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Sample records for ps seed particles

  1. Chemical resistance of core-shell particles (PS/PMMA polymerized by seeded suspension

    Directory of Open Access Journals (Sweden)

    Luiz Fernando Belchior Ribeiro

    2017-09-01

    Full Text Available Abstract Core-shell particles were produced on seeded suspension polymerization by using polystyrene (PS as polymer core, or seed, and methyl methacrylate (MMA as the shell forming monomer. Two synthesis routes were evaluated by varying the PS seed conversion before MMA addition. The main purpose of this work was to investigate the influence of synthesis routes on the morphology and chemical resistance of the resulting particles. 1H NMR spectroscopy showed that the use of PS seeds with lower conversion led to the formation of higher amount of poly(styrene-co-MMA. The copolymer acted as a compatibilizer, decreasing the interfacial energy between both homopolymers. As a consequence, a larger amount of reduced PMMA cluster were formed, as was revealed by TEM measurements. Samples in this system showed enhanced resistance to cyclohexane attack compared with pure PS, with a PS extraction of only 37% after 54 hours test.

  2. Hybrid fs/ps CARS for Sooting and Particle-laden Flames [PowerPoint

    Energy Technology Data Exchange (ETDEWEB)

    Hoffmeister, Kathryn N. Gabet; Guildenbecher, Daniel Robert; Kearney, Sean P.

    2016-01-01

    We report the application of ultrafast rotational coherent anti-Stokes Raman scattering (CARS) for temperature and relative oxygen concentration measurements in the plume emanating from a burning aluminized ammonium perchlorate propellant strand. Combustion of these metal-based propellants is a particularly hostile environment for laserbased diagnostics, with intense background luminosity, scattering and beam obstruction from hot metal particles that can be as large as several hundred microns in diameter. CARS spectra that were previously obtained using nanosecond pulsed lasers in an aluminumparticle- seeded flame are examined and are determined to be severely impacted by nonresonant background, presumably as a result of the plasma formed by particulateenhanced laser-induced breakdown. Introduction of fs/ps laser pulses enables CARS detection at reduced pulse energies, decreasing the likelihood of breakdown, while simultaneously providing time-gated elimination of any nonresonant background interference. Temperature probability densities and temperature/oxygen correlations were constructed from ensembles of several thousand single-laser-shot measurements from the fs/ps rotational CARS measurement volume positioned within 3 mm or less of the burning propellant surface. Preliminary results in canonical flames are presented using a hybrid fs/ps vibrational CARS system to demonstrate our progress towards acquiring vibrational CARS measurements for more accurate temperatures in the very high temperature propellant burns.

  3. Hybrid fs/ps CARS for Sooting and Particle-laden Flames

    Energy Technology Data Exchange (ETDEWEB)

    Hoffmeister, Kathryn N. Gabet; Guildenbecher, Daniel Robert; Kearney, Sean P.

    2015-12-01

    We report the application of ultrafast rotational coherent anti-Stokes Raman scattering (CARS) for temperature and relative oxygen concentration measurements in the plume emanating from a burning aluminized ammonium perchlorate propellant strand. Combustion of these metal-based propellants is a particularly hostile environment for laserbased diagnostics, with intense background luminosity, scattering and beam obstruction from hot metal particles that can be as large as several hundred microns in diameter. CARS spectra that were previously obtained using nanosecond pulsed lasers in an aluminumparticle- seeded flame are examined and are determined to be severely impacted by nonresonant background, presumably as a result of the plasma formed by particulateenhanced laser-induced breakdown. Introduction of fs/ps laser pulses enables CARS detection at reduced pulse energies, decreasing the likelihood of breakdown, while simultaneously providing time-gated elimination of any nonresonant background interference. Temperature probability densities and temperature/oxygen correlations were constructed from ensembles of several thousand single-laser-shot measurements from the fs/ps rotational CARS measurement volume positioned within 3 mm or less of the burning propellant surface. Preliminary results in canonical flames are presented using a hybrid fs/ps vibrational CARS system to demonstrate our progress towards acquiring vibrational CARS measurements for more accurate temperatures in the very high temperature propellant burns.

  4. Particle Size Effects on Flow Properties of PS304 Plasma Spray Feedstock Powder Blend

    Science.gov (United States)

    Stanford, Malcolm K.; DellaCorte, Christopher; Eylon, Daniel

    2002-01-01

    The effects of BaF2-CaF2 particle size and size distribution on PS304 feedstock powder flowability have been investigated. Angular BaF2-CaF2 eutectic powders were produced by comminution and classified by screening to obtain 38 to 45 microns 45 to 106 microns, 63 to 106 microns, 45 to 53 microns, 63 to 75 microns, and 90 to 106 microns particle size distributions. The fluorides were added incrementally from 0 to 10 wt% to the other powder constituents of the PS304 feedstock: nichrome, chromia, and silver powders. The flow rate of the powder blends decreased linearly with increasing concentration of the fluorides. Flow was degraded with decreasing BaF2-CaF2 particle size and with increasing BaF2-CaF2 particle size distribution. A semiempirical relationship is offered to describe the PS304 powder blend flow behavior. The Hausner Ratio confirmed the funnel flow test results, but was slightly less sensitive to differences in BaF2-CaF2 particle size and size distribution. These findings may have applicability to other powders that do not flow easily, such as ceramic powders.

  5. Structure of PS/PMMA Blends with Interfacially Active Janus Particles Derived from ABC Triblock Copolymers

    Science.gov (United States)

    Bryson, Kyle; Löbling, Tina; Müller, Axel; Hayward, Ryan; Russell, Thomas

    2014-03-01

    Kinetic trapping of bicontinuous polymer morphologies on submicron length scales through the interfacial adsorption of nanoparticles is of interest due to the unique combination of the properties of each component provided by such structures, and their potential for use as membranes and composite materials. However, this strategy is challenging to realize in polymeric systems, due to the difficulties in preparing particles that are neutrally wetted by the two polymer phases. Janus particles afford a route to circumvent the necessity of neutral wettability. Both theory and experiment have shown enhanced interfacial adsorption energies for Janus particles, as well as greater flexibility in controlling particle orientation at the interface, in comparison to homogeneous particles. Janus particles with polystyrene and poly(methyl methacrylate) (PS/PMMA) hemispheres and a crosslinked polybutadiene core were prepared from triblock copolymers. Using blends of PS and PMMA homopolymers and the Janus particles, we examined structures produced by phase separation during solvent casting and thermodynamic demixing transitions via TEM and small-angle light scattering. The results elucidate the role of particle wettability on interfacial behavior and the structure of stabilized emulsions.

  6. MoS2 PARTICLES MODIFIED WITH POLYSTYRENE FOR PRODUCING Ni–PS/MoS2 COATINGS

    OpenAIRE

    ZHONGJIA HUANG; DANGSHENG XIONG

    2008-01-01

    The MoS2 particles were coated with polystyrene and can be written as PS/MoS2 hereinafter. Ni–PS/MoS2 coatings and Ni–MoS2 coatings were produced by PC electrodeposition technique. The surface morphology of Ni–PS/MoS2 coating was examined and compared with those of Ni–MoS2 coating. The effect of particle concentrations on the volume percent of particles incorporated in the coatings was investigated. And the microhardness of coatings was also investigated. Results show that the surface morphol...

  7. PP and PS joint inversion with a posterior constraint and with particle filtering

    Science.gov (United States)

    Tang, Jing; Wang, Yanfei

    2017-12-01

    The Bayesian framework works well in amplitude versus offset (AVO) inversion, which merges multi-information together to generate posterior distributions of P-wave velocity, S-wave velocity and density. Most existing AVO inversion methods utilize PP reflection seismic data to predict the three elastic parameters. These methods are not usually sensitive to S-wave velocity and density, which make the inversion methods inaccurate and unstable. One way of solving these problems is to perform PP and PS joint inversion by incorporating PS seismic data. Another way is to provide a relatively accurate prior model. In this paper, we apply a particle filtering technique to produce a prior model for the PP and PS joint inversion. In the Bayesian inversion setting, the prior model works as the regularization term. Particle filtering is a Bayesian recursive method that combines prior information with observed data to provide a posterior constraint to reduce the joint inversion’s uncertainty. We generate synthetic models with different signal-to-noise ratios to validate our new method. Comparisons are provided with the traditional joint inversion, which adopts the Gaussian prior model. The inversion results show that the three elastic parameters are retrieved well when the signal-to-noise ratios are high. As the signal-to-noise ratio reduces, our new method can depict more detailed changes than the traditional inversion method, and improves the inversion accuracy apparent in the target layers.

  8. Particle Morphology Effects on Flow Characteristics of PS304 Plasma Spray Coating Feedstock Powder Blend

    Science.gov (United States)

    Stanford, Malcolm K.; DellaCorte, Christopher; Eylon, Daniel

    2002-01-01

    The effects of BaF2-CaF 2 particle morphology on PS304 feedstock powder flow ability have been investigated. BaF2-CaF2 eutectic powders were fabricated by comminution (angular) and by gas atomization (spherical). The fluoride powders were added incrementally to the other powder constituents of the PS304 feedstock: nichrome, chromia, and silver powders. A linear relationship between flow time and concentration of BaF2-CaF2 powder was found. Flow of the powder blend with spherical BaF2-CaF2 was better than the angular BaF2-CaF2. Flow ability of the powder blend with angular fluorides decreased linearly with increasing fluoride concentration. Flow of the powder blend with spherical fluorides was independent of fluoride concentration. Results suggest that for this material blend, particle morphology plays a significant role in powder blend flow behavior, offering potential methods to improve powder flow ability and enhance the commercial potential. These findings may have applicability to other difficult-to-flow powders such as cohesive ceramics.

  9. Preparation of janus particles with different stabilizers and formation of one-dimensional particle arrays.

    Science.gov (United States)

    Onishi, Shohei; Tokuda, Masayoshi; Suzuki, Toyoko; Minami, Hideto

    2015-01-20

    Janus particles with two hemispheres having different stabilizers, a polystyrene (PS) phase stabilized by poly(acrylic acid) (PAA) (PS(PAA)) and a poly(methyl methacrylate) (PMMA) phase stabilized by poly(vinylpyrrolidone) (PVP) (PMMA(PVP)), were synthesized by the solvent-absorbing/releasing method of PS(PAA)/PMMA(PVP) composite particles with a core-shell structure. The PS(PAA)/PMMA(PVP) composite particles were prepared by seeded dispersion polymerization of MMA using PVP as stabilizer in the presence of PS seed particles stabilized by PAA. We also demonstrated the facile formation of the colloidal chains via hydrogen bonding interaction between different stabilizers.

  10. Particle size distributions of several commonly used seeding aerosols

    Science.gov (United States)

    Crosswy, F. L.

    1985-01-01

    During the course of experimentation, no solid particle powder could be found which produced an aerosol with a narrow particle size distribution when fluidization was the only flow process used in producing the aerosol. The complication of adding particle size fractionation processes to the aerosol generation effort appears to be avoidable. In this regard, a simple sonic orifice is found to be effective in reducing the percentage of agglomerates in the several metal oxide powders tested. Marginally beneficial results are obtained for a 0.5/99.5 percent by weight mixture of the flow agent and metal oxide powder. However, agglomeration is observed to be enhanced when the flow agent percentage is increased to 5 percent. Liquid atomization using the Collison nebulizer as well as a version of the Laskin nozzle resulted in polydispersed aerosols with particle size distributions heavily weighted by the small particle end of the size spectrum. The aerosol particle size distributions produced by the vaporization/condensation seeder are closer to the ideal monodispersed aerosol than any of the other aerosols tested. In addition, this seeding approach affords a measure of control over particle size and particle production rate.

  11. Mission Prospects: Remote Sensing of Coronal Suprathermal Seed Particles

    Science.gov (United States)

    Moses, J.; Laming, J. M.; Ko, Y.

    2013-12-01

    A new perspective on the feasibility of remote sensing detection and characterization of suprathermal particles serving the role of seeds in solar energetic particle (SEP) acceleration has recently been achieved via a combination of theoretical (Laming et al. 2013 http://dx.doi.org/10.1088/0004-637X/770/1/73) and experimental (Kohl et al. 2011 http://arxiv.org/abs/1104.3817, Moses et al. 2011 http://dx.doi.org/10.1117/12.896868) developments. The location, density and velocity distribution of these seed particles can now be established as science observing requirements for instrument specification. The most promising instrumentation for these observations is a UV coronal spectrometer. While the spectroscopic and spatial resolution of the one existing, spaceflight UV coronal spectrometer - SOHO UVCS - meets this specification; the effective area of this instrument is two orders of magnitude too low. Initial attempts at achieving increased effective area by directly scaling UVCS have not been successfully transitioned into flight programs (e.g. Gardner et al. 1999, 'Advanced Solar Coronal Explorer Mission (ASCE)', SPIE 3764, 134). With the recent development of instrument designs achieving the requisite increase in effective area within a volume envelope comparable to UVCS, the options for achieving a successful flight program have been increased. This work is supported in part by the Office of Naval Research/Naval Research Laboratory Basic Research (6.1) Program and NASA-DoD PR 12SHP12/2-0151.

