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Sample records for pure culture studies

  1. Study of cultured bovine capsular bag in pure ocular tissue

    Institute of Scientific and Technical Information of China (English)

    WANG Yan-qing; LI Qiu-ming

    2006-01-01

    @@ The proliferation, differentiation and fibrosis of lens epithelia cells (LECs) is mainly responsible for posterior capsular opacification (PCO). From the primary culture of LECs to the culture of lens capsular bag, the models of posterior capsular opacification have been developed. At present, the most commonly used model is cell culture in medium with serum. But the culture in pure ocular tissue has not been reported. Therefore, we established a new model of posterior capsular opacification-culturing bovine lens capsular bag in pure ocular tissue to exclude the role of serum. Our study established a new culture method to investigate the proliferation,differentiation and apoptosis of lens epithelia cells in the aqueous humor with or without lens cortex and vitreous humor. The purpose of the study is to model posterior capsular opacification in vivo as closely as possible and to discuss the influence of ocular tissue on posterior capsular opacification.

  2. Comparative study of nickel resistance of pure culture and co-culture of Acidithiobacillus thiooxidans and Leptospirillum ferriphilum.

    Science.gov (United States)

    Xu, Ying; Yin, Huaqun; Jiang, Huidan; Liang, Yili; Guo, Xue; Ma, Liyuan; Xiao, Yunhua; Liu, Xueduan

    2013-09-01

    The effect of Ni²⁺ on the growth and functional gene expression of the pure culture and co-culture of Acidithiobacillus thiooxidans and Leptospirillum ferriphilum has been studied. Compared with the pure culture, the co-culture showed a stronger sulfur and ferrous ion oxidation activity. At 100 mM, A. thiooxidans in co-culture grew faster and had 48 h shorter lag phases. The cell number of A. thiooxidans in co-culture was about 5 times higher than that in pure culture. The existence of A. thiooxidans in co-culture activated the expression of some metal resistance genes in L. ferriphilum at least 16 h in advance. A. thiooxidans in co-culture tends to chose more efficient pathways to transport nickel ion, ensuring the export of heavy metal was faster and more effective than that in pure culture. All the data indicated that there were synergetic interactions between iron- and sulfur-oxidizing bacteria under the stress of Ni²⁺.

  3. Bringing Planctomycetes into pure culture

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    Olga Maria Lage

    2012-12-01

    Full Text Available Planctomycetes have been known since the description of Planctomyces bekefii by Gimesi at the beginning of the twentieth century (1924, although the first axenic cultures were only obtained in the 1970s. Since then, eleven genera with fourteen species have been validly named and five candidatus genera belonging to the anaerobic ammonium oxidation, anammox bacteria have also been discovered. However, Planctomycetes diversity is much broader than these numbers indicate, as shown by environmental molecular studies. In recent years the authors have attempted to isolate and cultivate additional strains of Planctomycetes. This paper provides a summary of the isolation work that was carried out to obtain in pure culture Planctomycetes from several environmental sources. The following strains and planctomycetes have been successfully isolated: two freshwater strains from the sediments of an aquarium, which were described as a new genus and species, Aquisphaera giovannonii; several Rhodopirellula strains from the sediments of a water treatment recycling tank of a marine fish farm; and more than 140 planctomycetes from the biofilm community of macroalgae. This collection comprises several novel taxa that are being characterized and described. Improvements in the isolation methodology were made in order to optimize and enlarge the number of Planctomycetes isolated from the macroalgae. The existence of an intimate and an important relationship between planctomycetes and macroalgae reported before by molecular studies is therefore supported by culture dependent methods.

  4. Dark fermentation on biohydrogen production: Pure culture.

    Science.gov (United States)

    Lee, Duu-Jong; Show, Kuan-Yeow; Su, Ay

    2011-09-01

    Biohydrogen is regarded as an attractive future clean energy carrier due to its high energy content and environmental-friendly conversion. While biohydrogen production is still in the early stage of development, there have been a variety of laboratory- and pilot-scale systems developed with promising potential. This work presents a review of literature reports on the pure hydrogen-producers under anaerobic environment. Challenges and perspective of biohydrogen production with pure cultures are also outlined.

  5. Biomineralization of 1,4-dioxane in Pure Culture, Microcosm, and Column Studies Using 13C Labeling

    Science.gov (United States)

    Rolston, H. M.; Azizian, M.; Hyman, M. R.; Semprini, L.

    2016-12-01

    1,4-dioxane (1,4-D), a probable human carcinogen at low (biomineralizes 1,4-D to CO2. Batch experiments have been conducted with pure culture 21198 and in microcosms constructed with aquifer sediments. The rate of resting cell transformation of 1,4-D by ATCC 21198 was over 100 times faster than the rate of CO2 accumulation, indicating the presence of intermediates that were slowly mineralized to CO2 . In microcosms, the use of isobutane as a primary substrate effectively stimulated the native microbial community to transform 1,4-D. Microcosms were also bioaugmented with ATCC 21198. After an initial lag and subsequent additions of isobutane, transformation rates in the native microcosms approached those of the bioaugmented microcosms. Cometabolically active microbes survived several periods of starvation in all microcosms, and nutrient amendment allowed for sustained transformation rates. 13C labeled 1,4-D is currently being used to determine the rates and extents of biomineralization in the microcosms. Column studies are also being conducted to evaluate cometabolism and biominerazation potential of isobutane as a biostimulant and 21198 for bioaugmentation under geochemical and flow conditions more representative of in-situ bioremediation.

  6. A Study of Mercury Methylation Genetics: Qualitative and Quantitative Analysis of hgcAB in Pure Culture

    Science.gov (United States)

    Christensen, G. A.; Wymore, A. M.; King, A. J.; Podar, M.; Hurt, R. A., Jr.; Santillan, E. F. U.; Gilmour, C. C.; Brandt, C. C.; Brown, S. D.; Palumbo, A. V.; Elias, D. A.

    2015-12-01

    Two proteins (HgcA and HgcB) have been determined to be essential for mercury (Hg)-methylation and either one alone is not sufficient for this process. Detection and quantification of these genes to determine at risk environments is critical. Universal degenerate polymerase chain reaction (PCR) primers spanning hgcAB were developed to ascertain organismal diversity and validate that both genes were present as an established prerequisite for Hg-methylation. To confirm this approach, an extensive set of pure cultures with published genomes (including methylators and non-methylators: 13 Deltaproteobacteria, 9 Firmicutes, and 10 methanogenic Archaea) were assayed with the newly designed universal hgcAB primer set. A single band within an agarose gel was observed for the majority of the cultures with known hgcAB and confirmed via Sanger sequencing. For environmental applications, once the potential for Hg-methylation is established from PCR amplification with the universal hgcAB primer set, quantification of clade-specific hgcAB gene abundance is desirable. We developed quantitative polymerase chain reaction (qPCR) degenerate primers targeting hgcA from each of the three dominate clades (Deltaproteobacteria, Firmicutes and methanogenic Archaea) known to be associated with anaerobic Hg-methylation. The qPCR primers amplify virtually all hgcA positive cultures overall and are specific for their designed clade. Finally, to ensure the procedure is robust and sensitive in complex environmental matrices, cells from all clades were mixed in different combinations and ratios to assess qPCR primer specificity. The development and validation of these high fidelity quantitative molecular tools now allows for rapid and accurate risk management assessment in any environment.

  7. Pure Culture Fermentation of Brined Cucumbers.

    Science.gov (United States)

    Etchells, J L; Costilow, R N; Anderson, T E; Bell, T A

    1964-11-01

    The relative abilities of Pediococcus cerevisiae, Lactobacillus plantarum, L. brevis, and several other species of lactic acid bacteria to grow and produce acid in brined cucumbers were evaluated in pure culture fermentations. Such fermentations were made possibly by the use of two techniques, gamma radiation (0.83 to 1.00 Mrad) and hot-water blanching (66 to 80 C for 5 min), designed first to rid the cucumbers of naturally occurring, interfering, and competitive microbial groups prior to brining, followed by inoculation with the desired lactic acid bacteria. Of the nine species tested, strains of the three common to cucumber fermentations, P. cerevisiae, L. plantarum, and L. brevis, grew to the highest populations, and produced the highest levels of brine acidity and the lowest pH values in fermentations at 5.4 to 5.6% NaCl by weight; also, their sequence of active development in fermentations, with the use of a three-species mixture for inoculation, was in the species order just named. This sequence of occurrence was similar to that estimated by others for natural fermentations. The rates of growth and acid production in fermentations with a mixture of P. cerevisiae, L. plantarum, and L. brevis increased as the incubation temperature was increased from 21 to 27 to 32 C; however, the maximal populations and acidities attained were essentially the same for fermentations at each temperature. Further, these same three species were found to be the most salt tolerant of those tested; their upper limit for appreciable growth and measurable acid production was about 8% salt, whereas thermophilic species such as L. thermophilus, L. lactis, L. helveticus, L. fermenti, and L. delbrueckii exhibited a much lower salt tolerance, ranging from about 2.5 to 4.0%. However, certain strains of L. delbrueckii grew very rapidly in cucumbers brined at 2.5 to 3.0% salt, and produced sufficient acid in about 30 hr at 48 C to reduce the brine pH from above 7.0 to below 4.0. An inexpensive

  8. Pure Culture Fermentation of Green Olives1

    Science.gov (United States)

    Etchells, J. L.; Borg, A. F.; Kittel, I. D.; Bell, T. A.; Fleming, H. P.

    1966-01-01

    The method previously developed by us for the pure-culture fermentation of brined cucumbers and other vegetables has been applied successfully to Manzanillo variety olives. Field-run grade fruit was processed first by conventional procedures to remove most of the bitterness. Then the relative abilities of Lactobacillus plantarum, L. brevis, Pediococcus cerevisiae, and Leuconostoc mesenteroides to become established and produce acid in both heat-shocked (74 C for 3 min) and unheated olives, brined at 4.7 to 5.9% NaCl (w/v basis), were evaluated. The heat-shock treatment not only proved effective in ridding the fruit of naturally occurring, interfering, and competitive microbial groups prior to brining and inoculation, but also made the olives highly fermentable with respect to growth and acid production by the introduced culture, particularly L. plantarum. Of the four species used as inocula, L. plantarum was by far the most vigorous in fermentation ability. It consistently produced the highest levels of brine acidity (1.0 to 1.2% calculated as lactic acid) and the lowest pH values (3.8 to 3.9) during the fermentation of heat-shocked olives. Also, L. plantarum completely dominated fermentations when used in two-species (with P. cerevisiae) and three-species (with P. cerevisiae and L. brevis) combinations as inocula. In contrast, when L. plantarum was inoculated into the brines of unheated olives it failed to become properly established; the same was true for the other species tested, but even to a more pronounced degree. L. brevis was the only species used that failed to develop in brines of both heat-shocked and unheated olives. Modification of the curing brine by the addition of lactic acid at the outset, either with or without dextrose, led to a much earlier onset of fermentation with accompanying acid development, as compared to treatments with dextrose alone or nonadditive controls. Reasons for the marked improvement of the fermentability of Manzanillo olives

  9. Isolating highly pure rat spermatogonial stem cells in culture.

    Science.gov (United States)

    Hamra, F Kent; Chapman, Karen M; Wu, Zhuoru; Garbers, David L

    2008-01-01

    Methods are detailed for isolating highly pure populations of spermatogonial stem cells from primary cultures of testis cells prepared from 22- to 24-day-old rats. The procedure is based on the principle that testicular somatic cells bind tightly to plastic and collagen matrices when cultured in serum-containing medium, whereas spermatogonia and spermatocytes do not bind to plastic or collagen when cultured in serum-containing medium. The collagen-non-binding testis cells obtained using these procedures are thus approx. 97% pure spermatogenic cells. Stem spermatogonia are then easily isolated from the purified spermatogenic population during a short incubation step in culture on laminin matrix. The spermatogenic cells that bind to laminin are more than 90% undifferentiated, type A spermatogonia and are greatly enriched in genetically modifiable stem cells that can develop into functional spermatozoa. This method does not require flow cytometry and can also be applied to obtain enriched cultures of mouse spermatogonial stem cells. The isolated spermatogonia provide a highly potent and effective source of stem cells that have been used to initiate in vitro and in vivo culture studies on spermatogenesis.

  10. Pure populations of murine macrophages from cultured embryonic stem cells. Application to studies of chemotaxis and apoptotic cell clearance.

    Science.gov (United States)

    Zhuang, Lihui; Pound, John D; Willems, Jorine J L P; Taylor, A Helen; Forrester, Lesley M; Gregory, Christopher D

    2012-11-30

    Embryonic stem cells provide a potentially convenient source of macrophages in the laboratory. Given the propensity of macrophages for plasticity in phenotype and function, standardised culture and differentiation protocols are required to ensure consistency in population output and activity in functional assays. Here we detail the development of an optimised culture protocol for the production of murine embryonic stem cell-derived macrophages (ESDM). This protocol provides improved yields of ESDM and we demonstrate that the cells are suitable for application to the study of macrophage responses to apoptotic cells. ESDM so produced were of higher purity than commonly used primary macrophage preparations and were functional in chemotaxis assays and in phagocytosis of apoptotic cells. Maturation of ESDM was found to be associated with reduced capacity for directed migration and increased capacity for phagocytic clearance of apoptotic cells. These results show ESDM to be functionally active in sequential phases of interaction with apoptotic cells and establish these macrophage populations as useful models for further study of molecular mechanisms underlying the recognition and removal of apoptotic cells. Copyright © 2012 Elsevier B.V. All rights reserved.

  11. PURE CULTURE METHOD: GIARDIA LAMBLIA FROM DIFFERENT STOOL SAMPLES

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    H.A YOUSEFI

    2000-03-01

    Full Text Available Introduction. Giardiasis is one of the health problems in the world including Iran. To determine the biochemical and biological problems and also identification of various strains, it is essential to obtain pure culture and then mass production of Giardia lamblia. The goal of this study was to isolate this protozoa purely.
    Methods. Giardia lamblia cysts were isolated from 50 stool samples by use of floating of a four - layer of sucrose method. The cysts were transfered to an inducing solution. Subsequently, they were cultured in a modified culture medium (TYIS-33. Following excystation of trophozoite and its multiplication, the parasite was caltured and purified.
    Findings. Excitation of trophozoite was observed in 40 samples (80 percent from which 22 samples (55 percent yielded pure culture. The doubling time was approximately 13hr and the peak of parasite was observed between third and fourth days.
    Conclusion. The proliferation and growth rate of Giardia lamblia have enabled us to use this method widely. Cystein and ascorbic acid which are present in the induction solution, have a key role in excystation of trophozoite. Purification and passage of samples has facilitated the culture of this parasite in vitro. Therefore this method has yielded better results in comparison with other studies. This is probably due to a decrease in the amount of bovine bile or using different strains of Giardia lamblia in the present study.

  12. Pure Land or Pure Mind?: Locus of Awakening and American Popular Religious Culture

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    Richard K. Payne

    2015-03-01

    Full Text Available This essay has two sections, each with its own distinct goal, forming an interrelated whole. The first introduces “locus of awakening,” and applies it to the relative success in America of Zen and Tibetan Buddhisms, compared to Pure Land Buddhism. The explanatory power of the concept is demonstrated by also considering Soka Gakkai. The difference between popular culture treatments of Zen and Tibetan Buddhisms, and Pure Land Buddhism was the problematic leading to identifying locus of awakening as an aspect of Buddhist thought. The second section locates it in the history of Buddhist thought, demonstrating that it is not a modern conceptualization of the path, not one created in response to Euro–American religio-therapeutic culture. Locus of awakening is, instead, part of the continuity of the Buddhist tradition, and does not fall on one side or the other of the sometimes overdrawn dichotomy between Asian and American Buddhisms.

  13. Bioleaching of chalcopyrite by pure and mixed culture

    Institute of Scientific and Technical Information of China (English)

    ZHANG Yan-sheng; QIN Wen-qing; WANG Jun; ZHEN Shi-jie; YANG Cong-ren; ZHANG Jian-wen; NAI Shao-shi; QIU Guan-zhou

    2008-01-01

    The bioleaching of chalcopyrite in shake flasks was investigated by using pure Acidithiobacillus ferrooxidans and mixed culture isolated from the acid mine drainage in Yushui and Dabaoshan Copper Mine in China,marked as YS and DB,respectively.The mixed culture consisted mainly of Acidithiobacillus ferrooxidans,Acidithiobacillus thiooxidans,and Leptospirillum spp.(Leptospirillum ferriphilum and Leptospirillum ferrooxians).The results show that the mixed culture is more efficient than the pure Acidithiobacillus ferrooxidans because of the presence of the sulfur-oxidizing cultures that positively increase the dissolution rate and the recovery of copper from chalcopyrite.The pH value decreases with the decrease of chalcopyrite leaching rate,because of the formation of jarosite as a passivation layer on the mineral surface during bioleaching.In the bioleaching using the mixed culture,low pH is got from the sulfur oxidizing inhibiting,the formation of jarosite.The copper extraction reaches 46.27% in mixed culture and 30.37% in pure Acidithiobacillus ferrooxidans after leaching for 75 d.

  14. FT-IR microspectroscopy in rapid identification of bacteria in pure and mixed culture

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    Fontoura, Inglid; Belo, Ricardo; Sakane, Kumiko; Cardoso, Maria Angélica Gargione; Khouri, Sônia; Uehara, Mituo; Raniero, Leandro; Martin, Airton A.

    2010-02-01

    In recent years FT-IR microspectroscopy has been developed for microbiology analysis and applied successfully in pure cultures of microorganisms to rapidly identify strains of bacteria, yeasts and fungi. The investigation and characterization of microorganism mixed cultures is also of growing importance, especially in hospitals where it is common to poly-microbial infections. In this work, the rapid identification of bacteria in pure and mixed cultures was studied. The bacteria were obtained from the Institute Oswaldo Cruz culture collection at Brazil. Escherichia coli ATCC 10799 and Staphylococcus aureus ATCC 14456 were analyzed, 3 inoculations were examined in triplicate: Escherichia coli, Staphylococcus aureus and a mixed culture of them. The inoculations were prepared according to McFarland 0.5, incubated at 37 ° C for 6 hours, diluted in saline, placed in the CaF2 window and store for one hour at 50°C to obtain thin film. The measurement was performed by Spectrum Spotlight 400 (Perkin-Elmer) equipment in the range of 4000-900 cm-1, with 32 scans using a transmittance technique with point and image modes. The data were processed (baseline, normalization, calculation of first derivate followed by smoothing with 9 point using a Savitzky-Golay algorithm) and a cluster analysis were done by Ward's algorithm and an excellent discrimination between pure and mixed culture was obtained. Our preliminary results indicate that the FT-IR microspectroscopy associated with cluster analysis can be used to discriminate between pure and mixed culture.

  15. The presence of embedded bacterial pure cultures in agar plates stimulate the culturability of soil bacteria

    DEFF Research Database (Denmark)

    Burmølle, Mette; Johnsen, Kaare; Abu Al-Soud, Waleed Mohamad Abdel F

    2009-01-01

    Traditional methods for bacterial cultivation recover only a small fraction of bacteria from all sorts of natural environments, and attempts have been made to improve the bacterial culturability. Here we describe the development of a cultivation method, based on the embedment of pure bacterial cu...

  16. Comparative evaluation of the hydrogen production by mixed consortium, synthetic co-culture and pure culture using distillery effluent.

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    Mishra, Preeti; Roy, Shantonu; Das, Debabrata

    2015-12-01

    Wastewater comprises of various carbon sources. So, the use of microbial consortium may improve the hydrogen production and organic reduction. The present study deals with biohydrogen production by acidogenic mixed consortia (AMC), synthetic co-culture (Klebsiella pneumoniae IIT-BT 08 and Citrobacter freundii IIT-BT L139) and pure culture using distillery effluent (DE). Higher hydrogen yield was observed in case of AMC (9.17 mol/kg CODreduced) as compared to the synthetic co-culture and pure culture. PCR-DGGE analysis indicated that the consortium was predominated by species closely affiliated to Clostridium sp. The average hydrogen production rate was 267 mL/Lh. The maximum hydrogen production rate (Rm), hydrogen production potential (P) and lag time (λ) by AMC using DE were 507.2 mL/Lh, 3729 m/L and 2.04 h, respectively. Maximum gaseous energy recovery by AMC was found to be higher by 21.9% and 45.4% than that of using co-culture and pure culture respectively.

  17. Application of the Mixture Design to Design the Formulation of Pure Cultures in Tibetan kefir

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    To obtain the optimization formulation of pure cultures in Tibetan kefir, the influence of the different mixtures of five strains in the pure cultures in Tibetan kefir on the flavor components in fermented milk was studied using the mixture design. The regression model on microorganism composition and main metabolites was established. The results suggested that the predictable production of lactate reached the maximum of 8.16 g L-1, while the most predictable production of diacetyl, ethanol, and CO2 were 77.23 mg L-1, 4259 mg L-1, and 2.12 g L-1, respectively. Based on these, the response values that satisfied all expectations were optimized, and the most excellent combination was Lactococcus lactis 27%, Leuconostoc mesenteroides 37%, Lactobacillus kefiri 11%, Lactobacillus casei 10%, and Kluyveromyces marxianus 15%. With the aid of analysis software (Design-expert 6.0.5), the formulation of pure cultures in Tibetan kefir can be optimized for several responses and the best formulation can be obtained.

  18. The pure-science ideal and democratic culture.

    Science.gov (United States)

    Daniels, G H

    1967-06-30

    These early experiences of pure scientists will have an unmistakable ring of familiarity to anyone familiar with the current situation. Charles Sanders Peirce, with characteristic insight, had stated the fundamental dilemma of the pure scientist operating within a democratic framework. How can one ask the public to provide support, much less facilities, for the intellectual gratification of one select group? A part of the answer, of course, is simply that one cannot. As long as a group is dependent upon public support it must seek some means of contact with the values of the enveloping society, and the moment it does this it departs in some measure from the ideal purity. The schizophrenic attitude described by Dubos therefore became a professional necessity as soon as the new ideal was adopted. Since the time of Gould, scientists have been able to tell each other that the man who based science's claim to support on grounds of immediate practical utility was "no loyal follower and true friend of science" and, at the same time, to trust that the popularizers and technicians would convey a different message to the public. On the whole, they have not been disappointed in their expectation, and there has been little need for them to go beyond the standard formula : Utility is not to be a test of scientific work, but all knowledge will ultimately prove useful. Since the continued existence of scientists in this society depends upon the believability of that vague claim, there is little likelihood hood that the schizophrenia will disappear.

  19. Fermentative hydrogen production from glucose and starch using pure strains and artificial co-cultures ofClostridium spp.

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    Masset Julien

    2012-05-01

    Full Text Available Abstract Background Pure bacterial strains give better yields when producing H2 than mixed, natural communities. However the main drawback with the pure cultures is the need to perform the fermentations under sterile conditions. Therefore, H2 production using artificial co-cultures, composed of well characterized strains, is one of the directions currently undertaken in the field of biohydrogen research. Results Four pure Clostridium cultures, including C. butyricum CWBI1009, C. pasteurianum DSM525, C. beijerinckii DSM1820 and C. felsineum DSM749, and three different co-cultures composed of (1 C. pasteurianum and C. felsineum, (2 C. butyricum and C. felsineum, (3 C. butyricum and C. pasteurianum, were grown in 20 L batch bioreactors. In the first part of the study a strategy composed of three-culture sequences was developed to determine the optimal pH for H2 production (sequence 1; and the H2-producing potential of each pure strain and co-culture, during glucose (sequence 2 and starch (sequence 3 fermentations at the optimal pH. The best H2 yields were obtained for starch fermentations, and the highest yield of 2.91 mol H2/ mol hexose was reported for C. butyricum. By contrast, the biogas production rates were higher for glucose fermentations and the highest value of 1.5 L biogas/ h was observed for the co-culture (1. In general co-cultures produced H2 at higher rates than the pure Clostridium cultures, without negatively affecting the H2 yields. Interestingly, all the Clostridium strains and co-cultures were shown to utilize lactate (present in a starch-containing medium, and C. beijerinckii was able to re-consume formate producing additional H2. In the second part of the study the co-culture (3 was used to produce H2 during 13 days of glucose fermentation in a sequencing batch reactor (SBR. In addition, the species dynamics, as monitored by qPCR (quantitative real-time PCR, showed a stable coexistence of C. pasteurianum and C

  20. Isolation and Initial Characterization of A Pure Cultures Capable to Degradation Methyl tert- Butyl Ether (MTBE

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    A Nikpey

    2006-07-01

    Full Text Available Methyl tert-butyl ether (MTBE, a gasoline octane enhancer, was introduced as a substitute for lead tetraethyl over 30 years ago. Widespread use of MTBE in gasoline, has introduced MTBE into the environment compartments, mostly into the under ground and surface water and water as a second most frequently detected contaminant. In this study, we have isolated pure cultures from bacterial consortium capable to use MTBE as a sole carbon and energy source. MTBE biodegradation rate was measured in headspace by gas chromatography. Initial liner rates of biodegradation by Pinpoint and white strains were found 2.9 mg and 3 mg MTBE hˉ1 gˉ1 wet biomass, respectively. The results of 16S rDNA PCR disclosed similarities in the banding patterns between the cultures, and the known degrading strain PM1. The results of this study suggest promising perspectives for engineering the in situ bioremediation of MTBE.

  1. Study on pure silica core optical fibers

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    An optimal refractive index profile of pure silica core optical fiber (PSCF) was designed, in combination with the characters of the modified chemical vapor deposition (MCVD) process. Techniques of preform fabrication by a new furnace round heating MCVD process and fiber drawing process were reviewed. Difficulties in doping fluorine in silica, widening the depressed-index cladding and maintaining the index of fiber core were discussed. Methods used to overcome these difficulties were given at the same time. Additionally, the optimal refractive index profiles of PSCF were presented.

  2. A serendipic legacy: Erwin Esmarch's isolation of the first photosynthetic bacterium in pure culture.

    Science.gov (United States)

    Gest, H

    1995-01-01

    During the 1880's, Erwin von Esmarch was a junior associate ('Assistent') of Robert Koch studying bacteria of medical significance. In 1887, he isolated the first example of spiral-shaped bacteria in pure culture, from the dry residue of a dead mouse that he had suspended sometime earlier in Berlin tap-water. Under certain conditions, colonies of the organism were the color of red wine, and this led Esmarch to name the bacterium Spirillum rubrum. Twenty years later, Hans Molisch demonstrated that S. rubrum, an apparent heterotroph, was in fact a non-oxygenic purple photosynthetic bacterium, and it was renamed Rhodospirillum rubrum. Esmarch was a careful investigator and his classic paper of 1887 details the serendipitous isolation and general characteristics of the first pure culture of an anoxyphototroph, which later played a prominent role as an experimental system for study of basic aspects of bacterial photosynthesis. This report includes an English translation of his original paper (in German), a commentary on the historical significance of 'Esmarch's spirillum', and a summary of Esmarch's career.

  3. Production of Fusaric Acid by Fusarium spp. in Pure Culture and in Solid Medium Co-Cultures

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    Nadine Bohni

    2016-03-01

    Full Text Available The ability of fungi isolated from nails of patients suffering from onychomycosis to induce de novo production of bioactive compounds in co-culture was examined. Comparison between the metabolite profiles produced by Sarocladium strictum, by Fusarium oxysporum, and by these two species in co-culture revealed de novo induction of fusaric acid based on HRMS. Structure confirmation of this toxin, using sensitive microflow NMR, required only three 9-cm Petri dishes of fungal culture. A targeted metabolomics study based on UHPLC-HRMS confirmed that the production of fusaric acid was strain-dependent. Furthermore, the detected toxin levels suggested that onychomycosis-associated fungal strains of the F. oxysporum and F. fujikuroi species complexes are much more frequently producing fusaric acid, and in higher amount, than strains of the F. solani species complex. Fusarium strains producing no significant amounts of this compound in pure culture, were shown to de novo produce that compound when grown in co-culture. The role of fusaric acid in fungal virulence and defense is discussed.

  4. Production of Fusaric Acid by Fusarium spp. in Pure Culture and in Solid Medium Co-Cultures.

    Science.gov (United States)

    Bohni, Nadine; Hofstetter, Valérie; Gindro, Katia; Buyck, Bart; Schumpp, Olivier; Bertrand, Samuel; Monod, Michel; Wolfender, Jean-Luc

    2016-03-18

    The ability of fungi isolated from nails of patients suffering from onychomycosis to induce de novo production of bioactive compounds in co-culture was examined. Comparison between the metabolite profiles produced by Sarocladium strictum, by Fusarium oxysporum, and by these two species in co-culture revealed de novo induction of fusaric acid based on HRMS. Structure confirmation of this toxin, using sensitive microflow NMR, required only three 9-cm Petri dishes of fungal culture. A targeted metabolomics study based on UHPLC-HRMS confirmed that the production of fusaric acid was strain-dependent. Furthermore, the detected toxin levels suggested that onychomycosis-associated fungal strains of the F. oxysporum and F. fujikuroi species complexes are much more frequently producing fusaric acid, and in higher amount, than strains of the F. solani species complex. Fusarium strains producing no significant amounts of this compound in pure culture, were shown to de novo produce that compound when grown in co-culture. The role of fusaric acid in fungal virulence and defense is discussed.

  5. Production and optimization of L-glutaminase enzyme from Hypocrea jecorina pure culture.

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    Bülbül, Dilara; Karakuş, Emine

    2013-01-01

    L-Glutaminase (L-glutamine amidohydrolase, EC 3.5.1.2) is the important enzyme that catalyzes the deamination of L-glutamine to L-glutamic acid and ammonium ions. Recently, L-glutaminase has received much attention with respect to its therapeutic and industrial applications. It acts as a potent antileukemic agent and shows flavor-enhancing capacity in the production of fermented foods. Glutaminase activity is widely distributed in plants, animal tissues, and microorganisms, including bacteria, yeasts, and fungi. This study presents microbial production of glutaminase enzyme from Hypocrea jecorina pure culture and determination of optimum conditions and calculation of kinetic parameters of the produced enzyme. The optimum values were determined by using sa Nesslerization reaction for our produced glutaminase enzyme. The optimum pH value was determined as 8.0 and optimum temperature as 50°C for the glutaminase enzyme. The Km and Vmax values, the kinetic parameters, of enzyme produced from Hypocrea jecorina, pure culture were determined as 0.491 mM for Km and 13.86 U/L for Vmax by plotted Lineweaver-Burk graphing, respectively. The glutaminase enzyme from H. jecorina microorganism has very high thermal and storage stability.

  6. The study of antioxidant activity of multicomponent vegetable puree

    Directory of Open Access Journals (Sweden)

    A. V. Trushechkin

    2013-01-01

    Full Text Available Studied the antioxidant activity of multicomponent vegetable puree, consisting of 20 % of eggplant, 20 % of zucchini, 16 % of bell peppers, 15 % of tomatoes, 14 % of carrots, 10 % of onions and 5 % of garlic. These results showed an increase in concentration of the antioxidant activity of puree and, consequently, increase its quality.

  7. Mycotoxins of Aspergillus fumigatus in pure culture and in native bioaerosols from compost facilities.

    Science.gov (United States)

    Fischer, G; Müller, T; Ostrowski, R; Dott, W

    1999-04-01

    Exposure to secondary metabolites of airborne fungi in waste handling facilities is discussed in regard to potential toxic impacts on human health. The relevance of mycotoxins has been intensely studied in connection with contamination of food and feed. Toxic secondary metabolites are expected to be present in airborne spores, but exposure to mycotoxins in bioaerosols has not been studied sufficiently. Aspergillus fumigatus is one of the most frequent species in the air of compost plants. A wide range of secondary metabolites was found in pure cultures of freshly isolated strains of A. fumigatus. Tryptoquivaline, a compound with tremorgenic properties, and trypacidin, for which no toxic properties are described, were found in native bioaerosols in a compost facility. The highly toxic metabolites gliotoxin and verruculogen were not found in the bioaerosols.

  8. Differential sensitivity of aerobic gram-positive and gram-negative microorganisms to 2,4,6-trinitrotoluene (TNT) leads to dissimilar growth and TNT transformation: Results of soil and pure culture studies

    Energy Technology Data Exchange (ETDEWEB)

    Fuller, M.E.; Manning, J.F. Jr.

    1996-07-30

    The effects of 2,4,6-trinitrotoluene (TNT) on indigenous soil populations and pure bacterial cultures were examined. The number of colony-forming units (CFU) appearing when TNT-contaminated soil was spread on 0.3% molasses plates decreased by 50% when the agar was amended with 67 {mu}g TNT mL{sup -1}, whereas a 99% reduction was observed when uncontaminated soil was plated. Furthermore, TNT-contaminated soil harbored a greater number of organisms able to grow on plates amended with greater than 10 {mu}g TNT mL{sup -1}. The percentage of gram-positive isolates was markedly less in TNT-contaminated soil (7%; 2 of 30) than in uncontaminated soil (61%; 20 of 33). Pseudomonas aeruginosa, Pseudomonas corrugate, Pseudomonasfluorescens and Alcaligenes xylosoxidans made up the majority of the gram-negative isolates from TNT-contaminated soil. Gram-positive isolates from both soils demonstrated marked growth inhibition when greater than 8-16 {mu}g TNT mL{sup -1} was present in the culture media. Most pure cultures of known aerobic gram-negative organisms readily degraded TNT and evidenced net consumption of reduced metabolites. However, pure cultures of aerobic gram-positive bacteria were sensitive to relatively low concentrations of TNT as indicated by the 50% reduction in growth and TNT transformation which was observed at approximately 10 {mu}g TNT mL{sup -1}. Most non-sporeforming gram-positive organisms incubated in molasses media amended with 80 {mu}g TNT mL{sup -1} or greater became unculturable, whereas all strains tested remained culturable when incubated in mineral media amended with 98 {mu}g TNT mL{sup -1}, indicating that TNT sensitivity is likely linked to cell growth. These results indicate that gram-negative organisms are most likely responsible for any TNT transformation in contaminated soil, due to their relative insensitivity to high TNT concentrations and their ability to transform TNT.

  9. METHOD FOR INDUCING SPORULATION OF PURE CULTURES OF THE MYXOMYCETE PHYSARUM POLYCEPHALUM

    Science.gov (United States)

    Daniel, John W.; Rusch, Harold P.

    1962-01-01

    Daniel, John W. (University of Wisconsin, Madison) and Harold P. Rusch. Method for inducing sporulation of pure cultures of the myxomycete Physarum polycephalum. J. Bacteriol. 83:234–240. 1962.—Techniques for inducing sporulation of pure cultures of Physarum polycephalum are described. Small plasmodia grown in agitated culture were harvested, allowed to fuse into large plasmodia (1.3 g wet weight each), and, after incubation in the dark on a salts medium, exposed to light for 2 hr. The ensuing formation of sporangia containing spores was complete after 12 to 16 hr. The obligatory conditions for sporulation are: (i) an optimal growth age occurring just prior to the maximal growth of the organism and at a time when nutrients in the medium are exhausted; (ii) 4 days of incubation on a medium containing only inorganic salts and niacin or tryptophan; and (iii) subsequent illumination with light of wavelengths between 350 and 500 mμ. PMID:13883384

  10. Bioelectrochemical reduction of carbon dioxide by pure culture at the cathode

    DEFF Research Database (Denmark)

    Aryal, Nabin; Tremblay, Pier-Luc; Chen, Leifeng

    2014-01-01

    Microbial electrosynthesis (MES) is an innovative approach in which microbes use electricity toreduce carbon dioxide and produce chemical commodities. This process relies on the ability of electroautotrophic microbes to accept electron from an electrode. The concept of MES has already been...... demonstrated with pure cultures of acetogenic bacteria such as Sporomusa ovate DSM-2662 and Clostridium ljungdahlii. Until now, electron transfer rates from the cathode to the bestelectroautotroph, S. ovata, are still orders of magnitude lower than what is observed in bioanodicprocesses with electrigenic...... bacteria. Hence, we are screening other pure cultures for better MES activities. These bacterial species were pre-selected based on several criteria such as their presence in enrichments of environmental samples in MES systems, their capacity to fix CO2, their incapacity to sporulate, and their ability...

  11. Hydrogen isotope fractionation by Methanothermobacter thermoautotrophicus in coculture and pure culture conditions

    Science.gov (United States)

    Yoshioka, Hideyoshi; Sakata, Susumu; Kamagata, Yoichi

    2008-06-01

    We grew a hydrogen-utilizing methanogen, Methanothermobacter thermoautotrophicus strain ΔH, in coculture and pure culture conditions to evaluate the hydrogen isotope fractionation associated with carbonate reduction under low (6 mM; pure culture) concentrations of H 2 in the headspace. In the cocultures, which were grown at 55 °C with a thermophilic butyrate-oxidizing syntroph, the hydrogen isotopic relationship between methane and water was well represented by the following equation: δD=0.725(±0.003)·δDO-275(±3), in which the hydrogen isotope fractionation factor ( αH) was 0.725 ± 0.003. The relationship was consistent with the isotopic data on methane and water from terrestrial fields (a peat bog in Washington State, USA, and a sandy aquifer in Denmark), where carbonate reduction was reported to be the dominant pathway of methanogenesis. In the pure cultures, grown at 55 and 65 °C, the αH values were 0.755 ± 0.014 and 0.749 ± 0.014, respectively. Dependence of αH on growth temperature was not observed. The αH value at 55 °C in the pure culture was slightly higher than that in the coculture, a finding that disagrees with a hypothesis proposed by Burke [Burke, Jr. R. A. (1993) Possible influence of hydrogen concentration on microbial methane stable hydrogen isotopic composition. Chemosphere26, 55-67] that hydrogen isotope fractionation between methane and water increases (and αH decreases) with increasing H 2 concentration.

  12. Development of a PCR test for identification of Haemophilus somnus in pure and mixed cultures

    DEFF Research Database (Denmark)

    Angen, Øystein; Ahrens, Peter; Tegtmeier, Conny

    1998-01-01

    a single colony of H. somnus in the presence of 10(9) CFU of P. multocida even after 2 days of incubation. In conclusion, the present PCR test has been shown to represent a specific test for identification of H. somnus both in pure and mixed cultures. It represents a quick, sensitive and reliable method...... rise to an amplicon in the PCR test. The performance of the test on mixed cultures was evaluated by adding P. multocida to serial dilutions of H. somnus and incubating the agarplates for 1 and 2 days. This showed that the PCR test applied to the harvest from an agarplate can be expected to detect...

  13. Fermentative capabilities and volatile compounds produced by Kloeckera/Hanseniaspora and Saccharomyces yeast strains in pure and mixed cultures during Agave tequilana juice fermentation.

    Science.gov (United States)

    González-Robles, Ivonne Wendolyne; Estarrón-Espinosa, Mirna; Díaz-Montaño, Dulce María

    2015-09-01

    The fermentative and aromatic capabilities of Kloeckera africana/Hanseniaspora vineae K1, K. apiculata/H. uvarum K2, and Saccharomyces cerevisiae S1 and S2 were studied in pure and mixed culture fermentations using Agave tequila juice as the culture medium. In pure and mixed cultures, Kloeckera/Hanseniaspora strains showed limited growth and sugar consumption, as well as low ethanol yield and productivity, compared to S. cerevisiae, which yielded more biomass, ethanol and viable cell concentrations. In pure and mixed cultures, S. cerevisiae presented a similar behaviour reaching high biomass production, completely consuming the sugar, leading to high ethanol production. Furthermore, the presence of S. cerevisiae strains in the mixed cultures promoted the production of higher alcohols, acetaldehyde and ethyl esters, whereas Kloeckera/Hanseniaspora strains stimulated the production of ethyl acetate and 2-phenyl ethyl acetate compounds.

  14. Isotopologue effects during N2O reduction in soils and in pure cultures of denitrifiers

    Science.gov (United States)

    Ostrom, Nathaniel E.; Pitt, Adam; Sutka, Robin; Ostrom, Peggy H.; Grandy, A. Stuart; Huizinga, Kristin M.; Robertson, G. Philip

    2007-06-01

    Site preference (SP), the difference in δ15N between the central and outer nitrogen atoms in N2O, is a powerful approach for apportioning fluxes of N2O from soils to nitrification and denitrification (Sutka et al., 2006). A critical aspect of the use of SP data to apportion sources of N2O to nitrification and denitrification is the need to evaluate data for isotope shifts that may have occurred during N2O reduction in soils prior to its escape to the atmosphere. We present data on the isotopologue effects during reduction of N2O during anaerobic incubation of soils and pure cultures of denitrifying bacteria. Isotopic enrichment factors for N2O reduction in soil mesocosms experiments varied between -9.2 and -1.8‰ for nitrogen and between -25.1 and -5.1‰ for oxygen. In pure cultures of Psuedomonas stutzeri and Psuedomonas denitrificans we observed isotopic enrichment factors for SP of -5.0 and -6.8‰, respectively. We further find that N2O consumption produces consistent relationships between δ18O and δ15N and δ18O and the δ15N of the central N atom in N2O of 2.5 and 1.6, respectively, which are clearly diagnostic of this process. Our results indicate that SP may be altered during reduction of N2O and thus bias evaluations of its origins. To understand the impacts of N2O reduction in soil flux studies on source isotope signals we modeled the isotope effects of N2O production occurring simultaneous with reduction and find increasingly curvilinear relationships between δ18O and δ15N and δ18O and δ15Nα with increased reduction. Consequently, a deviation from the linear mixing relationship between soil-derived and atmospheric N2O is an indication of extensive reduction. On the basis of our characterization of isotopic fractionation during N2O reduction, we show that the rate of reduction would have to be substantially greater than 10% of that of production to impact SP estimates of N2O from denitrification by more than a few percent. Nonetheless, reduction

  15. Pure culture of Metarhizium anisopliae LHL07 reprograms soybean to higher growth and mitigates salt stress.

    Science.gov (United States)

    Khan, Abdul Latif; Hamayun, Muhammad; Khan, Sumera Afzal; Kang, Sang-Mo; Shinwari, Zabta Khan; Kamran, Muhammad; Ur Rehman, Shafiq; Kim, Jong-Guk; Lee, In-Jung

    2012-04-01

    Little is known about the role of endophytic fungi against abiotic stresses and isoflavonoids (IF) contents of soybean. In current study, we investigated the role of fungal endophytes on the growth of soybean under salt stress conditions. Pure cultures of nine endophytic fungi were isolated from the roots of field-grown soybean plants, and their culture filtrates were screened on Waito-C and Dongjin-byeo rice cultivars; for identification of plant growth promoting fungal strains. It was observed that fungal isolate GMC-2B significantly promoted the growth of both Waito-C and Dongjin-byeo. GMC-2B was later identified as a new strain of Metarhizium anisopliae LHL07 on the basis of 18S rDNA sequences and phylogenetic analysis. Metarhizium anisopliae LHL07 inoculated soybean plants recorded significantly higher shoot length, shoot fresh and dry biomass, chlorophyll contents, transpiration rate, photosynthetic rate and leaf area; under sodium chloride induced salt stress as compared to non-inoculated control plants. An elevated proline and reduced superoxide dismutase and malondialdehyde contents in M. anisopliae LHL07 inoculated soybean plants demonstrated mitigation of salt induced oxidative stress. Furthermore, reduced abscisic acid and elevated jasmonic acid contents in soybean plants confirmed that lesser stress was convened to M. anisopliae inoculated-plants under salinity stress. We also assessed the role of M. anisopliae interaction on IF biosynthesis of soybean, and found significantly higher IF contents in M. anisopliae inoculated soybean plants. In conclusion, endophytic fungal interactions with soybean can be beneficial to improve soybean quality and quantity under salt affected agricultural systems.

  16. Algal recycling enhances algal productivity and settleability in Pediastrum boryanum pure cultures.

    Science.gov (United States)

    Park, Jason B K; Craggs, Rupert J; Shilton, Andy N

    2015-12-15

    Recycling a portion of gravity harvested algae (i.e. algae and associated bacteria biomass) has been shown to improve both algal biomass productivity and harvest efficiency by maintaining the dominance of a rapidly-settleable colonial alga, Pediastrum boryanum in both pilot-scale wastewater treatment High Rate Algal Ponds (HRAP) and outdoor mesocosms. While algal recycling did not change the relative proportions of algae and bacteria in the HRAP culture, the contribution of the wastewater bacteria to the improved algal biomass productivity and settleability with the recycling was not certain and still required investigation. P. boryanum was therefore isolated from the HRAP and grown in pure culture on synthetic wastewater growth media under laboratory conditions. The influence of recycling on the productivity and settleability of the pure P. boryanum culture was then determined without wastewater bacteria present. Six 1 L P. boryanum cultures were grown over 30 days in a laboratory growth chamber simulating New Zealand summer conditions either with (Pr) or without (Pc) recycling of 10% of gravity harvested algae. The cultures with recycling (Pr) had higher algal productivity than the controls (Pc) when the cultures were operated at both 4 and 3 d hydraulic retention times by 11% and 38% respectively. Furthermore, algal recycling also improved 1 h settleability from ∼60% to ∼85% by increasing the average P. boryanum colony size due to the extended mean cell residence time and promoted formation of large algal bio-flocs (>500 μm diameter). These results demonstrate that the presence of wastewater bacteria was not necessary to improve algal productivity and settleability with algal recycling.

  17. Fermentation of residual glycerol by Clostridium acetobutylicum ATCC 824 in pure and mixed cultures.

    Science.gov (United States)

    Dams, Rosemeri I; Guilherme, Alexandre A; Vale, Maria S; Nunes, Vanja F; Leitão, Renato C; Santaella, Sandra T

    2016-12-01

    The aim of this research was to estimate the production of hydrogen, organic acids and alcohols by the strain of Clostridium acetobutylicum ATCC 824 using residual glycerol as a carbon source. The experiments were carried out in pure and mixed cultures in batch experiments. Three different sources of inocula for mixed culture were used. Ruminal liquid from goats and sludge collected from two upflow anaerobic sludge blanket reactors treating municipal wastewater and brewery effluent were tested for hydrogen, organic acids and alcohols production with or without C. acetobutylicum ATCC 824. The main detected end-products from the glycerol fermentation were hydrogen, organic acids (acetic, propionic, butyric and caproic) and alcohol (ethanol and 1,3-propanediol - 1,3PD). High hydrogen (0.44 mol H2/mol glycerol consumed) and 1,3PD (0.32 mol 1,3PD/mol glycerol consumed) yields were obtained when the strain C. acetobutylicum ATCC 824 was bioaugmented into the sludge from municipal wastewater using 5 g/L of glycerol. Significant concentrations of n-caproic acid were detected in the ruminal liquid when amended with C. acetobutylicum ATCC 824. The results suggest that glycerol can be used for the generation of H2, 1,3PD and n-caproic acid using C. acetobutylicum ATCC 824 as agent in pure or mixed cultures.

  18. Optimization of Differential Display of Prokaryotic mRNA: Application to Pure Culture and Soil Microcosms

    OpenAIRE

    Fleming, James T.; Yao, Wen-Hsiang; Sayler, Gary S.

    1998-01-01

    The differential display (DD) technique, which is widely used almost exclusively for eukaryotic gene discovery, was optimized to detect differential mRNA transcription from both pure-culture and soil-derived bacterial RNA. A model system which included toluene induction of todC1 in Pseudomonas putida F1 was used to optimize the procedure. At 24-h tod induction was determined to be approximately 8 × 107 transcripts/μg or 0.08% of the total mRNA. The primer concentration, primer length, anneali...

  19. [Effect of the fungicide captan on Azospirillum brasilense Cd in pure culture and associated with Setaria italica].

    Science.gov (United States)

    Di Ciocco, C A; Rodríguez Cáceres, E

    1997-01-01

    The effect of fungicide captan on growth and nitrogenase activity of Azospirillum brasilense Cd was studied in pure cultures and in association with foxtail millet (Setaria italica) cultivar Carapé under laboratory conditions. The 8 h growth in rotary shaker of A. brasilense was inhibited with 1 mg/l pure captan; however, after 4 days the differences diminished compared with the control without captan. Nitrogenase activity was affected with 10 mg/l but the differences were negligible after 48 h of growth. Root dry weight of inoculated plants was diminished by the treatment of foxtail millet cv. Carapé with captan. Inoculation with A. brasilense Cd increased shoot dry weight, but differences were significant only with respect to the control but not in relation to captan treatments.

  20. Leaching of pyrite by acidophilic heterotrophic iron-oxidizing bacteria in pure and mixed cultures

    Energy Technology Data Exchange (ETDEWEB)

    Bacelar-Nicolau, P.; Johnson, D.B. [Univ. of Wales, Bangor (United Kingdom). School of Biological Sciences

    1999-02-01

    Seven strains of heterotrophic iron-oxidizing acidophilic bacteria were examined to determine their abilities to promote oxidative dissolution of pyrite (FeS{sub 2}) when they were grown in pure cultures and in mixed cultures with sulfur-oxidizing Thiobacillus spp. Only one of the isolates (strain T-24) oxidized pyrite when it was grown in pyrite-basal salts medium. However, when pyrite-containing cultures were supplemented with 0.02% (wt/vol) yeast extract, most of the isolates oxidized pyrite, and one (strain T-24) promoted rates of mineral dissolution similar to the rates observed with the iron-oxidizing autotroph Thiobacillus ferroxidans. Pyrite oxidation by another isolate (strain T-21) occurred in cultures containing between 0.005 and 0.05% (wt/vol) yeast extract but was completely inhibited in cultures containing 0.5% yeast extract. Ferrous iron was also needed for mineral dissolution by the iron-oxidizing heterotrophs, indicating that these organisms oxidize pyrite via the indirect mechanism. Mixed cultures of three isolates (strains T-21, T-232, and T-24) and the sulfur-oxidizing autotroph Thiobacillus thiooxidans promoted pyrite dissolution; since neither strains T-21 and T-23 nor T. thiooxidans could oxidize this mineral in yeast extract-free media, this was a novel example of bacterial synergism. Mixed cultures of strains T-21 and T-23 and the sulfur-oxidizing mixotroph Thiobacillus acidophilus also oxidized pyrite but to a lesser extent than did mixed cultures containing T. thiooxidans. Pyrite leaching by strain T -23 grown in an organic compound-rich medium and incubated either shaken or unshaken was also assessed. The potential environmental significance of iron-oxidizing heterotrophs in accelerating pyrite oxidation is discussed.

  1. An IR study of pure and ion irradiated frozen formamide

    CERN Document Server

    Brucato, J R; Strazzulla, G; Baratta, Giuseppa A.; Brucato, John R.; Strazzulla, Giovanni

    2006-01-01

    Context. The chemical evolution of formamide (HCONH2), a molecule of astrobiological interest that has been tentatively identified in interstellar ices and in cometary coma, has been studied in laboratory under simulated astrophysical conditions such as ion irradiation at low temperature. Aims. To evaluate the abundances of formamide observed in space or in laboratory, the integrated absorbances for all the principal IR features of frozen amorphous pure formamide deposited at 20 K were measured. Further evidence that energetic processing of ices occurring in space is extremely relevant both to astrochemistry and to astrobiology has been found, showing that new molecular species are synthesized by ion irradiation at a low temperature. Methods. Pure formamide were deposited at 20 K and IR transmission spectra measured for different ice thicknesses. The ice thickness was derived by looking at the interference pattern (intensity versus time) of a He-Ne laser beam reflected at an angle of 45 deg by the vacuum-film...

  2. Mineral and iron oxidation at low temperatures by pure and mixed cultures of acidophilic microorganisms.

    Science.gov (United States)

    Dopson, Mark; Halinen, Anna-Kaisa; Rahunen, Nelli; Ozkaya, Bestamin; Sahinkaya, Erkan; Kaksonen, Anna H; Lindström, E Börje; Puhakka, Jaakko A

    2007-08-01

    An enrichment culture from a boreal sulfide mine environment containing a low-grade polymetallic ore was tested in column bioreactors for simulation of low temperature heap leaching. PCR-denaturing gradient gel electrophoresis and 16S rRNA gene sequencing revealed the enrichment culture contained an Acidithiobacillus ferrooxidans strain with high 16S rRNA gene similarity to the psychrotolerant strain SS3 and a mesophilic Leptospirillum ferrooxidans strain. As the mixed culture contained a strain that was within a clade with SS3, we used the SS3 pure culture to compare leaching rates with the At. ferrooxidans type strain in stirred tank reactors for mineral sulfide dissolution at various temperatures. The psychrotolerant strain SS3 catalyzed pyrite, pyrite/arsenopyrite, and chalcopyrite concentrate leaching. The rates were lower at 5 degrees C than at 30 degrees C, despite that all the available iron was in the oxidized form in the presence of At. ferrooxidans SS3. This suggests that although efficient At. ferrooxidans SS3 mediated biological oxidation of ferrous iron occurred, chemical oxidation of the sulfide minerals by ferric iron was rate limiting. In the column reactors, the leaching rates were much less affected by low temperatures than in the stirred tank reactors. A factor for the relatively high rates of mineral oxidation at 7 degrees C is that ferric iron remained in the soluble phase whereas, at 21 degrees C the ferric iron precipitated. Temperature gradient analysis of ferrous iron oxidation by this enrichment culture demonstrated two temperature optima for ferrous iron oxidation and that the mixed culture was capable of ferrous iron oxidation at 5 degrees C.

  3. Urodynamic study in women with pure stress urinary incontinence.

    Science.gov (United States)

    Valdevenito, J P; Águila, F; Naser, M; Manríquez, V; Wenzel, C; Díaz, J P

    2015-03-01

    To describe the results of urodynamic study in women with pure stress urinary incontinence symptoms, including the characteristics of the overactive detrusor. No other clinical assessments were taken into account. A retrospective study in women with urinary incontinence consecutively evaluated by urodynamic study. From a total of 710 women, only 108 (15%) with pure stress urinary incontinence symptoms were selected. Women with prior urinary incontinence surgery, pelvic organ prolapse (stage ≥iii), pelvic radiotherapy, using medication active on the lower urinary tract and neurological diseases were excluded. Infusion rate was 70 ml/min. Detrusor overactivity was induced only by cough. A standardized cough stress test with progressive cough intensity was carried out. Reference urodynamic values for stress incontinent women are described. Urodynamic stress incontinence was observed in 79 women (73.1%), detrusor overactivity in 4 (3.7%) and mixed urodynamic diagnosis in 15 (13.8%). Test was inconclusive in 10 patients (9.2%). Two women had detrusor overactivity incontinence (1.9%). One patient had detrusor overactivity induced by cough without urodynamic stress incontinence (0.9%). There was an association between detrusor overactivity and nocturia ≥2 (P=.002; odds ratio: 3.74; 95% confidence interval: 1.22-11.39). One woman had a bladder outlet obstruction (0.9%). In women with pure stress urinary incontinence, without knowing the outcome of other clinical assessments, urodynamic study can provide useful information to define the proper therapy. Copyright © 2014 AEU. Publicado por Elsevier España, S.L.U. All rights reserved.

  4. Optical cell separation from three-dimensional environment in photodegradable hydrogels for pure culture techniques.

    Science.gov (United States)

    Tamura, Masato; Yanagawa, Fumiki; Sugiura, Shinji; Takagi, Toshiyuki; Sumaru, Kimio; Matsui, Hirofumi; Kanamori, Toshiyuki

    2014-05-07

    Cell sorting is an essential and efficient experimental tool for the isolation and characterization of target cells. A three-dimensional environment is crucial in determining cell behavior and cell fate in biological analysis. Herein, we have applied photodegradable hydrogels to optical cell separation from a 3D environment using a computer-controlled light irradiation system. The hydrogel is composed of photocleavable tetra-arm polyethylene glycol and gelatin, which optimized cytocompatibility to adjust a composition of crosslinker and gelatin. Local light irradiation could degrade the hydrogel corresponding to the micropattern image designed on a laptop; minimum resolution of photodegradation was estimated at 20 µm. Light irradiation separated an encapsulated fluorescent microbead without any contamination of neighbor beads, even at multiple targets. Upon selective separation of target cells in the hydrogels, the separated cells have grown on another dish, resulting in pure culture. Cell encapsulation, light irradiation and degradation products exhibited negligible cytotoxicity in overall process.

  5. Effects of Arsenite Resistance on the Growth and Functional Gene Expression of Leptospirillum ferriphilum and Acidithiobacillus thiooxidans in Pure Culture and Coculture

    Directory of Open Access Journals (Sweden)

    Huidan Jiang

    2015-01-01

    Full Text Available The response of iron-oxidizing Leptospirillum ferriphilum YSK and sulfur-oxidizing Acidithiobacillus thiooxidans A01 to arsenite under pure culture and coculture was investigated based on biochemical characterization (concentration of iron ion and pH value and related gene expression. L. ferriphilum YSK and At. thiooxidans A01 in pure culture could adapt up to 400 mM and 800 mM As(III after domestication, respectively, although arsenite showed a negative effect on both strains. The coculture showed a stronger sulfur and ferrous ion oxidation activity when exposed to arsenite. In coculture, the pH value showed no significant difference when under 500 mM arsenite stress, and the cell number of At. thiooxidans was higher than that in pure culture benefiting from the interaction with L. ferriphilum. The expression profile showed that the arsenic efflux system in the coculture was more active than that in pure culture, indicating that there is a synergetic interaction between At. thiooxidans A01 and L. ferriphilum YSK. In addition, a model was proposed to illustrate the interaction between arsenite and the ars operon in L. ferriphilum YSK and At. thiooxidans A01. This study will facilitate the effective application of coculture in the bioleaching process by taking advantage of strain-strain communication and coordination.

  6. Effects of Arsenite Resistance on the Growth and Functional Gene Expression of Leptospirillum ferriphilum and Acidithiobacillus thiooxidans in Pure Culture and Coculture.

    Science.gov (United States)

    Jiang, Huidan; Liang, Yili; Yin, Huaqun; Xiao, Yunhua; Guo, Xue; Xu, Ying; Hu, Qi; Liu, Hongwei; Liu, Xueduan

    2015-01-01

    The response of iron-oxidizing Leptospirillum ferriphilum YSK and sulfur-oxidizing Acidithiobacillus thiooxidans A01 to arsenite under pure culture and coculture was investigated based on biochemical characterization (concentration of iron ion and pH value) and related gene expression. L. ferriphilum YSK and At. thiooxidans A01 in pure culture could adapt up to 400 mM and 800 mM As(III) after domestication, respectively, although arsenite showed a negative effect on both strains. The coculture showed a stronger sulfur and ferrous ion oxidation activity when exposed to arsenite. In coculture, the pH value showed no significant difference when under 500 mM arsenite stress, and the cell number of At. thiooxidans was higher than that in pure culture benefiting from the interaction with L. ferriphilum. The expression profile showed that the arsenic efflux system in the coculture was more active than that in pure culture, indicating that there is a synergetic interaction between At. thiooxidans A01 and L. ferriphilum YSK. In addition, a model was proposed to illustrate the interaction between arsenite and the ars operon in L. ferriphilum YSK and At. thiooxidans A01. This study will facilitate the effective application of coculture in the bioleaching process by taking advantage of strain-strain communication and coordination.

  7. Fermentative hydrogen production by Clostridium butyricum CWBI1009 and Citrobacter freundii CWBI952 in pure and mixed cultures

    Directory of Open Access Journals (Sweden)

    Beckers, L.

    2010-01-01

    Full Text Available This paper investigates the biohydrogen production by two mesophilic strains, a strict anaerobe (Clostridium butyricum CWBI1009 and a facultative anaerobe (Citrobacter freundii CWBI952. They were cultured in pure and mixed cultures in serum bottles with five different carbon sources. The hydrogen yields of pure C. freundii cultures ranged from 0.09 molH2.molhexose-1 (with sucrose to 0.24 molH2.molhexose-1 (with glucose. Higher yields were obtained by the pure cultures of Cl. butyricum ranging from 0.44 molH2.molhexose-1 (with sucrose to 0.69 molH2.molhexose-1 (with lactose. This strain also fermented starch whereas C. freundii did not. However, it consumed the other substrates faster and produced hydrogen earlier than Cl. butyricum. This ability has been used to promote the growth conditions of Cl. butyricum in co-culture with C. freundii, since Cl. butyricum is extremely sensitive to the presence of oxygen which strongly inhibits H2 production. This approach could avoid the addition of any expensive reducing agents in the culture media such as L-cysteine since C. freundii consumes the residual oxygen. Thereafter, co-cultures with glucose and starch were investigated: hydrogen yields decreased from 0.53 molH2.molhexose-1 for pure Cl. butyricum cultures to 0.38 molH2.molhexose-1 for mixed culture with glucose but slightly increased with starch (respectively 0.69 and 0.73 molH2.molhexose-1. After 48 h of fermentation, metabolites analysis confirmed with microbial observation, revealed that the cell concentration of C. freundii dramatically decreased or was strongly inhibited by the development of Cl. butyricum.

  8. Mercury Stable Isotopic Composition of Monomethylmercury in Estuarine Sediments and Pure Cultures of Mercury Methylating Bacteria

    Science.gov (United States)

    Janssen, S.; Johnson, M. W.; Barkay, T.; Blum, J. D.; Reinfelder, J. R.

    2014-12-01

    Tracking monomethylmercury (MeHg) from its source in soils and sediments through various environmental compartments and transformations is critical to understanding its accumulation in aquatic and terrestrial food webs. Advances in the field of mercury (Hg) stable isotopes have allowed for the tracking of discrete Hg sources and the examination of photochemical and bacterial transformations. Despite analytical advances, measuring the Hg stable isotopic signature of MeHg in environmental samples or laboratory experiments remains challenging due to difficulties in the quantitative separation of MeHg from complex matrices with high concentrations of inorganic Hg. To address these challenges, we have developed a MeHg isolation method for sediments and bacterial cultures which involves separation by gas chromatography. The MeHg eluting from the GC is passed through a pyrolysis column and purged onto a gold amalgam trap which is then desorbed into a final oxidizing solution. A MeHg reference standard carried through our separation process retained its isotopic composition within 0.02 ‰ for δ202Hg, and for native estuarine sediments, MeHg recoveries were 80% to 100%. For sediment samples from the Hackensack and Passaic Rivers (New Jersey, USA), δ202Hg values for MeHg varied from -1.2 to +0.58 ‰ (relative to SRM 3133) and for individual samples were significantly different from that of total Hg (-0.38 ± 0.06 ‰). No mass independent fractionation was observed in MeHg or total Hg from these sediments. Pure cultures of Geobacter sulfurreducens, grown under fermentative conditions showed preferential enrichment of lighter isotopes (lower δ202Hg) during Hg methylation. The Hg stable isotope signatures of MeHg in sediments and laboratory methylation experiments will be discussed in the context of the formation and degradation of MeHg in the environment and the bioaccumulation of MeHg in estuarine food webs.

  9. Degradation of the Ferric Chelate of EDTA by a Pure Culture of an Agrobacterium sp.

    Science.gov (United States)

    Lauff, J J; Steele, D B; Coogan, L A; Breitfeller, J M

    1990-11-01

    A pure culture of an Agrobacterium sp. (deposited as ATCC 55002) that mineralizes the ferric chelate of EDTA (ferric-EDTA) was isolated by selective enrichment from a treatment facility receiving industrial waste containing ferric-EDTA. The isolate grew on ferric-EDTA as the sole carbon source at concentrations exceeding 100 mM. As the degradation proceeded, carbon dioxide, ammonia, and an unidentified metabolite(s) were produced; the pH increased, and iron was precipitated from solution. The maximum rate of degradation observed with sodium ferric-EDTA as the substrate was 24 mM/day. At a substrate concentration of 35 mM, 90% of the substrate was degraded in 3 days and 70% of the associated chemical oxygen demand was removed from solution. Less than 15% of the carbon initially present was incorporated into the cell mass. Significant growth of this strain was not observed with uncomplexed EDTA as the sole carbon source at comparable concentrations; however, the ferric chelate of propylenediaminetetraacetic acid (ferric-PDTA) did support growth.

  10. Degradation of the ferric chelate of EDTA by a pure culture of an Agrobacterium sp

    Energy Technology Data Exchange (ETDEWEB)

    Lauff, J.J.; Coogan, L.A.; Breitfeller, J.M. (Genencor International, Rochester, NY (USA)); Steele, D.B. (Auburn Univ., AL (USA))

    1990-11-01

    A pure culture of an Agrobacterium sp. (deposited as ATCC 55002) the mineralizes the ferric chelate of EDTA (ferric-EDTA) was isolated by selective enrichment from a treatment facility receiving industrial waste containing ferric-EDTA. The isolated grew on ferric-EDTA as the sole carbon source at concentrations exceeding 100 mM. As the degradation proceeded, carbon dioxide, ammonia, and an unidentified metabolite(s) were produced; the pH increased, and iron was precipitated from solution. The maximum rate of degradation observed with sodium ferric-EDTA as the substrate was 24 mM/day. At a substrate concentration of 35 mM, 90% of the substrate was degraded in 3 days and 70% of the associated chemical oxygen demand was removed from solution. Less than 15% of the carbon initially present was incorporated into the cell mass. Significant growth of this strain was not observed with uncomplexed EDTA as the sole carbon source at comparable concentrations; however, the ferric chelate of propylenediaminetetraacetic acid (ferric-PDTA) did support growth.

  11. Protein improvement in Gari by the use of pure cultures of microorganisms involved in the natural fermentation process.

