweden)

    Li Tian

    Full Text Available BACKGROUND: Plant bioreactor offers an efficient and economical system for large-scale production of recombinant proteins. However, high cost and difficulty in scaling-up of downstream purification of the target protein, particularly the common involvement of affinity chromatography and protease in the purification process, has hampered its industrial scale application, therefore a cost-effective and easily scale-up purification method is highly desirable for further development of plant bioreactor. METHODOLOGY/PRINCIPAL FINDINGS: To tackle this problem, we investigated the ELP-intein coupling system for purification of recombinant proteins expressed in transgenic plants using a plant lectin (PAL with anti-tumor bioactivity as example target protein and rice seeds as production platform. Results showed that ELP-intein-PAL (EiP fusion protein formed novel irregular ER-derived protein bodies in endosperm cells by retention of endogenous prolamins. The fusion protein was partially self-cleaved in vivo, but only self-cleaved PAL protein was detected in total seed protein sample and deposited in protein storage vacuoles (PSV. The in vivo uncleaved EiP protein was accumulated up to 2-4.2% of the total seed protein. The target PAL protein could be purified by the ELP-intein system efficiently without using complicated instruments and expensive chemicals, and the yield of pure PAL protein by the current method was up to 1.1 mg/g total seed protein. CONCLUSION/SIGNIFICANCE: This study successfully demonstrated the purification of an example recombinant protein from rice seeds by the ELP-intein system. The whole purification procedure can be easily scaled up for industrial production, providing the first evidence on applying the ELP-intein coupling system to achieve cost-effective purification of recombinant proteins expressed in plant bioreactors and its possible application in industry.

  13. Polonium purification

    Energy Technology Data Exchange (ETDEWEB)

    Baker, J.D.

    1996-09-01

    Three processes for the purification of {sup 210}Po from irradiated bismuth targets are described. Safety equipment includes shielded hotcells for the initial separation from other activation products, gloveboxes for handling the volatile and highly toxic materials, and provisions for ventilation. All chemical separations must be performed under vacuum or in inerted systems. Two of the processes require large amounts of electricity; the third requires vessels made from exotic materials.

  14. PURIFICATION OF COBALT ANOLYTE USING THE NOVEL SOLVENT EXTRACTION SYSTEM

    Institute of Scientific and Technical Information of China (English)

    Y.F. Shen; W.Y. Xue; W. Y. Niu

    2003-01-01

    In present research, a novel extractant system (D2EHPA + naphthenic acid +pyridine-ester) was used to purify cobalt anolyte and a simulated industrial produc-tion were carried out. This novel extraction system can extract Cu and/or Ni againstCo from chloride medium solutions at pH range of 2.5-4.5. About 2g/l nickel and0.2g/l copper were removed from the cobalt chloride anolyte containing about 100g/lcobalt and 200g/l chloride ions respectively, the raffinate contains nickel and copperless than 0.03g/l and 0. 0003g/l respectively and can be used to electrolyze high-puritycobalt. About 5.5t cobalt anolyte was purified in the simulation industrial experimentand kilogram quantities of cobalt of 99.98% purity and about 95% recovery have beenproduced.

  15. DOMESTIC WASTEWATER PURIFICATION IN UPFLOW BIOFILM SYSTEM WITH DIFFUSED AERATION

    Directory of Open Access Journals (Sweden)

    A.Mesdaghinia

    1984-08-01

    Full Text Available The objective of this research was to conduct a bench scale study of fixed activated sludge treating domestic sewage. Two different units employing diffused aeration with plastic and aluminum media were studied in four separate phases. Data indicated that the system could produce a high quality effluent without any requirements for sludge recycling through the system. Suspended solids concentrations of 3-6 mg/1, BOD5 concentrations of 4-12 mg/1 and COD concentrations of 35-45 mg/1 were found in the effluent with wastewater retentions ranging from 3-15 hours, whereas an indication of nitrification was observed in higher detention periods. As far as the type of media was concerned, the plastic and aluminum media did not differ significantly once the microbes had grown on the media.

  16. Water purification through vacuum system; Purificacion de agua bajo vacio

    Energy Technology Data Exchange (ETDEWEB)

    Armenta-Deu, C.

    2004-07-01

    Fresh water production through vacuum systems are today a reasonable option at a much lower cost than tray conventional units, also based on evaporation-condensation process. The use of simple devices such as vacuum ejectors allows to reduce pressure down to 5 kPa at a very low cost, only 7. The requirement of having a constant water flow to reduce pressure has been easily solved using a close circuit and a low power pump which is powered by solar energy. The energy cost has been reduced dramatically, as the system operates at a very reduced temperature, 45 degree Celsius, and even as low as 35 degree Celsius, what causes a much lower energy requirement. The results obtained during the tests have shown that is possible to save up to 230 kJ per litre of fresh water, and up to 40 W per l/h. The system is fully compatible with thermal solar collectors of low temperature, and can be electrically powered by a solar panel of low power. (Author)

  17. Increase of COP for heat transformer in water purification systems. Pt. 2 - Without increasing heat source temperature

    Energy Technology Data Exchange (ETDEWEB)

    Romero, R.J.; Siqueiros, J. [Centro de Investigacion en Ingenieria y Ciencias Aplicadas, Universidad Autonoma del Estado de Morelos, Av. Universidad 1001, Col. Chamilpa, C.P. 62209 Cuernavaca, Morelos (Mexico); Huicochea, A. [Posgrado en Ingenieria y Ciencias Aplicadas, Universidad Autonoma del Estado de Morelos, Av. Universidad 1001, Col. Chamilpa, C.P. 62209 Cuernavaca, Morelos (Mexico)

    2007-04-15

    The integration of a water purification system allows a heat transformer to increase the actual coefficient of performance, by the reduction of the amount of heat supplied by unit of heat. A new defined COP called COP{sub WP} is proposed for the present system, which considers the fraction of heat recycled. Simulation with proven software compares the performance of the modeling of an absorption heat transformer for water purification (AHTWP) operating with water/lithium bromide, as working fluid-absorbent pair. Plots of enthalpy-based coefficients of performance (COP{sub ET}) and water purification coefficient of performance (COP{sub WP}) are shown against absorber temperature for several thermodynamic operating conditions. The results showed that the proposed (AHTWP) system is capable of increasing the original value of COP{sub ET} up to 1.6 times its original value by recycling energy from a water purification system. The proposed COP{sub WP} allows increments for COP values from any experimental data for water purification or for any other distillation system integrated to a heat transformer, regardless of actual COP{sub A} value or working fluid-absorbent pair. (author)

  18. Membrane-based systems for carbon capture and hydrogen purification

    Energy Technology Data Exchange (ETDEWEB)

    Berchtold, Kathryn A [Los Alamos National Laboratory

    2010-11-24

    This presentation describes the activities being conducted at Los Alamos National Laboratory to develop carbon capture technologies for power systems. This work is aimed at continued development and demonstration of a membrane based pre- and post-combustion carbon capture technology and separation schemes. Our primary work entails the development and demonstration of an innovative membrane technology for pre-combustion capture of carbon dioxide that operates over a broad range of conditions relevant to the power industry while meeting the US DOE's Carbon Sequestration Program goals of 90% CO{sub 2} capture at less than a 10% increase in the cost of energy services. Separating and capturing carbon dioxide from mixed gas streams is a first and critical step in carbon sequestration. To be technically and economically viable, a successful separation method must be applicable to industrially relevant gas streams at realistic temperatures and pressures as well as be compatible with large gas volumes. Our project team is developing polymer membranes based on polybenzimidazole (PBI) chemistries that can purify hydrogen and capture CO{sub 2} at industrially relevant temperatures. Our primary objectives are to develop and demonstrate polymer-based membrane chemistries, structures, deployment platforms, and sealing technologies that achieve the critical combination of high selectivity, high permeability, chemical stability, and mechanical stability all at elevated temperatures (> 150 C) and packaged in a scalable, economically viable, high area density system amenable to incorporation into an advanced Integrated Gasification Combined-Cycle (IGCC) plant for pre-combustion CO{sub 2} capture. Stability requirements are focused on tolerance to the primary synthesis gas components and impurities at various locations in the IGCC process. Since the process stream compositions and conditions (temperature and pressure) vary throughout the IGCC process, the project is focused on

  19. Hydrogen Purification and Recycling for an Integrated Oxygen Recovery System Architecture

    Science.gov (United States)

    Abney, Morgan B.; Greenwood, Zachary; Wall, Terry; Nur, Mononita; Wheeler, Richard R., Jr.; Preston, Joshua; Molter, Trent

    2016-01-01

    The United States Atmosphere Revitalization life support system on the International Space Station (ISS) performs several services for the crew including oxygen generation, trace contaminant control, carbon dioxide (CO2) removal, and oxygen recovery. Oxygen recovery is performed using a Sabatier reactor developed by Hamilton Sundstrand, wherein CO2 is reduced with hydrogen in a catalytic reactor to produce methane and water. The water product is purified in the Water Purification Assembly and recycled to the Oxygen Generation Assembly (OGA) to provide O2 to the crew. This architecture results in a theoretical maximum oxygen recovery from CO2 of approx.54% due to the loss of reactant hydrogen in Sabatier-produced methane that is currently vented outside of ISS. Plasma Pyrolysis Assembly (PPA) technology, developed by Umpqua Research Company, provides the capability to further close the Atmosphere Revitalization oxygen loop by recovering hydrogen from Sabatier-produced methane. A key aspect of this technology approach is the need to purify the hydrogen from the PPA product stream which includes acetylene, unreacted methane and byproduct water and carbon monoxide. In 2015, four sub-scale hydrogen separation systems were delivered to NASA for evaluation. These included two electrolysis single-cell hydrogen purification cell stacks developed by Sustainable Innovations, LLC, a sorbent-based hydrogen purification unit using microwave power for sorbent regeneration developed by Umpqua Research Company, and a LaNi4.6Sn0.4 metal hydride produced by Hydrogen Consultants, Inc. Here we report the results of these evaluations to-date, discuss potential architecture options, and propose future work.

  20. Hydrogen Purification and Recycling for an Integrated Oxygen Recovery System Architecture

    Science.gov (United States)

    Abney, Morgan B.; Greenwood, Zachary; Wall, Terry; Miller, Lee; Wheeler, Ray

    2016-01-01

    The United States Atmosphere Revitalization life support system on the International Space Station (ISS) performs several services for the crew including oxygen generation, trace contaminant control, carbon dioxide (CO2) removal, and oxygen recovery. Oxygen recovery is performed using a Sabatier reactor developed by Hamilton Sundstrand, wherein CO2 is reduced with hydrogen in a catalytic reactor to produce methane and water. The water product is purified in the Water Purification Assembly and recycled to the Oxygen Generation Assembly (OGA) to provide O2 to the crew. This architecture results in a theoretical maximum oxygen recovery from CO2 of approximately 54% due to the loss of reactant hydrogen in Sabatier-produced methane that is currently vented outside of ISS. Plasma Methane Pyrolysis technology (PPA), developed by Umpqua Research Company, provides the capability to further close the Atmosphere Revitalization oxygen loop by recovering hydrogen from Sabatier-produced methane. A key aspect of this technology approach is to purify the hydrogen from the PPA product stream which includes acetylene, unreacted methane and byproduct water and carbon monoxide. In 2015, four sub-scale hydrogen separation systems were delivered to NASA for evaluation. These included two electrolysis single-cell hydrogen purification cell stacks developed by Sustainable Innovations, LLC, a sorbent-based hydrogen purification unit using microwave power for sorbent regeneration developed by Umpqua Research Company, and a LaNi4.6Sn0.4 metal hydride produced by Hydrogen Consultants, Inc. Here we report the results of these evaluations, discuss potential architecture options, and propose future work.

  1. LHC II system sensitivity to magnetic fluids

    CERN Document Server

    Cotae, Vlad

    2005-01-01

    Experiments have been designed to reveal the influences of ferrofluid treatment and static magnetic field exposure on the photosynthetic system II, where the light harvesting complex (LHC II) controls the ratio chlorophyll a/ chlorophyll b (revealing, indirectly, the photosynthesis rate). Spectrophotometric measurement of chlorophyll content revealed different influences for relatively low ferrofluid concentrations (10-30 mul/l) in comparison to higher concentrations (70-100 mul/l). The overlapped effect of the static magnetic field shaped better the stimulatory ferrofluid action on LHC II system in young poppy plantlets.

  2. Water Collection Purification System: Identifying CF Capabilities and Requirements, and Assessing off-the-Shelf Purification Systems

    Science.gov (United States)

    2006-08-01

    media Ohio Pure Water Co Sand and silica dioxide of different grain sizes Birm media filter Ohio Pure Water Co Specific resin for iron when water does...Terminator filters Ohio Pure Water Co Same than Birm filter but with air injection system to add oxygen in the mixture Nitrate filter Ohio Pure Water Co...media 1,000-4,000 Birm media filter 1,000-2,000 Manganese greensand filter 1,000-2,700 Terminator filters 800-1,000 Nitrate filter

  3. Analysis of exosome purification methods using a model liposome system and tunable-resistive pulse sensing

    Science.gov (United States)

    Lane, Rebecca E.; Korbie, Darren; Anderson, Will; Vaidyanathan, Ramanathan; Trau, Matt

    2015-01-01

    Exosomes are vesicles which have garnered interest due to their diagnostic and therapeutic potential. Isolation of pure yields of exosomes from complex biological fluids whilst preserving their physical characteristics is critical for downstream applications. In this study, we use 100 nm-liposomes from 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and cholesterol as a model system as a model system to assess the effect of exosome isolation protocols on vesicle recovery and size distribution using a single-particle analysis method. We demonstrate that liposome size distribution and ζ-potential are comparable to extracted exosomes, making them an ideal model for comparison studies. Four different purification protocols were evaluated, with liposomes robustly isolated by three of them. Recovered yields varied and liposome size distribution was unaltered during processing, suggesting that these protocols do not induce particle aggregation. This leads us to conclude that the size distribution profile and characteristics of vesicles are stably maintained during processing and purification, suggesting that reports detailing how exosomes derived from tumour cells differ in size to those from normal cells are reporting a real phenomenon. However, we hypothesize that larger particles present in most purified exosome samples represent co-purified contaminating non-exosome debris. These isolation techniques are therefore likely nonspecific and may co-isolate non-exosome material of similar physical properties.

  4. Purification of C-phycocyanin from Spirulina platensis in aqueous two-phase systems using an experimental design

    Directory of Open Access Journals (Sweden)

    Francine Silva Antelo

    2015-02-01

    Full Text Available C-phycocyanin from Spirulina platensis was purified in aqueous two-phase systems (ATPS of polyethylene glycol (PEG/potassium phosphate, varying the molar mass of the PEG. Results using a full factorial design showed that an increase in the concentration of salt and decrease in the concentration of PEG caused an increment in the purification factor for all the ATPS studied. Optimization of the conditions of the purification was studied using a central composite rotatable design for each molar mass of PEG. The ATPS composed of 7% (w/w PEG 1500 or 4% (w/w PEG 8000 (g/gmol and 23 or 22.5% (w/w of phosphate resulted a purification factor of 1.6-fold for C-phycocyanin, with total and 57% recovery, respectively. Process conditions were optimized for the purification factor for the system with PEG 1500. The ATPS with 4% (w/w PEG 4000 or 4% (w/w PEG 6000 and 21% (w/w phosphate resulted purification factors of 2.1 and 2.2-fold, recovering 100% and 73.5%, respectively of C-phycocyanin in the top phase.

  5. A vector system for ABC transporter-mediated secretion and purification of recombinant proteins in Pseudomonas species.

    Science.gov (United States)

    Ryu, Jaewook; Lee, Ukjin; Park, Jiye; Yoo, Do-Hyun; Ahn, Jung Hoon

    2015-03-01

    Pseudomonas fluorescens is an efficient platform for recombinant protein production. P. fluorescens has an ABC transporter secreting endogenous thermostable lipase (TliA) and protease, which can be exploited to transport recombinant proteins across the cell membrane. In this study, the expression vector pDART was constructed by inserting tliDEF, genes encoding the ABC transporter, along with the construct of the lipase ABC transporter recognition domain (LARD), into pDSK519, a widely used shuttle vector. When the gene for the target protein was inserted into the vector, the C-terminally fused LARD allowed it to be secreted through the ABC transporter into the extracellular medium. After secretion of the fused target protein, the LARD containing a hydrophobic C terminus enabled its purification through hydrophobic interaction chromatography (HIC) using a methyl-Sepharose column. Alkaline phosphatase (AP) and green fluorescent protein (GFP) were used to validate the expression, export, and purification of target proteins by the pDART system. Both proteins were secreted into the extracellular medium in P. fluorescens. In particular, AP was secreted in several Pseudomonas species with its enzymatic activity in extracellular media. Furthermore, purification of the target protein using HIC yielded some degree of AP and GFP purification, where AP was purified to almost a single product. The pDART system will provide greater convenience for the secretory production and purification of recombinant proteins in Gram-negative bacteria, such as Pseudomonas species.

  6. Expression, purification, and bioactivity of GST-fused v-Src from a bacterial expression system

    Institute of Scientific and Technical Information of China (English)

    GONG Xing-guo; JI Jing; XIE Jie; ZHOU Yuan; ZHANG Jun-yan; ZHONG Wen-tao

    2006-01-01

    v-Src is a non-receptor protein tyrosine kinase involved in many signal transduction pathways and closely related to the activation and development of cancers. We present herethe expression, purification, and bioactivity of a GST (glutathione S-transferase)-fused v-Src from a bacterial expression system. Different culture conditions were examined in an isopropyl β-D-thiogalactopyranoside (IPTG)-regulated expression, and the fused protein was purified using GSH (glutathione) affinity chromatography. ELISA (enzyme-linked immunosorbent assay) was employed to determine the phosphorylation kinase activity of the GST-fused v-Src. This strategy seems to be more promising than the insect cell system or other eukaryotic systems employed in earlier Src expression.

  7. Particle Systems and PDEs II

    CERN Document Server

    Soares, Ana

    2015-01-01

    This book focuses on mathematical problems concerning different applications in physics, engineering, chemistry and biology. It covers topics ranging from interacting particle systems to partial differential equations (PDEs), statistical mechanics and dynamical systems. The purpose of the second meeting on Particle Systems and PDEs was to bring together renowned researchers working actively in the respective fields, to discuss their topics of expertise and to present recent scientific results in both areas. Further, the meeting was intended to present the subject of interacting particle systems, its roots in and impacts on the field of physics, and its relation with PDEs to a vast and varied public, including young researchers. The book also includes the notes from two mini-courses presented at the conference, allowing readers who are less familiar with these areas of mathematics to more easily approach them. The contributions will be of interest to mathematicians, theoretical physicists and other researchers...

  8. Handbook of power systems II

    Energy Technology Data Exchange (ETDEWEB)

    Rebennack, Steffen [Colorado School of Mines, Golden, CO (United States). Div. of Economics and Business; Pardalos, Panos M. [Florida Univ., Gainesville, FL (United States). Dept. of Industrial and Systems Engineering; Pereira, Mario V.F. [Centro Empresarial Rio Praia de Botafogo, Rio de Janeiro (Brazil); Iliadis, Niko A. (eds.) [EnerCoRD, Athens (Greece)

    2010-07-01

    Energy is one of the world's most challenging problems, and power systems are an important aspect of energy related issues. This handbook contains state-of-the-art contributions on power systems modeling and optimization. The book is separated into two volumes with six sections, which cover the most important areas of energy systems. The first volume covers the topics operations planning and expansion planning while the second volume focuses on transmission and distribution modeling, forecasting in energy, energy auctions and markets, as well as risk management. The contributions are authored by recognized specialists in their fields and consist in either state-of-the-art reviews or examinations of state-of-the-art developments. The articles are not purely theoretical, but instead also discuss specific applications in power systems. (orig.)

  9. Hyperentanglement purification for two-photon six-qubit quantum systems

    Science.gov (United States)

    Wang, Guan-Yu; Liu, Qian; Deng, Fu-Guo

    2016-09-01

    Recently, two-photon six-qubit hyperentangled states were produced in experiment and they can improve the channel capacity of quantum communication largely. Here we present a scheme for the hyperentanglement purification of nonlocal two-photon systems in three degrees of freedom (DOFs), including the polarization, the first-longitudinal-momentum, and the second-longitudinal-momentum DOFs. Our hyperentanglement purification protocol (hyper-EPP) is constructed with two steps resorting to parity-check quantum nondemolition measurement on the three DOFs and swap gates, respectively. With these two steps, the bit-flip errors in the three DOFs can be corrected efficiently. Also, we show that using swap gates is a universal method for hyper-EPP in the polarization DOF and multiple-longitudinal-momentum DOFs. The implementation of our hyper-EPP is assisted by nitrogen-vacancy centers in optical microcavities, which could be achieved with current techniques. It is useful for long-distance high-capacity quantum communication with two-photon six-qubit hyperentanglement.

  10. BEPC II wire scanner system

    Institute of Scientific and Technical Information of China (English)

    SUI Yan-Feng; WANG Lin; ZHAO Ying; YUE Jun-Hui; LI Xiao-Ping; CAO Jian-She; MA Li

    2010-01-01

    To monitor the beam profile at the end of the linac non-destructively,a wire scanner as a new diagnostic instrument was designed,manufactured and installed in 2007.Since then,several measurements have been carried out using this device.This paper describes the whole system of the wire scanner and the testing results.

  11. Effect of charcoal on water purification

    OpenAIRE

    Suzuki, Hirotaka; Kawahigashi, Tatsuo

    2014-01-01

    [Abstract] A natural basin system purifies water through self-purification, but the water pollution load of a river might exceed its self-purification capacity. Charcoal, which is used for other uses aside from heating, such as air purification, was evaluated experimentally for water quality purification. The experiment described herein is based on simple water quality measurements. Some experimentally obtained results are discussed.

  12. Nanostructured Catalytic Reactors for Air Purification Project

    Data.gov (United States)

    National Aeronautics and Space Administration — This SBIR Phase II project proposes the development of lightweight compact nanostructured catalytic reactors for air purification from toxic gaseous organic...

  13. Development of concept for concurrent biocide generation and water system purification. [with application to Skylab water tanks

    Science.gov (United States)

    1974-01-01

    An attempt was made to construct an electrochemical system, using iodine, for water purification in Skylab. Data cover measurements of iodine production rates, effect of electrode size and geometry on iodine production rates, and feasibility of using stainless steels as reference electrodes.

  14. Fusion tags for protein solubility, purification and immunogenicity in Escherichia coli: The novel Fh8 system

    Directory of Open Access Journals (Sweden)

    Sofia eCosta

    2014-02-01

    Full Text Available Proteins are now widely produced in diverse microbial cell factories. The Escherichia coli is still the dominant host for recombinant protein production but, as a bacterial cell, it also has its issues: the aggregation of foreign proteins into insoluble inclusion bodies is perhaps the main limiting factor of the E. coli expression system. Conversely, E. coli benefits of cost, ease of use and scale make it essential to design new approaches directed for improved recombinant protein production in this host cell.With the aid of genetic and protein engineering novel tailored-made strategies can be designed to suit user or process requirements. Gene fusion technology has been widely used for the improvement of soluble protein production and/or purification in E. coli, and for increasing peptide’s immunogenicity as well. New fusion partners are constantly emerging and complementing the traditional solutions, as for instance, the Fh8 fusion tag that has been recently studied and ranked among the best solubility enhancer partners. In this review, we provide an overview of current strategies to improve recombinant protein production in E. coli, including the key factors for successful protein production, highlighting soluble protein production, and a comprehensive summary of the latest available and traditionally-used gene fusion technologies. A special emphasis is given to the recently discovered Fh8 fusion system that can be used for soluble protein production, purification and immunogenicity in E. coli. The number of existing fusion tags will probably increase in the next few years, and efforts should be taken to better understand how fusion tags act in E. coli. This knowledge will undoubtedly drive the development of new tailored-made tools for protein production in this bacterial system.

  15. Application of mobile blood purification system in the treatment of acute renal failure dog model in the field environment

    Directory of Open Access Journals (Sweden)

    Zhi-min ZHANG

    2014-01-01

    Full Text Available Objective To evaluate the stability, safety and efficacy of mobile blood purification system in the treatment of acute renal failure dog model in the field environment. Methods The acute renal failure model was established in 4 dogs by bilateral nephrectomy, which was thereafter treated with the mobile blood purification system. The evaluation of functional index of the mobile blood purification system was performed after a short-time (2 hours and conventional (4 hours dialysis treatment. Results The mobile blood purification system ran stably in the field environment at a blood flow of 150-180ml/min, a dialysate velocity of 2000ml/h, a replacement fluid velocity of 2000ml/h, and ultrafiltration rate of 100-200ml/h. All the functions of alarming system worked well, including static upper limit alarm of ultrafiltration pressure (>100 mmHg, upper limit alarm of ambulatory arterial pressure (>400mmHg, upper limit alarm of ambulatory venous pressure (>400mmHg, bubble alarm of vascular access, bubble alarm during the infusion of solutions, pressure alarm at the substitution pump segment and blood leaking alarm. The vital signs of the 4 dogs with acute renal failure kept stable during the treatment. After the treatment, a remarkable decrease was seen in the levels of serum urea nitrogen, creatinine and serum potassium (P0.05. Conclusions The mobile blood purification system runs normally even in a field environment. It is a flexible and portable device with a great performance in safety and stability in the treatment of acute renal failure. DOI: 10.11855/j.issn.0577-7402.2013.12.15

  16. Purification of papain by metal affinity partitioning in aqueous two-phase polyethylene glycol/sodium sulfate systems.

    Science.gov (United States)

    Jiang, Zhi-Guo; Zhang, Hai-De; Wang, Wei-Tao

    2015-05-01

    A simple and inexpensive aqueous two-phase affinity partitioning system using metal ligands was introduced to improve the selectivity of commercial papain extraction. Polyethylene glycol 4000 was first activated using epichlorohydrin, then it was covalently linked to iminodiacetic acid. Finally, the specific metal ligand Cu(2+) was attached to the polyethylene glycol-iminodiacetic acid. The chelated Cu(2+) content was measured by atomic absorption spectrometry as 0.88 mol/mol (polyethylene glycol). The effects on the purification at different conditions, including polyethylene glycol molecular weight (2000, 4000, and 6000), concentration of phase-forming components (polyethylene glycol 12-20% w/w and sodium sulfate 12-20%, w/w), metal ligand type, and concentration, system pH and the commercial papain loading on papain extraction, were systematically studied. Under optimum conditions of the system, i.e. 18% w/w sodium sulfate, 18% w/w polyethylene glycol 4000, 1% w/w polyethylene glycol-iminodiacetic acid-Cu(2+) and pH 7, a maximum yield of 90.3% and a degree of purification of 3.6-fold were obtained. Compared to aqueous two phase extraction without ligands, affinity partitioning was found to be an effective technique for the purification of commercial papain with higher extraction efficiency and degree of purification.

  17. [Selection of winter plant species for wetlands constructed as sewage treatment systems and evaluation of their wastewater purification potentials].

    Science.gov (United States)

    Chen, Yong-hua; Wu, Xiao-fu; Chen, Ming-li; Jiang, Li-juan; Li, Ke-lin; Lei, Dian; Wang, Hai-bin

    2010-08-01

    In order to establish an evaluation system for selection of winter wetland plants possessing high wastewater purification potentials in subtropics areas, designed sewage treatment experiments were carried out by introducing into the constructed wetlands 25 species of winter wetland plants. Cluster analysis was performed by including harmful environment-resistant enzyme and substrate enzyme activities into the commonly applied plant screening and assessment indexes system. The obtained results indicated that there were significant differences among the tested winter plants in their root length and vigor, leaf malonaldehyde (MDA), biomass, average nitrogen and phosphorus concentration and uptake, and urease and phosphoric acid enzyme activities in the root areas. Based on the established evaluation system, the tested plants were clustered into 3 groups. The plants in the 1st group possessing high purification potentials are Oenanthe javanica, Brassicacapestris, Juncus effusu, Saxifragaceae, Iris pseudoacorus, Osmanthus fragrans and Iris ensata; those in the 2nd group possessing moderate purification potentials are Brassica oleracea var acephala, Calendula officinalis, Aucuba japonica, Ligustrum lucidu, Beta vulgaris, Rhododendron simsii and Ilex latifolia; and those in the 3rd group with low purification potentials are Brassica oleracea var acephala, Calistephus chinensis, Rosa chinensis, Antirrhinums, Liriope palatyphylla, Zephyranthes candida, Fatshedera lizei, Petunia hybrida, Ilex quihoui, Dianthus caryophyllus and Loropetalum chinensis.

  18. Improving the extraction and purification of immunoglobulin G by the use of ionic liquids as adjuvants in aqueous biphasic systems.

    Science.gov (United States)

    Ferreira, Ana M; Faustino, Vânia F M; Mondal, Dibyendu; Coutinho, João A P; Freire, Mara G

    2016-10-20

    Immunoglobulins G (IgG) could become widespread biopharmaceuticals if cost-efficient processes for their extraction and purification are available. In this work, aqueous biphasic systems (ABS) composed of polyethylene glycols and a buffered salt, and with ionic liquids (ILs) as adjuvants, have been studied as alternative extraction and purification platforms of IgG from a rabbit serum source. Eleven ILs were investigated to provide insights on the chemical features which maximize the IgG partitioning. It is shown that in polymer-salt systems pure IgG preferentially partitions to the polymer-rich phase; yet, the complete extraction was never attained. Remarkably, after the addition of 5wt% of adequate ILs to polymer-salt ABS, the complete extraction of pure IgG in a single-step was accomplished. The best systems and conditions were then applied to the extraction and purification of IgG directly from rabbit serum samples. The complete extraction of IgG in a single-step was maintained while its purity in the polymer-rich phase was enhanced by ca. 37% as compared to the IL-free ABS. The antibody stability was also evaluated revealing that appropriate ILs are able to maintain the IgG stability and can be used as phase-forming components of ABS when envisaging the purification of high-cost biopharmaceuticals.

  19. NO.sub.x catalyst and method of suppressing sulfate formation in an exhaust purification system

    Science.gov (United States)

    Balmer-Millar, Mari Lou; Park, Paul W.; Panov, Alexander G.

    2007-06-26

    The activity and durability of a zeolite lean-burn NOx catalyst can be increased by loading metal cations on the outer surface of the zeolite. However, the metal loadings can also oxidize sulfur dioxide to cause sulfate formation in the exhaust. The present invention is a method of suppressing sulfate formation in an exhaust purification system including a NO.sub.x catalyst. The NO.sub.x catalyst includes a zeolite loaded with at least one metal. The metal is selected from among an alkali metal, an alkaline earth metal, a lanthanide metal, a noble metal, and a transition metal. In order to suppress sulfate formation, at least a portion of the loaded metal is complexed with at least one of sulfate, phosphate, and carbonate.

  20. Rapid purification of quantum systems by measuring in a feedback-controlled unbiased basis

    CERN Document Server

    Combes, Joshua; Jacobs, Kurt; O'Connor, Anthony J

    2010-01-01

    Rapid-purification by feedback --- specifically, reducing the mean impurity faster than by measurement alone --- can be achieved by making the eigenbasis of the density matrix to be unbiased relative to the measurement basis. Here we further examine the protocol introduced by Combes and Jacobs [Phys.~Rev.~Lett.~{\\bf 96}, 010504 (2006)] involving continuous measurement of the observable $J_z$ for a $D$-dimensional system. We rigorously re-derive the lower bound $(2/3)(D+1)$ on the achievable speed-up factor, and also an upper bound, namely $D^2/2$, for all feedback protocols that use measurements in unbiased bases. Finally we extend our results to $n$ independent measurements on a register of $n$ qubits, and derive an upper bound on the achievable speed-up factor that scales linearly with $n$.

  1. Study on Purification Diatomite with nitric acid by Thermal Closed System

    Directory of Open Access Journals (Sweden)

    Kuang Meng

    2016-01-01

    Full Text Available In this research, a purification approach using nitric acid leaching at thermal closed system was developed to improve the porous structure of raw diatomite by removal of impurities from its surface and clogged pores. The feasibility and efficiency of this approach were determined by XRF for chemical constitution of diatomite, SEM for morphology and BET for specific surface area of purified diatomite. The investigations indicated that the content of SiO2 was in order of 85.14% for raw diatomite and 98% for purified diatomite, the content of Fe2O3 decreases after purified; the integrity of the porous structure was confirmed by SEM, and increase in specific surface area from 18m2·g-1 to 36m2·g-1.

  2. Single-Step Purification of Ovalbumin from Egg White Using Aqueous Biphasic Systems.

    Science.gov (United States)

    Pereira, Matheus M; Cruz, Rafaela A P; Almeida, Mafalda R; Lima, Álvaro S; Coutinho, João A P; Freire, Mara G

    2016-06-01

    The ability of aqueous biphasic systems (ABS) composed of polyethylene glycols of different molecular weights (PEG 400, 600 and 1000) and buffered aqueous solutions of potassium citrate/citric acid (pH = 5.0 - 8.0) to selectively extract ovalbumin from egg white was here investigated. Phase diagrams, tie-lines and tie-line lengths were determined at 25ºC and the partitioning of ovalbumin in these systems was then evaluated. Aiming at optimizing the selective extraction of ovalbumin in the studied ABS, factors such as pH, PEG molecular weight and amount of the phase-forming components were initially investigated with pure commercial ovalbumin. In almost all ABS, it was observed a preferential partitioning of ovalbumin to the polymer-rich phase, with extraction efficiencies higher than 90%. The best ABS were then applied in the purification of ovalbumin from the real egg white matrix. In order to ascertain on the ovalbumin purity and yield, sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and size exclusion high performance liquid chromatography (SE-HPLC) analyses were conducted, confirming that the isolation/purification of ovalbumin from egg white was completely achieved in a single-step with a recovery yield of 65%. The results obtained show that polymer-salt-based ABS allow the selective extraction of ovalbumin from egg white with a simpler approach and better performance than previously reported. Finally, it is shown that ovalbumin can be completely recovered from the PEG-rich phase by an induced precipitation using an inexpensive and sustainable separation platform which can be easily applied on an industrial scale.

  3. NSLS-II RF Cryogenic System

    Energy Technology Data Exchange (ETDEWEB)

    Rose, J.; Dilgen, T.; Gash, B.; Gosman, J.; Mortazavi, P.; Papu, J.; Ravindranath, V.; Sikora, R.; Sitnikov, A.; Wilhelm, H.; Jia, Y.; Monroe, C.

    2015-05-03

    The National Synchrotron Light Source II is a 3 GeV X-ray user facility commissioned in 2014. A new helium refrigerator system has been installed and commissioned to support the superconducting RF cavities in the storage ring. Special care was taken to provide very stable helium and LN2 pressures and flow rates to minimize microphonics and thermal effects at the cavities. Details of the system design along with commissioning and early operations data will be presented.

  4. Design of functional guanidinium ionic liquid aqueous two-phase systems for the efficient purification of protein

    Energy Technology Data Exchange (ETDEWEB)

    Ding, Xueqin; Wang, Yuzhi, E-mail: wyzss@hnu.edu.cn; Zeng, Qun; Chen, Jing; Huang, Yanhua; Xu, Kaijia

    2014-03-01

    Graphical abstract: - Highlights: • A series of novel cationic functional hexaalkylguanidinium ionic liquids and anionic functional tetraalkylguanidinium ionic liquids have been synthesized. • Functional guanidinium ionic liquid aqueous two-phase systems have been first designed for the purification of protein. • Mechanisms and performances of the process were researched. • Simple, green, safety and presents better purified ability than ordinary process. • A potential efficient platform for protein purification and related studies. - Abstract: A series of novel cationic functional hexaalkylguanidinium ionic liquids and anionic functional tetraalkylguanidinium ionic liquids have been devised and synthesized based on 1,1,3,3-tetramethylguanidine. The structures of the ionic liquids (ILs) were confirmed by {sup 1}H nuclear magnetic resonance ({sup 1}H NMR) and 13C nuclear magnetic resonance (13C NMR) and the production yields were all above 90%. Functional guanidinium ionic liquid aqueous two-phase systems (FGIL-ATPSs) have been first designed with these functional guanidinium ILs and phosphate solution for the purification of protein. After phase separation, proteins had transferred into the IL-rich phase and the concentrations of proteins were determined by measuring the absorbance at 278 nm using an ultra violet visible (UV–vis) spectrophotometer. The advantages of FGIL-ATPSs were compared with ordinary ionic liquid aqueous two-phase systems (IL-ATPSs). The proposed FGIL-ATPS has been applied to purify lysozyme, trypsin, ovalbumin and bovine serum albumin. Single factor experiments were used to research the effects of the process, such as the amount of ionic liquid (IL), the concentration of salt solution, temperature and the amount of protein. The purification efficiency reaches to 97.05%. The secondary structure of protein during the experimental process was observed upon investigation using UV–vis spectrophotometer, Fourier-transform infrared

  5. The Beam Inhibit System for TTF II

    CERN Document Server

    Nölle, D; Neumann, R; Pugachov, D; Wittenburg, K; Wendt, M; Werner, M; Schlarb, H; Staack, M

    2003-01-01

    The new generation of light sources based on SASE Free-Electron-Lasers driven by LINACs operate with electron beams with high beam currents and duty cycles. This is especially true for the superconducting machines like TTF II and the X-RAY FEL, under construction or planning at DESY. Elaborate fast protections systems are required not only to protect the machine from electron beams hitting and destroying the vacuum chamber, but also to prevent the machine from running at high loss levels, dangerous for components like the FEL undulator. This paper will give an overview over the different protection systems currently under construction for TTF II. The very fast systems, based on transmission measurements and distributed loss detection monitors, will be described in detail. This description will include the fast electronics to collect and to transmit the different interlock signals.

  6. Design of functional guanidinium ionic liquid aqueous two-phase systems for the efficient purification of protein.

    Science.gov (United States)

    Ding, Xueqin; Wang, Yuzhi; Zeng, Qun; Chen, Jing; Huang, Yanhua; Xu, Kaijia

    2014-03-07

    A series of novel cationic functional hexaalkylguanidinium ionic liquids and anionic functional tetraalkylguanidinium ionic liquids have been devised and synthesized based on 1,1,3,3-tetramethylguanidine. The structures of the ionic liquids (ILs) were confirmed by (1)H nuclear magnetic resonance ((1)H NMR) and 13C nuclear magnetic resonance (13C NMR) and the production yields were all above 90%. Functional guanidinium ionic liquid aqueous two-phase systems (FGIL-ATPSs) have been first designed with these functional guanidinium ILs and phosphate solution for the purification of protein. After phase separation, proteins had transferred into the IL-rich phase and the concentrations of proteins were determined by measuring the absorbance at 278 nm using an ultra violet visible (UV-vis) spectrophotometer. The advantages of FGIL-ATPSs were compared with ordinary ionic liquid aqueous two-phase systems (IL-ATPSs). The proposed FGIL-ATPS has been applied to purify lysozyme, trypsin, ovalbumin and bovine serum albumin. Single factor experiments were used to research the effects of the process, such as the amount of ionic liquid (IL), the concentration of salt solution, temperature and the amount of protein. The purification efficiency reaches to 97.05%. The secondary structure of protein during the experimental process was observed upon investigation using UV-vis spectrophotometer, Fourier-transform infrared spectroscopy (FT-IR) and circular dichroism spectrum (CD spectrum). The precision, stability and repeatability of the process were investigated. The mechanisms of purification were researched by dynamic light scattering (DLS), determination of the conductivity and transmission electron microscopy (TEM). It was suggested that aggregation and embrace phenomenon play a significant role in the purification of proteins. All the results show that FGIL-ATPSs have huge potential to offer new possibility in the purification of proteins.

  7. Improvement of xenon purification system using a combination of a pulse tube refrigerator and a coaxial heat exchanger

    CERN Document Server

    Chen, Wan-Ting; Cussonneau, J -P; Donnard, J; Duval, S; Lemaire, O; Calloch, M Le; Ray, P Le; Mohamad-Hadi, A -F; Morteau, E; Oger, T; Scotto-Lavina, L; Stutzmann, J -S; Thers, D; Briend, P; Haruyama, T; Mihara, S; Tauchi, T

    2012-01-01

    We have developed a compact cryogenic system with a pulse tube refrigerator and a coaxial heat exchanger. This liquefaction-purification system not only saves the cooling power used to reach high gaseous recirculation rate, but also reduces the impurity level with high speed. The heat exchanger operates with an efficiency of 99%, which indicates the possibility for fast xenon gas recirculation in a highpressurized large-scale xenon storage with much less thermal losses.

  8. The study of recirculating aquaculture system in pond and its purification effect

    Science.gov (United States)

    Qu, Jiangqi; Zhang, Qingjing; Jia, Chengxia; Liu, Pan; Yang, Mu

    2017-05-01

    In this paper, a recirculating aquaculture purification system (RAPS) was designed to solve the problems of aquaculture pollution and shortage of freshwater resource according to the characteristic of northern freshwater ponds of China. The system were arranged in series and composed of high density culture pond, deposit pond, floating and submerged plant pond, ecological floating bed pond and biofilm filtrate pond. At the fish density of 20~30kg/m3 in the high density culture pond, the water quality parameters were monitored seasonally. The results indicated that the removal rate of total nitrogen, total phosphorus, ammonia nitrogen and nitrite nitrogen in the recirculating aquaculture system were 69.59%, 77.89%, 72.54% and 68.68%, respectively. The floating and submerged plant pond and ecological floating bed pond can remove TN and TP obviously, and increase dissolved oxygen and transparency significantly. And the biofilm filtrate pond has good effect of removing ammonium nitrogen and nitrite nitrogen, meanwhile, the microbial communities in the recirculating aquaculture system regulate on the water quality. Therefore, the RAPS show significant effects on water saving and pollution emission reducing.

  9. Expression and Purification of ZNF191(243-368) in Three Expression Systems

    Institute of Scientific and Technical Information of China (English)

    ZHAO Dong-Xin; TENG Xin-Cheng; DING Zhi-Chun; HUANG Zhong-Xian

    2007-01-01

    ZNF191(243-368), a new human zinc finger protein, probably relates to some hereditary diseases and cancers.To obtain adequate amount of ZNF 191 (243-368) for the study of its property, structure and function, three different expression systems of inclusion-body, glutathione S-transferase (GST), and hexahistidine (6 × His) were used and compared. Among these systems, the expression level of ZNF191(243-368) was increased in inclusion body system under a higher isopropylthio-β-D-galactoside (IPTG) concentration, but the non-target proteins were also increased more, which made its purification more difficult and the yield lower. The expression of His-tag fusion protein was almost not affected by IPTG concentration, temperature and inducing time. At a high IPTG concentration the highest expression yield for GST fusion protein was obtained. And the fusion proteins can be partially purified by a single affinity chromatography step. The fusion protein systems show advantages for expression of these proteins.

  10. Evaluation of systems for purification of fuel gas from anaerobic digestion. Engineering report

    Energy Technology Data Exchange (ETDEWEB)

    Ashare, E.; Augenstein, D. C.; Yeung, J. C.; Hossan, R. J.; Duret, G. L.

    1978-07-30

    Fuel gas obtained from the anaerobic digestion of waste materials usually needs to be treated before being transmitted in existing pipeline systems. The purification scheme involves the removal of carbon dioxide, hydrogen sulfide, and moisture from the digester gas to meet pipeline specifications. Gas treatment systems for the handling of 0.1, 1.0, and 3.0 MM Scfd of raw feed gas and product delivery pressures of 125 and 1000 psia were considered in this study. From the results of an economic and technical analysis of these systems, physical absorption systems, particularly water scrubbing, were found to be the most economical system for the treatment of digester gas with the flow streams considered. The Fluor Solvent process was economically comparable with the water scrubbing process for high pressure applications, but the value presented had a large uncertainty. The commercial chemical absorption systems were less economical due to high heat requirements for solvent regeneration. Among the chemical absorption processes, the hot potassium systems were found to be more economical than the amine system. The molecular sieve adsorption process was economically similar to the chemical absorption process. The GE membrane separation process was economically comparable for both high and low pressure apllications, but this system has not been evaluated in real-life conditions. Since no kinetic and equilibrium data were available for the phosphate buffer system, estimates had to be assumed. Using what were believed to be very conservative estimates, this chemical absorption process was found to be economically competitive with the water scrubbing process for treating gas with a delivery pressure of 125 psia.The cost analyses in this study were based on the assumption of no recovery of the CO/sub 2/ by-product. It was found that credits for the recovery of the CO/sub 2/ could be significant.

  11. PEP-II RF feedback system simulation

    Energy Technology Data Exchange (ETDEWEB)

    Tighe, R. [Stanford Linear Accelerator Center, Menlo Park, CA (United States)

    1996-08-01

    A model containing the fundamental impedance of the PEP-II cavity along with the longitudinal beam dynamics and RF feedback system components is in use. It is prepared in a format allowing time-domain as well as frequency-domain analysis and full graphics capability. Matlab and Simulink are control system design and analysis programs (widely available) with many built-in tools. The model allows the use of compiled C-code modules for compute intensive portions. We desire to represent as nearly as possible the components of the feedback system including all delays, sample rates and applicable nonlinearities. (author)

  12. Purification of chlorogenic acid in Flos Lonicerae with system of polar ordered resins

    Institute of Scientific and Technical Information of China (English)

    XIANG Zhi-nan; ZHAN Yu; NING Zheng-xiang

    2007-01-01

    A system of polar ordered resins was established for purification of chlorogenic acid in Flos Lonicerae. It was composed of three reversed phase resins, AB-8, DM-130 and NKA-9, representative for their gradually increased polarity and selectivity. A method of RP-HPLC was used for determination of chlorogenic acid. And the performance of adsorption and desorption for chlorogenic acid with the system of polar ordered resins was studied. Furthermore, the effects of concentration, pH and flow rate of the adsorbate on adsorption ability were researched. It is indicated that the optimum parameters for chlorogenic acid are as follows:pH 3.5 with a flow rate of 2.5 BV/h, the concentration of extract solution at 0.50, 0.40, 0.30 g/L respectively for the adsorptive operation twice, and 6.93, 8.66, 10.39 mol/L ethanol used as gradient eluants. The purity of resulted product of chlorogenic acid arrives 70.20% with yield of 89.79%. With simple procedures, low costs and high purity product, the method of system of polar ordered resins followed by sequential reversed phase separations can be used to refine the chlorogenic acid in the extraction of Flos Lonicerae.

  13. Purification and assays of Rhodobacter capsulatus RegB-RegA two-component signal transduction system.

    Science.gov (United States)

    Swem, Lee R; Swem, Danielle L; Wu, Jiang; Bauer, Carl E

    2007-01-01

    Two-component signal-transduction systems, composed of a histidine-sensor kinase and a DNA-binding response regulator, allow bacteria to detect environmental changes and adjust cellular physiology to live more efficiently in a broad distribution of niches. Although many two-component signal-transduction systems are known, a limited number of signals that stimulate these systems have been discovered. This chapter describes the purification and characterization of the predominant two-component signal-transduction system utilized by Rhodobacter capsulatus, a nonsulfur purple photosynthetic bacterium. Specifically, we explain the overexpression, detergent solubilization, and purification of the full-length membrane-spanning histidine-sensor kinase RegB. We also provide a method to measure autophosphorylation of RegB and discern the effect of its signal molecule, ubiquinone, on autophosphorylation levels. In addition we describe the overexpression and purification of the cognate response regulator RegA and a technique used to visualize the phosphotransfer reaction from RegB to RegA.

  14. Simulation of a hydrogen production and purification system for a PEM fuel-cell using bioethanol as raw material

    Energy Technology Data Exchange (ETDEWEB)

    Giunta, Pablo; Amadeo, Norma; Laborde, Miguel [Facultad de Ingenieria, Universidad de Buenos Aires, Laboratorio de Procesos Cataliticos, Pabellon de Industrias, Ciudad Universitaria, 1428 Buenos Aires (Argentina); Mosquera, Carlos [Facultad de Ingenieria, Universidad de Buenos Aires, Departamento de Fisica, 1063 Buenos Aires (Argentina)

    2007-01-10

    A process to produce 'fuel-cell grade' hydrogen from ethanol steam reforming is analyzed from a thermodynamic point of view. The hydrogen purification process consists of WGS and COPROX reactors. Equations to evaluate the efficiency of the system, including the fuel cell, are presented. A heat exchange network is proposed in order to improve the exploitation of the available power. The effect of key variables such as the reformer temperature and the ethanol/water molar feed ratio on the fuel-cell efficiency is discussed. Results show that it is feasible to carry out the energy integration of the hydrogen catalytic production and purification-PEM fuel-cell system, using ethanol as raw material. The technology of 'fuel-cell grade' hydrogen production using ethanol as raw material is a very attractive alternative to those technologies based in fossil fuels. (author)

  15. Papain digestion of crude Trichoderma reesei cellulase: Purification and properties of cellobiohydrolase I and II core proteins

    Energy Technology Data Exchange (ETDEWEB)

    Woodward, J.; Brown, J.P.; Evans, B.R.; Affholter, K.A.

    1992-01-01

    Papain digestion of a crude Trichoderma reesei cellulose preparation followed by gel filtration on a Superdex column resulted in the separation of cellobiohydrolase (CBH) I and II core proteins (cp). They were further purified to apparent homogeneity by chromatofocusing. N-terminal protein sequencing of the CBH II cp preparation confirmed its identity. A comparison of the catalytic activity and cellulose-binding ability of these core proteins was made. The major differences between them were the findings that CBH II cp possessed a sixfold higher specific activity toward p-nitrophenylcellobioside than the native CBH II preparation and still bound to microcrystalline cellulose, unlike CBH I cp. Neither CBH I cp nor CBH II cp had activity toward carboxymethylcellulose, but both were able to hydrolyze barley b-glucan. These data suggest that removal of the cellulose-binding domain and hinge region from CBH I and II have different effects on their properties.

  16. Papain digestion of crude Trichoderma reesei cellulase: Purification and properties of cellobiohydrolase I and II core proteins

    Energy Technology Data Exchange (ETDEWEB)

    Woodward, J.; Brown, J.P.; Evans, B.R.; Affholter, K.A.

    1992-12-01

    Papain digestion of a crude Trichoderma reesei cellulose preparation followed by gel filtration on a Superdex column resulted in the separation of cellobiohydrolase (CBH) I and II core proteins (cp). They were further purified to apparent homogeneity by chromatofocusing. N-terminal protein sequencing of the CBH II cp preparation confirmed its identity. A comparison of the catalytic activity and cellulose-binding ability of these core proteins was made. The major differences between them were the findings that CBH II cp possessed a sixfold higher specific activity toward p-nitrophenylcellobioside than the native CBH II preparation and still bound to microcrystalline cellulose, unlike CBH I cp. Neither CBH I cp nor CBH II cp had activity toward carboxymethylcellulose, but both were able to hydrolyze barley b-glucan. These data suggest that removal of the cellulose-binding domain and hinge region from CBH I and II have different effects on their properties.

  17. The CDMS II data acquisition system

    Energy Technology Data Exchange (ETDEWEB)

    Bauer, D.A.; /Fermilab; Burke, S.; /UC, Santa Barbara; Cooley, J.; /Southern Methodist U.; Crisler, M.; /Fermilab; Cushman, P.; /Minnesota U.; DeJongh, F.; /Fermilab; Duong, L.; /Minnesota U.; Ferril, R.; /UC, Santa Barbara; Golwala, S.R.; /Caltech; Hall, J.; /Fermilab; Holmgren, D.; /Fermilab /Texas A-M

    2011-01-01

    The Data Acquisition System for the CDMS II dark matter experiment was designed and built when the experiment moved to its new underground installation at the Soudan Lab. The combination of remote operation and increased data load necessitated a completely new design. Elements of the original LabView system remained as stand-alone diagnostic programs, but the main data processing moved to a VME-based system with custom electronics for signal conditioning, trigger formation and buffering. The data rate was increased 100-fold and the automated cryogenic system was linked to the data acquisition. A modular server framework with associated user interfaces was implemented in Java to allow control and monitoring of the entire experiment remotely.

  18. Type II Collagen and Gelatin from Silvertip Shark (Carcharhinus albimarginatus) Cartilage: Isolation, Purification, Physicochemical and Antioxidant Properties

    OpenAIRE

    Elango Jeevithan; Bin Bao; Yongshi Bu; Yu Zhou; Qingbo Zhao; Wenhui Wu

    2014-01-01

    Type II acid soluble collagen (CIIA), pepsin soluble collagen (CIIP) and type II gelatin (GII) were isolated from silvertip shark (Carcharhinus albimarginatus) cartilage and examined for their physicochemical and antioxidant properties. GII had a higher hydroxyproline content (173 mg/g) than the collagens and cartilage. CIIA, CIIP and GII were composed of two identical α1 and β chains and were characterized as type II. Amino acid analysis of CIIA, CIIP and GII indicated imino acid contents of...

  19. Chloroplast ribonucleoproteins (RNPs) as phosphate acceptors for casein kinase II: purification by ssDNA-cellulose column chromatography.

    Science.gov (United States)

    Kanekatsu, M; Ezumi, A; Nakamura, T; Ohtsuki, K

    1995-12-01

    Using ssDNA-cellulose column chromatography, a 34 kDa ribonucleoprotein (p34) has been purified from a 0.4 M KCl crude extract of spinach chloroplasts as an effective phosphate acceptor for casein kinase II (CK-II) in vitro. Monomeric and oligomeric CK-IIs were copurified with p34 by the column chromatography and the kinases were separated from p34 by means of Mono Q column chromatography. It was found that (i) the purified p34 (pI 4.9) was phosphorylated specifically by CK-II in vitro; and (ii) similar polypeptides, such as p35 (pI 4.7) and p39 (pI 4.9) in maize and p33 (pI 4.7) in liverwort, were detected as ssDNA-binding chloroplast proteins phosphorylated by CK-II in vitro. The findings suggest that (i) RNPs that function as phosphate acceptors for CK-II exist commonly in chloroplasts among plant cells; and (ii) the physiological activity of RNPs is regulated by their specific phosphorylation by CK-II in chloroplasts.

  20. Fractionated plasma separation and adsorption system: a novel system for blood purification to remove albumin bound substances.

    Science.gov (United States)

    Falkenhagen, D; Strobl, W; Vogt, G; Schrefl, A; Linsberger, I; Gerner, F J; Schoenhofen, M

    1999-01-01

    The removal of albumin bound substances has gained increasing interest in different diseases, especially in acute and chronic liver disease. Therefore, a new system, the fractionated plasma separation and adsorption (FPSA) system, was developed based on combined membrane and adsorbent blood purification techniques. The most important contribution to the FPSA system was the development of a new polysulfone hollow-fiber filter, which is characterized by a sieving coefficient of 0.89 for human serum albumin (HSA) but only of 0.17 for fibrinogen, and 0 (zero) for IgM immunoglobulins. Using a closed filtrate circuit connected to the new polysulfone filter which integrates 1 or 2 adsorption columns and also a high flux dialyzer adapted to a dialysis machine, the FPSA system opens excellent possibilities for the relatively specific removal of albumin bound substances from the blood such as albumin bound bilirubin or even tryptophan. In comparison to other systems (for example, the Molecular Adsorbent Recirculating System [MARS] and albumin dialysis systems), the FPSA system enables much higher elimination of strongly bound albumin substances. The first clinical investigations have recently started based on a modified dialysis machine designed with all necessary safety measures.

  1. AM-DMC-AMPS Multi-Functionalized Magnetic Nanoparticles for Efficient Purification of Complex Multiphase Water System

    Science.gov (United States)

    Ge, Yuru; Li, Yushu; Zu, Baiyi; Zhou, Chaoyu; Dou, Xincun

    2016-04-01

    Complex multiphase waste system purification, as one of the major challenges in many industrial fields, urgently needs an efficient one-step purification method to remove several pollutants simultaneously and efficiently. Multi-functionalized magnetic nanoparticles, Fe3O4@SiO2-MPS-AM-DMC-AMPS, were facilely prepared via a one-pot in situ polymerization of three different functional monomers, AM, DMC, and AMPS, on a Fe3O4@SiO2-MPS core-shell structure. The multi-functionalized magnetic nanoparticles (MNPs) are proven to be a highly effective purification agent for oilfield wastewater, an ideal example of industrial complex multiphase waste system containing cations, anions, and organic pollutants. Excellent overall removal efficiencies for both cations, including K+, Ca2+, Na+, and Mg2+ of 80.68 %, and anions, namely Cl- and SO4 2-, of 85.18 % along with oil of 97.4 % were shown. The high removal efficiencies are attributed to the effective binding of the functional groups from the selected monomers with cations, anions, and oil emulsions.

  2. AM-DMC-AMPS Multi-Functionalized Magnetic Nanoparticles for Efficient Purification of Complex Multiphase Water System.

    Science.gov (United States)

    Ge, Yuru; Li, Yushu; Zu, Baiyi; Zhou, Chaoyu; Dou, Xincun

    2016-12-01

    Complex multiphase waste system purification, as one of the major challenges in many industrial fields, urgently needs an efficient one-step purification method to remove several pollutants simultaneously and efficiently. Multi-functionalized magnetic nanoparticles, Fe3O4@SiO2-MPS-AM-DMC-AMPS, were facilely prepared via a one-pot in situ polymerization of three different functional monomers, AM, DMC, and AMPS, on a Fe3O4@SiO2-MPS core-shell structure. The multi-functionalized magnetic nanoparticles (MNPs) are proven to be a highly effective purification agent for oilfield wastewater, an ideal example of industrial complex multiphase waste system containing cations, anions, and organic pollutants. Excellent overall removal efficiencies for both cations, including K(+), Ca(2+), Na(+), and Mg(2+) of 80.68 %, and anions, namely Cl(-) and SO4 (2-), of 85.18 % along with oil of 97.4 % were shown. The high removal efficiencies are attributed to the effective binding of the functional groups from the selected monomers with cations, anions, and oil emulsions.

  3. Extraction and purification of wheat-esterase using aqueous two-phase systems of ionic liquid and salt.

    Science.gov (United States)

    Jiang, Bin; Feng, Zhibiao; Liu, Chunhong; Xu, Yingcao; Li, Dongmei; Ji, Guo

    2015-05-01

    To explore a new and simple rapid extraction and purification technique for wheat-esterase, an ionic liquids (ILs)-based aqueous two-phase system (ATPS) was developed for the purification of wheat-esterase from wheat extracts. Effects of various process parameters such as the concentrations of [Bmim]BF4, the types and concentrations of phase-forming salt, the system pH and the temperature on partitioning of wheat-esterase were evaluated. The obtained data indicated that wheat-esterase was preferentially partitioned into the ILs-rich phase and the ATPS composed of 20 % [Bmim]BF4 (w/w) and 25 % (w/w) NaH2PO4(pH = 4.8) showed good selectivity on wheat-esterase. Under the optimum conditions, wheat-esterase was purified with an acceptable yield (88.93 %), but produced wheat-esterase was 4.23 times as pure. It was obvious that temperature shows little influence on the purification between 10 and 50 °C. Sephadex G-150FF revealed that the band intensity of contaminating proteins in ATPS fraction almost disappeared. Therefore, ILs-based ATPS was an effective method for partitioning and recovery of wheat-esterase from wheat crude extracts.

