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Sample records for psychology cas dr

  1. OCCUPATIONAL HEALTH PSYCHOLOGY IN A CROSS-CULTURE PERSPECTIVE——An interview with Dr. Paul E. Spector

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    Occupational Health Psychology (OHP) is an interdisciplinary field concerned with psychological factors in employee's health, safety, and well-being, closely related to Industrial/Organizational (I/O) Psychology. Specific areas of concern are accidents, injuries, illness, stress, violence, counterproductive work behavior, and work-family conflict. Based on his research, Dr. Paul E. Spector answered our questions on aspects of Occupational Health Psychology, Cross-cultural Psychology and research/statistical methods in I/O Psychology. His opinions or suggestions about future research in I/O Psychology are informative and inspiring.

  2. [The effect of marriage on mental health. Dr. Djordje Natosević: psychological aspects of marriage].

    Science.gov (United States)

    Trećakov, D

    1994-01-01

    Dr. Dorde Natosević (1821-1887), our famous physician, health educator and reformer of the educational system, published an article "Marriage in regard to psyche" in the 111, book of the Chronical of Matica Srpska in 1866. In his article he consider the influence of both married and unmarried life on mental health of men and women. He gives advice: when, how and with whom one should and with whom one should not get married. His special interest were unmarried women and he gave numerous advice about making there lives more beautiful and interesting. Some of these references are actual even these days.

  3. CAS physicist receives Richard Geller Prize

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    @@ Dr. SUN Liangting from the CAS Institute of Modern Physics has been awarded the Richard Geller Prize for his outstanding contributions to the development of Electron Cyclotron Resonance (ECR) ion sources, especially an all-permanent magnetic one.

  4. Emerging Trends and Future Directions for the Field of Motivation Psychology: A Special Issue in Honor of Prof. Dr. Willy Lens

    Directory of Open Access Journals (Sweden)

    Maarten Vansteenkiste

    2016-07-01

    Full Text Available This special issue is devoted to Prof. Dr. W. Lens, who passed away end of August 2014 while he was vacationing. The special issue is meant to honor Willy’s intellectual contribution to the field of motivation psychology and his enthusiastic and devoted mentorship, which has spurred many of us to study motivation-related topics. In line with Willy’s interest and extensive network, the special issue brings together scholars from diverse theoretical perspectives (i.e., Achievement Goal Theory, Future Time Perspective Theory, and Self-Determination Theory and diverse cultural backgrounds (i.e., China, Peru, Greece, Portugal, Belgium, US, Australia, Canada. We introduce the special issue by highlighting four emerging trends that characterize contemporary motivation psychology and that were central to Willy’s work: (a multiperspectivism (i.e., a reliance on multiple motivational frameworks; (b the diversity of motives and goals that underlie behavior (i.e., motivational heterogeneity; (c interest in motives for non-participation; and (d the issue of universalism versus relativism (i.e., the question whether there exist universal motivational processes or whether these processes are contingent upon sociodemographic, personality-based, and contextual factors. Each of the eight contributions in the special issue touch upon one or more of these emerging themes, which are critically discussed in conjunction with a number of directions for future research.

  5. CAS Scientist Awarded Tyler Prize for Environment Achievement

    Institute of Scientific and Technical Information of China (English)

    Guo Haiyan; Zhao Baohua

    2002-01-01

    @@ Prof. Liu Dongsheng (Tungsheng Liu), a CAS member and professor at CAS Institute of Geology and Geophysics, has been awarded the 2002 Tyler Prize for Environment Achievement. He shared a cash prize of $200,000 with the other laureate, Dr. Wallace S. Broecker from Columbia University, the US, and received a gold Tyler Prize medallion on April 12.

  6. CAS announces new academicians

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    @@ Twenty-nine prominent Chinese scientists have been elected as CAS Members and five of their colleagues from US, France and Russia as CAS Foreign Members in 2007. The announcement was made by CAS Vice President LI Jinghai at a press conference held on 27 December, 2007 in Beijing. CAS President LU Yongxiang was present at the meeting.

  7. Psychology

    Science.gov (United States)

    Blackmore, Susan; Fouad, Nadya; Kagan, Jerome; Kosslyn, Stephen; Posner, Michael; Sternburg, Robert; Driscoll, Marcy; Ge, Xun; Parrish, Patrick

    2013-01-01

    Scholars representing the field of psychology were asked to identify what they considered to be the most exciting and imaginative work currently being done in their field, as well as how that work might change our understanding. The scholars included Susan Blackmore, Nadya Fouad, Jerome Kagan, Stephen Kosslyn, Michael Posner, and Robert Sternberg.…

  8. Psychology

    Science.gov (United States)

    Blackmore, Susan; Fouad, Nadya; Kagan, Jerome; Kosslyn, Stephen; Posner, Michael; Sternburg, Robert; Driscoll, Marcy; Ge, Xun; Parrish, Patrick

    2013-01-01

    Scholars representing the field of psychology were asked to identify what they considered to be the most exciting and imaginative work currently being done in their field, as well as how that work might change our understanding. The scholars included Susan Blackmore, Nadya Fouad, Jerome Kagan, Stephen Kosslyn, Michael Posner, and Robert Sternberg.…

  9. Large Customers (DR Sellers)

    Energy Technology Data Exchange (ETDEWEB)

    Kiliccot, Sila [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

    2011-10-25

    State of the large customers for demand response integration of solar and wind into electric grid; openADR; CAISO; DR as a pseudo generation; commercial and industrial DR strategies; California regulations

  10. [CAS General Standards 2012

    Science.gov (United States)

    Council for the Advancement of Standards in Higher Education, 2011

    2011-01-01

    The mission of the Council for the Advancement of Standards in Higher Education (CAS) is to promote the improvement of programs and services to enhance the quality of student learning and development. CAS is a consortium of professional associations who work collaboratively to develop and promulgate standards and guidelines and to encourage…

  11. A Tribute to Dr. Willy Lens

    Directory of Open Access Journals (Sweden)

    Maarten Vansteenkiste

    2016-07-01

    Full Text Available Dr. Willy Lens, born on December 10th, 1943, passed away on August 29th, 2014. With his passing, the motivation community has lost a seminal member, a mentor, and a friend. Dr. Lens – a Fellow of the Association for Psychological Science and Founding Fellow of the American Educational Research Association – made fundamental contributions to the study of motivation both through his own work and through his caring and thoughtful mentorship of a large community of scholars. With this tribute, we want to honor Dr. Willy Lens’ significance to psychology and education as well as his positive influence, both personally and professionally, on the lives of dozens of scholars. With his contagious enthusiasm and caring mentorship, Willy was an example for our academic community and with this tribute we express our gratitude for the privilege to have collaborated with him.

  12. Conference of CAS History Commission

    Institute of Scientific and Technical Information of China (English)

    2004-01-01

    @@ The Commission of CAS History held its third conference on Oct. 31 in Beijing. CAS Vice-President Yang Bailing and Vice-Secretary of the CPC Leading Group at CAS Guo Chuanjie addressed the meeting, which was chaired by Guo Huadong, CAS vice secretary -general.

  13. Research Highlights of CAS

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    @@ 1. Basic Research Basic research has always been one of the major tasks of CAS. Over the more than 50 years since its founding, CAS has gradually branched out to cover many fundamental fields of the natural sciences, such as mathematics, physics, chemistry, mechanics,astronomy, space science, life science, geoscience and environmental science - with nearly 10,000 research professionals and 117 open laboratories (including 50 key State laboratories). At present, there are 29 research institutes (including observatories) covering such fields as physical science, mathematics, mechanics and astronomy; with 31 open laboratories (nine key State laboratories, four State laboratories and 18 CAS open laboratories).

  14. Drømmejobbet

    DEFF Research Database (Denmark)

    Harrebye, Silas

    2012-01-01

    Medarbejdere vil i fremtiden også kunne arbejde, mens de sover. Virksomheder tilbyder snart deres ansatte interne kurser i ‘lucid dreaming’. Disse giver mulighed for, at man i sine drømme bliver bevidst om, at man drømmer og således kan manipulere dem. Det skal nu udnyttes. Management...

  15. Dr. Daniel Carter

    Science.gov (United States)

    1998-01-01

    Dr. Daniel Carter, president of New Century Pharmaceuticals in Huntsville, Al, is one of three principal investigators in NASA's microgravity protein crystal growth program. Dr. Carter's experties is in albumins. Albumins are proteins in the bloodstream that transport materials, drugs, nutrients, and wastes. Photo credit: NASA/Marshall Space Flight Center

  16. Dr. Daniel Carter

    Science.gov (United States)

    1998-01-01

    Dr. Daniel Carter, president of New Century Pharmaceuticals in Huntsville, Al, is one of three principal investigators in NASA's microgravity protein crystal growth program. Dr. Carter's experties is in albumins. Albumins are proteins in the bloodstream that transport materials, drugs, nutrients, and wastes. Photo credit: NASA/Marshall Space Flight Center

  17. Dr Math at your service

    CSIR Research Space (South Africa)

    Butgereit, L

    2012-10-01

    Full Text Available In this presentation the author explains how the Dr Math service works; how tutors are recruited to act as Dr Math; and how school pupils can reach Dr Math for help with their mathematics homework....

  18. Happy Birthday, Dr. Seuss!

    Science.gov (United States)

    Brodie, Carolyn S.

    2002-01-01

    Reports on the career of Theodor Seuss Geisel and suggests activities to celebrate his birthday. Lists selected children's books by Dr. Seuss, selected Seuss videos, Web sites, biographical resources, and biographical videos. (LRW)

  19. Dr.GEO

    Directory of Open Access Journals (Sweden)

    Francesca Campora

    2004-01-01

    Full Text Available Presentazione di Dr. Geo, un prodotto software Open Source progettato per lo studio interattivo della geometria euclidea piana e per un primo approccio ai fondamenti della programmazione in linguaggio Scheme.

  20. KODAK Directview DR Systems

    Directory of Open Access Journals (Sweden)

    Stefan Baum

    2007-08-01

    Full Text Available KODAK DIRECTVIEW DR 9500 System. The flexibility of a dual detector system in a unique, sin-gle detector design. The DR 9500 easily moves "around" the patient, making it ideal for all horizontal, upright and angled projections, and added convenience for patients - particularly those with limited mobility. "nKODAK DIRECTVIEW DR 7500 System New-generation system provides the ultimate in digital radiography flexibility. Modular components ena-ble you to easily configure a DR system to fit your space, applications, workflow - and budget."nHighest operator comfort:"n• Auto tracking (tube, table, wall stand, both direc-tions"n• Automatic, perpendicular centring to detectors center for table and wall stand"n• Auto positioning (choice of 16, customer programmable tube-detector positions"n• Manual or full motorised up and down motion and motorised detector tilt capability"n• Overhead tube system optional motorised, standard length rail system (5x3m"n• Floor mounted or with floor rails equipped DR 7500WM wall stand"n• Moveable, motorised table with floating top table"nScaleable: "n• degree of motorisation, degree of automatic func-tions"nKODAK DIRECTVIEW DR 3000 System This powerful multipurpose system is designed to handle a full range of general radiography procedures. With its simple design, space saving footprint, and advanced "ease of use" features, this system can enhance departmental workflow, productivity, and the quality of patient care.

  1. Asteroid named after CAS scientist

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    @@ An asteroid has been named after CAS astronomy historian XI Zezong with the approval of the International Minor Planet Nomenclature Committee (IMPNC), announced China's National Astronomical Observatories at CAS (NAOC) on 17 August.

  2. Cas9 Functionally Opens Chromatin.

    Directory of Open Access Journals (Sweden)

    Amira A Barkal

    Full Text Available Using a nuclease-dead Cas9 mutant, we show that Cas9 reproducibly induces chromatin accessibility at previously inaccessible genomic loci. Cas9 chromatin opening is sufficient to enable adjacent binding and transcriptional activation by the settler transcription factor retinoic acid receptor at previously unbound motifs. Thus, we demonstrate a new use for Cas9 in increasing surrounding chromatin accessibility to alter local transcription factor binding.

  3. CRISPR-Cas

    NARCIS (Netherlands)

    Jackson, Simon A.; McKenzie, Rebecca E.; Fagerlund, Robert D.; Kieper, Sebastian N.; Fineran, Peter C.; Brouns, Stan J.J.

    2017-01-01

    Bacteria and archaea are engaged in a constant arms race to defend against the ever-present threats of viruses and invasion by mobile genetic elements. The most flexible weapons in the prokaryotic defense arsenal are the CRISPR-Cas adaptive immune systems. These systems are capable of selective

  4. Drømmebilleder

    DEFF Research Database (Denmark)

    Fausing, Bent

    2014-01-01

    Bogen beskriver i en række intense analyser forbindelsen mellem ydre og indre billeder: altså mellem de moderne mediers billedverden og drømmens og erindringens indre billeder. I fokus for undersøgelsen er desuden kønnet, altså hvordan billeder viser, omformer eller skjuler kønnet, 'det andet', s...

  5. Drømmebilleder

    DEFF Research Database (Denmark)

    Fausing, Bent

    2014-01-01

    Bogen beskriver i en række intense analyser forbindelsen mellem ydre og indre billeder: altså mellem de moderne mediers billedverden og drømmens og erindringens indre billeder. I fokus for undersøgelsen er desuden kønnet, altså hvordan billeder viser, omformer eller skjuler kønnet, 'det andet', s...

  6. Young CAS biologist gets five-year grant from Howard Hughes Medical Institute

    Institute of Scientific and Technical Information of China (English)

    2012-01-01

    Dr. TANG Chun, an outstanding young biologist from the CAS Wuhan Institute of Physics and Mathematics (WIPM) was announced in January 2012 to have won the first International Early Career Award from Howard Hughes Medical Institute with a five-year grant totaling up to 650,000 US dollars. Dr. Tang is an expert in using novel nuclear magnetic resonance methods to study the behavior and function of proteins,

  7. CAS information services for medicinal chemists.

    Science.gov (United States)

    Heyman, J J; Karasinskia, E H; Giles, P M

    1982-01-01

    In summary, the medicinal chemist has four new ways to access the information contained in the CAS database: CAS ONLINE, the only structure search system based on the entire CAS Registry database, and three printed services, CA SELECTS, BIOSIS/CAS SELECTS, and CAS Customer-Defined Information Services, which provide abstracts on specialized topics of interest.

  8. CAS, INRA to Set Up Sino-French Lab for Mammal-embryo Cell-biology

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    @@ CAS Vice President CHEN Zhu and Dr. Marion Guillou, chairman of Institut National de la Recherche Agronomique (INRA) in France,signed on Nov. 4 in Beijing an agreement on the founding of a SinoFrench lab for mammal-embryo cell biology (LABIOCEM).

  9. Recent CAS Achievements in Life Sciences

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    @@ Recent years have witnessed remarkable progress scored by CAS researchers along with the smooth development of the knowledge innovation program piloted at CAS. The follow-ings are just recent examples of CAS research achievements in life sciences.

  10. Cas1–Cas2 complex formation mediates spacer acquisition during CRISPR–Cas adaptive immunity

    OpenAIRE

    Nuñez, James K.; Kranzusch, Philip J.; Noeske, Jonas; Wright, Addison V.; Davies, Christopher W; Doudna, Jennifer A.

    2014-01-01

    The initial stage of CRISPR–Cas immunity involves the acquisition of foreign DNA spacer segments into the host genomic CRISPR locus. The nucleases Cas1 and Cas2 are the only proteins conserved amongst all CRISPR–Cas systems, yet the molecular functions of these proteins during immunity are unknown. Here we show that Cas1 and Cas2 from Escherichia coli form a stable complex that is essential for spacer acquisition and determine the 2.3-Å resolution crystal structure of the Cas1–Cas2 complex. M...

  11. Dr. julio iribarne

    OpenAIRE

    Revista de la, Facultad de Medicina

    2011-01-01

    El Dr. lribarne fue profesor titular de Ginecología, dos veces Decano de la Facultad de Ciencias Médicas de Buenos Aires, uno de los profesores más ilustres, llamado el Decano de las reformas universitarias, escritor y sociólogo profundo conocidoy respetado en todos los centros científicos de Europa y del Nuevo Continente, director de la "Revista Médica Latino Americana". Lamentamos el fallecimiento de este colega que no solamente era una gloria del cuerpo médico argentino, sino de la América...

  12. Research Facility Development at CAS

    Institute of Scientific and Technical Information of China (English)

    Tian Dongsheng; Miao Yougui; Zhang Hongsong

    2005-01-01

    @@ This article gives an introductory account on the development of research facilities at the CAS over the past six years since the initiation of the Knowledge Innovation Program in 1998 and during the period of the national 10th Five-year Plan in particular. In addition, it expounds the key points for the future work at the CAS in this regard.

  13. CAS School in Germany

    CERN Multimedia

    CERN Accelerator School

    The CERN Accelerator School (CAS), the Helmholtz Centre for Heavy Ion Research GmbH (GSI) and the Technische Universität Darmstadt (TU Darmstadt) jointly organised a course on General Accelerator Physics, at intermediate level, at TU Darmstadt from 27 September to 9 October 2009.   Participants in the CERN Accelerator School in Darmstadt, Germany. The Intermediate-level course followed established practice, with lectures on core topics in the mornings and specialised courses in the afternoons. The latter provided "hands-on" education and experience in the three selected topics: "RF Measurement Techniques", "Beam Instrumentation and Diagnostics" and "Optics Design and Correction". These proved to be highly successful, with participants choosing one course and following the topic throughout the school. Guided studies, tutorials, seminars and a poster session completed the programme. A visit to GSI and the F...

  14. Dual nuclease activity of a Cas2 protein in CRISPR-Cas subtype I-B of Leptospira interrogans.

    Science.gov (United States)

    Dixit, Bhuvan; Ghosh, Karukriti Kaushik; Fernandes, Gary; Kumar, Pankaj; Gogoi, Prerana; Kumar, Manish

    2016-04-01

    Leptospira interrogans serovar Copenhageni strain Fiocruz L1-130 carries a set of cas genes associated with CRISPR-Cas subtype I-B. Herein, we report for the first time active transcription of a set of cas genes (cas1 to cas8) of L. interrogans where cas4, cas1, cas2 and cas6, cas3, cas8, cas7, cas5 are clustered together in two independent operons. As an initial step toward comprehensive understanding of CRISPR-Cas system in spirochete, the biochemical study of one of the core Leptospira Cas2 proteins (Lep_Cas2) showed nuclease activity on both DNA and RNA in a nonspecific manner. Additionally, unlike other known Cas2 proteins, Lep_Cas2 showed metal-independent RNase activity and preferential activity on RNA over DNA. These results provide insight for understanding Cas2 diversity existing in the prokaryotic adaptive immune system.

  15. Teaching Peace with Dr. Seuss.

    Science.gov (United States)

    Pace, Rosemarie; Podesta, Andrea

    1999-01-01

    Educators seeking novel ways to instill conflict-resolution skills in young children should consider Dr. Seuss, whose books provide a synthesis of fantasy and reality that works for teaching values endemic to peace education. This paper discusses how students can learn peace and educators can teach peace using Dr. Seuss books, examining steps to…

  16. From Mxit to Dr Math

    CSIR Research Space (South Africa)

    Botha, Adèle

    2013-02-01

    Full Text Available In 2007, Laurie Butgereit, a researcher at the CSIR Meraka Institute, started to use Mxit as a communication channel to tutor her son in mathematics. Her son and a number of his friends logged in, and Dr Math was born. At the inception of Dr Math...

  17. CAS Mathematician Receives Shaw Prize

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    @@ WU Wentsun (WU Wenjun), a CAS Member from the Academy of Mathematics and System Sciences, has been honored with the 2006 Shaw Prize in Mathematical Sciences for his contributions to the new interdisciplinary field of mathematics and mechanization.

  18. Dr Stanislaw Huskowski, Mayor of Wroclaw, Poland

    CERN Document Server

    Patrice Loïez

    2002-01-01

    Dr Stanislaw Huskowski, Mayor of Wroclaw, Poland visiting the ATLAS magnet assembly hall, building 180. From l to r: Mr Carlo Lamprecht, State Councillor, Dr Stanislaw Huskowski and Dr Peter Jenni, ATLAS Spokesperson

  19. Dr Stanislaw Huskowski, Mayor of Wroclaw, Poland

    CERN Multimedia

    Patrice Loïez

    2002-01-01

    Dr Stanislaw Huskowski, Mayor of Wroclaw, Poland visiting the ATLAS magnet assembly hall, building 180 with Mr Carlo Lamprecht, State Councillor, Dr Stanislaw Huskowski and Dr Peter Jenni, ATLAS Spokesperson

  20. Entrevista al Dr. Juan Marconi, Creador de la Psiquiatría Intracomunitaria: Reflexiones Acerca de su Legado Para la Psicología Comunitaria Chilena Interview With Dr. Juan Marconi, Intracommunity Psychiatry Program Creator: Reflections About his Legacy for Chilean Psychology Community

    Directory of Open Access Journals (Sweden)

    Susana Mendive

    2004-11-01

    Full Text Available El presente trabajo pretende rescatar el programa Psiquiatría Intracomunitaria desarrollado durante 1968 y 1973 en Santiago de Chile. Este programa fue un modelo de intervención con participación de la comunidad en alcoholismo, neurosis y privación sensorial. Una figura central en el diseño e implementación del mismo fue el psiquiatra Juan Marconi. Con la finalidad de rescatar aspectos no conocidos y reflexiones del programa es que este artículo se centra en el testimonio de quien encabezó esta iniciativa. Adicionalmente, considerando la dimensión histórica que adopta la publicación de este testimonio para la psicología comunitaria, en la segunda parte se incluye la percepción de dos destacados psicólogos comunitarios chilenos respecto a la contribución del programa a la disciplina, en su etapa de nacimiento.The present article has the pretence of rescuing the Intracommunity Psychiatry program developed between 1968 and 1973 in Santiago de Chile. This program became a model of interventions based on community participation in alcoholism, neurosis and sensorial deprivation situations. A central figure in its design and implementation was the psychiatrist Juan Marconi. With the aim of rescuing unknown topics and reflections based on this program, the present article focuses on the testimony of the person that lead this initiative. Additionally, considering the historical dimension that the publication of this testimony has for community psychology, the second part of this article includes the perception of two well known community psychologists about the contribution of this program to the discipline, in its initial phase.

  1. How Dr. Pierce Promoted Himself

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This article is about Dr. Raymond V Pierce who owned St. Vincent Island before it became a refuge. The doctor painted advertisements for his famous “Woman’s Tonic”...

  2. Dr. Millie Cleveland: chiropractic achiever.

    Science.gov (United States)

    Nash, J

    1998-06-01

    Dr. Mildred Genoa Allison-Cleveland, affectionately known as "Dr. Millie," was an important part of life at Cleveland Chiropractic College for over thirty years, serving first as a clerical assistant, then instructor, administrative assistant, and trusted right hand of her husband, Dr. Carl Cleveland, Jr. Yet Mildred Cleveland, like many other women who have helped the profession grow and survive over the years, has never been the subject of an article in print. In this paper, the author will strive to outline Dr. Mildred Cleveland's accomplishments, as well as to give the reader as clear a picture as possible of the woman who was the wife of one chiropractic pioneer and mother of another.

  3. Preeminent Biologist Joins CAS to Lead a New Research Center in Shanghai

    Institute of Scientific and Technical Information of China (English)

    2012-01-01

    Dr. ZHU Jiankang, an internationally renowned plant cell biologist and member of the US National Academy of Sciences, will work as Director of the Shanghai Center for Plant Stress Biology, which was officially inaugurated on April 28, 2012 in suburban Shanghai. Before he joined CAS through a key talent recruitment program sponsored by the Chinese government called the "Thousand Talents Program", Dr. ZHU had worked for many universities in the world and carried out leading innovative research on genetic and epigenetic mechanisms of plant responses to adverse environments such as salinity, drought and low temperature.

  4. CAS Scientist Receives Trieste Science Prize

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    @@ RAO Zihe, director of the CAS Institute of Biophysics and CAS Member, has been awarded the 2006Trieste Science Prize for "his worldclass contributions to structural biology and his studies of viruses responsible for human diseases."

  5. Dr Luigi Orlando, Dr Sergio Ceccuzzi, Dr. Armando Sbrana, Europa Metalli, Italy, Dr Albert Scherger, Member of KM Europa Metal AG, Osnabr ck, Germany, Prof. Filippo Menzinger, Scientific Attaché, Permanent Mission of Italy in Geneva

    CERN Multimedia

    Patrice Loïez

    2001-01-01

    Photo 01: Dr Lyn Evans and Dr Luigi Orlando Photo 04: L. to r.: Dr Lyn Evans, Dr Luigi Orlando, Prof. Luciano Maiani and Prof. Filippo Menzinger Photo 06: L. to r.: Prof. Philippo Menzinger, Dr Armando Sbrana, Prof. Luciano Maiani, Dr Albert Scherger, Dr Lyn Evans, Dr Luigi Orlando, Dr Sergio Ceccuzzi, visiting the LHC superconducting magnet test hall, SM18

  6. CAS to Address Water Eutrophication

    Institute of Scientific and Technical Information of China (English)

    Guo Haiyan; Zhao Baohua

    2002-01-01

    @@ At a meeting on April 5, the CAS authorities gave the green light to a proposal to carry out studies on the development and control of eutrophication on the middle and lower reaches of the Yangtze River. The five-year project,to cost 20 million yuan (about USS 2.4 million), will involve scientists from various CAS institutes, including the Nanjing Institute of Geography & Limnology, Wuhan Institute of Hydrobiology, Research Center for Eco-Environmental Sciences, Wuhan Institute of Botany, Institute of Chemistry, Institute of Geodesy & Geophysics, and Institute of Microbiology.

  7. CAS Academic Divisions in 2001

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    @@ 1.Election of New Members In 2001, 56 scientists were elected new CAS members, including l0 in the Division of Mathematics & Physics, 10 in the Division of Chemistry, 12 in the Division of Biology, nine in the Division of Earth Sciences and 15 in the Division of Engineering Sciences.The average age of the new members is 60.4, and the youngest one is 38 years old. They are now working in nine provinces or municipalities, or governmental departments under the State Council, including 23 outstanding experts working for the CAS.

  8. CAS Announces Academicians Elected in 2009

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    @@ Thirty five Chinese scientists and six of their colleagues from Canada,France and the United States were elected into CAS in 2009.The announcement was made by CAS President LU Yongxiang at a press conference held on December 4,2009,in Beijing.CAS Vice President LI Jinghai read the list of the new Members.

  9. CAS Headquarters Steps Up Efficiency Efforts

    Institute of Scientific and Technical Information of China (English)

    2004-01-01

    @@ As part of China's S&T system reform and national Knowledge Innovation Program (KIP) spearheaded by CAS, an effort to downsize the administration at the CAS headquarters has made significant progress,Vice President Yang Bailing announced at a conference for position-engagement at the CAS headquarters on Nov. 4 in Beijing.

  10. Diabetic retinopathy (DR: everybody's business

    Directory of Open Access Journals (Sweden)

    David Yorston

    2011-09-01

    Full Text Available Diabetes is on the increase worldwide, due mainly to the rise in the number of people with type 2 diabetes. Type 2 diabetes is becoming more common because:* People are living longer, and diabetes is more prevalent in older people.* As people increasingly migrate to urban areas, exercise less, eat more, and eat less healthy food, more people are becoming obese – a primary cause of type 2 diabetes.Diabetes increases the risk of a range of eye diseases, including cataract, but the main cause of blindness associated with diabetes is diabetic retinopathy (DR. DR usually develops between ten and twenty years after the onset of diabetes, and develops faster when diabetes is undiagnosed and untreated.People with DR whose sight is at risk can be treated, most commonly with laser, to prevent visual impairment and blindness. Sadly, there is no treatment that can restore vision that has already been lost.

  11. Tribute to Dr Jacques Rogge

    DEFF Research Database (Denmark)

    Bourgois, Jan G; Dumortier, Jasmien; Callewaert, Margot

    2017-01-01

    the former President of the International Olympic Committee, he was also an orthopaedic surgeon and a keen sailor, competing at three Olympic Games. In 1972, in fulfilment of the requirements for the degree of Master in Sports Medicine, he was the first who studied a sailors' muscle activity by means......'A tribute to Dr J. Rogge' aims to systematically review muscle activity and muscle fatigue during sustained submaximal quasi-isometric knee extension exercise (hiking) related to Olympic dinghy sailing as a tribute to Dr Rogge's merits in the world of sports. Dr Jacques Rogge is not only...... in Sports Medicine at the Ghent University. Hiking is a critical bilateral and multi-joint movement during dinghy racing, accounting for >60% of the total upwind leg time. Hiking generates large stresses in the anterior muscles that cross the knee and hip joint. Hiking is considered as a quasi...

  12. Profile Interview: Dr Desiree Witte

    African Journals Online (AJOL)

    Dr Albert Schweitzer, with stories about his hospital in. Lambarene, now ... active cases of vaccine-preventable diseases in children living ... aid program between the Dutch and Malawi Governments. I ... the delivery protected from the inquisitive eyes from people ... (LSHTM) and the World Health Organization (WHO).

  13. Dr. John Marburger visits DESY

    CERN Multimedia

    2003-01-01

    Dr. John Marburger, Director of the United States Office of Science and Technology Policy, visited the research center DESY in Hamburg. The American physicist wanted to inform himself about the status of the TESLA X-ray laser and the TESLA linear collider as well as the international collaboration at DESY (1/2 page).

  14. In Memoriam Dr. M. Jacobs

    NARCIS (Netherlands)

    Kalkman, C.

    1983-01-01

    Dr. Marius Jacobs, a senior staff member of the Rijksherbarium, died suddenly on 28 April 1983, following a heart attack some days earlier. He was only 53 years old and his death came as a great shock, not only to his colleagues at our institute. Jacobs was a many-sided man with interests in many fi

  15. In Memoriam Dr. M. Jacobs

    NARCIS (Netherlands)

    Kalkman, C.

    1983-01-01

    Dr. Marius Jacobs, a senior staff member of the Rijksherbarium, died suddenly on 28 April 1983, following a heart attack some days earlier. He was only 53 years old and his death came as a great shock, not only to his colleagues at our institute. Jacobs was a many-sided man with interests in many fi

  16. Dr. Hermann otto Sleumer retired

    NARCIS (Netherlands)

    Steenis, van C.G.G.J.

    1971-01-01

    On March 1st, 1971, Dr. Hermann Otto Sleumer, senior staff member of the Rijksherbarium, retired on reaching the age of 65 years. I have expressly omitted to say ‘from active service’, because his work has gone on uninterruptedly and he even had objections against spending one afternoon, on Febr. 26

  17. Salesian Psychological Center "Dr. P. Emilio Gambirasio". Career and Prospects

    Directory of Open Access Journals (Sweden)

    Eduardo Morán García

    2008-06-01

    Full Text Available El 11 de noviembre del 2007, luego de que el Centro Psicológico Salesiano (CPS cumpliera un aniversario más de trabajo, he considerado conveniente sistematizar las actividades realizadas,los logros alcanzados y sus proyecciones futuras en el momento de dejar su Dirección.

  18. Genome modification by CRISPR/Cas9.

    Science.gov (United States)

    Ma, Yuanwu; Zhang, Lianfeng; Huang, Xingxu

    2014-12-01

    Clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein (Cas)9-mediated genome modification enables us to edit the genomes of a variety of organisms rapidly and efficiently. The advantages of the CRISPR-Cas9 system have made it an increasingly popular genetic engineering tool for biological and therapeutic applications. Moreover, CRISPR-Cas9 has been employed to recruit functional domains that repress/activate gene expression or label specific genomic loci in living cells or organisms, in order to explore developmental mechanisms, gene expression regulation, and animal behavior. One major concern about this system is its specificity; although CRISPR-Cas9-mediated off-target mutation has been broadly studied, more efforts are required to further improve the specificity of CRISPR-Cas9. We will also discuss the potential applications of CRISPR-Cas9.

  19. Expanding CRISPR/Cas9 Genome Editing Capacity in Zebrafish Using SaCas9

    Directory of Open Access Journals (Sweden)

    Yan Feng

    2016-08-01

    Full Text Available The type II CRISPR/Cas9 system has been used widely for genome editing in zebrafish. However, the requirement for the 5′-NGG-3′ protospacer-adjacent motif (PAM of Cas9 from Streptococcus pyogenes (SpCas9 limits its targeting sequences. Here, we report that a Cas9 ortholog from Staphylococcus aureus (SaCas9, and its KKH variant, successfully induced targeted mutagenesis with high frequency in zebrafish. Confirming previous findings, the SpCas9 variant, VQR, can also induce targeted mutations in zebrafish. Bioinformatics analysis of these new Cas targets suggests that the number of available target sites in the zebrafish genome can be greatly expanded. Collectively, the expanded target repertoire of Cas9 in zebrafish should further facilitate the utility of this organism for genetic studies of vertebrate biology.

  20. Sproglige drømmerier

    DEFF Research Database (Denmark)

    Farø, Ken Joensen

    2015-01-01

    blev tidligere brugt i Danmarks Radio som pausesignal, afspillet på en spilledåse. Ak ja, det var dengang. Gå ind på nettet og lyt til den, hvis du ikke kender melodien. Det er national kulturarv. Mange bevingede ord indeholder en form af ”drøm(me)”, fx Martin Luther Kings ”I have a Dream”. Eller...

  1. Kubrick's Dr. Strangelove: A Guide to Study

    Science.gov (United States)

    Carringer, Robert

    1974-01-01

    Article provided an analysis of the films of Stanley Kubrick, credits for the film, Dr. Strangelove, a sequence outline of Dr. Strangelove and study questions relating to the film. It further made suggestions for additional reading. (RK)

  2. Obituary: Dr Dianne Johnson (1947-2012)

    CERN Document Server

    Hamacher, Duane W

    2014-01-01

    It is with great sadness that I report the passing of Dr Dianne Johnson in May 2012. Dr Johnson was a pivotal and important figure in the field of Australian cultural astronomy and in the campaign for Aboriginal rights.

  3. Optimizing Decision Tree Attack on CAS Scheme

    Directory of Open Access Journals (Sweden)

    PERKOVIC, T.

    2016-05-01

    Full Text Available In this paper we show a successful side-channel timing attack on a well-known high-complexity cognitive authentication (CAS scheme. We exploit the weakness of CAS scheme that comes from the asymmetry of the virtual interface and graphical layout which results in nonuniform human behavior during the login procedure, leading to detectable variations in user's response times. We optimized a well-known probabilistic decision tree attack on CAS scheme by introducing this timing information into the attack. We show that the developed classifier could be used to significantly reduce the number of login sessions required to break the CAS scheme.

  4. White Dwarfs in SDSS DR9 and DR10

    Science.gov (United States)

    Gentile Fusillo, Nicola Pietro; Gänsicke, Boris; Koester, Detlev

    2015-06-01

    Currently the largest catalogue of spectroscopically identified WDs is based on the 7th Data Release (DR) of the Sloan Digital Sky Survey and contains over 20000 WDs (Kleinman et al. 2013). However, only a fraction of all WDs in the photometric footprint of SDSS have been spectroscopically followed up. Using DR7 spectroscopy as a training sample, we developed a method to select high confidence photometric WD candidates. The novelty of our selection is that it allows us to assign to any object with multi-colour and proper motion data a well-defined "probability of being a white dwarf" (or a contaminant). Exploiting this selection method we compiled a catalogue (Gentile Fusillo et al. in prep) which currently covers the entire photometric footprint of SDSS, 14555sq deg, with a limiting magnitude of g ≤ 19. The catalogue contains over 20000 high-confidence WDs and WD candidates 11500 of which have not yet been followed up with Sloan spectroscopy. Even though, so far, our catalogue relies only SDSS we plan to extend the sky coverage as additional deep multi-colour large area surveys become available. DR10 includes over 1.4 million spectra taken with the new BOSS spectrograph, which improves over the original SDSS spectograph in both resolution and wavelength coverage, but has so far not been systematically mined for WD science. As part of this project, we also inspected over 8000 BOSS spectra of bright (g ≤ 19) colour selected sources and classified 1765 new WDs. We used this independent, spectroscopically confirmed sample to further validate our selection method. Finally we discuss possible application of our catalogue , focusing on the selection and follow up of 9 new DZs which show strong pollution from elements other than Ca and IR excess emission emission consistent with the presence of debris discs.

  5. Dr Jan Theodoor Henrard (with portrait)

    NARCIS (Netherlands)

    Goddijn, W.A.

    1946-01-01

    On October 16th 1946 Dr J. Th. Henrard will have reached the pensionable age of sixty five years. In accordance with the legal prescriptions he is due to take leave officially as keeper of the ”Rijksherbarium“. The present director, Prof. Dr H. J. Lam, invited me to write a short biography of Dr

  6. Dr Jan Theodoor Henrard (with portrait)

    NARCIS (Netherlands)

    Goddijn, W.A.

    1946-01-01

    On October 16th 1946 Dr J. Th. Henrard will have reached the pensionable age of sixty five years. In accordance with the legal prescriptions he is due to take leave officially as keeper of the ”Rijksherbarium“. The present director, Prof. Dr H. J. Lam, invited me to write a short biography of Dr Hen

  7. FOREWORD: Dr Trevor J Hicks Dr Trevor J Hicks

    Science.gov (United States)

    Goossens, Darren

    2009-03-01

    This issue of Journal of Physics: Condensed Matter has been assembled to recognize the valuable contribution of Dr Trevor J Hicks to the field of neutron scattering and magnetism. Trevor began his study of magnetism as a PhD student at Monash University in Melbourne in the early 1960s, working with Professor Jack Smith. From the very beginning magnetism in alloys, and disordered systems in general, became a key aspect of his career. After a postdoctoral position at Harwell working with Dr Graeme Low Trevor returned to Australia and took up a position with Monash. He soon became a key figure in developing the capability for neutron scattering using the HIFAR reactor at the Australian Atomic Energy Commission, now the Australian Nuclear Science and Technology Organisation, ANSTO. The instrumentation was always developed to further his studies of magnetism. The development of polarization analysis measurements of diffuse magnetic scattering, first using iron filters and then his own design of supermirror benders for beam polarization, took place through the 1970s, 1980s and into the 1990s. Throughout this time, Trevor mentored a series of PhD students and postdoctoral fellows, many of whom have contributed to this issue (and, indeed, guest edited it). As befits a scientist and university academic for whom teaching has always been important, Trevor has not only created a strong body of significant research, he has also made a major contribution to preparing several generations of neutron scattering scientists, and this issue reflects that. When I approached Journal of Physics: Condensed Matter with a proposal for an issue in honour of Trevor, the response was immediate and positive. It is with great pleasure that I present the result of that proposal. The great diversity of the content, all centred on neutron scattering and magnetism, reflects the breadth of Trevor's own career and of the scientists with whom he has interacted. Finally, I would like to make some

  8. Interview with Dr Anna Matamala

    Directory of Open Access Journals (Sweden)

    Lucinea Marcelino Villela

    2016-12-01

    Full Text Available In this interview, which took place in June 2016, Dr Anna Matamala described some details about her long professional experience in Audiovisual Translation, especially in dubbing from English into Catalan, and we talked about many other things like her interest in lexicography, her point of view on some contemporary topics in Audiovisual Translation Studies: the use of technology, the relation between AVT and Accessibility Studies, AVT and Filmmaking fields, the importance of keeping in touch with other countries and even continents outside Europe, and she also gave some advice to the new generation of Translation students.

  9. Semiotic and discursive variables in cas-based didactical engineering

    DEFF Research Database (Denmark)

    Winsløw, Carl

    2003-01-01

    CAS, didactical engeneering, Maple, semiotics, undergraduate teaching, mathematics, education, didactics......CAS, didactical engeneering, Maple, semiotics, undergraduate teaching, mathematics, education, didactics...

  10. Structural characterization of CAS SH3 domain selectivity and regulation reveals new CAS interaction partners.

    Science.gov (United States)

    Gemperle, Jakub; Hexnerová, Rozálie; Lepšík, Martin; Tesina, Petr; Dibus, Michal; Novotný, Marian; Brábek, Jan; Veverka, Václav; Rosel, Daniel

    2017-08-14

    CAS is a docking protein downstream of the proto-oncogene Src with a role in invasion and metastasis of cancer cells. The CAS SH3 domain is indispensable for CAS-mediated signaling, but structural aspects of CAS SH3 ligand binding and regulation are not well understood. Here, we identified the consensus CAS SH3 binding motif and structurally characterized the CAS SH3 domain in complex with ligand. We revealed the requirement for an uncommon centrally localized lysine residue at position +2 of CAS SH3 ligands and two rather dissimilar optional anchoring residues, leucine and arginine, at position +5. We further expanded the knowledge of CAS SH3 ligand binding regulation by manipulating tyrosine 12 phosphorylation and confirmed the negative role of this phosphorylation on CAS SH3 ligand binding. Finally, by exploiting the newly identified binding requirements of the CAS SH3 domain, we predicted and experimentally verified two novel CAS SH3 binding partners, DOK7 and GLIS2.

  11. 51 CAS Members Elected in 2005

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    @@ Atotal of 51 prominent Chinese scientists were elected CAS members in 2005. The announce ment was made at a press conference held on Dec. 16 in Beijing. CAS President LU Yongxiang was present at the conference to give a briefing on the election and the new members.

  12. International Cooperation at CAS Since 1998

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    @@ Since 1998 when the Knowledge Innovation Program (KIP) was launched,the rapid development of CAS has offered great opportunities to forge international cooperation.Focusing on capacity building to effectively absorb global innovation resources and through organizing major innovative activities,CAS has increasingly expanded and intensified international partnerships.

  13. CAS paleoichthyologist gives Artedi Lecture in Sweden

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    @@ Prof. ZHANG Miman (CHANG Mee-mann), a CAS Member from the CAS Institute of Vertebrate Paleontology and Paleoanthropology, was invited to give a talk at the Artedi Lectures at the Royal Swedish Academy of Sciences in Stockholm, Sweden, on 5 December, 2008.

  14. CAS, Max Planck Society to Enhance Cooperation

    Institute of Scientific and Technical Information of China (English)

    2004-01-01

    @@ According to a briefing issued by the CAS Bureau of In ternational Cooperation on May 8, 2004, CAS and the Max Planck Society (MPS) in Germany are considering to establish a multidisciplinary institute in Shanghai to conduct research into computational biology. The move is applauded as a fresh step in promoting Sino-German S&T cooperation.

  15. CAS, Sinopec forge all-round partnership

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    @@ CAS and China Petroleum & Chemical Corporation (Sinopec), the 3nd largest oil refiner in the world, inked an agreement for comprehensive strategic cooperation on 23 January in Beijing. Sinopec President WANG Tianpu and CAS Vice President SHI Erwei were present to witness the event

  16. CAS Joins Asian Consortium of Science Academies

    Institute of Scientific and Technical Information of China (English)

    2004-01-01

    @@ CAS formally became a member of the Association of Academies of Sciences in Asia (AASA) at the fourth general assembly of AASA from Sept. 23 to 25 in Tehran, Iran. A CAS delegation headed by Vice-President Yang Bailing attended the conference.

  17. CAS issues its 2008 Science & Society Series

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    @@ CAS recently released its 2008 Science and Society Series consisting of three reports on scientific progress, high-tech advancement and sustainable development. Among them, the latter two were completed under the organization of the CAS Institute of Policy and Management.

  18. CAS Launches Website for Scientific Education

    Institute of Scientific and Technical Information of China (English)

    2004-01-01

    @@ CAS member Wang Shouguan and SecretaryGeneral of the Ministry of Science and Technology Zhang Jing'an jointly push the button on August 26 in Beijing to launch a CAS website for scientific education (http ://www.fipse. cn/).

  19. CasA mediates Cas3-catalyzed target degradation during CRISPR RNA-guided interference.

    Science.gov (United States)

    Hochstrasser, Megan L; Taylor, David W; Bhat, Prashant; Guegler, Chantal K; Sternberg, Samuel H; Nogales, Eva; Doudna, Jennifer A

    2014-05-06

    In bacteria, the clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) DNA-targeting complex Cascade (CRISPR-associated complex for antiviral defense) uses CRISPR RNA (crRNA) guides to bind complementary DNA targets at sites adjacent to a trinucleotide signature sequence called the protospacer adjacent motif (PAM). The Cascade complex then recruits Cas3, a nuclease-helicase that catalyzes unwinding and cleavage of foreign double-stranded DNA (dsDNA) bearing a sequence matching that of the crRNA. Cascade comprises the CasA-E proteins and one crRNA, forming a structure that binds and unwinds dsDNA to form an R loop in which the target strand of the DNA base pairs with the 32-nt RNA guide sequence. Single-particle electron microscopy reconstructions of dsDNA-bound Cascade with and without Cas3 reveal that Cascade positions the PAM-proximal end of the DNA duplex at the CasA subunit and near the site of Cas3 association. The finding that the DNA target and Cas3 colocalize with CasA implicates this subunit in a key target-validation step during DNA interference. We show biochemically that base pairing of the PAM region is unnecessary for target binding but critical for Cas3-mediated degradation. In addition, the L1 loop of CasA, previously implicated in PAM recognition, is essential for Cas3 activation following target binding by Cascade. Together, these data show that the CasA subunit of Cascade functions as an essential partner of Cas3 by recognizing DNA target sites and positioning Cas3 adjacent to the PAM to ensure cleavage.

  20. In vitro enzymology of Cas9.

    Science.gov (United States)

    Anders, Carolin; Jinek, Martin

    2014-01-01

    Cas9 is a bacterial RNA-guided endonuclease that uses base pairing to recognize and cleave target DNAs with complementarity to the guide RNA. The programmable sequence specificity of Cas9 has been harnessed for genome editing and gene expression control in many organisms. Here, we describe protocols for the heterologous expression and purification of recombinant Cas9 protein and for in vitro transcription of guide RNAs. We describe in vitro reconstitution of the Cas9-guide RNA ribonucleoprotein complex and its use in endonuclease activity assays. The methods outlined here enable mechanistic characterization of the RNA-guided DNA cleavage activity of Cas9 and may assist in further development of the enzyme for genetic engineering applications.

  1. Dr. Chang Min Chueh——Father of Test Tube Babies

    Institute of Scientific and Technical Information of China (English)

    1994-01-01

    CHANG Min Chueh (1908-1991)—the father of test tube babies, was a Chinese-born American scientist and inventor who developed the first oral contraceptives. Dr. Chang was from Dunhou Township, Lanxian County, Shanxi Province. In middle school, he was fascinated with biology. Later he was accepted to the Animal Psychology Department at Qinghua University.

  2. PCCF flow analysis -- DR Reactor

    Energy Technology Data Exchange (ETDEWEB)

    Calkin, J.F.

    1961-04-26

    This report contains an analysis of PCCF tube flow and Panellit pressure relations at DR reactor. Supply curves are presented at front header pressures from 480 to 600 psig using cold water and the standard 0.236 inch orifice with taper down stream and the pigtail valve (plug or ball) open. Demand curves are presented for slug column lengths of 200 inches to 400 inches using 1.44 inch O.D. solid poison pieces (either Al or Pb-Cd) and cold water with a rear header pressure of 50 psig. Figure 1 is a graph of Panellit pressure vs. flow with the above supply and demand curves and clearly shows the effect of front header pressure and charge length on flow.