    Science.gov (United States)

    Ahaotu, I; Ogueke, C C; Owuamanam, C I; Ahaotu, N N; Nwosu, J N

    2011-10-15

    The ability of microorganisms involved in cassava mash fermentation to produce and improve protein value by these microorganisms during fermentation was studied. Standard microbiological procedures were used to isolate, identify and determine the numbers of the organisms. Alcaligenes faecalis, Lactobacillus plantarum, Bacillus subtilis, Leuconostoc cremoris, Aspergillus niger, A. tamari, Geotrichum candidum and Penicillium expansum were isolated and identified from cassava waste water while standard analytical methods were used to determine the ability of the isolates to produce linamarase and the proximate composition, pH and titrable acidity of the fermenting mash. The linamarase activity of the isolates ranged from 0.0416 to 0.2618 micromol mL(-1) nmol(-1). Bacillus subtilis, A. niger, A. tamari and P. expansum did not express any activity for the enzyme. Protein content of mash fermented with mixed fungal culture had the highest protein value (15.4 mg/g/dry matter) while the raw cassava had the least value (2.37 mg/g/dry matter). The naturally fermented sample had the least value for the fermented samples (3.2 mg/g/dry matter). Carbohydrate and fat contents of naturally fermented sample were higher than values obtained from the other fermented samples. Microbial numbers of the sample fermented with mixed bacterial culture was highest and got to their peak at 48 h (57 x 10(8) cfu g(-1)). pH decreased with increase in fermentation time with the mash fermented by the mixed culture of fungi having the lowest pH of 4.05 at the end of fermentation. Titrable acidity increased with increase in fermentation time with the highest value of 1.32% at 96 h of fermentation produced by the mixed culture of fungi. Thus fermentation with the pure cultures significantly increased the protein content of mash.

  12. Biodegradation of Synthetic Polyesters (BTA and PCL with Natural Flora in Soil Burial and Pure Cultures under Ambient Temperature

    Directory of Open Access Journals (Sweden)

    Mona K. Gouda

    2012-03-01

    Full Text Available The aim of this study was to study the biodegradation of two synthetic polyesters, one aliphaticaromatic (1, 4-butanediol, terephthalic-adipic acid, BTA and the other aliphatic (poly (,-caprolactone, PCL, under different soil types (canal shore soil, garden soil, compost and Peat moss, respectively, as well as using locally isolated cultures at ambient temperature. The results showed that the BTA films buried in canal shore and garden soil were degraded faster than that in the other soils. After six weeks about 90, 88 and 80% were degraded in garden, canal shore soil and compost respectively, while only 52% were degraded in Peat moss. On the other hand, 95 and 93% weight loss was obtained for PCL films buried for three weeks in canal shore and garden soil respectively. The Scanning Electron Microscope photos confirm the results of weight loss and revealed the presence of cracks and fungal growth on films buried in different soils. The results with pure cultures, especially with Fusarium solani, also confirmed the biodegradability of two polyesters under ambient temperature. Finally, it could be concluded that both synthetic polyester are degradable under ambient conditions.

  13. Optimization of Differential Display of Prokaryotic mRNA: Application to Pure Culture and Soil Microcosms

    Science.gov (United States)

    Fleming, James T.; Yao, Wen-Hsiang; Sayler, Gary S.

    1998-01-01

    The differential display (DD) technique, which is widely used almost exclusively for eukaryotic gene discovery, was optimized to detect differential mRNA transcription from both pure-culture and soil-derived bacterial RNA. A model system which included toluene induction of todC1 in Pseudomonas putida F1 was used to optimize the procedure. At 24-h tod induction was determined to be approximately 8 × 107 transcripts/μg or 0.08% of the total mRNA. The primer concentration, primer length, annealing temperature, and template, deoxynucleoside triphosphate, and MgCl2 concentrations were varied to optimize amplification of a todC1 fragment. The limit of detection of todC1 by DD was found to be 0.015 ng of total RNA template or approximately 103 transcripts. Once optimized, a todC1C2 gene fragment from P. putida F1 RNA was detected by using an arbitrary primer for the reverse transcriptase step in conjunction with the same arbitrary primer and a Shine-Dalgarno primer in the PCR. To verify the results, an arbitrary primer was used to detect recovery of a new salicylate-inducible naphthalene dioxygenase in Burkholderia cepacia JS150. The method was then used to detect mRNA induction in both inoculated and uninoculated toluene-induced soil microcosms. Several putative differentially expressed partial gene sequences obtained from the uninoculated microcosms were examined, and one novel fragment was found to be differentially expressed. PMID:9758787

  14. Factors Affecting Volatile Phenol Production During Fermentations with Pure and Mixed Cultures of Dekkera bruxellensis and Saccharomyces cerevisiae

    Directory of Open Access Journals (Sweden)

    Janez Kosel

    2014-01-01

    Full Text Available The paper examines the impact of ethanol, and hidroxycinnamic and vinylphenol precursors on the production of volatile phenols in fermentations of mixed and pure cultures of yeasts Saccharomyces cerevisiae and Dekkera bruxellensis. Three different D. bruxellensis strains were examined and they all showed a unique volatile phenol production pattern in the fermentations of pure and mixed cultures. Generally, the results showed that in mixed culture fermentations less vinylphenols and more ethylphenols were produced in comparison with D. bruxellensis pure culture fermentations. Vinylphenol precursors significantly inhibited the growth of S. cerevisiae and the production of ethylphenols. Nevertheless, it was found that D. bruxellensis genes encoding for enzymes coumaric acid decarboxylase (CAD and vinylphenol reductase (VPR are more responsive to vinylphenol precursors in comparison with hidroxycinnamic acids. Consequently, higher concentrations of vinylphenols in the cell were found to be more cytotoxic than hidroxycinnamic acids. In general, low ethanol concentrations induced higher production of volatile phenols by S. cerevisiae and D. bruxellensis. This was confirmed with the expression pattern of gene encoding for CAD of D. bruxellensis.

  15. The efficacy of X-ray does on murine norovirus-1 (MNV-1) in pure culture, half-shell oyster, salmon sushi, and tuna salad

    Science.gov (United States)

    In this investigation, we determined the efficacy of X-ray doses on reducing a human norovirus (HuNoV) surrogate [murine norovirus-1 (MNV-1)] in pure culture, half-shell oyster, salmon sushi and tuna salad. The pure culture (phosphate-buffer saline, pH 7.4), half-shell oyster, salmon sushi and tuna ...

  16. Oxidation of acetate through reactions of the citric acid cycle by Geobacter sulfurreducens in pure culture and in syntrophic coculture

    OpenAIRE

    2000-01-01

    Geobacter sulfurreducens strain PCA oxidized acetate to CO2 via citric acid cycle reactions during growth with acetate plus fumarate in pure culture, and with acetate plus nitrate in coculture with Wolinella succinogenes. Acetate was activated by succinyl-CoA:acetate CoA-transferase and also via acetate kinase plus phosphotransacetylase. Citrate was formed by citrate synthase. Soluble isocitrate and malate dehydrogenases reduced NADP+ and NAD+, respectively. Oxidation of 2-oxoglutarate was me...

  17. Studies on kinetics and thermodynamics of biomachining pure copper

    Institute of Scientific and Technical Information of China (English)

    张德远; 李雅芹

    1999-01-01

    The kinetics and thermodynamics of biomachining pure copper by Thiobacillus ferrooxidans were quantitatively analyzed. The kinetic effect of bacteria on the ion-cycle between Fe2+ and Fe3+ and the thermodynamic effect of hydrolysis on the changes of pH in biomachining processes were expounded through six series of experiment. Finally a model of ion-cycle in the biomachining processes was proposed, and the measures for maintaining the stable equilibrium of the ion-cycle were also discussed.

  18. A study of purely astrometric selection of extragalactic point sources

    CERN Document Server

    Heintz, Kasper E; Høg, Erik

    2015-01-01

    Selection of extragalactic point sources, e.g. QSOs, is often hampered by significant selection effects causing existing samples to have rather complex selection functions. We explore whether a purely astrometric selection of extragalactic point sources, e.g. QSOs, is feasible with the ongoing Gaia mission. Such a selection would be interesting as it would be unbiased in terms of colours of the targets and hence would allow selection also with colours in the stellar sequence. We have analyzed a total of 18 representative regions of the sky by using \\textit{GUMS}, the simulator prepared for ESAs Gaia mission, both in the range of $12\\le G \\le 20$ mag and $12\\le G \\le 18$ mag. For each region we determine the density of apparently stationary stellar sources, i.e. sources for which Gaia cannot measure a significant proper motion. The density is contrasted with the density of extragalactic point sources, e.g. QSOs, in order to establish in which celestial directions a pure astrometric selection is feasible. When ...

  19. The evaluation of kefir pure culture starter: Liquid-core capsule entrapping microorganisms isolated from kefir grains.

    Science.gov (United States)

    Wang, Liang; Zhong, Hao; Liu, Keying; Guo, Aizhen; Qi, Xianghui; Cai, Meihong

    2016-10-01

    The main purpose of this study was to develop a pure culture starter for producing kefir. In order to accomplish starter recycling, yeasts (Kluyveromyces marxianus strain, Pichia kudriavzevii clone), lactic acid bacteria (Lactobacillus kefiri strain F4Aa, Lactobacillus kefiri strain NM131-7, Lactobacillus kefiri strain NM132-3, Lactobacillus kefiri strain NM180-3, respectively), and acetic acid bacteria (Acetobacter lovaniensis strain) were entrapped in liquid core capsules based on the distribution ratio in kefir grains. The microbiological, antimicrobial, and chemical properties of kefir made with capsules (M) and kefir grains (K) were measured and compared. According to the results of plate counts in different selective medium, the number of yeasts and bacteria in the liquid core capsules gradually increased and stabilized after eight fermentation cycles. The results of gas chromatography-mass spectrometry showed that almost all the aroma components existed in the two type of kefir, except the ethyl lactate. There was no significant difference in alcohol content, protein content, and fat content, except the acidity and sugar content. Water holding capacity of kefir K was higher than kefir M. There were 14 same free amino acids in kefir M and kefir K, and the content of most free amino acids was similar. In antimicrobial test, there was no significant difference in both kefirs. © The Author(s) 2016.

  20. Gibberellin production and plant growth promotion from pure cultures of Cladosporium sp. MH-6 isolated from cucumber (Cucumis sativus L.).

    Science.gov (United States)

    Hamayun, Muhammad; Khan, Sumera Afzal; Khan, Abdul Latif; Rehman, Gauhar; Kim, Youn-Ha; Iqbal, Ilyas; Hussain, Javid; Sohn, Eun-Young; Lee, In-Jung

    2010-01-01

    Gibberellin (GA) production by soil fungi has received little attention, although substantial work has been carried out on other aspects of plant growth promoting fungi (PGPF). In our studies we investigated GA production and growth-promoting capacity of a novel fungal strain isolated from the roots of soil-grown cucumber. Pure cultures of 19 endophytic fungi were tested for shoot length promotion of Waito-C rice to identify the GA production capacity of these fungal isolates. Isolate MH-6 significantly increased shoot length (12.9 cm) of Waito-C, in comparison to control treatments. Bioassay with culture filtrate (CF) of MH-6 also significantly promoted growth attributes of cucumber plants. Analysis of MH-6 CF showed the presence of physiologically active (GA1, 1.97 ng/mL; GA3, 5.18 ng/mL; GA4, 13.35 ng/mL and GA7, 2.4 ng/ mL) in conjunction with physiologically inactive (GA9 [0.69 ng/mL], GA12 [0.24 ng/mL], GA15 [0.68 ng/ mL, GA19 [1.94 ng/mL and GA20 [0.78 ng/mL]) gibberellins. The CF of MH-6 produced greater amounts of GA3, GA4, GA7 and GA19 than wild type Fusarium fujikuroi, a fungus known for high production of GA. The fungal isolate MH-6 was identified as a new strain of Cladosporium sp. on the basis of sequence homology (99%) and phylogenetic analysis of 18S rDNA sequence.

  1. Pure erythrocytosis: reappraisal of a study of 51 cases

    Energy Technology Data Exchange (ETDEWEB)

    Najean, Y.; Triebel, F.; Dresch, C.

    1981-01-01

    Fifty-one cases of pure, primary erythrocytosis were identified and followed at Hopital Saint-Louis, Paris, and compared with 350 cases of polycythemia vera (PV) observed during the same period. At the initial evaluation, these cases did not differ from PV cases with respect to age, sex ratio, degree of red cell volume increase, and clinical symptoms. They did differ by the absence of splenomegaly, granulocytosis and thrombocytosis. At a late stage of evolution only a few cases developed classical criteria of PV. From this group of apparently homogeneous cases, two subgroups evolved. Sixty percent of the cases were highly responsive to myelosuppression with /sup 32/P. The median duration of the first remission was greater than five years, the mean yearly dose of /sup 32/P was very low, and there was a low incidence of complications. The other group (40% of cases) was relatively resistant to myelosuppressive agents. The development of better methods of investigate this disorder might help in discriminating these two groups from both an etiological and pathophysiological viewpoint. The thromboembolic risk of these diseases suggests that myelosuppressive therapy should be utilized in older patients with higher risk of vascular accidents, reserving phlebotomy for younger patients and those who are shown to be resistant to /sup 32/P therapy.

  2. Simple screening method for molds producing intracellular mycotoxins in pure cultures.

    Science.gov (United States)

    Filtenborg, O; Frisvad, J C; Svendsen, J A

    1983-02-01

    A simple screening method for molds producing the intracellular mycotoxins brevianamide A, citreoviridin, cyclopiazonic acid, luteoskyrin, penitrem A, roquefortine C, sterigmatocystin, verruculogen, viomellein, and xanthomegnin was developed. After removing an agar plug from the mold culture, the mycelium on the plug is wetted with a drop of methanol-chloroform (1:2). By this treatment the intracellular mycotoxins are extracted within seconds and transferred directly to a thin-layer chromatography plate by immediately placing the plug on the plate while the mycelium is still wet. After removal of the plug, known thin-layer chromatographic procedures are carried out. The substrate (Czapek yeast autolysate agar) and growth conditions (25 degrees C for 7 days) used by Penicillium taxonomists proved suitable for the production of the mycotoxins investigated when 60 known toxigenic isolates and 865 cultures isolated from foods and feedstuffs were tested with this screening method.

  3. Cultural Studies in Turkey: The State of the Art

    Directory of Open Access Journals (Sweden)

    Gönül Pultar

    2013-06-01

    Full Text Available Recent socio-political developments have rendered cultural studies of the Repub-lic of Turkey an ever-widening field of study, as they lead apparently to a proba-ble paradigm shift in a society that was once thought to be purely Western-oriented. The analysis of this transformation is before all else a cultural studies task. Accordingly, this paper has two aims: one, to make a a brief survey of cultur-al studies work that has been done so far in Turkey; and two, draw attention to the various problems encountered by the instruction and practice of cultural stud-ies in the country.

  4. Extraction and characterization of bound extracellular polymeric substances from cultured pure cyanobacterium (Microcystis wesenbergii).

    Science.gov (United States)

    Liu, Lizhen; Qin, Boqiang; Zhang, Yunlin; Zhu, Guangwei; Gao, Guang; Huang, Qi; Yao, Xin

    2014-08-01

    Preliminary characterization of bound extracellular polymeric substances (bEPS) of cyanobacteria is crucial to obtain a better understanding of the formation mechanism of cyanobacterial bloom. However, the characterization of bEPS can be affected by extraction methods. Five sets (including the control) of bEPS from Microcystis extracted by different methods were characterized using three-dimensional excitation and emission matrix (3DEEM) fluorescence spectroscopy combined chemical spectrophotometry; and the characterization results of bEPS samples were further compared. The agents used for extraction were NaOH, pure water and phosphate buffered saline (PBS) containing cationic exchange resins, and hot water. Extraction methods affected the fluorescence signals and intensities in the bEPS. Five fluorescence peaks were observed in the excitation and emission matrix fluorescence spectra of bEPS samples. Two peaks (peaks T₁ and T₂) present in all extractions were identified as protein-like fluorophores, two (peaks A and C) as humic-like fluorophores, and one (peak E) as a fulvic-like substance. Among these substances, the humic-like and fulvic-like fluorescences were only seen in the bEPS extracted with hot water. Also, NaOH solution extraction could result in strong fluorescence intensities compared to the other extraction methods. It was suggested that NaOH at pH10.0 was the most appropriate method to extract bEPS from Microcystis. In addition, dialysis could affect the yields and characteristics of extracted bEPS during the determination process. These results will help us to explore the issues of cyanobacterial blooms.

  5. Ultratraces of carotenes in tomato purees : HPLC-TLS study

    NARCIS (Netherlands)

    Luterotti, S.; Markovic, K.; Franko, M.; Bicanic, D.D.; Vahcic, N.; Doka, O.

    2003-01-01

    The present study was designed to provide information about (i) the profile of carotene pigments and (ii) trace quantities of lycopene and -carotene left in tomato purées. The ultrasensitive method comprising HPLC and thermal lens spectrometric (TLS) detection enabled us to detect as low as 0.3 and

  6. Degradation and mineralization of wheat straw by some cellulolytic fungi in pure cultures

    Energy Technology Data Exchange (ETDEWEB)

    Omar, S.A. (Botany Dept., Faculty of Science, Assiut Univ. (Egypt))

    1994-01-01

    Decomposition and mineralization of wheat straw inoculated with 8 cellulolytic fungi was investigated in sand culture. Aspergillus fumigatus and Stachybotrys chartarum showed a great potential to degrade wheat straw and weight loss of straw was 35.3 and 27.5% of initial weight after 63 days incubation, respectively. CO[sub 2] production was highest after 7 and 14 days for all tested fungi suggesting that the main contribution to CO[sub 2] production arose from the water-soluble fraction which was decomposed rapidly. Carbon solubilization from wheat straw occurred, to some extent, in a manner similar to that of straw decomposition. Also, S. chartarum and A. fumigatus solubilized considerable amounts of straw Nitrogen but Gibberella fujikuroi was the best nitrogen dissolving organism. (orig.)

  7. Anaerobic degradation of o-phenylphenol by mixed and pure cultures

    Energy Technology Data Exchange (ETDEWEB)

    Sembiring, T.; Winter, J.

    1989-07-01

    An anaerobic enrichment culture that degrade 0.4 mmol/l per day of o-phenylphenol was selected from sediment of a waste water pond of a sugar factory. From the consortium an o-phenylphenol-degrading bacterium, strain B10, was isolated. Strain B10 could not degrade other aromatic substances, including phenylacetic acid, benzoate, o-hydroxybenzoate, p-hydroxybenzoate and phenol. Best growth was observed with glucose, pyruvate, lactate, methanol and H/sub 2//CO/sub 2/ as substrates, o-Phenylphenol was slowly degraded if supplied as the only carbon source and was cometabolized in the presence of >5 mmol/l glucose. Strain B10 has not yet been assigned to a known species or family.

  8. Ab-initio study of thermal expansion in pure graphene

    Energy Technology Data Exchange (ETDEWEB)

    Mann, Sarita; Kumar, Ranjan; Jindal, V. K., E-mail: jindal@pu.ac.in [Department of Physics, Panjab University, Chandigarh-160014 (India); Rani, Pooja [D.A.V. College, Sec-10, Chandigarh-160010 (India)

    2016-05-23

    Graphene is a zero band gap semiconductor with exceptionally high thermal conductivity. The electronic properties having been studied, therole of phonon in contributing to thermal expansion, thermal conductivity and other thermodynamic properties, is required to be investigated. This paper focuses more on thermal expansion. Some others results like phonon dispersion, Grüneisenparameters and bulk modulus,which are essential to estimation of thermal expansion, are also presented. The dynamical matrix was calculated using VASP code using both DFT and DFPT and the phonon frequencies were calculated using phonopy code under harmonic approximation. The linear thermal expansion coefficient of graphene is found to be strongly dependent on temperature but remains negative upto 470 K and positive thereafter, with a room temperature value of −1.44×10{sup −6}. The negative expansion coefficient is very interesting and is found to be in conformity with experimental as well as with recent theoretical estimates. There is only qualitative agreement of our results with experimental data and motivates further investigation, primarily on the high negative values of Grüneisen parameters.

  9. Ab-initio study of thermal expansion in pure graphene

    Science.gov (United States)

    Mann, Sarita; Rani, Pooja; Kumar, Ranjan; Jindal, V. K.

    2016-05-01

    Graphene is a zero band gap semiconductor with exceptionally high thermal conductivity. The electronic properties having been studied, therole of phonon in contributing to thermal expansion, thermal conductivity and other thermodynamic properties, is required to be investigated. This paper focuses more on thermal expansion. Some others results like phonon dispersion, Grüneisenparameters and bulk modulus,which are essential to estimation of thermal expansion, are also presented. The dynamical matrix was calculated using VASP code using both DFT and DFPTand the phonon frequencies were calculated using phonopy code under harmonic approximation. The linear thermal expansion coefficient of graphene is found to be strongly dependent on temperature but remains negative upto 470 K and positive thereafter, with a room temperature value of -1.44×10-6. The negative expansion coefficient is very interesting and is found to be in conformity with experimental as well as with recent theoretical estimates. There is only qualitative agreement of our results with experimental data and motivates further investigation, primarily on the high negative values of Grüneisen parameters.

  10. Comparative Study on Mechanical Properties between Pure and Recycled Polypropylenes

    Directory of Open Access Journals (Sweden)

    Ariadne L. Juwono

    2010-04-01

    Full Text Available Polypropylene (PP is one type of thermoplastics that is widely used in our daily activities. A combination of the high demand and the easiness of recycling process, the recycled PP has been generally applied. In this study, the structure and the mechanical properties of the as-received PPs, recycled PPs, and commercial recycled PPs were compared, especially for cloth hanger application. DSC test results showed that recycling process did not cause a significant change to the material's melting point, which stayed in a range of 160-163 oC. Meanwhile, FTIR test results showed that the commercial recycled PPs contained of Polyethylene (PE, which was not found in the as-received and the recycled PPs. Tensile and hardness tests demonstrated that there were no significant differences between the as-received and recycled PPs. In contrast, tensile test results of the commercial recycled PPs showed that the tensile strength, Young modulus and strain-at-break were lower than those of the as-received PPs by 22.1%, 8.1% and 65.7% respectively. The hardness test results of the commercial recycled PPs showed that the recycling process had a little effect on the material's hardness. These facts were supported by SEM observation on the surface that the contour of the commercial recycled PPs was relatively flatter and had smaller grain size than those of the as-received PPs. This indicated that the commercial recycled PPs were more brittle compared to the recycled PPs. To conclude, the recycled PPs have similar properties to the as-received PPs so that recycled PPs are suitable to be applied as cloth hanger application.

  11. US Cultural Studies: Oxymoron?

    Directory of Open Access Journals (Sweden)

    Marcus Breen

    2013-08-01

    Full Text Available This essay began in 1999 as an attempt to update my perceptions and experiences as a resident alien in the USA. Written expressly for what was then The UTS Review, it was intended to follow up my earlier piece published in 1997, ‘The Cultural Studies Thing You Do: In the USA after Sokal’. Just as the challenges initiated by that ‘affair’ seem to have evaporated, or been absorbed into the apparatus of systemic reinvention in the US academy, I am reinventing my correspondence.

  12. Studying Popular Culture in Japan

    DEFF Research Database (Denmark)

    Moeran, Brian

    This working paper – written for inclusion as a chapter on Japanese society, to be published in Chinese by the Beijing University of Foreign Studies later in 2011 – looks at popular culture as a form of cultural production. It argues for the need to study popular cultural forms like advertisement...

  13. Fermentative Hydrogen Production by Pure Culture with a New H2-producing Anaerobe

    Institute of Scientific and Technical Information of China (English)

    LI Yong-feng; REN Nan-qi; YANG Chuan-ping; XU Jing-li

    2006-01-01

    As a new clean energy source, the utilization and demand for hydrogen fuel are rapidly increasing. The integrated process of wastewater treatment of DESAR and energy recovery was developed in the studies. A new hydrogen anaerobe was isolated from the activated sludge. The optimal glucose concentration and the optimal initial pH were 12.0 g/L and 5. 5 respectively. The optimum C/N of the growth and hydrogen production in Rennanqilyf3 was (3.0 ~3.5): 1. The integrated process between DESAR system and biohydrogen production will be an important progress on energy recovery of DESAR system.

  14. Doing Cultural Studies

    DEFF Research Database (Denmark)

    du Gay, Paul; Hall, Stuart; Janes, Linda

    to the past - unpack the key concepts of contemporary culture, such as mobility and materiality - look with fresh eyes at today's media world and the cultural practices it gives rise to - practice their critical skills with up-to-date exercises and activities This book remains the perfect 'how to...

  15. Doing Cultural Studies

    DEFF Research Database (Denmark)

    du Gay, Paul; Hall, Stuart; Janes, Linda

    What does the Walkman have to do with the 21st century? The long-awaited second edition of this classic textbook takes students on a journey between past and present, giving them the skills do to cultural analysis along the way. Through the notion of the 'circuit of culture', this book teaches st...

  16. The response of dark septate endophytes (DSE) to heavy metals in pure culture.

    Science.gov (United States)

    Ban, Yihui; Tang, Ming; Chen, Hui; Xu, Zhouying; Zhang, Haihan; Yang, Yurong

    2012-01-01

    Dark septate endophytes (DSE) occur widely in association with plants exposed to heavy metal stress. However, little is known about the response of DSE exposed to heavy metals. In this study, five DSE were isolated from the roots of Astragalus adsurgens Pall. seedlings growing on lead-zinc mine tailings in China. Based on morphological characteristics and DNA sequence analyses, the isolates were identified as Gaeumannomyces cylindrosporus, Paraphoma chrysanthemicola, Phialophora mustea, Exophiala salmonis, and Cladosporium cladosporioides. G. cylindrosporus was selected to explore responses to Pb stress. Scanning electron microscopic observations of G. cylindrosporus grown on solid medium revealed curling of hyphae and formation of hyphal coils in response to Pb. In contrast, in liquid medium, hyphae became thick and swollen with an increase in Pb (II) concentration. We interpret that these changes are related to the variation in cell wall components. We also demonstrated that fungal melanin content increased with the addition of Pb(II). Melanin, as an important component in the cell wall, is known to be an essential antioxidant responsible for decreasing heavy metal toxicity. We also measured the total soluble protein content and glutathione (GSH) concentrations in G. cylindrosporus and found that they initially increased and then decreased with the increase of Pb(II) concentrations. The antioxidant enzyme activities were also examined, and the results showed that superoxide dismutase (SOD) activity was significantly positively correlated with Pb(II) concentrations (r = 0.957, P<0.001). Collectively, our observations indicate that the intracellular antioxidant systems, especially fungal melanin, play an important role in abating the hazards of heavy metals.

  17. The response of dark septate endophytes (DSE to heavy metals in pure culture.

    Directory of Open Access Journals (Sweden)

    Yihui Ban

    Full Text Available Dark septate endophytes (DSE occur widely in association with plants exposed to heavy metal stress. However, little is known about the response of DSE exposed to heavy metals. In this study, five DSE were isolated from the roots of Astragalus adsurgens Pall. seedlings growing on lead-zinc mine tailings in China. Based on morphological characteristics and DNA sequence analyses, the isolates were identified as Gaeumannomyces cylindrosporus, Paraphoma chrysanthemicola, Phialophora mustea, Exophiala salmonis, and Cladosporium cladosporioides. G. cylindrosporus was selected to explore responses to Pb stress. Scanning electron microscopic observations of G. cylindrosporus grown on solid medium revealed curling of hyphae and formation of hyphal coils in response to Pb. In contrast, in liquid medium, hyphae became thick and swollen with an increase in Pb (II concentration. We interpret that these changes are related to the variation in cell wall components. We also demonstrated that fungal melanin content increased with the addition of Pb(II. Melanin, as an important component in the cell wall, is known to be an essential antioxidant responsible for decreasing heavy metal toxicity. We also measured the total soluble protein content and glutathione (GSH concentrations in G. cylindrosporus and found that they initially increased and then decreased with the increase of Pb(II concentrations. The antioxidant enzyme activities were also examined, and the results showed that superoxide dismutase (SOD activity was significantly positively correlated with Pb(II concentrations (r = 0.957, P<0.001. Collectively, our observations indicate that the intracellular antioxidant systems, especially fungal melanin, play an important role in abating the hazards of heavy metals.

  18. Anaerobic bioremediation of RDX by ovine whole rumen fluid and pure culture isolates.

    Science.gov (United States)

    Eaton, H L; Duringer, J M; Murty, L D; Craig, A M

    2013-04-01

    The ability of ruminal microbes to degrade the explosive compound hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) in ovine whole rumen fluid (WRF) and as 24 bacterial isolates was examined under anaerobic conditions. Compound degradation was monitored by high-performance liquid chromatography analysis, followed by liquid chromatography-tandem mass spectrometry identification of metabolites. Organisms in WRF microcosms degraded 180 μM RDX within 4 h. Nitroso-intermediates hexahydro-1-nitroso-3,5-dinitro-1,3,5-triazine (MNX), hexahydro-1,3-dinitroso-5-nitro-1,3,5-triazine (DNX), and hexahydro-1,3,5-trinitroso-1,3,5-triazine (TNX) were present as early as 0.25 h and were detected throughout the 24-h incubation period, representing one reductive pathway of ring cleavage. Following reduction to MNX, peaks consistent with m/z 193 and 174 were also produced, which were unstable and resulted in rapid ring cleavage to a common metabolite consistent with an m/z of 149. These represent two additional reductive pathways for RDX degradation in ovine WRF, which have not been previously reported. The 24 ruminal isolates degraded RDX with varying efficiencies (0-96 %) over 120 h. Of the most efficient degraders identified, Clostridium polysaccharolyticum and Desulfovibrio desulfuricans subsp. desulfuricans degraded RDX when medium was supplemented with both nitrogen and carbon, while Anaerovibrio lipolyticus, Prevotella ruminicola, and Streptococcus bovis IFO utilized RDX as a sole source of nitrogen. This study showed that organisms in whole rumen fluid, as well as several ruminal isolates, have the ability to degrade RDX in vitro and, for the first time, delineated the metabolic pathway for its biodegradation.

  19. Oxidation of acetate through reactions of the citric acid cycle by Geobacter sulfurreducens in pure culture and in syntrophic coculture.

    Science.gov (United States)

    Galushko, A S; Schink, B

    2000-11-01

    Geobacter sulfurreducens strain PCA oxidized acetate to CO2 via citric acid cycle reactions during growth with acetate plus fumarate in pure culture, and with acetate plus nitrate in coculture with Wolinella succinogenes. Acetate was activated by succinyl-CoA:acetate CoA-transferase and also via acetate kinase plus phosphotransacetylase. Citrate was formed by citrate synthase. Soluble isocitrate and malate dehydrogenases NADP+ and NAD+, respectively. Oxidation of 2-oxoglutarate was measured as benzyl viologen reduction and strictly CoA-dependent; a low activity was also observed with NADP+. Succinate dehydrogenase and fumarate ductase both were membrane-bound. Succinate oxidation was coupled to NADP+ reduction whereas fumarate reduction was coupled to NADPH and NADH Coupling of succinate oxidation to NADP+ or cytochrome(s) reduction required an ATP-dependent reversed electron transport. Net ATP synthesis proceeded exclusively through electron transport phosphorylation. During fumarate reduction, both NADPH and NADH delivered reducing equivalents into the electron transport chain, which contained a menaquinone. Overall, acetate oxidation with fumarate proceeded through an open loop of citric acid cycle reactions, excluding succinate dehydrogenase, with fumarate reductase as the key reaction for electron delivery, whereas acetate oxidation in the syntrophic coculture required the complete citric acid cycle.

  20. Susceptibility to antibiotics of Vibrio sp. AO1 growing in pure culture or in association with its hydroid host Aglaophenia octodonta (Cnidaria, Hydrozoa).

    Science.gov (United States)

    Stabili, Loredana; Gravili, Cinzia; Boero, Ferdinando; Tredici, Salvatore M; Alifano, Pietro

    2010-04-01

    Vibrio harveyi is the major causal organism of vibriosis, causing potential devastation to diverse ranges of marine invertebrates over a wide geographical area. These microorganisms, however, are phenotypically diverse, and many of the isolates are also resistant to multiple antibiotics. In a previous study, we described a previously unknown association between Vibrio sp. AO1, a luminous bacterium related to the species V. harveyi, and the benthic hydrozoan Aglaophenia octodonta. In this study, we analyzed the susceptibility to antibiotics (ampicillin, streptomycin, tetracycline, or co-trimoxazole = mix of sulfamethoxazole and trimetoprim) of Vibrio sp. AO1 growing in pure culture or in association with its hydroid host by using microcosm experiments. The results of minimum inhibitory concentration (MIC) experiments demonstrated that Vibrio sp. AO1 was highly resistant to ampicillin and streptomycin in pure culture. Nevertheless, these antibiotics, when used at sub-MIC values, significantly reduced the hydroid fluorescence. Co-trimoxazole showed the highest inhibitory effect on fluorescence of A. octodonta. However, in all treatments, the fluorescence was reduced after 48 h, but never disappeared completely around the folds along the hydrocaulus and at the base of the hydrothecae of A. octodonta when the antibiotic was used at concentration completely inhibiting growth in vitro. The apparent discrepancy between the MIC data and the fluorescence patterns may be due to either heterogeneity of the bacterial population in terms of antibiotic susceptibility or specific chemical-physical conditions of the hydroid microenvironment that may decrease the antibiotic susceptibility of the whole population. The latter hypothesis is supported by scanning electron microscope evidence for development of bacterial biofilm on the hydroid surface. On the basis of the results obtained, we infer that A. octodonta might behave as a reservoir of antibiotic multiresistant bacteria

  1. Study on pure IL VIV of a free spanning pipeline under general boundary conditions

    Science.gov (United States)

    Xu, Wan-hai; Xu, Jing-yu; Wu, Ying-xiang; Ji, Chun-ning

    2017-03-01

    Pipeline spans may occur due to natural seabed irregularities or local scour of bed sediment. The pure in-line (IL) vortex-induced vibrations (VIV) analysis of the free spans is an important subject for design of pipeline in uneven seabed. The main objective of this paper is to analyze the characteristics of pure IL VIV of a free spanning pipeline under general boundary conditions. An IL wake oscillator model which can describe the coupling of pipeline structure and fluctuating drag is introduced and employed. The coupled partial differential equations of structure and wake are transformed into a set of ordinary differential equations using two-mode Galerkin method. Some case studies are presented and thoroughly discussed in order to investigate the effects of internal fluid, axial force and boundary conditions on the pure IL VIV.

  2. Pure versus guided mirror exposure to reduce body dissatisfaction: a preliminary study with university women.

    Science.gov (United States)

    Moreno-Domínguez, Silvia; Rodríguez-Ruiz, Sonia; Fernández-Santaella, M Carmen; Jansen, Anita; Tuschen-Caffier, Brunna

    2012-03-01

    While effectiveness of mirror exposure to reduce body dissatisfaction has been demonstrated, the exposure was almost always combined with other interventions. The aim of the study was to evaluate the effectiveness of a pure mirror exposure intervention compared with a guided mirror exposure (participants are guided to describe their body shape in a non-evaluative manner) and an imagery exposure intervention (participants are guided to describe their body through mental representation). Thirty-one women with high body dissatisfaction received five sessions of treatment under one of the three conditions. All interventions reduced body dissatisfaction, but only the mirror exposures successfully reduced the frequency of negative thoughts and feelings of ugliness. Pure mirror exposure was more effective than guided exposure for reducing body discomfort within and between sessions. Pure mirror exposure, based on the traditional extinction paradigm, led to strong emotional activation followed by a fast decrease in emotional reactivity. Copyright © 2011 Elsevier Ltd. All rights reserved.

  3. Biodegradation potential of pure and mixed bacterial cultures for removal of 4-nitroaniline from textile dye wastewater.

    Science.gov (United States)

    Khalid, Azeem; Arshad, Muhammad; Crowley, David E

    2009-03-01

    Environmentally toxic aromatic amines including nitroanilines are commonly generated in dye contaminated wastewater in which azo dyes undergo degradation under anaerobic conditions. The aim of this study was to develop a process for biological treatment of 4-nitroaniline. Three bacteria identified as Acinetobacter sp., Citrobacter freundii and Klebsiella oxytoca were isolated from enrichment cultures of activated sludge on 4-nitroaniline, after which the isolates and the mixed culture were studied to determine optimal conditions for biodegradation. HPLC analyses showed the mixed culture was capable of complete removal of 100micromol/L of 4-nitroaniline within 72h under aerobic conditions. There was an inverse linear relationship (R(2)=0.96) between the rate of degradation (V) and 4-nitraoaniline concentrations [S] over 100-1000micromol/L. The bacterial culture was also capable of decolorizing structurally different azo dyes (Acid Red-88, Reactive Black-5, Direct Red-81, and Disperse Orange-3) and also degraded nitrobenzene. Our findings show that enrichment cultures from activated sludge can be effective for the removal of dyes and their toxic intermediates, and that treatment may best be accomplished using an anaerobic-aerobic process.

  4. Case study into the effect of intensive mass training on chronic pure alexia

    DEFF Research Database (Denmark)

    Wilms, Inge Linda

    2015-01-01

    This paper describes the results from a case study into the effects of intensive reading training on a patient with acquired pure alexia disorder. The training targeted the various stages of the basic reading process based on the theories of the psycholinguistic language model as a mixture of bot...

  5. Plant Tissue Culture Studies.

    Science.gov (United States)

    Smith, Robert Alan

    Plant tissue culture has developed into a valid botanical discipline and is considered a key area of biotechnology, but it has not been a key component of the science curriculum because of the expensive and technical nature of research in this area. This manual presents a number of activities that are relatively easy to prepare and perform. The…

  6. Exploring characteristics of bioelectricity generation and dye decolorization of mixed and pure bacterial cultures from wine-bearing wastewater treatment.

    Science.gov (United States)

    Han, Jing-Long; Liu, Ying; Chang, Chang-Tang; Chen, Bor-Yann; Chen, Wen-Ming; Xu, Hui-Zhong

    2011-04-01

    This study uncovered microbial characteristics of bioelectricity generation and dye decolorization in single-chamber microbial fuel cells (MFCs) using activated sludge for wine-containing wastewater treatment. Phylogenetic tree analysis on 16S rRNA gene fragments indicated that the predominant strains on anodic biofilm in acclimatized MFCs were Gamma-Proteobacteria Aeromonas punctata NIU-P9, Pseudomonas plecoglossicida NIU-Y3, Pseudomonas koreensis NIU-X8, Acinetobacter junii NIU-Y8, Stenotrophomonas maltophila NIU-X2. Our findings showed that the current production capabilities of these pure strains were only ca. 10% of those of their mother activated sludge, indicating that synergistic interactions among microbes might be the most influential factor to maximize power generation in MFCs. Plus, these electrochemically active strains also performed reductive decolorization of C.I. reactive blue 160, suggesting that bioelectricity generation might be directly associated to azo dye decolorization to deal with electron transfer on anodic biofilm in MFCs.

  7. Studies on optical properties of pure and Mg2+ doped ZTS single crystal

    Science.gov (United States)

    Sumithra Devi, M.; Arthi, A. P.; Thamizharasan, K.

    2014-09-01

    Single crystal of semiorganic nonlinear optical material of pure and Mg2+ doped ZTS were grown by slow evaporation technique from its aqueous solution. The grown crystal was characterized by single crystal XRD to determine the cell parameters. The Mg2+ doped was characterized by SEM and EDX spectrum. The chemical composition of both the pure ZTS and doped Mg2+ crystals were determined by Fourier transform infrared (FTIR). The optical absorption spectrum recorded in the wavelength was ranged from 200 to 2500 nm and its energy gap (Eg) for both pure ZTS is 4.47 eV and doped Mg2+ is 4.42 eV. The mechanical strength of the grown crystal was found from Vicker's microhardness measurements. It showed that pure and doped ZTS crystals are soft in nature. The dielectric measurement was carried out to study the different polarization mechanism and conductivity of the crystal. Photoconductivity studies revealed that the positive photoconductivity of the both were considered as grown crystals.

  8. ERD studies of D-ion depth distributions after implantation into some pure metals and alloys

    Science.gov (United States)

    Didyk, A. Yu.; Wiśniewski, R.; Wilczynska, T.; Kitowski, K.; Hofman, A.

    2012-01-01

    This paper presents a report on experimental results of depth distributions of deuterium ions implanted with 25 keV energy at a fluence interval of (1.2-2.3) × 1022 m-2 into samples of pure metals (Cu, Ti, Zr, V, Pd) and diluted Pd alloys (Pd-Ag, Pd-Pt, Pd-Ru, Pd-Rh). The post-treatment depth distributions of deuterium and hydrogen atoms were measured within a few hours after implantation with the use of elastic recoil detection (ERD) analysis. After three months the measurements were repeated. The comparison of the obtained results in both series of studies allowed us to make an important observation of the desorption rates of implanted deuterium atoms from pure metals and diluted Pd alloys. The maximum measured concentrations of deuterium atoms in pure Zr and Ti foils with relatively small desorption rate of deuterium atoms within three months after implantation were observed. Also a very high spreading of deuterium atom distributions was observed in all the measured pure metals and alloys. It can be explained by the large diffusion coefficients of deuterium and extremely fast kinetics.

  9. Kinetics and mechanical study of plasma electrolytic carburizing for pure iron

    Science.gov (United States)

    Çavuşlu, F.; Usta, M.

    2011-02-01

    In this work, plasma electrolytic surface carburizing of pure iron in aqueous solution consisting of water, glycerin and NH4Cl was investigated. Surface carburizing was carried out in 20% glycerin solution treated at 750 °C, 800 °C, 900 °C and 950 °C temperatures for 5, 10 and 30 min. The formation of hard carbon-rich layer on the surface of pure iron was confirmed by XRD analysis. Metallographic and SEM studies revealed a rough and dense carburized layer on the surface of the pure iron. Experimental results showed that the thickness of the carburized layers changes with the time and temperature. The average thickness of the carburized layer ranged from 20 to 160 μm. The hardness of the carburized samples decreased with the distance from the surface to the interior of the test material. The average hardness values of the carburized layers on the substrate ranged 550-850 HV, while the hardness of the substrate ranged from 110 HV to 170 HV. The dominant phases formed on the pure iron were found to be a mixture of cementite (Fe3C), martensite (Fe + C) and austenite (FCC iron) confirmed by XRD. Wear resistance in all plasma electrolytic carburized samples is considerably improved in relation to the untreated specimen. After carburizing, surface roughness of the samples was increased. Friction coefficients were also increased because of high surface roughness.

  10. Dynamic model-based analysis of furfural and HMF detoxification by pure and mixed batch cultures of S. cerevisiae and S. stipitis.

    Science.gov (United States)

    Hanly, Timothy J; Henson, Michael A

    2014-02-01

    Inhibitory compounds that result from biomass hydrolysis are an obstacle to the efficient production of second-generation biofuels. Fermentative microorganisms can reduce compounds such as furfural and 5-hydroxymethyl furfural (HMF), but detoxification is accompanied by reduced growth rates and ethanol yields. In this study, we assess the effects of these furan aldehydes on pure and mixed yeast cultures consisting of a respiratory deficient mutant of Saccharomyces cerevisiae and wild-type Scheffersomyces stipitis using dynamic flux balance analysis. Uptake kinetics and stoichiometric equations for the intracellular reduction reactions associated with each inhibitor were added to genome-scale metabolic reconstructions of the two yeasts. Further modification of the S. cerevisiae metabolic network was necessary to satisfactorily predict the amount of acetate synthesized during HMF reduction. Inhibitory terms that captured the adverse effects of the furan aldehydes and their corresponding alcohols on cell growth and ethanol production were added to attain qualitative agreement with batch experiments conducted for model development and validation. When the two yeasts were co-cultured in the presence of the furan aldehydes, inoculums that reduced the synthesis of highly toxic acetate produced by S. cerevisiae yielded the highest ethanol productivities. The model described here can be used to generate optimal fermentation strategies for the simultaneous detoxification and fermentation of lignocellulosic hydrolysates by S. cerevisiae and/or S. stipitis.

  11. Pure waterjet drilling of articular bone: an in vitro feasibility study:

    OpenAIRE

    Biskup, Christian; Dunnen, Steven den; Kerkhoffs, Gino M. M. J.; Kraaij, Gert; Tuijthof, Gabrielle J. M.

    2013-01-01

    The clinical application of waterjet technology for machining tough human tissues, such as articular bone, has advantages, as it produces clean sharp cuts without tissue heating. Additionally, water supply is possible via flexible tubing, which enables minimally invasive surgical access. This pilot study investigates whether drilling bony tissue with pure waterjets is feasible. Water pressures between 20 and 120 MPa with an orifice of 0.6 mm were used to create waterjets to drill blind boring...

  12. Studies on structural and electrical properties of pure and doped h-YMnO3

    Science.gov (United States)

    Rathod, K. N.; Rajyaguru, Bhargav; Solanki, Sapana; Shrimali, V. G.; Sagapariya, Khushal; Markna, J. H.; Solanki, P. S.; Shah, N. A.

    2016-05-01

    In the present communication, we report the results of the structural and electrical studies on pure YMnO3 (YMO) and Zr doped Y0.95Zr0.05MnO3 (YZMO) samples, synthesized using low cost sol-gel technique. To understand the structural properties, structural phases present and effect of doping on the structural behavior, X-ray diffraction (XRD) measurements were carried out for both the samples understudy. XRD results reveal the single phasic nature of both the samples having hexagonal unit cell structure. Improved crystal structure has been observed for pure YMnO3 sample while doping of Zr at Y-site degrades the structure of the sample. Improved dielectric constant and enhanced AC conductivity (σa) have been discussed on the basis of charge carrier polarization and correlated barrier hopping due to the localized state.

  13. Study on A.C. electrical properties of pure and L-serine doped ADP crystals

    Science.gov (United States)

    Joshi, J. H.; Dixit, K. P.; Joshi, M. J.; Parikh, K. D.

    2016-05-01

    Ammonium Dihydrogen Phosphate (ADP) crystals have a wide range of applications in integrated and nonlinear optics. Amino acids having significant properties like molecular chirality, zwitter ionic nature, etc. attracted many researchers to dope them in various NLO crystals. In the present study, pure and different weight percentage L-serine doped ADP crystals were grown by slow solvent evaporation technique at room temperature. The A.C. electrical study was carried out for palletized samples at room temperature. The Nyquist plot showed two semi circles for pure ADP indicated the effect of grain and grain boundary, whereas the doped ADP samples exhibited the single semi circle suggesting the effect of grain. The values resistance and capacitance for grain and grain boundary were calculated. The effect of doping was clearly seen in the grain capacitance and resistance values. The dielectric constant and dielectric loss decreased with increase in frequency for all samples. The Jonscher power law was applied for A.C. conductivity for pure and doped ADP samples. The imaginary part of modulus and impedance versus frequency were drawn and the value of stretch exponent (β) was calculated for all the samples.

  14. A Comparative Study of the Sintering Behavior of Pure and Manganese-Substituted Hydroxyapatite

    Directory of Open Access Journals (Sweden)

    Michael Zilm

    2015-09-01

    Full Text Available Hydroxyapatite (HA is a widely studied biomaterial for its similar chemical composition to bone and its osteoconductive properties. The crystal structure of HA is flexible, allowing for a wide range of substitutions which can alter bioactivity, biodegradation, and mechanical properties of the substituted apatite. The thermal stability of a substituted apatite is an indication of its biodegradation in vivo. In this study, we investigated the thermal stability and mechanical properties of manganese-substituted hydroxyapatite (MnHA as it is reported that manganese can enhance cell attachment compared to pure HA. Pure HA and MnHA pellets were sintered over the following temperature ranges: 900 to 1300 °C and 700 to 1300 °C respectively. The sintered pellets were characterized via density measurements, mechanical testing, X-ray diffraction, and field emission electron microscopy. It was found that MnHA was less stable than HA decomposing around 800 °C compared to 1200 °C for HA. The flexural strength of MnHA was weaker than HA due to the decomposition of MnHA at a significantly lower temperature of 800 °C compared to 1100 °C for HA. The low thermal stability of MnHA suggests that a faster in vivo dissolution rate compared to pure HA is expected.

  15. MicroPure imaging for the evaluation of microcalcifications in gouty arthritis involving the first metatarsophalangeal joint: a preliminary study.

    Directory of Open Access Journals (Sweden)

    Lu Yin

    Full Text Available OBJECTIVE: To assess the value of MicroPure, a new ultrasound image processing technique, in identifying microcalcifications (formed by monosodium urate crystals in the first metatarsophalangeal joints attacked by gout compared to gray-scale ultrasound images. MATERIALS AND METHODS: Thirty-six patients who fulfilled the study inclusion criteria underwent gray-scale ultrasound and MicroPure examinations of the first metatarsophalangeal joints attacked by gout. Static images of the target areas were acquired using gray-scale ultrasound and MicroPure. Two independent and blinded investigators analyzed the images to determine the number of microcalcifications and to score for image quality and artifacts. RESULTS: The two investigators observed significantly more microcalcifications with MicroPure compared to gray-scale ultrasound (ρ<0.001. The level of agreement between the investigators consistently increased from gray-scale ultrasound to MicroPure imaging (gray-scale interclass correlation coefficient of 0.69 vs. MicroPure interclass correlation coefficient of 0.81. One investigator preferred the MicroPure image quality over gray-scale ultrasound (ρ<0.001, but the other investigator disagreed (ρ<0.001. Both investigators observed fewer artifacts with MicroPure than with gray-scale ultrasound (ρ<0.009. CONCLUSION: MicroPure imaging identified significantly more microcalcifications than gray-scale ultrasound.

  16. Hydration of pure and base-Containing sulfuric acid clusters studied by computational chemistry methods

    Science.gov (United States)

    Henschel, Henning; Ortega, Ismael K.; Kupiainen, Oona; Olenius, Tinja; Kurtén, Theo; Vehkamäki, Hanna

    2013-05-01

    The formation of hydrates of small molecular sulfuric acid clusters and cluster containing both sulfuric acid and base (ammonia or dimethylamine) has been studied by means of computational chemistry. Using a combined ab initio/density functional approach, formation energies of clusters with up to four sulfuric acid molecules, and up to two base molecules, have been calculated. Consequences for the hydration level of the corresponding clusters have been modelled. While the majority of pure sulfuric acid cluster are comparatively strongly hydrated, base containing cluster were found to be less hydrophilic. Dimethylamine is particularly effective in lowering the hydrophilicity of the cluster. Implications of the hydration profiles on atmospheric processes are discussed.

  17. Comparative genomic analysis of the gut bacterium Bifidobacterium longum reveals loci susceptible to deletion during pure culture growth

    Directory of Open Access Journals (Sweden)

    Shakhova VV

    2008-05-01

    Full Text Available Abstract Background Bifidobacteria are frequently proposed to be associated with good intestinal health primarily because of their overriding dominance in the feces of breast fed infants. However, clinical feeding studies with exogenous bifidobacteria show they don't remain in the intestine, suggesting they may lose competitive fitness when grown outside the gut. Results To further the understanding of genetic attenuation that may be occurring in bifidobacteria cultures, we obtained the complete genome sequence of an intestinal isolate, Bifidobacterium longum DJO10A that was minimally cultured in the laboratory, and compared it to that of a culture collection strain, B. longum NCC2705. This comparison revealed colinear genomes that exhibited high sequence identity, except for the presence of 17 unique DNA regions in strain DJO10A and six in strain NCC2705. While the majority of these unique regions encoded proteins of diverse function, eight from the DJO10A genome and one from NCC2705, encoded gene clusters predicted to be involved in diverse traits pertinent to the human intestinal environment, specifically oligosaccharide and polyol utilization, arsenic resistance and lantibiotic production. Seven of these unique regions were suggested by a base deviation index analysis to have been precisely deleted from strain NCC2705 and this is substantiated by a DNA remnant from within one of the regions still remaining in the genome of NCC2705 at the same locus. This targeted loss of genomic regions was experimentally validated when growth of the intestinal B. longum in the laboratory for 1,000 generations resulted in two large deletions, one in a lantibiotic encoding region, analogous to a predicted deletion event for NCC2705. A simulated fecal growth study showed a significant reduced competitive ability of this deletion strain against Clostridium difficile and E. coli. The deleted region was between two IS30 elements which were experimentally

  18. Thiocyanate degradation by pure and mixed cultures of microorganisms Degradação de tiocianato por culturas puras e mixtas

    Directory of Open Access Journals (Sweden)

    Elaine M. Souza-Fagundes

    2004-12-01

    Full Text Available A mixed culture and a pure bacterial strain (BMV8 were isolated from a bioreactor for thiocyanate treatment. Both cultures removed 5 mM of thiocyanate from the medium in 36 hours. The mixed culture was able to tolerate concentrations up to 60 mM. The efficiency of thiocyanate degradation decreased when the cells were immobilized.Uma cultura mixta e uma linhagem bacteriana pura foram isoladas de um bioreator para tratamento de tiocianato. As culturas removeram 5mM de tiocianato do meio em 36 horas. A cultura mixta foi capaz de tolerar concentrações superiores a 60mM. A eficiência da degradação de tiocianato diminuiu quando as células foram imobilizadas.

  19. Systematic experimental study of pure shear type dielectric elastomer membranes with different electrode and film thicknesses

    Science.gov (United States)

    Hodgins, M.; Seelecke, S.

    2016-09-01

    An approach to reduce the voltage required for dielectric elastomer actuators is to reduce film thickness. However, if the electrode thickness is not similarly reduced, the electrode’s mechanical behavior can increasingly and negatively impact the overall actuator behavior. This effect is yet to be studied and quantified for pure shear type specimens; a type recommended in a recent DE standardization journal publication. Therefore, in this work, using pure shear specimens, a comparative study of membrane actuators of different film thickness (20, 50 and 100 μm) is performed. Electrodes of different thicknesses are screen printed and tested in a uniaxial test device. The stiffening effect due to the solid-state electrodes is demonstrated by performing force-elongation tests for specimens with and without electrodes. Additionally the importance of thin electrodes (relative to film thickness) was demonstrated through a number of electromechanical tests. Isotonic tests revealed a lower electro-mechanical sensitivity for the 20 μm film when compared with the 50 and 100 μm films. This was attributed to the relatively thick electrodes. Best actuation results were achieved when the total electrode thickness was at least 15x thinner than the dielectric membrane thickness.

  20. Theoretical Study of Renewable Ionic Liquids in the Pure State and with Graphene and Carbon Nanotubes.

    Science.gov (United States)

    García, Gregorio; Atilhan, Mert; Aparicio, Santiago

    2015-09-17

    The N-ethyl-N-(furan-2-ylmethyl)ethanaminium dihydrogen phosphate ionic liquid was studied as a model of ionic liquids which can be produced from totally renewable sources. A computational study using both molecular dynamics and density functional theory methods was carried out. The properties, structuring, and intermolecular interactions (hydrogen bonding) of this fluid in the pure state were studied as a function of pressure and temperature. Likewise, the adsorption on graphene and the confinement between graphene sheets was also studied. The solvation of single walled carbon nanotubes in the selected ionic liquid was analyzed together with the behavior of ions confined inside these nanotubes. The reported results show remarkable properties for this fluid, which show that many of the most relevant properties of ionic liquids and their ability to interact with carbon nanosystems may be maintained and even improved using new families of renewable compounds instead of classic types of ionic liquids with worse environmental, toxicological, and economical profiles.

  1. Comparative study of biological hydrogen production by pure strains and consortia of facultative and strict anaerobic bacteria.

    Science.gov (United States)

    Hiligsmann, Serge; Masset, Julien; Hamilton, Christopher; Beckers, Laurent; Thonart, Philippe

    2011-02-01

    In this paper, a simple and rapid method was developed in order to assess in comparative tests the production of binary biogas mixtures containing CO(2) and another gaseous compound such as hydrogen or methane. This method was validated and experimented for the characterisation of the biochemical hydrogen potential of different pure strains and mixed cultures of hydrogen-producing bacteria (HPB) growing on glucose. The experimental results compared the hydrogen production yield of 19 different pure strains and sludges: facultative and strict anaerobic HPB strains along with anaerobic digester sludges thermally pre-treated or not. Significant yields variations were recorded even between different strains of the same species by i.e. about 20% for three Clostridium butyricum strains. The pure Clostridium butyricum and pasteurianum strains achieved the highest yields i.e. up to 1.36 mol H(2)/mol glucose compared to the yields achieved by the sludges and the tested Escherichia and Citrobacter strains.

  2. Cultural Language Study: Grade 7.

    Science.gov (United States)

    Schwartz, Betty L.; Tappenden, Jacqueline W.

    This course guide, the first in a two-year sequence, is designed to give students an overview of Greek and Roman culture and language from the era of the early Aegean civilizations in Greece and Asia Minor to the Augustan Age in Rome. Six units of study are concerned with the growth and development of Greece and with the metamorphosis of Rome from…

  3. Anthropological Studies of Popular Culture

    Directory of Open Access Journals (Sweden)

    Bojan Žikić

    2016-02-01

    Full Text Available One of the questions raised at the symposium "Our World, Other Worlds. Anthropology, Science Fiction and Cultural Identity", held in Belgrade in December 2009, is how anthropology is to study contemporary art forms: how research issues are to be defined and approached; how research is to be organized in a specific semantic area, which cannot always and with absolute certainty be said not to be an anthropological construction; whether the subject of research can be said to have the shared nature of cultural communication; whether the anthropologist is to interpret the author/artist’s intention, or that which is produced as a result of that intention, etc. The aim of this paper is to suggest some answers to these questions, from the point of view of a researcher focused on cultural communication.

  4. Study on Productivity of some Legume Crops in Pure Cultivation and Mixtures

    Directory of Open Access Journals (Sweden)

    Viliana Vasileva

    2014-02-01

    Full Text Available In the conditions of pot trial the productivity of birdsfoot trefoil (Lotus corniculatus L. cv. ‘Targovishte’ 1”; sainfoin (Onobrychis Adans. breeding N26, white clover (Тrifolium repens L. cv. ‘Milka’, and subclover (Trifolim subterraneum ssp. brachycalicinum cv. ‘Clear’, in pure cultivation and in mixtures was studied. The treatments were as follows: 1. birdsfoot trefoil 100%; 2. sainfoin 100%; 3. white clover 100%; 4. subclover 100%; 5. cocksfoot 100%; 6. birdsfoot trefoil+cocksfoot; 7. sainfoin+cocksfoot 8. white clover+cocksfoot; 9. subclover+cocksfoot; 10. birdsfoot trefoil+ subclover; 11. sainfoin+subclover; 12. white clover+subclover. The ratio between components in the mixtures was 50:50. Cocksfoot (Dactylis glomerata L. cv. ‘Dabrava’ as a grass component was used. It was found, that aboveground dry matter yield of white clover, birdsfoot trefoil, sainfoin and subclover in mixture with cocksfoot was higher as compared to these in pure cultivation. Subclover in mixture with birdsfoot trefoil increased aboveground dry matter yield, but in mixture with sainfoin and white clover did not contribute to increase of their productivity.

  5. Preparation and mechanism study of bulk pure rare-earth metals with amorphous and nanocrystalline structures

    Institute of Scientific and Technical Information of China (English)

    LI ErDong; SONG XiaoYan; ZHANG JiuXing; LU NianDuan

    2007-01-01

    The preparation and the mechanism study of bulk pure rare-earth metals with amorphous and nanocrystalline structures, which were produced by spark plasma sintering (SPS), were carried out in this paper. With different processing parameters, the amorphous, two phases of amorphous and nanocrystalline, and complete nanocrystalline microstructures have been obtained. The nano-grain sizes in the bulk nanocrystalline materials are found smaller than the original powder particles sizes, which may change the conventional viewpoint that the grains in the sintered bulk are generally coarser than the raw powder particles. The technique developed in the present work can be extended to the preparation of many other nano bulk metal materials, and thus enables the studies of the nano-size effects on the physical, chemical and mechanical properties of bulk nano materials.

  6. Determinants of lexical access in pure-anomic recovery:a longitudinal study

    Institute of Scientific and Technical Information of China (English)

    Xiao ZHOU; Hui LIANG; Ming-wei XU; Ben-yan LUO

    2009-01-01

    Many studies involving lexical access in picture-naming tasks have been undertaken at a point in time, mainly focusing on age of acquisition (AoA). To identify the real determinates of lexical access in recovery and their traces in the brain, we carried out a longitudinal study on a Chinese pure anomic patient using multiple logistic regression analysis. We found that AoA played an important role in early recovery but not in total recovery, whereas familiarity was significant in the whole process. From a new dynamic point of view, our results indicate that AoA and familiarity are the main determinants of lexical access in anomia recovery. We suggest that the changing effects of AoA during recovery may be related to the pathologic process; AoA and familiarity should be taken into account in constructing materials to assess and treat anomic patients.

  7. Effects of pure starter cultures on physico-chemical and sensory quality of dry fermented Chinese-style sausage.

    Science.gov (United States)

    Rai, Krishna P; Zhang, Chunhui; Xia, Wen Shui

    2010-03-01

    Dry fermented Chinese-style sausages prepared in laboratory inoculating with Lactobacillus casei subsp. casei-1.001, Pediococcus pentosaceus-ATCC 33316, Staphylococcus xylosus-12 and without starter culture randomly sampled at 0, 3, 10, and 24 days of ripening were analyzed for physico-chemical and sensory qualities. A significant (psausage during ripening was observed, whereas other major chemical parameters remained unchanged. The microbial fermentation resulted in decreased pH and nitrite but increased non protein nitrogen and total volatile basic nitrogen in the products. Starter cultures except P. Pentosaceous-ATCC 33316, used in the sausage failed to suppress rancidity in ripened product as indicated by a significant (psausages decreased with ripening time, meanwhile the redness (a) increased significantly (psausages inoculated with cultures L. casei subsp. casei-1.001 and S. xylosus-12. The texture profile of sausages was almost similar except for P. Pentosaceous-ATCC 33316, which showed significantly (psausage of high quality.

  8. Structural and photocatalytic studies on pure and Sn ion doped ZnO-graphene nanocomposites

    Science.gov (United States)

    Beura, Rosalin; Thangadurai, P.

    2016-05-01

    Graphene based metal oxide nanocomposites have been widely used as a photocatalyst for the treatment of water pollutants. This work demonstrates the synthesis of graphene composite with pure and Sn ion doped-ZnO and their photocatalytic properties are reported. Structural studies were carried out by X-ray diffraction and Raman spectroscopy to confirm the formation of the nanocomposites. Microstructure was characterized by scanning electron microscopy showing rod shaped ZnO and the layer structured graphene in the ZnO-graphene composite. In comparison with the undoped ZnO-graphene composite, the Sn ion doped ZnO-graphene composite have shown better degradation of methyl orange dye that is about 99% of degradation. Band gap of the composite materials was calculated to be 3.36 eV from the UV-Vis result.

  9. Production of NO and N2O by Pure Cultures of Nitrifying and Denitrifying Bacteria during Changes in Aeration

    NARCIS (Netherlands)

    Kester, R.A.; Boer, W. de; Laanbroek, H.J.

    1997-01-01

    Peak emissions of NO and N2O are often observed after wetting of soil. The reactions to sudden changes in the aeration of cultures of nitrifying and denitrifying bacteria with respect to NO and N2O emissions were compared to obtain more information about the microbiological aspects of peak emissions

  10. Brain mechanisms of recovery from pure alexia: A single case study with multiple longitudinal scans.

    Science.gov (United States)

    Cohen, Laurent; Dehaene, Stanislas; McCormick, Samantha; Durant, Szonya; Zanker, Johannes M

    2016-10-01

    Pure alexia is an acquired reading disorder, typically due to a left occipito-temporal lesion affecting the Visual Word Form Area (VWFA). It is unclear whether the VWFA acts as a unique bottleneck for reading, or whether alternative routes are available for recovery. Here, we address this issue through the single-case longitudinal study of a neuroscientist who experienced pure alexia and participated in 17 behavioral, 9 anatomical, and 9 fMRI assessment sessions over a period of two years. The origin of the impairment was assigned to a small left fusiform lesion, accompanied by a loss of VWFA responsivity and by the degeneracy of the associated white matter pathways. fMRI experiments allowed us to image longitudinally the visual perception of words, as compared to other classes of stimuli, as well as the mechanisms of letter-by-letter reading. The progressive improvement of reading was not associated with the re-emergence of a new area selective to words, but with increasing responses in spared occipital cortex posterior to the lesion and in contralateral right occipital cortex. Those regions showed a non-specific increase of activations over time and an increase in functional correlation with distant language areas. Those results confirm the existence of an alternative occipital route for reading, bypassing the VWFA, but they also point to its key limitation: the patient remained a slow letter-by-letter reader, thus supporting the critical importance of the VWFA for the efficient parallel recognition of written words. Copyright © 2016 Elsevier Ltd. All rights reserved.

  11. Comparison of Outcomes between Individuals with Pure and Mixed Lupus Nephritis: A Retrospective Study.

    Directory of Open Access Journals (Sweden)

    Titilayo O Ilori

    Full Text Available Lupus nephritis (LN is a serious organ manifestation of systemic lupus erythematosus. Histologic overlap is relatively common in the six pathologic classes (I to VI of LN. For example, mixed proliferative LN (MPLN often includes features of classes III & V or classes IV & V combined. We performed a comparative evaluation of renal outcomes in patients with MPLN to patients with pure proliferative LN (PPLN against pre-specified renal outcomes, and we also identified predictor of clinical outcomes among those with PPLN and MPLN.Individuals with MPLN will have worse short-term renal outcomes compared to those with PPLN.We retrospectively reviewed 278 adult LN patients (≥18 years old identified from an Emory University Hospital registry of native renal biopsies performed between January 2000 and December 2011. The final analytic sample consisted of individuals with a diagnosis of PPLN (n = 60 and MPLN (n = 96. We analyzed differences in clinical and laboratory characteristics at baseline. We also assessed associations between LN category and renal outcomes (complete remission and time to ESRD with logistic and Cox proportional hazards models within two years of baseline.The study population was predominantly female (83.97% and African American (71.8% with a mean age of 33.4 years at baseline. Over a median follow up of 1.02 years, we did not find any statistically significant associations between MPLN and the development of ESRD or remission when compared to patients with PPLN (adjusted HR = 0.30, 95% CI = 0.07, 1.26.There was no association between mixed or pure histopathologic features of LN at presentation and rate of complete or partial remission but higher baseline eGFR was associated with a lower probability of complete remission among patients with lupus nephritis.