  4. DOE Hydrogen, Fuel Cells and Infrastructure Technologies Program Integrated Hydrogen Production, Purification and Compression System

    Energy Technology Data Exchange (ETDEWEB)

    Tamhankar, Satish; Gulamhusein, Ali; Boyd, Tony; DaCosta, David; Golben, Mark

    2011-06-30

    The project was started in April 2005 with the objective to meet the DOE target of delivered hydrogen of <$1.50/gge, which was later revised by DOE to $2-$3/gge range for hydrogen to be competitive with gasoline as a fuel for vehicles. For small, on-site hydrogen plants being evaluated at the time for refueling stations (the 'forecourt'), it was determined that capital cost is the main contributor to the high cost of delivered hydrogen. The concept of this project was to reduce the cost by combining unit operations for the entire generation, purification, and compression system (refer to Figure 1). To accomplish this, the Fluid Bed Membrane Reactor (FBMR) developed by MRT was used. The FBMR has hydrogen selective, palladium-alloy membrane modules immersed in the reformer vessel, thereby directly producing high purity hydrogen in a single step. The continuous removal of pure hydrogen from the reformer pushes the equilibrium 'forward', thereby maximizing the productivity with an associated reduction in the cost of product hydrogen. Additional gains were envisaged by the integration of the novel Metal Hydride Hydrogen Compressor (MHC) developed by Ergenics, which compresses hydrogen from 0.5 bar (7 psia) to 350 bar (5,076 psia) or higher in a single unit using thermal energy. Excess energy from the reformer provides up to 25% of the power used for driving the hydride compressor so that system integration improved efficiency. Hydrogen from the membrane reformer is of very high, fuel cell vehicle (FCV) quality (purity over 99.99%), eliminating the need for a separate purification step. The hydride compressor maintains hydrogen purity because it does not have dynamic seals or lubricating oil. The project team set out to integrate the membrane reformer developed by MRT and the hydride compression system developed by Ergenics in a single package. This was expected to result in lower cost and higher efficiency compared to conventional hydrogen production

  5. A generic system for the expression and purification of soluble and stable influenza neuraminidase.

    Directory of Open Access Journals (Sweden)

    Peter M Schmidt

    Full Text Available The influenza surface glycoprotein neuraminidase (NA is essential for the efficient spread of the virus. Antiviral drugs such as Tamiflu (oseltamivir and Relenza (zanamivir that inhibit NA enzyme activity have been shown to be effective in the treatment of influenza infections. The recent 'swine flu' pandemic and world-wide emergence of Tamiflu-resistant seasonal human influenza A(H1N1 H(274Y have highlighted the need for the ongoing development of new anti-virals, efficient production of vaccine proteins and novel diagnostic tools. Each of these goals could benefit from the production of large quantities of highly pure and stable NA. This publication describes a generic expression system for NAs in a baculovirus Expression Vector System (BEVS that is capable of expressing milligram amounts of recombinant NA. To construct NAs with increased stability, the natural influenza NA stalk was replaced by two different artificial tetramerization domains that drive the formation of catalytically active NA homotetramers: GCN4-pLI from yeast or the Tetrabrachion tetramerization domain from Staphylothermus marinus. Both recombinant NAs are secreted as FLAG-tagged proteins to allow for rapid and simple purification. The Tetrabrachion-based NA showed good solubility, increased stability and biochemical properties closer to the original viral NA than the GCN4-pLI based construct. The expressed quantities and high quality of the purified recombinant NA suggest that this expression system is capable of producing recombinant NA for a broad range of applications including high-throughput drug screening, protein crystallisation, or vaccine development.

  6. Purification Performance and Production of a Re-circulating Pond Aquaculture System Based on Paddy Field

    Directory of Open Access Journals (Sweden)

    Gu Li

    2012-10-01

    Full Text Available Developing improved aquaculture systems with a more efficient use of water and less environmental impact is becoming a crying need. A re-circulating aquaculture system consisting of paddy field and fish pond is a new culture mode due to aquaculture combing with agriculture. The present study focused on the purification capacity of the paddy field on nitrogen, phosphorus and organic matter, the fluctuation trend of water quality conditions during the whole rearing process and the culture efficacy of the main culture species of grass carp (Ctenopharyngodon idella. The results were as follows: under a flow rate of 1.4-5.5 m3/h for the recirculation treatment, the average removal rate of ammonia nitrogen, nitrate nitrogen, total nitrogen, total phosphorus and biochemical oxygen demand for the aquaculture effluent amounted to 40.5, 43.5, 31.9, 23.9, 20.7 and 52.4%, respectively, But the dissolved oxygen content in the rice fields increased obviously. During the whole process of fish rearing, the main physicochemical parameters of water quality for the experimental ponds were all maintained at a suitable level for the growth of the grass carp. In addition, there were significant differences (p<0.05 in DO, TSS, NH4+ -N, NO--N, BOD5 and Chl-&alpha between the experimental and control ponds. As far as the yield per unit and survival rate was concerned, the level of the experimental ponds was obviously higher than that of the control, while the feed conversion ratio displayed the opposite trend. Overall, the new aquaculture system realized the double aims of water reuse and the reduction of waste water discharge.

  7. The CDF-II silicon tracking system

    CERN Document Server

    Nelson, T K

    2002-01-01

    The CDF silicon tracking system for Run II of the Fermilab Tevatron consists of eight layers arranged in cylinders spanning radii from 1.35 to 28 cm, and lengths from 90 cm to nearly 2 m for a total of 6 m sup 2 of silicon and 722,000 readout channels. With an innermost layer (Layer 00) utilizing radiation tolerant p sup + -in-n silicon and low-mass readout cables between the sensors and readout electronics, double-sided vertexing layers (SVXII) designed for use with a deadtimeless secondary-vertex trigger, and outermost layers (Intermediate Silicon Layers) utilizing mass-producible modules attached to a carbon fiber spaceframe, this system is a starting point for the next generation of silicon trackers for the LHC and Tevatron.

  8. Bacterial-based systems for expression and purification of recombinant Lassa virus proteins of immunological relevance

    Directory of Open Access Journals (Sweden)

    Cashman Kathleen A

    2008-06-01

    Full Text Available Abstract Background There is a significant requirement for the development and acquisition of reagents that will facilitate effective diagnosis, treatment, and prevention of Lassa fever. In this regard, recombinant Lassa virus (LASV proteins may serve as valuable tools in diverse antiviral applications. Bacterial-based systems were engineered for expression and purification of recombinant LASV nucleoprotein (NP, glycoprotein 1 (GP1, and glycoprotein 2 (GP2. Results Full-length NP and the ectodomains of GP1 and GP2 were generated as maltose-binding protein (MBP fusions in the Rosetta strains of Escherichia coli (E. coli using pMAL-c2x vectors. Average fusion protein yields per liter of culture for MBP-NP, MBP-GP1, and MBP-GP2 were 10 mg, 9 mg, and 9 mg, respectively. Each protein was captured from cell lysates using amylose resin, cleaved with Factor Xa, and purified using size-exclusion chromatography (SEC. Fermentation cultures resulted in average yields per liter of 1.6 mg, 1.5 mg, and 0.7 mg of purified NP, GP1 and GP2, respectively. LASV-specific antibodies in human convalescent sera specifically detected each of the purified recombinant LASV proteins, highlighting their utility in diagnostic applications. In addition, mouse hyperimmune ascitic fluids (MHAF against a panel of Old and New World arenaviruses demonstrated selective cross reactivity with LASV proteins in Western blot and enzyme-linked immunosorbent assay (ELISA. Conclusion These results demonstrate the potential for developing broadly reactive immunological assays that employ all three arenaviral proteins individually and in combination.

  9. Purification of Water by Aquatic Plants

    OpenAIRE

    Morimitsu, Katsuhito; Kawahigashi, Tatsuo

    2013-01-01

    [Abstract] Water quality purification of many water systems including those occurring in rivers depends to a great degree on water quality purification activities of aquatic plants and microbes. This paper presents a discussion of results, based on laboratory experiments, of purification by aquatic plants.

  10. Large scale expression and purification of mouse melanopsin-L in the baculovirus expression system

    NARCIS (Netherlands)

    Shirzad-Wasei, N.; Oostrum, J. van; Bovee-Geurts, P.H.M.; Wasserman, M.; Bosman, G.J.C.G.M.; Degrip, W.J.

    2013-01-01

    Melanopsin is the mammalian photopigment that primarily mediates non-visual photoregulated physiology. So far, this photopigment is poorly characterized with respect to structure and function. Here, we report large-scale production and purification of the intact long isoform of mouse melanopsin (mel

  11. BEAM CONTAINMENT SYSTEM FOR NSLS-II

    Energy Technology Data Exchange (ETDEWEB)

    Kramer, S.L.; Casey, W.; Job, P.K.

    2010-05-23

    The shielding design for the NSLS-II will provide adequate protection for the full injected beam loss in two periods of the ring around the injection point, but the remainder of the ring is shielded for lower losses of {le} 10% full beam. This will require a system to insure that beam losses don't exceed these levels for a period of time that could cause excessive radiation levels outside the shield walls. This beam containment system will measure, provide a level of control and alarm indication of the beam power losses along the beam path from the source (e-gun, linac) thru the injection system and the storage ring. This system will consist of collimators that will provide limits to (and potentially to measure) the beam miss-steering and control the loss points of the charge and monitors that will measure the average beam current losses along the beam path and alarm when this beam power loss exceeds the level set by the shielding specifications. This will require some new ideas in beam loss detection capability and collimation. The initial planning and R&D program will be presented.

  12. The LCLS-II LLRF System

    Energy Technology Data Exchange (ETDEWEB)

    DooLittle, Lawrence [Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); Huang, G. [Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); Ratti, A. [Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); Serrano, C. [Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); Bachimanchi, Ramakrishna [Thomas Jefferson National Accelerator Facility (TJNAF), Newport News, VA (United States); Hovater, J. Curt [Thomas Jefferson National Accelerator Facility (TJNAF), Newport News, VA (United States); Babel, S. [SLAC National Accelerator Lab., Menlo Park, CA (United States); Hong, B. [SLAC National Accelerator Lab., Menlo Park, CA (United States); Van Winkle, D. [SLAC National Accelerator Lab., Menlo Park, CA (United States); Chase, B. [Fermi National Accelerator Lab. (FNAL), Batavia, IL (United States); Cullerton, E. [FNAL, Batavia, IL; Varghese, P. [FNAL, Batavia, IL

    2015-09-01

    The SLAC National Accelerator Laboratory is planning an upgrade (LCLS-II) to the Linear Coherent Light Source with a 4 GeV CW superconducting (SCRF) linac. The SCRF linac consists of 35 ILC style cryomodules (eight cavities each) for a total of 280 cavities. Expected cavity gradients are 16 MV/m with a loaded QL of ~ 4x107. The RF system will have 3.8 kW solid state amplifiers driving single cavities. To ensure optimum field stability a single-source single-cavity control system has been chosen. It consists of a precision four-channel cavity receiver and RF stations (Forward, Reflected and Drive signals). In order to regulate the resonant frequency variations of the cavities due to He pressure, the tuning of each cavity is controlled by a Piezo actuator and a slow stepper motor. In addition the system (LLRF-amplifier-cavity) is being modeled and cavity microphonic testing has started. This paper describes the LLRF system under consideration, including recent modeling and cavity tests.

  13. Type II collagen and gelatin from silvertip shark (Carcharhinus albimarginatus) cartilage: isolation, purification, physicochemical and antioxidant properties.

    Science.gov (United States)

    Jeevithan, Elango; Bao, Bin; Bu, Yongshi; Zhou, Yu; Zhao, Qingbo; Wu, Wenhui

    2014-06-27

    Type II acid soluble collagen (CIIA), pepsin soluble collagen (CIIP) and type II gelatin (GII) were isolated from silvertip shark (Carcharhinus albimarginatus) cartilage and examined for their physicochemical and antioxidant properties. GII had a higher hydroxyproline content (173 mg/g) than the collagens and cartilage. CIIA, CIIP and GII were composed of two identical α1 and β chains and were characterized as type II. Amino acid analysis of CIIA, CIIP and GII indicated imino acid contents of 150, 156 and 153 amino acid residues per 1000 residues, respectively. Differing Fourier transform infrared (FTIR) spectra of CIIA, CIIP and GII were observed, which suggested that the isolation process affected the secondary structure and molecular order of collagen, particularly the triple-helical structure. The denaturation temperature of GII (32.5 °C) was higher than that of CIIA and CIIP. The antioxidant activity against 1,1-diphenyl-2-picrylhydrazyl radicals and the reducing power of CIIP was greater than that of CIIA and GII. SEM microstructure of the collagens depicted a porous, fibrillary and multi-layered structure. Accordingly, the physicochemical and antioxidant properties of type II collagens (CIIA, CIIP) and GII isolated from shark cartilage were found to be suitable for biomedical applications.

  14. Type II Collagen and Gelatin from Silvertip Shark (Carcharhinus albimarginatus Cartilage: Isolation, Purification, Physicochemical and Antioxidant Properties

    Directory of Open Access Journals (Sweden)

    Elango Jeevithan

    2014-06-01

    Full Text Available Type II acid soluble collagen (CIIA, pepsin soluble collagen (CIIP and type II gelatin (GII were isolated from silvertip shark (Carcharhinus albimarginatus cartilage and examined for their physicochemical and antioxidant properties. GII had a higher hydroxyproline content (173 mg/g than the collagens and cartilage. CIIA, CIIP and GII were composed of two identical α1 and β chains and were characterized as type II. Amino acid analysis of CIIA, CIIP and GII indicated imino acid contents of 150, 156 and 153 amino acid residues per 1000 residues, respectively. Differing Fourier transform infrared (FTIR spectra of CIIA, CIIP and GII were observed, which suggested that the isolation process affected the secondary structure and molecular order of collagen, particularly the triple-helical structure. The denaturation temperature of GII (32.5 °C was higher than that of CIIA and CIIP. The antioxidant activity against 1,1-diphenyl-2-picrylhydrazyl radicals and the reducing power of CIIP was greater than that of CIIA and GII. SEM microstructure of the collagens depicted a porous, fibrillary and multi-layered structure. Accordingly, the physicochemical and antioxidant properties of type II collagens (CIIA, CIIP and GII isolated from shark cartilage were found to be suitable for biomedical applications.

  15. FastTrack to supercritical fluid chromatographic purification: Implementation of a walk-up analytical supercritical fluid chromatography/mass spectrometry screening system in the medicinal chemistry laboratory.

    Science.gov (United States)

    Aurigemma, Christine; Farrell, William

    2010-09-24

    Medicinal chemists often depend on analytical instrumentation for reaction monitoring and product confirmation at all stages of pharmaceutical discovery and development. To obtain pure compounds for biological assays, the removal of side products and final compounds through purification is often necessary. Prior to purification, chemists often utilize open-access analytical LC/MS instruments because mass confirmation is fast and reliable, and the chromatographic separation of most sample constituents is sufficient. Supercritical fluid chromatography (SFC) is often used as an orthogonal technique to HPLC or when isolation of the free base of a compound is desired. In laboratories where SFC is the predominant technique for analysis and purification of compounds, a reasonable approach for quickly determining suitable purification conditions is to screen the sample against different columns. This can be a bottleneck to the purification process. To commission SFC for open-access use, a walk-up analytical SFC/MS screening system was implemented in the medicinal chemistry laboratory. Each sample is automatically screened through six column/method conditions, and on-demand data processing occurs for the chromatographers after each screening method is complete. This paper highlights the "FastTrack" approach to expediting samples through purification.

  16. Purification of two putative type II NADH dehydrogenases with different substrate specificities from alkaliphilic Bacillus pseudofirmus OF4.

    Science.gov (United States)

    Liu, Jun; Krulwich, Terry A; Hicks, David B

    2008-05-01

    A putative Type II NADH dehydrogenase from Halobacillus dabanensis was recently reported to have Na+/H+ antiport activity (and called Nap), raising the possibility of direct coupling of respiration to antiport-dependent pH homeostasis. This study characterized a homologous type II NADH dehydrogenase of genetically tractable alkaliphilic Bacillus pseudofirmus OF4, in which evidence supports antiport-based pH homeostasis that is mediated entirely by secondary antiport. Two candidate type II NADH dehydrogenase genes with canonical GXGXXG motifs were identified in a draft genome sequence of B. pseudofirmus OF4. The gene product designated NDH-2A exhibited homology to enzymes from Bacillus subtilis and Escherichia coli whereas NDH-2B exhibited homology to the H. dabanensis Nap protein and its alkaliphilic Bacillus halodurans C-125 homologue. The ndh-2A, but not the ndh-2B, gene complemented the growth defect of an NADH dehydrogenase-deficient E. coli mutant. Neither gene conferred Na+-resistance on an antiporter-deficient E. coli strain, nor did they confer Na+/H+ antiport activity in vesicle assays. The purified hexa-histidine-tagged gene products were approximately 50 kDa, contained noncovalently bound FAD and oxidized NADH. They were predominantly cytoplasmic in E. coli, consonant with the absence of antiport activity. The catalytic properties of NDH-2A were more consistent with a major respiratory role than those of NDH-2B.

  17. The Belle II SVD data readout system

    Science.gov (United States)

    Thalmeier, R.; Adamczyk, K.; Aihara, H.; Angelini, C.; Aziz, T.; Babu, V.; Bacher, S.; Bahinipati, S.; Barberio, E.; Baroncelli, Ti.; Baroncelli, To.; Basith, A. K.; Batignani, G.; Bauer, A.; Behera, P. K.; Bergauer, T.; Bettarini, S.; Bhuyan, B.; Bilka, T.; Bosi, F.; Bosisio, L.; Bozek, A.; Buchsteiner, F.; Bulla, L.; Casarosa, G.; Ceccanti, M.; Cervenkov, D.; Chendvankar, S. R.; Dash, N.; Divekar, S. T.; Doleźal, Z.; Dutta, D.; Forti, F.; Friedl, M.; Hara, K.; Higuchi, T.; Horiguchi, T.; Irmler, C.; Ishikawa, A.; Jeon, H. B.; Joo, C.; Kandra, J.; Kang, K. H.; Kato, E.; Kawasaki, T.; Kodyś, P.; Kohriki, T.; Koike, S.; Kolwalkar, M. M.; Kvasnićka, P.; Lanceri, L.; Lettenbicher, J.; Lueck, T.; Maki, M.; Mammini, P.; Mayekar, S. N.; Mohanty, G. B.; Mohanty, S.; Morii, T.; Nakamura, K. R.; Natkaniec, Z.; Negishi, K.; Nisar, N. K.; Onuki, Y.; Ostrowicz, W.; Paladino, A.; Paoloni, E.; Park, H.; Pilo, F.; Profeti, A.; Rao, K. K.; Rashevskaya, I.; Rizzo, G.; Rozanska, M.; Sasaki, J.; Sato, N.; Schultschik, S.; Schwanda, C.; Seino, Y.; Shimizu, N.; Stypula, J.; Suzuki, J.; Tanaka, S.; Tanida, K.; Taylor, G. N.; Thomas, R.; Tsuboyama, T.; Uozumi, S.; Urquijo, P.; Vitale, L.; Watanuki, S.; Watson, I. J.; Webb, J.; Wiechczynski, J.; Williams, S.; Würkner, B.; Yamamoto, H.; Yin, H.; Yoshinobu, T.

    2017-02-01

    The Belle II Experiment at the High Energy Accelerator Research Organization (KEK) in Tsukuba, Japan, will explore the asymmetry between matter and antimatter and search for new physics beyond the standard model. 172 double-sided silicon strip detectors are arranged cylindrically in four layers around the collision point to be part of a system which measures the tracks of the collision products of electrons and positrons. A total of 1748 radiation-hard APV25 chips read out 128 silicon strips each and send the analog signals by time-division multiplexing out of the radiation zone to 48 Flash Analog Digital Converter Modules (FADC). Each of them applies processing to the data; for example, it uses a digital finite impulse response filter to compensate line signal distortions, and it extracts the peak timing and amplitude from a set of several data points for each hit, using a neural network. We present an overview of the SVD data readout system, along with front-end electronics, cabling, power supplies and data processing.

  18. Assessment of Integrated Information System (IIS) in organization ...

    African Journals Online (AJOL)

    Assessment of Integrated Information System (IIS) in organization. ... Journal of Research in National Development ... This research contributes to the field of Information Systems specifically in the area of Integrated Information System ...

  19. Partial purification of the 5-hydroxytryptophan-reuptake system from human blood platelets using a citalopram-derived affinity resin

    Energy Technology Data Exchange (ETDEWEB)

    Biessen, E.A.L; Horn, A.S.; Robillard, G.T. (Univ. of Groningen (Netherlands))

    1990-04-03

    This paper describes a procedure for the synthesis and application of a citalopram-derived affinity resin in purifying the 5HT-reuptake system from human blood platelets. A two-step scheme has been developed for partial purification, based on wheat germ agglutinin-lectin (WGA) affinity and citalopram affinity chromatographies. Upon solubilization of the carrier with 1% digitonin, a 50-70-fold increase in specific ({sup 3}H) imipramine binding activity with a 70% recovery could be accomplished through WGA-lectin chromatography. The WGA pool was then subjected to affinity chromatography on citalopram-agarose. At least 90% of the binding capacity adsorbed to the column. Specific elution using 10 {mu}M citalopram resulted in a 22% recovery of binding activity. A 10,000-fold overall purification was obtained by using this two-step procedure. Analysis of the fractions on SDS-PAGE after {sup 125}I labeling revealed specific elution of 78- and 55-kDa proteins concomitant with the appearance of ({sup 3}H) imipramine binding activity. The pharmacological profile of the partially purified reuptake system correlated well with that derived from the crude membrane-bound reuptake system, suggesting a copurification of the 5HT binding activity and ({sup 3}H)imipramine binding activity.

  20. Disposable on-chip microfluidic system for buccal cell lysis, DNA purification, and polymerase chain reaction.

    Science.gov (United States)

    Cho, Woong; Maeng, Joon-Ho; Ahn, Yoomin; Hwang, Seung Yong

    2013-09-01

    This paper reports the development of a disposable, integrated biochip for DNA sample preparation and PCR. The hybrid biochip (25 × 45 mm) is composed of a disposable PDMS layer with a microchannel chamber and reusable glass substrate integrated with a microheater and thermal microsensor. Lysis, purification, and PCR can be performed sequentially on this microfluidic device. Cell lysis is achieved by heat and purification is performed by mechanical filtration. Passive check valves are integrated to enable sample preparation and PCR in a fixed sequence. Reactor temperature is needed to lysis and PCR reaction is controlled within ±1°C by PID controller of LabVIEW software. Buccal epithelial cell lysis, DNA purification, and SY158 gene PCR amplification were successfully performed on this novel chip. Our experiments confirm that the entire process, except the off-chip gel electrophoresis, requires only approximately 1 h for completion. This disposable microfluidic chip for sample preparation and PCR can be easily united with other technologies to realize a fully integrated DNA chip.

  1. Biogas recirculation for simultaneous calcium removal and biogas purification within an expanded granular sludge bed system treating leachate.

    Science.gov (United States)

    Luo, Jinghuan; Lu, Xueqin; Liu, Jianyong; Qian, Guangren; Lu, Yongsheng

    2014-12-01

    Biogas, generated from an expanded granular sludge bed (EGSB) reactor treating municipal solid waste (MSW) leachate, was recirculated for calcium removal from the leachate via a carbonation process with simultaneous biogas purification. Batch trials were performed to optimize the solution pH and imported biogas (CO2) for CaCO3 precipitation. With applicable pH of 10-11 obtained, continuous trials achieved final calcium concentrations of 181-375 mg/L (removal efficiencies≈92.8-96.5%) in the leachate and methane contents of 87.1-91.4% (purification efficiencies≈65.4-82.2%) in the biogas. Calcium-balance study indicates that 23-986 mg Ca/d was released from the bio-system under the carbonized condition where CaCO3 precipitating was moved outside the bioreactor, whereas 7918-9517 mg Ca/d was trapped into the system for the controlled one. These findings demonstrate that carbonation removal of calcium by biogas recirculation could be a promising alternative to pretreat calcium-rich MSW leachate and synergistically to improve methane content.

  2. A Hybrid MPC-PID Control System Design for the Continuous Purification and Processing of Active Pharmaceutical Ingredients

    Directory of Open Access Journals (Sweden)

    Maitraye Sen

    2014-05-01

    Full Text Available In this work, a hybrid MPC (model predictive control-PID (proportional-integral-derivative control system has been designed for the continuous purification and processing framework of active pharmaceutical ingredients (APIs. The specific unit operations associated with the purification and processing of API have been developed from first-principles and connected in a continuous framework in the form of a flowsheet model. These integrated unit operations are highly interactive along with the presence of process delays. Therefore, a hybrid MPC-PID is a promising alternative to achieve the desired control loop performance as mandated by the regulatory authorities. The integrated flowsheet model has been simulated in gPROMSTM (Process System Enterprise, London, UK. This flowsheet model has been linearized in order to design the control scheme. The ability to track the set point and reject disturbances has been evaluated. A comparative study between the performance of the hybrid MPC-PID and a PID-only control scheme has been presented. The results show that an enhanced control loop performance can be obtained under the hybrid control scheme and demonstrate that such a scheme has high potential in improving the efficiency of pharmaceutical manufacturing operations.

  3. Use of Strep-tag II for rapid detection and purification of Mycobacterium tuberculosis recombinant antigens secreted by Streptomyces lividans.

    Science.gov (United States)

    Ayala, Julio C; Pimienta, Elsa; Rodríguez, Caridad; Anné, Jozef; Vallín, Carlos; Milanés, María T; King-Batsios, Emmanuel; Huygen, Kris; Van Mellaert, Lieve

    2013-09-01

    Recent results with respect to the secretory production of bio-active Mycobacterium tuberculosis proteins in Streptomyces have stimulated the further exploitation of this host as a bacterial cell factory. However, the rapid isolation of a recombinant protein by conventional procedures can be a restrictive step. A previous attempt to isolate recombinant antigens fused to the widely used 6His-tag was found to be relatively incompatible with secretory production in the Streptomyces host. As an alternative, the eight-residue Strep-tag® II (WSHPQFEK), displaying intrinsic binding affinity towards streptavidin, was evaluated for the secretory production of two M. tuberculosis immunodominant antigens in Streptomyces lividans and their subsequent downstream processing. Therefore, the genes ag85A (Rv3804c, encoding the mycolyl-transferase Ag85A) and Rv2626c (encoding hypoxic response protein 1), were equipped with a 3'-Strep-tag® II-encoding sequence and placed under control of the Streptomyces venezuelae CBS762.70 subtilisin inhibitor (vsi) transcriptional, translational and signal sequences. Strep-tagged Ag85A and Rv2626c proteins were detected in the spent medium of recombinant S. lividans cultures at 48h of growth, and purified using a Strep-Tactin Superflow® matrix. Recombinant Ag85A appeared as a 30-kDa protein of which the N-terminal amino acid sequence was identical to the expected one. Rv2626c was produced in two forms of 17 and 37kDa respectively, both with the same predicted N-terminal sequence, suggesting that the 37-kDa product is an Rv2626c dimer. The obtained results indicate that the Strep-tagII is proteolytically stable in Streptomyces and does not interfere with the membrane translocation of Ag85A and Rv2626c. A comparison of reactivity of serum from tuberculosis patients versus healthy persons by ELISA showed that both S. lividans-derived antigens were recognized by sera of individuals infected with M. tuberculosis, indicating that they remained

  4. Greedy Snake Video Game Based on Nios II System

    OpenAIRE

    2014-01-01

    With the development of large scale integrated circuit, traditional embedded system design method cannot meet the requirements for building complex systems. Therefore, SOC and SOPC technology are widely used nowadays. This thesis aims to development a game platform based on Nios II system using Altera DE2 development and education board and SOPC technology. In this thesis, first a custom Nios II system was built according to the requirements of the game application. The system contains hardwa...

  5. Improvement of the Purification System of Scrubbing Tower%洗涤塔净化系统的改进

    Institute of Scientific and Technical Information of China (English)

    赵英群

    2013-01-01

    详细介绍了本钢5#高炉煤气净化系统的老洗涤工艺与生产中的不利因素,根据生产的需要对排水系统和液压控制系统进行了改进,新改进的系统在实际应用中相比原系统提高了工作效率。%The negative factors in the original scrubbing process and production of the gas purification system of Benxi Steel’s No.5 blast furnace are introduced. The water drainage and hydraulic control systems were renovated according to the requirement of production; and the improved systems performed more efficiently compared to the old ones.

  6. A Genetically Tagged Psb27 Protein Allows Purification of Two Consecutive Photosystem II (PSII) Assembly Intermediates in Synechocystis 6803, a Cyanobacterium*

    Science.gov (United States)

    Liu, Haijun; Roose, Johnna L.; Cameron, Jeffrey C.; Pakrasi, Himadri B.

    2011-01-01

    Photosystem II (PSII) is a large membrane bound molecular machine that catalyzes light-driven oxygen evolution from water. PSII constantly undergoes assembly and disassembly because of the unavoidable damage that results from its normal photochemistry. Thus, under physiological conditions, in addition to the active PSII complexes, there are always PSII subpopulations incompetent of oxygen evolution, but are in the process of undergoing elaborate biogenesis and repair. These transient complexes are difficult to characterize because of their low abundance, structural heterogeneity, and thermodynamic instability. In this study, we show that a genetically tagged Psb27 protein allows for the biochemical purification of two monomeric PSII assembly intermediates, one with an unprocessed form of D1 (His27ΔctpAPSII) and a second one with a mature form of D1 (His27PSII). Both forms were capable of light-induced charge separation, but unable to photooxidize water, largely because of the absence of a functional tetramanganese cluster. Unexpectedly, there was a significant amount of the extrinsic lumenal PsbO protein in the His27PSII, but not in the His27ΔctpAPSII complex. In contrast, two other lumenal proteins, PsbU and PsbV, were absent in both of these PSII intermediate complexes. Additionally, the only cytoplasmic extrinsic protein, Psb28 was detected in His27PSII complex. Based on these data, we have presented a refined model of PSII biogenesis, illustrating an important role of Psb27 as a gate-keeper during the complex assembly process of the oxygen-evolving centers in PSII. PMID:21592967

  7. Telomerase repeat amplification protocol (TRAP) activity upon recombinant expression and purification of human telomerase in a bacterial system.

    Science.gov (United States)

    Hansen, Debra T; Thiyagarajan, Thirumagal; Larson, Amy C; Hansen, Jeffrey L

    2016-07-01

    Telomerase biogenesis is a highly regulated process that solves the DNA end-replication problem. Recombinant expression has so far been accomplished only within a eukaryotic background. Towards structural and functional analyses, we developed bacterial expression of human telomerase. Positive activity by the telomerase repeat amplification protocol (TRAP) was identified in cell extracts of Escherichia coli expressing a sequence-optimized hTERT gene, the full-length hTR RNA with a self-splicing hepatitis delta virus ribozyme, and the human heat shock complex of Hsp90, Hsp70, p60/Hop, Hsp40, and p23. The Hsp90 inhibitor geldanamycin did not affect post-assembly TRAP activity. By various purification methods, TRAP activity was also obtained upon expression of only hTERT and hTR. hTERT was confirmed by tandem mass spectrometry in a ∼120 kDa SDS-PAGE fragment from a TRAP-positive purification fraction. TRAP activity was also supported by hTR constructs lacking the box H/ACA small nucleolar RNA domain. End-point TRAP indicated expression levels within 3-fold of that from HeLa carcinoma cells, which is several orders of magnitude below detection by the direct assay. These results represent the first report of TRAP activity from a bacterium and provide a facile system for the investigation of assembly factors and anti-cancer therapeutics independently of a eukaryotic setting.

  8. Purification and characterization of the plasmodial phosphatase that hydrolyses the phosphorylated light chain of Physarum myosin II from Physarum polycephalum.

    Science.gov (United States)

    Okada, Chisa; Nakamura, Akio; Tomioka, Shigeo; Kohama, Kazuhiro; Kaneko, Takako S

    2010-08-01

    A phosphatase was purified through a combination of ion-exchange and hydrophobic chromatography followed by native PAGE from Physarum plasmodia. Recently, we demonstrated that this phosphatase isoform has a hydrolytic activity towards the PMLC (phosphorylated light chain of Physarum myosin II) at pH 7.6. The apparent molecular mass of the purified enzyme was estimated at approximately 50 kDa by means of analytical gel filtration. The enzyme was purified 340-fold to a final phosphatase activity of 400 pkat/mg of protein. Among the phosphorylated compounds tested for hydrolytic activity at pH 7.6, the enzyme showed no activity towards nucleotides. At pH 7.6, hydrolytic activity of the enzyme against PMLC was detected; at pH 5.0, however, no hydrolytic activity towards PMLC was observed. The Km of the enzyme for PMLC was 10 microM, and the V(max) was 1.17 nkat/mg of protein. Ca(2+) (10 microM) inhibited the activity of the enzyme, and Mg(2+) (8.5 microM) activated the dephosphorylation of PMLC. Mn(2+) (1.6 microM) highly stimulated the enzyme's activity. Based on these results, we concluded that the enzyme is likely to be a phosphatase with hydrolytic activity towards PMLC.

  9. Expression and purification of the matrix protein of Nipah virus in baculovirus insect cell system.

    Science.gov (United States)

    Masoomi Dezfooli, Seyedehsara; Tan, Wen Siang; Tey, Beng Ti; Ooi, Chien Wei; Hussain, Siti Aslina

    2016-01-01

    Nipah virus (NiV) causes fatal respiratory illness and encephalitis in humans and animals. The matrix (M) protein of NiV plays an important role in the viral assembly and budding process. Thus, an access to the NiV M protein is vital to the design of viral antigens as diagnostic reagents. In this study, recombinant DNA technology was successfully adopted in the cloning and expression of NiV M protein. A recombinant expression cassette (baculovirus expression vector) was used to encode an N-terminally His-tagged NiV M protein in insect cells. A time-course study demonstrated that the highest yield of recombinant M protein (400-500 μg) was expressed from 107 infected cells 3 days after infection. A single-step purification method based on metal ion affinity chromatography was established to purify the NiV M protein, which successfully yielded a purity level of 95.67% and a purification factor of 3.39. The Western blotting and enzyme-linked immunosorbent assay (ELISA) showed that the purified recombinant M protein (48 kDa) was antigenic and reacted strongly with the serum of a NiV infected pig.

  10. Present status of the TJ-II remote participation system

    Energy Technology Data Exchange (ETDEWEB)

    Vega, J. [Asociacion EURATOM/CIEMAT para Fusion., Avda. Complutense 22, 28040 Madrid (Spain)]. E-mail: jesus.vega@ciemat.es; Sanchez, E. [Asociacion EURATOM/CIEMAT para Fusion., Avda. Complutense 22, 28040 Madrid (Spain); Lopez, A. [Asociacion EURATOM/CIEMAT para Fusion., Avda. Complutense 22, 28040 Madrid (Spain); Portas, A. [Asociacion EURATOM/CIEMAT para Fusion., Avda. Complutense 22, 28040 Madrid (Spain); Ochando, M. [Asociacion EURATOM/CIEMAT para Fusion., Avda. Complutense 22, 28040 Madrid (Spain); Ascasibar, E. [Asociacion EURATOM/CIEMAT para Fusion., Avda. Complutense 22, 28040 Madrid (Spain); Mollinedo, A. [CIEMAT. Computing Center, Avda. Complutense 22, 28040 Madrid (Spain); Munoz, J. [CIEMAT. Computing Center, Avda. Complutense 22, 28040 Madrid (Spain); Sanchez, A. [CIEMAT. Computing Center, Avda. Complutense 22, 28040 Madrid (Spain); Ruiz, M. [Universidad Politecnica de Madrid. Dpto. Sistemas Electronicos y de Control, Campus Sur. Ctra. Valencia, km 7, 28031 Madrid (Spain); Barrera, E. [Universidad Politecnica de Madrid. Dpto. Sistemas Electronicos y de Control, Campus Sur. Ctra. Valencia, km 7, 28031 Madrid (Spain); Lopez, S. [Universidad Politecnica de Madrid. Dpto. Sistemas Electronicos y de Control, Campus Sur. Ctra. Valencia, km 7, 28031 Madrid (Spain); Castro, R. [Red.es-RedIRIS, Edificio Bronce, Plaza Manuel Gomez Moreno, s/n, 28020 Madrid (Spain); Lopez, D. [Red.es-RedIRIS, Edificio Bronce, Plaza Manuel Gomez Moreno, s/n, 28020 Madrid (Spain)

    2005-11-15

    The TJ-II remote participation system (RPS) was designed to extend to Internet the working capabilities provided in the TJ-II local environment, i.e., tracking the TJ-II operation, monitoring/programming data acquisition and control systems, and accessing databases. The TJ-II RPS was based on web and Java technologies because of their open character, security properties and technological maturity. A web server acts as a communication front-end between remote participants and local TJ-II elements. From the server side, web services are provided by means of resources supplied by JSP pages. The client part makes use of web browsers and ad hoc Java applications. The operation requires the use of a distributed authentication and authorization system. This development employs the PAPI System. At present, approximately 1000 digitisation channels can be managed from the TJ-II RPS. Furthermore, processing software based on a 4GL language (LabView) can be downloaded to multiprocessor data acquisition systems. Also, 15 diagnostic control systems, databases and the operation logbook are available from the RPS. The system even allows for the physicist in charge of operation to be in a remote location. Four Spanish universities make use of the TJ-II remote participation system capabilities for joint collaborations: these are the Universidad Politecnica de Madrid (UPM), Universidad Nacional de Educacion a Distancia (UNED), Universidad Complutense de Madrid (UCM) and Universidad Politecnica de Cataluna (UPC)

  11. HPC Colony II: FAST_OS II: Operating Systems and Runtime Systems at Extreme Scale

    Energy Technology Data Exchange (ETDEWEB)

    Moreira, Jose [IBM, Armonk, NY (United States)

    2013-11-13

    HPC Colony II has been a 36-month project focused on providing portable performance for leadership class machines—a task made difficult by the emerging variety of more complex computer architectures. The project attempts to move the burden of portable performance to adaptive system software, thereby allowing domain scientists to concentrate on their field rather than the fine details of a new leadership class machine. To accomplish our goals, we focused on adding intelligence into the system software stack. Our revised components include: new techniques to address OS jitter; new techniques to dynamically address load imbalances; new techniques to map resources according to architectural subtleties and application dynamic behavior; new techniques to dramatically improve the performance of checkpoint-restart; and new techniques to address membership service issues at scale.

  12. The influence of molecular weight of polyethylene glycol on separation and purification of pectinases from Penicillium cyclopium in aqueous two-phase system

    Directory of Open Access Journals (Sweden)

    Prodanović Jelena M.

    2008-01-01

    Full Text Available In this study the possibility of the partitioning and purification of pectinases from Penicillium cyclopium by their partitioning in polymer/polymer and polymer/salt aqueous two-phase systems was investigated. In the system with 10% (w/w polyethylene glycol 1500/5% (w/w dextran 500 000/85% (w/w crude enzyme, the highest values for partitioning parameters were achieved - the partition coefficient was 2.11, followed by the top phase yield of 85.68% and purification factor 1.28 for the endo-pectinase activity. The partition coefficient, yield in the top phase and purification factor for the exo-pectinase activity in the same system were 1.89, 84.28% and 3.82, respectively. In the system with 10% (w/w polyethylene glycol 6000/15% (w/w (NH42SO4/75% (w/w crude enzyme purification factor 37.85 for exo-pectinase, and 19.52 for endo-pectinase in the bottom phase were obtained.

  13. Direct Purification of Pectinase from Mango (Mangifera Indica Cv. Chokanan Peel Using a PEG/Salt-Based Aqueous Two Phase System

    Directory of Open Access Journals (Sweden)

    Abdul Manap Mohd Yazid

    2011-10-01

    Full Text Available An Aqueous Two-Phase System (ATPS was employed for the first time for the separation and purification of pectinase from mango (Mangifera Indica Cv. Chokanan peel. The effects of different parameters such as molecular weight of the polymer (polyethylene glycol, 2,000–10,000, potassium phosphate composition (12–20%, w/w, system pH (6–9, and addition of different concentrations of neutral salts (0–8%, w/w on partition behavior of pectinase were investigated. The partition coefficient of the enzyme was decreased by increasing the PEG molecular weight. Additionally, the phase composition showed a significant effect on purification factor and yield of the enzyme. Optimum conditions for purification of pectinase from mango peel were achieved in a 14% PEG 4000-14% potassium phosphate system using 3% (w/w NaCl addition at pH 7.0. Based on this system, the purification factor of pectinase was increased to 13.2 with a high yield of (97.6%. Thus, this study proves that ATPS can be an inexpensive and effective method for partitioning of pectinase from mango peel.

  14. Direct purification of pectinase from mango (Mangifera Indica Cv. Chokanan) peel using a PEG/salt-based Aqueous Two Phase System.

    Science.gov (United States)

    Mehrnoush, Amid; Sarker, Md Zaidul Islam; Mustafa, Shuhaimi; Yazid, Abdul Manap Mohd

    2011-10-10

    An Aqueous Two-Phase System (ATPS) was employed for the first time for the separation and purification of pectinase from mango (Mangifera Indica Cv. Chokanan) peel. The effects of different parameters such as molecular weight of the polymer (polyethylene glycol, 2,000-10,000), potassium phosphate composition (12-20%, w/w), system pH (6-9), and addition of different concentrations of neutral salts (0-8%, w/w) on partition behavior of pectinase were investigated. The partition coefficient of the enzyme was decreased by increasing the PEG molecular weight. Additionally, the phase composition showed a significant effect on purification factor and yield of the enzyme. Optimum conditions for purification of pectinase from mango peel were achieved in a 14% PEG 4000-14% potassium phosphate system using 3% (w/w) NaCl addition at pH 7.0. Based on this system, the purification factor of pectinase was increased to 13.2 with a high yield of (97.6%). Thus, this study proves that ATPS can be an inexpensive and effective method for partitioning of pectinase from mango peel.

  15. Evaluation of the Quantum II yeast identification system.

    OpenAIRE

    Kiehn, T E; Edwards, F F; Tom, D; LIEBERMAN, G; Bernard, E M; Armstrong, D.

    1985-01-01

    We compared three methods for identifying clinical yeast isolates: Abbott Quantum II, API 20C, and a modified BBL Minitek system. The API 20C and modified Minitek systems agreed on the identification of 243 of 245 yeasts (99.2%). The Quantum II system correctly identified 197 (80.4%), incorrectly identified 19 (7.8%), and did not identify 29 (11.8%) of the yeasts. Most of the misidentifications with the Quantum II occurred because assimilation or biochemical results were false-positive. Sixte...

  16. Optimization of serine protease purification from mango (Mangifera indica cv. Chokanan) peel in polyethylene glycol/dextran aqueous two phase system.

    Science.gov (United States)

    Mehrnoush, Amid; Mustafa, Shuhaimi; Sarker, Md Zaidul Islam; Yazid, Abdul Manap Mohd

    2012-01-01

    Mango peel is a good source of protease but remains an industrial waste. This study focuses on the optimization of polyethylene glycol (PEG)/dextran-based aqueous two-phase system (ATPS) to purify serine protease from mango peel. The activity of serine protease in different phase systems was studied and then the possible relationship between the purification variables, namely polyethylene glycol molecular weight (PEG, 4000-12,000 g·mol(-1)), tie line length (-3.42-35.27%), NaCl (-2.5-11.5%) and pH (4.5-10.5) on the enzymatic properties of purified enzyme was investigated. The most significant effect of PEG was on the efficiency of serine protease purification. Also, there was a significant increase in the partition coefficient with the addition of 4.5% of NaCl to the system. This could be due to the high hydrophobicity of serine protease compared to protein contaminates. The optimum conditions to achieve high partition coefficient (84.2) purification factor (14.37) and yield (97.3%) of serine protease were obtained in the presence of 8000 g·mol(-1) of PEG, 17.2% of tie line length and 4.5% of NaCl at pH 7.5. The enzymatic properties of purified serine protease using PEG/dextran ATPS showed that the enzyme could be purified at a high purification factor and yield with easy scale-up and fast processing.

  17. Enhancement of a novel extracellular uricase production by media optimization and partial purification by aqueous three-phase system.

    Science.gov (United States)

    Ram, Senthoor K; Raval, Keyur; JagadeeshBabu, P E

    2015-01-01

    Uricase (urate: oxygen oxidoreductase, EC 1.7.3.3), an enzyme belonging to the class of oxidoreductases, catalyzes the enzymatic oxidation of uric acid to allantoin and finds a wide variety of application as therapeutic and clinical reagent. In this study, uricase production ability of the bacterial strains isolated from deep litter poultry soil is investigated. The strain with maximum extracellular uricase production capability was identified as Xanthomonas fuscans subsp. aurantifolii based on 16S rRNA sequencing. Effect of various carbon and nitrogen sources on uricase productivity was investigated. The uricase production for this strain was optimized using statistically based experimental designs and resulted in uricase activity of 306 U/L, which is 2 times higher than initial uricase activity. Two-step purification, such as ammonium sulfate precipitation and aqueous two-phase system, was carried out and a twofold increase in yield and specific activity was observed.

  18. Numerical studies of an eccentric tube-in-tube helically coiled heat exchanger for IHEP-ADS helium purification system

    CERN Document Server

    Zhang, Jianqin

    2014-01-01

    The tube-in-tube helically coiled (TTHC) heat exchanger is preferred in the purifier of IHEP-ADS helium purification system. The position of an internal tube is usually eccentric in a TTHC heat exchanger in practice, while most TTHC heat exchangers in the literature studied are concentric. In this paper, TTHC heat exchangers with different eccentricity ratios are numerically studied for turbulent flow and heat transfer characteristics under different flow rates. The fluid considered is helium at the pressure of 20Mpa, with temperature dependent thermo-physical properties for the inner tube and the annulus. The inner Nusselt number between the concentric and eccentric TTHC heat exchangers are compared, so is the annulus Nusselt number. The results show that with the eccentricity increasing, the annulus Nusselt number increases substantially. According to the numerical data, new empirical correlations of Nusselt number as a function of Reynolds number and eccentricity for the inner tube and the annulus are pres...

  19. Method and system for purification of gas/liquid streams for fuel cells or electrolysis cells

    DEFF Research Database (Denmark)

    2013-01-01

    The present invention provides in embodiments a method for purification of inlet gas/liquid streams in a fuel cell or electrolysis cell, the fuel cell or electrolysis cell comprising at least a first electrode, an electrolyte and a second electrode, the method comprising the steps of: - providing...... at least one scrubber in the gas/liquid stream at the inlet side of the first electrode of the fuel cell or electrolysis cell; and/or providing at least one scrubber in the gas/liquid stream at the inlet side of the second electrode of the fuel cell or electrolysis cell; and - purifying the gas...... with the at least one scrubber, with the proviso that the fuel cell or electrolysis cell is not a solid oxide cell....

  20. The Population of Weak MgII Absorbers. II The Properties of Single-Cloud Systems

    CERN Document Server

    Rigby, J R; Churchill, C W; Rigby, Jane R.; Charlton, Jane C.; Churchill, Christopher W.

    2001-01-01

    We present an investigation of MgII absorbers characterized as single-cloud weak systems at z~1. We measured column densities and Doppler parameters for MgII and FeII in 15 systems found in HIRES/Keck spectra at 6.6 km/s. Using these quantities and CIV, Lyman alpha and Lyman limit absorption observed with FOS/HST (resolution ~230 km/s) we applied photoionization models to each system to constrain metallicities, densities, ionization conditions, and sizes. We find that: (1) Single-cloud weak systems are optically thin in neutral hydrogen and may have their origins in a population of objects distinct from the optically thick strong MgII absorbers, which are associated with bright galaxies. (2) Weak systems account for somewhere between 25% to 100% of the z < 1 Lyman alpha forest clouds in the range 15.8systems have two or more ionization phases of gas (multiphase medium). (4) We identify a subset of weak MgII absorber that we term ``iron-rich''. These clo...

  1. Purification of pectinase from mango (Mangifera indica L. cv. Chokanan) waste using an aqueous organic phase system: a potential low cost source of the enzyme.

    Science.gov (United States)

    Amid, Mehrnoush; Abdul Manap, Mohd Yazid; Mustafa, Shuhaimi

    2013-07-15

    As a novel method of purification, an aqueous organic phase system (AOPS) was employed to purify pectinase from mango waste. The effect of different parameters, such as the alcohol concentration (ethanol, 1-propanol, and 2-propanol), the salt type and concentration (ammonium sulfate, potassium phosphate and sodium citrate), the feed stock crude load, the aqueous phase pH and NaCl concentration, were investigated in the recovery of pectinase from mango peel. The partition coefficient (K), selectivity (S), purification factor (PF) and yield (Y, %) were investigated in this study as important parameters for the evaluation of enzyme recovery. The desirable partition efficiency for pectinase purification was achieved in an AOPS of 19% (w/w) ethanol and 22% (w/w) potassium phosphate in the presence of 5% (w/w) NaCl at pH 7.0. Based on the system, the purification factor of pectinase was enhanced 11.7, with a high yield of 97.1%. Copyright © 2013 Elsevier B.V. All rights reserved.

  2. Rapid, highly efficient extraction and purification of membrane proteins using a microfluidic continuous-flow based aqueous two-phase system.

    Science.gov (United States)

    Hu, Rui; Feng, Xiaojun; Chen, Pu; Fu, Meng; Chen, Hong; Guo, Lin; Liu, Bi-Feng

    2011-01-07

    Membrane proteins play essential roles in regulating various fundamental cellular functions. To investigate membrane proteins, extraction and purification are usually prerequisite steps. Here, we demonstrated a microfluidic aqueous PEG/detergent two-phase system for the purification of membrane proteins from crude cell extract, which replaced the conventional discontinuous agitation method with continuous extraction in laminar flows, resulting in significantly increased extraction speed and efficiency. To evaluate this system, different separation and detection methods were used to identify the purified proteins, such as capillary electrophoresis, SDS-PAGE and nano-HPLC-MS/MS. Swiss-Prot database with Mascot search engine was used to search for membrane proteins from random selected bands of SDS-PAGE. Results indicated that efficient purification of membrane proteins can be achieved within 5-7s and approximately 90% of the purified proteins were membrane proteins (the highest extraction efficiency reported up to date), including membrane-associated proteins and integral membrane proteins with multiple transmembrane domains. Compared to conventional approaches, this new method had advantages of greater specific surface area, minimal emulsification, reduced sample consumption and analysis time. We expect the developed method to be potentially useful in membrane protein purifications, facilitating the investigation of membrane proteomics.

  3. The Muon system of the run II D0 detector

    Energy Technology Data Exchange (ETDEWEB)

    Abazov, V.M.; Acharya, B.S.; Alexeev, G.D.; Alkhazov, G.; Anosov, V.A.; Baldin, B.; Banerjee, S.; Bardon, O.; Bartlett, J.F.; Baturitsky, M.A.; Beutel, D.; Bezzubov,; Bodyagin, V.; Butler, J.M.; Cease, H.; Chi, E.; Denisov, D.; Denisov, S.P.; Diehl, H.T.; Doulas, S.; Dugad, S.R.; /Beijing, Inst. High Energy Phys. /Charles U. /Prague, Tech.

    2005-03-01

    The authors describe the design, construction and performance of the upgraded D0 muon system for Run II of the Fermilab Tevatron collider. Significant improvements have been made to the major subsystems of the D0 muon detector: trigger scintillation counters, tracking detectors, and electronics. The Run II central muon detector has a new scintillation counter system inside the iron toroid and an improved scintillation counter system outside the iron toroid. In the forward region, new scintillation counter and tracking systems have been installed. Extensive shielding has been added in the forward region. A large fraction of the muon system electronics is also new.

  4. Alternative pathways for angiotensin II generation in the cardiovascular system

    Directory of Open Access Journals (Sweden)

    C. Becari

    2011-09-01

    Full Text Available The classical renin-angiotensin system (RAS consists of enzymes and peptides that regulate blood pressure and electrolyte and fluid homeostasis. Angiotensin II (Ang II is one of the most important and extensively studied components of the RAS. The beneficial effects of angiotensin converting enzyme (ACE inhibitors in the treatment of hypertension and heart failure, among other diseases, are well known. However, it has been reported that patients chronically treated with effective doses of these inhibitors do not show suppression of Ang II formation, suggesting the involvement of pathways alternative to ACE in the generation of Ang II. Moreover, the finding that the concentration of Ang II is preserved in the kidney, heart and lungs of mice with an ACE deletion indicates the important role of alternative pathways under basal conditions to maintain the levels of Ang II. Our group has characterized the serine protease elastase-2 as an alternative pathway for Ang II generation from Ang I in rats. A role for elastase-2 in the cardiovascular system was suggested by studies performed in heart and conductance and resistance vessels of normotensive and spontaneously hypertensive rats. This mini-review will highlight the pharmacological aspects of the RAS, emphasizing the role of elastase-2, an alternative pathway for Ang II generation.

  5. Argus phase II optical data collection system

    Science.gov (United States)

    Wasson, Wayne E.

    1996-11-01

    The Argus aircraft is a highly modified NC-135E fitted with an infrared and ultraviolet-visible sensor suite for radiometric and spectral data collection. Each suite is operated independently with its own separate gimbal for precision pointing, telescope, and relay optics. The system includes a silica window for the ultraviolet-visible, and a zinc selenide window for the infrared. The entire system was developed and fabricated in-house at the Phillips Laboratory. All sensors are calibrated as a system onboard the aircraft through a unique facility called the aircraft optical calibration facility. The data is all recorded digitally, and can be transferred to secure data reduction facilities via optical fiber. The system is modular, in that the ultraviolet-visible and infrared benches can be separated, or the entire system can be quickly removed to allow for the introduction of other sensor suites or systems. The gimbals and telescopes can be used independently of the rest of the system. The aircraft is also fitted with an anemometry system, which can be operated independently of the sensor systems. This aircraft is capable of many types of missions, and will soon be fitted with a LIDAR system for remote sensing. The philosophy in building the system is to make it capable of quick changes during mission.

  6. Ionic Liquid-Based Ultrasonic-Assisted Extraction of Secoisolariciresinol Diglucoside from Flaxseed (Linum usitatissimum L.) with Further Purification by an Aqueous Two-Phase System.

    Science.gov (United States)

    Tan, Zhi-Jian; Wang, Chao-Yun; Yang, Zi-Zhen; Yi, Yong-Jian; Wang, Hong-Ying; Zhou, Wan-Lai; Li, Fen-Fang

    2015-09-30

    In this work, a two-step extraction methodology of ionic liquid-based ultrasonic-assisted extraction (IL-UAE) and ionic liquid-based aqueous two-phase system (IL-ATPS) was developed for the extraction and purification of secoisolariciresinol diglucoside (SDG) from flaxseed. In the IL-UAE step, several kinds of ILs were investigated as the extractants, to identify the IL that affords the optimum extraction yield. The extraction conditions such as IL concentration, ultrasonic irradiation time, and liquid-solid ratio were optimized using response surface methodology (RSM). In the IL-ATPS step, ATPS formed by adding kosmotropic salts to the IL extract was used for further separation and purification of SDG. The most influential parameters (type and concentration of salt, temperature, and pH) were investigated to obtain the optimum extraction efficiency. The maximum extraction efficiency was 93.35% under the optimal conditions of 45.86% (w/w) IL and 8.27% (w/w) Na₂SO₄ at 22 °C and pH 11.0. Thus, the combination of IL-UAE and IL-ATPS makes up a simple and effective methodology for the extraction and purification of SDG. This process is also expected to be highly useful for the extraction and purification of bioactive compounds from other important medicinal plants.

  7. Method and system for purification of gas/liquid streams for fuel cells or electrolysis cells

    DEFF Research Database (Denmark)

    2013-01-01

    The present invention provides in embodiments a method for purification of inlet gas/liquid streams in a fuel cell or electrolysis cell, the fuel cell or electrolysis cell comprising at least a first electrode, an electrolyte and a second electrode, the method comprising the steps of: - providing...... at least one scrubber in the gas/liquid stream at the inlet side of the first electrode of the fuel cell or electrolysis cell; and/or providing at least one scrubber in the gas/liquid stream at the inlet side of the second electrode of the fuel cell or electrolysis cell; and - purifying the gas/liquid...... streams towards the first and second electrode; wherein the at least one scrubber in the gas/liquid stream at the inlet side of the first electrode and/or the at least one scrubber in the gas/liquid stream at the inlet side of the second electrode comprises a material suitable as an electrolyte material...

  8. The Bacillus cereus spoIIS programmed cell death system

    Directory of Open Access Journals (Sweden)

    Jana eMelnicakova

    2015-08-01

    Full Text Available Programmed cell death in bacteria is generally associated with two¬ component toxin antitoxin systems. The SpoIIS toxin-antitoxin system, consisting of a membrane bound SpoIISA toxin and a small, cytosolic antitoxin SpoIISB, was originally identified in Bacillus subtilis. In this work we describe the Bacillus cereus SpoIIS system which is a three-component system, harbouring an additional gene spoIISC. Its protein product serves as an antitoxin, and similarly as SpoIISB, is able to bind SpoIISA and abolish its toxic effect. Our results indicate that SpoIISC seems to be present not only in B. cereus but also in other Bacilli containing a SpoIIS toxin antitoxin system. In addition, we show that B. cereus SpoIISA can form higher oligomers and we discuss the possible role of this multimerization for the protein’s toxic function.

  9. An AIL/IL-based liquid/liquid extraction system for the purification of His-tagged proteins.

    Science.gov (United States)

    Xu, Weiyuan; Cao, Huazhen; Ren, Guangwei; Xie, Hujun; Huang, Jianying; Li, Shijun

    2014-06-01

    A sorbent based on affinity ionic liquid (AIL), triazacyclononane-ionic liquid, was synthesized, characterized, and applied to the extraction of histidine (His)-tagged proteins from aqueous buffer to ionic liquid (IL) phase. The adsorbed His-tagged proteins could be back-extracted from the IL phase to the aqueous buffer with an imidazole solution. The specific binding of His-tagged proteins with AIL/IL could be affected by a few factors including the ionic strength and coordinated metal ions. In the case of His-tagged enhanced green fluorescent protein (EGFP), the maximum binding capacity of Cu(2+)-AIL/IL reached 2.58 μg/μmol under the optimized adsorption conditions. The eluted His-tagged EGFP kept fluorescent and remained active through the purification process. Moreover, a tandem extraction process successively using Cu(2+)-AIL/IL and Zn(2+)-AIL/IL systems was developed, which was proven very efficient to obtain the ultimate protein with a purity of about 90 %. An effective reclamation method for the AIL/IL extraction system was further established. The sorbent could be easily regenerated by removing metal ions with EDTA and the followed reimmobilization of metal ions. Easy handling of the presented M(2+)-AIL/IL system and highly specific ability to absorb His-tagged proteins make it attractive and potentially applicable in biomolecular separation.

  10. General Motors Phase II Catalyst System

    Energy Technology Data Exchange (ETDEWEB)

    Canale, R.P.; Winegarden, S.R.; Carlson, C.R.; Miles, D.L.

    1978-01-01

    Three-way catalysts provide a means of catalytically achieving lower NOx emission levels while maintaining good control of HC and CO emissions. However, very accurate control of air-fuel ratio is necessary. The precise air-fuel ratio control required is accomplished by employing a closed loop fuel metering system in conjunction with an exhaust gas sensor and an electronic control unit. To gain production experience with this type of system, General Motors is introducing it on two 1978 engine families sold in California. One is a 2.5 liter L-4 engine and the other is a 3.8 liter V-6 engine. Closed loop controlled carburetors are used on both systems. The components used on both systems are described and emission and fuel economy results are reviewed.