  3. Dr House, TV, and reality...

    Science.gov (United States)

    Lapostolle, Frédéric; Montois, Sylvie; Alhéritière, Armelle; De Stefano, Carla; Le Toumelin, Philippe; Adnet, Frédéric

    2013-02-01

    Medical practice in the media is usually far from reality. Thus, the viewer may be led astray. The world-famous fictional Dr House has to face a difficult diagnosis every week. His practice does not seem to reflect reality. The aim of this study was to assess the diagnosis strategy involved in this television program. An observer has previewed the 2011 season. The episode running time, the patient's age and sex, the list of all investigations and interventions, the final diagnosis, and the patient's outcome were collected. Number and proportion of French viewers for each episode were recorded. We analyzed 18 episodes. The median running time was 42.5 (42.1-43.2) minutes. Main patient characters were 12 men (66%) and 6 women (33%); the average age was 31 (22-38) years. There were 225 investigations or interventions reported, averaging 14 (9-15) per episode, representing one examination every 3.1 (2.9-4.8) minutes. The most frequently prescribed investigations were magnetic resonance imaging (MRI; 13; 72%), blood sample (11; 61%), and biopsy (10; 56%). The most frequent interventions were surgery, anti-infectious treatments, and steroid treatments (9 each; 50%). Two patients (11%) died. The median number of spectators was 8.4 (8.1-8.7) million, corresponding to 33% (33%-34%) of the French national audience. The population and the examination strategies used by Dr House were unrealistic. Because of this distortion, patients may not understand, nor accept the delay, the investigation choices, the intervention costs, risks, nor failures of a daily medical practice. Physicians should be aware of this "information bias." Copyright © 2013 Elsevier Inc. All rights reserved.

  4. CAS at the Cockcroft Institute

    CERN Multimedia

    2007-01-01

    The CERN Accelerator School (CAS) and the UK’s new centre for accelerator science, the Cockcroft Institute, jointly organised a course on General Accelerator Physics, at intermediate level, at the Cockcroft Institute, Daresbury, UK, from 16 to 28 September 2007. The venue took advantage of the excellent new facilities in the Institute and the existing infrastructure of the adjacent Daresbury Laboratory. The intermediate level course followed established practice, with lectures on core topics in the mornings and specialised courses in the afternoons. The latter provided "hands-on" education and experience in the three selected topics: "RF Measurements Techniques", "Beam Instrumentation and Diagnostics" and "Optics Design and Correction". These proved to be highly successful, with participants choosing one course and following the topic throughout the school. Guided studies, tutorials, seminars and a poster session completed the prog...

  5. Factor Affecting the Computerised Accounting System (CAS) Usage in Public Sector

    OpenAIRE

    AZLEEN ILIAS; NURUL NAZIRAH BINTI ZAINUDIN

    2013-01-01

    The purpose is to examine the significant factors of Technology Acceptance Model (TAM) (perceived ease of use, perceived usefulness, behavioural intention, actual use, and attitude toward using) and Psychological Attachment (compliance, identification as well as internalization) towards Computerised Accounting System (CAS) in Malaysian Accountant General Department (MAGD). This research is based on 99 valid respondents collected from a survey questionnaire and multiple regressions were employ...

  6. Dr. von Braun Briefing Walt Disney

    Science.gov (United States)

    1965-01-01

    Dr. von Braun began his association with Walt Disney in the 1950s when the rocket scientist appeared in three Disney television productions related to the exploration of space. Years later, Dr. von Braun invited Disney and his associates to tour the Marshall Space Flight Center (MSFC) in Huntsville, Alabama. This photograph is dated April 13, 1965. From left are R.J. Schwinghamer from the MSFC, Disney, B.J. Bernight, and Dr. von Braun.

  7. In Memoriam: Dr. Frank John Fenner

    Centers for Disease Control (CDC) Podcasts

    2011-04-22

    This podcast reflects on one of the greatest pioneers in virology, Dr. Frank John Fenner. Dr. Frederick Murphy, a member of EID's editorial board and the Institute of Medicine, and professor of Pathology at the University of Texas Medical Branch in Galveston, shares professional and personal stories of Dr. Frank Fenner.  Created: 4/22/2011 by National Center for Emerging Zoonotic and Infectious Diseases (NCEZID).   Date Released: 4/26/2011.

  8. Assisting Students' Cognitive Strategies with the Use of CAS

    Science.gov (United States)

    Sarvari, Csaba; Lavicza, Zsolt; Klincsik, Mihaly

    2010-01-01

    This paper examines various cognitive strategies applied while CAS (Computer Algebra System) are used in undergraduate-level engineering mathematics teaching and learning. We posed some questions in relation to such CAS use: What kind of tools can CAS offer to enhance different cognitive strategies of students? How can the use of CAS widen the…

  9. Homenaje al Dr. Eduardo Vargas Alvarado

    OpenAIRE

    Carlos Abarca Barrantes; Leslie Solano Calderón

    2003-01-01

    La Universidad Latina rinde homenaje al Dr. Eduardo Vargas Alvarado, Decano de la Facultad de Medicina de esta Universidad. El Dr. Eduardo VaIgas Alvarado desarrollo la Morgue Judicial, desde su inicio en de enero de 1965, y desde 1975 hasta su jubilación del Poder Judicial en 1992 fue el Jefe del Departamento de Medicina Legal. La Dra. Leslie Solano Calderón es la Jefa actual de dicho Departamento y junto con el Dr. Abarca, fueron discípulos de Don Eduardo. El Dr. Vargas ha escrito varios li...

  10. China's Investment Leade Dr, Alyce Su

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    @@ I. Professional Background Dr. Alyce Su specializes in investment managemeng, managing portfolios consisted of investment opportunities originated from China's growth and internationalization, both'outbound and inbound.

  11. Nucleosomes Inhibit Cas9 Endonuclease Activity in Vitro.

    Science.gov (United States)

    Hinz, John M; Laughery, Marian F; Wyrick, John J

    2015-12-01

    During Cas9 genome editing in eukaryotic cells, the bacterial Cas9 enzyme cleaves DNA targets within chromatin. To understand how chromatin affects Cas9 targeting, we characterized Cas9 activity on nucleosome substrates in vitro. We find that Cas9 endonuclease activity is strongly inhibited when its target site is located within the nucleosome core. In contrast, the nucleosome structure does not affect Cas9 activity at a target site within the adjacent linker DNA. Analysis of target sites that partially overlap with the nucleosome edge indicates that the accessibility of the protospacer-adjacent motif (PAM) is the critical determinant of Cas9 activity on a nucleosome.

  12. A newly discovered Bordetella species carries a transcriptionally active CRISPR-Cas with a small Cas9 endonuclease

    Science.gov (United States)

    The Cas9 endonuclease of the Type II-a clustered regularly interspersed short palindromic repeats (CRISPR), of Streptococcus pyogenes (SpCas9) has been adapted as a widely used tool for genome editing and genome engineering. Herein, we describe a gene encoding a novel Cas9 ortholog (BpsuCas9) and th...

  13. Cas9 specifies functional viral targets during CRISPR-Cas adaptation.

    Science.gov (United States)

    Heler, Robert; Samai, Poulami; Modell, Joshua W; Weiner, Catherine; Goldberg, Gregory W; Bikard, David; Marraffini, Luciano A

    2015-03-12

    Clustered regularly interspaced short palindromic repeat (CRISPR) loci and their associated (Cas) proteins provide adaptive immunity against viral infection in prokaryotes. Upon infection, short phage sequences known as spacers integrate between CRISPR repeats and are transcribed into small RNA molecules that guide the Cas9 nuclease to the viral targets (protospacers). Streptococcus pyogenes Cas9 cleavage of the viral genome requires the presence of a 5'-NGG-3' protospacer adjacent motif (PAM) sequence immediately downstream of the viral target. It is not known whether and how viral sequences flanked by the correct PAM are chosen as new spacers. Here we show that Cas9 selects functional spacers by recognizing their PAM during spacer acquisition. The replacement of cas9 with alleles that lack the PAM recognition motif or recognize an NGGNG PAM eliminated or changed PAM specificity during spacer acquisition, respectively. Cas9 associates with other proteins of the acquisition machinery (Cas1, Cas2 and Csn2), presumably to provide PAM-specificity to this process. These results establish a new function for Cas9 in the genesis of prokaryotic immunological memory.

  14. CAS Readjusts Implementation of Its Talent Program

    Institute of Scientific and Technical Information of China (English)

    Guo Haiyan

    2002-01-01

    @@ CAS has decided to readjust the way of implementing its Century Program (or Hundred-Talents Program), to give more independence to research institutes in head hunting and guarantee the support for the Program recruits on a selective basis.

  15. AKRO/SF: Catch Accounting System (CAS)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Catch Accounting System (CAS) creates total catch estimates for the groundfish fisheries in the Bering Sea/Aleutian Islands and Gulf of Alaska. Each year, quotas...

  16. CAS Scientists Develop China's First RFQ Accelerator

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    @@ With the support of the National Program on Key Basic Research Projects (dubbed the "973Program"), researchers from the CAS Institute of High Energy Physics (IHEP) have developed the first high-power Radio Frequency Quadrupole (RFQ) accelerator in China.

  17. The new CAS-DIS digital ionosonde

    Directory of Open Access Journals (Sweden)

    Wang Shun

    2013-04-01

    Full Text Available A high quality digital ionosonde called the Chinese Academy of Sciences digital ionosonde (CAS-DIS has been developed for investigations of the ionosphere. Two important features are used for the CAS-DIS; first, the technique of analog down-conversion has been replaced by the new approach of digital down-conversion technology. Secondly, to solve the problem of large instantaneous receiving bandwidth in digital receivers, an analog narrowband tracking filter is used for the CAS-DIS. The center frequency of the filter tracks the carrier frequency transmitted in real-time, to ensure that the frequency components are filtered out of the effective bandwidth. This report describes the system architecture of the CAS-DIS, its main features, and its test results for ionosphere detection. 

  18. Heaviest yet LBO monocrystal grown at CAS

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    @@ After three years of hard work, a research team led by Prof. HU Zhanggui with the CAS Technical Institute of Physics and Chemistry has made breakthrough progress in the studies of lithium triborate (LBO), a nonlinear optical crystal.

  19. Dr Andrea Granelli, Vice President, Telecom Italia

    CERN Multimedia

    Patrice Loïez

    2002-01-01

    Photo 06: Dr Andrea Granelli, Chief Executive Officer, Telecom Italia Lab (second from right) visiting the LHC superconducting magnet test hall with (from left to right) M. Cecchi , F. Gagliardi and G. Cavallari. Photo 15: Dr Andrea Granelli, Chief Executive Officer, Telecom Italia Lab (left) visiting the LHC superconducting magnet test hall with (from left to right) M. Cecchi and G. Cavallari.

  20. Biography of Dr. Simao Nascimento de Sousa

    Digital Repository Service at National Institute of Oceanography (India)

    Sarkar, A.^red.

    the projects he has handled are the Coastal Ocean Monitoring and Prediction System (COMAPS) and Integrated Coastal and Marine Area Management Plan for Goa (ICMAM-Goa). Dr. de Sousa was a silent worker of high caliber with exceptional leadership qualities. Dr. A...

  1. Dr Valter Rukavina - amateur painter.

    Science.gov (United States)

    Glavocic, Daina

    2008-01-01

    In this essay Dr Valter Rukavina (Rijeka 1896-1972), excellent specialist in infectious diseases and professor of the Rijeka University School of Medicine, is presented as successful amateur painter. He had been refining his talent through relentless practice since the school days, complementing it with skills and advice from established painters he associated with. He favoured figurative, realistic and somewhat romantic expression for his themes such as coastal landscapes, marinas, Quarnero sceneries, still life in tempera or oil, and drawings in ink or sepia. Despite partial colour blindness, he successfully used colour. He featured in a number of group exhibitions such as that of amateur painters of Rijeka in 1950, of painters physicians of Yugoslavia (Zagreb, 1956), in the Second International Exhibition of Contemporary Art (Florence, 1964), exhibition of the Rijeka branch of the Croatian Association of Visual Artists (Belgrade, 1966), and the 1969 exhibition in Opatija. His native city hosted two one-man exhibitions, the first retrospective in 1971, while he was still alive, and the second posthumous in 2007, with a good selection of his life's work.

  2. CRISPR-Cas: biology, mechanisms and relevance.

    Science.gov (United States)

    Hille, Frank; Charpentier, Emmanuelle

    2016-11-01

    Prokaryotes have evolved several defence mechanisms to protect themselves from viral predators. Clustered regularly interspaced short palindromic repeats (CRISPR) and their associated proteins (Cas) display a prokaryotic adaptive immune system that memorizes previous infections by integrating short sequences of invading genomes-termed spacers-into the CRISPR locus. The spacers interspaced with repeats are expressed as small guide CRISPR RNAs (crRNAs) that are employed by Cas proteins to target invaders sequence-specifically upon a reoccurring infection. The ability of the minimal CRISPR-Cas9 system to target DNA sequences using programmable RNAs has opened new avenues in genome editing in a broad range of cells and organisms with high potential in therapeutical applications. While numerous scientific studies have shed light on the biochemical processes behind CRISPR-Cas systems, several aspects of the immunity steps, however, still lack sufficient understanding. This review summarizes major discoveries in the CRISPR-Cas field, discusses the role of CRISPR-Cas in prokaryotic immunity and other physiological properties, and describes applications of the system as a DNA editing technology and antimicrobial agent.This article is part of the themed issue 'The new bacteriology'.

  3. CRISPR-Cas: biology, mechanisms and relevance

    Science.gov (United States)

    Hille, Frank

    2016-01-01

    Prokaryotes have evolved several defence mechanisms to protect themselves from viral predators. Clustered regularly interspaced short palindromic repeats (CRISPR) and their associated proteins (Cas) display a prokaryotic adaptive immune system that memorizes previous infections by integrating short sequences of invading genomes—termed spacers—into the CRISPR locus. The spacers interspaced with repeats are expressed as small guide CRISPR RNAs (crRNAs) that are employed by Cas proteins to target invaders sequence-specifically upon a reoccurring infection. The ability of the minimal CRISPR-Cas9 system to target DNA sequences using programmable RNAs has opened new avenues in genome editing in a broad range of cells and organisms with high potential in therapeutical applications. While numerous scientific studies have shed light on the biochemical processes behind CRISPR-Cas systems, several aspects of the immunity steps, however, still lack sufficient understanding. This review summarizes major discoveries in the CRISPR-Cas field, discusses the role of CRISPR-Cas in prokaryotic immunity and other physiological properties, and describes applications of the system as a DNA editing technology and antimicrobial agent. This article is part of the themed issue ‘The new bacteriology’. PMID:27672148

  4. CRISPR-Cas9 Structures and Mechanisms.

    Science.gov (United States)

    Jiang, Fuguo; Doudna, Jennifer A

    2017-05-22

    Many bacterial clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated (Cas) systems employ the dual RNA-guided DNA endonuclease Cas9 to defend against invading phages and conjugative plasmids by introducing site-specific double-stranded breaks in target DNA. Target recognition strictly requires the presence of a short protospacer adjacent motif (PAM) flanking the target site, and subsequent R-loop formation and strand scission are driven by complementary base pairing between the guide RNA and target DNA, Cas9-DNA interactions, and associated conformational changes. The use of CRISPR-Cas9 as an RNA-programmable DNA targeting and editing platform is simplified by a synthetic single-guide RNA (sgRNA) mimicking the natural dual trans-activating CRISPR RNA (tracrRNA)-CRISPR RNA (crRNA) structure. This review aims to provide an in-depth mechanistic and structural understanding of Cas9-mediated RNA-guided DNA targeting and cleavage. Molecular insights from biochemical and structural studies provide a framework for rational engineering aimed at altering catalytic function, guide RNA specificity, and PAM requirements and reducing off-target activity for the development of Cas9-based therapies against genetic diseases.

  5. Monocytic HLA DR antigens in schizophrenic patients.

    Science.gov (United States)

    Krause, Daniela; Wagner, Jenny; Matz, Judith; Weidinger, Elif; Obermeier, Michael; Riedel, Michael; Gruber, Rudolf; Schwarz, Markus; Mueller, Norbert

    2012-01-01

    A genetic association of specific human leukocyte antigens (HLA) DR genes and schizophrenia has recently been shown. These HLA play a fundamental role in the control of immune responses. Furthermore infectious agents have been proposed to be involved in the pathogenesis of schizophrenia. In this study we investigated the rate of HLA DR positive monocytes in schizophrenic patients compared to controls with a special focus on the adaption to in vitro stimulation with toll-like receptor ligands. Patients with schizophrenia and matched controls were included. For each individual, we evaluated the rate of HLA DR positive monocytes (either incubated at 37 °C or after stimulation with lipopolysaccharide or Poly I:C). We found a significantly higher percentage of schizophrenic patients with elevated HLA DR positive cells (p=0.045) as compared to controls. The adjustment rate from baseline levels of monocytic HLA DR positive cells to stimulation with Poly I:C was significantly lower in schizophrenic patients (p=0.038). The increased monocytic HLA DR in schizophrenic patients and the maladjustment of their monocytic HLA DR levels to an infectious stimulus might be a sign for a disturbed monocytic immune balance in schizophrenic individuals.

  6. Homenaje al Dr. Eduardo Vargas Alvarado

    Directory of Open Access Journals (Sweden)

    Carlos Abarca Barrantes

    2003-03-01

    Full Text Available La Universidad Latina rinde homenaje al Dr. Eduardo Vargas Alvarado, Decano de la Facultad de Medicina de esta Universidad. El Dr. Eduardo VaIgas Alvarado desarrollo la Morgue Judicial, desde su inicio en de enero de 1965, y desde 1975 hasta su jubilación del Poder Judicial en 1992 fue el Jefe del Departamento de Medicina Legal. La Dra. Leslie Solano Calderón es la Jefa actual de dicho Departamento y junto con el Dr. Abarca, fueron discípulos de Don Eduardo. El Dr. Vargas ha escrito varios libros de Medicina Legal, ha recibido múltiples reconocimientos por su labor en la Medicina Legal Latinoamericana y es el Presidente Honorario de la Asociación Costarricense de Medicina Forense. El Departamento de Medicina Legal y la Asociación Costarricense de Medicina Forense se han sumado a este justo y oportuno reconocimiento.The Latina University give honor to Dr. Eduardo Vargas Alvarado, Dean of the Medicine Faculty of this University. Dr. Vargas developed the Judicial Morgue, from its very beginning, January 1, of 1965; and from 1975 until his jubilation from the Judicial Power in 1992 was the Boss of the Legal Medicine Department. Dr. Leslie Solano Calderon, the actual Boss of this Department, and Dr. Abarca were disciples of Mr. Vargas. Dr. Vargas has written many books of Legal Medicine and has received many acknowledgements because of his legal medicine work in Latin America and is the Honorary President of the Costa Rican Forensic Medicine Association. The Legal Medicine Department and the Costa Rican Forensic Medicine Association are added to this just and opportune acknowledge.

  7. An Interview with Dr. Walter Lear

    Directory of Open Access Journals (Sweden)

    The Editors

    2009-03-01

    Full Text Available In this issue of the English version of Social Medicine we are publishing the first of several pamphlets loaned to us by the US Health Activism History Collection. To introduce this collection we travelled to Philadelphia on June 18, 2008 to interview Dr. Walter J. Lear. Dr Lear, born in 1923, is the person responsible for the collection. In a wide-ranging interview in his home Dr. Lear discussed his personal background, the origins and purpose of the collection, the impact of the McCarthy period on the US health left, as well as his vision for the future.

  8. Analysis of Coupling between DR and Microstrip

    Institute of Scientific and Technical Information of China (English)

    LIAO Juan; LIU Guanghu

    2003-01-01

    The coupling between dielectric resonator (DR) and microstrip is theoretically analyzed in this paper. The equivalent circuit and corresponding components' expression are achieved. Also, the formula of the coupling ratio is deduced, which is the critical parameter to calculate the values of components. The analysis of the paper can be used in modeling and strict design in DR applications such as dielectric resonator oscillators(DROs)and DR filters. At the end of the paper, an example of DRO design based on the analysis above is given. The coincidence between modulation and measure shows the significance of the paper.

  9. Obituary - Dr. A. Venkoba Rao

    Directory of Open Access Journals (Sweden)

    Ajai R. Singh

    2005-11-01

    Full Text Available I have to inform with great regret the sad demise of Dr Venkoba Rao, one of the senior most and highly esteemed psychiatrists this country has produced. His research work in psychiatry and medicine, as well as his work in philosophy and the human predicament, have been noteworthy indeed. He was a researcher and an academician of the highest order.It was always a great pleasure interacting with him. Both juniors and his peers will remember him with great regard and equal fondness. He never made himself difficult to approach, and was open to all positive inputs, from whatever source.He was highly appreciative of the work done by the Mens Sana Research Foundation too. I treasure the encouraging letter he wrote to us sometime back about the Mens Sana Monograph Psychiatry, Science, Religion and Health, of which here is an excerpt:The articles in general are of high standard and are very readable.....Imust tell you that your monograph makes an enjoyable and informative reading and my personal congratulations to you on your achievement.In fact, he was encouraging towards all efforts in the field of mental health.His wide knowledge of philosophy and Indian thought, coupled with active research interest in mainstream psychiatry, made him a unique presence in the field.His absence will be felt for a long long time indeed.But I have lived, and have not lived in vain;My mind may lose its force, my blood its fire,And my frame perish even in conquering pain:But there is that within me which shall tireTorture and Time, and breathe when I expire;Something unearthly, which they deem not of,Like the remembered tone of a mute lyre....Byron ( Childe Harold On behalf of the Mens Sana Editorial Board, our subscribers, readers, as well as the Mensanamonographs group, I offer our deepest condolences to his bereaved family.May his soul rest in peace.

  10. Award for Distinguished Contributions to the International Advancement of Psychology.

    Science.gov (United States)

    2016-11-01

    This award is given to individuals who have made sustained and enduring contributions to international cooperation and the advancement of knowledge in psychology. The 2016 recipient of the APA Award for Distinguished Contributions to the International Advancement of Psychology was selected by the 2015 Committee on International Relations in Psychology (CIRP). The members of the 2015 CIRP were Rehman Abdulrehman, PhD (Chair); Gonzalo Bacigalupe, EdD; Silvia S. Canetto, PhD; Amanda Clinton, PhD; Melissa L. Morgan Consoli, PhD; Chryse G. Hatzichristou, PhD; Arpana G. Inman, PhD; Lori Foster Thompson, PhD; and Danny Wedding, PhD. Dr. Abdulrehman, Dr. Morgan Consoli, Dr. Thompson, and Dr. Wedding were members of the subcommittee for the 2016 award. (PsycINFO Database Record

  11. But Dr. Meisels Is Not Convinced.

    Science.gov (United States)

    Meisels, Samuel J.

    1987-01-01

    Commenting on the Gesell Institute's response to his original article concerning the Gesell assessments, Dr. Meisels continues to maintain that the Gesell readiness tests lack sufficient proof of validity. (BB)

  12. Dr. Irvin Yalom Discusses Group Psychotherapy.

    Science.gov (United States)

    Forester-Miller, Holly

    1989-01-01

    In this interview, Dr. Irvin Yalom, director of the Adult Psychiatry Clinic at Stanford University School of Medicine, discusses his beginnings as a group psychotherapist, current issues in group work, and the future of group work. (Author/TE)

  13. Dr. Shi Hanzhang's Experience in Treating Andropathy

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    @@ Dr. Shi Hanzhang (施汉章) of Dongzhimen Hospital affiliated to Beijing University of Traditional Chinese Medicine is experienced in both teaching and clinical practice, especially in treating andropathy, such as sexual disorder, infertility, and hyperplasia of the prostate. The author has the honour to follow him for study and really learned a lot from him. A brief introduction to Dr. Shi's way of thinking in treatment is as follows.

  14. Implementing Python for DrRacket

    OpenAIRE

    Ramos, Pedro Palma; Leitão, António Menezes

    2014-01-01

    The Python programming language is becoming increasingly popular in a variety of areas, most notably among novice programmers. On the other hand, Racket and other Scheme dialects are considered excellent vehicles for introducing Computer Science concepts. This paper presents an implementation of Python for Racket and the DrRacket IDE. This allows Python programmers to use Racket libraries and vice versa, as well as using DrRacket's pedagogic features. In particular, it allows architects and d...

  15. Dr. Cheng Yichun's Experience in Treating Hyperthyroidism

    Institute of Scientific and Technical Information of China (English)

    Sun Fenglei; Feng Jianhua; Wang Xinzhong

    2006-01-01

    @@ Having been engaged in the TCM clinical practice for more than 40 years, Dr. Cheng Yichun from the Affiliated Hospital of Shandong University of TCM has rich experience in treating endocrine metabolic diseases. Authors have had the good fortune to follow Dr. Chen for 10 years. Following is his train of thought in diagnosing and treating hyperthyroidism and his prescriptions most in use for the disease.

  16. Sacroillite tuberculeuse: à propos de deux cas

    Science.gov (United States)

    Diallo, Ismaël; Zabsonré, Joëlle Tiendrébéogo; Kambou, Bénilde Marie Ange Tiemtoré; Sondo, Apoline Kongnimissom; Sagna, Yempabou; Ouédraogo, Dieu-Donné

    2016-01-01

    La sacroiliite tuberculeuse est rare et de diagnostic difficile. Les auteurs rapportent deux cas. Il s'agissait dans le premier cas d'une patiente de 40 ans ayant une infection à VIH ; le diagnostic a été histologique après une biopsie chirurgicale. Le second cas a concerné un patient de 25 ans vivant en milieu carcéral chez qui le diagnostic a été établi sur la base des arguments cliniques, biologiques, radiologiques et l'efficacité du traitement ; l'intradermoréaction à la tuberculine était phlycténulaire. Le scanner a été indispensable au diagnostic lésionnel en montrant une érosion des berges et des abcès des parties molles. Le traitement a été médical et a fait appel aux antituberculeux. PMID:28292032

  17. CAS physicist receives Tate Medal from American Institute of Physics

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    @@ The American Institute of Physics (AIP) 2007 Tate Medal for Leadership in International Physics goes to Prof. YU Lu, a physicist from the CAS Institute of Physics and chair of the CAS Interdisciplinary Center for Theoretical Studies.

  18. CAS to Further Strengthen Cooperation with the EU

    Institute of Scientific and Technical Information of China (English)

    2004-01-01

    @@ In light of the concept that scientific knowledge should serve humanity, CAS is open to the whole world and will further cooperate with the European Union in various fields, asserts CAS President Lu Yongxiang.

  19. CAS enacts rules to guide management of its institutes

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    @@ CAS issued Regulations for Comprehensive Administration of Research Institutes at the annual work conference opened on 24 March in Beijing. It is regarded as another cardinal document for CAS management after its Constitution, which was decreed in 2006.

  20. Evolution and classification of the CRISPR-Cas systems

    Science.gov (United States)

    S. Makarova, Kira; H. Haft, Daniel; Barrangou, Rodolphe; J. J. Brouns, Stan; Charpentier, Emmanuelle; Horvath, Philippe; Moineau, Sylvain; J. M. Mojica, Francisco; I. Wolf, Yuri; Yakunin, Alexander F.; van der Oost, John; V. Koonin, Eugene

    2012-01-01

    The CRISPR–Cas (clustered regularly interspaced short palindromic repeats–CRISPR-associated proteins) modules are adaptive immunity systems that are present in many archaea and bacteria. These defence systems are encoded by operons that have an extraordinarily diverse architecture and a high rate of evolution for both the cas genes and the unique spacer content. Here, we provide an updated analysis of the evolutionary relationships between CRISPR–Cas systems and Cas proteins. Three major types of CRISPR–Cas system are delineated, with a further division into several subtypes and a few chimeric variants. Given the complexity of the genomic architectures and the extremely dynamic evolution of the CRISPR–Cas systems, a unified classification of these systems should be based on multiple criteria. Accordingly, we propose a `polythetic' classification that integrates the phylogenies of the most common cas genes, the sequence and organization of the CRISPR repeats and the architecture of the CRISPR–cas loci. PMID:21552286

  1. CNPC and CAS Sign Technological Cooperation Agreement

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    @@ On August 16, 2006, China National Petroleum Corporation (CNPC) signed technological cooperation agreement with Chinese Academy of Sciences (CAS) at the Diaoyutai State Guesthouse in Beijing. Vice President of CNPC Zhou Jiping and Vice President of CAS Li Jiayang signed the agreement on behalf of both sides,marking a new era of unite brainstorm in petroleum technology. It will boost the development of petrochemical technology, especially the technology for the exploration and development of oil and gas field, engineering, oil refining and chemicals.

  2. CRISPR-Cas: Revolutionising genome engineering.

    Science.gov (United States)

    Nicholson, Samantha Anne; Pepper, Michael Sean

    2016-08-01

    The ability to permanently alter or repair the human genome has been the subject of a number of science fiction films, but with the recent advent of several customisable sequence-specific endonuclease technologies, genome engineering looks set to become a clinical reality in the near future. This article discusses recent advancements in the technology called 'clustered regularly interspaced palindromic repeat (CRISPR)-associated genes' (CRISPR-Cas), the potential of CRISPR-Cas to revolutionise molecular medicine, and the ethical and regulatory hurdles facing its application.

  3. CRISPR-Cas immunity in prokaryotes.

    Science.gov (United States)

    Marraffini, Luciano A

    2015-10-01

    Prokaryotic organisms are threatened by a large array of viruses and have developed numerous defence strategies. Among these, only clustered, regularly interspaced short palindromic repeat (CRISPR)-Cas systems provide adaptive immunity against foreign elements. Upon viral injection, a small sequence of the viral genome, known as a spacer, is integrated into the CRISPR locus to immunize the host cell. Spacers are transcribed into small RNA guides that direct the cleavage of the viral DNA by Cas nucleases. Immunization through spacer acquisition enables a unique form of evolution whereby a population not only rapidly acquires resistance to its predators but also passes this resistance mechanism vertically to its progeny.

  4. CAS scientists active in earthquake relief operation

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    @@ Soon after the devastating earthquake taking place on last 12 May in Sichuan, the CAS leadership promptly mobilized its researchers to dedicate their expertise to the nationwide drive of the disaster's relief and reduction. A leading group was established to coordinate the Academy's efforts in this regard. All of the eight CAS institutions located in Sichuan were organized to do everything possible to reduce their losses to the minimum. At the same time, a trans-departmental and interdisciplinary taskforce was formed to provide a powerful S&T backing for the nationwide drive.

  5. Psychological Treatment

    Science.gov (United States)

    ... IBS Pain IBS Global Treatments IBS Diet Low FODMAP Diet Complimentary or Alt Treatments Medications Psychological Treatments ... IBS Pain IBS Global Treatments IBS Diet Low FODMAP Diet Complimentary or Alt Treatments Medications Psychological Treatments ...

  6. Engineered CRISPR-Cas9 nucleases with altered PAM specificities.

    Science.gov (United States)

    Kleinstiver, Benjamin P; Prew, Michelle S; Tsai, Shengdar Q; Topkar, Ved V; Nguyen, Nhu T; Zheng, Zongli; Gonzales, Andrew P W; Li, Zhuyun; Peterson, Randall T; Yeh, Jing-Ruey Joanna; Aryee, Martin J; Joung, J Keith

    2015-07-23

    Although CRISPR-Cas9 nucleases are widely used for genome editing, the range of sequences that Cas9 can recognize is constrained by the need for a specific protospacer adjacent motif (PAM). As a result, it can often be difficult to target double-stranded breaks (DSBs) with the precision that is necessary for various genome-editing applications. The ability to engineer Cas9 derivatives with purposefully altered PAM specificities would address this limitation. Here we show that the commonly used Streptococcus pyogenes Cas9 (SpCas9) can be modified to recognize alternative PAM sequences using structural information, bacterial selection-based directed evolution, and combinatorial design. These altered PAM specificity variants enable robust editing of endogenous gene sites in zebrafish and human cells not currently targetable by wild-type SpCas9, and their genome-wide specificities are comparable to wild-type SpCas9 as judged by GUIDE-seq analysis. In addition, we identify and characterize another SpCas9 variant that exhibits improved specificity in human cells, possessing better discrimination against off-target sites with non-canonical NAG and NGA PAMs and/or mismatched spacers. We also find that two smaller-size Cas9 orthologues, Streptococcus thermophilus Cas9 (St1Cas9) and Staphylococcus aureus Cas9 (SaCas9), function efficiently in the bacterial selection systems and in human cells, suggesting that our engineering strategies could be extended to Cas9s from other species. Our findings provide broadly useful SpCas9 variants and, more importantly, establish the feasibility of engineering a wide range of Cas9s with altered and improved PAM specificities.

  7. North American Journal of Psychology, 1999.

    Science.gov (United States)

    McCutcheon, Lynn E., Ed.

    1999-01-01

    "North American Journal of Psychology" publishes scientific papers of general interest to psychologists and other social scientists. Articles included in volume 1 issue 1 (June 1999) are: "Generalist Looks at His Career in Teaching: Interview with Dr. Phil Zimbardo"; "Affective Information in Videos"; "Infant Communication"; "Defining Projective…

  8. Structural basis of LaDR5, a novel agonistic anti-death receptor 5 (DR5 monoclonal antibody, to inhibit DR5/TRAIL complex formation

    Directory of Open Access Journals (Sweden)

    Qiao Chunxia

    2012-07-01

    Full Text Available Abstract Background As a member of the TNF superfamily, TRAIL could induce human tumor cell apoptosis through its cognate death receptors DR4 or DR5, which can induce formation of the death inducing signaling complex (DISC and activation of the membrane proximal caspases (caspase-8 or caspase-10 and mitochondrial pathway. Some monoclonal antibodies against DR4 or DR5 have been reported to have anti-tumor activity. Results In this study, we reported a novel mouse anti-human DR5 monoclonal antibody, named as LaDR5, which could compete with TRAIL to bind DR5 and induce the apoptosis of Jurkat cells in the absence of second cross-linking in vitro. Using computer-guided molecular modeling method, the 3-D structure of LaDR5 Fv fragment was constructed. According to the crystal structure of DR5, the 3-D complex structure of DR5 and LaDR5 was modeled using molecular docking method. Based on distance geometry method and intermolecular hydrogen bonding analysis, the key functional domain in DR5 was predicted and the DR5 mutants were designed. And then, three mutants of DR5 was expressed in prokaryotic system and purified by affinity chromatograph to determine the epitope of DR5 identified by LaDR5, which was consistent with the theoretical results of computer-aided analysis. Conclusions Our results demonstrated the specific epitope located in DR5 that plays a crucial role in antibody binding and even antineoplastic bioactivity. Meanwhile, revealed structural features of DR5 may be important to design or screen novel drugs agonist DR5.

  9. Discursive Psychology

    NARCIS (Netherlands)

    Molder, te H.

    2015-01-01

    Discursive psychology was established in the United Kingdom by the end of the 1980s, mainly in response to the dominant cognitivist approach in social psychology. While it borrowed notions from poststructuralism and sociology of science, it is most akin to conversation analysis. Discursive psycholog

  10. Positive Psychology

    Science.gov (United States)

    Peterson, Christopher

    2009-01-01

    Positive psychology is a deliberate correction to the focus of psychology on problems. Positive psychology does not deny the difficulties that people may experience but does suggest that sole attention to disorder leads to an incomplete view of the human condition. Positive psychologists concern themselves with four major topics: (1) positive…

  11. CAS Maps Out a Blueprint for Its Future Development

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    @@ At the enlarged summer session of the Party's Leading Group at CAS held from July 25 to 29 in Beijing,the CAS leadership called for implementing the third-phase work of the Knowledge Innovation Program (KIP) through innovation and reform, so as to open up new prospects for CAS development in the years to come.

  12. CAS, Thai S&T ministry to further cooperation

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    @@ Hosted by the CAS Bureau of International Cooperation, the Second CAS-Thai MOST Joint Workshop on S&T Cooperation was held on 28 and 29 June in Chengdu, capital of southwest China's Sichuan Province. More than 120 officials and scholars from Sichuan Provincial Government, CAS, and Thai Ministry of Science and Technology(MOST) were present at the meeting.

  13. Structural plasticity and in vivo activity of Cas1 from the type I-F CRISPR-Cas system.

    Science.gov (United States)

    Wilkinson, Max E; Nakatani, Yoshio; Staals, Raymond H J; Kieper, Sebastian N; Opel-Reading, Helen K; McKenzie, Rebecca E; Fineran, Peter C; Krause, Kurt L

    2016-04-15

    CRISPR-Cas systems are adaptive immune systems in prokaryotes that provide protection against viruses and other foreign DNA. In the adaptation stage, foreign DNA is integrated into CRISPR (clustered regularly interspaced short palindromic repeat) arrays as new spacers. These spacers are used in the interference stage to guide effector CRISPR associated (Cas) protein(s) to target complementary foreign invading DNA. Cas1 is the integrase enzyme that is central to the catalysis of spacer integration. There are many diverse types of CRISPR-Cas systems, including type I-F systems, which are typified by a unique Cas1-Cas2-3 adaptation complex. In the present study we characterize the Cas1 protein of the potato phytopathogen Pectobacterium atrosepticum, an important model organism for understanding spacer acquisition in type I-F CRISPR-Cas systems. We demonstrate by mutagenesis that Cas1 is essential for adaptation in vivo and requires a conserved aspartic acid residue. By X-ray crystallography, we show that although P. atrosepticum Cas1 adopts a fold conserved among other Cas1 proteins, it possesses remarkable asymmetry as a result of structural plasticity. In particular, we resolve for the first time a flexible, asymmetric loop that may be unique to type I-F Cas1 proteins, and we discuss the implications of these structural features for DNA binding and enzymatic activity.

  14. CRISPR-Cas9 gene editing

    NARCIS (Netherlands)

    Oude Blenke, Erik; Evers, Martijn J.W.; Mastrobattista, Enrico; Oost, van der John

    2016-01-01

    The CRISPR-Cas9 gene editing system has taken the biomedical science field by storm, initiating rumors about future Nobel Prizes and heating up a fierce patent war, but also making significant scientific impact. The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR), together with

  15. CRISPR-Cas9 gene editing

    NARCIS (Netherlands)

    Oude Blenke, Erik; Evers, Martijn J.W.; Mastrobattista, Enrico; Oost, van der John

    2016-01-01

    The CRISPR-Cas9 gene editing system has taken the biomedical science field by storm, initiating rumors about future Nobel Prizes and heating up a fierce patent war, but also making significant scientific impact. The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR), together with

  16. CAS scores progress in MIS development

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    @@ A CAS initiative to upgrade its management information system (MIS) is making landmark progress. The first-phase development of the Academia Resource Planning (ARP), a major MIS project at the Academy, passed the acceptance check by a 17-strong panel of experts on 23 January 2007 in Beijing.

  17. CAS expert receives Tribology Gold Medal

    Institute of Scientific and Technical Information of China (English)

    2012-01-01

    Prof. XUE Qunji, a senior expert in tribology from the CAS Lanzhou Institute of Chemical Physics (L1CP) and member of the Chinese Academy of Engineering, received the Tribology Gold Medalfor 2011 on February 27, 2012 in Beijing. As one of the world's most outstanding and influential tribologists in the last forty years, Prof.

  18. CAS researchers find a new mineral

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    @@ Mineralogists from the CAS Guangzhou Institute of Geochemistry (GIG) recently discovered Xieite, a chromium-iron oxide in its natural state. It has been authorized as a new mineral by Commission on New Minerals Nomenclature and Classification under the International Mineralogical Association (CNMNC-IMA).

  19. Using the CAS Standards in Assessment Projects

    Science.gov (United States)

    Dean, Laura A.

    2013-01-01

    This chapter provides an overview of the use of professional standards of practice in assessment and of the Council for the Advancement of Standards in Higher Education (CAS). It outlines a model for conducting program self-studies and discusses the importance of implementing change based on assessment results.

  20. Boosting plant immunity with CRISPR/Cas

    OpenAIRE

    Chaparro-Garcia, Angela; Kamoun, Sophien; Nekrasov, Vladimir

    2015-01-01

    CRISPR/Cas has recently been transferred to plants to make them resistant to geminiviruses, a damaging family of DNA viruses. We discuss the potential and the limitations of this method.See related Research: http://www.genomebiology.com/2015/16/1/238.

  1. Boosting plant immunity with CRISPR/Cas.

    Science.gov (United States)

    Chaparro-Garcia, Angela; Kamoun, Sophien; Nekrasov, Vladimir

    2015-11-19

    CRISPR/Cas has recently been transferred to plants to make them resistant to geminiviruses, a damaging family of DNA viruses. We discuss the potential and the limitations of this method.See related Research: http://www.genomebiology.com/2015/16/1/238.

  2. Dræb, dræb, dræb! Nej ... liiiige et øjeblik: De machiavelliske følelser i Game of Thrones

    DEFF Research Database (Denmark)

    Schubart, Rikke

    2013-01-01

    Blogindlæg om Machiavelliske følelser i HBO tv-serien Game of Thrones: "Dræb, dræb, dræb! Nej ... liiiige et øjeblik: De machiavelliske følelser i Game of Thrones"......Blogindlæg om Machiavelliske følelser i HBO tv-serien Game of Thrones: "Dræb, dræb, dræb! Nej ... liiiige et øjeblik: De machiavelliske følelser i Game of Thrones"...

  3. Geophysical survey of 105-DR Pluto Crib, 116-DR-4, 100-D Area

    Energy Technology Data Exchange (ETDEWEB)

    Bergstrom, K.A.

    1993-10-01

    The objective of this Geophysical Survey was to verify the location of the 105-DR Pluto Crib, 116-DR-4. A surface monument currently marks its location. The crib is 10 feet by 10 feet and 15 feet deep. Ground-Penetrating Radar was the geophysical method selected to conduct the investigation.

  4. Dr. David Syz, State Secretary for Economic Affairs, Switzerland

    CERN Multimedia

    Patrice Loïez

    2003-01-01

    Photo 01: Dr. David Syz (left) with Professor Roger Cashmore, Research Director for Collider Programmes.Photo 02: Dr. David Syz signing the VIP visitors' book, with Prof. Roger Cashmore.Photo 03: Dr. David Syz signing the VIP visitors' book.Photo 04: Handshake between Dr. David Syz (left) and Prof. Roger Cashmore.

  5. Controlling UCAVs by JTACs in CAS missions

    Science.gov (United States)

    Kumaş, A. E.

    2014-06-01

    By means of evolving technology, capabilities of UAVs (Unmanned Aerial Vehicle)s are increasing rapidly. This development provides UAVs to be used in many different areas. One of these areas is CAS (Close Air Support) mission. UAVs have several advantages compared to manned aircraft, however there are also some problematic areas. The remote controlling of these vehicles from thousands of nautical miles away via satellite may lead to various problems both ethical and tactical aspects. Therefore, CAS missions require a good level of ALI (Air-Land Integration), a high SA (situational awareness) and precision engagement. In fact, there is an aware friendly element in the target area in CAS missions, unlike the other UAV operations. This element is an Airman called JTAC (Joint Terminal Attack Controller). Unlike the JTAC, UAV operators are too far away from target area and use the limited FOV (Field of View) provided by camera and some other sensor data. In this study, target area situational awareness of a UAV operator and a JTAC, in a high-risk mission for friendly ground forces and civilians such as CAS, are compared. As a result of this comparison, answer to the question who should control the UCAV (Unmanned Combat Aerial Vehicle) in which circumstances is sought. A literature review is made in UAV and CAS fields and recent air operations are examined. The control of UCAV by the JTAC is assessed by SWOT analysis and as a result it is deduced that both control methods can be used in different situations within the framework of the ROE (Rules Of Engagement) is reached.

  6. Response to the editorial by Dr Geraghty.

    Science.gov (United States)

    White, Peter D; Chalder, Trudie; Sharpe, Michael; Angus, Brian J; Baber, Hannah L; Bavinton, Jessica; Burgess, Mary; Clark, Lucy V; Cox, Diane L; DeCesare, Julia C; Goldsmith, Kimberley A; Johnson, Anthony L; McCrone, Paul; Murphy, Gabrielle; Murphy, Maurice; O'Dowd, Hazel; Potts, Laura; Walwyn, Rebacca; Wilks, David

    2017-08-01

    This article is written in response to the linked editorial by Dr Geraghty about the adaptive Pacing, graded Activity and Cognitive behaviour therapy; a randomised Evaluation (PACE) trial, which we led, implemented and published. The PACE trial compared four treatments for people diagnosed with chronic fatigue syndrome. All participants in the trial received specialist medical care. The trial found that adding cognitive behaviour therapy or graded exercise therapy to specialist medical care was as safe as, and more effective than, adding adaptive pacing therapy or specialist medical care alone. Dr Geraghty has challenged these findings. In this article, we suggest that Dr Geraghty's views are based on misunderstandings and misrepresentations of the PACE trial; these are corrected.

  7. Naturally Occurring Off-Switches for CRISPR-Cas9.

    Science.gov (United States)

    Pawluk, April; Amrani, Nadia; Zhang, Yan; Garcia, Bianca; Hidalgo-Reyes, Yurima; Lee, Jooyoung; Edraki, Alireza; Shah, Megha; Sontheimer, Erik J; Maxwell, Karen L; Davidson, Alan R

    2016-12-15

    CRISPR-Cas9 technology would be enhanced by the ability to inhibit Cas9 function spatially, temporally, or conditionally. Previously, we discovered small proteins encoded by bacteriophages that inhibit the CRISPR-Cas systems of their host bacteria. These "anti-CRISPRs" were specific to type I CRISPR-Cas systems that do not employ the Cas9 protein. We posited that nature would also yield Cas9 inhibitors in response to the evolutionary arms race between bacteriophages and their hosts. Here, we report the discovery of three distinct families of anti-CRISPRs that specifically inhibit the CRISPR-Cas9 system of Neisseria meningitidis. We show that these proteins bind directly to N. meningitidis Cas9 (NmeCas9) and can be used as potent inhibitors of genome editing by this system in human cells. These anti-CRISPR proteins now enable "off-switches" for CRISPR-Cas9 activity and provide a genetically encodable means to inhibit CRISPR-Cas9 genome editing in eukaryotes. VIDEO ABSTRACT. Copyright © 2016 Elsevier Inc. All rights reserved.

  8. Nucleosome breathing and remodeling constrain CRISPR-Cas9 function.

    Science.gov (United States)

    Isaac, R Stefan; Jiang, Fuguo; Doudna, Jennifer A; Lim, Wendell A; Narlikar, Geeta J; Almeida, Ricardo

    2016-04-28

    The CRISPR-Cas9 bacterial surveillance system has become a versatile tool for genome editing and gene regulation in eukaryotic cells, yet how CRISPR-Cas9 contends with the barriers presented by eukaryotic chromatin is poorly understood. Here we investigate how the smallest unit of chromatin, a nucleosome, constrains the activity of the CRISPR-Cas9 system. We find that nucleosomes assembled on native DNA sequences are permissive to Cas9 action. However, the accessibility of nucleosomal DNA to Cas9 is variable over several orders of magnitude depending on dynamic properties of the DNA sequence and the distance of the PAM site from the nucleosome dyad. We further find that chromatin remodeling enzymes stimulate Cas9 activity on nucleosomal templates. Our findings imply that the spontaneous breathing of nucleosomal DNA together with the action of chromatin remodelers allow Cas9 to effectively act on chromatin in vivo.

  9. An interview with Dr Barbara A. Carper.

    Science.gov (United States)

    Eisenhauer, Elizabeth R

    2015-01-01

    In 1978, Dr Barbara A. Carper's foundational work, "Fundamental Patterns of Knowing in Nursing," arguably created a paradigm shift in nursing. However, her voice has been absent from the nursing literature in recent years. I was privileged to conduct a personal interview with Dr Carper in 2014. The edited interview includes a synopsis of her background, career trajectory, sources of inspiration, and her perspective on the current state of nursing. She reaffirmed her passion for reflective nursing practice, the importance incorporating the arts and humanities into nursing education, and using an integrated approach with the patterns of knowing in nursing.

