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Sample records for purification system bomba

  1. Absorption heat pump integrated in an effluent purification system; Bomba de calor por absorcion integrada a un sistema de purificacion de efluentes

    Energy Technology Data Exchange (ETDEWEB)

    Santoyo, Socrates; Siqueiros, Javier; Heard, Christopher; Santoyo, Edgar [Instituto de Investigaciones Electricas, Cuernavaca (Mexico)

    1996-12-31

    The results derived of the integration of an absorption heat pump to an industrial effluents purification system, are presented. The advantages of these heat pumps with respect to heat pumps by mechanical compression of vapor, as well as the advantages in using absorption heat pumps in simple distillation systems, are mentioned. Finally, a description is made of the equipment designed and built, as well as the results obtained in a preliminary test. [Espanol] Se presentan los resultados derivados de la integracion de una bomba de calor por absorcion a un sistema de purificacion de efluentes industriales. Se mencionan las ventajas de este tipo de bombas de calor con respecto a las de calor por compresion mecanica de vapor, asi como las ventajas de usar bombas de calor en sistemas de destilacion simple. Finalmente, se describe el equipo disenado y construido, asi como los resultados obtenidos de una prueba preliminar.

  2. El sistema de inyección de combustible tipo bomba inyector UPS (Unit Pump System)

    OpenAIRE

    Tamayo Ramírez, Marlon Antonio; Hurtado Chiriboga, Hernán Mauricio

    2007-01-01

    El sistema de inyección de combustible electrónica diesel tipo bomba conducto inyector UPS (Unit Pump System), con bombas de inyección individual, es la continuación de los sistemas de inyección con bomba de inyección conjunta. Estas bombas llamadas bombas de inyección solidarias al bloque motor, se asignan individualmente a cada cilindro. En la bomba de inyección solidaria al bloque motor, la alta presión de combustible, se sigue produciendo según el principio de bomba de émbolo, como ...

  3. Heat pump for purification of geothermal brines; Bomba de calor para purificacion de salmuera geotermica

    Energy Technology Data Exchange (ETDEWEB)

    Santoyo-Gutierrez, S; Barragan-Reyes, R.M; Holland, F.A [Instituto de Investigaciones Electricas, Cuernavaca, Morelos (Mexico)]. E-mail: rmb@iie.org.mx

    2007-01-15

    Integrated use of geothermal resources is one of the most important goals for the future. Presently geothermal heat pumps offer two benefits: using heat from residual brines and converting these brines into very pure water. Designs and descriptions are presented of an experimental system to purify geothermal brines integrated to an adsorption heat-pump. The system was constructed and tested in the IIE (Institute for Electrical Research) facilities. During the experimental stage, pure water was obtained. Maximum capacity for pure water was 4.3 kg per hour, presenting an Actual Coefficient of Performance (COP)A of 1.4. The results are encouraging to project units at an industrial level for operating with geothermal and/or solar heat. [Spanish] El aprovechamiento integral de los recursos geotermicos en todas sus formas es una de las metas importantes a lograr en los proximos anos. Hoy en dia, el uso de las bombas de calor en la geotermia ofrece un doble beneficio: aprovechan el calor de los fluidos de desecho y tienen la capacidad de transformar la salmuera geotermica en agua de alta pureza. Se presenta el diseno y descripcion de un sistema experimental para purificacion de salmuera geotermica integrado a una bomba de calor por absorcion, el cual fue construido y probado en el Instituto de Investigaciones Electricas. En toda la etapa de experimentacion se obtuvo agua pura. La capacidad maxima alcanzada de produccion de agua pura de este sistema fue de 4.3 kg por hora, mostrando un rendimiento en terminos del Coeficiente Real de Rendimiento (COP)A de 1.4. Estos resultados se consideran alentadores para la proyeccion de unidades a escala industrial que puedan ser operadas con calor geotermico y/o solar.

  4. The Borexino purification system

    Science.gov (United States)

    Benziger, Jay

    2014-05-01

    Purification of 278 tons of liquid scintillator and 889 tons of buffer shielding for the Borexino solar neutrino detector is performed with a system of combined distillation, water extraction, gas stripping and filtration. The purification system removed K, U and Th by distillation of the pseudocumene solvent and the PPO fluor. Noble gases, Rn, Kr and Ar were removed by gas stripping. Distillation was also employed to remove optical impurities and reduce the attenuation of scintillation light. The success of the purification system has facilitated the first time real time detection of low energy solar neutrinos.

  5. Hybrid system: Heat pump-solar air dryer for grains; Sistema hibrido: bomba de calor - calentador solar de aire para el secado de productos agricolas

    Energy Technology Data Exchange (ETDEWEB)

    Soto Gomez, Willfredo [Instituto Tecnologico de Tijuana, Tijuana (Mexico); Ortega Herrera, Jose Angel [Instituto Politecnico Nacional, Mexico, D.F. (Mexico)

    2000-07-01

    Design, building, operation and evaluation energy wise of a hybrid experimental type, with heat pump, that uses no chloride, does not destroy the ozone layer. It is solar air dryer for grains. In this research we dry rice. It has tree systems: 1.- A mechanical compression heat pump, 2.- An air solar heater, and 3.- An agriculture products dryer. The drying capacity is 20 pounds of grain /day, with a median daily solar radiation. The costs is approximately U.S. $ 6 000.00. The heat pump used 22 refrigerant first, and now works with refrigerant SUVA 9000. This refrigerant will be available this year in the I.S., it is one of the ecological class that substitutes the chlorofluorocarbonates. [Spanish] Se disena, construye, opera, y evalua energeticamente, un sistema hibrido tipo experimental, con bomba de calor que utiliza refrigerante que no contiene cloro, y no destruye la capa de ozono y un calentador solar de aire, para secar granos. En este trabajo secamos arroz. Se compone de tres sistemas: 1.- Bomba de calor por compresion mecanica, 2.- Calentador solar de aire, 3.- Secador de productos agricolas. La capacidad de secado es de 10 Kilos de granos/dia promedio. Tiene un costo aproximado de $ 60 000. La bomba de calor utiliza refrigerante 22 en una primera generacion, y actualmente opera con un refrigerante SUVA 9000, en una segunda generacion, este refrigerante se comercializara en este ano, en la Union Americana, pertenece a la familia de los llamados refrigerantes ecologicos, sustitutos de los clorofluorocarbonados.

  6. Water Purification Systems

    Science.gov (United States)

    1994-01-01

    Clearwater Pool Technologies employs NASA-developed silver/copper ionization to purify turtle and dolphin tanks, cooling towers, spas, water recycling systems, etc. The pool purifier consists of a microcomputer to monitor water conditions, a pair of metallic electrodes, and a rheostat controller. Ions are generated by passing a low voltage current through the electrodes; the silver ions kill the bacteria, and the copper ions kill algae. This technology has found broad application because it offers an alternative to chemical disinfectants. It was originally developed to purify water on Apollo spacecraft. Caribbean Clear has been using NASA's silver ionization technology for water purification for more than a decade. Two new products incorporate advancements of the basic technology. One is the AquaKing, a system designed for areas with no source of acceptable drinking water. Another is the Caribbean Clear Controller, designed for commercial pool and water park applications where sanitizing is combined with feedback control of pH and an oxidizer, chlorine or bromine. The technology was originally developed to purify water on Apollo spacecraft.

  7. The SELEX Air Purification System

    Science.gov (United States)

    2010-01-07

    REPORT Final Report for the SELEX Air Purification System 14. ABSTRACT 16. SECURITY CLASSIFICATION OF: A new air purification technology ( SELEX ) was...developed and demonstrated. The SELEX system utilizes an array of electrospray wick aerosol sources for particle ionization and an electrostatic...precipitator for particle collection. The particle ionization process does not produce ozone and the SELEX technology provides a unique combination of

  8. Reverse osmosis water purification system

    Science.gov (United States)

    Ahlstrom, H. G.; Hames, P. S.; Menninger, F. J.

    1986-01-01

    A reverse osmosis water purification system, which uses a programmable controller (PC) as the control system, was designed and built to maintain the cleanliness and level of water for various systems of a 64-m antenna. The installation operates with other equipment of the antenna at the Goldstone Deep Space Communication Complex. The reverse osmosis system was designed to be fully automatic; with the PC, many complex sequential and timed logic networks were easily implemented and are modified. The PC monitors water levels, pressures, flows, control panel requests, and set points on analog meters; with this information various processes are initiated, monitored, modified, halted, or eliminated as required by the equipment being supplied pure water.

  9. Solid State Air Purification System Project

    Data.gov (United States)

    National Aeronautics and Space Administration — The purpose of this proposed research is to develop a new air purification system based on a liquid membrane, capable of purifying carbon dioxide from air in a far...

  10. Research and technological development on heat pumps in Mexico operating with geothermal energy; Investigacion y desarrollo tecnologico sobre bombas de calor en Mexico operando con energia geotermica

    Energy Technology Data Exchange (ETDEWEB)

    Garcia Gutierrez, Alfonso; Barragan Reyes, Rosa Maria; Arellano Gomez, Victor Manuel [Instituto de Investigaciones Electricas, Cuernavaca, Morelos (Mexico)

    2008-07-01

    The Instituto de Investigaciones Electricas (IIE) and the Comision Federal de Electricidad (CFE) carried out in the past an extensive work of research and development (R&D) on heat pumps (HP). The systems tried on include heat pumps by mechanical compression, thermal absorption and thermal transformers. This paper briefly describes the main aspects of R&D on heat pumps and presents a more detailed description of three of the main studies: a) a Heat Pump (HP) by mechanical compression water-water type, designed for brine purification, operating with low pressure geothermal steam at the geothermal field Los Azufres, Michoacan, Mexico; b) a HP by absorption for cooling and refrigeration, operating with ammoniac/water and low enthalpy geothermal energy, which was tested in the geothermal fields of Los Azufres, Michoacan and Cerro Prieto, Baja California, and c) a thermal transformer by absorption, named Heat Pump by Absorption Type 2, which was tested to evaluate the behavior of diverse ternary solutions as working fluids. To date, there are plans to install and test a geothermal heat pump (connected to the subsoil), in Cerro Prieto, Mexicali, Baja California, Mexico. [Spanish] El Instituto de Investigaciones Electricas (IIE) y la Comision Federal de Electricidad (CFE) realizaron un trabajo extenso de investigacion y desarrollo (I&D) sobre bombas de calor (BC) en el pasado. Los sistemas que se probaron incluyen bombas de calor por compresion mecanica, absorcion y transformadores termicos. Este trabajo describe brevemente los principales aspectos de I&D sobre bombas de calor y se da una descripcion mas detallada de tres de los principales estudios: a) una Bomba de Calor (BC) por compresion mecanica tipo agua-agua, disenada para purificacion de salmueras, operando con vapor geotermico de baja presion en el campo geotermico de Los Azufres, Michoacan; b) una BC por absorcion para enfriamiento y refrigeracion, operando con amoniaco/agua y energia geotermica de baja entalpia

  11. 21 CFR 876.5665 - Water purification system for hemodialysis.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Water purification system for hemodialysis. 876... SERVICES (CONTINUED) MEDICAL DEVICES GASTROENTEROLOGY-UROLOGY DEVICES Therapeutic Devices § 876.5665 Water purification system for hemodialysis. (a) Identification. A water purification system for hemodialysis is...

  12. Período de recuperação do investimento em bomba hidráulica de melhor rendimento em sistemas de bombeamento na tarifa horossazonal verde Investment recovery period in hydraulic pump of better efficiency in pumping systems in the green hourly seasonal tariff for irrigation

    Directory of Open Access Journals (Sweden)

    João L. Zocoler

    2011-04-01

    Full Text Available Neste trabalho, ajustou-se um modelo matemático para quantificar o efeito da variação do rendimento da bomba hidráulica na variação dos custos de um sistema de bombeamento, na estrutura tarifária horossazonal verde (subgrupo A4 e o tempo de recuperação do capital investido no equipamento de maior rendimento. Em seguida, o mesmo foi aplicado a um sistema de bombeamento para suprimento de um sistema de irrigação do tipo pivô central. As opções de rendimento da bomba hidráulica foram: 69,5% (bomba 1, 73% (bomba 2 e 78% (bomba 3, cujos custos de aquisição foram, respectivamente, R$ 6.176,00, R$ 8.479,00 e R$ 15.509,00. Os resultados da aplicação do modelo mostraram que: i a substituição da bomba 1 pela bomba 2 foi viável, sendo o período de recuperação de capital 3,4 anos; ii a substituição da bomba 1 pela bomba 3 foi viável, sendo o período de recuperação de capital 9,2 anos; iii a substituição da bomba 2 pela bomba 3 foi inviável, sendo o período de recuperação de capital 21,1 anos superior ao período de amortização do investimento na avaliação econômica, considerado 15 anos.In this study a mathematical model was adjusted to quantify the effect of the pump efficiency on cost variation of the pumping system in the green hourly seasonal tariff (subgroup A4 - irrigation and the investment recovery period by a more efficient pumping station. Afterwards, the same model was applied in a pumping system supplying a center pivot irrigation machine. The hydraulics pump efficiency options were: 69.5% (pump 1, 73% (pump 2 and 78% (pump 3, with acquisition costs of R$ 6,176.00, R$ 8,479.00 and R$ 15,509.00, respectively. The results of the model applied showed that: i the substitution of pump 1 for 2 was feasible, being the investment recovery period of 3.4 years; ii the substitution of bomb 1 for 3 was feasible, being the investment recovery period of 9.2 years; and iii the substitution of bomb 2 for 3 was unfeasible

  13. Intein-mediated purification system: mechanism and applications

    Institute of Scientific and Technical Information of China (English)

    Sarra setrerrahmane; Shuhua Tan

    2013-01-01

    The incorporation of self-cleaving protein elements into a variety of fusion-based purification systems; has been an important development in the area of recombinant protein purification. The self-cleaving capability of these tags has recently been combined with additional purification tags to generate novel and convenient protein purification methods. This review elucidates the properties of intein, the mechanism of the intein-based protein splicing and the progress of intein-based protein purification procedures, and recent advances in the applications of intein.

  14. Automated Water-Purification System

    Science.gov (United States)

    Ahlstrom, Harlow G.; Hames, Peter S.; Menninger, Fredrick J.

    1988-01-01

    Reverse-osmosis system operates and maintains itself with minimal human attention, using programmable controller. In purifier, membranes surround hollow cores through which clean product water flows out of reverse-osmosis unit. No chemical reactions or phase changes involved. Reject water, in which dissolved solids concentrated, emerges from outer membrane material on same side water entered. Flow controls maintain ratio of 50 percent product water and 50 percent reject water. Membranes expected to last from 3 to 15 years.

  15. A scintillator purification system for the Borexino solar neutrino detector

    Science.gov (United States)

    Benziger, J.; Cadonati, L.; Calaprice, F.; Chen, M.; Corsi, A.; Dalnoki-Veress, F.; Fernholz, R.; Ford, R.; Galbiati, C.; Goretti, A.; Harding, E.; Ianni, Aldo; Ianni, Andrea; Kidner, S.; Leung, M.; Loeser, F.; McCarty, K.; McKinsey, D.; Nelson, A.; Pocar, A.; Salvo, C.; Schimizzi, D.; Shutt, T.; Sonnenschein, A.

    2008-03-01

    Purification of the 278 tons of liquid scintillator and 889 tons of buffer shielding for the Borexino solar neutrino detector is performed with a system that combines distillation, water extraction, gas stripping, and filtration. This paper describes the principles of operation, design, and construction of that purification system, and reviews the requirements and methods to achieve system cleanliness and leak-tightness.

  16. Research on heat pumps in Mexico operating with geothermal energy and waste heat; Investigacion sobre bombas de calor en Mexico operando con energia geotermica y calor de desecho

    Energy Technology Data Exchange (ETDEWEB)

    Garcia-Gutierrez, A; Barragan-Reyes, R.M; Arellano-Gomez, V [Instituto de Investigaciones Electricas, Cuernavaca, Morelos (Mexico)]. E-mail: aggarcia@iie.org.mx

    2008-01-15

    The Instituto de Investigaciones Electricas and the Comision Federal de Electricidad have done research and development (R&D) on heat pumps (HP) in past years. Tested systems include mechanical compression, absorption and heat-transformers. The main R&D aspects on HP are briefly described, and also a more detailed description about three of the main studies is presented: (a) a mechanical compression HP of the water-water type operated with low-pressure geothermal steam at the Los Azufres; Mich., geothermal field, and designed for purification of brine; (b) an absorption HP for cooling and refrigeration operating with ammonia-water and low-enthalpy geothermal energy, which was tested in the Los Azufres and Cerro Prieto, BC, geothermal fields; and (c) a heat-transformer by absorption-Absorption Heat Pump Type II-tested to assess the performance of several ternary solutions as work fluids. Plans exist to install and test a geothermal heat pump at Cerro Prieto or Mexicali, BC. [Spanish] El Instituto de Investigaciones Electricas y la Comision Federal de Electricidad han realizado trabajo de investigacion y desarrollo (I&D) sobre bombas de calor (BC) en el pasado. Los sistemas probados incluyen compresion mecanica, absorcion y transformadores termicos. Este trabajo describe brevemente los principales aspectos de I&D sobre bombas de calor en forma general, y se da una descripcion mas detallada de tres de los principales estudios: (a) una BC por compresion mecanica tipo agua-agua disenada para purificacion de salmueras operando con vapor geotermico de baja presion en el campo geotermico de Los Azufres, Mich.; (b) una BC por absorcion para enfriamiento y refrigeracion operando con amoniaco-agua y energia geotermica de baja entalpia, la cual fue probada en los campos geotermicos de Los Azufres y Cerro Prieto, BC; y (c) un transformador termico por absorcion -llamado Bomba de Calor por Absorcion Tipo II--, el cual fue probado para evaluar el comportamiento de diversas

  17. Optimization of ground source heat pumps systems through the implementation of hybrid systems; Optimizacion de las instalaciones de bomba de calor geotermica mediante la implementacion de sistemas hibridos

    Energy Technology Data Exchange (ETDEWEB)

    Magraner, T.; Quilis, M. S.; Martinez, S.

    2009-07-01

    One of the fundamental aspects to consider in the design of a ground heat pump system is the heat balance in the ground since thermal saturation of the ground produces a decreasing of the performance of the system throughout its useful life. The hybrid geothermal system which combined geothermal heat pump system with other systems for generation or dissipation energy are very suitable for balancing the heat exchanger meters needed for the proper functioning of the system, important aspect to reduce costs and achieve attractive return periods of the initial investment. Energesis Ingenieria has developed and implemented in two office buildings, a design of hybrid systems based on the combination of a geothermal heat pump and air-condensed units (dry coolers) that can ensure energy efficiencies comparable to geothermal pure systems, reducing substantially the investment cost. (Author) 5 refs.

  18. Bomba de succión

    OpenAIRE

    Verdezoto Ganchozo, Fabricio Ludgardo; Verdezoto Ganchozo, Richard Fabianny; Yapur Auad, Miguel Eduardo

    2009-01-01

    El objetivo es implementar una unidad móvil que realice un trabajo de aspiración de manera silenciosa, para drenaje de líquidos en pacientes y que permita seleccionar dos niveles de succión, 120 y 90 mm Hg. Las bombas que usan motores eléctricos para generar succión generalmente son ruidosas, por lo que en este proyecto se desarrolla otro sistema para generar el vacío necesario para obtener la succión. El vacío será desarrollado por medio de un sistema cíclico de calentamiento de un resistor...

  19. Bomba de succión

    OpenAIRE

    Verdezoto Ganchozo, Fabricio Ludgardo; Verdezoto Ganchozo, Richard Fabianny; Yapur Auad, Miguel Eduardo

    2009-01-01

    El objetivo es implementar una unidad móvil que realice un trabajo de aspiración de manera silenciosa, para drenaje de líquidos en pacientes y que permita seleccionar dos niveles de succión, 120 y 90 mm Hg. Las bombas que usan motores eléctricos para generar succión generalmente son ruidosas, por lo que en este proyecto se desarrolla otro sistema para generar el vacío necesario para obtener la succión. El vacío será desarrollado por medio de un sistema cíclico de calentamiento de un resistor...

  20. A gênese da bomba

    Directory of Open Access Journals (Sweden)

    Joaquim Francisco de Carvalho

    2015-08-01

    Full Text Available O artigo traz à memória o desenvolvimento da física no século XX e relembra os cientistas que, direta ou indiretamente, na Europa e nos Estados Unidos, deram as suas contribuições para os programas nucleares de objetivos militares, que culminaram com as bombas de urânio e de plutônio que causaram a morte de centenas de milhares de pessoas inocentes, no Japão.

  1. A Scintillator Purification Plant and Fluid Handling System for SNO+

    CERN Document Server

    Ford, Richard J

    2015-01-01

    A large capacity purification plant and fluid handling system has been constructed for the SNO+ neutrino and double-beta decay experiment, located 6800 feet underground at SNOLAB, Canada. SNO+ is a refurbishment of the SNO detector to fill the acrylic vessel with liquid scintillator based on Linear Alkylbenzene (LAB) and 2 g/L PPO, and also has a phase to load natural tellurium into the scintillator for a double-beta decay experiment with 130Te. The plant includes processes multi-stage dual-stream distillation, column water extraction, steam stripping, and functionalized silica gel adsorption columns. The plant also includes systems for preparing the scintillator with PPO and metal-loading the scintillator for double-beta decay exposure. We review the basis of design, the purification principles, specifications for the plant, and the construction and installations. The construction and commissioning status is updated.

  2. Diseño de una bomba dosificadora

    OpenAIRE

    Rodríguez Borja, Manuel

    2015-01-01

    Una bomba dosificadora de líquidos pastosos es un dispositivo que facilita el llenado del mencionado producto que no alcanza la solidez en el envase elegido. Su utilización es clave en el proceso de paquetización debido a la difícil tarea de manipular ciertos elementos por el problema con sus densidades y composiciones. Diseñar una bomba dosificadora de líquidos pastosos; se trata de la labor a realizar en el proyecto. El diseño se ha realizado por completo en una empresa dedic...

  3. The effect of water purification systems on fluoride content of drinking water

    OpenAIRE

    Prabhakar A; Raju O; Kurthukoti A; Vishwas T

    2008-01-01

    Objective: The purpose of the present study was to determine the effect of different water purification systems on the fluoride content of drinking water and to compare the efficacy of these water purification systems in reducing the fluoride content. Materials and Methods: Five different water purification systems were tested in this study. They were reverse osmosis, distillation, activated carbon, Reviva ® , and candle filter. The water samples in the study were of two types, viz, bo...

  4. New research on bioregenerative air/water purification systems

    Science.gov (United States)

    Johnson, Anne H.; Ellender, R. D.; Watkins, Paul J.

    1991-01-01

    For the past several years, air and water purification systems have been developed and used. This technology is based on the combined activities of plants and microorganisms as they function in a natural environment. More recently, researchers have begun to address the problems associated with indoor air pollution. Various common houseplants are currently being evaluated for their abilities to reduce concentrations of volatile organic compounds (VOCS) such as formaldehyde and benzene. With development of the Space Exploration Initiative, missions will increase in duration, and problems with resupply necessitates implementation of regenerative technology. Aspects of bioregenerative technology have been included in a habitat known as the BioHome. The ultimate goal is to use this technology in conjunction with physicochemical systems for air and water purification within closed systems. This study continued the risk assessment of bioregenerative technology with emphasis on biological hazards. In an effort to evaluate the risk for human infection, analyses were directed at enumeration of fecal streptococci and enteric viruses with the BioHome waste water treatment system.

  5. Circulation and Purification in the LUX-ZEPLIN System Test

    Science.gov (United States)

    Alsum, Shaun; Lz Collaboration

    2016-03-01

    LZ is a dark-matter direct detection experiment whose detector is a two-phase TPC using approximately seven tons of active xenon as its scintillator. The xenon must have few electronegative impurities to ensure sufficient electron transport through the drift region. The LZ purification system is being prototyped in the LZ system test, a test platform located at SLAC using about 100kg of Xenon, which consists of gas circulation through a SAES getter. We utilize a dual-phase and a gas-phase heat exchanger to reduce needed cooling power. To achieve this circulation we employ an all metal seal triple diaphragm pump, also prototyped in the System Test. This talk will present early results from the system test as well as some baseline LZ designs. The LUX-ZEPLIN dark matter direct detection experiment.

  6. Development of an automated system for isolation and purification of humic substances

    NARCIS (Netherlands)

    Zomeren, van A.; Weij-Zuiver, van der E.; Comans, R.N.J.

    2008-01-01

    Characterization of humic substances (HS) in environmental samples generally involves labor-intensive and time-consuming isolation and purification procedures. In this paper, the development of an automated system for HS isolation and purification is described. The novelty of the developed system li

  7. Development of an automated system for isolation and purification of humic substances

    NARCIS (Netherlands)

    Zomeren, van A.; Weij-Zuiver, van der E.; Comans, R.N.J.

    2008-01-01

    Characterization of humic substances (HS) in environmental samples generally involves labor-intensive and time-consuming isolation and purification procedures. In this paper, the development of an automated system for HS isolation and purification is described. The novelty of the developed system

  8. DEVELOPMENT OF A NON-NOBLE METAL HYDROGEN PURIFICATION SYSTEM

    Energy Technology Data Exchange (ETDEWEB)

    Korinko, P; Kyle Brinkman, K; Thad Adams, T; George Rawls, G

    2008-11-25

    Development of advanced hydrogen separation membranes in support of hydrogen production processes such as coal gasification and as front end gas purifiers for fuel cell based system is paramount to the successful implementation of a national hydrogen economy. Current generation metallic hydrogen separation membranes are based on Pd-alloys. Although the technology has proven successful, at issue is the high cost of palladium. Evaluation of non-noble metal based dense metallic separation membranes is currently receiving national and international attention. The focus of the reported work was to develop a scaled reactor with a VNi-Ti alloy membrane to replace a production Pd-alloy tube-type purification/diffuser system.

  9. THE WATER PURIFICATION SYSTEM OF MACHINE-BUILDING COMPLEX FROM OIL-PRODUCTS

    Directory of Open Access Journals (Sweden)

    A. S. Panasugin

    2005-01-01

    Full Text Available The developed system of purification of sewage water from petroleum products allows to provide efficiency of purification up to the norms of PDK, and if necessary the devices can be used as a separate modules or their combinations.

  10. The controllability analysis of the purification system for heavy water reactors

    Energy Technology Data Exchange (ETDEWEB)

    Lee, K. D.; Cho, B. H.; Shin, C. H.; Kim, S. H. [KEPRI, Taejon (Korea, Republic of); Lee, Y. K.; Kim, K. U. [KHNP, Kyungju (Korea, Republic of)

    2001-10-01

    The heavy water reactor such as Wolsung No.1 and No.2 has a purification system to purify the reactor coolant. The control system regulates the coolant temperature to protect the ion exchanger. After the fuel exchanges of operating plant, the increase of the coolant pressure makes the purification temperature control difficult. In this paper, the controllability of the control dynamics of the purification system was analysed and the optimal parameters were proposed. To reduce the effects of the flow disturbance, the feedforward control structure was proposed and analysed.

  11. The effect of water purification systems on fluoride content of drinking water

    Directory of Open Access Journals (Sweden)

    Prabhakar A

    2008-03-01

    Full Text Available Objective: The purpose of the present study was to determine the effect of different water purification systems on the fluoride content of drinking water and to compare the efficacy of these water purification systems in reducing the fluoride content. Materials and Methods: Five different water purification systems were tested in this study. They were reverse osmosis, distillation, activated carbon, Reviva ® , and candle filter. The water samples in the study were of two types, viz, borewell water and tap water, these being commonly used by the people of Davangere City, Karnataka. The samples were collected before and after purification, and fluoride analysis was done using fluoride ion-specific electrode. Results: The results showed that the systems based on reverse osmosis, viz, reverse osmosis system and Reviva ® showed maximum reduction in fluoride levels, the former proving to be more effective than the latter; followed by distillation and the activated carbon system, with the least reduction being brought about by candle filter. The amount of fluoride removed by the purification system varied between the system and from one source of water to the other. Interpretation and Conclusion: Considering the beneficial effects of fluoride on caries prevention; when drinking water is subjected to water purification systems that reduce fluoride significantly below the optimal level, fluoride supplementation may be necessary. The efficacy of systems based on reverse osmosis in reducing the fluoride content of water indicates their potential for use as defluoridation devices.

  12. The effect of water purification systems on fluoride content of drinking water.

    Science.gov (United States)

    Prabhakar, A R; Raju, O S; Kurthukoti, A J; Vishwas, T D

    2008-03-01

    The purpose of the present study was to determine the effect of different water purification systems on the fluoride content of drinking water and to compare the efficacy of these water purification systems in reducing the fluoride content. Five different water purification systems were tested in this study. They were reverse osmosis, distillation, activated carbon, Reviva , and candle filter. The water samples in the study were of two types, viz, borewell water and tap water, these being commonly used by the people of Davangere City, Karnataka. The samples were collected before and after purification, and fluoride analysis was done using fluoride ion-specific electrode. The results showed that the systems based on reverse osmosis, viz, reverse osmosis system and Reviva showed maximum reduction in fluoride levels, the former proving to be more effective than the latter; followed by distillation and the activated carbon system, with the least reduction being brought about by candle filter. The amount of fluoride removed by the purification system varied between the system and from one source of water to the other. Considering the beneficial effects of fluoride on caries prevention; when drinking water is subjected to water purification systems that reduce fluoride significantly below the optimal level, fluoride supplementation may be necessary. The efficacy of systems based on reverse osmosis in reducing the fluoride content of water indicates their potential for use as defluoridation devices.

  13. Undulative induction electron accelerator for the waste and natural water purification systems

    CERN Document Server

    Kulish, Victor V; Gubanov, I V

    2001-01-01

    The project analysis of Undulative Induction Accelerator (EH - accelerator) for the waste and natural water purification systems is accomplished. It is shown that the use of the four-channel design of induction block and the standard set of auxiliary equipment (developed earlier for the Linear Induction Accelerators - LINACs) allow to construct commercially promising purification systems. A quality analysis of the accelerator is done and the optimal parameters are chosen taking into account the specific sphere of its usage.

  14. Purification of replication factors using insect and mammalian cell expression systems.

    Science.gov (United States)

    Uno, Shuji; You, Zhiying; Masai, Hisao

    2012-06-01

    Purification of factors for DNA replication in an amount sufficient for detailed biochemical characterization is essential to elucidating its mechanisms. Insect cell expression systems are commonly used for purification of the factors proven to be difficult to deal with in bacteria. We describe first the detailed protocols for purification of mammalian Mcm complexes including the Mcm2/3/4/5/6/7 heterohexamer expressed in insect cells. We then describe a convenient and economical system in which large-sized proteins and multi-factor complexes can be transiently overexpressed in human 293T cells and be rapidly purified in a large quantity. We describe various expression vectors and detailed methods for transfection and purification of various replication factors which have been difficult to obtain in a sufficient amount in other systems. Availability of efficient methods to overproduce and purify the proteins that have been challenging would facilitate the enzymatic analyses of the processes of DNA replication.

  15. Practice and design of the self-purification system for heavy metals-bearing contaminants

    Institute of Scientific and Technical Information of China (English)

    Qian Guangren

    2008-01-01

    Many minerals in nature have self-purification capacity to hold and stabilize deleterious contaminants into their lattice structures,which can be used for treatment of heavy metals-bearing contaminants.Hydrotalcite Layer Double Hy-droxide(LDH),tobermorite Calcium Silicate Hydrate(CSH)and apatite are ubiquitous minerals in nature,having higher geochemical stability and potential for binding and stabilizing heavy metals.Based on the elucidation of crystal structure property and self-purification principles of the three minerals above,this article discussed how to design the self-purification system of heavy metal-bearing contaminants.

  16. Purification of liquid metal systems with sodium coolant from oxygen using getters

    Science.gov (United States)

    Kozlov, F. A.; Konovalov, M. A.; Sorokin, A. P.

    2016-05-01

    For increasing the safety and economic parameters of nuclear power stations (NPSs) with sodium coolant, it was decided to install all systems contacting radioactive sodium, including purification systems of circuit I, in the reactor vessel. The performance and capacity of cold traps (CTs) (conventional element of coolant purification systems) in these conditions are limited by their volume. It was proposed to use hot traps (HTs) in circuit I for coolant purification from oxygen. It was demonstrated that, at rated parameters of the installation when the temperature of the coolant streamlining the getter (gas absorber) is equal to 550°C, the hot trap can provide the required coolant purity. In shutdown modes at 250-300°C, the performance of the hot trap is reduced by four orders of magnitude. Possible HT operation regimes for shutdown modes and while reaching rated parameters were proposed and analyzed. Basic attention was paid to purification modes at power rise after commissioning and accidental contamination of the coolant when the initial oxygen concentration in it reached 25 mln-1. It was demonstrated that the efficiency of purification systems can be increased using HTs with the getter in the form of a foil or granules. The possibility of implementing the "fast purification" mode in which the coolant is purified simultaneously with passing over from the shutdown mode to the rated parameters was substantiated.

  17. Fluidos, bombas e instalaciones hidráulicas

    OpenAIRE

    Heras Jiménez, Salvador Augusto de las

    2011-01-01

    A la portada: Universitat Politècnica de Catalunya. Barcelonatech Descripció del recurs: 2 de maig de 2012 Esta obra describe los principios fundamentales que rigen el funcionamiento de las bombas y las instalaciones hidráulicas más usuales, sus características operativas y los criterios de diseño y selección. El contenido del libro se estructura en tres grupos básicos. Los dos primeros temas tratan de la mecánica de los fluidos y sus propiedades: los principios y las leyes de conser...

  18. Development of RAP Tag, a Novel Tagging System for Protein Detection and Purification.

    Science.gov (United States)

    Fujii, Yuki; Kaneko, Mika K; Ogasawara, Satoshi; Yamada, Shinji; Yanaka, Miyuki; Nakamura, Takuro; Saidoh, Noriko; Yoshida, Kanae; Honma, Ryusuke; Kato, Yukinari

    2017-04-01

    Affinity tag systems, possessing high affinity and specificity, are useful for protein detection and purification. The most suitable tag for a particular purpose should be selected from many available affinity tag systems. In this study, we developed a novel affinity tag called the "RAP tag" system, which comprises a mouse antirat podoplanin monoclonal antibody (clone PMab-2) and the RAP tag (DMVNPGLEDRIE). This system is useful not only for protein detection in Western blotting, flow cytometry, and sandwich enzyme-linked immunosorbent assay, but also for protein purification.

  19. Design of the Helium Purifier for IHEP-ADS Helium Purification System

    CERN Document Server

    Jianqin, Zhang; Zhuo, Zhang; Rui, Ge

    2015-01-01

    Helium Purification System is an important sub-system in the Accelerator Driven Subcritical System of the Institute of High Energy Physics(IHEP ADS). The purifier is designed to work at the temperature of 77K. The purifier will work in a flow rate of 5g/s at 20MPa in continuous operation of 12 hours. The oil and moisture are removed by coalescing filters and a dryer, while nitrogen and oxygen are condensed by a phase separator and then adsorbed in several activated carbon adsorption cylinders. After purification, the purified helium has an impurity content of less than 5ppm.

  20. The importance of the ammonia purification process in ammonia-water absorption systems

    Energy Technology Data Exchange (ETDEWEB)

    Fernandez-Seara, Jose [Area de Maquinas y Motores Termicos, Escuela Tecnica Superior de Ingenieros Industriales, Universidad de Vigo, Campus Lagoas-Marcosende No. 9, 36200 Vigo (Spain)]. E-mail: jseara@uvigo.es; Sieres, Jaime [Area de Maquinas y Motores Termicos, Escuela Tecnica Superior de Ingenieros Industriales, Universidad de Vigo, Campus Lagoas-Marcosende No. 9, 36200 Vigo (Spain)

    2006-08-15

    Practical experience in working with ammonia-water absorption systems shows that the ammonia purification process is a crucial issue in order to obtain an efficient and reliable system. In this paper, the detrimental effects of the residual water content in the vapour refrigerant are described and quantified based on the system design variables that determine the effectiveness of the purification process. The study has been performed considering a single stage system with a distillation column with complete condensation. The ammonia purification effectiveness of the column is analysed in terms of the efficiencies in the stripping and rectifying sections and the reflux ratio. By varying the efficiencies from 0 to 1, systems with neither the rectifying nor stripping section, with either the rectifying or stripping section, or with both sections can be considered. The impact of the ammonia purification process on the absorption system performance is studied based on the column efficiencies and reflux ratio; and its effects on refrigerant concentration, system COP, system pressures and main system mass flow rates and concentrations are analysed. When the highest efficiency rectifying sections are used a combination of generation temperature and reflux ratio which leads to optimum COP values is found. The analysis covers different operating conditions with air and water cooled systems from refrigeration to air conditioning applications by changing the evaporation temperature. The importance of rectification in each kind of application is evaluated.

  1. Application of surface response analysis to the optimization of penicillin acylase purification in aqueous two-phase systems

    OpenAIRE

    2002-01-01

    Penicillin acylase purification from an Escherichia coli crude extract using PEG 3350 – sodium citrate aqueous two phase systems was optimized. An experimental design was used to evaluate the influence of PEG, sodium citrate and sodium chloride on the purification parameters. A central composite design was defined centred on the previously found conditions for highest purification from an osmotic shock extract. Mathematical models for the partition coefficient of protein and enzyme, balance o...

  2. Preparation and Purification of 125I With Neutron Irradiated Xenon in a Vacuum Circular system

    Institute of Scientific and Technical Information of China (English)

    MIAOZeng-xing; LIYu-cheng; YUNing-wen; WUJie; XIANGXue-qin; ZHAOXiu-yan

    2003-01-01

    This paper describes the preparation and purification of 125I with neutron irradiated xenon in a vacuum circular system, which is specially designed with an irradiate chamber set inside of the reactor and three decay chambers set outside of the reactor. The xenon is filled in this system and recurrently circulates between the irradiate chamber and the decay chambers during the reactor is operating.

  3. Bromelain purification through unconventional aqueous two-phase system (PEG/ammonium sulphate).

    Science.gov (United States)

    Coelho, D F; Silveira, E; Pessoa Junior, A; Tambourgi, E B

    2013-02-01

    This paper focuses on the feasibility of unconventional aqueous two-phase systems for bromelain purification from pineapple processing waste. The main difference in comparison with conventional systems is the integration of the liquid-liquid extraction technique with fractional precipitation, which can decrease the protein content with no loss of biological activity by removing of unwanted molecules. The analysis of the results was based on the response surface methodology and revealed that the use of the desirability optimisation methodology (DOM) was necessary to achieve higher purification factor values and greater bromelain recovery. The use of DOM yielded an 11.80-fold purification factor and 66.38 % biological activity recovery using poly(ethylene glycol) (PEG) with a molar mass of 4,000, 10.86 % PEG concentration (m/m) and 36.21 % saturation of ammonium sulphate.

  4. Procedimiento para evaluar y seleccionar bombas centrífugas

    Directory of Open Access Journals (Sweden)

    Rafael Pérez-Barreto

    2004-03-01

    Full Text Available Se analiza la velocidad específica, aplicando el análisis dimensional y la teoría de semejanza y su valor en función de los parámetros utilizados para su determinación. Se fundamenta el uso de la velocidad específica obtenida para gastos unitarios del caudal y la carga del modelo medidos en m3/s y mH2O respectivamente, cuando la velocidad de rotación se expresa en r/min. Se propone y ejemplifica un procedimiento rápido y práctico para evaluar comparativamente la calidad del diseño o de su ejecución, usando la velocidad específica y el rendimiento nominales. La conocida relación entre estos parámetros ha sido actualizada y se aplica en la evaluación y selección de bombas centrífugas. Este trabajo forma parte de una serie de artículos que analizan la esencia física de la velocidad específica, su origen y las principales imprecisiones que aparecen en la literatura, y pretende generalizar el uso de la velocidad específica recomendada, para facilitar a los usuarios —en particular a los de la industria del níquel—, evaluar, en primera instancia, la calidad del diseño o de la fabricación de bombas centrífugas, así como la confiabilidad de los datos aportados por el suministrador, y entre fabricantes, a fin de aumentar la calidad del diseño o controlar la de fabricación y con ello aumentar su competitividad.

  5. Características peculiares de la operación de bombas rotodinámicas en paralelo

    OpenAIRE

    Yaset Martínez Valdés; Félix Riaño Valle

    2010-01-01

    Este es el primero de varios artículos que se presentarán sobre el tema de las bombas rotodinámicas operando en paralelo. En el se hace primeramente un breve resumen de las características de funcionamiento y comportamiento hidráulico de las bombas acopladas en paralelo. Se presenta el método de las bombas virtuales, un procedimiento sencillo y práctico para la solución de problemas de bombas conectadas en paralelo, cuando las condiciones de instalación y/o características propias de las bomb...

  6. Características peculiares de la operación de bombas rotodinámicas en paralelo

    OpenAIRE

    Yaset Martínez Valdés; Félix Riaño Valle

    2010-01-01

    Este es el primero de varios artículos que se presentarán sobre el tema de las bombas rotodinámicas operando en paralelo. En el se hace primeramente un breve resumen de las características de funcionamiento y comportamiento hidráulico de las bombas acopladas en paralelo. Se presenta el método de las bombas virtuales, un procedimiento sencillo y práctico para la solución de problemas de bombas conectadas en paralelo, cuando las condiciones de instalación y/o características propias de las bomb...

  7. Diagnosis of feed water, condensate and circulation pumps in electric power plants; Diagnostico de bombas de agua de alimentacion, condensado y circulacion en centrales termoelectricas

    Energy Technology Data Exchange (ETDEWEB)

    Colin Castellanos, Carlos [Instituto de Investigaciones Electricas, Cuernavaca (Mexico)

    1991-12-31

    This article encompasses the analysis and the diagnosis of the pump`s performance that belong to the feed water, condensate and circulation systems of a fossil fuel power plant (FFPP). For this analysis pressure, temperature and flow data were collected by means of field installed instrumentation, as well as these pumps` motors current consumption and voltage values. Later on, the capacity and the pump efficiency are calculated and compared with the design values, to obtain the actual performance of the equipment with the aid of their characteristic curves (Q vs {Delta}H, Q vs {pi}, etc.). [Espanol] Este articulo comprende el analisis y el diagnostico de comportamiento de las bombas, las cuales forman parte de los sistemas de agua de alimentacion, condensado y circulacion de una central termoelectrica (CT). Para el analisis se recopilan datos de presion, temperatura y flujo de la instrumentacion instalada en campo, asi como de los valores de consumo de corriente y de voltaje en los motores de dichas bombas. Posteriormente, se calcula la capacidad y la eficiencia de las bombas en operacion real y se comparan con los valores de diseno, para obtener el comportamiento real del equipo con ayuda de las curvas caracteristicas (Q vs {Delta}H, Q vs {pi}, etcetera).

  8. Período de recuperação do investimento em bomba hidráulica de melhor rendimento em sistemas de bombeamento na tarifa horossazonal verde Investment recovery period in hydraulic pump of better efficiency in pumping systems in the green hourly seasonal tariff for irrigation

    OpenAIRE

    Zocoler, João L.; Hernandez,Fernando B. T.; Saad,João C. C.; Cruz,Raimundo L.

    2011-01-01

    Neste trabalho, ajustou-se um modelo matemático para quantificar o efeito da variação do rendimento da bomba hidráulica na variação dos custos de um sistema de bombeamento, na estrutura tarifária horossazonal verde (subgrupo A4) e o tempo de recuperação do capital investido no equipamento de maior rendimento. Em seguida, o mesmo foi aplicado a um sistema de bombeamento para suprimento de um sistema de irrigação do tipo pivô central. As opções de rendimento da bomba hidráulica foram: 69,5% (bo...

  9. 21 CFR 884.6170 - Assisted reproduction water and water purification systems.

    Science.gov (United States)

    2010-04-01

    ... HUMAN SERVICES (CONTINUED) MEDICAL DEVICES OBSTETRICAL AND GYNECOLOGICAL DEVICES Assisted Reproduction Devices § 884.6170 Assisted reproduction water and water purification systems. (a) Identification... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Assisted reproduction water and water...

  10. Field Testing of a Small Water Purification System for Non-PRASA Rural Communities

    Science.gov (United States)

    Small, rural communities typically do not have adequate water purification systems to sustain their life quality and residents are exposed to pathogens present in drinking water. In Puerto Rico (PR), approximately 4% of the population does not have access to drinking water provi...

  11. Field Testing of a Small Water Purification System for Non-PRASA Rural Communities

    Science.gov (United States)

    Small, rural communities typically do not have adequate water purification systems to sustain their life quality and residents are exposed to pathogens present in drinking water. In Puerto Rico (PR), approximately 4% of the population does not have access to drinking water provi...

  12. Hemólise em circulação extracorpórea: estudo comparativo entre bomba de rolete e bomba centrífuga Hemolysis in extracorporeal circulation: a comparative study between roller and centrifugal pumps

    Directory of Open Access Journals (Sweden)

    Paulo M Pêgo-Fernandes

    1989-12-01

    Full Text Available O uso de bomba centrífuga como suporte circulatório para pacientes em choque cardiogênico, após a realização de cirurgia cardíaca e como suporte para pacientes com falência cardíaca que estão aguardando doação para transplante cardíaco, tem sido progressivamente ampliado. Alguns centros utilizam a bomba centrífuga em circulação extracorpórea de rotina, como substituto do rolete arterial,. No INCOR, operamos dois grupos de pacientes triarteriais submetidos a revascularização do miocárdio, operados pelo mesmo cirurgião, com o mesmo método de proteção miocárdica (cardioplegia cristalóide, hipotermia sistêmica a 28ºC e tópica com soro fisiológico. Todos os parâmetros dos dois grupos foram sem diferença estatística no pré-operatório: idade, sexo, superfície corpórea e parâmetros hematológicos. Foram operados 27 pacientes consecutivos e divididos, alternadamente, em 13 pacientes com bomba centrífuga e 14 com rolete arterial. O oxigenador utilizado em todos foi o de bolhas da Macchi. O perfusionista foi sempre o mesmo. O tempo de perfusão médio foi de 105 minutos no Grupo 1 (rolete e 103 minutos no Grupo 2 (bomba centrífuga. Analisamos os seguintes parâmetros: haptoglobina (HP, tempo de tromboplastina parcial ativada (TTPA, tempo de trombina (TT e número e plaquetas pré e pós circulação extracorpórea e, comparando-se os dois grupos, não houve diferença estatística significante entre eles, nos diversos parâmetros. Concluímos que, para circulação extracorpórea com duração habitual, não há diferença hematológica no uso da bomba centrífuga em relação ao rolete arterial.The utilization of centrifugal pumps as circulatory support in patients with cardiogenic shock after cardiac surgery and as support in patients waiting for cardiac transplant has been progressively extended. Some centers utilize the centrifugal pump in routine extracorporeal circulation as a substitute for roller pump. At

  13. Subsurface architecture of Las Bombas volcano circular structure (Southern Mendoza, Argentina) from geophysical studies

    Science.gov (United States)

    Prezzi, Claudia; Risso, Corina; Orgeira, María Julia; Nullo, Francisco; Sigismondi, Mario E.; Margonari, Liliana

    2017-08-01

    The Plio-Pleistocene Llancanelo volcanic field is located in the south-eastern region of the province of Mendoza, Argentina. This wide back-arc lava plateau, with hundreds of monogenetic pyroclastic cones, covers a large area behind the active Andean volcanic arc. Here we focus on the northern Llancanelo volcanic field, particularly in Las Bombas volcano. Las Bombas volcano is an eroded, but still recognizable, scoria cone located in a circular depression surrounded by a basaltic lava flow, suggesting that Las Bombas volcano was there when the lava flow field formed and, therefore, the lava flow engulfed it completely. While this explanation seems reasonable, the common presence of similar landforms in this part of the field justifies the need to establish correctly the stratigraphic relationship between lava flow fields and these circular depressions. The main purpose of this research is to investigate Las Bombas volcano 3D subsurface architecture by means of geophysical methods. We carried out a paleomagnetic study and detailed topographic, magnetic and gravimetric land surveys. Magnetic anomalies of normal and reverse polarity and paleomagnetic results point to the occurrence of two different volcanic episodes. A circular low Bouguer anomaly was detected beneath Las Bombas scoria cone indicating the existence of a mass deficit. A 3D forward gravity model was constructed, which suggests that the mass deficit would be related to the presence of fracture zones below Las Bombas volcano cone, due to sudden degassing of younger magma beneath it, or to a single phreatomagmatic explosion. Our results provide new and detailed information about Las Bombas volcano subsurface architecture.

  14. Hamiltonian purification

    Energy Technology Data Exchange (ETDEWEB)

    Orsucci, Davide [Scuola Normale Superiore, I-56126 Pisa (Italy); Burgarth, Daniel [Department of Mathematics, Aberystwyth University, Aberystwyth SY23 3BZ (United Kingdom); Facchi, Paolo; Pascazio, Saverio [Dipartimento di Fisica and MECENAS, Università di Bari, I-70126 Bari (Italy); INFN, Sezione di Bari, I-70126 Bari (Italy); Nakazato, Hiromichi; Yuasa, Kazuya [Department of Physics, Waseda University, Tokyo 169-8555 (Japan); Giovannetti, Vittorio [NEST, Scuola Normale Superiore and Istituto Nanoscienze-CNR, I-56126 Pisa (Italy)

    2015-12-15

    The problem of Hamiltonian purification introduced by Burgarth et al. [Nat. Commun. 5, 5173 (2014)] is formalized and discussed. Specifically, given a set of non-commuting Hamiltonians (h{sub 1}, …, h{sub m}) operating on a d-dimensional quantum system ℋ{sub d}, the problem consists in identifying a set of commuting Hamiltonians (H{sub 1}, …, H{sub m}) operating on a larger d{sub E}-dimensional system ℋ{sub d{sub E}} which embeds ℋ{sub d} as a proper subspace, such that h{sub j} = PH{sub j}P with P being the projection which allows one to recover ℋ{sub d} from ℋ{sub d{sub E}}. The notions of spanning-set purification and generator purification of an algebra are also introduced and optimal solutions for u(d) are provided.

  15. Purification of uranothorite solid solutions from polyphase systems

    Energy Technology Data Exchange (ETDEWEB)

    Clavier, Nicolas, E-mail: nicolas.clavier@icsm.fr [ICSM, UMR 5257 CEA/CNRS/UM2/ENSCM, Site de Marcoule – Bât. 426, BP 17171, 30207 Bagnols/Cèze cedex (France); Szenknect, Stéphanie; Costin, Dan Tiberiu; Mesbah, Adel; Ravaux, Johann [ICSM, UMR 5257 CEA/CNRS/UM2/ENSCM, Site de Marcoule – Bât. 426, BP 17171, 30207 Bagnols/Cèze cedex (France); Poinssot, Christophe [CEA/DEN/DRCP/DIR, Site de Marcoule – Bât. 400, BP 17171, 30207 Bagnols/Cèze cedex (France); Dacheux, Nicolas [ICSM, UMR 5257 CEA/CNRS/UM2/ENSCM, Site de Marcoule – Bât. 426, BP 17171, 30207 Bagnols/Cèze cedex (France)

    2013-10-15

    Graphical abstract: Display Omitted -- Highlights: •Purification of Th{sub 1−x}U{sub x}SiO{sub 4} uranothorites from oxide mixture was investigated. •Repetition of centrifugation steps was discarded due to poor recovery yields. •Successive washings in acid and basic media allowed the elimination of oxide secondary phases. •Structural and microstructural characterization of the purified samples was provided. -- Abstract: The mineral coffinite, nominally USiO{sub 4}, and associated Th{sub 1−x}U{sub x}SiO{sub 4} uranothorite solid solutions are of great interest from a geochemical point of view and in the case of the direct storage of spent nuclear fuels. Nevertheless, they clearly exhibit a lack in the evaluation of their thermodynamic data, mainly because of the difficulties linked with their preparation as pure phases. This paper thus presents physical and chemical methods aiming to separate uranothorite solid solutions from oxide additional phases such as amorphous SiO{sub 2} and nanometric crystallized Th{sub 1−y}U{sub y}O{sub 2}. The repetition of centrifugation steps envisaged in first place was rapidly dropped due to poor recovery yields, to the benefit of successive washings in acid then basic media. Under both static and dynamic flow rates (i.e. low or high rate of leachate renewal), ICP-AES (Inductively Coupled Plasma – Atomic Emission Spectroscopy) analyses revealed the systematic elimination of Th{sub 1−y}U{sub y}O{sub 2} in acid media and of SiO{sub 2} in basic media. Nevertheless, two successive steps were always needed to reach pure samples. On this basis, a first cycle performed in static conditions was chosen to eliminate the major part of the accessory phases while a second one, in dynamic conditions, allowed the elimination of the residual impurities. The complete purification of the samples was finally evidenced through the characterization of the samples by the means of PXRD (Powder X-Ray Diffraction), SEM (Scanning Electron

  16. Advanced Water Purification System for In Situ Resource Utilization Project

    Science.gov (United States)

    Anthony, Stephen M.

    2014-01-01

    A main goal in the field of In Situ Resource Utilization is to develop technologies that produce oxygen from regolith to provide consumables to an extratrrestrial outpost. The processes developed reduce metal oxides in the regolith to produce water, which is then electrolyzed to produce oxygen. Hydrochloric and hydrofluoric acids are byproducts of the reduction processes, which must be removed to meet electrolysis purity standards. We previously characterized Nation, a highly water selective polymeric proton-exchange membrane, as a filtrtion material to recover pure water from the contaminated solution. While the membranes successfully removed both acid contaminants, the removal efficiency of and water flow rate through the membranes were not sufficient to produce large volumes of electrolysis-grade water. In the present study, we investigated electrodialysis as a potential acid removable technique. Our studies have show a rapid and significant reduction in chloride and fluoride concentrations in the feed solution, while generating a relatively small volume of concentrated waste water. Electrodialysis has shown significant promise as the primary separation technique in ISRU water purification processes.

  17. Advanced Water Purification System for In Situ Resource Utilization

    Science.gov (United States)

    Anthony, Stephen M.; Jolley, Scott T.; Captain, James G.

    2013-01-01

    A main goal in the field of In Situ Resource Utilization is to develop technologies that produce oxygen from regolith to provide consumables to an extraterrestrial outpost. The processes developed reduce metal oxides in the regolith to produce water, which is then electrolyzed to produce oxygen. Hydrochloric and hydrofluoric acids are byproducts of the reduction processes, which must be removed to meet electrolysis purity standards. We previously characterized Nation, a highly water selective polymeric proton-exchange membrane, as a filtration material to recover pure water from the contaminated solution. While the membranes successfully removed both acid contaminants, the removal efficiency of and water flow rate through the membranes were not sufficient to produce large volumes of electrolysis-grade water. In the present study, we investigated electrodialysis as a potential acid removal technique. Our studies have shown a rapid and significant reduction in chloride and fluoride concentrations in the feed solution, while generating a relatively small volume of concentrated waste water. Electrodialysis has shown significant promise as the primary separation technique in ISRU water purification processes.

  18. Características peculiares de la operación de bombas rotodinámicas en paralelo

    Directory of Open Access Journals (Sweden)

    Yaset Martínez Valdés

    2010-01-01

    Full Text Available Este es el primero de varios artículos que se presentarán sobre el tema de las bombas rotodinámicas operando en paralelo. En el se hace primeramente un breve resumen de las características de funcionamiento y comportamiento hidráulico de las bombas acopladas en paralelo. Se presenta el método de las bombas virtuales, un procedimiento sencillo y práctico para la solución de problemas de bombas conectadas en paralelo, cuando las condiciones de instalación y/o características propias de las bombas acopladas, no son las mismas.

  19. Efficiency in removing pollutants by constructed wetland purification systems in Poland.

    Science.gov (United States)

    Samecka-Cymerman, A; Stepien, D; Kempers, A J

    2004-02-27

    The aim of this study was to compare the efficiency between Phragmites communis, Salix viminalis, and Populus canadensis in removing the heavy metals Al, Ba, Mn, Ni, Sr, V, Zn, Cd, Cu, and Pb and the eutrophying macroelements phosphate, nitrate, nitrite, ammonia, chloride, sulfate, Ca, Mg, K, and Fe from sewage in subsurface flow constructed wetlands in Poland. The effectiveness of the sewage treatment system was higher in summer compared to winter for the removal of (1) all heavy metals, phosphate (P) and mineral nitrogen (N) for all of species, (2) sulfates (S) for Phragmites and Salix, (3) iron (Fe) for Salix, and (4) chloride (Cl) for Salix and Populus. Analysis of variance indicated that there was no significant difference between the purifications systems in phytoremediation of Mn; so all species were equally effective (99%, prob. level 0.001). The Salix wetland system was most effective in purification of water and removal of macroelements (24-82% in summer, 10-80% in winter with Fe 97%), Cd (58-71%), V (100%), and Zn (84-92%). The Phragmites system was most effective in purification and removal of Al (81-97%), Ba (70-95%), Pb (64-81%), and Sr (24-51%), while in the case of Cu (49-60%) and Ni (55-67%) the Populus wetland system proved most effective. The outflowing water of the wetlands contained elements in amounts exceeding the admissible levels as established for unpolluted water both in winter and summer. Therefore the effectiveness of the observed phytoremediation systems in this study was not sufficient alone to remove these elements and can be considered as a supplemental tool in purification of sewage.

  20. Simulació d'una turbina-bomba amb CFD

    OpenAIRE

    González Fuentes, Raúl

    2016-01-01

    En aquest projecte es durà a terme l’estudi d’una turbina/bomba francis, de la central hidràulica de Sallente (Pallars Jussà, Lleida), utilitzant les eines del programa CFD (Computational Fluid Dynamics). S’utilitzarà un model numèric per tal de comprovar les prestacions, interaccions i fenòmens fluidodinamics de la bomba/turbina a estudiar. El projecte només es centrarà en l’estudi del distribuïdor de la turbina/bomba francis. Es determinarà la interacció entre els àleps del distribuïdor ...

  1. Guide to the use of pond systems in South Africa for the purification of raw and partially treated sewage

    CSIR Research Space (South Africa)

    Meiring, PGJ

    1968-01-01

    Full Text Available Some of the effluents from the sewage purification systems covered by this report show quite marked seasonal variations. No assurance can be given that the effluents would meet promulgated standards from season to season. The effluent from...

  2. Metodologia para Obtenção de Rotores Radiais Otimizados nos Modos Bomba e Turbina Utilizando Critérios de Carregamento Hidrodinâmico.

    OpenAIRE

    FAJARDO, Lady Castellanos

    2013-01-01

    Este trabalho apresenta uma metodologia para obtenção de rotores radiais otimizados nos modos bomba e turbina utilizando critérios de carregamento hidrodinâmico. Em geral, um rotor de bomba é projetado para operar somente no modo bomba e um rotor de turbina hidráulica é projetado para operar apenas no modo turbina. Uma turbomáquina hidráulica reversível, denominada de bomba-turbina, deve operar de forma eficiente tanto no modo bomba como no modo turbina. Para essa finalidade, um roto...

  3. Performance Assessment of SOFC Systems Integrated with Bio-Ethanol Production and Purification Processes

    Directory of Open Access Journals (Sweden)

    Sumittra Charojrochkul

    2010-03-01

    Full Text Available The overall electrical efficiencies of the integrated systems of solid oxide fuel cell (SOFC and bio-ethanol production with purification processes at different heat integration levels were investigated. The simulation studies were based on the condition with zero net energy. It was found that the most suitable operating voltage is between 0.7 and 0.85 V and the operating temperature is in the range from 973 to 1173 K. For the effect of percent ethanol recovery, the optimum percent ethanol recovery is at 95%. The most efficient case is the system with full heat integration between SOFC and bio-ethanol production and purification processes with biogas reformed for producing extra hydrogen feed for SOFC which has the overall electrical efficiency = 36.17%. However more equipment such as reformer and heat exchangers are required and this leads to increased investment cost.

  4. El sistema de inyección diesel electrónico bomba - inyector (UIS)

    OpenAIRE

    Hurtado Guzhñay, Diego Fernando; Plaza Espinoza, Alfredo Manuel

    2007-01-01

    En el capítulo I se halla detallado la descripción de todo el sistema, en donde se indican las principales características entre las que destacan: bomba de inyección individual para cada cilindro del motor, sistema de inyección directa, accionamiento mecánico y eléctrico, forma de la leva con dos perfiles para generar la alta presión. Para establecer los componentes se hace una división en dos sistemas el de baja presión: filtro de combustible, separador de agua, bomba de combustible (aliment...

  5. Dialysate purification after introduction of automated hot water disinfection system to central dialysis fluid delivery system.

    Science.gov (United States)

    Ogawa, Tomonari; Matsuda, Akihiko; Yamaguchi, Yumiko; Sasaki, Yusuke; Kanayama, Yuki; Maeda, Tadaaki; Noiri, Chie; Hasegawa, Hajime; Matsumura, Osamu; Mitarai, Tetsuya

    2012-01-01

    Most dialysis clinics in Japan have mainly adopted the central dialysis fluid delivery system (CDDS) to provide constant treatment to many patients. Chemical disinfection is the major maintenance method of the CDDS. Our clinic introduced an automated hot water disinfection system that used the heat conduction effect to disinfect a reverse osmosis (RO) device and dialysis fluid supply equipment. Endotoxin level and the amount of viable bacteria often showed abnormal values before introduction of this system. After its introduction, weekly disinfection resulted in endotoxin levels and the amount of viable bacteria lower than measurement sensitivity. In hot water disinfection, water heated to 90°C in the RO tank flows into the dialysis fluid supply equipment. The maximum temperature inside the tank of the supply equipment is 86.3°C. (We confirmed that the temperature was maintained at 80°C or more for 10 minutes or more during the monitoring.) Dialysate purification was maintained even after introduction of the automated hot water disinfection system and the dialysate could be supplied stably by the CDDS. Therefore, this disinfection system might be very useful in terms of both cost and safety, and can be used for dialysis treatment of multiple patients.

  6. Final LDRD report :ultraviolet water purification systems for rural environments and mobile applications.

    Energy Technology Data Exchange (ETDEWEB)

    Banas, Michael Anthony; Crawford, Mary Hagerott; Ruby, Douglas Scott; Ross, Michael P.; Nelson, Jeffrey Scott; Allerman, Andrew Alan; Boucher, Ray

    2005-11-01

    We present the results of a one year LDRD program that has focused on evaluating the use of newly developed deep ultraviolet LEDs in water purification. We describe our development efforts that have produced an LED-based water exposure set-up and enumerate the advances that have been made in deep UV LED performance throughout the project. The results of E. coli inactivation with 270-295 nm LEDs are presented along with an assessment of the potential for applying deep ultraviolet LED-based water purification to mobile point-of-use applications as well as to rural and international environments where the benefits of photovoltaic-powered systems can be realized.

  7. Purification of dialysis water in the central dialysis fluid delivery system in Japan: a prospective observation study.

    Science.gov (United States)

    Uchino, Junji; Kawasaki, Tadayuki

    2009-01-01

    Whereas the main measure of dialysis fluid purity is endotoxin (ET) activity in Japan, it is the viability count in Western countries. Because of this difference, little information is available concerning dialysis fluid purity determined in terms of viability count in Japan. Under these circumstances a fact-finding investigation was planned and conducted concerning dialysis fluid purity to demonstrate the effectiveness of dialysis fluid purification measures. 93 medical institutions are equipped with the central dialysis fluid delivery system (CDDS) unique to Japan. Almost all medical institutions surveyed have achieved the purification level of ultrapure dialysis fluid after ETRF, but the methods of ETRF use and management widely vary with each institution so that early validation of the methods of evaluation of ET inhibition and system management is in urgent need. It is also important that simple universal microbial monitoring and purification procedures be diffused far and wide as suggested by the Purification Guidelines proposed by us.

  8. Hemólise em circulação extracorpórea: estudo comparativo entre bomba de rolete e bomba centrífuga

    OpenAIRE

    Pêgo-Fernandes,Paulo M; Miura,Flávio; Higa,Sérgio S; Moreira, Luiz Felipe P.; Dallan,Luís Alberto; Dalton A. F. Chamone; Sérgio Almeida de OLIVEIRA; Stolf,Noedir A. G.; Jatene,Adib D

    1989-01-01

    O uso de bomba centrífuga como suporte circulatório para pacientes em choque cardiogênico, após a realização de cirurgia cardíaca e como suporte para pacientes com falência cardíaca que estão aguardando doação para transplante cardíaco, tem sido progressivamente ampliado. Alguns centros utilizam a bomba centrífuga em circulação extracorpórea de rotina, como substituto do rolete arterial,. No INCOR, operamos dois grupos de pacientes triarteriais submetidos a revascularização do miocárdio, oper...

  9. Estimates of the economic and environmental impacts of the use of turbine pumps in an isolated system in Brazil; Estimativas dos impactos economicos e ambientais do uso de bombas funcionando como turbina em um sistema isolado no Brasil

    Energy Technology Data Exchange (ETDEWEB)

    Cardoso, R.B.; Nogueira, L.A.H. [Universidade Federal de Itajuba (EXCEN/UNIFEI), MG (Brazil). Centro de Excelencia em Eficiencia Energetica

    2009-07-01

    Due to the environmental impacts caused by the use of primary sources from fossil fuels for generating electricity, renewable energy is gaining prominence in the world energy scene. In Brazil, particularly for isolated systems, the use of pumps functioning as hydraulic turbines - PFT is already a reality. In addition to care for a low demands systems, the PAT, have economic advantages and environment with respect to conventional sources of electric power generation. In this sense, this study estimates the economic and environmental impacts of the use of PFT in a Brazilian isolate region (North region), using with reference the use of diesel generators. According to the estimates has a potential use for hydraulic power generation of 16.5 kW and the option of using a PFT in comparison of a diesel generator would provide an internal rate of return on the order of 1000% and reductions in emissions of greenhouse gases by 79.5 tCO{sub 2} years.

  10. Design of the Helium Purifier for IHEP-ADS Helium Purification System

    OpenAIRE

    2015-01-01

    Helium Purification System is an important sub-system in the Accelerator Driven Subcritical System of the Institute of High Energy Physics(IHEP ADS). The purifier is designed to work at the temperature of 77K. The purifier will work in a flow rate of 5g/s at 20MPa in continuous operation of 12 hours. The oil and moisture are removed by coalescing filters and a dryer, while nitrogen and oxygen are condensed by a phase separator and then adsorbed in several activated carbon adsorption cylinders...

  11. Development of a new hydrogen purification system by using hydrogen absorbing alloy for generator cooling; Suiso kyuzo gokin riyo hatsudenkinai suiso jundo kojo system no kaihatsu

    Energy Technology Data Exchange (ETDEWEB)

    Haruki, N.; Sato, J.; Kogi, T.; Nishimura, Y. [Kansai Electric Power Co., Inc., Osaka (Japan); Takeda, H. [Japan Steel works Ltd., Tokyo (Japan)] Fujita, T. [Mitsubishi Electric Corp., Tokyo (Japan)

    1997-05-20

    Hydrogen absorbing alloys have a number of useful functions, such as energy conversion, hydrogen storage and purification. As an application to separation and purification of hydrogen, we have developed a new hydrogen purification system by using a hydrogen absorbing alloy for generator cooling. For demonstration testing with an actual machine, a hydrogen recovery and purification device using 120kg of alloy was manufactured and installed on No.5 turbine-synchronous generator at Himeji No.2 power station. This device is designed to improve the purity of the hydrogen gas in generator containing impurities such as nitrogen and oxygen. The test results tell that the purity of the hydrogen gas in the generator can be enhanced from 98% to 99.9% and maintained at this level under continuous operation. An application of the hydrogen purification system is expected to decrease the generator`s windage loss, resulting higher generator efficiency. 2 refs., 18 figs.

  12. Biofilm bacterial communities in urban drinking water distribution systems transporting waters with different purification strategies.

