WorldWideScience

Sample records for pteronyssinus dermatophagoides farinae

  1. Effect of a commercial air ionizer on dust mites Dermatophagoides pteronyssinus and Dermatophagoides farinae (Acari:Pyroglyphidae) in the laboratory

    Institute of Scientific and Technical Information of China (English)

    Suhaili Zainal Abidin; Ho Tze Ming

    2012-01-01

    Objective:To investigate the short and long term efficacy of a commercial air ionizer in killing Dermatophagoides pteronyssinus (D. pteronyssinus) and Dermatophagoides farinae (D. farinae) mites. Methods: The effect of a commercial ionizer on D. pteronyssinus and D. farinae was evaluated in the laboratory, using a specially designed test. Mortality was assessed after 6, 16 and 24 hours for direct exposure and after 24, 36, 48, 60 and 72 hours for exposure in simulated mattress. New batches of mites were used for each exposure time. Results: LT50 for direct exposure of ionizer was 10 hours for D. pteronyssinus and 18 hours for D. farinae. The LT50 for exposure in simulated mattress was 132 hours or 5.5 days for D. pteronyssinus and 72 hours or 3 days for D. farinae. LT95 for direct exposure of ionizer was 36 hours for D. pteronyssinus and D. farinae. Meanwhile, the LT95 for exposure in simulated mattress was 956 hours or 39.8 days for D. pteronyssinus and 403 hours or 16.8 days for D. farinae. Conclusions:This study demonstrates the increasing mite mortalities with increasing exposure time of a commercial ionizer and suggests that negative ions produced by an ionizer kill dust mites and can be used to reduce natural mite populations on exposed surfaces such as floors, clothes, curtains, etc. However, there is reduced efficacy on mites inside stuffed materials as in mattresses and furniture.

  2. p-Anisaldehyde: acaricidal component of Pimpinella anisum seed oil against the house dust mites Dermatophagoides farinae and Dermatophagoides pteronyssinus.

    Science.gov (United States)

    Lee, Hoi-Seon

    2004-03-01

    The acaricidal activity of anise seed oil-derived p-anisaldehyde and commercially available components of anise seed oil was examined against Dermatophagoides farinae and D. pteronyssinus and compared with those of the synthetic acaricides, benzyl benzoate and N,N-diethyl- m-toluamide (DEET). On the basis of LD 50 values, the compound most toxic to D. farinae adults was p-anisaldehyde (1.11 microg/cm2) followed by benzyl benzoate (9.32 microg/cm2), DEET (36.84 microg/cm2), 3-carene (42.10 microg/cm2), and estragol (43.23 microg/cm2). Against D. pteronyssinus adults, p-anisaldehyde (0.98 microg/cm2) was much more effective than benzyl benzoate (6.54 microg/cm2), DEET (17.79 microg/cm2), 3-carene (39.84 microg/cm 2), and estragol (40.11 microg/cm2). p-Anisaldehyde was about 8.4 and 6.7 times more toxic than benzyl benzoate against D. farinae and D. pteronyssinus adults, respectively. The results suggested that p-anisaldehyde may be useful as a lead compound for the development of new agents for the selective control of house dust mites.

  3. Group 1 Allergen Genes in Two Species of House Dust Mites, Dermatophagoides farinae and D. pteronyssinus (Acari: Pyroglyphidae: Direct Sequencing, Characterization and Polymorphism.

    Directory of Open Access Journals (Sweden)

    Rubaba Hamid Shafique

    Full Text Available Group 1 allergens of Dermatophagoides farinae (Der f 1 and D. pteronyssinus (Der p 1 dominate overall allergic responses in house dust mite allergy patients. The need for accurate identification and characterization of representative variants of group 1 allergens in any given geographic locality has been emphasized for development of appropriate allergen extracts. Regional amino acid sequence polymorphism has been described but the extent of this polymorphism is not well understood. Such data are completely absent for the USA and many other countries. Most previous studies used cDNA libraries generated by reverse transcriptase (RT-PCR and/or primers amplifying shorter fragments of this gene. Using novel species-specific primers and direct PCR, we document group 1 allergen gene sequence polymorphism in populations of D. farinae and D. pteronyssinus from the USA and Pakistan. We report two novel introns (nt pos 87 and 291 in both species, and the absence of intron 3 in Der p 1. Thirteen silent and one novel non-synonymous mutation (Tryptophan W197 to Arginine R197 were detected in D. farinae. The potential medical significance of the latter mutation is discussed. Two haplotypes of the Der f 1 gene were identified, haplotype 1 (63% was more frequent than haplotype 2 (18%. Polymorphism in Der f 1 displayed geographical localization, since both haplotypes were present in mite populations from Pakistan whereas haplotype 1 was observed only in the USA. In Der p 1, a silent mutation at nt (aa position 1011(149 and four non-synonymous mutations at positions 589(50, 935(124, 971(136, 1268(215 were observed. These mutations were reported from many other geographic regions, suggesting that polymorphism in the Der p 1 gene is panmictic. The extent of polymorphism in both genes is substantially lower than that reported previously (0.10-0.16% vs 0.31-0.49%, indicating the need for careful evaluation of potential polymerase errors in studies utilizing RT-PCR.

  4. Impacto de la inmunoterapia subcutánea con Dermatophagoides farinae y Dermatophagoides pteronyssinus sobre la calidad de vida de pacientes con rinitis y asma alérgica

    Directory of Open Access Journals (Sweden)

    Juan José Yepes-Núñez

    2014-06-01

    Full Text Available Introducción. Como sucede en otras partes del mundo, la prevalencia de asma y rinitis alérgica en Colombia está en aumento. Se ha establecido que la inmunoterapia subcutánea con alérgenos es eficaz a largo plazo en pacientes con rinitis alérgica y asma sensibilizados a Dermophagoides. Objetivo. Proveer evidencia sobre los cambios relacionados con la calidad de vida inducidos por la inmunoterapia subcutánea en sujetos con alergia respiratoria. Materiales y métodos. Se seleccionaron 76 sujetos con diagnóstico de alergia respiratoria con sensibilización a Dermatophagoides farinae y Dermatophagoides pteronyssinus. Para la evaluación de la calidad de vida se emplearon los instrumentos Kidscreen-27 y SF-36 (Short form 36. Estos instrumentos se aplicaron en dos ocasiones: durante la primera visita, en la cual se iniciaba la inmunoterapia subcutánea, y un año después de haberse iniciado el tratamiento. Resultados. Al año de estar recibiendo la inmunoterapia, los 22 sujetos que completaron el estudio presentaron cambios positivos en términos de calidad de vida. En los niños, el principal cambio se presentó en el dominio del ‘entorno escolar’ mientras que en los adultos fue en el de la ‘función física’. Discusión. Se evaluaron por primera vez en Colombia los beneficios inducidos por la inmunoterapia subcutánea para ácaros de polvo en la calidad de vida de sujetos con rinitis alérgica y asma mediante los cuestionarios Kidscreen-27 y SF-36. Los resultados proveen evidencia de que la inmunoterapia subcutánea influye positivamente en la calidad de vida en sujetos con rinitis asmática y asma sensibilizados a los ácaros de polvo.

  5. Dermatophagoides farinae Allergens Diversity Identification by Proteomics*

    OpenAIRE

    2013-01-01

    The most important indoor allergens for humans are house dust mites (HDM). Fourteen Dermatophagoides farinae allergens (Der f 1–3, 6, 7, 10, 11, 13–18, and 22) are reported although more than 30 allergens have been estimated in D. farinae. Seventeen allergens belonging to 12 different groups were identified by a procedure of proteomics combined with two-dimensional immunoblotting from D. farina extracts. Their sequences were determined by Edman degradation, mass spectrometry analysis, and cDN...

  6. Acaricidal effects of herb essential oils against Dermatophagoides farinae and D. pteronyssinus (Acari: Pyroglyphidae) and qualitative analysis of a herb Mentha pulegium(pennyroyal).

    Science.gov (United States)

    Rim, In-Sook; Jee, Cha-Ho

    2006-06-01

    This experiment was undertaken to screen the acaricidal effects of herb essential oils (pennyroyal, ylang ylang, citronella, lemon grass, tea tree, and rosemary) at different doses (0.1, 0.05, 0.025, 0.0125, and 0.00625 microliter/cm(2)) and exposure times (5, 10, 20, 20, 30 and 60 min) on house dust mites Dermatophgoides farinae and D. pteronyssinus. The most effective acaricidal components of pennyroyal (Mentha pulegium) were analyzed using a gas chromatography-mass spectrometer (GC-MS). Of these essential oils, the most effective was pennyroyal, which is composed essentially of pulegone (> 99%), at a dose of 0.025 microliter/cm(2), which at an exposure time of 5 min killed more than 98% of house dust mites. In the pennyroyal fumigation test, the closed method was more effective than the open method and maximum acaricidal effect was 100% at 0.025 microliter/cm(2), 60 min. The results show that herb essential oils, in particular, pennyroyal was proved to have potent acaricidal activity.

  7. Dermatophagoides farinae allergens diversity identification by proteomics.

    Science.gov (United States)

    An, Su; Chen, Lingling; Long, Chengbo; Liu, Xiaoyu; Xu, Xuemei; Lu, Xingre; Rong, Mingqiang; Liu, Zhigang; Lai, Ren

    2013-07-01

    The most important indoor allergens for humans are house dust mites (HDM). Fourteen Dermatophagoides farinae allergens (Der f 1-3, 6, 7, 10, 11, 13-18, and 22) are reported although more than 30 allergens have been estimated in D. farinae. Seventeen allergens belonging to 12 different groups were identified by a procedure of proteomics combined with two-dimensional immunoblotting from D. farina extracts. Their sequences were determined by Edman degradation, mass spectrometry analysis, and cDNA cloning. Their allergenicities were assayed by enzyme-linked immunosorbent assay inhibition tests, immunoblots, basophil activation test, and skin prick tests. Eight of them are the first report as D. farinae allergens. The procedure of using a proteomic approach combined with a purely discovery approach using sera of patients with broad IgE reactivity profiles to mite allergens was an effective method to investigate a more complete repertoire of D. farinae allergens. The identification of eight new D. farinae allergens will be helpful for HDM allergy diagnosis and therapy, especially for patients without response for HDM major allergens. In addition, the current work significantly extendedthe repertoire of D. farinae allergens.

  8. Activity of allergenic proteins from Dermatophagoides pteronyssinus

    Energy Technology Data Exchange (ETDEWEB)

    Wahn, U.; Mueller-Krampe, B.; Lind, P.

    1985-01-01

    Two purified allergens from Dermatophagoides pteronyssinus, Dp 42 (identical to P1) and Dp X were studied for their ability to induce histamine release from washed leukocytes and to bind to IgE antibodies from the serum of 27 mite-sensitive children. Almost all patients were demonstrated to be sensitive to both proteins by both assays. Dp 42 was found to have the highest allergenic activity, releasing histamine from leukocytes at a median concentration 10 times lower than for Dp X. There was a positive correlation between basophil sensitivity to both proteins and allergen specific serum IgE concentrations.

  9. Allergenicity assay of allergen from Dermatophagoides farinae in transgenic tobacco

    Institute of Scientific and Technical Information of China (English)

    TANG Mingjuan; SHEN Ye; HU Yuanlei; CAO Lei; NI Ting; ZHANG Hongyu; LIN Zhongping

    2004-01-01

    Derf2 gene for one of mite allergens in Dermatophagoides farinae has been cloned and expressed under regulation of 35S promoter in transgenic tobacco. The transcriptional analysis showed that this mite complete gene structure in genomic sequence could be spliced at prediction site. Allergenicity assay with immunological sera indicated that the extracts from the transgenic tobacco gave obvious positive IgE binding reaction with specific serum pool. This work would be of potential use in allergenicity assessment of genetically modified food.

  10. Development of ELISA for diagnosis of allergy to Dermatophagoides farinae.

    Science.gov (United States)

    Ho, T M; Radha, K; Shahnaz, M; Singaram, S P

    1993-09-01

    An indirect enzyme linked immunosorbent assay (ELISA) was developed for the diagnosis of allergy to a house dust mite, Dermatophagoides farinae. The efficacy of the ELISA was then evaluated against a prick test using a commercial allergen. Eighty five suspected allergic rhinitis patients from the Otorhinolaryngology Department, Kuala Lumpur General Hospital, were tested with the ELISA and prick test. Prick test and ELISA results were positive in 84.7% and 80.0% of the patients respectively. The ELISA was found to have 87.5% sensitivity, 61.5% specificity, 92.6% positive predictive value, 47.1% negative predictive value, 7.4% false positive and 52.9% false negative. There was total agreement between the prick test and ELISA for prick test grades of 3+ and 4+. It is concluded that the ELISA is a useful assay for detection of individuals who are highly sensitive to D. farinae.

  11. Pheromonal Communication in the European House Dust Mite, Dermatophagoides pteronyssinus

    Directory of Open Access Journals (Sweden)

    Johannes L.M. Steidle

    2014-08-01

    Full Text Available Despite the sanitary importance of the European house dust mite Dermatophagoides pteronyssinus (Trouessart, 1897, the pheromonal communication in this species has not been sufficiently studied. Headspace analysis using solid phase micro extraction (SPME revealed that nerol, neryl formate, pentadecane, (6Z,9Z-6,9-heptadecadiene, and (Z-8-heptadecene are released by both sexes whereas neryl propionate was released by males only. Tritonymphs did not produce any detectable volatiles. In olfactometer experiments, pentadecane and neryl propionate were attractive to both sexes as well as to tritonymphs. (Z-8-heptadecene was only attractive to male mites. Therefore it is discussed that pentadecane and neryl propionate are aggregation pheromones and (Z-8-heptadecene is a sexual pheromone of the European house dust mite D. pteronyssinus. To study the potential use of pheromones in dust mite control, long-range olfactometer experiments were conducted showing that mites can be attracted to neryl propionate over distances of at least 50 cm. This indicates that mite pheromones might be useable to monitor the presence or absence of mites in the context of control strategies.

  12. Comparison and Evaluation of Several Dermatophagoides Pteronyssinus Allergen Extracts for Skin Prick Test

    Institute of Scientific and Technical Information of China (English)

    Jin-lu Sun; Rui-qi Wang; Jia Yin; Liang-lu Wang; Hong-yu Zhang; Hong Li; Yu-xiang Zhi; Li-ping Wen; Kai Guan; Jian-qing Gu; Hai-juan He; Ying Zhao; Wei Zhang; Guo-qiang Sun

    2009-01-01

    To evaluate the significance of several Dermatophagoides pteronyssinus allergen extracts for skin prick test (SPT) in patients allergic to Dermatophagoides pteronyssinus.Methods Two hundred and nineteen patients enrolled in Peking Union Medical College Hospital underwent SPT and serum specific IgE assay to detect the Dermatophagoides pteronyssinus allergen. Three kinds of house dust mite allergen extracts were used for SPT, including the Dermatophagoides pteronyssinus extract prepared by our laboratory (group A), standardized Dermatophagoides pteronyssinus extract (group B), and mixed extracts of Dermatophagoides pteronyssinus and Dermatophagoidesfarinae (group C). Human serum spe-cific IgE result was regarded as the reference standard for diagnosis of Dermatophagoides pteronyssinus allergy.The receiver operating characteristic (ROC) curve was used to evaluate the diagnostic performance of SPT with the extracts of three groups.Results SPT results showed that the median wheal diameter of group A, group B, and group C was 0.43, 0.35, and 0.28 cm, respectively, with significant difference among three groups (P0.05). There was no local urticaria or systemic allergic reactions following the procedure of SPT. Local reaction was observed in 5 patients and delayed reaction was in 2 patients of group A. As for group B and C, local reaction occurred in 3 cases and delayed reaction in 2 cases in each group. The area un-der ROC curve of SPT with extract in group A, group B, and group C was 0.765, 0.801, and 0.782, respec-tively. Based on the detection results of serum specific IgE, the sensitivity of SPT in diagnosis of Dermato-phagoides pteronyssinus allergy with extract of group A, group B, and group C was 92.40/0, 87.00/0, and 81.5%,and the specificity was 60.6%, 73.2%, and 74.8%, respectively.

  13. Sensibilización a Dermatophagoides pteronyssinus, Dermatophagoides siboney y Blomia tropicalis en niños de tres consultorios Sensitization to Dermatophagoides pteronyssinus, Dermatophagoides siboney and Blomia tropicalis in children from three family physician’s offices

    Directory of Open Access Journals (Sweden)

    Raúl Lázaro Castro Almarales

    2005-08-01

    Full Text Available La morbilidad de las enfermedades alérgicas mediadas por el anticuerpo IgE, se ha incrementado en los últimos 40 años, y además, se han identificado a los ácaros domésticos como sus principales agentes causales. En Cuba, los ácaros más frecuentemente son: Dermatophagoides pteronyssinus (D. pteronyssinus, Dermatophagoides siboney (D. siboney y Blomia tropicalis (B. tropicalis. En este estudio investigamos si la sensibilización a estos ácaros constituye un problema de salud en nuestra población infantil, y su relación con la exposición al polvo doméstico. Se seleccionaron 103 niños alérgicos del pueblo de Guatao, que se clasificaron según su grado de exposición al polvo doméstico. A todos se les realizó la prueba por punción cutánea por duplicado en ambos antebrazos y se utilizaron los extractos alergénicos estandarizados VALERGEN-BT Ò (Blomia tropicalis, VALERGEN-DS Ò (Dermatophagoides siboney y VALERGEN-DP Ò (Dermatophagoides pteronyssinus, todos de producción nacional en el Centro Nacional de Biopreparados. La sensibilización fue mayor de forma significativa (pMorbidity of allergic diseases mediated by the IgE antibody has increased in the last 40 years and the house mites have been identified as their main causal agents. In Cuba, the most common mites are Dermatophagoides pteronyssinus (D. pteronyssinus, Dermatophagoides siboney (D. siboney and Blomia tropicalis (B. tropicalis. In this study, it was investigated if the sensitization to these mites is a health problem in our infantile population and its connection with the exposure to house dust. 103 allergic children from the Guatao town were selected. All of them underwent the skin puncture test in both forearms and the VALERGEN-BT Ò (Blomia tropicalis, VALERGEN-DS Ò (Dermatophagoides siboney and VALERGEN-DP Ò (Dermatophagoides pteronyssinus standardized allergenic extracts, which were produced in our country by the National Centre of Biopreparations, were used

  14. Der f 21, a novel allergen from dermatophagoides farina.

    Science.gov (United States)

    Wu, Yulan; Jiang, Congli; Li, Meng; Yu, Haiqiong; Xiao, Xiaojun; Fan, Xiaoqin; Lin, Jianli; Liu, Xiaoyu; Zhang, Min; Yang, Pingchang; Liu, Zhigang

    2016-01-01

    The Dermatophagoides farina (D. farina) allergens are an important factor contributing to allergic disease. To identify new allergens is important for diagnosis and treatment of allergic diseases. In this study, we sought to characterize the biological activity of Der f 21 of D. farina. The recombinant Der f 21 protein was characterized by western-blot, ELISA and Skin prick test using clinic patient's serum.An allergic asthma mouse model was established with the rDer f 21 as a specific antigen. The results showed that the sera from 28.9% in 38 dust mite allergic children displayed positive results in response to rDer f 21, and 42% in 98 dust mite allergic patients displayed positive response in skin prick test. In addition, Immune inhibition assays showed there was IgE cross-reactivity between rDer f 21 and rDer f 5. Moreover, an allergic asthma mouse model was established. Airway hyperresponsiveness, serum specific IgE, IgG1, eosinophil infiltration in the allergic mice, interleukin-4(IL-4) and interferon-γ (INF-γ) from spleen cells were markedly increased in the allergic mice. The results demonstrate that Der f 21 is a novel allergen.

  15. Comparison of Three Methods of Protein Extraction from Dermatophagoides Pteronyssinus for Two-dimensional Electrophoresis

    Institute of Scientific and Technical Information of China (English)

    Jin-lu Sun; Hong-yu Zhang; Zhi-yi Guo; Wan-tao Ying; Xiao-hong Qian; Jing-lan Wang

    2009-01-01

    Objective To explore an effective method of Dermatophagoides pteronyssinus protein extraction suitable for two-dimensional electrophoresis (2-DE) analysis. Methods The extracts of Dermatophagoides pteronyssinus were prepared with Coca's solution, lysis buffer of 2-DE, and Trizol reagent, respectively. Bicinchoninic acid (BCA) assay was used to determine the total protein concentration of the samples. The efficiency of different protein extraction methods were evaluated with 2-DE analysis. Results The concentrations of extracted protein by methods of Coca's solution, lysis buffer, and Trizol reagent were 0.63 g/L, 0.90 g/L, and 0.80 g/L, respectively. The 2-DE analysis results showed that some protein spots in low molecular weight (LMW) range could be detected with the Coca's solution method. With the lysis buffer of 2-DE method, more protein spots in LMW range could be detected, while the medium molecular weight (MMW) protein spots were absent. Several MMW protein spots (174-178 kD and 133 kD) and more LMW protein spots were detected with Trizol reagent method. Conclusions Among Coca's solution, lysis buffer of 2-DE, and Trizol reagent, the concentration of extracted protein of Dermatophagoides pteronyssinus by lysis buffer of 2-DE is the highest. However, most protein components of Dermatophagoides pteronyssinus purified mite bodies can be extracted by Trizol reagent, which may generally reflect the whole profile of Dermatophagoides pteronyssinus allergens.

  16. Gene silencing by RNA interference in the house dust mite, Dermatophagoides pteronyssinus.

    Science.gov (United States)

    Marr, Edward J; Sargison, Neil D; Nisbet, Alasdair J; Burgess, Stewart T G

    2015-12-01

    This is the first report of gene silencing by RNA interference (RNAi) in the European house dust mite, Dermatophagoides pteronyssinus, Trouessart, 1897. Using a non-invasive immersion method first developed for the honey bee mite, Varroa destructor, a significant reduction in the expression of D. pteronyssinus glutathione-S-transferase mu-class 1 enzyme (DpGST-mu1) was achieved following overnight immersion in double stranded RNA encoding DpGST-mu1. Although no detrimental phenotypic changes were observed following silencing, this technique can now be used to address fundamental physiological questions and assess the potential therapeutic benefit in silencing D. pteronyssinus target genes in selected domestic situations of high human-mite interface.

  17. Reduction of the IgE-binding ability and maintenance of immunogenicity of gamma-irradiated Dermatophagoides farinae

    Science.gov (United States)

    Lee, Ju-Woon; Seo, Ji-Hyun; Kim, Jae-Hun; Lee, Soo-Young; Park, Joong-Won; Byun, Myung-Woo

    2007-11-01

    House dust mites, Dermatophagoides farinae (DF) and Dermatophagoides pteronyssinus, are major allergens in the most common indoor allergen and are important risk factor for asthma. The modified antigen has been studied to treat allergic disorder. This study was carried out to measure possibility of modified allergen using gamma irradiation to treat allergy such as asthma. DF solutions (2 mg/ml) as target allergen were irradiated with Co-60 at 50 and 100 kGy. Conformational alternation of irradiated DF was observed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Levels of anti-irradiated DF mouse IgGs (sub-isotypes) against intact DF were measured similar to that of anti-intact DF IgGs. The binding abilities of house dust mite-allergic patients' IgE were reduced depending on radiation dose, and irradiation could inhibit the binding ability of patients' IgE more than 40%. This study has shown that the binding ability of IgE was reduced by conformational alteration by irradiation and the irradiated DF had epitopes capable to induce immunogeniciy.

  18. Reduction of the IgE-binding ability and maintenance of immunogenicity of gamma-irradiated Dermatophagoides farinae

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Ju-Woon; Seo, Ji-Hyun; Kim, Jae-Hun [Radiation Application Research Division, Advanced Radiation Technology Institute, Jeongeup 580 185 (Korea, Republic of); Lee, Soo-Young [Department of Pediatrics, Ajou University School of medicine, Suwon 442 749 (Korea, Republic of); Park, Joong-Won [Department of Allergy and Immunology, Severance Hospital, Yonsei University, Seoul 120 752 (Korea, Republic of); Byun, Myung-Woo [Radiation Application Research Division, Advanced Radiation Technology Institute, Jeongeup 580 185 (Korea, Republic of)], E-mail: mwbyun@kaeri.re.kr

    2007-11-15

    House dust mites, Dermatophagoides farinae (DF) and Dermatophagoides pteronyssinus, are major allergens in the most common indoor allergen and are important risk factor for asthma. The modified antigen has been studied to treat allergic disorder. This study was carried out to measure possibility of modified allergen using gamma irradiation to treat allergy such as asthma. DF solutions (2 mg/ml) as target allergen were irradiated with Co-60 at 50 and 100 kGy. Conformational alternation of irradiated DF was observed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Levels of anti-irradiated DF mouse IgGs (sub-isotypes) against intact DF were measured similar to that of anti-intact DF IgGs. The binding abilities of house dust mite-allergic patients' IgE were reduced depending on radiation dose, and irradiation could inhibit the binding ability of patients' IgE more than 40%. This study has shown that the binding ability of IgE was reduced by conformational alteration by irradiation and the irradiated DF had epitopes capable to induce immunogeniciy.

  19. Activated charcoal suppresses breeding of the house dust mite, Dermatophagoides pteronyssinus, in culture.

    Science.gov (United States)

    Nam, Hae-Seon; Siebers, Robert; Lee, Sun-Hwa; Kim, Sung-Ho; Lee, Sang-Han; Crane, Julian

    2007-04-01

    House dust mite sensitized asthmatics are advised to practice allergen avoidance. Charcoal pillows are used in Korea with unsubstantiated claims regarding their efficacy in alleviating asthma symptoms. We tested the effects of activated charcoal on breeding of house dust mites in culture. Twenty live adult house dust mites (Dermatophagoides pteronyssinus) were inoculated, 10 replicates, on culture media containing 0%, 1%, 3%, 5%, 10%, and 20% activated charcoal and incubated at 25 degrees C and a relative humidity of 75%. After four weeks, the mean numbers of live house dust mites were 286, 176, 46, 16, 7, and 0 for the 0%, 1%, 3%, 5%, 10%, and 20% charcoal-containing culture media, respectively. Thus, activated charcoal suppresses breeding of house dust mites and offers a new promising method for house dust mite control.

  20. [Histamine liberation and specific IgE against Dermatophagoides pteronyssinus in parasitized patients].

    Science.gov (United States)

    Moneo, I; Puente, S; Subirats, M; Ruiz, A; Lozano, M; González-Muñoz, M

    1994-01-01

    We studied 98 patients with different parasitosis, without clinical symptoms of mite sensitization, most of them coming from Guinea. Histamine release to Dermatophagoides pteronyssinus was performed using whole blood. Specific IgE to the same antigen was measured by EAST, as well as by an immunodot with the same antigen extract employed for the histamine release test. Finally, the EAST positive sera were studied by immunoblotting. The presence of specific IgE by EAST could be proved in 31 patients, but these antibodies were nor detected by dot, blot and histamine release. On the other hand, only two patients showed a positive histamine release test to D. pteronyssinus and in these two cases the EAST to mites was negative. There was no relation between total IgE levels and specific IgE to mites. The presence of mite-specific IgE showed a significative association to the parasite Trichuris trichiura (odds ratio 3.09). This fact suggest that the specific IgE values found in this population can reflect some cross-reaction between parasites and allergens. It is the author's opinion that the same study should be performed in european patients in order to test the relationship between mite-specific antibodies and the presence of parasites, specially of Trichuris trichiura.

  1. Proteome, Allergenome, and Novel Allergens of House Dust Mite, Dermatophagoides farinae.

    Science.gov (United States)

    Choopong, Jintarat; Reamtong, Onrapak; Sookrung, Nitat; Seesuay, Watee; Indrawattana, Nitaya; Sakolvaree, Yuwaporn; Chaicumpa, Wanpen; Tungtrongchitr, Anchalee

    2016-02-05

    Dermatophagoides farinae mite is a predominant source of indoor allergens causing high incidence of allergy worldwide. People with different genetic background respond differently to the mite components, and thus the component-resolved diagnosis (CRD) is preferred to the conventional allergy test based on crude mite extract. In this study, proteome and culprit components in the D. farinae whole body extract that sensitized the allergic patients were studied by using SDS-PAGE (1DE) and 2DE-IgE immunoblotting followed by LC-MS/MS and database search for protein identification. From the 1DE, the mite extract revealed 105 proteins that could be classified into seven functionally different groups: allergens, structural components, enzymes, enzyme inhibitor, receptor proteins, transporters, and binding/regulatory/cell signaling proteins. From the 2DE, the mite extract produced 94 spots; 63 were bound by IgE in sera of 20 D. farinae allergic patients. One more protein that was not revealed by the 2DE and protein staining reacted with IgE in 2 allergic patients. Proteins in 40 spots could be identified as 35 different types. Three of them reacted to IgE of >50% of the allergic patients, and hence they are major allergens: tropomyosin or Der f 10 (75%), aconitate hydratase (70%), and one uncharacterized protein (55%). Aconitate hydratase is a novel D. farinae major allergen unraveled in this study. Several mite minor allergens that have never been previously reported are also identified. The data have clinical applications in the component-resolved diagnosis for tailor-designed allergen-specific immunotherapy.

  2. Effects of the fungus Aspergillus penicillioides on the house dust mite Dermatophagoides pteronyssinus: an experimental re-evaluation.

    Science.gov (United States)

    Hay, D B; Hart, B J; Douglas, A E

    1993-07-01

    In this report the widely-held view that house dust mites benefit from fungal contamination of the dietary substratum is re-examined. The performance of Dermatophagoides pteronyssinus (Acari: Pyroglyphidae) is documented over two successive generations in the presence or absence of the xerophilic fungus Aspergillus penicillioides (Hyphomycetales: Moniliaceae). This fungus reduced survival, development rate, adult length and fecundity of D. pteronyssinus. Detrimental effects of A. penicillioides were proportional to the fungal density. Despite the antagonistic effects of A. penicillioides, a requirement for the fungus was indicated by the poor performance of fungus-free mites in the second generation; sustained culture of D. pteronyssinus in the absence of fungi is probably not possible. It is suggested that fungi may alter the particulate nature of the substratum to the detriment of house dust mites, but also provide micronutrients deficient in the diet.

  3. Sensitization to Dermatophagoides pteronyssinus and Blomia tropicalis extracts and recombinant mite allergens in atopic Thai patients.

    Science.gov (United States)

    Trakultivakorn, Muthita; Nuglor, Tipaporn

    2002-12-01

    Mite surveys in Thailand indicated that Dermatophagoides pteronyssinus (Dp) is predominant, but so far there were no data available on Blomia tropicalis (Bt), which is prevalent in the Asia Pacific region. Skin prick testing (SPT) was performed in 40 atopic children, 45 atopic adults and 17 non-atopic volunteers. Skin reactions to Dp were found in 25/40 (62.5%) and 23/45 (51.1%); skin reactions to Bt were found in 15/40 (37.5%) and 18/45 (40%) in atopic children and adults, respectively. SPT to the major sensitizing allergens Der p 1, Der p 2, Der p 5, and Blo t 5 showed positive results in 14/40 (35%), 12/40 (30%), 1/40 (2.5%) and 4/40 (10%) of atopic children, and in 12/45 (26.7%), 13/45 (28.9%), 5/45 (11.1%), 6/45 (13.3%) of atopic adults, respectively. The results indicate that Dp is one of the major sources of allergy, while Bt is a minor one and that Der p 1 and Der p 2 are important mite allergens in Chiang Mai, Thailand.

  4. Reduction of Airway Hyperresponsiveness by KWLL in Dermatophagoides-pteronyssinus-Challenged Mice

    Directory of Open Access Journals (Sweden)

    Chih-Che Lin

    2013-01-01

    Full Text Available Urine therapy has been commonly practiced in ancient civilizations including those of India, China, and Greece. The traditional Chinese medicine KWLL, the precipitation of human urine, has been used in China to alleviate the symptoms of asthma for thousands of years. However, the mechanism of action by which KWLL exerts its immunotherapy is unclear. This study attempted to elucidate the pharmacology of KWLL in mice that had been challenged recurrently by Dermatophagoides pteronyssinus (Der p. BALB/c mice were orally administered KWLL (1 g/kg before an intratracheal (i.t. challenge of Der p. Allergic airway inflammation and remodeling were provoked by repetitive Der p (50 μg/mice challenges six times at 1 wk intervals. Airway hypersensitivity, histological lung characteristics, and the expression profiles of cytokines and various genes were assessed. KWLL reduced Der p-induced airway hyperresponsiveness and inhibited eosinophil infiltration by downregulating the protein expression of IL-5 in bronchoalveolar lavage fluid (BALF. It also inhibited neutrophil recruitment by downregulating IL-17A in BALF. KWLL effectively diminished inflammatory cells, goblet cell hyperplasia, and mRNA expression of IL-6 and IL-17A in the lung. The reduction by KWLL of airway inflammatory and hyperresponsiveness in allergic asthmatic mice was mediated via immunomodulation of IL-5, IL-6, and IL-17A.

  5. Cloning, bioinformatics analysis, and expression of the dust mite allergen Der f 5 of Dermatophagoides farinae

    Directory of Open Access Journals (Sweden)

    Yubao Cui

    2012-08-01

    Full Text Available Crude extracts of house dust mites are used clinically for diagnosis and immunotherapy of allergic diseases, including bronchial asthma, perennial rhinitis, and atopic dermatitis. However, crude extracts are complexes with non-allergenic antigens and lack effective concentrations of important allergens, resulting in several side effects. Dermatophagoides farinae (Hughes; Acari: Pyroglyphidae is one of the predominant sources of dust mite allergens, which has more than 30 groups of allergen. The cDNA coding for the group 5 allergen of D. farinae from China was cloned, sequenced and expressed. According to alignment using the VECTOR NTI 9.0 software, there were eight mismatched nucleotides in five cDNA clones resulting in seven incompatible amino acid residues, suggesting that the Der f 5 allergen might have sequence polymorphism. Bioinformatics analysis revealed that the matured Der f 5 allergen has a molecular mass of 13604.03 Da, a theoretical pI of 5.43 and is probably hydrophobic and cytoplasmic. Similarities in amino acid sequences between Der f 5 and allergens of other domestic mite species, viz. Der p 5, Blo t 5, Sui m 5, and Lep d 5, were 79, 48, 53, and 37%, respectively. Phylogenetic analysis indicated that Der f 5 and Der p 5 clustered together. Blo t 5 and Ale o 5 also clustered together, although Blomia tropicalis and Aleuroglyphus ovatus belong to different mite families, viz. Echimyopodidae and Acaridae, respectively.

  6. [Cloning, expression and identification of Der f7 gene from Dermatophagoides farinae and its immunological characteristics].

    Science.gov (United States)

    Zheng, Man-Yin; Wu, Yu-Lan; Yan, Hao; Ji, Kun-Mei; Liu, Zhi-Gang

    2013-10-01

    To clone and express Der f7 gene of Dermatophagoides farinae, and identify its immunogenicity. Total RNA was extracted from D. farinae mites. A reference sequence (Accession No. AY283292) was used to design specific primers. The Der f7 gene fragment was amplified by RT-PCR, and cloned into pET-32a vector. The recombinant plasmid was transformed into E. coli BL21 (DE3) and induced with IPTG for protein expression. The recombinant protein was purified by Ni2+ chelating affinity chromatography and analyzed by SDS-PAGE and Western blotting. The Der f7 gene fragment was about 650 bp, and shared 99% homology with the published one (Accession No. FJ436108). SDS-PAGE result showed its relative molecular weight (M(r)) of 23 000. The recombinant protein showed appropriate combination ability with IgE in sera of mite allergic patients. Der f 7 gene has been expressed in prokaryotic expression system and shows allergenicity.

  7. Acaricidal activities of some essential oils and their monoterpenoidal constituents against house dust mite, Dermatophagoides pteronyssinus (Acari: Pyroglyphidae)

    Institute of Scientific and Technical Information of China (English)

    SAAD El-Zemity; HUSSIEN Rezk; SAHER Farok; AHMED Zaitoon

    2006-01-01

    The acaricidal activities of fourteen essential oils and fourteen of their major monoterpenoids were tested against house dust mites Dermatophagoides pteronyssinus. Five concentrations were used over two different time intervals 24 and 48 h under laboratory conditions. In general, it was noticed that the acaricidal effect based on LC50 of either essential oils or monoterpenoids against the mite was time dependant. The LC50 values were decreased by increasing of exposure time. Clove,matrecary, chenopodium, rosemary, eucalyptus and caraway oils were shown to have high activity. As for the monoterpenoids,cinnamaldehyde and chlorothymol were found to be the most effective followed by citronellol. This study suggests the use of the essential oils and their major constituents as ecofriendly biodegradable agents for the control of house dust mite, D. pteronyssinus.

  8. In Silico Prediction of T and B Cell Epitopes of Der f 25 in Dermatophagoides farinae

    Directory of Open Access Journals (Sweden)

    Xiaohong Li

    2014-01-01

    Full Text Available The house dust mites are major sources of indoor allergens for humans, which induce asthma, rhinitis, dermatitis, and other allergic diseases. Der f 25 is a triosephosphate isomerase, representing the major allergen identified in Dermatophagoides farinae. The objective of this study was to predict the B and T cell epitopes of Der f 25. In the present study, we analyzed the physiochemical properties, function motifs and domains, and structural-based detailed features of Der f 25 and predicted the B cell linear epitopes of Der f 25 by DNAStar protean system, BPAP, and BepiPred 1.0 server and the T cell epitopes by NetMHCIIpan-3.0 and NetMHCII-2.2. As a result, the sequence and structure analysis identified that Der f 25 belongs to the triosephosphate isomerase family and exhibited a triosephosphate isomerase pattern (PS001371. Eight B cell epitopes (11–18, 30–35, 71–77, 99–107, 132–138, 173–187, 193–197, and 211–224 and five T cell epitopes including 26–34, 38–54, 66–74, 142–151, and 239–247 were predicted in this study. These results can be used to benefit allergen immunotherapies and reduce the frequency of mite allergic reactions.

  9. Cloning, expression, and analysis of the group 2 allergen from Dermatophagoides farinae from China

    Directory of Open Access Journals (Sweden)

    Cui Yu-bao

    2010-12-01

    Full Text Available To obtain the recombinant group 2 allergen product of Dermatophagoides farinae (Der f 2, the Der f 2 gene was synthesized by RT-PCR. The full-length cDNA comprised 441 nucleotides and was 99.3% identical to the reference sequence (GenBank AB195580. The cDNA was bound to vector pET28a to construct plasmid pET28a(+-Der f 2, which was transformed into E. coli BL21 and induced by IPTG. SDS-PAGE showed a specific band of about 14kDa in the hole cell lysate. s estiated by chroatography, about 3.86 g of the recobinant product as obtained, which conjugated with serum IgE from asthmatic children. The protein had a signal peptide of 17 amino acids. Its secondary structure comprised an alpha helix (19.86%, an extended strand (30.82%, and a random coil (49.32%. The subcellular localization of this allergen was predicted to be at mitochondria. Furthermore, its function was shown to be associated with an MD-2-related lipid-recognition (ML domain. The results of this study provide a solid foundation for large-scale production of the allergen for clinical diagnosis and treatent of allergic disorders.Com a finalidade de obter o produto recombinante do alergeno grupo 2 do Dermatophagoides farinae (Der f2, o gene Der f2 foi sintetizado por RT-PCR. O cDNA continha 441 nucleotídeos e era idêntico em 99,3% à sequência de referência (GenBank AB195580. O cDNA foi ligado ao vetor pET28a para construir o plasmídeo pET28a(+-Der f2, o qual foi introduzido por transformação em E. coli BL21 e induzido por IPTG. Em SDS-PAGE foi vista mia banda específica de 14 kDa no lisado celular. Conforme estimado por cromatografia, cerca de 3,86 mg do produto recombinante foi obtido, que reagia com IgE sérica de crianças asmáticas. A proteína continha um peptídeo sinal de 17 amino ácidos. Sua estrutura secundária consistia de uma alfa hélice (19,86%, uma fita estendida (30,82%, e uma sequência randômica (49,32%. A localização subcelular desse alergeno foi predita

  10. Effects of dermatophagoides pteronyssinus allergen-specific immunotherapy on the serum interleukin-13 and pulmonary functions in asthmatic children

    Institute of Scientific and Technical Information of China (English)

    CHEN Zhuang-gui; LI Ming; CHEN Yan-feng; JI Jing-zhi; LI Ya-ting; CHEN Wei; CHEN Fen-hua; CHEN Hong

    2009-01-01

    Background Airway remodeling is the specific pathological characteristics of asthma, which is related to the clinical symptoms, pulmonary function, and airway hyperreactivity. This study aimed at exploring the effects of dermatophagoides pteronyssinus allergen-specific immunotherapy (SIT) on the serum interleukin (IL)-13 and pulmonary functions in asthmatic children.Methods Fifty-eight pediatric asthma patients allergic to dust mite participated in this study. Thirty-five children received SIT with a standardized dermatophagoides pteronyssinus extract for one year (SIT group), and the other 23 children treated with inhaled corticosteroids (ICS group) according to the Global Initiative for Asthma (GINA) for one year. Serum levels of IL-13, IL-4 and interferon (IFN)-y were examined and the pulmonary functions were checked before and after the treatment.Results After the treatment, the number of emergency visiting for asthma attack in SIT group was significantly less than that in ICS group. The serum levels of IL-4 and IL-13 were clearly reduced, IFN-γ and the ratio of IFN-γ/IL-4 were significantly increased, the pulmonary functions (forced vital capacity (FVC), forced expiratory volume in one second percentage (FEV1%) and peak expiratory flow percentage (PEF%) were significantly improved in the SIT group.Meanwhile, IFN-y and the ratio of IFN-γ/IL-4 were greatly increased, but serum levels of IL-4 and IL-13 had less changes,the pulmonary functions (FVC, FEV1% and PEF%) were poorly improved in ICS group. The basic pulmonary functions in both groups were at the same level, which had made more improvement in SIT group than in ICS group one year later.Conclusions One year of dermatophagoides pteronyssinus SIT can significantly reduce the frequencies of emergency visiting for asthma attack and improve the pulmonary functions of children with allergic asthma, and that is attributed to SIT, which can reduce the levels of IL-4 and IL-13 and regulate the imbalance of the

  11. Effect of Activated Charcoal Fibers on the Survival of the House Dust Mite, Dermatophagoides pteronyssinus: A Pilot Study.

    Science.gov (United States)

    Nam, Hae-Seon; Lee, Sun-Hwa; Choi, Young-Jin; Park, Joon-Soo; Cho, Moon-Kyun; Lee, Sang-Han; Crane, Julian; Siebers, Robert

    2012-01-01

    House dust mites produce potent allergens that exacerbate asthma in sensitized patients, whom are recommended to practice allergen avoidance within their home environment. We tested the effect of activated charcoal impregnated fibers on house dust mite survival. One hundred live adult house dust mites (Dermatophagoides pteronyssinus) were added to eight culture dishes preequilibrated at room temperature (n = 4) and 70% humidity (n = 4) containing house dust mite food and active charcoal fibers. At 10 minute intervals, live and dead house dust mites were counted. All house dust mites instantly attached to the activated charcoal fibers and started to shrink almost immediately. There were no live house dust mites present as early as 40 minutes in some dishes while after 190 minutes all house dust mites were dead. In conclusion, activated charcoal fibers, if incorporated into bedding items, have the potential to control house dust mites in the indoor environment.

  12. Quantitative PCR-based genome size estimation of the astigmatid mites Sarcoptes scabiei, Psoroptes ovis and Dermatophagoides pteronyssinus

    Directory of Open Access Journals (Sweden)

    Mounsey Kate E

    2012-01-01

    Full Text Available Abstract Background The lack of genomic data available for mites limits our understanding of their biology. Evolving high-throughput sequencing technologies promise to deliver rapid advances in this area, however, estimates of genome size are initially required to ensure sufficient coverage. Methods Quantitative real-time PCR was used to estimate the genome sizes of the burrowing ectoparasitic mite Sarcoptes scabiei, the non-burrowing ectoparasitic mite Psoroptes ovis, and the free-living house dust mite Dermatophagoides pteronyssinus. Additionally, the chromosome number of S. scabiei was determined by chromosomal spreads of embryonic cells derived from single eggs. Results S. scabiei cells were shown to contain 17 or 18 small (S. scabiei and P. ovis were 96 (± 7 Mb and 86 (± 2 Mb respectively, among the smallest arthropod genomes reported to date. The D. pteronyssinus genome was estimated to be larger than its parasitic counterparts, at 151 Mb in female mites and 218 Mb in male mites. Conclusions This data provides a starting point for understanding the genetic organisation and evolution of these astigmatid mites, informing future sequencing projects. A comparitive genomic approach including these three closely related mites is likely to reveal key insights on mite biology, parasitic adaptations and immune evasion.

  13. The complete mitochondrial genome of the house dust mite Dermatophagoides pteronyssinus (Trouessart: a novel gene arrangement among arthropods

    Directory of Open Access Journals (Sweden)

    Vanholme Bartel

    2009-03-01

    Full Text Available Abstract Background The apparent scarcity of available sequence data has greatly impeded evolutionary studies in Acari (mites and ticks. This subclass encompasses over 48,000 species and forms the largest group within the Arachnida. Although mitochondrial genomes are widely utilised for phylogenetic and population genetic studies, only 20 mitochondrial genomes of Acari have been determined, of which only one belongs to the diverse order of the Sarcoptiformes. In this study, we describe the mitochondrial genome of the European house dust mite Dermatophagoides pteronyssinus, the most important member of this largely neglected group. Results The mitochondrial genome of D. pteronyssinus is a circular DNA molecule of 14,203 bp. It contains the complete set of 37 genes (13 protein coding genes, 2 rRNA genes and 22 tRNA genes, usually present in metazoan mitochondrial genomes. The mitochondrial gene order differs considerably from that of other Acari mitochondrial genomes. Compared to the mitochondrial genome of Limulus polyphemus, considered as the ancestral arthropod pattern, only 11 of the 38 gene boundaries are conserved. The majority strand has a 72.6% AT-content but a GC-skew of 0.194. This skew is the reverse of that normally observed for typical animal mitochondrial genomes. A microsatellite was detected in a large non-coding region (286 bp, which probably functions as the control region. Almost all tRNA genes lack a T-arm, provoking the formation of canonical cloverleaf tRNA-structures, and both rRNA genes are considerably reduced in size. Finally, the genomic sequence was used to perform a phylogenetic study. Both maximum likelihood and Bayesian inference analysis clustered D. pteronyssinus with Steganacarus magnus, forming a sistergroup of the Trombidiformes. Conclusion Although the mitochondrial genome of D. pteronyssinus shares different features with previously characterised Acari mitochondrial genomes, it is unique in many ways. Gene

  14. Analysis of allergic components of Dermatophagoides farinae%粉尘螨变应原的分析

    Institute of Scientific and Technical Information of China (English)

    赵绮华; 李文; 陈蕴光; 周萍; 于陆; 王锡忠

    2008-01-01

    目的 对粉尘螨(Dermatophagoides farinae)变应原进行分离和鉴定其特异性变应原组分. 方法 采用ELISA检测粉尘螨变应原的生物活性.按常规方法制备粉尘螨浸出液,经SDS-PAGE分离,测定各组分的相对分子质量(M,);同时用对螨过敏的60例病人混合血清(建立血清库)作探针进行Westem blot,鉴定其特异性变应原组分. 结果 SDS-PAGE显示粉尘螨有15条蛋白带,Mr在14×103-109×103之间,其中主带有9条,M,分别为109×103、100×103、86×103、62×103、56×103、36×103、28 x103、19×103、14×103;Western blot结果表明,浸出液中共有5条致敏条带,其M,分别为109×103、100×103、36×103、28 x 103、14×103;经ELISA检测粉尘螨浸出液具有生物活性. 结论 粉尘螨的特异性变应原有5条,分别为109×103、100×103、36×103、28×103、14×103,其浸出液有稳定的生物活性.该研究为开发适合我国人群的粉尘螨标准化试剂提供了试验依据.%Objective To investigate the specific allergen components of Dermatophagoides fari-nae.Methods ELISA assay was used to detect tlle bioactivity of allergen.The crude extract of Dermatoph-agoidesfarinae Was prepared,and separated by SDS-PAGE to determine the relative molecular weight(M,)of every component.The allergic components were detected by Westem blot with sera obtained from 60 dust Ⅱlite allergie patients.Results There were 15 protein bands on the SDS-PACE,the M,Was from 14×10'to 109×103.and 9 master tapes,the M.Was 109×103,100×103,86×10',62×103,56 x103,36×103,28×103,19×103.14×103,respectively.Westem blot assay showed that there were 5 bands of special al-lergen with the Mr Was 109×lO3,100 x 103,36×103,28×103,14×103,respectively.The ELISA showed that the crude extract of Dermatophagoides farinae had bioactivity.Conclusion There are 5 special allergens in Dermatophagoides farinace with the Mr of 109×103,100×103,36×103,28×103,14×103,respectively.Its extract has steady

  15. Purification and characterization of M-177, a 177 kDa allergen, from the house dust mite Dermatophagoides farinae

    Directory of Open Access Journals (Sweden)

    Akihiro Fujikawa

    1999-01-01

    Full Text Available A high molecular weight allergen, M-177, was recently discovered in the house dust mite, Dermatophagoides farinae (D. farinae. The aims of this study were to develop a conventional purification procedure for M-177 and then to analyze some of the immunochemical properties of M-177. Mite extracts obtained from purified mite bodies were a suitable material for preparing M-177, because the purified mite extract contained large amounts of M-177. The purification of this allergen from the extract was achieved by ethanol fractionation, anion exchange chromatography, and gel filtration chromatography. The purified antigen was immunochemically equivalent to that of a preparation obtained by a previous affinity method using an anti-Mag 3-immobilized column. The yield of this purification procedure was 36.8% of the initial amount of M-177 in the extract, 40-fold greater than that of the previous immunoaffinity method. Our purification method was useful for preparing this allergen. The purified M-177 reacted in skin tests in 11 of 16 miteallergic patients, compared to 10 of 16 with Der f 2. The amount of M-177 in the purified mite extract determined by enzyme-linked immunosorbent assay inhibition was as much as 0.95% of the total protein, which was higher than the amounts of Der f 1 (0.52% and Der f 2 (0.32%. The potent allergenic activity and large amount of M-177 in the mites indicate that it is an important mite allergen.

  16. House-dust mite allergy: mapping of Dermatophagoides pteronyssinus allergens for dogs by two-dimensional immunoblotting

    Science.gov (United States)

    Marques, Andreia Grilo; Pereira, Luísa Maria Dotti Silva; Goicoa, Ana; Semião-Santos, Saul José; Bento, Ofélia Pereira

    2015-01-01

    Introduction Specific immunotherapy has shown to be very useful for allergy control in dogs, with a common success rate ranging from 65% to 70%. However, this efficacy could probably be improved and the identification of individual allergomes, with the choice of more adequate molecular allergen pools for specific immunotherapy, being the strategy. Aim To map Dermatophagoides pteronyssinus (Der p) allergens for mite-sensitized atopic dogs, for better understanding how individual allergograms may influence the response to house-dust mite immunotherapy. Material and methods To identify the Der p mite allergome for dogs, 20 individuals allergic to dust-mites and sensitized to Der p, were selected. The extract from Der p was submitted to isoelectric focusing (IEF), one-dimensional (1-D) and two-dimensional (2-D) sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Separated proteins were blotted onto polyvinylidene difluoride (PVDF) membranes and immunoblottings were performed with patient sera. Allergen-bound specific IgE was detected. Results Eleven allergens were identified from isoelectric focusing (IEF), as well as from 1-D SDS PAGE. From 2-D SDS-PAGE, 24 spots were identified. Conclusions Several similarities were found between dog and human allergograms and no absolute correlation between sensitization and allergy was observed either. As in humans, different individual allergograms do not seem to implicate different clinical patterns, but may influence the response to specific immunotherapy. The molecular epidemiology approach in veterinary allergy management, by the characterization of individual patients’ allergoms and by choosing the best molecular allergen pool for each patient could also improve the efficacy of allergy immunotherapy. PMID:26015775

  17. Cloning,sequence analysis and expression in E.coli of the group 3 allergen of Dermatophagoides farinae

    Institute of Scientific and Technical Information of China (English)

    CUI Yu-bao; CAI Hong-xing; LI Li; ZHOU Ying; GAO Cui-xiang; SHI Wei-hong; YU Ming

    2009-01-01

    Background The dust mites,which are mostly represented by Dermatophagoides spp.(Acari:Pyroglyphidae),are the major sources of indoor allergens.Identification and characterization of these mite allergen molecules are an important step in the development of new effective diagnostic procedures and possible therapeutic strategies for allergic disorders associated with dust mites.Methods Total RNA was extracted from Derrnatophagoides farinae.The gene coding for Der f 3 was amplified by RT-PCR with the primers designed based on previous sequence published in GenBank.The target gene was cloned intermediately into pMD19-T plasmid and finally into plasmid pET28a(+),expressed in E.coli BL21 at the aid of the inducer isopropyI-D-thiogalactopyranoside(IPTG).The physicochemical properties,spatial structure of the allergen were analyzed with bioinformatics software.Results The cDNA coding for group 3 allergen of Dermatophagoides farinae from China was cloned and expressed successfully.Sequencing analysis showed that there were nineteen mismatched nucleotides in five Der f 3 cDNA clones in comparison with the reference(GenBank Accession No.AY283291),which resulted in deduced amino acid sequence incompatibility in eleven residues.Bioinformatics analysis revealed that the Der f 3 pro-protein was an extracellular hydrophobic protein,consisting of 259 amino acids with a 16 amino acid signal peptide.The protein was deduced to have three chymotrypsin active sites(53-68 AA,108-122 AA and 205-217 hA),one N-glycosylation site,one cAMP-and cGMP-dependent protein kinase phosphorylation site,four protein kinase C phosphorylation sites,two casein kinase Ⅱ phosphorylation sites,and five N-myristoylation sites.Conclusions Der f 3 is an extracellular hydrophobic protein which possesses multiple activation and phosphorylation sites.Polymorphism may exist in the Der f3 gene but this needs to be further confirmed in the future.

  18. Chlorella vulgaris Attenuates Dermatophagoides Farinae-Induced Atopic Dermatitis-Like Symptoms in NC/Nga Mice

    Directory of Open Access Journals (Sweden)

    Heerim Kang

    2015-09-01

    Full Text Available Atopic dermatitis (AD is a chronic and inflammatory skin disease that can place a significant burden on quality of life for patients. AD most frequently appears under the age of six and although its prevalence is increasing worldwide, therapeutic treatment options are limited. Chlorella vulgaris (CV is a species of the freshwater green algae genus chlorella, and has been reported to modulate allergy-inducible factors when ingested. Here, we examined the effect of CV supplementation on AD-like symptoms in NC/Nga mice. CV was orally administrated for six weeks while AD-like symptoms were induced via topical application of Dermatophagoides farinae extract (DFE. CV treatment reduced dermatitis scores, epidermal thickness, and skin hydration. Histological analysis also revealed that CV treatment reduced DFE-induced eosinophil and mast cell infiltration into the skin, while analysis of serum chemokine levels indicated that CV treatment downregulated thymus- and activation-regulated chemokine (TARC and macrophage-derived chemokine (MDC levels. In addition, CV treatment downregulated mRNA expression levels of IL-4 and IFN-γ. Taken together, these results suggest that CV extract may have potential as a nutraceutical ingredient for the prevention of AD.

  19. The Drinking Effect of Hydrogen Water on Atopic Dermatitis Induced by Dermatophagoides farinae Allergen in NC/Nga Mice

    Directory of Open Access Journals (Sweden)

    Rosa Mistica C. Ignacio

    2013-01-01

    Full Text Available Hydrogen water (HW produced by electrolysis of water has characteristics of extremely low oxidation-reduction potential (ORP value and high dissolved hydrogen (DH. It has been proved to have various beneficial effects including antioxidant and anti-inflammatory effects; however, HW effect on atopic dermatitis (AD, an inflammatory skin disorder, is poorly documented. In the present study, we examined the immunological effect of drinking HW on Dermatophagoides farinae-induced AD-like skin in NC/Nga mice. Mice were administered with HW and purified water (PW for 25 days. We evaluated the serum concentration of pro-inflammatory (TNF-α, Th1 (IFN-γ, IL-2, and IL-12p70, Th2 (IL-4, IL-5, and IL-10, and cytokine expressed by both subsets (GM-CSF to assess their possible relationship to the severity of AD. The serum levels of cytokines such as IL-10, TNF-α, IL-12p70, and GM-CSF of mice administered with HW was significantly reduced as compared to PW group. The results suggest that HW affects allergic contact dermatitis through modulation of Th1 and Th2 responses in NC/Nga mice. This is the first note on the drinking effect of HW on AD, clinically implying a promising potential remedy for treatment of AD.

  20. Topical application of rapamycin ointment ameliorates Dermatophagoides farina body extract-induced atopic dermatitis in NC/Nga mice.

    Science.gov (United States)

    Yang, Fei; Tanaka, Mari; Wataya-Kaneda, Mari; Yang, Lingli; Nakamura, Ayumi; Matsumoto, Shoji; Attia, Mostafa; Murota, Hiroyuki; Katayama, Ichiro

    2014-08-01

    Atopic dermatitis (AD), a chronic inflammatory skin disease characterized by relapsing eczema and intense prurigo, requires effective and safe pharmacological therapy. Recently, rapamycin, an mTOR (mammalian target of rapamycin) inhibitor, has been reported to play a critical role in immune responses and has emerged as an effective immunosuppressive drug. In this study, we assessed whether inhibition of mTOR signalling could suppress dermatitis in mice. Rapamycin was topically applied to inflamed skin in a murine AD model that was developed by repeated topical application of Dermatophagoides farina body (Dfb) extract antigen twice weekly for 7 weeks in NC/Nga mice. The efficacy of topical rapamycin treatment was evaluated immunologically and serologically. Topical application of rapamycin reduced inflammatory cell infiltration in the dermis, alleviated the increase of serum IgE levels and resulted in a significant reduction in clinical skin condition score and marked improvement of histological findings. In addition, increased mTOR phosphorylation in the lesional skin was observed in our murine AD model. Topical application of rapamycin ointment inhibited Dfb antigen-induced dermatitis in NC/Nga mice, promising a new therapy for atopic dermatitis.

  1. Chlorella vulgaris Attenuates Dermatophagoides Farinae-Induced Atopic Dermatitis-Like Symptoms in NC/Nga Mice.

    Science.gov (United States)

    Kang, Heerim; Lee, Chang Hyung; Kim, Jong Rhan; Kwon, Jung Yeon; Seo, Sang Gwon; Han, Jae Gab; Kim, Byung Gon; Kim, Jong-Eun; Lee, Ki Won

    2015-09-02

    Atopic dermatitis (AD) is a chronic and inflammatory skin disease that can place a significant burden on quality of life for patients. AD most frequently appears under the age of six and although its prevalence is increasing worldwide, therapeutic treatment options are limited. Chlorella vulgaris (CV) is a species of the freshwater green algae genus chlorella, and has been reported to modulate allergy-inducible factors when ingested. Here, we examined the effect of CV supplementation on AD-like symptoms in NC/Nga mice. CV was orally administrated for six weeks while AD-like symptoms were induced via topical application of Dermatophagoides farinae extract (DFE). CV treatment reduced dermatitis scores, epidermal thickness, and skin hydration. Histological analysis also revealed that CV treatment reduced DFE-induced eosinophil and mast cell infiltration into the skin, while analysis of serum chemokine levels indicated that CV treatment downregulated thymus- and activation-regulated chemokine (TARC) and macrophage-derived chemokine (MDC) levels. In addition, CV treatment downregulated mRNA expression levels of IL-4 and IFN-γ. Taken together, these results suggest that CV extract may have potential as a nutraceutical ingredient for the prevention of AD.

  2. The Drinking Effect of Hydrogen Water on Atopic Dermatitis Induced by Dermatophagoides farinae Allergen in NC/Nga Mice

    Science.gov (United States)

    Ignacio, Rosa Mistica C.; Kwak, Hyun-Suk; Yun, Young-Uk; Sajo, Ma. Easter Joy V.; Yoon, Yang-Suk; Kim, Cheol-Su; Kim, Soo-Ki

    2013-01-01

    Hydrogen water (HW) produced by electrolysis of water has characteristics of extremely low oxidation-reduction potential (ORP) value and high dissolved hydrogen (DH). It has been proved to have various beneficial effects including antioxidant and anti-inflammatory effects; however, HW effect on atopic dermatitis (AD), an inflammatory skin disorder, is poorly documented. In the present study, we examined the immunological effect of drinking HW on Dermatophagoides farinae-induced AD-like skin in NC/Nga mice. Mice were administered with HW and purified water (PW) for 25 days. We evaluated the serum concentration of pro-inflammatory (TNF-α), Th1 (IFN-γ, IL-2, and IL-12p70), Th2 (IL-4, IL-5, and IL-10), and cytokine expressed by both subsets (GM-CSF) to assess their possible relationship to the severity of AD. The serum levels of cytokines such as IL-10, TNF-α, IL-12p70, and GM-CSF of mice administered with HW was significantly reduced as compared to PW group. The results suggest that HW affects allergic contact dermatitis through modulation of Th1 and Th2 responses in NC/Nga mice. This is the first note on the drinking effect of HW on AD, clinically implying a promising potential remedy for treatment of AD. PMID:24348704

  3. Immunization of rabbits with nematode Ascaris lumbricoides antigens induces antibodies cross-reactive to house dust mite Dermatophagoides farinae antigens.

    Science.gov (United States)

    Nakazawa, Takuya; Khan, Al Fazal; Yasueda, Hiroshi; Saito, Akemi; Fukutomi, Yuma; Takai, Toshiro; Zaman, Khalequz; Yunus, Md; Takeuchi, Haruko; Iwata, Tsutomu; Akiyama, Kazuo

    2013-01-01

    There are controversial reports on the relationship between helminthic infection and allergic diseases. Although IgE cross-reactivity between nematode Ascaris antigens and house dust-mite allergens in allergic patients have been reported, whether Ascaris or the mite is the primary sensitizer remains unknown. Here we found that immunization of naïve animals with Ascaris lumbricoides (Al) antigens induced production of antibodies cross-reactive to mite antigens from Dermatophagoides farinae (Df). Sera from Bangladeshi children showed IgE reactivity to Ascaris and mite extracts. IgG from rabbits immunized with Al extract exhibited reactivity to Df antigens. Treatment of the anti-Al antibody with Df antigen-coupled beads eliminated the reactivity to Df antigens. In immunoblot analysis, an approximately 100-kDa Df band was the most reactive to anti-Al IgG. The present study is the first step towards the establishment of animal models to study the relationship between Ascaris infection and mite-induced allergic diseases.

  4. Perilla leaf extract prevents atopic dermatitis induced by an extract of Dermatophagoides farinae in NC/Nga mice.

    Science.gov (United States)

    Komatsu, Ken-Ichi; Takanari, Jun; Maeda, Takahiro; Kitadate, Kentaro; Sato, Takashi; Mihara, Yoshihiro; Uehara, Kaori; Wakame, Koji

    2016-12-01

    Perilla (Perilla frutescens Britton) leaf comprises many types of active components, mainly flavonoids, and acts as an anti-inflammatory agent in in vitro and in vivo atopic dermatitis (AD) models. We investigated the effects of orally administered perilla leaf extract (PLE) on the symptoms of AD induced by Dermatophagoides farinae extract (DFE) in NC/Nga AD model mice. The mice were allowed free intake of 0.5% PLE. Skin lesions were assessed, and blood was sampled from the caudal vein on days 0, 7, 14, 21, and 31. On day 31, all mice were sacrificed to obtain blood, skin, spleen, and intestinal tissue samples. The assessment scores of the skin lesions and total serum IgE levels of PLE-treated mice (PLE group) were significantly lower than DFE-treated mice (DFE group) on days 7, 14, and 21. On day 31, the serum periostin and thymus and activation-regulated chemokine (TARC) levels in the PLE group were significantly lower than those in the DFE group. Histological analysis of the skin revealed that hyperplasia of the epidermal and dermal layers and infiltration of inflammatory cells (cell infiltration in corium tissues) were suppressed by PLE. Periostin deposition was observed in the skin tissue obtained from the DFE group. Moreover, the CD4+/CD8+ ratio of splenic T cells was suppressed in the PLE group but not in the DFE group.

  5. Dermatophagoides farinae house dust mite allergen challenges reduce stratum corneum ceramides in an experimental dog model of acute atopic dermatitis.

    Science.gov (United States)

    Stahl, Jessica; Paps, Judy; Bäumer, Wolfgang; Olivry, Thierry

    2012-12-01

    Ceramides are essential stratum corneum (SC) lipids and they play a pivotal role in maintaining effective cutaneous barrier function. The present study aimed at determining the effect of a Dermatophagoides farinae house dust mite (Df-HDM) allergen challenge on SC ceramides of atopic dogs experimentally sensitized to these allergens. Six Df-HDM-sensitized atopic Maltese-beagle dogs were used. Prechallenge SC was obtained by cyanoacrylate stripping. One week later, the dogs were challenged topically with Df-HDM allergens, which resulted in mild to moderate inflammation 24 h later. Two weeks after challenge, SC of lesional and nonlesional skin was obtained. Finally, SC was collected from challenge sites 2 months after lesion resolution. The different SC lipids were quantified blindly by thin-layer chromatography. Significantly lower amounts of ceramides [AH], [AP], [AS], [NP], [EOP], [NS] and [EOS] were observed in lesional SC compared with prechallenge samples, while no significant effect was found on the amount of other lipids, including cholesterol and free fatty acids. The ceramide profile of nonlesional skin generally showed the same postchallenge reduction pattern. Ceramide amounts returned to normal within 2 months after lesion remission. These findings suggest that the allergic reactions caused by Df-HDM allergens lead to a selective reduction of SC ceramides, not only at sites of inflammation but also at sites away from those of allergen application. There is normalization of ceramide amounts after inflammation subsides. These observations suggest that the deficiency of ceramides observed in canine atopic skin occurs, at least in part, secondary to inflammation. © 2012 The Authors. Veterinary Dermatology © 2012 ESVD and ACVD.

  6. Immunopotentiator from Pantoea agglomerans Prevents Atopic Dermatitis Induced by Dermatophagoides farinae Extract in NC/Nga Mouse.

    Science.gov (United States)

    Wakame, Koji; Komatsu, Ken-Ichi; Inagawa, Hiroyuki; Nishizawa, Takashi

    2015-08-01

    Pantoea agglomerans LPS (immunopotentiator from Pantoea agglomerans 1: IP-PA1) has been reported to have anti-inflammatory effects in in vitro and in vivo models. The aim of the present study was to investigate the effects of orally-administered IP-PA1 on atopic dermatitis (AD) symptoms induced by Dermatophagoides farinae body extract (DFE) in NC/Nga mice. Using the NC/Nga AD murine model, mice were orally administered 0.1% (High) or 0.01% (Low) water-containing IP-PA1. Skin lesion assessment and blood collection from the caudal vein was performed on days 0, 7, 21 and 31. On day 31, all mice were sacrificed and blood, skin, spleen, as well as intestine samples, were obtained. Assessment score of the skin lesion and serum immunoglobulin E (IgE) level of both IP-PA1 groups were significantly lower than that of the DFE group on days 14 and 21. The serum periostin and thymus and activation-regulated chemokine (TARC) level of IP-PA1-Low group was significantly lower than that of the DFE group on day 31. On histological examination of the skin, hyperplasia of epidermal and dermal layers and infiltration of inflammatory cells were suppressed by IP-PA1 administration. Deposition of periostin was observed in the DFE group skin tissue. Moreover, the CD4(+)/CD8(+) ratio of splenic T-cells increased by IP-PA1 administration. IP-PA1 administration may have an inhibitory effect on AD skin lesions. Copyright© 2015 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.

  7. Prevalence of sensitization to the storage mites Acarus siro, Tyrophagus putrescentiae, and Lepidoglyphus destructor in allergic patients with different degrees of sensitization to the house-dust mite Dermatophagoides pteronyssinus

    NARCIS (Netherlands)

    van der Heide, S; Niemeijer, NR; Hovenga, H; de Monchy, JGR; Dubois, AEJ; Kauffman, HF

    The prevalence of sensitization to the storage mites Acarus sire (AS), Tyrophagus putrescentiae (TP), and Lepidoglyphus destructor (LD) was studied in 250 sera of patients with different degrees of sensitization to the house-dust mite Dermatophagoides pteronyssinus (DP) by measuring IgE binding to

  8. Prevalence of sensitization to the storage mites Acarus siro, Tyrophagus putrescentiae, and Lepidoglyphus destructor in allergic patients with different degrees of sensitization to the house-dust mite Dermatophagoides pteronyssinus

    NARCIS (Netherlands)

    van der Heide, S; Niemeijer, NR; Hovenga, H; de Monchy, JGR; Dubois, AEJ; Kauffman, HF

    1998-01-01

    The prevalence of sensitization to the storage mites Acarus sire (AS), Tyrophagus putrescentiae (TP), and Lepidoglyphus destructor (LD) was studied in 250 sera of patients with different degrees of sensitization to the house-dust mite Dermatophagoides pteronyssinus (DP) by measuring IgE binding to e

  9. Dermatophagoides in childhood asthma. Allergy to dermatophagoides associates more severe childhood asthma with a potential role for acaricides.

    Science.gov (United States)

    Adham, Tamer M; Tawfik, Safwat A

    2012-03-01

    To evaluate hypersensitivity to Dermatophagoides pteronyssinus (D. pteronyssinus) and Dermatophagoides farinae (D. farinae) in pediatric patients with asthma, and the use of acaricides. This is a randomized controlled trial in 82 asthmatic children. They were recruited and evaluated for severity and chronicity according to the Global Initiative for Asthma Guidelines. The study was carried out in the Pediatric Allergy Clinic, Al Noor Hospital, Khalifa Branch, Abu Dhabi, United Arab Emirates between September 2008 and June 2010. Skin prick test (SPT) was performed including D. pteronyssinus and D. farinae, and for those who were sensitive to one, or the other. The therapeutic value of acaricides was evaluated by assessing the severity of asthma before and after their use. Approximately 81.7% of asthmatics were sensitive to house dust mites (HDM). Hypersensitivity was associated with the more severe and persistent asthma (p=0.029). The manifested severity of asthma was not HDM species-specific. There was a linear association between response of children to acaricides and increasing severity of asthma. Hypersensitivity to HDM is an important factor for persistent and severe forms of asthma. Acaricides can help control childhood asthma, and we recommend SPT as part of the routine work-up of patients with asthma to determine HDM sensitive patients that can benefit from acaricides use.

  10. Cross‑reactivity between group-5 and -21 mite allergens from Dermatophagoides farinae, Tyrophagus putrescentiae and Blomia tropicalis.

    Science.gov (United States)

    Kim, Chung-Ryul; Jeong, Kyoung Yong; Yi, Myung-Hee; Kim, Hyoung-Pyo; Shin, Ho-Joon; Yong, Tai-Soon

    2015-10-01

    Group-5 and group-21 allergens, produced by house dust mites and storage mites are 36.6-55.8% identical in their sequences and are recognized by at least 50% of immunoglobulin (Ig)E from the sera of individuals allergic to dust mites. In the present study, recombinant group-5 and ‑21 allergens from three mite species, Dermatophagoides farinae (rDer f 5 and 21), Tyrophagus putrescentiae (rTyr p 5 and 21), and Blomia tropicalis (rBlo t 5 and 21), were purified from Escherichia coli, and the IgE reactivities and cross‑reactivities of these allergen variants were assessed. The IgE binding frequencies of rDer f 5, rDer f 21, rTyr p 5, rTyr p 21, rBlo t and rBlo t 21 proteins were 64.95, 65.98, 30.41, 41.24, 30.93 and 21.65%, respectively. The IgE reactivity of rDer f 5 correlated highly with that of rDer f 21 (r=0.733). rTyr p 5 exhibited the highest level of correlation with rTyr p 21 (r=0.950), while the correlation of rBlo t 5 with rBlo t 21 was the lowest observed (r=0.104). The binding of IgE to rDer f 5 and rDer f 21 was not inhibited by any allergens but themselves. While rDer f 5 inhibited only 60.3% of IgE binding to rBlo t 5, rDer f 21 exhibited a high inhibitory effect against rTyr p 5 (93.01%), rTyr p 21 (92.12%), rBlo t 5 (86.97%) and rBlo t 21 (70.30%), implying cross‑reactivity among mite species. The results of the present study demonstrated that the majority of the IgE reactivity to group-5 and -21 storage mite allergens is due to cross‑reaction. It is therefore imperative to develop an accurate, component‑resolved diagnosis for dust mite allergies.

  11. Comparative analysis of biological activities of Der p I-derived peptides on Fc epsilon receptor-bearing cells from Dermatophagoides pteronyssinus-sensitive patients.

    Science.gov (United States)

    Jeannin, P; Pestel, J; Bossus, M; Lassalle, P; Tartar, A; Tonnel, A B

    1993-01-01

    The ability of four uncoupled synthetic peptides (p52-71, p117-133, p176-187, p188-199) derived from Der p I, a major allergen from the house dust mite Dermatophagoides pteronyssinus (Dpt) to stimulate Fc epsilon R+ cells from Dpt-sensitive patients was comparatively analysed. Each free peptide may specifically stimulate basophils (Fc epsilon RI+ cells) and platelets (Fc epsilon RII+ cells) from patients with significant levels of anti-Der p I IgE antibodies; p52-71 and p117-133 appear the best cell stimulation inducers. Both concentration-dependent biological activities of Der p I-peptide on Fc epsilon R+ cells are enhanced by coupling peptide to a carrier (as human serum albumin). Interestingly each Der p I-sensitive patient tested presents an individual pattern of response to peptide. Thus, from our results it appears that different Der p I sequences could be involved in the immune response to Der p I. PMID:7682161

  12. Cloning and characterization of the group 5 allergens from Dermatophagoides farinae%粉尘螨变应原第5组分基因克隆及分子特征分析

    Institute of Scientific and Technical Information of China (English)

    崔玉宝; 彭江龙; 周鹰; 王颖; 孙炜

    2009-01-01

    目的 获得粉尘螨变应原第5组分的编码基因(Der f5)并了解其分子特征.方法 用RNAiso试剂盒提取粉尘螨总RNA,根据GenBank已公布的Der f5核酸序列设计引物,用RT-PCR扩增获得其编码基因,插入pMD19-T Simple载体进行序列测定和分析.结果 获得的Der f5基因与参考序列(GenBank AY283283)同源性达97.8%,含1个完整的开放阅读框架(ORF),由132个氨基酸组成,信号肽位于1~19AA、跨膜区域位于1~19AA,为细胞外疏水性蛋白.二级结构由延伸主链(1.52%)、无规则卷曲(7.58%)和α螺旋(90.91%)组成.具有酪蛋白激酶Ⅱ磷酸化位点2个.其氨基酸序列与屋尘螨变应原第5组分相似率为78%.结论 成功克隆了 Der f5,并初步预测得其分子特征.%Objective To obtain the gene coding for the group 5 allergens from Dermatophagoides farinae ( Derf5 ) and predict its molecular characteristics. Methods The total RNA of D. farinae were extracted, and the gene Derf5 was amplified by RT-PCR with the primers designed according to previous sequence published in GenBank. The target gene was linked into pMD19-T Simple plasmid, sequenced and analyzed by bioinformatics software. Results The sequence homology reached to 97.8% between our sequenced result with one complete open reading fragment (ORF) and the reference. The gene encode an extracellular hydrophobic protein with 132 amino acid resides, one signal peptide from 1 to 19 position and one transmembrane domain from 1 to 19 position. The secondary structure was composed of extended strand (1. 52% ), random coil (7.58%) and alpha helix (90.91%). The encoded protein was deduced to have two Casein kinase Ⅱ phosphorylation sites. The similarity of the amino acid sequence of the group 5 allergens were 78% between D. farinae and D. pteronyssinus. Conclusion The gene Derf5 was cloned successfully, and its characteristics was primarily predicted.

  13. 7,3′,4′-Trihydroxyisoflavone Ameliorates the Development of Dermatophagoides farinae-Induced Atopic Dermatitis in NC/Nga Mice

    Directory of Open Access Journals (Sweden)

    Bo-Bae Kim

    2013-01-01

    Full Text Available Atopic dermatitis is an inflammatory and chronically relapsing skin disorder that commonly occurs in children; the number of atopic dermatitis patients is increasing. The cause and mechanism of atopic dermatitis have not been defined clearly, although many studies are ongoing. Epidemiological studies suggest that soybean and its isoflavones have immunoregulatory activities. Here, we report that 7,3′,4′-trihydroxyisoflavone (7,3′,4′-THIF, a major metabolite of daidzin, effectively inhibited lipopolysaccharide (LPS-induced nitric oxide (NO, tumor necrosis factor (TNF-α, and interleukin (IL-6 production in RAW 264.7 cells, and also reduced β-hexosaminidase secretion in RBL-2H3 cells. Moreover, 7,3′,4′-THIF significantly reduced scratching time, transepidermal water loss, and mast cell infiltration. It also decreased protease-activated receptor (PAR-2 and IL-4 expression and increased filaggrin expression in skin lesions of NC/Nga mice. These results suggest that 7,3′,4′-THIF improves Dermatophagoides farina body extract-induced atopic dermatitis in NC/Nga mice.

  14. Theobroma cacao extract attenuates the development of Dermatophagoides farinae-induced atopic dermatitis-like symptoms in NC/Nga mice.

    Science.gov (United States)

    Kang, Heerim; Lee, Chang Hyung; Kim, Jong Rhan; Kwon, Jung Yeon; Son, Myoung-Jin; Kim, Jong-Eun; Lee, Ki Won

    2017-02-01

    Cacao beans from Theobroma cacao are an abundant source of polyphenols, particularly flavonoids. Previous studies demonstrated that cacao flavanols decrease pro-inflammatory cytokines resulting in the alleviation of allergic symptoms. We sought to investigate the effects of cacao extract (CE) on Dermatophagoides farinae extract (DFE)-induced atopic dermatitis (AD)-like symptoms. CE attenuated DFE-induced AD-like symptoms as assessed by skin lesion analyses, dermatitis score, and skin thickness. Histopathological analysis revealed that CE suppressed DFE-induced immune cell infiltration into the skin. These observations occurred concomitantly with the downregulation of inflammatory markers including serum immunoglobulin (Ig) E, chemokine; thymus and activation-regulated chemokine and macrophage-derived chemokine as well as the skin-derived cytokines interleukin (IL)-4, IL-5, and interferon-γ. CE also significantly alleviated transepidermal water loss and increased skin hydration. These results suggest that CE, a natural phytochemical-rich food, has potential therapeutic efficacy for the treatment of AD. Copyright © 2016. Published by Elsevier Ltd.

  15. CONSTRUCTION AND EXPRESSION OF DERMATOPHAGOIDES PTERONYSSINUS GROUP 1 MAJOR ALLERGEN T CELL FUSION EPITOPE PEPTIDE VACCINE VECTOR BASED ON THE MHC II PATHWAY.

    Science.gov (United States)

    Li, Chaopin; Zhao, Beibei; Jiang, Yuxin; Diao, Jidong; Li, Na; Lu, Wei

    2015-11-01

    Antecedentes y objetivo: el Dermatophagoides peteronyssinus es uno de los principales ácaros del polvo doméstico responsables del asma alérgica que se pueden administrar provisionalmente para una inmunoterapia específica. El presente estudio busca construir un vector que codifique epítopos de células T del grupo de alérgenos principal, el Grupo 1 de Dermatophagoides pteronyssinus como una vacuna suministrada mediante la vía MHC de clase II. Métodos: se sintetizaron las secuencias de nucleótidos de los 3 genes objetivo, incluyendo TAT, IhC y el fragmento recombinante de Der p 1 encargado de codificar 3 epítopos de célula T. Después de la amplificación de los 3 fragmentos objetivo por PCR y digestión con endonucleasas de restricción correspondientes, el gen recombinante TAT-IhC-Der p 1-3T se ligó usando T4 DNA ligasa y se insertó en el vector de expresión procariota pET28a (+) para construir el plásmido recombinante pET 28a (+)-TAT-IHC-Der p 1-3T, que se confirmó por digestión con endonucleasas de restricción y secuenciación. El vector recombinante se transformó en E. coli cepa BL21 (DE3) y se indujo con IPTG, y la proteína inducida TATIHC- Der p1-3T se detectó mediante SDS-PAGE. Después de la purificación, la proteina recombinante se confirmó por análisis de inmunotransferencia (Western blot) y se probó su alergenicidad usando el ensayo de unión a IgE. Resultados: el plásmido recombinante pET-28a-TATIHCDer p1-3T se construyó con éxito, se confirmó por digestión con endonucleasas de restricción y la secuenciación y la expresión de la proteína recombinante TAT-IHCDer p1-3T fue inducida en E. coli. Purificación con éxito verificada mediante Western blot de la proteína objetivo, que mostró una capacidad de unión a IgE más fuerte que Der p1. Conclusión: hemos construido con éxito el vector de expresión recombinante pET-28a-TAT-IHC-Der p1-3T que expresa una vacuna de epítopo de células T administrada por vía MHC II con

  16. Sensibilidad y especificidad de la prueba cutánea por punción con extractos alergénicos estandarizados de Dermatophagoides pteronyssinus en adultos

    Directory of Open Access Journals (Sweden)

    Raúl Lázaro Castro

    2013-08-01

    Full Text Available La prueba cutánea por punción (PCP, que utiliza extractos alergénicos estandarizados de ácaros, es una herramienta diagnóstica sensible y confiable. La sensibilización IgE a los ácaros domésticos es una de las principales causas de enfermedades alérgicas respiratorias. En Cuba las especies de ácaros más relevantes son: Dermatophagoides pteronyssinus (Dp, D. siboney y Blomia tropicalis. El objetivo de este trabajo fue determinar la eficacia diagnóstica de la PCP, utilizando un extracto alergénico estandarizado de Dp de producción nacional (VALERGEN-DP, BIOCEN y otros dos estándares comerciales foráneos (A: ALK, Holanda y B: Stallergenes, Francia. Se realizó un estudio analítico, conducido en 50 pacientes con antecedentes de alergia al polvo doméstico y IgE sérica positiva al Dp y 50 con iguales antecedentes, pero con IgE sérica negativa. Se realizaron réplicas de las punturas en ambos brazos, con una concentración de 20 000 UB/mL. En esta prueba con VALERGEN-DP el promedio del habón fue de 43,5 mm2, un valor superior estadísticamente significativo con respecto el producto A (p=0,002. La coincidencia diagnóstica entre los tres productos fue de 98-99%. La PCP con VALERGEN-DP mostró un 98% de especificidad y 82% de sensibilidad. No fueron detectadas diferencias significativas (p>0,05 entre los productos para estos parámetros. La eficacia diagnóstica de la PCP con el VALERGEN-DP fue similar a la que se obtuvo utilizando dos extractos estándar, lo que justifica su utilización para el diagnóstico de la sensibilización a Dp en pacientes con síntomas de alergia frente al polvo doméstico.

  17. Distribution and abundance of house dust mites, Dermatophagoides spp., in different ecological localities in Esna City, Kena Governorate, Egypt.

    Science.gov (United States)

    Yassin, M K; Rifaat, M M

    1997-08-01

    The distribution and abundance of house dust mites, Dermatophagoides spp. were studied in July, September and November, 1995 in three different localities in Esna City, Kena Governorate, Upper Egypt. During these months, 15 houses were sampled in each locality. 87% of riverside houses were infested with mites where D. pteronyssinus dominated (80%) over D. farinae. Sixty percent of the valley houses sampled were infested, where D. farinae was dominant (66%). Densities of both Dermatophagoides spp., were considerably higher in riverside than in valley houses. Live mites were not found in the lightly infested houses sampled in the desert area (54% positive). Relative humidity, which varied in houses located in different climatic localities in Esna City, was noted to be the principal limiting factor influencing the distribution and abundance of both species. Temperature did not appear to be an important factor influencing the distribution and abundance.

  18. Acaricidal activity of constituents identified in Foeniculum vulgare fruit oil against Dermatophagoides spp. (Acari: Pyroglyphidae).

    Science.gov (United States)

    Lee, Hoi-Seon

    2004-05-19

    Acaricidal activities of components derived from Foeniculum vulgare fruit oil against Dermatophagoides farinae and Dermatophagoides pteronyssinus were examined using direct contact application and compared with that of the commercial repellent benzyl benzoate. The major biologically active constituent of Foeniculum fruit oil was characterized as (+)-fenchone by spectroscopic analyses. On the basis of LD(50) values, the compound most toxic to D. farinae was p-anisaldehyde (11.3 mg/m(2)) followed by (+)-fenchone (38.9 mg/m(2)), (-)-fenchone (41.8 mg/m(2)), benzyl benzoate (89.2 mg/m(2)), thymol (90.3 mg/m(2)), and estragol (413.3 mg/m(2)). Against D. pteronyssinus, p-anisaldehyde (10.1 mg/m(2)) was much more effective than benzyl benzoate (67.5 mg/m(2)), thymol (68.5 mg/m(2)), and estragol (389.9 mg/m(2)). These results indicate that the acaricidal activity of F. vulgare fruit oil likely results from (+)-fenchone and p-anisaldehyde. (+)-Fenchone was 20.3 times more abundant in the oil than p-anisaldehyde. (+)-Fenchone and p-anisaldehyde merit further study as potential house dust mite control agents or as lead compounds.

  19. 上海粉尘螨变应原及特异性免疫治疗%Dermatophagoides farinae Mite Allergen and Specific Immunotherapy in Shanghai

    Institute of Scientific and Technical Information of China (English)

    温廷桓

    2009-01-01

    Studies on mite allergy had been launched by the Shanghai First Medical College since 1970's in this country.The preparations of SMU-Df from the local specimens of Dermatophagoides farinae(Df)in Shanghai have been shown the highest allergenic potency in comparison with that of the foreign ones,including the Df preparations from USFDA,VUS and ALK.Similar patterns of the protein curves were yielded by gel filtration,indicating almost similar allergenicities with both Df pure mite body and its spent culture medium.Around 80% of the allergic cases were sensitive to mite allergen and can be diagnosed by skin prick test,nasal provocation test and serum IgE level assay.Seasonal classic immunotherapy for allergic patients by Injectio Dermatophagoidei farinae,the first commercial allergen licensed by the Chinese government,achieved significant effect in relieving symptoms of allergic disorders in majority of cases,and long lasting effect of mite specific immunotherapy was also documented with minimal adverse reactions.Modifications of the Df crude extract and various modes of treatment have been studied.Studies on sublingual mite vaccine for mite allergic disorders developed synchronously with foreign trend since 1992.Sublingual drops were well acceptable by child cases almost without age restriction with higher efficacy.Rush schedule of mite immunotherapy led to a quick relief of allergic symptoms and long lasting curative effects.The Df allergen induced immunological regulation of human beings was established not only among the atopic patients,but also in healthy persons.%我国螨性变态反应的研究开始于1970年代上海第一医学院(现为复旦大学上海医学院).上海当地的粉尘螨螨种制备的变应原SMU-Df,其过敏活性与国外同种比较,超过许多倍属于最高的,包括美国食品药品管理局参考品、弗吉尼亚大学标准品、丹麦哥本哈根变态反应研究所标准质量品等.SMU-Df经凝胶层析所显示的蛋白

  20. 过敏性哮喘患儿含服粉尘螨滴剂临床分析%Clinical analysis in children with allergic asthma of dermatophagoides farinae drops containing clothing

    Institute of Scientific and Technical Information of China (English)

    张春林

    2013-01-01

    目的 探讨特异性免疫治疗(SIT)药物粉尘螨滴剂治疗儿童过敏性支气管哮喘的疗效.方法 将162例儿童过敏性支气管哮喘患者随机分成两组,治疗组对84例含服脱敏药物治疗,对照组78例单纯吸入药物,评估两组疗效及其对肺功能的影响.结果 经36周后,治疗组肺功能及发作次数明显优于对照组.结论 舌下含服粉尘螨滴剂治疗儿童过敏性哮喘患者疗效显著.%Objective To investigate the curative effective of specific immunotherapy drugs dermatophagoides farinae drops on children with allergic asthma.Methods One hundred and sixty-two children with allergic asthma were divided into two groups randomly.The 84 patients in treatment group were treated with buccaled desensitizing drug.The 78 patients in control group were treated with inhalation drug simply.The curative effective and influence on lung function were evaluated.Results After 36 weeks of treatment,the treatment group was obviously better than the control group in lung function and attack frequency.Conclusions The curative effective of dermatophagoides farinae drops on children with allergic asthma is remarkable.

  1. Mise au point d'une forme stable de l'allergène Der p 3 de Dermatophagoides pteronyssinus pour le diagnostic rapide et le développement de nouvelles approches d'immunothérapie de l'allergie aux acariens

    OpenAIRE

    Bouaziz, Ahlem

    2014-01-01

    L’allergie aux acariens touche 20 à 30 % de la population occidentale. L’allergène Der p 3 de l’acarien Dermatophagoides pteronyssinus est une protéase à serine active appartenant à la famille de la trypsine. Son activité protéolytique pourrait être un facteur adjuvant impliqué dans le développement et la chronicité de l’allergie. A l’heure actuelle, l’implication de Der p 3 dans l’allergie est peu connue et son taux de fixation des IgE provenant de patients allergiques est controversé. Cepe...

  2. O repertório de alergénios do ácaro Dermatophagoides pteronyssinus para o cão – Sensibilização a quê? Imunoterapia (específica) para o quê?