  12. In vitro studies on silver implanted pure iron by metal vapor vacuum arc technique.

    Science.gov (United States)

    Huang, Tao; Cheng, Yan; Zheng, Yufeng

    2016-06-01

    Pure iron has been verified as a promising biodegradable metal for absorbable cardiovascular stent usage. However, the degradation rate of pure iron is too slow. To accelerate the degradation of the surface of pure iron, silver ions were implanted into pure iron by metal vapor vacuum arc (MEVVA) source at an extracted voltage of 40keV. The implanted influence was up to 2×10(17)ions/cm(2). The composition and depth profiles, corrosion behavior and biocompatibility of Ag ion implanted pure iron were investigated. The implantation depths of Ag was around 60nm. The element Ag existed as Ag2O in the outermost layer, then gradually transited to metal atoms in zero valent state with depth increase. The implantation of Ag ions accelerated the corrosion rate of pure iron matrix, and exhibited much more uniform corrosion behavior. For cytotoxicity assessment, the implantation of Ag ions slightly decreased the viability of all kinds of cell lines used in these tests. The hemolysis rate of Ag ion implanted pure iron was lower than 2%, which was acceptable, whereas the platelet adhesion tests indicated the implantation of Ag ions might increase the risk of thrombosis.

  13. Decay studies and mass measurements on isobarically pure neutron-rich Hg and Tl isotopes

    CERN Multimedia

    Schweikhard, L C; Savreux, R P; Hager, U D K; Beck, D; Blaum, K

    2007-01-01

    We propose to perform mass measurements followed by $\\beta$- and $\\gamma$-decay studies on isobarically pure beams of neutron-rich Hg and Tl isotopes, which are very poorly known due to a large contamination at ISOL-facilities with surface-ionised francium. The aim is to study the binding energies of mother Hg and Tl nuclides, as well as the energies, spins and parities of the excited and ground states in the daughter Tl and Pb isotopes. The proposed studies will address a new subsection of the nuclear chart, with Z 126, where only 9 nuclides have been observed so far. Our studies will provide valuable input for mass models and shell-model calculations: they will probe the proton hole-neutron interaction and will allow to refine the matrix elements for the two-body residual interaction. Furthermore, they also give prospects for discovering new isomeric states or even new isotopes, for which the half-lives are predicted in the minute- and second-range.\\\\ To reach the isobaric purity, the experiments will be p...

  14. Study of a condition for the mode conversion from purely perpendicular electrostatic waves to electromagnetic waves

    Science.gov (United States)

    Kalaee, Mohammad Javad; Katoh, Yuto

    2016-07-01

    One of the mechanisms for generating electromagnetic plasma waves (Z-mode and LO-mode) is mode conversion from electrostatic waves into electromagnetic waves in inhomogeneous plasma. Herein, we study a condition required for mode conversion of electrostatic waves propagating purely perpendicular to the ambient magnetic field, by numerically solving the full dispersion relation. An approximate model is derived describing the coupling between electrostatic waves (hot plasma Bernstein mode) and Z-mode waves at the upper hybrid frequency. The model is used to study conditions required for mode conversion from electrostatic waves (electrostatic electron cyclotron harmonic waves, including Bernstein mode) into electromagnetic plasma waves (LO-mode). It is shown that for mode conversion to occur in inhomogeneous plasma, the angle between the boundary surface and the magnetic field vector should be within a specific range. The range of the angle depends on the norm of the k vector of waves at the site of mode conversion in the inhomogeneous region. The present study reveals that inhomogeneity alone is not a sufficient condition for mode conversion from electrostatic waves to electromagnetic plasma waves and that the angle between the magnetic field and the density gradient plays an important role in the conversion process.

  15. An Eye Movement Study on the Role of the Visual Field Defect in Pure Alexia

    Science.gov (United States)

    Bormann, Tobias; Wolfer, Sascha A.; Hachmann, Wibke; Lagrèze, Wolf A.; Konieczny, Lars

    2014-01-01

    Pure alexia is a severe impairment of word reading which is usually accompanied by a right-sided visual field defect. Patients with pure alexia exhibit better preserved writing and a considerable word length effect, claimed to result from a serial letter processing strategy. Two experiments compared the eye movements of four patients with pure alexia to controls with simulated visual field defects (sVFD) when reading single words. Besides differences in response times and differential effects of word length on word reading in both groups, fixation durations and the occurrence of a serial, letter-by-letter fixation strategy were investigated. The analyses revealed quantitative and qualitative differences between pure alexic patients and unimpaired individuals reading with sVFD. The patients with pure alexia read words slower and exhibited more fixations. The serial, letter-by-letter fixation strategy was observed only in the patients but not in the controls with sVFD. It is argued that the VFD does not cause pure alexic reading. PMID:24999811

  16. The Pure Culture of Fruiting Bodies of Scorias spongiosa,an Edible Mushroom of Bamboo%竹类食用蕈菌——海绵胶煤炱菌子实体的纯培养

    Institute of Scientific and Technical Information of China (English)

    贺新生; 刘超洋; 郑俊娟; 商圆圆; 霍存录

    2012-01-01

    The study explored the pure culture condition and development law of the fruiting bodies of Scorias spongiosa, in order to provide technical solutions for large - scale production of this new type of mushroom. More than 100 specimen of wild fruitbodies were collected from Zhejiang, Sichuan and Jiangsu province. Pure strains were obtained through tissus separation and pure culturing on agar, liquid and solid medium. Mycelium directly secreted gelatinous material to form colloidal fruitbodies in the agar and solid culture medium. In liquid medium, only mycelium and the mycelium sphere were formed, but not fruitbodies. The development process of this fungus was: conidia spore germination→ hyphal→colloid tissue→ branches of fruitbodies → small fruitbodies → formation pycnidium and spores liquid group → releasing spores. Under pure culture conditions it was difficult to form large fruiting bodies.%探索海绵胶煤炱菌子实体形成的培养条件,研究该菌的子实体发育规律,为规模化生产这种新型食用蕈菌提供技术方案.在浙江、四川、江苏等地共采集到100多份野生子实体标本,经组织分离得到纯培养菌株,在多种琼脂、液体、固体培养基上进行纯培养.在琼脂和固体培养基上,菌丝体直接分泌胶质物质形成胶质的小型子实体;在液体培养基上只形成菌丝和胶质菌丝球,不形成子实体.该菌的形态发育过程:分生孢子萌发→菌丝→胶质组织→子实体分枝→小型子实体→形成分生孢子器和分生孢子→释放孢子.海绵胶煤炱菌在纯培养条件下难以形成大型子实体.

  17. Determination of Cyproheptadine hydrochloride in Pure and Pharmaceutical forms : A Spectrophotometric study

    Directory of Open Access Journals (Sweden)

    Sayanna

    2015-09-01

    Full Text Available Siimple and sensitive extractive spectrophotometric methods have been described for the determination of Cyproheptadine hydrochloride (CPH in pure form and in pharmaceutical formulations. The developed methods involved formation of coloured chloroform extractable ion-pair complexes of the CPH with triphenyl methane dyes viz., bromothymol blue (BTB, bromocresol green (BCG and bromocresol purple (BCP in acidic medium. The extracted complexes showed absorbance maxima at 419, 419 and 405nm with use of the above dyes, respectively. Beer’s law is obeyed in the concentration ranges 3.0-25, 2.5-22.5 and 1.5-25μg/ml with BTB, BCG and BCP respectively. The stoichiometry of the complex is found to be 1:1 in each case. The effect of concentration of dye, pH and interference of excipients have been studied and optimized. The limit of detection and limit of quantification have been determined for three methods. The methods are applied to the determination of CPH in commercial tablets and results of analysis are validated statistically through recovery studies also the methods are validated as per the guidelines of ICH.

  18. 纯菌种培养红茶菌中细菌纤维素的合成%Bacterial cellulose in Kombucha by pure cultures

    Institute of Scientific and Technical Information of China (English)

    周艳; 谭丽丽; 唐欣昀

    2012-01-01

    实验采用纯菌种培养红茶菌研究细菌纤维素(bacterial cellulose,BC)的合成量及产率,所用的菌株为汉逊氏葡糖酸醋杆菌(Gluconacetobacter hansenii CGMCC1671)和啤酒酵母(Saccharomyces cerevisiae CGMCC1670),所用培养基为糖茶水培养基。研究了碳源、茶叶种类、茶叶用量、种龄、温度和装液量等6个因素对红茶菌合成BC的影响。静止培养22d后测BC的产量和产率。结果表明红茶菌合成BC的最佳碳源为葡萄糖,最佳茶叶种类为绿茶,最佳茶叶用量为4g/L,最佳种龄为60h,最佳温度为30℃,最佳装液量为250mL的三角瓶中装75mL的培养液。这些最佳参数和红茶菌的培养相一致。纯菌种培养红茶菌技术可以应用于生产高品质及高产量的BC和高品质的红茶菌饮料。%Strains Gluconacetobacter hansenii CGMCC1671 and Saccharomyces cerevisiae CGMCC1670 were applied to make traditional Kombucha with pure cultures to search for the optimum parameters of major factors affecting the yields and productivities of bacterial cellulose(BC) in the beverage.Six culture factors were examined:carbon sources,tea kind,tea dosage,inoculum age,temperature and culture volume.The yields and productivities of BC and sugar consumed were measured after cultured statically for 22d.Glucose at 50g/L,green tea at 4g/L,inoculum of 60h,30℃ and 75mL were the optimum conditions for high yield of BC.These optimum parameters were nearly equal for both the maturation of Kombucha and the BC yields.The co-culture of pure strains of traditional Kombucha technique can be used to provide both high quality and high yield of BC in addition to producing high quality Kombucha beverage.

  19. Antibacterial properties of nine pure metals: a laboratory study using Staphylococcus aureus and Escherichia coli.

    Science.gov (United States)

    Yasuyuki, Miyano; Kunihiro, Koyama; Kurissery, Sreekumari; Kanavillil, Nandakumar; Sato, Yoshiro; Kikuchi, Yasushi

    2010-10-01

    Bacterial attachment and growth on material surfaces are considered to be the primary steps leading to the formation of biofilm. Biofilms in hospital and food processing settings can result in bacterial infection and food contamination, respectively. Prevention of bacterial attachment, therefore, is considered to be the best strategy for abating these menaces and therefore the development of antibacterial metals becomes important. In this study, nine pure metals, viz. titanium, cobalt, nickel, copper, zinc, zirconium, molybdenum, tin, and lead have been tested for their antibacterial properties against two bacterial strains, Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli. This was accomplished using two assay methods, the film contact method and the shaking flask method. The results show that the antibacterial properties varied significantly with different metals and the effectiveness of metals to resist bacterial attachment varied with the bacterial strain. Among the metals tested, titanium and tin did not exhibit antibacterial properties. TEM images showed that metal accumulation resulted in the disruption of the bacterial cell wall and other cellular components.

  20. Pontine infarction with pure motor hemiparesis or hemiplegia: A prospective study

    Science.gov (United States)

    Ling, Li; Zhu, Liangfu; Zeng, Jinsheng; Liao, Songjie; Zhang, Suping; Yu, Jian; Yang, Zhiyun

    2009-01-01

    Background The study aimed to prospectively observe the clinical and neuroimaging features of pontine infarction with pure motor hemiparesis (PMH) or hemiplegia at early stage. Methods In 118 consecutive selected patients with the first-ever ischemic stroke within 6 hours after onset, fifty of them presented with PMH or hemiplegia and had negative acute computed tomography (CT) scans, then magnetic resonance imaging (MRI) confirmed the corresponding infarcts in pons or cerebrum. The clinical and neuroimaging features of the pontine infarctions were compared with those of cerebral infarctions. Results The pontine infarction with PMH or hemiplegia accounted for 10.2% (12/118) of all first-ever ischemic stroke patients and 24% (12/50) of the patients with both PMH or hemiplegia and acute negative CT scans. Compared to the patients with cerebral infarction, the patients with pontine infarction had more frequency of diabetes mellitus (50.0% vs 5.3%, P = 0.001), nonvertiginous dizziness at onset (58.3% vs 21.1%, P = 0.036) and a progressive course (33.3% vs 2.6%, P = 0.011). Conclusion The pontine infarction may present as PMH or hemiplegia with more frequency of nonvertiginous dizziness, a progressive course and diabetes mellitus. MRI can confirm the infarct location in the basal pons at early stage after stroke onset. PMID:19527495

  1. p53 in pure epithelioid PEComa: an immunohistochemistry study and gene mutation analysis.

    Science.gov (United States)

    Bing, Zhanyong; Yao, Yuan; Pasha, Theresa; Tomaszewski, John E; Zhang, Paul J

    2012-04-01

    Pure epithelioid PEComa (PEP; so-called epithelioid angiomyolipoma) is rare and is more often associated with aggressive behaviors. The pathogenesis of PEP has been poorly understood. The authors studied p53 expression and gene mutation in PEPs by immunohistochemistry, single-strand conformation polymorphism, and direct sequencing in paraffin material from 8 PEPs. A group of classic angiomyolipomas (AMLs) were also analyzed for comparison. Five PEPs were from kidneys and 1 each from the heart, the liver, and the uterus. PEPs showed much stronger p53 nuclear staining (Allred score 6.4 ± 2.5) than the classic AML (2.3 ± 2.9) (P CAC (coding amino acid changed from glutamine to histidine), and the other involving codon 182, with change from TGC to TAC (coding amino acid changed from cysteine to tyrosine). The finding of stronger p53 expression and mutations in epithelioid angiomyolipomas might have contributed to their less predictable behavior. However, the abnormal p53 expression cannot be entirely explained by p53 mutations in the exons examined in the PEPs.

  2. Comparison of pure laparoscopic versus open left hemihepatectomy by multivariate analysis: a retrospective cohort study.

    Science.gov (United States)

    Cho, Hwui-Dong; Kim, Ki-Hun; Hwang, Shin; Ahn, Chul-Soo; Moon, Deok-Bog; Ha, Tae-Yong; Song, Gi-Won; Jung, Dong-Hwan; Park, Gil-Chun; Lee, Sung-Gyu

    2017-07-21

    To compare the outcomes of pure laparoscopic left hemihepatectomy (LLH) versus open left hemihepatectomy (OLH) for benign and malignant conditions using multivariate analysis. All consecutive cases of LLH and OLH between October 2007 and December 2013 in a tertiary referral hospital were enrolled in this retrospective cohort study. All surgical procedures were performed by one surgeon. The LLH and OLH groups were compared in terms of patient demographics, preoperative data, clinical perioperative outcomes, and tumor characteristics in patients with malignancy. Multivariate analysis of the prognostic factors associated with severe complications was then performed. The LLH group (n = 62) had a significantly shorter postoperative hospital stay than the OLH group (n = 118) (9.53 ± 3.30 vs 14.88 ± 11.36 days, p Multivariate analysis revealed that the OLH group had >4 times the risk of the LLH group in terms of developing severe complications (Clavien-Dindo grade ≥III) (odds ratio 4.294, 95% confidence intervals 1.165-15.832, p = 0.029). LLH was a safe and feasible procedure for selected patients. LLH required shorter hospital stay and resulted in less operative blood loss. Multivariate analysis revealed that LLH was associated with a lower risk of severe complications compared to OLH. The authors suggest that LLH could be a reasonable treatment option for selected patients.

  3. Molecular dynamics study of strain-induced diffusivity of nitrogen in pure iron nanocrystalline

    Science.gov (United States)

    Mohammadzadeh, Roghayeh; Razmara, Naiyer; Razmara, Fereshteh

    2016-12-01

    In the present study, the self-diffusion process of nitrogen in pure iron nanocrystalline under strain conditions has been investigated by Molecular Dynamics (MD). The interactions between particles are modeled using Modified Embedded Atom Method (MEAM). Mean Square Displacement (MSD) of nitrogen in iron structure under strain is calculated. Strain is applied along [ 11 2 ¯ 0 ] and [ 0001 ] directions in both tensile and compression conditions. The activation energy and pre-exponential diffusion factor for nitrogen diffusion is comparatively high along [ 0001 ] direction of compressed structure of iron. The strain-induced diffusion coefficient at 973 K under the compression rate of 0.001 Å/ps along [ 0001 ] direction is about 6.72E-14 m2/s. The estimated activation energy of nitrogen under compression along [ 0001 ] direction is equal to 12.39 kcal/mol. The higher activation energy might be due to the fact that the system transforms into a more dense state when compressive stress is applied.

  4. Studying Legal Cultures and Encounters?

    DEFF Research Database (Denmark)

    Petersen, Hanne

    2015-01-01

    This article discusses the cultural and other turns in relation to legal culture and situates Western legal culture in context. It deals with concepts and their relations to trends and fashions and introduces methodological reflections such as use of interdisciplinary methods, personal experience...... and looks at the overlapping roles of authors as activists, teachers, communicators etc....

  5. We Need to Talk about Cultural Studies

    Directory of Open Access Journals (Sweden)

    Justin O'Connor

    2012-09-01

    Full Text Available A review of Graeme Turner, What’s Become of Cultural Studies (Sage, London, 2012 and Lawrence Grossberg, Cultural Studies in the Future Tense (Duke University Press, Durham, 2010.

  6. Development of Multiwell-Plate Methods Using Pure Cultures of Methanogens To Identify New Inhibitors for Suppressing Ruminant Methane Emissions.

    Science.gov (United States)

    Weimar, M R; Cheung, J; Dey, D; McSweeney, C; Morrison, M; Kobayashi, Y; Whitman, W B; Carbone, V; Schofield, L R; Ronimus, R S; Cook, G M

    2017-08-01

    Hydrogenotrophic methanogens typically require strictly anaerobic culturing conditions in glass tubes with overpressures of H2 and CO2 that are both time-consuming and costly. To increase the throughput for screening chemical compound libraries, 96-well microtiter plate methods for the growth of a marine (environmental) methanogen Methanococcus maripaludis strain S2 and the rumen methanogen Methanobrevibacter species AbM4 were developed. A number of key parameters (inoculum size, reducing agents for medium preparation, assay duration, inhibitor solvents, and culture volume) were optimized to achieve robust and reproducible growth in a high-throughput microtiter plate format. The method was validated using published methanogen inhibitors and statistically assessed for sensitivity and reproducibility. The Sigma-Aldrich LOPAC library containing 1,280 pharmacologically active compounds and an in-house natural product library (120 compounds) were screened against M. maripaludis as a proof of utility. This screen identified a number of bioactive compounds, and MIC values were confirmed for some of them against M. maripaludis and M. AbM4. The developed method provides a significant increase in throughput for screening compound libraries and can now be used to screen larger compound libraries to discover novel methanogen-specific inhibitors for the mitigation of ruminant methane emissions.IMPORTANCE Methane emissions from ruminants are a significant contributor to global greenhouse gas emissions, and new technologies are required to control emissions in the agriculture technology (agritech) sector. The discovery of small-molecule inhibitors of methanogens using high-throughput phenotypic (growth) screening against compound libraries (synthetic and natural products) is an attractive avenue. However, phenotypic inhibitor screening is currently hindered by our inability to grow methanogens in a high-throughput format. We have developed, optimized, and validated a high

  7. Study of a pure CsI crystal readout by APD for Belle II end cap ECL upgrade

    Energy Technology Data Exchange (ETDEWEB)

    Jin, Y., E-mail: jin@hep.phys.s.u-tokyo.ac.jp [Department of Physics, the University of Tokyo, 7-3-1 Hongo Bunkyo-ku, Tokyo 113-0033 (Japan); Aihara, H. [Department of Physics, the University of Tokyo, 7-3-1 Hongo Bunkyo-ku, Tokyo 113-0033 (Japan); Borshchev, O.V. [Enikolopov Institute of Synthetic Polymeric Materials of the Russian Academy of Sciences, Profsoyuznaya st. 70, Moscow 117393 (Russian Federation); Epifanov, D.A. [Department of Physics, the University of Tokyo, 7-3-1 Hongo Bunkyo-ku, Tokyo 113-0033 (Japan); Ponomarenko, S.A.; Surin, N.M. [Enikolopov Institute of Synthetic Polymeric Materials of the Russian Academy of Sciences, Profsoyuznaya st. 70, Moscow 117393 (Russian Federation)

    2016-07-11

    A scintillation counter consisting of a pure CsI crystal and avalanche photodiodes (Hamamatsu APD S8664-55 and S8664-1010) has been studied for the upgrade of the end cap electromagnetic calorimeter of Belle II detector. An essential increase of the light output was achieved with wavelength shifters based on nanostructured organosilicon luminophores. - Highlights: • A scintillation counter consisting of a pure CsI crystal and avalanche photodiodes has been studied. • The equivalent noise charge and equivalent noise energy of the counter have been measured. • An essential increase of the light output was achieved with wavelength shifters.

  8. Contributions to the phytocoenologic study in pure european beech stand forests in Codru-Moma Mountains (North-Western Romania

    Directory of Open Access Journals (Sweden)

    Călin-Gheorghe PĂŞCUŢ

    2010-05-01

    Full Text Available In the present work we present a phytocoenologic study on the associations found in pure European beech stand forests in Codru-Moma Mountains namely: Festuco drymejae-Fagetum Morariu et al. 1968, Luzulo albidae-Fagetum sylvaticae Zólyomi 1955.Characterization of the associations we studied and presentation of the tables have been made considering the selection of the most representative relevées of pure European beech forests belonging to Codru-Moma Mountains.The phytocoenoses of pure forest stands of European beech forests belonging to the two associations were analyzed in terms of floristic composition, life forms spectrum, spectrum chart of the floral elements and ecological indices.

  9. [A Clinicopathologic Study of 6 Patients with Histologically Pure Seminoma But Elevated Serum Alpha-Fetoprotein].

    Science.gov (United States)

    Kandori, Shuya; Kawai, Koji; Tanaka, Ken; Kawahara, Takashi; Ikeda, Atsushi; Ishitsuka, Ryutaro; Kimura, Tomokazu; Kojima, Takahiro; Joraku, Akira; Miyazaki, Jun; Nishiyama, Hiroyuki

    2017-04-01

    Histologically pure seminoma with elevated alpha-fetoprotein (AFP), so-called AFP-positive seminoma, is rare. It is recommended that patients with AFP-positive seminoma be managed as non-seminoma, but the clinical features and prognosis of this disease are not fully understood. In this study, we retrospectively analyzed 6 cases of metastatic AFP-positive seminoma at Tsukuba University Hospital (TUH). AFP was elevated before induction chemotherapy in 4 patients with an average of 1,372 ng/ml. In the remaining 2 patients, AFP became elevated during or after induction chemotherapy. In all 4 patients examined, AFPL3% was abnormally increased. As induction chemotherapy, all patients received bleomycin, etoposide and cisplatin (BEP), which was then followed by etoposide, ifosfamide and cisplatin (VIP) in 3 patients. After or during induction chemotherapy, 3 patients suffered from disease progression accompanied by AFP elevation. All 3 were treated by salvage chemotherapy and surgery. Four patients underwent retroperitoneal lymph node dissection (RPLND) after induction chemotherapy ; the pathological findings were necrosis in 3 patients, and viable nonseminomatous cancer in 1 patient. Furthermore, RPLND was performed as salvage surgery in 3 patients ; the pathological findings were necrosis, viable nonseminomatous cancer and teratoma with malignant transformation, respectively. The 5-year progression-free survival rate of the 6 patients was 50%, which is somewhat inferior to that of poor-prognosis non-seminoma patients treated at TUH. One patient ultimately died of cancer, and the remaining 5 are in remission with a median follow-up of 58 months. The present study demonstrates that AFP-positive seminoma patients have a higher risk of relapse compared to non-seminoma patients.

  10. Increased bacterial growth efficiency with environmental variability: results from DOC degradation by bacteria in pure culture experiments

    Directory of Open Access Journals (Sweden)

    M. Eichinger

    2010-06-01

    Full Text Available This paper assesses how considering variation in DOC availability and cell maintenance in bacterial models affects Bacterial Growth Efficiency (BGE estimations. For this purpose, we conducted two biodegradation experiments simultaneously. In experiment one, a given amount of substrate was added to the culture at the start of the experiment whilst in experiment two, the same amount of substrate was added, but using periodic pulses over the time course of the experiment. Three bacterial models, with different levels of complexity, (the Monod, Marr-Pirt and the dynamic energy budget – DEB – models, were used and calibrated using the above experiments. BGE has been estimated using the experimental values obtained from discrete samples and from model generated data. Cell maintenance was derived experimentally, from respiration rate measurements. The results showed that the Monod model did not reproduce the experimental data accurately, whereas the Marr-Pirt and DEB models demonstrated a good level of reproducibility, probably because cell maintenance was built into their formula. Whatever estimation method was used, the BGE value was always higher in experiment two (the periodically pulsed substrate as compared to the initially one-pulsed-substrate experiment. Moreover, BGE values estimated without considering cell maintenance (Monod model and empirical formula were always smaller than BGE values obtained from models taking cell maintenance into account. Since BGE is commonly estimated using constant experimental systems and ignore maintenance, we conclude that these typical methods underestimate BGE values. On a larger scale, and for biogeochemical cycles, this would lead to the conclusion that, for a given DOC supply rate and a given DOC consumption rate, these BGE estimation methods overestimate the role of bacterioplankton as CO2 producers.

  11. Increased bacterial growth efficiency with environmental variability: results from DOC degradation by bacteria in pure culture experiments

    Directory of Open Access Journals (Sweden)

    M. Eichinger

    2010-02-01

    Full Text Available This paper assesses how considering variation in DOC availability and cell maintenance in bacterial models affects Bacterial Growth Efficiency (BGE estimations. For this purpose, we conducted two biodegradation experiments simultaneously. In experiment one, a given amount of substrate was added to the culture at the start of the experiment whilst in experiment two, the same amount of substrate was added, but using periodic pulses over the time course of the experiment. Three bacterial models, with different levels of complexity, (the Monod, Marr-Pirt and the dynamic energy budget (DEB model, were used, and calibrated using the above experiments. BGE has been estimated using the experimental values obtained from discrete samples and from model generated data. Cell maintenance was derived experimentally, from respiration rate measurements. The results showed that the Monod model did not reproduce the experimental data accurately, whereas the Marr-Pirt and DEB models demonstrated a good level of reproducibility, probably because cell maintenance was built into their formula. Whatever estimation method was used, the BGE value was always higher in experiment two (the periodically pulsed substrate as compared to the initially one-pulsed-substrate experiment. Moreover, BGE values estimated without considering cell maintenance (Monod model and empirical formula were always smaller than BGE values obtained from models taking cell maintenance into account. Since BGE is commonly estimated using constant experimental systems and ignore maintenance, we conclude that these typical methods underestimate BGE values. On a larger scale, and for biogeochemical cycles, this would lead to the conclusion that, for a given DOC supply rate and a given DOC consumption rate, these BGE estimation methods overestimate the role of bacterioplankton as CO2 producers.

  12. Skin-pass rolling I – Studies on roughness transfer and elongation under pure normal loading

    DEFF Research Database (Denmark)

    Kijima, Hideo; Bay, Niels

    2008-01-01

    The influence of tool roughness in skin-pass or temper rolling of steel strip is investigated focusing on roughness transfer and strip elongation under pure normal loading, i.e. with no tangential shear between tool and workpiece. The process is simulated by an elasto-plastic FE simulation of plane...

  13. A STUDY OF HEARING IMPROVEMENT AFTER TYMPANOPLASTY BY MEANS OF PURE TONE AUDIOMETRY

    Directory of Open Access Journals (Sweden)

    Siddharth Nirwan

    2015-01-01

    Full Text Available BACKGROUND : Chronic S uppurative O titis M edia (CSOM is an important cause of preventable hearing loss , particularly in the developing world. Tympanoplasty is a procedure to eradicate the disease in middle ear and to reconstruct hearing mechanism. Pure tone audiometry is an efficient , simple and economic tool to assess the level of postoperative hearing gain

  14. Remote detection of laser-induced autofluorescence on pure cultures of fungal and bacterial strains and their analysis with multivariate techniques

    Science.gov (United States)

    Raimondi, Valentina; Palombi, Lorenzo; Cecchi, Giovanna; Lognoli, David; Trambusti, Massimo; Gomoiu, Ioana

    2007-05-01

    Remotely sensed laser-induced autofluorescence spectra of pure cultures of fungal strains ( Aureobasidium pullulans, Verticillium sp.) and of bacterial strains ( Bacillus sp., Pseudomonas sp.) are presented. The strains were isolated from samples collected in a Roman archaeological site ( Tropaeum Traiani) near Constanta, Romania. The fluorescence spectra were detected in vivo from a distance of 25 m in the outdoor, using a high spectral resolution fluorescence LIDAR featuring a UV laser (XeCl@308 nm) as an excitation source. All the examined strains, except for the A. pullulans, showed fluorescence features such to allow their characterisation by processing data with multivariate techniques. Both Principal Component Analysis and Cluster Analysis were applied to the data set and compared to discriminate between the examined strains. Results demonstrate the feasibility of fluorescence-based detection and characterisation of fungi and bacteria in the outdoor with a high spectral resolution fluorescence LIDAR. In addition, they show that the proposed processing methods offer a means to discriminate between the fluorescence features due to the investigated samples and that of a fluorescence background of a known spectral shape, as that of the culture medium. This can be exploited for the remote fluorescence mapping of heterotrophic organisms on stone surfaces when the latter show a typical broad fluorescence band.

  15. Ab initio LSDA and LSDA+U study of pure and Cd-doped cubic lanthanide sesquioxides

    DEFF Research Database (Denmark)

    Richard, D.; Muñoz, E.L.; Rentería, M.

    2013-01-01

    The electronic, structural, and hyperfine properties of pure and Cd-doped lanthanide (Ln) sesquioxides with the cubic bixbyite structure (Ln2O3, Ln ranging from La to Lu) have been studied using the full-potential augmented plane wave plus local orbital (APW+lo) method within the local spin density...

  16. Moving from High Culture to Ordinary Culture-On Raymond Wil-liams’Culture Study

    Institute of Scientific and Technical Information of China (English)

    陆育红

    2015-01-01

    Culture study has become a new trend in the academic fields, while how to define the scope and object of the cultural study, different people have different opinions. Ranging from Matthew Arnold’s Culture and Anarchy to Raymond Williams’The Long Revolution, scholars have been constantly exploring the core of cultural study of different periods. Raymond Williams had committed himself to the field of the study, and his study also has pushed ahead the people’s understanding of the theory. His con⁃tribution to the study has been analyzed here and that has brought a great influence to the study of the field.

  17. Nuclear magnetic resonance study of pure and Ni/Co doped LiFeAs

    Energy Technology Data Exchange (ETDEWEB)

    Grafe, Hans-Joachim; Baek, Seung-Ho; Hammerath, Franziska; Graefe, Uwe; Utz, Yannic; Harnagea, L.; Nacke, Claudia; Aswartham, Saicharan; Wurmehl, Sabine; Buechner, Bernd [Leibniz-Institut fuer Festkoerper- und Werkstoffforschung, Dresden (Germany)

    2011-07-01

    We present Nuclear Magnetic and Nuclear Quadrupole Resonance (NMR/NQR) measurements on pure, Ni and Co doped LiFeAs single crystals. The parent compound LiFeAs exhibits unconventional superconductivity with a transition temperature of about 17 K. Unlike other Fe based superconductors, where superconductivity is induced or stabilized by Co or Ni doping, replacement of Fe by these elements leads to a suppression of the superconducting transition temperature in LiFeAs. In case of Ni doping, a bulk magnetic order is induced below about 160 K. In contrast, for Co doping, the superconducting transition temperature is only reduced, but no magnetic order is observed. We discuss the nature and the origin of this magnetic order and its relation to unconventional superconductivity in pure LiFeAs.

  18. Study of Politeness in Cross Cultural Communication

    Institute of Scientific and Technical Information of China (English)

    桂仁娜; 姜艳

    2012-01-01

      Politeness is a universal linguistic phenomenon but also relative by nature and subject to culture. The politeness in pragmatic study and its cultural specificity in cross cultural communication are explained. Some tactics in cross communication are also suggested.

  19. Monitoring of glucose, salt and pure water in human whole blood: An in vitro study.

    Science.gov (United States)

    Imran, Muhammad; Ullah, Hafeez; Akhtar, Munir; Sial, Muhammad Aslam; Ahmed, Ejaz; Durr-E-Sabeeh; Ahmad, Mukhtar; Hussain, Fayyaz

    2016-07-01

    Designing and implementation of non-invasive methods for glucose monitoring in blood is main focus of biomedical scientists to provide a relief from skin puncturing of diabete patient. The objective of this research work is to investigate the shape deformations and the aggregation of red blood cells (RBCs) in the human blood after addition of three different analytes i) (0mM-400mM: Range) of glucose (C(6)H(12)O(6)), ii) (0mM-400mM: range) of pure salt (NaCl) and iii) (0mM- 350mM: range) of pure water (H(2)O). We have observed that the changes in the shape of individual cells from biconcave discs to spherical shapes and eventually the lysis of the cells at optimum concentration of glucose, salts and pure water. This demonstration also provides a base line to facilitate diabetes during partial diagnosis and monitoring of the glucose levels qualitatively both in research laboratories and clinical environment.

  20. Biodegradable polymer for sealing porous PEO layer on pure magnesium: An in vitro degradation study

    Science.gov (United States)

    Alabbasi, Alyaa; Mehjabeen, Afrin; Kannan, M. Bobby; Ye, Qingsong; Blawert, Carsten

    2014-05-01

    An attempt was made to seal the porous silicate-based plasma electrolytic oxidation (PEO) layer on pure magnesium (Mg) with a biodegradable polymer, poly(L-lactide) (PLLA), to delay the localized degradation of magnesium-based implants in body fluid for better in-service mechanical integrity. Firstly, a silicate-based PEO coating on pure magnesium was performed using a pulsed constant current method. In order to seal the pores in the PEO layer, PLLA was coated using a two-step spin coating method. The performance of the PEO-PLLA Mg was evaluated using electrochemical impedance spectroscopy (EIS) and potentiodynamic polarization. The EIS results showed that the polarization resistance (Rp) of the PEO-PLLA Mg was close to two orders of magnitude higher than that of the PEO Mg. While the corrosion current density (icorr) of the pure Mg was reduced by 65% with the PEO coating, the PEO-PLLA coating reduced the icorr by almost 100%. As expected, the Rp of the PEO-PLLA Mg decreased with increase in exposure time. However, it was noted that the Rp of the PEO-PLLA Mg even after 100 h was six times higher than that of the PEO Mg after 48 h exposure, and did not show any visible localized attack.

  1. Rehabilitation of pure alexia

    DEFF Research Database (Denmark)

    Starrfelt, Randi; Ólafsdóttir, Rannveig Rós; Arendt, Ida-Marie

    2013-01-01

    Acquired reading problems caused by brain injury (alexia) are common, either as a part of an aphasic syndrome, or as an isolated symptom. In pure alexia, reading is impaired while other language functions, including writing, are spared. Being in many ways a simple syndrome, one would think...... that pure alexia was an easy target for rehabilitation efforts. We review the literature on rehabilitation of pure alexia from 1990 to the present, and find that patients differ widely on several dimensions like alexia severity, and associated deficits. Many patients reported to have pure alexia...... in the reviewed studies, have associated deficits like agraphia or aphasia and thus do not strictly conform to the diagnosis. Few studies report clear and generalisable effects of training, none report control data, and in many cases the reported findings are not supported by statistics. We can, however...

  2. Rehabilitation of pure alexia

    DEFF Research Database (Denmark)

    Starrfelt, Randi; Ólafsdóttir, Rannveig Rós; Arendt, Ida-Marie

    2013-01-01

    that pure alexia was an easy target for rehabilitation efforts. We review the literature on rehabilitation of pure alexia from 1990 to the present, and find that patients differ widely on several dimensions like alexia severity, and associated deficits. Many patients reported to have pure alexia......Acquired reading problems caused by brain injury (alexia) are common, either as a part of an aphasic syndrome, or as an isolated symptom. In pure alexia, reading is impaired while other language functions, including writing, are spared. Being in many ways a simple syndrome, one would think...... in the reviewed studies, have associated deficits like agraphia or aphasia and thus do not strictly conform to the diagnosis. Few studies report clear and generalisable effects of training, none report control data, and in many cases the reported findings are not supported by statistics. We can, however...

  3. Antimicrobial activity of essential oils and five terpenoid compounds against Campylobacter jejuni in pure and mixed culture experiments.

    Science.gov (United States)

    Kurekci, Cemil; Padmanabha, Jagadish; Bishop-Hurley, Sharon L; Hassan, Errol; Al Jassim, Rafat A M; McSweeney, Christopher S

    2013-09-16

    The aim of this study was to examine the antimicrobial potential of three essential oils (EOs: tea tree oil, lemon myrtle oil and Leptospermum oil), five terpenoid compounds (α-bisabolol, α-terpinene, cineole, nerolidol and terpinen-4-ol) and polyphenol against two strains of Campylobacter jejuni (ACM 3393 and the poultry isolate C338), Campylobacter coli and other Gram negative and Gram positive bacteria. Different formulations of neem oil (Azadirachta indica) with these compounds were also tested for synergistic interaction against all organisms. Antimicrobial activity was determined by the use of disc diffusion and broth dilution assays. All EOs tested were found to have strong antimicrobial activity against Campylobacter spp. with inhibitory concentrations in the range 0.001-1% (v/v). Among the single compounds, terpinen-4-ol showed the highest activity against Campylobacter spp. and other reference strains. Based on the antimicrobial activity and potential commerciality of these agents, lemon myrtle oil, α-tops (α-terpineol+cineole+terpinen-4-ol) and terpinen-4-ol were also evaluated using an in vitro fermentation technique to test antimicrobial activity towards C. jejuni in the microbiota from the chicken-caecum. EO compounds (terpinen-4-ol and α-tops) were antimicrobial towards C. jejuni at high doses (0.05%) without altering the fermentation profile. EOs and terpenoid compounds can have strong anti-Campylobacter activity without adversely affecting the fermentation potential of the chicken-caeca microbiota. EOs and their active compounds may have the potential to control C. jejuni colonisation and abundance in poultry.

  4. preliminary study of American legal culture

    Institute of Scientific and Technical Information of China (English)

    周杨

    2016-01-01

    This thesis is a preliminary study of American legal culture. Nowadays more and more scholars see the importance of legal culture and do studies on it. The author selects a number of American legal TV plays and movies; analyzes their topics, main characters, and plots; then finds out why United States adopt the legal system; what kind of legal culture it has; and how the legal culture is reflected in those movies and TV plays. The author hopes this thesis can work not only as an insight into the American legal culture, but also a source of reference for china to improve its legal system.

  5. Out of Context: Thinking Cultural Studies Diasporically

    Directory of Open Access Journals (Sweden)

    Grant Farred

    2011-04-01

    Full Text Available This essay on cultural studies and the African Diaspora argues for a rethinking of cultural studies in two critical ways: firstly, that cultural studies, from its founding institutional and conceptual moment, cannot but be thought diasporically; and, secondly, that cultural studies be thought ‘out of’, or, against, context—that is, cultural studies is most revealing in its political and literary articulation when it is not read, as many of its advocates claim, contextually. This essay offers a broad critique of cultural studies and the (African diaspora but derives its most cogent and creative argument from its ability to read together the work of two diasporic authors, deracinated South African and Australian writers, J. M. Coetzee and David Malouf.

  6. Biodegradable polymer for sealing porous PEO layer on pure magnesium: An in vitro degradation study

    Energy Technology Data Exchange (ETDEWEB)

    Alabbasi, Alyaa; Mehjabeen, Afrin [Biomaterials and Engineering Materials (BEM) Laboratory, James Cook University, Townsville 4811, Queensland (Australia); Kannan, M. Bobby, E-mail: bobby.mathan@jcu.edu.au [Biomaterials and Engineering Materials (BEM) Laboratory, James Cook University, Townsville 4811, Queensland (Australia); Ye, Qingsong [Discipline of Dentistry, James Cook University, Townsville 4811, Queensland (Australia); Blawert, Carsten [Magnesium Innovation Centre, Institute of Materials Research, Helmholtz-Zentrum Geesthacht, Geesthacht 21502 (Germany)

    2014-05-01

    Graphical abstract: - Highlights: • Poly(L-lactide) was used to seal the porous PEO layer on Mg. • The dual-layer coating improved the in vitro degradation resistance of Mg. • Localized degradation was inhibited in the dual-layer coated Mg. - Abstract: An attempt was made to seal the porous silicate-based plasma electrolytic oxidation (PEO) layer on pure magnesium (Mg) with a biodegradable polymer, poly(L-lactide) (PLLA), to delay the localized degradation of magnesium-based implants in body fluid for better in-service mechanical integrity. Firstly, a silicate-based PEO coating on pure magnesium was performed using a pulsed constant current method. In order to seal the pores in the PEO layer, PLLA was coated using a two-step spin coating method. The performance of the PEO–PLLA Mg was evaluated using electrochemical impedance spectroscopy (EIS) and potentiodynamic polarization. The EIS results showed that the polarization resistance (R{sub p}) of the PEO–PLLA Mg was close to two orders of magnitude higher than that of the PEO Mg. While the corrosion current density (i{sub corr}) of the pure Mg was reduced by 65% with the PEO coating, the PEO–PLLA coating reduced the i{sub corr} by almost 100%. As expected, the R{sub p} of the PEO–PLLA Mg decreased with increase in exposure time. However, it was noted that the R{sub p} of the PEO–PLLA Mg even after 100 h was six times higher than that of the PEO Mg after 48 h exposure, and did not show any visible localized attack.

  7. Comparative studies on the growth of pinus brutia in pure and mixed plantations

    Energy Technology Data Exchange (ETDEWEB)

    Hasan, S.M.; Al-Saraf, M.J.; Khalil, M.T.

    1980-01-01

    In trials in a semi-arid poor soil in Hammam Al-Alil, Mosul, Iraq, 2-year-old P. brutia transplants were planted in two blocks at 1x1 spacing. One block was interplanted with 1-year-old Acacia farnesiana. There was n.s.d. in growth of P. brutia between the 2 blocks at the end of the first or second year, but height growth in the mixed stand was significantly greater than in the pure stand after 3 growing seasons. (Refs. 10).

  8. Study of localization in a void-sheet under stress states near pure shear

    DEFF Research Database (Denmark)

    Tvergaard, Viggo

    2015-01-01

    to the principal tensile stress. A number of previous investigations have shown that such imperfections can lead to plastic flow localization in a shear band and subsequently lead to void-sheet fracture inside the band. The present analyses confirm that the imperfection results in localization failure, even......-cracks. For the largest imperfection considered, i.e. a relatively large ratio of the void radius to void spacing, there is still a range of stress states in the vicinity of pure shear, where no localization is predicted, and for smaller imperfections this range is larger....

  9. Enumeration of total bacteria and bacteria with genes for proteolytic activity in pure cultures and in environmental samples by quantitative PCR mediated amplification.

    Science.gov (United States)

    Bach, H-J; Tomanova, J; Schloter, M; Munch, J C

    2002-05-01

    Real-time quantitative PCR assays were developed for the absolute quantification of different groups of bacteria in pure cultures and in environmental samples. 16S rRNA genes were used as markers for eubacteria, and genes for extracellular peptidases were used as markers for potentially proteolytic bacteria. For the designed 16S rDNA TaqMan assay, specificity of the designed primer-probe combination for eubacteria, a high amplification efficiency over a wide range of starting copy numbers and a high reproducibility is demonstrated. Cell concentrations of Bacillus cereus, B. subtilis and Pseudomonas fluorescens in liquid culture were monitored by TaqMan-PCR using the 16S rDNA target sequence of Escherichia coli as external standard for quantification. Results agree with plate counts and microscopic counts of DAPI stained cells. The significance of 16S rRNA operon multiplicity to the quantification of bacteria is discussed.Furthermore, three sets of primer pair together with probe previously designed for targeting different classes of bacterial extracellular peptidases were tested for their suitability for TaqMan-PCR based quantification of proteolytic bacteria. Since high degeneracy of the probes did not allow accurate quantification, SybrGreen was used instead of molecular probes to visualize and quantify PCR products during PCR. The correlation between fluorescence and starting copy number was of the same high quality as for the 16S rDNA TaqMan assay for all the three peptidase gene classes. The detected amount of genes for neutral metallopeptidase of B. cereus, for subtilisin of B. subtilis and for alkaline metallopeptidase of P. fluorescens corresponded exactly to the numbers of bacteria investigated by the 16S rDNA targeting assay. The developed assays were applied for the quantification of bacteria in soil samples.

  10. Comparative Pharmacokinetic Study of Mangiferin After Oral Administration of Pure Mangiferin and US Patented Polyherbal Formulation to Rats

    OpenAIRE

    Kammalla, Ananth Kumar; Ramasamy, Mohan Kumar; Inampudi, Jyothi; Dubey, Govind Prasad; Agrawal, Aruna; Kaliappan, Ilango

    2014-01-01

    The US patented polyherbal formulation for the prevention and management of type II diabetes and its vascular complications was used for the present study. The xanthone glycoside mangiferin is one of the major effector constituents in the Salacia species with potential anti-diabetic activity. The pharmacokinetic differences of mangiferin following oral administration of pure mangiferin and polyherbal formulation containing Salacia species were studied with approximately the same dose 30 mg/kg...

  11. Optimization of a high-throughput CTAB-based protocol for the extraction of qPCR-grade DNA from rumen fluid, plant and bacterial pure cultures.

    Science.gov (United States)

    Minas, Konstantinos; McEwan, Neil R; Newbold, Charles Jamie; Scott, Karen P

    2011-12-01

    The quality and yield of extracted DNA are critical for the majority of downstream applications in molecular biology. Moreover, molecular techniques such as quantitative real-time PCR (qPCR) are becoming increasingly widespread; thus, validation and cross-laboratory comparison of data require standardization of upstream experimental procedures. DNA extraction methods depend on the type and size of starting material(s) used. As such, the extraction of template DNA is arguably the most significant variable when cross-comparing data from different laboratories. Here, we describe a reliable, inexpensive and rapid method of DNA purification that is equally applicable to small or large scale or high-throughput purification of DNA. The protocol relies on a CTAB-based buffer for cell lysis and further purification of DNA with phenol : chloroform : isoamyl alcohol. The protocol has been used successfully for DNA purification from rumen fluid and plant cells. Moreover, after slight alterations, the same protocol was used for large-scale extraction of DNA from pure cultures of Gram-positive and Gram-negative bacteria. The yield of the DNA obtained with this method exceeded that from the same samples using commercial kits, and the quality was confirmed by successful qPCR applications.

  12. Thermal, dielectric and photoconductivity studies on pure, Mg{sup 2+} and Zn{sup 2+} doped BTCC single crystals

    Energy Technology Data Exchange (ETDEWEB)

    Selvakumar, S. [Department of Physics, Loyola College, Chennai 600034 (India); Rajasekar, S.A. [Department of Physics, Magna College of Engineering, Chennai 600055 (India); Thamizharasan, K. [Department of Physics, Sir Theagaraya College, Chennai 600021 (India); Sivanesan, S. [Department of Chemical Engineering, Anna University, Chennai 600025 (India); Ramanand, A. [Department of Physics, Loyola College, Chennai 600034 (India); Sagayaraj, P. [Department of Physics, Loyola College, Chennai 600034 (India)]. E-mail: psagayaraj@hotmail.com

    2005-10-15

    Single crystals of pure and doped bis(thiourea) cadmium chloride (BTCC) belonging to semiorganic nonlinear optical materials are grown by slow evaporation technique. The lattice parameters of the grown crystals are determined by single-crystal XRD. Single crystal XRD studies reveal that the incorporation of metallic dopants has not changed the structure of the parent crystal. The TGA traces of doped BTCC crystals indicate a marginal increase in the thermal stability of the samples. The response of dielectric constant in the frequency region of 500 Hz to 500 kHz has been investigated and the influence of metal substitution on the dielectric behaviour has been reported. Photoconductivity studies of pure and doped BTCC crystals revealed the positive photoconducting nature. Optical absorption studies indicate the low percentage of absorption of doped crystals in the visible region, thereby confirming the enhancement of NLO property.

  13. Benzoic Acid and Chlorobenzoic Acids: Thermodynamic Study of the Pure Compounds and Binary Mixtures With Water.

    Science.gov (United States)

    Reschke, Thomas; Zherikova, Kseniya V; Verevkin, Sergey P; Held, Christoph

    2016-03-01

    Benzoic acid is a model compound for drug substances in pharmaceutical research. Process design requires information about thermodynamic phase behavior of benzoic acid and its mixtures with water and organic solvents. This work addresses phase equilibria that determine stability and solubility. In this work, Perturbed-Chain Statistical Associating Fluid Theory (PC-SAFT) was used to model the phase behavior of aqueous and organic solutions containing benzoic acid and chlorobenzoic acids. Absolute vapor pressures of benzoic acid and 2-, 3-, and 4-chlorobenzoic acid from literature and from our own measurements were used to determine pure-component PC-SAFT parameters. Two binary interaction parameters between water and/or benzoic acid were used to model vapor-liquid and liquid-liquid equilibria of water and/or benzoic acid between 280 and 413 K. The PC-SAFT parameters and 1 binary interaction parameter were used to model aqueous solubility of the chlorobenzoic acids. Additionally, solubility of benzoic acid in organic solvents was predicted without using binary parameters. All results showed that pure-component parameters for benzoic acid and for the chlorobenzoic acids allowed for satisfying modeling phase equilibria. The modeling approach established in this work is a further step to screen solubility and to predict the whole phase region of mixtures containing pharmaceuticals.

  14. Molecular dynamics study of the mechanical loss in amorphous pure and doped silica

    Energy Technology Data Exchange (ETDEWEB)

    Hamdan, Rashid; Trinastic, Jonathan P.; Cheng, H. P., E-mail: cheng@qtp.ufl.edu [Department of Physics and Quantum Theory Project, University of Florida, Gainesville, Florida 32611 (United States)

    2014-08-07

    Gravitational wave detectors and other precision measurement devices are limited by the thermal noise in the oxide coatings on the mirrors of such devices. We have investigated the mechanical loss in amorphous oxides by calculating the internal friction using classical, atomistic molecular dynamics simulations. We have implemented the trajectory bisection method and the non-local ridge method in the DL-POLY molecular dynamics simulation software to carry out those calculations. These methods have been used to locate the local potential energy minima that a system visits during a molecular dynamics trajectory and the transition state between any two consecutive minima. Using the numerically calculated barrier height distributions, barrier asymmetry distributions, relaxation times, and deformation potentials, we have calculated the internal friction of pure amorphous silica and silica mixed with other oxides. The results for silica compare well with experiment. Finally, we use the numerical calculations to comment on the validity of previously used theoretical assumptions.

  15. Experimental and Simulation Studies of the Toluene on Pure-Silica MEL Zeolite

    DEFF Research Database (Denmark)

    Sanchez-Gil, Vicente; Noya, Eva G.; Sanz, Alejandro

    2016-01-01

    In this work, the adsorption of toluene on pure silica MEL zeolite is investigated using a variety of experimental and simulation methods. First, we measured the volumetric and calorimetric isotherms at 301 K and at 315 K. The volumetric adsorption isotherm presents a substep at a loading......-ray powder diffraction experiments of the zeolite at three different loads: empty, at half load (before the substep), and at high load (after the substep). Numerous new low intensity peaks and splittings of existing peaks at the empty and half-loaded diffractograms appear in the diffraction pattern...... of the high load sample. Atomic structural models compatible with the experimental spectra were obtained by performing N-reverse Monte Carlo simulations. Whereas at half load a good fit of the experimental diffraction pattern can be obtained using the rigid zeolite approximation, at high load, this is only...

  16. Cultural Studies of Science Education

    OpenAIRE

    El-Hani, Charbel Niño; Muñoz, Yupanqui J.

    2012-01-01

    Texto completo: acesso restrito. p. 909-943 Video games, as technological and cultural artifacts of considerable influence in the contemporary society, play an important role in the construction of identities, just as other artifacts (e.g., books, newspapers, television) played for a long time. In this paper, we discuss this role by considering video games under two concepts, othering and technopoly, and focus on how these concepts demand that we deepen our understanding of the ethics of v...

  17. Studies of ferroelectric and dielectric properties of pure and doped barium titanate prepared by sol-gel method

    Energy Technology Data Exchange (ETDEWEB)

    Bisen, Supriya; Mishra, Ashutosh; Jarabana, Kanaka M. [School of Physics, Vigyan Bhawan, Devi Ahilya University, Khandwa Road Indore-452001 (India)

    2016-05-23

    In this work, Barium Titanate (BaTiO{sub 3}) powders were synthesized via Sol-Gel auto combustion method using citric acid as a chelating agent. We study the behavior of ferroelectric and dielectric properties of pure and doped BaTiO{sub 3} on different concentration. To understand the phase and structure of the powder calcined at 900°C were characterized by X-ray Diffraction shows that tetragonal phase is dominant for pure and doped BTO and data fitted by Rietveld Refinement. Electric and Dielectric properties were characterized by P-E Hysteresis and Dielectric measurement. In P-E measurement ferroelectric loop tracer applied for different voltage. The temperature dependant dielectric constant behavior was observed as a function of frequency recorded on hp-Hewlett Packard 4192A, LF impedance, 5Hz-13Hz analyzer.

  18. In Vitro Skin Diffusion Study of Pure Forskolin versus a Forskolin-Containing Plectranthus barbatus Root Extract

    Science.gov (United States)

    Chen, Jing; Hammell, Dana C.; Spry, Malinda; D’Orazio, John A.; Stinchcomb, Audra L.

    2016-01-01

    An in vitro skin diffusion study of pure forskolin (1) versus a 1-containing Plectranthus barbatus root extract (P. barbatus extract) in hairless guinea pig skin and human skin in a flow-through diffusion cell system was conducted and is being reported for the first time. Both topical agents were formulated in a solution of 70% ethanol and 30% propylene glycol (v/v). The results showed that forskolin can be delivered through the stratum corneum and that the flux of this compound was enhanced when 1 was delivered as a constituent of the P. barbatus extract as compared to an equivalent amount in pure form. These results suggest that the P. barbatus extract used contains permeation enhancement activity from other compound(s) contained in the crude root extract. It is possible that P. barbatus root extract may be used as an economical source of 1 to perform topical chemical manipulation of pigmentation in high-risk populations. PMID:19281221

  19. A study of annealing stages in commercial pure Cu using mechanical measurements and positron annihilation lifetime technique

    Energy Technology Data Exchange (ETDEWEB)

    Bishay, I.K. [Solid State Physics Department, National Research Centre, Dokki, Cairo (Egypt); Aly, E. Hassan, E-mail: emad_h_ali@yahoo.com [Department of Physics, Faculty of Science, Ain Shams University, Abbassia, Cairo 11566 (Egypt); Saadallah, F.A. [Solid State Physics Department, National Research Centre, Dokki, Cairo (Egypt)

    2010-06-25

    Mechanical property measurements, positron annihilation lifetime (PAL) measurements and metallographic observations, have been performed to study the isochronal annealing of commercial pure Cu in the temperature range from 25 up to 850 deg. C. A positive correlation has been found between positron lifetime ({tau}) and both the tensile strain ({Delta}L/L{sub 0}) and Vicker's microhardness (H{sub v}). This correlation shows the presence of three annealing stages in commercial pure Cu which are attributed to recovery, recrystallization, and grain growth. These different stages were studied by both pure tensile and combined torsion-tension deformation for samples pre-annealed at the different annealing stages. Plastic instability behavior is observed in the case of combined torsion-tension deformation. It is observed that the onset and disappearance of this instability depend on some parameters such as mode of deformation, applied axial tensile stress and pre-annealing temperature. The activation energy is found to be 0.5 eV for the recovery stage which is attributed to the energy for dislocation annihilation by glide or cross-slip. The recrystallization stage is a multi-energy stage (1.35, 1.6, and 1.71 eV) which is attributed to lattice diffusion or boundary diffusion.

  20. Traditional Music in Igbo Culture: A Case Study of Idu Cultural ...

    African Journals Online (AJOL)

    Traditional Music in Igbo Culture: A Case Study of Idu Cultural Dance of ... It further investigates among other things the history and performance of Idu cultural dance. ... remained the climax of every cultural and social event in Akpo community.

  1. Study the Effect of Magnesium Addition on Microstructure , Electrical Conductivity and some Mechanical Properties of Pure Aluminum

    Directory of Open Access Journals (Sweden)

    Nagham Yass Khudair

    2017-03-01

    Full Text Available In this research study the effect of addition of magnesium on the mechanical properties (Hardness , yield strength , Ultimate stress for pure aluminum and also microstructure , density , in addition to the electrical conductivity . three alloys were prepared by change ratios add magnesium ( 5% , 10%,15% . The results showed that the increase proportion of the magnesium added to pure aluminum caused an increase in the yield stress and improving ratio reach to 78.46% ,an increase in ultimate stress and improving ratio reach to76.92% at ratio(15% , and an increase in the hardness to value (91.9 Hv and improving ratio reach to 90.78% at ratio (15 % while the decrease in electrical conductivity to the value of (0.596 *106 -1 .m-1 while there is a decrease in the density to (2.371 g/cm3 when the ratio of magnesium added to the pure aluminum increases. A change in microstructure and regular distribution of magnesium on the boundary of crystalline for alloys , has also been noticed.

  2. Study on culturing Trichodema mutants

    Institute of Scientific and Technical Information of China (English)

    CHEN Jian-ai; WANG Wei-ming

    2004-01-01

    @@ Trichodema mutants strains T5, T0803, T1010, T1003were cultured in different conditions and media, also in the presence of fungicides at 40 mg/kg (CK or procymidone + chlorothalonil, or maneb or phosethyl-Al) . The pH values of media were 5, 6, 7 and 8 and hyphae were grown at temperatures of 15, 20, 25 and 30 ℃. After being cultured for 3, 4, 5, or 6 days, the strains were transferred at a lower temperature to sporulate (20℃) Obtained data were analyzed statistically, with the orthogonal array and ranges (R) differing dependes on the treatments (R = 40.0,42.4, 48.0, 62.8,107.0). The results indicated that the most important factor was the nature of the strain (R =107.0), while the change in temperature and time of cultivation produced the lowest effect (R =40.0). Each factor variance was significant and A3B4C2D1E3 was the optimum combined condition, in which strain T1010 grew more quickly and sporulated most.

  3. Re-imagining South African Cultural Studies

    African Journals Online (AJOL)

    Studies as, amongst other things, the study of areas of culture largely ignored ... the canon, its boundaries and functions ~ particularly in a context where the cul- .... the complicated relationship between subjects and their symbolic structures.

  4. Studies of Water V. Five Phonons in Protonic Semiconductor Lattice Model of Pure Liquid Water

    Science.gov (United States)

    Jie, Binbin; Sah, Chihtang

    2017-07-01

    We report physics based confirmation (~1% RMS deviation), by existing experimental data, of proton-prohol (proton-hole) ion product (pH) and mobilities in pure liquid water (0-100{}{{o}}C, 1-atm pressure) anticipated from our melted-ice Hexagonal-Close-Packed (H{}2O){}4 Lattice Model. Five phonons are identified. (1) A propagating protonic phonon (520.9 meV from lone-pair-blue-shifted stretching mode of isolated water molecule) absorbed to generate a proton-prohol pair or detrap a tightly-bound proton. (2) Two (173.4 and 196.6 meV) bending-breathing protonic-proholic or protonic phonons absorbed during de-trapping-limited proton or proton-prohol mobilities. (3) Two propagating oxygenic-wateric Debye-Dispersive phonons (30.3 and 27.5 meV) absorbed during scattering-limited proton or proton-prohol mobilities. Summer School in Theoretical Physics funded by the National Natural Science Foundation of China, on Soft Materials Physics, hosted by the Physics Department of Xiamen University, China, during August 1 to 14, 2016. This was also just presented at the 2017 March Meeting (March 14 to 16) of the American Physical Society in New Orleans, USA.

  5. Study of Cultural Negative Transfer in Cross-cultural Communication

    Institute of Scientific and Technical Information of China (English)

    国玲

    2015-01-01

    Different nations in the world have their own cultures, and these cultures are characterized by both universality and particularity. The former provides a foundation and guarantee for intercultural communication, while the latter often leads to neg⁃ative cultural transfer in communication if the speakers are unconscious of cultural differences. This paper makes a general analysis of the negative transfer of surface-structure culture in language forms and that of deep-structure culture in values, thought pat⁃terns, religious beliefs and ethics. It holds that failure in intercultural communication will occur if inadequate attention is paid to cultural differences in the process of language and culture learning.

  6. Review of Studies on Just Culture

    Energy Technology Data Exchange (ETDEWEB)

    Yi, Kyung Joo; Choi, Young Sung; Jung, Su Jin [Safety Policy Department, Korea Institute of Nuclear Safety, Daejeon (Korea, Republic of)

    2014-10-15

    Safety culture represents patterns of behavior and ways of thinking on safety for both individuals and organizations. Besides nuclear industry, many fields such as transport and health care have focused on the value and effectiveness of safety culture. In this paper, the definition and characteristics of just culture, which is one component of safety culture, and related studies reported by other researchers are described. We hope this review will serve to understand the current condition and underlying problems of the just culture of nuclear industry in Korea. This paper describes the definition and basic principle of just culture in nuclear industry based on the review of researches on safety and just culture in various fields. To establish just culture solidly in organization, each individual needs to understand the nature of the just culture first. And it is necessary to create an atmosphere encouraging to report and suggest safety-related issues. Most of all, to draw clear lines of blame and punishment for human error is essential.

  7. Fermentation of Dietetic Fiber from Green Bean and Prickly Pear Shell by Pure and Mixture Culture of Lactobacillus acidophilus LA-5 and Bifidobacterium bifidum 450B.

    Science.gov (United States)

    Mora-Cura, Y N; Meléndez-Rentería, N P; Delgado-García, M; Contreras-Esquivel, J C; Morlett-Chávez, J A; Aguilar, C N; Rodríguez-Herrera, R

    2017-03-23

    The aim of this study was to evaluate the fermentation of dietary fiber from green bean (Phaseolus vulgaris) and prickly pear shell (Opuntia ficus-indica) by Lactobacillus acidophilus LA-5 and Bifidobacterium bifidum 450B growing as mono-culture and co-culture, the fermentation products, and proteins expressed during this process. The analysis of the fermentation profile showed a major growth of bacteria in the culture media of each dietary fiber supplemented with glucose, and particularly B. bifidum 450B at 48 h showed the highest growth. In the case of the co-culture, the growth was lower indicating the possible negative interaction between L. acidophilus LA-5 and B. bifidum 450B and may be due to the less amount of carbohydrates and the high content of non-soluble fiber that affected the nutrients availability for the bacterial strains. The pH changes indicated the presence of short-chain fatty acids (SCFAs), being acetate (46-100%) the main SCFA. Changes in the proteome concerned proteins that are involved in carbohydrate and other carbohydrate pathways. The characterization of the bacteria according to the growth, metabolites, and proteins expressed allows understanding the response to the change of environmental conditions and could be useful to understand L. acidophilus LA-5 and B. bifidum 450B strains' adaptation to specific applications.

  8. Pure-tone Audiometer

    Science.gov (United States)

    Kapul, A. A.; Zubova, E. I.; Torgaev, S. N.; Drobchik, V. V.

    2017-08-01

    The research focuses on a pure-tone audiometer designing. The relevance of the study is proved by high incidence of an auditory analyser in older people and children. At first, the article provides information about subjective and objective audiometry methods. Secondly, we offer block-diagram and basic-circuit arrangement of device. We decided to base on STM32F407VG microcontroller and use digital pot in the function of attenuator. Third, we implemented microcontroller and PC connection. C programming language is used for microcontroller’s program and PC’s interface. Fourthly, we created the pure-tone audiometer prototype. In the future, we will implement the objective method ASSR in addition to pure-tone audiometry.

  9. Studies of discrete symmetries in a purely leptonic system using the Jagiellonian Positron Emission Tomograph

    Directory of Open Access Journals (Sweden)

    Moskal P.

    2016-01-01

    Full Text Available Discrete symmetries such as parity (P, charge-conjugation (C and time reversal (T are of fundamental importance in physics and cosmology. Breaking of charge conjugation symmetry (C and its combination with parity (CP constitute necessary conditions for the existence of the asymmetry between matter and antimatter in the observed Universe. The presently known sources of discrete symmetries violations can account for only a tiny fraction of the excess of matter over antimatter. So far CP and T symmetries violations were observed only for systems involving quarks and they were never reported for the purely leptonic objects. In this article we describe briefly an experimental proposal for the test of discrete symmetries in the decays of positronium atom which is made exclusively of leptons. The experiments are conducted by means of the Jagiellonian Positron Emission Tomograph (J-PET which is constructed from strips of plastic scintillators enabling registration of photons from the positronium annihilation. J-PET tomograph together with the positronium target system enable to measure expectation values for the discrete symmetries odd operators constructed from (i spin vector of the ortho-positronium atom, (ii momentum vectors of photons originating from the decay of positronium, and (iii linear polarization direction of annihilation photons. Linearly polarized positronium will be produced in the highly porous aerogel or polymer targets, exploiting longitudinally polarized positrons emitted by the sodium 22Na isotope. Information about the polarization vector of orthopositronium will be available on the event by event basis and will be reconstructed from the known position of the positron source and the reconstructed position of the orthopositronium annihilation. In 2016 the first tests and calibration runs are planned, and the data collection with high statistics will commence in the year 2017.

  10. Anartria Pura: Estudo de Dois Casos Pure Anartria: Study of Two Cases

    Directory of Open Access Journals (Sweden)

    André Roch Lecours

    2001-01-01

    Full Text Available O marco do nascimento da neurolingüística é considerado a publicação do livro A síndrome de Desintegração Fonética, em 1939, que teve como autores: um neurologista, um psicólogo e uma lingüista. Dez anos mais tarde foi publicado, pela mesma equipe de pesquisadores franceses, o distúrbio de fala do paciente portador de anartria pura. No presente artigo, situaremos as polêmicas históricas a respeito de um distúrbio seletivo de fala de origem telencefálica, apresentaremos o primeiro caso descrito, acompanhado da descrição anátomo-patológica do cérebro que estivera guardado em formol por 20 anos e, realizada por um de nós (ARL, e por fim, apresentaremos um caso brasileiro, cujas imagens tomográficas de seu cérebro e espectográficas de sua fala e voz confirmam o trabalho pioneiro de Alajouanine, Pichot e Durant.The mark of birth of neuro-linguistics is considered the publication of the book The Syndrome of Phonetic Disintegration, in 1939, that had as authors: a neurologist, a psychologist and a linguistics. Ten years later the same team of French researchers published about, the disturbance of speech of the patient carrier of pure Anartria. In the present article, we will place the historical polemics regarding a selective disturbance of speech of telencephalic origin and present the first described case, accompanied by the anatomic-pathological description of the brain that had been kept in formol by 20 years accomplished by one of us (ARL. Finally, we will present a Brazilian case, with tomographic images of its brain and spectrographic evaluation of its speech and voice that confirm the pioneer work of Alajouanine, Pichot and Durant.