  11. On Split Lie Triple Systems II

    Indian Academy of Sciences (India)

    Antonio J Calderón Martín; M Forero Piulestán

    2010-04-01

    In [4] it is studied that the structure of split Lie triple systems with a coherent 0-root space, that is, satisfying $[T_0,T_0,T]=0$ and $[T_0,T_,T_0]≠ 0$ for any nonzero root and where $T_0$ denotes the 0-root space and $T_$ the -root space, by showing that any of such triple systems with a symmetric root system is of the form $T=\\mathcal{U}+\\sum_j I_j$ with $\\mathcal{U}$ a subspace of the 0-root space $T_0$ and any $I_j$ a well described ideal of , satisfying $[I_j,T,I_k]=0$ if $j≠ k$. It is also shown in [4] that under certain conditions, a split Lie triple system with a coherent 0-root space is the direct sum of the family of its minimal ideals, each one being a simple split Lie triple system, and the simplicity of is characterized. In the present paper we extend these results to arbitrary split Lie triple systems with no restrictions on their 0-root spaces.

  12. A Novel Recombinant DNA System for High Efficiency Affinity Purification of Proteins in Saccharomyces cerevisiae

    Directory of Open Access Journals (Sweden)

    Brian H. Carrick

    2016-03-01

    Full Text Available Isolation of endogenous proteins from Saccharomyces cerevisiae has been facilitated by inserting encoding polypeptide affinity tags at the C-termini of chromosomal open reading frames (ORFs using homologous recombination of DNA fragments. Tagged protein isolation is limited by a number of factors, including high cost of affinity resins for bulk isolation and low concentration of ligands on the resin surface, leading to low isolation efficiencies and trapping of contaminants. To address this, we have created a recombinant “CelTag” DNA construct from which PCR fragments can be created to easily tag C-termini of S. cerevisiae ORFs using selection for a nat1 marker. The tag has a C-terminal cellulose binding module to be used in the first affinity step. Microgranular cellulose is very inexpensive and has an effectively continuous ligand on its surface, allowing rapid, highly efficient purification with minimal background. Cellulose-bound proteins are released by specific cleavage of an included site for TEV protease, giving nearly pure product. The tag can be lifted from the recombinant DNA construct either with or without a 13x myc epitope tag between the target ORF and the TEV protease site. Binding of CelTag protein fusions to cellulose is stable to high salt, nonionic detergents, and 1 M urea, allowing stringent washing conditions to remove loosely associated components, as needed, before specific elution. It is anticipated that this reagent could allow isolation of protein complexes from large quantities of yeast extract, including soluble, membrane-bound, or nucleic acid-associated assemblies.

  13. A Novel Recombinant DNA System for High Efficiency Affinity Purification of Proteins in Saccharomyces cerevisiae.

    Science.gov (United States)

    Carrick, Brian H; Hao, Linxuan; Smaldino, Philip J; Engelke, David R

    2015-12-29

    Isolation of endogenous proteins from Saccharomyces cerevisiae has been facilitated by inserting encoding polypeptide affinity tags at the C-termini of chromosomal open reading frames (ORFs) using homologous recombination of DNA fragments. Tagged protein isolation is limited by a number of factors, including high cost of affinity resins for bulk isolation and low concentration of ligands on the resin surface, leading to low isolation efficiencies and trapping of contaminants. To address this, we have created a recombinant "CelTag" DNA construct from which PCR fragments can be created to easily tag C-termini of S. cerevisiae ORFs using selection for a nat1 marker. The tag has a C-terminal cellulose binding module to be used in the first affinity step. Microgranular cellulose is very inexpensive and has an effectively continuous ligand on its surface, allowing rapid, highly efficient purification with minimal background. Cellulose-bound proteins are released by specific cleavage of an included site for TEV protease, giving nearly pure product. The tag can be lifted from the recombinant DNA construct either with or without a 13x myc epitope tag between the target ORF and the TEV protease site. Binding of CelTag protein fusions to cellulose is stable to high salt, nonionic detergents, and 1 M urea, allowing stringent washing conditions to remove loosely associated components, as needed, before specific elution. It is anticipated that this reagent could allow isolation of protein complexes from large quantities of yeast extract, including soluble, membrane-bound, or nucleic acid-associated assemblies.

  14. Handbook of networks in power systems II

    Energy Technology Data Exchange (ETDEWEB)

    Sorokin, Alexey; Pardalos, Panos M. [Florida Univ., Gainesville, FL (United States). Dept. of Industrial and Systems Engineering; Rebennack, Steffen [Colorado School of Mines, Golden, CO (United States). Div. of Economics and Business; Iliadis, Niko A. [EnerCoRD - Energy Consulting, Athens (Greece). Research and Development; Pereira, Mario V.F. (eds.) [Centro Empresarial, Rio de Janeiro (Brazil)

    2012-07-01

    Energy has been an inevitable component of human lives for decades. Recent rapid developments in the area require analyzing energy systems not as independent components but rather as connected interdependent networks. The Handbook of Networks in Power Systems includes the state-of-the-art developments that occurred in the power systems networks, in particular gas, electricity, liquid fuels, freight networks, as well as their interactions. The book is separated into two volumes with three sections, where one scientific paper or more are included to cover most important areas of networks in power systems. The first volume covers topics arising in electricity network, in particular electricity markets, smart grid, network expansion, as well as risk management. The second volume presents problems arising in gas networks; such as scheduling and planning of natural gas systems, pricing, as well as optimal location of gas supply units. In addition, the second volume covers the topics of interactions between energy networks. Each subject is identified following the activity on the domain and the recognition of each subject as an area of research. The scientific papers are authored by world specialists on the domain and present either state-of-the-arts reviews or scientific developments.

  15. Expression, stabilization and purification of membrane proteins via diverse protein synthesis systems and detergents involving cell-free associated with self-assembly peptide surfactants.

    Science.gov (United States)

    Zheng, Xuan; Dong, Shuangshuang; Zheng, Jie; Li, Duanhua; Li, Feng; Luo, Zhongli

    2014-01-01

    G-protein coupled receptors (GPCRs) are involved in regulating most of physiological actions and metabolism in the bodies, which have become most frequently addressed therapeutic targets for various disorders and diseases. Purified GPCR-based drug discoveries have become routine that approaches to structural study, novel biophysical and biochemical function analyses. However, several bottlenecks that GPCR-directed drugs need to conquer the problems including overexpression, solubilization, and purification as well as stabilization. The breakthroughs are to obtain efficient protein yield and stabilize their functional conformation which are both urgently requiring of effective protein synthesis system methods and optimal surfactants. Cell-free protein synthesis system is superior to the high yields and post-translation modifications, and early signs of self-assembly peptide detergents also emerged to superiority in purification of membrane proteins. We herein focus several predominant protein synthesis systems and surfactants involving the novel peptide detergents, and uncover the advantages of cell-free protein synthesis system with self-assembling peptide detergents in purification of functional GPCRs. This review is useful to further study in membrane proteins as well as the new drug exploration.

  16. Complement system part II: role in immunity

    Directory of Open Access Journals (Sweden)

    Nicolas S. Merle

    2015-05-01

    Full Text Available The complement system has been considered for a long time as a simple lytic system, aimed to kill bacteria infecting the host organism. Nowadays this vision has changed and it is well accepted that complement is a complex innate immune surveillance system, playing a key role in host homeostasis, inflammation and in the defense against pathogens. This review discusses recent advances in the understanding of the role of complement in physiology and pathology. It starts with a description of complement contribution to the normal physiology (homeostasis of a healthy organism, including the silent clearance of apoptotic cells and maintenance of cell survival. In pathology, complement can be a friend or a foe. It acts as a friend in the defense against pathogens, by inducing a direct killing by C5b-9 membrane attack complex by triggering inflammatory responses with the anaphylatoxins C3a and C5a and helps the mounting of an adaptive immune response, involving antigen presenting cells, T- and B- lymphocytes. But it can be also an enemy, when pathogens hijack complement regulators to protect themselves from the immune system. Also examples will be discussed, where inadequate complement activation becomes a disease cause, including atypical hemolytic uremic syndrome (aHUS, C3 glomerulopathies (C3G and systemic lupus erythematosus (SLE. Age related macular degeneration (AMD and cancer will be described as examples showing that complement contributes to a large variety of diseases, far exceeding the classical examples of diseases associated with complement deficiencies. Finally, we discuss complement as a therapeutic target.

  17. Automatic system for corneal ulcer diagnostic: II

    Science.gov (United States)

    Ventura, Liliane; Chiaradia, Caio; Faria de Sousa, Sidney J.

    1998-06-01

    Corneal Ulcer is a deepithelization of the cornea and it is a very common disease in agricultural countries. The clinician most used parameter in order to identify a favorable ulcer evolution is the regress of the affected area. However, this kind of evaluation is subjective, once just the horizontal and vertical axes are measured based on a graduated scale and the affected area is estimated. Also, the registration of the disease is made by photographs. In order to overcome the subjectiveness and to register the images in a more accessible way (hard disks, floppy disks, etc.), we have developed an automatic system in order to evaluate the affected area (the ulcer). An optical system is implemented in a Slit Lamp (SL) and connected to a CCD detector. The image is displayed in PC monitor by a commercial frame grabber and a dedicated software for determining the area of the ulcer (precision of 20 mm) has been developed.

  18. Sensitive detection of biothiols and histidine based on the recovered fluorescence of the carbon quantum dots–Hg(II) system

    Energy Technology Data Exchange (ETDEWEB)

    Hou, Juan; Zhang, Fengshuang; Yan, Xu; Wang, Long; Yan, Jin [College of Chemistry, Jilin University, 2699 Qianjin Street, Changchun 130012 (China); Ding, Hong [State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of Chemistry, Jilin University, Changchun 130012 (China); Ding, Lan, E-mail: dinglan@jlu.edu.cn [College of Chemistry, Jilin University, 2699 Qianjin Street, Changchun 130012 (China)

    2015-02-15

    Highlights: • Carbon quantum dots-based probe was used for detection of GSH, Cys or His. • The fluorescence of CQDs was quenched by Hg(II) and then recovered by GSH, Cys or His. • No further surface modification or purification of CQDs was required. • This sensor exhibits superior accuracy and sensitivity. • The proposed method was simple in design, fast in operation. - Abstract: In this paper, we presented a novel, rapid and highly sensitive sensor for glutathione (GSH), cysteine (Cys) and histidine (His) based on the recovered fluorescence of the carbon quantum dots (CQDs)–Hg(II) system. The CQDs were synthesized by microwave-assisted approach in one pot according to our previous report. The fluorescence of CQDs could be quenched in the presence of Hg(II) due to the coordination occurring between Hg(II) and functional groups on the surface of CQDs. Subsequently, the fluorescence of the CQDs–Hg(II) system was recovered gradually with the addition of GSH, Cys or His due to their stronger affinity with Hg(II). A good linear relationship was obtained from 0.10 to 20 μmol L{sup −1} for GSH, from 0.20 to 45 μmol L{sup −1} for Cys and from 0.50 to 60 μmol L{sup −1} for His, respectively. This method has been successfully applied to the trace detection of GSH, Cys or His in human serum samples with satisfactory results. The proposed method was simple in design and fast in operation, which demonstrated great potential in bio-sensing fields.

  19. Complement System Part II: Role in Immunity

    Science.gov (United States)

    Merle, Nicolas S.; Noe, Remi; Halbwachs-Mecarelli, Lise; Fremeaux-Bacchi, Veronique; Roumenina, Lubka T.

    2015-01-01

    The complement system has been considered for a long time as a simple lytic cascade, aimed to kill bacteria infecting the host organism. Nowadays, this vision has changed and it is well accepted that complement is a complex innate immune surveillance system, playing a key role in host homeostasis, inflammation, and in the defense against pathogens. This review discusses recent advances in the understanding of the role of complement in physiology and pathology. It starts with a description of complement contribution to the normal physiology (homeostasis) of a healthy organism, including the silent clearance of apoptotic cells and maintenance of cell survival. In pathology, complement can be a friend or a foe. It acts as a friend in the defense against pathogens, by inducing opsonization and a direct killing by C5b–9 membrane attack complex and by triggering inflammatory responses with the anaphylatoxins C3a and C5a. Opsonization plays also a major role in the mounting of an adaptive immune response, involving antigen presenting cells, T-, and B-lymphocytes. Nevertheless, it can be also an enemy, when pathogens hijack complement regulators to protect themselves from the immune system. Inadequate complement activation becomes a disease cause, as in atypical hemolytic uremic syndrome, C3 glomerulopathies, and systemic lupus erythematosus. Age-related macular degeneration and cancer will be described as examples showing that complement contributes to a large variety of conditions, far exceeding the classical examples of diseases associated with complement deficiencies. Finally, we discuss complement as a therapeutic target. PMID:26074922

  20. Purification and characterization of a thermostable glucoamylase ...

    African Journals Online (AJOL)

    STORAGESEVER

    2010-06-07

    Jun 7, 2010 ... tryptophan and serine residues in the catalytic process. Raw corn .... selected for amylase production, purification and characterization. .... chromatogram was developed with solvent system of butanol/ ... starch affinity and acetone precipitation method for ..... Optimization of extraction and purification of.

  1. Partition Efficiency of High-Pitch Locular Multilayer Coil for Countercurrent Chromatographic Separation of Proteins Using Small-Scale Cross-Axis Coil Planet Centrifuge and Application to Purification of Various Collagenases with Aqueous-Aqueous Polymer Phase Systems.

    Science.gov (United States)

    Shinomiya, Kazufusa; Kobayashi, Hiroko; Inokuchi, Norio; Nakagomi, Kazuya; Ito, Yoichiro

    2011-01-01

    Partition efficiency of the high-pitch locular multilayer coil was evaluated in countercurrent chromatographic (CCC) separation of proteins with an aqueous-aqueous polymer phase system using the small-scale cross-axis coil planet centrifuge (X-axis CPC) fabricated in our laboratory. The separation column was specially made by high-pitch (ca 5 cm) winding of 1.0 mm I.D., 2.0 mm O.D. locular tubing compressed at 2 cm intervals with a total capacity of 29.5 mL. The protein separation was performed using a set of stable proteins including cytochrome C, myoglobin, and lysozyme with the 12.5% (w/w) polyethylene glycol (PEG) 1000 and 12.5% (w/w) dibasic potassium phosphate system (pH 9.2) under 1000 rpm of column revolution. This high-pitch locular tubing yielded substantially increased stationary phase retention than the normal locular tubing for both lower and upper mobile phases. In order to demonstrate the capability of the high-pitch locular tubing, the purification of collagenase from the crude commercial sample was carried out using an aqueous-aqueous polymer phase system. Using the 16.0% (w/w) PEG 1000 - 6.3% (w/w) dibasic potassium phosphate - 6.3% (w/w) monobasic potassium phosphate system (pH 6.6), collagenase I, II, V and X derived from Clostridium hystolyticum were separated from other proteins and colored small molecular weight compounds present in the crude commercial sample, while collagenase N-2 and S-1 from Streptomyces parvulus subsp. citrinus were eluted with impurities at the solvent front with the upper phase. The collagenase from C. hystolyticum retained its enzymatic activity in the purified fractions. The overall results demonstrated that the high-pitch locular multilayer coil is effectively used for the CCC purification of bioactive compounds without loss of their enzymatic activities.

  2. Design and Implementation of an Embedded NIOS II System for JPEG2000 Tier II Encoding

    Directory of Open Access Journals (Sweden)

    John M. McNichols

    2013-01-01

    Full Text Available This paper presents a novel implementation of the JPEG2000 standard as a system on a chip (SoC. While most of the research in this field centers on acceleration of the EBCOT Tier I encoder, this work focuses on an embedded solution for EBCOT Tier II. Specifically, this paper proposes using an embedded softcore processor to perform Tier II processing as the back end of an encoding pipeline. The Altera NIOS II processor is chosen for the implementation and is coupled with existing embedded processing modules to realize a fully embedded JPEG2000 encoder. The design is synthesized on a Stratix IV FPGA and is shown to out perform other comparable SoC implementations by 39% in computation time.

  3. The Brookside Farm Wetland Ecosystem Treatment (WET System: A Low-Energy Methodology for Sewage Purification, Biomass Production (Yield, Flood Resilience and Biodiversity Enhancement

    Directory of Open Access Journals (Sweden)

    Julian C. Abrahams

    2017-01-01

    Full Text Available Wastewater from domestic developments, farms and agro-industrial processing can be sources of pollution in the environment; current wastewater management methods are usually machine-based, and thus energy consuming. When Permaculture Principles are used in the creation of water purification and harvesting systems, there can be multiple environmental and economic benefits. In the context of energy descent, it may be considered desirable to treat wastewater using minimal energy. The constructed wetland design presented here is a low-entropy system in which wastewater is harvested and transformed into lush and productive wetland, eliminating the requirement for non-renewable energy in water purification, and also maximising benefits: biodiversity, flood resilience and yield. In permaculture design, the high concentrations of nitrogen and phosphorous compounds in sewage are viewed as valuable nutrients, resources to be harvested by a constructed wetland ecosystem and converted into useful yield. Similarly, rainwater runoff is not viewed as a problem which can cause flooding, but as a potential resource to be harvested to provide a yield. This paper presents a case study, with both water quality and productivity data, from Brookside Farm UK, where the use of Permaculture Design Principles has created a combined wastewater management and purification system, accepting all site water.

  4. Experimental study of the portable blood purification system for treatment of acute renal failure in dogs under the field condition in a high-altitude environment

    Directory of Open Access Journals (Sweden)

    Hai-bin LI

    2014-01-01

    Full Text Available Objective To reproduce the model of acute renal failure (ARF in beagles for comprehensively evaluating the safety, stability and validity of the continuous blood purification system under the field condition in a high-altitude environment. Methods Adult beagle ARF model was reproduced by bilateral nephrectomy. All ARF dogs underwent continuous veno-venous hemofiltration with a blood purification machine. Vital signs, renal function, liver function and plasma ion-levels before and after the therapy were analyzed through self-controlled study. Performance of the continuous blood purification machine running in a highaltitude environment was evaluated. Results Blood urea nitrogen (BUN and creatinine (Cr of six dogs were assayed 24 hours after nephrectomy, the difference showed statistical significance compared with those before the surgery (P<0.05, implying that six ARF models were reproduced successfully. Six dogs experienced continuous renal replacement therapy (CRRT with external jugular vein double-lumen tube placement (3 dogs or femoral vein catheter placement (3 dogs. Five dogs successfully finished the whole experiment, and one dog died because of falling off of the dialysis tube and blood loss. The continuous blood purification machine was sensitive with rapid release of warning signals when the condition was abnormal. Compared to that before CRRT, there were no statistically significant differences in heart rate, respiratory rate and blood pressure in ARF models 2, 4 hours after CRRT (P>0.05. And there were no statistically significant differences in ALT, AST and T-Bil in ARF models between that before and 2, 4 hours after CRRT (P>0.05. However, 2, 4 hours after CRRT, BUN and Cr levels descended significantly (P<0. 05, so was K+ level (P<0. 05. There were no complications such as hemorrhage or hypotension. There were no statistical differences in volume of replacement fluid or discarded fluid between before and after CRRT

  5. Chemical looping integration with a carbon dioxide gas purification unit

    Energy Technology Data Exchange (ETDEWEB)

    Andrus, Jr., Herbert E.; Jukkola, Glen D.; Thibeault, Paul R.; Liljedahl, Gregory N.

    2017-01-24

    A chemical looping system that contains an oxidizer and a reducer is in fluid communication with a gas purification unit. The gas purification unit has at least one compressor, at least one dryer; and at least one distillation purification system; where the gas purification unit is operative to separate carbon dioxide from other contaminants present in the flue gas stream; and where the gas purification unit is operative to recycle the contaminants to the chemical looping system in the form of a vent gas that provides lift for reactants in the reducer.

  6. Bifunctional redox flow battery - 2. V(III)/V(II)-L-cystine(O{sub 2}) system

    Energy Technology Data Exchange (ETDEWEB)

    Wen, Y.H.; Xun, Y. [Research Institute of Chemical Defense, Beijing 100083 (China); Cheng, J.; Yang, Y.S. [Research Institute of Chemical Defense, Beijing 100083 (China); Beijing Science and Technology University, Beijing 100083 (China); Ma, P.H. [Full Cell R and D Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, Liaoning 116023 (China)

    2008-08-20

    A new bifunctional redox flow battery (BRFB) system, V(III)/V(II) - L-cystine(O{sub 2}), was systematically investigated by using different separators. It is shown that during charge, water transfer is significantly restricted with increasing the concentration of HBr when the Nafion 115 cation exchange membrane is employed. The same result can be obtained when the gas diffusion layer (GDL) hot-pressed separator is used. The organic electro-synthesis is directly correlated with the crossover of vanadium. When employing the anion exchange membrane, the electro-synthesis efficiency is over 96% due to a minimal crossover of vanadium. When the GDL hot-pressed separator is applied, the crossover of vanadium and water transfer are noticeably prevented and the electro-synthesis efficiency of over 99% is obtained. Those impurities such as vanadium ions and bromine can be eliminated through the purification of organic electro-synthesized products. The purified product is identified to be L-cysteic acid by IR spectrum. The BRFB shows a favorable discharge performance at a current density of 20 mA cm{sup -2}. Best discharge performance is achieved by using the GDL hot-pressed separator. The coulombic efficiency of 87% and energy efficiency of about 58% can be obtained. The cause of major energy losses is mainly associated with the cross-contamination of anodic and cathodic active electrolytes. (author)

  7. Optimization of Serine Protease Purification from Mango (Mangifera indica cv. Chokanan Peel in Polyethylene Glycol/Dextran Aqueous Two Phase System

    Directory of Open Access Journals (Sweden)

    Abdul Manap Mohd Yazid

    2012-03-01

    Full Text Available Mango peel is a good source of protease but remains an industrial waste. This study focuses on the optimization of polyethylene glycol (PEG/dextran-based aqueous two-phase system (ATPS to purify serine protease from mango peel. The activity of serine protease in different phase systems was studied and then the possible relationship between the purification variables, namely polyethylene glycol molecular weight (PEG, 4000–12,000 g·mol−1, tie line length (−3.42–35.27%, NaCl (−2.5–11.5% and pH (4.5–10.5 on the enzymatic properties of purified enzyme was investigated. The most significant effect of PEG was on the efficiency of serine protease purification. Also, there was a significant increase in the partition coefficient with the addition of 4.5% of NaCl to the system. This could be due to the high hydrophobicity of serine protease compared to protein contaminates. The optimum conditions to achieve high partition coefficient (84.2 purification factor (14.37 and yield (97.3% of serine protease were obtained in the presence of 8000 g·mol−1 of PEG, 17.2% of tie line length and 4.5% of NaCl at pH 7.5. The enzymatic properties of purified serine protease using PEG/dextran ATPS showed that the enzyme could be purified at a high purification factor and yield with easy scale-up and fast processing.

  8. Design of aqueous two-phase systems for purification of hyaluronic acid produced by metabolically engineered Lactococcus lactis.

    Science.gov (United States)

    Rajendran, Vivek; Puvendran, Kirubhakaran; Guru, Bharath Raja; Jayaraman, Guhan

    2016-02-01

    Hyaluronic acid has a wide range of biomedical applications and its commercial value is highly dependent on its purity and molecular weight. This study highlights the utility of aqueous two-phase separation as a primary recovery step for hyaluronic acid and for removal of major protein impurities from fermentation broths. Metabolically engineered cultures of a lactate dehydrogenase mutant strain of Lactococcus lactis (L. lactis NZ9020) were used to produce high-molecular-weight hyaluronic acid. The cell-free fermentation broth was partially purified using a polyethylene glycol/potassium phosphate system, resulting in nearly 100% recovery of hyaluronic acid in the salt-rich bottom phase in all the aqueous two-phase separation experiments. These experiments were optimized for maximum removal of protein impurities in the polyethylene glycol rich top phase. The removal of protein impurities resulted in substantial reduction of membrane fouling in the subsequent diafiltration process, carried out with a 300 kDa polyether sulfone membrane. This step resulted in considerable purification of hyaluronic acid, without any loss in recovery and molecular weight. Diafiltration was followed by an adsorption step to remove minor impurities and achieve nearly 100% purity. The final hyaluronic acid product was characterized by Fourier-transform IR and NMR spectroscopy, confirming its purity.

  9. A Hydroponic System for Purification of Anaerobically Treated Dairy Manure and Production of Wheat as a Nutritional Forage Crop

    Directory of Open Access Journals (Sweden)

    Abdel E. Ghaly

    2007-01-01

    Full Text Available A hydroponic system was developed and used for purification of an anaerobically treated dairy manure and production of forage crops. The effect of light duration, seeding rate and wastewater application rate on the crop yield and pollution potential reduction were studied. The results indicated that a wheat forage crop can be produced in 21 days from germination to harvest in this system and removal efficiencies of up to 89.9, 94.6, and 86.7 % can be achieved for the total solids, chemical oxygen demand (COD and ammonium nitrogen, respectively. Increasing the wastewater application rate increased the crop yield and decreased the pollutants removal efficiencies. A treatment combination of wastewater application rate of 900 mL/day, a seeding rate of 400 g and a light duration of 12 hours gave the best results for crop yield (3.65 kg of wheat/tray. A total possible yield of 3160 tonnes per hectare per year can be achieved with the system (with thirteen harvests per year. This is more than 98 times greater than the yield obtainable from a field grown conventional forage of 245 tonnes per hectare per year. At the optimum forage production, removal efficiencies of 75.7, 85.9 and 75.6% were achieved for the solids, COD, ammonium nitrogen, respectively. A nitrate nitrogen concentration of 6.7 mg/L was found in the effluent from the hydroponic system. This is below the Canadian Environmental and Health Guidelines of 10 mg/L.

  10. Sensory systems II senses other than vision

    CERN Document Server

    Wolfe, Jeremy M

    1988-01-01

    This series of books, "Readings from the Encyclopedia of Neuroscience." consists of collections of subject-clustered articles taken from the Encyclopedia of Neuroscience. The Encyclopedia of Neuroscience is a reference source and compendium of more than 700 articles written by world authorities and covering all of neuroscience. We define neuroscience broadly as including all those fields that have as a primary goal the under­ standing of how the brain and nervous system work to mediate/control behavior, including the mental behavior of humans. Those interested in specific aspects of the neurosciences, particular subject areas or specialties, can of course browse through the alphabetically arranged articles of the En­ cyclopedia or use its index to find the topics they wish to read. However. for those readers-students, specialists, or others-who will find it useful to have collections of subject-clustered articles from the Encyclopedia, we issue this series of "Readings" in paperback. Students in neuroscienc...

  11. Theoretical analysis of BLM system for HLS II

    CERN Document Server

    Yukai, Chen; Lijuan, He; Weimin, Li

    2014-01-01

    Hefei Light Source (HLS) is being upgraded to HLS II. Its emittance will be much lower than before, therefore the Touschek scattering will increase significantly and become the dominant factor of beam loss. So it is necessary to build a new beam loss monitoring (BLM) system differed from the old one to obtain the quantity and position information of lost electrons. This information is useful in the commissioning, troubleshooting and beam lifetime studying for HLS II. This paper analyzes the distribution features of different kinds of lost electrons, introduces the new machine's operation parameters and discusses the way to choose proper monitoring positions. Base on these comprehensive analysis, a new BLM system for HLS II is proposed.

  12. Characterization of optical systems for the ALPS II experiment

    Science.gov (United States)

    Spector, Aaron D.; Põld, Jan H.; Bähre, Robin; Lindner, Axel; Willke, Benno

    2016-12-01

    ALPS II is a light shining through a wall style experiment that will use the principle of resonant enhancement to boost the conversion and reconversion probabilities of photons to relativistic WISPs. This will require the use of long baseline low-loss optical cavities. Very high power build up factors in the cavities must be achieved in order to reach the design sensitivity of ALPS II. This necessitates a number of different sophisticated optical and control systems to maintain the resonance and ensure maximal coupling between the laser and the cavity. In this paper we report on the results of the characterization of these optical systems with a 20 m cavity and discuss the results in the context of ALPS II.

  13. Theoretical analysis of BLM system for HLS II

    Science.gov (United States)

    Chen, Yu-Kai; Li, Yu-Xiong; Li, Wei-Min; He, Li-Juan

    2015-01-01

    Hefei Light Source (HLS) is being upgraded to HLS II. Its emittance will be much lower than before, therefore the Touschek scattering will increase significantly and become the dominant factor of beam loss. So it is necessary to build a new beam loss monitoring (BLM) system that, in contrast to the old one, is able to obtain the quantity and position information of lost electrons. This information is useful in the commissioning, troubleshooting, and beam lifetime studying for HLS II. This paper analyzes the distribution features of different kinds of lost electrons, introduces the operation parameters of the new machine and discusses how to choose proper monitoring positions. Based on these comprehensive analyses, a new BLM system for HLS II is proposed.

  14. Characterization of optical systems for the ALPS II experiment

    Energy Technology Data Exchange (ETDEWEB)

    Spector, Aaron D. [Hamburg Univ. (Germany). Inst. fuer Experimentalphsik; Pold, Jan H.; Lindner, Axel [Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); Baehre, Robin; Willke, Benno [Max-Planck-Institute for Gravitational Physics, Hannover (Germany); Hannover Univ. (Germany). Inst. fuer Gravitationsphysik

    2016-09-15

    ALPS II is a light shining through a wall style experiment that will use the principle of resonant enhancement to boost the conversion and reconversion probabilities of photons to relativistic WISPs. This will require the use of long baseline low-loss optical cavities. Very high power build up factors in the cavities must be achieved in order to reach the design sensitivity of ALPS II. This necessitates a number of different sophisticated optical and control systems to maintain the resonance and ensure maximal coupling between the laser and the cavity. In this paper we report on the results of the characterization of these optical systems with a 20m cavity and discuss the results in the context of ALPS II.

  15. Characterization of optical systems for the ALPS II experiment

    CERN Document Server

    Spector, Aaron D; Bähre, Robin; Lindner, Axel; Willke, Benno

    2016-01-01

    ALPS II is a light shining through a wall style experiment that will use the principle of resonant enhancement to boost the conversion and reconversion probabilities of photons to relativistic WISPs. This will require the use of long baseline low-loss optical cavities. Very high power build up factors in the cavities must be achieved in order to reach the design sensitivity of ALPS II. This necessitates a number of different sophisticated optical and control systems to maintain the resonance and ensure maximal coupling between the laser and the cavity. In this paper we report on the results of the characterization of these optical systems with a 20 m cavity and discuss the results in the context of ALPS II.

  16. System Aero-Accelator for the purification of biodegradable effluents; Sistema aero-accelator para la depuracion de efluentes biodegradables (I)

    Energy Technology Data Exchange (ETDEWEB)

    Bosque Hernandez, J. L. del; Martin Sanchez, J. L. [Universidad de Salamanca (Spain)

    2000-07-01

    The contamination of the waters is one of the factors that contributes to the deterioration of our environment and since it is a very limited one its treatment descontaminant it is one of the politic's main objectives and environmental administration at all the levels, being spread to the total purification of the generated residual effluents. To reach this objective, big technological efforts are required that allow next to the creation of new processes, the adaptation of the processes existent depuratives, increasing the effectiveness of the same ones. One of the techniques of purification of possible recovery is the Compact System of active mires Aero-Accelator. Presently work is designed and it builds a plant pilot with Aero-Accelator geometry to study its behavior in the treatment of effluents of urban type with different loads pollutants. (Author) 16 refs.

  17. Upgrade of Beam Energy Measurement System at BEPC-II

    CERN Document Server

    Zhang, Jian-Yong; Mo, Xiao-Hu; Guo, Di-Zhou; Wang, Jian-Li; Liu, Bai-Qi; Achasov, M N; Krasnov, A A; Muchnoi, N Yu; Pyata, E E; Mamoshkina, E V; Harris, F A

    2015-01-01

    The beam energy measurement system is of great importance and profit for both BEPC-II accelerator and BES-III detector. The system is based on measuring the energies of Compton back-scattered photons. Many advanced techniques and precise instruments are employed to realize the highly accurate measurement of positron/electron beam energy. During five year's running period, in order to meet the requirement of data taking and improve the capacity of measurement itself, the upgradation of system is continued, which involve the component reformation of laser and optics subsystem, replacement of view-port of the laser to vacuum insertion subsystem, the purchase of electric cooling system for high purity germanium detector, and the improvement of data acquisition and processing subsystem. The upgrading of system guarantees the smooth and efficient measuring of beam energy at BEPC-II and accommodates the accurate offline energy values for further physics analysis at BES-III.

  18. 天然气净化厂火炬系统设计研究%Design of the Flare System of Natural Gas Purification Plants

    Institute of Scientific and Technical Information of China (English)

    孙一伦

    2014-01-01

    The flare system design of natural gas purification plants is different from ordinary refineries. The flare gas in natural gas purification plants has high sulfur content and low temperature. And current purification plants constructed by Sinopec Group require device maintenance in batches, namely the flare system can not shut down, which will propose new design issues and requirements.%天然气净化厂火炬系统的设计与普通炼油厂火炬系统设计有所不同。天然气净化厂中的放空火炬气含硫量高、放空温度低。而且目前中石化所建设的净化厂项目均要求装置轮番检修,即火炬系统不能停工,对设计提出了新的课题和要求。

  19. A novel aqueous micellar two-phase system composed of surfactant and sorbitol for purification of pectinase enzyme from Psidium guajava and recycling phase components.

    Science.gov (United States)

    Amid, Mehrnoush; Murshid, Fara Syazana; Manap, Mohd Yazid; Hussin, Muhaini

    2015-01-01

    A novel aqueous two-phase system composed of a surfactant and sorbitol was employed for the first time to purify pectinase from Psidium guajava. The influences of different parameters, including the type and concentration of the surfactant and the concentration and composition of the surfactant/sorbitol ratio, on the partitioning behavior and recovery of pectinase were investigated. Moreover, the effects of system pH and the crude load on purification fold and the yield of purified pectinase were studied. The experimental results indicated that the pectinase was partitioned into surfactant-rich top phase, and the impurities were partitioned into the sorbitol-rich bottom phase with the novel method involving an ATPS composed of 26% (w/w) Triton X-100 and 23% (w/w) sorbitol at 54.2% of the TLL crude load of 20% (w/w) at pH 6.0. The enzyme was successfully recovered by this method with a high purification factor of 15.2 and a yield of 98.3%, whereas the phase components were also recovered and recycled at rates above 96%. This study demonstrated that this novel ATPS method can be used as an efficient and economical alternative to the traditional ATPS for the purification and recovery of the valuable enzyme.

  20. Kilowatt isotope power system phase II plan. Volume II: flight System Conceptual Design (FSCD)

    Energy Technology Data Exchange (ETDEWEB)

    1978-03-01

    The Kilowatt Isotope Power System (KIPS) Flight System Conceptual Design (FSCD) is described. Included are a background, a description of the flight system conceptual design, configuration of components, flight system performance, Ground Demonstration System test results, and advanced development tests.

  1. 78 FR 71785 - Passenger Train Emergency Systems II

    Science.gov (United States)

    2013-11-29

    ... From the Federal Register Online via the Government Publishing Office ] Vol. 78 Friday, No. 230 November 29, 2013 Part III Department of Transportation Federal Railroad Administration 49 CFR Parts 238 and 239 Passenger Train Emergency Systems II; Final Rule #0;#0;Federal Register / Vol. 78 , No....

  2. Performance assessment of the SOFA, APACHE II scoring system, and SAPS II in intensive care unit organophosphate poisoned patients.

    Science.gov (United States)

    Kim, Yong Hwan; Yeo, Jung Hoon; Kang, Mun Ju; Lee, Jun Ho; Cho, Kwang Won; Hwang, SeongYoun; Hong, Chong Kun; Lee, Young Hwan; Kim, Yang Weon

    2013-12-01

    This study assessed the ability of the Sequential Organ Failure Assessment (SOFA) and Acute Physiology, Chronic Health Evaluation (APACHE) II scoring systems, as well as the Simplified Acute Physiology Score (SAPS) II method to predict group mortality in intensive care unit (ICU) patients who were poisoned with organophosphate. The medical records of 149 organophosphate poisoned patients admitted to the ICU from September 2006 to December 2012 were retrospectively examined. The SOFA, APACHE II, and SAPS II were calculated based on initial laboratory data in the Emergency Department, and during the first 24 hr of ICU admission. The probability of death was calculated for each patient based on the SOFA score, APACHE II score, and SAPS II equations. The ability to predict group mortality by the SOFA score, APACHE II score, and SAPS II method was assessed using two by two decision matrices and receiver operating characteristic (ROC) curve analysis. A total of 131 patients (mean age, 61 yr) were enrolled. The sensitivities, specificities, and accuracies were 86.2%, 82.4%, and 83.2% for the SOFA score, respectively; 65.5%, 68.6%, and 67.9% for the APACHE II scoring system, respectively; and 86.2%, 77.5%, and 79.4% for the SAPS II, respectively. The areas under the curve in the ROC curve analysis for the SOFA score, APACHE II scoring system, and SAPS II were 0.896, 0.716, and 0.852, respectively. In conclusion, the SOFA, APACHE II, and SAPS II have different capability to discriminate and estimate early in-hospital mortality of organophosphate poisoned patients. The SOFA score is more useful in predicting mortality, and easier and simpler than the APACHE II and SAPS II.

  3. [Isolation and purification of alpha-glycerophosphate oxidase in a polyethylene glycol/(NH4 )2SO4 aqueous two-phase system].

    Science.gov (United States)

    Meng, Yao; Jin, Jiagui; Liu, Shuangfeng; Yang, Min; Zhang, Qinglian; Wan, Li; Tang, Kun

    2014-02-01

    Alpha-glycerophosphate oxidase (alpha-GPO) from Enterococcus casseli flavus was successfully isolated and purified by using polyethylene glycol (PEG)/(NH4)2SO4 aqueous two-phase system (ATPS). The results showed that the chosen PEG/(NH4)2SO4 ATPS could be affected by PEG molecular weight, pH, concentration of PEG and (NH4)2SO4, and inorganic salt as well as additional amount of crude enzyme. After evaluating these influencing factors, the final optimum purification strategy was formed by 16.5% (m/m) PEG2000, 13.2% (m/m) (NH4)2SO4, pH 7.5 and 30% (m/m) additive crude enzyme, respectively. The NaCl was a negative influencing factor which would lead to lower purification fold and activity recovery. These conditions eventually resulted in the activity recovery of 89% (m/m), distribution coefficient of 1.2 and purification fold of 7.0.

  4. Development of a chamber system for rapid, high yield and cost-effective purification of deoxyribonucleic acid fragments from agarose gel

    Directory of Open Access Journals (Sweden)

    Gilda Eslami

    2014-01-01

    Full Text Available Background: There are several methods commonly practicing for deoxyribonucleic acid (DNA purification from agarose gel. In most laboratories, especially in developing countries, present methods for recovering of DNA fragments from the gel are mostly involved organic solvents. However, manual purification using organic solvents are toxic, labor intensive, time consuming and prone to contamination owing to several handling steps. The above mentioned burdens as well as cost and long time to import them, especially in developing countries, prompted us to design and develop a chamber system for rapid, non-toxic, cost-effective and user friendly device for polymerase chain reaction (PCR products purification from agarose gel. Materials and Methods: The device was made from plexiglass plates. After amplification of two fragments of 250 and 850 bp, PCR products were electrophoresed. Subsequently, the desired bands were excised and purified with three method: HiPer Mini chamber, phenol extraction method and spin column procedure. To assess the suitability of the purified DNAs, restriction digestion was applied. Results: Results showed that the yield of recovered DNA in our method was above 95%, whereas the yields obtained with conventional phenol extraction and spin column methods were around 60%. Conclusion: In conclusion, the current method for DNA elution is quick, inexpensive and robust and it does not require the use of toxic organic solvents. In addition, the purified DNA was well has suited for further manipulations such as restriction digestion, ligation, cloning, sequencing and hybridization.

  5. A new and convenient method for purification of {sup 86}Y using a Sr(II) selective resin and comparison of biodistribution of {sup 86}Y and {sup 111}In labeled Herceptin{sup TM}

    Energy Technology Data Exchange (ETDEWEB)

    Garmestani, Kayhan; Milenic, Diane E.; Plascjak, Paul S.; Brechbiel, Martin W. E-mail: martinwb@mail.nih.gov

    2002-07-01

    A simple and rapid procedure was developed for purification of cyclotron produced {sup 86}Y via the {sup 86}Sr(p,n) {sup 86}Y reaction. A commercially available Sr(II) selective resin was used to separate {sup 86}Y from the cyclotron irradiated Sr(II) target with a recovery of the enriched Sr(II) target while yielding a 75-80% recovery of {sup 86}Y suitable for radiolabeling either proteins or peptides. To demonstrate the utility of this methodology, the anti-HER2 monoclonal antibody Herceptin{sup TM} was radiolabeled with the purified {sup 86}Y and compared to {sup 111}In labeled Herceptin{sup TM}. The biodistribution study demonstrated that {sup 111}In-Herceptin{sup TM}, while a suitable surrogate for {sup 90}Y in the major organs, did not parallel the uptake of {sup 86}Y-Herceptin{sup TM} in the bone, and thus may not accurately predict the level of {sup 90}Y accumulation in the bone for clinical RIT applications. This result exemplifies the requirement of employing appropriate matched pair isotopes for imaging and therapy to insure that dosimetry considerations may be addressed accurately.

  6. Air/Water Purification

    Science.gov (United States)

    1992-01-01

    After 18 years of research into air/water pollution at Stennis Space Center, Dr. B. C. Wolverton formed his own company, Wolverton Environmental Services, Inc., to provide technology and consultation in air and water treatment. Common houseplants are used to absorb potentially harmful materials from bathrooms and kitchens. The plants are fertilized, air is purified, and wastewater is converted to clean water. More than 100 U.S. communities have adopted Wolverton's earlier water hyacinth and artificial marsh applications. Catfish farmers are currently evaluating the artificial marsh technology as a purification system.

  7. Water Purification Product

    Science.gov (United States)

    2004-01-01

    Ecomaster, an affiliate of BioServe Space Technologies, this PentaPure technology has been used to purify water for our nation's Space Shuttle missions since 1981. WTC-Ecomaster of Mirneapolis, Minnesota manufactures water purification systems under the brand name PentaPure (TM). BioServe researcher Dr. George Marchin, of Kansas State University, first demonstrated the superiority of this technology and licensed it to WTC. Marchin continues to perform microgravity research in the development of new technologies for the benefit of life on Earth.

  8. Doppler reflectometer system in the stellarator TJ-II

    Energy Technology Data Exchange (ETDEWEB)

    Happel, T.; Estrada, T.; Blanco, E.; Tribaldos, V.; Cappa, A.; Bustos, A. [Laboratorio Nacional de Fusion, Asociacion Euratom-CIEMAT, 28040 Madrid (Spain)

    2009-07-15

    A Doppler reflectometer system has recently been installed in the stellarator TJ-II. The system is optimized for the Q-band (33-50 GHz) and the high-curvature plasmas produced in TJ-II. The launch angle of the microwave beam can be controlled by a steerable mirror to obtain angles between {+-}20 deg. enabling the measurement of perpendicular wave numbers in the range of 3-15 cm{sup -1}. The available angular range allows for comparisons between positive and negative values and additionally for calibration of the system. Localization and k{sub perpendicular}-estimation is done via the three-dimensional ray/beam-tracing code TRUBA. First measured spectra and radial profiles of the perpendicular velocity of plasma density fluctuations are presented.

  9. Ionic liquids for two-phase systems and their application for purification, extraction and biocatalysis.

    Science.gov (United States)

    Oppermann, Sebastian; Stein, Florian; Kragl, Udo

    2011-02-01

    The development of biotechnological processes using novel two-phase systems based on molten salts known as ionic liquids (ILs) got into the focus of interest. Many new approaches for the beneficial application of the interesting solvent have been published over the last years. ILs bring beneficial properties compared to organic solvents like nonflammability and nonvolatility. There are two possible ways to use the ILs: first, the hydrophobic ones as a substitute for organic solvents in pure two-phase systems with water and second, the hydrophilic ones in aqueous two-phase systems (ATPS). To effectively utilise IL-based two-phase systems or IL-based ATPS in biotechnology, extensive experimental work is required to gain the optimal system parameters to ensure selective extraction of the product of interest. This review will focus on the most actual findings dealing with the basic driving forces for the target extraction in IL-based ATPS as well as presenting some selected examples for the beneficial application of ILs as a substitute for organic solvents. Besides the research focusing on IL-based two-phase systems, the "green aspect" of ILs, due to their negligible vapour pressure, is widely discussed. We will present the newest results concerning ecotoxicity of ILs to get an overview of the state of the art concerning ILs and their utilisation in novel two-phase systems in biotechnology.

  10. Effect of IDA and TREN chelating agents and buffer systems on the purification of human IgG with immobilized nickel affinity membranes.

    Science.gov (United States)

    Ribeiro, Mariana Borsoi; Vijayalakshmi, Mookambesvaran; Todorova-Balvay, Daniele; Bueno, Sonia Maria Alves

    2008-01-01

    The purification of IgG from human plasma was studied by comparing two affinity membranes complexed with Ni(II), prepared by coupling iminodiacetic acid (IDA) and Tris(2-aminoethyl)amine (TREN) to poly(ethylenevinyl alcohol), PEVA, hollow fiber membranes. The Ni(II)-TREN-PEVA hollow fiber membrane had lower capacity for human IgG than the complex Ni(II)-IDA-PEVA, but with similar selectivity. The IgG in peak fractions eluted from the Ni(II)-IDA-PEVA with a stepwise concentration gradient of Tris-HCl pH 7.0 (100-700 mM) reached a purity of 98% (based on IgG, IgM, IgA, albumin, and transferrin nephelometric analysis). Adsorption IgG data at different temperatures (4-37 degrees C) were analyzed using Langmuir model resulting in a calculated maximum capacity at 25 degrees C of 204.6 mg of IgG/g of dry membrane. Decrease in Kd with increasing temperature (1.7x10(-5) to 5.3x10(-6) M) indicated an increase in affinity with increased temperature. The positive value of enthalpy change (26.2 kJ/mol) indicated that the adsorption of IgG in affinity membrane is endothermic. Therefore, lower temperature induces adsorption as verified experimentally.

  11. Lithium Propellant Purification and Filtration System For LFA and MPD Thrusters Project

    Data.gov (United States)

    National Aeronautics and Space Administration — Lithium has been proposed as an attractive metal propellant for advanced nuclear-electric propulsion missions in the outer solar system. While it is low molecular...

  12. Time, Temperature and Amount of Distilled Water Effects on the Purity and Yield of Bis(2-hydroxyethyl Terephthalate Purification System

    Directory of Open Access Journals (Sweden)

    H.W. Goh

    2015-07-01

    Full Text Available Polyethylene terephthalate (PET bottle is one of the common plastic wastes existed in the municipal solid waste in Malaysia. One alternative to solve the abundant of PET wastes is chemical recycling of the wastes to produce a value added product. This technology not only can decrease the PET wastes in landfill sites but also can produce many useful recycled PET products. Bis(2-hydroxyethyl terephthalate (BHET obtained from glycolysis reaction of PET waste was purified using crystallization process. The hot distilled water was added to glycolysis product followed by cooling and filtration to extract BHET in white solid form from the product. The effect of three operating conditions namely crystallization time, crystallization temperatures and amount of distilled water used to the yield of crystallization process were investigated. The purity of crystallization products were analyzed using HPLC and DSC. The optimum conditions of 3 hours crystallization time, 2 °C crystallization temperature and 5:1 mass ratio of distilled water used to glycolize solid gave the highest yield and purity of the crystallization process. © 2015 BCREC UNDIP. All rights reservedReceived: 12nd August 2014; Revised: 4th February 2015; Accepted: 5th February 2015How to Cite: Goh, H.W., Salmiaton, A., Abdullah, N., Idris, A. (2015. Time, Temperature and Amount of Distilled Water Effects on the Purity and Yield of Bis(2-hydroxyethyl Terephthalate Purification System. Bulletin of Chemical Reaction Engineering & Catalysis, 10 (2: 143-154. (doi:10.9767/bcrec.10.2.7195.143-154 Permalink/DOI: http://dx.doi.org/10.9767/bcrec.10.2.7195.143-154  

  13. A Novel Aqueous Two Phase System Composed of Surfactant and Xylitol for the Purification of Lipase from Pumpkin (Cucurbita moschata) Seeds and Recycling of Phase Components.

    Science.gov (United States)

    Amid, Mehrnoush; Manap, Mohd Yazid; Hussin, Muhaini; Mustafa, Shuhaimi

    2015-06-17

    Lipase is one of the more important enzymes used in various industries such as the food, detergent, pharmaceutical, textile, and pulp and paper sectors. A novel aqueous two-phase system composed of surfactant and xylitol was employed for the first time to purify lipase from Cucurbita moschata. The influence of different parameters such as type and concentration of surfactants, and the composition of the surfactant/xylitol mixtures on the partitioning behavior and recovery of lipase was investigated. Moreover, the effect of system pH and crude load on the degree of purification and yield of the purified lipase were studied. The results indicated that the lipase was partitioned into the top surfactant rich phase while the impurities partitioned into the bottom xylitol-rich phase using an aqueous two phase system composed of 24% (w/w) Triton X-100 and 20% (w/w) xylitol, at 56.2% of tie line length (TLL), (TTL is one of the important parameters in this study and it is determined from a bimodal curve in which the tie-line connects two nodes on the bimodal, that represent concentration of phase components in the top and bottom phases) and a crude load of 25% (w/w) at pH 8.0. Recovery and recycling of components was also measured in each successive step process. The enzyme was successfully recovered by the proposed method with a high purification factor of 16.4 and yield of 97.4% while over 97% of the phase components were also recovered and recycled. This study demonstrated that the proposed novel aqueous two phase system method is more efficient and economical than the traditional aqueous two phase system method for the purification and recovery of the valuable enzyme lipase.

  14. A Novel Aqueous Two Phase System Composed of Surfactant and Xylitol for the Purification of Lipase from Pumpkin (Cucurbita moschata Seeds and Recycling of Phase Components

    Directory of Open Access Journals (Sweden)

    Mehrnoush Amid

    2015-06-01

    Full Text Available Lipase is one of the more important enzymes used in various industries such as the food, detergent, pharmaceutical, textile, and pulp and paper sectors. A novel aqueous two-phase system composed of surfactant and xylitol was employed for the first time to purify lipase from Cucurbita moschata. The influence of different parameters such as type and concentration of surfactants, and the composition of the surfactant/xylitol mixtures on the partitioning behavior and recovery of lipase was investigated. Moreover, the effect of system pH and crude load on the degree of purification and yield of the purified lipase were studied. The results indicated that the lipase was partitioned into the top surfactant rich phase while the impurities partitioned into the bottom xylitol-rich phase using an aqueous two phase system composed of 24% (w/w Triton X-100 and 20% (w/w xylitol, at 56.2% of tie line length (TLL, (TTL is one of the important parameters in this study and it is determined from a bimodal curve in which the tie-line connects two nodes on the bimodal, that represent concentration of phase components in the top and bottom phases and a crude load of 25% (w/w at pH 8.0. Recovery and recycling of components was also measured in each successive step process. The enzyme was successfully recovered by the proposed method with a high purification factor of 16.4 and yield of 97.4% while over 97% of the phase components were also recovered and recycled. This study demonstrated that the proposed novel aqueous two phase system method is more efficient and economical than the traditional aqueous two phase system method for the purification and recovery of the valuable enzyme lipase.

  15. Technical and economic aspects of purification strategies to minimise discharge water from companies with closed soilless cultivation systems

    NARCIS (Netherlands)

    Os, E.A. van; Bruins, M.; Beerling, E.; Jurgens, R.; Appelman, W.; Enthoven, N.

    2014-01-01

    The aim of the research project was to achieve closure by two complementary means: 1) maximising reuse of the nutrient solution by solving problems in recirculation that leads to discharge, and 2) purification of the left over discharged water. In this paper the technical and economic aspects of pur

  16. Technical and economic aspects of purification strategies to minimise discharge water from companies with closed soilless cultivation systems

    NARCIS (Netherlands)

    Os, van E.A.; Bruins, M.A.; Beerling, E.A.M.; Jurgens, R.; Appelman, W.; Enthoven, N.

    2014-01-01

    The aim of the research project was to achieve closure by two complementary means: 1) maximising reuse of the nutrient solution by solving problems in recircula-tion that leads to discharge, and 2) purification of the left over discharged water. In this paper the technical and economic aspects of

  17. Assessment of internal contamination problems associated with bioregenerative air/water purification systems

    Science.gov (United States)

    Johnson, Anne H.; Bounds, B. Keith; Gardner, Warren

    1990-01-01

    The emphasis is to characterize the mechanisms of bioregenerative revitalization of air and water as well as to assess the possible risks associated with such a system in a closed environment. Marsh and aquatic plants are utilized for purposes of wastewater treatment as well as possible desalinization and demineralization. Foliage plants are also being screened for their ability to remove toxic organics from ambient air. Preliminary test results indicate that treated wastewater is typically of potable quality with numbers of pathogens such as Salmonella and Shigella significantly reduced by the artificial marsh system. Microbiological analyses of ambient air indicate the presence of bacilli as well as thermophilic actinomycetes.

  18. Standardization of water purification in the central dialysis fluid delivery system: validation and parametric method.

    Science.gov (United States)

    Tomo, Tadashi; Shinoda, Tosiho

    2009-01-01

    The central dialysis fluid delivery system (CDDS) has been mainly used for hemodialysis therapy in Japan. Validation and a parametric method are necessary for the quality control of dialysis fluid in CDDS. Validation is a concept for the assurance of system compatibility and product quality, and is defined as follows: the manufacturing and quality control methods including the system design and equipment of the manufacturing facility, manufacturing procedure and processes. Confirmed results must be kept within acceptable limits and they must be documented in a record. Important parameters for validating CDDS include: (1) setting the sterilized area; (2) decision of sterilization level; (3) confirmation of the maximum bio-burden; (4) performance of endotoxin retentive filter and reverse osmosis (RO) module, and (5) checkpoints of purity of dialysis water in the system. Taking the concept of validation and a parametric method in the management of CDDS into consideration enables the supply the purified dialysis fluid or the online prepared substitution fluid that meet the 2008 standards of the Japanese Society for Dialysis Therapy.

  19. From microsystems technology to the Saenger II space transportation system

    Science.gov (United States)

    Vogels, Hanns Arnt

    The role of space projects as drivers and catalysts of technology advances is discussed and illustrated from the perspective of the West German aerospace industry, summarizing a talk presented at the 1986 meeting of the German aerospace society DGLR. The history of space-transportation-system (STS) technology since the 1950s is traced, emphasizing the needs for greater payload weights and lower costs, and the design concept of Saenger II, a proposed two-stage ESA STS employing a hypersonic jet transport aircraft as its first stage, is outlined. It is argued that experience gained in developing the rocket-launched Hermes STS will be applicable to the second stage of Saenger II. Recent developments in microsystems (combining microelectronics, micromechanics, and microoptics), advanced materials (fiber-reinforced plastics, metals, and ceramics), and energy technology (hydrogen-based systems and solar cells) are surveyed, and their applicability to STSs is considered.

  20. [Potential of nitrification and denitrification in water purification system with hydroponic bio-filter method].

    Science.gov (United States)

    Li, Xian-ing; Lu, Xi-wu; Song, Hai-liang; Osamu, Nishimura; Yuhei, Inamori

    2005-03-01

    The potential of nitrification and denitrification of sediment and the density of ammonium-oxidizing bacteria and nitrite-oxidizing bacteria in sediment in water quality purifying system with hydroponic bio-filter method (HBFM) were measured. The variation of nitrification and denitrification potential of the sediment along the stream way was quantitatively studied. The results show that among the sediments from front, middle and retral part of the stream way, the sediment from middle part reached a maximum nitrification potential . nitrification potential of 4.76 x 10(-6) g/(g x h), while the sediment from front part reached a maximum denitrification potential of 8 .1 x 10(-7) g/(g x h). The distribution of nitrification potential accords with the ammonium-oxidizing bacteria density. The key for improving nitrogen removal efficiency of HBFM system consists in changing nitrification & denitrification region distributing and accordingly enhances denitrification process.

  1. Use of Barley for the Purification of Aquaculture Wastewater in a Hydroponics System

    Directory of Open Access Journals (Sweden)

    A. M. Snow

    2008-01-01

    Full Text Available Barley was examined for its ability to remove nutrients from aquaculture wastewater. The effects of seed sterilization using ethanol and bleach and seed density on germination and plant growth were investigated. Surface sterilization of barley seeds had a negative impact on germination. Increasing the ethanol concentration and/or the bleach concentration reduced the germination percentage. Barley seeds were first germinated in water in the hydroponics system. The seedlings then received wastewater from an aquaculture system stocked with Arctic charr. During the experiment, the crops grew rapidly and fairly uniformly and showed no signs of mineral deficiency or disease. The average crop height at harvest was 25.5 cm and the yield varied from 25 to 59 t haˉ1, depending on the seed density. The hydroponically grown barley was able to significantly reduce the pollution load of the aquaculture wastewater. The TS, COD, NH4+-N, NO2--N, NO3--N, and PO43--P reductions ranged from 52.7 to 60.5%, from 72.9 to 83.1%, from 76.0 to 76.0%, from 97.6 to 99.2%, from 76.9 to 81.6% and from 87.1 to 95.1%, respectively. However, the effluent produced from the hydroponics system had slightly higher levels of TS (420-485 mg Lˉ1 than the 480 mg Lˉ1 recommended for aquatic animals. A sedimentation/filtration unit should be added to the hydroponics system.

  2. Nanolipoprotein particles and related methods and systems for protein capture, solubilization, and/or purification

    Energy Technology Data Exchange (ETDEWEB)

    Chromy, Brett A.; Henderson, Paul; Hoeprich, Jr, Paul D.

    2016-10-04

    Provided herein are methods and systems for assembling, solubilizing and/or purifying a membrane associated protein in a nanolipoprotein particle, which comprise a temperature transition cycle performed in presence of a detergent, wherein during the temperature transition cycle the nanolipoprotein components are brought to a temperature above and below the gel to liquid crystalling transition temperature of the membrane forming lipid of the nanolipoprotein particle.

  3. Design approaches for a cycling adsorbent/photocatalyst system for indoor air purification: formaldehyde example.

    Science.gov (United States)

    Chin, Paul; Ollis, David F

    2008-04-01

    A kinetic model for a cycling adsorbent/photocatalyst combination for formaldehyde removal in indoor air (Chin et al. J. Catalysis 2006, 237, 29-37) was previously developed in our lab, demonstrating agreement with lab-scale batch operation data of other researchers (Shiraishi et al. Chem. Engineer. Sci. 2003, 58, 929-934). Model parameters evaluated included adsorption equilibrium and rate constants for the adsorbent (activated carbon) honeycomb rotor, and catalytic rate constant for pseudo-first-order formaldehyde destruction in the titanium dioxide photoreactor. This paper explores design consequences for this novel system. In particular, the batch parameter values are used to model both adsorbent and photocatalyst behavior for continuous operation in typical residential home challenges. Design variables, including realistic make-up air fraction, adsorbent honeycomb rotation speed, and formaldehyde source emission rate, are considered to evaluate the ability of the system to achieve World Health Organization pollutant guidelines. In all circumstances, the size of the required rotating adsorbent bed and photoreactor for single-stage operation and the resultant formaldehyde concentration in the home are calculated. The ability of how well such a system might be accommodated within the typical dimensions of commercial ventilation ducts is also considered.