  12. Bio-Functional, Lanthanide-Labeled Polymer Particles by Seeded Emulsion Polymerization and their Characterization by Novel ICP-MS Detection.

    Science.gov (United States)

    Thickett, Stuart C; Abdelrahman, Ahmed I; Ornatsky, Olga; Bandura, Dmitry; Baranov, Vladimir; Winnik, Mitchell A

    2010-01-01

    We present the synthesis and characterization of monodisperse, sub-micron poly(styrene) (PS) particles loaded with up to and including 10(7) lanthanide (Ln) ions per particle. These particles have been synthesized by seeded emulsion polymerization with a mixture of monomer and a pre-formed Ln complex, and analyzed on a particle-by-particle basis by a unique inductively coupled plasma mass cytometer. Seed particles were prepared by surfactant-free emulsion polymerization (SFEP) to obtain large particle sizes in aqueous media. Extensive surface acid functionality was introduced using the acid-functional initiator ACVA, either during seed latex synthesis or in the second stage of polymerization. The loading of particles with three different Ln ions (Eu, Tb, and Ho) has proven to be close to 100 % efficient on an individual and combined basis. Covalent attachment of metal-tagged peptides and proteins such as Neutravidin to the particle surface was shown to be successful and the number of bound species can be readily determined. We believe these particles can serve as precursors for multiplexed, bead-based bio-assays utilizing mass cytometric detection.

  13. Expression of PsGRP1, a novel glycine rich protein gene of Pisum sativum, is induced in developing fruit and seed and by ABA in pistil and root.

    Science.gov (United States)

    Urbez, Cristina; Cercós, Manuel; Perez-Amador, Miguel A; Carbonell, Juan

    2006-05-01

    A novel glycine-rich protein gene, PsGRP1, has been identified in Pisum sativum L. Accumulation of PsGRP1 transcripts was observed in reproductive organs and vegetative tissues. They were localized in endocarp sclerenchyma during fruit development in cells that will lignify. PsGRP1 expression was also detected in senescent pistils and developing seeds and induced by ABA treatment in presenescent pistils. A raise in the expression was also observed in roots after treatment with ABA or mannitol but not under cold stress. A mannitol treatment induced a rise in ABA levels and fluridone treatment counteracted the mannitol induction of PsGRP1 expression. The results suggest a possible role for PsGRP1 in differentiation of the endocarp sclerenchyma and during seed development, pistil senescence and osmotic stress under ABA control.

  14. Characteristics of dust particles abraded from pesticide treated seeds: 1. Size distribution using different measuring techniques.

    Science.gov (United States)

    Foqué, Dieter; Zwertvaegher, Ingrid Ka; Devarrewaere, Wouter; Verboven, Pieter; Nuyttens, David

    2017-07-01

    Particle size is one of the most important properties affecting the driftability and behaviour of dust particles abraded from pesticide dressed seeds during sowing. Three particle sizing techniques were used determine the particle size distribution of dust abraded from seeds from six different species. Important differences in dust particle size distribution between species were observed with the finest dust for rapeseed and the coarsest dust for barley. Wet laser diffraction and sonic sieving particle size results correlated well while micro-CT is able to deliver three-dimensional information and additional physical particle properties (shape, porosity). All particle sizing techniques have their (dis)advantages and none of them is able to perfectly describe the real size distribution of non-spherical particles. The particle size information gathered can be used in dust drift prediction models, risk assessment tools and will help to better understand the dust drift phenomenon. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

  15. Investigating the use of secondary organic aerosol as seed particles in simulation chamber experiments

    Directory of Open Access Journals (Sweden)

    J. F. Hamilton

    2011-06-01

    Full Text Available The use of β-caryophyllene secondary organic aerosol particles as seeds for smog chamber simulations has been investigated. A series of experiments were carried out in the Manchester photochemical chamber as part of the Aerosol Coupling in the Earth System (ACES project to study the effect of seed particles on the formation of secondary organic aerosol (SOA from limonene photo-oxidation. Rather than use a conventional seed aerosol containing ammonium sulfate or diesel particles, a method was developed to use in-situ chamber generated seed particles from β-caryophyllene photo-oxidation, which were then diluted to a desired mass loading (in this case 4–13 μg m−3. Limonene was then introduced into the chamber and oxidised, with the formation of SOA seen as a growth in the size of oxidised organic seed particles from 150 to 325 nm mean diameter. The effect of the partitioning of limonene oxidation products onto the seed aerosol was assessed using aerosol mass spectrometry during the experiment and the percentage of m/z 44, an indicator of degree of oxidation, increased from around 5 to 8 %. The hygroscopicity of the aerosol also changed, with the growth factor for 200 nm particles increasing from less than 1.05 to 1.25 at 90 % RH. The detailed chemical composition of the limonene SOA could be extracted from the complex β-caryophyllene matrix using two-dimensional gas chromatography (GC × GC and liquid chromatography coupled to mass spectrometry. High resolution Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FTICR-MS was used to determine exact molecular formulae of the seed and the limonene modified aerosol. The average O:C ratio was seen to increase from 0.32 to 0.37 after limonene oxidation products had condensed onto the organic seed.

  16. Distinct element analysis of inter-particle coating variability in a batch seed coater

    Science.gov (United States)

    Pasha, Mehrdad; Ghadiri, Mojtaba; Gunadi, Alfeno; Piccione, Patrick M.

    2017-06-01

    Coating of particulate solids by a thin film layer is of interest in many industrial applications such as seed and tablet coating. In seed processing, seeds are commonly coated with a protective coating layer consisting of fertilisers and crop protection products. Rotary drum batch coaters are typically used for this purpose. The coater consists of a cylindrical vessel with a rotating spray disk in the centre, onto which the coating liquid is fed. The seeds are driven around the vessel by its rotating base, and are mixed by two baffles; one on either side of the vessel. In the present study, DEM simulations are used to analyse the seed coating process. Corn seed is used as a model material and its shape is captured using X-Ray micro-tomography (XRT). The shape is incorporated into the simulations by the clumping multiple spheres to form a particle assembly. The coating uniformity of the seeds is predicted by implementing a coating model, whereby the coating mechanism is represented in the DEM by considering that once a droplet contacts a corn seed, it is removed from the simulation and its mass is attributed to the coating mass of the corn seed. The distribution of mass of sprayed spheres on the corn seeds and the coefficient of variation are evaluated for a range of process conditions, such as spinning disk rotational speed, droplets size and baffle arrangement and designs. In addition to evaluation of coating uniformity of particles, the scale-up rules for rotary drum batch coaters are investigated and reported. The outcome provides guidelines on scale-up rules and improvement of coating uniformity for rotary batch seed coaters.

  17. Silica core/conjugated polymer shell particles via seeded Knoevenagel dispersion polymerization - laser action in whispering gallery mode resonators.

    Science.gov (United States)

    Ciftci, Sibel; Mikosch, Annabel; Haehnle, Bastian; Witczak, Łukasz; Kuehne, Alexander J C

    2016-12-06

    Here, we present a seeded Knoevenagel dispersion polymerization to generate hybrid particles with a conjugated polymer shell on inorganic silica cores. This seeded dispersion polymerization facilitates the generation of core-shell particles, which exhibit whispering gallery mode lasing. The lasing threshold decreases while the spectral range of emission increases with increasing shell thickness. This novel seeded Knoevenagel dispersion polymerization opens up a facile and metal free pathway towards single particle conjugated polymer lasers on the micrometer scale.

  18. Vortex Formation in a Shock-Accelerated Gas Induced by Particle Seeding

    Science.gov (United States)

    Vorobieff, Peter; Anderson, Michael; Conroy, Joseph; White, Ross; Truman, C. Randall; Kumar, Sanjay

    2011-05-01

    An instability forms in gas of constant density (air) with an initial nonuniform seeding of small particles or droplets as a planar shock wave passes through the two-phase medium. The seeding nonuniformity is produced by vertical injection of a slow-moving jet of air premixed with glycol droplets or smoke particles into the test section of a shock tube, with the plane of the shock parallel to the axis of the jet. After the shock passage, two counterrotating vortices form in the plane normal to that axis. The physical mechanism of the instability we observe is peculiar to multiphase flow, where the shock acceleration causes the second (embedded) phase to move with respect to the embedding medium. With sufficient seeding concentration, this leads to entrainment of the embedding phase that acquires a relative velocity dependent on the initial seeding, resulting in vortex formation in the flow.

  19. Cu particle seeded InP-InAs axial nanowire heterostructures

    Energy Technology Data Exchange (ETDEWEB)

    Hillerich, Karla; Ghidini, Dario S.; Deppert, Knut; Johansson, Jonas [Solid State Physics, Lund University, Lund (Sweden); Dick, Kimberly A. [Solid State Physics, Lund University, Lund (Sweden); Polymer and Materials Chemistry, Lund University, Lund (Sweden)

    2013-10-15

    We demonstrate the epitaxial growth of alternating InP-InAs nanowire heterostructures using Cu seed particles in MOVPE. We observe extraordinary early stages in the formation of InAs segments, e.g. three-dimensional nucleation instead of step-flow growth. Furthermore, InAs segments of thin nanowires exhibit extended 4H crystal structure. Color coded XEDS elemental map of a typical InP-InAs nanowire heterostructure seeded by a Cu particle. (copyright 2013 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim) (orig.)

  20. Finding the Force -- Consistent Particle Seeding for Satellite Aerodynamics

    CERN Document Server

    Parham, J Brent

    2013-01-01

    When calculating satellite trajectories in low-earth orbit, engineers need to adequately estimate aerodynamic forces. But to this day, obtaining the drag acting on the complicated shapes of modern spacecraft suffers from many sources of error. While part of the problem is the uncertain density in the upper atmosphere, this works focuses on improving the modeling of interacting rarified gases and satellite surfaces. The only numerical approach that currently captures effects in this flow regime---like self-shadowing and multiple molecular reflections---is known as test-particle Monte Carlo. This method executes a ray-tracing algorithm to follow particles that pass through a control volume containing the spacecraft and accumulates the momentum transfer to the body surfaces. Statistical fluctuations inherent in the approach demand particle numbers in the order of millions, often making this scheme too costly to be practical. This work presents a parallel test-particle Monte Carlo method that takes advantage of b...

  1. PIV for the characterization of focused field induced acoustic streaming: seeding particle choice evaluation.

    Science.gov (United States)

    Ben Haj Slama, Rafika; Gilles, Bruno; Ben Chiekh, Maher; Béra, Jean-Christophe

    2017-04-01

    This research evaluates the use of Particle Image Velocimetry (PIV) technique for characterizing acoustic streaming flow generated by High Intensity Focused Ultrasound (HIFU). PIV qualification tests, focusing on the seeding particle size (diameter of 5, 20 and 50μm) were carried out in degassed water subjected to a focused field of 550kHz-frequency with an acoustic pressure amplitude of 5.2, 10.5 and 15.7bar at the focus. This study shows that the ultrasonic field, especially the radiation force, can strongly affect seeding particle behavior. Large particles (50μm-diameter) are repelled from the focal zone and gathered at radiation pressure convergence lines on either side of the focus. The calculation of the acoustic radiation pressure applied on these particles explains the observed phenomenon. PIV measurements do not, therefore, properly characterize the streaming flow in this case. On the contrary, small particles (5μm-diameter) velocity measurements were in good agreement with the Computational Fluid Dynamics (CFD) simulations of the water velocity field. A simple criterion approximating the diameter threshold below which seeding particles are qualified for PIV in presence of focused ultrasound is then proposed. Copyright © 2017 Elsevier B.V. All rights reserved.

  2. Quantitative 3D shape description of dust particles from treated seeds by means of X-ray micro-CT.

    Science.gov (United States)

    Devarrewaere, Wouter; Foqué, Dieter; Heimbach, Udo; Cantre, Dennis; Nicolai, Bart; Nuyttens, David; Verboven, Pieter

    2015-06-16

    Crop seeds are often treated with pesticides before planting. Pesticide-laden dust particles can be abraded from the seed coating during planting and expelled into the environment, damaging nontarget organisms. Drift of these dust particles depends on their size, shape and density. In this work, we used X-ray micro-CT to examine the size, shape (sphericity) and porosity of dust particles from treated seeds of various crops. The dust properties quantified in this work were very variable in different crops. This variability may be a result of seed morphology, seed batch, treatment composition, treatment technology, seed cleaning or an interaction of these factors. The intraparticle porosity of seed treatment dust particles varied from 0.02 to 0.51 according to the crop and generally increased with particle size. Calculated settling velocities demonstrated that accounting for particle shape and porosity is important in drift studies. For example, the settling velocity of dust particles with an equivalent diameter of 200 μm may vary between 0.1 and 1.2 m s(-1), depending on their shape and density. Our analysis shows that in a wind velocity of 5 m s(-1), such particles ejected at 1 m height may travel between 4 and 50 m from the source before settling. Although micro-CT is a valuable tool to characterize dust particles, the current image processing methodology limits the number of particles that can be analyzed.