  11. Studies of discrete symmetries in a purely leptonic system using the Jagiellonian Positron Emission Tomograph

    Science.gov (United States)

    Moskal, P.; Alfs, D.; Bednarski, T.; Białas, P.; Curceanu, C.; Czerwiński, E.; Dulski, K.; Gajos, A.; Głowacz, B.; Gupta-Sharma, N.; Gorgol, M.; Hiesmayr, B. C.; Jasińska, B.; Kamińska, D.; Khreptak, O.; Korcyl, G.; Kowalski, P.; Krzemień, W.; Krawczyk, N.; Kubicz, E.; Mohammed, M.; Niedźwiecki, Sz.; Pawlik-Niedńwiecka, M.; Raczyński, L.; Rudy, Z.; Silarski, M.; Smyrski, J.; Wieczorek, A.; Wiślicki, W.; Zgardzińska, B.; Zieliński, M.

    2016-11-01

    Discrete symmetries such as parity (P), charge-conjugation (C) and time reversal (T) are of fundamental importance in physics and cosmology. Breaking of charge conjugation symmetry (C) and its combination with parity (CP) constitute necessary conditions for the existence of the asymmetry between matter and antimatter in the observed Universe. The presently known sources of discrete symmetries violations can account for only a tiny fraction of the excess of matter over antimatter. So far CP and T symmetries violations were observed only for systems involving quarks and they were never reported for the purely leptonic objects. In this article we describe briefly an experimental proposal for the test of discrete symmetries in the decays of positronium atom which is made exclusively of leptons. The experiments are conducted by means of the Jagiellonian Positron Emission Tomograph (J-PET) which is constructed from strips of plastic scintillators enabling registration of photons from the positronium annihilation. J-PET tomograph together with the positronium target system enable to measure expectation values for the discrete symmetries odd operators constructed from (i) spin vector of the ortho-positronium atom, (ii) momentum vectors of photons originating from the decay of positronium, and (iii) linear polarization direction of annihilation photons. Linearly polarized positronium will be produced in the highly porous aerogel or polymer targets, exploiting longitudinally polarized positrons emitted by the sodium 22Na isotope. Information about the polarization vector of orthopositronium will be available on the event by event basis and will be reconstructed from the known position of the positron source and the reconstructed position of the orthopositronium annihilation. In 2016 the first tests and calibration runs are planned, and the data collection with high statistics will commence in the year 2017.

  12. The 3-second rule in hereditary pure cerebellar ataxia: a synchronized tapping study.

    Directory of Open Access Journals (Sweden)

    Shunichi Matsuda

    Full Text Available The '3-second rule' has been proposed based on miscellaneous observations that a time period of around 3 seconds constitutes the fundamental unit of time related to the neuro-cognitive machinery in normal humans. The aim of paper was to investigate the temporal processing in patients with spinocerebellar ataxia type 6 (SCA6 and SCA31, pure cerebellar types of spinocerebellar degeneration, using a synchronized tapping task. Seventeen SCA patients (11 SCA6, 6 SCA31 and 17 normal age-matched volunteers participated. The task required subjects to tap a keyboard in synchrony with sequences of auditory stimuli presented at fixed interstimulus intervals (ISIs between 200 and 4800 ms. In this task, the subjects required non-motor components to estimate the time of forthcoming tone in addition to motor components to tap. Normal subjects synchronized their taps to the presented tones at shorter ISIs, whereas as the ISI became longer, the normal subjects displayed greater latency between the tone and the tapping (transition zone. After the transition zone, normal subjects pressed the button delayed relative to the tone. On the other hand, SCA patients could not synchronize their tapping with the tone even at shorter ISIs, although they pressed the button delayed relative to the tone earlier than normal subjects did. The earliest time of delayed tapping appearance after the transition zone was 4800 ms in normal subjects but 1800 ms in SCA patients. The span of temporal integration in SCA patients is shortened compared to that in normal subjects. This could represent non-motor cerebellar dysfunction in SCA patients.

  13. An Exchange on Theory and Cultural Studies

    Directory of Open Access Journals (Sweden)

    Various Authors

    2013-08-01

    Full Text Available The following exchange grew out of a series of posts to the Cultural Studies Association of Australasia discussion list.1 As a talking point leading into a regular meeting for early career cultural studies researchers in Brisbane, Melissa Gregg, Jean Burgess and Joshua Green quoted a passage from Simon During’s recent Cultural Studies: A Critical Introduction (Routledge, 2005 in the hope of provoking a wider debate about the current state of Australian cultural studies. Various members of the list were duly provoked, and the ensuing discussion was later picked up in a paper by John Frow and continued in private correspondence and then in invited responses to the developing exchange.

  14. Children and media: a cultural studies approach

    OpenAIRE

    D av i d B u c k i n g h a m

    2012-01-01

    Defying the traditional psychology understanding on what are the effects of the media and also the researches made on the subject, the present article offers an approach to the study of the relationship between children and the media, focusing mainly on television. We retrace the Cultural Studies perspective, although the researches from the Birmingham Centre have not worked over such age group.The work includes the model of the cultural circuit, it refuses to understand meaning as something ...

  15. 石油降解单菌株及混合菌降解产物分析%Analysis biodegradation products of oil compounds by pure and mixed bacterial cultures

    Institute of Scientific and Technical Information of China (English)

    花莉; 彭香玉; 范洋; 解井坤

    2014-01-01

    Four oil-degrading bacteria strains ,isolated from oil-contaminated soil of Chan-gqing Oilfied Company ,were mixed to construct bacterial consortiums for crude oil degrada-tion .Isolated microorganisms ,in the form of pure and mixed ,were cultured for 5 days at 30 ℃ in a medium containing diesel .These pure bacteria degrade 41 .83% ~54 .87% of diesel and among them Klebsiella variicola showed the greatest capability for degradation diesel . Biodegradation of diesel by the mixed culture of four bacteria was 64 .27% .The degradability of bacterial mixed culture is higher than the four pure cultures .Raoultella planticola and K lebsiella v ariicola can degrade branched-alkene ,Serratia m arcescens and Bacillus cereus can oxidative unsaturated aldehyde to acid .Bacillus cereus and K lebsiella v ariicola trans-formed cycloalkanes into linear ester via a primary alcohol .In addition K lebsiella v ariicola degraded a triple bond between two carbon atoms (C≡C) of 7-Pentadecyne .It was a promi-nent strain in the oil degradation process .Microbial antagonism in four strains made them not suitable for degrading cycloalkanes .The research provides theoretical foundation for the study on metabolism of hydrocarbons and screen for functional microorganism .%从中国石油长庆石化公司附近的油泥中分离筛选出4株石油降解菌,用于组建降解原油的混合菌体系.在等接种量培养条件下,单菌株对石油烃的降解率达到41.83%~54.87%,而混合菌降解效率高于单菌株,达到64.27%.不同微生物在降解过程中起着不同的作用.居植物柔武士菌(Raoultella p lanticola)具有脱烷基功能,对一些支链烃有降解效果;蜡状芽孢杆菌(Bacillus cereus)既可以氧化末端烯烃,又可以降解环烷烃;克雷伯氏菌(K lebsiella v ari-icola)是优势菌种,不仅具有以上3种降解功能,还可以降解炔烃;粘质沙雷氏菌(Serratia marcescens

  16. Study of the Kroll-process to produce ultra-pure Ti for the low background experiments

    Energy Technology Data Exchange (ETDEWEB)

    Mozhevitina, Elena [D. Mendeleyev University of Chemical Technology of Russia, Moscow 125047 (Russian Federation); Chepurnov, Alexander [Lomonosov Moscow State University, Skobeltsyn Institute of Nuclear Physics, Moscow 119234 (Russian Federation); Chub, Alexander [Solikamsk Magnesium Plant, Solikamsk,618541 (Russian Federation); Avetissov, Igor [ARMOLED Ltd, Moscow 125047 (Russian Federation); Glebovsky, Vadim [Institute of Solid State Physics, Russian Academy of Sciences, Chernogolovka, 142432 (Russian Federation); Nisi, Stefano; Di Vacri, Maria Laura [INFN Laboratori Nazionali del Gran Sasso, SS 17 bis km 18+910, 6010 Assergi (Italy); Suvorov, Yury [Physics and Astronomy Department, University of California, Los Angeles, Los Angeles, CA 90095, U.S.A (United States); National Research Center Kurchatov Institute, Moscow 123182 (Russian Federation)

    2015-08-17

    To construct G2 & G3 low background experiments for direct Dark Matter search, a lot of ultra-pure construction materials will be needed. Our study of various Ti samples shows that the levels of contaminations of commercially available industrial Ti can varies from 0.2 to 100 mBq/kg for U/Th. It means that the metal Ti is such a promising material for future experiments if the way of production in bulk quantities with very low level of contaminations (below ∼1 mBq/kg of {sup 238}U /{sup 232}Th) would be developed. Our study of the industrial Kroll-process is focused on the possible sources of U and Th and their migration during the multistage Kroll process. To understand migration of U and Th during the Kroll process the set of other impurities available due to precise ICP-MS analysis have been studied. Preliminary results confirm that the Kroll process could be used for the ultra pure Ti sponge production while the following stages of the metal Ti production of from the Ti sponge with necessary mechanical properties need to be additionally studied.

  17. Macro-evolutionary studies of cultural diversity: a review of empirical studies of cultural transmission and cultural adaptation.

    Science.gov (United States)

    Mace, Ruth; Jordan, Fiona M

    2011-02-12

    A growing body of theoretical and empirical research has examined cultural transmission and adaptive cultural behaviour at the individual, within-group level. However, relatively few studies have tried to examine proximate transmission or test ultimate adaptive hypotheses about behavioural or cultural diversity at a between-societies macro-level. In both the history of anthropology and in present-day work, a common approach to examining adaptive behaviour at the macro-level has been through correlating various cultural traits with features of ecology. We discuss some difficulties with simple ecological associations, and then review cultural phylogenetic studies that have attempted to go beyond correlations to understand the underlying cultural evolutionary processes. We conclude with an example of a phylogenetically controlled approach to understanding proximate transmission pathways in Austronesian cultural diversity.

  18. Macro-evolutionary studies of cultural diversity: a review of empirical studies of cultural transmission and cultural adaptation

    Science.gov (United States)

    Mace, Ruth; Jordan, Fiona M.

    2011-01-01

    A growing body of theoretical and empirical research has examined cultural transmission and adaptive cultural behaviour at the individual, within-group level. However, relatively few studies have tried to examine proximate transmission or test ultimate adaptive hypotheses about behavioural or cultural diversity at a between-societies macro-level. In both the history of anthropology and in present-day work, a common approach to examining adaptive behaviour at the macro-level has been through correlating various cultural traits with features of ecology. We discuss some difficulties with simple ecological associations, and then review cultural phylogenetic studies that have attempted to go beyond correlations to understand the underlying cultural evolutionary processes. We conclude with an example of a phylogenetically controlled approach to understanding proximate transmission pathways in Austronesian cultural diversity. PMID:21199844

  19. Toxic influence of silver and uranium salts on activated sludge of wastewater treatment plants and synthetic activated sludge associates modeled on its pure cultures.

    Science.gov (United States)

    Tyupa, Dmitry V; Kalenov, Sergei V; Skladnev, Dmitry A; Khokhlachev, Nikolay S; Baurina, Marina M; Kuznetsov, Alexander Ye

    2015-01-01

    Toxic impact of silver and uranium salts on activated sludge of wastewater treatment facilities has been studied. Some dominating cultures (an active nitrogen fixer Agrobacterium tumifaciens (A.t) and micromyces such as Fusarium nivale, Fusarium oxysporum, and Penicillium glabrum) have been isolated and identified as a result of selection of the activated sludge microorganisms being steadiest under stressful conditions. For these cultures, the lethal doses of silver amounted 1, 600, 50, and 300 µg/l and the lethal doses of uranium were 120, 1,500, 1,000, and 1,000 mg/l, respectively. A.tumifaciens is shown to be more sensitive to heavy metals than micromyces. Synthetic granular activated sludge was formed on the basis of three cultures of the isolated micromyces steadiest against stress. Its granules were much more resistant to silver than the whole native activated sludge was. The concentration of silver causing 50 % inhibition of synthetic granular activated sludge growth reached 160-170 μg/l as far as for the native activated sludge it came only to 100-110 μg/l.

  20. Cultural Transduction and Adaptation Studies: The Concept of Cultural Proximity

    National Research Council Canada - National Science Library

    Patrick Cattrysse

    2017-01-01

    ...). [...]it expands the analytical scope. The author bases his claims on the observation that, in spite of cultural imperialist trends, audiences may prefer audiovisual programs from or about their own or a nearby culture...

  1. Repeated dose studies with pure Epigallocatechin-3-gallate demonstrated dose and route dependant hepatotoxicity with associated dyslipidemia

    Directory of Open Access Journals (Sweden)

    Balaji Ramachandran

    2016-01-01

    Full Text Available EGCG (Epigallocatechin-3-gallate is the major active principle catechin found in green tea. Skepticism regarding the safety of consuming EGCG is gaining attention, despite the fact that it is widely being touted for its potential health benefits, including anti-cancer properties. The lack of scientific data on safe dose levels of pure EGCG is of concern, while EGCG has been commonly studied as a component of GTE (Green tea extract and not as a single active constituent. This study has been carried out to estimate the maximum tolerated non-toxic dose of pure EGCG and to identify the treatment related risk factors. In a fourteen day consecutive treatment, two different administration modalities were compared, offering an improved [i.p (intraperitoneal] and limited [p.o (oral] bioavailability. A trend of dose and route dependant hepatotoxicity was observed particularly with i.p treatment and EGCG increased serum lipid profile in parallel to hepatotoxicity. Fourteen day tolerable dose of EGCG was established as 21.1 mg/kg for i.p and 67.8 mg/kg for p.o. We also observed that, EGCG induced effects by both treatment routes are reversible, subsequent to an observation period for further fourteen days after cessation of treatment. It was demonstrated that the severity of EGCG induced toxicity appears to be a function of dose, route of administration and period of treatment.

  2. Growth and studies of pure and potassium iodide-doped zinc tris-thiourea sulphate (ZTS) single crystals

    Science.gov (United States)

    Krishnan, C.; Selvarajan, P.; Freeda, T. H.

    2008-12-01

    Single crystals of pure and potassium iodide (KI)-doped Zinc Tris-thiourea Sulphate (ZTS) were grown from aqueous solutions by slow evaporation technique. The grown crystals have been subjected to single crystal X-ray diffraction to determine the unit cell dimensions. The grown crystals were also characterized by recording the powder X-ray diffraction patterns and by identifying the diffracting planes. The Fourier Transform Infrared (FT-IR) spectra have been recorded in the range 400-4500 cm -1. Second harmonic generation (SHG) for the materials of this work was confirmed using Nd:YAG laser. The UV-visible spectra show that the grown crystals have wide optical transparency in the entire visible region. The Thermogravimetric/Differential Thermal Analyses (TG/DTA) thermograms reveal that the materials have good thermal stability. Atomic absorption study reveals the presence of potassium in the doped f crystals. The electrical measurements were made in the temperature range 40-130 °C along c-direction of the grown crystals. The dielectric studies show that there may be a ferroelectric transition at 50 °C for both pure and KI-doped ZTS crystals. DC conductivity for both the samples is found to be increasing with increase in temperature. Activation energy values were also determined for both AC and DC conduction processes in the samples.

  3. Electrochemical impedance study on the corrosion of Al-Pure in hydrochloric acid solution using Schiff bases

    Indian Academy of Sciences (India)

    A S Patel; V A Panchal; N K Shah

    2012-04-01

    The inhibition effect of newly synthesized Schiff bases -benzylidene benzylamine (A) and benzenemethanamine--methyl--(phenylmethylene) (B) on the corrosion behaviour of Al-Pure in 1.0 M HCl was studied using galvanostatic polarization and electrochemical impedance spectroscopy (EIS) and adsorption studies. The effects of inhibitor concentration, temperature and surface coverage are investigated. The effect of inhibitor concentration and other parameters are evaluated for different inhibitor concentrations and the probable mechanism was also proposed. The results show that (A) and (B) possess excellent inhibiting effect for the corrosion of Al-Pure and the inhibitors act as mixed type inhibitors. The inhibitors do not affect the mechanism of the electrode processes and inhibit corrosion by blocking the reaction sites. The high inhibition efficiency of (A) and (B) were due to the adsorption of inhibitor molecules on the metal surface. The decrease of surface area available for electrode reactions to take place is due to the formation of a protective film. Activation energy and free energy of adsorption have been calculated.

  4. Cultural Studies and Media Ecology: Meyrowitz's Medium Theory and Carey's Cultural Studies.

    Science.gov (United States)

    Flayhan, Donna P.

    2001-01-01

    Examines work of two communication and media studies scholars, Joshua Meyrowitz and James Carey. Suggests their studies represent media ecology with analyses of the dynamic interaction between communication, consciousness, and culture. Highlights how their work embodies a North American cultural studies approach to media studies (moving away from…

  5. Cultural Studies and Media Ecology: Meyrowitz's Medium Theory and Carey's Cultural Studies.

    Science.gov (United States)

    Flayhan, Donna P.

    2001-01-01

    Examines work of two communication and media studies scholars, Joshua Meyrowitz and James Carey. Suggests their studies represent media ecology with analyses of the dynamic interaction between communication, consciousness, and culture. Highlights how their work embodies a North American cultural studies approach to media studies (moving away from…

  6. Pure and carbon-doped boron phosphide (6,0) zigzag nanotube: A computational NMR study

    Energy Technology Data Exchange (ETDEWEB)

    Arshadi, S., E-mail: sattar_arshadi@yahoo.com [Department of Chemistry, Payame Noor University, 19395-4697, I.R. of Iran (Iran, Islamic Republic of); Bekhradnia, A.R., E-mail: abekhradnia@gmail.com [Pharmaceutical Sciences Research Center, Department of Medicinal Chemistry, Mazandaran University of Medical Sciences, Sari (Iran, Islamic Republic of); Department of Chemistry and Molecular Biology, Gothenburg University, Gothenburg (Sweden); Alipour, F.; Abedini, S. [Department of Chemistry, Payame Noor University, 19395-4697, I.R. of Iran (Iran, Islamic Republic of)

    2015-11-15

    Calculations were performed for investigation of the properties of the electronic structure of Carbon- Doped Boron Phosphide Nanotube (CDBPNT). Pristine and three models of C-doped structures of (6,0) zigzag BPNT were studied at density functional theory (DFT) in combination with 6-311G* basis set using Gaussian package of program. The calculated parameters reveal that various {sup 11}B and {sup 31}P nuclei are divided into some layers with equivalent electrostatic properties. The electronic structure properties are highly influenced by replacement of {sup 11}B and {sup 31}P atoms by {sup 12}C atoms in pristine model. Furthermore, the HOMO−LUMO gap energy for suggested doped models (I), (II) and (III) were lower than pure BPNT pristine systems. The dipole moment values of models (II) and (III) were decreased to 1.788 and 1.789, respectively while the dipole moments of model (I) were enhanced to 4.373, in compare to pure pristine one (2.586). The magnitude of changes in Chemical Shielding (CS) tensor parameters revealed that the electron density at the site of {sup 31}P was higher than that at the site of {sup 11}B due to carbon doping.

  7. Numerical study of thermally stratified shear flows at the interface between porous and pure-fluid layers

    Science.gov (United States)

    Papalexandris, Miltiadis V.; Antoniadis, Panagiotis D.

    2015-11-01

    In this talk we are concerned with thermally stratified flows at the interface between superposed porous and pure-fluid layers. In our study we employ a thermo-mechanical model for the flows of interest that was recently developed by our team. According to this model, both the fluid and the solid matrix are treated as two separate and identifiable continua that are in thermal non-equilibrium with each other. This allows for the derivation of a single set of equations that are simultaneously valid both in the porous and pure-fluid regions. First, we briefly present the basic steps of the derivation of the mathematical model and describe an algorithm for its numerical treatment. Then, we present and discuss numerical results for transient shear flows in the domains of interest, under both stable and unstable thermal stratification. Emphasis is placed on the effects of buoyancy to the evolution of the flow structures at the interface and on the mechanisms that induce thermal non-equilibrium inside the porous medium. This work is supported by the National Fund for Scientific Research (FNRS), Belgium.

  8. Pragmatics Study of Politeness and Cultural Difference

    Institute of Scientific and Technical Information of China (English)

    齐岩

    2008-01-01

    This paper aims at study the politeness in the pragmatic framework and reviews the main studies of politeness by western and Chinese scholars.Meanwhile,the writer tries to reveal the cultural difference existing in politeness by comparative study of western and Chinese language.

  9. Comparative pharmacokinetic study of mangiferin after oral administration of pure mangiferin and US patented polyherbal formulation to rats.

    Science.gov (United States)

    Kammalla, Ananth Kumar; Ramasamy, Mohan Kumar; Inampudi, Jyothi; Dubey, Govind Prasad; Agrawal, Aruna; Kaliappan, Ilango

    2015-04-01

    The US patented polyherbal formulation for the prevention and management of type II diabetes and its vascular complications was used for the present study. The xanthone glycoside mangiferin is one of the major effector constituents in the Salacia species with potential anti-diabetic activity. The pharmacokinetic differences of mangiferin following oral administration of pure mangiferin and polyherbal formulation containing Salacia species were studied with approximately the same dose 30 mg/kg mangiferin and its distribution among the major tissue in Wistar rats. Plasma samples were collected at different time points (15, 30, 60, 120, 180, 240, 360, 480, 600, 1,440, 2,160, and 2880 min) and subsequently analyzed using a validated simple and rapid LC-MS method. Plasma concentration versus time profiles were explored by non-compartmental analysis. Mangiferin plasma exposure was significantly increased when administered from formulation compared to the standard mangiferin. Mangiferin resided significantly longer in the body (last mean residence time (MRTlast)) when given in the form of the formulation (3.65 h). Cmax values of formulation (44.16 μg/mL) administration were elevated when compared to equivalent dose of the pure mangiferin (15.23 μg/mL). Tissue distribution study of mangiferin from polyherbal formulation was also studied. In conclusion, the exposure of mangiferin is enhanced after formulation and administration and could result in superior efficacy of polyherbal formulation when compared to an equivalent dose of mangiferin. The results indicate that the reason which delays the elimination of mangiferin and enhances its bioavailability might the interactions of the some other constituents present in the polyherbal formulation. Distribution study results indicate that mangiferin was extensively bound to the various tissues like the small intestine, heart, kidney, spleen, and liver except brain tissue.

  10. Types of cross-cultural studies in cross-cultural psychology

    NARCIS (Netherlands)

    van de Vijver, F.J.R.; Lonner, W.J.; Dinnel, D.L.; Hayes, S.A.; Sattler, D.N.

    2003-01-01

    From a methodological perspective cross-cultural studies in psychology differ in three dimensions. First, cross-cultural psychological studies can be exploratory or test specific hypotheses. Second, some cross-cultural studies compare countries or ethnic groups while other cross-cultural studies

  11. Culture and IS: A criticism of predefined cultural archetypes studies

    OpenAIRE

    2008-01-01

    Although there are many different models of national culture, most IS research has tended to rely almost solely on Hofstede’s cultural model (Keil et al., 2000; Straub, 1994; Tan et al., 1995; Watson et al., 1994; Myers and Tan, 2002; Kirkman et al. 2006).). This is perhaps not surprising, given that Hofstede’s typology of culture has been one of the most popular in many different fields of management (Myers and Tan, 2002). Although, this paper focuses on Hofstede’s model of national culture,...

  12. Civilisation/Cultural Studies in Grenoble.

    Science.gov (United States)

    Revauger, Jean-Paul

    1993-01-01

    The professional core of civilization/cultural studies (CCS) lies in the study of the relationship between context and discourse. Here, the necessity for the perspective of the outsider, combined with linguistic competence, creates the possibility of a distinctive disciplinary basis for CCS. (Author)

  13. Cultural Studies and Sociology of Culture in Germany: Relations and Interrelations

    Directory of Open Access Journals (Sweden)

    Udo Göttlich

    2013-06-01

    Full Text Available Over the last three decades, attitudes towards cultural studies in Germany have developed within contexts of contact and conflict with a variety of disciplines, e.g. ethnology, anthropology, sociology, as well as the sociology of culture, liter-ary studies and Kulturwissenschaft(en. On the one hand there is a strong academ-ic interest in how cultural studies perceives and analyzes media culture, popular culture and everyday life. On the other hand boundaries with humanities and so-cial science remain, which leads to criticism and conflicts with cultural studies and its achievements.I will discuss some of the problems concerning the perception and reception of cultural studies among representatives of Kulturwissenschaft(en and sociology of culture. Furthermore I will draw on the role of cultural studies in thematizing cul-tural change and conflicts, and its ability to do so in a way that shows the im-portance of culture and politics.

  14. A comparative study between transport and criticality safety indexes for fissile uranium nuclearly pure

    Energy Technology Data Exchange (ETDEWEB)

    Moraes da Silva, T. de; Sordi, G.M.A.A. [Instituto de Pesquisas Energeticas e Nucleares, IPEN/CNEN (Brazil)]. e-mail: tmsilva@ipen.br

    2006-07-01

    The international and national standards determine that during the transport of radioactive materials the package to be sent should be identified by labels of risks specifying content, activity and the transport index. The result of the monitoring of the package to 1 meter identifies the transport index, TI, which represents the dose rate to 1 meter of this. The transport index is, by definition, a number that represents a gamma radiation that crosses the superficial layer the radioactive material of the package to 1 meter of distance. For the fissile radioactive material that is the one in which a neutron causes the division of the atom, the international standards specify criticality safety index CSI, which is related with the safe mass of the fissile element. In this work it was determined the respective safe mass for each considered enrichment for the compounds of uranium oxides UO{sub 2}, U{sub 3}O{sub 8} and U{sub 3}Si{sub 2}. In the study of CSI it was observed that the value 50 of the expression 50/N being N the number of packages be transported in subcriticality conditions it represents a fifth part of the safe mass of the element uranium or 9% of the smallest mass critical for a transport not under exclusive use. As conclusion of the accomplished study was observed that the transport index starting from 7% of enrichment doesn't present contribution and that criticality safety index is always greater than the transport index. Therefore what the standards demand to specify, the largest value between both indexes, was clearly identified in this study as being the criticality safety index. (Author)

  15. Numerical study of tree-level improved lattice gradient flows in pure Yang-Mills theory

    CERN Document Server

    Kamata, Norihiko

    2016-01-01

    We study several types of tree-level improvement in the Yang-Mills gradient flow method in order to reduce the lattice discretization errors in line with a reference [Fodor et al., arXiv:1406.0827]. The tree-level $\\mathcal{O}(a^2)$ improvement can be achieved in a simple manner, where an appropriate weighted average is computed between two definitions of the action density $\\langle E(t)\\rangle$ measured at every flow time $t$. We further develop the idea of achieving the tree-level $\\mathcal{O}(a^4)$ improvement. For testing our proposal, we present numerical results of $\\langle E(t)\\rangle$ obtained on gauge configurations generated with the Wilson and Iwasaki gauge actions at three lattice spacings ($a\\approx 0.1, 0.07$ and 0.05 fm). Our results show that tree-level improved flows significantly eliminate the discretization corrections in the relatively small-$t$ regime. To demonstrate the feasibility of our proposal, we also study the scaling behavior of the dimensionless combinations of the $\\Lambda_{\\ove...

  16. Optical, thermal and magnetic studies of pure and cobalt chloride doped L-alanine cadmium chloride

    Science.gov (United States)

    Benila, B. S.; Bright, K. C.; Delphine, S. Mary; Shabu, R.

    2017-03-01

    Single crystals of L-alanine cadmium chloride (LACC) and cobalt chloride (Co2+) doped LACC have been grown by the slow evaporation solution growth technique. The grown crystals were subjected to various characterizations such as powder XRD, SXRD, FTIR, UV-vis, EDAX, TG/DTA, VSM, Dielectric and Second Harmonic Generation (SHG) measurements. The lattice parameters of the grown crystals were determined by single crystal X-ray analysis. EDAX analysis confirms the presence of Co2+ ion in the host material. The functional group and optical behavior of the crystals were identified from FTIR and UV-vis spectrum analysis. Electrical parameters such as dielectric constant, dielectric loss have been studied. The thermal stability of the compound was found out using TGA/DTA analysis. Second Harmonic Generation of the samples was confirmed by Kurtz-Perry powder technique. Magnetic properties of the crystals studied by VSM were also reported. The encouraging results show that the cobalt chloride doped LACC crystals have greater potential applications in optical devices.

  17. Influence of sludge retention time and temperature on the sludge removal in a submerged membrane bioreactor: comparative study between pure oxygen and air to supply aerobic conditions.

    Science.gov (United States)

    Rodríguez, F A; Leyva-Díaz, J C; Reboleiro-Rivas, P; González-López, J; Hontoria, E; Poyatos, J M

    2014-01-01

    Performance of a bench-scale wastewater treatment plant, which consisted of a membrane bioreactor, was monitored daily using pure oxygen and air to supply aerobic conditions with the aim of studying the increases of the aeration and sludge removal efficiencies and the effect of the temperature. The results showed the capacity of membrane bioreactor systems for removing organic matter. The alpha-factors of the aeration were determined for six different MLSS concentrations in order to understand the system working when pure oxygen and air were used to supply aerobic conditions in the system. Aeration efficiency was increased between 30.7 and 45.9% when pure oxygen was used in the operation conditions (a hydraulic retention time of 12 h and MLSS concentrations between 4,018 and 11,192 mg/L). Sludge removal efficiency increased incrementally, from 0.2 to 1.5% when pure oxygen was used at low sludge retention time and from 1.5% to 15.4% at medium sludge retention time when temperature conditions were lower than 20°C. Moreover, the difference between calculated and experimental sludge retention time was lesser when pure oxygen was used to provide aerobic conditions, so the influence of the temperature decreased when the pure oxygen was used. These results showed the convenience of using pure oxygen due to the improvement in the performance of the system.

  18. Numerical study of tree-level improved lattice gradient flows in pure Yang-Mills theory

    Science.gov (United States)

    Kamata, Norihiko; Sasaki, Shoichi

    2017-03-01

    We study several types of tree-level improvement in the Yang-Mills gradient flow method in order to reduce the lattice discretization errors in line with Fodor et al. [J. High Energy Phys. 09 (2014) 018., 10.1007/JHEP09(2014)018]. The tree-level O (a2) improvement can be achieved in a simple manner, where an appropriate weighted average is computed between the plaquette and clover-leaf definitions of the action density ⟨E (t )⟩ measured at every flow time t . We further develop the idea of achieving the tree-level O (a4) improvement within a usage of actions consisting of the 1 ×1 plaquette and 1 ×2 planar loop for both the flow and gauge actions. For testing our proposal, we present numerical results for ⟨E (t )⟩ obtained on gauge configurations generated with the Wilson and Iwasaki gauge actions at three lattice spacings (a ≈0.1 ,0.07 , and 0.05 fm). Our results show that tree-level improved flows significantly eliminate the discretization corrections on t2⟨E (t )⟩ in the relatively small-t regime for up to t ≳a2 . To demonstrate the feasibility of our tree-level improvement proposal, we also study the scaling behavior of the dimensionless combinations of the ΛMS ¯ parameter and the new reference scale tX, which is defined through tX2⟨E (tX)⟩=X for the smaller X , e.g., X =0.15 . It is found that √{t0.15 }ΛMS ¯ shows a nearly perfect scaling behavior as a function of a2 regardless of the types of gauge action and flow, after tree-level improvement is achieved up to O (a4) . Further detailed study of the scaling behavior exposes the presence of the remnant O (g2 na2) corrections, which are beyond the tree level. Although our proposal is not enough to eliminate all O (a2) effects, we show that the O (g2 na2) corrections can be well under control even by the simplest tree-level O (a2) improved flow.

  19. Turning the hands of time again: a purely confirmatory replication study and a Bayesian analysis.

    Science.gov (United States)

    Wagenmakers, Eric-Jan; Beek, Titia F; Rotteveel, Mark; Gierholz, Alex; Matzke, Dora; Steingroever, Helen; Ly, Alexander; Verhagen, Josine; Selker, Ravi; Sasiadek, Adam; Gronau, Quentin F; Love, Jonathon; Pinto, Yair

    2015-01-01

    In a series of four experiments, Topolinski and Sparenberg (2012) found support for the conjecture that clockwise movements induce psychological states of temporal progression and an orientation toward the future and novelty. Here we report the results of a preregistered replication attempt of Experiment 2 from Topolinski and Sparenberg (2012). Participants turned kitchen rolls either clockwise or counterclockwise while answering items from a questionnaire assessing openness to experience. Data from 102 participants showed that the effect went slightly in the direction opposite to that predicted by Topolinski and Sparenberg (2012), and a preregistered Bayes factor hypothesis test revealed that the data were 10.76 times more likely under the null hypothesis than under the alternative hypothesis. Our findings illustrate the theoretical importance and practical advantages of preregistered Bayes factor replication studies, both for psychological science and for empirical work in general.

  20. Turning the Hands of Time Again: A Purely Confirmatory Replication Study and a Bayesian Analysis

    Directory of Open Access Journals (Sweden)

    Eric-Jan eWagenmakers

    2015-04-01

    Full Text Available In a series of four experiments, Topolinski and Sparenberg (2012; TS found support for the conjecture that clockwise movements induce psychological states of temporal progression and an orientation toward the future and novelty. Here we report the results of a preregistered replication attempt of Experiment 2 from TS. Participants turned kitchen rolls either clockwise or counterclockwise while answering items from a questionnaire assessing openness to experience. Data from 102 participants showed that the effect went slightly in the direction opposite to that predicted by TS, and a preregistered Bayes factor hypothesis test revealed that the data were 10.76 times more likely under the null hypothesis than under the alternative hypothesis. Our findings illustrate the theoretical importance and practical advantages of preregistered Bayes factor replication studies, both for psychological science and for empirical work in general.

  1. Study of lubrication behavior of pure water for hydrophobic friction pair

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    The perfluorooctyltrichlorosilane molecular layer was self-assembled on glass plate. The tribological properties of the molecular layer in water were studied with the method of ball on disk. An interesting phenomenon was found that low friction coefficients of 0.02―0.08 were obtained when the friction pair was lubricated with only a water droplet. Whereas, when the friction pair was encircled with large amount of water or fully immersed in water, the friction coefficient was higher than that under a droplet lubrication. A mechanism of water droplet lubrication was proposed that the surface tension caused by the solid-liquid-air three-phase interface makes water molecules enter into the contact zone, which separates the two friction surfaces and provides a low friction coefficient. However, water film can hardly form when more water encircles the friction pair, due to the attraction between water molecules.

  2. Studies on gas sensing performance of pure and modified barium strontium titanate thick film resistors

    Indian Academy of Sciences (India)

    G H Jain; L A Patil; P P Patil; U P Mulik; K R Patil

    2007-02-01

    Barium strontium titanate ((Ba0.87Sr0.13)TiO3–BST) ceramic powder was prepared by mechanochemical process. The thick films of different thicknesses of BST were prepared by screen-printing technique and gas-sensing performance of these films was tested for various gases. The films showed highest response and selectivity to ammonia gas. The effect of film thickness on gas response was also studied. As prepared BST thick films were surface modified by dipping them into an aqueous solution of titanium chloride (TiCl3) for different intervals of time. Surface modification shifted response to H2S gas suppressing the responses to ammonia and other gases. The surface modification, using dipping process, altered the adsorbate–adsorbent interactions, which gave the unusual sensitivity and selectivity effect. Sensitivity, selectivity, thermal stability, response and recovery time of the sensor were measured and presented.

  3. Numerical study of large-N phase transition of smeared Wilson loops in 4D pure YM theory

    CERN Document Server

    Lohmayer, Robert

    2011-01-01

    In Euclidean four-dimensional SU(N) pure gauge theory, eigenvalue distributions of Wilson loop parallel transport matrices around closed spacetime curves show non-analytic behavior (a 'large-N phase transition') at a critical size of the curve. We focus mainly on an observable composed of traces of the Wilson loop operator in all totally antisymmetric representations, which is regularized with the help of smearing. By studying sequences of square Wilson loops on a hypercubic lattice with standard Wilson action, it is shown that this observable has a nontrivial continuum limit as a function of the physical size of the loop. We furthermore present (preliminary) numerical results confirming that, for large N, the N dependence in the critical regime is governed by the universal exponents 1/2 and 3/4 as expected (Burgers universality).

  4. Dielectric response of pure and doped-GaSe crystals studied by an indigenously developed broadband THz-TDS system

    Science.gov (United States)

    Das, Amit C.; Bhattacharya, S.; Mandal, K. C.; Mondal, S.; Jewariya, M.; Ozaki, T.; Bhaktha, S. N. B.; Datta, P. K.

    2016-04-01

    Publisher's Note: This paper, originally published on 12 July 2016, was replaced with a corrected/revised version on 26 July 2016. If you downloaded the original PDF but are unable to access the revision, please contact SPIE Digital Library Customer Service for assistance. We have developed a terahertz time domain spectroscopy system (THz TDS). For THz generation, optical rectification process and for detection, electro-optic sampling processes are used. Identical cut ZnTe crystals are used for both generation and detection of THz radiation.This spectroscopy system can be used for the noninvasive and contactless electrical and optical characterizations of various samples. In this work spectroscopic measurements of pure, Chromium and Indium doped GaSe crystals within 0.4 THz to 3 THz range are taken by the developed set-up to study the dielectric response of the samples.

  5. Effect of impurities and electrolyte thickness on degradation of pure magnesium: A finite element study

    Energy Technology Data Exchange (ETDEWEB)

    Montoya, R., E-mail: rodrigo.montoya@cenim.csic.es [Centro Nacional de Investigaciones Metalurgicas, CENIM, CSIC, Avda. Gregorio del Amo 8, 28040 Madrid (Spain); Departamento de Matematicas, Facultad de Quimica, Universidad Nacional Autonoma de Mexico, UNAM, Ciudad Universitaria, 04510 Mexico D.F. (Mexico); Departamento de Ingenieria Metalurgica, Facultad de Quimica, Universidad Nacional Autonoma de Mexico, UNAM, Ciudad Universitaria, 04510 Mexico D.F. Mexico (Mexico); Escudero, M.L., E-mail: escudero@cenim.csic.es [Centro Nacional de Investigaciones Metalurgicas, CENIM, CSIC, Avda. Gregorio del Amo 8, 28040 Madrid (Spain); Garcia-Alonso, M.C., E-mail: crisga@cenim.csic.es [Centro Nacional de Investigaciones Metalurgicas, CENIM, CSIC, Avda. Gregorio del Amo 8, 28040 Madrid (Spain)

    2011-12-15

    Highlights: Black-Right-Pointing-Pointer Degradation of Mg due to the presence of impurities by finite element method. Black-Right-Pointing-Pointer A thin film of electrolyte causes galvanic corrosion focused only close on impurities. Black-Right-Pointing-Pointer A thick layer of electrolyte provokes galvanic corrosion extended the whole surface. Black-Right-Pointing-Pointer A higher number of impurities causes galvanic corrosion on the Mg surface independently of electrolyte thickness. Black-Right-Pointing-Pointer The electrolyte thickness is an important variable that affects the in vivo degradation. - Abstract: The aim of this work is to study the degradation of magnesium due to the presence of impurities, by finite element method (FEM), when different thickness of physiological medium bathes the surface. The electrochemical experimental data obtained from polarization curves are used to model mathematically the corrosion process by solving the Laplace equation and the proper boundary conditions by means of FEM. The results show that when Mg is covered by a thin film of electrolyte, galvanic corrosion is focused only on the areas located really close to the cathodic sites, and far from the impurities, the Mg matrix remains near to its corrosion potential with a natural corrosion process. However, if the Mg matrix is completely covered by a thick layer of electrolyte the potentials obtained in the Mg surface far from the impurity are higher than its corrosion potential, so the Mg suffers more severe galvanic corrosion. On the other hand, when a higher number of impurities is considered, the Mg matrix is anodically polarized and it suffers severe galvanic corrosion, independently of h. The thickness of the electrolyte h must be considered as an important variable that affects the in vivo degradation.

  6. Effect of plane surface and wrinkly surface of pure Titanium on corrosion resistance and bacterium accumulation:an in vivo study

    Institute of Scientific and Technical Information of China (English)

    李笑梅; 汪大林; 郭天文; 齐文胜

    2004-01-01

    Objective: To study the effect of surface configuration on the corrosion resistance and bacterium accumulation of pure Titanium(Ti) when used in oral environment. Methods: Six edentulous volunteers with healthy oral mucosa participated in an in vivo study. Two kinds of pure Ti testing pieces with plane and wrinkly surface were fixed in the polished surface of upper complete dentures. After 6-month wearing, dynamic polarization curves were traced with electrochemical oral cavity was higher than that left in air, which meant corrosion resistance falling. Compared to plane one, Ecorr of wrinkly put a significant influence on amount of bacterium adhered on pure Titanium, but had no relation to species of bacterium. To the same patient, wrinkly samples collected more bacterium than plane ones, and exhibited G- coccus beside G+ coccus.Conclusion: From the perspective of corrosion behavior and benefit to periodontal tissue, wrinkly surface should not be adopted when pure Ti prosthesis is made, especially on connector part of denture.

  7. Study of Perceptions on Cultural Events’ Sustainability

    Directory of Open Access Journals (Sweden)

    Adina Letiţia Negruşa

    2016-12-01

    Full Text Available Cultural events and festivals can have a significant and important influence on the development of local communities. Their utilization of the endogenous resources of an area means that these events, most often, have a positive impact on the local economy. Quite consequentially, they may also extend the socio-cultural opportunities of local citizens. However, their utilization of time and space does raise concerns regarding environmental impact. Therefore, it is imperative that stakeholders study their net impact on a region. This present paper examines the economic, socio-cultural, and environmental impact of the Transilvania International Film Festival (TIFF. With a history of 15 editions, the festival gathers, year by year, an increasing number of people in Cluj-Napoca. The growth of the event has had unmistakable and important effects on the city. The purpose of the paper is to analyze resident participants’ perceptions on economic, socio-cultural, and environmental effects. To reach this goal, an exploratory and descriptive research was conducted. Both primary and secondary data were used in the analysis, the questionnaire being the main tool used for collecting data about participants’ perceptions. The results emphasize the positive effects at the socio-cultural level. The festival provides multiple possibilities to spend free time in a pleasant way, it sustains the development of cultural life, and it improves the educational and the cultural level of community. Moreover, the festival does not influence, in a negative manner, the moral principles of the society and it does not generate an increase in crime rate. From the economic point of view, the festival has the capacity to attract investments and additional revenues for the local government, it sustains the development of the city infrastructure, and it creates opportunities for residents to develop new economic activities. Lastly, from the environmental point of view, the

  8. Rapid identification and quantification of Campylobacter coli and Campylobacter jejuni by real-time PCR in pure cultures and in complex samples

    Science.gov (United States)

    2011-01-01

    Background Campylobacter spp., especially Campylobacter jejuni (C. jejuni) and Campylobacter coli (C. coli), are recognized as the leading human foodborne pathogens in developed countries. Livestock animals carrying Campylobacter pose an important risk for human contamination. Pigs are known to be frequently colonized with Campylobacter, especially C. coli, and to excrete high numbers of this pathogen in their faeces. Molecular tools, notably real-time PCR, provide an effective, rapid, and sensitive alternative to culture-based methods for the detection of C. coli and C. jejuni in various substrates. In order to serve as a diagnostic tool supporting Campylobacter epidemiology, we developed a quantitative real-time PCR method for species-specific detection and quantification of C. coli and C. jejuni directly in faecal, feed, and environmental samples. Results With a sensitivity of 10 genome copies and a linear range of seven to eight orders of magnitude, the C. coli and C. jejuni real-time PCR assays allowed a precise quantification of purified DNA from C. coli and C. jejuni. The assays were highly specific and showed a 6-log-linear dynamic range of quantification with a quantitative detection limit of approximately 2.5 × 102 CFU/g of faeces, 1.3 × 102 CFU/g of feed, and 1.0 × 103 CFU/m2 for the environmental samples. Compared to the results obtained by culture, both C. coli and C. jejuni real-time PCR assays exhibited a specificity of 96.2% with a kappa of 0.94 and 0.89 respectively. For faecal samples of experimentally infected pigs, the coefficients of correlation between the C. coli or C. jejuni real-time PCR assay and culture enumeration were R2 = 0.90 and R2 = 0.93 respectively. Conclusion The C. coli and C. jejuni real-time quantitative PCR assays developed in this study provide a method capable of directly detecting and quantifying C. coli and C. jejuni in faeces, feed, and environmental samples. These assays represent a new diagnostic tool for studying

  9. Development of secondary school student’s cultural competence during studies of History of Culture

    OpenAIRE

    2011-01-01

    Development of secondary school student’s cultural competence during studies of History of Culture The purpose of the thesis is to analyze the impact of the subject History of Culture on the development of secondary school students’ cultural competence. In addition the thesis analyzes the implemented methodology of the subject History of Culture and defines research results as suggestions for the improvement of the cultural competence. The thesis consists of three parts, where the...

  10. Potentiating effect of pure oxygen on the enhancement of respiration by ethylene in plant storage organs: a comparative study.

    Science.gov (United States)

    Theologis, A; Laties, G G

    1982-05-01

    A number of fruits and bulky storage organs were studied with respect to the effect of pure O(2) on the extent and time-course of the respiratory rise induced by ethylene. In one group, of which potato (Solanum tuberosum var. Russet) and carrot (Daucus carota) are examples, the response to ethylene in O(2) is much greater than in air. In a second group, of which avocado (Persea americana Mill. var. Hass) and banana (Musa cavendishii Lambert var. Valery) are examples, air and O(2) are equally effective. When O(2)-responsive organs are peeled, air and O(2) synergize the ethylene response to the same extent in parsnip (Pastinaca sativa), whereas O(2) is more stimulatory than air in carrots. In the latter instance, carrot flesh is considered to contribute significantly to diffusion resistance. The release of CO(2), an ethylene antagonist, is recognized as another element in the response to peeling.The potentiating effect of O(2) is considered to be primarily on ethylene action in the development of the respiratory rise rather than on the respiration process per se. On the assumption that diffusion controls O(2) movement into bulky organs and the peel represents the major diffusion barrier, simple calculations indicate that the O(2) concentration in untreated organs in air readily sustains respiration. Furthermore, in ethylene-treated organs in pure O(2), the internal O(2) concentration is more than enough to maintain the high respiration rates. Skin conductivity to O(2) is the fundamental parameter differentiating O(2)-responsive from O(2)-nonresponsive fruits and bulky storage organs. The large preceding the earliest response to ethylene, as well as the magnitude of the ethylene-induced respiratory rise, is also controlled by permeability characteristics of the peel.

  11. World Languages and Cultures Feasibility Study

    Science.gov (United States)

    Sklarz, David P.

    2013-01-01

    The purpose of the study was to conduct a comprehensive review of the research and best practices of exemplary elementary school World Language and Culture Programs. Specifically, (1) to assess the degree to which various program designs had most closely met the measurable goals of the American Council of Teachers of a Foreign Language, commonly…

  12. Studies in Philippine Languages and Cultures.

    Science.gov (United States)

    Brainard, Sherri, Ed.

    2001-01-01

    This issue of Studies in Philippine Languages and Cultures contains the following articles: "Functions of Locatives in Northern Subanen Expository and Hortatory Discourse" (Josephine Sanicas-Daguman); "Functions of Demonstratives in Sama Bangingi' Expository Discourse" (John Blakely); "A Brief Look at Sinama Basic Verbs…

  13. Repetitions: A Cross-Cultural Study.

    Science.gov (United States)

    Murata, Kumiko

    1995-01-01

    This study investigated how repetition is used in conversation among native speakers of British English, native speakers of Japanese, and Japanese speakers of English. Five interactional functions of repetition (interruption-orientated, solidarity, silence-avoidance, hesitation, and reformulation) were identified, as well as the cultural factors…

  14. Using activity theory to study cultural complexity in medical education

    NARCIS (Netherlands)

    Frambach, J.M.; Driessen, E.W.; Vleuten, C.P.M. van der

    2014-01-01

    There is a growing need for research on culture, cultural differences and cultural effects of globalization in medical education, but these are complex phenomena to investigate. Socio-cultural activity theory seems a useful framework to study cultural complexity, because it matches current views on

  15. Using activity theory to study cultural complexity in medical education

    NARCIS (Netherlands)

    Frambach, Janneke M; Driessen, Erik W; van der Vleuten, Cees P M

    There is a growing need for research on culture, cultural differences and cultural effects of globalization in medical education, but these are complex phenomena to investigate. Socio-cultural activity theory seems a useful framework to study cultural complexity, because it matches current views on

  16. Using activity theory to study cultural complexity in medical education

    NARCIS (Netherlands)

    Frambach, J.M.; Driessen, E.W.; Vleuten, C.P.M. van der

    2014-01-01

    There is a growing need for research on culture, cultural differences and cultural effects of globalization in medical education, but these are complex phenomena to investigate. Socio-cultural activity theory seems a useful framework to study cultural complexity, because it matches current views on

  17. (Emerging Discourses: Architecture and Cultural Studies

    Directory of Open Access Journals (Sweden)

    Sarah McGaughey

    2012-05-01

    Full Text Available Three recent works, Rosalind Galt’s Pretty, Anne Cheng’s Second Skin, and Daniel Purdy’s On the Ruins of Babel incorporate architectural history and architectural discourse into their analyses in ways that are new to their respective fields ranging from studies of film, gender, and race to intellectual history. Placing these three works in one essay allows for a detailed review of the ways in which each author employs architecture, at the same time as it reveals the benefits and challenges of incorporating architecture into cultural studies. The essay discusses the contributions of each work to their fields and also takes advantage of the different approaches to culture and architecture to explore the ways in which this relationship might continue to inform and generate productive studies.

  18. Tribological characterization of surface-treated commercially pure titanium for femoral heads in total hip replacement: a feasibility study.

    Science.gov (United States)

    Cotogno, G; Holzwarth, U; Franchi, M; Rivetti, S; Chiesa, R

    2006-12-01

    Most noncemented total hip replacements combine a titanium alloy stem, a CoCrMo femoral head and an ultra-high molecular weight polyethylene (UHMWPE) acetabular cup. In spite of its nickel content of up to 1% and the resulting biocompatibility issues in some clinical situations, the higher cost and some difficulties in machining, CoCrMo alloy is preferred to titanium alloys thanks to its outstanding tribological properties, higher hardness and elastic modulus. Nowadays most of the heads of hip prostheses use CoCrMo as bearing material. The present study investigates the effect of various surface treatments and combinations of treatments, such as electrochemical oxidation (anodization), laser surface melting and barrel polishing, on the tribological properties of commercially pure grade 2 titanium. The aim of the study was to characterize surface treatments capable of improving the tribological properties of titanium surface to the same extent as CoCrMo. The tribological properties were characterized by multidirectional pin-on-flat screening wear tests, using UHMWPE pins as bearing surface. The experiments showed the possibility of improving the wear resistance of titanium to the degree of CoCrMo. Although further efforts will be required to optimize the treatments studied, the results are encouraging enough to warrant pursuing this direction of investigation.

  19. Studies on suspension culture of virginia mallow

    Directory of Open Access Journals (Sweden)

    Anna Kasprzyk

    2014-04-01

    Full Text Available Virginia mallow (Sida hermaphrodita (L. Rusby belongs to the Malvaceae family. It is a very important industrial and energetic crop (Kasprzyk et al. 2013. In our studies, we used plant cell suspension cultures due to the fact that it is a useful tool to investigate biochemical, molecular and physiological aspects of many cellular functions (Dong et al. 2010. Virginia mallow seeds, obtained from Prof. Borkowska (University of Life Sciences in Lublin, Poland, were used in this investigation to obtain plants which were grown in sterile conditions in the Department of Plant Anatomy and Cytology, Maria Curie-Skłodowska University in Lublin, Poland. The seeds were surface sterilized and washed three times in sterile, distilled water. After 3 weeks of in vitro culture, young seedlings were used as a source of explants (to callus induction. Two types of explants were used to form callus culture: leaf and petiole. Callus tissues were then aseptically transferred to an Erlenmeyer flask with liquid medium and placed on an orbital shaker moving at 120 rpm. The observations of this suspense culture were conducted under light and confocal LSM microscopes. The authors observed that depending on the type of explants and composition of medium, callus tissue has varied in color and character of growth.

  20. A Comparative Numerical Study on the Performances and Vortical Patterns of Two Bioinspired Oscillatory Mechanisms: Undulating and Pure Heaving

    Directory of Open Access Journals (Sweden)

    Mohsen Ebrahimi

    2015-01-01

    Full Text Available The hydrodynamics and energetics of bioinspired oscillating mechanisms have received significant attentions by engineers and biologists to develop the underwater and air vehicles. Undulating and pure heaving (or plunging motions are two significant mechanisms which are utilized in nature to provide propulsive, maneuvering, and stabilization forces. This study aims to elucidate and compare the propulsive vortical signature and performance of these two important natural mechanisms through a systematic numerical study. Navier-Stokes equations are solved, by a pressure-based finite volume method solver, in an arbitrary Lagrangian-Eulerian (ALE framework domain containing a 2D NACA0012 foil moving with prescribed kinematics. Some of the important findings are (1 the thrust production of the heaving foil begins at lower St and has a greater growing slope with respect to the St; (2 the undulating mechanism has some limitations to produce high thrust forces; (3 the undulating foil shows a lower power consumption and higher efficiency; (4 changing the Reynolds number (Re in a constant St affects the performance of the oscillations; and (5 there is a distinguishable appearance of leading edge vortices in the wake of the heaving foil without observable ones in the wake of the undulating foil, especially at higher St.

  1. Study of transient photocurrents in nominally pure LiNbO{sub 3} depending on the reduction

    Energy Technology Data Exchange (ETDEWEB)

    Buescher, Andreas; Bruening, Hauke; Imlau, Mirco [Department of Physics, University of Osnabrueck, Barbarastr. 7, D-49069 Osnabrueck (Germany)

    2011-07-01

    In thermally reduced, nominally pure LiNbO{sub 3} the steady-state absorption is characterized by a broad absorption band at {lambda}=500 nm due to the presence of stable (Nb{sub Li}{sup 4+}:Nb{sub Nb}{sup 4+})-bipolarons. Upon illumination with intense pulse laser light in the blue green spectral range ({tau}= 8 ns, {lambda}=532 nm) these bipolarons can be dissociated into metastable small free (Nb{sub Nb}{sup 4+})-polarons and small bound (Nb{sub Li}{sup 4+})-polarons. This mechanism is responsible for the strong photochromic properties of this material. Furthermore, but less often studied, these polarons contribute to the conductivity comparable to the features of extrinsic Fe-doping. Like the Fe{sub Li} center does in iron-doped LiNbO{sub 3}. We present first results of measurements to detect the transient photocurrents associated to bound polarons using a fast operational amplifier. Our study highlights the impact of the number density of optically generated small polarons, which can be controlled by the reduction pre-treatment. We discuss the results in the framework of optical and electrical properties of small polarons in LiNbO{sub 3}. The number density of the excited polarons after illumination can be influenced by the degree of reduction and therefor the overall concentration of bipolarons in the material.

  2. A Comparative Numerical Study on the Performances and Vortical Patterns of Two Bioinspired Oscillatory Mechanisms: Undulating and Pure Heaving.

    Science.gov (United States)

    Ebrahimi, Mohsen; Abbaspour, Madjid

    2015-01-01

    The hydrodynamics and energetics of bioinspired oscillating mechanisms have received significant attentions by engineers and biologists to develop the underwater and air vehicles. Undulating and pure heaving (or plunging) motions are two significant mechanisms which are utilized in nature to provide propulsive, maneuvering, and stabilization forces. This study aims to elucidate and compare the propulsive vortical signature and performance of these two important natural mechanisms through a systematic numerical study. Navier-Stokes equations are solved, by a pressure-based finite volume method solver, in an arbitrary Lagrangian-Eulerian (ALE) framework domain containing a 2D NACA0012 foil moving with prescribed kinematics. Some of the important findings are (1) the thrust production of the heaving foil begins at lower St and has a greater growing slope with respect to the St; (2) the undulating mechanism has some limitations to produce high thrust forces; (3) the undulating foil shows a lower power consumption and higher efficiency; (4) changing the Reynolds number (Re) in a constant St affects the performance of the oscillations; and (5) there is a distinguishable appearance of leading edge vortices in the wake of the heaving foil without observable ones in the wake of the undulating foil, especially at higher St.

  3. Environmental Controls on Hopanoid Distributions: Field and Culture Studies

    Science.gov (United States)

    Albrecht, H. L.; Freeman, K. H.; Macalady, J. L.

    2008-12-01

    Hopanoid biomarkers play an important role in reconstructions of the earth's past biogeochemistry and evolution. Despite their excellent preservation potential and utility for microbial paleontology, relatively little of the earth's modern microbial biosphere has been explored with respect to hopanoid production. Due to the fact that hopanoids are used by bacteria to tune the physical properties of their cell membranes, geochemical gradients in nature may be associated with systematic changes in hopanoid diversity and abundances. Bacteriohopanpolyols (BHPs) are produced by both sulfur-oxidizing and sulfur-reducing bacteria. We hypothesize that BHPs are important for microbial adaptation to pH and sulfide concentrations in sulfur-oxidizing microbial communities. We investigated this hypothesis using both pure laboratory cultures and with samples of natural biofilms collected across oxygen and sulfide concentration gradients in the field. Samples were collected from the sulfidic Frasassi cave system, Italy, where the geomicrobiology of abundant sulfur cycling biofilms has previously been studied in detail (Macalady et al. 2007, Macalady et al. 2008). Culturing experiments were performed with two sulfur-oxidizing bacteria, an Acidithiobacillus sp. isolated from extremely acidic (pH 0-1) snotittes, and Beggiatoa alba, a neutrophilic sulfur oxidizer with close relatives in the Frasassi biofilms. Cultures were grown at range of pH and sulfide concentrations, harvested, freeze-dried and analyzed by atmospheric pressure chemical ionization liquid chromatography/mass spectrometry (APCI- LC/MS). We observed six different BHPs for the Acidithiobacillus sp. including one novel structure. Acidithiobacillus BHPs change in response to changes in the pH of the growth medium, becoming more polar and more abundant at lower pH. These observations are consistent with the production of less fluid, more polar membranes to counteract proton leakage into cells as pH decreases. Macalady, J

  4. PHYTOCHEMICAL STUDY OF CELL CULTURE JATROPHA CURCAS

    OpenAIRE

    KOMAR RUSLAN; ARTRI; ELFAHMI

    2011-01-01

    Jatropha curcas belongs to the Euphorbiaceae family which has potential economically. This plant has been reported to contain toxic compounds such as curcin and phorbol ester and its derivatives. These compounds may become a problem if J. curcas will be explored as a source of biofuel. In order to provide safety plants, the research on the study of phytochemical and initiation of cell and organ culture have been carried out. J curcas which has been collected from different regions in Indonesi...

  5. [The study of the pure tone audiometry characteristics and curative effect in sudden hearing loss patients with hypertension].

    Science.gov (United States)

    Li, Qian; Wang, Dayong; Wang, Hongyang; Lan, Lan; Han, Bing; Qi, Yue; Guan, Jing; Yin, Zifang; Wu, Ziming; Wang, Qiuju

    2014-04-01

    To investigate the pure tone audiometry characteristics and curative effect in sudden hearing loss patients with hypertension. One hundred and fifty-seven inpatients (168 ears) with hypertension suffered from sudden hearing loss were included in this study. We retrospectively analyzed the audiological index of these patients by comparing the pure tone audiometry (PTA) among patients in the aspects of gender, age, affected side, duration of hypertension, with or without inducement, concomitant symptoms and other combined diseases. The hearing threshold at different frequency was also compared, as well as the curative effect among patients with diverse audiological characteristics. Of the contemporaneous sudden hearing loss patients (874 cases), the prevalence of hypertension was 17.96%, where the male ones accounted for. 28.69% (103/359) and the female ones accounted for 19.42% (54/278) respectively with statistically significant difference between genders (P hearing threshold in PTA were increasingly severe as the increasing impaired-frequency, and the difference of the degree of hearing impairment among these three types of frequencies was statistically significant (P hearing threshold means of each frequency had no significant difference among patients with various gender, age and Cardiovascular Risk Stratification (P > 0.05). The hearing threshold means of each frequency of unilateral hearing loss patients was significantly higher than that of bilateral hearing loss patients (P hearing threshold means at 125 Hz, 250 Hz, 500 Hz and 1 kHz showed significant difference among patients with different duration of hypertension (P sudden hearing loss in patients with hypertension was significantly lower than that in the sudden hearing loss patients without hypertension (19.64%, 61.57% respectively, P sudden hearing loss in hypertension patients was higher in male than in female, which rose with age and combined disease. The hearing threshold means at mid-frequency and

  6. Study of transport properties and conduction mechanism of pure and composite resorcinol formaldehyde aerogel doped with Co-ferrite

    Energy Technology Data Exchange (ETDEWEB)

    Attia, S.M., E-mail: saidattia2004@yahoo.com [Physics Department, Faculty of Science, Kafrelsheikh University, Kafr El-Sheikh (Egypt); Physics Department, College of Applied Sciences, Umm Al-Qura University, PB 7296, Makkah 21955 (Saudi Arabia); Sharshar, T. [Physics Department, Faculty of Science, Kafrelsheikh University, Kafr El-Sheikh (Egypt); Physics Department, Faculty of Science, Taif University, Taif, 888 Hawiya (Saudi Arabia); Abd-Elwahed, A.R. [Physics Department, Faculty of Science, Kafrelsheikh University, Kafr El-Sheikh (Egypt); Tawfik, A. [Physics Department, Faculty of Science, Tanta University, Tanta (Egypt)

    2013-08-20

    Highlights: • A novel composite RF aerogels with Co-ferrite were prepared by sol–gel process. • RF aerogels exhibit a semiconducting behavior. • The dielectric constant of RF aerogel is very low (4 times as that of air) and can be controlled by adding Co-ferrite. • Large overlapping polaron (OLP) was found to be the preferred conduction mechanism in these materials. -- Abstract: A series of resorcinol formaldehyde aerogels (RF aerogels) composite with nanoparticles of CoFe{sub 2}O{sub 4} have been prepared by sol–gel method. Four samples of pure RF aerogels were prepared at different concentrations of Na{sub 2}CO{sub 3} as catalyst (0.02, 0.025, 0.03, and 0.04 wt.%) and four samples of composite RF aerogels were prepared at different concentration of doped CoFe{sub 2}O{sub 4} (0.075, 0.1, 0.125, and 0.15 wt.%; Na{sub 2}CO{sub 3} concentration = 0.03 wt.%). DC electrical conductivity as a function of temperature was studied in the temperature range 25 °C–200 °C for all samples. AC electrical conductivity and dielectric properties were determined using RLC Bridge in the frequency range 100 Hz–1 MHz at different temperature (25–200 °C). The pore size of the samples was determined using positron annihilation lifetime spectroscopy (PALS). RF aerogels are found to exhibit a semiconducting behavior and characterized by two transition temperatures T{sub 1} and T{sub 2}. Also σ{sub DC} increases with increase of Co-ferrite contents. Pure RF aerogels posses a very low dielectric constant, where the lowest value of ε′ is ∼4 times as that of air. ε′ decreases with increase of frequency, and increases with increase of temperature. Large overlapping polaron (OLP) is found to be the preferred conduction mechanism in these materials. The results of PALS show that there are two types of pore size in these samples; the first ranges from 1.9 to 2.5 nm, while the second ranges from 3.2 to 5.3 nm.

  7. A Review on the Study of Ethnic Minorities’ Cultural Identity Influenced by Different Cultures

    Institute of Scientific and Technical Information of China (English)

    Zhao Dan; Liu Yi

    2015-01-01

    Due to the rich content of cultural iden ̄tity, the research related to this aspect involves many disciplines, including anthropology, sociolo ̄gy, psychology, philosophy, literature, religion and education,etc. Based on their own academic back ̄ground,scholars have done a lot of research on va ̄rious aspects of the cultural identity of ethnic mi ̄norities. This article classifies cultural identity in ̄fluenced by different cultures, and focuses on a study of the impact and role of different cultural forms on the ethnic minorities’ cultural identity. The influences on the cultural identity of ethnic mi ̄norities include the following.

  8. Adsorption of Toluene and Paraxylene from Aqueous Solution Using Pure and Iron Oxide Impregnated Carbon Nanotubes: Kinetics and Isotherms Study

    Science.gov (United States)

    Abbas, Aamir; Ihsanullah; Al-Baghli, Nadhir A. H.

    2017-01-01

    Multiwall carbon nanotubes (CNTs) and iron oxide impregnated carbon nanotubes (CNTs-iron oxide) were investigated for the adsorption of hazardous toluene and paraxylene (p-xylene) from aqueous solution. Pure CNTs were impregnated with iron oxides nanoparticles using wet impregnation technique. Various characterization techniques including thermogravimetric analysis, scanning electron microscopy, elemental dispersion spectroscopy, X-ray diffraction, and nitrogen adsorption analysis were used to study the thermal degradation, surface morphology, purity, and surface area of the materials. Batch adsorption experiments show that iron oxide impregnated CNTs have higher degree of removal of p-xylene (i.e., 90%) compared with toluene (i.e., 70%), for soaking time 2 h, with pollutant initial concentration 100 ppm, at pH 6 and shaking speed of 200 rpm at 25°C. Pseudo-second-order model provides better fitting for the toluene and p-xylene adsorption. Langmuir and Freundlich isotherm models demonstrate good fitting for the adsorption data of toluene and p-xylene. PMID:28386208

  9. Theoretical study of the role of metallic contacts in probing transport features of pure and defected graphene nanoribbons.