  4. Applications of Water Fog Purification System in the Flare System%水雾净化系统在火炬回收系统中的应用

    Institute of Scientific and Technical Information of China (English)

    白丽影; 熊梦林

    2012-01-01

    The gas from refineries contains hydrogen sulfide, fine coke, catalyst fines, rusty stain, which makes equipments and pipelines corrode, leak, block and destroy to seriously influence safe operation of the flare system. In this paper, the working principle of water fog purification system was introduced as well as its application in the flare system, and some suggestions were put forward to solve the problems in the operation, which can ensure the best work condition of the system.%炼油装置产生的瓦斯气中含有的硫化氢、焦粉、催化剂粉末、锈渣等成分,极易造成设备、管线腐蚀泄漏、堵塞损坏,给火炬回收系统的安全运行带来严重影响.针对某炼油厂增上的水雾净化系统的工作原理和在火炬回收系统的应用情况进行了介绍,针对运行中出现的问题提出了整改建议,以确保实现系统最佳运行工况.

  5. EMC of Electrical Systems - Electromagnetic Coupling ( Part II

    Directory of Open Access Journals (Sweden)

    KOVACOVA, I.

    2007-04-01

    Full Text Available The paper deals with the general analysis of one part of the electromagnetic compatibility (EMC problem - the electromagnetic coupling applied in the field of power electrical systems. The verification simulation analyses and practical measurements of the electromagnetic coupling, which are confirming the correctness of results obtained from theoretical analyses (part I., are presented in part II. So they can be used for predictive stating of EMC quality of individual new electrotechnical products.

  6. LCLS-II high power RF system overview and progress

    Energy Technology Data Exchange (ETDEWEB)

    Yeremian, Anahid Dian

    2015-10-07

    A second X-ray free electron laser facility, LCLS-II, will be constructed at SLAC. LCLS-II is based on a 1.3 GHz, 4 GeV, continuous-wave (CW) superconducting linear accelerator, to be installed in the first kilometer of the SLAC tunnel. Multiple types of high power RF (HPRF) sources will be used to power different systems on LCLS-II. The main 1.3 GHz linac will be powered by 280 1.3 GHz, 3.8 kW solid state amplifier (SSA) sources. The normal conducting buncher in the injector will use four more SSAs identical to the linac SSAs but run at 2 kW. Two 185.7 MHz, 60 kW sources will power the photocathode dual-feed RF gun. A third harmonic linac section, included for linearizing the bunch energy spread before the first bunch compressor, will require sixteen 3.9 GHz sources at about 1 kW CW. A description and an update on all the HPRF sources of LCLS-II and their implementation is the subject of this paper.

  7. Belle II public and private cloud management in VMDIRAC system.

    Science.gov (United States)

    Grzymkowski, Rafa; Hara, Takanori; Belle computing Group, II

    2015-12-01

    The role of cloud computing technology in the distributed computing for HEP experiments grows rapidly. Some experiments (Atlas, BES-III, LHCb) already exploit private and public cloud resources for the data processing. Future experiments such as Belle II or upgraded LHC experiments will largely rely on the availability of cloud resources and therefore their computing models have to be adjusted to the specific features of cloud environment, in particular to the on-demand computing paradigm. Belle II experiment at SuperKEKB will start physics run in 2017. Belle II computing requirements are challenging. The data size at the level of hundred PB is expected after several years of operation, around 2020. The baseline solution selected for distributed processing is the DIRAC system. DIRAC can handle variety of computing resources including Grids, Clouds and independent clusters. Cloud resources can be connected by VMDIRAC module through public interfaces. In particular the mechanism of dynamic activation of new virtual machines with reserved job slots for new tasks in case of an increasing demand for computing resources is introduced. This work is focused on VMDIRAC interaction with public (Amazon EC2) and private (CC1) cloud. The solution applied by Belle II experiment and the experience from Monte Carlo production campaigns will be presented. Updated computation costs for different use cases will be shown.

  8. Phase system selection with fractional factorial design for purification of recombinant cyanovirin-N from a hydroponic culture medium using centrifugal partition chromatography.

    Science.gov (United States)

    Grudzień, Łukasz; Madeira, Luisa; Fisher, Derek; Ma, Julian; Garrard, Ian

    2013-04-12

    Centrifugal partition chromatography (CPC) with an aqueous two-phase system (ATPS) was used to purify recombinant cyanovirin-N (CV-N) from other proteins which were co-secreted into a hydroponic plant medium in a rhizosecretion process. To achieve satisfactory protein concentration, the purification was preceded by ultrafiltration performed on a 5 kDa filter. ATPS, because of their gentle nature, were selected as the phase system for CPC. A systematic phase system selection was applied. This involved studying the effect of seven parameters of ATPS: polymer type, salt type, the polymer and salt concentration, the polymer molecular weight, pH, and presence of two additional salts; NaCl and NaClO4, which all together gave 320 combinations. design of experiment (DoE) software allowed the reduction of this number to 46. Having tested partitioning of cyanovirin-N and impurities in 46 ATPS, the three best potential phase systems generated by the programme were then tested on the CPC. Out of these three, 13/13% PEG4000 sodium phosphate, pH 3.0, proved to be most effective phase system in the purification of cyanovirin-N, judged by ELISA and SDS-PAGE analysis, as it eliminated most of the impurities from the final cyanovirin-N preparation.

  9. Liquid Argon Dielectric Breakdown Studies with the MicroBooNE Purification System

    Energy Technology Data Exchange (ETDEWEB)

    Acciarri, R. [Fermilab; Carls, B. [Fermilab; James, C. [Fermilab; Johnson, B. [Fermilab; Jostlein, H. [Fermilab; Lockwitz, S. [Fermilab; Lundberg, B. [Fermilab; Raaf, J. L. [Fermilab; Rameika, R. [Fermilab; Rebel, B. [Fermilab; Zeller, G. P. [Fermilab; Zuckerbrot, M. [Fermilab

    2014-11-04

    The proliferation of liquid argon time projection chamber detectors makes the characterization of the dielectric properties of liquid argon a critical task. To improve understanding of these properties, a systematic study of the breakdown electric field in liquid argon was conducted using a dedicated cryostat connected to the MicroBooNE cryogenic system at Fermilab. An electrode sphere-plate geometry was implemented using spheres with diameters of 1.3 mm, 5.0 mm, and 76 mm. The MicroBooNE cryogenic system allowed measurements to be taken at a variety of electronegative contamination levels ranging from a few parts-per-million to tens of parts-per-trillion. The cathode-anode distance was varied from 0.1 mm to 2.5 cm. The results demonstrate a geometric dependence of the electric field strength at breakdown. This study is the first time that the dependence of the breakdown field on stressed cathode area has been shown for liquid argon.

  10. Liquid Argon Dielectric Breakdown Studies with the MicroBooNE Purification System

    CERN Document Server

    Acciarri, R; James, C; Johnson, B; Jostlein, H; Lockwitz, S; Lundberg, B; Raaf, J L; Rameika, R; Rebel, B; Zeller, G P; Zuckerbrot, M

    2014-01-01

    The proliferation of liquid argon time projection chamber detectors makes the characterization of the dielectric properties of liquid argon a critical task. To improve understanding of these properties, a systematic study of the breakdown electric field in liquid argon was conducted using a dedicated cryostat connected to the MicroBooNE cryogenic system at Fermilab. An electrode sphere-plate geometry was implemented using spheres with diameters of 1.3 mm, 5.0 mm, and 76 mm. The MicroBooNE cryogenic system allowed measurements to be taken at a variety of electronegative contamination levels ranging from a few parts-per-million to tens of parts-per- trillion. The cathode-anode distance was varied from 0.1 mm to 2.5 cm. The results demonstrate a geometric dependence of the electric field strength at breakdown. This study is the first time that the dependence of the breakdown field on stressed cathode area has been shown for liquid argon.

  11. Liquid argon dielectric breakdown studies with the MicroBooNE purification system

    Energy Technology Data Exchange (ETDEWEB)

    Acciarri, R.; Carls, B.; James, C.; Johnson, B.; Jostlein, H.; Lockwitz, S.; Lundberg, B.; Raaf, J. L.; Rameika, R.; Rebel, B.; Zeller, G. P.; Zuckerbrot, M.

    2014-11-01

    The proliferation of liquid argon time projection chamber detectors makes the characterization of the dielectric properties of liquid argon a critical task. To improve understanding of these properties, a systematic study of the breakdown electric field in liquid argon was conducted using a dedicated cryostat connected to the MicroBooNE cryogenic system at Fermilab. An electrode sphere-plate geometry was implemented using spheres with diameters of 1.3 mm, 5.0 mm, and 76 mm. The MicroBooNE cryogenic system allowed measurements to be taken at a variety of electronegative contamination levels ranging from a few parts-per-million to tens of parts-per-trillion. The cathode-anode distance was varied from 0.1 mm to 2.5 cm. The results demonstrate a geometric dependence of the electric field strength at breakdown. This study is the first time that the dependence of the breakdown field on stressed cathode area has been shown for liquid argon.

  12. Thermal decomposition of solid solutions in systems of Fe(II), Co(II), and Ni(II) hydrogen maleates with the formation of bimetallic nanoparticles

    Science.gov (United States)

    Yudanova, L. I.; Logvinenko, V. A.; Sheludyakova, L. A.; Ishchenko, A. V.; Rudina, N. A.

    2017-01-01

    XRD phase analysis and thermal analysis are used to confirm the formation of a continuous series of solid solutions in which one cation is substituted for another in the systems Co(II) hydrogen maleate-Ni(II) hydrogen maleate; Fe(II) hydrogen maleate-Co(II) hydrogen maleate; and Fe(II) hydrogen maleate-Ni(II) hydrogen maleate. The unit cell volume of these solid solutions is shown to depend linearly on their composition. The linear character of changes in the initial temperatures of dehydration and thermal decomposition is established. Using the example of the first of these systems, it is shown that when heated, bimetallic nanoparticles embedded in the polymeric matrix of composites obtained via the thermal decomposition of solid solutions of hydrogen maleates undergo a second-order phase transition, resulting in decomposition of the solid solutions of metals at the Curie temperature.

  13. Ethernet-Based DAQ System for QUIET-II Detectors

    Science.gov (United States)

    Nagai, M.; Ishidoshiro, K.; Higuchi, T.; Ikeno, M.; Hasegawa, M.; Hazumi, M.; Tajima, O.; Tanaka, M.; Uchida, T.

    2012-06-01

    The B-modes in cosmic microwave background polarization are a smoking gun for the inflationary universe. For the detection of the B-modes, having a large detector array is a generic approach since the B-modes is so faint pattern ( T b≲0.1 μK). The Q/U Imaging ExperimenT Phase-II (QUIET-II) is proposed to search the B-modes, using an array with 500 HEMT-based polarimeters. Each polarimeter element has 4-outputs, therefore we have to manage 2000 channels in total. We developed a scalable DAQ system based on TCP/Ethernet for QUIET-II. The DAQ system is composed of the polarimeters, ADC boards, a Master Clock and a control computer (PC). The analog signals from the polarimeters are digitized on the ADC boards. On-board demodulation, which synchronizes the phase flip modulations on the polarimeter, extracts the polarized components in the digitized signal. The Master Clock distributes all necessary clocks to the ADC boards as well as the polarimeters. This scheme guarantees the synchronization of the modulations and demodulations. We employed Ethernet-based communication scheme between the data collection program (Collector) on the PC and the ADC boards as well as the Master Clock. Such an Ethernet-based communication scheme allows us to construct a simple structure of the upper level software, which results in the high scalability to increase the number of channels. All basic functions and requirements are confirmed by the laboratory tests; demonstration with test signals as well as the signals from the polarimeters, measurements of the data transfer rate, and the synchronous operation with two ADC boards. Therefore, the DAQ system is confirmed to be suitable for QUIET-II.

  14. The type II secretion system: biogenesis, molecular architecture and mechanism.

    Science.gov (United States)

    Korotkov, Konstantin V; Sandkvist, Maria; Hol, Wim G J

    2012-04-02

    Many gram-negative bacteria use the sophisticated type II secretion system (T2SS) to translocate a wide range of proteins from the periplasm across the outer membrane. The inner-membrane platform of the T2SS is the nexus of the system and orchestrates the secretion process through its interactions with the periplasmic filamentous pseudopilus, the dodecameric outer-membrane complex and a cytoplasmic secretion ATPase. Here, recent structural and biochemical information is reviewed to describe our current knowledge of the biogenesis and architecture of the T2SS and its mechanism of action.

  15. Physics Detector Simulation Facility Phase II system software description

    Energy Technology Data Exchange (ETDEWEB)

    Scipioni, B.; Allen, J.; Chang, C.; Huang, J.; Liu, J.; Mestad, S.; Pan, J.; Marquez, M.; Estep, P.

    1993-05-01

    This paper presents the Physics Detector Simulation Facility (PDSF) Phase II system software. A key element in the design of a distributed computing environment for the PDSF has been the separation and distribution of the major functions. The facility has been designed to support batch and interactive processing, and to incorporate the file and tape storage systems. By distributing these functions, it is often possible to provide higher throughput and resource availability. Similarly, the design is intended to exploit event-level parallelism in an open distributed environment.

  16. Countercurrent chromatographic separation and purification of various ribonucleases using a small-scale cross-axis coil planet centrifuge with aqueous-aqueous polymer phase systems.

    Science.gov (United States)

    Shinomiya, Kazufusa; Kobayashi, Hiroko; Motoyoshi, Naomi; Inokuchi, Norio; Nakagomi, Kazuya; Ito, Yoichiro

    2009-04-01

    Countercurrent chromatographic (CCC) separation and purification of various ribonucleases (RNases) was performed using the small-scale cross-axis coil planet centrifuge (X-axis CPC) with aqueous-aqueous polymer phase systems. RNases B and A were well resolved from each other with an aqueous-aqueous polymer phase system composed of 12.5% (w/w) polyethylene glycol (PEG) 1000 and 12.5% (w/w) dibasic potassium phosphate (pH 9.2) as the mobile lower phase. The commercial RNase A samples obtained from three different companies were also highly purified using the 16.0% (w/w) PEG 1000-6.3% (w/w) dibasic potassium phosphate-6.3% (w/w) monobasic potassium phosphate system (pH 6.6) using the upper phase as the mobile phase. Recombinant RNase Po(1), an RNase T(1) family enzyme, was further successfully separated from the crude extract using the same solvent system with the lower phase used as the mobile phase. The RNase activities were well preserved during the CCC separation. The overall results demonstrate that the small-scale X-axis CPC is useful for a simple and rapid purification of various RNases in a preparative-scale.

  17. Centrifugal LabTube platform for fully automated DNA purification and LAMP amplification based on an integrated, low-cost heating system.

    Science.gov (United States)

    Hoehl, Melanie M; Weißert, Michael; Dannenberg, Arne; Nesch, Thomas; Paust, Nils; von Stetten, Felix; Zengerle, Roland; Slocum, Alexander H; Steigert, Juergen

    2014-06-01

    This paper introduces a disposable battery-driven heating system for loop-mediated isothermal DNA amplification (LAMP) inside a centrifugally-driven DNA purification platform (LabTube). We demonstrate LabTube-based fully automated DNA purification of as low as 100 cell-equivalents of verotoxin-producing Escherichia coli (VTEC) in water, milk and apple juice in a laboratory centrifuge, followed by integrated and automated LAMP amplification with a reduction of hands-on time from 45 to 1 min. The heating system consists of two parallel SMD thick film resistors and a NTC as heating and temperature sensing elements. They are driven by a 3 V battery and controlled by a microcontroller. The LAMP reagents are stored in the elution chamber and the amplification starts immediately after the eluate is purged into the chamber. The LabTube, including a microcontroller-based heating system, demonstrates contamination-free and automated sample-to-answer nucleic acid testing within a laboratory centrifuge. The heating system can be easily parallelized within one LabTube and it is deployable for a variety of heating and electrical applications.

  18. Purification and concentration of alphavirus.

    Science.gov (United States)

    Lundstrom, Kenneth

    2012-07-01

    The alphaviruses Semliki Forest virus and Sindbis virus have been used frequently as expression vectors in vitro and in vivo. Usually, these systems consist of replication-deficient vectors that require a helper vector for packaging of recombinant particles. Replication-proficient vectors have also been engineered. Alphaviral vectors can be used as nucleic-acid-based vectors (DNA and RNA) or infectious particles. High-titer viral production is achieved in alphaviruses facilitates studies in mammalian and nonmammalian cell lines, primary cells in culture, and in vivo. The strong preference for expression in neuronal cells has made alphaviruses particularly useful in neurobiological studies. Unfortunately, their strong cytotoxic effect on host cells, relatively short-term transient expression patterns, and the reasonably high cost of viral production remain drawbacks. However, novel mutant alphaviruses have showed reduced cytotoxicity and prolonged expression. Membrane proteins (which are generally difficult to express at high levels in recombinant systems) have generated high yields and facilitate applications in structural biology. Alphaviruses have also been applied in vaccine development and gene therapy. Generally, purification or concentration of alphaviruses is not necessary. However, for instance, the medium derived from baby hamster kidney cells is toxic to primary neurons in culture. Including a purification step substantially improves the survival of the transduced neurons. Viral concentration and purification may also be advantageous for in vivo studies in animal models and are mandatory for clinical applications. This protocol describes three methods for purification and concentration of alphavirus.

  19. Air purification from TCE and PCE contamination in a hybrid bioreactors and biofilter integrated system.

    Science.gov (United States)

    Tabernacka, Agnieszka; Zborowska, Ewa; Lebkowska, Maria; Borawski, Maciej

    2014-01-15

    A two-stage waste air treatment system, consisting of hybrid bioreactors (modified bioscrubbers) and a biofilter, was used to treat waste air containing chlorinated ethenes - trichloroethylene (TCE) and tetrachloroethylene (PCE). The bioreactor was operated with loadings in the range 0.46-5.50gm(-3)h(-1) for TCE and 2.16-9.02gm(-3)h(-1) for PCE. The biofilter loadings were in the range 0.1-0.97gm(-3)h(-1) for TCE and 0.2-2.12gm(-3)h(-1) for PCE. Under low pollutant loadings, the efficiency of TCE elimination was 23-25% in the bioreactor and 54-70% in the biofilter. The efficiency of PCE elimination was 44-60% in the bioreactor and 50-75% in the biofilter. The best results for the bioreactor were observed one week after the pollutant loading was increased. However, the process did not stabilize. In the next seven days contaminant removal efficiency, enzymatic activity and biomass content were all diminished.

  20. Purification and in situ immobilization of papain with aqueous two-phase system.

    Science.gov (United States)

    Li, Mingliang; Su, Erzheng; You, Pengyong; Gong, Xiangyu; Sun, Ming; Xu, Diansheng; Wei, Dongzhi

    2010-12-13

    Papain was purified from spray-dried Carica papaya latex using aqueous two-phase system (ATPS). Then it was recovered from PEG phase by in situ immobilization or preparing cross-linked enzyme aggregates (CLEAs). The Plackett-Burman design and the central composite design (CCD) together with the response surface methodology (RSM) were used to optimize the APTS processes. The highly purified papain (96-100%) was achieved under the optimized conditions: 40% (w/w) 15 mg/ml enzyme solution, 14.33-17.65% (w/w) PEG 6000, 14.27-14.42% (w/w) NaH2PO4/K2HPO4 and pH 5.77-6.30 at 20°C. An in situ enzyme immobilization approach, carried out by directly dispersing aminated supports and chitosan beads into the PEG phase, was investigated to recover papain, in which a high immobilization yield (>90%) and activity recovery (>40%) was obtained. Moreover, CLEAs were successfully used in recovering papain from PEG phase with a hydrolytic activity hundreds times higher than the carrier-bound immobilized papain.

  1. Phospholipid polymer-based antibody immobilization for cell rolling surfaces in stem cell purification system.

    Science.gov (United States)

    Mahara, Atsushi; Chen, Hao; Ishihara, Kazuhiko; Yamaoka, Tetsuji

    2014-01-01

    We previously developed an antibody-conjugated cell rolling column that successfully separates stem cell subpopulations depending on the cell surface marker density, but a large amount of the injected cells were retained in the column because of non-specific interactions. In this study, an amphiphilic copolymer, poly[2-methacryloyloxyethyl phosphorylcholine (MPC)-co-n-butyl methacrylate (nBMA)-co-N-vinyl formamide (NVf)], with phospholipid polar side groups was designed as a novel antibody-immobilizing modifier. The formamide groups in NVf units were converted to active maleimide groups. A plastic flow microfluidic chamber was coated with the copolymers, and a reduced anti-CD90 antibody was immobilized. The adipose tissue-derived stem cells isolated from the rat were injected into the flow chamber, and their rolling behavior was observed under a microscope with a high-speed camera. Non-specific cell adhesion was reduced strongly by means of this immobilization method because of the MPC unit, resulting in a high percentage of rolling cells. These results demonstrate that a surface coated with phospholipid polar groups can be used in an effective stem cell separation system based on the cell rolling process.

  2. Expression and Purification of C-Peptide Containing Insulin Using Pichia pastoris Expression System

    Directory of Open Access Journals (Sweden)

    Mohammed N. Baeshen

    2016-01-01

    Full Text Available Increase in the incidence of Insulin Dependent Diabetes Mellitus (IDDM among people from developed and developing countries has created a large global market for insulin. Moreover, exploration of new methods for insulin delivery including oral or inhalation route which require very high doses would further increase the demand of cost-effective recombinant insulin. Various bacterial and yeast strains have been optimized to overproduce important biopharmaceuticals. One of the approaches we have taken is the production of recombinant human insulin along with C-peptide in yeast Pichia pastoris. We procured a cDNA clone of insulin from Origene Inc., USA. Insulin cDNA was PCR amplified and cloned into yeast vector pPICZ-α. Cloned insulin cDNA was confirmed by restriction analysis and DNA sequencing. pPICZ-α-insulin clone was transformed into Pichia pastoris SuperMan5 strain. Several Zeocin resistant clones were obtained and integration of insulin cDNA in Pichia genome was confirmed by PCR using insulin specific primers. Expression of insulin in Pichia clones was confirmed by ELISA, SDS-PAGE, and Western blot analysis. In vivo efficacy studies in streptozotocin induced diabetic mice confirmed the activity of recombinant insulin. In conclusion, a biologically active human proinsulin along with C-peptide was expressed at high level using Pichia pastoris expression system.

  3. Final Safety Analysis Addenda to Hazards Summary Report, Experimental Breeder Reactor II (EBR-II): upgrading of plant protection system. Volume II

    Energy Technology Data Exchange (ETDEWEB)

    Allen, N. L.; Keeton, J. M.; Sackett, J. I. [comps.

    1980-06-01

    This report is the second in a series of compilations of the formal Final Safety Analysis Addenda (FSAA`s) to the EBR-II Hazard Summary Report and Addendum. Sections 2 and 3 are edited versions of the original FSAA`s prepared in support of certain modifications to the reactor-shutdown-system portion of the EBR-II plant-protection system. Section 4 is an edited version of the original FSAA prepared in support of certain modifications to a system classified as an engineered safety feature. These sections describe the pre- and postmodification system, the rationale for the modification, and required supporting safety analysis. Section 5 provides an updated description and analysis of the EBR-II emergency power system. Section 6 summarizes all significant modifications to the EBR-II plant-protection system to date.

  4. Urban Integrated Industrial Cogeneration Systems Analysis. Phase II final report

    Energy Technology Data Exchange (ETDEWEB)

    1984-01-01

    Through the Urban Integrated Industrial Cogeneration Systems Analysis (UIICSA), the City of Chicago embarked upon an ambitious effort to identify the measure the overall industrial cogeneration market in the city and to evaluate in detail the most promising market opportunities. This report discusses the background of the work completed during Phase II of the UIICSA and presents the results of economic feasibility studies conducted for three potential cogeneration sites in Chicago. Phase II focused on the feasibility of cogeneration at the three most promising sites: the Stockyards and Calumet industrial areas, and the Ford City commercial/industrial complex. Each feasibility case study considered the energy load requirements of the existing facilities at the site and the potential for attracting and serving new growth in the area. Alternative fuels and technologies, and ownership and financing options were also incorporated into the case studies. Finally, site specific considerations such as development incentives, zoning and building code restrictions and environmental requirements were investigated.

  5. Digital Speed Control System Design for Air Purification Equipment%空气净化装置数字化调速控制系统设计

    Institute of Scientific and Technical Information of China (English)

    石宝松; 张伟; 刘剑; 韩振伟

    2014-01-01

    地平式激光跟踪发射系统为保证其光路洁净,须进行正压防尘处理。针对外购空气净化设备仅能采用手动风速调节,不能实现计算机远程控制的不足,研发了空气净化设备数字化调速控制系统,以实现对空气净化装置的远程控制与状态监测。本设计采用光电隔离单相交流调压模块实现对风机的驱动,采用霍尔电流传感器实现对风机的电流测量,并采用内部集成A/D转换器和D/A转换器的单片机通过RS422总线接收控制指令,完成对调压模块的输出功率控制和风机电流的实时采集。本系统设计简洁、控制方便、运行可靠、易于维护,实现了设备管理计算机对空气净化设备风速的远程控制。%Horizon emitting laser tracking system to ensure its optical path cleaning, shall be positive dust treatment. For the lack of air purification equipment purchased only with manual speed adjustment and the remote control of a computer can not be achieved, we have developed an air purification equipment digital speed control system to achieve the remote control and status monitoring of air purification device. The design uses a optically isolated single-phase AC voltage regulator module for fan drive,uses a Hall current sensors to achieve the fan current measurement,and uses a microcontroller, which integrates a A/D converter and a D/A converter, to receive the control command via the RS422 bus, to complete the output power control of voltage regulator module, and to realize the real-time collection of fan’ s current. The system is designed to be simple, easy to control, reliable to operate and easy to maintain and realizes the remote control of a device management computer on the wind speed of air purification equipment.

  6. Evaluation of Laser Stabilization and Imaging Systems for LCLS-II - Oral Presentation

    Energy Technology Data Exchange (ETDEWEB)

    Barry, Matthew; /Auburn U.

    2015-08-19

    This presentation covers data collected on two commercial laser stabilization systems, Guidestar-II and MRC, and two optical imaging systems. Additionally, general information about LCLS-II and how to go about continuing-testing is covered.

  7. Modeling and Analysis of NGC System using Ptolemy II

    Directory of Open Access Journals (Sweden)

    Archana Sreekumar

    2015-09-01

    Full Text Available Model based system design has been used in real time embedded systems for validating and testing during the development lifecycle. Computation models - synchronous dataflow model (SDF and Discrete Event (DE have been used and finite state machine has been integrated with SDF and Discrete Event (DE modeling domains for simulating the functionalities in the system. Here a case study of resource augmented Navigation, Guidance and Control unit of onboard computers in satellite launch vehicle has been selected as a frame work and fault tolerant algorithm has been modeled and simulated with Ptolemy II. Feasibility of the scheduling of the fault tolerant algorithm has been analyzed and dependencies existing between different components and processes in the system have been investigated. The future work consists of modeling original functionality of NGC units inside each state of FSM and can be validated for the correct performance. Non-deterministic communication and clock drifts can be accounted into the model.

  8. PHASE II VAULT TESTING OF THE ARGONNE RFID SYSTEM

    Energy Technology Data Exchange (ETDEWEB)

    Willoner, T.; Turlington, R.; Koenig, R.

    2012-06-25

    The U.S. Department of Energy (DOE) (Environmental Management [EM], Office of Packaging and Transportation [EM-45]) Packaging and Certification Program (DOE PCP) has developed a Radio Frequency Identification (RFID) tracking and monitoring system, called ARG-US, for the management of nuclear materials packages during transportation and storage. The performance of the ARG-US RFID equipment and system has been fully tested in two demonstration projects in April 2008 and August 2009. With the strong support of DOE-SR and DOE PCP, a field testing program was completed in Savannah River Site's K-Area Material Storage (KAMS) Facility, an active Category I Plutonium Storage Facility, in 2010. As the next step (Phase II) of continued vault testing for the ARG-US system, the Savannah River Site K Area Material Storage facility has placed the ARG-US RFIDs into the 910B storage vault for operational testing. This latest version (Mark III) of the Argonne RFID system now has the capability to measure radiation dose and dose rate. This paper will report field testing progress of the ARG-US RFID equipment in KAMS, the operability and reliability trend results associated with the applications of the system, and discuss the potential benefits in enhancing safety, security and materials accountability. The purpose of this Phase II K Area test is to verify the accuracy of the radiation monitoring and proper functionality of the ARG-US RFID equipment and system under a realistic environment in the KAMS facility. Deploying the ARG-US RFID system leads to a reduced need for manned surveillance and increased inventory periods by providing real-time access to status and event history traceability, including environmental condition monitoring and radiation monitoring. The successful completion of the testing program will provide field data to support a future development and testing. This will increase Operation efficiency and cost effectiveness for vault operation. As the next step

  9. Copper(II)-8-hydroxquinoline coprecipitation system for preconcentration and separation of cobalt(II) and manganese(II) in real samples.

    Science.gov (United States)

    Soylak, Mustafa; Kaya, Betul; Tuzen, Mustafa

    2007-08-25

    A separation-preconcentration procedure based on the coprecipitation of cobalt(II) and manganese(II) ions with copper(II)-8-hydroxquinoline system has been developed. The analytical parameters including pH, amount of copper(II) as carrier element, amount of 8-hydroxquinoline, sample volume, etc., was investigated for the quantitative recoveries of Co(II) and Mn(II). No interferic effects were observed from the concomitant ions which are present in real samples. The detection limits for analyte ions by three sigma criteria were 0.86microgL(-1) for cobalt and 0.98microgL(-1) for manganese. The validation of the presented preconcentration procedure was performed by the analysis of NIST SRM 2711 Montana soil and GBW 07605 Tea certified reference materials. The procedure presented was applied to the analyte contents of real samples including natural waters and some food samples with successfully analytical results.

  10. Bioinspired Materials for Water Purification

    Directory of Open Access Journals (Sweden)

    Alfredo Gonzalez-Perez

    2016-06-01

    Full Text Available Water scarcity issues associated with inadequate access to clean water and sanitation is a ubiquitous problem occurring globally. Addressing future challenges will require a combination of new technological development in water purification and environmental remediation technology with suitable conservation policies. In this scenario, new bioinspired materials will play a pivotal role in the development of more efficient and environmentally friendly solutions. The role of amphiphilic self-assembly on the fabrication of new biomimetic membranes for membrane separation like reverse osmosis is emphasized. Mesoporous support materials for semiconductor growth in the photocatalytic degradation of pollutants and new carriers for immobilization of bacteria in bioreactors are used in the removal and processing of different kind of water pollutants like heavy metals. Obstacles to improve and optimize the fabrication as well as a better understanding of their performance in small-scale and pilot purification systems need to be addressed. However, it is expected that these new biomimetic materials will find their way into the current water purification technologies to improve their purification/removal performance in a cost-effective and environmentally friendly way.

  11. Earth System Grid II, Turning Climate Datasets into Community Resources

    Energy Technology Data Exchange (ETDEWEB)

    Middleton, Don

    2006-08-01

    The Earth System Grid (ESG) II project, funded by the Department of Energy’s Scientific Discovery through Advanced Computing program, has transformed climate data into community resources. ESG II has accomplished this goal by creating a virtual collaborative environment that links climate centers and users around the world to models and data via a computing Grid, which is based on the Department of Energy’s supercomputing resources and the Internet. Our project’s success stems from partnerships between climate researchers and computer scientists to advance basic and applied research in the terrestrial, atmospheric, and oceanic sciences. By interfacing with other climate science projects, we have learned that commonly used methods to manage and remotely distribute data among related groups lack infrastructure and under-utilize existing technologies. Knowledge and expertise gained from ESG II have helped the climate community plan strategies to manage a rapidly growing data environment more effectively. Moreover, approaches and technologies developed under the ESG project have impacted datasimulation integration in other disciplines, such as astrophysics, molecular biology and materials science.

  12. AgIIS, Agricultural Irrigation Imaging System, design and application

    Science.gov (United States)

    Haberland, Julio Andres

    Remote sensing is a tool that is increasingly used in agriculture for crop management purposes. A ground-based remote sensing data acquisition system was designed, constructed, and implemented to collect high spatial and temporal resolution data in irrigated agriculture. The system was composed of a rail that mounts on a linear move irrigation machine, and a small cart that runs back and forth on the rail. The cart was equipped with a sensors package that measured reflectance in four discrete wavelengths (550 nm, 660 nm, 720 nm, and 810 nm, all 10 nm bandwidth) and an infrared thermometer. A global positioning system and triggers on the rail indicated cart position. The data was postprocessed in order to generate vegetation maps, N and water status maps and other indices relevant for site-specific crop management. A geographic information system (GIS) was used to generate images of the field on any desired day. The system was named AgIIS (A&barbelow;gricultural I&barbelow;rrigation I&barbelow;maging S&barbelow;ystem). This ground based remote sensing acquisition system was developed at the Agricultural and Biosystems Engineering Department at the University of Arizona in conjunction with the U.S. Water Conservation Laboratory in Phoenix, as part of a cooperative study primarily funded by the Idaho National Environmental and Engineering Laboratory. A second phase of the study utilized data acquired with AgIIS during the 1999 cotton growing season to model petiole nitrate (PNO3 -) and total leaf N. A latin square experimental design with optimal and low water and optimal and low N was used to evaluate N status under water and no water stress conditions. Multivariable models were generated with neural networks (NN) and multilinear regression (MLR). Single variable models were generated from chlorophyll meter readings (SPAD) and from the Canopy Chlorophyll Content Index (CCCI). All models were evaluated against observed PNO3- and total leaf N levels. The NN models

  13. Effects of indoor air purification by an air cleaning system (Koala technology) on semen parameters in male factor infertility: results of a pilot study.

    Science.gov (United States)

    Paradisi, R; Vanella, S; Barzanti, R; Cani, C; Battaglia, C; Seracchioli, R; Venturoli, S

    2009-06-01

    A number of studies indicated a clear decline in semen quality in the past 30-50 years and there is accumulating evidence that this decline might result from exposure to high levels of air pollution. To examine the impact of environment on male reproductive ability, we undertook for the first time a pilot study on semen quality of infertile men exposed to purification of indoor air. Ten subjects with a history of unexplained male infertility and poor semen quality were exposed for at least 1 year to a cleaning indoor air system (Koala technology). The key feature of this air purifier is the unique innovative multiple filtering system. The treatment of total purification of indoor air showed neither improvements in semen parameters nor variation in reproductive hormones (P = N.S.), but induced an evident increase (P indoor air does not seem enough to improve semen quality, although the increase in leucocytic concentrations could indicate an activation of the role of immunosurveillance in a purified indoor air environment.

  14. Evaluation of a new preparative supercritical fluid chromatography system for compound library purification: the TharSFC SFC-MS Prep-100 system.

    Science.gov (United States)

    Ebinger, Katalin; Weller, Harold N; Kiplinger, Jeffrey; Lefebvre, Paul

    2011-06-01

    Preparative HPLC-MS is often the method of choice for purification of small amounts (<100mg) of diverse new molecules, such as compound libraries for drug discovery. The method is robust, well proven, and widely applicable. In contrast, preparative supercritical fluid chromatography coupled with mass spectrometry (SFC-MS) has seen only slow acceptance for the same application--despite some potential scientific and economic advantages. One of the reasons for slow adoption of SFC-MS is the lack of well-proven, robust, and commercially available instrumentation. In early 2009, TharSFC (a Waters Company, Pittsburgh, PA) introduced a new fully integrated system for preparative SFC-MS: The SFC-MS Prep-100. We report herein an objective evaluation of the SFC-MS Prep-100, including tests for pump and autosampler performance, sample recovery, sample carryover, fraction triggering, detector/fraction collector synchronization, and overall robustness. Our results suggest that the SFC-MS Prep-100 represents a significant advance over previous generation instrumentation.

  15. Controlling Points of Air Purification System in Design and Construction Process%净化空调系统在设计施工过程中的控制要点

    Institute of Scientific and Technical Information of China (English)

    刘静

    2014-01-01

    随着高新技术的发展,对生产环境的洁净要求也越来越严格,净化空调的应用越来越广泛。该文在阐述洁净等级的划分之后,根据实际经验提出净化空调系统设计存在的问题,并分析了系统施工过程应注意的问题。%With the development of high and new technology, requirements on production environment are getting increasingly stricter, and application of air purification system is also getting increasingly wider. The classification of purification level is elaborated, the existing problems in air purification sys-tem design are presented in accordance with actual experience and some attention worth issues in construction process are also analyzed.

  16. Microscopic anatomy of pycnogonida: II. Digestive system. III. Excretory system.

    Science.gov (United States)

    Fahrenbach, W H; Arango, Claudia P

    2007-11-01

    The digestive system of several species of sea spiders (Pycnogonida, Arthropoda) was studied by electron microscopy. It is composed of the foregut inside a long proboscis, a midgut and a hindgut. Lips near the three jaws at the tip of the proboscis receive several hundred ductules originating from salivary glands. These previously undetected glands open on the lips, a fluted, projecting ridge at the external hinge line of the jaws, i.e., to the outside of the mouth. This disposition suggests affinities to the chelicerate line. The trigonal esophagus within the proboscis contains a complex, setose filter device, operated by dedicated muscles, that serves to reduce ingested food to subcellular dimensions. The midgut has diverticula into the bases of all legs. Its cells differentiate from the basal layer and contain a bewildering array of secretion droplets, lysosomes and phagosomes. In the absence of a hepatopancreas, the midgut serves both digestive and absorptive functions. The cuticle-lined hindgut lies in the highly reduced, peg-like abdomen. Traditionally, pycnogonids have been claimed to have no excretory organ at all. Such a structure, however, has been located in at least one ammotheid, Nymphopsis spinosissima, in which a simple, but standard, excretory gland has been found in the scape of the chelifore. It consists of an end sac, a straight proximal tubule, a short distal tubule, and a raised nephropore. The end sac is a thin-walled and polygonal chamber, about 150 microm in cross section, suspended in the hemocoel of the appendage, its edges radially tethered to the cuticle at more than half a dozen locations. This wall consists of a filtration basement membrane, 1-4 microm thick, facing the hemocoel, and internally of a continuous carpet of podocytes and their pedicels. The podocytes, measuring maximally 10 by 15 microm, have complex contents, of which a labyrinthine system of connected intracellular channels stands out. These coated cisternae open into a

  17. Intelligent control of HVAC systems. Part II: perceptron performance analysis

    Directory of Open Access Journals (Sweden)

    Ioan URSU

    2013-09-01

    Full Text Available This is the second part of a paper on intelligent type control of Heating, Ventilating, and Air-Conditioning (HVAC systems. The whole study proposes a unified approach in the design of intelligent control for such systems, to ensure high energy efficiency and air quality improving. In the first part of the study it is considered as benchmark system a single thermal space HVAC system, for which it is assigned a mathematical model of the controlled system and a mathematical model(algorithm of intelligent control synthesis. The conception of the intelligent control is of switching type, between a simple neural network, a perceptron, which aims to decrease (optimize a cost index,and a fuzzy logic component, having supervisory antisaturating role for neuro-control. Based on numerical simulations, this Part II focuses on the analysis of system operation in the presence only ofthe neural control component. Working of the entire neuro-fuzzy system will be reported in a third part of the study.

  18. Test runs of a Belle II PXD prototype readout system

    Energy Technology Data Exchange (ETDEWEB)

    Getzkow, Dennis; Kuehn, Wolfgang; Lange, Soeren; Lautenbach, Klemens [Justus-Liebig-Universitaet Giessen, II. Physikalisches Institut (Germany); Gessler, Thomas [KEK, Tsukuba (Japan); Collaboration: Belle II-Collaboration

    2016-07-01

    The Belle II PXD readout system (called ONSEN for Online Selection Nodes) uses ATCA (Advanced Telecommunications Architecture) boards with Xilinx Virtex-5 FX70T FPGAs and high speed optical links (6.5 Gbit/s each). The full system consists of 9 carrier boards and 33 daughter cards. The ONSEN system has several interfaces: (a) it receives PXD data from the DHH (Data Handling Hybrid) system, (b) it receives ROI (Regions-of-Interest) data for online data reduction from the HLT (High Level Trigger) system by GbE, and (c) it features data ports to two event builders: EVB1 combines data from all detectors except PXD (in order to generate the ROIs) and EVB2 combines the reduced PXD data with all other data. One of the critical issues is the matching of trigger numbers in the data (received by DHH from the timing distribution system) and trigger numbers in the ROIs (received by the HLT). In order to test the interfaces, in particular for a high HLT rate up to 30 kHz, a prototype system with 3 daughter cards was installed at KEK and tested with DHH, HLT and EVB2. Test results are presented.

  19. The Argus(®) II Retinal Prosthesis System.

    Science.gov (United States)

    Luo, Yvonne Hsu-Lin; da Cruz, Lyndon

    2016-01-01

    The Argus(®) II Retinal Prosthesis System (Second Sight Medical Products) is the first prosthetic vision device to obtain regulatory approval in both Europe and the USA. As such it has entered the commercial market as a treatment for patients with profound vision loss from end-stage outer retinal disease, predominantly retinitis pigmentosa. To date, over 100 devices have been implanted worldwide, representing the largest group of patients currently treated with visual prostheses. The system works by direct stimulation of the relatively preserved inner retina via epiretinal microelectrodes, thereby replacing the function of the degenerated photoreceptors. Visual information from a glasses-mounted video camera is converted to a pixelated image by an external processor, before being transmitted to the microelectrode array at the macula. Elicited retinal responses are then relayed via the normal optic nerve to the cortex for interpretation. We reviewed the animal and human studies that led to the development of the Argus(®) II device. A sufficiently robust safety profile was demonstrated in the phase I/II clinical trial of 30 patients. Improvement of function in terms of orientation and mobility, target localisation, shape and object recognition, and reading of letters and short unrehearsed words have also been shown. There remains a wide variability in the functional outcomes amongst the patients and the factors contributing to these performance differences are still unclear. Future developments in terms of both software and hardware aimed at improving visual function have been proposed. Further experience in clinical outcomes is being acquired due to increasing implantation.

  20. The readout system of the MAGIC-II Cherenkov Telescope

    CERN Document Server

    Tescaro, D; Barcelo, M; Bitossi, M; Cortina, J; Fras, M; Hadasch, D; Illa, J M; Martínez, M; Mazin, D; Paoletti, R; Pegna, R

    2009-01-01

    In this contribution we describe the hardware, firmware and software components of the readout system of the MAGIC-II Cherenkov telescope on the Canary island La Palma. The PMT analog signals are transmitted by means of optical fibers from the MAGIC-II camera to the 80 m away counting house where they are routed to the new high bandwidth and fully programmable receiver boards (MONSTER), which convert back the signals from optical to electrical ones. Then the signals are split, one half provide the input signals for the level ONE trigger system while the other half is sent to the digitizing units. The fast Cherenkov pulses are sampled by low-power Domino Ring Sampler chips (DRS2) and temporarily stored in an array of 1024 capacitors. Signals are sampled at the ultra-fast speed of 2 GSample/s, which allows a very precise measurement of the signal arrival times in all pixels. They are then digitized with 12-bit resolution by an external ADC readout at 40 MHz speed. The Domino samplers are integrated in the newly...

  1. Streamlined protein expression and purification using cleavable self-aggregating tags

    Directory of Open Access Journals (Sweden)

    Zhou Bihong

    2011-06-01

    Full Text Available Abstract Background Recombinant protein expression and purification remains a fundamental issue for biotechnology. Recently we found that two short self-assembling amphipathic peptides 18A (EWLKAFYEKVLEKLKELF and ELK16 (LELELKLKLELELKLK can induce the formation of active protein aggregates in Escherichia coli (E. coli, in which the target proteins retain high enzymatic activities. Here we further explore this finding to develop a novel, facile, matrix-free protein expression and purification approach. Results In this paper, we describe a streamlined protein expression and purification approach by using cleavable self-aggregating tags comprising of one amphipathic peptide (18A or ELK16 and an intein molecule. In such a scheme, a target protein is first expressed as active protein aggregate, separated by simple centrifugation, and then released into solution by intein-mediated cleavage. Three target proteins including lipase A, amadoriase II and β-xylosidase were used to demonstrate the feasibility of this approach. All the target proteins released after cleavage were highly active and pure (over 90% in the case of intein-ELK16 fusions. The yields were in the range of 1.6-10.4 μg/mg wet cell pellet at small laboratory scale, which is comparable with the typical yields from the classical his-tag purification, the IMPACT-CN system (New England Biolabs, Beverly, MA, and the ELP tag purification scheme. Conclusions This tested single step purification is capable of producing proteins with high quantity and purity. It can greatly reduce the cost and time, and thus provides application potentials for both industrial scale up and laboratorial usage.

  2. Millions of Single Cloud Weak MgII Systems

    CERN Document Server

    Churchill, C W; Charlton, J; Churchill, Chris; Rigby, Jane; Charlton, Jane

    2001-01-01

    We report on a population of absorption systems selected by the presence of very weak Mg II doublets. A sub-population of these systems are iron enriched and have near solar metallicities. This would indicated advanced stages (i.e. few Gyr) of in situ star formation within the absorbing structures. From photoionization modeling, we infer low ionization fractions of f(HI/H)~0.01, and gas densities of ~0.1 cm^-3. Since the maximum HI column densities are \\~10^17 cm^-2, the inferred cloud sizes are ~10 pc. From their redshift number densities, this implies that their co-moving spatial density outnumbers normal bright galaxies by a factor of a few million.

  3. Selective expression of latency-associated peptide (LAP) and IL-1 receptor type I/II (CD121a/CD121b) on activated human FOXP3+ regulatory T cells allows for their purification from expansion cultures

    Science.gov (United States)

    Andersson, John; Hardwick, Donna; Bebris, Lolita; Illei, Gabor G.

    2009-01-01

    Although adoptive transfer of regulatory T cells (Foxp3+ Tregs) has proven to be efficacious in the prevention and treatment of autoimmune diseases and graft-versus-host disease in rodents, a major obstacle for the use of Treg immunotherapy in humans is the difficulty of obtaining a highly purified preparation after ex vivo expansion. We have identified latency-associated peptide (LAP) and IL-1 receptor type I and II (CD121a/CD121b) as unique cell-surface markers that distinguish activated Tregs from activated FOXP3− and FOXP3+ non-Tregs. We show that it is feasible to sort expanded FOXP3+ Tregs from non-Tregs with the use of techniques for magnetic bead cell separation based on expression of these 3 markers. After separation, the final product contains greater than 90% fully functional FOXP3+ Tregs. This novel protocol should facilitate the purification of Tregs for both cell-based therapies as well as detailed studies of human Treg function in health and disease. PMID:19299332

  4. Purification of ß-glucosidases from Pichia etchellsii using CIM monolith columns.

    Science.gov (United States)

    Gaonkar, Roopa K; Mishra, Saroj; Vijayalakshmi, M A

    2011-05-01

    β-Glucosidases (EC 3.2.1.21) are industrially important glycosyl hydrolases used for cellulose saccharification as well as for synthesis of glyco-conjugates. Crystal structure of only one β-glucosidase of family 3 of the glycosyl hydrolase families is available due to difficulty in purification of these closely related enzymes from a given source. Multiple steps used during purification result in low yield, making it difficult to study their properties. Conditions for purification of two closely related β-glucosidases (BGL I and BGL II) of family 3 from Pichia etchellsii were investigated in this study. Two weak anion exchange columns convective interaction media-diethyl amino ethyl (CIM-DEAE) and CIM-ethylenediamine (CIM-EDA) were used for this purpose. The results obtained at 0.34 ml disk (CIM-DEAE) level were scaled up to 8 ml CIM-DEAE tube column wherein BGL I and BGL II were separated from the major contaminants in the cell-free extract. The recovered enzymes were completely resolved in the second step using CIM-EDA. A final specific activity of 9,180 IU/mg and 2,345.3 IU/mg was achieved for BGL I and BGL II respectively with an overall yield of 33%. The system should be applicable to resolution of other closely related enzymes from this family.

  5. Affinity Purification of Protein Complexes Using TAP Tags

    Science.gov (United States)

    Gerace, Erica; Moazed, Danesh

    2016-01-01

    This protocol is used for the isolation and analysis of protein complexes using the tandem affinity purification (TAP) tag system. The protocol describes the purification of a protein fused to a TAP tag comprised of two protein A domains and the calmodulin binding peptide separated by a TEV cleavage site. This is a powerful technique for rapid purification of protein complexes and the analysis of their stoichiometric composition, posttranslational modifications, structure, and functional activities. PMID:26096502

  6. [Studies on the immunodiagnosis of rabbit clonorchiasis II. Immunoaffinity purification of whole worm antigen and characterization of egg, metacercaria and adult antigens of Clonorchis sinensis

    Science.gov (United States)

    Lee, Ok Ran; Chung, Pyung Rim; Nam, Hae Seon

    1988-06-01

    antigenic. However, this antigen might reveal cross reactions with other trematodes such as Paragonimus westermani, therefore, purification of antigenic proteins from the crude antigen is essential to increase the sensitivity and specificity for the immunodiagnosis of clonorchiasis.

  7. 天然气净化厂安全防火防爆系统的应用分析%Application and analysis of the safety and fire protection and explosion protection system of natural gas purification plant

    Institute of Scientific and Technical Information of China (English)

    都永昌

    2016-01-01

    本文就天然气净化厂安全防火防爆系统的应用进行分析,以促进天然气净化厂对安全工作的重视程度,提升安全管理水平。%In this paper, the application of natural gas purification plant safety fire and explosion protection system is analyzed, in order to promote the natural gas purification plant to the safety work of attention, improve the level of safety management.

  8. Efficiencies of different flue gas purification systems in waste incinerators. Experimental report; Untersuchungen zur Effizienz von unterschiedlichen Systemen zur Rauchgasreinigung bei Muellverbrennungsanlagen. Untersuchungsbericht

    Energy Technology Data Exchange (ETDEWEB)

    Berghoff, R. (comp.)

    2001-07-01

    This investigation was carried out on behalf of the Environmental Office of the state of Nordrhein-Westfalen. Apart from the current and projected exhaust purification systems, also three exemplary systems were analyzed from the ecological and economic point of view. Effects on pollutant freights were established in consideration of energy consumption and the specific cost of waste treatment for each technology. [German] Das konkrete Ziel dieser vom Landesumweltamt NRW vergebenen Untersuchung war es, neben einer Uebersicht ueber die zzt. eingesetzten und auch geplanten Abgasreinigungssysteme (RGR) bei Muellverbrennungsanlagen, die Vor- und die Nachteile von 3 beispielhaft ausgewaehlten Systemen in einem oekologischen und oekonomischen Vergleich knapp und begrenzt darstellen zu lassen. Als Ergebnis wurden die Auswirkungen der unterschiedlichen Verfahren auf die Frachten der emittierten Schadstoffe unter Beruecksichtigung der unterschiedlichen Energieverbraeuche ermittelt und dargestellt. Darueber hinaus wurden die spezifischen Muellbehandlungskosten bei den jeweiligen Gasreinigungstechniken errechnet. (orig.)

  9. Design and development of single stage purification of papain using Ionic Liquid based aqueous two phase extraction system and its Partition coefficient studies

    Directory of Open Access Journals (Sweden)

    Senthilkumar Rathnasamy

    2013-04-01

    Full Text Available As an emerging trend in bioseparation, aqueous two phase extractions based on phosponium ionic liquid have been utilized in this work to extract papain from Carica papaya fruit latex and the same wascompared with conventional aqueous two phase extraction system. Factors affecting the partition coefficient of papain such as ionic liquid concentration, pH of the extraction system and temperature have been investigated. The optimization studies show that ionic liquid concentrations and pH are majorly influencing the phaseformations and papain partitioning. It reveals the importance of electrostatic and hydrophobic interactions in the papain partitioning. Purification studies performed on Gel Filtration Chromatography shows that 96% of the papain enzyme could be extracted with the phosponium based ionic liquid in a single stage extraction. The final fraction containing papain enzyme was confirmed by SDS Page analysis.

  10. Absorption heat pump integrated in an effluent purification system; Bomba de calor por absorcion integrada a un sistema de purificacion de efluentes

    Energy Technology Data Exchange (ETDEWEB)

    Santoyo, Socrates; Siqueiros, Javier; Heard, Christopher; Santoyo, Edgar [Instituto de Investigaciones Electricas, Cuernavaca (Mexico)

    1996-12-31

    The results derived of the integration of an absorption heat pump to an industrial effluents purification system, are presented. The advantages of these heat pumps with respect to heat pumps by mechanical compression of vapor, as well as the advantages in using absorption heat pumps in simple distillation systems, are mentioned. Finally, a description is made of the equipment designed and built, as well as the results obtained in a preliminary test. [Espanol] Se presentan los resultados derivados de la integracion de una bomba de calor por absorcion a un sistema de purificacion de efluentes industriales. Se mencionan las ventajas de este tipo de bombas de calor con respecto a las de calor por compresion mecanica de vapor, asi como las ventajas de usar bombas de calor en sistemas de destilacion simple. Finalmente, se describe el equipo disenado y construido, asi como los resultados obtenidos de una prueba preliminar.

  11. Mg II Absorption Systems in SDSS QSO Spectra

    CERN Document Server

    Nestor, D B; Rao, S M

    2004-01-01

    We present the results of a MgII absorption-line survey using QSO spectra from the SDSS EDR. Over 1,300 doublets with rest equivalent widths greater than 0.3\\AA and redshifts $0.366 \\le z \\le 2.269$ were identified and measured. We find that the $\\lambda2796$ rest equivalent width ($W_0^{\\lambda2796}$) distribution is described very well by an exponential function $\\partial N/\\partial W_0^{\\lambda2796} = \\frac{N^*}{W^*} e^{-\\frac{W_0}{W^*}}$, with $N^*=1.187\\pm0.052$ and $W^*=0.702\\pm0.017$\\AA. Previously reported power law fits drastically over-predict the number of strong lines. Extrapolating our exponential fit under-predicts the number of $W_0 \\le 0.3$\\AA systems, indicating a transition in $dN/dW_0$ near $W_0 \\simeq 0.3$\\AA. A combination of two exponentials reproduces the observed distribution well, suggesting that MgII absorbers are the superposition of at least two physically distinct populations of absorbing clouds. We also derive a new redshift parameterization for the number density of $W_0^{\\lambd...

  12. Blade System Design Study. Part II, final project report (GEC).

    Energy Technology Data Exchange (ETDEWEB)

    Griffin, Dayton A. (DNV Global Energy Concepts Inc., Seattle, WA)

    2009-05-01

    As part of the U.S. Department of Energy's Low Wind Speed Turbine program, Global Energy Concepts LLC (GEC)1 has studied alternative composite materials for wind turbine blades in the multi-megawatt size range. This work in one of the Blade System Design Studies (BSDS) funded through Sandia National Laboratories. The BSDS program was conducted in two phases. In the Part I BSDS, GEC assessed candidate innovations in composite materials, manufacturing processes, and structural configurations. GEC also made recommendations for testing composite coupons, details, assemblies, and blade substructures to be carried out in the Part II study (BSDS-II). The BSDS-II contract period began in May 2003, and testing was initiated in June 2004. The current report summarizes the results from the BSDS-II test program. Composite materials evaluated include carbon fiber in both pre-impregnated and vacuum-assisted resin transfer molding (VARTM) forms. Initial thin-coupon static testing included a wide range of parameters, including variation in manufacturer, fiber tow size, fabric architecture, and resin type. A smaller set of these materials and process types was also evaluated in thin-coupon fatigue testing, and in ply-drop and ply-transition panels. The majority of materials used epoxy resin, with vinyl ester (VE) resin also used for selected cases. Late in the project, testing of unidirectional fiberglass was added to provide an updated baseline against which to evaluate the carbon material performance. Numerous unidirectional carbon fabrics were considered for evaluation with VARTM infusion. All but one fabric style considered suffered either from poor infusibility or waviness of fibers combined with poor compaction. The exception was a triaxial carbon-fiberglass fabric produced by SAERTEX. This fabric became the primary choice for infused articles throughout the test program. The generally positive results obtained in this program for the SAERTEX material have led to its

  13. Automated System for Control of the Vacuum Diagnostic System for the TJ-II; Control Automatico de los Sistemas de Vacio de Diagnosticos del Dispositivos TJ-II

    Energy Technology Data Exchange (ETDEWEB)

    Lopez Sanchez, A.; Montoro Peinado, A.; Encabo Fernandez, J.; Gama de la Serrano, J.; Sanchez Sarabia, E. [Ciemat, Madrid (Spain)

    2000-07-01

    This report describes the monitorization and remote control systems belonging to the high vacuum systems of the TJ-II diagnostics. These systems are part of each diagnostic and their control has been integrated into the automata that carries out this task. All the controllers are connected through a Profibus network, so as to interchange data between themselves as well as between the general system of TJ-II. (Author)

  14. The Home Air Quality Purification System Based on Internet of Things%基于物联网的家居空气质量净化系统

    Institute of Scientific and Technical Information of China (English)

    蔡文郁; 徐建军; 王洪亮

    2016-01-01

    For the current status of air pollution and the demand for healthy indoor environment,the paper studies a type of home purification system which based on the internet of things (IoT).The system uses the ARM-Cortex-M0 core micro-controller NuMicro-M058 as the hardware platform,and RTX embedded operating system as the software platform,with three filters,a variety of purification photo catalytic disinfection,ultraviolet disinfection,etc.The air purifier also has a collection function for air quality data such as PM2.5,VOC,temperature and humidity.It also can connect through the server and mobile client applications to implement remote monitoring.%针对目前大气污染的现状和人们对健康室内环境的需求,研究并设计了一种基于物联网的家居空气质量净化系统.系统以 ARM-Cortex-M0内核微控制器 NuMicro-M058芯片为硬件核心,采用嵌入式 RTX 操作系统为软件平台,具有三层滤网、光触媒消毒、紫外线杀菌等多种净化功能,而且还具有 PM 2.5,VOC,温湿度等空气质量数据采集功能,最终通过服务器与手机端程序的编写实现了物联网化远程监控.

  15. Development of Diesel Exhaust Aftertreatment System for Tier II Emissions

    Energy Technology Data Exchange (ETDEWEB)

    Yu, R. C.; Cole, A. S., Stroia, B. J.; Huang, S. C. (Cummins, Inc.); Howden, Kenneth C.; Chalk, Steven (U.S. Dept. of Energy)

    2002-06-01

    system design and analysis, critical lab/engine experiments, and ranking then selection of NOX control technologies against reliability, up-front cost, fuel economy, service interval/serviceability, and size/weight. The results of the investigations indicate that the best NOX control approach for LDV and LDT applications is a NOX adsorber system. A greater than 83% NOX reduction efficiency is required to achieve 0.07g/mile NOX Tier II vehicle-out emissions. Both active lean NOX and PACR technology are currently not capable of achieving the high conversion efficiency required for Tier II, Bin 5 emissions standards. In this paper, the NOX technology assessment and selection is first reviewed and discussed. Development of the selected NOX technology (NOX adsorber) and PM control are then discussed in more detail. Discussion includes exhaust sulfur management, further adsorber formulation development, reductant screening, diesel particulate filter development & active regeneration, and preliminary test results on the selected integrated SOX trap, NOX adsorber, and diesel particulate filter system over an FTP-75 emissions cycle, and its impact on fuel economy. Finally, the direction of future work for continued advanced aftertreatment technology development is discussed. (SAE Paper SAE-2002-01-1867 © 2002 SAE International. This paper is published on this website with permission from SAE International. As a user of this website, you are permitted to view this paper on-line, download this pdf file and print one copy of this paper at no cost for your use only. The downloaded pdf file and printout of this SAE paper may not be copied, distributed or forwarded to others or for the use of others.)