    Science.gov (United States)

    Wu, Huiting; Zhang, Jingxu; Mi, Zilong; Xie, Shuguang; Chen, Chao; Zhang, Xiaojian

    2015-02-01

    Biofilm formation in drinking water distribution systems (DWDS) has many adverse consequences. Knowledge of microbial community structure of DWDS biofilm can aid in the design of an effective control strategy. However, biofilm bacterial community in real DWDS and the impact of drinking water purification strategy remain unclear. The present study investigated the composition and diversity of biofilm bacterial community in real DWDSs transporting waters with different purification strategies (conventional treatment and integrated treatment). High-throughput Illumina MiSeq sequencing analysis illustrated a large shift in the diversity and structure of biofilm bacterial community in real DWDS. Proteobacteria, Firmicutes, Bacteroidetes, Actinobacteria, Nitrospirae, and Cyanobacteria were the major components of biofilm bacterial community. Proteobacteria (mainly Alphaproteobacteria, Betaproteobacteria, and Gammaproteobacteria) predominated in each DWDS biofilm, but the compositions of the dominant proteobacterial classes and genera and their proportions varied among biofilm samples. Drinking water purification strategy could shape DWDS biofilm bacterial community. Moreover, Pearson's correlation analysis indicated that Actinobacteria was positively correlated with the levels of total alkalinity and dissolved organic carbon in tap water, while Firmicutes had a significant positive correlation with nitrite nitrogen.

  13. Bomba, terremoto ou neve: o que para Jerusalém?

    Directory of Open Access Journals (Sweden)

    Leopoldo O. C. de Oliveira

    2013-10-01

    Full Text Available Este artigo contrasta fatos cotidianos ocorridos em Jerusalém, como ataques à bomba, terremotos e neve, com as configurações literárias da cidade, tentando auferir sua importância para o sentimento de pertença étnica e nacional do Israel contemporâneo.

  14. Two-phase aqueous micellar systems: an alternative method for protein purification

    Directory of Open Access Journals (Sweden)

    Rangel-Yagui C. O.

    2004-01-01

    Full Text Available Two-phase aqueous micellar systems can be exploited in separation science for the extraction/purification of desired biomolecules. This article reviews recent experimental and theoretical work by Blankschtein and co-workers on the use of two-phase aqueous micellar systems for the separation of hydrophilic proteins. The experimental partitioning behavior of the enzyme glucose-6-phosphate dehydrogenase (G6PD in two-phase aqueous micellar systems is also reviewed and new results are presented. Specifically, we discuss very recent work on the purification of G6PD using: i a two-phase aqueous micellar system composed of the nonionic surfactant n-decyl tetra(ethylene oxide (C10E4, and (ii a two-phase aqueous mixed micellar system composed of C10E4 and the cationic surfactant decyltrimethylammonium bromide (C10TAB. Our results indicate that the two-phase aqueous mixed (C10E4/C10TAB micellar system can improve significantly the partitioning behavior of G6PD relative to that observed in the two-phase aqueous C10E4 micellar system.

  15. A cost-effective ELP-intein coupling system for recombinant protein purification from plant production platform.

    Directory of Open Access Journals (Sweden)

    Li Tian

    Full Text Available BACKGROUND: Plant bioreactor offers an efficient and economical system for large-scale production of recombinant proteins. However, high cost and difficulty in scaling-up of downstream purification of the target protein, particularly the common involvement of affinity chromatography and protease in the purification process, has hampered its industrial scale application, therefore a cost-effective and easily scale-up purification method is highly desirable for further development of plant bioreactor. METHODOLOGY/PRINCIPAL FINDINGS: To tackle this problem, we investigated the ELP-intein coupling system for purification of recombinant proteins expressed in transgenic plants using a plant lectin (PAL with anti-tumor bioactivity as example target protein and rice seeds as production platform. Results showed that ELP-intein-PAL (EiP fusion protein formed novel irregular ER-derived protein bodies in endosperm cells by retention of endogenous prolamins. The fusion protein was partially self-cleaved in vivo, but only self-cleaved PAL protein was detected in total seed protein sample and deposited in protein storage vacuoles (PSV. The in vivo uncleaved EiP protein was accumulated up to 2-4.2% of the total seed protein. The target PAL protein could be purified by the ELP-intein system efficiently without using complicated instruments and expensive chemicals, and the yield of pure PAL protein by the current method was up to 1.1 mg/g total seed protein. CONCLUSION/SIGNIFICANCE: This study successfully demonstrated the purification of an example recombinant protein from rice seeds by the ELP-intein system. The whole purification procedure can be easily scaled up for industrial production, providing the first evidence on applying the ELP-intein coupling system to achieve cost-effective purification of recombinant proteins expressed in plant bioreactors and its possible application in industry.

  16. Polonium purification

    Energy Technology Data Exchange (ETDEWEB)

    Baker, J.D.

    1996-09-01

    Three processes for the purification of {sup 210}Po from irradiated bismuth targets are described. Safety equipment includes shielded hotcells for the initial separation from other activation products, gloveboxes for handling the volatile and highly toxic materials, and provisions for ventilation. All chemical separations must be performed under vacuum or in inerted systems. Two of the processes require large amounts of electricity; the third requires vessels made from exotic materials.

  17. PURIFICATION OF COBALT ANOLYTE USING THE NOVEL SOLVENT EXTRACTION SYSTEM

    Institute of Scientific and Technical Information of China (English)

    Y.F. Shen; W.Y. Xue; W. Y. Niu

    2003-01-01

    In present research, a novel extractant system (D2EHPA + naphthenic acid +pyridine-ester) was used to purify cobalt anolyte and a simulated industrial produc-tion were carried out. This novel extraction system can extract Cu and/or Ni againstCo from chloride medium solutions at pH range of 2.5-4.5. About 2g/l nickel and0.2g/l copper were removed from the cobalt chloride anolyte containing about 100g/lcobalt and 200g/l chloride ions respectively, the raffinate contains nickel and copperless than 0.03g/l and 0. 0003g/l respectively and can be used to electrolyze high-puritycobalt. About 5.5t cobalt anolyte was purified in the simulation industrial experimentand kilogram quantities of cobalt of 99.98% purity and about 95% recovery have beenproduced.

  18. DOMESTIC WASTEWATER PURIFICATION IN UPFLOW BIOFILM SYSTEM WITH DIFFUSED AERATION

    Directory of Open Access Journals (Sweden)

    A.Mesdaghinia

    1984-08-01

    Full Text Available The objective of this research was to conduct a bench scale study of fixed activated sludge treating domestic sewage. Two different units employing diffused aeration with plastic and aluminum media were studied in four separate phases. Data indicated that the system could produce a high quality effluent without any requirements for sludge recycling through the system. Suspended solids concentrations of 3-6 mg/1, BOD5 concentrations of 4-12 mg/1 and COD concentrations of 35-45 mg/1 were found in the effluent with wastewater retentions ranging from 3-15 hours, whereas an indication of nitrification was observed in higher detention periods. As far as the type of media was concerned, the plastic and aluminum media did not differ significantly once the microbes had grown on the media.

  19. Water purification through vacuum system; Purificacion de agua bajo vacio

    Energy Technology Data Exchange (ETDEWEB)

    Armenta-Deu, C.

    2004-07-01

    Fresh water production through vacuum systems are today a reasonable option at a much lower cost than tray conventional units, also based on evaporation-condensation process. The use of simple devices such as vacuum ejectors allows to reduce pressure down to 5 kPa at a very low cost, only 7. The requirement of having a constant water flow to reduce pressure has been easily solved using a close circuit and a low power pump which is powered by solar energy. The energy cost has been reduced dramatically, as the system operates at a very reduced temperature, 45 degree Celsius, and even as low as 35 degree Celsius, what causes a much lower energy requirement. The results obtained during the tests have shown that is possible to save up to 230 kJ per litre of fresh water, and up to 40 W per l/h. The system is fully compatible with thermal solar collectors of low temperature, and can be electrically powered by a solar panel of low power. (Author)

  20. Increase of COP for heat transformer in water purification systems. Part II - Without increasing heat source temperature

    Energy Technology Data Exchange (ETDEWEB)

    Romero, R.J. [Centro de Investigacion en Ingenieria y Ciencias Aplicadas, Universidad Autonoma del Estado de Morelos, Av. Universidad 1001, Col. Chamilpa, C.P. 62209 Cuernavaca, Morelos (Mexico)]. E-mail: rosenberg@uaem.mx; Siqueiros, J. [Centro de Investigacion en Ingenieria y Ciencias Aplicadas, Universidad Autonoma del Estado de Morelos, Av. Universidad 1001, Col. Chamilpa, C.P. 62209 Cuernavaca, Morelos (Mexico); Huicochea, A. [Posgrado en Ingenieria y Ciencias Aplicadas, Universidad Autonoma del Estado de Morelos, Av. Universidad 1001, Col. Chamilpa, C.P. 62209 Cuernavaca, Morelos (Mexico)

    2007-04-15

    The integration of a water purification system allows a heat transformer to increase the actual coefficient of performance, by the reduction of the amount of heat supplied by unit of heat. A new defined COP called COP{sub WP} is proposed for the present system, which considers the fraction of heat recycled. Simulation with proven software compares the performance of the modeling of an absorption heat transformer for water purification (AHTWP) operating with water/lithium bromide, as working fluid-absorbent pair. Plots of enthalpy-based coefficients of performance (COP{sub ET}) and water purification coefficient of performance (COP{sub WP}) are shown against absorber temperature for several thermodynamic operating conditions. The results showed that the proposed (AHTWP) system is capable of increasing the original value of COP{sub ET} up to 1.6 times its original value by recycling energy from a water purification system. The proposed COP{sub WP} allows increments for COP values from any experimental data for water purification or for any other distillation system integrated to a heat transformer, regardless of actual COP{sub A} value or working fluid-absorbent pair.

  1. Increase of COP for heat transformer in water purification systems. Pt. 2 - Without increasing heat source temperature

    Energy Technology Data Exchange (ETDEWEB)

    Romero, R.J.; Siqueiros, J. [Centro de Investigacion en Ingenieria y Ciencias Aplicadas, Universidad Autonoma del Estado de Morelos, Av. Universidad 1001, Col. Chamilpa, C.P. 62209 Cuernavaca, Morelos (Mexico); Huicochea, A. [Posgrado en Ingenieria y Ciencias Aplicadas, Universidad Autonoma del Estado de Morelos, Av. Universidad 1001, Col. Chamilpa, C.P. 62209 Cuernavaca, Morelos (Mexico)

    2007-04-15

    The integration of a water purification system allows a heat transformer to increase the actual coefficient of performance, by the reduction of the amount of heat supplied by unit of heat. A new defined COP called COP{sub WP} is proposed for the present system, which considers the fraction of heat recycled. Simulation with proven software compares the performance of the modeling of an absorption heat transformer for water purification (AHTWP) operating with water/lithium bromide, as working fluid-absorbent pair. Plots of enthalpy-based coefficients of performance (COP{sub ET}) and water purification coefficient of performance (COP{sub WP}) are shown against absorber temperature for several thermodynamic operating conditions. The results showed that the proposed (AHTWP) system is capable of increasing the original value of COP{sub ET} up to 1.6 times its original value by recycling energy from a water purification system. The proposed COP{sub WP} allows increments for COP values from any experimental data for water purification or for any other distillation system integrated to a heat transformer, regardless of actual COP{sub A} value or working fluid-absorbent pair. (author)

  2. Membrane-based systems for carbon capture and hydrogen purification

    Energy Technology Data Exchange (ETDEWEB)

    Berchtold, Kathryn A [Los Alamos National Laboratory

    2010-11-24

    This presentation describes the activities being conducted at Los Alamos National Laboratory to develop carbon capture technologies for power systems. This work is aimed at continued development and demonstration of a membrane based pre- and post-combustion carbon capture technology and separation schemes. Our primary work entails the development and demonstration of an innovative membrane technology for pre-combustion capture of carbon dioxide that operates over a broad range of conditions relevant to the power industry while meeting the US DOE's Carbon Sequestration Program goals of 90% CO{sub 2} capture at less than a 10% increase in the cost of energy services. Separating and capturing carbon dioxide from mixed gas streams is a first and critical step in carbon sequestration. To be technically and economically viable, a successful separation method must be applicable to industrially relevant gas streams at realistic temperatures and pressures as well as be compatible with large gas volumes. Our project team is developing polymer membranes based on polybenzimidazole (PBI) chemistries that can purify hydrogen and capture CO{sub 2} at industrially relevant temperatures. Our primary objectives are to develop and demonstrate polymer-based membrane chemistries, structures, deployment platforms, and sealing technologies that achieve the critical combination of high selectivity, high permeability, chemical stability, and mechanical stability all at elevated temperatures (> 150 C) and packaged in a scalable, economically viable, high area density system amenable to incorporation into an advanced Integrated Gasification Combined-Cycle (IGCC) plant for pre-combustion CO{sub 2} capture. Stability requirements are focused on tolerance to the primary synthesis gas components and impurities at various locations in the IGCC process. Since the process stream compositions and conditions (temperature and pressure) vary throughout the IGCC process, the project is focused on

  3. Hydrogen Purification and Recycling for an Integrated Oxygen Recovery System Architecture

    Science.gov (United States)

    Abney, Morgan B.; Greenwood, Zachary; Wall, Terry; Nur, Mononita; Wheeler, Richard R., Jr.; Preston, Joshua; Molter, Trent

    2016-01-01

    The United States Atmosphere Revitalization life support system on the International Space Station (ISS) performs several services for the crew including oxygen generation, trace contaminant control, carbon dioxide (CO2) removal, and oxygen recovery. Oxygen recovery is performed using a Sabatier reactor developed by Hamilton Sundstrand, wherein CO2 is reduced with hydrogen in a catalytic reactor to produce methane and water. The water product is purified in the Water Purification Assembly and recycled to the Oxygen Generation Assembly (OGA) to provide O2 to the crew. This architecture results in a theoretical maximum oxygen recovery from CO2 of approx.54% due to the loss of reactant hydrogen in Sabatier-produced methane that is currently vented outside of ISS. Plasma Pyrolysis Assembly (PPA) technology, developed by Umpqua Research Company, provides the capability to further close the Atmosphere Revitalization oxygen loop by recovering hydrogen from Sabatier-produced methane. A key aspect of this technology approach is the need to purify the hydrogen from the PPA product stream which includes acetylene, unreacted methane and byproduct water and carbon monoxide. In 2015, four sub-scale hydrogen separation systems were delivered to NASA for evaluation. These included two electrolysis single-cell hydrogen purification cell stacks developed by Sustainable Innovations, LLC, a sorbent-based hydrogen purification unit using microwave power for sorbent regeneration developed by Umpqua Research Company, and a LaNi4.6Sn0.4 metal hydride produced by Hydrogen Consultants, Inc. Here we report the results of these evaluations to-date, discuss potential architecture options, and propose future work.

  4. Hydrogen Purification and Recycling for an Integrated Oxygen Recovery System Architecture

    Science.gov (United States)

    Abney, Morgan B.; Greenwood, Zachary; Wall, Terry; Miller, Lee; Wheeler, Ray

    2016-01-01

    The United States Atmosphere Revitalization life support system on the International Space Station (ISS) performs several services for the crew including oxygen generation, trace contaminant control, carbon dioxide (CO2) removal, and oxygen recovery. Oxygen recovery is performed using a Sabatier reactor developed by Hamilton Sundstrand, wherein CO2 is reduced with hydrogen in a catalytic reactor to produce methane and water. The water product is purified in the Water Purification Assembly and recycled to the Oxygen Generation Assembly (OGA) to provide O2 to the crew. This architecture results in a theoretical maximum oxygen recovery from CO2 of approximately 54% due to the loss of reactant hydrogen in Sabatier-produced methane that is currently vented outside of ISS. Plasma Methane Pyrolysis technology (PPA), developed by Umpqua Research Company, provides the capability to further close the Atmosphere Revitalization oxygen loop by recovering hydrogen from Sabatier-produced methane. A key aspect of this technology approach is to purify the hydrogen from the PPA product stream which includes acetylene, unreacted methane and byproduct water and carbon monoxide. In 2015, four sub-scale hydrogen separation systems were delivered to NASA for evaluation. These included two electrolysis single-cell hydrogen purification cell stacks developed by Sustainable Innovations, LLC, a sorbent-based hydrogen purification unit using microwave power for sorbent regeneration developed by Umpqua Research Company, and a LaNi4.6Sn0.4 metal hydride produced by Hydrogen Consultants, Inc. Here we report the results of these evaluations, discuss potential architecture options, and propose future work.

  5. Water Collection Purification System: Identifying CF Capabilities and Requirements, and Assessing off-the-Shelf Purification Systems

    Science.gov (United States)

    2006-08-01

    media Ohio Pure Water Co Sand and silica dioxide of different grain sizes Birm media filter Ohio Pure Water Co Specific resin for iron when water does...Terminator filters Ohio Pure Water Co Same than Birm filter but with air injection system to add oxygen in the mixture Nitrate filter Ohio Pure Water Co...media 1,000-4,000 Birm media filter 1,000-2,000 Manganese greensand filter 1,000-2,700 Terminator filters 800-1,000 Nitrate filter

  6. Analysis of exosome purification methods using a model liposome system and tunable-resistive pulse sensing

    Science.gov (United States)

    Lane, Rebecca E.; Korbie, Darren; Anderson, Will; Vaidyanathan, Ramanathan; Trau, Matt

    2015-01-01

    Exosomes are vesicles which have garnered interest due to their diagnostic and therapeutic potential. Isolation of pure yields of exosomes from complex biological fluids whilst preserving their physical characteristics is critical for downstream applications. In this study, we use 100 nm-liposomes from 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and cholesterol as a model system as a model system to assess the effect of exosome isolation protocols on vesicle recovery and size distribution using a single-particle analysis method. We demonstrate that liposome size distribution and ζ-potential are comparable to extracted exosomes, making them an ideal model for comparison studies. Four different purification protocols were evaluated, with liposomes robustly isolated by three of them. Recovered yields varied and liposome size distribution was unaltered during processing, suggesting that these protocols do not induce particle aggregation. This leads us to conclude that the size distribution profile and characteristics of vesicles are stably maintained during processing and purification, suggesting that reports detailing how exosomes derived from tumour cells differ in size to those from normal cells are reporting a real phenomenon. However, we hypothesize that larger particles present in most purified exosome samples represent co-purified contaminating non-exosome debris. These isolation techniques are therefore likely nonspecific and may co-isolate non-exosome material of similar physical properties.

  7. Purification of C-phycocyanin from Spirulina platensis in aqueous two-phase systems using an experimental design

    Directory of Open Access Journals (Sweden)

    Francine Silva Antelo

    2015-02-01

    Full Text Available C-phycocyanin from Spirulina platensis was purified in aqueous two-phase systems (ATPS of polyethylene glycol (PEG/potassium phosphate, varying the molar mass of the PEG. Results using a full factorial design showed that an increase in the concentration of salt and decrease in the concentration of PEG caused an increment in the purification factor for all the ATPS studied. Optimization of the conditions of the purification was studied using a central composite rotatable design for each molar mass of PEG. The ATPS composed of 7% (w/w PEG 1500 or 4% (w/w PEG 8000 (g/gmol and 23 or 22.5% (w/w of phosphate resulted a purification factor of 1.6-fold for C-phycocyanin, with total and 57% recovery, respectively. Process conditions were optimized for the purification factor for the system with PEG 1500. The ATPS with 4% (w/w PEG 4000 or 4% (w/w PEG 6000 and 21% (w/w phosphate resulted purification factors of 2.1 and 2.2-fold, recovering 100% and 73.5%, respectively of C-phycocyanin in the top phase.

  8. A vector system for ABC transporter-mediated secretion and purification of recombinant proteins in Pseudomonas species.

    Science.gov (United States)

    Ryu, Jaewook; Lee, Ukjin; Park, Jiye; Yoo, Do-Hyun; Ahn, Jung Hoon

    2015-03-01

    Pseudomonas fluorescens is an efficient platform for recombinant protein production. P. fluorescens has an ABC transporter secreting endogenous thermostable lipase (TliA) and protease, which can be exploited to transport recombinant proteins across the cell membrane. In this study, the expression vector pDART was constructed by inserting tliDEF, genes encoding the ABC transporter, along with the construct of the lipase ABC transporter recognition domain (LARD), into pDSK519, a widely used shuttle vector. When the gene for the target protein was inserted into the vector, the C-terminally fused LARD allowed it to be secreted through the ABC transporter into the extracellular medium. After secretion of the fused target protein, the LARD containing a hydrophobic C terminus enabled its purification through hydrophobic interaction chromatography (HIC) using a methyl-Sepharose column. Alkaline phosphatase (AP) and green fluorescent protein (GFP) were used to validate the expression, export, and purification of target proteins by the pDART system. Both proteins were secreted into the extracellular medium in P. fluorescens. In particular, AP was secreted in several Pseudomonas species with its enzymatic activity in extracellular media. Furthermore, purification of the target protein using HIC yielded some degree of AP and GFP purification, where AP was purified to almost a single product. The pDART system will provide greater convenience for the secretory production and purification of recombinant proteins in Gram-negative bacteria, such as Pseudomonas species.

  9. Expression, purification, and bioactivity of GST-fused v-Src from a bacterial expression system

    Institute of Scientific and Technical Information of China (English)

    GONG Xing-guo; JI Jing; XIE Jie; ZHOU Yuan; ZHANG Jun-yan; ZHONG Wen-tao

    2006-01-01

    v-Src is a non-receptor protein tyrosine kinase involved in many signal transduction pathways and closely related to the activation and development of cancers. We present herethe expression, purification, and bioactivity of a GST (glutathione S-transferase)-fused v-Src from a bacterial expression system. Different culture conditions were examined in an isopropyl β-D-thiogalactopyranoside (IPTG)-regulated expression, and the fused protein was purified using GSH (glutathione) affinity chromatography. ELISA (enzyme-linked immunosorbent assay) was employed to determine the phosphorylation kinase activity of the GST-fused v-Src. This strategy seems to be more promising than the insect cell system or other eukaryotic systems employed in earlier Src expression.

  10. Hyperentanglement purification for two-photon six-qubit quantum systems

    Science.gov (United States)

    Wang, Guan-Yu; Liu, Qian; Deng, Fu-Guo

    2016-09-01

    Recently, two-photon six-qubit hyperentangled states were produced in experiment and they can improve the channel capacity of quantum communication largely. Here we present a scheme for the hyperentanglement purification of nonlocal two-photon systems in three degrees of freedom (DOFs), including the polarization, the first-longitudinal-momentum, and the second-longitudinal-momentum DOFs. Our hyperentanglement purification protocol (hyper-EPP) is constructed with two steps resorting to parity-check quantum nondemolition measurement on the three DOFs and swap gates, respectively. With these two steps, the bit-flip errors in the three DOFs can be corrected efficiently. Also, we show that using swap gates is a universal method for hyper-EPP in the polarization DOF and multiple-longitudinal-momentum DOFs. The implementation of our hyper-EPP is assisted by nitrogen-vacancy centers in optical microcavities, which could be achieved with current techniques. It is useful for long-distance high-capacity quantum communication with two-photon six-qubit hyperentanglement.

  11. Effect of charcoal on water purification

    OpenAIRE

    Suzuki, Hirotaka; Kawahigashi, Tatsuo

    2014-01-01

    [Abstract] A natural basin system purifies water through self-purification, but the water pollution load of a river might exceed its self-purification capacity. Charcoal, which is used for other uses aside from heating, such as air purification, was evaluated experimentally for water quality purification. The experiment described herein is based on simple water quality measurements. Some experimentally obtained results are discussed.

  12. Precisión en flujo de infusión programado de bombas de nutrición enteral

    Directory of Open Access Journals (Sweden)

    Juan Ignacio Padilla-Cuadra

    2008-09-01

    Full Text Available Justificación y objetivo: Se evaluó in vitro la precisión en el flujo de nutrición enteral en bombas peristálticas de nutrición enteral con el fin de determinar si ésta puede ser una variable que cause errores en la administración de la fórmula. Materiales y Métodos: Se evaluaron 13 bombas de infusión enteral del tipo peristáltico mediante la aplicación de un modelo de laboratorio que simulaba la administración a un paciente. Se usó para todos los casos una fórmula estándar diluida de la manera recomendada por el fabricante. Con un volumen meta de 240 mL. Se programó en cada bomba un flujo de infusión de 40 mL por hora. Para cada caso según el tiempo de infusión, se calculó el flujo de infusión real y a partir de la diferencia se calculó el porcentaje de error. Resultados: En 12 de las 13 bombas se detectó un error por exceso que fue de 7.5% hasta 52.5%. Solo una bomba, de marca distinta a todas las demás demostró un error de 0 mL/h con respecto a lo programado. Ninguna bomba mostró un déficit en el volumen de infusión. Conclusión: En la mayoría de las bombas evaluadas se detectó un porcentaje de error por exceso que supera lo estipulado por el fabricante.

  13. Development of concept for concurrent biocide generation and water system purification. [with application to Skylab water tanks

    Science.gov (United States)

    1974-01-01

    An attempt was made to construct an electrochemical system, using iodine, for water purification in Skylab. Data cover measurements of iodine production rates, effect of electrode size and geometry on iodine production rates, and feasibility of using stainless steels as reference electrodes.

  14. Bomba de infusão de insulina em diabetes melito tipo 1 Insulin pump therapy in type 1 diabetes mellitus

    Directory of Open Access Journals (Sweden)

    Raphael Del Roio Liberatore Jr.

    2006-08-01

    Full Text Available OBJETIVO: Rever a experiência com a utilização da bomba de infusão de insulina em crianças e adolescentes, a fim de orientar o pediatra quanto às suas indicações e complicações. FONTE DOS DADOS : Foi realizada revisão sistemática de artigos publicados em literatura que abordassem a utilização da bomba de infusão de insulina, suas indicações, complicações e resposta ao tratamento. Dessa forma, todos os artigos publicados entre 1995 e 2005 foram resgatados através do levantamento em banco de dados MEDLINE e LILACS. As palavras-chave utilizadas foram: insulin pumps, type 1 diabetes mellitus e diabetes mellitus. Foram separados os artigos que, além disso, versassem sobre o assunto na faixa etária descrita SÍNTESE DOS DADOS: O uso da bomba de infusão de insulina em pacientes com diabetes melito tipo 1 não é uma necessidade para todos os pacientes, visto que, com tratamentos intensivos, os resultados conseguidos são muito parecidos, em termos de hemoglobina glicada e de controle de complicações a médio e longo prazo. No entanto, a bomba permite um maior conforto ao paciente, no sentido de que ele não precisa ficar tão restrito a horários rígidos de refeição e pode levar uma vida com melhor qualidade. Um primeiro requisito para quem pretenda usar a bomba é adaptar-se a aparelhos conectados ao corpo e a ter uma rotina de monitorização glicêmica rigorosa, pois, sem isso, as vantagens da bomba serão anuladas. As complicações, com os avanços tecnológicos de que dispomos atualmente, são muito infreqüentes. O custo, no entanto, é maior que nos tratamentos convencionais. CONCLUSÃO: Com a evolução das bombas de infusão e dos monitores de glicemia, incluindo sistemas de monitorização contínua, abre-se caminho para as "bombas inteligentes", e estaremos com um verdadeiro "pâncreas artificial", que pode mesmo ser implantado no paciente, permitindo uma vida com todas as regalias de uma pessoa não diab

  15. Fusion tags for protein solubility, purification and immunogenicity in Escherichia coli: The novel Fh8 system

    Directory of Open Access Journals (Sweden)

    Sofia eCosta

    2014-02-01

    Full Text Available Proteins are now widely produced in diverse microbial cell factories. The Escherichia coli is still the dominant host for recombinant protein production but, as a bacterial cell, it also has its issues: the aggregation of foreign proteins into insoluble inclusion bodies is perhaps the main limiting factor of the E. coli expression system. Conversely, E. coli benefits of cost, ease of use and scale make it essential to design new approaches directed for improved recombinant protein production in this host cell.With the aid of genetic and protein engineering novel tailored-made strategies can be designed to suit user or process requirements. Gene fusion technology has been widely used for the improvement of soluble protein production and/or purification in E. coli, and for increasing peptide’s immunogenicity as well. New fusion partners are constantly emerging and complementing the traditional solutions, as for instance, the Fh8 fusion tag that has been recently studied and ranked among the best solubility enhancer partners. In this review, we provide an overview of current strategies to improve recombinant protein production in E. coli, including the key factors for successful protein production, highlighting soluble protein production, and a comprehensive summary of the latest available and traditionally-used gene fusion technologies. A special emphasis is given to the recently discovered Fh8 fusion system that can be used for soluble protein production, purification and immunogenicity in E. coli. The number of existing fusion tags will probably increase in the next few years, and efforts should be taken to better understand how fusion tags act in E. coli. This knowledge will undoubtedly drive the development of new tailored-made tools for protein production in this bacterial system.

  16. Application of mobile blood purification system in the treatment of acute renal failure dog model in the field environment

    Directory of Open Access Journals (Sweden)

    Zhi-min ZHANG

    2014-01-01

    Full Text Available Objective To evaluate the stability, safety and efficacy of mobile blood purification system in the treatment of acute renal failure dog model in the field environment. Methods The acute renal failure model was established in 4 dogs by bilateral nephrectomy, which was thereafter treated with the mobile blood purification system. The evaluation of functional index of the mobile blood purification system was performed after a short-time (2 hours and conventional (4 hours dialysis treatment. Results The mobile blood purification system ran stably in the field environment at a blood flow of 150-180ml/min, a dialysate velocity of 2000ml/h, a replacement fluid velocity of 2000ml/h, and ultrafiltration rate of 100-200ml/h. All the functions of alarming system worked well, including static upper limit alarm of ultrafiltration pressure (>100 mmHg, upper limit alarm of ambulatory arterial pressure (>400mmHg, upper limit alarm of ambulatory venous pressure (>400mmHg, bubble alarm of vascular access, bubble alarm during the infusion of solutions, pressure alarm at the substitution pump segment and blood leaking alarm. The vital signs of the 4 dogs with acute renal failure kept stable during the treatment. After the treatment, a remarkable decrease was seen in the levels of serum urea nitrogen, creatinine and serum potassium (P0.05. Conclusions The mobile blood purification system runs normally even in a field environment. It is a flexible and portable device with a great performance in safety and stability in the treatment of acute renal failure. DOI: 10.11855/j.issn.0577-7402.2013.12.15

  17. Purification of papain by metal affinity partitioning in aqueous two-phase polyethylene glycol/sodium sulfate systems.

    Science.gov (United States)

    Jiang, Zhi-Guo; Zhang, Hai-De; Wang, Wei-Tao

    2015-05-01

    A simple and inexpensive aqueous two-phase affinity partitioning system using metal ligands was introduced to improve the selectivity of commercial papain extraction. Polyethylene glycol 4000 was first activated using epichlorohydrin, then it was covalently linked to iminodiacetic acid. Finally, the specific metal ligand Cu(2+) was attached to the polyethylene glycol-iminodiacetic acid. The chelated Cu(2+) content was measured by atomic absorption spectrometry as 0.88 mol/mol (polyethylene glycol). The effects on the purification at different conditions, including polyethylene glycol molecular weight (2000, 4000, and 6000), concentration of phase-forming components (polyethylene glycol 12-20% w/w and sodium sulfate 12-20%, w/w), metal ligand type, and concentration, system pH and the commercial papain loading on papain extraction, were systematically studied. Under optimum conditions of the system, i.e. 18% w/w sodium sulfate, 18% w/w polyethylene glycol 4000, 1% w/w polyethylene glycol-iminodiacetic acid-Cu(2+) and pH 7, a maximum yield of 90.3% and a degree of purification of 3.6-fold were obtained. Compared to aqueous two phase extraction without ligands, affinity partitioning was found to be an effective technique for the purification of commercial papain with higher extraction efficiency and degree of purification.

  18. [Selection of winter plant species for wetlands constructed as sewage treatment systems and evaluation of their wastewater purification potentials].

    Science.gov (United States)

    Chen, Yong-hua; Wu, Xiao-fu; Chen, Ming-li; Jiang, Li-juan; Li, Ke-lin; Lei, Dian; Wang, Hai-bin

    2010-08-01

    In order to establish an evaluation system for selection of winter wetland plants possessing high wastewater purification potentials in subtropics areas, designed sewage treatment experiments were carried out by introducing into the constructed wetlands 25 species of winter wetland plants. Cluster analysis was performed by including harmful environment-resistant enzyme and substrate enzyme activities into the commonly applied plant screening and assessment indexes system. The obtained results indicated that there were significant differences among the tested winter plants in their root length and vigor, leaf malonaldehyde (MDA), biomass, average nitrogen and phosphorus concentration and uptake, and urease and phosphoric acid enzyme activities in the root areas. Based on the established evaluation system, the tested plants were clustered into 3 groups. The plants in the 1st group possessing high purification potentials are Oenanthe javanica, Brassicacapestris, Juncus effusu, Saxifragaceae, Iris pseudoacorus, Osmanthus fragrans and Iris ensata; those in the 2nd group possessing moderate purification potentials are Brassica oleracea var acephala, Calendula officinalis, Aucuba japonica, Ligustrum lucidu, Beta vulgaris, Rhododendron simsii and Ilex latifolia; and those in the 3rd group with low purification potentials are Brassica oleracea var acephala, Calistephus chinensis, Rosa chinensis, Antirrhinums, Liriope palatyphylla, Zephyranthes candida, Fatshedera lizei, Petunia hybrida, Ilex quihoui, Dianthus caryophyllus and Loropetalum chinensis.

  19. Improving the extraction and purification of immunoglobulin G by the use of ionic liquids as adjuvants in aqueous biphasic systems.

    Science.gov (United States)

    Ferreira, Ana M; Faustino, Vânia F M; Mondal, Dibyendu; Coutinho, João A P; Freire, Mara G

    2016-10-20

    Immunoglobulins G (IgG) could become widespread biopharmaceuticals if cost-efficient processes for their extraction and purification are available. In this work, aqueous biphasic systems (ABS) composed of polyethylene glycols and a buffered salt, and with ionic liquids (ILs) as adjuvants, have been studied as alternative extraction and purification platforms of IgG from a rabbit serum source. Eleven ILs were investigated to provide insights on the chemical features which maximize the IgG partitioning. It is shown that in polymer-salt systems pure IgG preferentially partitions to the polymer-rich phase; yet, the complete extraction was never attained. Remarkably, after the addition of 5wt% of adequate ILs to polymer-salt ABS, the complete extraction of pure IgG in a single-step was accomplished. The best systems and conditions were then applied to the extraction and purification of IgG directly from rabbit serum samples. The complete extraction of IgG in a single-step was maintained while its purity in the polymer-rich phase was enhanced by ca. 37% as compared to the IL-free ABS. The antibody stability was also evaluated revealing that appropriate ILs are able to maintain the IgG stability and can be used as phase-forming components of ABS when envisaging the purification of high-cost biopharmaceuticals.

  20. NO.sub.x catalyst and method of suppressing sulfate formation in an exhaust purification system

    Science.gov (United States)

    Balmer-Millar, Mari Lou; Park, Paul W.; Panov, Alexander G.

    2007-06-26

    The activity and durability of a zeolite lean-burn NOx catalyst can be increased by loading metal cations on the outer surface of the zeolite. However, the metal loadings can also oxidize sulfur dioxide to cause sulfate formation in the exhaust. The present invention is a method of suppressing sulfate formation in an exhaust purification system including a NO.sub.x catalyst. The NO.sub.x catalyst includes a zeolite loaded with at least one metal. The metal is selected from among an alkali metal, an alkaline earth metal, a lanthanide metal, a noble metal, and a transition metal. In order to suppress sulfate formation, at least a portion of the loaded metal is complexed with at least one of sulfate, phosphate, and carbonate.

  1. Rapid purification of quantum systems by measuring in a feedback-controlled unbiased basis

    CERN Document Server

    Combes, Joshua; Jacobs, Kurt; O'Connor, Anthony J

    2010-01-01

    Rapid-purification by feedback --- specifically, reducing the mean impurity faster than by measurement alone --- can be achieved by making the eigenbasis of the density matrix to be unbiased relative to the measurement basis. Here we further examine the protocol introduced by Combes and Jacobs [Phys.~Rev.~Lett.~{\\bf 96}, 010504 (2006)] involving continuous measurement of the observable $J_z$ for a $D$-dimensional system. We rigorously re-derive the lower bound $(2/3)(D+1)$ on the achievable speed-up factor, and also an upper bound, namely $D^2/2$, for all feedback protocols that use measurements in unbiased bases. Finally we extend our results to $n$ independent measurements on a register of $n$ qubits, and derive an upper bound on the achievable speed-up factor that scales linearly with $n$.

  2. Study on Purification Diatomite with nitric acid by Thermal Closed System

    Directory of Open Access Journals (Sweden)

    Kuang Meng

    2016-01-01

    Full Text Available In this research, a purification approach using nitric acid leaching at thermal closed system was developed to improve the porous structure of raw diatomite by removal of impurities from its surface and clogged pores. The feasibility and efficiency of this approach were determined by XRF for chemical constitution of diatomite, SEM for morphology and BET for specific surface area of purified diatomite. The investigations indicated that the content of SiO2 was in order of 85.14% for raw diatomite and 98% for purified diatomite, the content of Fe2O3 decreases after purified; the integrity of the porous structure was confirmed by SEM, and increase in specific surface area from 18m2·g-1 to 36m2·g-1.

  3. Single-Step Purification of Ovalbumin from Egg White Using Aqueous Biphasic Systems.

    Science.gov (United States)

    Pereira, Matheus M; Cruz, Rafaela A P; Almeida, Mafalda R; Lima, Álvaro S; Coutinho, João A P; Freire, Mara G

    2016-06-01

    The ability of aqueous biphasic systems (ABS) composed of polyethylene glycols of different molecular weights (PEG 400, 600 and 1000) and buffered aqueous solutions of potassium citrate/citric acid (pH = 5.0 - 8.0) to selectively extract ovalbumin from egg white was here investigated. Phase diagrams, tie-lines and tie-line lengths were determined at 25ºC and the partitioning of ovalbumin in these systems was then evaluated. Aiming at optimizing the selective extraction of ovalbumin in the studied ABS, factors such as pH, PEG molecular weight and amount of the phase-forming components were initially investigated with pure commercial ovalbumin. In almost all ABS, it was observed a preferential partitioning of ovalbumin to the polymer-rich phase, with extraction efficiencies higher than 90%. The best ABS were then applied in the purification of ovalbumin from the real egg white matrix. In order to ascertain on the ovalbumin purity and yield, sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and size exclusion high performance liquid chromatography (SE-HPLC) analyses were conducted, confirming that the isolation/purification of ovalbumin from egg white was completely achieved in a single-step with a recovery yield of 65%. The results obtained show that polymer-salt-based ABS allow the selective extraction of ovalbumin from egg white with a simpler approach and better performance than previously reported. Finally, it is shown that ovalbumin can be completely recovered from the PEG-rich phase by an induced precipitation using an inexpensive and sustainable separation platform which can be easily applied on an industrial scale.

  4. Design of functional guanidinium ionic liquid aqueous two-phase systems for the efficient purification of protein

    Energy Technology Data Exchange (ETDEWEB)

    Ding, Xueqin; Wang, Yuzhi, E-mail: wyzss@hnu.edu.cn; Zeng, Qun; Chen, Jing; Huang, Yanhua; Xu, Kaijia

    2014-03-01

    Graphical abstract: - Highlights: • A series of novel cationic functional hexaalkylguanidinium ionic liquids and anionic functional tetraalkylguanidinium ionic liquids have been synthesized. • Functional guanidinium ionic liquid aqueous two-phase systems have been first designed for the purification of protein. • Mechanisms and performances of the process were researched. • Simple, green, safety and presents better purified ability than ordinary process. • A potential efficient platform for protein purification and related studies. - Abstract: A series of novel cationic functional hexaalkylguanidinium ionic liquids and anionic functional tetraalkylguanidinium ionic liquids have been devised and synthesized based on 1,1,3,3-tetramethylguanidine. The structures of the ionic liquids (ILs) were confirmed by {sup 1}H nuclear magnetic resonance ({sup 1}H NMR) and 13C nuclear magnetic resonance (13C NMR) and the production yields were all above 90%. Functional guanidinium ionic liquid aqueous two-phase systems (FGIL-ATPSs) have been first designed with these functional guanidinium ILs and phosphate solution for the purification of protein. After phase separation, proteins had transferred into the IL-rich phase and the concentrations of proteins were determined by measuring the absorbance at 278 nm using an ultra violet visible (UV–vis) spectrophotometer. The advantages of FGIL-ATPSs were compared with ordinary ionic liquid aqueous two-phase systems (IL-ATPSs). The proposed FGIL-ATPS has been applied to purify lysozyme, trypsin, ovalbumin and bovine serum albumin. Single factor experiments were used to research the effects of the process, such as the amount of ionic liquid (IL), the concentration of salt solution, temperature and the amount of protein. The purification efficiency reaches to 97.05%. The secondary structure of protein during the experimental process was observed upon investigation using UV–vis spectrophotometer, Fourier-transform infrared

  5. Efeito da bomba de infusão de soluções sobre o grau de hemólise em concentrados de hemácias Effect of the infusion pumps of solutions on the degree of hemolysis

    Directory of Open Access Journals (Sweden)

    Eunice B. Carvalho

    2007-06-01

    Full Text Available A lise das hemácias, de causa imune ou não imune, pode provocar a elevação da hemoglobina plasmática induzindo efeitos deletérios principalmente nos rins e no sistema cardiovascular. Este trabalho objetiva evidenciar o possível efeito hemolítico induzido pelo trauma mecânico provocado pela passagem das hemácias em diferentes modelos de bombas de infusão de soluções, em concentrados de hemácias com até dez dias de armazenamento, preparados com Sag-Manitol. Foram utilizados três modelos de bombas (Nutrimat, Infusomat Compact e Volumed, com quatro aparelhos de cada modelo, testados em três velocidades de infusão (120 mL/h, 240 mL/h e 360 mL/h. Os parâmetros utilizados para evidenciar o grau de hemólise foram o percentual de hemólise e os níveis de hemoglobina e potássio livres no plasma. As amostras foram coletadas antes da passagem do concentrado de hemácias pelas bombas, na metade do tempo de infusão e no final da infusão, nas diferentes velocidades. Não ocorreu variação significativa entre os valores dos parâmetros analisados nas amostras controle e naquelas coletadas nas diferentes velocidades, marcas de bombas, mecanismos de infusão entre as bombas da mesma marca e entre os diferentes tempos de infusão. A principal variação encontrada foi relacionada com o potássio livre, devido à variação inerente aos próprios concentrados, o que foi evidenciado ao se compararem os valores obtidos nas amostras controle e nas amostras colhidas nos tempos médio e final em cada bomba. De acordo com os resultados obtidos, não houve alteração estatisticamente significativa do percentual de hemólise após a passagem pelas bombas de infusão nos modelos analisados.The lyses of red blood cells, both for immune or non-immune reasons, can cause an elevation in plasmatic hemoglobin, inducing harmful effects mainly in the kidneys and cardiovascular system. This work aims at identifying the possible hemolytic effect, related to

  6. Chemical resistance of the gram-negative bacteria to different sanitizers in a water purification system

    Directory of Open Access Journals (Sweden)

    Penna Thereza CV

    2006-08-01

    Full Text Available Abstract Background Purified water for pharmaceutical purposes must be free of microbial contamination and pyrogens. Even with the additional sanitary and disinfecting treatments applied to the system (sequential operational stages, Pseudomonas aeruginosa, Pseudomonas fluorescens, Pseudomonas alcaligenes, Pseudomonas picketti, Flavobacterium aureum, Acinetobacter lowffi and Pseudomonas diminuta were isolated and identified from a thirteen-stage purification system. To evaluate the efficacy of the chemical agents used in the disinfecting process along with those used to adjust chemical characteristics of the system, over the identified bacteria, the kinetic parameter of killing time (D-value necessary to inactivate 90% of the initial bioburden (decimal reduction time was experimentally determined. Methods Pseudomonas aeruginosa, Pseudomonas fluorescens, Pseudomonas alcaligenes, Pseudomonas picketti, Flavobacterium aureum, Acinetobacter lowffi and Pseudomonas diminuta were called in house (wild bacteria. Pseudomonas diminuta ATCC 11568, Pseudomonas alcaligenes INCQS , Pseudomonas aeruginosa ATCC 15442, Pseudomonas fluorescens ATCC 3178, Pseudomonas picketti ATCC 5031, Bacillus subtilis ATCC 937 and Escherichia coli ATCC 25922 were used as 'standard' bacteria to evaluate resistance at 25°C against either 0.5% citric acid, 0.5% hydrochloric acid, 70% ethanol, 0.5% sodium bisulfite, 0.4% sodium hydroxide, 0.5% sodium hypochlorite, or a mixture of 2.2% hydrogen peroxide (H2O2 and 0.45% peracetic acid. Results The efficacy of the sanitizers varied with concentration and contact time to reduce decimal logarithmic (log10 population (n cycles. To kill 90% of the initial population (or one log10 cycle, the necessary time (D-value was for P. aeruginosa into: (i 0.5% citric acid, D = 3.8 min; (ii 0.5% hydrochloric acid, D = 6.9 min; (iii 70% ethanol, D = 9.7 min; (iv 0.5% sodium bisulfite, D = 5.3 min; (v 0.4% sodium hydroxide, D = 14.2 min; (vi 0.5% sodium

  7. Design of functional guanidinium ionic liquid aqueous two-phase systems for the efficient purification of protein.

    Science.gov (United States)

    Ding, Xueqin; Wang, Yuzhi; Zeng, Qun; Chen, Jing; Huang, Yanhua; Xu, Kaijia

    2014-03-07

    A series of novel cationic functional hexaalkylguanidinium ionic liquids and anionic functional tetraalkylguanidinium ionic liquids have been devised and synthesized based on 1,1,3,3-tetramethylguanidine. The structures of the ionic liquids (ILs) were confirmed by (1)H nuclear magnetic resonance ((1)H NMR) and 13C nuclear magnetic resonance (13C NMR) and the production yields were all above 90%. Functional guanidinium ionic liquid aqueous two-phase systems (FGIL-ATPSs) have been first designed with these functional guanidinium ILs and phosphate solution for the purification of protein. After phase separation, proteins had transferred into the IL-rich phase and the concentrations of proteins were determined by measuring the absorbance at 278 nm using an ultra violet visible (UV-vis) spectrophotometer. The advantages of FGIL-ATPSs were compared with ordinary ionic liquid aqueous two-phase systems (IL-ATPSs). The proposed FGIL-ATPS has been applied to purify lysozyme, trypsin, ovalbumin and bovine serum albumin. Single factor experiments were used to research the effects of the process, such as the amount of ionic liquid (IL), the concentration of salt solution, temperature and the amount of protein. The purification efficiency reaches to 97.05%. The secondary structure of protein during the experimental process was observed upon investigation using UV-vis spectrophotometer, Fourier-transform infrared spectroscopy (FT-IR) and circular dichroism spectrum (CD spectrum). The precision, stability and repeatability of the process were investigated. The mechanisms of purification were researched by dynamic light scattering (DLS), determination of the conductivity and transmission electron microscopy (TEM). It was suggested that aggregation and embrace phenomenon play a significant role in the purification of proteins. All the results show that FGIL-ATPSs have huge potential to offer new possibility in the purification of proteins.

  8. Improvement of xenon purification system using a combination of a pulse tube refrigerator and a coaxial heat exchanger

    CERN Document Server

    Chen, Wan-Ting; Cussonneau, J -P; Donnard, J; Duval, S; Lemaire, O; Calloch, M Le; Ray, P Le; Mohamad-Hadi, A -F; Morteau, E; Oger, T; Scotto-Lavina, L; Stutzmann, J -S; Thers, D; Briend, P; Haruyama, T; Mihara, S; Tauchi, T

    2012-01-01

    We have developed a compact cryogenic system with a pulse tube refrigerator and a coaxial heat exchanger. This liquefaction-purification system not only saves the cooling power used to reach high gaseous recirculation rate, but also reduces the impurity level with high speed. The heat exchanger operates with an efficiency of 99%, which indicates the possibility for fast xenon gas recirculation in a highpressurized large-scale xenon storage with much less thermal losses.

  9. The study of recirculating aquaculture system in pond and its purification effect

    Science.gov (United States)

    Qu, Jiangqi; Zhang, Qingjing; Jia, Chengxia; Liu, Pan; Yang, Mu

    2017-05-01

    In this paper, a recirculating aquaculture purification system (RAPS) was designed to solve the problems of aquaculture pollution and shortage of freshwater resource according to the characteristic of northern freshwater ponds of China. The system were arranged in series and composed of high density culture pond, deposit pond, floating and submerged plant pond, ecological floating bed pond and biofilm filtrate pond. At the fish density of 20~30kg/m3 in the high density culture pond, the water quality parameters were monitored seasonally. The results indicated that the removal rate of total nitrogen, total phosphorus, ammonia nitrogen and nitrite nitrogen in the recirculating aquaculture system were 69.59%, 77.89%, 72.54% and 68.68%, respectively. The floating and submerged plant pond and ecological floating bed pond can remove TN and TP obviously, and increase dissolved oxygen and transparency significantly. And the biofilm filtrate pond has good effect of removing ammonium nitrogen and nitrite nitrogen, meanwhile, the microbial communities in the recirculating aquaculture system regulate on the water quality. Therefore, the RAPS show significant effects on water saving and pollution emission reducing.

  10. Expression and Purification of ZNF191(243-368) in Three Expression Systems

    Institute of Scientific and Technical Information of China (English)

    ZHAO Dong-Xin; TENG Xin-Cheng; DING Zhi-Chun; HUANG Zhong-Xian

    2007-01-01

    ZNF191(243-368), a new human zinc finger protein, probably relates to some hereditary diseases and cancers.To obtain adequate amount of ZNF 191 (243-368) for the study of its property, structure and function, three different expression systems of inclusion-body, glutathione S-transferase (GST), and hexahistidine (6 × His) were used and compared. Among these systems, the expression level of ZNF191(243-368) was increased in inclusion body system under a higher isopropylthio-β-D-galactoside (IPTG) concentration, but the non-target proteins were also increased more, which made its purification more difficult and the yield lower. The expression of His-tag fusion protein was almost not affected by IPTG concentration, temperature and inducing time. At a high IPTG concentration the highest expression yield for GST fusion protein was obtained. And the fusion proteins can be partially purified by a single affinity chromatography step. The fusion protein systems show advantages for expression of these proteins.

  11. Evaluation of systems for purification of fuel gas from anaerobic digestion. Engineering report

    Energy Technology Data Exchange (ETDEWEB)

    Ashare, E.; Augenstein, D. C.; Yeung, J. C.; Hossan, R. J.; Duret, G. L.

    1978-07-30

    Fuel gas obtained from the anaerobic digestion of waste materials usually needs to be treated before being transmitted in existing pipeline systems. The purification scheme involves the removal of carbon dioxide, hydrogen sulfide, and moisture from the digester gas to meet pipeline specifications. Gas treatment systems for the handling of 0.1, 1.0, and 3.0 MM Scfd of raw feed gas and product delivery pressures of 125 and 1000 psia were considered in this study. From the results of an economic and technical analysis of these systems, physical absorption systems, particularly water scrubbing, were found to be the most economical system for the treatment of digester gas with the flow streams considered. The Fluor Solvent process was economically comparable with the water scrubbing process for high pressure applications, but the value presented had a large uncertainty. The commercial chemical absorption systems were less economical due to high heat requirements for solvent regeneration. Among the chemical absorption processes, the hot potassium systems were found to be more economical than the amine system. The molecular sieve adsorption process was economically similar to the chemical absorption process. The GE membrane separation process was economically comparable for both high and low pressure apllications, but this system has not been evaluated in real-life conditions. Since no kinetic and equilibrium data were available for the phosphate buffer system, estimates had to be assumed. Using what were believed to be very conservative estimates, this chemical absorption process was found to be economically competitive with the water scrubbing process for treating gas with a delivery pressure of 125 psia.The cost analyses in this study were based on the assumption of no recovery of the CO/sub 2/ by-product. It was found that credits for the recovery of the CO/sub 2/ could be significant.

  12. Determinación de los parámetros de funcionamiento de bombas y motores hidráulicos

    OpenAIRE

    Luciano Pérez Sobrevilla; Pedro Paneque Rondón; Héctor de las Cuevas Milán

    2009-01-01

    Se presenta el manual de usuario del software Performance Curves para la determinación teórica y rápida de los parámetros de funcionamiento de las máquinas hidráulicas (bombas y motores), evitando la tediosa y lenta evaluación experimental e incrementando la eficiencia de la actividad con la menor cantidad de personal. El software posibilita determinar los parámetros adecuados de los regímenes óptimos de trabajo de la máquina hidráulica, las relaciones entre la eficiencia de la bomba y del ...

  13. As bombas atômicas podem dizimar a humanidade - Hiroshima e Nagasaki, há 70 anos

    Directory of Open Access Journals (Sweden)

    OKUNO EMICO

    2015-08-01

    Full Text Available Este artigo rememora o que aconteceu há 70 anos após o lançamento pelos americanos das bombas atômicas Little Boy e Fat Man, respectivamente, nas cidades japonesas de Hiroshima e Nagasaki. Discorre suscintamente sobre energia nuclear e o Projeto Manhattan, que desenvolveu e produziu essas bombas e suas características. A destruição causada e os efeitos biológicos consequentes na população são também descritos.

  14. Purification of chlorogenic acid in Flos Lonicerae with system of polar ordered resins

    Institute of Scientific and Technical Information of China (English)

    XIANG Zhi-nan; ZHAN Yu; NING Zheng-xiang

    2007-01-01

    A system of polar ordered resins was established for purification of chlorogenic acid in Flos Lonicerae. It was composed of three reversed phase resins, AB-8, DM-130 and NKA-9, representative for their gradually increased polarity and selectivity. A method of RP-HPLC was used for determination of chlorogenic acid. And the performance of adsorption and desorption for chlorogenic acid with the system of polar ordered resins was studied. Furthermore, the effects of concentration, pH and flow rate of the adsorbate on adsorption ability were researched. It is indicated that the optimum parameters for chlorogenic acid are as follows:pH 3.5 with a flow rate of 2.5 BV/h, the concentration of extract solution at 0.50, 0.40, 0.30 g/L respectively for the adsorptive operation twice, and 6.93, 8.66, 10.39 mol/L ethanol used as gradient eluants. The purity of resulted product of chlorogenic acid arrives 70.20% with yield of 89.79%. With simple procedures, low costs and high purity product, the method of system of polar ordered resins followed by sequential reversed phase separations can be used to refine the chlorogenic acid in the extraction of Flos Lonicerae.

  15. EVALUACIÓN ERGONÓMICA DEL USO DE BOMBAS PORTATILES PARA FUMIGAR

    OpenAIRE

    2007-01-01

    Como primera actividad se observó al operario trabajando, anotando cualquier detalle que fuera relevante para la investigación, posteriormente se evaluó con el método “e-RULA” el uso de las mochilas para fumigar cuando se encuentran con el liquido para fumigar y cuando se encuentran vacías. Adicionalmente se aplicaron encuestas a 30 empleados campesinos (hombres) que laboran en ranchos y tienen por actividad cotidiana el fumigar con bombas portátiles (tipo mochila) los sembradíos. En el ranch...

  16. Contribuição ao projeto de bomba centrifuga de sangue

    OpenAIRE

    Gastão Dias Motta

    1991-01-01

    Resumo: Este trabalho tem como objetivo basico contribuir para o desenvolvimento de uma bomba centrifuga de sangue funcionando sob o principio do arrasto viscoso entre uma ou mais superficies lisas rotativas e o sangue. A sua elaboração pode ser dividida numa parte experimental e numa parte teorica, as quais foram desenvolvidas de maneira simultanea mas absolutamente independentes entre...Observação: O resumo, na íntegra poderá ser visualizado no texto completo da tese digital Abstract: Th...

  17. Purification and assays of Rhodobacter capsulatus RegB-RegA two-component signal transduction system.

    Science.gov (United States)

    Swem, Lee R; Swem, Danielle L; Wu, Jiang; Bauer, Carl E

    2007-01-01

    Two-component signal-transduction systems, composed of a histidine-sensor kinase and a DNA-binding response regulator, allow bacteria to detect environmental changes and adjust cellular physiology to live more efficiently in a broad distribution of niches. Although many two-component signal-transduction systems are known, a limited number of signals that stimulate these systems have been discovered. This chapter describes the purification and characterization of the predominant two-component signal-transduction system utilized by Rhodobacter capsulatus, a nonsulfur purple photosynthetic bacterium. Specifically, we explain the overexpression, detergent solubilization, and purification of the full-length membrane-spanning histidine-sensor kinase RegB. We also provide a method to measure autophosphorylation of RegB and discern the effect of its signal molecule, ubiquinone, on autophosphorylation levels. In addition we describe the overexpression and purification of the cognate response regulator RegA and a technique used to visualize the phosphotransfer reaction from RegB to RegA.

  18. Simulation of a hydrogen production and purification system for a PEM fuel-cell using bioethanol as raw material

    Energy Technology Data Exchange (ETDEWEB)

    Giunta, Pablo; Amadeo, Norma; Laborde, Miguel [Facultad de Ingenieria, Universidad de Buenos Aires, Laboratorio de Procesos Cataliticos, Pabellon de Industrias, Ciudad Universitaria, 1428 Buenos Aires (Argentina); Mosquera, Carlos [Facultad de Ingenieria, Universidad de Buenos Aires, Departamento de Fisica, 1063 Buenos Aires (Argentina)

    2007-01-10

    A process to produce 'fuel-cell grade' hydrogen from ethanol steam reforming is analyzed from a thermodynamic point of view. The hydrogen purification process consists of WGS and COPROX reactors. Equations to evaluate the efficiency of the system, including the fuel cell, are presented. A heat exchange network is proposed in order to improve the exploitation of the available power. The effect of key variables such as the reformer temperature and the ethanol/water molar feed ratio on the fuel-cell efficiency is discussed. Results show that it is feasible to carry out the energy integration of the hydrogen catalytic production and purification-PEM fuel-cell system, using ethanol as raw material. The technology of 'fuel-cell grade' hydrogen production using ethanol as raw material is a very attractive alternative to those technologies based in fossil fuels. (author)

  19. Fractionated plasma separation and adsorption system: a novel system for blood purification to remove albumin bound substances.

    Science.gov (United States)

    Falkenhagen, D; Strobl, W; Vogt, G; Schrefl, A; Linsberger, I; Gerner, F J; Schoenhofen, M

    1999-01-01

    The removal of albumin bound substances has gained increasing interest in different diseases, especially in acute and chronic liver disease. Therefore, a new system, the fractionated plasma separation and adsorption (FPSA) system, was developed based on combined membrane and adsorbent blood purification techniques. The most important contribution to the FPSA system was the development of a new polysulfone hollow-fiber filter, which is characterized by a sieving coefficient of 0.89 for human serum albumin (HSA) but only of 0.17 for fibrinogen, and 0 (zero) for IgM immunoglobulins. Using a closed filtrate circuit connected to the new polysulfone filter which integrates 1 or 2 adsorption columns and also a high flux dialyzer adapted to a dialysis machine, the FPSA system opens excellent possibilities for the relatively specific removal of albumin bound substances from the blood such as albumin bound bilirubin or even tryptophan. In comparison to other systems (for example, the Molecular Adsorbent Recirculating System [MARS] and albumin dialysis systems), the FPSA system enables much higher elimination of strongly bound albumin substances. The first clinical investigations have recently started based on a modified dialysis machine designed with all necessary safety measures.

  20. Submersible pumps: Energetic efficiency norm; Bombas sumergibles: norma de eficiencia energetica

    Energy Technology Data Exchange (ETDEWEB)

    Acosta Torres, R. A.; Buendia Dominguez, E. H. [Instituto de Investigaciones Electricas, Cuernavaca (Mexico)

    1997-12-31

    The Instituto de Investigaciones Electricas (IIE), supporting the Comision Nacional para el Ahorro de Energia (CONAE), has prepared a preliminary project of the norm of energy efficiency for submersible pumps. This document has complied with the necessary formalities for the emission of the norm. This paper presents the results of the analysis of the submersible pumps, the energy savings, as well as the economic evaluation that sustains the norm project, additionally pointing out the environmental impact that will bring along the implantation of the same. [Espanol] El Instituto de Investigaciones Electricas (IIE), apoyando a la Comision Nacional para el Ahorro de Energia (CONAE), ha elaborado el anteproyecto de norma de eficiencia energetica para bombas sumergibles. Dicho documento ha cumplido con los tramites necesarios para la emision de una norma. En el presente articulo se mencionan los resultados del analisis de las bombas sumergibles, los ahorros energeticos, asi como la evaluacion economica que sustenta el proyecto de norma, indicandose ademas el impacto ambiental que tendra la implantacion de la misma.

  1. AM-DMC-AMPS Multi-Functionalized Magnetic Nanoparticles for Efficient Purification of Complex Multiphase Water System

    Science.gov (United States)

    Ge, Yuru; Li, Yushu; Zu, Baiyi; Zhou, Chaoyu; Dou, Xincun

    2016-04-01

    Complex multiphase waste system purification, as one of the major challenges in many industrial fields, urgently needs an efficient one-step purification method to remove several pollutants simultaneously and efficiently. Multi-functionalized magnetic nanoparticles, Fe3O4@SiO2-MPS-AM-DMC-AMPS, were facilely prepared via a one-pot in situ polymerization of three different functional monomers, AM, DMC, and AMPS, on a Fe3O4@SiO2-MPS core-shell structure. The multi-functionalized magnetic nanoparticles (MNPs) are proven to be a highly effective purification agent for oilfield wastewater, an ideal example of industrial complex multiphase waste system containing cations, anions, and organic pollutants. Excellent overall removal efficiencies for both cations, including K+, Ca2+, Na+, and Mg2+ of 80.68 %, and anions, namely Cl- and SO4 2-, of 85.18 % along with oil of 97.4 % were shown. The high removal efficiencies are attributed to the effective binding of the functional groups from the selected monomers with cations, anions, and oil emulsions.

  2. AM-DMC-AMPS Multi-Functionalized Magnetic Nanoparticles for Efficient Purification of Complex Multiphase Water System.

    Science.gov (United States)

    Ge, Yuru; Li, Yushu; Zu, Baiyi; Zhou, Chaoyu; Dou, Xincun

    2016-12-01

    Complex multiphase waste system purification, as one of the major challenges in many industrial fields, urgently needs an efficient one-step purification method to remove several pollutants simultaneously and efficiently. Multi-functionalized magnetic nanoparticles, Fe3O4@SiO2-MPS-AM-DMC-AMPS, were facilely prepared via a one-pot in situ polymerization of three different functional monomers, AM, DMC, and AMPS, on a Fe3O4@SiO2-MPS core-shell structure. The multi-functionalized magnetic nanoparticles (MNPs) are proven to be a highly effective purification agent for oilfield wastewater, an ideal example of industrial complex multiphase waste system containing cations, anions, and organic pollutants. Excellent overall removal efficiencies for both cations, including K(+), Ca(2+), Na(+), and Mg(2+) of 80.68 %, and anions, namely Cl(-) and SO4 (2-), of 85.18 % along with oil of 97.4 % were shown. The high removal efficiencies are attributed to the effective binding of the functional groups from the selected monomers with cations, anions, and oil emulsions.

  3. Extraction and purification of wheat-esterase using aqueous two-phase systems of ionic liquid and salt.

    Science.gov (United States)

    Jiang, Bin; Feng, Zhibiao; Liu, Chunhong; Xu, Yingcao; Li, Dongmei; Ji, Guo

    2015-05-01

    To explore a new and simple rapid extraction and purification technique for wheat-esterase, an ionic liquids (ILs)-based aqueous two-phase system (ATPS) was developed for the purification of wheat-esterase from wheat extracts. Effects of various process parameters such as the concentrations of [Bmim]BF4, the types and concentrations of phase-forming salt, the system pH and the temperature on partitioning of wheat-esterase were evaluated. The obtained data indicated that wheat-esterase was preferentially partitioned into the ILs-rich phase and the ATPS composed of 20 % [Bmim]BF4 (w/w) and 25 % (w/w) NaH2PO4(pH = 4.8) showed good selectivity on wheat-esterase. Under the optimum conditions, wheat-esterase was purified with an acceptable yield (88.93 %), but produced wheat-esterase was 4.23 times as pure. It was obvious that temperature shows little influence on the purification between 10 and 50 °C. Sephadex G-150FF revealed that the band intensity of contaminating proteins in ATPS fraction almost disappeared. Therefore, ILs-based ATPS was an effective method for partitioning and recovery of wheat-esterase from wheat crude extracts.

  4. DOE Hydrogen, Fuel Cells and Infrastructure Technologies Program Integrated Hydrogen Production, Purification and Compression System

    Energy Technology Data Exchange (ETDEWEB)

    Tamhankar, Satish; Gulamhusein, Ali; Boyd, Tony; DaCosta, David; Golben, Mark

    2011-06-30

    The project was started in April 2005 with the objective to meet the DOE target of delivered hydrogen of <$1.50/gge, which was later revised by DOE to $2-$3/gge range for hydrogen to be competitive with gasoline as a fuel for vehicles. For small, on-site hydrogen plants being evaluated at the time for refueling stations (the 'forecourt'), it was determined that capital cost is the main contributor to the high cost of delivered hydrogen. The concept of this project was to reduce the cost by combining unit operations for the entire generation, purification, and compression system (refer to Figure 1). To accomplish this, the Fluid Bed Membrane Reactor (FBMR) developed by MRT was used. The FBMR has hydrogen selective, palladium-alloy membrane modules immersed in the reformer vessel, thereby directly producing high purity hydrogen in a single step. The continuous removal of pure hydrogen from the reformer pushes the equilibrium 'forward', thereby maximizing the productivity with an associated reduction in the cost of product hydrogen. Additional gains were envisaged by the integration of the novel Metal Hydride Hydrogen Compressor (MHC) developed by Ergenics, which compresses hydrogen from 0.5 bar (7 psia) to 350 bar (5,076 psia) or higher in a single unit using thermal energy. Excess energy from the reformer provides up to 25% of the power used for driving the hydride compressor so that system integration improved efficiency. Hydrogen from the membrane reformer is of very high, fuel cell vehicle (FCV) quality (purity over 99.99%), eliminating the need for a separate purification step. The hydride compressor maintains hydrogen purity because it does not have dynamic seals or lubricating oil. The project team set out to integrate the membrane reformer developed by MRT and the hydride compression system developed by Ergenics in a single package. This was expected to result in lower cost and higher efficiency compared to conventional hydrogen production

  5. Development of Android app for designing spur gear pumps = Desarrollo de aplicación Android para el diseño de bombas de engranajes

    OpenAIRE

    González Demianenko, Manuel

    2015-01-01

    La tecnología de las bombas de engranajes tiene un potencial enorme en el mundo industrial, desde la alimentación en motores diesel de nueva generación hasta la dosificación de fluidos en sistemas médicos y farmacéuticos. El principal componente de dichas bombas son sus engranajes, y su cálculo y diseño debe tener en cuenta todas las variables posibles a la hora de trabajar como parte de una bomba. El conocimiento actual sobre ellos, así como sus criterios de diseño y otros parámetros, pueden...

  6. A generic system for the expression and purification of soluble and stable influenza neuraminidase.

    Directory of Open Access Journals (Sweden)

    Peter M Schmidt

    Full Text Available The influenza surface glycoprotein neuraminidase (NA is essential for the efficient spread of the virus. Antiviral drugs such as Tamiflu (oseltamivir and Relenza (zanamivir that inhibit NA enzyme activity have been shown to be effective in the treatment of influenza infections. The recent 'swine flu' pandemic and world-wide emergence of Tamiflu-resistant seasonal human influenza A(H1N1 H(274Y have highlighted the need for the ongoing development of new anti-virals, efficient production of vaccine proteins and novel diagnostic tools. Each of these goals could benefit from the production of large quantities of highly pure and stable NA. This publication describes a generic expression system for NAs in a baculovirus Expression Vector System (BEVS that is capable of expressing milligram amounts of recombinant NA. To construct NAs with increased stability, the natural influenza NA stalk was replaced by two different artificial tetramerization domains that drive the formation of catalytically active NA homotetramers: GCN4-pLI from yeast or the Tetrabrachion tetramerization domain from Staphylothermus marinus. Both recombinant NAs are secreted as FLAG-tagged proteins to allow for rapid and simple purification. The Tetrabrachion-based NA showed good solubility, increased stability and biochemical properties closer to the original viral NA than the GCN4-pLI based construct. The expressed quantities and high quality of the purified recombinant NA suggest that this expression system is capable of producing recombinant NA for a broad range of applications including high-throughput drug screening, protein crystallisation, or vaccine development.

