WorldWideScience

Sample records for public notice-form manner

  1. 40 CFR 141.203 - Tier 2 Public Notice-Form, manner, and frequency of notice.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 22 2010-07-01 2010-07-01 false Tier 2 Public Notice-Form, manner, and frequency of notice. 141.203 Section 141.203 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY...., house renters, apartment dwellers, university students, nursing home patients, prison inmates, etc...

  2. 40 CFR 141.204 - Tier 3 Public Notice-Form, manner, and frequency of notice.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 22 2010-07-01 2010-07-01 false Tier 3 Public Notice-Form, manner, and frequency of notice. 141.204 Section 141.204 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY...., house renters, apartment dwellers, university students, nursing home patients, prison inmates, etc...

  3. Report: EPA Needs Policies and Procedures to Manage Public Pesticide Petitions in a Transparent and Efficient Manner

    Science.gov (United States)

    Report #16-P-0019, October 27, 2015. OPP’s lack of policies and procedures to manage public pesticide petitions in a transparent and efficient manner can result in unreasonable delay lawsuits costing the agency time and resources.

  4. Mind Your Manners

    Energy Technology Data Exchange (ETDEWEB)

    Wiley, H. S.

    2010-01-01

    There has been a lot of talk in the media about the loss of courtesy in modern society. By many criteria, it seems that people in general have lost a degree of politeness. Reading some of the online comments after several recent articles in The Scientist would seem to indicate that biologists have also lost their manners. This lack of basic manners alarms me not only because of the obvious danger to our sense of community, but also because this type of behavior could damage society’s positive perception of scientists. Every time scientists (or anonymous bloggers posing as scientists) rant about the idiocy of someone who disagrees with them, our collective credibility erodes. Yes, there are a number of issues that we as scientists should be passionate about, but our objectives are best served by retaining an objective demeanor and respecting those to whom we happen to disagree.

  5. Speech Mannerisms: Games Clients Play

    Science.gov (United States)

    Morgan, Lewis B.

    1978-01-01

    This article focuses on speech mannerisms often employed by clients in a helping relationship. Eight mannerisms are presented and discussed, as well as possible interpretations. Suggestions are given to help counselors respond to them. (Author)

  6. Buried Messages, Hidden Meanings: Speech Mannerisms Revisited.

    Science.gov (United States)

    Morgan, Lewis B.

    1988-01-01

    Introduces counselors to 10 commonly used mannerisms of speech and the part that each mannerism plays in the communication process, especially in the counseling context. Offers suggestions on how to respond to these speech mannerisms in a straightforward and effective manner. (Author)

  7. On the Social Manners of Civil Servants before the Public Supervision%从新闻舆论监督的现实困境看公务人员的媒介素养

    Institute of Scientific and Technical Information of China (English)

    刘阳

    2014-01-01

    随着我国新闻舆论监督的作用和地位逐渐上升,公务人员面对新闻媒体履行职责的机会日渐增多。面对新闻媒体,公务人员以什么样的身份、形象、姿态呈现,不仅规约着行政职责的履行,同时也影响着公务机构的形象。因此,如何提高公务人员的媒介素养,就成为了当代公务机构建设的基本任务。%with the role and position of the news supervision of public opinion in our country gradually rise,civil servants face of the me-dia to perform the duties of the chance of growing. The face of the media,official show with what kind of identity,image,attitude,not only influence the performance of the administrative duties, but also affects the image of public institutions. Therefore, how to improve the civil servants' media literacy,became the basic task of contemporary public service institution building.

  8. Kant, Xunzi and the Artificiality of Manners

    Directory of Open Access Journals (Sweden)

    Anja BERNINGER

    2017-01-01

    Full Text Available Both Chinese and Western philosophers have argued for the ethical importance of manners. Their approaches are sometimes criticized on the grounds that manners are artificial. I compare Xunzi’s and Kant’s responses to this claim, and discuss the relevance of both positions for the development of a theory of manners. I show that there is no single artificiality claim, but rather four different claims: the claim that polite behavior lacks spontaneity, the claim that it is insincere, the claim that it goes against human nature, and the claim that it is arbitrary. While Kant is mainly concerned with the insincerity claim, Xunzi focusses on the claim that manners are arbitrary rules. Because of their different understandings of the function of manners both authors only provide a partial answer to the artificiality claim. To arrive at a full account of manners both perspectives must be combined.

  9. 45 CFR 1621.4 - Complaints by clients about manner or quality of legal assistance.

    Science.gov (United States)

    2010-10-01

    ... 45 Public Welfare 4 2010-10-01 2010-10-01 false Complaints by clients about manner or quality of...) LEGAL SERVICES CORPORATION CLIENT GRIEVANCE PROCEDURES § 1621.4 Complaints by clients about manner or... clients about the manner or quality of legal assistance that has been rendered by the recipient to the...

  10. The Generating Mechanism of the Perlocutionary Effects of Train Manner Posters

    OpenAIRE

    水田, 洋子

    2013-01-01

    Manner posters, which are used for the purpose of improving people’s manners in public spaces, attempt to be effective without being impositional. How do they meet the challenge? Manner posters can be considered to perform speech acts with written words and visual devices. The current work investigates the generating mechanism of their perlocutionary effects such as persuasion. It provides a case study of Japanese train manner posters by Tokyo Metro in 2008-2010. The analysis is conducted wit...

  11. 24 CFR 888.202 - Manner of publication.

    Science.gov (United States)

    2010-04-01

    ... 202 SUPPORTIVE HOUSING FOR THE ELDERLY PROGRAM AND SECTION 811 SUPPORTIVE HOUSING FOR PERSONS WITH DISABILITIES PROGRAM) SECTION 8 HOUSING ASSISTANCE PAYMENTS PROGRAM-FAIR MARKET RENTS AND CONTRACT RENT ANNUAL... published as market conditions indicate. In the case of revised factors applicable only to specific areas...

  12. Aplikasi Pembelajaran Table Manners Berbasis Multimedia

    OpenAIRE

    Yosanny, Agustinna; Pradipta, Albert; Viles, Dody; Pensen, Pensen

    2011-01-01

    Table manners adalah aturan-aturan pokok yang berlaku di meja makan. Aturan ini biasanya diterapkan padajamuan makan resmi. Penelitian ini bertujuan untuk merancang dan mengembangkan suatu aplikasipembelajaran tentang table manners untuk memudahkan dalam mempelajari aturan-aturan yang perlu diketahuidalam jamuan makan resmi, meliputi etika sebelum dan saat proses menyantap makanan, serta penggunaanalat-alat makan. Metode penelitian yang digunakan adalah metode Interactive Multimedia System De...

  13. Publicity.

    Science.gov (United States)

    Chisholm, Joan

    Publicity for preschool cooperatives is described. Publicity helps produce financial support for preschool cooperatives. It may take the form of posters, brochures, newsletters, open house, newspaper coverage, and radio and television. Word of mouth and general good will in the community are the best avenues of publicity that a cooperative nursery…

  14. SOEKARNO: HIS MANNERISM AND METHOD OF COMMUNICATION

    OpenAIRE

    Justin Wejak

    2000-01-01

    The main purpose of this article is to discuss Soekarno's mannerism and method of communication. Certain aspects such as Soekarno's use of language in his speeches are highlighted here in order to provide some basic understanding of Soekarno - both as a person and a political leader of the nation. The article aims at stimulating further discussions concerning this very well known leader. This article also examines Soeharto's style of speech for a comparison.

  15. SOEKARNO: HIS MANNERISM AND METHOD OF COMMUNICATION

    Directory of Open Access Journals (Sweden)

    Justin Wejak

    2000-01-01

    Full Text Available The main purpose of this article is to discuss Soekarno's mannerism and method of communication. Certain aspects such as Soekarno's use of language in his speeches are highlighted here in order to provide some basic understanding of Soekarno - both as a person and a political leader of the nation. The article aims at stimulating further discussions concerning this very well known leader. This article also examines Soeharto's style of speech for a comparison.

  16. Going “old school”: From bedside manner to deskside manner

    Directory of Open Access Journals (Sweden)

    David Fleischman

    2015-08-01

    Full Text Available Interaction between tertiary educators and students, we contend, improves trust and betters student responses to emotional distress while at university. Therefore, we introduce the “Deskside Manner Framework” as an emerging practice in the tertiary teaching and learning context to aid student transition success. Based on concepts and practice that originated primarily in the medical profession and later in other high credence professional contexts, the deskside manner framework includes: show respect, critical listening, the four Bs and follow up. Deskside manner is transferrable and we aim to facilitate it through workshops and by developing a digital repository of educator-student interaction stories.  

  17. Institutional traditions in teachers' manners of teaching

    Science.gov (United States)

    Lundqvist, Eva; Almqvist, Jonas; Östman, Leif

    2012-03-01

    The aim of this article is to make a close case study of one teacher's teaching in relation to established traditions within science education in Sweden. The teacher's manner of teaching is analysed with the help of an epistemological move analysis. The moves made by the teacher are then compared in a context of educational philosophy and selective tradition. In the analyses the focus is to study the process of teaching and learning in action in institutionalised and socially shared practices. The empirical material consists of video recordings of four lessons with the same group of students and the same teacher. The students are all in Year 7 in a Swedish 9-year compulsory school. During these lessons the students work with a subject area called "Properties of materials". The results show that the teacher makes a number of different moves with regard to how to proceed and come to a conclusion about what the substances are. Many of these moves are special in that they indicate that the students need to be able to handle the procedural level of school science. These moves do not deal directly with the knowledge production process, but with methodological aspects. The function of the moves turns the students' attention from one source of knowledge to another. The moves are aimed at helping the students to help themselves, since it is through their own activity and their own thinking that learning takes place. This is characteristic in the teacher's manner of teaching. When compared in a context of educational philosophy, this manner of teaching has similarities with progressentialism; a mixture of essentialism and progressivism. This educational philosophy is a central aspect of what is called the academic tradition—a selective tradition common in science education in Sweden between 1960 and 1990.

  18. Do children with obesity have worse table manners? Associations between child table manners, weight status and weight gain.

    Science.gov (United States)

    Briones, Naomi F; Cesaro, Robert J; Appugliese, Danielle P; Miller, Alison L; Rosenblum, Katherine L; Pesch, Megan H

    2018-06-01

    Children with obesity experience stigma stemming from stereotypes, one such stereotype is that people with obesity are "sloppy" or have poor manners. Teaching children "proper table manners" has been proposed as an obesity prevention strategy. Little is known about the association between children's weight status and table manners. To examine correlates of child table manners and to examine the association of child table manners with child obese weight status and prospective change in child body mass index z-score (BMIz). Mother-child dyads (N = 228) participated in a videotaped laboratory eating task with cupcakes. Coding schemes to capture child table manners (making crumbs, chewing with mouth open, getting food on face, shoving food in mouth, slouching, and getting out of seat), and maternal attentiveness to child table manners, were reliably applied. Anthropometrics were measured at baseline and at follow-up two years later. Regression analyses examined the association of participant characteristics with child table manners, as well as the associations of child table manners with child obese weight status, and prospective change in BMIz/year. Predictors of poorer child table manners were younger child age, greater cupcake consumption, and greater maternal attentiveness to child table manners. Poorer child table manners were not associated with child obese (vs. not) weight status, but were associated with a prospective decrease in BMIz/year in children with overweight/obesity. Obesity interventions to improve table manners may be perpetuating unfavorable stereotypes and stigma. Future work investigating these associations is warranted to inform childhood obesity guidelines around table manners. Copyright © 2018 Elsevier Ltd. All rights reserved.

  19. 26 CFR 1.9005-4 - Manner of exercising election.

    Science.gov (United States)

    2010-04-01

    ... 26 Internal Revenue 13 2010-04-01 2010-04-01 false Manner of exercising election. 1.9005-4 Section 1.9005-4 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) INCOME TAX (CONTINUED) INCOME TAXES General Actuarial Valuations § 1.9005-4 Manner of exercising election. (a...

  20. 26 CFR 1.9004-4 - Manner of exercising election.

    Science.gov (United States)

    2010-04-01

    ... 26 Internal Revenue 13 2010-04-01 2010-04-01 false Manner of exercising election. 1.9004-4 Section 1.9004-4 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) INCOME TAX (CONTINUED) INCOME TAXES General Actuarial Valuations § 1.9004-4 Manner of exercising election. (a...

  1. 26 CFR 1.9002-8 - Manner of exercising elections.

    Science.gov (United States)

    2010-04-01

    ... 26 Internal Revenue 13 2010-04-01 2010-04-01 false Manner of exercising elections. 1.9002-8 Section 1.9002-8 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) INCOME TAX (CONTINUED) INCOME TAXES General Actuarial Valuations § 1.9002-8 Manner of exercising elections...

  2. 26 CFR 1.9003-4 - Manner of exercising election.

    Science.gov (United States)

    2010-04-01

    ... 26 Internal Revenue 13 2010-04-01 2010-04-01 false Manner of exercising election. 1.9003-4 Section 1.9003-4 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) INCOME TAX (CONTINUED) INCOME TAXES General Actuarial Valuations § 1.9003-4 Manner of exercising election. (a...

  3. 26 CFR 1.853-4 - Manner of making election.

    Science.gov (United States)

    2010-04-01

    ... 26 Internal Revenue 9 2010-04-01 2010-04-01 false Manner of making election. 1.853-4 Section 1.853-4 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) INCOME TAX (CONTINUED) INCOME TAXES Regulated Investment Companies and Real Estate Investment Trusts § 1.853-4 Manner of...

  4. Mannerisms of the Elderly and Approaches to Rapport.

    Science.gov (United States)

    French, Warren A.; And Others

    1980-01-01

    Many older adults react to old age with empirically identified distinguishable mannerisms. Using these mannerisms, or behavior patterns, as a basis for rapport, gerontologists can: (1) uncover causes of adjustment problems; (2) assess seriousness of problems; and (3) implement counseling suggestions. (Author/JAC)

  5. 29 CFR 1905.21 - Manner of service.

    Science.gov (United States)

    2010-07-01

    ...-STEIGER OCCUPATIONAL SAFETY AND HEALTH ACT OF 1970 Hearings § 1905.21 Manner of service. Service of any... 29 Labor 5 2010-07-01 2010-07-01 false Manner of service. 1905.21 Section 1905.21 Labor Regulations Relating to Labor (Continued) OCCUPATIONAL SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR...

  6. Improving the livelihoods of wool producers in a sustainable manner ...

    African Journals Online (AJOL)

    Improving the livelihoods of wool producers in a sustainable manner by optimizing the woolled sheep production systems within the communal farming area of the Eastern Cape. “A vision that is future directed”

  7. The Manners of Liberalism: A Question of Limits.

    Science.gov (United States)

    Allen, William B.

    1982-01-01

    The argument that liberal education leads people to substitute intellectual tolerance or ambiguity for righteous indignation is criticized, with reference to Harriet Beecher Stowe's portrayal of Thomas Jefferson, his liberalism, and democratic manners. (MSE)

  8. 29 CFR 1614.703 - Manner and format of data.

    Science.gov (United States)

    2010-07-01

    ... § 1614.704(a) through (m). (3) When posting data pursuant to § 1614.704(d) and (j), bases of... 29 Labor 4 2010-07-01 2010-07-01 false Manner and format of data. 1614.703 Section 1614.703 Labor... Retaliation Act of 2002 (No FEAR Act) § 1614.703 Manner and format of data. (a) Agencies shall post their...

  9. Mind your "smoking manners": the tobacco industry tactics to normalize smoking in Japan.

    Science.gov (United States)

    Kashiwabara, Mina; Armada, Francisco

    2013-11-09

    The tobacco industry has adapted its promotional strategies as tobacco-control measures have increased. This paper describes the tobacco industry's strategies on smoking manners and illustrates how these interfere with tobacco-control policy in Japan where tobacco control remains weak. Information on the tobacco industry's promotional strategies in Japan was collected through direct observation, a review of tobacco industry documents and a literature review. The limitation of the study would be a lack of industry documents from Japan as we relied on a database of a U.S. institution to collect internal documents from the tobacco industry. Japan Tobacco began using the manners strategies in the early 1960s. Collaborating with wide range of actors -including local governments and companies- the tobacco industry has promoted smoking manners to wider audiences through its advertising and corporate social responsibility activities. The tobacco industry in Japan has taken advantage of the cultural value placed on manners in Japan to increase the social acceptability of smoking, eventually aiming to diminish public support for smoke-free policies that threatens the industry's business. A stronger enforcement of the WHO Framework Convention on Tobacco Control is critical to counteracting such strategies.

  10. 21 CFR 1306.05 - Manner of issuance of prescriptions.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 9 2010-04-01 2010-04-01 false Manner of issuance of prescriptions. 1306.05 Section 1306.05 Food and Drugs DRUG ENFORCEMENT ADMINISTRATION, DEPARTMENT OF JUSTICE PRESCRIPTIONS... revised, effective June 1, 2010. For the convenience of the user, therevised text is set forth as follows...

  11. Grand Manner Aesthetics in Landscape: From Canvas to Celluloid

    Science.gov (United States)

    Auger, Emily E.

    2009-01-01

    The methods by which environmental issues are aestheticized in late-twentieth-century film is directly and historically related to those established for grand manner painters by Nicholas Poussin (1594-1665) and taught at the French academy from the seventeenth through the nineteenth centuries. That these fundamentals were part of the training of…

  12. Stereotypes: A Preliminary Report on Mannerisms and Blindisms.

    Science.gov (United States)

    Leonhardt, M.

    1990-01-01

    The article describes current research on stereotypes in a group of blind children in Barcelona, Spain. Mannerisms and blindisms covering a range of verbal and motor behavior are defined and discussed, as are the variables of behavior related to them. (Author/DB)

  13. 26 CFR 1.1071-4 - Manner of election.

    Science.gov (United States)

    2010-04-01

    ... corporation, the statement shall be signed with the corporate name, followed by the signature and title of an officer of the corporation empowered to sign for the corporation, and the corporate seal must be affixed...) INCOME TAXES Changes to Effectuate F.c.c. Policy § 1.1071-4 Manner of election. (a) An election under the...

  14. 20 CFR 335.2 - Manner of claiming sickness benefits.

    Science.gov (United States)

    2010-04-01

    ..., or in the case of a female employee, pregnancy, miscarriage, or childbirth, an employee must file the... 20 Employees' Benefits 1 2010-04-01 2010-04-01 false Manner of claiming sickness benefits. 335.2 Section 335.2 Employees' Benefits RAILROAD RETIREMENT BOARD REGULATIONS UNDER THE RAILROAD UNEMPLOYMENT...

  15. 18 CFR 344.2 - Manner of submitting quotations.

    Science.gov (United States)

    2010-04-01

    ... quotations. 344.2 Section 344.2 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY REGULATIONS UNDER THE INTERSTATE COMMERCE ACT FILING QUOTATIONS FOR U.S. GOVERNMENT SHIPMENTS AT REDUCED RATES § 344.2 Manner of submitting quotations. (a) The quotation or tender...

  16. NARRATIVE PERSPECTIVE MEDIATED BY MANNER OF MOTION VERBS

    Directory of Open Access Journals (Sweden)

    Olesea BODEAN-VOZIAN

    2016-12-01

    Full Text Available There is typological variation in the way languages encode manner as an element of a motion event. Languages like English view it as relevant, and the lexicalization of the variety of ways to move results in a rich class of motion verbs, contrary to other types of languages, like Romanian, which leave the manner element to be encoded by verbids or adverbs (for these reasons some linguists refer to the first type as manner-rich and second type as manner-poor languages. Still, several studies contrasting typologically different languages showed that languages of the latter type are not so poor in manner-of-motion verbs. The question then might rather be: which manner components are more likely to be lexicalized?For research purposes, we distinguish manner in terms of objective elements (medium, speed or intensity and subjective elements (attitude, intention. The aim of the study is to focus on the manner-of-motion verbs that embed an evaluative or qualitative dimension of motion and to examine the way these verbs encode somebody’s perspective in a narrative. The first question in such a case is whose evaluation or point of view is being represented. The second one is how the subjective point of view (narrative perspective mediated through manner-of-motion verbs in an English narrative (The Lord of the Rings, by J.R.R. Tolkien is translated into Romanian, supposedly a manner-poor or low-manner language.PERSPECTIVA NARATIVĂ MEDIATĂ DE VERBELE DE MIŞCARE DE MODExistă o variaţie tipologică în felul în care limbile codifică modul ca element al unui eveniment de mişcare. Limbile precum engleza îl percep drept unul relevant, iar lexicalizarea gamei de mijloace de redare a mişcării a dat naştere unei clase bogate de verbe de mişcare, contrar altor tipuri de limbi, aşa ca româna, în care elementul ce redă modul este codificat de gerunziu sau adverbe. Din aceasta cauză, unii lingvişti numesc primul tip limbi bogate în verbe de mod (

  17. Practicing leadership-as-practice in content and manner

    OpenAIRE

    Raelin, Joe; Kempster, Stephen John; Youngs, Howard; Caroll, Brigid; Jackson, Brad

    2018-01-01

    A collective and collaborative response to an article appearing in Leadership’s “Leading Questions” department is prepared by a team subscribing to the leadership-as-practice approach. The focus is to represent the manner in which leadership-as-practice operates as a leadership theory and in its communal practice orientation. Among the themes addressed are leadership-as-practice’s theory development, its contribution in comparison to critical leadership theory, its approach to power, and its ...

  18. MANNER OF STOCKS SORTING USING CLUSTER ANALYSIS METHODS

    Directory of Open Access Journals (Sweden)

    Jana Halčinová

    2014-06-01

    Full Text Available The aim of the present article is to show the possibility of using the methods of cluster analysis in classification of stocks of finished products. Cluster analysis creates groups (clusters of finished products according to similarity in demand i.e. customer requirements for each product. Manner stocks sorting of finished products by clusters is described a practical example. The resultants clusters are incorporated into the draft layout of the distribution warehouse.

  19. Metal stressors consistently modulate bacterial conjugal plasmid uptake potential in a phylogenetically conserved manner

    DEFF Research Database (Denmark)

    Klümper, Uli; Dechesne, Arnaud; Riber, Leise

    2017-01-01

    The environmental stimulants and inhibitors of conjugal plasmid transfer in microbial communities are poorly understood. Specifically, it is not known whether exposure to stressors may cause a community to alter its plasmid uptake ability. We assessed whether metals (Cu, Cd, Ni, Zn) and one metal...... that community permissiveness is sensitive to metal(loid) stress in a manner that is both partially consistent across stressors and phylogenetically conserved.The ISME Journal advance online publication, 2 August 2016; doi:10.1038/ismej.2016.98....

  20. 24 CFR 888.115 - Fair market rents for existing housing: Manner of publication.

    Science.gov (United States)

    2010-04-01

    ... 24 Housing and Urban Development 4 2010-04-01 2010-04-01 false Fair market rents for existing..., SECTION 202 DIRECT LOAN PROGRAM, SECTION 202 SUPPORTIVE HOUSING FOR THE ELDERLY PROGRAM AND SECTION 811... MARKET RENTS AND CONTRACT RENT ANNUAL ADJUSTMENT FACTORS Fair Market Rents § 888.115 Fair market rents...

  1. Silence and table manners: when environments activate norms.

    Science.gov (United States)

    Joly, Janneke F; Stapel, Diederik A; Lindenberg, Siegwart M

    2008-08-01

    Two studies tested the conditions under which an environment (e.g., library, restaurant) raises the relevance of environment-specific social norms (e.g., being quiet, using table manners). As hypothesized, the relevance of such norms is raised when environments are goal relevant ("I am going there later") and when they are humanized with people or the remnants of their presence (e.g., a glass of wine on a table). Two studies show that goal-relevant environments and humanized environments raise the perceived importance of norms (Study 1) and the intention to conform to norms (Study 2). Interestingly, in both studies, these effects reach beyond norms related to the environments used in the studies.

  2. Influence of manner of heating on functioning of fireplace

    International Nuclear Information System (INIS)

    Kouki, J.

    1994-01-01

    In connection with a bioenergy research programme, there is an ongoing project for developing of wood-fired fireplaces. The overall target in the project is through research and development to improve the combustion and heating characteristics of fireplaces so that their harmful flue gas emissions are reduced and they become more appropriate sources of supplementary and reserve energy (e.g. when installed in electrically heated houses). In connection with this project, TTS Institute's Forestry Department conducted a study on the heating properties of a lightweight iron stove. Correct manner of heating is a means to reducing the amount of harmful flue gases produced by fireplaces and of raising their combustion efficiency. The content of environmentally harmful gases in the flue gases was at Its maximum when the stove was operated with little draught and with the combustion chamber full of fuel. By increasing the draught from 4 Pa to 32 Pa, the carbon monoxide content of the non-combusted gases was reduced almost by 75 percent. This was accompanied by nearly a doubling of the free heat loss of the flue gases. As well as being influenced by the draught in the flue, the functioning of the stove and completeness of combustion was influenced by the moisture content of the chopped firewood. A rise in the moisture level from 13 % to 40 % led to nearly a fivefold increase in the proportion of combustible gases in the flue gases

  3. [Perception manner of mother of obese persons in adolescence].

    Science.gov (United States)

    Radoszewska, Joanna

    2007-01-01

    There are specific relation properties of mother to the obese child. The mother representation in child is understand as a experience manner of herself. Representation (mental image) include perceptions, knowledge about characteristics and behaviors of a person and evaluation and experiences that provoke in others. The aim of this article is a trial of an answer what is a mental representation of mother experienced by obese girls and boys in adolescence. 21 obese persons (12 girls and 9 boys) and 23 persons of normal body mass (15 girls and 8 boys) have been investigated. The mean age of the investigated obese persons were 14.53, and for a person of normal body mass was 15.31. All persons were investigated by a clinical interview with 6 questions concerning mental mother representation. The obtained results were analyzed in relation to mental mother representation contents: cognitive, emotional, social, sexual, certificate, behavioral and somatic. Obese persons more often than the person of the normal body mass identify to mental mother representation somatic contents, more rarely social, sexual and behavioral contents. Obese girls more rarely than girls of the normal body mass identify to social contents, more often to somatic. Obese girls more rarely than obese boys identify to mental mother representation emotional contents, more often to somatic contents. The specific relation properties of mother to obese person in adolescence could be manifested in difficulties in turn on contents of mental mother representation, in external, somatic point of concern for mental mother representation, difficulties in contact with mental contents of mother representation.

  4. Public Values and Public Service Motivation

    DEFF Research Database (Denmark)

    Andersen, Lotte Bøgh; Beck Jørgensen, Torben; Kjeldsen, Anne Mette

    2013-01-01

    The literatures on public values (PV) and public service motivation (PSM) both address whether public service delivery is driven by something more than self-interest. They have developed separately, although they can benefit from insights developed by the other, and this article discusses...... conceptual and empirical relationships between them. Based on a survey of 501 public managers, we find that PSM and PV are associated empirically, but not in a manner allowing total integration. The conceptual discussion reveals how the two concepts cannot be totally separated, as values can be motivating...

  5. 48 CFR 1371.101 - Inspection and manner of doing work.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Inspection and manner of... DEPARTMENT SUPPLEMENTAL REGULATIONS ACQUISITIONS INVOLVING SHIP CONSTRUCTION AND SHIP REPAIR Provisions and Clauses 1371.101 Inspection and manner of doing work. Insert clause 1352.271-70, Inspection and Manner of...

  6. 26 CFR 1.1044(a)-1 - Time and manner for making election under the Omnibus Budget Reconciliation Act of 1993.

    Science.gov (United States)

    2010-04-01

    ... Nontaxable Exchanges § 1.1044(a)-1 Time and manner for making election under the Omnibus Budget... sale if the taxpayer purchases common stock or a partnership interest in a specialized small business investment company (SSBIC) within the 60-day period beginning on the date the publicly traded securities are...

  7. Publicity and public relations

    Science.gov (United States)

    Fosha, Charles E.

    1990-01-01

    This paper addresses approaches to using publicity and public relations to meet the goals of the NASA Space Grant College. Methods universities and colleges can use to publicize space activities are presented.

  8. Unacknowledged threats proffered "in a manner of speaking": recognizing workplace bullying as shaming.

    Science.gov (United States)

    Dzurec, Laura Cox; Kennison, Monica; Albataineh, Raya

    2014-07-01

    The purpose of this study was to examine workplace bullying victims' perceptions of what they heard their bully counterparts say through their use of prosody. From a sampling frame of 89 manuscripts referenced in the authors' previous studies, we identified a subset (n = 10) that included quotes regarding bullying victims' perceptions of communication experiences with their bully perpetrators. We used hermeneutics and a recursive metasynthesis to interpret quotes embedded in the manuscripts chosen for this study. Two-thirds of language is expressed nonverbally through prosody or "manner of speaking"-rhythm, stress, intonation, and vocabulary choice. We found that as bullies communicated with their intended victims over time, they used prosody across subtle, linked communications, or boldly and openly in public venues, to establish a context-embedded, one-way communication process of "doublespeak." Bullies' confusing prosodic communication processes served to recontexualize victims' situations and, through mechanisms largely unacknowledged by the victims, to subtly demean their personhood, and to shame them and render them voiceless. This study directs formal attention to the language of workplace bullying. Further study might strengthen opportunities to effectively address and curtail the long-term personal, professional, and organizational injuries deriving from workplace bullying. © 2014 Sigma Theta Tau International.

  9. 26 CFR 1.669(b)-2 - Manner of exercising election.

    Science.gov (United States)

    2010-04-01

    ... 26 Internal Revenue 8 2010-04-01 2010-04-01 false Manner of exercising election. 1.669(b)-2 Section 1.669(b)-2 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED... Years Beginning Before January 1, 1969 § 1.669(b)-2 Manner of exercising election. (a) By whom election...

  10. 26 CFR 301.6316-5 - Manner of paying tax by foreign currency.

    Science.gov (United States)

    2010-04-01

    ... currency to be deposited shall be that amount which, when converted at the rate of exchange used on the... 26 Internal Revenue 18 2010-04-01 2010-04-01 false Manner of paying tax by foreign currency. 301....6316-5 Manner of paying tax by foreign currency. (a) Time and place to pay. The unpaid tax required to...

  11. Point of View: Manner of Realising The Matter in Adichie's Half of A ...

    African Journals Online (AJOL)

    manner' to create meaning or matter. The best form of art is the blending of form and content; means and end; manner and matter. Therefore, the uniqueness of any novelist's product is contingent upon how well or otherwise their peculiar ...

  12. 26 CFR 1.871-1 - Classification and manner of taxing alien individuals.

    Science.gov (United States)

    2010-04-01

    ... 26 Internal Revenue 9 2010-04-01 2010-04-01 false Classification and manner of taxing alien... (CONTINUED) INCOME TAX (CONTINUED) INCOME TAXES Nonresident Aliens and Foreign Corporations § 1.871-1 Classification and manner of taxing alien individuals. (a) Classes of aliens. For purposes of the income tax...

  13. 26 CFR 1.522-2 - Manner of taxation of cooperative associations subject to section 522.

    Science.gov (United States)

    2010-04-01

    ... 26 Internal Revenue 7 2010-04-01 2010-04-01 true Manner of taxation of cooperative associations subject to section 522. 1.522-2 Section 1.522-2 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF...-2 Manner of taxation of cooperative associations subject to section 522. (a) In general. Farmers...

  14. Benford's Law for Quality Assurance of Manner of Death Counts in Small and Large Databases.

    Science.gov (United States)

    Daniels, Jeremy; Caetano, Samantha-Jo; Huyer, Dirk; Stephen, Andrew; Fernandes, John; Lytwyn, Alice; Hoppe, Fred M

    2017-09-01

    To assess if Benford's law, a mathematical law used for quality assurance in accounting, can be applied as a quality assurance measure for the manner of death determination. We examined a regional forensic pathology service's monthly manner of death counts (N = 2352) from 2011 to 2013, and provincial monthly and weekly death counts from 2009 to 2013 (N = 81,831). We tested whether each dataset's leading digit followed Benford's law via the chi-square test. For each database, we assessed whether number 1 was the most common leading digit. The manner of death counts first digit followed Benford's law in all the three datasets. Two of the three datasets had 1 as the most frequent leading digit. The manner of death data in this study showed qualities consistent with Benford's law. The law has potential as a quality assurance metric in the manner of death determination for both small and large databases. © 2017 American Academy of Forensic Sciences.

  15. Assessment of Ethical Ideals and Ethical Manners in Care of Older People

    OpenAIRE

    Frilund, Marianne; Fagerström, Lisbeth; Eriksson, Katie; Eklund, Patrik

    2013-01-01

    The aim of this study is to establish structured clusters and well-defined ontological entities (nodes) describing ethical values as both ideal and opportunity for ethical manner as perceived by the caregiver. In this study, we use Bayesian Belief Networks (BBNs) to analyse ethical values (ethos) and ethical manners in daily work with older people. Material is based on questionnaire data collected by the instrument for the self-assessment of individual ethos in the care of older people (ISAEC...

  16. Human, recombinant interleukin-2 induces in vitro histamine release in a dose-dependent manner

    DEFF Research Database (Denmark)

    Nielsen, Hans Jørgen; Petersen, L J; Skov, P S

    1995-01-01

    significantly in the supernatant from cells stimulated by rIL-2 in a dose-dependent manner both in patients and volunteers. Total cell-bound histamine was 49.3 +/- 4.1 ng/ml in patients compared to 78.5 +/- 7.7 ng/ml in volunteers (p ... was significantly enhanced in cancer patients compared to volunteers (*p manner in both cancer patients and volunteers. This may in part explain the severe toxicity observed during high...

  17. Pragmatic awareness of Suggestions: From (Im)Polite Mannerism to Attitudinal Appropriateness

    OpenAIRE

    Hamid Allami; Nasim Boustani

    2017-01-01

    The present research seeks to determine: (a) Iranian EFL learners’ application of suggestion semantic formulae ;(b) their attitude of appropriateness in terms of confidence in the employment of appropriate supportive moves; (c), their (im)polite mannerism with respect to the selected strategies; and (d) the relationship between attitude of appropriateness and mannerism of (im)politeness. An Oxford Quick placement Test (OQPT) was administered among 60 Iranian EFL learners to check their langua...

  18. Globalized conflicts, globalized responses. Changing manners of contestation among indigenous communities

    DEFF Research Database (Denmark)

    Benyei, Petra; Turreira Garcia, Nerea; Orta-Martínez, Martí

    2017-01-01

    In a globalized world, environmental conflicts affecting indigenous communities (including hunter-gatherer groups) have intensified and grown in their transnational character. These changes have affected the choice of manners of contestation of these groups, favouring in some cases the emergence...... activities and confront conflicts through a truly bottom-up approach. The chapter ends discussing how, despite the potential of such new manners of contestation, the power imbalances that currently underpin many indigenous conflicts are first to be addressed....

  19. A Mission to Reform Manners: Religion, Secularization, and Empire in Early Modern England

    OpenAIRE

    Taback, Naomi Johanna

    2013-01-01

    This dissertation looks at three voluntary societies formed in London shortly after the revolution of 1688. These societies, with overlapping membership, shared a broad vision for the improvement of manners and morals throughout the burgeoning British Empire. The Society for Reformation of Manners, founded in 1691, called for the enforcement of the civil laws against prostitution, swearing, drunkenness, gambling, and other moral crimes. The Society for Promoting Christian Knowledge, founde...

  20. Return on Investment in the Public Sector

    National Research Council Canada - National Science Library

    Bigham, Joshua

    2004-01-01

    .... Development and application of ROI analysis is challenging in the public sector since most government organizations do not generate profit necessary for calculation of ROI in the manner in which it...

  1. Pragmatic awareness of Suggestions: From (ImPolite Mannerism to Attitudinal Appropriateness

    Directory of Open Access Journals (Sweden)

    Hamid Allami

    2017-09-01

    Full Text Available The present research seeks to determine: (a Iranian EFL learners’ application of suggestion semantic formulae ;(b their attitude of appropriateness in terms of confidence in the employment of appropriate supportive moves; (c, their (impolite mannerism with respect to the selected strategies; and (d the relationship between attitude of appropriateness and mannerism of (impoliteness. An Oxford Quick placement Test (OQPT was administered among 60 Iranian EFL learners to check their language proficiency level and to satisfy the assumption of homogeneity among the learners. These participants along with 10 American native speakers then underwent a suggestion DCT. The questionnaire included questions addressing interlocutors’ with higher, lower or equal power status and intimate or strange social distance. The DCT also involved attitudinal appropriateness scale and (impoliteness mannerism likert scale to examine the learners’ degree of confidence and mannerism. Besides similarities and differences in the application of suggestion semantic formulae between the Iranian learners and American speakers, the results revealed variations in the two groups’ performances for appropriateness and (impoliteness. The results also indicated a positive relationship between the attitude and mannerism scales.

  2. The Reflection of Javanese Life Manner on the Dongkrek Art and Ritual Performance in Madiun Society

    Directory of Open Access Journals (Sweden)

    Sharfina Nur Amalina

    2018-05-01

    Full Text Available This article focuses in the analysis of Javanese life manner within Dongkrek art and ritual performance in Mejayan, Madiun, Indonesia. The purpose of research is to reflect a life manner of Javanese society within a Dongkrek show. The qualitative-descriptive was used as research approach. Data were collected by interview, literature review, and other relevant resources. The results of research show a five points of Javanese manner of life in Dongkrek, represent in the phrase Ambrasta Dur Hangkara, Memayu Hayuning Bawana, Sura Dira Jayaning Lebur Dening Pangastuti, Sadulur Papat Kalima Pancer, and Manunggaling Kawula Gusti. Those phrases contain many moral values that generally can be accepted by society. The phrase represents a human relationship, the manner of life, ways of life, world-view, willingness, and the transcendental relationship between humans and God. It means the purpose of the Javanese manner of life is to reach the perfection of life. The perfection of life is resting on the faith towards to the God through the maturity of spirituality.

  3. 26 CFR 1.881-1 - Manner of taxing foreign corporations.

    Science.gov (United States)

    2010-04-01

    ... tax avoidance purposes, whether or not such corporation is engaged in trade or business in the United... TAX (CONTINUED) INCOME TAXES Foreign Corporations § 1.881-1 Manner of taxing foreign corporations. (a) Classes of foreign corporations. For purposes of the income tax, foreign corporations are divided into two...

  4. 17 CFR 17.02 - Form, manner and time of filing reports.

    Science.gov (United States)

    2010-04-01

    ... 17 Commodity and Securities Exchanges 1 2010-04-01 2010-04-01 false Form, manner and time of filing reports. 17.02 Section 17.02 Commodity and Securities Exchanges COMMODITY FUTURES TRADING... markets located in that time zone, and central time for information concerning all other markets. (b...

  5. Organizing artistic activities in a recurrent manner : (on the nature of) entrepreneurship in the performing arts

    NARCIS (Netherlands)

    Bergamini, Michela; Van de Velde, Ward; Van Looy, Bart; Visscher, Klaasjan

    2017-01-01

    A majority of performing arts organizations active in classical music, theatre, and contemporary dance rely on funding from "third parties" in order to organize productions in a recurrent manner. We adopt an entrepreneurial perspective to inform the debate on the economic sustainability of

  6. Herp regulates Hrd1-mediated ubiquitylation in a ubiquitin-like domain-dependent manner

    DEFF Research Database (Denmark)

    Kny, Melanie; Standera, Sybille; Hartmann-Petersen, Rasmus

    2011-01-01

    in ER-associated protein degradation (ERAD) and interacts directly with the ubiquitin ligase Hrd1, which is found in high molecular mass complexes of the ER membrane. Here we present the first evidence that Herp regulates Hrd1-mediated ubiquitylation in a ubiquitin-like (UBL) domain-dependent manner. We...

  7. Impact of Video Feedback on Teachers' Eye-Contact Mannerisms in Microteaching.

    Science.gov (United States)

    Karasar, Niyazi

    To test the impact of video feedback on teachers' eye-contact mannerisms in microteaching in inservice vocational teacher education, the study utilized video recordings from the data bank generated by previous studies conducted at the Ohio State University's Center for Vocational and Technical Education. The tapes were assigned through a…

  8. Nucleoside analogues are activated by bacterial deoxyribonucleoside kinases in a species-specific manner

    DEFF Research Database (Denmark)

    Sandrini, Michael; Clausen, Anders; On, Stephen L. W.

    2007-01-01

    To investigate the bactericidal activity of antiviral and anticancer nucleoside analogues against a variety of pathogenic bacteria and characterize the activating enzymes, deoxyribonucleoside kinases (dNKs). Several FDA-approved nucleoside analogue drugs were screened for their potential bacteric......-specific manner. Therefore, nucleoside analogues have a potential to be employed as antibiotics in the fight against emerging multiresistant bacteria....

  9. Rheological properties of a nematic cell oriented in a planar manner

    International Nuclear Information System (INIS)

    Barbero, G.; Meyer, C.; Lelidis, I.

    2010-01-01

    We propose a simple model to investigate the rheological properties of a nematic cell oriented in a planar manner. The storage and loss modulus are evaluated in the case of strong and weak anchoring conditions. The contribution of the surface viscosity to the rheological parameters is also considered.

  10. Astrocytic connexin hemichannels are regulated by PKC phosphorylation in an isoform-specific manner

    DEFF Research Database (Denmark)

    MacAulay, N.; Alstrom, J. S.; Hansen, D. B.

    2017-01-01

    /activation of PKC and by mutational disruption of the proposed PKC-phosphorylation sites. Cx30 hemichannel activity, in contrast, was down-regulated by PKC activation, in a manner suggesting PKC-mediated channel closure. No single PKC consensus site could be assigned to this regulatory property by mutational...

  11. Weighting of Acoustic Cues to a Manner Distinction by Children with and without Hearing Loss

    Science.gov (United States)

    Nittrouer, Susan; Lowenstein, Joanna H.

    2015-01-01

    Purpose: Children must develop optimal perceptual weighting strategies for processing speech in their first language. Hearing loss can interfere with that development, especially if cochlear implants are required. The three goals of this study were to measure, for children with and without hearing loss: (a) cue weighting for a manner distinction,…

  12. Silence and table manners : When environments activate norms (Retracted article. See vol. 38, pg. 1378, 2012)

    NARCIS (Netherlands)

    Joly, Janneke F.; Stapel, Diederik A.; Lindenberg, Siegwart M.

    Two studies tested the conditions under which an environment (e. g., library, restaurant) raises the relevance of environment-specific social norms (e. g., being quiet, using table manners). As hypothesized, the relevance of such norms is raised when environments are goal relevant ("I am going there

  13. Non-natural manners of death among users of illicit drugs: Substance findings.

    Science.gov (United States)

    Delaveris, Gerd Jorunn M; Teige, Brita; Rogde, Sidsel

    2014-05-01

    The aim of the study was to explore differences and similarities between the various non-natural manners of death (accident, suicide, homicide) regarding toxicological findings in illicit drug users. Medicolegal autopsy reports from the Institute of Forensic Medicine University of Oslo concerning deaths from 2000 to 2009 were investigated. Those aged 20-59 whose manner of death was non-natural and who tested positive for any narcotic drug (morphine/heroin, amphetamines, ecstasy, cannabis, LSD, PCP, and high levels of GHB in addition to methadone and buprenorphine) were selected. All substance findings were registered and categorized (narcotics, ethanol, and medicinal products). Of the 1603 autopsies that met the selection criteria, 1204 were accidental intoxications, 122 accidents other than intoxication, 114 suicides by intoxication, 119 non-intoxication suicides, and 44 victims of homicide. Poly drug use was found in all manners of death. The drug profile as well as the mean number of substances (illicit drugs and medicinal products) varied from 2.9 to 4.6 substances per case, depending on the manner of death. Intoxication suicides had the highest number of substances and a total drug profile similar to accidental intoxications. Non-intoxication suicides had a total drug profile similar to homicide and accidents other than intoxication. The number of substances found per case increased during the decade, mainly due to increased findings of methadone, cannabis, amphetamines, and benzodiazepines. Methadone findings increased much more than buprenorphine. Methadone was found 20 times more often than buprenorphine in accidental intoxication cases. In summary, poly drug findings are common in adults who suffer a non-natural death while using illicit drugs. The different manners of death have some specific characteristics and significant differences regarding drug profile. Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

  14. Public Values

    DEFF Research Database (Denmark)

    Beck Jørgensen, Torben; Rutgers, Mark R.

    2015-01-01

    administration is approached in terms of processes guided or restricted by public values and as public value creating: public management and public policy-making are both concerned with establishing, following and realizing public values. To study public values a broad perspective is needed. The article suggest......This article provides the introduction to a symposium on contemporary public values research. It is argued that the contribution to this symposium represent a Public Values Perspective, distinct from other specific lines of research that also use public value as a core concept. Public...... a research agenda for this encompasing kind of public values research. Finally the contributions to the symposium are introduced....

  15. Considerations on the manner to account for fast fracture risk in the design of PWR vessels

    International Nuclear Information System (INIS)

    Pellisier-Tanon, A.; Grandemange, J.M.

    1985-08-01

    The way followed in France for analyzing fast fracture resistance of PWR primary components is the one of a deterministic analysis with safety coefficients imposed in the fracture criteria. The study of margins towards fast fracture of the 900 MWe program vessels undertaken in 1982 includes parametric evaluations of the influence of essential variables. It has stimulated further thoughts on the level of safety to fix in the analysis methodology, on the orientations for the choice of safety factors and on the manner to introduce them in the analysis. A first chapter tries to characterize the French approach in comparison to those of other countries. A second chapter examines the manner according to which safety factors can be introduced in the deterministic analysis. It presents the principle for a logical approach accounting for the interdependency of all factors and variables. It establishes criteria for the selection of defect kind and size for the computation

  16. 14 CFR 139.335 - Public protection.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Public protection. 139.335 Section 139.335... Operations § 139.335 Public protection. (a) In a manner authorized by the Administrator, each certificate... persons or vehicles; and (2) Reasonable protection of persons and property from aircraft blast. (b...

  17. Pig genome sequence - analysis and publication strategy

    DEFF Research Database (Denmark)

    Archibald, Alan L.; Bolund, Lars; Churcher, Carol

    2010-01-01

    preferentially selected for sequencing. In accordance with the Bermuda and Fort Lauderdale agreements and the more recent Toronto Statement the data have been released into public sequence repositories (Genbank/EMBL, NCBI/Ensembl trace repositories) in a timely manner and in advance of publication. CONCLUSIONS...

  18. p21-activated Kinase1(PAK1) can promote ERK activation in a kinase independent manner

    DEFF Research Database (Denmark)

    Wang, Zhipeng; Fu, Meng; Wang, Lifeng

    2013-01-01

    204) although phosphorylation of b-Raf (Ser445) and c-Raf (Ser 338) remained unchanged. Furthermore, increased activation of the PAK1 activator Rac1 induced the formation of a triple complex of Rac1, PAK1 and Mek1, independent of the kinase activity of PAK1. These data suggest that PAK1 can stimulate...... MEK activity in a kinase independent manner, probably by serving as a scaffold to facilitate interaction of c-Raf....

  19. Public lighting.

    NARCIS (Netherlands)

    Schreuder, D.A.

    1986-01-01

    The function of public lighting and the relationship between public lighting and accidents are considered briefly as aspects of effective countermeasures. Research needs and recent developments in installation and operational described. Public lighting is an efficient accident countermeasure, but

  20. Serotonin inputs to the dorsal BNST modulate anxiety in a 5-HT1A receptor-dependent manner.

    Science.gov (United States)

    Garcia-Garcia, A L; Canetta, S; Stujenske, J M; Burghardt, N S; Ansorge, M S; Dranovsky, A; Leonardo, E D

    2017-08-01

    Serotonin (5-HT) neurons project from the raphe nuclei throughout the brain where they act to maintain homeostasis. Here, we study 5-HT inputs into the bed nucleus of the stria terminalis (BNST), a major subdivision of the extended amygdala that has been proposed to regulate responses to anxiogenic environments in humans and rodents. While the dorsal part of the BNST (dBNST) receives dense 5-HT innervation, whether and how 5-HT in the dBNST normally modulates anxiety remains unclear. Using optogenetics, we demonstrate that activation of 5-HT terminals in the dBNST reduces anxiety in a highly anxiogenic environment. Further analysis revealed that optogenetic inhibition of 5-HT inputs into the dBNST increases anxiety in a less anxiogenic environment. We found that 5-HT predominantly hyperpolarizes dBNST neurons, reducing their activity in a manner that can be blocked by a 5-HT 1A antagonist. Finally, we demonstrate that activation of 5-HT 1A receptors in the dBNST is necessary for the anxiolytic effect observed following optogenetic stimulation of 5-HT inputs into the dBNST. These data reveal that 5-HT release in the dBNST modulates anxiety-like behavior via 5-HT 1A receptors under naturalistic conditions.Molecular Psychiatry advance online publication, 1 August 2017; doi:10.1038/mp.2017.165.

  1. The description of morality and manners in Vsevolod Solovyov’s short prose

    OpenAIRE

    Nikolsky Evgeny Vladimirovich

    2013-01-01

    This article contains a genre specificity of short prose by Vsevolod Solovyov, whose work is devoted to the Russian history between the 18th and 19th centuries. According to professor N.L. Vershinina, the author distinguishes the difference between the description of morality and manners. The main difference is in the presence of author’s point of view on the describing occurrences. In this case, such works are considered to be a kind of morality, which characterizes Solovyov’s works. Showing...

  2. The description of morality and manners in Vsevolod Solovyov’s short prose

    Directory of Open Access Journals (Sweden)

    Nikolsky Evgeny Vladimirovich

    2013-06-01

    Full Text Available This article contains a genre specificity of short prose by Vsevolod Solovyov, whose work is devoted to the Russian history between the 18th and 19th centuries. According to professor N.L. Vershinina, the author distinguishes the difference between the description of morality and manners. The main difference is in the presence of author’s point of view on the describing occurrences. In this case, such works are considered to be a kind of morality, which characterizes Solovyov’s works. Showing every-day life, he examined the process of turning barbarism into a civilizing society.

  3. Parental overprotection engenders dysfunctional attitudes about achievement and dependency in a gender-specific manner.

    Science.gov (United States)

    Otani, Koichi; Suzuki, Akihito; Matsumoto, Yoshihiko; Shibuya, Naoshi; Sadahiro, Ryoichi; Enokido, Masanori

    2013-12-24

    It has been suggested that dysfunctional attitudes, cognitive vulnerability to depression, have developmental origins. The present study examined the effects of parental rearing on dysfunctional attitudes in three areas of life with special attention to gender specificity. The subjects were 665 Japanese healthy volunteers. Dysfunctional attitudes were assessed by the 24-item Dysfunctional Attitude Scale, which has the Achievement, Dependency and Self-control subscales. Perceived parental rearing was assessed by the Parental Bonding Instrument, which has the Care and Protection subscales. Higher scores of the Achievement (β = 0.293, p overprotection engenders dysfunctional attitudes about achievement and dependency in a gender-specific manner.

  4. Tissue transglutaminase inhibits the TRPV5-dependent calcium transport in an N-glycosylation-dependent manner

    DEFF Research Database (Denmark)

    Boros, Sandor; Xi, Qi; Dimke, Henrik Anthony

    2011-01-01

    Tissue transglutaminase (tTG) is a multifunctional Ca(2+)-dependent enzyme, catalyzing protein crosslinking. The transient receptor potential vanilloid (TRPV) family of cation channels was recently shown to contribute to the regulation of TG activities in keratinocytes and hence skin barrier form......, these observations imply that tTG is a novel extracellular enzyme inhibiting the activity of TRPV5. The inhibition of TRPV5 occurs in an N-glycosylation-dependent manner, signifying a common final pathway by which distinct extracellular factors regulate channel activity....

  5. A Brief Analysis on The Mannerism of Strange Stories of A Chinese Studio by Pu Songling

    Directory of Open Access Journals (Sweden)

    Andyni Khosasih

    2012-10-01

    Full Text Available Strange Stories of a Chinese Studio, commonly known as 聊斋 (Liaozhai is a short novel written in classical Chinese from Qing dynasty. This novel was written in 1680, year 19 of Emperor Kangxi’s reign. The novel has 491 chapters. Article explored Pu Songling’s mannerism, such as the contents, material collection, innovation of artistic literary elements and images of women. It can be concluded that  the novel reflects the broadness of humanistic world and thoroughly describes images of women. It broke through the restriction of thoughts in feudalism society.  

  6. Public reactions to drone use in residential and public areas

    DEFF Research Database (Denmark)

    Bajde, Domen; Woerman, Niklas; Bruun, Maja Hojer

    The public will play a vital role in shaping the future of the drone sector. The sector’s fate is tied to factors such as the capacity to serve the public and convince it that drones can benefit society, the ability to ensure that drones are used in a safe and considerate manner, and the readiness...... and effectiveness of the sector to address public concerns, such as safety and privacy. This report addresses public reactions to drones in residential and public settings and the concerns they raised. We present the results of two studies conducted as part of a collaborative project between the University...... of Southern Denmark (SDU), Aalborg University (AAU) and the Danish Transport, Construction and Housing Authority. The report builds upon and supplements the research conducted in the initial phase of the project (Bajde et al. 2017)....

  7. TALE activators regulate gene expression in a position- and strand-dependent manner in mammalian cells.

    Science.gov (United States)

    Uhde-Stone, Claudia; Cheung, Edna; Lu, Biao

    2014-01-24

    Transcription activator-like effectors (TALEs) are a class of transcription factors that are readily programmable to regulate gene expression. Despite their growing popularity, little is known about binding site parameters that influence TALE-mediated gene activation in mammalian cells. We demonstrate that TALE activators modulate gene expression in mammalian cells in a position- and strand-dependent manner. To study the effects of binding site location, we engineered TALEs customized to recognize specific DNA sequences located in either the promoter or the transcribed region of reporter genes. We found that TALE activators robustly activated reporter genes when their binding sites were located within the promoter region. In contrast, TALE activators inhibited the expression of reporter genes when their binding sites were located on the sense strand of the transcribed region. Notably, this repression was independent of the effector domain utilized, suggesting a simple blockage mechanism. We conclude that TALE activators in mammalian cells regulate genes in a position- and strand-dependent manner that is substantially different from gene activation by native TALEs in plants. These findings have implications for optimizing the design of custom TALEs for genetic manipulation in mammalian cells. Copyright © 2013 Elsevier Inc. All rights reserved.

  8. Biochanin a and Daidzein Influence Meiotic Maturation of Pig Oocytes in a Different Manner

    Directory of Open Access Journals (Sweden)

    Hošková K.

    2014-09-01

    Full Text Available The aim of the study was to determine the influence of different concentrations of phytoestrogens biochanin A (BIO A; 20, 40, 50μg ml-1 and daidzein (DAI; 10, 20, 40, 50μg ml-1 on the course of meiotic maturation of pig oocytes. After a 24-hour cultivation, a stage of nuclear maturation was achieved and the areas of cumulus-oocyte complexes (COCs, as an indicator of cumulus expansion, were evaluated. The effects of both contaminants on oocytes were mani - fested from the lowest concentration used. Nuclear maturation was inhibited in a dose-dependent manner in the case of BIO A. Effects of DAI reached a plateau at a concentration of 20μg ml-1.Possible relationship to limited solubility of DAI was excluded because limits of DAI solubility in culture medium were confirmed at 50 μg ml-1.The cumulus expansion was also influenced in a different manner - reduction of the COC’s area by BIO A was dose-dependent, whereas DAI had the strongest effect on CCs in the lowest and highest concentrations used. Both phytoestrogens negatively influence the meiotic maturation of porcine oocytes but there are significant differences in their concrete effects which could relate to the diverse mechanisms of their acting on target cells.