  16. Sewage Purification Business Process Management

    Directory of Open Access Journals (Sweden)

    Esad Ahmetagić

    2011-09-01

    Full Text Available This paper presents the current level of drainage and sewage purification facilities built in the Autonomous Province of Vojvodina, a territorial unit of the Republic of Serbia. It also points out the issues related to organized business management in companies involved in this business.The management of business processes in sewage purification involves a comprehensive cycle: business organizing process, issues of standard, investments, workforce, and information system design as factors in establishing an effective organization of business processes. The definition of gap existing between the current approach to organizing business activities and the need to establish an approach based on knowledge, information technologies, and effective business process management points to the necessity for organization redesign and standard definition in business process management. Sewage purification business process management in Vojvodina, the Republic of Serbia has been elaborated through theoretical presentation and a practical example realized by electronic ISO 9001:2008 system of quality management in public water utility company JKP "Vodokanal" Sombor.

  17. 组合吸收塔在 VCM 净化系统中的应用%Application of combined absorption tower in VCM purification system

    Institute of Scientific and Technical Information of China (English)

    田长波; 邵海珑; 齐念先; 赵辉旭

    2013-01-01

    The reform of VCM purification system was performed .The falling film-water washing process was replaced by combined absorption tower process .After the reform ,the absorp-tion efficiency increased ,the water consumption and the caustic soda consumption decreased ,the device life prolonged ,and the cost was saved by 1 .464 4 million RMB ¥ per year .%  对 VCM 净化系统进行了改造,采用组合吸收塔工艺替代降膜水洗吸收工艺。改造后系统的吸收效率提高,水消耗量和烧碱用量减少,设备使用寿命延长,可节约成本146.44万元/a 。

  18. PURIFICATION AND ANALYSIS OF CARBOHYDRATE CLUSTER FROM ERYTHROPOIETIN RECOMBINANT EXPRESSION RESULT ON THE LEAVEN SYSTEM OF Pichia pastoris

    Directory of Open Access Journals (Sweden)

    Adi Santoso

    2011-04-01

    Full Text Available For clinical purposes, pure protein and identification of carbohydrate structure from erythropoietin recombinant are needed. Purification was done with His-Trap affinity chromatography method and continued with gel filtration chromatography column to get purer protein. The carbohydrate cluster from the resulting pure protein then can be recognized by using N- and O-glycosidase and can be compared to EPO recombinant from mammal cells. The result showed similarity on the declining trend of the protein molecule’s weight, which could be seen using the Western blot method. Pure oligosaccharide was hydrolyzed to produce various monosaccharide through incubation with HCl 4 N in 100 oC temperature for 6 hours and the result was applied on high intensity liquid chromatography incubator to learn the composition of its monosaccharide.

  19. Purification and partial biochemical characterization of a membrane-bound type II-like α-glucosidase from the yeast morphotype of Sporothrix schenckii.

    Science.gov (United States)

    Torres-Rodríguez, Blanca I; Flores-Berrout, Karina; Villagómez-Castro, Julio C; López-Romero, Everardo

    2012-02-01

    The early steps of glycoprotein biosynthesis involve processing of the N-glycan core by endoplasmic reticulum α-glucosidases I and II which sequentially trim the outermost α1,2-linked and the two more internal α1,3-linked glucose units, respectively. We have demonstrated the presence of some components of the enzymic machinery required for glycoprotein synthesis in Sporothrix schenckii, the etiological agent of human and animal sporotrichosis. However, information on this process is still very limited. Here, a distribution analysis of α-glucosidase revealed that 38 and 50% of total enzyme activity were present in a soluble and in a mixed membrane fraction, respectively. From the latter, the enzyme was solubilized, purified to apparent homogeneity and biochemically characterized. Analysis of the enzyme by denaturing electrophoresis and size exclusion chromatography revealed molecular masses of 75.4 and 152.7 kDa, respectively, suggesting a homodimeric structure. Purified α-glucosidase cleaved the fluorogenic substrate 4-methylumbelliferyl-α-D: -glucopyranoside with high affinity as judged from K(m) and V(max) values of 0.3 μM and 250 nmol of MU/min/mg protein, respectively. Analysis of linkage specificity using a number of glucose α-disaccharides as substrates demonstrated a clear preference of the enzyme for nigerose, an α1,3-linked disaccharide, over other substrates such as kojibiose (α1,2), trehalose (α1,1) and isomaltose (α1,6). Use of selective inhibitors of processing α-glucosidases such as 1-deoxynojirimycin, castanospermine and australine provided further evidence of the possible type of α-glucosidase. Accordingly, 1-deoxynojirimycin, a more specific inhibitor of α-glucosidase II than I, was a stronger inhibitor of hydrolysis of 4-methylumbelliferyl-α-D: -glucopyranoside and nigerose than castanospermine, a preferential inhibitor of α-glucosidase I. Inhibition of hydrolysis of kojibiose and maltose by 1-deoxynojirimycin and

  20. Gas purification process

    Energy Technology Data Exchange (ETDEWEB)

    Hoelter, H.; Gresch, H.; Igelbuescher, H.; Dewert, H.

    1987-08-13

    To avoid the problems of reheating in a wet process as well as the problems of higher gas supply in a dry process, the invention proposes to separate the raw gas in two component currents, one of which undergoes wet purification while the other is led through a dry purification process. The two component currents are mixed before entering the stack. The dry chemisorption masses added in substoichiometric doses are treated in a milk-of-lime processing stage, after which the reacted and non-reacted chemisorption masses are treated by wet purification and then by oxidation.

  1. Angiotensin II dependent cardiac remodeling in the eel Anguilla anguilla involves the NOS/NO system

    DEFF Research Database (Denmark)

    Filice, Mariacristina; Amelio, Daniela; Garofalo, Filippo

    2017-01-01

    Angiotensin II (AngII), the principal effector of the Renin-Angiotensin System (RAS), plays an important role in controlling mammalian cardiac morpho-functional remodelling. In the eel Anguilla anguilla, one month administration of AngII improves cardiac performance and influences the expression...

  2. Nonterminals, homomorphisms and codings in different variations of OL-systems. II. Nondeterministic systems

    DEFF Research Database (Denmark)

    Nielsen, Mogens; Rozenberg, Grzegorz; Salomaa, Arto

    1974-01-01

    Continuing the work begun in Part I of this paper, we consider now variations of nondeterministic OL-systems. The present Part II of the paper contains a systematic classification of the effect of nonterminals, codings, weak codings, nonerasing homomorphisms and homomorphisms for all basic variat...

  3. Inducing magnetic communication in caged dinuclear Co(II) systems.

    Science.gov (United States)

    Caballero-Jiménez, Judith; Habib, Fatemah; Ramírez-Rosales, Daniel; Grande-Aztatzi, Rafael; Merino, Gabriel; Korobkov, Ilia; Singh, Mukesh Kumar; Rajaraman, Gopalan; Reyes-Ortega, Yasmi; Murugesu, Muralee

    2015-05-14

    The synthesis, structural, electronic and magnetic characterization of five dinuclear Co(II) azacryptand compounds (1-5) bridged through different ions are reported. The magnetic exchange interactions, 2J values, obtained from theoretical computations show that the variation of the intermetallic angles and distances lead to antiferromagnetic behaviours. Magneto-structural correlations show a trend, where the angles Co(II)-bridge-Co(II) closer to 180° favour an increase in the superexchange pathway leading to higher AF interaction values.

  4. Expert system applications in support of system diagnostics and prognostics at EBR-II

    Energy Technology Data Exchange (ETDEWEB)

    Lehto, W.K.; Gross, K.C. (Argonne National Lab., Idaho Falls, ID (USA); Argonne National Lab., IL (USA))

    1989-01-01

    Expert systems have been developed to aid in the monitoring and diagnostics of the Experimental Breeder Reactor-II (EBR-II) at the Idaho National Engineering Laboratory (INEL) in Idaho Falls, Idaho. Systems have been developed for failed fuel surveillance and diagnostics and reactor coolant pump monitoring and diagnostics. A third project is being done jointly by ANL-W and EG G Idaho to develop a transient analysis system to enhance overall plant diagnostic and prognostic capability. The failed fuel surveillance and diagnosis system monitors, processes, and interprets information from nine key plant sensors. It displays to the reactor operator diagnostic information needed to make proper decisions regarding technical specification conformance during reactor operation with failed fuel. 8 refs., 9 figs., 2 tabs.

  5. Overview of the purification of recombinant proteins.

    Science.gov (United States)

    Wingfield, Paul T

    2015-04-01

    When the first version of this unit was written in 1995, protein purification of recombinant proteins was based on a variety of standard chromatographic methods and approaches, many of which were described and mentioned throughout Current Protocols in Protein Science. In the interim, there has been a shift toward an almost universal usage of the affinity or fusion tag. This may not be the case for biotechnology manufacture where affinity tags can complicate producing proteins under regulatory conditions. Regardless of the protein expression system, questions are asked as to which and how many affinity tags to use, where to attach them in the protein, and whether to engineer a self-cleavage system or simply leave them on. We will briefly address some of these issues. Also, although this overview focuses on E.coli, protein expression and purification, other commonly used expression systems are mentioned and, apart from cell-breakage methods, protein purification methods and strategies are essentially the same.

  6. One-step purification of E. coli elongation factor Tu

    DEFF Research Database (Denmark)

    Knudsen, Charlotte Rohde; Clark, Brian F. C.; Degn, B

    1993-01-01

    The tuf A gene, encoding the E. coli elongation factor Tu, was cloned in the pGEX gene fusion system. Upon expression EF-Tu is fused to glutathione-S-transferase serving as a purification handle with affinity for glutathione immobilised on agarose. This allows purification of EF-Tu in a one...

  7. One-step purification of E. coli elongation factor Tu

    DEFF Research Database (Denmark)

    Knudsen, Charlotte Rohde; Clark, Brian F. C.; Degn, B

    1993-01-01

    The tuf A gene, encoding the E. coli elongation factor Tu, was cloned in the pGEX gene fusion system. Upon expression EF-Tu is fused to glutathione-S-transferase serving as a purification handle with affinity for glutathione immobilised on agarose. This allows purification of EF-Tu in a one...

  8. Cost effective purification of intein based syntetic cationic antimicrobial peptide expressed in cold shock expression system using salt inducible E. coli GJ1158

    Directory of Open Access Journals (Sweden)

    Seetha Ram Kotra

    2014-03-01

    Full Text Available Objective:Synthetic cationic antimicrobial peptide (SC-AMP is an important and upcoming therapeutic molecule against onventional antibiotics. In this study, an attempt was made to purify the SC-AMP without the enzymatic cleavage of the affinity tag, by using an intein-based system. Methods:The intein sequence was amplified from pTYB11 vector using PCR methodologies and the N-terminal of intein was ligated with SC-AMP. The designed construct, intein-SC-AMP was cloned into MCS region of cold shock expression vector, pCOLDI and the recombinant peptide was purified on a chitin affinity column by cleaving intein with 50 mM DTT without applying enzymatic cleavage. Later the peptide was quantified and its antibacterial activity of the purified peptide was studied using well diffusion method. Results: Initially, intein-SC-AMP was expressed as a fusion protein in both IPTG inducible E. coli BL21(DE3 and salt inducible E. coli GJ1158. Single step purification using CBD (chitin binding domain - intein tag in salt inducible E. coli GJ1158, yields the SC-AMP in the soluble form at a oncentration of 208 mg/L. The antibacterial activity and minimal inhibitory concentration (MIC of the purified SC-AMP was studied against both Gram positive and Gram negative microorganisms. Conclusion: For the first time, single step purification of soluble SC-AMP was carried out using chitin-binding domain affinity tag in salt inducible E. coli GJ1158 without an application of enzymatic cleavage. J Microbiol Infect Dis 2014;4(1:13-19

  9. Purification of yeast alcohol dehydrogenase by using immobilized metal affinity cryogels

    Energy Technology Data Exchange (ETDEWEB)

    Akduman, Begüm [Chemistry Department, Adnan Menderes University, Aydın (Turkey); Uygun, Murat [Koçarlı Vocational and Training School, Adnan Menderes University, Aydın (Turkey); Uygun, Deniz Aktaş, E-mail: daktas@adu.edu.tr [Chemistry Department, Adnan Menderes University, Aydın (Turkey); Akgöl, Sinan [Biochemistry Department, Ege University, İzmir (Turkey); Denizli, Adil [Chemistry Department, Hacettepe University, Ankara (Turkey)

    2013-12-01

    In this study, poly(2-hydroxyethyl methacrylate–glycidylmethacrylate) [poly(HEMA–GMA)] cryogels were prepared by radical cryocopolymerization of HEMA with GMA as a functional comonomer and N,N′-methylene-bisacrylamide (MBAAm) as a crosslinker. Iminodiacetic acid (IDA) functional groups were attached via ring opening of the epoxy group on the poly(HEMA–GMA) cryogels and then Zn(II) ions were chelated with these structures. Characterization of cryogels was performed by FTIR, SEM, EDX and swelling studies. These cryogels have interconnected pores of 30–50 μm size. The equilibrium swelling degree of Zn(II) chelated poly(HEMA–GMA)-IDA cryogels was approximately 600%. Zn(II) chelated poly(HEMA–GMA)-IDA cryogels were used in the adsorption of alcohol dehydrogenase from aqueous solutions and adsorption was performed in continuous system. The effects of pH, alcohol dehydrogenase concentration, temperature, and flow rate on adsorption were investigated. The maximum amount of alcohol dehydrogenase adsorption was determined to be 9.94 mg/g cryogel at 1.0 mg/mL alcohol dehydrogenase concentration and in acetate buffer at pH 5.0 with a flow rate of 0.5 mL/min. Desorption of adsorbed alcohol dehydrogenase was carried out by using 1.0 M NaCI at pH 8.0 phosphate buffer and desorption yield was found to be 93.5%. Additionally, these cryogels were used for purification of alcohol dehydrogenase from yeast with a single-step. The purity of desorbed alcohol dehydrogenase was shown by silver-stained SDS–PAGE. This purification process can successfully be used for the purification of alcohol dehydrogenase from unclarified yeast homogenates and this work is the first report about the usage of the cryogels for purification of alcohol dehydrogenase. - Highlights: • Poly(HEMA–GMA) cryogels were synthesized by radical cryocopolymerization technique. • Prepared cryogels were functionalized with IDA, then Zn(II) ions were chelated to the cryogel. • Zn(II) chelated poly

  10. The systems biology of MHC class II antigen presentation

    NARCIS (Netherlands)

    Paul, Petra

    2012-01-01

    Major histocompatibility class II molecules (MHC class II) are one of the key regulators of adaptive immunity because of their specific expression by professional antigen presenting cells (APC). They present peptides derived from endocytosed material to T helper lymphocytes. Consequently, MHC class

  11. Solar Electric Generating System II finite element analysis

    Energy Technology Data Exchange (ETDEWEB)

    Dohner, J.L.; Anderson, J.R.

    1994-04-01

    On June 2, 1992, Landers` earthquake struck the Solar Electric Generating System II, located in Daggett, California. The 30 megawatt power station, operated by the Daggett Leasing Corporation (DLC), suffered substantial damage due to structural failures in the solar farm. These failures consisted of the separation of sliding joints supporting a distribution of parabolic glass mirrors. At separation, the mirrors fell to the ground and broke. It was the desire of the DLC and the Solar Thermal Design Assistance Center (STDAC) of Sandia National Laboratories (SNL) and to redesign these joints so that, in the event of future quakes, costly breakage will be avoided. To accomplish this task, drawings of collector components were developed by the STDAC, from which a detailed finite element computer model of a solar collector was produced. This nonlinear dynamic model, which consisted of over 8,560 degrees of freedom, underwent model reduction to form a low order nonlinear dynamic model containing only 40 degrees of freedom. This model was then used as a design tool to estimate joint dynamics. Using this design tool, joint configurations were modified, and an acceptable joint redesign determined. The results of this analysis showed that the implementation of metal stops welded to support shafts for the purpose of preventing joint separation is a suitable joint redesign. Moreover, it was found that, for quakes of Landers` magnitude, mirror breakage due to enhanced vibration in the trough assembly is unlikely.

  12. The Belle II PXD data acquisition and reduction system

    Energy Technology Data Exchange (ETDEWEB)

    Lange, Soeren; Gessler, Thomas; Kuehn, Wolfgang; Muenchow, David; Spruck, Bjoern [Giessen Univ. (Germany). 2. Physikalisches Inst.; Lin, Haichuan; Liu, Zhen-An; Xu, Hao; Zhao, Jingzhou [IHEP Beijing (China); Collaboration: Belle II Collaboration

    2013-07-01

    The Belle II DEPFET pixel detector (PXD) will deliver high data rates of up to 21.6 Gbytes/s for 3% detector occupancy. Data of this high rate must be buffered for 5 seconds, corresponding to the HLT (High Level Trigger) latency, and then a region-of-interest (ROI) filter is applied to reduce the data rate by a factor of ≥10 by charged track extrapolation from other detectors (SVD, CDC). The PXD readout system is based upon ATCA (Advanced Telecommunications Architecture). The 3rd PCB iteration uses a concept with a xTCA carrier board (with a Virtex-4 FX60 FPGA for ATCA backplane routing) and 4 AMC modules (each with a Xilinx Virtex-5 FX70T FPGA). The FPGA firmware implementation comprises a receiver core for the high speed optical links (≤6.25 Gbps), a buffer management with lookup of ≤270.000 pointers/s, DDR2 memory write (native port interface, ≥1.5 Gbytes/s), Gigabit ethernet (UDP stack in VHDL) and a parallelized ROI selection algorithm. Test results of all the components are presented.

  13. Rooftop PV system. Final technical progress report, Phase II

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-08-01

    Under this four-year PV:BONUS Program, ECD and United Solar are developing and demonstrating two new lightweight flexible building integrated Photovoltaic (BIPV) modules specifically designed as exact replacements for conventional asphalt shingles and standing seam metal roofing. These modules can be economically and aesthetically integrated into new residential and commercial buildings, and address the even larger roofing replacement market. The modules are designed to be installed by roofing contractors without special training which minimizes the installation and balance of system costs. The modules will be fabricated from high-efficiency, multiple-junction a-Si alloy solar cells developed by ECD and United Solar. Under the Phase I Program, which ended in March 1994, we developed two different concept designs for rooftop PV modules: (1) the United Solar overlapping (asphalt shingle replacement) shingle-type modules and (2) the ECD metal roof-type modules. We also developed a plan for fabricating, testing and demonstrating these modules. Candidate demonstration sites for our rooftop PV modules were identified and preliminary engineering designs for these demonstrations were developed; a marketing study plan was also developed. The major objectives of the Phase II Program, which started in June 1994 was (1) to develop, test, and qualify these new rooftop modules; (2) to develop mechanical and electrical engineering specifications for the demonstration projects; and (3) to develop a marketing/commercialization plan.

  14. Photocatalytic materials and technologies for air purification.

    Science.gov (United States)

    Ren, Hangjuan; Koshy, Pramod; Chen, Wen-Fan; Qi, Shaohua; Sorrell, Charles Christopher

    2017-03-05

    Since there is increasing concern for the impact of air quality on human health, the present work surveys the materials and technologies for air purification using photocatalytic materials. The coverage includes (1) current photocatalytic materials for the decomposition of chemical contaminants and disinfection of pathogens present in air and (2) photocatalytic air purification systems that are used currently and under development. The present work focuses on five main themes. First, the mechanisms of photodegradation and photodisinfection are explained. Second, system designs for photocatalytic air purification are surveyed. Third, the photocatalytic materials used for air purification and their characteristics are considered, including both conventional and more recently developed photocatalysts. Fourth, the methods used to fabricate these materials are discussed. Fifth, the most significant coverage is devoted to materials design strategies aimed at improving the performance of photocatalysts for air purification. The review concludes with a brief consideration of promising future directions for materials research in photocatalysis. Copyright © 2016 Elsevier B.V. All rights reserved.

  15. Evaluation on licensability of KNGR system design (II)

    Energy Technology Data Exchange (ETDEWEB)

    Park, G. C.; Seo, K. R. [Seoul National Univ., Seoul (Korea, Republic of); Kim, J. K. [Hanyang Univ., Seoul (Korea, Republic of)] (and others)

    2001-01-15

    The CE methodology of DBA analysis are reviewed. Though UPTF test is different from the KNGR in the geometrical configuration of the down-corner, this was used as a reference to investigate the validity of CEFLASH and COMPERC-II codes in DBA analysis of KNGR. it revealed that CEFLASH is conservative but COMPERC-II for the Refill and Reflood phase is needed for the detailed investigation on ECC bypass, Entrainment, condensation phenomena, CCFL. The direct bypass rate based on the benchmark problem was quantitatively measured. The test model was scaled by the linear scaling methodology, and the accident conditions were the reflood phase at a CB-DEGB LBLOCA. The initial total air flow rate was determined considering the volume scaling factor (1/1000) on criteria of the KNGR design value. The volume rates of ECC water injected: through the DVI nozzles were changed for several case. The direct bypass ratio was about 22 % on the condition of the KNGR scaled-down air volume flow and ECC water velocity at about 1 m/s. The fast neutron fluence at the Reactor Pressure Vessel(RPV) of KNGR designed for 60 years of lifetime was calculated by Monte Carlo simulations and Discrete Ordinates Method for reactor pressure vessel integrity assessment. KNGR core geometry was modeled on a three-dimensional. In the full-scope Monte Carlo method, the maximum fast neutron flux at inner vessel belt line was estimated as 2.738 x 10{sup 10} neutrons/cm{sup 2}{center_dot}see. In the ROCS+MCNP4B calculation, the maximum flux of 2.769 x 10{sup 10} neutrons/ cm{sup 2}{center_dot}see at the RPV was obtained by tallying neutrons crossing the inner surface of the RPV. In ROCS+TORT Calculation, the maximum flux of 3.190 x 10{sup 10} neutrons/cm{sup 2} {center_dot}see was obtained at inner RPV belt line. The lifetime of KNGR was estimated on the basis of conservative end of life fluence limit value of the ABB-CE System 80+. Approximately, 72 Effective Full Power Years (EFPYs), equivalent to 90 calendar

  16. High-Yield Soluble Expression and Simple Purification of the Antimicrobial Peptide OG2 Using the Intein System in Escherichia coli

    Directory of Open Access Journals (Sweden)

    Yong-Gang Xie

    2013-01-01

    Full Text Available OG2 is a modified antimicrobial peptide, that is, derived from the frog peptide Palustrin-OG1. It has high antimicrobial activity and low cytotoxicity, and it is therefore promising as a therapeutic agent. Both prokaryotic (Escherichia coli and eukaryotic (Pichia pastoris production host systems were used to produce OG2 in our previous study; however, it was difficult to achieve high expression yields and efficient purification. In this study, we achieved high-yield OG2 expression using the intein fusion system. The optimized OG2 gene was cloned into the pTWIN1 vector to generate pTWIN-OG2-intein2 (C-terminal fusion vector and pTWIN-intein1-OG2 (N-terminal fusion vector. Nearly 70% of the expressed OG2-intein2 was soluble after the IPTG concentration and induction temperature were decreased, whereas only 42% of the expressed of intein1-OG2 was soluble. Up to 75 mg of OG2-intein2 was obtained from a 1 l culture, and 85% of the protein was cleaved by 100 mM DTT. Intein1-OG2 was less amenable to cleavage due to the inhibition of cleavage by the N-terminal amino acid of OG2. The purified OG2 exhibited strong antimicrobial activity against E. coli K88. The intein system is the best currently available system for the cost-effective production of OG2.

  17. Tracer-Encapsulated Solid Pellet (TESPEL) Injection System for the TJ-II Stellarator

    Energy Technology Data Exchange (ETDEWEB)

    Tamura, N. [National Institute for Fusion Science, Toki, Japan; McCarthy, K. J. [EURATOM-CIEMAT, Madrid, Spain; Hayashi, H. [National Institute for Fusion Science, Toki, Japan; Combs, Stephen Kirk [ORNL; Foust, Charles R [ORNL; Garcia, R. [Laboratory Nacional de Fusion, Madrid, Spain; Panadero, N. [CIEMAT, Laboratory Nacional de Fusion, Spain; Pawelec, E. [Opole University, Poland; Sanchez, J. Hernandez [Laboratory Nacional de Fusion, Madrid, Spain; Navarro, M. [CIEMAT, Laboratory Nacional de Fusion, Spain; Soleto, A. [CIEMAT, Laboratory Nacional de Fusion, Spain

    2016-01-01

    A tracer-encapsulated solid pellet (TESPEL) injection system for the TJ-II stellarator was recently developed. In order to reduce the time and cost for the development, we combined a TESPEL injector provided by National Institute for Fusion Science with an existing TJ-II cryogenic pellet injection system. Consequently, the TESPEL injection into the TJ-II plasma was successfully achieved, which was confirmed by several pellet diagnostics including a normal-incidence spectrometer for monitoring a tracer impurity behavior.

  18. Commissioning and Early Operation for the NSLS-II Booster RF System

    Energy Technology Data Exchange (ETDEWEB)

    Marques, C. [Brookhaven National Lab. (BNL), Upton, NY (United States); Cupolo, J. [Brookhaven National Lab. (BNL), Upton, NY (United States); Davila, P. [Brookhaven National Lab. (BNL), Upton, NY (United States); Gao, F. [Brookhaven National Lab. (BNL), Upton, NY (United States); Goel, A. [Brookhaven National Lab. (BNL), Upton, NY (United States); Holub, B. [Brookhaven National Lab. (BNL), Upton, NY (United States); Kulpin, J. [Brookhaven National Lab. (BNL), Upton, NY (United States); McDonald, K. [Brookhaven National Lab. (BNL), Upton, NY (United States); Oliva, J. [Brookhaven National Lab. (BNL), Upton, NY (United States); Papu, J. [Brookhaven National Lab. (BNL), Upton, NY (United States); Ramirez, G. [Brookhaven National Lab. (BNL), Upton, NY (United States); Rose, J. [Brookhaven National Lab. (BNL), Upton, NY (United States); Sikora, R. [Brookhaven National Lab. (BNL), Upton, NY (United States); Sorrentino, C. [Brookhaven National Lab. (BNL), Upton, NY (United States); Towne, N. [Brookhaven National Lab. (BNL), Upton, NY (United States)

    2015-05-03

    The National Synchrotron Light Source II (NSLS-II) at Brookhaven National Laboratory (BNL) is a third generation 3GeV, 500mA synchrotron light source. We discuss the booster synchrotron RF system responsible for providing power to accelerate an electron beam from 200MeV to 3GeV. The RF system design and construction are complete and is currently in the operational phase of the NSLS-II project. Preliminary operational data is also discussed.

  19. Accurate GPS Time-Linked data Acquisition System (ATLAS II) user's manual.

    Energy Technology Data Exchange (ETDEWEB)

    Jones, Perry L.; Zayas, Jose R.; Ortiz-Moyet, Juan (PrimeCore Systems Inc., Albuquerque, NM)

    2004-02-01

    The Accurate Time-Linked data Acquisition System (ATLAS II) is a small, lightweight, time-synchronized, robust data acquisition system that is capable of acquiring simultaneous long-term time-series data from both a wind turbine rotor and ground-based instrumentation. This document is a user's manual for the ATLAS II hardware and software. It describes the hardware and software components of ATLAS II, and explains how to install and execute the software.

  20. SFC在分子筛纯化系统切换控制中的应用%Application of SFC in switching-over control of molecular sieve purification system

    Institute of Scientific and Technical Information of China (English)

    付定君; 伍晓娟; 徐平林

    2011-01-01

    针对分子筛纯化系统切换控制的特点,结合横河CETUMCS3000控制系统中的顺序控制功能,介绍了SFC在分子筛纯化系统切换控制中的应用,以及一些组态经验和维护措施。%Application of SFC in switching-over control of molecular sieve purification system is briefed on basis of the features of switching-over control of molecular sieve purification system in combination with function of sequence control of Henghe CETUM CS3000 control system and experiences on configuration and maintenance measures are described.

  1. Evaluation of a prereduced anaerobically sterilized medium (PRAS II) system for identification anaerobic microorganisms.

    Science.gov (United States)

    Beaucage, C M; Onderdonk, A B

    1982-09-01

    A prereduced, anaerobically sterilized system of tubed media (PRAS II; Scott Laboratories, Fiskeville, R.I.) was evaluated for accuracy in the identification of anerobic microorganisms. PRAS II was found to be a rapid and accurate identification system for obligate anaerobes which does not require the use of gas cannula inoculation or incubation in a special anaerobic environment.

  2. Evaluation of a prereduced anaerobically sterilized medium (PRAS II) system for identification anaerobic microorganisms.

    OpenAIRE

    Beaucage, C M; Onderdonk, A B

    1982-01-01

    A prereduced, anaerobically sterilized system of tubed media (PRAS II; Scott Laboratories, Fiskeville, R.I.) was evaluated for accuracy in the identification of anerobic microorganisms. PRAS II was found to be a rapid and accurate identification system for obligate anaerobes which does not require the use of gas cannula inoculation or incubation in a special anaerobic environment.

  3. Design of the NSLS-II Top-Off Safety System

    Energy Technology Data Exchange (ETDEWEB)

    Fliller, III R. [Brookhaven National Lab. (BNL), Upton, NY (United States). National Synchrotron Light Source; Doom, L. [Brookhaven National Lab. (BNL), Upton, NY (United States). National Synchrotron Light Source; Ganetis, G. [Brookhaven National Lab. (BNL), Upton, NY (United States). National Synchrotron Light Source; Hetzel, C. [Brookhaven National Lab. (BNL), Upton, NY (United States). National Synchrotron Light Source; Job, P. K. [Brookhaven National Lab. (BNL), Upton, NY (United States). National Synchrotron Light Source; Li, Y. [Brookhaven National Lab. (BNL), Upton, NY (United States). National Synchrotron Light Source; Shaftan, T. [Brookhaven National Lab. (BNL), Upton, NY (United States). National Synchrotron Light Source; Sharma, S. [Brookhaven National Lab. (BNL), Upton, NY (United States). National Synchrotron Light Source; Singh, O. [Brookhaven National Lab. (BNL), Upton, NY (United States). National Synchrotron Light Source; Wang, G. M. [Brookhaven National Lab. (BNL), Upton, NY (United States). National Synchrotron Light Source; Xia, Z. [Brookhaven National Lab. (BNL), Upton, NY (United States). National Synchrotron Light Source

    2015-05-03

    The NSLS-II accelerators finished commissioning in the fall of 2014, with beamline commissioning underway. Part of the design for the NSLS-II is to operate in top off mode. The Top Off Safety System (TOSS) is presently being installed. In this report we discuss the Top Off Safety System design and implementation, along with the necessary tracking results and radiological calculations.

  4. Study on the thermodynamics of the gadolinium-hydrogen binary system (H/Gd = 0.0–2.0) and implications to metallic gadolinium purification

    Energy Technology Data Exchange (ETDEWEB)

    Fu, Kai; Li, Guoling [Beijing National Laboratory of Molecular Sciences, State Key Laboratory of Rare Earth Materials Chemistry and Applications, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871 (China); Department of Materials Physics and Chemistry, University of Science and Technology Beijing, NO. 30, Xueyuan Road, Beijing 100083 (China); Li, Jigang [Beijing National Laboratory of Molecular Sciences, State Key Laboratory of Rare Earth Materials Chemistry and Applications, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871 (China); Liu, Yang [Department of Food Science, College of Arts and Science, Beijing Union University, Beijing, 100101 (China); Tian, Wenhuai, E-mail: wenhuaitian@ustb.edu.cn [Department of Materials Physics and Chemistry, University of Science and Technology Beijing, NO. 30, Xueyuan Road, Beijing 100083 (China); Zheng, Jie [Beijing National Laboratory of Molecular Sciences, State Key Laboratory of Rare Earth Materials Chemistry and Applications, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871 (China); Li, Xingguo, E-mail: xgli@pku.edu.cn [Beijing National Laboratory of Molecular Sciences, State Key Laboratory of Rare Earth Materials Chemistry and Applications, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871 (China)

    2016-07-15

    The thermodynamics of the gadolinium-hydrogen (Gd–H) binary system (H/Gd = 0.0–2.0) between 650 and 900 °C is studied by pressure composition isotherm measurement. Significant H dissolution in Gd is observed, up to H/Gd = 0.34 at 650 °C and 0.55 at 900 °C. The metal-rich phase boundary of nonstoichiometric gadolinium dihydride was found to occur with nominal composition of GdH{sub 1.80} at 650 °C and GdH{sub 1.53} at 900 °C. The results are in fairly good agreement with previous experimental work but with improved accuracy. The binary phase diagram is obtained using the CALPHAD method. The thermodynamic study here assists the understanding on the efficient deoxygenation effect by the H in Gd for Gd purification. - Highlights: • PCI measurements with high accuracy has been carried out. • A first assessment of Gd–H system has been carried out by the CALPHAD method. • A set of self-consistent thermodynamic parameters was derived for this system.

  5. Analysis of the Sherlock II tip location system for inserting peripherally inserted central venous catheters.

    Science.gov (United States)

    Lelkes, Valdis; Kumar, Abhishek; Shukla, Pratik A; Contractor, Sohail; Rutan, Thomas

    2013-01-01

    Peripherally inserted central catheters (PICCs) are frequently placed at the bedside. The purpose of our study was to evaluate the efficacy of the Sherlock II tip location system (Bard Access Systems, Salt Lake City, UT), which offers electromagnetic detection of the PICC tip to assist the operator in guiding the tip to a desired location. We performed a retrospective review of patients who had a bedside PICC using the Sherlock II tip location system. Three hundred seventy-five of 384 patients (97.7%) had the catheter tip positioned appropriately. Our results suggest that the Sherlock II tip location system is an efficacious system for bedside PICC placement.

  6. MPS II: adaptive behavior of patients and impact on the family system.

    Science.gov (United States)

    Needham, Mary; Packman, Wendy; Rappoport, Maxwell; Quinn, Natasha; Cordova, Matthew; Macias, Sandra; Morgan, Cynthia; Packman, Seymour

    2014-06-01

    Mucopolysaccharidosis type II (MPS II), also known as Hunter syndrome, is a chronic and progressive X-linked lysosomal disease that mainly affects males. It occurs in 1 in every 65,000 to 1 in 132,000 births. There are two distinct forms of the disease based on age of onset and clinical course: mild and severe. MPS II affects many organ systems including the nervous, cardiovascular, gastrointestinal and respiratory systems. Complications can include vision problems, progressive hearing loss, thickened and elastic skin, mental impairment, and enlarged liver and spleen. We herein focus on the adaptive behavior of individuals with MPS II, and the impact of MPS II on the family system. Outcomes from the Vineland-II Adaptive Behavior Scales showed that the MPS II patient sample experienced significantly lower functioning in communication, daily living skills, socialization, and motor skills compared to normative data. Patients with severe MPS II were found to have significantly lower adaptive functioning in all domains, as compared to those with mild MPS II. Length of time on ERT had no significant relationship to adaptive functioning. Results from the Peds QL Family Impact Module indicated that families of patients with MPS II experienced a lower overall health-related quality of life and overall lower family functioning (including lower emotional and cognitive functioning) than those with chronic illnesses residing in an inpatient setting.

  7. QT dispersion and prognostication of the outcome in acute cardiotoxicities: A comparison with SAPS II and APACHE II scoring systems.

    Science.gov (United States)

    Hassanian-Moghaddam, Hossein; Amiri, Hassan; Zamani, Nasim; Rahimi, Mitra; Shadnia, Shahin; Taherkhani, Maryam

    2014-06-01

    We aimed to evaluate the efficacy of QT dispersion (QTD) in determining the outcome of the patients poisoned by cardiotoxic medications and toxins. Patients who referred to our emergency department (ED) due to acute toxicity with any cardiotoxic medication or toxin and were admitted to medical toxicology intensive care unit (MTICU) were enrolled into the study. A questionnaire containing the demographic characteristics, vital signs, laboratory tests, electrocardiographic (ECG) parameters of the first ECG taken on MTICU or ED admission, simplified acute physiology score (SAPS), and acute physiology and chronic health evaluation (APACHE) score was filled for every single patient. QTD was manually calculated. The patients were divided into two groups of survivors and non-survivors and compared. Although QTD was not significantly different between the survivors and non-survivors (P = 0.8), SAPS II and APACHE II score were so. SAPS and APACHE had the highest sensitivity and specificity in determining the patients' mortality, respectively. SAPS had the highest sensitivity, and QTD had the highest specificity in predicting the later development of the complications. SAPS II and APACHE II scoring systems are the best systems for prognostication of death in patients with acute cardiotoxic medication-induced poisonings. QTD can be successfully used for the prediction of complications.

  8. Application of Moving Bed Biofilm Reactor (MBBR) and Integrated Fixed Activated Sludge (IFAS) for Biological River Water Purification System: A Short Review

    Science.gov (United States)

    Lariyah, M. S.; Mohiyaden, H. A.; Hayder, G.; Hayder, G.; Hussein, A.; Basri, H.; Sabri, A. F.; Noh, MN

    2016-03-01

    This review paper present the MBBR and IFAS technology for urban river water purification including both conventional methods and new emerging technologies. The aim of this paper is to present the MBBR and IFAS technology as an alternative and successful method for treating different kinds of effluents under different condition. There are still current treatment technologies being researched and the outcomes maybe available in a while. The review also includes many relevant researches carried out at the laboratory and pilot scales. This review covers the important processes on MBBR and IFAS basic treatment process, affecting of carrier type and influent types. However, the research concluded so far are compiled herein and reported for the first time to acquire a better perspective and insight on the subject with a view of meeting the news approach. The research concluded so far are compiled herein and reported for the first time to acquire a better perspective and insight on the subject with a view of meeting the news approach. To this end, the most feasible technology could be the combination of advanced biological process (bioreactor systems) including MBBR and IFAS system.

  9. Separation and purification of isorhamnetin 3-sulphate from Flaveria bidentis (L.) Kuntze by counter-current chromatography comparing two kinds of solvent systems.

    Science.gov (United States)

    Xie, Qianqian; Yin, Li; Zhang, Guoliang; Wei, Yun

    2012-01-01

    The first preparative separation of a flavonoid sulphate isorhamnetin 3-sulphate from Flaveria bidentis (L.) Kuntze by counter-current chromatography (CCC) was presented. Two kinds of solvent systems were used. A conventional organic/aqueous solvent system n-butanol-ethyl acetate-water (4:1:5, v/v) was used, yielding isorhamnetin 3-sulphate 2.0 mg with a purity of 93.4% from 83 mg of pre-enriched crude extract obtained from 553 mg ethanol extract by macroporous resin. A one-component organic/salt-containing system composed of n-butanol-0.25% sodium chloride aqueous solution (1:1, v/v) was also used, and the LC column packed with macroporous resin has been employed for desalination of the target compound purified from CCC. As a result, 2.1 mg of isorhamnetin 3-sulphate with a purity of over 97% has been isolated from 402 mg of crude extract without pre-enrichment. Compared with the conventional organic/aqueous system, the one-component organic/salt-containing aqueous system was more suitable for the separation of isorhamnetin 3-sulphate, and purer target compound was obtained from the crude extract without pre-enrichment using the new solvent system. The chemical structure was confirmed by ESI-MS and (1)H, (13)C NMR. In summary, our results indicated that CCC using one-component organic/salt-containing aqueous solution is very promising and powerful for high-throughput purification of isorhamnetin 3-sulphate from Flaveria bidentis (L.) Kuntze.

  10. River Protection Project Integrated safety management system phase II verification report, volumes I and II - 8/19/99

    Energy Technology Data Exchange (ETDEWEB)

    SHOOP, D.S.

    1999-09-10

    The Department of Energy policy (DOE P 450.4) is that safety is integrated into all aspects of the management and operations of its facilities. In simple and straightforward terms, the Department will ''Do work safely.'' The purpose of this River Protection Project (RPP) Integrated Safety Management System (ISMS) Phase II Verification was to determine whether ISMS programs and processes are implemented within RFP to accomplish the goal of ''Do work safely.'' The goal of an implemented ISMS is to have a single integrated system that includes Environment, Safety, and Health (ES&H) requirements in the work planning and execution processes to ensure the protection of the worker, public, environment, and federal property over the RPP life cycle. The ISMS is comprised of the (1) described functions, components, processes, and interfaces (system map or blueprint) and (2) personnel who are executing those assigned roles and responsibilities to manage and control the ISMS. Therefore, this review evaluated both the ''paper'' and ''people'' aspects of the ISMS to ensure that the system is implemented within RPP. Richland Operations Office (RL) conducted an ISMS Phase I Verification of the TWRS from September 28-October 9, 1998. The resulting verification report recommended that TWRS-RL and the contractor proceed with Phase II of ISMS verification given that the concerns identified from the Phase I verification review are incorporated into the Phase II implementation plan.

  11. Enterovirus 71 Virus Propagation and Purification

    OpenAIRE

    Kristin L Shingler; Organtini, Lindsey J.; Hafenstein, Susan

    2014-01-01

    Since its discovery in 1969, enterovirus 71 (EV71) has emerged as a serious worldwide health threat. This member of the picornavirus family causes hand, foot, and mouth disease, and also has the capacity to invade the central nervous system to cause severe disease and death. This is the propagation and purification procedure to produce infectious virion.

  12. Crystallization using reverse micelles and water-in-oil microemulsion systems: the highly selective tool for the purification of organic compounds from complex mixtures.

    Science.gov (United States)

    Kljajic, Alen; Bester-Rogac, Marija; Klobcar, Andrej; Zupet, Rok; Pejovnik, Stane

    2013-02-01

    The active pharmaceutical ingredient orlistat is usually manufactured using a semi-synthetic procedure, producing crude product and complex mixtures of highly related impurities with minimal side-chain structure variability. It is therefore crucial for the overall success of industrial/pharmaceutical application to develop an effective purification process. In this communication, we present the newly developed water-in-oil reversed micelles and microemulsion system-based crystallization process. Physiochemical properties of the presented crystallization media were varied through surfactants and water composition, and the impact on efficiency was measured through final variation of these two parameters. Using precisely defined properties of the dispersed water phase in crystallization media, a highly efficient separation process in terms of selectivity and yield was developed. Small-angle X-ray scattering, high-performance liquid chromatography, mass spectrometry, and scanning electron microscopy were used to monitor and analyze the separation processes and orlistat products obtained. Typical process characteristics, especially selectivity and yield in regard to reference examples, were compared and discussed. Copyright © 2012 Wiley Periodicals, Inc.

  13. Escherichia coli-based expression system for the heterologous expression and purification of the elicitin β-cinnamomin from Phytophthora cinnamomi.

    Science.gov (United States)

    Hofzumahaus, Sebastian; Schallmey, Anett

    2013-08-01

    Elicitins are sterol carrier proteins from the Oomycete genera Phytophthora and Phytium and elicit a hypersensitive response in many economically important plants, in some cases causing a systemic acquired resistance. Their recombinant expression in bacteria is complicated by the presence of three disulfide bonds in the elicitin structure. In consequence, elicitins have so far only been produced in soluble form by isolation from native Phytophthora or Phytium strains or by recombinant expression in the yeast Pichia pastoris. Here, for the first time, we report the soluble expression of the elicitin β-cinnamomin from Phytophthora cinnamomi in Escherichia coli by secretion of the protein into the periplasm. β-Cinnamomin yields have been significantly improved after careful selection of the optimum secretion signal sequence. In total, 17.6 mg β-cinnamomin per liter cell culture have been obtained in shake flasks with the secretion signal sequence of the maltose-binding protein MalE from E. coli. Furthermore, by making use of a C-terminal His-tag, β-cinnamomin purification has been significantly simplified with only one step of immobilized metal ion affinity chromatography yielding protein of high purity (>90%). The established protocol has further been successfully applied to the soluble expression of another elicitin.

  14. 用FPLC系统提纯可溶型Ⅱ型胶原蛋白%Isolation and purification of soluble typeⅡcollagen with FPLC system

    Institute of Scientific and Technical Information of China (English)

    钱娴娟; 胡永秀; 赵文明

    2001-01-01

    Objective To isolate and purify soluble type Ⅱcollagen by FPLCsystem. Methods Xiphoid cartilage of chickens was digested with pepsin and was centrifuged to obtain the rough protein solution. Then, FPLC system was adopted to carry on DEAE ion exchange chromatography and desalting chromatography in order to purify the protein. Results It was found that the bands of CⅡsamples and standard CⅡwere at the same location by SDS-PAGE. Conclusions The improved method we adopted had significant advantages such as the high purification efficiency,excellent stability and the fast and simple working process compared with traditional method.%目的 采用FPLC系统提取纯化可溶型Ⅱ型胶原蛋白(CⅡ)。方法 采用鸡剑突软骨,经胃蛋白酶消化离心,再使用FPLC系统进行DEAESepharoseFF高效液相离子交换层析和脱盐柱脱盐,得到纯化的CⅡ。结果 自提的CⅡ纯品与CⅡ标准品电泳结果带型一致。结论 本法提纯蛋白效率高,重复性好,快速简便。

  15. ERP II: a conceptual framework for next-generation enterprise systems?

    DEFF Research Database (Denmark)

    Møller, Charles

    2005-01-01

    proposes a conceptual framework for extended enterprise resource planning (ERP II). The aim of this model is to compile present ES concepts into a comprehensive outline of ERP II, thus composing a generic map and taxonomy for corporate-wide enterprise systems. Research limitations/implications - The paper...

  16. A steerable/distance enhanced penetrometer delivery system: Phase II. Topical report

    Energy Technology Data Exchange (ETDEWEB)

    Amini, A.; Shenhar, J.; Lum, K.D.

    1996-05-01

    This report summarizes the phase II work on the Position Location Device (POLO) for penetrometers. Phase II was carried out to generate an integrated design of a full-scale steerable/distance enhanced penetrometer delivery system. Steering provides for the controlled and directional use of the penetrometer, while vibratory thrusting can provide greater penetration ability.

  17. H-I 21 cm absorption in Mg (II)-selected systems at moderate redshifts

    NARCIS (Netherlands)

    Lane, WM; Briggs, FH; Hibbard, JE; Rupen, MP; VanGorkom, JH

    2001-01-01

    We present results from a Westerbork Synthesis Radio Telescope (WSRT) survey for HI 21 cm absorption in 62 MgII-selected absorption systems, among which we identify three now HI 21cm absorbers. We use the redshift evolution of the number density of MgII-absorbers and the Hi detection rate to estimat

  18. Purification of contaminated groundwater by membrane technology

    Energy Technology Data Exchange (ETDEWEB)

    Youn, In Soo; Chung, Chin Ki; Kim, Byoung Gon [Korea Institute of Geology Mining and Materials, Taejon (Korea, Republic of)

    1996-12-01

    The objective of this study is to apply the membrane separation technology to the purification of contaminated ground water in Korea. Under this scope, the purification was aimed to the drinking water level. The scale of the membrane system was chosen to a small filtration plant for local clean water supplies and/or heavy purifiers for buildings and public uses. The actual conditions of ground water contamination in Korea was surveyed to determine the major components to remove under the drinking water requirements. To set up a hybrid process with membrane methods, conventional purification methods were also investigated for the comparison purpose. The research results are summarized as follows : 1) Contamination of the groundwater in Korea has been found to be widespread across the country. The major contaminant were nitrate, bacteria, and organic chlorides. Some solvents and heavy metals are also supposed to exist in the ground water of industrial complexes, cities, and abandoned mines. 2) The purification methods currently used in public filtration plants appear not to be enough for new contaminants from recent industrial expanding. The advanced purification technologies generally adopted for this problem have been found to be unsuitable due to their very complicated design and operation, and lack of confidence in the purification performance. 3) The reverse osmosis tested with FilmTec FT30 membrane was found to remove nitrate ions in water with over 90 % efficiency. 4) The suitable membrane process for the contaminated groundwater in Korea has been found to be the treatments composed of activated carbon, microfiltration, reverse osmosis or ultrafiltration, and disinfection. The activated carbon treatment could be omitted for the water of low organic contaminants. The microfiltration and the reverse osmosis treatments stand for the conventional methods of filtration plants and the advanced methods for hardly removable components, respectively. It is recommended

  19. Comparison Between the Sonic-Sys System and Inlay in II Class

    OpenAIRE

    Feder, T.; Mierzwinska-Nastalska, E; Adamczyk-Sosinska, E.; Gladkowski, J.; Siedlecki, M.

    2002-01-01

    In everyday dental practice we often face the problem of class II restorations. Large caries create serious problems with regard to obtaining correct contact points, marginal fit (expecially in the gingival area) and good resin polymerisation. In such cases usage of the Sonic-Sys system and inlays seems to be the best solution. The aim of this poster is to present a manual of the Sonic-Sys system, as well as comparison of the Empress II and Sonic-Sys system inlays in class II restorations....

  20. Separation of xylose and glucose using an integrated membrane system for enzymatic cofactor regeneration and downstream purification

    DEFF Research Database (Denmark)

    Morthensen, Sofie Thage; Sigurdardóttir, Sigyn Björk; Meyer, Anne S.

    2017-01-01

    Mixtures of xylose, glucose and pyruvate were fed to a membrane bioreactor equipped with a charged NF membrane (NTR 7450). Value-added products were obtained in the reactor via enzymatic cofactor-dependent catalysis of glucose to gluconic acid and pyruvate to lactic acid, respectively. The initial...... cofactor (NADH) concentration could be decreased to 10% of the stoichiometric value (relative to glucose) without compromising process time and substrate conversion via i) efficient cofactor regeneration and ii) high retention of cofactor (R=0.98) in the membrane bioreactor. Furthermore, accumulation...

  1. A Novel Aqueous Two Phase System Composed of a Thermo-Separating Polymer and an Organic Solvent for Purification of Thermo-Acidic Amylase Enzyme from Red Pitaya (Hylocereus polyrhizus Peel

    Directory of Open Access Journals (Sweden)

    Mehrnoush Amid

    2014-05-01

    Full Text Available The purification of thermo-acidic amylase enzyme from red pitaya (Hylocereus polyrhizus peel for the first time was investigated using a novel aqueous two-phase system (ATPS consisting of a thermo-separating copolymer and an organic solvent. The effectiveness of different parameters such as molecular weight of the thermo-separating ethylene oxide-propylene oxide (EOPO copolymer and type and concentration of organic solvent on the partitioning behavior of amylase was investigated. In addition, the effects of phase components, volume ratio (VR, pH and crude load of purification factor and yield of amylase were evaluated to achieve the optimum partition conditions of the enzyme. In the novel ATPS method, the enzyme was satisfactorily partitioned into the polymer-rich top phase in the system composed of 30% (w/w EOPO 2500 and 15% (w/w 2-propanol, at a volume ratio of 1.94 and with a crude load scale of 25% (w/w at pH 5.0. Recovery and recycling of components was also measured in each successive step of the ATPS process. The enzyme was successfully recovered by the method with a high purification factor of 14.3 and yield of 96.6% and copolymer was also recovered and recycled at a rate above 97%, making the method was more economical than the traditional ATPS method.

  2. A novel aqueous two phase system composed of a thermo-separating polymer and an organic solvent for purification of thermo-acidic amylase enzyme from red pitaya (Hylocereus polyrhizus) peel.

    Science.gov (United States)

    Amid, Mehrnoush; Manap, Yazid; Zohdi, Nor Khanani

    2014-05-22

    The purification of thermo-acidic amylase enzyme from red pitaya (Hylocereus polyrhizus) peel for the first time was investigated using a novel aqueous two-phase system (ATPS) consisting of a thermo-separating copolymer and an organic solvent. The effectiveness of different parameters such as molecular weight of the thermo-separating ethylene oxide-propylene oxide (EOPO) copolymer and type and concentration of organic solvent on the partitioning behavior of amylase was investigated. In addition, the effects of phase components, volume ratio (VR), pH and crude load of purification factor and yield of amylase were evaluated to achieve the optimum partition conditions of the enzyme. In the novel ATPS method, the enzyme was satisfactorily partitioned into the polymer-rich top phase in the system composed of 30% (w/w) EOPO 2500 and 15% (w/w) 2-propanol, at a volume ratio of 1.94 and with a crude load scale of 25% (w/w) at pH 5.0. Recovery and recycling of components was also measured in each successive step of the ATPS process. The enzyme was successfully recovered by the method with a high purification factor of 14.3 and yield of 96.6% and copolymer was also recovered and recycled at a rate above 97%, making the method was more economical than the traditional ATPS method.

  3. Particle Simulations of DARHT-II Transport System

    Energy Technology Data Exchange (ETDEWEB)

    Poole, B; Chen, Y J

    2001-06-11

    The DARHT-II beam line utilizes a fast stripline kicker to temporally chop a high current electron beam from a single induction LINAC and deliver multiple temporal electron beam pulses to an x-ray converter target. High beam quality needs to be maintained throughout the transport line from the end of the accelerator through the final focus lens to the x-ray converter target to produce a high quality radiographic image. Issues that will affect beam quality such as spot size and emittance at the converter target include dynamic effects associated with the stripline kicker as well as emittance growth due to the nonlinear forces associated with the kicker and various focusing elements in the transport line. In addition, dynamic effects associated with transverse resistive wall instability as well as gas focusing will affect the beam transport. A particle-in-cell code is utilized to evaluate beam transport in the downstream transport line in DARHT-II. External focusing forces are included utilizing either analytic expressions or field maps. Models for wakefields from the beam kicker, transverse resistive wall instability, and gas focusing are included in the simulation to provide a more complete picture of beam transport in DARHT-II. From these simulations, for various initial beam loads based on expected accelerator performance the temporally integrated target spot size and emittance can be estimated.

  4. Particle Simulations of DARHT-II Transport System

    Energy Technology Data Exchange (ETDEWEB)

    Poole, B; Chen, Y J

    2001-06-11

    The DARHT-II beam line utilizes a fast stripline kicker to temporally chop a high current electron beam from a single induction LINAC and deliver multiple temporal electron beam pulses to an x-ray converter target. High beam quality needs to be maintained throughout the transport line from the end of the accelerator through the final focus lens to the x-ray converter target to produce a high quality radiographic image. Issues that will affect beam quality such as spot size and emittance at the converter target include dynamic effects associated with the stripline kicker as well as emittance growth due to the nonlinear forces associated with the kicker and various focusing elements in the transport line. In addition, dynamic effects associated with transverse resistive wall instability as well as gas focusing will affect the beam transport. A particle-in-cell code is utilized to evaluate beam transport in the downstream transport line in DARHT-II. External focusing forces are included utilizing either analytic expressions or field maps. Models for wakefields from the beam kicker, transverse resistive wall instability, and gas focusing are included in the simulation to provide a more complete picture of beam transport in DARHT-II. From these simulations, for various initial beam loads based on expected accelerator performance the temporally integrated target spot size and emittance can be estimated.

  5. Precious Metals in SDSS Quasar Spectra II: Tracking the Evolution of Strong, 0.4 < z < 2.3 MgII Absorbers with Thousands of Systems

    CERN Document Server

    Seyffert, Eduardo N; Simcoe, Robert A; O'Meara, John M; Kao, Melodie M; Prochaska, J Xavier

    2013-01-01

    We have performed an analysis of over 34,000 MgII doublets at 0.36 2.3, while the W_r >= 1 \\AA absorber line density grew, dN_MgII/dX, by roughly 45%. Finally, we explore the different evolution of various absorber populations---damped Lyman-alpha absorbers, Lyman-limit systems, strong CIV absorbers, and strong and weaker MgII systems---across cosmic time (0 < z < 6).

  6. Update of the trigger system of the PandaX-II experiment

    Science.gov (United States)

    Wu, Q.; Ren, X.; Chen, X.; Ji, X.; Liu, J.; Lei, S.; Wang, M.; Xiao, M.; Xie, P.; Yan, B.; Yang, Y.

    2017-08-01

    PandaX-II experiment is a dark matter direct detection experiment using about half-ton of liquid xenon as the sensitive target. The electrical pulses detected by photomultiplier tubes from scintillation photons of xenon are recorded by waveform digitizers. The data acquisition of PandaX-II relies on a trigger system that generates common trigger signals for all waveform digitizers. Previously an analog device-based trigger system was used for the data acquisition system. In this paper we present a new FPGA-based trigger system. The design of this system and trigger algorithms are described. The performance of this system on real data is presented.

  7. Using aerated gravel-packed contact bed and constructed wetland system for polluted river water purification: A case study in Taiwan

    Science.gov (United States)

    Lin, J. L.; Tu, Y. T.; Chiang, P. C.; Chen, S. H.; Kao, C. M.

    2015-06-01

    The Ju-Liao Stream is one of the most contaminated streams in Kaohsiung City, Taiwan. A constructed wetland (CW) system was built in 2010 for polluted stream water purification and ecosystem improvement. An aerated gravel-packed contact bed (CB) system was built in 2011 and part of the stream water was treated by the CB before discharging to the CW. The influent rates of the CW and CB were approximately 5570 and 900 m3/d, respectively. The CW contained one free-water surface basin planted with emergent wetland plants, followed by the plug-flow channel-shaped free-water surface basin planted with emergent and floating wetland plants. The mean measured hydraulic loading rate (HLR), hydraulic retention time (HRT), water depth, and total volume of wetland system were 1.7 m/d, 0.68 d, 0.7 m, and 4400 m3, respectively. The aeration zone of the CB system had a dimension of 24 m (L) × 8 m (W) × 3 m (H), which was filled with gravels (average diameter = 5 cm) with a porosity of 0.4, and the aeration rate was 7.8 m3/min. Results show that the CB system was able to remove 69% of suspended solid (SS), 86% of biochemical oxygen demand (BOD), and 58% of total nitrogen (TN). Up to 82% of BOD and 27% of TN could be removed in the CW system. Removal efficiency of SS was affected by the growth of chlorophyll a in the CW system due to the growth of algae. The observed first-order decay rates (k) for BOD and TN in CB were 9.3 and 4.2 1/d, and the k values for BOD and TN removal in CW were 2.5 and 0.45 1/d. The high pollutant removal efficiencies in the CB system indicate that the system could enhance the organic and nutrient removal through the biological processes effectively. Sediments contained high total organic matter (1.9-4.5%), sediment total nitrogen (6.4-10.1 g/kg), sediment total phosphorus (0.59-0.94 g/kg), and sediment oxygen demand (0.9-4.1 g O2/m2 d). The organic and nutrient-abundant sediments resulted in reduced conditions (oxidation-reduction potential measurements

  8. Pond systems for the purification and disposal of domestic wastewater from small communities: use, design, operation and maintenance

    CSIR Research Space (South Africa)

    Drews, RJLC

    1983-01-01

    Full Text Available Pond systems are ideally suited for small communities, and for schools, hospitals and other institutions since they are simple and economical to construct, operate and maintain. Numerous pons systems have been designed and commissioned in South...

  9. Purification of monoclonal antibodies, IgG1, from cell culture supernatant by use of metal chelate convective interaction media monolithic columns.