  3. Particle Size Control for PIV Seeding Using Dry Ice

    Science.gov (United States)

    2010-03-01

    in flight actually being carried out, the observations, drawings and notes of Leonardo da Vinci showed an analytical process to develop a way for...theoretical particle response: dvp dt = −C(vp − U) C = 18µ ρpd2p 86 87 Bibliography 1. Linscott, R. N. and Da Vinci , L., The Notebooks of Leonardo Da Vinci

  4. Inclusive particle production in the transverse momentum range between 0. 25 and 40 GeV/c at the CERN Sp anti pS collider

    Energy Technology Data Exchange (ETDEWEB)

    Banner, M.; Bloch, P.; Lancon, E.; Loucatos, S.; Mansoulie, B.; Polverel, M.; Roussarie, A.; Teiger, J.; Zaccone, H.; Bonaudi, F.

    1985-04-01

    Inclusive particle production cross-sections have been measured at the Sp anti pS collider using the UA2 detector in various ranges of transverse momentum (Psub(T)) and pseudo-rapidity (eta). Cross-section measurements are presented for ..pi../sup 0/ production (Psub(T) <= 15 GeV/c, vertical strokeetavertical stroke<=0.85 and Psub(T) <= 40 GeV/c, 1.0<=vertical strokeetavertical stroke<=1.8), for eta meson production (3 <= Psub(T)<=6 GeV/c, vertical strokeetavertical stroke<=0.85) and for charged particle production (Psub(T) <= 10 GeV/c, 1.0<=vertical strokeetavertical stroke1.8). Results are compared with the predictions of QCD calculations.

  5. Measuring of filtration efficiency of nonwoven textiles in volume from scattered light by seeding particles

    Directory of Open Access Journals (Sweden)

    Sidlof P.

    2013-04-01

    Full Text Available This paper deals with the method which calculates a filtration efficiency of nonwoven textiles from scattered light intensity by seeding particles. Thefiltration efficiency is commonly measured by particle counters. Samples of liquid or gas are taken during a test in front of and behind a filtration material. The concentration of particles is measured and the filtration efficiency is calculated. The filtration efficiency does not have to be uniform in itswhole surface. The uniformity of filtration is another indicator of a quality of filtration materials. Measurements described in this article were performed on a water filtration setup which enables optical access to the place where the filtration material is mounted. Pictures of illuminated seeding particles are made by a laser sheet and a camera. Visualisation of the filtration process enables measuring of the efficiency of separation versus time and also versus two-dimensional position in case of use of a traverse mechanism. The filtration textiles were tested by 1 μm seeding particles. Mean value of light intensity and number of bright pixels in evaluative areas during image analysis were obtained. On the basis of these data, the filtration efficiency iscalculated. The best image analysis method was chosen.

  6. Biosynthesis of silver fine particles and particles decorated with nanoparticles using the extract of Illicium verum (star anise) seeds

    Science.gov (United States)

    Luna, Carlos; Chávez, V. H. G.; Barriga-Castro, Enrique Díaz; Núñez, Nuria O.; Mendoza-Reséndez, Raquel

    2015-04-01

    Given the upsurge of new technologies based on nanomaterials, the development of sustainable methods to obtain functional nanostructures has become an imperative task. In this matter, several recent researches have shown that the biodegradable natural antioxidants of several plant extracts can be used simultaneously as reducing and stabilizing agents in the wet chemical synthesis of metallic nanoparticles, opening new opportunities to design greener synthesis. However, the challenge of these new techniques is to produce stable colloidal nanoparticles with controlled particle uniformity, size, shape and aggregation state, in similar manner than the well-established synthetic methods. In the present work, colloidal metallic silver nanoparticles have been synthesized using silver nitrate and extracts of Illicium verum (star anise) seeds at room temperature in a facile one-step procedure. The resulting products were colloidal suspensions of two populations of silver nanoparticles, one of them with particle sizes of few nanometers and the other with particles of tens of nm. Strikingly, the variation of the AgNO3/extract weight ratio in the reaction medium yielded to the variation of the spatial distribution of the nanoparticles: high AgNO3/extract concentration ratios yielded to randomly dispersed particles, whereas for lower AgNO3/extract ratios, the biggest particles appeared coated with the finest nanoparticles. This biosynthesized colloidal system, with controlled particle aggregation states, presents plasmonic and SERS properties with potential applications in molecular sensors and nanophotonic devices.

  7. Demonstration of Clean Particle Seeding for Particle Image Velocimetry in a Closed Circuit Supersonic Wind Tunnel

    Science.gov (United States)

    2007-03-01

    not as common in PIV. Image Capture As of late, digital imagers are used exclusively in PIV, but in earlier times film photography was the norm...site and a set of runs was accomplished. The injector was set with the tip approximately two inches deep into the cavity of the stagnation chamber...particles to the left of where the laser sheet hits for image 2. The movement of light in the illumination plane tricks both the eye of the observer

  8. Effect of oxidant concentration, exposure time, and seed particles on secondary organic aerosol chemical composition and yield

    Directory of Open Access Journals (Sweden)

    A. T. Lambe

    2015-03-01

    This similarity in turn suggests that both in the flow reactor and in chambers, SOA chemical composition at low OH exposure is governed primarily by gas-phase OH oxidation of the precursors rather than heterogeneous oxidation of the condensed particles. In general, SOA yields measured in the flow reactor are lower than measured in chambers for the range of equivalent OH exposures that can be measured in both the flow reactor and chambers. The influence of sulfate seed particles on isoprene SOA yield measurements was examined in the flow reactor. The studies show that seed particles increase the yield of SOA produced in flow reactors by a factor of 3 to 5 and may also account in part for higher SOA yields obtained in the chambers, where seed particles are routinely used.

  9. Effect of particle size and concentration on the mechanical properties of polyester/date palm seed particulate composites

    Directory of Open Access Journals (Sweden)

    Alewo Opuada AMEH

    2015-05-01

    Full Text Available The use of cellulosic materials as reinforcement in composites can greatly enhance their properties. The thrust of this study was to investigate the effect of date palm seed particle on the properties of reinforced polyester. Unsaturated polyester resin was reinforced with date palm seed particles of 0.5, 2.0 and 2.8mm particle sizes using variable particle loadings of 5, 10, 15, 20 and 25wt%. The composites obtained were subjected to various types of mechanical and physical tests in order to assess their performance. The optimum tensile strength of 16.7619N/mm2 and elastic modulus of 343.8N/mm2 were attained at 15wt% and 10wt% loading (using 0.5mm particles respectively and percent water absorption was found to be least for 0.5mm particle size. The hardness was enhanced to the maximum of 74 HRF (Rockwell Hardness Factor by 2mm particle size at 25wt% loading. Pure unsaturated polyester resin recorded tensile strength of 17.5959N/mm2, elastic modulus of 316.7N/mm2 and hardness of 33.5 HRF. The results indicated that the use of date palm seed particles as reinforcement can enhance the properties of polyester composites.

  10. Fabrication of ITO particles using a combination of a homogeneous precipitation method and a seeding technique and their electrical conductivity

    Directory of Open Access Journals (Sweden)

    Yoshio Kobayashi

    2015-09-01

    Full Text Available The present work proposes a method to fabricate indium tin oxide (ITO particles using precursor particles synthesized with a combination of a homogeneous precipitation method and a seeding technique, and it also describes their electronic conductivity properties. Seed nanoparticles were produced using a co-precipitation method with aqueous solutions of indium (III chloride, tin (IV chloride aqueous solution and sodium hydroxide. Three types of ITO nanoparticles were fabricated. The first type was fabricated using the co-precipitation method (c-ITO. The second and third types were fabricated using a homogeneous precipitation method with the seed nanoparticles (s-ITO and without seeds (n-ITO. The as-prepared precursor particles were annealed in air at 500 °C, and their crystal structures were cubic ITO. The c-ITO nanoparticles formed irregular-shaped agglomerates of nanoparticles. The n-ITO nanoparticles had a rectangular-parallelepiped or quasi-cubic structure. Most s-ITO nanoparticles had a quasi-cubic structure, and their size was larger than the n-ITO particles. The volume resistivities of the c-ITO, n-ITO and s-ITO powders decreased in that order because the regular-shaped particles were made to strongly contact with each other.

  11. Preparation of stable tea seed oil nano-particle emulsions by a low energy method with non-ionic surfactants

    Directory of Open Access Journals (Sweden)

    M. Kanlayavattanakul

    2017-06-01

    Full Text Available Tea seed oil nano-particle emulsions were prepared. Non-ionic surfactants containing Tween 80 and Span 80 (1:1, w/w were mixed with propanol (3-9:1, w/w to give Smix, which was thereafter mixed with tea seed oil. The mixture was titrated with water at 150 rpm to give clear or bluish and bluish-white emulsions. Twelve nano-particle emulsions with 64.64 to 72.73% Smix, 16.66 to 27.27% oil and 9.09 to 16.67% water with particle sizes between 207.00 to 430.10 nm, PDI of 0 to 0.4, ζ-potential of -42.00 to -49.63 mV, pH of 7.04 to 7.32 and 151.33 to 241.93 cps, were stable following an accelerated stability test and long term storage at room temperature and 4 and 45 ºC for 90 days, although one system (16.66% oil and 66.67% Smix was separated. This nano-particle emulsion formulation is concise and feasible for an industrial development of topical products containing tea seed oil.

  12. SU-E-T-259: Particle Swarm Optimization in Radial Dose Function Fitting for a Novel Iodine-125 Seed

    Energy Technology Data Exchange (ETDEWEB)

    Wu, X [University of Alabama at Birmingham, Birmingham, Al (United States); Duan, J; Popple, R; Huang, M; Shen, S; Brezovich, I [University of Alabama Birmingham, Birmingham, AL (United States); Cardan, R [UAB University of Alabama, Birmingham, Birmingham, AL (United States); Benhabib, S [University of Alabama at Birmingham, Birmingham, AL (United States)

    2014-06-01

    Purpose: To determine the coefficients of bi- and tri-exponential functions for the best fit of radial dose functions of the new iodine brachytherapy source: Iodine-125 Seed AgX-100. Methods: The particle swarm optimization (PSO) method was used to search for the coefficients of the biand tri-exponential functions that yield the best fit to data published for a few selected radial distances from the source. The coefficients were encoded into particles, and these particles move through the search space by following their local and global best-known positions. In each generation, particles were evaluated through their fitness function and their positions were changed through their velocities. This procedure was repeated until the convergence criterion was met or the maximum generation was reached. All best particles were found in less than 1,500 generations. Results: For the I-125 seed AgX-100 considered as a point source, the maximum deviation from the published data is less than 2.9% for bi-exponential fitting function and 0.2% for tri-exponential fitting function. For its line source, the maximum deviation is less than 1.1% for bi-exponential fitting function and 0.08% for tri-exponential fitting function. Conclusion: PSO is a powerful method in searching coefficients for bi-exponential and tri-exponential fitting functions. The bi- and tri-exponential models of Iodine-125 seed AgX-100 point and line sources obtained with PSO optimization provide accurate analytical forms of the radial dose function. The tri-exponential fitting function is more accurate than the bi-exponential function.

  13. Effect of nanoscale Fe(3)O(4), TiO(2) and carbon particles on cucumber seed germination.

    Science.gov (United States)

    Mushtaq, Yasmeen K

    2011-01-01

    The effects of Fe(3)O(4), TiO(2) and carbon nanoparticles on the cucumber (Cucumis sativus) seed germination rate, root elongation and germination index were studied. The nanoparticles used ranged in average particle size from 30-50 nm, and their concentration ranged from 0 to 5000 μg/mL. Inhibitory effects were observed and the reduction in root growth was more obvious than the seed germination percentages. In all cases some perturbations of the normal functions with respect to control in germinating test were observed. The results obtained provide a better understanding of the phytotoxicity of the nanomaterials used in this study, and may help to further understand the interaction of nanoparticles with the environment prior to their use in agriculture.

  14. Last PS magnet refurbished

    CERN Document Server

    2009-01-01

    PS Magnet Refurbishment Programme Completed. The 51st and final refurbished magnet was transported to the PS on Tuesday 3 February. The repair and consolidation work on the PS started back in 2003 when two magnets and a busbar connection were found to be faulty during routine high-voltage tests. The cause of the fault was a combination of age and radiation on electrical insulation. After further investigation the decision was taken to overhaul half of the PS’s 100 magnets to reduce the risk of a similar fault. As from 20 February the PS ring will start a five-week test programme to be ready for operation at the end of March.