  9. A top-down manner-based DCNN architecture for semantic image segmentation.

    Directory of Open Access Journals (Sweden)

    Kai Qiao

    Full Text Available Given their powerful feature representation for recognition, deep convolutional neural networks (DCNNs have been driving rapid advances in high-level computer vision tasks. However, their performance in semantic image segmentation is still not satisfactory. Based on the analysis of visual mechanism, we conclude that DCNNs in a bottom-up manner are not enough, because semantic image segmentation task requires not only recognition but also visual attention capability. In the study, superpixels containing visual attention information are introduced in a top-down manner, and an extensible architecture is proposed to improve the segmentation results of current DCNN-based methods. We employ the current state-of-the-art fully convolutional network (FCN and FCN with conditional random field (DeepLab-CRF as baselines to validate our architecture. Experimental results of the PASCAL VOC segmentation task qualitatively show that coarse edges and error segmentation results are well improved. We also quantitatively obtain about 2%-3% intersection over union (IOU accuracy improvement on the PASCAL VOC 2011 and 2012 test sets.

  10. L-carnosine affects the growth of Saccharomyces cerevisiae in a metabolism-dependent manner.

    Science.gov (United States)

    Cartwright, Stephanie P; Bill, Roslyn M; Hipkiss, Alan R

    2012-01-01

    The dipeptide L-carnosine (β-alanyl-L-histidine) has been described as enigmatic: it inhibits growth of cancer cells but delays senescence in cultured human fibroblasts and extends the lifespan of male fruit flies. In an attempt to understand these observations, the effects of L-carnosine on the model eukaryote, Saccharomyces cerevisiae, were examined on account of its unique metabolic properties; S. cerevisiae can respire aerobically, but like some tumor cells, it can also exhibit a metabolism in which aerobic respiration is down regulated. L-Carnosine exhibited both inhibitory and stimulatory effects on yeast cells, dependent upon the carbon source in the growth medium. When yeast cells were not reliant on oxidative phosphorylation for energy generation (e.g. when grown on a fermentable carbon source such as 2% glucose), 10-30 mM L-carnosine slowed growth rates in a dose-dependent manner and increased cell death by up to 17%. In contrast, in media containing a non-fermentable carbon source in which yeast are dependent on aerobic respiration (e.g. 2% glycerol), L-carnosine did not provoke cell death. This latter observation was confirmed in the respiratory yeast, Pichia pastoris. Moreover, when deletion strains in the yeast nutrient-sensing pathway were treated with L-carnosine, the cells showed resistance to its inhibitory effects. These findings suggest that L-carnosine affects cells in a metabolism-dependent manner and provide a rationale for its effects on different cell types.

  11. Probiotics and prebiotics: prospects for public health and nutritional recommendations

    NARCIS (Netherlands)

    Sanders, Mary Ellen; Lenoir-Wijnkoop, Irene; Salminen, Seppo; Merenstein, Daniel J.; Gibson, Glenn R.; Petschow, Bryon W.; Nieuwdorp, Max; Tancredi, Daniel J.; Cifelli, Christopher J.; Jacques, Paul; Pot, Bruno

    2014-01-01

    Probiotics and prebiotics are useful interventions for improving human health through direct or indirect effects on the colonizing microbiota. However, translation of these research findings into nutritional recommendations and public health policy endorsements has not been achieved in a manner

  12. Potentiation Effects of Half-Squats Performed in a Ballistic or Nonballistic Manner.

    Science.gov (United States)

    Suchomel, Timothy J; Sato, Kimitake; DeWeese, Brad H; Ebben, William P; Stone, Michael H

    2016-06-01

    This study examined and compared the acute effects of ballistic and nonballistic concentric-only half-squats (COHSs) on squat jump performance. Fifteen resistance-trained men performed a squat jump 2 minutes after a control protocol or 2 COHSs at 90% of their 1 repetition maximum (1RM) COHS performed in a ballistic or nonballistic manner. Jump height (JH), peak power (PP), and allometrically scaled peak power (PPa) were compared using three 3 × 2 repeated-measures analyses of variance. Statistically significant condition × time interaction effects existed for JH (p = 0.037), PP (p = 0.041), and PPa (p = 0.031). Post hoc analysis revealed that the ballistic condition produced statistically greater JH (p = 0.017 and p = 0.036), PP (p = 0.031 and p = 0.026), and PPa (p = 0.024 and p = 0.023) than the control and nonballistic conditions, respectively. Small effect sizes for JH, PP, and PPa existed during the ballistic condition (d = 0.28-0.44), whereas trivial effect sizes existed during the control (d = 0.0-0.18) and nonballistic (d = 0.0-0.17) conditions. Large statistically significant relationships existed between the JH potentiation response and the subject's relative back squat 1RM (r = 0.520; p = 0.047) and relative COHS 1RM (r = 0.569; p = 0.027) during the ballistic condition. In addition, large statistically significant relationship existed between JH potentiation response and the subject's relative back squat strength (r = 0.633; p = 0.011), whereas the moderate relationship with the subject's relative COHS strength trended toward significance (r = 0.483; p = 0.068). Ballistic COHS produced superior potentiation effects compared with COHS performed in a nonballistic manner. Relative strength may contribute to the elicited potentiation response after ballistic and nonballistic COHS.

  13. Intermittent Hypoxia Disrupts Glucose Homeostasis in Liver Cells in an Insulin-Dependent and Independent Manner

    Directory of Open Access Journals (Sweden)

    Chen Juan Gu

    2018-05-01

    Full Text Available Background/Aims: Obstructive sleep apnea is associated with diabetes and insulin resistance, but the underlying mechanisms remain unclear. The purpose of the current study was to determine the molecular effects of intermittent hypoxia (IH on hepatic insulin signaling and glucose homeostasis, and whether c-Jun NH2-terminal-kinase (JNK contributed to metabolic responses to IH in liver cells. Methods: The human HepG2 cells and rat FAO cells were exposed to 10, 30, 120, 240 or 360 cycles of IH (1% O2 for 60 s followed by 21% O2 for 60s, 7.5 cycles per hour or normoxia as a control. In a subgroup, we exposed cells to 360 cycles of IH with the JNK inhibitor SP600125. After IH exposure, cell glycogen content and glucose output were measured using colorimetric assay kits. Canonical insulin signaling and gluconeogenic genes were measured by western blot and quantitative polymerase chain reaction. Results: IH decreased insulin-stimulated protein kinase B (AKT/glycogen synthase kinase-3β (GSK-3β phosphorylation in a time-dependent manner, while inhibiting forkhead box protein O1 (FOXO1 expression and phosphoenolpyruvate carboxykinase (PEPCK transcription independent of insulin signaling. JNK inhibitor SP600125 partially restored AKT/ GSK-3β phosphorylation and glycogen synthesis, but did not affect other IH-induced glucose metabolic changes. Conclusion: IH in vitro impaired insulin signal transduction in liver cells as assessed by inhibited AKT/GSK-3β phosphorylation via JNK activation. IH inhibited FOXO1 and gluconeogenesis in an insulin-independent manner.

  14. L-carnosine affects the growth of Saccharomyces cerevisiae in a metabolism-dependent manner.

    Directory of Open Access Journals (Sweden)

    Stephanie P Cartwright

    Full Text Available The dipeptide L-carnosine (β-alanyl-L-histidine has been described as enigmatic: it inhibits growth of cancer cells but delays senescence in cultured human fibroblasts and extends the lifespan of male fruit flies. In an attempt to understand these observations, the effects of L-carnosine on the model eukaryote, Saccharomyces cerevisiae, were examined on account of its unique metabolic properties; S. cerevisiae can respire aerobically, but like some tumor cells, it can also exhibit a metabolism in which aerobic respiration is down regulated. L-Carnosine exhibited both inhibitory and stimulatory effects on yeast cells, dependent upon the carbon source in the growth medium. When yeast cells were not reliant on oxidative phosphorylation for energy generation (e.g. when grown on a fermentable carbon source such as 2% glucose, 10-30 mM L-carnosine slowed growth rates in a dose-dependent manner and increased cell death by up to 17%. In contrast, in media containing a non-fermentable carbon source in which yeast are dependent on aerobic respiration (e.g. 2% glycerol, L-carnosine did not provoke cell death. This latter observation was confirmed in the respiratory yeast, Pichia pastoris. Moreover, when deletion strains in the yeast nutrient-sensing pathway were treated with L-carnosine, the cells showed resistance to its inhibitory effects. These findings suggest that L-carnosine affects cells in a metabolism-dependent manner and provide a rationale for its effects on different cell types.

  15. Investigation of organic matter migrating from polymeric pipes into drinking water under different flow manners.

    Science.gov (United States)

    Zhang, Ling; Liu, Shuming; Liu, Wenjun

    2014-02-01

    Polymeric pipes, such as unplasticized polyvinyl chloride (uPVC) pipes, polypropylene random (PPR) pipes and polyethylene (PE) pipes are increasingly used for drinking water distribution lines. Plastic pipes may include some additives like metallic stabilizers and other antioxidants for the protection of the material during its production and use. Thus, some compounds can be released from those plastic pipes and cast a shadow on drinking water quality. This work develops a new procedure to investigate three types of polymer pipes (uPVC, PE and PPR) with respect to the migration of total organic carbon (TOC) into drinking water. The migration test was carried out in stagnant conditions with two types of migration processes, a continuous migration process and a successive migration process. These two types of migration processes are specially designed to mimic the conditions of different flow manners in drinking water pipelines, i.e., the situation of continuous stagnation with long hydraulic retention times and normal flow status with regular water renewing in drinking water networks. The experimental results showed that TOC release differed significantly with different plastic materials and under different flow manners. The order of materials with respect to the total amount of TOC migrating into drinking water was observed as PE > PPR > uPVC under both successive and continuous migration conditions. A higher amount of organic migration from PE and PPR pipes was likely to occur due to more organic antioxidants being used in pipe production. The results from the successive migration tests indicated the trend of the migration intensity of different pipe materials over time, while the results obtained from the continuous migration tests implied that under long stagnant conditions, the drinking water quality could deteriorate quickly with the consistent migration of organic compounds and the dramatic consumption of chlorine to a very low level. Higher amounts of TOC

  16. Public Schools

    Data.gov (United States)

    Department of Homeland Security — This Public Schools feature dataset is composed of all Public elementary and secondary education in the United States as defined by the Common Core of Data, National...

  17. Public acceptability

    International Nuclear Information System (INIS)

    Bolter, H.E.

    1989-01-01

    An urgent need to rebuild public confidence after an incident attracting widespread adverse publicity led to the development by British Nuclear Fuels plc of a completely new approach to public relations. The Company's experience suggests that impressions count more than sheer information, provided the impressions have a firm base in reality. (author)

  18. THE CHANGING DIMENSIONS OF PUBLIC RELATIONS: THE RELATIONSHIP BETWEEN BRAND MANAGEMENT AND PUBLIC RELATIONS

    OpenAIRE

    KÖKER, E. PELİN BAYTEKİN MİNE YENİÇERİ ALEMDAR

    2008-01-01

    The changes in the dimensions of public relations, due to the globalization effect on the business enterprises, are remarkable. In this manner, the relationship of public relations with re-engineering, total quality management, six sigma approach, event management, crisis management, reputation management, knowledge management and customer relationship management is evaluated in this study. Moreover, after establishing these interactions, the relationship between public relations and brand ma...

  19. Habitual Chocolate Consumption May Increase Body Weight in a Dose-Response Manner

    Science.gov (United States)

    Greenberg, James A.; Buijsse, Brian

    2013-01-01

    Objective Habitual chocolate intake was recently found to be associated with lower body weight in three cross-sectional epidemiological studies. Our objective was to assess whether these cross-sectional results hold up in a more rigorous prospective analysis. Methods We used data from the Atherosclerosis Risk in Communities cohort. Usual dietary intake was assessed by questionnaire at baseline (1987–98), and after six years. Participants reported usual chocolate intake as the frequency of eating a 1-oz (∼28 g) serving. Body weight and height were measured at the two visits. Missing data were replaced by multiple imputation. Linear mixed-effects models were used to evaluate cross-sectional and prospective associations between chocolate intake and adiposity. Results Data were from 15,732 and 12,830 participants at the first and second visit, respectively. More frequent chocolate consumption was associated with a significantly greater prospective weight gain over time, in a dose-response manner. For instance, compared to participants who ate a chocolate serving less often than monthly, those who ate it 1–4 times a month and at least weekly experienced an increase in Body Mass Index (kg/m2) of 0.26 (95% CI 0.08, 0.44) and 0.39 (0.23, 0.55), respectively, during the six-year study period. In cross-sectional analyses the frequency of chocolate consumption was inversely associated with body weight. This inverse association was attenuated after excluding participants with preexisting obesity-related illness. Compared to participants without such illness, those with it had higher BMI and reported less frequent chocolate intake, lower caloric intake, and diets richer in fruits and vegetables. They tended to make these dietary changes after becoming ill. Conclusions Our prospective analysis found that a chocolate habit was associated with long-term weight gain, in a dose-response manner. Our cross-sectional finding that chocolate intake was associated with lower body

  20. Habitual chocolate consumption may increase body weight in a dose-response manner.

    Science.gov (United States)

    Greenberg, James A; Buijsse, Brian

    2013-01-01

    Habitual chocolate intake was recently found to be associated with lower body weight in three cross-sectional epidemiological studies. Our objective was to assess whether these cross-sectional results hold up in a more rigorous prospective analysis. We used data from the Atherosclerosis Risk in Communities cohort. Usual dietary intake was assessed by questionnaire at baseline (1987-98), and after six years. Participants reported usual chocolate intake as the frequency of eating a 1-oz (~28 g) serving. Body weight and height were measured at the two visits. Missing data were replaced by multiple imputation. Linear mixed-effects models were used to evaluate cross-sectional and prospective associations between chocolate intake and adiposity. Data were from 15,732 and 12,830 participants at the first and second visit, respectively. More frequent chocolate consumption was associated with a significantly greater prospective weight gain over time, in a dose-response manner. For instance, compared to participants who ate a chocolate serving less often than monthly, those who ate it 1-4 times a month and at least weekly experienced an increase in Body Mass Index (kg/m2) of 0.26 (95% CI 0.08, 0.44) and 0.39 (0.23, 0.55), respectively, during the six-year study period. In cross-sectional analyses the frequency of chocolate consumption was inversely associated with body weight. This inverse association was attenuated after excluding participants with preexisting obesity-related illness. Compared to participants without such illness, those with it had higher BMI and reported less frequent chocolate intake, lower caloric intake, and diets richer in fruits and vegetables. They tended to make these dietary changes after becoming ill. Our prospective analysis found that a chocolate habit was associated with long-term weight gain, in a dose-response manner. Our cross-sectional finding that chocolate intake was associated with lower body weight did not apply to participants without

  1. Habitual chocolate consumption may increase body weight in a dose-response manner.

    Directory of Open Access Journals (Sweden)

    James A Greenberg

    Full Text Available OBJECTIVE: Habitual chocolate intake was recently found to be associated with lower body weight in three cross-sectional epidemiological studies. Our objective was to assess whether these cross-sectional results hold up in a more rigorous prospective analysis. METHODS: We used data from the Atherosclerosis Risk in Communities cohort. Usual dietary intake was assessed by questionnaire at baseline (1987-98, and after six years. Participants reported usual chocolate intake as the frequency of eating a 1-oz (~28 g serving. Body weight and height were measured at the two visits. Missing data were replaced by multiple imputation. Linear mixed-effects models were used to evaluate cross-sectional and prospective associations between chocolate intake and adiposity. RESULTS: Data were from 15,732 and 12,830 participants at the first and second visit, respectively. More frequent chocolate consumption was associated with a significantly greater prospective weight gain over time, in a dose-response manner. For instance, compared to participants who ate a chocolate serving less often than monthly, those who ate it 1-4 times a month and at least weekly experienced an increase in Body Mass Index (kg/m2 of 0.26 (95% CI 0.08, 0.44 and 0.39 (0.23, 0.55, respectively, during the six-year study period. In cross-sectional analyses the frequency of chocolate consumption was inversely associated with body weight. This inverse association was attenuated after excluding participants with preexisting obesity-related illness. Compared to participants without such illness, those with it had higher BMI and reported less frequent chocolate intake, lower caloric intake, and diets richer in fruits and vegetables. They tended to make these dietary changes after becoming ill. CONCLUSIONS: Our prospective analysis found that a chocolate habit was associated with long-term weight gain, in a dose-response manner. Our cross-sectional finding that chocolate intake was associated with

  2. Challenges of local public services management through public-private partnership

    Directory of Open Access Journals (Sweden)

    Violeta TINCU

    2016-12-01

    Full Text Available Under the legislation currently in force in the Republic of Moldova, public administration shall not bear the exclusive “burden” of organizing and managing public services on its own. Such a responsibility could be delegated to subjects outside the system of public administration. Scholars in the field call it an “indirect or delegated management” of public services. Public-private partnership is a new manner of delegating public services management. International experience has proven that such a partnership can be beneficial for public administration, contributing to improvements in public services quality. The reason is obvious: the private sector is competitive and client/user-oriented. However, the public-private partnership, which are not sufficiently operated in the legislation in force, also involves serious risks for the public partners.

  3. The canonical Wnt signaling pathway promotes chondrocyte differentiation in a Sox9-dependent manner

    International Nuclear Information System (INIS)

    Yano, Fumiko; Kugimiya, Fumitaka; Ohba, Shinsuke; Ikeda, Toshiyuki; Chikuda, Hirotaka; Ogasawara, Toru; Ogata, Naoshi; Takato, Tsuyoshi; Nakamura, Kozo; Kawaguchi, Hiroshi; Chung, Ung-il

    2005-01-01

    To better understand the role of the canonical Wnt signaling pathway in cartilage development, we adenovirally expressed a constitutively active (Canada) or a dominant negative (dn) form of lymphoid enhancer factor-1 (LEF-1), the main nuclear effector of the pathway, in undifferentiated mesenchymal cells, chondrogenic cells, and primary chondrocytes, and examined the expression of markers for chondrogenic differentiation and hypertrophy. caLEF-1 and LiCl, an activator of the canonical pathway, promoted both chondrogenic differentiation and hypertrophy, whereas dnLEF-1 and the gene silencing of β-catenin suppressed LiCl-promoted effects. To investigate whether these effects were dependent on Sox9, a master regulator of cartilage development, we stimulated Sox9-deficient ES cells with the pathway. caLEF-1 and LiCl promoted both chondrogenic differentiation and hypertrophy in wild-type, but not in Sox9-deficient, cells. The response of Sox9-deficient cells was restored by the adenoviral expression of Sox9. Thus, the canonical Wnt signaling pathway promotes chondrocyte differentiation in a Sox9-dependent manner

  4. Stat3 signaling regulates embryonic stem cell fate in a dose-dependent manner

    Directory of Open Access Journals (Sweden)

    Chih-I Tai

    2014-09-01

    Full Text Available Stat3 is essential for mouse embryonic stem cell (mESC self-renewal mediated by LIF/gp130 receptor signaling. Current understanding of Stat3-mediated ESC self-renewal mechanisms is very limited, and has heretofore been dominated by the view that Stat3 signaling functions in a binary “on/off” manner. Here, in contrast to this binary viewpoint, we demonstrate a contextual, rheostat-like mechanism for Stat3's function in mESCs. Activation and expression levels determine whether Stat3 functions in a self-renewal or a differentiation role in mESCs. We also show that Stat3 induces rapid differentiation of mESCs toward the trophectoderm (TE lineage when its activation level exceeds certain thresholds. Stat3 induces this differentiation phenotype via induction of Tfap2c and its downstream target Cdx2. Our findings provide a novel concept in the realm of Stat3, self-renewal signaling, and pluripotent stem cell biology. Ultimately, this finding may facilitate the development of conditions for the establishment of authentic non-rodent ESCs.

  5. The H3K27 demethylase, Utx, regulates adipogenesis in a differentiation stage-dependent manner.

    Directory of Open Access Journals (Sweden)

    Kazushige Ota

    Full Text Available Understanding the molecular mechanisms that drive adipogenesis is important in developing new treatments for obesity and diabetes. Epigenetic regulations determine the capacity of adipogenesis. In this study, we examined the role of a histone H3 lysine 27 demethylase, the ubiquitously transcribed tetratricopeptide repeat protein on the X chromosome (Utx, in the differentiation of mouse embryonic stem cells (mESCs to adipocytes. Using gene trapping, we examined Utx-deficient male mESCs to determine whether loss of Utx would enhance or inhibit the differentiation of mESCs to adipocytes. Utx-deficient mESCs showed diminished potential to differentiate to adipocytes compared to that of controls. In contrast, Utx-deficient preadipocytes showed enhanced differentiation to adipocytes. Microarray analyses indicated that the β-catenin/c-Myc signaling pathway was differentially regulated in Utx-deficient cells during adipocyte differentiation. Therefore, our data suggest that Utx governs adipogenesis by regulating c-Myc in a differentiation stage-specific manner and that targeting the Utx signaling pathway could be beneficial for the treatment of obesity, diabetes, and congenital utx-deficiency disorders.

  6. The Probiotic Lactobacillus Prevents Citrobacter rodentium-Induced Murine Colitis in a TLR2-Dependent Manner.

    Science.gov (United States)

    Ryu, Seung-Hyun; Park, Jong-Hyung; Choi, Soo-Young; Jeon, Hee-Yeon; Park, Jin-Il; Kim, Jun-Young; Ham, Seung-Hoon; Choi, Yang-Kyu

    2016-07-28

    The main objective of this study was to investigate whether Lactobacillus rhamnosus GG (LGG) ameliorated the effects of Citrobactor rodentium infection in Toll-like receptor 2 (TLR2) knockout (KO) and TLR4 KO mice, as well as in wild-type C57BL/6 (B6) mice. TLR2 KO, TLR4 KO, and B6 mice were divided into three groups per each strain. Each group had an uninfected control group (n = 5), C. rodentium-infected group (n = 8), and LGG-pretreated C. rodentium-infected group (n = 8). The survival rate of B6 mice infected with C. rodentium was higher when pretreated with LGG. Pretreatment with LGG ameliorated C. rodentium-induced mucosal hyperplasia in B6 and TLR4 KO mice. However, in C-rodentium-infected TLR2 KO mice, mucosal hyperplasia persisted, regardless of pretreatment with LGG. In addition, LGG-pretreated B6 and TLR4 KO mice showed a decrease in spleen weight and downregulation of tumor necrosis factor alpha, interferon gamma, and monocyte chemotactic protein 1 mRNA expression compared with the non-pretreated group. In contrast, such changes were not observed in TLR2 KO mice, regardless of pretreatment with LGG. From the above results, we conclude that pretreatment with LGG ameliorates C. rodentium-induced colitis in B6 and TLR4 KO mice, but not in TLR2 KO mice. Therefore, LGG protects mice from C. rodentium-induced colitis in a TLR2-dependent manner.

  7. The aPKC-CBP Pathway Regulates Adult Hippocampal Neurogenesis in an Age-Dependent Manner

    Directory of Open Access Journals (Sweden)

    Ayden Gouveia

    2016-10-01

    Full Text Available While epigenetic modifications have emerged as attractive substrates to integrate environmental changes into the determination of cell identity and function, specific signals that directly activate these epigenetic modifications remain unknown. Here, we examine the role of atypical protein kinase C (aPKC-mediated Ser436 phosphorylation of CBP, a histone acetyltransferase, in adult hippocampal neurogenesis and memory. Using a knockin mouse strain (CbpS436A in which the aPKC-CBP pathway is deficient, we observe impaired hippocampal neuronal differentiation, maturation, and memory and diminished binding of CBP to CREB in 6-month-old CbpS436A mice, but not at 3 months of age. Importantly, elevation of CREB activity rescues these deficits, and CREB activity is reduced whereas aPKC activity is increased in the murine hippocampus as they age from 3 to 6 months regardless of genotype. Thus, the aPKC-CBP pathway is a homeostatic compensatory mechanism that modulates hippocampal neurogenesis and memory in an age-dependent manner in response to reduced CREB activity.

  8. Fesselin is a target protein for calmodulin in a calcium-dependent manner

    International Nuclear Information System (INIS)

    KoIakowski, Janusz; Wrzosek, Antoni; Dabrowska, Renata

    2004-01-01

    Fesselin is a basic protein isolated from smooth muscle which binds G-actin and accelerates its polymerization as well as cross-links assembled filaments [J. Muscle Res. Cell Motil. 20 (1999) 539; Biochemistry 40 (2001) 14252]. In this report experimental evidence is provided for the first time proving that fesselin can interact with calmodulin in a Ca 2+ -dependent manner in vitro. Using ion exchange, followed by calmodulin-affinity chromatography, enabled us to simplify and shorten the fesselin preparation procedure and increase its yield by about three times in comparison to the procedure described by Leinweber et al. [J. Muscle Res. Cell Motil. 20 (1999) 539]. Fesselin interaction with dansyl-labelled calmodulin causes a 2-fold increase in maximum fluorescence intensity of the fluorophore and a 21 nm blue shift of the spectrum. The transition of complex formation between fesselin and calmodulin occurs at submicromolar concentration of calcium ions. The dissociation constant of fesselin Ca 2+ /calmodulin complexes amounted to 10 -8 M. The results suggest the existence of a direct link between Ca 2+ /calmodulin and fesselin at the level of actin cytoskeleton dynamics in smooth muscle

  9. TGF-β's delay skeletal muscle progenitor cell differentiation in an isoform-independent manner

    International Nuclear Information System (INIS)

    Schabort, Elske J.; Merwe, Mathilde van der; Loos, Benjamin; Moore, Frances P.; Niesler, Carola U.

    2009-01-01

    Satellite cells are a quiescent heterogenous population of mononuclear stem and progenitor cells which, once activated, differentiate into myotubes and facilitate skeletal muscle repair or growth. The Transforming Growth Factor-β (TGF-β) superfamily members are elevated post-injury and their importance in the regulation of myogenesis and wound healing has been demonstrated both in vitro and in vivo. Most studies suggest a negative role for TGF-β on satellite cell differentiation. However, none have compared the effect of these three isoforms on myogenesis in vitro. This is despite known isoform-specific effects of TGF-β1, -β2 and -β3 on wound repair in other tissues. In the current study we compared the effect of TGF-β1, -β2 and -β3 on proliferation and differentiation of the C2C12 myoblast cell-line. We found that, irrespective of the isoform, TGF-β increased proliferation of C2C12 cells by changing the cellular localisation of PCNA to promote cell division and prevent cell cycle exit. Concomitantly, TGF-β1, -β2 and -β3 delayed myogenic commitment by increasing MyoD degradation and decreasing myogenin expression. Terminal differentiation, as measured by a decrease in myosin heavy chain (MHC) expression, was also delayed. These results demonstrate that TGF-β promotes proliferation and delays differentiation of C2C12 myoblasts in an isoform-independent manner

  10. Comprehending non-native speakers: theory and evidence for adjustment in manner of processing.

    Science.gov (United States)

    Lev-Ari, Shiri

    2014-01-01

    Non-native speakers have lower linguistic competence than native speakers, which renders their language less reliable in conveying their intentions. We suggest that expectations of lower competence lead listeners to adapt their manner of processing when they listen to non-native speakers. We propose that listeners use cognitive resources to adjust by increasing their reliance on top-down processes and extracting less information from the language of the non-native speaker. An eye-tracking study supports our proposal by showing that when following instructions by a non-native speaker, listeners make more contextually-induced interpretations. Those with relatively high working memory also increase their reliance on context to anticipate the speaker's upcoming reference, and are less likely to notice lexical errors in the non-native speech, indicating that they take less information from the speaker's language. These results contribute to our understanding of the flexibility in language processing and have implications for interactions between native and non-native speakers.

  11. Bilirubin Modulates Acetylcholine Receptors In Rat Superior Cervical Ganglionic Neurons In a Bidirectional Manner

    Science.gov (United States)

    Zhang, Chengmi; Wang, Zhenmeng; Dong, Jing; Pan, Ruirui; Qiu, Haibo; Zhang, Jinmin; Zhang, Peng; Zheng, Jijian; Yu, Weifeng

    2014-01-01

    Autonomic dysfunction as a partial contributing factor to cardiovascular instability in jaundiced patients is often associated with increased serum bilirubin levels. Whether increased serum bilirubin levels could directly inhibit sympathetic ganglion transmission by blocking neuronal nicotinic acetylcholine receptors (nAChRs) remains to be elucidated. Conventional patch-clamp recordings were used to study the effect of bilirubin on nAChRs currents from enzymatically dissociated rat superior cervical ganglia (SCG) neurons. The results showed that low concnetrations (0.5 and 2 μM) of bilirubin enhanced the peak ACh-evoked currents, while high concentrations (3 to 5.5 µM) of bilirubin suppressed the currents with an IC50 of 4 ± 0.5 μM. In addition, bilirubin decreased the extent of desensitization of nAChRs in a concentration-dependent manner. This inhibitory effect of bilirubin on nAChRs channel currents was non-competitive and voltage independent. Bilirubin partly improved the inhibitory effect of forskolin on ACh-induced currents without affecting the action of H-89. These data suggest that the dual effects of enhancement and suppression of bilirubin on nAChR function may be ascribed to the action mechanism of positive allosteric modulation and direct blockade. Thus, suppression of sympathetic ganglionic transmission through postganglionic nAChRs inhibition may partially contribute to the adverse cardiovascular effects in jaundiced patients. PMID:25503810

  12. MCP-1 expressed by osteoclasts stimulates osteoclastogenesis in an autocrine/paracrine manner

    International Nuclear Information System (INIS)

    Miyamoto, Kana; Ninomiya, Ken; Sonoda, Koh-Hei; Miyauchi, Yoshiteru; Hoshi, Hiroko; Iwasaki, Ryotaro; Miyamoto, Hiroya

    2009-01-01

    Monocyte chemoattractant protein-1 (MCP-1) is a chemokine that plays a critical role in the recruitment and activation of leukocytes. Here, we describe that multinuclear osteoclast formation was significantly inhibited in cells derived from MCP-1-deficient mice. MCP-1 has been implicated in the regulation of osteoclast cell-cell fusion; however defects of multinuclear osteoclast formation in the cells from mice deficient in DC-STAMP, a seven transmembrane receptor essential for osteoclast cell-cell fusion, was not rescued by recombinant MCP-1. The lack of MCP-1 in osteoclasts resulted in a down-regulation of DC-STAMP, NFATc1, and cathepsin K, all of which were highly expressed in normal osteoclasts, suggesting that osteoclast differentiation was inhibited in MCP-1-deficient cells. MCP-1 alone did not induce osteoclastogenesis, however, the inhibition of osteoclastogenesis in MCP-1-deficient cells was restored by addition of recombinant MCP-1, indicating that osteoclastogenesis was regulated in an autocrine/paracrine manner by MCP-1 under the stimulation of RANKL in osteoclasts.

  13. Paternal engagement during childbirth depending on the manner of their preparation.

    Science.gov (United States)

    Sioma-Markowska, Urszula; Poręba, Ryszard; Machura, Mariola; Skrzypulec-Plinta, Violetta

    2016-01-01

    The analysis of the forms of paternal activity depending on the manner of their preparation, including stages of labor. A prospective survey-based study involved 250 fathers who participated in their child's birth. The fathers included in the study were present during all stages of family-assisted natural labor. The study was conducted one day after childbirth with the use of a survey prepared by the authors. Statistical calculations were conducted using the Statistica PL software. The frequency of individual qualitative features (non-measurable) was assessed by means of a non-parametric χ² (chi-squared) test. The statistical significance level was p fathers included in the study (52.4%) participated in childbirth with no prior preparation. The dominant form of preparation involved self-education from books, magazines and the Internet (24%). 23.6% of fathers participated in ante-natal classes. The study demonstrated that fathers prepared for childbirth in ante-natal classes more often engaged in the supportive role, provided nursing care and carried out instrumental monitoring during each stage of childbirth. The fathers prepared for childbirth in ante-natal classes more often engage in the supportive role, provide nursing care and carry out instrumental control during each stage of childbirth. Ante-natal classes should be promoted as an optimal form of preparation for active participation in childbirth. Moreover, other forms of paternal ante-natal education as well as continued education in a delivery room should be developed.

  14. Nuclear size is sensitive to NTF2 protein levels in a manner dependent on Ran binding

    Science.gov (United States)

    Vuković, Lidija D.; Jevtić, Predrag; Zhang, Zhaojie; Stohr, Bradley A.; Levy, Daniel L.

    2016-01-01

    ABSTRACT Altered nuclear size is associated with many cancers, and determining whether cancer-associated changes in nuclear size contribute to carcinogenesis necessitates an understanding of mechanisms of nuclear size regulation. Although nuclear import rates generally positively correlate with nuclear size, NTF2 levels negatively affect nuclear size, despite the role of NTF2 (also known as NUTF2) in nuclear recycling of the import factor Ran. We show that binding of Ran to NTF2 is required for NTF2 to inhibit nuclear expansion and import of large cargo molecules in Xenopus laevis egg and embryo extracts, consistent with our observation that NTF2 reduces the diameter of the nuclear pore complex (NPC) in a Ran-binding-dependent manner. Furthermore, we demonstrate that ectopic NTF2 expression in Xenopus embryos and mammalian tissue culture cells alters nuclear size. Finally, we show that increases in nuclear size during melanoma progression correlate with reduced NTF2 expression, and increasing NTF2 levels in melanoma cells is sufficient to reduce nuclear size. These results show a conserved capacity for NTF2 to impact on nuclear size, and we propose that NTF2 might be a new cancer biomarker. PMID:26823604

  15. Maternal obesity programs increased leptin gene expression in rat male offspring via epigenetic modifications in a depot-specific manner

    Directory of Open Access Journals (Sweden)

    Simon Lecoutre

    2017-08-01

    Conclusions: Consistent with the DOHaD hypothesis, persistent epigenetic remodeling occurs at regulatory regions especially within intergenic sequences, linked to higher leptin gene expression in adult HF offspring in a depot-specific manner.

  16. Academic Publications

    OpenAIRE

    Francisco H C Felix

    2017-01-01

    Alternative modes of academic publication. What it is: Page for the dissemination of academic papers in alternative formats. Aimed at the diffusion of the idea of open publication, or open access publication, a branch of open science, a multidisciplinary movement that seeks to modify the paradigm of knowledge production that centralizes it and prevents its spreading. Historically, Western tradition has become firmly rooted in the free dissemination of knowledge among peers. However, the c...

  17. Public transport

    OpenAIRE

    Lethbridge, Jane

    2008-01-01

    Public transport plays an essential role in enabling people from low income and other disadvantaged groups to access employment and services. It also contributes to the development of social networks and social capital, by helping people to visit friends and relatives and take part in community and other social activities. Public policy makers have begun to recognise that adequate public transport provision can play an important role in reducing social exclusion. [Taken from introductory para...

  18. Public acceptance: A Japanese view

    International Nuclear Information System (INIS)

    1972-01-01

    A number of factors enter into a consideration of the public acceptance of nuclear power ? the public, nuclear power as an entity, and the interaction between the two. Interaction here implies the manner in which nuclear power is presented to the public ? what is the public need for nuclear power, and what public risk is entailed in having it? The problem of public acceptance, in this sense, is time-dependent. For the public is changeable, just as nuclear power is subject to technical progress and ' social' improvement. Japan is geographically a very small country with a very high density of population. Any industrial activity and any large-scale employment of modern technology is apt to have a much greater impact on the physical, social and biological environment of individual Japanese people than similar activities would have on those of other countries. Industrial pollutants such as sulphur dioxide from power plants, oxides of nitrogen from automobile engine exhausts, organic mercury from chemical industries and so on affect society to a high degree, considered in terms of their concentration either per capita or per square kilometre. In the case of nuclear power, therefore, people are more concerned with radiological effects than with thermal pollution.no matter how one looks at it, the experience of Hiroshima and Nagasaki has made the average member of the Japanese public, very sensitive to the problem of radiation safety. This is no longer a subject in which science or logic can persuade

  19. Public acceptance: A Japanese view

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1972-07-01

    A number of factors enter into a consideration of the public acceptance of nuclear power ? the public, nuclear power as an entity, and the interaction between the two. Interaction here implies the manner in which nuclear power is presented to the public ? what is the public need for nuclear power, and what public risk is entailed in having it? The problem of public acceptance, in this sense, is time-dependent. For the public is changeable, just as nuclear power is subject to technical progress and ' social' improvement. Japan is geographically a very small country with a very high density of population. Any industrial activity and any large-scale employment of modern technology is apt to have a much greater impact on the physical, social and biological environment of individual Japanese people than similar activities would have on those of other countries. Industrial pollutants such as sulphur dioxide from power plants, oxides of nitrogen from automobile engine exhausts, organic mercury from chemical industries and so on affect society to a high degree, considered in terms of their concentration either per capita or per square kilometre. In the case of nuclear power, therefore, people are more concerned with radiological effects than with thermal pollution.no matter how one looks at it, the experience of Hiroshima and Nagasaki has made the average member of the Japanese public, very sensitive to the problem of radiation safety. This is no longer a subject in which science or logic can persuade.

  20. Proliferation marker pKi-67 affects the cell cycle in a self-regulated manner.

    Science.gov (United States)

    Schmidt, Mirko H H; Broll, Rainer; Bruch, Hans-Peter; Duchrow, Michael

    2002-01-01

    The proliferation marker pKi-67 is commonly used in research and pathology to detect proliferating cells. In a previous work, we found the protein to be associated with regulators of the cell cycle, controlling S-phase progression, as well as entry into and exit from mitosis. Here we investigate whether pKi-67 has a regulative effect on the cell cycle itself. For that purpose we cloned four fragments of pKi-67, together representing nearly the whole protein, and an N-terminal pKi-67 antisense oligonucleotide into a tetracycline inducible gene expression system. The sense fragments were C-terminally modified by addition of either a nuclear localization sequence (NLS) or a STOP codon to address the impact of their intracellular distribution. FACS based cell cycle analysis revealed that expression of nearly all pKi-67 domains and the antisense oligonucleotide led to a decreased amount of cells in S-phase and an increased number of cells in G(2)/M- and G(1)-phase. Subsequent analysis of the endogenous pKi-67 mRNA and protein levels revealed that the constructs with the most significant impact on the cell cycle were able to silence pKi-67 transcription as well. We conclude from the data that pKi-67 influences progression of S-phase and mitosis in a self-regulated manner and, therefore, effects the cell cycle checkpoints within both phases. Furthermore, we found pKi-67 mediates an anti-apoptotic effect on the cell and we verified that this marker, although it is a potential ribosomal catalyst, is not expressed in differentiated tissues with a high transcriptional activity. Copyright 2002 Wiley-Liss, Inc.

  1. Isoproterenol Increases RANKL Expression in a ATF4/NFATc1-Dependent Manner in Mouse Osteoblastic Cells

    Directory of Open Access Journals (Sweden)

    Kyunghwa Baek

    2017-10-01

    Full Text Available Sympathetic nervous system stimulation-induced β-adrenergic signal transduction is known to induce bone loss and increase of osteoclast activity. Although isoproterenol, a nonspecific β-adrenergic receptor agonist, has been shown to increase receptor activator of NF-κB ligand (RANKL, the details of the regulatory mechanisms remain unclear. In the present study, we investigated the role of the nuclear factor of activated T-cells (NFAT in isoproterenol-induced RANKL expression in C2C12 and in primary cultured mouse calvarial cells. Isoproterenol increased nuclear factor of activated T-cells cytoplasmic 1 (NFATc1 and RANKL expressions at both mRNA and protein levels and increased NFAT reporter activity. NFATc1 knockdown blocked isoproterenol-mediated RANKL expression. Isoproterenol also promoted cAMP response element-binding protein 1 (CREB1 and activating transcription factor 4 (ATF4 phosphorylation. Isoproterenol-mediated transcriptional activation of NFAT was blocked by protein kinase A (PKA inhibitor H89. Isoproterenol-induced CREB1, ATF4, NFATc1, and RANKL expressions were suppressed by H89. Mutations in cAMP response element-like or NFAT-binding element suppressed isoproterenol-induced RANKL promoter activity. Chromatin immunoprecipitation analysis demonstrated that isoproterenol increased NFAT-binding and ATF4-binding activities on the mouse RANKL promoter, but did not increase CREB1-binding activity. Association of NFATc1 and ATF4 was not observed in a co-immunoprecipitation study. ATF4 knockdown suppressed isoproterenol-induced NFAT binding to the RANKL promoter, whereas NFATc1 knockdown did not suppress isoproterenol-induced ATF4 binding to the RANKL promoter. ATF4 knockdown suppressed isoproterenol-induced expressions of NFATc1 and RANKL. These results suggest that isoproterenol increases RANKL expression in an ATF4/NFATc1-dependent manner.

  2. Relationship between the Manner of Mobile Phone Use and Depression, Anxiety, and Stress in University Students.

    Science.gov (United States)

    Višnjić, Aleksandar; Veličković, Vladica; Sokolović, Dušan; Stanković, Miodrag; Mijatović, Kristijan; Stojanović, Miodrag; Milošević, Zoran; Radulović, Olivera

    2018-04-08

    Objectives : There is insufficient evidence regarding the potential risk of mobile phone use on mental health. Therefore, the aim of this research was to examine the relationship between mobile phone use and mental health by measuring the levels of depression, anxiety, and stress among university students in Serbia and Italy. Methods : This cross-sectional study was carried out at two distinguished universities in Serbia and Italy from March to May of the 2015/2016 academic year and included 785 students of both genders. The questionnaire was compiled and developed from different published sources regarding the manner and intensity of mobile phone use, along with the Depression Anxiety Stress Scale (DASS 42) for measuring psychological health. The statistical analysis of the data included the application of binary logistic regression and correlation tests. Results: Statistical analysis indicates that anxiety symptoms are somewhat more present in younger students (odds ratio (OR) = 0.86, 95% confidence interval (CI): 0.76-0.96), in those who send more text messages SMSs (OR = 1.15, 95% CI: 1.11-1.31), and in those who browse the internet less frequently (OR = 0.84, 95% CI: 0.73-0.95). Stress is more common in students who make fewer calls a day (OR = 0.79, 95% CI: 0.64-0.97), as well in those who spend more time talking on the mobile phone per day (OR = 1.28, 95% CI: 1.12-1.56). The strongest predictor of high stress levels was keeping the mobile phone less than 1 m away during sleeping (OR = 1.48, 95% CI: 1.12-2.08). Conclusions: The results indicated that the intensity and modality of mobile phone use could be a factor that can influence causal pathways leading to mental health problems in the university student population.

  3. Adiponectin influences progesterone production from MA-10 Leydig cells in a dose-dependent manner.

    Science.gov (United States)

    Landry, David; Paré, Aurélie; Jean, Stéphanie; Martin, Luc J

    2015-04-01

    Obesity in men is associated with lower testosterone levels, related to reduced sperm concentration and the development of various diseases with aging. Hormones produced by the adipose tissue may have influences on both metabolism and reproductive function. Among them, the production and secretion of adiponectin is inversely correlated to total body fat. Adiponectin receptors (AdipoR1 and AdipoR2) have been found to be expressed in testicular Leydig cells (producing testosterone). Since StAR and Cyp11a1 are essential for testosterone synthesis and adiponectin has been shown to regulate StAR mRNA in swine granulosa cells, we hypothesized that adiponectin might also regulate these genes in Leydig cells. Our objective was to determine whether adiponectin regulates StAR and Cyp11a1 genes in Leydig cells and to better define its mechanisms of action. Methods used in the current study are qPCR for the mRNA levels, transfections for promoter activities, and enzyme-linked immunosorbent assay for the progesterone concentration. We have found that adiponectin cooperates with cAMP-dependent stimulation to activate StAR and Cyp11a1 mRNA expressions in a dose-dependent manner in MA-10 Leydig cells as demonstrated by transfection of a luciferase reporter plasmid. These results led to a significant increase in progesterone production from MA-10 cells. Thus, our data suggest that high doses of adiponectin typical of normal body weight may promote testosterone production from Leydig cells.

  4. IGFBP-4 regulates adult skeletal growth in a sex-specific manner.

    Science.gov (United States)

    Maridas, David E; DeMambro, Victoria E; Le, Phuong T; Nagano, Kenichi; Baron, Roland; Mohan, Subburaman; Rosen, Clifford J

    2017-04-01

    Insulin-like growth factor-1 (IGF-1) and its binding proteins are critical mediators of skeletal growth. Insulin-like growth factor-binding protein 4 (IGFBP-4) is highly expressed in osteoblasts and inhibits IGF-1 actions in vitro Yet, in vivo studies suggest that it could potentiate IGF-1 and IGF-2 actions. In this study, we hypothesized that IGFBP-4 might potentiate the actions of IGF-1 on the skeleton. To test this, we comprehensively studied 8- and 16-week-old Igfbp4 -/- mice. Both male and female adult Igfbp4 -/- mice had marked growth retardation with reductions in body weight, body and femur lengths, fat proportion and lean mass at 8 and 16 weeks. Marked reductions in aBMD and aBMC were observed in 16-week-old Igfbp4 -/- females, but not in males. Femoral trabecular BV/TV and thickness, cortical fraction and thickness in 16-week-old Igfbp4 -/- females were significantly reduced. However, surprisingly, males had significantly more trabeculae with higher connectivity density than controls. Concordantly, histomorphometry revealed higher bone resorption and lower bone formation in Igfbp4 -/- females. In contrast, Igfbp4 -/- males had lower mineralized surface/bone surface. Femoral expression of Sost and circulating levels of sclerostin were reduced but only in Igfbp4 -/- males. Bone marrow stromal cultures from mutants showed increased osteogenesis, whereas osteoclastogenesis was markedly increased in cells from Igfbp4 -/- females but decreased in males. In sum, our results indicate that loss of Igfbp4 affects mesenchymal stromal cell differentiation, regulates osteoclastogenesis and influences both skeletal development and adult bone maintenance. Thus, IGFBP-4 modulates the skeleton in a gender-specific manner, acting as both a cell autonomous and cell non-autonomous factor. © 2017 The authors.

  5. A time-dependent degeneration manner of condyle in rat CFA-induced inflamed TMJ.

    Science.gov (United States)

    Xu, Liqin; Guo, Huilin; Li, Cheng; Xu, Jie; Fang, Wei; Long, Xing

    2016-01-01

    Temporomandibular joint (TMJ) inflammation is a potential risk factor of osteoarthritis (OA) but the detailed degenerative changes in the inflamed TMJ remain unclear. In this study, we evaluated the changes of condylar cartilage and subchondral bone in rat inflamed TMJ induced by Freund's complete adjuvant (CFA). Articular cavity was injected with CFA and the TMJ samples were collected 1, 2, 3, and 4-week post-injection. Hematoxylin & Eosin (H&E) staining, toluidine blue (TB) staining, Safranin O (S.O) staining, Masson trichrome staining and micro-CT were used to assess TMJ degeneration during inflammation. Osteoclast and osteoblast activities were analyzed by tartrate-resistant acid phosphatase (TRAP) staining and osteocalcin (OCN) immunohistochemistry staining respectively. The expression of receptor activator of NF-kB ligand (RANKL) and osteoprotegerin (OPG) in condylar cartilage and subchondral bone was also evaluated through immunohistochemistry and RANKL/OPG ratio was evaluated. Reduced cartilage thickness, decreased number of chondrocytes, and down-regulated proteoglycan expression were observed in the condylar cartilage in the inflamed TMJ. Enhanced osteoclast activity, and expanded bone marrow cavity were reached the peak in the 2-week after CFA-injection. Meanwhile the RANKL/OPG ratio in the cartilage and subchondral bone also increased in the 2-week CFA-injection. Immature, unmineralized new bones with irregular trabecular bone structure, atypical condylar shape, up-regulated OCN expression, and decreased bone mineral density (BMD) were found in the inflamed TMJ. The time-dependent degeneration manner of TMJ cartilage and subchondral bone was found in CFA-induced arthritis rat model. The degeneration in the TMJ with inflammation might be a risk factor and should be concerned.

  6. Apolipoprotein A-IV constrains HPA and behavioral stress responsivity in a strain-dependent manner.

    Science.gov (United States)

    Packard, Amy E B; Zhang, Jintao; Myers, Brent; Ko, Chih-Wei; Wang, Fei; Tso, Patrick; Ulrich-Lai, Yvonne M

    2017-12-01

    There is a critical gap in our knowledge of the mechanisms that govern interactions between daily life experiences (e.g., stress) and metabolic diseases, despite evidence that stress can have profound effects on cardiometabolic health. Apolipoprotein A-IV (apoA-IV) is a protein found in chylomicrons (lipoprotein particles that transport lipids throughout the body) where it participates in lipid handling and the regulation of peripheral metabolism. Moreover, apoA-IV is expressed in brain regions that regulate energy balance including the arcuate nucleus. Given that both peripheral and central metabolic processes are important modulators of hypothalamic-pituitary-adrenocortical (HPA) axis activity, the present work tests the hypothesis that apoA-IV activity affects stress responses. As emerging data suggests that apoA-IV actions can vary with background strain, we also explore the strain-dependence of apoA-IV stress regulation. These studies assess HPA axis, metabolic (hyperglycemia), and anxiety-related behavioral responses to psychogenic stress in control (wildtype) and apoA-IV-deficient (KO) mice on either the C57Bl/6J (C57) or 129×1/SvJ (129) background strain. The results indicate that apoA-IV KO increases post-stress corticosterone and anxiety-related behavior specifically in the 129 strain, and increases stress-induced hyperglycemia exclusively in the C57 strain. These data support the hypothesis that apoA-IV is a novel factor that limits stress reactivity in a manner that depends on genetic background. An improved understanding of the complex relationship among lipid homeostasis, stress sensitivity, and genetics is needed to optimize the development of personalized treatments for stress- and metabolism-related diseases. Copyright © 2017 Elsevier Ltd. All rights reserved.

  7. Relationship between the Manner of Mobile Phone Use and Depression, Anxiety, and Stress in University Students

    Science.gov (United States)

    Višnjić, Aleksandar; Veličković, Vladica; Sokolović, Dušan; Stanković, Miodrag; Stojanović, Miodrag; Milošević, Zoran; Radulović, Olivera

    2018-01-01

    Objectives: There is insufficient evidence regarding the potential risk of mobile phone use on mental health. Therefore, the aim of this research was to examine the relationship between mobile phone use and mental health by measuring the levels of depression, anxiety, and stress among university students in Serbia and Italy. Methods: This cross-sectional study was carried out at two distinguished universities in Serbia and Italy from March to May of the 2015/2016 academic year and included 785 students of both genders. The questionnaire was compiled and developed from different published sources regarding the manner and intensity of mobile phone use, along with the Depression Anxiety Stress Scale (DASS 42) for measuring psychological health. The statistical analysis of the data included the application of binary logistic regression and correlation tests. Results: Statistical analysis indicates that anxiety symptoms are somewhat more present in younger students (odds ratio (OR) = 0.86, 95% confidence interval (CI): 0.76–0.96), in those who send more text messages (SMSs) (OR = 1.15, 95% CI: 1.11–1.31), and in those who browse the internet less frequently (OR = 0.84, 95% CI: 0.73–0.95). Stress is more common in students who make fewer calls a day (OR = 0.79, 95% CI: 0.64–0.97), as well in those who spend more time talking on the mobile phone per day (OR = 1.28, 95% CI: 1.12–1.56). The strongest predictor of high stress levels was keeping the mobile phone less than 1 m away during sleeping (OR = 1.48, 95% CI: 1.12–2.08). Conclusions: The results indicated that the intensity and modality of mobile phone use could be a factor that can influence causal pathways leading to mental health problems in the university student population. PMID:29642471

  8. Metformin decreases lung cancer risk in diabetic patients in a dose-dependent manner.

    Science.gov (United States)

    Tsai, Ming-Ju; Yang, Chih-Jen; Kung, Ya-Ting; Sheu, Chau-Chyun; Shen, Yu-Ting; Chang, Pi-Yu; Huang, Ming-Shyan; Chiu, Herng-Chia

    2014-11-01

    Higher risk of lung cancer has been noted in patients with type 2 diabetes mellitus (DM). Some observational studies have shown a reduced risk of lung cancer in DM patients taking metformin, but a dose-response relationship has never been reported. The aim of this study is to exam the association between the dose of metformin and the incidence of lung cancer in a Chinese population. The dataset used for this nationwide population-based study is a cohort of 1 million subjects randomly sampled from individuals enrolled in the Taiwan National Health Insurance system. We enrolled all subjects with newly diagnosed type 2 DM between 1997 and 2007. Subjects with a diagnosis of neoplasm before DM diagnosis, those using metformin before DM diagnosis, those with polycystic ovary syndrome, and those with a DM diagnosis before their 15 years of age were excluded. The demographic data and duration, cumulative dose and intensity of metformin use were compared between patients developing lung cancer and those without lung cancer. Totally, 47,356 subjects were identified. After adjusting for age, gender, and modified Charlson Comorbidity Index score, the utilization of metformin was an independent protecting factor, and the risk of developing lung cancer decreased progressively with either the higher cumulative dose or the higher intensity of metformin use. This study revealed that the use of metformin decreased the risk of lung cancer in a dose-dependent manner in patients with type 2 DM. The chemo-preventive effect of metformin deserves further study. Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

  9. Dopamine D3 receptor knockout mice exhibit abnormal nociception in a sex-different manner.

    Science.gov (United States)

    Liu, Peng; Xing, Bo; Chu, Zheng; Liu, Fei; Lei, Gang; Zhu, Li; Gao, Ya; Chen, Teng; Dang, Yong-Hui

    2017-07-01

    Pain is a complex and subjective experience. Previous studies have shown that mice lacking the dopamine D3 receptor (D3RKO) exhibit hypoalgesia, indicating a role of the D3 receptor in modulation of nociception. Given that there are sex differences in pain perception, there may be differences in responses to nociceptive stimuli between male and female D3RKO mice. In the current study, we examined the role of the D3 receptor in modulating nociception in male and female D3RKO mice. Acute thermal pain was modeled by hot-plate test. This test was performed at different temperatures including 52°C, 55°C, and 58°C. The von Frey hair test was applied to evaluate mechanical pain. And persistent pain produced by peripheral tissue injury and inflammation was modeled by formalin test. In the hot-plate test, compared with wild-type (WT) mice, D3RKO mice generally exhibited longer latencies at each of the three temperatures. Specially, male D3RKO mice showed hypoalgesia compared with male WT mice when the temperature was 55°C, while for the female mice, there was a statistical difference between genotypes when the test condition was 52°C. In the von Frey hair test, both male and female D3RKO mice exhibited hypoalgesia. In the formalin test, the male D3RKO mice displayed a similar nociceptive behavior as their sex-matched WT littermates, whereas significantly depressed late-phase formalin-induced nociceptive behaviors were observed in the female mutants. These findings indicated that the D3 receptor affects nociceptive behaviors in a sex-specific manner and that its absence induces more analgesic behavior in the female knockout mice. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

  10. Maternal prenatal cortisol predicts infant negative emotionality in a sex-dependent manner.

    Science.gov (United States)

    Braithwaite, Elizabeth C; Pickles, Andrew; Sharp, Helen; Glover, Vivette; O'Donnell, Kieran J; Tibu, Florin; Hill, Jonathan

    2017-06-01

    Prenatal stress influences fetal developmental trajectories, which may implicate glucocorticoid mechanisms. There is also emerging evidence that effects of prenatal stress on offspring development are sex-dependent. However, little is known about the prospective relationship between maternal prenatal cortisol levels and infant behaviour, and whether it may be different in male and female infants. We sought to address this question using data from a prospective longitudinal cohort, stratified by risk. The Wirral Child Health and Development Study (WCHADS) cohort (n=1233) included a stratified random sub-sample (n=216) who provided maternal saliva samples, assayed for cortisol, at home over two days at 32weeks of pregnancy (on waking, 30-min post-waking and during the evening) and a measure of infant negative emotionality from the Neonatal Behavioural Assessment Scale (NBAS) at five weeks-of-age. General population estimates of associations among measures were obtained using inverse probability weights. Maternal prenatal cortisol sampled on waking predicted infant negative emotionality in a sex-dependent manner (interaction term, p=0.005); female infants exposed to high levels of prenatal cortisol were more negative (Beta=0.440, p=0.042), whereas male infants were less negative (Beta=-0.407, p=0.045). There was no effect of the 30-min post-waking measure or evening cortisol. Our findings add to an emerging body of work that has highlighted sex differences in fetal programming, whereby females become more reactive following prenatal stress, and males less reactive. A more complete understanding of sex-specific developmental trajectories in the context of prenatal stress is essential for the development of targeted prevention strategies. Copyright © 2017. Published by Elsevier Inc.