    Science.gov (United States)

    La Magna, Antonino; Deretzis, Ioannis

    2011-03-18

    Understanding the roles of disorder and metal/graphene interface on the electronic and transport properties of graphene-based systems is crucial for a consistent analysis of the data deriving from experimental measurements. The present work is devoted to the detailed study of graphene nanoribbon systems by means of self-consistent quantum transport calculations. The computational formalism is based on a coupled Schrödinger/Poisson approach that respects both chemistry and electrostatics, applied to pure/defected graphene nanoribbons (ideally or end-contacted by various fcc metals). We theoretically characterize the formation of metal-graphene junctions as well as the effects of backscattering due to the presence of vacancies and impurities. Our results evidence that disorder can infer significant alterations on the conduction process, giving rise to mobility gaps in the conductance distribution. Moreover, we show the importance of metal-graphene coupling that gives rise to doping-related phenomena and a degradation of conductance quantization characteristics.

  10. Theoretical study of the role of metallic contacts in probing transport features of pure and defected graphene nanoribbons

    Directory of Open Access Journals (Sweden)

    La Magna Antonino

    2011-01-01

    Full Text Available Abstract Understanding the roles of disorder and metal/graphene interface on the electronic and transport properties of graphene-based systems is crucial for a consistent analysis of the data deriving from experimental measurements. The present work is devoted to the detailed study of graphene nanoribbon systems by means of self-consistent quantum transport calculations. The computational formalism is based on a coupled Schrödinger/Poisson approach that respects both chemistry and electrostatics, applied to pure/defected graphene nanoribbons (ideally or end-contacted by various fcc metals. We theoretically characterize the formation of metal-graphene junctions as well as the effects of backscattering due to the presence of vacancies and impurities. Our results evidence that disorder can infer significant alterations on the conduction process, giving rise to mobility gaps in the conductance distribution. Moreover, we show the importance of metal-graphene coupling that gives rise to doping-related phenomena and a degradation of conductance quantization characteristics.

  11. Pure dysarthria due to an insular infarction.

    Science.gov (United States)

    Hiraga, Akiyuki; Tanaka, Saiko; Kamitsukasa, Ikuo

    2010-06-01

    Cortical infarction presenting with pure dysarthria is rarely reported. Previous studies have reported pure dysarthria due to cortical stroke at the precentral gyrus or middle frontal gyrus. We report a 72-year-old man who developed pure dysarthria caused by an acute cortical infarction in the insular cortex. The role of the insula in language has been difficult to assess clinically because of the rarity of pure insular strokes. Our patient showed pure dysarthria without aphasia, indicating that pure dysarthria can be the sole manifestation of insular infarctions.

  12. PHYTOCHEMICAL STUDY OF CELL CULTURE JATROPHA CURCAS

    Directory of Open Access Journals (Sweden)

    KOMAR RUSLAN

    2011-01-01

    Full Text Available Jatropha curcas belongs to the Euphorbiaceae family which has potential economically. This plant has been reported to contain toxic compounds such as curcin and phorbol ester and its derivatives. These compounds may become a problem if J. curcas will be explored as a source of biofuel. In order to provide safety plants, the research on the study of phytochemical and initiation of cell and organ culture have been carried out. J curcas which has been collected from different regions in Indonesia showed to contain relatively the same profile of chemical contents. Dominant compounds that were detected by GCMS are hidrocarbon such as 2-heptenal, decadienal, hexsadecane, pentadecane, cyclooctane etc, fatty acid such as oktadecanoate acid, etthyl linoleate, ethyl stearate, heksadecanoate acid and steroid such as stigmasterol, fucosterol, sitosterol. No phorbol ester and its derivatives have been detected yet by the GCMS method. Callus and suspension cultures of J. curcas have been established to be used for further investigation.

  13. Pure animal phobia is more specific than other specific phobias: epidemiological evidence from the Zurich Study, the ZInEP and the PsyCoLaus.

    Science.gov (United States)

    Ajdacic-Gross, Vladeta; Rodgers, Stephanie; Müller, Mario; Hengartner, Michael P; Aleksandrowicz, Aleksandra; Kawohl, Wolfram; Heekeren, Karsten; Rössler, Wulf; Angst, Jules; Castelao, Enrique; Vandeleur, Caroline; Preisig, Martin

    2016-09-01

    Interest in subtypes of mental disorders is growing in parallel with continuing research progress in psychiatry. The aim of this study was to examine pure animal phobia in contrast to other specific phobias and a mixed subtype. Data from three representative Swiss community samples were analysed: PsyCoLaus (n = 3720), the ZInEP Epidemiology Survey (n = 1500) and the Zurich Study (n = 591). Pure animal phobia and mixed animal/other specific phobias consistently displayed a low age at onset of first symptoms (8-12 years) and clear preponderance of females (OR > 3). Meanwhile, other specific phobias started up to 10 years later and displayed almost a balanced sex ratio. Pure animal phobia showed no associations with any included risk factors and comorbid disorders, in contrast to numerous associations found in the mixed subtype and in other specific phobias. Across the whole range of epidemiological parameters examined in three different samples, pure animal phobia seems to represent a different entity compared to other specific phobias. The etiopathogenetic mechanisms and risk factors associated with pure animal phobias appear less clear than ever.

  14. Study Circles and Socio-cultural Animation

    Directory of Open Access Journals (Sweden)

    Vilma Malečkar

    2001-12-01

    Full Text Available Informal learning and participating in study circles is a way of applying the ideas of socio-cultural animation. It is based on the assumption that within a society there are mechanisms that institutions don't comprise and therefore don't fulfil various, often urgent needs deriving from everyday life and the community. What is going on here is identifying and solving burning problems; some of them have already become an integral part of the way of living in a community. Study circles as an informal phenomenon in Slovenia create new possibilities of social activities based on common learning and participating in a community.

  15. Production of NO and N(inf2)O by Pure Cultures of Nitrifying and Denitrifying Bacteria during Changes in Aeration

    NARCIS (Netherlands)

    Kester, R.A.; De Boer, W.; Laanbroek, H.J.

    1997-01-01

    Peak emissions of NO and N2O are often observed after wetting of soil, The reactions to sudden changes in the aeration of cultures of nitrifying and denitrifying bacteria with respect to NO and N2O emissions were compared to obtain more information about the microbiological aspects of peak emissions

  16. Penerapan Cultural Studies dan Aliran Filsafat dalam Desain Komunikasi Visual

    Directory of Open Access Journals (Sweden)

    Laura Christina Luzar

    2014-10-01

    Full Text Available Cultural studies is a diversity knowledge from different variety of perspectives, through the production of theory trying to intervene in political culture. Cultural studies explores culture as a practice purport in the context of social force. In this case, cultural studies is not only based on one point only, but also cultural studies tries to compose a variety of theoretical studies of other disciplines developed wider, so that covers a wide range of academic theories that already existed, including Marxism, Structuralism, Post-structuralism, and feminism. By eclectic method, cultural studies puts the positioning to all knowledges, including on its knowledge which integrates with culture, practice of signification, representation, discourse, authority, articulation, text, read, and consumption. Cultural studies could be described as a language game or formation of discourse associated with relation to power in signification practice of human life. In addition to cultural studies, there is also feminism theory participated in the concept of feminist cultural studies that reconstructs and transforms view of misperception between feminism and cultural studies. Feminism affects cultural studies, but not all feminism can be viewed as cultural studies, and not all cultural studies talks about gender. Both of cultural studies and feminism have substantive importance in relation to power, representation, pop-culture, subjectivity, identity and consumption. The theory of social construction is also has connectivity with cultural studies. Construction of reality is inseparable from mark, symbol, and language. Media are full of reality constructed for people to affect people as ethics persuasion in media do. 

  17. Density functional theory study of the interaction of H2 with pure and Ti-doped WO3 (002) surfaces

    Institute of Scientific and Technical Information of China (English)

    Hu Ming; Wang Wei-Dan; Zeng Peng; Zeng Jing; Qin Yu-Xiang

    2012-01-01

    Density functional theory (DFT) calculations are conducted to explore the interaction of H2 with pure and Tidoped WO3 (002) surfaces.Four top adsorption models of H2 on pure and Ti-doped WO3 (002) surfaces are investigated respectively,they are adsorption on bridging oxygen O1c,absorption on plane oxygen O2c,absorption on 5-fold Wsc (Ti),and absorption on 6-fold W6c.The most stable and H2 possible adsorption structure in the pure surface is H-end oriented to the surface plane oxygen O2c site,while the favourable adsorption sites for H2 in a Ti-doped surface is not only an O2c site but also a W6c site.The adsorption energy,the Fermi energy level EF,and the electronic population are investigated and the H2-sensing mechanism of a pure-doped WO3 (002) surface is revealed theoretically:the theoretical results are in good accordance with our existing experimental results.By comparing the above three terms,it is found that Ti doping can obviously enhance the adsorption of H2.It can be predicted that the method of Ti-doped into a WO3 thin film is an effective way to improve WO3 sensor sensitivity to H2 gas.

  18. Antimicrobial activity and second harmonic studies on organic non-centrosymmetric pure and doped ninhydrin single crystals

    Science.gov (United States)

    Prasanyaa, T.; Jayaramakrishnan, V.; Haris, M.

    2013-03-01

    In this paper, we report the successful growth of pure, Cu2+ ions and Cd2+ ions doped on ninhydrin single crystals by slow solvent evaporation technique. The presence of Cu2+ and Cd2+ ions in the specimen of ninhydrin single crystal has been determined by atomic absorption spectroscopy. The powder X-ray diffraction analysis was done to calculate the lattice parameters of the pure and doped crystals. The percentage of transmittance of the crystal was recorded using the UV-Vis Spectrophotometer. Thermal behaviors of the grown crystals have been examined by the thermal gravimetric/differential thermal analysis. The hardness of the grown crystals was assessed and the results show the minor variation in the hardness value for the pure and doped ninhydrin samples. The value of the work hardening coefficient n was found to be 2.0, 1.0 and 1.06 for pure, copper and cadmium doped ninhydrin crystals respectively. The second harmonic generation efficiency of Cd2+ and Cu2+ doped ninhydrin is 8.3 and 6.3 times greater than well known nonlinear crystal of potassium dihydrogen phosphate respectively. The antibacterial and antifungal activities of the title compound were performed by disk diffusion method against the standard bacteria Escherichia coli, Xanthomonas oryzae and against the fungus Aspergillis niger and Aspergillus flavus.

  19. Study of pure and Pb{sup 2+} ions doped CsI crystals under alpha particles excitations

    Energy Technology Data Exchange (ETDEWEB)

    Pereira, Maria da Conceicao Costa; Madi Filho, Tufic; Hamada, Margarida Mizue, E-mail: macoper@ipen.b [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)

    2011-07-01

    Scintillation crystals have been used in various fields, such as high energy physics, nuclear instrumentation, radiation measurements, medical imaging, nuclear tomography, astrophysics and other fields of science and engineering. For these applications, the development of good performance scintillation crystals is required. Scintillation crystals based on cesium iodide (CsI) matrix are matters with relatively low hygroscope, easy handling and low cost, characteristics that favor their use as radiation detectors. In this work, pure CsI crystal and lead doped CsI crystals were grown using the Bridgman vertical technique. The concentration of the lead doping element (Pb) was studied in the range of 10{sup -2} M to 5x10{sup -4} M. The distribution of the doping element in the crystalline volume was determined by flame atomic absorption. The CsI:Pb crystal with nominal concentration of 10{sup -3} M was cut into 14 slices of 6 mm. The results show a higher concentration at the top of the crystal with a decrease in the initial phase of growth. The dopant concentration of Pb showed good uniformity from the slice 2 to the slice 12: the region is, therefore, suitable for use as radiation detector. The luminescence emission of these crystals were measured. A predominant luminescence band near 450 nm and a single broad band around 320 nm were found with the addition of the Pb{sup 2+} ions. Analyses were carried out to evaluate the developed scintillators, concerning alpha particles. The resolution of 5.6% was obtained for the CsI:Pb 5x10{sup -4} M crystal, when excited with alpha particles from a {sup 241}Am source, with energy of 5.54 MeV. (author)

  20. Some studies on mechanical properties and microstructural characterization of automated TIG welding of thin commercially pure titanium sheets

    Energy Technology Data Exchange (ETDEWEB)

    Karpagaraj, A.; Siva shanmugam, N., E-mail: nsiva@nitt.edu; Sankaranarayanasamy, K.

    2015-07-29

    Gas Tungsten Arc Welding (GTAW) is a commonly used welding process for welding Titanium materials. Welding of titanium and its alloys poses several intricacies to the designer as they are prone to oxidation phenomenon. To overcome this contamination, a relatively new type of shielding arrangement is experimented. The proposed design and arrangement have been employed for joining commercially pure titanium sheets with variations in the GTAW process parameters namely the welding current and travel speed. Bead on plate (BoP) trials were conducted on thin sheets of 2 mm thickness by varying the process parameters. Subsequently, the macro structure images were captured. Based on these results, the process parameters are chosen for carrying out full penetration butt joints on 1.6 mm and 2 mm thick titanium sheets. The influences of these parameters of GTAW on the microstructure, mechanical properties and surface morphology at the fractured locations of the welded joints are examined. The microstructural properties of base metal, heat affected zone and fusion zone are analyzed through optical microscopy. The welded joints showed an ultimate tensile strength of about 383 MPa with 15.7% elongation. The hardness value at fusion zone and base metal are typically observed to be 191 and 153 HV-0.5, respectively. X-ray diffraction study is conducted to examine the chemical composition in the parent metal and fusion zone of the weld. Fractured surface is examined using Scanning Electron Microscopy which revealed dimple kind of rupture present at the fractured surfaces owing to insufficient or excessive heat with slight impurities that prevents the accomplishment of stronger micro-level weld integrity.

  1. Adsorption/desorption phenomena on pure and Teflon AF-coated titania surfaces studied by dynamic contact angle analysis.

    Science.gov (United States)

    Rupp, F; Axmann, D; Ziegler, C; Geis-Gerstorfer, J

    2002-12-15

    As a result of inflammatory processes, plaque formation on dental titanium implants often leads to clinically pathogenic situations. This special biofilm formation on (bio)materials in contact with saliva is initiated by ionic and protein interactions. In this interfacial process, albumin becomes a main constituent of dental pellicle. Interfacial reactions change the surface characteristics. They determine the following steps of macromolecular adsorption and bacterial adhesion. This work focuses on the dynamic contact angle analysis (DCA), which is a tool for online measurements of dynamic changes of wettability without disturbing the interface during detection. Repeatability of the DCA method has been assessed according to the Bland and Altman method. The kinetics and equilibrium data of shifts in the wetting tension hysteresis indicate ionic influences at the titanium/bovine serum albumin (BSA) interface: the Ca-mediated increase of the BSA adsorption on titanium and the adsorption maximum at the isoelectric point (IEP) of BSA. Ti was surface modified by Teflon AF polymeric coatings. The result of the assessment gives reason to consider Teflon AF as a reference material for DCA repeatability studies. This surface modification caused drastic changes in the dynamic interfacial reactions. Shifts in the wetting tensions during DCA adsorption-desorption experiments clearly demonstrated the partially irreversible adsorption of BSA on Teflon AF. In contrast, reversible adsorption behavior was detected on pure Ti surfaces. These findings strengthen the hypothesis that the analysis of dynamic changes in wetting tension and wetting tension hysteresis is a sensitive analytical method for the detection of dynamic interfacial changes at biomaterial/biosystem interfaces during the initial steps of biofilm formation.

  2. The Virginian's Cultural Clashes: A Study of Cultural Representation in Owen Wister's novel The Virginian

    OpenAIRE

    Aksnes, Karoline

    2012-01-01

    This thesis is a cultural study of Owen Wister's novel The Virginian. Its main focus is on the ways in which cultural differences between East and West are staged and acted out in the novel's depiction of a culturally diverse frontier environment in nineteenth century Wyoming, USA. Central in this study are the story's three main characters as they represent different cultural backgrounds, and, by their personal experiences with the Western environment as well as wi...

  3. On the Importance of Cultural Factors in Oral English Studying

    Institute of Scientific and Technical Information of China (English)

    Xiong Juan

    2016-01-01

    Language communication, in some way, is cultural communication, in order to have a better ability of communicating, students have to know and understand enough culture knowledge. This paper will analyze the relationship between language and culture, emphasize the importance of cultural factors in oral English studying.

  4. Karl Marx and the Study of Media and Culture Today

    OpenAIRE

    Fuchs, Christian

    2014-01-01

    The task of this paper discusses the role of Marx in analysing media, communica-tion and culture today. An analysis of three contemporary Cultural Studies works – Lawrence Grossberg’s monograph Cultural Studies in the Future Tense, John Hartley’s monograph Digital Futures for Cultural and Media Studies and Paul Smith’s edited volume The Renewal of Cultural Studies – shows that there is an agreement that the economy needs to be taken more into account by Cultural Studies, but disagreement on w...

  5. Sixth form pure mathematics

    CERN Document Server

    Plumpton, C

    1968-01-01

    Sixth Form Pure Mathematics, Volume 1, Second Edition, is the first of a series of volumes on Pure Mathematics and Theoretical Mechanics for Sixth Form students whose aim is entrance into British and Commonwealth Universities or Technical Colleges. A knowledge of Pure Mathematics up to G.C.E. O-level is assumed and the subject is developed by a concentric treatment in which each new topic is used to illustrate ideas already treated. The major topics of Algebra, Calculus, Coordinate Geometry, and Trigonometry are developed together. This volume covers most of the Pure Mathematics required for t

  6. Population Muscle Strength Predicts Olympic Medal Tallies: Evidence from 20 Countries in the PURE Prospective Cohort Study

    Science.gov (United States)

    Leong, Darryl P.; McKee, Martin; Yusuf, Salim

    2017-01-01

    Background National sporting achievement at the Olympic Games is important for national pride and prestige, and to promote participation in sport. Summer Olympic Games medal tallies have been associated with national wealth, and also social development and healthcare expenditure. It is uncertain however, how these socioeconomic factors translate into Olympic success. The objective of this study was therefore to examine the relationship between population muscle strength and Olympic medal tallies. Methods and Results This study of handgrip strength represents a cross-sectional analysis of the Prospective Urban Rural Epidemiology (PURE) study, which is an ongoing population cohort study of individuals from high-, middle-, and low-income countries. Within participating countries, households from both urban and rural communities were invited to participate using a sampling strategy intended to yield a sample that was representative of the community. Households were eligible if at least one member was aged 35–70 years and if they intended living at the same address for a further four years. A total of 152,610 participants from these households, located in 21 countries, were included in this analysis. Handgrip strength was measured using a Jamar dynanometer. Olympic medal tallies were made over the five most recent Summer Games. There was a significant positive association between national population grip strength (GS) and medal tally that persisted after adjustment for sex, age, height, average daily caloric intake and GDP (total and per capita). For every 1kg increase in population GS, the medal tally increased by 36% (95% CI 13–65%, p = 0.001) after adjustment. Among countries that won at least one medal over the four most recent Summer Olympic Games, there was a close linear relationship between adjusted GS and the natural logarithm of the per capita medal tally (adjusted r = 0.74, p = 0.002). Conclusions Population muscle strength may be an important determinant

  7. Cultural Studies und kritische Pädagogik

    Directory of Open Access Journals (Sweden)

    Rainer Winter

    2004-02-01

    Full Text Available Der Autor stellt die Cultural Studies als transdisziplinäre Forschungsrichtung vor, die in den 50er Jahren in Großbritannien begründet wurde. Diese orientieren sich an alltäglichen Lebenswelten, "Kultur" bezeichnet ein Netzwerk von gesellschaftlichen Strukturen, die durch Herrschaftsverhältnisse determiniert sind. Ziel ist es, diese Strukturen transparent und transformierbar zu machen. Hier nähert sich der Ansatz der kritischen Pädagogik, die davon ausgeht, dass die Bildungssysteme die bestehenden Herrschaftsverhältnisse nicht einfach hinnehmen, sondern als veränderbar determinieren müssen. Übertragen auf mediale Texte werden die RezipientInnen als Subjekte wahrgenommen, die die Bedeutung der Texte vor dem Hintergrund ihrer sozialen Bedingungen produzieren. Der Autor beleuchtet Ansätze der Cultural Studies und der kritischen Pädagogik und stellt fest, dass beide Forschungsrichtungen einander implizieren. Im Zeitalter des Neoliberalismus und der interaktiven Medien sei es erforderlich, Jugendliche zu handlungsfähigen Subjekten zu erziehen, damit eine gerechtere und demokratischere Gesellschaft möglich sei. Dies erfordere die Erkenntnis, dass gesellschaftliche Strukturen in Medien sichtbar werden, aber auch veränderbar sind.

  8. Fundamental study of green EUV lithography using natural polysaccharide for the use of pure water in developable process

    Science.gov (United States)

    Takei, Satoshi

    2016-10-01

    The eco-conscious lithography processes of using pure water instead of spin coating organic solvent and alkaline developer were described for extreme-ultraviolet and electron beam techniques of advanced photomask manufactural application. Natural polysaccharide was obtained by the esterification of the hydroxyl groups of the polysaccharide resulting in improved resolution and resist profiles after the purewater developing processes. The 100, 200, and, 300 nm line and space width, and straight profiles of polysaccharide-based resist material on hardmask underlayer were resolved at the doses of 30 μC/cm2. In addition to the superior resolution in the pure-water developing processes, the resist material containing the polysaccharide derivatives for these lithography showed good resist profiles and step filling performance on substrates.

  9. Comparative in vitro Study on Pure Metals (Fe, Mn, Mg, Zn and W)as Biodegradable Metals

    Institute of Scientific and Technical Information of China (English)

    J.Cheng; B.Liu; Y.H.Wu; Y.F.Zheng

    2013-01-01

    Five pure metals including Fe,Mn,Mg,Zn and W have been investigated on their corrosion behavior and in vitro biocompatibility by electrochemical measurement,static immersion test,contact angle measurement,cytotoxicity and hemocompatibility tests.It is found that the sequence of corrosion rate of five metals in Hank's solution from high to low is:Mg > Fe > Zn > Mn > W.Fe,Mg and W show no cytotoxicity to L929 and ECV304 cells,Mn induces significant cytotoxicity to both L929 and ECV304 cells,and Zn has almost no inhibition effect on the metabolic activities of ECV304 while largely reduces the cell viability of L929 cells.The hemolysis percentage of five pure metals is lower than 5% except for Mg and platelets adhered on Zn has been activated and pseudopodia-like structures can be observed while platelets on the other four metals keep normal.

  10. Pure Parsimony Xor Haplotyping

    CERN Document Server

    Bonizzoni, Paola; Dondi, Riccardo; Pirola, Yuri; Rizzi, Romeo

    2010-01-01

    The haplotype resolution from xor-genotype data has been recently formulated as a new model for genetic studies. The xor-genotype data is a cheaply obtainable type of data distinguishing heterozygous from homozygous sites without identifying the homozygous alleles. In this paper we propose a formulation based on a well-known model used in haplotype inference: pure parsimony. We exhibit exact solutions of the problem by providing polynomial time algorithms for some restricted cases and a fixed-parameter algorithm for the general case. These results are based on some interesting combinatorial properties of a graph representation of the solutions. Furthermore, we show that the problem has a polynomial time k-approximation, where k is the maximum number of xor-genotypes containing a given SNP. Finally, we propose a heuristic and produce an experimental analysis showing that it scales to real-world large instances taken from the HapMap project.

  11. Pure parsimony xor haplotyping.

    Science.gov (United States)

    Bonizzoni, Paola; Della Vedova, Gianluca; Dondi, Riccardo; Pirola, Yuri; Rizzi, Romeo

    2010-01-01

    The haplotype resolution from xor-genotype data has been recently formulated as a new model for genetic studies. The xor-genotype data is a cheaply obtainable type of data distinguishing heterozygous from homozygous sites without identifying the homozygous alleles. In this paper, we propose a formulation based on a well-known model used in haplotype inference: pure parsimony. We exhibit exact solutions of the problem by providing polynomial time algorithms for some restricted cases and a fixed-parameter algorithm for the general case. These results are based on some interesting combinatorial properties of a graph representation of the solutions. Furthermore, we show that the problem has a polynomial time k-approximation, where k is the maximum number of xor-genotypes containing a given single nucleotide polymorphisms (SNP). Finally, we propose a heuristic and produce an experimental analysis showing that it scales to real-world large instances taken from the HapMap project.

  12. Adsorption of small gas molecules on pure and Al-doped graphene sheet: a quantum mechanical study

    Indian Academy of Sciences (India)

    DHARMVEER SINGH; ASHEESH KUMAR; DEVESH KUMAR

    2017-10-01

    The interaction of small gas molecules (CCl$_4$, CH$_4$, NH$_3$, CO$_2$, N$_2$, CO, NO$_2$ CCl$_2$F$_2$, SO$_2$, CF$_4$, H$_2$) on pure and aluminium-doped graphene were investigated by using the density functional theory to explore their potential applications as sensors. It has been found that all gas molecules show much stronger adsorption on the Al-doped graphene than that of pure graphene (PG). The Al-doped graphene shows the highest adsorption energy with NO$_2$, NH$_3$ and CO$_2$ molecules, whereas the PG binds strongly with NO$_2$. Therefore, the strong interactions between the adsorbed gas molecules and the Al-doped graphene induce dramatic changes to graphene’s electronic properties. These results reveal that the sensitivity of graphene-based gas sensor could be drastically improved by introducing the appropriate dopant or defect. It also carried out the highest occupied molecular orbital–lowest unoccupied molecular orbital energy gap of the complex molecular structure that has been explored by M06/6-31++G$^{**}$ method. These results indicate that the energy gap fine tuning of the pure and Al-doped graphene can be affected through the binding of small gas molecules.

  13. How Culture Affects Female Inequality across Countries: An Empirical Study

    Science.gov (United States)

    Cheung, Hoi Yan; Chan, Alex W. H.

    2007-01-01

    Many studies have commented that culture has an influence on gender inequality. However, few studies have provided data that could be used to investigate how culture actually influences female inequality. One of the aims of this study is to investigate whether Hofstede's cultural dimensions have an impact on female inequality in education in terms…

  14. Cultural Studies Methodologies and Narrative Family Therapy: Therapeutic Conversations About Pop Culture.

    Science.gov (United States)

    Tilsen, Julie; Nylund, David

    2016-06-01

    Therapists recognize that popular media culture is an influential force that shapes identities and relationships in contemporary society. Indeed, people have serious relationships with the commodities and practices that emerge from pop culture. However, they often lack the conceptual and conversational resources to engage meaningfully with clients about pop culture's influence in their lives. Cultural studies is introduced as an interdisciplinary approach that provides frameworks for both theory and practice that position therapists and clients to critically examine the role of pop culture in their lives. Cultural studies and narrative therapy are discussed as praxis allies that share a populist political intention and counter-hegemonic discursive practices. The integration of cultural studies methodologies into narrative therapy practice with a parent and her teenage daughter is illustrated through a case vignette.

  15. Terahertz applications in cultural heritage: case studies

    Science.gov (United States)

    Giovannacci, D.; Martos-Levif, D.; Walker, G. C.; Menu, M.; Detalle, V.

    2013-11-01

    Terahertz (THz) spectroscopy and imaging is a non-destructive, non-contact, non-invasive technology emerging as a tool for the analysis of cultural heritage. THz Time Domain Spectroscopy (TDS) techniques have the ability to retrieve information from different layers within a stratified sample, that enable the identification of hidden sub-layers in the case of paints and mural paintings. In this paper, we present the THz TDS2 system developed in the European Commission's 7th Framework Program project CHARISMA [grant agreement no. 228330]. Bespoke single processing algorithms; including a deconvolution algorithm can be deployed to increase the resolution and the global performance of the system. The potential and impact of this work is demonstrated through two case studies of mural paintings, where the capability to reveal the stratigraphy of the artworks is demonstrated.

  16. Experimental Study and Finite Element Polycrystal Model Simulation of the Cold Rolling Textures in a Powder Metallurgy Processed Pure Aluminum Plate

    Institute of Scientific and Technical Information of China (English)

    Liqing CHEN; Naoyuki Kanetake

    2005-01-01

    Ingot metallurgy (IM) aluminum has long been the subject and attracted the attention of many metallurgists and textural researchers of materials. Due to the introduction of large amounts of ex situ interfaces, however, the textures in powder metallurgy (PM) processed aluminum has been rarely reported. In this article, a pure aluminum plate was prepared via PM route. The starting billet was first produced with uni-axially cold compaction and flat hot-extrusion and then followed by cold rolling processes. The hot-extruded and cold rolling deformation textures of the pure PM aluminum at 50%, 80% and 90% cold rolling reductions were studied by orientation distribution functions (ODFs) analysis. The finite element polycrystal model (FEPM) was finally utilized to simulate the cold rolling textural evolution at various stages of cold rolling. In FEPM simulation, the initial hot-extruded textures were taken into account as inputs. The results showed that typical β-fiber texture formed in pure PM aluminum with the cold rolling reduction increased till 80%, and there was not much change after excessive cold rolling deformation.Homogeneous slip is not the only deformation mode in PM processed pure aluminum plate at over 80% cold rolling reduction. The experimental results were qualitatively in good agreement with the simulated ones.

  17. In-Depth Cultural Studies in Multicultural Group

    Science.gov (United States)

    Silina-Jasjukevica, Gunta; Briška, Ilze

    2016-01-01

    There is much research and educational practices at all levels of education on how to deal with promoting acceptance and understanding between different cultures. A cultural study forms an important part of shaping intercultural understanding. "The aim" of the research is to analyze an innovative way of incorporating cultural studies in…

  18. Promoting Teacher Growth through Lesson Study: A Culturally Embedded Approach

    Science.gov (United States)

    Ebaeguin, Marlon

    2015-01-01

    Lesson Study has captured the attention of many international educators with its promise of improved student learning and sustained teacher growth. Lesson Study, however, has cultural underpinnings that a simple transference model overlooks. A culturally embedded approach attends to the existing cultural orientations and values of host schools.…

  19. Dilemmas of Cultural Mediation: A Case Study of Tourism

    OpenAIRE

    Ajtony Zsuzsanna

    2015-01-01

    Travelling implies the encounter and, in certain cases, the clash of cultures. By interpreting translation as a means of transfer between languages and cultures, the present study looks into the different views on translating culture-specific realia. Through a close analysis of texts with the purpose of informing foreign tourists it offers ways of how to carry out this mediation. The study calls for further research into closely related issues, such as the translator’s cultural awareness of a...

  20. Pure and Modified Co-Poly(amide-12-b-ethylene oxide Membranes for Gas Separation Studied by Molecular Investigations

    Directory of Open Access Journals (Sweden)

    Luana De Lorenzo

    2012-06-01

    Full Text Available This paper deals with a theoretical investigation of gas transport properties in a pure and modified PEBAX block copolymer membrane with N-ethyl-o/p-toluene sulfonamide (KET as additive molecules. Molecular dynamics simulations using COMPASS force field, Gusev-Suter Transition State Theory (TST and Monte Carlo methods were used. Bulk models of PEBAX and PEBAX/KET in different copolymer/additive compositions were assembled and analyzed to evaluate gas permeability and morphology to characterize structure-performance relationships.

  1. Software theory a cultural and philosophical study

    CERN Document Server

    Frabetti, Federica

    2014-01-01

    This book engages directly in close readings of technical texts and computer code in order to show how software works. It offers an analysis of the cultural, political, and philosophical implications of software technologies that demonstrates the significance of software for the relationship between technology, philosophy, culture, and society.

  2. Convergence, Creative Industries and Civil Society Towards a New Agenda for Cultural Policy and Cultural Studies

    Directory of Open Access Journals (Sweden)

    Colin Mercer

    2009-10-01

    Full Text Available In this article I start with a personal experience "cameo" from 1996 in Australia and extrapolate from that some issues that remain relevant in the sometimes trou-bled relationship between cultural studies and cultural policy. These are encapsu-lated in the three "cs" of convergence, creative industries and civil society which provide a new context for both new research and new policy settings. The argu-ment is developed and situated in historical terms by examining the "cultural technologies", especially the newspaper, and subsequently print media in the 19th century, electronic media in the 20th century and digital media in the 21st century which provide the content, the technologies and the rituals for "imagining" our sense of place and belonging. This is then linked to ways of understanding culture and cultural technologies in the context of governmentality and the emergence of culture as a strategic object of policy with the aim of citizen- and population for-mation and management. This argument is then linked to four contemporary "testbeds" - cultural mapping and planning, cultural statistics and indicators, cul-tural citizenship and identity, and research of and for cultural policy - and priori-ties for cultural policy where cultural studies work has been extremely enabling and productive. The article concludes with an argument, derived from the early 20th century work of Patrick Geddes of the necessity of linking, researching, un-derstanding and operationalising the three key elements and disciplines of Folk (anthropology, Work (economics, and Place (geography in order to properly situate cultural policy, mapping and planning and their relationship to cultural studies and other disciplines.

  3. A comparative study of pure and copper (Cu)-doped ZnO nanorods for antibacterial and photocatalytic applications with their mechanism of action

    Science.gov (United States)

    Bhuyan, Tamanna; Khanuja, Manika; Sharma, R.; Patel, S.; Reddy, M. R.; Anand, S.; Varma, A.

    2015-07-01

    The present study reports the synthesis of pure and Cu-doped ZnO nanorods for antibacterial and photocatalytic applications. The samples were synthesized by simple, low cost mechanical-assisted thermal decomposition process. The synthesized materials were characterized by scanning electron microscopy, UV-Visible spectroscopy, and photoluminescence studies. The antibacterial activity of characterized samples was determined against Gram-positive bacteria such as Staphylococcus aureus and Streptococcus pyogenes and Gram-negative bacteria such as Escherichia coli using shake flask method with respect to time. The significant antibacterial activity was perceived from scanning electron micrographs that clearly revealed bacterial cell lysis resulting in the release of cytoplasmic content followed by cell death. The degradation of methylene blue was used as a model organic dye for photocatalytic activity. The present study demonstrates the superior photocatalytic and antibacterial activity of Cu-doped ZnO nanorods with respect to pure ZnO nanorods.

  4. A comparative study of pure and copper (Cu)-doped ZnO nanorods for antibacterial and photocatalytic applications with their mechanism of action

    Energy Technology Data Exchange (ETDEWEB)

    Bhuyan, Tamanna [Amity University, Uttar Pradesh, Amity Institute of Microbial Technology (India); Khanuja, Manika, E-mail: manikakhanuja@gmail.com; Sharma, R.; Patel, S.; Reddy, M. R.; Anand, S. [Amity University, Uttar Pradesh, Amity Institute of Nanotechnology (India); Varma, A. [Amity University, Uttar Pradesh, Amity Institute of Microbial Technology (India)

    2015-07-15

    The present study reports the synthesis of pure and Cu-doped ZnO nanorods for antibacterial and photocatalytic applications. The samples were synthesized by simple, low cost mechanical-assisted thermal decomposition process. The synthesized materials were characterized by scanning electron microscopy, UV–Visible spectroscopy, and photoluminescence studies. The antibacterial activity of characterized samples was determined against Gram-positive bacteria such as Staphylococcus aureus and Streptococcus pyogenes and Gram-negative bacteria such as Escherichia coli using shake flask method with respect to time. The significant antibacterial activity was perceived from scanning electron micrographs that clearly revealed bacterial cell lysis resulting in the release of cytoplasmic content followed by cell death. The degradation of methylene blue was used as a model organic dye for photocatalytic activity. The present study demonstrates the superior photocatalytic and antibacterial activity of Cu-doped ZnO nanorods with respect to pure ZnO nanorods.

  5. Density functional theory study of NO2-sensing mechanisms of pure and Ti-doped WO3 (002) surfaces

    Institute of Scientific and Technical Information of China (English)

    Hu Ming; Wang Wei-Dan; Zeng Jing; Qin Yu-Xiang

    2011-01-01

    Density functional theory (DFT) calculations are employed to explore the NO2-sensing mechanisms of pure and Ti-doped WO3 (002) surfaces.When Ti is doped into the WO3 surface,two substitution models are considered:substitution of Ti for W6c and substitution of Ti for Wsc.The results reveal that substitution of Ti for 5-fold W forms a stable doping structure,and doping induces some new electronic states in the band gap,which may lead to changes in the surface properties.Four top adsorption models of NO2 on pure and Ti-doped WO3 (002) surfaces are investigated:adsorptions on 5-fold W (Ti),on 6-fold W,on bridging oxygen,and on plane oxygen.The most stable and likely NO2 adsorption structures are both N-end oriented to the surface bridge oxygen O1c site.By comparing the adsorption energy and the electronic population,it is found that Ti doping can enhance the adsorption of NO2,which theoretically proves the experimental observation that Ti doping can greatly increase the WO3 gas sensor sensitivity to NO2 gas.

  6. Concepts of Culture in Middle Eastern and Islamic Studies

    Directory of Open Access Journals (Sweden)

    Pierre Hecker

    2017-01-01

    Full Text Available Introducing the seventh issue of META, this editorial discusses prevalent concepts of culture in Islamic and Middle Eastern Studies. Different conceptualizations of culture that explicitly or implicitly contain qualitative differentiations between cultures are revisited and discussed. Bearing considerable weight in the respective disciplines, the Islam-andthe-West paradigm, the delineation of diverging cultures along ethnic lines, the equation of culture with art or religion, and the culture-as-civilization paradigm are being scrutinized. Serving as an example for the confusion and lack of clarity regarding the concept of "culture", the book The Culture of Ambiguity by the German scholar of Islamic Studies Thomas Bauer is analyzed regarding its use of the term.

  7. Improving patient safety culture in general practice: An interview study

    NARCIS (Netherlands)

    N.J. Verbakel (Natasha J.); A.A. de Bont (Antoinette); T.J. Verheij; C. Wagner (Cordula); D.L.M. Zwart (Dorien Lyd Marieke)

    2015-01-01

    textabstractBackground When improving patient safety a positive safety culture is key. As little is known about improving patient safety culture in primary care, this study examined whether administering a culture questionnaire with or without a complementary workshop could be used as an interventio

  8. Do organizations reflect national cultures? A 10-nation study

    NARCIS (Netherlands)

    van Oudenhoven, JP

    2001-01-01

    The main purpose of this study was to cross-validate Hofstede's classification of national cultures. An additional aim was to investigate the relationship between culture as perceived and culture as desired. Over 800 advanced students of economics, business administration and management from 10 coun

  9. Japanese Culture: A Study of Origins and Characteristics.

    Science.gov (United States)

    Ishida, Eiichiro

    The culture and people of Japan are described from an ethnological/cultural anthropology viewpoint. The author begins his description with an introduction to the study of cultures and peoples. He then discusses the Japanese as a racial, ethnic, and language group. He later presents a historical/anthropological discussion of the formation of the…

  10. "Pure" cutaneous histiocytosis-X.

    Science.gov (United States)

    Wolfson, S L; Botero, F; Hurwitz, S; Pearson, H A

    1981-11-15

    The case histories of two young children who experienced skin rashes involving various areas of the body are reported. The diagnosis of pure cutaneous histiocytosis-X was established after extensive studies revealed no other organ involvement. The patients were treated with oral corticosteroids. Currently, both children are in good health, show no evidence of disease, and have been followed over a four-to-five-year period. Therapy with corticosteroids may not be indicated with pure cutaneous histiocytosis-X unless there is evidence of extracutaneous dissemination or rapid progression of the disease.

  11. Studies about an Equation of State for Pure Associated Fluids: Temperature Dependent Co-Volume Accounting a Physically Consistent Repulsive Term

    OpenAIRE

    Ricardo Figueiredo Checoni; Ravagnani, S. P.

    2012-01-01

    Studies related to the development of equations of state (EOS) to represent thermophysical properties of pure compounds are considered as important tools for engineers to design and optimize industrial equipment and processes. Furthermore, these tools also contribute to amplify the researchers’ knowledge related to molecular interaction types, in attempting to predict and correlate both energetic and volumetric effects existing in the compounds. From several equations of state existing, the c...

  12. Importance of Hofstede's study of cultural dimensions to the field of cross-cultural communication

    Institute of Scientific and Technical Information of China (English)

    周斌

    2008-01-01

    In 1980, Culture's Consequences: International Differences in Work-Related Values showed Geert Hofstede' s study of five cuhural dimensions: individualism versus collectivism, masculinity versus femininity, power distance, uncertainty avoidance and long-term versus short-term orientation to life. This essay discusses this theory' s weakness and usefulness as well as its importance to the cross-cultural communication that will give readers a better understanding about this cultural dimension. It concludes that Geert Hofstede's study of these five cultural dimensions is a starting point to the beginners to know about this field and the government offices even can establish economic policies according to the specific culture. It is really a useful research to the field of cross-cultural communication.

  13. Using activity theory to study cultural complexity in medical education.

    Science.gov (United States)

    Frambach, Janneke M; Driessen, Erik W; van der Vleuten, Cees P M

    2014-06-01

    There is a growing need for research on culture, cultural differences and cultural effects of globalization in medical education, but these are complex phenomena to investigate. Socio-cultural activity theory seems a useful framework to study cultural complexity, because it matches current views on culture as a dynamic process situated in a social context, and has been valued in diverse fields for yielding rich understandings of complex issues and key factors involved. This paper explains how activity theory can be used in (cross-)cultural medical education research. We discuss activity theory's theoretical background and principles, and we show how these can be applied to the cultural research practice by discussing the steps involved in a cross-cultural study that we conducted, from formulating research questions to drawing conclusions. We describe how the activity system, the unit of analysis in activity theory, can serve as an organizing principle to grasp cultural complexity. We end with reflections on the theoretical and practical use of activity theory for cultural research and note that it is not a shortcut to capture cultural complexity: it is a challenge for researchers to determine the boundaries of their study and to analyze and interpret the dynamics of the activity system.

  14. A Thorough Study between Language and Culture

    Institute of Scientific and Technical Information of China (English)

    于莹

    2016-01-01

    Nowadays, language skill has already become an essential skill for modern people in this increasingly competitive en-vironment. Some of those training institutions and schools may only focus on teaching the language without introduction of the culture behind it. So such language education is a long-term and profitable teaching process for the students? This essay will ex-plore the relationship between language and culture and gives advice on how to learn the language.

  15. A comparative study of protocols for mouse embryonic stem cell culturing.

    Directory of Open Access Journals (Sweden)

    Christoffer Tamm

    Full Text Available Most stem cell laboratories still rely on old culture methods to support the expansion and maintenance of mouse embryonic stem (ES cells. These involve growing cells on mouse embryonic fibroblast feeder cells or on gelatin in media supplemented with fetal bovine serum and leukemia inhibitory factor (LIF. However, these techniques have several drawbacks including the need for feeder-cells and/or use of undefined media containing animal derived components. Culture of stem cells under undefined conditions can induce spontaneous differentiation and reduce reproducibility of experiments. In recent years several new ES cell culture protocols, using more well-defined conditions, have been published and we have compared the standard culture protocols with two of the newly described ones: 1 growing cells in semi-adherence in a medium containing two small molecule inhibitors (CHIR99021, PD0325901 and; 2 growing cells in a spheroid suspension culture in a defined medium containing LIF and bFGF. Two feeder-dependent mouse ES (mES cell lines and two cell lines adapted to feeder-independent growth were used in the study. The overall aim has not only been to compare self-renewal and differentiation capacity, but also ease-of-use and cost efficiency. We show that mES cells when grown adherently proliferate much faster than when grown in suspension as free-floating spheres, independent of media used. Although all the tested culture protocols could maintain sustained pluripotency after prolonged culturing, our data confirm previous reports showing that the media containing two chemical inhibitors generate more pure stem cell cultures with negligible signs of spontaneous differentiation as compared to standard mES media. Furthermore, we show that this medium effectively rescues and cleans up cultures that have started to deteriorate, as well as allow for effective adaption of feeder-dependent mES cell lines to be maintained in feeder-free conditions.

  16. Dilemmas of Cultural Mediation: A Case Study of Tourism

    Directory of Open Access Journals (Sweden)

    Ajtony Zsuzsanna

    2015-12-01

    Full Text Available Travelling implies the encounter and, in certain cases, the clash of cultures. By interpreting translation as a means of transfer between languages and cultures, the present study looks into the different views on translating culture-specific realia. Through a close analysis of texts with the purpose of informing foreign tourists it offers ways of how to carry out this mediation. The study calls for further research into closely related issues, such as the translator’s cultural awareness of and sensitivity to similarity and difference, identity and alterity in culture.

  17. Multiplex PCR for specific and robust detection of Xanthomonas campestris pv. musacearum in pure culture and infected plant material

    DEFF Research Database (Denmark)

    Adriko, John; Aritua, V.; Mortensen, Carmen Nieves

    2012-01-01

    The present study developed a pathovar-specific PCR for the detection of Xanthomonas campestris pv. musacearum (Xcm), the cause of banana xanthomonas wilt, by amplification of a 265-bp region of the gene encoding the general secretion pathway protein D (GspD). A distinct DNA fragment of the expec......The present study developed a pathovar-specific PCR for the detection of Xanthomonas campestris pv. musacearum (Xcm), the cause of banana xanthomonas wilt, by amplification of a 265-bp region of the gene encoding the general secretion pathway protein D (GspD). A distinct DNA fragment...... was subsequently demonstrated in tests on artificially inoculated screenhouse cultivars of banana and field bananas with and without symptoms sampled from different parts of Uganda. This study therefore demonstrated a robust and specific Xcm diagnostic tool with the added advantage of applying internal PCR...

  18. Karl Marx and the Study of Media and Culture Today

    Directory of Open Access Journals (Sweden)

    Christian Fuchs

    2014-02-01

    Full Text Available The task of this paper discusses the role of Marx in analysing media, communica-tion and culture today. An analysis of three contemporary Cultural Studies works – Lawrence Grossberg’s monograph Cultural Studies in the Future Tense, John Hartley’s monograph Digital Futures for Cultural and Media Studies and Paul Smith’s edited volume The Renewal of Cultural Studies – shows that there is an agreement that the economy needs to be taken more into account by Cultural Studies, but disagreement on which approach should be taken and what the role of Karl Marx’s works shall be. The paper argues that Marx’s labour theory of value is especially important for critically analysing the media, culture and communica-tion. Labour is still a blind spot of the study of culture and the media, although this situation is slowly improving. It is maintained that the turn away from Marx in Cultural and Media Studies was a profound mistake that should be reverted. Only an engagement with Marx can make Cultural and Media Studies topical, politically relevant, practical and critical, in the current times of global crisis and resurgent critique.

  19. Contributions to the phytocoenological study of pure european beech forests in Oraştie river basin (central-western Romania

    Directory of Open Access Journals (Sweden)

    Petru BURESCU

    2012-05-01

    Full Text Available În the current paper we present a phytocoenologic study of the phytocoenoses of the association Festuco drymejaeFagetum Morariu et al. 1968 (Syn.: Fagetum sylvaticae transylvaticum facies with Festuca drymeja I. Pop et al. 1974, found in the pure European beech forests of the Orăştie river basin, lying in the central-western part of Romania. The characterisation of the association under analysis as well as the presentation of the synthetic table have been done byselecting the most representative relevées of pure European beech forests belonging to the Orăştie river basin. The phytocoenoses of these beech forests were analysed in terms of physiognomy and floristic composition, life forms spectrum, floristic elements, and ecological indices.

  20. Characteristic chromatographic fingerprint study of short-chain fatty acids in human milk, infant formula, pure milk and fermented milk by gas chromatography-mass spectrometry.

    Science.gov (United States)

    Jiang, Zhenzuo; Liu, Yanan; Zhu, Yan; Yang, Jing; Sun, Lili; Chai, Xin; Wang, Yuefei

    2016-09-01

    Human milk, infant formula, pure milk and fermented milk as food products or dietary supplements provide a range of nutrients required to both infants and adults. Recently, a growing body of evidence has revealed the beneficial roles of short-chain fatty acids (SCFAs), a subset of fatty acids produced from the fermentation of dietary fibers by gut microbiota. The objective of this study was to establish a chromatographic fingerprint technique to investigate SCFAs in human milk and dairy products by gas chromatography coupled with mass spectrometry. The multivariate method for principal component analysis assessed differences between milk types. Human milk, infant formula, pure milk and fermented milk were grouped independently, mainly because of differences in formic acid, acetic acid, propionic acid and hexanoic acid levels. This method will be important for the assessment of SCFAs in human milk and various dairy products.

  1. From Mental Game to Cultural Praxis: A Cultural Studies Model's Implications for the Future of Sport Psychology

    Science.gov (United States)

    Ryba, Tatiana V.; Wright, Handel Kashope

    2005-01-01

    This paper explores the implications of a cultural studies as praxis heuristic "model: for transforming sport psychology". It provides a brief introduction to both cultural studies and sport psychology and discusses a cultural studies intersection with sport studies and sport psychology. Cultural studies, it asserts, provides one of several…

  2. Comparative evaluation of the three different surface treatments - conventional, laser and Nano technology methods in enhancing the surface characteristics of commercially pure titanium discs and their effects on cell adhesion: An in vitro study.

    Science.gov (United States)

    Vignesh; Nayar, Sanjna; Bhuminathan; Mahadevan; Santhosh, S

    2015-04-01

    The surface area of the titanium dental implant materials can be increased by surface treatments without altering their shape and form, thereby increasing the biologic properties of the biomaterial. A good biomaterial helps in early cell adhesion and cell signaling. In this study, the commercially pure titanium surfaces were prepared to enable machined surfaces to form a control material and to be compared with sandblasted and acid-etched surfaces, laser treated surfaces and titanium dioxide (20 nm) Nano-particle coated surfaces. The surface elements were characterized. The biocompatibility was evaluated by cell culture in vitro using L929 fibroblasts. The results suggested that the titanium dioxide Nano-particle coated surfaces had good osteoconductivity and can be used as a potential method for coating the biomaterial.

  3. Host/patogen interactions between Scots pine seedlings (Pinus sylvestris L. and the p-strains of Heterobasidion annosum (Fr. Bref. in pure culture

    Directory of Open Access Journals (Sweden)

    Antoni Werner

    2014-01-01

    Full Text Available Pinus sylvestris L. seedlings representing half-sib families of several trees of known resistance in field conditions were inoculated with three strains of Heterobasidion annosum (Fr. Bref. representing the P intersterility group. Standardized inoculation procedure and uniform in vitro conditions allowed for evaluation of the genetically controlled host/pathogen interactions. Early and advanced stages of infection after prolonged exposure to the fungus were studied with the help of light and transmission electron microscopy. Abundance of osmiophilic material in vacuoles, necrotic reaction of cortical cells and secondary thickenings of cell walls in root regions in proximity to external mycelium were responsible for transient stagnation of pathogen spread in the cortex of resistant seedlings. Immobilized and degenerating hyphae inside vacuolar deposits in cortical cells of short roots of resistant plants are indicative of elevated toxicity of the protective materials at the initial stage of infection. Structural changes of vacuolar deposits and their subsequent disappearance, which coincided with heavy colonization of cortical cells of moderately susceptible seedlings, preceded hyphal penetration into the stele within 2-6-months after inoculation. Despite severe damage to root caps, there was no further spread of the fungus into meristems of growing roots and short dormant roots. , In susceptible seedlings, lack of a hypersensitive reaction and apparently low levels of osmiophilic material resulted in quick colonization of cortical cells, breakdown of endodermis and root abortion after entering the stele.

  4. Rereading of the Design of New College English at Culture Level and Culture Studies

    Institute of Scientific and Technical Information of China (English)

    葛维莉

    2012-01-01

    In order to know the whole procedures of a thesis and improve my own ability of writing a thesis, the thesis of The Design of New College English at Culture Level and Culture Studies which is a graduation thesis of master degree will be reread.

  5. Data handling in cross-cultural studies

    DEFF Research Database (Denmark)

    Scholderer, Joachim

    2010-01-01

    Sound marketing research can significantly reduce the risk associated with new product decisions. In a globalised economy, this often requires the collection of market and consumer data across different countries, cultures, and language communities. However, are such data actually comparable? Thi......, model specification, estimation, testing, and interpretation. The chapter includes a worked example, complete with command syntax for three different software packages.......? This chapter will familiarise the reader with a set of statistical techniques by which the cross-cultural comparability of data - their measurement invariance - can explicitly be assessed. The statistical framework (multi-group confirmatory factor analysis) is described in detail, including data requirements...

  6. The Comparison of Double J Stent Insertion and Conservative Treatment Alone in Severe Pure Gestational Hydronephrosis: A Case Controlled Clinical Study

    Directory of Open Access Journals (Sweden)

    Kürşat Çeçen

    2014-01-01

    Full Text Available Objective. Management options of gestational hydronephrosis are based on the coexisting stone disease, pyelonephritis, and renal disease. However, the management option and its consequences in the absence of a coexisting disease state are not clear. In this study we aimed to compare the effectiveness of conservative treatment and double J insertion in symptomatic pure gestational hydronephrosis. Material and Methods. The data of the women with severe pure gestational hydronephrosis over a nine-year period was collected retrospectively. The included women were grouped into two according to receiving double J stent insertion or conservative treatments. Results. Double J insertion and conservative treatment groups included 24 and 29 women, respectively. Hydronephrosis was demonstrated on the right, left, or both kidneys in 37 (70%, 13 (24%, and 3 (6% women, respectively. None of the participants gave birth prior to the 37th week. The demographics, initial pain scores, the severity of the hydronephrosis during first admission, and pain scores one week after the interventions did not differ significantly between groups (P>0.05. Similarly, the rates of complications, postpartum pain scores, and permanent hydronephrosis did not differ between groups (P>0.05. Conclusion. Double J insertion in symptomatic pure gestational hydronephrosis adds no benefit to conservative treatment.

  7. 纯种固态工艺酿造醋的风味研究%Flavor of vinegar with pure-culture solid-state fermentation

    Institute of Scientific and Technical Information of China (English)

    刘超; 鲁梅芳; 王春玲; 侯丽华

    2012-01-01

    通过对纯种固态酿醋工艺的有机酸、氨基酸和香气物质变化情况进行跟踪分析,系统全面地研究了纯种固态酿醋工艺的风味变化情况,为今后改善其风味奠定基础.食醋中的有机酸主要为乙酸、琥珀酸、苹果酸和乳酸.本工艺下食醋醋醅结束样中的氨基酸总量为50].52mg/L,总量比酒醅结束样增加了3倍左右,甜味氮基酸的含量从28.97mg/L增加到256.12mg/L.通过固相微萃取和气质联用技术对食醋酿造过程中香气成分进行分析发现酯类在第14d累积到最大值,之后略有下降;醇类在酒精发酵阶段大量生成,在醋酸发酵阶段由于与酸类酯化而大量减少;酸类在醋酸发酵阶段大量生成,之后由于酯化速度大于生酸速度而产生相对含量下降的趋势.%The flavor was studied systematically by the determination of organic acids, amino acids and aroma compounds in the solid-state fermentation vinegar, which laid the foundation of improving the flavor. The main organic acids were acetic acid, succinic acid, malic acid and lactic acid. When the fermentation was finished, the content of amino acid was 501.52mg/L, which was increased by 3 folds compared to the sample of ethanol fermentation. The content of sweet amino acid was increased from 28.97mg/L to 256.12mg/L. The aroma compounds were analyzed by SPME method and GC-MS, and the result indicated that esters accumulated to the maximum on 14th day and reduced later; alcohols greatly increased during the ethanol fermentation, and reduced to a low level because of esterification in the acetic acid fermentation; acids was mainly produced in the acetic acid fermentation and its relative content began to fall because esterification rate was greater than acidific rate.

  8. Tame Kernels of Pure Cubic Fields

    Institute of Scientific and Technical Information of China (English)

    Xiao Yun CHENG

    2012-01-01

    In this paper,we study the p-rank of the tame kernels of pure cubic fields.In particular,we prove that for a fixed positive integer m,there exist infinitely many pure cubic fields whose 3-rank of the tame kernel equal to m.As an application,we determine the 3-rank of their tame kernels for some special pure cubic fields.

  9. Pure Hydrogen Production in Membrane Reactor with Mixed Reforming Reaction by Utilizing Waste Gas: A Case Study

    Directory of Open Access Journals (Sweden)

    Seyyed Mohammad Jokar

    2016-09-01

    Full Text Available A rise in CO2 and other greenhouse gases’ concentration from gas refinery flares and furnaces in the atmosphere causes environmental problems. In this work, a new process was designed to use waste gas (flue gas and flare gas of a domestic gas refinery to produce pure hydrogen in a membrane reactor. In particular, the process foresees that the energy and CO2 content of flue gas can provide the heat of the mixed reforming reaction to convert flare gas into hydrogen. Furthermore, the characteristics of the feed stream were obtained via simulation. Then, an experimental setup was built up to investigate the performance of a membrane reactor allocating an unsupported dense Pd-Ag membrane at the mentioned conditions. In this regard, a Ni/CeO2 catalyst was loaded in the membrane reformer for mixed reforming reaction, operating at 450 °C, in a pressure range between 100 and 350 kPa and a gas hourly space velocity of around 1000 h−1. The experimental results in terms of methane conversion, hydrogen recovery and yield, as well as products’ compositions are reported. The best results of this work were observed at 350 kPa, where the MR was able to achieve about 64%, 52% and 50% for methane conversion, hydrogen yield and recovery, respectively. Furthermore, with the assistance of the experimental tests, the proposed process was simulated in the scaling up to calculate the needed surface area for MR in the domestic gas refinery.

  10. A CRIS in the Desert: The Implementation of Pure at KAUST: A Case Study in Information Exchange

    KAUST Repository

    Grenz, Daryl M.

    2016-06-10

    The integration of research information systems with existing university processes has tended towards information exchange models in which the CRIS ingests information from existing systems and takes on functions that were previously distributed across several independent solutions. This paper draws upon the experience of the implementation of a CRIS at the King Abdullah University of Science and Technology (KAUST) to posit a model in which functions remain distributed so as to take advantage of the strengths of each system. The functions discussed include institutional reporting, publications tracking, preservation of research outputs, provision of public access, researcher identity and profiling, and metrics analysis. The systems reviewed include a CRIS (Pure), a locally developed publications tracking system, a hosted DSpace repository, a locally developed ORCID integration, and a metrics dashboard (PlumX). The interactions between these systems forms a network of services to our research community, with each node connected to several others, and we discuss how we arrived at the current arrangement, as well as its drawbacks and advantages. The still limited use of standard data exchange formats like CERIF XML is discussed as a constraint that increases the costs of adding to and maintaining the network of services. At the same time we look at how increased standardization should make this distributed approach sustainable, allowing institutions like ours to mix and match complementary systems to achieve an optimal set of research information services for our needs.

  11. Ab initio LSDA and LSDA+U study of pure and Cd-doped cubic lanthanide sesquioxides

    DEFF Research Database (Denmark)

    Richard, D.; Muñoz, E.L.; Rentería, M.

    2013-01-01

    approximation (LSDA) and the Coulomb-corrected LSDA+U. In the case of the pure systems, our calculations show that LSDA+U gives a better representation of the band structure compared to LSDA. The predicted equilibrium structures and the electric field gradient (EFG) tensor at Ln sites were calculated...... and compared with those obtained by means of hyperfine techniques and with theoretical results obtained in In2O3, Sc2O3, and Lu2O3 reported in the literature. The origin of the EFG at Ln sites and the role played by the 4f electrons on this quantity are discussed. In the case of the Cd-doped systems, the APW......+lo method (also within LSDA and LSDA+U) was applied to treat the electronic structure of the doped system. The role of the Ln 4felectrons on the EFG at Cd impurity sites, and other variables like structural distortions induced by the Cd impurity, were investigated in detail and are discussed and compared...

  12. In-depth Cultural Studies in Multicultural Group

    Directory of Open Access Journals (Sweden)

    Siliņa-Jasjukeviča Gunta

    2016-12-01

    Full Text Available There is much research and educational practices at all levels of education on how to deal with promoting acceptance and understanding between different cultures. A cultural study forms an important part of shaping intercultural understanding. The aim of the research is to analyze an innovative way of incorporating cultural studies in teacher education program from the perspective of encouraging multinational students to reveal common values within diverse manifestations of different cultures. The present article describes a qualitative study of multinational students’ experiences in international project related to the learning about Nordic and Baltic cultural traditions. In the conclusion of the article, the efficiency of the structure of content and the process of in-depth cultural studies are analyzed. The discussion contains problems for further research of this topic.

  13. Qualitative study of three cell culture methods.

    Science.gov (United States)

    Wang, Aiguo; Xia, Tao; Ran, Peng; Chen, Xuemin; Nuessler, Andreas K

    2002-01-01

    Primary rat hepatocytes were cultured using different in vitro models and the enzyme leakage, albumin secretion, and cytochrome P450 1A (CYP 1A) activity were observed. The results showed that the level of LDH was decreased over time in culture. However, on day 5, LDH showed a significant increase in monolayer culture (MC) while after day 8 no LDH was detectable in sandwich culture (SC). The levels of AST and ALT did not change significantly over the investigated time. The CYP 1A activity was gradually decreased in a time-dependent manner in MC and SC. The decline of CYP 1A was faster in MC than in SC. This effect was partially reversed by using cytochrome P450 (CYP450) inducer such as Omeprazol and 3-methylcholanthrene (3-MC) and the CYP 1A induction was always higher in MC than in SC. In bioreactor basic CYP 1A activity was preserved over 2 weeks and the highest albumin production was observed in bioreactor followed by SC and MC. Taken together, it was indicated each investigated model had its advantages and disadvantages. It was also underlined that various in vitro models may address different questions.

  14. Amnesiology: Towards the study of cultural oblivion

    NARCIS (Netherlands)

    Plate, L.

    2016-01-01

    It is generally accepted that memory is a dialectic involving both remembering and forgetting. Also, there is agreement among cultural memory scholars that acts of memory seek to counter the effects of forgetting: they serve the imperative to remember and impede the work of forgetting. This article

  15. Geographical Study of American Blues Culture

    Science.gov (United States)

    Strait, John B.

    2010-01-01

    Music is not often utilized in teaching geography, despite the fact that many scholars orient their research around analyzing both the historical and spatial dimensions of musical expression. This article reports on the use of a teaching module that utilizes blues culture as a lens to understand the geographical history of the United States. The…

  16. Data handling in cross-cultural studies

    DEFF Research Database (Denmark)

    Scholderer, Joachim

    2010-01-01

    Sound marketing research can significantly reduce the risk associated with new product decisions. In a globalised economy, this often requires the collection of market and consumer data across different countries, cultures, and language communities. However, are such data actually comparable? Thi...

  17. Why Lesson Study Works in Japan: A Cultural Perspective

    Science.gov (United States)

    Ebaeguin, Marlon; Stephens, Max

    2014-01-01

    Japanese lesson study has attracted many international educators who have been impressed by its capacity to foster student learning and sustained professional growth of teachers. This paper reports a study on its cultural orientations that may explain why lesson study works seamlessly in Japan. Hofstede's dimensions of national culture are…

  18. Functional genomic study of the environmentally important Desulfovibrio /Methanococcus syntrophic co-culture.

    Science.gov (United States)

    Mukhopadhyay, A.

    2008-12-01

    The use of microbe-oriented bioremediation for ameliorating extensive environmental pollution has fostered fundamental and applied studies of environmentally relevant microorganisms such as Desulfovibrio vulgaris, Shewanella oneidensis and Geobacter metallireducens.. Concurrently, there has been an increasing appreciation that the physiology of these organisms in pure culture is not necessarily representative of its activities in the environment. To enable a better understanding of microbial physiology under more environmentally relevant conditions, the syntrophic growth between the sulfate reducing bacterium, D. vulgaris and the hydrogenotrophic methanogen, Methanococcus maripaludis serves as an ideal system for laboratory studies. Cell wide analyses using transcript, proteomics and metabolite analysis have been widely used to understand cellular activity at a molecular level. Using D. vulgaris and M. maripaludis arrays, and the iTRAQ proteomics method, we studied the physiology of the D. vulgaris / M. maripaludis syntrophic co- cultures. The results from this study allowed us to identify differences in cellular response in mono-culture vs. co-culture growth for both D. vulgaris and M. maripaludis.

  19. STUDY OF CULTURING CARDIOVASCULAR TISSUE IN VITRO

    Institute of Scientific and Technical Information of China (English)

    成少飞; 叶清

    2004-01-01

    Objective To evaluate the feasibility of utilizing vascular cells combined with folded and framed culture model to develop completely autologous human tissue without using any scaffold material under the principles of Tissue Engineering. Methods Human vascular cells cultured from ascending aorta (group A) and saphenous vein (group B) were seeded into 15cm-dishes (each n =12 ) and cultured to form cell sheets over a period of four weeks with Dulbecco's modified Eagle's medium supplemented with lmmol/L L-ascorbic acid 2-phosphate. Thereafter, cell sheets (6 samples of each group) were four-layer folded and cultured in a newly developed frame device for additional four weeks. Controls remained under standard culture conditions. Tissue development was evaluated by light and electron microscopy, biochemical assays. Results The formation of multi-layered cell sheets and production of extracellular matrix were observed in each group after the initial four weeks. Analysis of the folded and framed neo-tissue revealed a solid structure with increased matrix formation and tissue organization compared to the control groups after additional four weeks. DNA assay indicated significantly lower cell proliferation in folded and framed cell sheets than in that of unframed counterparts. Yet hydroxyproline assay demonstrated significant increase of collagen content in the framed aortic and venous derived tissues, which contained 82 % and 42 % that of human pericardium. Conclusion It is feasible to obtain completely autologous human cardiovascular tissue with the alternative new approach. Numerous issues including improvement of mechanical strength of neo-tissue remain to be investingated.