  15. Rapidity and species dependence of particle production at largetransverse momentum for d+Au collisions at psNN = 200 GeV

    Energy Technology Data Exchange (ETDEWEB)

    Abelev, B.I.; Adams, J.; Aggarwal, M.M.; Ahammed, Z.; Amonett,J.; Anderson, B.D.; Anderson, M.; Arkhipkin, D.; Averichev, G.S.; Bai,Y.; Balewski, J.; Barannikova, O.; Barnby, L.S.; Baudot, J.; Bekele, S.; Belaga, V.V.; Bellingeri-Laurikainen, A.; Bellwied, R.; Benedosso, F.; Bhardwaj, S.; Bhasin, A.; Bhati, A.K.; Bichsel, H.; Bielcik, J.; Bielcikova, J.; Bland, L.C.; Blyth, S.-L.; Bonner, B.E.; Botje, M.; Bouchet, J.; Brandin, A.V.; Bravar, A.; Bystersky, M.; Cadman, R.V.; Cai,X.Z.; Caines, H.; Calderon de la Barca Sanchez, M.; Castillo, J.; Catu,O.; Cebra, D.; Chajecki, Z.; Chaloupka, P.; Chattopadhyay, S.; Chen,H.F.; Chen, J.H.; Cheng, J.; Cherney, M.; Chikanian, A.; Christie, W.; Coffin, J.P.; Cormier, T.M.; Cosentino, M.R.; Cramer, J.G.; Crawford,H.J.; Das, D.; Das, S.; Daugherity, M.; de Moura, M.M.; Dedovich, T.G.; DePhillips, M.; Derevschikov, A.A.; Didenko, L.; Dietel, T.; Djawotho,P.; Dogra, S.M.; Dong, W.J.; Dong, X.; Draper, J.E.; Du, F.; Dunin, V.B.; Dunlop, J.C.; Dutta Mazumdar, M.R.; Eckardt, V.; Edwards, W.R.; Efimov,L.G.; Emelianov, V.; Engelage,J.; Eppley, G.; Erazmus, B.; Estienne, M.; Fachini, P.; Fatemi, R.; Fedorisin, J.; Filimonov, K.; Filip, P.; Finch,E.; Fine, V.; Fisyak, Y.; Fu, J.; Gagliardi, C.A.; Gaillard, L.; Ganti,M.S.; Ghazikhanian, V.; Ghosh, P.; Gonzalez, J.S.; Gorbunov, Y.G.; Gos,H.; Grebenyuk, O.; Grosnick, D.; Guertin, S.M.; Guimaraes, K.S.F.F.; Guo,Y.; Gupta, N.; Gutierrez, T.D.; Haag, B.; Hallman, T.J.; Hamed, A.; Harris, J.W.; He, W.; Heinz, M.; Henry, T.W.; Hepplemann, S.; Hippolyte,B.; Hirsch, A.; Hjort, E.; Hoffman, A.M.; Hoffmann, G.W.; Horner, M.J.; Huang, H.Z.; Huang, S.L.; Hughes, E.W.; Humanic, T.J.; Igo, G.; Jacobs,P.; Jacobs, W.W.; Jakl, P.; Jia, F.; Jiang, H.; Jones, P.G.; Judd, E.G.; Kabana, S.; Kang, K.; Kapitan, J.; Kaplan, M.; Keane, D.; Kechechyan, A.; Khodyrev, V.Yu.; Kim, B.C.; Kiryluk, J.; Kisiel, A.; Kislov, E.M.; Klein,S.R.; Kocoloski, A.; Koetke, D.D.; et al.

    2006-12-19

    We determine rapidity asymmetry in the production of charged pions, protons and anti-protons for large transverse momentum (p{sub T}) for d+Au collisions at {radical}s{sub NN} = 200 GeV. The rapidity asymmetry is defined as the ratio of particle yields at backward rapidity (Au beam direction or -ve rapidity) to those at forward rapidity (d beam direction or +ve rapidity). The identified hadrons are measured in the rapidity regions |y| < 0.5 and 0.5 < |y| < 1.0 for the p{sub T} range 2.5 < p{sub T} < 10 GeV/c. We observe significant rapidity asymmetry for charged pion and proton+anti-proton production in both rapidity regions. The asymmetry is larger for 0.5 < |y| < 1.0 than for |y| < 0.5 and is almost independent of particle type. The measurements are compared to various model predictions employing multiple scattering, energy loss, nuclear shadowing, saturation effects, and recombination, and also to a phenomenological parton model. We find that asymmetries are sensitive to model parameters and show model-preference. The rapidity dependence of {pi}{sup -}/{pi}{sup +} and {bar p}/p ratios in peripheral d+Au and forward neutron-tagged events are used to study the contributions of valence quarks and gluons to particle production at high p{sub T}. The results are compared to calculations based on NLO pQCD and other measurements of quark fragmentation functions.

  16. Performing Particle Image Velocimetry in a Supersonic Wind Tunnel Using Carbon Dioxide as the Seed Material

    Science.gov (United States)

    2007-06-01

    taken in a dark environment ( blackened room) 24 with the seeds being illuminated briefly by a planar stroboscopic light source. This technique...Systems). Industrial carbon dioxide is generally stored as a liquefied compressed gas in large metal tanks at approximately 853 psia or 57 atm...Refraction 1.000449 - - 2. 5 CO2 Piping Effects Compressed carbon dioxide stored in metal tanks exists in both liquid and gas phases. The top

  17. Inside the PS tunnel

    CERN Multimedia

    1974-01-01

    Pre-start work is going on at the end of the PS long shut-down. The photo shows secondary beams drawn from an internal target (bottom) towards South Hall, behind the shielding wall (top) (see also photo 7409012X).

  18. PS Control Room

    CERN Multimedia

    CERN PhotoLab

    1963-01-01

    The good old PS Control Room, all manual. For each parameter, a knob or a button to control it; for each, a light or meter or oscilloscope to monitor it; carefully written pages serve as the data bank; phones and intercom for communication. D.Dekkers is at the microphone, M.Valvini sits in front.

  19. PS auxiliary magnet

    CERN Multimedia

    CERN PhotoLab

    1974-01-01

    Units of the PS auxiliary magnet system. The picture shows how the new dipoles, used for vertical and horizontal high-energy beam manipulation, are split for installation and removal so that it is not necessary to break the accelerator vacuum. On the right, adjacent to the sector valve and the windings of the main magnet, is an octupole of the set.

  20. Transfer line TT70 (electrons from PS to SPS)

    CERN Multimedia

    CERN PhotoLab

    1981-01-01

    As injectors for LEP, PS and SPS had to be converted to the acceleration of electrons and positrons. So far, only positively charged particles had been transferred from the PS to the SPS, for the negatively charged electrons a new transfer line, TT70, had to be built. Due to the difference in level of the two machines, the transfer line slopes and tilts.

  1. Micro-seeding and soft template effects on the control of polymorph and morphology of HMX micro particles in solvent-antisolvent process

    OpenAIRE

    Sajjad Damiri; Shahriar Namvar; Hadi Panahi

    2017-01-01

    A seeding strategy was developed in the preparation of cyclotetramethylenetetranitramine (HMX) explosive micro-particles by solvent-antisolvent method, to control their polymorphs from dangerous gamma (γ) type to the desired and standard beta (β) form with the size distribution of

  2. SPECTRAL PROPERTIES OF LARGE GRADUAL SOLAR ENERGETIC PARTICLE EVENTS. I. FE, O, AND SEED MATERIAL

    Energy Technology Data Exchange (ETDEWEB)

    Desai, M. I.; Dayeh, M. A.; Ebert, R. W.; Mccomas, D. J.; Schwadron, N. A. [Southwest Research Institute, 6220 Culebra Road, San Antonio, TX 78238 (United States); Mason, G. M. [Johns Hopkins University/Applied Physics Laboratory, Laurel, MD 20723 (United States); Li, G. [CSPAR, University of Alabama in Huntsville, Huntsville, AL 35756 (United States); Cohen, C. M. S.; Mewaldt, R. A. [California Institute of Technology, Pasadena, CA 91125 (United States); Smith, C. W., E-mail: mdesai@swri.edu [Department of Physics and Space Science Center, University of New Hampshire, Durham, NH 03824 (United States)

    2016-01-10

    We have surveyed ∼0.1–100 MeV nucleon{sup −1} O and Fe fluence spectra during 46 isolated, large gradual SEP events observed at ACE during solar cycles 23 and 24. Most SEP spectra are well represented by the four-parameter Band function with a normalization constant, low-energy spectral slope, high-energy spectral slope, and break energy. The O and Fe spectral slopes are similar and most spectra steepen above the break energy, probably due to common acceleration and transport processes affecting different ion species. SEP spectra above the break energies depend on the origin of the seed population; larger contributions of suprathermal flare material result in higher Fe/O ratios and flatter spectra at higher energies. SEP events with steeper O spectra at low energies and higher break energies are associated with slower coronal mass ejections (CMEs), while those associated with fast (>2000 km s{sup −1}) CMEs and ground level enhancements have harder or flatter spectra at low and high energies, and O break energies between ∼1 and 10 MeV nucleon{sup −1}. The latter events are enriched in {sup 3}He and higher-energy Fe, and have Fe spectra that rollover at significantly lower energies compared with O, probably because Fe ions with smaller Q/M ratios can escape from the distant shock more easily than O ions with larger Q/M ratios. We conclude that SEP spectral properties result from many complex and competing effects, namely Q/M-dependent scattering, shock properties, and the origin of the seed populations, all of which must be taken into account to develop a comprehensive picture of CME-driven shock acceleration of large gradual SEP events.

  3. Neutron-decay Protons from Solar Flares as Seed Particles for CME-shock Acceleration in the Inner Heliosphere

    Science.gov (United States)

    Murphy, Ronald J.; Ko, Yuan-Kuen

    2017-09-01

    The protons in large solar energetic particle events are accelerated in the inner heliosphere by fast shocks produced by coronal mass ejections. Unless there are other sources, the protons these shocks act upon would be those of the solar wind (SW). The efficiency of the acceleration depends on the kinetic energy of the protons. For a 2000 km s-1 shock, the most effective proton energies would be 30-100 keV; I.e., within the suprathermal tail component of the SW. We investigate one possible additional source of such protons: those resulting from the decay of solar-flare-produced neutrons that escape from the Sun into the low corona. The neutrons are produced by interactions of flare-accelerated ions with the solar atmosphere. We discuss the production of low-energy neutrons in flares and their decay on a interplanetary magnetic field line near the Sun. We find that even when the flaring conditions are optimal, the 30-100 keV neutron-decay proton density produced by even a very large solar flare would be only about 10% of that of the 30-100 keV SW suprathermal tail. We discuss the implication of a seed-particle source of more frequent, small flares.

  4. PS injection area

    CERN Multimedia

    1974-01-01

    Looking against the direction of protons in the main ring (left): the beam coming from the linac 1 either goes to the booster (on the right) or is deflected towards the PS to be directly injected into section 26 (facing the camera). Also shown the start of the TT2 line, ejected from straight section 16 to go towards the ISR passing over the beam line from the linac. (see Photo Archive 7409009)

  5. PS injection area

    CERN Multimedia

    1974-01-01

    To the right is the PS ring viewed along the direction of the protons. At the left the injection line coming from the 50 MeV Linac 1 (bottom) and going towards the 800 MeV booster, or deflected to the right to be injected directly into straight section 16. The drumlike element behind the (blue) dipole magnet is a 'debuncher' (a 200 MHz cavity). See photos 7409014X and 7409009.

  6. Micro-seeding and soft template effects on the control of polymorph and morphology of HMX micro particles in solvent-antisolvent process

    Directory of Open Access Journals (Sweden)

    Sajjad Damiri

    2017-12-01

    Full Text Available A seeding strategy was developed in the preparation of cyclotetramethylenetetranitramine (HMX explosive micro-particles by solvent-antisolvent method, to control their polymorphs from dangerous gamma (γ type to the desired and standard beta (β form with the size distribution of <10.0 μm, by using a low concentration of β-HMX fine particles as micro-seed in the antisolvent medium. All products were characterized by X-ray diffraction (XRD, scanning electron microscopy (SEM, and dynamic light scattering particle size analyzer. In the next step, the effective factors on the sizes and morphologies of micro-particles in the presence and absence of two soft templates of poly (ethylene glycol-400 (PEG-400 polymer and coconut fatty acid diethanolamide (lauramide surfactant were investigated. The results of experiments showed that using of water-soluble PEG-400 in the low antisolvent temperatures leads to the production of very spherical particles. Also non-ionic surfactant of lauramide, direct the crystal growth to needle-like structures. The advantages of this method are its capability for the simple production of β-HMX micro-particles in the large scale production process, with the various crystal structures and particles size distributions.

  7. At PS170 (APPLE)

    CERN Multimedia

    CERN PhotoLab

    1983-01-01

    APPLE stands for Antiproton-Proton to Pair of LEptons (an acronym of the ancestor experiment PAPLEP), the PS170 experiment setup at LEAR to study e+e-pair production in antiproton-proton annihilation by Padova-(CEN) Saclay- Torino Collaboration. It consisted of a liquid hydrogen target surrounded by several layers of proportional chambers in the vertical field of a C-magnet (this photo), a gas Cerenkov counter, wire chambers, hodoscopes, and an electromagnetic calorimeter (see photo 8302539X, 8302540X). See also photo 8301539X for the setup assembly at an early stage.

  8. Measuring target for the PS Booster

    CERN Multimedia

    1971-01-01

    The measuring target for the PS Booster (originally 800 MeV, now 1.4 GeV). It measures the size of the beam by destroying all particles with amplitudes greater than the size of the fork, the position and width of which are adjustable. The plunging time is only 20 ms and the acceleration at the tip of the fork reaches 90 g. The servo-controlled linear motor is shown detached from the mechanism. See also 7602008.