  11. Listeria monocytogenes alters mast cell phenotype, mediator and osteopontin secretion in a listeriolysin-dependent manner.

    Directory of Open Access Journals (Sweden)

    Catherine E Jobbings

    Full Text Available Whilst mast cells participate in the immune defence against the intracellular bacterium Listeria monocytogenes, there is conflicting evidence regarding the ability of L. monocytogenes to infect mast cells. It is known that the pore-forming toxin listeriolysin (LLO is important for mast cell activation, degranulation and the release of pro-inflammatory cytokines. Mast cells, however, are a potential source of a wide range of cytokines, chemokines and other mediators including osteopontin, which contributes to the clearing of L. monocytogenes infections in vivo, although its source is unknown. We therefore aimed to resolve the controversy of mast cell infection by L. monocytogenes and investigated the extent of mediator release in response to the bacterium. In this paper we show that the infection of bone marrow-derived mast cells by L. monocytogenes is inefficient and LLO-independent. LLO, however, is required for calcium-independent mast cell degranulation as well as for the transient and selective downregulation of cell surface CD117 (c-kit on mast cells. We demonstrate that in addition to the key pro-inflammatory cytokines TNF-α and IL-6, mast cells release a wide range of other mediators in response to L. monocytogenes. Osteopontin, IL-2, IL-4, IL-13 and granulocyte macrophage colony-stimulating factor (GM-CSF, and chemokines including CCL2, CCL3, CCL4 and CCL5 are released in a MyD88-dependent manner. The wide range of mediators released by mast cells in response to L. monocytogenes may play an important role in the recruitment and activation of a variety of immune cells in vivo. The cocktail of mediators, however, is unlikely to skew the immune response to a particular effector response. We propose that mast cells provide a hitherto unreported source of osteopontin, and may provide an important role in co-ordinating the immune response during Listeria infection.

  12. Neuron-astrocyte interaction enhance GABAergic synaptic transmission in a manner dependent on key metabolic enzymes.

    Directory of Open Access Journals (Sweden)

    Przemysław eKaczor

    2015-04-01

    Full Text Available GABA is the major inhibitory neurotransmitter in the adult brain and mechanisms of GABAergic inhibition have been intensely investigated in the past decades. Recent studies provided evidence for an important role of astrocytes in shaping GABAergic currents. One of the most obvious, but yet poorly understood, mechanisms of the cross-talk between GABAergic currents and astrocytes is metabolism including neurotransmitter homeostasis. In particular, how modulation of GABAergic currents by astrocytes depends on key enzymes involved in cellular metabolism remains largely unknown. To address this issue, we have considered two simple models of neuronal cultures: nominally astrocyte-free neuronal culture (NC and neuronal-astrocytic co-cultures (ANCC and miniature Inhibitory Postsynaptic Currents (mIPSCs were recorded in control conditions and in the presence of respective enzyme blockers. We report that enrichment of neuronal culture with astrocytes results in a marked increase in mIPSC frequency. This enhancement of GABAergic activity was accompanied by increased number of GAD65 and vGAT puncta, indicating that at least a part of the frequency enhancement was due to increased number of synaptic contacts. Inhibition of glutamine synthetase (with MSO strongly reduced mIPSC frequency in ANCC but had no effect in NC. Moreover, treatment of ANCC with inhibitor of glycogen phosphorylase (BAYU6751 or with selective inhibitor of astrocytic Krebs cycle,fluoroacetate, resulted in a marked reduction of mIPSC frequency in ANCC having no effect in NC. We conclude that GABAergic synaptic transmission strongly depends on neuron-astrocyte interaction in a manner dependent on key metabolic enzymes as well as on the Krebs cycle.

  13. Data Interactive Publications Revisited

    Science.gov (United States)

    Domenico, B.; Weber, W. J.

    2011-12-01

    A few years back, the authors presented examples of online documents that allowed the reader to interact directly with datasets, but there were limitations that restricted the interaction to specific desktop analysis and display tools that were not generally available to all readers of the documents. Recent advances in web service technology and related standards are making it possible to develop systems for publishing online documents that enable readers to access, analyze, and display the data discussed in the publication from the perspective and in the manner from which the author wants it to be represented. By clicking on embedded links, the reader accesses not only the usual textual information in a publication, but also data residing on a local or remote web server as well as a set of processing tools for analyzing and displaying the data. With the option of having the analysis and display processing provided on the server, there are now a broader set of possibilities on the client side where the reader can interact with the data via a thin web client, a rich desktop application, or a mobile platform "app." The presentation will outline the architecture of data interactive publications along with illustrative examples.

  14. Data Interactive Publications

    Science.gov (United States)

    Domenico, B.; Weber, J.

    2012-04-01

    For some years now, the authors have developed examples of online documents that allowed the reader to interact directly with datasets, but there were limitations that restricted the interaction to specific desktop analysis and display tools that were not generally available to all readers of the documents. Recent advances in web service technology and related standards are making it possible to develop systems for publishing online documents that enable readers to access, analyze, and display the data discussed in the publication from the perspective and in the manner from which the author wants it to be represented. By clicking on embedded links, the reader accesses not only the usual textual information in a publication, but also data residing on a local or remote web server as well as a set of processing tools for analyzing and displaying the data. With the option of having the analysis and display processing provided on the server (or in the cloud), there are now a broader set of possibilities on the client side where the reader can interact with the data via a thin web client, a rich desktop application, or a mobile platform "app." The presentation will outline the architecture of data interactive publications along with illustrative examples.

  15. Public Transparency

    OpenAIRE

    UNCTAD; World Bank

    2018-01-01

    This note provides guidance on the type of information about agricultural investments that investors and governments can make publicly available. Transparency about certain aspects of investments can improve relations between investors and communities, enable external stakeholders to hold investors to commitments, and improve investors’ public image. Although some information should be kep...

  16. Public relations

    International Nuclear Information System (INIS)

    1998-01-01

    Public relations activities continued in a well-proved form of organizing plant visits and Information Centre off - site activities. Bohunice NPPs were visited by the number of 7294 visitors in 1997. A brief account of activities in public relations carried out by the Nuclear power plants Jaslovske Bohunice in 1997 is presented

  17. CONTROL ENVIRONMENT IN KOSOVO PUBLIC INSTITUTIONS

    Directory of Open Access Journals (Sweden)

    Shaqir M. REXHEPI

    2015-06-01

    Full Text Available The study on how is Internal Control System developed in public institutions is of a special importance for modalities, forms and the manner of the application of relevant strategies for the functioning of internal control environment in public institutions. In this paper, there is treated the existing situation of internal control system environment of public finances and its implementation in public sector. For internal control system environment in public finances to function effectively, there should exist a coherent control environment which includes responsibilities for financial management from managers of Publicly Owned Enterprises and with complete functioning of internal audit, which exists in the function of development to add value. In Kosovo public institutions, this framework is offered by legislation and by institutional mechanism for the implementation of legislation according to these parameters.

  18. Behavioral stress alters corticolimbic microglia in a sex- and brain region-specific manner.

    Science.gov (United States)

    Bollinger, Justin L; Collins, Kaitlyn E; Patel, Rushi; Wellman, Cara L

    2017-01-01

    Women are more susceptible to numerous stress-linked psychological disorders (e.g., depression) characterized by dysfunction of corticolimbic brain regions critical for emotion regulation and cognitive function. Although sparsely investigated, a number of studies indicate sex differences in stress effects on neuronal structure, function, and behaviors associated with these regions. We recently demonstrated a basal sex difference in- and differential effects of stress on- microglial activation in medial prefrontal cortex (mPFC). The resident immune cells of the brain, microglia are implicated in synaptic and dendritic plasticity, and cognitive-behavioral function. Here, we examined the effects of acute (3h/day, 1 day) and chronic (3h/day, 10 days) restraint stress on microglial density and morphology, as well as immune factor expression in orbitofrontal cortex (OFC), basolateral amygdala (BLA), and dorsal hippocampus (DHC) in male and female rats. Microglia were visualized, classified based on their morphology, and stereologically counted. Microglia-associated transcripts (CD40, iNOS, Arg1, CX3CL1, CX3CR1, CD200, and CD200R) were assessed in brain punches from each region. Expression of genes linked with cellular stress, neuroimmune state, and neuron-microglia communication varied between unstressed male and female rats in a region-specific manner. In OFC, chronic stress upregulated a wider variety of immune factors in females than in males. Acute stress increased microglia-associated transcripts in BLA in males, whereas chronic stress altered immune factor expression in BLA more broadly in females. In DHC, chronic stress increased immune factor expression in males but not females. Moreover, acute and chronic stress differentially affected microglial morphological activation state in male and female rats across all brain regions investigated. In males, chronic stress altered microglial activation in a pattern consistent with microglial involvement in stress

  19. Magnesium sulfate differentially modulates fetal membrane inflammation in a time-dependent manner.

    Science.gov (United States)

    Cross, Sarah N; Nelson, Rachel A; Potter, Julie A; Norwitz, Errol R; Abrahams, Vikki M

    2018-04-30

    Chorioamnionitis and infection-associated inflammation are major causes of preterm birth. Magnesium sulfate (MgSO 4 ) is widely used in obstetrics as a tocolytic; however, its mechanism of action is unclear. This study sought to investigate how MgSO 4 modulates infection-associated inflammation in fetal membranes (FMs), and whether the response was time dependent. Human FM explants were treated with or without bacterial lipopolysaccharide (LPS); with or without MgSO 4 added either: 1 hour before LPS; at the same time as LPS; 1 hour post-LPS; or 2 hours post-LPS. Explants were also treated with or without viral dsRNA and LPS, alone or in combination; and MgSO 4 added 1 hour post-LPS After 24 hours, supernatants were measured for cytokines/chemokines; and tissue lysates measured for caspase-1 activity. Lipopolysaccharide-induced FM inflammation by upregulating the secretion of a number of inflammatory cytokines/chemokines. Magnesium sulfate administered 1-hour post-LPS inhibited FM secretion of IL-1β, IL-6, G-CSF, RANTES, and TNFα. Magnesium sulfate administered 2 hours post-LPS augmented FM secretion of these factors as well as IL-8, IFNγ, VEGF, GROα and IP-10. Magnesium sulfate delivered 1- hour post-LPS inhibited LPS-induced caspase-1 activity, and inhibited the augmented IL-1β response triggered by combination viral dsRNA and LPS. Magnesium sulfate differentially modulates LPS-induced FM inflammation in a time-dependent manner, in part through its modulation of caspase-1 activity. Thus, the timing of MgSO 4 administration may be critical in optimizing its anti-inflammatory effects in the clinical setting. MgSO 4 might also be useful at preventing FM inflammation triggered by a polymicrobial viral-bacterial infection. © 2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  20. Behavioral stress alters corticolimbic microglia in a sex- and brain region-specific manner

    Science.gov (United States)

    Bollinger, Justin L.; Collins, Kaitlyn E.; Patel, Rushi

    2017-01-01

    Women are more susceptible to numerous stress-linked psychological disorders (e.g., depression) characterized by dysfunction of corticolimbic brain regions critical for emotion regulation and cognitive function. Although sparsely investigated, a number of studies indicate sex differences in stress effects on neuronal structure, function, and behaviors associated with these regions. We recently demonstrated a basal sex difference in- and differential effects of stress on- microglial activation in medial prefrontal cortex (mPFC). The resident immune cells of the brain, microglia are implicated in synaptic and dendritic plasticity, and cognitive-behavioral function. Here, we examined the effects of acute (3h/day, 1 day) and chronic (3h/day, 10 days) restraint stress on microglial density and morphology, as well as immune factor expression in orbitofrontal cortex (OFC), basolateral amygdala (BLA), and dorsal hippocampus (DHC) in male and female rats. Microglia were visualized, classified based on their morphology, and stereologically counted. Microglia-associated transcripts (CD40, iNOS, Arg1, CX3CL1, CX3CR1, CD200, and CD200R) were assessed in brain punches from each region. Expression of genes linked with cellular stress, neuroimmune state, and neuron-microglia communication varied between unstressed male and female rats in a region-specific manner. In OFC, chronic stress upregulated a wider variety of immune factors in females than in males. Acute stress increased microglia-associated transcripts in BLA in males, whereas chronic stress altered immune factor expression in BLA more broadly in females. In DHC, chronic stress increased immune factor expression in males but not females. Moreover, acute and chronic stress differentially affected microglial morphological activation state in male and female rats across all brain regions investigated. In males, chronic stress altered microglial activation in a pattern consistent with microglial involvement in stress

  1. GestuRe and ACtion Exemplar (GRACE) video database: stimuli for research on manners of human locomotion and iconic gestures.

    Science.gov (United States)

    Aussems, Suzanne; Kwok, Natasha; Kita, Sotaro

    2018-06-01

    Human locomotion is a fundamental class of events, and manners of locomotion (e.g., how the limbs are used to achieve a change of location) are commonly encoded in language and gesture. To our knowledge, there is no openly accessible database containing normed human locomotion stimuli. Therefore, we introduce the GestuRe and ACtion Exemplar (GRACE) video database, which contains 676 videos of actors performing novel manners of human locomotion (i.e., moving from one location to another in an unusual manner) and videos of a female actor producing iconic gestures that represent these actions. The usefulness of the database was demonstrated across four norming experiments. First, our database contains clear matches and mismatches between iconic gesture videos and action videos. Second, the male actors and female actors whose action videos matched the gestures in the best possible way, perform the same actions in very similar manners and different actions in highly distinct manners. Third, all the actions in the database are distinct from each other. Fourth, adult native English speakers were unable to describe the 26 different actions concisely, indicating that the actions are unusual. This normed stimuli set is useful for experimental psychologists working in the language, gesture, visual perception, categorization, memory, and other related domains.

  2. Publications, 1978

    International Nuclear Information System (INIS)

    Hilborn, H.S.

    1979-03-01

    This is a compilation of documents that communicate the results of scientific and technical work done at Savannah River. The compilation includes those documents that have been published (research and development reports, journal articles, book chapters, etc.), documents that have been announced in Energy Research Abstracts, and papers that have been presented at technical meetings but have not yet been published or announced. The information was compiled by machine methods to produce bibliographic, subject, and author listings. This report updates the information included in DP-929, Rev. 2, Publications, 1951 through 1971 DP-929-1, Publications, 1972 through 1976, and DP-929-1, Supplement 1, Publications, 1977

  3. Public Airports

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — This data set is a vector point digital data structure that contains the locations of General Public Use Airports in the State of New Mexico. It only contains those...

  4. Public Sociology

    DEFF Research Database (Denmark)

    by the media? Does the choice of public sociology mean the relinquishment of scientific integrity and critical conviction? These questions will also be addressed in this book - together with a host of others related to the topic of public sociology.   The chapters included in this book are all manuscripts......What is the role of sociology in society? How can - and should - sociology contribute with insights relevant and useful to the outside world? Is sociology attuned to accommodate the demands of the wider public and of surrounding society? Who benefits from the knowledge produced and provided...... by sociology? What are the social implications and cultural effects of the knowledge sociology provides and creates? All of these questions, and many others, concern and centre on sociology's relationship to the surrounding society, in short to the ‘public'. All of these questions - and many others...

  5. Semantics-based publication management using RSS and FOAF

    NARCIS (Netherlands)

    Mika, Peter; Klein, Michel; Serban, Radu

    2005-01-01

    Listing references to scientific publications on personal or group homepages is a common practice. Doing this in a consistent and structured manner either requires a lot of discipline or a centralized database. Scientific publication, however, is a distributed activity by nature. We present a

  6. Public meetings

    CERN Multimedia

    Staff Association

    2017-01-01

    Do you have questions about the elections to the Staff Council, 2017 MERIT exercise, EVE and School, LD to IC exercise, CHIS, the Pension Fund… Come get informed and ask your questions at our public meetings. These public meetings are also an opportunity to get the more information on current issues. Benefit from this occasion to get the latest news and to discuss with the representatives of the statutory body that is the Staff Association!

  7. Restructuring Public Higher Education Governance to Succeed in a Highly Competitive Environment. Research & Occasional Paper Series: CSHE.6.15

    Science.gov (United States)

    Hyatt, James A.

    2015-01-01

    Given diminished governmental support, competition from private counterparts, and public demands for access to services, public universities need to respond in an effective manner to take advantage of opportunities and meet the challenges of today's highly competitive environment. A critical factor in meeting these challenges is the manner in…

  8. PAI-1 modulates cell migration in a LRP1-dependent manner via β-catenin and ERK1/2

    DEFF Research Database (Denmark)

    Kozlova, Nina; Jensen, Jan Kristian; Chi, Tabughang Franklin

    2015-01-01

    was proposed to modulate the β-catenin pathway. Therefore, we used wild-type mouse embryonic fibroblasts (MEFs), and MEFs deficient of LRP1 to study PAI-1 as modulator of the β-catenin pathway. We found that PAI-1 influences MEF proliferation and motility in a LRP1-dependent manner and that β...

  9. 26 CFR 1.754-1 - Time and manner of making election to adjust basis of partnership property.

    Science.gov (United States)

    2010-04-01

    ... general. A partnership having an election in effect under this section may revoke such election with the... required to be filed. A partnership which wishes to revoke such an election shall file with the district... 26 Internal Revenue 8 2010-04-01 2010-04-01 false Time and manner of making election to adjust...

  10. Going public

    DEFF Research Database (Denmark)

    Meyer, Gitte; Sandøe, Peter

    2012-01-01

    conduct, contains a tacit maxim of openness which may naturally be extended to cover the public relations of science. Discussing openness as access, accountability, transparency and receptiveness, the argumentation concentrates on the possible prevention of misconduct with respect to, on the one hand......The paper addresses issues of scientific conduct regarding relations between science and the media, relations between scientists and journalists, and attitudes towards the public at large. In the large and increasing body of literature on scientific conduct and misconduct, these issues seem...... deal with ethical public relations issues, guided by a norm or maxim of openness. Drawing on and rethinking the CUDOS codification of the scientific ethos, as it was worked out by Robert K. Merton in 1942, we propose that this, which is echoed in current codifications of norms for good scientific...

  11. Public Sphere

    DEFF Research Database (Denmark)

    Trenz, Hans-Jörg

    2015-01-01

    In modern societies, the public sphere represents the intermediary realm that supports the communication of opinions, the discovery of problems that need to be dealt with collectively, the channeling of these problems through the filter of the media and political institutions, and the realization...... of the collective will of the people in the act of democratic self-government. The concept of the public sphere is used across the fields of media and communication research, cultural studies and the humanities, the history of ideas, legal and constitutional studies as well as democracy studies. Historically......, public spheres have undergone structural transformations that were closely connected to the emergence of different mass media. More recently, they are subject to trends of transnationalization and digitalization in politics and society....

  12. public spaces

    Directory of Open Access Journals (Sweden)

    Elena Grigoryeva

    2013-01-01

    Full Text Available The topic of this issue is PUBLIC SPACES. It is familiar and clear to every citizen. The streets and courtyards as childhood experiences remain with us forever. And these are the places where we come with our parents at weekends, where we meet friends, where we have dates and where we already come for a walk with our children.The history of public spaces is long and captivating. It was the main city squares where the most important events took place in history. The Agoras of Ancient Greece and the Roman Forums, the squares of Vatican, Paris and London, Moscow and Saint Petersburg… Greve, Trafalgar, Senate, Palace, Red, Bolotnaya – behind every name there is life of capitals, countries and nations.Public spaces, their shapes, image and development greatly influence the perception of the city as a whole. Both visitors and inhabitants can see in public spaces not only the visage but the heart, the soul and the mind of the city.Unfortunately, sometimes we have to prove the value of public spaces and defend them from those who consider them nothing but a blank space, nobody’s land destined for barbarous development.What should happen to make citizens perceive public spaces as their own and to make authorities consider development and maintenance of squares and parks their priority task against the  background of increasing competition between cities and the fight for human capital? Lately they more often say about “a high-quality human capital”. And now, when they say “the city should be liveable” they add “for all groups of citizens, including the creative class”.

  13. Public Performance

    Science.gov (United States)

    Krupp, E. C.

    2013-01-01

    America’s first planetaria all opened in the 1930s, and each was the distinctive product of local circumstances. In Los Angeles, the populist sensibilities of Griffith J. Griffith prompted him to value the transformative power of a personal encounter with a telescope, and he quickly embraced the idea of a public observatory with free access to all. Griffith Observatory and its planetarium emerged from that intent. Authenticity, intelligibility, and theatricality were fundamental principles in Griffith’s thinking, and they were transformed into solid and enduring scientific and astronomical values by those who actually guided the Observatory’s design, construction, and programming. That said, the public profile of Griffith Observatory was most defined by its inspired hilltop location, its distinctive, commanding architecture, and its felicitous proximity to Hollywood. The Observatory is theatric in placement and in appearance, and before the Observatory even opened, it was used as a motion picture set. That continuing vocation turned Griffith Observatory into a Hollywood star. Because entertainment industry objectives and resources were part of the Los Angeles landscape, they influenced Observatory programming throughout the Observatory’s history. Public astronomy in Los Angeles has largely been framed by the Observatory’s fundamental nature. It has exhibits, but it is not a museum. It has a planetarium, but it is essentially an observatory. As a public observatory, it is filled with instruments that transform visitors into observers. This role emphasized the importance of personal experience and established the perception of Griffith Observatory as a place for public gathering and shared contact with the cosmos. The Observatory’s close and continuous link with amateur astronomers made amateurs influential partners in the public enterprise. In full accord with Griffith J. Griffith’s original intent, Griffith Observatory has all been about putting

  14. Public acceptance

    International Nuclear Information System (INIS)

    Bucaille, A.

    2006-01-01

    Full text of publication follows: A great deal of determination and professionalism are required when communicating to the public on nuclear energy. Challenging the advantages and adopting an educational tone are, of course, essential. But we have to do much more if we truly want to set people thinking and give nuclear energy its rightful position among the possible energy solutions. This is particularly important in Europe where dissension between countries is on the increase, whereas the US and China, shortly to be joined by India, have clearly decided to invest in nuclear energy. (author)

  15. Public health

    NARCIS (Netherlands)

    Berg, van den A.E.

    2007-01-01

    Agnes van den Berg wrote an essay about human health and nature, establishing that subject as an important policy argument in developing (urban) nature in the Netherlands. She studied the public balance of fear and fascination for nature, summarising benefits on human health. In this chapter, she

  16. Publication Design.

    Science.gov (United States)

    Nelson, Roy Paul

    This book is designed to solve the problem of coordinating art and typography with content in publications. Through text and illustrations, this book suggests ways to make pages and spreads in magazines, newspapers, and books attractive and readable. As a book of techniques, it is directed at potential and practicing art directors, designers, and…

  17. Effect of hydrogel elasticity and ephrinB2-immobilized manner on Runx2 expression of human mesenchymal stem cells.

    Science.gov (United States)

    Toda, Hiroyuki; Yamamoto, Masaya; Uyama, Hiroshi; Tabata, Yasuhiko

    2017-08-01

    The objective of this study is to design the manner of ephrinB2 immobilized onto polyacrylamide (PAAm) hydrogels with varied elasticity and evaluate the effect of hydrogels elasticity and the immobilized manner of ephrinB2 on the Runx2 expression of human mesenchymal stem cells (hMSC). The PAAm hydrogels were prepared by the radical polymerization of acrylamide (AAm), and N,N'-methylenebisacrylamide (BIS). By changing the BIS concentration, the elasticity of PAAm hydrogels changed from 1 to 70kPa. For the bio-specific immobilization of ephrinB2, a chimeric protein of ephrinB2 and Fc domain was immobilized onto protein A-conjugated PAAm hydrogels by making use of the bio-specific interaction between the Fc domain and protein A. When hMSC were cultured on the ephrinB2-immobilized PAAm hydrogels with varied elasticity, the morphology of hMSC was of cuboidal shape on the PAAm hydrogels immobilized with ephrinB2 compared with non-conjugated ones, irrespective of the hydrogels elasticity. The bio-specific immobilization of ephrinB2 enhanced the level of Runx2 expression. The expression level was significantly high for the hydrogels of 3.6 and 5.9kPa elasticity with bio-specific immobilization of ephrinB2 compared with other hydrogels with the same elasticity. The hydrogels showed a significantly down-regulated RhoA activity. It is concluded that the Runx2 expression of hMSC is synergistically influenced by the hydrogels elasticity and their immobilized manner of ephrinB2 immobilized. Differentiation fate of mesenchymal stem cells (MSC) is modified by biochemical and biophysical factors, such as elasticity and signal proteins. However, there are few experiments about combinations of them. In this study, to evaluate the synergistic effect of them on cell properties of MSC, we established to design the manner of Eph signal ligand, ephrinB2, immobilized onto polyacrylamide hydrogels with varied elasticity. The gene expression level of an osteogenic maker, Runx2, was enhanced

  18. The Correlation between Managers’ Delegation of Authority with the Manner of Employee Direction in Hospitals of Qom Province

    OpenAIRE

    Maleki M.R; Nasiri Pour A.A; Amini F; Parham M

    2011-01-01

    Background and Objectives: The destructive effect of centralized management can be found throughout each organization, which is a barrier for delegation of authority and productivity leading to administrative violence increase and compression and frigidity of affairs. With attention to the importance of delegation of authority this research aimed at determining the correlation between the manager's delegation of authority with the manner of employee direction in Qom hospitals, designed for co...

  19. Variation in extracellular matrix genes is associated with weight regain after weight loss in a sex-specific manner

    DEFF Research Database (Denmark)

    Roumans, Nadia J T; Vink, Roel G; Gielen, Marij

    2015-01-01

    The extracellular matrix (ECM) of adipocytes is important for body weight regulation. Here, we investigated whether genetic variation in ECM-related genes is associated with weight regain among participants of the European DiOGenes study. Overweight and obese subjects (n = 469, 310 females, 159 m.......40-5.63). Concluding, variants of ECM genes are associated with weight regain after weight loss in a sex-specific manner....

  20. Exercise training protects against aging-induced mitochondrial fragmentation in mouse skeletal muscle in a PGC-1α dependent manner

    DEFF Research Database (Denmark)

    Halling, Jens Frey; Jørgensen, Stine Ringholm; Olesen, Jesper

    2017-01-01

    Aging is associated with impaired mitochondrial function, whereas exercise training enhances mitochondrial content and function in part through activation of PGC-1α. Mitochondria form dynamic networks regulated by fission and fusion with profound effects on mitochondrial functions, yet the effect...... evidence that exercise training rescues aging-induced mitochondrial fragmentation in skeletal muscle by suppressing mitochondrial fission protein expression in a PGC-1α dependent manner....

  1. Public meetings

    CERN Multimedia

    Staff Association

    2012-01-01

    MARS PENSIONS CONTRACT POLICY GENERAL INFORMATION   PUBLIC MEETINGS COME AND BE INFORMED! Public meetings Monday 15 Oct. 2 pm Amphi IT, 31-3-004 Meyrin Wednesday 17 Oct. 10 am Amphi BE, 864-1-D02 Prévessin Thursday 18 Oct. 10 am Salle du Conseil/ Council Chamber 503-1-001 Meyrin Thursday 18 Oct. 2 pm Filtration Plant, 222-R-001(in English) Meyrin   Overview of the topics to be discussed Recognition of Merit – MARS Outcome of last exercise 2007 to 2012 : lessons learned Pension Fund Capital preservation policy : what is it ? Contract policy LC2IC statistics SA proposal General information CVI 2013 Voluntary programmes (PRP, SLS)  

  2. Publications, 1977

    International Nuclear Information System (INIS)

    Hilborn, H.S.

    1978-02-01

    A compilation of documents that communicate the results of scientific and technical work done at Savannah River is given. The compilation includes those documents that have been published (research and development reports, journal articles, book chapters, etc.), documents that have been announced in Energy Research Abstracts, and papers that have been presented at technical meetings but have not yet been published or announced. The information was compiled by machine methods to produce bibliographic, subject, and author listings. This report updates the information included in DP-929, Rev. 2, Publications, 1951 through 1971 and DP-929-1, Publications, 1972 through 1976. The bibliographic listing is arranged alphabetically by the first-mentioned author of each document. Author and subject indexes are provided

  3. Public relations

    International Nuclear Information System (INIS)

    1996-01-01

    At Nuclear Regulatory Authority of the Slovak Republic (NRA SR), the public relations belongs to the secretariat of the Chairman, and are a part of the policy of carefully planned and purposeful efforts to establish mutual relations between the authority and the public. A spokesmen of NRA SR is in charge of the public relations. The spokesman is ready, without a useless filibuster and based on a particular requirement, to inform governmental bodies, other national bodies and organizations, embassies and international organizations, the public and news media in case of an event at a nuclear installation. To provide for communications activities, NRA SR constructed and opened the Information centre with a particular equipment in autumn 1995, that has already started communications with some dailies, broadcasting, television and Press Agency SR. It has been envisaged that there will be press conferences held in the Information centre a few times a year, or NRA SR senior staff may be interviewed here on extraordinary events at NPPs, or on some other important occasions in NRA SR. In 1995, NRA Sr issued the Annual report in a few variants - each suitable for different use - on NRA SR activities and nuclear safety of Slovak nuclear power plants as of 1994. The NRA SR's Bulletin has started to be published with periodicity of 3 times a year, focusing on NRA SR activities both in Slovakia and abroad. NRA SR Information centre provides foreign visitors with independent propagation and information materials about the issue of nuclear safety enhancement at operational Slovak NPPs. Furthermore, the Information centre provides both the NRA SR's residences in Bratislava and Trnava with daily press monitoring of topical news

  4. How Public Is Public Administration? A Constitutional Approach of Publicness

    Science.gov (United States)

    Ringeling, Arthur

    2015-01-01

    Both in Public Administration and in practice, there is a loss of the concept of public. A view became dominant in which markets were superior to governments and public to private. Not only did the esteem of the public sphere diminish, but also its significance in our reasoning and teaching. It became less clear what the public sphere stood for.…

  5. Public relations as public information

    Energy Technology Data Exchange (ETDEWEB)

    Coninx, C.

    1983-01-01

    To the man in the street, the topic of nuclear energy on the one hand is a highly emotional subject incorporating dangers hard to assess. However, on the other hand, there is in most cases little inclination to concern oneself with problems of energy economy. Public relations activities therefore frequently must motivate their adresses to accept information before they can actually inform. In this connection, the informing party must appear trustworthy and avoid that a teacher : pupil relationship is created. In addition, the changes in the socio-political environment must be taken into account. Within the industries and organizations engaged in the nuclear field there is the problem that the effects and the impact of public relations work can hardly be gaged by individually, which greatly hampers quantification of the work actually done.

  6. Public relations

    International Nuclear Information System (INIS)

    1998-01-01

    The Nuclear Regulatory Authority of the Slovak Republic (NRA SR) laid the foundation of a policy of keeping the public informed about the activities and the safety of nuclear installations in the SR already in its origins by opening the NRA Information Centre. The NRA SR issues the Bulletin on NRA SR for domestic and foreign use, the bilingual Annual report on NRA activities for domestic and foreign public - detailed report for the specialists and a version for the Slovak Republic state authorities. On the occasion of the anniversary of the IAEA a special publication on the IAEA was prepared. Forty-nine contributions on NRA national and foreign activities were trans-missed to the Press Agency of the SR (TASR) over the course of 1997. In the journal Safety of Nuclear Energy the column 'Information' periodically carries the briefs on NRA activities. Four essential articles on NRA mission and responsibility in the are of peaceful uses of atomic energy were published in the Newsletter of the Slovak Information Agency (SIA). Contributions on NRA activities and international co-operation are periodically put out in the 'Slovenske elektrarne' Newsletter, the Slovak Nuclear Society Bulletin. Report on the Safety of NPPs in the Slovak Republic is annually published in the Journal European Nuclear Society - Nuclear Europe Worldscan. Some articles on NRA activities were published in the world information agency NucNet. The NRA issued in 1997 four editions of the internal Bulletin on the NRA's national and foreign activities and personnel. Three press conferences were held and six events were arranged in radio broadcasting in 1997

  7. Public meetings

    CERN Multimedia

    Staff Association

    2014-01-01

      Public meetings : Come and talk about your future employment conditions !   The Staff Association will come and present the results of our survey on the 2015 five-yearly review. Following the survey, the topics discussed, will be contract policy, recognition of merit (MARS), working time arrangements and family policy. After each meeting and around a cup of coffee or tea you will be able to continue the discussions. Do not hesitate to join us, the five-yearly review, it is with YOU!

  8. Public meetings

    CERN Multimedia

    Staff Association

    2014-01-01

      MARS 2015 FIVE YEARLY REVIEW CONTRACT POLICY PENSION FUND GENERAL INFORMATION   COME AND BE INFORMED! PUBLIC MEETINGS Friday 3rd October at 10 am Amphi BE, 864-1-D02 Prévessin Friday 3rd October at 2 pm Salle du Conseil / Council Chamber, 503-1-001 (in English) Meyrin Monday 6th October at 10 am Kjell Johnsen Auditorium, 30-7-018 Meyrin Monday 6th October at 2 pm Salle du Conseil / Council Chamber, 503-1-001 Meyrin  

  9. Managing Public Accountability : How Public Managers Manage Public Accountability

    NARCIS (Netherlands)

    Schillemans, Thomas

    2015-01-01

    Accountability is of growing importance in contemporary governance. The academic literature on public accountability is fraught with concerned analyses, suggesting that accountability is a problematic issue for public managers. This article investigates how public managers experience accountability

  10. Public relations

    International Nuclear Information System (INIS)

    1997-01-01

    Nuclear Regulatory Authority of the Slovak Republic (NRA SR) started building up a broad information publicity conception resulting in NRA SR Information Centre establishment in 1995. The Centre provides information on the NRA SR activities. The Authority's speaker provides information about NRA SR domestic and foreign activities for TASR, newspapers, Slovak radio and television. Information on nuclear energy facilities situation is given to state authorities, organizations as well as to domestic and foreign mass-media on request. 41 press releases, 3 radio interviews and 5 television records providing information on some important NRA SR events was worked out in 1996. 4 basic NRA SR document have been presented by the Slovak Information Agency, consequently 3 press-conferences were held. Concerning some less frequent communication activities, NRA SR can present the NRA SR annual report in three versions (for the Slovak Government, for public and detailed report for experts). NRA SR and State Authority for Nuclear Safety of the Czech Republic issue together Nuclear Energy Safety News every two months. NRA SR activities Bulletin started issuing in 1996 3 to 4 volumes per year. Newspaper, radio and television news monitoring is performed for Bratislava and Trnava offices providing interesting papers or important events records and photos of NRA SR visits. Practical experience of the information activities performance in connection with the Authority mission and liability was obtained last year. Methodical and organizational guidelines to adjust the state regulatory authority activities in this field have been prepared

  11. Vico and Literary Mannerism

    DEFF Research Database (Denmark)

    Catana, Leo

    Reviews: Scott Samuelson, New Vico studies, vol. 18 (2000), pp. 111-115; Maurizio Martirano, Sesto contribuito alla bibliografia vichiana (1996-2000); Alfredo Guida Editore: ?, (2002), p. 70 (Studi vichiani, vol. 37)...

  12. Synthesis and structure of a 2D Zn complex with mixed ligands stacked in offset ABAB manner

    Energy Technology Data Exchange (ETDEWEB)

    Qin, Ling, E-mail: qinling0924013@163.com; Wang, Yan-Qing; Ni, Gang [Hefei University of Technology, Department of chemical engineering and food processing, Xuancheng Campus (China)

    2016-07-15

    The title complex, ([Zn(ODIB){sub 1/2}(bpdc)]·2DMF){sub n} was prepared under hydrothermal conditions (dimethylformamide and water) based on two ligands, namely, 1,1′-oxy-bis[3,5-diimidazolyl-benzene] (ODIB) and biphenyldicarboxylic acid (H{sub 2}bpdc). ODIB ligands link Zn cations to give layers in crystal. bpdc{sup 2–} anions coordinate to Zn atoms, however, their introduction does not increase the dimension of the structure. Each layer is partially passes through the adjacent layers in the offset ABAB manner.

  13. Public meeting

    CERN Multimedia

    HR Department

    2010-01-01

    Dear Colleagues, I am pleased to invite you to a public meeting which will be held on Thursday 11 November 2010 at 2:30 p.m., in the Main Auditorium (welcome coffee from 2 p.m.) In this meeting Sigurd Lettow, Director for Administration and General Infrastructure will present the Management’s proposals towards restoring full funding of the Pension Fund. The meeting will follow discussions which took place with the Staff Association, at the Standing Concertation Committee (CCP) of 1 November 2010 and will be held with the Members States, at the Tripartite Employment Conditions Forum (TREF) of 4 November 2010. You will be able to attend this presentation in the Main Auditorium or via the webcast. The Management will also be available to reply to your questions on this subject. Best regards, Anne-Sylvie Catherin

  14. Public meetings

    CERN Multimedia

    Staff Association

    2013-01-01

    MARS SURVEY 5YR 2015 GENERAL INFORMATION ELECTIONS 2013   COME AND BE INFORMED! Public meetings Tuesday 1st Oct. 10 am Amphi IT, 31-3-004 Meyrin Tuesday 1st Oct. 2 pm Council Chamber, 503-1-001 Meyrin Friday 4 Oct. 10 am Amphi BE, 864-1-D02 Prévessin Monday 7 Oct. 2 pm Council Chamber, 503-1-001 (in English) Meyrin Tuesday 8 Oct. 10 am Amphi Kjell Johnsen, 30-7-018 Meyrin   Overview of the topics to be discussed Recognition of Merit – MARS Outcome of last exercise 2007 to 2013: lessons learned Survey: five-yearly review, give us your opinion General information CVI 2014 Voluntary programmes (PRP, SLS) Elections 2013 Renewal of the Staff Council 2014 - 2015  

  15. Public acceptance and public relations

    International Nuclear Information System (INIS)

    Tanaka, Yasumasa

    1977-01-01

    A set of problems are discussed, which must be studied before the public relations are dealt with. Firstly, the trade-off between energy and health must be considered. There were several ages in which the consideration on health took preference to the energy requirement in the past. For example, the use of coal in London was prohibited by the King's proclamation in 1,306. Secondly, the selection for the acceptance of atomic power development and utilization is based on the subjective susceptibility psychologically, and cannot be concluded only by the logical reasoning. Thirdly, the strict definition of ''national consensus'' is necessary. That is, whether does it mean pleviscite or mere mood. Fourthly, whether the atomic energy is free from the danger or death biologically or not. Fifthly, is there any method for discriminating the persons who accept atomic power from the persons who do not socially. Although the probability of death caused by atomic accidents is very small (one three hundred millionth a year), many peoples hate atomic power and oppose to the construction of nuclear power plants. Four reasons for this are considered: (1) social diffusion of innovation, (2) nuclear allergy, (3) shortage of the conception of risk-benefit, and (4) heterogeneity of the public. According to the investigation of the relationship between electric power and livelihood, carried out by the policy and science research institute in Tokyo, the highly subjective decision for the acceptance of atomic power is independent of the objective knowledge on atomic power. (Iwakiri, K.)

  16. Effect of chest wall radiotherapy in different manners using tissue equivalent bolus on skin and lung of cavia cobayas

    International Nuclear Information System (INIS)

    Huang Wei; Qu Yaqin; Song Xiangfu; Liu Shixin; Jia Xiaojing; Guo He; Yang Lei

    2009-01-01

    Objective: To probe the influence of electron beam radiotherapy in different manners using different tissue equivalent boluses on skin and lung. Methods: Adult female cavia cobayas were randomly divided into four groups as control group, half-time with bolus group, half-time with bolus group and without bolus group. Acute-irradiation animal models were established using electron beam in different manners with or without 0.5 cm tissue equivalent bolus. Pathological changes in lung, hair vesicle and fibroblast cell count were analyzed 40 clays after irradiation. Results: The radiation dermatitis in the group with bolus was slighter than that of the group without bolus, but the radiation pneumonia was reverse. With bolus, the radiation dermatitis of haft-time group was slighter than that of full-time group. The injury repair of half-time group was more active than full-time group. Conclusions: The treatment of haft-time bolus could protect lung without serious skin complications. (authors)

  17. O-GlcNAcylation modulates PKA-CREB signaling in a manner specific to PKA catalytic subunit isoforms.

    Science.gov (United States)

    Jin, Nana; Ma, Denglei; Gu, Jianlan; Shi, Jianhua; Xu, Xiaotao; Iqbal, Khalid; Gong, Cheng-Xin; Liu, Fei; Chu, Dandan

    2018-02-26

    O-GlcNAcylation is a post-translational modification of proteins. Protein kinase A (PKA)-cAMP response element binding protein (CREB) signaling plays critical roles in multiple biological processes. Isoforms α and β of PKA catalytic subunit (PKAc) and CREB are modified by O-GlcNAcylation. In the present study, we determined the role of O-GlcNAcylation in PKAc isoform-specific CREB signaling. We found that up-regulation of O-GlcNAcylation enhanced CREB phosphorylation, but suppressed CREB expression in exogenous PKAc isoform-unspecific manner. PKAc isoforms affected exogenous expression of OGT or OGA and protein O-GlcNAcylation differently. Up-regulation of O-GlcNAcylation did not significantly affect net PKAcα-CREB signaling, but enhanced PKAcβ-CREB signaling. The role of O-GlcNAcylation in PKA-CREB signaling was desensitized by insulin treatment. This study suggests a role of O-GlcNAcylation in PKA-CREB signaling by affecting phosphorylation of CREB in a PKAc isoform-specific manner. Copyright © 2018 Elsevier Inc. All rights reserved.

  18. Choosing a public-spirited leader

    DEFF Research Database (Denmark)

    Markussen, Thomas; Tyran, Jean-robert

    2017-01-01

    In this experiment, voters select a leader who can either act in the public interest, i.e. make efficient and equitable policy choices, or act in a corrupt way, i.e. use public funds for private gain. Voters can observe candidates⿿ pro-social behavior and their score in a cognitive ability test...... prior to the election, and this fact is known to candidates. Therefore, self-interested candidates have incentives to act in a pro-social manner, i.e. to pretend to be public-spirited leaders. We find that both truly pro-social and egoistic leaders co-exist, but that political selection is ineffective...... in choosing public-spirited leaders. The main reason is that egoistic candidates strategically pretend to be pro-social to increase their chances of winning the election....

  19. Public History

    Directory of Open Access Journals (Sweden)

    Marta Gouveia de Oliveira Rovai

    2017-04-01

    Full Text Available Este artigo tem como proposta apresentar o conceito e as práticas de História Pública como um novo posicionamento da ciência histórica em diálogo com profissionais da comunicação, no sentido de produzir e divulgar as experiências humanas. Para isso, discute-se a origem do conceito de História Pública e as diferentes formas de educação histórica que a utilização das novas tecnologias podem proporcionar (dentre elas a internet. Nesse sentido, convida-se o leitor para a reflexão sobre as possibilidades de publicização e de democratização do conhecimento histórico e da cultura, ampliando-se a oportunidade de produção, de divulgação e de acesso do público a diferentes formas experiências no tempo. O artigo também intenciona chamar atenção dos profissionais que lidam com a História e com a Comunicação para os perigos de produções exclusivamente submetidas ao mercado que transformam a popularização da História no reforço de estigmas culturais.   PALAVRAS-CHAVE: História Pública; Educação histórica e Comunicação; democratização e estigmatização.     ABSTRACT This article aims to present the concept and practices of Public History as a new positioning of historical science in dialogue with communication professionals, in the sense of producing and disseminating human experiences. For this, the origin of the concept of Public History and the different forms of historical education that the use of the new technologies can provide (among them the Internet is discussed. In this sense, the reader is invited to reflect on the possibilities of publicizing and democratizing historical knowledge and culture, expanding the opportunity for production, dissemination and public access to different forms of experience in time. The article also intends to draw attention from professionals dealing with History and Communication to the dangers of exclusively commercialized productions that transform the popularization

  20. Public Relations Strategies for Scholastic Publication Staffs.

    Science.gov (United States)

    Konkle, Bruce E.

    2000-01-01

    Discusses the importance to scholastic publications staffs of four public relations strategies: meticulous research, systematic planning, strengthening communication efforts, and evaluation. Notes internal and external factors crucial to good public relations. Lists activities to consider. (SR)

  1. Public Entrepreneurship

    DEFF Research Database (Denmark)

    Hjorth, Daniel

    2013-01-01

    In this paper we want to affirm the desiring-social-change that we find in practices presently represented by theorists and policy-makers as examples of ‘social entrepreneurship’ (SE). We do this as an attempt to intensify the presence of the social and sociality in today's discourse on the entre......In this paper we want to affirm the desiring-social-change that we find in practices presently represented by theorists and policy-makers as examples of ‘social entrepreneurship’ (SE). We do this as an attempt to intensify the presence of the social and sociality in today's discourse...... on the entrepreneurship–society relationship. SE, as all entrepreneurship practices, operates by social and economic forces (limiting ourselves to those here), and generates social and economic outcomes (amongst others). Its second half, however, dominates the concept of SE, and our analysis seek to remedy this imbalance...... by focusing on the social productivity of entrepreneurship, on entrepreneurship desiring social change. We suggest ‘public entrepreneurship’ might grasp this as a more balanced concept that will also support a more precise analysis of the entrepreneurship–society relationship....

  2. Public meeting

    CERN Multimedia

    Staff Association

    2015-01-01

    Last Monday at 9 a.m. the Council Chamber was full, with several people standing, for the public meeting of the Staff Association. Simultaneously, many of our colleagues followed the presentations in the Amphitheatre in Prévessin. We would like to thank all of you for the interest you have shown and for your feedback. In the introduction we explained how the Staff Association represents the staff in its discussions with Management and Member States, and how the staff itself defined, by its participation in the 2013 staff survey, the priority assigned to various points related to the employment conditions. The position of the Staff Association regarding the new contract policy, to be implemented as of 31 March 2015 after approval by Council, was stated. Then, in the framework of the 2015 five-yearly review, the general approach that we would like to see for the new career structure, was explained. Concerning diversity, based on what we know about the situation in other international organiza...

  3. Budget Brief: 2015 Proposed Budget Milwaukee Public Schools

    Science.gov (United States)

    Allen, Vanessa; Chapman, Anne; Henken, Rob

    2014-01-01

    In this report, the authors provide a detailed analysis of the major changes in revenue and expenditures in the Milwaukee Public Schools (MPS) 2015 proposed budget, and the manner in which MPS has responded to recent legislative changes and turbulent workforce challenges. The objective is to provide an independent assessment of the district's…

  4. Embodiments of Public Pedagogy: The Art of Soulful Resistance

    Science.gov (United States)

    Darder, Antonia

    2011-01-01

    This article provides a space to explore, through artistic representations and the words of artists themselves, the manner in which politically engaged artists use their visual art, poetry, music, dance, and theatre performances as an effective tool for public pedagogy. In turn, these artists provide those who enter into their cultural production…

  5. An audit of the toxicology findings in 555 medico-legal autopsies finds manner of death changed in 5 cases.

    Science.gov (United States)

    Langlois, Neil E I; Gilbert, John D; Heath, Karen J; Winskog, Calle; Kostakis, Chris

    2013-03-01

    An audit of toxicological analysis in Coronial autopsies performed at Forensic Science South Australia was conducted on the cases of three pathologists. Toxicological analysis had been performed in 555 (68 %) from a total of 815 autopsies. It was found that the proffered manner of death was changed from the provisional report (provided immediately after the post-mortem examination) in five cases (just under 1 %) as a consequence of the toxicological findings. This is a limited study as it is retrospective, not all cases had toxicological analysis and the findings are constrained by the range of the substances that could be detected. Nonetheless, the audit supports the application of toxicological analysis in medico-legal death investigation and suggests that an inclusive policy should be adopted.

  6. The Role of Perception in the Typology of Geminate Consonants: Effects of Manner of Articulation, Segmental Environment, Position, and Stress.

    Science.gov (United States)

    Dmitrieva, Olga

    2018-03-01

    The present study seeks to answer the question of whether consonant duration is perceived differently across consonants of different manners of articulation and in different contextual environments and whether such differences may be related to the typology of geminates. The results of the cross-linguistic identification experiment suggest higher perceptual acuity in labeling short and long consonants in sonorants than in obstruents. Duration categories were also more consistently and clearly labeled in the intervocalic than in the preconsonantal environment, in the word-initial than in the word-final position, and after stressed vowels than between unstressed vowels. These perceptual asymmetries are in line with some typological tendencies, such as the cross-linguistic preference for intervocalic and post-stress geminates, but contradict other proposed cross-linguistic patterns, such as the preference for obstruent geminates and the abundance of word-final geminates.

  7. GSK3β is involved in the relief of mitochondria pausing in a Tau-dependent manner.

    Directory of Open Access Journals (Sweden)

    María Llorens-Martín

    Full Text Available Mitochondrial trafficking deficits have been implicated in the pathogenesis of several neurological diseases, including Alzheimer's disease (AD. The Ser/Thre kinase GSK3β is believed to play a fundamental role in AD pathogenesis. Given that GSK3β substrates include Tau protein, here we studied the impact of GSK3β on mitochondrial trafficking and its dependence on Tau protein. Overexpression of GSK3β in neurons resulted in an increase in motile mitochondria, whereas a decrease in the activity of this kinase produced an increase in mitochondria pausing. These effects were dependent on Tau proteins, as Tau (-/- neurons did not respond to distinct GSK3β levels. Furthermore, differences in GSK3β expression did not affect other parameters like mitochondria velocity or mitochondria run length. We conclude that GSK3B activity regulates mitochondrial axonal trafficking largely in a Tau-dependent manner.

  8. Demonstration of Data Interactive Publications

    Science.gov (United States)

    Domenico, B.; Weber, J.

    2012-04-01

    This is a demonstration version of the talk given in session ESSI2.4 "Full lifecycle of data." For some years now, the authors have developed examples of online documents that allowed the reader to interact directly with datasets, but there were limitations that restricted the interaction to specific desktop analysis and display tools that were not generally available to all readers of the documents. Recent advances in web service technology and related standards are making it possible to develop systems for publishing online documents that enable readers to access, analyze, and display the data discussed in the publication from the perspective and in the manner from which the author wants it to be represented. By clicking on embedded links, the reader accesses not only the usual textual information in a publication, but also data residing on a local or remote web server as well as a set of processing tools for analyzing and displaying the data. With the option of having the analysis and display processing provided on the server (or in the cloud), there are now a broader set of possibilities on the client side where the reader can interact with the data via a thin web client, a rich desktop application, or a mobile platform "app." The presentation will outline the architecture of data interactive publications along with illustrative examples.

  9. The Correlation between Managers’ Delegation of Authority with the Manner of Employee Direction in Hospitals of Qom Province

    Directory of Open Access Journals (Sweden)

    Maleki M.R

    2011-08-01

    Full Text Available Background and Objectives: The destructive effect of centralized management can be found throughout each organization, which is a barrier for delegation of authority and productivity leading to administrative violence increase and compression and frigidity of affairs. With attention to the importance of delegation of authority this research aimed at determining the correlation between the manager's delegation of authority with the manner of employee direction in Qom hospitals, designed for contributing to improvement of managers’ performance in hospitals.Methods: This correlational and cross-sectional research, was carried out on all the employees under the control of managers and chiefs of Qom province hospitals (N=2167. Sampling was done by cluster sampling method through the use of Cokran sampling based on Morgan and kerjsi chart. 998 samples with the confidence level of %95 and permitted errors of 0.05 were randomly selected. Data were collected via questionnaires which were answered by self-report method. The data were then analyzed by the Pearson correlation coefficient, variance's test F analyses, multiple variant regression and T-test.Results: The mean score of the manager's non-delegation of authority was (32.4. Among the indicators of the direction, there was a significant relationship between the motivation (mean= 61.44 and organizational communication (49.39, also there was a significant relationship between the managers’ delegation of authority and the manner of their employees direction (non-delegation of authority R=-0.13. Conclusion: Due to the meaningful and direct relationship between most of direction variables with the managers’ delegation of authority, increasing the delegation of authority and deconcentration can lead to an increase in employee motivation and (vertical and formal communication and improved performance of affairs.

  10. Adiponectin receptor 2 is regulated by nutritional status, leptin and pregnancy in a tissue-specific manner.

    Science.gov (United States)

    González, Carmen Ruth; Caminos, Jorge Eduardo; Gallego, Rosalía; Tovar, Sulay; Vázquez, María Jesús; Garcés, María Fernanda; Lopez, Miguel; García-Caballero, Tomás; Tena-Sempere, Manuel; Nogueiras, Rubén; Diéguez, Carlos

    2010-01-12

    The aim of the present work was to study the regulation of circulating adiponectin levels and the expression of adiponectin receptor 2 (Adipo-R2) in several rat tissues in relation to fasting, leptin challenge, pregnancy, and chronic undernutrition. Using real-time PCR, we found Adipo-R2 mRNA expression in the liver, stomach, white and brown adipose tissues (WAT and BAT) of adult rats. Immunohistochemical studies confirmed protein expression in the same tissues. Adipo-R2 mRNA levels were decreased in liver after fasting, with no changes in the other tissues. Leptin decreased Adipo-R2 expression in liver and stomach, but increased its expression in WAT and BAT. Chronic caloric restriction in normal rats increased Adipo-R2 gene expression in stomach, while it decreased hepatic Adipo-R2 levels in pregnant rats. Using radioimmunoassay, we found that plasma adiponectin levels were diminished by fasting and leptin. Conversely, circulating adiponectin was increased in food-restricted rats, whereas its levels decreased in food-restricted pregnant rats by the end of gestation. In conclusion our findings provide the first evidence that (a) Adipo-R2 mRNA is regulated in a tissue-specific manner by fasting, but leptin is not responsible for those changes; (b) chronic caloric restriction in normal and pregnant rats also regulate Adipo-R2 mRNA in a tissue-specific manner; and (c) Adipo-R2 mRNA does not show a clear correlation with plasma adiponectin levels.

  11. Molecular Stress-inducing Compounds Increase Osteoclast Formation in a Heat Shock Factor 1 Protein-dependent Manner*

    Science.gov (United States)

    Chai, Ryan C.; Kouspou, Michelle M.; Lang, Benjamin J.; Nguyen, Chau H.; van der Kraan, A. Gabrielle J.; Vieusseux, Jessica L.; Lim, Reece C.; Gillespie, Matthew T.; Benjamin, Ivor J.; Quinn, Julian M. W.; Price, John T.