  7. Purification Performance and Production of a Re-circulating Pond Aquaculture System Based on Paddy Field

    Directory of Open Access Journals (Sweden)

    Gu Li

    2012-10-01

    Full Text Available Developing improved aquaculture systems with a more efficient use of water and less environmental impact is becoming a crying need. A re-circulating aquaculture system consisting of paddy field and fish pond is a new culture mode due to aquaculture combing with agriculture. The present study focused on the purification capacity of the paddy field on nitrogen, phosphorus and organic matter, the fluctuation trend of water quality conditions during the whole rearing process and the culture efficacy of the main culture species of grass carp (Ctenopharyngodon idella. The results were as follows: under a flow rate of 1.4-5.5 m3/h for the recirculation treatment, the average removal rate of ammonia nitrogen, nitrate nitrogen, total nitrogen, total phosphorus and biochemical oxygen demand for the aquaculture effluent amounted to 40.5, 43.5, 31.9, 23.9, 20.7 and 52.4%, respectively, But the dissolved oxygen content in the rice fields increased obviously. During the whole process of fish rearing, the main physicochemical parameters of water quality for the experimental ponds were all maintained at a suitable level for the growth of the grass carp. In addition, there were significant differences (p<0.05 in DO, TSS, NH4+ -N, NO--N, BOD5 and Chl-&alpha between the experimental and control ponds. As far as the yield per unit and survival rate was concerned, the level of the experimental ponds was obviously higher than that of the control, while the feed conversion ratio displayed the opposite trend. Overall, the new aquaculture system realized the double aims of water reuse and the reduction of waste water discharge.

  8. Diseño y construcción de un control de temperatura para el fluido del banco de pruebas para bombas hidráulicas de desplazamiento positivo

    OpenAIRE

    Monroy J., Mauricio; García, Christian Julián; Marquéz V., José Alirio

    2009-01-01

    El presente trabajo describe el diseño y construcción de un sistema de control de temperatura del fluido de trabajo para el banco de pruebas para bombas hidráulicas de desplazamiento positivo, presente en el laboratorio de sistemas dinámicos, utilizando un sistema de enfriamiento, comandado por microcontrolador, con comunicación a computador. The present work describes the design and construction of a working fluid temperature control system for the test bench for positive displ...

  9. Comparison of the role of gibberellins and ethylene in response to submergence of two lowland rice cultivars, Senia and Bomba.

    Science.gov (United States)

    Dubois, Vincent; Moritz, Thomas; García-Martínez, José L

    2011-02-15

    We examined the gibberellin (GA) and ethylene regulation of submergence-induced elongation in seedlings of the submergence-tolerant lowland rice (Oryza sativa L.) cvs Senia and Bomba. Elongation was enhanced after germination to facilitate water escape and reach air. We found that submergence-induced elongation depends on GA because it was counteracted by paclobutrazol (an inhibitor of GA biosynthesis), an effect that was negated by GA(3). Moreover, in the cv Senia, submergence increased the content of active GA(1) and its immediate precursors (GA(53), GA(19) and GA(20)) by enhancing expression of several GA biosynthesis genes (OsGA20ox1 and -2, and OsGA3ox2), but not by decreasing expression of several OsGA2ox (GA inactivating genes). Senia seedlings, in contrast to Bomba seedlings, did not elongate in response to ethylene or 1-aminocyclopropane-1-carboxylic-acid (ACC; an ethylene precursor) application, and submergence-induced elongation was not reduced in the presence of 1-methylcyclopropene (1-MCP; an ethylene perception inhibitor). Ethylene emanation was similar in Senia seedlings grown in air and in submerged-grown seedlings following de-submergence, while it increased in Bomba. The expression of ethylene biosynthesis genes (OsACS1, -2 and -3, and OsACO1) was not affected in Senia, but expression of OsACS5 was rapidly enhanced in Bomba upon submergence. Our results support the conclusion that submergence elongation enhancement of lowland rice is due to alteration of GA metabolism leading to an increase in active GA (GA(1)) content. Interestingly, in the cv Senia, in contrast to cv Bomba, this was triggered through an ethylene-independent mechanism.

  10. Development of a tool for the analysis and diagnosis in real time of centrifugal pumps; Desarrollo en una herramienta para el analisis y diagnostico en tiempo real de bombas centrifugas

    Energy Technology Data Exchange (ETDEWEB)

    Pacheco, J.J.; Aviles, J.J.; Zaleta, A.; Olivares, A. [Facultad de Ingenieria Mecanica, Electrica y Electronica (FIMEE), Universidad de Guanajuato (Mexico)

    2007-11-15

    In this paper the development of a computer tool for the analysis of centrifugal pumps is presented. This tool allows the user to analyze the performance of the pump by means of the analysis of the behavior curves at its design conditions, reference and operation. In order to realize the analysis it is necessary that the user feeds the tool with the missing and necessary information according to norm ASME PTC 8.2 and under the specified conditions of calibration of the same norm, to eliminate possible errors in the results, a bad qualification or erroneous acquisition of the signals. The system must early be fed with the polynomials of the behavior curves of the pump in its design conditions to later correct the behavior based on a velocity of present operation. [Spanish] En este trabajo se presenta el desarrollo de una herramienta computacional para el analisis de bombas centrifugas. Dicha herramienta permite al usuario analizar el desempeno de la bomba mediante el analisis de las curvas de comportamiento en sus condiciones de diseno, referencia y operacion. Para realizar el analisis es necesario que el usuario alimente la herramienta con la informacion faltante y necesaria de acuerdo a la norma ASME PTC 8.2 y bajo las condiciones de calibracion especificadas en la misma norma, para eliminar posibles errores en los resultados a una mala calificacion o adquisicion erronea de las senales. El sistema tiene que ser anticipadamente alimentado con los polinomios de las curvas de comportamiento de la bomba en sus condiciones de diseno para posteriormente corregir el comportamiento en funcion de una velocidad de operacion actual.

  11. Detección de cavitación en una bomba centrífuga usando emisiones acústicas Cavitation detection in a centrifugal pump using acoustic emissions

    Directory of Open Access Journals (Sweden)

    Jabid Quiroga M.

    2012-12-01

    Full Text Available En el presente artículo se propone el uso de las emisiones acústicas para el monitoreo de la cavitación en una bomba centrífuga. Este monitoreo se ejecuta a través del seguimiento a unos indicadores de falla obtenidos a partir del valor RMS de la señal de emisiones acústicas en dominio tiempo y el valor RMS de los coeficientes de la Transformada Discreta Wavelet (TDW usando la onda madre db6 de la misma señal acústica. La experimentación se realiza en un banco dedicado que permite cavitar a una bomba de ½ hp en distintos niveles de severidad y bajo diferentes condiciones de bombeo. Resultados experimentales mostraron que los indicadores propuestos permiten detectar y evaluar cualitativamente los niveles de severidad de la cavitación en una bomba centrífuga.In this paper an acoustic emission based cavitation fault detection system is proposed for a centrifugal pump. The monitoring is performed tracking a fault indicator obtained using the RMS value of the acoustic emission signal in time domain and the RMS value of the coefficients obtained by applying discrete wavelet transform on the acoustic signal using db6 mother wavelet. Experiments in different cavitation levels and under different operation conditions are carried out in a ½ hp centrifugal pump dedicated test bed. Results showed that the proposed fault indicators are suitable for detecting and evaluating cavitation severities in a centrifugal pump.

  12. Identification of bacteria in drinking and purified water during the monitoring of a typical water purification system

    Directory of Open Access Journals (Sweden)

    Mazzola Priscila

    2002-08-01

    Full Text Available Abstract Background A typical purification system that provides purified water which meets ionic and organic chemical standards, must be protected from microbial proliferation to minimize cross-contamination for use in cleaning and preparations in pharmaceutical industries and in health environments. Methodology Samples of water were taken directly from the public distribution water tank at twelve different stages of a typical purification system were analyzed for the identification of isolated bacteria. Two miniature kits were used: (i identification system (api 20 NE, Bio-Mérieux for non-enteric and non-fermenting gram-negative rods; and (ii identification system (BBL crystal, Becton and Dickson for enteric and non-fermenting gram-negative rods. The efficiency of the chemical sanitizers used in the stages of the system, over the isolated and identified bacteria in the sampling water, was evaluated by the minimum inhibitory concentration (MIC method. Results The 78 isolated colonies were identified as the following bacteria genera: Pseudomonas, Flavobacterium and Acinetobacter. According to the miniature kits used in the identification, there was a prevalence of isolation of P. aeruginosa 32.05%, P. picketti (Ralstonia picketti 23.08%, P. vesiculares 12.82%,P. diminuta 11.54%, F. aureum 6.42%, P. fluorescens 5.13%, A. lwoffi 2.56%, P. putida 2.56%, P. alcaligenes 1.28%, P. paucimobilis 1.28%, and F. multivorum 1.28%. Conclusions We found that research was required for the identification of gram-negative non-fermenting bacteria, which were isolated from drinking water and water purification systems, since Pseudomonas genera represents opportunistic pathogens which disperse and adhere easily to surfaces, forming a biofilm which interferes with the cleaning and disinfection procedures in hospital and industrial environments.

  13. Bacterial-based systems for expression and purification of recombinant Lassa virus proteins of immunological relevance

    Directory of Open Access Journals (Sweden)

    Cashman Kathleen A

    2008-06-01

    Full Text Available Abstract Background There is a significant requirement for the development and acquisition of reagents that will facilitate effective diagnosis, treatment, and prevention of Lassa fever. In this regard, recombinant Lassa virus (LASV proteins may serve as valuable tools in diverse antiviral applications. Bacterial-based systems were engineered for expression and purification of recombinant LASV nucleoprotein (NP, glycoprotein 1 (GP1, and glycoprotein 2 (GP2. Results Full-length NP and the ectodomains of GP1 and GP2 were generated as maltose-binding protein (MBP fusions in the Rosetta strains of Escherichia coli (E. coli using pMAL-c2x vectors. Average fusion protein yields per liter of culture for MBP-NP, MBP-GP1, and MBP-GP2 were 10 mg, 9 mg, and 9 mg, respectively. Each protein was captured from cell lysates using amylose resin, cleaved with Factor Xa, and purified using size-exclusion chromatography (SEC. Fermentation cultures resulted in average yields per liter of 1.6 mg, 1.5 mg, and 0.7 mg of purified NP, GP1 and GP2, respectively. LASV-specific antibodies in human convalescent sera specifically detected each of the purified recombinant LASV proteins, highlighting their utility in diagnostic applications. In addition, mouse hyperimmune ascitic fluids (MHAF against a panel of Old and New World arenaviruses demonstrated selective cross reactivity with LASV proteins in Western blot and enzyme-linked immunosorbent assay (ELISA. Conclusion These results demonstrate the potential for developing broadly reactive immunological assays that employ all three arenaviral proteins individually and in combination.

  14. Purification of Water by Aquatic Plants

    OpenAIRE

    Morimitsu, Katsuhito; Kawahigashi, Tatsuo

    2013-01-01

    [Abstract] Water quality purification of many water systems including those occurring in rivers depends to a great degree on water quality purification activities of aquatic plants and microbes. This paper presents a discussion of results, based on laboratory experiments, of purification by aquatic plants.

  15. Large scale expression and purification of mouse melanopsin-L in the baculovirus expression system

    NARCIS (Netherlands)

    Shirzad-Wasei, N.; Oostrum, J. van; Bovee-Geurts, P.H.M.; Wasserman, M.; Bosman, G.J.C.G.M.; Degrip, W.J.

    2013-01-01

    Melanopsin is the mammalian photopigment that primarily mediates non-visual photoregulated physiology. So far, this photopigment is poorly characterized with respect to structure and function. Here, we report large-scale production and purification of the intact long isoform of mouse melanopsin (mel

  16. FastTrack to supercritical fluid chromatographic purification: Implementation of a walk-up analytical supercritical fluid chromatography/mass spectrometry screening system in the medicinal chemistry laboratory.

    Science.gov (United States)

    Aurigemma, Christine; Farrell, William

    2010-09-24

    Medicinal chemists often depend on analytical instrumentation for reaction monitoring and product confirmation at all stages of pharmaceutical discovery and development. To obtain pure compounds for biological assays, the removal of side products and final compounds through purification is often necessary. Prior to purification, chemists often utilize open-access analytical LC/MS instruments because mass confirmation is fast and reliable, and the chromatographic separation of most sample constituents is sufficient. Supercritical fluid chromatography (SFC) is often used as an orthogonal technique to HPLC or when isolation of the free base of a compound is desired. In laboratories where SFC is the predominant technique for analysis and purification of compounds, a reasonable approach for quickly determining suitable purification conditions is to screen the sample against different columns. This can be a bottleneck to the purification process. To commission SFC for open-access use, a walk-up analytical SFC/MS screening system was implemented in the medicinal chemistry laboratory. Each sample is automatically screened through six column/method conditions, and on-demand data processing occurs for the chromatographers after each screening method is complete. This paper highlights the "FastTrack" approach to expediting samples through purification.

  17. Partial purification of the 5-hydroxytryptophan-reuptake system from human blood platelets using a citalopram-derived affinity resin

    Energy Technology Data Exchange (ETDEWEB)

    Biessen, E.A.L; Horn, A.S.; Robillard, G.T. (Univ. of Groningen (Netherlands))

    1990-04-03

    This paper describes a procedure for the synthesis and application of a citalopram-derived affinity resin in purifying the 5HT-reuptake system from human blood platelets. A two-step scheme has been developed for partial purification, based on wheat germ agglutinin-lectin (WGA) affinity and citalopram affinity chromatographies. Upon solubilization of the carrier with 1% digitonin, a 50-70-fold increase in specific ({sup 3}H) imipramine binding activity with a 70% recovery could be accomplished through WGA-lectin chromatography. The WGA pool was then subjected to affinity chromatography on citalopram-agarose. At least 90% of the binding capacity adsorbed to the column. Specific elution using 10 {mu}M citalopram resulted in a 22% recovery of binding activity. A 10,000-fold overall purification was obtained by using this two-step procedure. Analysis of the fractions on SDS-PAGE after {sup 125}I labeling revealed specific elution of 78- and 55-kDa proteins concomitant with the appearance of ({sup 3}H) imipramine binding activity. The pharmacological profile of the partially purified reuptake system correlated well with that derived from the crude membrane-bound reuptake system, suggesting a copurification of the 5HT binding activity and ({sup 3}H)imipramine binding activity.

  18. Disposable on-chip microfluidic system for buccal cell lysis, DNA purification, and polymerase chain reaction.

    Science.gov (United States)

    Cho, Woong; Maeng, Joon-Ho; Ahn, Yoomin; Hwang, Seung Yong

    2013-09-01

    This paper reports the development of a disposable, integrated biochip for DNA sample preparation and PCR. The hybrid biochip (25 × 45 mm) is composed of a disposable PDMS layer with a microchannel chamber and reusable glass substrate integrated with a microheater and thermal microsensor. Lysis, purification, and PCR can be performed sequentially on this microfluidic device. Cell lysis is achieved by heat and purification is performed by mechanical filtration. Passive check valves are integrated to enable sample preparation and PCR in a fixed sequence. Reactor temperature is needed to lysis and PCR reaction is controlled within ±1°C by PID controller of LabVIEW software. Buccal epithelial cell lysis, DNA purification, and SY158 gene PCR amplification were successfully performed on this novel chip. Our experiments confirm that the entire process, except the off-chip gel electrophoresis, requires only approximately 1 h for completion. This disposable microfluidic chip for sample preparation and PCR can be easily united with other technologies to realize a fully integrated DNA chip.

  19. Optimización de la geometría de la bomba hidráulica tipo jet

    Directory of Open Access Journals (Sweden)

    Vinicio Melo

    2010-12-01

    Full Text Available Español:Este trabajo presenta un método de cálculo directo para determinar la geometría óptima de una bomba jet para una aplicación determinada. Se revisan los conceptos necesarios del bombeo hidráulico tipo jet para poder explicar el procedimiento de dimensionamiento. El método usa las curvas de comportamiento de las bombas jet. Se calcula un área de tobera y se selecciona una relación de áreas. Esta información se usa para seleccionar una de las geometrías dadas por cualquier fabricante. Se presenta la secuencia de cálculos junto con un ejemplo de aplicación.

  20. Biogas recirculation for simultaneous calcium removal and biogas purification within an expanded granular sludge bed system treating leachate.

    Science.gov (United States)

    Luo, Jinghuan; Lu, Xueqin; Liu, Jianyong; Qian, Guangren; Lu, Yongsheng

    2014-12-01

    Biogas, generated from an expanded granular sludge bed (EGSB) reactor treating municipal solid waste (MSW) leachate, was recirculated for calcium removal from the leachate via a carbonation process with simultaneous biogas purification. Batch trials were performed to optimize the solution pH and imported biogas (CO2) for CaCO3 precipitation. With applicable pH of 10-11 obtained, continuous trials achieved final calcium concentrations of 181-375 mg/L (removal efficiencies≈92.8-96.5%) in the leachate and methane contents of 87.1-91.4% (purification efficiencies≈65.4-82.2%) in the biogas. Calcium-balance study indicates that 23-986 mg Ca/d was released from the bio-system under the carbonized condition where CaCO3 precipitating was moved outside the bioreactor, whereas 7918-9517 mg Ca/d was trapped into the system for the controlled one. These findings demonstrate that carbonation removal of calcium by biogas recirculation could be a promising alternative to pretreat calcium-rich MSW leachate and synergistically to improve methane content.

  1. A Hybrid MPC-PID Control System Design for the Continuous Purification and Processing of Active Pharmaceutical Ingredients

    Directory of Open Access Journals (Sweden)

    Maitraye Sen

    2014-05-01

    Full Text Available In this work, a hybrid MPC (model predictive control-PID (proportional-integral-derivative control system has been designed for the continuous purification and processing framework of active pharmaceutical ingredients (APIs. The specific unit operations associated with the purification and processing of API have been developed from first-principles and connected in a continuous framework in the form of a flowsheet model. These integrated unit operations are highly interactive along with the presence of process delays. Therefore, a hybrid MPC-PID is a promising alternative to achieve the desired control loop performance as mandated by the regulatory authorities. The integrated flowsheet model has been simulated in gPROMSTM (Process System Enterprise, London, UK. This flowsheet model has been linearized in order to design the control scheme. The ability to track the set point and reject disturbances has been evaluated. A comparative study between the performance of the hybrid MPC-PID and a PID-only control scheme has been presented. The results show that an enhanced control loop performance can be obtained under the hybrid control scheme and demonstrate that such a scheme has high potential in improving the efficiency of pharmaceutical manufacturing operations.

  2. Determinación de los parámetros de funcionamiento de bombas y motores hidráulicos

    Directory of Open Access Journals (Sweden)

    Luciano Pérez Sobrevilla

    2009-01-01

    Full Text Available Se presenta el manual de usuario del software "Performance Curves" para la determinación teórica y rápida de los parámetros de funcionamiento de las máquinas hidráulicas (bombas y motores, evitando la tediosa y lenta evaluación experimental e incrementando la eficiencia de la actividad con la menor cantidad de personal. El software posibilita determinar los parámetros adecuados de los regímenes óptimos de trabajo de la máquina hidráulica, las relaciones entre la eficiencia de la bomba y del motor hidráulico y un coeficiente adimensional de isogonalidad, que es un criterio de la semejanza cinemática de las máquinas hidráulicas rotatorias semejante geométricamente, así como una magnitud que caracteriza el régimen de trabajo de la máquina hidráulica dada. Las curvas de las eficiencias totales de la bomba y el motor hidráulico tienen el valor máximo para ciertos valores del coeficiente de isogonalidad, asegurando una precisión bastante alta, obteniéndose los valores máximos de la eficiencia de la máquina hidráulica.

  3. Desarrollo de una bomba reciprocante de doble efecto para desalinización de agua por medio de osmosis inversa

    Directory of Open Access Journals (Sweden)

    Carlos Julián Ospina

    2008-05-01

    Full Text Available El presente artículo describe el trabajo realizado en el desarrollo de un sistema de bombeo para desalinización con membranas de osmosis inversa. El sistema debe cumplir con requerimientos de alta presión (4.8 MPa y bajos caudales (3.78 L/min, para operar la membrana de osmosis inversa en su rango de mínimo consumo energético. Para cumplir este objetivo se desarrolla un sistema de desplazamiento positivo de acción simple y doble efecto, impulsado con un motoreductor cicloidal. La bomba desarrollada es evaluada experimentalmente bajo condiciones de laboratorio. El análisis de los resultados evidencia altas eficiencias de conversión de energía a lo largo del ciclo de bombeo. Posteriormente, el sistema de bombeo es evaluado en conjunto con una membrana de osmosis inversa, obteniéndose resultados satisfactorios en cuanto a tasas de flujo y concentraciones de permeado. El diseño cumple con los requisitos para sustituir sistemas de bombeo usados en la actualidad, tecnológicamente más complejos y al mismo tiempo más costosos./ The present paper describes the work carried out in the development of a pump system for water desalination in a small reverse osmosis plant. The system must satisfy high pressure (4.8 MPa and low flow rates (3.78 L/min to operate the reverse osmosis membrane in its minimum energy consumption range. In order to achieve this objective, a positive displacement pump system (single action double effect driven by a cyclo speed reduction was developed. The system was tested under laboratory conditions. The analysis of the results shows high energy conversion efficiencies throughout the pumping cycle. The equipment was validated based on the acceptable flow and permeates concentration results. This design is able of replacing actual pump systems that are technologically complex and at the same time more expensive.

  4. Improvement of the Purification System of Scrubbing Tower%洗涤塔净化系统的改进

    Institute of Scientific and Technical Information of China (English)

    赵英群

    2013-01-01

    详细介绍了本钢5#高炉煤气净化系统的老洗涤工艺与生产中的不利因素,根据生产的需要对排水系统和液压控制系统进行了改进,新改进的系统在实际应用中相比原系统提高了工作效率。%The negative factors in the original scrubbing process and production of the gas purification system of Benxi Steel’s No.5 blast furnace are introduced. The water drainage and hydraulic control systems were renovated according to the requirement of production; and the improved systems performed more efficiently compared to the old ones.

  5. Telomerase repeat amplification protocol (TRAP) activity upon recombinant expression and purification of human telomerase in a bacterial system.

    Science.gov (United States)

    Hansen, Debra T; Thiyagarajan, Thirumagal; Larson, Amy C; Hansen, Jeffrey L

    2016-07-01

    Telomerase biogenesis is a highly regulated process that solves the DNA end-replication problem. Recombinant expression has so far been accomplished only within a eukaryotic background. Towards structural and functional analyses, we developed bacterial expression of human telomerase. Positive activity by the telomerase repeat amplification protocol (TRAP) was identified in cell extracts of Escherichia coli expressing a sequence-optimized hTERT gene, the full-length hTR RNA with a self-splicing hepatitis delta virus ribozyme, and the human heat shock complex of Hsp90, Hsp70, p60/Hop, Hsp40, and p23. The Hsp90 inhibitor geldanamycin did not affect post-assembly TRAP activity. By various purification methods, TRAP activity was also obtained upon expression of only hTERT and hTR. hTERT was confirmed by tandem mass spectrometry in a ∼120 kDa SDS-PAGE fragment from a TRAP-positive purification fraction. TRAP activity was also supported by hTR constructs lacking the box H/ACA small nucleolar RNA domain. End-point TRAP indicated expression levels within 3-fold of that from HeLa carcinoma cells, which is several orders of magnitude below detection by the direct assay. These results represent the first report of TRAP activity from a bacterium and provide a facile system for the investigation of assembly factors and anti-cancer therapeutics independently of a eukaryotic setting.

  6. Expression and purification of the matrix protein of Nipah virus in baculovirus insect cell system.

    Science.gov (United States)

    Masoomi Dezfooli, Seyedehsara; Tan, Wen Siang; Tey, Beng Ti; Ooi, Chien Wei; Hussain, Siti Aslina

    2016-01-01

    Nipah virus (NiV) causes fatal respiratory illness and encephalitis in humans and animals. The matrix (M) protein of NiV plays an important role in the viral assembly and budding process. Thus, an access to the NiV M protein is vital to the design of viral antigens as diagnostic reagents. In this study, recombinant DNA technology was successfully adopted in the cloning and expression of NiV M protein. A recombinant expression cassette (baculovirus expression vector) was used to encode an N-terminally His-tagged NiV M protein in insect cells. A time-course study demonstrated that the highest yield of recombinant M protein (400-500 μg) was expressed from 107 infected cells 3 days after infection. A single-step purification method based on metal ion affinity chromatography was established to purify the NiV M protein, which successfully yielded a purity level of 95.67% and a purification factor of 3.39. The Western blotting and enzyme-linked immunosorbent assay (ELISA) showed that the purified recombinant M protein (48 kDa) was antigenic and reacted strongly with the serum of a NiV infected pig.

  7. Evaluación de dos bombas hidráulicas de engranajes de desplazamientos 32 y 50 cm3/rev

    Directory of Open Access Journals (Sweden)

    Pedro Paneque Rondón

    2013-01-01

    Full Text Available Como parte de las investigaciones que se llevan a cabo en la Universidad Agraria de La Habana (UNAH, en el Centro de Mecanización Agro- pecuaria (CEMA, con la cooperación de la Universidad Autónoma Chapingo (UACh, en el Departamento de Ingeniería Mecánica Agrícola (DIMA, dirigidas al desarrollo de la Oleohidráulica, se sometieron a evaluación dos bombas de engranajes de la marca Caproni de fabricación búlgara, con desplazamientos o cilindradas de 32 y 50 cm3/rev, refiriéndose la prueba a estas dos en específico y no a una muestra estadística de bombas de estos tipos. El objetivo del trabajo fue determinar las características técnicas de las referidas bombas. La eficiencia volumétrica que es el indicador que mide las fugas o pérdidas de caudal, en las dos bombas, fue superior al 90%. La potencia hidráulica para las presiones indicadas y frecuencia de rotación nominal, arroja valores aceptables de 11,32 kW para la de desplazamiento 32 cm3/rev y 17,60 kW para la de desplazamiento 50 cm3/rev. Las bombas evaluadas cumplen satisfactoriamente con las características técnicas presentadas por el fabricante.

  8. The influence of molecular weight of polyethylene glycol on separation and purification of pectinases from Penicillium cyclopium in aqueous two-phase system

    Directory of Open Access Journals (Sweden)

    Prodanović Jelena M.

    2008-01-01

    Full Text Available In this study the possibility of the partitioning and purification of pectinases from Penicillium cyclopium by their partitioning in polymer/polymer and polymer/salt aqueous two-phase systems was investigated. In the system with 10% (w/w polyethylene glycol 1500/5% (w/w dextran 500 000/85% (w/w crude enzyme, the highest values for partitioning parameters were achieved - the partition coefficient was 2.11, followed by the top phase yield of 85.68% and purification factor 1.28 for the endo-pectinase activity. The partition coefficient, yield in the top phase and purification factor for the exo-pectinase activity in the same system were 1.89, 84.28% and 3.82, respectively. In the system with 10% (w/w polyethylene glycol 6000/15% (w/w (NH42SO4/75% (w/w crude enzyme purification factor 37.85 for exo-pectinase, and 19.52 for endo-pectinase in the bottom phase were obtained.

  9. Direct Purification of Pectinase from Mango (Mangifera Indica Cv. Chokanan Peel Using a PEG/Salt-Based Aqueous Two Phase System

    Directory of Open Access Journals (Sweden)

    Abdul Manap Mohd Yazid

    2011-10-01

    Full Text Available An Aqueous Two-Phase System (ATPS was employed for the first time for the separation and purification of pectinase from mango (Mangifera Indica Cv. Chokanan peel. The effects of different parameters such as molecular weight of the polymer (polyethylene glycol, 2,000–10,000, potassium phosphate composition (12–20%, w/w, system pH (6–9, and addition of different concentrations of neutral salts (0–8%, w/w on partition behavior of pectinase were investigated. The partition coefficient of the enzyme was decreased by increasing the PEG molecular weight. Additionally, the phase composition showed a significant effect on purification factor and yield of the enzyme. Optimum conditions for purification of pectinase from mango peel were achieved in a 14% PEG 4000-14% potassium phosphate system using 3% (w/w NaCl addition at pH 7.0. Based on this system, the purification factor of pectinase was increased to 13.2 with a high yield of (97.6%. Thus, this study proves that ATPS can be an inexpensive and effective method for partitioning of pectinase from mango peel.

  10. Direct purification of pectinase from mango (Mangifera Indica Cv. Chokanan) peel using a PEG/salt-based Aqueous Two Phase System.

    Science.gov (United States)

    Mehrnoush, Amid; Sarker, Md Zaidul Islam; Mustafa, Shuhaimi; Yazid, Abdul Manap Mohd

    2011-10-10

    An Aqueous Two-Phase System (ATPS) was employed for the first time for the separation and purification of pectinase from mango (Mangifera Indica Cv. Chokanan) peel. The effects of different parameters such as molecular weight of the polymer (polyethylene glycol, 2,000-10,000), potassium phosphate composition (12-20%, w/w), system pH (6-9), and addition of different concentrations of neutral salts (0-8%, w/w) on partition behavior of pectinase were investigated. The partition coefficient of the enzyme was decreased by increasing the PEG molecular weight. Additionally, the phase composition showed a significant effect on purification factor and yield of the enzyme. Optimum conditions for purification of pectinase from mango peel were achieved in a 14% PEG 4000-14% potassium phosphate system using 3% (w/w) NaCl addition at pH 7.0. Based on this system, the purification factor of pectinase was increased to 13.2 with a high yield of (97.6%). Thus, this study proves that ATPS can be an inexpensive and effective method for partitioning of pectinase from mango peel.

  11. La cirugía coronaria sin bomba se asocia a peor permeabilidad de injertos arteriales y venosos y menor revascularización efectiva: Resultados del estudio Veterans Affairs Randomized On/Off Bypass

    Directory of Open Access Journals (Sweden)

    José María Melero

    2013-04-01

    Conclusiones: La CRC sin bomba obtuvo unas cifras de permeabilidad FitzGibbon A para injertos arteriales y venosos significativamente menores, y una revascularización efectiva también menor al compararla con la CRC con bomba. Al año, los pacientes con revascularización menos efectiva tuvieron una frecuencia mayor de eventos adversos.

  12. Validación Experimental de una Herramienta de Simulación de Bombas de Calor Experimental Validation of a Heat Pumps Simulation Tool

    Directory of Open Access Journals (Sweden)

    J.I. Linares

    2005-01-01

    Full Text Available Se ha desarrollado una herramienta informática que permite llevar a cabo el diseño y determinar la operación de bombas de calor por compresión mecánica de tipo "partido" (split. Después de analizar los modelos de la herramienta y de la interfase de la misma se procedió a llevar a cabo una validación experimental sobre dos bancos didácticos: un equipo de aire acondicionado aire/aire y una bomba de calor aire/agua. Esto ha permitido validar la bondad del modelo tanto para intercambiadores aire/refrigerante como para agua/refrigerante, incrementando así la versatilidad al modelo. Los resultados obtenidos permiten validar de forma satisfactoria la herramienta informática, siendo las desviaciones del modelo respecto a los valores reales inferiores al 20% en el peor caso, lo que permite emplear la herramienta tanto para optimizar el diseño de este tipo de equipos como para validar estrategias de control conducentes a la mejora de la eficienciaA software tool has been developed which permits developing the design (dimensioned and operation (functioning outside the nominal point of mechanical compression heat pumps of the "split" type. After analyzing the tool models and of the tool interface, experimental validation was done using two training benches: an air conditioning unit (air/air and a heat pump (air/water. This has allowed validation of the accuracy of the model for both air/refrigerant and water/refrigerant systems, thus increasing the versatility of the model. The results obtained have allowed a successful validation of the software, with deviations from model to real values of less than 20% in the worse case. This has allowed the use of the tool both for optimization of the design of these types of equipment, and for validating control strategies in order to improve their efficiency.

  13. Energy efficiency standardizing in vertical pumps; Normalizacion de eficiencia energetica en bombas verticales

    Energy Technology Data Exchange (ETDEWEB)

    Acosta Torres, Rosa Aracely; Buendia Dominguez, Eduardo H. [Instituto de Investigaciones Electricas, Cuernavaca (Mexico)

    1996-12-31

    The Instituto de Investigaciones Electricas (IIE) supporting the Comision Nacional para el Ahorro de Energia (CONAE) (National Commission for Energy Saving) developed the project of a standard on energy efficiency of vertical pumps, turbine type with external motor. This documents has fulfilled the necessary formalities for the issuing of a norm, being published the Mexican Official Norm (NOM-001-ENER-1995) on December 22, 1995. This article relates the results of the study for the elaboration of this norm, considering the energy saving, as well as the economic evaluation that supports the project of the norm, indicating also the environmental impact that its implantation will have. [Espanol] El Instituto de Investigaciones Electricas (IIE) apoyando a la Comision Nacional para el Ahorro de Energia (CONAE) elaboro el anteproyecto de norma de eficiencia energetica en bombas verticales tipo turbina con motor externo. Dicho documento ha cumplido con los tramites necesarios para la emision de una norma, habiendose publicado como Norma Oficial Mexicana (NOM-001-ENER-1995) el 22 de diciembre de 1995. En el presente articulo se mencionan los resultados del estudio para la realizacion de esta norma, considerando los ahorros energeticos, asi como la evaluacion economica que sustenta el proyecto de norma, indicando tambien el impacto ambiental que tendra su implantacion.

  14. Optimization of serine protease purification from mango (Mangifera indica cv. Chokanan) peel in polyethylene glycol/dextran aqueous two phase system.

    Science.gov (United States)

    Mehrnoush, Amid; Mustafa, Shuhaimi; Sarker, Md Zaidul Islam; Yazid, Abdul Manap Mohd

    2012-01-01

    Mango peel is a good source of protease but remains an industrial waste. This study focuses on the optimization of polyethylene glycol (PEG)/dextran-based aqueous two-phase system (ATPS) to purify serine protease from mango peel. The activity of serine protease in different phase systems was studied and then the possible relationship between the purification variables, namely polyethylene glycol molecular weight (PEG, 4000-12,000 g·mol(-1)), tie line length (-3.42-35.27%), NaCl (-2.5-11.5%) and pH (4.5-10.5) on the enzymatic properties of purified enzyme was investigated. The most significant effect of PEG was on the efficiency of serine protease purification. Also, there was a significant increase in the partition coefficient with the addition of 4.5% of NaCl to the system. This could be due to the high hydrophobicity of serine protease compared to protein contaminates. The optimum conditions to achieve high partition coefficient (84.2) purification factor (14.37) and yield (97.3%) of serine protease were obtained in the presence of 8000 g·mol(-1) of PEG, 17.2% of tie line length and 4.5% of NaCl at pH 7.5. The enzymatic properties of purified serine protease using PEG/dextran ATPS showed that the enzyme could be purified at a high purification factor and yield with easy scale-up and fast processing.

  15. Enhancement of a novel extracellular uricase production by media optimization and partial purification by aqueous three-phase system.

    Science.gov (United States)

    Ram, Senthoor K; Raval, Keyur; JagadeeshBabu, P E

    2015-01-01

    Uricase (urate: oxygen oxidoreductase, EC 1.7.3.3), an enzyme belonging to the class of oxidoreductases, catalyzes the enzymatic oxidation of uric acid to allantoin and finds a wide variety of application as therapeutic and clinical reagent. In this study, uricase production ability of the bacterial strains isolated from deep litter poultry soil is investigated. The strain with maximum extracellular uricase production capability was identified as Xanthomonas fuscans subsp. aurantifolii based on 16S rRNA sequencing. Effect of various carbon and nitrogen sources on uricase productivity was investigated. The uricase production for this strain was optimized using statistically based experimental designs and resulted in uricase activity of 306 U/L, which is 2 times higher than initial uricase activity. Two-step purification, such as ammonium sulfate precipitation and aqueous two-phase system, was carried out and a twofold increase in yield and specific activity was observed.

  16. Numerical studies of an eccentric tube-in-tube helically coiled heat exchanger for IHEP-ADS helium purification system

    CERN Document Server

    Zhang, Jianqin

    2014-01-01

    The tube-in-tube helically coiled (TTHC) heat exchanger is preferred in the purifier of IHEP-ADS helium purification system. The position of an internal tube is usually eccentric in a TTHC heat exchanger in practice, while most TTHC heat exchangers in the literature studied are concentric. In this paper, TTHC heat exchangers with different eccentricity ratios are numerically studied for turbulent flow and heat transfer characteristics under different flow rates. The fluid considered is helium at the pressure of 20Mpa, with temperature dependent thermo-physical properties for the inner tube and the annulus. The inner Nusselt number between the concentric and eccentric TTHC heat exchangers are compared, so is the annulus Nusselt number. The results show that with the eccentricity increasing, the annulus Nusselt number increases substantially. According to the numerical data, new empirical correlations of Nusselt number as a function of Reynolds number and eccentricity for the inner tube and the annulus are pres...

  17. Method and system for purification of gas/liquid streams for fuel cells or electrolysis cells

    DEFF Research Database (Denmark)

    2013-01-01

    The present invention provides in embodiments a method for purification of inlet gas/liquid streams in a fuel cell or electrolysis cell, the fuel cell or electrolysis cell comprising at least a first electrode, an electrolyte and a second electrode, the method comprising the steps of: - providing...... at least one scrubber in the gas/liquid stream at the inlet side of the first electrode of the fuel cell or electrolysis cell; and/or providing at least one scrubber in the gas/liquid stream at the inlet side of the second electrode of the fuel cell or electrolysis cell; and - purifying the gas...... with the at least one scrubber, with the proviso that the fuel cell or electrolysis cell is not a solid oxide cell....

  18. Purification of pectinase from mango (Mangifera indica L. cv. Chokanan) waste using an aqueous organic phase system: a potential low cost source of the enzyme.

    Science.gov (United States)

    Amid, Mehrnoush; Abdul Manap, Mohd Yazid; Mustafa, Shuhaimi

    2013-07-15

    As a novel method of purification, an aqueous organic phase system (AOPS) was employed to purify pectinase from mango waste. The effect of different parameters, such as the alcohol concentration (ethanol, 1-propanol, and 2-propanol), the salt type and concentration (ammonium sulfate, potassium phosphate and sodium citrate), the feed stock crude load, the aqueous phase pH and NaCl concentration, were investigated in the recovery of pectinase from mango peel. The partition coefficient (K), selectivity (S), purification factor (PF) and yield (Y, %) were investigated in this study as important parameters for the evaluation of enzyme recovery. The desirable partition efficiency for pectinase purification was achieved in an AOPS of 19% (w/w) ethanol and 22% (w/w) potassium phosphate in the presence of 5% (w/w) NaCl at pH 7.0. Based on the system, the purification factor of pectinase was enhanced 11.7, with a high yield of 97.1%. Copyright © 2013 Elsevier B.V. All rights reserved.

  19. Rapid, highly efficient extraction and purification of membrane proteins using a microfluidic continuous-flow based aqueous two-phase system.

    Science.gov (United States)

    Hu, Rui; Feng, Xiaojun; Chen, Pu; Fu, Meng; Chen, Hong; Guo, Lin; Liu, Bi-Feng

    2011-01-07

    Membrane proteins play essential roles in regulating various fundamental cellular functions. To investigate membrane proteins, extraction and purification are usually prerequisite steps. Here, we demonstrated a microfluidic aqueous PEG/detergent two-phase system for the purification of membrane proteins from crude cell extract, which replaced the conventional discontinuous agitation method with continuous extraction in laminar flows, resulting in significantly increased extraction speed and efficiency. To evaluate this system, different separation and detection methods were used to identify the purified proteins, such as capillary electrophoresis, SDS-PAGE and nano-HPLC-MS/MS. Swiss-Prot database with Mascot search engine was used to search for membrane proteins from random selected bands of SDS-PAGE. Results indicated that efficient purification of membrane proteins can be achieved within 5-7s and approximately 90% of the purified proteins were membrane proteins (the highest extraction efficiency reported up to date), including membrane-associated proteins and integral membrane proteins with multiple transmembrane domains. Compared to conventional approaches, this new method had advantages of greater specific surface area, minimal emulsification, reduced sample consumption and analysis time. We expect the developed method to be potentially useful in membrane protein purifications, facilitating the investigation of membrane proteomics.

  20. Estudio de la eficiencia operativa de las bombas eléctricas sumergibles (bes) en el campo vhr en base a las curvas de operación

    OpenAIRE

    2009-01-01

    Dada la gran necesidad de incrementar la producción en el distrito Amazónico, se propuso realizar un estudio de la eficiencia operativa de las bombas electrosumergible en el campo, en el cual se recopila toda la información relacionada con las bombas del campo, análisis de la información y determinación de posibles soluciones a las deficiencias operativas que permitan optimizar el proceso y obtener mayor producción de petróleo en el campo, que será de gran beneficio para la empresa y el país ...

  1. Influencia del contexto hidrogeológico en la eficiencia de los sistemas cerrados de bomba de calor

    OpenAIRE

    Folch Sancho, Albert; Goma Roca, Albert; Jimenez Parras, Santos; Zarroca HErnandez, Mario; Bach Plaza, Joan; Mas Pla, Josep

    2013-01-01

    Los sistemas geotérmicos de bomba de calor se caracterizan por presentar una elevada eficiencia energética en condiciones de óptimo funcionamiento. Aun así, su productividad se ve condicionada por las características geológicas e hidrogeológicas del medio. El presente estudio centra su interés en los sistemas cerrados verticales, dado que en muchos casos no se contabiliza el efecto del flujo de agua subterránea de forma adecuada. En este trabajo se evalúa el transporte de calor en 4 contextos...

  2. Ionic Liquid-Based Ultrasonic-Assisted Extraction of Secoisolariciresinol Diglucoside from Flaxseed (Linum usitatissimum L.) with Further Purification by an Aqueous Two-Phase System.

    Science.gov (United States)

    Tan, Zhi-Jian; Wang, Chao-Yun; Yang, Zi-Zhen; Yi, Yong-Jian; Wang, Hong-Ying; Zhou, Wan-Lai; Li, Fen-Fang

    2015-09-30

    In this work, a two-step extraction methodology of ionic liquid-based ultrasonic-assisted extraction (IL-UAE) and ionic liquid-based aqueous two-phase system (IL-ATPS) was developed for the extraction and purification of secoisolariciresinol diglucoside (SDG) from flaxseed. In the IL-UAE step, several kinds of ILs were investigated as the extractants, to identify the IL that affords the optimum extraction yield. The extraction conditions such as IL concentration, ultrasonic irradiation time, and liquid-solid ratio were optimized using response surface methodology (RSM). In the IL-ATPS step, ATPS formed by adding kosmotropic salts to the IL extract was used for further separation and purification of SDG. The most influential parameters (type and concentration of salt, temperature, and pH) were investigated to obtain the optimum extraction efficiency. The maximum extraction efficiency was 93.35% under the optimal conditions of 45.86% (w/w) IL and 8.27% (w/w) Na₂SO₄ at 22 °C and pH 11.0. Thus, the combination of IL-UAE and IL-ATPS makes up a simple and effective methodology for the extraction and purification of SDG. This process is also expected to be highly useful for the extraction and purification of bioactive compounds from other important medicinal plants.

  3. Method and system for purification of gas/liquid streams for fuel cells or electrolysis cells

    DEFF Research Database (Denmark)

    2013-01-01

    The present invention provides in embodiments a method for purification of inlet gas/liquid streams in a fuel cell or electrolysis cell, the fuel cell or electrolysis cell comprising at least a first electrode, an electrolyte and a second electrode, the method comprising the steps of: - providing...... at least one scrubber in the gas/liquid stream at the inlet side of the first electrode of the fuel cell or electrolysis cell; and/or providing at least one scrubber in the gas/liquid stream at the inlet side of the second electrode of the fuel cell or electrolysis cell; and - purifying the gas/liquid...... streams towards the first and second electrode; wherein the at least one scrubber in the gas/liquid stream at the inlet side of the first electrode and/or the at least one scrubber in the gas/liquid stream at the inlet side of the second electrode comprises a material suitable as an electrolyte material...

  4. Comportamiento no lineal y caótico en una bomba centrífuga operando en estado de cavitación//Nonlinear and chaotic behavior in a centrifugal pump operating in state of cavitation

    Directory of Open Access Journals (Sweden)

    Juan E. Álvarez Naranjo

    2015-05-01

    Full Text Available Mejorar la precisión en el diagnóstico y pronóstico del mantenimiento industrial ha sido una tarea de constante investigación debido a la necesidad de preservar el continuo funcionamiento de las máquinas de producción. En el presentetrabajo se estudió la bomba centrífuga en estado de cavitación. Se construyó un banco de pruebas y mediante obstrucción del fluido hacia el rodete del equipo por medio de la válvula de succión, se registraron las señales temporales mediante un acelerómetro. Posteriormente, se empleó un estudio no lineal y caótico para representar la geometría en el espacio de fases y su validación se realizó con el registro de datos de la bomba centrífuga operando sin cavitación y con máxima eficiencia. Los resultados mostraron que la dinámica del sistema actúa de forma no lineal y caótica, representado el fenómeno de cavitación con una geometría característica.Palabras claves: bomba centrífuga, cavitación, caos, dinámica no lineal, espacio de fases, serie temporal.______________________________________________________________________________AbstractImproving accuracy in the diagnosis and prognosis of industrial maintenance has been a constant task of research to preserve the continuous operation of machines. Today is necessary to improve this technique for avoiding reductionism that the traditional linear techniques employ. In this paper it is studied the centrifugal pump cavitation state. To simulate the phenomenon, a test bed is constructed and the fluid is blocked toward the impeller of pump by the suction valve, the time signals were recorded using an accelerometer. Subsequently, a chaotic nonlinear study was used to represent the geometry in the phase space and validation was performed with the data recording operation of the centrifugal pump without cavitation and with maximum efficiency. The results showed that the system dynamics is non-linear and chaotic, and the cavitation is represented

  5. An AIL/IL-based liquid/liquid extraction system for the purification of His-tagged proteins.

    Science.gov (United States)

    Xu, Weiyuan; Cao, Huazhen; Ren, Guangwei; Xie, Hujun; Huang, Jianying; Li, Shijun

    2014-06-01

    A sorbent based on affinity ionic liquid (AIL), triazacyclononane-ionic liquid, was synthesized, characterized, and applied to the extraction of histidine (His)-tagged proteins from aqueous buffer to ionic liquid (IL) phase. The adsorbed His-tagged proteins could be back-extracted from the IL phase to the aqueous buffer with an imidazole solution. The specific binding of His-tagged proteins with AIL/IL could be affected by a few factors including the ionic strength and coordinated metal ions. In the case of His-tagged enhanced green fluorescent protein (EGFP), the maximum binding capacity of Cu(2+)-AIL/IL reached 2.58 μg/μmol under the optimized adsorption conditions. The eluted His-tagged EGFP kept fluorescent and remained active through the purification process. Moreover, a tandem extraction process successively using Cu(2+)-AIL/IL and Zn(2+)-AIL/IL systems was developed, which was proven very efficient to obtain the ultimate protein with a purity of about 90 %. An effective reclamation method for the AIL/IL extraction system was further established. The sorbent could be easily regenerated by removing metal ions with EDTA and the followed reimmobilization of metal ions. Easy handling of the presented M(2+)-AIL/IL system and highly specific ability to absorb His-tagged proteins make it attractive and potentially applicable in biomolecular separation.

  6. A Novel Recombinant DNA System for High Efficiency Affinity Purification of Proteins in Saccharomyces cerevisiae

    Directory of Open Access Journals (Sweden)

    Brian H. Carrick

    2016-03-01

    Full Text Available Isolation of endogenous proteins from Saccharomyces cerevisiae has been facilitated by inserting encoding polypeptide affinity tags at the C-termini of chromosomal open reading frames (ORFs using homologous recombination of DNA fragments. Tagged protein isolation is limited by a number of factors, including high cost of affinity resins for bulk isolation and low concentration of ligands on the resin surface, leading to low isolation efficiencies and trapping of contaminants. To address this, we have created a recombinant “CelTag” DNA construct from which PCR fragments can be created to easily tag C-termini of S. cerevisiae ORFs using selection for a nat1 marker. The tag has a C-terminal cellulose binding module to be used in the first affinity step. Microgranular cellulose is very inexpensive and has an effectively continuous ligand on its surface, allowing rapid, highly efficient purification with minimal background. Cellulose-bound proteins are released by specific cleavage of an included site for TEV protease, giving nearly pure product. The tag can be lifted from the recombinant DNA construct either with or without a 13x myc epitope tag between the target ORF and the TEV protease site. Binding of CelTag protein fusions to cellulose is stable to high salt, nonionic detergents, and 1 M urea, allowing stringent washing conditions to remove loosely associated components, as needed, before specific elution. It is anticipated that this reagent could allow isolation of protein complexes from large quantities of yeast extract, including soluble, membrane-bound, or nucleic acid-associated assemblies.

  7. A Novel Recombinant DNA System for High Efficiency Affinity Purification of Proteins in Saccharomyces cerevisiae.

    Science.gov (United States)

    Carrick, Brian H; Hao, Linxuan; Smaldino, Philip J; Engelke, David R

    2015-12-29

    Isolation of endogenous proteins from Saccharomyces cerevisiae has been facilitated by inserting encoding polypeptide affinity tags at the C-termini of chromosomal open reading frames (ORFs) using homologous recombination of DNA fragments. Tagged protein isolation is limited by a number of factors, including high cost of affinity resins for bulk isolation and low concentration of ligands on the resin surface, leading to low isolation efficiencies and trapping of contaminants. To address this, we have created a recombinant "CelTag" DNA construct from which PCR fragments can be created to easily tag C-termini of S. cerevisiae ORFs using selection for a nat1 marker. The tag has a C-terminal cellulose binding module to be used in the first affinity step. Microgranular cellulose is very inexpensive and has an effectively continuous ligand on its surface, allowing rapid, highly efficient purification with minimal background. Cellulose-bound proteins are released by specific cleavage of an included site for TEV protease, giving nearly pure product. The tag can be lifted from the recombinant DNA construct either with or without a 13x myc epitope tag between the target ORF and the TEV protease site. Binding of CelTag protein fusions to cellulose is stable to high salt, nonionic detergents, and 1 M urea, allowing stringent washing conditions to remove loosely associated components, as needed, before specific elution. It is anticipated that this reagent could allow isolation of protein complexes from large quantities of yeast extract, including soluble, membrane-bound, or nucleic acid-associated assemblies.

  8. Cavitación y materiales de construcción en las bombas centrífugas

    Directory of Open Access Journals (Sweden)

    Rafael Pérez-Barreto

    2004-03-01

    Full Text Available Se propone, fundamenta y ejemplifica un procedimiento para determinar la carga neta de succión positiva con el fin de evaluar la calidad del proyecto de una bomba centrífuga, su fabricación y confiabilidad de los datos técnicos. Se muestran los gráficos para determinar el valor del coeficiente de velocidad específica de succión en dependencia de la velocidad específica para rodetes en voladizo y entre apoyos, y sus correspondientes correlaciones analíticas. Se resume la situación actual de los materiales de fabricación de las bombas centrífugas y su relación con los costos y la corrosión, así como la durabilidad del equipamiento con el objetivo de orientar a usuarios y fabricantes, especialmente de la industria del níquel, en la elección para diferentes condiciones y evaluar la corrosión en ausencia de cavitación.

  9. Sistema automatizado para la determinación del estado de maduración en fruta bomba

    Directory of Open Access Journals (Sweden)

    Minelkis Machado Molina

    2015-01-01

    Full Text Available El desarrollo de tecnologías digitales y su aplicación en la agricultura brindan nuevas posibilidades de automatización de procesos tecnológicos. La investigación realizada tiene como objetivo desarrollar una herramienta informática para la determinación no destructiva del estado de maduración en fruta bomba (var. Maradol Roja. Para alcanzar dicho objetivo se emplea la metodología para el diseño del software Proceso Unificado de Desarrollo (RUP. Para el procesamiento de imágenes se utilizan técnicas de reconocimiento de patrones y para la con - vención de modos de color de RGB a Lab se entrena una red neuronal Perceptron multicapas. Entre los principales resultados se presentan la elevada dependencia entre el IC* y el estado de maduración alcanzando un R 2 de 0,91. Se establecen rangos de IC* por estado de maduración, además se obtiene el software SADEM (Sistema Automatizado para Determinar Estado de Maduración que mediante una imagen digital establece el estado de maduración de la fruta bomba variedad Maradol Roja.

  10. Expression, stabilization and purification of membrane proteins via diverse protein synthesis systems and detergents involving cell-free associated with self-assembly peptide surfactants.

    Science.gov (United States)

    Zheng, Xuan; Dong, Shuangshuang; Zheng, Jie; Li, Duanhua; Li, Feng; Luo, Zhongli

    2014-01-01

    G-protein coupled receptors (GPCRs) are involved in regulating most of physiological actions and metabolism in the bodies, which have become most frequently addressed therapeutic targets for various disorders and diseases. Purified GPCR-based drug discoveries have become routine that approaches to structural study, novel biophysical and biochemical function analyses. However, several bottlenecks that GPCR-directed drugs need to conquer the problems including overexpression, solubilization, and purification as well as stabilization. The breakthroughs are to obtain efficient protein yield and stabilize their functional conformation which are both urgently requiring of effective protein synthesis system methods and optimal surfactants. Cell-free protein synthesis system is superior to the high yields and post-translation modifications, and early signs of self-assembly peptide detergents also emerged to superiority in purification of membrane proteins. We herein focus several predominant protein synthesis systems and surfactants involving the novel peptide detergents, and uncover the advantages of cell-free protein synthesis system with self-assembling peptide detergents in purification of functional GPCRs. This review is useful to further study in membrane proteins as well as the new drug exploration.

  11. Correlations for the prediction of the head curve of centrifugal pumps based on experimental data = Correlações para a predição da curva de altura de bombas centrífugas baseadas em dados experimentais

    OpenAIRE

    Natália Argene Lovate Pereira Fleischfresser

    2015-01-01

    A performance hidráulica de bombas centrífugas depende de várias entre suas dimensões hidráulicas, mas a maioria delas não é facilmente acessível. Por este motivo, a performance hidráulica da bomba deve sempre ser disponibilizada pelo seu fabricante. No entanto, para proteger sua propriedade intelectual, fabricantes raramente compartilham com o público informações mais detalhadas sobre a hidráulica da bomba. Como consequência, os usuários dos equipamentos e pesquisadores não têm acesso a toda...

  12. Avaliação e aperfeiçoamento de uma bomba de sangue centrífuga implantável ápice ventricular para assistência cardíaca

    OpenAIRE

    Bruno Utiyama da Silva

    2012-01-01

    Resumo: Neste trabalho, foi realizado um estudo experimental de uma Bomba de Sangue Ápice Ventricular (BSAV) para sua análise como um dispositivo de assistência ventricular. Este estudo faz parte do desenvolvimento desta bomba e serviu como fundamentação na evolução do projeto. A BSAV é uma bomba de sangue centrífuga de fluxo contínuo, para ser utilizada como dispositivo de assistência ventricular em pacientes com doenças cardíacas crônicas. Foi realizado um estudo de posicionamento anatômico...

  13. Purification and characterization of a thermostable glucoamylase ...

    African Journals Online (AJOL)

    STORAGESEVER

    2010-06-07

    Jun 7, 2010 ... tryptophan and serine residues in the catalytic process. Raw corn .... selected for amylase production, purification and characterization. .... chromatogram was developed with solvent system of butanol/ ... starch affinity and acetone precipitation method for ..... Optimization of extraction and purification of.

  14. Estudio y propuestas de solución para fallos recurrentes en bombas centrífugas horizontales // Study and proposal of solutions to recurrent failures in horizontal centrifugal pumps.

    Directory of Open Access Journals (Sweden)

    Jesús Cabrera - Gómez

    2010-05-01

    Full Text Available ResumenLa necesidad de ejecutar la investigación está dada por los fallos recurrentes que están presentandolas bombas centrífugas horizontales que interrumpen el proceso de la planta de alquilación y alimpacto económico asociado a los fallos que han tenido un elevado costo por acciones demantenimiento preventivas y reactivas, en adición a los costos asociados por pérdida deoportunidad. Se llevó a cabo un estudio para establecer las causas que originan los reiterados fallosocurridos en el sistema de bombeo. Como resultado del trabajo realizado, se identificaronoportunidades de mejora de la disponibilidad de los activos estudiados por la vía del incremento de lavida útil del los sellos mecánicos y finalmente se propuso un programa de acciones correctivas alque debe darse seguimiento y que debe tributar a la solución permanente de los problemasidentificados.Palabras claves: mantenimiento de equipos rotatorios, fallos recurrentes, confiabilidad operacional,_________________________________________________________AbstractThe necessity of making the research outlined in this paper is given by recurrent failures in a set ofhorizontal centrifugal pumps. These failures interrupt the operating process in an alkylation plant andgenerate a great negative economic impact because of incrementing maintenance costs andopportunity losses. A study was carried out in order to establish the main causes of repeated failuresin the pumping system. As a result, several improvement opportunities of availability of pumps wereidentified by increasing the useful life of their mechanical seals. Finally, a corrective action programwas proposed in order to provide permanent solutions to identified problems.Key words: maintenance of rotating equipment, recurrent failures, operational reliability, root causeanalysis, horizontal centrifugal pumps.análisis de causa raíz, bombas centrífugas horizontales

  15. The Brookside Farm Wetland Ecosystem Treatment (WET System: A Low-Energy Methodology for Sewage Purification, Biomass Production (Yield, Flood Resilience and Biodiversity Enhancement

    Directory of Open Access Journals (Sweden)

    Julian C. Abrahams

    2017-01-01

    Full Text Available Wastewater from domestic developments, farms and agro-industrial processing can be sources of pollution in the environment; current wastewater management methods are usually machine-based, and thus energy consuming. When Permaculture Principles are used in the creation of water purification and harvesting systems, there can be multiple environmental and economic benefits. In the context of energy descent, it may be considered desirable to treat wastewater using minimal energy. The constructed wetland design presented here is a low-entropy system in which wastewater is harvested and transformed into lush and productive wetland, eliminating the requirement for non-renewable energy in water purification, and also maximising benefits: biodiversity, flood resilience and yield. In permaculture design, the high concentrations of nitrogen and phosphorous compounds in sewage are viewed as valuable nutrients, resources to be harvested by a constructed wetland ecosystem and converted into useful yield. Similarly, rainwater runoff is not viewed as a problem which can cause flooding, but as a potential resource to be harvested to provide a yield. This paper presents a case study, with both water quality and productivity data, from Brookside Farm UK, where the use of Permaculture Design Principles has created a combined wastewater management and purification system, accepting all site water.

  16. Experimental study of the portable blood purification system for treatment of acute renal failure in dogs under the field condition in a high-altitude environment

    Directory of Open Access Journals (Sweden)

    Hai-bin LI

    2014-01-01

    Full Text Available Objective To reproduce the model of acute renal failure (ARF in beagles for comprehensively evaluating the safety, stability and validity of the continuous blood purification system under the field condition in a high-altitude environment. Methods Adult beagle ARF model was reproduced by bilateral nephrectomy. All ARF dogs underwent continuous veno-venous hemofiltration with a blood purification machine. Vital signs, renal function, liver function and plasma ion-levels before and after the therapy were analyzed through self-controlled study. Performance of the continuous blood purification machine running in a highaltitude environment was evaluated. Results Blood urea nitrogen (BUN and creatinine (Cr of six dogs were assayed 24 hours after nephrectomy, the difference showed statistical significance compared with those before the surgery (P<0.05, implying that six ARF models were reproduced successfully. Six dogs experienced continuous renal replacement therapy (CRRT with external jugular vein double-lumen tube placement (3 dogs or femoral vein catheter placement (3 dogs. Five dogs successfully finished the whole experiment, and one dog died because of falling off of the dialysis tube and blood loss. The continuous blood purification machine was sensitive with rapid release of warning signals when the condition was abnormal. Compared to that before CRRT, there were no statistically significant differences in heart rate, respiratory rate and blood pressure in ARF models 2, 4 hours after CRRT (P>0.05. And there were no statistically significant differences in ALT, AST and T-Bil in ARF models between that before and 2, 4 hours after CRRT (P>0.05. However, 2, 4 hours after CRRT, BUN and Cr levels descended significantly (P<0. 05, so was K+ level (P<0. 05. There were no complications such as hemorrhage or hypotension. There were no statistical differences in volume of replacement fluid or discarded fluid between before and after CRRT

  17. Chemical looping integration with a carbon dioxide gas purification unit

    Energy Technology Data Exchange (ETDEWEB)

    Andrus, Jr., Herbert E.; Jukkola, Glen D.; Thibeault, Paul R.; Liljedahl, Gregory N.

    2017-01-24

    A chemical looping system that contains an oxidizer and a reducer is in fluid communication with a gas purification unit. The gas purification unit has at least one compressor, at least one dryer; and at least one distillation purification system; where the gas purification unit is operative to separate carbon dioxide from other contaminants present in the flue gas stream; and where the gas purification unit is operative to recycle the contaminants to the chemical looping system in the form of a vent gas that provides lift for reactants in the reducer.

  18. Optimization of Serine Protease Purification from Mango (Mangifera indica cv. Chokanan Peel in Polyethylene Glycol/Dextran Aqueous Two Phase System

    Directory of Open Access Journals (Sweden)

    Abdul Manap Mohd Yazid

    2012-03-01

    Full Text Available Mango peel is a good source of protease but remains an industrial waste. This study focuses on the optimization of polyethylene glycol (PEG/dextran-based aqueous two-phase system (ATPS to purify serine protease from mango peel. The activity of serine protease in different phase systems was studied and then the possible relationship between the purification variables, namely polyethylene glycol molecular weight (PEG, 4000–12,000 g·mol−1, tie line length (−3.42–35.27%, NaCl (−2.5–11.5% and pH (4.5–10.5 on the enzymatic properties of purified enzyme was investigated. The most significant effect of PEG was on the efficiency of serine protease purification. Also, there was a significant increase in the partition coefficient with the addition of 4.5% of NaCl to the system. This could be due to the high hydrophobicity of serine protease compared to protein contaminates. The optimum conditions to achieve high partition coefficient (84.2 purification factor (14.37 and yield (97.3% of serine protease were obtained in the presence of 8000 g·mol−1 of PEG, 17.2% of tie line length and 4.5% of NaCl at pH 7.5. The enzymatic properties of purified serine protease using PEG/dextran ATPS showed that the enzyme could be purified at a high purification factor and yield with easy scale-up and fast processing.

  19. Design of aqueous two-phase systems for purification of hyaluronic acid produced by metabolically engineered Lactococcus lactis.

    Science.gov (United States)

    Rajendran, Vivek; Puvendran, Kirubhakaran; Guru, Bharath Raja; Jayaraman, Guhan

    2016-02-01

    Hyaluronic acid has a wide range of biomedical applications and its commercial value is highly dependent on its purity and molecular weight. This study highlights the utility of aqueous two-phase separation as a primary recovery step for hyaluronic acid and for removal of major protein impurities from fermentation broths. Metabolically engineered cultures of a lactate dehydrogenase mutant strain of Lactococcus lactis (L. lactis NZ9020) were used to produce high-molecular-weight hyaluronic acid. The cell-free fermentation broth was partially purified using a polyethylene glycol/potassium phosphate system, resulting in nearly 100% recovery of hyaluronic acid in the salt-rich bottom phase in all the aqueous two-phase separation experiments. These experiments were optimized for maximum removal of protein impurities in the polyethylene glycol rich top phase. The removal of protein impurities resulted in substantial reduction of membrane fouling in the subsequent diafiltration process, carried out with a 300 kDa polyether sulfone membrane. This step resulted in considerable purification of hyaluronic acid, without any loss in recovery and molecular weight. Diafiltration was followed by an adsorption step to remove minor impurities and achieve nearly 100% purity. The final hyaluronic acid product was characterized by Fourier-transform IR and NMR spectroscopy, confirming its purity.

  20. A Hydroponic System for Purification of Anaerobically Treated Dairy Manure and Production of Wheat as a Nutritional Forage Crop

    Directory of Open Access Journals (Sweden)

    Abdel E. Ghaly

    2007-01-01

    Full Text Available A hydroponic system was developed and used for purification of an anaerobically treated dairy manure and production of forage crops. The effect of light duration, seeding rate and wastewater application rate on the crop yield and pollution potential reduction were studied. The results indicated that a wheat forage crop can be produced in 21 days from germination to harvest in this system and removal efficiencies of up to 89.9, 94.6, and 86.7 % can be achieved for the total solids, chemical oxygen demand (COD and ammonium nitrogen, respectively. Increasing the wastewater application rate increased the crop yield and decreased the pollutants removal efficiencies. A treatment combination of wastewater application rate of 900 mL/day, a seeding rate of 400 g and a light duration of 12 hours gave the best results for crop yield (3.65 kg of wheat/tray. A total possible yield of 3160 tonnes per hectare per year can be achieved with the system (with thirteen harvests per year. This is more than 98 times greater than the yield obtainable from a field grown conventional forage of 245 tonnes per hectare per year. At the optimum forage production, removal efficiencies of 75.7, 85.9 and 75.6% were achieved for the solids, COD, ammonium nitrogen, respectively. A nitrate nitrogen concentration of 6.7 mg/L was found in the effluent from the hydroponic system. This is below the Canadian Environmental and Health Guidelines of 10 mg/L.

  1. System Aero-Accelator for the purification of biodegradable effluents; Sistema aero-accelator para la depuracion de efluentes biodegradables (I)

    Energy Technology Data Exchange (ETDEWEB)

    Bosque Hernandez, J. L. del; Martin Sanchez, J. L. [Universidad de Salamanca (Spain)

    2000-07-01

    The contamination of the waters is one of the factors that contributes to the deterioration of our environment and since it is a very limited one its treatment descontaminant it is one of the politic's main objectives and environmental administration at all the levels, being spread to the total purification of the generated residual effluents. To reach this objective, big technological efforts are required that allow next to the creation of new processes, the adaptation of the processes existent depuratives, increasing the effectiveness of the same ones. One of the techniques of purification of possible recovery is the Compact System of active mires Aero-Accelator. Presently work is designed and it builds a plant pilot with Aero-Accelator geometry to study its behavior in the treatment of effluents of urban type with different loads pollutants. (Author) 16 refs.

  2. Methodology to monitor and diagnostic vibrations of the motor-pumps used in the primary cooling system of IEAR-1 nuclear research reactor; Metodologia para monitoracao e diagnostico de vibracao das bombas moto-operadas do circuito primario de refrigeracao do Reator IEA-R1

    Energy Technology Data Exchange (ETDEWEB)

    Benevenuti, Erion de Lima

    2004-07-01

    The objectives of this study are to establish a strategy to monitor and diagnose vibrations of the motor pumps used in the primary reactor cooling system of the IEA-R1 nuclear research reactor, to verify the possibility of using the existing installed monitoring vibration system and to implement such strategy in a continuous way. Four types of mechanical problems were considered: unbalancing, misalignment, gaps and faults in bearings. An adequate set of analysis tools, well established by the industry, was selected. These are: global measurements of vibration, velocity spectrum and acceleration envelope spectrum. Three sources of data and information were used; the data measured from the primary pumps, experimental results obtained with a Spectra Quest machine used to simulate mechanical defects and data from the literature. The results show that, for the specific case of the motor-pumps of IEA-R1 nuclear research reactor, although the technique using the envelope of acceleration, which is not available in the current system used to monitor the vibration of the motor pumps, is the one with best performance, the other techniques available in the system are sufficient to monitor the four types of mechanical problems mentioned. The proposed strategy is shown and detailed in this work. (author)

  3. 天然气净化厂火炬系统设计研究%Design of the Flare System of Natural Gas Purification Plants

    Institute of Scientific and Technical Information of China (English)

    孙一伦

    2014-01-01

    The flare system design of natural gas purification plants is different from ordinary refineries. The flare gas in natural gas purification plants has high sulfur content and low temperature. And current purification plants constructed by Sinopec Group require device maintenance in batches, namely the flare system can not shut down, which will propose new design issues and requirements.%天然气净化厂火炬系统的设计与普通炼油厂火炬系统设计有所不同。天然气净化厂中的放空火炬气含硫量高、放空温度低。而且目前中石化所建设的净化厂项目均要求装置轮番检修,即火炬系统不能停工,对设计提出了新的课题和要求。

  4. A novel aqueous micellar two-phase system composed of surfactant and sorbitol for purification of pectinase enzyme from Psidium guajava and recycling phase components.