  10. Unification of Cas protein families and a simple scenario for the origin and evolution of CRISPR-Cas systems

    Directory of Open Access Journals (Sweden)

    Wolf Yuri I

    2011-07-01

    Full Text Available Abstract Background The CRISPR-Cas adaptive immunity systems that are present in most Archaea and many Bacteria function by incorporating fragments of alien genomes into specific genomic loci, transcribing the inserts and using the transcripts as guide RNAs to destroy the genome of the cognate virus or plasmid. This RNA interference-like immune response is mediated by numerous, diverse and rapidly evolving Cas (CRISPR-associated proteins, several of which form the Cascade complex involved in the processing of CRISPR transcripts and cleavage of the target DNA. Comparative analysis of the Cas protein sequences and structures led to the classification of the CRISPR-Cas systems into three Types (I, II and III. Results A detailed comparison of the available sequences and structures of Cas proteins revealed several unnoticed homologous relationships. The Repeat-Associated Mysterious Proteins (RAMPs containing a distinct form of the RNA Recognition Motif (RRM domain, which are major components of the CRISPR-Cas systems, were classified into three large groups, Cas5, Cas6 and Cas7. Each of these groups includes many previously uncharacterized proteins now shown to adopt the RAMP structure. Evidence is presented that large subunits contained in most of the CRISPR-Cas systems could be homologous to Cas10 proteins which contain a polymerase-like Palm domain and are predicted to be enzymatically active in Type III CRISPR-Cas systems but inactivated in Type I systems. These findings, the fact that the CRISPR polymerases, RAMPs and Cas2 all contain core RRM domains, and distinct gene arrangements in the three types of CRISPR-Cas systems together provide for a simple scenario for origin and evolution of the CRISPR-Cas machinery. Under this scenario, the CRISPR-Cas system originated in thermophilic Archaea and subsequently spread horizontally among prokaryotes. Conclusions Because of the extreme diversity of CRISPR-Cas systems, in-depth sequence and structure

  11. Psychological Empowerment

    OpenAIRE

    Flohrer, Nick

    2014-01-01

    Instilling psychological empowerment in employees is one of the most important tasks of modern leadership. Building on quantitative research and the development of a new psychometric scale related to project management this thesis shows: First, individuals' characteristics and their work team environment influence perceptions of access to information and resources – two important antecedents of psychological empowerment. Second, while a project briefing strengthens the link of the psychologic...

  12. [Political psychology].

    Science.gov (United States)

    Resch, Mária; Bella, Tamás

    2013-04-21

    In Hungary one can mostly find references to the psychological processes of politics in the writings of publicists, public opinion pollsters, philosophers, social psychologists, and political analysts. It would be still important if not only legal scientists focusing on political institutions or sociologist-politologists concentrating on social structures could analyse the psychological aspects of political processes; but one could also do so through the application of the methods of political psychology. The authors review the history of political psychology, its position vis-à-vis other fields of science and the essential interfaces through which this field of science, which is still to be discovered in Hungary, connects to other social sciences. As far as its methodology comprising psycho-biographical analyses, questionnaire-based queries, cognitive mapping of interviews and statements are concerned, it is identical with the psychiatric tools of medical sciences. In the next part of this paper, the focus is shifted to the essence and contents of political psychology. Group dynamics properties, voters' attitudes, leaders' personalities and the behavioural patterns demonstrated by them in different political situations, authoritativeness, games, and charisma are all essential components of political psychology, which mostly analyses psychological-psychiatric processes and also involves medical sciences by relying on cognitive and behavioural sciences. This paper describes political psychology, which is basically part of social sciences, still, being an interdisciplinary science, has several ties to medical sciences through psychological and psychiatric aspects.

  13. In Memoriam- Prof. Dr. Andries Willem Lategan

    Directory of Open Access Journals (Sweden)

    S. P. Ligthelm

    1965-03-01

    Full Text Available Hierdie Curriculum Vitae sou voorgelees gewees het met die inougurele rede van wyle prof. dr. A. W. Lategan met die aanvaarding van 'n ereprofessoraat aan die P.U. vir C.H.O. op 29 Oktober 1965. Prof. Lategan is op 23 Oktober 1965 oor- lcde. Sy inougurele rede verskyn as ’n bylaag in hierdie uit- gawe.

  14. Dr. Strangelove and Irrational Collective Rationality

    Institute of Scientific and Technical Information of China (English)

    韩蕾

    2012-01-01

      Dr. Strangelove or: How I learned to Stop Worrying and Love the Bomb is a 1964 black comedy film with satire of arm races and nuclear scare by the director Stanley Kubrick. In the movie, irrational practice and ideologies manifest them⁃selves as collective rationality and result in the destruction of the earth.

  15. Dr. A.F.M. Reijnders, centenarian

    NARCIS (Netherlands)

    Bas, C.

    1999-01-01

    A few weeks ago a reprint was distributed of an interesting paper on the formation of spores by metamorphosis of basidia in fruiting bodies of Mycocalia and Scleroderma. That in itself was not so remarkable. But it is really a small miracle that this paper was written by Dr. A.F.M. Reijnders at the

  16. Dr D. F. van Slooten †

    NARCIS (Netherlands)

    Lam, H.J.

    1953-01-01

    On March 7, 1953 the Rijksherbarium suffered a severe loss by the sudden death of its honorary co-operator Dr. D. F. van Slooten, the wellknown specialist particularly of Dipterocarpaceae. To honour his memory the following lines may serve as an obituary note. Dirk Fok van Slooten was born in Amersf

  17. ["Allergy testing" with "Dr. Voll electroacupuncture"].

    Science.gov (United States)

    Bresser, H

    1993-06-01

    Electroacupuncture according to Dr. Voll (EAV) is one of the numerous unconventional methods propagated for allergy testing in Germany. From an experimental examination for "drug testing" of this method, it can be concluded that EAV is unsuitable for any form of allergy testing.

  18. Dr. Lutz Reichhoff zum 65. Geburtstag

    OpenAIRE

    Böhnert, Wolfgang; Schönbrodt, Robert

    2014-01-01

    Am 26. Oktober 2013 vollendete Dr. rer. nat. sc. Lutz Reichhoff sein 65. Lebensjahr. Alle die ihn näher kennen wissen, dass diese Jahreszahl noch lange nicht seinen Ruhestand einläuten wird. Wir nehmen dennoch gern diesen Tag zum Anlass, seine umfangreichen und vielfältigen Leistungen im beruflichen und ehrenamtlichen Wirken zu würdigen.

  19. Walt Disney and Dr. Wernher von Braun

    Science.gov (United States)

    1954-01-01

    Dr. Werhner von Braun, then Chief, Guided Missile Development Operation Division at Army Ballistic Missile Agency (ABMA) in Redstone Arsenal, Alabama, was visited by Walt Disney in 1954. In the 1950's, von Braun worked with Disney Studio as a technical director, making three films about space exploration for television. A model of the V-2 rocket is in background.

  20. Dr. S. J. van Ooststroom retired

    NARCIS (Netherlands)

    Steenis, van C.G.G.J.

    1971-01-01

    In January 1971 Dr. Simon Jan van Ooststroom, senior botanist of the Rijksherbarium, retired on reaching the age of 65, having been on the staff since November 1934. Though this event will to a certain extent change, but not interrupt, his work, it is nevertheless worth commemorating, as he has so

  1. Stop words for “Dr Math”

    CSIR Research Space (South Africa)

    Butgereit, L

    2011-05-01

    Full Text Available “Dr Math” is a facility where primary and secondary school pupils can use MXit on their cell phones to get help with their mathematics homework. Pupils use an abbreviated “MXit lingo” leaving out most vowels and substituting various numerals...

  2. Confessions of a Dr Math tutor

    CSIR Research Space (South Africa)

    Butgereit, L

    2015-06-01

    Full Text Available Mathematics look different on a small 3-inch screen of an inexpensive cell phone when compared to a 3-meter whiteboard in a mathematics classroom. Dr Math uses cell phone or mobile data "chat" technologies to assist primary and secondary school...

  3. Dr. Bakhuizen van den Brink retires

    NARCIS (Netherlands)

    Kalkman, C.

    1976-01-01

    On 11 September 1976 dr. Reinier Cornelis Bakhuizen van den Brink will reach the age of 65 and so he must retire from his position as a senior botanist at the Rijksherbarium. He has worked in our institute since December 1943, after having obtained his doctor’s degree in Utrecht. At the Rijksherbari

  4. Non-gyroscope DR and adaptive information fusion algorithm used in GPS/DR device

    Institute of Scientific and Technical Information of China (English)

    Li Qingli; Xue Yongqi; Shang Yanlei; Shi Pengfei

    2006-01-01

    In view of the problems existing in GPS, a non-gyroscope DR is introduced. The operating principle and the algorithm of the GPS/DR device are also presented. By operating measured data synthetically, linear observation equations are obtained for the information fusion algorithm. This approach avoids model error due to linearizing nonlinear observation equations in the conventional algorithm, so that the stability of information fusion algorithm is improved and computation expenses are reduced. Field running experiments show that satisfactory accuracy can be obtained by the proposed navigation model and algorithm for the non-gyroscope GPS/DR device.

  5. Coverage of Russian psychological contributions in American psychology textbooks.

    Science.gov (United States)

    Aleksandrova-Howell, Maria; Abramson, Charles I; Craig, David Philip Arthur

    2012-01-01

    Internationalizing psychology is an important component of current globalization trends. American textbooks on the history of psychology and introductory psychology were surveyed for the presence of historical and contemporary important Russian psychologists to assess the current status of Russian-American crossfertilization. Of a list of 97 important Russian psychologists, as determined by the editors of the Russian journal Methodology and History in Psychology, less than 22% are mentioned in the reviewed texts. The most common names were Pavlov, Luria, and Vygotsky. As the internet is arguably the single most important factor affecting the increase of international communication and dissemination of knowledge, we also searched for these 97 names on various websites, most notably Wikipedia and Google. Forty-one internet sites contained some amount of biographical information about Russian psychologists. On Wikipedia, 14 Russian psychologists had articles documenting biographical information. We also developed a rubric to determine the amount of information available on the internet for these psychologists and compared Wikipedia's mean score with various other websites. Wikipedia pages on average had a significantly higher score than the rest of the internet. Recommendations to improve Russian coverage in America are provided and include: (1) developing pages on Wikipedia and other virtual venues highlighting Russian contributions, (2) soliciting articles for US journals from Russian psychologists, and (3) incorporating Russian contributions in introductory and historical textbooks. We provide a partial bibliography of Russian contributions that can be used by authors of such textbooks. We would like to thank Dr Viktor Fedorovich Petrenko and Dr Igor Nikolaevich Karitsky from the journal Methodology and History of Psychology for supplying the names of the Russian psychologists. We would also like to express our appreciation to Robert García for reviewing and

  6. CAS Introduction to Accelerator Physics in Bulgaria

    CERN Multimedia

    CERN Bulletin

    2010-01-01

    The CERN Accelerator School (CAS) and the Institute for Nuclear Research & Nuclear Energy (INRNE – Bulgarian Academy of Sciences) jointly organised a course on Introduction to Accelerators, at the Grand Hotel Varna, Bulgaria, from 19 September to 1 October, 2010.   CERN Accelerator School group photo. The course was extremely well attended with 109 participants representing 34 different nationalities, coming from countries as far away as Australia, Canada and Vietnam. The intensive programme comprised 39 lectures, 3 seminars, 4 tutorials where the students were split into three groups, a poster session where students could present their own work, and 7 hours of guided and private study. Feedback from the participants was extremely positive, praising the expertise and enthusiasm of the lecturers, as well as the high standard and excellent quality of their lectures. For the first time at CAS, the CERN Director-General, Rolf Heuer, visited the school and presented a seminar entitled...

  7. CAS - Great success for the DSP course

    CERN Multimedia

    2007-01-01

    The CERN Accelerator School (CAS) and the Uppsala University jointly organized a specialized school on "Digital Signal Processing" in Sigtuna, Sweden from 1-9 June, 2007. This course was a "première" in many ways: firstly the topic had never been addressed by CAS, and secondly the structure of the course differed from the usual specialized courses in the sense that it was composed of 32 hours of theoretical lectures in the mornings and 16 hours "hands-on" courses in the afternoons. The latter, which have been designed by CERN experts, had some logistic implications in transporting computers and circuit boards (DSP and FPGA) to Sweden. The principle of this new approach was extremely well received by the accelerator community and 97 participants representing 23 different nationalities (80% of the participants originating from the CERN Member States) attended the course. As illustrated by the very positive feedback received from th...

  8. Efficient Mitochondrial Genome Editing by CRISPR/Cas9

    Directory of Open Access Journals (Sweden)

    Areum Jo

    2015-01-01

    Full Text Available The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR/Cas9 system has been widely used for nuclear DNA editing to generate mutations or correct specific disease alleles. Despite its flexible application, it has not been determined if CRISPR/Cas9, originally identified as a bacterial defense system against virus, can be targeted to mitochondria for mtDNA editing. Here, we show that regular FLAG-Cas9 can localize to mitochondria to edit mitochondrial DNA with sgRNAs targeting specific loci of the mitochondrial genome. Expression of FLAG-Cas9 together with gRNA targeting Cox1 and Cox3 leads to cleavage of the specific mtDNA loci. In addition, we observed disruption of mitochondrial protein homeostasis following mtDNA truncation or cleavage by CRISPR/Cas9. To overcome nonspecific distribution of FLAG-Cas9, we also created a mitochondria-targeted Cas9 (mitoCas9. This new version of Cas9 localizes only to mitochondria; together with expression of gRNA targeting mtDNA, there is specific cleavage of mtDNA. MitoCas9-induced reduction of mtDNA and its transcription leads to mitochondrial membrane potential disruption and cell growth inhibition. This mitoCas9 could be applied to edit mtDNA together with gRNA expression vectors without affecting genomic DNA. In this brief study, we demonstrate that mtDNA editing is possible using CRISPR/Cas9. Moreover, our development of mitoCas9 with specific localization to the mitochondria should facilitate its application for mitochondrial genome editing.

  9. Annotation and Classification of CRISPR-Cas Systems.

    Science.gov (United States)

    Makarova, Kira S; Koonin, Eugene V

    2015-01-01

    The clustered regularly interspaced short palindromic repeats (CRISPR)-Cas (CRISPR-associated proteins) is a prokaryotic adaptive immune system that is represented in most archaea and many bacteria. Among the currently known prokaryotic defense systems, the CRISPR-Cas genomic loci show unprecedented complexity and diversity. Classification of CRISPR-Cas variants that would capture their evolutionary relationships to the maximum possible extent is essential for comparative genomic and functional characterization of this theoretically and practically important system of adaptive immunity. To this end, a multipronged approach has been developed that combines phylogenetic analysis of the conserved Cas proteins with comparison of gene repertoires and arrangements in CRISPR-Cas loci. This approach led to the current classification of CRISPR-Cas systems into three distinct types and ten subtypes for each of which signature genes have been identified. Comparative genomic analysis of the CRISPR-Cas systems in new archaeal and bacterial genomes performed over the 3 years elapsed since the development of this classification makes it clear that new types and subtypes of CRISPR-Cas need to be introduced. Moreover, this classification system captures only part of the complexity of CRISPR-Cas organization and evolution, due to the intrinsic modularity and evolutionary mobility of these immunity systems, resulting in numerous recombinant variants. Moreover, most of the cas genes evolve rapidly, complicating the family assignment for many Cas proteins and the use of family profiles for the recognition of CRISPR-Cas subtype signatures. Further progress in the comparative analysis of CRISPR-Cas systems requires integration of the most sensitive sequence comparison tools, protein structure comparison, and refined approaches for comparison of gene neighborhoods.

  10. Breaking-Cas-interactive design of guide RNAs for CRISPR-Cas experiments for ENSEMBL genomes.

    Science.gov (United States)

    Oliveros, Juan C; Franch, Mònica; Tabas-Madrid, Daniel; San-León, David; Montoliu, Lluis; Cubas, Pilar; Pazos, Florencio

    2016-07-08

    The CRISPR/Cas technology is enabling targeted genome editing in multiple organisms with unprecedented accuracy and specificity by using RNA-guided nucleases. A critical point when planning a CRISPR/Cas experiment is the design of the guide RNA (gRNA), which directs the nuclease and associated machinery to the desired genomic location. This gRNA has to fulfil the requirements of the nuclease and lack homology with other genome sites that could lead to off-target effects. Here we introduce the Breaking-Cas system for the design of gRNAs for CRISPR/Cas experiments, including those based in the Cas9 nuclease as well as others recently introduced. The server has unique features not available in other tools, including the possibility of using all eukaryotic genomes available in ENSEMBL (currently around 700), placing variable PAM sequences at 5' or 3' and setting the guide RNA length and the scores per nucleotides. It can be freely accessed at: http://bioinfogp.cnb.csic.es/tools/breakingcas, and the code is available upon request.

  11. Prospects of CAS/MPS Cooperation

    Institute of Scientific and Technical Information of China (English)

    Mu Rongping

    2004-01-01

    @@ In the next 20 years, collaboration between the Chinese Academy of Sciences (CAS) and the Max Planck Society for the Advancement of Science (MPS) will meet with great strategic opportunities. We should seek the chance to innovatively improve our cooperation. This could include further improvement of the mechanisms,expansion of areas, actively seeking new patterns, and strengthening our research on science and technology foresight.

  12. CAS Wildlife Conservationist Receives Whitley Award

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    @@ Prof. JIANG Zhigang of the CAS Institute of Zoology has been honored with the 2006 Whitley Award by Whitley Fund for Nature, a UK registered charity offering a wide range of awards to outstanding nature conservation leaders around the world.Jiang is chosen for the award in recognition of his ecosystem approach to conservation of the Przewalski's Gazelle in pastoral areas around Qinghai Lake in northwest China's Qinghai-Tibet Plateau.

  13. CAS overseas talent programs pay off

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    @@ With an objective to establish a highly innovative research contingent,CAS has Iaunched a number of talent-recruitment and training schemes sinece the initiation of its Knowledge Innovation Program in 1998,namely,Engaging Outstanding Overseas Talents,Overseas Review Experts,Overseas Supervisors for Doctoral Candidates,Fund for Overseas Outstanding Scholars,Academic Seminars,Einstein Visiting Professorship,and Research Fellowship for International Young Researchers.

  14. A Sweeping Reshuffle at CAS Institutes

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    @@ Reorganization of CAS institutes was conducted over the past four years. For example, with the merger of the former Institute of Mathematics,Institute of Applied Mathematics, Institute of Systems Science and Institute of Computational Mathematics & Scientific/Engineering Computing, an Academy of Mathematics & System Sciences was formed. In the Shanghai area, the eight former biological institutes was regrouped into four institutes and one center,which formed the newly established Shanghai Institutes for Biological Sciences.

  15. Psychological behaviorism and behaviorizing psychology

    Science.gov (United States)

    Staats, Arthur W.

    1994-01-01

    Paradigmatic or psychological behaviorism (PB), in a four-decade history of development, has been shaped by its goal, the establishment of a behaviorism that can also serve as the approach in psychology (Watson's original goal). In the process, PB has become a new generation of behaviorism with abundant heuristic avenues for development in theory, philosophy, methodology, and research. Psychology has resources, purview and problem areas, and nascent developments of many kinds, gathered in chaotic diversity, needing unification (and other things) that cognitivism cannot provide. Behaviorism can, within PB's multilevel framework for connecting and advancing both psychology and behaviorism. PMID:22478175

  16. Psychological behaviorism and behaviorizing psychology.

    Science.gov (United States)

    Staats, A W

    1994-01-01

    Paradigmatic or psychological behaviorism (PB), in a four-decade history of development, has been shaped by its goal, the establishment of a behaviorism that can also serve as the approach in psychology (Watson's original goal). In the process, PB has become a new generation of behaviorism with abundant heuristic avenues for development in theory, philosophy, methodology, and research. Psychology has resources, purview and problem areas, and nascent developments of many kinds, gathered in chaotic diversity, needing unification (and other things) that cognitivism cannot provide. Behaviorism can, within PB's multilevel framework for connecting and advancing both psychology and behaviorism.

  17. Combination of TRAIL with bortezomib shifted apoptotic signaling from DR4 to DR5 death receptor by selective internalization and degradation of DR4.

    Directory of Open Access Journals (Sweden)

    Maxim L Bychkov

    Full Text Available TRAIL (tumor necrosis factor-related apoptosis-inducing ligand mediates apoptosis in cancer cells through death receptors DR4 and DR5 preferring often one receptor over another in the cells expressing both receptors. Receptor selective mutant variants of TRAIL and agonistic antibodies against DR4 and DR5 are highly promising anticancer agents. Here using DR5 specific mutant variant of TRAIL--DR5-B we have demonstrated for the first time that the sensitivity of cancer cells can be shifted from one TRAIL death receptor to another during co-treatment with anticancer drugs. First we have studied the contribution of DR4 and DR5 in HCT116 p53+/+ and HCT116 p53-/- cells and demonstrated that in HCT116 p53+/+ cells the both death receptors are involved in TRAIL-induced cell death while in HCT116 p53-/- cells prevailed DR4 signaling. The expression of death (DR4 and DR5 as well as decoy (DcR1 and DcR2 receptors was upregulated in the both cell lines either by TRAIL or by bortezomib. However, combined treatment of cells with two drugs induced strong time-dependent and p53-independent internalization and further lysosomal degradation of DR4 receptor. Interestingly DR5-B variant of TRAIL which do not bind with DR4 receptor also induced elimination of DR4 from cell surface in combination with bortezomib indicating the ligand-independent mechanism of the receptor internalization. Eliminatory internalization of DR4 resulted in activation of DR5 receptor thus DR4-dependent HCT116 p53-/- cells became highly sensitive to DR5-B in time-dependent manner. Internalization and degradation of DR4 receptor depended on activation of caspases as well as of lysosomal activity as it was completely inhibited by Z-VAD-FMK, E-64 and Baf-A1. In light of our findings, it is important to explore carefully which of the death receptors is active, when sensitizing drugs are combined with agonistic antibodies to the death receptors or receptor selective variants of TRAIL to enhance

  18. Combination of TRAIL with bortezomib shifted apoptotic signaling from DR4 to DR5 death receptor by selective internalization and degradation of DR4.

    Science.gov (United States)

    Bychkov, Maxim L; Gasparian, Marine E; Dolgikh, Dmitry A; Kirpichnikov, Mikhail P

    2014-01-01

    TRAIL (tumor necrosis factor-related apoptosis-inducing ligand) mediates apoptosis in cancer cells through death receptors DR4 and DR5 preferring often one receptor over another in the cells expressing both receptors. Receptor selective mutant variants of TRAIL and agonistic antibodies against DR4 and DR5 are highly promising anticancer agents. Here using DR5 specific mutant variant of TRAIL--DR5-B we have demonstrated for the first time that the sensitivity of cancer cells can be shifted from one TRAIL death receptor to another during co-treatment with anticancer drugs. First we have studied the contribution of DR4 and DR5 in HCT116 p53+/+ and HCT116 p53-/- cells and demonstrated that in HCT116 p53+/+ cells the both death receptors are involved in TRAIL-induced cell death while in HCT116 p53-/- cells prevailed DR4 signaling. The expression of death (DR4 and DR5) as well as decoy (DcR1 and DcR2) receptors was upregulated in the both cell lines either by TRAIL or by bortezomib. However, combined treatment of cells with two drugs induced strong time-dependent and p53-independent internalization and further lysosomal degradation of DR4 receptor. Interestingly DR5-B variant of TRAIL which do not bind with DR4 receptor also induced elimination of DR4 from cell surface in combination with bortezomib indicating the ligand-independent mechanism of the receptor internalization. Eliminatory internalization of DR4 resulted in activation of DR5 receptor thus DR4-dependent HCT116 p53-/- cells became highly sensitive to DR5-B in time-dependent manner. Internalization and degradation of DR4 receptor depended on activation of caspases as well as of lysosomal activity as it was completely inhibited by Z-VAD-FMK, E-64 and Baf-A1. In light of our findings, it is important to explore carefully which of the death receptors is active, when sensitizing drugs are combined with agonistic antibodies to the death receptors or receptor selective variants of TRAIL to enhance cancer treatment

  19. Psychological experiment

    NARCIS (Netherlands)

    Boven, Martijn; Emmanuel, Steven M.; McDonald, William; Stewart, Jon

    2015-01-01

    For Kierkegaard the ‘psychological experiment’ is a literary strategy. It enables him to dramatize an existential conflict in an experimental mode. Kierkegaard’s aim is to study the source of movement that animates the existing individual (this is the psychological part). However, he is not interest

  20. Rational Emotive Behavioural Therapy: The Evolution of a Revolution: Interview With Dr. Debbie Joffe Ellis, Work Partner and Wife of Dr. Albert Ellis, the Creator of REBT.

    Science.gov (United States)

    Ellis, Debbie Joffe; Rovira, Montse

    2015-02-01

    Recognized as one of the most influential thinkers and psychologists, Albert Ellis PhD (1913-2007) revolutionized Psychology when he created the first cognitive psychotherapy, Rational Emotive Behavioral Therapy. After he passed away, Dr. Debbie Joffe Ellis continues spreading his legacy around the world. Psychologist, lecturer, writer, trainer, she dedicates her life to disseminate REBT and extend it through different statements, from the social to the educational, from the academic to the clinical. In this interview, she goes through her own history and her husband's one, bringing us closer to understanding Albert Ellis as the leading figure in his field, and the oneness they experienced through their professional and personal relationship.

  1. Dr. von Braun and Dr. Stuhlinger With a Model of the Nuclear-Electric Vehicles

    Science.gov (United States)

    2004-01-01

    In this photo, taken at the Walt Disney Studios in California, Dr. Wernher von Braun and Dr. Ernst Stuhlinger are shown discussing the concepts of nuclear-electric spaceships designed to undertake the mission to the planet Mars. As a part of the Disney 'Tomorrowland' series on the exploration of space, the nuclear-electric vehicles were shown in the last three television films, entitled 'Mars and Beyond,' which first aired in December 1957.

  2. CRISPR/Cas9-mediated gene editing in human zygotes using Cas9 protein.

    Science.gov (United States)

    Tang, Lichun; Zeng, Yanting; Du, Hongzi; Gong, Mengmeng; Peng, Jin; Zhang, Buxi; Lei, Ming; Zhao, Fang; Wang, Weihua; Li, Xiaowei; Liu, Jianqiao

    2017-03-01

    Previous works using human tripronuclear zygotes suggested that the clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 system could be a tool in correcting disease-causing mutations. However, whether this system was applicable in normal human (dual pronuclear, 2PN) zygotes was unclear. Here we demonstrate that CRISPR/Cas9 is also effective as a gene-editing tool in human 2PN zygotes. By injection of Cas9 protein complexed with the appropriate sgRNAs and homology donors into one-cell human embryos, we demonstrated efficient homologous recombination-mediated correction of point mutations in HBB and G6PD. However, our results also reveal limitations of this correction procedure and highlight the need for further research.

  3. CRISPR-Cas adaptive immune systems of the sulfolobales

    DEFF Research Database (Denmark)

    Garrett, Roger Antony; Shah, Shiraz Ali; Erdmann, Susanne;

    2015-01-01

    The Sulfolobales have provided good model organisms for studying CRISPR-Cas systems of the crenarchaeal kingdom of the archaea. These organisms are infected by a wide range of exceptional archaea-specific viruses and conjugative plasmids, and their CRISPR-Cas systems generally exhibit extensive......, and this is consistent with these proteins tending to coevolve with core Cas proteins. Various novel aspects of CRISPR-Cas systems of the Sulfolobales are considered including an alternative spacer acquisition mechanism, reversible spacer acquisition, the formation and significance of antisense CRISPR RNAs, and a novel...... mechanism for avoidance of CRISPR-Cas defense. Finally, questions regarding the basis for the complexity, diversity, and apparent redundancy, of the intracellular CRISPR-Cas systems are discussed....

  4. CRISPR-Cas9 Based Engineering of Actinomycetal Genomes

    DEFF Research Database (Denmark)

    Tong, Yaojun; Charusanti, Pep; Zhang, Lixin

    2015-01-01

    . To facilitate the genetic manipulation of actinomycetes, we developed a highly efficient CRISPR-Cas9 system to delete gene(s) or gene cluster(s), implement precise gene replacements, and reversibly control gene expression in actinomycetes. We demonstrate our system by targeting two genes, actIORF1 (SCO5087......) and actVB (SCO5092), from the actinorhodin biosynthetic gene cluster in Streptomyces coelicolor A3(2). Our CRISPR-Cas9 system successfully inactivated the targeted genes. When no templates for homology-directed repair (HDR) were present, the site-specific DNA double-strand breaks (DSBs) introduced by Cas9....... Moreover, we developed a system to efficiently and reversibly control expression of target genes, deemed CRISPRi, based on a catalytically dead variant of Cas9 (dCas9). The CRISPR-Cas9 based system described here comprises a powerful and broadly applicable set of tools to manipulate actinomycetal genomes....

  5. CRISPR/Cas9: Transcending the Reality of Genome Editing

    Directory of Open Access Journals (Sweden)

    Sergiu Chira

    2017-06-01

    Full Text Available With the expansion of the microbiology field of research, a new genome editing tool arises from the biology of bacteria that holds the promise of achieving precise modifications in the genome with a simplicity and versatility that surpasses previous genome editing methods. This new technique, commonly named CRISPR/Cas9, led to a rapid expansion of the biomedical field; more specifically, cancer characterization and modeling have benefitted greatly from the genome editing capabilities of CRISPR/Cas9. In this paper, we briefly summarize recent improvements in CRISPR/Cas9 design meant to overcome the limitations that have arisen from the nuclease activity of Cas9 and the influence of this technology in cancer research. In addition, we present challenges that might impede the clinical applicability of CRISPR/Cas9 for cancer therapy and highlight future directions for designing CRISPR/Cas9 delivery systems that might prove useful for cancer therapeutics.

  6. Advances in therapeutic CRISPR/Cas9 genome editing.

    Science.gov (United States)

    Savić, Nataša; Schwank, Gerald

    2016-02-01

    Targeted nucleases are widely used as tools for genome editing. Two years ago the clustered regularly interspaced short palindromic repeat (CRISPR)-associated Cas9 nuclease was used for the first time, and since then has largely revolutionized the field. The tremendous success of the CRISPR/Cas9 genome editing tool is powered by the ease design principle of the guide RNA that targets Cas9 to the desired DNA locus, and by the high specificity and efficiency of CRISPR/Cas9-generated DNA breaks. Several studies recently used CRISPR/Cas9 to successfully modulate disease-causing alleles in vivo in animal models and ex vivo in somatic and induced pluripotent stem cells, raising hope for therapeutic genome editing in the clinics. In this review, we will summarize and discuss such preclinical CRISPR/Cas9 gene therapy reports. Copyright © 2016 Elsevier Inc. All rights reserved.

  7. [Criminal psychology in Franco's police].

    Science.gov (United States)

    Bandrés, Javier; Llavona, Rafael; Zubieta, Eva

    2013-02-01

    Francisco J. de Echalecu (1897-1957) was a Spanish psychiatrist who held important positions, such as Psychology Professor at the Academia General de Policía and Neuropsychiatrist at the Dirección General de Seguridad. This work provides a brief biography of Echalecu and analyzes the transcriptions of his classes on Criminal Psychology of 1942, his Criminal Psychology from 1947 as well as his involvement in the case of the torture of Communist leader Heriberto Quiñones. We describe his project of a totalitarian Psychology and his proposal of social intervention, including eugenic methodologies as well as forced reclusion for those labeled as asocial. The adaptation in Spain of the totalitarian psychological project to the new international reality after the Second World War is also described. In Spain a "final solution" for criminals and political dissidents has been prepared, which was inspired by the Nazi criminal policies and promoted by Dr. Echalecu from Spain's higher police body, the DGS. This project was frustrated by the German defeat in the world war and the only thing left from the original project was the arbitrary application of the Ley de Vagos y Maleantes [an antivagrancy law] to those individuals labeled as "asocial".

  8. Coaching and engaging. Developing teaching with CAS in High School

    DEFF Research Database (Denmark)

    Bang, Henrik Peter; Grønbæk, Niels; Larsen, Claus Richard

    The extensive use of CAS at upper secondary school in Denmark provides a laboratory for research on the development of standards for CAS teaching. The poster focuses on action research into teachers developing lessons and student activities in an ongoing collaboration between university and high...... schools on use of CAS in mathematics teaching. Coaches1 mediate design processes, reflection and documentation, and enable sharing. We discuss coaching as a valuable part of action research, and how to draw findings from the collaboration....

  9. CAS Announces Its Action Plan for Revitalizing Northeast China

    Institute of Scientific and Technical Information of China (English)

    2004-01-01

    @@ A conference of the CAS leading group for the revitalization of northeast China with science and technology was held on Sept. 1 and 2 in Changchun, capital of northeast China's Jilin Province. Chaired by CAS Vice President Shi Erwei, the meeting focused on the implementation of a CAS action plan for retrieving China's northeast, an old industrial base and once the industrialization locomotive of the country.

  10. Decommissioning of DR 1, Final report

    Energy Technology Data Exchange (ETDEWEB)

    Lauridsen, Kurt

    2006-01-15

    The report describes the decommissioning activities carried out at the 2kW homogeneous reactor DR 1 at Risoe National Laboratory. The decommissioning work took place from summer 2004 until late autumn 2005. The components with the highest activity, the core vessel the recombiner and the piping and valves connected to these, were dismantled first by Danish Decommissioning's own technicians. Demolition of the control rod house and the biological shield as well as the removal of the floor in the reactor hall was carried out by an external demolition contractor. The building was emptied and left for other use. Clearance measurements of the building showed that radionuclide concentrations were everywhere below the clearance limit set by the Danish nuclear regulatory authorities. Furthermore, measurements on the surrounding area showed that there was no contamination that could be attributed to the operation and decommissioning of DR 1. (au)

  11. [Homage to Professor Dr. Nicasio Etchepareborda].

    Science.gov (United States)

    1998-11-01

    During a solemn academic act, de Main Classroom of the Facultad de Odontologia de Buenos Aires was named after Prof. Dr. Nicasio Etchepareborda. He has been the first professor at the Escuela de Odontologia and its organizer, after having obtained his Dentistry degree at the Dental School of Paris, in 1882. The new school was founded in 1891, and its activities began the following year.

  12. This Is Not Dr. Conn's Aldosterone Anymore

    OpenAIRE

    Brown, Nancy J.

    2011-01-01

    In 1955, Dr. Jerome Conn described a patient with severe hypertension and hypokalemia and an aldosterone-secreting adenoma. The prevalence of hyperaldosteronism is increased among patients with obesity or resistant hypertension. Angiotensin-converting enzyme (ACE) inhibitors and angiotensin receptor blockers reduce the secretion of aldosterone, but with chronic treatment aldosterone concentrations “escape” back to baseline values. Mineralocorticoid receptor (MR) antagonism reduces mortality i...

  13. DR21 Main: A Collapsing Cloud

    OpenAIRE

    Kirby, Larry

    2008-01-01

    The molecular cloud, DR21 Main, is an example of a large-scale gravitational collapse about an axis near the plane of the sky where the collapse is free of major disturbances due to rotation or other effects. Using flux maps, polarimetric maps, and measurements of the field inclination by comparing the line widths of ion and neutral species, we estimate the temperature, mass, magnetic field, and the turbulent kinetic, mean magnetic, and gravitational potential energies, and present a 3D model...

  14. Ectopia cordis thoracique sporadique: description clinique d'un cas

    Science.gov (United States)

    Lubala, Toni Kasole; Mutombo, Augustin Mulangu; Katamea, Tina; Lubala, Nina; Munkana, Arthur Ndundula; Kabuya, Maguy Sangaji; Monga, Joséphine Kalenga; Luboya, Oscar Numbi

    2012-01-01

    Nous décrivons un cas d'ectopia cordis, une malformation cardiaque congénitale extrêmement rare dans laquelle le coeur est partiellement ou complètement situé en dehors des limites de la cage thoracique. Dans le cas que nous décrivons, elle est thoracique et isolée. Ce cas a été diagnostiqué en salle de naissance au Katanga, au sud de la République Démocratique du Congo. Il s'agit du premier cas documenté chez un nouveau-né Congolais. PMID:23346276

  15. CRISPR-Cas9-guided Genome Engineering in C. elegans

    Science.gov (United States)

    Kim, Hyun-Min; Colaiácovo, Monica P.

    2016-01-01

    The CRISPR (clustered regularly interspaced short palindromic repeats)-Cas (CRISPR-associated) system is successfully being used for efficient and targeted genome editing in various organisms including the nematode C. elegans. Recent studies developed various CRISPR-Cas9 approaches to enhance genome engineering via two major DNA double-strand break repair pathways: non-homologous end joining and homologous recombination. Here we describe a protocol for Cas9-mediated C. elegans genome editing together with single guide RNA (sgRNA) and repair template cloning and injection methods required for delivering Cas9, sgRNAs and repair template DNA into the C. elegans germline. PMID:27366893

  16. Control of gene expression by CRISPR-Cas systems

    Science.gov (United States)

    2013-01-01

    Clustered regularly interspaced short palindromic repeats (CRISPR) loci and their associated cas (CRISPR-associated) genes provide adaptive immunity against viruses (phages) and other mobile genetic elements in bacteria and archaea. While most of the early work has largely been dominated by examples of CRISPR-Cas systems directing the cleavage of phage or plasmid DNA, recent studies have revealed a more complex landscape where CRISPR-Cas loci might be involved in gene regulation. In this review, we summarize the role of these loci in the regulation of gene expression as well as the recent development of synthetic gene regulation using engineered CRISPR-Cas systems. PMID:24273648

  17. The 6th Meeting of CAS Board of Executive Directors

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    @@ The 6th meeting of the board of executive directors of China Association for Standardization (CAS) (the 2nd chairman meeting in 2005) was held in Beijing on January 19th, 2006. The attendees were Mr. Li Zhonghai, President of CAS, who presided over the meeting, Ms. Li Rui and Mr. Li Chuntian, Advisers of CAS, Sun Xiaokang and Shi Baoquan, Vice Directors of SAC (Standardization Administration of China), Jiao Yunqi, President of CNIS (China National Institute of Standardization), Zhang Jianquan, Director of SPC (the Standards Press of China), over 20executive directors and some CAS officers.

  18. Gaia DR1 documentation Chapter 6: Variability

    Science.gov (United States)

    Eyer, L.; Rimoldini, L.; Guy, L.; Holl, B.; Clementini, G.; Cuypers, J.; Mowlavi, N.; Lecoeur-Taïbi, I.; De Ridder, J.; Charnas, J.; Nienartowicz, K.

    2017-02-01

    This chapter describes the photometric variability processing of the Gaia DR1 data. Coordination Unit 7 is responsible for the variability analysis of over a billion celestial sources. In particular the definition, design, development, validation and provision of a software package for the data processing of photometrically variable objects. Data Processing Centre Geneva (DPCG) responsibilities cover all issues related to the computational part of the CU7 analysis. These span: hardware provisioning, including selection, deployment and optimisation of suitable hardware, choosing and developing software architecture, defining data and scientific workflows as well as operational activities such as configuration management, data import, time series reconstruction, storage and processing handling, visualisation and data export. CU7/DPCG is also responsible for interaction with other DPCs and CUs, software and programming training for the CU7 members, scientific software quality control and management of software and data lifecycle. Details about the specific data treatment steps of the Gaia DR1 data products are found in Eyer et al. (2017) and are not repeated here. The variability content of the Gaia DR1 focusses on a subsample of Cepheids and RR Lyrae stars around the South ecliptic pole, showcasing the performance of the Gaia photometry with respect to variable objects.

  19. DR-Cloud: Multi-Cloud Based Disaster Recovery Service

    National Research Council Canada - National Science Library

    Yu Gu DongshengWang Ghuanyi Liu

    2014-01-01

    .... This paper presents a practical multi-cloud based disaster recovery service model: DR- Cloud. With DR-Cloud, resources of multiple cloud service providers can be utilized cooperatively by the disaster recovery service provider...

  20. Dr. William C. Harris, Director-General, Science Foundation Ireland

    CERN Multimedia

    Maximilien Brice

    2002-01-01

    Photo 01: Dr William C. Harris, Director-General, Science Foundation Ireland (left) with R. Cashmore. Photos 02, 03: Dr William C. Harris, Director-General, Science Foundation Ireland signing the CERN guest book with R. Cashmore.

  1. Dr. Yu Wang, Director, Natural Science Division, National Science Council, Taiwan

    CERN Multimedia

    Patrice Loïez

    2001-01-01

    Photo 01: L. to r.: Dr. Philippe Bloch, CERN CMS ECAL Deputy Project Manager, Dr. Yu Wang, Dr. Etiennette Auffray, CERN, responsible of the CERN ECAL Regional Centre. Photo 02: L. to r.: Dr. Yu Wang, Dr. Philippe Bloch, Dr. Apollo GO, National Central University, Taiwan, Dr. Etiennette Auffray.

  2. Le tourisme noir : l’étrange cas du Dr Jekyll et de M. Hyde

    OpenAIRE

    BAILLARGEON, Taïka

    2016-01-01

    Depuis quelques années déjà, de nombreux chercheurs et autres experts du tourisme s’intéressent à un intriguant phénomène que Malcom Foley et J. John Lennon (1996) ont nommé le « tourisme noir » (dark tourism). Philip R. Stone (2006), directeur de l’Institute for Dark Tourism Research, définit cette pratique comme « the act of travel to sites associated with death, suffering and the seemingly macabre » (2006 : 146). Cette pratique est généralement présentée comme un phénomène récent : comme s...

  3. New CRISPR–Cas systems from uncultivated microbes

    Science.gov (United States)

    Burstein, David; Harrington, Lucas B.; Strutt, Steven C.; Probst, Alexander J.; Anantharaman, Karthik; Thomas, Brian C.; Doudna, Jennifer A.; Banfield, Jillian F.

    2016-12-01

    CRISPR–Cas systems provide microbes with adaptive immunity by employing short DNA sequences, termed spacers, that guide Cas proteins to cleave foreign DNA. Class 2 CRISPR–Cas systems are streamlined versions, in which a single RNA-bound Cas protein recognizes and cleaves target sequences. The programmable nature of these minimal systems has enabled researchers to repurpose them into a versatile technology that is broadly revolutionizing biological and clinical research. However, current CRISPR–Cas technologies are based solely on systems from isolated bacteria, leaving the vast majority of enzymes from organisms that have not been cultured untapped. Metagenomics, the sequencing of DNA extracted directly from natural microbial communities, provides access to the genetic material of a huge array of uncultivated organisms. Here, using genome-resolved metagenomics, we identify a number of CRISPR–Cas systems, including the first reported Cas9 in the archaeal domain of life, to our knowledge. This divergent Cas9 protein was found in little-studied nanoarchaea as part of an active CRISPR–Cas system. In bacteria, we discovered two previously unknown systems, CRISPR–CasX and CRISPR–CasY, which are among the most compact systems yet discovered. Notably, all required functional components were identified by metagenomics, enabling validation of robust in vivo RNA-guided DNA interference activity in Escherichia coli. Interrogation of environmental microbial communities combined with in vivo experiments allows us to access an unprecedented diversity of genomes, the content of which will expand the repertoire of microbe-based biotechnologies.

  4. Recent Advances in Genome Editing Using CRISPR/Cas9.

    Science.gov (United States)

    Ding, Yuduan; Li, Hong; Chen, Ling-Ling; Xie, Kabin

    2016-01-01

    The CRISPR (clustered regularly interspaced short palindromic repeat)-Cas9 (CRISPR-associated nuclease 9) system is a versatile tool for genome engineering that uses a guide RNA (gRNA) to target Cas9 to a specific sequence. This simple RNA-guided genome-editing technology has become a revolutionary tool in biology and has many innovative applications in different fields. In this review, we briefly introduce the Cas9-mediated genome-editing method, summarize the recent advances in CRISPR/Cas9 technology, and discuss their implications for plant research. To date, targeted gene knockout using the Cas9/gRNA system has been established in many plant species, and the targeting efficiency and capacity of Cas9 has been improved by optimizing its expression and that of its gRNA. The CRISPR/Cas9 system can also be used for sequence-specific mutagenesis/integration and transcriptional control of target genes. We also discuss off-target effects and the constraint that the protospacer-adjacent motif (PAM) puts on CRISPR/Cas9 genome engineering. To address these problems, a number of bioinformatic tools are available to help design specific gRNAs, and new Cas9 variants and orthologs with high fidelity and alternative PAM specificities have been engineered. Owing to these recent efforts, the CRISPR/Cas9 system is becoming a revolutionary and flexible tool for genome engineering. Adoption of the CRISPR/Cas9 technology in plant research would enable the investigation of plant biology at an unprecedented depth and create innovative applications in precise crop breeding.

  5. Recent Advances in Genome Editing Using CRISPR/Cas9

    Directory of Open Access Journals (Sweden)

    Yuduan eDing

    2016-05-01

    Full Text Available AbstractThe CRISPR (clustered regularly interspaced short palindromic repeat-Cas9 (CRISPR-associated nuclease 9 system is a versatile tool for genome engineering that uses a guide RNA (gRNA to target Cas9 to a specific sequence. This simple RNA-guided genome-editing technology has become a revolutionary tool in biology and has many innovative applications in different fields. In this review, we briefly introduce the Cas9-mediated genome-editing method, summarize the recent advances in CRISPR/Cas9 technology, and discuss their implications for plant research. To date, targeted gene knockout using the Cas9/gRNA system has been established in many plant species, and the targeting efficiency and capacity of Cas9 has been improved by optimizing its expression and that of its gRNA. The CRISPR/Cas9 system can also be used for sequence-specific mutagenesis/integration and transcriptional control of target genes. We also discuss off-target effects and the constraint that the protospacer-adjacent motif (PAM puts on CRISPR/Cas9 genome engineering. To address these problems, a number of bioinformatic tools are available to help design specific gRNAs, and new Cas9 variants and orthologs with high fidelity and alternative PAM specificities have been engineered. Owing to these recent efforts, the CRISPR/Cas9 system is becoming a revolutionary and flexible tool for genome engineering. Adoption of the CRISPR/Cas9 technology in plant research would enable the investigation of plant biology at an unprecedented depth and create innovative applications in precise crop breeding.

  6. New CRISPR-Cas systems from uncultivated microbes

    Science.gov (United States)

    Burstein, David; Harrington, Lucas B.; Strutt, Steven C.; Probst, Alexander J.; Anantharaman, Karthik; Thomas, Brian C.; Doudna, Jennifer A.; Banfield, Jillian F.

    2017-02-01

    CRISPR-Cas systems provide microbes with adaptive immunity by employing short DNA sequences, termed spacers, that guide Cas proteins to cleave foreign DNA. Class 2 CRISPR-Cas systems are streamlined versions, in which a single RNA-bound Cas protein recognizes and cleaves target sequences. The programmable nature of these minimal systems has enabled researchers to repurpose them into a versatile technology that is broadly revolutionizing biological and clinical research. However, current CRISPR-Cas technologies are based solely on systems from isolated bacteria, leaving the vast majority of enzymes from organisms that have not been cultured untapped. Metagenomics, the sequencing of DNA extracted directly from natural microbial communities, provides access to the genetic material of a huge array of uncultivated organisms. Here, using genome-resolved metagenomics, we identify a number of CRISPR-Cas systems, including the first reported Cas9 in the archaeal domain of life, to our knowledge. This divergent Cas9 protein was found in little-studied nanoarchaea as part of an active CRISPR-Cas system. In bacteria, we discovered two previously unknown systems, CRISPR-CasX and CRISPR-CasY, which are among the most compact systems yet discovered. Notably, all required functional components were identified by metagenomics, enabling validation of robust in vivo RNA-guided DNA interference activity in Escherichia coli. Interrogation of environmental microbial communities combined with in vivo experiments allows us to access an unprecedented diversity of genomes, the content of which will expand the repertoire of microbe-based biotechnologies.

  7. Dr. David Syz, State Secretary for Economic Affairs, Switzerland

    CERN Multimedia

    Maximilien Brice

    2003-01-01

    Dr. David Syz, State Secretary for Economic Affairs, Switzerland, toured the assembly hall of the ATLAS experiment on a recent visit to CERN.Photos 01, 02: Dr. Peter Jenni, spokesperson for the ATLAS experiment (second from left), explains to Dr. David Syz (fourth from left) and accompanying visitors the process of integration of a 26-metre-long coil of the barrel toroid magnet system into its coil casing.Photo 03: Dr. Peter Jenni (extreme right) with Dr. David Syz (front row, fourth from right) behind a stack of 26-metre-long 'racetrack' coils awaiting integration into their coil casings.