    Science.gov (United States)

    Rajak, Poonam; Vijayalakshmi, M A; Jayaprakash, N S

    2012-12-01

    Monoclonal antibodies (MAbs) have diverse applications in diagnostics and therapeutics. The recent advancement in hybridoma technology for large-scale production of MAbs in bioreactors demands rapid and efficient purification methods. Conventional affinity purification systems have drawbacks of low flow rates and denaturation of antibodies owing to harsh elution conditions. Here, we attempted purification of MAbs by use of a high-throughput metal-chelate methacrylate monolithic system. Monolithic macroporous convective interaction media-iminodiacetate (CIM-IDA) disks immobilized with four different metal ions (Cu²⁺, Ni²⁺, Zn²⁺ and Co²⁺) were used and evaluated for purification of anti-human serum albumin IgG1 mouse MAbs from cell culture supernatant after precipitation with 50% ammonium sulfate. Elution with 10 mM imidazole in the equilibration buffer (25 mM MMA = MOPS (Morpholino propane sulfonic acid) + MES (Morpholino ethane sulfonic acid) + Acetate + 0.5 M NaCl, pH 7.4) resulted in a purification of 25.7 ± 2.9-fold and 32.5 ± 2.6-fold in experiments done using Zn²⁺ and Co²⁺ metal ions, respectively. The highest recovery of 85.4 ± 1.0% was obtained with a CIM-IDA-Zn(II) column. SDS-PAGE, ELISA and immuno-blot showed that the antibodies recovered were pure, with high antigen-binding efficiency. Thus, metal chelate CIM monoliths could be a potential alternative to conventional systems for fast and efficient purification of MAbs from the complex cell culture supernatant.

  10. Zn(II, Mn(II and Sr(II Behavior in a Natural Carbonate Reservoir System. Part II: Impact of Geological CO2 Storage Conditions

    Directory of Open Access Journals (Sweden)

    Auffray B.

    2016-07-01

    Full Text Available Some key points still prevent the full development of geological carbon sequestration in underground formations, especially concerning the assessment of the integrity of such storage. Indeed, the consequences of gas injection on chemistry and petrophysical properties are still much discussed in the scientific community, and are still not well known at either laboratory or field scale. In this article, the results of an experimental study about the mobilization of Trace Elements (TE during CO2 injection in a reservoir are presented. The experimental conditions range from typical storage formation conditions (90 bar, supercritical CO2 to shallower conditions (60 and 30 bar, CO2 as gas phase, and consider the dissolution of the two carbonates, coupled with the sorption of an initial concentration of 10−5 M of Zn(II, and the consequent release in solution of Mn(II and Sr(II. The investigation goes beyond the sole behavior of TE in the storage conditions: it presents the specific behavior of each element with respect to the pressure and the natural carbonate considered, showing that different equilibrium concentrations are to be expected if a fluid with a given concentration of TE leaks to an upper formation. Even though sorption is evidenced, it does not balance the amount of TE released by the dissolution process. The increase in porosity is clearly evidenced as a linear function of the CO2 pressure imposed for the St-Emilion carbonate. For the Lavoux carbonate, this trend is not confirmed by the 90 bar experiment. A preferential dissolution of the bigger family of pores from the preexisting porosity is observed in one of the samples (Lavoux carbonate while the second one (St-Emilion carbonate presents a newly-formed family of pores. Both reacted samples evidence that the pore network evolves toward a tubular network type.

  11. PMT response drift of ATLAS Tile Laser II calibration system: an introduction of a new method

    CERN Document Server

    Di Gregorio, Giulia

    2016-01-01

    In this article I describe the performance of the monitoring diodes of the Laser II system, a new system for run II used to calibrate the gain variation of PMTs in between two cesium scan. I also show a new method to measure the PMT drift response that it is compared to the method used up to now (Clermont-Ferrant) corrected with the Pisa method. The agreement between the two method is within 0.2%.

  12. PERCEPT-II: smartphone based indoor navigation system for the blind.

    Science.gov (United States)

    Ganz, Aura; Schafer, James M; Tao, Yang; Wilson, Carole; Robertson, Meg

    2014-01-01

    In this paper we introduce PERCEPT-II, a low cost and user friendly indoor navigation system for blind and visually impaired users. Using an Android Smartphone that runs PERCEPT-II application with accessibility features, the blind user obtains navigation instructions to the chosen destination when touching specific landmarks tagged with Near Field Communication tags. The system was deployed and tested in a large building at the University of Massachusetts at Amherst.

  13. An Optical Reflector System for the CANGAROO-II Telescope

    CERN Document Server

    Kawachi, A

    1999-01-01

    We have developed light and durable mirrors made of CFRP (Carbon Fiber Reinforced Plastics) laminates for the reflector of the new CANGAROO-II 7 m telescope. The reflector has a parabolic shape (F/1.1) with a 30 m^2 effective area which consists of 60 small spherical mirrors. The attitude of each mirror can be remotely adjusted by stepping motors. After the first adjustment work, the re ector offers a point image of about 0.14 degree (FWHM) on the optic axis. The telescope has been in operation since May 1999 with an energy threshold of ~ 300 GeV.

  14. Evaluation of Laser Stabilization and Imaging Systems for LCLS-II - Final Paper

    Energy Technology Data Exchange (ETDEWEB)

    Barry, Matthew [Auburn Univ., AL (United States)

    2015-08-20

    By combining the top performing commercial laser beam stabilization system with the most ideal optical imaging configuration, the beamline for the Linear Accelerator Coherent Light Source II (LCLS-II) will deliver the highest quality and most stable beam to the cathode. To determine the optimal combination, LCLS-II beamline conditions were replicated and the systems tested with a He-Ne laser. The Guidestar-II and MRC active laser beam stabilization systems were evaluated for their ideal positioning and stability. Both a two and four lens optical imaging configuration was then evaluated for beam imaging quality, magnification properties, and natural stability. In their best performances when tested over fifteen hours, Guidestar-II kept the beam stable over approximately 70-110um while the MRC system kept it stable over approximately 90-100um. During short periods of time, Guidestar-II kept the beam stable between 10-20um, but was more susceptible to drift over time, while the MRC system maintained the beam between 30-50um with less overall drift. The best optical imaging configuration proved to be a four lens system that images to the iris located in the cathode room and from there, imaged to the cathode. The magnification from the iris to the cathode was 2:1, within an acceptable tolerance to the expected 2.1:1 magnification. The two lens configuration was slightly more stable in small periods of time (less than 10 minutes) without the assistance of a stability system, approximately 55um compared to approximately 70um, but the four lens configurations beam image had a significantly flatter intensity distribution compared to the two lens configuration which had a Gaussian distribution. A final test still needs to be run with both stability systems running at the same time through the four lens system. With this data, the optimal laser beam stabilization system can be determined for the beamline of LCLS-II.

  15. Ocean Thermal Energy Conservation (OTEC) power system development (PDS) II. Preliminary design report

    Energy Technology Data Exchange (ETDEWEB)

    1979-08-10

    This report documents the results and conclusions of the PDS II, Phase I, preliminary design of a 10 MWe OTEC power system, using enhanced plate type heat exchangers, and of representative 0.2 MWe test articles. It further provides the documentation (specifications, drawings, trade studies, etc.) resulting from the design activities. The data and discussions of the technical concepts are organized to respond to the PDS II, Phase II proposal evaluation criteria. This volume, which specifically addresses the three evaluation categories (heat exchangers, rotating machinery, and power system configuration and performance) is an integral part of the Phase II plans (proposal) which describe the technical approach to delivering test articles to OTEC-1. In addition, there is a section which addresses power system cost and net energy analysis and another which discusses the results of stainless steel feasibility studies. Supporting documentation is contained in two appendix volumes.

  16. Size-exclusion chromatography as a linear transfer system: purification of human influenza virus as an example.

    Science.gov (United States)

    Kalbfuss, Bernd; Flockerzi, Dietrich; Seidel-Morgenstern, Andreas; Reichl, Udo

    2008-09-15

    Preparative size-exclusion chromatography suffers from low selectivity and productivity. Empirical optimization of operating conditions constitutes a laborious task due to many parameters. Here, a modeling framework based on linear systems theory is presented for predicting the influence of volume overloading. Impulse-responses characterizing system behavior are derived from experimental data by maximum entropy deconvolution. Theoretical derivations are validated experimentally by study of a model system and chromatography of human influenza virus. By application of the theory it is demonstrated how group separation operations can be optimized with respect to yield, purity, productivity and dilution of the product.

  17. Performance of the ATLAS Tile LaserII Calibration System

    CERN Document Server

    AUTHOR|(INSPIRE)INSPIRE-00124895; The ATLAS collaboration

    2015-01-01

    The new laser calibration system of the ATLAS Tile hadron calorimeter is presented. The perfomances of the calibration and monitor tools internal to the laser system are given in terms of operation time stability. The use of the laser system in the normal Tile calibration procedures is also described.

  18. A Wind Farm Electrical Systems Evaluation with EeFarm-II

    NARCIS (Netherlands)

    Pierik, J.; Axelsson, U.; Eriksson, E.; Salomonsson, D.; Bauer, P.; Czech, B.

    2010-01-01

    EeFarm-II is used to evaluate 13 different electrical systems for a 200 MW wind farm with a 100 km connection to shore. The evaluation is based on component manufacturer data of 2009. AC systems are compared to systems with DC connections inside the wind farm and DC connection to shore. Two options

  19. Purification of functionalized DNA origami nanostructures.

    Science.gov (United States)

    Shaw, Alan; Benson, Erik; Högberg, Björn

    2015-05-26

    The high programmability of DNA origami has provided tools for precise manipulation of matter at the nanoscale. This manipulation of matter opens up the possibility to arrange functional elements for a diverse range of applications that utilize the nanometer precision provided by these structures. However, the realization of functionalized DNA origami still suffers from imperfect production methods, in particular in the purification step, where excess material is separated from the desired functionalized DNA origami. In this article we demonstrate and optimize two purification methods that have not previously been applied to DNA origami. In addition, we provide a systematic study comparing the purification efficacy of these and five other commonly used purification methods. Three types of functionalized DNA origami were used as model systems in this study. DNA origami was patterned with either small molecules, antibodies, or larger proteins. With the results of our work we aim to provide a guideline in quality fabrication of various types of functionalized DNA origami and to provide a route for scalable production of these promising tools.

  20. Electron beam silicon purification

    Energy Technology Data Exchange (ETDEWEB)

    Kravtsov, Anatoly [SIA ' ' KEPP EU' ' , Riga (Latvia); Kravtsov, Alexey [' ' KEPP-service' ' Ltd., Moscow (Russian Federation)

    2014-11-15

    Purification of heavily doped electronic grade silicon by evaporation of N-type impurities with electron beam heating was investigated in process with a batch weight up to 50 kilos. Effective temperature of the melt, an indicative parameter suitable for purification process characterization was calculated and appeared to be stable for different load weight processes. Purified material was successfully approbated in standard CZ processes of three different companies. Each company used its standard process and obtained CZ monocrystals applicable for photovoltaic application. These facts enable process to be successfully scaled up to commercial volumes (150-300 kg) and yield solar grade silicon. (copyright 2014 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim) (orig.)

  1. Five-Year Safety and Performance Results from the Argus II Retinal Prosthesis System Clinical Trial.

    Science.gov (United States)

    da Cruz, Lyndon; Dorn, Jessy D; Humayun, Mark S; Dagnelie, Gislin; Handa, James; Barale, Pierre-Olivier; Sahel, José-Alain; Stanga, Paulo E; Hafezi, Farhad; Safran, Avinoam B; Salzmann, Joel; Santos, Arturo; Birch, David; Spencer, Rand; Cideciyan, Artur V; de Juan, Eugene; Duncan, Jacque L; Eliott, Dean; Fawzi, Amani; Olmos de Koo, Lisa C; Ho, Allen C; Brown, Gary; Haller, Julia; Regillo, Carl; Del Priore, Lucian V; Arditi, Aries; Greenberg, Robert J

    2016-10-01

    The Argus II Retinal Prosthesis System (Second Sight Medical Products, Inc, Sylmar, CA) was developed to restore some vision to patients blind as a result of retinitis pigmentosa (RP) or outer retinal degeneration. A clinical trial was initiated in 2006 to study the long-term safety and efficacy of the Argus II System in patients with bare or no light perception resulting from end-stage RP. Prospective, multicenter, single-arm clinical trial. Within-patient controls included the nonimplanted fellow eye and patients' native residual vision compared with their vision with the Argus II. Thirty participants in 10 centers in the United States and Europe. The worse-seeing eye of blind patients was implanted with the Argus II. Patients wore glasses mounted with a small camera and a video processor that converted images into stimulation patterns sent to the electrode array on the retina. The primary outcome measures were safety (the number, seriousness, and relatedness of adverse events) and visual function, as measured by 3 computer-based, objective tests. Secondary measures included functional vision performance on objectively scored real-world tasks. Twenty-four of 30 patients remained implanted with functioning Argus II Systems at 5 years after implantation. Only 1 additional serious adverse event was experienced after the 3-year time point. Patients performed significantly better with the Argus II on than off on all visual function tests and functional vision tasks. The 5-year results of the Argus II trial support the long-term safety profile and benefit of the Argus II System for patients blind as a result of RP. The Argus II is the first and only retinal implant to have market approval in the European Economic Area, the United States, and Canada. Copyright © 2016 American Academy of Ophthalmology. Published by Elsevier Inc. All rights reserved.

  2. Experience with infrasonic purification systems for DENOX reactor purification at Tiefstack heating power station; Erfahrungen zum Einsatz von Infraschall-Reinigungsanlagen fuer die Abreinigung von DENOX-Reaktoren im HKW Tiefstack

    Energy Technology Data Exchange (ETDEWEB)

    Valckenaere, J.; Basener, H.

    1999-07-01

    Solid fuels, whether brown coal, coal, or refuse-derived fuels, cause ash deposition on the boiler heating surfaces. Common purification methos are soot blowoff, pebble cleaning, chemical or mechanical cleaning and, if nothing succeeds, shutoff and manual cleaning. All these techniques are uneconomical as they result in reduced boiler capacities between blowoff and purification cycles. They also cause wear of the boiler which necessitates costly maintenance and repair. The authors propose a new technology in which the dust or soot particles are kept in motion in order to prevent deposition. Infrasonic techniques generate high-energy waves with higher amplitudes which are necessary for inducing ash particle movement. The sound propagates evenly in all directions and has excellent reflection characteristics. Even otherwise inaccessible parts of the boiler, heat exchanger or catalytic converter are reached, ash deposition is prevented, and the boiler is kept clean. [German] Bei der Energieerzeugung verbrennen feststoffgefeuerte Kraftwerke und Muellverbrennungsanlagen Brennstoffe (Braunkohle, Steinkohle, Muell,...), die Reinigungsprobleme durch Ascheablagerungen auf den Kesselheizflaechen verursachen. Die derzeitige Reinigung im Betrieb erfolgt durch Russblasen, Kugelreinigung, chemische und mechanische Reinigung und wenn das nicht hilft, bleibt meistens nur Abstellen und Handreinigung. Konventionelle Reinigungsmethoden sind unwirtschaftlich, weil der Kesselwirkungsgrad zwischen jedem Blas- oder Reinigungszyklus absinkt. Diese Reinigungsarten verursachen einen Verschleiss im Kessel, so dass hohe Wartungs- und Reparaturkosten anfallen. Insgesamt liegt der Energieverbrauch z.B. beim Russblasen zwischen 1 bis 8% des erzeugten Dampfes je nach Brennstoff und Kessel. Die Kesselbetreiber wuenschen aber durchgaengig saubere Heizflaechen, moeglichst wenig Russblasen, keinen Verschleiss im Kessel, einen hohen Wirkungsgrad und vor allem keine Stillstaende. Das Ziel ist die

  3. NDCX-II PULSED POWER SYSTEM AND INDUCTION CELLS

    Energy Technology Data Exchange (ETDEWEB)

    Waldron, W.L.; Reginato, L.L.; Leitner, M.

    2009-06-01

    The Heavy Ion Fusion Science Virtual National Laboratory (HIFS-VNL) is currently finalizing the design of NDCX-II, the second phase of the Neutralized Drift Compression Experiment, which will use an ion beam to explore Warm Dense Matter (WDM) and Inertial Fusion Energy (IFE) target hydrodynamics. The ion induction accelerator will include induction cells and Blumleins from the decommissioned Advanced Test Accelerator (ATA) at Lawrence Livermore National Laboratory (LLNL). A test stand has been built at Lawrence Berkeley National Laboratory (LBNL) to test refurbished ATA induction cells and pulsed power hardware for voltage holding and ability to produce various compression and acceleration waveforms. The performance requirements, design modifications, and test results will be presented.

  4. Production and purification of staphylococcal nuclease in Lactococcus lactis using a new expression-secretion system and a pH-regulated mini-reactor

    Directory of Open Access Journals (Sweden)

    Duvignau Thomas

    2010-05-01

    Full Text Available Abstract Background Staphylococcal (or micrococcal nuclease or thermonuclease (SNase or Nuc is a naturally-secreted nucleic acid degrading enzyme that participates in Staphylococcus aureus spread in the infected host. Purified Nuc protein can be used as an exogenous reagent to clear cellular extracts and improve protein purification. Here, a recombinant form of Nuc was produced and secreted in a Gram-positive host, Lactococcus lactis, and purified from the culture medium. Results The gene segment corresponding to the S. aureus nuclease without its signal peptide was cloned in an expression-secretion vector. It was then fused to a lactococcal sequence encoding a signal peptide, and expressed under the control of a lactococcal promoter that is inducible by zinc starvation. An L. lactis subsp cremoris model strain (MG1363 transformed with the resulting plasmid was grown in either of two media (GM17v and CDM that are free of animal compounds, allowing GMP (Good Manufacturing Practice production. Induction conditions (concentration of the metal chelator EDTA and timing of addition in small-scale pH-regulated fermentors were optimized using LacMF (Lactis Multi-Fermentor, a home-made parallel fermentation control system able to monitor 12 reactors simultaneously. Large amounts of recombinant Nuc (rNuc were produced and secreted in both media, and rNuc was purified from GM17v medium in a single-step procedure. Conclusions In L. lactis, rNuc production and secretion were optimal after induction by 0.5 mM EDTA in small scale (200 mL GM17v exponential phase cultures (at an OD600 of 2, leading to a maximal protein yield of 210 mg per L of culture medium. Purified rNuc was highly active, displaying a specific activity of 2000 U/mg.

  5. Conceptual design and systems analysis of photovoltaic power systems. Volume II. Systems. Revised final report

    Energy Technology Data Exchange (ETDEWEB)

    Pittman, P.F.

    1977-03-01

    Conceptual designs were made and analyses were performed on three types of solar photovoltaic power systems. Included were Residential (1--10 kW), Intermediate (0.1--10 MW), and Central (50--1000 MW) Power Systems to be installed in the 1985 to 2000 time period. Detailed descriptions of each of the three systems studied, descriptions of the necessary subsystems, and discussions of the interfaces between them are presented. Included also are descriptions of system performance and system cost used to perform an economic analysis which assesses the value of each system.

  6. A Wind Farm Electrical Systems Evaluation with EeFarm-II

    Directory of Open Access Journals (Sweden)

    Jan Pierik

    2010-03-01

    Full Text Available EeFarm-II is used to evaluate 13 different electrical systems for a 200 MW wind farm with a 100 km connection to shore. The evaluation is based on component manufacturer data of 2009. AC systems are compared to systems with DC connections inside the wind farm and DC connection to shore. Two options have the best performance for this wind farm size and distance: the AC system and the system with a DC connection to shore. EeFarm-II is a user friendly computer program for wind farm electrical and economic evaluation. It has been built as a Simulink Library in the graphical interface of Matlab-Simulink. EeFarm-II contains models of wind turbines, generators, transformers, AC cables, inductors, nodes, splitters, PWM converters, thyristor converters, DC cables, choppers and statcoms.

  7. Diverse manganese(II)-oxidizing bacteria are prevalent in drinking water systems.

    Science.gov (United States)

    Marcus, Daniel N; Pinto, Ameet; Anantharaman, Karthik; Ruberg, Steven A; Kramer, Eva L; Raskin, Lutgarde; Dick, Gregory J

    2016-12-09

    Manganese (Mn) oxides are highly reactive minerals that influence the speciation, mobility, bioavailability and toxicity of a wide variety of organic and inorganic compounds. Although Mn(II)-oxidizing bacteria are known to catalyze the formation of Mn oxides, little is known about the organisms responsible for Mn oxidation in situ, especially in engineered environments. Mn(II)-oxidizing bacteria are important in drinking water systems, including in biofiltration and water distribution systems. Here, we used cultivation dependent and independent approaches to investigate Mn(II)-oxidizing bacteria in drinking water sources, a treatment plant and associated distribution system. We isolated 29 strains of Mn(II)-oxidizing bacteria and found that highly similar 16S rRNA gene sequences were present in all culture-independent datasets and dominant in the studied drinking water treatment plant. These results highlight a potentially important role for Mn(II)-oxidizing bacteria in drinking water systems, where biogenic Mn oxides may affect water quality in terms of aesthetic appearance, speciation of metals and oxidation of organic and inorganic compounds. Deciphering the ecology of these organisms and the factors that regulate their Mn(II)-oxidizing activity could yield important insights into how microbial communities influence the quality of drinking water.

  8. Sodium Purification Device for Production of Tantalum Powder

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    In the process of tantalum powder production it requires pure sodium to reduce potassium fluotantalate, thus the design of a sodium purification device is improved, later it is built and commissioned. The device includes sodium transportation tank, storage tank, filter, cold trap, final storage tank, metering tank, regulating valve, argon purification system, electric control panel and instrument. Industrial purity sodium is purified, the impurities in the sodium were reduced to very

  9. HOUSEHOLD PURIFICATION OF FLUORIDE CONTAMINATED MAGADI (TRONA)

    DEFF Research Database (Denmark)

    1997-01-01

    Purification of fluoride contaminated magadi is studied using bone char sorption and calcium precipitation. The bone char treatment is found to be workable both in columns and in batches where the magadi is dissolved in water prior to treatment. The concentrations in the solutions were 89 g magadi....../L and 95 and 400 mg F/L respectively in natural and synthetic solutions. The fluoride removal capacities observed were 4.6 mg F/g bone char for the column system and 2.7 mg F/g bone char for the batch system in case of synthetic magadi solution. It is however concluded that the batch system is the best...... treatment method. A procedure for purification of fluoride contaminated magadi at household level is described....

  10. Rhode Island Model Evaluation & Support System: Support Professional. Edition II

    Science.gov (United States)

    Rhode Island Department of Education, 2015

    2015-01-01

    Rhode Island educators believe that implementing a fair, accurate, and meaningful evaluation and support system for support professionals will help improve student outcomes. The primary purpose of the Rhode Island Model Support Professional Evaluation and Support System (Rhode Island Model) is to help all support professionals do their best work…

  11. Encounters between spherical galaxies - II. Systems with a dark halo

    NARCIS (Netherlands)

    Gonzalez-Garcia, AC; van Albada, TS

    2005-01-01

    We perform N-body simulations of encounters between spherical systems surrounded by a spherical halo. Following a preceding paper with a similar aim, the initial systems include a spherical Jaffe model for the luminous matter and a Hernquist model for the halo. The merger remnants from this sample a

  12. Immobilized palladium(II) ion affinity chromatography for recovery of recombinant proteins with peptide tags containing histidine and cysteine.

    Science.gov (United States)

    Kikot, Pamela; Polat, Aise; Achilli, Estefania; Fernandez Lahore, Marcelo; Grasselli, Mariano

    2014-11-01

    Fusion of peptide-based tags to recombinant proteins is currently one of the most used tools for protein production. Also, immobilized metal ion affinity chromatography (IMAC) has a huge application in protein purification, especially in research labs. The combination of expression systems of recombinant tagged proteins with this robust chromatographic system has become an efficient and rapid tool to produce milligram-range amounts of proteins. IMAC-Ni(II) columns have become the natural partners of 6xHis-tagged proteins. The Ni(II) ion is considered as the best compromise of selectivity and affinity for purification of a recombinant His-tagged protein. The palladium(II) ion is also able to bind to side chains of amino acids and form ternary complexes with iminodiacetic acid and free amino acids and other sulfur-containing molecules. In this work, we evaluated two different cysteine- and histidine-containing six amino acid tags linked to the N-terminal group of green fluorescent protein (GFP) and studied the adsorption and elution conditions using novel eluents. Both cysteine-containing tagged GFPs were able to bind to IMAC-Pd(II) matrices and eluted successfully using a low concentration of thiourea solution. The IMAC-Ni(II) system reaches less than 20% recovery of the cysteine-containing tagged GFP from a crude homogenate of recombinant Escherichia coli, meanwhile the IMAC-Pd(II) yields a recovery of 45% with a purification factor of 13.

  13. Final safety analysis addendum to hazard summary report, experimental breeder reactor No. II (EBR-II): the EBR-II cover-gas cleanup system

    Energy Technology Data Exchange (ETDEWEB)

    Fryer, R M; Monson, L R; Price, C C; Hooker, D W

    1979-04-01

    This report evaluates abnormal and accident conditions postulated for the EBR-II cover-gas cleanup system (CGCS). Major considerations include loss of CGCS function with a high level of cover-gas activity, loss of the liquid-nitrogen coolant required for removing fission products from the cover gas, contamination of the cover gas from sources other than the reactor, and loss of system pressure boundary. Calculated exposures resulting from the maximum hypothetical accident (MHA) are less than 2% of the 25-Rem limit stipulated in U.S. Regulation 10 CFR 100; i.e., a person standing at any point on an exclusion boundary (area radius of 600 m) for 2 h following onset of the postulated release would receive less than 0.45 Rem whole-body dose. The on-site whole-body dose (10 m from the source) would be less than 16 Rem.

  14. Multi-Rate Digital Control Systems with Simulation Applications. Volume II. Computer Algorithms

    Science.gov (United States)

    1980-09-01

    34 ~AFWAL-TR-80-31 01 • • Volume II L IL MULTI-RATE DIGITAL CONTROL SYSTEMS WITH SIMULATiON APPLICATIONS Volume II: Computer Algorithms DENNIS G. J...29 Ma -8 - Volume II. Computer Algorithms ~ / ’+ 44MWLxkQT N Uwe ~~ 4 ~jjskYIF336l5-79-C-369~ 9. PER~rORMING ORGANIZATION NAME AND ADDRESS IPROG AMEL...additional options. The analytical basis for the computer algorithms is discussed in Ref. 12. However, to provide a complete description of the program, some

  15. Nuclear instrumentation system operating experience and nuclear instrument testing in the EBR-II

    Energy Technology Data Exchange (ETDEWEB)

    Yingling, G. E.; Curran, R. N.

    1980-01-01

    In March of 1972 three wide range nuclear channels were purchased from Gulf Atomics Corporation and installed in EBR-II as a test. The three channels were operated as a test until April 1975 when they became a permanent part of the reactor shutdown system. Also described are the activities involved in evaluating and qualifying neutron detectors for LMFBR applications. Included are descriptions of the ANL Components Technology Division Test Program and the EBR-II Nuclear Instrument Test Facilities (NITF) used for the in-reactor testing and a summary of program test results from EBR-II.

  16. Design and Application of Smoke and Dust Purification System for Steel Pipe External Corrosion Host Head%钢管外防腐主机模头烟尘净化系统的设计与应用

    Institute of Scientific and Technical Information of China (English)

    涂君俊

    2014-01-01

    According to environmental pollution problems caused by smoke and dust which appeared in extrusion and winding process of polyethylene/polypropylene and adhesive, a reliable operation smoke and dust purification system was designed. This system consists of plate filter, draught fan, sound proof box, exit silencer, transducer, pressure sensing system, suction hook suite etc. It emphasized the design and function of suction hook and plate filter. The smoke and dust purification system can solve the smoke purification treatment and harmful pollution problem caused from PE/PP heated.%针对钢管在外防腐工艺中聚乙烯(聚丙烯)及胶粘剂挤出过程和缠绕表面过程所产生的烟尘及对车间环境污染的问题,设计了一种工作可靠的烟尘净化系统。烟尘净化系统由板式过滤器、风机及隔音箱、出口消音器、变频器、压力感应系统及吸气罩套件等组成,重点介绍了该系统的主要部件吸气罩和板式过滤器的设计及功能。烟尘净化系统较好地解决了防腐烟尘净化处理及PE/PP受热产生的有害污染问题。

  17. Juvenile idiopathic arthritis (JIA): a screening study to measure class II skeletal pattern, TMJ PDS and use of systemic corticosteroids.

    LENUS (Irish Health Repository)

    Mandall, Nicky A

    2010-03-01

    To screen patients with oligoarticular and polyarticular forms of Juvenile Idiopathic Arthritis (JIA) to determine (i) the severity of their class II skeletal pattern; (ii) temporomandibular joint signs and symptoms and (iii) use of systemic corticosteroids.

  18. [Purification of eutrophic wastewater by Cyperus alternifolius, Coleus blumei and Jasminum sambac planted in a floating phytoremediation system].

    Science.gov (United States)

    Liu, Shizhe; Lin, Dongjiao; Tang, Shujun; Luo, Jian

    2004-07-01

    In a greenhouse study, Cyperus alternifolius, Coleus blumei and Jasminum sambac were cultured in a floating phytoremediation system with plantation cups inserted into a polyfoam plate that floated in the upper part of a tank filled with 100 L domestic wastewater. The contents of chemical oxygen demand (CODCr), total P (T-P), total N (T-N), soluble P(S-P), ammonia-nitrogen (NH4+ -N) and nitrate-nitrogen (NO3- -N) in the domestic wastewater were tested during the growth of these three plants. The results showed that Cyperus alternifolius and Coleus blumei could grow well in the floating phytoremediation system, their dry weight being 285.8% and 371.4% of the initial weight of planting, respectively, but Jasminum sambac could not grow well, being 125.0% of the initial weight of planting. The removal rate of TN by these 3 plants was 68.0%, 62.0% and 45.0%, and that of NO3- -N, CODCr and TP was 98.0%, 80.0% and 92.0%, 78.0%, 66.0% and 55.0%, and 90.6%, 90.5% and 88.0% respectively. Cyperus alternifolius and Coleus blumei had good effects on the removal of pollutants in the floating phytoremediation system.

  19. Extraction and purification of anthraquinones derivatives from Aloe vera L. using alcohol/salt aqueous two-phase system.

    Science.gov (United States)

    Tan, Zhi-jian; Li, Fen-fang; Xu, Xue-lei

    2013-08-01

    An alcohol/salt aqueous two-phase system (ATPS) composed of 1-propanol and (NH4)2SO4 was employed to purify anthraquinones (AQs) extracted from Aloe vera L. The main influencing system parameters such as type of alcohol, type and concentration of salt, temperature and pH were investigated in detail. Under the optimal extraction conditions, AQs can be extracted into alcohol-rich phase with high extraction efficiency, meanwhile majority polysaccharides, proteins, mineral substances and other impurities were extracted into salt-rich phase. Partitioning of AQs is dependent on hydrophobic interaction, hydrogen bond interaction, and salting-out effect in ATPS. Temperature also played a great role in the partitioning. After ATPS extraction, alcohol can be recycled by evaporation; moreover, salt can be recycled by dilution crystallization method. Compared with other liquid-liquid extractions, this alcohol/salt system is much simpler, lower in cost with easier recovery of phase-forming components, which has the potential scale-up in down-processing of active ingredients in plant.

  20. Monte Carlo Simulation for the MAGIC-II System

    CERN Document Server

    Carmona, E; Moralejo, A; Vitale, V; Sobczynska, D; Haffke, M; Bigongiari, C; Otte, N; Cabras, G; De Maria, M; De Sabata, F

    2007-01-01

    Within the year 2007, MAGIC will be upgraded to a two telescope system at La Palma. Its main goal is to improve the sensitivity in the stereoscopic/coincident operational mode. At the same time it will lower the analysis threshold of the currently running single MAGIC telescope. Results from the Monte Carlo simulations of this system will be discussed. A comparison of the two telescope system with the performance of one single telescope will be shown in terms of sensitivity, angular resolution and energy resolution.

  1. The optical reflector system for the CANGAROO-II imaging atmospheric Cherenkov telescope

    CERN Document Server

    Kawachi, A; Jimbo, J; Kamei, S; Kifune, T; Kubo, H; Kushida, J; Le Bohec, S; Miyawaki, K; Mori, M; Nishijima, K; Patterson, J R; Suzuki, R; Tanimori, T; Yanagita, S; Yoshikoshi, T; Yuki, A

    2001-01-01

    A new imaging atmospheric Cherenkov telescope (CANGAROO-II) with a light-weight reflector has been constructed. Light, robust, and durable mirror facets of containing CFRP (Carbon Fiber Reinforced Plastic) laminates were developed for the telescope. The attitude of each facet can be adjusted by stepping motors. In this paper, we describe the design, manufacturing, alignment procedure, and the performance of the CANGAROO-II optical reflector system.

  2. Technical Information on the Carbonation of the EBR-II Reactor, Summary Report Part 2: Application to EBR-II Primary Sodium System and Related Systems

    Energy Technology Data Exchange (ETDEWEB)

    Steven R. Sherman; Collin J. Knight

    2006-03-01

    Residual sodium is defined as sodium metal that remains behind in pipes, vessels, and tanks after the bulk sodium metal has been melted and drained from such components. The residual sodium has the same chemical properties as bulk sodium, and differs from bulk sodium only in the thickness of the sodium deposit. Typically, sodium is considered residual when the thickness of the deposit is less than 5-6 cm. This residual sodium must be removed or deactivated when a pipe, vessel, system, or entire reactor is permanently taken out of service, in order to make the component or system safer and/or to comply with decontamination and decomissioning regulations. As an alternative to the established residual sodium deactivation techniques (steam-and-nitrogen, wet vapor nitrogen, etc.), a technique involving the use of moisture and carbon dioxide has been developed. With this technique, sodium metal is converted into sodium bicarbonate by reacting it with humid carbon dioxide. Hydrogen is emitted as a by-product. This technique was first developed in the laboratory by exposing sodium samples to humidifed carbon dioxide under controlled conditions, and then demonstrated on a larger scale by treating residual sodium within the Experimental Breeder Reactor II (EBR-II) secondary cooling system, followed by the primary cooling system, respectively. The EBR-II facility is located at the Idaho National Laboratory (INL) in southeastern Idaho, USA. This report is Part 2 of a two-part report. This second report provides a supplement to the first report and describes the application of the humdidified carbon dioxide technique ("carbonation") to the EBR-II primary tank, primary cover gas systems, and the intermediate heat exchanger. Future treatment plans are also provided.

  3. DIY soundcard based temperature logging system. Part II: applications

    Science.gov (United States)

    Nunn, John

    2016-11-01

    This paper demonstrates some simple applications of how temperature logging systems may be used to monitor simple heat experiments, and how the data obtained can be analysed to get some additional insight into the physical processes.

  4. Toward autonomous driving: The CMU Navlab. II - Architecture and systems

    Science.gov (United States)

    Thorpe, Charles; Hebert, Martial; Kanade, Takeo; Shafer, Steven

    1991-01-01

    A description is given of EDDIE, the architecture for the Navlab mobile robot which provides a toolkit for building specific systems quickly and easily. Included in the discussion are the annotated maps used by EDDIE and the Navlab's road-following system, called the Autonomous Mail Vehicle, which was built using EDDIE and its annotated maps as a basis. The contributions of the Navlab project and the lessons learned from it are examined.

  5. Identification and estimation algorithm for stochastic neural system. II.

    Science.gov (United States)

    Nakao, M; Hara, K; Kimura, M; Sato, R

    1985-01-01

    The algorithm for identifying the stochastic neural system and estimating the system process which reflects the dynamics of the neural network are presented in this paper. The analogous algorithm has been proposed in our preceding paper (Nakao et al., 1984), which was based on the randomly missed observations of a system process only. Since the previous algorithm mentioned above was subject to an unfavorable effect of consecutively missed observations, to reduce such an effect the algorithm proposed here is designed additionally to observe an intensity process in a neural spike train as the information for the estimation. The algorithm is constructed with the extended Kalman filters because it is naturally expected that a nonlinear and time variant structure is necessary for the filters to realize the observation of an intensity process by means of mapping from a system process to an intensity process. The performance of the algorithm is examined by applying it to some artificial neural systems and also to cat's visual nervous systems. The results in these applications are thought to prove the effectiveness of the algorithm proposed here and its superiority to the algorithm proposed previously.

  6. Zn(II, Mn(II and Sr(II Behavior in a Natural Carbonate Reservoir System. Part I: Impact of Salinity, Initial pH and Initial Zn(II Concentration in Atmospheric Conditions

    Directory of Open Access Journals (Sweden)

    Auffray B.

    2016-07-01

    Full Text Available The sorption of inorganic elements on carbonate minerals is well known in strictly controlled conditions which limit the impact of other phenomena such as dissolution and/or precipitation. In this study, we evidence the behavior of Zn(II (initially in solution and two trace elements, Mn(II and Sr(II (released by carbonate dissolution in the context of a leakage from a CO2 storage site. The initial pH chosen are either equal to the pH of the water-CO2 equilibrium (~ 2.98 or equal to the pH of the water-CO2-calcite system (~ 4.8 in CO2 storage conditions. From this initial influx of liquid, saturated or not with respect to calcite, the batch experiments evolve freely to their equilibrium, as it would occur in a natural context after a perturbation. The batch experiments are carried out on two natural carbonates (from Lavoux and St-Emilion with PCO2 = 10−3.5 bar, with different initial conditions ([Zn(II]i from 10−4 to 10−6 M, either with pure water or 100 g/L NaCl brine. The equilibrium regarding calcite dissolution is confirmed in all experiments, while the zinc sorption evidenced does not always correspond to the two-step mechanism described in the literature. A preferential sorption of about 10% of the concentration is evidenced for Mn(II in aqueous experiments, while Sr(II is more sorbed in saline conditions. This study also shows that this preferential sorption, depending on the salinity, is independent of the natural carbonate considered. Then, the simulations carried out with PHREEQC show that experiments and simulations match well concerning the equilibrium of dissolution and the sole zinc sorption, with log KZn(II ~ 2 in pure water and close to 4 in high salinity conditions. When the simulations were possible, the log K values for Mn(II and Sr(II were much different from those in the literature obtained by sorption in controlled conditions. It is shown that a new conceptual model regarding multiple Trace Elements (TE sorption is

  7. Predictive value of SAPS II and APACHE II scoring systems for patient outcome in a medical intensive care unit

    Directory of Open Access Journals (Sweden)

    Amina Godinjak

    2016-11-01

    Full Text Available Objective. The aim is to determine SAPS II and APACHE II scores in medical intensive care unit (MICU patients, to compare them for prediction of patient outcome, and to compare with actual hospital mortality rates for different subgroups of patients. Methods. One hundred and seventy-four patients were included in this analysis over a oneyear period in the MICU, Clinical Center, University of Sarajevo. The following patient data were obtained: demographics, admission diagnosis, SAPS II, APACHE II scores and final outcome. Results. Out of 174 patients, 70 patients (40.2% died. Mean SAPS II and APACHE II scores in all patients were 48.4±17.0 and 21.6±10.3 respectively, and they were significantly different between survivors and non-survivors. SAPS II >50.5 and APACHE II >27.5 can predict the risk of mortality in these patients. There was no statistically significant difference in the clinical values of SAPS II vs APACHE II (p=0.501. A statistically significant positive correlation was established between the values of SAPS II and APACHE II (r=0.708; p=0.001. Patients with an admission diagnosis of sepsis/septic shock had the highest values of both SAPS II and APACHE II scores, and also the highest hospital mortality rate of 55.1%. Conclusion. Both APACHE II and SAPS II had an excellent ability to discriminate between survivors and non-survivors. There was no significant difference in the clinical values of SAPS II and APACHE II. A positive correlation was established between them. Sepsis/septic shock patients had the highest predicted and observed hospital mortality rate.

  8. Origin and Properties of Strong Mg II Quasar Absorption Line Systems

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    Strong Mg II quasar absorption line systems provide us with a useful tool to understand the gas that plays an important role in galaxy formation. In this paper, placing the theories of galaxy formation in a cosmological context, we present semi-analytic models and Monte-Carlo simulations for strong Mg II absorbers produced in gaseous galactic haloes and/or galaxy discs. We investigate the redshift path density for the MgII absorption lines and the properties of galaxy/absorber pairs, in particular the anti-correlation between the equivalent width of Mg II absorption line and the projected galaxy-to-sightline distance. The simulated result of the mean redshift path density of strong Mg II systems is consistent with the observational result. The fraction of strong Mg II systems arising from galaxy disks is predicted to be ~ 10% of the total. There exists an anti-correlation between the absorption line equivalent and the projected distance of sightline to galaxy center and galaxy luminosity. We determined that the mean absorbing radius Rabs ≈ 29h-1 kpc(LB/LB*)0.35. After taking selection effects into consideration, this becomes Rabs ≈ 38 h-1 kpc(LB/LB*)0.18, which is in good agreement with the observational result. This shows the importance of considering selection effects when comparing models with observations.

  9. High-temperature metal purification using a compact, portable rf heating and levitation system on the wake shield

    Science.gov (United States)

    Hahs, C. A.

    1990-01-01

    The potential use of a compact, battery-operated rf levitator and heating system to purify high-temperature melting materials in space is described. The wake shield now being fabricated for the Space Vacuum Epitaxy Center will provide an Ultra-high vacuum (10(exp -14) Torr hydrogen, 10(exp -14) Torr helium, 10(exp -30) Torr oxygen). The use of the wake shield to purify Nb, Ti, W, Ir, and other metals to a purity level not achievable on earth is described.

  10. Expression, Purification, Crystallization and Molecular Replacement Studies of TorI, an Inhibition Protein of Tor System

    Institute of Scientific and Technical Information of China (English)

    HUANG Wei; YUAN Cai; Ansaldi Mireille; Morelli Xavier; Edward J. Meehan; CHEN Li-Qing; HUANG Ming-Dong

    2007-01-01

    TorI, a Tor system inhibitor acting through protein-protein interaction with the TorR response regulator, is an excisionase that interacts with the integrase and DNA during prophage excision. It has been crystallized by the vapor-diffusion method using polyethylene glycol 3350 as a precipitant at pH 8.5. The X-ray diffraction data sets from the TorI crystal was collected at a resolution of 2.1 (A), using a synchrotron source. The crystal belongs to primitive monoclinic lattice with cell parameters of 46.210(A) × 53.992(A) × 73.561(A)

  11. Commissioning and Early Operation Experience of the NSLS-II Storage Ring RF System

    Energy Technology Data Exchange (ETDEWEB)

    Gao, F.; Rose, J.; Cupolo, J.; Dilgen, T.; Rose, B.; Gash, W.; Ravindranath, V.; Yeddulla, M.; Papu, J.; Davila, P.; Holub, B.; Tagger, J.; Sikora, R.; Ramirez, G.; Kulpin, J.

    2015-05-03

    The National Synchrotron Light Source II (NSLS-II) is a 3 GeV electron X-ray user facility commissioned in 2014. The storage ring RF system, essential for replenishing energy loss per turn of the electrons, consists of digital low level RF controllers, 310 kW CW klystron transmitters, CESR-B type superconducting cavities, as well as a supporting cryogenic system. Here we will report on RF commissioning and early operation experience of the system for beam current up to 200mA.

  12. Computerized Operator Support System – Phase II Development

    Energy Technology Data Exchange (ETDEWEB)

    Ulrich, Thomas A.; Boring, Ronald L.; Lew, Roger T.; Thomas, Kenneth D.

    2015-02-01

    A computerized operator support system (COSS) prototype for nuclear control room process control is proposed and discussed. The COSS aids operators in addressing rapid plant upsets that would otherwise result in the shutdown of the power plant and interrupt electrical power generation, representing significant costs to the owning utility. In its current stage of development the prototype demonstrates four advanced functions operators can use to more efficiently monitor and control the plant. These advanced functions consist of: (1) a synthesized and intuitive high level overview display of system components and interrelations, (2) an enthalpy-based mathematical chemical and volume control system (CVCS) model to detect and diagnose component failures, (3) recommended strategies to mitigate component failure effects and return the plant back to pre-fault status, and (4) computer-based procedures to walk the operator through the recommended mitigation actions. The COSS was demonstrated to a group of operators and their feedback was collected. The operators responded positively to the COSS capabilities and features and indicated the system would be an effective operator aid. The operators also suggested several additional features and capabilities for the next iteration of development. Future versions of the COSS prototype will include additional plant systems, flexible computer-based procedure presentation formats, and support for simultaneous component fault diagnosis and dual fault synergistic mitigation action strategies to more efficiently arrest any plant upsets.

  13. Definition of the Semisubmersible Floating System for Phase II of OC4

    Energy Technology Data Exchange (ETDEWEB)

    Robertson, A.; Jonkman, J.; Masciola, M.; Song, H.; Goupee, A.; Coulling, A.; Luan, C.

    2014-09-01

    Phase II of the Offshore Code Comparison Collaboration Continuation (OC4) project involved modeling of a semisubmersible floating offshore wind system as shown below. This report documents the specifications of the floating system, which were needed by the OC4 participants for building aero-hydro-servo-elastic models.

  14. The ATLAS Data Flow System for LHC Run II

    CERN Document Server

    Kazarov, Andrei; The ATLAS collaboration

    2015-01-01

    After its first shutdown, the LHC will provide pp collisions with increased luminosity and energy. In the ATLAS experiment, the Trigger and Data Acquisition (TDAQ) system has been upgraded to deal with the increased event rates. The Data Flow (DF) element of the TDAQ is a distributed hardware and software system responsible for buffering and transporting event data from the readout system to the High Level Trigger (HLT) and to the event storage. The DF has been reshaped in order to profit from the technological progress and to maximize the flexibility and efficiency of the data selection process. The updated DF is radically different from the previous implementation both in terms of architecture and expected performance. The pre-existing two level software filtering, known as L2 and the Event Filter, and the Event Building are now merged into a single process, performing incremental data collection and analysis. This design has many advantages, among which are: the radical simplification of the architecture, ...

  15. [Purification efficiency of vertical-flow wetland system constructed by cinder and turf substrate on municipal wastewater].

    Science.gov (United States)

    Cui, Lihua; Zhu, Xizhen; Luo, Shiming; Liu, Yihu

    2003-04-01

    Vertical-flow constructed wetlands (VFCWs) system not only has a higher hydraulic loading rate (54-64 cm.d-1), but also has a good removal efficiency for organics, ammonia nitrogen (AN) and total phosphorus (TP). The removal efficiencies of COD, BOD5, AN, and TP for septic tank effluent were 76-87%, 82-92%, 75-85% and 77-91%, respectively, and the average effluent concentrations of COD, BOD5, AN, and TP in the treated effluent were less than 60, 20, 25 and 2.0 mg.L-1, respectively. A comparison of planted and unplanted columns showed that plantation of Cyperus alternifolius could increase the removal rates of AN, TN, and TP by 2-3%, 4-6%, and 10-14%, respectively.

  16. Update: Systemic Diseases and the Cardiovascular System (II). The endocrine system and the heart: a review.

    Science.gov (United States)

    Rhee, Soo S; Pearce, Elizabeth N

    2011-03-01

    Normal endocrine function is essential for cardiovascular health. Disorders of the endocrine system, consisting of hormone hyperfunction and hypofunction, have multiple effects on the cardiovascular system. In this review, we discuss the epidemiology, diagnosis, and management of disorders of the pituitary, thyroid, parathyroid, and adrenal glands, with respect to the impact of endocrine dysfunction on the cardiovascular system. We also review the cardiovascular benefits of restoring normal endocrine function. Copyright © 2010 Sociedad Española de Cardiología. Published by Elsevier Espana. All rights reserved.

  17. Partitioning and purification of extracellular β-1,3-1,4-glucanase in aqueous two-phase systems

    Institute of Scientific and Technical Information of China (English)

    HE Guo-qing; ZHANG Xiu-yan; TANG Xing-jun; CHEN Qi-he; RUAN Hui

    2005-01-01

    The partition behaviors of β-1,3-1,4-glucanase, α-amylase and neutral proteases from clarified and whole fermentation broths of Bacillus subtilis ZJF-1A5 were investigated. An aqueous two-phase system (polyethylene glycol (PEG)/MgSO4)was examined with regard to the effects of PEG molecular weight (MW) and concentration, MgSO4 concentration, pH and NaCl concentration on enzyme partition and extraction. The MW and concentration of PEG were found to have significant effects on enzyme partition and extraction with low MW PEG showing the greatest benefit in the partition and extraction of β-glucanase with the PEG/MgSO4 system. MgSO4 concentration influenced the partition and extraction of β-glucanase significantly. pH had little effect on β-glucanase or proteases partition but affected α-amylase partition when pH was over 7.0. The addition of NaCl had little effect on the partition behavior of β-glucanase but had very significant effects on the partitioning of α-amylase and on the neutral proteases. The partition behaviors of β-glucanase, α-amylase and proteases in whole broth were also investigated and results were similar to those obtained with clarified fermentation broth. A two-step process for purifying β-glucanase was developed, which achieved β-glucanase recovery of 65.3% and specific activity of 14027 U/mg, 6.6 times improvement over the whole broth.

  18. Objective assessment of mammography systems. Part II. Implementation

    Energy Technology Data Exchange (ETDEWEB)

    Hessler, C.; Depeursinge, C.; Grecescu, M.; Pochon, Y.; Raimondi, S.; Valley, J.F.

    1985-07-01

    A quality control program for mammography units was carried out, based on objective tests of image quality and dose. Results are reported for 31 units, including correlation between various parameters. Satisfactory results were obtained in the case of 21 installations; for the other 10, comparison between measured quality parameters and those of the reference system suggested ways in which quality might be improved.

  19. ROMANIAN BANKING SYSTEM EVOLUTION AND BASEL II REQUIREMENTS

    Directory of Open Access Journals (Sweden)

    Mihaela NICOLAU

    2006-01-01

    Full Text Available Before 1989, Romanian Banking System was structured in the specific way of a centralized economy. Restructuring of the banking system took its first step at the end of 1990 when the newly- established bank, Banca Comercială Română, took over retail operations performed previously by the NBR. Simultaneously, some privately-owned banking companies were established and foreign banks’ branches were integrated into the domestic banking activity, the number of banks almost trebling. The unfriendly economic environment, the poor quality of bank managers and shareholders and cumbersome legal procedures led to an increase in tensions, the poor quality of credit portfolio representing the major difficulty of the banking sector. In the past years, NBR tried to control more thebanking activity by implementing international settlements. More over, since Romania is one of the European Union countries, it is absolutely necessary the harmonization of entire economic and financial system to EU regulations. The paper try to present the position of Romanian banking system in the framework of all these transformations.

  20. The ATLAS Trigger System: Ready for Run II

    CERN Document Server

    Czodrowski, Patrick; The ATLAS collaboration

    2015-01-01

    The ATLAS trigger system has been used successfully for data collection in the 2009-2013 Run 1 operation cycle of the CERN Large Hadron Collider (LHC) at center-of-mass energies of up to 8 TeV. With the restart of the LHC for the new Run 2 data-taking period at 13 TeV, the trigger rates are expected to rise by approximately a factor of 5. The trigger system consists of a hardware-based first level (L1) and a software-based high-level trigger (HLT) that reduces the event rate from the design bunch-crossing rate of 40 MHz to an average recording rate of ~ 1kHz. This presentation will give an overview of the upgrades to the ATLAS trigger system that have been implemented during the LHC shutdown period in order to deal with the increased trigger rates while efficiently selecting the physics processes of interest. These upgrades include changes to the L1 calorimeter trigger, the introduction of a new L1 topological trigger module, improvements in the L1 muon system, and the merging of the previously two-level HLT ...

  1. The Thermodynamic Limit of Quantum Coulomb Systems. Part II. Applications

    CERN Document Server

    Hainzl, Christian; Solovej, Jan Philip

    2008-01-01

    In a previous paper, we have developed a general theory of thermodynamic limits. We apply it here to three different Coulomb quantum systems, for which we prove the convergence of the free energy per unit volume. The first system is the crystal for which the nuclei are classical particles arranged periodically in space and only the electrons are quantum particles. We recover and generalize a previous result of Fefferman. In the second example, both the nuclei and the electrons are quantum particles, submitted to a periodic magnetic field. We thereby extend a seminal result of Lieb and Lebowitz. Finally, in our last example we take again classical nuclei but optimize their position. To our knowledge such a system was never treated before. The verification of the assumptions introduced in the previous paper uses several tools which have been introduced before in the study of large quantum systems. In particular, an electrostatic inequality of Graf and Schenker is one main ingredient of our new approach.

  2. Simulation of advanced ultrasound systems using Field II

    DEFF Research Database (Denmark)

    Jensen, Jørgen Arendt

    2004-01-01

    impulse responses is explained. A simulation example for a synthetic aperture spread spectrum flow systems is described. It is shown how the advanced coded excitation can be set up, and how the simulation can be parallelized to reduce the simulation time from 17 months to 391 hours using a 32 CPU Linux...

  3. Ultrasound Vector Flow Imaging: Part II: Parallel Systems

    DEFF Research Database (Denmark)

    Jensen, Jørgen Arendt; Nikolov, Svetoslav Ivanov; Yu, Alfred C. H.;

    2016-01-01

    ultrasound imaging for studying brain function in animals. The paper explains the underlying acquisition and estimation methods for fast 2-D and 3-D velocity imaging and gives a number of examples. Future challenges and the potentials of parallel acquisition systems for flow imaging are also discussed....

  4. 浅谈医院净化区域智能化系统在工程中的应用%The Applications in the Engineering of the Hospital Purification Region Intelligent Systems

    Institute of Scientific and Technical Information of China (English)

    李静娟; 王晨旭

    2012-01-01

    主要介绍医院净化区域智能化系统的设置,特别是手术部智能化各子系统设置内容,且着重强调了一体化手术室实现的具体功能。%This paper introduces the intelligent system settings in the hospital purification region, especially the intelligent subsystem settings in the Department of operation, and it emphasis on the specific functions for the achieve- ment of the integration of the operating room.

  5. Application Analysis of the Pharmaceutical Purification and Low Temperature Air-conditioning and Refrigeration System%医药净化低温空调制冷系统运行的应用分析

    Institute of Scientific and Technical Information of China (English)

    王巍

    2011-01-01

    This paper introduces the structure and principle of purification and air-conditioning system, and analyzes the cooling and defrosting modes from aspects of technique application and energy saving. Defrosting is the key link of low-temperature purification air-conditioning system. The system mentioned in this paper adopted double air-conditioning boxes. Each box is equipped with separate refrigeration system and is able to cool and defrost alternately, thus to meet the demand of manufacturing technique to the environment.%本文简述了净化制冷空调系统的基本构成与制冷原理.对低温表冷器的供冷与化霜方式,从技术应用和节能方面进行了分析.低温净化空调的制冷系统关键环节是要解决好化霜,通过采用双空调箱,分别各自设有独立的制冷系统,交替制冷或化霜,不问断送风,能解决生产工艺对环境的特殊要求.

  6. Comparison of SAGS I vs. SAGS II delivery systems in emerging implantation technologies

    Science.gov (United States)

    Despres, Joseph; Sweeney, Joseph

    2012-11-01

    The International Fire Code has classified Subatmospheric Gas Delivery Systems (SAGS) technologies into two main categories: SAGS Type I and SAGS Type II systems. SAGS Type I delivery systems both store and deliver gases at subatmospheric pressures. An example of this technology is ATMI's Safe Delivery Source (SDS®) adsorbent based cylinder. SAGS Type II delivery systems store fluids at high pressure and utilize mechanical devices internal to the cylinder to deliver the gas at subatmospheric pressures. Typical mechanical devices used to enable subatmospheric delivery are either set point regulators or mechanical capillary based systems. This paper focuses on how these delivery systems perform against the unique requirements of traditional beam line ion implantation as well as solar and flat panel applications. Specifically, data are provided showing the capability of these systems with respect to flow rate, residual gas left within the cylinder, and cylinder end-point flow and delivery pressure dynamics.

  7. Optimal Multi-type DGs Placement in Primary Distribution System by NSGA-II

    Directory of Open Access Journals (Sweden)

    K. Buayai

    2012-09-01

    Full Text Available The study proposes a multiobjective optimal placement of multi-type DG for enhancement of primary distribution system performance. A Pareto-based non-dominated sorting genetic algorithm II (NSGAII is proposed to determine locations and sizes of specified number of Distributed Generator units (DG within the primary distribution system. Three objective functions are considered as the indexes of the system performance: average Load Voltage Deviation (LVD minimization of the system real power loss and minimization of the annualized investment costs of DG. A fuzzy decision making analysis is used to obtain the final trade off optimal solution. The proposed methodology is tested on modified IEEE 33-bus radial system. Test results indicate that NSGA-II is a viable planning tool for practical DG placement and useful contribution of DG in improving the steady state system performance of the distribution system by the optimal allocation, setting and sizing multi-type DG.

  8. A co-beneficial system using aquatic plants: bioethanol production from free-floating aquatic plants used for water purification.

    Science.gov (United States)

    Soda, S; Mishima, D; Inoue, D; Ike, M

    2013-01-01

    A co-beneficial system using constructed wetlands (CWs) planted with aquatic plants is proposed for bioethanol production and nutrient removal from wastewater. The potential for bioethanol production from aquatic plant biomass was experimentally evaluated. Water hyacinth and water lettuce were selected because of their high growth rates and easy harvestability attributable to their free-floating vegetation form. The alkaline/oxidative pretreatment was selected for improving enzymatic hydrolysis of the aquatic plants. Ethanol was produced with yields of 0.14-0.17 g-ethanol/ g-biomass in a simultaneous saccharification and fermentation mode using a recombinant Escherichia coli strain or a typical yeast strain Saccharomyces cerevisiae. Subsequently, the combined benefits of the CWs planted with the aquatic plants for bioethanol production and nutrient removal were theoretically estimated. For treating domestic wastewater at 1,100 m(3)/d, it was inferred that the anoxic-oxic activated sludge process consumes energy at 3,200 MJ/d, whereas the conventional activated sludge process followed by the CW consumes only 1,800 MJ/d with ethanol production at 115 MJ/d.

  9. Purification and in vitro antioxidative effects of giant squid muscle peptides on free radical-mediated oxidative systems.

    Science.gov (United States)

    Rajapakse, Niranjan; Mendis, Eresha; Byun, Hee-Guk; Kim, Se-Kwon

    2005-09-01

    Low molecular weight peptides obtained from ultrafiltration (UF) of giant squid (Dosidicus gigas) muscle protein were studied for their antioxidative effects in different in vitro oxidative systems. The most potent two peptides, Asn-Ala-Asp-Phe-Gly-Leu-Asn-Gly-Leu-Glu-Gly-Leu-Ala (1307 Da) and Asn-Gly-Leu-Glu-Gly-Leu-Lys (747 Da), exhibited their antioxidant potential to act as chain-breaking antioxidants by inhibiting radical-mediated peroxidation of linoleic acid, and their activities were closer to highly active synthetic antioxidant, butylated hydroxytoluene. Addition of these peptides could enhance the viability of cytotoxic embryonic lung fibroblasts significantly (P<.05) at a low concentration of 50 microg/ml, and it was presumed due to the suppression of radical-induced oxidation of membrane lipids. Electron spin trapping studies revealed that the peptides were potent scavengers of free radicals in the order of carbon-centered (IC(50) 396.04 and 304.67 microM), hydroxyl (IC(50) 497.32 and 428.54 microM) and superoxide radicals (IC(50) 669.34 and 573.83 microM). Even though the exact molecular mechanism for scavenging of free radicals was unclear, unusually high hydrophobic amino acid composition (more than 75%) of giant squid muscle peptides was presumed to be involved in the observed activities.