    2014-01-01

    Many anticancer therapeutic agents cause bone loss, which increases the risk of fractures that severely reduce quality of life. Thus, in drug development, it is critical to identify and understand such effects. Anticancer therapeutic and HSP90 inhibitor 17-(allylamino)-17-demethoxygeldanamycin (17-AAG) causes bone loss by increasing osteoclast formation, but the mechanism underlying this is not understood. 17-AAG activates heat shock factor 1 (Hsf1), the master transcriptional regulator of heat shock/cell stress responses, which may be involved in this negative action of 17-AAG upon bone. Using mouse bone marrow and RAW264.7 osteoclast differentiation models we found that HSP90 inhibitors that induced a heat shock response also enhanced osteoclast formation, whereas HSP90 inhibitors that did not (including coumermycin A1 and novobiocin) did not affect osteoclast formation. Pharmacological inhibition or shRNAmir knockdown of Hsf1 in RAW264.7 cells as well as the use of Hsf1 null mouse bone marrow cells demonstrated that 17-AAG-enhanced osteoclast formation was Hsf1-dependent. Moreover, ectopic overexpression of Hsf1 enhanced 17-AAG effects upon osteoclast formation. Consistent with these findings, protein levels of the essential osteoclast transcription factor microphthalmia-associated transcription factor were increased by 17-AAG in an Hsf1-dependent manner. In addition to HSP90 inhibitors, we also identified that other agents that induced cellular stress, such as ethanol, doxorubicin, and methotrexate, also directly increased osteoclast formation, potentially in an Hsf1-dependent manner. These results, therefore, indicate that cellular stress can enhance osteoclast differentiation via Hsf1-dependent mechanisms and may significantly contribute to pathological and therapeutic related bone loss. PMID:24692538

  12. Molecular stress-inducing compounds increase osteoclast formation in a heat shock factor 1 protein-dependent manner.

    Science.gov (United States)

    Chai, Ryan C; Kouspou, Michelle M; Lang, Benjamin J; Nguyen, Chau H; van der Kraan, A Gabrielle J; Vieusseux, Jessica L; Lim, Reece C; Gillespie, Matthew T; Benjamin, Ivor J; Quinn, Julian M W; Price, John T

    2014-05-09

    Many anticancer therapeutic agents cause bone loss, which increases the risk of fractures that severely reduce quality of life. Thus, in drug development, it is critical to identify and understand such effects. Anticancer therapeutic and HSP90 inhibitor 17-(allylamino)-17-demethoxygeldanamycin (17-AAG) causes bone loss by increasing osteoclast formation, but the mechanism underlying this is not understood. 17-AAG activates heat shock factor 1 (Hsf1), the master transcriptional regulator of heat shock/cell stress responses, which may be involved in this negative action of 17-AAG upon bone. Using mouse bone marrow and RAW264.7 osteoclast differentiation models we found that HSP90 inhibitors that induced a heat shock response also enhanced osteoclast formation, whereas HSP90 inhibitors that did not (including coumermycin A1 and novobiocin) did not affect osteoclast formation. Pharmacological inhibition or shRNAmir knockdown of Hsf1 in RAW264.7 cells as well as the use of Hsf1 null mouse bone marrow cells demonstrated that 17-AAG-enhanced osteoclast formation was Hsf1-dependent. Moreover, ectopic overexpression of Hsf1 enhanced 17-AAG effects upon osteoclast formation. Consistent with these findings, protein levels of the essential osteoclast transcription factor microphthalmia-associated transcription factor were increased by 17-AAG in an Hsf1-dependent manner. In addition to HSP90 inhibitors, we also identified that other agents that induced cellular stress, such as ethanol, doxorubicin, and methotrexate, also directly increased osteoclast formation, potentially in an Hsf1-dependent manner. These results, therefore, indicate that cellular stress can enhance osteoclast differentiation via Hsf1-dependent mechanisms and may significantly contribute to pathological and therapeutic related bone loss.

  13. Fall of the public teacher

    DEFF Research Database (Denmark)

    Johansen, Martin Blok

    2015-01-01

    This article reports on a case study conducted in a Danish municipality with 22 teachers as participants, in the context of a project focused on well-being and key educational competencies. The final aim was that of discovering how their ideal of the good teacher was verbalised. What...... is it that they thematise? How are thematisations created and maintained? The study is theoretically based on Richard Sennett’s notions on the private–public divide and Michel Foucault’s definitions of power and discourse. It focuses on the manners in which Danish teachers articulate a discourse in which words and phrases......, the article provides (a) a discussion about the ontology of intimacy; (b) a threefold typology of intimacy, namely confessional, emotional and relational, a categorisation that stems from the teachers’ statements and exchanges when discussing good performance; and (c) a reflection on the concept of mode...

  14. Public values in public-private partnerships

    NARCIS (Netherlands)

    Reynaers, A.; de Graaf, G.

    2014-01-01

    Although public-private partnerships (PPPs) are often evaluated in terms of efficiency, their impact on public values is often neglected. In order to find out what we know about the public values-PPPs relation, this article reviews public administration literature and describes two opposite

  15. Lovers and City A Study about Youth and Uses of Public Spaces

    OpenAIRE

    Fardin Alikhah; Masomeh Shadmanfaat

    2016-01-01

    There has been a huge concern about the manner of confrontations of different social groups with urban public spaces within urban scholars. Among these social groups, young people have been particularly important. Because they have a tangible presence in the city's public spaces and social life of the city are affected by their presence. This paper examines the uses of public spaces by young people and will pay special attention to the role of social control on use of public spaces. Paper foc...

  16. Chapter 12. Public information

    International Nuclear Information System (INIS)

    2001-01-01

    The Nuclear Regulatory Authority of the Slovak Republic (UJD) is around to serve the public. This is a regulatory institution that furnishes truthful and independent information on nuclear safety of nuclear installations, including information on management of radioactive wastes, spent fuel, nuclear materials, control and record-keeping thereof, as well as information on other fuel cycle phases. UJD seeks to provide information in an orderly, accurate and comprehensible manner in such a way that it cannot be disclaimed, as the public has the right to know the truth. In formulating information, regularity, consistency and openness are of high importance, because information presented cannot serve a controversy. UJD furnishes information independently and separately from that issued by nuclear power plant operators. As a central administration authority, UJD provides on request, within the field of its responsibility, in particular the information on safety of nuclear energy installations independently from nuclear programme implementers, allowing the public and the media to check up the data and information thereon. A significant element of the information is the demonstration that the area of nuclear energy uses in the Slovak Republic has its binding rules and the compliance therewith is controlled by the state through an independent institution - UJD. As early as in 1995 the foundations laid were at UJD for the concept for broad public information on UJD activity and on safety of nuclear installations by opening UJD Information Centre. This Centre provides communication with the public and the media, thereby contributing to fashioning among the public a favourable image of the independent state supervision over nuclear safety. The Information Centre was extended in 2000. In 2000, 76 contributions on domestic and foreign UJD activities were sent in to Slovak press agencies, dailies and electronic media. UJD is together with the SUJB the publisher of the journal

  17. Inactive Publics: The Forgotten Publics in Public Relations.

    Science.gov (United States)

    Hallahan, Kirk

    2000-01-01

    Notes that recent public relations theory has largely ignored inactive publics, stakeholder groups that demonstrate low levels of knowledge and involvement in the organization or its products, services, candidates, or causes, but are important to an organization. Examines the nature of inactive publics and proposes a model that locates inactive…

  18. The Correlation between Managers’ Delegation of Authority with the Manner of Employee Direction in Hospitals of Qom Province

    Directory of Open Access Journals (Sweden)

    M.R Maleki

    2012-05-01

    Full Text Available

    Background and Objectives: The destructive effect of centralized management can be found throughout each organization, which is a barrier for delegation of authority and productivity leading to administrative violence increase and compression and frigidity of affairs. With attention to the importance of delegation of authority this research aimed at determining the correlation between the manager's delegation of authority with the manner of employee direction in Qom hospitals, designed for contributing to improvement of managers’ performance in hospitals.

     

    Methods: This correlational and cross-sectional research, was carried out on all the employees under the control of managers and chiefs of Qom province hospitals (N=2167. Sampling was done by cluster sampling method through the use of Cokran sampling based on Morgan and kerjsi chart. 998 samples with the confidence level of 95% and permitted errors of 0.05 were randomly selected. Data were collected via questionnaires which were answered by self-report method. The data were then analyzed by the Pearson correlation coefficient, variance's test F analyses, multiple variant regression and T-test.

     

    Results: The mean score of the manager's non-delegation of authority was (32.4. Among the indicators of the direction, there was a significant relationship between the motivation (mean= 61.44 and organizational communication (49.39, also there was a significant relationship between the managers’ delegation of authority and the manner of their employees direction (non-delegation of authority R=-0.13.

     

    Conclusion: Due to the meaningful and direct relationship between most of direction variables with the managers’ delegation of authority, increasing the delegation of authority and deconcentration can lead to an increase

  19. AGENCY PROBLEMS IN PUBLIC SECTOR

    Directory of Open Access Journals (Sweden)

    Gyorgy Attila

    2012-07-01

    Full Text Available Agency theory analyses the effects of contractual behaviour between two parties: principal(s and agent(s. This relation is inevitably characterized by information asymmetry because agent holds a substantially larger volume of information than the principal. Due the negative effects of information asymmetry for the principal, this should cover supplementary costs with monitoring agents and/or grant incentives. The first objective of this paper is to emphasize the effects of information asymmetry, particularly on adverse selection and moral hazard. The second objective is to evaluate the negative effects of information asymmetry and to assess the viability of solutions proposed by scholars for mitigation. The third objective is linked with personal contribution, respectively to highlight specificity of agency theory in public sector and the mechanisms of action in this particular field. In this paper, literature is mainly based on scholars’ contribution to the proposed theme. Little literature approaches agency theory in public sector, in most cases the analysis being restricted to general issues. Research methodology is based on synthesizing relevant information from literature and adapting them to public sector particularities. The results reflect some threats for public bodies in their contracting activity. Conclusions present also a set of solutions which could be used by public institutions to optimize their activity of mitigating information asymmetry’s effects. These solution guidelines could represent a useful instrument for make more efficient public money spending. Personal contribution and the novelty of this paper consist in presenting a new approach regarding mechanisms of managing information by agents. In case of public institutions, principals have more opportunities the take possession over the information managed by the agent. Nevertheless, agents can limit the principal’s access

  20. PICK1 regulates the trafficking of ASIC1a and acidotoxicity in a BAR domain lipid binding-dependent manner

    Directory of Open Access Journals (Sweden)

    Jin Wenying

    2010-12-01

    Full Text Available Abstract Background Acid-sensing ion channel 1a (ASIC1a is the major ASIC subunit determining acid-activated currents in brain neurons. Recent studies show that ASIC1a play critical roles in acid-induced cell toxicity. While these studies raise the importance of ASIC1a in diseases, mechanisms for ASIC1a trafficking are not well understood. Interestingly, ASIC1a interacts with PICK1 (protein interacting with C-kinase 1, an intracellular protein that regulates trafficking of several membrane proteins. However, whether PICK1 regulates ASIC1a surface expression remains unknown. Results Here, we show that PICK1 overexpression increases ASIC1a surface level. A BAR domain mutant of PICK1, which impairs its lipid binding capability, blocks this increase. Lipid binding of PICK1 is also required for PICK1-induced clustering of ASIC1a. Consistent with the effect on ASIC1a surface levels, PICK1 increases ASIC1a-mediated acidotoxicity and this effect requires both the PDZ and BAR domains of PICK1. Conclusions Taken together, our results indicate that PICK1 regulates trafficking and function of ASIC1a in a lipid binding-dependent manner.

  1. Both the cutaneous sensation and phosphene perception are modulated in a frequency-specific manner during transcranial alternating current stimulation.

    Science.gov (United States)

    Turi, Zs; Ambrus, G G; Janacsek, K; Emmert, K; Hahn, L; Paulus, W; Antal, A

    2013-01-01

    Transcranial alternating current stimulation (tACS) is a non-invasive stimulation technique for shaping neuroplastic processes and possibly entraining ongoing neural oscillations in humans. Despite the growing number of studies using tACS, we know little about the procedural sensations caused by stimulation. In order to fill this gap, we explored the cutaneous sensation and phosphene perception during tACS. Twenty healthy participants took part in a randomized, single-blinded, sham-controlled study, where volunteers received short duration stimulation at 1.0 mA intensity between 2 to 250 Hz using the standard left motor cortex-contralateral supraorbital montage. We recorded the perception onset latency and the strength of the sensations assessed by visual rating scale as dependent variables. We found that tACS evoked both cutaneous sensation and phosphene perception in a frequency-dependent manner. Our results show that the most perceptible procedural sensations were induced in the beta and gamma frequency range, especially at 20 Hz, whereas minimal procedural sensations were indicated in the ripple range (140 and 250 Hz). We believe that our results provide a relevant insight into the procedural sensations caused by oscillatory currents, and will offer a basis for developing more sophisticated stimulation protocols and study designs for future investigations.

  2. Fluoxetine regulates mTOR signalling in a region-dependent manner in depression-like mice

    Science.gov (United States)

    Liu, Xiao-Long; Luo, Liu; Mu, Rong-Hao; Liu, Bin-Bin; Geng, Di; Liu, Qing; Yi, Li-Tao

    2015-01-01

    Previous studies have demonstrated that the mammalian target of rapamycin (mTOR) signaling pathway has an important role in ketamine-induced, rapid antidepressant effects despite the acute administration of fluoxetine not affecting mTOR phosphorylation in the brain. However, the effects of long-term fluoxetine treatment on mTOR modulation have not been assessed to date. In the present study, we examined whether fluoxetine, a type of commonly used antidepressant agent, alters mTOR signaling following chronic administration in different brain regions, including the frontal cortex, hippocampus, amygdala and hypothalamus. We also investigated whether fluoxetine enhanced synaptic protein levels in these regions via the activation of the mTOR signaling pathway and its downstream regulators, p70S6K and 4E-BP-1. The results indicated that chronic fluoxetine treatment attenuated the chronic, unpredictable, mild stress (CUMS)-induced mTOR phosphorylation reduction in the hippocampus and amygdala of mice but not in the frontal cortex or the hypothalamus. Moreover, the CUMS-decreased PSD-95 and synapsin I levels were reversed by fluoxetine, and these effects were blocked by rapamycin only in the hippocampus. In conclusion, our findings suggest that chronic treatment with fluoxetine can induce synaptic protein expression by activating the mTOR signaling pathway in a region-dependent manner and mainly in the hippocampus. PMID:26522512

  3. Cholecalciferol attenuates perseverative behavior associated with developmental alcohol exposure in rats in a dose-dependent manner.

    Science.gov (United States)

    Idrus, N M; Happer, J P; Thomas, J D

    2013-07-01

    Alcohol is a known teratogen that is estimated to affect 2-5% of the births in the U.S. Prenatal alcohol exposure can produce physical features such as facial dysmorphology, physiological alterations such as cell loss in the central nervous system (CNS), and behavioral changes that include hyperactivity, cognitive deficits, and motor dysfunction. The range of effects associated with prenatal alcohol exposure is referred to as fetal alcohol spectrum disorders (FASD). Despite preventative measures, some women continue to drink while pregnant. Therefore, identifying interventions that reduce the severity of FASD is critical. This study investigated one such potential intervention, vitamin D3, a nutrient that exerts neuroprotective properties. The present study determined whether cholecalciferol, a common vitamin D3 nutritional supplement, could serve as a means of mitigating alcohol-related learning deficits. Using a rat model of FASD, cholecalciferol was given before, during, and after 3rd trimester equivalent alcohol exposure. Three weeks after cholecalciferol treatment, subjects were tested on a serial spatial discrimination reversal learning task. Animals exposed to ethanol committed significantly more errors compared to controls. Cholecalciferol treatment reduced perseverative behavior that is associated with developmental alcohol exposure in a dose-dependent manner. These data have important implications for the treatment of FASD and suggest that cholecalciferol may reduce some aspects of FASD. This article is part of a Special Issue entitled 'Vitamin D Workshop'. Copyright © 2012 Elsevier Ltd. All rights reserved.

  4. Category-selective attention interacts with partial awareness processes in a continuous manner: An fMRI study

    Directory of Open Access Journals (Sweden)

    Shen Tu

    2015-12-01

    Full Text Available Recently, our team found that category-selective attention could modulate tool processing at the partial awareness level and unconscious face processing in the middle occipital gyrus (MOG. However, the modulation effects in MOG were in opposite directions across the masked tool and masked face conditions in that study: MOG activation decreased in the masked faces condition but increased in the masked tools condition under the consistent compared with the inconsistent cue-selective-attentional modulation. In the present study, in order to confirm that the opposite effects were due to the changed contours of the tools, using the same tool pictures and fMRI technique, we devised another two conditions: variant mirror tool picture condition and invariant tool picture condition. The results showed that, during the variant mirror tool picture condition, activation in the MOG decreased under tool-selective attention compared with face-selective attention. Interestingly, however, during the invariant tool picture condition, activation in the MOG revealed neither positive nor negative changes. Combined with the result of increased MOG activity in the changed different tool condition, the three different effects demonstrated not only that the unconscious component of partial awareness processing (no knowledge of the identity of the tool could be modulated by the category-selective attention in the earlier visual cortex but also that the modulation effect could further interact with the conscious component of partial awareness processing (consciousness of the changing contour of the tool in a continuous manner.

  5. Light at night acutely impairs glucose tolerance in a time-, intensity- and wavelength-dependent manner in rats.

    Science.gov (United States)

    Opperhuizen, Anne-Loes; Stenvers, Dirk J; Jansen, Remi D; Foppen, Ewout; Fliers, Eric; Kalsbeek, Andries

    2017-07-01

    Exposure to light at night (LAN) has increased dramatically in recent decades. Animal studies have shown that chronic dim LAN induced obesity and glucose intolerance. Furthermore, several studies in humans have demonstrated that chronic exposure to artificial LAN may have adverse health effects with an increased risk of metabolic disorders, including type 2 diabetes. It is well-known that acute exposure to LAN affects biological clock function, hormone secretion and the activity of the autonomic nervous system, but data on the effects of LAN on glucose homeostasis are lacking. This study aimed to investigate the acute effects of LAN on glucose metabolism. Male Wistar rats were subjected to i.v. glucose or insulin tolerance tests while exposed to 2 h of LAN in the early or late dark phase. In subsequent experiments, different light intensities and wavelengths were used. LAN exposure early in the dark phase at ZT15 caused increased glucose responses during the first 20 min after glucose infusion (p light of 50 and 150 lx induced greater glucose responses than 5 and 20 lx, whereas all intensities other than 5 lx reduced locomotor activity. Green light induced glucose intolerance, but red and blue light did not, suggesting the involvement of a specific retina-brain pathway. Together, these data show that exposure to LAN has acute adverse effects on glucose metabolism in a time-, intensity- and wavelength-dependent manner.

  6. Indoor visible mold and mold odor are associated with new-onset childhood wheeze in a dose-dependent manner.

    Science.gov (United States)

    Shorter, Caroline; Crane, Julian; Pierse, Nevil; Barnes, Phillipa; Kang, Janice; Wickens, Kristin; Douwes, Jeroen; Stanley, Thorsten; Täubel, Martin; Hyvärinen, Anne; Howden-Chapman, Philippa

    2018-01-01

    Evidence is accumulating that indoor dampness and mold are associated with the development of asthma. The underlying mechanisms remain unknown. New Zealand has high rates of both asthma and indoor mold and is ideally placed to investigate this. We conducted an incident case-control study involving 150 children with new-onset wheeze, aged between 1 and 7 years, each matched to two control children with no history of wheezing. Each participant's home was assessed for moisture damage, condensation, and mold growth by researchers, an independent building assessor and parents. Repeated measures of temperature and humidity were made, and electrostatic dust cloths were used to collect airborne microbes. Cloths were analyzed using qPCR. Children were skin prick tested for aeroallergens to establish atopy. Strong positive associations were found between observations of visible mold and new-onset wheezing in children (adjusted odds ratios ranged between 1.30 and 3.56; P ≤ .05). Visible mold and mold odor were consistently associated with new-onset wheezing in a dose-dependent manner. Measurements of qPCR microbial levels, temperature, and humidity were not associated with new-onset wheezing. The association between mold and new-onset wheeze was not modified by atopic status, suggesting a non-allergic association. © 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  7. Transcriptional repressor domain of MBD1 is intrinsically disordered and interacts with its binding partners in a selective manner.

    KAUST Repository

    Hameed, Umar Farook Shahul

    2014-05-09

    Methylation of DNA CpG sites is a major mechanism of epigenetic gene silencing and plays important roles in cell division, development and carcinogenesis. One of its regulators is the 64-residue C-terminal Transcriptional Repressor Domain (the TRD) of MBD1, which recruits several repressor proteins such as MCAF1, HDAC3 and MPG that are essential for the gene silencing. Using NMR spectroscopy, we have characterized the solution structure of the C-terminus of MBD1 (MBD1-c, residues D507 to Q605), which included the TRD (A529 to P592). Surprisingly, the MBD1-c is intrinsically disordered. Despite its lack of a tertiary folding, MBD1-c could still bind to different partner proteins in a selective manner. MPG and MCAF1Δ8 showed binding to both the N-terminal and C-terminal residues of MBD1-c but HDAC3 preferably bound to the C-terminal region. This study reveals how MBD1-c discriminates different binding partners, and thus, expands our understanding of the mechanisms of gene regulation by MBD1.

  8. Ecklonia cava Polyphenol Has a Protective Effect against Ethanol-Induced Liver Injury in a Cyclic AMP-Dependent Manner

    Directory of Open Access Journals (Sweden)

    Haruka Yamashita

    2015-06-01

    Full Text Available Previously, we showed that Ecklonia cava polyphenol (ECP treatment suppressed ethanol-induced increases in hepatocyte death by scavenging intracellular reactive oxygen species (ROS and maintaining intracellular glutathione levels. Here, we examined the effects of ECP on the activities of alcohol-metabolizing enzymes and their regulating mechanisms in ethanol-treated hepatocytes. Isolated hepatocytes were incubated with or without 100 mM ethanol. ECP was dissolved in dimethylsulfoxide. ECP was added to cultured cells that had been incubated with or without ethanol. The cells were incubated for 0–24 h. In cultured hepatocytes, the ECP treatment with ethanol inhibited cytochrome P450 2E1 (CYP2E1 expression and activity, which is related to the production of ROS when large quantities of ethanol are oxidized. On the other hand, ECP treatment with ethanol increased the activity of alcohol dehydrogenase (ADH and aldehyde dehydrogenase. These changes in activities of CYP2E1 and ADH were suppressed by treatment with H89, an inhibitor of protein kinase A. ECP treatment with ethanol enhanced cyclic AMP concentrations compared with those of control cells. ECP may be a candidate for preventing ethanol-induced liver injury via regulating alcohol metabolic enzymes in a cyclic AMP-dependent manner.

  9. Transgenic over-expression of YY1 induces pathologic cardiac hypertrophy in a sex-specific manner

    Science.gov (United States)

    Stauffer, Brian L.; Dockstader, Karen; Russell, Gloria; Hijmans, Jamie; Walker, Lisa; Cecil, Mackenzie; Demos-Davies, Kimberly; Medway, Allen; McKinsey, Timothy A.; Sucharov, Carmen C.

    2015-01-01

    YY1 can activate or repress transcription of various genes. In cardiac myocytes in culture YY1 has been shown to regulate expression of several genes involved in myocyte pathology. YY1 can also acutely protect the heart against detrimental changes in gene expression. In this study we show that cardiac over-expression of YY1 induces pathologic cardiac hypertrophy in male mice, measured by changes in gene expression and lower ejection fraction/fractional shortening. In contrast, female animals are protected against pathologic gene expression changes and cardiac dysfunction. Furthermore, we show that YY1 regulates, in a sex-specific manner, the expression of mammalian enable (Mena), a factor that regulates cytoskeletal actin dynamics and whose expression is increased in several models of cardiac pathology, and that Mena expression in humans with heart failure is sex-dependent. Finally, we show that sex differences in YY1 expression are also observed in human heart failure. In summary, this is the first work to show that YY1 has a sex-specific effect in the regulation of cardiac pathology. PMID:25935483

  10. Common inversion polymorphism at 17q21.31 affects expression of multiple genes in tissue-specific manner.

    Science.gov (United States)

    de Jong, Simone; Chepelev, Iouri; Janson, Esther; Strengman, Eric; van den Berg, Leonard H; Veldink, Jan H; Ophoff, Roel A

    2012-09-06

    Chromosome 17q21.31 contains a common inversion polymorphism of approximately 900 kb in populations with European ancestry. Two divergent MAPT haplotypes, H1 and H2 are described with distinct linkage disequilibrium patterns across the region reflecting the inversion status at this locus. The MAPT H1 haplotype has been associated with progressive supranuclear palsy, corticobasal degeneration, Parkinson's disease and Alzheimer's disease, while the H2 is linked to recurrent deletion events associated with the 17q21.31 microdeletion syndrome, a disease characterized by developmental delay and learning disability. In this study, we investigate the effect of the inversion on the expression of genes in the 17q21.31 region. We find the expression of several genes in and at the borders of the inversion to be affected; specific either to whole blood or different regions of the human brain. The H1 haplotype was found to be associated with an increased expression of LRRC37A4, PLEKH1M and MAPT. In contrast, a decreased expression of MGC57346, LRRC37A and CRHR1 was associated with H1. Studies thus far have focused on the expression of MAPT in the inversion region. However, our results show that the inversion status affects expression of other genes in the 17q21.31 region as well. Given the link between the inversion status and different neurological diseases, these genes may also be involved in disease pathology, possibly in a tissue-specific manner.

  11. Rhizoctonia solani and Bacterial Inoculants Stimulate Root Exudation of Antifungal Compounds in Lettuce in a Soil-Type Specific Manner

    Directory of Open Access Journals (Sweden)

    Saskia Windisch

    2017-06-01

    Full Text Available Previous studies conducted on a unique field site comprising three contrasting soils (diluvial sand DS, alluvial loam AL, loess loam LL under identical cropping history, demonstrated soil type-dependent differences in biocontrol efficiency against Rhizoctonia solani-induced bottom rot disease in lettuce by two bacterial inoculants (Pseudomonas jessenii RU47 and Serratia plymuthica 3Re-4-18. Disease severity declined in the order DS > AL > LL. These differences were confirmed under controlled conditions, using the same soils in minirhizotron experiments. Gas chromatography-mass spectrometry (GC-MS profiling of rhizosphere soil solutions revealed benzoic and lauric acids as antifungal compounds; previously identified in root exudates of lettuce. Pathogen inoculation and pre-inoculation with bacterial inoculants significantly increased the release of antifungal root exudates in a soil type-specific manner; with the highest absolute levels detected on the least-affected LL soil. Soil type-dependent differences were also recorded for the biocontrol effects of the two bacterial inoculants; showing the highest efficiency after double-inoculation on the AL soil. However, this was associated with a reduction of shoot growth and root hair development and a limited micronutrient status of the host plants. Obviously, disease severity and the expression of biocontrol effects are influenced by soil properties with potential impact on reproducibility of practical applications.

  12. He-Ne laser irradiation affects proliferation of cultured rat Schwann cells in a dose-dependent manner

    International Nuclear Information System (INIS)

    Breugel, H.H.F.I. van; Bar, P.R.

    1993-01-01

    Schwann cell proliferation is considered an essential part of Wallerian degeneration after nerve damage. Laminin, an important component of the extracellular matrix and produced by Schwann cells, provides a preferred substrate for outgrowing axons. To study whether low energy (He-Ne) laser irradiation may exert a positive effect on nerve regeneration through an effect on Schwann cells, its effect was evaluated in vitro. Schwann cells were isolated from sciatic nerves of 4-5-day old Wistar rats and cultures on 96-multiwell plates. The cells were irradiated by a He-Ne laser beam. At three consecutive days, starting either at day 5 or day 8, cells were irradiated each day for 0.5, 1, 2, 5 or 10 min. Both cell number and laminin production were determined for each irradiation condition within one experiment. Schwann cells that were irradiated from day 8 on were hardly affected by laser irradiation. However, the proliferation of cells that were irradiated starting on day 5 was significantly increased after 1, 2 and 5 min of daily irradiation, compared to non-irradiated control cultures. The lamin production per cell of these Schwann cells was not significantly altered. From these results we conclude that He-Ne laser irradiation can modulate proliferation of rat Schwann cells in vitro in a dose-dependent manner. (Author)

  13. Serotonin inputs to the dorsal BNST modulate anxiety in a 5-HT1A receptor dependent manner

    Science.gov (United States)

    Garcia-Garcia, Alvaro L.; Canetta, Sarah; Stujenske, Joseph M.; Burghardt, Nesha S.; Ansorge, Mark S.; Dranovsky, Alex; Leonardo, E. David

    2017-01-01

    Serotonin (5-HT) neurons project from the raphe nuclei throughout the brain where they act to maintain homeostasis. Here, we study 5-HT inputs into the bed nucleus of the stria terminalis (BNST), a major subdivision of the extended amygdala that has been proposed to regulate responses to anxiogenic environments in humans and rodents. While the dorsal part of the BNST (dBNST) receives dense 5-HT innervation, whether and how 5-HT in the dBNST normally modulates anxiety remains unclear. Using optogenetics, we demonstrate that activation of 5-HT terminals in the dBNST reduces anxiety in a highly anxiogenic environment. Further analysis revealed that optogenetic inhibition of 5-HT inputs into the dBNST increases anxiety in a less anxiogenic environment. We found that 5-HT predominantly hyperpolarizes dBNST neurons, reducing their activity in a manner that can be blocked by a 5-HT1A antagonist. Finally, we demonstrate that activation of 5-HT1A receptors in the dBNST is necessary for the anxiolytic effect observed following optogenetic stimulation of 5-HT inputs into the dBNST. These data reveal that 5-HT release in the dBNST modulates anxiety-like behavior via 5-HT1A receptors under naturalistic conditions. PMID:28761080

  14. Pharmacological inhibition of lysosomes activates the MTORC1 signaling pathway in chondrocytes in an autophagy-independent manner.

    Science.gov (United States)

    Newton, Phillip T; Vuppalapati, Karuna K; Bouderlique, Thibault; Chagin, Andrei S

    2015-01-01

    Mechanistic target of rapamycin (serine/threonine kinase) complex 1 (MTORC1) is a protein-signaling complex at the fulcrum of anabolic and catabolic processes, which acts depending on wide-ranging environmental cues. It is generally accepted that lysosomes facilitate MTORC1 activation by generating an internal pool of amino acids. Amino acids activate MTORC1 by stimulating its translocation to the lysosomal membrane where it forms a super-complex involving the lysosomal-membrane-bound vacuolar-type H(+)-ATPase (v-ATPase) proton pump. This translocation and MTORC1 activation require functional lysosomes. Here we found that, in contrast to this well-accepted concept, in epiphyseal chondrocytes inhibition of lysosomal activity by v-ATPase inhibitors bafilomycin A1 or concanamycin A potently activated MTORC1 signaling. The activity of MTORC1 was visualized by phosphorylated forms of RPS6 (ribosomal protein S6) and EIF4EBP1, 2 well-known downstream targets of MTORC1. Maximal RPS6 phosphorylation was observed at 48-h treatment and reached as high as a 12-fold increase (p lysosomes. Thus, our data show that in epiphyseal chondrocytes lysosomes inhibit MTORC1 in a macroautophagy-independent manner and this inhibition likely depends on v-ATPase activity.

  15. Maternal exposure to environmental enrichment before and during gestation influences behaviour of rat offspring in a sex-specific manner.

    Science.gov (United States)

    Zuena, Anna Rita; Zinni, Manuela; Giuli, Chiara; Cinque, Carlo; Alemà, Giovanni Sebastiano; Giuliani, Alessandro; Catalani, Assia; Casolini, Paola; Cozzolino, Roberto

    2016-09-01

    The beneficial effects of Environmental Enrichment (EE) applied immediately after weaning or even in adulthood have been widely demonstrated. Less is known about the possible changes in behaviour and brain development of the progeny following the exposure of dams to EE. In order to further investigate this matter, female rats were reared in EE for 12weeks, from weaning until delivery. After having confirmed the presence of relevant behavioural effects of EE, both control and EE females underwent mating. Maternal behaviour was observed and male and female offspring were then administered a battery of behavioural test at different ages. EE mothers showed a decreased frequency of total nursing and, during the first 2days of lactation, an increase in licking/grooming behaviour. Maternal exposure to EE affected offspring behaviour in a sex-specific manner: social play behaviour and anxiety-like behaviour were increased in males but not in females and learning ability was improved only in females. As a general trend, maternal EE had a marked influence on motility in male and female offspring in both locomotor activity and swimming speed. Overall, this study highlights the importance of environmental stimulation, not only in the animals directly experiencing EE, but for their progeny too, opening the way to new hypothesis on the heritability mechanisms of behavioural traits. Copyright © 2016 Elsevier Inc. All rights reserved.

  16. Transcriptional repressor domain of MBD1 is intrinsically disordered and interacts with its binding partners in a selective manner.

    KAUST Repository

    Hameed, Umar Farook Shahul; Lim, Jackwee; Zhang, Qian; Wasik, Mariusz A; Yang, Daiwen; Swaminathan, Kunchithapadam

    2014-01-01

    Methylation of DNA CpG sites is a major mechanism of epigenetic gene silencing and plays important roles in cell division, development and carcinogenesis. One of its regulators is the 64-residue C-terminal Transcriptional Repressor Domain (the TRD) of MBD1, which recruits several repressor proteins such as MCAF1, HDAC3 and MPG that are essential for the gene silencing. Using NMR spectroscopy, we have characterized the solution structure of the C-terminus of MBD1 (MBD1-c, residues D507 to Q605), which included the TRD (A529 to P592). Surprisingly, the MBD1-c is intrinsically disordered. Despite its lack of a tertiary folding, MBD1-c could still bind to different partner proteins in a selective manner. MPG and MCAF1Δ8 showed binding to both the N-terminal and C-terminal residues of MBD1-c but HDAC3 preferably bound to the C-terminal region. This study reveals how MBD1-c discriminates different binding partners, and thus, expands our understanding of the mechanisms of gene regulation by MBD1.

  17. Peripubertal Caffeine Exposure Impairs Longitudinal Bone Growth in Immature Male Rats in a Dose- and Time-Dependent Manner.

    Science.gov (United States)

    Choi, Yun-Young; Choi, Yuri; Kim, Jisook; Choi, Hyeonhae; Shin, Jiwon; Roh, Jaesook

    2016-01-01

    This study investigated the dose- and time-dependent effects of caffeine consumption throughout puberty in peripubertal rats. A total of 85 male SD rats were randomly divided into four groups: control and caffeine-fed groups with 20, 60, or 120 mg/kg/day through oral gavage for 10, 20, 30, or 40 days. Caffeine decreased body weight gain and food consumption in a dose- and time-dependent manner, accompanied by a reduction in muscle and body fat. In addition, it caused a shortening and lightening of leg bones and spinal column. The total height of the growth plate decreased sharply at 40 days in the controls, but not in the caffeine-fed groups, and the height of hypertrophic zone in the caffeine-fed groups was lower than in the control. Caffeine increased the height of the secondary spongiosa, whereas parameters related to bone formation, such as bone area ratio, thickness and number of trabeculae, and bone perimeter, were significantly reduced. Furthermore, serum levels of IGF-1, estradiol, and testosterone were also reduced by the dose of caffeine exposure. Our results demonstrate that caffeine consumption can dose- and time-dependently inhibit longitudinal bone growth in immature male rats, possibly by blocking the physiologic changes in body composition and hormones relevant to bone growth.

  18. Public values in public-private partnerships

    NARCIS (Netherlands)

    Reynaers, A.

    2014-01-01

    Although public-private partnerships (PPPs) are frequently analyzed and lauded in terms of efficiency, their impact on public values is often neglected. As a result, there is little empirical evidence supporting or rejecting the claim that PPPs have a negative effect on public values. This case

  19. Nuclear law and public acceptance

    International Nuclear Information System (INIS)

    Muguet, Tania Mara F.

    2009-01-01

    After the fission of the atom and its use for military purposes, the imposition of controls and restrictions to prevent the proliferation of atomic weapons was established and led to the drafting of a series of international conventions to promote the harmonization of domestic legislation. In this context, to provide a legal framework for conducting activities related to nuclear energy and ionizing radiation, in a manner that adequately protects individuals, property and environment, the nuclear energy law was created and widely adopted. To better control the risks associated with the use of nuclear energy and in order to adapt its technological developments in constant state of evolution, a growing body of international law of nuclear energy is emerging from instruments (universal, regional, bilateral and multilateral) to impose obligation in the use of the technology. In sum, changes in technological, economic, political or social conditions created the need for legal solutions and the public understanding and confidence in the peaceful uses of nuclear energy requires extensive information to be made available on the risks and benefits to stakeholders (effected public, press, media, and legislators etc.). (author)

  20. Nuclear law and public acceptance

    Energy Technology Data Exchange (ETDEWEB)

    Muguet, Tania Mara F. [Comissao Nacional de Energia Nuclear (CNEN), Rio de Janeiro, RJ (Brazil). Coordenacao Geral de Assuntos Internacionais], e-mail: tmuguet@cnen.gov.br

    2009-07-01

    After the fission of the atom and its use for military purposes, the imposition of controls and restrictions to prevent the proliferation of atomic weapons was established and led to the drafting of a series of international conventions to promote the harmonization of domestic legislation. In this context, to provide a legal framework for conducting activities related to nuclear energy and ionizing radiation, in a manner that adequately protects individuals, property and environment, the nuclear energy law was created and widely adopted. To better control the risks associated with the use of nuclear energy and in order to adapt its technological developments in constant state of evolution, a growing body of international law of nuclear energy is emerging from instruments (universal, regional, bilateral and multilateral) to impose obligation in the use of the technology. In sum, changes in technological, economic, political or social conditions created the need for legal solutions and the public understanding and confidence in the peaceful uses of nuclear energy requires extensive information to be made available on the risks and benefits to stakeholders (effected public, press, media, and legislators etc.). (author)

  1. How to gather information from talkative patients in a respectful and efficient manner: a qualitative study of GPs' communication strategies.

    Science.gov (United States)

    Giroldi, Esther; Veldhuijzen, Wemke; Dijkman, Annika; Rozestraten, Maxime; Muris, Jean; van der Vleuten, Cees; van der Weijden, Trudy

    2016-02-01

    Gathering information from talkative patients presents a challenge to clinicians. Empirical evidence on how to effectively deal with this challenge is scant. This study explores communication strategies and their underlying mechanisms that GPs consider effective when gathering information from talkative patients in order to inform the development of best practices. We conducted a qualitative study with experienced GPs. We held individual stimulated-recall interviews (SRIs) with six GPs using their videotaped consultations as a stimulus. The transcripts that ensued were triangulated with data from three focus-group discussions (FGs). We performed a thematic network analysis during an iterative process of data collection and analysis. To deal with talkative patients during consultations, GPs first try to pinpoint the cause of patients' talkativeness before deciding on the approach to take. Moreover, they resort to the familiar communication strategies, however, in doing so adopt take a more directive attitude. To prevent such attitude from damaging the relationship, GPs take a stepped approach in which they try not to be overly directive, make the patient co-responsible for efficient time management and make use of empathic interrupting. In the absence of evidence, this description of GPs' communication strategies can guide clinicians, residents and students in gathering information from talkative patients in an efficient, yet empathic and respectful manner. When developing best practices, heed should be paid to the causes of patients' talkativeness and the tension between taking a directive approach and building a doctor-patient relationship. © The Author 2015. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  2. TLR3 mediates release of IL-1β and cell death in keratinocytes in a caspase-4 dependent manner.

    Science.gov (United States)

    Grimstad, Øystein; Husebye, Harald; Espevik, Terje

    2013-10-01

    Inflammation and timely cell death are important elements in host defence and healing processes. Keratinocytes express high levels of Toll-like receptor 3 (TLR3), and stimulation of the receptor with its ligand polyinosinic-polycytidylic acid (polyI:C) is a powerful signal for release of a variety of proinflammatory cytokines. Caspase-4 is required for maturation of pro-IL-1β through activation of caspase-1 in keratinocytes. TLR3 in keratinocytes was stimulated with polyI:C. Induction of messenger RNA of pro-IL-1β and inflammasomal components was measured using quantitative polymerase chain reaction methodology. Protein expression of IL-1β was analysed with ELISA and Western blot techniques. Activation of apoptotic caspases was measured with flow cytometry, and cytotoxicity was determined. TLR3 induced release of substantial amounts of pro-IL-1β in keratinocytes. NLRP3 or ASC dependent processing of IL-1β into its cleaved bioactive form was found to be minimal. The release of IL-1β was due to polyI:C induced cell death that occurred through a caspase-4 dependent manner. Caspase-1 did not seem to be involved in the polyI:C induced cytotoxicity despite that TLR3 stimulation induced activation of caspase-1. In addition, the apoptotic caspases -8, -9 and -3/7 were activated by polyI:C. TLR3 stimulation in keratinocytes induces a caspase-4 dependent release of pro-IL-1β, but further processing to active IL-1β is limited. Furthermore, TLR3 stimulation results in pyroptotic- and apoptotic cell death. Copyright © 2013 Japanese Society for Investigative Dermatology. Published by Elsevier Ireland Ltd. All rights reserved.

  3. Moringa oleifera's Nutritious Aqueous Leaf Extract Has Anticancerous Effects by Compromising Mitochondrial Viability in an ROS-Dependent Manner.

    Science.gov (United States)

    Madi, Niveen; Dany, Mohammed; Abdoun, Salah; Usta, Julnar

    2016-01-01

    Moringa oleifera (MO) is an important dietary component for many populations in West Africa and the Indian subcontinent. In addition to its highly nutritious value, almost all parts of this plant have been widely used in folk medicine in curing infectious, cardiovascular, gastrointestinal, hepatic, and other diseases. Evidence-based research supported its versatile medicinal properties; however, more rigorous research is required to establish it in cancer therapy. As such, in this study we aim to investigate the in vitro anticancerous effect of Moringa oleifera's aqueous leaf extract. Moringa extract was prepared by soaking pulverized leaves in hot water mimicking the people's mode of the leaf drink preparation. Several assays were used to study the effect of different percentage concentrations of the extract on viability of A549 cells; levels of adenosine triphosphate (ATP), reactive oxygen species (ROS), and glutathione (GSH) generated; as well as percentage of lactate dehydrogenase (LDH) released at different time points. In addition to mitochondrial membrane potential, apoptotic events were assessed using western blotting for apoptotic markers and immunoflourescent flourescent labeled inhibitor of caspases (FLICA) assay. MO extract treatment resulted in a significant decrease in mitochondrial membrane potential (1 hour) and ATP levels (3 hours), followed by an increase in (6 hours) ROS, caspase activation, proapoptotic proteins expression (p53, SMAC/Diablo, AIF), and PARP-1 cleavage. This eventually resulted in decreased GSH levels and a decrease in viability. The cytotoxic effect was prevented upon pretreatment with antioxidant N-acetyl-cysteine. MO decreased as well the viability of HepG2, CaCo2, Jurkat, and HEK293 cells. Our findings identify a plant extract with an anticancerous effect on cancer cell lines. MO extract exerts its cytotoxic effect in A549 cancer cells by affecting mitochondrial viability and inducing apoptosis in an ROS-dependent manner.

  4. Respiratory function in healthy ever-smokers is impaired by smoking habits in a dose-dependent manner.

    Science.gov (United States)

    Osanai, Shinobu; Ogasa, Toshiyuki; Sumitomo, Kazuhiro; Hasebe, Naoyuki

    2018-01-01

    There is limited information about the respiratory function of ever-smokers without lung disorders. We sought to assess the effects of smoking habits on respiratory function in subjects without lung disorders. Subjects were recruited from among patients without any evidence of respiratory disorders who visited rural primary care clinics. Each participant was asked to answer a questionnaire that included questions smoking history. Their forced vital capacity (FVC) and forced expiratory volume in one second (FEV1) were measured. We analyzed 802 subjects (364 men and 438 women). The means of the lambda-mu-sigma method derived z-score of FEV1 (zFEV1) both in current-smokers and ex-smokers were lower than that in never-smokers. The mean zFEV1 in the ever-smokers with more than 30 pack-years of smoking history were lower than that in the ever-smokers with less smoking history. Univariate analysis showed that there were significant negative correlations between pack-years and zFEV1 both in the ex-smokers and current-smokers. There was no significant correlation between the duration of smoking cessation and zFEV1 in the ex-smokers. Our data suggests that respiratory function in healthy ever-smokers is decreased based on smoking habits in a dose-dependent manner. Even after a long period of smoking cessation, the decreased respiratory function seems to be maintained in ex-smokers. Copyright © 2017 The Japanese Respiratory Society. Published by Elsevier B.V. All rights reserved.

  5. Insulin is essential for in vitro chondrogenesis of mesenchymal progenitor cells and influences chondrogenesis in a dose-dependent manner.

    Science.gov (United States)

    Mueller, Michael B; Blunk, Torsten; Appel, Bernhard; Maschke, Angelika; Goepferich, Achim; Zellner, Johannes; Englert, Carsten; Prantl, Lukas; Kujat, Richard; Nerlich, Michael; Angele, Peter

    2013-01-01

    Insulin is a commonly used additive in chondrogenic media for differentiating mesenchymal stem cells (MSCs). The indispensability of other bioactive factors like TGF-β or dexamethasone in these medium formulations has been shown, but the role of insulin is unclear. The purpose of this study was to investigate whether insulin is essential for MSC chondrogenesis and if there is a dose-dependent effect of insulin on MSC chondrogenesis. We cultivated human MSCs in pellet culture in serum-free chondrogenic medium with insulin concentrations between 0 and 50 μg/ml and assessed the grade of chondrogenic differentiation by histological evaluation and determination of glycosaminoglycan (GAG), total collagen and DNA content. We further tested whether insulin can be delivered in an amount sufficient for MSC chondrogenesis via a drug delivery system in insulin-free medium. Chondrogenesis was not induced by standard chondrogenic medium without insulin and the expression of cartilage differentiation markers was dose-dependent at insulin concentrations between 0 and 10 μg/ml. An insulin concentration of 50 μg/ml had no additional effect compared with 10 μg/ml. Insulin was delivered by a release system into the cell culture under insulin-free conditions in an amount sufficient to induce chondrogenesis. Insulin is essential for MSC chondrogenesis in this system and chondrogenic differentiation is influenced by insulin in a dose-dependent manner. Insulin can be provided in a sufficient amount by a drug delivery system. Therefore, insulin is a suitable and inexpensive indicator substance for testing drug release systems in vitro.

  6. An Aphid Effector Targets Trafficking Protein VPS52 in a Host-Specific Manner to Promote Virulence1[OPEN

    Science.gov (United States)

    2017-01-01

    Plant- and animal-feeding insects secrete saliva inside their hosts, containing effectors, which may promote nutrient release and suppress immunity. Although for plant pathogenic microbes it is well established that effectors target host proteins to modulate host cell processes and promote disease, the host cell targets of herbivorous insects remain elusive. Here, we show that the existing plant pathogenic microbe effector paradigm can be extended to herbivorous insects in that effector-target interactions inside host cells modify critical host processes to promote plant susceptibility. We showed that the effector Mp1 from Myzus persicae associates with the host Vacuolar Protein Sorting Associated Protein52 (VPS52). Using natural variants, we provide a strong link between effector virulence activity and association with VPS52, and show that the association is highly specific to M. persicae-host interactions. Also, coexpression of Mp1, but not Mp1-like variants, specifically with host VPS52s resulted in effector relocalization to vesicle-like structures that associate with prevacuolar compartments. We show that high VPS52 levels negatively impact virulence, and that aphids are able to reduce VPS52 levels during infestation, indicating that VPS52 is an important virulence target. Our work is an important step forward in understanding, at the molecular level, how a major agricultural pest promotes susceptibility during infestation of crop plants. We give evidence that an herbivorous insect employs effectors that interact with host proteins as part of an effective virulence strategy, and that these effectors likely function in a species-specific manner. PMID:28100451

  7. An Aphid Effector Targets Trafficking Protein VPS52 in a Host-Specific Manner to Promote Virulence.

    Science.gov (United States)

    Rodriguez, Patricia A; Escudero-Martinez, Carmen; Bos, Jorunn I B

    2017-03-01

    Plant- and animal-feeding insects secrete saliva inside their hosts, containing effectors, which may promote nutrient release and suppress immunity. Although for plant pathogenic microbes it is well established that effectors target host proteins to modulate host cell processes and promote disease, the host cell targets of herbivorous insects remain elusive. Here, we show that the existing plant pathogenic microbe effector paradigm can be extended to herbivorous insects in that effector-target interactions inside host cells modify critical host processes to promote plant susceptibility. We showed that the effector Mp1 from Myzus persicae associates with the host Vacuolar Protein Sorting Associated Protein52 (VPS52). Using natural variants, we provide a strong link between effector virulence activity and association with VPS52, and show that the association is highly specific to M persicae -host interactions. Also, coexpression of Mp1, but not Mp1-like variants, specifically with host VPS52s resulted in effector relocalization to vesicle-like structures that associate with prevacuolar compartments. We show that high VPS52 levels negatively impact virulence, and that aphids are able to reduce VPS52 levels during infestation, indicating that VPS52 is an important virulence target. Our work is an important step forward in understanding, at the molecular level, how a major agricultural pest promotes susceptibility during infestation of crop plants. We give evidence that an herbivorous insect employs effectors that interact with host proteins as part of an effective virulence strategy, and that these effectors likely function in a species-specific manner. © 2017 American Society of Plant Biologists. All Rights Reserved.

  8. Immobilization of immunoglobulin G in a highly oriented manner on a protein-A terminated multilayer system

    Energy Technology Data Exchange (ETDEWEB)

    Zengin, Adem [Department of Chemistry, Faculty of Art and Science, Gazi University, 06500 Besevler, Ankara (Turkey); Caykara, Tuncer, E-mail: caykara@gazi.edu.tr [Department of Chemistry, Faculty of Art and Science, Gazi University, 06500 Besevler, Ankara (Turkey)

    2011-01-01

    In this study, we have fabricated a multilayer system consisting of 3-glycidoxypropyldimethylmethoxysilane (GPDS), poly(dimethylsiloxane) bis 3-aminopropyl terminated (PDMS) and protein-A on a silicon wafer surface for oriented immobilization of immunoglobilin G (IgG). The multilayer system with a different component in each layer was characterized by ellipsometry, contact-angle goniometer, X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM) and fluorescence microscopy. The epoxy-terminated monolayer was formed by the chemisorption of GPDS molecules on the hydroxylated silicon surface. The PDMS film about 4.5 nm thick was produced on the GPDS-monolayer by the chemical reaction between the amine groups at the end of PDMS chain and the epoxy groups of GPDS molecules. By introducing the PDMS chains, the hydrophilic character of GPDS-monolayer decreased. Study of the time dependence of polymer grafting showed that the chemisorption of GPDS is fast, whereas at least 16 h is needed to generate the homogeneous PDMS layer. For immobilization of IgG molecules in a highly oriented manner, protein-A molecules were first chemically bound to an ultrathin ({approx}4.5 nm) PDMS reactive polymer layer and later used to capture IgG. It was shown that the existence of protein-A in the multilayer system has a strong influence on the binding properties of IgG not only in the efficiency of binding, but also in its specificity. In conclusion, the multilayer system with protein-A has the potential to be further developed into an efficient immunoassay protein chip.

  9. Parp1 protects against Aag-dependent alkylation-induced nephrotoxicity in a sex-dependent manner.

    Science.gov (United States)

    Calvo, Jennifer A; Allocca, Mariacarmela; Fake, Kimberly R; Muthupalani, Sureshkumar; Corrigan, Joshua J; Bronson, Roderick T; Samson, Leona D

    2016-07-19

    Nephrotoxicity is a common toxic side-effect of chemotherapeutic alkylating agents. Although the base excision repair (BER) pathway is essential in repairing DNA alkylation damage, under certain conditions the initiation of BER produces toxic repair intermediates that damage healthy tissues. We have shown that the alkyladenine DNA glycosylase, Aag (a.k.a. Mpg), an enzyme that initiates BER, mediates alkylation-induced whole-animal lethality and cytotoxicity in the pancreas, spleen, retina, and cerebellum, but not in the kidney. Cytotoxicity in both wild-type and Aag-transgenic mice (AagTg) was abrogated in the absence of Poly(ADP-ribose) polymerase-1 (Parp1). Here we report that Parp1-deficient mice expressing increased Aag (AagTg/Parp1-/-) develop sex-dependent kidney failure upon exposure to the alkylating agent, methyl methanesulfonate (MMS), and suffer increased whole-animal lethality compared to AagTg and wild-type mice. Macroscopic, histological, electron microscopic and immunohistochemical analyses revealed morphological kidney damage including dilated tubules, proteinaceous casts, vacuolation, collapse of the glomerular tuft, and deterioration of podocyte structure. Moreover, mice exhibited clinical signs of kidney disease indicating functional damage, including elevated blood nitrogen urea and creatinine, hypoproteinemia and proteinuria. Pharmacological Parp inhibition in AagTg mice also resulted in sensitivity to MMS-induced nephrotoxicity. These findings provide in vivo evidence that Parp1 modulates Aag-dependent MMS-induced nephrotoxicity in a sex-dependent manner and highlight the critical roles that Aag-initiated BER and Parp1 may play in determining the side-effects of chemotherapeutic alkylating agents.

  10. Maternal obesity alters brain derived neurotrophic factor (BDNF) signaling in the placenta in a sexually dimorphic manner.

    Science.gov (United States)

    Prince, Calais S; Maloyan, Alina; Myatt, Leslie

    2017-01-01

    Obesity is a major clinical problem in obstetrics being associated with adverse pregnancy outcomes and fetal programming. Brain derived neurotrophic factor (BDNF), a validated miR-210 target, is necessary for placental development, fetal growth, glucose metabolism, and energy homeostasis. Plasma BDNF levels are reduced in obese individuals; however, placental BDNF has yet to be studied in the context of maternal obesity. In this study, we investigated the effect of maternal obesity and sexual dimorphism on placental BDNF signaling. BDNF signaling was measured in placentas from lean (pre-pregnancy BMI 30) women at term without medical complications that delivered via cesarean section without labor. MiRNA-210, BDNF mRNA, proBDNF, and mature BDNF were measured by RT - PCR, ELISA, and Western blot. Downstream signaling via TRKB (BDNF receptor) was measured using Western blot. Maternal obesity was associated with increased miRNA-210 and decreased BDNF mRNA in placentas from female fetuses, and decreased proBDNF in placentas from male fetuses. We also identified decreased mature BDNF in placentas from male fetuses when compared to female fetuses. Mir-210 expression was negatively correlated with mature BDNF protein. TRKB phosphorylated at tyrosine 817, not tyrosine 515, was increased in placentas from obese women. Maternal obesity was associated with increased phosphorylation of MAPK p38 in placentas from male fetuses, but not phosphorylation of ERK p42/44. BDNF regulation is complex and highly regulated. Pre-pregnancy/early maternal obesity adversely affects BDNF/TRKB signaling in the placenta in a sexually dimorphic manner. These data collectively suggest that induction of placental TRKB signaling could ameliorate the placental OB phenotype, thus improving perinatal outcome. Copyright © 2016 Elsevier Ltd. All rights reserved.

  11. Platelets Drive Thrombus Propagation in a Hematocrit and Glycoprotein VI-Dependent Manner in an In Vitro Venous Thrombosis Model.