  8. CRISPR/Cas9 based genome editing of Penicillium chrysogenum

    NARCIS (Netherlands)

    Pohl, Carsten; Kiel, Jan A K W; Driessen, Arnold J M; Bovenberg, Roel A L; Nygård, Yvonne

    2016-01-01

    CRISPR/Cas9 based systems have emerged as versatile platforms for precision genome editing in a wide range of organisms. Here we have developed powerful CRISPR/Cas9 tools for marker-based and marker-free genome modifications in Penicillium chrysogenum, a model filamentous fungus and industrially rel

  9. CAS Scientists Find New Anti-SARS Compounds

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    @@ Scientists from the CAS Shanghai Institute of Materia Medica (SIMM) and the National Center for Drug Screening (NCDS) have identified several novel compounds that could be potential weapons to combat the SARS epidemic. This was announced at a news briefing held by the CAS Shanghai Institutes for Biological Sciences on June 19 in Shanghai.

  10. First International Student Obtains His PhD at CAS

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    @@ Mr. Ghulam Rasul, the first international student formally enrolled by the Graduate University of CAS (GUCAS) has received his PhD degree this summer after having passed his dissertation defense on May 21 at the CAS Institute of Atmospheric Physics (IAP).

  11. CAS issues its 2007 prizes for outstanding achievements

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    @@ One individual and three research groups have been honored with the prestigious CAS Awards for Outstanding S&T Achievements in 2007. CAS President LU Yongxiang conferred the awards on the laureates at the Academy's annual work conference opened on 24 March in Beijing.

  12. CAS, Shanghai, Pasteur to Jointly Set Up a New Institute

    Institute of Scientific and Technical Information of China (English)

    2004-01-01

    @@ An agreement for jointly establishing the CAS Institut Pasteur of Shanghai was signed on August 30 in east China's Shanghai Municipality. Representatives from the collaborative partners, including Shanghai Vice Mayor Yan Junqi, CAS Vice President Chen Zhu and President of Institut Pasteur Philippe Kourilsky witnessed the signing ceremony.

  13. Reaction to Indispensable Manual Calculation Skills in a CAS Environment.

    Science.gov (United States)

    Monaghan, John

    2001-01-01

    Reacts to an article published in a previous issue of this journal on the effects of graphing calculators and computer algebra systems (CAS) on students' manual calculation and algebraic manipulation skills. Considers the contribution made by Jean-Baptiste Lagrange to thinking about the role of CAS in teaching algebra. (ASK)

  14. CAS conducts ex-post evaluation on research projects

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    @@ Under the auspices of the CAS Center for Project Supervision and Evaluation, a panel of experts recently conducted an ex-post evaluation on a research project entitled "the electrical system for an electric vehicle." The appraisal is the first of its kind in project management at CAS.

  15. Recent Progress in CRISPR/Cas9 Technology.

    Science.gov (United States)

    Mei, Yue; Wang, Yan; Chen, Huiqian; Sun, Zhong Sheng; Ju, Xing-Da

    2016-02-20

    The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system, a simple and efficient tool for genome editing, has experienced rapid progress in its technology and applicability in the past two years. Here, we review the recent advances in CRISPR/Cas9 technology and the ways that have been adopted to expand our capacity for precise genome manipulation. First, we introduce the mechanism of CRISPR/Cas9, including its biochemical and structural implications. Second, we highlight the latest improvements in the CRISPR/Cas9 system, especially Cas9 protein modifications for customization. Third, we review its current applications, in which the versatile CRISPR/Cas9 system was employed to edit the genome, epigenome, or RNA of various organisms. Although CRISPR/Cas9 allows convenient genome editing accompanied by many benefits, we should not ignore the significant ethical and biosafety concerns that it raises. Finally, we discuss the prospective applications and challenges of several promising techniques adapted from CRISPR/Cas9.

  16. Progress Scored in Management Information System at CAS

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    @@ CAS initiative to upgrade its management information system (MIS) is making significant progress. Recently, 116 CAS subordinates have completed their online trial operation of a MIS project at the Academy, called Academia Resource Planning (ARP), marking an important phased achievement of the initiative.

  17. Teaching Undergraduate Mathematics Using CAS Technology: Issues and Prospects

    Science.gov (United States)

    Tobin, Patrick C.; Weiss, Vida

    2016-01-01

    The use of handheld CAS technology in undergraduate mathematics courses in Australia is paradoxically shrinking under sustained disapproval or disdain from the professional mathematics community. Mathematics education specialists argue with their mathematics colleagues over a range of issues in course development and this use of CAS or even…

  18. Transformation of OODT CAS to Perform Larger Tasks

    Science.gov (United States)

    Mattmann, Chris; Freeborn, Dana; Crichton, Daniel; Hughes, John; Ramirez, Paul; Hardman, Sean; Woollard, David; Kelly, Sean

    2008-01-01

    A computer program denoted OODT CAS has been transformed to enable performance of larger tasks that involve greatly increased data volumes and increasingly intensive processing of data on heterogeneous, geographically dispersed computers. Prior to the transformation, OODT CAS (also alternatively denoted, simply, 'CAS') [wherein 'OODT' signifies 'Object-Oriented Data Technology' and 'CAS' signifies 'Catalog and Archive Service'] was a proven software component used to manage scientific data from spaceflight missions. In the transformation, CAS was split into two separate components representing its canonical capabilities: file management and workflow management. In addition, CAS was augmented by addition of a resource-management component. This third component enables CAS to manage heterogeneous computing by use of diverse resources, including high-performance clusters of computers, commodity computing hardware, and grid computing infrastructures. CAS is now more easily maintainable, evolvable, and reusable. These components can be used separately or, taking advantage of synergies, can be used together. Other elements of the transformation included addition of a separate Web presentation layer that supports distribution of data products via Really Simple Syndication (RSS) feeds, and provision for full Resource Description Framework (RDF) exports of metadata.

  19. Optimization of genome editing through CRISPR-Cas9 engineering.

    Science.gov (United States)

    Zhang, Jian-Hua; Adikaram, Poorni; Pandey, Mritunjay; Genis, Allison; Simonds, William F

    2016-04-01

    CRISPR (Clustered Regularly-Interspaced Short Palindromic Repeats)-Cas9 (CRISPR associated protein 9) has rapidly become the most promising genome editing tool with great potential to revolutionize medicine. Through guidance of a 20 nucleotide RNA (gRNA), CRISPR-Cas9 finds and cuts target protospacer DNA precisely 3 base pairs upstream of a PAM (Protospacer Adjacent Motif). The broken DNA ends are repaired by either NHEJ (Non-Homologous End Joining) resulting in small indels, or by HDR (Homology Directed Repair) for precise gene or nucleotide replacement. Theoretically, CRISPR-Cas9 could be used to modify any genomic sequences, thereby providing a simple, easy, and cost effective means of genome wide gene editing. However, the off-target activity of CRISPR-Cas9 that cuts DNA sites with imperfect matches with gRNA have been of significant concern because clinical applications require 100% accuracy. Additionally, CRISPR-Cas9 has unpredictable efficiency among different DNA target sites and the PAM requirements greatly restrict its genome editing frequency. A large number of efforts have been made to address these impeding issues, but much more is needed to fully realize the medical potential of CRISPR-Cas9. In this article, we summarize the existing problems and current advances of the CRISPR-Cas9 technology and provide perspectives for the ultimate perfection of Cas9-mediated genome editing.

  20. CRISPR-Cas9 Genome Engineering in Saccharomyces cerevisiae Cells.

    Science.gov (United States)

    Ryan, Owen W; Poddar, Snigdha; Cate, Jamie H D

    2016-06-01

    This protocol describes a method for CRISPR-Cas9-mediated genome editing that results in scarless and marker-free integrations of DNA into Saccharomyces cerevisiae genomes. DNA integration results from cotransforming (1) a single plasmid (pCAS) that coexpresses the Cas9 endonuclease and a uniquely engineered single guide RNA (sgRNA) expression cassette and (2) a linear DNA molecule that is used to repair the chromosomal DNA damage by homology-directed repair. For target specificity, the pCAS plasmid requires only a single cloning modification: replacing the 20-bp guide RNA sequence within the sgRNA cassette. This CRISPR-Cas9 protocol includes methods for (1) cloning the unique target sequence into pCAS, (2) assembly of the double-stranded DNA repair oligonucleotides, and (3) cotransformation of pCAS and linear repair DNA into yeast cells. The protocol is technically facile and requires no special equipment. It can be used in any S. cerevisiae strain, including industrial polyploid isolates. Therefore, this CRISPR-Cas9-based DNA integration protocol is achievable by virtually any yeast genetics and molecular biology laboratory.

  1. Engineering the Caenorhabditis elegans genome with CRISPR/Cas9

    NARCIS (Netherlands)

    Waaijers, Selma; Boxem, Mike

    2014-01-01

    The development in early 2013 of CRISPR/Cas9-based genome engineering promises to dramatically advance our ability to alter the genomes of model systems at will. A single, easily produced targeting RNA guides the Cas9 endonuclease to a specific DNA sequence where it creates a double strand break. Im

  2. Prof. DR. F. C. Eloff - An appreciation

    Directory of Open Access Journals (Sweden)

    G de Graaff

    1984-12-01

    Full Text Available I have been requested to write an appreciation of the man to whom these proceedings of a symposium on the Kalahari Ecosystem are dedicated @ Prof. Dr. F. C. Eloff, or Fritz as he is popularly referred to. I undertook the task with some trepidation and the only claim to the honour to write this article may be the fact that I have known Professor Eloff since 1949 when I was a green-shanked first year veterinary student at the University of Pretoria where he lectured in zoology to the new students.

  3. An Interview with Dr.Joseph Hung

    Institute of Scientific and Technical Information of China (English)

    包天仁

    2007-01-01

    @@ Prof.Bao Tianren(Bao):Dr.Hung,thank you for joining us in the 2nd National English as a Foreign Language Teaching Conference for Primary and Secondary Teachers of English in Guangzhou.We organize this conference every year and the theme of this year is Approaches on Raising China's English Language Teaching and Learning Efficiency.The current ELT situation for primary and secondary students in China is rather ineffective.Students learn English for 3 years or more in primary schools,but when they upgrade in junior schools,most of them will have to study English from the very beginning again.

  4. Achieving a milestone: AMOR is now archived in Portico and indexed in CAS

    Directory of Open Access Journals (Sweden)

    R.N Sugitha Nadarajah

    2016-10-01

    Full Text Available The journal of Advances in Modern Oncology Research (AMOR is proud to announce its partnership with Portico, a leading digital preservation service provider, to ensure the long term accessibility of its published contents. In addition, it is now indexed by the gold standard of chemical information database Chemical Abstracts Services (CAS after publishing only six bimonthly issues, dating back to October 2015. AMOR’s Editor-in-Chief Dr. Omar Abdel-Rahman proudly shares: “Our vision is to be far more than just a journal, we want to be a platform that publishes high quality cancer research contents from all over the world. I think we are progressing, at an acceptable pace, in that direction."The Portico archive is a “centralized repository of tens of thousands of e-journals, e-books, and other electronic content, replicated to ensure security,” according to the registry organization. “Content comes into the archive under formal preservation agreements with publishers. Content providers submit source files to Portico, and we repackage these source files into an archival format and provide long-term archival management and format migration as needed. Our approach is driven by our commitment to meeting clear preservation goals,” it states. AMOR’s Managing Editor Dr. TS Jong, when asked to comment about the impact of this development, adds: “AMOR is committed towards meeting the highest international publication standards and as such, has a clear archiving and indexing roadmap to sustain our growth. The journal acknowledges the importance of ensuring the continuous accessibility of our published articles in multiple repositories. Therefore, we are delighted with the recent partnership between AMOR and Portico, a well-known third-party repository service provider, to safeguard the long-term availability of our contents.”Archiving AMOR’s articles within Portico ensures that the materials published are always available for access. In

  5. Psychological IVF

    DEFF Research Database (Denmark)

    Adrian, Stine Willum

    2015-01-01

    During ethnographic fieldwork at a fertility clinic in Denmark, I became intrigued by emotions. In particular, I found an incidence labelled ‘psychological IVF’ theoretically provocative as it challenged my views on materializations, which I was preparing to study. This paper centres on the story...... of psychological IVF, and I use this narrative to consider emotions and materialization methodologically. I also ask how emotions at fertility clinics can be conceptualized to enable analysis of their materialization, change, and effects. In order to do so, I develop the term ‘emotional choreography......’. This theoretical work has three aims. First, it seeks to illustrate how the story of psychological IVF offers a rich range of materializations of emotions. Secondly, this work proposes a feminist materialist conceptualization of emotions that is both non-representational and posthuman. This conceptualization draws...

  6. EDUCATIONAL PSYCHOLOGY

    Directory of Open Access Journals (Sweden)

    Sunil Patil

    2009-06-01

    Full Text Available Education Psychology is one of the most rapidly advancing disciplines which is concerned with the description, explanation and control of the educational processes arising from the interaction between the teacher and learner in the context of a specified or unspecified subject. The concept of educational psycology is changing as a result of the general impact of the disciplines such as sociology, anthropology,economics, political science and so on. The subject matter of educational psychology is also reflecting a diversity of structures and emphases in its connection.

  7. Interference activity of a minimal Type I CRISPR-Cas system from Shewanella putrefaciens.

    Science.gov (United States)

    Dwarakanath, Srivatsa; Brenzinger, Susanne; Gleditzsch, Daniel; Plagens, André; Klingl, Andreas; Thormann, Kai; Randau, Lennart

    2015-10-15

    Type I CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)-Cas (CRISPR-associated) systems exist in bacterial and archaeal organisms and provide immunity against foreign DNA. The Cas protein content of the DNA interference complexes (termed Cascade) varies between different CRISPR-Cas subtypes. A minimal variant of the Type I-F system was identified in proteobacterial species including Shewanella putrefaciens CN-32. This variant lacks a large subunit (Csy1), Csy2 and Csy3 and contains two unclassified cas genes. The genome of S. putrefaciens CN-32 contains only five Cas proteins (Cas1, Cas3, Cas6f, Cas1821 and Cas1822) and a single CRISPR array with 81 spacers. RNA-Seq analyses revealed the transcription of this array and the maturation of crRNAs (CRISPR RNAs). Interference assays based on plasmid conjugation demonstrated that this CRISPR-Cas system is active in vivo and that activity is dependent on the recognition of the dinucleotide GG PAM (Protospacer Adjacent Motif) sequence and crRNA abundance. The deletion of cas1821 and cas1822 reduced the cellular crRNA pool. Recombinant Cas1821 was shown to form helical filaments bound to RNA molecules, which suggests its role as the Cascade backbone protein. A Cascade complex was isolated which contained multiple Cas1821 copies, Cas1822, Cas6f and mature crRNAs.

  8. Interference activity of a minimal Type I CRISPR–Cas system from Shewanella putrefaciens

    Science.gov (United States)

    Dwarakanath, Srivatsa; Brenzinger, Susanne; Gleditzsch, Daniel; Plagens, André; Klingl, Andreas; Thormann, Kai; Randau, Lennart

    2015-01-01

    Type I CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)–Cas (CRISPR-associated) systems exist in bacterial and archaeal organisms and provide immunity against foreign DNA. The Cas protein content of the DNA interference complexes (termed Cascade) varies between different CRISPR-Cas subtypes. A minimal variant of the Type I-F system was identified in proteobacterial species including Shewanella putrefaciens CN-32. This variant lacks a large subunit (Csy1), Csy2 and Csy3 and contains two unclassified cas genes. The genome of S. putrefaciens CN-32 contains only five Cas proteins (Cas1, Cas3, Cas6f, Cas1821 and Cas1822) and a single CRISPR array with 81 spacers. RNA-Seq analyses revealed the transcription of this array and the maturation of crRNAs (CRISPR RNAs). Interference assays based on plasmid conjugation demonstrated that this CRISPR-Cas system is active in vivo and that activity is dependent on the recognition of the dinucleotide GG PAM (Protospacer Adjacent Motif) sequence and crRNA abundance. The deletion of cas1821 and cas1822 reduced the cellular crRNA pool. Recombinant Cas1821 was shown to form helical filaments bound to RNA molecules, which suggests its role as the Cascade backbone protein. A Cascade complex was isolated which contained multiple Cas1821 copies, Cas1822, Cas6f and mature crRNAs. PMID:26350210

  9. Reality Therapy: Interviews with Dr. William Glasser.

    Science.gov (United States)

    Cockrum, J. Robert

    1989-01-01

    Provides background of Glasser's Reality Therapy (RT) since its inception in l960s. Discusses incorporation of Control Theory Psychology into Reality Therapy, growth of RT during past 16 years, and development of Educator Training Center. Cites l98l establishment of annual conference and Senior Faculty examination (l98l) as evidence of continued…

  10. An Interview with Dr. Maurizio Andolfi.

    Science.gov (United States)

    Cron, Elyce A.

    2000-01-01

    Presents an interview with Maurizio Andolfi, M.D., professor in the Department of Psychology at the University of Rome. He currently heads one of the most prestigious centers for training family therapists in Europe. The interview focuses on Andolfi's continuing professional and personal journeys. (GCP)

  11. CRISPR/Cas9系统的脱靶效应%Off-target of CRISPR/Cas9 System

    Institute of Scientific and Technical Information of China (English)

    尹珅; 贺桂芳; 赖方秾; 谢凤云; 马俊宇

    2016-01-01

    在细菌中发现的免疫系统CRISPR/Cas9,已经成为最有效的基因工程编辑工具,甚至大有希望可以治疗人类遗传性疾病。但是CRISPR/Cas9系统在使用时会产生严重的脱靶问题,导致假表型和错误的解释。提高与靶点结合的高效率,同时减少脱靶效应,将是今后CRISPR/Cas9技术的挑战。综述关注与CRISPR/Cas9脱靶效应相关的内容,总结了影响其靶点专一性的因素,减少CRISPR/Cas9脱靶效应的可行性方法和设计工具等,供大家学习讨论。%As an adaptive immune system discovered in bacteria,clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)technology has been used as the most effective genome-editing tool. By now,CRISPR/Cas9 has shown a great promise in healing human genetic diseases,however,off-target is a critical issue while applying it. The off-target of CRISPR/Cas9 leads to the false phenotype and wrong interpretation. Optimization of on-target activity and minimizing off-target activity will be challenges while CRISPR/Cas9 is applied in the future. In this review,we focused on the off-target related issues,including factors involved in the target specificity,strategies and tools of minimizing the off-target of CRISPR/Cas9.

  12. Pilomatricome: étude de 22 cas

    Science.gov (United States)

    Nasreddine, Fatima Zahra; Hali, Fouzia; Chiheb, Soumiya

    2016-01-01

    Le pilomatricome est une tumeur cutanée fréquente et bénigne du follicule pileux chez l'enfant. C'est une tumeur annexielle souvent méconnue et confondue avec d'autres lésions cutanées. Les localisations habituelles sont la tête et le cou. Le but de ce travail est de rapporter une série de 22 cas comportant des formes inhabituelles colligées au service de dermatologie sur une période allant de Janvier 2006 jusqu'au Mai 2015. L’étude a concerné 16 femmes et 6 hommes. La moyenne d’âge était de 23,3 ans (4-80 ans). La localisation cervico faciale a été observée dans 12 cas, 2 patients avaient des localisations multiples, un garçon de 4ans avait une localisation au niveau fronto-temporal et une fillette de 14 ans avait une localisation au niveau du visage et de l'avant-bras, et un patient de 48 ans avait une localisation sous unguéale. L'aspect clinique était typique dans tous les cas avec des nodules sous cutanés de consistance pierreuse. Tous les patients ont bénéficié d'une exérèse des nodules sous anesthésie locale. L’étude histologique était en faveur d'un épithélioma momifié de Malherbe d'exérèse complète sans signes de malignité. Aucun patient n'a présenté de rechute. L'originalité de notre étude réside dans la présence de localisations exceptionnelles au niveau latéro-vertébral, des membres et sous-unguéale, l’âge de survenue inhabituel à 80 ans et la présence de localisations multiples signalées chez 2 enfants. PMID:27516819

  13. Crystal Structure of Streptococcus pyogenes Cas1 and Its Interaction with Csn2 in the Type II CRISPR-Cas System.

    Science.gov (United States)

    Ka, Donghyun; Lee, Hasup; Jung, Yi-Deun; Kim, Kyunggon; Seok, Chaok; Suh, Nayoung; Bae, Euiyoung

    2016-01-01

    CRISPRs and Cas proteins constitute an RNA-guided microbial immune system against invading nucleic acids. Cas1 is a universal Cas protein found in all three types of CRISPR-Cas systems, and its role is implicated in new spacer acquisition during CRISPR-mediated adaptive immunity. Here, we report the crystal structure of Streptococcus pyogenes Cas1 (SpCas1) in a type II CRISPR-Cas system and characterize its interaction with S. pyogenes Csn2 (SpCsn2). The SpCas1 structure reveals a unique conformational state distinct from type I Cas1 structures, resulting in a more extensive dimerization interface, a more globular overall structure, and a disruption of potential metal-binding sites for catalysis. We demonstrate that SpCas1 directly interacts with SpCsn2, and identify the binding interface and key residues for Cas complex formation. These results provide structural information for a type II Cas1 protein, and lay a foundation for studying multiprotein Cas complexes functioning in type II CRISPR-Cas systems.

  14. Anchors for the Cosmic Distance Scale: the Cepheids U Sgr, CF Cas and CEab Cas

    CERN Document Server

    Majaess, D; Bidin, C Moni; Bonatto, C; Berdnikov, L; Balam, D; Moyano, M; Gallo, L; Turner, D; Lane, D; Gieren, W; Borissova, J; Kovtyukh, V; Beletsky, Y

    2013-01-01

    New and existing X-ray, UBVJHKsW(1-4), and spectroscopic observations were analyzed to constrain fundamental parameters for M25, NGC 7790, and dust along their sight-lines. The star clusters are of particular importance given they host the classical Cepheids U Sgr, CF Cas, and the visual binary Cepheids CEa and CEb Cas. Precise results from the multiband analysis, in tandem with a comprehensive determination of the Cepheids' period evolution (dP/dt) from ~140 years of observations, helped resolve concerns raised regarding the clusters and their key Cepheid constituents. Specifically, distances derived for members of M25 and NGC 7790 are 630+-25 pc and 3.40+-0.15 kpc, respectively.

  15. Space psychology

    Science.gov (United States)

    Parin, V. V.; Gorbov, F. D.; Kosmolinskiy, F. P.

    1974-01-01

    Psychological selection of astronauts considers mental responses and adaptation to the following space flight stress factors: (1) confinement in a small space; (2) changes in three dimensional orientation; (3) effects of altered gravity and weightlessness; (4) decrease in afferent nerve pulses; (5) a sensation of novelty and danger; and (6) a sense of separation from earth.

  16. Psychology Experiments.

    Science.gov (United States)

    McGraw, Ken; Tew, Mark D.; Williams, John E.

    2001-01-01

    A goal of the PsychExperiments project was to reduce the financial burden on psychology departments for hardware/software used in their laboratories. In its third year, the PsychExperiments site now hosts 39 experiments. Over 200 classrooms worldwide have signed up as official site users and there have been nearly 10,000 data sessions conducted.…

  17. Psychology Today.

    Science.gov (United States)

    Anderssen, Arthur

    2003-01-01

    Describes the design of the University of Texas Austin's Seay Psychology and Child Development & Family Relationships building. With modern technique and materials, the Seay building adds to the established architectural language of the campus, offering a richly tactile structure and adjoining outdoor space embracing the tenets of successful…

  18. Psychology Experiments.

    Science.gov (United States)

    McGraw, Ken; Tew, Mark D.; Williams, John E.

    2001-01-01

    A goal of the PsychExperiments project was to reduce the financial burden on psychology departments for hardware/software used in their laboratories. In its third year, the PsychExperiments site now hosts 39 experiments. Over 200 classrooms worldwide have signed up as official site users and there have been nearly 10,000 data sessions conducted.…

  19. Dr. Hans Chang, Director, Physics Research Committee, Stichting voor Fundamenteel Onderzoek der Materie (FOM), Dr. Joris Van Enst, Head of Science Policy Division, Ministry of Education, Culture and S cience, Dr. Jan Bezemer, NL Delegate CERN, Netherlands

    CERN Multimedia

    Patrice Loiez

    1999-01-01

    Dr. Hans Chang, Director, Physics Research Committee, Stichting voor Fundamenteel Onderzoek der Materie (FOM), Dr. Joris Van Enst, Head of Science Policy Division, Ministry of Education, Culture and S cience, Dr. Jan Bezemer, NL Delegate CERN, Netherlands

  20. CRISPR/Cas9-mediated viral interference in plants

    KAUST Repository

    Ali, Zahir

    2015-11-11

    Background The CRISPR/Cas9 system provides bacteria and archaea with molecular immunity against invading phages and conjugative plasmids. Recently, CRISPR/Cas9 has been used for targeted genome editing in diverse eukaryotic species. Results In this study, we investigate whether the CRISPR/Cas9 system could be used in plants to confer molecular immunity against DNA viruses. We deliver sgRNAs specific for coding and non-coding sequences of tomato yellow leaf curl virus (TYLCV) into Nicotiana benthamiana plants stably overexpressing the Cas9 endonuclease, and subsequently challenge these plants with TYLCV. Our data demonstrate that the CRISPR/Cas9 system targeted TYLCV for degradation and introduced mutations at the target sequences. All tested sgRNAs exhibit interference activity, but those targeting the stem-loop sequence within the TYLCV origin of replication in the intergenic region (IR) are the most effective. N. benthamiana plants expressing CRISPR/Cas9 exhibit delayed or reduced accumulation of viral DNA, abolishing or significantly attenuating symptoms of infection. Moreover, this system could simultaneously target multiple DNA viruses. Conclusions These data establish the efficacy of the CRISPR/Cas9 system for viral interference in plants, thereby extending the utility of this technology and opening the possibility of producing plants resistant to multiple viral infections.

  1. CRISPR/Cas9 in Genome Editing and Beyond.

    Science.gov (United States)

    Wang, Haifeng; La Russa, Marie; Qi, Lei S

    2016-06-01

    The Cas9 protein (CRISPR-associated protein 9), derived from type II CRISPR (clustered regularly interspaced short palindromic repeats) bacterial immune systems, is emerging as a powerful tool for engineering the genome in diverse organisms. As an RNA-guided DNA endonuclease, Cas9 can be easily programmed to target new sites by altering its guide RNA sequence, and its development as a tool has made sequence-specific gene editing several magnitudes easier. The nuclease-deactivated form of Cas9 further provides a versatile RNA-guided DNA-targeting platform for regulating and imaging the genome, as well as for rewriting the epigenetic status, all in a sequence-specific manner. With all of these advances, we have just begun to explore the possible applications of Cas9 in biomedical research and therapeutics. In this review, we describe the current models of Cas9 function and the structural and biochemical studies that support it. We focus on the applications of Cas9 for genome editing, regulation, and imaging, discuss other possible applications and some technical considerations, and highlight the many advantages that CRISPR/Cas9 technology offers.

  2. Chronic arsenic intoxication diagnostic score (CAsIDS).

    Science.gov (United States)

    Dani, Sergio Ulhoa; Walter, Gerhard Franz

    2017-08-31

    Arsenic and its compounds are well-established, potent, environmentally widespread and persistent toxicants with metabolic, genotoxic, mutagenic, teratogenic, epigenetic and carcinogenic effects. Arsenic occurs naturally in the Earth's crust, but anthropogenic arsenic emissions have surmounted the emissions from important natural sources such as volcanism. Inorganic arsenicals exhibit acute and chronic toxicities in virtually all cell types and tissues, and hence arsenic intoxication affects multiple systems. Whereas acute arsenic intoxication is rare and relatively easy to diagnose, chronic arsenic intoxication (CAsI) is common but goes often misdiagnosed. Based on a review of the literature as well as our own clinical experience, we propose a chronic arsenic intoxication diagnostic score (CAsIDS). A distinctive feature of CAsIDS is the use of bone arsenic load as an essential criterion for the individual risk assessment of chronic arsenic intoxication, combined with a systemic clinical assessment. We present clinical examples where CAsIDS is applied for the diagnosis of CAsI, review the main topics of the toxicity of arsenic in different cell and organ systems and discuss the therapy and prevention of disease caused or aggravated by chronic arsenic intoxication. CAsIDS can help physicians establish the diagnosis of CAsI and associated conditions. Copyright © 2017 John Wiley & Sons, Ltd.

  3. CRISPR/Cas9 Based Genome Editing of Penicillium chrysogenum.

    Science.gov (United States)

    Pohl, C; Kiel, J A K W; Driessen, A J M; Bovenberg, R A L; Nygård, Y

    2016-07-15

    CRISPR/Cas9 based systems have emerged as versatile platforms for precision genome editing in a wide range of organisms. Here we have developed powerful CRISPR/Cas9 tools for marker-based and marker-free genome modifications in Penicillium chrysogenum, a model filamentous fungus and industrially relevant cell factory. The developed CRISPR/Cas9 toolbox is highly flexible and allows editing of new targets with minimal cloning efforts. The Cas9 protein and the sgRNA can be either delivered during transformation, as preassembled CRISPR-Cas9 ribonucleoproteins (RNPs) or expressed from an AMA1 based plasmid within the cell. The direct delivery of the Cas9 protein with in vitro synthesized sgRNA to the cells allows for a transient method for genome engineering that may rapidly be applicable for other filamentous fungi. The expression of Cas9 from an AMA1 based vector was shown to be highly efficient for marker-free gene deletions.

  4. CAS Introduction to Accelerator Physics in Spain

    CERN Multimedia

    CERN Bulletin

    2012-01-01

    The CERN Accelerator School (CAS) and the University of Granada jointly organised a course called "Introduction to Accelerator Physics" in Granada, Spain, from 28 October to 9 November, 2012.   The course attracted over 200 applicants, of whom 139 were selected to attend. The students were of 25 different nationalities, coming from countries as far away as Australia, China, Guatemala and India. The intensive programme comprised 38 lectures, 3 seminars, 4 tutorials where the students were split into three groups, a poster session and 7 hours of guided and private study. Feedback from the students was very positive, praising the expertise of the lecturers, as well as the high standard and quality of their lectures. CERN's Director-General, Rolf Heuer, gave a public lecture at the Parque de las Ciencias entitled "The Large Hadron Collider: Unveiling the Universe". In addition to the academic programme, the students had the opportunity to visit the well...

  5. CAS course on Plasma Wake Acceleration

    CERN Multimedia

    CERN Accelerator School

    2015-01-01

    The CERN Accelerator School (CAS) recently organised a specialised course on Plasma Wake Acceleration, held at CERN, Geneva, Switzerland, from 23 to 29 November 2014.    Following a number of introductory lectures on laser and plasma physics, as well as an overview of conventional accelerators and their limitations, the course covered a large number of aspects of plasma wake acceleration schemes: the creation of plasma by high power lasers or particle beams, a description of the plasma creation process through simulations and the characteristics of the accelerated particle beams, including results of the latest achievements. Lectures on beam diagnostics, the applications of plasma accelerated beams, and topical seminars completed the programme.  The course was very successful, with 109 students of 26 nationalities attending; most participants coming from European counties, but also from the US, Israel, India, South Korea, Russia and Ukraine. Feedback from the participants was...

  6. CAS Accelerator Physics (Ion Sources) in Slovakia

    CERN Multimedia

    CAS School

    2012-01-01

    The CERN Accelerator School (CAS) and the Slovak University of Technology jointly organised a specialised course on ion sources, held at the Hotel Senec, Senec, Slovakia, from 29 May to 8 June, 2012.   Following some background lectures on accelerator physics and the fundamental processes of atomic and plasma physics, the course covered a wide range of topics related to ion sources and highlighted the latest developments in the field. Realistic case studies and topical seminars completed the programme. The school was very successful, with 69 participants representing 25 nationalities. Feedback from the participants was extremely positive, reflecting the high standard of the lectures. The case studies were performed with great enthusiasm and produced some excellent results. In addition to the academic programme, the participants were able to take part in a one-day excursion consisting of a guided tour of Bratislava and free time. A welcome event was held at the Hotel Senec, with s...

  7. CAS Accelerator Physics held in Erice, Italy

    CERN Multimedia

    CERN Accelerator School

    2013-01-01

    The CERN Accelerator School (CAS) recently organised a specialised course on Superconductivity for Accelerators, held at the Ettore Majorana Foundation and Centre for Scientific Culture in Erice, Italy from 24 April-4 May, 2013.   Photo courtesy of Alessandro Noto, Ettore Majorana Foundation and Centre for Scientific Culture. Following a handful of summary lectures on accelerator physics and the fundamental processes of superconductivity, the course covered a wide range of topics related to superconductivity and highlighted the latest developments in the field. Realistic case studies and topical seminars completed the programme. The school was very successful with 94 participants representing 23 nationalities, coming from countries as far away as Belorussia, Canada, China, India, Japan and the United States (for the first time a young Ethiopian lady, studying in Germany, attended this course). The programme comprised 35 lectures, 3 seminars and 7 hours of case study. The case studies were p...

  8. Adaptation in CRISPR-Cas Systems.

    Science.gov (United States)

    Sternberg, Samuel H; Richter, Hagen; Charpentier, Emmanuelle; Qimron, Udi

    2016-03-17

    Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) proteins constitute an adaptive immune system in prokaryotes. The system preserves memories of prior infections by integrating short segments of foreign DNA, termed spacers, into the CRISPR array in a process termed adaptation. During the past 3 years, significant progress has been made on the genetic requirements and molecular mechanisms of adaptation. Here we review these recent advances, with a focus on the experimental approaches that have been developed, the insights they generated, and a proposed mechanism for self- versus non-self-discrimination during the process of spacer selection. We further describe the regulation of adaptation and the protein players involved in this fascinating process that allows bacteria and archaea to harbor adaptive immunity. Copyright © 2016 Elsevier Inc. All rights reserved.

  9. Decommissioning of DR 2. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Strufe, N.

    2009-02-15

    This report describes the work of dismantling and demolishing reactor DR 2, the waste volumes generated, the health physical conditions and the clearance procedures used for removed elements and waste. Since the ultimate goal for the decommissioning project was not clearance of the building, but downgrading the radiological classification of the building with a view to converting it to further nuclear use, this report documents how the lower classification was achieved and the known occurrence of remaining activity. The report emphasises some of the deliberations made and describes the lessons learned through this decommissioning project. The report also intends to contribute towards the technical basis and experience basis for further decommissioning of the nuclear facilities in Denmark. (au)

  10. Generic Data Pipelining Using ORAC-DR

    Science.gov (United States)

    Allan, Alasdair; Jenness, Tim; Economou, Frossie; Currie, Malcolm J.; Bly, Martin J.

    A generic data reduction pipeline is, perhaps, the holy grail for data reduction software. We present work which sets us firmly on the path towards this goal. ORAC-DR is an online data reduction pipeline written by the Joint Astronomy Center (JAC) and the UK Astronomy Technology Center (ATC) and distributed as part of the Starlink Software collection (SSC). It is intended to run with a minimum of observer interaction, and is able to handle data from many different instruments, including SCUBA, CGS4, UFTI, IRCAM and Michelle, with support for IRIS2 and UIST under development. Recent work by Starlink in collaboration with the JAC has resulted in an increase in the pipeline's flexibility, opening up the possibility that it could be used for truly generic data reduction for data from any imaging, and eventually spectroscopic, detector.

  11. VIP Visit Her Excellency Dr Dalia Grybauskaite

    CERN Multimedia

    2016-01-01

    Her Excellency Dr Dalia Grybauskaite President Republic of Lithuania. Wednesday 20 January 2016. General introduction to CERN’s activities by CERN Director-General F. Gianotti. CERN Director-General introduces CMS Collaboration Deputy Spokesperson K. Borras and CMS Lithuanians A. Rinkevicius with V. Rapsevicius. Met by J. Shiers, IT Department Data Preservation Project Leader, and walk to 1st floor Data Centre Visit Point. CERN Data Centre Visit Point, 1st floor, building 513. View the robotic arms of the CERN IT data centre automated libraries (J. Shiers) CERN Computer Centre, building 513, level -1. Physics hands-on and virtual visit with a High School class in Lithuania (S. Schmeling) CERN S’cool Lab, building 143. Meeting with the Lithuanian community at CERN. Signature of the Guest Book with CERN Director-General witnessed by the Lithuanian community at CERN. Family photograph with the Lithuanian community at CERN.

  12. Dr Harold Frederick Shipman: an enigma.

    Science.gov (United States)

    Gunn, John

    2010-07-01

    Dr. Shipman was the worst known serial killer in British history, at least in terms of numbers of victims, and possibly the worst in world history, if politicians are excluded. He killed at least 215 patients and may have begun his murderous career at the age of 25, within a year of finishing his medical training. His case has had a profound impact on the practice of medicine in the United Kingdom. Was he a special case? What were the origins of this behaviour? Could the behaviour have been prevented? It is necessary to learn what we can from a few personal facts and largely circumstantial evidence. He withheld himself from any useful clinical investigation or treatment once he had been taken into custody. Could he have been treated at any stage?

  13. [Dr. John Baptiste Edouard Gélineau].

    Science.gov (United States)

    Janković, S; Susić, V; Sokić, D; Lević, Z

    1996-01-01

    With this brief review we honor the memory of the great French doctor Jean Baptiste Edouard Gélineau. Dr. Gélineau was born on December 23, 1828 at Blaye, Gironde, close to the Bordeaux region. His name is connected with the first clinical description of the disease for which he, both by the right of the primacy as well as ad valorem of his first two names, coined the name "narcolepsy". He was the first to notice the intrinsically evanescent symptoms of narcolepsy, such as excessive daytime somnolence, imperative sleep habits and cataplexy or "astasia" as he called it, and incorporate them into a single clinical syndrome. In 1881 Gélineau discussed Kaffe's case of "maladie du sommeil" as a proof of the existence of the new disease described a year before. As a good clinical observer Gélineau noticed the close relation of emotional engagement and astasia. His attitude was that narcolepsy was a nosologic entity, a disease sui generis, but admitted that it could appear purely as a symptom only. This was in discordance with the views in England where (in 1928) Dr. Samuel Alexander Kinnier Wilson repudiated such convictions; in 1930 Lhermitte still shared the same opinion. Gélineau differentiated narcolepsy from epilepsy with the elegance of clinical reasoning. Overall, Gélineau described three elements of the narcoleptic pentade. Sleep paralyses were first described by Mitchell in 1876, and were first attributed to narcolepsy by Wilson in 1928; in 1930 Lhermitte first described hypnapompic, and Daniels, in 1934, hypnagogic sleep paralysis. Hypnagogic hallucinations were described by Maury in 1848 and subsequently by de Saint Denis in 1867. In twenties they were thoroughly studiesed during the epidemic encephalitis and after the Big War in 1922 by Levy. The life story of Dr. Gélineau covers multivarious activities. As a young student of the Rochefort Navy Medical School he took part in the fight against colera which deluged the city of La Rochelle. In 1849 he

  14. Dr. Solco Tromp and the Tromp Award

    Science.gov (United States)

    Greene, J. Scott; Rietveld, Wop J.

    2017-09-01

    The Tromp Award is the highest honor awarded by the International Society of Biometeorology (ISB). The award acknowledges outstanding research in biometeorology by a scientist under the age of 35 and was established in conjunction with the Tromp Foundation and the ISB. In honor of the 60th anniversary of the ISB, this article will provide a brief summary of the life of Dr. Solco Tromp and of the six awardees of the Tromp Award since the inaugural issuance of the award in 1999. The Tromp Award was established in part to recognize and support the efforts of young biometeorological professionals. As the brief summary of the awardees and a few of their selected subsequent publications have shown, the ISB and the Tromp Award has proven effective at identifying and supporting promising young scientists.

  15. CRISPR/Cas9 advances engineering of microbial cell factories

    DEFF Research Database (Denmark)

    Jakociunas, Tadas; Jensen, Michael Krogh; Keasling, Jay D.

    2016-01-01

    interspaced palindromic repeats (CRISPR) and its associated proteins (Cas) have become the method of choice for precision genome engineering in many organisms due to their orthogonality, versatility and efficacy. Here we review the strategies adopted for implementation of RNA-guided CRISPR/Cas9 genome editing......-RNAs will be highlighted. Finally, this review will provide a perspective on the immediate challenges and opportunities foreseen by the use of CRISPR/Cas9 genome engineering and regulation in the context of metabolic engineering....

  16. Grossesse intra murale à propos d'un cas

    OpenAIRE

    de Tové, Kofi-Mensa Savi; Salifou, Kabibou; Imorou, Rachidi Sidi; Biaou, Olivier; Boco, Vicentia

    2015-01-01

    La grossesse intra-murale est la variété la plus rare de grossesse extra-utérine. Il s'agit de la localisation de l’œuf dans l’épaisseur du myomètre. En cas de retard diagnostic, l’évolution peut être catastrophique avec rupture utérine et hémorragie cataclysmique. L’échographie permet dans certains cas un diagnostic pré opératoire. Les auteurs rapportent un cas survenu chez une patiente aux antécédents de curetage.

  17. CAS Leaders Convene to Review Previous Work and Plan Future

    Institute of Scientific and Technical Information of China (English)

    2004-01-01

    @@ The 2004 enlarged summer session of the Leading Group of the Communist Party of China (CPC) at CAS was held July 26-31. It came amid a nationwide campaign to study the important thought of the Three Represents and the scientific approach to development advanced by the CPC Central Committee. The meeting coincided with a crucial moment for the development of CAS, as the Academy is about to kick off the third phase of work for the trial implementation of its Knowledge Innovation Program (KIP). A wide range of issues were discussed at this highly efficient and pragmatic meeting, which is expected to significantly influence the development of CAS.

  18. The 13th CAS General Assembly Convenes in Beijing

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    @@ The 13th General Assembly of CAS was held from June 5 to 8 in Beijing. Top State leaders were present at the joint opening session of the 13th CAS General Assembly and the Eighth General Assembly of the Chinese Academy of Engineering (CAE)held on the morning of June 5 at the Great Hall of the People. Chinese President HU Jintao delivered an important speech at the ceremony. State Councilor CHEN Zhili made a special report about the Framework for the National Long- and Mid-term Plan for S&T Development in China at the joint plenary session of CAS and CAE assemblies on June 6.

  19. Orbital period determination in an eclipsing dwarf nova HT Cas

    Science.gov (United States)

    Bąkowska, Karolina; Olech, Arkadiusz

    2014-09-01

    HT Cassiopeiae was discovered over seventy years ago (Hoffmeister 1943). Unfortunately, for 35 years this object did not receive any attention, until the eclipses of HT Cas were observed by Bond. After a first analysis, Patterson (1981) called HT Cas "a Rosetta stone among dwarf novae". Since then, the literature on this star is still growing, reaching several dozens of publications. We present an orbital period determination of HT Cas during the November 2010 super-outburst, but also during a longer time span, to check its stability.

  20. DR-model-based estimation algorithm for NCS

    Institute of Scientific and Technical Information of China (English)

    HUANG Si-niu; CHEN Zong-ji; WEI Chen

    2006-01-01

    A novel estimation scheme based on dead reckoning (DR) model for networked control system (NCS)is proposed in this paper.Both the detailed DR estimation algorithm and the stability analysis of the system are given.By using DR estimation of the state,the effect of communication delays is overcome.This makes a controller designed without considering delays still applicable in NCS Moreover,the scheme can effectively solve the problem of data packet loss or timeout.

  1. Goldie Brangman Remembers the Operation to Save Dr King.

    Science.gov (United States)

    Koch, Evan; Brangman, Goldie

    2015-12-01

    In September 1958 the Rev Dr Martin Luther King Jr was stabbed and nearly assassinated. Surgeons at Harlem Hospital in New York City removed a 17.8-cm (7-in)-long letter opener from Dr King's chest. Certified Registered Nurse Anesthetist Goldie Brangman remembers this event because she participated in Dr King's anesthetic. This article correlates Brangman's memories with published accounts of the event. It also places the event within the context of the modern civil rights movement that Dr King led.

  2. A guild of 45 CRISPR-associated (Cas protein families and multiple CRISPR/Cas subtypes exist in prokaryotic genomes.

    Directory of Open Access Journals (Sweden)

    Daniel H Haft

    2005-11-01

    Full Text Available Clustered regularly interspaced short palindromic repeats (CRISPRs are a family of DNA direct repeats found in many prokaryotic genomes. Repeats of 21-37 bp typically show weak dyad symmetry and are separated by regularly sized, nonrepetitive spacer sequences. Four CRISPR-associated (Cas protein families, designated Cas1 to Cas4, are strictly associated with CRISPR elements and always occur near a repeat cluster. Some spacers originate from mobile genetic elements and are thought to confer "immunity" against the elements that harbor these sequences. In the present study, we have systematically investigated uncharacterized proteins encoded in the vicinity of these CRISPRs and found many additional protein families that are strictly associated with CRISPR loci across multiple prokaryotic species. Multiple sequence alignments and hidden Markov models have been built for 45 Cas protein families. These models identify family members with high sensitivity and selectivity and classify key regulators of development, DevR and DevS, in Myxococcus xanthus as Cas proteins. These identifications show that CRISPR/cas gene regions can be quite large, with up to 20 different, tandem-arranged cas genes next to a repeat cluster or filling the region between two repeat clusters. Distinctive subsets of the collection of Cas proteins recur in phylogenetically distant species and correlate with characteristic repeat periodicity. The analyses presented here support initial proposals of mobility of these units, along with the likelihood that loci of different subtypes interact with one another as well as with host cell defensive, replicative, and regulatory systems. It is evident from this analysis that CRISPR/cas loci are larger, more complex, and more heterogeneous than previously appreciated.

  3. A Guild of 45 CRISPR-Associated (Cas Protein Families and Multiple CRISPR/Cas Subtypes Exist in Prokaryotic Genomes.

    Directory of Open Access Journals (Sweden)

    2005-11-01

    Full Text Available Clustered regularly interspaced short palindromic repeats (CRISPRs are a family of DNA direct repeats found in many prokaryotic genomes. Repeats of 21-37 bp typically show weak dyad symmetry and are separated by regularly sized, nonrepetitive spacer sequences. Four CRISPR-associated (Cas protein families, designated Cas1 to Cas4, are strictly associated with CRISPR elements and always occur near a repeat cluster. Some spacers originate from mobile genetic elements and are thought to confer "immunity" against the elements that harbor these sequences. In the present study, we have systematically investigated uncharacterized proteins encoded in the vicinity of these CRISPRs and found many additional protein families that are strictly associated with CRISPR loci across multiple prokaryotic species. Multiple sequence alignments and hidden Markov models have been built for 45 Cas protein families. These models identify family members with high sensitivity and selectivity and classify key regulators of development, DevR and DevS, in Myxococcus xanthus as Cas proteins. These identifications show that CRISPR/cas gene regions can be quite large, with up to 20 different, tandem-arranged cas genes next to a repeat cluster or filling the region between two repeat clusters. Distinctive subsets of the collection of Cas proteins recur in phylogenetically distant species and correlate with characteristic repeat periodicity. The analyses presented here support initial proposals of mobility of these units, along with the likelihood that loci of different subtypes interact with one another as well as with host cell defensive, replicative, and regulatory systems. It is evident from this analysis that CRISPR/cas loci are larger, more complex, and more heterogeneous than previously appreciated.