  9. Memories of the PS and of LEP

    CERN Document Server

    Steinberger, Jack

    2012-01-01

    The CERN PS, which started in 1959, and the Brookhaven AGS in 1960, represented an advance by a factor of more than five in the energy of proton accelerators, from the 5 GeV of the Berkeley Bevatron to about 30 GeV. These accelerators made possible the large progress in our understanding of particles and their interactions over the next two decades, culminating in the electroweak and QCD gauge theories.

  10. Beyond iPS!

    Directory of Open Access Journals (Sweden)

    Editorial

    2012-01-01

    Full Text Available It’s undoubtedly a jubilant moment for scientists and clinicians working in the stem cell arena as Prof. Gurdon and Prof. Shinya Yamanaka have been chosen for the Nobel Prize in Physiology & Medicine this year. The mystery of cell biology is something unfathomable and probably the work of this duo as well as the other scientists, who have put their hands on in- vitro de-differentiation have opened our eyes to a new window or a new paradigm in cell biology. The iPS invention has brought a lot of hope in terms of potential direct benefits to treat several diseases, which have no definite options at the moment. But, we envisage that several spin-offs could come out of this invention and one very significant spin-off finding recently witnessed is the finding by Prof. Masaharu Seno and his team of researchers at the Okayama University, Japan (Chen L, et al. 2012, PLoS ONE 7(4:e33544.doi:10.1371/journal.pone.0033544. According to Prof. Seno, mouse iPS cells (miPS when cultured in the conditioned medium derived from cancer cell lines, differentiate into cancer stem cells (CSCs. While differentiating into CSCs, they do retain the potential to develop endothelial progenitor cells. Several questions arise here: 1.Are these miPS derived CSCs really pluripotent, even if the terminal differentiation destined to specific phenotypes? 2.Shouldn’t the Cancer Stem Cells be termed as cancer progenitor cells, as till date they are considered to be producing only cancer cells but not pluripotent to yield other types of normal tissues? The spin-offs could be infinite as the process of differentiation and de-differentiation happening due to trillions of signals and pathways, most still remaining not-so-well understood. A special mention should be made to Prof. Shinya Yamanaka as he has several sterling qualities to be a role-model for budding scientists. Apart from his passion for science, which made him shift his career from orthopedics to a cell biologist, his

  11. Secondary Organic Aerosol Coating Formation and Evaporation: Chamber Studies Using Black Carbon Seed Aerosol and the Single-Particle Soot Photometer

    OpenAIRE

    Metcalf, Andrew R.; Loza, Christine L.; Coggon, Matthew M.; Craven, Jill S; Haflidi H. Jonsson; Flagan, Richard C; Seinfeld, John H.

    2013-01-01

    The article of record as published may be found at http://dx.doi.org/10.1080/02786826.2012.750712 We report a protocol for using black carbon (BC) aerosol as the seed for secondary organic aerosol (SOA) formation in an environmental chamber. We employ a single-particle soot photometer (SP2) to probe single-particle SOA coating growth dynamics and find that SOA growth on nonspherical BC aerosol is diffusion- limited. Aerosol composition measurements with an Aerodyne high resolution time-of-...

  12. PS wire chamber

    CERN Multimedia

    1970-01-01

    A wire chamber used at CERN's Proton Synchrotron accelerator in the 1970s. Multi-wire detectors contain layers of positively and negatively charged wires enclosed in a chamber full of gas. A charged particle passing through the chamber knocks negatively charged electrons out of atoms in the gas, leaving behind positive ions. The electrons are pulled towards the positively charged wires. They collide with other atoms on the way, producing an avalanche of electrons and ions. The movement of these electrons and ions induces an electric pulse in the wires which is collected by fast electronics. The size of the pulse is proportional to the energy loss of the original particle.

  13. SPS and PS Experiments Committee

    CERN Multimedia

    CERN. Geneva

    2004-01-01

    OPEN SESSION: 09:00 Status report of NA58 / COMPASS: A. Magnon 09:40 Status report of PS212 / DIRAC: L. Tausher 10:10 PS212 / DIRAC Addendum: L. Nemenov CLOSED SESSION on Tuesday, 27 April 2004 after the open session, Main Building, 6th floor conference room

  14. Particle trajectories in seeds of Lactuca sativa and chromosome aberrations after exposure to cosmic heavy ions on cosmos biosatellites 8 and 9

    Science.gov (United States)

    Facius, R.; Scherer, K.; Reitz, G.; Bücker, H.; Nevzgodina, L. V.; Maximova, E. N.

    1994-10-01

    The potentially specific importance of the heavy ions of the galactic cosmic radiation for radiation protection in manned spaceflight continues to stimulate in situ, i.e., spaceflight experiments to investigate their radiobiological properties. Chromosome aberrations as an expression of a direct assault on the genome are of particular interest in view of cancerogenesis being the primary radiation risk for man in space. In such investigations the establishment of the geometrical correlation between heavy ions' trajectories and the location of radiation sensitive biological substructures is an essential task. The overall qualitative and quantitative precision achieved for the identification of particle trajectories in the order of 2~10 μm as well as the contributing sources of uncertainties are discussed. We describe how this was achieved for seeds of Lactuca sativa as biological test organisms, whose location and orientation had to be derived from contact photographies displaying their outlines and those of the holder plates only. The incidence of chromosome aberrations in cells exposed during the COSMOS 1887 (Biosatellite 8) and the COSMOS 2044 (Biosatellite 9) mission was determined for seeds hit by cosmic heavy ions. In those seeds the incidence of both single and multiple chromosome aberrations was enhanced. The results of the Biosatellite 9 experiment, however, are confounded by spaceflight effects unrelated to the passage of heavy ions.

  15. Characterization of proanthocyanidin metabolism in pea (Pisum sativum) seeds.

    Science.gov (United States)

    Ferraro, Kiva; Jin, Alena L; Nguyen, Trinh-Don; Reinecke, Dennis M; Ozga, Jocelyn A; Ro, Dae-Kyun

    2014-09-16

    Proanthocyanidins (PAs) accumulate in the seeds, fruits and leaves of various plant species including the seed coats of pea (Pisum sativum), an important food crop. PAs have been implicated in human health, but molecular and biochemical characterization of pea PA biosynthesis has not been established to date, and detailed pea PA chemical composition has not been extensively studied. PAs were localized to the ground parenchyma and epidermal cells of pea seed coats. Chemical analyses of PAs from seeds of three pea cultivars demonstrated cultivar variation in PA composition. 'Courier' and 'Solido' PAs were primarily prodelphinidin-types, whereas the PAs from 'LAN3017' were mainly the procyanidin-type. The mean degree of polymerization of 'LAN3017' PAs was also higher than those from 'Courier' and 'Solido'. Next-generation sequencing of 'Courier' seed coat cDNA produced a seed coat-specific transcriptome. Three cDNAs encoding anthocyanidin reductase (PsANR), leucoanthocyanidin reductase (PsLAR), and dihydroflavonol reductase (PsDFR) were isolated. PsANR and PsLAR transcripts were most abundant earlier in seed coat development. This was followed by maximum PA accumulation in the seed coat. Recombinant PsANR enzyme efficiently synthesized all three cis-flavan-3-ols (gallocatechin, catechin, and afzalechin) with satisfactory kinetic properties. The synthesis rate of trans-flavan-3-ol by co-incubation of PsLAR and PsDFR was comparable to cis-flavan-3-ol synthesis rate by PsANR. Despite the competent PsLAR activity in vitro, expression of PsLAR driven by the Arabidopsis ANR promoter in wild-type and anr knock-out Arabidopsis backgrounds did not result in PA synthesis. Significant variation in seed coat PA composition was found within the pea cultivars, making pea an ideal system to explore PA biosynthesis. PsANR and PsLAR transcript profiles, PA localization, and PA accumulation patterns suggest that a pool of PA subunits are produced in specific seed coat cells early in

  16. The PS Booster Fast Wire Scanner

    CERN Document Server

    Burger, S; Priestnall, K; Raich, U

    2003-01-01

    The very tight emittance budget for LHC type beams makes precise emittance measurements in the injector complex a necessity. The PS machine uses 2 fast wire scanners per transverse plane for emittance measurement of the circulating beams. In order to ease comparison the same type of wire scanners have been newly installed in the upstream machine, the PS Booster, where each of the 4 rings is equipped with 2 wire scanners measuring the horizontal and vertical profiles. Those wire scanners use new and more modern control and readout electronics featuring dedicated intelligent motor movement controllers, which relieves the very stringent real time constraints due to the very high speed of 20m/s. In order to be able to measure primary beams at the very low injection energy of the Booster (50MeV) secondary emission currents from the wire can be measured as well as secondary particle flows at higher primary particle energies during and after acceleration. The solution adopted for the control of the devices is descri...

  17. The new heart of the PS is beating strongly

    CERN Document Server

    Corinne Pralavorio

    2011-01-01

    The PS has resumed operation with a brand new electrical power system called POPS; this enormous system comprising power electronics and capacitors is crucial because if it broke down practically no particles would be able to circulate at CERN. As soon as it started, POPS passed all the tests with flying colours and is now pulsing at full power.   The new PS power system is made up of 6 containers, each with 60 tonnes of capacitors and 8 power converters. The date 11/02/11 will always be remembered with affection by the engineers in the Electrical Power Converters Group. At 11:11 in the morning (no joke), the first beams powered by the new system began to circulate in the PS. The cutely-named POPS (POwer for PS) took over from the old rotating machine that had been working since 1968. From now on it will be POPS that supplies the PS main magnets with the electrical pulses needed to accelerate the beams for the LHC and all CERN's other facilities. The system is crucial as the PS is one of the lyn...

  18. Stability of a Lipase Extracted from Seeds of Pachira aquatica in Commercial Detergents and Application Tests in Poultry Wastewater Pretreatment and Fat Particle Hydrolysis

    Directory of Open Access Journals (Sweden)

    Patrícia Peres Polizelli

    2013-01-01

    Full Text Available A protein extract containing a plant lipase from oleaginous seeds of Pachira aquatica was tested using soybean oil, wastewater from a poultry processing plant, and beef fat particles as substrate. The hydrolysis experiments were carried out at a temperature of 40°C, an incubation time of 90 minutes, and pH 8.0-9.0. The enzyme had the best stability at pH 9.0 and showed good stability in the alkaline range. It was found that P. aquatica lipase was stable in the presence of some commercial laundry detergent formulations, and it retained full activity up to 0.35% in hydrogen peroxide, despite losing activity at higher concentrations. Concerning wastewater, the lipase increased free fatty acids release by 7.4 times and promoted the hydrolysis of approximately 10% of the fats, suggesting that it could be included in a pretreatment stage, especially for vegetable oil degradation.

  19. The PS locomotive runs again

    CERN Multimedia

    2001-01-01

    Over forty years ago, the PS train entered service to steer the magnets of the accelerator into place... ... a service that was resumed last Tuesday. Left to right: Raymond Brown (CERN), Claude Tholomier (D.B.S.), Marcel Genolin (CERN), Gérard Saumade (D.B.S.), Ingo Ruehl (CERN), Olivier Carlier (D.B.S.), Patrick Poisot (D.B.S.), Christian Recour (D.B.S.). It is more than ten years since people at CERN heard the rumbling of the old PS train's steel wheels. Last Tuesday, the locomotive came back into service to be tested. It is nothing like the monstrous steel engines still running on conventional railways -just a small electric battery-driven vehicle employed on installing the magnets for the PS accelerator more than 40 years ago. To do so, it used the tracks that run round the accelerator. In fact, it is the grandfather of the LEP monorail. After PS was commissioned in 1959, the little train was used more and more rarely. This is because magnets never break down, or hardly ever! In fact, the loc...

  20. The PS Booster hits 40

    CERN Multimedia

    Joannah Caborn Wengler

    2012-01-01

    Many accelerators’ "round" birthdays are being celebrated at CERN these days – the PS turned 50 in 2009, the SPS was 35 in 2011, and this year it's the turn of the PS Booster to mark its 40th anniversary. Originally designed to accelerate 1013 protons to 800 MeV, it has far exceeded its initial design performance over the years.   The PS Booster in the 1970s. Imagine the scene: a group of accelerator physicists staring expectantly at a monitor, when suddenly a shout of joy goes up as a signal flickers across the screen. Does that sound familiar? Well, turn the clock back 40 years (longer hair, wider trouser legs) and you have the situation at the PS Booster on 26 May 1972. On that day, beam was injected into the Booster for the first time. “It was a real buzz,” says Heribert Koziol, then Chairman of the Running-in Committee. “We were very happy – and also a little relieved – when the beam finally...

  1. Microfabricated device for co-culture of sympathetic neuron and iPS-derived cardiomyocytes.

    Science.gov (United States)

    Takeuchi, Akimasa; Shimba, Kenta; Takayama, Yuzo; Kotani, Kiyoshi; Lee, Jong-Kook; Noshiro, Makoto; Jimbo, Yasuhiko

    2013-01-01

    Induced pluripotent stem (iPS) cell-derived cardiomyocytes (iPS-CMs) has been expected as a cell source for therapy of serious heart failure. However, it is unclear whether the function of iPS-CMs is modulated by the host sympathetic nervous system. Here we developed a device for co-culture of sympathetic neurons and iPS-CMs using microfabrication technique. The device consisted of a culture chamber and a microelectrode-array (MEA) substrate. The superior cervical ganglion (SCG) neurons were co-cultured with iPS-CMs in a microfabricated device, which had multiple compartments. Several days after seeding, synapses were formed between SCG neurons and iPS-CMs, as confirmed by immunostaining. Spontaneous electrical activities of the SCG neurons and the iPS-CMs were observed from the electrode of the MEA substrate. The beat rate of iPS-CMs increased after electrical stimulation of the co-cultured SCG neurons. Such changes in the beat rate were prevented in the presence of propranolol, a β-adrenoreceptor antagonist. These results suggest that the microfabricated device will be utilized for studying the functional modulation of iPS-CMs by connected sympathetic neurons.