    Science.gov (United States)

    Lehmann, Marcus; Schoeman, Rogier M; Krohl, Patrick J; Wallbank, Alison M; Samaniuk, Joseph R; Jandrot-Perrus, Martine; Neeves, Keith B

    2018-05-01

    The objective of this study was to measure the role of platelets and red blood cells on thrombus propagation in an in vitro model of venous valvular stasis. A microfluidic model with dimensional similarity to human venous valves consists of a sinus distal to a sudden expansion, where for sufficiently high Reynolds numbers, 2 countercurrent vortices arise because of flow separation. The primary vortex is defined by the points of flow separation and reattachment. A secondary vortex forms in the deepest recess of the valve pocket characterized by low shear rates. An initial fibrin gel formed within the secondary vortex of a tissue factor-coated valve sinus. Platelets accumulated at the interface of the fibrin gel and the primary vortex. Red blood cells at physiological hematocrits were necessary to provide an adequate flux of platelets to support thrombus growth out of the valve sinus. A subpopulation of platelets that adhered to fibrin expose phosphatidylserine. Platelet-dependent thrombus growth was attenuated by inhibition of glycoprotein VI with a blocking Fab fragment or D-dimer. A 3-step process regulated by hemodynamics was necessary for robust thrombus propagation: First, immobilized tissue factor initiates coagulation and fibrin deposition within a low flow niche defined by a secondary vortex in the pocket of a model venous valve. Second, a primary vortex delivers platelets to the fibrin interface in a red blood cell-dependent manner. Third, platelets adhere to fibrin, activate through glycoprotein VI, express phosphatidylserine, and subsequently promote thrombus growth beyond the valve sinus and into the bulk flow. © 2018 American Heart Association, Inc.

  12. Cortical thinning in the anterior cingulate cortex predicts multiple sclerosis patients' fluency performance in a lateralised manner

    Directory of Open Access Journals (Sweden)

    Olivia Geisseler

    2016-01-01

    Full Text Available Cognitive impairment is as an important feature of Multiple Sclerosis (MS, and might be even more relevant to patients than mobility restrictions. Compared to the multitude of studies investigating memory deficits or basic cognitive slowing, executive dysfunction is a rarely studied cognitive domain in MS, and its neural correlates remain largely unexplored. Even rarer are topological studies on specific cognitive functions in MS. Here we used several structural MRI parameters – including cortical thinning and T2 lesion load – to investigate neural correlates of executive dysfunction, both on a global and a regional level by means of voxel- and vertex-wise analyses. Forty-eight patients with relapsing-remitting MS and 48 healthy controls participated in the study. Five executive functions were assessed, i.e. verbal and figural fluency, working memory, interference control and set shifting. Patients scored lower than controls in verbal and figural fluency only, and displayed widespread cortical thinning. On a global level, cortical thickness independently predicted verbal fluency performance, when controlling for lesion volume and central brain atrophy estimates. On a regional level, cortical thinning in the anterior cingulate region correlated with deficits in verbal and figural fluency and did so in a lateralised manner: Left-sided thinning was related to reduced verbal – but not figural – fluency, whereas the opposite pattern was observed for right-sided thinning. We conclude that executive dysfunction in MS patients can specifically affect verbal and figural fluency. The observed lateralised clinico-anatomical correlation has previously been described in brain-damaged patients with large focal lesions only, for example after stroke. Based on focal grey matter atrophy, we here show for the first time comparable lateralised findings in a white matter disease with widespread pathology.

  13. GTP depletion synergizes the anti-proliferative activity of chemotherapeutic agents in a cell type-dependent manner

    International Nuclear Information System (INIS)

    Lin, Tao; Meng, Lingjun; Tsai, Robert Y.L.

    2011-01-01

    Highlights: → Strong synergy between mycophenolic acid (MPA) and 5-FU in MDA-MB-231 cells. → Cell type-dependent synergy between MPA and anti-proliferative agents. → The synergy of MPA on 5-FU is recapitulated by RNA polymerase-I inhibition. → The synergy of MPA on 5-FU requires the expression of nucleostemin. -- Abstract: Mycophenolic acid (MPA) depletes intracellular GTP by blocking de novo guanine nucleotide synthesis. GTP is used ubiquitously for DNA/RNA synthesis and as a signaling molecule. Here, we made a surprising discovery that the anti-proliferative activity of MPA acts synergistically with specific chemotherapeutic agents in a cell type-dependent manner. In MDA-MB-231 cells, MPA shows an extremely potent synergy with 5-FU but not with doxorubicin or etoposide. The synergy between 5-FU and MPA works most effectively against the highly tumorigenic mammary tumor cells compared to the less tumorigenic ones, and does not work in the non-breast cancer cell types that we tested, with the exception of PC3 cells. On the contrary, MPA shows the highest synergy with paclitaxel but not with 5-FU in SCC-25 cells, derived from oral squamous cell carcinomas. Mechanistically, the synergistic effect of MPA on 5-FU in MDA-MB-231 cells can be recapitulated by inhibiting the RNA polymerase-I activity and requires the expression of nucleostemin. This work reveals that the synergy between MPA and anti-proliferative agents is determined by cell type-dependent factors.

  14. Dihydrocapsaicin (DHC), a saturated structural analog of capsaicin, induces autophagy in human cancer cells in a catalase-regulated manner.

    Science.gov (United States)

    Oh, Seon Hee; Kim, Young Soon; Lim, Sung Chul; Hou, Yi Feng; Chang, In Youb; You, Ho Jin

    2008-11-01

    Although capsaicin, a pungent component of red pepper, is known to induce apoptosis in several types of cancer cells, the mechanisms underlying capsaicin-induced cytotoxicity are unclear. Here, we showed that dihydrocapsaicin (DHC), an analog of capsaicin, is a potential inducer of autophagy. DHC was more cytotoxic than capsaicin in HCT116, MCF-7 and WI38 cell lines. Capsaicin and DHC did not affect the sub-G(1) apoptotic peak, but induced G(0)/G(1) arrest in HCT116 and MCF-7 cells. DHC caused the artificial autophagosome marker GFP-LC3 to redistribute and upregulated expression of autophagy-related proteins. Blocking of autophagy by 3-methyladenine (3MA) as well as siRNA Atg5 induced a high level of caspase-3 activation. Although pretreatment with zVAD completely inhibited caspase-3 activation by 3MA, it did not prevent cell death. DHC-induced autophagy was enhanced by zVAD pretreatment, as shown by increased accumulation of LC3-II protein. DHC attenuated basal ROS levels through catalase induction; this effect was enhanced by antioxidants, which increased both LC3-II expression and caspase-3 activation. The catalase inhibitor 3-amino-1,2,4-triazole (3AT) abrogated DHC-induced expression of LC3-II, overexpression of the catalase gene increased expression of LC3-II protein, and knockdown decreased it. Additionally, DHC-induced autophagy was independent of p53 status. Collectively, DHC activates autophagy in a p53-independent manner and that may contribute to cytotoxicity of DHC.

  15. Ketogenic diets improve behaviors associated with autism spectrum disorder in a sex-specific manner in the EL mouse.

    Science.gov (United States)

    Ruskin, David N; Fortin, Jessica A; Bisnauth, Subrina N; Masino, Susan A

    2017-01-01

    The core symptoms of autism spectrum disorder are poorly treated with current medications. Symptoms of autism spectrum disorder are frequently comorbid with a diagnosis of epilepsy and vice versa. Medically-supervised ketogenic diets are remarkably effective nonpharmacological treatments for epilepsy, even in drug-refractory cases. There is accumulating evidence that supports the efficacy of ketogenic diets in treating the core symptoms of autism spectrum disorders in animal models as well as limited reports of benefits in patients. This study tests the behavioral effects of ketogenic diet feeding in the EL mouse, a model with behavioral characteristics of autism spectrum disorder and comorbid epilepsy. Male and female EL mice were fed control diet or one of two ketogenic diet formulas ad libitum starting at 5weeks of age. Beginning at 8weeks of age, diet protocols continued and performance of each group on tests of sociability and repetitive behavior was assessed. A ketogenic diet improved behavioral characteristics of autism spectrum disorder in a sex- and test-specific manner; ketogenic diet never worsened relevant behaviors. Ketogenic diet feeding improved multiple measures of sociability and reduced repetitive behavior in female mice, with limited effects in males. Additional experiments in female mice showed that a less strict, more clinically-relevant diet formula was equally effective in improving sociability and reducing repetitive behavior. Taken together these results add to the growing number of studies suggesting that ketogenic and related diets may provide significant relief from the core symptoms of autism spectrum disorder, and suggest that in some cases there may be increased efficacy in females. Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.

  16. Letrozole regulates actin cytoskeleton polymerization dynamics in a SRC-1 dependent manner in the hippocampus of mice.

    Science.gov (United States)

    Zhao, Yangang; Yu, Yanlan; Zhang, Yuanyuan; He, Li; Qiu, Linli; Zhao, Jikai; Liu, Mengying; Zhang, Jiqiang

    2017-03-01

    In the hippocampus, local estrogens (E 2 ) derived from testosterone that is catalyzed by aromatase play important roles in the regulation of hippocampal neural plasticity, but the underlying mechanisms remain unclear. The actin cytoskeleton contributes greatly to hippocampal synaptic plasticity; however, whether it is regulated by local E 2 and the related mechanisms remain to be elucidated. In this study, we first examined the postnatal developmental profiles of hippocampal aromatase and specific proteins responsible for actin cytoskeleton dynamics. Then we used aromatase inhibitor letrozole (LET) to block local E 2 synthesis and examined the changes of these proteins and steroid receptor coactivator-1 (SRC-1), the predominant coactivator for steroid nuclear receptors. Finally, SRC-1 specific RNA interference was used to examine the effects of SRC-1 on the expression of these actin remodeling proteins. The results showed a V-type profile for aromatase and increased profiles for actin cytoskeleton proteins in both male and female hippocampus without obvious sex differences. LET treatment dramatically decreased the F-actin/G-actin ratio, the expression of Rictor, phospho-AKT (ser473), Profilin-1, phospho-Cofilin (Ser3), and SRC-1 in a dose-dependent manner. In vitro studies demonstrated that LET induced downregulation of these proteins could be reversed by E 2 , and E 2 induced increase of these proteins were significantly suppressed by SRC-1 shRNA interference. These results for the first time clearly demonstrated that local E 2 inhibition could induce aberrant actin polymerization; they also showed an important role of SRC-1 in the mediation of local E 2 action on hippocampal synaptic plasticity by regulation of actin cytoskeleton dynamics. Copyright © 2016 Elsevier Ltd. All rights reserved.

  17. UV-induced nuclear import of XPA is mediated by importin-α4 in an ATR-dependent manner.

    Directory of Open Access Journals (Sweden)

    Zhengke Li

    Full Text Available Xeroderma pigmentosum Group A (XPA is a crucial factor in mammalian nucleotide excision repair (NER and nuclear import of XPA from the cytoplasm for NER is regulated in cellular DNA damage responses in S-phase. In this study, experiments were carried out to determine the transport mechanisms that are responsible for the UV (ultraviolet-induced nuclear import of XPA. We found that, in addition to the nuclear localization signal (NLS of XPA, importin-α4 or/and importin-α7 are required for the XPA nuclear import. Further investigation indicated that, importin-α4 and importin-α7 directly interacted with XPA in cells. Interestingly, the binding of importin-α4 to XPA was dependent on UV-irradiation, while the binding of importin-α7 was not, suggesting a role for importin-α7 in nuclear translocation of XPA in the absence of DNA damage, perhaps with specificity to certain non-S-phases of the cell-cycle. Consistent with the previous report of a dependence of UV-induced XPA nuclear import on ataxia telangiectasia and Rad3-related protein (ATR in S-phase, knockdown of ATR reduced the amount of XPA interacting with importin-α4. In contrast, the GTPase XPA binding protein 1 (XAB1, previously proposed to be required for XPA nuclear import, showed no effect on the nuclear import of XPA in our siRNA knockdown analysis. In conclusion, our results suggest that upon DNA damage transport adaptor importin-α4 imports XPA into the nucleus in an ATR-dependent manner, while XAB1 has no role in this process. In addition, these findings reveal a potential new therapeutic target for the sensitization of cancer cells to chemotherapy.

  18. Knockdown of the fat mass and obesity gene disrupts cellular energy balance in a cell-type specific manner.

    Directory of Open Access Journals (Sweden)

    Ryan T Pitman

    Full Text Available Recent studies suggest that FTO variants strongly correlate with obesity and mainly influence energy intake with little effect on the basal metabolic rate. We suggest that FTO influences eating behavior by modulating intracellular energy levels and downstream signaling mechanisms which control energy intake and metabolism. Since FTO plays a particularly important role in adipocytes and in hypothalamic neurons, SH-SY5Y neuronal cells and 3T3-L1 adipocytes were used to understand how siRNA mediated knockdown of FTO expression alters cellular energy homeostasis. Cellular energy status was evaluated by measuring ATP levels using a luminescence assay and uptake of fluorescent glucose. FTO siRNA in SH-SY5Y cells mediated mRNA knockdown (-82%, increased ATP concentrations by up to 46% (P = 0.013 compared to controls, and decreased phosphorylation of AMPk and Akt in SH-SY5Y by -52% and -46% respectively as seen by immunoblotting. In contrast, FTO siRNA in 3T3-L1 cells decreased ATP concentration by -93% (p<0.0005, and increased AMPk and Akt phosphorylation by 204% and 70%, respectively suggesting that FTO mediates control of energy levels in a cell-type specific manner. Furthermore, glucose uptake was decreased in both SH-SY5Y (-51% p = 0.015 and 3T3-L1 cells (-30%, p = 0.0002. We also show that FTO knockdown decreases NPY mRNA expression in SH-SY5Y cells (-21% through upregulation of pSTAT3 (118%. These results provide important evidence that FTO-variant linked obesity may be associated with altered metabolic functions through activation of downstream metabolic mediators including AMPk.

  19. Important interactional strategies for everyday public health nursing practice.

    Science.gov (United States)

    Porr, Caroline J

    2015-01-01

    This Clinical Concepts article concerns the relational tools required by public health nurses to establish relationships with single mothers living on public assistance, mothers who are vulnerable and often stigmatized. The implications of stigmatization for relationship building are highlighted based on previous research investigating how public health nurses working in Canadian jurisdictions establish professional caring relationships with this cohort of mothers. Public health nurses employed interactional strategies including engaging in a positive manner and offering verbal commendations which served as effective relational tools to break through mothers' walls of defensiveness and to resume the dynamic process of relationship building. Building Relationship is a key practice standard for public health nurses and is instrumental to their work at both individual and community levels to improve social determinants of health. The author concludes with recommendations to facilitate building relationships during everyday public health nursing practice. © 2013 Wiley Periodicals, Inc.

  20. Public water supply sources - the practical problems

    International Nuclear Information System (INIS)

    Chambers, E.G.W.

    1990-01-01

    A complex system of reservoirs, streams, treatment works and pipe networks is used to provide the public water supply to consumers in Strathclyde. The manner in which a nuclear event would affect the quality of water available from this supply would depend on a wide variety of factors. The extent to which the quality from each source could be maintained or improved if found to be unsatisfactory would depend on the extent of contamination and the particular characteristics of each source. Development of contingency plans will incorporate monitoring of supplies and development of effective communications both internally and externally. (author)

  1. Public Relations in the Public Sector

    DEFF Research Database (Denmark)

    Valentini, Chiara

    2013-01-01

    the findings. The study also suffers from the limits of a quantitative research approach, which provides less elaborate accounts of public communication officers’ perceptions of the strategic role of communication in the public sector. Originality/value of paper: This study contributes to the existing......Purpose of the paper: The aim of this study is to examine the level of strategic management of public administrations and information and communication programs by public communication officers in Italy. Methodology: The study is quantitative in nature. It was constructed according to the findings...... of the Excellence Project in Public Relations and thus applies a research instrument similar to that used in other international scholarly investigations in public relations. The population comprises civil servants working in information and communication activities for Italian public administrations. Findings...

  2. FINANCING PUBLIC TRANSPORT SERVICES FROM PUBLIC FUNDS

    Directory of Open Access Journals (Sweden)

    Miloš POLIAK

    2017-12-01

    Full Text Available The paper deals with the issue of efficiency of public passenger transport through financial support from public funds from the perspective of improving road safety. The aim is to verify the hypothesis that financing public passenger transport from public funds is a significant tool to influence the number of passengers carried by individual automobile transport, and thus it can be used a tool for influencing road safety in a particular territory. The first part of the paper analyses the sources for financial support of public passenger transport. The next part describes the assumptions for improving road safety through increasing the support of public passenger transport. The last part analyses possible impacts of financing public passenger transport on the road safety in relation to the specified hypothesis.

  3. Public procurement process

    African Journals Online (AJOL)

    NESG Policy Brief: April 2017. Public ... transparency in public procurement; and greater concerns about efficiency, fairness, and equity. ... government enacted the Public Procurement Act (2007) [1] which provides legislative framework for.

  4. Public Health Departments

    Data.gov (United States)

    Department of Homeland Security — State and Local Public Health Departments in the United States Governmental public health departments are responsible for creating and maintaining conditions that...

  5. Public Value Dimensions

    DEFF Research Database (Denmark)

    Andersen, lotte bøgh; Beck Jørgensen, Torben; Kjeldsen, Anne-Mette

    2012-01-01

    Further integration of the public value literature with other strands of literature within Public Administration necessitates a more specific classification of public values. This paper applies a typology linked to organizational design principles, because this is useful for empirical public...... administration studies. Based on an existing typology of modes of governance, we develop a classification and test it empirically, using survey data from a study of the values of 501 public managers. We distinguish between seven value dimensions (the public at large, rule abidance, societal interests, budget...... the integration between the public value literature and other parts of the Public Administration discipline....

  6. Immortalisation with hTERT Impacts on Sulphated Glycosaminoglycan Secretion and Immunophenotype in a Variable and Cell Specific Manner.

    Directory of Open Access Journals (Sweden)

    Tina P Dale

    differentiation potential, was affected in a variable manner. As such, these cells are not a direct substitute for primary cells in cartilage regeneration research.

  7. Human decidual stromal cells secrete soluble pro-apoptotic factors during decidualization in a cAMP-dependent manner.

    Science.gov (United States)

    Leno-Durán, E; Ruiz-Magaña, M J; Muñoz-Fernández, R; Requena, F; Olivares, E G; Ruiz-Ruiz, C

    2014-10-10

    Is there a relationship between decidualization and apoptosis of decidual stromal cells (DSC)? Decidualization triggers the secretion of soluble factors that induce apoptosis in DSC. The differentiation and apoptosis of DSC during decidualization of the receptive decidua are crucial processes for the controlled invasion of trophoblasts in normal pregnancy. Most DSC regress in a time-dependent manner, and their removal is important to provide space for the embryo to grow. However, the mechanism that controls DSC death is poorly understood. The apoptotic response of DSC was analyzed after exposure to different exogenous agents and during decidualization. The apoptotic potential of decidualized DSC supernatants and prolactin (PRL) was also evaluated. DSC lines were established from samples of decidua from first trimester pregnancies. Apoptosis was assayed by flow cytometry. PRL production, as a marker of decidualization, was determined by enzyme-linked immunosorbent assay. DSCs were resistant to a variety of apoptosis-inducing substances. Nevertheless, DSC underwent apoptosis during decidualization in culture, with cAMP being essential for both apoptosis and differentiation. In addition, culture supernatants from decidualized DSC induced apoptosis in undifferentiated DSC, although paradoxically these supernatants decreased the spontaneous apoptosis of decidual lymphocytes. Exogenously added PRL did not induce apoptosis in DSC and an antibody that neutralized the PRL receptor did not decrease the apoptosis induced by supernatants. Further studies are needed to examine the involvement of other soluble factors secreted by decidualized DSC in the induction of apoptosis. The present results indicate that apoptosis of DSC occurs in parallel to differentiation, in response to decidualization signals, with soluble factors secreted by decidualized DSC being responsible for triggering cell death. These studies are relevant in the understanding of how the regression of decidua

  8. Cardiac myosin binding protein C phosphorylation affects cross-bridge cycle's elementary steps in a site-specific manner.

    Directory of Open Access Journals (Sweden)

    Li Wang

    Full Text Available Based on our recent finding that cardiac myosin binding protein C (cMyBP-C phosphorylation affects muscle contractility in a site-specific manner, we further studied the force per cross-bridge and the kinetic constants of the elementary steps in the six-state cross-bridge model in cMyBP-C mutated transgenic mice for better understanding of the influence of cMyBP-C phosphorylation on contractile functions. Papillary muscle fibres were dissected from cMyBP-C mutated mice of ADA (Ala273-Asp282-Ala302, DAD (Asp273-Ala282-Asp302, SAS (Ser273-Ala282-Ser302, and t/t (cMyBP-C null genotypes, and the results were compared to transgenic mice expressing wide-type (WT cMyBP-C. Sinusoidal analyses were performed with serial concentrations of ATP, phosphate (Pi, and ADP. Both t/t and DAD mutants significantly reduced active tension, force per cross-bridge, apparent rate constant (2πc, and the rate constant of cross-bridge detachment. In contrast to the weakened ATP binding and enhanced Pi and ADP release steps in t/t mice, DAD mice showed a decreased ADP release without affecting the ATP binding and the Pi release. ADA showed decreased ADP release, and slightly increased ATP binding and cross-bridge detachment steps, whereas SAS diminished the ATP binding step and accelerated the ADP release step. t/t has the broadest effects with changes in most elementary steps of the cross-bridge cycle, DAD mimics t/t to a large extent, and ADA and SAS predominantly affect the nucleotide binding steps. We conclude that the reduced tension production in DAD and t/t is the result of reduced force per cross-bridge, instead of the less number of strongly attached cross-bridges. We further conclude that cMyBP-C is an allosteric activator of myosin to increase cross-bridge force, and its phosphorylation status modulates the force, which is regulated by variety of protein kinases.

  9. Prenatal stress programs neuroendocrine stress responses and affective behaviors in second generation rats in a sex-dependent manner.

    Science.gov (United States)

    Grundwald, Natalia J; Brunton, Paula J

    2015-12-01

    with controls, with no differences in the F2 females. No differences in depressive-like behavior (sucrose preference or forced swim test) were observed in either sex. In conclusion, the effects of maternal stress during pregnancy on HPA axis regulation and anxiety-like behavior can be transmitted to future generations in a sex-dependent manner. These data have implications for human neuropsychiatric disorders with developmental origins. Copyright © 2015 The Authors. Published by Elsevier Ltd.. All rights reserved.

  10. Smurf2 E3 ubiquitin ligase modulates proliferation and invasiveness of breast cancer cells in a CNKSR2 dependent manner.

    Science.gov (United States)

    David, Diana; Jagadeeshan, Sankar; Hariharan, Ramkumar; Nair, Asha Sivakumari; Pillai, Radhakrishna Madhavan

    2014-01-01

    Smurf2 is a member of the HECT family of E3 ubiquitin ligases that play important roles in determining the competence of cells to respond to TGF- β/BMP signaling pathway. However, besides TGF-β/BMP pathway, Smurf2 regulates a repertoire of other signaling pathways ranging from planar cell polarity during embryonic development to cell proliferation, migration, differentiation and senescence. Expression of Smurf2 is found to be dysregulated in many cancers including breast cancer. The purpose of the present study is to examine the effect of Smurf2 knockdown on the tumorigenic potential of human breast cancer cells emphasizing more on proliferative signaling pathway. siRNAs targeting different regions of the Smurf2 mRNA were employed to knockdown the expression of Smurf2. The biological effects of synthetic siRNAs on human breast cancer cells were investigated by examining the cell proliferation, migration, invasion, focus formation, anchorage-independent growth, cell cycle arrest, and cell cycle and cell proliferation related protein expressions upon Smurf2 silencing. Smurf2 silencing in human breast cancer cells resulted in a decreased focus formation potential and clonogenicity as well as in vitro cell migration/invasion capabilities. Moreover, knockdown of Smurf2 suppressed cell proliferation. Cell cycle analysis showed that the anti-proliferative effect of Smurf2 siRNA was mediated by arresting cells in the G0/G1 phase, which was caused by decreased expression of cyclin D1and cdk4, followed by upregulation p21 and p27. Furthermore, we demonstrated that silencing of Smurf2 downregulated the proliferation of breast cancer cells by modulating the PI3K- PTEN-AKT-FoxO3a pathway via the scaffold protein CNKSR2 which is involved in RAS-dependent signaling pathways. The present study provides the first evidence that silencing Smurf2 using synthetic siRNAs can regulate the tumorigenic properties of human breast cancer cells in a CNKSR2 dependent manner. Our results

  11. Global regulator SATB1 recruits beta-catenin and regulates T(H2 differentiation in Wnt-dependent manner.

    Directory of Open Access Journals (Sweden)

    Dimple Notani

    2010-01-01

    Full Text Available In vertebrates, the conserved Wnt signalling cascade promotes the stabilization and nuclear accumulation of beta-catenin, which then associates with the lymphoid enhancer factor/T cell factor proteins (LEF/TCFs to activate target genes. Wnt/beta -catenin signalling is essential for T cell development and differentiation. Here we show that special AT-rich binding protein 1 (SATB1, the T lineage-enriched chromatin organizer and global regulator, interacts with beta-catenin and recruits it to SATB1's genomic binding sites. Gene expression profiling revealed that the genes repressed by SATB1 are upregulated upon Wnt signalling. Competition between SATB1 and TCF affects the transcription of TCF-regulated genes upon beta-catenin signalling. GATA-3 is a T helper type 2 (T(H2 specific transcription factor that regulates production of T(H2 cytokines and functions as T(H2 lineage determinant. SATB1 positively regulated GATA-3 and siRNA-mediated knockdown of SATB1 downregulated GATA-3 expression in differentiating human CD4(+ T cells, suggesting that SATB1 influences T(H2 lineage commitment by reprogramming gene expression. In the presence of Dickkopf 1 (Dkk1, an inhibitor of Wnt signalling, GATA-3 is downregulated and the expression of signature T(H2 cytokines such as IL-4, IL-10, and IL-13 is reduced, indicating that Wnt signalling is essential for T(H2 differentiation. Knockdown of beta-catenin also produced similar results, confirming the role of Wnt/beta-catenin signalling in T(H2 differentiation. Furthermore, chromatin immunoprecipitation analysis revealed that SATB1 recruits beta-catenin and p300 acetyltransferase on GATA-3 promoter in differentiating T(H2 cells in a Wnt-dependent manner. SATB1 coordinates T(H2 lineage commitment by reprogramming gene expression. The SATB1:beta-catenin complex activates a number of SATB1 regulated genes, and hence this study has potential to find novel Wnt responsive genes. These results demonstrate that SATB1

  12. Iron-Restricted Diet Affects Brain Ferritin Levels, Dopamine Metabolism and Cellular Prion Protein in a Region-Specific Manner

    Directory of Open Access Journals (Sweden)

    Jessica M. V. Pino

    2017-05-01

    disorders. Our findings show that nutritional iron deficiency produces these molecular alterations in a region-specific manner and provide new insight into the variety of molecular pathways that can lead to distinct neurological symptoms upon iron deficiency. Thus, adequate iron supplementation is essential for brain health and prevention of neurological diseases.

  13. Argon inhalation attenuates retinal apoptosis after ischemia/reperfusion injury in a time- and dose-dependent manner in rats.

    Directory of Open Access Journals (Sweden)

    Felix Ulbrich

    Full Text Available Retinal ischemia and reperfusion injuries (IRI permanently affect neuronal tissue and function by apoptosis and inflammation due to the limited regenerative potential of neurons. Recently, evidence emerged that the noble gas Argon exerts protective properties, while lacking any detrimental or adverse effects. We hypothesized that Argon inhalation after IRI would exert antiapoptotic effects in the retina, thereby protecting retinal ganglion cells (RGC of the rat's eye.IRI was performed on the left eyes of rats (n = 8 with or without inhaled Argon postconditioning (25, 50 and 75 Vol% for 1 hour immediately or delayed after ischemia (i.e. 1.5 and 3 hours. Retinal tissue was harvested after 24 hours to analyze mRNA and protein expression of Bcl-2, Bax and Caspase-3, NF-κB. Densities of fluorogold-prelabeled RGCs were analyzed 7 days after injury in whole-mounts. Histological tissue samples were prepared for immunohistochemistry and blood was analyzed regarding systemic effects of Argon or IRI. Statistics were performed using One-Way ANOVA.IRI induced RGC loss was reduced by Argon 75 Vol% inhalation and was dose-dependently attenuated by lower concentrations, or by delayed Argon inhalation (1504±300 vs. 2761±257; p<0.001. Moreover, Argon inhibited Bax and Bcl-2 mRNA expression significantly (Bax: 1.64±0.30 vs. 0.78±0.29 and Bcl-2: 2.07±0.29 vs. 0.99±0.22; both p<0.01, as well as caspase-3 cleavage (1.91±0.46 vs. 1.05±0.36; p<0.001. Expression of NF-κB was attenuated significantly. Immunohistochemistry revealed an affection of Müller cells and astrocytes. In addition, IRI induced leukocytosis was reduced significantly after Argon inhalation at 75 Vol%.Immediate and delayed Argon postconditioning protects IRI induced apoptotic loss of RGC in a time- and dose-dependent manner, possibly mediated by the inhibition of NF-κB. Further studies need to evaluate Argon's possible role as a therapeutic option.

  14. Synaptic dysbindin-1 reductions in schizophrenia occur in an isoform-specific manner indicating their subsynaptic location.

    Directory of Open Access Journals (Sweden)

    Konrad Talbot

    Full Text Available BACKGROUND: An increasing number of studies report associations between variation in DTNBP1, a top candidate gene in schizophrenia, and both the clinical symptoms of the disorder and its cognitive deficits. DTNBP1 encodes dysbindin-1, reduced levels of which have been found in synaptic fields of schizophrenia cases. This study determined whether such synaptic reductions are isoform-specific. METHODOLOGY/PRINCIPAL FINDINGS: Using Western blotting of tissue fractions, we first determined the synaptic localization of the three major dysbindin-1 isoforms (A, B, and C. All three were concentrated in synaptosomes of multiple brain areas, including auditory association cortices in the posterior half of the superior temporal gyrus (pSTG and the hippocampal formation (HF. Tests on the subsynaptic tissue fractions revealed that each isoform is predominantly, if not exclusively, associated with synaptic vesicles (dysbindin-1B or with postsynaptic densities (dysbindin-1A and -1C. Using Western blotting on pSTG (n = 15 and HF (n = 15 synaptosomal fractions from schizophrenia cases and their matched controls, we discovered that synaptic dysbindin-1 is reduced in an isoform-specific manner in schizophrenia without changes in levels of synaptophysin or PSD-95. In pSTG, about 92% of the schizophrenia cases displayed synaptic dysbindin-1A reductions averaging 48% (p = 0.0007 without alterations in other dysbindin-1 isoforms. In the HF, by contrast, schizophrenia cases displayed normal levels of synaptic dysbindin-1A, but 67% showed synaptic reductions in dysbindin-1B averaging 33% (p = 0.0256, while 80% showed synaptic reductions in dysbindin-1C averaging 35% (p = 0.0171. CONCLUSIONS/SIGNIFICANCE: Given the distinctive subsynaptic localization of dysbindin-1A, -1B, and -1C across brain regions, the observed pSTG reductions in dysbindin-1A are postsynaptic and may promote dendritic spine loss with consequent disruption of auditory information

  15. Sorafenib modulates the radio sensitivity of hepatocellular carcinoma cells in vitro in a schedule-dependent manner

    International Nuclear Information System (INIS)

    Li, Qiaoqiao; Hu, Yonghong; Xi, Mian; He, Liru; Zhao, Lei; Liu, Mengzhong

    2012-01-01

    Hepatocellular carcinoma (HCC) has a high incidence and mortality. Radiotherapy and sorafenib have proven effective for HCC. Here, we investigated whether sorafenib modulated the response of HCC cells to irradiation in vitro, effect of timing of sorafenib, and the underlying mechanisms. Cell viability of the HCC cell lines, SMMC-7721 and Bel-7402, was examined by the 3-(4,5-dimethylthiazol-2-yl)-5(3-carboxymethoxyphenyl)-2(4-sulfophenyl)-2 H-terazolium (MTT) assays. Clonogenic growth assays of SMMC-7721 and Bel-7402 were determined by colony formation assays. DNA damage was assessed by monitoring γ-HAX foci in irradiated cells with immunofluorescence microscopy, and cell cycle distribution changes were examined by flow cytometry. Effects of sorafenib (15 μM) added 30 min prior to radiation (pre-irradiation sorafenib) of SMMC-7721 and BEL-7402 or 24 h post-irradiation (post-irradiation sorafenib) on irradiated SMMC-7721 and BEL-7402 cells were compared to those of radiation alone or no treatment. The effect of sorafenib was dependent on its time of addition in relationship to irradiation of cells. Pre-irradiation sorafenib did not significantly affect the viability of SMMC-7221 and BEL-7402 cells compared with irradiation treatment alone. In contrast, post-irradiation sorafenib increased the sensitivity of irradiated SMMC-7221 and BEL-7402 cells significantly in a time-dependent manner. Pre-irradiation sorafenib significantly increased the surviving fraction of SMMC-7221 and BEL-7402 cells in clonogenic assays whereas post-irradiation sorafenib significantly reduced the surviving fractions of SMMC-7221 and BEL-7402 cells. SMMC-7721 cells treated with sorafenib 30 min before irradiation had significantly fewer cells with γ-H2AX foci (23.8 ± 2.9%) than SMMC-7721 cells receiving radiation alone (59.9 ± 2.4; P < 0.001). Similarly, BEL-7402 cells receiving sorafenib prior to irradiation had significantly fewer cells with γ-H2AX foci (46.4 ± 3.8%) than those

  16. Sorafenib modulates the radio sensitivity of hepatocellular carcinoma cells in vitro in a schedule-dependent manner

    Directory of Open Access Journals (Sweden)

    Li Qiaoqiao

    2012-10-01

    Full Text Available Abstract Background Hepatocellular carcinoma (HCC has a high incidence and mortality. Radiotherapy and sorafenib have proven effective for HCC. Here, we investigated whether sorafenib modulated the response of HCC cells to irradiation in vitro, effect of timing of sorafenib, and the underlying mechanisms. Methods Cell viability of the HCC cell lines, SMMC-7721 and Bel-7402, was examined by the 3-(4,5-dimethylthiazol-2-yl-5(3-carboxymethoxyphenyl-2(4-sulfophenyl-2 H-terazolium (MTT assays. Clonogenic growth assays of SMMC-7721 and Bel-7402 were determined by colony formation assays. DNA damage was assessed by monitoring γ-HAX foci in irradiated cells with immunofluorescence microscopy, and cell cycle distribution changes were examined by flow cytometry. Effects of sorafenib (15 μM added 30 min prior to radiation (pre-irradiation sorafenib of SMMC-7721 and BEL-7402 or 24 h post-irradiation (post-irradiation sorafenib on irradiated SMMC-7721 and BEL-7402 cells were compared to those of radiation alone or no treatment. Results The effect of sorafenib was dependent on its time of addition in relationship to irradiation of cells. Pre-irradiation sorafenib did not significantly affect the viability of SMMC-7221 and BEL-7402 cells compared with irradiation treatment alone. In contrast, post-irradiation sorafenib increased the sensitivity of irradiated SMMC-7221 and BEL-7402 cells significantly in a time-dependent manner. Pre-irradiation sorafenib significantly increased the surviving fraction of SMMC-7221 and BEL-7402 cells in clonogenic assays whereas post-irradiation sorafenib significantly reduced the surviving fractions of SMMC-7221 and BEL-7402 cells. SMMC-7721 cells treated with sorafenib 30 min before irradiation had significantly fewer cells with γ-H2AX foci (23.8 ± 2.9% than SMMC-7721 cells receiving radiation alone (59.9 ± 2.4; P  Conclusions Sorafenib combined with irradiation exerted a schedule-dependent effect in

  17. From networked publics to issue publics

    DEFF Research Database (Denmark)

    Birkbak, Andreas

    2013-01-01

    As an increasing part of everyday life becomes connected with the web in many areas of the globe, the question of how the web mediates political processes becomes still more urgent. Several scholars have started to address this question by thinking about the web in terms of a public space....... In this paper, we aim to make a twofold contribution towards the development of the concept of publics in web science. First, we propose that although the notion of publics raises a variety of issues, two major concerns continue to be user privacy and democratic citizenship on the web. Well-known arguments hold......, this paper points towards an alternative way to think about publics by proposing a pragmatist reorientation of the public/private distinction in web science, away from seeing two spheres that needs to be kept separate, towards seeing the public and the private as something that is continuously connected...

  18. Publication ethics in public health emergencies.

    Science.gov (United States)

    Shaw, David; Elger, Bernice S

    2017-09-01

    In this article, we describe and analyse three issues in publication ethics that are raised when conducting research in emergencies and disasters. These include reluctance to share data and samples because of concerns about publications, loss of individual authorship in high high-profile multi-entity publications, and the deaths of authors during dangerous research projects. An emergency research pledge may be useful in avoiding some of these issues. © The Author 2016. Published by Oxford University Press on behalf of Faculty of Public Health. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  19. Public relations effectiveness in public health institutions.

    Science.gov (United States)

    Springston, Jeffrey K; Weaver Lariscy, Ruth Ann

    2005-01-01

    This article explores public relations effectiveness in public health institutions. First, the two major elements that comprise public relations effectiveness are discussed: reputation management and stakeholder relations. The factors that define effective reputation management are examined, as are the roles of issues and crisis management in building and maintaining reputation. The article also examines the major facets of stakeholder relations, including an inventory of stakeholder linkages and key audiences, such as the media. Finally, methods of evaluating public relations effectiveness at both the program level and the institutional level are explored.

  20. Acyl-CoA-binding protein (ACBP) localizes to the endoplasmic reticulum and Golgi in a ligand-dependent manner in mammalian cells

    DEFF Research Database (Denmark)

    Hansen, Jesper S; Færgeman, Nils J; Kragelund, Birthe B

    2008-01-01

    showed that ACBP targeted to the ER (endoplasmic reticulum) and Golgi in a ligand-binding-dependent manner. A variant Y28F/K32A-FACI-50, which is unable to bind acyl-CoA, did no longer show association with the ER and became segregated from the Golgi, as analysed by intensity correlation calculations....... Depletion of fatty acids from cells by addition of FAFBSA (fatty-acid-free BSA) significantly decreased FACI-50 association with the Golgi, whereas fatty acid overloading increased Golgi association, strongly supporting that ACBP associates with the Golgi in a ligand-dependent manner. FRAP (fluorescence...... recovery after photobleaching) showed that the fatty-acid-induced targeting of FACI-50 to the Golgi resulted in a 5-fold reduction in FACI-50 mobility. We suggest that ACBP is targeted to the ER and Golgi in a ligand-binding-dependent manner in living cells and propose that ACBP may be involved...

  1. Public Entrepreneurship and Innovation

    OpenAIRE

    Roberts, Nancy

    1992-01-01

    This article presents a conceptual framework of public entrepreneurship. Public entrepreneurship is defined as the generation of a novel or innovative idea and the design and implementation of the innovative idea into public sector practice. The conceptual framework is used to distinguish between public entrepreneurs and other actors in the policy process, and to clarify the differences between policy, political, executive, and bureaucratic entrepreneurs....

  2. Public Relations and Marketing.

    Science.gov (United States)

    Savage, Daniel D.

    1987-01-01

    Urges community colleges to adopt pro-active public relations strategies. Examines the role of the public information officer in such areas as coordination of public relations and marketing activities, relations with media, and the development of a comprehensive public relations plan. (AYC)

  3. Public private partnerships

    NARCIS (Netherlands)

    Miranda Sarmento, J.J.

    2014-01-01

    Public-private partnerships (PPPs) are increasing in number worldwide and are used to build and manage large public infrastructure projects. In PPPs, the private sector plays a role in developing and maintaining public infrastructure and services, which is usually a public sector responsibility.

  4. From Public International to International Public Law: Translating World Public Opinion into International Public Authority

    NARCIS (Netherlands)

    von Bogdandy, A.; Goldmann, M.; Venzke, I.

    This article argues that increasing demands in world public opinion for legitimate and effective international institutions require a paradigm shift in public international law. There is a part of public international law that should be better understood as international public law because it

  5. Achieving Public Schools

    Science.gov (United States)

    Abowitz, Kathleen Knight

    2011-01-01

    Public schools are functionally provided through structural arrangements such as government funding, but public schools are achieved in substance, in part, through local governance. In this essay, Kathleen Knight Abowitz explains the bifocal nature of achieving public schools; that is, that schools are both subject to the unitary Public compact of…

  6. A Powerful Public Sphere?

    DEFF Research Database (Denmark)

    Fiig, Christina

    The paper holds a critical discussion of the Habermasian model of the public sphere and proposes a revised model of a general public......The paper holds a critical discussion of the Habermasian model of the public sphere and proposes a revised model of a general public...

  7. A Study about Youth and Uses of Public Spaces

    Directory of Open Access Journals (Sweden)

    Fardin Alikhah

    2016-04-01

    Full Text Available There has been a huge concern about the manner of confrontations of different social groups with urban public spaces within urban scholars. Among these social groups, young people have been particularly important. Because they have a tangible presence in the city's public spaces and social life of the city are affected by their presence. This paper examines the uses of public spaces by young people and will pay special attention to the role of social control on use of public spaces. Paper focuses on the study of young people who attend in public places with their friends from opposite sex. We have inspired by theories of urban public spaces such as Oldenburg's third place as well as comprehensive research of Rob White on crime, policing and urban public spaces in Australia in this paper. The main question of the paper is that this particular group of young people choose which public spaces and why? In a qualitative approach, two techniques of observation and in - depth interviews have been chosen for collecting data. Original data collected in interviews with 20 girls and boys who attend in public places with their friends from opposite sex. Results show that parks and coffee shops are preferred urban public places of youth. Formal control would push these youth to out of the way and cozy public spaces.

  8. The public information imperative

    International Nuclear Information System (INIS)

    Copeland, S.

    2001-01-01

    Public approval hinges not only on delivering the information the public wants but on providing tangible evidence that we are listening to public concerns. We must respond. Public acceptance depends on making real change which speaks to people's concerns. The message that the public wants to hear is that government are listening and acting on what they hear. In Canada, the nuclear regulator is increasingly active in the public arena. We held cross-country consultations as we prepared Canada's strong new Act and regulations. We have developed information vehicles such as the Radiation Index and our web site. We continue to extensively involve the public in our licensing process. All licensing hearings are open to the public. Nothing is harder to capture than public trust. This conference marks a substantial investment in learning and in our common future. We can work to build our credibility as regulators who acts on public concerns. (N.C.)

  9. Ascorbic acid alters cell fate commitment of human neural progenitors in a WNT/β-catenin/ROS signaling dependent manner.

    Science.gov (United States)

    Rharass, Tareck; Lantow, Margareta; Gbankoto, Adam; Weiss, Dieter G; Panáková, Daniela; Lucas, Stéphanie

    2017-10-16

    increased WNT/β-catenin signaling output i.e. MYC mRNA level, whereas RuR attenuated it. Moreover, AA improved neurogenesis as much as LiCl as both TUBB3-positive cell yield and TUBB3 mRNA level increased, while NAC or RuR attenuated neurogenesis. Markedly, the neurogenesis outputs between the short and the full treatment with either NAC or AA were found unchanged, supporting our model that neuronal yield is altered by events taking place at the early phase of differentiation. Our findings demonstrate that AA treatment elevates ROS metabolism in a non-lethal manner prior to the NPCs commitment to their neuronal fate. Such effect stimulates the redox-sensitive DVL2 activation and WNT/β-catenin signaling response that would enhance the ensuing neuronal cell differentiation.

  10. Public accounting and its challenges: the case of Hungary

    Directory of Open Access Journals (Sweden)

    JOZSEF SIMON

    2013-09-01

    Full Text Available In a democratic state, citizens expect that those currently in power be obliged to justify the way they spend and manage public funds. The enforcement of accountability has several preconditions. At the same time, the accounting system constitutes one part of the public sector’s information system. Thus, studying the information system in a systematic manner is important. The information system must meet diverse needs. The present paper aims at showing those requirements. When considered in a comparative perspective, different public accountancy systems implemented internationally show different forms. Every country can decide in which area and how the cash-flow approach and accrual based accounting can be used. The purpose of this paper is to verify whether and how could the usage of accrual based accounting influence the public accounting methods and the functions of public accountancy.

  11. Audit Report "Department of Energy Efforts to Manage Information Technology Resources in an Energy-Efficient and Environmentally Responsible Manner"

    Energy Technology Data Exchange (ETDEWEB)

    None

    2009-05-01

    necessary steps to reduce energy consumption and resource usage of their data centers, such as identifying and monitoring the amount of energy used at their facilities. We concluded that Headquarters programs offices (which are part of the Department of Energy's Common Operating Environment) as well as field sites had not developed and/or implemented policies and procedures necessary to ensure that information technology equipment and supporting infrastructure was operated in an energy-efficient manner and in a way that minimized impact on the environment. For example, although required by the Department, sites had not enabled computer equipment power management features designed to reduce energy consumption. In addition, officials within Headquarters programs and at the sites reviewed had not effectively monitored performance or taken steps to fully evaluate available reductions in energy usage at their facilities. Without improvements, the Department will not be able to take advantage of opportunities to reduce energy consumption and realize cost savings of nearly $23 million over the next five years at just the seven sites reviewed. We noted that the potential for reduced energy consumption at these sites alone was equivalent to the annual power requirements of over 2,400 homes or, alternatively, removing about 3,000 cars from the road each year. Many of the available energy reduction strategies, such as fully utilizing energy-efficient settings on the many computers used by the Department and its contractors, are 'low hanging fruit' in that they will provide immediate tangible energy savings at little or no cost. Others, such as a shift to thin-client computing, an environment that transfers the processing capabilities from an individual's desk to a shared server environment, will require some level of investment which can, based on available literature, be successfully recovered through reduced acquisition and support costs. In our judgment, given

  12. 26 CFR 301.9100-7T - Time and manner of making certain elections under the Tax Reform Act of 1986.

    Science.gov (United States)

    2010-04-01

    ... trust under the Internal Revenue Code if such entity was created in 1906 as a common law trust and governed by the trust laws of the State of Minnesota, receives royalties from iron ore leases, and income... General Rules Application of Internal Revenue Laws § 301.9100-7T Time and manner of making certain...

  13. 29 CFR 102.114 - Filing and service of papers by parties; form of papers; manner and proof of filing or service...

    Science.gov (United States)

    2010-07-01

    ..., or by registered mail, certified mail, regular mail, electronic mail (if the document was filed... proceeding, the other party shall be served by electronic mail (e-mail), if possible. If the other party does...; manner and proof of filing or service; electronic filings. 102.114 Section 102.114 Labor Regulations...

  14. Discretion and Public Benefit in a Regulatory Agency

    OpenAIRE

    Nagarajan, Vijaya

    2013-01-01

    This book explores the manner in which a variety of public benefits such as environmental protection and consumer safety have been accommodated through the authorisation process within competition law and policy in Australia. While the regulator’s use of its discretion can be explained as a triumph of practice over theory, this book explores the potential for competition principles to be imbued by the wider discourses of democratic participation and human rights. In doing so it makes a signif...

  15. Behavioral Public Administration

    DEFF Research Database (Denmark)

    Grimmelikhuijsen, Stephan; Jilke, Sebastian; Olsen, Asmus Leth

    2017-01-01

    on theories and methods from psychology and related fields and point to research in public administration that could benefit from further integration. An analysis of public administration topics through a psychological lens can be useful to confirm, add nuance to, or extend classical public administration...... theories. As such, behavioral public administration complements traditional public administration. Furthermore, it could be a two-way street for psychologists who want to test the external validity of their theories in a political-administrative setting. Finally, four principles are proposed to narrow......Behavioral public administration is the analysis of public administration from the micro-level perspective of individual behavior and attitudes by drawing on insights from psychology on the behavior of individuals and groups. The authors discuss how scholars in public administration currently draw...

  16. Group Work Publication-1991.

    Science.gov (United States)

    Zimpfer, David G.

    1992-01-01

    Lists 21 new publications in group work, of which 9 are reviewed. Those discussed include publications on group counseling and psychotherapy, structured groups, support groups, psychodrama, and social group work. (Author/NB)

  17. Challenges to Public Health

    Indian Academy of Sciences (India)

    First page Back Continue Last page Graphics. Challenges to Public Health. Tracing of the infection. Isolation of patients to stop spread. Laboratory diagnosis. Hospitalization &Treatment. Stock pile & supply of drugs. Planning & mitigation. Information to public. Support to SEARO countries.

  18. Public Service Motivation

    Directory of Open Access Journals (Sweden)

    Raluca-Marilena Mihalcioiu

    2011-05-01

    Full Text Available Public Service Motivation concept was developed in North America and focuses on specific motivations of public servants, such as employee satisfaction, organizational commitment, reward preferences, organizational and individual performance. Other types of motivation, as financial consideration, are relevant but have less important influences with regard to this kind of work outcomes. This strengthen the assertion for a diversified motivational strategy, which affect various types of motivation, while not losing sight of the public value that one organization shows and therefore valuing public service motivation as a specific contribution to work outcomes. The concept has been increasingly applied in European public administration. This paper presents Status Quo of international Public Service Motivation research and locates in them empirical evidences from contries that are already working with this concept, like Austria. It also analyses implications for central questions of public management. The main focus of this article is general appropriateness and possible applications for Romanian public management research.

  19. Lighting and public health.

    NARCIS (Netherlands)

    Ierland, J. van & Schreuder, D.A.

    1969-01-01

    The following topics; are discussed with respect to public health: - the effect of visible and ultraviolet radiation upon man. - vision with respect to lighting. interior lighting. - artificial lighting of work environments. - day light and windows. - recommendations for lighting. public lighting. -

  20. Public Key Infrastructure Study

    National Research Council Canada - National Science Library

    Berkovits, Shimshon

    1994-01-01

    The National Institute of Standards and Technology (NIST) has tasked The MITRE Corporation to study the alternatives for automated management of public keys and of the associated public key certificates for the Federal Government...

  1. 40 CFR 720.45 - Information that must be included in the notice form.

    Science.gov (United States)

    2010-07-01

    ... manufactured, processed, or used. (2) A process description of each manufacture, processing, and use operation which includes a diagram of the major unit operations and chemical conversions, the identity and entry... composition that can be represented by a specific, complete chemical structure diagram (a Class 1 substance...

  2. Public informations guidelines

    International Nuclear Information System (INIS)

    1986-06-01

    The purpose of these Public Information Guidelines is to provide principles for the implementation of the NWPA mandate and the Mission Plan requirements for the provision of public information. These Guidelines set forth the public information policy to be followed by all Office of Civilian Radioactive Waste Management (OCRWM) performance components. The OCRWM offices should observe these Guidelines in shaping and conducting public information activities

  3. Public and proud

    DEFF Research Database (Denmark)

    Schwartz, Sander Andreas

    2017-01-01

    This paper is a qualitative study that examines how and why some citizens use their Facebook network as a personal public. The concept of the personal public in this study is defined by a relative sense of privacy in the closed individual Facebook network, together with a sense of publicness based...... public political communication that have so far been understudied in research on political debates on Facebook....

  4. Public Sector Organizations

    DEFF Research Database (Denmark)

    Meyer, Renate; Leixnering, Stephan

    2015-01-01

    New public management-inspired reforms created numerous autonomous units with many different faces and labels. This variety of organizations and organizational forms precludes a straightforward definition of what constitutes a public sector organization and blurs the boundaries between the public...... and private sectors as well as the boundaries of single organizations. In addition, the complexity of the interlocking arrangements and relationships in this public organizational landscape has resulted in considerable governance problems with serious implications for coordination and policy coherence....

  5. Public informations guidelines

    Energy Technology Data Exchange (ETDEWEB)

    None

    1986-06-01

    The purpose of these Public Information Guidelines is to provide principles for the implementation of the NWPA mandate and the Mission Plan requirements for the provision of public information. These Guidelines set forth the public information policy to be followed by all Office of Civilian Radioactive Waste Management (OCRWM) performance components. The OCRWM offices should observe these Guidelines in shaping and conducting public information activities.

  6. Productivity in Public Services

    OpenAIRE

    Helen Simpson

    2007-01-01

    This paper discusses issues arising in the measurement of productivity in public services. Compared to measuring productivity in the private sector difficulties arise because the output of public services is often un-priced and because some public services are consumed collectively. A key problem is measuring the full range of outputs and quality improvements delivered by public sector organisations that are valued by society. Without comprehensive measures of output productivity statistics m...

  7. Public preferences for government spending in Canada

    Directory of Open Access Journals (Sweden)

    Ramji Sabrina

    2012-10-01

    Full Text Available Abstract This study considers three questions: 1. What are the Canadian public’s prioritization preferences for new government spending on a range of public health-related goods outside the scope of the country’s national system of health insurance? 2. How homogenous or heterogeneous is the Canadian public in terms of these preferences? 3. What factors are predictive of the Canadian public’s preferences for new government spending? Data were collected in 2008 from a national random sample of Canadian adults through a telephone interview survey (n =1,005. Respondents were asked to rank five spending priorities in terms of their preference for new government spending. Bivariate and multivariable logistic regression analyses were conducted. As a first priority, Canadian adults prefer spending on child care (26.2%, followed by pharmacare (23.1%, dental care (20.8%, home care (17.2%, and vision care (12.7%. Sociodemographic characteristics predict spending preferences, based on the social position and needs of respondents. Policy leaders need to give fair consideration to public preferences in priority setting approaches in order to ensure that public health-related goods are distributed in a manner that best suits population needs.

  8. Effect of nuclear education on public attitude

    International Nuclear Information System (INIS)

    Ohnishi, Teruaki

    1995-01-01

    A method is proposed to assess the effect of nuclear education. In this method, the nuclear education is treated as a part of the activities for public acceptance (PA), and a unit PA activity is assumed to give the same effect on the public, in essence, as a unit of nuclear information given by the newsmedia. Moreover, the change of attitude to nuclear energy is assumed to originate from enhanced understanding which, in turn, is brought by the stimulus given by the nuclear education. With the values of constants determined by using the data in Japan, example calculations were made for the educational time b 0 and the infiltration rate of education into minors B as parameters. It became clear from this calculation that the attitude to nuclear energy formed in the age of school children plays an essential role in shaping future public opinion since it is held in individuals without any notable modification for a long time after its formation, and that the effect of nuclear education to minors emerges depending on the variables b 0 and B in a highly non-linear manner. It was also found that there exists an optimum condition for nuclear education to attain the maximum amelioration of public opinion under a given condition of man-power for educational workers. (author)

  9. EIA publications directory, 1992

    International Nuclear Information System (INIS)

    1993-01-01

    This directory contains abstracts and ordering information for EIA publications. The abstracts are arranged by broad subject category such as coal, petroleum, natural gas, and electric power. A comprehensive subject index, a title index, and a report number index are included. Each entry gives the title, report number, publication frequency, date, number of pages, and ordering information. Publication began with the 1979 edition

  10. Effective Public Management

    Science.gov (United States)

    Bower, Joseph L.