    OpenAIRE

    Martins, Luís; Marques,Andreia; Pereira, Luísa; Semião-Santos, Saul; Malaman, Maria; Bento, Ofélia

    2013-01-01

    Objetivo: Identificar os repertórios alergénicos mais relevantes para o ácaro Dermatophagoides pteronyssinus (Der p) em cão, de forma a permitir importantes inovações em termos de opções imunoterápicas dirigidas, com ganho de eficácia. Métodos: Da consulta externa de dermatologia e alergia dos Hospitais Veterinários da Universidade de Évora e da Fundação Rof Codina (Lugo, Espanha) selecionaram-se 20 cães, maioritariamente indoor e com sintomatologia perenal, alérgicos aos ácaros e sensibil...

  3. Acaricidal activity of active constituent isolated in Chamaecyparis obtusa leaves against Dermatophagoides spp.

    Science.gov (United States)

    Jang, Young-Su; Lee, Chi-Hoon; Kim, Moo-Key; Kim, Jeong-Hak; Lee, Sang-Hyun; Lee, Hoi-Seon

    2005-03-23

    Acaricidal activities of materials derived from Chamaecyparis obtusa leaves against Dermatophagoides farinae and Dermatophagoides pteronyssinus were examined using the dry film method and compared with that of commercial benzyl benzoate and N,N-diethyl-m-toluamide (DEET). The active constituent of the C. obtusa leaves was identified as beta-thujaplicin (C10H12(O2)) by spectroscopic analyses. Responses varied with dose. On the basis of a 24 h LC50 value, acaricidal activity against D. farinaewas more pronounced with beta-thujaplicin (72.2 mg/m2) than benzyl benzoate (89.9 mg/m2) and DEET (377 mg/m2). Acaricidal activity against D. pteronyssinus was more pronounced in beta-thujaplicin (62.1 mg/m2) than benzyl benzoate (72.4 mg/m2) and DEET (193 mg/m2). These results indicate that acaricidal activity of C. obtusa leaves likely results from by beta-thujaplicin. Beta-thujaplicin merits further study as potential house dust mite control agents or lead compounds.

  4. 850nm light-emitting-diode phototherapy plus low-dose tacrolimus (FK-506) as combination therapy in the treatment of Dermatophagoides farinae-induced atopic dermatitis-like skin lesions in NC/Nga mice.

    Science.gov (United States)

    Kim, Chang-Hyun; Cheong, Kyung Ah; Lee, Ai-Young

    2013-11-01

    Light emitting diode (LED) phototherapy is an effective alternative for the treatment of inflammatory skin disorders. Tacrolimus (FK-506) is a potent immunomodulating agent, which has been used to treat AD. Combination therapy is often used in the treatment of AD to improve therapeutic efficacy or to reduce the dose of each drug. To investigate the therapeutic efficacy of monotherapy with either 850nm LED phototherapy or low-dose FK-506, and combination therapy in Dermatophagoides farina (Df)-induced AD-like skin lesions in NC/Nga mice. The Df-induced NC/Nga mice with a clinical score of 7 were used for treatment with LED (10 and 25J/cm(2)) alone, low-dose FK-506 (1mg/kg) or in combination. The synergistic effects of combined therapy were evaluated by dermatitis scores, skin histology, skin barrier function, and immunological parameters, such as IgE, NO, Th2-mediated cytokines and chemokines. Combination therapy with 850nm (25J/cm(2)) LED and low-dose FK-506 showed a significant reduction in the severity of skin lesions. Combined therapy decreased in the serum level of IgE, NO, and in the splenic level of Th2-mediated cytokines and chemokines. Combination therapy significantly also reduced the inflammatory cellular infiltrate into the skin lesions. Moreover, combination therapy led to recovery of skin barrier function in the skin lesions. The use of combination of LED phototherapy and low-dose immunosuppressant improved Df-induced AD-like skin lesions in an NC/Nga mouse model by dominantly reducing IgE, NO, suppressing Th2-mediated immune responses, and inhibiting inflammatory cells, as well as improving skin barrier function. Copyright © 2013 Japanese Society for Investigative Dermatology. Published by Elsevier Ireland Ltd. All rights reserved.

  5. Clinical observation of sublingual dermatophagoides farinae treatment of allergic rhinitis with adenoid hypertrophy in children%舌下含服粉尘螨滴剂治疗儿童变应性鼻炎合并腺样体肥大的临床观察

    Institute of Scientific and Technical Information of China (English)

    谢小娟; 文春秀; 甘金梅; 蒋鸣惊; 莫誉华; 黄跃

    2015-01-01

    Objective:To investigate the clinical effect of sublingual dermatophagoides farinae drops on allergic rhinitis with adenoid hypertrophy in children.Methods:Diagnosis for allergic rhinitis with adenoid hypertrophy of 64 cases were randomly divided into two groups,study group under the tongue with dermatophagoides farinae drops in treatment and control group for the simple routine anti allergy drug treatment were observed for 2 years,changes in VAS scores were recorded at children with nasal allergies such as nasal congestion,nasal itching,sneezing and runny nose and sleep snoring symptoms,by nasal endoscopy to observe the changes of the size of adenoids.Results:The VAS score of nasal allergic symptoms in the study group was significantly decreased (P <0.05)compared with that before treatment.There was significant difference in the VAS score between the two groups after treatment (P<0.05).After treatment,the symptoms such as snoring in the study group were signifiG cantly lower than those in the treatment group (P <0.05),and the symptoms such as snoring and VAS scores were significantly lower than those in the control group (P<0.05);and the two groups of adenoid body size compared with the treatment before the reduction of the difference between the two groups (P<0.05),the comparison between the groups were significantly different (P<0.05).ConcluG sion:The tongue with dermatophagoides farinae drops can effectively control children with allergic rhiG nitis with adenoid hypertrophy of the stuffy nose,nasal itching,sneezing,runny nose,sleep snoring and adenoid body size,as the non operative treatment of allergic rhinitis with adenoid hypertrophy clinical method.%目的::探讨舌下含服粉尘螨滴剂对儿童变应性鼻炎合并腺样体肥大的临床治疗效果.方法:将64例变应性鼻炎合并腺样体肥大患儿随机分为两组,研究组为舌下含服粉尘螨滴剂治疗,对照组为单纯常规抗过敏药物治疗,观察2年,采用 VAS

  6. Cloning, expression, and analysis of the Mal f 6 allergen from Dermatophagoides farinae in China%粉尘螨变应原Der f Mal f 6的克隆、表达及生物信息学分析

    Institute of Scientific and Technical Information of China (English)

    孙金霞; 周鹰; 杨李; 俞黎黎; 王运刚; 卞勇华; 崔玉宝

    2013-01-01

    目的 克隆粉尘螨变应原Der f Mal f 6(简称Mal f 6)编码基因并构建其原核表达体系. 方法 提取粉尘螨总RNA,根据GenBank中的(AY283280.1)序列设计并合成引物,RT-PCR扩增Der f Mal f 6全长基因,克隆至pColdTF DNA载体,转化至E.coli JMI09,取阳性克隆测序;将pCold TF-Mal f 6质粒转化至BL21,IPTG诱导表达,采用SDS-PAGE验证表达产物,采用生物信息学软件预测Mal f 6的理化特性、结构和功能. 结果 RT-PCR获得全长为495 bp的Mal f 6基因.原核表达后经SDS-PAGE电泳显示其全细胞、上清及沉淀物均有蛋白表达,以上清表达量较高.生物信息学分析该蛋白由164个氨基酸组成,分子质量单位为17.7 ku,二级结构由α螺旋(4.88%)、延伸主链(37.8%)和无规则卷曲(57.32%)组成,是亲水性细胞质蛋白,具有肽酰-脯氨酰反式异构酶活性. 结论 粉尘螨变应原Mal f 6原核表达获得成功,为该变应原的进一步研究及规模生产和应用奠定了基础.%Objectives To acquire the cDNA of the Der f Malf 6 gene (Mal f 6) from Dermatophagoides farinae in China and construct a prokaryotic expression system.Methods Total RNA was extracted from D.farinae.The Malf 6 gene was amplified with RT-PCR and cloned into the pCold TF DNA vector.The pCold TF-Mal f 6 was then transformed into E.coli BL21 and induced by IPTG to express the gene.It was then identified with SDS-PAGE.Bioinformatics tools were used to analyze the physiochemical properties,secondary structure,and function of Malf 6.Results The Malf 6 gene was obtained by RT-PCR and was 495 bp in length.The constructed plasmid pCold TF-Mal f 6 was then transformed into E.coli BL21 and induced to express the gene.SDS-PAGE detected a specific band in whole cells,supernatant,and precipitate containing pCold TF-Mal f 6.Bioinformatics indicated that the allergen consisted of 164 amino acids and had a molecular weight of 17.7 KDa.Its secondary structure consisted of an alpha helix (4.88

  7. 舌下含服粉尘螨滴剂对小儿过敏性鼻炎伴腺样体肥大的疗效%Specific Sublingual Immunotherapy with Dermatophagoides Farinae Drops for Allergic Rhinitis Complicated by Adenoid Hypertrophy in Children

    Institute of Scientific and Technical Information of China (English)

    吕显林; 岳耀光; 张永强; 朱绪亮; 张俊; 郭军; 黄丽芳; 梁仕才

    2015-01-01

    ABSTRACT:Objective To investigate the curative effects of specific sublingual immunotherapy with Dermatophagoides farinae drops on allergic rhinitis complicated by adenoid hypertrophy in children.Methods Ninety children with allergic rhinitis and adenoid hypertrophy randomly re-ceived sublingual immunotherapy with Dermatophagoides farinae drops(group A,n=30),conven-tional treatment with anti-allergic drugs(group B,n=30),or their combination(group C,n=30). Nasal symptoms(nasal congestion,nasal itching,sneezing,runny nose,etc.)and snoring symp-toms(labored breathing,waking up repeatedly,snoring,mouth breathing,etc.)were evaluated u-sing the visual analogue scale(VAS)before and after treatment for 6 months.The maximum thickness of adenoid/anterior-posterior diameter of nasopharyngeal air space (A/N)ratio was measured by X-ray absorptiometry and computed tomography.Results Before treatment,there were no significant differences among the three groups in VAS scores of nasal symptoms and sno-ring symptoms,as well as in A/N ratio(P >0.05).After treatment for 6 months,VAS scores of nasal symptoms and snoring symptoms and A/N ratio obviously reduced in all the three groups (P 0.05).Compared with group B,VAS scores of snoring symp-toms and A/N ratio increased in group A but decreased in group C(P <0.05).Conclusion There is a close relationship between allergic rhinitis and adenoid hypertrophy in children.Specific sub-lingual immunotherapy not only improves nasal allergic symptoms,but also relieves snoring symptoms through reducing adenoid volume.Therefore,specific sublingual immunotherapy pro-vides a new method for non-surgical treatment of allergic rhinitis complicated by adenoid hyper-trophy.%目的:探讨儿童过敏性鼻炎伴腺样体肥大患儿采用舌下含服粉尘螨滴剂治疗的疗效。方法将90例过敏性鼻炎合并腺样体肥大的患儿按随机数字表法分为 A、B、C 3组,每组30例。A 组给予舌下含服粉尘螨滴剂治疗;B 组

  8. 舌下含服粉尘螨滴剂治疗支气管哮喘伴变应性鼻炎的疗效%Efficacy of Specific Sublingual Immunotherapy with Dermatophagoides Farinae Drops on Bronchitic Asthma and Allergic Rhinitis in Children

    Institute of Scientific and Technical Information of China (English)

    谢庆玲; 甄宏; 谭颖; 温志红; 胡琼燕; 李东云; 李柳青; 梁珍花

    2011-01-01

    Objective To explore the efficacy and safety of specific sublingual immunotherapy with dermatophagoides farinae drops on bronchitic asthma and allergic rhinitis in children. Methods Five hundred and sixteen children aged 4 - 13 years old with asthma and allergic rhinitis were involved in the study. Among them, 291 cases had received specific sublingual immunotherapy for 1 year( immunotherapy group) and the other (225 cases) had not received specific sublingual immunotherapy as control group. Skin prick test with 10 common allergens were taken in all subjects and positive reaction to dermatophagoides was confirmed. Immunotherapy group were assigned into 4 subgroups according to the results of skin prick test:only sensitized to mites group(80 cases), sensitized to mites and cockroach group(71 cases), sensitized to mites and pollen group(74 cases) ,sensitized to mites and dog hair group(66 cases). Dermatophagoides farinae drops(DFD) was used for sublingual immunotherapy. Asthma control questionnaire (ACQ) scores, rhinitis symptoms scores, drug use before and after treatment and side reaction were recorded. Results 1. After 12 months treatment of sublingual immunotherapy ,the ACQ scores in immunotherapy group and control group were 0. 28 ± 0. 33 and 1.07 ± 0.68, and they were decreased separately by (74.03 ± 37.66 ) % and ( 29.32 ± 44.53 ) % compared with those before treatment ( 1.76 ±0. 75 and 1.55 ±0.62 ) ,which showed extremely significant decreased ACQ grading in the irnmunotherapy patients ( Z = - 154. 109 ,P < 0.000 1 ). 2. After 12 months treatment of sublingual immunotherapy, the rhinitis symptoms scores in immunotherapy group and control group were 0. 337 ± 0.479 and 0. 560 ± 0. 634, and they were decreased by 70.8% and 39.1% compared with those before treatment (0. 899 ± 0.667 and 0. 892 ± 0. 688 ), and there was significant difference between both groups (x2 =51. 949,P < 0. 000 1 ). 3. After 12 months treatment of sublingual

  9. 屋尘螨1类变应原T细胞表位融合肽对过敏性哮喘小鼠的免疫治疗效果%Immunotherapeutic Effect of Dermatophagoides pteronyssinus Group 1 Allergen T Cell Epitope Peptide Against Allergic Asthma in Mice

    Institute of Scientific and Technical Information of China (English)

    李朝品; 赵蓓蓓; 湛孝东

    2016-01-01

    目的 探讨以屋尘螨(Dermatophagoides pteronyssinus)1类变应原T细胞表位融合肽(TAT-IhC-DPTCE)为疫苗,评价其对过敏性哮喘小鼠特异性免疫治疗的效果. 方法 120只SPF级BALB/c小鼠随机均分为PBS组(阴性对照,A组)、ProDer p 1变应原致敏组(B组)、ProDer p 1变应原免疫治疗组(C组)、DPTCE蛋白免疫治疗组(D组)、TAT-DPTCE蛋白免疫治疗组(E组)和TAT-IhC-DPTCE蛋白免疫治疗组(F组),每组20只.分别于第0、7、14天,A组小鼠腹腔注射PBS,B~F组小鼠均腹腔注射屋尘螨变应原提取液10 μg.第21天起,A组小鼠雾化吸入PBS,B~F组小鼠均吸人0.5 μg/ml屋尘螨变应原提取液,1次/d×30 min,连续7d.C~F组小鼠于第25 ~27天雾化前30 min分别腹腔注射100 μg/ml ProDer p 1、DPTCE、TAT-DPTCE和TAT-IhC-DPTCE溶液各200μl,进行特异性免疫治疗,A、B组小鼠分别注射200 μlPBS.最后1次雾化后24 h,处死各组小鼠.HE染色观察小鼠肺组织病理变化.分别收集各组20只小鼠支气管肺泡灌洗液(BALF),ELISA检测BALF中γ干扰素(IFN-γ)、白细胞介素13(IL-13)、IL-10和β转化生长因子(TGF-β)的水平,并计数嗜酸粒细胞数量(EOS).分别取各组5只小鼠眼眶血,ELISA检测血清中变应原特异性IgE、IgG1和IgG.的抗体水平.结果 HE染色镜检结果显示,与B组比较,F组小鼠支气管周围嗜酸粒细胞增多、上皮细胞脱落和支气管上皮细胞肥大等肺部炎症明显减轻.小鼠BALF中,F组的IFN-γ水平为(298.75±26.09) pg/ml,高于B组(158.71±20.89) pg/ml、C组(210.38±18.92) pg/ml、D组(229.44±13.00) pg/ml和E组(233.24±20.39) pg/ml(P<0.01);IL-10和TGF-β水平与IFN-γ相似,F组IL-10和TGF-β水平分别为(105.32±.7.24)和(119±9.33) pg/ml,均高于B组(23.29±3.18)和(41.19±4.63) pg/ml、C组(43.54±4.28)和(60.19±6.47) pg/ml、D组(51.33±6.19)和(69.34±8.27) pg/ml、E组(52.78±7.83)和(71.22±7.94) pg/ml (P<0.01);而C、D、E和F组的IL-13水平分别为(47

  10. 重组纳米级粉尘螨变应原第1组分的制备及其在哮喘小鼠中的作用研究%The preparation of recombinant Nanoscale Der f 1 allergen of Dermatophagoides farinae and its roles in mouse asthma

    Institute of Scientific and Technical Information of China (English)

    马桂芳; 周鹰; 王运刚; 杨李; 崔玉宝

    2012-01-01

    The current study is aimed to develop a genetic method for recombinant allergen on nanometer levels. The gene sllB coding surface layer protein (S-layer) was truncated with 600 bp at the 3' end, and then linked with the dust mite allergen Der f 1 at the 3' end and 5' with Strep-tag I to get a cloning plasmid pMD19- T-Strep-tag 1/sLLB1_2 7O6bI/Der f 1. After being verified by sequencing, the expression plasmid pET28a(+)-Strep-tag VsllB, _2 706 bP/Der f 1 was transformed into E. Coli BL21 and expressed with IPTG induction. By transmission electron microscopy, the expression product rDer f 1/S-layer was located on the surface of the engineering strain with avaerage diameter of (116.43 ±14) nm, which demonstrated that the expression system was developed successfully for recombinant nanometer-scale allergen. Then the recombinant allergen rDer f 1 and rDer f 1/S-layer was used to treat the asthmatic mouse model. The results from the experimental animals suggested that the two recombinant allergens can alleviate the lung inflammation, decrease the total cell numbers of bronchoalveolar lavage fluid, the numbers of neutrophils and eosinophils, and the levels of total IgE, the mite-specific IgE, the total IgG, mite-specific IgG in BALF, increase the levels of Thl cytokines, and decrease the levels of Th2 cytokines. The therapeutic effect and immunoregulatory roles of the recombinant nano-level allergen rDer f 1/S-layer was better than that of the recombinant allergen rDer f 1. In brief, the recombinant product of the group 1 allergen of Dermatophagoides farinae is obtained on nanometer level, which can induce immune tolerance and have good immunogenicity.%目的 应用基因工程技术获得纳米级粉尘螨变应原第1组分s-layer/Der f 1,建立重组纳米级变应原的方法.方法 利用S-layer/Strep-tag Ⅰ这一具有自我组装能力的纳米模式母体嵌合粉尘螨变应原第1组分,并置大肠杆菌中表达,用分离纯化获得的表达产物免疫治

  11. Construction and expression of a chimeric gene with T-/B-cell epitopes of major allergen group 1 from Dermatophagoides farina%粉尘螨1类变应原T和B细胞表位嵌合基因的构建与表达

    Institute of Scientific and Technical Information of China (English)

    徐海丰; 徐朋飞; 王克霞; 李朝品

    2014-01-01

    目的:构建粉尘螨1类变应原(Der f 1)的T和B细胞表位嵌合基因原核表达载体。方法将Der f 1包含的5个T细胞表位(T1~T5)和6个B细胞表位(B1~B6),以B1-T1-B2-T2-B3-T3-B4-T4-B5-T5-B6和B1-B2-B3-B4-B5-B6-T1-T2-T3-T4-T5连接方式直接合成表位嵌合基因,并分别命名为Der f 1A和Der f 1B。构建原核表达重组质粒pET-28a(+)-Der f 1A和pET-28a(+)-Der f 1B,双酶切及测序验证的阳性克隆转化到E.coli BL21(DE3)后诱导表达。SDS-PAGE分析表达产物后进行纯化,并进行Western blotting鉴定。ELISA法检测嵌合蛋白对粉尘螨过敏患者血清IgE抗体的结合力。结果双酶切及测序结果表明,成功构建了原核表达重组质粒pET-28a(+)-Der f 1A和pET-28a(+)-Der f 1B;SDS-PAGE分析表明,Der f 1A和Der f 1B诱导并纯化成功,Western blotting结果进一步证实纯化了Der f 1A和Der f 1B嵌合蛋白。与Der f 1相比,Der f 1A和Der f 1B嵌合蛋白与粉尘螨过敏患者血清IgE抗体的结合能力显著降低(P均<0.05),但Der f 1A和Der f 1B间差异无统计学意义(P>0.05)。结论成功表达了Der f 1的T和B细胞表位嵌合蛋白,为粉尘螨过敏的特异性免疫治疗奠定了基础。%Objective To construct and express a chimeric gene with T-/B-cell epitopes of the major allergen group 1 from Dermatophagoides farina(Der f 1). Methods Two chimeric genes,Der f 1A and Der f 1B,were synthesized as B1-T1-B2-T2-B3-T3-B4-T4-B5-T5-B6 and B1-B2-B3-B4-B5-B6-T1-T2-T3-T4-T5 pattens. Two recombinant vectors,pET-28a(+)-Der f 1A and pET-28a(+)-Der f 1B,were constructed for prokaryotic expression. These chimeric genes were induced by 1 mmol/L IPTG (final concentration),digested with restriction enzymes and sequenced. The chimeric proteins were analyzed by SDS-PAGE and Western blotting. Results After digestion by restriction enzymes and sequencing,the recombinant vectors were constructed successfully. The

  12. Isolation and characterization of the 68 kD allergen from house dust mite Dermatophagoides pteronyssinus

    Directory of Open Access Journals (Sweden)

    KATARINA MILOVANOVIĆ

    2009-05-01

    Full Text Available House dust mites (HDM represent a major source of allergens, contributing to the increasing incidence of type I hypersensitivity disease worldwide. Over 30 different IgE-binding proteins from the HDM extract were detected. Although group 1 and 2 have been identified as major allergens, due to the safety and efficacy of allergy diagnosis and immunotherapy, there is a need to carefully evaluate the clinical relevance of other allergens present in the HDM extract. In regard to this, a high molecular mass allergen of about 68 kD was purified from the HDM extract using a combination of gel permeation chromatography and reversed-phase chromatography. The IgG and IgE reactivity of the purified protein were preserved during the purification process, as confirmed by Western blot analysis with polyclonal rabbit antibodies and dot blot analysis with a pool of sera from subjects with house dust mite allergy, respectively. In addition, the IgE reactivity was confirmed using ELISA testing with nine patient sera. The biological potency of the 68 kD allergen was confirmed by skin prick testing in five allergic subjects, suggesting that the high molecular mass allergen is a good candidate for component-resolved diagnosis of house dust mite allergy and eventual therapeutic treatment.

  13. Patch testing with dermatophagoides and its correlation with chronic eczema and atopic dermatitis

    Directory of Open Access Journals (Sweden)

    Kapur Chetna

    2009-01-01

    Full Text Available Background: Chronic eczema is commonly encountered in the Indian set up. So also is atopic dermatitis. House dust mites (Dermatophagoides are implicated in various diseases like atopic dermatitis, asthma, and perennial rhinitis. It has also been proven that patch testing with Dermatophagoides pteronyssinus (DP is important for detection of contact sensitization in chronic dermatitis. Aims: To study clinical characteristics of DP mix positive patients with regards to chronic dermatitis and atopic dermatitis. Methods: Dermatology outpatients presenting to the department of Skin and STD of Kasturba Medical College (KMC, with clinically diagnosed atopic dermatitis and chronic eczema were chosen for the study. Inclusion and exclusion criteria were well demarked. Eighty six randomly selected patients of dermatitis were subjected to patch testing with standard series and DP mix. Results: Of the 86, 50 (58% showed positive reaction to DP mix. Among these positive patients, chronic dermatitis was seen in 42 (84% with involvement of exposed parts in 37 (74%. Atopic dermatitis was seen in 19 patients (38% from DP positive group whereas it was observed in 4 patients (17% from the other group. Conclusion: Dermatophagoides mix positivity was statistically significant in chronic eczema as well as atopic dermatitis. Patch testing is an important tool to detect delayed type allergy to house dust mite.

  14. ALLERGIC ASTHMA INDUCED IN RHESUS MONKEYS BY HOUSE DUST MITE (DERMATOPHAGOIDES FARINAE). (R826246)

    Science.gov (United States)

    The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Concl...

  15. Gene expression in the skin of dogs sensitized to the house dust mite Dermatophagoides farinae.

    Science.gov (United States)

    Schamber, Paz; Schwab-Richards, Rachel; Bauersachs, Stefan; Mueller, Ralf S

    2014-10-01

    Atopic dermatitis is a multifactorial allergic skin disease in humans and dogs. Genetic predisposition, immunologic hyperreactivity, a defective skin barrier, and environmental factors play a role in its pathogenesis. The aim of this study was to analyze gene expression in the skin of dogs sensitized to house dust mite antigens. Skin biopsy samples were collected from six sensitized and six nonsensitized Beagle dogs before and 6 hr and 24 hr after challenge using skin patches with allergen or saline as a negative control. Transcriptome analysis was performed by the use of DNA microarrays and expression of selected genes was validated by quantitative real-time RT-PCR. Expression data were compared between groups (unpaired design). After 24 hr, 597 differentially expressed genes were detected, 361 with higher and 226 with lower mRNA concentrations in allergen-treated skin of sensitized dogs compared with their saline-treated skin and compared with the control specimens. Functional annotation clustering and pathway- and co-citation analysis showed that the genes with increased expression were involved in inflammation, wound healing, and immune response. In contrast, genes with decreased expression in sensitized dogs were associated with differentiation and barrier function of the skin. Because the sensitized dogs did not show differences in the untreated skin compared with controls, inflammation after allergen patch test probably led to a decrease in the expression of genes important for barrier formation. Our results further confirm the similar pathophysiology of human and canine atopic dermatitis and revealed genes previously not known to be involved in canine atopic dermatitis.

  16. Evidence refuting the contribution of the fungus Aspergillus penicillioides to the allergenicity of the house dust mite Dermatophagoides pteronyssinus.

    Science.gov (United States)

    Hay, D B; Hart, B J; Douglas, A E

    1992-01-01

    This communication demonstrates unequivocally that the fungi associated with house dust mites do not contribute to mite allergenicity. The evidence is twofold: first, larval mites which lack fungi have allergen profiles indistinguishable from fungus-bearing adult mites. Second, the allergen profile of experimentally-derived fungus-free adult mites and mites re-fed the fungus Aspergillus penicillioides are identical.

  17. 486 Therapeutic Effect and Security in Asthmatics Adult Patients Treated with Dermatophagoides Pteronyssinus Allergen Sublingual Immunotherapy

    Science.gov (United States)

    Rodríguez, José; Castro, Raúl; Labrada, Alexis; Alvarez, Mirta; Ronquillo, Mercedes; González, Mayda; Navarro, Bárbara; Mateo, Maytee; Oliva, Yunia; García, Iris; Enriquez, Irene

    2012-01-01

    Background The specific active immunotherapy, employing vaccine of allergen of mite is a treatment considered as effective for the respiratory allergy and asthma. The sublingual route has minor risk of systematises reactions. The objective of this study was to determine the therapeutic effect and security of sublingual immunotherapy (ITSL) employing the standard vaccine VALERGEN-DP (BIOCEN, CUBA) in a population of asthmatic Cuban patients. Methods A phase II Clinical Trials double blind, placebo controlled in a total of 40 adult patients with mild or moderate asthma and specific sensibility preponderant to this mite. Half of patients received drops by sublingual route with growing doses up to 2000 UB. Results The treatment was effective in the reduction of clinical symptoms and medication intake as compared to conventional treatment in control group. The cutaneous sensibility to this mite was significant reduced, increasing in 1.9 log; the amount of necessary allergen to provoke a positive Prick Test. An improvement of the lung function was observed with a significant reduction (P < 0.05) of expiratory pick flow variability. The frequency of local reactions were only 0.58% of administration. Conclusions The VALERGEN-DP vaccine is an effective treatment and profitable against asthma in our population and guarantee its generalization in the Allergy Services of our health system.

  18. Acaricidal activity of Cymbopogon citratus and Azadirachta indica against house dust mites

    Institute of Scientific and Technical Information of China (English)

    Azima Laili Hanifah; Siti Hazar Awang; Ho Tze Ming; Suhaili Zainal Abidin; Maizatul Hashima Omar

    2011-01-01

    Objective: To examine the acaricidal effects of the essential oil of Cymbopogon citratus leaf extract (lemongrass) and ethanolic Azadirachta indica leaf extract (neem) against house dust mites Dermatophagoides farinae (D. farinae) and Dermatophagoides pteronyssinus (D. pteronyssinus). Methods: Twenty-five adults mites were placed onto treated filter paper that is soaked with plant extract and been tested at different concentrations (50.00%, 25.00%, 12.50%, 6.25% and 3.13%) and exposure times (24hrs, 48hrs, 72hrs and 96 hrs). All treatments were replicated 7 times, and the experiment repeated once. The topical and contact activities of the two herbs were investigated.Results:Mortalities from lemongrass extract were higher than neem for both topical and contact activities. At 50 % concentration, both 24 hrs topical and contact exposures to lemongrass resulted in more than 91% mortalities for both species of mites. At the same concentration and exposure time, neem resulted in topical mortalities of 40.3% and 15.7% against D. pteronyssinus and D.farinae respectively; contact mortalities were 8.0% and 8.9% against the 2 mites, respectively. There was no difference in topical mortalities of D. pteronyssinus from exposure to concentrations of lemongrass and neem up to 12.50%; lemongrass was more effective than neem at the higher concentrations. Conclusions: Generally, topical mortalities of D. farinae due to lemongrass are higher than that due to neem. Contact mortalities of lemongrass are always higher that neem against both species of mites.

  19. Detection of B-lymphocytes secreting antibodies to Dermatophagoides antigens

    DEFF Research Database (Denmark)

    Sparholt, S H; Barington, T; Schou, C

    1991-01-01

    An enzyme-linked immunospot assay (ELI-spot assay) has been established to count individual cells secreting antibodies to Dermatophagoides spp. allergens. Initial optimization of the assay was performed using Der p I-specific murine hybridoma cell lines. Inhibition with soluble purified allergen ...

  20. Identification and characterization of Sarcoptes scabiei and Dermatophagoides pteronyssinus glutathione S-transferases: implication as a potential major allergen in crusted scabies.

    Science.gov (United States)

    Dougall, Annette; Holt, Deborah C; Fischer, Katja; Currie, Bart J; Kemp, David J; Walton, Shelley F

    2005-11-01

    The astigmatid mite Sarcoptes scabiei is the causative agent of scabies, a highly infectious parasitic disease of the skin. Although the mite causes marked hypersensitivity reactions, particularly in crusted (severe) scabies, little is known about the specific scabies mite molecules involved in such immunologic responses. We have identified six genes encoding scabies mite homologues of mu and delta-like glutathione S-transferases (GSTs) as well as novel house dust mite GSTs. A mu class S. scabiei GST was subcloned into a prokaryotic expression system. The purified recombinant protein rSsGST01 reacted strongly with IgE and IgG4 in sera from crusted scabies patients. This response was not observed with control antigens or with ordinary scabies and uninfested patient sera. In addition, the specific IgE response to rSsGST01 did not correlate with the total IgE level of the patient. These results suggest that GST may play a role in the pathophysiology associated with crusted scabies.

  1. [Mites allergy in children from Tula region].

    Science.gov (United States)

    Zlobina, Zh M; Pron'kina, O V; Khlgatian, S V; Berzhets, A I; Berzhets, V M

    2006-01-01

    Characteristics of allergy to mites in children living in Tula region have been revealed. It was shown that mites from Pyroglyphidae (Dermatophagoides pteronyssinus, Dermatophagoides farinae), Cheyletidae and Glycyphagidae (G. destructor, G. domesticus) families play important role in development of atopic allergy in children from this region. Efficacy of plant-origin acaricide "Milbiol" as part of prophylactic measures was evaluated. Its use in children with mild and intermediate asthma led to decrease of number of wheezing episodes, improvement of respiratory function, lessening of clinical signs of allergic rhinitis, decrease or discontinuation of usage of vasoconstrictive preparations.

  2. Određivanje alergena grinja u kućnoj prašini enzim-imunokemijskom metodom

    OpenAIRE

    Prester, Ljerka; Brčić Karačonji, Irena; Macan, Jelena

    2007-01-01

    Cilj ovoga rada bio je odrediti razine alergena grinja Dermatophagoides pteronyssinus (Der p 1) i Dermatophagoides farinae (Der f 1) u 30 uzoraka kućne prašine uzorkovane u gradskim stanovima u Zagrebu enzim-imunokemijskom metodom (ELISA, Indoor Biotechnologies Ltd, Carfiff, UK). Metoda se koristi tehnikom dvaju monoklonskih protutijela, a primjenjuje se kao standardna metoda za određivanje alergena grinja u prašini. Uzorci prašine skupljeni su usisavanjem prašine s tepiha u celulozne filtre....

  3. Review, comments and perspectives with regard to Renata Sõukand's doctoral dissertation Herbal landscape / Almo Farina

    Index Scriptorium Estoniae

    Farina, Almo

    2011-01-01

    9. nov. 2010 kaitses Tartu Ülikoolis doktoriväitekirja semiootika ja kulturoloogia doktorant Renata Sõukand, juhendaja Kalevi Kull (Tartu Ülikool), oponendid Almo Farina (Urbino Ülikool, Itaalia) ja Myrdene Anderson (Purdue Ülikool, USA Indiana)

  4. Renata Sõukandi väitekiri Ravimtaimemaastik [Herbal landscape] / Almo Farina

    Index Scriptorium Estoniae

    Farina, Almo

    2011-01-01

    9. nov. 2010 kaitses Tartu Ülikoolis doktoriväitekirja semiootika ja kulturoloogia doktorant Renata Sõukand, juhendaja Kalevi Kull (Tartu Ülikool), oponendid Almo Farina (Urbino Ülikool, Itaalia) ja Myrdene Anderson (Purdue Ülikool, USA Indiana)

  5. Review, comments and perspectives with regard to Renata Sõukand's doctoral dissertation Herbal landscape / Almo Farina

    Index Scriptorium Estoniae

    Farina, Almo

    2011-01-01

    9. nov. 2010 kaitses Tartu Ülikoolis doktoriväitekirja semiootika ja kulturoloogia doktorant Renata Sõukand, juhendaja Kalevi Kull (Tartu Ülikool), oponendid Almo Farina (Urbino Ülikool, Itaalia) ja Myrdene Anderson (Purdue Ülikool, USA Indiana)

  6. Renata Sõukandi väitekiri Ravimtaimemaastik [Herbal landscape] / Almo Farina

    Index Scriptorium Estoniae

    Farina, Almo

    2011-01-01

    9. nov. 2010 kaitses Tartu Ülikoolis doktoriväitekirja semiootika ja kulturoloogia doktorant Renata Sõukand, juhendaja Kalevi Kull (Tartu Ülikool), oponendid Almo Farina (Urbino Ülikool, Itaalia) ja Myrdene Anderson (Purdue Ülikool, USA Indiana)

  7. 粉尘螨Der f2变应原的分离纯化及其特征%Purification and Characterization of Der f2 Allergen in Dermatophagoides f arinae

    Institute of Scientific and Technical Information of China (English)

    张鲁雁; 张剑霞; 江世益; 温廷桓; 项黎

    2001-01-01

    Purpose To purify Der f2 allergen from the spent culture medium extract of Dermatophagoides farinae mite(Dff). Methods We purified Der f2 with the aid of Sephadex G-100 gel filtration, DEAE-ion exchange chromatography and polyacrylamide gel electrophoresis, detected the molecular weight of Der f2 using SDS-PAGE method,and analysed the thermostability of Der f2 by heating at 100℃ for 15 min. Results The pure Der f2 was obtained from Dff. The molecular weight of Der f2 was 14 000.The heated-treated Der f2 retained 50% ~83.3% of the activity of the native allergen. Conclusions The properties of purified allergen coincided with the characterization of Der f2.%目的用粉尘螨(Dermatophagoides farnae)代谢培养基浸液(Dff)分离纯化粉尘螨2类变应原(Der f2)。方法采用Sephadex G-100层析、DEAE离子交换层析、PAGE等方法分离纯化Der f2,并对Der f2作 SDS-PAGE测定和热稳定性测定。结果从Dff分离纯化得到较纯的Der f2,经SDS-PAGE测定其相对分子质 量为14 000,并经100℃加热15 min后,仍保留50%~83.3%生物学活性。结论从Dff中分离纯化的变应原, 其物化性质符合Der f2特征。

  8. Sensitization Rates for Various Allergens in Children with Allergic Rhinitis in Qingdao, China

    Directory of Open Access Journals (Sweden)

    Hang Lin

    2015-09-01

    Full Text Available The aim of this study was to determine the prevalence of sensitization to common allergens in children with allergic rhinitis (AR living in Qingdao, China. We conducted a retrospective analysis for AR cases, who underwent skin prick tests (SPT in Qingdao. A total of 2841 children with AR qualified for the inclusion criteria (Age 3–5 years: 1500 children; Age 6–12 years: 1341 children. The most common inhaled allergens to which the AR children were sensitive were Dermatophagoides pteronyssinus (69.3%, Dermatophagoides farinae (66.2% and mould 1 (Penicillium notatum 38.9%; while the corresponding ingested allergens were mussel (39.2%, shrimp (36.3% and carp (36.5%. The prevalence of sensitization to inhaled allergens and food allergens was higher in children >6 years of age as compared to that in children 3–5 years of age (all p < 0.05. Children >6 years old were more sensitive to dust mite as compared to children 3–5 years old (p < 0.05. Sensitization to dust mite was more common in males than in females (p = 0.05. In this study, Dermatophagoides pteronyssinus and Dermatophagoides farinae were the most common allergens causing AR in children in Qingdao, China. Older children with AR, particularly males, were found to be more sensitive to dust mite.

  9. Ácaros em poeira domiciliar das capitais brasileiras e ilha Fernando de Noronha

    Directory of Open Access Journals (Sweden)

    Archibaldo Bello Galvão

    1986-12-01

    Full Text Available O presente trabalho é um estudo taxinômico de ácaros admitidos ou não como alergizantes, encontrados em amostras de poeira domiciliar, colhidas em todas as capitais do Brasil e Território Fernando de Noronha por guardas da SUCAM e enviadas por essa entidade ao Professor Leonidas de Mello Deane. Foram classificadas dez espécies pertencentes a cinco famílias, cujas posições sistemáticas são determinadas por definições e chaves: Pyroglyphus africanus, Euroglyphus maynei, Dermatophagoides pteronyssinus, Dermatophagoides farinae, Dermatophagoides deanei, Sturnophagoides brasiliensis, Blomia tropicalis, Suidasia pontifica, Chortoglyphus arcuatus e Cheyletus malaccensis.This paper deals with the geographical distribution of astigmatid mites related to human allergy and found in house dust samples collected in all states capitals in Brazil. Definitions and keys for the identification of the species of mites are presented.

  10. Comparison of the skin-prick test and Phadia ImmunoCAP as tools to diagnose house-dust mite allergy.

    Science.gov (United States)

    Jung, Yong Gi; Cho, Hyun-Jin; Park, Ga Young; Min, Jin-Young; Kim, Hyo Yeol; Dhong, Hun-Jong; Chung, Seung Kyu; Kim, Seon Woo

    2010-01-01

    When the skin-prick test (SPT) and in vitro test such as ImmunoCAP assay are performed simultaneously, results do not always coincide in some patients. Our objectives, therefore, were (1) to assess differences in allergic test results according to age group and (2) to establish appropriate guidelines for diagnosing mite allergy according to age. A total of 692 participants complaining of allergic rhinitis symptoms participated. Patients were divided according to age; the mean age was 32 years (range, 8-76 years). The SPT and ImmunoCAP assays were performed to detect allergies to house-dust mites (Dermatophagoides pteronyssinus and Dermatophagoides farinae). The association between age and the result of each allergy test were examined, and a cutoff age for proper application of each test was than estimated. Three hundred thirty-six patients (48.6%) were allergic to D. pteronyssinus and 350 patients (50.6%) were allergic to D. farinae. In the case of D. pteronyssinus, SPT was proved to be more useful in detecting allergy for subjects 30 years old (p allergy tests according to age using true allergens. For patients >50 years of age, the ImmunoCAP was found to be the preferred method for detecting allergy to house-dust mites and for patients <30 years old, SPT is the recommended first choice.

  11. Cloning, sequence analysis, and expression of cDNA coding for the major house dust mite allergen, Der f 1, in Escherichia coli

    Directory of Open Access Journals (Sweden)

    Y. Cui

    2008-05-01

    Full Text Available Our objective was to clone, express and characterize adult Dermatophagoides farinae group 1 (Der f 1 allergens to further produce recombinant allergens for future clinical applications in order to eliminate side reactions from crude extracts of mites. Based on GenBank data, we designed primers and amplified the cDNA fragment coding for Der f 1 by nested-PCR. After purification and recovery, the cDNA fragment was cloned into the pMD19-T vector. The fragment was then sequenced, subcloned into the plasmid pET28a(+, expressed in Escherichia coli BL21 and identified by Western blotting. The cDNA coding for Der f 1 was cloned, sequenced and expressed successfully. Sequence analysis showed the presence of an open reading frame containing 966 bp that encodes a protein of 321 amino acids. Interestingly, homology analysis showed that the Der p 1 shared more than 87% identity in amino acid sequence with Eur m 1 but only 80% with Der f 1. Furthermore, phylogenetic analyses suggested that D. pteronyssinus was evolutionarily closer to Euroglyphus maynei than to D. farinae, even though D. pteronyssinus and D. farinae belong to the same Dermatophagoides genus. A total of three cysteine peptidase active sites were found in the predicted amino acid sequence, including 127-138 (QGGCGSCWAFSG, 267-277 (NYHAVNIVGYG and 284-303 (YWIVRNSWDTTWGDSGYGYF. Moreover, secondary structure analysis revealed that Der f 1 contained an a helix (33.96%, an extended strand (17.13%, a ß turn (5.61%, and a random coil (43.30%. A simple three-dimensional model of this protein was constructed using a Swiss-model server. The cDNA coding for Der f 1 was cloned, sequenced and expressed successfully. Alignment and phylogenetic analysis suggests that D. pteronyssinus is evolutionarily more similar to E. maynei than to D. farinae.

  12. Immunoinformatics and Similarity Analysis of House Dust Mite Tropomyosin

    Directory of Open Access Journals (Sweden)

    Mohammad Mehdi Ranjbar

    2015-10-01

    Full Text Available Background: Dermatophagoides farinae and Dermatophagoides pteronyssinus are house dust mites (HDM that they cause severe asthma and allergic symptoms. Tropomyosin protein plays an important role in mentioned immune and allergic reactions to HDMs. Here, tropomyosin protein from Dermatophagoides spp. was comprehensively screened in silico for its allergenicity, antigenicity and similarity/conservation.Materials and Methods: The amino acid sequences of D. farinae tropomyosin, D. pteronyssinus and other mites were retrieved. We included alignments and evaluated conserved/ variable regions along sequences, constructed their phylogenetic tree and estimated overall mean distances. Then, followed by with prediction of linear B-cell epitope based on different approaches, and besides in-silico evaluation of IgE epitopes allergenicity (by SVMc, IgE epitope, ARPs BLAST, MAST and hybrid method. Finally, comparative analysis of results by different approaches was made.Results: Alignment results revealed near complete identity between D. farina and D. pteronyssinus members, and also there was close similarity among Dermatophagoides spp. Most of the variations among mites' tropomyosin were approximately located at amino acids 23 to 80, 108 to 120, 142 to 153 and 220 to 230. Topology of tree showed close relationships among mites in tropomyosin protein sequence, although their sequences in D. farina, D. pteronyssinus and Psoroptes ovis are more similar to each other and clustered. Dermanyssus gallinae (AC: Q2WBI0 has less relationship to other mites, being located in a separate branch. Hydrophilicity and flexibility plots revealed that many parts of this protein have potential to be hydrophilic and flexible. Surface accessibility represented 7 different epitopes. Beta-turns in this protein are with high probability in the middle part and its two terminals. Kolaskar and Tongaonkar method analysis represented 11 immunogenic epitopes between amino acids 7-16. From

  13. Enzymatic activities of allergen extracts from three species of dust mites and cockroaches commonly found in Korean home.

    Science.gov (United States)

    Jeong, Kyoung Yong; Kim, Chungryul; Yong, Tai-Soon

    2010-06-01

    Allergen extracts from dust mites and cockroaches commonly found in Korean homes were used to evaluate their enzymatic activity as they are believed to influence allergenicity. Allergen extracts were prepared from 3 dust mite species (Dermatophagoides farinae, D. pteronyssinus, and Tyrophagus putrescentiae) and 3 cockroach species (Blattella germanica, Periplaneta americana, and P. fuliginosa) maintained in the Korea National Arthropods of Medical Importance Resource Bank. Proteins were extracted in PBS after homogenization using liquid nitrogen. The activities of various enzymes were investigated using the API Zym system. No significant difference in phosphatase, lipase, or glycosidase activity was observed among the 6 allergen extracts, but much difference was observed in protease activity. Protease activity was assessed in more detail by gelatin zymography and the EnzChek assay. Extract from T. putrescentiae showed the highest protease activity, followed by those of the cockroach extracts. Extracts from D. farinae and D. pteronyssinus showed only weak protease activity. Gelatinolytic activity was detected mainly in a 30-kDa protein in D. farinae, a 28-kDa protein in D. pteronyssinus, a > 26-kDa protein in T. putrescentiae, a > 20-kDa protein in B. germanica, and a > 23-kDa protein in P. americana and P. fuliginosa. The information on various enzymatic activities obtained in this study may be useful for future studies. In particular, the strong protease activity found in cockroach extracts could contribute to sensitization to cockroach allergens, which is known to be associated with the development of asthma.

  14. Prevalencia de sensibilización a aeroalérgenos en pacientes con rinitis alérgica en el sur de Bolivia

    Directory of Open Access Journals (Sweden)

    Eliot Iván Narváez-Gómez

    2016-08-01

    Full Text Available La rinitis alérgica es la enfermedad crónica más común, de elevado impacto sanitario y de importancia creciente en la mayor parte del mundo, los aeroalérgenos de ácaros, hongos y pólenes constituyen los desencadenantes más frecuentes de alergia respiratoria, por lo que se realizó un estudio de prevalencia para comprobar la sensibilización cutánea a los mismos, utilizando la prueba de Prick en pacientes con rinitis alérgica en el sur de Bolivia. El estudio fue descriptivo en el universo de pacientes derivados al servicio de Alergia en la ciudad de Tarija. Se incluyeron 350 pacientes con diagnóstico de rinitis alérgica entre 11 y 60 años y se realizaron 18 pruebas cutáneas a cada uno, asignados por orden consecutivo entre junio de 2013 y julio de 2015. Como instrumentos de recogida de información se utilizaron la historia clínica, registro de pacientes atendidos y una encuesta confeccionada y validada en nuestra institución. Las variables de estudio fueron edad, sexo y sensibilización cutánea hacia Dermatophagoides pteronyssinus, Dermatophagoides farinae, Blomia tropicalis, Alternaria alternata, Aspergillus fumigatus, Cladosporium herbarum, Penicillium notatum, Acer negundo, Betula verrucosa, Cupressus arizonica, Eucalyptus globulus, Salix fragilis, Cynodon dactylon, Lolium perenne, Poa pratensis, Amaranthus retroflexus, Ambrosia trifida y Chenopodium album. La prueba de Prick se consideró positiva cuando los habones fueron ≥3 mm. Se realizaron 6300 pruebas cutáneas, la mayor prevalencia de sensibilización fue para los ácaros Dermatophagoides pteronyssinus (90% y Dermatophagoides farinae (66%, seguido del hongo Alternaria alternata (23% y pólenes Chenopodium álbum (20%, Amaranthus retroflexus (19% y Salix fragilis (21%. Se concluye que los ácaros Dermatophagoides pteronyssinus y Dermatophagoides farinae constituyeron la causa de mayor sensibilización cutánea en pacientes con rinitis alérgica.

  15. Mite-control activities of active constituents isolated from Pelargonium graveolens against house dust mites.

    Science.gov (United States)

    Jeon, Ju-Hyun; Kim, Hyung-Wook; Kim, Min-Gi; Lee, Hoi-Seon

    2008-10-01

    The mite-control activities of materials obtained from Pelargonium graveolens oil against Dermatophagoides farinae and D. pteronyssinus were examined using an impregnated fabric disk bioassay and were compared with those shown by commercial benzyl benzoate and N,N-diethylm- toluamide (DEET). Purification of the biologically active constituents from P. graveolens oil was done by silica gel chromatography and high performance liquid chromatography. The structures of the active components were analyzed by EI/MS, (1)H-NMR, (13)C-NMR, (1)H-(13)C COSYNMR, and DEPT-NMR spectra, and were identified as geraniol (C(10)H(18)O, MW 154.25, trans-3,7-dimethyl-2,6- octadien-1-ol) and beta-citronellol (C(10)H(20)O, MW 156.27, 3,7-dimethyl-6-octen-1-ol). Based on the LD50 values, the most toxic compound was geraniol (0.26 microg/cm(2)), followed by beta-citronellol (0.28 microg/cm(2)), benzyl benzoate (10.03 microg/ cm(2)), and DEET (37.12 microg/cm(2)) against D. farinae. In the case of D. pteronyssinus, geraniol (0.28 microg/cm(2)) was the most toxic, followed by beta-citronellol (0.29 microg/cm(2)), benzyl benzoate (9.58 microg/cm(2)), and DEET (18.23 microg/cm(2)). These results suggest that D. farinae and D. pteronyssinus may be controlled more effectively by the application of geraniol and beta-citronellol than benzyl benzoate and DEET. Furthermore, geraniol and beta-citronellol isolated from P. graveolens could be useful for managing populations of D. farinae and D. pteronyssinus.

  16. [Ecological study of Pyroglyphides mites in house dust in the Grenoble area. A qualitative approach and incidence of various parameters: seasons, altitude, temperature and relative dampness in their multiplication].

    Science.gov (United States)

    Lascaud, D

    1978-01-01

    This study concerns the analysis of 70 samples of dust mites taken periodically from 13 sample areas or mattresses at different altitudes. It shows the presence of Pyroglyphid mites in all the mattresses studied. Four species were found: Dermatophagoides pteronyssinus, Dermatophagoides farinae, Euroglyphus maynei and Dermatophagoides evansi. From a quantitative point of view, the statistical analysis of the results obtained allows the following conclusions to be drawn: -- All the Pyroglyphids (adults and young) undergo seasonal fluctuations at low, middle and high altitude. In the plains and at middle altitude these mites have a seasonal maximum and minimum. At high altitude only the seasonal maximum can be determined. -- The number of Pyroglyphids found is much higher in the plaine than at middle altitude and at middle altitude than at high altitude. The influence of altitude can be demonstrated with certitude. -- The conditions of temperature and relative dampness most favourable to the multiplication of mites are found in the plaine. At middle and high altitude these conditions are only met in summer. In flat country the statistical analysis allows us to show the existance of a correlation between the rate of dampness and the number of Pyroglyphids, and more specially, between the dampness rate and Dermatophagoides farinae, a predominant species at this sampling area. At middle and high altitude such a correlation cannot be shown clearly. At the three altitudes considered, no correlation can be established between the temperature and the number of Pyroglyphid mites found.

  17. Asthma, Airway Symptoms and Rhinitis in Office Workers in Malaysia: Associations with House Dust Mite (HDM) Allergy, Cat Allergy and Levels of House Dust Mite Allergens in Office Dust.

    Science.gov (United States)

    Lim, Fang Lee; Hashim, Zailina; Than, Leslie Thian Lung; Md Said, Salmiah; Hisham Hashim, Jamal; Norbäck, Dan

    2015-01-01

    A prevalence study was conducted among office workers in Malaysia (N= 695). The aim of this study was to examine associations between asthma, airway symptoms, rhinitis and house dust mites (HDM) and cat allergy and HDM levels in office dust. Medical data was collected by a questionnaire. Skin prick tests were performed for HDM allergens (Dermatophagoides pteronyssinus, Dermatophagoides farinae) and cat allergen Felis domesticus. Indoor temperature and relative air humidity (RH) were measured in the offices and vacuumed dust samples were analyzed for HDM allergens. The prevalence of D. pteronyssinus, D. farinae and cat allergy were 50.3%, 49.0% and 25.5% respectively. Totally 9.6% had doctor-diagnosed asthma, 15.5% had current wheeze and 53.0% had current rhinitis. The Der p 1 (from D. pteronyssinus) and Der f 1 (from D. farinae) allergens levels in dust were 556 ng/g and 658 ng/g respectively. Statistical analysis was conducted by multilevel logistic regression, adjusting for age, gender, current smoking, HDM or cat allergy, home dampness and recent indoor painting at home. Office workers with HDM allergy had more wheeze (p= 0.035), any airway symptoms (p= 0.032), doctor-diagnosed asthma (p= 0.005), current asthma (p= 0.007), current rhinitis (p= 0.021) and rhinoconjuctivitis (pallergy was associated with wheeze (p= 0.021), wheeze when not having a cold (p= 0.033), any airway symptoms (p= 0.034), doctor-diagnosed asthma (p= 0.010), current asthma (p= 0.020) and nasal allergy medication (p= 0.042). Der f 1 level in dust was associated with daytime breathlessness (p= 0.033) especially among those with HDM allergy. Der f 1 levels were correlated with indoor temperature (pallergies were common and independently associated with asthma, airway symptoms and rhinitis. Der f 1 allergen can be a risk factor for daytime breathlessness.

  18. [The relationship between the skin allergy test and house dust mites].

    Science.gov (United States)

    Atambay, Metin; Aycan, Ozlem M; Yoloğlu, Saim; Karaman, Ulkü; Daldal, Nilgün

    2006-01-01

    Since 1960 it has been known that house dust mites are related to allergy and that they cause pulmonary tract diseases. There are various house dust mites and among these Dermatophagoides pteronyssinus (Trouessart, 1897) and Dermatophagoides farinae (Hughes, 1961) are best known with regard to their medical importance and morphological characteristics. Skin tests are used to determine the role of mites in allergic diseases. The tests are performed by using D. pteronyssinus and D. farinae antigens. In order to study, the concordance between the skin test results and the presence of house dust mites, dust samples from the houses of 49 patients diagnosed with allergic diseases who underwent skin tests were taken to investigate the presence of mites in dust. House dust mites were determined in 23 (46.3%) of the houses. Mites were found in the houses of 15 (50.0%) of 30 patients with positive skin test results and 8 (42.0%) of 19 patients with negative skin test results. There was no significant difference between the skin test positivity and negativity in the presence of house dust mites (P>0,005). In conclusion, we thought that it was necessary to evaluate the presence of mites in the houses of people who have allergic symptoms even if they had negative skin test results.

  19. Correlations Between Allergen-Specific IgE Serum Levels in Patients With Ocular Allergy.

    Science.gov (United States)

    Polido, Júlia Gomes Fernandes; Cabral, Thiago; Perini, Paula de Resende Campos; Fernandes, Maria de Fátima Marcelos; de Freitas, Denise; dos Santos Araújo, Maria Emília Xavier; Serracarbassa, Pedro Durães

    2015-09-01

    To evaluate ocular allergies in patients at the Hospital do Servidor Publico Estadual de Sao Paulo (HSPE) and the correlations with serum allergen-specific immunoglobulin E levels. We performed a longitudinal study of patients with ocular allergies who were treated at the Cornea and Immunology and Allergy Department. Patients underwent an ophthalmologic examination to identify their primary presenting signs and symptoms. The allergy types were divided into 4 groups. We conducted the following laboratory tests and measurements: blood count, eosinophil count, total serum IgE, and specific IgE. Among 61 patients, 16 (26.2%) had a clinical diagnosis of seasonal allergic conjunctivitis, 23 (37.7%) had perennial allergic conjunctivitis, 19 (31.1%) had vernal keratoconjunctivitis, and 3 (4.9%) had atopic keratoconjunctivitis. Mixed dust mites were positive in 94.9% of patients. Dermatophagoides pteronyssinus (dp) and Dermatophagoides farinae (df) antigens were positive in 93.2% of patients followed by Blattella germanica, Blomia tropicalis, and mixed animal epithelia (81%, 75.9%, and 25.8%, respectively). Perennial allergic conjunctivitis was the most prevalent disorder and demonstrated higher positivity in class V/VI for specific antigens (mixed dust mites, dp, and df), indicating high antigenicity. Dust mites, D. pteronyssinus, D. farinae, B. germanica, and B. tropicalis were the primary triggers of the studied ocular allergies.

  20. 粉尘螨两种溶性抗原糖蛋白的组分与糖链研究%STUDY ON THE COMPONENTS AND GLUCOSIDE CHAINS OF GLUCOPROTEIN FROM TWO KINDS OF DERMATOPHAGOIDES FARINAE ANTIGENS

    Institute of Scientific and Technical Information of China (English)

    刘爱芹; 张唯哲; 中村·健; 蔺淑芳; 王光跃

    2004-01-01

    目的研究水溶性和尿素溶性粉尘螨糖蛋白的组分及结合糖链的构成. 方法用粉尘螨脱脂干粉提取水溶性抗原(Der fA)和尿素溶性抗原(Der fU),经SDS-PAGE,Western blot,免疫染色,胶体金染色和糖定性检测两种抗原糖蛋白的组分,并用9种HRP-植物血凝素进行结合糖链鉴定. 结果 Der fA显示4条蛋白带,分别为18 ku、30 ku、50 ku和58 ku,Der fU显示2条蛋白带(46 ku、58 ku),其中58 ku糖蛋白检测阳性.Der fA存在α-甘露糖(α-Man)、N-乙酰葡萄糖胺(GlcNAC)、α-葡萄糖(α-Glc),不存在寡糖(Oligosaccharide)、岩藻糖(α-Fuc)、β-半乳糖(β-Gal)、β-半乳糖-乙酰氨基半乳糖(β-Gal-GalNAC)及唾液酸(AS);Der fU只存在α-甘露糖,不存在其它7种糖链和唾液酸. 结论α-甘露糖是粉尘螨优势糖蛋白,其次是N-乙酰葡萄糖胺和α-葡萄糖.

  1. 粉尘螨Ⅲ类重组变应原对哮喘小鼠免疫治疗的效果%Effect of Immunotherapy with Recombinant Allergen Group 3 from Dermatophagoides farinae in Asthma Mice

    Institute of Scientific and Technical Information of China (English)

    李娜; 姜玉新; 刁吉东; 赵蓓蓓; 李朝品

    2014-01-01

    目的 探讨粉尘螨Ⅲ类重组变应原(rDer f3)对过敏性哮喘小鼠的免疫治疗效果. 方法 随机将40只BALB/c小鼠均分为4组,哮喘组、免疫治疗组、卵清蛋白(OVA)组和PBS组,分别在第0、第7和第14天哮喘组和免疫治疗组每鼠经腹腔注射100 μl致敏液(含rDerf 3 10 μg);卵清蛋白组每鼠经腹腔注射100 μl致敏液(含OVA 10 μg);PBS组则以PBS代替致敏液.第21天起,哮喘组和免疫治疗组小鼠用rDerf3进行滴鼻激发试验,连续7d,免疫治疗组小鼠在第25 ~27天滴鼻激发前30 min,用100 μg rDerf3纯化蛋白皮下注射进行特异性免疫治疗.PBS组和卵清蛋白组则分别用PBS和OVA进行滴鼻激发和腹腔注射,最后1次滴鼻激发24 h后脱臼处死小鼠.收集各组小鼠支气管肺泡灌洗液(BALF)进行白细胞和嗜酸粒细胞计数;对肺组织病理切片进行HE染色,镜下观察肺组织炎症细胞浸润情况.ELISA检测BALF和脾细胞培养上清(SSCS)中白细胞介素-5(IL-5)和y干扰素(IFN-y)及血清中变应原特异性IgE、IgG2a抗体水平. 结果 肺组织病理切片结果显示,免疫治疗组小鼠炎症反应明显减轻.小鼠BALF中白细胞总数在免疫治疗组、卵清蛋白组和哮喘组分别为(7.03±1.38)×108/ml、(22.11±3.70)×108/ml和(22.75±3.24)×108/ml,免疫治疗组低于卵清蛋白组和哮喘组(P<0.01),嗜酸粒细胞的变化趋势与白细胞类似.免疫治疗组、卵清蛋白组和哮喘组BALF中IL-5的水平分别为(108.20±11.02) pg/ml、(182.04±13.94) pg/ml和(19533±1533) pg/1ml,SSCS中IL-5的水平分别为(98.34±13.06) pg/ml、(208.26±10.63) pg/ml和(179.54±13.65) pg/ml,免疫治疗组均明显低于卵清蛋白组和哮喘组(P<0.01).而IFN-y含量则高于卵清蛋白组和哮喘组(P<0.01).IgE水平免疫治疗组、卵清蛋白组和哮喘组分别为(9.12±3.78) IU/ml、(26.87±4.30) IU/ml和(35.25±8.84) IU/ml,与卵清蛋白组和哮喘组相比,免疫治疗组明显降低(P<0.01),而IgG2a水平(38.52±6.33) μml则显著升高(P<0.01). 结论 粉尘螨Ⅲ类变应原可逆转哮喘小鼠的变态反应性气道及肺部炎症.

  2. Hypersensitivity to house dust mite and cockroach is the most common allergy in north of iran.

    Science.gov (United States)

    Ghaffari, Javad; Khademloo, Mohammad; Saffar, Mohammad Jafar; Rafiei, Alireza; Masiha, Farzad

    2010-12-01

    Asthma and allergic rhinitis are among the most common diseases in the world. The aim of this study was to detect, by skin prick test, aeroallergens in allergic patients in Sari, Mazandaran in north of Iran. This is a prospective study of skin prick test of aeroallergens in asthma, allergic rhinitis and their combination with clinical diagnosis. Three hundred and seventy five cases aged between 5 to 50 years, were referred to Tooba and Boo-Ali allergic centers of Mazandaran University of Medical Sciences between December 2006 and July 2009. The aeroallergens studied included house dust mites (Dermatophagoides farinae, Dermatophagoides pteronyssinus), cockroaches, feather, aspergillus, Alternaria, pigweed, nettle, oak and maple. Of the studied individuals, 175 cases were males (46.7%) and 200 were females (53.3%), of which 156 (n=41.5%) reacted to allergen extracts. In asthma, allergic rhinitis and their combination, the respective positive percentages were 26.6%, 22.9%, and 32.6% for Dermatophagoides farinae; 26.6%, 25.3%, and 23.3% for Dermatophagoides pteronyssinus; 12.7%, 17.4%, and 11.6% for cockroaches and 16.5%, 4.7%, and 7.0% for the feather. Other allergens were positive up to 5 percent. Total IgE levels were elevated in 56.4%, 53% and 60.5% of asthmatic, allergic rhinitis and the combination group, respectively. Eosinophils count was elevated in 40.5%, 33.2% and 37.2% of the same groups, respectively. The hypersensitivity to house dust mites is very common in north of Iran which may be attributed to the warm and humid weather of this area.

  3. Sensitization rates of airborne pollen and mold in children

    Directory of Open Access Journals (Sweden)

    So Hyun Park

    2012-09-01

    Full Text Available &lt;B&gt;Purpose:&lt;/b&gt; Aeroallergens are important causative factors of allergic diseases. Previous studies on aeroallergen sensitization rates investigated patients groups that had visited pediatric allergy clinics. In contrast, we investigated sensitization rates in a general population group of elementary school to teenage students in Incheon, Jeju, and Ulsan. &lt;b&gt;Methods:&lt;/B&gt; After obtaining parental consent, skin-prick tests were performed on 5,094 students between March and June 2010. Elementary school students were tested for 18 common aeroallergens, whereas middle and high school students were tested for 25 allergens. The 25 allergens included &lt;I&gt;Dermatophagoides pteronyssinus, Dermatophagoides farinae&lt;/I&gt;, pollen (birch, alder, oak, Japanese cedar, pine, willow, elm, maple, Bermuda grass, timothy grass, rye grass, orchard grass, meadow grass, vernal grass, mugwort, Japanese hop, fat hen, ragweed, and plantain, and mold (&lt;I&gt;Penicillatum, Aspergillus, Cladosporium, and Alternaria&lt;/I&gt;. &lt;b&gt;Results:&lt;/b&gt; The sensitization rates in descending order were 25.79% (&lt;I&gt;D. pteronyssinus&lt;/I&gt; , 18.66% (&lt;I&gt;D. farinae&lt;/I&gt; , 6.20% (mugwort, and 4.07% (willow in Incheon; 33.35% (&lt;I&gt;D. pteronyssinus&lt;/I&gt; , 24.78% (&lt;I&gt;D. farinae&lt;/I&gt;, 15.36% (Japanese cedar, and 7.33% (Alternaria in Jeju; and 32.79% (D. pteronyssinus, 30.27% (D. farinae, 10.13% (alder, and 8.68% (birch in Ulsan. The dust mite allergen showed the highest sensitization rate among the 3 regions. The sensitization rate of tree pollen was the highest in Ulsan, whereas that of Alternaria was the highest in Jeju. The ragweed sensitization rates were 0.99% in Incheon, 1.07% in Jeju, and 0.81% in Ulsan. &lt;b&gt;Conclusion:&lt;/b&gt; The

  4. Sensitization according to skin prick testings in atopic patients with asthma or rhinitis at 24 allergy clinics in Northern Europe and Asia

    Directory of Open Access Journals (Sweden)

    Nils E Eriksson

    1998-01-01

    Full Text Available Skin prick tests (SPT were performed on 2113 atopic patients (407 children and 1 706 adults with asthma and/or rhinitis at 24 allergy clinics in Iceland, Norway, Denmark, Sweden, Estonia, Lithuania and Russia. Test extracts were Dermatophagoides pteronyssinus (D. pteronyssinus, Dermatophagoides farinae [D. farinae, cat, dog, horse, birch, timothy, mugwort, Cladosporium, Alternaria, cockroach, chironomids (red mosquito larvae, RML and shrimp. Among the allergens, timothy followed by cat, birch and dog gave the highest number of positive SPT. Positive SPT with house dust mites (HDM, furred animals, RML and Cladosporium were more common in asthmatics than in patients with rhinitis; birch and timothy more common in patients with rhinitis. Sensitization against D. pteronyssinus, horse, timothy and Cladosporium was more common in men than in women. Although the general sensitization pattern of the atopic patients at the participating centers showed similarities, there were also significant differences between centers. Positive SPT with furred animals were most prevalent in Northern and Central Sweden and St Petersburg, and least common in Siberia and Denmark. Pollen allergy was most common in Novosibirsk and on the west coast of Sweden, and less common in Vladivostok. Sensitization against HDM was most common in Lithuania and least prevalent in Northern Sweden and Finland. Insect allergens gave the most positive reactions in St Petersburg and the least positive reaction in Novosibirsk. Sensitization against multiple allergens was found in 74% of the patients and a mono-allergy in 26%. The degree of atopy was higher in males than in females and higher in asthmatics than in patients with rhinitis. The month of birth of the patients did not influence significantly the test results. It is concluded that although the sensitization pattern shows similarities in different regions, it is also influenced to some extent by residence as well as by diagnosis, sex

  5. RAST studies : IgE antibodies to Dermatogoides pteronyssinus (house dust mite), Aspergillus fumigatus and beta-lactoglobulin in sudden death in infancy syndrome (SDIS).

    Science.gov (United States)

    Turner, K J; Baldo, B A; Hilton, J M

    1975-01-01

    The incidence of 2.5 SDIS cases per 1,000 live births found in Western Australia is in agreement with figures reported for other centres. While the age range of SDIS victims extended from two weeks to 15 months, 57 per cent of deaths occurred in children of two to four months of age. Boys outnumbered girls 1.6:1. Environmental factors are implicated in that the majority of deaths occurred in a biphasic distribution - autumn and late winter months. No significant differences were observed in total IgE levels in serum from SDIS victims, post mortem children who died in trauma of known aetiology and live control children of the same age range. Serum IgE antibodies to D.pteronyssinus were found in 37% of SDIS victims compared with 7% of matched controls (post mortem plus live groups). IgE antibodies to beta-lactoglobulin, the major allergen of cow's milk, appeared with twice the frequency in SDIS vs. control group but both groups showed a similar incidence of antibodies to the allergens of Aspergillus fumigatus. The prevalence of IgE antibodies to D.pteronyssinus in SDIS victims who died in the late winter -- early spring period was double that found in the group who died in the autumn period. Sixtyfour percent of the SDIS victims had antibodies to two or more of the three allergens tested while the control sera were positive to only one allergen. These results support the hypothesis that anaphylaxis induced by immediate hypersensitivity to D.pteronyssinus in particular may be one of the causative factors in SDIS in Western Australia.

  6. Influence of degree of specific allergic sensitivity on severity of rhinitis and asthma in Chinese allergic patients

    Directory of Open Access Journals (Sweden)

    Zhao Changqing

    2011-07-01

    Full Text Available Abstract Background The association between sensitizations and severity of allergic diseases is controversial. Objective This study was to investigate the association between severity of asthma and rhinitis and degree of specific allergic sensitization in allergic patients in China. Method A cross-sectional survey was performed in 6304 patients with asthma and/or rhinitis from 4 regions of China. Patients completed a standardized questionnaire documenting their respiratory and allergic symptoms, their impact on sleep, daily activities, school and work. They also underwent skin prick tests with 13 common aeroallergens. Among the recruited subjects, 2268 provided blood samples for serum measurement of specific IgE (sIgE against 16 common aeroallergens. Results Significantly higher percentage of patients with moderate-severe intermittent rhinitis were sensitized to outdoor allergens while percentage of patients sensitized to indoor allergens was increased with increasing severity of asthma. Moderate-severe intermittent rhinitis was associated with the skin wheal size and the level of sIgE to Artemisia vulgaris and Ambrosia artemisifolia (p Dermatophagoides (D. pteronyssinus and D. farinae (p Conclusions Artemisia vulgaris and Ambrosia artemisifolia sensitizations are associated with the severity of intermittent rhinitis and D. pteronyssinus and D. farinae sensitizations are associated with increasing severity of asthma in China. Increase in number of allergens the patients are sensitized to may also increase the severity of rhinitis and asthma.

  7. Diversity of House Dust Mite Species in Xishuangbanna Dai, a Tropical Rainforest Region in Southwest China

    Directory of Open Access Journals (Sweden)

    Jing-Miao Yu

    2015-01-01

    Full Text Available Purpose. To survey the species diversity of home dust mites (HDM in Xishuangbanna, a tropical rainforest region in Southwest China. Methods. From August 2010 to January 2011, mite-allergic patients and healthy controls were invited to participate. Dust samples from the patients’ homes were collected, and mites in the samples were isolated. Permanent slides were prepared for morphologically based species determination. Results. In total, 6316 mite specimens of morphologically identifiable species were found in 233 dust samples taken from 41 homes. The result shows that the mite family of Pyroglyphidae occupied the highest percentage of the total amount of mites collected, followed by Cheyletidae family. The most common adult Pyroglyphidae mites were Dermatophagoides (D. farinae, D. pteronyssinus, and D. siboney. The most common mites found from other families were Blomia tropicalis, Tyrophagus putrescentiae, and Aleuroglyphus ovatus. Four main allergenic dust mite species D. farinae, D. pteronyssinus, D. siboney, and Blomia tropicalis were found to be coinhabiting in 6/41 homes. Conclusion. The HDM population in homes in Xishuangbanna, a tropical rainforest region in Southwest China, has its own characteristics. It has rich dust mite species and the dust mite densities do not show significant variation across seasons.

  8. Qualitative and quantitative assessment of mites (Acari in domiciliary dust in rural dwellings in the "Zona da Mata" region, Minas Gerais, Brazil

    Directory of Open Access Journals (Sweden)

    Oliveira Aristeu José de

    2003-01-01

    Full Text Available From July to September, 2000 (winter, and from January to March, 2001 (summer, 30 dust samples were collected for each season, from beds of rural dwellings located in farms in the geographical area named "Zona da Mata", Minas Gerais, Brazil. After being sorted, the mites were identified and quantified. The prevalence of mites in the samples was 100%. 891 mites were found in winter (22.97%, and 2988 in summer (77.03%. In winter, Dermatophagoides pteronyssinus (Trouessart, 1897 was the most prevalent (55.00%, followed by Blomia tropicalis (Bronswijk, Cock & Oshima, 1973 (27.06%, Euroglyphus maynei (Cooreman, 1950 (8.85%, and predator mites from Cheyletidae family (8.07%. In summer, the most prevalent species was B. tropicalis (47.79%, followed by D. pteronyssinus (43.38%, Cheyletidae (6.87%, and E. maynei (1.28%. Few Dermatophagoides farinae (Hughes, 1961, Chortoglyphus arcuatus (Troupeau, 1879, and mites from Tarsonemidae and Cunaxidae families were found, the last two occurring only in summer. No mites from Acaridae family were found. The greatest number of immature forms found in summer suggested a greater breeding activity in this season. It was also noted that different building materials and varied cleaning routines may influence the population size of domiciliary dust mites.

  9. Indoor Allergen Levels and Household Distributions in Nine Cities Across China

    Institute of Scientific and Technical Information of China (English)

    ZHENG Yi Wu; NORBACK Dan; GJESING Birgitte; ZHONG Nan Shan; SPANGFORT DMichael; LAI Xu Xin; ZHAO De Yu; ZHANG Chun Qing; CHEN Jian Jun; ZHANG Luo; WEI Qing Yu; CHEN Shi; LIU En Mei

    2015-01-01

    Objective Chinese allergic subjects have high levels of sensitization to house dust mite (HDM) and other indoor allergens. This study quantifies common indoor allergen levels in Chinese households. Methods Dust samples were collected from nine cities. Major allergens Der p 1 and Der f 1 from Dermatophagoides pteronyssinus and D. farinae, and specific antigens of Blomia tropicalis, Tyrophagus putrescentiae, Acarus siro, and cockroach species Blattella germanica and Periplaneta americana were measured by ELISA. Results HDM allergens were found in dust samples from bedding in 95% of the Chinese households. The median levels varied from Conclusion HDM allergens are present in bedding dust samples from most Chinese households. Cities in southern and central China have relatively high levels of HDM major allergens compared to cities in northern and western China. Antigens of storage mites and cockroaches are not as common as HDM allergens.

  10. House dust mites: a risk factor to be considered for occupational safety or source of work-related allergens.

    Science.gov (United States)

    Saleh, Ahmed Megahed Ahmed; Ali, Hisham Abd El-Raouf; Ahmed, Salwa Abdalla Mohamed; Mohammad, Naema Mahmoud; Morsy, Tosson A

    2013-12-01

    House dust mites (HDM) can be found worldwide where human beings live independent from the climate and are a major source of multiple allergens. Mite allergens sensitize and induce perennial rhinitis, asthma, or atopic dermatitis in a large portion of patients with allergic disease particularly children. There is convincing evidence that avoidance of mite allergen can effectively reduce allergic symptoms. This study examined dust from a military hospital and the private home of some nursing staff. A total of seven species of mites belonging to six genera were recovered. The commonest species was Dermatophagoides farinae followed by D. pteronyssinus and the lowest Laelaps nuttalli. Besides, the 7th mite or Parasitus consanguineous live free on dust as a bio-control agent of mites. The presence of mites in and out doors in a hospital and dwellings of medical personnel pave the way to consider HDM as occupational or nosocomial Allergens.

  11. Prolonged effect of allergen sublingual immunotherapy for house dust mites in elderly patients.

    Science.gov (United States)

    Bozek, Andrzej; Starczewska-Dymek, Liwia; Jarzab, Jerzy

    2017-07-01

    The prolonged effect of allergen immunotherapy is unknown, especially in older patients. To analyze the 3-year effect of sublingual allergen-specific immunotherapy (SLIT) to house dust mites in elderly patients with allergic rhinitis. Forty-seven elderly patients (65.78 ± 4.89 years old) underwent SLIT to house dust mites and were monitored for 3 years and compared with a placebo group. SLIT was performed with the use of oral Staloral 300 SR Der p and Der f 50/50% extract (Stallergens Greer, London, United Kingdom) or placebo. Symptoms and medication score, represented by the average adjusted symptom score (AAdSS), serum level of immunoglobulin (Ig) G4 to Dermatophagoides pteronyssinus, Dermatophagoides farinae, Der p 1, and Der p 2, and quality of life, were assessed immediately after SLIT and 3 years later. The AAdSS was significantly decreased after SLIT, and the level remained low during the 3 years after SLIT compared with placebo. Serum-specific IgG4 against D pteronyssinus, D farinae, Der p 1, and Der p 2 increased during the SLIT trial in the study group. For the 3 years of observation after SLIT, there were no significant changes of specific IgG4 levels against the analyzed allergens compared with results just after SLIT. Quality of life based on the Rhinoconjunctivitis Quality of Life Questionnaire score was significantly decreased in patients who received SLIT, from 1.48 (95% confidence interval 1.33-1.79) to 0.98 (95% confidence interval 0.67-1.07; P mites was observed in elderly patients with allergic rhinitis. Further trials are needed to confirm this effect. ClinicalTrials.gov Identifier: NCT01605760. Copyright © 2017 American College of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.

  12. [Sensitization to house dust and storage mites in allergic adults from the South of Mexico city].

    Science.gov (United States)

    Ramírez-Heredia, Jennifer; Oífarrill-Romanillos, Patricia María; Guidos-Fogelbach, Guillermo; Miyagui-Nakamura, Roberto Ken; Segura-Méndez, Nora Hilda

    2013-01-01

    Mites are the most common cause of respiratory allergy. Sensitization to house dust mites is estimated at 30%. Families Euroglyphus and Dermatophagoides, are the most important. Recently, storage mites, from the families Acaridae and Glyciphagidae, have become more important as a cause of allergic respiratory diseases. The aim of this study was to identify sensitization to house dust and storage mites by skin tests in adult patients with allergic respiratory diseases. This is a descriptive study in patients with asthma and/or allergic rhinitis, with the approval of the local research committee 2009-3601-46. Patients underwent skin prick tests, with seven standardized extracts of mites (IPI ASACR). The results were analyzed with descriptive statistics. A total of 150 patients, 109 women and 41 men, with an average age 31 years (±11) were studied. The more common allergic diseases in this group of patients were: asthma and rhinitis 72 and 26%, respectively, and asthma plus rhinitis 3.2%. Positive skin tests for Dermatophagoides pteronyssinus and Dermatophagoides farinae were 82.1% 71.5%, respectively, and for storage mites: A. siro 51.7%, Tyrophagus 47.4%, Glycyphagus 47.7%, B. tropicalis 39.7% and Lepidoglyphus 39.1%. Storage mites produced greater sensitization than house dust mites, 32% vs 10%. The results show that adult patients with respiratory allergy, in southern Mexico City, have a higher rate of sensitization to storage mites than the one reported in the literature.

  13. Significance of specific IgG against sensitizing antigens in extrinsic allergic alveolitis: serological methods in EAA.

    Science.gov (United States)

    Sterclova, M; Vasakova, M; Metlicka, M

    2011-01-01

    The aim of our study is to find differences in IgG in sera of potentially exposed and nonexposed individuals and to detect differences in concentrations of specific serum IgG among subjects with and without EAA. Seventy-two patients being followed for suspected interstitial lung disease were included. Specific IgG in sera were established by ImmunoCAP. Serum concentrations of Aspergillus fumigatus, Candida albicans IgG and mixture of moulds IgG were higher in subjects with exposure to relevant inhalation antigens (p<0.05). Patients exposed to parrot and mammal hair mixture had higher serum concentration of specific IgG (p<0.05). Subjects without exposure to mites had lower serum IgG to Dermatophagoides pteronyssinus, Dermatophagoides farinae, Dermatophagoides microceras and Glycophagus domesticus (p<0.05). Higher concentration of serum specific IgG may show previous exposure to this antigen. Even though mite specific IgG are not commonly tested in EAA patients, we suggest their immunomodulatory activity may influence susceptibility to other inhalation antigens. Copyright © 2010. Published by Elsevier España.

  14. Evaluation of skin sensitivity in dogs bearing allergic dermatitis to standardized allergenic extract of house dust and storage mites Avaliação da sensibilidade de cães com dermatite alérgica a extratos padronizados de ácaros da poeira domiciliar

    Directory of Open Access Journals (Sweden)

    Victor E.S. Cunha

    2007-08-01

    Full Text Available The objective of the study was to evaluate whether allergenic extracts of five house dust and storage mite species standardized for humans might be used for the diagnosis of canine atopic dermatitis (CAD. Extracts of Dermatophagoides pteronyssinus (Pyroglyphidae, D. farinae (Pyroglyphidae, Blomia tropicalis (Glycyphagidae, Lepidoglyphus destructor (Glycyphagidae and Tyrophagus putrescentiae (Acaridae were evaluated by intradermal testing in 20 healthy dogs (control and 25 dogs with allergic dermatitis. A significant difference in the response was observed between the two groups (pO presente trabalho teve como objetivo avaliar se extratos alergênicos de cinco espécies de ácaros da poeira domiciliar e produtos armazenados, padronizados para humanos, podem ser utilizados no diagnóstico da dermatite atópica canina. Extratos de Dermatophagoides pteronyssinus (Pyroglyphidae, D. farinae (Pyroglyphidae, Blomia tropicalis (Glycyphagidae, Lepidoglyphus destructor (Glycyphagidae e Tyrophagus putrescentiae (Acaridae foram avaliados através de testes intradérmicos em 45 cães, dos quais 20 normais e 25 com dermatite alérgica. Uma diferença significativa foi observada no padrão de respostas obtidas dos dois grupos (p<0.05. Apenas um animal (5% do grupo controle reagiu ao teste cutâneo, enquanto que no grupo dos alérgicos 14 cães (56% apresentaram pelo menos uma reação positiva (odds ratio = 24.2. As maiores freqüências de reações positivas observadas no grupo dos alérgicos foram aos extratos de T. putres-centiae ou L. destructor, cada um induzindo reações em 10(40% cães. Os extratos de D. farinae, D. pteronyssinus e B. tropicalis foram responsáveis por reações positivas em 7(28%, 3(12% e 3(12% cães, respectivamente. Os extratos padronizados para humanos avaliados no presente estudo podem ser utilizados como complemento no diagnóstico da doença, assim como na seleção de alérgenos para a imunoterapia alérgeno-específica.

  15. Asthma, Airway Symptoms and Rhinitis in Office Workers in Malaysia: Associations with House Dust Mite (HDM Allergy, Cat Allergy and Levels of House Dust Mite Allergens in Office Dust.

    Directory of Open Access Journals (Sweden)

    Fang Lee Lim

    Full Text Available A prevalence study was conducted among office workers in Malaysia (N= 695. The aim of this study was to examine associations between asthma, airway symptoms, rhinitis and house dust mites (HDM and cat allergy and HDM levels in office dust. Medical data was collected by a questionnaire. Skin prick tests were performed for HDM allergens (Dermatophagoides pteronyssinus, Dermatophagoides farinae and cat allergen Felis domesticus. Indoor temperature and relative air humidity (RH were measured in the offices and vacuumed dust samples were analyzed for HDM allergens. The prevalence of D. pteronyssinus, D. farinae and cat allergy were 50.3%, 49.0% and 25.5% respectively. Totally 9.6% had doctor-diagnosed asthma, 15.5% had current wheeze and 53.0% had current rhinitis. The Der p 1 (from D. pteronyssinus and Der f 1 (from D. farinae allergens levels in dust were 556 ng/g and 658 ng/g respectively. Statistical analysis was conducted by multilevel logistic regression, adjusting for age, gender, current smoking, HDM or cat allergy, home dampness and recent indoor painting at home. Office workers with HDM allergy had more wheeze (p= 0.035, any airway symptoms (p= 0.032, doctor-diagnosed asthma (p= 0.005, current asthma (p= 0.007, current rhinitis (p= 0.021 and rhinoconjuctivitis (p< 0.001. Cat allergy was associated with wheeze (p= 0.021, wheeze when not having a cold (p= 0.033, any airway symptoms (p= 0.034, doctor-diagnosed asthma (p= 0.010, current asthma (p= 0.020 and nasal allergy medication (p= 0.042. Der f 1 level in dust was associated with daytime breathlessness (p= 0.033 especially among those with HDM allergy. Der f 1 levels were correlated with indoor temperature (p< 0.001 and inversely correlated with RH (p< 0.001. In conclusion, HDM and cat allergies were common and independently associated with asthma, airway symptoms and rhinitis. Der f 1 allergen can be a risk factor for daytime breathlessness.

  16. [Frequency of sensitization to mites, cockroach and shrimp in adults with respiratory allergy].

    Science.gov (United States)

    López-Rocha, Eunice; Rodríguez-Mireles, Karen; Gaspar-López, Arturo; Del Rivero-Hernández, Leonel; Segura-Méndez, Nora

    2014-01-01

    Sensitization to mites and cockroach are a common cause of respiratory allergy, especially in children. There is little information about its cross-reactivity with shrimp, which is common in adult patients. To determine the frequency of sensitization to house dust mites (Dermatophagoides pteronyssinus, Dermatophagoides farinae) and cockroach species Periplaneta americana, in adult patients attending to a third level hospital and to determine the frequency of sensitization to shrimp in patients with positive skin test to house dust mite and/or cockroach, in patients with clinical manifestations suggestive of allergy to shrimp. A retrospective study was done in which results were reviewed of 672 patients clinical records, attending to the Allergy and Clinical Immunology department in a third level hospital, those patients with positive skin test results to Dermatophagoides and/or Periplaneta americana were investigated for sensitization to shrimp through Prick-to-Prick test. Data from 672 patients were obtained, 69.8% (469) males, with a mean age of 33.5 years. Dermatophagoides positivity was 72.2% (486/672), and its relationship with other invertebrates that also have tropomyosin was 5.6% (4.9% with cockroach and 0.74% with shrimp). In our population, house dust mite sensitization was similar to that reported in the international literature. There was low positivity to cockroach, probably due to geographical conditions. There is very little information about shrimp's cross-reactivity with Dermatophagoides, despite its clinical significance, which requires more specific studies such as measurement of specific IgE to tropomyosin.

  17. Prevalence of allergen sensitization, most important allergens and factors associated with atopy in children

    Directory of Open Access Journals (Sweden)

    Raquel Prudente de Carvalho Baldacara

    Full Text Available CONTEXT AND OBJECTIVE: Knowledge of the profile of allergen sensitization among children is important for planning preventive measures. The objective of this study was to assess the prevalence and profile of sensitization to inhaled allergens and food among children and adolescents in an outpatient population in the city of Palmas. DESIGN AND SETTING: Cross-sectional study at outpatient clinics in Palmas, Tocantins, Brazil. METHODS: Ninety-four patients aged 1-15 years who were attending two pediatric outpatient clinics were selected between September and November 2008. All of the subjects underwent clinical interviews and skin prick tests. RESULTS: A positive skin prick test was observed in 76.6% of the participants (72.3% for inhalants and 28.9% for food allergens. The most frequent allergens were Dermatophagoides pteronyssinus (34%, cat epithelium (28.7%, dog epithelium (21.3%, Dermatophagoides farinae (19.1%, Blomia tropicalis (18.1%, cow's milk (9.6% and grasses (9.6%. A positive skin prick test correlated with a history of atopic disease (odds ratio, OR = 5.833; P = 0.002, a family history of atopic disease (OR = 8.400; P < 0.001, maternal asthma (OR = 8.077; P = 0.048, pet exposure (OR = 3.600; P = 0.012 and cesarean delivery (OR = 3.367; P = 0.019. CONCLUSION: Dermatophagoides pteronyssinus was the most frequent aeroallergen and cow’s milk was the most prevalent food allergen. There was a positive correlation between a positive skin prick test and several factors, such as a family history of atopic disease, maternal asthma, pet exposure and cesarean delivery.

  18. Comparative mites and cockroaches sensitization study in three cities of Mexico.

    Science.gov (United States)

    Cavazos Galván, Mario; Guerrero Núñez, Belinda; Ramírez Aragón, Daniel

    2008-01-01

    In Mexico there are few research papers dealing with mites and cockroaches sensibilizing in patients suffering from respiratory allergies and even less susceptibility comparative studies to the same between cities having different climatic and geographic characteristics. To research in a prospective study skin sensibilizing to eighy different mites and two cockroaches species in patients with respiratory allergies in three cities of Mexico: Monterrey, NL, Tampico, Tamps., and Irapuato, Gto., in order to determine if there are sensibilizing differences due to climatic and geographic conditions. Skin tests were performed by scarification in 58 patients suffering diverse respiratory allergies such as bronchial asthma, allergic rhinitis, allergic rhinoconjunctivitis and combinations of the same. Tests were performed on 18 patients from Monterrey, 20 from Tampico and 20 from Irapuato. Mites used were: Dermatophagoides pteronyssinus, Dermatophagoides farinae, Blomia tropicalis, Tyrophagus putrescientiae, Lepidoglyphus destructor, Euroglyphus maynei, Acarus siro, Dermatophagoides microceras and 2 cockroaches species, Periplaneta americana and Blatella germanica. Of the total 58 patients, the most important and frequent reactions were produced by D. pteronyssinus, D. farinae and E. maynei, which were found in 40, 33 and 32 patients respectively, then D. microceras in 21 patients, A. siro in 18, and B. tropicalis in 13 patients. In Tampico, the mite with the most frequent susceptibility was E. maynei, in Monterrey and Irapuato the most relevant were D. farinae and D. pteronyssinus, respectively. In Tampico, B. tropicalis was positive in 8 patients vs only 4 in Monterrey and 1 in Irapuato. The skin response to cockroaches was not important and the city with most positives was Irapuato with 6 patients responding to B. germanica, and 3 to P. americana; in Tampico 5 patients reacted to P. americana and 3 to B. germanica, and in Monterrey skin reactivity was slight and only 2

  19. Alleviation of Dermatophagoides microceras-induced allergy by an immunomodulatory protein, FIP-fve, from Flammulina velutipes in mice.