  10. Power train and emission control: allocation procedure by OBD-II system for automotive technology

    Science.gov (United States)

    Kalita, Porag

    2017-06-01

    OBD-II, systems were designed to maintain low emissions of in use vehicles, including light and medium duty vehicles. In 1989, the California code of Regulations (CCR) known as OBD - II was adopted by the California Air Resource Board (CARB) and the objective to reduce hydrocarbon (HC) emission caused by malfunction of the vehicles emission control systems. OBD-II provides additional information to engineer for diagnosis and repair of emissions related problems. OBD-II, standardizes on the amount of memory (Freeze Frame) it uses to store the readings of the vehicle sensor when it logs on emission related Intermittent Trouble code (IT). The intent of OBD-II, systems is to detect most vehicle malfunctions when performance of a power train component or system deteriorates to the point that the vehicle’s HC emission exceed standard. The vehicle operator is notified at the time when the vehicle begins to marginally exceed emission standards, by illuminating the Malfunctions Indicator Light (MIL).

  11. CARB OBD-II Systems and their impact on alternative fuel vehicles

    Energy Technology Data Exchange (ETDEWEB)

    Baltusis, P. [Ford Motor Co., Dearborn, MI (United States). Powertrain and Materials Research Lab.

    1995-12-31

    An emission control system OBD-II and its impact on vehicle systems and components was discussed. OBD-II is a real time, on-board monitoring system that is part of the power-train control module. It consists of software, sensors and a malfunction indicator light; it monitors all emission related components and systems as the vehicle is being driven. The objectives of OBD-II were to (1) reduce high in-use emissions caused by emission-related malfunctions, (2) reduce time between occurrence of a malfunction and its detection repair, and (3) assist in the diagnosis and repair of emission-related problems. Generally, malfunctions are detected within two driving cycles. The OBD-II monitoring requirements for alternative fuel vehicles such as methanol, flex fuel, ethanol, natural gas, the implementation time table and exceptions thereto, and the unique OBD-II requirements for diesel engines for 1996 (deferrable to 1997 with Executive Officer approval), were also summarized. 15 figs.

  12. Chaotic time series. Part II. System Identification and Prediction

    Directory of Open Access Journals (Sweden)

    Bjørn Lillekjendlie

    1994-10-01

    Full Text Available This paper is the second in a series of two, and describes the current state of the art in modeling and prediction of chaotic time series. Sample data from deterministic non-linear systems may look stochastic when analysed with linear methods. However, the deterministic structure may be uncovered and non-linear models constructed that allow improved prediction. We give the background for such methods from a geometrical point of view, and briefly describe the following types of methods: global polynomials, local polynomials, multilayer perceptrons and semi-local methods including radial basis functions. Some illustrative examples from known chaotic systems are presented, emphasising the increase in prediction error with time. We compare some of the algorithms with respect to prediction accuracy and storage requirements, and list applications of these methods to real data from widely different areas.

  13. Continuous and distributed systems II theory and applications

    CERN Document Server

    Zgurovsky, Mikhail

    2015-01-01

    As in the previous volume on the topic, the authors close the gap between abstract mathematical approaches, such as applied methods of modern algebra and analysis, fundamental and computational mechanics, nonautonomous and stochastic dynamical systems, on the one hand, and practical applications in nonlinear mechanics, optimization, decision making theory and control theory on the other. Readers will also benefit from the presentation of modern mathematical modeling methods for the numerical solution of complicated engineering problems in biochemistry, geophysics, biology and climatology. This compilation will be of interest to mathematicians and engineers working at the interface of these fields. It presents selected works of the joint seminar series of Lomonosov Moscow State University and the Institute for Applied System Analysis at National Technical University of Ukraine “Kyiv Polytechnic Institute”. The authors come from Brazil, Germany, France, Mexico, Spain, Poland, Russia, Ukraine, and the USA. ...

  14. New Solvents for Cellulose. II. Ethylenediamine/Thiocyanate Salt System

    OpenAIRE

    HATTORI, Kazuyuki; ABE, Emiko; Yoshida, Takashi; CUCULO, John A.; 服部,和幸; 吉田, 孝

    2004-01-01

    The ethylenediamine/thiocyanate salt system was found to be a new solvent for cellulose. The solubility, dissolution behavior, solution properties, and cellulose recovered from the solutions were investigated. The dissolution took place at room temperature, and the maximum solubility achieved was 16% (w/w) for cellulose of DP210 in the ethylenediamine/sodium thiocyanate 54/46 (w/w).The dependence of cellulose solubility on DP is also described. Tracing the dissolution behavior of the cellulos...

  15. The Chimera II Real-Time Operating System for advanced sensor-based control applications

    Science.gov (United States)

    Stewart, David B.; Schmitz, Donald E.; Khosla, Pradeep K.

    1992-01-01

    Attention is given to the Chimera II Real-Time Operating System, which has been developed for advanced sensor-based control applications. The Chimera II provides a high-performance real-time kernel and a variety of IPC features. The hardware platform required to run Chimera II consists of commercially available hardware, and allows custom hardware to be easily integrated. The design allows it to be used with almost any type of VMEbus-based processors and devices. It allows radially differing hardware to be programmed using a common system, thus providing a first and necessary step towards the standardization of reconfigurable systems that results in a reduction of development time and cost.

  16. Planetary Data Systems (PDS) Imaging Node Atlas II

    Science.gov (United States)

    Stanboli, Alice; McAuley, James M.

    2013-01-01

    The Planetary Image Atlas (PIA) is a Rich Internet Application (RIA) that serves planetary imaging data to the science community and the general public. PIA also utilizes the USGS Unified Planetary Coordinate system (UPC) and the on-Mars map server. The Atlas was designed to provide the ability to search and filter through greater than 8 million planetary image files. This software is a three-tier Web application that contains a search engine backend (MySQL, JAVA), Web service interface (SOAP) between server and client, and a GWT Google Maps API client front end. This application allows for the search, retrieval, and download of planetary images and associated meta-data from the following missions: 2001 Mars Odyssey, Cassini, Galileo, LCROSS, Lunar Reconnaissance Orbiter, Mars Exploration Rover, Mars Express, Magellan, Mars Global Surveyor, Mars Pathfinder, Mars Reconnaissance Orbiter, MESSENGER, Phoe nix, Viking Lander, Viking Orbiter, and Voyager. The Atlas utilizes the UPC to translate mission-specific coordinate systems into a unified coordinate system, allowing the end user to query across missions of similar targets. If desired, the end user can also use a mission-specific view of the Atlas. The mission-specific views rely on the same code base. This application is a major improvement over the initial version of the Planetary Image Atlas. It is a multi-mission search engine. This tool includes both basic and advanced search capabilities, providing a product search tool to interrogate the collection of planetary images. This tool lets the end user query information about each image, and ignores the data that the user has no interest in. Users can reduce the number of images to look at by defining an area of interest with latitude and longitude ranges.

  17. NMR System for a Type II Quantum Computer

    Science.gov (United States)

    2007-06-01

    ia/h s -ic/h -ib/•h -ic/h s The inversion of a general 3 x 3 matrix is known to be d- im-jl fl-em ej-fi h iij-mdm-fk fh-djejk+,fh +dim-fik-djl- ehm [k...subjected to a strong magnetic field B0 of order , 10 T creating an energy difference AE between the aligned and anti-aligned spin states that results in...and the magnetic field B0 , are the resonant frequencies to flip the spins. The internal Hamniltonian of this system in a strong and homogeneous

  18. Saenger II, a hypersonic flight and space transportation system

    Science.gov (United States)

    Koelle, Dietrich E.

    The paper presents the actual design status of the Saenger advanced space transportation system which comprises a hypersonic aircraft as first stage (EHTV). This vehicle (European Hypersonic Transport Vehicle) has been conceived for a dual purpose: to serve as the first stage of a launch vehicle with cruise capability, which is required to reach the space station orbit (28.5 deg) from Europe, and in the same basic configuration as passenger plane with some 230 passengers for a range of more than 10,000 km. The optimum cruise speed seems to be Mach 4.4 in 24.5 km altitude for economic and environmental reasons.

  19. Thiol redox requirements and substrate specificities of recombinant cytochrome c assembly systems II and III.

    Science.gov (United States)

    Richard-Fogal, Cynthia L; San Francisco, Brian; Frawley, Elaine R; Kranz, Robert G

    2012-06-01

    The reconstitution of biosynthetic pathways from heterologous hosts can help define the minimal genetic requirements for pathway function and facilitate detailed mechanistic studies. Each of the three pathways for the assembly of cytochrome c in nature (called systems I, II, and III) has been shown to function recombinantly in Escherichia coli, covalently attaching heme to the cysteine residues of a CXXCH motif of a c-type cytochrome. However, recombinant systems I (CcmABCDEFGH) and II (CcsBA) function in the E. coli periplasm, while recombinant system III (CCHL) attaches heme to its cognate receptor in the cytoplasm of E. coli, which makes direct comparisons between the three systems difficult. Here we show that the human CCHL (with a secretion signal) attaches heme to the human cytochrome c (with a signal sequence) in the E. coli periplasm, which is bioenergetically (p-side) analogous to the mitochondrial intermembrane space. The human CCHL is specific for the human cytochrome c, whereas recombinant system II can attach heme to multiple non-cognate c-type cytochromes (possessing the CXXCH motif.) We also show that the recombinant periplasmic systems II and III use components of the natural E. coli periplasmic DsbC/DsbD thiol-reduction pathway. This article is part of a Special Issue entitled: Biogenesis/Assembly of Respiratory Enzyme Complexes.

  20. Data acquisition system based on the Nios II for a CCD camera

    Science.gov (United States)

    Li, Binhua; Hu, Keliang; Wang, Chunrong; Liu, Yangbing; He, Chun

    2006-06-01

    The FPGA with Avalon Bus architecture and Nios soft-core processor developed by Altera Corporation is an advanced embedded solution for control and interface systems. A CCD data acquisition system with an Ethernet terminal port based on the TCP/IP protocol is implemented in NAOC, which is composed of a piece of interface board with an Altera's FPGA, 32MB SDRAM and some other accessory devices integrated on it, and two packages of control software used in the Nios II embedded processor and the remote host PC respectively. The system is used to replace a 7200 series image acquisition card which is inserted in a control and data acquisition PC, and to download commands to an existing CCD camera and collect image data from the camera to the PC. The embedded chip in the system is a Cyclone FPGA with a configurable Nios II soft-core processor. Hardware structure of the system, configuration for the embedded soft-core processor, and peripherals of the processor in the PFGA are described. The C program run in the Nios II embedded system is built in the Nios II IDE kits and the C++ program used in the PC is developed in the Microsoft's Visual C++ environment. Some key techniques in design and implementation of the C and VC++ programs are presented, including the downloading of the camera commands, initialization of the camera, DMA control, TCP/IP communication and UDP data uploading.

  1. Traveling wavefronts in nonlocal diffusive predator-prey system with Holling type II functional response

    Directory of Open Access Journals (Sweden)

    Shuang Li

    2015-06-01

    Full Text Available This article concerns the existence of traveling wavefronts for a nonlocal diffusive predator-prey system with functional response of Holling type II. We first establish the existence principle for the system with a general functional response by using a fixed point theorem and upper-lower solution technique. We apply this result to a predator-prey model with Holling type II functional response. We deduce the existence of traveling wavefronts that connect the zero equilibrium and the positive equilibrium.

  2. Removal of Pb, Cd, and Cr in a water purification system using modified mineral waste materials and activated carbon derived from waste materials

    Science.gov (United States)

    Lu, H. R.; Su, L. C.; Ruan, H. D.

    2016-08-01

    This study attempts to find out and optimize the removal efficiency of heavy metals in a water purification unit using a low-cost waste material and modified mineral waste materials (MMWM) accompanied with activated carbon (AC) derived from waste materials. The factors of the inner diameter of the purification unit (2.6-5cm), the height of the packing materials (5-20cm), the size of AC (200-20mesh), the size of MMWM (1-0.045mm), and the ratio between AC and MMWM in the packing materials (1:0 - 0:1) were examined based on a L18 (5) 3 orthogonal array design. In order to achieve an optimally maximum removal efficiency, the factors of the inner diameter of the purification unit (2.6-7.5cm), the height of the packing materials (10-30cm), and the ratio between AC and MMWM in the packing materials (1:4-4:1) were examined based on a L16 (4) 3 orthogonal array design. A height of 25cm, inner diameter of 5cm, ratio between AC and MMWM of 3:2 with size of 60-40mesh and 0.075-0.045mm, respectively, were the best conditions determined by the ICP-OES analysis to perform the adsorption of heavy metals in this study.

  3. 25 CFR 547.12 - What are the minimum technical standards for downloading on a Class II gaming system?

    Science.gov (United States)

    2010-04-01

    ... on a Class II gaming system? 547.12 Section 547.12 Indians NATIONAL INDIAN GAMING COMMISSION... OF CLASS II GAMES § 547.12 What are the minimum technical standards for downloading on a Class II... modification, in accordance with § 547.15(a). (4) Downloads conducted during operational periods shall be...

  4. 25 CFR 547.8 - What are the minimum technical software standards applicable to Class II gaming systems?

    Science.gov (United States)

    2010-04-01

    ... applicable to Class II gaming systems? 547.8 Section 547.8 Indians NATIONAL INDIAN GAMING COMMISSION... OF CLASS II GAMES § 547.8 What are the minimum technical software standards applicable to Class II... game provided to players pursuant to § 547.16. Any change in rules constitutes a different game. There...

  5. Improvement of the following accident dose assessment system (II)

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Enn Han; Han, Moon Hee; Suh, Kyung Suk; Hwang, Won Tae; Choi, Young Gil [Korea Atomic Energy Research Institute, Taejeon (Korea)

    2000-12-01

    The FADAS and its database have been updates for calculating the real-time wind fields continuously at the nuclear sites in Korea. The system has been constructed to compute the wind fields using its own process for the dummy meteorological data, and does not effect on the overall wind field module. If the radioactive materials are released into the atmosphere in real situation, the calculations of wind fields and exposure dose in the previous FADAS are performed in the case of the recognition of the above situation in the source term evaluation module. The current version of FADAS includes the program for evaluating the effect of the predicted accident and the assumed scenario together. The dose assessment module is separated into the real-time and the supposed accident respectively. 7 refs., 8 figs., 6 tabs. (Author)

  6. Intelligent systems II complete approximation by neural network operators

    CERN Document Server

    Anastassiou, George A

    2016-01-01

    This monograph is the continuation and completion of the monograph, “Intelligent Systems: Approximation by Artificial Neural Networks” written by the same author and published 2011 by Springer. The book you hold in hand presents the complete recent and original work of the author in approximation by neural networks. Chapters are written in a self-contained style and can be read independently. Advanced courses and seminars can be taught out of this brief book. All necessary background and motivations are given per chapter. A related list of references is given also per chapter. The book’s results are expected to find applications in many areas of applied mathematics, computer science and engineering. As such this monograph is suitable for researchers, graduate students, and seminars of the above subjects, also for all science and engineering libraries.  .

  7. Diagnosing and curing system freeze-ups: Part II

    Energy Technology Data Exchange (ETDEWEB)

    Frey, R. [Heating and Plumbing Service, Sparta, NJ (United States)

    1996-02-01

    In our last article, we discussed determination and possible causes leading to frozen pipes in the heating system. We now turn to the matter of dealing with such freeze-ups. There are two major categories of frozen heating systems. The first and worst we can label the {open_quotes}Floridian Flew{close_quotes} type, when the usual occupants have taken off for warmer climates. The second is the {open_quotes}Howcudit {open_quote}B{close_quote} virus,{close_quotes} which occurs to normal people under an unusual Arctic Attack right while they are living there. Regarding the first, I never cease to be amazed how folks can just up and leave their house for three days to three months without making proper arrangements for monitoring the temperature of their home during their absence. There are a frightening number of humanoids out there, who either lack a minimal presence of common sense, or who are terrible gamblers. The amazing thing is that some of these folks wouldn`t even gamble a postage stamp on a magazine sweepstakes, but still will gamble the contents of their home by driving off for two weeks at Disneyland in the middle of January without ever making any provision for assuring that their house doesn`t freeze up while they are away. The {open_quotes}Howcudit {open_quote}B{close_quote}{close_quotes} type is not nearly as devasting as the {open_quotes}Floridian Flew.{close_quotes} Imagine coming home to an icy cold house after flying in from the sunny beaches of some tropical paradise. The oilburner switch is on. The thermostate is set at 60 where we left it, but the needle is buried somewhere down behind the cover, like it is cowering from guilt. {open_quotes}Oh Man! I just checked the bathroom; there is an icicle hanging from the vanity faucet and the toilet bowl is a block of ice.{close_quotes}

  8. A Photo Storm Report Mobile Application, Processing/Distribution System, and AWIPS-II Display Concept

    Science.gov (United States)

    Longmore, S. P.; Bikos, D.; Szoke, E.; Miller, S. D.; Brummer, R.; Lindsey, D. T.; Hillger, D.

    2014-12-01

    The increasing use of mobile phones equipped with digital cameras and the ability to post images and information to the Internet in real-time has significantly improved the ability to report events almost instantaneously. In the context of severe weather reports, a representative digital image conveys significantly more information than a simple text or phone relayed report to a weather forecaster issuing severe weather warnings. It also allows the forecaster to reasonably discern the validity and quality of a storm report. Posting geo-located, time stamped storm report photographs utilizing a mobile phone application to NWS social media weather forecast office pages has generated recent positive feedback from forecasters. Building upon this feedback, this discussion advances the concept, development, and implementation of a formalized Photo Storm Report (PSR) mobile application, processing and distribution system and Advanced Weather Interactive Processing System II (AWIPS-II) plug-in display software.The PSR system would be composed of three core components: i) a mobile phone application, ii) a processing and distribution software and hardware system, and iii) AWIPS-II data, exchange and visualization plug-in software. i) The mobile phone application would allow web-registered users to send geo-location, view direction, and time stamped PSRs along with severe weather type and comments to the processing and distribution servers. ii) The servers would receive PSRs, convert images and information to NWS network bandwidth manageable sizes in an AWIPS-II data format, distribute them on the NWS data communications network, and archive the original PSRs for possible future research datasets. iii) The AWIPS-II data and exchange plug-ins would archive PSRs, and the visualization plug-in would display PSR locations, times and directions by hour, similar to surface observations. Hovering on individual PSRs would reveal photo thumbnails and clicking on them would display the

  9. Optical payload isolation using the Miniature Vibration Isolation System (MVIS-II)

    Science.gov (United States)

    McMickell, M. B.; Kreider, Thom; Hansen, Eric; Davis, Torey; Gonzalez, Mario

    2007-04-01

    Precision satellite payloads commonly require isolation from bus disturbance sources, such as reaction wheels, thrusters, stepper motors, cryo-coolers, solar array drives, thermal popping, and other moving devices. Since nearly every satellite essentially has a unique construction, custom isolation systems are usually designed to attenuate a wide bandwidth of disturbance frequencies. The disadvantage of these custom solutions is that they are not easily reusable or transferable and are generally not robust to changes in payload geometry and mass properties during the development. The MVIS-II isolation system is designed to provide vibration disturbance attenuation over a wide bandwidth, as well as being able to adapt to changes in payload mass properties and geometry, through active control of a smart material. MVIS-II is a collaborative effort between the Air Force Research Laboratory (AFRL) Space Vehicle Directorate and Honeywell Defense and Space to validate miniature hybrid (passive/active) vibration isolation of sensitive optical payloads. The original flight experiment was intended to isolate a non-critical representative payload mass for demonstration purposes; however, the MVIS-II has been adapted to support the primary optical payload onboard the Tactical Satellite 2 (TacSat-2). Throughout the program MVIS-II has been able to adapt to changes in the payload geometry and mass properties with modification limited to support structures only. The MVIS-II system consists of a hexapod of hybrid struts, where each strut includes a patented passive 3-parameter DStrut n series with a novel hydraulically amplified piezoelectric actuator with integral load cell. Additionally, Honeywell's Flexible I/O controller electronics and software are used for command and control of the hardware. The passive D-Strut element provides a 40 dB/decade passive roll-off to attenuate mid-to-high frequency disturbances, while the active piezoelectric actuator is used for enhanced low

  10. High average power CO II laser MOPA system for Tin target LPP EUV light source

    Science.gov (United States)

    Ariga, Tatsuya; Hoshino, Hideo; Endo, Akira

    2007-02-01

    Extreme ultraviolet lithography (EUVL) is the candidate for next generation lithography to be introduced by the semiconductor industry to HVM (high volume manufacturing) in 2013. The power of the EUVL light source has to be at least 115W at a wavelength of 13.5nm. A laser produced plasma (LPP) is the main candidate for this light source but a cost effective laser driver is the key requirement for the realization of this concept. We are currently developing a high power and high repetition rate CO II laser system to achieve 50 W intermediate focus EUV power with a Tin droplet target. We have achieved CE of 2.8% with solid Tin wire target by a transversely excited atmospheric (TEA) CO II laser MOPA system with pulse width, pulse energy and pulse repetition rate as 10~15 ns, 30 mJ and 10 Hz, respectively. A CO II laser system with a short pulse length less than 15 ns, a nominal average power of a few kW, and a repetition rate of 100 kHz, based on RF-excited, fast axial flow CO II laser amplifiers is under development. Output power of about 3 kW has been achieved with a pulse length of 15 ns at 130 kHz repletion rate in a small signal amplification condition with P(20) single line. The phase distortion of the laser beam after amplification is negligible and the beam can be focused to about 150μm diameter in 1/e2. The CO II laser system is reported on short pulse amplification performance using RF-excited fast axial flow lasers as amplifiers. And the CO II laser average output power scaling is shown towards 5~10 kW with pulse width of 15 ns from a MOPA system.

  11. 血液净化专科护士胜任力指标体系的构建%Construction of competency index system of blood purification specialist nurses

    Institute of Scientific and Technical Information of China (English)

    刘晓鑫; 李乐之

    2016-01-01

    Objective:To construct a competency index system for blood purification specialist nurses and to pro-vide basis for selection,training,performance evaluation,management of specialist nurses.Methods:Semi struc-tured depth interview method and critical incident method were adopted to interview 12 blood purification ex-perts and 12 blood purification specialist nurses in Hunan province,All the data were collected and analyzed by NVivo QSR 8 Chinese version software.Results:Competency index system for blood purification specialist nur-ses included professional knowledge,professional skills,professional ability,personality and self concept.And the 5 dimensions were divided into 18 items.There were some differences in the cognition of the competency of nurses in different position of blood purification.Conclusions:When selecting and training of blood purification specialist nurses,in addition to consider their professional knowledge and skills,one should also pay attention to test and develop their teaching ability,service consciousness,protection,inner concentration spirit,empathy and dedication spirit,and so on,so as to achieve a comprehensive and scientific evaluation.%[目的]构建血液净化专科护士胜任力指标体系,为专科护士选拔、培训、绩效考核、管理等提供依据。[方法]采用半结构式深度访谈法、关键事件法对湖南省12名血液净化专家和12名血液净化专科护士进行访谈收集资料,资料分析采用 QSR NVivo 8.0中文版软件。[结果]血液净化专科护士胜任指标体系包含专业知识、专业技术、专业能力、人格特质及自我概念5个维度(18个项目);不同岗位受访者对血液净化专科护士胜任力特征的认知存在一定差异。[结论]在选聘及培训血液净化专科护士时,除其专业知识技能方面外,还应注重考查和培养其服务意识、防护能力、慎独精神、同理心、敬业精神等,以达到全面、科学地测评。

  12. [Extraction and purification method of rice DNA from rice powder containing Konjak flour].

    Science.gov (United States)

    Minematsu, Kazuhiko; Nakamura, Kosuke; Akiyama, Hiroshi; Harikai, Naoki; Nakajima, Osamu; Kitta, Kazumi; Teshima, Reiko; Iizuka, Tayoshi

    2010-01-01

    Rice powder containing Konjak flour made with tuberous roots of Amorphophallus konjac is imported as a rice-processed product from China to Japan. An improved DNA purification method for the polymerase chain reaction (PCR) analysis of rice in such products is necessary, since Konjak flour constituents absorb the DNA purification buffer to form a gel, and cause problems in the subsequent purification steps. Here, we present a simple preparative system for isolation of the rice and a purification method of the rice DNA from the product. The purified DNA was confirmed to be a good template for both PCR and real-time PCR.

  13. A New Four-Barrel Pellet Injection System for the TJ-II Stellarator

    Energy Technology Data Exchange (ETDEWEB)

    Combs, Stephen Kirk [ORNL; Foust, Charles R [ORNL; McGill, James M [ORNL; Baylor, Larry R [ORNL; Caughman, John B [ORNL; Fehling, Dan T [ORNL; Harris, Jeffrey H [ORNL; Meitner, Steven J [ORNL; Rasmussen, David A [ORNL; McCarthy, K. J. [EURATOM-CIEMAT, Madrid, Spain; Chamorro, M. [Laboratory Nacional de Fusion, Madrid, Spain; Garcia, R. [Laboratory Nacional de Fusion, Madrid, Spain; Hildago, C. [Laboratory Nacional de Fusion, Madrid, Spain; Medrano, M. [Laboratory Nacional de Fusion, Madrid, Spain; Unamuno, R. [Laboratory Nacional de Fusion, Madrid, Spain

    2011-01-01

    A new pellet injection system for the TJ-II stellarator has been developed/constructed as part of a collaboration between the Oak Ridge National Laboratory (ORNL) and the Centro de Investigaciones Energ ticas, Medioambientales y Tecnol gicas (CIEMAT). ORNL is providing most of the injector hardware and instrumentation, the pellet diagnostics, and the pellet transport tubes; CIEMAT is responsible for the injector stand/interface to the stellarator, cryogenic refrigerator, vacuum pumps/ballast volumes, gas manifolds, remote operations, plasma diagnostics, and data acquisition. The pellet injector design is an upgraded version of that used for the ORNL injector installed on the Madison Symmetric Torus (MST). It is a four-barrel system equipped with a cryogenic refrigerator for in situ hydrogen pellet formation and a combined mechanical punch/propellant valve system for pellet acceleration (speeds ~100 to 1000 m/s). On TJ-II, it will be used as an active diagnostic and for fueling. To accommodate the plasma experiments planned for TJ-II, pellet sizes significantly smaller than those typically used for the MST application are required. The system will initially be equipped with four different pellet sizes, with the gun barrel bores ranging between ~0.5 to 1.0 mm. The new system is almost complete and is described briefly here, highlighting the new features added since the original MST injector was constructed. Also, the future installation on TJ-II is reviewed.

  14. Introduction to Integrable Many-Body Systems II

    Science.gov (United States)

    Šamaj, Ladislav

    2010-04-01

    This is the second part of a three-volume introductory course about integrable systems of interacting bodies. The models of interest are quantum spin chains with nearest-neighbor interactions between spin operators, in particular Heisenberg spin-1/2 models. The Ising model in a transverse field, expressible as a quadratic fermion form by using the Jordan-Wigner transformation, is the subject of Sect. 12. The derivation of the coordinate Bethe ansatz for the XXZ Heisenberg chain and the determination of its absolute ground state in various regions of the anisotropy parameter are presented in Sect. 13. The magnetic properties of the ground state are explained in Sect. 14. Sect. 15 concerns excited states and the zero-temperature thermodynamics of the XXZ model. The thermodynamics of the XXZ Heisenberg chain is derived on the basis of the string hypothesis in Sect. 16; the thermodynamic Bethe ansatz equations are analyzed in high-temperature and low-temperature limits. An alternative derivation of the thermodynamics without using strings, leading to a non-linear integral equation determining the free energy, is the subject of Sect. 17. A nontrivial application of the Quantum Inverse Scattering method to the fully anisotropic XYZ Heisenberg chain is described in Sect. 18. Sect. 19 deals with integrable cases of isotropic spin chains with an arbitrary spin.

  15. Models of cuspy triaxial stellar systems. II. Regular orbits

    CERN Document Server

    Muzzio, J C; Zorzi, A F

    2012-01-01

    In the first paper of this series we used the N--body method to build a dozen cuspy (gamma ~ 1) triaxial models of stellar systems, and we showed that they were highly stable over time intervals of the order of a Hubble time, even though they had very large fractions of chaotic orbits (more than 85 per cent in some cases). The models were grouped in four sets, each one comprising models morphologically resembling E2, E3, E4 and E5 galaxies, respectively. The three models within each set, although different, had the same global properties and were statistically equivalent. In the present paper we use frequency analysis to classify the regular orbits of those models. The bulk of those orbits are short axis tubes (SATs), with a significant fraction of long axis tubes (LATs) in the E2 models that decreases in the E3 and E4 models to become negligibly small in the E5 models. Most of the LATs in the E2 and E3 models are outer LATs, but the situation reverses in the E4 and E5 models where the few LATs are mainly inn...

  16. Recorded seismic response of Pacific Park Plaza. II. System identification

    Science.gov (United States)

    Safak, F.; Celebi, M.

    1992-01-01

    This is the second of two companion papers on the recorded seismic response of the Pacific Park Plaza building, in Emeryville, Calif., during the October 17, 1989, Ms = 7.1 (surface-wave magnitude) Loma Prieta earthquake. In this second part, the recorded data are analyzed in more detail by using system-identification techniques. The three-dimensional behavior and the coupled modes of the building are determined, and the effects of soil-structure interaction are investigated. The study shows that the response of the building is nonlinear at the beginning, and becomes linear after 17 sec into the earthquake. The dominant motion of the building follows an elliptical path oriented in the southeast-northwest direction. Some of the modes are complex, with nonproportional damping, and there are phase differences among modal response components. The fundamental mode of the building is a translation in the southeast-northwest direction at 0.4 Hz, with 13% damping. The wing displacements relative to the center core are large, about 50% of the center core displacements, and indicate significant torsion in the center core. The soil-structure interaction is characterized by a vibration at 0.7 Hz. This is believed to be the fundamental frequency of the surrounding soil medium. The rocking motions of the building are negligible.

  17. Fates of satellite ejecta in the Saturn system, II

    Science.gov (United States)

    Alvarellos, José Luis; Dobrovolskis, Anthony R.; Zahnle, Kevin J.; Hamill, Patrick; Dones, Luke; Robbins, Stuart

    2017-03-01

    We assess the fates of ejecta from the large craters Aeneas on Dione and Ali Baba on Enceladus (161 and 39 km in diameter, respectively), as well as that from Herschel (130 km in diameter) on Mimas. The ejecta are treated either as 'spalls' launched from hard surfaces, or as 'rubble' launched from a weak rubble pile regolith. Once in orbit we consider the ejecta as massless test particles subject to the gravity of Saturn and its classical satellites. The great majority of escaped ejecta get swept up by the source moons. The best fit to the ejecta population decay is a stretched exponential with exponent near 1/2 (Dobrovolskis et al., Icarus 188, 481-505, 2007). We bracket the characteristic ejecta sizes corresponding to Grady-Kipp fragments and spalls. Based on this and computed impact velocities and incidence angles, the resulting sesquinary craters, if they exist, should have diameters on the order of a few meters to a few km. The observed longitude distribution of small craters on Mimas along with the findings of Bierhaus et al. that small moons should not have a secondary crater population (Icarus 218, 602-621, 2012) suggest that the most likely place to find sesquinary craters in the Saturn system is the antapex of Mimas.

  18. Mars Hybrid Propulsion System Trajectory Analysis. Part II; Cargo Missions

    Science.gov (United States)

    Chai, Patrick R.; Merrill, Raymond G.; Qu, Min

    2015-01-01

    NASA's Human Spaceflight Architecture Team is developing a reusable hybrid transportation architecture in which both chemical and electric propulsion systems are used to send crew and cargo to Mars destinations such as Phobos, Deimos, the surface of Mars, and other orbits around Mars. By combining chemical and electrical propulsion into a single spaceship and applying each where it is more effective, the hybrid architecture enables a series of Mars trajectories that are more fuel-efficient than an all chemical architecture without significant increases in flight times. This paper shows the feasibility of the hybrid transportation architecture to pre-deploy cargo to Mars and Phobos in support of the Evolvable Mars Campaign crew missions. The analysis shows that the hybrid propulsion stage is able to deliver all of the current manifested payload to Phobos and Mars through the first three crew missions. The conjunction class trajectory also allows the hybrid propulsion stage to return to Earth in a timely fashion so it can be reused for additional cargo deployment. The 1,100 days total trip time allows the hybrid propulsion stage to deliver cargo to Mars every other Earth-Mars transit opportunity. For the first two Mars surface mission in the Evolvable Mars Campaign, the short trip time allows the hybrid propulsion stage to be reused for three round-trip journeys to Mars, which matches the hybrid propulsion stage's designed lifetime for three round-trip crew missions to the Martian sphere of influence.

  19. Electron Recirculation in Electrostatic Multicusp Systems: II - System Performance Scaling of One-Dimensional ’Rollover’ Wells

    Science.gov (United States)

    1992-01-01

    GCUSPII.T3;vers 3; 07/31/92> AMA C D-A257 942 Electron Recirculation In Electrostatic Multicusp Systems: II - System Performance Scaling Of One...1 * criteria necessary for effective electron confinement in Polywelltf--type multicusp systems. These special polyhedral configurations 7. s allow...anisotropic radial energy in both species, in multicusp Polywelltm geometry. The bulk problem is treated as one-dimensional, with arbitrary spatial

  20. [Clinical usefulness of serum PIVKA-II levels determined by ECLIA system as a tumor maker for hepatocellular carcinoma].

    Science.gov (United States)

    Sakizono, K; Oita, T; Shibata, Y; Tamura, A; Kasakura, S

    1998-09-01

    PIVKA-II is well known as a tumor maker of hepatocellular carcinoma (HCC). We measured serum PIVKA-II concentrations with a commercially available PIVKA-II immunoassay kit (Picolumi PIVKA-II: Eisai Co., Ltd., Tokyo) using Electrochemiluminescence Immunoassay (ECLIA). ECLIA system is a novel immunoassay system using a Ruthenium (II) Tris (bipyridyl) luminesced by electric energy. Cut off value was 40 mAU/ml by receiver-operating characteristic curves as a tumor maker for HCC. Eighty-nine out of 142 (62.2%) patients with HCC had elevated serum PIVKA-II levels and seventeen out of 36 (47.2%) patients whose tumor size was below 2 cm in diameter showed high PIVKA-II levels. We determined the serial changes in serum PIVKA-II levels of two patients with HCC following initial therapy. In these patients, elevations of serum PIVKA-II levels determined by ECLIA system preceded the HCC relapse detected by imaging diagnostic procedures. In summary, this assay system is suitable for detecting small increases in PIVKA-II concentrations. Determination of PIVKA-II by this assay system is found to be useful for the early detection of HCC.

  1. Purification of GST-Tagged Proteins.

    Science.gov (United States)

    Schäfer, Frank; Seip, Nicole; Maertens, Barbara; Block, Helena; Kubicek, Jan

    2015-01-01

    This protocol describes the purification of recombinant proteins fused to glutathione S-transferase (GST, GST-tagged proteins) by Glutathione Affinity purification. The GST tag frequently increases the solubility of the fused protein of interest and thus enables its purification and subsequent functional characterization. The GST-tagged protein specifically binds to glutathione immobilized to a matrix (e.g., agarose) and can be easily separated from a cell lysate by a bind-wash-elute procedure. GST-tagged proteins are often used to study protein-protein interactions, again making use of glutathione affinity in a procedure called a GST pull-down assay. The protocol is designed to process 200 ml of E. coli culture expressing intermediate to high amounts of a GST-tagged protein (~25 mg l(-1)). Depending on the expression rate or the available culture volume, the scale can be increased or decreased linearly. The protocol can also be used to purify GST-tagged proteins from other expression systems, such as insect or mammalian cells. Tips are provided to aid in modifying certain steps if proteins shall be recovered from alternative expression systems.

  2. Purification of a-galactosidase from seeds of Sesbania marginata

    Directory of Open Access Journals (Sweden)

    Falco A.L.P.

    2000-01-01

    Full Text Available Alpha-galactosidase taken from a raw extract of Sesbania marginata legume seeds was purified by partitioning in aqueous two-phase systems (ATPS. Initially, galactomannan/dextran 2,000,000 systems were used for the purification, and the partition coefficients of alpha -galactosidase varied from 1.5 to 4.0. However, mass transport in these systems was poor due to the high viscosity of the employed polymers. Therefore, partitioning in polyethyleneglycol (PEG/ sodium phosphate systems and the effect of sodium chloride upon the enzyme purification and the yield of alpha -galactosidase were also investigated. The purification achieved in a single-step was 5.7 with a recovery of 144% of alpha -galactosidase, possibly due to the removal of materials which inhibited alpha -galactosidase activity before the purification. The removal of the main protein contaminants and the highest yields were achieved in PEG 4,000/ sodium phosphate + 6% NaCl system at pH 5.0. Further purification by preparative on-exchange chromatography was also developed.

  3. Copper(II) ions interactions in the systems with triamines and ATP. Potentiometric and spectroscopic studies.

    Science.gov (United States)

    Hoffmann, S K; Goslar, J; Bregier-Jarzebowska, R; Gasowska, A; Zalewska, A; Lomozik, L

    2017-09-09

    The mode of interaction and thermodynamic stability of complexes formed in binary and ternary Cu(II)/ATP/triamines systems were studied using potentiometric and spectroscopic (NMR, EPR, UV-Vis) methods. It was found that in binary metal-free systems ATP/HxPA species are formed (PA: Spd=spermidine or 3,3-tri=1,7-diamino-4-azaheptane) where the phosphate groups from nucleotides are preferred negative centers and protonated amine groups of amines are positive centers of reaction. In the ternary systems Cu/ATP/Hx(PA) as well as Cu/(ATP)(PA) species are formed. The type of the formed Cu(II) complexes depends on pH of the solution. For a low pH value the complexation appears between Cu(II) and ATP molecules via oxygen atoms of phosphate groups. For a very high pH value, where ATP is hydrolyzed, the Cu(II) ions are bound to the nitrogen atoms of polyamine molecules. We did not detect any direct coordination of the N7 nitrogen atom of adenosine to Cu(II) ions. It means that the CuN7 interaction is an indirect type and can be due to noncovalent interplay including water molecule. EPR studies were performed at glassy state (77K) after a fast freezing both for binary and ternary systems. The glassy state EPR spectra do not reflect species identified in titration studies indicating significant effect of rapid temperature decrease on equilibrium of Cu(II) complexes. We propose the molecular structure of all the studied complexes at the glassy state deduced from EPR and optical spectroscopy results. Copyright © 2017 Elsevier Inc. All rights reserved.

  4. Logic control of molecular sieve purification system of 65000m3· h-1 air separation plant%65000m3·h-1空分设备分子筛纯化系统逻辑控制

    Institute of Scientific and Technical Information of China (English)

    朱玉芹

    2012-01-01

    The structure and technical process of molecular sieve purification system of 65000m3/ an air separation plant of Hebi coal and electeicity 600 ktpa methanol project are briefed, automatic valve closing / opening logical control of molecular sieve purification system, automatic / manual operation sequence control program,implementation of the sequence control program and optimized reform of the sequence control configuration program of the molecular sieve absorber in accordance with site operation conditions are described.%简介鹤壁煤电股份有限公司化工分公司空分厂65000m3·h-1空分设备分子筛纯化系统结构和工艺流程,介绍分子筛纯化系统阀门自动开关控制逻辑、顺控程序自动/手动运行、顺控程序执行过程,以及根据现场生产运行实际情况,对分子筛吸附器顺控组态程序进行的优化改进.

  5. Calibration of the Accuscan II In Vivo System for I-125 Thyroid Counting

    Energy Technology Data Exchange (ETDEWEB)

    Ovard R. Perry; David L. Georgeson

    2011-07-01

    This report describes the March 2011 calibration of the Accuscan II HpGe In Vivo system for I-125 thyroid counting. The source used for the calibration was a DOE manufactured Am-241/Eu-152 source contained in a 22 ml vial BEA Am-241/Eu-152 RMC II-1 with energies from 26 keV to 344 keV. The center of the detector housing was positioned 64 inches from the vault floor. This position places the approximate center line of the detector housing at the center line of the source in the phantom thyroid tube. The energy and efficiency calibration were performed using an RMC II phantom (Appendix J). Performance testing was conducted using source BEA Am-241/Eu-152 RMC II-1 and Validation testing was performed using an I-125 source in a 30 ml vial (I-125 BEA Thyroid 002) and an ANSI N44.3 phantom (Appendix I). This report includes an overview introduction and records for the energy/FWHM and efficiency calibration including performance verification and validation counting. The Accuscan II system was successfully calibrated for counting the thyroid for I-125 and verified in accordance with ANSI/HPS N13.30-1996 criteria.

  6. Integrated control system of transverse flow CO II laser and its application

    Science.gov (United States)

    Liu, Juan; Tang, Xiahui; Zhang, Yang; Peng, Hao; Wang, Youqing

    2008-03-01

    Aiming to the special high power CO II laser surface treatment, the paper developed the integrated control system based on S7-200 PLC of transverse flow CO II laser. The selection of key technology and components, detection and control of signals, integrated control of complete circuit, technology of human machine interface and process control of system have been researched. Double closed loop power control system was realized, so that the stability of the laser power was in +/-2%. Also, the giving power can be controlled by the laser controller or by the processing machine, thus, the users can control the laser more efficiently when processing. A series of experiments have been performed on 5kW transverse flow CO II laser, the output laser power was stable at discharge current of 9A for 8 hours, and the maximal power was 5.42 kW. The new type of transverse flow CO II Laser with Integrated Control System has been applied for special laser cladding with power-modulating on the metallic surface of the oil industry production.

  7. Implementation of a Proficiency-Based Diploma System in Maine: Phase II--District Level Analysis

    Science.gov (United States)

    Silvernail, David L.; Stump, Erika K.; McCafferty, Anita Stewart; Hawes, Kathryn M.

    2014-01-01

    This report describes the findings from Phase II of a study of Maine's implementation of a proficiency-based diploma system. At the request of the Joint Standing Committee on Education and Cultural Affairs of the Maine Legislature, the Maine Policy Research Institute (MEPRI) has conducted a two-phased study of the implementation of Maine law…

  8. Online Oxide Contamination Measurement and Purification Demonstration

    Science.gov (United States)

    Bradley, D. E.; Godfroy, T. J.; Webster, K. L.; Garber, A. E.; Polzin, K. A.; Childers, D. J.

    2011-01-01

    Liquid metal sodium-potassium (NaK) has advantageous thermodynamic properties indicating its use as a fission reactor coolant for a surface (lunar, martian) power system. A major area of concern for fission reactor cooling systems is system corrosion due to oxygen contaminants at the high operating temperatures experienced. A small-scale, approximately 4-L capacity, simulated fission reactor cooling system employing NaK as a coolant was fabricated and tested with the goal of demonstrating a noninvasive oxygen detection and purification system. In order to generate prototypical conditions in the simulated cooling system, several system components were designed, fabricated, and tested. These major components were a fully-sealed, magnetically-coupled mechanical NaK pump, a graphite element heated reservoir, a plugging indicator system, and a cold trap. All system components were successfully demonstrated at a maximum system flow rate of approximately 150 cc/s at temperatures up to 550 C. Coolant purification was accomplished using a cold trap before and after plugging operations which showed a relative reduction in oxygen content.

  9. Heteroatom incorporation effect in σ- and π-electron systems: the sEDA(II) and pEDA(II) descriptors.

    Science.gov (United States)

    Mazurek, Andrzej; Dobrowolski, Jan Cz

    2012-03-16

    The effect of heteroatom or heteroatomic group incorporation into unsaturated five- and six-membered cyclic systems was studied by means of DFT/B3LYP/aug-cc-pVDZ calculations. Two descriptors of the incorporation effect, sEDA(II) and pEDA(II), reflecting the influence of the incorporated atom or group on the population of the σ and π valence electrons, were constructed on the basis of natural bond orbital analysis. The sEDA(II) and pEDA(II) descriptors were shown to be linearly independent; the former correlated very well with electronegativity scales, whereas the latter correlated with NICS(1)(ZZ) and HOMA(CC) aromaticity indices. The two descriptors seem to be universal tools for analyzing different chemical and physicochemical effects occurring in unsaturated heterocyclic systems.

  10. Towards an Understanding of the Physical Nature of MgII Absorption Systems

    CERN Document Server

    Nestor, D B; Rao, S M; 10.1017/S1743921305002498

    2009-01-01

    We discuss issues concerning the physical nature of intervening MgII quasar absorption systems in light of results from our recent surveys using SDSS EDR QSO spectra and data obtained at the MMT. These surveys indicate an excess of weak $W_0^{\\lambda2796} \\lesssim 0.3$\\AA) systems relative to the exponential dN/dW distribution of stronger systems. The incidence of intermediate-strength lines shows remarkably little evolution with redshift, thereby constraining models for the nature of the clouds comprising these absorbers. The total distribution does evolve, with the incidence decreasing with decreasing redshift in a $W_0^{\\lambda2796}$-dependent rate (the strongest systems evolve the fastest). This suggests that multiple populations that evolve at different rates contribute to the incidence in a $W_0^{\\lambda2796}$-dependent manner. We also present two images of fields containing unprecedented ``ultra-strong'' ($W_0^{\\lambda2796} \\ge 4.0$\\AA) MgII absorbers.

  11. CPESIM II: A Computer System Simulation for Computer Performance Evaluation Use.

    Science.gov (United States)

    1983-12-01

    1 C C DATE: 5 DEC 1983 C C VERSION: 1.0 C C TITLE: CPESIM II Discrete File C FILENAME: SIMF C AUTHORt: David L. Owen C SOF’IWARE SYSTEM: CPESIM II C...itself. In the second phase the actual simulation programs, SIMS and SIMF , are used along with CONDES and EVSTR as input to generate performance data...shown in Figure 8. It is necessary to attach the network portion of the model (SIMS), the discrete portion of the model ( SIMF ), as well as the CONDES

  12. Evaluation of Quantum II microbiology system for identification of gram-negative bacteria of veterinary origin.

    OpenAIRE

    Jones, R. L.; Adney, W S; Davis, M A; Vonbyren, H; Thompson, G

    1987-01-01

    The ability of a rapid, semiautomated bacterial identification system, the Quantum II microbiology system (Abbott Laboratories, Irving, Tex.), to accurately identify gram-negative bacteria from veterinary sources was evaluated. A total of 378 isolates were tested, including 298 organisms in the family Enterobacteriaceae and strains representing Acinetobacter sp., Aeromonas sp., Flavobacterium sp., Pasteurella multocida, Plesiomonas sp., and Pseudomonas spp. Of these isolates, 333 (88.1%) were...

  13. Rotating Reverse-Osmosis for Water Purification

    Science.gov (United States)

    Lueptow, RIchard M.

    2004-01-01

    A new design for a water-filtering device combines rotating filtration with reverse osmosis to create a rotating reverse- osmosis system. Rotating filtration has been used for separating plasma from whole blood, while reverse osmosis has been used in purification of water and in some chemical processes. Reverse- osmosis membranes are vulnerable to concentration polarization a type of fouling in which the chemicals meant not to pass through the reverse-osmosis membranes accumulate very near the surfaces of the membranes. The combination of rotating filtration and reverse osmosis is intended to prevent concentration polarization and thereby increase the desired flux of filtered water while decreasing the likelihood of passage of undesired chemical species through the filter. Devices based on this concept could be useful in a variety of commercial applications, including purification and desalination of drinking water, purification of pharmaceutical process water, treatment of household and industrial wastewater, and treatment of industrial process water. A rotating filter consists of a cylindrical porous microfilter rotating within a stationary concentric cylindrical outer shell (see figure). The aqueous suspension enters one end of the annulus between the inner and outer cylinders. Filtrate passes through the rotating cylindrical microfilter and is removed via a hollow shaft. The concentrated suspension is removed at the end of the annulus opposite the end where the suspension entered.

  14. Blood purification and hemo- perfusion

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    The method of blood purification is a new overlapping frontierdiscipline which develops quickly in recent years. It helps overcoming many serious and complicated diseases, even including some incurable illnesses.

  15. Nanomechanical Water Purification Device Project

    Data.gov (United States)

    National Aeronautics and Space Administration — Seldon Laboratories, LLC, proposes a lightweight, low-pressure water purification device that harnesses the unique properties of carbon nanotubes and will operate...

  16. Janus II: the new generation Special Purpose Computer for spin-system simulations

    Science.gov (United States)

    Perez-Gaviro, Sergio; Janus Collaboration

    2014-03-01

    We present Janus II, our second grand challenge of High Performance Computing on Computational Physics. This Special Purpose Computer, recently developed and commissioned by the Janus Collaboration, is based on a Field-Programmable-Gate-Array (FPGA) architecture. Janus II has been designed and developed as a multipurpose reprogramable supercomputer and it is optimized for speeding up the Monte Carlo simulations of a wide class of spin glass models. It builds and improves on the experience of its predecessor,Janus, that has been successfully running physics simulations for the last 6 years. With Janus II will make possible to carry out Monte Carlo simulations campaigns that would take several centuries if performed on currently available computer systems.

  17. Multi-Objective PID-Controller Tuning for a Magnetic Levitation System using NSGA-II

    DEFF Research Database (Denmark)

    Pedersen, Gerulf K. M.; Yang, Zhenyu

    2006-01-01

    This paper investigates the issue of PID-controller parameter tuning for a magnetic levitation system using the non-dominated sorting genetic algorithm (NSGA-II). The magnetic levitation system is inherently unstable and the PID-controller parameters are hard to find using conventional methods....... Based on four different performance measures, derived from the step response of the levitation system, the algorithm is used to find a set of non-dominated parameters for a PID-controller that can stabilize the system and minimize the performance measures....

  18. Involvement of Spinal Angiotensin II System in Streptozotocin-Induced Diabetic Neuropathic Pain in Mice.

    Science.gov (United States)

    Ogata, Yoshiki; Nemoto, Wataru; Nakagawasai, Osamu; Yamagata, Ryota; Tadano, Takeshi; Tan-No, Koichi

    2016-09-01

    Renin-angiotensin system (RAS) activity increases under hyperglycemic states, and is thought to be involved in diabetic complications. We previously demonstrated that angiotensin (Ang) II, a main bioactive component of the RAS, might act as a neurotransmitter and/or neuromodulator in the transmission of nociceptive information in the spinal cord. Here, we examined whether the spinal Ang II system is responsible for diabetic neuropathic pain induced by streptozotocin (STZ). Tactile allodynia was observed concurrently with an increase in blood glucose levels the day after mice received STZ (200 mg/kg, i.v.) injections. Tactile allodynia on day 14 was dose-dependently inhibited by intrathecal administration of losartan, an Ang II type 1 (AT1) receptor antagonist, but not by PD123319, an AT2 receptor antagonist. In the lumbar dorsal spinal cord, the expression of Ang II, Ang converting enzyme (ACE), and phospho-p38 mitogen-activated protein kinase (MAPK) were all significantly increased on day 14 after STZ injection compared with vehicle-treated controls, whereas no differences were observed among AT1 receptors or angiotensinogen levels. Moreover, the increase in phospho-p38 MAPK was significantly inhibited by intrathecal administration of losartan. These results indicate that the expression of spinal ACE increased in STZ-induced diabetic mice, which in turn led to an increase in Ang II levels and tactile allodynia. This increase in spinal Ang II was accompanied by the phosphorylation of p38 MAPK, which was shown to be mediated by AT1 receptors.

  19. Angiotensin II in the paraventricular nucleus stimulates sympathetic outflow to the cardiovascular system and make vasopressin release in rat.

    Science.gov (United States)

    Khanmoradi, Mehrangiz; Nasimi, Ali

    2016-10-06

    The hypothalamic paraventricular nucleus (PVN) plays essential roles in neuroendocrine and autonomic functions, including cardiovascular regulation. It was shown that microinjection of angiotensin II (AngII) into the PVN produced a pressor response. In this study, we explored the probable mechanisms of this pressor response. AngII was microinjected into the PVN and cardiovascular responses were recorded. Then, the responses were re-tested after systemic injection of a ganglionic blocker, Hexamethonium, or a vasopressin V1 receptor blocker. Hexamethonium pretreatment (i.v.) greatly and significantly attenuated the pressor response to AngII, with no significant effect on heart rate, indicating that the sympathetic system is involved in the cardiovascular effect of AngII in the PVN. Systemic pretreatment (i.v.) with V1 antagonist greatly and significantly attenuated the pressor response to AngII, with no significant effect on heart rate, indicating that vasopressin release is involved in the cardiovascular effect of AngII in the PVN. Overall, we found that AngII microinjected into the PVN produced a pressor response mediated by the sympathetic system and vasopressin release, indicating that other than circulating AngII, endogenous AngII of the PVN increases the vasopressin release from the PVN. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

  20. 混合反胶团萃取α-淀粉酶%Purification of α-Amylase Using CTAB/Tween-60 Mixed Reverse Micellar System

    Institute of Scientific and Technical Information of China (English)

    高树刚; 宋伟明; 安红

    2011-01-01

    Reverse micellar system consisting of the surfactants cetyltrimethyl ammonium bromide(CTAB) and Tween-60 in dissolved in n-butanol-isooctane mixture was used for α-amylase purification by reversed micellar extraction.Extraction efficiency of α-amylase was investigated under different extraction conditions using a reverse micellar phase consisting of 4 g/L(CTAB +Tween-60,2.0:1.0,n/n) in n-butanol-isooctane mixture(1.0:1.0,V/V) and an aqueous phase made up of crude α-amylase solution containing 0.04 mol/L NaCl.The extraction efficiency of α-amylase was 91% when the extraction temperature,ratio of organic phase to aqueous phase and oscillation time were 40 ℃,2.0:1.0 and 10 min,respectively.The reverse extraction efficiency of α-amylase was 65% after 10 min oscillation at 50 ℃ when the aqueous phase was comprised of aqueous phase and organic phase at a ratio of 1.0:2.0(V/V) at pH 4.5 containing 2.5 mol/L NaCl.The reverse micelles could be repeatedly used and the extraction efficiency of α-amylase at an aqueous phase-to-organic phase ratio of 1.0:2.75 remained 71% in the second use.%以十六烷基三甲基溴化铵(CTAB)和脱水山梨醇单硬脂酸酯聚氧乙烯醚(Tween-60)为混合表面活性剂溶于正丁醇-异辛烷中构成反胶团系统,萃取纯化α-淀粉酶。研究不同萃取条件下,α-淀粉酶的萃取率。其中反胶团相组成为:ρ(CTAB+Tween-60)=4g/L;n(CTAB):n(Tween-60)=2.0:1.0;V(正丁醇):V(异辛烷)=1.0:1.0。水相组成为:α-淀粉酶配制的粗酶液,此时c(NaCl)=0.04mol/L,水相pH11.04。结果表明:萃取温度40℃、V(有机相):V(水相)=2.0:1.0、振荡时间10min时,α-淀粉酶萃取率可达91%;反萃取水相组成为c(NaCl)=2.5mol/L、pH4.5、V(水相):V(有机相)=1.0:2.0,反萃取振荡时间10min、温度50℃时,α-淀粉酶反萃取率可达65%。反胶团相可重复使用,当V

  1. Simulation model for wind energy storage systems. Volume II. Operation manual. [SIMWEST code

    Energy Technology Data Exchange (ETDEWEB)

    Warren, A.W.; Edsinger, R.W.; Burroughs, J.D.

    1977-08-01

    The effort developed a comprehensive computer program for the modeling of wind energy/storage systems utilizing any combination of five types of storage (pumped hydro, battery, thermal, flywheel and pneumatic). An acronym for the program is SIMWEST (Simulation Model for Wind Energy Storage). The level of detail of SIMWEST is consistent with a role of evaluating the economic feasibility as well as the general performance of wind energy systems. The software package consists of two basic programs and a library of system, environmental, and load components. Volume II, the SIMWEST operation manual, describes the usage of the SIMWEST program, the design of the library components, and a number of simple example simulations intended to familiarize the user with the program's operation. Volume II also contains a listing of each SIMWEST library subroutine.

  2. MONSTIR II: A 32-channel, multispectral, time-resolved optical tomography system for neonatal brain imaging

    Energy Technology Data Exchange (ETDEWEB)

    Cooper, Robert J., E-mail: robert.cooper@ucl.ac.uk; Magee, Elliott; Everdell, Nick; Magazov, Salavat; Varela, Marta; Airantzis, Dimitrios; Gibson, Adam P.; Hebden, Jeremy C. [Biomedical Optics Research Laboratory, Department of Medical Physics and Bioengineering, University College London, London WC1E 6BT (United Kingdom)

    2014-05-15

    We detail the design, construction and performance of the second generation UCL time-resolved optical tomography system, known as MONSTIR II. Intended primarily for the study of the newborn brain, the system employs 32 source fibres that sequentially transmit picosecond pulses of light at any four wavelengths between 650 and 900 nm. The 32 detector channels each contain an independent photo-multiplier tube and temporally correlated photon-counting electronics that allow the photon transit time between each source and each detector position to be measured with high temporal resolution. The system's response time, temporal stability, cross-talk, and spectral characteristics are reported. The efficacy of MONSTIR II is demonstrated by performing multi-spectral imaging of a simple phantom.

  3. MONSTIR II: A 32-channel, multispectral, time-resolved optical tomography system for neonatal brain imaging

    Science.gov (United States)

    Cooper, Robert J.; Magee, Elliott; Everdell, Nick; Magazov, Salavat; Varela, Marta; Airantzis, Dimitrios; Gibson, Adam P.; Hebden, Jeremy C.

    2014-05-01

    We detail the design, construction and performance of the second generation UCL time-resolved optical tomography system, known as MONSTIR II. Intended primarily for the study of the newborn brain, the system employs 32 source fibres that sequentially transmit picosecond pulses of light at any four wavelengths between 650 and 900 nm. The 32 detector channels each contain an independent photo-multiplier tube and temporally correlated photon-counting electronics that allow the photon transit time between each source and each detector position to be measured with high temporal resolution. The system's response time, temporal stability, cross-talk, and spectral characteristics are reported. The efficacy of MONSTIR II is demonstrated by performing multi-spectral imaging of a simple phantom.

  4. Interval Type-II Fuzzy Rule-Based STATCOM for Voltage Regulation in the Power System

    Directory of Open Access Journals (Sweden)

    Ying-Yi Hong

    2015-08-01

    Full Text Available The static synchronous compensator (STATCOM has recently received much attention owing to its ability to stabilize power systems and mitigate voltage variations. This paper investigates a novel interval type-II fuzzy rule-based PID (proportional-integral-derivative controller for the STATCOM to mitigate bus voltage variations caused by large changes in load and the intermittent generation of photovoltaic (PV arrays. The proposed interval type-II fuzzy rule base utilizes the output of the PID controller to tune the signal applied to the STATCOM. The rules involve upper and lower membership functions that ensure the stable responses of the controlled system. The proposed method is implemented using the NEPLAN software package and MATLAB/Simulink with co-simulation. A six-bus system is used to show the effectiveness of the proposed method. Comparative studies show that the proposed method is superior to traditional PID and type-I fuzzy rule-based methods.

  5. 25 CFR 547.10 - What are the minimum standards for Class II gaming system critical events?

    Science.gov (United States)

    2010-04-01

    ... 25 Indians 2 2010-04-01 2010-04-01 false What are the minimum standards for Class II gaming system critical events? 547.10 Section 547.10 Indians NATIONAL INDIAN GAMING COMMISSION, DEPARTMENT OF THE... GAMES § 547.10 What are the minimum standards for Class II gaming system critical events? This section...