    1977-01-01

    Argues that public management differs from private management not just in degree but in quality, so that American business is an inappropriate analogy for evaluating public management. In particular, "purpose,""organization," and "people" have different meaning and significance in public agencies and private businesses. (JG)

  11. ABCC publication manual

    Energy Technology Data Exchange (ETDEWEB)

    1959-01-01

    The reporting policy of the Atomic Bomb Casualty Commission is described in detail in this manual. Specific topics covered are: research project register; research publication policy; USA publication procedures; Japan publication procedures, report preparation instructions; and a glossary of institutions terms, phrases, and symbols. 15 figures. (DMC)

  12. Public perception of nanotechnology

    International Nuclear Information System (INIS)

    Burri, Regula Valerie; Bellucci, Sergio

    2008-01-01

    While several studies on the public opinion of nanotechnology have pointed to a rather enthusiastic U.S. public, the public uptake of nanotechnology in Europe is more contained. The results of the Swiss publifocus on nanotechnology reveal a pragmatic attitude of citizens toward the emerging technologies, thus confirming what has been identified as a 'balanced approach' in the NanoJury UK

  13. Selected DOE headquarters publications

    International Nuclear Information System (INIS)

    1979-04-01

    This publication provides listings of (mainly policy and programmatic) publications which have been issued by headquarters organizations of the Department of Energy; assigned a DOE/XXX- type report number code, where XXX is the 1- to 4-letter code for the issuing headquarters organization; received by the Energy Library; and made available to the public

  14. Proactive Public Disclosure

    DEFF Research Database (Denmark)

    Boll, Karen; Tell, Michael

    2015-01-01

    This article discusses proactive public disclosure of taxpayer information and how this may form a new strategy for securing tax compliance by tax administrators. It reports a case study from the Danish Customs and Tax Administration in which consumers of services–over a short period of time...... proactive public disclosure is compatible with the Duty of Confidentiality, but incompatible with Good Public Governance. Furthermore, the analyses show that there are a number of strong organizational rationales for using proactive public disclosure, despite its apparent incompatibility with Good Public...... Governance. The article is innovative in that it combines a legal and organizational approach to analyse a new regulatory strategy within tax administration....

  15. Public Participation Plan

    International Nuclear Information System (INIS)

    1993-07-01

    The purpose of this Public Participation Plan is to describe the US Department of Energy's (DOE) plan for involving the public in the decision-making process for the Uranium Mill Tailings Remedial Action (UMTRA) Project. The plan describes how the DOE will meet the public participation requirements of the Uranium Mill Tailings Radiation Control Act (UMTRCA) of 1978, as amended, and of the National Environmental Policy Act (NEPA) of 1969. It includes the UMTRA Project Office plans for complying with DOE Order 5440.1D and for implementing the DOE's Public Participation Policy for Environmental Restoration and Waste Management (1992) and Public Participation Guidance for Environmental Restoration and Waste Management (1993)

  16. Public education programme for nuclear contingency planning in Hong Kong

    International Nuclear Information System (INIS)

    Wong, M. C.; Li, S. W.

    2002-01-01

    Two nuclear power stations on the coast of southern China are situated some 50 kilometers to the northeast of Hong Kong. Although the stations are far away from Hong Kong, the construction and operation of the nuclear power stations have generated public anxiety locally, in particular, after the Chernobyl accident in 1986. A comprehensive contingency plan which takes into account such concerns of the public has been implemented in Hong Kong. Cooperation by the public is vital to the effective implementation of any contingency plan. Understanding of the basics of radiation protection as well as the contingency plan will help the public to appreciate the situation and react in a rational manner. A public education program to promote awareness of the contingency plan has been implemented in Hong Kong. In particular, a Virtual Exhibition Hall on radiation has been developed and launched in February 2002 for access by the public via Internet. A video and a set of web pages will be launched in the later part of 2002 to inform and educate the public on matters related to nuclear accident response in Hong Kong. This paper describes the public education programme in Hong Kong to promote public awareness and understanding of the nuclear contingency plan

  17. Barriers to public participation

    International Nuclear Information System (INIS)

    Patten, E.

    1986-01-01

    When one talks of public involvement, one immediately thinks of a public hearing. Six problems with the public hearing system are listed and discussed. The constraints involved in the public hearing process are: 1) asking for technical information from general folks; 2) overwhelming the public with complex information; 3) having a format that intimidates the speaker and allows no opportunity for useful dialogue; 4) obtaining a skewed picture of an issue because one hasn't had truly representative comments; 5) citizens having overblown expectations of what public involvement means, and becoming frustrated and cynical when their advice isn't heeded; and 6) convincing the powers that be that a comprehensive program is a wise investment. The second half of this paper lists 21 other forms of public participation, giving a description of each form, its purpose, and brief comments on its advantages

  18. Cutaneous T cell lymphoma expresses immunosuppressive CD80 (B7-1) cell surface protein in a STAT5-dependent manner

    DEFF Research Database (Denmark)

    Zhang, Qian; Wang, Hong Yi; Wei, Fang

    2014-01-01

    as their joint ability to transcriptionally activate the CD80 gene. In IL-2-dependent CTCL cells, CD80 expression is induced by the cytokine in a Jak1/3- and STAT5a/b-dependent manner, whereas in the CTCL cells with constitutive STAT5 activation, CD80 expression is also STAT5a/b dependent but is independent......(+) and CD8(+) populations or the CD4(+) subset alone, transfected with CD152 mRNA, inhibits proliferation of normal T cells in a CD152- and CD80-dependent manner. These data identify a new mechanism of immune evasion in CTCL and suggest that the CD80-CD152 axis may become a therapeutic target in this type...

  19. An endocrine disruptor, bisphenol A, affects development in the protochordate Ciona intestinalis: Hatching rates and swimming behavior alter in a dose-dependent manner

    International Nuclear Information System (INIS)

    Matsushima, Ayami; Ryan, Kerrianne; Shimohigashi, Yasuyuki; Meinertzhagen, Ian A.

    2013-01-01

    Bisphenol A (BPA) is widely used industrially to produce polycarbonate plastics and epoxy resins. Numerous studies document the harmful effects caused by low-dose BPA exposure especially on nervous systems and behavior in experimental animals such as mice and rats. Here, we exposed embryos of a model chordate, Ciona intestinalis, to seawater containing BPA to evaluate adverse effects on embryonic development and on the swimming behavior of subsequent larvae. Ciona is ideal because its larva develops rapidly and has few cells. The rate of larval hatching decreased in a dose-dependent manner with exposures to BPA above 3 μM; swimming behavior was also affected in larvae emerging from embryos exposed to 1 μM BPA. Adverse effects were most severe on fertilized eggs exposed to BPA within 7 h post-fertilization. Ciona shares twelve nuclear receptors with mammals, and BPA is proposed to disturb the physiological functions of one or more of these. - Highlights: ► Embryos of Ciona intestinalis were exposed to BPA to evaluate its developmental effects. ► The rate of larval hatching decreased in a dose-dependent manner. ► Swimming behavior was affected in larvae that emerge from embryos exposed to 1 μM BPA. ► Our findings will support a new strategy to analyze the developmental effects induced by BPA. - Exposure of fertilized Ciona embryos to BPA decreased their hatch rate in a dose-dependent manner and led to abnormal larval swimming behavior.

  20. Public Information Plan

    International Nuclear Information System (INIS)

    1984-06-01

    The Public Information Plan is intended to be used in conjunction with the DOE-UMTRA Project ''Public Participation Plan'' to describe the Department of Energy's plan for involving the public in the decision-making process related to the Uranium Mill Tailings Remedial Action (UMTRA) Project. This project was authorized by Congress in the Uranium Mill Tailings Radiation Control of Act of 1978, PL95-604. The Act provides for a cooperative effort with affected states and Indian tribes for the cleanup of designated abandoned or inactive uranium mill tailings sites. The objective of the Public Information Plan of the UMTRA Project is timely and sufficient dissemination of factual information to promote understanding of the project by federal, state, and local officials, the media, special interest groups, and the general public; and thereby to encourage informed participation in the project by the public and government officials. The Uranium Mill Tailings Radiation Control Act provides for public involvement in remedial action planning, with special consideration given to landowners, Indian tribes, and the states. According to the Act, the Secretary of Energy shall hold public hearings in the states where processing sites, vicinity properties, and disposal sites are located. Public participation in the UMTRA Project will not, however, be limited to those mechanisms formally required by law. The public may also be involved informally through informational meetings, workshops, and local citizens' task forces. 12 refs., 2 figs., 1 tab

  1. Modele de management și implicațiile acestora asupra comunicării publice

    Directory of Open Access Journals (Sweden)

    Corina RĂDULESCU

    2016-06-01

    Full Text Available Each management model leads to substantial changes at the level of public communication. In our approach we highlight the gradual openness of the public institution towards the general public, first considered to be a simple administered, then a client (New Public Management and, currently, a collaborator in a partnership manner (democratic governance. Alongside, we notice the shift from a limited communication to a highly developed one. Unlike the bureaucratic model, which is based on the supereminence of the law, and places the publicity principle on a secondary place, newer New Public Management and democratic governance models invert the ratio, complying the administration with the needs expressed by the citizen. Consequently, to meet the challenges of the new models, communication activities face an unprecedented momentum, and the citizen must partake in an increasingly manner in conceiving administrative action, up to the involvement in the budgetary process.

  2. BUDGET AND PUBLIC DEBT

    Directory of Open Access Journals (Sweden)

    Morar Ioan Dan

    2014-12-01

    Full Text Available The issue of public budgeting is an important issue for public policy of the state, for the simple reason that no money from the state budget can not promote public policy. Budgetary policy is official government Doctrine vision mirror and also represents a starting point for other public policies, which in turn are financed by the public budget. Fiscal policy instruments at its disposal handles the public sector in its structure, and the private sector. Tools such as grant, budgetary allocation, tax, welfare under various forms, direct investments and not least the state aid is used by the state through their budgetary policies to directly and indirectly infuence sector, and the private. Fiscal policies can be grouped according to the structure of the public sector in these components, namely fiscal policy, budgeting and resource allocation policies for financing the budget deficit. An important issue is the financing of the budget deficit budgetary policies. There are two funding possibilities, namely, the higher taxes or more axles site and enter the second call to public loans. Both options involve extra effort from taxpayers in the current fiscal year when they pay higher taxes or a future period when public loans will be repaid. We know that by virtue of "fiscal pact" structural deficits of the member countries of the EU are limited by the European Commission, according to the macro structural stability and budget of each Member State. This problem tempers to some extent the governments of the Member States budgetary appetite, but does not solve the problem of chronic budget deficits. Another issue addressed in this paper is related to the public debt, the absolute amount of its relative level of public datoriri, about the size of GDP, public debt financing and its repayment sources. Sources of public debt issuance and monetary impact on the budget and monetary stability are variables that must underpin the justification of budgetary

  3. DOE headquarters publications

    International Nuclear Information System (INIS)

    1978-12-01

    This bibliography provides listings of (mainly policy and programmatic) publications issued from the U.S. Department of Energy, Washington, D.C. The listings are arranged by the report number assigned to each publication. All of the publications listed, except for those shown as still in preparation, may be seen in the Energy Library. A title index arranged by title keywords follows the listings. Certain publications have been omitted. They include such items as pamphlets, fact sheets, bulletins and weekly/monthly issuances of DOE's Energy Information Administration and Economic Regulatory Administration, and employee bulletins and newsletters. Omitted from the bibliography altogether are headquarters publications assigned other types of report codes--e.g., HCP (Headquarters Contractor Publication) and CONF

  4. Publication point indicators

    DEFF Research Database (Denmark)

    Elleby, Anita; Ingwersen, Peter

    2010-01-01

    ; the Cumulated Publication Point Indicator (CPPI), which graphically illustrates the cumulated gain of obtained vs. ideal points, both seen as vectors; and the normalized Cumulated Publication Point Index (nCPPI) that represents the cumulated gain of publication success as index values, either graphically......The paper presents comparative analyses of two publication point systems, The Norwegian and the in-house system from the interdisciplinary Danish Institute of International Studies (DIIS), used as case in the study for publications published 2006, and compares central citation-based indicators...... with novel publication point indicators (PPIs) that are formalized and exemplified. Two diachronic citation windows are applied: 2006-07 and 2006-08. Web of Science (WoS) as well as Google Scholar (GS) are applied to observe the cite delay and citedness for the different document types published by DIIS...

  5. Pigs in Public Health

    DEFF Research Database (Denmark)

    Svendsen, Mette N.

    2017-01-01

    of public health, made me re-evaluate both what ‘public’ and what ‘health’ means in public health. In this commentary I provide a short personal account of that intellectual journey. I argue that entanglements between species make it urgent that public health scholars investigate the moral, socio......Animals are rare topics in public health science texts and speech despite the fact that animal bodies and lives are woven into the health of human populations, and vice versa. Years of ethnographic and documentary research – following pigs and their humans in and out of biomedical research – made......-economic, material, and bacterial passages between humans and animals that constitute the various publics of public health and profoundly shape the health of human and animal populations in a globalized world....

  6. PUBLIC POLICY AND TAXATION

    Directory of Open Access Journals (Sweden)

    IOSIF MOLDOVAN

    2014-10-01

    Full Text Available The state administration process and hence also the economy coordination effort requires the promotion of robust, consistent and transparent public policy, which must be accepted by all stakeholders of economic development. Public policy is a set of measures taken by the authorities legally constituted as public power. Under normal conditions these policy aims at improving living conditions of citizens by developing grounded strategies which are applied by measures implemented to stimulate economic development in all its complexity by harmonizing the efforts of the institutional and non-institutional bodies responsible for ensuring the overall public interest. In Romania, public policies, especially fiscal ones on which we dwell, not reached in many cases the expected effects primarily because of their superficial grounding, lack of transparency, unpredictability, poor communication and secondly as an effect of ineffective management of public financial resources.

  7. Public perceptions of risk

    International Nuclear Information System (INIS)

    Mann, J.M.

    1983-01-01

    Public perceptions of risk will probably always be unsatisfying to a scientist. Public perceptions are formed by the actions of institutions which have goals other than formation of an informed public opinion, such as the schools, the media, business and the government. In this environment, it seems unrealistic to expect public opinion to reflect scientific realities. The talk will focus on the media as an opinion-former and will discuss several non-nuclear issues as illustrations: plague in New Mexico, Toxic Shock Syndrome, and Injuries as a Public Health Problem. Ultimately however, we are confronted with two wonderfully complex matters: can risk be adequately expressed or measured in universally comprehensible or broadly acceptable terms; and the mysterious movements of the public and collective mind

  8. From Public Relations to Corporate Public Diplomacy

    DEFF Research Database (Denmark)

    Mogensen, Kirsten

    2017-01-01

    This paper illustrates several factors that make corporate public diplomacy a fundamentally different approach to activities that aim at legitimacy alone. A case study of a suspended Chinese hydropower project (i.e., the Myitsone Dam) in northern Myanmar is presented to address the functional...

  9. Scientists: Engage the Public!

    OpenAIRE

    Shugart, Erika C.; Racaniello, Vincent R.

    2015-01-01

    ABSTRACT Scientists must communicate about science with public audiences to promote an understanding of complex issues that we face in our technologically advanced society. Some scientists may be concerned about a social stigma or ?Sagan effect? associated with participating in public communication. Recent research in the social sciences indicates that public communication by scientists is not a niche activity but is widely done and can be beneficial to a scientist?s career. There are a varie...

  10. Financing Public Service Broadcasting

    DEFF Research Database (Denmark)

    Berg, Christian Edelvold; Lund, Anker Brink

    2012-01-01

    Broadcasting (PSB) financing regimes in Europe, concluding that Denmark, Finland, Iceland, Norway, and Sweden may still be considered conventional, licence fee PSB countries, but with some interesting differences in relation to competitive and market oriented alternatives of resource provision......Recently several European countries have abolished the traditional public service licence fee system, replacing it with direct public funding. But except for Iceland, the Nordic countries have not followed suit. The article discusses this development within a comparative framework of Public Service...

  11. Strategi Komunikasi Public Relations

    OpenAIRE

    Artis, Artis

    2011-01-01

    Living man in interrelates society. Relationship among human being done by gets communication so man one by another one mutually understand and influence regard for the benefit, it that always been practiced by Public Relations( liaison) in a governance and also firm institute to reach to the effect which ices. The institute of Public Relations declares for,”Public Relations is overall effort which be passed off by design and berkesenambungan in order to creates and pet keenness and mutual un...

  12. Public health and Plowshare

    Energy Technology Data Exchange (ETDEWEB)

    Terrill, Jr, J G [Consumer Protection and Environmental Health Service, U.S. PubIic Health Service, Washington, DC (United States)

    1969-07-01

    The protection of public health and safety is a principal area of concern in any application of nuclear energy. A health and safety analysis must be conducted and reviewed by appropriate agencies and the final results made available to interested agencies and groups, both public and private, prior to the application. This is especially important for the Plowshare Program - the peaceful uses of nuclear explosives - where the public is to be the ultimate beneficiary. Because public health must be a primary concern in the Plowshare Program, it is essential that the potential risk be weighed against the expected benefits to the public. Public health agencies must play an increasingly important role in the planning and operational stages of the peaceful applications of nuclear explosives and in the final stage of consumer use of Plowshare-generated products. There are many long term and long distance ramifications of the Plowshare Program, such a the potential radiological contamination of consumer products that may reach the consumer at long times after the event or at great distances from the site of the event. Criteria for evaluating public exposure to radiation from these products need to be developed based on sound scientific research. Standards for radioactivity in consumer products must be developed in relation to potential exposure of the public. Above all, a clear benefit to the public with a minimum of risk must be shown. The major purpose of this Symposium on the Public Health Aspects of Peaceful Uses of Nuclear-Explosives is to focus attention on the health and safety aspects, present the results of safety analyses accomplished to date and other information necessary to an understanding of the public health aspects, and to identify areas where additional research is required. A general overview of the total symposium content is presented with emphasis on the relationship of the topics to public health. (author)

  13. Public health and Plowshare

    International Nuclear Information System (INIS)

    Terrill, J.G. Jr.

    1969-01-01

    The protection of public health and safety is a principal area of concern in any application of nuclear energy. A health and safety analysis must be conducted and reviewed by appropriate agencies and the final results made available to interested agencies and groups, both public and private, prior to the application. This is especially important for the Plowshare Program - the peaceful uses of nuclear explosives - where the public is to be the ultimate beneficiary. Because public health must be a primary concern in the Plowshare Program, it is essential that the potential risk be weighed against the expected benefits to the public. Public health agencies must play an increasingly important role in the planning and operational stages of the peaceful applications of nuclear explosives and in the final stage of consumer use of Plowshare-generated products. There are many long term and long distance ramifications of the Plowshare Program, such a the potential radiological contamination of consumer products that may reach the consumer at long times after the event or at great distances from the site of the event. Criteria for evaluating public exposure to radiation from these products need to be developed based on sound scientific research. Standards for radioactivity in consumer products must be developed in relation to potential exposure of the public. Above all, a clear benefit to the public with a minimum of risk must be shown. The major purpose of this Symposium on the Public Health Aspects of Peaceful Uses of Nuclear-Explosives is to focus attention on the health and safety aspects, present the results of safety analyses accomplished to date and other information necessary to an understanding of the public health aspects, and to identify areas where additional research is required. A general overview of the total symposium content is presented with emphasis on the relationship of the topics to public health. (author)

  14. Transportation and public health.

    Science.gov (United States)

    Litman, Todd

    2013-01-01

    This article investigates various ways that transportation policy and planning decisions affect public health and better ways to incorporate public health objectives into transport planning. Conventional planning tends to consider some public health impacts, such as crash risk and pollution emissions measured per vehicle-kilometer, but generally ignores health problems resulting from less active transport (reduced walking and cycling activity) and the additional crashes and pollution caused by increased vehicle mileage. As a result, transport agencies tend to undervalue strategies that increase transport system diversity and reduce vehicle travel. This article identifies various win-win strategies that can help improve public health and other planning objectives.

  15. Environmental Public Health Tracking

    Centers for Disease Control (CDC) Podcasts

    In this podcast series, CDC scientists address frequently asked questions about the National Environmental Public Health Tracking Network, including using and applying data, running queries, and much more.

  16. List of publications 1987

    International Nuclear Information System (INIS)

    1988-05-01

    This annual bibliography of publications from University of Oslo, Department of physics refers to research reports, contribution to newspapers and scientific journals, guest lectures, and popular science lectures

  17. Performance in Public Organizations

    DEFF Research Database (Denmark)

    Andersen, Lotte Bøgh; Boesen, Andreas; Pedersen, Lene Holm

    2016-01-01

    of management and performance are classified. The results illustrate how a systematization of the conceptual space of performance in public organizations can help researchers select what to study and what to leave out with greater accuracy while also bringing greater clarity to public debates about performance.......Performance in public organizations is a key concept that requires clarification. Based on a conceptual review of research published in 10 public administration journals, this article proposes six distinctions to describe the systematic differences in performance criteria: From which stakeholder...

  18. Public Procurement and Innovation

    DEFF Research Database (Denmark)

    Rolfstam, Max

    be understood as a special case of innovation, where social processes – and consequently the institutions governing these social processes – need to be considered. This book contributes to our understanding with a detailed institutional analysis of the public procurement of innovation.......Max Rolfstam examines the increasing emphasis on public procurement as a means to stimulate innovation and the theoretical implications of this policy development. While ‘regular’ public procurement may be regarded as the outcome of anonymous market processes, public procurement of innovation must...

  19. Air University Press Publications

    National Research Council Canada - National Science Library

    2002-01-01

    .... Our publications program is designed primarily to help Air Force and other US war fighters, national leaders and policy makers, academicians, military historians, and other analysts understand...

  20. CDBG Public Services Activity

    Data.gov (United States)

    Department of Housing and Urban Development — CDBG activity related to public services, including senior services, legal services, youth services, employment training, health services, homebuyer counseling, food...

  1. Public Relations vs. Propaganda

    Directory of Open Access Journals (Sweden)

    Alexandru BASTIAN

    2006-10-01

    Full Text Available The paper aims at presenting the characteristics, methods and aplications of two related activities – Public Relations and propaganda. Although different from the piont of wiev of purpose and results (the practice of Public Relations aims at establishing and maintaining mutual lines of communications, understanding, acceptance, and cooperation between an organization and its publics, through transparency and honesty, while propaganda insists on a message that is intended primarily to serve the interests of the messenger. in order to influence public opinion and to manipulate other people’s beliefs by any means necessary, the two activities also present quite a lot of similarities.

  2. Public power costs less

    International Nuclear Information System (INIS)

    Moody, D.

    1993-01-01

    The reasons why residential customers of public power utilities paid less for power than private sector customers is discussed. Residential customers of investor-owned utilities (IOU's) paid average rates that were 28% above those paid by customers by possibly owned systems during 1990. The reasons for this disparity are that management costs faced by public power systems are below those of private power companies, indicating a greater efficiency of management among public power systems, and customer accounts expenses averaged $33.00 per customer for publicly owned electric utilities compared to $39.00 per customer for private utilities

  3. Enhanced Publications Linking Publications and Research Data in Digital Repositories

    CERN Document Server

    Vernooy-Gerritsen, Marjan

    2009-01-01

    The traditional publication will be overhauled by the 'Enhanced Publication'. This is a publication that is enhanced with research data, extra materials, post publication data, and database records. It has an object-based structure with explicit l

  4. Mars Public Mapping Project: Public Participation in Science Research; Providing Opportunities for Kids of All Ages

    Science.gov (United States)

    Rogers, L. D.; Valderrama Graff, P.; Bandfield, J. L.; Christensen, P. R.; Klug, S. L.; Deva, B.; Capages, C.

    2007-12-01

    The Mars Public Mapping Project is a web-based education and public outreach tool developed by the Mars Space Flight Facility at Arizona State University. This tool allows the general public to identify and map geologic features on Mars, utilizing Thermal Emission Imaging System (THEMIS) visible images, allowing public participation in authentic scientific research. In addition, participants are able to rate each image (based on a 1 to 5 star scale) to help build a catalog of some of the more appealing and interesting martian surface features. Once participants have identified observable features in an image, they are able to view a map of the global distribution of the many geologic features they just identified. This automatic feedback, through a global distribution map, allows participants to see how their answers compare to the answers of other participants. Participants check boxes "yes, no, or not sure" for each feature that is listed on the Mars Public Mapping Project web page, including surface geologic features such as gullies, sand dunes, dust devil tracks, wind streaks, lava flows, several types of craters, and layers. Each type of feature has a quick and easily accessible description and example image. When a participant moves their mouse over each example thumbnail image, a window pops up with a picture and a description of the feature. This provides a form of "on the job training" for the participants that can vary with their background level. For users who are more comfortable with Mars geology, there is also an advanced feature identification section accessible by a drop down menu. This includes additional features that may be identified, such as streamlined islands, valley networks, chaotic terrain, yardangs, and dark slope streaks. The Mars Public Mapping Project achieves several goals: 1) It engages the public in a manner that encourages active participation in scientific research and learning about geologic features and processes. 2) It helps to

  5. 19 CFR 210.7 - Service of process and other documents; publication of notices.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 3 2010-04-01 2010-04-01 false Service of process and other documents... § 210.7 Service of process and other documents; publication of notices. (a) Manner of service. (1) The service of process and all documents issued by or on behalf of the Commission or the administrative law...

  6. On the ethical analysis of value issues in public decision-making ...

    African Journals Online (AJOL)

    South African Journal of Philosophy ... What difference can such an ethical analysis make to public decision-making? ... value and ethical questions cannot be settled in a rational manner ... values and ethics are so intertwined with emotions and biases that one cannot take them seriously in ... AJOL African Journals Online.

  7. [Health management as part of leadership in public administration].

    Science.gov (United States)

    Matyssek, A K

    2012-02-01

    Leadership includes two tasks within public administration: leading employees by creating healthy working conditions and being led by others. The basis of leading others in a healthy way means dealing with oneself in a healthy manner; this includes typical healthy behavior (i.e., physical activity, improved nutrition, not smoking), which allows for recovery by a good work-life balance and a sensitive dealing with one's own limits. Given these conditions of healthy self care, managers are able to act as role models and lead their employees in a healthy manner. Leading in a healthy manner means showing appreciation, being interested in the employees ("caring"), integrating them in decision-making, allowing transparency, improving the working atmosphere, and finally reducing stress and increasing resources. The latter includes social support, which decreases the danger of employees burning out. The article presents the concept of a 2-day training that is designed for managers in order to raise their awareness for duties related to health management in public administration.

  8. The four spaces of the public library

    DEFF Research Database (Denmark)

    Jochumsen, Henrik; Skot-Hansen, Dorte; Rasmussen, Casper Hvenegaard

    2016-01-01

    During the last 10–15 years the societal context and thereby the library's societal legitimacy has changed in a crucial manner. The development of the Internet and tendencies toward globalisation, detraditionalisation and cultural liberation has challenged the library. Therefore the role...... of the public library in the beginning of the new millennium and as a more concrete tool for designing, developing and redesigning the library....... of the physical library has changed from a more or less passive collection of books and other media to an active space for experience and inspiration and a local meeting point. One could speak of a transformation from ‘collection to connection’ or even from ‘collection to creation’ rather than the predicted...

  9. Public key infrastructure for DOE security research

    Energy Technology Data Exchange (ETDEWEB)

    Aiken, R.; Foster, I.; Johnston, W.E. [and others

    1997-06-01

    This document summarizes the Department of Energy`s Second Joint Energy Research/Defence Programs Security Research Workshop. The workshop, built on the results of the first Joint Workshop which reviewed security requirements represented in a range of mission-critical ER and DP applications, discussed commonalties and differences in ER/DP requirements and approaches, and identified an integrated common set of security research priorities. One significant conclusion of the first workshop was that progress in a broad spectrum of DOE-relevant security problems and applications could best be addressed through public-key cryptography based systems, and therefore depended upon the existence of a robust, broadly deployed public-key infrastructure. Hence, public-key infrastructure ({open_quotes}PKI{close_quotes}) was adopted as a primary focus for the second workshop. The Second Joint Workshop covered a range of DOE security research and deployment efforts, as well as summaries of the state of the art in various areas relating to public-key technologies. Key findings were that a broad range of DOE applications can benefit from security architectures and technologies built on a robust, flexible, widely deployed public-key infrastructure; that there exists a collection of specific requirements for missing or undeveloped PKI functionality, together with a preliminary assessment of how these requirements can be met; that, while commercial developments can be expected to provide many relevant security technologies, there are important capabilities that commercial developments will not address, due to the unique scale, performance, diversity, distributed nature, and sensitivity of DOE applications; that DOE should encourage and support research activities intended to increase understanding of security technology requirements, and to develop critical components not forthcoming from other sources in a timely manner.

  10. 48 CFR 3427.470 - Publication and publicity clause.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 7 2010-10-01 2010-10-01 false Publication and publicity... Copyrights 3427.470 Publication and publicity clause. The contracting officer shall insert the clause in 3452.227-70, Publication and Publicity, in all solicitations and contracts other than purchase orders. ...

  11. Public education - Whose responsibility?

    International Nuclear Information System (INIS)

    Elliott, Alva E.

    1977-01-01

    One of the major problems of the nuclear industry is the lack of public education as to the true facts of nuclear energy. This paper describes some of the misconceptions the general public have concerning reactors, and what program the University of Missouri-Rolla Reactor Facility is following to educate the more than three thousand visitors that tour the facility each year. (author)

  12. Public affairs plan

    Energy Technology Data Exchange (ETDEWEB)

    1994-09-01

    The purpose of the Uranium Mill Tailings Remedial Action (UMTRA) Project Public Affairs Plan is to establish goals for the Fiscal Year 1995 UMTRA public affairs program and identify specific activities to be conducted during the year. It also describes the roles of various agencies involved in the conduct of the public affairs program and defines the functions of the Technical Assistance Contractor (TAC) Public Affairs Department. It integrates and replaces the Public Participation Plan (DOE/AL/62350-47D) and Public Information Plan (DOE/AL/623590-71). The plan describes the US Department of Energy`s (DOE) plans to keep stakeholders and other members of the public informed about project policies, plans, and activities, and provide opportunities for stakeholders and interested segments of the public to participate in project decision-making processes. The plan applies to the UMTRA Project Office; the DOE Albuquerque Operations Office, Office of Intergovernmental and External Affairs (OIEA); the UMTRA TAC; the UMTRA Remedial Action Contractor (RAC); and other cooperating agencies.

  13. EIA Publications Directory 1993

    International Nuclear Information System (INIS)

    1994-01-01

    This directory contains abstracts and ordering information for EIA publications released in the above time period. The abstracts are arranged by broad subject category such as coal, petroleum, natural gas, and electric power. A comprehensive subject index, a title index, and a report number index are included. Each entry gives the title, report number, publication frequency, date, number of pages, and ordering information

  14. Essays on public economics

    NARCIS (Netherlands)

    Jahan Dideh, Mahsa

    2016-01-01

    This dissertation studies a range of topics in public economics. The first two chapters address the optimal provision of productive public goods in two different settings. Using a theoretical model, the first study examines the impact of trade liberalization on the optimal provision of productive

  15. List of publications 1990

    International Nuclear Information System (INIS)

    1991-06-01

    AECL Research is engaged in research and development related to the peaceful applications of nuclear energy. Specifically, the company's mission is to perform the research, development, demonstration and marketing required to apply nuclear sciences and their related technologies for the maximum benefit of Canada. Among our most important products are scientific reports, publications and conference presentations. This document lists our publications for 1990

  16. Public relations and ethics

    Directory of Open Access Journals (Sweden)

    Bajić Milan

    2010-01-01

    Full Text Available The aim of this paper is the analysis of different forms of PR implementation and research its relation with ethics in practice. 'Public relations' is the every-day term that represents the job that is widely used in all aspects of life and work in today's society. Public relations represent a specific form of communication that has a particular application in society. Public relations involve focusing on a public aspect of organization with the aim of building a positive attitude and image. Image of public relations as a profession is often unfairly negative, and the reason for this is unprofessional and unethical relation of individuals towards their profession. In practice ethics in public relations is often considered to be an oxymoron. Ethical thinking of experts in public relations goes from Biblical attitude 'all you want people to do to you, do even so to them' (Matthew 7:12to professional loyalty to organization they work for. Because of unethical appearance it is important to set the rules, in the form of codex by which the public relations professionals will set their behaviors. .

  17. Russia - Public Expenditure Review

    OpenAIRE

    World Bank

    2011-01-01

    The primary objective of the Public Expenditure Review (PER) is to assist the Ministry of Finance (MOF) in identifying opportunities for efficiency gains in some key categories of government expenditure. In this context, policy makers face two related fiscal dilemmas. First, how can expenditure efficiency are increased to provide public services with fewer resources? Second, how can the fi...

  18. Public Management and Evaluation

    NARCIS (Netherlands)

    F.B.L. van der Meer (Frans-Bauke)

    2007-01-01

    textabstractIn public management we can observe a continuous search for its own improvement. New arrangements and procedures, often derived from NPM ideas, but also from other PA insights, are put in place with the expectations that public administration as well as its related agencies and networks

  19. Postmodern Public Administration

    DEFF Research Database (Denmark)

    Bogason, Peter

    2005-01-01

    Discussion of the trends towards more uses of postmodern analysis within the discipline of public administration, particularly in the USA......Discussion of the trends towards more uses of postmodern analysis within the discipline of public administration, particularly in the USA...

  20. Metallurgy Department publications 1988

    International Nuclear Information System (INIS)

    Schroeder Pedersen, A.; Bilde-Soerensen, J.B.

    1989-08-01

    A presentation (including abstracts) of scientific and technical publications and lectures by the staff of the Metallurgy Department during 1988 is given. The list comprises journal papers, conference papers, reports, lectures and poster presentations in the following catagories: Publications, Lectures and Poster Presentations. (author)

  1. Metallurgy Department publications 1989

    International Nuclear Information System (INIS)

    Horsewell, A.

    1990-08-01

    All publiclations by the staff of the Metallurgy Department during 1989 are listed. This list is divided into three sections as follows: Publications (journal and conference papers, reports); Lectures (public lecture presentations) and Posters (poster presentations at conferences and symposia). Abstracts are included. (author)

  2. Public affairs plan

    International Nuclear Information System (INIS)

    1995-09-01

    The purpose of the Uranium Mill Tailings Remedial Action (UMTRA) Project Public Affairs Plan is to establish goals for the fiscal year (FY) 1996 UMTRA Project public affairs program and to identify specific activities to be conducted during the year. It describes the roles of various agencies involved in the public affairs program and defines the functions of the UMTRA Project Technical Assistance Contractor (TAC) Public Affairs Department. It replaces the FY 1995 Public Affairs Plan (DOE/AL/62350-154). The plan also describes the US Department of Energy's (DOE) plans to keep stakeholders and other members of the public informed about UMTRA Project policies, plans, and activities, and provide opportunities for stakeholders and interested segments of the public to participate in UMTRA Project decision-making processes. The plan applies to the UMTRA Project Team; the DOE Grand Junction Projects Office (GJPO); the DOE Albuquerque Operations Office, Office of Public Affairs (OPA); the TAC; the UMTRA Project Remedial Action Contractor (RAC); and other cooperating agencies

  3. Public affairs plan

    International Nuclear Information System (INIS)

    1994-09-01

    The purpose of the Uranium Mill Tailings Remedial Action (UMTRA) Project Public Affairs Plan is to establish goals for the Fiscal Year 1995 UMTRA public affairs program and identify specific activities to be conducted during the year. It also describes the roles of various agencies involved in the conduct of the public affairs program and defines the functions of the Technical Assistance Contractor (TAC) Public Affairs Department. It integrates and replaces the Public Participation Plan (DOE/AL/62350-47D) and Public Information Plan (DOE/AL/623590-71). The plan describes the US Department of Energy's (DOE) plans to keep stakeholders and other members of the public informed about project policies, plans, and activities, and provide opportunities for stakeholders and interested segments of the public to participate in project decision-making processes. The plan applies to the UMTRA Project Office; the DOE Albuquerque Operations Office, Office of Intergovernmental and External Affairs (OIEA); the UMTRA TAC; the UMTRA Remedial Action Contractor (RAC); and other cooperating agencies

  4. Public Sector Expatriate Managers:

    DEFF Research Database (Denmark)

    Fenner, Charles, R., Jr.; Selmer, Jan

    2008-01-01

    Although public sector expatriates are becoming increasingly common, research on them is very limited. There is reason to believe that the situation for expatriates from the public sector may be different than for those from the private sector. This study investigated U.S. Department of Defense a...

  5. Functions of public relations

    OpenAIRE

    Baranov G. V.

    2016-01-01

    the article reveals the importance of communication with the public in the implementation of human rights and the ideals of mankind; characterized by the specificity of public relations in the information culture of belief; PR functions are explained on the criterion of optimization of activity of social interactions on the basis of cultural ideals.

  6. Public Accountancy Handbook.

    Science.gov (United States)

    New York State Education Dept., Albany. Office of the Professions.

    A reference guide to laws, rules, and regulations that govern public accountancy practice in New York State is presented. In addition to identifying licensing requirements/procedures for certified public accountants, general provisions of Title VIII of the Education Law are covered, along with state management, professional misconduct, and…

  7. Orkestre er public service

    DEFF Research Database (Denmark)

    Fjeldsøe, Michael

    2017-01-01

    kommentar til debatten om danske symfoniorkestre - kritik af Informations manglende forståelse af europæiske radiofoniers orkestres rolle som public service......kommentar til debatten om danske symfoniorkestre - kritik af Informations manglende forståelse af europæiske radiofoniers orkestres rolle som public service...

  8. Public Communication Strategy Development: Focused on the Public Opinion Poll and Lessons Learned from the Survey on Nuclear Safety

    International Nuclear Information System (INIS)

    Hah, Yeon Hee; Chung, Yun Hyung; Lee, Gey Hwi; Choi, Yong Seog

    2006-01-01

    The fundamental objective of nuclear regulatory organization is to ensure that nuclear activities are conducted at all times in an acceptably safe manner. When we are talking about 'acceptable' safety, it means that we have to try to achieve the safety level as 'acceptable' as perceived by the public, not the one conceptualized by the technology itself. In meeting this objective, the regulatory organization continues to ensure that its activities are transparent, technically sound, credible, and independent. More importantly, it should strive to ensure that the public are well informed about such activities and are confident upon regulatory organization. Public communication is one of the key approaches to attain such a goal. The availability of on-line communications based on the development of information technology has made a major impact on the public's better awareness of nuclear safety issues. Nuclear regulatory organization and operators are increasingly under pressure to communicate more actively with the public to satisfy their demand for knowing about the safety of nuclear facility. In coping with such challenges, KINS has developed a public information and communication policy to make public confident in their information and thus to earn the public trust and confidence toward nuclear safety. It is widely acknowledged that public communication for ensuring nuclear safety has been an integral part of the operators' activity. However, the roles and activities by the regulator in the area of public communication are increasingly required as the public become more interested in and concerned about safety issues. Taking this into account, KINS has carried out several activities to be well-informed about how the public perceive nuclear safety and how much they are satisfied with the regulator's activities, etc

  9. Public education and participation

    International Nuclear Information System (INIS)

    Kelly, J.E.

    1982-01-01

    As prescribed in Step 1 of the Public Education and Participation Process (attachment 1), industry, public interest groups, and decision-makers were briefed about the Subseabed Disposal Program. In regard to public interest groups, Drs. Hollister and Kelly were invited to present the technical and policy aspects of the Subseabed Program at a public forum in Hawaii sponsored by the Hawaii League of Women Voters, the Health Physics Society, and the East-West Center. The sponsors videotaped the forum for a film, entitled Slowly Dying Embers: Radioactive Waste and the Pacific, which will be shown on television in Hawaii. In response to requests for information about the Subseabed Program, Congressional Staff, Representatives, and Senators (attachment 2) were briefed about the Subseabed Program as legislation related to the Program moved through Congress (attachment 3). Science oriented publications also were contacted about the Program

  10. Leading Public Design

    DEFF Research Database (Denmark)

    Bason, Christian

    at all levels have taken up a variety of approaches inspired by the field of design, often in response to growing pressures to innovate. But how these approaches influence public innovation – how they change the roles of public managers, how they help managers generate new ideas and solutions...... – and whether, as some have suggested, they might signal the rise of new governance models or paradigms – these issues have not, with perhaps a few exceptions, been rigorously explored. In this thesis, I explore these issues by examining the experiences of public managers who have pioneered the use of design...... approaches. More specifically, I confront three questions: • Characterizing design practice: Within public sector organizations, what does the application of design approaches entail? Why do public managers look to and commission design, and what tools, techniques, processes and methods are brought into play...

  11. DOE headquarters publications

    International Nuclear Information System (INIS)

    1978-09-01

    This bibliography provides listings of (mainly policy and programmatic) publications issued from the U.S. Department of Energy, Washington, D.C. The listings are arranged by the ''report code'' assigned to each of the major organizations at DOE Headquarters, followed by the three categories of environmental reports issued from DOE Headquarters. All of the publications listed, except for those shown as still ''in preparation,'' may be seen in the Energy Library. A title index arranged by title keywords follows the listings. Certain publications are omitted. They include such items as pamphlets, ''fact sheets,'' bulletins and weekly/monthly issuances of DOE's Energy Information Administration and Economic Regulatory Administration, and employee bulletins and newsletters. Omitted from the bibliography altogether are headquarters publications assigned other types of report codes--e.g., ''HCP'' (Headquarters Contractor Publication) and ''CONF'' (conference proceedings)

  12. NRCN publications in 1977

    International Nuclear Information System (INIS)

    Kuznietz, M.; Makeveth, D.; Israeli, O.

    1978-09-01

    The front-pages of 24 works, written by employees of the Nuclear Research Center - Negev, and published in 1977 by the Publishing Department of its Scientific and Technical Information Service, as publications in the NRCN series or in the NRCN(ER), NRCN(GP), and NRCN(TN) sub-series, are presented. The front-pages include the respective NRCN serial or sub-serial number, title, authors, date of publication, and abstract (and date of receipt, if substantially preceding the date of publication) in the original language (Hebrew or English). The front-page translations (into English or Hebrew, respectively) are also included. Various data, concerning the NRCN publications in 1977, are given, as well as a list of all publications and alphabetical lists of authors (in Hebrew and Latin transliteration)

  13. Publication of the bulletin

    CERN Document Server

    DSU Unit

    2008-01-01

    The final edition (Nos 51-52/2008 and 1-2/2009) of the last Weekly Bulletin of the year will be published on Friday 12 December and will cover events at CERN from 15 December 2008 to 12 January 2009. Announcements for publication in this issue should reach the Publication Section (Communication group) or the Staff Association, as appropriate by noon, on Tuesday 9 December. Bulletin publication 2009 The table below lists the 2009 publication dates for the paper version of the Bulletin and the corresponding deadlines for the submission of announcements. Please note that all announcements must be submitted by 12.00 midday on Tuesdays at the latest. Bulletin No. Week number Submission of announcements (before 12.00 midday) Publication date 3-4 Tuesday 6 January Monday 12 January 5-6 Tuesday 20 January Monday 26 January 7-8 Tuesday 3 February Monday 9 February 9-10 Tuesday 17 Februar...

  14. AtPP2CG1, a protein phosphatase 2C, positively regulates salt tolerance of Arabidopsis in abscisic acid-dependent manner

    International Nuclear Information System (INIS)

    Liu, Xin; Zhu, Yanming; Zhai, Hong; Cai, Hua; Ji, Wei; Luo, Xiao; Li, Jing; Bai, Xi

    2012-01-01

    Highlights: ► AtPP2CG1 positively regulates salt tolerance in ABA-dependent manner. ► AtPP2CG1 up-regulates the expression of marker genes in different pathways. ► AtPP2CG1 expresses in the vascular system and trichomes of Arabidopsis. -- Abstract: AtPP2CG1 (Arabidopsis thaliana protein phosphatase 2C G Group 1) was predicted as an abiotic stress candidate gene by bioinformatic analysis in our previous study. The gene encodes a putative protein phosphatase 2C that belongs to Group G of PP2C. There is no report of Group G genes involved in abiotic stress so far. Real-time RT-PCR analysis showed that AtPP2CG1 expression was induced by salt, drought, and abscisic acid (ABA) treatment. The expression levels of AtPP2CG1 in the ABA synthesis-deficient mutant abi2–3 were much lower than that in WT plants under salt stress suggesting that the expression of AtPP2CG1 acts in an ABA-dependent manner. Over-expression of AtPP2CG1 led to enhanced salt tolerance, whereas its loss of function caused decreased salt tolerance. These results indicate that AtPP2CG1 positively regulates salt stress in an ABA-dependent manner. Under salt treatment, AtPP2CG1 up-regulated the expression levels of stress-responsive genes, including RD29A, RD29B, DREB2A and KIN1. GUS activity was detected in roots, leaves, stems, flower, and trichomes of AtPP2CG1 promoter–GUS transgenic plants. AtPP2CG1 protein was localized in nucleus and cytoplasm via AtPP2CG1:eGFP and YFP:AtPP2CG1 fusion approaches.

  15. Ex ante and ex post control of the public interest in public-private partnership agreements

    Directory of Open Access Journals (Sweden)

    Ćirić Aleksandar

    2016-01-01

    Full Text Available The paper analyzes the provisions of public-private partnership agreement (hereinafter: the 'PPP' in the context of control of the public interest in the process of preparing and implementing PPP projects. PPP agreements are a mechanism for defining rights and obligations of public and private partners in the process of realization of a particular PPP project. In spite of being defined as partnership, public and private interests largely remain competitive. However, in case it is not possible to achieve both interests at the same time, the public interest is deemed to have priority. The proper implementation of the supremacy of public interests over the private ones calls for an appropriate contractual definition of conditions and manner for such implementation. The methodology for exercising control of the public interest in PPP projects through agreements on public-private partnership has two aspects. The first aspect is to provide verifiable parameters of legal, economic and technological conditions, as well as the procedure for selecting the best bid for the realization of public-private partnership (ex ante methodological aspect; these conditions are the basis for future PPP agreement. Ex ante methodology has to provide instruments for control of the public interest through pre-established mechanisms aimed at verifying whether the private partner has fulfilled the agreement. If such verification is not possible or if it is considered unreasonable (either financially or otherwise, it may raise an issue whether the public-private partnership is an adequate modality for implementation of the project aimed at promoting the public interest; it further implies that the public partner should resort to other options (employing 'internal' resources or taking a loan for financing the projects of public interests. The second aspect is that the PPP agreements are to provide for the proper implementation of the elements that ensure some flexibility in

  16. Topical issues of transparency in implementing public control in municipalentities

    Directory of Open Access Journals (Sweden)

    Denis Stepanovich Mikheyev

    2015-06-01

    Full Text Available Objective to investigate the mechanisms of citizens and public institutions participation in public control in the municipalities. According to the author the monitoring process should involve the greatest possible range of subjects and the controlling measures are to be taken in a transparent and open manner. Therefore forms of citizen participation in public control should be investigated from the standpoint of the principle of openness of local government. Methods the universal dialectic method which was applied to the analysis of norms of the Federal law quotOn fundamentals of public control in the Russian Federationquot which is the legal base for the implementation of the institution of public control. When studying the problem of adequate legal regulation of the control institution the formal legal method was also used. Other methods were used for evaluation of the acquired knowledge in particular comparative legal and systemicstructural methods. Results on the basis of legal norms analysis based on the legal nature of the local government institution as the level of public authorities which is closest to the citizens the conclusion was made about the lack of legal regulation relating to the public control subjects in municipalities. The numerous public organizations operating in local communities were not fixed by legislative norms as subjects of public control. Scientific novelty the author has grounded the proposals for amending the abovementioned Federal law the legislation of Federation subjects and the municipal regulations by adding a number of new subjects of public control inherent to the municipal level. Practical value the conclusions and suggestions formulated in the study will contribute to the active implementation of public control in the municipalities will enhance the ability of citizens and civil society institutions to monitor the authorities and will have a positive impact on transparency of local selfgovernment. The

  17. Public safety around dams

    Energy Technology Data Exchange (ETDEWEB)

    Bourassa, H [Centre d' expertise hydrique du Quebec, Quebec, PQ (Canada)

    2009-07-01

    Fourty public dams are managed on a real-time basis by the Centre d'expertise hydrique du Quebec (CEHQ). This presentation described the public dams owned by the CEHQ and discussed the public safety measures at the dams. The dams serve various purposes, including protection against floods; industrial or drinking water supply; resort or recreational activities; hydroelectric development; and wildlife conservation. Trigger events were also discussed, such as the complaint at Rapides-des-Cedres dam and deaths that occurred in 2004 when water from a dam was released without warning. Several photographs were presented to illustrate that people were unaware of the danger. Initiatives aimed at raising awareness and studying public safety issues were discussed. A pilot project was launched and a permanent committee was created to evaluate all aspects of public safety at the dams owned by CEHQ. The first tasks of the committee were to establish requirements for waterway safety barriers, both upstream and downstream, for all public dams; to establish requirements for safety signage for all public dams; and to develop criteria to decide on safety signage at each dam. figs.

  18. Public safety around dams

    Energy Technology Data Exchange (ETDEWEB)

    Bourassa, H. [Centre d' expertise hydrique du Quebec, Quebec, PQ (Canada)

    2009-07-01

    Fourty public dams are managed on a real-time basis by the Centre d'expertise hydrique du Quebec (CEHQ). This presentation described the public dams owned by the CEHQ and discussed the public safety measures at the dams. The dams serve various purposes, including protection against floods; industrial or drinking water supply; resort or recreational activities; hydroelectric development; and wildlife conservation. Trigger events were also discussed, such as the complaint at Rapides-des-Cedres dam and deaths that occurred in 2004 when water from a dam was released without warning. Several photographs were presented to illustrate that people were unaware of the danger. Initiatives aimed at raising awareness and studying public safety issues were discussed. A pilot project was launched and a permanent committee was created to evaluate all aspects of public safety at the dams owned by CEHQ. The first tasks of the committee were to establish requirements for waterway safety barriers, both upstream and downstream, for all public dams; to establish requirements for safety signage for all public dams; and to develop criteria to decide on safety signage at each dam. figs.

  19. Public interest group involvement

    International Nuclear Information System (INIS)

    Shelley, P.

    1986-01-01

    Including public interest groups in the siting process for nuclear waste disposal facilities is of great importance. Controversial sitings often result in litigation, but involving public interest groups early in the process will lessen the change of this. They act as surrogates for the general public and should be considered as members of the team. It is important to remember though, that all public interest groups are different. In choosing public panels such as public advisory committees, members should not be chosen on the basis of some quota. Opposition groups should not be excluded. Also, it is important to put the right person in charge of the committee. The goal of public involvement is to identify the conflicts. This must be done during the decision process, because conflicts must be known before they can be eliminated. Regarding litigation, it is important to ease through and around legal battles. If the siting process has integrity and a good faith effort has been shown, the court should uphold the effort. In addition, it is important to be negotiable and to eliminate shortcuts

  20. Publication of the bulletin

    CERN Multimedia

    2007-01-01

    The final edition (Nos 51-52/2007 and 1-2-3/2008) of the last Bulletin of the year will appear on Friday 14 December and will cover events at CERN from 17 December 2007 to 18 January 2008. Announcements for publication in this issue should reach the Publication Section (Communication group) or the Staff Association, as appropriate, by noon on Tuesday 11 December. The table below lists the 2008 publication dates for the paper version of the Bulletin and the corresponding deadlines for the submission of announcements. Please note that all announcements must be submitted by 12.00 midday on Tuesdays at the latest. Bulletin publication 2008 Bulletin N° 4-5 Publication: Monday 21 january Submission deadline for announcements: Tuesday 15 January Bulletin N° 6-7 Publication: Monday 4 february Submission deadline for announcements: Tuesday 29 January Bulletin N° 8-9 Publication: Monday 18 february Submission deadline for announcements: Tuesday 12 February Bulletin N° 10-11 P...

  1. The Mandate System for the Belgian Public Prosecution

    Directory of Open Access Journals (Sweden)

    Bruno BROUCKER

    2009-12-01

    Full Text Available The law of 22 December 1998 introduced the mandate system for the heads of the Public Prosecution offices, which were appointed permanent before that. Theoretically, such a system needs to enhance, within the organization, effectiveness, efficiency, responsabilisation, and goal-orientation. However, the mandate system within the Belgian Public Prosecution was introduced prematurely, for dubious reasons and in a precipitate manner. In the current situation, the position of the mandate holder is uncertain, with a bounded autonomy and a low wage increase. Moreover, it remains impossible to intervene in the policy of appointed heads of office (during their mandate, the efficiency and effectiveness is only increased in some prosecution offices and a contract containing actual management responsibilities is absent. In sum: there is a large gap between the theoretical principles of mandate systems and the way it is introduced in the Belgian Public Prosecution.

  2. How to build public information and understanding : some French examples

    International Nuclear Information System (INIS)

    Bergeonneau, P.

    1998-01-01

    Public perception of nuclear issues and of the environmental impact is largely dependent on the general public's knowledge and understanding of complex scientific and technical matters which characterize the nuclear world, and on information provided by mass media which primarily tends to emphasize dramatic aspects. Consequently public information must be provided as completely and in as straightforward a manner as possible, and be adapted for people who do not have a clear understanding of nuclear technologies. Information must be objective in all cases, in order to increase credibility over time. This is important because confidence in scientists, and more generally in technical progress, has been eroded. In addition, when providing information, one has to anticipate events as much as possible in order to avoid misunderstandings when crises occur. Explanations during crises will then be better understood and confidence will be maintained. Examples of campaigns undertaken by the French Atomic Energy Commission are given in this paper. (author)

  3. Public regulators and CSR

    DEFF Research Database (Denmark)

    Buhmann, Karin

    2016-01-01

    of such public regulatory governance, this article explores and explains developments towards a juridification of CSR entailing efforts by public regulators to reach beyond jurisdictional and territorial limitations of conventional public law to address adverse effects of transnational economic activity. Through...... analysis of an expansion of law into the normative framing of what constitutes responsible business conduct, we demonstrate a process of juridification entailing a legal framing of social expectations of companies, a proliferation of law into the field of business ethics, and an increased regulation by law...

  4. Conflicts in Public Procurement

    Directory of Open Access Journals (Sweden)

    Helena Lindskog

    2013-01-01

    Full Text Available During the last couple of years, there has been a tendency to include more and more political goals into public procurements, such as environmental and societal considerations. This can result in higher prices paid by the public sector compared with similar procurements in the private sector. The decision makers at local level are elected and should represent the interest of their communities and voters, which includes promoting regional/local companies and economic development. This task can sometimes get into conflict with public procurement law or the political goals of a central government. (original abstract

  5. 'The Public Spectre'

    DEFF Research Database (Denmark)

    Jepsen, Kim Sune Karrasch; Bertilsson, Margareta; Delgado, Ana

    2016-01-01

    This paper is concerned with how forms of publics come into being in situations of technological innovation and change. The paper attends to sudden social formations which arise as responses to what is perceived of as threatening socio-technical inventions into the routine of everyday social life....... We revisit the Dewey-Lippmann debate, and we look at spontaneous citizens’ reactions towards emerging technologies such as Google Street View as well as we discuss some institutionally organized events of public engagement with science. As we explore the formation of publics inside and outside...

  6. Public Engagement with Science

    DEFF Research Database (Denmark)

    Irwin, Alan

    2014-01-01

    ). The ‘deficit theory’ which still today characterize many scientific activities that address citizen can be criticized for ‘one-way communication’, ‘sanctity of expertise’, and treatment of the publics as ‘homogeneous’. When arguing for the need for public engagement with science it is question about...... for dialogue exercises look microscopic against the backdrop of global science and its governance. Maybe it has been over-promised what such public engagement exercises can deliver. We can safely conclude that, despite all the ‘from deficit to democracy’ talk, no such easy shift has been made. At best, partial...

  7. LTE for public safety

    CERN Document Server

    Liebhart, Rainer; Wong, Curt; Merkel , Jürgen

    2015-01-01

    The aim of the book is to educate government agencies, operators, vendors and other regulatory institutions how LTE can be deployed to serve public safety market and offer regulatory / public safety features. It is written in such a way that it can be understood by both technical and non-technical personnel with just introductory knowledge in wireless communication. Some sections and chapters about public safety services offered by LTE network are intended to be understood by anyone with no knowledge in wireless communication.