    Science.gov (United States)

    Chang, Yu-Chi; Hsiao, Yi-Min; Hung, Shao-Chi; Chen, Ya-Wen; Ou, Chu-Chyn; Chang, Wei-Ting; Lue, Ko-Huang; Ko, Jiunn-Liang

    2015-01-01

    Asthma is a major public health concern. Its greatest risk factor is house dust mite (HDM). Dermatophagoides microceras (Der m) is a type of HDM, and in central Taiwan, there is approximately 80% prevalence of sensitization to Der m. FIP-fve is a fungal immunomodulatory protein (FIP) isolated from the fungus Flammulina velutipes, and exhibits anti-inflammatory properties. To investigate whether FIP-fve affects Der m-induced asthma and inflammation, we evaluated hyper-responsiveness (AHR), pathological changes, and cytokines in mice. We demonstrated that oral FIP-fve decreased Der m-induced airway AHR, airway inflammation, cell infiltration, and expression of cytokines in the bronchoalveolar lavage fluid of Balb/c mice. The results of this study suggest that FIP-fve suppresses asthma, inflammation, and respiratory pathogenesis stimulated by Der m. FIP-fve is able to maintain immunomodulatory activity even in simulated gastric fluid and intestinal fluid. FIP-fve could be a safe and stable agent for suppression of allergic asthma.

  20. 成都地区859例慢性荨麻疹患者过敏原检测结果分析%Detection and analysis of allergens in 859 patients with chronic urticaria in Chengdu

    Institute of Scientific and Technical Information of China (English)

    宋黎; 叶俊儒; 陆茂; 唐懿; 刘彦麟

    2016-01-01

    Objective To analyze the allergens and sex ,age distribution of chronic urticaria in Chengdu .Methods Totally 859 patients with chronic urticaria were tested with 13 kinds inhaled allergens and 15 kinds of food allergens by skin prick test .Re‐sults The top 5 allergens were:dermatophagoides farinae、dermatophagoides pteronyssinus ,cockroach ,shrimp and sea‐crab .The positive rate of dermatophagoides farinae was 58 .7% ,dermatophagoides pteronyssinus which was 55 .1% took second place .No difference was found between sex ,more inhaled allergens were found positive than food allergens in both sex groups .The positive rate was higher in people younger than 60 .Conclusion Dermatophagoides farinae ,dermatophagoides pteronyssinus ,cockroach , shrimp and sea‐crab are the commonest allergens in Chengdu .The skin prick test is important in the individualized treatment of chronic urticaria and health education .It may also be helpful in the management of chronic allergic skin diseases .%目的:分析成都地区慢性荨麻疹的常见过敏原及其性别、年龄分布。方法采用变应原点刺液(商品名:阿罗格)对859例慢性荨麻疹患者实施13种吸入物及15种食物过敏原皮肤点刺试验。结果皮试阳性率最高的前5位变应原依次是:粉尘螨、屋尘螨、蟑螂、小虾和蟹肉。其中粉尘螨阳性率最高,为58.7%,屋尘螨次之,为55.1%。不同性别变应原阳性率差异无统计学意义,男女两组吸入组变应原阳性率明显高于食物组。儿童、青中年人群变应原阳性率更高。结论粉尘螨、屋尘螨、蟑螂、小虾和蟹肉等是成都地区慢性荨麻疹的主要过敏原。皮肤点刺试验可以明确过敏原,对于慢性荨麻疹的个体化治疗及健康教育起重要作用,并为该地区慢性病管理提供合理数据。

  1. Monthly variation of Dermatophagoides allergens and its influence on respiratory allergy in a high altitude environment (Quito, 2800 m a.s.l. in Andean Ecuador).

    Science.gov (United States)

    Valdivieso, R; Iraloa, V

    2011-01-01

    Seasonal variation of Dermatophagoides allergens and its influence in allergic respiratory airway diseases has not been investigated in Andean cities. The objective of this study was to evaluate those parameters in a city located in the Andean mountains. Der p1 and Der f1 were measured in dust samples from mattresses in 13 houses in Quito (2800 m above sea level). Samples were collected monthly from August 2004 to July 2005. Patients presenting to a local outpatient allergy clinic with asthma and rhinitis and isolated allergy to Dermatophagoides were analysed to determine if a correlation existed between seasonal Der allergen levels and the number of patients presenting with allergies. High levels of dust mites and humidity were observed throughout the year. The highest geometrical mean values of allergens were detected in April (Der p1, 10.15 μg/g) and May (Der f1, 13.03 μg/g), whilst the lowest levels were detected in August (Der p1, 4.26 μg/g), and September (Der f1, 1.4 μg/g). Of the 361 patients examined, 182 were allergic to Dermatophagoides, (45.6% asthmatics, 97.8% rhinitics, and 43.4% with both diseases). Patient presentation spiked in August, and from February to May. However, there was not a significant correlation between mite allergen concentrations and humidity or the number of patients presenting with allergies. Dust samples from mattresses in Quito revealed high concentrations of Der p1 and Der f1. We observed a trend towards increased presentation of asthmatic and rhinitic patients in the months with highest levels of allergens. Copyright © 2009 SEICAP. Published by Elsevier Espana. All rights reserved.

  2. Identificación de ácaros del polvo casero en colchones y almohadas de niños alérgicos de Santa Marta, Colombia

    Directory of Open Access Journals (Sweden)

    Jainy Meza Navarro

    2013-10-01

    Full Text Available ResumenLa prevalencia elevada de alergias en Colombia hace necesario investigar los factores que influye sobre su etiología. El objetivo de este estudio fue identificar la fauna de ácaros presente en los hogares de niños alérgicos de Santa Marta. Un total de 70 muestras de polvo fueron colectadas desde 42 casas por medio de la aspiración de colchones y almohadas, durante los meses de Julio a Octubre de 2007. Los ácaros fueron identificados bajo la luz de un microscopio usando claves taxonómicas, contados y expresados como ácaros por gramo de polvo. Los ácaros de la familia Pyroglyphidae fueron los más predominantes (84,58%, teniendo a Dermatophagoides farinae (23,85% como la especie más abundante, seguido por Dermatophagoides pteronyssinus (7,31%. Otros ácaros prevalentes fueron Cheyletus spp, Euroglyphus maynei y Blomia tropicales, con niveles superiores a los 500 ácaros/ gramo, considerado como de alto riesgo para sensibilización alérgica. Estos resultados contribuyen al conocimiento de la fauna de ácaros del polvo casero de Santa Marta, importante para el diagnóstico y terapia de las alergias. (Duazary 2008; 1: 24 - 31.AbstractThe increasing prevalence of allergic diseases in Colombia makes necessary to research about factors that influence their etiology. The aim of this study was to investigate the mite fauna present in homes of allergic child from Santa Marta. A total of 70 samples were collected from 42 houses through vacuuming mattresses and pillows, during the months of July to October 2007. Mites were identified under light microscopy using taxonomic keys, count and expressed as mites per gram of dust. The prevalent mite family was Pyroglyphidae (84.58%, having Dermatophagoides farinae as the major specie (23.85%, followed by D. pteronyssinus. Other mites prevalent were Cheyletus spp, Euroglyphus maynei and Blomia tropicalis, with levels up to 500 mites/ gram, considered like of high risk for the allergic sensitization

  3. Crystal Structures of Mite Allergens Der f 1 and Der p 1 Reveal Differences in Surface-Exposed Residues that May Influence Antibody Binding

    Energy Technology Data Exchange (ETDEWEB)

    Chruszcz, Maksymilian; Chapman, Martin D.; Vailes, Lisa D.; Stura, Enrico A.; Saint-Remy, Jean-Marie; Minor, Wladek; Pomés, Anna; (INDOOR Bio.); (CEAS); (Leuven); (UV)

    2009-12-01

    The Group 1 mite allergens, Der f 1 and Der p 1, are potent allergens excreted by Dermatophagoides farinae and Dermatophagoides pteronyssinus, respectively. The human IgE antibody responses to the Group 1 allergens show more cross-reactivity than the murine IgG antibody responses which are largely species-specific. Here, we report the crystal structure of the mature form of Der f 1, which was isolated from its natural source, and a new, high-resolution structure of mature recombinant Der p 1. Unlike Der p 1, Der f 1 is monomeric both in the crystalline state and in solution. Moreover, no metal binding is observed in the structure of Der f 1, despite the fact that all amino acids involved in Ca{sup 2+} binding in Der p 1 are completely conserved in Der f 1. Although Der p 1 and Der f 1 share extensive sequence identity, comparison of the crystal structures of both allergens revealed structural features which could explain the differences in murine and human IgE antibody responses to these allergens. There are structural differences between Der f 1 and Der p 1 which are unevenly distributed on the allergens' surfaces. This uneven spatial arrangement of conserved versus altered residues could explain both the specificity and cross-reactivity of antibodies against Der f 1 and Der p 1.

  4. Position paper prepared by the Section of Immunotherapy, Polish Society of Allergy

    Directory of Open Access Journals (Sweden)

    Marek Jutel

    2015-12-01

    Full Text Available SLIT ([i]sublingual immunotherapy[/i], induces allergen-specific immune tolerance by sublingual administration of a gradually increasing dose of an allergen. The mechanism of SLIT is comparable to those during SCIT (subcutaneous immunotherapy, with the exception of local oral dendritic cells, pre-programmed to elicit tolerance. In the SLIT dose, to achieve the same efficacy as in SCIT, it should be 50–100 times higher with better safety profile. The highest quality evidence supporting the efficacy of SLIT lasting 1 – 3 years has been provided by the large scale double-blind, placebo-controlled (DBPC trials for grass pollen extracts, both in children and adults with allergic rhinitis. Current indications for SLIT are allergic rhinitis (and conjunctivitis in both children and adults sensitized to pollen allergens (trees, grass, [i]Parietaria[/i], house dust mites ([i]Dermatophagoides pteronyssinus, Dermatophagoides farinae[/i], cat fur, as well as mild to moderate controlled atopic asthma in children sensitized to house dust mites. There are positive findings for both asthma and new sensitization prevention. Severe adverse events, including anaphylaxis, are very rare, and no fatalities have been reported. Local adverse reactions develop in up to 70 – 80% of patients. Risk factors for SLIT adverse events have not been clearly identified. Risk factors of non-adherence to treatment might be dependent on the patient, disease treatment, physician-patient relationship, and variables in the health care system organization.

  5. Sensitization Profile to Allergens in Patients Using Multi-Test II

    Science.gov (United States)

    Maniglia, Sergio Fabricio; Tsuru, Fernanda Miyoko; Santos, Victor Carvalho dos; Ueda, Denis Massatsugu

    2014-01-01

    Introduction Medical intervention in allergies has broadened its perspective, also focusing in the quality of life of patients. Patients are instructed, before using pharmacotherapy agents, to avoid the causal agent. Objective This study aims to analyze the sensitization profile of patients with allergic complaints and identify possible characteristics specific to each age group and gender. Methods A descriptive cross-sectional study included data collected from medical records (from Multi-Test II database, Lincoln Diagnostics Inc. Decatur, Illinois) of 1,912 patients who underwent skin prick test from March to October 2013. Patients were organized and analyzed according to gender, age, and results of the allergens subtypes tested. Results The study was composed of 1,912 patients (60% male and 40% female) of ages between 3 and 87 years. Positive tests were more prevalent in quantity and intensity with the mites Dermatophagoides pteronyssinus and Dermatophagoides farinae, each with 60% of the total analyzed. In second place were pollens, especially Dactylis glomerata and Festuca pratensis. Conclusion The female and male sexes were equally atopic. Fungi and epithelia of dog and cat were not considered potential aeroallergens that could cause symptoms. However, mites are common in Paraná, Brazil. Further studies regarding the pollens are needed, as this study result diverged from the literature. PMID:25992129

  6. HOUSE DUST MITE ALLERGY IN VIEW OF MOLECULAR ALLERGOLOGY

    Directory of Open Access Journals (Sweden)

    E. S. Korovkina

    2012-01-01

    Full Text Available Abstract. Incidence of allergic diseases is increased over last years. House dust is considered a major risk factor of allergic sensitization which plays an important role in development of allergic rhinitis, bronchial asthma, atopic dermatitis. Dust mites in the home comprise a large part of domestic allergens. Dermatophagoides pteronyssinus, Dermatophagoides farinae, Euroglyphus maynei, Lepidoglyphus destructor, Blomia tropicalis are most important in this respect. Der p 1 and Der p 2 are regarded as major house dust mite allergens. Recent studies concerned induction of IgE responses against mite tropomyosin (Der p 10, an allergen occurring in mites which exhibits high cross-reactivity with tropomyosins from a variety of sea foods (e.g. shrimps, as well as human tropomyosins. Allergen-specific immunotherapy (ASIT represents the only causative approach to allergy treatment in such cases. From this viewpoint, a quantitation of specific IgЕ against major house dust mite allergens would be necessary to predict ASIT efficiency. Treatment by house dust mites allergen extracts is effective in management of allergic rhinitis and mild asthma. A component-resolved diagnostic (CRD with purified house-dust mites allergens allows to discriminate patients who were mostly sensitized to the major house dust mites allergens (e.g. Der p 10, tropomyosin. The component-resolved diagnostics could be performed before starting the allergen-specific immunotherapy by mite allergens, in order to avoid unresponsiveness to this mode of therapy.

  7. The skin prick test – European standards

    Directory of Open Access Journals (Sweden)

    Heinzerling Lucie

    2013-02-01

    Full Text Available Abstract Skin prick testing is an essential test procedure to confirm sensitization in IgE-mediated allergic disease in subjects with rhinoconjunctivitis, asthma, urticaria, anapylaxis, atopic eczema and food and drug allergy. This manuscript reviews the available evidence including Medline and Embase searches, abstracts of international allergy meetings and position papers from the world allergy literature. The recommended method of prick testing includes the appropriate use of specific allergen extracts, positive and negative controls, interpretation of the tests after 15 – 20 minutes of application, with a positive result defined as a wheal ≥3 mm diameter. A standard prick test panel for Europe for inhalants is proposed and includes hazel (Corylus avellana, alder (Alnus incana, birch (Betula alba, plane (Platanus vulgaris, cypress (Cupressus sempervirens, grass mix (Poa pratensis, Dactilis glomerata, Lolium perenne, Phleum pratense, Festuca pratensis, Helictotrichon pretense, Olive (Olea europaea, mugwort (Artemisia vulgaris, ragweed (Ambrosia artemisiifolia, Alternaria alternata (tenuis, Cladosporium herbarum, Aspergillus fumigatus, Parietaria, cat, dog, Dermatophagoides pteronyssinus, Dermatophagoides farinae, and cockroach (Blatella germanica. Standardization of the skin test procedures and standard panels for different geographic locations are encouraged worldwide to permit better comparisons for diagnostic, clinical and research purposes.

  8. Which aeroallergens are associated with eczema severity?

    Science.gov (United States)

    Hon, K L E; Leung, T F; Lam, M C A; Wong, K Y; Chow, C M; Fok, T F; Ng, P C

    2007-07-01

    We investigated if a correlation exists between aeroallergen sensitization and the severity of eczema. Data on aeroallergen response to skin-prick testing (SPT) and disease severity of children with eczema (n = 119) were evaluated. Atopy, as defined by at least one positive response to aeroallergen skin prick testing, was found in > 90% of eczema patients. House dust mite was the most commonly sensitized aeroallergen, followed by cat fur. Dermatophagoides pteronyssinus and Dermatophagoides farinae sensitization were associated with eczema severity (present in 67% of the mild and 97% of the severe group; P = 0.001). However, there was no association between eczema severity and higher strengths of SPT response (defined as SPT > 1+ to dust mites or dust). Atopy to moulds, Bermuda grass, cockroach, cat and dog was less prevalent and was not associated with eczema severity. It is sensible to advise parents on specific avoidance strategies only in severely affected children who have a definitive history of eczema exacerbation by specific aeroallergens and who are not responsive to conventional treatment.

  9. The role of molds in the relation between indoor environment and atopy in asthma patients

    Directory of Open Access Journals (Sweden)

    Emel Ceylan

    2013-01-01

    Full Text Available Background: The effect of mold fungi to allergic sensitization is not well-known. We aimed to evaluate the role of molds in the relation between indoor environment and atopy in asthmatics. Materials and Methods: The air samples obtained from 66 stable asthmatics and 35 control subject′s houses were sprayed into Sabouraud dextrose agar. Allergy skin testing were performed in both groups. The temperature and humidity of each house were measured. Results: The incidence of atopy was similar in cases (59.1% and controls (51.4%. The average amount of mold was 35.9 CFU/m 3 and 34.3 CFU/m 3 , respectively. The number of household residents was positively correlated with the amount of molds. There was no difference in the amount of mold with respect to dosage of inhaler corticosteroids as well as symptom levels in asthmatics. The most frequently encountered allergens were Dermatophagoides farinae/Dermatophagoides pteronyssinus, grass/weeds and molds. Spending childhood in a village was more common among atopics. Conclusion: Living environment during the childhood might affect atopy and asthma. Based on the identification of molds as the second most frequent allergen after mites in our study population, assessment of mold sensitization as well as in forming patients about ways to avoid them seem likely to contribute to the effective management of uncontrolled asthma.

  10. The role of molds in the relation between indoor environment and atopy in asthma patients.

    Science.gov (United States)

    Ceylan, Emel; Doruk, Sibel; Genc, Sebahat; Ozkutuk, Ayşe Aydan; Karadag, Fisun; Ergor, Gul; Itil, Bahriye Oya; Cımrın, Arif Hikmet

    2013-12-01

    The effect of mold fungi to allergic sensitization is not well-known. We aimed to evaluate the role of molds in the relation between indoor environment and atopy in asthmatics. The air samples obtained from 66 stable asthmatics and 35 control subject's houses were sprayed into Sabouraud dextrose agar. Allergy skin testing were performed in both groups. The temperature and humidity of each house were measured. The incidence of atopy was similar in cases (59.1%) and controls (51.4%). The average amount of mold was 35.9 CFU/m(3) and 34.3 CFU/m(3), respectively. The number of household residents was positively correlated with the amount of molds. There was no difference in the amount of mold with respect to dosage of inhaler corticosteroids as well as symptom levels in asthmatics. The most frequently encountered allergens were Dermatophagoides farinae/Dermatophagoides pteronyssinus, grass/weeds and molds. Spending childhood in a village was more common among atopics. Living environment during the childhood might affect atopy and asthma. Based on the identification of molds as the second most frequent allergen after mites in our study population, assessment of mold sensitization as well as in forming patients about ways to avoid them seem likely to contribute to the effective management of uncontrolled asthma.

  11. Selective allergy to lobster in a case of primary sensitization to house dust mites.

    Science.gov (United States)

    Iparraguirre, A; Rodríguez-Pérez, R; Juste, S; Ledesma, A; Moneo, I; Caballero, M L

    2009-01-01

    Allergy to only 1 kind of seafood is uncommon. We report a case of selective allergy to lobster. We studied a 30-year-old man who suffered generalized urticaria, facial erythema, and pharyngeal pruritus after eating lobster. He had a more than 10-year history of mild persistent asthma and sensitization to house dust mites. The study was performed by skin prick test, and prick-prick test, oral food challenge, specific immunoglobulin (Ig) E determinations by CAP (Phadia, Uppsala, Sweden) and ADVIA-Centaur (ALK-Abelló, Madrid, Spain), and IgE-immunoblotting. The patient's serum recognized 2 allergens of around 198 kDa and 2 allergens of around 65 kDa from the lobster extract, allergens of around 15, 90, and 120 kDa from Dermatophagoides pteronyssinus extract, and allergens of around 15 and 65 kDa from Dermatophagoides farinae extract. Serum did not recognize purified shrimp tropomyosin. Immunoblot-inhibition assay results indicated cross-reactivity between lobster and mite allergens. This is the first report of selective allergy to lobster.

  12. A multicenter study of the clinical features of allergic rhinitis in central China.

    Science.gov (United States)

    Chen, Jianjun; Zhao, Yan; Li, Bin; Zhang, Quanming; Wan, Lijia; Liu, Jun; Tian, Xingde; Li, Guoyi; Zhou, Jie; Zhang, Long; Wang, Pengju; Cheng, Qi; Liang, Jianwei; Zhao, Ping; He, Jingpei; Lou, Zhihong; Lu, Haitao; Zhou, Yi; Zhang, Yuanfen; Chen, Xiangjun; Pan, Fangming; Zhang, Zhimao; Zeng, Ligang; He, Benchao; Zeng, Ying; Xu, Xiaoyan; Kong, Weijia

    2014-01-01

    Several epidemiological surveys of allergic rhinitis (AR) have been conducted in China. However, the clinical features of AR are still not clear enough. The aim of the current study was to perform a multicenter investigation to evaluate the clinical features of AR in China. A multicenter investigation was performed in 13 allergy centers in central China. A disease-related questionnaire was completed by each patient themselves or with guardian assistance after the diagnosis of AR. The clinical features of AR and allergen profile were analyzed. Eleven thousand four patients who were diagnosed with AR were recruited in this study. The percentages of classification of AR according to the Allergic Rhinitis and Its Impact on Asthma guidelines were 9.7% intermittent mild (IM), 3.1% persistent mild (PM), 33.9% intermittent moderate-severe (IMS), and 53.3% persistent moderate-severe (PMS). There were 61.6 and 42.2% AR patients who had concomitant ocular or lower respiratory symptoms in clinic. The occurrence of ocular and lower respiratory symptoms was found to be gradually increased from IM, PM, and IMS to PMS. Cold air and temperature change were the two most common factors triggering the nasal symptoms. Dermatophagoides pteronyssinus and Dermatophagoides farinae were the most important allergens of central China. This study has contributed to a better understanding of clinical features of AR in China.

  13. Sensitization Rates for Various Allergens in Children with Allergic Rhinitis in Qingdao, China.

    Science.gov (United States)

    Lin, Hang; Lin, Rongjun; Li, Na

    2015-09-07

    The aim of this study was to determine the prevalence of sensitization to common allergens in children with allergic rhinitis (AR) living in Qingdao, China. We conducted a retrospective analysis for AR cases, who underwent skin prick tests (SPT) in Qingdao. A total of 2841 children with AR qualified for the inclusion criteria (Age 3-5 years: 1500 children; Age 6-12 years: 1341 children). The most common inhaled allergens to which the AR children were sensitive were Dermatophagoides pteronyssinus (69.3%), Dermatophagoides farinae (66.2%) and mould 1 (Penicillium notatum 38.9%); while the corresponding ingested allergens were mussel (39.2%), shrimp (36.3%) and carp (36.5%). The prevalence of sensitization to inhaled allergens and food allergens was higher in children >6 years of age as compared to that in children 3-5 years of age (all p 6 years old were more sensitive to dust mite as compared to children 3-5 years old (p allergens causing AR in children in Qingdao, China. Older children with AR, particularly males, were found to be more sensitive to dust mite.

  14. Identification of astigmatid mites using ITS2 and COI regions.

    Science.gov (United States)

    Yang, Bin; Cai, Junlong; Cheng, Xunjia

    2011-02-01

    Identification of astigmatid mites based on their morphological characteristics is difficult because of the similarity of their organs, especially in immature mites. The ribosomal second internal transcribed spacer (ITS2) and the mitochondrial cytochrome oxidase subunit I (COI) regions are highly conserved in the eukaryotes and are usually used as barcodes. The ITS2 and COI regions of six species of astigmatid mites (Aleuroglyphus ovatus, Blomia tropicalis, Dermatophagoides farinae, Dermatophagoides pteronyssinus, Euroglyphus maynei, Tyrophagus putrescentiae) were obtained by polymerase chain reaction and sequenced. The lengths of the ITS2 sequences varied from 316 to 488 bp, while the COI regions were 377 or 378 bp long. Considering the ITS2 genes, the intraspecific genetic distance was in the range of 0.00-0.077844, whereas the interspecific genetic distance was 0.202426-0.912959. The values were 0.000-0.029748 and 0.138403-0.279304 for intra- and interspecific genetic distances when COI genes were used. The phylogenetic trees inferred from the ITS2 and the COI regions, by using maximum parsimony and neighbor-joining methods, were identical to those based on their morphological classification. Thus, the ITS2 and COI regions can be applied as barcodes to identify different species of astigmatid mites.

  15. 大连地区121例过敏性疾病患儿皮肤点刺试验结果分析%Analysis of allergens among 121 children with allergic diseases in Dalian

    Institute of Scientific and Technical Information of China (English)

    秦朋; 张琳; 李珊珊; 金玲; 涂彩霞

    2014-01-01

    目的:确定大连地区过敏性疾病患儿过敏原的种类。方法:对121例过敏性疾病患儿进行皮肤点刺试验(SPT)。结果:检测阳性率为76%(92例);居于前5位的过敏原为粉尘螨(47.9%)、屋尘螨(45.5%)、须发癣菌(29.8%)、霉菌 II(28.1%)和霉菌 I(24.8%)。结论:吸入性过敏原为大连地区过敏性疾病患儿的主要致敏原。%To determine allergens among 121 children with allergic diseases in Dalian. Methods: Skin prick test (SPT) was performed for 121 children with allergic diseases. Results: The positivity rate of the test was 76% (92 patients). The top five allergens were dermatophagoides farinae (47.9%), dermatophagoides pteronyssinus (45.5%), trichophyton mentagrophytes (29.8%), moulds II (28.1%) and moulds I (24.8%). Conclusion: Inhaling allergens are dominated in children with allergic diseases in Dalian.

  16. Cloning and sequence analysis of cDNA coding for group Ⅰ allergen of dermatophagoides farinae(Der f Ⅰ)%粉尘螨Ⅰ类变应原(Der fⅠ)的cDNA克隆及序列分析

    Institute of Scientific and Technical Information of China (English)

    郝敏麒; 徐军; 钟南山

    2001-01-01

    Objective To acquire the cDNA of group I allergen of (Der f Ⅰ) ofGuangzhou, China for further usage in construction of DNA vaccine and expression of the recombinant protein. Methods The live mites of local area which had been identified and cultured were picked. The total RNA was extracted. The Der f Ⅰ cDNA was amplified with RT-PCR. Then it was subcloned into a vector and sequenced. Results The segment with 632 bases was determined. The sequence homology with the published one (emb|X65196.1|)on gene bank was 99% at the nucleotide level.The deduced amino acid sequence homology was 100%. Conclusion It is the first time that we achieve the cDNA of Der f Ⅰ of Guangzhou. Its sequence homology is high as compared with the published one.%目的 获得我国广州地区粉尘螨Ⅰ类变应原(DerfⅠ)cDNA片段,为构建DNA疫苗或表达重组蛋白打下基础。方法 挑取经选择鉴定的活粉尘螨,提取总RNA,采用RT-PCR的方法扩增DerfⅠ片断,进行克隆、测序和分析。结果 获得长度为632个碱基对的核苷酸片断,序列分析结果和Genebank上去除内含子后的基因序列(emb|X65196.1|)同源性为99%,其中有6个碱基不同,推导的编码氨基酸序列同源性为100%。结论 我们首次获得广州地区的DerfⅠ的cDNA克隆,该克隆cDNA序列与Genebank上已公布的DerfⅠ序列高度同源。

  17. Experimental Study on the Der f 1 mRNA Molecules Derived from Dermatophagoides farinae for Specific Immunotherapy on Murine Model of Asthma%粉尘螨变应原Der f 1 mRNA对小鼠特异性免疫治疗的实验研究

    Institute of Scientific and Technical Information of China (English)

    姜玉新; 尹康; 靳文杰; 吴露依; 李朝品

    2014-01-01

    目的 探讨粉尘螨变应原Derf1mRNA疫苗对哮喘小鼠的特异性免疫治疗效果.方法 50只BALB/c小鼠随机分为5组(10只/组),分别为PBS组、Derf1变应原致敏组、Derf1变应原免疫治疗组、β-actin mRNA免疫治疗组和Derf1 mRNA免疫治疗组.分别于第0、第7和第14天,PBS组小鼠腹腔注射PBS,其余4组小鼠则腹腔注射10 μgDer f 1进行致敏,建立小鼠哮喘模型.自第21天起,除PBS组小鼠雾化吸入PBS,其他4组小鼠均雾化吸入100 μg/mlDerf1变应原,30 min/次,1次/d,连续7d,观察并记录小鼠哮喘发作情况.5组小鼠于最后1次雾化吸入致敏2周后,背部皮下分别注射100 μl PBS、1μg Derf1(维持致敏)、10 μg Derf1(免疫治疗)、2μg β-actin mRNA和2μg Derf1mRNA,1次/周,连续3周.最后1次皮下注射后2周处死小鼠.收集各组小鼠的支气管肺泡灌洗液(BALF),ELISA法检测γ干扰素(IFN-γ)和白介素13 (IL-13)水平,并计数嗜酸粒细胞(EOS);收集各组小鼠脾组织,分离脾细胞,除PBS组外,其他4组均加入10 μg/ml Der f 1培养72 h,ELISA法检测脾细胞培养上清液中IFN-γ和IL-13水平;取各组小鼠眼球血,ELISA法检测血清中总IgE以及变应原特异性IgE (sIgE)、IgG1(sIgG1)和IgG2a (sIgG2a)抗体水平.HE染色观察各组小鼠肺组织切片. 结果 除PBS组外,其他4组小鼠雾化吸入致敏后,均出现急性哮喘发作症状.小鼠BALF中,Derf 1 mRNA免疫治疗组和Derf 1免疫治疗组的IFN-γ水平分别为(897.56±105.73)和(864.48±70.62) pg/ml,均明显高于Derf 1变应原致敏组[(209.05±52.28) pg/ml]和β-actin mRNA免疫治疗组[(219.47±64.72) pg/ml](P<0.01);两者IL-13水平分别为(241.64±31.41)和(321.94±41.07) pg/ml,则明显低于Derf1变应原致敏组[(520.62±43.77) pg/ml]和β-actin mRNA免疫治疗组[(507.22±42.26) pg/ml] (P<0.01);两者EOS数量分别为(1.33±0.44) ×105和(1.48±0.39) ×105个/ml,均明显低于Derf1变应原致敏组[(3.54±0.52) ×105个/ml]和β-actin mRNA免疫治疗组[(2.98±0.53)×105 个/ml](P<0.01).脾细胞培养上清的ELISA检测结果显示,Derf 1 mRNA免疫治疗组和Derf 1免疫治疗组的IFN-γ水平分别为(420.91±69.92)和(334.92±43.72) pg/ml,明显高于Derf1变应原致敏组[(123.75±15.48) pg/ml]和β-actin mRNA免疫治疗组[(128.84±59.00) pg/ml](P<0.01);两者IL-13水平[(268.51±40.42)和[(285.26±62.21) pg/ml]则显著低于Derf1变应原致敏组[(613.89.±.51.54) pg/ml]和β-actin mRNA免疫治疗组[(524.05±39.12) pg/ml] (P<0.01).血清中抗体水平的ELISA检测结果显示,Derf 1 mRNA免疫治疗组的总IgE、sIgE和sIgG1抗体水平分别为(33.72±9.78)、(22.76±8.09)和(17.87±7.59) ng/ml,均显著低于Derf1变应原致敏组[(94.34±11.66)、(65.67±9.47)和(75.18±9.52)ng/ml]和β-actin mRNA免疫治疗组[(86.48±10.26)、(62.36±8.35)和(69.51±8.98) ng/ml] (P<0.01);其sIgG2a抗体水平为(7.74±0.88) ng/ml,则明显高于Derf1变应原致敏组[(2.81±1.17) ng/ml]和β-actin mRNA免疫治疗组[(1.06±0.11) ng/ml](P<0.01).肺组织切片HE染色镜检结果显示,与Derf1变应原致敏组相比,Derf1 mRNA免疫治疗组小鼠的气道上皮和肺泡上皮细胞结构基本完整,炎症细胞浸润明显减少.结论 Derf1mRNA疫苗可有效纠正Th1/Tn2失衡.

  18. Construction and expression of the recombinant plasmid pET28a-YARA-Ihc-ProDer f 1 of Derma-tophagoides farina%粉尘螨变应原ProDerf1编码基因重组pET28a-YARA-Ihc体系的构建及表达

    Institute of Scientific and Technical Information of China (English)

    刘志明; 姜玉新

    2014-01-01

    Objective To construct the prokaryotic expression vector pET28a-YARA-Ihc-ProDer f 1 and provide the foundation for exploring the fusion protein effect as vaccine for specific immunotherapy. Meth-ods Two oligonucleotides encoding YARA were synthesized and annealed to generate YARA-encoding DNA. The fused genes, YARA-Ihc-ProDer f 1 was constructed and inserted into the prokaryotic expression vector pET28a(+). The fusion protein YARA-Ihc-ProDer f 1 induced with IPTG in E.coli BL21(DE3) was purified with Ni2+-resin affinity chromatography and confirmed with SDS-PAGE and Western blot. Results Sequence analysis confirmed the construction of the expression vector pEt28a-YARA-Ihc-ProDer f 1 success-fully, the fusion protein YARA-Ihc-ProDer f 1 was expressed and purified with the concentration of 278μg/ml. SDS-PAGE and Western blot demonstrated the fusion protein was YARA-Ihc-ProDer f 1. Conclusion The recombinant prokaryotic expression vectors, pET28a(+)-YARA-Ihc-ProDer f 1 was successfully con-structed, and the fusion protein was expressed and purified .%目的:构建粉尘螨变应原ProDer f 1原核表达载体pET28a-YARA-IhC-ProDer f 1,为评价其免疫治疗效果奠定基础。方法用分子克隆技术构建出表达载体pET28a-YARA-IhC-ProDer f 1,在E.coli BL21(DE3)中表达融合蛋白YARA-IhC-ProDer f 1,并进行Ni2+-NTA树脂柱亲和层析以纯化蛋白。结果经测序证实成功构建了表达载体pET28a-YARA-IhC-ProDer f 1,YARA-IhC-ProDer f 1融合蛋白在E.coli BL21(DE3)中得到表达,纯化后的蛋白浓度为278μg/ml。SDS-PAGE和Western blot分析表明纯化蛋白为目的蛋白YARA-IhC-ProDer f 1。结论已成功制备出pET28a-YARA-IhC-ProD-er f 1原核表达载体,融合蛋白得到表达和纯化。

  19. Effect of Age on Allergen Responses of Allergic Patients in Southern Taiwan

    Directory of Open Access Journals (Sweden)

    Wen Chiang

    2004-07-01

    Full Text Available To survey airborne and food allergen patterns in southern Taiwan and to analyze the effect of age on response to different allergens, we tested samples from 4,411 allergic patients at Kaohsiung Medical University Hospital using the MAST-CLA test (new Taiwan panel. A total of 2,212 (50.1% samples showed a positive response. We grouped allergic patients into five age groups. Milk and egg white were the main food allergens in the younger groups (< 3 years old and 3-6 years old. Shrimp, crab, and shellfish were the main allergens in the groups aged 7-12, 13-18, and more than 18 years. Among airborne allergens, house dust and mites Dermatophagoides farinae and D. pteronyssinus were the main allergens in all age groups, whereas the frequency of response to cockroach allergen was low in the group aged less than 3 years, but increased in the other age groups. There was a sharp increase in the frequency of response to airborne allergens after 3 years old and a sharp decrease in response to food allergens. Among subjects allergic to both airborne and food allergens, there was a positive MAST-CLA rate of 19.9% to 26% (all five age groups, no significant difference. When we compared our results with those from Taipei Veterans General Hospital in northern Taiwan, there were significant differences for yeast, peanut, feather mix, dog dander, cockroach, D. farinae and D. pteronyssinus allergens (p < 0.01. These differences were probably caused by differences in patient location, patient age, disease patterns and allergen panels.

  20. Comparación de la potencia de extractos alergénicos de ácaros en pacientes con asma y rinitis alérgica

    Directory of Open Access Journals (Sweden)

    Olimpio Rodríguez

    2012-04-01

    Full Text Available Las pruebas cutáneas para el diagnóstico específico en alergia requieren de extractos alergénicos de óptima calidad, que pueden ser comprobadas determinando la potencia alergénica con el empleo de distintas concentraciones de extractos de un fabricante o confrontando los de diferentes laboratorios. Con el objetivo de comparar extractos de ácaros: Dermatophagoides pteronyssinus, D. farinae, D. siboney y Blomia tropicalis , producidos en los laboratorios de DIATER, Argentina, ALK-Abelló, España y BIOCEN, Cuba, se realizó la prueba a 56 pacientes entre 2 y 15 años, con asma y rinitis, mediante la respuesta cutánea al Prick Test, en el Policlínico Previsora, de la ciudad de Camagüey. El 84,6% de los pacientes asociaron los síntomas alérgicos a cambios de tiempo y exposición a polvo casero. El diámetro de los habones fue aparentemente mayor para ALK-Abelló, pero sin diferencia significativa. Se apreció gráficamente una tendencia lineal entre el diámetro promedio del habón y el logaritmo de la concentración de alergeno cuando se emplearon diferentes concentraciones, en el rango de 4.000 a 100.000 UB/mL. Los Prick Test, con diámetro ³ 3 mm, mostraron una aparente mayor proporción para ALK-Abelló con D. pteronyssinus y D. farinae , en comparación con BIOCEN y DIATER, aunque tampoco de forma significativa. Los extractos de ácaros, producidos y estandarizados en DIATER, BIOCEN y ALK-Abelló, son similares en cuanto a sus potencias alergénicas.

  1. Sero-prevalence study of IgE responses to allergens from Malaysian house dust (HDM) and storage mites (SM).

    Science.gov (United States)

    Chong, K T; Wong, S F; Mak, J W; Loh, L C; Ho, T M

    2015-09-01

    Allergens of Dermatophagoides and Blomia species are well-characterized but not for other species. This study was conducted to determine the prevalence of allergic sensitization to house dust (HDM) and storage mites (SM). One hundred adult subjects (aged ≥ 18) were recruited. The mite specific IgE of all allergic subjects were higher compared with healthy subjetcs despite being not statistically significant except for D. farinae and G. malaysiensis. The mean serum IgE levels against HDM and SM for allergic subjects were significantly higher compared with those in healthy subjects. They were mainly sensitized to Dermatophagoides farinae (35%) and Glycycometus malaysiensis (37%). Immunoblots revealed not all allergic subjects showed positive immuno-reactivity against the mites tested. Single or multiple bands were observed for different species. The subjects were commonly sensitized to Group 2 (9-12 kDa), 10 (38 kDa) and 18 (40-48 kDa) allergens. Twenty-one out of 60 allergic subjects were sensitized to either one or more species. The majority of them (71%) were sensitized to single species. The allergic subjects were mainly sensitized to D. pteronyssinus, followed by Tyrophagus putrecentiae and Aleuroglyphus ovatus. Seven were solely sensitized to HDM while 10 were solely sensitized to SM. Four subjects were sensitized to both. Pre-adsorption study revealed no cross-reactivity. There was difference between the prevalence and reactivity to allergens of HDM and SM in these subjects. Both ELISA and immunoblot did not correlate well but can complement each other in improving the detection of mite allergens to the species level.

  2. Domestic mite antigens in floor and airborne dust at workplaces in comparison to living areas: a new immunoassay to assess personal airborne allergen exposure.

    Directory of Open Access Journals (Sweden)

    Ingrid Sander

    Full Text Available OBJECTIVES: Allergens produced by domestic mites (DM are among the most common allergic sensitizers and risk factors for asthma. To compare exposure levels between workplaces and living areas a new assay able to measure airborne DM antigen concentrations was developed. METHODS: At workplaces and in living areas, 213 floor dust samples and 92 personal inhalable dust samples were collected. For sensitive quantification of DM antigens, a new enzyme immunoassay (EIA based on polyclonal antibodies to Dermatophagoides farinae extract was developed. Reactivity of five house dust mite and four storage mite species was tested. All dust samples were tested with the new EIA and with the Der f 1 and Der p 1-EIAs (Indoor Biotechnologies, UK which detect major allergens from D. farinae and D. pteronyssinus by monoclonal antibodies. Samples below the detection limit in the DM-EIA were retested in an assay variant with a fluorogenic substrate (DM-FEIA. RESULTS: The newly developed DM-EIA detects antigens from all nine tested domestic mite species. It has a lower detection limit of 200 pg/ml of D.farinae protein, compared to 50 pg/ml for the DM-FEIA. DM antigens were detected by DM-EIA/FEIA in all floor dust and 80 (87% of airborne samples. Der f 1 was found in 133 (62% floor dust and in only 6 airborne samples, Der p 1 was found in 70 (33% of floor samples and in one airborne sample. Der f 1 and DM concentrations were highly correlated. DM-antigens were significantly higher in inhalable airborne samples from textile recycling, bed feather filling, feed production, grain storage and cattle stables in comparison to living areas. CONCLUSIONS: A new sensitive EIA directed at DM antigens was developed. DM antigen quantities were well correlated to Der f 1 values and were measurable in the majority (87% of airborne dust samples. Some workplaces had significantly higher DM antigen concentrations than living areas.

  3. House Dust Mite Prevalence in the House of Patients with Atopic Dermatitis in Mashhad, Iran

    Directory of Open Access Journals (Sweden)

    Toktam Ziyaei

    2017-06-01

    Full Text Available Background: Being exposed to house dust mites intensifies atopic dermatitis. This study has investigated the con­tamination rate with Dermatophagoides mites in patient's residential home with atopic dermatitis.Methods: In this cross-sectional study, 40 patients took part with atopic dermatitis (positive or negative for mites by prick Dermal Test. Samples were collected from 3 locations (living room, bedroom and bed by vacuum cleaner. Dust samples (transferred to freezer -20 ˚C were examined by direct method and flotation. The data were analyzed using statistical SPSS vr.20 software.Results: Twenty patients of positive prick test included 8 (40% male and 12 (60% female. The results of direct observation of mites: 7 cases (35% in bedding sheets, 6 cases (30% bedrooms' carpet, 3 cases (15% living room's carpet. Twenty patients of negative prick test included 8 (40% male and 12 (60% female. Only mites were found (5% in living room's carpets of negative prick test patients. Dermatophagoides pteronyssinus was more frequent than Dermatophagoides farinae. (98% vs 83%.Conclusion: Fifty-five percent of residential homes of prick test positive patients and only 5% of residential homes of prick test negative patients were positive for mite. Sunshine provided home had fewer mites than home where sunshine is not provided. Prick test positive patients used handmade carpets more than machine made ones. In posi­tive prick test patients, mites were found in bed sheet and bedroom’s carpet more than negative prick test patient's sheets and carpets.

  4. [Skin reactivity frequency to aeroallergens in patients with clinical symptoms of allergic disease].

    Science.gov (United States)

    Rojas-Méndez, Isabel Cristina; Arana-Muñoz, Oswaldo; López-García, Aída Inés; Rivero-Yeverino, Daniela; Caballero-López, Chrystopherson Gengyny; Papaqui-Tapia, Sergio; Camero-Martínez, Heriberto; Vázquez-Rojas, Elizabeth

    2017-01-01

    Antecedentes: El diagnóstico de las enfermedades alérgicas debe basarse en la historia clínica alergológica adecuada y en una prueba inmunológica de sensibilización; la de mayor sensibilidad y especificidad es la prueba cutánea por punción. Objetivo: Determinar la frecuencia de la reactividad cutánea hacia aeroalérgenos, por grupos etarios, en pacientes del Servicio de Alergia e Inmunología Clínica del Hospital Universitario de Puebla, México. Métodos: Se realizó estudio transversal que incluyó a pacientes de 2 a 64 años de edad, con síntomas sugestivos de enfermedad alérgica, en quienes se realizaron pruebas cutáneas con aeroalérgenos; los criterios diagnósticos fueron los de las guías internacionales. Se calcularon frecuencias, porcentajes y medidas de dispersión. Resultados: De 173 pacientes, 63 % fue del sexo femenino. La edad media fue de 22.3 años. La frecuencia de la reactividad cutánea para Quercus sp. fue 12.72 %, Periplaneta americana 9.83 %, Dermatophagoides farinae 9.25 %, Cynodon dactylon 8.09 %, Blatella germanica 8.09 %, Holcus halepensis 6.94 %, Dermatophagoides pteronyssinus 6.36 %, Schinus molle 5.78 %, Fraxinus uhdei 5.20 %, Lolium perenne 5.20 %, Ambrosia eliator 5.20 % y Artemisa tridentata 4.62 %. Conclusión: Los Dermatophagoides son los aeroalérgenos más identificados, pero en el presente estudio fue más común un polen, probablemente debido a factores geográficos-medioambientales, aunque no fue así en el análisis por grupos etarios.

  5. [Safety of subcutaneous immunotherapy with tyrosine-adsorbed house dust mite extracts in patients with allergic disease].

    Science.gov (United States)

    Molina-Sáenz, Mónica María; Villa-Arango, Ana María; Cardona-Villa, Ricardo

    2017-01-01

    Antecedentes: Pese a los múltiples beneficios de la inmunoterapia alérgeno-específica, en algunos países se restringe su uso por temor a las reacciones adversas severas. Objetivo: Evaluar las reacciones sistémicas adversas en pacientes con dermatitis atópica, asma, rinitis y conjuntivitis alérgicas, que recibieron inmunoterapia subcutánea con extractos tirosinados para ácaros Dermatophagoides y Glycyphagoides. Métodos: Estudio retrospectivo del periodo 2010-2015 en el que se incluyó a 773 pacientes con diagnóstico de enfermedades mediadas por IgE. Se describió la seguridad de la inmunoterapia alérgeno-específica conforme al sistema de clasificación de las reacciones sistémicas con inmunoterapias subcutánea de la Organización Mundial de Alergias. Resultados: 79.7 % de los pacientes presentó rinitis, 54.9 % asma, 34.5 % conjuntivitis y 16.4 % dermatitis atópica. De 12 546 dosis subcutánea con extractos tirosinados se registraron 45 reacciones sistémicas: 12 grado 1 (30 %), 27 de grado 2 (67.5 %) y 1 grado 3 (2.5 %); la tasa de reacción fue de 0.35 por cada 100 inyecciones administradas, que representó una incidencia de 5.8 %. No se registraron reacciones fatales. Conclusión: Con la inmunoterapia subcutánea con extractos tirosinados de Dermatophagoides farinae, Dermatophagoides pteronyssinus y Blomia tropicalis se presentó una frecuencia de reacciones sistémicas similar a la informada con otros extractos.

  6. 儿童体外过敏原的检测及其结果分析%In vitro allergen testing and result analysis in children

    Institute of Scientific and Technical Information of China (English)

    方娴静; 郑岚; 徐种; 傅启华

    2009-01-01

    目的 研究上海市儿童常见过敏原的致敏情况,为儿童过敏性疾病的防治提供科学依据.方法 应用Mediwiss公司出品的AllergyScreen(敏筛)过敏原检测系统对671例患儿进行常见的吸入性和食入性过敏原体外免疫测试,并计算各种过敏的所占阳性比.结果 吸人性过敏原中以尘螨(粉尘螨和户尘螨)和Mxl点青霉分枝孢子为主,分别占阳性病例的44.75%和26.57%,其中31.23%和6.76%反应级数为3~6级,呈强阳性;其他依次为猫毛皮屑、狗毛皮屑、树花粉组合、蟑螂等.食入性过敏原中以鸡蛋和牛奶为主,分别占阳性病例的34.04%和18.46%,其中5.14%和3.18%反应级数为3~6级,呈强阳性;其他依次为花生、黄豆、腰果,牛肉、羊肉,小麦等.结论 在上海市,粉尘螨、户尘螨和Mxl点青霉分枝孢子是主要的儿童吸人性过敏原,鸡蛋和牛奶是主要的儿童食入性过敏原,总体上儿童食人性过敏原阳性率略高于吸入性过敏原,但反应强度低于吸人性过敏原.开展儿童体外特异性过敏原检测有助于临床诊断过敏性疾病.%Objective To investigate common allergens causing children allergic diseases in Shanghai in order to provide a scientific evidence in the preventing and treatment of children allergic diseases. Methods Using automatic in vitro immunity diagnosis meter (AllergySereen), some common kinds of inhalation allergens and food allergens were detected in 671 children allergy cases. The positive rate of each kind of allergen was calculated. Results Dermatophagoides farinae/dermatophagoides pteronyssinus and combination of Streptomyces were the main inhalation allergens and the positive rates of them were 44.75% and 26.57%, respectively, which 31.23% and 6.76% were 3-6 reaction order, and the rates showed strong positive. Other common allergens were cat's and dog's hair, combination of tree/flower's farina and cockroaches. The main food allergens were eggs and milk and

  7. Testes cutâneos de hipersensibilidade imediata com o evoluir da idade Positive skin test and age

    Directory of Open Access Journals (Sweden)

    Wilma Carvalho Neves Forte

    2001-04-01

    Full Text Available OBJETIVO: avaliação da positividade aos testes cutâneos de hipersensibilidade imediata em crianças com asma brônquica e/ou rinite alérgica em diferentes faixas etárias. CASUÍSTICA E MÉTODOS: foi observada a positividade aos testes cutâneos de hipersensibilidade imediata, por testes de puntura, frente a diferentes alérgenos de mesma procedência: poeira total e Dermatophagóides sp, Dermatophagoides pteronyssinus, Dermatophagoides farinae e Blomia tropicalis, Penicillium sp, Alternaria alternata, Cladosporium herbarium, Aspergillus fumigatus, grama bermuda, capim de pasto, epitélio de cão, epitélio de gato, penas, Blatella germanica, lã. Foram selecionadas 713 crianças divididas em grupos conforme a faixa etária: grupo I (6 a 11 meses, II (1 a 3 anos e 11 meses, III (4 a 8 anos e 11 meses e IV (9 a 15 anos. Para análise estatística utilizou-se o cálculo do qui-quadrado. RESULTADOS: o total de diferenças significativas entre os vários grupos foi: I e II = 5; II e III = 5; II e IV = 5; III e IV = 6; I e III = 10 e I e IV = 10 CONCLUSÃO: concluiu-se que a positividade ao teste de hipersensibilidade imediata foi maior com o evoluir da idade, havendo positividade já aos doze meses de vida, sendo esta positividade significativamente maior a partir de quatro anos de idade.OBJECTIVE: to evaluate positive responses to skin tests for immediate hypersensitivity to allergens in children with asthma and rhinitis at different ages. METHOD: we observed positive skin test reactivity in prick tests using fifteen allergens of same origin (total dust and Dermatophagoides sp.; Dermatophagoides pteronyssinus; Dermatophagoides farinae; Blomia tropicalis; Penicillium sp; Alternaria alternata; Cladosporium herbarium; Aspergillus fumigatus; Bermuda grass; forage grass; dog and cat epithelia; feathers; Blatella germanica and wool. We placed 713 selected patients into different age groups - Group I: 6 to 11 months; Group II: 1 to 3 years and 11

  8. Clinical analysis of the skin prick test in Shanghai area%上海地区过敏患者皮肤点刺试验临床分析

    Institute of Scientific and Technical Information of China (English)

    孙立英; 郭胤仕; 王菁兰; 王怡玮; 许以平

    2011-01-01

    To understand the predominant allergens for allergic diseases existed in Shanghai area, distributions and changes of these allergens, 1 407 allergic patients underwent skin prick test(SPT) were entered into this study.It was showed that 5 most encountered allergens with positive reactions were Dermatophagoides farinae (64.89 %), Dermatophagoides pteronyssinus (58.56 % ), Blomia tropicalis (40.58 %), Blattella germanica (21.32 % ) and Periplaneta americana (20.90 % ).House dust mites, such as (D.farinae, D.pteronyssinus and Blattella germanica) demonstrated the highest positive rate.Patients allergic to house dust mites(D.farinae, D.pteronyssinus and Blattella germanica ), cockroaches(Blattella germanica and Periplaneta americana), dog dander, cat dander and shrimp were all age-related.The positive rate to house dust mites,cockroaches, dog dander, cat dander and pollens in patients with respiratory allergic diseases was significantly higher than in patients with allergic skin disease(P<0.05).These data indicate that house dust mites are the predominant allergens for allergic diseases in Shanghai area, and patients allergic to house dust mites, cockroaches, dog dander, cat dander and shrimp are age-related, but unrelated to sex.Patients with respiratory allergic disease had a higher positive rate than patients with allergic skin diseases.%为了解上海地区过敏性疾病的主要过敏原,探讨过敏患者过敏原的分布情况及变化规律,收集1 407例过敏患者的皮肤点刺试验资料.结果显示,阳性率位于前五位的过敏原依次为粉尘螨64.89%、屋尘螨58.56%、热带螨40.58%、德国小蠊21.32%和美洲大蠊20.90%.以尘螨(粉尘螨、屋尘螨和热带螨)的阳性率最高.过敏患者对尘螨(粉尘螨、屋尘螨和热带螨)、蟑螂(德国小蠊和美洲大蠊)、狗毛屑、猫毛屑及虾过敏与年龄有关.呼吸道过敏性疾病患者对尘螨、蟑螂、狗毛屑、猫毛屑和

  9. Perfil de sensibilização a alérgenos domiciliares em pacientes ambulatoriais Indoor allergen sensitization profile in allergic patients of the allergy clinic in the University Hospital in Uberlândia, Brazil

    Directory of Open Access Journals (Sweden)

    Fernanda Aparecida Almeida Soares

    2007-02-01

    Full Text Available OBJETIVO: Conhecer o perfil de sensibilização dos pacientes com diagnóstico de doenças alérgicas atendidos no Ambulatório de Alergia do Hospital de Clínicas da Universidade Federal de Uberlândia. MÉTODOS: Foi realizado um estudo retrospectivo por meio da análise de prontuários de pacientes atendidos no serviço, que foram submetidos ao teste cutâneo de puntura (TCP para os alérgenos de Dermatophagoides pteronyssinus (Der p, Dermatophagoides farinae (Der f, Blomia tropicalis (Blo t, Canis familiaris (Can f, Felis domesticus (Fel d, Blattella germanica (Bla g e Alternaria alternata, e respondido corretamente ao questionário ISAAC. RESULTADOS: Foram analisados 212 prontuários de pacientes que preenchiam os critérios de inclusão. A rinite alérgica isoladamente foi a maior causa de atendimento (32%, seguida das associações asma e rinite (29,7% e asma, rinite e eczema (9,4%. Pacientes com asma isoladamente perfizeram apenas 1,9% dos atendimentos. A sensibilização dos pacientes observada foi de 73,5%, sendo de 61,7% para Der p, 59,9% para Der f, 54,7% para Blo t, 45,7% para Bla g, 38,2% para Can f, 33,3% para Fel d e 9,9% para Alternaria. Não houve diferenças significantes entre as patologias encontradas e os perfis de sensibilização. CONCLUSÃO: Os principais alérgenos sensibilizantes determinados pelo TCP foram os ácaros, com predomínio de Der p e Der f, chamando a atenção a elevada prevalência de sensibilização ao último. Ainda foi observada elevada sensibilização aos alérgenos de B. germanica, superior aos estudos anteriores realizados no País.OBJECTIVE: To evaluate allergens among patients with allergic respiratory disease attended at the Allergy Clinic of the University Hospital - Federal University of Uberlândia. METHODS: A retrospective study was performed using medical records. Patients were included if their ISAAC questionnaires were correctly filled out and their skin prick tests were positive to at

  10. Analysis of inhaled allergens in the patients with chronic rhinosinusitis in Hainan province%海南地区慢性鼻-鼻窦炎患者吸入性变应原血清检测结果分析

    Institute of Scientific and Technical Information of China (English)

    王晓凤; 周学军; 苏炳泽; 冯勇军; 郑少江; 冼德生; 黄家军; 刘世喜

    2014-01-01

    目的:通过检测海南地区慢性鼻-鼻窦炎(CRS)患者血清变应原,了解海南地区主要吸入性变应原分布情况,探讨有效实施变应原避免的措施,为成功开展特异性免疫治疗提供依据。方法:用UniCAP100全自动变应原检测仪对318例 CRS 患者进行吸入物变应原过筛试验(Phadiatop)并检测7种常见变应原的血清特异性IgE(specific IgE,SIgE)水平,同时检测其血清总IgE(total IgE,TIgE)水平。结果:血清TIgE阳性率为64.15%,吸入变应原过筛实验阳性率为37.74%。SIgE阳性的总发生率为33.96%。其中户尘螨(28.30%)、热带螨(27.36%)、粉尘螨(21.07%)、蟑螂(13.52%)、屋尘(11.64%)、猫皮屑(7.86%)、狗皮屑(0.63%)。在伴鼻息肉的CRS和不伴鼻息肉的CRS之间,TIgE 阳性及SIgE 阳性的发生率比较差异均无统计学意义。结论:户尘螨、热带螨、粉尘螨是海南地区CRS患者主要的吸入性变应原。%Objective To understand the distribution of inhaled allergens throughout Hainan province and explore effective preventive measures against allergen by examining the serum allergen of patients with chronic rhinosinusitis (CRS), which will provide evidence for specific immunotherapy for treating CRS. Methods Three hundred and eighteen CRS patients underwent Phadiatop blood test by using the UniCAP 100 , a completely automatic autoanalyser. Allergen-specific IgE of 7 common allergens were tested and the concentration of total immunoglobulin E (TIgE) was collected and evaluated. Results The positive rates of the serum TIgE and inhaled allergens were 64.15% and 37.74% respectively. The incidences of the positive serum SIgE is 33.96%. Among the positive cases, 28.30% of the inhaled allergens were dermatophagoides pteronyssinus, 27.36% for tropical mites, 21.07% for dermatophagoides farinae, 13.52% for cockroach, 11. 64% for house dust, 7

  11. Bartonella-like bacteria carried by domestic mite species.

    Science.gov (United States)

    Kopecký, Jan; Nesvorná, Marta; Hubert, Jan

    2014-01-01

    Bacteria of the genus Bartonella are carried by haematophagous mites, ticks, fleas and flies, and attack the erythrocytes of mammals. Here we describe a Bartonella-like clade, a distinct group related to Bartonellaceae, in stored-product mites (Acari: Astigmata) and a predatory mite Cheyletus eruditus (Acari: Prostigmata) based on the analysis of cloned 16S rRNA gene sequences. By using the clade-specific primers, closely related Bartonella-like 16S rRNA sequences were amplified from both laboratory colonies and field strains of three synanthropic mite species (Acarus siro, Lepidoglyphus destructor and Tyrophagus putrescentiae) and a predatory mite. Altogether, sequences of Bartonella-like bacteria were found in 11 strains, but were not detected in Dermatophagoides farinae and D. pteronyssinus and two strains of L. destructor. All obtained sequences formed a separate cluster branching as a sister group to Bartonellaceae and related to other separate clusters comprising uncultured bacterial clones from human skin and hemipteran insects (Nysius plebeius and Nysius sp.). The classification of sequences into operational taxonomic units (OTUs) showed a difference between A. siro and T. putrescentiae suggesting that the Bartonella-like bacteria are different in these two mite species. However, species specific sequences in separate OTUs were observed also for C. eruditus. Possible symbiotic interactions between Bartonella-like bacteria and their mite hosts are discussed.

  12. Mites and allergy.

    Science.gov (United States)

    Fernández-Caldas, Enrique; Puerta, Leonardo; Caraballo, Luis

    2014-01-01

    Allergic diseases triggered by mite allergens include allergic rhinoconjunctivitis, asthma, atopic dermatitis and other skin diseases. Since the early discovery of the allergenic role of mites of the genus Dermatophagoides in the mid 1960s, numerous species have been described as the source of allergens capable of sensitizing and inducing allergic symptoms in sensitized and genetically predisposed individuals. The main sources of allergens in house dust worldwide are the fecal pellets of the mite species D. pteronyssinus, D. farinae, Euroglyphus maynei and the storage mites Blomia tropicalis, Lepidoglyphus destructor and Tyropahgus putrescentiae. Group 1 and 2 allergens are major house dust mite allergens. The main allergens in storage mites include fatty acid-binding proteins, tropomyosin and paramyosin homologues, apolipophorin-like proteins, α-tubulins and others, such as group 2, 5 and 7 allergens. Cross-reactivity is an important and common immunological feature among mites. Currently, purified native or recombinant allergens, epitope mapping, proteomic approaches and T cell proliferation techniques are being used to assess cross-reactivity. Mites contain potent enzymes capable of degrading a wide range of substrates. Most mite allergens are enzymes. Advances in genomics and molecular biology will improve our ability to understand the genetics of specific IgE responses to mites. Mite allergen avoidance and immunotherapy are the only two allergen-specific ways to treat mite-induced respiratory and cutaneous diseases. © 2014 S. Karger AG, Basel.

  13. [Evaluation of allergen-specific IgE antibodies by a newly developed mast allergy system].

    Science.gov (United States)

    Nakagawa, T; Iwasaki, E; Baba, M; Matsushita, T; Baba, S; Ito, K; Miyamoto, T

    1989-06-01

    MAST, which stand for multiple antigen simultaneous test, uses enzyme-linked anti-human IgE and chemiluminogenic substate to determine IgE. This system is characterized by simultaneous analysis of multiple allergen items, up to 35, together with total IgE determination. We evaluated usefulness of this MAST system using 191 serum samples obtained from patients with bronchial asthma, allergic rhinitis and/or atopic dermatitis. It was found that there were statistically significant correlations between IgE antibody quantification by MAST and RAST in 24 out of 35 allergen items, with correlation coefficients more than r = 0.60. These included Dermatophagoides farinae and pteronyssinus, Japanese cedar pollen, orchard grass, Alternaria, Candida as inhalant allergens; egg white, milk, soybeans, wheat and rice as food allergens. It was also evaluated how many allergen-specific IgE antibodies could be detected in one serum sample. More than six allergen-specific IgE antibodies were simultaneously detected in 33% of 191 cases, indicating the importance of multiple-allergen analysis. These results indicate the clinical usefulness of the MAST allergy system in detecting IgE antibodies in allergic subjects.

  14. A 15-Year-Old Boy with Anterior Chest Pain, Progressive Dyspnea, and Subcutaneous Emphysema of the Neck

    Directory of Open Access Journals (Sweden)

    Nicola Scichilone

    2009-01-01

    Full Text Available We describe the case of an adolescent who was admitted to the hospital because of sudden occurrence of chest pain, dyspnea and subcutaneous emphysema. On admission, physical examination revealed subcutaneous crepitations in the superior part of the rib cage, and auscultation of the chest showed widespread wheezing. The radiological assessment confirmed the diagnosis of pneumomediastinum and pneumothorax. A follow-up CT scan performed one week after the admission showed almost complete resolution of the radiological alterations. At the following visits, the patient was asymptomatic, but reported to have suffered from frequent episodes of rhinorrea, sneezing, nasal blockage, and sometimes, chest tightness, especially during exposure to pets and/or windy weather. Skin prick testing showed sensitivities to dermatophagoides pteronyssinus and farinae, grass pollen and dog dander. Spirometry documented significant improvement in lung function after short-acting bronchodilator, allowing for the diagnosis of asthma to be made. Although pneumomediastinum may be a complication of various respiratory diseases, including asthma, it has never been reported as the first presentation of underlying bronchial asthma. Herein, the physiopathological mechanisms, the diagnostic procedures and treatment of pneumomediastinum in asthma are discussed. We suggest that the diagnosis of asthma should be considered in the differential diagnosis of pneumomediastinum in adolescence.

  15. Intestinal acariasis in Anhui Province

    Institute of Scientific and Technical Information of China (English)

    Chao-Pin Li; Jian Wang

    2000-01-01

    The mites found in stored food and house comprise a large group of subclass Acari, belonging to the suborder Acardida of the order Acarifornes. They can be found in dust and vacuum samples from floors, furniture, mattresses, Chinese herbal medicine, dry fruit, grain, flour, sugar, and bedding. These mites are nidicolous and feed on organic debris, including sloughed human skin, fungi, spilled food, pollen, etc. These mites are particularly prevalent in Chinese herbal medicine, dry fruit, grain, flour, sugar, beds, though carpeted floors near beds or couches may also have large numbers. The most common species are Acarus siro, Tyrophagus putrescentiae , Dermatophagoides farinae , D . pteronyssinus, Glycyphagus domesticus, G. Ornatus, Carpoglyphus lactis and Tarsonemus granarius, etc. The viability of mites in storage is quite strong and they can invade and parasitize the intestines of humans[1 -15]. They can cause pulmonary acariasis[16-25] , urinary acariasis[26-33] and so on. The dejecta of mites is a quite strong allergen and can cause different allergic diseases[34-44]. Intestinal acariasis can be caused by some mites related to the way of diet intake and invading against intestinal mucosa, intestinal muscle[45-5a]. The first report of intestinal acariasis caused by these mites was made by Hinman et al (1934)[45]. From then on, all kinds of studies on the disease have been reported gradually. In order to make an epidemiological survey of intestinal acariasis the investigation of the disease was taken in some areas of Anhui Province from 1989 to 1996.

  16. Quantitation of major allergens in dust samples from urban populations collected in different seasons in two climatic areas of the Basque region (Spain).

    Science.gov (United States)

    Echechipía, S; Ventas, P; Audícana, M; Urrutia, I; Gastaminza, G; Polo, F; Fernández de Corres, L

    1995-06-01

    We present the results of allergen content evaluation in 80 dust samples from 31 homes of atopic patients from two climatic areas (humid and subhumid), collected in two seasons of the year (autumn and winter). Monoclonal antibody-based immunoassays were used to quantify Der p 1, Der f 1, Der 2, Lep d 1, and Fel d 1. The results were compared according to climate, season, and the type of sensitization (Pyroglyphidae mites, storage mites, or grass pollens). We underline the predominance of Dermatophagoides pteronyssinus (89% of samples) over D. farinae (16% of samples) in our environment. Der p 1 rates were higher in the humid area (Mann-Whitney P < 0.001), especially in the autumn (Wilcoxon P < 0.05). Lep d 1 was detected in 23% of samples and Lep d 1 levels were higher in the homes of patients sensitized to storage mites (Mann-Whitney P < 0.05), whereas this allergen was not detected in the homes of pollen-allergic patients. Fel d 1 was detected in nine of the 31 homes (16% of samples) although there was a cat in only one home.

  17. House dust mites and their allergens in Danish mattresses -- results from a population based study

    DEFF Research Database (Denmark)

    Sidenius, Kirsten E; Hallas, Thorkil E; Poulsen, Lars K.

    2002-01-01

    The purpose of this study was to identify the level of house dust mites (HDMs) and their allergens in mattresses, not selected on their owners atopic status, and to find associated factors. Dust was collected from 68 mattresses. The recruitment was population-based and conducted during the screen......The purpose of this study was to identify the level of house dust mites (HDMs) and their allergens in mattresses, not selected on their owners atopic status, and to find associated factors. Dust was collected from 68 mattresses. The recruitment was population-based and conducted during...... the screening phase of a HDM intervention study. The visited persons declared to have had a "cold" bedroom the previous winter. HDMs were counted and dust was analysed by ELISA for Der 1 (= Der f 1+ Der p 1+ Der m 1). Multiple regression analysis was carried out to find housing conditions associated with high...... HDM levels. Type of housing, mattress age and self-assessed winter bedroom-temperature explained 47% of Der 1. Median concentrations were 3.77 microg Der 1/g and 1 HDM/0.1 g dust. Both immunochemically and microscopically Dermatophagoides farinae was dominant; D. pteronyssinus less frequent...

  18. Comparisons of the allergenic mite prevalence in dwellings and certain outdoor environments of the Upper Silesia (southwest Poland).

    Science.gov (United States)

    Solarz, Krzysztof; Senczuk, Liliana; Maniurka, Hanna; Cichecka, Ewelina; Peszke, Magdalena

    2007-12-01

    The occurrence of allergenic mites (pyroglyphid house-dust mites, acarid and glycyphagid storage mites and others) in dwellings and in synanthropic outdoor sites in a densely populated urban area was investigated. A total of 335 house dust samples were collected from dwellings at 7 different locations in Upper Silesia (Poland), including 84 samples from dwellings of Katowice. Mites were found in 188 of the samples examined (56.1%). A total of 4410 mite specimens were isolated and identified, including 4212 members of the family Pyroglyphidae (95.51%). Among them, Dermatophagoides farinae was predominant (75.3%), followed by D. pteronyssinus (18.6%) and Euroglyphus maynei (1.5%). Moreover, litter soiled with communal wastes was sampled. All 86 samples contained mites. A total of 7547 mite specimens were isolated including 6050 mites known as allergenic (80.5%). Among the astigmatid mites two acarids were dominant: Tyrophagus silvester and T. longior (28.7% and 25.1% of all mites). It should be stressed that 6 of the acarid mite species or genera found in outdoor environments are typical domestic mites (T. putrescentiae), or they have been collected from house dust samples (T. longior, Tyrolichus casei, Thyreophagus entomophagus, Mycetoglyphus fungivorus and Rhizoglyphus robini). The litter of synanthropic sites can therefore form a substantial source of infestation.

  19. Hypersensitivity to Parietaria officinalis pollen in newcomers to the area with the plant.

    Science.gov (United States)

    Cvitanović, S; Marusić, M; Juricić, M; Vrdoljak, E; Petrovecki, M; Rozga, A; Stavljenić-Rukavina, A

    1993-11-01

    Hypersensitivity to Parietaria officinalis (wall pellitory) pollen and other environmental allergens was studied in pollinosis patients allergic to P. officinalis pollen who were born in areas without P. officinalis and later moved to the city of Split, where P. officinalis is responsible for some 65% of pollinosis cases. Highly significant positive correlations were found for both the intensity of skin test reaction and concentration of specific serum IgE with the length of residence in the area. In contrast, the respective data on subjects hypersensitive to P. officinalis pollen allergen, but born and living in the area of Split, revealed a tendency to negative correlation between age and intensity of hypersensitivity to P. officinalis. A number of patients from both groups were tested for presence of serum IgE antibodies specific for 14 common environmental allergens. Hypersensitivity to P. officinalis pollen was associated with hypersensitivity to olive, mugwort, and birch pollen in newcomers; hypersensitivity to birch and, to some extent, olive pollen was significantly more frequent in newcomers than in autochthonous patients who were allergic to P. officinalis pollen. Regardless of whether the patients were autochthons or newcomers to the area with P. officinalis, hypersensitivity to P. officinalis mostly excluded hypersensitivity to Dermatophagoides farinae and D. pteronyssinus, and vice versa.

  20. Evaluation of the acarofauna of the domiciliary ecosystem in Juiz de Fora, State of Minas Gerais, Brazil

    Directory of Open Access Journals (Sweden)

    Ezequiel Oscarina da Silva

    2001-01-01

    Full Text Available From August 1999 to January 2000, samples of house dust were collected from 160 domiciles in the city of Juiz de Fora, State of Minas Gerais, Brazil. In 36 of these domiciles kitchen samples were obtained. Prevalence rate was 77.5%, varying according to the geographical sector. There were found 2,278 specimens of mites, with 1,530 (67.2% in the adult stage and 748 (32.8% in immature forms. The main species found were Dermatophagoides pteronyssinus, D. farinae, Euroglyphus maynei, Blomia tropicalis and Tyrophagus putrescentiae. In a minor incidence we found Lepidoglyphus destructor, Suidasia pontificiae, Chortoglyphus arcuatus, Cheyletus malaccensis, C. fortis, Ker bakeri, Cheletonella vespertilionis, C. caucasica and others. C. vespertilionis and C. caucasica were identified for the first time in the domiciliary ecosystem and in Brazil. The abundance rate and the infestation intensity were analyzed. There was a varied correlation between climatic conditions and positive domiciles and number of mites. The difference between the number of positive domiciles in the urban area and in the expanding urban area was significant and so was the difference between samples from the domiciles compared to those from the kitchens.

  1. The prevalence of indoor allergens in the Baltimore area: house dust-mite and animal-dander antigens measured by immunochemical techniques.

    Science.gov (United States)

    Lind, P; Norman, P S; Newton, M; Løwenstein, H; Schwartz, B

    1987-10-01

    Mattress dust samples from 42 homes in Baltimore, Md., were analyzed by ELISA and rocket immunoelectrophoresis (RIE) for major excrement allergens (P1 [equal to Dp 42], Df 6, and Dm 6) of house dust mites, Dermatophagoides pteronyssinus, D. farinae, and D. microceras, by RIE for cat major epithelial allergen (cat Ag 1) and horse major epithelial antigen (horse Ag 11), and by counter-current immunoelectrophoresis (CCIE) for cat and dog antigens. Dp 42 was detected by ELISA in 24 samples (57%) and Df 6 in 39 samples (92%). All samples were negative to Dm 6. Recorded levels varied from 50 ng of allergen per gram of dust (detection limit) to 15,000 ng/gm. A combined content of Dp 42 and Df 6 greater than 700 ng/gm was associated with a positive reaction in RIE. Concentrations of Dp 42 and Df 6 did not correlate (rS = 0.21; p greater than 0.05). Cat and dog antigens were frequently detected by CCIE (77% and 63%, respectively). CCIE titer to cat correlated well with quantitation of cat Ag 1 by RIE (p less than 0.01). Horse antigen was detected in few homes (5%). None of the specific allergen determinations correlated with levels of human serum albumin determined by RIE.

  2. [Mites in mattress dust and relevant environmental factors in student dormitories in Shenzhen].

    Science.gov (United States)

    Wang, Bin; Wu, Jie; Liu, Zhi-gang; Ran, Pi-xin; Gao, Qiao; Luo, Chun-hui; Ai, Mei

    2009-02-28

    Three hundred and eight mattress dust samples were collected from college dormitories in Shenzhen with a mite prevalence of 88% (271/308). From the samples, 6163 mites were isolated and identified. Dermatophagoides farinae, D. pteronyssinus and Blomia tropicalis were three most abundant species, occupying 29.7%, 21.7% and 17.9%, respectively. It was found that sex of the students, mattress cover (bamboo mat or bed sheet), with or without air conditioner installation, and daily using of air conditioner (8 h) had no significant influence on the mite prevalence (P>0.05). However, logistic regression analysis revealed that the risk of mite sensitization in male student dormitory was significantly lower than that in female dormitory (OR=0.55, P=0.038), and the risk of using bed sheets was significantly higher than using bamboo mats (OR=2.13, P=0.040). Both mite prevalence and the risk of mite sensitization significantly decreased with higher floor of the dormitory building.

  3. Allergy to house dust mites and asthma.

    Science.gov (United States)

    Milián, Evelyn; Díaz, Ana María

    2004-03-01

    House dust mites have been shown to be important sources of indoor allergens associated with asthma and other allergic conditions. Asthma is a chronic respiratory disease that affects millions of people worldwide, and numerous scientific studies have shown that the prevalence of asthma is increasing. The most common dust mite species around the world include Dermatophagoides pteronyssinus (Dp), Dermatophagoides farinae (Df), Euroglyphus maynei (Em) and Blomia tropicalis (Bt). Over the past three decades, many important allergens from these species have been identified and characterized at the molecular level. The biological function of several house dust mite allergens has been elucidated, with many of them showing enzymatic activity. However, Bt allergens remain the least studied, even though this mite is very common in tropical and subtropical regions of the world, including Puerto Rico. Therefore, it is very important to include Bt in diagnostic and therapeutic strategies for house dust mite induced allergy and asthma, particularly in areas where Bt exposure and sensitization is high. Recombinant DNA technology, as well as other molecular biology and immunological techniques, have played a fundamental role in advances towards a better understanding of the biology of house dust mites and their role in allergic diseases. This kind of study also contributes to the understanding of the complex immunologic mechanisms involved in allergic reactions. The development of effective diagnostic and therapeutic approaches depends on the continuity of research of house dust mite allergens. The objectives of this review are to describe the most important aspects of house dust mite allergy and to acquaint the scientific community with the latest findings pertaining to house dust mite allergens, particularly those derived from Bt.

  4. Skin Test Reactivity to Indoor Allergens Correlates with Asthma Severity in Jeddah, Saudi Arabia

    Directory of Open Access Journals (Sweden)

    Koshak Emad A

    2006-03-01

    Full Text Available Abstract Background There is increased emphasis on the role of indoor allergens in asthma. Objective To examine the spectrum of skin test reactivity (sensitization to indoor allergens and its correlation with asthma severity in Jeddah, Saudi Arabia. Methods Asthmatic patients referred to the allergy clinic at King Abdulaziz University Hospital (KAUH in Jeddah were studied. Measures of clinical severity were adopted from national and international asthma guidelines. The degree of sensitization was assessed by the wheal size (positive ≥ 3 mm from standard skin-prick tests for the following common indoor inhalant allergens: house dust mites (Dermatophagoides pteronyssinus [Dp] and Dermatophagoides farinae [Df], cat, and cockroach. Results Skin test results from 113 of 151 (74.8% asthmatic patients were positive for one or more allergens. The patients' ages ranged between 9 and 63 years (mean, 30 ± 13 years, and females constituted 65.5%. The predominant asthma severity level was moderate persistent (55.8%, followed by mild persistent (33.6%. The prevalences of sensitization to indoor allergens were as follows: Dp, 87% (3-25 mm [mean, 7 mm]; Df, 84% (3-20 mm [mean, 7 mm]; cat, 44% (3-15 mm [mean, 6 mm]; and cockroach, 33% (3-12 mm [mean, 4 mm]. Higher asthma severity levels were significantly correlated with the number of allergens with positive sensitization (R = 0.3, p Dp [degrees of freedom {df} = 16, p Df [df = 17, p df = 10, p df = 8, p Conclusions Immunoglobulin E-mediated skin test reactivity to indoor allergens, particularly to house dust mites, was common in asthmatic patients from Jeddah at KAUH. Increased sensitization was associated with higher levels of asthma severity, which is compatible with the literature. This emphasizes the importance of identifying sensitization to relevant indoor allergens in the clinical evaluation of asthmatic persons.

  5. Allergy to dust mites may contribute to early onset and severity of alopecia areata.

    Science.gov (United States)

    Li, S F; Zhang, X T; Qi, S L; Ye, Y T; Cao, H; Yang, Y Q; McElwee, K J; Zhang, X

    2015-03-01

    A higher risk of allergic diseases such as rhinitis, asthma and atopic eczema (atopic dermatitis) has been reported for patients with alopecia areata (AA) compared with the general population, but the significance of this is still largely unclear. To determine whether serum total or specific IgE play a role in the onset and severity of AA. We tested 461 serum samples from 351 patients with AA and 110 healthy controls (HC) for total IgE (tIgE) and specific IgE (sIgE) by ImmunoCAP-100 or in vitro test (IVT). The absolute value of tIgE was higher in patients with AA than in normal controls (P 120 IU/mL) detected in patients with AA (29.3%) was similar to that of HC (21.8%). Prevalences of raised sIgE against various allergens detected by ImmunoCAP-100 showed that Dermatophagoides pteronyssinus (Der p; 31.1%) and Dermatophagoides farinae (Der f; 29.0%) were the most common allergens. Similar results were found by IVT, with the most common response being against Der p/Der f (29.0%). However, the prevalences of tIgE and sIgE against dust mites (Der p and Der f) in patients with early-onset AA and severe AA were significantly higher than those with late-onset AA and mild AA (P = 0.02, P = 0.02 vs. P = 0.03 and P = 0.001, respectively). Notably, the increases in tIgE and sIgE were independent of atopy history. Allergy to dust mites may have an effect on the immune response in AA, and may contribute to its early onset and severity in patients of Chinese origin. © 2014 British Association of Dermatologists.

  6. Measurement of serum immunoglobulin E (IgE) specific for house dust mite antigens in normal cats and cats with allergic skin disease.

    Science.gov (United States)

    Taglinger, K; Helps, C R; Day, M J; Foster, A P

    2005-05-01

    The purpose of this study was to determine whether cats with allergic skin disease have significant concentrations of serum Immunoglobulin E (IgE) specific for antigens derived from the house dust mites (HDM) Dermatophagoides farinae (DF) and Dermatophagoides pteronyssinus (DP). Enzyme-linked immunosorbent assays (ELISA) were developed for this purpose. Binding of serum allergen-specific IgE was detected via the use of biotinylated Fc-epsilon receptor alpha chain protein (FcvarepsilonRIalpha). Following optimisation of the assay, serum samples from 59 cats with allergic skin disease and 54 clinically normal cats were screened. Results were expressed as ELISA units per ml (EU/ml) compared to a standard curve. Serological findings were correlated with the clinical presentation of affected cats. Cats with symptoms of feline allergic skin disease were grouped as follows: self-induced alopecia without lesions (group 1), papulocrusting dermatitis (group 2), eosinophilic granuloma complex (group 3), papular/ulcerative dermatitis of head and neck/facial dermatitis (group 4), and a combination of symptoms (group 5). Control normal cats comprised the final group (group 6). The Kruskal-Wallis test was used for statistical analysis. There was no significant difference between groups for DF- and DP-specific IgE concentrations with a p-value of 0.875 and 0.705, respectively. Although the FcvarepsilonRIalpha-based ELISA was able to detect house dust mite-specific feline IgE, the presence of this allergen-specific IgE correlates poorly with the presence of clinical manifestations of allergic skin disease. The results of this study question the clinical relevance of house dust mite-specific IgE in feline allergic skin disease.

  7. [Prevalence of sensitization to aeroallergens in 10 030 patients with allergic rhinitis].

    Science.gov (United States)

    Yang, You; Zhao, Yan; Wang, Cheng-shuo; Wang, Xiang-dong; Zhang, Luo

    2011-11-01

    To investigate the main inhaled allergens in patients with allergic rhinitis. Forty-three standardization allergens were used to perform skin prick test in 54 813 patients with chronic rhinitis. According to different gender and age groups, 10 030 patients with allergic rhinitis were divided into eight groups. The distributions of different allergens in the eight groups were analyzed. (1) The most common four allergens in male patients with allergic rhinitis were Dermatophagoides farina (Der f), Dermatophagoides pteronyssinus (Der p), Mugwort and Blaterlia Germanica. Der f, Der p, Mugwort and Chenopodium album were the most common in female patients. (2) In all groups, patients with dual positive allergens were the most common, followed by triple positive allergens and single positive allergen. (3) In single positive allergen group, the top four allergen in male 3-17 year group were Der f, Der p, Alternaria tenuis and Mugwort. In other male groups, the most common positive allergen were Der f, Der p, Mugwort and Blaterlia Germanica. Meanwhile, the top four positive allergens in female 3-17 year group were Der f, Der p, Mugwort and Alternaria tenuis, in 18-39 year group were Der f, Der P, Mugwort and Dandelion, in 40-59 year group were Der f, Der p Mugwort and mixed tree 1, in ≥60 year group were Derf, Der P, mixed animal hair and Mugwort. (4) In dual positive allergens group, the most common allergen combination was Der f+Der p, followed by Alternaria tenuis in patient of 3-17 year group. (5) In triple positive allergens group, the most common allergen combination was Der f+Der p+Blaterlia Germanica, followed by Der f+Der p+mixed animal hair. Dust mites, flowers pollen, herbs, Alternaria tenuis and Blaterlia Germanica were the most common allergens in patients with allergic rhinitis. However, the frequencies of the allergens were different in different age groups.

  8. Indoor mite allergen levels, specific IgE prevalence and IgE cross-inhibition pattern among asthmatic children in Haikou,southern China

    Institute of Scientific and Technical Information of China (English)

    ZHENG Yi-wu; CHEN Shi; LAI Xu-xin; Birgitte Gjesing; ZHONG Nan-shan; Michael D Spangfort

    2012-01-01

    Background Haikou locates in tropical island with unique mite propagation.The aim of this stuy is to determine mite allergens levels in Haikou,and to investigate the prevalence of mite specific IgE-sensitization and IgE cross-reactivity between house dust mites.Methods Allergen and antigen concentrations against six mite species were tested by enzyme-linked immunosorbent assay (ELISA).Specific IgE concentrations and cross-inhibitions were measured with ADVIA Centaur(R).Results Allergen or antigen Dermatophagoides pteronyssinus (Der p 1 ),Blomia tropicalis (Blo t ) and Tyrophagus putrescentia (Tyr p) were detected in dust samples.Dermatophagoides farinae (Der f 1 ),Lepidoglyphus destructor (Lep d 2),and Acarus siro (Aca s ) were found in very few samples.Specific IgE tests showed high prevalence of sensitizations against all tested mites with high IgE levels to Der p,Der f,and Blo t.Storage mites,Blo t,Tyr p,Lep d,and Aca s,could inhibit Der p from 0 to 50%.Storage mites could inhibit Der f between 30% and 100%.Der p IgE could be inhibited by Der f with up to 90%,and vice versa.Der p could inhibit Blot from 40% to 80%.Blot was able to fully inhibit IgE binding to Lep d,Tyr p,and Aca s compared to partial inhibition by Der p.Conclusions Der p is the dominating mite and has the highest specific IgE prevalence among asthmatic children.Blo t represents an important source of storage mite sensitization and some patients may be independently sensitized to both Der p and Blo t.High prevalence of sensitization to Der f may be due to IgE-mediated cross-reactivity with Der p and Blo t.

  9. Evaluation of serum specific IgE for the diagnosis of allergic rhinitis with multi-allergens

    Institute of Scientific and Technical Information of China (English)

    LIU Cheng-yao; ZHANG Yuan; HAN De-min; ZHANG Luo

    2010-01-01

    Background Objective evaluation of allergic rhinitis (AR) requires in vivo and in vitro tests. In vitro tests are important to assist or ensure the main allergens in multi-allergen-sensitive patients. The aim of this study was to evaluate the utility of serum specific IgE (slgE) in the diagnosis of AR patients with multi-allergens in the Chinese population.Methods Combining a positive skin prick test (SPT) and clinical history as the diagnostic reference criteria of AR, we estimated concentrations of slgE produced in response to the 7 most frequent allergens among 85 AR patients, using the UniCAP assay system.Results Among 85 individuals with positive SPT results and allergen-specific nasal symptoms, slgE concentration correlated well with SPT classes among all the tested allergens. Based on a clinical diagnosis and SPT results using a positive cut-off value of a class 1 score, the CAP test performed well and the sensitivity for different allergens ranged from 0.5 (giant ragweed) to 0.91 (Dermatophagoides pteronyssinus (Der p) and Dermatophagoides farinae (Der f), while specificity ranged from 0.93 (Der f) to 1.0 (animal hair, Derp and mugwort). When the cut-off score was adjusted to class 2, the sensitivity showed an increase overall while the remaining assessed items, including specificity, positive predictive value, negative predictive value and efficiency, showed an unacceptable decline.Conclusions Well-established serum slgE tests correlated well with SPTs. Setting a class 1 cut-off for positivity of SPT results was better than a class 2 setting for assessing the AR diagnostic value.

  10. Allergen micro-array detection of specific IgE-reactivity in Chinese allergy patients

    Institute of Scientific and Technical Information of China (English)

    ZHENG Yi-wu; ZHONG Nan-shan; Michael D Spangfort; LI Jing; LAI Xu-xin; ZHAO De-yu; LIU Xiao-fan; LIN Xiao-ping; Birgitte Gjesing; Paola Palazzo; Adriano Mari

    2011-01-01

    Background Allergen micro-arrays are powerful tools for screening of serum IgE-reactivity.In this study allergen micro-arrays were used to identify dominating IgE-binding allergens and cross-reactivity patterns among selected Chinese allergy patients.Methods The study was conducted using patient sera from the cities of Guangzhou,Nanjing,Chengdu and Shenyang.In total 100 sera with Dermatophagoides pteronyssinus (Der p) specific IgE-levels higher than 50 kU/L were selected for testing against 103 individual allergens.Results Among 100 selected patients, 95% showed IgE-reactivity towards house-dust mite allergens Dermatophagoides farinae (Der f) 1,Der f 2 and Der p 2 and 94% were IgE positive against Der p 1,and 60% of sera contained IgE reacting against allergen Euroglyphus maynei (Eur m) 2.IgE against cat allergen,Felisdomesticus (Fel d)1,was seen in 20%.Only 2% showed specific IgE-reactivity to Der p 10,a panallergen belonging to the tropomyosin family.Serum IgE-reactivity towards other allergens was in general low.IgE-reactivity against pollen allergens showed geographic differences.Conclusions This study clearly confirms that group 1 and group 2 are major allergens of house dust mites.These selected house-dust mite allergy patients are close to being mono-sensitized.Der p 10 is not an important allergen for cross-reactivity.Specific IgE-sensitization towards pollen allergens is low in southern China compared to other regions.The prevalence of food and stinging insect allergens known to give rise to IgE-mediated cross-reactivity is 2% or less.

  11. Characterization and evaluation of a Sarcoptes scabiei allergen as a candidate vaccine

    Directory of Open Access Journals (Sweden)

    Zhang Runhui

    2012-08-01

    Full Text Available Abstract Background Sarcoptic mange caused by the mite Sarcoptes scabiei is a worldwide disease affecting both humans and animals. Here we report the molecular characterization and evaluation of a recombinant S. scabiei tropomyosin (SsTm protein in a vaccination trial in rabbits. Methods The full-length cDNA was cloned in a bacterial pET vector, and the recombinant protein was expressed in BL21 (DE3 cells and purified. Using specific rabbit antiserum, tropomyosin was localized immunohistochemically in mite tissue sections. Vaccination trials with the recombiant SsTm was carried out in New Zealand rabbits. Results The full-length open reading frame (ORF of the 852 bp cloned gene from S. scabiei encodes a 32.9 kDa protein. The amino acid sequence showed 98.94%, 97.89% and 98.59% homology to Dermatophagoides farina and Dermatophagoides pteronyssinus group 10 allergens and Psoroptes ovis tropomyosin, respectively. Tropomyosin was localized immunohistochemically in mite tissue sections mainly in the mouthparts, legs and integument of the epidermis. The predicted cross-reactivity of SsTm indicated that it is an allergenic protein. While vaccination with the recombiant SsTm resulted in high levels of specific IgG (P S. scabiei challenge were observed. After challenge, specific IgG levels remained significantly higher than the control (P P > 0.05. However, the lesion areas in the vaccination group decreased at the end of the experiment compared with controls. Conclusions Although vaccination with recombinant SsTm did not efficiently control sarcoptic mange in rabbits, the immunogenic properties of tropomyosin suggest it may be developed as a vaccine with alternative adjuvants or delivery methods.