  20. Preliminary Study of Realistic Blast Impact on Cultured Brain Slices

    Science.gov (United States)

    2015-04-01

    hippocampal slice samples to better understand blast-induced brain damage. 15. SUBJECT TERMS RDX spheres , organotypic cultures of hippocampus, small...Preliminary Study of Realistic Blast Impact on Cultured Brain Slices by Thuvan Piehler, Rohan Banton, Lars Piehler, Richard Benjamin, Ray...Aberdeen Proving Ground, MD 21005-5066 ARL-TR-7197 April 2015 Preliminary Study of Realistic Blast Impact on Cultured Brain Slices Thuvan

  1. From Cultural Studies to Cultural Research: Engaged Scholarship in the Twenty-first Century

    Directory of Open Access Journals (Sweden)

    Ien Ang

    2011-11-01

    Full Text Available Is, or should cultural studies be, a discipline or not? What exactly is its object? Should cultural studies be focused on influencing policy or be an agent of critique? What is the role of theory? What kind of theory? Should textual analysis or ethnography predominate? The regular reiteration of such questions reveals an ongoing sense of crisis, a general apprehensiveness over the question whether cultural studies is able to live up to its own self-declared aspirations, both intellectually and politically.

  2. From Cultural Studies to Cultural Research: Engaged Scholarship in the Twenty-first Century

    Directory of Open Access Journals (Sweden)

    Ien Ang

    2011-11-01

    Full Text Available  Is, or should cultural studies be, a discipline or not? What exactly is its object? Should cultural studies be focused on influencing policy or be an agent of critique? What is the role of theory? What kind of theory? Should textual analysis or ethnography predominate? The regular reiteration of such questions reveals an ongoing sense of crisis, a general apprehensiveness over the question whether cultural studies is able to live up to its own self-declared aspirations, both intellectually and politically.

  3. Dahlbeck and Pure Ontology

    Science.gov (United States)

    Mackenzie, Jim

    2016-01-01

    This article responds to Johan Dahlbeck's "Towards a pure ontology: Children's bodies and morality" ["Educational Philosophy and Theory," vol. 46 (1), 2014, pp. 8-23 (EJ1026561)]. His arguments from Nietzsche and Spinoza do not carry the weight he supposes, and the conclusions he draws from them about pedagogy would be…

  4. Dahlbeck and Pure Ontology

    Science.gov (United States)

    Mackenzie, Jim

    2016-01-01

    This article responds to Johan Dahlbeck's "Towards a pure ontology: Children's bodies and morality" ["Educational Philosophy and Theory," vol. 46 (1), 2014, pp. 8-23 (EJ1026561)]. His arguments from Nietzsche and Spinoza do not carry the weight he supposes, and the conclusions he draws from them about pedagogy would be…

  5. Artefacts in 1H NMR-based metabolomic studies on cell cultures.

    Science.gov (United States)

    Madhu, Basetti; Dadulescu, Madalina; Griffiths, John

    2015-04-01

    Metabolomic studies on cultured cells involve assays of cell extracts and culture medium, both of which are often performed by (1)H NMR. Cell culture is nowadays performed in plastic dishes or flasks, and the extraction of metabolites from the cells is typically performed with perchloric acid, methanol-chloroform, or acetonitrile, ideally while the cells are still adherent to the culture dish. We conducted this investigation to identify contaminants from cell culture plasticware in metabolomic studies. Human diploid fibroblasts (IMR90) (n = 6), HeLa cells (n = 6), and transformed astrocytes with HIF-1 knockout (Astro-KO) (n = 6) were cultured. Cells were seeded in 100 mm Petri dishes with 10 ml complete growth medium (Dulbecco's minimum essential medium) containing 10 % foetal bovine serum (FBS). Cell cultures were incubated at 37 °C in 5 % CO2 for approximately 3 days. Metabolites were extracted by use of a perchloric acid procedure. (1)H NMR spectroscopy was used for metabolite analysis. "Null sample" (i.e. cell-free) experiments were performed by either rinsing dishes with medium or incubating the medium in Petri dishes from five different manufacturers for 72 h and then by performing a dummy "extraction" of each Petri dish by the perchloric acid, methanol-chloroform, or acetonitrile procedures. Principal components analysis was used for classification of samples and to determine the contaminants arising from plasticware. We found that even brief rinsing of cell culture plasticware with culture medium elutes artefactual chemicals, the (1)H NMR signals of which could confound assays of acetate, succinate, and glycolate. Incubation of culture medium in cell-culture dishes for 72 h (as in a typical cell-culture experiment) followed by perchloric extraction in the dishes enhanced elution of the artefacts. These artefacts were present, but somewhat less pronounced, in the (1)H NMR spectra of null samples extracted with methanol and acetonitrile. Ethanol, lactate

  6. Studies on hydrogen generation characteristics of hydrolysis of the ball milling Al-based materials in pure water

    Energy Technology Data Exchange (ETDEWEB)

    Fan, Mei-Qiang [Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian (China). Materials and Thermochemistry Laboratory; Graduate School of the Chinese Academy of Sciences, Beijing (China); Xu, Fen; Sun, Li-Xian [Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian (China). Materials and Thermochemistry Laboratory

    2007-09-15

    In this paper, a series of Al-based materials were prepared by ball milling and/or melting. The XRD, SEM and TG-DTA techniques were used for sample analyses. Effects of different metals such as Zn, Ca, Ga, Bi, Mg, In and Sn on the hydrogen generation through hydrolysis of the Al alloy were evaluated in pure water. The results showed that mechanical milling was more favorable than the melting method to synthesize the Al alloys containing some metals with lower melting point and easier vaporization in the melting process. Addition of Bi and Sn could more significantly enhance Al reactivity with water in Al alloy than other metals such as Zn, Ca and Ga. Especially Al-Bi alloy had a faster hydrolysis rate than Al-Sn alloy at room temperature. For Al-Bi alloy, the addition of Zn and Ga accelerated the alloy hydrolysis while the effect of addition of other metals (Sn, In, Mg) on the hydrolysis of the alloy was reverse. Furthermore, the effect of some compounds (NaCl, MgCl{sub 2}, CaH{sub 2}) on the hydrolysis of the Al-Bi alloy was explored. It showed that the milling Al-Bi alloy together with the compounds could accelerate the formation of its mico-galvanic cell between the anode (Al) and cathode (Bi). The alloy composition was therefore optimized to be Bi, Zn, Ga, CaH{sub 2} and Al. The optimized Al alloy demonstrated a high hydrogen generation rate and theoretic hydrogen yields. (author)

  7. INGESTIVE BEHAVIOR OF GOATS IN RYEGRASS AND BLACK OAT PASTURES IN PURE OR MIXTURE CULTURE COMPORTAMENTO INGESTIVO DE CAPRINOS EM PASTAGEM DE AZEVÉM E AVEIA PRETA EM CULTIVO PURO E CONSORCIADO

    Directory of Open Access Journals (Sweden)

    Alda Lúcia Gomes Monteiro

    2009-07-01

    Full Text Available The experiment was realized in Campo Largo, PR, where the ingestive behavior of goats was evaluated under ryegrass (Lolium multiflorum Lam. and black oat (Avena strigosa Schreb pastures in pure or mixture culture, in the period of 04/07/2004 to 05/08/2004. The grasses were applied in poles of 630 m² each, and the experimental design was placed in randomized blocks with three treatments and three repetitions. Twelve female goats were distributed in three experimental poles with four goats each for grazing evaluations. Previously to the evaluations of the animals the measurements of the pasture were obtained, which included height, total mass of forage and of the compounds leaf and steam. The goats were evaluated by preference and ingestion rate. The averages of pastures height was higher (p>0.05 in ryegrass and mixture, and in other pastures evaluations ryegrass was superior (p<0.05 to the others treatments. The grazing time of goats in ryegrass and black oat was superior (p<0.05 to the mixture. The bite rate per minute was higher (p<0.05 in black oat. The goats demonstrated preference for ryegrass and black oat in pure culture.
     
    KEY WORDS: Avena strigosa Schreb, bite, goats, Lolium multiflorum Lam, preference. The experiment was realized in Campo Largo – PR, where the ingestive behavior of goats was evaluated under ryegrass (Lolium multiflorum Lam. and black oat (Avena strigosa Schreb pastures in pure or mixture culture, in the period of 04/07/2004 to 05/08/2004. The grasses were applied in poles of 630 m² each, and the experimental design was placed in randomized blocks with three treatments and three repetitions. Twelve female goats were distributed in three experimental poles with four goats each for grazing evaluations. Previously to the evaluations of the animals the measurements of the pasture were obtained, which included height, total mass of forage and of the compounds leaf and steam. The goats were evaluated by

  8. CULTURAL STUDIES: INSTITUTIONAL CRISIS OR THE TIME OF STRATEGIC DECISIONS

    Directory of Open Access Journals (Sweden)

    Irina Ya. Murzina

    2015-01-01

    Full Text Available The aim of this investigation is to signify of the problems of cultural studies as a science and educational practice in a situation of modernization of education. The publication continues the discussion started at the alignment meeting of Heads of Cultural Studies Departments in Russia – «The science of culture and cultural education: time for strategic decisions». Methods. The author has applied the following methods: analysis of existing points of view; reflection of the essence of the cultural knowledge and perspectives of cultural development of cultural studies science in Russia. Results. The research outcomes show that cultural studies in modern Russia are experiencing institutional crisis: insufficient definiteness and awareness by experts and publicity due to places and roles of culturological disciplines in educational process, but also the methodological bases of activity of culturologists. Aspects of culturological formation are considered and prospects of its development are specified. It was proposed to continue the discussion started at the alignment meeting of Herzen State Pedagogical University of Russia, and it will give an option to discuss the solution ways of current recessionary situation more fundamentally and subjectively on territory-regional level. Scientific novelty. The approaches to understanding the culture as areas of scientific knowledge are methodized; the areas of cultural education in the conditions of modernization are summarized. Practical significance. The research implementations can be used while improving the theoretical-methodological component of cultural education, and extending the horizons for topical current research works in the field of cultural studies

  9. A Cultural Interpretation of a Social Studies Curriculum.

    Science.gov (United States)

    Chilcott, John H.

    Social studies documents were collected from teachers in the Tucson, Arizona area and examined using three theories of culture as a way to explore the interrelationships between social studies curriculum and United States society. Malinowski's functionalist position suggests that culture is composed of traits each of which provide a specific…

  10. Third Culture Kids and College Support: A Case Study

    Science.gov (United States)

    Holdren, Sarah Jennifer

    2013-01-01

    This single site case study applies the "Transition Cycle" framework (Pollock & Van Reken, 2009) to an institutionally-based, student-run support program for Third Culture Kids. The purpose of this study was to examine how Lewis and Clark College responded to the presence of Third Culture Kid, or Global Nomad, students on campus by…

  11. Communicating Investment: Cultural Studies, Affect and the Academy

    Science.gov (United States)

    Gregg, Melissa

    2008-01-01

    Over the past decade, publishers' catalogues have showcased a continuing supply of introductory readers, taxonomies and evaluations of cultural studies, largely for teaching purposes. In this article, the author suggests that the current climate of academic publishing has allowed cultural studies' particular investment and commitment to scholarly…

  12. Cultural Awareness of Chinese English Teacher:a Case Study

    Institute of Scientific and Technical Information of China (English)

    ZHOU Hong; LUO Liu-ping

    2014-01-01

    As English is becoming an international Language, English teaching in China, especially at tertiary level, is transform-ing from linguistic study to interpersonal communication and cross-cultural communication. The case study of this article reaches a conclusion that English teachers should make every effort to cultivate cultural awareness.

  13. A case study of organisational Cultural Competence in mental healthcare.

    Science.gov (United States)

    Adamson, Jean; Warfa, Nasir; Bhui, Kamaldeep

    2011-09-15

    Ensuring Cultural Competence (CC) in health care is a mechanism to deliver culturally appropriate care and optimise recovery. In policies that promote cultural competence, the training of mental health practitioners is a key component of a culturally competent organisation. This study examines staff perceptions of CC and the integration of CC principles in a mental healthcare organisation. The purpose is to show interactions between organisational and individual processes that help or hinder recovery orientated services. We carried out a case study of a large mental health provider using a cultural competence needs analysis. We used structured and semi-structured questionnaires to explore the perceptions of healthcare professionals located in one of the most ethnically and culturally diverse areas of England, its capital city London. There was some evidence that clinical staff were engaged in culturally competent activities. We found a growing awareness of cultural competence amongst staff in general, and many had attended training. However, strategic plans and procedures that promote cultural competence tended to not be well communicated to all frontline staff; whilst there was little understanding at corporate level of culturally competent clinical practices. The provider organisation had commenced a targeted recruitment campaign to recruit staff from under-represented ethnic groups and it developed collaborative working patterns with service users. There is evidence to show tentative steps towards building cultural competence in the organisation. However, further work is needed to embed cultural competence principles and practices at all levels of the organisation, for example, by introducing monitoring systems that enable organisations to benchmark their performance as a culturally capable organisation.

  14. A case study of organisational cultural competence in mental healthcare

    Directory of Open Access Journals (Sweden)

    Bhui Kamaldeep

    2011-09-01

    Full Text Available Abstract Background Ensuring Cultural Competence (CC in health care is a mechanism to deliver culturally appropriate care and optimise recovery. In policies that promote cultural competence, the training of mental health practitioners is a key component of a culturally competent organisation. This study examines staff perceptions of CC and the integration of CC principles in a mental healthcare organisation. The purpose is to show interactions between organisational and individual processes that help or hinder recovery orientated services. Methods We carried out a case study of a large mental health provider using a cultural competence needs analysis. We used structured and semi-structured questionnaires to explore the perceptions of healthcare professionals located in one of the most ethnically and culturally diverse areas of England, its capital city London. Results There was some evidence that clinical staff were engaged in culturally competent activities. We found a growing awareness of cultural competence amongst staff in general, and many had attended training. However, strategic plans and procedures that promote cultural competence tended to not be well communicated to all frontline staff; whilst there was little understanding at corporate level of culturally competent clinical practices. The provider organisation had commenced a targeted recruitment campaign to recruit staff from under-represented ethnic groups and it developed collaborative working patterns with service users. Conclusion There is evidence to show tentative steps towards building cultural competence in the organisation. However, further work is needed to embed cultural competence principles and practices at all levels of the organisation, for example, by introducing monitoring systems that enable organisations to benchmark their performance as a culturally capable organisation.

  15. Study Progress on Tissue Culture of Maize Mature Embryo

    Science.gov (United States)

    Wang, Hongzhen; Cheng, Jun; Cheng, Yanping; Zhou, Xioafu

    It has been paid more and more attention on maize tissue culture as it is a basic work in maize genetic transformation, especially huge breakthrough has been made in maize tissue culture utilizing mature embryos as explants in the recent years. This paper reviewed the study progress on maize tissue culture and plant regeneration utilizing mature embryos as explants from callus induction, subculture, plant regeneration and browning reduction and so on.

  16. Isolation of intact and pure chloroplasts from leaves of Arabidopsis thaliana plants acclimated to low irradiance for studies on Rubisco regulation

    Directory of Open Access Journals (Sweden)

    Magda Grabsztunowicz

    2012-11-01

    Full Text Available A protocol is presented for low-cost and fast isolation of intact and pure chloroplasts from leaves of plants acclimated to low irradiance. The protocol is based on a differential centrifugation of cleared leaf homogenate and omits a centrifugation on Percoll gradient step. The intactness and purity of the chloroplasts isolated from leaves of low irradiance-acclimated plants by using this protocol (confirmed by phase contrast microscopy as well as enzymatic and immunological approaches allows plausible studies on low irradiance-dependent Rubisco regulation.

  17. Examining Culture's Impact on the Learning Behaviors of International Students from Confucius Culture Studying in Western Online Learning Context

    Science.gov (United States)

    Kang, Haijun; Chang, Bo

    2016-01-01

    There is a lack of shared understanding of how culture impacts learning in online environment. Utilizing document analysis, the authors in this research study culture's impact on the learning behaviors of student sojourners from Confucius culture studying in Western online learning context. The shared understandings of Confucius culture and…

  18. Effects of Improving Primary Health Care Workers’ Knowledge About Public Health Services in Rural China: A Comparative Study of Blended Learning and Pure E-Learning

    Science.gov (United States)

    Zhan, Xingxin; Zhang, Zhixia; Sun, Fang; Liu, Qian; Peng, Weijun; Zhang, Heng

    2017-01-01

    Background Primary health care workers (PHCWs) are a major force in delivering basic public health services (BPHS) in rural China. It is necessary to take effective training approaches to improve PHCWs’ competency on BPHS. Both electronic learning (e-learning) and blended learning have been widely used in the health workers’ education. However, there is limited evidence on the effects of blended learning in comparison with pure e-learning. Objective The aim of this study was to evaluate the effects of a blended-learning approach for rural PHCWs in improving their knowledge about BPHS as well as training satisfaction in comparison with a pure e-learning approach. Methods The study was conducted among PHCWs in 6 rural counties of Hubei Province, China, between August 2013 and April 2014. Three counties were randomly allocated blended-learning courses (29 township centers or 612 PHCWs—the experimental group), and three counties were allocated pure e-learning courses (31 township centers or 625 PHCWs—the control group). Three course modules were administered for 5 weeks, with assessments at baseline and postcourse. Primary outcomes were score changes in courses’ knowledge. Secondary outcome was participant satisfaction (5-point Likert scale anchored between 1 [strongly agree] and 5 [strongly disagree]). Results The experimental group had higher mean scores than the control group in knowledge achievement in three course modules: (1) module 1: 93.21 (95% CI 92.49-93.93) in experimental group versus 88.29 (95% CI 87.19-89.40) in the control group; adjusted difference, 4.92 (95% CI 2.61-7.24; Plearning group gave more positive responses with the four issues than control group participants: (1) the increase of interest in learning, 1.85 (95% CI 1.22-2.80; P=.003); (2) the increase of interaction with others, 1.77 (95% CI 1.20-2.60; P=.004); (3) the satisfaction with learning experience, 1.78 (95% CI 1.11-2.88; P=.02); and (4) achievement of learning objectives, 1

  19. Effects of Improving Primary Health Care Workers' Knowledge About Public Health Services in Rural China: A Comparative Study of Blended Learning and Pure E-Learning.

    Science.gov (United States)

    Zhan, Xingxin; Zhang, Zhixia; Sun, Fang; Liu, Qian; Peng, Weijun; Zhang, Heng; Yan, Weirong

    2017-05-01

    Primary health care workers (PHCWs) are a major force in delivering basic public health services (BPHS) in rural China. It is necessary to take effective training approaches to improve PHCWs' competency on BPHS. Both electronic learning (e-learning) and blended learning have been widely used in the health workers' education. However, there is limited evidence on the effects of blended learning in comparison with pure e-learning. The aim of this study was to evaluate the effects of a blended-learning approach for rural PHCWs in improving their knowledge about BPHS as well as training satisfaction in comparison with a pure e-learning approach. The study was conducted among PHCWs in 6 rural counties of Hubei Province, China, between August 2013 and April 2014. Three counties were randomly allocated blended-learning courses (29 township centers or 612 PHCWs-the experimental group), and three counties were allocated pure e-learning courses (31 township centers or 625 PHCWs-the control group). Three course modules were administered for 5 weeks, with assessments at baseline and postcourse. Primary outcomes were score changes in courses' knowledge. Secondary outcome was participant satisfaction (5-point Likert scale anchored between 1 [strongly agree] and 5 [strongly disagree]). The experimental group had higher mean scores than the control group in knowledge achievement in three course modules: (1) module 1: 93.21 (95% CI 92.49-93.93) in experimental group versus 88.29 (95% CI 87.19-89.40) in the control group; adjusted difference, 4.92 (95% CI 2.61-7.24; Plearning group gave more positive responses with the four issues than control group participants: (1) the increase of interest in learning, 1.85 (95% CI 1.22-2.80; P=.003); (2) the increase of interaction with others, 1.77 (95% CI 1.20-2.60; P=.004); (3) the satisfaction with learning experience, 1.78 (95% CI 1.11-2.88; P=.02); and (4) achievement of learning objectives, 1.63 (95% CI 1.08-2.48; P=.02). Among PHCWs in

  20. Purely Functional Structured Programming

    OpenAIRE

    Obua, Steven

    2010-01-01

    The idea of functional programming has played a big role in shaping today's landscape of mainstream programming languages. Another concept that dominates the current programming style is Dijkstra's structured programming. Both concepts have been successfully married, for example in the programming language Scala. This paper proposes how the same can be achieved for structured programming and PURELY functional programming via the notion of LINEAR SCOPE. One advantage of this proposal is that m...

  1. Purely Cortical Anaplastic Ependymoma

    Directory of Open Access Journals (Sweden)

    Flávio Ramalho Romero

    2012-01-01

    Full Text Available Ependymomas are glial tumors derived from ependymal cells lining the ventricles and the central canal of the spinal cord. It may occur outside the ventricular structures, representing the extraventicular form, or without any relationship of ventricular system, called ectopic ependymona. Less than fifteen cases of ectopic ependymomas were reported and less than five were anaplastic. We report a rare case of pure cortical ectopic anaplastic ependymoma.

  2. Purely tetrahedral quadruple systems

    Institute of Scientific and Technical Information of China (English)

    JI Lijun

    2006-01-01

    An oriented tetrahedron is a set of four vertices and four cyclic triples with the property that any ordered pair of vertices is contained in exactly one of the cyclic triples. A tetrahedral quadruple system of order n (briefly TQS(n)) is a pair (X,B), where X is an nelement set and B is a set of oriented tetrahedra such that every cyclic triple on X is contained in a unique member of B. A TQS(n) (X, B) is pure if there do not exist two oriented tetrahedra with the same vertex set. In this paper, we show that there is a pure TQS(n) if and only if n≡2,4(mod 6),n>4,or n≡1,5(mod 12). One corollary is that there is a simple two-fold quadruple system of order n if and only if n≡2,4 (mod 6) and n>4, or n≡1, 5 (mod 12).Another corollary is that there is an overlarge set of pure Mendelsohn triple systems of order n for n≡1,3(mod 6),n>3, or n≡0,4 (mod 12).

  3. Studies on experimental culture of a marine ciliate Fabrea salina

    Digital Repository Service at National Institute of Oceanography (India)

    Rattan, R.; Ansari, Z.A.; Chatterji, A.

    Studies were conducted on the culture of a marine ciliate, Fabrea salina in the laboratory condition. Three types of inert feed; commercial yeast, fermented wheat bran and fermented rise bran were tested to study their suitability as artificial feed...

  4. "Two Cultures" Topics for General Studies Science Courses.

    Science.gov (United States)

    Larson, James H.

    1982-01-01

    Theses proposed in C. P. Snow's book "The Two Cultures," including uncommunicative scientific and literary groups, gap between rich and poor, overpopulation, and nuclear war remain viable topics. Discusses the scientific and literary cultural gap and what can be done in general studies science courses to ameliorate the condition. (Author/JN)

  5. A Longitudinal Study on Newcomers' Perception of Organisational Culture

    Science.gov (United States)

    Turker, Duygu; Altuntas, Ceren

    2015-01-01

    Purpose: The purpose of this paper is to analyse how newcomers' perceptions of organisational culture can change over time. The study tries to address whether initial working experience changes newcomers' perceptions about the ideal organisational culture, and whether these perceptions converge with those of their supervisors.…

  6. A Longitudinal Study on Newcomers' Perception of Organisational Culture

    Science.gov (United States)

    Turker, Duygu; Altuntas, Ceren

    2015-01-01

    Purpose: The purpose of this paper is to analyse how newcomers' perceptions of organisational culture can change over time. The study tries to address whether initial working experience changes newcomers' perceptions about the ideal organisational culture, and whether these perceptions converge with those of their supervisors.…

  7. Rapid Rural Appraisal: A Study of Gullah Culture.

    Science.gov (United States)

    Jarrett, Charles W.; Lucas, David M.

    Principles of rural sociology and interpersonal communication provide the foundation for a study of "Gullah" culture. The Gullahs are a group of African Americans living along the southwestern U.S. coastal territory. Gullah culture began to evolve with the enslavement of African people in the Sea Islands off the coasts of South Carolina, Georgia,…

  8. The Limits of Cultural Competence: An Indigenous Studies Perspective

    Science.gov (United States)

    Carey, Michelle

    2015-01-01

    Taking the Universities Australia report, "National best practice framework for Indigenous cultural competency in Australian universities" (2011) as the starting point for its discussion, this paper examines the applicability of cultural competence in the design and delivery of Australian Indigenous Studies. It argues that both the…

  9. "Two Cultures" Topics for General Studies Science Courses.

    Science.gov (United States)

    Larson, James H.

    1982-01-01

    Theses proposed in C. P. Snow's book "The Two Cultures," including uncommunicative scientific and literary groups, gap between rich and poor, overpopulation, and nuclear war remain viable topics. Discusses the scientific and literary cultural gap and what can be done in general studies science courses to ameliorate the condition.…

  10. "Two Cultures" Topics for General Studies Science Courses.

    Science.gov (United States)

    Larson, James H.

    1982-01-01

    Theses proposed in C. P. Snow's book "The Two Cultures," including uncommunicative scientific and literary groups, gap between rich and poor, overpopulation, and nuclear war remain viable topics. Discusses the scientific and literary cultural gap and what can be done in general studies science courses to ameliorate the condition.…

  11. The Culturally Responsive Teacher Preparedness Scale: An Exploratory Study

    Science.gov (United States)

    Hsiao, Yun-Ju

    2015-01-01

    The purpose of this study was to investigate the competencies of culturally responsive teaching and construct a Culturally Responsive Teacher Preparedness Scale (CRTPS) for the use of teacher preparation programs and preservice teachers. Competencies listed in the scale were identified through literature reviews and input from experts. The…

  12. Quantitative study of formation of free I2 molecules in gamma-irradiated microcrystalline powders of pure and doped potassium iodide

    Science.gov (United States)

    Shirke, A. K.; Band, A. M.; Pode, R. B.; Deshmukh, B. T.

    1993-11-01

    Photodecomposition of pure and doped KI powders [KI:Cs, KI:Rb, KI:Na, KI:CI, KI:Br, KI:RbCl, KI:RbBr, KI:NaCl, impurity concentration 500 p.p.m.] to produce free I2 molecules during gamma irradiation is studied quantitatively by finding G values with the help of absorption measurements and calibration plots. Absorption measurements are made on solutions of irradiated powders after being dissolved in distilled water. G-value or Radiation chemical yield is the fundamental quantitative characteristic of radiation induced chemical change and it is defined as number of ions, free radicals, atoms or molecules formed or destroyed when the system has absorbed 100 eV of energy. G(I2) {Rate of formation of free I2 molecules} for anion doped KI powder [KI:CI, KI:Br] is more compared to pure KI powder. G(I2) for cation doped KI powder [KI:Rb, KI:Cs, KI:Na] is less compared to pure KI powder. Comparatively, G(I2) values obtained are small in KI:RbCl, KI:RbBr and KI:NaCl where both cation and anion are different from the host. These results are explained on the basis of misfit between impurity ions and host ions (difference between ionic radii of impurity ions and host ions). As misfit increases, G(I2) increases in cation doped powders where as in anion doped powders, G(I2) increases with the decrease in misfit.

  13. The transmission and stability of cultural life scripts: a cross-cultural study.

    Science.gov (United States)

    Janssen, Steve M J; Haque, Shamsul

    2017-06-06

    Cultural life scripts are shared knowledge about the timing of important life events. In the present study, we examined whether cultural life scripts are transmitted through traditions and whether there are additional ways through which they can be attained by asking Australian and Malaysian participants which information sources they had used to generate the life script of their culture. Participants hardly reported that they had used cultural and religious traditions. They more often reported that they had used their own experiences and experiences of relatives and friends. They also reported the use of comments of relatives and friends and the use of newspapers, books, movies and television programmes. Furthermore, we examined the stability of life scripts and similarities and differences across cultures. We found that life scripts are stable cognitive structures and that there are, besides cross-cultural differences in the content, small cross-cultural differences in the valence and distribution of life script events, with the Australian life script containing more positive events and more events expected to occur before the age of 16.

  14. Pure and Public, Popular and personal

    DEFF Research Database (Denmark)

    Eriksson, Birgit

    2013-01-01

    In the article I reexamine the traditional aesthetical and political critiques of popular culture and reevaluate the social and communicative potential of bestselling cultural artifacts such as highly popular television series. First, I sketch the alleged aesthetic and social problems of popular...... and the exclusions of the public sphere. I argue that the ideals of a pure aesthetic and a public sphere neglect issues that are crucial to the type of commonality at stake in popular cultural artifacts: personal issues, social conflicts, and what is pleasurable to the senses or has to do with emotions. Third, I...

  15. Depicting favorite organizational culture: An empirical case study

    Directory of Open Access Journals (Sweden)

    Habibollah Ranaei kordshouli

    2013-11-01

    Full Text Available The purpose of this study is to develop a model to depict favorite organizational culture. The research population consists of all Mapna’s executive managers and the research sample includes 19 managers. In order to depict favorite organizational culture, in the first step, three approaches are applied and then the results of these three approaches are compared. In the first approach, Cameron and Quinn (1999 framework [Cameron, K. S., & Quinn, R. E. (2011. Diagnosing and changing organizational culture: Based on the competing values framework. John Wiley & Sons] and success indexes are used to determine favorite organizational culture. In the second approach, benchmarking technique is applied by implementing the Denison organizational culture database. In the third approach, based on competitive value framework (CVF, a questionnaire is developed and distributed among managers and the result is applied to depict favorite organizational culture. In order to analyze data, descriptive statistics are applied and the results indicate that all of these three approaches maintain the same results. Regarding to these approaches, one or all of these approaches are applicable for depicting favorite culture. Finally, the rate of gap between status quo and favorite organizational culture can be assessed and we can develop and implement plans for improving organizational culture.

  16. Determining School Administrators’ Perceptions on Institutional Culture: A Qualitative Study

    Directory of Open Access Journals (Sweden)

    Secil Eda Kartal

    2016-03-01

    Full Text Available Schools, the where educational activities are carried out, are among the major institutions society considers as important. Schools undertake strategic responsibilities in maintaining cultural values and conveying them to future generations. The primary responsibility in achieving these missions is assigned to the school administrators. The purpose of this study is to determine the perceptions of school administrators on institutional culture. This is a qualitative study conducted on school administrators who were selected based on the volunteering principle. Perceptions of school administrators concerning their institutions’ culture and the differences between their institutional culture and other institution’s cultures were determined and analyzed. Findings of this study suggest that school administrators have both positive and negative opinions regarding their institutional culture and cultural difference. While love-respect, collaboration-solidarity and common history were prominent positive opinions; lack of communication, lack of shared values and low expectation were prominent negative opinions. In addition, participants stated the environment as a crucial factor when defining culture.

  17. [Application of cell co-culture techniques in medical studies].

    Science.gov (United States)

    Luo, Yun; Sun, Gui-Bo; Qin, Meng; Yao, Fan; Sun, Xiao-Bo

    2012-11-01

    As the cell co-culture techniques can better imitate an in vivo environment, it is helpful in observing the interactions among cells and between cells and the culture environment, exploring the effect mechanisms of drugs and their possible targets and filling the gaps between the mono-layer cell culture and the whole animal experiments. In recently years, they has attracted much more attention from the medical sector, and thus becoming one of research hotspots in drug research and development and bio-pharmaceutical fields. The cell co-culture techniques, including direct and indirect methods, are mainly used for studying pathological basis, new-type treatment methods and drug activity screening. Existing cell co-culture techniques are used for more pharmacological studies on single drug and less studies on interaction of combined drugs, such as collaborative compatibility and attenuation and synergistic effect among traditional Chinese medicines (TCMs). In line with the action characteristics of multi-component and multi-target, the cell co-culture techniques provide certain reference value for future studies on the effect and mechanism of combined TCMs on organisms as well as new methods for studies on TCMs and their compounds. This essay summarizes cell co-culture methods and their application and look into the future of their application in studies on TCMs and compounds.

  18. Indigenous Cultural Self-Representation and Its Internal Critiques: A Case Study of the Woodland Cultural Centre, Canada

    Science.gov (United States)

    Nakamura, Naohiro

    2014-01-01

    This research report discusses Indigenous cultural representation and its internal critiques, based on the case study of an Indigenous-run museum, the Woodland Cultural Centre, in Canada. Since its establishment in 1972, the Woodland Cultural Centre has strived to promote Indigenous culture, especially First Nations art, and has challenged the…

  19. A study of PbTiO sub 3 crystallization in pure and composite nanopowders prepared by the sol-gel technique

    CERN Document Server

    Cernansky, M; Kral, K; Krupkova, R

    2002-01-01

    In this investigation the crystallization of PbTiO sub 3 upon annealing of pure nanopowders and PbTiO sub 3 -SiO sub 2 (1:1 v/v) nanocomposite powders prepared by the sol-gel technique was studied. Using x-ray diffraction phase analysis, the start of PbTiO sub 3 crystallization in pure PbTiO sub 3 powders was detected at 400 sup o C. Distinct crystallization of PbTiO sub 3 in PbTiO sub 3 -SiO sub 2 nanocomposites starts at 700 sup o C, whereas SiO sub 2 remains amorphous. There are indications that an interface interaction between the PbTiO sub 3 and the SiO sub 2 phase plays an important role in hindering the crystallization of PbTiO sub 3. The particle size (size of coherently scattering regions) was estimated from the broadening of the x-ray diffraction line profiles. The average size of PbTiO sub 3 nanocrystallites increases with temperature and time of annealing, the influence of temperature being more significant than that of the annealing time. Differential scanning calorimetry confirmed the results of...

  20. Comparative incidence of a first thrombotic event in purely obstetric antiphospholipid syndrome with pregnancy loss: the NOH-APS observational study.

    Science.gov (United States)

    Gris, Jean-Christophe; Bouvier, Sylvie; Molinari, Nicolas; Galanaud, Jean-Philippe; Cochery-Nouvellon, Eva; Mercier, Erik; Fabbro-Peray, Pascale; Balducchi, Jean-Pierre; Marès, Pierre; Quéré, Isabelle; Dauzat, Michel

    2012-03-15

    The incidence of thrombosis in the purely obstetric form of antiphospholipid syndrome is uncertain. We performed a 10-year observational study of 1592 nonthrombotic women who had experienced 3 consecutive spontaneous abortions before the 10th week of gestation or 1 fetal death at or beyond the 10th week of gestation. We compared the frequencies of thrombotic events among women positive for antiphospholipid Abs (n = 517), women carrying the F5 6025 or F2 rs1799963 polymorphism (n = 279), and women with negative thrombophilia screening results (n = 796). The annual rates of deep vein thrombosis (1.46%; range, 1.15%-1.82%), pulmonary embolism (0.43%; range, 0.26%-0.66%), superficial vein thrombosis (0.44%; range, 0.28%-0.68%), and cerebrovascular events (0.32%; range, 0.18%-0.53%) were significantly higher in aPLAbs women than in the other groups despite low-dose aspirin primary prophylaxis. Women carrying 1 of the 2 polymorphisms did not experience more thrombotic events than women who screened negative for thrombophilia. Lupus anticoagulant was a risk factor for unprovoked proximal and distal deep and superficial vein thrombosis and women in the upper quartile of lupus anticoagulant activity had the highest risk. Despite data suggesting that aPLAbs may induce pregnancy loss through nonthrombotic mechanisms, women with purely obstetric antiphospholipid syndrome are at risk for thrombotic complications.

  1. PURE DRIVE GT

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    在2004年奥林匹克赛事中,中国的李婷,孙甜甜取得了中国网球第一个金牌一女子双打冠军。忘记不了当时李婷挥动着她的BABOLAT(百保力)网拍Pure Drive Zylon 360°激动地拥抱着孙甜甜吵闹着,幸福地哭着的情景。

  2. Pure de Sitter Supergravity

    CERN Document Server

    Bergshoeff, Eric A; Kallosh, Renata; Van Proeyen, Antoine

    2015-01-01

    Using superconformal methods we derive an explicit de Sitter supergravity action invariant under spontaneously broken local ${\\cal N}=1$ supersymmetry. The supergravity multiplet interacts with a nilpotent goldstino multiplet. We present a complete locally supersymmetric action including the graviton and the fermionic fields, gravitino and goldstino, no scalars. In the global limit when supergravity multiplet decouples, our action reproduces the Volkov-Akulov theory. In the unitary gauge where goldstino vanishes we recover pure supergravity with the positive cosmological constant. The classical equations of motion, with all fermions vanishing, have a maximally symmetric solution: de Sitter space.

  3. Use of fine needle aspirate from peripheral nerves of pure-neural leprosy for cytology and PCR to confirm the diagnosis: A pilot study

    Directory of Open Access Journals (Sweden)

    Abu Hena Hasanoor Reja

    2013-01-01

    Full Text Available Background: The diagnosis of pure neural leprosy (PNL remained subjective because of over-dependence of clinical expertise and a lack of simple yet reliable diagnostic tool. The criteria for diagnosis, proposed by Jardim et al., are not routinely done by clinicians in developing country as it involves invasive nerve biopsy and sophisticated anti-PGL-1 detection. We conducted a study using fine needle aspiration cytology (FNAC coupled with Ziehl Neelsen staining (ZN staining and Multiplex- Polymerase Chain Reaction (PCR specific for M. leprae for an objective diagnosis of pure neural leprosy (PNL, which may be simpler and yet reliable. Aim: The aim of the study is to couple FNAC with ZN staining and multiplex PCR to diagnose pure neural leprosy patients rapidly, in simpler and yet reliable way. Methods: Thirteen patients of PNL as diagnosed by two independent consultants were included as case, and 5 patients other than PNL were taken as control in the study. Fine needle aspiration was done on the affected nerve, and aspirates were evaluated for cytology, ZN staining and multiplex- PCR. Results: Out of the 13 cases where fine needle aspiration was done, M. leprae could be elicited in the nerve tissue aspirates in 5 cases (38.4% with the help of conventional acid-fast staining and 11 cases (84.6% with the help of multiplex PCR. On cytological examination of the aspirates, only 3 (23% cases showed specific epithelioid cells, whereas 8 (61.5% cases showed non-specific inflammation, and 2 (15.3% cases had no inflammatory cells. Conclusion: Our study demonstrates that in the field of laboratory diagnosis of PNL cases, FNAC in combination with ZN staining for acid-fast bacilli (AFB and Multiplex-PCR can provide a rapid and definitive diagnosis for the majority of PNL cases. FNAC is a less-invasive, outdoor-based and simpler technique than invasive nerve biopsy procedure. Thus, this study may enlighten the future path for easy and reliable diagnosis of

  4. Cultural Studies og kritisk diskursanalyse i 8. klasse

    DEFF Research Database (Denmark)

    Svarstad, Lone

    2015-01-01

    The article presents an exploration of a Cultural Studies and Critical Cultural Pedagogy approach to intercultural competence in lower secondary English classes in Denmark. The presentation is based on the second intervention in a participatory action research project in connection with my doctoral...... thesis in the school year 2013-2014.The approach is based on Cultural Studies and knowledge of social categories and representation, and how these can be taught and discussed in class through an intersectional lens. An intersectional lens allows for the conceptualization of categories...

  5. Health communication: a media and cultural studies approach

    National Research Council Canada - National Science Library

    Lewis, Belinda; Lewis, Jeff

    2015-01-01

    "This book is about communicating for health and social change. With a clear focus on public health and health promotion practice, it provides a unique introduction to media and cultural studies perspectives on health communication...

  6. After Progressivism: Modern Composition, Institutional Service, and Cultural Studies.

    Science.gov (United States)

    Murphy, Michael

    1993-01-01

    Outlines the historical development of composition theory as autonomous field and its relation to surrounding academic and social situations, especially progressivism. Discusses composition as a "service" course. Critiques recent alliances between composition and cultural studies theory. (HB)

  7. A study on relationship between organizational culture and organizational commitment

    Directory of Open Access Journals (Sweden)

    Maryam Khalili

    2014-07-01

    Full Text Available This paper presents an empirical investigation to study the relationship between organizational culture and organization commitment. The study uses two questionnaires, one for measuring organizational commitment originally developed by Meyer and Allen (1991 [Meyer, J. P., & Allen, N. J. (1991. A three-component conceptualization of organizational commitment. Human resource management review, 1(1, 61-89.] and the other one for organizational culture developed by Denison and Spreitzer (1991 [Denison, D. R., & Spreitzer, G. M. (1991. Organizational culture and organizational development: A competing values approach. Research in organizational change and development, 5(1, 1-21.]. The study is accomplished among selected full time employees who work for an Iranian bank named Bank Saderat Iran. Using Pearson correlation test as well as linear regression methods, the study has determined that there were some positive and meaningful relationship between all components of organizational commitment and organizational culture.

  8. Program for Culture and Conflict Studies, web page capture

    OpenAIRE

    Naval Postgraduate School (U.S.)

    2014-01-01

    web page capture from the NPS website The Program for Culture and Conflict Studies (CCS) is premised on the belief that the United States must understand the cultures and societies of the world to effectively interact with local people. It is dedicated to the study of anthropological, ethnographic, social, political, and economic data to inform U.S. policies at both the strategic and operational levels.

  9. Does calf muscle spasticity contribute to postural imbalance? A study in persons with pure hereditary spastic paraparesis

    NARCIS (Netherlands)

    Niet, M. de; Weerdesteyn, V.G.M.; Bot, S.T. de; Warrenburg, B.P.C. van de; Geurts, A.C.H.

    2013-01-01

    OBJECTIVES: The contribution of spasticity to postural imbalance in patients with upper motor neuron syndrome is still unclear. This study aimed to evaluate the responses to support-surface perturbations in patients with hereditary spastic paraparesis (HSP). These patients typically suffer from

  10. Does calf muscle spasticity contribute to postural imbalance? A study in persons with pure hereditary spastic paraparesis

    NARCIS (Netherlands)

    Niet, M. de; Weerdesteyn, V.G.M.; Bot, S.T. de; Warrenburg, B.P.C. van de; Geurts, A.C.H.

    2013-01-01

    OBJECTIVES: The contribution of spasticity to postural imbalance in patients with upper motor neuron syndrome is still unclear. This study aimed to evaluate the responses to support-surface perturbations in patients with hereditary spastic paraparesis (HSP). These patients typically suffer from bila

  11. Evaluation of unique identifiers used as keys to match identical publications in Pure and SciVal - a case study from health science.

    Science.gov (United States)

    Madsen, Heidi Holst; Madsen, Dicte; Gauffriau, Marianne

    2016-01-01

    Unique identifiers (UID) are seen as an effective key to match identical publications across databases or identify duplicates in a database. The objective of the present study is to investigate how well UIDs work as match keys in the integration between Pure and SciVal, based on a case with publications from the health sciences. We evaluate the matching process based on information about coverage, precision, and characteristics of publications matched versus not matched with UIDs as the match keys. We analyze this information to detect errors, if any, in the matching process. As an example we also briefly discuss how publication sets formed by using UIDs as the match keys may affect the bibliometric indicators number of publications, number of citations, and the average number of citations per publication.  The objective is addressed in a literature review and a case study. The literature review shows that only a few studies evaluate how well UIDs work as a match key. From the literature we identify four error types: Duplicate digital object identifiers (DOI), incorrect DOIs in reference lists and databases, DOIs not registered by the database where a bibliometric analysis is performed, and erroneous optical or special character recognition. The case study explores the use of UIDs in the integration between the databases Pure and SciVal. Specifically journal publications in English are matched between the two databases. We find all error types except erroneous optical or special character recognition in our publication sets. In particular the duplicate DOIs constitute a problem for the calculation of bibliometric indicators as both keeping the duplicates to improve the reliability of citation counts and deleting them to improve the reliability of publication counts will distort the calculation of average number of citations per publication. The use of UIDs as a match key in citation linking is implemented in many settings, and the availability of UIDs may become

  12. Evaluation of unique identifiers used as keys to match identical publications in Pure and SciVal – a case study from health science

    Science.gov (United States)

    Madsen, Heidi Holst; Madsen, Dicte; Gauffriau, Marianne

    2016-01-01

    Unique identifiers (UID) are seen as an effective key to match identical publications across databases or identify duplicates in a database. The objective of the present study is to investigate how well UIDs work as match keys in the integration between Pure and SciVal, based on a case with publications from the health sciences. We evaluate the matching process based on information about coverage, precision, and characteristics of publications matched versus not matched with UIDs as the match keys. We analyze this information to detect errors, if any, in the matching process. As an example we also briefly discuss how publication sets formed by using UIDs as the match keys may affect the bibliometric indicators number of publications, number of citations, and the average number of citations per publication.  The objective is addressed in a literature review and a case study. The literature review shows that only a few studies evaluate how well UIDs work as a match key. From the literature we identify four error types: Duplicate digital object identifiers (DOI), incorrect DOIs in reference lists and databases, DOIs not registered by the database where a bibliometric analysis is performed, and erroneous optical or special character recognition. The case study explores the use of UIDs in the integration between the databases Pure and SciVal. Specifically journal publications in English are matched between the two databases. We find all error types except erroneous optical or special character recognition in our publication sets. In particular the duplicate DOIs constitute a problem for the calculation of bibliometric indicators as both keeping the duplicates to improve the reliability of citation counts and deleting them to improve the reliability of publication counts will distort the calculation of average number of citations per publication. The use of UIDs as a match key in citation linking is implemented in many settings, and the availability of UIDs may become

  13. Microfluidics co-culture systems for studying tooth innervation

    Directory of Open Access Journals (Sweden)

    Pierfrancesco ePagella

    2014-08-01

    Full Text Available Innervation plays a key role in the development and homeostasis of organs and tissues of the orofacial complex. Among these structures, teeth are peculiar organs as they are not innervated until later stages of development. Furthermore, the implication of neurons in tooth initiation, morphogenesis and differentiation is still controversial. Co-cultures constitute a valuable method to investigate and manipulate the interactions of nerve fibres with their target organs in a controlled and isolated environment. Conventional co-cultures between neurons and their target tissues have already been performed, but these cultures do not offer optimal conditions that are closely mimicking the in vivo situation. Indeed, specific cell populations require different culture media in order to preserve their physiological properties. In this study we evaluate the usefulness of a microfluidics system for co-culturing mouse trigeminal ganglia and developing teeth. This device allows the application of specific media for the appropriate development of both neuronal and dental tissues. The results show that mouse trigeminal ganglia and teeth survive for long culture periods in this microfluidics system, and that teeth maintain the attractive or repulsive effect on trigeminal neurites that has been observed in vivo. Neurites are repealed when co-cultured with embryonic tooth germs, while postnatal teeth exert an attractive effect to trigeminal ganglia-derived neurons.In conclusion, microfluidics system devices provide a valuable tool for studying the behaviour of neurons during the development of orofacial tissues and organs, faithfully imitating the in vivo situation.

  14. Researching measurement equivalence in cross-cultural studies

    Directory of Open Access Journals (Sweden)

    Miloš Kankaraš

    2010-06-01

    Full Text Available In cross-cultural comparative studies it is essential to establish equivalent measurement of relevant constructs across cultures. If this equivalence is not confirmed it is difficult if not impossible to make meaningful comparison of results across countries. This work presents concept of measurement equivalence, its relationship with other related concepts, different equivalence levels and causes of inequivalence in cross-cultural research. It also reviews three main approaches to the analysis of measurement equivalence – multigroup confirmatory factor analysis, differential item functioning, and multigroup latent class analysis – with special emphasis on their similarities and differences, as well as comparative advantages.

  15. Safety Culture Enhancement Project. Final Report. A Field Study on Approaches to Enhancement of Safety Culture

    Energy Technology Data Exchange (ETDEWEB)

    Lowe, Andrew; Hayward, Brent (Dedale Asia Pacific, Albert Park VIC 3206 (Australia))

    2006-08-15

    This report documents a study with the objective of enhancing safety culture in the Swedish nuclear power industry. A primary objective of this study was to ensure that the latest thinking on human factors principles was being recognised and applied by nuclear power operators as a means of ensuring optimal safety performance. The initial phase of the project was conducted as a pilot study, involving the senior management group at one Swedish nuclear power-producing site. The pilot study enabled the project methodology to be validated after which it was repeated at other Swedish nuclear power industry sites, providing a broad-ranging analysis of opportunities across the industry to enhance safety culture. The introduction to this report contains an overview of safety culture, explains the background to the project and sets out the project rationale and objectives. The methodology used for understanding and analysing the important safety culture issues at each nuclear power site is then described. This section begins with a summary of the processes used in the information gathering and data analysis stage. The six components of the Management Workshops conducted at each site are then described. These workshops used a series of presentations, interactive events and group exercises to: (a) provide feedback to site managers on the safety culture and safety leadership issues identified at their site, and (b) stimulate further safety thinking and provide 'take-away' information and leadership strategies that could be applied to promote safety culture improvements. Section 3, project Findings, contains the main observations and output from the project. These include: - a brief overview of aspects of the local industry operating context that impinge on safety culture; - a summary of strengths or positive attributes observed within the safety culture of the Swedish nuclear industry; - a set of identified opportunities for further improvement; - the aggregated

  16. Reusability study of Novozym® 435 for the enzymatic synthesis of mannosyl myristate in pure ionic liquids

    Directory of Open Access Journals (Sweden)

    Galonde, N.

    2013-01-01

    Full Text Available When developing a biocatalyzed synthesis route, the enzyme reusability is an important parameter to consider for the reduction of industrial costs. In this context, the functional stability of Novozym® 435 in ionic liquids (ILs was studied in the transesterification of mannose with vinyl myristate. The enzyme was re-used five times in three ILs, 1-butyl-3-methylimidazolium tetrafluoroborate ([Bmim][BF4], 1-butyl-3-methylimidazolium trifluoromethanesulfonate ([Bmim][TFO], 1-butyl-1-methylpyrrolidinium trifluoromethanesulfonate ([Bmpyrr][TFO] and in tert-butanol (tert-BuOH. [Bmpyrr][TFO] showed the best 24 h-yield (24 h-η, with 68.8% after the first cycle and the lowest loss of 24 h-η (42% after five cycles (24 h-η of 39.9%. In comparison with [Bmpyrr][TFO], Novozym® 435 presented the most prominent loss of activity after five cycles of reaction in [Bmim][TFO] (loss of 89%, despite the good 24 h-η obtained after one cycle (60%. [Bmim][BF4] was the least interesting IL, as it was found to lead to the lowest 24 h-η, with 24.5% after one cycle and a significant loss of activity (77% after five cycles, with a 24 h-η of 5.6%. After five cycles, the 24 h-η in [Bmpyrr][TFO] was higher than in tert-BuOH and the yield loss was higher for the organic solvent (57%. Consequently, these results reveal that, in the present study, the pyrrolidium-based IL [Bmpyrr][TFO] represented the best IL as it allowed the highest level of enzymatic activity and functional stability of Novozym® 435.

  17. Pure laparoscopic augmentation ileocystoplasty.

    Science.gov (United States)

    Rebouças, Rafael B; Monteiro, Rodrigo C; Souza, Thiago N S de; Aragão, Augusto J de; Burity, Camila R T; Nóbrega, Júlio C de A; Oliveira, Natália S C de; Abrantes, Ramon B; Dantas Júnior, Luiz B; Cartaxo Filho, Ricardo; Negromonte, Gustavo R P; Sampaio, Rafael da C R; Britto, Cesar A

    2014-01-01

    Guillain-Barre syndrome is an acute neuropathy that rarely compromises bladder function. Conservative management including clean intermittent catheterization and pharmacotherapy is the primary approach for hypocompliant contracted bladder. Surgical treatment may be used in refractory cases to improve bladder compliance and capacity in order to protect the upper urinary tract. We describe a case of pure laparoscopic augmentation ileocystoplasty in a patient affected by Guillain-Barre syndrome. A 15-year-old female, complaining of voiding dysfunction, recurrent urinary tract infection and worsening renal function for three months. A previous history of Guillain-Barre syndrome on childhood was related. A voiding cystourethrography showed a pine-cone bladder with moderate post-void residual urine. The urodynamic demonstrated a hypocompliant bladder and small bladder capacity (190 mL) with high detrusor pressure (54 cmH2O). Nonsurgical treatments were attempted, however unsuccessfully.

  18. Study on the Properties of TiN Coatings on Previously Ion-Implanted Pure Magnesium Surface by MEVVA Ion Implantation

    Institute of Scientific and Technical Information of China (English)

    ZHOU Hai; CHEN Fei; WANG Jian-ping

    2007-01-01

    A metal vapor vacuum arc (MEVVA) is used in ion implantation for substrate preparation before the deposition process which would ensure the improvement of mechanical properties of the coating.Ti ion is implanted into pure magnesium surface by MEVVA implanter operated with a modified cathode.Implanting energy is kept at 45 keV and dose is set at 3×1017 cm-2.TiN coatings are deposited by magnetically filtered vacuum-arc plasma source on unimplanted and previously implanted substrates.Microstructure and phase composition are analysed using scanning electron microscopy (SEM) and X-ray diffraction (XRD).The property of corrosion resistance of TiN coatings was studied by CS300P electrochemistry-corrosion workstation,and the main impact factor of the corrosion resistance was also analyzed.

  19. Interfacial behavior of pure surfactants for enhanced oil recovery. Part 1. A study on the adsorption and distribution of cetylbenzene sulfonate

    Energy Technology Data Exchange (ETDEWEB)

    Weihong Qiao; Liangjun Dong; Zhongkui Zhao; Jie Yang; Huamin Li; Zongshi Li [Dalian Univ. of Technology (China)

    2003-04-01

    The contents of pure alkyl benzene sulfonate surfactants in both the aqueous and oil phases were determined. The diffusion, adsorption and distribution of the surfactants in the oil and aqueous phases were studied at dynamic equilibrium and the effects of different structures on diffusion were investigated. N-nonane was used as the oil phase. The results indicate that the contents of the surfactants on both sides of the interface tend to increase with increasing sodium hydroxide and sodium chloride concentrations. The corresponding interfacial tensions, however, decrease gradually, and the quantities adsorbed at the oil-water interface reach a maximum value. The content of surfactant at the interface tends to be higher when the benzene ring is located near the middle of the carbon chain, so resulting in ultralow interfacial tension. (orig.)

  20. Magnetocrystalline anisotropy of pure magnetic semiconductors of MnGeP2 and MnGeAs2: A first-principles study

    Science.gov (United States)

    Cha, Gi-Beom; Yun, Won Seok; Hong, Soon Cheol

    2016-12-01

    In this study, we use the all-electron full-potential linearized augmented plane wave method to investigate the magnetocrystalline anisotropies of pure magnetic MnGeP2 and MnGeAs2 semiconductors. Both MnGeP2 and MnGeAs2 have been calculated to be more stable in their antiferromagnetic (AFM) states than in their ferromagnetic (FM) states, with energy differences of about 230 and 190 meV/Mn, respectively. MnGeP2 and MnGeAs2 exhibit a positive EMCA (magnetization along the z axis) of +52.3 and +122.6 μeV/Mn in their AFM magnetic ground states with a negative EMCA (magnetization on the xy plane) of -71.1 and -177.8 μeV/Mn in their FM states. The EMCA behaviors are analyzed in terms of the electronic structure.

  1. The influence of surface oxygen and hydroxyl groups on the dehydrogenation of ethylene, acetic acid and hydrogenated vinyl acetate on pure Pd(1 0 0): A DFT study

    Science.gov (United States)

    Huang, Yanping; Dong, Xiuqin; Yu, Yingzhe; Zhang, Minhua

    2016-12-01

    On the basis of a Langmuir-Hinshelwood-type mechanism, the dehydrogenation of ethylene, acetic acid and hydrogenated vinyl acetate (VAH) on pure Pd(1 0 0) with surface oxygen atoms (Os) and hydroxyl groups (OHs) was studied with density functional theory (DFT) method. Our calculation results show that both Os and OHs can consistently reduce the activation energies of dehydrogenation of ethylene, acetic acid and VAH to some degree with only one exception that OHs somehow increase the activation energy of VAH. Based on Langmuir-Hinshelwood mechanism, the three dehydrogenation reactions in presence of surface Os and OHs are almost consistently favored, compared with the corresponding processes on clean Pd(1 0 0) surfaces, and thus a Langmuir-Hinshelwood-type mechanism may not be excluded beforehand when investigating the microscopic performance of the oxygen-assisted vinyl acetate synthesis on Pd(1 0 0) catalysts.

  2. Study Abroad in Psychology: Increasing Cultural Competencies through Experiential Learning

    Science.gov (United States)

    Earnest, David R.; Rosenbusch, Katherine; Wallace-Williams, Devin; Keim, Alaina C.

    2016-01-01

    Despite the prominence of study abroad programs, few are offered in the field of psychology. The current study sought to investigate the impact of study abroad programs in psychology through a comparison of study abroad and domestic student cultural competencies. Participants included 104 undergraduate students enrolled in either a psychology…

  3. Study Abroad in Psychology: Increasing Cultural Competencies through Experiential Learning

    Science.gov (United States)

    Earnest, David R.; Rosenbusch, Katherine; Wallace-Williams, Devin; Keim, Alaina C.

    2016-01-01

    Despite the prominence of study abroad programs, few are offered in the field of psychology. The current study sought to investigate the impact of study abroad programs in psychology through a comparison of study abroad and domestic student cultural competencies. Participants included 104 undergraduate students enrolled in either a psychology…

  4. COMPARISON OF BOND STRENGTH OF COMMERCIALLY PURE TITANIUM AND NICKEL CHROMIUM ALLOY WITH THREE DIFFERENT LUTING CEMENTS: AN IN-VITRO STUDY

    Directory of Open Access Journals (Sweden)

    Lakshmi

    2016-06-01

    Full Text Available BACKGROUND Metal ceramic fixed dental prosthesis remains widely used for oral rehabilitation. The type of alloy used to fabricate the metal substructure of the crown also affects its retention. The aim of this study is to compare the bond strength of commercially pure titanium and nickel chromium plates cemented with three different cements and to comparatively evaluate the bond strength of each luting cement. METHODS Specimens of each metal were divided into three groups, which received one of the following luting techniques: Group 1 (CPTi and Group 2 (NiCr with resin cement; Group 3 (CPTi and Group 4 (NiCr with Glass Ionomer Cement; Group 5 (CPTi and Group 6 (NiCr with Zinc phosphate cement. The bonded specimens were submitted for the bond strength tests conducted with a Universal Testing Machine with a shear mode under a crosshead speed of 0.5 mm/min. Debonded specimens were examined under electron microscope. RESULT The results indicate that Group 1 and 2 have significantly higher values than Group 3, 4, 5 and 6. Also, Group 3 and 4 have significantly higher values when compared to Group 5 and 6. Whereas, there was no significant difference between Group 1 and 2, Group 3 and 4 as well as Group 5 and 6. The scanning electron microscope illustrated the different modes of fracture that occurred at the metal cement interface. Resin cement showed predominantly cohesive failure. Glass ionomer cement showed a mixed mode of both cohesive and adhesive fracture and Zinc phosphate cement also showed mixed mode of fracture with predominantly adhesive failure. CONCLUSIONS Resin cements showed the most superior bond with both commercially pure titanium and nickel chromium metal. Zinc phosphate cement showed the lowest bond strength with both the metals. There was no significant difference observed between the cement bond with different metals.

  5. Microstructural studies and wear assessments of Ti/TiC surface composite coatings on commercial pure Ti produced by titanium cored wires and TIG process

    Energy Technology Data Exchange (ETDEWEB)

    Monfared, A., E-mail: amirmonfared25@yahoo.com [Department of Materials Science and Engineering, Sharif University of Technology, Tehran (Iran, Islamic Republic of); Kokabi, A.H.; Asgari, S. [Department of Materials Science and Engineering, Sharif University of Technology, Tehran (Iran, Islamic Republic of)

    2013-01-15

    Tungsten Inert Gas (TIG) process and titanium cored wires filled with micro size TiC particles were employed to produce surface composite coatings on commercial pure Ti substrate for wear resistance improvement. Wire drawing process was utilized to produce several cored wires from titanium strips and titanium carbide powders. Subsequently, these cored wires were melted and coated on commercial pure Ti using TIG process. This procedure was repeated at different current intensities and welding travel speeds. Composite coating tracks were found to be affected by TIG heat input. The microstructural studies using optical and scanning electron microscopy supported by X-ray diffraction showed that the surface composite coatings consisted of {alpha} Prime -Ti, spherical and dendritic TiC particles. Also, greater volume fractions of TiC particles in the coatings were found at lower heat input. A maximum microhardness value of about 1100 HV was measured which is more than 7 times higher than the substrate material. Pin-on-disk wear tests exhibited a better performance of the surface composite coatings than the untreated material which was attributed to the presence of TiC particles in the microstructure. -- Highlights: Black-Right-Pointing-Pointer Ti/TiC composite coatings were produced on the CP-Ti. Black-Right-Pointing-Pointer Titanium cored wire and TIG process were employed for production of the coatings. Black-Right-Pointing-Pointer Decreasing heat input, increased the volume fraction of TiC in the coatings. Black-Right-Pointing-Pointer The maximum microhardness obtained in the lowest heat input. Black-Right-Pointing-Pointer The wear resistance of the coatings improved due to the formation of TiC particles.

  6. Organizational Culture

    Directory of Open Access Journals (Sweden)

    Adrian HUDREA

    2006-02-01

    Full Text Available Cultural orientations of an organization can be its greatest strength, providing the basis for problem solving, cooperation, and communication. Culture, however, can also inhibit needed changes. Cultural changes typically happen slowly – but without cultural change, many other organizational changes are doomed to fail. The dominant culture of an organization is a major contributor to its success. But, of course, no organizational culture is purely one type or another. And the existence of secondary cultures can provide the basis for change. Therefore, organizations need to understand the cultural environments and values.

  7. Study of Nosocomial Clostridium Difficile Diarrhea by Culture and Tissue Culture Methods

    Directory of Open Access Journals (Sweden)

    MH Salari

    2007-06-01

    Full Text Available Background: Clostridium difficult is an identified cause of antibiotic-associated diarrhea, antibiotic-associated colitis, pseudomembranous colitis and nosocomial diarrhea. The purpose of this study was to investigate the prevalence of nosocomial C. difficile-associated diarrhea in Tehran University of Medical Science Hospitals. Methods: In this study a total of 942 stool samples from patients with nosocomial diarrhea that were hospitalized in Imam Khomeini hospital, Shariati hospital and Children clinical center were collected. The samples were cultured on a selective cycloserine cefoxitin fructose agar (CCFA and incubated in anaerobic conditions, at 37 °C for 5 days. Isolated C. difficile by conventional biochemical tests, bacterial cytotoxicity by Vero tissue culture and antimicrobial sensitivity to antibiotics by Kirby Bauer method (disk diffusion were investigated. Results: Of the total patients, 57 Toxigenic C. difficile (6.1% were isolated. Results of statistical analysis show significant differences between the rate of isolated Toxigenic C. difficile and age group of patients (P< 0.05. Among the units of selected hospitals, Toxigenic C. difficile was isolated most frequently in gastroenterology of Children clinical center. Meanwhile, the isolated Toxigenic C. difficile were sensitive to vancomycin, Chloramphenicol and ceftriaxone. Conclusion: Our findings show that, Toxigenic C. difficile was found in 6.1% hospitalized patients. Therefore, further studies to evaluate the role of Toxigenic C. difficile in nosocomial diarrhea processes, ecological and pathogenic terms by culture, Tissue culture and molecular methodes are suggested.

  8. Ultrastructural study of grafted autologous cultured human epithelium.

    Science.gov (United States)

    Aihara, M

    1989-01-01

    An electron microscopical study of grafted autologous cultured human epithelium is presented. Biopsy samples were collected from four patients with full thickness burns at 9 days, 6 weeks and 5-21 months after grafting of the cultured epithelium. By the sixth week after transplantation, grafted cultured epithelial sheets had developed to consist of 10 to 20 layers of cells and the epithelium showed distinct basal, spinous, granular and horny layers, and a patchy basement membrane had formed. Langerhans cells and melanocytes were identifiable. From 5 months onwards flat basal cells became oval, and oval keratohyalin granules in the keratinocytes also assumed a normal irregular shape. Membrane-coating granules in the keratinocytes increased in number. The fine structures of desmosomes also showed a normal mature appearance. Furthermore, complete extension of the basement membrane could be observed. The maturation of cultured human epithelium is complete by 5 months after grafting.