  4. Cultural psychology.

    Science.gov (United States)

    Heine, Steven J; Ruby, Matthew B

    2010-03-01

    Humans are a cultural species, constantly navigating a complex web of culturally bound practices, norms, and worldviews. This article provides a brief overview of the relatively young field of cultural psychology, which investigates the many ways psychology and culture interweave with one another. Highlighting the cultural nature of the human species, it draws upon research on cultural evolution, enculturation, and developmental processes. This review further summarizes a number of cultural differences in how people perceive the self, and the behavioral consequences that follow from these differences, in the domains of internal and external attribution styles, motivations for self-enhancement, approach/avoidance, primary and secondary control, as well as motivations for distinctiveness and conformity. Additionally, the review discusses research on the intersection of culture and emotion, as well as cultural differences in cognition, perception, and reasoning. Copyright © 2010 John Wiley & Sons, Ltd. For further resources related to this article, please visit the WIREs website. Copyright © 2010 John Wiley & Sons, Ltd.

  5. Revisiting the Hubble sequence in the SDSS DR7 spectroscopic sample: a publicly available bayesian automated classification

    CERN Document Server

    Huertas-Company, Marc; Bernardi, M; Mei, S; Almeida, J Sánchez

    2010-01-01

    We present an automated morphological classification in 4 types (E,S0,Sab,Scd) of ~700.000 galaxies from the SDSS DR7 spectroscopic sample based on support vector machines. The main new property of the classification is that we associate to each galaxy a probability of being in the four morphological classes instead of assigning a single class. The classification is therefore better adapted to nature where we expect a continuos transition between different morphological types. The algorithm is trained with a visual classification and then compared to several independent visual classifications including the Galaxy Zoo first release catalog. We find a very good correlation between the automated classification and classical visual ones. The compiled catalog is intended for use in different applications and can be downloaded at http://gepicom04.obspm.fr/sdss_morphology/Morphology_2010.html and soon from the CasJobs database.

  6. Analysis on the psychological characteristics of patients with acute iridocyclitis

    Institute of Scientific and Technical Information of China (English)

    Wei; Sun; Fan; Wu; Jun; Kong; Yukiko; Nakahara; Xiao-Yan; Li

    2015-01-01

    <正>Dear Sir,I am Dr.Wei Sun,from the Department of Ophthalmology,the Fourth Affiliated Hospital of China Medical University,Eye Hospital of China Medical University,shenyang,Liaoning Province,China.I write to present our study on the psychological characteristics of patients with acute iridocyclitis.Studies showed stimulation from patient’s external environment could influence their psychological state,persisting neural endocrine disorders that might lead to a series of physiological and psychological changes in the human body[1-6].It was confirmed many ocular diseases were

  7. CRISPR-Cas systems: Prokaryotes upgrade to adaptive immunity.

    Science.gov (United States)

    Barrangou, Rodolphe; Marraffini, Luciano A

    2014-04-24

    Clustered regularly interspaced short palindromic repeats (CRISPR), and associated proteins (Cas) comprise the CRISPR-Cas system, which confers adaptive immunity against exogenic elements in many bacteria and most archaea. CRISPR-mediated immunization occurs through the uptake of DNA from invasive genetic elements such as plasmids and viruses, followed by its integration into CRISPR loci. These loci are subsequently transcribed and processed into small interfering RNAs that guide nucleases for specific cleavage of complementary sequences. Conceptually, CRISPR-Cas shares functional features with the mammalian adaptive immune system, while also exhibiting characteristics of Lamarckian evolution. Because immune markers spliced from exogenous agents are integrated iteratively in CRISPR loci, they constitute a genetic record of vaccination events and reflect environmental conditions and changes over time. Cas endonucleases, which can be reprogrammed by small guide RNAs have shown unprecedented potential and flexibility for genome editing and can be repurposed for numerous DNA targeting applications including transcriptional control.

  8. Rewiring Cas9 to Target New PAM Sequences.

    Science.gov (United States)

    Siksnys, Virginijus; Gasiunas, Giedrius

    2016-03-17

    Papers by Anders et al. (2016) and Hirano et al. (2016b), published in this issue of Molecular Cell, show that SpCas9 uses an induced fit mechanism to recognize altered protospacer adjacent motif (PAM) sequences.

  9. CRISPR-Cas : revolutionising genome engineering : in practice - clinical update

    National Research Council Canada - National Science Library

    Pepper, M.S; Nicholson, S.A

    2016-01-01

    ... technologies, genome engineering looks set to become a clinical reality in the near future. This article discusses recent advancements in the technology called 'clustered regularly interspaced palindromic repeat (CRISPR)-associated genes' (CRISPR-Cas...

  10. Application of CRISPR/Cas9 for biomedical discoveries.

    Science.gov (United States)

    Riordan, Sean M; Heruth, Daniel P; Zhang, Li Q; Ye, Shui Qing

    2015-01-01

    The Clustered Regions of Interspersed Palindromic Repeats-Cas9 (CRISPR/Cas9), a viral defense system found in bacteria and archaea, has emerged as a tour de force genome editing tool. The CRISPR/Cas9 system is much easier to customize and optimize because the site selection for DNA cleavage is guided by a short sequence of RNA rather than an engineered protein as in the systems of zinc finger nucleases (ZFN), transcription activator-like effector nucleases (TALEN), and meganucleases. Although it still suffers from some off-target effects, the CRISPR/Cas9 system has been broadly and successfully applied for biomedical discoveries in a number of areas. In this review, we present a brief history and development of the CRISPR system and focus on the application of this genome editing technology for biomedical discoveries. We then present concise concluding remarks and future directions for this fast moving field.

  11. EU Commissioner Delivers a Speech at CAS Institute

    Institute of Scientific and Technical Information of China (English)

    2004-01-01

    @@ European Union (EU) Environment Commissioner Margot Wallstrom gave a talk on cooperation between the EU and China on environmental issues during her trip to the CAS Institute of Geographic Sciences and Natural Resource Research (IGSNRR) on Nov. 13.

  12. CRISPR-Cas9-Guided Genome Engineering in C. elegans.

    Science.gov (United States)

    Kim, Hyun-Min; Colaiácovo, Monica P

    2016-07-01

    The CRISPR (clustered regularly interspaced short palindromic repeats)-Cas (CRISPR-associated) system is successfully being used for efficient and targeted genome editing in various organisms, including the nematode C. elegans. Recent studies have developed various CRISPR-Cas9 approaches to enhance genome engineering via two major DNA double-strand break repair pathways: non-homologous end joining and homologous recombination. Here we describe a protocol for Cas9-mediated C. elegans genome editing together with single guide RNA (sgRNA) and repair template cloning, as well as injection methods required for delivering Cas9, sgRNAs, and repair template DNA into the C. elegans germline. © 2016 by John Wiley & Sons, Inc.

  13. CAS-EU researchers jointly explore integrated water resources management

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    @@ Under the organization of the CAS Institute of Tibetan Plateau Research (ITP),an exploration team composed of researchers from Germany, Austria, Czech, and Nepal carried out studies on the Yarlung Zangbo River Basin from Sept. 12 to 26.

  14. CRISPR-Cas systems: prokaryotes upgrade to adaptive immunity

    Science.gov (United States)

    Barrangou, Rodolphe; Marraffini, Luciano A.

    2014-01-01

    Summary Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR), and associated proteins (Cas) comprise the CRISPR-Cas system, which confers adaptive immunity against exogenic elements in many bacteria and most archaea. CRISPR-mediated immunization occurs through the uptake of DNA from invasive genetic elements such as plasmids and viruses, followed by its integration into CRISPR loci. These loci are subsequently transcribed and processed into small interfering RNAs that guide nucleases for specific cleavage of complementary sequences. Conceptually, CRISPR-Cas shares functional features with the mammalian adaptive immune system, while also exhibiting characteristics of Lamarckian evolution. Because immune markers spliced from exogenous agents are integrated iteratively in CRISPR loci, they constitute a genetic record of vaccination events and reflect environmental conditions and changes over time. Cas endonucleases, which can be reprogrammed by small guide RNAs have shown unprecedented potential and flexibility for genome editing, and can be repurposed for numerous DNA targeting applications including transcriptional control. PMID:24766887

  15. Methods for Optimizing CRISPR-Cas9 Genome Editing Specificity.

    Science.gov (United States)

    Tycko, Josh; Myer, Vic E; Hsu, Patrick D

    2016-08-04

    Advances in the development of delivery, repair, and specificity strategies for the CRISPR-Cas9 genome engineering toolbox are helping researchers understand gene function with unprecedented precision and sensitivity. CRISPR-Cas9 also holds enormous therapeutic potential for the treatment of genetic disorders by directly correcting disease-causing mutations. Although the Cas9 protein has been shown to bind and cleave DNA at off-target sites, the field of Cas9 specificity is rapidly progressing, with marked improvements in guide RNA selection, protein and guide engineering, novel enzymes, and off-target detection methods. We review important challenges and breakthroughs in the field as a comprehensive practical guide to interested users of genome editing technologies, highlighting key tools and strategies for optimizing specificity. The genome editing community should now strive to standardize such methods for measuring and reporting off-target activity, while keeping in mind that the goal for specificity should be continued improvement and vigilance.

  16. Engineering the Caenorhabditis elegans genome with CRISPR/Cas9.

    Science.gov (United States)

    Waaijers, Selma; Boxem, Mike

    2014-08-01

    The development in early 2013 of CRISPR/Cas9-based genome engineering promises to dramatically advance our ability to alter the genomes of model systems at will. A single, easily produced targeting RNA guides the Cas9 endonuclease to a specific DNA sequence where it creates a double strand break. Imprecise repair of the break can yield mutations, while homologous recombination with a repair template can be used to effect specific changes to the genome. The tremendous potential of this system led several groups to independently adapt it for use in Caenorhabditiselegans, where it was successfully used to generate mutations and to create tailored genome changes through homologous recombination. Here, we review the different approaches taken to adapt CRISPR/Cas9 for C. elegans, and provide practical guidelines for CRISPR/Cas9-based genome engineering.

  17. Editing plant genomes with CRISPR/Cas9.

    Science.gov (United States)

    Belhaj, Khaoula; Chaparro-Garcia, Angela; Kamoun, Sophien; Patron, Nicola J; Nekrasov, Vladimir

    2015-04-01

    CRISPR/Cas9 is a rapidly developing genome editing technology that has been successfully applied in many organisms, including model and crop plants. Cas9, an RNA-guided DNA endonuclease, can be targeted to specific genomic sequences by engineering a separately encoded guide RNA with which it forms a complex. As only a short RNA sequence must be synthesized to confer recognition of a new target, CRISPR/Cas9 is a relatively cheap and easy to implement technology that has proven to be extremely versatile. Remarkably, in some plant species, homozygous knockout mutants can be produced in a single generation. Together with other sequence-specific nucleases, CRISPR/Cas9 is a game-changing technology that is poised to revolutionise basic research and plant breeding.

  18. Signing Ceremony Between CAS and Schneider Electric Held in Beijing

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    @@ On April 9, 2008, a strategic cooperation signing ceremony between the China Association for Stan-dardization (CAS) and Schneider Electric took place at the Diao Yutai Guest Hotel in Beijing. Attending the signing ceremony were Mr Sun Xiaokang, Duty Director of SAC, Mr. Ma Lingcong, Secretary General of CAS, Mr. Noel Girard, Schneider Electric Marketing Director, Mr. Guy Dufrdi sse, Chairman of Schneider Electric (China) and more than twenty high-ranking managers from both companies.

  19. CAS sets up an award to promote international cooperation

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    @@ Established in 2007, the CAS Award for International Cooperation in Science and Technology is to honor those eminent international experts who have made outstanding contributions to China's global cooperation in science and technology, so as to encourage more efforts in this respect that will lead to the enhancement of CAS innovation capacity and the improvement of its research performance, education and training, management and reputations among the international community.

  20. CAS spearheads R&D program for research facilities

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    @@ China's capacity for indigenous S&T innovation is believed to have been hampered by its lack of home- grown research facilities. To address the problem, a pilot program for the research and development of major S&T facilities has been launched at CAS. The kick-off meeting was held on 28 March in the CAS Technical Institute of Physics and Chemistry in Beijing.

  1. CAS-The Prospect of Work in 2006

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    @@ Mission Statement China Association for Standardization (CAS) is to act as a bridge linking nationwide specialists in standardization with social communities. To meet the needs of government,society, market and enterprises, CAS endeavors to disseminate and popularize the standardization knowledge, organize academic forums and seminars, provide technical consultation on standardization, promote domestic and international cooperation and exchanges,and push forward the standardization process in China.

  2. Regulations for CAS Visiting Professorships for Senior International Scientists

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    @@ 1.General Provisions. Article 1 These regulations are made in accordance with the Chinese Academy of Sciences (CAS) "Package Program for Talent Training & Recruitment" and "Guidelines of the Chinese Academy of Sciences for the Implementation of the Program for Attracting Overseas Scientists and Experts and Cultivating Talent through International Exchange",to guide the implementation of the "CAS Visiting Professorships for Senior International Scientists" (hereinafter referred to as the "Visiting Professorships Program").

  3. CAS to deepen scientific cooperation with enterprises and localities

    Institute of Scientific and Technical Information of China (English)

    2012-01-01

    On April 10, 2012, an overall strategic partnership agreement between CAS and China Petrochemical Corporation (Sinopec Group) was signed by CAS President BAI Chunli and Chairman of Sinopec Group FU Chengyu, launching collaborations in six key areas including synchrotron radiation technologies, thorium-based molten salt reactor system, and the preparation of basic chemicals via coal-based syngas and high temperature electrolysis.

  4. Winners of 2008 CAS Award for international Scientific Cooperation

    Institute of Scientific and Technical Information of China (English)

    XIN Ling

    2009-01-01

    @@ On 14 January, 2009, CAS honored three foreign scientists for their lasting and fruitful contributions to the collaboration between CAS and the world: Akito Arima, president of the Japan Science Foundation, Yuen-Ron Shen, professor of physics with the University of California at Berkley, and Michel Che, catalyst expert and chair professor at Universite Pierre et Marie Curie. Their association with the Chinese scientific community can be dated back to some three decades ago.

  5. CAS projects winning National Natural Science Awards in 2008

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    @@ Editor's Note: At the 2008 National Conference for Science and Technology Awards held earlier this year in Beijing, CAS scientists were honored with 17 second prizes of the National Natural Science Awards, 3 prizes of the Awards for Technical Invention and 14 prizes for National Awards for S&T Progress, respectively. The following is a brief introduction to some CAS projects honored by the National Natural Science Awards.

  6. CRISPR-Cas9: A Revolutionary Tool for Cancer Modelling

    OpenAIRE

    Raul Torres-Ruiz; Sandra Rodriguez-Perales

    2015-01-01

    The cancer-modelling field is now experiencing a conversion with the recent emergence of the RNA-programmable CRISPR-Cas9 system, a flexible methodology to produce essentially any desired modification in the genome. Cancer is a multistep process that involves many genetic mutations and other genome rearrangements. Despite their importance, it is difficult to recapitulate the degree of genetic complexity found in patient tumors. The CRISPR-Cas9 system for genome editing has been proven as a ...

  7. Target specificity of the CRISPR-Cas9 system

    OpenAIRE

    Wu, Xuebing; Kriz, Andrea J.; Sharp, Phillip A.

    2014-01-01

    The CRISPR-Cas9 system, naturally a defense mechanism in prokaryotes, has been repurposed as an RNA-guided DNA targeting platform. It has been widely used for genome editing and transcriptome modulation, and has shown great promise in correcting mutations in human genetic diseases. Off-target effects are a critical issue for all of these applications. Here we review the current status on the target specificity of the CRISPR-Cas9 system.

  8. CAS Held the 2nd Yearly Official Business Conference

    Institute of Scientific and Technical Information of China (English)

    2004-01-01

    @@ On July 15th 2004, China Association For Standardization (CAS) held official business conference the second time of this year in Beijing, which is its 5th Council Meeting. The conference was moderated by Li Rui, President of CAS's Board of Directors. Vice-presidents Li- Zhonghai, JiaoYunqi and Jing Xiaodong, Secretary-General Ma Lincong and Zhang Wei, and other cadres totally more than 20delegates attended the conference.

  9. Psychology of religion: perspectives from cultural psychology

    NARCIS (Netherlands)

    Belzen, J.A.

    2010-01-01

    After a brief introduction, this paper tries to establish what type of psychology the psychology of religion is. Having introduced cultural psychology in general, some theories applicable in research on religion are presented, and some examples of cultural psychological research of religious

  10. Psychology Faculty Perceptions of Abnormal Psychology Textbooks

    Science.gov (United States)

    Rapport, Zachary

    2011-01-01

    The problem. The purpose of the current study was to investigate the perceptions and opinions of psychology professors regarding the accuracy and inclusiveness of abnormal psychology textbooks. It sought answers from psychology professors to the following questions: (1) What are the expectations of the psychology faculty at a private university of…

  11. Enrico Fermi Awards Ceremony for Dr. Allen J. Bard and Dr. Andrew Sessler, February 2014 (Presentations, including remarks by Energy Secretary, Dr. Ernest Moniz)

    Energy Technology Data Exchange (ETDEWEB)

    Moniz, Ernest [U.S. Energy Secretary

    2014-02-03

    The Fermi Award is a Presidential award and is one of the oldest and most prestigious science and technology honors bestowed by the U.S. Government. On February 3, 2014 it was conferred upon two exceptional scientists. The first to be recognized is Dr. Allen J. Bard, 'for international leadership in electrochemical science and technology, for advances in photoelectrochemistry and photocatalytic materials, processes, and devices, and for discovery and development of electrochemical methods including electrogenerated chemiluminescence and scanning electrochemical microscopy.' The other honoree is Dr. Andrew Sessler, 'for advancing accelerators as powerful tools of scientific discovery, for visionary direction of the research enterprise focused on challenges in energy and the environment, and for championing outreach and freedom of scientific inquiry worldwide.' Dr. Patricia Dehmer opened the ceremony, and Dr. Ernest Moniz presented the awards.

  12. Rational design of a split-Cas9 enzyme complex

    Science.gov (United States)

    Wright, Addison V.; Sternberg, Samuel H.; Taylor, David W.; Staahl, Brett T.; Bardales, Jorge A.; Kornfeld, Jack E.; Doudna, Jennifer A.

    2015-01-01

    Cas9, an RNA-guided DNA endonuclease found in clustered regularly interspaced short palindromic repeats (CRISPR) bacterial immune systems, is a versatile tool for genome editing, transcriptional regulation, and cellular imaging applications. Structures of Streptococcus pyogenes Cas9 alone or bound to single-guide RNA (sgRNA) and target DNA revealed a bilobed protein architecture that undergoes major conformational changes upon guide RNA and DNA binding. To investigate the molecular determinants and relevance of the interlobe rearrangement for target recognition and cleavage, we designed a split-Cas9 enzyme in which the nuclease lobe and α-helical lobe are expressed as separate polypeptides. Although the lobes do not interact on their own, the sgRNA recruits them into a ternary complex that recapitulates the activity of full-length Cas9 and catalyzes site-specific DNA cleavage. The use of a modified sgRNA abrogates split-Cas9 activity by preventing dimerization, allowing for the development of an inducible dimerization system. We propose that split-Cas9 can act as a highly regulatable platform for genome-engineering applications. PMID:25713377

  13. Editing plants for virus resistance using CRISPR-Cas.

    Science.gov (United States)

    Green, J C; Hu, J S

    This minireview summarizes recent advancements using the clustered regularly interspaced palindromic repeats-associated nuclease systems (CRISPR-Cas) derived from prokaryotes to breed plants resistant to DNA and RNA viruses. The CRISPR-Cas system represents a powerful tool able to edit and insert novel traits into plants precisely at chosen loci offering enormous advantages to classical breeding. Approaches to engineering plant virus resistance in both transgenic and non-transgenic plants are discussed. Iterations of the CRISPR-Cas system, FnCas9 and C2c2 capable of editing RNA in eukaryotic cells offer a particular advantage for providing resistance to RNA viruses which represent the great majority of known plant viruses. Scientists have obtained conflicting results using gene silencing technology to produce transgenic plants resistant to geminiviruses. CRISPR-Cas systems engineered in plants to target geminiviruses have consistently reduced virus accumulation providing increased resistance to virus infection. CRISPR-Cas may provide novel and reliable approaches to control geminiviruses and other ssDNA viruses such as Banana bunchy top virus (BBTV).

  14. CAS Presents '09 Outstanding S&T Achievement Awards

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    @@ CAS announced winners of its 2009 Science & Technology Achievement Awards on January27,2010 in Beijing. Five research groups were conferred on the awards,namely,the group for the heavy ion research facility in Lanzhou-cooler storage ring(HIRFL-CSR)with the CAS Institute of Modern Physics(IMP),the group for theoretical research into regional space development and practical research into major planning in China with the CAS Institute of Geographic Sciences and Natural Resources Research,the group for the world's first"synthesis glycol from coal"technology with the CAS Fujian Institute of Research on the Structure of Matter,the group for the concomitant satellite for"Shenzhou-Ⅶ"-China's third manned spacecraft-with the CAS Shanghai Engineering Center for Microsatellites,and the group for the visualized casting-forging technology with the CAS Institute of Metal Research.The biennial awards were founded in 2002 to honor individuals and research groups with significant research achievements in the latest five years.Less than 10 individuals or research groups are awarded each time.

  15. CRISPR-Cas9 Based Engineering of Actinomycetal Genomes.

    Science.gov (United States)

    Tong, Yaojun; Charusanti, Pep; Zhang, Lixin; Weber, Tilmann; Lee, Sang Yup

    2015-09-18

    Bacteria of the order Actinomycetales are one of the most important sources of pharmacologically active and industrially relevant secondary metabolites. Unfortunately, many of them are still recalcitrant to genetic manipulation, which is a bottleneck for systematic metabolic engineering. To facilitate the genetic manipulation of actinomycetes, we developed a highly efficient CRISPR-Cas9 system to delete gene(s) or gene cluster(s), implement precise gene replacements, and reversibly control gene expression in actinomycetes. We demonstrate our system by targeting two genes, actIORF1 (SCO5087) and actVB (SCO5092), from the actinorhodin biosynthetic gene cluster in Streptomyces coelicolor A3(2). Our CRISPR-Cas9 system successfully inactivated the targeted genes. When no templates for homology-directed repair (HDR) were present, the site-specific DNA double-strand breaks (DSBs) introduced by Cas9 were repaired through the error-prone nonhomologous end joining (NHEJ) pathway, resulting in a library of deletions with variable sizes around the targeted sequence. If templates for HDR were provided at the same time, precise deletions of the targeted gene were observed with near 100% frequency. Moreover, we developed a system to efficiently and reversibly control expression of target genes, deemed CRISPRi, based on a catalytically dead variant of Cas9 (dCas9). The CRISPR-Cas9 based system described here comprises a powerful and broadly applicable set of tools to manipulate actinomycetal genomes.

  16. Interview with Smithsonian NASM Spacesuit Curator Dr. Cathleen Lewis

    Science.gov (United States)

    Lewis, Cathleen; Wright, Rebecca

    2012-01-01

    Dr. Cathleen Lewis was interviewed by Rebecca Wright during the presentation of an "Interview with Smithsonian NASM Spacesuit Curator Dr. Cathleen Lewis" on May 14, 2012. Topics included the care, size, and history of the spacesuit collection at the Smithsonian and the recent move to the state-of-the-art permanent storage facility at the Udvar-Hazy facility in Virginia.

  17. Dr. Shawn Mehlenbacher 2012 Wilder Silver Medal Recipient

    Science.gov (United States)

    Dr. Shawn Mehlenbacher, Oregon State University, was awarded the 2012 Wilder Medal by the American Pomological Society for his contributions to hazelnut genetics and cultivar development. Dr. Mehlenbacher took over the leadership of the Oregon State University hazelnut breeding program in 1986 aft...

  18. Dr. E. Kits van Waveren (1906—1995)

    NARCIS (Netherlands)

    Bas, C.

    1996-01-01

    With the death on 3 September 1995 of Dr. E. Kits van Waveren, the Dutch mycologists lost one of their most prominent, internationally known amateurs, a specialist on the taxonomy of the genus Psathyrella and an ardent collector. Dr. Kits van Waveren, honorary staff member of the Rijksherbarium at L

  19. Dr. Praveen Chaudhari named director of Brookhaven National Laboratory

    CERN Document Server

    2003-01-01

    "Brookhaven Science Associates announced today the selection of Dr. Praveen Chaudhari as Director of the U.S. Department of Energy's Brookhaven National Laboratory. Dr. Chaudhari, who will begin his new duties on April 1, joins Brookhaven Lab after 36 years of distinguished service at IBM as a scientist and senior manager of research" (1 page).

  20. Celebrating the Life and Legacy of Dr. John Hope Franklin

    Science.gov (United States)

    Harris, Robert L., Jr.; Levering-Lewis, David; French, John D.; Wharton, Clifton R., Jr.

    2009-01-01

    Dr. John Hope Franklin chronicled the experiences of African-Americans like no one before him, forcing America to recognize Black history as American history. His contributions were innumerable and his impact was abiding. In celebration of his life and legacy, the authors profile the celebrated scholar and activist, Dr. John Hope Franklin.

  1. Efficacy of DR, CT and MRI in bone tumors

    Institute of Scientific and Technical Information of China (English)

    Yanjiao Zhang; Xuefeng Cui; Changcheng Li; Shenjiang Li

    2014-01-01

    Objective:The aim of the study was to investigate the ef icacy of digital radiography (DR), computed tomography (CT) and magnetic resonance imaging (MRI) in bone tumors. Methods:Fifty-five patients with histological y confirmed bone tumors underwent imaging examinations. Fifty-five patients were performed DR, 21 CT and 20 MRI. Results:DR of 51 pa-tients clearly revealed bone changes. DR of 40 patients wel showed marginal. Twenty tumors appeared periosteal reaction on DR and 19 calcification on DR. CT scan of 21 patients clearly depicted bone changes, marginal and calcification and CT scan of 8 revealed periosteal reaction. MRI scan of 20 patients al showed marginal and soft-tissue mass and bone marrow edema was prominent in 8 patients. Conclusion:DR is the first imaging approach for born tumors. CT wel reveal the extent and minute structure of the bone tumors, the extent, soft-tissue mass and bone marrow edema are more dramatical y demon-strated on MRI imaging. DR integrates with CT and MRI, is helpful in diagnosis of bone tumors.

  2. Dr. Lai Xinsheng's Experience in Acupuncture Treatment of Refractory Diseases

    Institute of Scientific and Technical Information of China (English)

    Lin Shaozhen; Lai Xinsheng; Duan Shumin

    2006-01-01

    @@ Having been engaged in teaching, clinical practice and research for many years, Dr. Lai Xinsheng is good at acupuncture and the combined acupuncture and medication therapy to treat refractory diseases with satisfactory therapeutic effect. Sample cases treated by Dr. Lai are selected as follows.

  3. Dr. Albert Carr--Science Educator 1930-2000

    Science.gov (United States)

    Lopez, Leslie

    2013-01-01

    The very first issue of "Educational Perspectives" was published in October of 1962. Dr. Albert Carr wrote one of the inaugural essays on the topic of current developments in science education, and he went on to write several other articles for the journal. This article shares why Dr. Albert Carr's colleagues remember him for his…

  4. Balance Between the Worlds: A Conversation with Dr. Richard Vedan

    Directory of Open Access Journals (Sweden)

    Carolyn B Kenny

    2015-11-01

    Full Text Available In this article, Dr. Richard Vedan, a Secwepmec, lodge keeper, and medical social worker converses with Dr. Carolyn Kenny about critical elements of medical ethnomusicology as seen and experienced through an Indigenous context. Dilemmas of individualism versus collectivism and isolation versus connectivity underlie the entire conversation. Relevant themes in the traditional use of music in Indigenous healing are discussed.

  5. SDSS DR7 White Dwarf Catalog

    CERN Document Server

    Kleinman, S J; Koester, D; Pelisoli, Ingrid; Peçanha, Viviane; Nitta, A; Costa, J E S; Krzesinski, J; Dufour, P; Lachapelle, F -R; Bergeron, P; Yip, Ching-Wa; Harris, Hugh C; Eisenstein, Daniel J; Althaus, L; Córsico, A

    2012-01-01

    We present a new catalog of spectroscopically-confirmed white dwarf stars from the Sloan Digital Sky Survey Data Release 7 spectroscopic catalog. We find 20,407 white dwarf spectra, representing 19,712 stars, and provide atmospheric model fits to 14,120 DA and 1011 DB white dwarf spectra from 12,843 and 923 stars, respectively. These numbers represent a more than factor of two increase in the total number of white dwarf stars from the previous SDSS white dwarf catalog based on DR4 data. Our distribution of subtypes varies from previous catalogs due to our more conservative, manual classifications of each star in our catalog, supplementing our automatic fits. In particular, we find a large number of magnetic white dwarf stars whose small Zeeman splittings mimic increased Stark broadening that would otherwise result in an overestimated log(g) if fit as a non-magnetic white dwarf. We calculate mean DA and DB masses for our clean, non-magnetic sample and find the DB mean mass is statistically larger than that for...

  6. SDSS DR7 WHITE DWARF CATALOG

    Energy Technology Data Exchange (ETDEWEB)

    Kleinman, S. J.; Nitta, A. [Gemini Observatory, 670 North A' ohoku Place, Hilo, HI 96720 (United States); Kepler, S. O.; Pelisoli, Ingrid; Pecanha, Viviane; Costa, J. E. S. [Instituto de Fisica, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS (Brazil); Koester, D. [Institut fuer Theoretische Physik und Astrophysik, Universitaet Kiel, D-24098 Kiel (Germany); Krzesinski, J. [Mt. Suhora Observatory, Pedagogical University of Cracow, ul. Podchorazych 2, 30-084 Cracow (Poland); Dufour, P.; Lachapelle, F.-R.; Bergeron, P. [Departement de Physique, Universite de Montreal, C. P. 6128, Succ. Centre-Ville, Montreal, Quebec H3C 3J7 (Canada); Yip, Ching-Wa [Department of Physics and Astronomy, The Johns Hopkins University, 3701 San Martin Drive, Baltimore, MD 21218 (United States); Harris, Hugh C. [United States Naval Observatory, Flagstaff Station, 10391 West Naval Observatory Road, Flagstaff, AZ 86001-8521 (United States); Eisenstein, Daniel J. [Harvard Smithsonian Center for Astrophysics, 60 Garden Street, MS 20, Cambridge, MA 02138 (United States); Althaus, L.; Corsico, A., E-mail: hch@nofs.navy.mil [Facultad de Ciencias Astronomicas y Geofisicas, Paseo del Bosque S/N, (1900) La Plata (Argentina)

    2013-01-15

    We present a new catalog of spectroscopically confirmed white dwarf stars from the Sloan Digital Sky Survey (SDSS) Data Release 7 spectroscopic catalog. We find 20,407 white dwarf spectra, representing 19,712 stars, and provide atmospheric model fits to 14,120 DA and 1011 DB white dwarf spectra from 12,843 and 923 stars, respectively. These numbers represent more than a factor of two increase in the total number of white dwarf stars from the previous SDSS white dwarf catalogs based on DR4 data. Our distribution of subtypes varies from previous catalogs due to our more conservative, manual classifications of each star in our catalog, supplementing our automatic fits. In particular, we find a large number of magnetic white dwarf stars whose small Zeeman splittings mimic increased Stark broadening that would otherwise result in an overestimated log g if fit as a non-magnetic white dwarf. We calculate mean DA and DB masses for our clean, non-magnetic sample and find the DB mean mass is statistically larger than that for the DAs.

  7. This is not Dr. Conn's aldosterone anymore.

    Science.gov (United States)

    Brown, Nancy J

    2011-01-01

    In 1955, Dr. Jerome Conn described a patient with severe hypertension and hypokalemia and an aldosterone-secreting adenoma. The prevalence of hyperaldosteronism is increased among patients with obesity or resistant hypertension. Angiotensin-converting enzyme (ACE) inhibitors and angiotensin receptor blockers reduce the secretion of aldosterone, but with chronic treatment aldosterone concentrations "escape" back to baseline values. Mineralocorticoid receptor (MR) antagonism reduces mortality in patients with heart disease who are already taking an ACE inhibitor and diuretic. In addition to affecting sodium and potassium homeostasis via classical MR-dependent pathways, aldosterone induces inflammation and causes cardiovascular remodeling and renal injury. Some of these effects involve MR-independent pathways. At the same time, ligands other than aldosterone can activate the MR. This paper reviews mechanism(s) for the proinflammatory and profibrotic effects of aldosterone and presents data indicating that endogenous aldosterone, acting at the MR, contributes to many of the pro-inflammatory and pro-fibrotic effects of angiotensin II in vivo.

  8. Carbon stars from LAMOST DR2 data

    CERN Document Server

    Ji, Wei; Liu, Chao; Luo, Ali; Zhao, Gang; Zhang, Bo

    2016-01-01

    In this work, we present the new catalog of carbon stars from the LAMOST DR2 catalog. In total, 894 carbon stars are identified from multiple line indices measured from the stellar spectra. Combining the CN bands in the red end with \\ctwo\\ and other lines, we are able to identify the carbon stars. Moreover, we also classify the carbon stars into spectral sub-types of \\ch, \\CR, and \\cn. These sub-types approximately show distinct features in the multi-dimensional line indices, implying that in the future we can use them to identify carbon stars from larger spectroscopic datasets. Meanwhile, from the line indices space, while the \\cn\\ stars are clearly separated from the others, we find no clear separation between \\CR\\ and \\ch\\ sub-types. The \\CR\\ and \\ch\\ stars seem to smoothly transition from one to another. This may hint that the \\CR\\ and \\ch\\ stars may not be different in their origins but look different in their spectra because of different metallicity. Due to the relatively low spectral resolution and low...

  9. Dr. Rudolph Binion: professor, mentor, psychohistorian.

    Science.gov (United States)

    Szaluta, Jacques

    2015-01-01

    As the title of my paper indicates, Dr. Rudolph Binion was my professor, mentor, and a leading psychohistorian. My paper in memoriam to Rudolph Binion is intended as both a retrospective and an introspective account of my relationship with him, as he had a pivotal influence on me when he was my professor at Columbia University. His help and influence continued after I left graduate school. In my paper I also deal with the enormous stresses of navigating through graduate school, for those students whose goal was to earn the Ph.D. degree. Some examinations were dreaded, For Example The "Examination in Subjects," popularly called the "Oral Exam." The "incubation" period was long indeed, frequently averaging nearly ten years, and it was an ordeal, as the rate of attrition was very high. There is then also the question of "ego strength" and that of "transference" toward the professor. Graduate school is indeed a long and strenuous challenge. I took a seminar in modern French history, a requirement for the Master's degree with Professor Binion, which was consequential for me, as he taught me to be objective in writing history. Professor Binion was a demanding and outstanding teacher.

  10. Invited commentary: the testimony of Dr. Snow.

    Science.gov (United States)

    Vandenbroucke, J P

    2000-07-01

    This paper presents a commentary on the testimony delivered by Dr. John Snow before the British Parliamentary Committee in 1855. It is noted that in Snow's testimony was highlighted his extremely strong belief in germ theory and contagion and his consequent contempt for anything close to the rivaling theory that miasmatic emanations cause disease. Snow's unreasonableness may have been because he already held his germ theory and drinking water convictions before he made his observations. In addition, based on his published books it has become apparent that even his data analysis was guided by such preconceptions. Although Snow's opinion on germ theory created contemporaries, it is noted however, that Snow did not truly sway contemporary opinion, and his theories were not agreed as a complete breakthrough in the way that they are often presented as being in epidemiological textbooks. Overall, the fundamental problem with the miasma and contagion theories at the time was that both failed to answer certain questions, including transmission of diseases, development of diseases, and the issue of temporary carriers of causative germs.

  11. Results from DR and Instrumentation Test Facilities

    CERN Document Server

    Urakawa, Junji

    2005-01-01

    The KEK Accelerator Test Facility (ATF) is a 1.3GeV storage ring capable of producing ultra-low emittance electron beams and has a beam extraction line for ILC R&D. The ATF has proven to be an ideal place for researches with small, stable beams. 2x1010 single bunch and low current 20 bunch-train with 2.8nsec bunch spacing have been extracted to develop Nano-Cavity BPM’s, FONT, Nano Beam Orbit handling (FEATHER), Optical Diffraction Radiation (ODR) monitor, a precision multi-bunch laser-based beam profile monitor and polarized positron beam generation via backward-Compton scattering by the international collaboration. A set of three cavity BPM's is installed in the ATF extraction line on a set of extremely stiff supports. The KEK group installed another set of three BPM's, with their own support mechanism. The full set of 6 will prove extremely useful. In the DR (Damping Ring), we are researching the fast ion instability, micro-wave instability with four sets of damping wiggler and developing pul...

  12. Galactic rotation in Gaia DR1

    CERN Document Server

    Bovy, Jo

    2016-01-01

    The spatial variations of the velocity field of local stars provide direct evidence of Galactic differential rotation. The local divergence, shear, and vorticity of the velocity field---the traditional Oort constants---can be measured based purely on astrometric measurements and in particular depend linearly on proper motion and parallax. I use data for 304,267 main-sequence stars from the Gaia DR1 Tycho-Gaia Astrometric Solution to perform a local, precise measurement of the Oort constants at a typical heliocentric distance of 230 pc. The pattern of proper motions for these stars clearly displays the expected effects from differential rotation. I measure the Oort constants to be: A = 15.3+/-0.4+/- 0.5 (syst.) km/s/kpc, B = -11.9+/-0.4 km/s/kpc, C = -3.2+/-0.4 km/s/kpc and K = -3.3+/-0.6 km/s/kpc, where the systematic uncertainty in A is due to its uncertain correction for the asymmetric drift. These measurements imply local values for the rotational frequency of \\Omega_0 = 27.1+/-0.5+/- 0.5 (syst.) km/s/kpc,...

  13. Dr. Otto "Tiger" Freer: inventor and innovator.

    Science.gov (United States)

    Chittiboina, Prashant; Connor, David E; Nanda, Anil

    2012-08-01

    Every neurosurgeon develops his or her own standard approach to common intracranial pathologies in terms of the order in which different stages are performed and which instruments are used to perform individual tasks. The majority of the basic steps in performing a craniotomy are learned through repetition and practice during residency training. Significant amounts of energy are devoted to mastering technical skills and developing an operative rhythm. What often receives little attention is the historical origin of the instruments that make the work possible. The Freer elevator represents a particularly interesting example. To people unfamiliar with the accomplishments of turn-of-the-century laryngologist Otto "Tiger" Freer, it can be assumed that the name of the instrument in one's hand is simply named for what it can do, that is, to "free" the nasal mucosa from the bony and cartilaginous septum during the transsphenoidal approach. The technique this master surgeon spent his life and career perfecting is now repeated almost daily by skull base neurosurgeons approaching pathologies from the inferior frontal lobe to the foramen magnum. In reviewing his life and work, the authors of this paper discovered an interesting creative process that led to the design of the eponymous instrument. Additionally, they discovered important advances toward the development of the transnasal approach and in our understanding of the anterior skull base. They present a historical perspective on the life and accomplishments of Dr. Freer and the ubiquitous surgical instrument that he invented and popularized.

  14. An interview with Dr Diane Austin

    Directory of Open Access Journals (Sweden)

    Patricia Preston-Roberts

    2011-03-01

    Full Text Available For many years Dr Diane Austin has been developing a very special use of the voice in her practice as a music psychotherapist. The transformational aspects of this approach have been witnessed by colleagues who have heard Diane present at conferences or have read of her work in various publications. There are now further opportunities for colleagues to find out more about her work by reading her recent book or participating in her trainings. On behalf of the editors of the series of interviews for Voices I am delighted to be able to introduce this interview with Diane by one of her colleagues Patricia Preston-Roberts. There is also a linked audio example. In the interview Diane explains how her approach evolved and she identifies some of the key features of the work. She provides some links to the theories underpinning her practice and talks very openly about some issues of countertransference that arise as part of this very powerful and profound way of working. Throughout the interview we are aware how all aspects of the voice have an enormous influence on the development of a warm and trusting therapeutic relationship. From the moment a client enters Diane's consulting room it is clear that her deep and empathic listening ability enables her to make connections to every vocal nuance, whether spoken or sung. As she points out "the whole session" can be viewed "as an improvisation."

  15. Study of HLA-DR synthesis in cultured human keratinocytes.

    Science.gov (United States)

    Wikner, N E; Huff, J C; Norris, D A; Boyce, S T; Cary, M; Kissinger, M; Weston, W L

    1986-11-01

    Within the normal human epidermis only Langerhans and indeterminate cells express HLA-DR. Human keratinocytes (HK), however, may also express HLA-DR in certain disease states characterized by mononuclear cell infiltrates. Previous studies have shown that HK synthesize HLA-DR in response to stimulation by interferon gamma (INF-gamma). The purposes of this study were to define conditions under which cultured HK might express HLA-DR and to compare the HLA-DR synthesis of HK with that of monocytes. HLA-DR expression by HK as determined by indirect immunofluorescence of HK cultures was absent under standard low calcium conditions and remained absent with the addition of calcium, serum, mitogens, and supernatants from Pam-212 cells containing epidermal thymocyte-activating factor. HLA-DR expression in HK was induced by cocultivation with concanavalin A-stimulated peripheral blood mononuclear cells (PBMC), but not unstimulated PBMC. This effect was time-dependent and directly related to the number of PBMC. HLA-DR expression was also induced in a time- and dose-dependent manner by addition of supernatant from stimulated PBMC (SS) or by addition of recombinant INF-gamma but not by addition of interleukin (IL)-1 or IL-2. Induction by either SS or INF-gamma was blocked by an antiserum to INF-gamma. As determined by a semiquantitative immunoprecipitation technique, HLA-DR synthesis by HK was directly related to INF-gamma concentration. The pattern of HLA-DR peptides produced by HK was similar to that of monocytes, but the relative quantity synthesized was far less than that of monocytes.

  16. Induction of Tumor Cell Apoptosis via Fas/DR5

    Institute of Scientific and Technical Information of China (English)

    Wenzhu Li; Shengyu Wang; Caixia Chen; Guohong Zhuang

    2006-01-01

    The apoptosis inducing effects on tumor cell lines MGC803, BEL7402 and HL60 by Fas ligand and anti-human DR5 monoclonal antibodies (anti-DR5 mAb) and the underlying mechanism was studied, Fas/DR5 mRNA was detected by RT-PCR. Cytotoxicity exerted by FasL/anti-DR5 mAb on tumor cell lines was measured by MTT assay and the induced apoptosis was determined by agarose gel electrophoresis. Flow cytometry was employed to analyze the mode of cell death. The mRNA expression of DR5 in MGC803 and BEL7402 cells after giving anti-DR5 mAb was up-regulated compared with control group, while it was down-regulated in HL60 cells in the same condition.The mRNA expression of Fas in HL60 was higher after giving FasL compared with control group, while it was lower in MGC803 and BEL7402. MGC803 and BEL7402 were sensitive to anti-DR5 mAb but partially to FasL,and HL60 was sensitive to FasL but less sensitive to anti-DR5 mAb. Apoptosis induced by Fas ligand and anti-DR5 mAb vary among tumor cell lines. The underlying mechanism may be relevant to Fas/DR5 mRNA expression,which was presented as the release of caspase-8 and Bcl-2.

  17. A non-inheritable maternal Cas9-based multiple-gene editing system in mice.

    Science.gov (United States)

    Sakurai, Takayuki; Kamiyoshi, Akiko; Kawate, Hisaka; Mori, Chie; Watanabe, Satoshi; Tanaka, Megumu; Uetake, Ryuichi; Sato, Masahiro; Shindo, Takayuki

    2016-01-28

    The CRISPR/Cas9 system is capable of editing multiple genes through one-step zygote injection. The preexisting method is largely based on the co-injection of Cas9 DNA (or mRNA) and guide RNAs (gRNAs); however, it is unclear how many genes can be simultaneously edited by this method, and a reliable means to generate transgenic (Tg) animals with multiple gene editing has yet to be developed. Here, we employed non-inheritable maternal Cas9 (maCas9) protein derived from Tg mice with systemic Cas9 overexpression (Cas9 mice). The maCas9 protein in zygotes derived from mating or in vitro fertilization of Tg/+ oocytes and +/+ sperm could successfully edit the target genome. The efficiency of such maCas9-based genome editing was comparable to that of zygote microinjection-based genome editing widely used at present. Furthermore, we demonstrated a novel approach to create "Cas9 transgene-free" gene-modified mice using non-Tg (+/+) zygotes carrying maCas9. The maCas9 protein in mouse zygotes edited nine target loci simultaneously after injection with nine different gRNAs alone. Cas9 mouse-derived zygotes have the potential to facilitate the creation of genetically modified animals carrying the Cas9 transgene, enabling repeatable genome engineering and the production of Cas9 transgene-free mice.

  18. Cas9 in Genetically Modified Food Is Unlikely to Cause Food Allergy.

    Science.gov (United States)

    Nakajima, Osamu; Nishimaki-Mogami, Tomoko; Kondo, Kazunari

    2016-01-01

    Genome editing has undergone rapid development during the last three years. It is anticipated that genetically modified organisms (GMOs) for food purposes will be widely produced using the clustered regularly interspaced short palindromic repeat/Cas9 (CRISPR)/Cas9 system in the near future. However, the Cas9 gene may then enter the genomes of GMOs for food if the breeding process is not strictly managed, which could lead to the Cas9 protein or associated peptides being produced within these organisms. A variety of peptides could theoretically be produced from the Cas9 gene by using open reading frames different from that of Cas9 in the GMOs. In this study, Cas9 and the peptides potentially encoded by Cas9 genes were studied regarding their immunogenicity, in terms of the digestibility of Cas9 and the homology of the peptides to food allergens. First, the digestibility and thermal stability of Cas9 were studied. Digestibility was tested with natural or heat-denatured Cas9 in simulated gastric fluid in vitro. The two types of Cas9 were digested rapidly. Cas9 was also gradually degraded during heat treatment. Second, the peptides potentially encoded by Cas9 genes were examined for their homology to food allergens. Specifically, an 8-mer exact match search and a sliding 80-mer window search were performed using allergen databases. One of the peptides was found to have homology with a food allergen.

  19. Dr. Steve Thompson, Chief Executive, The Royal Society of New Zealand

    CERN Multimedia

    Maximilien Brice

    2002-01-01

    L. to r.: Dr Austin Ball, Deputy Technical Coordinator, CMS experiment; Dr Roland Horisberger, Paul Scherrer Institute and CERN, CMS experiment; Dr Steve Thompson, Chief Executive, The Royal Society of New Zealand; Dr Michel Della Negra, Spokesman, CMS experiment and Dr Alick Macpherson, Paul Scherrer Institute and CERN, CMS experiment, in the CMS Silicon Tracker assembly hall.

  20. Complementary Information Derived from CRISPR Cas9 Mediated Gene Deletion and Suppression. | Office of Cancer Genomics

    Science.gov (United States)

    CRISPR-Cas9 provides the means to perform genome editing and facilitates loss-of-function screens. However, we and others demonstrated that expression of the Cas9 endonuclease induces a gene-independent response that correlates with the number of target sequences in the genome. An alternative approach to suppressing gene expression is to block transcription using a catalytically inactive Cas9 (dCas9). Here we directly compare genome editing by CRISPR-Cas9 (cutting, CRISPRc) and gene suppression using KRAB-dCas9 (CRISPRi) in loss-of-function screens to identify cell essential genes.

  1. Spectroscopic studies of three Cepheids with high positive pulsation period increments: SZ Cas, BY Cas, and RU Sct

    Science.gov (United States)

    Usenko, I. A.; Klochkova, V. G.