  12. Structural Analysis of Der p 1-Antibody Complexes and Comparison with Complexes of Proteins or Peptides with Monoclonal Antibodies

    Energy Technology Data Exchange (ETDEWEB)

    Osinski, Tomasz; Pomés, Anna; Majorek, Karolina A.; Glesner, Jill; Offermann, Lesa R.; Vailes, Lisa D.; Chapman, Martin D.; Minor, Wladek; Chruszcz, Maksymilian [INDOOR; (UV); (SC)

    2017-08-23

    Der p 1 is a major allergen from the house dust mite, Dermatophagoides pteronyssinus, that belongs to the papain-like cysteine protease family. To investigate the antigenic determinants of Der p 1, we determined two crystal structures of Der p 1 in complex with the Fab fragments of mAbs 5H8 or 10B9. Epitopes for these two Der p 1–specific Abs are located in different, nonoverlapping parts of the Der p 1 molecule. Nevertheless, surface area and identity of the amino acid residues involved in hydrogen bonds between allergen and Ab are similar. The epitope for mAb 10B9 only showed a partial overlap with the previously reported epitope for mAb 4C1, a cross-reactive mAb that binds Der p 1 and its homolog Der f 1 from Dermatophagoides farinae. Upon binding to Der p 1, the Fab fragment of mAb 10B9 was found to form a very rare α helix in its third CDR of the H chain. To provide an overview of the surface properties of the interfaces formed by the complexes of Der p 1–10B9 and Der p 1–5H8, along with the complexes of 4C1 with Der p 1 and Der f 1, a broad analysis of the surfaces and hydrogen bonds of all complexes of Fab–protein or Fab–peptide was performed. This work provides detailed insight into the cross-reactive and specific allergen–Ab interactions in group 1 mite allergens. The surface data of Fab–protein and Fab–peptide interfaces can be used in the design of conformational epitopes with reduced Ab binding for immunotherapy.

  13. Association study on ADAM33 polymorphisms in mite-sensitized persistent allergic rhinitis in a Chinese population.

    Directory of Open Access Journals (Sweden)

    Ruo-Xi Chen

    Full Text Available BACKGROUND: The ADAM33 gene has been identified as a potentially important asthma candidate gene and polymorphisms in this gene have been shown to be associated with asthma and seasonal allergic rhinitis. OBJECTIVE: To assess whether the ADAM33 polymorphisms are associated with persistent allergic rhinitis (PER due to house dust mites in a Chinese population. METHODS: In a hospital-based case-control study of 515 patients with mite-sensitized PER and 495 healthy controls, we genotyped seven single nucleotide polymorphisms (SNPs in ADAM33. Serum levels of eosinophil cationic protein, total IgE and allergen-specific IgE against Dermatophagoides pteronyssinus and Dermatophagoides farinae were measured by the ImmunoCAP assays. RESULTS: In the single-locus analysis, three polymorphisms, rs3918392 (F1, rs528557 (S2 and rs2787093, were significantly associated with mite-sensitized PER. SNP S2 was associated with significantly increased risk both of asthmatic and nonasthmatic mite-sensitized PER. In the combined genotypes analysis, individuals with 2-4 risk alleles had a significantly higher risk of mite-sensitized PER (adjusted OR = 1.99, 95% CI = 1.50-2.62 than those with 0-1 risk alleles. Haplotype-based association analysis revealed that the ACAGCCT haplotype might have potential to protect against mite-sensitized PER (adjusted OR = 0.67; 95% CI = 0.49-0.90. CONCLUSIONS: Polymorphisms in the ADAM33 gene may contribute to susceptibility of mite-sensitized PER in this Chinese population.

  14. The Effect of Indoor Environmental Characteristics on the Detection of House Dust Mite Der p2 and Der f2 in Asthmatics

    Directory of Open Access Journals (Sweden)

    Nimet Demirtaş

    2016-12-01

    Full Text Available Objective: Mites in house dust play a prominent role in the development of allergic sensitization and as a triggering factor that impairs disease control in asthma. The aim of the study was to determine whether the concentration of house dust mite allergens is associated with indoor conditions in stable asthmatics. Materials and Methods: During the study period, a total of 97 asthmatic patients were queried with a standard survey for their demographical characteristics and living environment. House dust samples from their houses were collected to quantitatively measure Dermatophagoides farinae (Der f2 and Dermatophagoides pteronyssinus (Der p2 levels by using the Enzyme-linked immunosorbent assay (ELISA method. Results: Using the quantitative ELISA method, measurable levels of mite allergens were found in 54.1% of the houses. Higher antigen detection rate was found in houses with visible mould and in those with moisture. The number of household was found to be significantly higher in houses with antigens than in those without antigens. When the indoor characteristics were evaluated by logistic regression analysis, larger number of household (≥4 was found to be a significant risk factor for the presence of mite allergens. The odds ratio for detecting Der p2 and Der f2 antigen was found to be 5.29 (confidence interval 2.18-12.86 (p<0.001. Conclusion: Mite allergen was detected in the house dusts of more than half of the cases by using quantitative ELISA method. Our results did not found any association between concentrations of allergens and indoor characteristics.

  15. Preparation of a polyclonal antibody that recognizes a unique galactoseβ1-4fucose disaccharide epitope.

    Science.gov (United States)

    Takeuchi, Tomoharu; Nishiyama, Kazusa; Saito, Saori; Tamura, Mayumi; Fuwa, Takashi J; Nishihara, Shoko; Takahashi, Hideyo; Natsugari, Hideaki; Arata, Yoichiro; Kasai, Ken-ichi

    2015-08-14

    Galactoseβ1-4fucose (Galβ1-4Fuc) is a unique disaccharide unit that has been found only in the N-glycans of protostomia. We demonstrated that this unit has a role as an endogenous ligand for Caenorhabditis elegans galectins. This unit is also recognized by fungal and mammalian galectins possibly as a non-self glycomarker. In order to clarify its biological function, we made a polyclonal antibody using (Galβ1-4Fuc)n-BSA as the antigen, which was prepared by crosslinking Galβ1-4Fuc-O-(CH2)2-SH and BSA. The binding specificity of the antibody was analyzed by frontal affinity chromatography, and it was confirmed that it recognizes naturally occurring N-glycans containing the Galβ1-4Fuc unit linked to the reducing-end GlcNAc via α1-6 linkage. By western blotting analysis, the antibody was also found to bind to (Galβ1-4Fuc)n-BSA but not to BSA or asialofetuin, which has N-glycan chains containing Galβ1-4GlcNAc. Western blotting experiments also revealed presence of stained proteins in crude extracts of C. elegans, the parasitic nematode Ascaris suum, and the allergenic mite Dermatophagoides pteronyssinus, while those from Drosophila melanogaster, Mus musculus, and the allergenic mites Dermatophagoides farinae and Tyrophagus putrescentiae were negative. This antibody should be a very useful tool for research on the distribution of the Galβ1-4Fuc disaccharide unit in glycans in a wide range of organisms.

  16. Alteraciones patológicas producidas por la inoculación subcutánea de los extractos alergénicos de Blomia tropicalis y Dermatophagoides siboney en ratas y ratones

    Directory of Open Access Journals (Sweden)

    D. Fuentes

    2003-01-01

    Full Text Available Los extractos alergénicos se emplean en tratamientos de inmunoterapia, ya que son capaces de inducir cambios inmunológicos en la respuesta alérgica, reduciendo los síntomas clínicos de la enfermedad. Estas preparaciones terapéuticas se aplican directamente al hombre, por lo que nos propusimos como objetivo de este trabajo evaluar los cambios patológicos producidos por la administración subcutánea durante 28 días del extracto alergénico de Dermatophagoides siboney y Blomia tropicalis en ratas y ratones. Se encontraron lesiones circunscritas en la hipodermis del lugar de aplicación y caracterizadas por abundantes células redondas: linfocitos, células plasmáticas y macrófagos, agrupadas fundamentalmente alrededor de los vasos sanguíneos y terminaciones nerviosas, así como algunos leucocitos polimorfonucleares de tipo neutrófilo y células cebadas. Se concluye que la administración repetida de los extractos alergénicos de Dermatophagoides siboney y Blomia tropicalis en ratas y ratones no provoca alteraciones patológicas

  17. Dicty_cDB: Contig-U01123-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 46 2.9 1 ( CB284403 ) DF1148 Dermatophagoides farinae cDNA library Derm... 46 2.9 1 ( GO192862 ) CAGB13789.fwd CAGB Alvin...ella pompejana Normalized... 46 2.9 1 ( GO192861 ) CAGB13789.rev CAGB Alvinella pompejana Norm

  18. Mite hypersensitivity in patients with rhinitis and rhinosinusitis living in a tropical environment.

    Science.gov (United States)

    Sánchez-Borges, M; Fernández-Caldas, E; Capriles-Hulett, A; Caballero-Fonseca, F

    2014-01-01

    Rhinitis and rhinosinusitis are major concerns in public health. Mites are important aetiological agents in the tropics. The present study investigated the in vivo response to mite allergens in patients with rhinitis and rhinosinusitis. All patients with presumptive nasal allergy were included. Skin tests were done with inhalants and mite extracts. Patients were classified as allergic or non-allergic according to skin tests and history. Out of 229 patients, 175 (76.4%) showed positive skin tests. Allergic patients showed positivity to mites in 97.1% of cases, 51.4% to dog dander; 40.5% to cat dander; 36.5% to German cockroach; 22.8% to moulds; and 21.1% to grass pollens. Dermatophagoides farinae induced responses in 90.8% of patients, D. pteronyssinus in 90.1%, Blomia tropicalis in 74.8%, Glycyphagus domesticus in 62.2%, Chortoglyphus arcuatus in 58.2%, Acarus siro in 46.2%, Lepidoglyphus destructor in 35.4%, and Tyrophagus putrescentiae in 35.0%. Higher correlations were found between skin test diameters induced by mites from the same family. Sensitisation to inhalant allergens is present in 76% of allergy clinics' patients with rhinitis or rhinosinusitis. Our results confirm previous observations showing that mites constitute the most important cause of respiratory allergy in tropical settings and suggest that mite allergen cross-reactivity is responsible for the positivity of skin tests to mites not present in the patient's environment since the species Glycyphagus, Chortoglyphus, Acarus, Lepidoglyphus and Tyrophagus have not been found in Caracas house dust. Copyright © 2012 SEICAP. Published by Elsevier Espana. All rights reserved.

  19. Sensitisation to mites in a group of patients with asthma in Yaounde, Cameroon: a cross-sectional study.

    Science.gov (United States)

    Pefura-Yone, Eric Walter; Kengne, André Pascal; Kuaban, Christopher

    2014-01-03

    Sensitisation of asthmatic patients to mites in sub-Saharan Africa has been less described. The aim of this study was to assess the prevalence and determinants of sensitisation to mites in asthmatic adolescents and adults in Yaounde, Cameroon. This was a cross-sectional study. Logistic regression models were employed to investigate the determinants of sensitisation to mites. This study was carried out at the Jamot Hospital and CEDIMER private centre, in Yaounde, capital city of Cameroon. All asthmatic patients received in consultations from January 2012 to June 2013 and in whom prick-skin tests for perennial aeroallergens were performed were included. Prevalence of sensitisation to mites and associated factors. In total, 201 patients (132 being women, 65.7%), with a median age of 36 (25th-75th centiles: 20-54) years were included, with 135 (67.2%) having a positive skin test for mites. Sensitisation to Dermatophagoïdes pteronyssinus, Dermatophagoides farinae and Blomia tropicalis was found in 53.2%, 49.8% and 47.8% of the patients, respectively. Intermittent rhinitis (16.3% vs 7.6%) and persistent rhinitis (43.0% vs 22.7%) were more frequent in sensitised patients than in the non-sensitised ones (pmites were sensitisation to Alternaria alternata (adjusted OR 14.98 (95% CIs 1.96 to 114.4)) and sensitisation to Blattella germanica (3.48 (1.34 to 9.00)). Sensitisation to mites was found in about two-thirds of asthmatic patients in this setting, with a frequent multiple sensitisations to A alternata and Blattella germanica. Systematically investigating asthmatic patients for mites' sensitisation and determinants will help optimising the care in this setting by combining the aetiological treatment for the allergy with symptomatic treatment for asthma, in order to modify the natural course of the disease.

  20. Analysis of allergens with allergic rhinitis in Rizhao%山东省日照地区变应性鼻炎患者过敏原分析

    Institute of Scientific and Technical Information of China (English)

    黄咏梅; 乔秀军; 尹华; 牟军伟

    2011-01-01

    Objective To find out common allergens in Rizhao by investigating the allergens in patients with allergic rhinitis.Method The allergens in 686 patients with allergic rhinitis in Rizhao were detected by skin prick test.Results 509 of 686(74.20%)cases presented positive reaction,in which,the positive rate was 70.99%in inhalation group and 30.61%in food group,the most common inhalation allergen was pteronyssinus,then was dermatophagoides farinae,cockroaches;The most common food allergen was the jellyfish,then was peaches,peanuts.Conclusion The inhaled allergens were the main risk factors for allergic rhinitis,in which,mites was the most common allergens.%目的 通过对山东省日照地区变应性鼻炎患者进行过敏原检测及分析,筛查该地区常见过敏原.方法 采用皮肤点刺法对686例变应性鼻炎患者进行过敏原检测.结果 686例患者中过敏原阳性509例(74.20%),其中吸入组阳性率70.99%,较高的为户尘螨、粉尘螨、蟑螂;食物组阳性率30.61%,较高的为海蜇、桃子、花生.结论 吸入性变应原为日照地区过敏性鼻炎主要的致病因素,螨类为最常见的变应原.

  1. Aeroallergen sensitivity among patients suffering from bronchial asthma in Bangalore

    Directory of Open Access Journals (Sweden)

    Giriyanna Gowda

    2013-01-01

    Full Text Available Background: Asthma is a serious public health problem throughout the world and its prevalence has increased in last 2-3 decades. Allergens are one of the many factors which trigger an attack of asthma. Skin prick test is useful in identifying the offending allergen in bronchial asthma. Aim: To identify the possible offending allergens in patients of bronchial asthma. Materials and Methods: The study was a descriptive study conducted at allergy center, Kempegowda Institute of Medical Sciences Hospital and Research Center, Bangalore from January to December 2011. Skin prick test was done in 139 patients suffering from bronchial asthma using 49 allergens extracts. Statistical Analysis: Frequency, proportions, Chi-square test, odds ratio, and 95% confidence interval was used. Results: Out of 139 patients who underwent the skin prick tests, 40% (56 were males and 60% (83 were females. Majority, that is, 60% were in the age group of 21-40 years. Forty-three percent (60 had family history of asthma/atopy, 80% (111 had allergic rhinitis, 24% (34 had chronic urticaria, and 24% (33 had allergic conjunctivitis. Out of 139 patients, 100 (71.94% were sensitive for one or more allergens. The common offending allergens found in the study were dust mites (Dermatophagoides farinae and D. pteronyssinus (DF and DP - 49.28%, dusts - 7.2%, pollens - 6.77%, insects - 6.62%, fungi - 4.53%, and epithelia - 1.92%. Conclusion: The most common allergens in bronchial asthma were dust mites followed by dusts and pollens. Identifying possible allergens in asthma patients help in allergen avoidance and immunotherapy in these patients.

  2. Effectiveness of Specific Sublingual Immunotherapy in Korean Patients with Atopic Dermatitis

    Science.gov (United States)

    You, Hyang-Suk; Yang, Min-Young; Kim, Gun-Wook; Cho, Hyun-Ho; Kim, Won-Jeong; Mun, Je-Ho; Song, Margaret; Kim, Hoon-Soo; Ko, Hyun-Chang; Kim, Moon-Bum

    2017-01-01

    Background Sublingual immunotherapy (SLIT) with house dust mites (HDM) preparation has recently been proven to be beneficial for treating allergic rhinitis and asthma. However, there has been no report regarding the efficacy and safety of SLIT in Korean patients with atopic dermatitis (AD). Objective We intended to investigate the efficacy and safety of SLIT in Korean patients with AD. Methods A total of 34 patients with AD and immunoglobulin E (IgE)-proven HDM sensitization (Class ≥3) were recruited. Eczema area and severity index (EASI) score, total serum IgE level, specific IgE assays to Dermatophagoides pteronyssinus, D. farinae, and adverse effects were recorded during follow-up. "Responder" was defined as a patient with ≥30% improvement in EASI score after SLIT. Results Twenty-three patients continued SLIT for 12 months or more, whereas 3 patients (8.8%) dropped out because of exacerbation of dermatitis, and 8 patients (23.5%) were lost to follow-up. The average duration of SLIT treatment was 22.4 months (range, 12~32 months). EASI scores reduced significantly after 6 months of treatment (p<0.05) compared with those at baseline. A total of 18 patients were determined to be responders to SLIT after 6 months. Total and specific IgE serum levels did not significantly reduce after SLIT. No patients experienced serious adverse events, with the exception of two patients who developed transient lip and tongue swelling. Conclusion Our study demonstrated that SLIT with HDM extracts is effective and tolerable in Korean patients with AD. Further controlled long-term trials are required to reinforce the current results. PMID:28223739

  3. Evaluation of impermeable covers for bedding in patients with allergic rhinitis.

    Science.gov (United States)

    Terreehorst, Ingrid; Hak, Eelko; Oosting, Albert J; Tempels-Pavlica, Zana; de Monchy, Jan G R; Bruijnzeel-Koomen, Carla A F M; Aalberse, Rob C; Gerth van Wijk, Roy

    2003-07-17

    Encasing bedding in impermeable covers reduces exposure to house-dust mites, but the clinical benefit of this intervention as part of mite-avoidance measures for patients with allergic rhinitis is not known. We performed a multicenter, randomized, placebo-controlled trial of one year of use of impermeable bedding covers in the bedrooms of patients with rhinitis who were sensitized to house-dust mites to determine the effects on the signs and symptoms of disease. Three participating university medical centers enrolled 279 patients with allergic rhinitis who were randomly assigned to receive impermeable or non-impermeable (control) covers for their mattress, pillow, and duvet or blanket. At the start of the study, all participants received information on general allergen-avoidance measures. The severity of rhinitis was measured on a rhinitis-specific visual-analogue scale and by means of a daily symptom score and nasal allergen provocation testing. We also measured the concentrations of Dermatophagoides pteronyssinus (Der p1) and D. farinae (Der f1) in dust from patients' mattresses, bedroom floors, and living-room floors at base line and after 12 months as a measure of the efficacy of the intervention. A total of 232 patients completed the study. There was a significant reduction in Der p1 and Der f1 concentrations in the mattresses of the impermeable-cover group, whereas there was no significant reduction in the control group. However, there was no significant effect on the clinical outcome measures. Analyses of subgroups defined according to age, level of exposure, type and severity of sensitization, or characteristics of the patient's home had similar results. Mite-proof bedding covers, as part of a structured allergy-control program, reduced the level of exposure to mite allergens. Despite the success of the intervention, this single avoidance measure did not lead to a significant improvement of clinical symptoms in patients with allergic rhinitis. Copyright

  4. 21 CFR 137.300 - Farina.

    Science.gov (United States)

    2010-04-01

    ..., other than durum wheat and red durum wheat, to such fineness that, when tested by the method prescribed... (8-inch) full-height frames, complying with the specifications for such cloth set forth in “Official...

  5. 21 CFR 137.305 - Enriched farina.

    Science.gov (United States)

    2010-04-01

    ... comply with the limit for moisture prescribed by § 137.300(a). (6) In determining whether the ash content...; such carrier is used only in the quantity necessary to effect an intimate and uniform admixture of such...

  6. Broncoprovocação com solução salina hipertônica em crianças asmáticas Brocoprovocación con solución salina hipertónica en niños asmáticos Bronchoprovocation with hypertonic saline solution in asthmatic children

    Directory of Open Access Journals (Sweden)

    Cínthia Maria X. Costa

    2012-09-01

    Full Text Available OBJETIVO: Verificar se o teste de broncoprovocação, com solução salina hipertônica a 4,5%, permite detectar o gradiente de resposta em crianças e adolescentes com asma, segundo a gravidade da enfermidade. MÉTODOS: Estudo transversal composto por 75 pacientes asmáticos com idades entre seis e 18 anos. Os pacientes foram classificados pela gravidade (intermitente associada à persistente leve - IL, e persistente moderada associada à grave - MG e segundo a presença de sensibilização a aeroalérgenos (testes cutâneos de hipersensibilidade imediata a Dermatophagoides pteronyssinus, D. farinae e Blomia tropicalis ou não (atópicos versus não atópicos. Todos foram submetidos ao teste de broncoprovocação com solução salina hipertônica a 4,5%, considerando-se o resultado positivo se havia redução do volume expiratório forçado no primeiro segundo (VEF1 ≥20%. RESULTADOS: 60 indivíduos eram atópicos. A frequência de positividade do teste de broncoprovocação foi maior no Grupo MG do que no IL (93 versus 65%. O tempo necessário para a queda de 20% do VEF1 para o grupo de atópicos foi menor no MG quando comparado ao IL: 90 (30 - 330 versus 210 (30 - 690 segundos, com pOBJETIVO: Verificar si la prueba de broncoprovocación, con solución salina hipertónica a 4,5%, permite detectar el gradiente de respuesta en niños y adolescentes con asma, según la gravedad de la enfermedad. MÉTODOS: Estudio transversal, compuesto por 75 pacientes asmáticos, con edades entre seis y 18 años. Los pacientes fueron clasificados por la gravedad (intermitente asociada a persistente liviana - IL - y persistente moderada asociada a grave - MG y según la presencia de sensibilización a aeroalérgenos (pruebas cutáneas de hipersensibilidad inmediata a Dermatophagoides pteronyssinus, D. farinae y Blomita tropicalis o no (atópicos versus no atópicos. Todos fueron sometidos a la prueba de broncoprovocación con solución salina hipertónica a 4

  7. Sensitization to Aspergillus species is associated with frequent exacerbations in severe asthma

    Directory of Open Access Journals (Sweden)

    Goh KJ

    2017-04-01

    Full Text Available Ken Junyang Goh,1 Anthony Chau Ang Yii,1,2 Therese Sophie Lapperre,1,2 Adrian KW Chan,1–3 Fook Tim Chew,4 Sanjay H Chotirmall,5,* Mariko Siyue Koh1–3,* 1Department of Respiratory and Critical Care Medicine, Singapore General Hospital, 2Duke-National University of Singapore Medical School, 3Allergy Centre, Singapore General Hospital, 4Department of Biological Sciences, Faculty of Science, National University of Singapore, 5Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore *These authors contributed equally to this work Background: Severe asthma is a largely heterogeneous disease with varying phenotypic profiles. The relationship between specific allergen sensitization and asthma severity, particularly in Asia, remains unclear. We aim to study the prevalence of specific allergen sensitization patterns and investigate their association with outcomes in a severe asthma cohort in an Asian setting. Methods: We conducted a cross-sectional study of patients receiving step 4 or 5 Global Initiative for Asthma treatment. Univariate and multivariate analyses were performed to assess the association between sensitization to a specific identifiable allergen by skin prick test (SPT and uncontrolled asthma (defined in our study as the use of ≥2 steroid bursts or hospitalization in the past year, a history of near-fatal asthma or evidence of airflow obstruction on spirometry. Results: Two hundred and six severe asthma patients (mean age 45±17 years, 99 [48.1%] male were evaluated. Of them, 78.2% had a positive SPT to one or more allergens. The most common allergen to which patients were sensitized was house dust mites (Blomia tropicalis, Dermatophagoides pteronyssinus and Dermatophagoides farinae. Also, 11.7% were sensitized to Aspergillus species. On multivariate analysis, Aspergillus sensitization was associated with uncontrolled asthma (odds ratio 6.07, 95% confidence interval 1.80–20.51. In particular, Aspergillus

  8. [Type I hypersensitivity to nocturnal Lepidoptera in a Costa Rican allergic population].

    Science.gov (United States)

    Sandí-Villalobos, Cindy; Jaikel-Víquez, Daniela; Riggioni-Cordero, Olman

    2015-01-01

    Antecedentes: en la consulta de Alergología se realiza un tamizaje rutinario con diversos alergenos, entre ellos: mohos, alimentos, cucarachas y ácaros, que logra diagnosticar a gran parte de los pacientes atendidos. Sin embargo, hay individuos con síntomas de alergia que resultan negativos en las pruebas por punción con los alergenos disponibles en el mercado. Las trampas de luz UV que se colocan en el Valle Inter-montano Central de Costa Rica para mosquitos muestran una gran cantidad de captura de lepidópteros nocturnos, lo que motivó a pensar en la importancia de la alergenicidad de este género de nivel de la región tropical húmeda. Objetivos: determinar el porcentaje de pacientes sensibilizados a los lepidópteros nocturnos y dar a conocer la importancia de estos insectos como alergenos ambientales. Material y método: análisis retrospectivo de los expedientes de la Sección de Alergología de la Clínica Médica Herediana, Heredia, Costa Rica, de febrero de 2011 a febrero de 2012 para determinar el porcentaje de pacientes alérgicos a los lepidópteros nocturnos, a los ácaros de polvo doméstico (Blomia tropicalis, Dermatophagoides pteronyssinus, Dermatophagoides farinae y Tyrophagus putrescentiae) y a insectos (Periplaneta americana y Musca domestica). A su vez, se analizaron los aspectos demográficos y los síntomas de los pacientes. Resultados: se incluyeron 347 pacientes (217 mujeres y 130 hombres, 36.32 ± 18.10 años). El 63% de los pacientes eran alérgicos a los ácaros del polvo doméstico, 39% a los lepidópteros nocturnos, 28% a Periplaneta americana y 24% a Musca domestica. El 2% de los pacientes alérgicos a sólo un alergeno estaba monosensibilizado contra los alergenos de los lepidópteros nocturnos. Conclusión: se recomiendan las pruebas continuas de alergia a lepidópteros nocturnos, porque son los insectos a los que la mayoría de los pacientes estudiados tuvo alergia.

  9. [Profile of sensitization to allergens in children with atopic dermatitis assisting to Allergology Service of University Hospital, Nuevo Leon, Mexico].

    Science.gov (United States)

    Yong-Rodríguez, Adrián; Macías-Weinmann, Alejandra; Palma-Gómez, Samuel; Arias-Cruz, Alfredo; Pérez-Vanzzini, Rafael; Gutiérrez-Mujica, José Julio; González-Díaz, Sandra Nora

    2015-01-01

    Antecedentes: la sensibilización a alergenos observada en la dermatitis atópica aumenta el riesgo del niño a padecer rinitis alérgica y asma. Los estudios recientes indican que entre mayor actividad de proteasas haya en los alergenos a los que se está sensibilizado, hay mayor defecto en la barrera cutánea y mayor gravedad de la enfermedad. Objetivos: conocer el patrón de sensibilización a los alergenos en niños con dermatitis atópica atendidos en el Servicio de Alergología del Hospital Universitario de la Universidad Autónoma de Nuevo León y conocer si estos niños tienen mayor sensibilización a los antígenos con actividad proteolítica. Material y método: estudio retrospectivo en el que revisamos los reportes de las pruebas cutáneas por punción realizadas en nuestro servicio a niños de 5 meses a 16 años de edad, con diagnóstico de dermatitis atópica, de enero de 2012 a enero de 2014. Evaluamos la frecuencia de sensibilización a aeroalergenos y alimentos, así como el tamaño de la roncha en la respuesta cutánea para cada alergeno en particular. Resultados: se incluyeron los reportes de pruebas cutáneas de 66 niños, 30 hombres y 36 mujeres. Cuarenta y seis pacientes estaban sensibilizados a aeroalergenos y 38 a alimentos. Los ácaros del polvo de casa (Dermatophagoides pteronyssinus/Dermatophagoides farinae) fueron los alergenos con mayor frecuencia de respuesta positiva en las pruebas cutáneas. De los niños con sensibilización a alimentos, sólo los niños sensibilizados a la leche de vaca, al huevo y al pescado tuvieron una roncha mayor de 6 mm de diámetro. CONCLUSIÓN: en los niños con dermatitis atópica es común la sensibilización a aeroalergenos con alta actividad de proteasas y la polisensibilización es muy común. La sensibilización a alimentos es común en estos pacientes, pero sólo un pequeño porcentaje de ellos muestra respuestas cutáneas lo suficientemente grandes para relacionarlas con gravedad de la enfermedad.

  10. 11 Efficacy and Tolerability of HDM Injective Immunotherapy With Monomeric Allergoid

    Science.gov (United States)

    Compalati, Enrico; Atzeni, Isabella; Cabras, Sergio; Fancello, Paolo; Gaspardini, Giulio; Longo, Rocco; Patella, Vincenzo; Tore, Giorgio

    2012-01-01

    Background Subcutaneous immunotherapy (SCIT) is an effective treatment of respiratory allergy and carbamylated monomeric allergoids (monoids), by virtue of their reduced IgE-binding activity, resulted clinically safe by sublingual administration. Purpose of this study was to investigate the efficacy and tolerability of immunotherapy with house dust mites (HDM) monoid administered by injective route in patients with allergic rhinoconjunctivitis (AR). Methods A preparation of 0.70 mL of 10 BU/mL containing modified extract with 50% Dermatophagoides pteronyssinus and 50% Dermatophagoides farinae (amount of major allergen: 4 μg of group 1 per milliliter) was delivered monthly for 12 months, following a 5-week build-up induction phase (0.10–0.20–0.30–0.50–0.70 mL), to 58 patients (60% males, mean age 25.1 ± 12.7) suffering from AR due to mites for at least 2 years, whereas 60 patients with similar baseline characteristics were observed as controls. All patients were allowed to assume traditional drug therapy for their condition. At the end of the study changes from baseline in symptoms scores, in number of days with drug assumption, in severity of AR (according to ARIA classification) were compared between the 2 groups; moreover an overall assessment of clinical efficacy and tolerability was based on patients' and physicians' judgements (unsatisfactory, mild, good, optimal). Results In respect to baseline both groups showed, after 1 year, an improvement in symptoms score (P < 0.001) with a significant difference in favour of SCIT group (P < 0.05). Days of drug intake were significantly lower in patients receiving SCIT (P < 0.05). The number of patients with severe AR decreased in the first group while no variation was observed in controls. The subjective clinical overall assessment was optimal in 31 cases and good in 24 according to physicians' and patients' judgements; similarly 38 patients judged tolerability as optimal and 18 as good, whereas according to

  11. Dose-Dependent Immunological Responses after a 6-Month Course of Sublingual House Dust Mite Immunotherapy in Patients with Allergic Rhinitis.

    Science.gov (United States)

    Didier, Alain; Campo, Paloma; Moreno, Francisco; Durand-Perdriel, François; Marin, Alicia; Chartier, Antoine

    2015-01-01

    House dust mite (HDM) immunotherapy has proven efficacy in treating allergic rhinitis (AR) symptoms. This trial evaluated the dose-response relationship of SLIToneULTRA® HDM mix based on immunological parameters and safety in subjects with moderate-to-severe HDM AR not controlled by symptomatic medication. A randomized, parallel-group, open-label, clinical trial compared 50/150/300 standard reactivity unit (SRU) doses of SLIToneULTRA® HDM mix for 6 months. Subjects had moderate-to-severe HDM AR, positive skin prick and IgE against Dermatophagoides pteronyssinus/Dermatophagoides farinae (DP/DF). The primary end point was change from baseline in the IgE-blocking factor against DP after 6 months. Secondary end points measured changes in the IgE-blocking factor for DP at 3 months and for DF at 3 and 6 months, and in IgG4 and specific IgE to DP/DF after 3 and 6 months. Tolerability was assessed through the evaluation of all adverse events (AEs). A total of 219 subjects were randomized and 196 completed the trial. Dose effect was significant on DP IgE-BF after 6 months (p = 0.018). The change in the DP IgE-blocking factor at a 300-SRU dose was higher than at other doses after 3 (p = 0.008) and 6 (p = 0005) months of treatment. Similar changes were observed for IgG4 and allergen-specific IgE. The number of AEs increased with the dose and were mild-to-moderate, with no severe treatment-related AEs reported. The most frequent AEs were oral/tongue pruritus, mouth oedema and abdominal upper pain. Data showed a dose-response for immunological markers and safety with a better immunological response for 300 SRU. The highest dose (300 SRU daily) was considered as the optimal maintenance dose. © 2016 The Author(s) Published by S. Karger AG, Basel.

  12. Aeroallergen spectrum of patients with child allergic rhinitis in Changsha area of China%长沙地区562例变应性鼻炎患儿变应原谱分析

    Institute of Scientific and Technical Information of China (English)

    钟竹青; 王芳; 王天生; 李亮明; 谭国林

    2011-01-01

    目的:调查长沙地区变应性鼻炎患儿的变应原分布情况及其阳性率,并分析其相关因素.方法:应用13种标准化吸入变应原对562例变应性鼻炎患儿进行变应原皮肤点刺试验,并详细采集病史资料.结果:457例呈阳性反应(81.3%),其主要变应原均为粉尘螨和屋尘螨,其次为热带螨、美洲大蠊、德国小蠊及狗毛等.花粉变应原在该组十分少见,城市儿童变应性鼻炎的患病率显著高于农村(P<0.01).结论:长沙地区变应性鼻炎患儿的主要变应原为尘螨,与居住环境有关,多数患儿可应用螨变应原进行特异性免疫治疗.变应原皮肤点刺试验应用于儿童变应性鼻炎的诊断是一种安全的方法.%Objective:To investigate aeroallergen spectrum and allergy positive rates of patients with child allergic rhinitis and analyze its related factors. Method:Skin prick test was carried out in 562 cases with child allergic rhinitis using 13 inhaled allergens, and detailed history was collected in all cases. Result: Four hundred and fifty-seven (81. 3%) of 562 cases showed positive reaction to at least one allergen out of 13 allergens. The most common allergens found in patients was dermatophagoides farinae and dermatophagoides pteronyssinus,and positive reaction was 93. 1% in child allergic rhinitis, followed by tropical mite, periplaneta americana, blattella germanica and dog hair. The pollen allergen, most common in American and European, was pretty rare in this study. The prevalence of child allergic rhinitis was significantly higher in urban than in rural(P<0. 01). There was no adverse effect appeared in children with allergic rhinitis underwent skin prick test. Conclusion: The major allergen of allergic rhinitis is mite for child allergic rhinitis, and relates to housing enviroment. Most of patients with child allergic rhinitis can be treated by the mite specific immunotherapy. The skin prick test is a safe technique for diagnosis of

  13. Aeroallergen spectrum of patients with allergic rhinitis in Enshi area%恩施州地区变应性鼻炎患者的吸入变应原谱分析

    Institute of Scientific and Technical Information of China (English)

    刘军; 周意; 万静; 刘忠

    2011-01-01

    目的:调查恩施州地区变应性鼻炎患者变应原的分布,并分析其相关因素.方法:应用13种标准化吸入变应原对463例疑似变应性鼻炎患者进行皮肤点刺实验,并详细采集病史资料.结果:387例(83.6%)患者变应原呈阳性反应,阳性率相对较高的变应原依次为粉尘螨、屋尘螨、热带螨、美洲大蠊、德国小蠊及狗毛.对单类变应原呈阳性反应的占51.9%,其中大多数是螨类,占49.6%.变应原阳性强弱与家族史、是否伴哮喘、年龄及居住环境显著相关.结论:恩施州地区变应性鼻炎的主要变应原为尘螨,其阳性强弱与遗传、哮喘、年龄及居住环境有关.%Objective: To investigate aeroallergen spectrum of patients with allergic rhinitis in Enshi area and analyze its related factors. Method:Skin prick test was carried out in 463 patients with allergic rhinitis with 13 inhaled allergens. Detailed history was collected in all cases. Result: Three hundred and eigty-seven cases(83. 6 %)showed positive reaction to at least one allergen of 13 allergens. The most common allergens found in patients were dermatophagoides farinae and dermatophagoides pteronyssinus, followed by tropical mite、periplaneta americana,blattella germanica and dog hair. The positive reaction rate of single specie allergen were 51.9%, and most of them was positive to mites(49. 6 %). The positive reaction degree was significantly related to family history, asthma ,age and housing enviroment. Conclusion:The major allergen of allergic rhinitis was mite. The positive reaction degree related to genetics, asthma, age and housing enviroment.

  14. 上海地区变应性鼻炎患儿主要变应原的临床研究%Variability analysis of allergic rhinitis related allergens in children

    Institute of Scientific and Technical Information of China (English)

    朱华明; 张玉君; 张维天; 苏开明; 时海波

    2011-01-01

    目的 了解近年来上海地区变应性鼻炎患儿的变应原特征,为儿童变应性鼻炎的防治提供临床依据.方法 2005年6月~2010年5月间,对上海地区拟诊变应性鼻炎的儿童行皮肤点刺试验(SPT),分析检测结果,并探讨了阳性病例在性别、年龄、检测的年份及季节等方面的差异性.结果 646例患儿中SPT阳性率81.73%,变应原分布以屋尘螨、粉尘螨为主;阳性病例在性别及检测年、月份分布上的差异无统计学意义(P>0.05);年龄较小组(3~5岁组)的SPT阳性率较低(P<0.05).结论 尘螨为上海地区变应性鼻炎患儿的的主要变应原,致敏阳性率常年维持较高水平,提示了加强尘螨的长期管理在防治儿童变应性鼻炎方面的重要意义.%Objective To determine the features of allergens in children with allergic rhinitis during the recent 5 years of Shanghai, and provide clinical reference for local management strategies. Methods Children with primary diagnosis of allergic rhinitis in shanghai area were given skin prick test (SPT) from June, 2005 to May, 2010. The positive rates were calculated and further analysed in regarding to age, gender, testing months. Results Among 646 children, the total positive rate of SPT was 81.73%. Dermatophagoides farinae (Der. F) and Dermatophagoides pteronyssinus (Der. P) were identified as the mostly responsible allergens. Among the positive subjects, the younger group showed a lower SPT positive rate than the elder (P0.05), but no significant difference was found between the gender, years, or testing months (all, P>0.05). Conclusion Dust mite was the most common allergen in children with allergic rhinitis in Shanghai area, and its sensitization rate sustained constantly a high level around the year, suggesting the importance of long-term mite control during the management of children with allergic rhinitis in Shanghai area.

  15. Analysis of Outpatient Cases in Center of Allergy and Immunotherapy, The General Hospital of Shenyang Military Comonand between 2001 and 2010%2001-2010年沈阳军区总医院变态反应门诊病例分析

    Institute of Scientific and Technical Information of China (English)

    宋薇薇; 林小平; 柴若楠; 仲欢欢; 吕智慧; 冯晓娟

    2012-01-01

    目的 研究沈阳地区2001-2010年变态反应门诊常见病种及发病特征,为当地过敏性疾病的防治提供科学依据.方法 应用本科信息数据库系统,对2001年-2010年本院变态反应门诊就诊的病例进行回顾性统计分析.结果 就诊病种占第一位的为哮喘(44492例,占43%),其次为鼻炎(28070例,占27%)、荨麻疹(12065例,占12%),其他常见病种有花粉症(4652例,占4.5%)、皮炎(3515例,占3.4%)、湿疹(3411例,占3.3%)、紫癜(1964例,占1.9%).引起过敏性疾病的主要过敏原依次为屋尘螨(77.8%)、粉尘螨(75.1%)、蒿草(40.5%)、豚草(22.3%)、狗毛(10.9%)、猫毛(8.5%)、树木(4.1%)、向日葵(3.2%)、葎草(1.95%).7-9月份是过敏性疾病的高发季节.结论 沈阳地区变态反应门诊最常见的病种为哮喘、鼻炎、荨麻疹.尘螨是引起过敏性疾病的主要过敏原,过敏性疾病的患病人数有明显的季节性波动特征.%Objective To investigate the spectrum and characteristics of common allergic diseases in clinic of allergy department in Shenyang, forming the scientific basis of management and prevention of allergic diseases in this area Methods The data of patients were retrospectively analyzed, who attended clinic of allergy department in our hospital since 2001 to 2010, based on the database of hospital information system. Results The most common allergic disease was allergic asthma ( 44 492 cases, 43 % ), followed by rhinitis ( 28 070 cases, 27% ) and urticaria (12 065 cases, 12% ), and the other common diseases were hay fever (4 652 cases, 4.5% ), dermatitis ( 3 515 cases, 3.4% ), eczema ( 3 411 cases, 3.3% ), purpura (l 964 cases, 1.9% ). The major allergens induced allergic diseases according to priority were Dermatophagoid.es pteronyssinus ( 77.8% ), Dermatophagoid.es farina ( 75. 1% ), mugwort pollen ( 40. 5% ), ragweed pollen ( 22. 3% ), dog hairs ( 10. 9% ), cat hairs ( 8. 5% ), tree pollens ( 4. 1% ), sunflower pollen ( 3. 2% ) and

  16. Prevalencia de la sensibilización a tres ácaros domésticos en la población infantil alérgica de un consultorio médico Prevalence of sensitization to three house dust mites in the allergic infantile population of a family physician's office

    Directory of Open Access Journals (Sweden)

    Mayda González León

    2005-04-01

    Full Text Available Se realizó un estudio con el objetivo de investigar si la sensibilización a estos ácaros ( Dermatophagoides pteronyssinus [ Dp ], Dermatophagoides siboney [ Ds ] y Blomia tropicalis [ Bt ] constituye un problema de salud en nuestra población infantil, pues el estudio de los problemas alérgicos ha identificado a los ácaros domésticos como los principales agentes causales de asma y otras afecciones. Se seleccionaron 32 niños alérgicos de nuestro consultorio que se clasificaron según su grado de exposición al polvo doméstico. A todos se les realizó la prueba por punción cutánea en el brazo derecho, y se utilizaron 3 extractos alergénicos estandarizados Valergen Ò ( Blomia tropicalis, Dermatophagoides siboney y Dermatophagoides pteronyssinus de producción nacional (BIOCEN. La prevalencia de la sensibilización fue mayor de forma significativa (pA study was conducted aimed at investigating if the sensitization to these dust mites (Dermatophagoides pteronyssinus [Dp], Dermatophagoides siboney [Ds] and Blomia tropicalis [Bt] is a health problem in our infantile population, since the study of the allergic problems has identified the house dust mites as the main agents causing asthma and other affections. 32 allergic children of our office were selected and classified according to their degree of exposure to house dust. All of them underwent the skin test in the right arm. 3 standardized allergenic extracts ValergenÒ (Blomia tropicalis, Dermatophagoides siboney y Dermatophagoides pteronyssinus of national production (BIOCEN were used. The prevalence of sensitization was significantly higher (p<0.05 for all the dust mites in patients with a greater exposure to house dust compared with those with lower exposure to it. A higher sensitization was attained for the dust mites of the genus Dermatophagoides. The extract of Dp produced a wheal with a mean size greater than the one caused by other products.

  17. Characteristics of aeroallergens of children with allergic rhinitis in Foshan%佛山地区儿童变应性鼻炎患者吸入性变应原特点分析

    Institute of Scientific and Technical Information of China (English)

    于青青; 王跃建; 唐隽; 刘悦胜; 刘美华; 马新苗

    2016-01-01

    Objective To investigate the distribution of aeroallergens in children with allergic rhinitis (AR) in Foshan city and to analyze the relevant factors.Methods 562 children with AR treated at our outpatient department from September,2012 to September,2015 underwent skin prick tests (SPT) with allergopharma skin prick kit,which included 11 kinds of standardized common aeroallergens.The positive rate of variety of allergens was calculated.Their gender,age,family history,and histories of asthma and eczema were analyzed.Results The total positive rate of aeroallergens was 82.56% (464/562).The positive rates of dermatophagoides pteronyssinus (80.64%),dermatophagoides farina (78.66%),and tropical mites (59.31%) were the top three and the positive rates of dog hair (15.36%),cat hair (12.63%),and Blattella germanica (12.32%) came next.Among the positive patients,16 cases (3.30%) were allergic to only one aeroallergen,39(8.02%) to two,and 431 (88.68%)to three or more than three.The positive rate of SPT was different in three different age groups and the highest one was that of the 10-14 years old group (90.18%),that of the 7-10 years old group (87.57%) came next,and that of the 3-7 years old group (79.73%) was the lowest,but with no statistical differences between each two groups (x2=2.452,P>0.05).There was statistical difference in the positive rate of SPT between the female group and the male group (x2=7.195,P<0.05).The positive rate correlated with family history and histories of eczema and asthma.Conclusions The main aeroallergens in children with allergic rhinitis in Foshan city tested by SPT are dermatophagoides pteronyssinus,dermatophagoides farina,and tropical mites.The positive rate of aeroallergens correlates with the sex and age of children.The positive rate of children with family history and the histories of asthma and eczema is much higher than that of the control group.Early skin prick test is helpful for the early diagnosis and

  18. Child allergen investigation with it′s prevention and cure%儿童变应原调查及防治对策

    Institute of Scientific and Technical Information of China (English)

    孙弢; 汤华平; 顾晓玲; 陆敏; 马泽南

    2011-01-01

    Objective To investigate the allergens category in Suzhou Wuzhong district , and to provide the scientific proof for the prevention and cure for the local child allergy diseases . Methods 220 children with allergy diseases were included in the study and performed skin prick test (SPT) on 14 kinds of inhalation allergens and 4 kinds of food allergens. Results 81.82% (180) were demonstrated to be positive, among 18 kinds of allergens, mostly were dermatophagoides pteronyssinus and dermatophagoides farinae,and then were dog epidermis ,cat epidermis,mycete and ragweed. Inhale group:Mostly were positive to dematophagoides ,70. 91% ( 156 cases) , among which 51. 52% were 3 months to 3 years old,75. 76% were from 3 to 7 years old,and 85.45% were above 7 to 14 years old,the difference among the three year groups was significant(P 0. 05 ). Food group: 10. 91 % ( 24 cases ) were allergic to egg, among which 33. 33% were below 3 years old,l. 30% were above 3 years old,there was significant different between the two groups (P <0. 01). Conclusions Most of the children with allergy diseases in Wuzhong district are allergic to two or more allergens, the most common allergens are dermatophagoides pteronyssinus and dermatophagoides farinae ,the allergy rate increase with age, and have nothing to do with the gender ;the pet dog covers a quite large portion should be given high concern . The rate of food allergy is quite low , most of them are among the infants, and descend with age. The treatment can be taken with targetable and effective prevention and cure measures ,such as avoiding,quiting,replacing,removing etc. The SPT testing result also has the great guiding significance for the desensitization therapy . SPT method is simple, convenient and economic for clinical expansion.%目的 检测苏州市吴中区儿童变应原种类,为本地区儿童变态反应性疾病的防治方案提供科学依据.方法 应用阿罗格点刺液,对220 例变态反应性疾病患儿进行14

  19. Analysis of Detecting Aeroallergen Specific IgE and IgE Level of Patients with Allergic Rhinitis in Nanjing%南京地区变应性鼻炎患者吸入性变应原特异性IgE和总IgE的检测分析

    Institute of Scientific and Technical Information of China (English)

    王艾丽; 严孝岭; 刘国瑞; 李梅; 虞伟

    2012-01-01

    %) , dermatophagoides farinae (81. 2%) , cat-dog epithelia (38. 46%) , cockroach(25. 36%) , pollen mixture (13. Ll%),mugwort(ll. 97%) ,ragweed(ll. 11%),plane tree(7. 12%),mould mixture (6. 27%) and birch(1. 14%). Conclusion The total IgE level in junior group was significant elevated and the sensitive to aeroallergen (dermatophagoides pteronyssinus, dermatophagoides farinae and cat-dog epithelia) was also reinforced. The immunoreactionin of hyper-forty paient group was decreased and It would affect total IgE production. The main aeroallergens (over 20%) of induction different age patients with allergic rhinitis in Nanjing area were mites,cat-dog epithelia and cockroach.

  20. 变应性鼻炎患者3292例变应原谱变化分析%Analysis of allergen variation for 3292 patients with allergic rhinitis

    Institute of Scientific and Technical Information of China (English)

    彭晓林; 张金梅; 王丽生; 时文杰; 孙沛湧; 张玉庚; 林鹏

    2013-01-01

    Objective To investigate the variation of common allergens in patients with allergic rhinitis in recent 4 years in Tianjin First Center Hospital.Methods The medical records of skin prick test on 3292 patients with allergic rhinitis between 2009 and 2012 were restrospectively analyzed.The changing trend of various allergens in 4 years and distribution differences were compared.The differences of the top 5 allergens in under age group,adult group and different gender group were further analyzed by SPSS 19.0 software.Results The positive rate of dermatophagoides farinae was increasing year by year,from 45.1% in 2009 to 66.3% in 2012,and the positive rate of dermatophagoides pteronyssinus increased from 42.0% in 2009 to 58.6% in 2012,the difference was statistically significant (x2 value was 68.70,41.55,all P <0.01).The positive rate of dermatophagoides farinae and dermatophagoides pteronyssinus in adult group and male group showed significant upward trend year by year (x2 value was 75.85,69.93,274.25,42.62,all P <0.01),but not in adult group and female group.The positive rate of quinoa,mugwort and humulus scandens decreased year by year between 2009 and 2011 (x2 value was 22.08,11.64,203.19,all P <0.01),but increased again in 2012 (x2 value was 21.55,29.38,12.40,all P < 0.01).Conclusions There is a tendency of change of allergens in patients with AR.This phenominon may be helpful for doctors to choose the type of skin prick liquid.%目的 了解天津市第一中心医院近4年来变应性鼻炎(allergic rhinitis,AR)患者变应原谱的变化情况.方法 回顾性分析2009-2012年确诊为AR的3292例患者皮肤点刺试验结果,研究各变应原阳性率4年来的变化趋势,并进一步分析前5位变应原在未成年人组、成年人组以及不同性别组的变化情况,以SPSS 19.0软件对数据进行统计学分析.结果 粉尘螨、屋尘螨变应原阳性率呈逐年增高趋势,分别由2009年的45.1%、42.0%上升至2012

  1. Allergy-related Evidences in Relation to Serum IgE:Data from the China State Key Laboratory of Respiratory Disease, 2008-2013

    Institute of Scientific and Technical Information of China (English)

    SUN BaoQing; CHEN DeHui; ZHENG PeiYan; HUANG HuiMin; LUO WenTing; ZENG Guang Qiao; ZHANG XiaoWen

    2014-01-01

    ObjectiveTo investigate the serum total IgE (tIgE) and specific IgE (sIgE) to common allergens among allergic patients in Guangzhou, China. Methods7 085 patients were examined for tIgE and sIgE to 15 allergens, based on the protocols of reversed enzyme allergosorbent test and the sandwich enzyme-linked immunosorbent assay. Results3 758(53.04%) patients tested positive for tIgE, and 4 640(65.49%) for sIgE.Der pteronyssinus,Der farinae, eggs, and cow’s milk were the most common allergens leading to higher positive rates of sIgE responses. Several peaks of sensitization were:Der pteronyssinus,Der farinae, and Blomia tropicalis at age 10-12; cow’s milk at age below 3; eggs at age 4-6. The mean level and positive rate of tIgE tended to increase in subjects sensitized to more allergens. Sensitization toDer pteronyssinus (OR, 1.6;P<0.05),Der farinae (OR, 1.5;P<0.05),Blomia tropicalis (OR, 1.4;P<0.05), Blattella germanica (OR, 1.5;P<0.05), cow’s milk (OR, 1.3;P<0.05), and soy beans (OR, 2.0;P<0.05) were independently correlated with allergy-related conditions in preliminary diagnosis. ConclusionThe major allergens in Guangzhou includeDer pteronyssinus, Der farinae, cow’s milk, and eggs. Sensitization to these allergens appears to be predictors of allergy-related disorder.

  2. Tribolium confusum (confused flour beetle, rice flour beetle)--an occupational allergen in bakers: demonstration of IgE antibodies.

    Science.gov (United States)

    Schultze-Werninghaus, G; Zachgo, W; Rotermund, H; Wiewrodt, R; Merget, R; Wahl, R; Burow, G; zur Strassen, R

    1991-01-01

    Specific IgE to proteins from Tribolium confusum (TC), a flour beetle, was detected in 9/125 sera of subjects exposed to rye and wheat flour. TC RAST was not inhibited by Dermatophagoides pteronyssinus, rye or wheat flour. Immunoblot experiments showed specific binding to three proteins from adult TC or pupae, not present in rye or wheat flour. These findings suggest that TC might act as an occupational allergen in a proportion of bakers.

  3. The Effect of Stimuli on Basophil-Mediated Atopic Responses During Asthmatic Lying-In Women and in Newborns

    OpenAIRE

    Yang, Ling; Guo, Yin-Shi; Jiang, Jin-Qi; Guo, Xue-jun; Yi-ping XU; Tian, Ye; Xiong, Ying; Han, Li

    2012-01-01

    Morbidity from allergic diseases is increasing. Basophils play a critical role in systemic anaphylaxis and chronic allergic inflammation. The prenatal environment must be regarded as a possible early risk factor for allergic diseases in children. Our objective was to determine if basophils harvested from neonates genetically predisposed to atopic disease had different levels of CD63 expression and IL-4 release properties in response to various stimuli (peptidoglycan, Dermatophagoides farinae,...

  4. How relevant are house dust mite-fungal interactions in laboratory culture to the natural dust system?

    Science.gov (United States)

    Hay, D B; Hart, B J; Pearce, R B; Kozakiewicz, Z; Douglas, A E

    1992-11-01

    Both house dust and house dust mites Dermatophagoides pteronyssinus contained a wider range of fungi than laboratory mite cultures. In total, nine species of fungi were isolated from D. pteronyssinus in house dust, and these included three xerophilic species (Eurotium amstelodami, Aspergillus penicillioides and Wallemia sebi) commonly found in laboratory cultures of D. pteronyssinus. It is concluded that mites do interact with a similar range of fungi in natural dust and in laboratory culture, but that the diversity of fungal species in the laboratory is reduced and the density of individual fungal species in culture exceeds that of house dust. In a second experiment, dust samples were incubated at room temperature with 75% relative humidity. The diversity of fungi invariably declined from up to 13 genera to the few species recorded in laboratory culture. This suggests that the dominance of xerophilic fungi in laboratory mite rearings is mediated primarily by low relative humidity, and the exclusion of air-borne spores.

  5. 重庆地区过敏性疾病儿童吸入性变应原分布特点%Distribution characteristics of inhaled allergen of 13 841 cases with allergic disease in Chongqing

    Institute of Scientific and Technical Information of China (English)

    李红梅; 李博; 罗征秀; 罗健; 蒋永慧; 刘恩梅

    2012-01-01

    Objective:To explore the distribution characteristics of inhaled allergen in Chongqing area and to provide references for preventing allergic diseases. Methods-.All cases were collected from the Children's Hospital Affiliated to Chongqing Medical University from May 2005 to December 2009.13 841 children with allergic diseases underwent skin prick test(SPT). The distribution characteristics were analyzed by SPSS 17.0. Results-.The total positive rate of allergen was 80.3%, of which Dermal ophagoides farinae (Der f) was the highest (67.6%),then followed by Dermatophagoides pteronyssinus(Der p)(67.5%) and Blomia tropicalis(42.8%). From May 2005 to December 2009, the positive rate and positive degree of the 3 kinds of mites were stable except in 2006. With the age increasing, the positive rate ascended in mites (Der f,Der p, Blomia tropicalis), American cockroach, Blatella germanica.cat hair and dog dander,but Pollen I ,Mycetes IV and ragweed showed opposite tendency. The 13 categories sensibiligen could be divided into 4 clusters;Der p and Der f,Blomia troplis,Blatella germanica.and others. Conclusion;Dermatophagoides farinae,Dermatophagoides pteronyssinus and Blomia tropicalis are the most prevalent allergens in Chongqing area in the past 5 years. The different age groups show different reactions in SPT. The clinical characters of Blomia troplis are different from Der p and Der f, and specific immune therapy that points to Blomia troplis in subtropical areas should be paid attention.%目的:探讨重庆地区儿童过敏性疾病患者吸入性变应原特点,协助过敏性疾病预防及治疗.方法:对重庆医科大学附属儿童医院2005年5月-2009年9月共13 841例过敏性疾病患儿行皮肤点刺试验(Skin prick test,SPT),应用SPSS17.0对数据进行统计学处理,分析吸入性变应原特点.结果:13 841例患儿吸入性变应原皮肤点刺试验阳性率80.3%,其中阳性率最高的变应原依次为粉尘螨67.6%(9 356例)、屋尘螨67

  6. [Sensitization to three species of mites in allergic patients from the coastal area of Havana city].

    Science.gov (United States)

    Almarales, Raúl L Castro; Castelló, Mirta Alvarez; Díaz, Mercedes Ronquillo; Canosa, José S Rodríguez; Gómez, Iris García; León, Mayda González; Domínguez, Irene Enríquez; Rosado, Alexis Labrada; Viltre, Bárbara I Navarro; Díaz, Yunia Oliva; Morejón, Maytee Mateo

    2009-01-01

    Sensitization to three allergic mite species (Dermatophagoides pteronyssinus, Dermatophagoides siboney and Blomia tropicalis) from house dust in Cuba has been reported in different studies. To assess allergic sensitization to these mites in a north coast area of Havana city. A total of 210 allergic patients were selected, classified in two groups: from 1 to 14 years, and from 15 to 55 years. Skin Prick Test (SPT) was performed on all patients using standardized allergenic extracts VALERGEN-BT (B. tropicalis), VALERGEN-DS (D. siboney) and VALERGEN-DP (D. pteronyssinus) manufactured by BIOCEN, Cuba. The test was considered positive when the average wheal diameter was greater or equal to 3 mm. Both groups showed higher sensitization to D. pteronyssinus (>78%), while for the remaining mites it was below 60%. In patients older than 14 years, the sensitization was apparently higher, although not statistically significant (p > 0.05). Nevertheless, the skin reactivity in this group was significantly greater (p < 0.05) for the three allergens. Unusually, mean wheal size in both groups was significantly superior toward B. tropicalis (p < 0.05). In spite of being D. pteronyssinus the mite showing the highest sensitization, Blomia tropicalis showed to be also an important aeroallergen with more than 50% sensitization and greater skin reactivity. Therefore, in our population it would be advisable to perform SPT with the three mites extracts, simultaneously and to combine it in allergen-specific immunotherapy.

  7. Immunophenotyping of the cutaneous cellular infiltrate after atopy patch testing in cats with atopic dermatitis.

    Science.gov (United States)

    Roosje, P J; Thepen, T; Rutten, V P M G; van den Brom, W E; Bruijnzeel-Koomen, C A F M; Willemse, T

    2004-10-01

    Cats with spontaneously occurring atopic dermatitis have clinical and immunocytochemical characteristics compatible with these in humans with atopic dermatitis (AD). The atopy patch test (APT) has proven to be a valuable tool in elucidating the disease process in humans. Additionally, the APT is very specific and bypasses the problem of conflicting results due to differences in chronicity of lesions of AD patients. We adapted the APT for use in cats to explore the suitability of the APT as a tool to study the onset of allergic inflammation in cats with atopic dermatitis. APT were performed in AD cats (n = 6) and healthy cats (n = 10). All cats were patch tested with two allergens in three different dilutions and a diluent control. The allergens for the APT were selected from positive intradermal test and /or prick test results and consisted of: Dermatophagoides farinae, D. pteronyssinus, Tyrophagus putrescentiae, and a grass pollen mixture. APT were read after 10, 24 and 48 h, and punch biopsies for immunohistochemical evaluation were collected at these time points. Macroscopically positive APT reactions were observed in three out of six cats at 24 and/or 48 h with allergen concentrations of 25,000 and 100,000 NU/ml. Reactions were not observed at negative control sites and neither in control animals. A significantly increased number of IL-4+, CD4+, CD3+, MHC class II+ and CD1a+ cells was found in one AD cat with positive APT reactions. Five out of six AD cats had significantly increased IL-4+ T cell numbers at 24 and/or 48 h. Our data indicate that in cats, macroscopically positive patch test reactions can be induced, which have a cellular infiltrate similar to that in lesional skin. We found a high specificity and a macroscopically positive APT reaction in half of the cats, which is similar to what is seen in humans. Hence, the APT in cats might be a useful tool in studying the immunopathogenesis of feline atopic dermatitis.

  8. 2230例变应性疾病患者血清特异性抗体测定分析%Analysis of serum specific IgE in 2 230 patients with allergic diseases

    Institute of Scientific and Technical Information of China (English)

    赵鸿芬; 李小霞; 赵京莉

    2011-01-01

    Objective To detect serum specific IgE in allergic diseases for clinical diagnosis and therapy. Methods The serum specific IgE and total IgE ( slgE) were detected by enzyme immunoassay in 2 230 patients. Results The dermatophagoides farinae (28.3% ) and pteronyssinus( 26. 6% ) were the most common allergen in 2 230 patients, followed by Artemisia(14. 9% ) , cat hair dander(9% ) , beef and mutton(9% ), cockroaches(8.3% ) , seafood(8. 1% ), fungi(6.6% ) , milk(6. 1% ), short ragweed(5.6% ), peanuts, cashew nuts(4.6% ), elm, sycamore, willow, cottonwood(3. 8% ) , egg white(3. 8% ) , soybeans(3. 1% ). The positive rate of total IgE was 46. 9%. Conclusion The detection of serum specific IgE and total IgE can help to find the corresponding clinical allergy, and provide evidences for prevention, diagnosis and therapy of allergic diseases.%目的 检测变应性疾病血清特异性抗体筛选相关过敏原,为临床诊断和治疗提供依据.方法 采用过敏原检测系统,对2 230例变应性疾病患者的血清特异性免疫球蛋白E( immunoglobtlin,IgE)及总IgE水平进行了检测.结果 2 230例变应性疾病患者中过敏原以屋尘螨(28.3%)、粉尘螨(26.6%)为最高,其后依次为蒿(14.9%)、猫毛皮屑(9%)、牛羊肉(9%)、蟑螂(8.3%)、鱼虾蟹(8.1%)、霉菌(6.6%)、牛奶(6.1%)、矮豚草(5.6%)、腰果花生(4.6%)、榆、梧桐、柳、三角叶杨(3.8%)、鸡蛋白(3.8%)、黄豆(3.1%);总IgE阳性率为46.9%.结论 血清特异性IgE及总IgE的检测分析可以帮助临床寻找相应过敏原,为变应性疾病的预防、诊断和治疗提供依据.

  9. PRUEBAS CUTÁNEAS, CON EXTRACTOS ALERGÉNICOS DE ÁCAROS, EN PACIENTES ASMÁTICOS DE LAS CIUDADES DE MACHALA (ECUADOR Y DE CAMAGÜEY (CUBA

    Directory of Open Access Journals (Sweden)

    Rodríguez Santos Olimpio

    2011-01-01

    Full Text Available Background: The extracts of mites Dermatophagoides pteronyssinus, Dermatophagoides siboney and Blomia tropicalis produced in Cuba have been approved and registered for diagnosis and immunotherapy. Objective: Compare performance indicators of the prick test, in the cities of Machala and Camaguey, with extracts of mites developed at the National Center for Biological Preparations. Materials and methods: 40 asthmatics aged 5 years and under 60 Clinic "Medical Tower City of Machala and Camagüey Previsora respectively. Equal number of healthy volunteers of each of the services. Testing was performed in the anterior forearm, applying a drop of each extract at a concentration of 20 000 BU and monitoring of histamine and diluent solution. It punctured the skin through each drop, was read at 15 minutes, being positive when the wheal diameter was less than 3 mm. Results: Sensitivity to Dermatophagoides pteronyssinus, 77, 50 in Camaguey and 72.50 Machala and specificity of 80.00, for the Cuban city and 82.50 for Ecuador. Positive test to Dermatophagoides siboney in Camaguey n = 28 and in Machala n = 9 (p <0.05 in Camagüey sensitivity 70, 00 and 22.50 in Machala, while specificity was 82.50 and 95, 00 respectively. The sensitivity values were 72.50 Blomia tropicalis and specificity 70, 00 and 77, 50, and 85.00 respectively in Camaguey and Machala. Conclusions: The results of statistical indicators attest to the efficacy of mite prick test for diagnosing asthma.

  10. 牛奶过敏儿童外周血白细胞和免疫球蛋白分析%Study on Peripheral-blood Leucocytes and Immunoglobulins in Children with Cow's Milk Allergy

    Institute of Scientific and Technical Information of China (English)

    邝婉仪; 伍绍国; 林爱君; 关小珊; 黄裕游; 黄钰君

    2011-01-01

    Objective To investigate the levels of peripheral-blood leucocytes and immunoglobulins and to observe constituent ratio of allergens in children witb cow's milk allergy so as to provide evidence for doctors to diagnose and treat the disease more reasonably. Methods 341 children with cow's milk allergy and 192 healthy children as control group were enrolled in our study,35 kinds of serum allergen specific IgE, serum total IgE,IgG,IgA,IgM and complement C3,C5 were measured. Then data obtained above were analysed statistically. Results ①The level of eosinophilic granulocyte which was 2. 85 % (1. 15% ~5. 98%) by median in hildren with cow's milk allergy was significantly higher than that in the control group which was 1. 90% (1. 00%~ 4. 10%) (P<0. 05) The level of serum total IgE (g/L) which was 260 (107 ~535. 5) by median was statistically higher than that of the control group[27. 3(10. 2~47. 0)](P<0. 05). ②The most seven higb positive rate among allergen specific IgE combined with cow's milk allergy at the same time were as follows:Dermatophagoides farinae ( 53. 37 %), Dermatophagoides pteronyssinus (52. 25%) , egg (40. 45% ) , cheese (39. 33 %), dog hair (24. 72 %) ,soybean ( 23. 03 %),beef (18. 54%). Conclusion Cow's milk allergy is consistent with type Ⅰ allergy, which is combined with other allergies easily at the same time and children with cow's milk allergy should evade them.%目的 了解牛奶过敏儿童外周血白细胞及免疫球蛋白水平,以及并发其它过敏的变应原的种类,为临床采取合理的诊疗提供科学依据.方法 对341例牛奶过敏儿童和192例健康儿童血清35种过敏原特异性IgE、血清免疫球蛋白IgE,IgG,IgA,IgM和补体C3,C4检测并进行统计学分析.结果 ①牛奶过敏患儿血细胞分析中嗜酸性粒细胞水平中位数2.85%(1.15%~5.98%),明显高于健康对照组中位数1.90%(1.00%~4.10%),P<0.05;免疫球蛋白测定中IgE水平中位数260 g

  11. Analysis of Allergen Skin Prick Tests and Serum Specific IgE in Children with Eczema%湿疹患儿变应原皮肤点刺试验及血清特异性IgE检测结果分析

    Institute of Scientific and Technical Information of China (English)

    陈戟; 刘晓依

    2012-01-01

    Objective To evaluate the clinical application of allergen skin prick tests (SPT) and serum - specific IgE(sIgE) levels in children with eczema. Methods All 605 children with eczema underwent either SPT ( 233 cases) or detection of slgE levels (372 cases ) randomly. According to ages,they were divided into 3 age groups;children under 3 years old,3 to 6 years old and over 6 to analyze the results of positive rates of allergens with 2 methods were compared. Results There were 163 cases (70% ) out of 233 children SPT positive. Among them, the common aeroallergens and food allergens were dermatophagoides farinae ( DF) , dermatophagoides pteronyssinus ( DP) , fungi, egg, cod, milk, peanut and shrimp. There were 291 cases out of 372 children total IgE positive ( 78. 2% ) , and 257 eases were slgE positive (69.1% ). Among them, the common aeroallergens and food allergens were DP, fungi, house dust, dog and cat dander, milk, white egg, shrimp, crab and cashew nut. Comparing the same allergens between SPT and slgE levels, there were no significant difference in positive rates of allergens,except ragweed,dog dander,milk and fish among 3 different groups. Conclusions Both SPT and slgE levels are useful tests to i-dentify potentially significant allergens in children with eczema. SPT is the preferred method for allergen test based on it's quick and easy.%目的 评价变应原皮肤点刺试验(SPT)和血清特异性IgE(sIgE)检测方法在儿童湿疹中的临床应用.方法 对605例湿疹患儿随机进行变应原皮肤点刺试验和血清特异性IgE检测.其中SPT 233例,sIgE 372例,根据年龄分为<3岁组,3~6岁组和>6岁组,分析比较不同年龄组间2种检测方法所得变应原sIgE阳性率.结果 233例患儿进行SPT检测,163例(70%)阳性,其中最常见吸入性和食物性变应原分别为粉尘螨、屋尘螨、真菌和鸡蛋、鳕鱼、牛奶、花生及小虾.372例患儿进行sIgE检测,291例(78.2%)总IgE阳性,257例(69.1%) sIgE

  12. Fatores de risco, aspectos clínicos e laboratoriais da asma em crianças Risk factors, clinical and laboratory aspects of asthma in children

    Directory of Open Access Journals (Sweden)

    Líllian S.L. Moraes

    2001-12-01

    hypersensitivity skin tests were performed with puncture for the detection of the following allergens: house dust mite, animals, molds, and cockroaches with positive (histamine and negative controls (physiologic solution. A logistic regression model was used to calculate the odds ratio (OR and 95% confidence intervals (95%CI adjusted for risk factors and for confounding factors. RESULTS: Among the risk factors studied, sex, parents' low level of education, low income, length of the breast feeding period, and passive smoking were not associated with the presence of asthma. The domestic exposure to allergens was similar in both groups except for the higher frequency of pets at the homes of control patients (c²=16.9; P < 0.05. Paternal history of rhinitis was the only association with asthma (OR=3.33; 95%CI: 1.03-11.17; P < 0.05. The asthmatic children presented higher frequency of positive reactions to skin tests than the controls, mainly to house dust mites: Dermatophagoides pteronyssinus (69.5%, Dermatophagoides farinae (59.3% and Blomia tropicalis (59.3%; cockroaches: Periplaneta americana (59.3%, and cat: Felis domesticus (37.3%, with OR between 11.2-21.0; P < 0.05. Eosinophilia and serum levels of total IgE were more elevated in the group of asthmatic children (P < 0.05. The positivity of the specific IgE test for Dermatophagoides pteronyssinus and Blomia tropicalis was higher in the cases than in the controls (P < 0.05. The multivariate analysis showed that sensitization to the allergens produced by cockroaches (OR=9.26; 95%CI: 2.59-33.4, animals (OR=3.93; 95%CI: 1.05-14.67 and house dust mites (OR=3.74; 95%CI: 1.18-11.8 were the most important risk factors for asthma. CONCLUSIONS: The sensitization to indoor allergens, mainly to house dust mites, cockroaches, and cats showed a strong association with asthma in this study.

  13. Aero-allergens in canine atopic dermatitis in southeastern Australia based on 1000 intradermal skin tests.

    Science.gov (United States)

    Mueller, R S; Bettenay, S V; Tideman, L

    2000-06-01

    To determine the most relevant aero-allergens involved in canine atopic dermatitis in southeastern Australia and provide information about these aero-allergens to the general practitioner. Dogs presented to the Animal Skin & Allergy Clinic with history and clinical signs of atopic dermatitis were injected intradermally with 38 different allergens and negative and positive control. Intradermal skin tests in 1000 dogs were retrospectively evaluated. One third of all patients reacted to the house dust mite Dermatophagoides farinae. Allergens reacting in more than 15% of the patients were wheat (Triticum aestivum), sweet vernal (Anthoxanthum odoratum), English couch (Agropyron repens), yellow dock (Rumex crispus), Mexican tea (Chenopodium ambrosioides), plantain (Plantago lanceolata), melaleuca (Melaleuca quinquenervia) and peppercorn (Schimus spp). House dust mites are the most common allergens in canine atopic dermatitis in southeastern Australia and D farinae is involved most frequently. However, a number of grass, weed and tree pollens also are involved regularly.

  14. Alpha-actinin is a new type of house dust mite allergen.

    Directory of Open Access Journals (Sweden)

    Su An

    Full Text Available Main indoor allergens for humans are from house dust mites. There are more than 30 allergens in Dermatophagoides farinae but only fourteen allergens have been identified from this mite including Der f 1-3, 6, 7, 10, 11, 13-18, and 22. A native allergen protein (Der f 24, 90 kDa was purified from D. farinae by gel filtration and anionic exchange liquid chromatography combined with IgE immunodetection. Its primary structure was determined by Edman degradation, mass spectrometry analysis and cDNA cloning. Enzyme-linked immunosorbent assay inhibition tests (ELISA-IT, immunoblots, basophil activation test (BAT and skin prick test (SPT were performed to evaluate the allergenicity. It was identified as an alpha (α-actinin containing a CaM-like domain with EF-hand motifs. Der f 24 reacted to sera from 85.4% (35/41 of patients on western blot analysis. It reduced ∼20% sera IgE reactivity to D. farinae extracts on a competitive ELISA. Eighty percent (8/10 of patients with D. farinae allergy showed positive reactions to Der f 24 in skin prick test. The expression of CD63 on basophils from patients was up-regulated by Der f 24 by ∼5.4-fold. Alpha-actinin was identified as a new type of house dust mite allergen. To the best of our knowledge, this is the first report of α-actinin as an allergen.

  15. Alpha-actinin is a new type of house dust mite allergen.

    Science.gov (United States)

    An, Su; Shen, Chuanbing; Liu, Xiaoyu; Chen, Lingling; Xu, Xuemei; Rong, Mingqiang; Liu, Zhigang; Lai, Ren

    2013-01-01

    Main indoor allergens for humans are from house dust mites. There are more than 30 allergens in Dermatophagoides farinae but only fourteen allergens have been identified from this mite including Der f 1-3, 6, 7, 10, 11, 13-18, and 22. A native allergen protein (Der f 24, 90 kDa) was purified from D. farinae by gel filtration and anionic exchange liquid chromatography combined with IgE immunodetection. Its primary structure was determined by Edman degradation, mass spectrometry analysis and cDNA cloning. Enzyme-linked immunosorbent assay inhibition tests (ELISA-IT), immunoblots, basophil activation test (BAT) and skin prick test (SPT) were performed to evaluate the allergenicity. It was identified as an alpha (α)-actinin containing a CaM-like domain with EF-hand motifs. Der f 24 reacted to sera from 85.4% (35/41) of patients on western blot analysis. It reduced ∼20% sera IgE reactivity to D. farinae extracts on a competitive ELISA. Eighty percent (8/10) of patients with D. farinae allergy showed positive reactions to Der f 24 in skin prick test. The expression of CD63 on basophils from patients was up-regulated by Der f 24 by ∼5.4-fold. Alpha-actinin was identified as a new type of house dust mite allergen. To the best of our knowledge, this is the first report of α-actinin as an allergen.

  16. Occurrence of indoor allergens in Saudi Arabia

    Energy Technology Data Exchange (ETDEWEB)

    Sorensen, H.; Gravesen, S.; Lind, P.; Schwartz, B.; Ashoor, A.A.; Maglad, S.

    1985-06-01

    Investigations on indoor airborne allergens in Saudi Arabia were performed by mold cultures and dust analyses by counter-current immunoelectrophoresis. Twenty fungal genera were isolated, with Aspergillus as the most often encountered. Most of the dust-bound fungi found are ubiquitous and common. Antibodies against Dermatophagoides pteronyssinus, cat- cow- and rat dander, and Cynodon dactylon pollen were used in the dust analyses. Animal antigens were found in five of the ten dust samples. House dust mites were extraordinarily rare. Pollen of Cynodon dactylon (Bermuda grass) was present in nearly all the samples, and in a concurrent clinical study this antigen was found to be the most common cause of perennial rhinitis.

  17. Avaliação objetiva do teste cutâneo por puntura induzido por histamina e aeroalérgeno através de fotografia digital e termometria cutânea

    OpenAIRE

    Santos, Rosely Vieira dos

    2011-01-01

    Resumo: Objetivo: Empregar novo recurso na leitura do prick test pela fotografia digital. Método: O estudo avaliou a área da pápula induzida por histamina ou por antígenos de Dermatophagoides pteronyssinus em 80 voluntários. Com a câmera digital mantida a uma distância fixa, fotografias seriadas foram realizadas aos 0, 3, 5, 10, 15 e 20 minutos. Definiu-se o contorno da pápula com filtros de transformação de imagem contidos no software Adobe Photoshop 6.0®. A área selecionada a partir do proc...

  18. Mite antigen and allergen contents of house dust samples.

    Directory of Open Access Journals (Sweden)

    Ishii,Akira

    1988-02-01

    Full Text Available The house dust mite (Dermatophagoides pteronyssinus antigen and allergen contents were measured by enzyme-linked immunosorbent assay (ELISA with enzyme-labelled anti-human IgE and anti-mite rabbit IgG antibodies. Antigen content was high in dust samples from homes of patients with allergy but not in samples from homes of patients with Kawasaki disease or of normal control subjects. Allergen content was high in dust samples from homes of Kawasaki disease patients. However, the values overlapped, and we considered these differences to be of little ecological significance, although the assay method itself is useful.

  19. Padronização e estudo da resposta imune de modelos experimentais de asma utilizando ácaros para o teste de novas drogas

    OpenAIRE

    Lima, Ana Tereza Cerqueira

    2010-01-01

    A asma é uma das doenças crônicas mais prevalentes em âmbito mumdial. Estudos epidemiológicos indicam que os ácaros mais frequentemente envolvidos na asma e na rinite alérgica em países tropicais e subtropicais são, respectivamente, Blomia tropicalis (BtE) e Dermatophagoides pteronyssinus (Dp). Embora ácaros sejam os agentes alergizantes mais importantes, o modelo experimental de alergia, a ovalbumina (Ova), é o mais abundantemente utilizado na literatura para o estudo da asma....

  20. 20万项次过敏原特异性IgE检测结果%Two Hundred Thousands Results of Allergen Specific IgE Detection

    Institute of Scientific and Technical Information of China (English)

    王瑞琦; 张宏誉

    2012-01-01

    Objective To analyze and determine the common inhalant allergens and important food allergens in clinical practice. Methods Base on the allergen-specific IgE( slgE ) results detected by ImmunoCAP system in Department of Allergy, Peking Union Medical College Hospital in the past three years, the test amount, the positive results number, and the positive rate of each allergen were calculated respectively, and the sorting analysis of positive results number was performed. Results 215 210 detection were assayed in the past 3 years, 76% of them were inhalant allergens slgE, 22% were food allergens slgE, and 2% were other allergens ( including drugs and insects et al ) slgE. There were 18 kinds of common inhalant allergens, including Dermatophagoid.es pteronyssinus, Dermatophagoid.es farinae, mugwort, Japanese hop, Altermaria alternate, white ash, mountain juniper, common ragweed, common silver birch, Cockroach ( German ), maple leaf sycamore, cocklebur, hand dander, goosefoot ( Lambs quarters ), and Aspergillus fumigatus, which contributed to 95 % of inhalant allergen sigE positive results. The important food allergens were egg, milk, peanut, soybean, shrimp, crab, grain, nut and fruit. The positive slgE results number of 27 kinds ofinhalant and 42 kinds of food allergens was less then ten in the past 3 years, and no positive result was found in some of them. Phadiatop and fx5 were the most common screening tests used in clinical practice. Conclusions The variety of allergens is great, but the common inhalant and important food allergens are limited in clinical practice. Some allergens are really rare. The common inhalant and important food allergens spectrum of China is in accord with data of foreign publications.%目的 分析临床常见吸入物过敏原和重要食物过敏原的种类.方法 依据北京协和医院变态反应科近3年来应用ImmunoCAP过敏原检测系统所做的过敏原特异性IgE(specific IgE,sIgE)检测结果,统计每种过敏原的

  1. Structure of the house dust mite allergen Der f 2

    DEFF Research Database (Denmark)

    Johannessen, Birthe R; Skov, Lars; Kastrup, Jette S

    2005-01-01

    The X-ray structure of the group 2 major allergen from Dermatophagoides farinae (Der f 2) was determined to 1.83 A resolution. The overall Der f 2 structure comprises a single domain of immunoglobulin fold with two anti-parallel beta-sheets. A large hydrophobic cavity is formed in the interior of...... of Der f 2. Structural comparisons to distantly related proteins suggest a role in lipid binding. Immunoglobulin E (IgE) cross-reactivity between group 2 house dust mite major allergens can be explained by conserved surface areas representing IgE binding epitopes....

  2. Assessment of sensitization to insect aeroallergens among patients with allergic rhinitis in Yazd City, Iran.

    Directory of Open Access Journals (Sweden)

    Mohammad Hassan Bemanian

    2012-09-01

    Full Text Available The  frequency of  allergic diseases such  as allergic rhinitis is considerable in general population. Insect aeroallergens are important allergens which can induce airway inflammation. The aim of this study was to determine the prevalence of sensitization to insect aeroallergens in allergic rhinitis patients in Yazd as a desert city in Iran.A cross-sectional study was undertaken on 95 allergic rhinitis patients who were referred to allergy clinic of Yazd city. Skin prick tests (SPT by standard extracts of three insect aeroallergens including Mosquito,  Corn  moth,  Cockroach  and  two  species of  mites  as common  aeroallergens in allergic rhinitis (Dermatophagoid  Farina, Dermatophagoid Peteronysinus were done.SPT results showed that the most common insect aeroallergens were: mosquito (32.6% followed by corn  moth  (26.3% and cockroach (13.7%.The prevalence of SPT positive response to Dermatophagoid Peteronysinus, Dermatophagoid Farina were 8.4% and 7.4%, respectively. These  results  demonstrated  that  sensitization  to  insect  aeroallergens was significantly more common compared to mites in patients with allergic rhinitis in Yazd city, a city surrounded by deserts.High prevalence of skin reactivity to mosquito and corn moth as insect aeroallergens in Yazd city with hot and dry climate in contrast to humid regions such as north of Iran, where mites are more frequent, indicates differences in the prevalence of aeroallergen reactivity in various areas with different climates. Our  study could highlight the importance of insect aeroallergens for clinicians for better  diagnosis and management of patients with allergic rhinitis.

  3. Der f 35: An MD-2-like house dust mite allergen that cross-reacts with Der f 2 and Pso o 2.

    Science.gov (United States)

    Fujimura, T; Aki, T; Isobe, T; Matsuoka, A; Hayashi, T; Ono, K; Kawamoto, S

    2017-04-25

    Dermatophagoides farinae is a source of airborne house dust mite (HDM) allergens. We elucidated IgE-reactive allergens from D. farinae by two-dimensional immunoblotting-based allergenome analysis, and identified one new allergen, named Der f 35, that possesses IgE-binding capacity comparable to that of Der f 2. The aim of this study was to clarify the allergenic capacity of new HDM allergen Der f 35. We cloned der f 35 from D. farinae mRNA and produced recombinant Der f 35 in Escherichia coli. The IgE-binding capacity of Der f 35 and its cross-reactivity with group 2 allergens from D. farinae and Psoroptes ovis were determined by enzyme-linked immunosorbent assay (ELISA) and ELISA inhibition assays, respectively. The deduced amino acid sequence for der f 35, which possesses the MD-2-related lipid-recognition domain, showed higher identity with group 2 allergens from P. ovis (61.5%) and Blomia tropicalis (50.7%) than with Der f 2 (40.8%). Der f 35 showed IgE-binding frequencies of 77.5% (31/40) for the native form upon allergenome analysis and 51.4% (18/35) for recombinant structure by ELISA. Der f 35 showed cross-reactivity with Der f 2 and Pso o 2 in reaction with HDM-allergic patients' IgE by ELISA inhibition assay. Der f 35 is a candidate major allergen from D. farinae, which is more similar to group 2 allergens from sheep scab mite and storage mites. Der f 35 could be responsible for the cross-reactivity among group 2 mite allergens. © 2017 EAACI and John Wiley and Sons A/S. Published by John Wiley and Sons Ltd.

  4. 哮喘患儿吸入糖皮质激素治疗5年后过敏原变化观察%Allergen sensitization changes in asthmatic children receiving inhaled corticosteroid therapy for five years

    Institute of Scientific and Technical Information of China (English)

    汤正珍; 郑跃杰; 王和平; 宋萍; 刘晓莉; 刘萍; 白大明; 王莉

    2012-01-01

    目的 了解哮喘患儿吸入糖皮质激素(ICS)治疗5年后的过敏原变化情况.方法 对2003-2004年在深圳市儿童医院哮喘门诊登记且登记时均行皮肤点刺实验(SPT)并给予ICS治疗的133例哮喘患儿,进行现场回顾性调查.现场调查包括填写调查表和复查SPT.结果 133例哮喘患儿完成了5年前后的2次SPT检测,5年前121例(91%)SPT阳性,12例(9%)SPT阴性;5年后133例中119例 (89.5%)SPT检测阳性,14例(10.5%)SPT阴性,与5年前比较,差异无统计学意义(P>0.05).5年后吸入性过敏原和食物过敏原的阳性率均无明显变化,但蟑螂和树花粉明显增加(P>0.05),牛奶、鸡蛋明显减少(P>0.05).结论 哮喘患儿5年后过敏率变化不大,吸入性过敏有所增加,食物过敏有减少趋势.屋尘螨和粉尘螨仍然是主要过敏原.ICS治疗与否对过敏情况无明显影响.%Objective To assess the allergen -sensitization changes in asthmatic children receiving inhaled corticosteroid (ICS) therapy for 5 years. Methods . The retrospective study was carried out in 133 asthmatic children receiving treatment of ICS from 2003-2004 in Shenzhen Children' s Hospital. The patients were assessed by questionnaire and skin prick tests (SPT).Results Totally 133 patients finished the SPT before and after five years. The positive rate of SPT in 133 cases was 91% before five years,which was not significantly different(P > 0.05)from that of the patients (89.5%)af-ter five years . There was no significant increase in food and inhalant hypersensitivity (P > 0.05) ,but Cockroach and Tree-pollen increased obviously, and milk and eggs were reduced significantly.Conclusions Allergen sensitization changes in asthmatic children received inhaled corticosteroid therapy for five years are small.Inhalant allergens increase obviously, but the tendency of food hypersensitivity is reduced.Dermatophagoldes pteronyssinus and Dermatophagoides farina are still the main allergen.

  5. Efecto acaricida de una mezcla química

    Directory of Open Access Journals (Sweden)

    E. Egea

    2000-02-01

    Full Text Available

    Introducción: Se investiga el efecto de una nueva mezcla química (ATLASAC para el control de ácaros del polvo doméstico, microorganismos cosmopolitas que conviven con el hombre, reconocidos como importante fuente de alergenos y la principal causa de exacerbación de las enfermedades alérgicas. En el medio ambiente de los hogares colombianos se conoce por reportes científicos la prevalencia de varias especies comunes en otros países: Dermatophagoides pteronyssinus, D. Farinae, Blomia tropicales, etc, asociados con asma y/o rinitis. Por todo ello, en el medio ambiente residencial del paciente es importante establecer medidas locativas tendientes a evitar la exposición y el control químico para reducir o eliminar la fuente de alergenos, en especial, los ácaros. Un recurso fácil de aplicar son los acaricidas, los cuales varían en el grado de efectividad y tiempo de protección. En nuestro medio no se tienen productos aprobados como acaricidas. Por tal motivo, esta investigación pretende llenar un vacío en el medio colombiano con una mezcla química con efecto acaricida, en especial sobre aquellos implicados con las enfermedades alérgicas.

    Metodología: Se seleccionaron 25 pacientes definidos por historia clínica como alérgicos y 50 voluntarios que por pruebas cutáneas se descartó sensibilización a ácaros. Inmediatamente después del ingreso se hicieron muestreos para determinar la presencia de ácaros del polvo doméstico y fundamentalmente para obtener cultivos puros.