    Science.gov (United States)

    Amid, Mehrnoush; Murshid, Fara Syazana; Manap, Mohd Yazid; Hussin, Muhaini

    2015-01-01

    A novel aqueous two-phase system composed of a surfactant and sorbitol was employed for the first time to purify pectinase from Psidium guajava. The influences of different parameters, including the type and concentration of the surfactant and the concentration and composition of the surfactant/sorbitol ratio, on the partitioning behavior and recovery of pectinase were investigated. Moreover, the effects of system pH and the crude load on purification fold and the yield of purified pectinase were studied. The experimental results indicated that the pectinase was partitioned into surfactant-rich top phase, and the impurities were partitioned into the sorbitol-rich bottom phase with the novel method involving an ATPS composed of 26% (w/w) Triton X-100 and 23% (w/w) sorbitol at 54.2% of the TLL crude load of 20% (w/w) at pH 6.0. The enzyme was successfully recovered by this method with a high purification factor of 15.2 and a yield of 98.3%, whereas the phase components were also recovered and recycled at rates above 96%. This study demonstrated that this novel ATPS method can be used as an efficient and economical alternative to the traditional ATPS for the purification and recovery of the valuable enzyme.

  5. Model developed for an initiator event of loss of a system with five pumps shareable by two units of a plant. Comparative analysis of FCCs with simplified model APS; Modelo desarrollado para un suceso iniciador de perdida de un sistema con cinco bombas compartibles por las dos unidades de una plant. analisis comparativo de FCCs con modelo simplificado APS.

    Energy Technology Data Exchange (ETDEWEB)

    Lopez Lorenzo, M. A.; Perez Martin, F.

    2013-07-01

    In this paper is an analysis for a system with five pumps, each with 100% capacity, shared by two units of a plant, of which two are pre-assigned to each unit, one is common to both, and the possibility of assigning a pump of reserve of a unit to another unit. The purpose of this paper is to make a simplified model in which are taken into account all the possible combinations of independent failures and common cause, evaluating whether the inclusion in the complete model is required.

  6. [Isolation and purification of alpha-glycerophosphate oxidase in a polyethylene glycol/(NH4 )2SO4 aqueous two-phase system].

    Science.gov (United States)

    Meng, Yao; Jin, Jiagui; Liu, Shuangfeng; Yang, Min; Zhang, Qinglian; Wan, Li; Tang, Kun

    2014-02-01

    Alpha-glycerophosphate oxidase (alpha-GPO) from Enterococcus casseli flavus was successfully isolated and purified by using polyethylene glycol (PEG)/(NH4)2SO4 aqueous two-phase system (ATPS). The results showed that the chosen PEG/(NH4)2SO4 ATPS could be affected by PEG molecular weight, pH, concentration of PEG and (NH4)2SO4, and inorganic salt as well as additional amount of crude enzyme. After evaluating these influencing factors, the final optimum purification strategy was formed by 16.5% (m/m) PEG2000, 13.2% (m/m) (NH4)2SO4, pH 7.5 and 30% (m/m) additive crude enzyme, respectively. The NaCl was a negative influencing factor which would lead to lower purification fold and activity recovery. These conditions eventually resulted in the activity recovery of 89% (m/m), distribution coefficient of 1.2 and purification fold of 7.0.

  7. Development of a chamber system for rapid, high yield and cost-effective purification of deoxyribonucleic acid fragments from agarose gel

    Directory of Open Access Journals (Sweden)

    Gilda Eslami

    2014-01-01

    Full Text Available Background: There are several methods commonly practicing for deoxyribonucleic acid (DNA purification from agarose gel. In most laboratories, especially in developing countries, present methods for recovering of DNA fragments from the gel are mostly involved organic solvents. However, manual purification using organic solvents are toxic, labor intensive, time consuming and prone to contamination owing to several handling steps. The above mentioned burdens as well as cost and long time to import them, especially in developing countries, prompted us to design and develop a chamber system for rapid, non-toxic, cost-effective and user friendly device for polymerase chain reaction (PCR products purification from agarose gel. Materials and Methods: The device was made from plexiglass plates. After amplification of two fragments of 250 and 850 bp, PCR products were electrophoresed. Subsequently, the desired bands were excised and purified with three method: HiPer Mini chamber, phenol extraction method and spin column procedure. To assess the suitability of the purified DNAs, restriction digestion was applied. Results: Results showed that the yield of recovered DNA in our method was above 95%, whereas the yields obtained with conventional phenol extraction and spin column methods were around 60%. Conclusion: In conclusion, the current method for DNA elution is quick, inexpensive and robust and it does not require the use of toxic organic solvents. In addition, the purified DNA was well has suited for further manipulations such as restriction digestion, ligation, cloning, sequencing and hybridization.

  8. Air/Water Purification

    Science.gov (United States)

    1992-01-01

    After 18 years of research into air/water pollution at Stennis Space Center, Dr. B. C. Wolverton formed his own company, Wolverton Environmental Services, Inc., to provide technology and consultation in air and water treatment. Common houseplants are used to absorb potentially harmful materials from bathrooms and kitchens. The plants are fertilized, air is purified, and wastewater is converted to clean water. More than 100 U.S. communities have adopted Wolverton's earlier water hyacinth and artificial marsh applications. Catfish farmers are currently evaluating the artificial marsh technology as a purification system.

  9. Water Purification Product

    Science.gov (United States)

    2004-01-01

    Ecomaster, an affiliate of BioServe Space Technologies, this PentaPure technology has been used to purify water for our nation's Space Shuttle missions since 1981. WTC-Ecomaster of Mirneapolis, Minnesota manufactures water purification systems under the brand name PentaPure (TM). BioServe researcher Dr. George Marchin, of Kansas State University, first demonstrated the superiority of this technology and licensed it to WTC. Marchin continues to perform microgravity research in the development of new technologies for the benefit of life on Earth.

  10. Ionic liquids for two-phase systems and their application for purification, extraction and biocatalysis.

    Science.gov (United States)

    Oppermann, Sebastian; Stein, Florian; Kragl, Udo

    2011-02-01

    The development of biotechnological processes using novel two-phase systems based on molten salts known as ionic liquids (ILs) got into the focus of interest. Many new approaches for the beneficial application of the interesting solvent have been published over the last years. ILs bring beneficial properties compared to organic solvents like nonflammability and nonvolatility. There are two possible ways to use the ILs: first, the hydrophobic ones as a substitute for organic solvents in pure two-phase systems with water and second, the hydrophilic ones in aqueous two-phase systems (ATPS). To effectively utilise IL-based two-phase systems or IL-based ATPS in biotechnology, extensive experimental work is required to gain the optimal system parameters to ensure selective extraction of the product of interest. This review will focus on the most actual findings dealing with the basic driving forces for the target extraction in IL-based ATPS as well as presenting some selected examples for the beneficial application of ILs as a substitute for organic solvents. Besides the research focusing on IL-based two-phase systems, the "green aspect" of ILs, due to their negligible vapour pressure, is widely discussed. We will present the newest results concerning ecotoxicity of ILs to get an overview of the state of the art concerning ILs and their utilisation in novel two-phase systems in biotechnology.

  11. Lithium Propellant Purification and Filtration System For LFA and MPD Thrusters Project

    Data.gov (United States)

    National Aeronautics and Space Administration — Lithium has been proposed as an attractive metal propellant for advanced nuclear-electric propulsion missions in the outer solar system. While it is low molecular...

  12. The Doubly Oppressed: The Portrayal of Female Characters in Mongo Beti’s The Poor Christ of Bomba

    Directory of Open Access Journals (Sweden)

    Abubakar Mohammed Sani

    2015-05-01

    Full Text Available This essay explores and analysis the condition of female characters in Mongo Beti’s novel, The Poor Christ of Bomba(1971. The paper argues that women in the novel’s settings are being oppressed and unjustly maltreated by both colonialists, spearheaded by Father Drumont, and by the native African culture. Thus, the concept of “double oppression” is suitable in demonstrating the theme of maltreatment and exploitation of women in the novel, and their roles in the society. The paper exposes the pretence, hypocrisy, insincerity and biasness of the Christian mission in respect to women issues such polygamy, adultery and other sundry issues. Keywords: Female characters, the doubly oppressed, English literature

  13. Time, Temperature and Amount of Distilled Water Effects on the Purity and Yield of Bis(2-hydroxyethyl Terephthalate Purification System

    Directory of Open Access Journals (Sweden)

    H.W. Goh

    2015-07-01

    Full Text Available Polyethylene terephthalate (PET bottle is one of the common plastic wastes existed in the municipal solid waste in Malaysia. One alternative to solve the abundant of PET wastes is chemical recycling of the wastes to produce a value added product. This technology not only can decrease the PET wastes in landfill sites but also can produce many useful recycled PET products. Bis(2-hydroxyethyl terephthalate (BHET obtained from glycolysis reaction of PET waste was purified using crystallization process. The hot distilled water was added to glycolysis product followed by cooling and filtration to extract BHET in white solid form from the product. The effect of three operating conditions namely crystallization time, crystallization temperatures and amount of distilled water used to the yield of crystallization process were investigated. The purity of crystallization products were analyzed using HPLC and DSC. The optimum conditions of 3 hours crystallization time, 2 °C crystallization temperature and 5:1 mass ratio of distilled water used to glycolize solid gave the highest yield and purity of the crystallization process. © 2015 BCREC UNDIP. All rights reservedReceived: 12nd August 2014; Revised: 4th February 2015; Accepted: 5th February 2015How to Cite: Goh, H.W., Salmiaton, A., Abdullah, N., Idris, A. (2015. Time, Temperature and Amount of Distilled Water Effects on the Purity and Yield of Bis(2-hydroxyethyl Terephthalate Purification System. Bulletin of Chemical Reaction Engineering & Catalysis, 10 (2: 143-154. (doi:10.9767/bcrec.10.2.7195.143-154 Permalink/DOI: http://dx.doi.org/10.9767/bcrec.10.2.7195.143-154  

  14. A Novel Aqueous Two Phase System Composed of Surfactant and Xylitol for the Purification of Lipase from Pumpkin (Cucurbita moschata) Seeds and Recycling of Phase Components.

    Science.gov (United States)

    Amid, Mehrnoush; Manap, Mohd Yazid; Hussin, Muhaini; Mustafa, Shuhaimi

    2015-06-17

    Lipase is one of the more important enzymes used in various industries such as the food, detergent, pharmaceutical, textile, and pulp and paper sectors. A novel aqueous two-phase system composed of surfactant and xylitol was employed for the first time to purify lipase from Cucurbita moschata. The influence of different parameters such as type and concentration of surfactants, and the composition of the surfactant/xylitol mixtures on the partitioning behavior and recovery of lipase was investigated. Moreover, the effect of system pH and crude load on the degree of purification and yield of the purified lipase were studied. The results indicated that the lipase was partitioned into the top surfactant rich phase while the impurities partitioned into the bottom xylitol-rich phase using an aqueous two phase system composed of 24% (w/w) Triton X-100 and 20% (w/w) xylitol, at 56.2% of tie line length (TLL), (TTL is one of the important parameters in this study and it is determined from a bimodal curve in which the tie-line connects two nodes on the bimodal, that represent concentration of phase components in the top and bottom phases) and a crude load of 25% (w/w) at pH 8.0. Recovery and recycling of components was also measured in each successive step process. The enzyme was successfully recovered by the proposed method with a high purification factor of 16.4 and yield of 97.4% while over 97% of the phase components were also recovered and recycled. This study demonstrated that the proposed novel aqueous two phase system method is more efficient and economical than the traditional aqueous two phase system method for the purification and recovery of the valuable enzyme lipase.

  15. A Novel Aqueous Two Phase System Composed of Surfactant and Xylitol for the Purification of Lipase from Pumpkin (Cucurbita moschata Seeds and Recycling of Phase Components

    Directory of Open Access Journals (Sweden)

    Mehrnoush Amid

    2015-06-01

    Full Text Available Lipase is one of the more important enzymes used in various industries such as the food, detergent, pharmaceutical, textile, and pulp and paper sectors. A novel aqueous two-phase system composed of surfactant and xylitol was employed for the first time to purify lipase from Cucurbita moschata. The influence of different parameters such as type and concentration of surfactants, and the composition of the surfactant/xylitol mixtures on the partitioning behavior and recovery of lipase was investigated. Moreover, the effect of system pH and crude load on the degree of purification and yield of the purified lipase were studied. The results indicated that the lipase was partitioned into the top surfactant rich phase while the impurities partitioned into the bottom xylitol-rich phase using an aqueous two phase system composed of 24% (w/w Triton X-100 and 20% (w/w xylitol, at 56.2% of tie line length (TLL, (TTL is one of the important parameters in this study and it is determined from a bimodal curve in which the tie-line connects two nodes on the bimodal, that represent concentration of phase components in the top and bottom phases and a crude load of 25% (w/w at pH 8.0. Recovery and recycling of components was also measured in each successive step process. The enzyme was successfully recovered by the proposed method with a high purification factor of 16.4 and yield of 97.4% while over 97% of the phase components were also recovered and recycled. This study demonstrated that the proposed novel aqueous two phase system method is more efficient and economical than the traditional aqueous two phase system method for the purification and recovery of the valuable enzyme lipase.

  16. Technical and economic aspects of purification strategies to minimise discharge water from companies with closed soilless cultivation systems

    NARCIS (Netherlands)

    Os, E.A. van; Bruins, M.; Beerling, E.; Jurgens, R.; Appelman, W.; Enthoven, N.

    2014-01-01

    The aim of the research project was to achieve closure by two complementary means: 1) maximising reuse of the nutrient solution by solving problems in recirculation that leads to discharge, and 2) purification of the left over discharged water. In this paper the technical and economic aspects of pur

  17. Technical and economic aspects of purification strategies to minimise discharge water from companies with closed soilless cultivation systems

    NARCIS (Netherlands)

    Os, van E.A.; Bruins, M.A.; Beerling, E.A.M.; Jurgens, R.; Appelman, W.; Enthoven, N.

    2014-01-01

    The aim of the research project was to achieve closure by two complementary means: 1) maximising reuse of the nutrient solution by solving problems in recircula-tion that leads to discharge, and 2) purification of the left over discharged water. In this paper the technical and economic aspects of

  18. Assessment of internal contamination problems associated with bioregenerative air/water purification systems

    Science.gov (United States)

    Johnson, Anne H.; Bounds, B. Keith; Gardner, Warren

    1990-01-01

    The emphasis is to characterize the mechanisms of bioregenerative revitalization of air and water as well as to assess the possible risks associated with such a system in a closed environment. Marsh and aquatic plants are utilized for purposes of wastewater treatment as well as possible desalinization and demineralization. Foliage plants are also being screened for their ability to remove toxic organics from ambient air. Preliminary test results indicate that treated wastewater is typically of potable quality with numbers of pathogens such as Salmonella and Shigella significantly reduced by the artificial marsh system. Microbiological analyses of ambient air indicate the presence of bacilli as well as thermophilic actinomycetes.

  19. Standardization of water purification in the central dialysis fluid delivery system: validation and parametric method.

    Science.gov (United States)

    Tomo, Tadashi; Shinoda, Tosiho

    2009-01-01

    The central dialysis fluid delivery system (CDDS) has been mainly used for hemodialysis therapy in Japan. Validation and a parametric method are necessary for the quality control of dialysis fluid in CDDS. Validation is a concept for the assurance of system compatibility and product quality, and is defined as follows: the manufacturing and quality control methods including the system design and equipment of the manufacturing facility, manufacturing procedure and processes. Confirmed results must be kept within acceptable limits and they must be documented in a record. Important parameters for validating CDDS include: (1) setting the sterilized area; (2) decision of sterilization level; (3) confirmation of the maximum bio-burden; (4) performance of endotoxin retentive filter and reverse osmosis (RO) module, and (5) checkpoints of purity of dialysis water in the system. Taking the concept of validation and a parametric method in the management of CDDS into consideration enables the supply the purified dialysis fluid or the online prepared substitution fluid that meet the 2008 standards of the Japanese Society for Dialysis Therapy.

  20. [Potential of nitrification and denitrification in water purification system with hydroponic bio-filter method].

    Science.gov (United States)

    Li, Xian-ing; Lu, Xi-wu; Song, Hai-liang; Osamu, Nishimura; Yuhei, Inamori

    2005-03-01

    The potential of nitrification and denitrification of sediment and the density of ammonium-oxidizing bacteria and nitrite-oxidizing bacteria in sediment in water quality purifying system with hydroponic bio-filter method (HBFM) were measured. The variation of nitrification and denitrification potential of the sediment along the stream way was quantitatively studied. The results show that among the sediments from front, middle and retral part of the stream way, the sediment from middle part reached a maximum nitrification potential . nitrification potential of 4.76 x 10(-6) g/(g x h), while the sediment from front part reached a maximum denitrification potential of 8 .1 x 10(-7) g/(g x h). The distribution of nitrification potential accords with the ammonium-oxidizing bacteria density. The key for improving nitrogen removal efficiency of HBFM system consists in changing nitrification & denitrification region distributing and accordingly enhances denitrification process.

  1. Use of Barley for the Purification of Aquaculture Wastewater in a Hydroponics System

    Directory of Open Access Journals (Sweden)

    A. M. Snow

    2008-01-01

    Full Text Available Barley was examined for its ability to remove nutrients from aquaculture wastewater. The effects of seed sterilization using ethanol and bleach and seed density on germination and plant growth were investigated. Surface sterilization of barley seeds had a negative impact on germination. Increasing the ethanol concentration and/or the bleach concentration reduced the germination percentage. Barley seeds were first germinated in water in the hydroponics system. The seedlings then received wastewater from an aquaculture system stocked with Arctic charr. During the experiment, the crops grew rapidly and fairly uniformly and showed no signs of mineral deficiency or disease. The average crop height at harvest was 25.5 cm and the yield varied from 25 to 59 t haˉ1, depending on the seed density. The hydroponically grown barley was able to significantly reduce the pollution load of the aquaculture wastewater. The TS, COD, NH4+-N, NO2--N, NO3--N, and PO43--P reductions ranged from 52.7 to 60.5%, from 72.9 to 83.1%, from 76.0 to 76.0%, from 97.6 to 99.2%, from 76.9 to 81.6% and from 87.1 to 95.1%, respectively. However, the effluent produced from the hydroponics system had slightly higher levels of TS (420-485 mg Lˉ1 than the 480 mg Lˉ1 recommended for aquatic animals. A sedimentation/filtration unit should be added to the hydroponics system.

  2. Nanolipoprotein particles and related methods and systems for protein capture, solubilization, and/or purification

    Energy Technology Data Exchange (ETDEWEB)

    Chromy, Brett A.; Henderson, Paul; Hoeprich, Jr, Paul D.

    2016-10-04

    Provided herein are methods and systems for assembling, solubilizing and/or purifying a membrane associated protein in a nanolipoprotein particle, which comprise a temperature transition cycle performed in presence of a detergent, wherein during the temperature transition cycle the nanolipoprotein components are brought to a temperature above and below the gel to liquid crystalling transition temperature of the membrane forming lipid of the nanolipoprotein particle.

  3. Design approaches for a cycling adsorbent/photocatalyst system for indoor air purification: formaldehyde example.

    Science.gov (United States)

    Chin, Paul; Ollis, David F

    2008-04-01

    A kinetic model for a cycling adsorbent/photocatalyst combination for formaldehyde removal in indoor air (Chin et al. J. Catalysis 2006, 237, 29-37) was previously developed in our lab, demonstrating agreement with lab-scale batch operation data of other researchers (Shiraishi et al. Chem. Engineer. Sci. 2003, 58, 929-934). Model parameters evaluated included adsorption equilibrium and rate constants for the adsorbent (activated carbon) honeycomb rotor, and catalytic rate constant for pseudo-first-order formaldehyde destruction in the titanium dioxide photoreactor. This paper explores design consequences for this novel system. In particular, the batch parameter values are used to model both adsorbent and photocatalyst behavior for continuous operation in typical residential home challenges. Design variables, including realistic make-up air fraction, adsorbent honeycomb rotation speed, and formaldehyde source emission rate, are considered to evaluate the ability of the system to achieve World Health Organization pollutant guidelines. In all circumstances, the size of the required rotating adsorbent bed and photoreactor for single-stage operation and the resultant formaldehyde concentration in the home are calculated. The ability of how well such a system might be accommodated within the typical dimensions of commercial ventilation ducts is also considered.

  4. Applications of Water Fog Purification System in the Flare System%水雾净化系统在火炬回收系统中的应用

    Institute of Scientific and Technical Information of China (English)

    白丽影; 熊梦林

    2012-01-01

    The gas from refineries contains hydrogen sulfide, fine coke, catalyst fines, rusty stain, which makes equipments and pipelines corrode, leak, block and destroy to seriously influence safe operation of the flare system. In this paper, the working principle of water fog purification system was introduced as well as its application in the flare system, and some suggestions were put forward to solve the problems in the operation, which can ensure the best work condition of the system.%炼油装置产生的瓦斯气中含有的硫化氢、焦粉、催化剂粉末、锈渣等成分,极易造成设备、管线腐蚀泄漏、堵塞损坏,给火炬回收系统的安全运行带来严重影响.针对某炼油厂增上的水雾净化系统的工作原理和在火炬回收系统的应用情况进行了介绍,针对运行中出现的问题提出了整改建议,以确保实现系统最佳运行工况.

  5. Phase system selection with fractional factorial design for purification of recombinant cyanovirin-N from a hydroponic culture medium using centrifugal partition chromatography.

    Science.gov (United States)

    Grudzień, Łukasz; Madeira, Luisa; Fisher, Derek; Ma, Julian; Garrard, Ian

    2013-04-12

    Centrifugal partition chromatography (CPC) with an aqueous two-phase system (ATPS) was used to purify recombinant cyanovirin-N (CV-N) from other proteins which were co-secreted into a hydroponic plant medium in a rhizosecretion process. To achieve satisfactory protein concentration, the purification was preceded by ultrafiltration performed on a 5 kDa filter. ATPS, because of their gentle nature, were selected as the phase system for CPC. A systematic phase system selection was applied. This involved studying the effect of seven parameters of ATPS: polymer type, salt type, the polymer and salt concentration, the polymer molecular weight, pH, and presence of two additional salts; NaCl and NaClO4, which all together gave 320 combinations. design of experiment (DoE) software allowed the reduction of this number to 46. Having tested partitioning of cyanovirin-N and impurities in 46 ATPS, the three best potential phase systems generated by the programme were then tested on the CPC. Out of these three, 13/13% PEG4000 sodium phosphate, pH 3.0, proved to be most effective phase system in the purification of cyanovirin-N, judged by ELISA and SDS-PAGE analysis, as it eliminated most of the impurities from the final cyanovirin-N preparation.

  6. Liquid Argon Dielectric Breakdown Studies with the MicroBooNE Purification System

    Energy Technology Data Exchange (ETDEWEB)

    Acciarri, R. [Fermilab; Carls, B. [Fermilab; James, C. [Fermilab; Johnson, B. [Fermilab; Jostlein, H. [Fermilab; Lockwitz, S. [Fermilab; Lundberg, B. [Fermilab; Raaf, J. L. [Fermilab; Rameika, R. [Fermilab; Rebel, B. [Fermilab; Zeller, G. P. [Fermilab; Zuckerbrot, M. [Fermilab

    2014-11-04

    The proliferation of liquid argon time projection chamber detectors makes the characterization of the dielectric properties of liquid argon a critical task. To improve understanding of these properties, a systematic study of the breakdown electric field in liquid argon was conducted using a dedicated cryostat connected to the MicroBooNE cryogenic system at Fermilab. An electrode sphere-plate geometry was implemented using spheres with diameters of 1.3 mm, 5.0 mm, and 76 mm. The MicroBooNE cryogenic system allowed measurements to be taken at a variety of electronegative contamination levels ranging from a few parts-per-million to tens of parts-per-trillion. The cathode-anode distance was varied from 0.1 mm to 2.5 cm. The results demonstrate a geometric dependence of the electric field strength at breakdown. This study is the first time that the dependence of the breakdown field on stressed cathode area has been shown for liquid argon.

  7. Liquid Argon Dielectric Breakdown Studies with the MicroBooNE Purification System

    CERN Document Server

    Acciarri, R; James, C; Johnson, B; Jostlein, H; Lockwitz, S; Lundberg, B; Raaf, J L; Rameika, R; Rebel, B; Zeller, G P; Zuckerbrot, M

    2014-01-01

    The proliferation of liquid argon time projection chamber detectors makes the characterization of the dielectric properties of liquid argon a critical task. To improve understanding of these properties, a systematic study of the breakdown electric field in liquid argon was conducted using a dedicated cryostat connected to the MicroBooNE cryogenic system at Fermilab. An electrode sphere-plate geometry was implemented using spheres with diameters of 1.3 mm, 5.0 mm, and 76 mm. The MicroBooNE cryogenic system allowed measurements to be taken at a variety of electronegative contamination levels ranging from a few parts-per-million to tens of parts-per- trillion. The cathode-anode distance was varied from 0.1 mm to 2.5 cm. The results demonstrate a geometric dependence of the electric field strength at breakdown. This study is the first time that the dependence of the breakdown field on stressed cathode area has been shown for liquid argon.

  8. Liquid argon dielectric breakdown studies with the MicroBooNE purification system

    Energy Technology Data Exchange (ETDEWEB)

    Acciarri, R.; Carls, B.; James, C.; Johnson, B.; Jostlein, H.; Lockwitz, S.; Lundberg, B.; Raaf, J. L.; Rameika, R.; Rebel, B.; Zeller, G. P.; Zuckerbrot, M.

    2014-11-01

    The proliferation of liquid argon time projection chamber detectors makes the characterization of the dielectric properties of liquid argon a critical task. To improve understanding of these properties, a systematic study of the breakdown electric field in liquid argon was conducted using a dedicated cryostat connected to the MicroBooNE cryogenic system at Fermilab. An electrode sphere-plate geometry was implemented using spheres with diameters of 1.3 mm, 5.0 mm, and 76 mm. The MicroBooNE cryogenic system allowed measurements to be taken at a variety of electronegative contamination levels ranging from a few parts-per-million to tens of parts-per-trillion. The cathode-anode distance was varied from 0.1 mm to 2.5 cm. The results demonstrate a geometric dependence of the electric field strength at breakdown. This study is the first time that the dependence of the breakdown field on stressed cathode area has been shown for liquid argon.

  9. Diseño y construcción de una bomba manual de émbolo para cisternas de aguas pluviales y pozos someros

    OpenAIRE

    Polo Castaño, Christian Daniel

    2014-01-01

    El objetivo de este proyecto es hacer un estudio sobre las bombas manuales de émbolo como tecnología social, analizando su implicación en el “Programa Um Milhão de Cisternas” – Programa un millón de cisternas - (P1MC) que se desarrolla en el semiárido brasileño basado en la recogida de agua de lluvia, ya que es una de las formas más sencillas y de bajo costo para paliar el déficit de agua en la región. El caso de estudio serán las bombas Carcará I y II cuya fabricación se hace con elemen...

  10. Diseño de una bomba/motor hidraulica de pistones para impulsar aceite a presion o producir movimiento de rotacion a partir de éste

    OpenAIRE

    GIMÉNEZ MARÍN, HÉCTOR

    2016-01-01

    [ES] El trabajo incluye el diseño de las piezas y el conjunto mecánico de la de la bomba/motor mediante piezas que incluyan la disposicion radial de cilindros-piston con una funcionamiento reversible. El trabajo comprende modelado 3D de los componentes mecánicos, generación del prototipo virtual, simulación del funcionamiento, Análisis de esfuerzos y fatiga de uno de sus elementos, y generación de documentación y planos de detalle. Giménez Marín, H. (2016). Diseño de una bomba/motor hi...

  11. Avaliação do redimento de bombas hidráulicas de engrenagens externas através de medição de temperatura

    OpenAIRE

    Dalla Lana, Eduardo

    2005-01-01

    Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro Tecnológico. Programa de Pós-Graduação em Engenharia Mecânica. Este trabalho trata do estudo teórico experimental sobre a determinação dos níveis de rendimento de uma bomba hidráulica de engrenagens externas, baseado na análise da temperatura do fluido hidráulico que circula pela mesma. Com base nas equações da mecânica dos fluidos e da termodinâmica básica, modela-se o comportamento da bomba de engrenagens em regime p...

  12. Avaliação do redimento de bombas hidráulicas de engrenagens externas através de medição de temperatura

    OpenAIRE

    2005-01-01

    Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro Tecnológico. Programa de Pós-Graduação em Engenharia Mecânica. Este trabalho trata do estudo teórico experimental sobre a determinação dos níveis de rendimento de uma bomba hidráulica de engrenagens externas, baseado na análise da temperatura do fluido hidráulico que circula pela mesma. Com base nas equações da mecânica dos fluidos e da termodinâmica básica, modela-se o comportamento da bomba de engrenagens em regime p...

  13. Countercurrent chromatographic separation and purification of various ribonucleases using a small-scale cross-axis coil planet centrifuge with aqueous-aqueous polymer phase systems.

    Science.gov (United States)

    Shinomiya, Kazufusa; Kobayashi, Hiroko; Motoyoshi, Naomi; Inokuchi, Norio; Nakagomi, Kazuya; Ito, Yoichiro

    2009-04-01

    Countercurrent chromatographic (CCC) separation and purification of various ribonucleases (RNases) was performed using the small-scale cross-axis coil planet centrifuge (X-axis CPC) with aqueous-aqueous polymer phase systems. RNases B and A were well resolved from each other with an aqueous-aqueous polymer phase system composed of 12.5% (w/w) polyethylene glycol (PEG) 1000 and 12.5% (w/w) dibasic potassium phosphate (pH 9.2) as the mobile lower phase. The commercial RNase A samples obtained from three different companies were also highly purified using the 16.0% (w/w) PEG 1000-6.3% (w/w) dibasic potassium phosphate-6.3% (w/w) monobasic potassium phosphate system (pH 6.6) using the upper phase as the mobile phase. Recombinant RNase Po(1), an RNase T(1) family enzyme, was further successfully separated from the crude extract using the same solvent system with the lower phase used as the mobile phase. The RNase activities were well preserved during the CCC separation. The overall results demonstrate that the small-scale X-axis CPC is useful for a simple and rapid purification of various RNases in a preparative-scale.

  14. Centrifugal LabTube platform for fully automated DNA purification and LAMP amplification based on an integrated, low-cost heating system.

    Science.gov (United States)

    Hoehl, Melanie M; Weißert, Michael; Dannenberg, Arne; Nesch, Thomas; Paust, Nils; von Stetten, Felix; Zengerle, Roland; Slocum, Alexander H; Steigert, Juergen

    2014-06-01

    This paper introduces a disposable battery-driven heating system for loop-mediated isothermal DNA amplification (LAMP) inside a centrifugally-driven DNA purification platform (LabTube). We demonstrate LabTube-based fully automated DNA purification of as low as 100 cell-equivalents of verotoxin-producing Escherichia coli (VTEC) in water, milk and apple juice in a laboratory centrifuge, followed by integrated and automated LAMP amplification with a reduction of hands-on time from 45 to 1 min. The heating system consists of two parallel SMD thick film resistors and a NTC as heating and temperature sensing elements. They are driven by a 3 V battery and controlled by a microcontroller. The LAMP reagents are stored in the elution chamber and the amplification starts immediately after the eluate is purged into the chamber. The LabTube, including a microcontroller-based heating system, demonstrates contamination-free and automated sample-to-answer nucleic acid testing within a laboratory centrifuge. The heating system can be easily parallelized within one LabTube and it is deployable for a variety of heating and electrical applications.

  15. Purification and concentration of alphavirus.

    Science.gov (United States)

    Lundstrom, Kenneth

    2012-07-01

    The alphaviruses Semliki Forest virus and Sindbis virus have been used frequently as expression vectors in vitro and in vivo. Usually, these systems consist of replication-deficient vectors that require a helper vector for packaging of recombinant particles. Replication-proficient vectors have also been engineered. Alphaviral vectors can be used as nucleic-acid-based vectors (DNA and RNA) or infectious particles. High-titer viral production is achieved in alphaviruses facilitates studies in mammalian and nonmammalian cell lines, primary cells in culture, and in vivo. The strong preference for expression in neuronal cells has made alphaviruses particularly useful in neurobiological studies. Unfortunately, their strong cytotoxic effect on host cells, relatively short-term transient expression patterns, and the reasonably high cost of viral production remain drawbacks. However, novel mutant alphaviruses have showed reduced cytotoxicity and prolonged expression. Membrane proteins (which are generally difficult to express at high levels in recombinant systems) have generated high yields and facilitate applications in structural biology. Alphaviruses have also been applied in vaccine development and gene therapy. Generally, purification or concentration of alphaviruses is not necessary. However, for instance, the medium derived from baby hamster kidney cells is toxic to primary neurons in culture. Including a purification step substantially improves the survival of the transduced neurons. Viral concentration and purification may also be advantageous for in vivo studies in animal models and are mandatory for clinical applications. This protocol describes three methods for purification and concentration of alphavirus.

  16. Air purification from TCE and PCE contamination in a hybrid bioreactors and biofilter integrated system.

    Science.gov (United States)

    Tabernacka, Agnieszka; Zborowska, Ewa; Lebkowska, Maria; Borawski, Maciej

    2014-01-15

    A two-stage waste air treatment system, consisting of hybrid bioreactors (modified bioscrubbers) and a biofilter, was used to treat waste air containing chlorinated ethenes - trichloroethylene (TCE) and tetrachloroethylene (PCE). The bioreactor was operated with loadings in the range 0.46-5.50gm(-3)h(-1) for TCE and 2.16-9.02gm(-3)h(-1) for PCE. The biofilter loadings were in the range 0.1-0.97gm(-3)h(-1) for TCE and 0.2-2.12gm(-3)h(-1) for PCE. Under low pollutant loadings, the efficiency of TCE elimination was 23-25% in the bioreactor and 54-70% in the biofilter. The efficiency of PCE elimination was 44-60% in the bioreactor and 50-75% in the biofilter. The best results for the bioreactor were observed one week after the pollutant loading was increased. However, the process did not stabilize. In the next seven days contaminant removal efficiency, enzymatic activity and biomass content were all diminished.

  17. Purification and in situ immobilization of papain with aqueous two-phase system.

    Science.gov (United States)

    Li, Mingliang; Su, Erzheng; You, Pengyong; Gong, Xiangyu; Sun, Ming; Xu, Diansheng; Wei, Dongzhi

    2010-12-13

    Papain was purified from spray-dried Carica papaya latex using aqueous two-phase system (ATPS). Then it was recovered from PEG phase by in situ immobilization or preparing cross-linked enzyme aggregates (CLEAs). The Plackett-Burman design and the central composite design (CCD) together with the response surface methodology (RSM) were used to optimize the APTS processes. The highly purified papain (96-100%) was achieved under the optimized conditions: 40% (w/w) 15 mg/ml enzyme solution, 14.33-17.65% (w/w) PEG 6000, 14.27-14.42% (w/w) NaH2PO4/K2HPO4 and pH 5.77-6.30 at 20°C. An in situ enzyme immobilization approach, carried out by directly dispersing aminated supports and chitosan beads into the PEG phase, was investigated to recover papain, in which a high immobilization yield (>90%) and activity recovery (>40%) was obtained. Moreover, CLEAs were successfully used in recovering papain from PEG phase with a hydrolytic activity hundreds times higher than the carrier-bound immobilized papain.

  18. Phospholipid polymer-based antibody immobilization for cell rolling surfaces in stem cell purification system.

    Science.gov (United States)

    Mahara, Atsushi; Chen, Hao; Ishihara, Kazuhiko; Yamaoka, Tetsuji

    2014-01-01

    We previously developed an antibody-conjugated cell rolling column that successfully separates stem cell subpopulations depending on the cell surface marker density, but a large amount of the injected cells were retained in the column because of non-specific interactions. In this study, an amphiphilic copolymer, poly[2-methacryloyloxyethyl phosphorylcholine (MPC)-co-n-butyl methacrylate (nBMA)-co-N-vinyl formamide (NVf)], with phospholipid polar side groups was designed as a novel antibody-immobilizing modifier. The formamide groups in NVf units were converted to active maleimide groups. A plastic flow microfluidic chamber was coated with the copolymers, and a reduced anti-CD90 antibody was immobilized. The adipose tissue-derived stem cells isolated from the rat were injected into the flow chamber, and their rolling behavior was observed under a microscope with a high-speed camera. Non-specific cell adhesion was reduced strongly by means of this immobilization method because of the MPC unit, resulting in a high percentage of rolling cells. These results demonstrate that a surface coated with phospholipid polar groups can be used in an effective stem cell separation system based on the cell rolling process.

  19. Expression and Purification of C-Peptide Containing Insulin Using Pichia pastoris Expression System

    Directory of Open Access Journals (Sweden)

    Mohammed N. Baeshen

    2016-01-01

    Full Text Available Increase in the incidence of Insulin Dependent Diabetes Mellitus (IDDM among people from developed and developing countries has created a large global market for insulin. Moreover, exploration of new methods for insulin delivery including oral or inhalation route which require very high doses would further increase the demand of cost-effective recombinant insulin. Various bacterial and yeast strains have been optimized to overproduce important biopharmaceuticals. One of the approaches we have taken is the production of recombinant human insulin along with C-peptide in yeast Pichia pastoris. We procured a cDNA clone of insulin from Origene Inc., USA. Insulin cDNA was PCR amplified and cloned into yeast vector pPICZ-α. Cloned insulin cDNA was confirmed by restriction analysis and DNA sequencing. pPICZ-α-insulin clone was transformed into Pichia pastoris SuperMan5 strain. Several Zeocin resistant clones were obtained and integration of insulin cDNA in Pichia genome was confirmed by PCR using insulin specific primers. Expression of insulin in Pichia clones was confirmed by ELISA, SDS-PAGE, and Western blot analysis. In vivo efficacy studies in streptozotocin induced diabetic mice confirmed the activity of recombinant insulin. In conclusion, a biologically active human proinsulin along with C-peptide was expressed at high level using Pichia pastoris expression system.

  20. Energy saving in hotels by means of heat pumps application; Ahorro de energia en hoteles mediante la aplicacion de bombas de calor

    Energy Technology Data Exchange (ETDEWEB)

    Romero Paredes R, Hernando; Ambriz G, Juan Jose [Universidad Autonoma Metropolitana, Unidad Iztapalapa (Mexico)

    2005-07-15

    The thermal energy is presented as an alternate saving technology for utilities by means of the acute implementation of heat pumps. The objective is to demonstrate the technical and economical feasibility of the application of commercial heat pumps to installations where the handling of opposite temperatures for the conditioning of a space and/or of a fluid is required. In this article it is described the functioning of a heat pump, its different operation principles or types as well as the large diversity of applications they can have in industry, utilities and residential buildings. As an application example the substitution of an electric boiler by a heat pump is presented in a hotel installation and its differences in performance regarding the environmental conditioning of a nearby area and the heating of swimming pool water are analyzed. [Spanish] Se presenta la energia termica como una tecnologia de ahorro alterna para empresas de servicios valiendose de la implementacion agudizada de bombas de calor. El objetivo es demostrar la factibilidad tecnica y economica de la aplicacion de bombas de calor comerciales a instalaciones donde se requiere la manipulacion de temperaturas opuestas para el acondicionamiento de un espacio y/o un fluido. Se describe el funcionamiento de una bomba de calor, sus diferentes principios de operacion o tipos, asi como la gran diversidad de aplicaciones que pueden tener en la industria, empresas de servicios y edificios residenciales. Como ejemplo de aplicacion se presenta la sustitucion de una caldera electrica por una bomba de calor en una instalacion hotelera y sus diferencias de rendimiento en el calentamiento de agua de alberca y el acondicionamiento ambiental de un area cercana.

  1. Digital Speed Control System Design for Air Purification Equipment%空气净化装置数字化调速控制系统设计

    Institute of Scientific and Technical Information of China (English)

    石宝松; 张伟; 刘剑; 韩振伟

    2014-01-01

    地平式激光跟踪发射系统为保证其光路洁净,须进行正压防尘处理。针对外购空气净化设备仅能采用手动风速调节,不能实现计算机远程控制的不足,研发了空气净化设备数字化调速控制系统,以实现对空气净化装置的远程控制与状态监测。本设计采用光电隔离单相交流调压模块实现对风机的驱动,采用霍尔电流传感器实现对风机的电流测量,并采用内部集成A/D转换器和D/A转换器的单片机通过RS422总线接收控制指令,完成对调压模块的输出功率控制和风机电流的实时采集。本系统设计简洁、控制方便、运行可靠、易于维护,实现了设备管理计算机对空气净化设备风速的远程控制。%Horizon emitting laser tracking system to ensure its optical path cleaning, shall be positive dust treatment. For the lack of air purification equipment purchased only with manual speed adjustment and the remote control of a computer can not be achieved, we have developed an air purification equipment digital speed control system to achieve the remote control and status monitoring of air purification device. The design uses a optically isolated single-phase AC voltage regulator module for fan drive,uses a Hall current sensors to achieve the fan current measurement,and uses a microcontroller, which integrates a A/D converter and a D/A converter, to receive the control command via the RS422 bus, to complete the output power control of voltage regulator module, and to realize the real-time collection of fan’ s current. The system is designed to be simple, easy to control, reliable to operate and easy to maintain and realizes the remote control of a device management computer on the wind speed of air purification equipment.

  2. Inverse steptoes in Las Bombas volcano, as an evidence of explosive volcanism in a solidified lava flow field. Southern Mendoza-Argentina

    Science.gov (United States)

    Risso, Corina; Prezzi, Claudia; Orgeira, María Julia; Nullo, Francisco; Margonari, Liliana; Németh, Karoly

    2015-11-01

    Here we describe the unusual genesis of steptoes in Las Bombas volcano- Llancanelo Volcanic Field (LVF) (Pliocene - Quaternary), Mendoza, Argentina. Typically, a steptoe forms when a lava flow envelops a hill, creating a well-defined stratigraphic relationship between the older hill and the younger lava flow. In the Llancanelo Volcanic Field, we find steptoes formed with an apparent normal stratigraphic relationship but an inverse age-relationship. Eroded remnants of scoria cones occur in ;circular depressions; in the lava field. To express the inverse age-relationship between flow fields and depression-filled cones here we define this landforms as inverse steptoes. Magnetometric analysis supports this inverse age relationship, indicating reverse dipolar magnetic anomalies in the lava field and normal dipolar magnetization in the scoria cones (e.g. La Bombas). Negative Bouguer anomalies calculated for Las Bombas further support the interpretation that the scoria cones formed by secondary fracturing on already solidified basaltic lava flows. Advanced erosion and mass movements in the inner edge of the depressions created a perfectly excavated circular depression enhancing the ;crater-like; architecture of the preserved landforms. Given the unusual genesis of the steptoes in LVF, we prefer the term inverse steptoe for these landforms. The term steptoe is a geomorphological name that has genetic implications, indicating an older hill and a younger lava flow. Here the relationship is reversed.

  3. Bioinspired Materials for Water Purification

    Directory of Open Access Journals (Sweden)

    Alfredo Gonzalez-Perez

    2016-06-01

    Full Text Available Water scarcity issues associated with inadequate access to clean water and sanitation is a ubiquitous problem occurring globally. Addressing future challenges will require a combination of new technological development in water purification and environmental remediation technology with suitable conservation policies. In this scenario, new bioinspired materials will play a pivotal role in the development of more efficient and environmentally friendly solutions. The role of amphiphilic self-assembly on the fabrication of new biomimetic membranes for membrane separation like reverse osmosis is emphasized. Mesoporous support materials for semiconductor growth in the photocatalytic degradation of pollutants and new carriers for immobilization of bacteria in bioreactors are used in the removal and processing of different kind of water pollutants like heavy metals. Obstacles to improve and optimize the fabrication as well as a better understanding of their performance in small-scale and pilot purification systems need to be addressed. However, it is expected that these new biomimetic materials will find their way into the current water purification technologies to improve their purification/removal performance in a cost-effective and environmentally friendly way.

  4. Effects of indoor air purification by an air cleaning system (Koala technology) on semen parameters in male factor infertility: results of a pilot study.

    Science.gov (United States)

    Paradisi, R; Vanella, S; Barzanti, R; Cani, C; Battaglia, C; Seracchioli, R; Venturoli, S

    2009-06-01

    A number of studies indicated a clear decline in semen quality in the past 30-50 years and there is accumulating evidence that this decline might result from exposure to high levels of air pollution. To examine the impact of environment on male reproductive ability, we undertook for the first time a pilot study on semen quality of infertile men exposed to purification of indoor air. Ten subjects with a history of unexplained male infertility and poor semen quality were exposed for at least 1 year to a cleaning indoor air system (Koala technology). The key feature of this air purifier is the unique innovative multiple filtering system. The treatment of total purification of indoor air showed neither improvements in semen parameters nor variation in reproductive hormones (P = N.S.), but induced an evident increase (P indoor air does not seem enough to improve semen quality, although the increase in leucocytic concentrations could indicate an activation of the role of immunosurveillance in a purified indoor air environment.

  5. Metodología para el diseño de bomba de pistones axiales para máquinas pulverizadoras

    Directory of Open Access Journals (Sweden)

    José Antonio Gómez Bauzá

    2010-01-01

    Full Text Available Este trabajo tiene por objetivo desarrollar un estudio hidromecánico integrador, orientado al diseño de bombas de pistones axiales de máquinas pulverizadoras para la agricultura, basado no solo en estudios anteriores sino en la experiencia acumulada durante los años de trabajo en este tema, dado que la información existente está dirigida fundamentalmente a la selección y explotación de este componente, no sucediendo así para el diseño, y esto dificulta en gran medida el desarrollo de estos equipos en países como Cuba que no cuentan con una tradición y experiencia en este campo, lo que demuestra la importancia del tema para el desarrollo de estos equipos en el país. Para ello se propone una metodología de cálculo específicamente orientada al diseño de estos equipos, y además se realiza un estudio estadístico basado en los datos recopilados de más de cien bombas de producción extranjera de diecinueve firmas de reconocido prestigio internacional que permiten analizar aspectos como: velocidad máxima real de desplazamiento del pistón, caudal y presión de trabajo para estas bombas, respondiendo a las nuevas tendencias internacionales. El aporte teórico de la investigación radica en que este estudio constituye una guía metodológica integradora para el diseño de este tipo de bombas y como aporte práctico la fabricación de estas bombas con la consiguiente sustitución de importaciones y la puesta en marcha del parque de máquinas pulverizadoras, fundamentalmente de producción nacional, que en la actualidad requieren de este componente.

  6. Bloqueio contínuo do plexo lombar via posterior bilateral com bomba de infusão descartável: relato de caso

    Directory of Open Access Journals (Sweden)

    Luiz Eduardo Imbelloni

    2011-04-01

    Full Text Available JUSTIFICATIVA E OBJETIVOS: O número de artroplastia total de quadril (ATQ bilateral tem aumentado a cada ano. Analgesia pós-operatória pela infusão contínua perineural com anestésico local tem se mostrado favorável quando comparada com analgesia sistêmica. O uso de bombas elastoméricas tem aumentado a satisfação do paciente quando em comparação com os modelos eletrônicos. O objetivo deste relato foi descrever um caso de analgesia contínua bilateral do plexo lombar via posterior, com infusão contínua através de bomba elastomérica, em paciente submetido à uma artroplastia bilateral de quadril. RELATO DO CASO: Paciente feminina, 46 anos, 65 kg, 162 cm, com artrite reumatoide e hipertensão arterial, estado físico ASA II, escalada para ser submetida a ATQ bilateral em um único estágio. Uso de corticosteroide por 13 anos. Hemoglobina = 10,1 g.dL-1, hematócrito = 32,7%. Monitoração de rotina. Raquianestesia com 15 mg de bupivacaína 0,5% isobárica. Anestesia geral com propofol (PFS e remifentanil e intubação sem bloqueadores neuromusculares. ATQ direita e no final, bloqueio plexo lombar com estimulador e conjunto agulha 150 mm e injeção de 20 mL bupivacaína 0,2% e passagem de cateter. ATQ esquerda e, no final, mesmo procedimento. Estudado dispersão do anestésico e contraste. Instalado bomba elastomérica com bupivacaína 0,1% (400 mL em velocidade de 14 mL.h-1. Transferida para Unidade de Cuidados Intensivos (UCI. Vinte e quatro horas após, nova bomba com a mesma solução. Nenhum bolus durante 50 horas. Após remoção de cateter, dor controlada por via oral com cetoprofeno e dipirona. CONCLUSÕES: O bloqueio bilateral contínuo periférico com infusão de bupivacaína a 0,1% com bombas elastoméricas é um procedimento seguro e efetivo em adultos.

  7. Evaluation of a new preparative supercritical fluid chromatography system for compound library purification: the TharSFC SFC-MS Prep-100 system.

    Science.gov (United States)

    Ebinger, Katalin; Weller, Harold N; Kiplinger, Jeffrey; Lefebvre, Paul

    2011-06-01

    Preparative HPLC-MS is often the method of choice for purification of small amounts (<100mg) of diverse new molecules, such as compound libraries for drug discovery. The method is robust, well proven, and widely applicable. In contrast, preparative supercritical fluid chromatography coupled with mass spectrometry (SFC-MS) has seen only slow acceptance for the same application--despite some potential scientific and economic advantages. One of the reasons for slow adoption of SFC-MS is the lack of well-proven, robust, and commercially available instrumentation. In early 2009, TharSFC (a Waters Company, Pittsburgh, PA) introduced a new fully integrated system for preparative SFC-MS: The SFC-MS Prep-100. We report herein an objective evaluation of the SFC-MS Prep-100, including tests for pump and autosampler performance, sample recovery, sample carryover, fraction triggering, detector/fraction collector synchronization, and overall robustness. Our results suggest that the SFC-MS Prep-100 represents a significant advance over previous generation instrumentation.

  8. Controlling Points of Air Purification System in Design and Construction Process%净化空调系统在设计施工过程中的控制要点

    Institute of Scientific and Technical Information of China (English)

    刘静

    2014-01-01

    随着高新技术的发展,对生产环境的洁净要求也越来越严格,净化空调的应用越来越广泛。该文在阐述洁净等级的划分之后,根据实际经验提出净化空调系统设计存在的问题,并分析了系统施工过程应注意的问题。%With the development of high and new technology, requirements on production environment are getting increasingly stricter, and application of air purification system is also getting increasingly wider. The classification of purification level is elaborated, the existing problems in air purification sys-tem design are presented in accordance with actual experience and some attention worth issues in construction process are also analyzed.

  9. Affinity Purification of Protein Complexes Using TAP Tags

    Science.gov (United States)

    Gerace, Erica; Moazed, Danesh

    2016-01-01

    This protocol is used for the isolation and analysis of protein complexes using the tandem affinity purification (TAP) tag system. The protocol describes the purification of a protein fused to a TAP tag comprised of two protein A domains and the calmodulin binding peptide separated by a TEV cleavage site. This is a powerful technique for rapid purification of protein complexes and the analysis of their stoichiometric composition, posttranslational modifications, structure, and functional activities. PMID:26096502

  10. ¿Qué debemos conocer de los inhibidores de bomba protones, para su uso en las unidades de dolor?

    Directory of Open Access Journals (Sweden)

    J. A. García-García

    2007-10-01

    Full Text Available Los inhibidores de la bomba de protones (IBP son fármacos útiles para el control de la patología asociada con la acidez gástrica, patología con una alta prevalencia dentro de la población general. En las Unidades de dolor tratamos pacientes con pluripatología y polimedicados, entre ellas patologías asociadas con la acidez gástrica. Así como también utilizamos fármacos como AINES, glucocorticoides, bifosfonatos... que pueden reactivar, empeorar la patología ligada a la acidez. El conocimiento de los aspectos farmacológicos de los IBP, tanto farmacocinéticos como farmacodinámicos, es necesario y preciso para poder elegir el más adecuado para nuestros pacientes con pluripatologia y polimedicados evitando las posibles interacciones farmacológicas que podrían afectar al estado de salud de nuestros pacientes.

  11. 天然气净化厂安全防火防爆系统的应用分析%Application and analysis of the safety and fire protection and explosion protection system of natural gas purification plant

    Institute of Scientific and Technical Information of China (English)

    都永昌

    2016-01-01

    本文就天然气净化厂安全防火防爆系统的应用进行分析,以促进天然气净化厂对安全工作的重视程度,提升安全管理水平。%In this paper, the application of natural gas purification plant safety fire and explosion protection system is analyzed, in order to promote the natural gas purification plant to the safety work of attention, improve the level of safety management.

  12. Efficiencies of different flue gas purification systems in waste incinerators. Experimental report; Untersuchungen zur Effizienz von unterschiedlichen Systemen zur Rauchgasreinigung bei Muellverbrennungsanlagen. Untersuchungsbericht

    Energy Technology Data Exchange (ETDEWEB)

    Berghoff, R. (comp.)

    2001-07-01

    This investigation was carried out on behalf of the Environmental Office of the state of Nordrhein-Westfalen. Apart from the current and projected exhaust purification systems, also three exemplary systems were analyzed from the ecological and economic point of view. Effects on pollutant freights were established in consideration of energy consumption and the specific cost of waste treatment for each technology. [German] Das konkrete Ziel dieser vom Landesumweltamt NRW vergebenen Untersuchung war es, neben einer Uebersicht ueber die zzt. eingesetzten und auch geplanten Abgasreinigungssysteme (RGR) bei Muellverbrennungsanlagen, die Vor- und die Nachteile von 3 beispielhaft ausgewaehlten Systemen in einem oekologischen und oekonomischen Vergleich knapp und begrenzt darstellen zu lassen. Als Ergebnis wurden die Auswirkungen der unterschiedlichen Verfahren auf die Frachten der emittierten Schadstoffe unter Beruecksichtigung der unterschiedlichen Energieverbraeuche ermittelt und dargestellt. Darueber hinaus wurden die spezifischen Muellbehandlungskosten bei den jeweiligen Gasreinigungstechniken errechnet. (orig.)

  13. Design and development of single stage purification of papain using Ionic Liquid based aqueous two phase extraction system and its Partition coefficient studies

    Directory of Open Access Journals (Sweden)

    Senthilkumar Rathnasamy

    2013-04-01

    Full Text Available As an emerging trend in bioseparation, aqueous two phase extractions based on phosponium ionic liquid have been utilized in this work to extract papain from Carica papaya fruit latex and the same wascompared with conventional aqueous two phase extraction system. Factors affecting the partition coefficient of papain such as ionic liquid concentration, pH of the extraction system and temperature have been investigated. The optimization studies show that ionic liquid concentrations and pH are majorly influencing the phaseformations and papain partitioning. It reveals the importance of electrostatic and hydrophobic interactions in the papain partitioning. Purification studies performed on Gel Filtration Chromatography shows that 96% of the papain enzyme could be extracted with the phosponium based ionic liquid in a single stage extraction. The final fraction containing papain enzyme was confirmed by SDS Page analysis.

  14. The Home Air Quality Purification System Based on Internet of Things%基于物联网的家居空气质量净化系统

    Institute of Scientific and Technical Information of China (English)

    蔡文郁; 徐建军; 王洪亮

    2016-01-01

    For the current status of air pollution and the demand for healthy indoor environment,the paper studies a type of home purification system which based on the internet of things (IoT).The system uses the ARM-Cortex-M0 core micro-controller NuMicro-M058 as the hardware platform,and RTX embedded operating system as the software platform,with three filters,a variety of purification photo catalytic disinfection,ultraviolet disinfection,etc.The air purifier also has a collection function for air quality data such as PM2.5,VOC,temperature and humidity.It also can connect through the server and mobile client applications to implement remote monitoring.%针对目前大气污染的现状和人们对健康室内环境的需求,研究并设计了一种基于物联网的家居空气质量净化系统.系统以 ARM-Cortex-M0内核微控制器 NuMicro-M058芯片为硬件核心,采用嵌入式 RTX 操作系统为软件平台,具有三层滤网、光触媒消毒、紫外线杀菌等多种净化功能,而且还具有 PM 2.5,VOC,温湿度等空气质量数据采集功能,最终通过服务器与手机端程序的编写实现了物联网化远程监控.

  15. Sewage Purification Business Process Management

    Directory of Open Access Journals (Sweden)

    Esad Ahmetagić

    2011-09-01

    Full Text Available This paper presents the current level of drainage and sewage purification facilities built in the Autonomous Province of Vojvodina, a territorial unit of the Republic of Serbia. It also points out the issues related to organized business management in companies involved in this business.The management of business processes in sewage purification involves a comprehensive cycle: business organizing process, issues of standard, investments, workforce, and information system design as factors in establishing an effective organization of business processes. The definition of gap existing between the current approach to organizing business activities and the need to establish an approach based on knowledge, information technologies, and effective business process management points to the necessity for organization redesign and standard definition in business process management. Sewage purification business process management in Vojvodina, the Republic of Serbia has been elaborated through theoretical presentation and a practical example realized by electronic ISO 9001:2008 system of quality management in public water utility company JKP "Vodokanal" Sombor.

  16. PUMPCOM: um modelo para combinação de curvas e análise do desempenho de bombas PUMPCOM: a model for pump curve combination and performance analysis

    Directory of Open Access Journals (Sweden)

    Camilo de L. T. de Andrade

    2001-12-01

    Full Text Available Um modelo de computador foi desenvolvido para combinar curvas de bombas em série e em paralelo e para determinar o desempenho da estação de bombeamento e das bombas que a compõem. Polinômios são ajustados aos dados das curvas de cada bomba e aos dados combinados da estação de bombeamento. A interpolação "spline" cúbica é utilizada para combinar curvas de bomba de capacidade diferente. Indicadores de desempenho da estação e de cada bomba, individualmente, são calculados para toda a faixa de vazão de operação do sistema. Uma interface gráfica permite ao usuário desenhar o croqui da estação de bombeamento e visualizar os dados de cada bomba e as equações ajustadas. Dados, gráficos e coeficientes de equações ajustadas podem ser impressos. Os cálculos do modelo foram verificados com planilha eletrônica.A computer model was developed to combine pump curves in series and in parallel and to determine the performance of the pump station and of individual pumps within the station. Polynomial equations are fited to individual pump curves and to the combined pump station curve. Cubic spline interpolation is employed to combine curves of pumps with different sizes. Performance indicators of the station and of each pump within the station are calculated for the entire station flow rate range. A graphical interface allows the user to draw the pump station layout and to view the discrete data entered along with the equations fit. Data entered, plots of the curves and equation coefficients can be printed out. PUMPCOM results were verified with spreadsheet calculations.

  17. 组合吸收塔在 VCM 净化系统中的应用%Application of combined absorption tower in VCM purification system

    Institute of Scientific and Technical Information of China (English)

    田长波; 邵海珑; 齐念先; 赵辉旭

    2013-01-01

    The reform of VCM purification system was performed .The falling film-water washing process was replaced by combined absorption tower process .After the reform ,the absorp-tion efficiency increased ,the water consumption and the caustic soda consumption decreased ,the device life prolonged ,and the cost was saved by 1 .464 4 million RMB ¥ per year .%  对 VCM 净化系统进行了改造,采用组合吸收塔工艺替代降膜水洗吸收工艺。改造后系统的吸收效率提高,水消耗量和烧碱用量减少,设备使用寿命延长,可节约成本146.44万元/a 。

  18. PURIFICATION AND ANALYSIS OF CARBOHYDRATE CLUSTER FROM ERYTHROPOIETIN RECOMBINANT EXPRESSION RESULT ON THE LEAVEN SYSTEM OF Pichia pastoris

    Directory of Open Access Journals (Sweden)

    Adi Santoso

    2011-04-01

    Full Text Available For clinical purposes, pure protein and identification of carbohydrate structure from erythropoietin recombinant are needed. Purification was done with His-Trap affinity chromatography method and continued with gel filtration chromatography column to get purer protein. The carbohydrate cluster from the resulting pure protein then can be recognized by using N- and O-glycosidase and can be compared to EPO recombinant from mammal cells. The result showed similarity on the declining trend of the protein molecule’s weight, which could be seen using the Western blot method. Pure oligosaccharide was hydrolyzed to produce various monosaccharide through incubation with HCl 4 N in 100 oC temperature for 6 hours and the result was applied on high intensity liquid chromatography incubator to learn the composition of its monosaccharide.

  19. Gas purification process

    Energy Technology Data Exchange (ETDEWEB)

    Hoelter, H.; Gresch, H.; Igelbuescher, H.; Dewert, H.

    1987-08-13

    To avoid the problems of reheating in a wet process as well as the problems of higher gas supply in a dry process, the invention proposes to separate the raw gas in two component currents, one of which undergoes wet purification while the other is led through a dry purification process. The two component currents are mixed before entering the stack. The dry chemisorption masses added in substoichiometric doses are treated in a milk-of-lime processing stage, after which the reacted and non-reacted chemisorption masses are treated by wet purification and then by oxidation.

  20. Estudio de la eficiencia operativa de las bombas eléctricas sumergibles (bes) en el campo v.h.r. en base a las curvas de operación

    OpenAIRE

    2009-01-01

    Dada la gran necesidad de incrementar la producción en el distrito Amazónico, se propuso realizar un estudio de la eficiencia operativa de las bombas electrosumergible en el campo, en el cual se recopila toda la información relacionada con las bombas del campo, análisis de la información y determinación de posibles soluciones a las deficiencias operativas que permitan optimizar el proceso y obtener mayor producción de petróleo en el campo, que será de gran beneficio para la empresa y el país...

  1. Evaluación de dos bombas hidráulicas de engranajes de desplazamientos 32 y 50 cm3/rev

    OpenAIRE

    Pedro Paneque Rondón; Eugenio Romantchik Kriuskova; Luciano Pérez Sobrevilla; Remigio Durán Hernández; Ricardo Castillo Calderín

    2013-01-01

    Como parte de las investigaciones que se llevan a cabo en la Universidad Agraria de La Habana (UNAH), en el Centro de Mecanización Agro- pecuaria (CEMA), con la cooperación de la Universidad Autónoma Chapingo (UACh), en el Departamento de Ingeniería Mecánica Agrícola (DIMA), dirigidas al desarrollo de la Oleohidráulica, se sometieron a evaluación dos bombas de engranajes de la marca Caproni de fabricación búlgara, con desplazamientos o cilindradas de 32 y 50 cm3/rev, refiriéndose la prueba a ...

  2. Modelado, simulación y cálculo estructural de una bomba de pistones : aplicación docente

    OpenAIRE

    Cuesta Viñolo, Rodrigo

    2010-01-01

    En este proyecto se estudia un bomba hidráulica de pistones, realizando su modelado mediante un programa de diseño, el Solid Edge ST, y analizando lo modos de vibración de sus elementos con un software de elementos finitos, Femap. Se ha elaborado una aplicación docente destinada a escuelas de ingeniería, con el fin de mostrar los componentes, su función, la metodología de montaje, su funcionamiento y los posibles puntos críticos en su diseño. El objetivo principal de este proyecto fin de carr...

  3. Avaliação do volume de fluxo venoso da bomba sural por ultra-sonografia Doppler durante cinesioterapia ativa e passiva: um estudo piloto

    OpenAIRE

    Campos,Carmindo Carlos Cardoso; Albuquerque,Patrícia Cavalcanti de; Braga,Ivson José da Silva

    2008-01-01

    CONTEXTO: O fisioterapeuta na unidade hospitalar atua sobre os efeitos da hipoatividade ou inatividade do paciente acamado. Na prática diária, a contração do músculo da panturrilha é difundida entre os profissionais de saúde no ambiente hospitalar, principalmente nos períodos de pré e pós-operatório, como forma de diminuir a estase venosa e os riscos de trombose venosa profunda nos membros inferiores. OBJETIVO: Avaliar o volume de fluxo venoso na bomba sural, através de ultra-sonografia doppl...

  4. Detección de cavitación en una bomba centrífuga usando emisiones acústicas

    OpenAIRE

    Quiroga M.,Jabid; Oviedo C,Silvia; García C,Alfonso

    2012-01-01

    En el presente artículo se propone el uso de las emisiones acústicas para el monitoreo de la cavitación en una bomba centrífuga. Este monitoreo se ejecuta a través del seguimiento a unos indicadores de falla obtenidos a partir del valor RMS de la señal de emisiones acústicas en dominio tiempo y el valor RMS de los coeficientes de la Transformada Discreta Wavelet (TDW) usando la onda madre db6 de la misma señal acústica. La experimentación se realiza en un banco dedicado que permite cavitar a ...

  5. Estudio mediante CFD de la interacción rotor-estator en una bomba centrífuga con difusor

    OpenAIRE

    Coussirat Núñez, Miguel Gustavo; Fontanals García, Alfred; Guardo Zabaleta, Alfredo de Jesús

    2012-01-01

    Una bomba centrífuga con difusor, trabajando en diseño óptimo es estudiada por modelado numérico (CFD) con el fin de identificar y estudiar la posible amplificación de fluctuaciones de presión dentro de la máquina (RSI). Se usan resultados numéricos previos, obtenidos en álabes aislados y cascadas de álabes fijos y móviles con el objeto de validar/calibrar los modelos para la turbulencia a utilizar, comparando con los de bases de datos experimentales. Este trabajo previo luego ...

  6. Determinación de los coeficientes de carga y capacidad para bombas centrífugas que manipulan fluidos con propiedades diferentes a las del agua

    Directory of Open Access Journals (Sweden)

    Juan Rafael Mestizo Ceron

    2004-01-01

    Full Text Available A partir de un análisis de la influencia de las propiedades del fluido, tales como la viscosidad y la densidad, se obtienen los coeficientes de carga y capacidad en las características de las bombas centrífugas, según las propiedades del fluido que se manipula, las características constructivas y los parámetros requeridos del equipo de bombeo. El empleo de los coeficientes obtenidos mejora considerablemente las condiciones para la simplificación de los cálculos y análisis correspondientes y logra una mayor precisión.

  7. Evaluación de riesgos sobre la reparación de una bomba de refinería petrolífera

    OpenAIRE

    Rión-Vallés, Jordi

    2014-01-01

    El trabajo parte de la hipótesis de una reparación real de una bomba centrífuga en una refinería de petróleo. A partir de este hecho se realiza una evaluación general de riesgos para la seguridad y salud de aquellos trabajadores que se ven involucrados directamente con las diferentes tareas que abarca el proceso de reparación. Nos encontramos ante una empresa con gran interés para garantizar la seguridad de los trabajadores, pero aun así, todavía queda mucho por hacer. Por este motivo, la ...

  8. Overview of the purification of recombinant proteins.

    Science.gov (United States)

    Wingfield, Paul T

    2015-04-01

    When the first version of this unit was written in 1995, protein purification of recombinant proteins was based on a variety of standard chromatographic methods and approaches, many of which were described and mentioned throughout Current Protocols in Protein Science. In the interim, there has been a shift toward an almost universal usage of the affinity or fusion tag. This may not be the case for biotechnology manufacture where affinity tags can complicate producing proteins under regulatory conditions. Regardless of the protein expression system, questions are asked as to which and how many affinity tags to use, where to attach them in the protein, and whether to engineer a self-cleavage system or simply leave them on. We will briefly address some of these issues. Also, although this overview focuses on E.coli, protein expression and purification, other commonly used expression systems are mentioned and, apart from cell-breakage methods, protein purification methods and strategies are essentially the same.

  9. One-step purification of E. coli elongation factor Tu

    DEFF Research Database (Denmark)

    Knudsen, Charlotte Rohde; Clark, Brian F. C.; Degn, B

    1993-01-01

    The tuf A gene, encoding the E. coli elongation factor Tu, was cloned in the pGEX gene fusion system. Upon expression EF-Tu is fused to glutathione-S-transferase serving as a purification handle with affinity for glutathione immobilised on agarose. This allows purification of EF-Tu in a one...

  10. One-step purification of E. coli elongation factor Tu

    DEFF Research Database (Denmark)

    Knudsen, Charlotte Rohde; Clark, Brian F. C.; Degn, B

    1993-01-01

    The tuf A gene, encoding the E. coli elongation factor Tu, was cloned in the pGEX gene fusion system. Upon expression EF-Tu is fused to glutathione-S-transferase serving as a purification handle with affinity for glutathione immobilised on agarose. This allows purification of EF-Tu in a one...