    2015-07-01

    Three high-resolution spectra have been taken at different times with the 6-m SAO RAS telescope (LYNX and PFES spectrographs) for three Cepheids exhibiting high positive period increments: the small-amplitude (DCEPS) SZ Cas and BY Cas and the classical (DCEP) RU Sct. SZ Cas and RU Sct are members of the Galactic open clusters χ and h Per and Trump 35, respectively. Analysis of the spectra has shown that the interstellar Na I D1 and D2 lines in all objects are considerably stronger than the atmospheric ones and are redshifted in SZ Cas and BY Cas and blushifted in RU Sct. The core of the H α absorption line in BY Cas has an asymmetric knifelike shape, while RU Sct exhibits an intense emission in the blue wing of this line. Such phenomena are observed in long-period Cepheids and bright hypergiants with an extended envelope. In this case, the strong Mg Ib 5183.62 Å and Ba II 5853.67, 6141.713, and 6496.90 Å lines with low χlow in SZ Cas and RU Sct also show characteristic knifelike profiles with an asymmetry in the red region, while the Ba II 4934.095 Å line shows similar profiles in the blue one. The absorption lines of neutral atoms and singly ionized metals with different lowerlevel excitation potentials exhibit different degrees of asymmetry: from a pronounced one with secondary components in BY Cas (similar to those in the small-amplitude Cepheid BG Cru pulsating in the first overtone and having an envelope) to its insignificance or virtual absence in SZ Cas and RU Sct. Analysis of the secular changes in mean T eff determined from photometric color indices and spectra over the last 55 years for these stars has revealed periodic fluctuations of 200 K for SZ Cas and BY Cas and 500 K for RU Sct. For SZ Cas and RU Sct, T eff determined in some years from some color indices show much lower values, which together with the temperature fluctuations can be associated with mass loss and dust formation. Based on these facts, we hypothesize the existence of

  2. Polycultural psychology.

    Science.gov (United States)

    Morris, Michael W; Chiu, Chi-yue; Liu, Zhi

    2015-01-01

    We review limitations of the traditional paradigm for cultural research and propose an alternative framework, polyculturalism. Polyculturalism assumes that individuals' relationships to cultures are not categorical but rather are partial and plural; it also assumes that cultural traditions are not independent, sui generis lineages but rather are interacting systems. Individuals take influences from multiple cultures and thereby become conduits through which cultures can affect each other. Past literatures on the influence of multiple cultural identities and cultural knowledge legacies can be better understood within a polyculturalist rubric. Likewise, the concept elucidates how cultures are changed by contact with other cultures, enabling richer psychological theories of intercultural influence. Different scientific paradigms about culture imply different ideologies and policies; polyculturalism's implied policy of interculturalism provides a valuable complement to the traditional policy frames of multiculturalism and colorblindness.

  3. Pyo-pneumothorax tuberculeux: à propos de 18 cas

    Science.gov (United States)

    Hicham, Souhi; Hanane, El Ouazzani; Hicham, Janah; Ismaïl, Rhorfi; Ahmed, Abid

    2016-01-01

    Le pyo-pneumothorax tuberculeux est une complication rare mais grave de la tuberculose pulmonaire évolutive. Nous rapportons une série de 18 cas de pyo-pneumothorax tuberculeux colligés au service de Pneumo-Phtisiologie de l'Hôpital Militaire d'Instruction Mohammed V de Rabat entre janvier 2005 et décembre 2009. Il s'agit de 15 hommes et 3 femmes d’âge moyen de 35 ans ±7 ans. 4 patients étaient diabétiques. Le tabagisme était retrouvé chez 9 cas. Le pyo-pneumothorax était du coté droit dans 13 cas. La radiographie thoracique avait montré des lésions cavitaires chez 15 patients et des lésions étendues et bilatérales chez 8 cas. La recherche de BK dans le liquide de tubage gastrique était positive chez 16 cas. Un drainage thoracique associé à un traitement antituberculeux selon le régime 2SRHZ/7RH et une kinésithérapie respiratoire ont été instaurés chez tous les cas. La durée moyenne de drainage pleural était de 4 semaines. Chez 3 cas on avait noté la persistance de la suppuration pleurale ayant nécessité une toilette pleurale sous thoracoscopie avec pleurectomie et exérèse pulmonaire limitée emportant la lésion parenchymateuse tuberculeuse et la persistance d'une volumineuse poche pleurale avec trouble ventilatoire restrictif ayant nécessité une décortication pleurale chirurgicale chez deux cas. L’évolution était favorable avec pachypleurite séquellaire minime chez le reste des cas. Le pyo-pneumothorax tuberculeux est une forme grave, qui est souvent en rapport avec une tuberculose cavitaire active. L’évolution est généralement trainante malgré le traitement antituberculeux et le drainage thoracique, d'où la nécessité d'un diagnostic et un traitement précoce de toute forme de tuberculose. PMID:27583090

  4. Enrico Fermi Awards Ceremony for Dr. Mildred S. Dresselhaus and Dr. Burton Richter, May 2012 (Presentations, including remarks by Energy Secretary, Dr. Steven Chu)

    Energy Technology Data Exchange (ETDEWEB)

    Chu, Steven (U.S. Energy Secretary)

    2012-05-07

    The Fermi Award is a Presidential award and is one of the oldest and most prestigious science and technology honors bestowed by the U.S. Government. On May 7, 2012 it was conferred upon two exceptional scientists: Dr. Mildred Dresselhaus, 'for her scientific leadership, her major contributions to science and energy policy, her selfless work in science education and the advancement of diversity in the scientific workplace, and her highly original and impactful research,' and Dr. Burton Richter, 'for the breadth of his influence in the multiple disciplines of accelerator physics and particle physics, his profound scientific discoveries, his visionary leadership as SLAC Director, his leadership of science, and his notable contributions in energy and public policy.' Dr. John Holder, Director of the White House Office of Science and Technology Policy, opened the ceremony, and Dr. Bill Brinkman, Director of DOE's Office of Science introduced the main speaker, Dr. Steven Chu, U.S. Energy Secretary.

  5. SD-CAS: Spin Dynamics by Computer Algebra System.

    Science.gov (United States)

    Filip, Xenia; Filip, Claudiu

    2010-11-01

    A computer algebra tool for describing the Liouville-space quantum evolution of nuclear 1/2-spins is introduced and implemented within a computational framework named Spin Dynamics by Computer Algebra System (SD-CAS). A distinctive feature compared with numerical and previous computer algebra approaches to solving spin dynamics problems results from the fact that no matrix representation for spin operators is used in SD-CAS, which determines a full symbolic character to the performed computations. Spin correlations are stored in SD-CAS as four-entry nested lists of which size increases linearly with the number of spins into the system and are easily mapped into analytical expressions in terms of spin operator products. For the so defined SD-CAS spin correlations a set of specialized functions and procedures is introduced that are essential for implementing basic spin algebra operations, such as the spin operator products, commutators, and scalar products. They provide results in an abstract algebraic form: specific procedures to quantitatively evaluate such symbolic expressions with respect to the involved spin interaction parameters and experimental conditions are also discussed. Although the main focus in the present work is on laying the foundation for spin dynamics symbolic computation in NMR based on a non-matrix formalism, practical aspects are also considered throughout the theoretical development process. In particular, specific SD-CAS routines have been implemented using the YACAS computer algebra package (http://yacas.sourceforge.net), and their functionality was demonstrated on a few illustrative examples.

  6. Targeting Hepatitis B Virus With CRISPR/Cas9

    Directory of Open Access Journals (Sweden)

    Christoph Seeger

    2014-01-01

    Full Text Available Hepatitis B virus persistence in infected hepatocytes is due to the presence of covalently closed circular DNA (cccDNA, the template for the transcription of viral RNAs. Antiviral therapies with nucleoside analogues inhibit replication of HBV DNA in capsids present in the cytoplasm of infected cells, but do not reduce or destroy nuclear cccDNA. To investigate whether cccDNA derived from infectious HBV could be directly targeted for destruction, we used the CRISPR/Cas9 system in HepG2 cells expressing the HBV receptor sodium taurocholate cotransporting polypeptide (NTCP. We tested different HBV-specific guide RNAs and demonstrated that they could inhibit HBV infections up to eightfold. Inhibition was due to mutations and deletions in cccDNA similar to those observed with chromosomal DNA cleaved by Cas9 and repaired by nonhomologous end joining (NHEJ. Interferon alpha (IFN-α did not have a measurable effect on the antiviral activity of the CRISPR/Cas9 system, suggesting that Cas9 and NHEJ activities are not affected by induction of an innate immune response with the cytokine. Taken together, our results demonstrated that Cas9 can be recruited to cccDNA, opening the possibility for the development of future antiviral strategies aimed at targeting cccDNA for endonucleolytic cleavage with small molecules.

  7. Repurposing CRISPR/Cas9 for in situ functional assays.

    Science.gov (United States)

    Malina, Abba; Mills, John R; Cencic, Regina; Yan, Yifei; Fraser, James; Schippers, Laura M; Paquet, Marilène; Dostie, Josée; Pelletier, Jerry

    2013-12-01

    RNAi combined with next-generation sequencing has proven to be a powerful and cost-effective genetic screening platform in mammalian cells. Still, this technology has its limitations and is incompatible with in situ mutagenesis screens on a genome-wide scale. Using p53 as a proof-of-principle target, we readapted the CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 (CRISPR associated 9) genome-editing system to demonstrate the feasibility of this methodology for targeted gene disruption positive selection assays. By using novel "all-in-one" lentiviral and retroviral delivery vectors heterologously expressing both a codon-optimized Cas9 and its synthetic guide RNA (sgRNA), we show robust selection for the CRISPR-modified Trp53 locus following drug treatment. Furthermore, by linking Cas9 expression to GFP fluorescence, we use an "all-in-one" system to track disrupted Trp53 in chemoresistant lymphomas in the Eμ-myc mouse model. Deep sequencing analysis of the tumor-derived endogenous Cas9-modified Trp53 locus revealed a wide spectrum of mutants that were enriched with seemingly limited off-target effects. Taken together, these results establish Cas9 genome editing as a powerful and practical approach for positive in situ genetic screens.

  8. War, plague and exploitation in DR Congo

    Directory of Open Access Journals (Sweden)

    Dimčevska Antoaneta K.

    2007-01-01

    Full Text Available Late in autumn 2006 one of the headlines in world media was the first democratic elections in DR Congo. They took place after 30 years of Mobutu Sese Seko’s dictatorship and bloody civil wars in the period 1996-2002. These conflicts, which took approximately 4 million human lives, are called "The First African World War". Elections were held but they did not guarantee the end of trouble for the divided and tormented people in the northeast of Congo, the real scene of bloodshed. The area is still turbulent because it abounds in mineral wealth - gold, diamonds and raw materials for nuclear technology. For a whole decade, unscrupulous actors of the African crisis were fighting there, for illegal profits (achievable in the chaos of bloodshed rather than for democracy, defense of tribal interests, security, etc. as they claimed. In the mines of Eastern Congo unprecedented exploitation of people is still going on, especially of children, victims of conflicts, who suffer in great numbers from violence, starvation and diseases. These slaves of the crisis make local "warlords" and their mentors rich. The looting of the mines has stabilized the crisis because it makes possible enormous accumulation of wealth among armed decision-makers - which also includes availability of countless slaves-miners who have lost everything except their bare lives. Eastern Congo is, however, one of world’s three old focuses of plague; wild exploitation of ores in the area of this endemic disease has activated a sleepy focus and added pneumonic plague to the burdens suffered by the population of the rich but ill-fated region. This was to be expected because endemic plague in the gold-bearing evil circumstances impedes safe mining - and this will be the crucial challenge in the future of Congo. This article is an anthropological outline of the area where gold, plague, weapons and incomparable suffering of people merge together just because of cynic greed producing abuse

  9. The Dialectic Psychology perspectives

    OpenAIRE

    Oliveros M., Ricardo; Facultad de Psicología, Universidad Nacional Mayor de San Marcos, Lima, Perú

    2014-01-01

    The present paper exposes the dialectic psychology perspectives in the twentieth first century Peru. We ponder about the dialectics psychology denomination, connecting them to other denominations used in the materialist psychology trend. We analyze the relations between dialectics psychology and social neuroscience, delimiting both the psychological sciences field and the neuroscience field. We develop issues from the emancipator project of dialectics psychology, precising personal developmen...

  10. Evolutionary developmental psychology

    National Research Council Canada - National Science Library

    King, Ashley C; Bjorklund, David F

    2010-01-01

    The field of evolutionary developmental psychology can potentially broaden the horizons of mainstream evolutionary psychology by combining the principles of Darwinian evolution by natural selection...

  11. First period investigation and light-curve study of the eclipsing contact binary V776 Cas

    Science.gov (United States)

    Noori, Hamid Reza; Abedi, Abbas

    2017-10-01

    CCD photometry of the eclipsing binary system V776 Cas was carried out through Johnson-Cousins UBVR filters in Dr. Mojtahedi observatory of Birjand University during August and September 2014. The physical and geometrical parameters of the system were obtained by analyzing the light curves. Radial velocity data were used to determine the absolute parameters. Five new times of minimum light were found. The O-C diagram was plotted relying on these times and the previously reported times of minimum. New ephemeris of the system, the rate of orbital period variations and the mass transfer rate of the system were derived from the O-C diagram analysis. A periodic variation of about 8 years was found from the O-C diagram analysis. At first light-time effect was considered. Although the results confirmed the probability of the presence of third body, Applegate mechanism was checked as the reason for this periodic variation and this mechanism is also in agreement with the observations.

  12. Psychology Ethics in Introductory Psychology Textbooks

    Science.gov (United States)

    Zucchero, Renee' A.

    2011-01-01

    Previous research revealed that introductory psychology textbooks included limited information about psychology ethics. This study reviewed 48 current introductory psychology textbooks for research and other APA ethics content. These textbooks included slightly more total ethics content and were more thorough in their review of research ethics…

  13. Psychology Ethics in Introductory Psychology Textbooks

    Science.gov (United States)

    Zucchero, Renee' A.

    2011-01-01

    Previous research revealed that introductory psychology textbooks included limited information about psychology ethics. This study reviewed 48 current introductory psychology textbooks for research and other APA ethics content. These textbooks included slightly more total ethics content and were more thorough in their review of research ethics…

  14. Dr. George Koob: "Alcohol Use Disorders Are a Major Problem..."

    Science.gov (United States)

    ... please turn JavaScript on. Feature: Rethinking Drinking Dr. George Koob: "Alcohol use disorders are a major problem …" Past Issues / Spring 2014 Table of Contents George Koob, Ph.D., is Director of the NIH's ...

  15. Dr. Omalu Talks Childhood, Concussions, and CTE | Poster

    Science.gov (United States)

    Dr. Bennet Omalu, the famed forensic pathologist who discovered Chronic Traumatic Encephalopathy (CTE), recently spoke at NCI at Frederick about his upbringing as well as the trials he faced while working to educate the NFL about CTE.

  16. Three Typical Dermatological Cases Treated by Dr. Li Yueping

    Institute of Scientific and Technical Information of China (English)

    Xie Shaoqiong

    2005-01-01

    @@ Dr. Li Yueping has been engaged in dermatological clinic for more than 40 years, accumulating rich experience in treating dermatological diseases. The following is three typical cases she gave successful treatment.

  17. Dr Abraham Pais, 82, physicist and science historian dies

    CERN Multimedia

    Glanz, J

    2000-01-01

    Obituary of Dr. Pais who died of heart failure in Copenhagen. He helped build the conceptual foundations of particle physics and then became a science historian, writing a critically acclaimed biography of Einstein (1 pg).

  18. Grantee Spotlight: Dr. Kolawole Okuyemi - Improving Cervical Cancer Screening Attitudes

    Science.gov (United States)

    Dr. Kolawole Okuyumi is studying cervical cancer screening attitudes and behaviors of African immigrants and refugees in Minnesota, and introducing “cancer” and “cervix” to their everyday vocabulary.

  19. Adipose tissue resistin gene expression in DIO and DR rats

    Institute of Scientific and Technical Information of China (English)

    Yuanyuan Zhao; Yuhui Ni; Xirong Guo; Haixia Gong; Xia Chi; Ronghua Chen

    2006-01-01

    Objective: To investigate the expression of resistin gene in diet-induced obesity (DIO) and diet resistance (DR)rats. Methods: DIO and DR models were prepared with male SD rats after 6 weeks feeding by a diet of relatively high fat, sucrose, and caloric content (HE diet). Body-weight, fat mass, and the concentration of serum insulin were measured, and the expression of resistin and Peroxisome proliferator-activated receptory-γ(PPAR-γ) gene in whit adipose tissue (WAT) was also detected by RT-PCR. Results: ①Body weight, fat mass and the concentration of serum insulin were significantly increased in DIO rats and decreased in DR rats. ② The expression of resistin and PPARγ gene was upregulated in DIO group and supressed in DR group, but the expression of resistin was not detectable in all samples within three groups. Conclusion: Resistin may serve as a link between obesity and insulin resistance, but the individual difference is enormous.

  20. Glaucoma: Eye-to-Eye with Dr. Rachel Bishop

    Science.gov (United States)

    ... of this page please turn JavaScript on. Feature: Glaucoma Eye-to-Eye with Dr. Rachel Bishop Past ... nerves are pale and cupped—signs of advanced glaucoma. Yet the patient wasn't aware of any ...

  1. Study of Eclipsing Binary and Multiple Systems in OB Associations IV: Cas OB6 Member DN Cas

    CERN Document Server

    Bakis, V; Bilir, S; Eker, Z

    2016-01-01

    An early-type, massive, short-period ($P_{\\rm orb}=2^{\\rm d}.310951$) eclipsing spectroscopic binary DN Cas has been re-visited with new spectral and photometric data. The masses and radii of the components have been obtained as $M_1=19.04\\pm0.07 M_\\odot$, $M_2=13.73\\pm 0.05M_\\odot$ and $R_1=7.22\\pm0.06 R_\\odot$, $R_2=5.79\\pm0.06R_\\odot$, respectively. Both components present synchronous rotation ($V_{\\rm rot1}=160\\,$km s$^{-1}$, $V_{\\rm rot2}=130\\,$km s$^{-1}$) with their orbit. Orbital period analysis yielded a physically bound additional component in the system with a minimum mass of $M_3=0.88 M_\\odot$ orbiting in an eccentric orbit ($e=0.37\\pm0.2$) with an orbital period of $P_{12}=42\\pm9$ yrs. High precision absolute parameters of the system allowed us to derive a distance to DN Cas as 1.7$\\pm$0.2 kpc which locates the system within the borders of the Cas OB6 association (d=1.8 kpc; Mel'nik & Dambis, 2009). The space velocities and the age of DN Cas are in agreement with those of Cas OB6. The age of ...

  2. CRISPR-Cas9: A Revolutionary Tool for Cancer Modelling

    Directory of Open Access Journals (Sweden)

    Raul Torres-Ruiz

    2015-09-01

    Full Text Available The cancer-modelling field is now experiencing a conversion with the recent emergence of the RNA-programmable CRISPR-Cas9 system, a flexible methodology to produce essentially any desired modification in the genome. Cancer is a multistep process that involves many genetic mutations and other genome rearrangements. Despite their importance, it is difficult to recapitulate the degree of genetic complexity found in patient tumors. The CRISPR-Cas9 system for genome editing has been proven as a robust technology that makes it possible to generate cellular and animal models that recapitulate those cooperative alterations rapidly and at low cost. In this review, we will discuss the innovative applications of the CRISPR-Cas9 system to generate new models, providing a new way to interrogate the development and progression of cancers.

  3. Genome engineering using CRISPR-Cas9 system.

    Science.gov (United States)

    Cong, Le; Zhang, Feng

    2015-01-01

    The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas9 system is an adaptive immune system that exists in a variety of microbes. It could be engineered to function in eukaryotic cells as a fast, low-cost, efficient, and scalable tool for manipulating genomic sequences. In this chapter, detailed protocols are described for harnessing the CRISPR-Cas9 system from Streptococcus pyogenes to enable RNA-guided genome engineering applications in mammalian cells. We present all relevant methods including the initial site selection, molecular cloning, delivery of guide RNAs (gRNAs) and Cas9 into mammalian cells, verification of target cleavage, and assays for detecting genomic modification including indels and homologous recombination. These tools provide researchers with new instruments that accelerate both forward and reverse genetics efforts.

  4. Harnessing CRISPR-Cas systems for bacterial genome editing.

    Science.gov (United States)

    Selle, Kurt; Barrangou, Rodolphe

    2015-04-01

    Manipulation of genomic sequences facilitates the identification and characterization of key genetic determinants in the investigation of biological processes. Genome editing via clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated (Cas) constitutes a next-generation method for programmable and high-throughput functional genomics. CRISPR-Cas systems are readily reprogrammed to induce sequence-specific DNA breaks at target loci, resulting in fixed mutations via host-dependent DNA repair mechanisms. Although bacterial genome editing is a relatively unexplored and underrepresented application of CRISPR-Cas systems, recent studies provide valuable insights for the widespread future implementation of this technology. This review summarizes recent progress in bacterial genome editing and identifies fundamental genetic and phenotypic outcomes of CRISPR targeting in bacteria, in the context of tool development, genome homeostasis, and DNA repair.

  5. Genome Editing in Human Cells Using CRISPR/Cas Nucleases.

    Science.gov (United States)

    Wyvekens, Nicolas; Tsai, Shengdar Q; Joung, J Keith

    2015-10-01

    The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas) system has been broadly adopted for highly efficient genome editing in a variety of model organisms and human cell types. Unlike previous genome editing technologies such as zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs), CRISPR/Cas technology does not require complex protein engineering and can be utilized by any researcher proficient in basic molecular biology and cell culture techniques. This unit describes protocols for design and cloning of vectors expressing single or multiplex gRNAs, for transient transfection of human cell lines, and for quantitation of mutation frequencies by T7 endonuclease I assay. These protocols also include guidance for using two improvements that increase the specificity of CRISPR/Cas nucleases: truncated gRNAs and dimeric RNA-guided FokI nucleases.

  6. GENOME EDITING IN HUMAN CELLS USING CRISPR/CAS NUCLEASES

    Science.gov (United States)

    Wyvekens, Nicolas; Tsai, Shengdar; Joung, J. Keith

    2016-01-01

    The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas) system has been broadly adopted for highly efficient genome editing in a variety of model organisms and human cell types. Unlike previous genome editing technologies such as Zinc Finger Nucleases (ZFNs) and Transcription Activator-Like Effector Nucleases (TALENs), the CRISPR/Cas technology does not require complex protein engineering and can be utilized by any researcher proficient in basic molecular biology and cell culture techniques. Here we describe protocols for design and cloning of vectors expressing single or multiplex gRNAs, for transient transfection of human cell lines, and for quantitation of mutation frequencies by T7 Endonuclease I assay. These protocols also include guidance for using two improvements that increase the specificity of CRISPR/Cas nucleases: truncated gRNAs and dimeric RNA-guided FokI nucleases. PMID:26423589

  7. CRISPR-Cas adaptation: insights into the mechanism of action.

    Science.gov (United States)

    Amitai, Gil; Sorek, Rotem

    2016-02-01

    Since the first demonstration that CRISPR-Cas systems provide bacteria and archaea with adaptive immunity against phages and plasmids, numerous studies have yielded key insights into the molecular mechanisms governing how these systems attack and degrade foreign DNA. However, the molecular mechanisms underlying the adaptation stage, in which new immunological memory is formed, have until recently represented a major unresolved question. In this Progress article, we discuss recent discoveries that have shown both how foreign DNA is identified by the CRISPR-Cas adaptation machinery and the molecular basis for its integration into the chromosome to form an immunological memory. Furthermore, we describe the roles of each of the specific CRISPR-Cas components that are involved in memory formation, and consider current models for their evolutionary origin.

  8. Exploiting CRISPR/Cas: Interference Mechanisms and Applications

    Directory of Open Access Journals (Sweden)

    André Plagens

    2013-07-01

    Full Text Available The discovery of biological concepts can often provide a framework for the development of novel molecular tools, which can help us to further understand and manipulate life. One recent example is the elucidation of the prokaryotic adaptive immune system, clustered regularly interspaced short palindromic repeats (CRISPR/CRISPR-associated (Cas that protects bacteria and archaea against viruses or conjugative plasmids. The immunity is based on small RNA molecules that are incorporated into versatile multi-domain proteins or protein complexes and specifically target viral nucleic acids via base complementarity. CRISPR/Cas interference machines are utilized to develop novel genome editing tools for different organisms. Here, we will review the latest progress in the elucidation and application of prokaryotic CRISPR/Cas systems and discuss possible future approaches to exploit the potential of these interference machineries.

  9. Exploiting CRISPR/Cas: Interference Mechanisms and Applications

    Science.gov (United States)

    Richter, Hagen; Randau, Lennart; Plagens, André

    2013-01-01

    The discovery of biological concepts can often provide a framework for the development of novel molecular tools, which can help us to further understand and manipulate life. One recent example is the elucidation of the prokaryotic adaptive immune system, clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) that protects bacteria and archaea against viruses or conjugative plasmids. The immunity is based on small RNA molecules that are incorporated into versatile multi-domain proteins or protein complexes and specifically target viral nucleic acids via base complementarity. CRISPR/Cas interference machines are utilized to develop novel genome editing tools for different organisms. Here, we will review the latest progress in the elucidation and application of prokaryotic CRISPR/Cas systems and discuss possible future approaches to exploit the potential of these interference machineries. PMID:23857052

  10. CRISPR-Cas9: A Revolutionary Tool for Cancer Modelling.

    Science.gov (United States)

    Torres-Ruiz, Raul; Rodriguez-Perales, Sandra

    2015-09-14

    The cancer-modelling field is now experiencing a conversion with the recent emergence of the RNA-programmable CRISPR-Cas9 system, a flexible methodology to produce essentially any desired modification in the genome. Cancer is a multistep process that involves many genetic mutations and other genome rearrangements. Despite their importance, it is difficult to recapitulate the degree of genetic complexity found in patient tumors. The CRISPR-Cas9 system for genome editing has been proven as a robust technology that makes it possible to generate cellular and animal models that recapitulate those cooperative alterations rapidly and at low cost. In this review, we will discuss the innovative applications of the CRISPR-Cas9 system to generate new models, providing a new way to interrogate the development and progression of cancers.

  11. Den som dræber spændingen

    DEFF Research Database (Denmark)

    Agger, Gunhild

    2011-01-01

    TV 2's stort lancerede krimithriller ’Den som dræber’ er klicheernes holdeplads. Det gør det til en kedelig oplevelse at tænde for TV 2 søndag aften kl. 20. TV 2 har udfordret DR på sendetidspunktet, men har for lidt at byde på. Der er tale om ren rutine. Ingen af genrens centrale elementer glimrer...

  12. A periodontal case report by Dr. S.L. Clemens.

    Science.gov (United States)

    Maloney, W J

    2010-07-01

    Mark Twain provides a humorous and insightful look into the origins of periodontal therapy and anesthesia in "Happy Memories of the Dental Chair". The main character of this story is Dr. John Riggs, the father of periodontics. Dr. Horace Wells, a pioneer in the field of anesthesia, also appears in the story. Twain presents his autobiographical experiences with dentistry in his literary work in a form that can be seen to be similar to a case report in a professional dental journal.

  13. Dr. David Syz, State Secretary for Economic Affairs, Switzerland

    CERN Document Server

    Maximilien Brice

    2003-01-01

    Dr. David Syz, State Secretary for Economic Affairs, Switzerland is seen here (seventh from right) visiting the assembly hall for the ATLAS experiment during his recent visit to CERN. To his right is Dr. Peter Jenni (blue shirt), spokesperson for the ATLAS Collaboration. The horizontal metal cylinder behind the group is one of the eight vacuum vessels for the superconducting coils of the ATLAS barrel toroid magnet system.

  14. The research contributions of Dr. Paul Van Deusen

    Science.gov (United States)

    Thomas B. Lynch; Francis A. Roesch; John Paul McTague; Jeffrey H. Gove; Gregory A. Reams; Aaron R. Weiskittel

    2015-01-01

    Dr. Paul Van Deusen’s recent passing concluded a rich 30+-year research career dedicated to development and implementation of quantitative methods for forestry and natural resources. Since the early part of his career as a biometrician with the USDA Forest Service Southern Research Station in the 1980s-1990s and continuing with his later employment at NCASI, Dr. Van...

  15. Portrait of Dr. Von Braun with Walt Disney, 1954.

    Science.gov (United States)

    1954-01-01

    Marshall Center Director Dr. Wernher Von Braun is pictured with Walt Disney during a visit to the Marshall Space Flight Center in 1954. In the 1950s, Dr. Von Braun while working in California on the Saturn project, also worked with Disney studios as a technical director in making three films about Space Exploration for television. Disney's tour of Marshall in 1965 was Von Braun's hope for a renewed public interest in the future of the Space Program at NASA.

  16. Dr. Peter Emil Becker and the Third Reich.

    Science.gov (United States)

    Hill, Frank

    2013-08-01

    In 1985 the physician after whom Becker Muscular Dystrophy is named, German neurologist Dr. Peter Emil Becker (1908-2000), published an autobiographical article in the American Journal of Medical Genetics in which he disavowed any association with the Nazi Party. A closer look at the evidence, however, suggests otherwise. Review of war records and related sources raise concern for Dr. Becker's affiliation with the Nazi Party and his contributions to its ideology.

  17. Rapid and tunable method to temporally control gene editing based on conditional Cas9 stabilization.

    Science.gov (United States)

    Senturk, Serif; Shirole, Nitin H; Nowak, Dawid G; Corbo, Vincenzo; Pal, Debjani; Vaughan, Alexander; Tuveson, David A; Trotman, Lloyd C; Kinney, Justin B; Sordella, Raffaella

    2017-02-22

    The CRISPR/Cas9 system is a powerful tool for studying gene function. Here, we describe a method that allows temporal control of CRISPR/Cas9 activity based on conditional Cas9 destabilization. We demonstrate that fusing an FKBP12-derived destabilizing domain to Cas9 (DD-Cas9) enables conditional Cas9 expression and temporal control of gene editing in the presence of an FKBP12 synthetic ligand. This system can be easily adapted to co-express, from the same promoter, DD-Cas9 with any other gene of interest without co-modulation of the latter. In particular, when co-expressed with inducible Cre-ER(T2), our system enables parallel, independent manipulation of alleles targeted by Cas9 and traditional recombinase with single-cell specificity. We anticipate this platform will be used for the systematic characterization and identification of essential genes, as well as the investigation of the interactions between functional genes.

  18. CAS – A Journey Has Begun in Aotearoa New Zealand

    Directory of Open Access Journals (Sweden)

    Derek Smith

    2008-08-01

    Full Text Available This paper explores a journey through hand-held technology changes in mathematics teaching and learning and raises questions we as mathematics educators should be considering in the shorter and longer term. New Zealand is embarking on a Computer Algebraic Systems (CAS Pilot Programme in secondary school mathematics. The Ministry of Education and the New Zealand Qualifications Authority have selected secondary schools to be part of a pilot programme in the use of CAS technology in mathematics classes. The aim of the pilot programme is to improve teaching and learning of mathematics through the use of this technology. Six schools in 2005 used CAS technology with Year 9 (13-14 year olds students and, an additional 16 schools joined the programme in 2006. The pilot is planned to continue with an increasing number of schools in subsequent years. By the time students in the pilot schools reach Years 11, 12 and 13, alternative external assessments using the CAS technology will be available. Professional development support and assistance in obtaining and using the technology will be provided to the pilot schools. The project's emphasis in 2005 was on the Geometry and Algebra strands; the Statistics strand was added in 2006. By 2010 the first cohort of project programme students will have been through their secondary mathematics education via a CAS environment. New Zealand teachers have only a finite time to get into CAS technology and integrate it into their teaching practice. This paper discusses a research project based on a mathematics department professional development that is linked to the pilot.

  19. Dr. John H. Hopps Jr. Defense Research Scholars Program

    Science.gov (United States)

    2014-12-16

    Aparecio Peggins (Psychology) Past Research Experience • Attachment Styles in Romantic Relationships, University of New Hampshire (Summer 2011...Psychology Neurobiology of Circadian Entrainment Aparecio Peggins Duane Cooper Professor and Chair of Mathematics Mathematics student and

  20. DR-current intensity variations during substorm development

    Science.gov (United States)

    Zaitseva, S. A.; Drobinina, T. A.; Pudovkin, M. I.

    2003-04-01

    According to the concept dominated by the last time, the intensification of the DR-current takes place during the polar substorm development. In the recent papers Iyemori and Rao claim that geomagnetic storms and substorms are independent processes, and SYM-index (essentially the same as Dst-index) tends to decay after the onset of substorms. In this paper we tried to clear up the cause of this contradiction by studying the connection of ring current development with polar substorms basing on the new data: ASY-, SYM-indices describing asymmetric and symmetric parts of DR-current correspondingly and AL-index as a measure of polar disturbances. It is shown that the hourly-mean SYM- and ASY-indices correlate with AL-index (r=0.63 and 0.69 correspondingly), and the connection between these values becomes worse for strong storms. Besides, at the substorm expansive phase onset, the energy can put mainly to the asymmetric part rather than into symmetric part of DR-current. Empirical Q-index based on solar wind parameters describes the energy input into DR-current; Q correlates rather well with SYM, ASY and AL-indices. Thus the intensifications of polar disturbances and DR-current take place simultaneously and have the same source.At the same time, the proportion in which the solar wind energy is distributed between the DR-current, polar ionosphere and magnetotail depends on the Dst variation phase.

  1. Targeted genome modifications in soybean with CRISPR/Cas9

    OpenAIRE

    Jacobs, Thomas; LaFayette, Peter R.; Schmitz, Robert J.; Parrott, Wayne A.

    2015-01-01

    Background The ability to selectively alter genomic DNA sequences in vivo is a powerful tool for basic and applied research. The CRISPR/Cas9 system precisely mutates DNA sequences in a number of organisms. Here, the CRISPR/Cas9 system is shown to be effective in soybean by knocking-out a green fluorescent protein (GFP) transgene and modifying nine endogenous loci. Results Targeted DNA mutations were detected in 95% of 88 hairy-root transgenic events analyzed. Bi-allelic mutations were detecte...

  2. Applications of CRISPR-Cas9 mediated genome engineering

    Institute of Scientific and Technical Information of China (English)

    Xiao Yang

    2015-01-01

    Targeted mutagenesis based on homologous recombination has been a powerful tool for understanding the mechanisms underlying development, normal physiology, and disease. A recent breakthrough in genome engineering technology based on the class of RNA-guided endonucleases, such as clustered regularly interspaced short palindromic repeats (CRISPR)-associated Cas9, is further revolutionizing biology and medical studies. The simplicity of the CRISPR-Cas9 system has enabled its widespread applications in generating germline animal models, somatic genome engineering, and functional genomic screening and in treating genetic and infectious diseases. This technology will likely be used in all fields of biomedicine, ranging from basic research to human gene therapy.

  3. Expanding the Biologist's Toolkit with CRISPR-Cas9.

    Science.gov (United States)

    Sternberg, Samuel H; Doudna, Jennifer A

    2015-05-21

    Few discoveries transform a discipline overnight, but biologists today can manipulate cells in ways never possible before, thanks to a peculiar form of prokaryotic adaptive immunity mediated by clustered regularly interspaced short palindromic repeats (CRISPR). From elegant studies that deciphered how these immune systems function in bacteria, researchers quickly uncovered the technological potential of Cas9, an RNA-guided DNA cleaving enzyme, for genome engineering. Here we highlight the recent explosion in visionary applications of CRISPR-Cas9 that promises to usher in a new era of biological understanding and control.

  4. Applications of CRISPR-Cas systems in neuroscience.

    Science.gov (United States)

    Heidenreich, Matthias; Zhang, Feng

    2016-01-01

    Genome-editing tools, and in particular those based on CRISPR-Cas (clustered regularly interspaced short palindromic repeat (CRISPR)-CRISPR-associated protein) systems, are accelerating the pace of biological research and enabling targeted genetic interrogation in almost any organism and cell type. These tools have opened the door to the development of new model systems for studying the complexity of the nervous system, including animal models and stem cell-derived in vitro models. Precise and efficient gene editing using CRISPR-Cas systems has the potential to advance both basic and translational neuroscience research.

  5. Applications of CRISPR-Cas9 mediated genome engineering.

    Science.gov (United States)

    Yang, Xiao

    2015-01-01

    Targeted mutagenesis based on homologous recombination has been a powerful tool for understanding the mechanisms underlying development, normal physiology, and disease. A recent breakthrough in genome engineering technology based on the class of RNA-guided endonucleases, such as clustered regularly interspaced short palindromic repeats (CRISPR)-associated Cas9, is further revolutionizing biology and medical studies. The simplicity of the CRISPR-Cas9 system has enabled its widespread applications in generating germline animal models, somatic genome engineering, and functional genomic screening and in treating genetic and infectious diseases. This technology will likely be used in all fields of biomedicine, ranging from basic research to human gene therapy.

  6. [Sexuality education on the Internet : From Dr. Sommer to Dr. Google].

    Science.gov (United States)

    Döring, Nicola

    2017-07-24

    Female and male adolescents in Germany are increasingly using the Internet to find information about sexuality and sexual health. This review paper summarizes what we know about the status quo of online sexuality education in Germany.Based on a systematic literature review including 40 papers from international, peer-reviewed journals spanning 2010-2017, this paper first describes different aspects of the sexuality-related online search behavior of adolescents: its prevalence, predictors, topics and contexts. One main finding is the fact that adolescents use a computer or smartphone to type their sexuality-related questions into the search engine Google or the search engine of the video platform YouTube.Based on 54 online searches, this paper subsequently presents the kind of sexuality-related online content adolescents find if they ask "Dr. Google" for sexual advice; a collection of 1236 authentic sexuality-related questions of adolescents was used for this analysis. It turned out that online sexuality education offered by leading professional organizations like the BZgA ("Bundeszentrale für gesundheitliche Aufklärung") or pro familia was nearly invisible, while numerous other providers of online sex education consistently appeared in the top Google search results. Among them were the "Dr. Sommer" team of the youth magazine Bravo; online healthcare and advice portals; online forums; the online encyclopedia Wikipedia and, above all, sex education channels on YouTube. In this paper, the latter are presented in more detail for the first time.The third part of the paper addresses the quality of online sexual education over four main areas of quality evaluation. The presentation of the status quo ends with some recommendations both for future research and for sexuality education in practice.

  7. Educational Psychology as a Field and as a Career. Interview with César Coll

    Directory of Open Access Journals (Sweden)

    Marco Antonio Rigo Lemini

    2005-05-01

    Full Text Available This work offers an interview made by three Mexican scholars to Dr. César Coll from Barcelona University, who is one of the most well-known and influential educational psychologists in the Hispanic academic world. In this interview, Dr. Coll makes important contributions about themes such as: the Educational Psychology epistemological standing, the tendencies in the education of the professionals of this field, the new practices of the profession, some problems related to the accumulation and immeasurability of the psychology knowledge, and about the continuity of the academic and research groups.

  8. Psychology of psychology? A theoretization of psychological science through historical and socio-anthropological analysis of Psychology as institution

    OpenAIRE

    Martin Žužek-Kres

    2008-01-01

    The article presents a "new" history of psychology, which is also termed as "psychology of psychology". In some academic communities this unconventional history of psychology represents today an accepted approach to epistemological questions about psychological concepts and it enables an insight into social contextualization of Psychology as an institution. The conclusion presents a referential and institutional context where this psychology of psychology is realized.

  9. “No Cause for Action”: Revisiting the Ethics Case of Dr. John Leso

    Directory of Open Access Journals (Sweden)

    Roy J. Eidelson

    2015-07-01

    Full Text Available Recent revelations – from government documents and investigative reports – have brought renewed attention to the disturbing involvement of U.S. psychologists in the abuse and torture of “war on terror” detainees. In light of these revelations, this commentary examines the American Psychological Association's inexplicable decision to close – without any sanction – an ethics complaint filed against psychologist John Leso for his role in the abusive treatment of prisoners at the Guantanamo Bay detention facility. The essay reviews key components of the case: the relevant background and context, Dr. Leso’s documented actions at Guantanamo, the APA's ethical standards, the ethics complaint rules and procedures, the justifications invoked by the APA leadership to defend the no-sanction decision, and recommendations for what should be done now.

  10. Rheumatic fever in Ireland: the role of Dr Monica Lea Wilson (1889-1971).

    Science.gov (United States)

    Ward, O Conor

    2013-02-01

    In 1869 William Stokes pointed out that the severity of rheumatic fever in Dublin had declined over recent decades. Similar worldwide decline led to the closure of many internationally famous rheumatic fever centres. The discovery by Robert Collis that rheumatic fever was a sequel to haemolytic streptococcal infection and the subsequent discovery of penicillin accelerated the decline. St Gabriel's Hospital in Dublin opened in 1951 under the clinical direction of Dr Monica Lea Wilson. Contrary to contemporary medical opinion a regimen of very prolonged bed rest was enforced. From 1961 the family doctors became concerned at the adverse psychological effects of the unnecessarily prolonged hospital stay. Twenty-seven of the 56 inpatients were re-assessed. None of them showed any evidence of active rheumatic fever and their parents took them home. The hospital closed in 1968. Dr Lea Wilson distanced herself from mainstream medicine and she is best remembered for having presented an unrecognized Caravaggio painting to the Jesuit Order in recognition of their pastoral support at the time of the controversial assassination in 1920 of her husband Percival, an Inspector in the Royal Irish Constabulary.

  11. Remaining Sites Verification Package for 132-DR-1, 1608-DR Effluent Pumping Station, Waste Site Reclassification Form 2005-035

    Energy Technology Data Exchange (ETDEWEB)

    R. A. Carlson

    2005-09-22

    Radiological characterization, decommissioning and demolition of the 132-DR-1 site, 1608-DR Effluent Pumping Station was performed in 1987. The current site conditions achieve the remedial action objectives and the corresponding remedial action goals established in the Remaining Sites ROD. Residual concentrations support future land uses that can be represented by a rural-residential scenario and pose no threat to groundwater or the Columbia River based on RESRAD modeling.

  12. Volume celebrating the 60th birthday of Professor Dr. Franz Fie; Professor Dr. Franz Fiedler zum 60. Geburtstag

    Energy Technology Data Exchange (ETDEWEB)

    Beheng, K.D. [ed.

    1998-01-01

    This report was compiled in honor of Professor Dr. Franz Fiedler on the occasion of his 60th birthday on 7 January 1998. Its contributions deal with mesoscale modelling, the propagation of air pollutants, measurements of precipitation using radar, and prediction models. (orig./KW) [Deutsch] Dieser Bericht wurde aus Anlass des 60. Geburtstags von Herrn Prof. Dr. Franz Fiedler am 7. Januar 1998 zusammengestellt. Die Beitraege behandeln mesoskalige Modellierung, Ausbreitung von Luftschadstoffen, Niederschlagsmessung mit Radar und Vorhersagemodelle. (orig.)

  13. HLA DR4w4-binding motifs illustrate the biochemical basis of degeneracy and specificity in peptide-DR interactions.

    Science.gov (United States)

    Sette, A; Sidney, J; Oseroff, C; del Guercio, M F; Southwood, S; Arrhenius, T; Powell, M F; Colón, S M; Gaeta, F C; Grey, H M

    1993-09-15

    In the present study, we describe the definition of a DRB1*0401 (DR4w4)-specific motif. The strategy used entailed a three-step process: 1) screening a large set of unrelated peptide ligands to identify good MHC binders; 2) truncation analysis of several DR4w4 binding peptides of high affinity to identify the crucial core-binding regions; 3) the use of single amino acid substitutions of the DR4w4-binding peptide hemagglutinin (HA) 307-319 to elucidate the specific residues crucial for binding. The DR4w4 motif is characterized by the presence of a hydrophobic or aromatic (F, W, Y, L, I, V, M) anchor residue in position 1, and a second hydroxyl (S, T) or aliphatic (L, I, V, or M) anchor residue in position 6. Furthermore, positive charges (R, K) are not allowed in positions 4, 7, and 9, and negative charges (D, E) are not allowed in position 9. This motif was present in 92% of good (IC50 45 microM) binders, indicating that the presence of the motif is necessary, but not sufficient for good DR4w4 binding capacity. The results of the present study are discussed in relation to previous work defining binding motifs and rules for other DR alleles, illustrating how different DR alleles bind variations on a similar structural theme. Finally, using two different peptide ligands [tetanus toxoid 830-843 and HA 307-319] as model systems, it is demonstrated how the fine allelic specificity of the DR binders can be predictably modulated by introducing subtle changes in the primary amino acid sequence.

  14. Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease

    OpenAIRE

    Gratz, Scott J.; Cummings, Alexander M.; Nguyen, Jennifer N.; Hamm, Danielle C.; Donohue, Laura K.; Harrison, Melissa M; Wildonger, Jill; O’Connor-Giles, Kate M.

    2013-01-01

    We have adapted a bacterial CRISPR RNA/Cas9 system to precisely engineer the Drosophila genome and report that Cas9-mediated genomic modifications are efficiently transmitted through the germline. This RNA-guided Cas9 system can be rapidly programmed to generate targeted alleles for probing gene function in Drosophila.

  15. On the Integration of Computer Algebra Systems (CAS) by Canadian Mathematicians: Results of a National Survey

    Science.gov (United States)

    Buteau, Chantal; Jarvis, Daniel H.; Lavicza, Zsolt

    2014-01-01

    In this article, we outline the findings of a Canadian survey study (N = 302) that focused on the extent of computer algebra systems (CAS)-based technology use in postsecondary mathematics instruction. Results suggest that a considerable number of Canadian mathematicians use CAS in research and teaching. CAS use in research was found to be the…

  16. The role of Cas8 in type I CRISPR interference.

    Science.gov (United States)

    Cass, Simon D B; Haas, Karina A; Stoll, Britta; Alkhnbashi, Omer S; Sharma, Kundan; Urlaub, Henning; Backofen, Rolf; Marchfelder, Anita; Bolt, Edward L

    2015-05-05

    CRISPR (clustered regularly interspaced short palindromic repeat) systems provide bacteria and archaea with adaptive immunity to repel invasive genetic elements. Type I systems use 'cascade' [CRISPR-associated (Cas) complex for antiviral defence] ribonucleoprotein complexes to target invader DNA, by base pairing CRISPR RNA (crRNA) to protospacers. Cascade identifies PAMs (protospacer adjacent motifs) on invader DNA, triggering R-loop formation and subsequent DNA degradation by Cas3. Cas8 is a candidate PAM recognition factor in some cascades. We analysed Cas8 homologues from type IB CRISPR systems in archaea Haloferax volcanii (Hvo) and Methanothermobacter thermautotrophicus (Mth). Cas8 was essential for CRISPR interference in Hvo and purified Mth Cas8 protein responded to PAM sequence when binding to nucleic acids. Cas8 interacted physically with Cas5-Cas7-crRNA complex, stimulating binding to PAM containing substrates. Mutation of conserved Cas8 amino acid residues abolished interference in vivo and altered catalytic activity of Cas8 protein in vitro. This is experimental evidence that Cas8 is important for targeting Cascade to invader DNA. © 2015 Authors.

  17. Introduction to Psychology.

    Science.gov (United States)

    Edwards, Lesley

    Designed for community students interested in learning about psychology as a field of study, this module offers group and individual activities to involve the beginning student in research, experimentation and discussion. Unit 1, "What Is Psychology?," includes the use of animals in psychology, ethics, the history of psychology, an…

  18. Introduction to Psychology.

    Science.gov (United States)

    Edwards, Lesley

    Designed for community students interested in learning about psychology as a field of study, this module offers group and individual activities to involve the beginning student in research, experimentation and discussion. Unit 1, "What Is Psychology?," includes the use of animals in psychology, ethics, the history of psychology, an…

  19. Mainstreaming Culture in Psychology

    Science.gov (United States)

    Cheung, Fanny M.