    Resultados preliminares: 100 ácaros de

  6. Diversidade da exposição alergênica: implicações na obtenção da eficácia do controle ambiental Diversity of allergen exposure: implications for the efficacy of environmental control

    Directory of Open Access Journals (Sweden)

    Gesmar Rodrigues Silva Segundo

    2009-04-01

    Full Text Available As doenças alérgicas, como a asma, rinite, conjuntivite alérgica e a dermatite atópica têm apresentado um aumento na sua prevalência nas últimas décadas. A relação entre exposição alergênica, sensibilização atópica e desenvolvimento de doenças alérgicas são amplamente descrita na literatura. OBJETIVO: Discutir a dificuldade no controle ambiental da exposição alergênica como parte do tratamento das doenças alérgicas. MÉTODOS: Analisar trabalhos de exposição alergênica realizados com metodologia similar na região central do Brasil, incluindo casas, hotéis, cinemas, carros, táxis, ônibus e transporte escolar. RESULTADOS: Níveis elevados dos alérgenos do grupo 1 de Dermatophagoides pteronyssinus (Der p 1 e de D. farinae (Der f 1, capazes de causar sensibilização e exacerbação de sintomas foram encontrados na maioria dos ambientes estudados em uma larga proporção das amostras, enquanto os alérgenos de animais domésticos atingiram maiores níveis em carros e veículos de transporte escolar. CONCLUSÃO: A diversidade da exposição alergênica mostra a necessidade de uma compreensão da doença alérgica pelos pacientes e familiares, e que as medidas de controle do ambiente doméstico fazem parte de uma estratégia global do tratamento das doenças alérgicas, uma vez que os indivíduos vivem em uma sociedade e não isoladas no interior de seus domicílios.The prevalence of allergic diseases such as asthma, rhinitis, allergic conjunctivitis and atopic dermatitis has increased in the last decades. The relationship between allergen exposure, atopic sensitization and development of allergic diseases is widely described in the literature. AIM: To evaluate measures for reducing allergen exposure as part of the treatment of allergic diseases. METHODS: An analysis was made of previous studies on allergen exposure done with a similar methodology in the central region of Brazil; the study included homes, hotels, cinemas

  7. Hypersensitivity to Aeroallergens in Patients with Nasobronchial Allergy

    Science.gov (United States)

    Balaban, Jagoda; Bijelic, Radojka; Milicevic, Snjezana

    2014-01-01

    ABSTRACT Background: Aeroallergens are the most common causes of allergy. Aim: The aim of this study was to determine hypersensitivity to aeroallergens in patients with nasobronchial allergy. Methods: This retrospective population study included 2254 patients with nasobronchial allergy, from late adolescents to adults. Their response to aeroallergens was assessed by skin prick tests. Results: More patients had rhinitis (72.7%), than asthma (27.6%). Although majority of patients were female, allergy is more common in men than in women (p<0.05). Both groups of patients had the greatest number of positive skin prick tests for Dermatophagoides pteronyssinus (27.5%) and weed pollens (21.9%), followed by grass (18.3%) and tree pollens (10.1%). Ragweed is the most common positive weed pollen in both groups, more in patients with rhinitis (p=0.022). The cocksfoot is the most common grass pollen in rhinitis group (15.3%), but meadow grass (12.6%) in asthma patients. Birch is the most common tree allergen in the both groups. Conclusions: More patients with nasobronchial allergy have rhinitis than asthma. Skin prick tests are usually positive for Dermatophagoides pteronyssinus and weed pollens, followed by grass and tree pollens, and they are more common positive in patients with rhinitis than asthma. PMID:24937928

  8. Clinical Efficacy of Intralymphatic Immunotherapy for Allergic Asthma%淋巴免疫治疗过敏性哮喘的临床疗效

    Institute of Scientific and Technical Information of China (English)

    雍磊; 高春芳; 程相铎; 王俊琦; 李华信; 董学文; 贾丹; 张彩霞; 何静

    2013-01-01

    Objective To evaluate the clinical efficacy and safety of intralymphatic immunotherapy with standardized Dermatophagoides pteronyssinus allergen vaccine for Dermatophagoides pteronyssinus-induced allergic asthma. Methods 72 patients with Dermatophagoides pteronyssinus -induced allergic asthma were randomly divided into intralymphatic immunotherapy treatment group and control group, 36 cases were enrolled in each group. Patients in control group were prescribed budesonide and salbutamol aerosol while patients in intralymphatic immunotherapy treatment group were prescribed above medications and also received 6 ultrasound-guided intra-superficial lymph node injections by using standardized aluminum-adsorbed Dermatophagoides pteronyssinus allergen vaccine ( Alutard ALK, Denmark) every four weeks. The evaluation of clinical efficacy was based on serum Dermatophagoides pteronyssinus slgE, asthma control test (ACT) , pulmonary function test [FEV1% , FEV1/FVC and PEF% ] , medication score on baseline and after treatment, comparing with control group. Adverse reactions were also recorded. Results 32 cases in treatment group completed the study, 2 cases dropped out due to pregnancy and another 2 cases lost. 32 cases in control group completed the study and 4 cases lost. ACT scores, FEV1% , FEV1/FVC sand PEF % values of patients' in intralymphatic immunotherapy group increased significantly, comparing with that of patients in control group. Medication scores and serum Dermatophagoides pteronyssinus slgE of patients in treatment group decreased significantly, comparing with that of pre-treatment and that of in control group. There was no local and systemic adverse reaction in 192 intralymphatic injections among 32 patients. Conclusions Allergen specific ntralymphatic immunotherapy is not only very effective, but also can remarkably shorten the duration of immunotherapy, reduce the number and dose of injection and decrease adverse reaction. Intralymphatic immunotherapy with

  9. The effect of stimuli on basophil-mediated atopic responses during asthmatic lying-in women and in newborns.

    Science.gov (United States)

    Yang, Ling; Guo, Yin-Shi; Jiang, Jin-Qi; Guo, Xue-Jun; Xu, Yi-Ping; Tian, Ye; Xiong, Ying; Han, Li

    2012-08-01

    Morbidity from allergic diseases is increasing. Basophils play a critical role in systemic anaphylaxis and chronic allergic inflammation. The prenatal environment must be regarded as a possible early risk factor for allergic diseases in children. Our objective was to determine if basophils harvested from neonates genetically predisposed to atopic disease had different levels of CD63 expression and IL-4 release properties in response to various stimuli (peptidoglycan, Dermatophagoides farinae, hyperosmotic mannitol). Blood samples were collected from 16 asthmatic and 18 healthy women and their newborns. Peripheral blood basophil histamine was measured using the human basophil degranulation test (HBDT), whereas activation was assessed by flow cytometric measurement of CD63 expression on the cord blood basophil surface. IL-4 levels were quantified by ELISA following allergen stimulation. The basophil degranulation index (DI) in granulocytes harvested from the peripheral blood of asthmatic women was assessed following stimulation with peptidoglycan (PGN), Dermatophagoides farinae (Df ) extract, or hyperosmotic mannitol. The DI was significantly higher in atopic women than in healthy controls. Upregulation of CD63 on the cord blood basophil surface was also detected in response to these stimuli. Basophils purified from the cord blood of neonates born to atopic mothers produced more IL-4 compared to basophils purified from the controls. These data suggested that various stimuli play a role in augmenting allergic reactions via modulation of activated basophil cytokine secretion. It may require new methods to stabilize the basophils in allergic diseases.

  10. Curative effect analysis of sublingual immunotherapy in 62 cases of children with allergic rhinitis%舌下免疫治疗儿童变应性鼻炎62例疗效观察

    Institute of Scientific and Technical Information of China (English)

    沈荣

    2011-01-01

    目的 评价标准化粉尘螨变应原疫苗滴剂舌下含服治疗儿童变应性鼻炎的疗效并初步探索其免疫学机制.方法 选择粉尘螨阳性的儿童变应性鼻炎患者62例,随机分为2组:免疫治疗组31例,给予舌下含服标准化粉尘螨变应原疫苗滴剂脱敏治疗;药物治疗组31例,根据患者情况,给予适量抗组胺药口服,或鼻用类固醇激素局部喷鼻.治疗疗程为2年,治疗后观察患者症状、体征变化,采用视觉模拟量表(VAS)评定疗效,并比较治疗前后血清中总IgE、尘螨特异性IgE的水平.结果 免疫治疗组有效率为80.6%,药物治疗组有效率为64.5%,2组有效率比较差异有统计学意义(P0.05).免疫治疗组治疗前后血清总IgE、尘螨特异性IgE水平比较差异无统计学意义(P>0.05).结论 应用标准化粉尘螨变应原疫苗滴剂舌下含服对变应性鼻炎患者进行免疫治疗能够有效改善变应性鼻炎患儿的症状.%Objective To evaluate the curative effect of sublingual immunotherapy in children allergic rhinitis by standardized dermatophagoides farinae allergen vaccine drops and explore the immunological mechanisms. Methods 62 cases of children with allergic rhinitis whose test result was positive for dermatophagoides farinae were divided into two groups randomly:The sublingual immunotherapy group( 31 cases )which accepted standardized dermatophagoides farinae allergen vaccine drops sublingual immunotherapy and the control group( 31 cases ) which accepted antihistamine drug therapy or local nasal steroid spray. The course of treatment was two years. Evaluating the curative effect by the changes of symptoms and physical signs which were measured by VAS ( visual analogue scale ) and detecting the serum levels of total IgE, specific IgE levels of dermatophagoides farinae. Results The effective rate was 80.6% in the sublingual immunotherapy group, and 64.5% in the control group. Although the symptom score was statistically

  11. The Roles of Malassezia Yeast and House Dust Mites in Atopic Dermatitis with Head and Neck Involvement

    Directory of Open Access Journals (Sweden)

    Gökhan Okan

    2013-03-01

    Full Text Available Objective: The aim of the present study was to evaluate to the effects of Dermatophagoides pteronyssinus, Dermatophagoides farinea and Pityrosporum ovale allergens on atopic dermatitis and to compare the patients with and without head and neck involvement. Moreover, the effect of P. ovale on atopic dermatitis according to different geographic conditions was also determined. Methods: Thirty-one patients with atopic dermatitis, who were admitted to İstanbul Medical Faculty Dermatology Clinic, were enrolled. Skin prick tests were performed for P. ovale and house dust mites (D. pteronyssinus ve D. farinea Results: Ten patients (32.3% showed positive reaction to D. pteronyssinus, nine patients (29% to D. farinea, and nine patients (29% to P. ovale. Head and neck involvement was observed in 22 patients. Head and neck involvement was mostly seen in the patients in the first two decades of life (48%. Eczema was significantly more severe in the patients with head and neck involvement compared to that in those without (p500 IU/mL were significantly higher in the head and neck involvement group (p<0.05. Conclusion: Although the effect of P. ovale on atopic dermatitis in head and neck involvement could not be demonstrated in the present study, it should not be ignored. The results of skin prick test for P. ovale might change according to age and disease severity. Geographical variations of Malassezia species distribution should be kept in mind while evaluating test results. Moreover, it should be remembered that inhalan allergens are also triggering factor in some patients with atopic dermatitis with head and neck involvement.

  12. The hammock: a reservoir of allergens

    Directory of Open Access Journals (Sweden)

    Francisca X. M. Rego

    2011-01-01

    Full Text Available INTRODUCTION: Asthma affects approximately 10% of the world's population. Sensitization to allergens is an important risk factor, and exposure to allergens is associated with disease severity. METHODS: We performed skin tests to evaluate allergen sensitization to mites, cockroaches, cats, dogs, and molds in 73 asthmatic patients. Enzyme Linked Immunosorbent Assay was used to assay the mite and cockroach allergens found in dust from the bedding, hammocks, bedroom floors, living rooms, and kitchens of 29 patients and 14 controls. RESULTS: Fifty patients (68.5% had positive skin test responses. There were positive responses to D. pteronyssinus (52.0%, B. tropicalis (53.4%, T. putrescentiae (15.0%, E. maynei (12.3%, L. destructor (8.2%, B. germanica (20.5%, P. americana (21.9%, Felis catus (10.9%, C. herbarium (2.7%, A. alternata (4.1%, and P. notatun (1.3%. The exposure to mite and cockroach allergens was similar in the patients and the controls. The Dermatophagoides pteronyssinus Group 1 levels were highest in the beds and hammocks. The Blattella germanica Group 1 levels were highest in the kitchens, living rooms and hammocks. DISCUSSION: The positive skin tests to mites, cockroaches and cats were consistent with previous studies. D pteronyssinus was the most prevalent home dust mite, and hammocks were a source of allergens. To improve asthma prophylaxis, it is important to determine its association with mite allergen exposure in hammocks.

  13. Lepidoglyphus destructor acarus in the urban house environment.

    Science.gov (United States)

    Juliá, J C; Martorell, A; Ventas, P; Cerdá, J C; Torró, I; Carreira, J; Guinot, E; Sanz, J; Alvarez, V

    1995-01-01

    To assess the presence of Lepidoglyphus destructor in the household environment of sensitized children living in an urban environment, samples of house dust were collected at the homes where two groups of patients were living, as well as in two bakeries in the city of Valencia, which were taken as a reference. Patients were divided into two groups. Group A included atopic children suffering from rhinitis and/or asthma, who were sensitized to L. destructor, as proven by prick test and specific IgE (CAP). Group B included children with the same features as those included in Group A, who were sensitized to Dermatophagoides pteronyssinus, with prick and CAP tests showing no significant sensitization to L. destructor. The samples of dust were analyzed, and the amounts of Der p I, Der f I, Der II and Lep d I per gram of dust were assessed through a solid-phase ELISA with monoclonal antibodies. In Group A, all patients but two showed a sensitization to D. pteronyssinus by prick test and serum IgE. At the homes of the patients from both groups, significant levels of Dermatophagoides were found. In Group A, only three houses showed levels of L. destructor which were comparable to those found in bakeries. Lep d I was not found in the houses of Group B patients. This means that a sensitization to L. destructor, as assessed with full extracts, is not always an indicator of its presence at the patient's house environment; it may rather refer to cross-reactivity to Dermatophagoides. Thus, availability of the main antigen Lep d I seems necessary to increase the specificity of the allergologic study.

  14. Serological identification of house dust mite allergens in dogs with atopic dermatitis

    Directory of Open Access Journals (Sweden)

    Victor E.S. Cunha

    2012-09-01

    Full Text Available House dust mite antigens have been used for decades to diagnose allergic diseases in humans and animals. The objective of this study was to identify allergens in commercial Dermatophagoides farinae and Blomia tropicalis extracts by immunoblotting using sera from allergic dogs and anti-dog IgE conjugate. The analysis of antigens present in the D. farinae extract (FDA Allergenic using sera from 10 dogs allergic to D. farinae showed that eight sera recognized a band of approximately 102 kDa, eight recognized two bands of 52 to 76 kDa, five recognized one band of approximately 76 kDa, four recognized one band of 31 to 38 kDa, and two recognized one band of 12 to 17 kDa. Immunoblot assays of the B. tropicalis extract (FDA Allergenic using sera from 10 animals allergic to B. tropicalis showed that five sera recognized two bands of 52 to 76 kDa. These results demonstrate the importance of the two house dust mite species for the pathogenesis of canine atopic dermatitis in Brazil. In addition, the results indicate which allergens should be present in allergenic extracts used for diagnosis and allergen-specific immunotherapy.

  15. 广州地区呼吸道疾病患儿16种常见变应原检测分析%Analysis and measurement of sixteen common allergens in children with respiratory disorders in Guangzhou

    Institute of Scientific and Technical Information of China (English)

    孙宝清; 郑佩燕; 王艳萍; 韦妮莉; 陈德晖; 钟南山

    2009-01-01

    of two different age groups. Methods From August 2007 to March 2008, 320 children primarily diagnosed as bronchial asthma, allergic rhinitis or respiratory infections in our respiratory or pediatric department as out-patients were enrolled in the study. In those children, 214 children were no more than 3 years (infants group) and 106 children were more than 3 years (elder children group). Specific IgE (SIgE) antibodies of sixteen common allergens were measured by serologic test with immunocapture methods using ALLERG-O-LIQ-SYSTEM (Dr. Fooke Laboratory, Germany). Results Fifty-nine cases(18.44%) were SIgE negative and 261 cases (81.56%) were SIgE positive. The positive rates of SIgE for 16 common allergens were Dermatophagoides pteronyssinus (36.88%), Dermatophagoidesfarinae (35.31%), tropical mites (24.06%), dog furs (6.56%), cat furs (8.75%), German cockroach (16.56%), honey bee venom (0.94%), house dust (45.63%), whole-eggs (45.94%), milk (46.25%), wheat flour (6.88%), corn flour (1.25%), peanuts (9.06%), soybeans (5.94%), crabs (2.81%) and shrimp (4.38%). The SIgE positive rate was 81.78% in infants group and 81.13% in elder children group(X2=0.02,P=0.89). Allergy to foods (such as milk, whole-eggs) was more common in infants group, while allergy to inhalational allergens (such as dust mites) was more common in elder children group. For dust mite allergies, the SIgE positive rate was higher in elder children group than that of infants group(P<0.05). The elder children group had stronger response more than grade 3 to Dermatophagoides pteronyssinus and Dermatophagoides farinae. The SIgE positive rate in different grades was not significant in infants group. And weak responses mostly less than grade 3 to tropical mites were shown in both groups. For milk and whole-eggs allergies, the SIgE positive rate was higher in infants group than that of elder children group(P<0.05). Responses less than grade 3 to milk and whole-eggs were shown in both groups. Conclusions The

  16. Mite allergoids coupled to nonoxidized mannan from Saccharomyces cerevisae efficiently target canine dendritic cells for novel allergy immunotherapy in veterinary medicine.

    Science.gov (United States)

    Soria, Irene; Alvarez, Javier; Manzano, Ana I; López-Relaño, Juan; Cases, Bárbara; Mas-Fontao, Ana; Cañada, F Javier; Fernández-Caldas, Enrique; Casanovas, Miguel; Jiménez-Barbero, Jesús; Palomares, Oscar; Viñals-Flórez, Luis M; Subiza, José L

    2017-08-01

    We have recently reported that grass pollen allergoids conjugated with nonoxidized mannan of Saccharomyces cerevisae using glutaraldehyde results in a novel hypoallergenic mannan-allergen complex with improved properties for allergen vaccination. Using this approach, human dendritic cells show a better allergen uptake and cytokine profile production (higher IL-10/IL-4 ratio) for therapeutic purposes. Here we aim to address whether a similar approach can be extended to dogs using canine dendritic cells. Six healthy Spanish Greyhound dogs were used as blood donors to obtain canine dendritic cells (DC) derived from peripheral blood monocytes. Allergens from Dermatophagoides farinae mite were polymerized and conjugated with nonoxidized mannan. Nuclear magnetic resonance (NMR), gel electrophoresis (SDS-PAGE), immunoblotting and IgE-ELISA inhibition studies were conducted to evaluate the main characteristics of the allergoid obtained. Mannan-allergen conjugate and controls were assayed in vitro for canine DC uptake and production of IL-4 and IL-10. The results indicate that the conjugation of D. farinae allergens with nonoxidized mannan was feasible using glutaraldehyde. The resulting product was a polymerized structure showing a high molecular weight as detected by NMR and SDS-PAGE analysis. The mannan-allergen conjugate was hypoallergenic with a reduced reactivity with specific dog IgE. An increase in both allergen uptake and IL-10/IL-4 ratio was obtained when canine DCs were incubated with the mannan-allergen conjugate, as compared with the control allergen preparations (unmodified D. farinae allergens and oxidized mannan-allergen conjugate). We conclude that hypoallergenic D. farinae allergens coupled to nonoxidized mannan is a novel allergen preparation suitable for canine allergy immunotherapy targeting dendritic cells. Copyright © 2017 The Authors. Published by Elsevier B.V. All rights reserved.

  17. Desenvolupament de begudes a base de farina de quinoa i sucs de fruites

    OpenAIRE

    Saubi Oriol, Meritxell

    2016-01-01

    Quinoa (Chenopodium quinoa Willd.) is a pseudocereal and is a native plant in the Andean region. Is known for its protein quality with a well balanced amino acids; also, it contains a considerable amount of vitamins, minerals, antioxidants and others nutrients. The main objective of this Bachelor's thesis Degree in Food Engineering has been make a previous develop of a soft drink pasteurized mixed, stable and acceptable organoleptically, with quinoa flour and fruits juices, that could preserv...

  18. Eficiencia de dos pruebas diagnósticas en la determinación de alergia por ácaros en niños

    Directory of Open Access Journals (Sweden)

    Yadira Fuentes

    2008-08-01

    Full Text Available Las enfermedades alérgicas constituyen un problema de salud mundial y los ácaros se encuentran entre sus principales agentes etiológicos. En Cuba se destacan los siguientes: Dermatophagoides pteronyssinus, Dermatophagoides siboney y Blomia tropicalis. Con el objetivo de determinar la eficiencia de la prueba cutánea por punción y la determinación de IgE específica para el diagnóstico de la alergia a estos tres ácaros se desarrolló en el año 2003 un estudio analítico que incluyó 100 niños: 50 no alérgicos y 50 alérgicos que asistieron al Hospital Pediátrico "William Soler" de Ciudad de La Habana. A todos se les realizó la prueba cutánea por punción con tres extractos alergénicos (VALERGEN-DP, VALERGEN-DS y VALERGEN-BT y se les extrajo sangre para la valoración de la IgE ácaro específica; exámenes que mostraron una mayor positividad ante D. pteronyssinus en los niños enfermos. Mientras que en los no alérgicos la prueba cutánea y la IgE para D. pteronyssinus fueron negativas. La prueba cutánea presentó un 100% de especificidad, con una mayor sensibilidad para VALERGEN-DP (88%, extracto con más eficiencia diagnóstica (94%. La IgE expresó los mejores resultados de sensibilidad, especificidad y eficiencia para D. pteronyssinus (78, 100 y 89%, respectivamente. La prueba cutánea por punción fue más eficiente que la determinación de IgE específica para el diagnóstico de las alergias por ácaros. En los enfermos se observó una correlación significativa entre la respuesta cutánea y el nivel de IgE para los ácaros estudiados. Estos datos contribuyeron a mejorar el diagnóstico alergológico y alertan sobre la necesidad de extender el uso de la inmunoterapia específica con vacunas hiposensibilizantes en los niños alérgicos a los ácaros.

  19. Prevalence of common allergens among asthmatic children in Haikou%海口市儿童哮喘变应原流行病学调查研究

    Institute of Scientific and Technical Information of China (English)

    王灵; 陈实; 廖锋; 曾霞; 吴少皎; 谢跃琦; 林涛; 林霞; 陈冰

    2016-01-01

    Objective To investigate the prevalence of allergens in asthmatic children in Haikou. Methods A total of 10 241 children of 0~14 years old were involved and investigated by cluster sampling, in which 324 children were diagnosed as asthma. Allergic sensitization was studied with questionnaire, skin prick tests and specific IgE mea-surements. Results Among the 324 asthmatic children, 224 (69.1%) had allergy history, including 91 cases (28%) of ec-zema, 64 cases (19.8%) of food allergy, 28 cases (8.6%) of drug allergy, 18 cases (5.6%) of cigarette smoke allergy, 19 cases (5.9%) of decoration smell allergy, 13 cases (4.0%) of pet allergy, 11 cases (3.4%) of cooking oil smell allergy, and 9 cases (2.8%) of pollen allergy. A total of 269 asthmatic children (83.0%) showed SPT positive reaction, with 292 cases (90.1%) of Dermatophagoides pteronyssinus (Der p), 289 cases (89.2%) of Dermatophagoides farina (Der f), 277 cases (85.5%) of Blomia tropicalis (Blo t), 64 cases (19.6%) of cockroach, 11 cases (3.4%) of mould, 11 cases (3.4%) of pol-len, 38 cases (11.7%) of cat dander, and 82 cases (25.3%) of dog dander. The specific IgE-positive rate to inhaled aller-gens was 72.2%(235 cases), with 69.8%(226 cases) to mites, which was the highest. The specific IgE-positive rate to food allergens was 28.7%(93 cases), with 25.3%(82 cases) to milk, 9.9%(32 cases) to egg white, 22.8%(74 cases) to crab and shrimp, 9.0%(24 cases) to tropical fruit, and 8.3%(27 cases) to fish. Comparison of inhaled allergen specific IgE-positive rate between different age groups showed that, the positive rate to dust mites, cockroach was significantly higher in 3~7 years old group than 0~3 years old group old, and the positive rate to dust mites, cockroach, dog dander, cat dander was higher in 7~14 years old group than 0~3 years old group. Conclusion House dust mites and Blomia tropicalis are the major allergen sources among asthmatic children in Haikou. Dog dander, cat dander and cockroach are also

  20. 159 Therapeutic Effect and Safety of the Sublingual Immunotherapy With Tropical House Dust Mite Allergen Vaccines in Asthmatic Cuban Adult Patients

    Science.gov (United States)

    Castro Almarales, Raúl Lázaro; Ronquillo, Mercedes; Labrada, Alexis; Castello, Mirta Alvarez; González, Mayda; Rodríguez, José; Enriquez, Irene; Navarro Viltres, Bárbara I; DíazLic, Yunia Oliva; Mateo, Maytee

    2012-01-01

    Background Subcutaneous injection route (SCIT) is burdened with the risk of severe adverse events; therefore, sublingual immunotherapy (SLIT) is being increasingly investigated. The efficacy of SLIT in asthma has been reviewed in a Cochrane meta-analysis. Allergic sensitization to Dermatophagoides pteronyssinus (Dp), Dermatophagoides siboney (Ds) and Blomia tropicalis (Bt) is strongly linked to respiratory allergy and asthma in Cuba (3). These last 2 species are relevant in tropical countries or even only in the Caribbean region (4). Nevertheless, well conducted clinical studies of immunotherapy with standardized allergen vaccines of these particular species are very scarce. Objective This study was conducted to assess the therapeutic effect and safety of allergen therapeutic vaccines of Dermatophagoides pteronyssinus, Dermatophagoides siboney and Blomia tropicalis House-Dust mites (VALERGEN, BIOCEN, Cuba) by sublingual route, in asthmatic patients. Methods Three Double-Blind Placebo-Controlled clinical trials were performed in 40 patients each, showing asthmatic symptoms and positive predominant Skin Prick Test (SPT) to each mite, respectively. Half of subjects were randomized to active group. Treatment consisted of sublingual drops with increasing daily doses for 3 weeks and maintenance doses (2000 BU) twice a week until 12 months. Results Therapeutic effect was assessed after 6 and 12 months using symptoms/medication diary cards, peak expiratory flow (PEF) measures and skin sensitivity to investigated mites. Adverse reactions were classified using the World Allergy Organization scale. The treatment reduced significantly (P < 0.01) clinical symptoms (38%, 95% CI, 33-44) and medication intake (26%, 95% CI, 21-32) with respect to placebo. The skin sensitivity to the allergens decreased also significantly (P < 0.01). The allergen amount needed to induce a positive SPT increased 52-fold. PEF variability decreased also significantly (P < 0.05). The treatment was

  1. Dose of house dust mite antigen (P1) inhaled by infants aged one month

    Energy Technology Data Exchange (ETDEWEB)

    Carswell, F.; Clark, J.; Robinson, P.; Platts-Mills, T.A.

    1983-11-01

    A survey of the habitats occupied by 12 infants of one month of age revealed that approximately 10% of their day was spent in conditions of potential exposure to the major (P1) allergen of the house dust mite, Dermatophagoides pteronyssinus. A respiratory pump which reproduced the minute ventilation of an infant was placed in representative infant habitats. The P1 allergen trapped by the filter in this pump was measured as an estimate of infants' allergen intake. Detectable P1 intake was only present when there was active air disturbance (bed making and vacuuming). The average P1 intake was approximately 3 ng P1/24 hours. Comparison of this P1 intake with that which sensitizes in other situations suggests that it is usually inadequate to sensitize infants.

  2. GALEN skin test study III: Minimum battery of test inhalent allergens needed in epidemiological studies in patients

    DEFF Research Database (Denmark)

    Bousquet, P-J; Burbach, G; Heinzerling, L M

    2009-01-01

    Background: The number of allergens to be tested in order to identify sensitized patients is important in order to have the most cost-effective approach in epidemiological studies. Objective: To define the minimal number and the type of skin prick test (SPT) allergens required to identify a patient...... as sensitized using results of the new Pan-European GA(2)LEN skin prick test study. Method: In a large Pan-European multicenter (17 centers in 14 countries) patient based study, a standardized panel of 18 allergens has been prick tested using a standardized procedure. Conditional approach allowed to determine...... the allergens selection. Result: Among the 3034 patients involved, 1996 (68.2%) were sensitized to at least one allergen. Overall, eight allergens (grass pollen, Dermatophagoides pteronyssinus, birch pollen, cat dander, Artemisia, olive pollen, Blatella and Alternaria) allowed to identified more than 95...

  3. Atopy patch tests in young adult patients with atopic dermatitis and controls: dose-response relationship, objective reading, reproducibility and clinical interpretation

    DEFF Research Database (Denmark)

    Bygum, Anette; Mørtz, Charlotte G; Andersen, Klaus Ejner

    2003-01-01

    The clinical interpretation and reproducibility of atopy patch tests was studied in 23 selected young adult patients with atopic dermatitis and 25 healthy controls using standard inhalant allergens. Non-invasive measurements were used for objective assessment of test reactions and the participants......-dependent results were obtained with Dermatophagoides pteronyssinus, grass and cat with a reproducibility rate of 0.69 to 0.81 in patients and 0.60-0.96 in controls. A unique finding was a significant positive correlation between a positive atopy patch test, allergen dose and increase in transepidermal water loss...... and erythema, while measurement of capacitance did not distinguish between positive and negative reactions. The results of the present study do not support the routine use of atopy patch tests in the evaluation of adult patients with atopic dermatitis....

  4. Resposta cutânea a alérgenos ambientais em indivíduos atendidos em serviço de pneumologia, Maringá, Estado do Paraná, Brasil = Cutaneous response to environmental allergens in patients attended in pulmonology service of Maringá, Paraná State, Brazil

    Directory of Open Access Journals (Sweden)

    Gustavo Falavigna Guilherme

    2012-01-01

    Full Text Available As alergias respiratórias, comumente as rinites e a asma, vêm se tornando cada vez mais frequentes. Considerando que no Noroeste do Paraná há poucos dados referentes ao assunto, propusemos a verificar os agentes causadores de alergias respiratórias e suas intercorrências em indivíduos atendidos em serviço privado de pneumologia do município de Maringá, Paraná. Estudo tipo observacional transversal, retrospectivo, realizado de janeiro/2004 a dezembro/2005. Os participantes foram avaliados clinicamente e realizado o teste de hipersensibilidade imediata, Punctura – Prick TestR. De cada indivíduo foram anotados os seguintes dados: idade, positividade ao teste, principais alérgenos reagentes, presença ou ausência de atopia, rinite ou asma. De 396 indivíduos, 236 (59,3% apresentaram positividade para um ou mais alérgenos, sendo que 80 (20,2% reagiram a três antígenos, 85 (21,5% a quatro ou mais. A reatividade dos indivíduos foi mais frequente à poeira doméstica (207, Dermatophagoides pteronyssimus (184, Dermatophagoides farinae (158 e Blomia tropicalis (95. Inclusive, poeira domiciliar, D. pteronyssimus e D. farinae ocorreram principalmente em indivíduos com moderada a severa atopia. Aproximadamente 70% dos indivíduos com idade entre seis a 20 anos apresentaram atopia. Considerando o elevado nível de atopia nos pacientes atendidos pelo serviço de pneumologia em Maringá, é imprescindível realizar programas de controle no meio ambiente, juntamente com o acompanhamento e o tratamento médico dos indivíduos atópicos.Respiratory allergies, especially rhinitis and asthma, are becoming increasingly common. Given that in northwestern Paraná there is little data on the subject, our objective was to verify the causative agents of respiratory allergies and their complications in patients treated at a private pulmonology service in the municipality of Maringá, Paraná. The study was observational, retrospective, lasting from

  5. Resposta cutânea a alérgenos ambientais em indivíduos atendidos em serviço de pneumologia, Maringá, Estado do Paraná, Brasil - doi: 10.4025/actascihealthsci.v34i1.7920 Cutaneous response to environmental allergens in patients attended in pulmonology service of Maringá, Paraná State, Brazil - doi: 10.4025/actascihealthsci.v34i1.7920

    Directory of Open Access Journals (Sweden)

    Valentim Constante Sella

    2011-12-01

    Full Text Available As alergias respiratórias, comumente as rinites e a asma, vêm se tornando cada vez mais frequentes. Considerando que no Noroeste do Paraná há poucos dados referentes ao assunto, propusemos a verificar os agentes causadores de alergias respiratórias e suas intercorrências em indivíduos atendidos em serviço privado de pneumologia do município de Maringá, Paraná. Estudo tipo observacional transversal, retrospectivo, realizado de janeiro/2004 a dezembro/2005. Os participantes foram avaliados clinicamente e realizado o teste de hipersensibilidade imediata, Punctura – Prick TestR. De cada indivíduo foram anotados os seguintes dados: idade, positividade ao teste, principais alérgenos reagentes, presença ou ausência de atopia, rinite ou asma. De 396 indivíduos, 236 (59,3% apresentaram positividade para um ou mais alérgenos, sendo que 80 (20,2% reagiram a três antígenos, 85 (21,5% a quatro ou mais. A reatividade dos indivíduos foi mais frequente à poeira doméstica (207, Dermatophagoides pteronyssimus (184, Dermatophagoides farinae (158 e Blomia tropicalis (95. Inclusive, poeira domiciliar, D. pteronyssimus e D. farinae ocorreram principalmente em indivíduos com moderada a severa atopia. Aproximadamente 70% dos indivíduos com idade entre seis a 20 anos apresentaram atopia. Considerando o elevado nível de atopia nos pacientes atendidos pelo serviço de pneumologia em Maringá, é imprescindível realizar programas de controle no meio ambiente, juntamente com o acompanhamento e o tratamento médico dos indivíduos atópicos.Respiratory allergies, especially rhinitis and asthma, are becoming increasingly common. Given that in northwestern Paraná there is little data on the subject, our objective was to verify the causative agents of respiratory allergies and their complications in patients treated at a private pulmonology service in the municipality of Maringá, Paraná. The study was observational, retrospective, lasting from

  6. Clinical course of cow's milk protein allergy/intolerance and atopic diseases in childhood

    DEFF Research Database (Denmark)

    Høst, Arne; Halken, Susanne; Jacobsen, Hans

    2002-01-01

    ; and thereafter every 12 months until the age of 15 years. From the same birth cohort, 276 infants were randomly selected at birth for prospective non-interventional follow-up in order to investigate the natural course of sensitization and development of atopic disease during childhood. Standardized...... prick test and specific sIgE (Pharmacia CAP) testing were performed at 18 months, 5, 10 and 15 years of age against a panel of inhalant allergens (birch, grass, mugwort, dog, cat, horse, Dermatophagoidespteronyssinus, Dermatophagoides farinae, alternaria and cladosporium herbarum). Furthermore, lung...... prognosis, whereas children with IgE-mediated CMPA in early childhood had a significantly increased risk for persistent CMPA, development of other food allergies, asthma and rhinoconjunctivitis. During early infancy, recurrent wheezing was the most prevalent disease (20%), followed by atopic dermatitis (14...

  7. A clinic study on desensitization treatment of bronchial asthma with positive specific IgE to dust mite in children%尘螨特异性IgE阳性儿童支气管哮喘脱敏治疗临床研究

    Institute of Scientific and Technical Information of China (English)

    蔡幸生; 朱勇斌; 林丽爱; 黄育涛; 陈素华; 王金燕; 林彤彤

    2016-01-01

    Objective To investigate the efficacy and the course of desensitization treatment in bronchial asthma with positive specific IgE to dust mite in children. Methods A total of 105 children with bronchial asthma with positive specific IgE to dermatophagoides farinae allergens were randomized into the observation group and the control group. Children in the control group were treated to continue anti-asthma according to the routine of prevention and treatment children with asthma. Chinldren in the observation group were treated by dermatophagoides farinae drops in addition to the treatment of children in the control group. The recurrence of asthma was compared between two groups at 25 weeks post-treatment. At 25 weeks post-treatment , children in the observation group was randomly divided into the observation groupⅠand group Ⅱ. Children in the observation groupⅠreceived continuous treatment except for desensitization treatment. Children in the observation group II received the sublingual immunotherapy with dermatophagoides farinae drops (No.4) for 1 year in addition to the treatment in the observation groupⅠ. The recurrence of asthma was also compared between the two sub-groups. Results The rate and times of recurrence of asthma were lower in the observation group than those in the control group(P 0.05). Conclusion The recurrent rate and frequency of asthma could be reduced by the sublingual immunotherapy with dermatophagoides farinae drops in children with asthma of positive specific IgE to dust mite. The course of treatment may be half year long.%目的:探讨尘螨特异性IgE阳性儿童支气管哮喘脱敏治疗的临床疗效及疗程。方法:105例血清粉尘螨抗原阳性的支气管哮喘患儿在临床缓解后被随机分成观察组和对照组,对照组继续按哮喘诊疗常规进行治疗,观察组在对照组治疗基础上,予粉尘螨滴剂1~4号舌下含服脱敏治疗,治疗满25周时,比较两组哮喘复发情况。25

  8. Dicty_cDB: Contig-U12146-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available TCC 354... 86 1e-15 CP000614_2346( CP000614 |pid:none) Burkholderia vietnamiensis G4 c... 86 2e-15 CP000058_24( CP000058 |pid...) Methylobacterium extorquens AM1... 42 0.031 CP000615_1842( CP000615 |pid:none) Burkholderia vietnam...:none) Azotobacter vinelandii DJ, comple... 84 5e-15 FM209186_23( FM209186 |pid:none) Pseudomonas aerugin...hromobacterium violaceum ATCC ... 40 0.15 AL939117_280( AL939117 |pid:none) Streptomyces coelicolor A3(2) co...B284897 ) DF1717 Dermatophagoides farinae cDNA library Derm... 46 2.4 1 ( FF990576 ) CBWU108028.b1 Yutaka Satou unpublished cDNA li

  9. Chemical Composition and Inhibitory Effect of Lentinula edodes Ethanolic Extract on Experimentally Induced Atopic Dermatitis in Vitro and in Vivo

    Directory of Open Access Journals (Sweden)

    Eun-Ju Choi

    2016-07-01

    Full Text Available The ethanolic extract of Lentinula edodes was partially analyzed and then characterized for its efficacy in treating atopic dermatitis. Polyphenols were determined to be the major antioxidant component in the extract (6.12 mg/g, followed by flavonoids (1.76 mg/g, β-carotene (28.75 μg/g, and lycopene (5.25 μg/g. An atopic dermatitis (AD model was established and epidermal and dermal ear thickness, mast cell infiltration, and serum immunoglobulin levels were measured after oral administration of the L. edodes extract for 4 weeks. L. edodes extract decreased Dermatophagoides farinae extract (DFE and 4-dinitrochlorobenzene (DNCB-induced expression of several inflammatory cytokines in the ears, cervical lymph nodes, and splenocytes. Consequently, L. edodes extract may have therapeutic potential in the treatment of AD attributable to its immunomodulatory effects.

  10. The correlation between intradermal testing and serum specific IgE to house dust mite in negative skin prick test allergic rhinitis adult patients.

    Science.gov (United States)

    Tantilipikorn, Pongsakorn; Danpornprasert, Piyanart; Ngaotepprutaram, Premyot; Assanasen, Paraya; Bunnag, Chaweewan; Thinkhamrop, Bandit

    2015-12-01

    Diagnosis of allergic rhinitis (AR) is based on history, physical examination, and skin prick test (SPT) while intradermal (ID) test can be performed to confirm the diagnosis in case of negative result of SPT. However, the ID test is not recommended for cat and timothy grass allergy because of its high false positive rate. As a result, the "quantitative" technique of serum specific IgE (sIgE) measurement might be helpful to diagnose AR with more confidence. To evaluate the correlation between ID tests and sIgE in the diagnosis of house dust mite (HDM)-sensitive AR patients. Patients with chronic rhinitis (CR) were recruited and SPT was performed. If SPT was negative, ID test and sIgE to HDM [Dermatophagoides pteronyssinus (Dp)] measurement were performed. Eighty-two patients with chronic rhinitis (CR), whose SPTs were negative for Dp, were included. There were 39 males (47.6%) and 43 females (52.4%) aged between 18 and 76 years old (mean age = 43.3 years). The ID test was positive in 13 patients (15.9%), and was negative in 69 patients (84.1 %). sIgE to HDM was positive ( ≥ 0.35 kUA/l) in 2 patients (2.4%). There was a fair to moderate correlation between the size of wheal of ID test and sIgE to HDM (r = 0.44, 95% confidence interval: 0.19 to 0.67, p correlation with sIgE Dermatophagoides pteronyssinus and it can be used in CR patients with negative SPT where sIgE is not feasible.

  11. Th2 polarization by Der p 1--pulsed monocyte-derived dendritic cells is due to the allergic status of the donors.

    Science.gov (United States)

    Hammad, H; Charbonnier, A S; Duez, C; Jacquet, A; Stewart, G A; Tonnel, A B; Pestel, J

    2001-08-15

    The polarization of the immune response toward a Th2 or a Th1 profile can be mediated by dendritic cells (DCs) following antigen presentation and interaction with T cells. Costimulatory molecules such as CD80 and CD86 expressed by DCs, the polarizing cytokine environment during DC--T-cell interaction, and also the nature of the antigen are critical in the orientation of the immune response. In this study, the effect of the cysteine protease Der p 1, one of the major allergens of the house dust mite Dermatophagoides pteronyssinus, on these different parameters was evaluated comparatively on monocyte-derived DCs obtained from healthy donors, from pollen-sensitive patients, or from patients sensitive to Dermatophagoides pteronyssinus. Results showed that Der p 1 induced an increase in CD86 expression only on DCs from house dust mite--sensitive patients. This was also associated with a higher capacity to induce T-cell proliferation, a rapid increase in the production of proinflammatory cytokines, tumor necrosis factor--alpha and interleukin (IL)-1 beta, and the type 2 cytokine IL-10. No changes in the release of IL-12 p70 were induced by Der p 1. Finally, purified T cells from house dust mite-sensitive patients stimulated by autologous Der p 1--pulsed DCs preferentially produced IL-4 rather than interferon-gamma. These effects were abolished in the presence of the inactive precursor of Der p 1 (ProDer p 1). Taken together, these data suggest that DCs from house dust mite--sensitive patients, in contrast to DCs from healthy donors and from pollen-sensitive patients, exposed to Der p 1 play a pivotal role in the enhancement of the Th2 response associated with the allergic reaction developed in response to house dust mite exposure. (Blood. 2001;98:1135-1141)

  12. Importancia médica de los alergenos de ácaros domésticos

    Directory of Open Access Journals (Sweden)

    Liliana Moreno

    1995-06-01

    Full Text Available En la actualidad, los trastornos respiratorios están entre los problemas de salud más frecuentes y, entre ellos, el asma aparece como una entidad muy común en niños y adultos. El asma alérgica es una enfermedad multifactorial. El componente ambiental es de gran importancia, lo cual ha sido ampliamente documentado gracias a los avances científicos que en los últimos años se han dado en el campo de la alergología experimental. Los ácaros domésticos son la principal fuente de alergenos en el polvo casero; dichos alergenos son considerados, hoy en día, como los principales inductores de las manifestaciones alérgicas respiratorias. Estos animales son artrópodos de distribución mundial, siendo Dermatophagoides pteronyssinus y Blomia tropicalis los que más frecuentemente se encuentran en el ambiente doméstico. Las mencionadas especies ejercen un gran impacto clínico porque la mayoría de la población asmática y rinítica presenta altos niveles de IgE contra sus alergenos lo cual, sin duda, influye en la patogénesis de la inflamación crónica que caracteriza a esas enfermedades. La presente revisión describe la biología, epidemiología y aspectos moleculares de los alergenos de Dermatophagoides pteronyssinus y Blomia tropicalis, como una información muy útil para los médicos y demás integrantes del equipo de salud que tengan el interés y la oportunidad de efectuar un manejo integral de estos problemas.

  13. [Screening of specific IgE-binding epitopes of dust mite allergens using short peptide array].

    Science.gov (United States)

    Teng, Feixiang; Sun, Jinxia; Wang, Nan; Yu, Lili; Cui, Yubao

    2017-08-01

    Objective To screen the possible linear epitopes of major and mid-potency allergens in Dermatophagoides farinae (Der f1, Der f2, Der f4, Der f5 and Der f7). Methods Short peptides were synthesized on the basis of the amino acid sequences in active fraction of Der f1, Der f2, Der f4, Der f5 and Der f7. Each peptide had eight amino acids in length and seven of them were overlapped with each other. Put these peptides to the chip to build microarrays that would have immunoreaction with human serum IgE. Then the chips were scanned to analyze the data. Results A total of 1128 short peptides from the above five groups of allergens were synthesized, and the microarray chips were constructed. Six serum samples from children who were allergic to Dermatophagoides farinae were mixed and added to the microarray chips. The chips were scanned and analyzed, and the results showed that Der f1 had four epitopes (46-53aa, 71-78aa, 99-110aa and 179-186aa), that Der f2 had three epitopes (15-22aa, 80-89aa and 106-113aa), that Der f 4 had six epitopes (69-82aa, 107-116aa, 225-232aa, 261-268aa, 355-365aa and 483-496aa), that Der f5 had one epitope (102-109aa), and Der f7 had three epitopes (32-39aa, 52-64aa and 100-107aa). Conclusion We identified the linear epitopes of Der f1, Der f2, Der f4, Der f5 and Der f7.

  14. Long-term falls in antibodies to dust mite and pollen allergens in patients with asthma or hay fever.

    Science.gov (United States)

    Rawle, F C; Burr, M L; Platts-Mills, T A

    1983-09-01

    'Spontaneous' improvement in, or alteration of, allergic symptoms is a common occurrence, and the immunological basis is of interest in attempts to develop effective specific therapy. In the present study we measured levels of serum antibodies to Dermatophagoides pteronyssinus in patients diagnosed as having house-dust-allergic asthma up to 40 years previously. The results show a progressive fall in both IgG and IgE antibodies to antigen P1 and RAST binding to crude D. pteronyssinus extract. By contrast changes in total serum IgE were not marked. Within each of the groups of patients diagnosed 20, 30 and 40 years previously, 70% no longer suffered severe symptoms. However, the absence of detectable IgE antibody in serum was neither a necessary nor a sufficient condition for loss of symptoms. A group of patients who had spontaneously recovered from hay fever had significantly lower IgG and IgE antibody for the major grass pollen allergen Rye I and also lower total IgE than current hay fever sufferers. In neither hay fever nor asthma was there evidence to link spontaneous improvement in symptoms with an increase in IgG antibodies.

  15. 487 Safety of Sublingual Immunotherapy with Standardized Vaccines of Domestic Mites

    Science.gov (United States)

    Alvarez, Mirta; Castro, Raúl; Gutierrez, Daniel; Labrada, Alexis; Enriquez, Irene; Ronquillo, Mercedes; Rodríguez, José; García, Iris

    2012-01-01

    Background Allergen-specific immunotherapy consists of administering gradually increasing doses of the allergen, to which the patient is sensitized, aiming at achieving tolerance to it and decreasing clinical symptoms. The sublingual immunotherapy (SLIT) was introduced as an alternative to subcutaneous route. Its use is being increased in the world and in Cuba, using standardized vaccines owing to greater safety. The objective of this study was to determine the safety of sublingual standardized vaccines of 3 domestic mite species (Valergen, Cuba) and its adverse events in allergic patients from the Calixto García University Hospital in Havana, as well as the frequency of its prescription. Methods Descriptive and cross sectional study design, which included 130 patients with treatment of SLIT with VALERGEN-DP (Dermatophagoides pteronyssinus), VALERGEN-DS (D. siboney) and VALERGEN-BT (Blomia tropicalis) (BIOCEN, Cuba), who attended the Allergy Service in the period January-September 2010. Age distribution: mean 19.6 years (range 1–75), 40.7 % was younger than 18 years. Results The multiallergen vaccine was the type of vaccine most used (63.8%). The most common allergen was D. pteronyssinus followed by B. tropicalis. 71.55% of administered allergens vaccines were in maintenance phase. We found 4 adverse events (3.1% of patients), all local, mild, and not requiring treatment or change of vaccination dosing schedule. Conclusions The Valergen vaccines by sublingual route are safe and well tolerated in Cuban allergic patients.

  16. Barnacle allergy: allergen characterization and cross-reactivity with mites.

    Science.gov (United States)

    Marinho, S; Morais-Almeida, M; Gaspar, A; Santa-Marta, C; Pires, G; Postigo, I; Guisantes, J; Martínez, J; Rosado-Pinto, J

    2006-01-01

    Barnacles are a type of seafood with worldwide distribution and abundant along the shores of temperate seas. They are particularly appreciated and regularly consumed in Portugal as well as in Spain, France and South America, but barnacle allergy is a rare condition of which there is only one reference in the indexed literature. The molecular allergens and possible cross-reactivity phenomena implicated (namely with mites) have not been established. To demonstrate the IgE-mediated allergy to barnacle and to identify the proteins implicated as well as possible cross-reactivity phenomena with mites. We report the clinical and laboratory data of five patients with documented IgE-mediated allergy to barnacle. The diagnosis was based on a suggestive clinical history combined with positive skin prick tests (SPT) to barnacle--prick to prick method. Two barnacle extracts were prepared (raw and cooked barnacle) and sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE) and IgE-immunoblotting were performed. An immunoblotting inhibition assay with Dermatophagoides pteronyssinus was also done in order to evaluate cross-reactivity. All patients had mite-related asthma and the allergic rhinoconjunctivitis; they all experienced mucocutaneous symptoms. All of them had positive SPT to barnacle, and the immunoblotting showed several allergenic fractions with a wide molecular weight range (19 - 94 kDa). The D. pteronyssinus extract inhibited several IgE-binding protein fractions in the barnacle extract. We describe five patients with IgE-mediated barnacle allergy. We also describe a group of IgE-binding+proteins between 30 and 75 kDa as the allergenic fractions of this type of Crustacea. Cross-reactivity with D. pteronyssinus was demonstrated in two cases.

  17. A Six-SNP Haplotype of ADAM33 Is Associated with Asthma in a Population of Cartagena, Colombia

    Science.gov (United States)

    Vergara, Candelaria I.; Acevedo, Nathalie; Jiménez, Silvia; Martínez, Beatriz; Mercado, Dilia; Gusmão, Leonor; Barnes, Kathleen C.; Caraballo, Luis

    2010-01-01

    Background A disintegrin and metalloprotein-33 (ADAM33) participates in the bronchial remodeling process in asthma, and genetic analyses pointed it out as a candidate gene in asthma. Methods To analyze the association between ADAM33 and asthma and total and mite-specific IgE levels in a population of the Caribbean Coast of Colombia, we genotyped 6 single-nucleotide polymorphisms of ADAM33 in 429 asthmatics, 401 controls and 116 family trios using fluorogenic probes. Total and specific IgE against Blomia tropicalis and Dermatophagoides pteronyssinus were determined by ELISA. Case-control and family-based analyses were performed. Case-control association analyses were corrected by population stratification using a set of 52 ancestry-informative markers. Results Eight common haplotypes were identified; among them, H4 (GCAGGG) was associated with asthma in the family group (Z score: −2.049, p = 0.04). We also found an association between the TT genotype of ST+7 and asthma in the case-control study (p = 0.05) that disappeared after correcting for multiple testing. In the family-based analysis, this genotype was a risk factor for asthma (p = 0.01), high total IgE (Z score: 2.546, p = 0.01) and high specific IgE against B. tropicalis (p = 0.02) and D. pteronyssinus (Z score: 2.414, p = 0.01). V4 was associated with specific IgE against B. tropicalis (p = 0.03); T2 with asthma (p = 0.03), high total IgE (p = 0.02) and IgE against D. pteronyssinus (p = 0.03) and T1 with high total IgE (p = 0.04). None of these associations was maintained after correction for multiple testing. Conclusions Our findings suggest a relevant role of ADAM33 in thepathogenesis of asthma in this population. PMID:19940503

  18. Angelicae Dahuricae Radix Inhibits Dust Mite Extract-Induced Atopic Dermatitis-Like Skin Lesions in NC/Nga Mice

    Directory of Open Access Journals (Sweden)

    Hoyoung Lee

    2012-01-01

    Full Text Available We examined whether Angelicae Dahuricae Radix (AR suppresses the development of atopic dermatitis (AD-like skin lesions induced by Dermatophagoides farinae in NC/Nga mice. To investigate the effect of AR, we measured the AD severity score, measured plasma levels of IgE and histamine, and performed histological analysis in NC/Nga mice. We also confirmed the anti-inflammatory effects of AR by measuring TARC/CCL17 production from LPS-treated RAW 264.7 cells and mRNA levels of TARC and MDC/CCL22 in TNF-α/IFN-γ-treated HaCaT cells. 10 mg/day of AR extract was applied for 4 weeks to NC/Nga mice. Both the AR extract and 0.1% tacrolimus suppressed the development of AD-like skin lesions and reduced dermatitis scores of the back and ear skin. AR extracts caused an inhibition of histological changes induced by repeated application of D. farinae and a reduction of IgE and histamine levels in plasma (P<0.05. Furthermore, NO production in LPS-treated RAW 264.7 cells was diminished in a dose-dependent manner, and hTARC production and TARC and MDC mRNA levels in TNF-α/IFN-γ-treated HaCaT cells were diminished by AR. The inhibitory effect of AR on NO, TARC and MDC production may be associated with the suppression of AD-like skin lesions in D. farinae-induced NC/Nga mice.

  19. Participação da sensibilidade atópica em pacientes com ceratoconjuntivite alérgica primaveril Participation of atopic sensitivity in patients with vernal allergic keratoconjunctivitis

    Directory of Open Access Journals (Sweden)

    Denise Atique Goulart

    2004-06-01

    Full Text Available OBJETIVOS: Estudar as respostas dos testes de punctura cutâneos nos pacientes portadores de ceratoconjuntivite vernal e comparar as respostas com as da população alérgica sem acometimento ocular. MÉTODOS: Foram realizados testes alérgicos de punctura em 48 pacientes portadores de ceratoconjuntivite alérgica primaveril, sendo 10 da forma limbar, 19 da forma palpebral e 19 da forma mista. O grupo controle foi formado por 52 pacientes do ambulatório de alergia da Santa Casa de São Paulo, portadores de alergia sistêmica, porém sem acometimento ocular. Testes cutâneos de punctura foram realizados em 48 pacientes do Setor de Córnea e Doenças Externas Oculares, do Ambulatório de Alergia Ocular do Departamento de Oftalmologia da Santa Casa de São Paulo, no período de julho de 2001 a setembro de 2002. O indicativo de resposta alérgica positiva era reação cutânea maior que 2 mm ocorrendo até 15 minutos da aplicação do alérgeno. Foram avaliadas as respostas aos seguintes alérgenos: pó doméstico, Dermatophagoides pteronissynus, Dermatophagoides farinae, Tyrophagus putrescentiae, Blomia tropicalis, Rhizopus spp, Penicillium, Alternaria alternata e pólens de gramíneas, disponíveis naquele serviço no dado período. RESULTADOS: Na população estudada, o sexo masculino foi mais freqüente, com 32 pacientes (66,6% no grupo de estudo e 27 no controle (51,9%. A idade média foi 10,0 ± 4,7 anos no grupo de estudo e 6,83 ± 3,6 anos no grupo controle. O alérgeno mais comumente causador de crises nos pacientes foi Dermatophagoides pteronissynus, com positividade em 26 pacientes, seguido por Dermatophagoides farinae, com 24 pacientes, Blomia tropicalis com 22, pó doméstico com 17, T. putrescentiae com 15, e Rhizopus com 3. Os alérgenos Penicillium, Alternaria alternata, e pólen de gramíneas tiveram cada um apenas 1 paciente com positividade no grupo com CCP. CONCLUSÕES: A semelhança do resultado do teste nos pacientes sem

  20. Sensibilización a ácaros domésticos en niños asmáticos severos

    Directory of Open Access Journals (Sweden)

    Adalfa Chang Gómez

    Full Text Available Introducción: las enfermedades alérgicas y el asma incrementan su prevalencia en Cuba y a nivel mundial. Los ácaros del polvo se encuentran entre los alérgenos perennes más prevalentes en todo el mundo. Objetivo: determinar la sensibilización a 3 especies de ácaros domésticos en los niños asmáticos severos de la Escuela Especial "Celia Sánchez Manduley", de Tarará, provincia La Habana. Métodos: se realizó un estudio descriptivo transversal en 91 alumnos, durante el curso escolar 2011-2012, y a toda la muestra se le realizaron pruebas cutáneas por punción (prick test, utilizando extractos Vallergen-BT (Blomia tropicalis, Vallergen-DS (Dermatophagoides siboney y Vallergen-DP (Dermatophagoides pteronyssinus confeccionados por el Centro de Biopreparados, en Cuba; además se determinó IgE sérica total. Resultados: la rinitis alérgica resultó la comorbilidad alérgica más frecuente. El total de los pacientes presentó reactividad cutánea positiva a los ácaros, así como IgE sérica total elevada. La sensibilización frente al D. pteronyssinus se reportó en el 93,4 % de los pacientes. No existió diferencia estadísticamente significativa en el diámetro del habón. Existió correlación entre la positividad de la IgE sérica total y la sensibilización cutánea a los 3 ácaros del polvo estudiado. Conclusiones: existe una estrecha relación entre el asma bronquial y la sensibilización a ácaros, con predominio de la especie D. Pteronyssinus.

  1. 海南儿童哮喘常见吸入性变应原的调查%Survey of common inhaled allergens on asthmatic children in Hainan

    Institute of Scientific and Technical Information of China (English)

    陈实; 王灵; 陈冰; 林小珍; 陈晓玲

    2011-01-01

    Objective To investigate the prevalenve of common inhaled allergens in asthmatic children in Hainan for exploring an effective management of allergen avoidance and providing a basis in the promotion of specific immunotherapy. Methods The skin prick test (SPT) with a panel of 13 allergen extracts was undertaken in 2 361 children with asthma, and 450 cases of them futher received specific IgE test against 10 allergens. Results The results of the SPT showed that 86% of the patients were sensitized to at least one allergen extract, and consisting of 91.2% for Dermatophagoides pteronyssinus (Der p), 89.3% for Dermatophogoides farinae (Der f) and 86.3% for Blomia tropicalis (Blo t), respectively. The pereentage of strong SPT positive eases (> 3 class) against three kinds of mites was 65.7% for Der p, 65.4% for Der f and 58.2% for Blo t, respectively. Furthermore, the SPT positive rates for animal dander in children with asthma were 22.4% for dog dander, 14.4% for cat dander, 28.7% for German cockroach, and 21.9% for America cockroach, respectively. However, the positive levels to these alergens were generally low. The positive rate of the specific IgE test in 450 children with asthma was 72.4%, and consisting of 66.2% for Der p and 59.6% for Der f. Conclusions There is a very high prevalence of the sensitization against Der p, Der f, and Blo t,and the sensitization against animal dander including dog, cat and cockroach is also found in a certain proportion in Hainan, that will be significant to avoid allergens and to promote the specific immunotherapy in asthmatic children in Hainan.%目的 了解海南地区儿童哮喘常见吸入性变应原,探讨有效实施变应原避免的措施,为成功开展特异性免疫治疗提供依据.方法 对2 361例哮喘儿童进行13种常见过敏原皮肤点刺试验,对其中450例患儿进行10种过敏原体外特异性IgE检测.结果 2 361例哮喘患儿皮肤点刺阳性率为86.0%,其中,屋

  2. 血清霉菌特异性IgE阳性支气管哮喘患者的临床及过敏状态分析%Analysis of clinical features and allergic status of asthmatic patients with positive serum mycosisspecific IgE

    Institute of Scientific and Technical Information of China (English)

    牟艳; 叶伶; 龚颖; 张志风; 金美玲

    2013-01-01

    Objective To improve understanding of the clinical characteristics and diagnosis of allergic bronchopulmonary mycosis (ABPM).Methods We retrospectively analyzed the clinical data,including clinical symptoms,laboratory tests,pulmonary function tests and chest CT imaging of 95 asthmatic patients with positive serum mycosis-specific IgE from January 2010 to September 2012 in Zhongshan Hospital Affiliated to Fudan University.Results Of the 95 patients,59 cases met the diagnostic criteria of ABPM.There were 34 males and 25 females,with a mean age of (53 ± 4) years and a duration of asthma for (21 ±4) years.Thirty-six cases showed mycosis hypersensitivity (MH).There were 10 males and 26females,with a mean age of (46 ± 6) years and a duration of asthma for (16 ± 5) years.Clinical symptoms such as wheeze (52 vs 21,x2 =11.159,P =0.001),cough (54 vs 27,x2 =4.859,P =0.030) and expectoration (43 vs 9,x2 =25.731,P =0.000) were more common in the ABPM group compared to the MH group.In the ABPM group,58 were A.fumigatus-specific IgE antibody positive,34 Penicillium-specific IgE antibody positive and 1 only Penicillium-specific IgE antibody positive.While in the MH group,15 were A.fumigatus-specific IgE antibody positive,24 Penicillium-specific IgE antibody positive and 17 only Penicillium-specific IgE antibody positive.In the ABPM group,the percentage of positive fumigatus-specific IgE antibody was higher (58 vs 15,x2 =24.500,P =0.000),while the percentages of dermatophagoides pteronyssinus (21 vs 20,x2 =3.632,P =0.045) and Dermatophagoides farinae (17 vs 21,x2 =8.118,P =0.004) were lower.Total serum IgE [(4395 ± 1437) IU/ml vs (276 ± 133) IU/ml,T =4.384,P =0.000],peripheral eosinophil percentage [(12.56 ± 1.20) % vs (1.30 ± 0.15) %,t =8.175,P =0.000]and count [(2.09 ±0.43) × 109/L vs (0.19 ±0.04) × 109/L,t =7.032,P =0.000] were higher in the ABPM group as compared to the MH group.FEV1 % slightly declined in the ABPM group [(70.2 ± 2.3)%vs (78.3 ±3.2

  3. Perfil de consulta en niños alérgicos provenientes de familias de bajos ingresos Profile of consultation of allergic children from low income families

    Directory of Open Access Journals (Sweden)

    Alain Raimundo Rodríguez-Orozco

    2007-09-01

    Full Text Available Las enfermedades alérgicas son una de las principales causas de atención médica en la infancia y su impacto se acentúa más en las familias de bajos ingresos. En un estudio descriptivo analítico se caracterizó el perfil de consulta del niño alérgico proveniente de familias mexicanas de bajos recursos económicos. Las enfermedades alérgicas predominaron en el sexo masculino y la edad escolar; el 71 % de los enfermos provenía de localidades urbanas. El asma fue el diagnóstico más frecuente (64 %, seguido de la rinitis alérgica (30 %, dermatitis atópica (6 % y urticaria (3 %. Las reactividades encontradas con más frecuencia en la prueba cutánea fueron Dermatofagoides farinae (77 %, Dermatofagoides pteronyssinus (60 %, Phleum pratense (20 %, gato (17 %, perro (14 % y Cynodon dactylon (11 %. El alto grado de disfunción familiar y la poca adhesión a tratamientos prolongados posibilitan la perpetuidad de los síntomas y el pronóstico incierto en este grupo de niños.Allergic diseases are one of the main causes for seeing the doctor in childhood and their impact is more acute in low income families. An analytical descriptive study characterized the profile of medical consultation of the allergic child from Mexican low income families. Allergic diseases prevailed in males and at school age, and 71 % of the sick children lived in urban settings. Asthma was the most frequent diagnosis (64 % followed by allergic rhinitis (30%, atopic dermatitis (6 % and urticaria (3 %. The most commom reactivity rates in the cutaneous test were Dermatofagoides farinae (77 %, Dermatofagoides pteronyssinus (60 %, Phleum pratense (20 %, cat (17 %, dog (14 % and Cynodon dactylon (11 %. The high level of family dysfunction and low adhesion to long therapies make it possible the persistence of symptoms and the uncertain prognosis in this group of children.

  4. Measurement and analysis of serum specific IgE and total IgE in children with chronic eczema%364例儿童慢性湿疹血清特异性IgE和总IgE检测分析

    Institute of Scientific and Technical Information of China (English)

    姜日花; 朱明姬; 贾玉玺; 蒋忠民

    2009-01-01

    目的 了解慢性湿疹患儿血清特异性IgE和总IgE水平,为预防和治疗该病提供理论依据.方法 采用德国Mediwiss 过敏原体外检测系统,应用免疫印迹定量法对364例慢性湿疹患儿进行血清特异性IgE和总IgE检测.结果 慢性湿疹患儿总 IgE 阳性检出率为48.9%;吸入变应原中户尘螨粉尘螨、蟑螂、矮豚草蒿、狗猫皮屑、霉菌阳性检出率分别为 29.7%,14.3%,13.5%,12.6%,12.1%;食入变应原中蛋白蛋黄、牛奶、鱼虾蟹阳性检出率分别为 18.1%,15.9%,12.6%;1-3岁组食入性变应原的阳性率为高于6-12岁组(P<0.05),而吸入性变应原的阳性率则低于6-12岁组(P<0.05).结论 尘螨、蟑螂、矮豚草蒿、猫毛狗毛皮屑、霉菌、鸡蛋、牛奶、鱼虾蟹是吉林地区小儿慢性湿疹常见的变应原.小于3岁湿疹患儿主要对蛋白蛋黄等食入性变应原过敏,而大于6岁患儿主要对粉尘螨、蟑螂、矮豚草蒿等吸入性变应原过敏.%Objective To study the serum specific IgE (sIgE) and total IgE(tIgE) level in children with chronic eczema and to lie a theoretical foundation for its clinical prevention and treatment.Methods Quantitative immunoblot analysis (Allergy Mediwiss stemystem) was used to examine sIgE and tIgE level in 364 chronic eczema children.All subjects were tested for food-induced and airborne-induced allergens equally.Results The detecting rate of tIgE was 48.9%; while that of dermatophagoides farinae,cockroach,low rag weed,canine and feline scurf,mycetes was 29.7%,14.3%,13.5%,12.6%,12.1%,respectively in airborne-induced group,and of Milk,fish and shrimp,pea crab was 18.1%,15.9%,12.6%,respectively in food-induced group.It showed a significantly higher positive rate of food-induced allergens in toddlers (1-3 years old) than those in school-agers (6-12 years old) (P<0.05),whereas overturned in airborne-induced allergens dramatically(P<0.05).Conclusion Dermatophagoides farinae,cockroach,low rag weed,canine and

  5. 宜昌地区支气管哮喘患儿主要过敏原检测分析%Analysis of the common allergens for asthma children in Yichang

    Institute of Scientific and Technical Information of China (English)

    文丹丹; 王敏; 罗飞; 彭经纬; 韩蓓

    2013-01-01

    OBJECTIVE To determine the spectrum of common allergens tor children with asthma in Yichang,to provide evidences on prevention and treatment of allergic respiratory tract diseases in children.METHODS The skin prick test (SPT)was performed in 218 children who coming from childhood asthma specialist clinic in Yichang Central People's Hospital from Mar 2011 to Feb 2012.RESULTS (1) SPT was successfully performed in all 218 patients.The positive rate of SPT was 74.31% (162 case) ; (2) An allergen positive rate was 27.52% (60 cases),two or more kinds of allergens positive rate was 72.48% (158 cases) ; (3) In SPT positive patients,the allergens with highest positive percentages were house dust mite (71.10%),dermatophagoides farinae (70.64%),then dog hair (4.13%) and cat hair (3.67%) ; (4) There was a significant relation between allergen skin prick test results of house dust mite and dermatophagoides farinae and severity of acute exacerbation of asthma.CONCLUSION Comprehensive detection and avoiding contact with allergens is the effective control of asthma in children.Because of the significant relation between allergen skin prick test results of dermatophagoides farinae and house dust mite and severity of acute exacerbation of asthma,avoid exposure to mite allergen could become an important measure to prevent severe acute exacerbation of asthma.%目的 探讨宜昌地区支气管哮喘患儿主要过敏原分布特点及意义,为儿童变应性呼吸道疾病的防治提供理论依据.方法 采用变应原皮肤点刺诊断试剂盒对2011年3月~2012年2月在宜昌市中心人民医院儿童哮喘专家门诊诊治的218例哮喘患儿进行皮肤点刺试验.结果 (1)对218例哮喘患儿检测发现,总阳性率为74.31%(162例);(2)一种过敏原阳性率27.52% (60例),两种或两种以上种过敏原阳性率72.48%(158例);(3)最常见过敏原的前四位为户尘螨(71.10%)、粉尘螨(70.64%)、狗毛(4.13%)、猫毛(3.67%); (4

  6. Effectiveness of house dust mite acaricide tri-n-butyl tin maleate on carpets, fabrics and mattress foam: a standardization of methodology Eficácia do acaricida maleato de estanho tri-n-butílico contra ácaros de poeira em carpetes, tecidos e espuma de colchão: padronização de metodologia

    Directory of Open Access Journals (Sweden)

    Satiko Uehara

    2006-06-01

    Full Text Available The aim of this study was to determine the effectiveness of the acaricide tri-n-butyl tin maleate, industrially applied to samples of carpets, mattress foam, and fabrics used for furniture upholstery, soft toys and shoe uppers. Approximately 100 adult house dust mites of the species Dermatophagoides pteronyssinus were inoculated into a Petri dish containing the sample (a piece of carpet, mattress foam, or fabric, treated with the acaricide, randomly collected. Mite-maintenance culture medium was added on top of each sample. After one, two, three, seven and 30 days of incubation at 25 ºC and 75% relative humidity, each dish was examined using a 40X stereoscopic microscope (40X. One hundred percent acaricide effectiveness was obtained in treated materials by the end of the 30th-day postinoculation period, under optimal conditions for mite maintenance.O objetivo do presente estudo foi verificar a eficácia do acaricida maleato de estanho tri-n-butílico, aplicado industrialmente em amostras de carpetes, tecidos de revestimentos de móveis e de calçados, assim como de espumas de colchão. Aproximadamente 100 ácaros adultos da espécie Dermatophagoides pteronyssinus foram inoculados em placa de Petri contendo a amostra (pedaço de colchão, tecido ou carpete, tratada com o produto acaricida, coletados aleatoriamente. Foi acrescentado sobre a amostra, meio de cultivo para a manutenção dos ácaros. Cada placa foi examinada após 1, 2, 3, 7 e 30 dias de incubação a 25 ºC e 75% de U.R.A. (umidade relativa do ar, sob microscópio estereoscópico com 40X de aumento. O acaricida maleato de estanho tri-n-butílico apresentou 100% de eficácia acaricida após 30 dias da aplicação, em condições ótimas para a manutenção dos ácaros.

  7. An Exploratory Pilot Study of Genetic Marker for IgE-Mediated Allergic Diseases with Expressions of FcεR1α and Cε

    Directory of Open Access Journals (Sweden)

    En-Chih Liao

    2015-04-01

    Full Text Available The high affinity immunoglobulin E (IgE receptor-FcεR1 is mainly expressed on the surface of effector cells. Cross-linking of IgE Abs bound to FcεR1 by multi-valent antigens can induce the activation of these cells and the secretion of inflammatory mediators. Since FcεR1 plays a central role in the induction and maintenance of allergic responses, this study aimed to investigate the association of FcεR1 with the allergic phenotype of Cε expression and cytokine and histamine release from peripheral leukocytes. Peripheral leukocytes from 67 allergic and 50 non-allergic subjects were used for genotyping analysis. Peripheral mononuclear cells (PBMCs were used for Cε expression and ELISpot analysis, while polymorphonuclear cells (PMNs were used for histamine release. The association between genotype polymorphism of the FcεR1α promoter region (rs2427827 and rs2251746 and allergic features of Cε expression and histamine were analyzed, and their effects on leukocytes function were compared with wild type. The genotype polymorphisms of FcεR1α promoter region with CT and TT in rs2427827 and TC in rs2251746 were significantly higher in allergic patients than in non-allergic controls. Patients with single nucleotide polymorphism (SNP of FcεR1α promoter region had high levels of total IgE, mite-specific Der p 2 (Group 2 allergen of Dermatophagoides pteronyssinus-specific IgE and IgE secretion B cells. The mRNA expression of FcεR1α was significantly increased after Der p2 stimulation in PBMCs with SNPs of the FcεR1α promoter region. Despite the increased Cε mRNA expression in PBMCs and histamine release from PMNs and the up-regulated mRNA expression of interleukin (IL-6 and IL-8 secretions after Der p2 stimulation, there was no statistically significant difference between SNPs of the FcεR1α promoter region and the wild type. SNPs of FcεR1α promoter region were associated with IgE expression, IgE producing B cells, and increased Der p2

  8. Avaliação do teste de contato com aeroalérgenos em pacientes com dermatite atópica Evaluation of patch test with airbone allergic agents in patients with atopic dermatitis

    Directory of Open Access Journals (Sweden)

    Rosana Neves Dos Santos Rodrigues

    2011-02-01

    Full Text Available FUNDAMENTOS: a dermatite atópica é uma doença inflamatória cutânea que apresenta múltiplos fatores desencadeantes. Há vários relatos de autores que confirmaram os aeroalérgenos como fatores agravantes ou desencadeantes. O teste de contato com aeroalérgenos ou teste de contato atópico foi proposto para avaliar a participação destes alérgenos na dermatite atópica. OBJETIVO: objetivo deste estudo foi avaliar a positividade do teste de contato atópico em pacientes com dermatite atópica. MÉTODOS: Avaliamos 50 pacientes com dermatite atópica e 45 do grupo com rinite alérgica, nos quais realizamos teste de contato atópico com extratos de Dermatophagoides pteronissynus, Dermatophagoides farinae e Blomia tropicalis, além de testes cutâneos de leitura imediata para os mesmos alérgenos, acrescidos de epitélio de cão e gato e fungos. RESULTADOS: verificamos que o teste de contato atópico com ácaros apresentou maior positividade nos indivíduos do grupo de dermatite atópica quando comparado ao grupo de rinite alérgica. CONCLUSÕES: o teste de contato atópico apresenta resultados estatisticamente significativos quando realizado com ácaros, em pacientes com dermatite atópica, com p=0,035, OR (odds ratio = 3,35 e IC(95% = [ 1,18; 9,47].BACKGROUND: Atopic dermatitis is an inflammatory skin disease that can be triggered by many factors. Several reports confirm the role of airborne allergic agents as aggravating or triggering factors. The patch test with airborne allergic agents or the atopy patch test was suggested to evaluate the role of these allergens in atopic dermatitis. OBJECTIVE: This study aimed at evaluating the positivity of the atopy patch test in patients with atopic dermatitis. METHODS: We evaluated 50 patients with atopic dermatitis and 45 with allergic rhinitis, the atopy patch test was performed in these patiennts with extracts of Dermatophagoides pteronissynus, Dermatophagoides farinae and Blomia tropicalis, as

  9. Occupational exposure to allergenic mites in a Polish zoo.

    Science.gov (United States)

    Solarz, Krzysztof; Szilman, Piotr; Szilman, Ewa

    2004-01-01

    The study was carried out from April 2000-March 2001. During this period 49 samples of dust, litter, debris and residues from cages and run-offs of mammals, birds and reptiles in the Silesian Zoo, were examined for the presence of mites, especially the allergenic taxa. Mites were extracted using the Berlese method and preserved in 70 % ethanol. For identification, the mites were mounted in Hoyer's medium on microscope slides. Mites were found in 44 of 49 samples analyzed (89.8 %). A total of 5,097 mites were collected, from which 60.3 % were found in samples collected in spring, whereas only 13 % in summer and 24.1 % in autumn. The remaining 2.6 % of the total mite population was found in winter. Majority of mites (82.7 %) were collected from aviaries of macaws and cockatiels (Ara ararauna and Nymphicus hollandicus). A total of 10 species of astigmatid mites were identified that belong to 4 families--Acaridae, Glycyphagidae, Anoetidae and Pyroglyphidae. Generally, the allergenic mites of the order Astigmata constituted 49.5 % of the total count. Among them Acarus farris was predominant (34 % of the total count), followed by Tyrophagus putrescentiae (4.7 %), Caloglyphus sp. (4.35 %) and Acarus immobilis (4.31 %). Dermatophagoides farinae, the house-dust-mite species, was for the first time found in this environment. D. farinae (0.05 % of the total population) was associated with parrots, canids and artiodactyls. Summarizing, it should be stressed, that cages and run-offs of different mammals, aviaries of parrots and terrariums of snakes are important sources of some allergenic mites, especially A. farris and T. putrescentiae, that might cause allergies in workers.

  10. Prenatal Fish Oil Supplementation and Allergy: 6-Year Follow-up of a Randomized Controlled Trial.

    Science.gov (United States)

    Best, Karen P; Sullivan, Thomas; Palmer, Debra; Gold, Michael; Kennedy, Declan John; Martin, James; Makrides, Maria

    2016-06-01

    Evidence from randomized controlled trials in early infancy suggest that prenatal supplementation with Ω-3 (n-3) long-chain polyunsaturated fatty acids (LCPUFA) reduces the incidence of allergic disease characterized by an immunoglobulin E (IgE) response. We aimed to determine whether protective effects were evident in the 6-year-old offspring of women supplemented with n-3 rich fish oil during pregnancy. Six-year follow-up of children (n = 706) with a family history of allergic disease from the Docosahexaenoic Acid to Optimize Mother Infant Outcome (DOMInO) trial. Women were randomly allocated to receive n-3 LCPUFA-rich fish oil capsules (800 mg/d docosahexaenoic acid DHA and 100mg/d eicosapentaenoic acid) or vegetable oil capsules (without n-3 LCPUFA). Allergic disease symptoms including eczema, wheeze, rhinitis, and rhino-conjunctivitis, were assessed using the International Study of Asthma and Allergies in Childhood questionnaire and sensitization to allergens was measured by skin prick test. There was no difference in the percentage of children with any IgE-associated allergic disease between the n-3 LCPUFA and control groups (116/367 [31.5%] vs 106/336 [31.5%]; adjusted relative risk, 1.04; 95% confidence interval, 0.82-1.33; P = .73). There was a reduction in the percentage of children sensitized to house dust mite Dermatophagoides farinae (49/367 [13.4%] vs 68/336 [20.3%]; adjusted relative risk, 0.67, 95% confidence interval, 0.44-1.00; P = .0495). Prenatal n-3 LCPUFA supplementation did not reduce IgE-associated allergic disease at 6 years of age. Secondary outcomes were suggestive of a protective effect of the intervention on the incidence of D. farinae sensitization. Copyright © 2016 by the American Academy of Pediatrics.

  11. Analyzing of the inhaled allergens profiles of 890 allergic rhinitis patients%上海地区890例变应性鼻炎患者吸入变应原谱分析

    Institute of Scientific and Technical Information of China (English)

    丁俊杰; 张建华; 许芳; 徐颖; 朱华斌

    2012-01-01

    目的:了解上海地区变应性鼻炎患者吸入变应原分布情况,为该地区变应性鼻炎的流行病学研究和防治方案提供依据.方法:回顾分析890例门诊变应性鼻炎患者对13种吸入变应原进行皮肤点刺试验的临床资料,并比较不同年龄段及不同性别对吸入变应原阳性率的差异.结果:在上海地区变应性鼻炎患者中,最主要的吸入性变应原是屋尘螨(91.24%)、粉尘螨(86.58%)、热带螨(51.98%);其次是狗毛(15.96%),并且阳性率随年龄的增长有明显下降趋势,男女性别组间阳性率无明显差别.结论:屋尘螨、粉尘螨是上海地区变应性鼻炎最常见的变应原,变应原点刺试验有助于发现变应原,从而为脱敏治疗提供重要依据.%Objective:To investigate the inhaled allergens spectrum of 890 allergic rhinitis patients in Shanghai, and to provide basic epidemiologic information for the prevention and treatment of allergic rhinitis. Method: Thirteen allergens skin prick test results of 890 allergic rhinitis patients recruited were retrospectively analyzed. Result: The main common inhaled allergens in allergic rhinitis patients in Shanghai were house dust mite (91. 24%),dermatophagoides farinae(86. 58%) , tropical mite(51. 98%),dog hair(15. 96%). Moreover, the positive frequency was decreased with age increasing, and gender group had no obvious difference. Conclusioni House dust mite and dermatophagoides farinae were the main allergens of allergic rhinitis patients in Shanghai. Skin prick test is helpful for the discovery of the allergens so as to provide important basis of immunotherapy.

  12. Analyzing of the inhaled allergens profiles of 583 allergic rhinitis patients in district of QingPu%青浦地区变应性鼻炎患者吸入变应原检测分析

    Institute of Scientific and Technical Information of China (English)

    陈国辉; 白广平; 王丽英

    2012-01-01

    Objective To investigate the profiles of inhaled allergic rhinitis patients in district of Qingpu,and to provide basic epidetniologic information for the prevention and treatment of allergic rhinitis in this area.Methods Retrospectively analyze 14 allergens Skin prick test results of 583 allergic rhinitis patients recruited. Results the main common inhaled allergens in allergic rhinitis patients in QingPu were house dust mite(72.6%),dermatophagoides farinae(68.8%),tropical mite(41.3%),dog hair(12.7%),Moreover,the positive frequency was decreased with age increasing. Conclusion House dust mite and dermatophagoides farinae were the most common allergens in allergic rhinitis patients in QingPu area SPT is help&l for the discovery of the allergens so as to provide important basis of immunotherapy.%目的 了解青浦地区变应性鼻炎患者吸入变应原分布情况,为青浦地区变应性鼻炎的流行病学研究和防治提供重要依据.方法 回顾分析583例变应性鼻炎患者对14种吸入变应原进行皮肤点刺试验的临床资料,并比较两组年龄段及吸入变应原阳性率在分布上的差异.结果 在青浦地区变应性鼻炎患者中,最主要的吸入变应原是屋尘螨(72.6%)、粉尘螨(68.8%)、热带螨(41.3%),其次是狗毛(12.7%),并且阳性率随年龄的增长有明显下降趋势.结论屋尘螨、粉尘螨是青浦地区变应性鼻炎最常见的变应原.皮肤点刺试验(Skin Prick Test,SPT)有助于发现变应原,为免疫治疗提供重要依据.

  13. [Dust mites as occupational allergens in two bakeries of La Habana, Cuba].

    Science.gov (United States)

    Fernández-Duro, Bárbara I; Alvarez-Castelló, Mirta; Mateo-Morejón, Mayteé; Luis-Rodríguez, Bárbara; Labrada-Rosado, Alexis

    2014-01-01

    Antecedentes: las alergias ocupacionales son cada vez más frecuentes. Los alergenos del ambiente laboral de panaderías, como los ácaros, no están bien identificados a pesar de ser factores de riesgo conocidos de enfermedades respiratorias. Objetivos: aislar e identificarlas especies de ácaros en dos panaderías de La Habana, Cuba, con alta sensibilización a ácaros en sus trabajadores. Material y método: estudio descriptivo en el que se tomaron muestras por triplicado de las posibles fuentes de ácaros en las panaderías –como harina de trigo en uso, derrames de harina de trigo en los pisos, harina de trigo almacenada en sacos sellados, azúcar, levadura panadera granulada– que fueron procesadas, según el método de flotación de Hart and Fain (1987) para aislar, identificar y contar los ácaros presentes. Resultados: se encontraron ácaros en las muestras de harina de trigo en uso, en los derrames de ésta en los pisos y en la harina de trigo almacenada en sacos sellados. Las especies identificadas fueron Blomia tropicalis (70%), Tyrophagus putrescentiae (20%) y en mucho menor grado Dermatophagoides pteronyssinus y Dermatophagoides siboney. Conclusiones: existe elevada exposición a ácaros del polvo doméstico, particularmente a ácaros de almacén, en las panaderías analizadas, lo que constituye un factor de riesgo de alergia ocupacional a considerar.

  14. [Prevalence of sensitization to inhalant allergens in the immunology department from Hospital de Especialidades Pediátricas María, Honduras, 2016].

    Science.gov (United States)

    Gonzales-González, Victoria Alejandra; Díaz-Flores, Adolfo Martín; Fernández-Zelaya, Karla Zobeyda; Rivera-Reyes, María Félix

    2017-01-01

    Antecedentes: Las enfermedades alérgicas constituyen un problema de salud pública; se estima que entre 30 y 40 % de la población mundial se encuentra afectada por alguna. Conocer la prevalencia de sensibilización a alérgenos permite ofrecer diagnósticos y tratamientos adecuados. En Honduras no se cuenta con estudios en pacientes pediátricos. Objetivos: El objetivo de esta investigación fue identificar el tipo de sensibilización más frecuente en los niños y su correlación con las enfermedades alérgicas más frecuentes en los pacientes en inmunoterapia del Hospital María de Especialidades Pediátricas. Métodos: Estudio retrospectivo transversal descriptivo en el cual se revisaron los expedientes y base de datos de los pacientes en inmunoterapia alérgica del Hospital de Especialidades Pediátricas María entre enero de 2015 y junio de 2016. Resultados: Se evaluaron 215 niños en inmunoterapia, las edades oscilaron entre 3 y 18 años, con una media de 10.8 años. Se identificó que 73.02 % tenía pruebas epicutáneas positivas a aeroalérgenos. Los aeroalérgenos más frecuentes fueron Dermatophagoides farinae y Dermatophagoides psterossynium mix (96.74 %), cucaracha americana (37.21 %) y Aspergillus fumigatus y Homdonderum cladosporioides (16.28 %). Conclusiones: Los ácaros domésticos y la cucaracha americana constituyeron la mayor causa de sensibilización en pacientes en inmunoterapia.

  15. Toxocariasis might be an important cause of atopic myelitis in Korea.

    Science.gov (United States)

    Lee, Jin-Young; Kim, Byoung-Joon; Lee, Sang-Pyo; Jeung, Yun-Jin; Oh, Mi-Jung; Park, Min-Su; Paeng, Jae-Won; Lee, Byung-Jae; Choi, Dong-Chull

    2009-12-01

    Atopic myelitis is defined as myelitis with atopic diasthesis but the cause is still unknown. Toxocariasis is one of the common causes of hyperIgEaemia that may lead to neurologic manifestations. The purpose of this study was to evaluate the sero-prevalence of Toxocara specific IgG Ab among the atopic myelitis patients. We evaluated the medical records of 37 patients with atopic myelitis whose conditions were diagnosed between March 2001 and August 2007. Among them, the 33 sera were analyzed for specific serum IgG Ab to Toxocara excretory-secretory antigens (TES). All of 37 patients had hyperIgEaemia. Specific IgE to D. pteronyssinus and D. farinae was detected in 22 (64.7%) and 34 (100%) patients, respectively, of the 34 patients. Thirty-one of 33 patients (93.9%) were found to be positive by TES IgG enzyme-linked immunosorbent assay (ELISA). Based on the image findings of eosinophilic infiltrations in the lung and liver, 8 patients had positive results. These results inferred that the prevalence of toxocariasis was high in patients with atopic myelitis. Our results suggest that toxocariasis might be an important cause of atopic myelitis and Toxocara ELISA is essential for evaluating the causes of atopic myelitis.

  16. Allergy in the tropics: the impact of cross-reactivity between mites and ascaris.

    Science.gov (United States)

    Caraballo, Luis; Acevedo, Nathalie

    2011-01-01

    Allergic diseases and nematode infections such as ascariasis are important health problems in underdeveloped tropical countries. The co-exposure to Ascaris lumbricoides and the domestic mites Blomia tropicalis and Dermatophagoides pteronyssinus induces a strong Th2 and immunomodulatory responses that can modify the natural history of both diseases. An associate phenomenon of these particular environmental conditions is cross reactivity between mite and Ascaris allergens. We demonstrated a high IgE cross reactivity between the allergenic extracts from both sources and that several already known allergens like tropomyosin and glutathione-s-tranferases are involved. Although this cross reactive antibody response has not been completely analyzed, there are clinical and experimental evidences suggesting that it could be an important component of the complex interactions between ascariasis and mite allergy. For example, it may affect the specificity of serological IgE tests for diagnosing both ascariasis and allergic diseases and, in consequence, the results of epidemiological surveys evaluating the predisposing or protecting role of ascariasis on allergy. In this review we discuss the potential role of cross reactivity on several aspects of allergy in the tropics that have been the matter of a number of investigations, some of them with controversial results.

  17. Surface-modified magnetic colloids for affinity adsorption of immunoglobulins

    Science.gov (United States)

    Martins, Fernanda; Pinho, Samantha C.; Zollner, Terezinha C. A.; Zollner, Ricardo L.; de Cuyper, Marcel; Santana, Maria Helena A.

    This work describes the preparation, characterization and in vitro adsorption tests of surface-modified magnetoliposomes for affinity binding of (i) anticardiolipin (isotype G) antibodies and (ii) specific isotype E antibodies generated by hypersensitivity reactions in humans with respiratory allergy. In the first case, cardiolipin embedded in the bilayer of magnetoliposomes was used as specific ligand. In the second case, antigenic proteins present in an extract of Dermatophagoids pteronyssinus and Blomia tropicalis mites were covalently coupled on the surface of magnetoliposomes via a diglycolic spacer arm, and used as specific ligands for IgE. Antibody adsorption was performed in a high-gradient magnetophoresis system, using either sera of healthy individuals or a pool of sera from autoimmune or allergic patients. The selectivity and capacity of the system were quantified by a frontal analysis in a capillary column, and by constructing breakthrough curves. The results show that the highest yield and selectivity were obtained if the ligand was extended into the aqueous layer surrounding the magnetoliposome surface. A 100% selectivity was obtained for adsorption of specific IgE, and 8% for IgG. These results demonstrate the potentialities of both types of surface-modified magnetic biocolloids in the field of in vitro diagnosis tests for allergic or autoimmune conditions.

  18. The distribution of dust mite allergen in the houses of patients with asthma

    Energy Technology Data Exchange (ETDEWEB)

    Tovey, E.R.; Chapman, M.D.; Wells, C.W.; Platts-Mills, T.A.

    1981-11-01

    Using an inhibition radioimmunoassay for the major allergen from Dermatophagoides pteronyssinus (antigen P1), we studied the distribution of this dust allergen in the houses of patients with asthma. Both bed and floor dust samples contained a wide range of antigen P1, 100 to 100,000 ng/g of fine dust, and this concentration correlated well with the number of mite bodies (r . 0.81, p less than 0.001). We were unable to detect antigen P1 in the air of undisturbed rooms. However, during domestic activity, between 1 and 30 ng were collected on a filter than sampled air for 45 min at 17 L/min. Using a cascade impactor it was shown that greater than 80% of the airborne antigen P1 was associated with particles greater than 10 mu in diameter. Some of the particles containing allergen could be identified because they formed precipitin rings when impacted onto agarose containing rabbit antimite antiserum. These particles had the physical appearance of mite feces, which are the major source of antigen P1 in mite cultures. The results suggested that natural exposure to this dust allergen allows occasional fecal particles to enter the lungs and that these particles contain very concentrated allergen.