  9. A Cross-Cultural Study of Adolescent Procrastination

    Science.gov (United States)

    Klassen, Robert M.; Ang, Rebecca P.; Chong, Wan Har; Krawchuk, Lindsey L.; Huan, Vivien S.; Wong, Isabella Y. F.; Yeo, Lay See

    2009-01-01

    In this study, we explore academic procrastination and associated motivation variables in 612 adolescents from Canada and Singapore. Few studies have explored adolescent procrastination and no previous studies have investigated adolescent procrastination using a cross-cultural framework. Singaporean adolescents reported higher levels of…

  10. Pure Laparoscopic Augmentation Ileocystoplasty

    Directory of Open Access Journals (Sweden)

    Rafael B. Rebouças

    2014-12-01

    Full Text Available Introduction Guillain-Barre syndrome is an acute neuropathy that rarely compromises bladder function. Conservative management including clean intermittent catheterization and pharmacotherapy is the primary approach for hypocompliant contracted bladder. Surgical treatment may be used in refractory cases to improve bladder compliance and capacity in order to protect the upper urinary tract. We describe a case of pure laparoscopic augmentation ileocystoplasty in a patient affected by Guillain-Barre syndrome. Presentation A 15-year-old female, complaining of voiding dysfunction, recurrent urinary tract infection and worsening renal function for three months. A previous history of Guillain-Barre syndrome on childhood was related. A voiding cystourethrography showed a pine-cone bladder with moderate post-void residual urine. The urodynamic demonstrated a hypocompliant bladder and small bladder capacity (190mL with high detrusor pressure (54 cmH2O. Nonsurgical treatments were attempted, however unsuccessfully. The patient was placed in the exaggerated Trendelenburg position. A four-port transperitoneal technique was used. A segment of ileum approximately 15-20cm was selected and divided with its pedicle. The ileal anastomosis and creation of ileal U-shaped plate were performed laparoscopically, without staplers. Bladder mobilization and longidutinal cystotomy were performed. Enterovesical anastomosis was done with continuous running suture. A suprapubic cystostomy was placed through a 5mm trocar. Results The total operative time was 335 min. The blood loss was minimal. The patient developed ileus in the early days, diet acceptance after the fourth day and was discharged on the seventh postoperative day. The urethral catheter was removed after 2 weeks. At 6-month follow-up, a cystogram showed a significant improvement in bladder capacity. The patient adhered well to clean intermittent self-catheterization and there was no report for febrile infections

  11. Safety Culture Enhancement Project. Final Report. A Field Study on Approaches to Enhancement of Safety Culture

    Energy Technology Data Exchange (ETDEWEB)

    Lowe, Andrew; Hayward, Brent (Dedale Asia Pacific, Albert Park VIC 3206 (Australia))

    2006-08-15

    This report documents a study with the objective of enhancing safety culture in the Swedish nuclear power industry. A primary objective of this study was to ensure that the latest thinking on human factors principles was being recognised and applied by nuclear power operators as a means of ensuring optimal safety performance. The initial phase of the project was conducted as a pilot study, involving the senior management group at one Swedish nuclear power-producing site. The pilot study enabled the project methodology to be validated after which it was repeated at other Swedish nuclear power industry sites, providing a broad-ranging analysis of opportunities across the industry to enhance safety culture. The introduction to this report contains an overview of safety culture, explains the background to the project and sets out the project rationale and objectives. The methodology used for understanding and analysing the important safety culture issues at each nuclear power site is then described. This section begins with a summary of the processes used in the information gathering and data analysis stage. The six components of the Management Workshops conducted at each site are then described. These workshops used a series of presentations, interactive events and group exercises to: (a) provide feedback to site managers on the safety culture and safety leadership issues identified at their site, and (b) stimulate further safety thinking and provide 'take-away' information and leadership strategies that could be applied to promote safety culture improvements. Section 3, project Findings, contains the main observations and output from the project. These include: - a brief overview of aspects of the local industry operating context that impinge on safety culture; - a summary of strengths or positive attributes observed within the safety culture of the Swedish nuclear industry; - a set of identified opportunities for further improvement; - the aggregated

  12. Academic capitalism and academic culture: A case study.

    Directory of Open Access Journals (Sweden)

    Pilar Mendoza

    2008-12-01

    Full Text Available This case study investigated the impact of academic capitalism on academic culture by examining the perspectives of faculty members in an American academic department with significant industrial funding. The results of this study indicate that faculty members believe that the broad integrity of the academic culture remains unaffected in this department and they consider industrial sponsorship as a highly effective vehicle for enhancing the quality of education of students and pursuing their scientific interests. This study provides valuable insights to federal and institutional policiescreated to foster industry-academia partnerships and commercialization of academic research.

  13. Entropy product measure for multipartite pure states

    Institute of Scientific and Technical Information of China (English)

    CAO Wancang; LIU Dan; PAN Feng; LONG Guilu

    2006-01-01

    An entanglement measure for multipartite pure states is formulated using the product of the von Neumann entropy of the reduced density matrices of the constituents.Based on this new measure, all possible ways of the maximal entanglement of the triqubit pure states are studied in detail and all types of the maximal entanglement have been culate the degree of entanglement, and an improvement is given in the area near the zero entropy.

  14. Bioaccessibility studies of ferro-chromium alloy particles for a simulated inhalation scenario: a comparative study with the pure metals and stainless steel.

    Science.gov (United States)

    Midander, Klara; de Frutos, Alfredo; Hedberg, Yolanda; Darrie, Grant; Wallinder, Inger Odnevall

    2010-07-01

    The European product safety legislation, REACH, requires that companies that manufacture, import, or use chemicals demonstrate safe use and high level of protection of their products placed on the market from a human health and environmental perspective. This process involves detailed assessment of potential hazards for various toxicity endpoints induced by the use of chemicals with a minimum use of animal testing. Such an assessment requires thorough understanding of relevant exposure scenarios including material characteristics and intrinsic properties and how, for instance, physical and chemical properties change from the manufacturing phase, throughout use, to final disposal. Temporary or permanent adverse health effects induced by particles depend either on their shape or physical characteristics, and/or on chemical interactions with the particle surface upon human exposure. Potential adverse effects caused by the exposure of metal particles through the gastrointestinal system, the pulmonary system, or the skin, and their subsequent potential for particle dissolution and metal release in contact with biological media, show significant gaps of knowledge. In vitro bioaccessibility testing at conditions of relevance for different exposure scenarios, combined with the generation of a detailed understanding of intrinsic material properties and surface characteristics, are in this context a useful approach to address aspects of relevance for accurate risk and hazard assessment of chemicals, including metals and alloys and to avoid the use of in vivo testing. Alloys are essential engineering materials in all kinds of applications in society, but their potential adverse effects on human health and the environment are very seldom assessed. Alloys are treated in REACH as mixtures of their constituent elements, an approach highly inappropriate because intrinsic properties of alloys generally are totally different compared with their pure metal components. A large

  15. CULTURAL CHANGE IN TELECOMMUNICATIONS COMPANIES: A CASE STUDY

    Directory of Open Access Journals (Sweden)

    Cristiane Alves Anacleto

    2014-09-01

    Full Text Available This work aims to propose a model for the conduct of cultural change in the IT department of a company in the telecommunications industry. To achieve the goal proposed exploratory, technical procedures used were the case study and literature review. The techniques of data collection in the case study were: participant observation, unstructured interview and document analysis. The proposed model consists of five steps: analysis of the company's strategic planning, cultural diagnosis of the IT department, brainstorming with the staff and managers, compared to the case studies surveyed and proposed actions for the conduct of cultural change. We conclude that a receptive environment, employees not only understand why the changes are necessary, but engage with the process and faithfully executes the steps required, favoring the strategic positioning of the company.

  16. Rehabilitation of pure alexia: A review

    Science.gov (United States)

    Starrfelt, Randi; Ólafsdóttir, Rannveig Rós; Arendt, Ida-Marie

    2013-01-01

    Acquired reading problems caused by brain injury (alexia) are common, either as a part of an aphasic syndrome, or as an isolated symptom. In pure alexia, reading is impaired while other language functions, including writing, are spared. Being in many ways a simple syndrome, one would think that pure alexia was an easy target for rehabilitation efforts. We review the literature on rehabilitation of pure alexia from 1990 to the present, and find that patients differ widely on several dimensions, such as alexia severity and associated deficits. Many patients reported to have pure alexia in the reviewed studies, have associated deficits such as agraphia or aphasia and thus do not strictly conform to the diagnosis. Few studies report clear and generalisable effects of training, none report control data, and in many cases the reported findings are not supported by statistics. We can, however, tentatively conclude that Multiple Oral Re-reading techniques may have some effect in mild pure alexia where diminished reading speed is the main problem, while Tacile-Kinesthetic training may improve letter identification in more severe cases of alexia. There is, however, still a great need for well-designed and controlled studies of rehabilitation of pure alexia. PMID:23808895

  17. Developing Cultural Awareness In Foreign Language Teaching

    OpenAIRE

    2006-01-01

    Regardless of different points of view, culture has taken an important place in foreign language teaching and learning studies. It has been widely recognized that culture and language is used as a main medium through which culture is expressed. However, “pure information” is useful but does not necessarily lead learners’ insight; whereas the development of people’s cultural awareness leads them to more critical thinking. Most frequently confronted that students to a great extend know the rul...

  18. Selling the Internet: A Cultural Studies Approach to Public Relations.

    Science.gov (United States)

    Mickey, Thomas J.

    1998-01-01

    Examines the ideology of Internet public relations. Takes a cultural studies approach to modern public relations, using public relations material as representation. Presents a case study of the MassNetworks Education Partnership, which was set up in 1996 to ensure that the Internet gets into the classroom. (PA)

  19. Organizational Mavericks and Innovation: A Triangulation Study of Culture.

    Science.gov (United States)

    Ugbah, Steve; And Others

    A study examined the communicative behavior of mavericks as they relate to innovation in organizations and the impact of such non-conforming behavior on organizational change. Two approaches were used: (1) studying organizational life from the cultural perspective and (2) collecting both qualitative and quantitative data. Subjects, 32 employees of…

  20. Study Tours and the Diversification of Cultural Capital Acquisition

    Science.gov (United States)

    Slotkin, Michael H.; Vamosi, Alexander R.; Perez, Enrique M.; Durie, Christopher J.; Eisenberg, Jarin R.

    2016-01-01

    Purpose: This paper aims to provide evidence on the role study tours play in expanding student cultural capital via increased confidence in international travel. Design/methodology/approach: In this paper, survey data from individuals who participated in a study tour experience offered by a Florida-based university are analyzed for the assessment…

  1. A Comparative Study between Chinese and Western Food Culture in Cross-cultural Communication

    Institute of Scientific and Technical Information of China (English)

    焦体霞

    2014-01-01

    The differences of food culture play an important role in cross-cultural communication. Learn the cultural rooted causes of food culture between Chinese and Western countries, will promote mutual understanding between people and enjoy different feelings different foods brings, enhance cultural exchange, complement and integration.

  2. CO Gas Sensing Properties of Pure and Cu-Incorporated SnO2 Nanoparticles: A Study of Cu-Induced Modifications

    OpenAIRE

    Tangirala Venkata Krishna Karthik; María de la Luz Olvera; Arturo Maldonado; Heberto Gómez Pozos

    2016-01-01

    Pure and copper (Cu)-incorporated tin oxide (SnO2) pellet gas sensors with characteristics provoking gas sensitivity were fabricated and used for measuring carbon monoxide (CO) atmospheres. Non-spherical pure SnO2 nano-structures were prepared by using urea as the precipitation agent. The resultant SnO2 powders were ball milled and incorporated with a transition metal, Cu, via chemical synthesis method. The incorporation is confirmed by high-resolution transmission electron microscope (HRTEM)...

  3. Theoretical study of oxygen adsorption on pure Au-n+1(+) and doped MAun+ cationic gold clusters for M = Ti, Fe and n=3-7

    DEFF Research Database (Denmark)

    Torres, M. Begona; Fernandez Sanchez, Eva; Balbas, Luis C.

    2008-01-01

    A comparative study of the adsorption of an O-2 molecule on pure Au-n+1(+) and doped MAun+ cationic gold clusters for n = 3-7 and M = Ti, Fe is presented. The simultaneous adsorption of two oxygen atoms also was studied. This work was performed by means of first principles calculations based...... with adsorption energies of 0.56 and 0.69 eV, respectively. The ground-state geometry of Au-n(+) is almost unperturbed after O-2 adsorption. The electronic charge flows towards O-2 when the molecule is adsorbed in bridge positions and towards the gold cluster when O-2 is adsorbed on top of An atoms, and both...... the adsorption energy and the O-O bond length of adsorbed oxygen increase when the amount of electronic charge on O-2 increases. On the other hand, we studied the adsorption of an O-2 molecule on doped MAun+ clusters, leading to the formation of (MAunO2+)(ad) complexes with different equilibrium configurations...

  4. Arsenic Removal from Aqueous Solution Using Pure and Metal-Doped Titania Nanoparticles Coated on Glass Beads: Adsorption and Column Studies

    Directory of Open Access Journals (Sweden)

    M. Ihsan Danish

    2013-01-01

    Full Text Available Nanosized metal oxide, Titania, provides high surface area and specific affinity for the adsorption of heavy metals, including arsenic (As, which is posing a great threat to the world population due to its carcinogenic nature. In this study, As(III adsorption was studied on pure and metal- (Ag- and Fe- doped Titania nanoparticles. The nanoparticles were synthesized by liquid impregnation method with some modifications, with crystallite size in the range of 30 to 40 nm. Band gap analysis, using Kubelka-Munk function showed a shift of absorption band from UV to visible region for the metal-doped Titania. Effect of operational parameters like dose of nanoparticles, initial As(III concentration, and pH was evaluated at 25°C. The data obtained gave a good fit with Langmuir and Freundlich isotherms and the adsorption was found to conform to pseudo-second-order kinetics. In batch studies, over 90% of arsenic removal was observed for both types of metal-doped Titania nanoparticles from a solution containing up to 2 ppm of the heavy metal. Fixed bed columns of nanoparticles, coated on glass beads, were used for As(III removal under different operating conditions. Thomas and Yoon-Nelson models were applied to predict the breakthrough curves and to find the characteristic column parameters useful for process design. The columns were regenerated using 10% NaOH solution.

  5. Studies of In Vitro Embryo Culture of Guppy (Poecilia reticulata).

    Science.gov (United States)

    Liu, LiLi; Lee, Ki-Young

    2014-09-01

    Different with other fishes, the guppies (Poecilia reticulata) is ovoviviparity, which retain their fertilized eggs within the follicle throughout gestation. The synchronously growing diplotene oocytes store nutrients in droplets and yolk, before their maturation and fertilization. The lecithotrophic strategy of development entails the provisioning of embryos with resources from the maternal yolk deposit rather than from a placenta, it allows the extracorporeal culture of guppy embryo. Studies on their early development of live bearers like the guppy including lineage tracing and genetic manipulations, have been limited. Therefore, to optimize conditions of embryo in vitro culture, explanted embryos from pregnant females were incubated in embryo medium (L-15 medium, supplemented with 5, 10, 15, 20% fetal bovine serum, respectively). We investigated whether the contents of FBS in vitro culture medium impact the development of embryos, and whether they would hatch in vitro. Our study found that in 5% of FBS of the medium, although embryos developed significantly slower in vitro than in the ovary, it was impossible to exactly quantify the developmental delay in culture, due to the obvious spread in developmental stage within each batch of eggs, and embryos can only be maintained until the early-eyed. And although in culture with 20% FBS the embryos can sustain rapid development of early stage, but cannot be cultured for the entire period of their embryonic development and ultimately died. In the medium with 10% and 15% FBS, the embryos seems well developed, even some can continue to grow after follicle ruptures until it can be fed. We also observed that embryonic in these two culture conditions were significantly different in development speed, in 15% it is faster than 10%. But 10% FBS appears to be more optimizing condition than 15% one on development process of embryos and survival rate to larvae stage.

  6. A study on relationship between organizational culture and communication apprehension

    Directory of Open Access Journals (Sweden)

    Aliakbar Farhangi

    2013-07-01

    Full Text Available This paper presents a study to investigate the relationship between organizational culture and communication apprehension among some employees who worked for Iran broadcasting channel. The study has adopted two well-known questionnaires developed by Hofstede (1984 [Hofstede, G. (1984. Culture's Consequences: International Differences in Work-Related Values (2nd ed.. Beverly Hills CA: SAGE Publications. ISBN 0-8039-1444-X] for organizational culture and McCroskey et al. (1985 [McCroskey, J. C., Beatty, M. J., Kearney, P., & Plax, T. G. (1985. The content validity of the PRCA‐24 as a measure of communication apprehension across communication contexts. Communication Quarterly, 33(3, 165-173.] for communication apprehension (CA. We have used Chi-Square test to verify different hypotheses of the survey and the results of the survey have indicated that while there was no relationship between CA and three components including cultures of masculinity, individualistic culture and ambiguity aversion, there was a significance relationship between CA and power distance. The results of our survey indicate that as the power distance increases we may expect a higher level on CA.

  7. Geomorphology's role in the study of weathering of cultural stone

    Science.gov (United States)

    Pope, Gregory A.; Meierding, Thomas C.; Paradise, Thomas R.

    2002-10-01

    Great monumental places—Petra, Giza, Angkor, Stonehenge, Tikal, Macchu Picchu, Rapa Nui, to name a few—are links to our cultural past. They evoke a sense of wonderment for their aesthetic fascination if not for their seeming permanence over both cultural and physical landscapes. However, as with natural landforms, human constructs are subject to weathering and erosion. Indeed, many of our cultural resources suffer from serious deterioration, some natural, some enhanced by human impact. Groups from the United Nations to local civic and tourism assemblies are deeply interested in maintaining and preserving such cultural resources, from simple rock art to great temples. Geomorphologists trained in interacting systems, process and response to thresholds, rates of change over time, and spatial variation of weathering processes and effects are able to offer insight into how deterioration occurs and what can be done to ameliorate the impact. Review of recent literature and case studies presented here demonstrate methodological and theoretical advances that have resulted from the study of cultural stone weathering. Because the stone was carved at a known date to a "baseline" or zero-datum level, some of the simplest methods (e.g., assessing surface weathering features or measuring surface recession in the field) provide useful data on weathering rates and processes. Such data are difficult or impossible to obtain in "natural" settings. Cultural stone weathering studies demonstrate the importance of biotic and saline weathering agents and the significance of weathering factors such as exposure (microclimate) and human impact. More sophisticated methods confirm these observations, but also reveal discrepancies between field and laboratory studies. This brings up two important caveats for conservators and geomorphologists. For the conservator, are laboratory and natural setting studies really analogous and useful for assessing stone damage? For the geomorphologist, does

  8. Study of sterilization-treatment in pure and N- doped carbon thin films synthesized by inductively coupled plasma assisted pulsed-DC magnetron sputtering

    Science.gov (United States)

    Javid, Amjed; Kumar, Manish; Han, Jeon Geon

    2017-01-01

    Electrically-conductive nanocrystalline carbon films, having non-toxic and non-immunogenic characteristics, are promising candidates for reusable medical devices. Here, the pure and N- doped nanocrystalline carbon films are deposited by the assistance of inductively coupled plasma (ICP) in an unbalanced facing target pulsed-DC magnetron sputtering process. Through the optical emission spectroscopy study, the role of ICP assistance and N-doping on the reactive components/radicals during the synthesis is presented. The N-doping enhances the three fold bonding configurations by increasing the ionization and energies of the plasma species. Whereas, the ICP addition increases the plasma density to control the deposition rate and film structure. As a result, sputtering-throughput (deposition rate: 31-55 nm/min), electrical resistivity (4-72 Ωcm) and water contact angle (45.12°-54°) are significantly tailored. Electric transport study across the surface microchannel confirms the superiority of N-doped carbon films for sterilization stability over the undoped carbon films.

  9. Reference ranges of handgrip strength from 125,462 healthy adults in 21 countries: a prospective urban rural epidemiologic (PURE) study

    Science.gov (United States)

    Teo, Koon K.; Rangarajan, Sumathy; Kutty, V. Raman; Lanas, Fernando; Hui, Chen; Quanyong, Xiang; Zhenzhen, Qian; Jinhua, Tang; Noorhassim, Ismail; AlHabib, Khalid F; Moss, Sarah J.; Rosengren, Annika; Akalin, Ayse Arzu; Rahman, Omar; Chifamba, Jephat; Orlandini, Andrés; Kumar, Rajesh; Yeates, Karen; Gupta, Rajeev; Yusufali, Afzalhussein; Dans, Antonio; Avezum, Álvaro; Lopez‐Jaramillo, Patricio; Poirier, Paul; Heidari, Hosein; Zatonska, Katarzyna; Iqbal, Romaina; Khatib, Rasha; Yusuf, Salim

    2016-01-01

    Abstract Background The measurement of handgrip strength (HGS) has prognostic value with respect to all‐cause mortality, cardiovascular mortality and cardiovascular disease, and is an important part of the evaluation of frailty. Published reference ranges for HGS are mostly derived from Caucasian populations in high‐income countries. There is a paucity of information on normative HGS values in non‐Caucasian populations from low‐ or middle‐income countries. The objective of this study was to develop reference HGS ranges for healthy adults from a broad range of ethnicities and socioeconomically diverse geographic regions. Methods HGS was measured using a Jamar dynamometer in 125,462 healthy adults aged 35‐70 years from 21 countries in the Prospective Urban Rural Epidemiology (PURE) study. Results HGS values differed among individuals from different geographic regions. HGS values were highest among those from Europe/North America, lowest among those from South Asia, South East Asia and Africa, and intermediate among those from China, South America, and the Middle East. Reference ranges stratified by geographic region, age, and sex are presented. These ranges varied from a median (25th–75th percentile) 50 kg (43–56 kg) in men 60 years from South East Asia. Reference ranges by ethnicity and body‐mass index are also reported. Conclusions Individual HGS measurements should be interpreted using region/ethnic‐specific reference ranges. PMID:27104109

  10. Potential of the J-PET detector for studies of discrete symmetries in decays of positronium atom - a purely leptonic system

    CERN Document Server

    Moskal, P; Bednarski, T; Białas, P; Czerwiński, E; Curceanu, C; Gajos, A; Głowacz, B; Gorgol, M; Hiesmayr, B C; Jasińska, B; Kamińska, D; Korcyl, G; Kowalski, P; Kozik, T; Krzemień, W; Krawczyk, N; Kubicz, E; Mohammed, M; Niedźwieckia, Sz; Pawlik-Niedźwiecka, M; Raczyński, L; Rudy, Z; Silarski, M; Wieczorek, A; Wiślicki, W; Zieliński, M

    2016-01-01

    The Jagiellonian Positron Emission Tomograph (J-PET) was constructed as a prototype of the cost-effective scanner for the simultaneous metabolic imaging of the whole human body. Being optimized for the detection of photons from the electron-positron annihilation with high time- and high angular-resolution, it constitutes a multi-purpose detector providing new opportunities for studying the decays of positronium atoms. Positronium is the lightest purely leptonic object decaying into photons. As an atom bound by a central potential it is a parity eigenstate, and as an atom built out of an electron and an anti-electron it is an eigenstate of the charge conjugation operator. Therefore, the positronium is a unique laboratory to study discrete symmetries whose precision is limited in principle by the effects due to the weak interactions expected at the level of (~10$^{-14}$) and photon-photon interactions expected at the level of (~10$^{-9}$). The J-PET detector enables to perform tests of discrete symmetries in th...

  11. Student nurses' experiences of living and studying in a different culture to their own and the development of cultural sensitivity

    DEFF Research Database (Denmark)

    Ruddock, Heidi

    With the increase of culturally diverse people residing in Denmark, it has become imperative to provide student nurses with knowledge and skills that will enable them to become culturally sensitive in order interact effectively with clients from culturally diverse backgrounds. The aim of this study...... was to explore whether student nurses develop cultural sensitivity as a consequence of living and studying in a culture that is different from their own. Seven Danish student nurses who had participated in student exchanges in Jamaica, Australia, Malta and Greenland took part in this study. A qualitative...... characteristics of openness and flexibility and support networks facilitated the students transition and adjustment to the host culture. Reflection on their experiences with students from a similar background to themselves and clinical mentors from the host culture assisted the students in their understanding...

  12. A comparative study about learning styles preferences of two cultures

    Science.gov (United States)

    Kutay, Huban

    From an anthropologist's (Maddock, 1981) point of view, "science and science education are cultural enterprises which form a part of the wider cultural matrix of society and educational considerations concerning science must be made in the light of this wider perspective" (p.10). In addition, Spindler (1987) states that teaching science is considered cultural transmission while, Wolcott (1991) focuses on learning science as culture acquisition. In these statements, culture is defined as "an ordered system of meaning and symbols, in terms of which social interaction takes place" (Geertz, 1973). Thus, learning and culture are a partnership. Jones and Fennimore (1990) state: "Every culture brings habits of thought, resources, and context, which have built into them vehicles that promote learning and inquiry. Accordingly, children of any culture can and should have curriculum and instructional practices that draw from that culture." (p.16). Unfortunately, even though this statement is probably accurate, most schools still use the same curriculum, instructional methods, and assessment strategies for all students regardless of their differences in learning styles. The purpose of this study is to identify the relationship between students' learning styles and their culture. This is a correlational study. Does culture limit or expand the learning of individuals? For this study Turkish students who graduated from a Turkish high school and undertook undergraduate education in the United States or finished their undergraduate education in Turkey and pursued graduate education in the United States were identified. These Turkish subjects were compared to American college students through learning styles data and anxiety levels as cultural markers. To identify individuals' learning styles we used the Building Excellence (BE) instrument, which is an adult version of The Learning Style Inventory by Dunn, and Rundle (1996.1997, 1998, 1999, and 2000). BE assesses twenty four

  13. Characterizing parameters of Jatropha curcas cell cultures for microgravity studies

    Science.gov (United States)

    Vendrame, Wagner A.; Pinares, Ania

    2013-06-01

    Jatropha (Jatropha curcas) is a tropical perennial species identified as a potential biofuel crop. The oil is of excellent quality and it has been successfully tested as biodiesel and in jet fuel mixes. However, studies on breeding and genetic improvement of jatropha are limited. Space offers a unique environment for experiments aiming at the assessment of mutations and differential gene expression of crops and in vitro cultures of plants are convenient for studies of genetic variation as affected by microgravity. However, before microgravity studies can be successfully performed, pre-flight experiments are necessary to characterize plant material and validate flight hardware environmental conditions. Such preliminary studies set the ground for subsequent spaceflight experiments. The objectives of this study were to compare the in vitro growth of cultures from three explant sources (cotyledon, leaf, and stem sections) of three jatropha accessions (Brazil, India, and Tanzania) outside and inside the petriGAP, a modified group activation pack (GAP) flight hardware to fit petri dishes. In vitro jatropha cell cultures were established in petri dishes containing a modified MS medium and maintained in a plant growth chamber at 25 ± 2 °C in the dark. Parameters evaluated were surface area of the explant tissue (A), fresh weight (FW), and dry weight (DW) for a period of 12 weeks. Growth was observed for cultures from all accessions at week 12, including subsequent plantlet regeneration. For all accessions differences in A, FW and DW were observed for inside vs. outside the PetriGAPs. Growth parameters were affected by accession (genotype), explant type, and environment. The type of explant influenced the type of cell growth and subsequent plantlet regeneration capacity. However, overall cell growth showed no abnormalities. The present study demonstrated that jatropha in vitro cell cultures are suitable for growth inside PetriGAPs for a period of 12 weeks. The parameters

  14. Studying Mobile Media Cultural Technologies, Mobile Communication, and the IPhone

    CERN Document Server

    Hjorth, Larissa; Richardson, Ingrid

    2012-01-01

    The iPhone represents an important moment in both the short history of mobile media and the long history of cultural technologies. Like the Walkman of the 1980s, it marks a juncture in which notions about identity, individualism, lifestyle and sociality require rearticulation. this book explores not only the iPhone's particular characteristics, uses and "affects," but also how the "iPhone moment" functions as a barometer for broader patterns of change. In the iPhone moment, this study considers the convergent trajectories in the evolution of digital and mobile culture, and

  15. Culture Studies in the Field of International Business Research

    DEFF Research Database (Denmark)

    Worm, Verner; Li, Xin; Jakobsen, Michael

    Purpose: The purpose of this paper is to describe the status of culture studies within the field of international business research, and to examine how two main paradigms – essentialism and social constructivism – relate to the discourse in this field. We analyze the main points of the two...... in this paper. Practical implications: We encourage practitioners to learn how to switch, both sequentially and spatially, between the two paradigms of culture (fundamentally incommensurable though they are). This involves taking a “both/or” approach to the two paradigms. Originality/Value: We show...

  16. Instituting Cultural Change at a Major Organization: A Case Study

    Science.gov (United States)

    Dulek, Ronald E.

    2015-01-01

    This article examines the development and implementation of a strategic cultural change program from a case study perspective. Initially, the article describes how the program was developed, including an explanation as to how a communication component was integrated into the program from inception. This integration helped reduce the anxiety that…

  17. Cultural Competence and School Counselor Training: A Collective Case Study

    Science.gov (United States)

    Nelson, Judith A.; Bustamante, Rebecca; Sawyer, Cheryl; Sloan, Eva D.

    2015-01-01

    This collective case study investigated the experiences of bilingual counselors-in-training who assessed school-wide cultural competence in public schools. Analysis and interpretation of data resulted in the identification of 5 themes: eye-opening experiences, recognition of strengths, the role of school leaders, road maps for change, and…

  18. Cultural Competence and School Counselor Training: A Collective Case Study

    Science.gov (United States)

    Nelson, Judith A.; Bustamante, Rebecca; Sawyer, Cheryl; Sloan, Eva D.

    2015-01-01

    This collective case study investigated the experiences of bilingual counselors-in-training who assessed school-wide cultural competence in public schools. Analysis and interpretation of data resulted in the identification of 5 themes: eye-opening experiences, recognition of strengths, the role of school leaders, road maps for change, and…

  19. Cultures of the World: Administrative Manual [And] Study Guide.

    Science.gov (United States)

    Richardson, Charles Howard

    This learning package is a three-semester-hour, independent-study course in geography and cultures of the world designed for postsecondary, external degree students. Keyed to the commercially published textbook "Between Two Worlds: A New Introduction to Geography" (Boston, MA: Houghton Mifflin Company, 1973), the package consists of an…

  20. Transforming Cultures of Care: A Case Study in Organizational Change

    Science.gov (United States)

    Purvis, Karyn; Cross, David; Jones, Daren; Buff, Gary

    2012-01-01

    The authors report on a small organizational case study highlighting the dimensions of trauma-informed care, the processes of organizational change, and the growth of caregiver expertise. The article is framed by the notion of caregiving cultures, which refers to the beliefs, languages, and practices of caregivers and caregiving organizations.…

  1. Study Abroad: Enhanced Learning Experience in Cultural Diversity

    Science.gov (United States)

    Jaoko, Japheth

    2010-01-01

    This paper examines how a study abroad experiential learning course in diversity provided a cultural immersion experience for a group of social work students from a small private university in central Kentucky. The students participated in a three-week international education experience in Kenya and reported this experience helped them become more…

  2. A Cross-cultural Study on American and Chinese

    Institute of Scientific and Technical Information of China (English)

    李志远

    2015-01-01

    Compliment is one of the most commonly used speech acts in social communication. This thesis, through the comparative studies on topic distribution and compliment response of Chinese compliments and Ameri-can compliments, aims at helping English learners have a profound understanding on compliments in cross-cultural communication.

  3. Studying Another Culture through Children's Games: Examples from Nigeria.

    Science.gov (United States)

    Sunal, Cynthia Szymanski

    1988-01-01

    Identifying games as being common to children worldwide, Sunal focuses on Nigerian group games in order to illustrate the range of game variations and their use in a social studies program. Describes hiding games, singing games, and a fine-motor skills game, stating that they help acquaint students with foreign cultures and reveal similarities in…

  4. Transforming Cultures of Care: A Case Study in Organizational Change

    Science.gov (United States)

    Purvis, Karyn; Cross, David; Jones, Daren; Buff, Gary

    2012-01-01

    The authors report on a small organizational case study highlighting the dimensions of trauma-informed care, the processes of organizational change, and the growth of caregiver expertise. The article is framed by the notion of caregiving cultures, which refers to the beliefs, languages, and practices of caregivers and caregiving organizations.…

  5. Marxism in Vygotskian Approaches to Cultural Studies of Science Education

    Science.gov (United States)

    Lima, Paulo, Jr.; Ostermann, Fernanda; Rezende, Flavia

    2014-01-01

    In this paper we initially address the main categories of Marxism, illustrating how Vygotsky has appropriated them as mediational meta-theoretical tools for building concepts for his psychological approach. In order to investigate the influence of Marxism in cultural studies of science education, we make an account of how current research,…

  6. Armand Mattelard et Erik Neveu. Introduction aux Cultural Studies.

    Directory of Open Access Journals (Sweden)

    Michel Imbert

    2006-04-01

    Full Text Available Cet ouvrage retrace en cinq chapitres la génèse et le développement d’un courant de recherche jusqu’à un certain point méconnu en France. Dans les années 60, l’école de Birmingham (Centre for Contemporary Cultural Studies a élargi la notion de culture au sens noble du terme en accordant aux styles de vie de la classe ouvrière et aux médias l’attention jusque là réservée à la culture des lettrés.Le premier chapitre évoque les travaux des pères fondateurs : le retentissement profond de l’ouvra...

  7. Culture, perception, and artistic visualization: a comparative study of children's drawings in three Siberian cultural groups.

    Science.gov (United States)

    Istomin, Kirill V; Panáková, Jaroslava; Heady, Patrick

    2014-01-01

    In a study of three indigenous and non-indigenous cultural groups in northwestern and northeastern Siberia, framed line tests and a landscape drawing task were used to examine the hypotheses that test-based assessments of context sensitivity and independence are correlated with the amount of contextual information contained in drawings, and with the order in which the focal and background objects are drawn. The results supported these hypotheses, and inspection of the regression relationships suggested that the intergroup variations in test performance were likely to result from differences in the attention accorded to contextual information, as revealed by the drawings. Social and environmental explanations for the group differences in context sensitivity are also discussed. The conclusions support the argument that cultural differences in artistic styles and perceptual tests reflect the same underlying perceptual tendencies, and they are consistent with the argument that these tendencies reflect corresponding differences in patterns of social and environmental interaction.

  8. Preparation and Characterization of Pure Rutile TiO2 Nanoparticles for Photocatalytic Study and Thin Films for Dye-Sensitized Solar Cells

    Directory of Open Access Journals (Sweden)

    Huei-Siou Chen

    2011-01-01

    Full Text Available Pure rutile-phase TiO2 (r-TiO2 was synthesized by a simple one pot experiment under hydrothermal condition using titanium (IV n-butoxide as a Ti-precursor and HCl as a peptizer. The TiO2 products were characterized by XRD, TEM, ESCA, and BET surface area measurement. The r-TiO2 were rodlike in shape with average size of ∼61×32 nm at hydrothermal temperature of 220°C for 10 h. Hydrothermal treatment at longer reaction time increased the tendency of crystal growth and also decreased the BET surface area. The degradation of methylene blue was selected as a test reaction to confer the photocatalytic activity of as-obtained r-TiO2. The results showed a strong correlation between the structure evolution, particle size, and photocatalytic performance of r-TiO2. Furthermore, the r-TiO2-based solar cell was prepared for the photovoltaic characteristics study, and the best efficiency of ~3.16% was obtained.

  9. B3LYP periodic study of the physicochemical properties of the nonpolar (010) Mg-pure and fe-containing olivine surfaces.

    Science.gov (United States)

    Navarro-Ruiz, Javier; Ugliengo, Piero; Rimola, Albert; Sodupe, Mariona

    2014-08-07

    B3LYP periodic simulations have been carried out to study some physicochemical properties of the bulk structures and the corresponding nonpolar (010) surfaces of Mg-pure and Fe-containing olivine systems; i.e., Mg2SiO4 (Fo) and Mg1.5Fe0.5SiO4 (Fo75). A detailed structural analysis of the (010) Fo and Fo75 surface models shows the presence of coordinatively unsaturated metal cations (Mg(2+) and Fe(2+), respectively) with shorter metal-O distances compared to the bulk ones. Energetic analysis devoted to the Fe(2+) electronic spin configuration and to the ion position in the surfaces reveals that Fe(2+) in its quintet state and placed at the outermost positions of the slab constitutes the most stable Fe-containing surface, which is related to the higher stability of high spin states when Fe(2+) is coordinatively unsaturated. Comparison of the simulated IR and the corresponding reflectance spectra indicates that Fe(2+) substitution induces an overall bathochromic shift of the spectra due to the larger mass of Fe compared to Mg cation. In contrast, the IR spectra of the surfaces are shifted to upper values and exhibit more bands compared to the corresponding bulk systems due to the shorter metal-O distances given in the coordinatively unsaturated metals and to symmetry reduction which brings nonequivalent motions between the outermost and the internal modes, respectively.

  10. Exploring classical conditioning for strengthening the links between semantic and lexical representations in pure anomia: Preliminary findings from a single case study.

    Science.gov (United States)

    Zannino, Gian Daniele; Barban, Francesco; Caltagirone, Carlo; Carlesimo, Giovanni A

    2017-03-01

    The same language symptom might arise at different functional loci in people with aphasia. Therefore, it is plausible that different therapeutic interventions should be adopted to approach the same difficulties in different patients. Although this point of view is still widely accepted, recently the focus has shifted from the functional locus of a rehabilitative intervention to the mechanisms of action underlying the relearning process. We maintain that both aspects should be taken into account when programming a rehabilitative intervention; furthermore, investigating relearning mechanisms might shed new light on the functional architecture of the disrupted processes. Here, we investigated, in a single case study, whether classical conditioning was a suitable relearning paradigm for targeting word-finding difficulties in pure anomia, that is in a patient with an impairment in accessing intact output lexical representations from a spared semantic system. Using a word-repetition task on picture presentation, we contrasted a condition in which the stimulus onset asynchrony between word and picture stimuli was well suited to produce classical conditioning with a condition in which repetition training could not benefit from this learning mechanism. Only classical conditioning training exerted a significant, long-lasting effect on our patient's naming skill. Tentative implications of our results for the functional architecture of single-word processing are discussed. © 2015 The British Psychological Society.

  11. 纯弯状态下蜂窝梁腹板稳定问题研究%STUDY ON WEB STABILITY OF CASTELLATED BEAMS UNDER PURE BENDING CONDITION

    Institute of Scientific and Technical Information of China (English)

    王庆利; 曹平周; 徐成章; 蒋力

    2001-01-01

    针对国内尚无有关蜂窝构件的规范或标准可以执行的实际情况,进行了蜂窝梁腹板在纯弯状态下的稳定问题研究.基于薄板的弹性稳定理论,建立了孔口处腹板和孔间处度板的计算模型;应用能量法,得出了板的稳定方程,确定了板的高厚比限值和最小弯曲刚度,可供有关设计参考.%As there is no criterion or standard about castellated member athome, study of the web stability of castellated be ams under pure bending condition is carried out. Based on elastic stability theory of thin plate, the calculation model of web between opening and web above opening of castellated beams are formulated Using energy method, the stability equations are deduced, and the depth-thickness ratio and minimun bending rigidity are decided These can be consulted by designs.

  12. Fabrication of pure and Ag-doped TiO2 nanorods and study of the lattice strain and the activation energy of the crystalline phases

    Science.gov (United States)

    Riazian, Mehran; Rad, Shima Daliri; Azinabadi, Reza Ramezani

    2013-02-01

    TiO2 nanorods can be used as dye-sensitized solar cells and as various sensors and photocatalysts. These nanorods are synthesized by using a thermal corrosion process in a NaOH solution at 200 °C with TiO2 powder as a source material. In the present work, the synthesis of TiO2 nanorods in anatase, rutile and Ti8O15 phases and the synthesis of TiO2 nanorods by using the sol-gel method and alkaline corrosion to incorporate silver and silver-oxide dopants are reported. The morphologies and the crystalline structures of the TiO2 nanorods are characterized using field emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM), tunneling electron microscopy (TEM) and X-ray diffraction (XRD) techniques. The obtained results show an aggregation structure at high calcining temperatures with spherical particles and with Ti-O-Ti, Ti-O and Ag-O bonds. The effects of the chemical composition and the calcining temperature on the surface topography, lattice strain and phase crystallization are studied. The activation energy (E) of nanoparticle formation in a pure state during thermal treatment is calculated.

  13. Education for a Culture of Peace: The Culture of Peace News Network as a Case Study

    Science.gov (United States)

    Adams, David

    2013-01-01

    The Culture of Peace News Network, an internet news service, is analyzed in the framework of a general approach to education for a culture of peace. Its format reflects the eight program areas for a culture of peace as adopted by the UN General Assembly. Among its other operating principles are universality of news with all cultures and regions of…

  14. Visual processing in pure alexia

    DEFF Research Database (Denmark)

    Starrfelt, Randi; Habekost, Thomas; Gerlach, Christian

    2010-01-01

    Whether pure alexia is a selective disorder that affects reading only, or if it reflects a more general visual disturbance, is highly debated. We have investigated the selectivity of visual deficits in a pure alexic patient (NN) using a combination of psychophysical measures, mathematical modelling...

  15. A conceptual approach to studying the organisational culture of construction projects

    Directory of Open Access Journals (Sweden)

    Thayaparan Gajendran

    2012-05-01

    Full Text Available Studying the culture of project organisations requires a robust theoretical framework, which provides a platform for generating understanding. It is argued that cultural analysis frameworks are most effectively conceptualised from multiple philosophical and multi-methodological positions. To this end this paper presents a cultural analysis framework for studying construction project organisations, based on a synthesis of the culture literature. Four key aspects underpinning organisational cultural framework are explored: the paradigms used to conceptualise organisational culture, the methods by which individuals represent and assess cultural dimensions, the cultural perspectives assumed by the observer when defining and describing culture, and the managers’ orientation to culture in their organisations. The proposed framework comprises three synthesised cultural philosophical positions: integration-technical, differentiation-practical and fragmentation-emancipation. These philosophical positions span the polar extremes defining the cultural paradigm continuum, which together provide researchers and organisational managers with a sound foundation from which to study the culture of project organisations.

  16. A conceptual approach to studying the organisational culture of construction projects

    Directory of Open Access Journals (Sweden)

    Thayaparan Gajendran

    2012-05-01

    Full Text Available Studying the culture of project organisations requires a robust theoretical framework, which provides a platform for generating understanding. It is argued that cultural analysis frameworks are most effectively conceptualised from multiple philosophical and multi-methodological positions. To this end this paper presents a cultural analysis framework for studying construction project organisations, based on a synthesis of the culture literature. Four key aspects underpinning organisational cultural framework are explored: the paradigms used to conceptualise organisational culture, the methods by which individuals represent and assess cultural dimensions, the cultural perspectives assumed by the observer when defining and describing culture, and the managers’ orientation to culture in their organisations. The proposed framework comprises three synthesised cultural philosophical positions: integration-technical, differentiation-practical and fragmentation-emancipation. These philosophical positions span the polar extremes defining the cultural paradigm continuum, which together provide researchers and organisational managers with a sound foundation from which to study the culture of project organisations.  

  17. Study on and Redefinition of the Concept of Culture

    Institute of Scientific and Technical Information of China (English)

    戴剑飞

    2015-01-01

    So far, various opinions about the concept of culture are come up with in both China and the West in which three kinds of culture are classified as follows. The culture in narrow sense denotes merely the ideas. The culture in medium sense embraces ideas, customs, norms, symbols and behaviors, etc. The culture in broad sense refers to the lifestyle constituted of both the material culture and spiritual culture. There is an urgent necessity to redefine the concept of culture by exploring the history of the etymolo-gy of the word"culture"in order to figure out the deviance of different concepts of culture.

  18. cultural

    Directory of Open Access Journals (Sweden)

    Irene Kreutz

    2006-01-01

    Full Text Available Es un estudio cualitativo que adoptó como referencial teorico-motodológico la antropología y la etnografía. Presenta las experiencias vivenciadas por mujeres de una comunidad en el proceso salud-enfermedad, con el objetivo de comprender los determinantes sócio-culturales e históricos de las prácticas de prevención y tratamiento adoptados por el grupo cultural por medio de la entrevista semi-estructurada. Los temas que emergieron fueron: la relación entre la alimentación y lo proceso salud-enfermedad, las relaciones con el sistema de salud oficial y el proceso salud-enfermedad y lo sobrenatural. Los dados revelaron que los moradores de la comunidad investigada tienen un modo particular de explicar sus procedimientos terapéuticos. Consideramos que es papel de los profesionales de la salud en sus prácticas, la adopción de abordajes o enfoques que consideren al individuo en su dimensión sócio-cultural e histórica, considerando la enorme diversidad cultural en nuestro país.

  19. Sex Dilemmas, Amazons and Cyborgs: Feminist Cultural Studies and Sport

    Directory of Open Access Journals (Sweden)

    Helena Tolvhed

    2013-06-01

    Full Text Available In this article, I discuss sport and physical activities as a field of empirical investi-gation for feminist cultural studies with a potential to contribute to theorizing the body, gender and difference. Sport has, historically, served to legitimize and rein-force the gender dichotomy by making men “masculine” through developing phys-ical strength and endurance, while women generally have been excluded or di-rected towards activities fostering a “feminine suppleness”. The recent case of runner Caster Semenya, who was subjected to extensive gender tests, demon-strates how athletic superiority and “masculine” attributes in women still today stir public emotions and evoke cultural anxieties of gender blurring. But the rigid gen-der boundaries have also made sport a field of transgressions. From the “Soviet amazon” of the Cold War, transgressions in sport have publicly demonstrated, but also pushed, the boundaries of cultural understandings of gender. Gender verifica-tion tests have exposed a continuum of bodies that cannot easily be arranged into two stable, separate gender categories.In spite of the so called “corporeal turn”, sport is still rather neglected within cultural studies and feminist research. This appears to be linked to a degradation, and fear, of the body and of the risk that women – once again – be reduced to biology and physical capacity. But studies of sport might further develop under-standings of the processes through which embodied knowledge and subjectivity is produced, in a way that overcomes the split between corporeality and discursive regimes or representations. Furthermore, with the fitness upsurge since the 1980s, the athletic female body has emerged as a cultural ideal and a rare validation of “female masculinity” (Halberstam in popular culture. This is an area well-suited for “third wave” feminist cultural studies that are at ease with complexities and contradictions: the practices and

  20. 镓含量对纯铝的耐蚀性的影响%Studies on the Influence of Gallium Content on the Corrosion Resistance of Pure Aluminum

    Institute of Scientific and Technical Information of China (English)

    LIN Jin-ping; WANG Jun; SUN Bao-de

    2008-01-01

    The effect of small amount gallium (up to 0.06%) on the corrosion behavior of pure aluminum (99.99%,4N) in chloride solution was investigated using a potentiodynamic polarization technique.It has been found that the open circuit corrosion potential and the pitting potential shifted in the active (negative) direction with increasing gallium content,while corrosion rate and pitting occurrence factor for aluminum increased.The effect of gallium on the degradation of corrosion resistance is rather small while the gallium content is below 0.03mass%.The gallium content should be kept less than 0.03mass% in the pure aluminum.

  1. Using Game Theory to Study the Evolution of Cultural Norms

    CERN Document Server

    De, Soham; Gelfand, Michele

    2016-01-01

    We discuss how to use evolutionary game theory (EGT) as a framework for studying how cultural dynamics and structural properties can influence the evolution of norms and behaviors within a society. We provide a brief tutorial on how EGT works, and discuss what kinds of insights it can provide. We then describe three published studies in which we have developed EGT models that help explain how structural and external conditions in a society affect the emergence of social norms.

  2. Les Problemes de l'alexie pure (Problems of Pure Alexia)

    Science.gov (United States)

    Kremin, Helgard

    1976-01-01

    This article reviews studies done on alexia and describes experiments designed to distinguish qualitatively between pure alexia (marked by the absence of oral and written problems) and other forms of alexia. (Text is in French.) (CLK)

  3. Microbiology of shallow subsurface aquifer and carbonate rocks studied by culture-dependent and culture-independent methods. Preliminary results on an underground laboratory, the LSBB, Rustrel, France

    Directory of Open Access Journals (Sweden)

    Galès Grégoire

    2016-01-01

    Full Text Available We investigated the microbiology of a shallow subsurface site, the LSBB, located near Avignon. This site lies in carbonate rocks, belonging to the Urgonian facies. Rock, concrete and water samples were collected and directly transferred to the laboratory. Studies of microorganisms as pure cultures are the only way to get their real physiological properties. Nevertheless, microbiologists cannot cultivate and isolate the majority of microorganisms for several reasons, one being our lack of understanding of their minimal needs. Molecular studies, e.g. extraction and sequencing of the total nucleic acids present in an environment provide phylogenetic and metabolic information on uncultivated microorganisms. We performed aerobic and anaerobic culture with various electron acceptors and donors, searching for heterotrophic, methanogenic, sulphate- nitrate- and FeIII- reducing Prokaryotes. We also performed DNA extractions and PCR amplification of ribosomal RNA genes, to test if our protocols were adapted to this environment. Our results show that the LSBB galleries are colonized by a low diversity microbiote, with a strong influence of anthropogenic activities. Further studies will link the microorganisms biodiversity and the petrophysic properties of rocks.

  4. Study of the Relationship between Cultural differences and Language teaching

    Institute of Scientific and Technical Information of China (English)

    孟庆瑜

    2014-01-01

    Language is an important part of culture,each language belong to a certain culture.Language and culture are interdependent from each other.So,language teaching must be concerned with teaching the culture which it belongs to.Language teaching should pay more attention to the cultural differences.

  5. A Study of the Cultural Factors Influencing SLA

    Institute of Scientific and Technical Information of China (English)

    LiTong; HuangRenfeng; YangWeizhong

    2004-01-01

    The cultural factors that affect Second Language Acquisition (SLA) result from many aspects, some derived from the level of the acquirer's mother language, other soriginated from the stratum of his native culture. Based on the relations between language and culture - language is the carrier of culture, while culture is the contents of language-it

  6. Promoting and Financing Cultural Tourism in Europe through European Capitals of Culture: A Case Study of Košice, European Capital of Culture 2013

    Directory of Open Access Journals (Sweden)

    Miriam Šebová

    2014-05-01

    Full Text Available The paper highlights the link between the European Capital of Culture (ECoC designation and the development of cultural tourism in Europe. Cultural tourism is the fastest growing segment of tourism in the world and is the desirable objective of new defined cultural policies in European cities. According to the European Commission (2012, it is estimated that cultural tourism accounts for around 40% of all European tourism. In general, the European Commission confirms the significant role of cultural tourism in the development of tourism in Europe and one of the most visible tools of this strategy is the designation of the ECoC. Cultural policy has also become an important tool of urban regeneration and in the rebranding of European cities. The case study of Košice ECoC 2013 presents part of the results from the Košice ECoC evaluation project. The Košice ECoC 2013 project was designed as a community led project which was targeted at increasing the cultural consumption of residents rather than the number of foreign cultural tourists. However, the biggest events such as White night attracted a lot of tourists from the wider region. More than 70 million EUR was dedicated to investments, which have significantly improved the culture infrastructure in Košice. The ECoC designation supported the local cultural buzz and led to new local production of modern culture, art and the creative industries. Other visible effects have already been seen in the advantages gained by the decentralization of the cultural infrastructure and events in the neighbourhoods. The involvement of people outside the city centre has avoided gentrification in the city. From this point of view the project Košice ECoC 2013 has had the prerequisite to sustainable cultural tourism in Košice

  7. Experimental study of bioartificial liver with cultured human liver cells

    Institute of Scientific and Technical Information of China (English)

    1999-01-01

    AIM To establish an extracorporeal bioartificial liver support system (EBLSS) using cultured human liver cells and to study its support effect for fulminant hepatic failure (FHF).METHODS The liver support experiment of EBLSS consisting of aggregates cultured human liver cells, hollow fiber bioreactor, and circulation unit was carried out in dizhepatic dogs.RESULTS The viability of isolated hepatocytes and nonparenchymal liver cells reached 96%. These cells were successfully cultured as multicellular spheroids with synthetic technique. The typical morphological appearance was retained up to the end of the artificial liver experiment. Compared with the control dogs treated with EBLSS without liver cells, the survival time of artificial liver support dogs was significantly prolonged. The changes of blood pressure, heart rate and ECG were slow. Both serum ammonia and lactate levels were significantly lowered at the 3rd h and 5th h. In addition, a good viability of human liver cells was noted after 5 h experiment.CONCLUSION EBLSS playing a metabolic role of cultured human hepatocytes, is capable of compensating the function of the liver, and could provide effective artificial liver support and therapy for patients with FHF.

  8. [Cytotoxicity studies on T-3262 in cultured Chinese hamster cells].

    Science.gov (United States)

    Yoneda, T; Nakamura, S; Nojima, Y; Nishio, Y

    1989-04-01

    T-3262 is an antibacterial drug which belongs to the group of pyridonecarboxylic acids. In this study, we investigated cytotoxicity of T-3262 for inhibition of cell growth and effects on viability of, and morphological changes in cultured Chinese hamster cells (V79 cells). The following results were obtained. 1. The 50% inhibition dose of T-3262 for cell growth (ID50, cultured for 48 hours) was 12 micrograms/ml, showing that the inhibitory effect of T-3262 on the cell growth was stronger than that of enoxacin (ENX: ID50 44 micrograms/ml), norfloxacin (NFLX: ID50 105 micrograms/ml) or ofloxacin (OFLX: ID50 145 micrograms/ml). 2. The number of cells increased and dead cells were scarcely seen at the highest concentration tested in culture medium (40 micrograms/ml of T-3262 for 48 hours). At this concentration, degeneration of cytoplasm (atrophy and round shape) and decrease of mitotic cells were observed. These morphological changes were similar to those of the cells treated 400 micrograms/ml of NFLX or OFLX for 48 hours. 3. After the removal of T-3262 from culture medium, the cells began to grow actively and recovered from the morphological changes. The similar phenomenon was observed with ENX treated cells but not with fluorouracil or mitomycin C treated cells.

  9. An Exploratory Study of the Drivers of Student Satisfaction and Learning Experience in Hybrid-Online and Purely Online Marketing Courses

    Science.gov (United States)

    Estelami, Hooman

    2012-01-01

    Much of the existing research in distance education has focused on contrasting the outcomes between traditional face-to-face teaching and purely online courses, in which the entire course content is delivered online. However, research has not examined the effectiveness of hybrid-online courses, in which a combination of online delivery and…

  10. Interaction of human plasma fibrinogen with commercially pure titanium as studied with atomic force microscopy and X-ray photoelectron spectroscopy.

    Science.gov (United States)

    Keere, Isabel Van De; Willaert, Ronnie; Hubin, Annick; Vereecken, Jean

    2008-03-04

    The surface of a biomaterial interacts with the body fluid upon implantation in the human body. The biocompatibility of a material is strongly influenced by the adsorption of proteins onto the surface. Titanium is frequently used as a biomaterial for implants in orthopedics and cardiovascular devices. Understanding the biocompatibility is very important to improve implants. The surface chemistry of an implant material and its influence on the interaction with body fluid is crucial in that perspective. The main goal of this study was to investigate the conformation of human plasma fibrinogen (HPF) adsorbed on commercially pure titanium (CP Ti) on a molecular level by means of ex situ atomic force microscopy (AFM). With X-ray photoelectron spectroscopy combined with argon ion beam depth profiling, it was shown that the oxide layer present at the surface was mainly composed of TiO2, with a small percentage of Ti2O3. Ex situ AFM imaging showed the conformation of HPF on CP Ti. Single molecules and aggregates of fibrinogen were observed. The trinodular structure of single HPF molecules (two spherical D domains at the distal ends of the extended molecule and the central spherical E domain) adsorbed onto CP Ti was visualized. Aggregate formation through the connection of the D domains of the HPF molecules was observed on CP Ti. The alphaC domains of HPF were not visible on CP Ti. The ex situ AFM images indicated conformational changes of HPF upon adsorption onto CP Ti. The conformation of the adsorbed HPF molecules was different on mica and titanium. The difference in wettability between both substrates caused a larger spread of the protein on the CP Ti surface and thus resulted in a larger perturbation to the native structure of HPF as compared to mica.

  11. Comparison of hydrogen storage properties of pure Mg and milled pure Mg

    Indian Academy of Sciences (India)

    Myoung Youp Song; Young Jun Kwak; Seong Ho Lee; Hye Ryoung Park

    2014-06-01

    Hydrogen storage properties of pure Mg were studied at 593 K under 12 bar H2. In order to increase the hydriding and dehydriding rates, pure Mg was ground under hydrogen atmosphere (reactive mechanical grinding, RMG) and its hydrogen storage properties were subsequently investigated. Pure Mg absorbed hydrogen very slowly. At the number of cycles () of 1, pure Mg absorbed 0.05 wt% H for 5 min, 0.08 wt% H for 10 min and 0.29 wt% H for 60 min at 593 K under 12 bar H2. Activation was completed at the fifth cycle. At = 6, pure Mg absorbed 1.76 wt% H for 5 min, 2.17 wt% H for 10 min and 3.40 wt% H for 60 min. The activation of pure Mg after RMG was completed at the sixth cycle. At = 7, pure Mg after RMG absorbed 2.57 wt% H for 5 min, 3.21 wt% H for 10 min and 4.15 wt% H for 60 min.

  12. Identifying sports cultural opportunity – Case Study of Mazandaran Province

    Directory of Open Access Journals (Sweden)

    Ahmad Ahmadzadeh

    2013-09-01

    Full Text Available Sport is an important tool to draw the national attention. Sport is a big assembly to make identity. Also, sport is a cause for identity and unity. The purpose of this study is to identify the cultural opportunity of sport in the province of Mazandaran in Iran. The research method was mixed. Sequential exploratory mixed method of the first method is qualitative and quantitative methods. The study included all the Mazandaran managers, coaches, experts, judges and athletes at the national level. The results show that directors, judges and experts believe that people's happiness and euphoria in winning the sports events is the most sport important cultural opportunity in Mazandaran (All kinds of sport were included. A lot of money is spent on TV programs and movies to fill the people's free time.

  13. Cultural Studies and the Subaltern: Theory and Practice

    Directory of Open Access Journals (Sweden)

    Ágnes Györke

    2012-05-01

    Full Text Available My article discusses the theoretical and practical implications of applying the methodology of cultural studies, as it is delineated by Stuart Hall, in the East-Central European context. Despite the celebrated “internationalization” of the discipline as well as “de-Eurocentrizing” initiatives, a number of scholars, such as G. C. Spivak and Hall himself, claim that research taking a cultural studies approach has offered little innovative intervention in recent years, and the discipline remains defined by a Western, (postmodern theoretical framework. I argue that scholars in Hungary (and Slovenia have an unprecedented opportunity to contribute to the field, yet in order to avoid falling into the trap of repeating obvious claims and conclusion, we need to take an approach that Spivak associates with the toleration of uncertainty and paradox, and Jessica Benjamin calls intersubjective interaction.

  14. The Study on the Extraction Technology for Pure Mitochondrial DNA from Sesame%芝麻高纯度线粒体DNA提取技术研究

    Institute of Scientific and Technical Information of China (English)

    黎冬华; 王林海; 张艳欣; 吕海霞; 张晓燕; 刘佳; 张秀荣

    2011-01-01

    Using etiolated seedlings of 2 sesame varieties, an economic, rapid, efficient sesame seed mitochondria DNA ( mtDNA) extraction method was established through the key factor Dnase digestion time, including homogenizing processing, differential eentrifugation comprising 2 400 g centrifugation for 15 min and 12 000 g centrif-ugation for 12 min to acquire primary mitochondria, Dnase digestion for 1.5 h to eliminate the pollution of genome DNA, centrifugation with 0.6 mol/L sucrose padding to get pure mitochondria, and SDS-proteinase K lysis to obtain pure mtDNA. The mtDNA samples were tested using DNA/Protein Analyzer, PCR with genome-special markers ( nuclear gene-specific primer beta-actin, mitochondrial gene-specific primer rrn26S and chloroplast gene-specific primer ARCP5 ) and 1.0% agarose gel electrophoresis. It was proved that the mtDNA isolated by this method not only has the yield more than 1 μg per gram material, but also characterized of high purity. The results showed that this high quality mtDNA can be successfully used in genetic studies as sesame cytoplasmic male sterility and stress resistance.%以2个芝麻品种黄化苗为试验材料,通过比较植物线粒体DNA(mtDNA)提取过程中的关键影响因素DNase处理时间,建立了一种经济、快速、高效的芝麻mtDNA提取方法.该方法分别通过匀浆化处理、2 400 g离心15min结合12 000 g离心12 min的差速离心方法分离获得粗制线粒体,再经过DNase酶切处理1.5 h去除基因组DNA的污染,然后经0.6 m0l/L蔗糖衬垫离心获得纯的线粒体,通过SDS-蛋白酶K方法裂解线粒体获得芝麻mtDNA.经过核酸蛋白分析仪分析表明:本技术每1 g芝麻黄化苗能获得1 P,g以上mtDNA;通过1.0%琼脂糖凝胶电泳检测表明获得mtDNA完整,无明显降解;并利用三类特异引物(核基因特异引物beta-actin、线粒体基因特异引物rrn26S以及叶绿体基因特异引物ARCP5)进行PCR检测表明提取的mtDNA为高纯度.芝麻

  15. Measuring innovation culture: Application at four case studies

    Directory of Open Access Journals (Sweden)

    Manuel Ramón Tejeiro Koller

    2014-05-01

    Full Text Available Purpose: The present article proposes a tool for the measurement of a corporate culture that makes companies more innovative. In a fast changing environment, such as the current one, the capacity to innovate has become very important. It allows companies to adapt better and faster or even leapfrog competition by creating the change themselves. Organizational Theory has come to understand corporate culture –a group of shared values and philosophies– as a powerful management tool and it is due to this that me intent to measure it. Design/methodology: After extracting the characteristics of an innovation culture from the literature review, a group of indicators relating to these cultural traits was selected. The further revision of prior measurement tools allowed us to compare the findings and summarize our selection in a set of 67 questions. In order to contrast he validity of these, we applied the questionnaire at four innovative firms. During this process we tried to confirm our indicators and add new ones, resulting from the conducted interviews, introducing an inductive perspective to our study. Findings: We conclude with a revised and expanded version of the questionnaire, which includes a revision of the indicators and the contributions of the interviewees. Through this process we believe to have contributed to elaborating a measurement tool for innovation culture, since we were able to apply the majority of questions selected in the first place, eliminating and redefining the others. A revised version of the questionnaire is offered for further research. Practical implications: The measurement tool developed in this article allows those managers interested in benchmarking their company’s corporate culture against one that promotes innovation. We increased the capability of expressing one of the most significant intangibles of the company, thus increasing its visibility and value recognition. Originality/value: The contributes in the

  16. Studies of In Vitro Embryo Culture of Guppy (Poecilia reticulata)

    OpenAIRE

    Liu, Lili; Lee, Ki-Young

    2014-01-01

    Different with other fishes, the guppies (Poecilia reticulata) is ovoviviparity, which retain their fertilized eggs within the follicle throughout gestation. The synchronously growing diplotene oocytes store nutrients in droplets and yolk, before their maturation and fertilization. The lecithotrophic strategy of development entails the provisioning of embryos with resources from the maternal yolk deposit rather than from a placenta, it allows the extracorporeal culture of guppy embryo. Studie...

  17. Implicit Reading in Chinese Pure Alexia

    Science.gov (United States)

    Shan, Chunlei; Zhu, Renjing; Xu, Mingwei; Luo, Benyan; Weng, Xuchu

    2010-01-01

    A number of recent studies have shown that some patients with pure alexia display evidence of implicit access to lexical and semantic information about words that they cannot read explicitly. This phenomenon has not been investigated systematically in Chinese patients. We report here a case study of a Chinese patient who met the criteria for pure…

  18. Cultural Phenomena Seen from the Perspective of Language Issues (Globalisation, Multiculturalism, Interculturalism and Transculturalism): Preliminary Reflections for Cultural Studies

    OpenAIRE

    Tendera, Paulina

    2016-01-01

    In the wake of developing methods of cultural research, the language of cultural studies is undergoing evolution and enrichment as well. This phenomenon obviously applies to all evolving academic fields, but in the area of the humanities a particular impact, consisting of the (sometimes deliberate) creation of successive cultural spaces, is manifested. For example, the introduction of a new concept may result in the initiation of a trend in the description and categorisation of reality. This ...

  19. Cultural and Personality Predictors of Facebook Intrusion: A Cross-Cultural Study

    Science.gov (United States)

    Błachnio, Agata; Przepiorka, Aneta; Benvenuti, Martina; Cannata, Davide; Ciobanu, Adela M.; Senol-Durak, Emre; Durak, Mithat; Giannakos, Michail N.; Mazzoni, Elvis; Pappas, Ilias O.; Popa, Camelia; Seidman, Gwendolyn; Yu, Shu; Wu, Anise M. S.; Ben-Ezra, Menachem

    2016-01-01

    The increase in the number of users of social networking sites (SNS) has inspired intense efforts to determine intercultural differences between them. The main aim of the study was to investigate the cultural and personal predictors of Facebook intrusion. A total of 2628 Facebook users from eight countries took part in the study. The Facebook Intrusion Questionnaire, the Ten-Item Personality Inventory, and the Singelis Scale were used. We found that two variables related to Country were significantly related to Facebook intrusion: uniqueness (negatively) and low context (positively); of the personality variables, conscientiousness, and emotional stability were negatively related to the dependent variable of Facebook intrusion across different countries, which may indicate the universal pattern of Facebook intrusion. The results of the study will contribute to the international debate on the phenomenon of SNS. PMID:27994566

  20. Cultural and Personality Predictors of Facebook Intrusion: A Cross-Cultural Study

    Directory of Open Access Journals (Sweden)

    Agata Błachnio

    2016-12-01

    Full Text Available The increase in the number of users of social networking sites has inspired intense efforts to determine intercultural differences between them. The main aim of the study was to investigate the cultural and personal predictors of Facebook intrusion. A total of 2,628 Facebook users from eight countries took part in the study. The Facebook Intrusion Questionnaire, the Ten-Item Personality Measure, and the Singelis Scale were used. We found that two variables related to Country were significantly related to Facebook intrusion: uniqueness (negatively and low context (positively; of the personality variables, conscientiousness and emotional stability were negatively related to the dependent variable of Facebook intrusion across different countries, which may indicate the universal pattern of Facebook intrusion. The results of the study will contribute to the international debate on the phenomenon of social networking sites (SNS.

  1. Cultural and Personality Predictors of Facebook Intrusion: A Cross-Cultural Study.

    Science.gov (United States)

    Błachnio, Agata; Przepiorka, Aneta; Benvenuti, Martina; Cannata, Davide; Ciobanu, Adela M; Senol-Durak, Emre; Durak, Mithat; Giannakos, Michail N; Mazzoni, Elvis; Pappas, Ilias O; Popa, Camelia; Seidman, Gwendolyn; Yu, Shu; Wu, Anise M S; Ben-Ezra, Menachem

    2016-01-01

    The increase in the number of users of social networking sites (SNS) has inspired intense efforts to determine intercultural differences between them. The main aim of the study was to investigate the cultural and personal predictors of Facebook intrusion. A total of 2628 Facebook users from eight countries took part in the study. The Facebook Intrusion Questionnaire, the Ten-Item Personality Inventory, and the Singelis Scale were used. We found that two variables related to Country were significantly related to Facebook intrusion: uniqueness (negatively) and low context (positively); of the personality variables, conscientiousness, and emotional stability were negatively related to the dependent variable of Facebook intrusion across different countries, which may indicate the universal pattern of Facebook intrusion. The results of the study will contribute to the international debate on the phenomenon of SNS.