  11. Cost effective purification of intein based syntetic cationic antimicrobial peptide expressed in cold shock expression system using salt inducible E. coli GJ1158

    Directory of Open Access Journals (Sweden)

    Seetha Ram Kotra

    2014-03-01

    Full Text Available Objective:Synthetic cationic antimicrobial peptide (SC-AMP is an important and upcoming therapeutic molecule against onventional antibiotics. In this study, an attempt was made to purify the SC-AMP without the enzymatic cleavage of the affinity tag, by using an intein-based system. Methods:The intein sequence was amplified from pTYB11 vector using PCR methodologies and the N-terminal of intein was ligated with SC-AMP. The designed construct, intein-SC-AMP was cloned into MCS region of cold shock expression vector, pCOLDI and the recombinant peptide was purified on a chitin affinity column by cleaving intein with 50 mM DTT without applying enzymatic cleavage. Later the peptide was quantified and its antibacterial activity of the purified peptide was studied using well diffusion method. Results: Initially, intein-SC-AMP was expressed as a fusion protein in both IPTG inducible E. coli BL21(DE3 and salt inducible E. coli GJ1158. Single step purification using CBD (chitin binding domain - intein tag in salt inducible E. coli GJ1158, yields the SC-AMP in the soluble form at a oncentration of 208 mg/L. The antibacterial activity and minimal inhibitory concentration (MIC of the purified SC-AMP was studied against both Gram positive and Gram negative microorganisms. Conclusion: For the first time, single step purification of soluble SC-AMP was carried out using chitin-binding domain affinity tag in salt inducible E. coli GJ1158 without an application of enzymatic cleavage. J Microbiol Infect Dis 2014;4(1:13-19

  12. Photocatalytic materials and technologies for air purification.

    Science.gov (United States)

    Ren, Hangjuan; Koshy, Pramod; Chen, Wen-Fan; Qi, Shaohua; Sorrell, Charles Christopher

    2017-03-05

    Since there is increasing concern for the impact of air quality on human health, the present work surveys the materials and technologies for air purification using photocatalytic materials. The coverage includes (1) current photocatalytic materials for the decomposition of chemical contaminants and disinfection of pathogens present in air and (2) photocatalytic air purification systems that are used currently and under development. The present work focuses on five main themes. First, the mechanisms of photodegradation and photodisinfection are explained. Second, system designs for photocatalytic air purification are surveyed. Third, the photocatalytic materials used for air purification and their characteristics are considered, including both conventional and more recently developed photocatalysts. Fourth, the methods used to fabricate these materials are discussed. Fifth, the most significant coverage is devoted to materials design strategies aimed at improving the performance of photocatalysts for air purification. The review concludes with a brief consideration of promising future directions for materials research in photocatalysis. Copyright © 2016 Elsevier B.V. All rights reserved.

  13. Desarrollo de un prototipo de bomba de flujo pulsátil para caracterizar las condiciones hidrodinámicas en un ambiente de circulación extracorpórea Development of a prototype of pulsatile flow pump for characterizing the hydrodinamic conditions in an extracorporeal circulation setting

    Directory of Open Access Journals (Sweden)

    John Bustamante O

    2007-02-01

    Full Text Available Es difícil generar con bombas convencionales las condiciones de flujo del sistema cardiovascular, con las cuales se emulen condiciones hemodinámicas para evaluar diferentes dispositivos interpuestos a la corriente sanguínea. Para resolver este inconveniente, se desarrolló un sistema automatizado de bombeo de flujo pulsátil, conformado por un controlador difuso que captura datos de los instrumentos de monitoreo de variables hidrodinámicas a través de una interfaz de adquisición, y aplica señales de control a una bomba de flujo pulsátil. Este sistema permite hacer pruebas hidromecánicas que brindan elementos para interpretar la influencia de las variables que intervienen en el flujo pulsante y simular condiciones hemodinámicas en un ambiente de circulación extracorpórea. Mediante técnicas de diseño asistido por computador, se construyó una bomba de diafragma accionada neumáticamente. El controlador se desarrolló mediante la técnica de Fuzzi Control®, el cual regula el pulso y el flujo de acuerdo con parámetros y datos capturados con la interfaz de adquisición. Las pruebas del prototipo se realizaron en un laboratorio de fluidos variando frecuencia, resistencia hidráulica, viscosidad y presión de pulso, imitando las condiciones hemodinámicas de un adulto y usando como fluido de trabajo una solución acuosa con 5 cPo a 370C, para simular la viscosidad sanguínea a temperatura corporal. La reproducción de una bomba que sirve para analizar el efecto de las variables en el flujo pulsante, puede usarse en el estudio de diferentes dispositivos intravasculares y ayudar a refinar aspectos técnicos y funcionales en el estudio preliminar de máquinas de circulación extracorpórea.With conventional pumps, it is difficult to generate the cardiovascular system conditions that may emulate hemodynamic conditions for evaluating different devices interposed to the blood flow. In order to resolve this inconvenient, an automated pulsatile

  14. High-Yield Soluble Expression and Simple Purification of the Antimicrobial Peptide OG2 Using the Intein System in Escherichia coli

    Directory of Open Access Journals (Sweden)

    Yong-Gang Xie

    2013-01-01

    Full Text Available OG2 is a modified antimicrobial peptide, that is, derived from the frog peptide Palustrin-OG1. It has high antimicrobial activity and low cytotoxicity, and it is therefore promising as a therapeutic agent. Both prokaryotic (Escherichia coli and eukaryotic (Pichia pastoris production host systems were used to produce OG2 in our previous study; however, it was difficult to achieve high expression yields and efficient purification. In this study, we achieved high-yield OG2 expression using the intein fusion system. The optimized OG2 gene was cloned into the pTWIN1 vector to generate pTWIN-OG2-intein2 (C-terminal fusion vector and pTWIN-intein1-OG2 (N-terminal fusion vector. Nearly 70% of the expressed OG2-intein2 was soluble after the IPTG concentration and induction temperature were decreased, whereas only 42% of the expressed of intein1-OG2 was soluble. Up to 75 mg of OG2-intein2 was obtained from a 1 l culture, and 85% of the protein was cleaved by 100 mM DTT. Intein1-OG2 was less amenable to cleavage due to the inhibition of cleavage by the N-terminal amino acid of OG2. The purified OG2 exhibited strong antimicrobial activity against E. coli K88. The intein system is the best currently available system for the cost-effective production of OG2.

  15. PUMP-PUMP FOUR-WAVE MIXING IN DISTRIBUTED FIBER RAMAN AMPLIFIERS MEZCLA DE CUATRO ONDAS ENTRE BOMBAS EN AMPLIFICADORES DE FIBRA RAMAN DISTRIBUIDOS

    Directory of Open Access Journals (Sweden)

    Marcelo Soto

    2007-08-01

    Full Text Available In this work, a comprehensive mathematical model which rigorously describes the interaction between stimulated Raman scattering (SRS and four-wave mixing (FWM in distributed fiber Raman amplifiers (DFRAs is presented. The parametric effects on DFRAs due to pump-pump FWM processes are completely characterized. Numerical simulations are contrasted with experimental measurements which were carried out with different configurations of DFRAs. The results validate the proposed mathematical model, which can be extended to include other non-linear effects. Finally, the pump-to-pump FWM effects on the spectral gain of a DFRA are analyzed.En este trabajo se presenta un modelo matemático que describe rigurosamente la interacción entre esparcimiento Raman estimulado (SRS y mezcla de cuatro ondas (FWM en amplificadores Raman distribuidos (DFRAs. Los efectos paramétricos ocurridos en DFRAs debido al proceso de FWM entre bombas, son completamente caracterizados. Los resultados de las simulaciones numéricas son contrastados con mediciones experimentales llevadas a cabo en diferentes configuraciones de DFRAs. Los resultados permiten validar el modelo matemático propuesto, el cual puede ser extendido para incluir otras no-linealidades. Finalmente, se analizan los efectos de FWM entre bombas en el comportamiento espectral de la ganancia de un DFRA.

  16. Propuesta de dispositivo para diagnosticar las bombas de alta presión de los grupos electrógenos MTU serie 4000

    Directory of Open Access Journals (Sweden)

    Elio Rafael Hidalgo-Batista

    2015-01-01

    Full Text Available Se presenta el diseño de un dispositivo para el diagnóstico de las bombas de alta presión de combustible de motores de co mbustión interna Diesel de los Grupos Electrógenos MTU Br 4 000 16V G81 , los cuales pertenecen a la Unidad Empresarial de Base de Generación Distribuida de la Empresa Eléctrica Holguín, siendo este el primer objetivo del proyecto, el segundo es la elaborac ión de los pasos necesarios para el montaje y la realización del diagnóstico de las bombas en el banco. El dispositivo obtenido del diseño se montará en la bancada de un torno universal y para el mismo se utilizaran planchas de acero al carbono. El mismo p ermitirá diagnosticar fallas tales como: baja presión en el conducto común, salideros de combustible y goteo de combustible. El valor de la presión existente en el conducto común ( Common Rail se conocerá a través del valor del voltaje medido en el sensor.

  17. SFC在分子筛纯化系统切换控制中的应用%Application of SFC in switching-over control of molecular sieve purification system

    Institute of Scientific and Technical Information of China (English)

    付定君; 伍晓娟; 徐平林

    2011-01-01

    针对分子筛纯化系统切换控制的特点,结合横河CETUMCS3000控制系统中的顺序控制功能,介绍了SFC在分子筛纯化系统切换控制中的应用,以及一些组态经验和维护措施。%Application of SFC in switching-over control of molecular sieve purification system is briefed on basis of the features of switching-over control of molecular sieve purification system in combination with function of sequence control of Henghe CETUM CS3000 control system and experiences on configuration and maintenance measures are described.

  18. 161. Implantación y destete de una bomba axial implantable (incor por una miocardiopatía tóxica: primer caso en españa

    Directory of Open Access Journals (Sweden)

    E. Castells Cuch

    2010-01-01

    Comentarios: La técnica de implantación y explantación presentadas no es muy compleja y es reproducible. La aplicación de bombas axiales está obteniendo muy buenos resultados, por lo que creemos que su uso debe generalizarse.

  19. Study on the thermodynamics of the gadolinium-hydrogen binary system (H/Gd = 0.0–2.0) and implications to metallic gadolinium purification

    Energy Technology Data Exchange (ETDEWEB)

    Fu, Kai; Li, Guoling [Beijing National Laboratory of Molecular Sciences, State Key Laboratory of Rare Earth Materials Chemistry and Applications, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871 (China); Department of Materials Physics and Chemistry, University of Science and Technology Beijing, NO. 30, Xueyuan Road, Beijing 100083 (China); Li, Jigang [Beijing National Laboratory of Molecular Sciences, State Key Laboratory of Rare Earth Materials Chemistry and Applications, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871 (China); Liu, Yang [Department of Food Science, College of Arts and Science, Beijing Union University, Beijing, 100101 (China); Tian, Wenhuai, E-mail: wenhuaitian@ustb.edu.cn [Department of Materials Physics and Chemistry, University of Science and Technology Beijing, NO. 30, Xueyuan Road, Beijing 100083 (China); Zheng, Jie [Beijing National Laboratory of Molecular Sciences, State Key Laboratory of Rare Earth Materials Chemistry and Applications, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871 (China); Li, Xingguo, E-mail: xgli@pku.edu.cn [Beijing National Laboratory of Molecular Sciences, State Key Laboratory of Rare Earth Materials Chemistry and Applications, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871 (China)

    2016-07-15

    The thermodynamics of the gadolinium-hydrogen (Gd–H) binary system (H/Gd = 0.0–2.0) between 650 and 900 °C is studied by pressure composition isotherm measurement. Significant H dissolution in Gd is observed, up to H/Gd = 0.34 at 650 °C and 0.55 at 900 °C. The metal-rich phase boundary of nonstoichiometric gadolinium dihydride was found to occur with nominal composition of GdH{sub 1.80} at 650 °C and GdH{sub 1.53} at 900 °C. The results are in fairly good agreement with previous experimental work but with improved accuracy. The binary phase diagram is obtained using the CALPHAD method. The thermodynamic study here assists the understanding on the efficient deoxygenation effect by the H in Gd for Gd purification. - Highlights: • PCI measurements with high accuracy has been carried out. • A first assessment of Gd–H system has been carried out by the CALPHAD method. • A set of self-consistent thermodynamic parameters was derived for this system.

  20. Application of Moving Bed Biofilm Reactor (MBBR) and Integrated Fixed Activated Sludge (IFAS) for Biological River Water Purification System: A Short Review

    Science.gov (United States)

    Lariyah, M. S.; Mohiyaden, H. A.; Hayder, G.; Hayder, G.; Hussein, A.; Basri, H.; Sabri, A. F.; Noh, MN

    2016-03-01

    This review paper present the MBBR and IFAS technology for urban river water purification including both conventional methods and new emerging technologies. The aim of this paper is to present the MBBR and IFAS technology as an alternative and successful method for treating different kinds of effluents under different condition. There are still current treatment technologies being researched and the outcomes maybe available in a while. The review also includes many relevant researches carried out at the laboratory and pilot scales. This review covers the important processes on MBBR and IFAS basic treatment process, affecting of carrier type and influent types. However, the research concluded so far are compiled herein and reported for the first time to acquire a better perspective and insight on the subject with a view of meeting the news approach. The research concluded so far are compiled herein and reported for the first time to acquire a better perspective and insight on the subject with a view of meeting the news approach. To this end, the most feasible technology could be the combination of advanced biological process (bioreactor systems) including MBBR and IFAS system.

  1. Separation and purification of isorhamnetin 3-sulphate from Flaveria bidentis (L.) Kuntze by counter-current chromatography comparing two kinds of solvent systems.

    Science.gov (United States)

    Xie, Qianqian; Yin, Li; Zhang, Guoliang; Wei, Yun

    2012-01-01

    The first preparative separation of a flavonoid sulphate isorhamnetin 3-sulphate from Flaveria bidentis (L.) Kuntze by counter-current chromatography (CCC) was presented. Two kinds of solvent systems were used. A conventional organic/aqueous solvent system n-butanol-ethyl acetate-water (4:1:5, v/v) was used, yielding isorhamnetin 3-sulphate 2.0 mg with a purity of 93.4% from 83 mg of pre-enriched crude extract obtained from 553 mg ethanol extract by macroporous resin. A one-component organic/salt-containing system composed of n-butanol-0.25% sodium chloride aqueous solution (1:1, v/v) was also used, and the LC column packed with macroporous resin has been employed for desalination of the target compound purified from CCC. As a result, 2.1 mg of isorhamnetin 3-sulphate with a purity of over 97% has been isolated from 402 mg of crude extract without pre-enrichment. Compared with the conventional organic/aqueous system, the one-component organic/salt-containing aqueous system was more suitable for the separation of isorhamnetin 3-sulphate, and purer target compound was obtained from the crude extract without pre-enrichment using the new solvent system. The chemical structure was confirmed by ESI-MS and (1)H, (13)C NMR. In summary, our results indicated that CCC using one-component organic/salt-containing aqueous solution is very promising and powerful for high-throughput purification of isorhamnetin 3-sulphate from Flaveria bidentis (L.) Kuntze.

  2. Enterovirus 71 Virus Propagation and Purification

    OpenAIRE

    Kristin L Shingler; Organtini, Lindsey J.; Hafenstein, Susan

    2014-01-01

    Since its discovery in 1969, enterovirus 71 (EV71) has emerged as a serious worldwide health threat. This member of the picornavirus family causes hand, foot, and mouth disease, and also has the capacity to invade the central nervous system to cause severe disease and death. This is the propagation and purification procedure to produce infectious virion.

  3. Heat pumps in dairy property; Bombas de calor em propriedade leiteira

    Energy Technology Data Exchange (ETDEWEB)

    Baldassin Junior, Ricardo [Universidade Estadual de Campinas (FEM/UNICAMP), SP (Brazil). Fac. de Engenharia Mecanica. Planejamento de Sistemas Energeticos; Cortez, Luis Augusto Barbosa; Jordan, Rodrigo Aparecido; Ferreira, Tamara Aurora Bartholomei [Universidade Estadual de Campinas (FEAGRI/UNICAMP), SP (Brazil). Fac. de Engenharia Agricola. Dept. de Construcoes Rurais e Ambiencia; Neves Filho, Lincoln de Carmargo; Silveira Junior, Vivaldo [Universidade Estadual de Campinas (FEA/UNICAMP), SP (Brazil). Fac. de Engenharia de Alimentos

    2006-07-01

    Milk is a prominence product of Brazilian agriculture, with about 2/3 of the rural properties dedicated to the milkmaid cattle breeding, the second bigger flock of the world and a representative participation in the national economy. In spite of its size, the sector still suffers of low productivity, high costs, the instability of prices and technological delay, which resulted in many producers have abandoned its activities in consequence of this new competitive scenario. Nowadays, subjects as, the rational use of energy and intensive application of technology have been hardly discussed, and indicated, as important aspects to obtain high productivity gains. In this work, it is presented an evaluation of the use of energy in a milkmaid which processes cooled raw milk in Morungaba city, Sao Paulo state, Brazil, with a 1.000 liters daily production, making use of the three main processes (cooling, milking system and water heating). Aiming at to promote the rational use of energy, it was realized in this property, an adequacy in the tank milk cooler to utilize the condensation heat, of the refrigeration system, to heat cleanness water (operation according to a heat pump). Through this practical, it was objectified the exclusion of the use of the electric resistance in the heating process for term -accumulation (300 liters/day), promoting consumption reduction and rational use of energy. (author)

  4. Crystallization using reverse micelles and water-in-oil microemulsion systems: the highly selective tool for the purification of organic compounds from complex mixtures.

    Science.gov (United States)

    Kljajic, Alen; Bester-Rogac, Marija; Klobcar, Andrej; Zupet, Rok; Pejovnik, Stane

    2013-02-01

    The active pharmaceutical ingredient orlistat is usually manufactured using a semi-synthetic procedure, producing crude product and complex mixtures of highly related impurities with minimal side-chain structure variability. It is therefore crucial for the overall success of industrial/pharmaceutical application to develop an effective purification process. In this communication, we present the newly developed water-in-oil reversed micelles and microemulsion system-based crystallization process. Physiochemical properties of the presented crystallization media were varied through surfactants and water composition, and the impact on efficiency was measured through final variation of these two parameters. Using precisely defined properties of the dispersed water phase in crystallization media, a highly efficient separation process in terms of selectivity and yield was developed. Small-angle X-ray scattering, high-performance liquid chromatography, mass spectrometry, and scanning electron microscopy were used to monitor and analyze the separation processes and orlistat products obtained. Typical process characteristics, especially selectivity and yield in regard to reference examples, were compared and discussed. Copyright © 2012 Wiley Periodicals, Inc.

  5. Escherichia coli-based expression system for the heterologous expression and purification of the elicitin β-cinnamomin from Phytophthora cinnamomi.

    Science.gov (United States)

    Hofzumahaus, Sebastian; Schallmey, Anett

    2013-08-01

    Elicitins are sterol carrier proteins from the Oomycete genera Phytophthora and Phytium and elicit a hypersensitive response in many economically important plants, in some cases causing a systemic acquired resistance. Their recombinant expression in bacteria is complicated by the presence of three disulfide bonds in the elicitin structure. In consequence, elicitins have so far only been produced in soluble form by isolation from native Phytophthora or Phytium strains or by recombinant expression in the yeast Pichia pastoris. Here, for the first time, we report the soluble expression of the elicitin β-cinnamomin from Phytophthora cinnamomi in Escherichia coli by secretion of the protein into the periplasm. β-Cinnamomin yields have been significantly improved after careful selection of the optimum secretion signal sequence. In total, 17.6 mg β-cinnamomin per liter cell culture have been obtained in shake flasks with the secretion signal sequence of the maltose-binding protein MalE from E. coli. Furthermore, by making use of a C-terminal His-tag, β-cinnamomin purification has been significantly simplified with only one step of immobilized metal ion affinity chromatography yielding protein of high purity (>90%). The established protocol has further been successfully applied to the soluble expression of another elicitin.

  6. 用FPLC系统提纯可溶型Ⅱ型胶原蛋白%Isolation and purification of soluble typeⅡcollagen with FPLC system

    Institute of Scientific and Technical Information of China (English)

    钱娴娟; 胡永秀; 赵文明

    2001-01-01

    Objective To isolate and purify soluble type Ⅱcollagen by FPLCsystem. Methods Xiphoid cartilage of chickens was digested with pepsin and was centrifuged to obtain the rough protein solution. Then, FPLC system was adopted to carry on DEAE ion exchange chromatography and desalting chromatography in order to purify the protein. Results It was found that the bands of CⅡsamples and standard CⅡwere at the same location by SDS-PAGE. Conclusions The improved method we adopted had significant advantages such as the high purification efficiency,excellent stability and the fast and simple working process compared with traditional method.%目的 采用FPLC系统提取纯化可溶型Ⅱ型胶原蛋白(CⅡ)。方法 采用鸡剑突软骨,经胃蛋白酶消化离心,再使用FPLC系统进行DEAESepharoseFF高效液相离子交换层析和脱盐柱脱盐,得到纯化的CⅡ。结果 自提的CⅡ纯品与CⅡ标准品电泳结果带型一致。结论 本法提纯蛋白效率高,重复性好,快速简便。

  7. Purification of contaminated groundwater by membrane technology

    Energy Technology Data Exchange (ETDEWEB)

    Youn, In Soo; Chung, Chin Ki; Kim, Byoung Gon [Korea Institute of Geology Mining and Materials, Taejon (Korea, Republic of)

    1996-12-01

    The objective of this study is to apply the membrane separation technology to the purification of contaminated ground water in Korea. Under this scope, the purification was aimed to the drinking water level. The scale of the membrane system was chosen to a small filtration plant for local clean water supplies and/or heavy purifiers for buildings and public uses. The actual conditions of ground water contamination in Korea was surveyed to determine the major components to remove under the drinking water requirements. To set up a hybrid process with membrane methods, conventional purification methods were also investigated for the comparison purpose. The research results are summarized as follows : 1) Contamination of the groundwater in Korea has been found to be widespread across the country. The major contaminant were nitrate, bacteria, and organic chlorides. Some solvents and heavy metals are also supposed to exist in the ground water of industrial complexes, cities, and abandoned mines. 2) The purification methods currently used in public filtration plants appear not to be enough for new contaminants from recent industrial expanding. The advanced purification technologies generally adopted for this problem have been found to be unsuitable due to their very complicated design and operation, and lack of confidence in the purification performance. 3) The reverse osmosis tested with FilmTec FT30 membrane was found to remove nitrate ions in water with over 90 % efficiency. 4) The suitable membrane process for the contaminated groundwater in Korea has been found to be the treatments composed of activated carbon, microfiltration, reverse osmosis or ultrafiltration, and disinfection. The activated carbon treatment could be omitted for the water of low organic contaminants. The microfiltration and the reverse osmosis treatments stand for the conventional methods of filtration plants and the advanced methods for hardly removable components, respectively. It is recommended

  8. Destilación al Vacío de Etanol usando Bomba Chorro

    Directory of Open Access Journals (Sweden)

    William Orozco

    2010-12-01

    Full Text Available This paper presents a review and analysis of the literature regarding ethanol production technologies and, the features, dimensions and functioning of jet pumps, which are devices that create a vacuum for distilling ethanol. This review mainly takes into account references from the previous 15 years, both national and international research, which yielded updated information on the use of jet pumps in refrigeration and air conditioning systems and established that, in terms of vacuum distillation of ethanol the best operating conditions and, therefore, the best dimensions and geometry of the jet pump, are obtained for the driving pressure and pressure at the exit nozzle to deliver a 6 kPa vacuum with the greatest entrainment ratio and the highest Mach number at the exit nozzle.

  9. A Novel Aqueous Two Phase System Composed of a Thermo-Separating Polymer and an Organic Solvent for Purification of Thermo-Acidic Amylase Enzyme from Red Pitaya (Hylocereus polyrhizus Peel

    Directory of Open Access Journals (Sweden)

    Mehrnoush Amid

    2014-05-01

    Full Text Available The purification of thermo-acidic amylase enzyme from red pitaya (Hylocereus polyrhizus peel for the first time was investigated using a novel aqueous two-phase system (ATPS consisting of a thermo-separating copolymer and an organic solvent. The effectiveness of different parameters such as molecular weight of the thermo-separating ethylene oxide-propylene oxide (EOPO copolymer and type and concentration of organic solvent on the partitioning behavior of amylase was investigated. In addition, the effects of phase components, volume ratio (VR, pH and crude load of purification factor and yield of amylase were evaluated to achieve the optimum partition conditions of the enzyme. In the novel ATPS method, the enzyme was satisfactorily partitioned into the polymer-rich top phase in the system composed of 30% (w/w EOPO 2500 and 15% (w/w 2-propanol, at a volume ratio of 1.94 and with a crude load scale of 25% (w/w at pH 5.0. Recovery and recycling of components was also measured in each successive step of the ATPS process. The enzyme was successfully recovered by the method with a high purification factor of 14.3 and yield of 96.6% and copolymer was also recovered and recycled at a rate above 97%, making the method was more economical than the traditional ATPS method.

  10. A novel aqueous two phase system composed of a thermo-separating polymer and an organic solvent for purification of thermo-acidic amylase enzyme from red pitaya (Hylocereus polyrhizus) peel.

    Science.gov (United States)

    Amid, Mehrnoush; Manap, Yazid; Zohdi, Nor Khanani

    2014-05-22

    The purification of thermo-acidic amylase enzyme from red pitaya (Hylocereus polyrhizus) peel for the first time was investigated using a novel aqueous two-phase system (ATPS) consisting of a thermo-separating copolymer and an organic solvent. The effectiveness of different parameters such as molecular weight of the thermo-separating ethylene oxide-propylene oxide (EOPO) copolymer and type and concentration of organic solvent on the partitioning behavior of amylase was investigated. In addition, the effects of phase components, volume ratio (VR), pH and crude load of purification factor and yield of amylase were evaluated to achieve the optimum partition conditions of the enzyme. In the novel ATPS method, the enzyme was satisfactorily partitioned into the polymer-rich top phase in the system composed of 30% (w/w) EOPO 2500 and 15% (w/w) 2-propanol, at a volume ratio of 1.94 and with a crude load scale of 25% (w/w) at pH 5.0. Recovery and recycling of components was also measured in each successive step of the ATPS process. The enzyme was successfully recovered by the method with a high purification factor of 14.3 and yield of 96.6% and copolymer was also recovered and recycled at a rate above 97%, making the method was more economical than the traditional ATPS method.

  11. Gasolimp: biodegradable protector for gasoline pumps; Gasolimp: protetor biodegradavel para bomba de gasolina

    Energy Technology Data Exchange (ETDEWEB)

    Vinas Cortez, Juan Carlos [Universidade Federal da Paraiba (UFPB), Joao Pessoa, PB (Brazil). Dept. de Psicologia. Setor Organizacional do Trabalho

    2004-07-01

    It is made from an absorbent material that has natural fibers and cellulose in its composition. The polyurethane bio-foam presented excellent levels of absorption of the toxic residues left by gasoline such as carbon monoxide, hydrocarbons, oxygen, nitrogen and sulfur. All the materials used in the composition of Gasolimp are biodegradable. After a four-year research period we found out that from five to eight drops of gasoline are spilt at the moment that car pump is being filled up and they end up either in the cloth the attendant holds in his hand, on his hand, on his clothes, on the car paint or on the soil. The research shows that the toxic effects the gasoline hydrocarbons cause health problems to the attendants such as headaches, lesions on their hands and eyes, dizziness, gastro-intestine problems, heart palpitation, breathing problems and can even affect the central nerve system. The final use of the product has the utmost importance: after thirty days of use Gasolimp must be replaced and when re-used it may be sent to mills and earthenware factories and there it will function as a product that will co-generate power. (author)

  12. Using aerated gravel-packed contact bed and constructed wetland system for polluted river water purification: A case study in Taiwan

    Science.gov (United States)

    Lin, J. L.; Tu, Y. T.; Chiang, P. C.; Chen, S. H.; Kao, C. M.

    2015-06-01

    The Ju-Liao Stream is one of the most contaminated streams in Kaohsiung City, Taiwan. A constructed wetland (CW) system was built in 2010 for polluted stream water purification and ecosystem improvement. An aerated gravel-packed contact bed (CB) system was built in 2011 and part of the stream water was treated by the CB before discharging to the CW. The influent rates of the CW and CB were approximately 5570 and 900 m3/d, respectively. The CW contained one free-water surface basin planted with emergent wetland plants, followed by the plug-flow channel-shaped free-water surface basin planted with emergent and floating wetland plants. The mean measured hydraulic loading rate (HLR), hydraulic retention time (HRT), water depth, and total volume of wetland system were 1.7 m/d, 0.68 d, 0.7 m, and 4400 m3, respectively. The aeration zone of the CB system had a dimension of 24 m (L) × 8 m (W) × 3 m (H), which was filled with gravels (average diameter = 5 cm) with a porosity of 0.4, and the aeration rate was 7.8 m3/min. Results show that the CB system was able to remove 69% of suspended solid (SS), 86% of biochemical oxygen demand (BOD), and 58% of total nitrogen (TN). Up to 82% of BOD and 27% of TN could be removed in the CW system. Removal efficiency of SS was affected by the growth of chlorophyll a in the CW system due to the growth of algae. The observed first-order decay rates (k) for BOD and TN in CB were 9.3 and 4.2 1/d, and the k values for BOD and TN removal in CW were 2.5 and 0.45 1/d. The high pollutant removal efficiencies in the CB system indicate that the system could enhance the organic and nutrient removal through the biological processes effectively. Sediments contained high total organic matter (1.9-4.5%), sediment total nitrogen (6.4-10.1 g/kg), sediment total phosphorus (0.59-0.94 g/kg), and sediment oxygen demand (0.9-4.1 g O2/m2 d). The organic and nutrient-abundant sediments resulted in reduced conditions (oxidation-reduction potential measurements

  13. Pond systems for the purification and disposal of domestic wastewater from small communities: use, design, operation and maintenance

    CSIR Research Space (South Africa)

    Drews, RJLC

    1983-01-01

    Full Text Available Pond systems are ideally suited for small communities, and for schools, hospitals and other institutions since they are simple and economical to construct, operate and maintain. Numerous pons systems have been designed and commissioned in South...

  14. Computational program to design heat pumps by compression (ciclo 1.0); Programa computacional para diseno de bombas de calor por compresion (ciclo 1.0)

    Energy Technology Data Exchange (ETDEWEB)

    De Alba Rosano, Mauricio [CIE, UNAM, Temixco, Morelos (Mexico)

    2000-07-01

    A new computational program has been developed in order to design single stage compression heat pumps. This software, named CICLO 1.0 allows the design of water-water, water-air, air-water and air-air heat pumps, for industrial and residential applications. CICLO 1.0 simulates three types of compressors: reciprocating, screw and scroll. Also has a data base created with REFPROP software which includes eleven refrigerants. The condenser and evaporator simulation includes global conductance (UA) determination, and when one or both are shell and tube's type, this software shows the even number of tube passes by shell. The software determines the best compressor and refrigerant setup taking the COP as a parameter; in order to obtain this, is necessary to know the inlet/outlet conditions of the fluid to be heated, the inlet conditions of the fluid that gives heat, and the electric motor efficiency that drives the compressor. The afforded results by CICLO 1.0 are: operation conditions from compression cycle, that means, pressures and temperatures at the inlet/outlet from every heat pump component are determined: as well as refrigerant mass flux, COP, power required by compressor, volumetric and isentropic efficiencies, heat exchangers global conductance and more data. CICLO 1.0 has been executed with heat pump data that nowadays are operating, and the results from the simulation have been very similar each other with data reported from operational facilities. [Spanish] Se ha desarrollado un nuevo programa computacional para el diseno de bombas de calor por compresion de vapor de una sola etapa. Este programa, CICLO 1.0, permite el diseno de bombas de calor de tipo: agua-agua, agua-aire, aire-agua y aire-aire, que se utilicen para aplicaciones industriales, de servicios y residenciales. CICLO 1.0 simula tres tipos de compresores: reciprocante, de tornillo y scroll: cuenta con una base de datos de refrigerantes creada con el programa REFPROP la cual incluye once

  15. Desenvolvimento de um projeto para construção de bomba-balão para contrapulsação aórtica

    OpenAIRE

    Reis,Celso Luiz dos; Évora,Paulo Roberto Barbosa; Brasil,José Carlos Franco; Ribeiro,Paulo José de Freitas; Otaviano,Adonis Garcia; Bongiovani,Hércules Lisboa; Bombonato,Rúbio; Ferez,Marcus Antônio; Sgarbieri,Ricardo Nilsson; MOREIRA NETO Francisco Fernandes; Pereira,Almir Sales; Gomes,Percival

    1989-01-01

    O presente trabalho tem por finalidade apresentar as etapas de um projeto para construção de uma bomba-balâo para contrapulsação aórtica. Desenvolveram-se três protótipos, sendo que os dois primeiros utilizavam ar comprimido e vácuo hospitalares para insuflação e deflação do cateter-balão. Esses dois protótipos apresentavam como diferenças fundamentais: o tipo de dispositivo que captava o sinal luminoso da onda R do ECG utilizado para a sincronização do bombeamento; o tipo de válvula solenóid...

  16. Desarrollo de un Sistema de Decisión basado en Lógica Borrosa para el uso de Bombas de Insulina

    Directory of Open Access Journals (Sweden)

    José B. Galván

    2014-06-01

    Full Text Available Basándonos en el conocimiento de un usuario experto en la utilización de bombas de insulina, hemos desarrollado un software de apoyo para usuarios noveles de dicha tecnología. Nuestro sistema de decisión tiene en cuenta problemas tales como la influencia de las características específicas de cada usuario, el ritmo circadiano, la actividad prevista y la glucemia preprandial. La base de nuestro sistema es un controlador borroso para el que se ha desarrollado un constructor semiautomático de reglas. El sistema ha sido probado con éxito por dos usuarios muy diferentes entre sí, lo que prueba que las ideas implementadas son válidas y suponen una interesante línea de desarrollo de sistemas de ayuda a pacientes diabéticos

  17. Identificación difusa de accionamiento de bomba centrífuga para el transporte eficiente de pulpa laterítica

    Directory of Open Access Journals (Sweden)

    Alberto Turro-Breffe

    2004-03-01

    Full Text Available En este artículo se realiza la identificación difusa de un accionamiento eléctrico de una instalación de hidrotransporte de pulpa laterítica, con el objetivo de encontrar las zonas de mejores rendimientos del sistema. Para esto se hace un estudio de los subsistemas eléctrico y mecánico a partir de la incidencia de las variables que más participan en la transferencia de energía, desde el motor de inducción hacia la bomba centrífuga. Se muestra un análisis de las funciones obtenidas, partiendo de la ecuación de los torques del accionamiento eléctrico, que permiten evaluar su eficiencia tanto en el contexto tecnológico como en el experimental.

  18. Control de eficiencia en accionamiento de bombas centrífugas para transporte de hidromezclas usando lógica difusa

    Directory of Open Access Journals (Sweden)

    Luis D Rojas Purón

    2011-02-01

    Full Text Available Se presenta un algoritmo de control difuso que tiene en cuenta las especificidades de los subsistemaseléctrico y mecánico del accionamiento. Es de interés un bloque compensador del torque electromagnéticoque mejora el trabajo del controlador difuso ante los fenómenos de cavitación que ocasiona pulsacionesdel momento y la resonancia mecánica que pueden aparecer durante el trabajo de la bomba centrífuga.Se muestran resultados experimentales de accionamientos de la planta de lixiviación de la empresaComandante Ernesto Che Guevara de Moa, con un efecto económico de aproximadamente 262 000 USDanuales por energía dejada de consumir en forma de pérdidas con el uso del controlador operando adiferentes niveles de cargas mecánicas, y que sirve para ser utilizado en accionamientos de bombas delas plantas metalúrgicas de Moa y Nicaro.  A fuzzy logic control algorithm offers the specificities of the electric subsystem and mechanic of theoperations. It is of interest a block compensator of the electromagnetic torque that improves the work ofthe fuzzy controller's before the cavitation phenomena that causes pulsations of the torque and themechanical resonance that can appear during the operation of the centrifugal pumps. Experimentalresults are shown with the controller operating at the leaching plant of the Ernesto Che Guevara Corporation,Moa for an economic effect of 262 000 USD per year. which most of the unused energy in form of losses,when the controller works to different mechanical loads and that it is good to be used in operations ofpumps of the metallurgical plants of Moa and Nicaro.

  19. Inyección de fertilizante con bombas de paletas y control por conductividad eléctrica en soluciones madre para fertirriego

    Directory of Open Access Journals (Sweden)

    Miguel Ángel Peña Peralta

    2010-01-01

    Full Text Available La inyección de fertilizantes en el fertirriego se realiza comúnmente a través de tubos Venturi y se controla con ayuda de sensores de conductividad y pH colocados en la salida del riego. Los tubos Venturi presentan problemas de caídas de presión durante la inyección. De este modo se innova con el diseño de un equipo de fertirriego controlando las perdidas de presión usando un sistema de inyección de fertilizantes por bombas de paletas rotatorias que suministran un caudal continuo. El control de la inyección lo hace un PLC Millenium II XT- 20 con un programa basado en las relaciones de conductividad eléctrica que existen entre las soluciones madre al ser mezcladas entre si y al diluirlas en agua en pruebas de laboratorio utilizando soluciones concentradas para jitomate saladet. Las relaciones permiten predecir el comportamiento de la conductividad eléctrica al variar el porcentaje de cada uno de los elementos que la componen. Las ecuaciones indican que debe suministrarse una relación 3:100, 3 de fertilizante por 100 de agua para obtener la conductividad en el rango de 2 a 3 mS/cm, valor donde la planta tiene un mejor aprovechamiento de los nutrientes. Al comparar la caída de presión en la salida de la inyección el sistema Venturi pierde 40 kPa y el equipo con bombas de paletas no presenta pérdidas de presión.

  20. Bomba za pazuhhoi

    Index Scriptorium Estoniae

    2006-01-01

    Nn tuuma klubi liikmed USA, Venemaa (NSVL järglane), Suurbritannia, Prantsusmaa ja Hiina, tuumarelva leviku piiramise leping, strateegiliste ja taktikaliste tuumalõhkepeade omanikud, riigid, kus tegeldakse tuumarelvade väljatöötamisega

  1. Kossovskaja bomba / Ruslan Gorevoi

    Index Scriptorium Estoniae

    Gorevoi, Ruslan

    2006-01-01

    Pärast Nõukogude Liidu lagunemist tekkinud tunnustamata vabariikidel võib tekkida võimalus legaliseerida end rahvusvahelisel tasandil, Kosovo iseseisvumisele järgnevatest võimalikest protsessidest

  2. Kossovskaja bomba / Ruslan Gorevoi

    Index Scriptorium Estoniae

    Gorevoi, Ruslan

    2006-01-01

    Pärast Nõukogude Liidu lagunemist tekkinud tunnustamata vabariikidel võib tekkida võimalus legaliseerida end rahvusvahelisel tasandil, Kosovo iseseisvumisele järgnevatest võimalikest protsessidest

  3. Bomba za pazuhhoi

    Index Scriptorium Estoniae

    2006-01-01

    Nn tuuma klubi liikmed USA, Venemaa (NSVL järglane), Suurbritannia, Prantsusmaa ja Hiina, tuumarelva leviku piiramise leping, strateegiliste ja taktikaliste tuumalõhkepeade omanikud, riigid, kus tegeldakse tuumarelvade väljatöötamisega

  4. Size-exclusion chromatography as a linear transfer system: purification of human influenza virus as an example.

    Science.gov (United States)

    Kalbfuss, Bernd; Flockerzi, Dietrich; Seidel-Morgenstern, Andreas; Reichl, Udo

    2008-09-15

    Preparative size-exclusion chromatography suffers from low selectivity and productivity. Empirical optimization of operating conditions constitutes a laborious task due to many parameters. Here, a modeling framework based on linear systems theory is presented for predicting the influence of volume overloading. Impulse-responses characterizing system behavior are derived from experimental data by maximum entropy deconvolution. Theoretical derivations are validated experimentally by study of a model system and chromatography of human influenza virus. By application of the theory it is demonstrated how group separation operations can be optimized with respect to yield, purity, productivity and dilution of the product.

  5. Purification of functionalized DNA origami nanostructures.

    Science.gov (United States)

    Shaw, Alan; Benson, Erik; Högberg, Björn

    2015-05-26

    The high programmability of DNA origami has provided tools for precise manipulation of matter at the nanoscale. This manipulation of matter opens up the possibility to arrange functional elements for a diverse range of applications that utilize the nanometer precision provided by these structures. However, the realization of functionalized DNA origami still suffers from imperfect production methods, in particular in the purification step, where excess material is separated from the desired functionalized DNA origami. In this article we demonstrate and optimize two purification methods that have not previously been applied to DNA origami. In addition, we provide a systematic study comparing the purification efficacy of these and five other commonly used purification methods. Three types of functionalized DNA origami were used as model systems in this study. DNA origami was patterned with either small molecules, antibodies, or larger proteins. With the results of our work we aim to provide a guideline in quality fabrication of various types of functionalized DNA origami and to provide a route for scalable production of these promising tools.

  6. Electron beam silicon purification

    Energy Technology Data Exchange (ETDEWEB)

    Kravtsov, Anatoly [SIA ' ' KEPP EU' ' , Riga (Latvia); Kravtsov, Alexey [' ' KEPP-service' ' Ltd., Moscow (Russian Federation)

    2014-11-15

    Purification of heavily doped electronic grade silicon by evaporation of N-type impurities with electron beam heating was investigated in process with a batch weight up to 50 kilos. Effective temperature of the melt, an indicative parameter suitable for purification process characterization was calculated and appeared to be stable for different load weight processes. Purified material was successfully approbated in standard CZ processes of three different companies. Each company used its standard process and obtained CZ monocrystals applicable for photovoltaic application. These facts enable process to be successfully scaled up to commercial volumes (150-300 kg) and yield solar grade silicon. (copyright 2014 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim) (orig.)

  7. Experience with infrasonic purification systems for DENOX reactor purification at Tiefstack heating power station; Erfahrungen zum Einsatz von Infraschall-Reinigungsanlagen fuer die Abreinigung von DENOX-Reaktoren im HKW Tiefstack

    Energy Technology Data Exchange (ETDEWEB)

    Valckenaere, J.; Basener, H.

    1999-07-01

    Solid fuels, whether brown coal, coal, or refuse-derived fuels, cause ash deposition on the boiler heating surfaces. Common purification methos are soot blowoff, pebble cleaning, chemical or mechanical cleaning and, if nothing succeeds, shutoff and manual cleaning. All these techniques are uneconomical as they result in reduced boiler capacities between blowoff and purification cycles. They also cause wear of the boiler which necessitates costly maintenance and repair. The authors propose a new technology in which the dust or soot particles are kept in motion in order to prevent deposition. Infrasonic techniques generate high-energy waves with higher amplitudes which are necessary for inducing ash particle movement. The sound propagates evenly in all directions and has excellent reflection characteristics. Even otherwise inaccessible parts of the boiler, heat exchanger or catalytic converter are reached, ash deposition is prevented, and the boiler is kept clean. [German] Bei der Energieerzeugung verbrennen feststoffgefeuerte Kraftwerke und Muellverbrennungsanlagen Brennstoffe (Braunkohle, Steinkohle, Muell,...), die Reinigungsprobleme durch Ascheablagerungen auf den Kesselheizflaechen verursachen. Die derzeitige Reinigung im Betrieb erfolgt durch Russblasen, Kugelreinigung, chemische und mechanische Reinigung und wenn das nicht hilft, bleibt meistens nur Abstellen und Handreinigung. Konventionelle Reinigungsmethoden sind unwirtschaftlich, weil der Kesselwirkungsgrad zwischen jedem Blas- oder Reinigungszyklus absinkt. Diese Reinigungsarten verursachen einen Verschleiss im Kessel, so dass hohe Wartungs- und Reparaturkosten anfallen. Insgesamt liegt der Energieverbrauch z.B. beim Russblasen zwischen 1 bis 8% des erzeugten Dampfes je nach Brennstoff und Kessel. Die Kesselbetreiber wuenschen aber durchgaengig saubere Heizflaechen, moeglichst wenig Russblasen, keinen Verschleiss im Kessel, einen hohen Wirkungsgrad und vor allem keine Stillstaende. Das Ziel ist die

  8. Production and purification of staphylococcal nuclease in Lactococcus lactis using a new expression-secretion system and a pH-regulated mini-reactor

    Directory of Open Access Journals (Sweden)

    Duvignau Thomas

    2010-05-01

    Full Text Available Abstract Background Staphylococcal (or micrococcal nuclease or thermonuclease (SNase or Nuc is a naturally-secreted nucleic acid degrading enzyme that participates in Staphylococcus aureus spread in the infected host. Purified Nuc protein can be used as an exogenous reagent to clear cellular extracts and improve protein purification. Here, a recombinant form of Nuc was produced and secreted in a Gram-positive host, Lactococcus lactis, and purified from the culture medium. Results The gene segment corresponding to the S. aureus nuclease without its signal peptide was cloned in an expression-secretion vector. It was then fused to a lactococcal sequence encoding a signal peptide, and expressed under the control of a lactococcal promoter that is inducible by zinc starvation. An L. lactis subsp cremoris model strain (MG1363 transformed with the resulting plasmid was grown in either of two media (GM17v and CDM that are free of animal compounds, allowing GMP (Good Manufacturing Practice production. Induction conditions (concentration of the metal chelator EDTA and timing of addition in small-scale pH-regulated fermentors were optimized using LacMF (Lactis Multi-Fermentor, a home-made parallel fermentation control system able to monitor 12 reactors simultaneously. Large amounts of recombinant Nuc (rNuc were produced and secreted in both media, and rNuc was purified from GM17v medium in a single-step procedure. Conclusions In L. lactis, rNuc production and secretion were optimal after induction by 0.5 mM EDTA in small scale (200 mL GM17v exponential phase cultures (at an OD600 of 2, leading to a maximal protein yield of 210 mg per L of culture medium. Purified rNuc was highly active, displaying a specific activity of 2000 U/mg.

  9. Acoplamiento de la hidráulica de pozos y la teoría de bombas para la determinación de caudales en régimen permanente

    OpenAIRE

    Espert Alemany, Vicent; Baeza Arnau, I.; López Patiño, G.; Martínez Solano, F. Javier

    2000-01-01

    En el presente artículo se estudia el punto de funcionamiento de un campo de pozos que extraen agua de un acuífero cautivo trabajando en régimen permanente. El método de cálculo propuesto es un acoplamiento entre las ecuaciones de la hidráulica de pozos y la teoría de bombas hidráulicas, teniendo en cuenta además las características de la instalación que alimenta cada una de las bombas. Se plantea un sistema de ecuaciones no lineal, a resolver por métodos numéricos, el cual proporciona el ...

  10. Período de recuperação do investimento em bomba hidráulica de melhor rendimento em sistemas de bombeamento na tarifa horossazonal verde

    OpenAIRE

    Zocoler, João L.; Hernandez,Fernando B. T.; Saad,João C. C.; Cruz,Raimundo L.

    2011-01-01

    Neste trabalho, ajustou-se um modelo matemático para quantificar o efeito da variação do rendimento da bomba hidráulica na variação dos custos de um sistema de bombeamento, na estrutura tarifária horossazonal verde (subgrupo A4) e o tempo de recuperação do capital investido no equipamento de maior rendimento. Em seguida, o mesmo foi aplicado a um sistema de bombeamento para suprimento de um sistema de irrigação do tipo pivô central. As opções de rendimento da bomba hidráulica foram: 69,5% (bo...

  11. Sodium Purification Device for Production of Tantalum Powder

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    In the process of tantalum powder production it requires pure sodium to reduce potassium fluotantalate, thus the design of a sodium purification device is improved, later it is built and commissioned. The device includes sodium transportation tank, storage tank, filter, cold trap, final storage tank, metering tank, regulating valve, argon purification system, electric control panel and instrument. Industrial purity sodium is purified, the impurities in the sodium were reduced to very

  12. HOUSEHOLD PURIFICATION OF FLUORIDE CONTAMINATED MAGADI (TRONA)

    DEFF Research Database (Denmark)

    1997-01-01

    Purification of fluoride contaminated magadi is studied using bone char sorption and calcium precipitation. The bone char treatment is found to be workable both in columns and in batches where the magadi is dissolved in water prior to treatment. The concentrations in the solutions were 89 g magadi....../L and 95 and 400 mg F/L respectively in natural and synthetic solutions. The fluoride removal capacities observed were 4.6 mg F/g bone char for the column system and 2.7 mg F/g bone char for the batch system in case of synthetic magadi solution. It is however concluded that the batch system is the best...... treatment method. A procedure for purification of fluoride contaminated magadi at household level is described....

  13. Design and Application of Smoke and Dust Purification System for Steel Pipe External Corrosion Host Head%钢管外防腐主机模头烟尘净化系统的设计与应用

    Institute of Scientific and Technical Information of China (English)

    涂君俊

    2014-01-01

    According to environmental pollution problems caused by smoke and dust which appeared in extrusion and winding process of polyethylene/polypropylene and adhesive, a reliable operation smoke and dust purification system was designed. This system consists of plate filter, draught fan, sound proof box, exit silencer, transducer, pressure sensing system, suction hook suite etc. It emphasized the design and function of suction hook and plate filter. The smoke and dust purification system can solve the smoke purification treatment and harmful pollution problem caused from PE/PP heated.%针对钢管在外防腐工艺中聚乙烯(聚丙烯)及胶粘剂挤出过程和缠绕表面过程所产生的烟尘及对车间环境污染的问题,设计了一种工作可靠的烟尘净化系统。烟尘净化系统由板式过滤器、风机及隔音箱、出口消音器、变频器、压力感应系统及吸气罩套件等组成,重点介绍了该系统的主要部件吸气罩和板式过滤器的设计及功能。烟尘净化系统较好地解决了防腐烟尘净化处理及PE/PP受热产生的有害污染问题。

  14. [Purification of eutrophic wastewater by Cyperus alternifolius, Coleus blumei and Jasminum sambac planted in a floating phytoremediation system].

    Science.gov (United States)

    Liu, Shizhe; Lin, Dongjiao; Tang, Shujun; Luo, Jian

    2004-07-01

    In a greenhouse study, Cyperus alternifolius, Coleus blumei and Jasminum sambac were cultured in a floating phytoremediation system with plantation cups inserted into a polyfoam plate that floated in the upper part of a tank filled with 100 L domestic wastewater. The contents of chemical oxygen demand (CODCr), total P (T-P), total N (T-N), soluble P(S-P), ammonia-nitrogen (NH4+ -N) and nitrate-nitrogen (NO3- -N) in the domestic wastewater were tested during the growth of these three plants. The results showed that Cyperus alternifolius and Coleus blumei could grow well in the floating phytoremediation system, their dry weight being 285.8% and 371.4% of the initial weight of planting, respectively, but Jasminum sambac could not grow well, being 125.0% of the initial weight of planting. The removal rate of TN by these 3 plants was 68.0%, 62.0% and 45.0%, and that of NO3- -N, CODCr and TP was 98.0%, 80.0% and 92.0%, 78.0%, 66.0% and 55.0%, and 90.6%, 90.5% and 88.0% respectively. Cyperus alternifolius and Coleus blumei had good effects on the removal of pollutants in the floating phytoremediation system.

  15. Extraction and purification of anthraquinones derivatives from Aloe vera L. using alcohol/salt aqueous two-phase system.

    Science.gov (United States)

    Tan, Zhi-jian; Li, Fen-fang; Xu, Xue-lei

    2013-08-01

    An alcohol/salt aqueous two-phase system (ATPS) composed of 1-propanol and (NH4)2SO4 was employed to purify anthraquinones (AQs) extracted from Aloe vera L. The main influencing system parameters such as type of alcohol, type and concentration of salt, temperature and pH were investigated in detail. Under the optimal extraction conditions, AQs can be extracted into alcohol-rich phase with high extraction efficiency, meanwhile majority polysaccharides, proteins, mineral substances and other impurities were extracted into salt-rich phase. Partitioning of AQs is dependent on hydrophobic interaction, hydrogen bond interaction, and salting-out effect in ATPS. Temperature also played a great role in the partitioning. After ATPS extraction, alcohol can be recycled by evaporation; moreover, salt can be recycled by dilution crystallization method. Compared with other liquid-liquid extractions, this alcohol/salt system is much simpler, lower in cost with easier recovery of phase-forming components, which has the potential scale-up in down-processing of active ingredients in plant.

  16. Presión de saturación del licor amoniacal y su relación con el fenómeno de la cavitación en bombas centrífugas

    Directory of Open Access Journals (Sweden)

    Jorge L. Reyes-Cruz

    2015-05-01

    Full Text Available Se experimentó con licor amoniacal con el fin de obtener los valores numéricos de la presión de vapor y establecer una relación entre la caída de presión y la formación de burbujas asociadas al fenómeno de la cavitación en bombas centrífugas que trasiegan este fluido, en el cual están presentes sólidos disueltos y en suspensión. La experimentación se llevó a cabo en dos etapas en las que se obtuvo la presión de saturación del licor amoniacal y la caída de presión cuando se induce la cavitación en una bomba centrífuga. Se concluye que la presión de formación de burbujas durante el trasiego del licor amoniacal a una concentración de 27 % es de 45,6.103 Pa a 22,15 ºC de temperatura de saturación. Estos valores constituyen los límites inferiores de presión para que se produzca la cavitación para una abertura de la válvula del 75 % en las bombas centrífugas que trasiegan licor amoniacal.

  17. High-temperature metal purification using a compact, portable rf heating and levitation system on the wake shield

    Science.gov (United States)

    Hahs, C. A.

    1990-01-01

    The potential use of a compact, battery-operated rf levitator and heating system to purify high-temperature melting materials in space is described. The wake shield now being fabricated for the Space Vacuum Epitaxy Center will provide an Ultra-high vacuum (10(exp -14) Torr hydrogen, 10(exp -14) Torr helium, 10(exp -30) Torr oxygen). The use of the wake shield to purify Nb, Ti, W, Ir, and other metals to a purity level not achievable on earth is described.

  18. Expression, Purification, Crystallization and Molecular Replacement Studies of TorI, an Inhibition Protein of Tor System

    Institute of Scientific and Technical Information of China (English)

    HUANG Wei; YUAN Cai; Ansaldi Mireille; Morelli Xavier; Edward J. Meehan; CHEN Li-Qing; HUANG Ming-Dong

    2007-01-01

    TorI, a Tor system inhibitor acting through protein-protein interaction with the TorR response regulator, is an excisionase that interacts with the integrase and DNA during prophage excision. It has been crystallized by the vapor-diffusion method using polyethylene glycol 3350 as a precipitant at pH 8.5. The X-ray diffraction data sets from the TorI crystal was collected at a resolution of 2.1 (A), using a synchrotron source. The crystal belongs to primitive monoclinic lattice with cell parameters of 46.210(A) × 53.992(A) × 73.561(A)

  19. Las masacres del olvido: Napalpí y Rincón Bomba en la genealogía del genocidio y el racismo de estado en la Argentina As massacres esquecidas: Napalpí e Rincón Bomba na genealogia do genocídio e o racismo de Estado em Argentina The massacres of the oblivion: Napalpí and Rincon Bomba in the genealogy of the genocide and racism of state in Argentine

    Directory of Open Access Journals (Sweden)

    Héctor Hugo Trinchero

    2009-06-01

    Full Text Available Resumen El objetivo del artículo es intentar trazar una genealogía de algunas prácticas genocidas en la Argentina y, de esta manera, poder comprender las formas recurrentes de masacres sobre población indígena y no indígena producidas por el Estado nacional argentino en su proceso de formación neo-colonial. Específicamente, se detendrá en dos casos que sucedieron ya bien entrado el siglo pasado, es decir, muy a posteriori de la pretendida solución final del General Roca en el sur y las campañas de exterminio desarrolladas por el ejército argentino en el norte y muy anteriores al genocidio de la última dictadura militar. Se trata de la masacre de Napalpí, llevada a cabo en el año 1924 en la provincia de Chaco, y la masacre denominada "Rincón Bomba", perpetrada en el año 1947 en la provincia de Formosa. Ambas masacres de pueblos originarios están siendo revisadas recientemente como un necesario ejercicio de la verdad y la memoria.O objetivo desse artigo é tentar fazer uma genealogia de algumas praticas genocidas em Argentina e, desse maneira, conseguir compreender as formas repetidas das massacres da população indígena e não indígena provocadas pelo Estado nacional Argentino no processo de formação neo-colonial. Especificamente, se analisarão dois casos que aconteceram bem começado o século passado, ou seja, bem depois da pretendida solução final proposta pelo General Roca no sul do pais e as campanhas de extermínio desenvolvidas pelo exercito Argentino no Norte e muito anteriores ao genocídio da ultima ditadura militar. O artigo é sobre a massacre de Napalpí -acontecida no ano 1924 na Província do Chaco-, e a massacre denominada "Rincón Bomba"- acontecida no ano 1947 na Província de Formosa-. As duas massacres de Povos Originários estão sendo revisadas em tempo recente como um necessário exercício de memória e de verdade.The aim of this article is to draw up a genealogy of certain genocide practices in

  20. [Purification efficiency of vertical-flow wetland system constructed by cinder and turf substrate on municipal wastewater].

    Science.gov (United States)

    Cui, Lihua; Zhu, Xizhen; Luo, Shiming; Liu, Yihu

    2003-04-01

    Vertical-flow constructed wetlands (VFCWs) system not only has a higher hydraulic loading rate (54-64 cm.d-1), but also has a good removal efficiency for organics, ammonia nitrogen (AN) and total phosphorus (TP). The removal efficiencies of COD, BOD5, AN, and TP for septic tank effluent were 76-87%, 82-92%, 75-85% and 77-91%, respectively, and the average effluent concentrations of COD, BOD5, AN, and TP in the treated effluent were less than 60, 20, 25 and 2.0 mg.L-1, respectively. A comparison of planted and unplanted columns showed that plantation of Cyperus alternifolius could increase the removal rates of AN, TN, and TP by 2-3%, 4-6%, and 10-14%, respectively.

  1. Partitioning and purification of extracellular β-1,3-1,4-glucanase in aqueous two-phase systems

    Institute of Scientific and Technical Information of China (English)

    HE Guo-qing; ZHANG Xiu-yan; TANG Xing-jun; CHEN Qi-he; RUAN Hui

    2005-01-01

    The partition behaviors of β-1,3-1,4-glucanase, α-amylase and neutral proteases from clarified and whole fermentation broths of Bacillus subtilis ZJF-1A5 were investigated. An aqueous two-phase system (polyethylene glycol (PEG)/MgSO4)was examined with regard to the effects of PEG molecular weight (MW) and concentration, MgSO4 concentration, pH and NaCl concentration on enzyme partition and extraction. The MW and concentration of PEG were found to have significant effects on enzyme partition and extraction with low MW PEG showing the greatest benefit in the partition and extraction of β-glucanase with the PEG/MgSO4 system. MgSO4 concentration influenced the partition and extraction of β-glucanase significantly. pH had little effect on β-glucanase or proteases partition but affected α-amylase partition when pH was over 7.0. The addition of NaCl had little effect on the partition behavior of β-glucanase but had very significant effects on the partitioning of α-amylase and on the neutral proteases. The partition behaviors of β-glucanase, α-amylase and proteases in whole broth were also investigated and results were similar to those obtained with clarified fermentation broth. A two-step process for purifying β-glucanase was developed, which achieved β-glucanase recovery of 65.3% and specific activity of 14027 U/mg, 6.6 times improvement over the whole broth.

  2. 浅谈医院净化区域智能化系统在工程中的应用%The Applications in the Engineering of the Hospital Purification Region Intelligent Systems

    Institute of Scientific and Technical Information of China (English)

    李静娟; 王晨旭

    2012-01-01

    主要介绍医院净化区域智能化系统的设置,特别是手术部智能化各子系统设置内容,且着重强调了一体化手术室实现的具体功能。%This paper introduces the intelligent system settings in the hospital purification region, especially the intelligent subsystem settings in the Department of operation, and it emphasis on the specific functions for the achieve- ment of the integration of the operating room.

  3. Application Analysis of the Pharmaceutical Purification and Low Temperature Air-conditioning and Refrigeration System%医药净化低温空调制冷系统运行的应用分析

    Institute of Scientific and Technical Information of China (English)

    王巍

    2011-01-01

    This paper introduces the structure and principle of purification and air-conditioning system, and analyzes the cooling and defrosting modes from aspects of technique application and energy saving. Defrosting is the key link of low-temperature purification air-conditioning system. The system mentioned in this paper adopted double air-conditioning boxes. Each box is equipped with separate refrigeration system and is able to cool and defrost alternately, thus to meet the demand of manufacturing technique to the environment.%本文简述了净化制冷空调系统的基本构成与制冷原理.对低温表冷器的供冷与化霜方式,从技术应用和节能方面进行了分析.低温净化空调的制冷系统关键环节是要解决好化霜,通过采用双空调箱,分别各自设有独立的制冷系统,交替制冷或化霜,不问断送风,能解决生产工艺对环境的特殊要求.

  4. A co-beneficial system using aquatic plants: bioethanol production from free-floating aquatic plants used for water purification.

    Science.gov (United States)

    Soda, S; Mishima, D; Inoue, D; Ike, M

    2013-01-01

    A co-beneficial system using constructed wetlands (CWs) planted with aquatic plants is proposed for bioethanol production and nutrient removal from wastewater. The potential for bioethanol production from aquatic plant biomass was experimentally evaluated. Water hyacinth and water lettuce were selected because of their high growth rates and easy harvestability attributable to their free-floating vegetation form. The alkaline/oxidative pretreatment was selected for improving enzymatic hydrolysis of the aquatic plants. Ethanol was produced with yields of 0.14-0.17 g-ethanol/ g-biomass in a simultaneous saccharification and fermentation mode using a recombinant Escherichia coli strain or a typical yeast strain Saccharomyces cerevisiae. Subsequently, the combined benefits of the CWs planted with the aquatic plants for bioethanol production and nutrient removal were theoretically estimated. For treating domestic wastewater at 1,100 m(3)/d, it was inferred that the anoxic-oxic activated sludge process consumes energy at 3,200 MJ/d, whereas the conventional activated sludge process followed by the CW consumes only 1,800 MJ/d with ethanol production at 115 MJ/d.

  5. Purification and in vitro antioxidative effects of giant squid muscle peptides on free radical-mediated oxidative systems.

    Science.gov (United States)

    Rajapakse, Niranjan; Mendis, Eresha; Byun, Hee-Guk; Kim, Se-Kwon

    2005-09-01

    Low molecular weight peptides obtained from ultrafiltration (UF) of giant squid (Dosidicus gigas) muscle protein were studied for their antioxidative effects in different in vitro oxidative systems. The most potent two peptides, Asn-Ala-Asp-Phe-Gly-Leu-Asn-Gly-Leu-Glu-Gly-Leu-Ala (1307 Da) and Asn-Gly-Leu-Glu-Gly-Leu-Lys (747 Da), exhibited their antioxidant potential to act as chain-breaking antioxidants by inhibiting radical-mediated peroxidation of linoleic acid, and their activities were closer to highly active synthetic antioxidant, butylated hydroxytoluene. Addition of these peptides could enhance the viability of cytotoxic embryonic lung fibroblasts significantly (P<.05) at a low concentration of 50 microg/ml, and it was presumed due to the suppression of radical-induced oxidation of membrane lipids. Electron spin trapping studies revealed that the peptides were potent scavengers of free radicals in the order of carbon-centered (IC(50) 396.04 and 304.67 microM), hydroxyl (IC(50) 497.32 and 428.54 microM) and superoxide radicals (IC(50) 669.34 and 573.83 microM). Even though the exact molecular mechanism for scavenging of free radicals was unclear, unusually high hydrophobic amino acid composition (more than 75%) of giant squid muscle peptides was presumed to be involved in the observed activities.

  6. Removal of Pb, Cd, and Cr in a water purification system using modified mineral waste materials and activated carbon derived from waste materials

    Science.gov (United States)

    Lu, H. R.; Su, L. C.; Ruan, H. D.

    2016-08-01

    This study attempts to find out and optimize the removal efficiency of heavy metals in a water purification unit using a low-cost waste material and modified mineral waste materials (MMWM) accompanied with activated carbon (AC) derived from waste materials. The factors of the inner diameter of the purification unit (2.6-5cm), the height of the packing materials (5-20cm), the size of AC (200-20mesh), the size of MMWM (1-0.045mm), and the ratio between AC and MMWM in the packing materials (1:0 - 0:1) were examined based on a L18 (5) 3 orthogonal array design. In order to achieve an optimally maximum removal efficiency, the factors of the inner diameter of the purification unit (2.6-7.5cm), the height of the packing materials (10-30cm), and the ratio between AC and MMWM in the packing materials (1:4-4:1) were examined based on a L16 (4) 3 orthogonal array design. A height of 25cm, inner diameter of 5cm, ratio between AC and MMWM of 3:2 with size of 60-40mesh and 0.075-0.045mm, respectively, were the best conditions determined by the ICP-OES analysis to perform the adsorption of heavy metals in this study.

  7. Custo de bombas centrífugas funcionando como turbinas em microcentrais hidrelétricas Cost of pumps as hydraulic turbines for micro-scale hydropower

    Directory of Open Access Journals (Sweden)

    Carlos R. Balarim

    2004-04-01

    Full Text Available A implantação de microcentrais hidrelétricas é uma das alternativas para suprir com energia comunidades pequenas e isoladas, situadas normalmente na área rural. O aproveitamento de potenciais hidráulicos de pequeno porte é uma alternativa cada vez mais viável devido não só à falta de recursos financeiros para os grandes empreendimentos, mas também pelo imenso potencial de geração em centrais de pequeno porte, que pouco tem sido aproveitado. O objetivo deste trabalho foi de apresentar uma metodologia simples de engenharia para estimar o custo das Bombas Funcionando como Turbinas (BFTs utilizadas em microcentrais hidrelétricas, que possam ser usadas em estudos preliminares de novos aproveitamentos hidrelétricos, sem uma investigação detalhada dos lugares onde se pretende implantar. Os custos foram obtidos consultando-se diretamente os fabricantes de equipamentos e o mercado da praça de Ponta Grossa - PR. Os resultados mostraram que, para as microcentrais hidrelétricas, sempre que os custos constituírem o aspecto dominante, e para potências até 50 kW, a opção por Bombas Funcionando como Turbinas (BFTs deve ser considerada em lugar das turbinas hidráulicas.The implantation of micro hydroelectric power stations is one of the alternatives to supply energy to small and isolated communities, normally located in rural areas. The use of small load hydraulic potential is an alternative more feasible nowadays, not only because it lacks financial resources for big undertakings, but also, due to the small hydro power station hydraulic generation potential, which just a very small part has been taken advantage of. The objective of this study was to show a simple engineering methodology in order to estimate the electro-mechanic (generating set equipment costs used in micro hydroelectric power plants, which can be applied on preliminary studies about new hydroelectric power stations to be built, without detailed investigation about the

  8. Determinación de los parámetros de funcionamiento y recurso residual medio de una bomba hidráulica

    Directory of Open Access Journals (Sweden)

    Luciano Pérez Sobrevilla

    2004-01-01

    Full Text Available Como parte de las in ves tig acio nes que se llev an a cabo en la Un iversidad Autónoma Ch apingo de México , en el Departamen to de Ingeniería Mecánica Agrícola (DIMA y en la Univ ersid ad Agraria de La Habana, en el Centro de Mecanización Agropecuaria (CEMA, dirigidas al desarrollo d e la Oleohidráulica, el trabajo tuvo como objetiv o, evaluar un a bomb a hidráulica de engranajes de desplazamientos 32 cm3 con aceite Elf Dis al HD 4/40 PLUS. El experimento fue desarrollado en el lab oratorio d e oleohidráulica del CEMA. La meto dología contempló la u tilización de un a instalación experimen tal con un banco de prueba de elementos hidráulicos. Fue utilizado un diseño cuadrado latino , en cuatro perío dos (tratamien tos diferentes (T1 - al inicio d el ensay o; T2 - a las 1 5 ho ras ; T3 – a las 30 horas y T4 - a las 45 horas d e trabajo , con cuatro mediciones (filas en cuatro días d iferentes (columnas. En cada unidad experimental fueron estudiados el torque, la potencia, la eficiencia volumétrica, eficiencia mecán ica, eficiencia total, co eficiente de isogo nalidad, coeficiente d e fugas y coeficien te de rozamiento líquido a 80 b ar de presión. Se determinó el desgaste de los elementos de la bomba hid ráulica al final del ensayo, utilizando el recurso residual a través de una serie de medicion es de control, por la dismin ución del caud al de la misma. En los análisis de varianza respectivo s se pud o apreciar que no hubo d iferencias s ignificativas por filas ni columnas . Sin embargo la diferencia entre tratamiento s fue significativa. Lo s resultados de la in ves tigación aparecen tab ulados en el cuerpo del trabajo. El recurso residual fue de 1 03 3,01 h, in dicand o q ue la vid a útil de esa bomba, trabajand o con aceite co ntaminad o, s erá aproximadamente d e 1 09 3 h, eq uivalentes a 137 jornadas de 8 h de trabajo co ntinuo.