  19. Surface-modified magnetic colloids for affinity adsorption of immunoglobulins

    Energy Technology Data Exchange (ETDEWEB)

    Martins, Fernanda [School of Chemical Engineering, State University of Campinas, C.P. 6066, 13083-970 Campinas-SP (Brazil); Pinho, Samantha C. [School of Chemical Engineering, State University of Campinas, C.P. 6066, 13083-970 Campinas-SP (Brazil)], E-mail: samantha@usp.br; Zollner, Terezinha C.A. [School of Chemical Engineering, State University of Campinas, C.P. 6066, 13083-970 Campinas-SP (Brazil); Zollner, Ricardo L. [School of Medical Sciences, State University of Campinas, Campinas-SP (Brazil)], E-mail: zollner@unicamp.br; Cuyper, Marcel de [Interdisciplinary Research Centre, Katholieke Universiteit Leuven-Campus Kortrijk, B-8500 Kortrijk (Belgium)], E-mail: Marcel.DeCuyper@kulak.ac.be; Santana, Maria Helena A. [School of Chemical Engineering, State University of Campinas, C.P. 6066, 13083-970 Campinas-SP (Brazil)], E-mail: lena@feq.unicamp.br

    2008-07-15

    This work describes the preparation, characterization and in vitro adsorption tests of surface-modified magnetoliposomes for affinity binding of (i) anticardiolipin (isotype G) antibodies and (ii) specific isotype E antibodies generated by hypersensitivity reactions in humans with respiratory allergy. In the first case, cardiolipin embedded in the bilayer of magnetoliposomes was used as specific ligand. In the second case, antigenic proteins present in an extract of Dermatophagoids pteronyssinus and Blomia tropicalis mites were covalently coupled on the surface of magnetoliposomes via a diglycolic spacer arm, and used as specific ligands for IgE. Antibody adsorption was performed in a high-gradient magnetophoresis system, using either sera of healthy individuals or a pool of sera from autoimmune or allergic patients. The selectivity and capacity of the system were quantified by a frontal analysis in a capillary column, and by constructing breakthrough curves. The results show that the highest yield and selectivity were obtained if the ligand was extended into the aqueous layer surrounding the magnetoliposome surface. A 100% selectivity was obtained for adsorption of specific IgE, and 8% for IgG. These results demonstrate the potentialities of both types of surface-modified magnetic biocolloids in the field of in vitro diagnosis tests for allergic or autoimmune conditions.

  20. Therapeutic Effects of DNA Vaccine on Allergen-Induced Allergic Airway Inflammation in Mouse Model

    Institute of Scientific and Technical Information of China (English)

    Guoping Li; Zhigang Liu; Nanshan Zhong; Bin Liao1; Ying Xiong

    2006-01-01

    Vaccination with DNA encoding Dermatophagoides pteronyssinus group 2 (Der p 2) allergen previously showed its effects of immunologic protection on Der p 2 allergen-induced allergic airway inflammation in mice. In present study, we investigated whether DNA vaccine encoding Der p 2 could exert therapeutic role on allergen-induced allergic airway inflammation in mouse model and explored the mechanism of DNA vaccination in asthma specific-allergen immunotherapy. After sensitized and challenged by Der p 2, the BALB/c mice were immunized with DNA vaccine. The degrees of cellular infiltration were scored. IgE levels in serum and IL-4/lL-13 levels in BALF were determined by ELISA. The lung tissues were assessed by histological examinations. Expressions of STAT6 and NF-κB in lung were determined by immunohistochemistry staining. Vaccination of mice with DNA vaccine inhibited the development of airway inflammation and the production of mucin induced by allergen, and reduced the level of Der p 2-specific IgE level. Significant reductions of eosinophii infiltration and levels of IL-4and IL-13 in BALF were observed after vaccination. Further more, DNA vaccination inhibited STAT6 and NF-κBexpression in lung tissue in Der p 2-immunized mice. These results indicated that DNA vaccine encoding Der p 2allergen could be used for therapy of allergen-induced allergic airway inflammation in our mouse model.

  1. Comparative analysis of cluster versus conventional immunotherapy in patients with allergic rhinitis.

    Science.gov (United States)

    Fan, Qijun; Liu, Xuejun; Gao, Jinjian; Huang, Saiyu; Ni, Liyan

    2017-02-01

    The present study aimed to compare the efficacy and safety of subcutaneous immunotherapy with Dermatophagoides pteronyssinus standardized extract administered in conventional and cluster immunotherapy regiments for patients with persistent allergic rhinitis. A total of 60 patients with moderate to severe allergic rhinitis caused by dust mites were treated for 1 year with either conventional immunotherapy (n=30) or cluster immunotherapy (n=30). Nasal conjunctival symptoms and signs were assessed to evaluate the clinical efficacy of the two regimens, and the incidence of local and systemic adverse reactions were also evaluated. The findings demonstrated that the cluster regimen reduced the duration between the initial and maintenance dose by >60%, and resulted in a significant improvement, as compared with the conventional regimen, after 6 weeks of observation (Pcluster regimen during the dose accumulation phase and the dose maintenance phase was not significantly different, as compared with the conventional immunotherapy regimen. These results suggest that cluster immunotherapy is efficacious and safe to treat patients who are clinically sensitive to dust mites.

  2. The prevalence, characteristics of and risk factors for eczema in Belgian schoolchildren.

    Science.gov (United States)

    Govaere, Elke; Van Gysel, Dirk; Verhamme, Katia M C; Doli, Erenik; Oranje, Arnold P; De Baets, Frans

    2009-01-01

    Childhood eczema is common in infants, but its nature and extent during later childhood remains unclear. In this cross-sectional study we examined the prevalence and characteristics of eczema in an unbiased community population of 2,021 Belgian schoolchildren, aged 3.4 to 14.8 years with skin prick testing and parental questionnaires. Our study identified an eczema prevalence of 23.3% and a considerable allergic comorbidity, mainly in sensitized children. The reported prevalence of eczema in infancy was 18.5% and for current eczema 11.6%. The overall sensitization rate (33.2%) as well as sensitization rates for the individual allergens were significantly higher in children with "eczema ever." Sensitization to Dermatophagoides pteronyssinus (19.6%), mixed grass pollen (15.1%), and cat (9.1%) were most common. Until the age of 6 years, boys with eczema were significantly more sensitized than girls (p = 0.007). Children with both eczema in infancy and current eczema show a tendency to be more sensitized than children with eczema in infancy only or current eczema only, but significance was only noted for a few individual allergens. Analysis of factors associated with eczema revealed a predominantly atopic profile characterized by family or personal history of allergy. Breastfeeding and environmental factors seemed to assume little relevance except for a protective effect of prematurity and having a dog at birth.

  3. Indoor allergen assessment quantified by a thin-layer electrochemical cell and magnetic beads.

    Science.gov (United States)

    Kurita, Ryoji; Yanagisawa, Hiroyuki; Niwa, Osamu

    2013-10-15

    We report the electrochemical determination of mite allergen in real house dust by using a thin layer electrochemical flow cell and magnetic beads. Der p1, which is an allergen from Dermatophagoides pteronyssinus, was immunochemically sandwiched between two dispersed monoclonal antibodies; one was modified on the surface of magnetic beads and the other was modified with alkaline phosphatase. After washing the beads, a small volume of p-aminophenol phosphate (p-APP) was added to produce p-aminophenol (p-AP). And then the p-AP concentration was measured electrochemically with a homemade electrochemical cell. The Der p1 assay was completed within 30 min and a low detection limit of 0.3 ng/mL was achieved. This is because the diffusion distance of Der p1 and the detection antibody was reduced to 22.3 μm by using dispersed magnetic beads. Only 10 min was required to complete the entire immunoreaction, and 54% of the Der p1 was confirmed to have immunoreacted in only 1 min of mixing. Furthermore, the p-APP volume could be reduced using the thin-layer electrochemical flow cell. This is advantageous in terms of concentrating p-AP, and provides a high signal-to-noise ratio measurement in a short time. We achieved a high correlation (r=0.967, p<0.001) between our assay and a conventional enzyme-linked immunosorbent assay (ELISA) for real house dust measurements.

  4. IMD-4690, a novel specific inhibitor for plasminogen activator inhibitor-1, reduces allergic airway remodeling in a mouse model of chronic asthma via regulating angiogenesis and remodeling-related mediators.

    Directory of Open Access Journals (Sweden)

    Toshifumi Tezuka

    Full Text Available Plasminogen activator inhibitor (PAI-1 is the principal inhibitor of plasminogen activators, and is responsible for the degradation of fibrin and extracellular matrix. IMD-4690 is a newly synthesized inhibitor for PAI-1, whereas the effect on allergic airway inflammation and remodeling is still unclear. We examined the in vivo effects by using a chronic allergen exposure model of bronchial asthma in mice. The model was generated by an immune challenge for 8 weeks with house dust mite antigen, Dermatophagoides pteronyssinus (Dp. IMD-4690 was intraperitoneally administered during the challenge. Lung histopathology, hyperresponsiveness and the concentrations of mediators in lung homogenates were analyzed. The amount of active PAI-1 in the lungs was increased in mice treated with Dp. Administration with IMD-4690 reduced an active/total PAI-1 ratio. IMD-4690 also reduced the number of bronchial eosinophils in accordance with the decreased expressions of Th2 cytokines in the lung homogenates. Airway remodeling was inhibited by reducing subepithelial collagen deposition, smooth muscle hypertrophy, and angiogenesis. The effects of IMD-4690 were partly mediated by the regulation of TGF-β, HGF and matrix metalloproteinase. These results suggest that PAI-1 plays crucial roles in airway inflammation and remodeling, and IMD-4690, a specific PAI-1 inhibitor, may have therapeutic potential for patients with refractory asthma due to airway remodeling.

  5. IMD-4690, a Novel Specific Inhibitor for Plasminogen Activator Inhibitor-1, Reduces Allergic Airway Remodeling in a Mouse Model of Chronic Asthma via Regulating Angiogenesis and Remodeling-Related Mediators

    Science.gov (United States)

    Tezuka, Toshifumi; Ogawa, Hirohisa; Azuma, Masahiko; Goto, Hisatsugu; Uehara, Hisanori; Aono, Yoshinori; Hanibuchi, Masaki; Yamaguchi, Yoichi; Fujikawa, Tomoyuki; Itai, Akiko; Nishioka, Yasuhiko

    2015-01-01

    Plasminogen activator inhibitor (PAI)-1 is the principal inhibitor of plasminogen activators, and is responsible for the degradation of fibrin and extracellular matrix. IMD-4690 is a newly synthesized inhibitor for PAI-1, whereas the effect on allergic airway inflammation and remodeling is still unclear. We examined the in vivo effects by using a chronic allergen exposure model of bronchial asthma in mice. The model was generated by an immune challenge for 8 weeks with house dust mite antigen, Dermatophagoides pteronyssinus (Dp). IMD-4690 was intraperitoneally administered during the challenge. Lung histopathology, hyperresponsiveness and the concentrations of mediators in lung homogenates were analyzed. The amount of active PAI-1 in the lungs was increased in mice treated with Dp. Administration with IMD-4690 reduced an active/total PAI-1 ratio. IMD-4690 also reduced the number of bronchial eosinophils in accordance with the decreased expressions of Th2 cytokines in the lung homogenates. Airway remodeling was inhibited by reducing subepithelial collagen deposition, smooth muscle hypertrophy, and angiogenesis. The effects of IMD-4690 were partly mediated by the regulation of TGF-β, HGF and matrix metalloproteinase. These results suggest that PAI-1 plays crucial roles in airway inflammation and remodeling, and IMD-4690, a specific PAI-1 inhibitor, may have therapeutic potential for patients with refractory asthma due to airway remodeling. PMID:25785861

  6. Allergen-induced migration of human cells in allergic severe combined immunodeficiency mice.

    Science.gov (United States)

    Duez, C; Akoum, H; Marquillies, P; Cesbron, J Y; Tonnel, A B; Pestel, J

    1998-02-01

    Recently, we have shown that severe combined immunodeficiency (SCID) mice, intraperitoneally reconstituted with peripheral blood mononuclear cells (PBMC) from Dermatophagoides pteronyssinus (Dpt)-sensitive patients, produced human IgE and developed a pulmonary inflammatory-type reaction after exposure to allergen aerosol. In order to understand the potential mechanisms involved in the human cell migration in SCID mice, we analysed their phenotypic profile in the lungs, spleen and thymus, 2 months after Dpt inhalation. The human cell recruitment in these organs was found to be allergen-dependent as CD45+ human cells were only detected in hu-SCID mice after Dpt exposure. The composition of the pulmonary human T-cell infiltrate, preferentially memory (CD45RO), activated (human leucocyte antigen (HLA)-DR) and CD4+ cells, was similar to that described in asthmatic patients. However, CD20+ B cells were predominately recruited in the spleen and thymus and may be IgE-producing cells in the spleen. In the lungs, the percentage of human leucocytes expressing the alpha-chain of the lymphocyte function-associated antigen-1 (LFA-1) (CD11a) was higher than those of CD49d+ or CD54+ cells, in contrast to the spleen and thymus, suggesting a potential role of LFA-1 in the human cell migration towards SCID mice lung. In conclusion, this model could be useful in the study of factors implicated in the cellular migration towards the lymphoid organs during an allergic reaction.

  7. Human dendritic cells in the severe combined immunodeficiency mouse model: their potentiating role in the allergic reaction.

    Science.gov (United States)

    Hammad, H; Duez, C; Fahy, O; Tsicopoulos, A; André, C; Wallaert, B; Lebecque, S; Tonnel, A B; Pestel, J

    2000-04-01

    Dendritic cells (DCs) are present in the lungs and airways of healthy and allergic subjects where they are exposed to inhaled antigens. After the uptake of antigens, DCs migrate to lymphoid organs where T cells initiate and control the immune response. The migratory properties of DCs are an essential component of their function but remain unclear in the situation of allergic diseases. To better understand the role of DCs in response to allergens, we first investigated their presence in an original experimental model of allergic asthma: the humanized severe combined immunodeficiency (SCID) mouse reconstituted with peripheral blood mononuclear cells from patients sensitive to Dermatophagoides pteronyssinus (Dpt). Human DCs were detected in lungs of mice developing an inflammatory pulmonary infiltrate and appeared to be mainly located in the alveolar spaces. In a second step, human DCs were generated in vitro from monocytes and injected into naive SCID mice exposed or not exposed to Dpt aerosols. Their migratory behavior was explored, as well as their potential role in modulating the IgE production after exposure to Dpt. After exposure to Dpt, the number of DCs present in airways decreased, while it increased into the spleen and thymus of the mice. The IgE production increased in the presence of DCs as compared with mice not injected with DCs. These results suggest that DCs may play a role in the pulmonary allergic reaction developed in response to Dpt in SCID mice.

  8. Modulation of the allergen-induced human IgE response in Hu-SCID mice: inhibitory effect of human recombinant IFN-gamma and allergen-derived lipopeptide.

    Science.gov (United States)

    Duez, C; Gras-Masse, H; Hammad, H; Akoum, H; Didierlaurent, A; André, C; Tonnel, A B; Pestel, J

    2001-01-01

    We have previously established a model to study the in vivo human IgE response using humanized SCID mice. Allergic SCID mice were obtained following intraperitoneal injection with mononuclear cells from Dermatophagoides pteronyssinus (Dpt)-sensitive patients, and sensitization by Dpt allergen intraperitoneal injection (immunization) or Dpt aerosol (inhalation). Human serum IgE was measured in allergic SCID mice after administration of human recombinant IFN-gamma or the lipopeptide LP 52-71 (derived from peptide p52-71 from Der p 1, Dpt major allergen, coupled to a lipophilic moiety), during the immunization or the inhalation phase. IFN-gamma inhibited human IgE production when given at the time of immunization, but not during inhalation. This effect was long-lasting as Dpt aerosol, given one month after immunization and IFN-gamma administration, failed to increase IgE levels. Unlike Dpt or p52-71, LP 52-71 failed to induce human IgE production at day 14 and 21 after its injection, but did inhibit the development of the IgE response after a secondary Dpt-challenge. Moreover, LP 52-71 administration 14 days after Dpt inhalation decreased IgE levels, in contrast to peptide 52-71, which increased IgE levels. Thus, taken together these results indicate that the development of the human IgE response in allergic SCID mice can be modulated by modified allergen and a Th1 cytokine.

  9. Reduction of the in vivo allergenicity of Der p 2, the major house-dust mite allergen, by genetic engineering.

    Science.gov (United States)

    Chen, Kuan-Wei; Fuchs, Gudrun; Sonneck, Karoline; Gieras, Anna; Swoboda, Ines; Douladiris, Nikolas; Linhart, Birgit; Jankovic, Marija; Pavkov, Tea; Keller, Walter; Papadopoulos, Nikolaos G; Valent, Peter; Valenta, Rudolf; Vrtala, Susanne

    2008-05-01

    The major allergen of the house-dust mite Dermatophagoides pteronyssinus, Der p 2, is recognized by approximately 90% of mite-allergic patients. We have produced two recombinant fragments of Der p 2 comprising aa 1-53 and aa 54-129 and a hybrid molecule (aa 54-129+1-53), combining the two fragments in inverse order, by genetic engineering. The recombinant Der p 2 derivatives were expressed in E. coli and purified to homogeneity. rDer p 2 derivatives (fragments and hybrid) showed a considerably reduced beta sheet structure and IgE reactivity compared to the Der p 2 wild-type allergen. The allergenic activity of the Der p 2 derivatives was reduced more than tenfold as evaluated in vitro in basophil activation assays and in vivo by skin prick testing of mite-allergic patients. Immunization of mice and rabbits with rDer p 2 derivatives induced Der p 2-specific IgG antibodies, which inhibited the binding of allergic patients' IgE to Der p 2. Immunization of mice with rDer p 2 derivatives induced less allergenic IgE responses than immunization with rDer p 2. Thus the rDer p 2 derivatives exhibited less in vivo allergenic activity and allergenicity than the Der p 2 allergen but preserved immunogenicity and may hence represent candidates for specific immunotherapy of house-dust mite allergy.

  10. Papain Degrades Tight Junction Proteins of Human Keratinocytes In Vitro and Sensitizes C57BL/6 Mice via the Skin Independent of its Enzymatic Activity or TLR4 Activation.

    Science.gov (United States)

    Stremnitzer, Caroline; Manzano-Szalai, Krisztina; Willensdorfer, Anna; Starkl, Philipp; Pieper, Mario; König, Peter; Mildner, Michael; Tschachler, Erwin; Reichart, Ursula; Jensen-Jarolim, Erika

    2015-07-01

    Papain is commonly used in food, pharmaceutical, textile, and cosmetic industries and is known to induce occupational allergic asthma. We have previously shown that the papain-like cysteine protease Dermatophagoides pteronyssinus 1 from house dust mite exhibits percutaneous sensitization potential. We aimed here to investigate the potential of papain itself in epicutaneous sensitization. The effects of papain on tight junction (TJ) proteins were tested in vitro in human primary keratinocytes. Using C57BL/6 wild-type and Toll-like receptor 4 (TLR4)-deficient mice, we analyzed the sensitization potential of papain, its effects on the skin barrier, and immune cell recruitment. Our results show that papain affects the skin barrier by increasing transepidermal water loss, degrading TJ proteins and inducing vasodilation. When topically applied, papain exhibited a high epicutaneous inflammatory potential by recruiting neutrophils, mast cells, and CD3-positive cells and by induction of a TH2-biased antibody response. However, its high potency for specific sensitization via the skin was TLR4 independent and, in spite of its capacity to degrade epidermal TJ proteins, does not rely on its enzymatic function. From our data, we conclude that papain has all features to act as a strong allergen via the skin.

  11. IgE cross-reactivity between Ascaris lumbricoides and mite allergens: possible influences on allergic sensitization and asthma.

    Science.gov (United States)

    Acevedo, N; Caraballo, L

    2011-06-01

    Nematode infections such as Ascariasis are important health problems in underdeveloped countries, most of them located in the tropics where environmental conditions also promote the perennial co-exposure to high concentrations of domestic mite allergens. Allergic diseases are common, and most of patients with asthma exhibit a predominant and strong IgE sensitization to mites. It is unknown whether co-exposure to Ascaris lumbricoides and the domestic mites Blomia tropicalis and Dermatophagoides pteronyssinus potentiates Th2 responses and IgE sensitization, thereby modifying the natural history of allergy. Recently, we obtained experimental evidence of a high cross-reactivity between the allergenic extracts of these invertebrates, involving well-known allergens such as tropomyosin and glutathione transferases. There is indirect evidence suggesting that the clinical impact of these findings may be important. In this review, we discuss the potential role of this cross-reactivity on several aspects of allergy in the tropics that have been a focus of a number of investigations, some of them with controversial results.

  12. Ingestion of milk containing the Dp2 peptide, a dust mite allergen, protects mice from allergic airway inflammation and hyper-responsiveness

    Science.gov (United States)

    2013-01-01

    Background Allergen-specific immunotherapy has been demonstrated to have potential for the treatment of allergic diseases. Transgenic animals are currently the best available bioreactors to produce recombinant proteins, which can be secreted in milk. It has not been clearly demonstrated whether milk from transgenic animals expressing recombinant allergens has immunomodulatory effects on allergic asthma. Methods We aimed to determine whether the oral administration of milk containing a mite allergen can down-regulate allergen-specific airway inflammation. Transgenic CD-1 mice that express a recombinant group 2 allergen from Dermatophagoides pteronyssinus (Dp2) in their milk were generated using an embryonic gene-microinjection technique. Mouse pups were fed transgenic Dp2-containing milk or wild-type milk. Subsequently, these mice were sensitized and challenged with Dp2 to induce allergic airway inflammation. Results Upon sensitization and challenge, mice fed transgenic Dp2 milk had decreased T-helper 2 (Th2) and increased T-helper 1 (Th1) responses in the airway compared with mice fed wild-type milk. Moreover, pre-treatment with transgenic Dp2 milk attenuated airway inflammation and decreased airway hyper-responsiveness. Conclusions This study provides new evidence that oral administration of transgenic milk containing the Dp2 allergen down-regulated and moderately protected against allergic airway inflammation. Milk from transgenic animals expressing allergens may have potential use in the prevention of allergic asthma. PMID:23763898

  13. Stability evaluation of house dust mite vaccines for sublingual immunotherapy

    Directory of Open Access Journals (Sweden)

    MARIJA GAVROVIĆ-JANKULOVIĆ

    2010-01-01

    Full Text Available Allergen-specific immunotherapy with house dust mite (HDM allergen extracts can effectively alleviate the symptoms of allergic rhinitis and asthma. The efficacy of the immunotherapeutic treatment is highly dependent on the quality of house dust mite vaccines. This study was performed to assess the stability of house dust mite allergen vaccines prepared for sublingual immunotherapy. Lyophilized Dermatophagoides pteronyssinus (Dpt mite bodies were the starting material for the production of sublingual vaccines in four therapeutic concentrations. The stability of the extract for vaccine production, which was stored below 4 °C for one month, showed consistence in the protein profile in SDS PAGE. ELISA-inhibition showed that the potencies of Dpt vaccines during a 12 month period were to 65–80 % preserved at all analyzed therapeutic concentrations. This study showed that glycerinated Dpt vaccines stored at 4 °C preserved their IgE-binding potential during a 12 month period, implying their suitability for sublingual immunotherapeutic treatment of HDM allergy.

  14. High-level expression and purification of the major house dust mite allergen Der p 2 in Escherichia coli.

    Science.gov (United States)

    Cao, Tuo; Zhang, Zhang; Liu, Zhi-Gao; Dou, Xia; Zhang, Jie; Zhang, Wei; Wu, Bo; Yu, Zhen-Dong; Wei, Zhun; Yu, Bo

    2016-05-01

    Der p 2, a major allergen derived from the house dust mite Dermatophagoides pteronyssinus, is one of the most clinically relevant allergens worldwide. Recombinant Der p 2 (rDer p 2) is useful in clinical diagnosis and disease-specific immunotherapy. However, previous studies showed that Der p 2 can only be expressed in Escherichia coli (E. coli) cells as inclusion bodies, thus protein refolding is required to obtain functional products. Here we report a new method to produce biologically active Der p 2 protein in E. coli. N-terminal hexahistidine- and trigger factor (TF)-tagged Der p 2 was expressed in soluble form in E. coli and purified using a combination of chromatography processes. This procedure produced milligram-level high purity Der p 2 per liter of bacterial culture. Moreover, far-UV region circular dichroism (CD) analysis and serum specific IgE reactivity test demonstrated that the secondary structure and IgE reactivity properties of rDer p 2 produced in our study were almost identical to those of natural Der p 2 (nDer p 2). In conclusion, the method developed in this work provides a useful tool for the production of immunologically active recombinant Der p 2 for clinical applications.

  15. Association Between Aeroallergens and Allergic Diseases Based on Skin Prick Test in Bushehr Province

    Directory of Open Access Journals (Sweden)

    Ali Moradi

    2017-01-01

    Full Text Available Background: The prevalence of allergic diseases is growing in the world and Iran. Allergens play an important role in creating these diseases. Since inhalation allergens are an important part of these allergens the aim of this study was to assess the prevalence of aeroallergens and their association with allergic diseases based on skin prick test (SPT. Material and Methods: In this study, the frequency of these allergens were examined by using 22 aeroallergens and based on Skin Prick Test in 1100 patients with allergic diseases who referred to Shohadaye- Khalije- Fars university hospital. Results: The reaction severity of SPT and the severity of disease associated with inhaled allergens with Russian thistle (21.1%, P꞊0.001, Chenopodium album pollen (14%, P꞊0.001 and Dermatophagoid Pteronyssinus (9%, P꞊0.001 respectively. Also, in these patients, the frequency of inhalation allergens were House Dust Mite (HDM (69%, feather (60.8%, Russian this tle (59.9% pollen and Alternaria mold (59.6% respectively. Conclusion: This study indicated that outdoors inhaled allergens  such as Russian thistle and Chenopodium album pollen have the most allergenicity in patients with allergic and asthma in Bushehr province, Meanwhile, the prevalence of indoor aeroallergens such as HDM, feather and mold were high in these patients.

  16. Zinc sulfate inhibited inflammation of Der p2-induced airway smooth muscle cells by suppressing ERK1/2 and NF-κB phosphorylation.

    Science.gov (United States)

    Shih, Chia-Ju; Chiou, Ya-Ling

    2013-06-01

    Inflammation of airway smooth muscle cells (ASMCs) is believed to be important in causing airway hyperresponsiveness. However, zinc has been reported to be implicated in many kinds of cell inflammation. Little is known about the effect of zinc treatment on Der p2 (group II Dermatophagoides pteronyssinus)-induced inflammation from ASMCs. This study investigated effects and mechanisms of zinc in Der p2-treated ASMCs. Der p2-treated primary ASMCs were cultured with various concentrations of zinc sulfate (ZnSO₄) 6 μM, 12 μM, 24 μM, and 96 μM. The proteins and mRNAs of cytokines in ASMCs were examined by ELISA and real-time PCR. Intracellular zinc was stained with Zinquin fluorescence. The cell signaling protein expression was detected by Western blot. Der p2 was used to induce interleukin (IL)-6, IL-8, IL-1, and monocyte chemotactic protein-1 production of ASMCs. However, we found that 24 μM ZnSO₄ reduced these inflammatory mediators production of Der p2-treated primary ASMCs. Der p2-induced extracellular signal-regulated kinases (ERK) and nuclear factor-kappa B (NF-κB) phosphorylation were suppressed by supplementation of 24 μM ZnSO₄. Zinc is an anti-inflammatory agent that reduces inflammation of Der p2-treated ASMCs through the suppression of the ERK and NF-κB pathway. The results may be helpful for the development of effective treatments.

  17. A CCL24-dependent pathway augments eosinophilic airway inflammation in house dust mite-challenged Cd163(-/-) mice.

    Science.gov (United States)

    Dai, C; Yao, X; Gordon, E M; Barochia, A; Cuento, R A; Kaler, M; Meyer, K S; Keeran, K J; Nugent, G Z; Jeffries, K R; Qu, X; Yu, Z-X; Aponte, A; Gucek, M; Dagur, P K; McCoy, J P; Levine, S J

    2016-05-01

    CD163 is a macrophage scavenger receptor with anti-inflammatory and pro-inflammatory functions. Here, we report that alveolar macrophages (AMΦs) from asthmatic subjects had reduced cell-surface expression of CD163, which suggested that CD163 might modulate the pathogenesis of asthma. Consistent with this, house dust mite (HDM)-challenged Cd163(-/-) mice displayed increases in airway eosinophils and mucous cell metaplasia (MCM). The increased airway eosinophils and MCM in HDM-challenged Cd163(-/-) mice were mediated by augmented CCL24 production and could be reversed by administration of a neutralizing anti-CCL24 antibody. A proteomic analysis identified the calcium-dependent binding of CD163 to Dermatophagoides pteronyssinus peptidase 1 (Der p1). Der p1-challenged Cd163(-/-) mice had the same phenotype as HDM-challenged Cd163(-/-) mice with increases in airway eosinophils, MCM and CCL24 production, while Der p1 induced CCL24 secretion by bone marrow-derived macrophages (BMMΦs) from Cd163(-/-) mice, but not BMMΦs from wild-type (WT) mice. Finally, airway eosinophils and bronchoalveolar lavage fluid CCL24 levels were increased in Der p1-challenged WT mice that received adoptively transferred AMΦ's from Cd163(-/-) mice. Thus, we have identified CD163 as a macrophage receptor that binds Der p1. Furthermore, we have shown that HDM-challenged Cd163(-/-) mice have increased eosinophilic airway inflammation and MCM that are mediated by a CCL24-dependent mechanism.

  18. Orchestration of an uncommon maturation cascade of the house dust mite protease allergen quartet

    Directory of Open Access Journals (Sweden)

    Marie-Eve eDumez

    2014-03-01

    Full Text Available In more than 20% of the world population, sensitization to house dust mite (HDM allergens triggers typical allergic diseases such as allergic rhinitis and asthma. Amongst the 23 mite allergen groups hitherto identified, groups 1 are cysteine proteases belonging to the papain-like family whereas groups 3, 6 and 9 are serine proteases displaying trypsin, chymotrypsin and collagenolytic activities, respectively. While these proteases are more likely to be involved in the mite digestive system, they also play critical roles in the initiation and in the chronicity of the allergic response notably through the activation of innate immune pathways. All these allergenic proteases are expressed in mite as inactive precursor form. Until recently, the exact mechanisms of their maturation into active proteases remained to be fully elucidated. Recent breakthroughs in the understanding of the activation mechanisms of mite allergenic protease precursors have highlighted an uncommon and unique maturation pathway orchestrated by group 1 proteases that tightly regulates the proteolytic activities of groups 1, 3, 6 and 9 through complex intra- or intermolecular mechanisms. This review presents and discusses the currently available knowledge of the activation mechanisms of group 1, 3, 6 and 9 allergens of Dermatophagoides pteronyssinus laying special emphasis on their localization, regulation and interconnection.

  19. Studies on bronchial hyperreactivity, allergic responsiveness, and asthma in rural and urban children of the highlands of Papua New Guinea.

    Science.gov (United States)

    Turner, K J; Dowse, G K; Stewart, G A; Alpers, M P

    1986-04-01

    The prevalence of asthma and allergic responsiveness in rural and urban children of the highlands of Papua New Guinea was studied. Bronchial provocation studies with histamine demonstrated significant bronchial hyperreactivity in 0.5% (1 in 195) rural and 1.7% (1 in 59) urban children, rates which were significantly lower than those observed in corresponding adult populations (7%). Urban children demonstrated a higher incidence of skin test reactivity toward Dermatophagoides pteronyssinus, Aspergillus fumigatus, and dog dander than did the rural children. However, there were no significant differences between these populations with regard to total serum IgE levels, the degree of parasitism as judged by stool examination, or allergic responses to Ascaris suum, plantain, and coffee bean husk. A more detailed study demonstrated age- and sex-related differences in total IgE and mite-specific RAST scores in the rural but not the urban population. These data suggest an active suppression of the capacity of children to mount an IgE response to environmental allergens such as the mite manifesting itself as low asthma prevalence. The data also indicate that, although the underlying defect of bronchial hyperreactivity in asthma may be genetically inherited, it is not revealed until the lung has received an allergen-induced inflammatory insult.

  20. Dust mite allergen, glutathione S-transferase, induces T cell immunoglobulin mucin domain-4 in dendritic cells to facilitate initiation of airway allergy.

    Science.gov (United States)

    Mo, L-H; Yang, L-T; Zeng, L; Xu, L-Z; Zhang, H-P; Li, L-J; Liu, J-Q; Xiao, X-J; Zheng, P-Y; Liu, Z-G; Yang, P-C

    2017-02-01

    Allergens from dust mites play a critical role in the pathogenesis of airway allergy. The mechanism by which dust mite allergens induce allergic diseases is not fully understood yet. This study tests a hypothesis that the eighth subtypes of Dermatophagoides farina allergen (Derf8) play an important role in the induction of airway allergy. The protein of Derf8 was synthesized via molecular cloning approach. Dendritic cells (DC) were stimulated with Derf8 in the culture, and then, the expression of T cell immunoglobulin mucin domain 4 (TIM4) in dendritic cells (DC) was analysed. The role of Derf8 in the induction of airway allergy was evaluated with a mouse model. Exposure to Derf8 markedly induced the TIM4 expression in DCs by modulating the chromatin at the TIM4 promoter locus. Derf8 played a critical role in the expansion of the T helper 2 response in the mouse airway via inducing DCs to produce TIM4. Administration with Derf8-depleted dust mite extracts (DME) inhibited the allergic inflammation and induced regulatory T cells in mice with airway allergy. Derf8 plays an important role in the initiation of dust mite allergy. Vaccination with Derf8-deficient DME is more efficient to inhibit the dust mite allergic inflammation than using wild DME. © 2016 John Wiley & Sons Ltd.

  1. Science Letters: A synthetic Toll-like receptor 2 ligand decreases allergic immune responses in a mouse rhinitis model sensitized to mite allergen

    Institute of Scientific and Technical Information of China (English)

    Cheng ZHOU; Xiao-dong KANG; Zhi CHEN

    2008-01-01

    It has been proposed that activation of Toll-like receptors (TLRs) plays crucial roles in the polarization of adaptive immune responses. A synthetic Toll-like receptor 2 (TLR2) ligand, Pam3CSK4, has been reported to modulate the balance of Thl/Tn2 responses. We evaluated the modulation effect of Pam3CSK4 on allergic immune response in a mouse rhinitis model sensitized to house dust mite allergen (HDM). Mice were sensitized and challenged with Dermatophagoides farinae allergen (Der f), and then the allergic mice were treated by Pam3CSK4. Nasal allergic symptoms and eosinophils were scored. Der f-specific cytokine responses were examined in the splenocytes and bronchoalveolar lavage fluid (BALF). Serum level of total IgE was also detected. After establishing a mouse allergic rhinitis model with HDM, we have showed that Pam3CSK4 treatment not only ameliorated the nasal allergic symptoms remarkably but also decreased the eosinophils and total inflammation cells in BALF significantly. Analysis of cytokine profile found that' IFN-γ released from either BALF or stimulated splenocytes increased markedly in Pam3CSK4-treated mice, while IL-13 decreased significantly. Moreover, serum level of total IgE was significantly lower in Pam3CSK4-treated mice than in the untreated. Thus, in an allergic rhinitis mouse model developed with HDM, Pam3CSK4 was shown to exhibit an antiallergic effect, indicating its potential application in allergic diseases.

  2. Interleukin-10 inhibits the production of inflammatory cytokines by antigen-stimulated mononuclear cells from asthmatic patients

    Directory of Open Access Journals (Sweden)

    Toshiya Takahashi

    2000-01-01

    Full Text Available Bronchial asthma, characterized by chronic airway inflammation, involves many inflammatory cytokines. Interleukin (IL-10 is a potent inhibitor of cytokine synthesis. Thus, the effects of IL-10 were examined on the production of granulocyte-macrophage colony stimulating factor (GM-CSF, IL-5, IL-1 β, IL-2 and interferon (IFN-γ by antigen (Dermatophagoides farinae, Df- stimulated mononuclear cells obtained from asthmatic patients who were sensitized with the antigen and from healthy subjects in vitro. Production of IL-5 and IL-2 was enhanced by Df antigen in the asthmatic subjects, but not in the healthy controls. In contrast, levels of GM-CSF, IFN-γ and IL-1 β production were enhanced by the antigen in both groups. Exogenous IL-10 (10 ng/mL inhibited the production of GM-CSF, IFN-γ and IL-iβ induced by Df antigen in both groups and also inhibited the production of IL-5 and IL-2 induced by the antigen in the asthmatics subjects. The inhibition of GM-CSF production by IL-10 was stronger than that by IL-4. These results indicated that the responsiveness to the inhibitory effect of IL-10 on the production of inflammatory cytokines is not abrogated in asthmatic patients and that IL-10 may be useful in the treatment of bronchial asthma.

  3. Heat-Killed Enterococcus faecalis EF-2001 Ameliorates Atopic Dermatitis in a Murine Model

    Directory of Open Access Journals (Sweden)

    Eun-Ju Choi

    2016-03-01

    Full Text Available Recent reports have shown the immunomodulatory effect of heat-killed lactic acid bacteria. Atopic dermatitis (AD is an allergic skin disease, caused by immune dysregulation among other factors. The aim of this study was to assess the effect of heat-killed Enterococcus faecalis EF-2001 (EF-2001 on AD. We established an in vivo AD model by repeated local exposure of Dermatophagoides farinae extract (DFE; house dust mite extract and 2,4-dinitrochlorobenzene (DNCB to the ears of mice. After oral administration of EF-2001 for four weeks, the epidermal and dermal ear thickness, mast cell infiltration, and serum immunoglobulin levels were measured. In addition, the gene expression levels of pathogenic cytokines in the ears, lymph nodes, and splenocytes were assayed. EF-2001 attenuated AD symptoms based on the ear thickness, histopathological analysis, and serum immunoglobulin levels. Moreover, EF-2001 decreased the DFE/DNCB-induced expression of various pathogenic cytokines in the ears, lymph nodes, and splenocytes. These results suggest that EF-2001 has therapeutic potential in the treatment of AD owing to its immunomodulatory effects.

  4. The Hot-Water Extract of Smilacis Chinae Rhizome Suppresses 2,4-Dinitrochlorobenzene and House Dust Mite-Induced Atopic Dermatitis-Like Skin Lesions in Mice.

    Science.gov (United States)

    Ki, Nam Yong; Park, Eun-Ji; Sung, In sung; Ju, Seul A; Kim, Kyoung Un; Kim, Mi Rae; Song, Do Yeon; Lee, Min-Ju; Kim, Hak-Soo; Kang, Boo-Hyon; Chung, Hun-Jong; Choi, Eun-Ju; Yoon, Ki-Hun; Lee, Min Won; Yun, Seongho; Min, Bokkee; Kwon, Suk Hyung; Shin, Hwa-Sup

    2016-04-01

    Smilacis Chinae Rhizome (SCR) has been used as an oriental folk medicine for various biological activities. However, its effect on atopic dermatitis (AD) remains undetermined to date. We assessed the effect of orally administered hot-water extract of SCR on AD-like skin lesions in mice and its underlying mechanisms. AD-like murine model was prepared by repeated alternate application of house dust mite (Dermatophagoides farinae) extract (DFE) and 2,4-dinitrochlorobenzene (DNCB) for 4 weeks, topically to the ears. Daily oral administration of SCR for 3 and 4 weeks significantly reduced inflammatory ear thickening, with the effect being enhanced at the earlier start and longer period of administration. This effect was accompanied by a significant decrease in both Th2 and Th1 serum antibodies (total IgE, DFE-specific IgE, and IgG2a). Histological analysis showed that SCR markedly decreased the epidermal/dermal ear thickening and the dermal infiltration of inflammatory cells. Furthermore, SCR suppressed DFE/DNCB-induced expression of IL-4, IL-13, IL-17, IL-18, TSLP, and IFN-γ genes in the ear tissue. Taken together, our observations demonstrate that chronic oral administration of SCR exerts beneficial effect in mouse AD model, suggesting that SCR has the therapeutic potential as an orally active treatment of AD by modulating both Th1 and Th2 responses.

  5. Allergic rhinitis and co-morbid asthma: perspective from India -- ARIA Asia-Pacific Workshop report.

    Science.gov (United States)

    Shah, Ashok; Pawankar, Ruby

    2009-03-01

    In India, allergic rhinitis (AR) is considered to be a trivial disease, despite the fact that symptoms of rhinitis were present in 75% of children and 80% of asthmatic adults. Traditionally, AR was also divided into seasonal or perennial, based on the time of occurrence of symptoms during the year. The ARIA workshop report proposed that patients be categorized as "intermittent" and "persistent" while severity was classified as "mild" and "moderate-severe". Patients with AR, depending on their predominant symptom, can also be categorized as "sneezers-runners" and "blockers". On sketching their clinical profile, it was observed that "blockers" had significantly higher sinusitis and had higher sensitization to fungi. Skin allergy testing in Indian adults showed that in patients with AR house dust mite (Dermatophagoides farinae) was the most common allergen. Studies conducted in India have shown that AR often restricts the patient's quality of life (QOL). It can affect the physical, psychological and social aspects of the patients' life and can also impact their functions at work. Furthermore, AR adversely affects sleep related QOL. Topical corticosteroids are now considered as the cornerstone of the treatment for AR. In spite of causing a major impact on the QOL in Indian patients, AR is rarely given the importance it deserves.

  6. Salvia plebeia suppresses atopic dermatitis-like skin lesions.

    Science.gov (United States)

    Choi, Jin Kyeong; Oh, Hyun-Mee; Lee, Soyoung; Kwon, Taeg Kyu; Shin, Tae-Yong; Rho, Mun-Chual; Kim, Sang-Hyun

    2014-01-01

    Salvia plebeia R. Br. (Lamiaceae) has been used for folk medicines in Asian countries, including Korea and China, to treat skin inflammatory diseases and asthma. In this study, we investigated the effects of S. plebeia extract (SPE) on atopic dermatitis (AD)-like skin lesions and defined underlying mechanisms of action. We established an AD model in BALB/c mice by repeated local exposure of house dust mite extract (Dermatophagoides farinae extract, DFE) and 2,4-dinitrochlorobenzene (DNCB) to the ears. Repeated alternative treatment of DFE/DNCB caused AD-like skin lesions. The oral administration of SPE decreased AD symptoms based on ear thickness and histopathological analysis, in addition to serum IgE and IgG2a levels. SPE suppressed mast cell infiltration into the ear and serum histamine level. SPE inhibited Th1/Th2/Th17 phenotype CD4(+) T lymphocytes expansion in the lymph node and the expression of Th1/Th2/Th17 cytokines in the ear tissue. To define the underlying mechanisms of action, the tumor necrosis factor (TNF)-α and interferon (IFN)-γ activated human keratinocytes (HaCaT) model was used. SPE significantly suppressed the expression of cytokines and chemokines through the down-regulation of mitogen-activated protein kinases, nuclear factor-κB, and STAT1 in HaCaT cells. Taken together, our results suggest that SPE might be a candidate for the treatment of AD.

  7. A chestnut seed cystatin differentially effective against cysteine proteinases from closely related pests.

    Science.gov (United States)

    Pernas, M; Sánchez-Monge, R; Gómez, L; Salcedo, G

    1998-12-01

    Cystatin CsC, a cysteine proteinase inhibitor from chestnut (Castanea sativa) seeds, has been purified and characterized. Its full-length cDNA clone was isolated from an immature chestnut cotyledon library. The inhibitor was expressed in Escherichia coli and purified from bacterial extracts. Identity of both seed and recombinant cystatin was confirmed by matrix-assisted laser desorption/ionization mass spectrometry analysis, two-dimensional electrophoresis and N-terminal sequencing. CsC has a molecular mass of 11,275 Da and pI of 6.9. Its amino acid sequence includes all three motifs that are thought to be essential for inhibitory activity, and shows significant identity to other phytocystatins, especially that of cowpea (70%). Recombinant CsC inhibited papain (Ki 29 nM), ficin (Ki 65 nM), chymopapain (Ki 366 nM), and cathepsin B (Ki 473 nM). By contrast with most cystatins, it was also effective towards trypsin (Ki 3489 nM). CsC is active against digestive proteinases from the insect Tribolium castaneum and the mite Dermatophagoides farinae, two important agricultural pests. Its effects on the cysteine proteinase activity of two closely related mite species revealed the high specificity of the chestnut cystatin.

  8. Repetitive Immunoassay with a Surface Acoustic Wave Device and a Highly Stable Protein Monolayer for On-Site Monitoring of Airborne Dust Mite Allergens.

    Science.gov (United States)

    Toma, Koji; Miki, Daisuke; Kishikawa, Chisato; Yoshimura, Naoyuki; Miyajima, Kumiko; Arakawa, Takahiro; Yatsuda, Hiromi; Mitsubayashi, Kohji

    2015-10-20

    This work describes a sensor to be incorporated into the on-site monitoring system of airborne house dust mite (HDM) allergens. A surface acoustic wave (SAW) device was combined with self-assembled monolayers of a highly stable antibody capture protein on the SAW surface that have high resistance to pH change. A sandwich assay was used to measure a HDM allergen, Der f 1 derived from Dermatophagoides farinae. Capture antibodies were cross-linked to a protein G based capture layer (ORLA85) on the sensor surface, thereby only Der f 1 and detection antibodies were regenerated by changing pH, resulting in fast repetition of the measurement. The sensor was characterized through 10 repetitive measurements of Der f 1, which demonstrated high reproducibility of the sensor with the coefficient of variation of 5.6%. The limit of detection (LOD) of the sensor was 6.1 ng·mL(-1), encompassing the standard (20 ng·mL(-1)) set by the World Health Organization. Negligible sensor outputs were observed for five different major allergens including other HDM allergens which tend to have cross-reactivity to Der f 1 and their mixtures with Der f 1. Finally, the sensor lifetime was evaluated by conducting three measurements per day, and the sensor output did not substantially change for 4 days. These characteristics make the SAW immunosensor a promising candidate for incorporation into on-site allergen monitoring systems.

  9. Influence of Asian dust particles on immune adjuvant effects and airway inflammation in asthma model mice.

    Directory of Open Access Journals (Sweden)

    Jun Kurai

    Full Text Available An Asian dust storm (ADS contains airborne particles that affect conditions such as asthma, but the mechanism of exacerbation is unclear. The objective of this study was to compare immune adjuvant effects and airway inflammation induced by airborne particles collected on ADS days and the original ADS soil (CJ-1 soil in asthma model mice.Airborne particles were collected on ADS days in western Japan. NC/Nga mice were co-sensitized by intranasal instillation with ADS airborne particles and/or Dermatophagoides farinae (Df, and with CJ-1 soil and/or Df for 5 consecutive days. Df-sensitized mice were stimulated with Df challenge intranasally at 7 days after the last Df sensitization. At 24 hours after challenge, serum allergen specific antibody, differential leukocyte count and inflammatory cytokines in bronchoalveolar lavage fluid (BALF were measured, and airway inflammation was examined histopathologically.Co-sensitization with ADS airborne particles and Df increased the neutrophil and eosinophil counts in BALF. Augmentation of airway inflammation was also observed in peribronchiolar and perivascular lung areas. Df-specific serum IgE was significantly elevated by ADS airborne particles, but not by CJ-1 soil. Levels of interleukin (IL-5, IL-13, IL-6, and macrophage inflammatory protein-2 were higher in BALF in mice treated with ADS airborne particles.These results suggest that substances attached to ADS airborne particles that are not in the original ADS soil may play important roles in immune adjuvant effects and airway inflammation.

  10. Topical application of Moringa oleifera leaf extract ameliorates experimentally induced atopic dermatitis by the regulation of Th1/Th2/Th17 balance.

    Science.gov (United States)

    Choi, Eun-Ju; Debnath, Trishna; Tang, Yujiao; Ryu, Young-Bae; Moon, Sang-Ho; Kim, Eun-Kyung

    2016-12-01

    Moringa oleifera (M. oleifera) is widely cultivated in tropical and subtropical regions and has been used as a vegetable and in traditional medicine. In this study, the anti-atopic dermatitis activity of the ethanol extract of M. oleifera leaf was investigated in vitro and in vivo. For the in vitro study, HaCaT human keratinocytes were used for cytokines and MAPKinase assay. In the in vivo study, M. oleifera leaf ethanolic extract (MO) was topically applied to BALB/c mice with Dermatophagoides farinae extract (DFE; house dust mite extract)- and 2,4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis (AD). The expression of TNF-α, CCL17, IL-1β, IL-6 pro-inflammatory cytokine-related mRNA, and mitogen-activated protein kinases (MAPKs) in TNF-α/IFN-γ-induced HaCaT keratinocytes were reduced by MO. Epidermal and dermal ear thickness, mast cell infiltration, serum immunoglobulin levels, as well as gene expression of various cytokines in the ear tissue, lymph nodes, and splenocytes were improved by treatment with MO. In addition, MO reduced the expression of retinoic acid-related orphan receptor γT (RORγT), thymic stromal lymphopoietin (TSLP), and mannose receptor (CD206) mRNA in the ear tissue and improved cervical lymph node size. The results of this study strongly suggest the beneficial effects of MO on AD via the regulation of inflammatory responses. Copyright © 2016. Published by Elsevier Masson SAS.

  11. Prominent IgE-binding and cytokine-inducing capacities of a newly cloned N-terminal region of Der f 14, an apolipophorin-like house dust mite allergen.

    Science.gov (United States)

    ElRamlawy, Kareem Gamal; Fujimura, Takashi; Aki, Tsunehiro; Okada, Akiyasu; Suzuki, Takayuki; Abe, Takuya; Hayashi, Takaharu; Epton, Michael J; Thomas, Wayne R; Rafeet, Inas Hussein; Al-Azhary, Diaa Beshr; Ono, Kazuhisa; Kawamoto, Seiji

    2017-09-11

    We previously characterized a 177 kDa allergen, M-177, from Dermatophagoides farinae. Thereafter, a counterpart to M-177 for Euroglyphus maynei was cloned as Eur m 14, and its sequence revealed that two environmental allergens, Mag 1 and Mag 3, are digested fragments of M-177. The aims of this study were to clone the cDNA of Der f 14 corresponding to M-177 and to elucidate the allergenic capacities of the N-terminal fragment of Der f 14 (Der f 14-N). Recombinant allergens were produced as trigger-factor-fused proteins in Escherichia coli. Der f 14-N showed the highest IgE-binding frequency among Der f 14-derived fragments in patients allergic to house dust mite by enzyme-linked immunosorbent assay. Der f 14-N showed the highest capacity to induce cell proliferation in murine lymphocyte and human peripheral mononuclear cells among Der f 14-derived fragments. Der f 14-N induced IL-13, IFN-γ, and IL-17 production more than Der f 1 and Der f 2 in mouse, and induced IL-5 and IFN-γ production at levels comparable to those of Der f 1 and Der f 2 in some patients. The high prevalence of IgE binding to the Der f 14-N indicates that it could be an important mite allergen. © The Authors 2017. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved.

  12. Stratum corneum removal facilitates experimental sensitization to mite allergens in atopic dogs.

    Science.gov (United States)

    Olivry, Thierry; Wofford, Jessica; Paps, Judy S; Dunston, Stanley M

    2011-04-01

    In humans with atopic dermatitis and in mouse models of IgE-mediated allergic diseases, evidence is mounting that the stratum corneum (SC) provides an important barrier against environmental allergens. At this time, it is not known whether the SC has a similar role in dogs, especially in those with atopic dermatitis. The objectives of this pilot study were to determine whether SC removal led to earlier and stronger sensitization of atopic dogs to Dermatophagoides farinae (Df) house dust mites. Five Maltese-beagle atopic (MBA) dogs were sensitized epicutaneously after the SC was removed with ten tape strips (TS group), while sensitization was done without tape strips in five other MBA dogs (nontape stripping; NTS group). During this 16 week study, sensitization was assessed with allergen-specific IgE serology, intradermal testing with Df allergens and determination of stimulation indices of blood mononuclear cells cultured with Df and stained for CD4 and the activation markers CD25 or CD30. Compared with dogs from the NTS group, those of the TS group exhibited earlier rises in Df-specific IgE serum levels, usually had higher allergen-specific IgE titres, showed higher intradermal test reactivity and had earlier increases and higher percentages of CD25- or CD30-positive activated allergen-specific peripheral CD4-positive T lymphocytes. These observations implicate a role of the SC as a barrier limiting sensitization to exogenous allergens in this experimental atopic dog model.

  13. Influence of Epinastine Hydrochloride, an H1-Receptor Antagonist, on the Function of Mite Allergen-Pulsed Murine Bone Marrow-Derived Dendritic Cells In Vitro and In Vivo

    Directory of Open Access Journals (Sweden)

    Ken-Zaburo Oshima

    2009-01-01

    Full Text Available There is established concept that dendritic cells (DCs play essential roles in the development of allergic immune responses. However, the influence of H1 receptor antagonists on DC functions is not well defined. The aim of the present study was to examine the effect of epinastine hydrochloride (EP, the most notable histamine H1 receptor antagonists in Japan, on Dermatophagoides farinae (Der f-pulsed mouse bone marrow-derived DCs in vitro and in vivo. EP at more than 25 ng/mL could significantly inhibit the production of IL-6, TNF- and IL-10 from Der f-pulsed DCs, which was increased by Der f challenge in vitro. On the other hand, EP increased the ability of Der f-pulsed DCs to produce IL-12. Intranasal instillation of Der f-pulsed DCs resulted in nasal eosinophilia associated with a significant increase in IL-5 levels in nasal lavage fluids. Der f-pulsed and EP-treated DCs significantly inhibited nasal eosinophila and reduced IL-5. These results indicate that EP inhibits the development of Th2 immune responses through the modulation of DC functions and results in favorable modification of clinical status of allergic diseases.

  14. 居室尘埃内毒素水平对广东城市和农村地区青少年气道反应性及特应性的影响%The influence of house dust endotoxin on airway responsiveness and allergen sensitization in adolescence in urban and rural areas of Guangdong province

    Institute of Scientific and Technical Information of China (English)

    潘利英; 冯木林; 赖旭新; 鲜墨; 黄夏飞; 陈燕; 李靖

    2012-01-01

    Objective To study the association between house dust endotoxin levels and the airway responsiveness as well as allergen sensitization in adolescence in the urban and rural areas of Guangdong province.Methods Middle school students,aged 13-14,188 (male 109,female 79) from Guangzhou,129(male 70,female 59) from Conghua with positive responses,and 231 (male 125,female 106) and 307(male 145,female 162) of their respective reference students are selected from questionnaire prescreening.A detailed questionnaire,the lung function test,histamine bronchial provocation test,skin prick test and peripheral blood eosinophil count are undertaken.Seventy-six students from Guangzhou and 80 from Conghua were selected randomly for mattress dust sample collection to detect the levels of endotoxin.Results The prevalence of wheeze symptom,diagnosed asthma,nasal symptoms and allergic rhinitis of Guangzhou was significantly higher than that of Conghua (P<0.0001),with 28.6%,27.7%,66.0%,46.4% for Guangzhou respectively,and 5.0%,2.5%,31.0%,6.2% for Conghua respectively.The positive rates of bronchial provocation test and skin sensitization against Dermatophagoides pteronyssinus(Der.p),D.farinae (Der.f),cat and dog allergen in the students of Guangzhou were significantly higher than that of Conghua (P<0.0001).The positive rate of cockroach of Conghua was significantly higher than that of Guangzhou (P<0.0001).The levels of endotoxin (amount,concentration and load) of samples from Conghua were significantly higher than that from Guangzhou (Conghua:6452 EU,10.95 EU/mg,1794 EU/m2 ;Guangzhou:1591 EU,6.45 EU/mg,508.80 EU/m2,respectively).PD20-FEV1 was correlated positively with endotoxin amount(r=0.174,P<0.05).The diameters of the skin wheal size for Der.p and Der.f were correlated inversely with endotoxin amount and load (P<0.001).Conclusion The prevalence of wheeze symptom,diagnosed asthma,nasal symptoms and allergic rhinitis in 13-14 year-old adolescent of Guangzhou

  15. 抗粉尘螨主要变应原Der fⅡ单克隆抗体的制备与鉴定%Preparation and identification of monoclonal antibodies against Der f Ⅱ allergen

    Institute of Scientific and Technical Information of China (English)

    刘晓宇; 吉坤美; 李荔; 邹菊; 刘志刚

    2011-01-01

    目的 制备并鉴定鼠源抗粉尘螨主要变应原Der fⅡ单克隆抗体(Monoclonal Antibody,McAb).方法 重组Der fⅡ蛋白为抗原免疫BALB/c小鼠,取小鼠免疫脾细胞与NS-1细胞融合.间接ELISA法筛选特异性分泌的杂交瘤细胞.用筛选获得的单克隆细胞株诱生小鼠腹水,蛋白G亲和层析法纯化腹水抗体.利用Ig类与亚类鉴定试剂盒鉴定该单克隆抗体的Ig亚型;通过间接ELISA、Western Blotting方法鉴定该单克隆抗体的特性和交叉性.结果 获得5株IgG2a型鼠抗粉尘螨主要变应原Der fⅡ的单克隆抗体,效价良好.ELISA和Western Blotting分析表明该5株单抗均可识别重组Der fⅡ蛋白和天然粉尘螨提取物.结论 成功制备了5株鼠抗粉尘螨主要变应原Der fⅡ的单克隆抗体,为建立粉尘螨主要变应原Der fⅡ的检测及纯化方法奠定了基础.%In order to prepare monoclonal antibodies from mice against Der f II allergen from Dermatophagoid.es farinae and to characterize their properties, BALB/c mice were immunized with purified recombinant Der f II allergen protein, and the splenocytes of the immunized mice were fused with NS-1 myeloma cells by hybridoma technique. Indirect ELISA was used for screening the hybridoma cell lines which could secrete antibodies against Der f II. The McAbs against Der f fl allergen were purified using affinity chromatography on immobilized Protein G to identified their specificity, subtype, titers and cross-reactvity by ELISA and Western blotting. Five hybridoma cell lines secreting McAbs against Der f fl allergen were obtained, which were determined as IgG2a subtype and good ascites titers. The five McAbs against Der f II allergen all recognized recombinant Der f II allergen and native extract from Dermatophagoides farinae. Five monoclonal antibodies against Der f II allergen were prepared successfully, which will facilitate establishing detection method of Der Ⅱ allergen.

  16. Risk factors of acarines sensitization for respiratory allergic diseases of children in Guangzhou urban districts%广州地区儿童呼吸道变态反应性疾病螨性变应原相关危险因素分析

    Institute of Scientific and Technical Information of China (English)

    陈德晖; 钟南山; 江梅; 李靖; 孙宝清; 林育能; 潘小安; 钟帼钰; 陈庆宜; 陈福雄

    2010-01-01

    见的变应原是屋尘螨、粉尘螨及热带螨,可以通过SPT检查明确过敏原.年龄增加、反复鼻炎、眼部、哮喘症状控制不良是螨类致敏呼吸道变态反应性疾病发病的危险因素.变态反应性疾病应以早期防治为医疗工作的重要方针,研究本地区变态反应性疾病患者的变应原种类特点及危险因素,有助于疾病的早期诊断和早期防治,并可针对变应原采取特异性免疫治疗,可望使螨性哮喘和鼻炎患儿达到并维持稳定.%Objective To evaluate the common allergies and the acarines sensitization as risk factors for respiratory allergic diseases of children in Guangzhou urban districts and to provide the evidence for preventing allergic diseases. Methods All cases were collected from pediatric out-patient department of the first affiliated hospital of Guangzhou medical college from february lst,2006 to march 30th,2007. One hundred and eighty three children were above 5 years old, were diagnosed asthma and (or) allergic rhinitis,and lived in Guangzhou urban districts. In 183 cases, 132/183 were males and 51/183 were females. The average age was (8. 2 ± 0.2) years old in males and ( 7.8 ± 0.44) years old in females.Among 183 patients,58 cases were asthma,20 cases were rhinitis and 105 cases were asthma and rhinitis.All the patients of their parents or guardians completed a standardized questionnaire on the presence of respiratory and allergic symptoms. They also underwent skin prick tests with 13 common aeroallergens,total IgE,specific IgE and eosinophil count in serum. Results Among the 183 patients, 157 (85.8%) had at least one positive skin reaction. The rates of positive skin reaction with 13 common aeroallergens were from 5.5% to 75.4%. The positive rate of Dermatophagoides pteronyssinus(Der p) was the highest (79.8% ,146/183), then followed up by Dermatophagoides farinae(Der f) (72.7%, 133/183), Blomia tropicalis (65.0%, 119/183), dog(48.6 % ,89/183), American

  17. Infection by Ascaris lumbricoides and bronchial hyper reactivity: an outstanding association in Venezuelan school children from endemic areas.

    Science.gov (United States)

    Hagel, I; Cabrera, M; Hurtado, M A; Sanchez, P; Puccio, F; Di Prisco, M C; Palenque, M

    2007-09-01

    Asthma and other respiratory diseases have increased in the last years among Venezuelan children from helminthic endemic areas where the infection by Ascaris lumbricoides has been associated to bronchial airway inflammation in parasitized individuals. The aim of this work was to investigate the possible associations between the development of bronchial hyper reactivity and the immune response against A. lumbricoides in urban and rural children. We evaluated 470 school children from rural and urban communities. Pulmonary function tests were performed and >or=20% PC(20) changes were considered as a positive diagnostic of bronchial hyper reactivity. The prevalence and intensity of A. lumbricoides infection was determined by faecal examination. Specific serum IgE levels using a modified ELISA and skin prick tests against A. lumbricoides and the common allergen Dermatophagoides pteronyssinus were done. The number of circulating lymphocyte sub populations was determined by flow cytometry analysis. In rural children, bronchial hyper reactivity was associated with increased specific levels of anti-A. lumbricoides IgE (p<0.0001) and skin test positivity for A. lumbricoides (p<0.0001). The percentage of FEV1 predictive values correlated inversely (p<0.0001) with anti-A. lumbricoides IgE levels. Elevated numbers of circulating CD3+CD4+ and CD20+CD23+ cells were found in rural children with bronchial hyper reactivity compared to their asymptomatic counterparts. They correlated positively with anti-A. lumbricoides IgE levels (p<0.005 and <0.0001, respectively). In contrast, in urban children, bronchial hyper reactivity was associated with elevated anti-D. pteronyssinus IgE levels (p=0. 0089), skin hyper reactivity towards this aero allergen (p=0.003) and to an increase in the number of CD3+CD8+ (p<0.0001). Our results suggest that the IgE response against A. lumbricoides infection may be involved in the development of bronchial hyper reactivity among rural children from endemic

  18. Are cutaneous hypersensitivity tests to inhalant allergens a severity marker for vernal keratoconjunctivitis? Os testes de hipersensibilidade cutânea contra alérgenos inalantes são marcadores de gravidade da ceratoconjuntivite vernal?

    Directory of Open Access Journals (Sweden)

    Lauro Augusto de Oliveira

    2007-12-01

    Full Text Available PURPOSE: The purpose of this study was to analyze the cutaneous sensitivity to a variety of allergens in patients with vernal keratoconjunctivitis (VKC and to demonstrate the relation between skin response and clinical aspects of the disease. METHODS: Twenty patients with vernal keratoconjunctivitis were randomly chosen from the External Disease and Cornea Sector. They were clinically evaluated, and a score ranging from 0 to 20 was applied based on signs and symptoms on ophthalmic examination. All subjects underwent a skin prick test against standardized allergens, such as house dust mites D. pteronyssinus, D. farinae, and Blomia tropicalis, as well as allergens from cat, dog, fungi and feather. RESULTS: Seventy-five per cent of patients were positive for at least one of the allergens tested. House dust mites were responsible for the majority of the cases (75%. There was a poor correlation between the clinical score and sensitivity to allergens (r= - 0.119 for fungi; r= - 0.174 for dog; r= - 0.243 for house dust mites; r= - 0.090 for feather. A significant correlation was found only for cat allergen extract (r = - 0.510; p=0.024. CONCLUSIONS: Our study demonstrated poor correlation between cutaneous hypersensitivity tests and clinical findings in patients with vernal keratoconjuntivitis. We concluded that skin response to inhalant allergens is not a useful test to identify clinical severity and chronicity of inflammatory process in this disease.OBJETIVO: Avaliar o papel da sensibilização cutânea a diferentes aeroalérgenos em pacientes com ceratoconjuntivite vernal e a correlação entre esta e os aspectos clínicos da doença. MÉTODOS: Vinte pacientes do setor de doenças externas e córnea foram aleatoriamente convidados para participar deste estudo. Os pacientes foram avaliados e a eles foi atribuído um escore clínico variando de 0 a 20 de acordo com sinais e sintomas presentes no exame oftalmológico. Todos os pacientes foram

  19. Effects of the recombinant allergen rDer f 2 on neuro-endocrino-immune network in asthmatic mice.

    Science.gov (United States)

    Jiang, Yong-Qian; Zhou, Zhi-Xiang; Ji, You-Lin

    2014-01-01

    Severe and life-threatening side effects can occur in patients receiving allergen-specific immunotherapy (SIT), and recombinant allergens made from cDNA have been used in clinical trials for ten years and appear promising for SIT. The aim of this study is to explore the effects of the recombinant allergen Der f 2 (the group 2 allergen from Dermatophagoides farinae) on the neuro-endocrino-immune network in asthmatic mice. Twenty-eight mice were divided into four groups - A, B, C and D. To induce asthma, a crude extract of D. farinae was injected intraperitoneally into the mice in groups B, C and D. Later, the crude extract or recombinant allergen rDer f 2 was given to groups C and D, respectively. Normal saline was given to groups A and B. Serum corticotropin-releasing hormone (CRH), adrenocorticotropic hormone (ACTH), corticosterone (CORT), interleukin 4 (IL-4), and interferon γ (IFN-γ) were detected by immunoassay and the pathological change of lung tissue was observed by hematoxylin and eosin (HE) staining. Serum CRH, ACTH, CORT, and IFN-γ were highest in healthy group A but lowest in asthma group B. Treatment with the crude extract or recombinant allergen rDer f 2 significantly attenuated this response in asthmatic mice, but there was no difference between the two treatments (p > 0.05). Serum IL-4 was elevated in asthma group B but lowest in healthy group A. Treatment with the crude extract or recombinant allergen rDer f 2 significantly attenuated this response in asthmatic mice, but there was no significant difference between the two treatments (p > 0.05). However, lung pathology as measured histologically (Underwood Score) showed that rDer f 2-treatment was significantly better than crude extract treatment (p < 0.05). In brief, recombinant allergen Der f 2 can strengthen the function of hypothalamus-pituitary-adrenal (HPA) axis, affect the balance of Th1 and Th2 cytokines, and reduce pulmonary inflammation in asthmatic mice.

  20. 485 Therapeutic Effect and Safety of Tropical Mite Allergen Vaccines by Subcutaneous Route in Allergic Asthmatics Patients

    Science.gov (United States)

    Castro Almarales, Raúl Lázaro; Ronquillo, Mercedes; Castello, Mirta Alvarez; Rodríguez, José; González, Mayda; Labrada, Alexis; Navarro Viltres, Bárbara I; Díaz, Yunia Oliva; Mateo, Maytee

    2012-01-01

    Background Allergen-specific subcutaneous immunotherapy (SCIT) is presently recognized as a biological response modifier, as it is the only available treatment able to influence the natural course of allergic disease. Extensive clinical evidence supports its efficacy. Safety concerns are related to the risks of anaphylactic reactions during treatment. Standardization of allergen vaccines in terms of allergenic activity allows a more precise control over the administered doses and can be, therefore, very relevant for both efficacy and safety of SCIT. House Dust Mites (HDM), particularly Dermatophagoides pteronyssinus (Dp), Dermatophagoides siboney (Ds) and Blomia tropicalis (Bt) have been described as very relevant allergen sources in Cuba, with a strong association to respiratory allergy symptoms. Objective To asses the efficacy and safety of standardized allergen vaccines of these 3 mite species (Valergen, Biocen, Cuba) in Cuban asthmatic patients. Methods Three Double-Blind Placebo-Controlled clinical trials were performed in 40 patients each, showing asthmatic symptoms and positive predominant Skin Prick Test (SPT) to each mite, respectively. Half of patients received the active treatment consisting of subcutaneous injections with increasing doses, up to 6000 BU. Results The total 1 year cumulative dose was 63035 BU, in an average of 20.5 injections. The treatment was effective in the reduction of clinical symptoms (up to 32%, 95%CI: 28-36%; P = 0.0006) and medication intake (23%, 95%CI:18-28%), as compared to control treatment. The skin sensitivity to the allergens decreased significantly (P = 0.0001), with regard to the beginning of the treatment. The allergen amount needed to induce a positive SPT increased 297-fold. An improvement of the lung function was observed, expressed in a modest Peak-Expiratory-Flow increase (P < 0.05) and reduction of PEF daily variability. SIT was considered effective in 71% of patients. The frequency of local adverse reactions was

  1. Ready-to-use house dust mites atopy patch test (HDM-Diallertest), a new screening tool for detection of house dust mites allergy in children.

    Science.gov (United States)

    Benhamou, P H; Kalach, N; Soulaines, P; Donne, N; Dupont, C

    2009-10-01

    to assess the accuracy and safety of a ready-to-use atopy patch test (HDM-Diallertest, DBV Technologies, Paris) in the diagnosis of sensitization to house-dust mite (HDM) allergens in children with or without atopic dermatitis. prospective analysis of a systematic allergic work-up was carried out in 47 children, age 57.4+42 months (mean + SD, range 7 to 176 mo), presenting with isolated or combined atopic dermatitis (AD, n = 28) or other symptoms without AD (control group, n = 19). Children were routinely tested for specific HDM-IgE [against D. pteronyssinus (DPT) and D. farinae (DF)], and skin testing based on HDM (DPT & DF) skin prick test (SPT) and ready-to-use HDM-ATP (HDM-Diallertest), with a reading at 72 hours. 15 children (31.9%) exhibited specific IgE against both DPT and DF, 16 children (34.04%) exhibited positive SPT against DPT and 17 (36.1%) against DF. HDM-Diallertest was positive in 15 cases (31.9%). Among these, 9 exhibited with an eczematous reaction showed an excellent correlation with both SPT and specific IgE for DPT and DF, respectively 93.3%, 97.77%, 90.47%, and 90.47%. The different diagnostic techniques of HDM sensitization neither differ between groups, nor correlated specifically with the different clinical manifestations. No side effect was observed during and after patch testing, except for a local reaction without diffusion outside the local test area. The 3 diagnostic techniques exhibited a comparable level of accuracy for the diagnosis of HDM allergens sensitization. The excellent concordance of the highest class reactions of HDM-Diallertest with the other diagnostic techniques indicates a potential role as a screening tool for the detection of HDM sensitization in infancy.

  2. Sensitization to neumoallergens influence in mothers of asthmatic children.

    Science.gov (United States)

    Sánchez Palacios, A; Schamann, F; García, J A; Sánchez, M A; Rodríguez, A

    1996-01-01

    Allergy diseases in our province have the characteristic of early appearance of asthma and predominant monosensitization to domestic dust mites due to the climatic condition. In this work we study the influence in allergic children. We selected 1028 mothers that brought their children to hospital for the first time. We divided them in two groups. One was comprised of 459 mothers of atopic children (MA) and another of 569 mothers of non atopic children (MC). All mothers were skin-prick tested (Phazet). The papula area, the mothers with aeroallergen sensitization, were 180 (17.5%) between MA 126 (27.4%) and MC 54 (9.5%). The diagnosis of allergic mothers was higher in MA than in MC. Asthma 8.39%, Rhinitis 4.75% and dermatitis 2%. 53.31% lived on the coast. 51.45% had house moulds. 31% had animals. 34.9% were smokers. There were more adverse drug reactions in MA than in MC. 180 mothers presented sensitization. D. pteronyssinus 66.3%, D. farinae 65.5% Lolium P. 14.55%, Cats 11.6% (MA > MC), Artemisia Vulgaris 6.25% and Cladosporium Herbarum 0.6%. There was no correlation between skin sensitization and domestic animals. There was no influence between children's IgE and mothers with skin sensitization. Skin sensitization to grass pollen correlated with the mother who was living in another country. The mothers who have been living more than five years in our province presented more skin sensitization to aero-allergens.

  3. IgE antibodies and urinary trimethylarsine oxide accounted for 1-7% population attributable risks for eczema in adults: USA NHANES 2005-2006.

    Science.gov (United States)

    Shiue, Ivy

    2015-12-01

    Population attributable risks from serum IgE and dust miteallergen concentrations and environmental chemicals for eczema are unclear. Therefore, it was aimed to examine serum IgE and allergen concentrations and environmental chemicals for eczema in adults and to calculate population attributable risks in a national and population-based setting. Data retrieved from the National Health and Nutrition Examination Survey, 2005-2006, was analyzed. Information on demographics and self-reported ever eczema was obtained by household interview. Bloods and urines (sub-sample) were also collected during the interview. Adults aged 20-85 were included. Statistical analyses were using chi-square test, t test, survey-weighted logistic regression modeling, and population attributable risk (PAR) estimation. Of all the included American adults (n = 4979), 310 (6.2%) reported ever eczema. Moreover, more eczema cases were observed in female adults but fewer cases in people born in Mexico. There were no significant associations observed between commonly known biomarkers (including vitamin D) and eczema or between dust mite allergens and eczema. Serum D. Farinae (PAR 1.0%), D. Pteronyssinus (PAR 1.1%), cat (PAR 1.8%), dog (PAR 1.6%), and muse (PAR 3.2%) IgE antibodies were associated with eczema. Adults with ever eczema were found to have higher levels of urinary trimethylarsine oxide concentrations (PAR 7.0%) but not other speciated arsenic concentrations. There were no clear associations between other environmental chemicals including heavy metals, phthalates, phenols, parabens, pesticides, nitrate, perchlorate, polycyclic hydrocarbons and eczema as well. Elimination of environmental risks might help delay or stop eczema up to 7% in the adult population.

  4. 不同年龄段变应性疾病患者变应原检测分析%Analysis of Different Ages in Patients with Allergic Diseases Allergen Detection

    Institute of Scientific and Technical Information of China (English)

    魏峻

    2014-01-01

    Objective Al ergen detection in ptients with al ergic disease using serologicl detection method,analzing and comparing by age group. Methods For patients in our hospital during November 2012 to July a total 535 al ergic reaction,slgE serological detection of al ergen by Al ergyScreen system manufactured by Mediwess company in Germnay. Results ①Children al ergen positive rate was 73.14%,higher than the other age.②Distributions of al ergens of dif erent ages have dif erent,inhaled al ergens,the positive rate of slgE was highest in Dermatophagoides pteronyssinus 78.5%,children dermatophagoides pteronyssinus lgE positive rate was higher than the other age groups,89.74%. ③For inhalation al ergen detection of dif erent ages, the values of slgE are significantly dif erent for house dust mite and cat dog dander group, detection of house dust mite slgE values,different groups of children and other age is the most obvious(=2.018, 3.174, 3.476;=0.045,0.002, 0.001).For food al ergens in children group for milk,and the other three groups were significnt dif erences (=2.163, 2.338, 2.370, p=0.026,0.021,0.02). Conclusion Distribution of positive rate in different al ergens and the positive rate of the overal change with age is not consistent.At the same time,slgE detection value differences of children group and other groups also has the largest.Recommended intervention on early al ergic diseases of children.%目的采用体外血清学检测方法检测变应性疾病患者的变应原,并按年龄段分组进行比较分析。方法对2012年7月~11月我院门诊就诊的变态反应患者共535例,采用德国Mediwess公司生产的Al ergy Screen系统进行变应原的slgE血清学检测。结果①儿童组变应原检出阳性率为73.14%,高于其他各年龄段;于不同年龄段的变应原分布有差异,吸入性变应原中,slgE阳性率最高者为户尘螨,占78.5%;儿童组户尘螨lgE阳性率又高于其他各年龄段,为89.74%;③对

  5. Inmunoterapia en el tratamiento de la conjuntivitis alérgica crónica Immunotherapy in the treatment of chronic allergic conjuntivitis

    Directory of Open Access Journals (Sweden)

    Fernando Montoya Maya

    1996-02-01

    Full Text Available

    Con el objeto de evaluar la respuesta a dos esquemas de Inmunoterapia alergénica para el tratamiento de la conjuntivitis alérgica crónica (CAC en Individuos sensibles a Dermatophagoides farinae (ácaro del polvo y refractarios a los tratamientos convencionales, se realizó un ensayo clínico controlado y ciego en el Hospital Universitario San Vicente de Paúl de Medellín, Colombia, entre agosto de 1990 y junio de 1993. Se estudiaron 18 hombres (82% y 4 (18% mujeres con edades entre los 5 y 25 años pero en su mayoría menores de 11 años (63.6%; la evolución de su enfermedad era mayor de 5 meses. Se excluyeron los pacientes que habían recibido previamente algún esquema de Inmunoterapia o que eran sensibles a otros aeroalergenos. En 14 pacientes (63.6% se administró el esquema agrupado (tipo cluster y en ocho (36.4% el convencional. No se encontraron diferencias estadísticamente significativas entre las dos modalidades; en 16 pacientes (72.7% hubo mejoría subjetiva parcial, demostrada por el interrogatorio, y en 15 (68.2% mejoría objetiva parcial evidenciada por el examen oftalmológico. En tres pacientes de tratamiento agrupado no se obtuvo mejoría. Los síntomas que más disminuyeron al finalizar el seguimiento fueron el prurito en 16 casos (72.7% y ellagrimeo (15 casos; 68.2%; sólo en 4 de los 11 pacientes (36.4% que al Inicio del estudio tenían papllas se observó la desaparición de éstas, a pesar de una buena respuesta clínica. Se concluyó que la inmunoterapla es útil en el manejo de la CAC refractaria a tratamientos convencionales y no se encontraron diferencias significativas entre los dos esquemas utilizados.

    Between August 1990 and June 1993, a blind clinic assay was carried out at Hospital Universitario San Vicente de Paúl, in

  6. 成都地区过敏性鼻炎患者过敏原的检测分析%Detection and analysis of allergens in allergic rhinitis patients in Chengdu

    Institute of Scientific and Technical Information of China (English)

    蒋锐明; 范敏; 谢亚柳; 袁洛花; 许琴

    2013-01-01

    目的 检测成都地区过敏性鼻炎(AR)患者血清中的过敏原情况,为临床诊断和治疗过敏性鼻炎提供实验室依据.方法 过敏性鼻炎患者88例及同期就诊的非过敏性鼻炎患者30例,采用酶免疫斑点法,检测患者血清中的吸入性和食入性过敏原共20项.结果 吸入性过敏原前3位为:屋尘螨/粉尘螨组合(65.9%)、屋尘(43.2%)狗上皮(9.1%);食入性过敏原前3位为:虾和海鱼组合(13.6%)、艾蒿(11.4%)、黄豆(10.2%).30例阴性对照仅有1例检测出阳性过敏原为虾和海鱼.结论 屋尘螨/粉尘螨和虾/海鱼分别是导致成都地区AR最主要的吸入性和食入性过敏原,有意识地避免接触这些过敏原及进行特异性的免疫治疗对防治过敏性鼻炎具有积极意义.%Objective To detect allergen in serum of patients with allergic rhinitis (AR) for diagnosis and treatment.Methods Sera from 88 AR patients and 30 non-allergic rhinitis controls were collected and detected for 20 kinds of allergen by using enzyme-linked immunospot assay.Results In Chengdu city,the positve rates arrangement of top 3 of inhalant allergens are house dust mite/dermatophagoides farinae (65.9%),house dust (43.2%) and dog epithelium (9.1%),respectively and of ingested allergens are shrimp/marine fish (13.6%),artemisia argyi (11.4%) and soybean (10.2%),respectively.Only 1 of 30 controls is allergic to shrimp and marine fish.Conclusion House dust mite/dermatophagoides farinae and shrimp/marine fish are the uppermost inhalant and ingested allergens recpectively which induced AR in Chengdu city.Dection of allergen is meaningful for patients to prevent suffering from AR disease by avoiding touching these allergens consciously,and it is important for clinicians to perform specific immunotherapy on AR patients.

  7. Tropomyosin IgE-positive results are a good predictor of shrimp allergy.

    Science.gov (United States)

    Gámez, C; Sánchez-García, S; Ibáñez, M D; López, R; Aguado, E; López, E; Sastre, B; Sastre, J; del Pozo, V

    2011-10-01

    Shrimp is a common cause of food allergy. Our aims were to determine the value of IgE antibodies in the diagnosis of shrimp allergy and to study red shrimp (Solenocera melantho) tropomyosin both as a new allergen and as a cross-reactive IgE-binding protein. We have studied 45 subjects. Skin prick test (SPT) was carried out in all subjects, and specific IgE (sIgE) to shrimp, recombinant and natural shrimp tropomyosins rPen a 1 and nPen m 1, recombinant Der p 10, and Dermatophagoides pteronyssinus was assessed by fluoroimmunoassay and/or immunoblotting. Double-blind, placebo-controlled food challenges were carried out to confirm diagnosis of shrimp allergy. Also, in vitro inhibition tests were performed to evaluate cross-reactivity. Shrimp allergy was confirmed in 18 shrimp-allergic patients. Skin prick test and IgE antibodies to shrimp were positive in all shrimp-allergic patients; sIgE to rPen a 1 was detected in 98% of these patients. Of the 18 shrimp-tolerant patients, 61% had positive SPT to shrimp, 55% were IgE-positive to shrimp, and 33% showed IgE antibodies to rPen a 1. Determination of IgE to rPen a 1 yielded a positive predictive value of 0.72 and a negative predictive value of 0.91. IgE levels to rPen a 1 provided additional value to the diagnosis of shrimp allergy. Some allergens in mite extract are recognized by patients who are allergic to shrimp, though their clinical relevance remains unknown. © 2011 John Wiley & Sons A/S.

  8. Real-Life Study for the Diagnosis of House Dust Mite Allergy - The Value of Recombinant Allergen-Based IgE Serology.

    Science.gov (United States)

    Becker, Sven; Schlederer, Thomas; Kramer, Matthias F; Haack, Mareike; Vrtala, Susanne; Resch, Yvonne; Lupinek, Christian; Valenta, Rudolf; Gröger, Moritz

    2016-01-01

    Dermatophagoides pteronyssinus is one of the most important perennial allergen sources worldwide. Molecular diagnostics using the commercially available major allergens (Der p 1 and Der p 2) in combination with Der p 10 do not detect house dust mite (HDM) sensitization in a number of cases when used alone. The objective was to evaluate the IgE reactivity profiles of these patients using an experimental immunoassay biochip. Sera of HDM-allergic patients (positive skin prick test, CAP class ≥1 for allergen extract, and positive intranasal provocation) were tested for IgE antibodies against Der p 1, Der p 2, and Der p 10 by ImmunoCAP fluorescence enzyme immunoassay. Negatively tested sera were examined by an experimental chip containing 13 microarrayed HDM allergens. Of 97 patients tested, 16 showed negative results to Der p 1, Der p 2, and Der p 10. MeDALL chip evaluation revealed 5 patients monosensitized to Der p 23, and 11 patients were negative for all HDM MeDALL chip components. Seven sera were available for further testing, and 3 of them showed IgE reactivity to dot-blotted nDer p 1, and 2 reacted with high-molecular weight components (>100 kDa) in nitrocellulose-blotted HDM extract when tested with 125I-labeled anti-IgE in a RAST-based assay. The HDM extract-specific IgE levels of the 11 patients were <3.9 kU/l. Recombinant allergen-based IgE serology is of great value when conventional IgE diagnostics fails. Der p 23 is an important HDM allergen, especially when major allergens are negative. Therefore, it would be desirable to have Der p 23 commercially available. Further research concerning the prevalence and clinical significance of different HDM allergens is needed. © 2016 S. Karger AG, Basel.

  9. IgE Abs to Der p 1 and Der p 2 as diagnostic markers of house dust mite allergy as defined by a bronchoprovocation test.

    Science.gov (United States)

    Minami, Takafumi; Fukutomi, Yuma; Lidholm, Jonas; Yasueda, Hiroshi; Saito, Akemi; Sekiya, Kiyoshi; Tsuburai, Takahiro; Maeda, Yuji; Mori, Akio; Taniguchi, Masami; Hasegawa, Maki; Akiyama, Kazuo

    2015-01-01

    Limited information is available regarding the clinical usefulness of measuring the levels of IgE to allergen components from house dust mites (HDMs) in the diagnosis of genuine HDM allergy. To evaluate the diagnostic usefulness of measuring levels of serum IgE antibodies (Abs) to allergen components from Dermatophagoides pteronyssinus (DP) as a predictor of immediate asthmatic response (IAR) to bronchoprovocation, we studied 55 DP-sensitized asthmatic patients who underwent a bronchoprovocation test using crude DP extract. The levels of IgE Abs to crude DP, nDer p 1, rDer p 2, and rDer p 10 in patients who showed IAR (n = 41) were compared with those in patients who showed no IAR (n = 14). While the frequencies of positivity for IgE Abs to nDer p 1 and rDer p 2 among the entire study population were 89 and 86%, respectively, all patients with IAR tested positive for both of them with high IgE concentrations. The areas under the receiver operating characteristic curves for IgE to nDer p 1 and rDer p 2 as predictors of IAR were 0.913 and 0.906, respectively. The specificity of IgE to nDer p 1 and rDer p 2 was higher than IgE to crude DP even at low cut-off points. IgE to nDer p 1 and/or rDer p 2 was highly predictive of allergen-induced IAR. These findings validate the clinical usefulness of measuring the levels of IgE to nDer p 1 and rDer p 2 as a diagnostic tool for genuine HDM allergy. Copyright © 2014 Japanese Society of Allergology. Production and hosting by Elsevier B.V. All rights reserved.

  10. DNA vaccine encoding Der p2 allergen down-regulates STAT6 expression in mouse model of allergen-induced allergic airway inflammation

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    Background Activation of signal transducer and activator of transcription 6 (STAT6 ) plays a critical role in the late phase of Th2-dependent allergy induction. STAT6 is essential to Th2 cell differentiation, recruitment, and effector function. Our previous study confirmed that DNA vaccination inhibited STAT6 expression of spleen cells induced by allergen. In the present study, we determined whether DNA vaccine encoding Dermatophagoides pteronyssinus group 2 (Der p2 ) could down-regulate the expression and activation of STAT6 in lung tissue from asthmatic mice.Methods After DNA vaccine immunization, BALB/c mice were sensitized by intraperitoneal injection and challenged by intranasal instillation of rDer p2. The levels of the cytokines IL-4 and IL-13 in BAL fluid were measured by enzyme-linked immunosorbent assay. The lung tissue was assessed by immunohistochemical staining with anti-STAT6. The protein expression of STAT6 was determined by Western blot. The activation of STAT6 binding ability was analyzed with electrophoretic mobility shift assay.Results DNA vaccine encoding Der p2 allergen effectively decreased the levels of IL-4 and IL-13 in the asthmatic mice. Histological evidence and Western blot showed that the expression of STAT6 in the DNA treated mice was markedly attenuated. STAT6 binding to specific DNA motif in lung tissue from the gene vaccinated mice was inhibited.Conclusion DNA vaccine encoding Der p2 prevents allergic pulmonary inflammation probably by inhibiting the STAT6 signaling pathway in mice with Der p2 allergen-induced allergic airway inflammation.

  11. Impairment of T-regulatory cells in cord blood of atopic mothers.

    Science.gov (United States)

    Schaub, Bianca; Liu, Jing; Höppler, Sabine; Haug, Severine; Sattler, Christine; Lluis, Anna; Illi, Sabina; von Mutius, Erika

    2008-06-01

    Maternal atopy is a strong predictor for the development of childhood allergic diseases. The underlying mechanisms are ill defined, yet regulatory T (Treg) and T(H)17 cells may play a key role potentially shaping the early immune system toward a proallergic or antiallergic immune regulation. We examined T(H)1/T(H)2, Treg, and T(H)17 cell responses to innate (lipid A/peptidoglycan) and mitogen/adaptive (phytohemagglutinin/Dermatophagoides pteronyssinus 1) immune stimulation in cord blood from offspring of atopic/nonatopic mothers. Cord blood mononuclear cells from 161 healthy neonates (59% nonatopic, 41% atopic mothers) were investigated regarding Treg and T(H)17 cells (mRNA/surface markers), suppressive function, and proliferation/cytokine secretion. Cord blood from offspring of atopic mothers showed fewer innate-induced Treg cells (CD4(+)CD25(+)high), lower mRNA expression of associated markers (glucocorticoid-induced tumor necrosis factor receptor-related protein/lymphocyte activation gene 3; P cell function was impaired in mitogen-induced suppression of T effector cells in cord blood of offspring from atopic mothers (P = .03). Furthermore, IL-10 and IFN-gamma secretion were decreased in innate-stimulated cord blood of offspring from atopic mothers (P = .04/.05). Innate-induced IL-17 was independent of maternal atopy and highly correlated with IL-13 secretion. In offspring of atopic mothers, Treg cell numbers, expression, and function were impaired at birth. T(H)17 cells were correlated with T(H)2 cells, independently of maternal atopy.