  8. Inflammatory Human Umbilical Cord-Derived Mesenchymal Stem Cells Promote Stem Cell-Like Characteristics of Cancer Cells in an IL-1β-Dependent Manner

    Directory of Open Access Journals (Sweden)

    Xiaohe Luo

    2018-01-01

    Full Text Available To ensure the safety of clinical applications of MSCs, thorough understanding of their impacts on tumor initiation and progression is essential. Here, to further explore the complex dialog between MSCs and tumor cells, umbilical cord-derived mesenchymal stem cells (UC-MSCs were employed to be cocultured with either breast or ovarian cancer cells. Though having no obvious influence on proliferation or apoptosis, UC-MSCs exerted intense stem cell-like properties promoting effects on both cancer models. Cocultured cancer cells showed enriched side population, enhanced sphere formation ability, and upregulated pluripotency-associated stem cell markers. Human cytokine array and real-time PCR revealed a panel of MSC-derived prostemness cytokines CCL2, CXCL1, IL-8, and IL-6 which were induced upon coculturing. We further revealed IL-1β, a well-characterized proinflammatory cytokine, to be the inducer of these prostemness cytokines, which was generated from inflammatory UC-MSCs in an autocrine manner. Additionally, with introduction of IL-1RA (an IL-1 receptor antagonist into the coculturing system, the stem cell-like characteristics promoting effects of inflammatory UC-MSCs were partially blocked. Taken together, these findings suggest that transduced inflammatory MSCs work as a major source of IL-1β in tumor microenvironment and initiate the formation of prostemness niche via regulating their secretome in an IL-1β-dependent manner.

  9. dSir2 in the Adult Fat Body, but Not in Muscles, Regulates Life Span in a Diet-Dependent Manner

    Directory of Open Access Journals (Sweden)

    Kushal Kr. Banerjee

    2012-12-01

    Full Text Available Sir2, an evolutionarily conserved NAD+-dependent deacetylase, has been implicated as a key factor in mediating organismal life span. However, recent contradictory findings have brought into question the role of Sir2 and its orthologs in regulating organismal longevity. In this study, we report that Drosophila Sir2 (dSir2 in the adult fat body regulates longevity in a diet-dependent manner. We used inducible Gal4 drivers to knock down and overexpress dSir2 in a tissue-specific manner. A diet-dependent life span phenotype of dSir2 perturbations (both knockdown and overexpression in the fat body, but not muscles, negates the effects of background genetic mutations. In addition to providing clarity to the field, our study contrasts the ability of dSir2 in two metabolic tissues to affect longevity. We also show that dSir2 knockdown abrogates fat-body dFOXO-dependent life span extension. This report highlights the importance of the interplay between genetic factors and dietary inputs in determining organismal life spans.

  10. Maternal di-(2-ethylhexyl) phthalate exposure during pregnancy causes fetal growth restriction in a stage-specific but gender-independent manner.

    Science.gov (United States)

    Shen, Ru; Zhao, Ling-Li; Yu, Zhen; Zhang, Cheng; Chen, Yuan-Hua; Wang, Hua; Zhang, Zhi-Hui; Xu, De-Xiang

    2017-01-01

    Di (2-ethylhexyl) phthalate (DEHP) is male developmental toxicant that impairs testis development with reduced anogenital distance. The present study aimed to investigate whether maternal DEHP exposure during pregnancy causes intrauterine growth restriction (IUGR) in a gender-specific manner and to identify the critical window of DEHP-induced fetal IUGR. Pregnant mice were administered with DEHP (0, 50 or 200mg/kg) by gavage. Fetal IUGR was observed not only in males but also in females when litters were exposed to DEHP on gestational day (GD)0-GD17. Interestingly, fetal weight and crown-rump length were reduced, markedly in dams with DEHP on GD13-GD17, slightly in dams with on GD7-GD12, but not in dams with on GD0-GD6. Further analysis showed that maternal DEHP exposure on GD7-GD12 inhibited cell proliferation, lowered placental weight, and reduced blood sinusoid area in placental labyrinth layer. These results suggest that maternal DEHP exposure induces IUGR in a stage-specific but gender-independent manner. Copyright © 2016 Elsevier Inc. All rights reserved.

  11. Maturation, fertilisation and culture of bovine oocytes and embryos in an individually identifiable manner: a tool for studying oocyte developmental competence.

    Science.gov (United States)

    Matoba, Satoko; Fair, Trudee; Lonergan, Patrick

    2010-01-01

    The ability to successfully culture oocytes and embryos individually would facilitate the study of the relationship between follicle parameters and oocyte developmental competence, in order to identify markers of competent oocytes, as well as the ability to use small numbers of oocytes from an individual donor such as when ovum pick-up is carried out. Using a total of 3118 oocytes, the aim of the present study was to develop a system capable of supporting the development of immature bovine oocytes to the blastocyst stage in an individually identifiable manner. Initially, post-fertilisation embryo culture in the Well-of-the-Well (WOW) system, on the cell adhesive Cell-Tak or in polyester mesh was tested and shown to result in similar development to embryos cultured in standard group culture. The results demonstrate that it is possible to culture bovine oocytes to the blastocyst stage in an individually identifiable manner in all three culture systems with comparable success rates. This permits the localisation and identification of individual embryos throughout preimplantation development in vitro while retaining the developmental benefits of group culture. In terms of ease of preparation and use, culture in isolation within the strands of a polyester mesh is preferable.

  12. ERβ1 inhibits the migration and invasion of breast cancer cells through upregulation of E-cadherin in a Id1-dependent manner

    International Nuclear Information System (INIS)

    Zhou, Yan; Ming, Jia; Xu, Yan; Zhang, Yi; Jiang, Jun

    2015-01-01

    Highlights: • Expression of ERβ1 was positively correlated with E-cadherin in breast cancer cell. • ERβ1 upregulates E-cadherin expression in breast cancer cell lines. • ERβ1 upregulates E-cadherin expression in a Id1-dependent manner. - Abstract: ERβ1 is a member of the nuclear receptor superfamily of ligand-regulated transcription factors. It plays an important role in regulating the progression of breast cancer. However, the mechanisms of ERβ1 in tumorigenesis, metastasis and prognosis are still not fully clear. In this study, we showed that the expression of ERβ1 was positively correlated with E-cadherin expression in breast cancer cell lines. In addition, we found that ERβ1 upregulates E-cadherin expression in breast cancer cell lines. Furthermore, we also found that ERβ1 inhibits the migration and invasion of breast cancer cells and upregulated E-cadherin expression in a Id1-dependent manner. Taken together, our study provides further understanding of the molecular mechanism of ERβ1 in tumor metastasis and suggests the feasibility of developing novel therapeutic approaches to target Id1 to inhibit breast cancer metastasis

  13. PRMT1 and PRMT4 Regulate Oxidative Stress-Induced Retinal Pigment Epithelial Cell Damage in SIRT1-Dependent and SIRT1-Independent Manners

    Directory of Open Access Journals (Sweden)

    Dong-Il Kim

    2015-01-01

    Full Text Available Oxidative stress-induced retinal pigment epithelial (RPE cell damage is involved in the progression of diabetic retinopathy. Arginine methylation catalyzed by protein arginine methyltransferases (PRMTs has emerged as an important histone modification involved in diverse diseases. Sirtuin (SIRT1 is a protein deacetylase implicated in the onset of metabolic diseases. Therefore, we examined the roles of type I PRMTs and their relationship with SIRT1 in human RPE cells under H2O2-induced oxidative stress. H2O2 treatment increased PRMT1 and PRMT4 expression but decreased SIRT1 expression. Similar to H2O2 treatment, PRMT1 or PRMT4 overexpression increased RPE cell damage. Moreover, the H2O2-induced RPE cell damage was attenuated by PRMT1 or PRMT4 knockdown and SIRT1 overexpression. In this study, we revealed that SIRT1 expression was regulated by PRMT1 but not by PRMT4. Finally, we found that PRMT1 and PRMT4 expression is increased in the RPE layer of streptozotocin-treated rats. Taken together, we demonstrated that oxidative stress induces apoptosis both via PRMT1 in a SIRT1-dependent manner and via PRMT4 in a SIRT1-independent manner. The inhibition of the expression of type I PRMTs, especially PRMT1 and PRMT4, and increased SIRT1 could be therapeutic approaches for diabetic retinopathy.

  14. Planning Public Transport

    DEFF Research Database (Denmark)

    Andersen, Jonas Lohmann Elkjær; Landex, Alex

    2009-01-01

    This good practice guide is composed for the master course 13120 Public Transport Planning held at the Department of Transport, Technical University of Denmark. It is intended to use as guide in the different planning aspects and assignments of the course. Since the course is about the planning...... of new public transport infrastructure this guide also focuses on the planning of new infrastructure. Furthermore, the new infrastructure in the course is expected to be a light rail and even though this guide aims at being general for public transport some of the issues evidently become more relevant...... will enable a capability for planning both bus and rail. The guide is build as a full sketch investigation of a new public transport project ranging chronological from project clarification to physical and timetable planning to traffic modeling and project appraisal. The same steps that are expected...

  15. MMLEADS Public Use File

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Medicare-Medicaid Linked Enrollee Analytic Data Source (MMLEADS) Public Use File (PUF) contains demographic, enrollment, condition prevalence, utilization, and...

  16. HMI scientific publications 1991

    International Nuclear Information System (INIS)

    Ruehlemann, S.

    1992-08-01

    The publications of the staff of the Hahn-Meitner-Institute (HMI) are listed in this catalogue. It contains articles which have been published in journals, books or book chapters, theses or HMI-reports in 1988. Only those publications are included of which a copy was provided to the library. Each title is indexed by a publication number, composed of a code indicating the field of research, the year of publication, and a running number. The fields of research are physics, chemistry, solid state and materials research, and information techniques. The research reactor BER II, the heavy ion accelerator VICKSI, the central information and computer operation, the electronics, and the central library belong to the infrastructure of the HMI. (orig.) [de

  17. Constituting Public Engagement

    DEFF Research Database (Denmark)

    Davies, Sarah Rachael

    2013-01-01

    understanding of science to those of public engagement with science and technology (PEST), and the histories, or genealogies, of such models. Data from two qualitative studies-a case study of one of the United Kingdom'ssix Beacons for Public Engagement and a study of contract research staff-are used......This article uses data from two U.K. studies in order to explore the meanings attached to public engagement. It focuses on two issues of importance to contemporary discussions of science communication: the degree to which there has been a smooth transition, in practice, from models of public...... to characterize the ways in which U.K. academic communities understand PEST. It is argued that engagement is construed as multiple, relational, and outcomes oriented, with seven key outcomes ranging from better research to empowered individuals. These differences are traced to personal and professional...

  18. NRCN publications in 1976

    International Nuclear Information System (INIS)

    Kuznietz, M.; Wiener, U.; Israeli, O.

    1977-04-01

    The front-pages of 35 works, written by employees of the Nuclear Research Center - Negev, and published in 1976 by the Publishing Department of its Scientific and Technical Information Service, as publications in the NRCN series or in the NRCN(ER), NRCN(ML), and NRCN(TN) sub-series, are presented. The front-pages include the respective NRCN serial or sub-serial number, title, authors, date of publication, and abstract (and date of receipt, if substantially preceding the date of publication) in the original language (Hebrew or English). The front-page translations (into English or Hebrew), if published, are also included. Various data, concerning the NRCN publications in 1976, are given as well as alphabetical lists of authors (in Hebrew and Latin transliteration)

  19. CDBG Public Improvements Activity

    Data.gov (United States)

    Department of Housing and Urban Development — CDBG activity related to public improvements, including senior centers, youth centers, parks, street improvements, water/sewer improvements, child care centers, fire...

  20. Public sector innovation

    DEFF Research Database (Denmark)

    Wegener, Charlotte

    2012-01-01

    This paper explores the value dimension of public innovation in the light of practitioners’ values and asks why there seems to be a clash between innovation imperatives and workplace practices in the public sector. The paper contributes to the research on public innovation from a practice...... perspective by providing evidence from an ethnographic field study on innovation in social and health care studies in Denmark. These studies are part of the vocational education and training (VET) system, which combines coursework at a college and internship in the elder care sector. The study is thus cross...... initiate innovations grounded in their values. The main point put forward is that the value dimension of public innovation must be understood not only in terms of value creation (economic or non-economic), which frontline practitioners are required to contribute to, but also as value-based practices...

  1. Public Service? Tax Credits?

    Science.gov (United States)

    Shanker, Albert

    1982-01-01

    Acknowledges the good work of private schools but resists the provision of further direct or indirect government aid to these schools. Argues that tax credits will adversely affect public education and American society. (Author/WD)

  2. Climatological Data Publication

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Major US airport weather stations monthly and annual publication containing station daily maximum and minimum temperatures, precipitation amounts, and monthly...

  3. Public Land Survey filled

    Data.gov (United States)

    California Natural Resource Agency — The 'PLSFILL' layer is a polygon coverage depicting the township, range and sections contained in the Public Land Survey System grid for the State of California....

  4. Public Insurance and Equality

    DEFF Research Database (Denmark)

    Landes, Xavier; Néron, Pierre-Yves

    2015-01-01

    Heath (among other political theorists) considers that the principle of efficiency provides a better normative explanation and justification of public insurance than the egalitarian account. According to this view, the fact that the state is involved in the provision of specific insurance (primarily......Public insurance is commonly assimilated with redistributive tools mobilized by the welfare state in the pursuit of an egalitarian ideal. This view contains some truth, since the result of insurance, at a given moment, is the redistribution of resources from the lucky to unlucky. However, Joseph...... surrounding public insurance as a redistributive tool, advancing the idea that public insurance may be a relational egalitarian tool. It then presents a number of relational arguments in favor of the involvement of the state in the provision of specific forms of insurance, arguments that have been overlooked...

  5. Public employees leadership institute.

    Science.gov (United States)

    2011-08-01

    Public agencies in Iowa are continually challenged with reduced staff levels, reduced budgets, and increased expectations for services provided. Responding to these demands requires a well-informed and coordinated team that includes professionals, su...

  6. Children's Health Publications

    Science.gov (United States)

    Each title has a brief description and link for downloading the full text. Includes the publications catalog, the Child Health Champion resource guide, student curriculum materials, reports, fact sheets, and booklets/brochures of advice and tools.

  7. Publications about Asthma

    Science.gov (United States)

    EPA provides the general public, partners, media outlets and health care professionals with a wide variety of asthma resources at no-cost. EPA develops resources to share information about asthma, its triggers, and comprehensive asthma management.

  8. Monographs - SEER Publications

    Science.gov (United States)

    In-depth publications on topics in cancer statistics, including collaborative staging and registry data, cancer survival from a policy and clinical perspective, a description of cancer in American Indians/Alaska Natives, and measures of health disparities.

  9. Sustainable Public Bids

    Directory of Open Access Journals (Sweden)

    Gil César Costa de Paula

    2016-06-01

    Full Text Available In this article we will discuss the issue of sustainability in public procurement, given that the government in Brazil is constituted as a great promoter of economic development and needs to adapt its acquisitions worldwide sustainability agenda.

  10. Discriminatory public procurement policies

    NARCIS (Netherlands)

    Ssennoga, Francis

    2010-01-01

    To achieve social economic objectives, governments usually institute discriminatory practices in their country’s public procurement framework. Discriminatory procurement is the practice by governments to favour their own domestic suppliers over foreign firms for advertised contracts. Favouring

  11. Public Key Cryptography.

    Science.gov (United States)

    Tapson, Frank

    1996-01-01

    Describes public key cryptography, also known as RSA, which is a system using two keys, one used to put a message into cipher and another used to decipher the message. Presents examples using small prime numbers. (MKR)

  12. Public Waters Inventory Maps

    Data.gov (United States)

    Minnesota Department of Natural Resources — This theme is a scanned and rectified version of the Minnesota DNR - Division of Waters "Public Waters Inventory" (PWI) maps. DNR Waters utilizes a small scale...

  13. Public Outreach and Participation

    National Research Council Canada - National Science Library

    2007-01-01

    The U.S. Army Chemical Materials Agency (CMA) is responsible for safely storing and eliminating chemical agent and munitions stockpiles while ensuring the safety of the public, the work force, and the environment...

  14. TSA Public Inquiry Data

    Data.gov (United States)

    Department of Homeland Security — All non-media public inquiries and complaints and responses to inquiries received by telephone, e-mail and fax, and handles contacts in English and Spanish. The data...

  15. NAIP Public Image Services

    Data.gov (United States)

    Farm Service Agency, Department of Agriculture — This map provides a preview and information about the National Agriculture Imagery Program (NAIP) image services available on the APFO public image server. Click on...

  16. Publication of the Bulletin

    CERN Multimedia

    The final edition (Nos 51-52/2009 and 1-2/2010) of the last Weekly Bulletin of the year will be published on Friday 11 December and will cover events at CERN from 14 December 2009 to 8 January 2010. Announcements for publication in this issue should reach the Publication Section (Communications group) or the Staff Association, as appropriate, by noon on Tuesday 8 December. Bulletin publication 2010 The table below lists the 2010 publication dates for the paper version of the Bulletin and the corresponding deadlines for the submission of announcements. Please note that all announcements must be submitted by 12.00 midday on Tuesdays at the latest. Bulletin No. Week number Submission of announcements (before 12.00 midday) Bulletin Web version Bulletin Paper version 2-3 Tuesday 5 January Friday 8 and 15 January Wednesday 13 J...

  17. Storm Data Publication

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — 'Storm Data and Unusual Weather Phenomena' is a monthly publication containing a chronological listing, by state, of hurricanes, tornadoes, thunderstorms, hail,...

  18. Child public health

    National Research Council Canada - National Science Library

    Blair, Mitch

    2010-01-01

    "Despite children making up around a quarter of the population, the first edition of this book was the first to focus on a public health approach to the health and sickness of children and young people...

  19. The participatory public library

    DEFF Research Database (Denmark)

    Rasmussen, Casper Hvenegaard

    2016-01-01

    of theoretical approaches and practical examples to obtain a varied understanding of user participation in public libraries. Research fields outside library and information science have developed a wide range of theoretical approaches on user participation. Examples from cultural policy, museum studies......Purpose From collection to connection has been a buzzword in the library world for more than a decade. This catchy phrase indicates that users are seen not only as borrowers, but as active participants. The aim of this paper is to investigate and analyse three questions in relation to user...... participation in public libraries in a Nordic perspective. How can participation in public libraries be characterised? Why should libraries deal with user participation? What kinds of different user participation can be identified in public libraries? Design/methodology/approach The paper uses a selection...

  20. A public health perspective

    African Journals Online (AJOL)

    user

    EDITORIAL. Enabling local health departments to save more lives: A public ... promoting health through the organized efforts of society” (1) ... and synergistic with achieving the sustainable development goals because its furtherance brings a ...

  1. Publication point indicators

    DEFF Research Database (Denmark)

    Elleby, Anita; Ingwersen, Peter

    2010-01-01

    The paper presents comparative analyses of two publication point systems, The Norwegian and the in-house system from the interdiscplinary Danish Institute for International Studies (DIIS), used as case in the study for publications published 2006, and compares central citation-based indicators...... with novel publication point indicators (PPIs) that are formalized and exemplified. Two diachronic citation windows are applied: 2006-07 and 2006-08. Web of Science (WoS) as well as Google Scholar (GS) are applied to observe the cite delay and citedness for the different document types published by DIIS...... for all document types. Statistical significant correlations were only found between WoS and GS and the two publication point systems in between, respectively. The study demonstrates how the nCPPI can be applied to institutions as evaluation tools supplementary to JCI in various combinations...

  2. Ethics in Online Publications.

    Science.gov (United States)

    Vervaart, Peter

    2014-10-01

    Journals have been publishing the results of scientific investigations since the founding of Philosophical Transactions in 1665. Since then we have witnessed a massive expansion in the number of journals to the point that there are now approximately 28,000 active, peer reviewed journals collectively publishing more than 1.8 million articles per year. Before the mid-1990s, these journals were only available on paper but by the end of the 20th century, most journals had moved to online platforms. Online publication has also served as the impetus for the move to 'open-access' to the information contained in journals. The fact that a publication is 'on-line' and 'open-access' does not negate the responsibility of the author and the publisher to publish in an ethical way. [1] The document produced by the IFCC Ethics Task Force (TF-E) on publication ethics states that 'Ethics in Science at its broadest level encompasses research ethics, medical ethics, publication ethics, conflicts of interest, ethical responsibilities as educator, plus many other areas.' Thus publication ethics is a continuum from the first step of research design through to the information being read by the reader. In general terms 'publication ethics' includes the ethical behaviour of the authors in writing and submitting a scientific manuscript to a publisher for the purpose of publication, thus any discussion of publication ethics must include the role of the authors, referees, publisher and reader and the issues of authorship (and the use of 'ghosts'), plagiarism, duplicate publication (including in different languages), image manipulation (particularly in the era of digitisation), and conflict of interest [2]. To aid the authors, and others involved in the process of publication, a number of resources are now available particularly those from the Committee on Publication Ethics (COPE) [3] and the World Association of Medical Editors (WAME) [4]. More recently the issue of 'publisher ethics' has

  3. Playing it by the rules: Local bans on the public use of soft drugs and the production of shared spaces of everyday life

    NARCIS (Netherlands)

    Chevalier, D.A.M.

    2015-01-01

    In a nutshell, this book is about formalized social control in public space: it examines the different ways shared public spaces of everyday life are used and perceived, focusing on the manner in which the urge to control such space is operationalized, and how in turn formalized control effects the

  4. Bibliography of publications

    International Nuclear Information System (INIS)

    1984-01-01

    The list of publications of the Institute of Isotopes of the Hungarian Academy of Sciences covers a great variety of fields of activities performed by the members of the Institute. References are catalogized annually according to an alphabetical order of the authors. For a given author, Journal articles are followed by conference papers, and finally, by monographs. Authors employed by the Institute are distinguished from co-operators. A total of 1525 publications are involved. An author's index is attached. (V.N.)

  5. Lands directorate publications

    Energy Technology Data Exchange (ETDEWEB)

    1981-01-01

    The directorate has a lead role in providing advice to the federal government on land use policy in Canada. The Canada Land Inventory (CLI) Program has produced significant amounts of data pertaining to the capability of Canadian lands to support agriculture, forestry, recreation, wildlife and sport fish. A list of CLI reports is presented in this publication. In addition, and capability maps have been compiled for agricultural, forestry, recreation and wildlife and are listed and described in this publication. (KRM)

  6. Public Relations In Nigerian Public Libraries: A Critical Assessment ...

    African Journals Online (AJOL)

    This paper critically assesses public relations in public libraries in Nigeria. A simple random sampling method was used to select 751 staff and 1161 users from 14 chosen public libraries. The result shows that about 60% of staff in public libraries were unaware of the importance of public relation in the delivery of effective ...

  7. Opportunities for Public Relations Research in Public Health.

    Science.gov (United States)

    Wise, Kurt

    2001-01-01

    Considers how communication researchers have developed a solid body of knowledge in the health field but know little about the activities of public relations practitioners in public health bodies. Suggests that public relations scholarship and practice have much to offer the field of public health in helping public health bodies meet their…

  8. Problematizing Public Engagement within Public Pedagogy Research and Practice

    Science.gov (United States)

    Sandlin, Jennifer A.; Burdick, Jake; Rich, Emma

    2017-01-01

    In this article, we explore issues related to how scholars attempt to "enact public pedagogy" (i.e. doing "public engagement" work) and how they "research public pedagogy" (i.e. framing and researching artistic and activist "public engagement" as public pedagogy). We focus specifically on three interrelated…

  9. GIS and Public Health

    Directory of Open Access Journals (Sweden)

    Stefania Bertazzon

    2014-06-01

    Full Text Available This Special Issue on GIS and public health is the result of a highly selective process, which saw the participation of some 20 expert peer-reviewers and led to the acceptance of one half of the high-quality submissions received over the past year. Many threads link these papers to each other and, indeed, to our original call for papers, but the element that most clearly emerges from these works is the inextricable connection between public health and the environment. Indeed, GIS analysis of public health simply cannot disregard the geospatial dimension of environmental resources and risks. What consistently emerges from these analyses is that current geospatial research can only scratch the surface of the complex interactions of spatial resources, risks, and public health. In today’s world, or at least in the developed world, researchers and practitioners can count on virtually endless data, on inexpensive computational power, and on seamless connectivity. In this research environment, these papers point to the need for improved analytical tools, covering concepts, representation, modeling and reliability. These works are important contributions that help us to identify what advances in geospatial analysis can better address the complex interactions of public health with our physical and cultural environment, and bridge research and practice, so that geospatial analyses can inform public health policy making. [...

  10. Public acceptance and USCEA

    International Nuclear Information System (INIS)

    Anon.

    1993-01-01

    Public opinion plays a critical role in ensuring the future of nuclear energy. After the events at Three Mile Island in 1979, and more recently the Chernobyl accident, worldwide antinuclear movements surged upward. As a result, the global nuclear industry was compelled to institute education programs aimed at allaying public apprehension surrounding nuclear power. The US Council for Energy Awareness strives to disseminate information on nuclear issues in an effort to educate the public and thus create a foundation of support for this important energy option. The US Council for Energy Awareness (USCEA) is the national communications and information organization for the US commercial nuclear power industry. The organization provides a national voice for an industry whose issues are not always well understood by the public or by policy makers. It performs many functions for member companies, and offers information to the public. Included in the primary functions USCEA conducts on behalf of the nuclear industry are advertising, public and media relations, technical programs, and conferences. Worldwide, its membership is approximately 400, and includes utilities, vendors, consultants, universities, and other organizations with an interest in nuclear power

  11. STRATEGI KOMUNIKASI PUBLIC RELATIONS

    Directory of Open Access Journals (Sweden)

    Artis Artis

    2011-11-01

    Full Text Available Living man in interrelates society. Relationship among human being done by gets communication so man one by another one mutually understand and influence regard for the benefit, it that always been practiced by Public Relations( liaison in a governance and also firm institute to reach to the effect which ices. The institute of Public Relations declares for,”Public Relations is overall effort which be passed off by design and berkesenambungan in order to creates and pet keenness and mutual understanding among an organization with whole its member “. In this case, Public Relations in give distribution on manajement's strategy there is two: First, Doing task as part of Public Relations's strategy and involvement in comprehensive process and gives benefit for management an organization. Both of, Public Relations gets role in management strategy in bring off activity to reach to the effect good one gets internal character and also external one correspond to that desirable by good institute on level governance and firm.

  12. MiR-132 regulates osteogenic differentiation via downregulating Sirtuin1 in a peroxisome proliferator-activated receptor β/δ–dependent manner

    Energy Technology Data Exchange (ETDEWEB)

    Gong, Kai; Qu, Bo; Liao, Dongfa; Liu, Da; Wang, Cairu; Zhou, Jingsong; Pan, Xianming, E-mail: xianmingpanxj@163.com

    2016-09-09

    MicroRNAs (miRNAs) play significant roles in multiple diseases by regulating the expression of their target genes. Type 2 diabetes mellitus (T2DM) is a chronic endocrine and metabolic disease with complex mechanisms. T2DM can result in diabetic osteoporosis (DO), which is characterized by bone loss, decreased bone mineral density and increased bone fractures. The promotion of osteogenic differentiation of osteoblasts is an effective way to treat osteoporosis. In the present study, high glucose (HG) and free fatty acids (FFA) were employed to mimic T2DM in MC3T3-E1 cells. To induce osteogenic differentiation, MC3T3-E1 cells were cultured in osteogenic medium. The results showed that osteogenic differentiation was significantly suppressed by HG and FFA. We found that miR-132 expression was significantly upregulated and much higher in HG-FFA–induced cells than other selected miRNAs, indicating that miR-132 might play an important role in DO. Furthermore, overexpression of miR-132 markedly inhibited the expression of key markers of osteogenic differentiation and alkaline phosphatase (ALP) activity. Reciprocally, inhibition of miR-132 restored osteogenic differentiation, even under treatment with HG-FFA. We also showed that Sirtuin 1 (Sirt1) was one of the target genes of miR-132, whose expression was controlled by miR-132. Ectopic expression of Sirt1 reversed the decrease in osteogenic differentiation caused by miR-132 and HG-FFA. These results demonstrated the direct role of miR-132 in suppressing osteogenic differentiation through downregulating Sirt1. Moreover, we demonstrated that peroxisome proliferator-activated receptor β/δ (PPARβ/δ) was a downstream molecule of Sirt1, and its knockout by PPARβ/δ siRNA significantly abolished the promotive effects of Sirt1 on osteogenic differentiation, indicating that Sirt1 functioned in a PPARβ/δ–dependent manner. Taken together, we provide crucial evidence that miR-132 plays a key role in regulating osteogenic

  13. A novel small molecule FL118 that selectively inhibits survivin, Mcl-1, XIAP and cIAP2 in a p53-independent manner, shows superior antitumor activity.

    Directory of Open Access Journals (Sweden)

    Xiang Ling

    Full Text Available Drug/radiation resistance to treatment and tumor relapse are major obstacles in identifying a cure for cancer. Development of novel agents that address these challenges would therefore be of the upmost importance in the fight against cancer. In this regard, studies show that the antiapoptotic protein survivin is a central molecule involved in both hurdles. Using cancer cell-based survivin-reporter systems (US 7,569,221 B2 via high throughput screening (HTS of compound libraries, followed by in vitro and in vivo analyses of HTS-derived hit-lead compounds, we identified a novel anticancer compound (designated FL118. FL118 shows structural similarity to irinotecan. However, while the inhibition of DNA topoisomerase 1 activity by FL118 was no better than the active form of irinotecan, SN-38 at 1 µM, FL118 effectively inhibited cancer cell growth at less than nM levels in a p53 status-independent manner. Moreover, FL118 selectively inhibited survivin promoter activity and gene expression also in a p53 status-independent manner. Although the survivin promoter-reporter system was used for the identification of FL118, our studies revealed that FL118 not only inhibits survivin expression but also selectively and independently inhibits three additional cancer-associated survival genes (Mcl-1, XIAP and cIAP2 in a p53 status-independent manner, while showing no inhibitory effects on control genes. Genetic silencing or overexpression of FL118 targets demonstrated a role for these targets in FL118's effects. Follow-up in vivo studies revealed that FL118 exhibits superior antitumor efficacy in human tumor xenograft models in comparison with irinotecan, topotecan, doxorubicin, 5-FU, gemcitabine, docetaxel, oxaliplatin, cytoxan and cisplatin, and a majority of mice treated with FL118 showed tumor regression with a weekly × 4 schedule. FL118 induced favorable body-weight-loss profiles (temporary and reversible and was able to eliminate large tumors. Together

  14. MiR-132 regulates osteogenic differentiation via downregulating Sirtuin1 in a peroxisome proliferator-activated receptor β/δ–dependent manner

    International Nuclear Information System (INIS)

    Gong, Kai; Qu, Bo; Liao, Dongfa; Liu, Da; Wang, Cairu; Zhou, Jingsong; Pan, Xianming

    2016-01-01

    MicroRNAs (miRNAs) play significant roles in multiple diseases by regulating the expression of their target genes. Type 2 diabetes mellitus (T2DM) is a chronic endocrine and metabolic disease with complex mechanisms. T2DM can result in diabetic osteoporosis (DO), which is characterized by bone loss, decreased bone mineral density and increased bone fractures. The promotion of osteogenic differentiation of osteoblasts is an effective way to treat osteoporosis. In the present study, high glucose (HG) and free fatty acids (FFA) were employed to mimic T2DM in MC3T3-E1 cells. To induce osteogenic differentiation, MC3T3-E1 cells were cultured in osteogenic medium. The results showed that osteogenic differentiation was significantly suppressed by HG and FFA. We found that miR-132 expression was significantly upregulated and much higher in HG-FFA–induced cells than other selected miRNAs, indicating that miR-132 might play an important role in DO. Furthermore, overexpression of miR-132 markedly inhibited the expression of key markers of osteogenic differentiation and alkaline phosphatase (ALP) activity. Reciprocally, inhibition of miR-132 restored osteogenic differentiation, even under treatment with HG-FFA. We also showed that Sirtuin 1 (Sirt1) was one of the target genes of miR-132, whose expression was controlled by miR-132. Ectopic expression of Sirt1 reversed the decrease in osteogenic differentiation caused by miR-132 and HG-FFA. These results demonstrated the direct role of miR-132 in suppressing osteogenic differentiation through downregulating Sirt1. Moreover, we demonstrated that peroxisome proliferator-activated receptor β/δ (PPARβ/δ) was a downstream molecule of Sirt1, and its knockout by PPARβ/δ siRNA significantly abolished the promotive effects of Sirt1 on osteogenic differentiation, indicating that Sirt1 functioned in a PPARβ/δ–dependent manner. Taken together, we provide crucial evidence that miR-132 plays a key role in regulating osteogenic

  15. AtPP2CG1, a protein phosphatase 2C, positively regulates salt tolerance of Arabidopsis in abscisic acid-dependent manner

    Energy Technology Data Exchange (ETDEWEB)

    Liu, Xin, E-mail: fangfei6073@126.com [Plant Bioengineering Laboratory, Northeast Agricultural University, Harbin 150030 (China); Zhu, Yanming, E-mail: ymzhu2001@neau.edu.cn [Plant Bioengineering Laboratory, Northeast Agricultural University, Harbin 150030 (China); Zhai, Hong, E-mail: Zhai.h@neigaehrb.ac.cn [Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin 150040 (China); Cai, Hua, E-mail: small-big@sohu.com [Plant Bioengineering Laboratory, Northeast Agricultural University, Harbin 150030 (China); Ji, Wei, E-mail: iwei_j@hotmail.com [Plant Bioengineering Laboratory, Northeast Agricultural University, Harbin 150030 (China); Luo, Xiao, E-mail: luoxiao2010@yahoo.cn [Plant Bioengineering Laboratory, Northeast Agricultural University, Harbin 150030 (China); Li, Jing, E-mail: lijing@neau.edu.cn [Plant Secondary Metabolism Laboratory, Northeast Agricultural University, Harbin 150030 (China); Bai, Xi, E-mail: baixi@neau.edu.cn [Plant Bioengineering Laboratory, Northeast Agricultural University, Harbin 150030 (China)

    2012-06-15

    Highlights: Black-Right-Pointing-Pointer AtPP2CG1 positively regulates salt tolerance in ABA-dependent manner. Black-Right-Pointing-Pointer AtPP2CG1 up-regulates the expression of marker genes in different pathways. Black-Right-Pointing-Pointer AtPP2CG1 expresses in the vascular system and trichomes of Arabidopsis. -- Abstract: AtPP2CG1 (Arabidopsis thaliana protein phosphatase 2C G Group 1) was predicted as an abiotic stress candidate gene by bioinformatic analysis in our previous study. The gene encodes a putative protein phosphatase 2C that belongs to Group G of PP2C. There is no report of Group G genes involved in abiotic stress so far. Real-time RT-PCR analysis showed that AtPP2CG1 expression was induced by salt, drought, and abscisic acid (ABA) treatment. The expression levels of AtPP2CG1 in the ABA synthesis-deficient mutant abi2-3 were much lower than that in WT plants under salt stress suggesting that the expression of AtPP2CG1 acts in an ABA-dependent manner. Over-expression of AtPP2CG1 led to enhanced salt tolerance, whereas its loss of function caused decreased salt tolerance. These results indicate that AtPP2CG1 positively regulates salt stress in an ABA-dependent manner. Under salt treatment, AtPP2CG1 up-regulated the expression levels of stress-responsive genes, including RD29A, RD29B, DREB2A and KIN1. GUS activity was detected in roots, leaves, stems, flower, and trichomes of AtPP2CG1 promoter-GUS transgenic plants. AtPP2CG1 protein was localized in nucleus and cytoplasm via AtPP2CG1:eGFP and YFP:AtPP2CG1 fusion approaches.

  16. RELATIONS WITH THE PUBLIC VERSUS PUBLIC RELATIONS IN LOCAL PUBLIC ADMINISTRATION

    Directory of Open Access Journals (Sweden)

    Bente Florina Maria

    2010-12-01

    Full Text Available Concomitant with the evolution of society, public relations are socially expressed only together with the explicit articulation of public categories and public organizations, once the individual becomes a citizen whose satisfaction is at the core of the public systems preoccupations, ignoring times long gone when the ordinary citizen and the majority of the public administration representatives couldnt tell apart the concept of public relations from that of relations with the public.

  17. Public healthcare interests require strict competition enforcement.

    Science.gov (United States)

    Loozen, Edith M H

    2015-07-01

    Several countries have introduced competition in their health systems in order to maintain the supply of high quality health care in a cost-effective manner. The introduction of competition triggers competition enforcement. Since healthcare is characterized by specific market failures, many favor healthcare-specific competition enforcement in order not only to account for the competition interest, but also for the healthcare interests. The question is whether healthcare systems based on competition can succeed when competition enforcement deviates from standard practice. This paper analyzes whether healthcare-specific competition enforcement is theoretically sound and practically effective. This is exemplified by the Dutch system that is based on regulated competition and thus crucially depends on getting competition enforcement right. Governments are responsible for correcting market failures. Markets are responsible for maximizing the public healthcare interests. By securing sufficient competitive pressure, competition enforcement makes sure they do. When interpreted according to welfare-economics, competition law takes into account both costs and benefits specific market behavior may have for healthcare. Competition agencies and judiciary are not legitimized to deviate from standard evidentiary requirements. Dutch case law shows that healthcare-specific enforcement favors the healthcare undertakings concerned, but to the detriment of public health care. Healthcare-specific competition enforcement is conceptually flawed and counterproductive. In order for healthcare systems based on competition to succeed, competition enforcement should be strict. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

  18. Public Finance Administration. Second Edition.

    Science.gov (United States)

    Reed, B. J.; Swain, John W.

    This book is intended for the nonexpert in finance who has a public administration background. It opens with a broad introduction to public finance administration and how this job is related to public budgeting, the practice of public-sector accounting, and the economic concepts of money and value. Issues surrounding public revenue, its sources,…

  19. Women in public life.

    Science.gov (United States)

    1992-01-01

    The UN Division for the Advancement of Women publication has devoted an issue to the role of women in public lie based on an analysis of women's status in industrialized countries presented in Vienna, Austria, in May 1991. Women already contribute to political life and make a difference in politics, but societal institutions and government processes have not yet adapted to this fact. Women's nongovernmental organizations promote women's interests at the governmental level, but often do not have the economic or political power as do other interests groups such as trade unions. Women often participation public life via their membership in women's organizations, community action groups, voluntary organizations, and other close to home groups. They prefer to participate in activities which are problem solving rather than institution building. These activities and groups operate outside established political institutions and are not considered as part of public and political life. Society's exclusion of women from leadership positions in public life keeps it from benefiting from the special contributions that women bring to decision making. Women show a tendency to have different leadership styles than men (e.g., ability to relate to people affected by their decisions), which are most needed for the modern world. They often do not campaign just for women's issues, but, once in office, they do tend to become more involved in women's issues. Women have affected positive changes in career and child care, often on a non-Socialist agenda, in various countries (e.g. Norway). This effect is referred to as the politics of motherhood. More access to politics and public life calls for removal of structural and situational barriers including the glass ceiling, discrimination, insufficient funds, and bearing most of the responsibility for child care. The UN women's groups has drafted a platform for interregional consultation on women's role in public life and scheduled the 4th

  20. A short review of radiation-induced raft-mediated graft copolymerization: A powerful combination for modifying the surface properties of polymers in a controlled manner

    Science.gov (United States)

    Barsbay, Murat; Güven, Olgun

    2009-12-01

    Surface grafting of polymeric materials is attracting increasing attention as it enables the preparation of new materials from known and commercially available polymers having desirable bulk properties such as thermal stability, elasticity, permeability, etc., in conjunction with advantageous newly tailored surface properties such as biocompatibility, biomimicry, adhesion, etc. Ionizing radiation, particularly γ radiation is one of the most powerful tools for preparing graft copolymers as it generates radicals on most substrates. With the advent of living free-radical polymerization techniques, application of γ radiation has been extended to a new era of grafting; grafting in a controlled manner to achieve surfaces with tailored and well-defined properties. This report presents the current use of γ radiation in living free-radical polymerization and highlights the use of both techniques together as a combination to present an advance in the ability to prepare surfaces with desired, tunable and well-defined properties.

  1. A short review of radiation-induced raft-mediated graft copolymerization: A powerful combination for modifying the surface properties of polymers in a controlled manner

    International Nuclear Information System (INIS)

    Barsbay, Murat; Gueven, Olgun

    2009-01-01

    Surface grafting of polymeric materials is attracting increasing attention as it enables the preparation of new materials from known and commercially available polymers having desirable bulk properties such as thermal stability, elasticity, permeability, etc., in conjunction with advantageous newly tailored surface properties such as biocompatibility, biomimicry, adhesion, etc. Ionizing radiation, particularly γ radiation is one of the most powerful tools for preparing graft copolymers as it generates radicals on most substrates. With the advent of living free-radical polymerization techniques, application of γ radiation has been extended to a new era of grafting; grafting in a controlled manner to achieve surfaces with tailored and well-defined properties. This report presents the current use of γ radiation in living free-radical polymerization and highlights the use of both techniques together as a combination to present an advance in the ability to prepare surfaces with desired, tunable and well-defined properties.

  2. A short review of radiation-induced raft-mediated graft copolymerization: A powerful combination for modifying the surface properties of polymers in a controlled manner

    Energy Technology Data Exchange (ETDEWEB)

    Barsbay, Murat [Department of Chemistry, Hacettepe University, 06800 Beytepe, Ankara (Turkey)], E-mail: mbarsbay@hacettepe.edu.tr; Gueven, Olgun [Department of Chemistry, Hacettepe University, 06800 Beytepe, Ankara (Turkey)], E-mail: guven@hacettepe.edu.tr

    2009-12-15

    Surface grafting of polymeric materials is attracting increasing attention as it enables the preparation of new materials from known and commercially available polymers having desirable bulk properties such as thermal stability, elasticity, permeability, etc., in conjunction with advantageous newly tailored surface properties such as biocompatibility, biomimicry, adhesion, etc. Ionizing radiation, particularly {gamma} radiation is one of the most powerful tools for preparing graft copolymers as it generates radicals on most substrates. With the advent of living free-radical polymerization techniques, application of {gamma} radiation has been extended to a new era of grafting; grafting in a controlled manner to achieve surfaces with tailored and well-defined properties. This report presents the current use of {gamma} radiation in living free-radical polymerization and highlights the use of both techniques together as a combination to present an advance in the ability to prepare surfaces with desired, tunable and well-defined properties.

  3. Meeting the Challenge of Ebola Virus Disease in a Holistic Manner by Taking into Account Socioeconomic and Cultural Factors: The Experience of West Africa

    Directory of Open Access Journals (Sweden)

    Kai-Lit Phua

    2015-01-01

    Full Text Available Even if an effective vaccine against Ebola virus disease (EVD becomes available, the challenges posed by this disease are complex. Certain socioeconomic and cultural factors have been linked to recent outbreaks of EVD in West Africa. The outbreaks revealed widespread ignorance by laypersons of EVD etiology, mode of transmission, and personal protective measures that can be taken. Lack of trust in the authorities, virus infection during the preparation of “bushmeat” for human consumption, traditional funerary practices, and relatively free flow of goods and people between regions and across international borders may have facilitated the spread of EVD and hindered outbreak control efforts. Inadequacy in health systems of the most seriously affected countries, such as Guinea, Sierra Leone, and Liberia, is also an important factor. The objectives of this article are to argue that EVD should be evaluated in a systematic and holistic manner and that this can be done through the use of the modified Haddon Matrix.

  4. The Atlantic Ocean and the “Mighty Rivers” of the New World: Natural Phenomena and Human Encounters in Frances Trollope’s Domestic Manners of t

    OpenAIRE

    D’AMORE, Manuela

    2014-01-01

    L’objectif de cette étude est de présenter une lecture de Domestic Manners of the Americans (1832), le premier best-seller de Frances Trollope (1780-1863), qui montre les liens importants entre cet œuvre et les « Sea » et « Oceanic Studies ». Le rapport conflictuel avec l’Autre, la représentation de Soi comme une courageuse « lady » anglaise dans le Nouveau Monde des années 1820, les différentes étapes de son voyage et les expériences de la navigation démontreront au lecteur contemporain que,...

  5. Resistible force. Purpose and manner of the protest against nuclear energy. Der aufhaltsame Zwang. Sinn und Wege des Widerstands gegen Kernenergie

    Energy Technology Data Exchange (ETDEWEB)

    Baeschlin, D.L.

    1980-01-01

    Many people of today have an oppositional attitude towards the continual loss of sense of existence which manifests itself in 'the great refusal'. They also behave in an anti-authoritarian manner towards their first father Marx. They realize that it is ourselves who have to search and find. The protest groups of the most different origin have come into being as 'the sand in the wheels' of a seemingly irresistable force. They have formed themselves beyond the 'Left' and the 'Right'. Nuclear energy is the instrument which acts as a gear for their opposition, which establishes it and which provides the basis for their self-concept. It is the symbol where the conflict between living standard and living quality breaks open. In essential, however, the protest is growing towards supporting an effective administration of all goods of our world.

  6. Public health and peace.

    Science.gov (United States)

    Laaser, Ulrich; Donev, Donco; Bjegović, Vesna; Sarolli, Ylli

    2002-04-01

    The modern concept of public health, the New Public Health, carries a great potential for healthy and therefore less aggressive societies. Its core disciplines are health promotion, environmental health, and health care management based on advanced epidemiological methodologies. The main principles of living together in healthy societies can be summarized as four ethical concepts of the New Public Health essential to violence reduction equity, participation, subsidiarity, and sustainability. The following issues are discussed as violence determinants: the process of urbanization; type of neighborhood and accommodation, and consequent stigmatization; level of education; employment status; socialization of the family; women's status; alcohol and drug consumption; availability of the firearms; religious, ethnic, and racial prejudices; and poverty. Development of the health systems has to contribute to peace, since aggression, violence, and warfare are among the greatest risks for health and the economic welfare. This contribution can be described as follows: 1) full and indiscriminate access to all necessary services, 2) monitoring of their quality, 3) providing special support to vulnerable groups, and 4) constant scientific and public accountability of the evaluation of the epidemiological outcome. Violence can also destroy solidarity and social cohesion of groups, such as family, team, neighborhood, or any other social organization. Durkheim coined the term anomie for a state in which social disruption of the community results in health risks for individuals. Health professionals can make a threefold contribution to peace by 1) analyzing the causal interrelationships of violence phenomena, 2) curbing the determinants of violence according to the professional standards, and 3) training professionals for this increasingly important task. Because tolerance is an essential part of an amended definition of health, monitoring of the early signs of public intolerance is

  7. 3-[2,4-Dimethoxybenzylidene]anabaseine (DMXB) selectively activates rat alpha7 receptors and improves memory-related behaviors in a mecamylamine-sensitive manner.

    Science.gov (United States)

    Meyer, E M; Tay, E T; Papke, R L; Meyers, C; Huang, G L; de Fiebre, C M

    1997-09-12

    The alpha7 nicotinic receptor agonist 3-[2,4-dimethoxybenzylidene]anabaseine (DMXB; GTS-21) was investigated for its ability to: (1) activate a variety of nicotinic receptor subtypes in Xenopus oocytes; (2) improve passive avoidance and spatial Morris water task performances in mecamylamine-sensitive manners in bilaterally nucleus basalis lesioned rats; and (3) elevate high-affinity [3H]acetylcholine (ACh) and high-affinity alpha-[125I]bungarotoxin binding in rat neocortex following 2 weeks of daily injections. DMXB (100 microM) activated alpha7 homo-oligomeric receptors, without significant activity at alpha2-, alpha3- and alpha4-containing subtypes. Mecamylamine blocked rat alpha7 receptors weakly if co-administered with agonist, but much more potently when pre-applied. Bilateral ibotenic acid lesions of the nucleus basalis interfered with passive avoidance and spatial memory-related behaviors. DMXB (0.5 mg/kg, i.p.) improved passive avoidance behavior in lesioned animals in a mecamylamine-sensitive manner. DMXB (0.5 mg/kg 15 min before each session) also improved performance in the training and probe components of the Morris water task. DMXB-induced improvement in the probe component but not the training phase was mecamylamine-sensitive. [3H]ACh binding was elevated after 14 days of daily i.p. injections with 0.2 mg/kg nicotine but not after 1 mg/kg DMXB. Neither drug elevated high-affinity alpha-[125I]bungarorotoxin binding over this interval.

  8. Regulation of AKT phosphorylation at Ser473 and Thr308 by endoplasmic reticulum stress modulates substrate specificity in a severity dependent manner.

    Directory of Open Access Journals (Sweden)

    Hong Wa Yung

    2011-03-01

    Full Text Available Endoplasmic reticulum (ER stress is a common factor in the pathophysiology of diverse human diseases that are characterised by contrasting cellular behaviours, from proliferation in cancer to apoptosis in neurodegenerative disorders. Coincidently, dysregulation of AKT/PKB activity, which is the central regulator of cell growth, proliferation and survival, is often associated with the same diseases. Here, we demonstrate that ER stress modulates AKT substrate specificity in a severity-dependent manner, as shown by phospho-specific antibodies against known AKT targets. ER stress also reduces both total and phosphorylated AKT in a severity-dependent manner, without affecting activity of the upstream kinase PDK1. Normalisation to total AKT revealed that under ER stress phosphorylation of Thr308 is suppressed while that of Ser473 is increased. ER stress induces GRP78, and siRNA-mediated knock-down of GRP78 enhances phosphorylation at Ser473 by 3.6 fold, but not at Thr308. Substrate specificity is again altered. An in-situ proximity ligation assay revealed a physical interaction between GRP78 and AKT at the plasma membrane of cells following induction of ER stress. Staining was weak in cells with normal nuclear morphology but stronger in those displaying rounded, condensed nuclei. Co-immunoprecipitation of GRP78 and P-AKT(Ser473 confirmed the immuno-complex consists of non-phosphorylated AKT (Ser473 and Thr308. The interaction is likely specific as AKT did not bind to all molecular chaperones, and GRP78 did not bind to p70 S6 kinase. These findings provide one mechanistic explanation for how ER stress contributes to human pathologies demonstrating contrasting cell fates via modulation of AKT signalling.

  9. Iron oxide magnetic nanoparticles combined with actein suppress non-small-cell lung cancer growth in a p53-dependent manner

    Directory of Open Access Journals (Sweden)

    Wang MS

    2017-10-01

    Full Text Available Ming-Shan Wang,1 Liang Chen,2 Ya-Qiong Xiong,2 Jing Xu,2 Ji-Peng Wang,2 Zi-Li Meng2 1Department of Oncology, Huaiyin Hospital of Huai’an City, Huai’an, China; 2Department of Respiration, Huai’an First People’s Hospital, Nanjing Medical University, Huai’an, China Abstract: Actein (AT is a triterpene glycoside isolated from the rhizomes of Cimicifuga foetida that has been investigated for its antitumor effects. AT treatment leads to apoptosis in various cell types, including breast cancer cells, by regulating different signaling pathways. Iron oxide (Fe3O4 magnetic nanoparticles (MNPs are nanomaterials with biocompatible activity and low toxicity. In the present study, the possible benefits of AT in combination with MNPs on non-small-cell lung cancer (NSCLC were explored in in vitro and in vivo studies. AT-MNP treatment contributed to apoptosis in NSCLC cells, as evidenced by activation of the caspase 3-signaling pathway, which was accompanied by downregulation of the antiapoptotic proteins Bcl2 and BclXL, and upregulation of the proapoptotic signals Bax and Bad. The death receptors of TRAIL were also elevated following AT-MNP treatment in a p53-dependent manner. Furthermore, a mouse xenograft model in vivo revealed that AT-MNP treatment exhibited no toxicity and suppressed NSCLC growth compared to either AT or MNP monotherapies. In conclusion, this study suggests a novel therapy to induce apoptosis in suppressing NSCLC growth in a p53-dependent manner by combining AT with Fe3O4 MNPs. Keywords: actein, Fe3O4 magnetic nanoparticles, NSCLC, apoptosis, p53

  10. Cisplatin-resistant lung cancer cell-derived exosomes increase cisplatin resistance of recipient cells in exosomal miR-100-5p-dependent manner.

    Science.gov (United States)

    Qin, Xiaobing; Yu, Shaorong; Zhou, Leilei; Shi, Meiqi; Hu, Yong; Xu, Xiaoyue; Shen, Bo; Liu, Siwen; Yan, Dali; Feng, Jifeng

    2017-01-01

    Exosomes derived from lung cancer cells confer cisplatin (DDP) resistance to other cancer cells. However, the underlying mechanism is still unknown. A549 resistance to DDP (A549/DDP) was established. Microarray was used to analyze microRNA (miRNA) expression profiles of A549 cells, A549/DDP cells, A549 exosomes, and A549/DDP exosomes. There was a strong correlation of miRNA profiles between exosomes and their maternal cells. A total of 11 miRNAs were significantly upregulated both in A549/DDP cells compared with A549 cells and in exosomes derived from A549/DDP cells in contrast to exosomes from A549 cells. A total of 31 downregulated miRNAs were also observed. miR-100-5p was the most prominent decreased miRNA in DDP-resistant exosomes compared with the corresponding sensitive ones. Downregulated miR-100-5p was proved to be involved in DDP resistance in A549 cells, and mammalian target of rapamycin (mTOR) expression was reverse regulated by miR-100-5p. Exosomes confer recipient cells' resistance to DDP in an exosomal miR-100-5p-dependent manner with mTOR as its potential target both in vitro and in vivo. Exosomes from DDP-resistant lung cancer cells A549 can alter other lung cancer cells' sensitivity to DDP in exosomal miR-100-5p-dependent manner. Our study provides new insights into the molecular mechanism of DDP resistance in lung cancer.

  11. Regulation of AKT Phosphorylation at Ser473 and Thr308 by Endoplasmic Reticulum Stress Modulates Substrate Specificity in a Severity Dependent Manner

    Science.gov (United States)

    Yung, Hong Wa

    2011-01-01

    Endoplasmic reticulum (ER) stress is a common factor in the pathophysiology of diverse human diseases that are characterised by contrasting cellular behaviours, from proliferation in cancer to apoptosis in neurodegenerative disorders. Coincidently, dysregulation of AKT/PKB activity, which is the central regulator of cell growth, proliferation and survival, is often associated with the same diseases. Here, we demonstrate that ER stress modulates AKT substrate specificity in a severity-dependent manner, as shown by phospho-specific antibodies against known AKT targets. ER stress also reduces both total and phosphorylated AKT in a severity-dependent manner, without affecting activity of the upstream kinase PDK1. Normalisation to total AKT revealed that under ER stress phosphorylation of Thr308 is suppressed while that of Ser473 is increased. ER stress induces GRP78, and siRNA-mediated knock-down of GRP78 enhances phosphorylation at Ser473 by 3.6 fold, but not at Thr308. Substrate specificity is again altered. An in-situ proximity ligation assay revealed a physical interaction between GRP78 and AKT at the plasma membrane of cells following induction of ER stress. Staining was weak in cells with normal nuclear morphology but stronger in those displaying rounded, condensed nuclei. Co-immunoprecipitation of GRP78 and P-AKT(Ser473) confirmed the immuno-complex consists of non-phosphorylated AKT (Ser473 and Thr308). The interaction is likely specific as AKT did not bind to all molecular chaperones, and GRP78 did not bind to p70 S6 kinase. These findings provide one mechanistic explanation for how ER stress contributes to human pathologies demonstrating contrasting cell fates via modulation of AKT signalling. PMID:21445305

  12. Bisphenol A impairs the memory function and glutamatergic homeostasis in a sex-dependent manner in mice: Beneficial effects of diphenyl diselenide.