    2012-01-01

    Despite the "awakening" to the importance of culture in psychology in America, international psychology has remained on the sidelines of psychological science. The author recounts her personal and professional experience in tandem with the stages of development in international/cross-cultural psychology. Based on her research in cross-cultural…

  20. A Comparison of the SB5 and the CAS in Educational Psychology Practice

    Science.gov (United States)

    Berman, Jeanette; Price, Ian

    2013-01-01

    Two tests of intellectual ability were compared in terms of quantitative measures and professional utility in the context of 41 students who were referred for psychoeducational investigation of their learning. Full-scale, Composite, and Factor scores from The Stanford Binet Intelligence Scales--Fifth Edition (SB5) and the Das Naglieri Cognitive…

  1. Technological Discourse on CAS-Based Operative Knowledge

    Science.gov (United States)

    Mann, Giora; Dana-Picard, Thierry; Zehavi, Nurit

    2007-01-01

    This article begins with a comparison of two groups of teachers, working on the same tasks in Analytic Geometry. One group has only basic experience in CAS-assisted problem solving, and the other group has extensive experience. The comparison is discussed in terms of the interplay between reflection, operative knowledge and execution. The findings…

  2. Is there a propeller neutron star in γ Cas?

    Science.gov (United States)

    Smith, M. A.; Lopes de Oliveira, R.; Motch, C.

    2017-08-01

    γ Cas is the prototype of a small population of B0-B1.5 III-V classical Be (cBe) stars that emit anomalous and hard X-rays with a unique array of properties. γ Cas is known to host, like other cBe stars, a decretion disc and also a low-mass companion. Recently, Postnov et al. have posited that this companion is a magnetized rapidly spinning neutron star that deflects direct gravitational accretion from a stellar/disc wind via the 'propeller mechanism'. These authors state that the key X-ray observations are 'remarkably well produced' in this scenario. We re-examine this mechanism in detail and conclude that there are a number of fatal objections in its application to the γ Cas case. Among other considerations these issues include the prediction under the propeller scenario of a much smaller population of γ Cas stars than is observed and the lack of allowance for observed correlations of X-ray and UV and/or optical properties over a variety of time-scales.

  3. A Decade of Development of the Bairen Program at CAS

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    @@ Launched in 1994, the Bairen Program is a major initiative of CAS to speed up its cultivation of disciplinary pacesetters and bridge the generation gap of scientific personnel. As a pioneering move of its kind in this country, the Program is noted for its ambitious goals, high standards and strong support.

  4. Evolution and classification of the CRISPR-Cas systems

    NARCIS (Netherlands)

    Makarova, K.S.; Brouns, S.J.J.; Oost, van der J.

    2011-01-01

    The CRISPR-Cas (clustered regularly interspaced short palindromic repeats-CRISPR-associated proteins) modules are adaptive immunity systems that are present in many archaea and bacteria. These defence systems are encoded by operons that have an extraordinarily diverse architecture and a high rate of

  5. Accretion Events in Binary Systems: AZ Cas and VV Cep

    CERN Document Server

    Gałan, C; Tomov, T; Wiȩcek, M; Majcher, A; Wychudzki, P; Świerczyński, E; Kolev, D; Brożek, T; Maciejewski, G; Zoła, S; Kurpińska-Winiarska, M; Winiarski, M; Ogłoza, W; Drożdż, M; Krzesiński, J

    2011-01-01

    The sudden lengthening of orbital period of VV Cep eclipsing binary by about 1% was observed in the last epoch. The mass transfer and/or mass loss are most possible explanations of this event. The photometric behaviour of AZ Cas, the cousin of VV Cep, suggests that the accretion can occur and could be important in this system, too.

  6. Recent Achievements Scored by CAS Scientists in Life Sciences

    Institute of Scientific and Technical Information of China (English)

    2004-01-01

    @@ Rice Genome Project for Oryza sativa L. Ssp. Indica After 17-month hard work, the scientists of the CAS Beijing Genomics Institute accomplished the draft sequence of the rice genome for Oryza sativa L. Ssp. Indica in October, 2001, and the data were unconditionally shared by the whole world. About 270,000 people had visited Chinese rice genome website by May 2003.

  7. CRISPR/Cas9 advances engineering of microbial cell factories.

    Science.gov (United States)

    Jakočiūnas, Tadas; Jensen, Michael K; Keasling, Jay D

    2016-03-01

    One of the key drivers for successful metabolic engineering in microbes is the efficacy by which genomes can be edited. As such there are many methods to choose from when aiming to modify genomes, especially those of model organisms like yeast and bacteria. In recent years, clustered regularly interspaced palindromic repeats (CRISPR) and its associated proteins (Cas) have become the method of choice for precision genome engineering in many organisms due to their orthogonality, versatility and efficacy. Here we review the strategies adopted for implementation of RNA-guided CRISPR/Cas9 genome editing with special emphasis on their application for metabolic engineering of yeast and bacteria. Also, examples of how nuclease-deficient Cas9 has been applied for RNA-guided transcriptional regulation of target genes will be reviewed, as well as tools available for computer-aided design of guide-RNAs will be highlighted. Finally, this review will provide a perspective on the immediate challenges and opportunities foreseen by the use of CRISPR/Cas9 genome engineering and regulation in the context of metabolic engineering.

  8. Novel progress in clean energy partnership between CAS and BP

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    @@ On behalf of their respective organizations,CAS Vice President LI Jinghai and BP Group Chief Executive lain Conn recently put their names on an agreement for the partner selection and technology roadmap principle for the Clean Energy Commercialization Center (CECC),a joint venture between the two sides.

  9. Evolution and classification of the CRISPR-Cas systems

    NARCIS (Netherlands)

    Makarova, K.S.; Brouns, S.J.J.; Oost, van der J.

    2011-01-01

    The CRISPR-Cas (clustered regularly interspaced short palindromic repeats-CRISPR-associated proteins) modules are adaptive immunity systems that are present in many archaea and bacteria. These defence systems are encoded by operons that have an extraordinarily diverse architecture and a high rate of

  10. Building the Class 2 CRISPR-Cas Arsenal.

    Science.gov (United States)

    Lewis, Kevin M; Ke, Ailong

    2017-02-02

    Adaptation of CRISPR-Cas9 for genome-editing applications has revolutionized biomedical research. New single-component effector CRISPR systems are emerging from the bioinformatics pipeline. How can we best harness their power? Three new studies will no doubt facilitate this transition by generating the C2c1 and C2c2 structure snapshots in different functional states.

  11. Preparing Students to Take SOA/CAS Exam FM/2

    Science.gov (United States)

    Marchand, Richard J.

    2014-01-01

    This paper provides suggestions for preparing students to take the actuarial examination on financial mathematics, SOA/CAS Exam FM/2. It is based on current practices employed at Slippery Rock University, a small public liberal arts university. Detailed descriptions of our Theory of Interest course and subsequent Exam FM/2 prep course are provided…

  12. LES KYSTES AMYGDALOIDES : A PROPOS DE 31 CAS SECOND ...

    African Journals Online (AJOL)

    Conclusion: In the presence of a cystic lesion of the lateral neck, even though the lesion represents ... Un trismus a été noté dans un cas. .... possibilité de métastases de cancer amygdalien homolaté- ... Otolaryngeal Head Neck Surg 1993;.

  13. Cas9-Guide RNA Directed Genome Editing in Soybean.

    Science.gov (United States)

    Li, Zhongsen; Liu, Zhan-Bin; Xing, Aiqiu; Moon, Bryan P; Koellhoffer, Jessica P; Huang, Lingxia; Ward, R Timothy; Clifton, Elizabeth; Falco, S Carl; Cigan, A Mark

    2015-10-01

    Recently discovered bacteria and archaea adaptive immune system consisting of clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) endonuclease has been explored in targeted genome editing in different species. Streptococcus pyogenes Cas9-guide RNA (gRNA) was successfully applied to generate targeted mutagenesis, gene integration, and gene editing in soybean (Glycine max). Two genomic sites, DD20 and DD43 on chromosome 4, were mutagenized with frequencies of 59% and 76%, respectively. Sequencing randomly selected transgenic events confirmed that the genome modifications were specific to the Cas9-gRNA cleavage sites and consisted of small deletions or insertions. Targeted gene integrations through homology-directed recombination were detected by border-specific polymerase chain reaction analysis for both sites at callus stage, and one DD43 homology-directed recombination event was transmitted to T1 generation. T1 progenies of the integration event segregated according to Mendelian laws and clean homozygous T1 plants with the donor gene precisely inserted at the DD43 target site were obtained. The Cas9-gRNA system was also successfully applied to make a directed P178S mutation of acetolactate synthase1 gene through in planta gene editing.

  14. Cas9-based genome editing in Arabidopsis and tobacco.

    Science.gov (United States)

    Li, Jian-Feng; Zhang, Dandan; Sheen, Jen

    2014-01-01

    Targeted modification of plant genome is key to elucidating and manipulating gene functions in plant research and biotechnology. The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) technology is emerging as a powerful genome-editing method in diverse plants that traditionally lacked facile and versatile tools for targeted genetic engineering. This technology utilizes easily reprogrammable guide RNAs (sgRNAs) to direct Streptococcus pyogenes Cas9 endonuclease to generate DNA double-stranded breaks in targeted genome sequences, which facilitates efficient mutagenesis by error-prone nonhomologous end-joining (NHEJ) or sequence replacement by homology-directed repair (HDR). In this chapter, we describe the procedure to design and evaluate dual sgRNAs for plant codon-optimized Cas9-mediated genome editing using mesophyll protoplasts as model cell systems in Arabidopsis thaliana and Nicotiana benthamiana. We also discuss future directions in sgRNA/Cas9 applications for generating targeted genome modifications and gene regulations in plants.

  15. World's largest robotic telescope goes into operation at CAS

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    @@ Lijiang 2.4, one of the world largest fully robotic astronomical telescopes, was put into operation on 12 May at the CAS Yunnan Observatory, the highest of four professional astronomical observatories in China and the only one in the south part of the country.

  16. CAS academician honored at 4th world urban forum

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    @@ Prof. Anthony Yeh, a CAS Member from the University of Hong Kong (HKU), is selected the winner of the 2008 UN-HABITAT Lecture Award--the highest international acclaims in the town planning field. The award is to recognize outstanding and sustained contributions to research, thinking and practice in human settlements development and planning. Prof.

  17. Medical marijuana: CAS releases report, government cuts research funding.

    Science.gov (United States)

    Betteridge, Glenn

    2006-12-01

    In June 2006, the Canadian AIDS Society (CAS) released a comprehensive report with recommendations to overcome barriers to the use of cannabis for medical purposes faced by people living with HIV/AIDS in Canada. On 25 September 2006, as part of package of spending cuts, the federal government announced plans to eliminate its marijuana medical research program.

  18. Young scientific stars from CAS receive national award

    Institute of Scientific and Technical Information of China (English)

    2012-01-01

    Fifteen brilliant young researchers from CAS institutes received the 12th National Award for Youth in Science and Technology at a special ceremony held in Beijing on December 15, 2011. They include WANG Shu with the Institute of Chemistry, TIAN Ye and YU Le'an with the Academy of Mathematics and Systems Science, LI Jia with the Shanghai Institute of Materia Medica,

  19. Case report basedow: à propos d'un cas

    African Journals Online (AJOL)

    abp

    12 janv. 2017 ... article est de présenter un cas de maladie de Basedow chez l'enfant dont le facteur déclenchant inhabituel serait la ... Il est né au terme d'une grossesse suivie .... JC, Alonso M. Graves' disease in children and adolescents:.

  20. An updated evolutionary classification of CRISPR-Cas systems

    NARCIS (Netherlands)

    Makarova, K.S.; Wolf, Y.I.; Alkhnbashi, O.S.; Brouns, S.J.J.; Oost, Van Der John

    2015-01-01

    The evolution of CRISPR-cas loci, which encode adaptive immune systems in archaea and bacteria, involves rapid changes, in particular numerous rearrangements of the locus architecture and horizontal transfer of complete loci or individual modules. These dynamics complicate straightforward phyloge

  1. Maximizing mutagenesis with solubilized CRISPR-Cas9 ribonucleoprotein complexes.

    Science.gov (United States)

    Burger, Alexa; Lindsay, Helen; Felker, Anastasia; Hess, Christopher; Anders, Carolin; Chiavacci, Elena; Zaugg, Jonas; Weber, Lukas M; Catena, Raul; Jinek, Martin; Robinson, Mark D; Mosimann, Christian

    2016-06-01

    CRISPR-Cas9 enables efficient sequence-specific mutagenesis for creating somatic or germline mutants of model organisms. Key constraints in vivo remain the expression and delivery of active Cas9-sgRNA ribonucleoprotein complexes (RNPs) with minimal toxicity, variable mutagenesis efficiencies depending on targeting sequence, and high mutation mosaicism. Here, we apply in vitro assembled, fluorescent Cas9-sgRNA RNPs in solubilizing salt solution to achieve maximal mutagenesis efficiency in zebrafish embryos. MiSeq-based sequence analysis of targeted loci in individual embryos using CrispRVariants, a customized software tool for mutagenesis quantification and visualization, reveals efficient bi-allelic mutagenesis that reaches saturation at several tested gene loci. Such virtually complete mutagenesis exposes loss-of-function phenotypes for candidate genes in somatic mutant embryos for subsequent generation of stable germline mutants. We further show that targeting of non-coding elements in gene regulatory regions using saturating mutagenesis uncovers functional control elements in transgenic reporters and endogenous genes in injected embryos. Our results establish that optimally solubilized, in vitro assembled fluorescent Cas9-sgRNA RNPs provide a reproducible reagent for direct and scalable loss-of-function studies and applications beyond zebrafish experiments that require maximal DNA cutting efficiency in vivo.

  2. CRISPR-Cas9: from Genome Editing to Cancer Research.

    Science.gov (United States)

    Chen, Si; Sun, Heng; Miao, Kai; Deng, Chu-Xia

    2016-01-01

    Cancer development is a multistep process triggered by innate and acquired mutations, which cause the functional abnormality and determine the initiation and progression of tumorigenesis. Gene editing is a widely used engineering tool for generating mutations that enhance tumorigenesis. The recent developed clustered regularly interspaced short palindromic repeats-CRISPR-associated 9 (CRISPR-Cas9) system renews the genome editing approach into a more convenient and efficient way. By rapidly introducing genetic modifications in cell lines, organs and animals, CRISPR-Cas9 system extends the gene editing into whole genome screening, both in loss-of-function and gain-of-function manners. Meanwhile, the system accelerates the establishment of animal cancer models, promoting in vivo studies for cancer research. Furthermore, CRISPR-Cas9 system is modified into diverse innovative tools for observing the dynamic bioprocesses in cancer studies, such as image tracing for targeted DNA, regulation of transcription activation or repression. Here, we view recent technical advances in the application of CRISPR-Cas9 system in cancer genetics, large-scale cancer driver gene hunting, animal cancer modeling and functional studies.

  3. Genome Editing with CRISPR-Cas9: Can It Get Any Better?

    Science.gov (United States)

    Haeussler, Maximilian; Concordet, Jean-Paul

    2016-05-20

    The CRISPR-Cas revolution is taking place in virtually all fields of life sciences. Harnessing DNA cleavage with the CRISPR-Cas9 system of Streptococcus pyogenes has proven to be extraordinarily simple and efficient, relying only on the design of a synthetic single guide RNA (sgRNA) and its co-expression with Cas9. Here, we review the progress in the design of sgRNA from the original dual RNA guide for S. pyogenes and Staphylococcus aureus Cas9 (SpCas9 and SaCas9). New assays for genome-wide identification of off-targets have provided important insights into the issue of cleavage specificity in vivo. At the same time, the on-target activity of thousands of guides has been determined. These data have led to numerous online tools that facilitate the selection of guide RNAs in target sequences. It appears that for most basic research applications, cleavage activity can be maximized and off-targets minimized by carefully choosing guide RNAs based on computational predictions. Moreover, recent studies of Cas proteins have further improved the flexibility and precision of the CRISPR-Cas toolkit for genome editing. Inspired by the crystal structure of the complex of sgRNA-SpCas9 bound to target DNA, several variants of SpCas9 have recently been engineered, either with novel protospacer adjacent motifs (PAMs) or with drastically reduced off-targets. Novel Cas9 and Cas9-like proteins called Cpf1 have also been characterized from other bacteria and will benefit from the insights obtained from SpCas9. Genome editing with CRISPR-Cas9 may also progress with better understanding and control of cellular DNA repair pathways activated after Cas9-induced DNA cleavage.

  4. Overall properties of the Gaia DR1 reference frame

    Science.gov (United States)

    Liu, N.; Zhu, Z.; Liu, J.-C.; Ding, C.-Y.

    2017-03-01

    Aims: The first Gaia data release (Gaia DR1) provides 2191 ICRF2 sources with their positions in the auxiliary quasar solution and five astrometric parameters - positions, parallaxes, and proper motions - for stars in common between the Tycho-2 catalogue and Gaia in the joint Tycho-Gaia astrometric solution (TGAS). We aim to analyze the overall properties of Gaia DR1 reference frame. Methods: We compare quasar positions of the auxiliary quasar solution with ICRF2 sources using different samples and evaluate the influence on the Gaia DR1 reference frame owing to the Galactic aberration effect over the J2000.0-J2015.0 period. Then we estimate the global rotation between TGAS with Tycho-2 proper motion systems to investigate the property of the Gaia DR1 reference frame. Finally, the Galactic kinematics analysis using the K-M giant proper motions is performed to understand the property of Gaia DR1 reference frame. Results: The positional comparison between the auxiliary quasar solution and ICRF2 shows negligible orientation and validates the declination bias of -0.1mas in Gaia quasar positions with respect to ICRF2. Galactic aberration effect is thought to cause an offset 0.01mas of the Z axis direction of Gaia DR1 reference frame. The global rotation between TGAS and Tycho-2 proper motion systems, obtained by different samples, shows a much smaller value than the claimed value 0.24mas yr-1. For the Galactic kinematics analysis of the TGAS K-M giants, we find possible non-zero Galactic rotation components beyond the classical Oort constants: the rigid part ωYG = -0.38±0.15mas yr-1 and the differential part ω^primeYG = -0.29±0.19mas yr-1 around the YG axis of Galactic coordinates, which indicates possible residual rotation in Gaia DR1 reference frame or problems in the current Galactic kinematical model. Conclusions: The Gaia DR1 reference frame is well aligned to ICRF2, and the possible influence of the Galactic aberration effect should be taken into consideration

  5. Direct Cytosolic Delivery of CRISPR/Cas9-Ribonucleoprotein for Efficient Gene Editing.

    Science.gov (United States)

    Mout, Rubul; Ray, Moumita; Yesilbag Tonga, Gulen; Lee, Yi-Wei; Tay, Tristan; Sasaki, Kanae; Rotello, Vincent M

    2017-03-28

    Genome editing through the delivery of CRISPR/Cas9-ribonucleoprotein (Cas9-RNP) reduces unwanted gene targeting and avoids integrational mutagenesis that can occur through gene delivery strategies. Direct and efficient delivery of Cas9-RNP into the cytosol followed by translocation to the nucleus remains a challenge. Here, we report a remarkably highly efficient (∼90%) direct cytoplasmic/nuclear delivery of Cas9 protein complexed with a guide RNA (sgRNA) through the coengineering of Cas9 protein and carrier nanoparticles. This construct provides effective (∼30%) gene editing efficiency and opens up opportunities in studying genome dynamics.

  6. HLA-DR-positive cells in large plaque (atrophic) parapsoriasis.

    Science.gov (United States)

    McMillan, E M; Wasik, R; Everett, M A

    1981-10-01

    The development of a monoclonal antibody directed against HLA DR (Ia-like) antigens of B cells and monocytes but not against normal peripheral human T cells suggested that this antibody might be used as a marker of B cells and monocytes in tissue sections. The T cell nature of large plaque (atrophic) parapsoriasis has recently been demonstrated by the immunoperoxidase technic. Immunoperoxidase examination of serial sections of tissues from two cases of large plaque parapsoriasis with one T cell antiserum, two monoclonal T cell antibodies, and one monoclonal reagent directed against HLA DR indicated that T cells in the cutaneous infiltrates were also HLA DR-positive. Evidence is accumulating that HLA DR positivity may be expressed by activated T cells. The findings here therefore suggest that many of the T lymphoid cells in two cases of large plaque (atrophic) parapsoriasis examined were activated in nature, and that HLA DR may not be a specific marker for B cells and monocytes in certain pathologic conditions. Caution should therefore presently be exercised in attempting to use this marker for the specific identification of B cells and monocytes in pathologic specimens, without simultaneous testing for T cell markers.

  7. DR WHO: a workshop for house officer preparation.

    Science.gov (United States)

    Cave, Judith; Wallace, Deirdre; Baillie, Glenda; Klingenberg, Michael; Phillips, Catherine; Oliver, Harriet; Rowles, Katherine; Dunkley, Lisa; Sturrock, Alison; Dacre, Jane

    2007-01-01

    Newly qualified doctors should be competent in advanced life support (ALS) and critical care. The Resuscitation Council has published a course about ALS for undergraduate medical students (the intermediate life support (ILS) course). However, there is no undergraduate-level course on assessing and treating critically ill patients, despite the fact that postgraduate courses on this topic are extremely popular. We have developed a new course called Direct Response Workshop for House Officer Preparation (DR WHO), which teaches both ALS and critical care at an undergraduate level. We taught the Resuscitation Council ILS course to our 2003-4 cohort of final year medical students (n = 350), and the new course (DR WHO) to our 2004-5 cohort (n = 338). Students filled in feedback forms immediately after the courses, and a subset repeated the feedback forms 4 months after they had started work as house officers. Course evaluation: Student and house officer feedback was positive. The DR WHO cohort was more confident in caring for critically ill patients (18/26 (69%) were confident after ILS, and 40/45 (89%) were confident after DR WHO (chi2 = 4.3; df = 1; p = 0.06)). Both cohorts were competent in ALS, each with a mean score of 18.6/20 in a finals level practical examination on this topic. The DR WHO course is popular with the students and practical to run. The course needs to be re-evaluated to determine the long-term competency of graduates.

  8. Mobile CRISPR/Cas-mediated bacteriophage resistance in Lactococcus lactis.

    Directory of Open Access Journals (Sweden)

    Anne M Millen

    Full Text Available Lactococcus lactis is a biotechnological workhorse for food fermentations and potentially therapeutic products and is therefore widely consumed by humans. It is predominantly used as a starter microbe for fermented dairy products, and specialized strains have adapted from a plant environment through reductive evolution and horizontal gene transfer as evidenced by the association of adventitious traits with mobile elements. Specifically, L. lactis has armed itself with a myriad of plasmid-encoded bacteriophage defensive systems to protect against viral predation. This known arsenal had not included CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR-associated proteins, which forms a remarkable microbial immunity system against invading DNA. Although CRISPR/Cas systems are common in the genomes of closely related lactic acid bacteria (LAB, none was identified within the eight published lactococcal genomes. Furthermore, a PCR-based search of the common LAB CRISPR/Cas systems (Types I and II in 383 industrial L. lactis strains proved unsuccessful. Here we describe a novel, Type III, self-transmissible, plasmid-encoded, phage-interfering CRISPR/Cas discovered in L. lactis. The native CRISPR spacers confer resistance based on sequence identity to corresponding lactococcal phage. The interference is directed at phages problematic to the dairy industry, indicative of a responsive system. Moreover, targeting could be modified by engineering the spacer content. The 62.8-kb plasmid was shown to be conjugally transferrable to various strains. Its mobility should facilitate dissemination within microbial communities and provide a readily applicable system to naturally introduce CRISPR/Cas to industrially relevant strains for enhanced phage resistance and prevention against acquisition of undesirable genes.

  9. CRISPR/Cas9-mediated targeted mutagenesis in Nicotiana tabacum.

    Science.gov (United States)

    Gao, Junping; Wang, Genhong; Ma, Sanyuan; Xie, Xiaodong; Wu, Xiangwei; Zhang, Xingtan; Wu, Yuqian; Zhao, Ping; Xia, Qingyou

    2015-01-01

    Genome editing is one of the most powerful tools for revealing gene function and improving crop plants. Recently, RNA-guided genome editing using the type II clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein (Cas) system has been used as a powerful and efficient tool for genome editing in various organisms. Here, we report genome editing in tobacco (Nicotiana tabacum) mediated by the CRISPR/Cas9 system. Two genes, NtPDS and NtPDR6, were used for targeted mutagenesis. First, we examined the transient genome editing activity of this system in tobacco protoplasts, insertion and deletion (indel) mutations were observed with frequencies of 16.2-20.3% after transfecting guide RNA (gRNA) and the nuclease Cas9 in tobacco protoplasts. The two genes were also mutated using multiplexing gRNA at a time. Additionally, targeted deletions and inversions of a 1.8-kb fragment between two target sites in the NtPDS locus were demonstrated, while indel mutations were also detected at both the sites. Second, we obtained transgenic tobacco plants with NtPDS and NtPDR6 mutations induced by Cas9/gRNA. The mutation percentage was 81.8% for NtPDS gRNA4 and 87.5% for NtPDR6 gRNA2. Obvious phenotypes were observed, etiolated leaves for the psd mutant and more branches for the pdr6 mutant, indicating that highly efficient biallelic mutations occurred in both transgenic lines. No significant off-target mutations were obtained. Our results show that the CRISPR/Cas9 system is a useful tool for targeted mutagenesis of the tobacco genome.

  10. Guidelines for Mental Health Interventions during Disasters at the “Dr. Gustavo Aldereguía

    Directory of Open Access Journals (Sweden)

    Teresa Rodríguez Rodríguez

    2010-02-01

    Full Text Available These guidelines for mental health intervention within a hospital in cases of catastrophic situations have been prepared not only under the national standards but under the Pan-American Health Organization ones taking into account the specialists and experts’ experience on the topic and it has had the approval of the Scientific Council of the University General Hospital “Dr. Gustavo Aldereguía Lima” from Cienfuegos. The guidelines describe the group of procedures to develop in the different cycles of disaster reduction (planning, quip and recovery mainly those related to patients, family members and hospital staff. This written record gathers the main psychological symptoms and diseases that result from these difficult situations and the main psychological techniques for such situations.

  11. Fusion of SpCas9 to E.coli Rec A protein enhances CRISPR-Cas9 mediated gene knockout in mammalian cells

    DEFF Research Database (Denmark)

    Lin, Lin; Petersen, Trine Skov; Jensen, Kristopher Torp

    2017-01-01

    Mammalian cells repair double-strand DNA breaks (DSB) by a range of different pathways following DSB induction by the engineered clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein Cas9. While CRISPR-Cas9 thus enables predesigned modifications of the genome......, applications of CRISPR-Cas9-mediated genome-editing are frequently hampered by the unpredictable and varying pathways for DSB repair in mammalian cells. Here we present a strategy of fusing Cas9 to recombinant proteins for fine-tuning of the DSB repair preferences in mammalian cells. By fusing Streptococcus...

  12. Statistical Study of 2XMMi-DR3/SDSS-DR8 Cross-correlation Sample

    CERN Document Server

    Yan-Xia, Zhang; Yong-Heng, Zhao; Xue-Bing, Wu; 10.1088/0004-6256/145/2/42

    2013-01-01

    Cross-correlating the XMM-Newton 2XMMi-DR3 catalog with the Sloan Digital Sky Survey (SDSS) Data Release 8, we obtain one of the largest X-ray/optical catalogs and explore the distribution of various classes of X-ray emitters in the multidimensional photometric parameter space. Quasars and galaxies occupy different zones while stars scatter in them. However, X-ray active stars have a certain distributing rule according to spectral types. The earlier the type of stars, the stronger X-ray emitting. X-ray active stars have a similar distribution to most of stars in the g-r versus r-i diagram. Based on the identified samples with SDSS spectral classification, a random forest algorithm for automatic classification is performed. The result shows that the classification accuracy of quasars and galaxies adds up to more than 93.0% while that of X-ray emitting stars only amounts to 45.3%. In other words, it is easy to separate quasars and galaxies, but it is difficult to discriminate X-ray active stars from quasars and...

  13. CRISPR/Cas9技术研究进展%Progress of CRISPR/Cas9 system

    Institute of Scientific and Technical Information of China (English)

    李和刚; 杨峰; 张宝珣; 李培培; 王建华; 包汉勋; 苗刚; 江科; 柳楠

    2015-01-01

    CRISPR/Cas9是近几年发展起来的基因组编辑技术,它为基因组工程领域带来了新的革命.Cas9可以在20nt左右的向导RNA介导下切割特定的基因组位点,而且几乎可以在任何物种的任何位置发挥作用.而且Cas9只要稍加改造,就可以应用于基因表达调控、表观基因组修饰及活体成像领域.本文综述了CRISPR/Cas9系统的发展历程、作用机理及其所面临的挑战,重点论述了在医学、生物技术及畜牧领域的应用现状及前景.

  14. Structural plasticity and in vivo activity of Cas1 from the type I-F CRISPR-Cas system

    NARCIS (Netherlands)

    Wilkinson, Max E.; Nakatani, Yoshio; Staals, Raymond H.J.; Kieper, Sebastian N.; Opel-Reading, Helen K.; McKenzie, Rebecca E.; Fineran, Peter C.; Krause, Kurt L.

    2016-01-01

    CRISPR-Cas systems are adaptive immune systems in prokaryotes that provide protection against viruses and other foreign DNA. In the adaptation stage, foreign DNA is integrated into CRISPR (clustered regularly interspaced short palindromic repeat) arrays as new spacers. These spacers are used in t

  15. HMMCAS: a web tool for the identification and domain annotations of Cas proteins.

    Science.gov (United States)

    Chai, Guoshi; Yu, Min; Jiang, Lixu; Duan, Yaocong; Huang, Jian

    2017-02-07

    The CRISPR-Cas (clustered regularly interspaced short palindromic repeats-CRISPR-associated proteins) adaptive immune systems are discovered in many bacteria and most archaea. These systems are encoded by cas (CRISPR-associated) operons that have an extremely diverse architecture. The most crucial step in the depiction of cas operons composition is the identification of cas genes or Cas proteins. With the continuous increase of the newly sequenced archaeal and bacterial genomes, the recognition of new Cas proteins is becoming possible, which not only provides candidates for novel genome editing tools but also helps to understand the prokaryotic immune system better. Here we describe HMMCAS, a web service for the detection of CRISPR-associated structural and functional domains in protein sequences. HMMCAS uses hmmscan similarity search algorithm in HMMER3.1 to provide a fast, interactive service based on a comprehensive collection of hidden Markov models of Cas protein family. It can accurately identify the Cas proteins including those fusion proteins, for example the Cas1-Cas4 fusion protein in Candidatus Chloracidobacterium thermophilum B (Cab. thermophilum B). HMMCAS can also find putative cas operon and determine which type it belongs to. HMMCAS is freely available at http://i.uestc.edu.cn/hmmcas.

  16. Historizing epistemology in psychology.

    Science.gov (United States)

    Jovanović, Gordana

    2010-12-01

    The conflict between the psychometric methodological framework and the particularities of human experiences reported in psychotherapeutic context led Michael Schwarz to raise the question whether psychology is based on a methodological error. I take this conflict as a heuristic tool for the reconstruction of the early history of psychology, which bears witness to similar epistemological conflicts, though the dominant historiography of psychology has largely forgotten alternative conceptions and their valuable insights into complexities of psychic phenomena. In order to work against the historical amnesia in psychology I suggest to look at cultural-historical contexts which decisively shaped epistemological choices in psychology. Instead of keeping epistemology and history of psychology separate, which nurtures individualism and naturalism in psychology, I argue for historizing epistemology and for historical psychology. From such a historically reflected perspective psychology in contemporary world can be approached more critically.

  17. Psychological Theories of Acculturation

    DEFF Research Database (Denmark)

    Ozer, Simon

    2017-01-01

    The proliferation of cultural transition and intercultural contact has highlighted the importance of psychological theories of acculturation. Acculturation, understood as contact between diverse cultural streams, has become prevalent worldwide due to technological, economical, and educational...... advancements, together with greater mobility. Acculturation psychology aims to comprehend the dynamic psychological processes and outcomes emanating from intercultural contact. Acculturation psychology has been a growing field of research within cross-cultural psychology. Today, psychological theories...... of acculturation also include cognate disciplines such as cultural psychology, social psychology, sociology, and anthropology.The expansion of psychological theories of acculturation has led to advancements in the field of research as well as the bifurcation of epistemological and methodological approaches...

  18. Professor Bakytzhan Abdiraiym Rector of the L. Gumilov Eurasian National University, Astana, Kazakhstan accompanied by Prof. Kairat Kuterbekov, Dr Bekzat Prmantayeva, Dr Kuralay Maksut with the Director-General, Dr Tadeusz Kurtyka, Adviser for Non-Member States, Mrs Julia Andreeva, Department of Information Technologies and Dr Nikolai Zimine, ATLAS Collaboration, Joint Institute for Nuclear Research, Dubna

    CERN Multimedia

    Maximilien Brice

    2011-01-01

    Professor Bakytzhan Abdiraiym Rector of the L. Gumilov Eurasian National University, Astana, Kazakhstan accompanied by Prof. Kairat Kuterbekov, Dr Bekzat Prmantayeva, Dr Kuralay Maksut with the Director-General, Dr Tadeusz Kurtyka, Adviser for Non-Member States, Mrs Julia Andreeva, Department of Information Technologies and Dr Nikolai Zimine, ATLAS Collaboration, Joint Institute for Nuclear Research, Dubna

  19. CRISPR/Cas9 for genome editing: progress, implications and challenges.

    Science.gov (United States)

    Zhang, Feng; Wen, Yan; Guo, Xiong

    2014-09-15

    Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) protein 9 system provides a robust and multiplexable genome editing tool, enabling researchers to precisely manipulate specific genomic elements, and facilitating the elucidation of target gene function in biology and diseases. CRISPR/Cas9 comprises of a nonspecific Cas9 nuclease and a set of programmable sequence-specific CRISPR RNA (crRNA), which can guide Cas9 to cleave DNA and generate double-strand breaks at target sites. Subsequent cellular DNA repair process leads to desired insertions, deletions or substitutions at target sites. The specificity of CRISPR/Cas9-mediated DNA cleavage requires target sequences matching crRNA and a protospacer adjacent motif locating at downstream of target sequences. Here, we review the molecular mechanism, applications and challenges of CRISPR/Cas9-mediated genome editing and clinical therapeutic potential of CRISPR/Cas9 in future.

  20. A simplified and efficient germline-specific CRISPR/Cas9 system for Drosophila genomic engineering.

    Science.gov (United States)

    Sebo, Zachary L; Lee, Han B; Peng, Ying; Guo, Yi

    2014-01-01

    The type II CRISPR/Cas9 system (clustered regularly interspaced short palindromic repeats/CRISPR-associated) has recently emerged as an efficient and simple tool for site-specific engineering of eukaryotic genomes. To improve its applications in Drosophila genome engineering, we simplified the standard two-component CRISPR/Cas9 system by generating a stable transgenic fly line expressing the Cas9 endonuclease in the germline (Vasa-Cas9 line). By injecting vectors expressing engineered target-specific guide RNAs into Vasa-Cas9 fly embryos, mutations were generated from site-specific DNA cleavages and efficiently transmitted into progenies. Because Cas9 endonuclease is the universal component of the type II CRISPR/Cas9 system, site-specific genomic engineering based on this improved platform can be achieved with lower complexity and toxicity, greater consistency, and excellent versatility.

  1. CRISPR/Cas9 Platforms for Genome Editing in Plants: Developments and Applications.

    Science.gov (United States)

    Ma, Xingliang; Zhu, Qinlong; Chen, Yuanling; Liu, Yao-Guang

    2016-07-06

    The clustered regularly interspaced short palindromic repeat (CRISPR)-associated protein9 (Cas9) genome editing system (CRISPR/Cas9) is adapted from the prokaryotic type II adaptive immunity system. The CRISPR/Cas9 tool surpasses other programmable nucleases, such as ZFNs and TALENs, for its simplicity and high efficiency. Various plant-specific CRISPR/Cas9 vector systems have been established for adaption of this technology to many plant species. In this review, we present an overview of current advances on applications of this technology in plants, emphasizing general considerations for establishment of CRISPR/Cas9 vector platforms, strategies for multiplex editing, methods for analyzing the induced mutations, factors affecting editing efficiency and specificity, and features of the induced mutations and applications of the CRISPR/Cas9 system in plants. In addition, we provide a perspective on the challenges of CRISPR/Cas9 technology and its significance for basic plant research and crop genetic improvement.

  2. Clustered regularly interspaced short palindromic repeat associated protein genes cas1 and cas2 in Shigella%志贺菌成簇规律间隔短回文重复序列相关蛋白基因cas1和cas2研究

    Institute of Scientific and Technical Information of China (English)

    薛泽润; 王颖芳; 段广才; 王鹏飞; 王琳琳; 郭向娇; 郗园林

    2014-01-01

    Objective To detect the distribution of clustered regularly interspaced short palindromic repeat(CRISPR)associated protein genes cas1 and cas2 in Shigella and to understand the characteristics of CRISPR with relationship between CRISPR and related characteristics on drug resistance. Methods CRISPR associated protein genes cas1 and cas2 in Shigella were detected by PCR,with its products sequenced and compared.Results The CRISPR-associated protein genes cas1 and cas2 were found in all the 196 Shigella isolates which were isolated at different times and locations in China. Consistencies showed through related sequencing appepared as follows:cas2,cas1 (a) and cas1(b)were 96.44%,97.61%and 96.97%,respectively. There were two mutations including 3177129 site(C→G)and 3177126 site(G→C)of cas1(b)gene in 2003135 strain which were not found in the corresponding sites of Z23 and 2008113. Results showed that in terms of both susceptibility and antibiotic-resistance,strain 2003135 was stronger than Z23 and 2008113. Conclusion CRISPR system widely existed in Shigella,with the level of drug resistance in cas1(b) gene mutant strains higher than in wild strains. Cas1(b)gene mutation might be one of the reasons causing the different levels of resistance.%目的:研究成簇规律间隔短回文重复序列(CRISPR)相关蛋白基因cas1和cas2在志贺菌中的分布,并分析cas1和cas2基因突变与细菌耐药的关系。方法采用PCR扩增196株志贺菌cas1和cas2基因。对3株志贺菌(Z23、2003135、2008113)的cas2、cas1(a)和cas1(b)基因进行测序,分析其突变与耐药之间的关系。结果196株志贺菌均检出CRISPR相关蛋白基因cas1和cas2。测序结果显示,cas2的一致性为96.44%,cas1(a)的一致性为97.61%,cas1(b)的一致性为96.97%。菌株2003135的cas1(b)基因有2个突变位点:3177129位点(C→G)及3177126位点(G→C),Z23、2008113对应位置没有突变

  3. Chemical and Biophysical Modulation of Cas9 for Tunable Genome Engineering.

    Science.gov (United States)

    Nuñez, James K; Harrington, Lucas B; Doudna, Jennifer A

    2016-03-18

    The application of the CRISPR-Cas9 system for genome engineering has revolutionized the ability to interrogate genomes of mammalian cells. Programming the Cas9 endonuclease to induce DNA breaks at specified sites is achieved by simply modifying the sequence of its cognate guide RNA. Although Cas9-mediated genome editing has been shown to be highly specific, cleavage events at off-target sites have also been reported. Minimizing, and eventually abolishing, unwanted off-target cleavage remains a major goal of the CRISPR-Cas9 technology before its implementation for therapeutic use. Recent efforts have turned to chemical biology and biophysical approaches to engineer inducible genome editing systems for controlling Cas9 activity at the transcriptional and protein levels. Here, we review recent advancements to modulate Cas9-mediated genome editing by engineering split-Cas9 constructs, inteins, small molecules, protein-based dimerizing domains, and light-inducible systems.

  4. Tun Dr. Mahathir’s Leadership Communication: The Confucian Perspective

    Directory of Open Access Journals (Sweden)

    Cheah Joyce Lynn-Sze

    2017-01-01

    Full Text Available Previous studies have provided valuable insights into the impact of culture on the concept of leadership communication. However, only a few studies that focused on exploring and understanding the values of Confucianism and its impact on the Chinese culture, especially in Malaysia. Thus, this study is among the first study to examine the leadership communication from the perspective of Confucianism towards the leadership of Dr. Mahathir from the perspective of the Chinese community in Malaysia. This study aims to assess the role of Confucian values in Dr. Mahathir’s leadership communication. This study interviewed 15 Chinese leaders. The findings reveal that three main Confucian values are relevant to Dr. Mahathir’s leadership communication. The Confucian values are Zhi (wisdom, Xin (trust and Xiao (filial piety. This study provides a new perspective on leadership communication from the context of cultural diversity in Malaysia which emphasizes on Confucian values.

  5. Nuclease activity of Legionella pneumophila Cas2 promotes intracellular infection of amoebal host cells.

    Science.gov (United States)

    Gunderson, Felizza F; Mallama, Celeste A; Fairbairn, Stephanie G; Cianciotto, Nicholas P

    2015-03-01

    Legionella pneumophila, the primary agent of Legionnaires' disease, flourishes in both natural and man-made environments by growing in a wide variety of aquatic amoebae. Recently, we determined that the Cas2 protein of L. pneumophila promotes intracellular infection of Acanthamoeba castellanii and Hartmannella vermiformis, the two amoebae most commonly linked to cases of disease. The Cas2 family of proteins is best known for its role in the bacterial and archeal clustered regularly interspaced short palindromic repeat (CRISPR)-CRISPR-associated protein (Cas) system that constitutes a form of adaptive immunity against phage and plasmid. However, the infection event mediated by L. pneumophila Cas2 appeared to be distinct from this function, because cas2 mutants exhibited infectivity defects in the absence of added phage or plasmid and since mutants lacking the CRISPR array or any one of the other cas genes were not impaired in infection ability. We now report that the Cas2 protein of L. pneumophila has both RNase and DNase activities, with the RNase activity being more pronounced. By characterizing a catalytically deficient version of Cas2, we determined that nuclease activity is critical for promoting infection of amoebae. Also, introduction of Cas2, but not its catalytic mutant form, into a strain of L. pneumophila that naturally lacks a CRISPR-Cas locus caused that strain to be 40- to 80-fold more infective for amoebae, unequivocally demonstrating that Cas2 facilitates the infection process independently of any other component encoded within the CRISPR-Cas locus. Finally, a cas2 mutant was impaired for infection of Willaertia magna but not Naegleria lovaniensis, suggesting that Cas2 promotes infection of most but not all amoebal hosts.

  6. Dr. Xie Haizhou's Experience in Treating Senile Dementia

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    @@ Dr. Xie Haizhou, a renowned TCM physician, comes from an aristocratic TCM family, and is now a distinguished research fellow in China Academy of Chinese Medical Sciences. Having been engaged in TCM clinical practice, scientific research and teaching for more than 60 years, Dr. Xie has obtained rich experience in the treatment of obstinate and miscellaneous diseases, especially diseases of the brain and spinal cord. The authors have been fortunate enough to study under him and benefited a lot from his experience. The following is a brief introduction to his experience in the treatment of senile dementia.

  7. Study of photoluminescence properties of thin films DR1

    Energy Technology Data Exchange (ETDEWEB)

    Herman,, E-mail: herman@fi.itb.ac.id; Ulya, Naily, E-mail: naily.ulya@gmail.com [Physics of Magnetism and Photonics Group, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung (Indonesia)

    2015-09-30

    Fabrication of thin films DR1 have been carried out by the EFA-PDF (Electric Field-Assisted Physical Vapor Deposition) method to obtain films with parallel dipole arrangement. Molecular deposition process is performed by applying an electric field that is placed between the substrate ITO (Indium Tin Oxide) and copper mesh. The resulting films were characterized by using a spectrofluorometer. Analysis of the emission spectrum, indicate that DR1 molecules in the film oriented perpendicular to the substrate and arranged in parallel order (H-aggregate). As an effect, the peak of the emission spectrum appears stronger along with the increase of electric field strength.

  8. Dr Jacob van der Land, marine biologist extraordinary

    OpenAIRE

    Bruggen, van, A.

    2001-01-01

    This contribution is an attempt to sketch the life and works of Dr Jacob van der Land, curator of worms and chief marine biologist of the National Museum of Natural History, on the occasion of his official retirement. Born in 1935, Jacob van der Land read biology at Leiden University (1958-1964), where he obtained his Ph.D. in 1970 on a treatise on the Priapulida under the supervision of Prof. Dr L.D. Brongersma. In 1964 he was appointed curator of worms in the museum. Later on he took over l...

  9. CRISPR/Cas9 Cleavage of Viral DNA Efficiently Suppresses Vaccinia Viruses%CRISPR/Cas9切割痘病毒DNA抑制病毒复制

    Institute of Scientific and Technical Information of China (English)

    王娇娇; 张新敏; 倪爱民; 章康健; 刘新垣; 刘锡君

    2016-01-01

    当前,CRISPR/Cas9系统靶向痘苗病毒的相关研究鲜有报道.该文采用了CRISPR/Cas9基因组定点编辑技术,以痘病毒为研究对象.在重组痘病-毒WR-EGFP中针对EGFP基因序列设计不同靶点gRNAs,转染Cas9/gRNA质粒,同时感染WR-EGFP痘病毒,随后观察EGFP荧光表达、提取病毒基因组,通过PCR进行切口鉴定并运用错配酶切法验-CRISPR/Cas9对痘病毒靶点DNA切割的切口修复情况.与单独感染WR-EGFP组相比,转染了Cas9/gRNA 174和Cas9/gRNA 175质粒的EGFP荧光强度明显降低,表明CRISPR/Cas9抑制WR-EGFP病毒中EGFP基因的表达.相对定量PCR及错配酶切实验结果显示,CRISPR/Cas9切割靶点导致WR-EGFP病毒基因组产生了切口,并且存在DNA错配修复现象.最后,通过结晶紫染色法测定痘病毒的滴度,结果显示,经CRISPR/Cas9处理组的痘病毒滴度显著下降,即CRISPR/Cas9能够使痘病毒的复制能力显著地降低.综上所述,CRISPR/Cas9切割痘病毒基因组DNA并且抑制痘病毒复制.

  10. Insights into the CRISPR/Cas system of Gardnerella vaginalis

    Directory of Open Access Journals (Sweden)

    Pleckaityte Milda

    2012-12-01

    Full Text Available Abstract Background Gardnerella vaginalis is identified as the predominant colonist of the vaginal tracts of women diagnosed with bacterial vaginosis (BV. G. vaginalis can be isolated from healthy women, and an asymptomatic BV state is also recognised. The association of G. vaginalis with different clinical phenotypes could be explained by different cytotoxicity of the strains, presumably based on disparate gene content. The contribution of horizontal gene transfer to shaping the genomes of G. vaginalis is acknowledged. The CRISPR loci of the recently discovered CRISPR/Cas microbial defence system provide a historical view of the exposure of prokaryotes to a variety of foreign genetic elements. Results The CRISPR/Cas loci were analysed using available sequence data from three G. vaginalis complete genomes and 18 G. vaginalis draft genomes in the NCBI database, as well as PCR amplicons of the genomic DNA of 17 clinical isolates. The cas genes in the CRISPR/Cas loci of G. vaginalis belong to the E. coli subtype. Approximately 20% of the spacers had matches in the GenBank database. Sequence analysis of the CRISPR arrays revealed that nearly half of the spacers matched G. vaginalis chromosomal sequences. The spacers that matched G. vaginalis chromosomal sequences were determined to not be self-targeting and were presumably neither constituents of mobile-element-associated genes nor derived from plasmids/viruses. The protospacers targeted by these spacers displayed conserved protospacer-adjacent motifs. Conclusions The CRISPR/Cas system has been identified in about one half of the analysed G. vaginalis strains. Our analysis of CRISPR sequences did not reveal a potential link between their presence and the virulence of the G. vaginalis strains. Based on the origins of the spacers found in the G. vaginalis CRISPR arrays, we hypothesise that the transfer of genetic material among G. vaginalis strains could be regulated by the CRISPR/Cas mechanism. The

  11. STATISTICAL STUDY OF 2XMMi-DR3/SDSS-DR8 CROSS-CORRELATION SAMPLE

    Energy Technology Data Exchange (ETDEWEB)

    Zhang Yanxia; Zhou Xinlin; Zhao Yongheng [Key Laboratory of Optical Astronomy, National Astronomical Observatories, Chinese Academy of Sciences, 20A Datun Road, Chaoyang District, 100012 Beijing (China); Wu Xuebing, E-mail: zyx@bao.ac.cn [Department of Astronomy, Peking University, 100871 Beijing (China)

    2013-02-01

    Cross-correlating the XMM-Newton 2XMMi-DR3 catalog with the Sloan Digital Sky Survey (SDSS) Data Release 8, we obtain one of the largest X-ray/optical catalogs and explore the distribution of various classes of X-ray emitters in the multidimensional photometric parameter space. Quasars and galaxies occupy different zones while stars scatter in them. However, X-ray active stars have a certain distributing rule according to spectral types. The earlier the type of stars, the stronger its X-ray emitting. X-ray active stars have a similar distribution to most stars in the g - r versus r - i diagram. Based on the identified samples with SDSS spectral classification, a random forest algorithm for automatic classification is performed. The result shows that the classification accuracy of quasars and galaxies adds up to more than 93.0% while that of X-ray emitting stars only amounts to 45.3%. In other words, it is easy to separate quasars and galaxies, but it is difficult to discriminate X-ray active stars from quasars and galaxies. If we want to improve the accuracy of automatic classification, it is necessary to increase the number of X-ray emitting stars, since the majority of X-ray emitting sources are quasars and galaxies. The results obtained here will be used for the optical spectral survey performed by the Large sky Area Multi-Object fiber Spectroscopic Telescope (LAMOST, also named the Guo Shou Jing Telescope), which is a Chinese national scientific research facility operated by the National Astronomical Observatories, Chinese Academy of Sciences.