  12. Detection of allergenic compounds using an IL-4/luciferase/CNS-1 transgenic mice model.

    Science.gov (United States)

    Bae, Chang Joon; Lee, Jae Won; Bae, Hee Sook; Shim, Sun Bo; Jee, Seung Wan; Lee, Su Hae; Lee, Chang Kyu; Hong, Jin Tae; Hwang, Dae Youn

    2011-04-01

    The interleukin-4 (IL-4) signaling cascade has been identified as a potentially important pathway in the development of allergies. The principal objective of this study was to produce novel transgenic (Tg) mice harboring the luciferase gene under the control of the human IL-4 promoter and the enhancer of IL-4 (CNS-1), in an effort to evaluate three types of allergens including a respiratory sensitizer, vaccine additives, and crude extracts of natural allergens in vivo. A new lineage of Tg mice was generated by the microinjection of pIL-4/Luc/CNS-1 constructs into a fertilized mice egg. The luciferase activity was successfully regulated by the IL-4 promoter in splenocytes cultured from IL-4/Luc/CNS-1 Tg mice. From the first five founder lines, one (#57) evidencing a profound response to ovalbumin was selected for use in evaluating the allergens. Additionally, the lungs, thymus, and lymph nodes of IL-4/Luc/CNS-1 Tg mice evidenced high luciferase activity in response to allergens such as phthalic anhydride (PA), trimellitic anhydride, ovalbumin, gelatin, Dermatophagoides pteronyssinus extracts, and Japanese cedar pollen, whereas key allergy-related indicators including ear thickness, Immunoglobulin E concentration, and the infiltration of inflammatory leukocytes in response to PA were unaltered in the Tg mice relative to the non-Tg mice. Furthermore, the expression levels of endogenous type 2 helper T cells cytokines and proinflammatory cytokines were similarly increased in these organs of IL-4/Luc/CNS-1 Tg mice in response to allergens. These results indicate that IL-4/Luc/CNS-1 Tg mice may be used as an animal model for the evaluation and prediction of the human body response to a variety of allergens originating from the environment and from certain industrial products.

  13. Prevalence of sensitivity to cockroach allergens and IgE crossreactivity between cockroach and house dust mite allergens in Chinese patients with allergic rhinitis and asthma

    Institute of Scientific and Technical Information of China (English)

    SUN Bao-qing; LAI Xu-xin; Birgitte Gjesing; Michael Dho Spangfort; ZHONG Nan-shan

    2010-01-01

    Background Cockroaches are an important indoor allergen source causing allergic rhinitis and asthma. The aim of this study was to investigate the cockroach prevalence in mainland of China and the cross-reactivity of IgE between cockroach and house dust mite allergen in Chinese patients.Methods The cockroach sensitization pattern was based on a skin prick test (SPT) obtained from a national multicenter prevalence study, in which 6304 patients from 25 allergy centers across China participated. Factors, including different regions of China, age, gender and the correlations between the American and German cockroaches and house dust mite Der p were investigated. Eighteen out of 1236 clinical sera from south China were selected to perform the cross-inhibition assay between house dust mites and cockroaches.Results Totally 25.7% of patients were SPT positive to the American cockroach (Periplaneta Americana, Per a) and 18.7% SPT positive to the German cockroach (Blattella germanica, Bla g). The prevalence of positive cockroach SPT was higher in southern than in northern China, higher in adults than in children, and higher in males than in females.Patients had relatively low levels of cockroach SPT reactions, mainly class 1 or 2. Of the SPT positive cockroach patients,88% were also SPT positive to house dust mite (Dermatophagoides pteronyssinus, Der p). An IgE cross-inhibition study confirmed that Der P sensitization could cause false positive SPT reactions against cockroach.Conclusions A relatively high prevalence of cockroach sensitivity was found in mainland of China. However, a cross-inhibition study showed that only a small number of patients appear to have Bla g and/or Per a as primary sensitizing source. The importance of cockroaches as a risk factor for sensitization and triggers of allergic symptoms in mainland of China needs to be further investigated.

  14. Prescripción y vigilancia de la inmunoterapia sublingual con vacunas estandarizadas de ácaros domésticos en un servicio de alergología

    Directory of Open Access Journals (Sweden)

    Mirta Álvarez

    2012-12-01

    Full Text Available Las vacunas terapéuticas para la alergia, también llamadas inmunoterapia alergeno específica, consisten en la administración de dosis progresivamente crecientes del alergeno al cual el individuo está sensibilizado, con el objetivo de alcanzar tolerancia al mismo y disminuir la sintomatología clínica. Se realizó un estudio descriptivo, de corte transversal para determinar la seguridad de las vacunas de ácaros domésticos (VALERGEN, administradas mediante gotas por vía sublingual. Se verificaron sus eventos adversos en pacientes alérgicos, atendidos en el Departamento de Alergología del hospital universitario "Calixto García", que asistieron a cambios de vacunas en el mes de septiembre de 2010, así como la frecuencia de prescripción de vacunas alergénicas en el período enero-septiembre del mismo año. Se incluyeron 130 pacientes con tratamiento de inmunoterapia sublingual con VALERGEN, con una edad media de 19,6 años (rango 1-75; el 40,7% tenía 17 años o menos. El tipo de vacuna más empleada resultó ser la multialergénica (63,8%. El ácaro más empleado fue el Dermatophagoides pteronyssinus, seguido de Blomia tropicalis . El 71,55% de los casos se encontraban en fase de mantenimiento. Se reportaron cuatro eventos adversos (3,1% locales leves, que no requirieron tratamiento ni cambio de pauta de vacunación. Se comprobó que las vacunas sublinguales VALERGEN son seguras y bien toleradas en los pacientes alérgicos.

  15. Clinical and immunological features of patients with atopy and concomitant HTLV-1 infection

    Directory of Open Access Journals (Sweden)

    F.P. Gaspar-Sobrinho

    2010-12-01

    Full Text Available Human T-cell lymphotropic virus type 1 (HTLV-1 induces an exacerbated type 1 immune response characterized by high spontaneous IFN-γ and TNF-α production. Allergic rhinitis and asthma are associated with the type 2 immune response, with elevated secretion of IL-4 and IL-5. The aim of this study was to characterize the immune response in atopic HTLV-1 carriers. The cytokine profile of atopic HTLV-1 carriers (N = 10; all females was compared with that of non-atopic HTLV-1 carriers (N = 14; 9 females and 5 males. Mean patient age of atopic and non-atopic groups was 45 ± 8 and 38 ± 11 years, respectively. All atopic HTLV-1 carriers had rhinitis with or without asthma and a skin prick test positive for Dermatophagoides pteronyssinus antigen 1 (Derp-1. There was no difference in cytokine levels between the two groups in unstimulated peripheral blood mononuclear cell cultures. In cultures stimulated with Derp-1, IFN-γ levels tended to be higher (P = 0.06 and IL-5 levels were higher (P = 0.02 in atopic HTLV-1 patients than in non-atopic subjects. In contrast, IL-10 was lower (P = 0.004 in atopic than in non-atopic HTLV-1-infected subjects. This study shows that HTLV-1 infection with an exaggerated type 1 immune response does not prevent atopy. In this case, the exacerbated type 1 and type 2 immune responses were due to a lack of IL-10 production, a cytokine that plays an important role in down-modulating type 1 and type 2 immune responses and in preventing the development of chronic inflammatory diseases.

  16. The determinants of dust mite allergen and its relationship to the prevalence of symptoms of asthma in the Asia-Pacific region.

    Science.gov (United States)

    Wickens, Kristin; de Bruyne, Jessie; Calvo, Mario; Choon-Kook, Sam; Jayaraj, G; Lai, Christopher K W; Lane, Juliette; Maheshwari, Rameshchandra; Mallol, Javier; Nishima, Sankei; Purdie, Gordon; Siebers, Rob; Sukumaran, Thevaruparambil; Trakultivakorn, Muthita; Crane, Julian

    2004-02-01

    The role that house dust mites play in the primary causation of asthma is controversial. Approximately thirty-six 10-yr-old children in each of 10 centres in the Asia-Pacific region participated. Researchers collected dust from mattresses and living room floors using standardized procedures. Der p1 and Der f1 were analysed using a double monoclonal antibody enzyme-linked immunosorbent assay. Geometric mean allergen levels were calculated for each centre. An ecological analysis was conducted to show the regression of the geometric mean allergen level, using the highest household level, against asthma symptom and severity prevalence data from the International Study of Asthma and Allergies in Childhood, Phase I. Among children aged 13-14 yr, the change in asthma symptom prevalence was associated with per unit change in Der p1 microg/g (1.08, 95% CI 0.10-2.06) and Der 1 microg/g (Der p1 + Der f1) (0.64, 95% CI 0.02-1.26). The change in having four or more attacks of asthma in the last 12 months was associated with per unit change in Der p 1 microg/g (0.29, 95% CI -0.02 to 0.60) and Der 1 microg/g (0.20, 95% CI 0.01-0.38). There was no effect for total Der p1 or Der f1 (total or microg/g). Among children aged 6-7 yr, neither allergen was related to symptoms or severity prevalence. While our findings suggest that Dermatophagoides pteronyssinus may have a role in the primary causation of asthma, the complexity of this association reinforces the need for prospective studies.

  17. Enzymatic activity in extracts of allergy-causing astigmatid mites.

    Science.gov (United States)

    Morgan, Marjorie S; Arlian, Larry G

    2006-11-01

    Many of the previously characterized allergens of house dust mites are known to be proteases, and this enzymatic activity is thought to contribute to their allergenicity. Other astigmatid mites, including stored-product mites and the ectoparasitic itch mite, Sarcoptes scabiei De Geer, are also known to be allergenic, but little or nothing is known about their enzymatic activities. The purpose of this study was to characterize the enzymatic activities present in extracts of the parasitic itch mite and from eight other species of free-living astigmatid mites. Extracts were prepared from one parasitic mite (S. scabiei), five stored-product mites (Chortoglyphus arcuatus (Troupeau), Lepidoglyphus destructor (Schrank), Blomia tropicalis Bronswijk, Cock, Oshima, Tyrophagus putrescentiae (Schrank), and Acarus siro L.), and three house dust mites [Dermatophagoidesfarinae Hughes, Dermatophagoides pteronyssinus (Troussart), and Euroglyphus maynei (Cooreman) ]. ApiZym strips were used to screen for the presence of 19 individual enzyme activities. Digestion of nine other substrates was evaluated by spectrophotometric or electrophoretic methods. All mite extracts exhibited some form of phosphatase, esterase, aminopeptidase, and glycosidase activity, although their substrate specificities varied considerably. Itch mite extract did not possess detectable serine peptidase activity nor was it able to hydrolyze gelatin or casein, whereas all other mite extracts exhibited these activities. Storage mite extracts possessed enzymes capable of degrading the widest range of substrates, whereas itch mite extract had the most limited proteolytic capacity. Extracts of nine species of allergy-causing astigmatid mites contain wide and diverse repertoires of enzymatic activities. These catalytic activities may be important contributors to the induction and manifestation of inflammatory and immune responses to mites in patients.

  18. Expression and characterisation of a Psoroptes ovis glutathione S-transferase.

    Science.gov (United States)

    Lee, Alison J; Huntley, John; Van den Broek, Adri; Coates, David; Isaac, R Elwyn

    2002-04-19

    The astigmatid mite Psoroptes ovis is the causative agent of sheep scab, a highly contagious parasitic disease of sheep. Infection causes severe allergic dermatitis, resulting in damage to the fleece and hide, loss of condition and occasional mortality. Interest in the P. ovis allergens led us to characterise a glutathione S-transferase (GST) which displays homology to GST allergens isolated from the house dust mite, Dermatophagoides pteronyssinus and the cockroach, Blatella germanica. A cDNA encoding a mu-class GST from P. ovis was expressed in Escherichia coli and the recombinant protein purified for biochemical analysis. SDS-PAGE analysis indicated that the purified product was homogeneous and had an apparent molecular weight of 30 kDa. The recombinant GST (rGST) is active towards the substrate 1-chloro-2,4-dinitrobenzene (CDNB), whereas 1,2-dichloro-4-nitrobenzene (DCNB) is a poor substrate. The recombinant protein was also tested for recognition by IgE and IgG antibodies in serum from P. ovis naïve and P. ovis infested sheep. Neither IgE nor IgG antibodies were detected to the rGST. Prausnitz--Küstner testing with rGST did not provoke a characteristic weal and flare response. Biopsies collected at the PK test sites were stained for eosinophils, neutrophils, mast cells and basophils. Neutrophil, mast cell and basophil counts were not significantly different to the controls. Eosinophil numbers were significantly higher than controls, but were not due to an IgE response.

  19. Lactic acid bacteria inhibit TH2 cytokine production by mononuclear cells from allergic patients.

    Science.gov (United States)

    Pochard, Pierre; Gosset, Philippe; Grangette, Corinne; Andre, Claude; Tonnel, André-Bernard; Pestel, Joël; Mercenier, Annick

    2002-10-01

    Among factors potentially involved in the increased prevalence of allergic diseases, modification of the intestinal bacteria flora or lack of bacterial stimulation during childhood has been proposed. Lactic acid bacteria (LAB) present in fermented foods or belonging to the natural intestinal microflora were shown to exert beneficial effects on human health. Recent reports have indicated their capacity to reduce allergic symptoms. The purpose of this investigation was to determine the effect of LAB on the production of type 2 cytokines, which characterize allergic diseases. PBMCs from patients allergic to house dust mite versus those from healthy donors were stimulated for 48 hours with the related Dermatophagoides pteronyssinus allergen or with a staphylococcal superantigen. The effect of LAB preincubation was assessed by measuring the type 2 cytokine production by means of specific ELISA. The tested gram-positive LAB were shown to inhibit the secretion of T(H)2 cytokines (IL-4 and IL-5). This effect was dose dependent and was observed irrespective of the LAB strain used. No significant inhibition was induced by the control, gram-negative Escherichia coli TG1. Interestingly, LAB reduced the T(H)2 cytokine production from allergic PBMCs specifically restimulated with the related allergen. The inhibition mechanism was shown to be dependent on antigen-presenting cells (ie, monocytes) and on the involvement of IL-12 and IFN-gamma. The tested LAB strains were demonstrated to exhibit an anti-T(H)2 activity, and thus different strains of this family might be useful in the prevention of allergic diseases.

  20. Allergen-stimulated T lymphocytes from allergic patients induce vascular cell adhesion molecule-1 (VCAM-1) expression and IL-6 production by endothelial cells.

    Science.gov (United States)

    Delneste, Y; Jeannin, P; Gosset, P; Lassalle, P; Cardot, E; Tillie-Leblond, I; Joseph, M; Pestel, J; Tonnel, A B

    1995-01-01

    Adhesion of inflammatory cells to endothelium is a critical step for their transvascular migration to inflammatory sites. To evaluate the relationship between T lymphocytes (TL) and vascular endothelium, supernatants from allergen-stimulated TL obtained from patients sensitive to Dermatophagoides pteronyssinus (Dpt) versus healthy subjects were added to endothelial cell (EC) cultures. TL were stimulated by autologous-activated antigen-presenting cells (APC) previously fixed in paraformaldehyde to prevent monokine secretion. Two parameters were measured: the expression of adhesion molecule and the production of IL-6. Related allergen-stimulated TL supernatants from allergic patients induced an increase of VCAM-1 and intercellular adhesion molecule-1 (ICAM-1) expression when supernatants of the control groups (TL exposed to an unrelated allergen or not stimulated or TL obtained from healthy subjects) did not. E-selectin expression was not modulated whatever the supernatant added to EC culture. IL-6 production by EC was significantly enhanced after activation with related allergen-stimulated TL supernatants from allergics compared with control supernatants. Induction of VCAM-1 expression was inhibited by adding neutralizing antibodies against IL-4, whereas IL-6 production and ICAM-1 expression were inhibited by anti-interferon-gamma (IFN-gamma) antibodies. Enhanced production of IL-4 and IFN-gamma was detected in related allergen-stimulated TL supernatants from allergic subjects compared with the different supernatants. These data suggest that allergen-specific TL present in the peripheral blood of allergic patients are of Th1 and Th2 subtypes. Their stimulation in allergic patients may lead to the activation of endothelial cells and thereby participate in leucocyte recruitment towards the inflammatory site. PMID:7542574

  1. Der p 1-pulsed myeloid and plasmacytoid dendritic cells from house dust mite-sensitized allergic patients dysregulate the T cell response.

    Science.gov (United States)

    Charbonnier, Anne-Sophie; Hammad, Hamida; Gosset, Philippe; Stewart, Geoffrey A; Alkan, Sefik; Tonnel, André-Bernard; Pestel, Joël

    2003-01-01

    Although reports suggest that dendritic cells (DC) are involved in the allergic reaction characterized by a T helper cell type 2 (Th2) profile, the role of myeloid (M-DC) and plasmacytoid DC (P-DC), controlling the balance Th1/Th2, remains unknown. Here, we showed that in Dermatophagoides pteronyssinus (Dpt)-sensitized allergic patients and in healthy donors, M-DC displayed a higher capacity to capture Der p 1, a major allergen of Dpt, than did P-DC. However, Der p 1-pulsed M-DC from healthy subjects overexpressed CD80 and secreted interleukin (IL)-10, whereas M-DC from allergic patients did not. In contrast, with Der p 1-pulsed P-DC from both groups, no increase in human leukocyte antigen-DR, CD80, and CD86 and no IL-10 secretion were detected. When cocultured with allogeneic naive CD4(+) T cells from healthy donors, Der p 1-pulsed M-DC from allergic patients favored a Th1 profile [interferon (IFN)-gamma(high)/IL-4(low)] and Der p 1-pulsed P-DC, a Th2 profile (IFN-gamma(low)/IL-4(high)). In healthy donors, no T cell polarization (IFN-gamma(low)/IL-4(low)) was induced by Der p 1-pulsed M-DC or P-DC, but in response to Der p 1-pulsed M-DC, T cells secreted IL-10. The neutralization of IL-10 produced by Der p 1-pulsed M-DC from healthy donors led to an inhibition of IL-10 production by T cells and a polarization toward a type 1. Thus, IL-10 produced by M-DC might be an essential mediator controlling the balance between tolerance and allergic status. In addition, P-DC could contribute to the steady state in healthy donors or to the development of a Th2 response in allergic donors.

  2. Monocyte-derived dendritic cells exposed to Der p 1 allergen enhance the recruitment of Th2 cells: major involvement of the chemokines TARC/CCL17 and MDC/CCL22.

    Science.gov (United States)

    Hammad, Hamida; Smits, Hermelijn H; Ratajczak, Céline; Nithiananthan, Asokananthan; Wierenga, Eddy A; Stewart, Geoffrey A; Jacquet, Alain; Tonnel, Andre-Bernard; Pestel, Joël

    2003-01-01

    Dendritic cells (DC) are potent antigen - presenting cells that can orientate the immune response towards a Th1 or a Th2 type. DC produce chemokines that are involved in the recruitment of either Th1 cells, such as IP10 (CXCL10), Th2 cells such as TARC (CCL17) and MDC (CCL22), or non-polarized T cells such as RANTES (CCL5) and MIP-lalpha (CCL3). We investigated whether monocyte-derived DC (MD-DC) generated from healthy donors or from patients sensitive to Dermatophagoides pteronyssinus (Dpt) and exposed to the cysteine-protease Der p 1(allergen of Dpt), could upregulate the expression of chemokines involved in type 1 or type 2 T cell recruitment. MD-DC were pulsed with either Der p 1 or with LPS as the control and the chemokines produced were evaluated using ELISA and chemotaxis assays. Der p 1-pulsed DC from allergic patients showed increased TARC (CCL17) and MDC (CCL22) production without modifying IP-10 (CXCL10) release. Der p 1-pulsed DC from healthy donors showed only increased IP-10 (CXCL10) secretion. RANTES (CCL5) and MIP-lalpha (CCL3) production were similarly increased when DC were from healthy or allergic donors. The selective Th2 clone recruitment activity of supernatants from Der p 1-pulsed DC of allergic patients was inhibited by anti-TARC (CCL17) and anti-MDC (CCL22) neutralizing Abs. By using anti-IP10 (CXCL10) blocking Abs, supernatants of Der p 1-pulsed DC from healthy donors were shown to be involved in the recruitment of Th1 cells. These results suggest that in allergic patients exposed to house dust mites, DC may favour the exacerbation of the Th2 response via the increase in type 2 chemokine production. Copyright John Libbey Eurotext 2003.

  3. Asthmatic bronchial epithelium activated by the proteolytic allergen Der p 1 increases selective dendritic cell recruitment.

    Science.gov (United States)

    Pichavant, Muriel; Charbonnier, Anne-Sophie; Taront, Solenne; Brichet, Anne; Wallaert, Benoît; Pestel, Joel; Tonnel, André-Bernard; Gosset, Philippe

    2005-04-01

    Airway dendritic cells (DCs) are crucial for allergen-induced sensitization and inflammation in allergic asthma. After allergen challenge, an increased number of DCs is observed in airway epithelium from patients with allergy. Because Der p 1, a cysteine protease allergen from Dermatophagoides pteronyssinus , induces chemokine production by bronchial epithelial cells (BECs), the purpose of this investigation was to evaluate the capacity of BEC exposed to Der p 1 to recruit DCs. Chemotactic activity of BEAS-2B, a bronchial epithelial cell line, and BECs from nonatopic controls and patients with allergic asthma was evaluated on the migration of precursors, immature and mature monocyte-derived DCs (MDDCs), and CD34 + -derived Langerhans cells (LCs). C-C chemokine ligand (CCL)-2, CCL5, and C-X-C chemokine ligand 10 production by BEAS-2B and BEC was increased after Der p 1 exposure, whereas the proenzyme proDer p 1 devoid of enzymatic activity had no effect. Der p 1 stimulation of BEAS-2B and BEC from both groups increased significantly the recruitment of MDDC precursors, depending on CCL2, CCL5, and C-X-C chemokine ligand 10 production. In a reconstituted polarized epithelium, apical application of Der p 1 enhanced MDDC precursor migration into the epithelial layer. Moreover, Der p 1 stimulation of BEC from patients with asthma but not from controls increased the migration of LC precursors, mainly dependent on CCL20 secretion. No migration of immature and mature DCs was observed. These data confirmed that BECs participate in the homeostasis of the DC network present within the bronchial epithelium through the secretion of chemokines. In allergic asthma, upregulation of CCL20 production induced LC recruitment, the role of which remains to be determined.

  4. Control of exposure to mite allergen and allergen-impermeable bed covers for adults with asthma.

    Science.gov (United States)

    Woodcock, Ashley; Forster, Louise; Matthews, Edward; Martin, Jeannett; Letley, Louise; Vickers, Madge; Britton, John; Strachan, David; Howarth, Peter; Altmann, Daniel; Frost, Christopher; Custovic, Adnan

    2003-07-17

    The effectiveness of avoidance of house-dust-mite allergen (Dermatophagoides pteronyssinus 1 [Der p1]) in the management of asthma is uncertain. We conducted a double-blind, randomized, placebo-controlled study of allergen-impermeable bed covers involving 1122 adults with asthma. The primary outcomes were the mean morning peak expiratory flow rate over a four-week period during the run-in phase and at six months and the proportion of patients who discontinued inhaled corticosteroid therapy as part of a phased-reduction program during months 7 through 12. Der p1 was measured in mattress dust in a 10 percent random subsample of homes at entry and at 6 and 12 months. The prevalence of sensitivity to dust-mite allergen was 65.4 percent in the group supplied with allergen-impermeable bed covers (active-intervention group) and 65.1 percent in the control group supplied with non-impermeable bed covers. The concentration of Der p1 in mattress dust was significantly lower in the active-intervention group at 6 months (geometric mean, 0.58 microg per gram vs. 1.71 microg per gram in the control group; P=0.01) but not at 12 months (1.05 microg per gram vs. 1.64 microg per gram; P=0.74). The mean morning peak expiratory flow rate improved significantly in both groups (from 410.7 to 419.1 liters per minute in the active-intervention group, Pimpermeable covers, as a single intervention for the avoidance of exposure to dust-mite allergen, seem clinically ineffective in adults with asthma. Copyright 2003 Massachusetts Medical Society

  5. Characterization of an immunomodulatory Der p 2-FIP-fve fusion protein produced in transformed rice suspension cell culture.

    Science.gov (United States)

    Su, Chin-Fen; Kuo, I-Chun; Chen, Peng-Wen; Huang, Chiung-Hui; Seow, See Voon; Chua, Kaw Yan; Yu, Su-May

    2012-02-01

    Der p 2, a major allergen of Dermatophagoides pteronyssinus mites, is one of the most clinically relevant allergens to allergic patients worldwide. FIP-fve protein (Fve) from the golden needle mushroom (Flammulina velutipes) is an immunomodulatory protein with potential Th1-skewed adjuvant properties. Here, we produced and immunologically evaluated a Der p 2-Fve fusion protein as a potential immunotherapeutic for allergic diseases. Using an inducible expression system in cultured rice suspension cells, the recombinant Der p 2-Fve fusion protein (designated as OsDp2Fve) was expressed in rice cells under the control of an α-amylase gene (αAmy8) promoter and secreted under sucrose starvation. OsDp2Fve was partially purified from the cultured medium. The conformation of Der p 2 in OsDp2Fve remains intact as reflected by its unaltered allergenicity, as assessed by human IgE ELISA and histamine release assays, compared to non-fusion Der p 2 protein. Furthermore, the Fve protein expressed in OsDp2Fve retains its in vitro lymphoproliferative activity but loses its hemagglutination and lymphoagglutination effects compared to the native protein. Notably, in vivo evaluation showed that mice administered with OsDp2Fve possessed an enhanced production of Der p 2-specific IgG antibodies without potentiating the production of Der p 2-specific IgE and Th2 effector cytokines in comparison with mice co-administered with native Fve and Der p 2 proteins. These results suggest that the recombinant Der p 2-Fve fusion protein produced in rice suspension cell cultures has a great potential for allergy immunotherapy.

  6. Innate immune response of alveolar macrophage to house dust mite allergen is mediated through TLR2/-4 co-activation.

    Directory of Open Access Journals (Sweden)

    Chia-Fang Liu

    Full Text Available House dust mite, Dermatophagoides pteronyssinus (Der p, is one of the major allergens responsible for allergic asthma. However, the putative receptors involved in the signalization of Der p to the innate immune cells are still poorly defined as well as the impact of their activation on the outcome of the allergen-induced cell response. We previously reported that the HDM activation of mouse alveolar macrophages (AM involves the TLR4/CD14 cell surface receptor complex. Here using a TLR ligand screening essay, we demonstrate that HDM protein extract engages the TLR2, in addition to the TLR4, in engineered TLR-transfected HEK cells but also in the MH-S mouse alveolar macrophage cell line model. Moreover we found that the concomitant recruitment of the MH-S cell's TLR2 and TLR4 receptors by the HDM extract activates the MyD88-dependent signaling pathway and leads to the secretion of the NF-κB regulated pro-inflammatory factors NO and TNF-α. However unlike with the canonical TLR4 ligand (i.e. the bacterial LPS mobilization of TLR4 by the HDM extract induces a reduced production of the IL-12 pro-inflammatory cytokine and fails to trigger the expression of the T-bet transcription factor. Finally we demonstrated that HDM extract down-regulates LPS induced IL-12 and T-bet expression through a TLR2 dependent mechanism. Therefore, we propose that the simultaneous engagement of the TLR2 and TLR4 receptors by the HDM extract results in a cross regulated original activation pattern of the AM which may contribute to the Th2 polarization of the allergen-induced immune response. The deciphering of these cross-regulation networks is of prime importance to open the way for original therapeutic strategies taking advantage of these receptors and their associated signaling pathways to treat allergic asthma.

  7. HOUSE DUST MITE CONTAMINATION IN HOTELS AND INNS IN BANDAR ABBAS, SOUTH OF IRAN

    Directory of Open Access Journals (Sweden)

    M. Soleimani, J. Rafinejad

    2008-07-01

    Full Text Available House dust mites have been shown to be strongly associated with allergic respiratory diseases such as, bronchial asthma, rhinitis and atopic dermatitis in the world. The climatic conditions of Bandar-Abbas, which is located in a coastal area and has a humid subtropical climate, provide a suitable place to proliferate mites. The aim of this study was to determine the contamination rate and analyze the house dust mite fauna in hotels and inns in Bandar-Abbas that had not been investigated previously. In this study 6 hotels and 6 inns were selected randomly in six areas of Bandar-Abbas. Two dust samples were collected from each place with a vacuum cleaner. One square meter of carpets and mattresses were vacuumed for a period of 1 min. Then the samples were cleared in lactic acid and then mites were mounted in Hoyer's medium for study and identification. A total of 2644 mites were collected and identified. The major mite family was Pyroglyphidae (98%. Dermatophagoides pteronyssinus was the most frequent and most numerous species recorded, occurring in 91% of samples examined and forming 88% of the Pyroglyphidae and 86% of the total mite populations. The family Cheyletidae was less commonly found with Cheyletus malaccensis (2%. Most of the mites were isolated from the carpets (57.5%, and a smaller number from mattresses (42.5%. Mites were present in 96% of the dust samples. Results revealed that all inns and 83% of hotels were contaminated by more than one species of mite and 34% of them had a population of more than 100 mites /g dust. This rate of contamination can be a major risk factor in asthma and other respiratory allergic diseases

  8. Clinical comparison of human and canine atopic dermatitis using human diagnostic criteria (Japanese Dermatological Association, 2009): proposal of provisional diagnostic criteria for canine atopic dermatitis.

    Science.gov (United States)

    Terada, Yuri; Nagata, Masahiko; Murayama, Nobuo; Nanko, Hiroko; Furue, Masutaka

    2011-08-01

    Atopic dermatitis (AD) is a common skin disease encountered in both humans and dogs. Canine AD can be used in the analysis of naturally occurring AD; however, details of clinical comparison have been lacking. The purpose of this study is to compare those clinical features using the human diagnostic criteria (Japanese Dermatological Association, 2009). Fifty-one dogs with canine AD were evaluated by the human criteria. Prior to this study, canine AD was basically diagnosed by the fulfillment of two authentic canine AD criteria and a positive reaction against Dermatophagoides farinae in serum immunoglobulin E levels and/or in intradermal tests. Among the human AD criteria items, behavior corresponding to pruritus was observed in all 51 dogs. Skin lesions corresponding to eczematous dermatitis were seen in 50 dogs, and symmetrical distribution of skin lesions was noted in all 51 dogs. A chronic or chronically relapsing course was observed in 50 dogs. Based on these results, the concordance rate for the criteria was 96% (49/51). Differential diagnoses of AD were also investigated in the same manner. The concordance rate for the criteria was 0% (0/69) in scabies, 2% (1/50) in pyoderma, 0% (0/50) in demodicosis, 0% (0/9) in cutaneous lymphoma, 0% (0/2) in ichthyosis, 25% (2/7) in flea allergy, 48% (24/50) in seborrheic dermatitis and 75% (3/4) in food allergy. Canine AD is thus indicated as a valuable counterpart to human AD in clinical aspects. In addition, the human AD criteria could be applicable, with some modification, as provisional diagnostic criteria for canine AD.

  9. Hyperoxygenation attenuated a murine model of atopic dermatitis through raising skin level of ROS.

    Directory of Open Access Journals (Sweden)

    Hyung-Ran Kim

    Full Text Available Atopic dermatitis (AD is a chronic inflammatory skin disease resulting from excessive stimulation of immune cells. Traditionally, reactive oxygen species (ROS have been implicated in the progression of inflammatory diseases, but several opposing observations suggest the protective role of ROS in inflammatory disease. Recently, we demonstrated ROS prevented imiquimod-induced psoriatic dermatitis through enhancing regulatory T cell function. Thus, we hypothesized AD might also be attenuated in elevated levels of ROS through tissue hyperoxygenation, such as by hyperbaric oxygen therapy (HBOT or applying an oxygen-carrying chemical, perfluorodecalin (PFD. Elevated levels of ROS in the skin have been demonstrated directly by staining with dihydroethidum as well as indirectly by immunohistochemistry (IHC for indoleamine 2,3-dioxygenase (IDO. A murine model of AD was developed by repeated application of a chemical irritant (1% 2,4-dinitrochlorobenzene and house dust mite (Dermatophagoide farinae extract on one ear of BALB/c mice. The results showed treatment with HBOT or PFD significantly attenuated AD, comparably with 0.1% prednicarbate without any signs of side effects, such as telangiectasia. The expressions of interleukin-17A and interferon-γ were also decreased in the AD lesions by treatment with HBOT or PFD. Enhanced expression of IDO and reduced level of hypoxia-inducible factor-1α, in association with increased frequency of FoxP3+ regulatory T cells in the AD lesions, might be involved in the underlying mechanism of oxygen therapy. Taken together, it was suggested that tissue hyperoxygenation, by HBOT or treatment with PFD, might attenuate AD through enhancing skin ROS level.

  10. 变应性鼻炎患者血清总IgE和特异性IgE分析%Survey on distribution of serum total IgE and specific IgE in patients with allergic rhinitis in Shenzhen district

    Institute of Scientific and Technical Information of China (English)

    杨利桃; 黄秋花; 贾小莉

    2014-01-01

    Objective Toexplorethedistributivecharacteristicsofinhaledallergensresponsibleforallergicrhinitisinpopulationof Shenzhendistrictandtoprovidethescientificbasisforitsprevention,etiologicaldiagnosisandtreatment.Methods TheMediwissAllergen ScreeningSystemhadbeenappliedfordetectionoftotalserumIgEandallergen-specificIgEin569patientswithallergicrhinitis.Results The positive rate for Dermatophagoides farinae was the highest(62. 6%),followed by cockroach,dog dander and house dust,and their positive rates were 14. 4%,14. 1% and 10. 0% respectively. There was no significant statistical difference between males and females( P >0. 05),but there was difference between age groups. The positive rate of children group with allergic rhinitis was 87. 8%,and it was much higher than that of adult group( P 0.05),但儿童组吸入性过敏原阳性率为87.8%,成人组吸入性过敏原阳性率为65.4%,两者差异显著( P <0.0001);血清总IgE阳性率为81.9%,吸入性过敏原特异性IgE阳性率为74.2%,总IgE阳性率明显高于特异性IgE阳性率,差异有统计学意义( P <0.0001)。结论户尘螨是诱发深圳特区变应性鼻炎的主要过敏原;儿童AR患者过敏原阳性率高于成人AR患者,可能与其免疫系统发育尚不完善有关;联合检测血清总IgE和过敏原特异性IgE可提高变应性鼻炎诊断的准确性。

  11. Direct analysis of airborne mite allergen (Der f1) in the residential atmosphere by chemifluorescent immunoassay using bioaerosol sampler.

    Science.gov (United States)

    Miyajima, Kumiko; Suzuki, Yurika; Miki, Daisuke; Arai, Moeka; Arakawa, Takahiro; Shimomura, Hiroji; Shiba, Kiyoko; Mitsubayashi, Kohji

    2014-06-01

    Dermatophagoides farinae allergen (Der f1) is one of the most important indoor allergens associated with allergic diseases in humans. Mite allergen Der f1 is usually associated with particles of high molecular weight; thus, Der f1 is generally present in settled dust. However, a small quantity of Der f1 can be aerosolized and become an airborne component. Until now, a reliable method of detecting airborne Der f1 has not been developed. The aim of this study was to develop a fiber-optic chemifluorescent immunoassay for the detection of airborne Der f1. In this method, the Der f1 concentration measured on the basis of the intensity of fluorescence amplified by an enzymatic reaction between the labeled enzyme by a detection antibody and a fluorescent substrate. The measured Der f1 concentration was in the range from 0.49 to 250 ng/ml and a similar range was found by enzyme-linked immunosorbent assay (ELISA). This method was proved to be highly sensitive to Der f1 compared with other airborne allergens. For the implementation of airborne allergen measurement in a residential environment, a bioaerosol sampler was constructed. The airborne allergen generated by a nebulizer was conveyed to a newly sampler we developed for collecting airborne Der f1. The sampler was composed of polymethyl methacrylate (PMMA) cells for gas/liquid phases and some porous membranes which were sandwiched in between the two phases. Der f1 in air was collected by the sampler and measured using the fiber-optic immunoassay system. The concentration of Der f1 in aerosolized standards was in the range from 0.125 to 2.0 mg/m(3) and the collection rate of the device was approximately 0.2%.

  12. Effects of house dust mite sublingual immunotherapy in children with allergic rhinitis and asthma%不同疗程舌下含服粉尘螨滴剂治疗螨过敏变应性鼻炎伴哮喘患儿的疗效观察

    Institute of Scientific and Technical Information of China (English)

    陈实; 曾霞; 王灵; 陈冰; 陈丽丽; 吴少皎; 廖锋; 冯小伟

    2015-01-01

    Objective To evaluate the efficacy of SLIT with Dermatophagoides farinae drops in children with allergic rhinitis and allergic asthma according to its different duration.Methods The efficacy of Dermatophagoides farinae SLIT in 100 children (aged 3-14 yr) with allergic rhinitis and allergic asthma induced by house dust mites was retrospectively analyzed.All children were treated with Dermatophagoides farinae drops for 4 years and followed-up at the interval of 3 months.Total nasal symptom scores (TNSS),total rhinitis symptomatic medication scores (TRMS),daytime asthma symptom scores (DASS),nighttime asthma symptom scores (NASS),total asthma symptomatic medication scores (TAMS),visual analog scale scores (VAS) and lung function were assessed during follow-up visit.SPSS 20.0 software was used for data analysis.Results After 2 years' SLIT,compared with 1-year duration,besides NASS (0.00 [0.00;0.00],0.00[0.00;0.00];Z =1.811;P >0.05),TNSS (2.00[1.00;2.00],0.00[0.00;0.00];Z =7.021,P<0.01),TRMS (2.00[2.00;2.00],0.00[0.00;0.00];Z=8.855;P<0.01),VAS scores (3.00[2.00;4.00],1.00[0.00;1.00];Z =4.494,P<0.01),DASS (1.00[0.00;1.00];0.00[0.00;0.00];Z =4.383,P <0.01) and TAMS (0.00[0.00;1.00],0.00[0.00;0.00];Z =8.944;P < 0.01) all showed significant improvement.After 3-year duration,compared with 2-year duration,VAS scores (1.00 [0.00;1.00],0.00 [0.00;0.00];Z =3.645,P < 0.05) in patients were significantly decreased.Other results showed no significant difference.The comparison of efficacy between 3 and 4-year duration showed no significant difference in global clinical outcomes (all P > 0.05).Conclusions Patients receiving 2 year' SLIT achieve more clinical benefits than those who receiving 1-year duration.The higher efficacy of 3-year duration compared with 2-year duration is supported by a significant improvement in VAS.Besides,the comparison between 3 and 4-year duration shows no significant difference in global clinical outcomes.Therefore,3 years' duration is

  13. Urticaria in Relation to Mite Sensitivity and Immunotherapy with Injectio dermatophagoidei farinae%荨麻疹与螨性过敏的关系及粉尘螨注射液免疫治疗

    Institute of Scientific and Technical Information of China (English)

    邢道荣; 温廷桓; 余扬林; 魏志平; 李益明; 韩天

    2008-01-01

    目的 检测荨麻疹和其他皮疹患者对螨的敏感性,并观察粉尘螨注射液特异性免疫治疗(SIT)荨麻疹螨敏阳性者的临床疗效.方法 1998-2005年对皮肤科门诊的皮疹患者采用粉尘螨(Df)变应原皮肤点刺试验(SPT)检测螨敏情况.将荨麻疹螨敏反应SPT≥++患者分成3组:A组粉尘螨注射液常规特异性免疫治疗,皮下注射1:100000(w/v)03、0.6和1.0ml.各剂量1次/周×3周,1:10000和1:5000(w/v)的剂量、疗程同上,即剂量递增期共9周;1:5000(w/v)1.0ml/(次·周)×6周,即维持剂量期.B组粉尘螨注射液冲击特异性免疫治疗,3个浓度各治疗1 d,各浓度的3个剂量间隔30 min,即剂量递增期共3 d;1:5000(w/v)1.0ml/(次·d)×6d,即维持剂量期.A组和B组各1疗程后改用维持量1:5 000(w/v)1.0 ml每2周1次,维持1年.C组为对照组,用抗组胺药物治疗,依巴斯汀10mg/d+盐酸西替利嗪10mg/d,7d为1个疗程(以后需要时用).观察各组的治疗效果.用ELISA测定20患者粉尘螨注射液特异性免疫治疗前后血清总IgE(tIgE)和粉尘螨特异性IgE(sIgE)水平的变化.结果 2685例皮疹患者中,荨麻疹患者螨敏阳性率为70.3%(1754/2496),高于湿疹患者的63.5%(54/85)和过敏性紫癜患者的60.6%(63/104)(P55.0%)(P<0.05).20例荨麻疹螨敏患者tIgE水平特异性免疫治疗1年后比免疫治疗前下降,粉尘螨slgE水平显著卜升(P<0.01).结论 尘螨变应原皮肤点刺试验对荨麻疹螨敏具有病因诊断价值,粉尘螨注射液特异性免疫治疗对荨麻疹螨敏者有较好的疗效.此外,冲击免疫治疗起效快且优于常规免疫治疗.

  14. Sensibilização a alérgenos inalantes e alimentares em crianças brasileiras atópicas, pela determinação in vitro de IgE total e específica: Projeto Alergia (PROAL Sensitization to inhalant and food allergens in Brazilian atopic children by in vitro total and specific IgE assay: Allergy Project - PROAL

    Directory of Open Access Journals (Sweden)

    Charles K. Naspitz

    2004-06-01

    Full Text Available OBJETIVO: Determinar a freqüência de sensibilização a alérgenos inalantes e alimentares em crianças atendidas em serviços brasileiros de alergia. PACIENTES E MÉTODOS: IgE sérica total e específica (RAST a alérgenos inalantes e alimentares (UniCAP® - Pharmacia foram determinados em 457 crianças acompanhadas em serviços de alergia pediátrica e em um grupo de controles (n = 62. Resultados classe igual ou maior que 1 foram considerados positivos (R+. RESULTADOS: A freqüência de R+ foi significantemente maior entre os atópicos (361/457, 79% quando comparados aos controles (16/62, 25,8%. Não houve diferenças quanto ao sexo. A prevalência de R+ entre os atópicos foi significantemente maior para todos os alérgenos avaliados. Os níveis séricos de IgE total foram significantemente mais elevados entre os atópicos com R+ quando comparados aos com R-. Comparando-se atópicos e controles, a freqüência de R+ para os principais alérgenos inalantes foi como segue: D. pteronyssinus = 66,7 versus 14,5% (p OBJECTIVE: To determine the frequency of sensitization to inhalant and food allergens in children seen at Brazilian allergy services. PATIENTS AND METHODS: Total and specific IgE serum levels to inhalant and food allergens (RAST, UniCAP® - Pharmacia were measured in 457 children accompanied in pediatric allergy services and in 62 control children age matched. RAST equal or higher than class 1 was considered as positive (R+. RESULTS: Frequency of R+ was significantly higher among atopics (361/457, 79% when compared to controls (16/62, 25.8%. There were no differences according to gender. The frequency of R+ to all allergens evaluated were higher among atopics when compared to controls. Significantly higher total IgE serum levels were observed among the atopics with R+ in comparison to those with R-. The frequency of R+ to main inhalant allergens were: D. pteronyssinus = 66.7% x 14.5% (p < 0.05, D. farinae = 64.5% x 17.8% (p < 0.05, B

  15. Phadiatop® no diagnóstico de alergia respiratória em crianças: Projeto Alergia (PROAL Phadiatop® in the diagnosis of respiratory allergy in children: Allergy Project - PROAL

    Directory of Open Access Journals (Sweden)

    Charles K. Naspitz

    2004-06-01

    Full Text Available OBJETIVO: Avaliar a positividade do Phadiatop® em crianças acompanhadas em serviços brasileiros de alergologia e compará-la aos resultados de IgE sérica específica a alérgenos inalantes e alimentares. CASUÍSTICA E MÉTODO: Em 457 crianças acompanhadas em serviços de alergia pediátrica e um grupo de crianças controle não-alérgicas (n = 62, distribuídas em cinco faixas etárias, foram determinados em amostra de soro: Phadiatop® e IgE específica (RAST a alérgenos inalantes e alimentares (UniCAP - Pharmacia®. RESULTADOS: O Phadiatop® foi positivo em 305 crianças atópicas (67,6% e em 25,8% das controles (p OBJECTIVES: To evaluate the positivity of Phadiatop® in children from several Brazilian pediatric allergology centers and to compare its results with the presence of serum specific IgE to inhalant and food allergens. PATIENTS AND METHOD: Phadiatop® and serum specific IgE levels (RAST to inhalant and food allergens (UniCAP - Pharmacia® were measured in 457 children from several pediatric allergy centers and in a non-allergic control group (n = 62, distributed across five age groups. RESULTS: Phadiatop was positive in 305 atopic children (67.6% and in 25.8% of controls (p < 0.001. Among atopic children the distribution of positive test varied according to age: 7.9% (24/305 among under 2 year-olds, 15.4% (47/305 in 2 to 3 year-olds, 22% (67/305 in 3 to 4 year-olds, 19.3% (59/305 in 4 to 5 year-olds and 35.4% (108/305 in 5 to 12 year-olds. No concordance between food allergens and Phadiatop® was observed. Analysis of the relationship between positive inhaled allergen RASTs and positive Phadiatop® showed best indices with house dust mites (D. pteronyssinus, D. farinae and Blomia tropicalis. CONCLUSIONS: Phadiatop® is a useful tool for diagnosing domestic mite allergy.

  16. Acaroid mite allergens from the filters of air-conditioning system in China.

    Science.gov (United States)

    Li, Chao-Pin; Guo, Wei; Zhan, Xiao-Dong; Zhao, Bei-Bei; Diao, Ji-Dong; Li, Na; He, Lian-Ping

    2014-01-01

    Accumulation of acaroid mites in the filters of air-conditioners is harmful to human health. It is important to clarify the allergen components of mites from the filters of local air-conditioning system. The present study was to detect the allergen types in the filters of air-conditioners and assesse their allergenicity by asthmatic models. Sixty aliquots of dust samples were collected from air conditioning filters in civil houses in Wuhu area. Total protein was extracted from the dust samples using PBS and quantified by Bradford method. Allergens I and II were also detected by Western blot using primary antibody (anti-Der f1/2, Der p1/Der f2/Der p2, respectively). Ten aliquots of the positive samples were randomly selected for homogenization and sensitized the mice for developing asthmatic animal models. Total serum IgE level and IFN-γ, IL-4 and IL-5 in the bronchoalveolar lavage fluid (BALF). The allergenicity of the extraction was assessed using pathological sections developed from the mouse pulmonary tissues. The concentration of extract from the 60 samples was ranged from 4.37 μg/ml to 30.76 μg/ml. After analyzing with Western blot, 31 of 60 samples were positive for 4 allergens of acaroid mites, and yet 16 were negative. The levels of total IgE from serum IL-4 and IL-5 from the BALF in the experimental group were apparently higher than that of negative control and PBS group (P 0.05). However,the IFN-γ level in BALF was lower compared with the negative control and PBS group (P 0.05). The pathological changes were evidently emerged in pulmonary tissues, which were similar to those of OVA group, compared with the PBS ground and negative controls. The air-conditioner filters in human dwellings of Wuhu area potentially contain the major group allergen 1 and 2 from D. farinae and D. pteronyssinus, which may be associated with seasonal prevalence of allergic disorders in this area.

  17. DNA vaccine encoding Der p 2 allergen generates immunologic protection in recombinant Der p 2 allergen-induced allergic airway inflammation mice model

    Institute of Scientific and Technical Information of China (English)

    LI Guo-ping; LIU Zhi-gang; QIU Jing; RAN Pi-xin; ZHONG Nan-shan

    2005-01-01

    Background DNA immunization is a promising novel type of immunotherapy against allergy. An estimated 79.2% patients with asthma, wheezing and/or rhinitis suffer from Dermatophagoides pteronyssinus group 2 (Der p 2) allegen. The aim of the present study was to determine whether DNA vaccine encoding Der p 2 could generate immunologic protection in recombinant Der p 2 (rDer p 2) allergen-induced allergic airway inflammation mice model and to understand the role of DNA vaccination in specific-allergen immunotherapy for asthma. Methods After DNA vaccination, BALB/c mice were sensitized by intraperitoneal injection (i.p) and challenged by intranasal instillation of rDer p 2. The lung tissues were assessed using hematoxylin and eosin. Mucus-producing goblet cells were identifed using periodic acid-Schiff(PAS)/alcian blue. The total cell number and composition of bronchoalveolar lavage samples were determined. The levels of the cytokines IL-4 and IFN-γ, as well as IgE and IgG2a in the serum were determined by enzyme-linked immunosorbent assay. Allergen-specific IL-4 and IFN-γ production by spleen cells were also measured by enzyme-linked immunosorbent assay. Expression of signal transducer and activator of transcription 6 (STAT6) in splenocytes were determined by Western blot. Results DNA vaccine encoding Der p 2 allergen inhibited extensive infiltration of inflammatory cells and production of mucin induced by allergen. The influx of eosinophils into the lung interstitium was significantly reduced after administration of DNA vaccine. Significant reductions of IL-4 and increase in levels of IFN-γ in bronchoalveolar lavage fluid were observed. The allergen-specific IgE was markedly decreased in mice receiving DNA vaccination. Allergen could induce higher IFN-γ, weaker IL-4 in cultured spleen cells from mice receiving DNA vaccine. DNA vaccination inhibited STAT6 expression of spleen cells induced by allergen. Conclusion These results indicated that DNA vaccine encoding

  18. HOUSE DUST MITE ALLERGEN (Derp1 AND Blot5) LEVELS IN ASTHMATICS' HOME IN HONGKONG

    Institute of Scientific and Technical Information of China (English)

    Bao-qing Sun; Adrian Wu; Albert Chan; Stanley Chik; Dorothy Wong; Nan-shan Zhong

    2004-01-01

    Objective To measure Derpl and Blot5 allergen levels in asthmatics' homes in Hongkong.Methods Seventy houses were enrolled for a mite indoor environment study. Dust samples were obtained from two sites of each patients' house: bed and floor. Derpl and Blot5 levels were quantified by a two-site monoclonal antibody-based ELISA technique.Results The levels of Derpl allergens found in bed (geometric mean (GM) 3.43 μg/g of dust; 95%CI, 1.89-4.96 μg/g)and on the floor (GM 1.12 μg/g of dust; 95%CI, 0.71-1.53 μg/g) indicated significant differences (P=0.005). However, the levels of Blot5 allergens found in bed (GM 19.00 μg/g of dust; 95%CI, 0.89-38.90 μg/g) and on the floor (GM 6.14 μg/g of dust; 95%CI, 0.40-11.90 μg/g) showed no statistically significant difference. In addition, in regards to the exposure index for Derpl and Blot5 allergens found in bed and on the floor, 17.6% in bed and 8.6% on the floor had levels of Blot5 ≥ 10 μg/g of dust, higher than those obtained for Derp1 (7.2% and 0% in bed and on the floor respectively, P< 0.05); higher percentages in bed and on the floor (25.0% and 35.7%) were observed for levels of Blot5 =0 μg/g of dust as compared with Derpl in bed and on the floor (4.3% and 14.5% respectively, P< 0.05).Conclusions Derpl and Blot5 are the major allergens found in this regional study, Blot5 is a more potent allergen in Hongkong, probably reflecting the high level of exposure to Blomia tropicalis (Bt). Bt and Dermatophagoides pteronyssinus (Dp) allergens should be included for precise diagnosis and effective immuno-therapeutic treatment of mite allergy in Hongkong.

  19. Responses of IgE, IgG1, and IgG4 to concanavalin A-binding Blomia tropicalis antigens in allergic patients

    Directory of Open Access Journals (Sweden)

    K.C. Almeida

    2006-11-01

    Full Text Available Blomia tropicalis (Bt and Dermatophagoides pteronyssinus (Dp are the prevalent house dust mites in tropical countries and are associated with allergic diseases. Glycosylated antigens are highly immunogenic and involved in different pathologies. We evaluated the presence of IgE, IgG1, and IgG4 to concanavalin A-binding antigens (Bt-Con-A isolated from Bt-total extract in sera of allergic and non-allergic subjects. Bt-total and Bt-Con-A extracts were evaluated by SDS-PAGE and ELISA for reacting with IgE, IgG1, and IgG4 in sera of 121 patients with allergic rhinitis and 36 non-allergic individuals. All subjects were skin prick tested with Bt-total extract and inhibition tests were performed for IgE, IgG1, and IgG4 using both extracts (Bt-total and Bt-Con-A. Skin prick test showed that 58% of the patients were sensitized to Bt (Bt+, with 52% reactive to both mites (Bt and Dp and 6% to Bt only. A broad spectrum of proteins (14-152 kDa was visualized in Bt-total and components >27 kDa for the Bt-Con-A extract. ELISA showed a similar profile of IgE, IgG1 and IgG4 levels in response to Bt-total and Bt-Con-A extracts in different groups, although Bt+ patients showed a lower IgG4 reactivity to Bt-Con-A extract. Specific IgG1 levels were higher in Bt+ patients than in control subjects, and IgG4 levels showed no significant difference among groups. ELISA inhibition showed a partial IgE and total IgG1 and IgG4 cross-reactivity with Dp extract for Bt-total and Bt-Con-A extracts. We conclude that Con-A-binding components isolated from Bt constitute major allergens and are involved in both allergen sensitization (IgE response and homeostasis maintenance (IgG1 and IgG4 responses.

  20. The presence of serum anti-Ascaris lumbricoides IgE antibodies and of Trichuris trichiura infection are risk factors for wheezing and/or atopy in preschool-aged Brazilian children

    Directory of Open Access Journals (Sweden)

    Alcântara-Neves Neuza M

    2010-08-01

    Full Text Available Abstract Background The elucidation of factors that trigger the development of transient wheezing in early childhood may be an important step toward understanding the pathogenesis of asthma and other allergic diseases later in life. Transient wheezing has been mainly attributed to viral infections, although sensitisation to aeroallergens and food allergens may occur at an early age. In developing countries, intestinal helminthic infections have also been associated with allergy or atopy-related disorders. Objective The aim of this study was to explore the association of Trichuris trichiura and Ascaris lumbricoides infections with wheezing and atopy in early childhood. Study design A cross-sectional study using a Portuguese-language ISAAC phase I questionnaire, adapted for preschool-aged children, nested in a cohort study of childhood diarrhoea, was conducted on 682 children. Two faecal samples per child were examined for the presence of intestinal helminthic infection. IgE antibodies against three allergenic preparations (Dermatophagoides pteronyssinus, Blomia tropicalis and common child food, as well as against A. lumbricoides antigens, were measured in a sub-sample of these children, whose parents allowed the procedure. Atopy was defined by the presence of levels of serum IgE antibodies ≥0.35 kU/L against at least one of the three tested allergenic preparations. Results Active T. trichiura infection but not A. lumbricoides infection was positively associated with wheezing in the total studied children population [adjusted OR = 2.60; CI = 1.54;4.38] and in the atopic children sub-population [adjusted OR = 3.07; CI = 1.00;9.43]. The association with atopy was also positive and statistically significant only in the brute analysis [OR = 2.13; CI = 1.03;4.40]. Anti-A. lumbricoides IgE antibodies, but not current A. lumbricoides infection, were positively associated with wheezing in atopic children [adjusted OR = 2.01; CI = 1.00;4.50] and in non

  1. Adverse reactions and tolerability of high-dose sublingual allergen immunotherapy

    Directory of Open Access Journals (Sweden)

    Moral A

    2016-06-01

    diagnosed (P=0.3800, treatment composition (P=0.6946, polysensitization (P=0.1730, or clinical diagnosis (P=0.3354. However, it was found that treatment duration had a statistically significant influence (3 months, >3 months: P=0.0442 and the presence of asthma was close to statistical significance (P=0.0847.Conclusion: In our study, treatment duration is significantly associated with the occurrence of adverse reactions after the administration of high doses of sublingual allergen immunotherapy. Keywords: sublingual immunotherapy, tolerability, allergic rhinitis, Olea europaea, grass, Dermatophagoides pteronyssinus

  2. PREVALENCIA DE SENSIBILIZACIÓN EN PACIENTES PEDIÁTRICOS CON SOSPECHA O DIAGNÓSTICO DE ENFERMEDAD ALÉRGICA. ESTUDIO PRESPPENAL(1

    Directory of Open Access Journals (Sweden)

    María Claudia Ortega López

    2014-10-01

    Full Text Available Introducción. Las enfermedades alérgicas vienen en aumento en los últimos años tanto en prevalencia como en morbilidad y mortalidad. Se conoce que la sensibilización a uno o varios alérgenos es un factor de riesgo importante para enfermedad alérgica; sin embargo, cuantificar cómo el hecho de estar sensibilizado determina los síntomas clínicos para determinada enfermedad y rango de edad aún es motivo de controversia. Metodología: Presentamos los datos de los resultados de las pruebas positivas de alergia o pruebas de puntura realizadas a 671 de un total de 798 pacientes pediátricos de 1 a 18 años de edad con sospecha o diagnóstico clínico de enfermedad alérgica, con el fin de obtener la prevalencia de la sensibilización para aeroalérgenos y alérgenos alimentarios en tres hospitales de la ciudad de Bogotá; ciudad tropical ubicada a 2.600 metros de altura sobre el nivel del mar, con temperatura de 15°C y humedad relativa en promedio anual de 75%. Resultados: La edad promedio fue de 9.9 años, 369 (55% fueron de sexo masculino. De los aeroalérgenos, el más prevalente fue el Dermatophagoides pteronyssinus, en 493 pacientes (73.5% (IC 95% 70.1-76.8. De los alérgenos alimentarios se encontró resultado positivo en mayor proporción a la yema de huevo en el 4.9%, (IC 95% 3.2-6.3. De las patologías reportadas, la más prevalente fue la rinitis alérgica en 319 (47.5% de los pacientes. Conclusiones: En la población estudiada con patología alérgica definida por diagnóstico clínico se encontró una mayor sensibilización a aeroalérgenos por ácaros del polvo domiciliario. La patología más prevalente asociada a sensibilización con aeroalérgenos fue la rinitis alérgica seguida de asma y dermatitis atópica; en nuestro medio, la sensibilización a pólenes es relativamente baja y por alérgenos alimentarios es muy baja.

  3. Frequent use of paracetamol and risk of allergic disease among women in an Ethiopian population.

    Directory of Open Access Journals (Sweden)

    Alemayehu Amberbir

    Full Text Available INTRODUCTION: The hypothesis that paracetamol might increase the risk of asthma and other allergic diseases have gained support from a range of independent studies. However, in studies based in developed countries, the possibility that paracetamol and asthma are associated through aspirin avoidance is difficult to exclude. OBJECTIVES: To explore this hypothesis among women in a developing country, where we have previously reported aspirin avoidance to be rare. METHODS: In 2005/6 a population based cohort of 1065 pregnant women was established in Butajira, Ethiopia and baseline demographic data collected. At 3 years post birth, an interview-based questionnaire administered to 945 (94% of these women collected data on asthma, eczema, and hay fever in the past 12 month, frequency of paracetamol use and potential confounders. Allergen skin tests to Dermatophagoides pteronyssinus and cockroach were also performed. The independent effects of paracetamol use on allergic outcomes were determined using multiple logistic regression analysis. FINDINGS: The prevalence of asthma, eczema and hay fever was 1.7%, 0.9% and 3.8% respectively; of any one of these conditions 5.5%, and of allergen sensitization 7.8%. Paracetamol use in the past month was reported by 29%, and associations of borderline significance were seen for eczema (adjusted OR (95% CI = 8.51 (1.68 to 43.19 for 1-3 tablets and 2.19 (0.36 to 13.38 for ≥4 tablets, compared to no tablets in the past month; overall p = 0.055 and for 'any allergic condition' (adjusted OR (95% CI = 2.73 (1.22 to 6.11 for 1-3 tablets and 1.35 (0.67 to 2.70 for ≥4 tablets compared to 0 in the past month; overall p = 0.071. CONCLUSIONS: This study provides further cross-sectional evidence that paracetamol use increases the risk of allergic disease.

  4. Indoor mite allergens in patients with respiratory allergy living in Porto, Portugal.

    Science.gov (United States)

    Plácido, J L; Cuesta, C; Delgado, L; da Silva, J P; Miranda, M; Ventas, P; Vaz, M

    1996-09-01

    We investigated the levels of mite allergens (Der p 1, Der f 1, Der 2, and Lep d 1) in dust samples from the homes of 59 patients with asthma, 36 sensitized to house-dust mites (HDM) and 23 to grass pollen (controls), living in Porto, northern Portugal. The relationship between exposure and sensitization to HDM and the influence of housing conditions on mite-allergen levels were also evaluated. Der p 1 (median 9.2 micrograms/g) and Der 2 (4.6 micrograms/g) were the main allergens, while Der f 1 and Lep d 1 levels were always 2 micrograms/g and their homes contained significantly higher levels of Der p 1 (median 12.5 vs 6.4 micrograms/g; P = 0.008) and Der 2 (6.2 vs 3.0 micrograms/g; P = 0.004) when compared to the control group. A significant correlation was observed between the exposure to Der p 1 and the wheal area at skin testing with the Dermatophagoides pteronyssinus (Dp) extract (P = 0.01) as well as with serum specific IgE levels to Dp (P = 0.03). Patients with higher levels of serum specific IgE (> or = 17.5 HRU/ml) were also more frequently exposed to Der p 1 levels > or = 10 micrograms/g (P = 0.002). Old homes, presence of carpets, and signs of dampness were conditions associated with significantly higher levels of mite allergens. In conclusion, we found high levels of Der p 1 and Der 2 particularly in the homes of HDM-sensitized patients and we confirm the relationship between exposure and sensitization to HDM, assessed by both in vivo and in vitro methods. In additional to a favorable outdoor climate, we found in our region housing conditions propitious to mite growth, suggesting that specific geographic characteristics must also be taken into account for the correct planning of mite-avoidance measures.

  5. Report of Common Aeroallergens among Allergic Patients in Northeastern Iran

    Directory of Open Access Journals (Sweden)

    Yaghoub Mahboubi Oskouei

    2017-03-01

    Full Text Available Introduction:The prevalence of atopic diseases has increased in recent decades dramatically. The most common aeroallergens in Northeastern Iran have not been fully defined. Define the most common aeroallergens in allergic patients based on the skin prick test (SPT was aimed in this investigation.Materials and Methods:This cross-sectional study enrolled 1,006 allergic patients (aged 1–86 years from October 2010 to February 2014 referred to the Allergy clinics of Mashhad University of Medical Science. After completing a checklists including demographic information, the SPT was performed according to the patients’ history of aeroallergen sensitivity.Results:Patients with symptoms of asthma allergic rhinitis, atopic dermatitis, and urticaria were enrolled . 97% of patients had a positive skin test to at least one aeroallergen. The most prevalent allergens were Russian thistle (Salsola kali (50.2%, ash (Fraxinus excelsior (36.7%, grass mix (29.1%, tree mix (21.6%, and pigweed mix (19.5%. Common allergens in patients with different symptoms of allergic disorders were as follows: asthma (Russian thistle, grass mix, ash, tree mix, and Dermatophagoides pteronyssinus; allergic rhinitis (Russian thistle,ash, grass mix, tree mix, and pigweed mix; urticaria (Russian thistle, ash, grass mix, pigweed mix, and tree mix and atopic dermatitis (Russian thistle, grass mix, ash, tree mix, and pigweed mix. In the spring, the most prevalent allergens were Russian thistle, ash, grass mix, tree mix, and pigweed mix. In the summer, Russian thistle, ash, grass mix, tree mix, and pigweed mix accounted for the most prevalent allergens. During the autumn, Russian thistle, ash, grass mix, pigweed mix and lamb’s quarter were the most common aeroallergens, while in the winter, Russian thistle, ash, grass mix, pigweed mix, and tree mix were shown to be the most common aeroallergens.Conclusion:Determination of the most common aeroallergens in this area is unavoidable in the

  6. Dissociation between skin test reactivity and anti-aeroallergen IgE: Determinants among urban Brazilian children

    Science.gov (United States)

    Veiga, Rafael V.; Ponte, João C. M.; da Cunha, Sérgio S.; Simões, Silvia M.; Cruz, Álvaro A.; Yazdanbakhsh, Maria; Matos, Sheila M.; Silva, Thiago Magalhães; Figueiredo, Camila A.; Rodrigues, Laura C.; Fiaccone, Rosemeire L.; Cooper, Philip J.; Barreto, Maurício L.

    2017-01-01

    Background The dissociation between specific IgE and skin prick test reactivity to aeroallergens, a common finding in populations living in low and middle-income countries, has important implications for the diagnosis and treatment of allergic diseases. Few studies have investigated the determinants of this dissociation. In the present study, we explored potential factors explaining this dissociation in children living in an urban area of Northeast Brazil, focusing in particular on factors associated with poor hygiene. Methods Of 1445 children from low income communities, investigated for risk factors of allergies, we studied 481 with specific IgE antibodies to any of Blomia tropicalis, Dermatophagoides pteronyssinus, Periplaneta americana and Blatella germanica allergens. Data on demographic, environmental and social exposures were collected by questionnaire; serum IgG and stool examinations were done to detect current or past infections with viral, bacterial, protozoan and intestinal helminth pathogens. We measured atopy by skin prick testing (SPT) and specific IgE (sIgE) to aerollergens in serum (by ImmunoCAP). SIgE reactivity to B. tropicalis extract depleted of carbohydrates was measured by an in-house ELISA. Total IgE was measured by in house capture ELISA. SNPs were typed using Illumina Omni 2.5. Results Negative skin prick tests in the presence of specific IgE antibodies were frequent. Factors independently associated with a reduced frequency of positive skin prick tests were large number of siblings, the presence of IgG to herpes simplex virus, Ascaris lumbricoides and Trichuris trichiura infections, living in neighborhoods with infrequent garbage collection, presence of rodents and cats in the household and sIgE reactivity to glycosylated B. tropicalis allergens. Also, SNP on IGHE (rs61737468) was negatively associated with SPT reactivity. Conclusions A variety of factors were found to be associated with decreased frequency of SPT such as unhygienic

  7. [Oral anaphylaxis by ingestion of mite contaminated food in Panama City, 2011-2014].

    Science.gov (United States)

    Barrera, Olga M; Murgas, Ingrid L; Bermúdez, Sergio; Miranda, Roberto J

    2015-01-01

    Antecedentes: la anafilaxia oral por ácaros, o síndrome de las panquecas, ocurre cuando individuos atópicos ingieren alimentos elaborados con harina de trigo contaminada con ácaros, lo que induce reacciones alérgicas moderadas o severas, incluso fatales. Los reportes provienen de ambientes tropicales y subtropicales, donde las condiciones para la proliferación de ácaros domésticos son favorables. Se ha encontrado una asociación importante con la hipersensibilidad a ácido acetilsalicílico (AAS)-antiinflamatorios no esteroides (AINES) y el ejercicio físico. Objetivo: documentar el estudio clínico y descriptivo de casos de anafilaxia oral por ácaros atendidos en Ciudad de Panamá durante los años 2011 a 2014. Material y método: estudio clínico descriptivo y retrospectivo, que incluyó pruebas cutáneas de punción para cinco especies de ácaros domésticos. La identificación taxonómica de los ácaros se realizó bajo microscopio y se calculó la densidad de ácaros por gramo de alimento en tres casos. Resultados: se estudiaron 10 casos compatibles con anafilaxia oral por ácaros, la panqueca fue el alimento implicado con más frecuencia. Los primeros síntomas aparecieron entre 25 y 60 minutos después de la ingestión del alimento. Todos los pacientes fueron adultos jóvenes atópicos, cuatro con hipersensibilidad a AAS-AINES. Los pacientes resultaron positivos a las pruebas cutáneas de punción para los ácaros: Blomia tropicalis, Dermatophagoides pteronyssinus y Suidasia pontifica. Tres muestras de harina de trigo estudiadas motraron altas densidades de ácaros. Conclusiones: la panqueca fue el alimento implicado con más frecuencia en los casos de anafilaxia oral por ácaros. Suidasia pontifica y Blomia tropicalis parecen ser los ácaros más importantes relacionados con este síndrome en nuestro país.

  8. Analysis of basophil activation by flow cytometry in pediatric house dust mite allergy.

    Science.gov (United States)

    González-Muñoz, Miguel; Villota, Julian; Moneo, Ignacio

    2008-06-01

    Detection of allergen-induced basophil activation by flow cytometry has been shown to be a useful tool for allergy diagnosis. The aim of this study was to assess the potential of this technique for the diagnosis of pediatric house dust mite allergy. Quantification of total and specific IgE and basophil activation test were performed to evaluate mite allergic (n = 24), atopic (n = 23), and non-allergic children (n = 9). Allergen-induced basophil activation was detected as a CD63-upregulation. Receiver operating characteristics (ROC) curve analysis was performed to calculate the optimal cut-off value of activated basophils discriminating mite allergic and non-allergic children. ROC curve analysis yielded a threshold value of 18% activated basophils when mite-sensitized and atopic children were studied [area under the curve (AUC) = 0.99, 95% confidence interval (CI) = 0.97-1.01, p Analysis of the data obtained with 1.6 microg/ml mite extract defined a cut-off value of 8% activated basophils (AUC = 0.96, 95% CI = 0.91-1.01; p basophils (AUC = 1.0) with 16 microg/ml allergen extract and a sensitivity and specificity of 100%. The same threshold and specificity values were obtained with 1.6 microg/ml extract (AUC = 97%, 95% CI = 0.92-1.02; p basophil activation tests and high specific IgE (>43 kU/l) values for Dermatophagoides pteronyssinus allergen. They also showed positive prick (wheal diameter >1.0 cm) and basophil activation (>87%) tests and high specific IgE (>100 kU/l) with shrimp allergen. Shrimp sensitization was demonstrated by high levels of Pen a 1-specific IgE (>100 kU/l). Cross-reactivity between mite and shrimp was confirmed by fluorescence enzyme immunoassay (FEIA-CAP) inhibition study in these two cases. This study demonstrated that the analysis of allergen-induced CD63 upregulation by flow cytometry is a reliable tool for diagnosis of mite allergy in pediatric patients, with sensitivity similar to routine diagnostic tests and a higher specificity

  9. House dust mite allergen Der p 1 elevates the release of inflammatory cytokines and expression of adhesion molecules in co-culture of human eosinophils and bronchial epithelial cells.

    Science.gov (United States)

    Wong, Chun K; Li, Mandy L Y; Wang, Cheng B; Ip, Wai K; Tian, Ya P; Lam, Christopher W K

    2006-08-01

    House dust mite (HDM) is a common allergen of allergic asthma. Eosinophils are principal effector cells of allergic inflammation and their adhesion onto human bronchial epithelial cells is mediated by a CD18-intracellular adhesion molecule-1 (ICAM-1)-dependent interaction. We studied the effects of HDM Dermatophagoides pteronyssinus (Der p) 1 on the activation of eosinophils and bronchial epithelial BEAS-2B cells. Cytokines and adhesion molecules were measured using flow cytometry. Transcription factor nuclear factor-kappaB (NF-kappaB) and activator protein-1 (AP-1) and signaling molecule p38 mitogen-activated protein kinase (MAPK) were analyzed using electromobility shift assay and western blot, respectively. Der p 1 protein was found to potently induce the release of IL-1beta, IL-6, IL-10, tumor necrosis factor (TNF)-alpha and granulocyte macrophage colony-stimulating factor from eosinophils. Such induction was further up-regulated for IL-6 and IL-10, and down-regulated for TNF-alpha and IL-1beta in eosinophil-BEAS-2B cells co-culture. Surface expression of CD18 and ICAM-1 on eosinophils was greatly increased by Der p 1; such inductive effect on ICAM-1 was also found to be more prominent on BEAS-2B cells from the co-culture than BEAS-2B cells alone. Der p 1 was found to activate NF-kappaB and AP-1 activity in eosinophils alone and in co-culture and BEAS-2B cells in co-culture. Moreover, Der p 1 could activate p38 MAPK in BEAS-2B cells and eosinophils alone and in co-culture. Selective inhibition of NF-kappaB, AP-1 and p38 MAPK resulted in differential suppression of the Der p 1-induced cytokine release and adhesion molecule expression. As an allergen, HDM could therefore induce the release of inflammatory cytokines and expression of adhesion molecules from the interaction of human eosinophils and bronchial epithelial cells.

  10. Comparison of the effects of nebulized terbutaline with or without intravenous betamethasone on exhaled nitric oxide in children with acute asthma attack.

    Science.gov (United States)

    Lee, Ming Yung; Tsai, Yi Giien; Yang, Kuender D; Hung, Chih Hsing

    2006-02-01

    Exhaled nitric oxide (eNO), a non-invasive marker that reflects the degree of airway inflammation, may be useful for assessing the response to anti-inflammatory treatment of asthma. The purpose of this randomized prospective study was to compare the effect of a nebulized terbutaline plus a single intravenous dose of betamethasone at baseline followed by a second of terbutaline at 6 h with the effect of the same protocol of nebulized terbutaline alone on airway inflammation of acute asthmatic children as demonstrated by eNO levels. Children visiting the emergency department due to acute asthma attack were recruited. All enrolled patients had fluorescent assay-proven hypersensitivity to Dermatophagoides pteronyssinus. Patients were randomized to receive either nebulized terbutaline plus intravenous betamethasone (experimental group, n = 11) or nebulized terbutaline alone (control group, n = 11) at baseline followed by a second dose of nebulized terbutaline alone 6 h later. Exhaled NO concentrations were significantly reduced in the experimental group at 7 h (40.25 +/- 12.43 vs 28.88 +/- 18.02 ppb; p = 0.005) and 12 h (40.25 +/- 12.43 vs 30.11 +/- 18.16 ppb; p = 0.007) after treatment. The eNO level in the experimental group was also reduced at 7 h (28.88 +/- 18.02 vs 38.12 +/- 16.50 ppb; p = 0.034) and 12 h (30.11 +/- 18.16 vs 39.36 +/- 17.63 ppb; p = 0.035) compared to the control group. The change of eNO concentration was correlated to the change of peak expiratory flow rate (PEFR) [r = -0.678; p = 0.022] and pulmonary index scores (r = 0.606; p = 0.048) at 7 h after treatment in the betamethasone group. Nebulized terbutaline given at baseline and 6 h later was significantly more effective in improving PEFR and asthmatic symptoms (pulmonary index scores) for at least 12 h when the initial dose was administered in combination with intravenous betamethasone.

  11. 南阳市慢性过敏性鼻炎患者过敏原检测结果分析%Analysis of Allergen Detection Results in Patients With Chronic Allergic Rhinitis in Nanyang City

    Institute of Scientific and Technical Information of China (English)

    宋彬

    2015-01-01

    Objective To understand the types and distribution of allergens in patients with chronic allergic rhinitis in nanyang, and to provide reliable evidence for the prevention and treatment of this disease.Methods ELISA method was used for the determination of serum total IgE and specific IgE in patients with chronic allergic rhinitis, and the comparative analysis was carried out.Results Patients with chronic allergic rhinitis in total IgE positive rate of 100%, the 2 cases of specific allergen test negative, specific allergen detection results for dermatophagoides pteronyssinus 113 cases of the positive (37.7%), house dust was positive in 90 cases (30.0%), trees (oak, elm, sycamore, willow, cottonwood yang) was positive in 54 cases (18.0%). Conclusion Nanyang city cause chronic allergic rhinitis allergic principle mainly in household dust mites, house dust, trees (oak, elm, sycamore, willow, cottonwood yang).%目的 了解南阳市慢性过敏性鼻炎患者过敏原的种类及分布,为本病预防和治疗提供可靠依据.方法 采用ELISA法对慢性过敏性鼻炎患者的血清总IgE和特异性IgE进行半定量测定,并进行对比分析.结果 慢性过敏性鼻炎患者中总IgE阳性率100%,其中2例特异性过敏原检测阴性,特异性过敏原检测结果 为:户尘螨阳性113例(37.7%),屋尘阳性90例(30.0%),树木(栎树、榆树、梧桐、柳树、三角叶杨)阳性54例(18.0%).结论 南阳市引起慢性过敏性鼻炎的过敏原主要是户尘螨,屋尘,树木(栎树、榆树、梧桐、柳树、三角叶杨).

  12. Immune response to allergens in sheep sensitized to house dust mite

    Directory of Open Access Journals (Sweden)

    Velden Joanne

    2008-10-01

    Full Text Available Abstract Background House dust mite (HDM allergens are a major cause of allergic asthma. Most studies using animal models of allergic asthma have used rodents sensitized with the 'un-natural' allergen ovalbumin. It has only recently been recognized that the use of animal models based on HDM provide a more relevant insight into the allergen-induced mechanisms that underpin human allergic disease. We have previously described a sheep model of human allergic asthma that uses Dermatophagoides pteronyssinus HDM. The present study extends our understanding of the immune effects of HDM and the allergens Der p 1 and Der p 2 in the sheep model of asthma. Methods Peripheral blood sera from non-sensitized (control sheep and sheep sensitized to HDM was collected to determine immunoglobulin (Ig reactivities to HDM, Der p 1 and Der p 2 by ELISA. Bronchoalveolar lavage (BAL fluid collected following allergen challenge was also assessed for the presence of HDM-specific antibodies. To examine the cellular immune response to HDM allergens, T cell proliferation and cutaneous responses were assessed in sensitized and control sheep. Results Strong HDM- and Der p 1-specific IgE, IgG1, IgG2 and IgA serum responses were observed in sensitized sheep, while detectable levels of HDM-specific IgG1 and IgA were seen in BAL fluid of allergen-challenged lungs. In contrast, minimal antibody reactivity was observed to Der p 2. Marked T cell proliferation and late phase cutaneous responses, accompanied by the recruitment of eosinophils, indicates the induction of a cellular and delayed-type hypersensitivity (DTH type II response by HDM and Der p 1 allergen, but not Der p 2. Conclusion This work characterizes the humoral and cellular immune effects of HDM extract and its major constituent allergens in sheep sensitized to HDM. The effects of allergen in HDM-sensitized sheep were detectable both locally and systemically, and probably mediated via enzymatic and immune actions of the

  13. House dust mite allergen Der f enhanced bronchial epithelial cell cytokine expression

    Institute of Scientific and Technical Information of China (English)

    BAO QING SUN; WEI TANG; ALBERT CHAN; ADRIAN WU; NAN SHAN ZHONG

    2006-01-01

    The house dust mites ( Dermatophagoides farinae, Der f) are the major source of aeroallergens implicated in the expression of atopic disorders, including asthma, allergic rhinitis and atopic dermatitis. In particular, strong circumstantial evidence suggests that house dust mite antigens are important precipitating factors of asthma. Many house dust mite allergens are proteases that can elicit airway inflammation by stimulating the release of cytokines from bronchial epithelial cells. To investigate whether Derf allergen proteases induced cytokine production from the epithelial cell line BEAS-2B,BEAS-2B cells were cultured with 4 different concentrations of Derf (0.02, 0.2, 2, 20 μg/ml) for 24-96 h, after which supernatants were assayed for interleukin (IL)-6 and IL-8 with ELISA. Reverse transcription-PCR was also performed. The cell sheets were intact throughout the observation in control group without any exposure to Derf antigen. In the experimental groups cells treated with Der f allergen showed changes in the anchorage status of the monolayer. There was a significant increase in the level of cytokine production compared with the untreated sample. The release of IL-6 and IL-8 increased in a concentration-dependent manner ( P < 0.05, respectively) with the addition of increasing dosage of Der f to the cell sheets. Levels of IL-6 and IL-8 began to rise at 24 h and 48 h after allergen exposure, and they increased significantly in the supernatants at 72 h and 96 h. At the same time the concentration dependence of induction of IL-6 and IL-8 expression as well as an increase in the expression of IL-6 and IL-8 mRNA manifested evidently. HDM-induced airway inflammation may include Der f-mediated release of inflammatory mediators, and the proteolytic activity of an allergen may stimulate the release of proinflammatory cytokines from human bronchial epithelium. It is suggested that IL-6 and IL-8 production by bronchial epithelial cells may play a role in the

  14. Block copolymer/DNA vaccination induces a strong allergen-specific local response in a mouse model of house dust mite asthma.

    Directory of Open Access Journals (Sweden)

    Camille Rolland-Debord

    Full Text Available BACKGROUND: Allergic asthma is caused by abnormal immunoreactivity against allergens such as house dust mites among which Dermatophagoides farinae (Der f is a common species. Currently, immunotherapy is based on allergen administration, which has variable effect from patient to patient and may cause serious side effects, principally the sustained risk of anaphylaxis. DNA vaccination is a promising approach by triggering a specific immune response with reduced allergenicity. OBJECTIVE: The aim of the study is to evaluate the effects of DNA immunization with Der f1 allergen specific DNA on allergic sensitization, inflammation and respiratory function in mice. METHODS: Mice were vaccinated 28 and 7 days before allergen exposure with a Der f1-encoding plasmid formulated with a block copolymer. Asthma was induced by skin sensitization followed by intra-nasal challenges with Der f extract. Total lung, broncho-alveolar lavage (BAL and spleen cells were analyzed by flow cytometry for their surface antigen and cytokine expression. Splenocytes and lung cell IFN-γ production by CD8+ cells in response to Der f CMH1-restricted peptides was assessed by ELISPOT. IgE, IgG1 and IgG2a were measured in serum by ELISA. Specific bronchial hyperresponsiveness was assessed by direct resistance measurements. RESULTS: Compared to animals vaccinated with an irrelevant plasmid, pVAX-Der f1 vaccination induced an increase of B cells in BAL, and an elevation of IL-10 and IFN-γ but also of IL-4, IL-13 and IL-17 producing CD4+ lymphocytes in lungs and of IL-4 and IL-5 in spleen. In response to CD8-restricted peptides an increase of IFN-γ was observed among lung cells. IgG2a levels non-specifically increased following block copolymer/DNA vaccination although IgE, IgG1 levels and airways resistances were not impacted. CONCLUSIONS & CLINICAL RELEVANCE: DNA vaccination using a plasmid coding for Der f1 formulated with the block copolymer 704 induces a specific immune response

  15. Oleanolic acid acetate inhibits atopic dermatitis and allergic contact dermatitis in a murine model

    Energy Technology Data Exchange (ETDEWEB)

    Choi, Jin Kyeong [CMRI, Department of Pharmacology, School of Medicine, Kyungpook National University, Daegu 700-422 (Korea, Republic of); Oh, Hyun-Mee [Bio-Materials Research Institute, Korea Research Institute of Bioscience and Biotechnology, Jeongeup 580-185 (Korea, Republic of); Lee, Soyoung [CMRI, Department of Pharmacology, School of Medicine, Kyungpook National University, Daegu 700-422 (Korea, Republic of); Park, Jin-Woo [Department of Periodontology, School of Dentistry, Kyungpook National University, Daegu 700-412 (Korea, Republic of); Khang, Dongwoo [School of Nano and Advanced Materials Science and Engineering, Gyeongsang National University, Jinju 660-701 (Korea, Republic of); Lee, Seung Woong; Lee, Woo Song [Bio-Materials Research Institute, Korea Research Institute of Bioscience and Biotechnology, Jeongeup 580-185 (Korea, Republic of); Rho, Mun-Chual, E-mail: rho-m@kribb.re.kr [Bio-Materials Research Institute, Korea Research Institute of Bioscience and Biotechnology, Jeongeup 580-185 (Korea, Republic of); Kim, Sang-Hyun, E-mail: shkim72@knu.ac.kr [CMRI, Department of Pharmacology, School of Medicine, Kyungpook National University, Daegu 700-422 (Korea, Republic of)

    2013-05-15

    Atopic dermatitis (AD) and allergic contact dermatitis (ACD) are common allergic and inflammatory skin diseases caused by a combination of eczema, scratching, pruritus, and cutaneous sensitization with allergens. This paper examines whether oleanolic acid acetate (OAA) modulates AD and ACD symptoms by using an existing AD model based on the repeated local exposure of mite extract (Dermatophagoides farinae extract, DFE) and 2,4-dinitrochlorobenzene to the ears of BALB/c mice. In addition, the paper uses a 2,4-dinitrofluorobenzene-sensitized local lymph node assay (LLNA) for the ACD model. The oral administration of OAA over a four-week period attenuated AD symptoms in terms of decreased skin lesions, epidermal thickness, the infiltration of immune cells (CD4{sup +} cells, eosinophils, and mast cells), and serum IgE, IgG2a, and histamine levels. The gene expression of Th1, Th2, Th17, and Th22 cytokines was reduced by OAA in the lymph node and ear tissue, and the LLNA verified that OAA suppressed ACD. The oral administration of OAA over a three-day period attenuated ACD symptoms in terms of ear thickness, lymphocyte proliferation, and serum IgG2a levels. The gene expression of Th1, Th2, and Th17 cytokines was reduced by OAA in the thymus and ear tissue. Finally, to define the underlying mechanism, this paper uses a TNF-α/IFN-γ-activated human keratinocyte (HaCaT) model. OAA inhibited the expression of cytokines and chemokines through the downregulation of NF-κB and MAPKs in HaCaT cells. Taken together, the results indicate that OAA inhibited AD and ACD symptoms, suggesting that OAA may be effective in treating allergic skin disorders. - Highlights: • OAA reduced both acute and chronic AD symptoms. • OAA had a controlling effect on the immune reaction for ACD. • The effect of OAA on allergic skin disorders was comparable to the cyclosporine A. • OAA might be a candidate for the treatment of allergic skin disorders.

  16. 过敏性紫癜患儿血清过敏原检测应用%Application of serum allergen detection for children with allergic purpura

    Institute of Scientific and Technical Information of China (English)

    曾子坤

    2016-01-01

    Objective To investigate clinical significance by serum allergen detection in diagnosis of children with allergic purpura. Methods A total of 120 children with allergic purpura received serum allergen detection of immune globulin E (IgE) antibody for allergen identification. Results The 120 cases had total positive rate of inhaled allergen as 54.17% (65 cases), with proportion as dermatophagoides farina > mould > tree pollen > house dust > hair and scurf of cat and dog > common ragweed artemisia, cockroach, mosquito. Their total positive rate of food allergen was 35.00% (42 cases), with proportion as peanut, soybean > shrimp, crab > pork, beef, mutton > egg > fish > milk. Conclusion Under certain circumstance, allergic purpura can be induced by various allergen. Therefore, both convention therapy and allergen detection are necessary in clinical treatment to identify allergen and restrain occurrence of allergic purpura.%目的:探究血清过敏原检测在过敏性紫癜患儿诊断中的临床意义。方法120例过敏性紫癜患儿,检测其血清中过敏原特异性免疫球蛋白 E(IgE)抗体等,并找出过敏原。结果120例患儿中,其中总吸入过敏原阳性率为54.17%(65例),过敏原占比由高到低排列为:尘粉螨>霉菌>树花粉>屋尘>猫狗毛发皮屑>普通豚草蒿属及蟑螂、蚊子;总食物过敏原阳性率为35.00%(42例),过敏原占比由高到低排列为:花生、大豆>虾、蟹>猪肉、牛肉、羊肉>蛋类>鱼>牛奶。结论多种过敏原在一定条件下均可诱发过敏性紫癜,因此,在临床治疗中既需要进行常规治疗,也应进行过敏原检测,以查找出过敏原,减少紫癜的发生。

  17. Prokaryotic expression and purification of recombinant products of group 2 house dust mite allergen%重组粉尘螨Ⅱ类变应原的原核表达及纯化

    Institute of Scientific and Technical Information of China (English)

    常维佳; 姜玉新; 陈文魁; 周书林

    2012-01-01

    Objectives To express and purify the cDNA of the group 2 allergen of Dermatophagoides farinae (Der f 2) and to determine the allergic activity of the resulting recombinant protein. Methods The Der f 2 gene was inserted into the expression vector pET-30a( + ) and transformed into competent E. Colt BL21 (DE3). The product induced by IPTG was analyzed with SDS-PAGE. The ability of Ig E to bind with sera from patients with an allergy to house dust mites was detected. Results SDSrPAGE testing showed that the amplified product with recombinant vector pET-30a-Der f 2 had a molecular mass of 15ku. This is consistent with the expected value. ELISA testing showed that the protein bound with the serum Ig E from patients with asthma. Conclusion Der f 2 has been successfully expressed in E. Colt and the purified recombinant protein has good allergenicity, providing a basis for animal testing of ASIT to treat allergy to house dust mites.%目的 表达并纯化粉尘螨2类变应原Der f 2蛋白,检测其变应原性. 方法 用1 mmol/L的IPTG诱导含重组质粒pET30a-Der f2的大肠埃希菌BL21( DE3),SDS-PAGE电泳检测并纯化目的蛋白,ELISA检测其与尘螨哮喘患者血清IgE的结合活性. 结果 SDS-PAGE电泳检测重组质粒pET30a-Der f 2表达产物,其分子质量单化为15 ku,与预期大小相符.ELISA检测纯化的Der f 2蛋白可与尘螨哮喘患者血清IgE反应. 结论 成功获得并纯化了Der f2重组蛋白,该蛋白具有变应原性,为进行尘螨过敏性疾病的变应原特异性免疫治疗的动物实验奠定了物质基础.