  9. Precisión en flujo de infusión programado de bombas de nutrición enteral Precision in programmed infusion flow of enteral nutrition pumps

    Directory of Open Access Journals (Sweden)

    Juan Ignacio Padilla-Cuadra

    2008-09-01

    Full Text Available Justificación y objetivo: Se evaluó in vitro la precisión en el flujo de nutrición enteral en bombas peristálticas de nutrición enteral con el fin de determinar si ésta puede ser una variable que cause errores en la administración de la fórmula. Materiales y Métodos: Se evaluaron 13 bombas de infusión enteral del tipo peristáltico mediante la aplicación de un modelo de laboratorio que simulaba la administración a un paciente. Se usó para todos los casos una fórmula estándar diluida de la manera recomendada por el fabricante. Con un volumen meta de 240 mL. Se programó en cada bomba un flujo de infusión de 40 mL por hora. Para cada caso según el tiempo de infusión, se calculó el flujo de infusión real y a partir de la diferencia se calculó el porcentaje de error. Resultados: En 12 de las 13 bombas se detectó un error por exceso que fue de 7.5% hasta 52.5%. Solo una bomba, de marca distinta a todas las demás demostró un error de 0 mL/h con respecto a lo programado. Ninguna bomba mostró un déficit en el volumen de infusión. Conclusión: En la mayoría de las bombas evaluadas se detectó un porcentaje de error por exceso que supera lo estipulado por el fabricante.Justification and aim: To evaluate in vitro the accuracy of volume infusion of peristaltic enteral nutrition pumps to determine if this variable may be responsible for error’s in the administration of nutrition support. Materials and Methods: Thirteen pumps were evaluated through simulation using a model that resembles the administration of enteral nutrition to a patient. For every case, we used an standard formula diluted as recommended by the manufacturer. A volume of 240 mL was programmed to be infused at 40 ml per hour. For each case, time was measured until finishing the volume, comparing to the programmed time and calculating the delivery error. Results: In 12 of 13 pumps, we detected error by excess, which varied from 7.5% to 52.5%. Only one pump showed

  10. 血液净化专科护士胜任力指标体系的构建%Construction of competency index system of blood purification specialist nurses

    Institute of Scientific and Technical Information of China (English)

    刘晓鑫; 李乐之

    2016-01-01

    Objective:To construct a competency index system for blood purification specialist nurses and to pro-vide basis for selection,training,performance evaluation,management of specialist nurses.Methods:Semi struc-tured depth interview method and critical incident method were adopted to interview 12 blood purification ex-perts and 12 blood purification specialist nurses in Hunan province,All the data were collected and analyzed by NVivo QSR 8 Chinese version software.Results:Competency index system for blood purification specialist nur-ses included professional knowledge,professional skills,professional ability,personality and self concept.And the 5 dimensions were divided into 18 items.There were some differences in the cognition of the competency of nurses in different position of blood purification.Conclusions:When selecting and training of blood purification specialist nurses,in addition to consider their professional knowledge and skills,one should also pay attention to test and develop their teaching ability,service consciousness,protection,inner concentration spirit,empathy and dedication spirit,and so on,so as to achieve a comprehensive and scientific evaluation.%[目的]构建血液净化专科护士胜任力指标体系,为专科护士选拔、培训、绩效考核、管理等提供依据。[方法]采用半结构式深度访谈法、关键事件法对湖南省12名血液净化专家和12名血液净化专科护士进行访谈收集资料,资料分析采用 QSR NVivo 8.0中文版软件。[结果]血液净化专科护士胜任指标体系包含专业知识、专业技术、专业能力、人格特质及自我概念5个维度(18个项目);不同岗位受访者对血液净化专科护士胜任力特征的认知存在一定差异。[结论]在选聘及培训血液净化专科护士时,除其专业知识技能方面外,还应注重考查和培养其服务意识、防护能力、慎独精神、同理心、敬业精神等,以达到全面、科学地测评。

  11. Papel da MRP1/bomba GS-X na regulação do potencial redox celular, e a influência do estado redox celular na expressão e atividade da MRP1/bomba GS-X

    OpenAIRE

    2005-01-01

    Uma das complicações que levam o paciente terminal de câncer à morte é a imunossupressão. Por sua vez, a superprodução de prostaglandinas ciclopentenônicas (CP-PGs) no plasma desses indivíduos é um fator de risco para depressão imunológica já que as CP PGs bloqueiam inúmeras interações entre células imunológicas. Estudos de nosso grupo revelaram que células tumorais apresentam alta atividade da ATPase bomba GS X/MRP que exporta conjugados sulfidrila, inclusive CP PG na forma de S-conjugados d...

  12. [Extraction and purification method of rice DNA from rice powder containing Konjak flour].

    Science.gov (United States)

    Minematsu, Kazuhiko; Nakamura, Kosuke; Akiyama, Hiroshi; Harikai, Naoki; Nakajima, Osamu; Kitta, Kazumi; Teshima, Reiko; Iizuka, Tayoshi

    2010-01-01

    Rice powder containing Konjak flour made with tuberous roots of Amorphophallus konjac is imported as a rice-processed product from China to Japan. An improved DNA purification method for the polymerase chain reaction (PCR) analysis of rice in such products is necessary, since Konjak flour constituents absorb the DNA purification buffer to form a gel, and cause problems in the subsequent purification steps. Here, we present a simple preparative system for isolation of the rice and a purification method of the rice DNA from the product. The purified DNA was confirmed to be a good template for both PCR and real-time PCR.

  13. Purification of GST-Tagged Proteins.

    Science.gov (United States)

    Schäfer, Frank; Seip, Nicole; Maertens, Barbara; Block, Helena; Kubicek, Jan

    2015-01-01

    This protocol describes the purification of recombinant proteins fused to glutathione S-transferase (GST, GST-tagged proteins) by Glutathione Affinity purification. The GST tag frequently increases the solubility of the fused protein of interest and thus enables its purification and subsequent functional characterization. The GST-tagged protein specifically binds to glutathione immobilized to a matrix (e.g., agarose) and can be easily separated from a cell lysate by a bind-wash-elute procedure. GST-tagged proteins are often used to study protein-protein interactions, again making use of glutathione affinity in a procedure called a GST pull-down assay. The protocol is designed to process 200 ml of E. coli culture expressing intermediate to high amounts of a GST-tagged protein (~25 mg l(-1)). Depending on the expression rate or the available culture volume, the scale can be increased or decreased linearly. The protocol can also be used to purify GST-tagged proteins from other expression systems, such as insect or mammalian cells. Tips are provided to aid in modifying certain steps if proteins shall be recovered from alternative expression systems.

  14. Purification of a-galactosidase from seeds of Sesbania marginata

    Directory of Open Access Journals (Sweden)

    Falco A.L.P.

    2000-01-01

    Full Text Available Alpha-galactosidase taken from a raw extract of Sesbania marginata legume seeds was purified by partitioning in aqueous two-phase systems (ATPS. Initially, galactomannan/dextran 2,000,000 systems were used for the purification, and the partition coefficients of alpha -galactosidase varied from 1.5 to 4.0. However, mass transport in these systems was poor due to the high viscosity of the employed polymers. Therefore, partitioning in polyethyleneglycol (PEG/ sodium phosphate systems and the effect of sodium chloride upon the enzyme purification and the yield of alpha -galactosidase were also investigated. The purification achieved in a single-step was 5.7 with a recovery of 144% of alpha -galactosidase, possibly due to the removal of materials which inhibited alpha -galactosidase activity before the purification. The removal of the main protein contaminants and the highest yields were achieved in PEG 4,000/ sodium phosphate + 6% NaCl system at pH 5.0. Further purification by preparative on-exchange chromatography was also developed.

  15. Logic control of molecular sieve purification system of 65000m3· h-1 air separation plant%65000m3·h-1空分设备分子筛纯化系统逻辑控制

    Institute of Scientific and Technical Information of China (English)

    朱玉芹

    2012-01-01

    The structure and technical process of molecular sieve purification system of 65000m3/ an air separation plant of Hebi coal and electeicity 600 ktpa methanol project are briefed, automatic valve closing / opening logical control of molecular sieve purification system, automatic / manual operation sequence control program,implementation of the sequence control program and optimized reform of the sequence control configuration program of the molecular sieve absorber in accordance with site operation conditions are described.%简介鹤壁煤电股份有限公司化工分公司空分厂65000m3·h-1空分设备分子筛纯化系统结构和工艺流程,介绍分子筛纯化系统阀门自动开关控制逻辑、顺控程序自动/手动运行、顺控程序执行过程,以及根据现场生产运行实际情况,对分子筛吸附器顺控组态程序进行的优化改进.

  16. Online Oxide Contamination Measurement and Purification Demonstration

    Science.gov (United States)

    Bradley, D. E.; Godfroy, T. J.; Webster, K. L.; Garber, A. E.; Polzin, K. A.; Childers, D. J.

    2011-01-01

    Liquid metal sodium-potassium (NaK) has advantageous thermodynamic properties indicating its use as a fission reactor coolant for a surface (lunar, martian) power system. A major area of concern for fission reactor cooling systems is system corrosion due to oxygen contaminants at the high operating temperatures experienced. A small-scale, approximately 4-L capacity, simulated fission reactor cooling system employing NaK as a coolant was fabricated and tested with the goal of demonstrating a noninvasive oxygen detection and purification system. In order to generate prototypical conditions in the simulated cooling system, several system components were designed, fabricated, and tested. These major components were a fully-sealed, magnetically-coupled mechanical NaK pump, a graphite element heated reservoir, a plugging indicator system, and a cold trap. All system components were successfully demonstrated at a maximum system flow rate of approximately 150 cc/s at temperatures up to 550 C. Coolant purification was accomplished using a cold trap before and after plugging operations which showed a relative reduction in oxygen content.

  17. "Bomba hormonal": os riscos da contracepção de emergência na perspectiva dos balconistas de farmácias no Rio de Janeiro, Brasil

    Directory of Open Access Journals (Sweden)

    Elaine Reis Brandão

    Full Text Available Resumo: A pesquisa objetivou conhecer a perspectiva dos balconistas de farmácias sobre a contracepção de emergência na Região Metropolitana do Rio de Janeiro, Brasil. O material empírico advém de pesquisa socioantropológica com vinte entrevistas semiestruturadas com balconistas dos sexos feminino (8 e masculino (12. Os entrevistados apresentam concepções negativas sobre a contracepção de emergência, enfatizando os riscos que ela pode provocar à saúde. O medicamento é considerado uma "bomba hormonal" que pode causar danos aos órgãos reprodutivos femininos e outros sistemas do corpo. Eles destacam os riscos do uso "descontrolado" ou "indiscriminado", especialmente por adolescentes e mulheres jovens. Por ser considerado "perigoso" aos corpos femininos, eles atribuem a responsabilidade de orientação e aconselhamento sobre o uso do método aos médicos ginecologistas e não aos farmacêuticos. Discute-se a necessidade de ampliação do debate público sobre contracepção de emergência no Brasil, incluindo-se os farmacêuticos e balconistas de farmácia, além dos profissionais de saúde e educadores.

  18. Rotating Reverse-Osmosis for Water Purification

    Science.gov (United States)

    Lueptow, RIchard M.

    2004-01-01

    A new design for a water-filtering device combines rotating filtration with reverse osmosis to create a rotating reverse- osmosis system. Rotating filtration has been used for separating plasma from whole blood, while reverse osmosis has been used in purification of water and in some chemical processes. Reverse- osmosis membranes are vulnerable to concentration polarization a type of fouling in which the chemicals meant not to pass through the reverse-osmosis membranes accumulate very near the surfaces of the membranes. The combination of rotating filtration and reverse osmosis is intended to prevent concentration polarization and thereby increase the desired flux of filtered water while decreasing the likelihood of passage of undesired chemical species through the filter. Devices based on this concept could be useful in a variety of commercial applications, including purification and desalination of drinking water, purification of pharmaceutical process water, treatment of household and industrial wastewater, and treatment of industrial process water. A rotating filter consists of a cylindrical porous microfilter rotating within a stationary concentric cylindrical outer shell (see figure). The aqueous suspension enters one end of the annulus between the inner and outer cylinders. Filtrate passes through the rotating cylindrical microfilter and is removed via a hollow shaft. The concentrated suspension is removed at the end of the annulus opposite the end where the suspension entered.

  19. Blood purification and hemo- perfusion

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    The method of blood purification is a new overlapping frontierdiscipline which develops quickly in recent years. It helps overcoming many serious and complicated diseases, even including some incurable illnesses.

  20. Nanomechanical Water Purification Device Project

    Data.gov (United States)

    National Aeronautics and Space Administration — Seldon Laboratories, LLC, proposes a lightweight, low-pressure water purification device that harnesses the unique properties of carbon nanotubes and will operate...

  1. 混合反胶团萃取α-淀粉酶%Purification of α-Amylase Using CTAB/Tween-60 Mixed Reverse Micellar System

    Institute of Scientific and Technical Information of China (English)

    高树刚; 宋伟明; 安红

    2011-01-01

    Reverse micellar system consisting of the surfactants cetyltrimethyl ammonium bromide(CTAB) and Tween-60 in dissolved in n-butanol-isooctane mixture was used for α-amylase purification by reversed micellar extraction.Extraction efficiency of α-amylase was investigated under different extraction conditions using a reverse micellar phase consisting of 4 g/L(CTAB +Tween-60,2.0:1.0,n/n) in n-butanol-isooctane mixture(1.0:1.0,V/V) and an aqueous phase made up of crude α-amylase solution containing 0.04 mol/L NaCl.The extraction efficiency of α-amylase was 91% when the extraction temperature,ratio of organic phase to aqueous phase and oscillation time were 40 ℃,2.0:1.0 and 10 min,respectively.The reverse extraction efficiency of α-amylase was 65% after 10 min oscillation at 50 ℃ when the aqueous phase was comprised of aqueous phase and organic phase at a ratio of 1.0:2.0(V/V) at pH 4.5 containing 2.5 mol/L NaCl.The reverse micelles could be repeatedly used and the extraction efficiency of α-amylase at an aqueous phase-to-organic phase ratio of 1.0:2.75 remained 71% in the second use.%以十六烷基三甲基溴化铵(CTAB)和脱水山梨醇单硬脂酸酯聚氧乙烯醚(Tween-60)为混合表面活性剂溶于正丁醇-异辛烷中构成反胶团系统,萃取纯化α-淀粉酶。研究不同萃取条件下,α-淀粉酶的萃取率。其中反胶团相组成为:ρ(CTAB+Tween-60)=4g/L;n(CTAB):n(Tween-60)=2.0:1.0;V(正丁醇):V(异辛烷)=1.0:1.0。水相组成为:α-淀粉酶配制的粗酶液,此时c(NaCl)=0.04mol/L,水相pH11.04。结果表明:萃取温度40℃、V(有机相):V(水相)=2.0:1.0、振荡时间10min时,α-淀粉酶萃取率可达91%;反萃取水相组成为c(NaCl)=2.5mol/L、pH4.5、V(水相):V(有机相)=1.0:2.0,反萃取振荡时间10min、温度50℃时,α-淀粉酶反萃取率可达65%。反胶团相可重复使用,当V

  2. Fundamental limitations in the purifications of tensor networks

    Energy Technology Data Exchange (ETDEWEB)

    De las Cuevas, G.; Cirac, J. I. [Max Planck Institute for Quantum Optics, Hans-Kopfermann-Str. 1, 85748 Garching (Germany); Cubitt, T. S. [Department of Computer Science, University College London, London WC1E 6BT, United Kingdom and DAMTP, Centre for Mathematical Sciences, University of Cambridge, Wilberforce Road, Cambridge CB3 0WA (United Kingdom); Wolf, M. M. [Zentrum Mathematik, Technische Universität München, 85748 Garching (Germany); Pérez-García, D. [Departamento de Análisis Matemático and IMI, Universidad Complutense de Madrid, 28040 Madrid, Spain and ICMAT, C/ Nicolás Cabrera, Campus de Cantoblanco, 28049 Madrid (Spain)

    2016-07-15

    We show a fundamental limitation in the description of quantum many-body mixed states with tensor networks in purification form. Namely, we show that there exist mixed states which can be represented as a translationally invariant (TI) matrix product density operator valid for all system sizes, but for which there does not exist a TI purification valid for all system sizes. The proof is based on an undecidable problem and on the uniqueness of canonical forms of matrix product states. The result also holds for classical states.

  3. Fundamental limitations in the purifications of tensor networks

    Science.gov (United States)

    De las Cuevas, G.; Cubitt, T. S.; Cirac, J. I.; Wolf, M. M.; Pérez-García, D.

    2016-07-01

    We show a fundamental limitation in the description of quantum many-body mixed states with tensor networks in purification form. Namely, we show that there exist mixed states which can be represented as a translationally invariant (TI) matrix product density operator valid for all system sizes, but for which there does not exist a TI purification valid for all system sizes. The proof is based on an undecidable problem and on the uniqueness of canonical forms of matrix product states. The result also holds for classical states.

  4. Partition Efficiency of High-Pitch Locular Multilayer Coil for Countercurrent Chromatographic Separation of Proteins Using Small-Scale Cross-Axis Coil Planet Centrifuge and Application to Purification of Various Collagenases with Aqueous-Aqueous Polymer Phase Systems.

    Science.gov (United States)

    Shinomiya, Kazufusa; Kobayashi, Hiroko; Inokuchi, Norio; Nakagomi, Kazuya; Ito, Yoichiro

    2011-01-01

    Partition efficiency of the high-pitch locular multilayer coil was evaluated in countercurrent chromatographic (CCC) separation of proteins with an aqueous-aqueous polymer phase system using the small-scale cross-axis coil planet centrifuge (X-axis CPC) fabricated in our laboratory. The separation column was specially made by high-pitch (ca 5 cm) winding of 1.0 mm I.D., 2.0 mm O.D. locular tubing compressed at 2 cm intervals with a total capacity of 29.5 mL. The protein separation was performed using a set of stable proteins including cytochrome C, myoglobin, and lysozyme with the 12.5% (w/w) polyethylene glycol (PEG) 1000 and 12.5% (w/w) dibasic potassium phosphate system (pH 9.2) under 1000 rpm of column revolution. This high-pitch locular tubing yielded substantially increased stationary phase retention than the normal locular tubing for both lower and upper mobile phases. In order to demonstrate the capability of the high-pitch locular tubing, the purification of collagenase from the crude commercial sample was carried out using an aqueous-aqueous polymer phase system. Using the 16.0% (w/w) PEG 1000 - 6.3% (w/w) dibasic potassium phosphate - 6.3% (w/w) monobasic potassium phosphate system (pH 6.6), collagenase I, II, V and X derived from Clostridium hystolyticum were separated from other proteins and colored small molecular weight compounds present in the crude commercial sample, while collagenase N-2 and S-1 from Streptomyces parvulus subsp. citrinus were eluted with impurities at the solvent front with the upper phase. The collagenase from C. hystolyticum retained its enzymatic activity in the purified fractions. The overall results demonstrated that the high-pitch locular multilayer coil is effectively used for the CCC purification of bioactive compounds without loss of their enzymatic activities.

  5. Otimização térmica e econômica de bomba de calor para aquecimento de água, utilizando programação quadrática sequencial e simulação através do método de substituição Newton Raphson

    OpenAIRE

    Obed Alexander Córdova Lobatón

    2011-01-01

    Resumo: O trabalho apresenta metodologias computacionais de otimização e simulação para o desenvolvimento do projeto de um sistema de bomba de calor para aquecimento de água em prédios residenciais, especialmente para uso em banho na região de Campinas. O sistema bomba de calor foi simulado focando o estudo nos trocadores de calor (evaporador e condensador). Cada componente foi otimizado mediante uma análise térmica e econômica que permitiu reduzir custos de investimento e de operação através...

  6. Analysis and treatment of trouble of molecular sieve purification system of 21000 m^3/h air separation plant%21000m^3/h空分设备分子筛纯化系统故障分析及处理

    Institute of Scientific and Technical Information of China (English)

    胥波; 闫伟东; 孔繁魁; 沈荣; 田现德

    2012-01-01

    In the air separation plant with molecular sieve adsorption and purification process, the work condition of molecular sieve purification system restricts the safe and stable run of the entire air separation system. For the troubles frequently occurring in molecular sieve purification system, such as mechanical trouble of switching over valve, valve action-generated program problem, and program-interlocked control of molecular sieve purification system, the judgment of the trouble causes and treatment measures are detailed.%分子筛吸附净化流程空分设备中,分子筛纯化系统的运行工况制约了整个空分系统的安全稳定运行。针对分子筛纯化系统经常出现的切换阀机械故障、阀门动作导致的程序问题以及分子筛纯化系统程序联锁控制等问题,详细介绍了故障原因判断和处理措施。

  7. Estudio del fenómeno de interacción rotor estator (RSI) en una bomba radial, trabajando fuera de las condiciones de diseño óptimo

    OpenAIRE

    Coussirat Núñez, Miguel Gustavo; Fontanals García, Alfred; Panella, L.; Guardo Zabaleta, Alfredo de Jesús

    2014-01-01

    El flujo en el interior de bombas radiales de altas prestaciones es difícil de describir, (geometría compleja y variable, flujo turbulento y no estacionario). La geometría compleja implica partes tanto móviles (rotor) como fijas (estator) con álabes, separadas por un huelgo pequeño que minimiza dimensiones de la máquina y que intenta optimizar la energía entregada al flujo. Al girar la máquina, su geometría varía, cambiando las condiciones del flujo en su interior. El flujo abandona el rotor ...

  8. Desempenho de bomba centrífuga operando com água residuária do processamento do café Centrifugal pump performance using coffee process wastewater

    Directory of Open Access Journals (Sweden)

    Jacinto A. Carvalho

    2008-03-01

    Full Text Available Este trabalho foi desenvolvido com o objetivo de avaliar o desempenho de bomba centrífuga no recalque de águas residuárias provenientes do processamento do café, especificamente do descascamento e da demucilagem. Montou-se uma bancada, composta por caixas d'água, tubulações, válvulas, medidores de vazão e de pressão, e uma motobomba para a realização dos ensaios. Esses consistiram em aberturas parciais do registro, na saída da bomba, e medições da vazão e da pressão desenvolvida, e da energia consumida. Tais procedimentos foram realizados, primeiro, utilizando água pura, e depois com águas residuárias, em suas concentrações originais e com diversos graus de diluições. Os ensaios mostraram quedas acentuadas de altura manométrica e de rendimento, e aumento da potência demandada com a vazão recalcada de água residuária em comparação ao desempenho da mesma bomba operando com água limpa; a diluição das águas residuárias promoveu melhor desempenho da bomba em relação àquela com concentração original.This work was carried out to evaluate the performance of a centrifugal pump operating with wastewater from coffee process. Under laboratory conditions, it was determined the hydraulic characteristics (manometric height, engine power and efficiency for a centrifugal pump, operating with clear water and coffee process wastewater. The results have show that manometric height and efficiency was smaller and the engine power was higher when the coffee process wastewater was used in comparison with results obtained for clean water. The smaller the concentration of that coffee wastewater produced the better the performance of the pump in comparison with the performance obtained with original concentration of the coffee wastewater.

  9. Cinética de secado de fruta bomba (Carica papaya L., cv. Maradol Roja) mediante los métodos de deshidratación osmótica y por flujo de aire caliente

    OpenAIRE

    Daybelis Fernández Valdés; Sahylin Muñiz Becerá; Annia García Pereira; Rafael Cervantes Beyra; Dayvis Fernández Valdés

    2015-01-01

    La deshidratación es uno de los métodos más utilizados para la conservación de frutas y vegetales, mediante su uso se logra extender los períodos de almacenamiento preservando siempre la calidad de los productos. El presente trabajo tiene como objetivo evaluar el comportamiento cinético de las principales propiedades de la fruta bomba (Carica papaya L, cv. Maradol Roja) durante los procesos agroindustriales de deshidratación osmótica (DO) y por flujo de aire caliente (DAC). Las frutas se cort...

  10. Protocol for Initial Purification of Bacteriocin

    Science.gov (United States)

    2015-10-01

    lysate/extract preparation , column purification, and a desalting. The peptide was tracked throughout the process using a soft agar overlay activity... PREPARATION SOLUBLE EXTRACTS COLUMN CHROMATOGRAPHY BACTERIA PURIFICATION CHARACTERIZATION...3  6.1  Preparation of Target Bacteria

  11. Analgesia invasiva domiciliaria en el manejo del dolor postoperatorio en cirugía mayor ambulatoria mediante bombas elastoméricas intravenosas Home invasive analgesia in the management of postoperative pain alter outpatient major surgery using intravenous elastomeric pumps

    Directory of Open Access Journals (Sweden)

    R. Rodríguez de la Torre

    2011-06-01

    Full Text Available Introducción: el dolor postoperatorio moderado-severo sigue siendo un problema en cirugía ambulatoria, ya que provoca problemas de flujo de pacientes, retrasando el alta de los pacientes, siendo uno de los principales motivos de reingreso en los hospitales, y por tanto un importante indicador de calidad de estas Unidades. El empleo de técnicas analgésicas invasivas domiciliarias, en todos sus regímenes, puede controlar el dolor postoperatorio en estas intervenciones y permitir incluirlas en los programas de cirugía ambulatoria. Objetivos: el objetivo de nuestro estudio es valorar la viabilidad y la seguridad de la utilización de bombas de perfusión continua elastoméricas para la administración de analgesia endovenosa continua domiciliaria, a la vez que valorar la eficacia analgésica y el grado de satisfacción de los pacientes intervenidos en régimen ambulatorio. Material y métodos: estudio retrospectivo de 463 pacientes. Una vez intervenidos bajo estrategia de analgesia multimodal, se les coloca dos tipos diferentes de bombas elastoméricas endovenosas (elastómero de dexketoprofeno o de metamizol. La intensidad del dolor, para evaluar la necesidad de analgesia de rescate, se cuantifica con la escala visual analógica o con la escala verbal simple. En el domicilio (24 horas tras la cirugía, la Unidad de Atención Domiciliaria revisa los efectos secundarios, alteraciones del sueño, intensidad del dolor, necesidad de analgesia de rescate y grado de satisfacción. Resultados: un 69% de los pacientes presentaron dolor de carácter leve o ausencia de dolor tras la intervención y únicamente 16 de los 463 pacientes presentaron dolor de carácter severo. El 27% de los pacientes necesitaron analgesia de rescate y un 9% de los pacientes presentaron efectos secundarios atribuibles a los fármacos analgésicos (4% vómitos, 2% mareos, 2,5% somnolencia y 0,5% insomnio. Ningún paciente tuvo que ser reingresado después del alta. Un 83% de

  12. Sensibilidad de la biopsia gástrica en la detección de Helicobacter pylori en pacientes en tratamiento con inhibidores de la bomba de protones.

    Directory of Open Access Journals (Sweden)

    Daisy Azaña

    2012-03-01

    Full Text Available Objetivo: Comparar la sensibilidad de la biopsia gástrica en la detección de Helicobacter pylori (Hp en pacientes que recibieron inhibidores de la bomba de protones (IBP entre 7 y 14 días previos a la endoscopía digestiva alta y los que recibieron medicación diferente a IBP o no usaron medicación. Material y métodos: Estudio tipo serie de casos comparativo. Se incluyeron pacientes con dispepsia sometidos a endoscopía digestiva alta y biopsia gástrica en el Servicio de Gastroenterología del Hospital Nacional Cayetano Heredia entre noviembre 2008 y enero 2009, que habían recibido IBP entre 7 y 14 días. Se excluyeron pacientes con cáncer gástrico, cirugía previa, que se encontraban en terapia antibiótica o con salicilato de bismuto en las 4 semanas previas. Se tomaron dos biopsias en cuerpo y dos en antro. Se utilizó el programa STATA 10 para el análisis. Resultados: Se estudiaron 80 pacientes; 38 (47,5% recibieron IBP. El 64,29% (27/42 de los que no tomaron IBP fueron Hp (+ y el 65,79% (23/38 de los que tomaron IBP (p=0,90. Conclusiones: La proporción de resultados positivos para Hp fue similar en los pacientes que tomaron IBP entre 7 y 14 días y los que no recibieron IBP.

  13. Purification of carbon nanotubes grown by thermal CVD

    Science.gov (United States)

    Porro, S.; Musso, S.; Vinante, M.; Vanzetti, L.; Anderle, M.; Trotta, F.; Tagliaferro, A.

    2007-03-01

    We show the results of a set of purifications on carbon nanotubes (CNT) by acid and basic treatments. CNTs were obtained by thermal decomposition of camphor at 850 °C in a CVD growth system, by means of a growth process catalyzed by iron clusters originating from the addition of ferrocene in the precursors mixture. The purification procedures involved HNO 3, H 2SO 4, HSO 3Cl and NaOH for different process temperatures. As-grown CNTs showed a consistent presence of metal catalyst (about 6 wt%), evidenced by TGA. The purification treatments led to a certain amount of opening of the CNT tips, with a consequent loss of metal catalyst encapsulated in tips. This is also confirmed by BET analysis, which showed an increase of the surface area density of CNT after the purification. FT-IR and XPS revealed the presence of carboxylic groups on the CNT surface chemically modified by the harsh environment of the purification process. Among the various treatments that have been tested, the 1:3 solution of nitric and sulphuric acid was the most effective in modifying the CNT surface and inducing the formation of functional groups.

  14. Rapid purification of recombinant histones.

    Directory of Open Access Journals (Sweden)

    Henrike Klinker

    Full Text Available The development of methods to assemble nucleosomes from recombinant histones decades ago has transformed chromatin research. Nevertheless, nucleosome reconstitution remains time consuming to this day, not least because the four individual histones must be purified first. Here, we present a streamlined purification protocol of recombinant histones from bacteria. We termed this method "rapid histone purification" (RHP as it circumvents isolation of inclusion bodies and thereby cuts out the most time-consuming step of traditional purification protocols. Instead of inclusion body isolation, whole cell extracts are prepared under strongly denaturing conditions that directly solubilize inclusion bodies. By ion exchange chromatography, the histones are purified from the extracts. The protocol has been successfully applied to all four canonical Drosophila and human histones. RHP histones and histones that were purified from isolated inclusion bodies had similar purities. The different purification strategies also did not impact the quality of octamers reconstituted from these histones. We expect that the RHP protocol can be readily applied to the purification of canonical histones from other species as well as the numerous histone variants.

  15. Purification of Nanoparticles by Size and Shape

    Science.gov (United States)

    Robertson, James D.; Rizzello, Loris; Avila-Olias, Milagros; Gaitzsch, Jens; Contini, Claudia; Magoń, Monika S.; Renshaw, Stephen A.; Battaglia, Giuseppe

    2016-06-01

    Producing monodisperse nanoparticles is essential to ensure consistency in biological experiments and to enable a smooth translation into the clinic. Purification of samples into discrete sizes and shapes may not only improve sample quality, but also provide us with the tools to understand which physical properties of nanoparticles are beneficial for a drug delivery vector. In this study, using polymersomes as a model system, we explore four techniques for purifying pre-formed nanoparticles into discrete fractions based on their size, shape or density. We show that these techniques can successfully separate polymersomes into monodisperse fractions.

  16. The Status of KamLAND After Purification

    Science.gov (United States)

    Grant, Christopher

    2010-02-01

    KamLAND is a 1-kton liquid scintillation detector located in the Kamioka underground laboratory, in Japan. KamLAND has provided a precision measurement of δm^221 using reactor anti-neutrinos, and yielded first observational evidence of geologically produced anti-neutrinos. Since April of 2007, the collaboration has been working on the purification of the detector with the goal of observing 862 keV, ^7Be solar neutrinos. Two purification campaigns have concluded, with a total of 5.4 ktons of scintillator circulated through a distillation and nitrogen purge system. The results of purification and the overall background reduction factors will be presented, along with an update of the ^7Be solar neutrino analysis. )

  17. RELIGION AND PURIFICATION OF SOUL

    Directory of Open Access Journals (Sweden)

    Azam Khodashenas Pelko

    2010-11-01

    Full Text Available The Jainism emphasizes three major teachings about the purification of the soul (jiva, Ahimsa, Aparigrapha and anekantwad. Jainism, The focus of this religion has been purification of the soul by means of right conduct, right faith and right knowledge. The ultimate goal of Hinduism is Moksha or liberation (total freedom. In Hinduism, purification of the soul is a goal that one must work to attain. The Buddhism is the science of pursuing the aim of making the human mind perfect, and of purifying the human soul. The knowledge of purifying of the soul and softening of the hearts is as essential for human. They having the correct motivations means purifying our souls from hypocrisy, caprice, and heedlessness. The primary goal of Taoism may be described as the mystical intuition of the Tao, which is the way, the undivided unity, and the ultimate Reality. According to the Christianity access to truth cannot be conceived without purity of the soul

  18. Hydrogen purification by periodic adsorption

    Energy Technology Data Exchange (ETDEWEB)

    Barg, Christian; Secchi, Argimiro R.; Trierweiler, Jorge O. [Rio Grande do Sul Univ., Porto Alegre, RS (Brazil). Dept. de Engenharia Quimica]. E-mail: cbarg@enq.ufrgs.br; arge@enq.ufrgs.br; jorge@enq.ufrgs.br

    2000-07-01

    The periodic adsorption processes have been widely used for industrial applications, mainly because it spends less energy than the usual gas separation processes, like the cryogenic distillation. The largest commercial application of periodic adsorption processes is the pressure swing adsorption (PSA) applied to hydrogen purification. Although its wide use in the chemical and petrochemical industry, there are no reports in the open literature about complete modeling studies of a complex commercial unit, with multiple adsorbents and multiple beds and several feed components. This study has as objective the modeling, optimization and dynamical analysis of an industrial PSA unit for hydrogen purification. (author)

  19. Response Surface Methodology Modelling of an Aqueous Two-Phase System for Purification of Protease from Penicillium candidum (PCA 1/TT031 under Solid State Fermentation and Its Biochemical Characterization

    Directory of Open Access Journals (Sweden)

    Amaal M. Alhelli

    2016-11-01

    Full Text Available Penicillium candidum (PCA 1/TT031 synthesizes different types of extracellular proteases. The objective of this study is to optimize polyethylene glycol (PEG/citrate based on an aqueous two-phase system (ATPS and Response Surface Methodology (RSM to purify protease from Penicillium candidum (PCA 1/TT031. The effects of different PEG molecular weights (1500–10,000 g/mol, PEG concentration (9%–20%, concentrations of NaCl (0%–10% and the citrate buffer (8%–16% on protease were also studied. The best protease purification could be achieved under the conditions of 9.0% (w/w PEG 8000, 5.2% NaCl, and 15.9% sodium citrate concentration, which resulted in a one-sided protease partitioning for the bottom phase with a partition coefficient of 0.2, a 6.8-fold protease purification factor, and a yield of 93%. The response surface models displayed a significant (p ≤ 0.05 response which was fit for the variables that were studied as well as a high coefficient of determination (R2. Similarly, the predicted and observed values displayed no significant (p > 0.05 differences. In addition, our enzyme characterization study revealed that Penicillium candidum (PCA 1/TT031 produced a slight neutral protease with a molecular weight between 100 and 140 kDa. The optimal activity of the purified enzyme occurred at a pH of 6.0 and at a temperature of 50 °C. The stability between different pH and temperature ranges along with the effect of chemical metal ions and inhibitors were also studied. Our results reveal that the purified enzyme could be used in the dairy industry such as in accelerated cheese ripening.

  20. Response Surface Methodology Modelling of an Aqueous Two-Phase System for Purification of Protease from Penicillium candidum (PCA 1/TT031) under Solid State Fermentation and Its Biochemical Characterization

    Science.gov (United States)

    Alhelli, Amaal M.; Abdul Manap, Mohd Yazid; Mohammed, Abdulkarim Sabo; Mirhosseini, Hamed; Suliman, Eilaf; Shad, Zahra; Mohammed, Nameer Khairulla; Meor Hussin, Anis Shobirin

    2016-01-01

    Penicillium candidum (PCA 1/TT031) synthesizes different types of extracellular proteases. The objective of this study is to optimize polyethylene glycol (PEG)/citrate based on an aqueous two-phase system (ATPS) and Response Surface Methodology (RSM) to purify protease from Penicillium candidum (PCA 1/TT031). The effects of different PEG molecular weights (1500–10,000 g/mol), PEG concentration (9%–20%), concentrations of NaCl (0%–10%) and the citrate buffer (8%–16%) on protease were also studied. The best protease purification could be achieved under the conditions of 9.0% (w/w) PEG 8000, 5.2% NaCl, and 15.9% sodium citrate concentration, which resulted in a one-sided protease partitioning for the bottom phase with a partition coefficient of 0.2, a 6.8-fold protease purification factor, and a yield of 93%. The response surface models displayed a significant (p ≤ 0.05) response which was fit for the variables that were studied as well as a high coefficient of determination (R2). Similarly, the predicted and observed values displayed no significant (p > 0.05) differences. In addition, our enzyme characterization study revealed that Penicillium candidum (PCA 1/TT031) produced a slight neutral protease with a molecular weight between 100 and 140 kDa. The optimal activity of the purified enzyme occurred at a pH of 6.0 and at a temperature of 50 °C. The stability between different pH and temperature ranges along with the effect of chemical metal ions and inhibitors were also studied. Our results reveal that the purified enzyme could be used in the dairy industry such as in accelerated cheese ripening. PMID:27845736

  1. Electrostatic Precipitator Technique in the Purification Process of Central Air-conditioning Systems%静电除尘技术在中央空调系统净化改造中的应用

    Institute of Scientific and Technical Information of China (English)

    彭继

    2013-01-01

      结合中央空调净化系统的发展和静电除尘器的优点,通过分析目前中央空调系统中存在的问题和现有空调净化方式的不足,说明静电除尘净化方式可以解决目前空调系统中造成的室内空气污染及洁净室空调运行成本高等问题,能够使人们享受到更加洁净健康舒适的室内环境,使真正意义上的洁净空调系统得到推广。%The problems existed in central air-conditioning systems are analyzed, as well as the disadvantages of the present air-condi-tioning systems purifying methods. It also expounded the current development status of central air-conditioning systems and the advantages of the electrostatic precipitators. The analysis proved that the electrostatic dust purification method can resolve the indoor air pollution problems and the high operation cost of the air-conditioning systems in clean room effectively. The method will supply a better indoor environment which is more healthy and comfortable. This work will promote the popularization of the real clean air-conditioning systems.

  2. Generation and Purification of Atomic Entangled States

    Institute of Scientific and Technical Information of China (English)

    YANG Ming; SONG Wei; LI Yingqun; SHI Shouhua; CAO Zhuoliang

    2004-01-01

    @@ Entangled state plays a more and more important role in quantum information, so the generation of entangled state is of scientific value and practical significance.Although the experimental realization of entangled pairs of atoms and polarized photons have been reported recently, the current preparation schemes cannot meet the need of the practical application of entangled state in Quantum Communication and Quantum Computation.At the same time, resulting from the coupling between the quantum systems and its environment, decoherence of the quantum systems is unavoidable, which sets a vital obstacle on the way of the application of entanglement.There exist some entanglement generation and purification schemes, but the range of its application is relative small.So we proposed a more efficient scheme for entanglement generation and purification.The scheme is mainly based on the combination of linear optics and Cavity QED technique.The entanglement generation scheme can entangle two atoms by using MZI plus an optical cavity.Pure maximally entangled atomic states can be generated from product states or mixed states.Using a MZI, we can extract not only two-atom near-maximally entangled states but also four-atom maximally entangled states from less entangled pure or mixed states.

  3. Purification and properties of dialkylfluorophosphatase

    NARCIS (Netherlands)

    Cohen, J.A.; Warringa, M.G.P.J.

    1957-01-01

    1. 1. Zone electrophoresis on starch columns of purified preparations of fluorophosphatase resulted in a further purification. The preparations thus obtained differed in various respects from the cruder ones so far described. 2. 2. In the course of this electrophoresis fractions were obtained, which

  4. Quantum entanglement purification in cavities

    CERN Document Server

    Romero, J L; Saavedra, C; Retamal, J C

    2002-01-01

    A physical implementation of an entanglement purification protocol is studied using a cavity quantum electrodynamic based proposal, where, the quantum information is stored in quantum field sates inside cavities. Also a procedure is given for quantifying the degree of entanglement between quantum fields. (Author)

  5. [Immobilized microorganisms and water purification].

    Science.gov (United States)

    Mogilevich, N F

    1995-01-01

    Advantages and disadvantages of cells of aerobic microorganisms immobilized by the type of adhesion and incorporation into the gel beads, the amount of retained biomass, limitations of diffusion of oxygen and nutrients, viability, morphology, biochemical properties are described. Immobilized biocatalysts are discussed in the aspect of their use in purification of sewage waters.

  6. Biobased monoliths for adenovirus purification.

    Science.gov (United States)

    Fernandes, Cláudia S M; Gonçalves, Bianca; Sousa, Margarida; Martins, Duarte L; Barroso, Telma; Pina, Ana Sofia; Peixoto, Cristina; Aguiar-Ricardo, Ana; Roque, A Cecília A

    2015-04-01

    Adenoviruses are important platforms for vaccine development and vectors for gene therapy, increasing the demand for high titers of purified viral preparations. Monoliths are macroporous supports regarded as ideal for the purification of macromolecular complexes, including viral particles. Although common monoliths are based on synthetic polymers as methacrylates, we explored the potential of biopolymers processed by clean technologies to produce monoliths for adenovirus purification. Such an approach enables the development of disposable and biodegradable matrices for bioprocessing. A total of 20 monoliths were produced from different biopolymers (chitosan, agarose, and dextran), employing two distinct temperatures during the freezing process (-20 °C and -80 °C). The morphological and physical properties of the structures were thoroughly characterized. The monoliths presenting higher robustness and permeability rates were further analyzed for the nonspecific binding of Adenovirus serotype 5 (Ad5) preparations. The matrices presenting lower nonspecific Ad5 binding were further functionalized with quaternary amine anion-exchange ligand glycidyltrimethylammonium chloride hydrochloride by two distinct methods, and their performance toward Ad5 purification was assessed. The monolith composed of chitosan and poly(vinyl) alcohol (50:50) prepared at -80 °C allowed 100% recovery of Ad5 particles bound to the support. This is the first report of the successful purification of adenovirus using monoliths obtained from biopolymers processed by clean technologies.

  7. Effect of IDA and TREN chelating agents and buffer systems on the purification of human IgG with immobilized nickel affinity membranes.

    Science.gov (United States)

    Ribeiro, Mariana Borsoi; Vijayalakshmi, Mookambesvaran; Todorova-Balvay, Daniele; Bueno, Sonia Maria Alves

    2008-01-01

    The purification of IgG from human plasma was studied by comparing two affinity membranes complexed with Ni(II), prepared by coupling iminodiacetic acid (IDA) and Tris(2-aminoethyl)amine (TREN) to poly(ethylenevinyl alcohol), PEVA, hollow fiber membranes. The Ni(II)-TREN-PEVA hollow fiber membrane had lower capacity for human IgG than the complex Ni(II)-IDA-PEVA, but with similar selectivity. The IgG in peak fractions eluted from the Ni(II)-IDA-PEVA with a stepwise concentration gradient of Tris-HCl pH 7.0 (100-700 mM) reached a purity of 98% (based on IgG, IgM, IgA, albumin, and transferrin nephelometric analysis). Adsorption IgG data at different temperatures (4-37 degrees C) were analyzed using Langmuir model resulting in a calculated maximum capacity at 25 degrees C of 204.6 mg of IgG/g of dry membrane. Decrease in Kd with increasing temperature (1.7x10(-5) to 5.3x10(-6) M) indicated an increase in affinity with increased temperature. The positive value of enthalpy change (26.2 kJ/mol) indicated that the adsorption of IgG in affinity membrane is endothermic. Therefore, lower temperature induces adsorption as verified experimentally.

  8. Proton pump inhibitors in gastroesophageal reflux disease: a custom-tailored therapeutic regimen Diseñando un traje a la medida: los inhibidores de la bomba de protones en la enfermedad por reflujo gastroesofágico

    Directory of Open Access Journals (Sweden)

    Sergio Sobrino-Cossío

    2012-07-01

    Full Text Available The Montreal Definition and Classification divides Gastroesophageal Reflux Disease (GERD into esophageal symptomatic syndromes (and with mucosal damage and extraesophageal syndromes (with acid established association and proposed association. In typical GERD symptoms, an 8-week treatment with PPIs is satisfactory in most cases (> 90%. Response rates to PPIs in GERD are highly variable, as they also rely on an appropriate clinical diagnosis of the disease; endoscopy differentiates the macroscopic GERD phenotype. The non-erosive variety (50-70% prevalence has a different symptomatic response rate, as gastric acid is not the sole etiology of symptoms. The possible explanations of treatment failure include treatment adherence, PPI metabolism alterations and characteristics, and inadequate diagnosis. Refractory symptoms are related to gastric content neutralization by the chronic use of PPIs. Extraesophageal manifestations are associated with other pathophysiological mechanisms where an autonomic nervous system disturbance gives rise to symptoms. In these clinical entities, the relationship between symptoms and acid needs to be established in order to determine the use of PPIs, or consider other drugs. In other words, so as to "custom-tailor the best-fitting therapy" we need to answer the questions for whom, for what, how and for how long. Finally, PPI safety and tolerability are factors to be considered in elderly patients requiring chronic PPI use, who usually have chronic concomitant illnesses.La Clasificación de Montreal divide la enfermedad por reflujo gastro-esofágico (ERGE en síndromes esofágicos sintomáticos y con daño a la mucosa y en síndromes extraesofágicos con asociación establecida al ácido y asociación propuesta. En síntomas ERGE típicos el tratamiento con inhibidores de la bomba de protones (IBP es satisfactorio (> 90% a 8 semanas en la mayoría de los casos. Las tasas de respuesta a los IBP en la ERGE son muy variables

  9. High-speed countercurrent chromatography for purification of single-walled carbon nanotubes

    Institute of Scientific and Technical Information of China (English)

    Ying Cai; Zhi Hong Yan; Ying Chun Lv; Min Zi; Li Ming Yuan

    2008-01-01

    A new chromatographic purification of single-walled carbon nanotubes using high-speed countercurrent chromatography is reported.The purification was accomplished on the basis of experiment that dispersed the single-walled carbon nanotubes with sodium dodecyl sulfate,and the result mixture was separated using the two phase system composed of n-butanol/water=1/1 (v/v).The sizes of SWNTs separated were observed by scanning electron microscopy.The results demonstrated that the high-speed countercurrent chromatography possessed a good efficency for purification of single-walled carbon nanotubes.

  10. Use of Escherichia coli for the production and purification of membrane proteins.

    Science.gov (United States)

    Postis, Vincent G L; Rawlings, Andrea E; Lesiuk, Amelia; Baldwin, Stephen A

    2013-01-01

    Individual types of ion channels and other membrane proteins are typically expressed only at low levels in their native membranes, rendering their isolation by conventional purification techniques difficult. The heterologous over-expression of such proteins is therefore usually a prerequisite for their purification in amounts suitable for structural and for many functional investigations. The most straightforward expression host, suitable for prokaryote membrane proteins and some proteins from eukaryotes, is the bacterium Escherichia coli. Here we describe the use of this expression system for production of functionally active polytopic membrane proteins and methods for their purification by affinity chromatography in amounts up to tens of milligrams.

  11. [Assessment of schemes for sewage purification from petroleum products, by using various flotation methods].

    Science.gov (United States)

    Zabuga, G A; Filippova, T M; Sivkov, A A

    2010-01-01

    Petroleum products are the most common pollutants in petroleum refinery wastewater and are freed from the latter by flotation that is one of the most frequently applied physicochemical methods. The existing petroleum refinery OAO "Angara Petroleum Company" scheme for sewage purification from petroleum products, by using pressure flotation and proposed as a competitive purification scheme by applying electrical and impeller flotations underwent a comparative ecologoeconomic analysis. The use of electrical flotation instead of pressure flotation and that of an impeller flotation-electrical flotation system instead of a mechanical purification-pressure flotation one can considerably lower the concentration of petroleum products at the wastewater outlet into the Angara river.

  12. Partial purification of glucose-6-phosphate dehydrogenase by aqueous two-phase poly(ethyleneglycol/phosphate systems Purificação parcial de glucose-6-fosfato desidrogenase por sistemas de duas fases aquosas poli (etilenoglicol/fosfato

    Directory of Open Access Journals (Sweden)

    Marcela Zanella Ribeiro

    2007-03-01

    Full Text Available Glucose-6-phosphate dehydrogenase (G6PDH is an important enzyme used in biochemical and medical studies and in several analytical methods that have industrial and commercial application. This work evaluated the extraction of G6PDH in aqueous two-phase system (ATPS of poly(ethyleneglycol (PEG/phosphate buffer, using as enzyme source a medium prepared through commercial baker's yeast disruption. Firstly, the effects of PEG molar mass on the enzyme partition and of homogenization and rest on the system equilibrium were investigated. Afterwards, several ATPS were prepared using statistical analysis (2² factorial design. The results, including kinetic and thermodynamic parameters for the G6PDH activity, showed partial purification of this enzyme in ATPS composed of 17.5% (w/w PEG400 and 15.0% (w/w phosphate. A high enzymatic recovery value (97.7%, a high partition coefficient (351, and an acceptable purification factor (2.28 times higher than in cell homogenate were attained from the top phase. So, it was possible to attain an effective enzyme pre-purification by separating some contaminants with a simple method such as liquid-liquid extraction in aqueous two-phase systems (ATPS.Glicose-6-fosfato desidrogenase (G6PDH é uma importante enzima usada em estudos bioquímicos e médicos, bem como em diversos métodos analíticos com aplicação comercial e industrial. Neste trabalho foi avaliado a extração da G6PDH em sistemas de duas fases aquosas (ATPS constituídos por poli(etilenoglicol (PEG/tampão fosfato, usando como fonte de enzima um meio preparado por rompimento de leveduras de panificação comercial. Inicialmente foram investigados os efeitos da massa molar do PEG na partição da enzima e da homogeneização e repouso no equilíbrio do sistema. Na sequência, diversos ATPS foram preparados usando análise estatística (planejamento fatorial 2². Os resultados, incluindo parâmetros cinéticos e termodinâmicos para a atividade da G6PDH

  13. Membrane adsorbers as purification tools for monoclonal antibody purification.

    Science.gov (United States)

    Boi, Cristiana

    2007-03-15

    Downstream purification processes for monoclonal antibody production typically involve multiple steps; some of them are conventionally performed by bead-based column chromatography. Affinity chromatography with Protein A is the most selective method for protein purification and is conventionally used for the initial capturing step to facilitate rapid volume reduction as well as separation of the antibody. However, conventional affinity chromatography has some limitations that are inherent with the method, it exhibits slow intraparticle diffusion and high pressure drop within the column. Membrane-based separation processes can be used in order to overcome these mass transfer limitations. The ligand is immobilized in the membrane pores and the convective flow brings the solute molecules very close to the ligand and hence minimizes the diffusional limitations associated with the beads. Nonetheless, the adoption of this technology has been slow because membrane chromatography has been limited by a lower binding capacity than that of conventional columns, even though the high flux advantages provided by membrane adsorbers would lead to higher productivity. This review considers the use of membrane adsorbers as an alternative technology for capture and polishing steps for the purification of monoclonal antibodies. Promising industrial applications as well as new trends in research will be addressed.

  14. 从分子筛纯化系统的设计及操作谈空分设备节能%Approach to energy-saving for air separation plant from the design and operation of molecular sieve purification system

    Institute of Scientific and Technical Information of China (English)

    张振友

    2012-01-01

    The energy-saving for molecular sieve purification system of air separation plant is analyzed from the process design of molecular sieve purification system, the design and selection of equipment, the engineering design and its operation and maintenance, and%从分子筛纯化系统的流程设计、设备的设计与选型、工程设计及其操作、维护等方面,分析空分设备分子筛纯化系统的节能问题,提出了对节能措施的一些思考和建议。

  15. Liquid metal flow control by DC electromagnetic pumps; Controle de vazao de metais liquidos por bombas eletromagneticas de corrente continua

    Energy Technology Data Exchange (ETDEWEB)

    Borges, Eduardo Madeira; Braz Filho, Francisco Antonio; Guimaraes, Lamartine Nogueira Frutuoso [Instituto de Estudos Avancados (IEAv), Sao Jose dos Campos, SP (Brazil)], e-mail: eduardo@ieav.cta.br, e-mail: fbraz@ieav.cta.br, e-mail: guimarae@ieav.cta.br

    2006-07-01

    The cooling system of high-density thermal power requires fluids of high thermal conductivity, such as liquid metals. Electromagnetic pumps can be used to liquid metal fluid flow control in cooling circuits. The operation of electromagnetic pumps used to flow control is based on Lorentz force. This force can be achieved by magnetic field and electric current interaction, controlled by external independent power supplies. This work presents the electromagnetic pump operational principles, the IEAv development scheme and the BEMC-1 simulation code. The theoretical results of BEMC-1 simulation are compared to electromagnetic pump operation experimental data, validating the BEMC-1 code. This code is used to evaluate the DC electromagnetic pump performance applied to Mercury flow control and others liquid metal such as Sodium, Lead and Bismuth, used in nuclear fast reactors. (author)

  16. Electrophoretic High Molecular Weight DNA Purification Enables Optical Mapping

    Science.gov (United States)

    Maydan, Jason; Thomas, Matthew; Tabanfar, Leyla; Mai, Laura; Poon, Hau-Ling; Pe, Joel; Hahn, Kristen; Goji, Noriko; Amoako, Kingsley; Marziali, Andre; Hanson, Dan

    2013-01-01

    Optical mapping generates an ordered restriction map from single, long DNA molecules. By overlapping restriction maps from multiple molecules, a physical map of entire chromosomes and genomes is constructed, greatly facilitating genome assembly in next generation sequencing projects, comparative genomics and strain typing. However, optical mapping relies on a method of preparing high quality DNA >250 kb in length, which can be challenging from some organisms and sample types. Here we demonstrate the ability of Boreal Genomics' Aurora instrument to provide pure, high molecular weight (HMW) DNA 250-1,100 kb in length, ideally suited for optical mapping. The Aurora performs electrophoretic DNA purification within an agarose gel in reusable cartridges, protecting long DNA molecules from shearing forces associated with liquid handling steps common to other purification methods. DNA can be purified directly from intact cells embedded and lysed within an agarose gel, preserving the highest molecular weight DNA possible while achieving exceptional levels of purity. The Aurora delivers DNA in a buffer solution, where DNA can be condensed and protected from shearing during recovery with a pipette. DNA is then returned to its regular coiled state by simple dilution prior to optical mapping. Here we present images showing HMW DNA purification taking place in the Aurora and subsequent images of single DNA molecules on OpGen's Argus® Optical Mapping System. Future work will focus on further optimizing Aurora HMW DNA purification to bias DNA recovery in favor of only the longest molecules in a sample, maximizing the benefits of optical mapping.

  17. Purification of gibberellin sub 53 -oxidase from spinach

    Energy Technology Data Exchange (ETDEWEB)

    Wilson, T.M.; Zeevaart, J.A.D. (Michigan State Univ., East Lansing (USA))

    1989-04-01

    Spinach is a long-day rosette plants, in which stem growth is mediated by gibberellins. It has been shown that two enzymatic steps, GA{sub 53}-oxidase and GA{sub 19}-oxidase, are controlled by light. To develop an understanding into this light regulation, purification of GA{sub 53}-oxidase has been undertaken. The original assay relied on the HPLC separation of the product and substrate, but was considered too slow for the development of a purification scheme. A TLC system was developed which in conjunction with improvements to the assay conditions was sensitive and gave rapid results. The partial purification of the GA{sub 53}-oxidase is achieved by a high speed centrifugation, 40-55% ammonium sulfate precipitation, an hydroxyapatite column, Sephadex G-100 column and an anion exchange FPLC column, Mono Q HR10/10, yielding 1000-fold purification and 15% recovery. Monoclonal antibodies to the protein will be raised and used to further characterize the enzyme.

  18. Advanced purification of petroleum refinery wastewater by catalytic vacuum distillation.

    Science.gov (United States)

    Yan, Long; Ma, Hongzhu; Wang, Bo; Mao, Wei; Chen, Yashao

    2010-06-15

    In our work, a new process, catalytic vacuum distillation (CVD) was utilized for purification of petroleum refinery wastewater that was characteristic of high chemical oxygen demand (COD) and salinity. Moreover, various common promoters, like FeCl(3), kaolin, H(2)SO(4) and NaOH were investigated to improve the purification efficiency of CVD. Here, the purification efficiency was estimated by COD testing, electrolytic conductivity, UV-vis spectrum, gas chromatography-mass spectrometry (GC-MS) and pH value. The results showed that NaOH promoted CVD displayed higher efficiency in purification of refinery wastewater than other systems, where the pellucid effluents with low salinity and high COD removal efficiency (99%) were obtained after treatment, and the corresponding pH values of effluents varied from 7 to 9. Furthermore, environment estimation was also tested and the results showed that the effluent had no influence on plant growth. Thus, based on satisfied removal efficiency of COD and salinity achieved simultaneously, NaOH promoted CVD process is an effective approach to purify petroleum refinery wastewater. Copyright 2010 Elsevier B.V. All rights reserved.

  19. Turnkey Helium Purification and Liquefaction Plant for DARWIN, Australia

    Science.gov (United States)

    Lindemann, U.; Boeck, S.; Blum, L.; Kurtcuoglu, K.

    2010-04-01

    The Linde Group, through its Australian subsidiary BOC Limited, has signed an agreement with Darwin LNG Pty Ltd for the supply of feed-gas to Linde's new helium refining and liquefaction facility in Darwin, Australia. Linde Kryotechnik AG, located in Switzerland, has carried out the engineering and fabrication of the equipment for the turn key helium plant. The raw feed gas flow of 20'730 Nm3/h contains up to of 3 mol% helium. The purification process of the feed gas consists of partial condensation of nitrogen in two stages, cryogenic adsorption and finally catalytic oxidation of hydrogen followed by a dryer system. Downstream of the purification the refined helium is liquefied using a modified Bryton process and stored in a 30'000 gal LHe tank. For further distribution and export of the liquid helium there are two stations available for filling of truck trailers and containers. The liquid nitrogen, required for refrigeration capacity to the nitrogen removal stages in the purification process as well as for the pre-cooling of the pure helium in the liquefaction process, is generated on site during the feed gas purification process. The optimized process provides low power consumption, maximum helium recovery and a minimum helium loss.

  20. Purification of Tetrahymena cytoskeletal proteins.

    Science.gov (United States)

    Honts, Jerry E

    2012-01-01

    Like all eukaryotic cells, Tetrahymena thermophila contains a rich array of cytoskeletal proteins, some familiar and some novel. A detailed analysis of the structure, function, and interactions of these proteins requires procedures for purifying the individual protein components. Procedures for the purification of actin and tubulin from Tetrahymena are reviewed, followed by a description of a procedure that yields proteins from the epiplasmic layer and associated structures, including the tetrins. Finally, the challenges and opportunities for future advances are assessed.

  1. Comparing Russian and Finnish standards of water purification

    OpenAIRE

    Maria, Pupkova

    2012-01-01

    The subject of this thesis is water purification. The first aim of this thesis is to consider different ways of water purification. The second aim is to compare Finnish and Russian standards of water purification. The third one is to show water purification methods on the pattern of Mikkeli water purification plan. Water purification methods of water intended for human consumption will be described.Combined tables will be done according to the quality requirement of drinking water of both,...

  2. Purification technology of molten aluminum

    Institute of Scientific and Technical Information of China (English)

    孙宝德; 丁文江; 疏达; 周尧和

    2004-01-01

    Various purification methods were explored to eliminate the dissolved hydrogen and nonmetallic inclusions from molten aluminum alloys. A novel rotating impeller head with self-oscillation nozzles or an electromagnetic valve in the gas circuit was used to produce pulse gas currents for the rotary impeller degassing method. Water simulation results show that the size of gas bubbles can be decreased by 10%-20% as compared with the constant gas current mode. By coating ceramic filters or particles with active flux or enamels, composite filters were used to filter the scrap A356 alloy and pure aluminum. Experimental results demonstrate that better filtration efficiency and operation performance can be obtained. Based on numerical calculations, the separation efficiency of inclusions by high frequency magnetic field can be significantly improved by using a hollow cylinder-like separator or utilizing the effects of secondary flow of the melt in a square separator. A multi-stage and multi-media purification platform based on these methods was designed and applied in on-line processing of molten aluminum alloys. Mechanical properties of the processed scrap A356 alloy are greatly improved by the composite purification.

  3. Technological assumptions for biogas purification.

    Science.gov (United States)

    Makareviciene, Violeta; Sendzikiene, Egle

    2015-01-01

    Biogas can be used in the engines of transport vehicles and blended into natural gas networks, but it also requires the removal of carbon dioxide, hydrogen sulphide, and moisture. Biogas purification process flow diagrams have been developed for a process enabling the use of a dolomite suspension, as well as for solutions obtained by the filtration of the suspension, to obtain biogas free of hydrogen sulphide and with a carbon dioxide content that does not exceed 2%. The cost of biogas purification was evaluated on the basis of data on biogas production capacity and biogas production cost obtained from local water treatment facilities. It has been found that, with the use of dolomite suspension, the cost of biogas purification is approximately six times lower than that in the case of using a chemical sorbent such as monoethanolamine. The results showed travelling costs using biogas purified by dolomite suspension are nearly 1.5 time lower than travelling costs using gasoline and slightly lower than travelling costs using mineral diesel fuel.

  4. Nanostructured Catalytic Reactors for Air Purification Project

    Data.gov (United States)

    National Aeronautics and Space Administration — This SBIR Phase I project proposes the development of lightweight compact nanostructured catalytic reactors for air purification from toxic gaseous organic...

  5. Nanostructured Catalytic Reactors for Air Purification Project

    Data.gov (United States)

    National Aeronautics and Space Administration — This SBIR Phase II project proposes the development of lightweight compact nanostructured catalytic reactors for air purification from toxic gaseous organic...

  6. Rapid Purification and Characterization of Mutant Origin Recognition Complexes in Saccharomyces cerevisiae

    OpenAIRE

    Hironori eKawakami; Eiji eOhashi; Toshiki eTsurimoto; Tsutomu eKatayama

    2016-01-01

    Purification of the origin recognition complex (ORC) from wild-type budding yeast cells more than two decades ago opened up doors to analyze the initiation of eukaryotic chromosomal DNA replication biochemically. Although revised methods to purify ORC from overproducing cells were reported later, purification of mutant proteins using these systems still depends on time-consuming processes including genetic manipulation to construct and amplify mutant baculoviruses or yeast strains as well as ...

  7. Energy saving in the auxiliaries consumption for circulation water pumps optimizing the thermal regime; Ahorro en el consumo de auxiliares por bombas de agua de circulacion optimando el regimen termico

    Energy Technology Data Exchange (ETDEWEB)

    Orozco Martinez, Roni [Instituto de Investigaciones Electricas, Cuernavaca (Mexico)

    1998-12-31

    A methodology is proposed that should be followed in any thermal electric power plant to determine the load value at which a unit requires a second circulation water pump without affecting the thermal regime and avoiding an excessive auxiliaries consumption in partial loads. In applying this method the power plant would have an energy saving equivalent to the auxiliaries consumption during an hour, when the unit as operating at full load. [Espanol] Se propone una metodologia que debe seguirse en cualquier central termoelectrica para determinar el valor de la carga en la cual una unidad requiere de la segunda bomba de agua de circulacion sin afectar el regimen termico y evitadose un excesivo consumo de auxiliares en cargas parciales. Al aplicar este metodo la central tendria un ahorro de energia equivalente al consumo de auxiliares durante una hora cuando la unidad esta generando su maxima carga.

  8. Desenvolvimento de um projeto para construção de bomba-balão para contrapulsação aórtica Development and construction of a balloon-pump for aortic counterpulsation

    OpenAIRE

    Celso Luiz dos Reis; Paulo Roberto Barbosa Évora; José Carlos Franco Brasil; Paulo José de Freitas Ribeiro; Adonis Garcia Otaviano; Hércules Lisboa Bongiovani; Rúbio Bombonato; Marcus Antônio Ferez; Ricardo Nilsson Sgarbieri; Francisco Fernandes Moreira Neto; Almir Sales Pereira; Percival Gomes

    1989-01-01

    O presente trabalho tem por finalidade apresentar as etapas de um projeto para construção de uma bomba-balâo para contrapulsação aórtica. Desenvolveram-se três protótipos, sendo que os dois primeiros utilizavam ar comprimido e vácuo hospitalares para insuflação e deflação do cateter-balão. Esses dois protótipos apresentavam como diferenças fundamentais: o tipo de dispositivo que captava o sinal luminoso da onda R do ECG utilizado para a sincronização do bombeamento; o tipo de válvula solenóid...

  9. 165. El corazón como bomba de succión cardíaca a partir del análisis electrofisiológico mediante carto. implicaciones quirúrgicas

    OpenAIRE

    Trainini, J.C.; Herreros, J.; Bustamante, J.; Elencwajg, B.; García-Morán, E.; López Cabanillas, N.; Otero, E.; Valle, J.

    2012-01-01

    Según la hipótesis de Torrent-Guasp el corazón es una banda miocárdica sin apoyos fijos, generadora de reducción tridimensional y comportamiento de bomba de succión. Nuestro objetivo fue analizar la secuencia de activación y correlacionarlo con la teoría de Torrent-Guasp. Métodos: Se realizó estudio electrofisiológico con el programa Carto a 4 pacientes sin cardiopatía estructural de forma concomitante a ablación de fibrilación auricular (FA). Los mapas isocrónicos y de secuencia de activa...

  10. Diseño, construcción y puesta a punto de una instalación para la determinación de la cavitación en bombas centrífugas

    OpenAIRE

    Barcina García, Pedro

    2016-01-01

    El presente proyecto se basa en el diseño, construcción y puesta a punto de una instalación para el estudio de la cavitación en bombas centrífugas. Es una profundización, desde el punto de vista experimental, en los conocimientos aprendidos sobre el tema en las asignaturas de Ingeniería Fluidomecánica, Mecánica de Fluidos y Máquinas Hidráulicas. Además del experimento, se ha preparado la instalación y se ha redactado un guion de prácticas para que pueda ser usada con fines didácticos por los ...

  11. 165. El corazón como bomba de succión cardíaca a partir del análisis electrofisiológico mediante carto. implicaciones quirúrgicas

    Directory of Open Access Journals (Sweden)

    J.C. Trainini

    2012-04-01

    Conclusiones: La contracción sucesiva de los segmentos de la gran banda muscular genera un ciclo cardíaco continuo (motus perpetuus cordis. La fase diastólica de contracción isovolumétrica implica considerar al corazón una bomba de succión y desarrollar nuevas interpretaciones fisiopatológicas y clasificaciones de la insuficiencia cardíaca basadas en la succión. El valor del ápex en la cirugía de restauración, la contracción diastólica de la banda ascendente y la sincronización de la doble banda promueve el desarrollo de nuevos dispositivos y técnicas quirúrgicas.