    Science.gov (United States)

    Jardim, Natália S; Sartori, Glaúbia; Sari, Marcel H M; Müller, Sabrina G; Nogueira, Cristina W

    2017-08-15

    Bisphenol A (BPA) is a compound integrated in commodities, which consequently increases the human exposure to this toxicant. The deleterious effects of BPA exposure during periods of brain development have been documented mainly concerning the impairment in memory functions. Diphenyl diselenide (PhSe) 2 , an organoselenium compound, shows protective/restorative effects against memory deficits in experimental models. Thus, this study investigated the effects of (PhSe) 2 on the memory impairments induced by BPA exposure to male and female mice and the possible involvement of glutamatergic system in these effects. Three-week-old male and female Swiss mice received BPA (5mg/kg), intragastrically, from 21st to 60th postnatal day. After, the animals were intragastrically treated with (PhSe) 2 (1mg/kg) during seven days. The mice performed the behavioral memory tests and the [ 3 H] glutamate uptake and NMDA receptor subunits (2A and 2B) analyses were carried out in the hippocampus and cerebral cortex of mice. The results demonstrated that the BPA exposure induced impairment of object recognition memory in both sexes. However, it caused impairments in spatial memory in female and in the passive avoidance memory in male mice. Besides, BPA caused a decrease in the [ 3 H] glutamate uptake and NMDA receptor subunit levels in the cortical and hippocampal regions depending on the sex. Treatment with (PhSe) 2 reversed in a sex-independent manner the behavioral impairments and molecular alterations. In conclusion, BPA had a negative effect in different memory types as well as in the glutamatergic parameters in a sex-dependent manner and (PhSe) 2 treatment was effective against these alterations. Copyright © 2017 Elsevier Inc. All rights reserved.

  13. Bone mineral density-associated polymorphisms are associated with obesity-related traits in Korean adults in a sex-dependent manner.

    Directory of Open Access Journals (Sweden)

    Seongwon Cha

    Full Text Available Obesity and osteoporosis share common physiological factors, including the presence of atherosclerosis, a risk factor for cardiometabolic disease, as well as a common progenitor that differentiates into both adipocytes and osteoblasts. Among the 23 polymorphisms associated with bone mineral density (BMD in recent genome-wide association studies (GWASs, an Osterix polymorphism has been identified and associated with childhood obesity in girls. Therefore, we focused on elucidating polymorphisms associated with adulthood obesity in a sex-dependent manner among the previously published BMD-associated polymorphisms from GWASs. We performed 2 screenings of 18 BMD-associated polymorphisms for obesity-related traits in 2,362 adults aged >20 years. We excluded 13 polymorphisms showing deviations from Hardy-Weinberg equilibrium or no association with obesity-related traits (body mass index, waist circumference (WC, and waist-to-hip ratio. Among 5 selected polymorphisms (rs9594738 of RANKL, rs17066364 of NUFIP1, rs7227401 of OSBPL1A, and rs1856057 and rs2982573 of ESR1 analyzed, 2 polymorphisms (rs9594738 and rs17066364 were associated with obesity-related traits. We found sex-dependent associations such that the 4 polymorphisms (excluding rs9594738 of RANKL were associated with abdominal traits such as WC and waist-to-hip ratio only in men. In addition, when the combined genetic risk score (GRS for WC increase was calculated with 4 SNPs (rs9594738, rs17066364, rs7227401, and rs1856057 exhibiting similar trends for both sexes, the magnitude of the GRS effect for the WC increase was larger in men than in women (effect size = 0.856 cm, P = 0.0000452 for men; effect size = 0.598 cm, P = 0.00228 for women. In summary, we found 4 polymorphisms, previously related to osteoporosis, to be associated to obesity-related traits in a sex-dependent manner in Korean adults, particularly in men.

  14. Do risk factors for problem behaviour act in a cumulative manner? An examination of ethnic minority and majority children through an ecological perspective.

    Science.gov (United States)

    Atzaba-Poria, Naama; Pike, Alison; Deater-Deckard, Kirby

    2004-05-01

    Extensive research has identified risk factors for problem behaviour in childhood. However, most of this research has focused on isolated variables, ignoring possible additive influences. The purpose of this study was to examine whether risk factors for problem behaviour act in a cumulative manner, and to investigate whether cumulative risk stemming from distinct ecological levels (Bronfenbrenner, 1979) differentially influences the manifestation of problem behaviours in middle childhood. In addition, ethnic differences between minority (i.e., Indian) and majority (i.e., English) families were examined. The sample consisted of 125 children (59 English and 66 of Indian origin) between the ages of 7 and 9.6 (M = 8.51, SD = 0.62) and their parents. Both mothers and fathers completed questionnaires regarding the children's problem behaviour and provided reports of the children's characteristics and environment. Children were also assessed and provided reports about themselves and their relationships. Finally, parent-child mutuality and parenting behaviour were coded from a videotaped parent-child interaction task. Risk factors acted in a cumulative manner - the more risk children experienced, the more problem behaviour they exhibited. Total problem behaviour was predicted by all three levels: individual, microsystem and exosystem. However, externalising problems were mainly predicted by microsystem-level cumulative risk, whereas internalising problems were predicted by both individual-level cumulative risk and exosystem-level cumulative risk. These results were similar for both ethnic groups. The support for the cumulative hypothesis highlights the importance of having a broad picture of children's characteristics and environmental components when analysing children's adjustment. The distinct influence of risk stemming from the different ecological levels suggests that the trajectories of internalising, externalising and total problem behaviour may be different.

  15. Honduras - Public Financial Management and Public-Private Partnerships

    Data.gov (United States)

    Millennium Challenge Corporation — The Honduras Threshold Country Program (TCP) aims to increase the efficiency and transparency of public financial management (PFM) and public private partnerships...

  16. Ethics in Public Administration

    Directory of Open Access Journals (Sweden)

    MSc. Arjeta Hallunovi

    2014-06-01

    Full Text Available The goal of this study is to deal with some ethical causes in the public administration, that aim the avoidance of negative phenomenon’s as theft, corruption, etc. In this direction, the debates about ethics are becoming more and more a global tendency, as an implement through which would be found the way to get out of the crisis. The success in the reforms of the public administrations in major part depends, on the public functionaries and their willingness to make fundamental changes in the way they work. In this direction, the State should strengthen and modernize the public service, should realize an employment system, which should reflect more the merits on its service. For this reason, the government’s attempts should be concentrated on the construction of a positive image for the state and its administration as professional objective, which is oriented to the services. Being aware for the practical restrictions of this study, we firstly chose to concentrate on the local public administrations ethics. The study will be focused on the practical comparative analysis of the city of Shkodra and Durres by the realization of a questionnaire by each of these municipalities and the Agency of Legalization and Urbanization of Informal Zones (ALUIZNI in Shkodra.

  17. DOE headquarters publications

    Energy Technology Data Exchange (ETDEWEB)

    None

    1978-09-01

    This bibliography provides listings of (mainly policy and programmatic) publications issued from the U.S. Department of Energy, Washington, D.C. The listings are arranged by the ''report code'' assigned to each of the major organizations at DOE Headquarters, followed by the three categories of environmental reports issued from DOE Headquarters. All of the publications listed, except for those shown as still ''in preparation,'' may be seen in the Energy Library. A title index arranged by title keywords follows the listings. Certain publications are omitted. They include such items as pamphlets, ''fact sheets,'' bulletins and weekly/monthly issuances of DOE's Energy Information Administration and Economic Regulatory Administration, and employee bulletins and newsletters. Omitted from the bibliography altogether are headquarters publications assigned other types of report codes--e.g., ''HCP'' (Headquarters Contractor Publication) and ''CONF'' (conference proceedings). (RWR)

  18. Prevention of Youth Violence: A Public Health Approach.

    Science.gov (United States)

    Sood, Aradhana Bela; Berkowitz, Steven J

    2016-04-01

    The causes of youth violence are multifactorial and include biological, individual, familial, social, and economic factors. The influence of parents, family members, and important adults can shape the beliefs of the child toward violence in a significant manner. However, the influence of school and the neighborhood also have an important role in attitudes and behaviors of children toward violence. The complexity of factors related to violence requires a comprehensive public health approach. This article focuses on evidence-based models of intervention to reduce violence while emphasizing collective impact as a guiding principle. Copyright © 2016 Elsevier Inc. All rights reserved.

  19. 2007 public transportation fact book

    Science.gov (United States)

    2007-05-01

    This book includes only public transportation data and excludes taxicab, unregulated jitney, school, sightseeing, intercity, charter, : military, and services not available to the general public, or segments of the general public (e.g., governmental ...

  20. Public Libraries: Adapting to Change.

    Science.gov (United States)

    Durrance, Joan; Van Fleet, Connie

    1992-01-01

    Reports on a study of changing trends in public libraries. Interviews with public library leaders identified five areas of changing emphasis: information technology, community needs, strategic planning, public relations and marketing, and funding. (seven references) (EA)

  1. Radiation risk and public education

    International Nuclear Information System (INIS)

    Faden, R.R.

    1983-01-01

    Two issues which deal with the public's perception of radiation hazards are discussed. The goal of public education about radiation, and the relative role of scientific and moral beliefs in public education are examined

  2. Public Undertakings and Imputability

    DEFF Research Database (Denmark)

    Ølykke, Grith Skovgaard

    2013-01-01

    In this article, the issue of impuability to the State of public undertakings’ decision-making is analysed and discussed in the context of the DSBFirst case. DSBFirst is owned by the independent public undertaking DSB and the private undertaking FirstGroup plc and won the contracts in the 2008...... Oeresund tender for the provision of passenger transport by railway. From the start, the services were provided at a loss, and in the end a part of DSBFirst was wound up. In order to frame the problems illustrated by this case, the jurisprudence-based imputability requirement in the definition of State aid...... in Article 107(1) TFEU is analysed. It is concluded that where the public undertaking transgresses the control system put in place by the State, conditions for imputability are not fulfilled, and it is argued that in the current state of law, there is no conditional link between the level of control...

  3. Taxation of library publications

    Directory of Open Access Journals (Sweden)

    Robert Razboršek

    2005-01-01

    Full Text Available The aim of this article is to stimulate the unified practice of the settlement of value added tax. This article draws from international and domestic law sources, which are fundamental for the taxation in Slovenia. As a rule, library publications are taxed with a 20% rate of value added tax, for imports into the European Union as well as within the European Union. The exception are printed publications which are, in the European Union, taxed with the lower, 8,5% rate and are tax exempt for imports from non-member countries of the European Union. The interlibrary loan is still entirely tax exempt,regulated as in the rest of Europe and abroad. If the purchases are made from other European Union countries or from countries outside the EU, from the perspective of the nacional economy, the so called self-taxation in the country receiving library publications is strongly supported.

  4. Public Participation GIS

    DEFF Research Database (Denmark)

    Hansen, Henning Sten

    2004-01-01

    The protection and enhancement of the environment is the main aim of most environmental planning, and the use of geographic information as well as public participation can improve the quality of both the processes and the decisions. The current paper describes the role of web-based geographic...... information in environmental planning and gives an overview over the various approaches to public participation. The current advances in Web-based GIS in many countries contain great possibilities for supporting good governance based on information and knowledge on the one hand and active involvement...... of the citizens on the other hand. One important precondition for success in this field is a well-informed population with access to the Internet. The overall purpose of this paper is to give en overview of how to utilise geographic information and public participation as natural components in environmental...

  5. Public Reason Renaturalized

    DEFF Research Database (Denmark)

    Tønder, Lars

    2014-01-01

    . The article develops this argument via a sensorial orientation to politics that not only re-frames existing critiques of neo-Kantianism but also includes an alternative, renaturalized conception of public reason, one that allows us to overcome the disconnect between the account we give of reason and the way......This article takes up recent discussions of nature and the sensorium in order to rethink public reason in deeply divided societies. The aim is not to reject the role of reason-giving but rather to infuse it with new meaning, bringing the reasonable back to its sensorially inflected circumstances...... it is mobilized in a world of deep pluralism. The article concludes with a discussion of how a renaturalized conception of public reason might change the positioning of contemporary democratic theory vis-a-vis the struggle for empowerment and pluralization in an age of neo-liberalism and state-surveillance....

  6. EIA publications directory, 1991

    International Nuclear Information System (INIS)

    1992-01-01

    Enacted in 1977, the Department of Energy (DOE) Organization Act established the Energy Information Administration (EIA) as the Department's independent statistical and analytical agency, with a mandate to collect and publish data and prepare analyses on energy production, consumption, prices, and resources, and projections of energy supply and demand. This edition of the EIA Publications Directory contains titles and abstracts of periodicals and one-time reports produced by the EIA from January through December 1991. This edition supplements EIA Publications Directory 1977--1989 and EIA Publications Directory 1990. The body of the Directory contains citations and abstracts arranged by broad subject categories, such as coal, petroleum, and natural gas and subcategories such as reserves, produces and byproducts, and marketing and economics. All reports are indexed alphabetically by subject and title and numerically by report number

  7. Audit of public work

    Directory of Open Access Journals (Sweden)

    Valtencir Godinho de Camargo

    2011-09-01

    Full Text Available The municipal public works, especially the reform and small constructions, with mobilization of manpower with up to 20 employees, where it is not mandatory elaboration of Program Conditions and Work Environment in the Construction Industry, are conducted without meeting the regulatory norm, which are motivated by the deficiency in the elaboration of the budget, the bidding documents, in the contract and supervision. The unfamiliarity specialized in the area of the Engineering of Safety of the Work adds to the factors that lead the public agency take unnecessary risks, as the objective responsibility, the labor charges and indemnities for illnesses, accidents or deaths. This article aims to indicate the guidelines for the program to health and safety is applied effectively in public works.

  8. Implementing public employment policy

    DEFF Research Database (Denmark)

    Larsen, Flemming; Bredgaard, Thomas

    disciplining of the unemployed (work first) (cf.Bredgaard & Larsen, 2005; Sol & Westerweld, 2005). It is, however, remarkable that in the research field there seems to be a division of labour so that changes in public administration and changes in the substance of employment policies are dealt with separately......Like most other areas within welfare policy, the employment and social policy areas are undergoing far-reaching changes in many countries. Partly in the shape of new forms of governance inspired by New Public Management (NPM), partly through new policies oriented towards activation and stronger....... But there is an interesting question to investigate here: whether and if so how, NPM-inspired reforms are related to changes in employment policy towards a work-first approach? Are changes in public management systems created as deliberate policy changes, or do they bring about more indirect and unintended policy changes...

  9. Offentlighed og Public Relations

    Directory of Open Access Journals (Sweden)

    Lars Heltoft

    1993-09-01

    Full Text Available Lars Heltoft, der er en af grundlæggerne af Public Relationsuddannelsen på Roskilde Universitetscenter, kaster i artiklen et kritisk blik på den akademiske forskning i feltet public relations. Selv i nyere udgaver af PR-forskningen - af mange anset for "progressive", f.eks. James Grunings og Jon Whites arbejder - ser Lars Heltoft tydelige spor af PR- professionens selvforståelse og legitimationsbehov. Resultatet er, at den "offentlighed", der burde være kernen i den videnskabelige beskæftigelse med public relations, "forsvinder i den blå luft". Han argumenterer for, at Habermas´ forestilling om "offentlighed" stadig institutionaliserer mulig- heden for kritisk diskurs inden for feltet, samtidig med at han i sproghand- lingsteoretisk perspektiv problematiserer Habermas´ legitimationsbegreb, fordi det undtager private virksomheder og organisationer.

  10. Keeping the "public" in schools of public health.

    Science.gov (United States)

    Freudenberg, Nicholas; Klitzman, Susan; Diamond, Catherine; El-Mohandes, Ayman

    2015-03-01

    In this article, we compared the characteristics of public and private accredited public health training programs. We analyzed the distinct opportunities and challenges that publicly funded schools of public health face in preparing the nation's public health workforce. Using our experience in creating a new, collaborative public school of public health in the nation's largest urban public university system, we described efforts to use our public status and mission to develop new approaches to educating a workforce that meets the health needs of our region and contributes to the goal of reducing health inequalities. Finally, we considered policies that could protect and strengthen the distinct contributions that public schools of public health make to improving population health and reducing health inequalities.

  11. Public awareness in Singapore

    International Nuclear Information System (INIS)

    Aziz Nather

    1999-01-01

    Public education is vital for the viability and development of any tissue bank. Whilst the Medical Therapy, Education and Research Act 1972 requiring consent from relative or next kin provides for procurement and transplantation of tissues, the public needs to be educated. Whilst much is known about kidney, liver and heart transplantation. NUH Tissue Bank adopts a 4-point strategy for public education:- 1.The Kidney Team headed by National Kidney Foundation has an aggressive, yearly public education drive. Our first strategy has been to join them in the National Kidney Donation Campaigns and slowly use their forum for Tissue Donation Drives. This has proved successful. 2. We took a key role in supporting the formation of the Society of transplantation of Singapore. I was elected Member of the Executive Committee and currently its Treasurer. The Society itself run scientific and public forums and with each one the public became more informed about us. 3.We ourselves actively publicise our workshops - seize every opportunity via TV, Radio, Newspaper, Hospital Bulletins, Campus News, Asian Medical News, etc. We did this once a year conservatively since 1995. 4. NUH Tissue Bank was invited to join a task force headed by Director of Medical Services, Dr Chen Ai Ju for increasing Organ and Tissue Donation in Singapore in January 1997. This has been very productive. Government is providing a one to two million dollars budget per year. March has been designed 'National Donation March'. The first drive will be in March 1999. In all our deliberation, we always take into consideration the cultural values and religious sensitivities of our multiracial population 80% Chinese, 15% Malays, 5% Indians

  12. Impact of ICRP publication 68

    International Nuclear Information System (INIS)

    Carter, M.W.; Woods, D.A.

    1996-01-01

    ICRP Publication 61 was a temporary replacement for ICRP Publication 30. It gave ALIs but not the underlying dose conversion factors. ICRP Publication 68 has now been issued to replace Publication 61; it contains the dose conversion factors but not the ALIs, so comparison is impossible without carrying out calculations. This paper presents comparisons between the two publications and calculates the ICRP Publication 68 ALIs for some of the more common radionuclides. (author)

  13. Public regulators and CSR

    DEFF Research Database (Denmark)

    Buhmann, Karin

    2016-01-01

    The social licence to operate (SLO) concept is little developed in the academic literature so far. Deployment of the term was made by the United National (UN) Guiding Principles on Business and Human Rights and the UN ‘Protect, Respect and Remedy’ Framework, which apply SLO as an argument...... for responsible business conduct, connecting to social expectations and bridging to public regulation. This UN guidance has had a significant bearing on how public regulators seek to influence business conduct beyond Human Rights to broader Corporate Social Responsibility (CSR) concerns. Drawing on examples...

  14. Tracing Public Values Change

    DEFF Research Database (Denmark)

    Beck Jørgensen, Torben; Rutgers, Mark R.

    2014-01-01

    Long term changes in public values are not easily detected. One important reason is the limited availability of reliable empirical data. Job advertisements allow us to go back in history for some decades and job ads may present us with the values that are supposed to guide civil servant behaviour...... in several directions; b) job ads develop into platforms for organizational branding with an emphasis on HR-related values although national logos enter the scene (the Danish royal crown, the Dutch national emblem); c) New Public Management values do not crowd out other values, rather value intensity...

  15. Representatives of the Public?

    DEFF Research Database (Denmark)

    Rasmussen, Anne; Carroll, Brendan; Lowery, David

    2014-01-01

    Although scholars have long speculated about how organised interests link the public to decision makers, there has actually been little empirical research on this important element of democratic theory. This important gap in the literature is addressed in this article by examining, in addition...... to other supply-side and demand-side factors, whether groups mobilise on issues in policy areas that are regarded as salient by the public. Based on an analysis of 4,501 contributions in 142 European Commission online consultations, it is found that organised interests potentially can act as a transmission...

  16. Publications, 1977 through 1980

    International Nuclear Information System (INIS)

    Hilborn, H.S.

    1981-04-01

    This is a compilation of documents that communicate the results of scientific and technical work done at Savannah River. The compilation includes those documents that have been published (research and development reports, journal articles, book chapters, etc.), and documents that have been announced in Energy Research Abstracts. Where applicable the meeting at which the paper was presented is given. The information was compiled by machine methods to produce bibliographic, subject, and author listings. This report updates the information included in DP-929, Rev. 2, Publications, 1951 through 1971 and DP-929-1, Publications, 1972 through 1976, and replaces DP-929-1, Supplement 3

  17. Database Publication Practices

    DEFF Research Database (Denmark)

    Bernstein, P.A.; DeWitt, D.; Heuer, A.

    2005-01-01

    There has been a growing interest in improving the publication processes for database research papers. This panel reports on recent changes in those processes and presents an initial cut at historical data for the VLDB Journal and ACM Transactions on Database Systems.......There has been a growing interest in improving the publication processes for database research papers. This panel reports on recent changes in those processes and presents an initial cut at historical data for the VLDB Journal and ACM Transactions on Database Systems....

  18. Nuclear public relations campaign

    Energy Technology Data Exchange (ETDEWEB)

    1983-01-01

    A two-day hearing to investigate DOE's participation in a $30 million nuclear public relations program at a time when the country is asked to decrease spending and in conjunction with similar efforts by the nuclear industry focused on the issue of whether ratepayers or shareholders should finance the effort. Shelby Brewer, Assistant Secretary for Nuclear Energy, defended the expenditures as a response to public demand for information and the need to correct misinformation. The testimony of 14 other witnesses includes the views of citizen and professional groups, utilities, and government agencies. Their testimony is followed by additional material submitted for the record. (DCK)

  19. Transforming Public Space

    DEFF Research Database (Denmark)

    Navarro, Dora

    2009-01-01

    Among processes towards democratisation, it has been asserted that alternative radio has a central role in the citizen making of the poor. However, it is important to analyse in detail what possibilities an alternative or citizens' radio has to strengthen ideas of citizenship and transform...... the public space into a critical and deliberative public in urban sites. I focus on one local Catholic radio station in Huaycan, a shantytown in the outskirts of Lima, Peru. I describe the radios' journalistic work, showing examples of how they mobilise local leaders and monitor democratic processes...

  20. Adventures in public data.

    Science.gov (United States)

    Zaharevitz, Dan W

    2011-10-14

    This article contains the slides and transcript of a talk given by Dan Zaharevitz at the "Visions of a Semantic Molecular Future" symposium held at the University of Cambridge Department of Chemistry on 2011-01-19. A recording of the talk is available on the University Computing Service's Streaming Media Service archive at http://sms.cam.ac.uk/media/1095515 (unfortunately the first part of the recording was corrupted, so the talk appears to begin at slide 6, 'At a critical time'). We believe that Dan's message comes over extremely well in the textual transcript and that it would be poorer for serious editing. In addition we have added some explanations and references of some of the concepts in the slides and text. (Charlotte Bolton; Peter Murray-Rust, University of Cambridge) EDITORIAL PREFACE: The following paper is part of a series of publications which arose from a Symposium held at the Unilever Centre for Molecular Informatics at the University of Cambridge to celebrate the lifetime achievements of Peter Murray-Rust. One of the motives of Peter's work was and is a better transport and preservation of data and information in scientific publications. In both respects the following publication is relevant: it is about public data and their representation, and the publication represents a non-standard experiment of transporting the content of the scientific presentation. As you will see, it consists of the original slides used by Dan Zaharevitz in his talk "Adventures in Public Data" at the Unilever Centre together with a diligent transcript of his speech. The transcribers have gone through great effort to preserve the original spirit of the talk by preserving colloquial language as it is used at such occasions. For reasons known to us, the original speaker was unable to submit the manuscript in a more conventional form. We, the Editors, have discussed in depth whether such a format is suitable for a scientific journal. We have eventually decided to publish this

  1. Adventures in public data

    Directory of Open Access Journals (Sweden)

    Zaharevitz Dan W

    2011-10-01

    Full Text Available Abstract This article contains the slides and transcript of a talk given by Dan Zaharevitz at the "Visions of a Semantic Molecular Future" symposium held at the University of Cambridge Department of Chemistry on 2011-01-19. A recording of the talk is available on the University Computing Service's Streaming Media Service archive at http://sms.cam.ac.uk/media/1095515 (unfortunately the first part of the recording was corrupted, so the talk appears to begin at slide 6, 'At a critical time'. We believe that Dan's message comes over extremely well in the textual transcript and that it would be poorer for serious editing. In addition we have added some explanations and references of some of the concepts in the slides and text. (Charlotte Bolton; Peter Murray-Rust, University of Cambridge Editorial preface The following paper is part of a series of publications which arose from a Symposium held at the Unilever Centre for Molecular Informatics at the University of Cambridge to celebrate the lifetime achievements of Peter Murray-Rust. One of the motives of Peter's work was and is a better transport and preservation of data and information in scientific publications. In both respects the following publication is relevant: it is about public data and their representation, and the publication represents a non-standard experiment of transporting the content of the scientific presentation. As you will see, it consists of the original slides used by Dan Zaharevitz in his talk "Adventures in Public Data" at the Unilever Centre together with a diligent transcript of his speech. The transcribers have gone through great effort to preserve the original spirit of the talk by preserving colloquial language as it is used at such occasions. For reasons known to us, the original speaker was unable to submit the manuscript in a more conventional form. We, the Editors, have discussed in depth whether such a format is suitable for a scientific journal. We have eventually

  2. Experts on public trial

    DEFF Research Database (Denmark)

    Blok, Anders

    2007-01-01

    a case study of the May 2003 Danish consensus conference on environmental economics as a policy tool, the article reflects on the politics of expert authority permeating practices of public participation. Adopting concepts from the sociology of scientific knowledge (SSK), the conference is seen......-than-successful defense in the citizen perspective. Further, consensus conferences are viewed alternatively as "expert dissent conferences," serving to disclose a multiplicity of expert commitments. From this perspective, some challenges for democratizing expertise through future exercises in public participation...

  3. Public and popular history

    CERN Document Server

    De Groot, Jerome

    2013-01-01

    This interdisciplinary collection considers public and popular history within a global framework, seeking to understand considerations of local, domestic histories and the ways they interact with broader discourses. Grounded in particular local and national situations, the book addresses the issues associated with popular history in a globalised cultural world, such as: how the study of popular history might work in the future; new ways in which the terms 'popular' and 'public' might inform one another and nuance scholarship; transnational, intercultural models of 'pastness'; cultural translat

  4. Public relations strategy

    International Nuclear Information System (INIS)

    Kukovica, T.; Mele, I.

    1996-01-01

    NIMBY (Not In My Back Yard) syndrome has become a major problem in the field of environmental planning. Also in Slovenia. Agency for Radwaste management was established by the Slovene government in 1991. Our main objective is to provide the final disposal of radioactive waste in Slovenia. Overcoming the NIMBY syndrome is therefore the primary aim of Agency's PR (public relations) strategy. Agency's public relations strategy was prepared in 1993. After very unpleasant experience with NIMBY syndrome in local community where one of the potential locations for low and intermediate level repository was identified the strategy was renewed in 1994. It is divided into two parts: information and education. The first one includes providing information to the media, surveys of press clippings, interviews, press conferences, public opinion polls and publishing articles. Agency's first task in the field of education was to prepare different materials like: leaflets, Agency's newspaper and videos. Materials were distributed to various target publics. There is also a special program of lectures for youngsters. All those activities and projects have been successfully realized within the last year. Plans of PR activities for this year are focused on informing the local community about remediation of temporary storage of radioactive waste near village Zavratec. Other new projects are also under preparation like: new leaflets, presentation on Internet, journalist's guide to rad waste management, most frequently asked questions and answers. (author)

  5. Nuclear power publications

    International Nuclear Information System (INIS)

    1982-01-01

    This booklet lists 69 publications on nuclear energy available free from some of the main organisations concerned with its development and operation in the UK. Headings are: general information; the need for nuclear energy; the nuclear industry; nuclear power stations; fuel cycle; safety; waste management. (U.K.)

  6. Public management and governance

    National Research Council Canada - National Science Library

    Bovaird, A. G; Löffler, Elke

    2009-01-01

    ... how the process of governing needs to be fundamentally altered if a government is to retain public trust and make better use of society's resources. Key themes covered include: ■ ■ ■ ■ the challenges and pressures which governments experience in an international context; the changing functions of modern government in the global economy; the 'mixed ec...

  7. Voluntary Public Unemployment Insurance

    DEFF Research Database (Denmark)

    O. Parsons, Donald; Tranæs, Torben; Bie Lilleør, Helene

    Denmark has drawn much attention for its active labor market policies, but is almost unique in offering a voluntary public unemployment insurance program requiring a significant premium payment. A safety net program – a less generous, means-tested social assistance plan – completes the system...

  8. Issues in public health

    National Research Council Canada - National Science Library

    Sim, Fiona; McKee, Martin

    2011-01-01

    ..., there is increasing understanding of the inevitable limits of individual health care and of the need to complement such services with effective public health strategies. Major improvements in people's health will come from controlling communicable diseases, eradicating environmental hazards, improving people's diets and enhancing the availability ...

  9. A Publications Sampler.

    Science.gov (United States)

    Hess, Sheila

    1984-01-01

    Lists over 100 association publications on topics of: aeronautics and space, aging, arts and architecture, computers, consumer guides, education, educational directories, government and politics, handicapped, health and medicine, housing and land use, libraries, management, recreation and hobbies, science and technology, social issues. A list of…

  10. Coal and public perceptions

    International Nuclear Information System (INIS)

    Porter, R.C.

    1993-01-01

    The Department of Energy's (DOE) clean coal outreach efforts are described. The reason why clean coal technology outreach must be an integral part of coal's future is discussed. It is important that we understand the significance of these advances in coal utilization not just in terms of of hardware but in terms of public perception. Four basic premises in the use of coal are presented. These are: (1) that coal is fundamentally important to this nation's future; (2) that, despite premise number 1, coal's future is by no means assured and that for the last 10 years, coal has been losing ground; (3) that coal's future hinges on the public understanding of the benefits of the public's acceptance of advanced clean coal technology; and (4) hat public acceptance of clean coal technology is not going to be achieved through a nationwide advertising program run by the Federal government or even by the private sector. It is going to be gained at the grassroots level one community at a time, one plant at a time, and one referendum at a time. The Federal government has neither the resources, the staff, nor the mandate to lead the charge in those debates. What is important is that the private sector step up to the plate as individual companies and an individual citizens working one-one-one at the community level, one customer, one civic club, and one town meeting at a time

  11. Science, Public Engagement with

    DEFF Research Database (Denmark)

    Irwin, Alan

    2015-01-01

    regarding their definition in institutional practice. Science and technology studies scholars have been especially active in challenging prevailing policy assumptions in this area and in considering how science–public relations might be reinterpreted and reconstructed. This article presents some of the key...

  12. Publications about Radon

    Science.gov (United States)

    There is no known safe level of exposure to radon. EPA strongly recommends that you fix your home if your test shows 4 picocuries (pCi/L) or more. These publications and resources will provide you with the information you need to fix your home.

  13. Small public private partnerships

    DEFF Research Database (Denmark)

    Koch, Christian; Jensen, Jesper Ole

    2009-01-01

    Public Private Partnerships (PPP) are frequently mobilized as a purchasing form suitable for large infrastructure projects. And it is commonly assumed that transaction costs linked to the establishment of PPP make them prohibitive in small sizes. In a Danish context this has been safeguarded by t...

  14. Teaching Mediated Public Relations.

    Science.gov (United States)

    Kent, Michael L.

    2001-01-01

    Discusses approaches to teaching a mediated public relations course, emphasizing the World Wide Web. Outlines five course objectives, assignments and activities, evaluation, texts, and lecture topics. Argues that students mastering these course objectives will understand ethical issues relating to media use, using mediated technology in public…

  15. Public mental health.

    Science.gov (United States)

    Lindert, Jutta; Bilsen, Johan; Jakubauskiene, Marija

    2017-10-01

    Public mental health (PMH) is a major challenge for public health research and practice. This article is organized in six parts. First, we will highlight the significance of PMH; second, we will define mental health and mental disorders; third, we identify and describe determinants of mental health and mental disorders on which we worked in the past 10 years since the establishment of the PMH section such as social determinants and violence. Fourth, we will describe the development of the EUPHA PMH section and provide details on vulnerable groups in the field of PMH, on violence as a main determinant and on suicide as an outcome which affects all countries in the European region. Fifth, we describe policy and practice implications of the development of PMH and highlight the European dimension of PMH. We will conclude this article by providing an outlook on potential further development of PMH as regards research and policy and practice. Finally, we hope that the EUPHA PMH section will contribute to public health in the next 25 years and we can contribute to improvement of PMH in Europe. © The Author 2017. Published by Oxford University Press on behalf of the European Public Health Association. All rights reserved.

  16. Public Speaking: Revisited.

    Science.gov (United States)

    Taufen, Phyllis M.

    There is a simple but effective process for developing public speakers in elementary and junior high schools. After discussing the importance of effective speaking, the teacher puts a topic sentence, on favorite desserts for example, on the board or overhead projector and students think of their favorite desserts and some related words and…

  17. Bibliography of publications, 1990

    International Nuclear Information System (INIS)

    1992-01-01

    This issue is the bibliography of the publication by the staffs of the Radiation Effects Research Institute (RERF) in 1990. Titles in original and English language with author names are enumerated. Indexes by research areas and by author name are also attached. (J.P.N.) 126 refs

  18. Public Service Telecommunications: PEACESAT.

    Science.gov (United States)

    Okamura, Norman H.; Mukaida, Lori Van Dusen

    1996-01-01

    Describes the Pan-Pacific Education and Communication Experiments by Satellite (PEACESAT), a public service telecommunication program developed to facilitate the use of telecommunications and information technologies. Highlights include the historical development; digital capabilities; cooperation and collaboration with users, government, and…

  19. A Public Secret

    DEFF Research Database (Denmark)

    Bregnbæk, Susanne

    2011-01-01

    This article is based on anthropological fieldwork undertaken at two elite universities in Beijing. It addresses the paradoxical situation of the many instances of suicide among Chinese elite university students in Beijing, which constitute a public secret. The pressure of education weighs heavily...

  20. Publicity and Egalitarian Justice

    DEFF Research Database (Denmark)

    Lippert-Rasmussen, Kasper

    2008-01-01

    Recently, the issue of publicity has surfaced in discussions of the correct interpretation of the Rawlsian principles of justice. In an intriguing critique of G.A. Cohen's preferred interpretation of the difference principle as a principle that is incompatible with incentive-based inequalities...

  1. Public Enterprises in Ethiopia

    African Journals Online (AJOL)

    Tewodros_M

    varies depending on the political economy or ideology of a state. It is common to find ... continues discussing the theme in the Ethiopian context. 1. Defining the term ... economic decision making and statistical data on the size of the public sector with a view to analyzing economic implications and other issues. The same.

  2. Public Libraries in Bangladesh.

    Science.gov (United States)

    Khan, M. H.

    1984-01-01

    Overview of library movement in Bangladesh highlights British (1851-1947) and Pakistan periods (1947-1971), separation of Bangladesh from Pakistan, libraries in development plans (1951-1970), three important public libraries, development of national library, book resources, a library network plan, legislation, finance, leadership, library…

  3. Public inquiries in Canada

    International Nuclear Information System (INIS)

    Bourque, D.

    1992-01-01

    The Canadian Constitution provides that the provinces may exclusively make laws in relation to electrical generation facilities. However through the exercise of its declaratory power, which is an exceptional power assigned to the federal Parliament, the latter has assumed exclusive legislative jurisdiction over the nuclear fuel cycle. Thus, the legislation applying to nuclear power plants is that which is passed by the federal Parliament. On June 18, 1990, the Canadian government tabled Bill C-78 (Environmental Assessment Act), which clarifies the nature, purpose and scope of public hearings into the construction of nuclear power plants. These reflections upon the role of the public hearing in the Canadian context lead to more general observations on the need to restructure decision-making mechanisms in order to take into account new values that important segments of the population are espousing. More open and democratic decision-making mechanisms, both for developing the content of energy policies and reviewing the construction of specific projects, constitute one of the avenues that should be explored in order to arrive at better public understanding and acceptance of nuclear energy. Public hearings can play an important role in this restructuring of decision-making mechanisms. Precise rules must nevertheless be established in order to use this means effectively and to avoid raising false expectations. (author)

  4. Rally as a Political Public Relations Strategy for Public Acceptance ...

    African Journals Online (AJOL)

    This study examines the assessment of the use of rally as a political public relations strategy for public acceptance of a political party during the 2015 presidential elections in Lagos State. Public relations uses tactical methods of communication to build relations between an organisation and its internal and external publics.

  5. THE PUBLIC ADMINISTRATION COMPARED TO PUBLIC MANAGEMENT AND OTHER SCIENCES

    Directory of Open Access Journals (Sweden)

    LAZAR IOAN

    2010-12-01

    Full Text Available In this paper we try to analyze the meaning of the public administration term. In our opinion it is necessary to be able to define exactly what public administration means but at the same time to clearly distinguish it from the other sciences. This is due to the fact that very often confusion is made between public administration and public management, public administration and bureaucracy or public administration and policy. Throughout this paper we hope to clarify some of the aspects related to what exactly distinguishes public administration from the above mentioned sciences and what are its own particularities.

  6. Communicating with external publics: managing public opinion and behavior.

    Science.gov (United States)

    Ristino, Robert J

    2007-01-01

    In health care organizational settings public relations plays an important role in managing relationships with a variety of external publics as well as with society in general. Managing these relationships involves both reactive and proactive communication activities. Reactively, public relations responds to public issues, crises and concerns, as well as inquiries from the media and other social institutions. Proactively, public relations engages in deliberately planned campaigns and programs to inform, influence or change behaviors of targeted publics for a wide range of strategic purposes. These purposes include managing the organization's image and identity; influencing public policies; supporting health promotion and education; promoting fund raising and volunteerism; and managing organizational change and crises.

  7. [The treatment received by public health services users in Mexico].

    Science.gov (United States)

    Puentes Rosas, Esteban; Gómez Dantés, Octavio; Garrido Latorre, Francisco

    2006-06-01

    To document the fact that differences in the treatment received by health services users in Mexico are mainly dependent on the type of provider, regardless of the users' socioeconomic status. The data were obtained by means of a survey of 18 018 users who visited 73 health services in 13 states within Mexico. They were asked to grade the way the institution had performed in seven of the eight domains that define appropriate user treatment (autonomy, confidentiality, communication, respectful manner, condition of basic facilities, access to social assistance networks, and free user choice). The questionnaire included some vignettes to help determine user expectations. A composite ordinal probit model was applied; the perception of quality in connection with each of the appropriate treatment domains was the independent variable, whereas gender, educational level, age, type of provider, and user expectations were used as control variables. The type of provider was the main factor that determined users' perceptions regarding the treatment they received when visiting health services in Mexico. Institutions belonging to the social security system performed the worst, while the services provided under the program targeting the rural population (IMSS Oportunidades) received the highest scores. Overall, the domain that was most highly ranked was respectful manner, whereas the lowest score was given to the ability to choose the provider. Men felt they had been able to communicate better than women, while respectful manner, communication, and social support showed a significant negative association with educational level (P < 0.05). Differences were noted in the way different public health service providers in Mexico treat their users, regardless of the latter's socioeconomic status. Social security system providers showed the greatest deficiencies in this respect. Respectful manner was the domain that received the highest scores in the case of all providers. Organizational

  8. Intravenous infusion of docosahexaenoic acid increases serum concentrations in a dose-dependent manner and increases seizure latency in the maximal PTZ model.

    Science.gov (United States)

    Trépanier, Marc-Olivier; Kwong, Kei-Man; Domenichiello, Anthony F; Chen, Chuck T; Bazinet, Richard P; Burnham, W M

    2015-09-01

    Docosahexaenoic acid (DHA) is an omega-3 polyunsaturated fatty acid (n-3 PUFA) that has been shown to raise seizure thresholds in the maximal pentylenetetrazole model following acute subcutaneous (s.c.) administration in rats. Following s.c. administration, however, the dose-response relationship for DHA has shown an inverted U-pattern. The purposes of the present experiment were as follows: (1) to determine the pattern of serum unesterified concentrations resulting from the intravenous (i.v.) infusions of various doses of DHA, (2) to determine the time course of these concentrations following the discontinuation of the infusions, and (3) to determine whether seizure protection in the maximal PTZ model would correlate with serum unesterified DHA levels. Animals received 5-minute i.v. infusions of saline or 25, 50, 100, or 200mg/kg of DHA via a cannula inserted into one of the tail veins. Blood was collected during and after the infusions by means of a second cannula inserted into the other tail vein (Experiment 1). A separate group of animals received saline or 12.5-, 25-, 50-, 100-, or 200 mg/kg DHA i.v. via a cannula inserted into one of the tail veins and were then seizure-tested in the maximal PTZ model either during infusion or after the discontinuation of the infusions. Slow infusions of DHA increased serum unesterified DHA concentrations in a dose-dependent manner, with the 200-mg/kg dose increasing the concentration approximately 260-fold compared with saline-infused animals. Following discontinuation of the infusions, serum concentrations rapidly dropped toward baseline, with half-lives of approximately 40 and 11s for the 25-mg/kg dose and 100-mg/kg dose, respectively. In the seizure-tested animals, DHA significantly increased latency to seizure onset in a dose-dependent manner. Following the discontinuation of infusion, seizure latency rapidly decreased toward baseline. Overall, our study suggests that i.v. infusion of unesterified DHA results in

  9. Cisplatin-resistant lung cancer cell–derived exosomes increase cisplatin resistance of recipient cells in exosomal miR-100–5p-dependent manner

    Directory of Open Access Journals (Sweden)

    Qin X

    2017-05-01

    Full Text Available Xiaobing Qin,1,2,* Shaorong Yu,1,3,* Leilei Zhou,1,4 Meiqi Shi,3 Yong Hu,1 Xiaoyue Xu,1 Bo Shen,1 Siwen Liu,1 Dali Yan,1 Jifeng Feng1,3 1Research Center for Clinical Oncology, Affiliated Cancer Hospital of Nanjing Medical University, Jiangsu Cancer Hospital and Jiangsu Institute of Cancer Research, Nanjing, 2Department of Oncology, Xuzhou First People’s Hospital, Xuzhou, 3Department of Oncology, Affiliated Cancer Hospital of Nanjing Medical University, Jiangsu Cancer Hospital and Jiangsu Institute of Cancer Research, Nanjing, 4Department of Oncology, Affiliated Huai’an Hospital of Nanjing Medical University, Huai’an, Jiangsu, China *These authors have contributed equally to this work Abstract: Exosomes derived from lung cancer cells confer cisplatin (DDP resistance to other cancer cells. However, the underlying mechanism is still unknown. A549 resistance to DDP (A549/DDP was established. Microarray was used to analyze microRNA (miRNA expression profiles of A549 cells, A549/DDP cells, A549 exosomes, and A549/DDP exosomes. There was a strong correlation of miRNA profiles between exosomes and their maternal cells. A total of 11 miRNAs were significantly upregulated both in A549/DDP cells compared with A549 cells and in exosomes derived from A549/DDP cells in contrast to exosomes from A549 cells. A total of 31 downregulated miRNAs were also observed. miR-100–5p was the most prominent decreased miRNA in DDP-resistant exosomes compared with the corresponding sensitive ones. Downregulated miR-100–5p was proved to be involved in DDP resistance in A549 cells, and mammalian target of rapamycin (mTOR expression was reverse regulated by miR-100–5p. Exosomes confer recipient cells’ resistance to DDP in an exosomal miR-100–5p-dependent manner with mTOR as its potential target both in vitro and in vivo. Exosomes from DDP-resistant lung cancer cells A549 can alter other lung cancer cells’ sensitivity to DDP in exosomal miR-100–5p

  10. Budget Planning and the Quality of Educational Services in Uganda Public Universities: A Case Study of Kyambogo University

    Science.gov (United States)

    Basheka, Benon C.; Nabwire, Addah

    2013-01-01

    This paper examines the relationship between budget planning and the quality of educational services at Kyambogo University in Uganda. We argue that the manner in which the university's budget planning activities are conducted determines in a significant way (by 76.8%) the quality of the services offered by public universities in Uganda. The…

  11. How Public Opinion is Formed

    Science.gov (United States)

    Block, Edward M.

    1977-01-01

    Investigates the evolution of the definition of public relations by examining cultural and personal determinants of public opinion. Outlines functions of communicators and opinionmakers in forming and influencing public opinion. Available from: Public Relations Review, Ray Hiebert, Dean, College of Journalism, University of Maryland, College Park,…

  12. Perceptions of Public Relations Education.

    Science.gov (United States)

    Stacks, Don W.; Botan, Carl; Turk, Judy VanSlyke

    1999-01-01

    Surveys 258 public-relations educators and practitioners, finding they agree that public-relations education is on track; that systematic assessment is an important feature of public-relations education; and that they agreed on how public-relations education should be structured, and demonstrated a high degree of similarity in their preferences…

  13. Are public opinion polls self-fulfilling prophecies?

    Directory of Open Access Journals (Sweden)

    David Rothschild

    2014-08-01

    Full Text Available Psychologists have long observed that people conform to majority opinion, a phenomenon sometimes referred to as the ‘bandwagon effect’. In the political domain people learn about prevailing public opinion via ubiquitous polls, which may produce a bandwagon effect. Newer types of information – published probabilities derived from prediction market contract prices and aggregated polling summaries – may have similar effects. Consequently, polls can become self-fulfilling prophecies whereby majorities, whether in support of candidates or policies, grow in a cascading manner. Despite increased attention to whether the measurement of public opinion can itself affect public opinion, the existing empirical literature is surprisingly limited on the bandwagon effects of polls. To address this gap, we conducted an experiment on a diverse national sample in which we randomly assigned people to receive information about different levels of support for three public policies. We find that public opinion as expressed through polls affects individual-level attitudes, although the size of the effect depends on issue characteristics.

  14. THE DECISION OF GOING PUBLIC – WHEN, WHERE AND WHY?

    Directory of Open Access Journals (Sweden)

    PĂTRAȘCU (SANDU, DIANA – RAMONA

    2014-02-01

    Full Text Available The decisions of the company, in what it concerns financial resources, play a key role in its existence and evolution. Funding through the stock exchange, due to its implications, is especially challenging in the current economic and financial enviroment. By bringing into question the results of relevant studies, we aimed to answer three key questions about the decision of firms to become public: when is the appropriate time, on what market to start selling primary shares and wich are the main implications of this decision? Although a theoretical work, we consider the paper to be of interest due to the whay results of specialized studies were systematized. The overall conclusion is that there are no firm or unanimous answers. Most opinions converge to the idea that the perfect time for a company to become public is when the stock market is growing or marked by optimism. Regarding the question on what market to open to the public investors, most studies recommend international markets due to their size and higher chances of success. However, as any financial decision, the decision to become public involves positive and negative effects. Arguments in favor of the initiative to become public and cons of such an approach are addressed in the same manner, by reference to relevant studies undertaken in the field.

  15. High Gestational Folic Acid Supplementation Alters Expression of Imprinted and Candidate Autism Susceptibility Genes in a sex-Specific Manner in Mouse Offspring.

    Science.gov (United States)

    Barua, Subit; Kuizon, Salomon; Brown, W Ted; Junaid, Mohammed A

    2016-02-01

    Maternal nutrients play critical roles in modulating epigenetic events and exert long-term influences on the progeny's health. Folic acid (FA) supplementation during pregnancy has decreased the incidence of neural tube defects in newborns, but the influence of high doses of maternal FA supplementation on infants' brain development is unclear. The present study was aimed at investigating the effects of a high dose of gestational FA on the expression of genes in the cerebral hemispheres (CHs) of 1-day-old pups. One week prior to mating and throughout the entire period of gestation, female C57BL/6J mice were fed a diet, containing FA at either 2 mg/kg (control diet (CD)) or 20 mg/kg (high maternal folic acid (HMFA)). At postnatal day 1, pups from different dams were sacrificed and CH tissues were collected. Quantitative RT-PCR and Western blot analysis confirmed sex-specific alterations in the expression of several genes that modulate various cellular functions (P < 0.05) in pups from the HMFA group. Genomic DNA methylation analysis showed no difference in the level of overall methylation in pups from the HMFA group. These findings demonstrate that HMFA supplementation alters offsprings' CH gene expression in a sex-specific manner. These changes may influence infants' brain development.

  16. Mineral and Skeletal Homeostasis Influence the Manner of Bone Loss in Metabolic Osteoporosis due to Calcium-Deprived Diet in Different Sites of Rat Vertebra and Femur

    Directory of Open Access Journals (Sweden)

    Marzia Ferretti

    2015-01-01

    Full Text Available Rats fed calcium-deprived diet develop osteoporosis due to enhanced bone resorption, secondary to parathyroid overactivity resulting from nutritional hypocalcemia. Therefore, rats provide a good experimental animal model for studying bone modelling alterations during biochemical osteoporosis. Three-month-old Sprague-Dawley male rats were divided into 4 groups: (1 baseline, (2 normal diet for 4 weeks, (3 calcium-deprived diet for 4 weeks, and (4 calcium-deprived diet for 4 weeks and concomitant administration of PTH (1-34 40 µg/Kg/day. Histomorphometrical analyses were made on cortical and trabecular bone of lumbar vertebral body as well as of mid-diaphysis and distal metaphysis of femur. In all rats fed calcium-deprived diet, despite the reduction of trabecular number (due to the maintenance of mineral homeostasis, an intense activity of bone deposition occurs on the surface of the few remaining trabeculae (in answering to mechanical stresses and, consequently, to maintain the skeletal homeostasis. Different responses were detected in different sites of cortical bone, depending on their main function in answering mineral or skeletal homeostasis. This study represents the starting point for work-in-progress researches, with the aim of defining in detail timing and manners of evolution and recovery of biochemical osteoporosis.

  17. How Age, Linguistic Status, and the Nature of the Auditory Scene Alter the Manner in Which Listening Comprehension Is Achieved in Multitalker Conversations.

    Science.gov (United States)

    Avivi-Reich, Meital; Jakubczyk, Agnes; Daneman, Meredyth; Schneider, Bruce A

    2015-10-01

    We investigated how age and linguistic status affected listeners' ability to follow and comprehend 3-talker conversations, and the extent to which individual differences in language proficiency predict speech comprehension under difficult listening conditions. Younger and older L1s as well as young L2s listened to 3-talker conversations, with or without spatial separation between talkers, in either quiet or against moderate or high 12-talker babble background, and were asked to answer questions regarding their contents. After compensating for individual differences in speech recognition, no significant differences in conversation comprehension were found among the groups. As expected, conversation comprehension decreased as babble level increased. Individual differences in reading comprehension skill contributed positively to performance in younger EL1s and in young EL2s to a lesser degree but not in older EL1s. Vocabulary knowledge was significantly and positively related to performance only at the intermediate babble level. The results indicate that the manner in which spoken language comprehension is achieved is modulated by the listeners' age and linguistic status.

  18. Nogo-B Promotes Angiogenesis in Proliferative Diabetic Retinopathy via VEGF/PI3K/Akt Pathway in an Autocrine Manner

    Directory of Open Access Journals (Sweden)

    Yuelu Zhang

    2017-10-01

    Full Text Available Background/Aims: Nogo-B, a conservative protein of endoplasmic reticulum, is a member of the reticulon family of proteins. Proliferative diabetic retinopathy (PDR is the major concerning problem of diabetic retinopathy. This study explored the role of Nogo-B in the regulation of angiogenesis in PDR patients and primary human retinal endothelial cells (HRMECs. Methods: Nogo-B was down-regulated through the use of Lentivirus-NogoB-RNAi, the effects of Nogo-B on angiogenesis under high glucose stimulation were evaluated via CCK-8 assay, wound closure assay, transwell assay, and tube formation assay. Expression of Nogo-B, VEGF, PI3K and Akt were determined by western blotting, immunofluorescence, enzyme-linked immunosorbent assay (ELISA. Co-culture systerm was used to explore cell communication. Results: Nogo-B was highly enriched in ocular tissues of PDR patients and in HRMECs exposed to high glucose. Down-regulation of Nogo-B attenuated high glucose induced cell migration and tube formation in HRMECs. Mechanistically, in comparison with the negative control group, Lentivirus-NogoB-RNAi group had exhibited reduced VEGF secretion, weakened PI3K and Akt activation. Besides, high glucose treatment promoted the secretion of Nogo-B and presented as a “long-term memory”. Conclusions: These data collectively indicated that Nogo-B promoted angiogenesis in HRMECs via VEGF/PI3K/Akt pathway in an autocrine manner.

  19. Gpn3 is polyubiquitinated on lysine 216 and degraded by the proteasome in the cell nucleus in a Gpn1-inhibitable manner.

    Science.gov (United States)

    Méndez-Hernández, Lucía E; Robledo-Rivera, Angelica Y; Macías-Silva, Marina; Calera, Mónica R; Sánchez-Olea, Roberto

    2017-11-01

    Gpn1 associates with Gpn3, and both are required for RNA polymerase II nuclear targeting. Global studies have identified by mass spectrometry that human Gpn3 is ubiquitinated on lysines 189 and 216. Our goals here were to determine the type, physiological importance, and regulation of Gpn3 ubiquitination. After inhibiting the proteasome with MG132, Gpn3-Flag was polyubiquitinated on K216, but not K189, in HEK293T cells. Gpn3-Flag exhibited nucleo-cytoplasmic shuttling, but polyubiquitination and proteasomal degradation of Gpn3-Flag occurred only in the cell nucleus. Polyubiquitination-deficient Gpn3-Flag K216R displayed a longer half-life than Gpn3-Flag in two cell lines. Interestingly, Gpn1-EYFP inhibited Gpn3-Flag polyubiquitination in a dose-dependent manner. In conclusion, Gpn1-inhibitable, nuclear polyubiquitination on lysine 216 regulates the half-life of Gpn3 by tagging it for proteasomal degradation. © 2017 Federation of European Biochemical Societies.

  20. Ptpn11 Deletion in CD4+ Cells Does Not Affect T Cell Development and Functions but Causes Cartilage Tumors in a T Cell-Independent Manner.

    Science.gov (United States)

    Miah, S M Shahjahan; Jayasuriya, Chathuraka T; Salter, Alexander I; Reilly, Emma C; Fugere, Céline; Yang, Wentian; Chen, Qian; Brossay, Laurent

    2017-01-01

    The ubiquitously expressed tyrosine phosphatase Src homology region 2 domain-containing phosphatase-2 (SHP-2, encoded by Ptpn11 ) is required for constitutive cellular processes including proliferation, differentiation, and the regulation of immune responses. During development and maturation, subsets of T cells express a variety of inhibitory receptors known to associate with phosphatases, which in turn, dephosphorylate key players of activating receptor signaling pathways. We hypothesized that SHP-2 deletion would have major effects on T cell development by altering the thresholds for activation, as well as positive and negative selection. Surprisingly, using mice conditionally deficient for SHP-2 in the T cell lineage, we show that the development of these lymphocytes is globally intact. In addition, our data demonstrate that SHP-2 absence does not compromise T cell effector functions, suggesting that SHP-2 is dispensable in these cells. Unexpectedly, in aging mice, Ptpn11 gene deletion driven by CD4 Cre recombinase leads to cartilage tumors in wrist bones in a T cell-independent manner. These tumors resemble miniature cartilaginous growth plates and contain CD4-lineage positive chondrocyte-like cells. Altogether these results indicate that SHP-2 is a cartilage tumor suppressor during aging.