  6. Influence of intellectual impairment (II on basketball players´ capacity to solve a game situation: towards evidence-based classification systems in II-basketball

    Directory of Open Access Journals (Sweden)

    Javier PINILLA

    2016-07-01

    Full Text Available Basketball for athletes with intellectual impairment (II requires the development of evidence-based eligibility systems to guarantee that only athletes with significant limitations performing basketball participate in these competitions. In addition, this is needed to re-include IIbasketball in the Paralympic program. To do this, it is required to investigate how II impacts on fundamental basketball activities. To address this need, the aim of this study was to compare ablebodied (AB and II-players´ capacity to solve a game situation. A field test was designed ad hoc in which 38 elite II-players from France, Portugal, Australia and Japan who participated in the Global Games (2015 and 38 AB-basketball players competing in Spanish competitions, had to solve 8 standardized game situations. Results indicated that II-players spent significantly (p≤0.05 more time to decide (0.92±0.2s than AB-players (0.63±0.1s and to execute the solution (II: 3.01±0.4s; AB: 2.56±0.3s. Also, II-players made more rule infractions (II: 7.11±1.4; AB: 7.71±0.7, more feints (II:1.37±1.4; AB: 0.3±0.6, more dribbles (II: 15.08±3.9; AB: 12.29±2.3 and they performed less successful solutions (II: 4.42±1.5; AB: 5.39±1.3. However, no differences were found in number of correct decisions made. Decision time, number of dribbles and situations in which they succeeded were the variables that best discriminated between II and AB-players. These results confirmed the negative influence of II on players´ capacity to solve a basketball game situation. The calculated discriminant function let us establish the minimum scores from this test that indicates significant limitations on the capacity to solve a game situation. These scores are applicable to determine eligibility criteria in IIbasketball worldwide.

  7. Emergency Response Equipment and Related Training: Airborne Radiological Computer System (Model II)

    Energy Technology Data Exchange (ETDEWEB)

    David P. Colton

    2007-02-28

    The materials included in the Airborne Radiological Computer System, Model-II (ARCS-II) were assembled with several considerations in mind. First, the system was designed to measure and record the airborne gamma radiation levels and the corresponding latitude and longitude coordinates, and to provide a first overview look of the extent and severity of an accident's impact. Second, the portable system had to be light enough and durable enough that it could be mounted in an aircraft, ground vehicle, or watercraft. Third, the system must control the collection and storage of the data, as well as provide a real-time display of the data collection results to the operator. The notebook computer and color graphics printer components of the system would only be used for analyzing and plotting the data. In essence, the provided equipment is composed of an acquisition system and an analysis system. The data can be transferred from the acquisition system to the analysis system at the end of the data collection or at some other agreeable time.

  8. Calibration of the Accuscan II In Vivo System for Whole Body Counting

    Energy Technology Data Exchange (ETDEWEB)

    Orval R. Perry; David L. Georgeson

    2011-08-01

    This report describes the April 2011 calibration of the Accuscan II HpGe In Vivo system for whole body counting. The source used for the calibration was a NIST traceable BOMAB manufactured by DOE as INL2006 BOMAB containing Eu-154, Eu-155, Eu-152, Sb-125 and Y-88 with energies from 27 keV to 1836 keV with a reference date of 11/29/2006. The actual usable energy range was 86.5 keV to 1597 keV on 4/21/2011. The BOMAB was constructed inside the Accuscan II counting 'tub' in the order of legs, thighs, abdomen, thorax/arms, neck, and head. Each piece was taped to the backwall of the counter. The arms were taped to the thorax. The phantom was constructed between the v-ridges on the backwall of the Accuscan II counter. The energy and efficiency calibrations were performed using the INL2006 BOMAB. The calibrations were performed with the detectors in the scanning mode. This report includes an overview introduction and records for the energy/FWHM and efficiency calibration including performance verification and validation counting. The Accuscan II system was successfully calibrated for whole body counting and verified in accordance with ANSI/HPS N13.30-1996 criteria.

  9. Calibration of the Accuscan II IN Vivo System for High Energy Lung Counting

    Energy Technology Data Exchange (ETDEWEB)

    Ovard R. Perry; David L. Georgeson

    2011-07-01

    This report describes the April 2011 calibration of the Accuscan II HpGe In Vivo system for high energy lung counting. The source used for the calibration was a NIST traceable lung set manufactured at the University of Cincinnati UCLL43AMEU & UCSL43AMEU containing Am-241 and Eu-152 with energies from 26 keV to 1408 keV. The lung set was used in conjunction with a Realistic Torso phantom. The phantom was placed on the RMC II counting table (with pins removed) between the v-ridges on the backwall of the Accuscan II counter. The top of the detector housing was positioned perpendicular to the junction of the phantom clavicle with the sternum. This position places the approximate center line of the detector housing with the center of the lungs. The energy and efficiency calibrations were performed using a Realistic Torso phantom (Appendix I) and the University of Cincinnati lung set. This report includes an overview introduction and records for the energy/FWHM and efficiency calibration including performance verification and validation counting. The Accuscan II system was successfully calibrated for high energy lung counting and verified in accordance with ANSI/HPS N13.30-1996 criteria.

  10. NSGA-II for optimizing water supply and hydroelectric power generation of Two-Reservoir System

    Science.gov (United States)

    Wang, K.; Chang, L.; Chang, F.

    2008-12-01

    Taiwan is located at the subtropical zone, which has nonuniform temporal and spatial distributions of precipitation. The Jhuoshuei River is the Taiwan longest river (186 Km) and has abundant water resources. In the last several decades, the constant population growth and the dramatic economic development have resulted in a tremendous demand for natural resources, especially water. How to effectively manage the water resources is one of the most important task for hydrologist. This paper presents a non-dominated sorting genetic algorithm (NSGA-II) to derive a set of optimal joint operating rule curves for a two-reservoir system, Wu-She Reservoir and Jih-Yueh-Tan Reservoir, in the Jhuoshuei river system. These two series reservoirs are the most important reservoirs for supplying water and hydroelectric power generation. The NSGA-II is coupled with an integrated operational simulation model to search the optimal joint rule curves for minimizing shortage indices (SI) and maximizing Hydroelectric Power generation. The Pareto-front solutions obtained from NSGA-II provide the decision maker with a wide range of choices. The results indicate that NSGA-II is a promising approach. The plentiful optimal solutions along the Pareto front allow the decision maker to choose the best compromise solution after consideration of the trade-offs between water supply and hydroelectric power generation.

  11. Fundamental limitations in the purifications of tensor networks

    Energy Technology Data Exchange (ETDEWEB)

    De las Cuevas, G.; Cirac, J. I. [Max Planck Institute for Quantum Optics, Hans-Kopfermann-Str. 1, 85748 Garching (Germany); Cubitt, T. S. [Department of Computer Science, University College London, London WC1E 6BT, United Kingdom and DAMTP, Centre for Mathematical Sciences, University of Cambridge, Wilberforce Road, Cambridge CB3 0WA (United Kingdom); Wolf, M. M. [Zentrum Mathematik, Technische Universität München, 85748 Garching (Germany); Pérez-García, D. [Departamento de Análisis Matemático and IMI, Universidad Complutense de Madrid, 28040 Madrid, Spain and ICMAT, C/ Nicolás Cabrera, Campus de Cantoblanco, 28049 Madrid (Spain)

    2016-07-15

    We show a fundamental limitation in the description of quantum many-body mixed states with tensor networks in purification form. Namely, we show that there exist mixed states which can be represented as a translationally invariant (TI) matrix product density operator valid for all system sizes, but for which there does not exist a TI purification valid for all system sizes. The proof is based on an undecidable problem and on the uniqueness of canonical forms of matrix product states. The result also holds for classical states.

  12. Fundamental limitations in the purifications of tensor networks

    Science.gov (United States)

    De las Cuevas, G.; Cubitt, T. S.; Cirac, J. I.; Wolf, M. M.; Pérez-García, D.

    2016-07-01

    We show a fundamental limitation in the description of quantum many-body mixed states with tensor networks in purification form. Namely, we show that there exist mixed states which can be represented as a translationally invariant (TI) matrix product density operator valid for all system sizes, but for which there does not exist a TI purification valid for all system sizes. The proof is based on an undecidable problem and on the uniqueness of canonical forms of matrix product states. The result also holds for classical states.

  13. Experimental implementation of edge nodes for an OPS system based on Nios II

    Science.gov (United States)

    Li, Xiaoyun; Xu, Bo; Qiu, Kun; Zhou, Heng

    2008-12-01

    The advantages of Optical Packet Switching (OPS) are transparent data transmission and data format, flexible packet control and dynamic resource allocation. It thus can support high-speed transmission of wideband data networks. Unlike Optical Burst Switching (OBS) systems which need reserved channel to transmit destination address, an advanced OPS solution using Optical Orthogonal Code (OOC) label for payload transfer has been proposed in this paper. Different from other OPS systems, our edge nodes are required to realize some special tasks including extracting destination addresses of the received IP packet which is used as payload in our OPS systems and sending the addresses to the OOC label generator. The OOC label will later be used to identify the destination of each payload at the core node for optical switching. Meanwhile, the higher-priority flow can be prior-serviced according to the Type of Service (TOS) in the head of IP packet. It is experimentally shown that the edge nodes in this OPS system can effectively improve the efficiency of packet switching and the speed of data transmission. The edge node implementation contains two parts: the hardware and software. For the hardware part, all necessary modules have been integrated onto one PCB board, thus avoiding possible instability caused by interconnecting different modules like transmitting/receiving Ethernet frame module, Field Programmable Gate Array (FPGA) processing module and optical transceiver module etc. from different Printed Circuit Boards (PCB). For the software part, the whole of system was based on MicroC/OS-II operating systems running on the Nios II soft core processor. It is verified by the experiment that embedded system designed with Nios II soft core CPU can help to speed up the hardware design. With the SOPC Builder's development environment, more attention can be paid to the structure and function of the system without worrying about the details of the circuit design, and better

  14. Intervening Mg II absorption systems from the SDSS DR12 quasar spectra

    CERN Document Server

    Raghunathan, Srinivasan; Campusano, Luis E; Söchting, Ilona K; Graham, Matthew J; Williger, Gerard M

    2016-01-01

    We present the catalogue of the Mg II absorption systems detected at a high significance level using an automated search algorithm in the spectra of quasars from the twelfth data release of the Sloan Digital Sky Survey. A total of 266,433 background quasars were searched for the presence of absorption systems in their spectra. The continuum modelling for the quasar spectra was performed using a mean filter. A pseudo-continuum derived using a median filter was used to trace the emission lines. The absorption system catalogue contains 39,694 Mg II systems detected at a 6.0, 3.0$\\sigma$ level respectively for the two lines of the doublet. The catalogue was constrained to an absorption line redshift of 0.35 $\\le$ z$_{2796}$ $\\le$ 2.3. The rest-frame equivalent width of the $\\lambda$2796 line ranges between 0.2 $\\le$ W$_r$ $\\le$ 6.2 \\AA. Using Gaussian-noise only simulations we estimate a false positive rate of 7.7 per cent in the catalogue. We measured the number density $\\partial N^{2796}/\\partial z$ of Mg II ab...

  15. Occurrence and activity of a type II CRISPR-Cas system in Lactobacillus gasseri.

    Science.gov (United States)

    Sanozky-Dawes, Rosemary; Selle, Kurt; O'Flaherty, Sarah; Klaenhammer, Todd; Barrangou, Rodolphe

    2015-09-01

    Bacteria encode clustered regularly interspaced short palindromic repeats (CRISPRs) and CRISPR-associated genes (cas), which collectively form an RNA-guided adaptive immune system against invasive genetic elements. In silico surveys have revealed that lactic acid bacteria harbour a prolific and diverse set of CRISPR-Cas systems. Thus, the natural evolutionary role of CRISPR-Cas systems may be investigated in these ecologically, industrially, scientifically and medically important microbes. In this study, 17 Lactobacillus gasseri strains were investigated and 6 harboured a type II-A CRISPR-Cas system, with considerable diversity in array size and spacer content. Several of the spacers showed similarity to phage and plasmid sequences, which are typical targets of CRISPR-Cas immune systems. Aligning the protospacers facilitated inference of the protospacer adjacent motif sequence, determined to be 5'-NTAA-3' flanking the 3' end of the protospacer. The system in L. gasseri JV-V03 and NCK 1342 interfered with transforming plasmids containing sequences matching the most recently acquired CRISPR spacers in each strain. We report the distribution and function of a native type II-A CRISPR-Cas system in the commensal species L. gasseri. Collectively, these results open avenues for applications for bacteriophage protection and genome modification in L. gasseri, and contribute to the fundamental understanding of CRISPR-Cas systems in bacteria.

  16. I. Textural/Structural tuning and nanoparticle stabilization of copper-containing nanocomposite materials. II. Generation of reducing agents for automotive exhaust gas purification via the processing of hydrocarbons in a PACT (plasma and catalysis integrated technologies) reactor

    Science.gov (United States)

    Xing, Yu

    This research consists of two parts. The first part deals with the preparation and properties of copper-containing nanocomposite materials. For studies of textural tuning, structural tuning, or material sintering, copper/aluminum and copper/zinc nanocomposites were prepared via various inorganic synthesis methods including conventional coprecipitation methods and a novel urea-gelation/thermal-modification method that produces narrow distributions of pore sizes, high surface areas, and significantly higher specific metal loadings. Solid-solid reaction analysis and differential scanning calorimetry (DSC) analysis were developed for the determination of the mixing homogeneities of the copper/aluminum nanocomposites. A sintering experiment at 250-600°C for 350 h under methanol-steam reforming conditions was carried out to compare the stability of supported Cu0 nanoparticles. The mixing homogeneities of CuO/Al2O3 nanocomposites significantly affected the thermal stability of their reduced Cu0 crystallites. Creation of relatively narrow distributions of pore sizes with relatively small major pore diameters (e.g., 3.5 nm) can also be used for the stabilization of supported Cu0 nanoparticles. The supported nanoparticles with a relatively small initial size cannot ensure good thermal stability. A "hereditary" character on the homogeneity of copper/aluminum nanocomposites was revealed. Stepwise reduction and reoxidation were studied for the structural tuning and purification of Cu-Al-O spinels with isotropic and gradual unit-cell contractions. The second part of the research deals with the processing of hydrocarbons. Conversion of a model hydrocarbon (n-hexane or n-octane) in an AC discharge PACT (plasma and catalysis integrated technologies) reactor was verified to be an effective method to instantly produce reducing agents (e.g., hydrogen or/and light alkanes and alkenes), at room temperature and atmospheric pressure for automotive exhaust gas purification. Effects of

  17. Analysis and treatment of trouble of molecular sieve purification system of 21000 m^3/h air separation plant%21000m^3/h空分设备分子筛纯化系统故障分析及处理

    Institute of Scientific and Technical Information of China (English)

    胥波; 闫伟东; 孔繁魁; 沈荣; 田现德

    2012-01-01

    In the air separation plant with molecular sieve adsorption and purification process, the work condition of molecular sieve purification system restricts the safe and stable run of the entire air separation system. For the troubles frequently occurring in molecular sieve purification system, such as mechanical trouble of switching over valve, valve action-generated program problem, and program-interlocked control of molecular sieve purification system, the judgment of the trouble causes and treatment measures are detailed.%分子筛吸附净化流程空分设备中,分子筛纯化系统的运行工况制约了整个空分系统的安全稳定运行。针对分子筛纯化系统经常出现的切换阀机械故障、阀门动作导致的程序问题以及分子筛纯化系统程序联锁控制等问题,详细介绍了故障原因判断和处理措施。

  18. Purification of equine chorionic gonadotropin (eCG) using magnetic ion exchange adsorbents in combination with high-gradient magnetic separation.

    Science.gov (United States)

    Müller, Christine; Heidenreich, Elena; Franzreb, Matthias; Frankenfeld, Katrin

    2015-01-01

    Current purification of the glycoprotein equine chorionic gonadotropin (eCG) from horse serum includes consecutive precipitation steps beginning with metaphosphoric acid pH fractionation, two ethanol precipitation steps, and dialysis followed by a numerous of fixed-bed chromatography steps up to the specific activity required. A promising procedure for a more economic purification procedure represents a simplified precipitation process requiring only onethird of the solvent, followed by the usage of magnetic ion exchange adsorbents employed together with a newly designed 'rotor-stator' type High Gradient Magnetic Fishing (HGMF) system for large-scale application, currently up to 100 g of magnetic adsorbents. Initially, the separation process design was optimized for binding and elution conditions for the target protein in mL scale. Subsequently, the magnetic filter for particle separation was characterized. Based on these results, a purification process for eCG was designed consisting of (i) pretreatment of the horse serum; (ii) binding of the target protein to magnetic ion exchange adsorbents in a batch reactor; (iii) recovery of loaded functionalized adsorbents from the pretreated solution using HGMF; (iv) washing of loaded adsorbents to remove unbound proteins; (v) elution of the target protein. Finally, the complete HGMF process was automated and conducted with either multiple single-cycles or multicycle operation of four sequential cycles, using batches of pretreated serum of up to 20 L. eCG purification with yields of approximately 53% from single HGMF cycles and up to 80% from multicycle experiments were reached, with purification and concentration factors of around 2,500 and 6.7, respectively.

  19. Design of the thermal neutron detection system for CJPL-II

    Science.gov (United States)

    Zeng, Zhao-Ming; Gong, Hui; Li, Jian-Min; Yue, Qian; Zeng, Zhi; Cheng, Jian-Ping

    2017-05-01

    A low background thermal neutron flux detection system has been designed to measure the ambient thermal neutron flux of the second phase of the China Jinping Underground Laboratory (CJPL-II), right after completion of the rock bolting work. A 3He proportional counter tube combined with an identical 4He proportional counter tube was employed as the thermal neutron detector, which has been optimised in energy resolution, wall effect and radioactivity of construction materials for low background performance. The readout electronics were specially designed for long-term stable operation and easy maintenance in an underground laboratory under construction. The system was installed in Lab Hall No. 3 of CJPL-II and accumulated data for about 80 days. The ambient thermal neutron flux was determined under the assumption that the neutron field is fully thermalized, uniform and isotropic at the measurement position. Supported by National Natural Science Foundation of China (11475094)

  20. Chemiluminescence of the peroxomonosulphate-cobalt(II)-aliphatic monocarboxylic acids system.

    Science.gov (United States)

    Wang, Min; Zhao, Lixia; Lin, Jin-Ming

    2007-01-01

    A weak chemiluminescence (CL) emission was observed due to the production of singlet oxygen ((1)O(2)) during the decomposition of peroxomonosulphate (HSO(5)(-)) catalysed by cobalt(II). Low molecular mass aliphatic monocarboxylic acids, such as formic, acetic, propionic, butyric and valeric acids, influenced the CL emission, and the reaction of aliphatic monocarboxylic acids with HSO(5)(-)/Co(2+) solution was further investigated using a flow injection analysis (FIA) CL method. The results indicated that the CL intensities of aliphatic monocarboxylic acids were improved with increase in the carbon chain length in the potassium peroxomonosulphate-cobalt(II) sulphate system. Generation of singlet oxygen was confirmed by the fact that the CL emission of aliphatic monocarboxylic acids with the HSO(5)(-)/Co(2+) solution was quenched by NaN(3), and from the CL spectrum of the reaction system. Additionally, a possible mechanism of aliphatic monocarboxylic acids CL emission enhancement was proposed.

  1. Software engineering for the EBR-II data acquisition system conversion

    Energy Technology Data Exchange (ETDEWEB)

    Schorzman, W.

    1988-01-01

    The original data acquisition system (DAS) for the Experimental Breeder Reactor II (EBR-II) was placed into service with state-of-the-art computer and peripherals in 1970. Software engineering principles for real-time data acquisition were in their infancy, and the original software design was dictated by limited hardware resources. The functional requirements evolved from creative ways to gather and display data. This abstract concept developed into an invaluable tool for system analysis, data reporting, and as a plant monitor for operations. In this paper the approach is outlined to the software conversion project with the restraints of operational transparency and 6 weeks for final conversion and testing. The outline is then compared with the formal principles of software engineering to show the way that bridge the gap can be bridged between the theoretical and real world by analyzing the work and listing the lessons learned.

  2. On the selection of damped Lyman α systems using Mg II absorption at 2 < zabs < 4

    Science.gov (United States)

    Berg, T. A. M.; Ellison, S. L.; Prochaska, J. X.; Sánchez-Ramírez, R.; Lopez, S.; D'Odorico, V.; Becker, G.; Christensen, L.; Cupani, G.; Denney, K.; Worseck, G.

    2017-01-01

    The XQ-100 survey provides optical and near-infrared coverage of 36 blindly selected, intervening damped Lyman α systems (DLAs) at 2 frame equivalent width (W0^{2796}) at these redshifts. Of the 29 DLAs with clean Mg II profiles, we find that six (20 per cent of DLAs) have W0^{2796} effect on the H I-weighted mean metallicity.

  3. Dimensional optimization of a minimally invasive surgical robot system based on NSGA-II algorithm

    OpenAIRE

    Wei Wang; Weidong Wang; Wei Dong; Hongjian Yu; Zhiyuan Yan; Zhijiang Du

    2015-01-01

    Based on the proposed end-effector structure of a laparoscopic minimally invasive surgical manipulator, a dimensional optimization method is investigated to enlarge the motion range of the mechanical arm in the specific target area and reduce the collision among the mechanical arms simultaneously. Both the length of the kinematics links and the overall size of the integrated system are considered in the optimization process. The NSGA-II algorithm oriented to the multi-objective optimization i...

  4. Oxford NOTECHS II: a modified theatre team non-technical skills scoring system.

    Directory of Open Access Journals (Sweden)

    Eleanor R Robertson

    Full Text Available BACKGROUND: We previously developed and validated the Oxford NOTECHS rating system for evaluating the non-technical skills of an entire operating theatre team. Experience with the scale identified the need for greater discrimination between levels of performance within the normal range. We report here the development of a modified scale (Oxford NOTECHS II to facilitate this. The new measure uses an eight-point instead of a four point scale to measure each dimension of non-technical skills, and begins with a default rating of 6 for each element. We evaluated this new scale in 297 operations at five NHS sites in four surgical specialities. Measures of theatre process reliability (glitch count and compliance with the WHO surgical safety checklist were scored contemporaneously, and relationships with NOTECHS II scores explored. RESULTS: Mean team Oxford NOTECHS II scores was 73.39 (range 37-92. The means for surgical, anaesthetic and nursing sub-teams were 24.61 (IQR 23, 27; 24.22 (IQR 23, 26 and 24.55 (IQR 23, 26. Oxford NOTECHS II showed good inter-rater reliability between human factors and clinical observers in each of the four domains. Teams with high WHO compliance had higher mean Oxford NOTECHS II scores (74.5 than those with low compliance (71.1 (p = 0.010. We observed only a weak correlation between Oxford NOTECHS II scores and glitch count; r = -0.26 (95% CI -0.36 to -0.15. Oxford NOTECHS II scores did not vary significantly between 5 different hospital sites, but a significant difference was seen between specialities (p = 0.001. CONCLUSIONS: Oxford NOTECHS II provides good discrimination between teams while retaining reliability and correlation with other measures of teamwork performance, and is not confounded by technical performance. It is therefore suitable for combined use with a technical performance scale to provide a global description of operating theatre team performance.

  5. Visual Data Analysis in the TJ-II Remote Participation System

    Energy Technology Data Exchange (ETDEWEB)

    Sanchez, E.; Portas, A.; Pereira, A.; Vega, J.

    2006-07-01

    A general-purpose data visualization tool has been developed to provide the TJ-II remote participation system with the same visualization capabilities already available in the TJ-II local environment. The visualization software has been developed in the Java language. It provides a user-friendly graphical interface that permits users on-demand plotting of time traces in a very flexible manner. In order to facilitate on-line tracking of experimental operation, the application also allows automatic refreshing of data. This software has been integrated into the TJ-II remote participation system distributed environment. Data are accessed remotely using web technologies and HTTP protocol and are transferred in a compressed format, which reduces bandwidth requirements. Both metadata and binary compressed data are transported in multipart messages. Message oriented middleware software is used to distribute information on-line, in particular notifications of data availability for automatic data refreshing or local events. Plot layouts can be stored in a centralized database for subsequent recovery from anywhere. Finally, this software is integrated into the general security framework provided by the PAPI system. (Author)

  6. Visual Data Analysis in the TJ-II Remote Participation System

    Energy Technology Data Exchange (ETDEWEB)

    Sanchez, E.; Porta, A.; Pereira, A.; Vega, J.

    2007-07-20

    A general-purpose data visualization tool has been developed to provide the TJ-II remote participation system with the same visualization capabilities already available in the TJ-II local environment. The visualization software has been developed in the Java language. It provides a user-friendly graphical interface that permits users on-demand plotting of time traces in a very flexible manner. In order to facilitate on-line tracking of experimental operation, the application also allows automatic refreshing of data. This software has been integrated into the TJ-II remote participation system distributed environment. Data are accessed remotely using web technologies and HTTP protocol and are transferred in a compressed format, which reduces bandwidth requirements. Both metadata and binary compressed data are transported in multi part messages. Message oriented middle ware software is used to distribute information on-line, in particular notifications of data availability for automatic data refreshing or local events. Plot layouts can be stored in a centralized database for subsequent recovery from anywhere. Finally, this software is integrated into the general security framework provided by the PAPI system. (Author) 16 refs.

  7. Evaluation of mono or mixed cultures of lactic acid bacteria in type II sourdough system.

    Science.gov (United States)

    Ekinci, Raci; Şimşek, Ömer; Küçükçuban, Ayca; Nas, Sebahattin

    2016-01-01

    The aim of this study was to evaluate the use of mono and mixed lactic acid bacteria (LAB) cultures to determine suitable LAB combinations for a type II sourdough system. In this context, previously isolated sourdough LAB strains with antimicrobial activity, which included Lactobacillus plantarum PFC22, Lactobacillus brevis PFC31, Pediococcus acidilactici PFC38, and Lactobacillus sanfranciscensis PFC80, were used as mono or mixed culture combinations in a fermentation system to produce type II sourdough, and subsequently in bread dough production. Compared to the monoculture fermentation of dough, the use of mixed cultures shortened the adaptation period by half. In addition, the use of mixed cultures ensured higher microbial viability, and enhanced the fruity flavor during bread dough production. It was determined that the combination of L. plantarum PFC22 + P. acidilactici PFC38 + L. sanfranciscensis PFC80 is a promising culture mixture that can be used in the production of type II sourdough systems, and that may also contribute to an increase in metabolic activity during bread production process.

  8. Protocol for Initial Purification of Bacteriocin

    Science.gov (United States)

    2015-10-01

    lysate/extract preparation , column purification, and a desalting. The peptide was tracked throughout the process using a soft agar overlay activity... PREPARATION SOLUBLE EXTRACTS COLUMN CHROMATOGRAPHY BACTERIA PURIFICATION CHARACTERIZATION...3  6.1  Preparation of Target Bacteria

  9. Adsorption of Pb(II on Mentha piperita carbon (MTC in single and quaternary systems

    Directory of Open Access Journals (Sweden)

    Rais Ahmad

    2017-02-01

    Full Text Available In the present study mentha treated carbon (MTC has been utilized as a novel adsorbent for the removal of Pb(II in single and quaternary systems from aqueous solution. The effects of various parameters like pH, contact time and ionic strength have been studied. The adsorbent was characterized by FTIR in order to find the functional groups present on the adsorbent. The equilibrium was attained in 180 min. The maximum adsorption of Pb(II was observed at pH 6. The adsorption isotherm studies show that data are fitted well with Freundlich and Temkin isotherms model. The kinetics data show that boundary layer diffusion is the rate controlling step for the adsorption process and it is dominant when Pb(II ion concentration is higher. The adsorption of Pb(II increases with the increase in the ionic strength of the solution. The positive value of ΔH0 indicates the reaction to be endothermic in nature. The activation energy was found to be 20.60 kJ/mol K indicating physiosorption.

  10. Purification of carbon nanotubes grown by thermal CVD

    Science.gov (United States)

    Porro, S.; Musso, S.; Vinante, M.; Vanzetti, L.; Anderle, M.; Trotta, F.; Tagliaferro, A.

    2007-03-01

    We show the results of a set of purifications on carbon nanotubes (CNT) by acid and basic treatments. CNTs were obtained by thermal decomposition of camphor at 850 °C in a CVD growth system, by means of a growth process catalyzed by iron clusters originating from the addition of ferrocene in the precursors mixture. The purification procedures involved HNO 3, H 2SO 4, HSO 3Cl and NaOH for different process temperatures. As-grown CNTs showed a consistent presence of metal catalyst (about 6 wt%), evidenced by TGA. The purification treatments led to a certain amount of opening of the CNT tips, with a consequent loss of metal catalyst encapsulated in tips. This is also confirmed by BET analysis, which showed an increase of the surface area density of CNT after the purification. FT-IR and XPS revealed the presence of carboxylic groups on the CNT surface chemically modified by the harsh environment of the purification process. Among the various treatments that have been tested, the 1:3 solution of nitric and sulphuric acid was the most effective in modifying the CNT surface and inducing the formation of functional groups.

  11. Rapid purification of recombinant histones.

    Directory of Open Access Journals (Sweden)

    Henrike Klinker

    Full Text Available The development of methods to assemble nucleosomes from recombinant histones decades ago has transformed chromatin research. Nevertheless, nucleosome reconstitution remains time consuming to this day, not least because the four individual histones must be purified first. Here, we present a streamlined purification protocol of recombinant histones from bacteria. We termed this method "rapid histone purification" (RHP as it circumvents isolation of inclusion bodies and thereby cuts out the most time-consuming step of traditional purification protocols. Instead of inclusion body isolation, whole cell extracts are prepared under strongly denaturing conditions that directly solubilize inclusion bodies. By ion exchange chromatography, the histones are purified from the extracts. The protocol has been successfully applied to all four canonical Drosophila and human histones. RHP histones and histones that were purified from isolated inclusion bodies had similar purities. The different purification strategies also did not impact the quality of octamers reconstituted from these histones. We expect that the RHP protocol can be readily applied to the purification of canonical histones from other species as well as the numerous histone variants.

  12. Incidence of HI 21-cm absorption in strong FeII systems at $0.5

    CERN Document Server

    Dutta, R; Gupta, N; Joshi, R; Petitjean, P; Noterdaeme, P; Ge, J; Krogager, J -K

    2016-01-01

    We present the results from our search for HI 21-cm absorption in a sample of 16 strong FeII systems ($W_{\\rm r}$(MgII $\\lambda2796$) $\\ge1.0$ \\AA\\ and $W_{\\rm r}$(FeII $\\lambda2600$) or $W_{\\rm FeII}$ $\\ge1$ \\AA) at $0.5II systems at this redshift range by $\\sim50$%. Combining our measurements with those in the literature, we find that the detection rate of HI 21-cm absorption increases with $W_{\\rm FeII}$, being four times higher in systems with $W_{\\rm FeII}$ $\\ge1$ \\AA\\ compared to systems with $W_{\\rm FeII}$ $<1$ \\AA. The $N$(HI) associated with the HI 21-cm absorbers would be $\\ge 2 \\times 10^{20}$ cm$^{-2}$, assuming a spin temperature of $\\sim500$ K (based on HI 21-cm absorption measurements of damped Lyman-$\\alpha$ systems at this redshift range) and unit covering factor. We find that ...

  13. Pivotal results for the Medtronic Valiant Thoracic Stent Graft System in the VALOR II trial.

    Science.gov (United States)

    Fairman, Ronald M; Tuchek, J Michael; Lee, W Anthony; Kasirajan, Karthikeshwar; White, Rodney; Mehta, Manish; Lyden, Sean; Mukherjee, Dipankar; Bavaria, Joseph

    2012-11-01

    We report 30-day and 12-month results of endovascular treatment with the Valiant Thoracic Stent Graft System (Medtronic Vascular, Santa Rosa, Calif) in patients with descending thoracic aortic aneurysms of degenerative etiology. The Valiant stent graft is an evolution of the Talent thoracic stent graft (Medtronic Vascular). The VALOR II (Evaluation of the Clinical Performance of the Valiant Thoracic Stent Graft System in the Treatment of Descending Thoracic Aneurysms of Degenerative Etiology in Subjects Who Are Candidates for Endovascular Repair) was a prospective, nonrandomized, pivotal trial conducted at 24 U.S. sites with enrollment between December 2006 and September 2009. Standard follow-up examinations, including physical examination, computed tomography, and chest radiography, were at 1, 6, and 12 months, and annually through 5 years. VALOR II outcomes were compared with those from the pivotal VALOR (Evaluation of the Medtronic Vascular Talent Thoracic Stent Graft System for the Treatment of Thoracic Aortic Aneurysms) trial of the Talent stent graft, which enrolled 195 patients with similar enrollment criteria. VALOR II enrolled 160 patients. Compared with VALOR patients, VALOR II patients had similar age and sex distribution but higher rates of cardiovascular risk factors and significantly more severe modified Society for Vascular Surgery/American Association for Vascular Surgery risk scores. Stent graft delivery and deployment were successful in 154 patients (96.3%). Outcomes at 30 days in VALOR II were perioperative mortality, 3.1%; major adverse events, 38.1%; paraplegia, 0.6%; paraparesis, 1.9%; and stroke, 2.5%. At 12 months, after the minimum sample size was reached, 151 patients were evaluated: aneurysm-related mortality was 4.0%, stent graft migration was 2.9%, and endoleak was 13.0%. Through 12 months, there were no ruptures, conversions to open surgery, secondary procedures due to endoleak >30 days, or loss of stent graft patency. The Valiant

  14. Results of FY 2001 feasibility studies on commercialized fast reactor cycle system phase-II

    Energy Technology Data Exchange (ETDEWEB)

    Noda, Hiroshi; Yamashita, Hidetoshi; Maeda, Fumio; Sato, Kazujiro; Ieda, Yoshiaki; Funasaka, Hideyuki [Japan Nuclear Cycle Development Inst., Tokai, Ibaraki (Japan)

    2002-09-01

    Feasibility Studies on Commercialized Fast Reactor (FR) Cycle System Phase-II were commenced on April 1, 2001, in order to select a few promising candidate concepts for commercialization from the candidate concepts of the FR system and fuel cycle system which were screened in Phase-I, and to present an outline plan for Phase-III onward. In FY 2001, which was the first year of Phase-II, the results of Phase-I and the plan for Phase-II were evaluated as appropriate by The R and D Project Evaluation Committee. With regard to the sodium-cooled medium-scale modular reactor and lead-bismuth cooled modular reactor, economical targets are expected to be achieved. In terms of the gas-cooled reactor, the helium gas-cooled reactor (coated particle fuel type and dispersion fuel type) was screened as a candidate concept. For the reprocessing system, a feasibility of the process for the crystallization method on the advanced aqueous method was confirmed. With regard to the oxide electrowinning method, the technological feasibility of MOX electrowinning co-precipitation was confirmed. In terms of the metal electrowinning method, the possibility of system rationalization was confirmed by Pu recovery testing at liquid Cd cathode. For the fuel fabrication system, in terms of the pelletizing method, the ease of remote-controlled fabrication of low-decontamination TRU fuels was confirmed, and in terms of the vibration compaction method, the packing density is expected to be satisfied as regards the design requirement. With regard to the casting method, the operation parameters of the injection casting technology, which were satisfied to slug specification requirements, were grasped by engineering-scale testing. (author)

  15. Purification of Nanoparticles by Size and Shape

    Science.gov (United States)

    Robertson, James D.; Rizzello, Loris; Avila-Olias, Milagros; Gaitzsch, Jens; Contini, Claudia; Magoń, Monika S.; Renshaw, Stephen A.; Battaglia, Giuseppe

    2016-06-01

    Producing monodisperse nanoparticles is essential to ensure consistency in biological experiments and to enable a smooth translation into the clinic. Purification of samples into discrete sizes and shapes may not only improve sample quality, but also provide us with the tools to understand which physical properties of nanoparticles are beneficial for a drug delivery vector. In this study, using polymersomes as a model system, we explore four techniques for purifying pre-formed nanoparticles into discrete fractions based on their size, shape or density. We show that these techniques can successfully separate polymersomes into monodisperse fractions.

  16. SO/sub 2/ and NO/sub x/ gas purification system producing sodium sulfate as final product. SO/sub 2/- und NO/sub x/-Gasreinigungsanlage mit Endprodukt Natriumsulfat

    Energy Technology Data Exchange (ETDEWEB)

    Hoelter, H.; Igelbuescher, H.; Gresch, H.; Dewert, H.

    1987-04-30

    The flue gas purification method is, according to invention, based on the following process: In the first scrubber the SO/sub 2/ is separated with the help of a sodium sulfite solution, namely by formation of sodium bisulfite (NaHSO/sub 3/). Then the NO/sub x/-containing flue gas is washed in the second scrubber with a EDTA solution. In a crystallizer placed behind the first scrubber pure SO/sub 2/ gas is regenerated from the NaHSO/sub 3/ solution and part of the SO/sub 2/ gas is used for the reduction of Fe-III into Fe-II and of NO/sub x/ into N/sub 2/ and sulfate. Furthermore, Na/sub 2/SO/sub 4/ is released from the crystallizer. Part of the scrubbing solution from the second scrubber is left to thermal decomposition at about 650/sup 0/C for the destruction of the N/S salts with the released SO/sub 2/ being recirculated into the first scrubber and the Na/sub 2/SO/sub 4/ residue being discharged.

  17. Multi-tier approach for data acquisition programming in the TJ-II remote participation system

    Science.gov (United States)

    Vega, J.; Sánchez, E.; Portas, A.; Ruiz, M.; Barrera, E.; López, S.

    2004-10-01

    Programming software to setup acquisition channels during device operation has been developed for the TJ-II remote participation system. The software follows a three-tier model. A first tier (client tier) groups client software containing only user interface code. A second tier (middle tier) includes code for authorization, authentication, and query processing. A third tier (data tier) consists of a relational database server for managing configurations. Multi-platform characteristics are provided by web browsers (client tier) and web servers (middle tier). This architecture avoids that data acquisition system controllers provide access control, database support, or graphic user interface resources. Therefore, computation capabilities of these systems can mainly be devoted to data handling. LabView (from National Instruments) has been used as programming language in the acquisition systems. This design allows a very transparent management of signals, independently on hardware modules and systems.

  18. Design of Networked Home Automation System Based on μCOS-II and AMAZON

    Directory of Open Access Journals (Sweden)

    Liu Jianfeng

    2015-01-01

    Full Text Available In recent years, with the popularity of computers and smart phones and the development of intelligent building in electronics industry, people’s requirement of living environment is gradually changing. The intelligent home furnishing building has become the new focus of people purchasing. And the networked home automation system which relies on the advanced network technology to connect with air conditioning, lighting, security, curtains, TV, water heater and other home furnishing systems into a local area network becomes a networked control system. μC /OS is a real-time operating system with the free open-source code, the compact structure and the preemptive real-time kernel. In this paper, the author focuses on the design of home furnishing total controller based on AMAZON multimedia processor and μC/OS-II real-time operating system, and achieves the remote access connection and control through the Ethernet.

  19. Dynamic Behaviors of Holling Type II Predator-Prey System with Mutual Interference and Impulses

    Directory of Open Access Journals (Sweden)

    Hongli Li

    2014-01-01

    Full Text Available A class of Holling type II predator-prey systems with mutual interference and impulses is presented. Sufficient conditions for the permanence, extinction, and global attractivity of system are obtained. The existence and uniqueness of positive periodic solution are also established. Numerical simulations are carried out to illustrate the theoretical results. Meanwhile, they indicate that dynamics of species are very sensitive with the period matching between species’ intrinsic disciplinarians and the perturbations from the variable environment. If the periods between individual growth and impulse perturbations match well, then the dynamics of species periodically change. If they mismatch each other, the dynamics differ from period to period until there is chaos.

  20. Status of LCLS - II QA Systems Collaboration for Cyromodule Construction at TJNAF and FNAL

    Energy Technology Data Exchange (ETDEWEB)

    McEwen, E. A. [Jefferson Lab; Leung, J. [Jefferson Lab; Bookwalter, V. [Jefferson Lab; Blowers, J. [Fermilab; Szal, J. [Fermialb

    2015-09-25

    At the Thomas Jefferson National Accelerator Facility (Jefferson Lab), we are supporting the LCLS-II Project at SLAC. The plan is to build thirty-five 1.3 GHz continuous wave cryomodules, production to be split between JLab and FNAL (Fermilab). This has required a close collaboration between the partner labs, including enhancing our existing quality systems to include this collaboration. This overview describes the current status of the Quality System development as of August 2015, when the partner labs start the assembly of the prototype cryomodules.

  1. Tank waste information network system II (TWINS2) year 2000 compliance assurance plan

    Energy Technology Data Exchange (ETDEWEB)

    Adams, M.R.

    1998-04-16

    The scope of this plan includes the Tank Waste Information Network System II (TWINS2) that contains the following major components: Tank Characterization Database (TCD), Tank Vapor Database (TVD), Data Source Access (DSA), automated Tank Characterization Report, Best-Basis Inventory Model (BBIM), and Tracker (corrective action tracking) function. The automated Tank Characterization Report application currently in development also will reside on-the TWINS system as will the BBIM. Critical inputs to TWINS occur from the following databases: Labcore and SACS. Output does not occur from TWINS to these two databases.

  2. Electronic readout system for the Belle II imaging Time-Of-Propagation detector

    Science.gov (United States)

    Kotchetkov, Dmitri

    2017-07-01

    The imaging Time-Of-Propagation (iTOP) detector, constructed for the Belle II experiment at the SuperKEKB e+e- collider, is an 8192-channel high precision Cherenkov particle identification detector with timing resolution below 50 ps. To acquire data from the iTOP, a novel front-end electronic readout system was designed, built, and integrated. Switched-capacitor array application-specific integrated circuits are used to sample analog signals. Triggering, digitization, readout, and data transfer are controlled by Xilinx Zynq-7000 system on a chip devices.

  3. Investigation of Propellant and Explosive Solid Solution Systems II X-Ray Studies

    Science.gov (United States)

    1978-03-01

    A\\Yj* ^\\C/*^ ^ 1 tatf AD 7t ott w AD-E400 125 TECHNICAL REPORT ARLCD-TR-77066 INVESTIGATION OF PROPELLANT AND EXPLOSIVE SOLID SOLUTION SYSTEMS...Report ARLCD-TR-77066 2. GOVT ACCESSION NO. *. TITLE (and Subtitle) INVESTIGATION OF PROPELLANT AND EXPLOSIVE SOLID SOLUTION SYSTEMS II X-RAY...Interplanar spacings and x-ray diffraction 9 intensities of AP, KP and their physical mixtures and solid solutions 4 X-ray data of 3 AN: KP solid solution and

  4. The Status of KamLAND After Purification

    Science.gov (United States)

    Grant, Christopher

    2010-02-01

    KamLAND is a 1-kton liquid scintillation detector located in the Kamioka underground laboratory, in Japan. KamLAND has provided a precision measurement of δm^221 using reactor anti-neutrinos, and yielded first observational evidence of geologically produced anti-neutrinos. Since April of 2007, the collaboration has been working on the purification of the detector with the goal of observing 862 keV, ^7Be solar neutrinos. Two purification campaigns have concluded, with a total of 5.4 ktons of scintillator circulated through a distillation and nitrogen purge system. The results of purification and the overall background reduction factors will be presented, along with an update of the ^7Be solar neutrino analysis. )

  5. [Reduction of nitrobenzene by iron oxides bound Fe(II) system at different pH values].

    Science.gov (United States)

    Luan, Fu-Bo; Xie, Li; Li, Jun; Zhou, Qi

    2009-07-15

    Batch tests were conducted to investigate the reductive transformation of nitrobenzene by goethite, hematite, magnetite and steel converter slag bound Fe(II) system. And the reduction mechanism was explored at different pH values. Experimental results showed that hematite, magnetite and steel converter slag could adsorb Fe(II) on surfaces and form iron oxides bound Fe(II) system at pH from 6.5 to 7.0. The systems had strong reductive capacity and could reduce nitrobenzene to aniline. The reduction efficiency of nitrobenzene in surface bound Fe(II) system followed the sequence of magnetite, hematite and steel converter slag from high to low. The reduction efficiency of hematite and magnetite system increased with pH increasing. While it was almost pH independent in steel converter slag system. Although goethite adsorbed most of Fe(II) in solution, the adsorbed Fe(II) had no reductive activity for nitrobenzene. At pH 6.0, small amount of Fe(II) was adsorbed on magnetite and hematite and the systems did not show reductive activity for nitrobenzene. However, steel converter slag could adsorb Fe(II) at pH 6.0 and reduction efficiency almost equaled to the value at pH 7.0. When pH was above 7.5, dissolved Fe(II) could be converted to Fe(OH)2 and the newly formed Fe(OH)2 became the main redactor in the system. Under alkali condition, the presence of iron oxides inhibited the reduction capacity of system.

  6. RELIGION AND PURIFICATION OF SOUL

    Directory of Open Access Journals (Sweden)

    Azam Khodashenas Pelko

    2010-11-01

    Full Text Available The Jainism emphasizes three major teachings about the purification of the soul (jiva, Ahimsa, Aparigrapha and anekantwad. Jainism, The focus of this religion has been purification of the soul by means of right conduct, right faith and right knowledge. The ultimate goal of Hinduism is Moksha or liberation (total freedom. In Hinduism, purification of the soul is a goal that one must work to attain. The Buddhism is the science of pursuing the aim of making the human mind perfect, and of purifying the human soul. The knowledge of purifying of the soul and softening of the hearts is as essential for human. They having the correct motivations means purifying our souls from hypocrisy, caprice, and heedlessness. The primary goal of Taoism may be described as the mystical intuition of the Tao, which is the way, the undivided unity, and the ultimate Reality. According to the Christianity access to truth cannot be conceived without purity of the soul

  7. Calibration of the Accuscan II In Vivo System for I-131 Thyroid Counting

    Energy Technology Data Exchange (ETDEWEB)

    Orval R. Perry; David L. Georgeson

    2011-07-01

    This report describes the March 2011 calibration of the Accuscan II HpGe In Vivo system for I-131 thyroid counting. The source used for the calibration was an Analytics mixed gamma source 82834-121 distributed in an epoxy matrix in a Wheaton Liquid Scintillation Vial with energies from 88.0 keV to 1836.1 keV. The center of the detectors was position 64-feet from the vault floor. This position places the approximate center line of the detectors at the center line of the source in the thyroid tube. The calibration was performed using an RMC II phantom (Appendix J). Validation testing was performed using a Ba-133 source and an ANSI N44.3 Phantom (Appendix I). This report includes an overview introduction and records for the energy/FWHM and efficiency calibrations including verification counting. The Accuscan II system was successfully calibrated for counting the thyroid for I-131 and verified in accordance with ANSI/HPS N13.30-1996 criteria.

  8. Event-driven timing system based on MRF cPCI hardware for HLS-II

    Institute of Scientific and Technical Information of China (English)

    李川; 王季刚; 宣科; 刘功发; 王琳; 李为民; 李京袆

    2015-01-01

    Hefei Light Source (HLS)-II is a vacuum ultraviole (VUV) synchrotron light source. A major upgrade of the light source was finished at the end of 2014. The timing system was rebuilt using compact peripheral component interconnect (cPCI) event-driven hardware to meet synchronization requirements of the machine. In the new system, the cPCI event-driven products manufactured by the micro-research finland (MRF) Oy are employed to achieve about 100 output signals with different interfaces. Device supports and drivers developed for common Experimental Physics and Industrial Control System (EPICS) records are used to access the registers on the timing modules. Five cPCI-bus input/output controllers (IOCs) distributed in different areas of the light source host timing modules for various subsystems. The delay resolution of this system is 9.8 ns for most channels and 9 ps for the channels used for triggering the electron gun and the injection kickers. The measured rms jitter of the output signal is less than 27 ps. Using the bucket chooser, this system enables the HLS-II to fill the storage ring with any designated bunch pattern. Benefitting from this upgrade, brightness and performance of the light source are significantly improved.

  9. Design and Construction of the TOPAZ II Reactor System Real-Time Dynamic Simulator

    Science.gov (United States)

    Kwok, Kwan S.

    1994-07-01

    A dynamic simulator of the TOPAZ II reactor system has been developed for the Nuclear Electric Propulsion Space Test Program. The simulator is a self-contained IBM-PC compatible based system that executes at a speed faster than real-time. The CPU is an 80486 DX2 processor operating at 66 MHz. The data acquisition system also employs an 80486 processor at 24 MHz on board. The data acquisition system is capable of providing 128 channels of analog-to-digital inputs at 1.3 MHz simultaneously, 64 channels of digital inputs at 1.6 MHz on a single channel, 64 channels of digital outputs at 1.6 MHz on a single channel, and 66 digital-to-analog channels at 1.6 MHz on a single channel. The simulator software operates in the Windows environment. The overall execution time of the simulator for each time step is 15 ms when no data is written to the disk, and 18 ms when nine double precision data points are written to the disk once in every time step. The simulator combines first-principle modeling and empirical correlations in its algorithm to attain the modeling accuracy and computational through-put that are required for real-time execution. It has been found to provide realistic real-time dynamic response of the TOPAZ II reactor system.

  10. PROTEGE-II: computer support for development of intelligent systems from libraries of components.

    Science.gov (United States)

    Musen, M A; Gennari, J H; Eriksson, H; Tu, S W; Puerta, A R

    1995-01-01

    PROTEGE-II is a suite of tools that facilitates the development of intelligent systems. A tool called MAiTRE allows system builders to create and refine abstract models (ontologies) of application domains. A tool called DASH takes as input a modified domain ontology and generates automatically a knowledge-acquisition tool that application specialists can use to enter the detailed content knowledge required to define particular applications. The domain-dependent knowledge entered into the knowledge-acquisition tool is used by assemblies of domain-independent problem-solving methods that provide the computational strategies required to solve particular application tasks. The result is an architecture that offers a divide-and-conquer approach that separates system-building tasks that require skill in domain analysis and modeling from those that require simple entry of content knowledge. At the same time, applications can be constructed from libraries of component--of both domain ontologies and domain-independent problem-solving methods--allowing the reuse of knowledge and facilitating ongoing system maintenance. We have used PROTEGE-II to construct a number of knowledge-based systems, including the reasoning components of T-Helper, which assists physicians in the protocol-based care of patients who have HIV infection.

  11. A Fire Safety Certification System for Board and Care Operators and Staff. SBIR Phase II: Final Report.

    Science.gov (United States)

    Walker, Bonnie L.

    This report describes Phase II of a project which developed a system for delivering fire safety training to board and care providers who serve adults with developmental disabilities. Phase II focused on developing and pilot testing a "train the trainers" workshop for instructors and field testing the provider's workshop. Evaluation of the 2-day…

  12. 25 CFR 547.7 - What are the minimum technical hardware standards applicable to Class II gaming systems?

    Science.gov (United States)

    2010-04-01

    ... 25 Indians 2 2010-04-01 2010-04-01 false What are the minimum technical hardware standards applicable to Class II gaming systems? 547.7 Section 547.7 Indians NATIONAL INDIAN GAMING COMMISSION... OF CLASS II GAMES § 547.7 What are the minimum technical hardware standards applicable to Class...

  13. 25 CFR 547.6 - What are the minimum technical standards for enrolling and enabling Class II gaming system...

    Science.gov (United States)

    2010-04-01

    ... 25 Indians 2 2010-04-01 2010-04-01 false What are the minimum technical standards for enrolling and enabling Class II gaming system components? 547.6 Section 547.6 Indians NATIONAL INDIAN GAMING... WITH THE PLAY OF CLASS II GAMES § 547.6 What are the minimum technical standards for enrolling and...

  14. Multigroup Confirmatory Factor Analysis for the Teacher Form, Ages 5 to 21, of the Adaptive Behavior Assessment System-II

    Science.gov (United States)

    Aricak, O. Tolga; Oakland, Thomas

    2010-01-01

    The American Association on Intellectual and Developmental Disabilities has promulgated various models of adaptive behavior, including its 1992 model that highlighted 10 adaptive skills and its 2002 model that highlighted three conceptual domains. The Adaptive Behavior Assessment System-II (ABAS-II) was designed to be consistent with these models.…

  15. TEMPERATURE DEPENDENCE OF 57Fe-MÖSSBAUER SPECTRA FOR A 〖〖Fe〗_Fc^II-Fe〗_tpy^II-〖Fe〗_Fc^II TRINUCLEAR SYSTEM

    Directory of Open Access Journals (Sweden)

    Dumitru Sirbu

    2015-06-01

    Full Text Available 57Fe Mӧssbauer spectra were recorded for 1'-terpyridine ferrocenecarboxylic acid and [bis(1'-terpyridine ferrocenecarboxylic acid Fe(II]2+ in the temperature range 7 – 293 K. The temperature dependence of the Quadrupole Splitting, Isomer Shift and Debye-Waller factor are discussed. The Debye temperature for the iron nuclei in the investigated compounds was determined.

  16. Intervening Mg II absorption systems from the SDSS DR12 quasar spectra

    Science.gov (United States)

    Raghunathan, Srinivasan; Clowes, Roger G.; Campusano, Luis E.; Söchting, Ilona K.; Graham, Matthew J.; Williger, Gerard M.

    2016-12-01

    We present the catalogue of the Mg II absorption systems detected at a high significance level using an automated search algorithm in the spectra of quasars from the 12th data release of the Sloan Digital Sky Survey. A total of 266,433 background quasars were searched for the presence of absorption systems in their spectra. The continuum modelling for the quasar spectra was performed using a mean filter. A pseudo-continuum derived using a median filter was used to trace the emission lines. The absorption system catalogue contains 39,694 Mg II systems detected at a 6.0, 3.0σ level respectively for the two lines of the doublet. The catalogue was constrained to an absorption line redshift of 0.35 ≤ z2796 ≤ 2.3. The rest-frame equivalent width of the λ2796 line ranges between 0.2 ≤ Wr ≤ 6.2 Å. Using Gaussian noise-only simulations, we estimate a false positive rate of 7.7 per cent in the catalogue. We measured the number density ∂N2796/∂z of Mg II absorbers and find evidence for steeper evolution of the systems with Wr ≥ 1.2 Å at low redshifts (z2796 ≤ 1.0), consistent with other earlier studies. A suite of null tests over the redshift range 0.5 ≤ z2796 ≤ 1.5 was used to study the presence of systematics and selection effects like the dependence of the number density evolution of the absorption systems on the properties of the background quasar spectra. The null tests do not indicate the presence of any selection effects in the absorption catalogue if the quasars with spectral signal-to-noise level less than 5.0 are removed. The resultant catalogue contains 36,981 absorption systems. The Mg II absorption catalogue is publicly available and can be downloaded from the link http://srini.ph.unimelb.edu.au/mgii.php.

  17. PREPARATIVE ISOLATION AND PURIFICATION OF CHEMICAL CONSTITUENTS OF BELAMCANDA BY MPLC, HSCCC AND PREP-HPLC

    Science.gov (United States)

    Wang, Xiaohong; Liang, Yong; Peng, Cuilin; Xie, Huichun; Pan, Man; Zhang, Tianyou; Ito, Yoichiro

    2010-01-01

    Combined with medium-pressure liquid chromatography (MPLC) and preparative high-pressure liquid chromatography (Prep-HPLC), high-speed countercurrent chromatography (HSCCC) was successfully applied for separation and purification of isoflavonoids from the extract of belamcanda. HSCCC separation was performed on a two-phase solvent system composed of methyl tert-butyl ether -ethyl acetate - n-butyl alcohol – acetonitrile −0.1% aqueous trifluoroacetic acid at a volume radio of 1:2:1:1:5. Semi-purified peak fractions from HSCCC separation were further purified by Prep-HPLC. Nine well-separated fractions were analyzed by HPLC-UV absorption spectrometry to determine their purities and characterized with ESI-MSn. Except for peaksland VII (unknown) seven compounds were identified as apocynin (peak II), mangiferin (peak III), 7-O-methylmangiferin (peak IV), hispidulin (peak V), 3′-hydroxyltectoridin (peak VI), iristectorin B (peak VII), isoiridin (peak IX). PMID:21552369

  18. A spectroscopic investigation of captopril and the Cu(II) captopril system

    Science.gov (United States)

    Torreggiani, Armida; Taddei, Paola; Tosi, M. Raffaella; Tugnoli, Vitaliano

    2001-05-01

    A Raman and IR study of captopril (CpSH), a synthetic derivative of L-proline, and the Cu(II)-CpSH system at different pHs and metal/ligand ratios was carried out. The vibrational spectra suggested disulphide formation (CpSSCp) by the reduction of Cu(II) to Cu(I) and allowed the identification of the sites involved in metal coordination. Various complexes can be formed and the nature of the predominant species depends mainly on the pH. At pH 10 CpSH gives rise to two monomeric complexes with different structures depending on the metal/ligand ratio, whereas at acid pH a water-insoluble polymeric species predominates.

  19. Hydrogen purification by periodic adsorption

    Energy Technology Data Exchange (ETDEWEB)

    Barg, Christian; Secchi, Argimiro R.; Trierweiler, Jorge O. [Rio Grande do Sul Univ., Porto Alegre, RS (Brazil). Dept. de Engenharia Quimica]. E-mail: cbarg@enq.ufrgs.br; arge@enq.ufrgs.br; jorge@enq.ufrgs.br

    2000-07-01

    The periodic adsorption processes have been widely used for industrial applications, mainly because it spends less energy than the usual gas separation processes, like the cryogenic distillation. The largest commercial application of periodic adsorption processes is the pressure swing adsorption (PSA) applied to hydrogen purification. Although its wide use in the chemical and petrochemical industry, there are no reports in the open literature about complete modeling studies of a complex commercial unit, with multiple adsorbents and multiple beds and several feed components. This study has as objective the modeling, optimization and dynamical analysis of an industrial PSA unit for hydrogen purification. (author)

  20. Keeping the wolves at bay: antitoxins of prokaryotic type II toxin-antitoxin systems

    Directory of Open Access Journals (Sweden)

    Wai Ting eChan

    2016-03-01

    Full Text Available In their initial stages of discovery, prokaryotic toxin-antitoxin (TA systems were confined to bacterial plasmids where they function to mediate the maintenance and stability of usually low- to medium-copy number plasmids through the post-segregational killing of any plasmid-free daughter cells that developed. Their eventual discovery as nearly ubiquitous and repetitive elements in bacterial chromosomes led to a wealth of knowledge and scientific debate as to their diversity and functionality in the prokaryotic lifestyle. Currently categorized into six different types designated types I – VI, type II TA systems are the best characterized. These generally comprised of two genes encoding a proteic toxin and its corresponding proteic antitoxin, respectively. Under normal growth conditions, the stable toxin is prevented from exerting its lethal effect through tight binding with the less stable antitoxin partner, forming a non-lethal TA protein complex. Besides binding with its cognate toxin, the antitoxin also plays a role in regulating the expression of the type II TA operon by binding to the operator site, thereby repressing transcription from the TA promoter. In most cases, full repression is observed in the presence of the TA complex as binding of the toxin enhances the DNA binding capability of the antitoxin. TA systems have been implicated in a gamut of prokaryotic cellular functions such as being mediators of programmed cell death as well as persistence or dormancy, biofilm formation, as defensive weapons against bacteriophage infections and as virulence factors in pathogenic bacteria. It is thus apparent that these antitoxins, as DNA-binding proteins, play an essential role in modulating the prokaryotic lifestyle whilst at the same time preventing the lethal action of the toxins under normal growth conditions, i.e., keeping the proverbial wolves at bay. In this review, we will cover the diversity and characteristics of various type II TA