  2. A Continuous Flow Diffusion Chamber Study of Sea Salt Particles Acting as Cloud Seeds: Deliquescence, Ice Nucleation and Sublimation

    Science.gov (United States)

    Kong, X.; Wolf, M. J.; Garimella, S.; Roesch, M.; Cziczo, D. J.

    2016-12-01

    Sea Salt Aerosols (SSA) are abundant in the atmosphere, and important to the Earth's chemistry and energy budget. However, the roles of sea salts in the context of cloud formation are still poorly understood, which is partially due to the complexity of the water-salt phase diagram. At ambient temperatures, even well below 0°C, SSA deliquesces at sub-water saturated conditions. Since the ratio of the partial pressure over ice versus super-cooled water continuously declines with decreasing temperatures, it is interesting to consider if SSA continues to deliquesce under a super-saturated condition of ice, or if particles act as depositional ice nuclei when a critical supersaturation is reached. Some recent studies suggest hydrated NaCl and simulated sea salt might deliquesce between -35°C to -44°C, and below that deposition freezing becomes possible. Deliquesced droplets can subsequently freeze via the immersion or homogenous freezing mode, depending on if the deliquescence processes is complete. After the droplets or ice particles are formed, it is also interesting to consider how the different processes influence physical properties after evaporation or sublimation. This data is important for climate modeling that includes bromine burst observed in Antarctica, which is hypothesized to be relevant to the sublimation of blowing snow particles. In this study we use a SPectrometer for Ice Nuclei (SPIN; DMT, Inc., Boulder, CO) to perform experiments over a wide range of temperature and RH conditions to quantify deliquescence, droplet formation and ice nucleation. The formation of droplets and ice particles is detected by an advanced Optical Particle Counter (OPC) and the liquid/solid phases are distinguished by a machine learning method based on laser scattering and polarization data. Using an atomizer, four different sea salt samples are generated: pure NaCl and MgCl2 solutions, synthetic seawater, and natural seawater. Downstream of the SPIN chamber, a Pumped

  3. A&T Sector Note on the PS transverse feedback

    CERN Document Server

    Coly, Marcel; Blas, Alfred; Sterbini, Guido; CERN. Geneva. ATS Department

    2017-01-01

    In a particle accelerator, several contributions can degrade the beam quality and particularly the beam transverse emittance. In this document we will describe a system used in the CERN Proton Synchrotron (PS) to cope with the injection steering errors and the transverse instabilities: the PS transverse feedback (PS TFB). As time progresses, this system is also being used for other purpose, to increase in a controlled way the beam transverse emittance and to excite the beam for the Multi-Turn-Extraction (MTE). In 2016, it has been successfully used on some operational beams to damp injection oscillations. This allowed to test the reliability of the system for its operational deployment. A piquet service is available in case of problem.

  4. The HARP detector at the CERN PS

    CERN Document Server

    Catanesi, M G; Radicioni, E; Simone, S; Edgecock, R; Ellis, M; Robbins, S; Soler, F J P; Gößling, C; Mass, M; Bunyatov, S; Chukanov, A; Klimov, O; Krasin, I; Krasnoperov, A; Kustov, D; Popov, B; Serdiouk, V; Tereshchenko, V; Carassiti, V; Di Capua, E; Evangelisti, F; Vidal-Sitjes, G; Artamonov, A; Arce, P; Brocard, R; Decreuse, G; Friend, B; Giani, S; Gilardoni, S; Gorbunov, p; Grant, A; Grossheim, A; Gruber, P; Ivanchenko, V; Legrand, J C; Kayis-Topaksu,A; Panman, P; Papadopoulos, I; Pasternak, J; Chernyaev, E; Tsukerman, I; van der Vlugt, R; Veenhof, R; Wiebusch, C; Zucchelli, P; Blondel, A; Borghi, S; Campanelli, M; Cervera-Villanueva, A; Morone, M C; Prior, G; Schroeter, R; Kato, I; Gastaldi, Ugo; Mills, G B; Graulich, J S; Grégoire, G; Bonesini, M; Chignoli, F; Ferri, F; Paleari, F; Kirsanov, M; Postoev, V; Bagulya A; Grichine, V; Polukhina, N; Palladino, V; Coney, L; Schmitz, D; Barr, G; De Santo, A; Pattison, C; Zuber, K; Barichello, G; Bobisut, F; Gibin, D; Guglielmi, A; Laveder, M; Menegolli, A; Mezzetto M; Pepato, Adriano; Dumarchez, J; Troquereau, S; Vannucci, F; Dore, U; Iaciofano, A; Lobello, M; Marinilli, F; Orestano, D; Panayotov, D; Pasquali, M; Pastore, F; Tonazzo, A; Tortora, L; Booth, C; Buttar, C; Hodgson, P; Howlett, L; Nicholson, R; Bogomilovw, M; Burin, K; Chizhov, M; Kolev, D; Petev, P; Rusinov, I; Tsenov, R; Piperov, S; Temnikov, P; Apollonio, M; Chimenti, P; Giannini, G; Santin, G; Burguet-Castell, J; Gómez-Cadenas, J J; Novella, P; Sorel, M; Tornero, A

    2007-01-01

    HARP is a high-statistics, large solid angle experiment to measure hadron production using proton and pion beams with momenta between 1.5 and 15 GeV/c impinging on many different solid and liquid targets from low to high Z. The experiment, located in the T9 beam of the CERN PS, took data in 2001 and 2002. For the measurement of momenta of produced particles and for the identification of particle types, the experiment includes a large-angle spectrometer, based on a Time Projection Chamber and a system of Resistive Plate Chambers, and a forward spectrometer equipped with a set of large drift chambers, a threshold Cherenkov detector, a time-of-flight wall and an electromagnetic calorimeter. The large angle system uses a solenoidal magnet, while the forward spectrometer is based on a dipole magnet. Redundancy in particle identification has been sought, to enable the cross-calibration of efficiencies and to obtain a few percent overall accuracy in the cross-section measurements. Detector construction, operation an...

  5. Boiling treatment of ABS and PS plastics for flotation separation.

    Science.gov (United States)

    Wang, Chong-qing; Wang, Hui; Wu, Bao-xin; Liu, Qun

    2014-07-01

    A new physical method, namely boiling treatment, was developed to aid flotation separation of acrylonitrile-butadiene-styrene (ABS) and polystyrene (PS) plastics. Boiling treatment was shown to be effective in producing a hydrophilic surface on ABS plastic. Fourier Transform Infrared analysis was conducted to investigate the mechanism of boiling treatment of ABS. Surface rearrangement of polymer may be responsible for surface change of boiling treated ABS, and the selective influence of boiling treatment on the floatability of boiling treated plastics may be attributed to the difference in the molecular mobility of polymer chains. The effects of flotation time, frother concentration and particle size on flotation behavior of simple plastic were investigated. Based on flotation behavior of simple plastic, flotation separation of boiling treatment ABS and PS with different particle sizes was achieved efficiently. The purity of ABS and PS was up to 99.78% and 95.80%, respectively; the recovery of ABS and PS was up to 95.81% and 99.82%, respectively. Boiling treatment promotes the industrial application of plastics flotation and facilitates plastic recycling. Copyright © 2014 Elsevier Ltd. All rights reserved.

  6. EDH 'Millionaire' in PS Division

    CERN Document Server

    2001-01-01

    Christmas cheer! Left to right: Gerard Lobeau receives a bottle of Champagne from Derek Mathieson and Jurgen De Jonghe in recognition of EDH's millionth document. At 14:33 on Monday 3 December a technician in PS division, Gerard Lobeau, unwittingly became part of an important event in the life of CERN's Electronic Document Handling system (EDH). While ordering some pieces of aluminum for one of the PS's 10Mhz RF cavities, he created EDH document number 1,000,000. To celebrate the event Derek Mathieson (EDH Project Leader) and Jurgen De Jonghe (Original EDH Project Leader) presented Mr Lobeau with a bottle of champagne. As with 93% of material requests, Mr Lobeau's order was delivered within 24 hours. 'I usually never win anything' said Mr Lobeau as he accepted his prize, 'I initially though there may have been a problem with EDH when the document number had so many zeros in it, and was then surprised to get a phone call from you a few minutes later.' The EDH team had been monitoring the EDH document number ...

  7. Seed quality in informal seed systems

    NARCIS (Netherlands)

    Biemond, P.C.

    2013-01-01

    Keywords:     informal seed systems, seed recycling, seed quality, germination, seed pathology, seed health, seed-borne diseases, mycotoxigenic fungi, Fusarium verticillioides, mycotoxins, Vigna unguiculata, Zea mays, Nigeria.   Seed is a crucial input for agricultural

  8. High-resolution fine mapping of ps-2, a mutated gene conferring functional male sterility in tomato due to non-dehiscent anthers

    NARCIS (Netherlands)

    Gorguet, B.J.M.; Schipper, E.H.; Heusden, van A.W.; Lindhout, P.

    2006-01-01

    Functional male sterility is an important trait for the production of hybrid seeds. Among the genes coding for functional male sterility in tomato is the positional sterility gene ps-2. ps-2 is monogenic recessive, confers non-dehiscent anthers and is the most suitable for practical uses. In order

  9. The role of a conserved membrane proximal cysteine in altering αPS2CβPS integrin diffusion

    Science.gov (United States)

    Syed, Aleem; Arora, Neha; Bunch, Thomas A.; Smith, Emily A.

    2016-12-01

    Cysteine residues (Cys) in the membrane proximal region are common post-translational modification (PTM) sites in transmembrane proteins. Herein, the effects of a highly conserved membrane proximal α-subunit Cys1368 on the diffusion properties of αPS2CβPS integrins are reported. Sequence alignment shows that this cysteine is palmitoylated in human α3 and α6 integrin subunits. Replacing Cys1368 in wild-type integrins with valine (Val1368) putatively blocks a PTM site and alters integrins’ ligand binding and diffusion characteristics. Both fluorescence recovery after photobleaching (FRAP) and single particle tracking (SPT) diffusion measurements show Val1368 integrins are more mobile compared to wild-type integrins. Approximately 33% and 8% more Val1368 integrins are mobile as measured by FRAP and SPT, respectively. The mobile Val1368 integrins also exhibit less time-dependent diffusion, as measured by FRAP. Tandem mass spectrometry data suggest that Cys1368 contains a redox or palmitoylation PTM in αPS2CβPS integrins. This membrane proximal Cys may play an important role in the diffusion of other alpha subunits that contain this conserved residue.

  10. Ps-atom scattering at low energies

    CERN Document Server

    Fabrikant, I I

    2015-01-01

    A pseudopotential for positronium-atom interaction, based on electron-atom and positron-atom phase shifts, is constructed, and the phase shifts for Ps-Kr and Ps-Ar scattering are calculated. This approach allows us to extend the Ps-atom cross sections, obtained previously in the impulse approximation [Phys. Rev. Lett. 112, 243201 (2014)], to energies below the Ps ionization threshold. Although experimental data are not available in this low-energy region, our results describe well the tendency of the measured cross sections to drop with decreasing velocity at $v<1$ a.u. Our results show that the effect of the Ps-atom van der Waals interaction is weak compared to the polarization interaction in electron-atom and positron-atom scattering. As a result, the Ps scattering length for both Ar and Kr is positive, and the Ramsauer-Townsend minimum is not observed for Ps scattering from these targets. This makes Ps scattering quite different from electron scattering in the low-energy region, in contrast to the inter...

  11. Enhanced personal protection at the PS

    CERN Multimedia

    Samuel Morier Genoud

    2013-01-01

    Pictures 03, 06, 07 08 : Pierre Ninin, deputy group leader of GS-ASE and responsible for the installation of the new PS complex safety system, in front of a new access control system.Pictures 10, 12 ,13 : View of Building 271, the future control centre of the new PS complex safety system.