  18. Neonatal immune responses to TLR2 stimulation: Influence of maternal atopy on Foxp3 and IL-10 expression

    Directory of Open Access Journals (Sweden)

    Gold Diane R

    2006-03-01

    Full Text Available Abstract Background Maternal atopic background and stimulation of the adaptive immune system with allergen interact in the development of allergic disease. Stimulation of the innate immune system through microbial exposure, such as activation of the innate Toll-like-receptor 2 (TLR2, may reduce the development of allergy in childhood. However, little is known about the immunological effects of microbial stimulation on early immune responses and in association with maternal atopy. Methods We analyzed immune responses of cord blood mononuclear cells (CBMC from 50 healthy neonates (31 non-atopic and 19 atopic mothers. Cells were stimulated with the TLR2 agonist peptidoglycan (Ppg or the allergen house dust mite Dermatophagoides farinae (Derf1, and results compared to unstimulated cells. We analyzed lymphocyte proliferation and cytokine secretion of CBMC. In addition, we assessed gene expression associated with T regulatory cells including the transcription factor Foxp3, the glucocorticoid-induced TNF receptor (GITR, and the cytotoxic lymphocyte antigen 4 (CTLA4. Lymphocyte proliferation was measured by 3H-Thymidine uptake, cytokine concentrations determined by ELISA, mRNA expression of T cell markers by real-time RT-PCR. Results Ppg stimulation induced primarily IL-10 cytokine production, in addition to IFN-γ, IL-13 and TNF-α secretion. GITR was increased following Ppg stimulation (p = 0.07. Ppg-induced IL-10 production and induction of Foxp3 were higher in CBMC without, than with maternal atopy (p = 0.04, p = 0.049. IL-10 production was highly correlated with increased expression of Foxp3 (r = 0.53, p = 0.001, GITR (r = 0.47, p = 0.004 and CTLA4 (r = 0.49, p = 0.003, independent of maternal atopy. Conclusion TLR2 stimulation with Ppg induces IL-10 and genes associated with T regulatory cells, influenced by maternal atopy. Increased IL-10 and Foxp3 induction in CBMC of non-atopic compared to atopic mothers, may indicate an increased capacity to

  19. 上海嘉定地区儿童过敏原调查及过敏原与支气管哮喘的相关性%Allergens and their relationships to childhood bronchial asthma in the Jiading District of Shanghai

    Institute of Scientific and Technical Information of China (English)

    董蔚; 盛军; 顾秀明; 严华杰; 曾春云; 徐慧婷; 钱丹; 黄秋兰; 邵洁

    2012-01-01

    Objective To study allergens and their relationship to the occurrence of childhood bronchial asthma in the Jiading District of Shanghai. Methods Three hundred and eighty-two 4 to 12-year-old children with asthma in the remission stage from Nanxiang Hospital in the Jiading District of Shanghai were used as a case group (asthma group), and 402 children from two primary schools and two kindergartens in Jiading were enrolled by cluster sampling and served as control group. Parents of the children completed a questionnaire on living conditions and allergy-related disease history. Skin prick test ( SPT) for 18 common allergens was carried out in both groups. In order to examine the effect of environment and living conditions on SPT results, children in the control group were further divided into two sub-groups according to birth place: migrant (219 cases) and resident (183 cases). Results SPT results revealed that the main allergens identified in the Jiading region were dermatophagoides farinae, house dust mites, shrimps, cockroaches, and dog hair. The SPT positive rate was 67.9% in the asthma group, and this was significantly higher than in the control group (31.8%) (P<0.01). The environment and living conditions in the migrant group were significantly different from the resident group (P <0.01), whereas the SPT positive rate for this group was significantly lower than in the resident group (P < 0. 01). Conclusions Allergens in the Jiading region mainly originate from dermatophagoides farinae, household dust mites, shrimps, cockroaches and dog hair. Children with asthma are more susceptible to allergens. Environment and living conditions may be relevant, to a certain extent, to an SPT positive rate.%目的 分析上海嘉定地区过敏原及其与支气管哮喘发生的相关性.方法 上海嘉定南翔医院382例4-12岁缓解期支气管哮喘患儿作为病例组(哮喘组),采用整群随机法抽取上海嘉定地区2所学校和2所幼儿园402例4~10岁儿

  20. 过敏原皮肤点刺试验在成人慢性咳嗽病因诊断中的应用价值%Applying allergen skin prick test to etiological diagnosis of chronic cough in adult patients

    Institute of Scientific and Technical Information of China (English)

    丁慧强; 王莹; 陈亮

    2014-01-01

    目的:了解我院就诊的成人慢性咳嗽患者变应原分布情况及评价SPT的应用价值。方法:回顾性分析132例慢性咳嗽患者皮肤点刺试验结果,并比较不同性别、不同年龄段慢性咳嗽患者SPT阳性率结果。结果:①132例慢性咳嗽患者SPT结果阳性率为75.76%。不同性别慢性咳嗽患者SPT阳性率,经比较差异无统计学意义(χ2=0.530,P=0.466)。②14种过敏原中,SPT阳性率最高的为粉尘螨,户尘螨、蟑螂、花粉(包括蒿属花粉、葎草花粉、梧桐花粉、白桦花粉、玉米花粉)、真菌(包括酵母菌、青霉菌)、棉絮、猫毛、狗毛依次降低,最少为羽毛。尘螨点刺试验阳性率与其他过敏原相比,差异有高度统计学意义(χ2=715.334,P=0.000)。③不同年龄段慢性咳嗽患者SPT阳性率结果差异有统计学意义(χ2=10.305,P<0.05)。除中年人(45~59岁)与老年人(60~75岁) SPT阳性率之间的差异有统计学意义外,其余两两之间的差异均无统计学意义,可认为中年人SPT阳性率高于老年人。结论:粉尘螨和户尘螨是我院就诊的成人慢性咳嗽患者最常见的变应原,慢性咳嗽患者应尽早行过敏原皮肤点刺试验,其对慢性咳嗽患者的病因诊断有重要意义,并为舌下脱敏治疗提供临床依据。%Objective:To investigate the distribution of allergens in adult patients with chronic cough in Yijishan Hospital ,and evaluate the application value of skin prick testing (SPT).Methods:SPT results were retrospectively summarized in 132 adult cases of chronic cough,and positive SPTs were com-pared in genders and ages.Results:①Positive SPT was found in 75.76% of the 132 patients,yet there was no statistical significance in ages(χ2 =0.530, P=0.466);②Fourteen allergens detected via SPT stood from the highest to the lowest by Dermatophagoides farinae,house dust mites

  1. Cloning,Expression, Purification and Immunogenicity of House Dust Mite Allergen Der f5%粉尘螨Der f5克隆表达、纯化和免疫原性鉴定

    Institute of Scientific and Technical Information of China (English)

    袁小惠; 高安健; 邬玉兰; 刘志刚

    2013-01-01

    Objective To clone and express the antigen Der f5 from the dust mite dermatopha-goides farinae ,and to identify its immunogenicity. Methods A pair of primers were designed according to the known sequences of Der f 5 gene .Total RN A was extracted from dust mites and Der f5 gene fragments were amplified by RT-PCR .The PCR product was cloned into pMD18-T vector and transferred into E .coli Top10 .The clones were screened and identified by PCR and restriction enzyme digestion .The target gene obtained from the recombinant plasmid by digestion with Bam H Ⅰ and Hind Ⅲ was connected to the prokaryotic expression vector pET-32a.The expressed recombinant plasmid containing Der f5 gene was constructed by cloning target gene into pET-32a and transferred into E .coli Top10 .The recombinant plasmid was transfected into E .coli strain BL21 (DE3 ) and the transfected cells were grown in the presence of inducer IPTG .The expressed recombinant protein was analyzed by SDS-PAGE and Western blotting and was purified by immo-bilized metal ion affinity chromatography. Results The two recombinant plasmids ,pMD18-T-Der f5 and pET32a-Der f5,were constructed successfully .SDS-PAGE showed a correct molecular weight of the recombinant Der f 5 protein .After purification by affinity chromatography ,the protein showed only one strip on SDS-PAGE gel and appropriate ability to bind to IgE in sera of allergic patients .Conclusion The Der f5 gene has been cloned,expressed and purified and the recombinant Der f5 protein shows appropriate IgE-combined ability . The study provides a basis for specific diagnosis and treatment of dust mite-induced allergic disease.%目的 克隆表达粉尘螨第5组变应原(dermatophagoides farinae,Der f5)基因,并鉴定纯化蛋白免疫原性.方法 根据Der f5 基因已知序列,设计出相应的引物,提取粉尘螨总RNA,采用RT-PCR 方法扩增出Der f5 基因片段,PCR 产物克隆入pMD18-T 载体,转化大肠埃希菌 Top10,经PCR和酶切鉴定

  2. CLONING, EXPRESSION, PURIFICATION AND IMMUNOLOGICAL CHARACTERISTICS OF DER F18 FROM DUST HOUSE MITE DERMATOPHA GOIDES FARINAE%粉尘螨Der f18变应原的克隆表达、纯化及免疫学特性鉴定

    Institute of Scientific and Technical Information of China (English)

    朱永烽; 刘志刚; 高波

    2008-01-01

    通过对纯培养的粉尘螨提取总RNA,采用RT-PCR方法有效地扩增出Der f18片段,将Der f18连接到pET-24a表达载体上并转化到表达菌BL21(DE3)中,得到重组质粒pET-Der f18和重组工程菌.重组工程菌经IPTG诱导培养,高效表达出Der f18蛋白,SDS-PAGE结果显示表达产物约为52 kDa.表达产物经亲和层析纯化,用Western blot方法检测免疫学活性,结果表明目的蛋白具有良好的免疫原性.

  3. Acaricidal activity of Cymbopogon citratus and Azadirachta indica against house dust mites

    Directory of Open Access Journals (Sweden)

    Azima Laili Hanifah

    2011-10-01

    Conclusions: Generally, topical mortalities of D. farinae due to lemongrass are higher than that due to neem. Contact mortalities of lemongrass are always higher that neem against both species of mites.

  4. Download this PDF file

    African Journals Online (AJOL)

    Winnie

    worth and self-deprecation, stress related to body image, and personal control were also ... ducted qualitative investigations of mental health in adolescent males in California and has ... threat to society (Goffman, 1963; Jones, Farina, Hastorf.

  5. Guest Editorial The Obstetric Anaesthesia Special Interest Society ...

    African Journals Online (AJOL)

    , Z Farina, ... courses, educating the public on epidurals and pain relief in 30 languages ... The organisation will address interventions to improve outcomes, based primarily ... obstetric haemorrhage, and epidural and postoperative analgesia.

  6. The analysis of asthmatic bronchitis allergen skin prick test%喘息性支气管炎患儿皮肤过敏原点刺检测结果分析

    Institute of Scientific and Technical Information of China (English)

    姜君

    2013-01-01

    Objective To observe the allergen skin prick test for 12g patients with asthmatoid bronchitis to proceed early intervening treatment.Methods The dust mite allergen,dermatophagoides pteronyssinus 14 kinds of common allergen were detected in the 128 patients with pediatric asthmatoid bronchitis with the allergen skin prick test.According to age,the children were divided into two groups(more than 7 years old age group and under the age of seven group).Inhaled allergens (dust mites,household dust mites,the pollen of humulus scandens,dog hair,phoenix tree,etc.) and food allergens (eggs,milk,sea crab,scallops,etc.) were given in two groups.Afterwards the positive rate was analyzed.Results Among these 128 patients with pediatric asthmatoid bronchitis,the positive rate of allergen prick test result was 85.0% ; the inhaled allergens mid-point sting test result was 74.1% ; the food allergens was 10.8%.Conclusions The allergens can be found quickly and easily in the patients of pediatric asthmatoid bronchitis and the symptom severity of the patients of pediatric asthmatoid bronchitis can be effectively indicated with the allergen skin prick test.The skin prick test should be used widely in checking the allergens of pediatric asthmatoid bronchitis.%目的 探讨皮肤过敏原点刺试验检测喘息性支气管炎患儿临床效果.方法 对2012年1月1日至12月31日于我院就诊并确诊为喘息性支气管炎的128例患儿进行皮肤过敏原点刺试验,总点数为1792点,选取的过敏原为粉尘螨、户尘螨等14种常见的过敏原,给予患儿皮肤过敏原点刺,对吸入性过敏原(粉尘螨、户尘螨、葎草花粉、狗毛、梧桐等)及食物性过敏原(鸡蛋清、牛奶、海蟹、扇贝等)相关情况进行观察和过敏原阳性率等数据进行分析.结果 128例喘息性支气管炎患者过敏原点刺检测结果为阳性占85.0%(1523/1792),其中在吸入性过敏原中点刺检测结果阳性占74.1%(1328/1792),而食

  7. Chitins and chitosans as immunoadjuvants and non-allergenic drug carriers.

    Science.gov (United States)

    Muzzarelli, Riccardo A A

    2010-02-21

    Due to the fact that some individuals are allergic to crustaceans, the presumed relationship between allergy and the presence of chitin in crustaceans has been investigated. In vivo, chitin is part of complex structures with other organic and inorganic compounds: in arthropods chitin is covalently linked to proteins and tanned by quinones, in fungi it is covalently linked to glucans, while in bacteria chitin is diversely combined according to Gram(+/-) classification. On the other hand, isolated, purified chitin is a plain polysaccharide that, at the nano level, presents itself as a highly associated structure, recently refined in terms of regularity, nature of bonds, crystallinity degree and unusual colloidal behavior. Chitins and modified chitins exert a number of beneficial actions, i.e., (i) they stimulate macrophages by interacting with receptors on the macrophage surface that mediate the internalization of chitin particles to be degraded by lysozyme and N-acetyl-beta-glucosaminidase (such as Nod-like, Toll-like, lectin, Dectin-1, leukotriene 134 and mannose receptors); (ii) the macrophages produce cytokines and other compounds that confer non-specific host resistance against bacterial and viral infections, and anti-tumor activity; (iii) chitin is a strong Th1 adjuvant that up-regulates Th1 immunity induced by heat-killed Mycobacterium bovis, while down- regulating Th2 immunity induced by mycobacterial protein; (iv) direct intranasal application of chitin microparticles into the lung was also able to significantly down-regulate allergic response to Dermatophagoids pteronyssinus and Aspergillus fumigatus in a murine model of allergy; (v) chitin microparticles had a beneficial effect in preventing and treating histopathologic changes in the airways of asthmatic mice; (vi) authors support the fact that chitin depresses the development of adaptive type 2 allergic responses. Since the expression of chitinases, chitrotriosidase and chitinase-like proteins is greatly

  8. Chitins and Chitosans as Immunoadjuvants and Non-Allergenic Drug Carriers

    Directory of Open Access Journals (Sweden)

    Riccardo A. A. Muzzarelli

    2010-02-01

    Full Text Available Due to the fact that some individuals are allergic to crustaceans, the presumed relationship between allergy and the presence of chitin in crustaceans has been investigated. In vivo, chitin is part of complex structures with other organic and inorganic compounds: in arthropods chitin is covalently linked to proteins and tanned by quinones, in fungi it is covalently linked to glucans, while in bacteria chitin is diversely combined according to Gram(+/- classification. On the other hand, isolated, purified chitin is a plain polysaccharide that, at the nano level, presents itself as a highly associated structure, recently refined in terms of regularity, nature of bonds, crystallinity degree and unusual colloidal behavior. Chitins and modified chitins exert a number of beneficial actions, i.e., (i they stimulate macrophages by interacting with receptors on the macrophage surface that mediate the internalization of chitin particles to be degraded by lysozyme and N-acetyl-β-glucosaminidase (such as Nod-like, Toll-like, lectin, Dectin-1, leukotriene 134 and mannose receptors; (ii the macrophages produce cytokines and other compounds that confer non-specific host resistance against bacterial and viral infections, and anti-tumor activity; (iii chitin is a strong Th1 adjuvant that up-regulates Th1 immunity induced by heat-killed Mycobacterium bovis, while down- regulating Th2 immunity induced by mycobacterial protein; (iv direct intranasal application of chitin microparticles into the lung was also able to significantly down-regulate allergic response to Dermatophagoids pteronyssinus and Aspergillus fumigatus in a murine model of allergy; (v chitin microparticles had a beneficial effect in preventing and treating histopathologic changes in the airways of asthmatic mice; (vi authors support the fact that chitin depresses the development of adaptive type 2 allergic responses. Since the expression of chitinases, chitrotriosidase and chitinase-like proteins

  9. TBX21 and HLX1 polymorphisms influence cytokine secretion at birth.

    Directory of Open Access Journals (Sweden)

    Vera Isabel Casaca

    Full Text Available BACKGROUND: TBX21 (T cell specific T-box transcription factor and HLX1 (H.20-like homeobox 1 are crucial transcription factors of T(H1-cells, inducing their differentiation and suppressing T(H2 commitment, particularly important for early life immune development. This study investigated the influence of TBX21 and HLX1 single nucleotide polymorphisms (SNPs, which have previously been shown to be associated with asthma, on T(H1/T(H2 lineage cytokines at birth. METHODS AND FINDINGS: Cord blood mononuclear cells (CBMCs of 200 neonates were genotyped for two TBX21 and three HLX1 SNPs. CBMCs were stimulated with innate (Lipid A, LpA; Peptidoglycan, Ppg, adaptive stimuli (house dust mite Dermatophagoides pteronyssinus 1, Derp1 or mitogen (phytohemagglutinin, PHA. Cytokines, T-cells and mRNA expression of T(H1/T(H2-related genes were assessed. Atopic diseases during the first 3 years of life were assessed by questionnaire answered by the parents. Carriers of TBX21 promoter SNP rs17250932 and HLX1 promoter SNP rs2738751 showed reduced or trendwise reduced (p≤0.07 IL-5, IL-13 and TNF-α secretion after LpA-stimulation. Carriers of HLX1 SNP rs2738751 had lower IL-13 levels following Ppg-stimulation (p = 0.08. Carriers of HLX1 exon 1 SNP rs12141189 showed increased IL-5 (LpA, p = 0.007; Ppg, p = 0.10, trendwise increased IL-13 (LpA, higher GM-CSF (LpA/Ppg, p≤0.05 and trendwise decreased IFN-γ secretion (Derp1+LpA-stimulation, p = 0.1. Homozygous carriers of HLX1 promoter SNP rs3806325 showed increased IL-13 and IL-6 (unstimulated, p≤0.03. In carriers of TBX21 intron 3 SNP rs11079788 no differences in cytokine secretion were observed. mRNA expression of T(H1/T(H2-related genes partly correlated with cytokines at protein level. TBX21 SNP rs11079788 carriers developed less symptoms of atopic dermatitis at 3 years of age (p = 0.03. CONCLUSIONS: Polymorphisms in TBX21 and HLX1 influenced primarily IL-5 and IL-13 secretion after Lp

  10. Suppression of Immunotherapy on Group 2 Innate Lymphoid Cells in Allergic Rhinitis

    Institute of Scientific and Technical Information of China (English)

    Da-Chuan Fan; Xiang-Dong Wang; Cheng-Shuo Wang; Yang Wang; Fei-Fei Cao; Luo Zhang

    2016-01-01

    Background:Group 2 innate lymphoid cells (ILC2s) are regarded as a novel population of lineage-negative cells that induce innate Type 2 responses by producing the critical Th2-type cytokines interleukin (IL)-5 and IL-13.ILC2s as key players in the development of allergic rhinitis (AR) have been proved,however,the effect of subcutaneous immunotherapy (SCIT) with dermatophagoides pteronyssinus extract (Der p-SCIT) on ILC2s in AR patients is not clear.This study aimed to investigate the response of ILC2s of peripheral blood in house dust mites (HDM)-sensitized Chinese patients with AR who received SCIT with Der P extract.Methods:Seven healthy controls without symptoms of AR who had negative reactions to any of the allergens from skin-prick testing,nine patients diagnosed with persistent AR according to the Allergic Rhinitis and its Impact on Asthma (ARIA) guidelines,and 24 AR patients who received Der p-SCIT for 1.0-3.5 years were recruited for the study.ILC2s in the peripheral blood were evaluated using flow cytometry.The severity of their symptoms of all participants was rated based on the Total 5 symptom score.Results:Among 40 participants,9 AR patients were assigned to the untreated group,24 AR patients receiving Der p-SCIT were assigned to the immunotherapy group,and 7 healthy controls without symptoms of AR were assigned to healthy control group.The mean Total 5 symptom score of immunotherapy group was significantly lower than that of untreated group (4.3 ± 1.4 vs.10.1 ± 2.5,P < 0.001).Similarly,the levels of ILC2s in the peripheral blood of immunotherapy group were significantly reduced compared with that in untreated group (P < 0.001),but were not significantly different from healthy controls (P =0.775).Further subgroup analysis based on the duration of SCIT therapy (1.0-2.0 years [SCIT1-2],2.0-3.0 years [SCIT2-3],and 3.0-3.5 years [SCIT3-3.5]) showed that the percentage of ILC2s was not significantly different between SCIT1-2,SCIT2-3,and SCIT3

  11. 慢性荨麻疹患者血清中特异性 IgE 和 IgG检测的临床应用%Clinical application of serum specific IgE and IgG detection in chronic urticaria patients

    Institute of Scientific and Technical Information of China (English)

    何君; 邢艳

    2013-01-01

    目的:探讨慢性荨麻疹患者血清特异性IgE(sIgE)和特异性IgG(sIgG)检测的意义及其在临床中的应用。方法:对593例慢性荨麻疹患者采用免疫印迹法检测血清中食物过敏原和吸入性过敏原sIgE,同时采用酶联免疫法检测血清中食物sIgG,并在不同年龄组间进行比较。结果:593例慢性荨麻疹患者食物sIgG的总阳性率为76.73%,前4位的食物依次是鸡蛋、牛奶、蟹和大豆。食物sIgE的总阳性率为54.13%,前4位的食物依次是蟹、虾、牛奶和腰果;食物sIgG和sIgE的检测结果不一致( P<0.01)。不同年龄组的过敏原检测结果有所不同,吸入性过敏原sIgE的总阳性率为89.38%,前3位依次均是户尘螨、矮豚草、霉菌组合。结论:联合测定患者血清中sIgE和sIgG是寻找慢性荨麻疹患者过敏原的有效方法。尽早采取有效的环境控制和食物规避,对预防和治疗急性荨麻疹及慢性荨麻疹急性发作相当重要。%Objective:To investigate the significance of sIgE and sIgG detection and their clinical application in chronic urticaria serum patients .Methods:593 cases of chronic urticaria patients were recruited in this study .The food and inhalant sIgE were detected using immunoblot assay from serum of chronic urticaria patients , while enzyme-linked immunosorbent assay was applied for sIgG detection .The results in different age groups were compared .Results:The positive rate of ingested sIgG in 593 patients was 76.73%, the top four food was eggs , milk, crab and soybean .The ingested sIgE positive rate was 54.13%, and the top four food was crab , shrimp, milk and cashew nuts .Food sIgG and sIgE test results were significantly different ( P<0.01) .The anaphylactogen in different age groups was not the same .The total positive rate of inhalation of allergen sIgE was 89.38%, the top three was dermatophagoides pteronyssinus , dwarf ragweed , and mold combination

  12. Preparation and identification of monoclonal antibodies against Cor h 1 allergen%平榛主要过敏原C0rh1单克隆抗体的制备与鉴定

    Institute of Scientific and Technical Information of China (English)

    赵郭存; 张强; 邹菊; 黄钟; 刘志刚

    2011-01-01

    目的 制备和鉴定鼠抗平榛主要变应原Cor h 1的单克隆抗体(Monoclonal Antibody,McAb).方法 用重组Cor h 1蛋白为免疫原,免疫Balb/c小鼠,取免疫小鼠脾细胞与小鼠骨髓瘤NS-1细胞融合.通过间接ELISA法筛选分泌特异性McAb的杂交瘤细胞.用杂交瘤细胞株诱导小鼠产生腹水,再用蛋白A亲和层析法纯化抗体.采用Ig类与亚类鉴定试剂盒鉴定该单克隆抗体的Ig亚型;通过间接ELISA、Western Blotting鉴定该McAbs的特性和交叉性.结果 获得4株可稳定分泌鼠抗平榛主要变应原Cor h l的McAbs,其Ig亚型均为IgGl,均具有良好的效价;ELISA和Western Blotting分析表明该4株单抗均能识别重组Cor h l蛋白,其中3株单抗能够识别天然平榛提取物.结论 成功制备了4株鼠抗平榛主要变应原Cor h 1的单克隆抗体,为建立平榛主要变应原Cor h 1检测及纯化方法 奠定了基础.%This stuffy ained to prepare monoclonal antibodies against Cor h 1 allergen from Dermatophagoides farinae and to characterize their properties. Balb/c mice were immunized with purified recombinant Cor h 1 allergen protein, and the splenocytes of the immunized mice were fused with NS-1 myeloma cells by hybridoma technique. The McAbs against Cor h 1 allergen were purified using affinity chromatography on immobilized Protein A, and identified by ELISA and Western blotting for the specificity, subtype, titers and cross-reactivity. Four hybridoma cell lines secreting McAbs against Cor h 1 allergen were obtained, which were determined as IgG1 subtype and possessed good ascites titers. The four McAbs against Cor h 1 allergen all recognized recombinant Cor h 1 allergen, and the three of them also recognized native extract from Corylus heterophylla. In our study, four monoclonal antibodies against Cor h 1 allergen have prepared successfully, which will facilitate the establishment of detection method for Cor h 1 allergen.

  13. Freqüência de positividade em teste cutâneo para aeroalérgenos Frequency of positive skin tests for airborne allergic agents

    Directory of Open Access Journals (Sweden)

    Ricardo Godinho

    2003-12-01

    frequency of positive skin tests for specific airborne allergic agents, we assessed medical records of 398 patients with nasal symptoms suggestive of allergy or other otolaryngologic complaints which required skin testing to aid in the diagnosis. RESULTS: The most frequently found allergic agents were house dust (74,9%, dermatophagoides farinae (58,4% and mold (36,5%, followed by dog hair (32,7%, grass (22,4%, cat hair (16,9%, feathers (16,4% and wool (15,6%. Only 3,5% were positive for lovebird feathers and 1,5% for flowers. 39 patients (9,8% had negative skin tests, 10 (2,5% had reactions to the solution extract and 8 (2% did not react to histamine. We did not have complications in performing the test. CONCLUSION: House dust was the most frequently found allergic agent. Skin tests must be performed based on the clinical history, and their results may guide us towards the correct diagnosis and treatment of nose diseases.

  14. An observation of the characteristics of allergens and curative effect for patients with chronic intractable eczema in Zhaoqing%肇庆地区慢性顽固性湿疹患者的过敏原特征及疗效观察

    Institute of Scientific and Technical Information of China (English)

    帅春海; 陈洁华

    2014-01-01

    Objective To explore the relations between various allergens in nature and the morbidity of chronic eczema so as to provide guidance for prevention and treatment for the patients, and carry out a statistical assessment towards the curative effect after the treatment. Methods Allergens of 274 patients with chronic eczema were tested via BIotray866 Auto Blot Processor produced in Shenzhen Rayto Life and Analytical Sciences Co.,Ltd. The curative effect was observed after desensitization treatment. 125 patients with chronic eczema were selected as a control group, and received regular treatment for eczema instead of the desensitization treatment. Results Positive rate of allergens in 274 patients with chronic eczema reached up to 79.56%(218 patients); among the allergens for patients with positive results, the positive rate of dermatophagoides farina ranked the first with 46 patients, and other allergens were seafood, cow's milk and sheep's milk. Some patients were allergic to multiple allergens. The patients received desensitization treatment and kept away from the allergens, and only 28 patients(12.84%) relapsed in a 1-year follow-up, showing a significantly curative effect compared with 72 relapsed patients(57.6%) in the control group. Conclusion For patients with chronic intractable eczema, causes of disease should be actively traced during diagnosis and treatment, and related allergens should be avoided during their daily life. In such way, diseases can be effectively treated and prevented, and relapse rate can be significantly reduced.%目的:探讨自然界各种过敏原与慢性湿疹患者发病的关系,为患者的预防和治疗提供指导依据,并对治疗后的效果进行统计学评价。方法采用深圳雷杜BIotray866全自动过敏原分析仪器对274例慢性湿疹患者进行过敏原的检测,在脱敏治疗后观察疗效,同时设立125例对照湿疹患者,不进行脱敏治疗只进行常规湿疹治疗。结果274例慢性

  15. Study of clinical immunotherapy in children with allergic asthma and rhinitis%过敏性哮喘和鼻炎患儿的临床免疫治疗研究

    Institute of Scientific and Technical Information of China (English)

    杭杲; 刘春霞

    2014-01-01

    目的:探讨过敏性哮喘和鼻炎患儿的特异性免疫治疗的临床应用。方法采用体外特异性过敏原检测系统对儿童医院门诊患儿进行血清过敏原特异性 lgE(SlgE)抗体和总 lgE 进行检测,对尘螨过敏患儿进行分组治疗,免疫治疗组采用粉尘螨滴剂舌下脱敏治疗,对照组按普通药物抗过敏治疗,治疗6个月、12个月给予临床控制指标评分,同时观察患儿血清中 SIgE 的改变。结果218例过敏患儿吸入性过敏114例(52.4%),食入性过敏101例(46.3%)。98例尘螨过敏患儿占总过敏者和吸入性过敏者的44.95%和85.96%。免疫治疗组与对照组比较差异有统计学意义(P <0.05);脱敏治疗患儿 SIgE 阳性率有所下降,但差异无统计学意义(P >0.05)。结论218例过敏患儿的特异性过敏原检测中尘螨过敏者最多;舌下特异性免疫治疗过敏性哮喘和鼻炎效果显著。%Objective To investigate the clinical application of specific allergen immunotherapy (AIT)for the children with al-lergic asthma and rhinitis.Methods The in vitro specific allergen test system was adopted to detect allergen specific IgE and total IgE in children outpatients of the Children's Hospital.The children patients with dust mite allergy were divided into groups for con-ducting therapy.The immunotherapy group adopted the Dermatophagoides farinae drops for hypoglossis desensitization therapy, while the control group was performed the anti-allergic therapy by the common drugs.The clinical control indicator scores were per-formed after 6-months and 12-month therapy and at the same time the change of serum specific LgE was observed.Results Among 218 cases of allergy,114 cases(52.4%)were inhalation allergy and 101 cases (46.3%)were food allergy.98 cases of dust mite al-lergy accounted for 44.95% of the total allergy and 85.96% of the inhalation allergy.The difference in the curative effects

  16. 四川宜宾、云南昭通及部分长江上游地区变应性鼻炎患者的变应原分布特点及结果分析%Distribution characteristics of allergens in patients with allergic rhinitis in Yibin, Zhaotong and some areas of the Changjiang River

    Institute of Scientific and Technical Information of China (English)

    王建洪; 黄榆岚; 王仁君; 李雪梅; 杨红梅; 万林

    2016-01-01

    目的:通过对四川宜宾、云南昭通及部分长江上游地区变应性鼻炎患者行变应原检测及分析,筛查本地区常见变应原,了解常见吸入性变应原的分布情况,为临床诊断和治疗变应性鼻炎提供参考。方法采集2013年3月~2015年6月于四川省宜宾市第一人民医院就诊的661例经皮肤点刺试验(skin prick test,SPT)诊断明确的常年性变应性鼻炎患者的资料,对所测定的致敏变应原进行统计分析。结果在661例患者的SPT中,阳性率最高前5位为粉尘螨(76.55%)、屋尘螨(72.01%)、蟑螂(35.85%)、猫毛(11.35%)、狗毛(8.62%);不同季节变应原阳性率差异无统计学意义(χ2=4.568,P=0.206);随着年龄的增加,变应性鼻炎患者变应原阳性率呈下降趋势。结论四川宜宾、云南昭通及部分长江上游地区中导致变应性鼻炎的变应原占主导地位的为粉尘螨、屋尘螨、蟑螂,对于儿童的变应性鼻炎防控应尤为重视。%OBJECTIVETo screen the allergens in patients with allergic rhinitis (AR) in Yibin, Zhaotong and some areas of the Changjiang River, and to study the distribution characteristics of inhaled allergens. METHODSThe clinical data of 661 patients with allergic rhinitis who diagnosed by skin prick test from March 2013 to June 2015 in the First people's Hospital of Yibin City were studied.RESULTSIn 661 patients, the top 5 allergens and their positive rates of skin prick test were Dermatophagoides farinae (76.5%), house dust mites (72.01%), Roach (35.85%), cat hair (11.35%) and dog hair (8.62%) respectively. There were no statistical difference of the positive rates of allergens among different seasons (χ2=4.568, 0.206). With the increasing age of the patients, the positive rate of allergens was decreased. CONCLUSION The dominated allergens in patients with AR are dust mites, house dust mites and cockroaches in Yibin, Zhaotong and and some areas of the

  17. Cloning, Expression, Purification and Identification of Der f6 Gene and its Immunological Characteristics from the Dust House Mite%粉尘螨6类变应原(Der f6)的克隆表达、纯化及免疫学特性鉴定

    Institute of Scientific and Technical Information of China (English)

    朱永烽; 刘志刚; 高波

    2006-01-01

    目的 构建粉尘螨6类变应原(Dermatophagoides farinae,Der f6)基因的高效原核表达载体,并进行表达、纯化及生物学功能分析.方法 根据Der f6基因已知序列,设计1对引物,通过对纯培养的粉尘螨提取总RNA,采用RT-PCR方法扩增出Der f6基因片段,PCR产物克隆入pMD18-T载体,转化大肠埃希菌(E.coli Top10),经PCR和酶切鉴定并测序.将上述所得阳性克隆菌株扩大培养,碱裂解法提取质粒,所得重组质粒pMD18-T-Der f6和空质粒pET-24a同时用限制性内切酶EcoR Ⅰ和Xho Ⅰ双酶切,经纯化后连接并转化至E.coli Top10.构建的重组质粒pET24a-Der f6经PCR、酶切和测序鉴定后,再转化至E.coli BL21(DE3),异丙基-β-D-硫代半乳糖苷(IPTG)诱导表达.用十二烷基磺酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)和蛋白质印迹法(Western blotting)鉴定其表达效果,用Ni+离子亲和层析柱纯化重组质粒pET24a-Der f6表达产生的组氨酸重组蛋白.结果 构建了重组质粒pMD18-T-Der f6和pET24a-Der f6.SDS-PAGE结果表明Der f6基因在E.coli Bl21(DE3)中获得良好的表达,所得重组蛋白相对分子质量(Mr)为31 000,与理论值一致,经亲和层析纯化后,SDS-PAGE结果显示单一条带.该蛋白以尘螨过敏患者血清进行Western blotting,结果表明具有良好的IgE结合活性.结论 克隆、表达并纯化了具有良好尘螨致敏患者IgE结合活性的Der f6.

  18. The detection of inhalant allergens for children with asthma and the analysis of clinical features%哮喘患儿吸入性过敏原检测及其临床特征分析

    Institute of Scientific and Technical Information of China (English)

    成胜权; 强欢; 罗建峰; 丁翠玲; 丁亚楠

    2014-01-01

    Objective This study is aimed to investigate the type and distribution features of allergens inhaled by asthma children as well as their relationship with the age and gender of patients, to provide theoretical basis for the clinical diagnosis, treatment, and prevention of asthma in children. Method 13 types of standardized allergen skin prick liquid, provided by the Danish ALK-ABELL company, were used for inhaled allergen skin prick test (SPT) in 653 cases of children with asthma, treated in the pediatric clinic from July 2008 to July 2013. The patients were divided into three groups by age, <4 years old, 4-7 years old, and 7 -14 years old. The SPT-positive rate and changes of allergens species were compared among these three groups of patients. Result (1)Of the 653 patients,409(62.6%)cases were SPT-positive. The most prevalent inhalant allergens were: Dermatophagns farina in 266 cases(40.7%) , Dermatophagoides pteronyss inus in 265 cases (40.6%), tropical mite in 162 cases (24.8%), cat dander in 91 cases (13.9%), mugwort in 89 cases (13.6%);(2)The SPT-positive rate in males(299 in 455 cases; 65.7%) was greater than that in females (110 in 198 cases; 55.6%; P<0.05)The top 5 allergens were different between males and females. For males, they are house dust mites, dust mites, tropical mites, mugwort, and dog hair allergens, respectively. And for females, the top five allergens were dust mites, house dust mites, tropical mites, mugwort, and cat hair, respectively. (3)For <4 years old patients, the female's SPT-positive rate was higher than male's whereas male's SPT-positive rate was higher than females in groups of 4-7 years old and 7-14 years old. (4) SPT-positive male children is easier than SPT-positive female children with asthma to inhale three or more kinds of allergens(62.6% in male vs. 50.9% in female; P<0.05). Conclusion There are differences of gender and age in common inhalant allergens for children with asthma The top four common allergens were

  19. Analysis of allergens in 1088 patients accepting skin prick tests in the border region of Guangdong and Guangxi%两广交界地区过敏原皮肤点刺试验1088例结果分析

    Institute of Scientific and Technical Information of China (English)

    陈迪; 吴舜

    2013-01-01

    目的 了解广东与广西交界地区常见过敏原的分布情况和特点,为预防和治疗过敏性疾病提供指导意见.方法 采用粉尘螨皮肤点刺试剂对两广交界地区的1 088例过敏性疾病患者进行皮肤过敏原点刺检测.结果 1088例患者均完成皮肤点刺试验,总阳性率67.3% (732/1 088).4~15岁组皮肤点刺试验阳性率79.8% (213/267),16~30岁组阳性率为72.6% (201/277),31~45岁组的阳性率为57.3% (172/302),46~60岁组的阳性率为59.9% (145/242).4~30岁者的皮肤点刺试验阳性率高于31 ~60岁者(P<0.05).各年龄段患者的过敏原谱较一致,以粉尘螨(68.7%)和户尘螨(66.9%)为代表的尘螨是各过敏原中阳性率最高的,蟑螂(21.5%)次之;海虾(10.7%)、海蟹(18.2%)等海产品过敏原阳性率相对较高.过敏性鼻炎合并哮喘或结膜炎的患者,与单纯过敏性鼻炎患者的过敏原谱相似.结论 两广交界地区过敏性疾病中以尘螨为最主要的室内过敏原;粉尘螨特异性免疫治疗合并2种或2种以上的多重过敏原过敏性疾病具有可行性;该地区人群由摄入海河鲜引起的致敏现象较常见.%Objective To investigate the distribution and characteristics of the common allergens in the border region of Guangdong and Guangxi,and provide guidance for the prevention and treatment of allergic diseases.Methods Skin prick tests of dermatophagoides farina were performed in 1088 cases with allergic diseases from the border region of Guangdong and Guangxi.Results Skin prick tests were successfully performed in all 1088 patients.The total positive rate was 67.3% (763/1088).The positive rates in 4 ~ 15,16~30,31 ~45,46 ~60 years old group,were 79.8% (213/267),72.6% (201/277),57.3% (172/302),59.9% (145/242),respectively.The highest positive rate of allergens is the dust mites represented by the farinae and the house dust mites (68.7%,66.9%),followed by cockroach (21.5

  20. 哮喘患儿变应原特异性免疫球蛋白E与总免疫球蛋白E相关性研究%Relationship between total and specific IgE in patients with asthma.

    Institute of Scientific and Technical Information of China (English)

    王伟; 王芳; 刘哲伟

    2011-01-01

    目的 探讨哮喘患儿血清总免疫球蛋白E(IgE)及特异性免疫球蛋白E(SIgE)之间的关系,验证血清总IgE(TIgE)水平是否直接依赖于环境中占优势的变应原所诱导的SIgE(SIgE)升高而升高的观点.方法 收集2007年1月至2010年6月首都儿科研究所附属北京儿童医院门诊及住院的哮喘、咳嗽变异性哮喘(CVA)患儿219例,年龄3~16岁,平均(6.5±3.3)岁;其中男165例,女54例.应用荧光酶联免疫方法,进行混合真菌(mx1)、粉尘螨(d1)、户尘螨(d2)、猫毛(e1)、狗毛(e5)、艾蒿(w6)血清SIgE抗体和TIgE的测定.结果 219例患儿中SIgE阳性率分别是mx1:45.7%、d1:32%、d2:31.5%、e1:25.1%、e5:18.7%、w6:21.5%,中重度患儿的SIgE(≥50 U/mL)阳性比率是mx1:14.16%、d1:12.79%、d2:15.53%、e1:8.22%、e5:1.14%、w6:2.74%,(1)mx1、d1、d2、e1、e5、w6 血清SIgE抗体对TIgE的升高有显著意义,均为(r=0.000,P<0.01).(2)本值是以200为分界值,以TIgE≥200 U/mL 的病例为一组值进行分析,在TI gE≥200 U/mL者中特异性e1及特异性e5 IgE抗体明显导致TIgE的升高(P<0.01),而d2、d1、w6与高TIgE无相关性(P>0.01).结论 环境中占优势的过敏原并不都是可以导致较高的TIgE水平.%Objective To investigate the relationship between total immunoglobulin E (tlgE) and specific immunoglob- ulin E (slgE) in serum, and verify whether the elevation of serum tlgE directly depends on increasing slgE against the dominant allergen in the environment. Methods Totally 219 out-patients or in-patients with asthma or cough variant asthma(CVA) were selected randomly in our hospital from January 2007 to June 2010. There werel65 male and 54 female patients, with age ranging from 3 to 16 (mean : 6.5). With blood samples, tlgE and slgE directly against mixed molds ( mx 1), dermatophagoides ptemonyssinus (d 1), dermatophagoides farinae (d2 ), cat (el), dog (e5), and artemisia jvulgaris (w6) respectively were assayed by

  1. Clinical analysis of lnhaled al ergens in 579 children with al ergic rhinitis in Guangzhou%广州地区579例儿童过敏性鼻炎患者吸入过敏原的临床分析

    Institute of Scientific and Technical Information of China (English)

    张大威; 丘小汕; 何健荣; 刘立

    2015-01-01

    目的:通过皮肤点刺试验,考察广州地区过敏性鼻炎患儿吸入过敏原的种类及其分布,为诊断、预防和治疗儿童过敏性鼻炎提供参考。方法采用常见的11种吸入过敏原,对579例诊断为过敏性鼻炎患儿进行皮肤点刺试验,计算各种过敏原阳性率反应,并结合过敏原的种类、数量及患者年龄等临床资料进行分析。结果过敏原皮肤点刺试验阳性率为77.72%,以户尘螨、粉尘螨阳性率最高,分别为73.58%和74.78%,其次依次为狗毛>猫毛>德国小蠊>多支>霉菌Ⅱ>树 I >交链>杂草>葡萄球菌。只对单一类别的变应原呈阳性反应的有121例,占所有阳性例数的26.9%,其中主要为螨类,占107例。对两类变应原呈阳性反应的有261例,对三类或三类以上变应原呈阳性反应的有68例。此外,皮肤点刺阳性率与患者年龄相关(P<0.001),11岁~14岁儿童(86.5%)>7岁~10岁儿童(75.0%)>5岁~6岁儿童(64.1%)。结论分析结果提示户尘螨和粉尘螨阳性率显著高于其他过敏原,是广州地区儿童过敏性鼻炎最主要的吸入过敏原。过敏患者多数具有敏感体质,往往同时对多种过敏原产生过敏反应。值得注意的是皮肤点刺试验阳性率与患者年龄具有一定关系。%Objective Based on the study of the types and distribution of inhaled allergens in chil-dren with allergic rhinitis (AR) in Guangzhou by skin prick test (SPT), to provide reference for the diagnosis, treatment, and prevention of AR in children. Methods A total of 579 cases of children with AR were sub-jected to SPT with 11 common inhaled allergens. The positive rates of allergic reactions, the types of allergens, and the ages distribution were subjected to data analysis. Results Positive rate of skin prick test was 77.72%; house dust mite-positive and dermatophagoides farinae-positive were accounting for 73

  2. 77 FR 11414 - Incorporation by Reference

    Science.gov (United States)

    2012-02-27

    ..., identified using the subject line of this document, by any of the following methods: Federal eRulemaking... define only ``usability,'' and it does that for the pre- Internet age, in terms that plainly envision... College of Law Cynthia Farina Roberts Research Professor of Law Cornell Law School Tom Field Professor...

  3. 76 FR 52220 - Airworthiness Directives; Embraer-Empresa Brasileira de Aeronautica S.A. (EMBRAER) Model EMB-500...

    Science.gov (United States)

    2011-08-22

    ...-072-AD; Amendment 39-16779; AD 2011-17-15] RIN 2120-AA64 Airworthiness Directives; Embraer--Empresa... Empresa Brasileira de Aeron utica S.A., Phenom Maintenance Support, Av. Brig. Farina Lima, 2170, Sao Jose...--Empresa Brasileira de Aeronautica S.A.: Amendment 39-16779; Docket No. FAA-2011-0088;...

  4. Change of Th17 and expression of RORγt in a murine model of allergic rhinitis after a stimulation of allergen and corticosteroid%变应原和激素刺激后变应性鼻炎大鼠Th17细胞及RORγt表达变化

    Institute of Scientific and Technical Information of China (English)

    瞿申红; 李敏; 梁建平; 袁育林; 林志斌

    2011-01-01

    目的 了解变应原和激素对变应性鼻炎(AR)大鼠Th17细胞和孤独受体(RORγt)mRNA表达水平的影响.方法 以含2%氢氧化铝凝胶的屋尘螨提取液复制AR鼠模型后,将屋尘螨提取液对其中10只进行激发,10只进行经鼻吸入皮质类固醇[布地奈德(BUD)];10只用生理盐水雾化吸入.将处理后AR鼠模型和另10只对照鼠的脾脏细胞和鼻腔黏膜,用流式细胞术检测Th17细胞阳性率、逆转录聚合酶链反应检测RORγt mRNA表达水平、免疫组化检测鼠鼻腔黏膜RORγt表达.结果 AR鼠模型鼻黏膜中RORγt表达与正常对照组比较明显上调(25±5比48±10,P<0.05).而经BUD处理后AR鼠模型鼻黏膜中RORγt明显下调(48±10比31±6,P<0.05).AR鼠模型脾脏细胞中Th17细胞阳性率、RORγt mRNA的表达均高于对照组(18.4%±1.3%比27.5%±1.6%,0.43±0.04比0.64±0.05,均P<0.01).变应原激发后AR鼠模型脾脏Th17细胞阳性率和RORγt mRNA表达率分别显著低于处理前(27.5%±1.6%比20.0%±2.1%,0.64±0.05比0.54±0.03,均P<0.01);而BUD处理后二者变化不大(均P>0.05).结论 变应原疫苗影响变应性鼻炎机体全身免疫功能,可能的机制包括Th17细胞数下降、RORγtmRNA的表达下调来影响机体系统免疫功能;而局部激素对系统免疫作用不大,主要针对鼻黏膜局部免疫起作用,其机制包括RORγt表达下调.%Objective To study the effects of allergen and corticosteroid on T help 17 (Th17) and orphan nuclear receptor gammat (RORγt) in allergic-rhinitis mice.Methods Experimental allergic rhinitis (AR) was induced by the extract of dermatophagoides pteronyssinus (DP) including 2% ovalbumin (OVA)sensitization in 30 male mice with DP allergen group (n = 10),intranasal corticosteroid (budesonide,BUD) group (n = 10) or without treatment (model group,n = 10).And another 10 were included into the normal control group.The murine levels of Th17 were measured by flow cytometry (FCM).The expression of ROR

  5. Safety of specific immunotherapy with standardized house-dust mite vaccine in asthmatic children%标准化屋尘螨提取液治疗儿童支气管哮喘的安全性研究

    Institute of Scientific and Technical Information of China (English)

    郝创利; 陶慧; 沈美菊; 侯政

    2008-01-01

    asthma who were allergic to HDM received SIT with a standardized Dermatophagoides pteronyssinus(Der P)extract(Alutard SQ,ALK-Abell(o),Denmark).The local side effect and systemic side effect were monitored after each allergen injection.Results All patients completed a total of 2332 iniections.291 injections (12.48%)of all injections caused local side-effects,79 injections(3.39%)have sysmetic side effects.One injections caused severe(non-life-threatening)systemic reactions : symptoms:rapid onset(5 min after injection)of generalized urticaria,itching,flushing,severe asthma.In different dosage group:20-800 SQU,2 000-80 000 SQU,100 000 SQU,the 10cal side effect and systemic side effect were 1.05%,12.05%,19.62%and 0.35%,6.71%,2.51%,respectively.Local side effects were not correlated with systemic side effect.Conclusions Complying with the EAACI immunotherapy guidelines,SIT with standardized HDM vaccine is a safe treatment in asthmatic children.A majority of local reactions and all systemic reactions are mild and respond well to ordinary treatment and adjustment of dose-increase schedule.

  6. Establishment of ELISA techniques to detect Der f 1-specific IgE based on recombinant proteins%Der f1血清特异性IgE ELISA检测方法的建立

    Institute of Scientific and Technical Information of China (English)

    王运刚; 周鹰; 马桂芳; 杨李; 崔玉宝

    2012-01-01

    Objectives To establish an enzyme-linked immunosorbent assay (ELISA) technique to detect Der f 1-specific IgE based on recombinant proteins of group 1 allergens from Dermatophagoides farinae (rDer f 1) and to detect that IgE in sera from patients with asthma. Methods Indirect ELISA and ABC-ELISA techniques were developed using rDer f 1 as a capture antigen, and the techniques were then used to detect Der f 1-specific IgE in sera from 39 patients with asthma to analyze the sensitivity and specificity of the two techniques. Results With rDer f 1 as a coating antigen, the optimum concentration of coating antigen was 15 fig/ml and the proper dilution of serum was 1 : 2 for both indirect ELISA and ABC-ELISA to detect Der f 1-specific IgE. The proper dilution of horseradish peroxidase labeling anti-human IgE antibody (anti-human IgE/HRP) in indirect ELISA was 1 : 1000, and the proper dilution of anti-human IgE/Biotin and Avidin/HRP in ABC-ELISA was 1 : 1000 and 1 : 2000 , respectively. Indirect ELISA and ABC-ELISA had a sensitivity of 0. 68 U/ml and 0. 73 U/ml, respectively. Patients tested positive for Der f 1-specific IgE according to indirect ELISA and ABC-ELISA at rates of 92. 3% and 97. 4% respectively, and the rates did not differ significantly (P>0. 05). Conclusions Both ELISA techniques were highly sensitive at detecting rDer f 1 and should be used in laboratory diagnosis of allergic diseases associated with mites.%目的 利用粉尘螨变应原第1组分重组蛋白rDer f 1建立特异性IgE ELISA检测方法,并对哮喘患者血清进行检测.方法 以rDer f 1为包被抗原,分别建立间接ELISA法和亲和素(一)生物素复合ELISA (ABC-ELISA)法,检测Der f 1特异性IgE阳性标准血清和39例哮喘患者血清中的Der f 1特异性IgE,并分析检测结果.结果 间接ELISA法和ABC-ELISA法检测Der f 1特异性IgE的最适抗原包被浓度和血清稀释倍数均为15 μg/ml和1:2,间接ELISA法辣根过氧化物酶(HRP)标记抗人IgE

  7. [Ecological data on house dust mites in the city of Semipalatinsk].

    Science.gov (United States)

    Iagofarov, F F; Galikeev, Kh L

    1987-01-01

    Distribution of mites of the genus Dermatophagoides in flats of allergic patients depending on the type of the building structure, relative air humidity and season of the year was studied. The mites are most frequently encountered in houses of wood or adobe with high air humidity. Autumn is an optimum season for reproduction of mites.

  8. Estratégia e os Aspectos de Monitoramento/Controle nos Subsistemas Estritamente Coordenados

    Directory of Open Access Journals (Sweden)

    Pedro Henrique de Gois

    2012-06-01

    Full Text Available Este trabalho busca discutir a abordagem das estruturas estritamente coordenadas, arcabouço teórico desenvolvido por Zylbersztajn e Farina (1999 como uma perspectiva ampliada da firma, levando-se em conta as supply chains alimentícias como uma extensão do nexo de contratos proposto por Coase (1937 e retomada por Williamson (1985. As estruturas estritamente coordenadas, conforme destacam Zylbersztajn e Farina (1999, se voltam para identificação de pontos de interesses comuns que incitam as firmas a promoverem, entre si, contratos de maneira estritamente coordenados, considerando o grau de especificidade de ativos envolvidos na transação e as forças competitivas que determinam a busca de posicionamento estratégico pelas organizações para alcançarem resultados sustentáveis superiores.DOI:10.5585/riae.v11i1.1781

  9. [Human basophil degranulation test. Results of a modified technic (I)].

    Science.gov (United States)

    Román Pérez, F; Berlanga Cortés, J A; Urquiă, M; Guerra Pasadas, F; Peña Martínez, J

    1986-01-01

    The degranulation of human basophils towards antigenic substances, to which they must be previously sensitized as a result of the antigen-antibody union at the membrane level, is a phenomenon that has an important application complementing diagnosis of diseases mediated by the Gell and Coombs classification of Type I Hypersensitivity, on which the principle of Human Basophils Degranulation Test (HBDT) was based. An essential requirement is to be able to collect an adequate quantity of basophils, as well as the visualization by means of a technique of convenient stain. In this work, we show a modified HBDT technique for basophil granulocytes staining. A total of 33 HBDT was carried out on patients sensitive to grass pollen and/or Olea europea pollen and/or D. pteronyssinus, and as controls, HBDT was done on healthy individuals with negative clinical history. Basophil enrichemnt from the circulating blood sample was done by means of centrifugation in Percoll density gradients of 1072, 1076, 1080 and 1082, and we obtained a higher number of basophils with a density of 1080 at 400 Xg for 30 minutes. The cellular bottom was resuspended in 600 microliters of PBS. We employed lyophilized antigenic extracts from grass pollen, Olea europea and D. pteronyssinus, employing weight/volume concentration of 10(-4), 10(-5), 10(-6) and 10(-7). The optimum dilutions were 10(-5) for grass pollen and D. pteronyssinus.

  10. Knowledge-Based Solutions as they Apply to the General Radar Problem

    Science.gov (United States)

    2006-09-01

    systems form part of the subject of artificial intelligence, in which a knowledge base is used to guide an inference engine to make its processing...distributions, which are exactly the parts which have the greatest effect on the false alarm rate. Furthermore, high spatial resolution radars may be able to...Radar Data Procesing : Introduction and Tracking (vol.1), Research Studies Press, May 1985. 20. Farina, A., Antenna-based Signal Processing

  11. Rutin, quercetin, and free amino acid analysis in buckwheat (Fagopyrum) seeds from different locations.

    Science.gov (United States)

    Bai, C Z; Feng, M L; Hao, X L; Zhong, Q M; Tong, L G; Wang, Z H

    2015-12-29

    In this study, five common buckwheats and nine tartary buckwheats grown at different locations were analyzed for the contents of rutin, quercetin, and amino acids by high-performance liquid chromatography and spectrophotometry. The rutin content was higher than quercetin in buckwheat seeds. Rutin content was in the range from 0.05 (0.05 g per 100 g dry seeds) to 1.35% of buckwheat seeds. Quercetin content varied from 0.01 to 0.17% and in some common buckwheats it was even difficult to detect. Comparatively, tartary buckwheat seeds contained more rutin and quercetin than common buckwheat seeds. Meanwhile, the bran has higher rutin content than the farina in tartary buckwheat seeds, with a respective content of 0.45 to 1.19% and 0.14 to 0.67%. It was found that amino acid contents were around 1.79 to 12.65% (farina) and 5.74 to 7.89% (bran) in common buckwheats, and 1.73 to 5.63% (farina) and 2.64 to 16.78% (bran) in tartary buckwheat seeds. The highest total rutin content was found to be 1.35% in tartary buckwheat seeds from Sichuan, China. The highest total amounts of amino acid were detected to be 20.13% in tartary buckwheat seeds from Changzhi, Shanxi Province (China). Our results suggested that food products made of whole-buckwheat flour are healthier than those made of fine white flour.

  12. Aeroallergens in dairy barns near Cooperstown, New York and Rochester, Minnesota

    Energy Technology Data Exchange (ETDEWEB)

    Campbell, A.R.; Swanson, M.C.; Fernandez-Caldas, E.; Reed, C.E.; May, J.J.; Pratt, D.S. (Mayo Medical School, Rochester, MN (USA))

    1989-08-01

    We sampled atmospheric barn air using a volumetric air sampler in ten barns near Cooperstown, NY and six barns near Rochester, MN, and, with radioimmunoassays, measured allergens of Aspergillus fumigatus, Thermoactinomyces vulgaris, Micropolyspori faeni, short ragweed, rye grass group I pollen, Alternaria (Alt-1), Dermatophagoides sp. Lepidoglyphus destructor, common insect allergen, mouse urine, rat urine, and cattle epithelium. The most abundant allergen present was A. fumigatus followed by L. destructor. This study provides initial data on barn aerobiology and demonstrates for the first time the abundance of L. destructor allergens in North American dairy barns. More comprehensive study of barns, poultry houses, confinement houses for swine, and other agricultural environments from various geographic locations is needed to define the allergen levels to which millions of farm workers are exposed each day. While most of the allergens were expected, the presence of airborne allergens reactive with antisera to Dermatophagoides suggests indirectly that substantial amounts of pyroglyphid mites are present in some barns.

  13. Optimització de l'ús de la proteïna en dietes per a orada (Sparus aurata)

    OpenAIRE

    Caballero Solares, Albert

    2015-01-01

    [cat] Si vol consolidar-se com a explotació econòmicament i ecològicament sostenible, la piscicultura ha de deslligar-se de la pesca mitjançant la reducció del contingut de farina de peix als pinsos. Aquesta fita pot ser assolida ajustant la proporció de proteïna de la dieta als requeriments nutricionals dels peixos, o bé emprant fonts de proteïna vegetal. Per a optimitzar l'ús de la proteïna en dietes per a orada (Sparus aurata) es varen usar tres suplements alimentaris: el glutamat, la glu...

  14. Optimització de l'ús de la proteïna en dietes per a orada (Sparus aurata)

    OpenAIRE

    Caballero Solares, Albert

    2015-01-01

    Si vol consolidar-se com a explotació econòmicament i ecològicament sostenible, la piscicultura ha de deslligar-se de la pesca mitjançant la reducció del contingut de farina de peix als pinsos. Aquesta fita pot ser assolida ajustant la proporció de proteïna de la dieta als requeriments nutricionals dels peixos, o bé emprant fonts de proteïna vegetal. Per a optimitzar l'ús de la proteïna en dietes per a orada (Sparus aurata) es varen usar tres suplements alimentaris: el glutamat, la glutamina ...

  15. Severe Weather Guide Mediterranean Ports. 43. Sfax

    Science.gov (United States)

    1991-08-01

    POINTSE GUEURN BANZART DJEDIANE \\ /1 CAPE IiFARINA BIZERTE ’GULF OF GULF OF HANOMANIET 3N SOUSSE Li ALGERIA TUNISIA SFAX 5 0 KERKENNAH ISLANDS GULF OF 34-N...country. The adjacent Kerkennah Islands and the surrounding shallows offer excellent protection to the harbor and anchorage from both wind and seas. The...8217 3-1 Situated on the extreme north shore of the Gulf of Gabes, the Port of Sfax is protected to the east by the Kerkennah Islands, the nearest

  16. The Evaluation of Prick Test Results in Malatya

    Directory of Open Access Journals (Sweden)

    Hasan Kahraman

    2014-03-01

    Full Text Available Aim: Skin prick test used for determination of allergic diseases is an easy, cheap, safe, fast, and frequently utilized test. There are factors such as genetic tendency, climate, humidity, vegetation, and altitude affecting the development of allergy. Our goal is to determine the allergic susceptibility of allergic patient in Malatya province. Material and Method: The 403 patients who referred to our chest clinic in Malatya between January 2008 and June 2010 with complaint of respiratory allergic diseases including asthma and allergic rhinitis were retrospectively investigated. Skin prick test results were evaluated according to age and gender. Results: Of the 403 patients incorporated to the study, 177 (43.9% were male, 226 (56.1% were female. Most common reaction was against to grasses, in 78 patients (19.4%. The other common reactions were found against to grains, in 70 patients (17.4%; to D. Farinea in 51 patients (12.7%; to D Pteronyssinus in 44 patients (10.9%; to grass mix in 41 patients (10.2%. Although reactions were higher in men than in women, only against to D. Pteronyssinus was statistical significant (p=0.032. Reactions were observed most commonly in 10-19 age group and secondly in 20-29 age group. Discussion: Atopic patients in Malatya province should be careful especially against to grasses, grains, and house dust mites.

  17. Cedar Pollen Aggravates Atopic Dermatitis in Childhood Monozygotic Twin Patients with Allergic Rhino Conjunctivitis

    OpenAIRE

    Yukako Murakami; Saki Matsui; Akiko Kijima; Shun Kitaba; Hiroyuki Murota; Ichiro Katayama

    2011-01-01

    We report a case of 7-year-old monozygotic twin patients with atopic dermatitis. The HLA haplotypes were HLA A2, A11, B27, B61, DR1, and DR4. Both serum IgE levels and cedar pollen radioallergosorbent test (RAST) scores were high in the twins (elder/younger sister: IgE: 5170/3980 IU/ml and Japansese cedar pollen: >100/64.0) in contrast to low mite and food RAST scores (Dermatophagoides Pterygonium; 0.59/0.4 and egg white 9.24/4.6). The patients showed positive immediate (20 min in both sister...

  18. House-dust mites in our homes are a contamination from outdoor sources

    DEFF Research Database (Denmark)

    Hallas, Thorkil E.

    2010-01-01

    Avoidance advices for house-dust mite sensitized persons are currently based upon the idea, that the mites (Dermatophagoides spp.) are part of the indoor fauna. A closer look at development stages in the house-dust samples shows, however, that only the mites' active stages are present there and t...... no effect of avoidance measures. The verification of the entire hypothesis or part of it may have great impact on the management of the disease house-dust mite allergy.......Avoidance advices for house-dust mite sensitized persons are currently based upon the idea, that the mites (Dermatophagoides spp.) are part of the indoor fauna. A closer look at development stages in the house-dust samples shows, however, that only the mites' active stages are present...... there and that the stages between them, the inactive moulting stages, are absent. Therefore the mites probably do not carry out their life cycles in our dwellings, but are more likely contaminations from the open. Findings of low level concentrations can be explained by mites coming from outdoors and sedimented...

  19. EFFECTS OF SDS, PBS SOLUTIONS UPON FLUORESCENCE VALUES OF DERMATOPHA GOIDES PTERRONYSSINUS RADIOALLERGOSORBENT TEST INHIBITION

    Institute of Scientific and Technical Information of China (English)

    Jin-lu Sun; Hong-yu Zhang; Hai-juan He; Rui-qi Wang

    2005-01-01

    Objective To explore the effects of SDS, PBS re-dissolvent solutions on fluorescence values of radioallergosorbent test (RAST) inhibition.Methods Dermatophagoides pterronyssinus allergen immunoCAP and UniCAP 100 System were used. The Sera Pool consisted of 20 Dermatophagoides pterronyssinus allergic patients sera, their specific IgE fluorescence values were between 12 505 and 24 776.Results Fluorescence value percentages decreased: 62.9%, 54.1%, 43.5%, 6.7%, 3.7%, 2.6%, 2.2%, and 1.4%respectively, when SDS concentrations were at 2%, 1%, 0.5%, 0.25%, 0.1%, 0.05%, 0.025%, and 0.01%. Fluorescence values decreased more than 5% with SDS concentrations equal to 0.25% or higher. PBS in 0.1 and 0.01 mol/L concentrations decreased fluorescence values 2.9% and 0.9% respectively.Conclusions SDS is a commonly used surfactants in allergen extract and re-dissolvent prepared allergen precipitation for RAST inhibition. Thus effects of surfactants (e.g. SDS) upon the RAST inhibition tests must be considered when they were used as re-dissolvent agents to improve protein resolution in RAST inhibition.

  20. Sensitivity to the Main Allergens in Children with Allergic Diseases

    Directory of Open Access Journals (Sweden)

    O.D. Kuznietsova

    2016-01-01

    Objective of the research — to study hypersensitivity to the main allergens in children with allergic diseases based on the results of skin allergy testing, as well as to analyze the structure of diseases. Materials and methods. We have examined 228 children using skin prick testing, the estimation of results was conducted 25–40 minutes after performing the test. Associations between the results of skin prick test with various allergens were studied using cross-correlation analysis in the package of applied statistics Statistics 6.0. Results. 85.5 % of children were sensitized to the pollen allergens, domestic — 54 %, food — 21 %, fungal allergens — 35 %. Among pollen plants, there prevails sensitization to ambrosia — 47.8 %, sunflower — 49.5 %, cyclachaena — 38.5 %; among domestic allergens — to the tick species D.рteronyssinus and D.farinae — 24 %, cat hair — 19.7 %, among fungal — to Alternaria (23 %. Most often hyperergic reaction (papule diameter ≥ 8 mm was observed to cyclachaena (44 %, sunflower (46 %, ambrosia (50 %, cat hair (42 %, D.farinae (39 %. We have established significant (р < 0.05 correlations of mainly middle strength between positive prick-tests in pairs: ambrosia — cyclachaena (r = +0.43, ambrosia — sunflower (r = +0.43, acarus D.рteronyssinus — D.farinae (r = +0.66, mixture «birch, alder, oak, hazel» — ryegrass (r = +0.53, beef meat — egg yolk (r = +0.42, pork meat — chicken meat (r = +0.35, milk (r = +0.36, wool of sheep — pork (r = +0.36. Conclusion. Predominance of sensitization to pollen allergens represents the epidemiological situation in the South region of Ukraine. The presence of correlations between the different types of allergens indicates the cross reactions between them. In case of multiple positive results of skin allergen tests, the study using molecular allergy diagnostic method is recommended to establish genuine or cross allergy.

  1. Skin test reactivity among Danish children measured 15 years apart

    DEFF Research Database (Denmark)

    Thomsen, SF; Ulrik, Charlotte Suppli; Porsbjerg, C;

    2006-01-01

    test (SPT) positivity in Danish children has changed from 1986 to 2001. METHODS: Serial cross-sectional studies of two different random population samples of children aged 7 to 17 years of age, living in urban Copenhagen, Denmark, were performed 15 years apart. The first cohort was investigated in 1986...... (n = 527) and the second in 2001 (n = 480). Skin test reactivity to nine common aeroallergens was measured at both occasions. RESULTS: The prevalence of positive SPT to at least one allergen decreased from 24.1% in 1986 to 18.9% in 2001, (p = 0.05). We found a declining prevalence of sensitization...... to most allergens tested, statistically significant; however, only for mugwort and Alternaria iridis. Among subjects, who were sensitized to only one allergen, we found significantly fewer individuals with reactions to D. pteronyssinus and mugwort. CONCLUSIONS: The prevalence of atopic sensitization...

  2. Skin test reactivity among Danish children measured 15 years apart

    DEFF Research Database (Denmark)

    Thomsen, SF; Ulrik, Charlotte Suppli; Porsbjerg, C

    2006-01-01

    BACKGROUND: Knowledge of secular trends in the prevalence of allergy among children stems in large part from questionnaire surveys, whereas repeated cross-sectional studies using objective markers of atopic sensitization are sparse. OBJECTIVES: To investigate whether the prevalence of skin prick...... (n = 527) and the second in 2001 (n = 480). Skin test reactivity to nine common aeroallergens was measured at both occasions. RESULTS: The prevalence of positive SPT to at least one allergen decreased from 24.1% in 1986 to 18.9% in 2001, (p = 0.05). We found a declining prevalence of sensitization...... to most allergens tested, statistically significant; however, only for mugwort and Alternaria iridis. Among subjects, who were sensitized to only one allergen, we found significantly fewer individuals with reactions to D. pteronyssinus and mugwort. CONCLUSIONS: The prevalence of atopic sensitization...

  3. Distribution of arthropods in rice grains in Malaysia

    Institute of Scientific and Technical Information of China (English)

    Mariana A; Ho TM; Lau TY; Heah SK; Wong AL

    2009-01-01

    Objective:To determine distribution of arthropods in rice grains obtained from different sources.Methods:Rice samples were randomly collected from public in urban areas,farmers in rice field areas,aborigines in un-developed areas and retailers in commercial premises.Random samples of rice were taken out from each sam-ple for isolation of arthropods using a modified Berlese Tullgren Funnel Method.Mites were mounted prior to i-dentification;weevils were directly identified.Results:Samples of rice from retailers in commercial premises had the highest infestation by arthropods followed by samples from urbanites,aborigines and rice farmers.Two species of weevils,Sitophilus oryzae(S.oryzae)and Sitophilus granarius(S.granarius),were found.Samples from commercial premises had the least percentage of weevils compared to those collected from domestic premi-ses.Depending on the source of samples,densities of S.granarius and S.oryzae ranges from 1 1 -1 03 weevils? kg and 7-80 weevils?kg,respectively.Important species of mites in stored rice identified were mainly members of the families Cheyletidae,Echimyopodidae,Pyroglyphidae,Saproglyphidae and Tenuipalpidae.Among the species of mites identified were Austroglycyphagus malaysiensis,Cheyletus fortis,Cheyletus malaccensis,Der-matophagoides pteronyssinus,Grammolichus malukuensis and Suidasia pontifica.Average density of most of the mites was less than 40 mites?kg of rice grains.In this study,the highest number of mites in rice samples was recovered from commercial premises,followed by samples from urbanites.Samples from farmers and aborigines contained lesser mites.Conclusion:This study demonstrated the presence of 3 allergenic mite species in rice, i.e A.malaysiensis,D.pteronyssinus and S.pontifica.Weevils,S.oryzae and S.granarius that are known to be allergenic,were also found.

  4. Sviluppo e validazione di un antigene-capture ELISA basato su anticorpi monoclonali specifici per Listeria monocytogenes negli alimenti

    Directory of Open Access Journals (Sweden)

    Rossella Lelli

    2011-09-01

    Full Text Available È stato standardizzato e validato un dosaggio immunoenzimatico capture ELISA per l’identificazione di Listeria monocytogenes negli alimenti. Il dosaggio è stato messo a punto analizzando campioni di prodotti carnei, ittici e lattiero-caseari, pasta di semola e di farina di grano. Il metodo è risultato specifico al 100% per Listeria spp., con limite di rivelazione di 6,6 × 10(3 cfu/ml. Il metodo L. monocytogenes capture ELISA è stato confrontato con il metodo ufficiale ISO 11290-1:1996 per l’isolamento e l’identificazione di L. monocytogenes in matrici alimentari ottenendo un indice di concordanza significativo. Il dosaggio è stato validato in base alle indicazioni della norma ISO 16140:2003 relativamente ai metodi di analisi qualitativi. Il dosaggio è risultato accurato, specifico, sensibile, selettivo, riproducibile e rapido da eseguire, consentendo nello screening degli alimenti la riduzione di tempi e costi dell’indagine microbiologica.

  5. Secretome data from Trichoderma reesei and Aspergillus niger cultivated in submerged and sequential fermentation methods

    Directory of Open Access Journals (Sweden)

    Camila Florencio

    2016-09-01

    Full Text Available The cultivation procedure and the fungal strain applied for enzyme production may influence levels and profile of the proteins produced. The proteomic analysis data presented here provide critical information to compare proteins secreted by Trichoderma reesei and Aspergillus niger when cultivated through submerged and sequential fermentation processes, using steam-explosion sugarcane bagasse as inducer for enzyme production. The proteins were organized according to the families described in CAZy database as cellulases, hemicellulases, proteases/peptidases, cell-wall-protein, lipases, others (catalase, esterase, etc., glycoside hydrolases families, predicted and hypothetical proteins. Further detailed analysis of this data is provided in “Secretome analysis of Trichoderma reesei and Aspergillus niger cultivated by submerged and sequential fermentation process: enzyme production for sugarcane bagasse hydrolysis” C. Florencio, F.M. Cunha, A.C Badino, C.S. Farinas, E. Ximenes, M.R. Ladisch (2016 [1].

  6. Chapitre III. Tommaso Landolfi entre désir d’abandon et dégoût de l’informe

    OpenAIRE

    2016-01-01

    Il est difficile de lire ces quelques lignes, écrites par Giovanni Verga dans une lettre adressée à l’intellectuel Salvatore Farina, pour lui présenter sa nouvelle L’amante di Gramigna (publiée en 1880), exemple typique du récit vériste tel que Verga souhaitait en écrire, sans penser au mythe de Pygmalion ou au livre de Mary Shelley publié en 1818 : Frankenstein ou le Prométhée moderne. Ainsi est-on tenté de voir ici une sorte d’hybris romantique qui s’exprimerait sous la plume de ce parangon...

  7. Andrew Cole, The Birth of Theory

    Directory of Open Access Journals (Sweden)

    Corrado Confalonieri

    2016-06-01

    Full Text Available “Back to Hegel” è il titolo di un pezzo con cui Luca Illetterati presentava a fine 2014 su Alias (ma l’articolo è leggibile anche online, sul sito di hegelpd e sul blog Le parole e le cose le linee principali di due libri di Terry Pinkard allora da poco usciti in edizione italiana (La fenomenologia di Hegel: la socialità della ragione, a cura di Andrea Sartori e Italo Testa, traduzione di Andrea Sartori, Milano-Udine, Mimesis, 2013, e La filosofia tedesca 1760-1860: l’eredità dell’idealismo, a cura di Mario Farina, Torino, Einaudi, 2014 che costituiscono un nucleo importante del ritorno di interesse per la filosofia di Hegel verificatosi negli ultimi tre decenni in area nordamericana.

  8. Direct versus sequential immunoglobulin switch in allergy and antiviral responses.

    Science.gov (United States)

    Svirshchevskaya, E; Fattakhova, G; Khlgatian, S; Chudakov, D; Kashirina, E; Ryazantsev, D; Kotsareva, O; Zavriev, S

    2016-09-01

    Allergy is characterized by IgE production to innocuous antigens. The question whether the switch to IgE synthesis occurs via direct or sequential pathways is still unresolved. The aim of this work was to analyze the distribution of immunoglobulins (Ig) to house dust mite D. farinae and A. alternata fungus in allergic children with primarily established diagnosis and compare it to Epstein-Barr antiviral (EBV) response in the same patients. In allergy patients the only significant difference was found in allergen specific IgE, likely mediated by a direct isotype switch, while antiviral response was dominated by EBV specific IgG and low level of concordant IgA and IgG4 production consistent with a minor sequential Ig switches. Taken collectively, we concluded that sequential isotype switch is likely to be a much rarer event than a direct one.

  9. The hypocholesterolemic effects of beta-glucan in oatmeal and oat bran. A dose-controlled study.

    Science.gov (United States)

    Davidson, M H; Dugan, L D; Burns, J H; Bova, J; Story, K; Drennan, K B

    1991-04-10

    Oat cereals rich in the water-soluble fiber beta-glucan have been studied as a dietary therapy for hypercholesterolemia. To determine the hypocholesterolemic response of beta-glucan in the diet, 156 adults with low-density lipoprotein cholesterol (LDL-C) levels above 4.14 mmol/L (160 mg/dL) or between 3.37 and 4.14 mmol/L (130 and 160 mg/dL) with multiple risk factors were randomized to one of seven groups. Six groups received either oatmeal or oat bran at doses (dry weight) of 28 g (1 oz), 56 g (2 oz), and 84 g (3 oz). A seventh group received 28 g of farina (beta-glucan control). At week 6 of treatment, significant differences were found for both total cholesterol and LDL-C levels among the farina control and the treatment groups who were receiving 84 g of oatmeal, 56 g of oat bran, and 84 g of oat bran, with decreases in LDL-C levels of 10.1%, 15.9%, and 11.5%, respectively. Fifty-six grams of oat bran resulted in significantly greater reductions in LDL-C levels than 56 g of oatmeal. Nutrient analysis shows no difference in dietary fat content between these treatment groups; therefore, the higher beta-glucan content of oat bran most likely explains the significantly greater LDL-C reductions. A dose-dependent reduction in LDL-C levels with oat cereals supports the independent hypocholesterolemic effects of beta-glucan.

  10. Primary structure of Lep d I, the main Lepidoglyphus destructor allergen.

    Science.gov (United States)

    Varela, J; Ventas, P; Carreira, J; Barbas, J A; Gimenez-Gallego, G; Polo, F

    1994-10-01

    The most relevant allergen of the storage mite Lepidoglyphus destructor (Lep d I) has been characterized. Lep d I is a monomer protein of 13273 Da. The primary structure of Lep d I was determined by N-terminal Edman degradation and partially confirmed by cDNA sequencing. Sequence polymorphism was observed at six positions, with non-conservative substitutions in three of them. No potential N-glycosylation site was revealed by peptide sequencing. The 125-residue sequence of Lep d I shows approximately 40% identity (including the six cysteines) with the overlapping regions of group II allergens from the genus Dermatophagoides, which, however, do not share common allergenic epitopes with Lep d I.

  11. Bacterial derived proteoliposome for allergy vaccines.

    Science.gov (United States)

    Lastre, Miriam; Pérez, Oliver; Labrada, Alexis; Bidot, Igor; Pérez, Jorge; Bracho, Gustavo; del Campo, Judith; Pérez, Dainerys; Facenda, Elisa; Zayas, Caridad; Rodríguez, Claudio; Sierra, Gustavo

    2006-04-12

    One current approach in developing anti allergic vaccines is the use of potent adjuvants, capable of inducing Th1 or T regulatory cells. Proteoliposomes (PL) could be a suitable adjuvant. Purified Dermatophagoides siboney (Ds) allergens were mixed with PL and adsorbed into Al(OH)3 and evaluated in mice. The Th1/Th2 responses were measured at classes, subclasses, cytokines, and DTH levels. Anti Ds response was deviated to a Thl pattern, with the production of IgG2a and gamma1FN. A positive DTH response and a dramatic decrease of specific IgE and IL5 were not detected. The low dose was more effective than high dose. These results clearly support the potential use of PL as possible adjuvants for anti-allergic vaccines.

  12. [Aeroallergens, skin tests and allergic diseases in 1091 patients].

    Science.gov (United States)

    Enríquez Palomec, O; Hernández Chávez, L; Sarrazola Sanjuan, D M; Segura Méndez, N H; Hernández Colín, D D; Martínez-Cairo, S

    1997-01-01

    To know the frequency of positively of several skin tests, data cards from patients, of the Allergy and Clinic Immunology Service of the Hospital de Especialidades del Centro Medico Nacional Siglo XXI (Mexico City), between January, 1989 and March, 1995, were reviewed. Aqueous extracts manufactures by our laboratory were applied, in a dilution of 1:1000 weight-volume. 1091 from 5,651 skin tests patients were positive. Asthma and rhinitis were diagnosed in 492, allergic rhinitis in 289, allergic asthma in 111, and other diagnosis in 199 cases. The most frequent inhalable aeroallergens were house dust and perennial Dermatophagoides p and f1 with predominance in the rainy season, followed by pollens from Fraxinus a. Quercus a and Capriola, with predominance in the rainy season. The most frequent fungi were Candida and Fusarium, with predominance in the dry season.

  13. 粉螨变应原制备的实验研究进展

    Institute of Scientific and Technical Information of China (English)

    王晓春; 李朝品

    2006-01-01

    粉螨隶属于节肢动物门(Arthropoda)、蛛形纲(Arachnida)、蜱螨亚纲(Acari)、真螨目(Acariformes)、粉螨亚目(Acaridida),孳生于食品、药材、毛皮、房舍和储藏物中,与人类卫生保健关系密切。Voorhorst和Spieksrna于1964年报道了屋尘中的主要变应原来自于粉螨的分泌物、排泄物及其尸体的降解产物,其变应原活性与粉螨数量呈正相关。与变态反应性疾病关系最为密切的螨种包括腐食酪螨(Tyrophagus putrescentiae)、屋尘螨(Dermatophagoides pteronyssinus)、粉尘螨(Dermatophagoides farinae)、梅氏嗜霉螨(Euroglyphus maynei)及热带无爪螨(Blomia tropicalis)等,临床上可引起支气管哮喘、过敏性皮炎、过敏性鼻炎等疾病,因此,国内外对粉螨变应原的提纯和性质分析倍加重视。本文从粉螨天然变应原和重组变应原的制备纯化及其应用作一综述。

  14. Slope instability mapping around L'Aquila (Abruzzo, Italy) with Persistent Scatterers Interferometry from ERS, ENVISAT and RADARSAT datasets

    Science.gov (United States)

    Righini, Gaia; Del Conte, Sara; Cigna, Francesca; Casagli, Nicola

    2010-05-01

    In the last decade Persistent Scatterers Interferometry (PSI) was used in natural hazards investigations with significant results and it is considered a helpful tool in ground deformations detection and mapping (Berardino et. al., 2003; Colesanti et al., 2003; Colesanti & Wasowski, 2006; Hilley et al., 2004). In this work results of PSI processing were interpreted after the main seismic shock that affected the Abruzzo region (Central Italy) on 6th of April 2009, in order to carry out a slope instability mapping according to the requirement of National Department of Civil Protection and in the framework of the Landslides thematic services of the EU FP7 project ‘SAFER' (Services and Applications For Emergency Response - Grant Agreement n° 218802). The area of interest was chosen in almost 460 km2 around L'Aquila according the highest probability of reactivations of landslides which depends on the local geological conditions, on the epicenter location and on other seismic parameters (Keefer, 1984). The radar images datasets were collected in order to provide estimates of the mean yearly velocity referred to two distinct time intervals: historic ERS (1992-2000) and recent ENVISAT (2002-2009), RADARSAT (2003-2009); the ERS and RADARSAT images were processed by Tele-Rilevamento Europa (TRE) using PS-InSAR(TM) technique, while the ENVISAT images were processed by e-GEOS using PSP-DIFSAR technique. A pre-existing landslide inventory map was updated through the integration of conventional photo interpretation and the radar-interpretation chain, as defined by Farina et al. (2008) and reported in literature (Farina et al. 2006, Meisina et al. 2007, Pancioli et al., 2008; Righini et al., 2008, Casagli et al., 2008, Herrera et al., 2009). The data were analyzed and interpreted in Geographic Information System (GIS) environment. Main updates of the pre-existing landslides are focusing on the identification of new landslides, modification of boundaries through the spatial

  15. qPCR standard operating procedure for measuring microorganisms in dust from dwellings in large cohort studies.

    Science.gov (United States)

    Scherer, Emeline; Rocchi, Steffi; Reboux, Gabriel; Vandentorren, Stéphanie; Roussel, Sandrine; Vacheyrou, Mallory; Raherison, Chantal; Millon, Laurence

    2014-01-01

    The aim of the present study was to assess performance, feasibility and relevance of a Standard Operational Procedure (SOP) for large-scale use in the microbial analysis of children's indoor environments. We analyzed dust settled on Electrostatic Dust Fall Collectors (EDCs) by using qPCR which targeted 6 molds, 3 bacteria and 1 mite, chosen for their involvement in allergic or inflammatory processes. Six types of commercialized electrostatic wipes were tested for their releasing capacity of fungal DNA from fungal spores captured by the wipes. Specificity, repeatability and detection limits of the qPCR procedure were tested using calibrated microbial suspensions. The feasibility and relevance of this sampling and analysis method were assessed in a 75-home pilot study. Our result showed that one specific make of wipe was more effective than the others in releasing fungal DNA. qPCR procedure showed good repeatability. The quantification limit was about 5 fg DNA/μL for all species except Penicillium chrysogenum (0.5 fg DNA/μL) and Dermatophagoïdes pteronyssinus (10 fg DNA/μL). No cross-reactivity was observed. DNA concentrations in the 53/75 homes participating in the pilot study were between 0 and 24 625, 0 and 69 738 equivalent cells per cm(2) for the fungi and bacteria, and between 0 and 1 equivalent mites per cm(2) for D. pteronyssinus. Using the SOP described, we were able to classify the 53 dwellings from the least to the most contaminated according to the quantity of DNA measured for each species. Our SOP measured fungi, bacteria and mites using a cost-efficient, discreet and well-accepted sampling method with just one qPCR tool. The whole procedure can be used for microbial analysis in large cohort studies such as the ELFE study ("Etude Longitudinale Française depuis l'Enfance") and could help improve our understanding of the interactions between the environment, allergic diseases and child development.

  16. Impacto da tecnologia de informação no Supply Chain Management: um estudo multicaso sobre a adoção de EDI entre varejo e indústria agroalimentar The impact of information technology on the Supply Chain Management: a multicase study in EDI adoption between the agrifood industry and retailers

    Directory of Open Access Journals (Sweden)

    Andrea Lago da Silva

    1999-12-01

    Full Text Available As tecnologias de informação têm alterado as formas de coordenação entre os diferentes elos da cadeia de suprimentos. A coordenação pode ser entendida como a habilidade de transmitir informação, estímulos e controles ao longo das etapas seqüenciais que integram o conjunto de atividades necessárias para atender o mercado (FARINA & ZYLBERSZTAJN, 1994. O conjunto destas etapas é o que tem sido chamado de Supply Chain ou Cadeia de Suprimentos. Neste artigo, discute-se alguns casos de adoção de uma tecnologia de informação, o EDI (troca eletrônica de dados como ferramenta de coordenação entre varejistas e indústrias agroalimentares. Conclui-se que o processo de adoção é relativamente recente para que se possa mensurar resultados quantitativos e qualitativos significativos, mas já se observa alterações na freqüência de desabastecimento, em estoques melhor dimensionados e vendas maiores em decorrência da maior disponibilidade de mercadorias. Sinalizam-se também mudanças qualitativas, como alteração na função do vendedor tradicional, maior interação entre as áreas técnicas, com um aprendizado comum das novas formas de usar a tecnologia e o importante papel da confiança nos relacionamentos interorganizações.Information technology has changed the patterns of coordination among the different linkages of supply-chains. Coordination can be understood as the ability to transfer information, incentives and controls throughout the sequential stages which integrate the set of activities indispensable to serve the market (FARINA & ZYLBERSZTAJN, 1994. The sum of these stages is currently known as Supply-Chains. This paper discusses cases of adopting a specific information technology, EDI (Electronic Data Interchange, as an integration tool between the food industry and retailers. It was found that the adoption process is too recent to measure significative quantitative results, but it is possible to observe changes in the

  17. Enhanced Cysteinyl-Leukotriene Type 1 Receptor Expression in T Cells from House Dust Mite-Allergic Individuals following Stimulation with Der p

    Directory of Open Access Journals (Sweden)

    Maryse Thivierge

    2015-01-01

    Full Text Available In order to determine the potential for allergen to modulate T cell expression of the CysLT1 receptor and responsiveness to leukotrienes, peripheral blood mononuclear cells from house dust mite-allergic or nonallergic individuals were incubated with D. pteronyssinus allergen (Der p. Baseline CysLT1 expression was similar in both groups of donors, but Der p significantly enhanced CysLT1 expression in CD4+ and CD8+ T cells of only allergic individuals and induced enhanced responsiveness of CD4+ T cells to LTD4 in terms of calcium mobilisation. This effect was prevented by the CysLT1 antagonist MK571. Der p also induced IL-4 and IL-10 production, and neutralizing antibody to IL-4 prevented both the enhanced CysLT1 expression and the enhanced responsiveness of T cells to LTD4 induced by Der p. In allergic individuals, Der p also induced T cell proliferation and a Th2-biased phenotype. Our data suggest that, in allergen-sensitized individuals, exposure to allergen can enhance T cell expression of CysLT1 receptors through a mechanism involving IL-4 production. This, in turn, would induce CD4+ T cell responsiveness to cysteinyl-leukotrienes and Th2 cell activation.

  18. Purification and characterization of Lep d I, a major allergen from the mite Lepidoglyphus destructor.

    Science.gov (United States)

    Ventas, P; Carreira, J; Polo, F

    1992-04-01

    A major allergen of the storage mite Lepidoglyphus destructor (Lep d I) has been purified by affinity chromatography using an anti-Lep d I monoclonal antibody. The purity of the protein obtained by this procedure was assessed by reverse-phase HPLC. Lep d I displayed a molecular weight of 14 kD on SDS-PAGE under non-reducing conditions, and 16 kD in the presence of a reducing agent. Analytical IEF revealed a little charge microheterogeneity, showing three bands with pIs 7.6-7.8. Purified Lep d I retained IgE-binding ability, as proved by immunoblotting experiments after SDS-PAGE and RAST with individual sera from L. destructor-sensitive patients. Results from the latter technique demonstrated that 87% of L. destructor-allergic patients had specific IgE to Lep d I, and a good correlation between IgE reactivity with L. destructor extract and Lep d I was found. In addition, RAST inhibition experiments showed that IgE-binding sites on Lep d I are major L. destructor-allergenic determinants, since Lep d I could inhibit up to 75% the binding of specific IgE to L. destructor extract; on the other hand, Lep d I did not cross-react with D. pteronyssinus allergens.

  19. Recommendation of soil fertility levels for willow in the southern highlands of Santa Catarina

    Directory of Open Access Journals (Sweden)

    Tássio Dresch Rech

    2012-06-01

    Full Text Available The species Salix x rubens is being grown on the Southern Plateau of Santa Catarina since the 1940s, but so far the soil fertility requirements of the crop have not been assessed. This study is the first to evaluate the production profile of willow plantations in this region, based on the modified method of Summer & Farina (1986, for the recommendation of fertility levels for willow. By this method, based on the law of Minimum and of Maximum for willow production for the conditions on the Southern Plateau of Santa Catarina, the following ranges could be recommended: pH: 5.0-6.5; P: 12-89 mg dm-3; Mg: 3.2-7.5 mg; Zn: 5.0-8.3 mg dm-3; Cu: 0.8-4.6 mg dm-3; and Mn; 20-164 mg dm-3. The Ca/Mg ratio should be between 1.2 and 2.9. For K and Ca only the lower (sufficiency level, but not the upper threshold (excess was established, with respectively 114 mg dm-3 and 5.3 cmol c dm-3. It was also possible to determine the upper threshold for Al and the Al/Ca ratio, i.e., 1.7 cmol c dm-3 and 0.28, respectively. For maximum yields, the clay in the soil surface layer should be below 320 g dm-3.

  20. Evaluación de simulación de incapacidad laboral permanente mediante el Sistema de Evaluación Global (SEG

    Directory of Open Access Journals (Sweden)

    Mariana Abeledo

    2011-07-01

    Full Text Available Research on credibility and faking is essential in different areas of psychology and especially in the forensic field. The investigations performed by Arce and Farina are recognised as being among the most notable of all the existing investigations, with the development of the Global Evaluation System (GES that has been found to be especially effective in assessing the credibility of testimony and malingering mental health cases. The application of this procedure to 20 people accused under the judicial system of Social Security fraud for malingering a permanent work incapacity by way of a mental disorder, showed that the procedure described in the GES was effective for the correct classification of the maling