  2. Data management in a longitudinal cross-cultural study.

    Science.gov (United States)

    Roberts, R J; Musick, B S; Olley, B; Hall, K S; Hendrie, H C; Oyediran, A B

    The Indianapolis-Ibadan Dementia Project compares the rates of dementia at two sites, one in the U.S.A. and one in Nigeria. This paper concentrates on the data management issues in this longitudinal cross-cultural study. Approximately 2500 elderly people were recruited at each site, and continue to be re-assessed every two years. All the data are collected on paper and then entered into a FoxPro relational database. Most of the data management, including data cleaning, is done in Indianapolis. The design of the data collection forms is particularly important in a cross-cultural study, with the questions and the coding of responses clear and simple. Since Nigeria and the U.S.A. have different levels of technological development, the computer hardware and software were chosen to be suitable for use at either site. Exchange visits have been needed to address data management issues and resolve unexpected problems. The data management on cross-cultural studies can be handled successfully, given careful planning.

  3. Pure Titanium Membrane (Ultra – Ti®) in the Treatment of Periodontal Osseous Defects: A Split-Mouth Comparative Study

    Science.gov (United States)

    Khanna, Rajeev; Pardhe, Nilesh Dinesh; Srivastava, Nancy; Bajpai, Manas; Gupta, Shailendra

    2016-01-01

    Introduction Although many different types of Guided Tissue Regeneration (GTR) membranes (resorbable/non-resorbable, including titanium mesh) have been used in the field of Periodontics till now, but this is the first and only clinical study testing the effectiveness of an ultra thin pure Titanium Membrane (Ultra Ti) as a GTR membrane in infra-bony periodontal defects. Aim To compare the efficacy of GTR in intra-bony defects with newly introduced non-resorbable barrier membrane, made of titanium called “Ultra-Ti ® GTR Membrane” versus open flap debridement. Materials and Methods A prospective, randomized, controlled, clinical split mouth study was designed wherein each patient received both the control and test treatment. Two similar defects were selected in each of the 12 patients and were randomly assigned to one of the two treatments. Both the surgeries consisted of identical procedures except for the omission of the barrier membrane in the control sites. Full mouth Plaque Index (PI), Gingival Index (GI), Pocket Probing Depth (PPD) and Relative Attachment Level (RAL) were recorded before surgery and after 6 months and 9 months along with hard tissue measurements at the time of surgery and then at re-entry after 9 months. Radiographs were also taken before surgery and 9 months post operatively. Student’s paired t-test and unpaired t-test (SPSS software version 9) were used to analyze the results. Results Nine months after treatment, the test defects gained 4.375 ± 1.189mm of RAL, while the control defects yielded a significantly lower RAL gain of 3.417 ± 0.996mm. Pocket reduction was also significantly higher in the test group (4.917 ± 0.996mm) when compared with the controls (3.83 ± 0.718mm). There was a significant bone fill (54.69% of defect fill) obtained in the test site, unlike the control site (8.91%). Conclusion The present study demonstrated that GTR with “Ultra-Ti® GTR Membrane” resulted in a significant added benefit in comparison with

  4. Studies on the formation of long-chain dicarboxylic acids from pure n-alkanes by a mutant of Canadida tropicalis

    Energy Technology Data Exchange (ETDEWEB)

    Hill, F.F.; Venn, I.; Lukas, K.L.

    1986-05-01

    Individual n-alkanes, from C/sub 11/-C/sub 16/, were metabolized by a mutant of Candida tropicalis. This strain was selected for its inability to grow in the presence of dodecanedioic acid and dodecane as the sole carbon source. Transformations were studied in fed-batch cultures. Undecane was only poorly transformed, but from dodecane to hexadecane high transformation yields were achieved. Maximum yield of acid-precipitable long-chain dioic acids was obtained with tridecane as substrate. All the products were mixtures of different acids. Besides the ..cap alpha..,..omega..-alkanedioic acids, the 3-hydroxy derivatives of longchain ..cap alpha..,..omega..-alkanedioic acids and dioic acids with a shortened carbon chain were found.

  5. Culture Studies in the Field of International Business Research

    DEFF Research Database (Denmark)

    Worm, Verner; Li, Xin; Jakobsen, Michael

    Purpose: The purpose of this paper is to describe the status of culture studies within the field of international business research, and to examine how two main paradigms – essentialism and social constructivism – relate to the discourse in this field. We analyze the main points of the two...... paradigms, and discuss how the relationship between them has evolved. Findings: We show that essentialism and social constructivism have a paradoxical relationship. They seem to be mutually exclusive, yet each offers unique insights into the notion of culture. Both are necessary for a complete understanding...... for the first time that Bohr’s complementarity principle is illustrative and useful for understanding the paradoxical relationship between essentialism and social constructivism. Inspired by two principles enunciated by Bohr – that of complementarity and that of classical concepts – we argue that the two...

  6. Dual Target Search is Neither Purely Simultaneous nor Purely Successive.

    Science.gov (United States)

    Cave, Kyle R; Menneer, Tamaryn; Nomani, Mohammad S; Stroud, Michael J; Donnelly, Nick

    2017-08-31

    Previous research shows that visual search for two different targets is less efficient than search for a single target. Stroud, Menneer, Cave and Donnelly (2012) concluded that two target colours are represented separately based on modeling the fixation patterns. Although those analyses provide evidence for two separate target representations, they do not show whether participants search simultaneously for both targets, or first search for one target and then the other. Some studies suggest that multiple target representations are simultaneously active, while others indicate that search can be voluntarily simultaneous, or switching, or a mixture of both. Stroud et al.'s participants were not explicitly instructed to use any particular strategy. These data were revisited to determine which strategy was employed. Each fixated item was categorised according to whether its colour was more similar to one target or the other. Once an item similar to one target is fixated, the next fixated item is more likely to be similar to that target than the other, showing that at a given moment during search, one target is generally favoured. However, the search for one target is not completed before search for the other begins. Instead, there are often short runs of one or two fixations to distractors similar to one target, with each run followed by a switch to the other target. Thus, the results suggest that one target is more highly weighted than the other at any given time, but not to the extent that search is purely successive.

  7. The Continuing Importance of the "Cultural" in the Study of Youth

    Science.gov (United States)

    Bennett, Andy

    2011-01-01

    The academic study of youth culture has changed markedly in the past two decades. The early 1990s saw a "cultural turn" as the sociological focus moved from "institutional and structural features of society to the study of culture". Andy Bennett begins this article with a critical evaluation of the "cultural turn" and its impact on the field of…

  8. The Cultural Adaptation Process during a Short-Term Study Abroad Experience in Swaziland

    Science.gov (United States)

    Conner, Nathan W.; Roberts, T. Grady

    2015-01-01

    Globalization continuously shapes our world and influences post-secondary education. This study explored the cultural adaptation process of participants during a short-term study abroad program. Participants experienced stages which included initial feelings, cultural uncertainty, cultural barriers, cultural negativity, academic and career growth,…

  9. 纯铜化学镀Ni-Cu-P工艺的研究%A Study of Electroless Ni-Cu-P Plating on Pure Copper

    Institute of Scientific and Technical Information of China (English)

    陈益兵; 赵芳霞; 张振忠; 孙晓东

    2013-01-01

    Aiming at the problems of corrosion and boundary conductivity to be improved when pure copper is in the process of application, the surface of pure copper was modified by electroless Ni-Cu-P plating. The effects of electroless Ni-Cu-P plating process on the properties of pure copper were investigated by means of XRD, SEM, contact resistance test, corrosion test, etc.The results show that the process can obtain a typical amorphous cellular structure, the contact resistance of modified pure copper is 15 % ~ 30 % of that before modification, and the self-corrosion current density is decreased by two orders of magnitude.%针对纯铜使用过程中表面腐蚀及导电性需进一步提高等问题,采用化学镀Ni-Cu-P对纯铜进行表面改性.采用X射线衍射仪(XRD)、扫描电子显微镜(SEM)、界面接触电阻测试、极化曲线等手段,研究了化学镀Ni-Cu-P工艺对纯铜性能的影响.结果表明:该工艺能得到典型的非晶胞状结构,改性后纯铜的接触电阻为改性前的15 %~30%,自腐蚀电流密度降低两个数量级.

  10. Controllability study on the preparation of pure phase BiFeO{sub 3} thin films by liquid phase self-assembled method

    Energy Technology Data Exchange (ETDEWEB)

    Ren Xuanru, E-mail: renxuanru1986@163.com [C/C Composites Research Center, State Key Laboratory of Solidification Processing, Northwestern Polytechnical University, Xi' an, Shaanxi 710072 (China); Tan Guoqiang; Miao Hongyan; Li Ziyu [College of Material Science and Engineering, Shaanxi University of Science and Technology, Xi' an, Shaanxi 710021 (China)

    2012-08-01

    Pure phase BiFeO{sub 3} (BFO) thin films were prepared on the ITO/glass substrates covered with functionalized OTS self-assembled monolayers (SAMs) by controllable liquid phase self-assembled method. The hydrophobic surface of OTS-SAMs was changed into hydrophilic surface after UV irradiation, which is helpful to make BFO precursor solutions fully wet the substrate surface. A dense film was formed only on the hydrophilic silnaol group regions, which shows the selectively deposition of BFO precursors. Changing the pH value of BFO precursor solutions will affect the phase purity. The pure phase BFO thin films can be obtained under various pH values, which indicates that the liquid phase self-assembled method is controllable. All pure phase BFO films are dense, smooth, well-grown polycrystalline films, but the size of grains increased gradually along with the increase of pH values. The micropattern of BiFeO{sub 3} film has clear edges. The possible growth mechanism of BFO thin films was discussed.

  11. A General Study of the Wine Culture in China and the Western World

    Institute of Scientific and Technical Information of China (English)

    WEI Na

    2016-01-01

    Wine culture is considered as an indispensible part of the culture in different countries. Distinctive differences exist be-tween Chinese wine culture and the wine culture in western countries due to their historical backgrounds, different values, cul-tures and customs, etc. A general study of the comparison between Chinese wine culture and western wine culture is given in this paper in order to help us have a better understanding of the wine culture and make us be aware of showing mutual respect for each other.

  12. Studying Linguistic and Cultural Contact in Borneo: Prospects and Challenges

    Directory of Open Access Journals (Sweden)

    Antonia Soriente

    2014-11-01

    Full Text Available In the variegated landscape of languages and cultures of Borneo, the study of languages is a powerful tool to shed light on the intricate history of relations that has long been obscured by the polarization between ‘Dayak’ and ‘Malay’. This article looks at some of the features of Lebu’ Kulit Kenyah, Penan Benalui, Punan Tubu’ and Ma’ Pnaan (Punan Malinau/Segah languages to clarify the linguistic and cultural affiliations among groups that were otherwise lumped together in vague classifications. It demonstrates what is to follow: according to a number of phonological, morphological and lexical evidence, and other historical evidence, Lebu’ Kulit has to be listed among the Kayanic languages. Penan Benalui, like the other Penan languages, is not a Kenyah language, whereas Punan Tubu’, despite the alleged cultural and social similarity with other Punan groups, cannot be classified within the Penan branch nor with other Punan languages. Ma’ Pnaan or Punan Malinau/Segah is not a Punan language and is linguistically classified within the Kayanic branch of the Kayan-Kenyah subgroup.

  13. Twentieth-Century Latin American Literary Studies and Cultural Autonomy

    Directory of Open Access Journals (Sweden)

    Naomi Lindstrom

    1995-06-01

    Full Text Available Since the 1920s, when scholars first began to specialize in Latin American writing, the subject of Latin American literary studies has grown from a small subset of Spanish and Portuguese literary research and teaching to become the largest field within Hispanism and a significant presence in comparative literature. The expansion of their place in the academic world has often prompted students of Latin American literature to wonder whether, in being swept into the mainstream, their field has not left out of account the historical situations of Latin American nations. These reflections lead critics back to a problem that has troubled Latin American thinkers since Independence: the achievement, or erosion, of cultural autonomy. Though undeniably close to major powers, the Latin American nations are unequal partners in trade and cultural exchange. Corresponding to their uneven and shifting relations with Europe and later the United States, their cultural life evolves following a distinctive historical dynamic. This article considers recent efforts by scholars and essayists to characterize the features that distinguish Latin America from more politically and economically advantaged nations. Special attention goes to those scholars who, drawing on anthropological research, examine communicative and expressive practices of indigenous origin, and those who borrow from economic theory to view Latin America as shaped by its history of dependence on more powerful nations and regions.

  14. Recent trends in IBA for cultural heritage studies

    Energy Technology Data Exchange (ETDEWEB)

    Beck, Lucile, E-mail: lucile.beck@cea.fr

    2014-08-01

    Ion beam analysis (IBA) techniques play an important role in the field of cultural heritage. IBA was one of the first physical methods applied to archaeology in the 70s. 40 years later, more than 25 accelerator laboratories were or are involved in cultural heritage applications. The advantages of the IBA techniques match very well the requirements for the analysis of archaeological or museum objects: non destructiveness, major, minor and trace element analysis, information in depth, elemental mapping… Thanks to this collected information, IBA techniques can answer archaeological and conservation questions: provenance determination, ancient technologies (fabrication, transformation, recipes…) and conservation issues. In spite of the competition with portable devices and large facilities (such as synchrotron or neutron reactors), the IBA methods keep their interest due to the possibility of associating two or three of them according to the archaeological or art issue. In this article, some examples chosen for their original approach are presented: PIXE for X-radiography, provenance of prehistoric pigments and painting characterisation. They illustrate the useful versatility of IBA for cultural heritage studies. Perspectives for further improvements are also proposed.

  15. Are all maximally entangled states pure?

    CERN Document Server

    Cavalcanti, D; Terra-Cunha, M O

    2005-01-01

    In this Letter we study if all maximally entangled states are pure through several entanglement monotones. Our conclusions allow us to generalize the idea of monogamy of entanglement. Then we propose a polygamy of entanglement, which express that if a general multipartite state is maximally entangled it is necessarily factorized by any other system.

  16. Training on the DSM-5 Cultural Formulation Interview improves cultural competence in general psychiatry residents: A pilot study.

    Science.gov (United States)

    Mills, Stacia; Xiao, Anna Q; Wolitzky-Taylor, Kate; Lim, Russell; Lu, Francis G

    2017-04-01

    The objective of this study was to assess whether a 1-hour didactic session on the DSM-5 Cultural Formulation Interview (CFI) improves the cultural competence of general psychiatry residents. The main hypothesis was that teaching adult psychiatry residents a 1-hour session on the CFI would improve cultural competence. The exploratory hypothesis was that trainees with more experience in cultural diversity would have a greater increase in cultural competency scores. Psychiatry residents at a metropolitan, county hospital completed demographics and preintervention questionnaires, were exposed to a 1-hour session on the CFI, and were given a postintervention questionnaire. The questionnaire was an adapted version of the validated Cultural Competence Assessment Tool . Paired samples t tests compared pre- to posttest change. Hierarchical linear regression assessed whether pretraining characteristics predicted posttest scores. The mean change of total pre- and posttest scores was significant ( p = .002), as was the mean change in subscales Nonverbal Communications ( p < .001) and Cultural Knowledge ( p = .002). Demographic characteristics did not predict higher posttest scores (when covarying for pretest scores). Psychiatry residents' cultural competence scores improved irrespective of previous experience in cultural diversity. More research is needed to further explore the implications of the improved scores in clinical practice.

  17. Anaerobic culture by Total Air Barrier: A preliminary study

    Directory of Open Access Journals (Sweden)

    Soma Sarkar

    2010-03-01

    Full Text Available BackgroundFor study with obligate anaerobes, inoculated platescontaining suitable reduced media need handling andincubation under strict anaerobic condition. Instead ofensuring a confined oxygen free chamber for placing seededplates, same purpose may be achieved by creating total airbarrier to the surface.MethodUpper moist surface of freshly prepared anaerobic media inPetri plates were intimately covered with very thintransparent bacteriological inert sterile polyester sheets.Stock culture of Bacteroides fragilis, ATCC 23745 andClostridium sporogenes, ATCC 11437 were grown in cookedmeat broth and then sub-cultured on respective plates, afterlifting the cover sheets. Sheets were again covered andincubated at 37oC ordinary incubator. To performantimicrobial susceptibility test, similarly covered seededplates with well inoculums were inverted en-block afterstripping sides with the help of a spatula. Now antibiotic diskswere placed on upper bare surfaces. After short pre-diffusion,plates were incubated keeping inoculated surface below.Same study was performed by conventional method usingGaspak.ResultsGood growths were noted in both sets of the study;however discrete colonies appeared more flat in nature intest set. Almost identical zones of inhibition were noted inboth sets of sensitivity study. Seven days old growths incovered blood agar plates were found viable when subculturedin cooked meat broths.ConclusionIsolation, identification and susceptibility study for mostclinically important obligate anaerobes may be performedby simple barrier method after appropriatestandardization.

  18. Blood CoQ10 levels and safety profile after single-dose or chronic administration of PureSorb-Q40: animal and human studies.

    Science.gov (United States)

    Nukui, Kazuki; Yamagishi, Toshihiko; Miyawaki, Hiromi; Kettawan, Aikkarach; Okamoto, Tadashi; Belardinelli, Romualdo; Tiano, Luca; Littarru, Gian Paulo; Sato, Kiyoshi

    2008-01-01

    Coenzyme Q10 (CoQ10) is known to be highly hydrophobic and, as such, insoluble in water: this leads to serious inconvenience when trying to incorporate it in food products. Its absorption is also known to be very limited. PureSorb-Q40 (P40) (Water-soluble type CoQ10 powder, CoQ10 content 40 w/w % was developed in order to improve its use with food products and to enhance its absorption. In the present study the absorption of this novel formulation was compared to a conventional lipid soluble CoQ10 by administering both products to rats and humans. Acute, single-administration studies in rats showed that P40 has a higher absorption, compared to lipid soluble CoQ10, both in prandial and fasting states. Similarly, single administration in humans revealed a higher absorption level for P40, taken in the fasting state or together with meals. In the rat study, no adverse effects were observed with P40 at doses up to 2,000 mg/kg in both sexes. In a double-blind, placebo controlled, comparative study conducted on 46 healthy volunteers and randomly divided into two groups, in the group receiving 900~mg of CoQ10 per day, for 4 consecutive weeks, the average level at two weeks was 8.79 +/- 3.34 microg/mL, similar to the corresponding level after 4 weeks (8.33 +/- 4.04 microg/mL). After 2 weeks of washout, serum CoQ10 level decreased to 1.30 +/- 0.49 microg/mL. P40 intake did not cause any significant changes in symptoms and clinical laboratory tests as assessed by physical, hematological, blood biochemical or urinalysis. Clinical examinations also did not reveal any abnormalities. The above blood (serum) CoQ10 level at 2 weeks after start of intake was compared with other reported values. The same dose of CoQ10 (900mg/day), when administered by softgel capsules yielded a plasma CoQ10 concentration of 3.6 microg/mL, while P40 levels were 8.79 +/- 3.34 microg/mL. These levels are remarkably high for instance when compared to the corresponding levels obtained, in patients

  19. World Culture and ICT use – a Study from Bhutan

    DEFF Research Database (Denmark)

    Zander, Pär-Ola Mikael; Choeda, Choeda; Dukpa, Dorji

    2015-01-01

    understanding in international projects and global appropriation of technology may be larger than the current theorizing implies. In this paper we investigate the ICT use, with emphasis on World Culture, within Higher Education in Bhutan. We find a remarkably strong presence of World Culture among faculty......The HCI community has recognized the importance of culture and to be culturally sensitive when designing interaction. Local culture should not be overlooked when designing. On the other hand, recent advances in theorizing of cultural patterns have reported that in some sectors, World Cultures has...... permeated institutions across the world. Such cultures can be said to consist of a dynamic system of values, beliefs, behaviours, norms, rules, tools and technologies, and show similar patterns across the world. If such a culture is present to the degree argued by World Culturalists, the scope for mutual...

  20. Bovine lactotroph cultures for the study of prolactin synthesis functions.

    Science.gov (United States)

    Wang, Jianfa; Yang, Zhanqing; Fu, Shoupeng; Liu, Bingrun; Wu, Dianjun; Wang, Wei; Sun, Dongbo; Wu, Rui; Liu, Juxiong

    2016-03-01

    The aim of this study was to establish a bovine anterior pituitary-derived lactotroph (BAPDL) line that expresses prolactin (PRL) in vitro to study the mechanisms of bovine PRL synthesis and secretion. Immunohistochemistry assay of PRL in the newborn calves' anterior pituitary glands showed that most lactotrophs were located within the superior border of the lateral wings of the anterior pituitary. Tissues of the superior border of the lateral wings of the anterior pituitary were dispersed and cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS). The limiting dilution method was used to establish BAPDL from single cell clone. BAPDL cells constantly expressed mRNAs for PRL and pituitary-specific transcription factor 1 (Pit-1) gene and grew steadily and rapidly in the DMEM supplemented with 10% FBS. PRL immunoreactivity was present in BAPDL at passage 20. The concentration of bovine PRL in BAPDL at passage 20 culture supernatant was decreased to below 35% compared with that in BAPDL at passage 1. The effects of human epidermal growth factor (hEGF) and dopamine (DA) on the expression and secretion of PRL in BAPDL at passage 4 were also investigated. The results are consistent with those of previous studies. Thus, it can be used successfully for studying the mechanisms of stimuli regulating PRL synthesis and release.

  1. Chalcone dimethylallyltransferase from Morus nigra cell cultures. Substrate specificity studies.

    Science.gov (United States)

    Vitali, Alberto; Giardina, Bruno; Delle Monache, Giuliano; Rocca, Filippo; Silvestrini, Andrea; Tafi, Andrea; Botta, Bruno

    2004-01-16

    A new prenyltransferase (PT) enzyme derived from the microsomal fractions of cell cultures of Morus nigra was shown to be able to prenylate exclusively chalcones with a 2',4'-dihydroxy substitution and the isoflavone genistein. Computational studies were performed to shed some light on the relationship between the structure of the substrate and the enzymatic activity. PT requires divalent cations, particularly Mg(2+), to be effective. The apparent K(m) values for gamma,gamma-dimethylallyldiphosphate and 2',4'-dihydroxychalcone were 63 and 142 microM, respectively. The maximum activity of the enzyme was expressed during the first 10 days of cell growth.

  2. Buddhism, Poststructuralist Thought, Cultural Studies: A Profession of Faith

    Directory of Open Access Journals (Sweden)

    Edwin Ng

    2012-09-01

    Full Text Available This essay is a result of the author's research into Buddhism and poststructuralist theories on knowledge, self and ethics and attempts to articulate the ethico-political implications of his own practice of Vipassana (a form of Buddhist meditation and interrogate what is called the politics of spirituality. He explores the role of faith within cultural studies by first contextualising the relevant discursive fields, then  analysing Vipassana with a ‘religious’ Foucauldian approach oriented around the critico-political aims of Foucault's late work. This elucidates how Buddhist and poststructuralist thought share certain concerns, and identifies trajectories for further inquiry.

  3. Cultural Studies og kritisk diskurs analyse i 8. klasse

    DEFF Research Database (Denmark)

    Svarstad, Lone Krogsgaard

    2015-01-01

    to be multiplicative and dynamic and power is foregrounded. Critical discourse analysis is used as a tool in class to analyse a dispute on Facebook between Miley Cyrus and Sinead O’Connor. Students work with active construction, making connections, social interaction, reflection, responsibility and they do so...... thesis in the school year 2013-2014.The approach is based on Cultural Studies and knowledge of social categories and representation, and how these can be taught and discussed in class through an intersectional lens. An intersectional lens allows for the conceptualization of categories...

  4. Culture Industry Development and Regional Economy - Case Study of Tianjin*

    Science.gov (United States)

    Shu-sheng, Li

    Cultural industry is one of the fastest growing industries and the added value of culture industry is almost more than 10% of GDP in developed countries. This paper first introduced the boundaries of cultural industries and the development of that in Tianjin. Then the logistic growth model was used to detect the stage of the life cycle of culture industry in Tianjin. We draw the conclusion that culture industry is in the rapid growth stage in Tianjin, and we must take scientific development, increasing innovation through structural reform, technological advances, personnel training and other measures to promote the cultural industry development in Tianjin by leaps.

  5. Relativity of pure states entanglement

    CERN Document Server

    Zyczkowski, K; Zyczkowski, Karol; Bengtsson, Ingemar

    2002-01-01

    Entanglement of any pure state of an N times N bi-partite quantum system may be characterized by the vector of coefficients arising by its Schmidt decomposition. We analyze various measures of entanglement derived from the generalized entropies of the vector of Schmidt coefficients. For N >= 3 they generate different ordering in the set of pure states and for some states their ordering depends on the measure of entanglement used. This odd-looking property is acceptable, since these incomparable states cannot be transformed to each other with unit efficiency by any local operation. In analogy to special relativity the set of pure states equivalent under local unitaries has a causal structure so that at each point the set splits into three parts: the 'Future', the 'Past' and the set of noncomparable states.

  6. Organ culture-cell culture system for studying multistage carcinogenesis in respiratory epithelium. [Mice

    Energy Technology Data Exchange (ETDEWEB)

    Steele, Vernon E.; Marchok, Ann C.; Nettesheim, Paul

    1977-01-01

    An organ culture-cell culture system was used to demonstrate carcinogen dose-dependent transformation of tracheal epithelial cells in vitro. Tracheal explants were exposed to MNNG (N-methyl-N/sup 1/-nitro-N-nitrosoguanidine) in organ culture. Outgrowths from these explants provided epithelial cell cultures. The numbers of long term epithelial cell cultures and cell lines that were established per explant increased as MNNG exposure concentration increased. At the present time, more cell lines derived from explants exposed to the highest MNNG concentration have produced palpable tumors than cell lines derived from explants exposed to lower MNNG concentrations. No cell lines were established from primaries derived from control explants. TPA (12-0-tetradecanoyl-phorbol-13-acetate), stimulates DNA synthesis in tracheal epithelium in organ culture in a manner simular to that described for mouse skin. Short exposures to TPA not only stimulated DNA synthesis earlier, but the stimulation was greater than that obtained with continuous exposure. At the present time, exposure of tracheal organ cultures to MNNG followed by TPA has resulted in an enhanced production of morphologically altered cells in primary epithelial cell cultures, than exposure to either agent alone.

  7. Characteristic and Cultural Study of the Politeness in Chinese and English Cultures

    Institute of Scientific and Technical Information of China (English)

    席晓

    2008-01-01

    s This paper shows that people lays special emphasis on difference aspects of politeness principle in the light of their own culture in cross-communication. And it analyses what the differences of PP in the language us-ing in Pragmatic are, and why? It deals with the differences between English and Chinese cultures.

  8. Education Abroad and Domestic Cultural Immersion: A Comparative Study of Cultural Competence among Teacher Candidates

    Science.gov (United States)

    Hare Landa, Melissa; Odòna-Holm, Jocelyn; Shi, Lijuan

    2017-01-01

    As the demographics of the United States continue to shift, American classrooms reflect the richness of cultural diversity and the vibrancy of immigrant populations. Education abroad programs provide opportunities for preservice teachers to develop their cultural competence, required for effectively teaching children from a range of cultural…

  9. Marxism in Vygotskian approaches to cultural studies of science education

    Science.gov (United States)

    Lima Junior, Paulo; Ostermann, Fernanda; Rezende, Flavia

    2014-09-01

    In this paper we initially address the main categories of Marxism, illustrating how Vygotsky has appropriated them as mediational meta-theoretical tools for building concepts for his psychological approach. In order to investigate the influence of Marxism in cultural studies of science education, we make an account of how current research, sustained by Vygotsky's original and successor theories, has been appropriating meta-theoretical categories of dialectical materialism. Once we identified Cultural Studies of Science Education as a journal that would probably concentrate papers that follow these perspectives, we decided to take it as the context of this study. In the process of selecting the corpus to be reviewed from the editions published from 2006 to 2011, we have found that 16 % of the articles that matched keywords denoting frameworks related to the Vygotskian tradition developed and appropriated the categories of dialectical materialism. The quality and originality of contemporary development of CHAT denote that this framework has been playing a very important role in recent expansion of Vygotskian approaches to research in science education. Among the papers that we considered to develop and appropriate Vygotskian frameworks, incompletion in the appropriation of meta-theoretical categories of dialectical materialism and the misusage of dialectics intertwined with dialogism were highlighted. Our findings suggest that overcoming these limitations can enhance political analysis of sociocultural phenomena in the context of science education. It also represents a strengthening of the role of dialectical materialism in expanding sociocultural perspectives toward a better articulation between individual and institutional-centered analyses.

  10. Assessment of Military Cultural Competence: A Pilot Study.

    Science.gov (United States)

    Meyer, Eric G; Hall-Clark, Brittany N; Hamaoka, Derrick; Peterson, Alan L

    2015-08-01

    Cultural competence is widely considered a cornerstone of patient care. Efforts to improve military cultural competency have recently gained national attention. Assessment of cultural competence is a critical component to this effort, but no assessment of military cultural competence currently exists. An assessment of military cultural competence (AMCC) was created through broad input and consensus. Careful review of previous cultural competency assessment designs and analysis techniques was considered. The AMCC was organized into three sections: skills, attitudes, and knowledge. In addition to gathering data to determine absolute responses from groups with different exposure levels to the military (direct, indirect, and none), paired questions were utilized to assess relative competencies between military culture and culture in general. Piloting of the AMCC revealed significant differences between military exposure groups. Specifically, those with personal military exposure were more likely to be in absolute agreement that the military is a culture, were more likely to screen for military culture, and had increased knowledge of military culture compared to those with no military exposure. Relative differences were more informative. For example, all groups were less likely to agree that their personal culture could be at odds with military culture as compared to other cultures. Such perceptions could hinder asking difficult questions and thus undermine care. The AMCC is a model for the measurement of the skills, attitudes, and knowledge related to military cultural competence. With further validity testing, the AMCC will be helpful in the critical task of measuring outcomes in ongoing efforts to improve military cultural competence. The novel approach of assessing variance appears to reduce bias and may also be helpful in the design of other cultural competency assessments.

  11. A Brief Study on Culture Shock over Overseas Students and the Solution to New Cultural Environments

    Institute of Scientific and Technical Information of China (English)

    张维

    2014-01-01

    Culture shock is one of the common experiences by students and people abroad. They may experience the feeling of anxiety, disorientation, confusion, and even hostility because of misunderstanding to each other.“Culture shock”refers to the phenomenon in which people engage in the interaction feel depressed, discomfortable, etc. Culture shock may be caused by the conflict of various value systems when students and people enter a new culture and other value system. Culture shock is also a consequence of the influence of the negative life event. The changes in one ’s life and lack of support from family members, friends will make them more vulnerable and cause great discomfort in the new environment.

  12. Pure Spinors for General Backgrounds

    CERN Document Server

    Fre', Pietro

    2008-01-01

    We show the equivalence of the different types of pure spinor constraints geometrically derived from the Free Differential Algebras of N=2 d=10 supergravities. Firstly, we compute the general solutions of these constraints, using both a G_2 and an SO(8) covariant decomposition of the 10d chiral spinors. Secondly, we verify that the number of independent degrees of freedom is equal to that implied by the Poincare' pure spinor constraints so-far used for superstrings, namely twenty two. Thirdly, we show the equivalence between the FDA type IIA/B constraints among each other and with the Poincare' ones.

  13. 改性足金材料的耐蚀性能研究%Study on corrosion resistance of modified pure gold materials

    Institute of Scientific and Technical Information of China (English)

    李桂双; 陈燕兰; 袁军平; 陈绍兴; 卢焕洵

    2015-01-01

    传统足金/Au999在黄金首饰消费中占据了最大份额,它们具有高贵的黄色和优异的耐蚀性,但硬度过低的问题制约了饰品的艺术价值,也给制造和使用带来了问题。该文采用钴、锑、钪对足金进行了多元微合金化处理,并采用人工汗液浸泡法和电化学试验法检测了合金的腐蚀倾向和抗变色性能。试验结果表明:改性足金的初始颜色与Au999之间的色差仅处于肉眼可觉察的区间,它在人工汗液中的颜色变化率与其所处状态有关,呈现“形变时效态>固溶态>冷形变态”的顺序。改性足金在人工汗液中存在钝化行为,耐点蚀性能与Au999相当,表现出优良的抗变色性能。%Traditional pure gold/Au999 jewelry has the noble yellow glows and excellent corrosion resistance and accounts for the biggest share in gold jewelry consumption,however,its low hardness restricts the artistic value and brings problems in manufacture and application.In this paper,pure gold was multi-alloyed with cobalt,antimony and scandium,and the corrosion tendency and the anti-chromism property of the modified gold were tested by artificial sweat soaking and electrochemical method.The results show that the original chromatic aberration of the modified gold from pure gold is only discernible between the range of naked eye,and the color change rate after soaking in artificial sweat is related to its state,which shows the order"cold working and aging state>solid solution state>cold working state".The modified gold presents passivation behavior in artificial sweat,its pitting corrosion resistance is close to pure gold and shows excellent discoloration resistance.

  14. Study on the Stability of high pure Chiorogenic Acid%高纯度绿原酸的稳定性研究

    Institute of Scientific and Technical Information of China (English)

    涂北平; 赖卫华

    2011-01-01

    Objective:To investigate the stability of high pure chlorogenic acid in different conditions. Method:The stability of high pure chlorogenic acid in different antioxidants( sodium hydrogensulfite 、sodium pymsulfite 、L- cysteine monohydrochloride 、 ascorbic acid ), phosptat buffe with different pH values, organic solvents ( methanol 、ethanol), copper ions and complexing agent and β - cyclodextrins was investigated by the classical isothermal method. Result: The experiments showed the high pure chlorogenic acid was the most stable in L - cysteine monohydrochloride than in others antioxidants. It was more stable in acidic water than in basic water. It was stable in these organic solvents. The copper ions and complexing agents have certain stimulative effect on the stability of chlorogenic acid. Conclusion: In different conditions, the stability of high pure chlorogenic acid was different. It prodded a reference to the extraction and analysis of chlorogenic acid and production of chlorogenic acid preparation.%目的:研究高纯度绿原酸在不同条件下的稳定性。方法:采用高纯度绿原酸在抗氧化剂(亚硫酸氢钠、焦亚硫酸钠、L一半胱胺酸盐、维生素c)、不同pH值磷酸盐缓冲液、有机溶剂(甲醇、乙醇)、金属铜离子及络合剂及13一环糊精中的稳定性。结果:绿原酸在抗氧化剂L一半胱胺酸盐中最稳定,在酸性环境较碱性环境中更稳定,在上述有机溶剂中稳定,在金属铜离子与络合剂复合体对绿原酸影响稳定性有一定的促进作用。结论:高纯度绿原酸在不同条件下稳定性不同,该结果可为绿原酸的提取、分析及其相关制剂的生产提供参考。

  15. Associations of fats and carbohydrate intake with cardiovascular disease and mortality in 18 countries from five continents (PURE): a prospective cohort study.

    Science.gov (United States)

    Dehghan, Mahshid; Mente, Andrew; Zhang, Xiaohe; Swaminathan, Sumathi; Li, Wei; Mohan, Viswanathan; Iqbal, Romaina; Kumar, Rajesh; Wentzel-Viljoen, Edelweiss; Rosengren, Annika; Amma, Leela Itty; Avezum, Alvaro; Chifamba, Jephat; Diaz, Rafael; Khatib, Rasha; Lear, Scott; Lopez-Jaramillo, Patricio; Liu, Xiaoyun; Gupta, Rajeev; Mohammadifard, Noushin; Gao, Nan; Oguz, Aytekin; Ramli, Anis Safura; Seron, Pamela; Sun, Yi; Szuba, Andrzej; Tsolekile, Lungiswa; Wielgosz, Andreas; Yusuf, Rita; Hussein Yusufali, Afzal; Teo, Koon K; Rangarajan, Sumathy; Dagenais, Gilles; Bangdiwala, Shrikant I; Islam, Shofiqul; Anand, Sonia S; Yusuf, Salim

    2017-08-28

    The relationship between macronutrients and cardiovascular disease and mortality is controversial. Most available data are from European and North American populations where nutrition excess is more likely, so their applicability to other populations is unclear. The Prospective Urban Rural Epidemiology (PURE) study is a large, epidemiological cohort study of individuals aged 35-70 years (enrolled between Jan 1, 2003, and March 31, 2013) in 18 countries with a median follow-up of 7·4 years (IQR 5·3-9·3). Dietary intake of 135 335 individuals was recorded using validated food frequency questionnaires. The primary outcomes were total mortality and major cardiovascular events (fatal cardiovascular disease, non-fatal myocardial infarction, stroke, and heart failure). Secondary outcomes were all myocardial infarctions, stroke, cardiovascular disease mortality, and non-cardiovascular disease mortality. Participants were categorised into quintiles of nutrient intake (carbohydrate, fats, and protein) based on percentage of energy provided by nutrients. We assessed the associations between consumption of carbohydrate, total fat, and each type of fat with cardiovascular disease and total mortality. We calculated hazard ratios (HRs) using a multivariable Cox frailty model with random intercepts to account for centre clustering. During follow-up, we documented 5796 deaths and 4784 major cardiovascular disease events. Higher carbohydrate intake was associated with an increased risk of total mortality (highest [quintile 5] vs lowest quintile [quintile 1] category, HR 1·28 [95% CI 1·12-1·46], ptrend=0·0001) but not with the risk of cardiovascular disease or cardiovascular disease mortality. Intake of total fat and each type of fat was associated with lower risk of total mortality (quintile 5 vs quintile 1, total fat: HR 0·77 [95% CI 0·67-0·87], ptrendsaturated fat, HR 0·86 [0·76-0·99], ptrend=0·0088; monounsaturated fat: HR 0·81 [0·71-0·92], ptrendfat: HR 0·80

  16. Factors contributing to the rheology of tomato puree

    OpenAIRE

    Abson, Rachael

    2013-01-01

    Tomato (Solanum Iycopersicum) puree is created by homogenising the flesh of tomato fruits. The viscosity of this material and the fibrous content is of commercial interest. Tomato puree consists of suspended particles (consisting of whole cells, broken cells and cellular fragments) in an aqueous serum. The contribution of the non-soluble and soluble material to tomato puree rheology was studied with reference to the varying composition of solids and firmness of tomato fruit at four stages of ...

  17. Eco-Cultural Tourism: A Tool for Environmental, Cultural and Economic Sustainability (A Case Study of Darap Village, West Sikkim

    Directory of Open Access Journals (Sweden)

    Cajee Laitpharlang

    2014-01-01

    Full Text Available Eco-cultural tourism is a concept where both ecological and cultural aspects of a landscape are combined together creating a tourist paradise. It is travel to destinations where both cultural and natural endowments are the prime attractions and thereby considered to be a potential strategy to support conservation of natural habitats along with economic sustainability particularly to indigenous communities. North Eastern Region of India is one of the most mesmerizing regions of the Indian subcontinent that mirrors a perfect blending of life, culture and ecology. It is a relatively unexplored and unique area in terms of both ecology and cultural diversity and is characterized by the abundance of natural endowments; pristine forests with a huge diversity of flora and fauna, enchanting hills, fast flowing streams and meandering rivers, cascading waterfalls, snowcapped mountain ranges etc. Approximately there are 65 indigenous tribes in the region along with a host of sub-tribes having their own distinctive cultures, customs and traditions preserved till today. This article tries to argue through a case study of village tourism at Darap in West Sikkim that eco-cultural tourism is a potent force for the overall economic, social and cultural development as well biodiversity conservation in the region.

  18. Analytical techniques applied to study cultural heritage objects

    Energy Technology Data Exchange (ETDEWEB)

    Rizzutto, M.A.; Curado, J.F.; Bernardes, S.; Campos, P.H.O.V.; Kajiya, E.A.M.; Silva, T.F.; Rodrigues, C.L.; Moro, M.; Tabacniks, M.; Added, N., E-mail: rizzutto@if.usp.br [Universidade de Sao Paulo (USP), SP (Brazil). Instituto de Fisica

    2015-07-01

    The scientific study of artistic and cultural heritage objects have been routinely performed in Europe and the United States for decades. In Brazil this research area is growing, mainly through the use of physical and chemical characterization methods. Since 2003 the Group of Applied Physics with Particle Accelerators of the Physics Institute of the University of Sao Paulo (GFAA-IF) has been working with various methodologies for material characterization and analysis of cultural objects. Initially using ion beam analysis performed with Particle Induced X-Ray Emission (PIXE), Rutherford Backscattering (RBS) and recently Ion Beam Induced Luminescence (IBIL), for the determination of the elements and chemical compounds in the surface layers. These techniques are widely used in the Laboratory of Materials Analysis with Ion Beams (LAMFI-USP). Recently, the GFAA expanded the studies to other possibilities of analysis enabled by imaging techniques that coupled with elemental and compositional characterization provide a better understanding on the materials and techniques used in the creative process in the manufacture of objects. The imaging analysis, mainly used to examine and document artistic and cultural heritage objects, are performed through images with visible light, infrared reflectography (IR), fluorescence with ultraviolet radiation (UV), tangential light and digital radiography. Expanding more the possibilities of analysis, new capabilities were added using portable equipment such as Energy Dispersive X-Ray Fluorescence (ED-XRF) and Raman Spectroscopy that can be used for analysis 'in situ' at the museums. The results of these analyzes are providing valuable information on the manufacturing process and have provided new information on objects of different University of Sao Paulo museums. Improving the arsenal of cultural heritage analysis it was recently constructed an 3D robotic stage for the precise positioning of samples in the external beam setup

  19. The challenge of truth telling across cultures: a case study.

    Science.gov (United States)

    Zahedi, Farzaneh

    2011-01-01

    Accompanied with various opinions across cultures, truth telling is a major debate in bioethics. Many studies have focused on attitudes toward truth disclosure. We intend to review several relevant research studies, and discuss the issue through a clinical case consultation. It seems that while "the right to know" is emphasized in bioethics, in some cultural contexts, health professionals fear communicating bad news. The patients may not receive information directly, because it is believed that the truth may make the patient feel hopeless and unable to cope with the problem. Nevertheless, some believe that sharing information may strengthen a trusting relationship between patients and medical professionals. Extensive efforts are in process in some societies to make patient rights to know the truth as a natural part of medical practice. However, in some cases, the principles of respect for patient autonomy require us to accept patient's refusal to know the truth, with the provision that he assigns someone to receive information and make medical decisions on his behalf. In conclusion, it is suggested that healthcare professionals should not act on a unique presumption in all cases and they should explore what the real interest of patient is, in order to respect individual autonomy.

  20. Kinetic study of biohydrogen production in mixed cultures

    Energy Technology Data Exchange (ETDEWEB)

    Chen, W.-H.; Khanal, S.K.; Sung, S. [Iowa State Univ., Ames, IA (United States). Dept. of Civil Construction and Environmental Engineering

    2004-07-01

    Hydrogen is considered to be a clean energy source and a promising alternative to fossil fuels. The production of biohydrogen from renewable feedstocks such as sugars and organic wastes has gained renewed interest in response to energy insecurity and environmental concerns. The main challenge of producing biohydrogen is the low hydrogen conversion efficiency in the dark fermentation process. A better reactor design could solve the problem. A good index for process design is the microbial growth rate during a growth phase, as this has a strong impact on culture productivity. Microbial growth rate is also a good index for maximizing biohydrogen production. Kinetic studies generally identify the significant operating parameters such as maximum specific growth rate and half-saturation constant. However, this traditional approach cannot be used for hydrogen producing systems. This study established an alternative method to determine growth kinetics for hydrogen producing bacteria. Sucrose and nonfat dairy milk were the substrates used to identify the growth kinetics in a series of batch experiments. The parameters can be used to design a continuous reactor system for an enriched culture of hydrogen producing bacteria. 1 fig.

  1. A Study on Pragmatic Failure in Cross-Cultural Communication

    Science.gov (United States)

    Fang, Jie

    2010-01-01

    Through analyzing and comparing the anecdotes of pragmatic failure in cross-cultural communication from the aspects of lexicon, syntax and discourse, some pragmatic strategies are suggested in intercultural communication. To improve learners' cultural awareness and communicative competence, a cultural-linguistic approach in foreign language…

  2. Exploring the simplest purely baryonic decay processes

    CERN Document Server

    Geng, C Q; Rodrigues, Eduardo

    2016-01-01

    We propose to search for purely baryonic decay processes at the LHCb experiment. In particular, we concentrate on the decay $\\Lambda_b^0\\to p\\bar pn$, which is the simplest purely baryonic decay mode, with solely spin-1/2 baryons involved. We predict its decay branching ratio to be ${\\cal B}(\\Lambda_b^0\\to p\\bar pn)=(2.0^{+0.3}_{-0.2})\\times 10^{-6}$, which is sufficiently large to make the decay mode accessible to LHCb. Though not considered in general, purely baryonic decays could shed light on the puzzle of the baryon number asymmetry in the universe by means of a better understanding of the baryonic nature of our matter world. As such, they constitute a yet unexplored class of decay processes worth investigating. Our study can be extended to the purely baryonic decays of $\\Lambda_b^0\\to p\\bar p \\Lambda$, $\\Lambda_b^0\\to \\Lambda \\bar p\\Lambda$ and $\\Lambda_b^0\\to \\Lambda\\bar \\Lambda\\Lambda$, as well as other similar anti-triplet $b$-baryon decays, such as $\\Xi_b^{0,-}$.

  3. Characterization of Streptococcus thermophilus two-component systems: In silico analysis, functional analysis and expression of response regulator genes in pure or mixed culture with its yogurt partner, Lactobacillus delbrueckii subsp. bulgaricus.

    Science.gov (United States)

    Thevenard, Benoît; Rasoava, Niriaina; Fourcassié, Pascal; Monnet, Véronique; Boyaval, Patrick; Rul, Françoise

    2011-12-02

    The lactic acid bacterium Streptococcus thermophilus (S. thermophilus) is widely used in the dairy industry. As a food bacterium, it has to cope with changing environments such as milk, yogurt, as well as the digestive tract, after the product has been ingested. In bacteria, two-component systems (TCS) are one of the most prevalent mechanisms to sense and respond appropriately to a wide range of signals. They are typically composed of a sensor kinase (HK) that detects a stimulus and a response regulator (RR) which acts as a transcriptional regulator. Our objective was to make an inventory of the TCS present in S. thermophilus LMD-9 and investigate the contribution of each TCS to LMD-9 growth in milk. For that purpose, we performed in silico, transcriptomic as well as functional analysis. The LMD-9 genome presented 6 complete TCS with both HK and RR (TCS 2, 4, 5, 6, 7, and 9) and 2 orphan RRs (RR01 and 08) with truncated HK. Our in silico analysis revealed that for 5 TCS out of the 8, orthologs with known functions were found in other bacterial species whereas for TCS02, 4 and 6 the function of the orthologs are unidentified. Transcriptomic studies (using quantitative PCR) revealed that all S. thermophilus LMD-9 response regulator genes were expressed in milk; they were expressed at different levels and with different profiles during growth. In mixed culture with Lactobacillus delbrueckii subsp. bulgaricus (L. bulgaricus), the S. thermophilus partner in yogurt, the expression of four S. thermophilus LMD-9 response regulator increased; two of them, rr02 and rr09, increased by a factor of 6. These results indicate that the presence of L. bulgaricus induces regulatory changes in S. thermophilus. We also demonstrated that a response regulator (rr02) can exert its regulatory function on its target genes even when expressed at very low levels. We showed that RR05-an ortholog of Bacillus subtilis YycF or Staphylococcus aureus WalR-was essential for the growth of S

  4. STUDY ON DIFFERENTIATION OF RATS EMBRYONIC STEM CELLS CULTURED IN BRL-CM INTO NEURAL PRECURSOR CELLS

    Institute of Scientific and Technical Information of China (English)

    张晓智; 李旭; 徐海伟; 陈葳

    2003-01-01

    Objective To investigate whether buffalo rat liver cell-conditioned medium (BRL-CM) can be used as the culture medium of embryonic stem (ES) cells, and to get relatively pure neural precursor cells (NPCs) for treatment aim. Methods Mouse ES cells were cultured in BRL-CM and medium contain leukemia inhibitory factor (LIF), respectively. NPCs were selectively cultured in serum-free medium. Alkaline phosphatase activity was visualized with NBT/BCIP and nestin antigen was detected with immunocytochemical methods. Results BRL-CM could be used as an efficiency culture condition instead of LIF in ES cells culture. About 86% of cells derived from ES cells in the serum-free culture were NPCs. Conclusion BRL-CM can replace LIF to use in ES cell culture. High purity of NPC can be induced from ES cells with serum-free culture method.

  5. Effects of Business Model Development Projects on Organizational Culture: A Multiple Case Study of SMEs

    National Research Council Canada - National Science Library

    Ulla Santti; Tuomo Eskelinen; Mervi Rajahonka; Kaija Villman; Ari Happonen

    2017-01-01

    ...” that helps a company develop organizational culture as a competitive advantage. In this study of three case companies, the organizational culture change due business model development projects is studied using the Competing Values Framework (CVF...

  6. CO Gas Sensing Properties of Pure and Cu-Incorporated SnO₂ Nanoparticles: A Study of Cu-Induced Modifications.

    Science.gov (United States)

    Karthik, Tangirala Venkata Krishna; Olvera, María de la Luz; Maldonado, Arturo; Gómez Pozos, Heberto

    2016-08-15

    Pure and copper (Cu)-incorporated tin oxide (SnO₂) pellet gas sensors with characteristics provoking gas sensitivity were fabricated and used for measuring carbon monoxide (CO) atmospheres. Non-spherical pure SnO₂ nano-structures were prepared by using urea as the precipitation agent. The resultant SnO₂ powders were ball milled and incorporated with a transition metal, Cu, via chemical synthesis method. The incorporation is confirmed by high-resolution transmission electron microscope (HRTEM) analysis. By utilizing Cu-incorporated SnO₂ pellets an increase in the CO sensitivity by an order of three, and a decrease in the response and recovery times by an order of two, were obtained. This improvement in the sensitivity is due to two factors that arise due to Cu incorporation: necks between the microparticles and stacking faults in the grains. These two factors increased the conductivity and oxygen adsorption, respectively, at the pellets' surface of SnO₂ which, in turn, raised the CO sensitivity.

  7. Experimental study and modelling of heat transfer during condensation of pure fluid and binary mixture on a bundle of horizontal finned tubes

    Energy Technology Data Exchange (ETDEWEB)

    Belghazi, M.; Marvillet, C. [Commissariat a l' Energie Atomique, Grenoble (France). Groupement pour la Recherche sur les Echangeurs thermiques; Bontemps, A. [Universite Joseph Fourier, Grenoble (France). LEGI/GRETh

    2003-03-01

    An experimental investigation was conducted to measure the local heat transfer coefficient for each row in a trapezoidal finned horizontal tube bundle during condensation of both pure fluid (HFC 134a) and several compositions of the non-azeotropic binary mixture HFC 23/HFC 134a. The test section is a 13x3 (rows x columns) tube bundle and the heat transfer coefficient is measured using the modified Wilson plot method. The inlet vapour temperature is fixed at 40{sup o}C and the water flow rate in each active row ranges from 170 to 600 l/h. The test series cover five different finned tubes all commercially available, K11 (11 fins/inch), K19 (19 fins/inch), K26 (26 fins/inch), K32 (32 fins/inch), K40 (40 fins/inch) and their performances were compared. The experimental results were checked against available models predicting the heat transfer coefficient during condensation of pure fluids on banks of finned tubes. Modelling of heat exchange during condensation of binary mixtures on bundles of finned tubes based on the curve condensation model is presented. (author)

  8. CO Gas Sensing Properties of Pure and Cu-Incorporated SnO2 Nanoparticles: A Study of Cu-Induced Modifications

    Directory of Open Access Journals (Sweden)

    Tangirala Venkata Krishna Karthik

    2016-08-01

    Full Text Available Pure and copper (Cu-incorporated tin oxide (SnO2 pellet gas sensors with characteristics provoking gas sensitivity were fabricated and used for measuring carbon monoxide (CO atmospheres. Non-spherical pure SnO2 nano-structures were prepared by using urea as the precipitation agent. The resultant SnO2 powders were ball milled and incorporated with a transition metal, Cu, via chemical synthesis method. The incorporation is confirmed by high-resolution transmission electron microscope (HRTEM analysis. By utilizing Cu-incorporated SnO2 pellets an increase in the CO sensitivity by an order of three, and a decrease in the response and recovery times by an order of two, were obtained. This improvement in the sensitivity is due to two factors that arise due to Cu incorporation: necks between the microparticles and stacking faults in the grains. These two factors increased the conductivity and oxygen adsorption, respectively, at the pellets’ surface of SnO2 which, in turn, raised the CO sensitivity.

  9. CO Gas Sensing Properties of Pure and Cu-Incorporated SnO2 Nanoparticles: A Study of Cu-Induced Modifications

    Science.gov (United States)

    Karthik, Tangirala Venkata Krishna; Olvera, María de la Luz; Maldonado, Arturo; Gómez Pozos, Heberto

    2016-01-01

    Pure and copper (Cu)-incorporated tin oxide (SnO2) pellet gas sensors with characteristics provoking gas sensitivity were fabricated and used for measuring carbon monoxide (CO) atmospheres. Non-spherical pure SnO2 nano-structures were prepared by using urea as the precipitation agent. The resultant SnO2 powders were ball milled and incorporated with a transition metal, Cu, via chemical synthesis method. The incorporation is confirmed by high-resolution transmission electron microscope (HRTEM) analysis. By utilizing Cu-incorporated SnO2 pellets an increase in the CO sensitivity by an order of three, and a decrease in the response and recovery times by an order of two, were obtained. This improvement in the sensitivity is due to two factors that arise due to Cu incorporation: necks between the microparticles and stacking faults in the grains. These two factors increased the conductivity and oxygen adsorption, respectively, at the pellets’ surface of SnO2 which, in turn, raised the CO sensitivity. PMID:27537877

  10. Pure robotic retrocaval ureter repair

    Directory of Open Access Journals (Sweden)

    Ashok k. Hemal

    2008-12-01

    Full Text Available PURPOSE: To demonstrate the feasibility of pure robotic retrocaval ureter repair. MATERIALS AND METHODS: A 33 year old female presented with right loin pain and obstruction on intravenous urography with the classical "fish-hook" appearance. She was counseled on the various methods of repair and elected to have a robot assisted repair. The following steps are performed during a pure robotic retrocaval ureter repair. The patient is placed in a modified flank position, pneumoperitoneum created and ports inserted. The colon is mobilized to expose the retroperitoneal structures: inferior vena cava, right gonadal vein, right ureter, and duodenum. The renal pelvis and ureter are mobilized and the renal pelvis transected. The ureter is transposed anterior to the inferior vena cava and a pyelopyelostomy is performed over a JJ stent. RESULTS: This patient was discharged on postoperative day 3. The catheter and drain tube were removed on day 1. Her JJ stent was removed at 6 weeks postoperatively. The postoperative intravenous urography at 3 months confirmed normal drainage of contrast medium. CONCLUSION: Pure robotic retrocaval ureter is a feasible procedure; however, there does not appear to be any great advantage over pure laparoscopy, apart from the ergonomic ease for the surgeon as well the simpler intracorporeal suturing.

  11. Studies of the metabolism of cell cultures by microspectrofluoroscopy

    Science.gov (United States)

    Hoehne, Wolfgang; Schramm, Werner; Moritzen, V.; Burgmann, U.; Kronfeldt, Heinz D.

    1996-01-01

    The monitoring of the state of cellular energy metabolism and respiratory activity is a necessary procedure in cell biology and pharmacology. One method is the observation of the redox state by NADH and FAD autofluorescence measurements. Using this technique, investigations on endothelial cell cultures were done to study their behavior under pharmacologic influences. One application was the investigation of cytotoxicity of cyanides, blocking the mitochondrial respiratory chain. Further we studied the activation of energy metabolism as a step of the cellular reaction on extracellular impacts. The measurements have been performed with a fluorescence microscope Zei(beta) Axioplan, extended by a PMT and a CCD camera. During examination, the cell cultures were kept under nearly physiological conditions using a specialized perfusion chamber. The measurements took place on cellular monolayers. Different excitation geometries have been studied to overcome the difficulties, which arose from the very weak absorption of the cell monolayer, resulting in a low quantum yield and SNR. In classical cytotoxicity studies, only the statistical long-time effects (e.g. IC50) of cell damages are recorded. By redox microspectrofluorometry it is possible to observe the process of damage in its progress, shown by the presented results. In the second, more complex model, we studied the reaction of cells on ligands like PIA (Phenylisopropyladenosin). In this case, the intracellular reaction is connected with an increased production of cAMP. Again, this requires an increased production of ATP, which leads to an activation of the cellular energy metabolism. The spectroscopic results are interpreted by a first model.

  12. Comparative Study on Sino-US Campus Culture

    Institute of Scientific and Technical Information of China (English)

    苏二梅

    2015-01-01

    Cultural communication is playing an increasingly important role in the communication between between China and the United States,which not only promotes the communication and cooperation between the two countries,but provides experience and reference for the development of campus culture and education.We should find the root causes,draw lessons from the essences of American campus culture based on our excellent traditional culture,create campus culture with our own national characteristics to train comprehensive high-quality talents and develop the education system of our country.

  13. Electron microscopy methods in studies of cultural heritage sites

    Science.gov (United States)

    Vasiliev, A. L.; Kovalchuk, M. V.; Yatsishina, E. B.

    2016-11-01

    The history of the development and application of scanning electron microscopy (SEM), transmission electron microscopy (TEM), and energy-dispersive X-ray microanalysis (EDXMA) in studies of cultural heritage sites is considered. In fact, investigations based on these methods began when electron microscopes became a commercial product. Currently, these methods, being developed and improved, help solve many historical enigmas. To date, electron microscopy combined with microanalysis makes it possible to investigate any object, from parchment and wooden articles to pigments, tools, and objects of art. Studies by these methods have revealed that some articles were made by ancient masters using ancient "nanotechnologies"; hence, their comprehensive analysis calls for the latest achievements in the corresponding instrumental methods and sample preparation techniques.

  14. A PIXE study of vitrification of carnation in vitro culture

    Science.gov (United States)

    Yao, H. Y.; Lin, E. K.; Wang, C. W.; Yu, Y. C.; Chang, C. H.; Yang, Y. C.; Chang, C. Y.

    1996-04-01

    PIXE (Proton Induced X-ray Emission) is a well-known method for elemental analyses of specimens in applied studies. In this paper, we report results of an application of PIXE in trace-element analysis of normal and vitrified carnations in vitro culture. Experiments were performed to study the vitrification in connection with the trace elements in carnation tissues. About two hundred PIXE spectra were obtained from seventy samples with an irradiation of 3 MeV protons from the NEC 9SDH-2 Pelletron tandem accelerator. From the PIXE analysis we determined the trace element composition of normal and vitrified carnations. Our results indicate that there is a significant change of K, Ca, Fe and Zn contents in the vitrification process.

  15. Communicating Culture: An Exploratory Study of the Key Concepts in Maori Culture on Maori Web Sites

    Directory of Open Access Journals (Sweden)

    Zlatko J Kovacic

    2001-01-01

    Full Text Available We examine how accurately the belief system or cultural concepts of Maori, the indigenous people of New Zealand, is reconstructed in the virtual world of the Internet. Nine Maori web sites were searched using a list of 44 key concepts in Maori culture. We registered how many pages within a particular web site contain each of the key concepts. These numbers were set up in a data matrix for further statistical analysis. The Multidimensional Scaling method was used to construct a spatial representation of Maori web sites in the space generated by the key concepts in Maori culture. Using the correlation coefficients between derived dimensions and the key concepts we interpreted three dimensions as General Cultural, Intra-tribe Dynamics and Educational. The position of each Maori web site in this space has been located and described.

  16. The Changeable Nervous System: Studies On Neuroplasticity In Cerebellar Cultures

    Science.gov (United States)

    Seil, Fredrick J.

    2014-01-01

    Circuit reorganization after injury was studied in a cerebellar culture model. When cerebellar cultures derived from newborn mice were exposed at explantation to a preparation of cytosine arabinoside that destroyed granule cells and oligodendrocytes and compromised astrocytes, Purkinje cells surviving in greater than usual numbers were unensheathed by astrocytic processes and received twice the control number of inhibitory axosomatic synapses. Purkinje cell axon collaterals sprouted and many of their terminals formed heterotypical synapses with other Purkinje cell dendritic spines. The resulting circuit reorganization preserved inhibition in the cerebellar cortex. Following this reorganization, replacement of the missing granule cells and glia was followed by a restitution of the normal circuitry. Most of these developmental and reconstructive changes were not dependent on neuronal activity, the major exception being inhibitory synaptogenesis. The full complement of inhibitory synapses did not develop in the absence of neuronal activity, which could be mitigated by application of exogenous TrkB receptor ligands. Inhibitory synaptogenesis could also be promoted by activity-induced release of endogenous TrkB receptor ligands or by antibody activation of the TrkB receptor. PMID:24933693

  17. A Proteomic Study of Clavibacter Michiganensis Subsp. Michiganensis Culture Supernatants

    Directory of Open Access Journals (Sweden)

    Eva Hiery

    2015-11-01

    Full Text Available Clavibacter michiganensis, subsp. michiganensis is a Gram-positive plant pathogen infecting tomato (Solanum lycopersicum. Despite a considerable economic importance due to significant losses of infected plants and fruits, knowledge about virulence factors of C. michiganensis subsp. michiganensis and host-pathogen interactions on a molecular level are rather limited. In the study presented here, the proteome of culture supernatants from C. michiganensis subsp. michiganensis NCPPB382 was analyzed. In total, 1872 proteins were identified in M9 and 1766 proteins in xylem mimicking medium. Filtration of supernatants before protein precipitation reduced these to 1276 proteins in M9 and 976 proteins in the xylem mimicking medium culture filtrate. The results obtained indicate that C. michiganensis subsp. michiganensis reacts to a sucrose- and glucose-depleted medium similar to the xylem sap by utilizing amino acids and host cell polymers as well as their degradation products, mainly peptides, amino acids and various C5 and C6 sugars. Interestingly, the bacterium expresses the previously described virulence factors Pat-1 and CelA not exclusively after host cell contact in planta but already in M9 minimal and xylem mimicking medium.

  18. A Proteomic Study of Clavibacter Michiganensis Subsp. Michiganensis Culture Supernatants.

    Science.gov (United States)

    Hiery, Eva; Poetsch, Ansgar; Moosbauer, Tanja; Amin, Bushra; Hofmann, Jörg; Burkovski, Andreas

    2015-11-12

    Clavibacter michiganensis, subsp. michiganensis is a Gram-positive plant pathogen infecting tomato (Solanum lycopersicum). Despite a considerable economic importance due to significant losses of infected plants and fruits, knowledge about virulence factors of C. michiganensis subsp. michiganensis and host-pathogen interactions on a molecular level are rather limited. In the study presented here, the proteome of culture supernatants from C. michiganensis subsp. michiganensis NCPPB382 was analyzed. In total, 1872 proteins were identified in M9 and 1766 proteins in xylem mimicking medium. Filtration of supernatants before protein precipitation reduced these to 1276 proteins in M9 and 976 proteins in the xylem mimicking medium culture filtrate. The results obtained indicate that C. michiganensis subsp. michiganensis reacts to a sucrose- and glucose-depleted medium similar to the xylem sap by utilizing amino acids and host cell polymers as well as their degradation products, mainly peptides, amino acids and various C5 and C6 sugars. Interestingly, the bacterium expresses the previously described virulence factors Pat-1 and CelA not exclusively after host cell contact in planta but already in M9 minimal and xylem mimicking medium.

  19. VOLUNTEERING FOR CULTURAL HERITAGE CONSERVATION - TWO CASE STUDIES

    Directory of Open Access Journals (Sweden)

    Anca Maria VARODI

    2015-12-01

    Full Text Available A voluntary action is the key for individual benefits in addition to social ones. The voluntary actions for cultural heritage conservation are meant to provide gains for longs terms and to raise public awareness on the importance of conservation-restoration activities. During the past 5 years different volunteering actions for cultural heritage conservation organized by our faculty alone or in cooperation with the ASTRA Museum from Sibiu were finalized with exhibitions and public events. The case study presented in this paper is referring to the conservation-restoration of two long benches with backrest, dating from the first half of XXth century. The benches restored make parts from a group of artefact that was restored in the restoration camps at ASTRA Museum in 2013 and 2014. The particularity of the benches was de finishing layer that imitates the wood texture known as flander technique. This type of finishing is common to the landler community who was deported from Austria to Transylvania at the end of 17th century. The approach of conservations – restorations process was different for the artistic painting parts and needs special and appropriate intervention and investigation. While minimum interventions were making in order to preserve the objects for the future, we want to ensure that the value of less known technique can be well understood by younger generations.

  20. Brazilian Culture and Relationship Marketing: An Ethnographic Study in Retail

    Directory of Open Access Journals (Sweden)

    Cléria Donizete da Silva Lourenço

    2014-11-01

    Full Text Available This study was conducted in order to understand how Brazilian cultural traits are manifested in the relations of trade between employees of micro and small retailers and their customers. How, in marketing, one of the theoretical perspectives that seek to account for the relationship between businesses and consumers is relationship marketing, we sought to understand the exchanges and also to support this view. Assuming that the exchange ratio is also a commercial and social human relationship, we tried to treat the exchange as a social phenomenon. The research, qualitative in nature, was conducted in five retailers in a small city in southern Minas Gerais during the period of one year (Dec/2008 to Dec/2009 using ethnography as a method. As to results, as evidenced personalism, the warmth, the valuation of non-manual work, aversion to conflict, and the cunning knack manifested in trade relations. The main conclusion reached is that the reality of retail trade is complex and multifaceted as much as is the Brazilian culture