  12. PS, SL and LHC Auditoria change names

    CERN Document Server

    2003-01-01

    Following the replacement of the PS, SL and LHC Divisions by the AB and AT Divisions, the Auditoria are also changing their names. PS Auditorium is renamed AB Meyrin SL Auditorium is renamed AB Prévessin LHC Auditorium is renamed AT

  13. Magnetic-seeding filtration

    Energy Technology Data Exchange (ETDEWEB)

    DePaoli, D.W.; Tsouris, C. [Oak Ridge National Lab., TN (United States); Yiacoumi, Sotira

    1997-10-01

    Magnetic-seeding filtration is a technology under development for the enhanced removal of magnetic and non-magnetic particulates from liquids. This process involves the addition of a small amount of magnetic seed particles (such as naturally occurring iron oxide) to a waste suspension, followed by treatment with a magnetic filter. Non-magnetic and weakly magnetic particles are made to undergo nonhomogeneous flocculation with the seed particles, forming flocs of high magnetic susceptibility that are readily removed by a conventional high-gradient magnetic filter. This technology is applicable to a wide range of liquid wastes, including groundwater, process waters, and tank supernatants. Magnetic-seeding filtration may be used in several aspects of treatment, such as (1) removal of solids, particularly those in the colloidal size range that are difficult to remove by conventional means; (2) removal of contaminants by precipitation processes; and (3) removal of contaminants by sorption processes. Waste stream characteristics for which the technology may be applicable include (1) particle sizes ranging from relatively coarse (several microns) to colloidal particles, (2) high or low radiation levels, (3) broad-ranging flow rates, (4) low to moderate solids concentration, (5) cases requiring high decontamination factors, and (6) aqueous or non-aqueous liquids. At this point, the technology is at the bench-scale stage of development; laboratory studies and fundamental modeling are currently being employed to determine the capabilities of the process.

  14. Secondary Organic Aerosol Coating Formation and Evaporation: Chamber Studies Using Black Carbon Seed Aerosol and the Single-Particle Soot Photometer

    National Research Council Canada - National Science Library

    Metcalf, Andrew R; Loza, Christine L; Coggon, Matthew M; Craven, Jill S; Jonsson, Haflidi H; Flagan, Richard C; Seinfeld, John H

    2013-01-01

    .... We employ a 3-wavelength photoacoustic soot spectrometer (PASS-3) to measure optical properties of the systems studied, including fullerene soot as the surrogate BC seed, nucleated naphthalene SOA from high-NOx photooxidation, and nucleated...

  15. On the crystallization behavior of syndiotactic-b-atactic polystyrene stereodiblock copolymers, atactic/syndiotactic polystyrene blends, and aPS/sPS blends modified with sPS-b-aPS

    Energy Technology Data Exchange (ETDEWEB)

    Annunziata, Liana, E-mail: liana.annunziatta@univ-rennes1.fr [Organométalliques et Catalyse, UMR 6226 Sciences Chimiques CNRS, Université de Rennes 1, Campus de Beaulieu, F-35042 Rennes Cedex (France); Monasse, Bernard, E-mail: bernard.monasse@mines-paristech.fr [Mines-ParisTech, CEMEF, Centre de Mise en Forme des Matériaux, UMR CNRS 7635, Sophia Antipolis (France); Rizzo, Paola; Guerra, Gaetano [Dipartimento di Chimica e Biologia, Università degli studi di Salerno, Via Ponte don Melillo, I-84084 Fisciano, SA (Italy); Duc, Michel [Total Petrochemicals Research Feluy, Zone Industrielle Feluy C, B-7181 Seneffe (Belgium); Carpentier, Jean-François, E-mail: jean-francois.carpentier@univ-rennes1.fr [Organométalliques et Catalyse, UMR 6226 Sciences Chimiques CNRS, Université de Rennes 1, Campus de Beaulieu, F-35042 Rennes Cedex (France)

    2013-09-16

    Crystallization and morphological features of syndiotactic-b-atactic polystyrene stereodiblock copolymers (sPS-b-aPS), atactic/syndiotactic polystyrene blends (aPS/sPS), and aPS/sPS blends modified with sPS-b-aPS, with different compositions in aPS and sPS, have been investigated using differential scanning calorimetry (DSC), polarized light optical microscopy (POM) and wide angle X-ray diffraction (WAXRD) techniques. For comparative purposes, the properties of parent pristine sPS samples were also studied. WAXRD analyses revealed for all the samples, independently from their composition (aPS/sPS ratio) and structure (blends, block copolymers, blends modified with block copolymers), the same polymorphic β form of sPS. The molecular weight of aPS and sPS showed opposite effects on the crystallization of 50:50 aPS/sPS blends: the lower the molecular weight of aPS, the slower the crystallization while the lower the molecular weight of sPS, the faster the crystallization. DSC studies performed under both isothermal and non-isothermal conditions, independently confirmed by POM studies, led to a clear trend for the crystallization rate at a given sPS/aPS ratio (ca. 50:50 and 20:80): sPS homopolymers > sPS-b-aPS block copolymers ∼sPS/aPS blends modified with sPS-b-aPS copolymers > sPS/aPS blends. Interestingly, sPS-b-aPS block copolymers not only crystallized faster than blends, but also affected positively the crystallization behavior of blends. At 50:50 sPS/aPS ratio, blends (Blend-2), block copolymers (Cop-1) and blends modified with block copolymers (Blend-2-mod) crystallized via spherulitic crystalline growth controlled by an interfacial process. In all cases, an instantaneous nucleation was observed. The density of nuclei in block copolymers (160,000−190,000 nuclei mm{sup −3}) was always higher than that in blends and modified blends (30,000−60,000 nuclei mm{sup −3}), even for quite different sPS/aPS ratio. At 20:80 sPS/aPS ratio, the block copolymers

  16. Molecular Cloning and Expression Analysis of Cryptochrome Gene PsCRY2 in Tree Peony

    Directory of Open Access Journals (Sweden)

    Xiuxia REN

    2016-11-01

    Full Text Available Cryptochromes are blue/ultraviolet-A (UV-A light receptors involved in regulating various aspects of plant growth and development. Investigations of the structure and functions of cryptochromes in plants have largely focused on herbaceous plants. However, few data on the function of CRY2 are available in woody plants. In this study, a cryptochrome 2 (CRY2 gene was isolated from Paeonia suffruticosa by Reverse Transcription Polymerase Chain Reaction (RT-PCR. Sequence alignment and motif analysis showed that the deduced amino acids contained a PHR domain near the amino terminus and a CCT domain near the carboxy terminus. PsCRY2 showed high identity with AtCRY2 of Arabidopsis. Phylogenetic analysis indicated that it was closely related to Citrus sinensis. Gene expression analysis revealed that the highest expression levels of PsCRY2 occurred in the bud and seed embryo of P. suffruticosa, followed by the roots, stems, and leaves. PsCRY2 was upregulated during the entire process of bud differentiation, whereas this was downregulated during the early stage of bud development and upregulated in the middle and late stages. The highest level of PsCRY2 expression was observed in the big bell-like flower buds. These results suggested that PsCRY2 plays an important role in both bud differentiation and bud development. The expression patterns of PsCRY2 in the buds of ‘Luoyanghong’ and ‘Qiufa 1’ were similar, whereas that in the buds of ‘Qiufa 1’ was significantly higher than in the buds of ‘Luoyanghong’. The buds of plants subjected to different photoperiod treatments exhibited variations in PsCRY2 expression patterns. The expression of PsCRY2 decreased during bud sprouting and in the small bell-like flower buds that were subjected to short-day photoperiod compared to that observed under long-day photoperiod.

  17. Ps 22 in Gospels’ interpretation of Passion

    Directory of Open Access Journals (Sweden)

    Sylwester Jędrzejewski

    2012-09-01

    Full Text Available Ps 22 is a piece of artistically high poetry, clear images and metaphors, historical and prophetic references. The conviction of biblical scholars that the New Testament writers has recognized in Ps 22 prophetic witness of passion, accompanies the Church from its beginnings. The words of Jesus on the cross, taken from Ps 22: 2, have a character of lamentable re-symbolization of the prayer of Israel. These words establish a theological answer in the form of suitable credo as well. Dramatic question “why?” is connected with a proclamation and identification “My God”. The personal experience of oppression and death is included by Jesus in the history of his nation and in the experience of God. Ps 22 in the Gospels’ passion context becomes a proclamation form of prayer and a very personal, expressed in such dramatic circumstances confession of the faith.

  18. Compressed 6 ps pulse in nonlinear amplification of a Q-switched microchip laser

    Science.gov (United States)

    Diao, Ruxin; Liu, Zuosheng; Niu, Fuzeng; Wang, Aimin; Taira, Takunori; Zhang, Zhigang

    2017-02-01

    We present a passively Q-switched Nd:YVO4 crystal microchip laser with a 6 ps pulse width, which is based on SPM-induced spectral broadening and pulse compression. The passive Q-switching is obtained by a semiconductor saturable absorber mirror. The laser’s seed source centered at 1064 nm pulses with a duration of 80 ps, at a repetition rate of 600 kHz corresponding to an average output power of 10 mW. After amplification and compression, the pulses were compressed to 6 ps with a maximum pulse energy of 0.5 µJ.

  19. Yasp for LEIR to PS injection

    CERN Document Server

    Kain, V; Bartosik, H; Huschauer, A; Jacquet, D; Nicosia, D; Pasinelli, S; Wenninger, J

    2017-01-01

    The steering program YASP was introduced in the LEIRinjection as well as the extraction lines in 2016 to correctthe trajectories with well-known model based correctionalgorithms such as MICADO or SVD. In addition a YASPconfiguration was prepared to correct the extraction linetogether with the first turn of the PS. In this way the injectionoscillations can be corrected while keeping the trajectoryreasonable in the PS injection line.

  20. PS overcomes two serious magnet failures

    CERN Multimedia

    Maximilien Brice

    2003-01-01

    Two magnets and a bus bar connection in the PS were found to be faulty during high-voltage tests at the end of the accelerator shutdown. A five-week repair schedule was quickly devised. A team of mechanics, technicians and engineers worked at full speed to replace the faulty magnets, succeeding in limiting the delay of the accelerators' spring start-up to two weeks. Here we see the PS magnet string awaiting the replacement no. 6 magnet.

  1. Confinement Effect Emission from Infiltrated ZnO in PS-b-PMMA Nanostructures

    OpenAIRE

    Blaisdell-Pijuan, Paris L.; Gosztola, David J.; Yanguas-Gil, Angel; Ren, Jiaxing; Nealey, Paul; Ma, Xuedan; Gray, Stephen; Ocola, Leonidas E.

    2017-01-01

    We have characterized the growth of ZnO using sequential infiltration synthesis (SiS) on PS-b-PMMA block copolymers (BCP) of spherical and cylindrical sub-20nm morphologies and studied how the photoluminescence of these nanostructures varies per its seed layer. Investigation of these structures was done using atomic force microscopy (AFM), spectrofluorometry, Raman spectroscopy, and scanning electron microscopy (SEM). We report blue-shifted photoemission at 335 nm (3.70 eV), suggesting quantu...

  2. Low energy o-Ps-o-Ps elastic scattering using a simple model

    Energy Technology Data Exchange (ETDEWEB)

    Himanshu, Sharma [Veer Kunwar Singh Univ., Dept. of Physics, Bihar (India); Kiran, Kumari [R N College, P. G. Dept. of Physics, Bihar (India); Sumana, Chakraborty [Indian Association for the Cultivation of Science, Dept. of Theoretical Physics (India)

    2009-06-15

    A simple model is employed to investigate o-Ps-o-Ps (positronium-positronium) scattering at low energies. This model contains the effect of exchange explicitly and a model long range potential in the framework of static-exchange model. These two physical features are of key importance in Ps-Ps (atom-atom) scattering system. S-wave triplet-triplet and singlet-singlet scattering lengths and corresponding phase shifts up to the incident momentum k = 0.5 a.u. are in excellent agreement with those yielded by most elaborate and theoretically sound predictions. (authors)

  3. Degradation of cyanogenic glycosides of bitter apricot seeds (Prunus armeniaca) by endogenous and added enzymes as affected by heat treatments and particle size.

    NARCIS (Netherlands)

    Tuncel, G.; Nout, M.J.R.; Brimer, L.

    1998-01-01

    Bitter apricot (Prunus armeniaca) seeds (kernels) are by-products of the apricot processing industry. They contain approximately 50-150 μMol/g (dry weight basis) of potentially toxic cyanogenic glycosides, mainly amygdalin and prunasin. The present paper deals with the degradation of these

  4. Seed quality in informal seed systems

    OpenAIRE

    Biemond, P.C.

    2013-01-01

    Keywords:     informal seed systems, seed recycling, seed quality, germination, seed pathology, seed health, seed-borne diseases, mycotoxigenic fungi, Fusarium verticillioides, mycotoxins, Vigna unguiculata, Zea mays, Nigeria.   Seed is a crucial input for agricultural production. Approximately 80% of the smallholder farmers in Africa depend for their seed on the informal seed system, consisting of farmers involved in selection, production and dissemination of seed. The la...

  5. LS1 Report: PS beams are back!

    CERN Multimedia

    Katarina Anthony & Anaïs Schaeffer

    2014-01-01

    For the first time in over 15 months, there are beams back in the PS. Making their first tour of the accelerator today, 20 June, their injection marks the end of weeks of cold checkouts and hardware commissioning in the PS.   The CERN Control Centre (CCC) is back in business: people gather to restart the LHC injectors, today the PS. Since hardware commissioning was wrapped up on 23 May, the Operations Group (BE-OP) has been conducting cold checkouts on the PS. This involves switching on all of the machine's systems, verifying that they respond to commands by OP and ensuring they are calibrated to beam timings. "These verifications were done, in part, during the hardware commissioning dry runs," says Rende Steerenberg, PS section leader. "But the cold checkouts are on a much larger scale, as we act as if there is beam in the whole machine. We placed a full load on the controls system, cooling, networks, etc. in order to setup the accelerator in the most realis...