  12. 水雾净化装置在火炬系统整改中的工艺研究%Study on the mist purification device used in flare gas system reform

    Institute of Scientific and Technical Information of China (English)

    高跃峰

    2012-01-01

    Flare gas have a lot of energy and useful component, recovery of flare gas is a ma- jor energy-saving measures. However, flare gas composition is not only complex and high dust, often blocked downstream piping and compressor flow, reducing the recovery efficiency, increased operating and maintenance costs. In this transformation of the system increased mist purification device, and to process the calculation of the flare gas tank which is on one of the main equipment.%石化装置火炬气中蕴藏着大量的能量和有用成分,回收火炬气是一项重大节能措施。然而火炬气成分复杂含尘量高,经常堵塞下游管路及压缩机流道,降低了回收效率,增加了运行及维护成本。本次系统改造在火炬气管路中增加了水雾净化系统,并就其中的主要设备火炬气处理气罐进行了工艺计算。

  13. 浅议丙酮肟在引进分厂锅炉给水系统中的应用%Application of acetone oxime in the boiler feed system in Injin Natural Gas Purification Plant

    Institute of Scientific and Technical Information of China (English)

    唐浠; 瞿杨; 蒋宇; 吴高亮; 秦婧

    2014-01-01

    Because of the reasons that hydrazine is flammable and explosive ,slowly reacts with oxygen ,toxic ,etc .,it is substituted by acetone oxime as deoxidant in Yinjin Branch of Chongqing Natural Gas Purification Plant General .Analysis results show that the acetone oxime has better deoxidization effect and lower toxicity than hydrazine ,and it does not affect the water vapor system ,so that it can protect the equipment and has inhibit effect .Further more ,acetone oxime can also reduce the production cost ,therefore ,it is more economical and practical .%重庆天然气净化总厂引进分厂原来在锅炉给水系统中一直使用联氨作为除氧剂,但由于联氨易燃易爆、除氧速度慢、具有毒性等原因,则采用丙酮肟代替联氨进行除氧。分析使用丙酮肟除氧后的数据,丙酮肟较联氨而言,除氧效果更好,毒性更低,不影响水汽系统,对设备有保护作用和缓蚀效果,并减少了生产成本,更具有经济效益和实用性。

  14. An Inducible Retroviral Expression System for Tandem Affinity Purification Mass-Spectrometry-Based Proteomics Identifies Mixed Lineage Kinase Domain-like Protein (MLKL) as an Heat Shock Protein 90 (HSP90) Client.

    Science.gov (United States)

    Bigenzahn, Johannes W; Fauster, Astrid; Rebsamen, Manuele; Kandasamy, Richard K; Scorzoni, Stefania; Vladimer, Gregory I; Müller, André C; Gstaiger, Matthias; Zuber, Johannes; Bennett, Keiryn L; Superti-Furga, Giulio

    2016-03-01

    Tandem affinity purification-mass spectrometry (TAP-MS) is a popular strategy for the identification of protein-protein interactions, characterization of protein complexes, and entire networks. Its employment in cellular settings best fitting the relevant physiology is limited by convenient expression vector systems. We developed an easy-to-handle, inducible, dually selectable retroviral expression vector allowing dose- and time-dependent control of bait proteins bearing the efficient streptavidin-hemagglutinin (SH)-tag at their N- or C termini. Concomitant expression of a reporter fluorophore allows to monitor bait-expressing cells by flow cytometry or microscopy and enables high-throughput phenotypic assays. We used the system to successfully characterize the interactome of the neuroblastoma RAS viral oncogene homolog (NRAS) Gly12Asp (G12D) mutant and exploited the advantage of reporter fluorophore expression by tracking cytokine-independent cell growth using flow cytometry. Moreover, we tested the feasibility of studying cytotoxicity-mediating proteins with the vector system on the cell death-inducing mixed lineage kinase domain-like protein (MLKL) Ser358Asp (S358D) mutant. Interaction proteomics analysis of MLKL Ser358Asp (S358D) identified heat shock protein 90 (HSP90) as a high-confidence interacting protein. Further phenotypic characterization established MLKL as a novel HSP90 client. In summary, this novel inducible expression system enables SH-tag-based interaction studies in the cell line proficient for the respective phenotypic or signaling context and constitutes a valuable tool for experimental approaches requiring inducible or traceable protein expression.

  15. Variação da razão entre fluxo sanguíneo e rotações da bomba centrífuga como ferramenta para monitorar o desempenho do oxigenador durante o suporte respiratório extracorpóreo

    Directory of Open Access Journals (Sweden)

    Marcelo Park

    2015-06-01

    Full Text Available RESUMO Objetivo: Analisar as correlações da taxa de fluxo sanguíneo e rotação da bomba com a pressão transmembrana e a transferência de CO2 e O2 durante o suporte respiratório extracorpóreo. Métodos: Cinco animais foram instrumentalizados e submetidos à oxigenação extracorpórea de membrana em um protocolo de cinco fases, as quais incluíam sepse abdominal e lesão pulmonar. Resultados: Este estudo demonstrou que as variações da taxa de fluxo sanguíneo e rotação da bomba dependem, de forma logarítmica positiva, do fluxo sanguíneo na membrana extracorpórea de oxigenação. As variações da taxa de fluxo sanguíneo e rotação da bomba têm associação negativa com a pressão transmembrana (R2 = 0,5 para o fluxo sanguíneo = 1.500mL/minuto e R2 = 0,4 para o fluxo sanguíneo = 3.500mL/minuto, ambos com p 6L/minuto, p = 0,006. A taxa de fluxo sanguíneo com a rotação da bomba não se associa às variações na transferência de O2 (R2 = 0,01 para o fluxo sanguíneo = 1.500mL/minuto, p = 0,19, e R2 = -0,01 ao fluxo sanguíneo = 3.500mL/minuto, p = 0,46. Conclusão: Neste modelo em animais, a variação da taxa de fluxo sanguíneo e rotação da bomba se associa negativamente com a pressão transmembrana e positivamente com a transferência de CO2. Conforme a situação clínica, uma diminuição na taxa do fluxo sanguíneo e rotação da bomba pode, na ausência de hipoxemia, indicar uma disfunção do pulmão artificial.

  16. Purification and characterization of the Oligosaccharyl transferase

    Energy Technology Data Exchange (ETDEWEB)

    Kapoor, T.M.

    1990-11-01

    Oligosaccharyl transferase was characterized to be a glycoprotein with at least one saccharide unit that had a D-manno or D- glucopyranose configuration with unmodified hydroxy groups at C-3, C-4 and C-6, using a Concanavalin A affinity column. This afforded a 100 fold increase in the transferase purity in the solubilized microsomal sample and also removed over 90% of the microsomal proteins (the cytosolic ones being removed before solubilization). The detergent, N,N-Dimethyldodecylamine N-oxide (LDAO) was used for solubilization and it yielded a system compatible with the assay and the purification steps. An efficient method for detergent extraction without dilution of sample or protein precipitation was also developed.

  17. Bomba dlja Pitera / German Petelin

    Index Scriptorium Estoniae

    Petelin, German

    2004-01-01

    Venemaa aatomiteadlased Valeri Volkov ja Igor Ostretsov süüdistavad president Vladimir Putinit riigi tuumaalase julgeoleku ohtu seadmises, põhjuseks nn. "tšernobõli tüüpi" Leningradi aatomielektrijaama energobloki töö pikendamise õiguspärasus

  18. Bomba dlja Pitera / German Petelin

    Index Scriptorium Estoniae

    Petelin, German

    2004-01-01

    Venemaa aatomiteadlased Valeri Volkov ja Igor Ostretsov süüdistavad president Vladimir Putinit riigi tuumaalase julgeoleku ohtu seadmises, põhjuseks nn. "tšernobõli tüüpi" Leningradi aatomielektrijaama energobloki töö pikendamise õiguspärasus

  19. Treatment of hyperbilirubinemia with blood purification in China

    Institute of Scientific and Technical Information of China (English)

    Zhi-Jun Duan; Lei-Lei Li; Jia Ju; Zhi-Hong Gao; Gao-Hong He

    2006-01-01

    The incidence of hyperbilirubinemia is high clinically,which is difficult to cure by medication, surgery or interventional therapies. Non-bioartificial liver is the main alternative in the blood purification for hyperbilirubinemia, which includes plasma exchange,hemoperfusion, hemodialysis, molecular adsorbent recycling system and so on. The research results and clinical experiences in China show that these methods are effective in lowering high levels of bilirubin with fewer side effects. The hyperbilirubinemias of different causes, with different complications or accompanying different diseases can be treated by different methods.Bioartificial liver, hybrid artificial liver support system and adsorbent membrane material have also been studied and their development in reducing hyperbilirubinemias has been achieved. This article gives a brief overview on the actuality and research improvement in blood purification for hyperbilirubinemia in China.

  20. Substrate improvement and wastewater purification efficiency of subsurface infiltration system%地下渗滤系统填充基质改良及污水净化效果研究

    Institute of Scientific and Technical Information of China (English)

    潘晶; 孙铁珩; 李海波

    2011-01-01

    通过模拟土柱实验,利用炉渣、蛭石、水淬渣、砂子等廉价易得的材料来改善草甸棕壤的渗透性能,并对构建的4组地下渗滤系统在冬季和夏季的污水处理效果进行了研究.结果表明,加入改良材料后,改良渗滤基质的渗透性比草甸棕壤均有了显著提高,4种改良材料的最适添加质量比为5%;炉渣、蛭石、水淬渣、砂子、草甸棕壤的TP吸附行为均符合Langmuir模型,拟合的相关系数都在0.89以上,TP饱和吸附量分别为0.714、0.625、1.000、0.060、0.357 mg/g;冬季和夏季,4组地下渗滤系统对TP的去除效果相差不大,出水TP始终小于0.4 mg/L;4组地下渗滤系统夏季对COD、TN、NH4+-N的去除效果略高于冬季,无论冬季还是夏季,出水COD均低于43 mg/L,TN去除率均低于70%,出水的NH4+-N、TN浓度均达到(GB18918-2002)的一级标准.%The soil column experiments with meadow palm soil as substrate were conducted to simulate the water purification in subsurface infiltration system. Four cheap and available materials (boiler slag, vermiculite, watergranulated slag of blast-furnace and sand) were mixed in the substrate separately to improve the penetrability of infiltration system,the performance of 4 improved systems for water purification were detected. Results showed that the penetrability of meadow palm soil was greatly improved after mixing these materials, and the optimum mixing ratio was 5 %. The phosphorus adsorption behavior in boiler slag, vermiculite, water-granulated slag, sand and meadow palm soil could be well simulated by Langmuir equation with the correlation coefficient all above 0.89,the maximum phosphorus absorption capacities were 0. 714,0. 625,1. 000,0. 060,0. 357 mg/g, respectively. For the 4 improved infiltration system,TP removal in summer and in winter presented no significant difference,the effluent TP kept lower than 0. 4 mg/L; the removal efficiency of COD,TN and NH4+-N in summer was little higher

  1. Biogas Purification up to Final Product

    Directory of Open Access Journals (Sweden)

    Yu. Losiuk

    2012-01-01

    Full Text Available The paper considers main technological methods for biogas purification from impurities that permit to increase energy value of the product and decrease its corrosion activity.  While evaluating economic efficiency due to introduction of the corresponding purification technology, in addition, it is necessary to take into account an ecological factor.

  2. Boron carbide morphology changing under purification

    Science.gov (United States)

    Rahmatullin, I. A.; Sivkov, A. A.

    2015-10-01

    Boron carbide synthesized by using coaxial magnetoplasma accelerator with graphite electrodes was purified by two different ways. XRD-investigations showed content changing and respectively powder purification. Moreover TEM-investigations demonstrated morphology changing of product under purification that was discussed in the work.

  3. HOUSEHOLD PURIFICATION OF FLUORIDE CONTAMINATED MAGADI (TRONA)

    DEFF Research Database (Denmark)

    1997-01-01

    Purification of fluoride contaminated magadi is studied using bone char sorption and calcium precipitation. The bone char treatment is found to be workable both in columns and in batches where the magadi is dissolved in water prior to treatment. The concentrations in the solutions were 89 g magadi...... treatment method. A procedure for purification of fluoride contaminated magadi at household level is described....

  4. Partial Purification and Characterization of Extracellular Protease ...

    African Journals Online (AJOL)

    USER

    Purification of the enzyme by gel filtration chromatography on Sephadex G75 following ammonium sulphate precipitation gave 2.26 fold increase in purification with specific activity of 46.13 .... minutes in a water bath and were allowed to cool.

  5. PURIFICATION OF TRANSFORMER OIL in PT. PJB UP PAITON

    OpenAIRE

    Gusti Wahdaniyah*, Purnomo Tri Prasetyo, Arif Setiabudi, Totok R. Biyanto

    2016-01-01

    The purpose of this paper is to describe the filtration or purification of transformer oil. One of the main equipment in coal-fired power generation unit is transformer. When the transformer fail to operate properly, the continuity of distribution system become interrupted. As a part of transformer, transformer oil contribute the failure of transformer due to the aging. To solve this problem, several methods is applied starting from dehydration process, degasification process, oxidation remov...

  6. Ecological aspects of the extreme purification of water

    Science.gov (United States)

    Shaposhnik, Vladimir A.; Mazo, A. A.; Frölich, P.

    1991-11-01

    The influence on the eco-system of the products of the large-scale technology for the preparation of ultra-pure water required for the electronic and radiotechnical industries is examined. The distillation, ion-exchange, and membrane methods are subjected to a comparative analysis. It is shown that the membrane method for the extreme purification of water is ecologically the most desirable. The methods for the elimination of nitrates from drinking water are examined. The bibliography includes 41 references.

  7. PURIFICATION OF TRANSFORMER OIL in PT. PJB UP PAITON

    OpenAIRE

    Gusti Wahdaniyah*, Purnomo Tri Prasetyo, Arif Setiabudi, Totok R. Biyanto

    2016-01-01

    The purpose of this paper is to describe the filtration or purification of transformer oil. One of the main equipment in coal-fired power generation unit is transformer. When the transformer fail to operate properly, the continuity of distribution system become interrupted. As a part of transformer, transformer oil contribute the failure of transformer due to the aging. To solve this problem, several methods is applied starting from dehydration process, degasification process, oxidation remov...

  8. 24 CFR 203.52 - Acceptance of individual residential water purification equipment.

    Science.gov (United States)

    2010-04-01

    ... water purification system is currently in operation on the property. If the system in operation employs... untreated water for flushing toilets may be constructed. (2) The system is sufficient to assure an... maintaining. I undertstand that the individual water supply is unsafe for consumption unless the system is...

  9. Cell-Type-Specific mRNA Purification by Translating Ribosome Affinity Purification (TRAP)

    Science.gov (United States)

    Heiman, Myriam; Kulicke, Ruth; Fenster, Robert J.; Greengard, Paul; Heintz, Nathaniel

    2014-01-01

    Cellular diversity and architectural complexity create barriers to understanding the function of the mammalian central nervous system (CNS) at a molecular level. To address this problem, we recently developed a methodology that provides the ability to profile the entire translated mRNA complement of any genetically defined cell population. This methodology, which we termed translating ribosome affinity purification, or TRAP, combines cell-type-specific transgene expression with affinity purification of translating ribosomes. TRAP can be used to study the cell-type-specific mRNA profiles of any genetically defined cell type, and has been successfully used to date in organisms ranging from D. melanogaster to mice and human cultured cells. Unlike other methodologies that rely upon micro-dissection, cell panning, or cell sorting, the TRAP methodology bypasses the need for tissue fixation or single-cell suspensions (and potential artifacts these treatments introduce), and reports on mRNAs in the entire cell body. This protocol provides a step-by-step guide to implementing the TRAP methodology, which takes two days to complete once all materials are in hand. PMID:24810037

  10. Optimization of conditions for the single step IMAC purification of miraculin from Synsepalum dulcificum.

    Science.gov (United States)

    He, Zuxing; Tan, Joo Shun; Lai, Oi Ming; Ariff, Arbakariya B

    2015-08-15

    In this study, the methods for extraction and purification of miraculin from Synsepalum dulcificum were investigated. For extraction, the effect of different extraction buffers (phosphate buffer saline, Tris-HCl and NaCl) on the extraction efficiency of total protein was evaluated. Immobilized metal ion affinity chromatography (IMAC) with nickel-NTA was used for the purification of the extracted protein, where the influence of binding buffer pH, crude extract pH and imidazole concentration in elution buffer upon the purification performance was explored. The total amount of protein extracted from miracle fruit was found to be 4 times higher using 0.5M NaCl as compared to Tris-HCl and phosphate buffer saline. On the other hand, the use of Tris-HCl as binding buffer gave higher purification performance than sodium phosphate and citrate-phosphate buffers in IMAC system. The optimum purification condition of miraculin using IMAC was achieved with crude extract at pH 7, Tris-HCl binding buffer at pH 7 and the use of 300 mM imidazole as elution buffer, which gave the overall yield of 80.3% and purity of 97.5%. IMAC with nickel-NTA was successfully used as a single step process for the purification of miraculin from crude extract of S. dulcificum.

  11. Water Purification by Shock Electrodialysis: Deionization, Filtration, Separation, and Disinfection

    CERN Document Server

    Deng, Daosheng; Braff, William A; Schlumpberger, Sven; Suss, Matthew E; Bazant, Martin Z

    2014-01-01

    The development of energy and infrastructure efficient water purification systems are among the most critical engineering challenges facing our society. Water purification is often a multi-step process involving filtration, desalination, and disinfection of a feedstream. Shock electrodialysis (shock ED) is a newly developed technique for water desalination, leveraging the formation of ion concentration polarization (ICP) zones and deionization shock waves in microscale pores near to an ion selective element. While shock ED has been demonstrated as an effective water desalination tool, we here present evidence of other simultaneous functionalities. We show that, unlike electrodialysis, shock ED can thoroughly filter micron-scale particles and aggregates of nanoparticles present in the feedwater. We also demonstrate that shock ED can enable disinfection of feedwaters, as approximately $99\\%$ of viable bacteria (here \\textit{E. coli}) in the inflow were killed or removed by our prototype. Shock ED also separates...

  12. Recombinant Dragline Silk-Like Proteins—Expression and Purification

    Science.gov (United States)

    Gaines, William A.; Marcotte, William R.

    2011-01-01

    Spider dragline silk is a proteinaceous fiber with impressive physical characteristics making it attractive for use in advanced materials. The fiber is composed of two proteins (spidroins MaSp1 and MaSp2), each of which contains a large central repeat array flanked by non-repetitive N- and C-terminal domains. The repeat arrays appear to be largely responsible for the tensile properties of the fiber, suggesting that the N- and C-terminal domains may be involved in self-assembly. We recently isolated the MaSp1 and MaSp2 N-terminal domains from Nephila clavipes and have incorporated these into mini-silk genes for expression in transgenic systems. Current efforts involve the development of expression vectors that will allow purification using a removable affinity tag for scalable protein purification. PMID:23914141

  13. Recombinant Dragline Silk-Like Proteins-Expression and Purification.

    Science.gov (United States)

    Gaines, William A; Marcotte, William R

    2011-03-01

    Spider dragline silk is a proteinaceous fiber with impressive physical characteristics making it attractive for use in advanced materials. The fiber is composed of two proteins (spidroins MaSp1 and MaSp2), each of which contains a large central repeat array flanked by non-repetitive N- and C-terminal domains. The repeat arrays appear to be largely responsible for the tensile properties of the fiber, suggesting that the N- and C-terminal domains may be involved in self-assembly. We recently isolated the MaSp1 and MaSp2 N-terminal domains from Nephila clavipes and have incorporated these into mini-silk genes for expression in transgenic systems. Current efforts involve the development of expression vectors that will allow purification using a removable affinity tag for scalable protein purification.

  14. Uranium hexafluoride purification; Purificacao de hexafluoreto de uranio

    Energy Technology Data Exchange (ETDEWEB)

    Araujo, Eneas F. de

    1986-07-01

    Uranium hexafluoride might contain a large amount of impurities after manufacturing or handling. Three usual methods of purification of uranium hexafluoride were presented: selective sorption, sublimation, and distillation. Since uranium hexafluoride usually is contaminated with hydrogen fluoride, a theoretical study of the phase equilibrium properties was performed for the binary system UF{sub 6}-HF. A large deviation from the ideal solution behaviour was observed. A purification unity based on a constant reflux batch distillation process was developed. A procedure was established in order to design the re boiler, condenser and packed columns for the UF{sub 6}-HF mixture separation. A bench scale facility for fractional distillation of uranium hexafluoride was described. Basic operations for that facility and results extracted from several batches were discussed. (author)

  15. Current topics of purification and constitutions of dialysis fluid.

    Science.gov (United States)

    Tomo, Tadashi

    2015-01-01

    Dialysis fluid is a fundamental component of hemodialysis treatment, and its roles include the correction of electrolyte levels, pH, and osmolality, as well as the removal of uremic solutes from the blood of patients with renal failure. In recent years, purification of dialysis fluid has become essential due to the use of high-flux membrane dialyzers. Therefore, rigorous standards have been established for the purification of dialysis fluid, which is becoming widely practiced in Japan. The effects of dialysis fluid purification include the prevention of micro-inflammation, preservation of residual renal function, improvement of nutritional status, and resolution of resistance to erythropoiesis-stimulating agents. When purifying the dialysis fluid used in the central dialysis fluid delivery system, validation of the system is also important. Dialysis fluid that does not contain acetate has become available, and there have been reports of decreased micro-inflammation, etc., with this innovation. In addition, dialysis fluid containing a higher concentration of bicarbonate than is conventionally employed has become available. Although correction of acidosis remains important, excess alkalosis may reportedly worsen the survival prognosis of hemodialysis patients. Sufficient attention should be paid to these issues.

  16. Purification for the XENONnT dark matter experiment

    Science.gov (United States)

    Brown, Ethan; Xenon Collaboration

    2017-01-01

    The XENON1T experiment uses 3.5 tons of liquid xenon in a cryogenic detector to search for dark matter. Its upgrade, XENONnT, will similarly house 7.5 tons of liquid xenon. Operation of these large detectors requires continual purification of the xenon in an external purifier, and the need for less than part per billion level oxygen in the xenon, coupled with the large quantity of xenon to be purified, places high demands on the rate of flow through this purification system. Building on the success of the XENON10 and XENON100 experiments, XENON1T circulates gaseous xenon through heated getters at a rate of up to 100 SLPM, pushing commercial pumps to their limits moving this large quantity of gas without interruption for several years. Two upgrades are considered for XENONnT. A custom high-capacity magnetic piston pump based on the one developed for the EXO200 experiment has been scaled up to support the high demands of this much larger experiment. Additionally, a liquid phase circulation and purification system that purifies the cryogenic liquid directly is being developed, which takes advantage of the much smaller volumetric flow demands of liquid relative to gas. The implementation of both upgrades will be presented. Supported by the National Science Foundation.

  17. Breathing Air Purification for Hyperbaric Purposes, Part II

    Directory of Open Access Journals (Sweden)

    Woźniak Arkadiusz

    2015-03-01

    Full Text Available Determining the efficiency of breathing air purification for hyperbaric purposes with the use of filtration systems is of a crucial importance. However, when the Polish Navy took samples of breathing air from their own filtration plant for quality purposes, these were found to not meet the required standard. The identification of this problem imposed the need to undertake actions aimed at the elimination of the identified disruptions in the process of breathing air production, with the objective of assuring its proper quality. This study presents the results of the initial tests on the air supply sources utilised by the Polish Navy, which were carried out for the purpose of setting a proper direction of future works and implementing corrective measures in order to optimise the breathing air production process. The obtained test results will be used in a subsequent publication devoted to the assessment of the level of efficiency of air purification with the use of a multifaceted approach consisting in the utilisation of various types of air supply sources and different configurations of purification systems.

  18. 可再生纳米复合材料净化网在中央空调系统中的应用探讨%The Applied Discussion of Renewable Nanocomposites Purification Device in the Central Air Conditioning System

    Institute of Scientific and Technical Information of China (English)

    宋宝龙∗; 李振海

    2012-01-01

      The TiO2-activated carbon composite materials with a visible regeneration performancewere prepared, and the development of an air cleaning facility with simple structure, good adsorption performance and low loss according to the adsorption and photocatalytic decomposition of decoupling functions. After measuring resistance of the purification equipment,this paper presented the problems and solutions in the central air conditioning system. Through the experiment and the calculation,this paper deduced the estimates formula of adsorption saturation time, which had important reference value.%  在制备出具有可见光再生性能的纳米 TiO2-活性炭复合材料后,本文根据吸附与光催化分解解耦的原则探讨研制结构简单、具有良好吸附性能、低压损的纳米活性炭空气净化装置。通过实验对净化网的阻力特性进行了探讨,提出了其在中央空调系统中的应用时的问题和解决方法,并根据实验、理论分析,推导出复合材料吸附饱和时间的概算公式,为此复合材料的实际应用提供了重要参考

  19. Assistência circulatória com bomba centrífuga no choque cardiogênico após cirurgia com extracorpórea Assisted circulation for cardiogenic shock following cardiopulmonary bypass with a centrifugal pump

    Directory of Open Access Journals (Sweden)

    Paulo M Pêgo-Fernandes

    1991-08-01

    Full Text Available No período de abril a dezembro de 1990, quatro pacientes foram submetidos a utilização de bomba centrífuga, para suporte circulatório. Em todos, foi colocado previamente balão intra-aórtico e feito uso maciço de drogas vasoativas. A primeira paciente apresentava aneurisma de ventrículo esquerdo, com fração de ejeção de 16% no pré-operatório. Após correção do aneurisma, não se conseguiu retirá-la de extracorpórea pelos métodos convencionais. Optou-se, então, pelo uso de assistência ventricular esquerda, que foi mantida por 48 horas. Teve boa evolução, estando, atualmente, no 11º mês de pós-operatório em classe funcional II. O segundo caso foi de paciente submetido a revascularização do miocárdio e troca valvar mitral. No 2? dia de pós-operatório, apresentou oclusão de ponte de safena para descendente anterior, com infarto e parada cardíaca. Massageado, reaberto e recolocado em circulação extracorpórea, não saiu de "bomba". O ventrículo esquerdo apresentava infarto anterior extenso, sendo colocado em assistência ventricular esquerd como "ponte" para transplante. Após cinco dias de assistência, sem se conseguir doador, apresentou óbito por embolia pulmonar. O terceiro caso foi de paciente com má função ventricular esquerda, submetido a revascularização do miocárdio. Também não se conseguiu retirar de circulação extracorpórea. Foi colocado em assistência ventricular esquerda por 32 horas, quando se conseguiu retirar a bomba centrífuga. Esse paciente apresentou distúrbios severos de coagulação. Apesar de estável hemodinamicamente, houve piora progressiva da função pulmonar, com óbito no 4º dia de pós-operatório. O quarto caso foi de paciente submetido a correção de aneurisma de ventrículo esquerdo e revascularização do miocárdio. Não se conseguiu retirar de circulação extracorpórea, e optado por assistência ventricular esquerda com bomba centrífuga. Apresentou melhora

  20. Application of optically-induced-dielectrophoresis in microfluidic system for purification of circulating tumour cells for gene expression analysis- Cancer cell line model

    Science.gov (United States)

    Chiu, Tzu-Keng; Chou, Wen-Pin; Huang, Song-Bin; Wang, Hung-Ming; Lin, Yung-Chang; Hsieh, Chia-Hsun; Wu, Min-Hsien

    2016-09-01

    Circulating tumour cells (CTCs) in a blood circulation system are associated with cancer metastasis. The analysis of the drug-resistance gene expression of cancer patients’ CTCs holds promise for selecting a more effective therapeutic regimen for an individual patient. However, the current CTC isolation schemes might not be able to harvest CTCs with sufficiently high purity for such applications. To address this issue, this study proposed to integrate the techniques of optically induced dielectrophoretic (ODEP) force-based cell manipulation and fluorescent microscopic imaging in a microfluidic system to further purify CTCs after the conventional CTC isolation methods. In this study, the microfluidic system was developed, and its optimal operating conditions and performance for CTC isolation were evaluated. The results revealed that the presented system was able to isolate CTCs with cell purity as high as 100%, beyond what is possible using the previously existing techniques. In the analysis of CTC gene expression, therefore, this method could exclude the interference of leukocytes in a cell sample and accordingly contribute to higher analytical sensitivity, as demonstrated in this study. Overall, this study has presented an ODEP-based microfluidic system capable of simply and effectively isolating a specific cell species from a cell mixture.

  1. 液化天然气工厂净化系统技术研究%Study LNG Plant Purification System Technology

    Institute of Scientific and Technical Information of China (English)

    曹龙

    2016-01-01

    Describes the characteristics of liquefied natural gas,analyzes the production process and the principle of liquefied natural gas,and from the three bus control systems,security systems,and explosion-proof intelligent temperature control system,we discussed the LNG automation system,with a view to promote the development of liquefied natural gas automation system,improve the utilization of liquefied natural gas to provide reference.%分析了液化天然气的净化,阐述了液化天然气中酸性气体脱除的化学吸收方法和物理吸收方法,以及原料气中水分脱除的低温冷凝法、固体吸附法和膜法,以期为改进液化天然气工厂的净化技术和降低能耗提供参考。

  2. 血液净化联合DNA免疫吸附治疗重症系统性红斑狼疮的效果%Effect of blood purification combined with DNA immunoadsorption in treatment of severe systemic lupus erythematosus

    Institute of Scientific and Technical Information of China (English)

    鱼强; 刘岐焕; 程范军; 刘晓红; 张有顺

    2016-01-01

    Objective To analyze and explore the effect and safety of continuous blood purification combined with DNA immunoadsorp-tion,prednisone tablets and cyclophosphamide in the treatment of severe systemic lupus erythematosus. Methods 80 patients with severe systemic lupus erythematosus treated in our hospital were taken as the research objects and were averagely divided into the experimental group and the con-trol group. The control group were treated with DNA immunoadsorption,while the experimental group were treated with continuous blood purifica-tion combined with DNA immunoadsorption. The curative effect,renal function indexes and antinuclear antibody in the two groups were compara-tively analyzed. Results The total effective rate in the experimental group(95. 00℅ )was significantly higher than that in the control group(62. 50℅ )( P ﹤ 0. 05). 2 months of treatment later,the blood urea nitrogen(5. 31 ± 1. 25)mmol/ L and serum creatinine(98. 58 ± 21. 17)μmol/L of the experimental group were significantly better than those of the control group[(7. 23 ± 1. 33)mmol/ L and(128. 38 ± 25. 76)μmol/ L]( P ﹤ 0. 05). ANA(1. 62 ± 0. 41)and anti - ds - DHA antibody(0. 91 ± 0. 42)in the experimental group were significantly higher than those of the control group[(2. 21 ± 0. 69)and(1. 21 ± 0. 62)]( P ﹤ 0. 05). The incidence of adverse reactions in the experimental group(7. 50℅ ) was significantly lower than that in the control group(27. 50℅ )( P ﹤ 0. 05). Antinuclear antibody test group sensitivity of 30. 00℅ was signifi-cantly lower than the 62. 50℅ in the control group,the difference was statistically significant( P ﹤ 0. 05). Conclusion The effect of blood puri-fication combined with DNA immunoadsorption in treatment of severe systemic lupus erythematosus is significant. It can improve the renal function of patients,safe and reliable. It is worthy of promotion.%目的:探讨连续性血液净化联合 DNA 免疫吸附配合泼尼松片、环磷

  3. Uso de bomba centrífuga no pós-operatório de cirurgia cardíaca Assisted circulation in cardiac surgery

    Directory of Open Access Journals (Sweden)

    Paulo M Pêgo-Fernandes

    1992-12-01

    Full Text Available Nos casos de choque cardiogênico após cirurgia cardíaca com auxílio da circulação extracorpórea retratarlos às drogas e ao balão intra-aórtico, as bombas centrífugas têm sido a primeira opção em vários Serviços. Esse fato deve-se à facilidade de manuseio, de instalação, custo razoável, grande disponibilidade, e alto grau de eficiência. O objetivo deste trabalho é o relato da experiência do Instituto do Coração com 8 pacientes submetidos a essa terapêutica, no período de maio de 1990 a dezembro de 1991. Nesses 8 doentes foi utilizado previamente balão intra-aórtico e feito uso maciço de drogas vasoativas. A idade variou de 54 a 66 anos. Três foram submetidos a revascularização do miocárdio, 2 a correção de aneurisma de ventrículo esquerdo, 2 a troca de valva mitral e 1 a transplante cardíaco. Em 7 a assistência foi de ventrículo esquerdo e em 1 de direito. A duração da assistência variou de 18 a 126 horas. Ocorreram três óbitos em assistência, sendo que dois eram pacientes em "ponte" para transplante que não obtiveram doador, e um morreu por complicações de sangramento e insuficiência renal aguda. Dos 5 (62,5% pacientes retirados da assistência, 2 faleceram tardiamente sendo 1 com pulmão de choque e 1 com complicação neurológica e insuficiência renal. Quando comparamos a evolução clínica com o pico de CKMB, verificamos que os 3 pacientes com pico maior que 80 faleceram, 2 em assistência e 1 tardiamente. Dos 5 doentes com pico de CKMB menor que 80, 4 foram retirados de assistência, com 3 sobreviventes tardios, e o único que morreu em assistência foi por problemas de sangramento. Os 3 (35% pacientes sobreviventes estão no 5º, 9º e 19º meses de seguimento, 2 em classe funcional II e 1 em classe I. A utilização precoce, antes de complicações irreversíveis, da assistência circulatória, deverá permitir resultados progressivamente melhores, nesse grupo de pacientes de alto risco

  4. Gas purification process. Verfahren zur Gasreinigung

    Energy Technology Data Exchange (ETDEWEB)

    Hoelter, H.; Gresch, H.; Igelbuescher, H.; Dewert, H.

    1987-08-13

    To avoid the problems of reheating in a wet process as well as the problems of higher gas supply in a dry process, the invention proposes to separate the raw gas in two component currents, one of which undergoes wet purification while the other is led through a dry purification process. The two component currents are mixed before entering the stack. The dry chemisorption masses added in substoichiometric doses are treated in a milk-of-lime processing stage, after which the reacted and non-reacted chemisorption masses are treated by wet purification and then by oxidation.

  5. Industrial-scale production and purification of a heterologous protein in Lactococcus lactis using the nisin-controlled gene expression system NICE: The case of lysostaphin

    Directory of Open Access Journals (Sweden)

    Floris Esther

    2005-05-01

    Full Text Available Abstract Background The NIsin-Controlled gene Expression system NICE of Lactococcus lactis is one of the most widespread used expression systems of Gram-positive bacteria. It is used in more than 100 laboratories for laboratory-scale gene expression experiments. However, L. lactis is also a micro-organism with a large biotechnological potential. Therefore, the aim of this study was to test whether protein production in L. lactis using the NICE system can also effectively be performed at the industrial-scale of fermentation. Results Lysostaphin, an antibacterial protein (mainly against Staphylococcus aureus from S. simulans biovar. Staphylolyticus, was used as a model system. Food-grade lysostaphin expression constructs in L. lactis were grown at 1L-, 300-L and 3000-L scale and induced with nisin for lysostaphin production. The induction process was equally effective at all scales and yields of about 100 mg/L were obtained. Up-scaling was easy and required no specific effort. Furthermore, we describe a simple and effective way of downstream processing to obtain a highly purified lysostaphin, which has been used for clinical phase I trials. Conclusion This is the first example that shows that nisin-regulated gene expression in L. lactis can be used at industrial scale to produce large amounts of a target protein, such as lysostaphin. Downstream processing was simple and in a few steps produced a highly purified and active enzyme.

  6. Application of biocompounds for the purification of regenerates containing nitrogen taken from condensate cleaning systems; Einsatz von Biocompounds zur Reinigung stickstoffhaltiger Regenerate aus Kondensatreinigungsanlagen (KRA)

    Energy Technology Data Exchange (ETDEWEB)

    Walter, R.; Buesching, K.; Boettger, C.; Oswald, N. [HS Merseburg (F.H.) (Germany). Fachbereich Ingenieur- und Naturwissenschaften; Ochmann, C. [VertUm GmbH, Markkleeberg (Germany)

    2008-07-01

    Using the Biocompound technique, it is possible to biologically purify the regenerates of condensate cleaning systems to ensure direct discharger quality. In this process simultaneous nitrification and denitrification can take place temporally and locally using a completely aerated fluidised bed reactor. The biofilm carriers used for this purpose contain two different readily bio-degradable bio-polymers, the so-called Biocompounds. (orig.)

  7. Microbial polyhydroxyalkanote synthesis repression protein PhaR as an affinity tag for recombinant protein purification

    Directory of Open Access Journals (Sweden)

    Chen Guo

    2010-05-01

    Full Text Available Abstract Background PhaR which is a repressor protein for microbial polyhydroxyalkanoates (PHA biosynthesis, is able to attach to bacterial PHA granules in vivo, was developed as an affinity tag for in vitro protein purification. Fusion of PhaR-tagged self-cleavable Ssp DnaB intein to the N-terminus of a target protein allowed protein purification with a pH and temperature shift. During the process, the target protein was released to the supernatant while PhaR-tagged intein was still immobilized on the PHA nanoparticles which were then separated by centrifugation. Results Fusion protein PhaR-intein-target protein was expressed in recombinant Escherichia coli. The cell lysates after sonication and centrifugation were collected and then incubated with PHA nanoparticles to allow sufficient absorption onto the PHA nanoparticles. After several washing processes, self-cleavage of intein was triggered by pH and temperature shift. As a result, the target protein was released from the particles and purified after centrifugation. As target proteins, enhanced green fluorescent protein (EGFP, maltose binding protein (MBP and β-galactosidase (lacZ, were successfully purified using the PhaR based protein purification method. Conclusion The successful purification of EGFP, MBP and LacZ indicated the feasibility of this PhaR based in vitro purification system. Moreover, the elements used in this system can be easily obtained and prepared by users themselves, so they can set up a simple protein purification strategy by themselves according to the PhaR method, which provides another choice instead of expensive commercial protein purification systems.

  8. Entanglement purification of unknown quantum states

    Science.gov (United States)

    Brun, Todd A.; Caves, Carlton M.; Schack, Rüdiger

    2001-04-01

    A concern has been expressed that ``the Jaynes principle can produce fake entanglement'' [R. Horodecki et al., Phys. Rev. A 59, 1799 (1999)]. In this paper we discuss the general problem of distilling maximally entangled states from N copies of a bipartite quantum system about which only partial information is known, for instance, in the form of a given expectation value. We point out that there is indeed a problem with applying the Jaynes principle of maximum entropy to more than one copy of a system, but the nature of this problem is classical and was discussed extensively by Jaynes. Under the additional assumption that the state ρ(N) of the N copies of the quantum system is exchangeable, one can write down a simple general expression for ρ(N). By measuring one or more of the subsystems, one can gain information and update the state estimate for the remaining subsystems with the quantum version of the Bayes rule. Using this rule, we show how to modify two standard entanglement purification protocols, one-way hashing and recurrence, so that they can be applied to exchangeable states. We thus give an explicit algorithm for distilling entanglement from an unknown or partially known quantum state.

  9. Purification

    DEFF Research Database (Denmark)

    Andersen, Astrid Oberborbeck

    2017-01-01

    —was treated before it was returned to the river where it continues its flow downstream towards cultivated fields and, finally, into the Pacific Ocean. It takes specialized knowledge and manifold technologies to manage water and sustain life in Arequipa, and engineers are central actors for making water flow......In Arequipa, Peru’s second largest city, engineers work hard to control water flows and provide different sectors with clean and sufficient water. In 2011, only 10 percent of the totality of water used daily by Arequipa’s then close to 1 million people—in households, tourism, industry, and mining...

  10. 转炉烟气净化控制系统的设计%Design of Flue Gas Purification Control System of Converter

    Institute of Scientific and Technical Information of China (English)

    李伟明; 胡文发

    2012-01-01

    介绍西门子S7—400PLC和WINCC组态软件在转炉干法除尘控制系统中的应用,给出相应的控制策略,设计程序模块。%The application of Siemens S7-400 PLC and WINCC configuration software used in dry dedusting control system is introduced. The control strategy is given and program module is designed.

  11. Design of an integrated piggery system with recycled water, biomass production and water purification by vermiculture, macrophyte ponds and constructed wetlands.

    Science.gov (United States)

    Morand, Philippe; Robin, Paul; Pourcher, Anne-Marie; Oudart, Didier; Fievet, Sebastien; Luth, Daniel; Cluzeau, Daniel; Picot, Bernadette; Landrain, Brigitte

    2011-01-01

    Since 2001 the swine experimental station of Guernévez has studied biological treatment plants for nutrient recovery and water recycling, suited to the fresh liquid manure coming out of flushing systems. An integrated system with continuous recycling was set up in 2007, associated with a piggery of 30 pregnant sows. It includes a screen, a vermifilter, and macrophyte ponds alternating with constructed wetlands. The screen and the vermifilter had a lower removal efficiency than in previous studies on finishing pigs. A settling tank was then added between the vermifilter and the first lagoon to collect the worm casts. A second vermifilter was added to recover this particulate organic matter. A storage lagoon was added to compensate for evaporative losses and complete pollution abatement, with goldfish as a bioindicator of water quality. The removal efficiency of the whole system was over 90% for COD and nitrogen, over 70% for phosphorus and potassium, and more than 4 logarithmic units for pathogens (E. coli, enterococci, C perfringens). Plant production was about 20 T DM ha(-1) y(-1). Floating macrophytes (Azolla caroliniana, Eichhornia crassipes, Hydrocotyle vulgaris) were more concentrated in nutrients than helophytes (Phragmites australis, Glyceria aquatica,…). Azolla caroliniana was successfully added to feed finishing pigs.

  12. Construction of high level prokaryotic expression and purification system of PD-L1 extracellular domain by using Escherichia coli host cell machinery.

    Science.gov (United States)

    Kalim, Muhammad; Chen, Jie; Wang, Shenghao; Lin, Caiyao; Ullah, Saif; Liang, Keying; Ding, Qian; Chen, Shuqing; Zhan, Jinbiao

    2017-10-01

    Programmed cell death 1 ligand 1 (PD-L1) is a trans-membrane protein highly expressed on the membrane of cancer cell, which binds inhibitory receptor of PD-1 on the T cells and attenuates anti-tumor immune response.The strategy of blocking PD1 and PD-L1 interaction has been widely used for anti-cancer drug development. The DNA encoding extracellular domain of PD-L1 was cloned and expressed with the pET30(+) and Escherichia coli BL21(DE3) system. Cloning of PD-L1 extracellular domain was confirmed by PCR and enzymatic digestion. Sequence analysis of cloned targeted genes showed 100% homology of original sequence. The recombinant protein was expressed using 1mM/mL IPTG and purified by affinity chromatography on a column of Ni-NTA and confirmed by SDS-PAGE and western blot analysis. Results showed that our constructed pET30(+)/PDL1-ECD system efficiently produces desired recombinant protein with molecular weight of 38.1kDa. The prokaryotic expression system provides an easy method to express PD-L1 extracellular domain that further facilitate the role of PD-1/PD-L1 binding inhibition and helps in valuable drug and antibodies production. Copyright © 2017 European Federation of Immunological Societies. Published by Elsevier B.V. All rights reserved.

  13. Liquid membrane purification of biogas

    Energy Technology Data Exchange (ETDEWEB)

    Majumdar, S.; Guha, A.K.; Lee, Y.T.; Papadopoulos, T.; Khare, S. (Stevens Inst. of Tech., Hoboken, NJ (United States). Dept. of Chemistry and Chemical Engineering)

    1991-03-01

    Conventional gas purification technologies are highly energy intensive. They are not suitable for economic removal of CO{sub 2} from methane obtained in biogas due to the small scale of gas production. Membrane separation techniques on the other hand are ideally suited for low gas production rate applications due to their modular nature. Although liquid membranes possess a high species permeability and selectivity, they have not been used for industrial applications due to the problems of membrane stability, membrane flooding and poor operational flexibility, etc. A new hollow-fiber-contained liquid membrane (HFCLM) technique has been developed recently. This technique overcomes the shortcomings of the traditional immobilized liquid membrane technology. A new technique uses two sets of hydrophobic, microporous hollow fine fibers, packed tightly in a permeator shell. The inter-fiber space is filled with an aqueous liquid acting as the membrane. The feed gas mixture is separated by selective permeation of a species through the liquid from one fiber set to the other. The second fiber set carries a sweep stream, gas or liquid, or simply the permeated gas stream. The objectives (which were met) of the present investigation were as follows. To study the selective removal of CO{sub 2} from a model biogas mixture containing 40% CO{sub 2} (the rest being N{sub 2} or CH{sub 4}) using a HFCLM permeator under various operating modes that include sweep gas, sweep liquid, vacuum and conventional permeation; to develop a mathematical model for each mode of operation; to build a large-scale purification loop and large-scale permeators for model biogas separation and to show stable performance over a period of one month.

  14. The modified swirl sedimentation tanks for water purification.

    Science.gov (United States)

    Ochowiak, Marek; Matuszak, Magdalena; Włodarczak, Sylwia; Ancukiewicz, Małgorzata; Krupińska, Andżelika

    2017-03-15

    This paper discusses design, evaluation, and application for the use of swirl/vortex technologies as liquid purification system. A study was performed using modified swirl sedimentation tanks. The vortex separators (OW, OWK, OWR and OWKR) have been studied under laboratory conditions at liquid flow rate from 2.8⋅10(-5) to 5.1⋅10(-4) [m(3)/s]. The pressure drop and the efficiency of purification of liquid stream were analyzed. The suspended particles of different diameters were successfully removed from liquid with the application of swirl chambers of proposed constructions. It was found that damming of liquid in the tank increases alongside liquid stream at the inlet and depends on the tank construction. The efficiency of the sedimentation tanks increases alongside the diameters of solid particles and decrease in the liquid flow rate. The best construction proved to be the OWR sedimentation tank due to smallest liquid damming, even at high flow rates, and the highest efficiency of the purification liquid stream for solid particles of the smallest diameter. The proposed solution is an alternative to the classical constructions of sedimentation tanks.

  15. [Separation and purification of cellulase using affinity membrane].

    Science.gov (United States)

    Shi, Xiang-zhu; Guo, Chun-teng; Zhou, Jian-wu; Wang, Zhong-lai; Rao, Ping-fan

    2002-07-01

    The importance of cellulase as a means for the efficient utilization of abundant cellulose resources in the world has been well recognized. Many researchers devote themselves to studying the mechanism of the action of cellulase to cellulose so that such expensive enzyme can be used much more widely. The first step is to obtain cellulase of high purity. So purification of cellulase is the key point in this field. However, the major problem in isolation is that cellulase is a complicated enzyme system and needs too many steps for separation, and that every cellulase needs special purification processing which cannot be used for the others. A novel method for the separation of the cellulase from crude extraction of Aspergillus niger with normal qualitative filter paper processed by 5 mol/L sodium hydroxide without precipitation and desalting steps was developed. Further purification of the cellulase was achieved by using an anion-exchange column of POROS 20HQ. The cellulase purified was identified as a new endoglucanase that had relatively high endurance to pH and temperature. Its relative molecular mass was estimated to be 60,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. This enzyme exhibited very high activity towards carboxymethyl cellulose (CMC) with specific activity of 350 U.mg-1 and the recovery of activity of 9.7%. Its optimum pH and temperature were 4.0 and 70 degrees C, respectively. This is a simple, rapid and efficient method for purifying cellulase with high activity.

  16. [The cloning, expression, purification and immunological identification of wild-type and mutant hepatitis B virus X gene in pGEX-6P-2 system].

    Science.gov (United States)

    Zhang, Xiao-Hui; Wang, Chang-Yuan; Tan, Bing-Qin; Li, Cheng; Du, Lei; Wang, Yong-Kang; Zhang, Hong-Hua; Dong, Ge-Feng

    2011-09-01

    To settle the foundation for the future research on the influence of wild and mutant (A1762T/ G1764A) HBV X gene on the progress of chronic HBV infection and hepatic tumorigenicity, wild and mutant (A1762T/G1764A) HBxAgs expression system was constructed. The wild and mutant (A1762T/ G1764A) HBV X genes were amplified with polymerase chain reaction (PCR) from HBV genome were inserted into pGEX-6P-2 and confirmed by sequencing respectively. Prokaryotic expression vectors pGEX-6P-2-hbvx(w) and pGEX-6P-2-hbvx(m) (A1762T/G1764A) were constructed and transformed to Trans1-blue; wild and mutant HBxAgs were expressed through IPTG induction respectively; after refolding of inclusion body, the wild and mutant HBxAgs were purified with GSTrap FF; and analysised by SDS-PAGE, Western blot and ELISA. SDS-PAGE analysis showed that the expression system was able to express target protein efficiently; the concentrations of purified wild HBxAg and mutant HBxAg were 4.88 mg/mL and 5.07 mg/mL respectively; Western blot analysis certified both the wild HBxAg and the mutant HBxAg could be recognized by the same monoclonal antibody against HBxAg; the two expressed fusion antigens coated in microtiter plate were able to react with the sera of HBV infected patients but not with the sera from healthy donors in ELISA. Results demonstrated that we successfully established a system for expression of hepatitis B x antigen and lay the foundation for further research on the role and molecular mechanisms of the mutant HBxAg in the progress of chronic HBV infection and hepatic tumorigenicity.

  17. Isolation, production, purification, assay and characterization of ...

    African Journals Online (AJOL)

    Isolation, production, purification, assay and characterization of fibrinolytic ... are isolated from Bacillus subtilis, β-haemolytic Streptococci and urine sample. ... recombinant E.coli containing short fragment genomic DNA of Pseudomonas sp.

  18. Purification of rhamnolipid using colloidal magnetic nanoparticles

    African Journals Online (AJOL)

    STORAGESEVER

    2009-07-06

    Jul 6, 2009 ... separation and purification of bio-molecules, particularly, .... of model Rhamnolipid by ion exchange processes. The ..... dynamic diameter of the particles is 35.6 nm. ... solubility of rhaminolipid was decreased and amount of.

  19. Accelerated purification of colloidal silica sols

    Science.gov (United States)

    Bahnsen, E. B.; Garofalini, S.; Pechman, A.

    1979-01-01

    Accelerated purification process for colloidal sols using heat/deionization scheme, sharply reduces waiting time between deionization cycles from several months to a few days. Process produces same high purity silica sols as conventional methods.

  20. Affinity purification of recombinant human plasminogen activator ...

    African Journals Online (AJOL)

    Purpose: To develop processes for effective isolation and purification of recombinant human plasminogen ... three hybridoma strains were superior for producing PR-mAbs (C1, C4, C8). ..... characterization of a polyol- responsive monoclonal.

  1. Um circuito simples com bomba única para circulação extracorpórea com oxigenação autógena A simple circuit with only centrifugal pump for extracorporeal circulation with autogenous oxygenation

    Directory of Open Access Journals (Sweden)

    Euclydes MARQUES

    2002-06-01

    Full Text Available MATERIAL E MÉTODOS: Foi testado em 30 cães um circuito capaz de promover circulação extracorpórea (CEC com oxigenação autógena (OA do sangue, usando apenas uma bomba centrífuga. Esta montagem dispensou bombeamento para o lado direito: o gradiente de pressão bastante para vencer a resistência arterial pulmonar foi vencido aumentando-se a pressão nas artérias pulmonares pela expansão da volemia e diminuindo-se a pressão do átrio esquerdo pela drenagem dessa câmara mediante um sifão. O coração foi mantido em ritmo de fibrilação ventricular durante o período de perfusão e ao seu término, o ritmo próprio foi recuperado mediante cardioversão elétrica. RESULTADOS: Este circuito permitiu a manutenção de parâmetros hemodinâmicos e gases sangüíneos adequados durante a perfusão. O campo operatório e a mobilidade do coração foram similares aos proporcionados pela CEC convencional. CONCLUSÃO: Concluímos que o uso de bomba centrífuga única simplifica a OA, podendo tornar-se uma escolha prática nos procedimentos de revascularização do miocárdio.It was tested in 30 dogs a circuit capable to allow extracorporeal circulation (ECC with autogenous oxygenation (AO of the blood employing an only centrifugal pump. With this assembly is unnecessary a pump to the right side: the gradient of transpulmonary pressure was obtained by increasing the pulmonary artery pressure by volemic expansion and decreasing the left atrial pressure by draining this camera by means of a siphon. The heart was electrically fibrillated in the beginning of the bypass and defibrillated in the end. This circuit allowed the maintenance of normal hemodynamic parameters and normal blood gases level during ECC. The operative field and the mobility of the heart were similar to those provided by conventional CEC. We concluded that the use of an only centrifugal pump simplifies ECC with AO, turning it a practical choice for the procedures of myocardial

  2. Expression, Purification, and Characterization of a Sucrose Nonfermenting 1-Related Protein Kinases 2 of Arabidopsis thaliana in E. coli-Based Cell-Free System

    Directory of Open Access Journals (Sweden)

    Xu Zhang

    2016-01-01

    Full Text Available The plant-specific sucrose nonfermenting 1-related protein kinase 2 (SnRK2 family is considered an important regulator of plant responses to abiotic stresses such as drought, cold, salinity, and nutrition deficiency. However, little information is available on how SnRK2s regulate sulfur deprivation responses in Arabidopsis. Large-scale production of SnRK2 kinases in vitro can help to elucidate the biochemical properties and physiological functions of this protein family. However, heterogenous expression of SnRK2s usually leads to inactive proteins. In this study, we expressed a recombinant Arabidopsis SnRK2.1 in a modified E. coli cell-free system, which combined two kinds of extracts allowing for a convenient and affordable protein preparation. The recombinant SnRK2.1 was produced in large-scale and the autophosphorylation activity of purified SnRK2.1 was characterized, allowing for further biochemical and substrate binding analysis in sulfur signaling. The application of this improved E. coli cell-free system provides us a promising and convenient platform to enhance expression of the target proteins economically.

  3. Remote monitoring of primary coolant system centrifugal pump of RA-6 reactor; Monitoreo remoto de la bomba centrifuga primaria del reactor RA-6

    Energy Technology Data Exchange (ETDEWEB)

    Garcia Peyrano, Oscar [Comision Nacional de Energia Atomica, Rio Negro (Argentina). Centro Atomico Bariloche. Lab. Vibraciones; Rico, Nestor [Comision Nacional de Energia Atomica, Rio Negro (Argentina). Centro Atomico Bariloche. Reactor RA-6

    1996-07-01

    From the beginning of the Industrialization, the techniques of maintenance went evolving, generation the repairs (Corrective Maintenance), being their costs extremely high or unknown by the management. When the circumstances determined the mount of costs and the heavy that these was for the budgets of Production, the new Preventive Maintenance triggered the mechanism of repairs or change the spares in base to statistical and experiences along the years. But the reality of the costs made shade in the budgets for area, because they were changed and they threw element still useful. A new technique that has certain cogent characteristics, such as Not destructive Economic and Comfortable for the personnel: of production, maintenance, quality control and production control in a preventive form between the different areas being all they convinced of the problem in the component and not in a frequency of change. The Predictive Maintenance had been born decided to be utilized for all the leader companies at the beginning and for the majority of the medium. The success obtained was because of the rotation costs, their speed of diagnosis, anticipating the replacement outside of service of their components or equipment and trust that generate this activity in the personnel. (author)

  4. Extracorporeal blood purification in burns: a review.

    Science.gov (United States)

    Linden, Katharina; Stewart, Ian J; Kreyer, Stefan F X; Scaravilli, Vittorio; Cannon, Jeremy W; Cancio, Leopoldo C; Batchinsky, Andriy I; Chung, Kevin K

    2014-09-01

    A prolonged and fulminant inflammatory state, with high levels of pro- and anti-inflammatory mediators, is seen after extensive thermal injury. Blood purification techniques including plasma exchange, continuous venovenous hemofiltration, and adsorbing membranes have the potential to modulate this response, thereby improving outcomes. This article describes the scientific rationale behind blood purification in burns and offers a review of literature regarding its potential application in this patient cohort.

  5. Selection of technical and economic purification system wastewater, applying the assessment of the water decontamination; Seleccion tecnico-economica del sistema de depuracion de aguas residuales

    Energy Technology Data Exchange (ETDEWEB)

    Rodriguez Miranda, J. P.

    2009-07-01

    The decontamination in water bodies can be realised among others by treatments at the end of the tube. For it is necessary to apply to a methodology or procedure for economic the technical selection of alternatives for the handling or treatments of liquid residues at the end of the tube. Calculation example, a study of case (municipality) in the Savannah of Bogota was developed (Colombia), where the technical valuation of the best alternative of treatment at the end of the tube was observed that stops the case of the analysis, the optimal system is the percolating filter from the plant of comparison of removal efficiencies, costs of investment, operation and maintenance, as well as the specific qualitative valuation of each alternative. (Author) 27 refs.

  6. 一种净化水质的复合生物修复系统%A composite bioremediation system for water purification

    Institute of Scientific and Technical Information of China (English)

    袁亚光; 张弛; 谢卫平; 赵福庚; 钦佩

    2012-01-01

    在实验室内利用人工模拟方法,选择水蕹菜(Ipomoea aquatica)、泥鳅(Misgurus anguillicaudatus)、沼泽红假单胞菌(Rhodopseudomonas palustris)为工程物种,构建一套水生经济植物-水生动物-微生物复合生物修复系统进行污水修复,研究该系统中动植物生物量及水质指标的变化.结果表明,在23 d的实验周期中,水体铵态氮(NH4+-N)下降96.5%,硝态氮(NO3--N)下降82.2%,总磷(TP)下降53.2%,化学需氧量(CODMn)下降24.5%.水蕹菜平均增重31.2%,泥鳅平均增重6.1%.这种复合的生物修复模式具有较好的经济效益与环境效益.%By the method of artificial simulation in laboratory, and with water spinach (Ipomoea aquatic), loach (Misgurus anguillkaudatus) , and a kind of pseudomonad (Rhodopseudomonas palustris) as the engineering species, a composite bioremediation system of aquatic economic plant - aquatic animal - microorganism was constructed to improve the water quality of sewage. The biomass of I. aquatic and M. anguillicaudatus in the system and the water quality indices were investigated. Within the 23 days experimental period, the nutrient concentrations in the sewage decreased significantly, with the removal rates of NH4+-N, NO3--N, TP, and CODMn being 96.5%, 82. 2%, 53. 2%, and 24.5%, respectively. Meanwhile, the biomass of I. aquatic and M. anguillicaudatus was averagely increased by 31.2% and 6. 1% , respectively. All the results suggested that this composite bioremediation model had good economic and environmental benefits.

  7. Overcoming inefficient secretion of recombinant VEGF-C in baculovirus expression vector system by simple purification of the protein from cell lysate.

    Science.gov (United States)

    Klaus, Tomasz; Kulesza, Małgorzata; Bzowska, Monika; Wyroba, Barbara; Kilarski, Witold W; Bereta, Joanna

    2015-06-01

    The first reports about successfully expressed recombinant proteins with the use of a baculovirus vector were published over 30years ago. Despite the long time of refining this expression system, early problems with the production of baculovirus-derived secretory proteins are still not satisfactorily solved. The high expression level driven by baculoviral promoters often does not result in the desired yield of secreted recombinant proteins, which frequently accumulate inside insect cells and are only partially processed. During our attempts to produce vascular endothelial growth factor C (VEGF-C) with the use of a baculovirus vector we also faced an inefficient secretion of the recombinant protein to culture medium. We were not able to improve the outcome and obtain an acceptable concentration of VEGF-C in the medium by changing the culture conditions or utilizing different signal peptides. However, as a significant amount of native VEGF-C was detected inside the baculovirus-infected cells, we developed a simple method to purify recombinant, glycosylated VEGF-C from a lysate of the cells. The presented results indicate that the lack of a secretory protein in the insect cell culture medium after baculovirus infection does not necessarily signify failure in the production of the protein. As demonstrated by us and contrary to generally accepted views, the lysate of baculovirus-infected cells may constitute a valuable source of the biologically active, secretory protein.

  8. 循环流水产养殖系统净化研究%Research on Purification of Recirculating Aquaculture System

    Institute of Scientific and Technical Information of China (English)

    汪辉; 马伟芳; 郭浩; 曾凡刚; 韩冬梅; 丁志伟

    2012-01-01

    文章研究了不同水力停留时间和壳聚糖的投加对循环流水产养殖系统的影响,结果表明,当水力停留时间为8h,系统内除磷,除氨氮、COD效果明显,通过生物滤池和植物滤池的去除,NO2^-浓度下降,植物滤池平均出水浓度总磷1.60mg/L、氨氮0.016mg/L、COD为21.788mg/L;养鱼池投加壳聚糖,出水的色度和浊度都明显降低。%This paper studied the influence of different hydraulic retention time and chitosan additive on recirculating aquaculture system. The results show that, when the hydraulic retention time was 8 h, the removal efficiency of phosphorus, ammonia nitrogen and COD was high. Through the bio - filter and plants filter, the NO2^- concentration dropped. In the effluent of plant filter, the average concentration of phosphorus, ammonia nitrogen and COD was 1.60 mg/L, 0. 016 mg/L and 21. 788 rag/L, respectively. The turbidity and chromaticity were obviously reduced by dosing ehitosan in the fish pond.

  9. Proteins from rat liver cytosol which stimulate mRNA transport. Purification and interactions with the nuclear envelope mRNA translocation system.

    Science.gov (United States)

    Schröder, H C; Rottmann, M; Bachmann, M; Müller, W E; McDonald, A R; Agutter, P S

    1986-08-15

    Two polysome-associated proteins with particular affinities for poly(A) have been purified from rat liver. These proteins stimulate the efflux of mRNA from isolated nuclei in conditions under which such efflux closely stimulates mRNA transport in vivo, and they are therefore considered as mRNA-transport-stimulatory proteins. Their interaction with the mRNA-translocation system in isolated nuclear envelopes has been studied. The results are generally consistent with the most recently proposed kinetic model of mRNA translocation. One protein, P58, has not been described previously. It inhibits the protein kinase that down-regulates the NTPase, it enhances the NTPase activity in both the presence and the absence of poly(A) and it seems to increase poly(A) binding in unphosphorylated, but not in phosphorylated, envelopes. The other protein, P31, which probably corresponds to the 35,000-Mr factor described by Webb and his colleagues, enhances the binding of poly(A) to the mRNA-binding site in the envelope, thus stimulating the phosphoprotein phosphatase and, in consequence, the NTPase. The possible physiological significance of these two proteins is discussed.

  10. Unified Model of Purification Units in Hydrogen Networks

    Institute of Scientific and Technical Information of China (English)

    吴思东; 王彧斐; 冯霄

    2014-01-01

    Purification processes are widely used in hydrogen networks of refineries to increase hydrogen reuse. In refineries, hydrogen purification techniques include hydrocarbon, hydrogen sulfide and CO removal units. In addi-tion, light hydrocarbon recovery from the hydrogen source streams can also result in hydrogen purification. In order to simplify the superstructure and mathematical model of hydrogen network integration, the models of different pu-rification processes are unified in this paper, including mass balance and the expressions for hydrogen recovery and impurity removal ratios, which are given for all the purification units in refineries. Based on the proposed unified model, a superstructure of hydrogen networks with purification processes is constructed.

  11. 纯化水系统臭氧消毒方法的研究%Study of Ozone Disinfection of Purification Water System

    Institute of Scientific and Technical Information of China (English)

    韩冬; 安爱军

    2013-01-01

    Objective To study the bactericidal effect of ozone on staphylococcus aureus and disinfection ability of purified water storage tank and pipeline. Methods 0.98 mg/L ozone solution is prepared with JW-5 water treatment ozone generator, and its bactericidal effects on staphylococcus aureus and disinfection effect on purified water storage tank and pipeline are measured. The sample, which is quantification ally trained through membrane filtration-agar culture, is taken at different time to calculate sterilization rate. Results The sterilization rates of ozone solution against staphylococcus aureus in 5 min, 10 min, 30 min are 99.29%, 100%, 100%. The sterilization rates for purified water pipeline and storage tank in 10 min, 45 min and 60 min are 95.79%, 99.85% and 100%. Conclusion Ozone disinfection is an exact method in the course of purifying water system disinfection.%目的 研究臭氧对金黄色葡萄球菌的杀菌效果和对纯化水贮罐和管道的消毒能力.方法利用JW-5型水处理臭氧发生器制备浓度为0.98 mg/L的臭氧溶液,分别对金黄色葡萄球菌杀菌,对纯化水贮罐及管道消毒,在不同时间取样,经薄膜过滤-琼脂培养法定量培养,计算杀菌率.结果臭氧溶液对金葡菌作用5 min、10 min、30 min后,对金葡菌的杀菌率分别为99.29%、100%、100%;对纯化水管道和贮罐冲洗消毒10 min、45 min和60 min,杀菌率分别为95.79%、99.85%和100%.结论臭氧消毒效果确切,能满足纯化水贮罐和管道的消毒要求.

  12. Argon Collection And Purification For Proliferation Detection

    Energy Technology Data Exchange (ETDEWEB)

    Achey, R. [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL); Hunter, D. [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL)

    2015-10-09

    In order to determine whether a seismic event was a declared/undeclared underground nuclear weapon test, environmental samples must be taken and analyzed for signatures that are unique to a nuclear explosion. These signatures are either particles or gases. Particle samples are routinely taken and analyzed under the Comprehensive Nuclear-Test-Ban Treaty Organization (CTBTO) verification regime as well as by individual countries. Gas samples are analyzed for signature gases, especially radioactive xenon. Underground nuclear tests also produce radioactive argon, but that signature is not well monitored. A radioactive argon signature, along with other signatures, can more conclusively determine whether an event was a nuclear test. This project has developed capabilities for collecting and purifying argon samples for ultra-low-background proportional counting. SRNL has developed a continuous gas enrichment system that produces an output stream containing 97% argon from whole air using adsorbent separation technology (the flow diagram for the system is shown in the figure). The vacuum swing adsorption (VSA) enrichment system is easily scalable to produce ten liters or more of 97% argon within twelve hours. A gas chromatographic separation using a column of modified hydrogen mordenite molecular sieve has been developed that can further purify the sample to better than 99% purity after separation from the helium carrier gas. The combination of these concentration and purification systems has the capability of being used for a field-deployable system for collecting argon samples suitable for ultra-low-background proportional counting for detecting nuclear detonations under the On-Site Inspection program of the CTBTO verification regime. The technology also has applications for the bulk argon separation from air for industrial purposes such as the semi-conductor industry.

  13. Purification and characterization of polyphenol oxidase from waste potato peel by aqueous two-phase extraction.

    Science.gov (United States)

    Niphadkar, Sonali S; Vetal, Mangesh D; Rathod, Virendra K

    2015-01-01

    Potato peel from food industrial waste is a good source of polyphenol oxidase (PPO). This work illustrates the application of an aqueous two-phase system (ATPS) for the extraction and purification of PPO from potato peel. ATPS was composed of polyethylene glycol (PEG) and potassium phosphate buffer. Effect of different process parameters, namely, PEG, potassium phosphate buffer, NaCl concentration, and pH of the system, on partition coefficient, purification factor, and yield of PPO enzyme were evaluated. Response surface methodology (RSM) was utilized as a statistical tool for the optimization of ATPS. Optimized experimental conditions were found to be PEG1500 17.62% (w/w), potassium phosphate buffer 15.11% (w/w), and NaCl 2.08 mM at pH 7. At optimized condition, maximum partition coefficient, purification factor, and yield were found to be 3.7, 4.5, and 77.8%, respectively. After partial purification of PPO from ATPS, further purification was done by gel chromatography where its purity was increased up to 12.6-fold. The purified PPO enzyme was characterized by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), followed by Km value 3.3 mM, and Vmax value 3333 U/mL, and enzyme stable ranges for temperature and pH of PPO were determined. These results revealed that ATPS would be an attractive option for obtaining purified PPO from waste potato peel.

  14. Extraction and purification methods in downstream processing of plant-based recombinant proteins.

    Science.gov (United States)

    Łojewska, Ewelina; Kowalczyk, Tomasz; Olejniczak, Szymon; Sakowicz, Tomasz

    2016-04-01

    During the last two decades, the production of recombinant proteins in plant systems has been receiving increased attention. Currently, proteins are considered as the most important biopharmaceuticals. However, high costs and problems with scaling up the purification and isolation processes make the production of plant-based recombinant proteins a challenging task. This paper presents a summary of the information regarding the downstream processing in plant systems and provides a comprehensible overview of its key steps, such as extraction and purification. To highlight the recent progress, mainly new developments in the downstream technology have been chosen. Furthermore, besides most popular techniques, alternative methods have been described.