  12. Psychology in Japan.

    Science.gov (United States)

    Imada, Hiroshi; Tanaka-Matsumi, Junko

    2016-06-01

    The purpose of this article is to provide information about Japan and its psychology in advance of the 31st International Congress of Psychology (ICP), to be held in Yokohama, Japan, in 2016. The article begins with the introduction of the Japanese Psychological Association (JPA), the hosting organization of the ICP 2016, and the Japanese Union of Psychological Associations consisting of 51 associations/societies, of which the JPA is a member. This is followed by a brief description of a history of psychology of Japan, with emphasis on the variation in our approach to psychology in three different periods, that is, the pre- and post-Pacific War periods, and the post-1960 period. Next, the international contributions of Japanese psychology/psychologists are discussed from the point of view of their visibility. Education and training in psychology in Japanese universities is discussed with a final positive remark about the long-awaited enactment of the Accredited Psychologist Law in September, 2015.

  13. IFE PsychologIA

    African Journals Online (AJOL)

    Influence of Parenting Styles on Psychological Well-Being and School Adjustment of ... Aviation Psychological Practice in the Nigerian Air Force: Challenges and ... Psychosocial Predictors of Quality of Life among Caregivers of Chronically Ill ...

  14. Personality theory, abnormal psychology, and psychological measurement. A psychological behaviorism.

    Science.gov (United States)

    Staats, A W

    1993-01-01

    Behaviorism, because it has not had a theory of personality, has been separated from the rest of psychology, unable in large part to draw from or contribute to it. Traditional psychology has not had a theory of personality that says what personality is, how it comes about, or how it functions. An antagonism has resulted that weakens rather than complements each tradition. Psychological behaviorism presents a new type of theory of personality. Derived from experimentation, it is constructed from basic theories of emotion, language, and sensory-motor behavior. It says personality is composed of learned basic behavioral repertoires (BBRs) that affect behavior. Personality measurement instruments are analyzed in terms of the BBRs, beginning the behaviorization of this field and calling for much additional research. These multilevel developments are then basic in psychological behaviorism's theory of abnormal behavior and of clinical treatment. The approach opens many new avenues of empirical and theoretical work.

  15. Psychology of Religion

    OpenAIRE

    Ulu, Mustafa

    2014-01-01

    In this chapter of book that entitled Science, Religion and Society: An Encyclopedia of History, Culture, and Controversy has been given important informations about psychology of religion’s historical development as well as pioneer figures’ contributions. In this text some evaluations has been made by categorizing studies in field of psychology of religion. Finally some informations are provided about current status of the psychology of religion and position of the psychology of religion ...

  16. Logotherapy and positive psychology

    OpenAIRE

    Oscar R. Oro

    2015-01-01

    Psychology omitted to approach, during almost a century, the positive aspects from persons, like creativity, humor, optimism, hope, forgiveness, life meaning, and happiness. These themes are approached by Positive Psychology, with Seligman like the principal exponent. Psychology was dedicated to explore the negative aspects from human beings improving human health. Nevertheless, this pathogenic model could not prevent mental disease. Concepts of Positive Psychology have a solid antecedent in ...

  17. Dr. Long Wenjun's Experience in Auriculo-Acupuncture

    Institute of Scientific and Technical Information of China (English)

    Tian Yongping; Hou Chunying

    2007-01-01

    @@ Having been engaged in TCM practice for more than 40 years, Dr. Long Wenjun from Gansu College of Traditional Chinese Medicine is very good at treating acute infectious diseases, acute painful diseases and certain obstinate diseases with auriculo-acupuncture. Following is an introduction to his experience with some sample cases.

  18. Keeping the Dream Alive. Dr. Martin Luther King, Jr.

    Science.gov (United States)

    Cassells, Linda; And Others

    1993-01-01

    Presents activities for teaching elementary students about Dr. Martin Luther King's life, the civil rights movement, and King's accomplishments. Suggestions include shoebox dioramas, acronym games, oral histories, multicultural awareness activities, pledge cards, community service projects, special commemorative ceremonies, and a book of dreams.…

  19. Dr.Xuan Guowei's Experience in Treating Dermatosis

    Institute of Scientific and Technical Information of China (English)

    刘爱民

    2004-01-01

    @@ Dr. Xuan Guowei has been engaged in medical practice, teaching and scientific research on dermatosis for nearly 40 years, and is famous for his meticulous scholarship and outstanding art of healing. The following are some examples of his unique academic ideas and creative clinical practice.

  20. HLA-DR expression and disease activity in ulcerative colitis

    DEFF Research Database (Denmark)

    Poulsen, L O; Elling, P; Sørensen, Flemming Brandt

    1986-01-01

    In 12 patients with active ulcerative colitis (UC) the rectal epithelial cells were analyzed for HLA-DR antigens by an immunohistochemical technique. The clinical, rectoscopic, and histologic stages were also determined. The investigations were carried out at the beginning of the study and 2 weeks...

  1. Dr.Zhu Hongming's Experience in Treating Chronic Gastritis

    Institute of Scientific and Technical Information of China (English)

    朱传伟

    2004-01-01

    @@ Dr. Zhu Hongming has engaged in TCM internal medicine for more than 40 years and accumulated rich clinical experience, especially in the treatment of diseases of the spleen and stomach. The following is a brief introduction to his experience in TCM differential treatment of chronic gastritis.

  2. Obituary Dr A. W. Kloos (1880—1952)

    NARCIS (Netherlands)

    Ooststroom, van S.J.

    1952-01-01

    At the end of the previous number of “Blumea” could just be inserted the death notice of one of the honorary collaborators of the Rijksherbarium, Dr Ir A. W. Kloos, who passed away in his home at Dordrecht on June 3rd, 1952. A more detailed obituary may follow here. Abraham Willem Kloos was born at

  3. Shepard Award Winners, Part 1: Dr. Stephen Thacker

    Centers for Disease Control (CDC) Podcasts

    2009-07-22

    This podcast highlights the accomplishments of Dr. Stephen Thacker, recipient of the prestigious 2009 CDC Charles C. Shepard Award.  Created: 7/22/2009 by Centers for Disease Control and Prevention (CDC).   Date Released: 7/22/2009.

  4. Shepard Award Winners, Part 3: Dr. Larissa Roux

    Centers for Disease Control (CDC) Podcasts

    2009-09-22

    This podcast highlights the accomplishments of Dr. Larissa Roux, recipient of the prestigious 2009 CDC Charles C. Shepard Award.  Created: 9/22/2009 by Centers for Disease Control and Prevention (CDC).   Date Released: 9/22/2009.

  5. Shepard Award Winners, Part 2: Dr. Tracie Williams

    Centers for Disease Control (CDC) Podcasts

    2009-07-29

    This podcast highlights the accomplishments of Dr. Tracie Williams, recipient of the prestigious 2009 CDC Charles C. Shepard Award.  Created: 7/29/2009 by Centers for Disease Control and Prevention (CDC).   Date Released: 7/29/2009.

  6. Dr Jacob van der Land, marine biologist extraordinary

    NARCIS (Netherlands)

    Bruggen, van A.C.

    2001-01-01

    This contribution is an attempt to sketch the life and works of Dr Jacob van der Land, curator of worms and chief marine biologist of the National Museum of Natural History, on the occasion of his official retirement. Born in 1935, Jacob van der Land read biology at Leiden University (1958-1964), wh

  7. Professor Oberth and Dr. von Braun at ARS Banquet

    Science.gov (United States)

    1961-01-01

    Dr. Wernher von Braun holds the coveted Hermarn Oberth award presented to him by Professor Oberth during the banquet hosted by the Alabama Section of the American Rocket Society (ARS), on October 19, 1961. The Oberth award was given for outstanding technical contributions to the field of astronautics or for the promotion and advancement of astronautical sciences.

  8. Dr. Yi Juqing's Experience in Treating Female Infertility

    Institute of Scientific and Technical Information of China (English)

    Meng Jimin

    2006-01-01

    @@ With rich experience accumulated in medical practice for 40 years, Dr. Yi Juqing (易菊清), a chief physician, is good at treating various kinds of refractory diseases. The following is an introduction of his experience in treating female infertility.

  9. Oxygen and Metastasis: A Conversation with Dr. Nick Restifo

    Science.gov (United States)

    Dr. Nick Restifo, a senior investigator in NCI’s Center for Cancer Research, discusses his recently published study finding that Oxygen, a molecule necessary for life, paradoxically aids cancer metastasis to the lung by impairing cancer-killing immune cells.

  10. Dr. Martin Luther King: A Unit for Primary Grades.

    Science.gov (United States)

    Sweet, Kelli

    1986-01-01

    Presents a unit consisting of 4 lessons which focus on Dr. Martin Luther King, Jr.'s childhood, his adult life and family, his civil rights work, and the national holiday which commemorates his birthday. Each lesson features specific objectives, learning activities, and instructional guidelines for the teacher. (TRS)

  11. The SDSS DR6 Luminosity Functions of Galaxies

    CERN Document Server

    Montero-Dorta, Antonio D

    2008-01-01

    We present number counts, luminosity functions (LFs) and luminosity densities of galaxies obtained using the Sloan Digital Sky Survey Sixth Data Release in all SDSS photometric bands. Thanks to the SDSS DR6, galaxy statistics have increased by a factor of ~9 in the u-band and by a factor of ~4-5 in the rest of the SDSS bands with respect to the previous work of Blanton et al. (2003b). In addition, we have achieved a high redshift completeness in our galaxy samples. Firstly, by making use of the survey masks, provided by the NYU-VAGC DR6, we have been able to define an area on the sky of high angular redshift completeness. Secondly, we guarantee that brightness-dependent redshift incompleteness is small within the magnitude ranges that define our galaxy samples. With these advances, we have estimated very accurate SDSS DR6 LFs in both the bright and the faint end. In the {0.1}^r-band, our SDSS DR6 luminosity function is well fitted by a Schechter LF with parameters Phi_{*}=0.90 +/- 0.07$, M_{*}-5log_{10}h=-20....

  12. Dr. Feng Xinghua's Experience in Treating Ankylosing Spondylitis

    Institute of Scientific and Technical Information of China (English)

    Liu Hongxiao

    2006-01-01

    @@ Ankylosing spondylitis (AS) is a rheumatic disease mainly involving the axial joints, characterized by sacro-iliilis and rigid spine. Dr. Feng Xinghua from Guang'anmen Hospital affiliated to China Academy of Chinese Medical Sciences has been engaged in the clinical research and treatment of arthralgia syndrome for more than 30 years. His experience is summarized as follows.

  13. Another Use for Dr. Seuss: Reading for Social Skills

    Science.gov (United States)

    Wolf, Janice J.; Baker, Pamela Hudson

    2012-01-01

    Time is limited. Teachers often struggle to address the diverse needs of learners in their classrooms, especially when faced with academic and social skills concerns. Finding effective ways to teach social skills in the context of academic instruction makes sense. Books by Dr. Seuss provide a variety of high-interest stories that can be used to…

  14. Celebrate the legacy of Dr. Martin Luther King Jr.

    OpenAIRE

    Lazenby, Jenna

    2006-01-01

    In celebration of the life and legacy of Dr. Martin Luther King, Jr., Virginia Tech will host a variety of educational and cultural programs and community service events Jan. 14-19, 2007. All events are free and open to the public.

  15. HLA-DR expression and disease activity in ulcerative colitis

    DEFF Research Database (Denmark)

    Poulsen, L O; Elling, P; Sørensen, Flemming Brandt

    1986-01-01

    In 12 patients with active ulcerative colitis (UC) the rectal epithelial cells were analyzed for HLA-DR antigens by an immunohistochemical technique. The clinical, rectoscopic, and histologic stages were also determined. The investigations were carried out at the beginning of the study and 2 weeks...

  16. Dr. Vernon W. Hughes, 81, authority on the subatomic

    CERN Multimedia

    Lavietes, S

    2002-01-01

    "Dr. Vernon W. Hughes, a Yale physicist whose investigation of particles called muons poked holes in standard subatomic theory and provided evidence for the existence of previously undetected matter, died at Yale-New Haven Hospital last Tuesday" (1/2 page).

  17. Frau Dr Anna Weber—van Bosse, neunzig Jahre

    NARCIS (Netherlands)

    Westerdijk, Johanna

    1942-01-01

    In Eerbeek, in einem der schön gelegenen Dörfer der Provinz Gelderland, steht ein altes Schloss, von der Verkehrsstrasse weit entfernt, und am Bach entlang zu erreichen. Es ist das sogenannte ”Huis Eerbeek“, das Haus der Frau Dr. Weber, die Herrin im wahren Sinne des Wortes. Da können wir heute der

  18. In memoriam dr. Anne van Wijngaarden (1925-2004)

    NARCIS (Netherlands)

    Broekhuizen, S.; Laar, van V.

    2005-01-01

    Op 4 oktober 2004 overleed Dr. Anne van Wijngaarden op 78-jarige leeftijd in zijn huis bij Millac- Carlux, Frankrijk. Hij was een van de Nederlandse oprichters van de Vereniging voor Zoogdierkunde en Zoogdierbescherming. Nadat zijn eindexamen op de middelbare school wilde Anne aanvankelijk geologie

  19. Dr Jacob van der Land, marine biologist extraordinary

    NARCIS (Netherlands)

    Bruggen, van A.C.

    2001-01-01

    This contribution is an attempt to sketch the life and works of Dr Jacob van der Land, curator of worms and chief marine biologist of the National Museum of Natural History, on the occasion of his official retirement. Born in 1935, Jacob van der Land read biology at Leiden University (1958-1964),

  20. Dr. Edward de Bono's Six Thinking Hats and Numeracy

    Science.gov (United States)

    Paterson, Anne

    2006-01-01

    In education, the term "metacognition" describes thinking about thinking. Within mathematics, the term "metacomputation" describes thinking about computational methods and tools. This article shows how Dr. Edward de Bono's Six Thinking Hats can be used to demonstrate metacognition and metacomputation in the primary classroom. The article suggests…

  1. Celebrate the legacy of Dr. Martin Luther King Jr.

    OpenAIRE

    Lazenby, Jenna

    2006-01-01

    In celebration of the life and legacy of Dr. Martin Luther King, Jr., Virginia Tech will host a variety of educational and cultural programs and community service events Jan. 14-19, 2007. All events are free and open to the public.

  2. Dr. Martin Luther King, Jr. Washington State Resource Guide.

    Science.gov (United States)

    Washington Office of the State Superintendent of Public Instruction, Olympia, WA. Div. of Instructional Programs and Services.

    The Washington State Resource Guide on Martin Luther King, Jr., supplies a wide variety of materials for use with all grade levels in classroom and assembly presentations in public schools. The goal is for every child enrolled in Washington State schools to learn about Dr. King during the days of January 15 to January 17. Resolutions supporting an…

  3. A Dr. Martin Luther King, Jr. Curriculum: Playing the Dream.

    Science.gov (United States)

    Clemens, Sydney Gurewitz

    1988-01-01

    Discusses curriculum for young children centered around the beliefs and teachings of Dr. Martin Luther King, Jr. His works are interpreted in a human rights context in which children find their voice in the peaceable resolution of everyday conflicts. Describes the Child of the Day program. (Author/RWB)

  4. Improving College Students English Learning with Dr. Eye Android Mid

    Science.gov (United States)

    Yang, Ju Yin; Che, Pei-Chun

    2015-01-01

    This paper investigates college students' English language learning through use of Dr. Eye Android handheld mobile Internet device (MID). Compared to related studies, students' English learning using MIDs has not been evaluated and fully understood in the field of higher education. Quantitatively, the researchers used TOEIC pretest and posttest to…

  5. Velocity Curve Studies of Spectroscopic Binary Stars V380 Cygni, V401 Cyg, V523 Cas, V373 Cas and V2388 Oph

    Indian Academy of Sciences (India)

    K. Karami; R. Mohebi

    2009-09-01

    Using measured radial velocity data of five double lined spectroscopic binary systems V380 Cygni, V401 Cyg, V523 Cas, V373 Cas and V2388 Oph, we find corresponding orbital and spectroscopic elements via the method introduced by Karami & Mohebi (2007) and Karami & Teimoorinia (2007). Our numerical results are in good agreement with those obtained by others using more traditional methods.

  6. An Effective Strategy for Reliably Isolating Heritable and Cas9-Free Arabidopsis Mutants Generated by CRISPR/Cas9-Mediated Genome Editing.

    Science.gov (United States)

    Gao, Xiuhua; Chen, Jilin; Dai, Xinhua; Zhang, Da; Zhao, Yunde

    2016-07-01

    Mutations generated by CRISPR/Cas9 in Arabidopsis (Arabidopsis thaliana) are often somatic and are rarely heritable. Isolation of mutations in Cas9-free Arabidopsis plants can ensure the stable transmission of the identified mutations to next generations, but the process is laborious and inefficient. Here, we present a simple visual screen for Cas9-free T2 seeds, allowing us to quickly obtain Cas9-free Arabidopsis mutants in the T2 generation. To demonstrate this in principle, we targeted two sites in the AUXIN-BINDING PROTEIN1 (ABP1) gene, whose function as a membrane-associated auxin receptor has been challenged recently. We obtained many T1 plants with detectable mutations near the target sites, but only a small fraction of T1 plants yielded Cas9-free abp1 mutations in the T2 generation. Moreover, the mutations did not segregate in Mendelian fashion in the T2 generation. However, mutations identified in the Cas9-free T2 plants were stably transmitted to the T3 generation following Mendelian genetics. To further simplify the screening procedure, we simultaneously targeted two sites in ABP1 to generate large deletions, which can be easily identified by PCR. We successfully generated two abp1 alleles that contained 1,141- and 711-bp deletions in the ABP1 gene. All of the Cas9-free abp1 alleles we generated were stable and heritable. The method described here allows for effectively isolating Cas9-free heritable CRISPR mutants in Arabidopsis.

  7. Broadening Staphylococcus aureus Cas9 Targeting Range by Modifying PAM Recognition

    Science.gov (United States)

    Kleinstiver, Benjamin P.; Prew, Michelle S.; Tsai, Shengdar Q.; Nguyen, Nhu T.; Topkar, Ved V.; Zheng, Zongli; Joung, J. Keith

    2015-01-01

    CRISPR-Cas9 nucleases are primarily guided by RNA-DNA interactions but also require Cas9-mediated recognition of a protospacer adjacent motif (PAM). While potentially advantageous for specificity, extended PAM sequences limit the targeting range of Cas9 orthologues for genome editing. One possible strategy to relieve this restriction is to relax specificities for certain positions within the PAM. Here we used molecular evolution to modify the NNGRRT PAM specificity of Staphylococcus aureus Cas9 (SaCas9). One variant we identified, referred to as KKH SaCas9, shows robust genome editing activities at endogenous human target sites with NNNRRT PAMs. Importantly, using GUIDE-seq, we show that both wild-type and KKH SaCas9 induce comparable numbers of off-target effects in human cells. KKH SaCas9 increased the targeting range of SaCas9 by nearly two- to four-fold. Our molecular evolution strategy does not require structural information and therefore should be applicable to a wide range of Cas9 orthologues. PMID:26524662

  8. RNA-guided transcriptional regulation in planta via synthetic dCas9-based transcription factors

    KAUST Repository

    Piatek, Agnieszka Anna

    2014-11-14

    Targeted genomic regulation is a powerful approach to accelerate trait discovery and development in agricultural biotechnology. Bacteria and archaea use clustered regularly interspaced short palindromic repeats (CRISPRs) and CRISPR-associated (Cas) regulatory systems for adaptive molecular immunity against foreign nucleic acids introduced by invading phages and conjugative plasmids. The type II CRISPR/Cas system has been adapted for genome editing in many cell types and organisms. A recent study used the catalytically inactive Cas9 (dCas9) protein combined with guide-RNAs (gRNAs) as a DNA-targeting platform to modulate gene expression in bacterial, yeast, and human cells. Here, we modified this DNA-targeting platform for targeted transcriptional regulation in planta by developing chimeric dCas9-based transcriptional activators and repressors. To generate transcriptional activators, we fused the dCas9 C-terminus with the activation domains of EDLL and TAL effectors. To generate a transcriptional repressor, we fused the dCas9 C-terminus with the SRDX repression domain. Our data demonstrate that dCas9 fusion with the EDLL activation domain (dCas9:EDLL) and the TAL activation domain (dCas9:TAD), guided by gRNAs complementary to selected promoter elements, induce strong transcriptional activation on Bs3

  9. CRISPR-Cas adaptive immune systems of the sulfolobales

    DEFF Research Database (Denmark)

    Garrett, Roger Antony; Shah, Shiraz Ali; Erdmann, Susanne

    2015-01-01

    The Sulfolobales have provided good model organisms for studying CRISPR-Cas systems of the crenarchaeal kingdom of the archaea. These organisms are infected by a wide range of exceptional archaea-specific viruses and conjugative plasmids, and their CRISPR-Cas systems generally exhibit extensive...... structural and functional diversity. They carry large and multiple CRISPR loci and often multiple copies of diverse Type I and Type III interference modules as well as more homogeneous adaptation modules. These acidothermophilic organisms have recently provided seminal insights into both the adaptation...... process, the diverse modes of interference, and their modes of regulation. The functions of the adaptation and interference modules tend to be loosely coupled and the stringency of the crRNA-DNA sequence matching during DNA interference is relatively low, in contrast to some more streamlined CRISPR...

  10. Application of CRISPR/Cas9 Technology to HBV

    Directory of Open Access Journals (Sweden)

    Guigao Lin

    2015-11-01

    Full Text Available More than 240 million people around the world are chronically infected with hepatitis B virus (HBV. Nucleos(tide analogs and interferon are the only two families of drugs to treat HBV currently. However, none of these anti-virals directly target the stable nuclear covalently closed circular DNA (cccDNA, which acts as a transcription template for viral mRNA and pre-genomic RNA synthesis and secures virus persistence. Thus, the fact that only a small number of patients treated achieve sustained viral response (SVR or cure, highlights the need for new therapies against HBV. The clustered regularly interspaced short palindromic repeats (CRISPR/Cas9 gene editing system can specifically target the conserved regions of the HBV genome. This results in robust viral suppression and provides a promising tool for eradicating the virus. In this review, we discuss the function and application of the CRISPR/Cas9 system as a novel therapy for HBV.

  11. Maladie de Haglund: à propos de trois cas

    OpenAIRE

    Adigo, Amégninou Mawuko Yao; Gnakadja, Néille Gbèssi; Dellanh, Yaovi Yanick; Adambounou, Kokou; Djagnikpo, Oni; Agoda-Kousséma, Lama Kegdigoma; Adoko, Abikou Léon; Adjénou, Komlanvi Victor

    2015-01-01

    La maladie de Haglund est une pathologie relativement sous évaluée. Elle est liée à un conflit calcanéo-achilléen. Nous rapportons les cas de patients âgés de 40, 42 et 37 ans, révélés par des œdèmes douloureux de la cheville. Le diagnostic a été confirmé à la radiographie standard de la cheville en charge et à l’échographie chez tous les patients. Un seul patient avait bénéficié d'une exploration IRM. Le traitement, initialement médical dans tous les cas, s'est soldé par une chirurgie de rés...

  12. CAS - CERN Accelerator School: Specialised course on Magnets

    CERN Document Server

    CAS 2009

    2010-01-01

    These proceedings present the lectures given at the twenty-third specialized course organized by the CERN Accelerator School (CAS), the topic being 'Magnets'. The course was held in Bruges, Belgium, from 16 to 25 June 2009. This is the first time this topic has been selected for a specialized course. Taking into account the number of related applications currently in use in accelerators around the world, but, even more important, the worrying decrease in the corresponding expertise in the different laboratories, it was recognized that such a topic should definitively be incorporated into the CAS series of specialized courses. The specific aim of the course was to introduce the participants to the basics of resistive magnet design and its underlying theoretical concepts. The first part of the school dealt with basic introductory courses such as Maxwell's equations for magnets, beam optics, physics and measurement of magnetic materials, the different types of resistive magnets and their respective performance, ...

  13. Adapting CRISPR/Cas9 for functional genomics screens.

    Science.gov (United States)

    Malina, Abba; Katigbak, Alexandra; Cencic, Regina; Maïga, Rayelle Itoua; Robert, Francis; Miura, Hisashi; Pelletier, Jerry

    2014-01-01

    The use of CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated protein) for targeted genome editing has been widely adopted and is considered a "game changing" technology. The ease and rapidity by which this approach can be used to modify endogenous loci in a wide spectrum of cell types and organisms makes it a powerful tool for customizable genetic modifications as well as for large-scale functional genomics. The development of retrovirus-based expression platforms to simultaneously deliver the Cas9 nuclease and single guide (sg) RNAs provides unique opportunities by which to ensure stable and reproducible expression of the editing tools and a broad cell targeting spectrum, while remaining compatible with in vivo genetic screens. Here, we describe methods and highlight considerations for designing and generating sgRNA libraries in all-in-one retroviral vectors for such applications.

  14. Towards Developing a CAS E-Journal Archiving System

    Institute of Scientific and Technical Information of China (English)

    ZHANG ZHIXIONG; WU ZHENXIN; ZHAO QI; LIU JIANHUA; GUO WENLI; XU JIAN

    2008-01-01

    As a part of NSTL proposed long-term preservation of digital information network,the National Science Library of CAS(NSLC)constructed a long-term preservation system of electronic journals called CAS E-Journal Archiving System,which was based on OAIS and adapted Fedora as the fundament system.The system chose a certain amount of electronic journals as pilot resources.Through three major system functional components comprising ingest,preservation management and data dissemination,the system took the long-term preservation of digital objects function into practice.The system is designed to be open and scalable.It realized digital objects management and lifecycle management.In this paper we describe the design of the system,and the issues we have learnt about long-term preservation.

  15. MPS/CAS Partner Group on Nanostructured Materials

    Institute of Scientific and Technical Information of China (English)

    Lu Ke; Manfred Rühle

    2004-01-01

    @@ As one of the first Partner Groups which were initiated to foster scientific exchange and interaction between the Max Pianck Society (MPS) and the Chinese Academy of Sciences (CAS), the Partner Group led by Lu Ke was established on April 1, 1999.During the past five years, the group has received substantial support from the CAS for equipment and from the MPS for personnel and travel expenses. Extensive and productive collaborations between the research staff and students of the Partner Group with several professors (departments) in the Max Planck Institute for Metals Research (MPI-MF) have led to significant advances in synthesis,mechanical properties, melting and superheating of nanostructured metals. The research is well recognized internationally and a substantial number of publications in high quality, peer-reviewed journals (including Science and Physical Review Letters) have resulted.

  16. Energy biotechnology in the CRISPR-Cas9 era.

    Science.gov (United States)

    Estrela, Raissa; Cate, Jamie Harrison Doudna

    2016-04-01

    The production of bioenergy from plant biomass previously relied on using microorganisms that rapidly and efficiently convert simple sugars into fuels and chemicals. However, to exploit the far more abundant carbon fixed in plant cell walls, future industrial production hosts will need to be engineered to leverage the most efficient biochemical pathways and most robust traits that can be found in nature. The CRISPR-Cas9 genome editing technology now enables writing the genome at will, which will allow biotechnology to become an 'information science.' This review covers recent advances in using CRISPR-Cas9 to engineer the genomes of a wide variety of organisms that could be use in the industrial production of biofuels and renewable chemicals.

  17. Dynamic Star Formation in the Massive DR21 Filament

    Energy Technology Data Exchange (ETDEWEB)

    Schneider, N.; /Saclay; Csengeri, T.; /Saclay; Bontemps, S.; /OASU, Floirac; Motte, F.; /Saclay; Simon, R.; /Cologne U.; Hennebelle, P.; /Paris Observ.; Federrath, C.; /ZAH, Heidelberg; Klessen, R.; /ZAH, Heidelberg /KIPAC, Menlo Park

    2010-08-25

    The formation of massive stars is a highly complex process in which it is unclear whether the star-forming gas is in global gravitational collapse or an equilibrium state supported by turbulence and/or magnetic fields. By studying one of the most massive and dense star-forming regions in the Galaxy at a distance of less than 3 kpc, i.e. the filament containing the well-known sources DR21 and DR21(OH), we attempt to obtain observational evidence to help us to discriminate between these two views. We use molecular line data from our {sup 13}CO 1 {yields} 0, CS 2 {yields} 1, and N{sub 2}H{sup +} 1 {yields} 0 survey of the Cygnus X region obtained with the FCRAO and CO, CS, HCO{sup +}, N{sub 2}H{sup +}, and H{sub 2}CO data obtained with the IRAM 30m telescope. We observe a complex velocity field and velocity dispersion in the DR21 filament in which regions of the highest column-density, i.e., dense cores, have a lower velocity dispersion than the surrounding gas and velocity gradients that are not (only) due to rotation. Infall signatures in optically thick line profiles of HCO{sup +} and {sup 12}CO are observed along and across the whole DR21 filament. By modelling the observed spectra, we obtain a typical infall speed of {approx}0.6 km s{sup -1} and mass accretion rates of the order of a few 10{sup -3} M{sub {circle_dot}} yr{sup -1} for the two main clumps constituting the filament. These massive clumps (4900 and 3300 M{sub {circle_dot}} at densities of around 10{sup 5} cm{sup -3} within 1 pc diameter) are both gravitationally contracting. The more massive of the clumps, DR21(OH), is connected to a sub-filament, apparently 'falling' onto the clump. This filament runs parallel to the magnetic field. Conclusions. All observed kinematic features in the DR21 filament (velocity field, velocity dispersion, and infall), its filamentary morphology, and the existence of (a) sub-filament(s) can be explained if the DR21 filament was formed by the convergence of flows

  18. Psychology in India

    Science.gov (United States)

    Sushma, B.; Padmaja, G.

    2011-01-01

    Psychology forms the basis of every human activity. The scope of psychology is increasingly widening in various economic, political, social, cultural and technological aspects. Though the application of psychology is extending to various aspects of life, it needs to be indigenised to address the dynamic needs in the various socio-economic contexts…

  19. Psychology in Mexico

    Science.gov (United States)

    Ruiz, Eleonora Rubio

    2011-01-01

    The first formal psychology course taught in Mexico was in 1896 at Mexico's National University; today, National Autonomous University of Mexico (UNAM in Spanish). The modern psychology from Europe and the US in the late 19th century were the primary influences of Mexican psychology, as well as psychoanalysis and both clinical and experimental…

  20. Psychology and Literature.

    Science.gov (United States)

    Lester, David

    1987-01-01

    Psychology and literature focus on human behavior. There are several points where the interests of psychologists and literary scholars converge. This convergence is evident in the use of literature to test psychological theories and to understand human behavior in historical times, in the psychological analyses of literature, and in psychological…

  1. Humanistic Psychology: How Realistic?

    Science.gov (United States)

    Riebel, Linda

    1982-01-01

    Overviews themes relating to humanistic psychology. Discusses the tendency of theorists to unconsciously externalize their own psyches. Examines the historical context of humanistic psychology. Discusses humanistic psychology's contribution to understanding the less healthy person. Provides instances of unrealistic thinking by humanistic…

  2. Intro through Internet Psychology.

    Science.gov (United States)

    Webster, Sandra K.; Kelliher, Thomas P.

    Psychology and computer science were clustered into a course in "Internet Psychology" with the goal of enabling students to use electronic networks responsibly and creatively and to understand the principles of psychology as they operate in the electronic context. Fourteen students from a variety of majors registered for the class.…

  3. The DR3(w18),DQw4 Haplotype Differs from DR3(w17),DQw2 Haplotypes at Multiple Class II Loci

    Science.gov (United States)

    1989-01-01

    DR3, members of the DRw52 family, are especially important because of the association of DR3 with a number of autoimmune diseases such as insulin...dependent diabetes, myasthenia gravis, and Graves’ disease [13]. We have begun a study of a DR3 haplotype, DR3(w 18), commonly found in the American...molecules. Proc Nail Acad Sci USA 83:3968, 1986. 5. Ball E, Stastny P: Antigen specific HLA-restriction determinant distinct from HLA-DR. Immunogenetics

  4. Collision Avoidance System (CAS): Human Factors Engineering Evaluation.

    Science.gov (United States)

    1982-12-01

    personnel indicated that the CAS console was much too big for the limited amount of space available on RANGER’s bridge. The console is 40 inches wide...avoidance (C/A) data. > RANGE * 12//2 < (T >~ LOG SPEED 4 10.0 KT < E1 i >~ HEADING 4 270 DEG * < J [ > BRGCRSR * 000 DEG4C/ ADATA < - > TRIAL SPD 4 0 <EJ

  5. Flagship project on cryopheric changes launched at CAS

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    @@ With the support of the National Basic Research Program (dubbed "973" Program), a research project entitled the "dynamic process of China's cryosphere, and the mechanism behind its impact on climate, hydrology and ecology" has recently been launched at the CAS Cold and Arid Regions Environmental and Engineering Research Institute (CAREERI) in Lanzhou, capital of northwest China's Gansu Province, marking the inception of a systematic and all-round exploration of the subject.

  6. CAS Institute of Information Engineering sees a good start

    Institute of Scientific and Technical Information of China (English)

    2012-01-01

    The Institute of Information Engineering (liE), a new institute under CAS approved for establishment in 2011, has made a good start in its development in a recent year, By far, it has set up several statelevel innovation platforms, including the State Key Laboratory of Information Security, the State Engineering Laboratory of Information Contenl. Security Technology, the National Engineering Research Center of Information Security, and the

  7. CAS Will Attend the 11th IFAN International Conference

    Institute of Scientific and Technical Information of China (English)

    2004-01-01

    @@ IFAN (The International Federation of Standards Users), together with NKN (Normalisatie Kringen Nederland) and NEN (Nederlands Normalisatie-institute), will organize the 11th IFAN International Conference on the subject of "Standardization-Awareness-Compliance"in Amsterdam, the Netherlands on 11th-12th November 2004. Leaders from industry, government, standards user organizations and standards developing organizations will attend the conference. CAS (China Association for Standardization) is going to attend the conference and have an address as an invited speaker.

  8. Burstein Moss effect in nanocrystalline CaS : Ce

    Indian Academy of Sciences (India)

    Geeta Sharma; Puja Chawla; S P Lochab; Nafa Singh

    2011-07-01

    The nanocrystalline CaS : Ce nanophosphors are synthesized by wet chemical co-precipitation method. The particles possess an average size of 10 nm as calculated using Debye–Scherrer formula. The particle size and the crystalline nature of the formed nanoparticles are confirmed by TEM micrograph. The optical studies are carried out using UV–Vis absorption spectroscopy. The absorption edge is found to show blue shift with increasing cerium concentration. The shift may be attributed to Burstein Moss effect.

  9. Mass Loss History of the AGB star, R Cas

    CERN Document Server

    Ueta, Toshiya; Yamamura, Issei; Izumiura, Hideyuki; Nakada, Yoshikazu; Matsuura, Mikako; Ita, Yoshifusa; Tanabe, Toshihiko; Fukushi, Hinako; Matsunaga, Noriyuki; Mito, Hiroyuki; Speck, Angela K

    2009-01-01

    We report here on the discovery of an extended far-infrared shell around the AGB star, R Cassiopeia, made by AKARI and Spitzer. The extended, cold circumstellar shell of R Cas spans nearly 3 arcmin and is probably shaped by interaction with the interstellar medium. This report is one of several studies of well-resolved mass loss histories of AGB stars under AKARI and Spitzer observing programs labeled "Excavating Mass Loss History in Extended Dust Shells of Evolved Stars (MLHES)".

  10. CAS projects winning National Natural Science Awards in 2007

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    @@ At the National Conference for Science and Technology Awards held in early 2008 in Beijing,CAS scientists were honored with 13 second-prizes of the National Natural Science Awards,three prizes of the Awards for Technical Invention and 14 second-prizes of National Awards for S&T Progress respectively.The following is a brief introduction to the winner projects of the National Natural Science Awards.

  11. CAS-TWAS-WMO Forum: 12 Years On

    Institute of Scientific and Technical Information of China (English)

    2012-01-01

    In 2000, with the joint efforts of the Chinese Academy of Sciences (CAS), the Academy of Science for the Developing World (TWAS) and the World Meteorological Organization (WMO), a regular international forum (known as CTWF) for physico- mathematical problems related to climate modeling and prediction was founded. Twelve years later, BCAS interviews Prof. ZHU Jiang, deputy director of the CAS Institute of Atmospheric Physics (IAP/CAS), about the development of the Forum.

  12. How specific is CRISPR/Cas9 really?

    OpenAIRE

    O'Geen, H; Yu, AS; Segal, DJ

    2015-01-01

    © 2015 The Authors. The specificity of RNA-guided nucleases has gathered considerable interest as they become broadly applied to basic research and therapeutic development. Reports of the simple generation of animal models and genome engineering of cells raised questions about targeting precision. Conflicting early reports led the field to believe that CRISPR/Cas9 system was promiscuous, leading to a variety of strategies for improving specificity and increasingly sensitive methods to detect ...

  13. Six new DR52-associated DRB1 alleles, three of DR8, two of DR11, and one of DR6, reflect a variety of mechanisms which generate polymorphism in the MHC.

    Science.gov (United States)

    Smith, A G; Nelson, J L; Regen, L; Guthrie, L A; Donadi, E; Mickelson, E M; Hansen, J A

    1996-08-01

    We have sequenced DNA from six new DR52-associated DRB1 alleles initially detected by PCR/SSOP analysis. Three DR8 associated alleles differed from previously known alleles by single nucleotide substitutions. DRB1*0807 and DRB1*0811 both vary from DRB1*08021 at codon 57 resulting in two different amino acids at this residue. DRB1*0807 was identified in samples of Brazilian origin while *0811 was identified among samples from the Tlingit Native American population of Southeast Alaska. DRB1*0814, identified in a family of Chinese origin, differed from DRB1*08032 at codon 12 at both the nucleotide and the amino acid level. In addition, two alleles of DR11, DRB1*1113 and *1119, were each detected in Caucasian individuals. DRB1*1113 differs from other DR11 alleles at codons 37, 67, 70 and 74, while DRB1*1119 differs from *1101 by a single nucleotide substitution at codon 67. Finally, DRB1*1418 was detected in a sample from an Asian or Pacific Islander and shares sequences with several other DR52-associated DRB1 alleles. These six DRB1 alleles appear to have been generated by either gene conversion events, DRB1*1113 and *1418, or by point mutations, DRB1*0814, *0807, *0811 and *1119, although the single nucleotide substitutions found in the latter three alleles are also present in at least one other DRB1 allele and, therefore, could have been the product of gene conversions.

  14. Dr. Anil Kakodkar, Director, Bhabha Atomic Research Centre (BARC), Chairman-Designate of the Atomic Energy Commission Secretary, Department of Atomic Energy, India

    CERN Multimedia

    Patrice Loïez

    2000-01-01

    Photo 01: Dr. A. Kakodkar signing the Guest Book with Dr. L. Evans, LHC Project Leader and Prof. R. Cashmore, Director for Collider Programmes Photo 02: Dr. A. Kakodkar with Dr. L. Evans Photo 03: Dr. A. Kakodkar

  15. CRISPR/Cas-mediated targeted mutagenesis in Daphnia magna.

    Directory of Open Access Journals (Sweden)

    Takashi Nakanishi

    Full Text Available The water flea Daphnia magna has been used as an animal model in ecology, evolution, and environmental sciences. Thanks to the recent progress in Daphnia genomics, genetic information such as the draft genome sequence and expressed sequence tags (ESTs is now available. To investigate the relationship between phenotypes and the available genetic information about Daphnia, some gene manipulation methods have been developed. However, a technique to induce targeted mutagenesis into Daphnia genome remains elusive. To overcome this problem, we focused on an emerging genome editing technique mediated by the clustered regularly interspaced short palindromic repeats/CRISPR-associated (CRISPR/Cas system to introduce genomic mutations. In this study, we targeted a functionally conserved regulator of eye development, the eyeless gene in D. magna. When we injected Cas9 mRNAs and eyeless-targeting guide RNAs into eggs, 18-47% of the survived juveniles exhibited abnormal eye morphology. After maturation, up to 8.2% of the adults produced progenies with deformed eyes, which carried mutations in the eyeless loci. These results showed that CRISPR/Cas system could introduce heritable mutations into the endogenous eyeless gene in D. magna. This is the first report of a targeted gene knockout technique in Daphnia and will be useful in uncovering Daphnia gene functions.

  16. CRISPR/Cas-mediated targeted mutagenesis in Daphnia magna.

    Science.gov (United States)

    Nakanishi, Takashi; Kato, Yasuhiko; Matsuura, Tomoaki; Watanabe, Hajime

    2014-01-01

    The water flea Daphnia magna has been used as an animal model in ecology, evolution, and environmental sciences. Thanks to the recent progress in Daphnia genomics, genetic information such as the draft genome sequence and expressed sequence tags (ESTs) is now available. To investigate the relationship between phenotypes and the available genetic information about Daphnia, some gene manipulation methods have been developed. However, a technique to induce targeted mutagenesis into Daphnia genome remains elusive. To overcome this problem, we focused on an emerging genome editing technique mediated by the clustered regularly interspaced short palindromic repeats/CRISPR-associated (CRISPR/Cas) system to introduce genomic mutations. In this study, we targeted a functionally conserved regulator of eye development, the eyeless gene in D. magna. When we injected Cas9 mRNAs and eyeless-targeting guide RNAs into eggs, 18-47% of the survived juveniles exhibited abnormal eye morphology. After maturation, up to 8.2% of the adults produced progenies with deformed eyes, which carried mutations in the eyeless loci. These results showed that CRISPR/Cas system could introduce heritable mutations into the endogenous eyeless gene in D. magna. This is the first report of a targeted gene knockout technique in Daphnia and will be useful in uncovering Daphnia gene functions.

  17. CRISPR-Cas systems for editing, regulating and targeting genomes.

    Science.gov (United States)

    Sander, Jeffry D; Joung, J Keith

    2014-04-01

    Targeted genome editing using engineered nucleases has rapidly gone from being a niche technology to a mainstream method used by many biological researchers. This widespread adoption has been largely fueled by the emergence of the clustered, regularly interspaced, short palindromic repeat (CRISPR) technology, an important new approach for generating RNA-guided nucleases, such as Cas9, with customizable specificities. Genome editing mediated by these nucleases has been used to rapidly, easily and efficiently modify endogenous genes in a wide variety of biomedically important cell types and in organisms that have traditionally been challenging to manipulate genetically. Furthermore, a modified version of the CRISPR-Cas9 system has been developed to recruit heterologous domains that can regulate endogenous gene expression or label specific genomic loci in living cells. Although the genome-wide specificities of CRISPR-Cas9 systems remain to be fully defined, the power of these systems to perform targeted, highly efficient alterations of genome sequence and gene expression will undoubtedly transform biological research and spur the development of novel molecular therapeutics for human disease.

  18. Targeted mutagenesis using CRISPR/Cas system in medaka

    Directory of Open Access Journals (Sweden)

    Satoshi Ansai

    2014-04-01

    Full Text Available Clustered regularly interspaced short palindromic repeats (CRISPR/CRISPR-associated (Cas system-based RNA-guided endonuclease (RGEN has recently emerged as a simple and efficient tool for targeted genome editing. In this study, we showed successful targeted mutagenesis using RGENs in medaka, Oryzias latipes. Somatic and heritable mutations were induced with high efficiency at the targeted genomic sequence on the DJ-1 gene in embryos that had been injected with the single guide RNA (sgRNA transcribed by a T7 promoter and capped RNA encoding a Cas9 nuclease. The sgRNAs that were designed for the target genomic sequences without the 5′ end of GG required by the T7 promoter induced the targeted mutations. This suggests that the RGEN can target any sequence adjacent to an NGG protospacer adjacent motif (PAM sequence, which occurs once every 8 bp. The off-target alterations at 2 genomic loci harboring double mismatches in the 18-bp targeting sequences were induced in the RGEN-injected embryos. However, we also found that the off-target effects could be reduced by lower dosages of sgRNA. Taken together, our results suggest that CRISPR/Cas-mediated RGENs may be an efficient and flexible tool for genome editing in medaka.

  19. The triple-mode pulsating variable V823 Cas

    CERN Document Server

    Jurcsik, J; Varadi, M; Henden, A; Hurta, Z; Lakatos, B; Posztobanyi, K; Klagyivik, P; Sodor, A; Hurta, Zs.

    2005-01-01

    Based on extended multicolour CCD photometry of the triple-mode radial pulsator V823 Cas we studied the properties of the coupling frequencies invoked by nonlinear processes. Our results support that a resonance connection as suggested by Antonello & Aikawa (1998) affects the mode coupling behaviour. The P1/P0 period ratio of V823 Cas has an "out of range" value if compared with the period ratios of the known double mode pulsators, while the P2/P1 period ratio is normal. The periods and period ratios cannot be consistently interpret without conflict with pulsation and/or evolution models. We attempt to interpret this failure by the suggestion that at present, the periods of V823 Cas are in a transient, resonance affected state, thus do not reflect the true parameters of the object. The anomalous period change behaviour of the fundamental and second overtone modes supports this idea. We have also raised the possibility that a f0 + f2 = 2f1 resonance may act in triple mode pulsators.

  20. Genome engineering using the CRISPR-Cas9 system.

    Science.gov (United States)

    Ran, F Ann; Hsu, Patrick D; Wright, Jason; Agarwala, Vineeta; Scott, David A; Zhang, Feng

    2013-11-01

    Targeted nucleases are powerful tools for mediating genome alteration with high precision. The RNA-guided Cas9 nuclease from the microbial clustered regularly interspaced short palindromic repeats (CRISPR) adaptive immune system can be used to facilitate efficient genome engineering in eukaryotic cells by simply specifying a 20-nt targeting sequence within its guide RNA. Here we describe a set of tools for Cas9-mediated genome editing via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, as well as generation of modified cell lines for downstream functional studies. To minimize off-target cleavage, we further describe a double-nicking strategy using the Cas9 nickase mutant with paired guide RNAs. This protocol provides experimentally derived guidelines for the selection of target sites, evaluation of cleavage efficiency and analysis of off-target activity. Beginning with target design, gene modifications can be achieved within as little as 1-2 weeks, and modified clonal cell lines can be derived within 2-3 weeks.