  15. A high-yield co-expression system for the purification of an intact drs2p-cdc50p lipid flippase complex, critically dependent on and stabilized by phosphatidylinositol-4-phosphate

    DEFF Research Database (Denmark)

    Azouaoui, Hassina; Montigny, Cédric; Ash, Miriam-Rose

    2014-01-01

    P-type ATPases from the P4 subfamily (P4-ATPases) are energy-dependent transporters, which are thought to establish lipid asymmetry in eukaryotic cell membranes. Together with their Cdc50 accessory subunits, P4-ATPases couple ATP hydrolysis to lipid transport from the exoplasmic to the cytoplasmic...... leaflet of plasma membranes, late Golgi membranes, and endosomes. To gain insights into the structure and function of these important membrane pumps, robust protocols for expression and purification are required. In this report, we present a procedure for high-yield co-expression of a yeast flippase......, the Drs2p-Cdc50p complex. After recovery of yeast membranes expressing both proteins, efficient purification was achieved in a single step by affinity chromatography on streptavidin beads, yielding ∼1-2 mg purified Drs2p-Cdc50p complex per liter of culture. Importantly, the procedure enabled us to recover...

  16. 罗氏沼虾育苗系统中生物滤池对水质的净化作用%Water purification effect of biofilter in Macrobrachium rosenbergii seeds nursery system

    Institute of Scientific and Technical Information of China (English)

    辛建美; 李倩; 周志明; 胡廷尖; 王军毅; 刘士力; 王雨辰

    2014-01-01

    Water purification effect of biological membranes in circulating water nursery system of Macrobrachium rosenbergii was studied. The results showed that water pollutant clearance rates of biological membranes were higher in the early seedling stage and the highest clearance rates for total nitrogen (TN), total phosphorus (TP), ammonia nitrogen (NH3-N), nitrite nitrogen (NO2-N) and CODMn were up to 46.13%, 58.33%, 22.68%, 32.22% and 7.73% respectively. In the middle stage, clearance rates for each contaminant had different degrees of decline. Changing the water of the seedling pool during middle seedling stage effectively controlled the concentration of pollutants in water.%研究了生物膜对罗氏沼虾( Macrobrachium rosenbergii)循环水育苗系统水体的净化效果.结果表明,在罗氏沼虾育苗早期,生物膜对水体中污染物的清除率均较高,对水体中总氮( TN)最高清除率可达46.13%,对总磷(TP)最高清除率可达58.33%,对氨氮(NH3-N)最高清除率可达22.68%,对亚硝酸盐氮(NO2-N)最高清除率可达32.22%,对化学需氧量CODMn最高清除率可达7.73%.育苗中期,生物膜对各污染物清除率均有不同程度的减弱.于育苗中期对育苗池进行一次换水可以有效控制水体中污染物的浓度.

  17. Bomba sangüínea espiral: concepção, desenvolvimento e aplicação clínica de projeto original Spiral blood pump: conception, development and clinical application of the original project

    Directory of Open Access Journals (Sweden)

    Jarbas J. Dinkhuysen

    2007-06-01

    Full Text Available INTRODUÇÃO: O trabalho aborda projeto original relativo à concepção, ao desenvolvimento e à aplicação clínica de bomba sangüínea que associa forças centrífuga e axial de propulsão hidráulica, baseada no princípio de Arquimedes, denominada Bomba Espiral (BE, tendo recebido Patente Nacional e Relatório Preliminar Internacional categorizando-a como invento. MÉTODO: Visa avaliar sua capacidade hidrodinâmica e seu impacto aos elementos figurados do sangue por meio de testes "in vitro", como Eficiência Hidrodinâmica, Hemólise Normalizada e Visibilização de Escoamento, e, nos testes "in vivo" experimentais, feitos em carneiros submetidos a Circulação Extracorpórea (CEC, e clínico, em 43 pacientes submetidos a operações cardíacas com CEC, nas quais o elemento propulsor foi a BE. RESULTADOS: Na dependência da distância entre o rotor e a carcaça (fenda da bomba pôde-se observar que com 1,5 mm gerou escoamento ao redor de 9 L/min, pressão acima de 400mmHg com 1500 rotações por minuto (rpm, índices de Hemólise Normalizada não superiores a 0,0375 g/100l em condições de alto fluxo e pressão, e pelo estudo de Visibilização do Escoamento no interior da bomba não se detectou áreas de estagnação ou turbulência na entrada, saída e junto à extremidade dos fusos. Nas pesquisas "in vivo" experimentais em ovinos em CEC por 6 horas a BE foi capaz de manter parâmetros pressóricos adequados e Hemoglobina Livre entre 16,36 mg% e 44,90 mg%. Durante sua aplicação em cirurgias cardíacas com CEC, num grupo de 43 pacientes, pôde-se constatar variações pré e pós-CEC, na Hemoglobina Livre de 9,34 a 44,16 mg%, no Fibrinogênio, de 236,65 a 547,26 mg%, na contagem do número de Plaquetas de 152,465 a 98,139, Desidrogenase Láctica, de 238,12 a 547,26 mg%, com tempo de coagulação ativada ao redor de 800 seg. quando em CEC. CONCLUSÕES: A BE mostrou resolutividade por gerar escoamento e pressão adequados, sem causar

  18. Las masacres del olvido. Napalpí y Rincón Bomba en la genealogía del genocidio y el racismo de estado en la Argentina

    Directory of Open Access Journals (Sweden)

    Héctor Hugo Trinchero

    2009-07-01

    Full Text Available

    El objetivo del artículo es intentar trazar una genealogía de algunas prácticas genocidas en la Argentina y, de esta manera, poder comprender las formas recurrentes de masacres sobre población indígena y no indígena producidas por el Estado nacional argentino en su proceso de formación neo-colonial. Específicamente, se detendrá en dos casos que sucedieron ya bien entrado el siglo pasado, es decir, muy a posteriori de la pretendida solución final del General Roca en el sur y las campañas de exterminio desarrolladas por el ejército argentino en el norte y muy anteriores al genocidio de la última dictadura militar. Se trata de la masacre de Napalpí, llevada a cabo en el año 1924 en la provincia de Chaco, y la masacre denominada "Rincón Bomba", perpetrada en el año 1947 en la provincia de Formosa. Ambas masacres de pueblos originarios están siendo revisadas recientemente como un necesario ejercicio de la verdad y la memoria.

  19. Neurotrophic factor - Characterization and partial purification

    Science.gov (United States)

    Popiela, H.; Ellis, S.

    1981-01-01

    Recent evidence suggests that neurotrophic activity is required for the normal proliferation and development of muscle cells. The present paper reports a study of the purification and characterization of a neurotrophic factor (NTF) from adult chicken ischiatic-peroneal nerves using two independent quantitative in vitro assay systems. The assays were performed by the measurement of the incorporation of tritiated thymidine or the sizes of single-cell clones by chick muscle cells grown in culture. The greatest amount of neutrotrophic activity is found to be extracted at a pH of 8; aqueous suspensions of the activity are stable to long-term storage at room temperature. The specific activity of the substance is doubled upon precipitation with ammonium sulfate or after gel filtration, and increase 4 to 5 fold after salt gradient elution from DEAE cellulose columns. The active fraction obtained after gel filtration and rechromatography on DEAE cellulose exhibits a 7 to 10-fold increase in specific activity. Electrophoresis of the most highly purified material yields a greatly concentrated band at around 80,000 daltons. Although NTF is purified almost 10-fold as indicated by the increase in specific activity, the maximum activity of the partially purified material is greatly reduced, possibly due to a requirement for a cofactor for the expression of maximum activity.

  20. TMI-2 purification demineralizer resin study

    Energy Technology Data Exchange (ETDEWEB)

    Thompson, J D; Osterhoudt, T R

    1984-05-01

    Study of the Makeup and Purification System demineralizers at TMI-2 has established that fuel quantities in the vessels are low, precluding criticality, that the high radioactive cesium concentration on the demineralizer resins can be chemically removed, and that the demineralizer resins can probably be removed from the vessels by sluicing through existing plant piping. Radiation measurements from outside the demineralizers establishing that there is between 1.5 and 5.1 (probably 3.3) lb of fuel in the A vessel and less than that amount in the B vessel. Dose rates up to 2780 R per hour were measured on contact with the A demineralizer. Remote visual observation of the A demineralizer showed a crystalline crust overlaying amber-colored resins. The cesium activity in solid resin samples ranged from 220 to 16,900 ..mu..Ci/g. Based on this information, researchers concluded that the resins cannot be removed through the normal pathway in their present condition. Studies do show that the resins will withstand chemical processing designed to rinse and elute cesium from the resins. The process developed should work on the TMI-2 resins.

  1. Effect of Replacement of New Reverse Osmosis Film of Water Purification System to Clinical Biochemistry%实验室纯水系统更换反渗透膜对生化检验的影响

    Institute of Scientific and Technical Information of China (English)

    莫基浩; 刘鲜茹; 李少侠

    2014-01-01

    目的:研究实验室纯水系统更换新反渗透膜后,所产纯水的质量是否符合生化检验的要求并评价其影响。方法通过Beckman Coulter生化分析系统配套纯水机天创TCHS-RO/100A自带电阻表和 HM COM-100电导率测量笔连续监测新产纯水中的实时离子含量。用正常纯水在 AU680上重复20次测量 ALT,AST,TBIL,ALP,GGT,TP,ALB,BUN, CR,UA,GLU,TC,TG,HDL,LDL,CK,LDH,P,Ca(对照组),更换为新产纯水后进行相同试验操作(试验组),以评估新产纯水对生化分析的影响。连续5天,每天3次(间隔2 h)监测新产纯水的 TG试剂空白,监测水中甘油水平。结果开机30 min后,HM COM-100测得电导率降至(0.1±0.0)μS/cm,机载电阻表测得新产纯水在开机50 min后稳定在18.25 MΩcm。在用 AU680进行生化分析时,对照组中TG测量值=1.04 mmol/L,试验组=21.39 mmol/L,差异有统计学意义(P<0.01),而除TG其他生化项目偏倚均在5%以内,CV<1/4CLIA’88,差异无统计学意义(P>0.05)。TG试剂空白在第5天稳定在A660nm=0.0156±0.0004。结论新纯水系统在生产约2800 L水后,所产纯水中的甘油可以清除干净。实验室纯水系统在更换反渗透膜后,需要对水质进行连续监测,以评价其对常规生化项目检测的影响。%Objective To test if the quality of produced water could come up to the standards of clinical biochemistry,and e-valuate the effect of the produced water after replacement of a new reverse osmosis film of the laboratory water purification system.Methods The ion concentration of the produced water was tested by ohmmeter in Beckman Coulter supporting TianChuang water purification system TCHS-RO/100A and HM COM-100 conductivity meter.The biochemical index ALT,AST,TBIL,ALP,GGT,TP,ALB,BUN,CR,UA,GLU,TC,TG,HDL,LDL,CK,LDH,P,Ca of mixed serum were tested 20 times by AU680 under the condition of

  2. Membrane Purification Cell for Aluminum Recycling

    Energy Technology Data Exchange (ETDEWEB)

    David DeYoung; James Wiswall; Cong Wang

    2011-11-29

    Recycling mixed aluminum scrap usually requires adding primary aluminum to the scrap stream as a diluent to reduce the concentration of non-aluminum constituents used in aluminum alloys. Since primary aluminum production requires approximately 10 times more energy than melting scrap, the bulk of the energy and carbon dioxide emissions for recycling are associated with using primary aluminum as a diluent. Eliminating the need for using primary aluminum as a diluent would dramatically reduce energy requirements, decrease carbon dioxide emissions, and increase scrap utilization in recycling. Electrorefining can be used to extract pure aluminum from mixed scrap. Some example applications include producing primary grade aluminum from specific scrap streams such as consumer packaging and mixed alloy saw chips, and recycling multi-alloy products such as brazing sheet. Electrorefining can also be used to extract valuable alloying elements such as Li from Al-Li mixed scrap. This project was aimed at developing an electrorefining process for purifying aluminum to reduce energy consumption and emissions by 75% compared to conventional technology. An electrolytic molten aluminum purification process, utilizing a horizontal membrane cell anode, was designed, constructed, operated and validated. The electrorefining technology could also be used to produce ultra-high purity aluminum for advanced materials applications. The technical objectives for this project were to: - Validate the membrane cell concept with a lab-scale electrorefining cell; - Determine if previously identified voltage increase issue for chloride electrolytes holds for a fluoride-based electrolyte system; - Assess the probability that voltage change issues can be solved; and - Conduct a market and economic analysis to assess commercial feasibility. The process was tested using three different binary alloy compositions (Al-2.0 wt.% Cu, Al-4.7 wt.% Si, Al-0.6 wt.% Fe) and a brazing sheet scrap composition (Al-2

  3. Cyclodextrin purification with hollow fibers

    Energy Technology Data Exchange (ETDEWEB)

    Berthod, A. (Univ. de Lyon 1, Villeubranne Cedex (France)); Jin, Heng Liang,; Armstrong, D.W. (Univ. of Missouri, Rolla (USA))

    1991-01-01

    Cyclodextrins are cyclic 1-4 linked oligomers of {alpha}-D-glucopyranose prepared from starch hydrolysis through enzymatic reactions. Mixtures of the three main cyclodextrins (CD), {alpha}-, {beta}-, and {gamma}-CDs, are always produced. A possible facile purification process is proposed. Permeation through hollow fibers made of a perfluorinated ionomer membrane. Nafion type, is shown to be an effective way to separate {alpha}-CD from {beta}- and {gamma}-CD. {Alpha}-CD with 95% purity was obtained after permeation through a Nafion hollow fiber of an equimolar 0.02 M solution of the three CDs. The fiber had a 56 cm{sup 2}/cm{sup 3} surface area per volume ratio. Kinetic studies and continuous extraction experiments with a 2-m coiled fiber showed that it is possible to obtain a 11.5 g {alpha}-CD solution with 92.4% purity or a 0.6 g {alpha}-CD solution with 97.2% purity, depending on the flow rate. The transport of CDs through the membrane could be due to moving water pools inside the ionomer. The small {alpha}-CD fits easily in such pools when the large {beta}- and {gamma}-CDs are excluded by steric hindrance. Temperature raises increased the permeation rates while decreasing the selectivity. The process could be scaled-up associating hollow fibers in bundle.

  4. Ionic behavior of treated water at a water purification plant

    OpenAIRE

    Yanagida, Kazumi; Kawahigashi, Tatsuo

    2012-01-01

    [Abstract] Water at each processing stage in a water purification plant was extracted and analyzed to investigate changes of water quality. Investigations of water at each processing stage at the water purification plant are discussed herein.

  5. Cloning, high-level expression, purification and characterization of a ...

    African Journals Online (AJOL)

    Cloning, high-level expression, purification and characterization of a staphylokinase variant, SakøC, ... African Journal of Biotechnology ... Hence in this study, we reported the cloning, high-level expression, purification and characterization of ...

  6. Ionic behavior of treated water at a water purification plant

    OpenAIRE

    Yanagida, Kazumi; Kawahigashi, Tatsuo

    2012-01-01

    [Abstract] Water at each processing stage in a water purification plant was extracted and analyzed to investigate changes of water quality. Investigations of water at each processing stage at the water purification plant are discussed herein.

  7. Isolation, purification and properties of lipase from Pseudomonas ...

    African Journals Online (AJOL)

    Isolation, purification and properties of lipase from Pseudomonas aeruginosa. ... medium agar containing Tween 80 or olive oil as the only source of carbon. ... through the purification procedure of ammonium sulphate precipitation and diethyl ...

  8. Novel peptide ligand with high binding capacity for antibody purification

    DEFF Research Database (Denmark)

    Lund, L. N.; Gustavsson, P. E.; Michael, R.

    2012-01-01

    Small synthetic ligands for protein purification have become increasingly interesting with the growing need for cheap chromatographic materials for protein purification and especially for the purification of monoclonal antibodies (mAbs). Today, Protein A-based chromatographic resins are the most ......-aggregated IgG, indicating that the ligand could be used both as a primary purification step of IgG as well as a subsequent polishing step. (C) 2012 Elsevier B.V. All rights reserved....

  9. Capillary Ion Concentration Polarization for Power-Free Salt Purification

    Science.gov (United States)

    Park, Sungmin; Jung, Yeonsu; Cho, Inhee; Kim, Ho-Young; Kim, Sung Jae

    2014-11-01

    In this presentation, we experimentally and theoretically demonstrated the capillary based ion concentration polarization for power-free salt purification system. Traditional ion concentration polarization phenomenon has been studied for a decade for both fundamental nanoscale fluid dynamics and novel engineering applications such as desalination, preconcentration and energy harvesting devices. While the conventional system utilizes an external power source, the system based on capillary ion concentration polarization is capable of perm-selective ion transportation only by capillarity so that the same ion depletion zone can be formed without any external power sources. An ion concentration profile near the nanostructure was tracked using fluorescent probes and analyzed by solving the modified Nernst-Planck equation. As a result, the concentration in the vicinity of the nanostructure was at least 10 times lower than that of bulk electrolyte and thus, the liquid absorbed into the nanostructure had the low concentration. This mechanism can be used for the power free salt purification system which would be significantly useful in underdeveloped and remote area. This work was supported by Samsung Research Funding Center of Samsung Electronics under Project Number SRFC-MA1301-02.

  10. Expression and purification of a human, soluble Arylsulfatase A for Metachromatic Leukodystrophy enzyme replacement therapy.

    Science.gov (United States)

    Martino, Sabata; Consiglio, Antonella; Cavalieri, Cristina; Tiribuzi, Roberto; Costanzi, Egidia; Severini, Giovanni Maria; Emiliani, Carla; Bordignon, Claudio; Orlacchio, Aldo

    2005-05-25

    The production of active Arylsulfatase A is a key step in the development of enzyme replacement therapy for Metachromatic Leukodystrophy. To obtain large amounts of purified Arylsulfatase A for therapeutic use, we combined a retroviral expression system with a versatile and rapid purification protocol that can easily and reliably be adapted to high-throughput applications. The purification method consists of an initial ion-exchange DEAE-cellulose chromatography step followed by immuno-affinity purification using a polyclonal antibody against a 29-mer peptide of the Arylsulfatase A sequence. Immuno-adsorbed protein was eluted with a combination of acidic pH and an optimal concentration of the 29-mer peptide. This protocol reproducibly yielded approximately 100 microg of >99% pure human Arylsulfatase A, corresponding to 152 mU of enzyme activity, per liter of culture medium with properties similar to those of human non-recombinant protein.

  11. Carbon nanotube membranes with ultrahigh specific adsorption capacity for water desalination and purification.

    Science.gov (United States)

    Yang, Hui Ying; Han, Zhao Jun; Yu, Siu Fung; Pey, Kin Leong; Ostrikov, Kostya; Karnik, Rohit

    2013-01-01

    Development of technologies for water desalination and purification is critical to meet the global challenges of insufficient water supply and inadequate sanitation, especially for point-of-use applications. Conventional desalination methods are energy and operationally intensive, whereas adsorption-based techniques are simple and easy to use for point-of-use water purification, yet their capacity to remove salts is limited. Here we report that plasma-modified ultralong carbon nanotubes exhibit ultrahigh specific adsorption capacity for salt (exceeding 400% by weight) that is two orders of magnitude higher than that found in the current state-of-the-art activated carbon-based water treatment systems. We exploit this adsorption capacity in ultralong carbon nanotube-based membranes that can remove salt, as well as organic and metal contaminants. These ultralong carbon nanotube-based membranes may lead to next-generation rechargeable, point-of-use potable water purification appliances with superior desalination, disinfection and filtration properties.

  12. Two-Step Purification of Cordycepin from Cordyceps Millitaris by High-Speed Countercurrent Chromatography

    Science.gov (United States)

    Ju, Xiuyun; Sun, Yong; Cao, Xiaoying; Jiang, Jihong; Zhang, Tianyou; Ito, Yoichiro

    2009-01-01

    Cordycepin is successfully isolated and purified from Cordyceps millitaris in two-step purification by high-speed countercurrent chromatography. Two solvent systems, ethyl acetate–1-butanol–water (3:2:5, v/v/v) and trichloromethane–methanol–1-butanol–water (2:1:0.25:1, v/v/v/v), were used for the two-step purification. The purity of the prepared cordycepin was 98.1% according to the high-performance liquid chromatography analysis. PMID:20046921

  13. Purification and Characterization of Tryptophan Hydroxylase

    DEFF Research Database (Denmark)

    Haahr, Lærke Tvedebrink

    This thesis deals with the purification and characterization of the iron-containing enzyme tryptophan hydroxylase (TPH). TPH exists in two isoforms, called TPH1 and TPH2. Each isoform consists of threestructural distinct domains: the regulatory, the catalytic and the tetramerization domain. TPH...... of this project was to developpurification methods for full-length TPH1 and TPH2 as well as to characterize purified TPH variants. A successful purification method for full-length human TPH1 (hTPH1) was developed, which resulted in pure, active and stable protein. The method includes affinity-purification using....... The crystallization procedure for the catalytic domain of gallus gallus TPH1 (cgTPH1) was optimized to faster crystal growth by addition of tryptophan and incubation at room temperature. Crystals without imidazole in the crystallization conditions could be obtained. The solved structures were however of poor quality...

  14. Reconsidering Rapid Qubit Purification by Feedback

    CERN Document Server

    Wiseman, H M

    2006-01-01

    This paper reconsiders the properties of a scheme for the rapid purification of the quantum state of a qubit, proposed recently in Jacobs 2003 Phys. Rev. A67 030301(R). The qubit starts in a completely mixed state, and information is obtained by a continuous measurement. Jacobs' rapid purification protocol uses Hamiltonian feedback control to maximise the average purity of the qubit for a given time, with a factor of two increase in the purification rate over the no-feedback protocol. However, by re-examining the latter approach, we show that it mininises the average time taken for a qubit to reach a given purity. In fact, the average time taken for the no-feedback protocol beats that for Jacobs' protocol by a factor of two. We discuss how this is compatible with Jacobs' result, and the usefulness of the different approaches.

  15. Purification of Carbon Nanotubes: Alternative Methods

    Science.gov (United States)

    Files, Bradley; Scott, Carl; Gorelik, Olga; Nikolaev, Pasha; Hulse, Lou; Arepalli, Sivaram

    2000-01-01

    Traditional carbon nanotube purification process involves nitric acid refluxing and cross flow filtration using surfactant TritonX. This is believed to result in damage to nanotubes and surfactant residue on nanotube surface. Alternative purification procedures involving solvent extraction, thermal zone refining and nitric acid refiuxing are used in the current study. The effect of duration and type of solvent to dissolve impurities including fullerenes and P ACs (polyaromatic compounds) are monitored by nuclear magnetic reasonance, high performance liquid chromatography, and thermogravimetric analysis. Thermal zone refining yielded sample areas rich in nanotubes as seen by scanning electric microscopy. Refluxing in boiling nitric acid seem to improve the nanotube content. Different procedural steps are needed to purify samples produced by laser process compared to arc process. These alternative methods of nanotube purification will be presented along with results from supporting analytical techniques.

  16. Purification process for vertically aligned carbon nanofibers

    Science.gov (United States)

    Nguyen, Cattien V.; Delziet, Lance; Matthews, Kristopher; Chen, Bin; Meyyappan, M.

    2003-01-01

    Individual, free-standing, vertically aligned multiwall carbon nanotubes or nanofibers are ideal for sensor and electrode applications. Our plasma-enhanced chemical vapor deposition techniques for producing free-standing and vertically aligned carbon nanofibers use catalyst particles at the tip of the fiber. Here we present a simple purification process for the removal of iron catalyst particles at the tip of vertically aligned carbon nanofibers derived by plasma-enhanced chemical vapor deposition. The first step involves thermal oxidation in air, at temperatures of 200-400 degrees C, resulting in the physical swelling of the iron particles from the formation of iron oxide. Subsequently, the complete removal of the iron oxide particles is achieved with diluted acid (12% HCl). The purification process appears to be very efficient at removing all of the iron catalyst particles. Electron microscopy images and Raman spectroscopy data indicate that the purification process does not damage the graphitic structure of the nanotubes.

  17. 高含硫净化装置胺液系统的运行及优化%Optimization and Operation of Amine Liquid System of High-sulfur Purification Device

    Institute of Scientific and Technical Information of China (English)

    曹生伟; 逯敬一; 曹爱娟; 刘炜; 术阿杰; 张恒伟; 岳森

    2011-01-01

    During operation process of high sulfur purification device, the foaming problem of N-methyl diethanolamine (MDEA) solution becomes more serious; it can easily cause the gas phase to carry over liquid into later pipeline in the device, and even can cause the tower bumping violently under serious condition. Based on review of operational parameters, it is drawn that MDEA foam is mainly caused by differential pressure increase in absorption tower. Combined with site actual operation, the cause of MDEA solution foaming is found to be combination of variety of problems. Technology scheme for MDEA solution system problem of foaming is put forward. By adding anti-foam agent to combined device, cleaning mechanical filter in filtration system, and timely replacing activate carbon in activated carbon filter, adjusting steam inlet temperature of re-boiler, the target of reduction of production fluctuation and amine liquid loss caused by amine foaming, removing impurities in amine liquid, and reduction of amine degradation are achieved, and detailed experimental data are obtained. Data analysis shows that good production result is achieved with the methods.%高含硫净化装置在运行过程中,N-甲基二乙醇胺(MDEA)溶液发泡问题变得尤为突出,该工况容易造成装旨后路气相带液,严重时会引起冲塔。通过观察装置运行参数,判断总结出MDEA发泡的主要依据是吸收塔的压差上升。结合现场实际操作,发现引起MDEA溶液发泡是各种问题多样化的结果,分别给出解决MDEA溶液系统发泡问题的技术方案。通过对联合装置加入阻泡剂、清洗过滤系统机械过滤器、及时更换活性炭过滤器的活性炭、调整重沸器蒸汽入口的温度等,减小了胺液发泡造成的生产波动及胺液的跑损,实现了除去胺液的杂质和减少胺液降解等目标,得出了详尽的实验数据。数据分析表明该方案取得了较好的生产效果,达到了降耗目的。

  18. Expression and affinity purification of recombinant proteins from plants

    Science.gov (United States)

    Desai, Urvee A.; Sur, Gargi; Daunert, Sylvia; Babbitt, Ruth; Li, Qingshun

    2002-01-01

    With recent advances in plant biotechnology, transgenic plants have been targeted as an inexpensive means for the mass production of proteins for biopharmaceutical and industrial uses. However, the current plant purification techniques lack a generally applicable, economic, large-scale strategy. In this study, we demonstrate the purification of a model protein, beta-glucuronidase (GUS), by employing the protein calmodulin (CaM) as an affinity tag. In the proposed system, CaM is fused to GUS. In the presence of calcium, the calmodulin fusion protein binds specifically to a phenothiazine-modified surface of an affinity column. When calcium is removed with a complexing agent, e.g., EDTA, calmodulin undergoes a conformational change allowing the dissociation of the calmodulin-phenothiazine complex and, therefore, permitting the elution of the GUS-CaM fusion protein. The advantages of this approach are the fast, efficient, and economical isolation of the target protein under mild elution conditions, thus preserving the activity of the target protein. Two types of transformation methods were used in this study, namely, the Agrobacterium-mediated system and the viral-vector-mediated transformation system. Copyright 2002 Elsevier Science (USA).

  19. Teaching Molecular Biology to Undergraduate Biology Students: An Illustration of Protein Expression and Purification

    Science.gov (United States)

    Sommer, Cesar Adolfo; Silva, Flavio Henrique; Novo, Maria Teresa Marques

    2004-01-01

    Practical classes on protein expression and purification were given to undergraduate biology students enrolled in the elective course "Introduction to Genetic Engineering." The heterologous expression of the green fluorescent protein (GFP)* of "Aequorea victoria" is an interesting system for didactic purposes because it can be viewed easily during…

  20. TiO2-Based Advanced Oxidation Nanotechnologies For Water Purification And Reuse

    Science.gov (United States)

    TiO2 photocatalysis, one of the UV-based advanced oxidation technologies (AOTs) and nanotechnologies (AONs), has attracted great attention for the development of efficient water treatment and purification systems due to the effectiveness of TiO2 to generate ...

  1. TiO2-Based Advanced Oxidation Nanotechnologies For Water Purification And Reuse

    Science.gov (United States)

    TiO2 photocatalysis, one of the UV-based advanced oxidation technologies (AOTs) and nanotechnologies (AONs), has attracted great attention for the development of efficient water treatment and purification systems due to the effectiveness of TiO2 to generate ...

  2. ¿Qué debemos conocer de los inhibidores de bomba protones, para su uso en las unidades de dolor? What need to know the Proton Pump Inhibitors to use in the chronic pain clinic?

    Directory of Open Access Journals (Sweden)

    J. A. García-García

    2007-10-01

    Full Text Available Los inhibidores de la bomba de protones (IBP son fármacos útiles para el control de la patología asociada con la acidez gástrica, patología con una alta prevalencia dentro de la población general. En las Unidades de dolor tratamos pacientes con pluripatología y polimedicados, entre ellas patologías asociadas con la acidez gástrica. Así como también utilizamos fármacos como AINES, glucocorticoides, bifosfonatos... que pueden reactivar, empeorar la patología ligada a la acidez. El conocimiento de los aspectos farmacológicos de los IBP, tanto farmacocinéticos como farmacodinámicos, es necesario y preciso para poder elegir el más adecuado para nuestros pacientes con pluripatologia y polimedicados evitando las posibles interacciones farmacológicas que podrían afectar al estado de salud de nuestros pacientes.Protón Pump Inhibitors (PPIs have shown their usefulness in the treatment of acid-related disorders, highly prevalent in normal population. Pain units are used to treating patients with a wide range of diseases, including acid related-disorders, who are also being prescribed many different drugs, like NSAIDs, corticosteroids or biphosponates, which may reactívate or even worsen acid-related disorders. Knowledge of the pharmacological aspects of PPIs, both their pharmacokinetics and pharmacodynamics, is needed to properly choose the one which fits most to our multi-pathologic, multi-treated patients, in order to avoid possible pharmacological interactions than could endanger their health condition.

  3. Streamlined protein expression and purification using cleavable self-aggregating tags

    Directory of Open Access Journals (Sweden)

    Zhou Bihong

    2011-06-01

    Full Text Available Abstract Background Recombinant protein expression and purification remains a fundamental issue for biotechnology. Recently we found that two short self-assembling amphipathic peptides 18A (EWLKAFYEKVLEKLKELF and ELK16 (LELELKLKLELELKLK can induce the formation of active protein aggregates in Escherichia coli (E. coli, in which the target proteins retain high enzymatic activities. Here we further explore this finding to develop a novel, facile, matrix-free protein expression and purification approach. Results In this paper, we describe a streamlined protein expression and purification approach by using cleavable self-aggregating tags comprising of one amphipathic peptide (18A or ELK16 and an intein molecule. In such a scheme, a target protein is first expressed as active protein aggregate, separated by simple centrifugation, and then released into solution by intein-mediated cleavage. Three target proteins including lipase A, amadoriase II and β-xylosidase were used to demonstrate the feasibility of this approach. All the target proteins released after cleavage were highly active and pure (over 90% in the case of intein-ELK16 fusions. The yields were in the range of 1.6-10.4 μg/mg wet cell pellet at small laboratory scale, which is comparable with the typical yields from the classical his-tag purification, the IMPACT-CN system (New England Biolabs, Beverly, MA, and the ELP tag purification scheme. Conclusions This tested single step purification is capable of producing proteins with high quantity and purity. It can greatly reduce the cost and time, and thus provides application potentials for both industrial scale up and laboratorial usage.

  4. Purification of mammalian DNA repair protein XRCC1

    Energy Technology Data Exchange (ETDEWEB)

    Chen, I. [Univ. of California, Berkeley, CA (United States)

    1995-11-01

    Malfunctioning DNA repair systems lead to cancer mutations, and cell death. XRCC1 (X-ray Repair Cross Complementing) is a human DNA repair gene that has been found to fully correct the x-ray repair defect in Chinese hamster ovary (CHO) cell mutant EM9. The corresponding protein (XRCC1) encoded by this gene has been linked to a DNA repair pathway known as base excision repair, and affects the activity of DNA ligase III. Previously, an XRCC1 cDNA minigene (consisting of the uninterrupted coding sequence for XRCC1 protein followed by a decahistidine tag) was constructed and cloned into vector pET-16b for the purpose of: (1) overproduction of XRCC1 in both prokaryotic and eukaryotic cells; and (2) to facilitate rapid purification of XRCC1 from these systems. A vector is basically a DNA carrier that allows recombinant protein to be cloned and overexpressed in host cells. In this study, XRCC1 protein was overexpressed in E. coli and purified by immobilized metal affinity chromatography. Currently, the XRCC1 minigene is being inserted into a new vector [pET-26b(+)] in hopes to increase overexpression and improve purification. Once purified XRCC1 can be crystallized for structural studies, or studied in vitro for its biological function.

  5. The Monitoring and Affinity Purification of Proteins Using Dual Tags with Tetracysteine Motifs

    Science.gov (United States)

    Giannone, Richard J.; Liu, Yie; Wang, Yisong

    Identification and characterization of protein-protein interaction networks is essential for the elucidation of biochemical mechanisms and cellular function. Affinity purification in combination with liquid chromatography-tandem mass spectrometry (LC-MS/MS) has emerged as a very powerful tactic for the identification of specific protein-protein interactions. In this chapter, we describe a comprehensive methodology that uses our recently developed dual-tag affinity purification system for the enrichment and identification of mammalian protein complexes. The protocol covers a series of separate but sequentially related techniques focused on the facile monitoring and purification of a dual-tagged protein of interest and its interacting partners via a system built with tetracysteine motifs and various combinations of affinity tags. Using human telomeric repeat binding factor 2 (TRF2) as an example, we demonstrate the power of the system in terms of bait protein recovery after dual-tag affinity purification, detection of bait protein subcellular localization and expression, and successful identification of known and potentially novel TRF2 interacting proteins. Although the protocol described here has been optimized for the identification and characterization of TRF2-associated proteins, it is, in principle, applicable to the study of any other mammalian protein complexes that may be of interest to the research community.

  6. Purification of His-Tagged Proteins.

    Science.gov (United States)

    Spriestersbach, Anne; Kubicek, Jan; Schäfer, Frank; Block, Helena; Maertens, Barbara

    2015-01-01

    Ni-NTA affinity purification of His-tagged proteins is a bind-wash-elute procedure that can be performed under native or denaturing conditions. Here, protocols for purification of His-tagged proteins under native, as well as under denaturing conditions, are given. The choice whether to purify the target protein under native or denaturing conditions depends on protein location and solubility, the accessibility of the His tag, and the desired downstream application. His-tagged proteins can be purified by a single-step affinity chromatography, namely immobilized metal ion affinity chromatography (IMAC), which is commercially available in different kinds of formats, Ni-NTA matrices being the most widely used. The provided protocols describe protein purification in the batch binding mode and apply gravity-assisted flow in disposable columns; this procedure is simple to conduct and extremely robust. IMAC purification can equally be performed in prepacked columns using FPLC or other liquid chromatography instrumentation, or using magnetic bead-based methods (Block et al., 2009).

  7. Simple and Economic Purification Method of Berberine

    OpenAIRE

    SHU-HUA, LU; Xu, Li; GUI-BAO, LU; TOYOKAZU, KISHI; Setsuko, SEKITA; Motoyoshi, SATAKE; Tianjin Institute for Drug Control; Japan International Corporation Agency; National Institute of Health Sciences

    1998-01-01

    The authers examined for the purification and quantitative analysis of berberine, thin layer chromatography, centrifugal round plate thin layer chromatography, recrystallization, dry silica gel column chromatography, wet silica gel column chromatography, solvent partition and preparative thin layer chromatography. We established a simple and economic method which is a combination of solvent partition and preparative thin layer chromatography.

  8. Expression and Purification of Sperm Whale Myoglobin

    Science.gov (United States)

    Miller, Stephen; Indivero, Virginia; Burkhard, Caroline

    2010-01-01

    We present a multiweek laboratory exercise that exposes students to the fundamental techniques of bacterial expression and protein purification through the preparation of sperm whale myoglobin. Myoglobin, a robust oxygen-binding protein, contains a single heme that gives the protein a reddish color, making it an ideal subject for the teaching…

  9. Expression and Purification of Sperm Whale Myoglobin

    Science.gov (United States)

    Miller, Stephen; Indivero, Virginia; Burkhard, Caroline

    2010-01-01

    We present a multiweek laboratory exercise that exposes students to the fundamental techniques of bacterial expression and protein purification through the preparation of sperm whale myoglobin. Myoglobin, a robust oxygen-binding protein, contains a single heme that gives the protein a reddish color, making it an ideal subject for the teaching…

  10. Purification on N and P in Aquiculturie Wastewater with a Bacteria-Alga System%菌-藻体系去除水产养殖废水中氮和磷的净化实验

    Institute of Scientific and Technical Information of China (English)

    周武旋; 赵江萍; 栗越妍

    2012-01-01

    estimate the purification effect of bacteria-algae system by removal of N and P in the waste water aquaculture. The results show that the bacteria-algae system composed from bacillus,denitrifying bacteria,crescent algae and four tail gate of algae can effectively remove the nitrogen and phosphorus pollutants in the breeding water through their metabolism process forming the original symbiotic relationship. This bacteria-algae system reveals the best reaction time for removing ammonia nitrogen at 24 h and the biggest removal rate of 98%. In the initial density of 5. 0 × 105 cells/ml, the best bacteria-algae volume ratio is 1 : 2 : 2 : 3,namely the best algae bacteria-initial density is 2. 5 × 10s cell/ml,5. 0 × 10s cell/ml, 5. 0 × 105 cell/ml, 10. 0 × 105 cell/ml for the 4 compositions, respectively. The best reaction time for the bacteria-algae soluble system to remove phosphate is at 168 h with removal rate of 100%. In the initial density of 5. 0 × 105 cells/ml,the best volume ratio of bacteria and algae is 1 : 1 : 3 : 2,namely the best algae bacteria-initial density is 2. 5 × 105cell/ml,2. 5×105cell/ml,10. 0 × 105cell/ml,5. 0 × 10scell/ ml, respectively.

  11. A High-Yield Co-Expression System for the Purification of an Intact Drs2p-Cdc50p Lipid Flippase Complex, Critically Dependent on and Stabilized by Phosphatidylinositol-4-Phosphate

    Science.gov (United States)

    Azouaoui, Hassina; Montigny, Cédric; Ash, Miriam-Rose; Fijalkowski, Frank; Jacquot, Aurore; Grønberg, Christina; López-Marqués, Rosa L.; Palmgren, Michael G.; Garrigos, Manuel; le Maire, Marc; Decottignies, Paulette; Gourdon, Pontus; Nissen, Poul; Champeil, Philippe; Lenoir, Guillaume

    2014-01-01

    P-type ATPases from the P4 subfamily (P4-ATPases) are energy-dependent transporters, which are thought to establish lipid asymmetry in eukaryotic cell membranes. Together with their Cdc50 accessory subunits, P4-ATPases couple ATP hydrolysis to lipid transport from the exoplasmic to the cytoplasmic leaflet of plasma membranes, late Golgi membranes, and endosomes. To gain insights into the structure and function of these important membrane pumps, robust protocols for expression and purification are required. In this report, we present a procedure for high-yield co-expression of a yeast flippase, the Drs2p-Cdc50p complex. After recovery of yeast membranes expressing both proteins, efficient purification was achieved in a single step by affinity chromatography on streptavidin beads, yielding ∼1–2 mg purified Drs2p-Cdc50p complex per liter of culture. Importantly, the procedure enabled us to recover a fraction that mainly contained a 1∶1 complex, which was assessed by size-exclusion chromatography and mass spectrometry. The functional properties of the purified complex were examined, including the dependence of its catalytic cycle on specific lipids. The dephosphorylation rate was stimulated in the simultaneous presence of the transported substrate, phosphatidylserine (PS), and the regulatory lipid phosphatidylinositol-4-phosphate (PI4P), a phosphoinositide that plays critical roles in membrane trafficking events from the trans-Golgi network (TGN). Likewise, overall ATP hydrolysis by the complex was critically dependent on the simultaneous presence of PI4P and PS. We also identified a prominent role for PI4P in stabilization of the Drs2p-Cdc50p complex towards temperature- or C12E8-induced irreversible inactivation. These results indicate that the Drs2p-Cdc50p complex remains functional after affinity purification and that PI4P as a cofactor tightly controls its stability and catalytic activity. This work offers appealing perspectives for detailed structural and

  12. Cellulose affinity purification of fusion proteins tagged with fungal family 1 cellulose-binding domain.

    Science.gov (United States)

    Sugimoto, Naohisa; Igarashi, Kiyohiko; Samejima, Masahiro

    2012-04-01

    N- or C-terminal fusions of red-fluorescent protein (RFP) with various fungal cellulose-binding domains (CBDs) belonging to carbohydrate binding module (CBM) family 1 were expressed in a Pichia pastoris expression system, and the resulting fusion proteins were used to examine the feasibility of large-scale affinity purification of CBD-tagged proteins on cellulose columns. We found that RFP fused with CBD from Trichoderma reesei CBHI (CBD(Tr)(CBHI)) was expressed at up to 1.2g/l in the culture filtrate, which could be directly injected into the cellulose column. The fusion protein was tightly adsorbed on the cellulose column in the presence of a sufficient amount of ammonium sulfate and was efficiently eluted with pure water. Bovine serum albumin (BSA) was not captured under these conditions, whereas both BSA and the fusion protein were adsorbed on a phenyl column, indicating that the cellulose column can be used for the purification of not only hydrophilic proteins but also for hydrophobic proteins. Recovery of various fusion proteins exceeded 80%. Our results indicate that protein purification by expression of a target protein as a fusion with a fungal family 1 CBD tag in a yeast expression system, followed by affinity purification on a cellulose column, is simple, effective and easily scalable.

  13. Simple optimization method for partitioning purification of hydrogen networks

    Directory of Open Access Journals (Sweden)

    W.M. Shehata

    2015-03-01

    Full Text Available The Egyptian petroleum fuel market is increasing rapidly nowadays. These fuels must be in the standard specifications of the Egyptian General Petroleum Corporation (EGPC, which required lower sulfur gasoline and diesel fuels. So the fuels must be deep hydrotreated which resulted in increasing hydrogen (H2 consumption for deeper hydrotreating. Along with increased H2 consumption for deeper hydrotreating, additional H2 is needed for processing heavier and higher sulfur crude slates especially in hydrocracking process, in addition to hydrotreating unit, isomerization units and lubricant plants. Purification technology is used to increase the amount of recycled hydrogen. If the amount of recycled hydrogen is increased, the amount of hydrogen that is sent to the furnaces with the off gas will decrease. In this work, El Halwagi et al. (2003 and El Halwagi (2012 optimization methods which are used for recycle/reuse integration systems have been extended to be used in the partitioning purification of hydrogen networks to minimize the hydrogen consumption and the hydrogen discharge. An actual case study and two case studies from the literature are solved to illustrate the proposed method.

  14. 空分设备分子筛纯化系统出口空气中二氧化碳含量超标分析与处理%Analysis and treatment of exceedance of carbon dioxide content in outlet air of molecular sieve purification system of

    Institute of Scientific and Technical Information of China (English)

    张振友

    2011-01-01

    分子筛纯化系统出口空气中二氧化碳含量超标是影响空分设备稳定运行的主要因素之一。文章从分子筛纯化系统加工气体质量,设计制造,安装操作以及分子筛本身性能4方面,综合分析了导致二氧化碳含量超标的根本原因和处理措施。%The exceedance of carbon dioxide content in outlet air of molecular sieve purification system in one of main factors impairing the stable run of air separation plant. Here, the essential causes for the said exceedance of carbon dioxide content are compreh

  15. Purification non-aqueous solution of quantum dots CdSe- CdS-ZnS from excess organic substance-stabilizer by use PE- HD membrane

    Science.gov (United States)

    Kosolapova, K.; Al-Alwani, A.; Gorbachev, I.; Glukhovskoy, E.

    2015-11-01

    Recently, a new simple method for the purification of CdSe-CdS-ZnS quantum dots by using membrane filtration, the filtration process, successfully separated the oleic acid from quantum dots through membranes purification after synthesis; purification of quantum dots is a very significant part of post synthetical treatment that determines the properties of the material. We explore the possibilities of the Langmuir-Blodgett technique to make such layers, using quantum dots as a model system. The Langmuir monolayer of quantum dots were then investigated the surface pressure-area isotherm. From isotherm, we found the surface pressure monolayer changed with time.

  16. Purification of highly chlorinated dioxins degrading enzyme

    Energy Technology Data Exchange (ETDEWEB)

    Ishii, K.; Furuichi, T.; Koike, K.; Kuboshima, M. [Hokkaido Univ. (Japan). Division of Environment Resource Engineering, Graduate School of Engineering

    2004-09-15

    Soil contamination caused by dioxins in and around sites of incinerators for municipal solid waste (MSW) is a concern in Japan. For example, scattering wastewater from a wet gas scrubber at an MSW incinerator facility in Nose, Osaka caused soil and surface water contamination. The concentration of dioxins in the soil was about 8,000 pg-TEQ/g. Other contamination sites include soils on which fly ash has been placed directly or improperly stored and landfill sites that have received bottom and fly ash over a long period. Some countermeasures are required immediately at these dioxins-contaminated sites. We have previously developed bioreactor systems for dioxin-contaminated water and soil. We have shown that a fungus, Pseudallescheria boydii (P. boydii), isolated from activated sludge treating wastewater that contained dioxins, has the ability to degrade highly chlorinated dioxins. A reaction product of octachlorinated dibenzo-p-dioxin (OCDD) was identified as heptachlorinated dibenzo-p-dioxin. Therefore, one of the pathways for degradation of OCDD by this fungus was predicted to be as follows: OCDD is transformed by dechlorination and then one of the remaining aromatic rings is oxidized. To apply P. boydii to on-site technologies (e.g., bioreactor systems), as well as in situ technologies, enzyme treatment using a dioxin-degrading enzyme from P. boydii needs to be developed because P. boydii is a weak pathogenic fungus, known to cause opportunistic infection. As a result, we have studied enzyme purification of nonchlorinated dioxin, namely, dibenzo-pdioxin (DD). However, we did not try to identify enzymes capable of degrading highly chlorinated dioxins. This study has elucidated a method of enzyme assay for measuring OCDD-degrading activity, and has attempted to purify OCDD-degrading enzymes from P. boydii using enzyme assay. In addition, as first step toward purifying 2,3,7,8-tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD), 2,3,7,8-TCDD degradation tests were carried out

  17. Isolation and Purification of Antibiotic Material from Physarum gyrosum

    Science.gov (United States)

    Schroeder, H. R.; Mallette, M. F.

    1973-01-01

    The myxomycete Physarum gyrosum was cultured in its plasmodial stage on agar plates containing 0.025 M phosphate buffer at pH 6.5, 2% bakers' yeast, and 0.2% glucose and was supplemented with live Escherichia coli. Extracts of these plasmodia contained several antibiotic substances. Antibiotic materials were partially purified by dialysis of the agar medium-mold mixture, evaporation of the dialyzate, and butanol extraction of the residue. Further purification in two paper and two thin-layer chromatographic systems gave one product which was pure in six thin-layer chromatographic systems. Antibiotic activity against some gram-positive and gram-negative bacteria and yeasts was demonstrated with partially purified extracts and a paper-chromatographically separated fraction. One pure antibiotic was effective against Bacillus cereus. PMID:4799591

  18. Matrix product purifications for canonical ensembles and quantum number distributions

    Science.gov (United States)

    Barthel, Thomas

    2016-09-01

    Matrix product purifications (MPPs) are a very efficient tool for the simulation of strongly correlated quantum many-body systems at finite temperatures. When a system features symmetries, these can be used to reduce computation costs substantially. It is straightforward to compute an MPP of a grand-canonical ensemble, also when symmetries are exploited. This paper provides and demonstrates methods for the efficient computation of MPPs of canonical ensembles under utilization of symmetries. Furthermore, we present a scheme for the evaluation of global quantum number distributions using matrix product density operators (MPDOs). We provide exact matrix product representations for canonical infinite-temperature states, and discuss how they can be constructed alternatively by applying matrix product operators to vacuum-type states or by using entangler Hamiltonians. A demonstration of the techniques for Heisenberg spin-1 /2 chains explains why the difference in the energy densities of canonical and grand-canonical ensembles decays as 1 /L .

  19. Simultaneous clarification of Escherichia coli culture and purification of extracellularly produced penicillin G acylase using tangential flow filtration and anion-exchange membrane chromatography (TFF-AEMC).

    Science.gov (United States)

    Orr, Valerie; Scharer, Jeno; Moo-Young, Murray; Honeyman, C Howie; Fenner, Drew; Crossley, Lisa; Suen, Shing-Yi; Chou, C Perry

    2012-07-01

    Downstream purification often represents the most cost-intensive step in the manufacturing of recombinant proteins since conventional purification processes are lengthy, technically complicated, and time-consuming. To address this issue, herein we demonstrated the simultaneous clarification and purification of the extracellularly produced recombinant protein by Escherichia coli using an integrated system of tangential flow filtration and anion exchange membrane chromatography (TFF-AEMC). After cultivation in a bench-top bioreactor with 1L working volume using the developed host/vector system for high-level expression and effective secretion of recombinant penicillin G acylase (PAC), the whole culture broth was applied directly to the established system. One-step purification of recombinant PAC was achieved based on the dual nature of membrane chromatography (i.e. microfiltration-sized pores and anion-exchange chemistry) and cross-flow operations. Most contaminant proteins in the extracellular medium were captured by the anion-exchange membrane and cells remained in the retentate, whereas extracellular PAC was purified and collected in the filtrate. The batch time for both cultivation and purification was less than 24h and recombinant PAC with high purity (19 U/mg), yield (72% recovery), and productivity (41 mg of purified PAC per liter of culture) was obtained. Due to the nature of the non-selective protein secretion system and the versatility of ion-exchange membrane chromatography, the developed system can be widely applied for effective production and purification of recombinant proteins.

  20. Purification of crude biodiesel using dry washing and membrane technologies

    Directory of Open Access Journals (Sweden)

    I.M. Atadashi

    2015-12-01

    Full Text Available Purification of crude biodiesel is mandatory for the fuel to meet the strict international standard specifications for biodiesel. Therefore, this paper carefully analyzed recently published literatures which deal with the purification of biodiesel. As such, dry washing technologies and the most recent membrane biodiesel purification process have been thoroughly examined. Although purification of biodiesel using dry washing process involving magnesol and ion exchange resins provides high-quality biodiesel fuel, considerable amount of spent absorbents is recorded, besides the skeletal knowledge on its operating process. Further, recent findings have shown that biodiesel purification using membrane technique could offer high-quality biodiesel fuel with less wastewater discharges. Thus, both researchers and industries are expected to benefit from the development of membrane technique in purifying crude biodiesel. As well biodiesel purification via membranes has been shown to be environmentally friendly. For these reasons, it is important to explore and exploit membrane technology to purify crude biodiesel.

  1. Affinity-based methodologies and ligands for antibody purification: advances and perspectives.

    Science.gov (United States)

    Roque, Ana C A; Silva, Cláudia S O; Taipa, M Angela

    2007-08-10

    Many successful, recent therapies for life-threatening diseases such as cancer and rheumatoid arthritis are based on the recognition between native or genetically engineered antibodies and cell-surface receptors. Although naturally produced by the immune system, the need for antibodies with unique specificities and designed for single application, has encouraged the search for novel antibody purification strategies. The availability of these products to the end-consumer is strictly related to manufacture costs, particularly those attributed to downstream processing. Over the last decades, academia and industry have developed different types of interactions and separation techniques for antibody purification, affinity-based strategies being the most common and efficient methodologies. The affinity ligands utilized range from biological to synthetic designed molecules with enhanced resistance and stability. Despite the successes achieved, the purification "paradigm" still moves interests and efforts in the continuous demand for improved separation performances. This review will focus on recent advances and perspectives in antibody purification by affinity interactions using different techniques, with particular emphasis on affinity chromatography.

  2. Phytofilter - environmental friendly solution for purification of surface plate from urbanized territories

    Science.gov (United States)

    Ruchkinova, O.; Shchuckin, I.

    2017-06-01

    Its proved, that phytofilters are environmental friendly solution of problem of purification of surface plate from urbanized territories. Phytofilters answer the nowadays purposes to systems of purification of land drainage. The main problem of it is restrictions, connecter with its use in the conditions of cold temperature. Manufactured a technology and mechanism, which provide a whole-year purification of surface plate and its storage. Experimentally stated optimal makeup of filtering load: peat, zeolite and sand in per cent of volume, which provides defined hydraulic characteristics. Stated sorbate and ion-selective volume of complex filtering load of ordered composition in dynamic conditions. Estimated dependences of exit concentrations of oil products and heavy metals on temperature by filtering through complex filtering load of ordered composition. Defined effectiveness of purification at phytofiltering installation. Fixed an influence of embryophytes on process of phytogeneration and capacity of filtering load. Recommended swamp iris, mace reed and reed grass. Manufactured phytofilter calculation methodology. Calculated economic effect from use of phytofiltration technology in comparison with traditional block-modular installations.

  3. Porous graphene-based membranes for water purification from metal ions at low differential pressures.

    Science.gov (United States)

    Park, Jaewoo; Bazylewski, Paul; Fanchini, Giovanni

    2016-05-14

    A new generation of membranes for water purification based on weakly oxidized and nanoporous few-layer graphene is here introduced. These membranes dramatically decrease the high energy requirements of water purification by reverse osmosis. They combine the advantages of porous and non-oxidized single-layer graphene, offering energy-efficient water filtration at relatively low differential pressures, and highly oxidized graphene oxide, exhibiting high performance in terms of impurity adsorption. In the reported fabrication process, leaks between juxtaposed few-layer graphene flakes are sealed by thermally annealed colloidal silica, in a treatment that precedes the opening of (sub)nanometre-size pores in graphene. This process, explored for the first time in this work, results in nanoporous graphene flakes that are water-tight at the edges without occluding the (sub)nanopores. With this method, removal of impurities from water occurs through a combination of size-based pore rejection and pore-edge adsorption. Thinness of graphene flakes allows these membranes to achieve water purification from metal ions in concentrations of few parts-per-million at differential pressures as low as 30 kPa, outperforming existing graphene or graphene oxide purification systems with comparable flow rates.

  4. Nanotechnology for water treatment and purification

    CERN Document Server

    Apblett, Allen

    2014-01-01

    This book describes the latest progress in the application of nanotechnology for water treatment and purification. Leaders in the field present both the fundamental science and a comprehensive overview of the diverse range of tools and technologies that have been developed in this critical area. Expert chapters present the unique physicochemical and surface properties of nanoparticles and the advantages that these provide for engineering applications that ensure a supply of safe drinking water for our growing population. Application areas include generating fresh water from seawater, preventing contamination of the environment, and creating effective and efficient methods for remediation of polluted waters. The chapter authors are leading world-wide experts in the field with either academic or industrial experience, ensuring that this comprehensive volume presents the state-of-the-art in the integration of nanotechnology with water treatment and purification. Covers both wastewater and drinking water treatmen...

  5. Purification of nanoparticles by hollow fiber diafiltration

    Science.gov (United States)

    Veeken, J.

    2012-09-01

    Hollow Fiber Diafiltration (Hollow Fiber Tangential Flow Filtration) is an efficient and rapid alternative to traditional methods of nanoparticle purification such as ultracentrifugation, stirred cell filtration, dialysis or chromatography. Hollow Fiber Diafiltration can be used to purify a wide range of nanoparticles including liposomes, colloids, magnetic particles and nanotubes. Hollow Fiber Diafiltration is a membrane based method where pore size determines the retention or transmission of solution components. It is a flow process where the sample is gently circulated through a tubular membrane. With controlled replacement of the permeate or (dialysate), pure nanoparticles can be attained. Hollow Fiber Diafiltration can be directly scaled up from R&D volumes to production. By adding more membrane fibers and maintaining the operating parameters, large volumes can be processed in the same time with the same pressure, and flow dynamics as bench-scale volumes. Keywords: hollow fiber, Diafiltration, filtration, purification, tangential flow filtration.

  6. Purification of Carbon Nanotubes by Proton Irradiation

    Science.gov (United States)

    Kim, Euikwoun; Lee, Jeonggil; Lee, Younman; Jeon, Jaekyun; Kim, Jae-Yong; Kim, Jeongha; Shin, Kwanwoo; Youn, Sang-Pil; Kim, Kyeryung

    2007-10-01

    Carbon nanotubes (CNTs) exhibit variety of superior physical properties including well-defined nanodimensional structure, high electrical and thermal conductivity, and good mechanical stability against external irradiations. Further, a large specific surface area per unit weight suggests that carbon nanotubes could be excellent candidates for gas storage, purification, and separation. However, the practical application of CNTs is limited mainly due to the metallic impurities that were used as a catalyst during the fabrication process. Here, we irradiated CNTs by using high energy proton beams (35.7 MeV at the Bragg Peak). Interestingly, metallic impurities such as Fe, Ni, Co and chunk of amorphous carbon that were attached on the surface of CNTs were completely removed after the irradiation. The mechanism of such the purification process is not understood. The possible speculation will be demonstrated combined with the changes of physical properties including the appearance of the magnetism after the irradiation.

  7. The viability of photocatalysis for air purification.

    Science.gov (United States)

    Hay, Stephen O; Obee, Timothy; Luo, Zhu; Jiang, Ting; Meng, Yongtao; He, Junkai; Murphy, Steven C; Suib, Steven

    2015-01-14

    Photocatalytic oxidation (PCO) air purification technology is reviewed based on the decades of research conducted by the United Technologies Research Center (UTRC) and their external colleagues. UTRC conducted basic research on the reaction rates of various volatile organic compounds (VOCs). The knowledge gained allowed validation of 1D and 3D prototype reactor models that guided further purifier development. Colleagues worldwide validated purifier prototypes in simulated realistic indoor environments. Prototype products were deployed in office environments both in the United States and France. As a result of these validation studies, it was discovered that both catalyst lifetime and byproduct formation are barriers to implementing this technology. Research is ongoing at the University of Connecticut that is applicable to extending catalyst lifetime, increasing catalyst efficiency and extending activation wavelength from the ultraviolet to the visible wavelengths. It is critical that catalyst lifetime is extended to realize cost effective implementation of PCO air purification.

  8. The Viability of Photocatalysis for Air Purification

    Directory of Open Access Journals (Sweden)

    Stephen O. Hay

    2015-01-01

    Full Text Available Photocatalytic oxidation (PCO air purification technology is reviewed based on the decades of research conducted by the United Technologies Research Center (UTRC and their external colleagues. UTRC conducted basic research on the reaction rates of various volatile organic compounds (VOCs. The knowledge gained allowed validation of 1D and 3D prototype reactor models that guided further purifier development. Colleagues worldwide validated purifier prototypes in simulated realistic indoor environments. Prototype products were deployed in office environments both in the United States and France. As a result of these validation studies, it was discovered that both catalyst lifetime and byproduct formation are barriers to implementing this technology. Research is ongoing at the University of Connecticut that is applicable to extending catalyst lifetime, increasing catalyst efficiency and extending activation wavelength from the ultraviolet to the visible wavelengths. It is critical that catalyst lifetime is extended to realize cost effective implementation of PCO air purification.

  9. Purification of Logic-Qubit Entanglement.

    Science.gov (United States)

    Zhou, Lan; Sheng, Yu-Bo

    2016-07-05

    Recently, the logic-qubit entanglement shows its potential application in future quantum communication and quantum network. However, the entanglement will suffer from the noise and decoherence. In this paper, we will investigate the first entanglement purification protocol for logic-qubit entanglement. We show that both the bit-flip error and phase-flip error in logic-qubit entanglement can be well purified. Moreover, the bit-flip error in physical-qubit entanglement can be completely corrected. The phase-flip in physical-qubit entanglement error equals to the bit-flip error in logic-qubit entanglement, which can also be purified. This entanglement purification protocol may provide some potential applications in future quantum communication and quantum network.

  10. Highly efficient recombinant production and purification of streptococcal cysteine protease streptopain with increased enzymatic activity.

    Science.gov (United States)

    Lane, Michael D; Seelig, Burckhard

    2016-05-01

    Streptococcus pyogenes produces the cysteine protease streptopain (SpeB) as a critical virulence factor for pathogenesis. Despite having first been described seventy years ago, this protease still holds mysteries which are being investigated today. Streptopain can cleave a wide range of human proteins, including immunoglobulins, the complement activation system, chemokines, and structural proteins. Due to the broad activity of streptopain, it has been challenging to elucidate the functional results of its action and precise mechanisms for its contribution to S. pyogenes pathogenesis. To better study streptopain, several expression and purification schemes have been developed. These methods originally involved isolation from S. pyogenes culture but were more recently expanded to include recombinant Escherichia coli expression systems. While substantially easier to implement, the latter recombinant approach can prove challenging to reproduce, often resulting in mostly insoluble protein and poor purification yields. After extensive optimization of a wide range of expression and purification conditions, we applied the autoinduction method of protein expression and developed a two-step column purification scheme that reliably produces large amounts of purified soluble and highly active streptopain. This method reproducibly yielded 3 mg of streptopain from 50 mL of expression culture at >95% purity, with an activity of 5306 ± 315 U/mg, and no remaining affinity tags or artifacts from recombinant expression. This improved method therefore enables the facile production of the important virulence factor streptopain at higher yields, with no purification scars that might bias functional studies, and with an 8.1-fold increased enzymatic activity compared to previously described procedures.

  11. Surface processes during purification of InP quantum dots

    OpenAIRE

    2014-01-01

    Recently, a new simple and fast method for the synthesis of InP quantum dots by using phosphine as phosphorous precursor and myristic acid as surface stabilizer was reported. Purification after synthesis is necessary to obtain samples with good optical properties. Two methods of purification were compared and the surface processes which occur during purification were studied. Traditional precipitation with acetone is accompanied by a small increase in photoluminescence. It occurs that during ...

  12. Conductive diamond electrodes for water purification

    Directory of Open Access Journals (Sweden)

    Carlos Alberto Martínez-Huitle

    2007-12-01

    Full Text Available Nowadays, synthetic diamond has been studied for its application in wastewater treatment, electroanalysis, organic synthesis and sensor areas; however, its use in the water disinfection/purification is its most relevant application. The new electrochemistry applications of diamond electrodes open new perspectives for an easy, effective, and chemical free water treatment. This article highlights and summarizes the results of a selection of papers dealing with electrochemical disinfection using synthetic diamond films.

  13. Extracorporeal Blood Purification in Burns: A Review

    Science.gov (United States)

    2014-09-01

    of solute across a semi permeable membrane, CVVH achieves clearance by means of convection (or ‘solute drag’). This is presumed to enhance the...Second International Consensus Conference of the Acute Dialysis Quality Initiative (ADQI) Group. Crit Care 2004;8:R204 12. [54] Haase M, Bellomo R...extended dialysis . Blood Purif 2012;34:246 52. [56] Morgera S, Klonower D, Rocktaschel J, Haase M, Priem F, Ziemer S, et al. TNF elimination with

  14. Purification of tubulin from porcine brain.

    Science.gov (United States)

    Gell, Christopher; Friel, Claire T; Borgonovo, Barbara; Drechsel, David N; Hyman, Anthony A; Howard, Jonathon

    2011-01-01

    Microtubules, polymers of the heterodimeric protein αβ-tubulin, give shape to cells and are the tracks for vesicle transport and chromosome segregation. In vitro assays to study microtubule functions and their regulation by microtubule-associated proteins require the availability of purified αβ-tubulin. In this chapter, we describe the process of purification of heterodimeric αβ-tubulin from porcine brain.

  15. Simplified purification method for Clostridium difficiletoxin A

    Institute of Scientific and Technical Information of China (English)

    Si-Wu Fu; Jing Xue; Ya-Li Zhang; Dian-Yuan Zhou

    2004-01-01

    AIM: To establish the purification method for Clostridiumdifficile ( C. difficile) toxin A.METHODS: C. difficile VPI 10463 filtrate was cultured anaerobically by the dialysis bag methods. And then the toxin A was purified by precipitation with 500 g/L (NH4)2SO4and acid precipitation at pH 5.5, followed by ion-exchange chromatography on DEAE-Toyopearl.RESULTS: Purified toxin A exhibited only one band on nativepolyacrylamide gel electrophoresis (native-PAGE) andOuchterlony double immunodiffusion. The molecular weight of toxin A was estimated to be 550 000. The purified toxin A had a protein concentration of 0.881 mg/mL. The minimum lethal dose was 1×106 MLD/mL (i.p.mice). The cytotoxictiter was 107 CU/mg. The haemagglutinate activity was ata concentration of 1.72 μg/mL. The ratio of fluid volume (mL)accumulated to the length (cm) of the loop was 2.46. CONCLUSION: The modified method for purification of toxin A of C. difficile was simple and convenient. It may be even more suitable for purification of toxin A on large scales.

  16. Purification of scrap aluminum foil and aluminum melt covering and protecting & atomic purification theory

    Institute of Scientific and Technical Information of China (English)

    倪红军; 孙宝德; 刘满平; 丁文江

    2003-01-01

    A new flux, JDN-I, including rare earth compounds, for purification of the scraps of 99.99% aluminum foil was introduced. The experimental results indicate that its function of degassing and deoxidizing is excellent. The hydrogen content of the scrap aluminum foil melt purified by JDN-I flux decreases greatly from 4.5 mL/kg to 1.2 mL/kg at 720 ℃. The tensile strength of the samples refined with JDN-I flux increases by 19.2% and the elongation increases by 38.3% in comparison with those without flux. The purification mechanism of JDN-I was discussed and a theory of covering, protecting & atomic purification was also put forward.

  17. Compressorless Gas Storage and Regenerative Hydrogen Purification Project

    Data.gov (United States)

    National Aeronautics and Space Administration — Microwave regenerative sorption media gas storage/delivery techniques are proposed to address both compressed gas management and hydrogen purification requirements...

  18. Efficient entanglement purification for doubly entangled photon state

    Institute of Scientific and Technical Information of China (English)

    WANG Chuan; SHENG YuBo; LI XiHan; DENG FuGuo; ZHANG Wei; LONG GuiLu

    2009-01-01

    In this paper,we present an efficient purification scheme that improves the efficiency of entanglement purification of the recently proposed entanglement purification scheme for doubly entangled photon states(Phys.Rev.A,2008,77:042315).This modified scheme contains the bit-flip error correction where all the photon pairs can be kept while all the bit-flip errors are corrected and the entanglement purification of phase-flip errors where a wavelength conversion process is used.This scheme has the advantage of high efficiency and a much lower minimum fidelity of the original state.It works under existing technology.

  19. Purification of cytochrome P450 BM-3 as a monooxygenase.

    Science.gov (United States)

    Jun, Huang; Lehe, Mei; Qing, Sheng; Dongqiang, Lin; Shanjing, Yao

    2005-05-01

    After investigating two anion-exchange resins, the purification factor and activity yields of P450 BM-3 were higher with Resource Q than with DEAE-Sepharose FF. Screening of HIC media showed that Source 15ISO was the most suitable for purification of P450 BM-3. An effective isolation and purification procedure of P450 BM-3 was developed and included three steps: 35%-70% saturation (NH(4))(2)SO(4) precipitation, Source 15ISO hydrophobic interaction chromatograph and Sephacryl S-200 gel filtration chromatography. Using this protocol, the purification factor and P450 BM-3 activity recovery was 13.5 and 13.7%, respectively.

  20. Efficient entanglement purification for doubly entangled photon state

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    In this paper,we present an efficient purification scheme that improves the efficiency of entanglement purification of the recently proposed entanglement purification scheme for doubly entangled photon states (Phys.Rev.A,2008,77:042315).This modified scheme contains the bit-flip error correction where all the photon pairs can be kept while all the bit-flip errors are corrected and the entanglement purification of phase-flip errors where a wavelength conversion process is used.This scheme has the advantage of high efficiency and a much lower minimum fidelity of the original state.It works under existing technology.