  6. Detectors and Concepts for sub-100 ps timing with gaseous detectors

    CERN Document Server

    Gonzalez-Diaz, D.

    2017-01-01

    We give a short compendium of the main ongoing detectors and concepts capable of performing accurate sub-100 ps timing at high particle fluxes and on large areas, through technologies based on gaseous media. We briefly discuss the state-of-the-art, technological limitations and prospects, and a new bizarre idea.

  7. Collision between two ortho-positronium (Ps) atoms: A four-body ...

    Indian Academy of Sciences (India)

    10,11]. It is again useful for its applications in technology [18,19]. The Ps–Ps scattering problem is very difficult to treat theoretically as it is a four-body Coulomb problem. At lower energies, the particles are highly correlated. The proper symmetry ...

  8. Particle size distribution control of Pt particles used for particle gun

    Science.gov (United States)

    Ichiji, M.; Akiba, H.; Nagao, H.; Hirasawa, I.

    2017-07-01

    The purpose of this study is particle size distribution (PSD) control of submicron sized Pt particles used for particle gun. In this report, simple reaction crystallization is conducted by mixing H2PtCl6 and ascorbic acid. Without the additive, obtained Pt particles have broad PSD and reproducibility of experiment is low. With seeding, Pt particles have narrow PSD and reproducibility improved. Additionally, mean particle diameter of 100-700 nm is controlled by changing seeding amount. Obtained particles are successfully characterized as Pt by XRD results. Moreover, XRD spectra indicate that obtained particles are polycrystals. These experimental results suggest that seeding consumed nucleation, as most nuclei attached on the seed surface. This mechanism virtually restricted nucleation to have narrow PSD can be obtained.

  9. Fabrication of polystyrene/agave particle biocomposites using ...

    Indian Academy of Sciences (India)

    ... as compared to PS matrix. Addition of fire retardant fillers such as magnesium hydroxide Mg(OH)2 and zinc borate lowered burning rate of PS composites considerably. Scanning electron microscopy (SEM) of tensile fracture surfaces of AN grafted AgP/PS composites showed better particle/matrix adhesion.

  10. PS overcomes two serious magnet failures

    CERN Multimedia

    Maximilien Brice

    2003-01-01

    Two magnets and a bus bar connection in the PS were found to be faulty during high-voltage tests at the end of the accelerator shutdown. A five-week repair schedule was quickly devised. A team of mechanics, technicians and engineers worked at full speed to replace the faulty magnets, succeeding in limiting the delay of the accelerators' spring start-up to two weeks. These pictures show one of the magnets (no. 19) on the PS locomotive brought back into service for the removal and replacement operations.

  11. Positron Annihilation in the Bipositronium Ps2

    Energy Technology Data Exchange (ETDEWEB)

    Bailey, David H.; Frolov, Alexei M.

    2005-07-01

    The electron-positron-pair annihilation in the bipositronium PS2 is considered. In particular, the two-, three-, one- and zero-photon annihilation rates are determined to high accuracy. The corresponding analytical expressions are also presented. Also, a large number of bound state properties have been determined for this system.

  12. The 4 Ps as a Guiding Perspective

    Science.gov (United States)

    Kalsbeek, David H.

    2013-01-01

    A 4 Ps perspective addresses immediate needs: to help institutions gain traction in their retention strategies by framing and reframing the challenges and the possible responses, by challenging some of the traditional mental models about retention that can distract or dilute those strategies, and by offering focus and coherence to institutional…

  13. 10th Anniversary P.S.

    CERN Multimedia

    Adams,J

    1969-01-01

    John Adams parle de la préhistoire du P.S. avec présentation des dias. Le DG B.Gregory prend la parole. Les organisateurs présentent sous la direction du "Prof.Ocktette"(?) un sketch très humoristique (p.e.existence de Quark etc.....)

  14. Back to work for the PS

    CERN Multimedia

    2006-01-01

    On 22 June, the PS's rotating machine started turning again for the first time since its enforced shutdown one month ago (see Bulletin No. 23-24/2006) - and the PS was back in operation the very next day! A team from Siemens worked their socks off, 6 days a week for one month (including public holidays), to repair the electrical power supply in collaboration with the AB/PO Group's Main Power Converters (MPC) Section. The generator's faulty rotor was dismantled and replaced by the renovated spare rotor. The multitude of electrical and mechanical connections together with the sheer weight of the rotor (80 tonnes) made this an extremely complex job. The AB/PO Group used the shutdown to test a back-up solution for the PS power supply. The accelerator was directly wired up to the 18 kV electrical network via a 13 MVA transformer, installed at the end of the 1970s but never used. This solution succeeded in bringing the PS back into operation but at limited energy and frequency. Just 14 GeV could be achieved, whic...

  15. The Effect of Film Thickness and TiO2 Content on Film Formation from PS/ TiO2 Nanocomposites Prepared by Dip-Coating Method

    National Research Council Canada - National Science Library

    M. Selin Sunay; Onder Pekcan; Saziye Ugur

    2012-01-01

    ...) was used for studying film formation from TiO2 covered nanosized polystyrene (PS) latex particles (320 nm). The effects of film thickness and TiO2 content on the film formation and structure properties of PS/TiO2 composites were studied...

  16. Simulation of Instability at Transition Energy with a New Impedance Model for CERN PS

    CERN Document Server

    Wang, Na; Biancacci, Nicolo; Migliorati, Mauro; Persichelli, Serena; Sterbini, Guido

    2016-01-01

    Instabilities driven by the transverse impedance are proven to be one of the limitations for the high intensity reach of the CERN PS. Since several years, fast single bunch vertical instability at transition energy has been observed with the high intensity bunch serving the neu-tron Time-of-Flight facility (n-ToF). In order to better understand the instability mechanism, a dedicated meas-urement campaign took place. The results were compared with macro-particle simulations with PyHEADTAIL based on the new impedance model developed for the PS. Instability threshold and growth rate for different longitu-dinal emittances and beam intensities were studied.

  17. Green feasible route preparation for PMMA vs PS: Its properties for photonic crystal application

    Science.gov (United States)

    Azman, Nursyafiqa; Kassim, Syara; Azwa, Rabiatul Addawiyah; Harun, Noor Aniza

    2017-09-01

    This paper discusses a novel way to make photonic crystal by using poly (methyl methacrylate) (PMMA) and polystyrene (PS) colloidal particle. Both synthesis were through surfactant free-emulsion polymerization. This synthesis is so-called "green process" by having water as a medium instead of using solvent. Conventional polymerization technique mostly uses surfactant as a stabilizer to complete the polymerization process. However, the presence of surfactant may contaminate to the end product and difficult to control the size of colloidal sphere. The detailed morphology of uniform size 386 nm PMMA vs 420 nm PS will be discussed for its further potential photonic application.

  18. seed oil

    African Journals Online (AJOL)

    Wara

    ABSTRACT. Neem seed oil from the neem tree (Azadiracta indica) finds wide usage one of which is its utilization for cosmetics particularly soap products. The chemical analysis of seed oil was carried out using the methods reported by AOAC (1998), Akpan et al., (2006) and Bassir, (1978) which revealed that it had.

  19. Developmental and seed aging mediated regulation of antioxidative genes and differential expression of proteins during pre- and post-germinative phases in pea.

    Science.gov (United States)

    Yao, Zhen; Liu, Lingwei; Gao, Feng; Rampitsch, Christof; Reinecke, Dennis M; Ozga, Jocelyn A; Ayele, Belay T

    2012-10-15

    Enzymatic antioxidant system plays an important role in maintaining seed vigor and regulating plant growth and development. It involves a number of enzymes that scavenge excessive reactive oxygen species (ROS) produced during seed aging and also modulate the level of these compounds during plant developmental processes. This study investigated the transcriptional regulation of enzymatic antioxidative capacity in pea during the pre- and post-germinative phases and in response to seed aging by analyzing the spatio-temporal expression of five antioxidative genes: PsAPX, PsSOD, PsGRcyt, PsGRcm and PsCAT. Transcripts of all these genes were found in mature dry seeds, embryo axes and cotyledons of germinating seeds, and cotyledons, roots and shoots of young seedlings. However, PsAPX and PsSOD were predominant and exhibited developmental regulation, suggesting that these genes play important roles in controlling the intracellular homeostasis of ROS for promoting cell elongation, and thereby embryo axis expansion and early seedling growth in pea. Accelerated aging of pea seeds led to reduction in seed viability and seedling growth, and this effect was correlated with substantial decrease in the transcriptional activation of the prominent antioxidative genes. Furthermore, our proteomic analysis indicated the association of seed aging with changes in the abundance of specific proteins, revealing additional mechanisms underlying seed aging in pea. Copyright © 2012 Elsevier GmbH. All rights reserved.

  20. Determination of Beam Intensity and Position in a Particle Accelerator

    CERN Document Server

    Kasprowicz, Grzegorz; Raich, Uli

    2011-10-04

    A subject of the thesis is conception, design, implementation, tests and deployment of new position measurement system of particle bunch in the CERN PS circular accelerator. The system is based on novel algorithms of particle position determination. The Proton Synchrotron accelerator (PS), installed at CERN†, although commissioned in 1959, still plays a central role in the production of beams for the Antiproton Decelerator, Super Proton Synchrotron, various experimental areas and for the Large Hadron Collider (LHC)‡. The PS produces beams of different types of particles, mainly protons, but also various species of ions. Almost all these particle beams pass through the PS. The quality of the beams delivered to the LHC has a direct impact on the effective luminosity, and therefore the performance of the instrumentation of the PS is of great importance. The old trajectory and orbit measurement system of the PS is dated back to 1988 and no longer fulfilled present day requirements. It used 40 beam posi...

  1. Determination of beam intensity and position in a particle accelerator

    CERN Document Server

    Kasprowicz, G

    2011-01-01

    A subject of the thesis is conception, design, implementation, tests and deployment of new position measurement system of particle bunch in the CERN PS circular accelerator. The system is based on novel algorithms of particle position determination. The Proton Synchrotron accelerator (PS), installed at CERN, although commissioned in 1959, still plays a central role in the production of beams for the Antiproton Decelerator, Super Proton Synchrotron, various experimental areas and for the Large Hadron Collider (LHC). The PS produces beams of different types of particles, mainly protons, but also various species of ions. Almost all these particle beams pass through the PS. The quality of the beams delivered to the LHC has a direct impact on the effective luminosity, and therefore the performance of the instrumentation of the PS is of great importance. The old trajectory and orbit measurement system of the PS is dated back to 1988 and no longer fulfilled present day requirements. It used 40 beam position monitors...

  2. Sub-10ps monolithic and low-power photodetector readout

    Energy Technology Data Exchange (ETDEWEB)

    Varner, Gary S.; Ruckman, Larry L.

    2009-02-20

    Recent advances in photon detectors have resulted in high-density imaging arrays that offer many performance and cost advantages. In particular, the excellent transit time spread of certain devices show promise to provide tangible benefits in applications such as Positron Emission Tomography (PET). Meanwhile, high-density, high-performance readout techniques have not kept on pace for exploiting these developments. Photodetector readout for next generation high event rate particle identification and time-resolved PET requires a highly-integrated, low-power, and cost-effective readout technique. We propose fast waveform sampling as a method that meets these criteria and demonstrate that sub-10ps resolution can be obtained for an existing device.

  3. Role of insulin receptor and insulin signaling on αPS2CβPS integrins' lateral diffusion.

    Science.gov (United States)

    Mainali, Dipak; Syed, Aleem; Arora, Neha; Smith, Emily A

    2014-12-01

    Integrins are ubiquitous transmembrane receptors with adhesion and signaling properties. The influence of insulin receptor and insulin signaling on αPS2CβPS integrins' lateral diffusion was studied using single particle tracking in S2 cells before and after reducing the insulin receptor expression or insulin stimulation. Insulin signaling was monitored by Western blotting for phospho-Akt expression. The expression of the insulin receptor was reduced using RNA interference (RNAi). After insulin receptor RNAi, four significant changes were measured in integrin diffusion properties: (1) there was a 24% increase in the mobile integrin population, (2) 14% of the increase was represented by integrins with Brownian diffusion, (3) for integrins that reside in confined zones of diffusion, there was a 45% increase in the diameter of the confined zone, and (4) there was a 29% increase in the duration integrins spend in confined zones of diffusion. In contrast to reduced expression of the insulin receptor, which alters integrin diffusion properties, insulin stimulation alone or insulin stimulation under conditions of reduced insulin receptor expression have minimal effects on altering the measured integrin diffusion properties. The differences in integrin diffusion measured after insulin receptor RNAi in the presence or absence of insulin stimulation may be the result of other insulin signaling pathways that are activated at reduced insulin receptor conditions. No change in the average integrin diffusion coefficient was measured for any conditions included in this study.

  4. Studies of Space Charge Effects in the Proposed CERN PS2

    Energy Technology Data Exchange (ETDEWEB)

    Qiang, Ji; /LBL, Berkeley; Ryne, Robert; /LBL, Berkeley; De Maria, Riccardo; /Brookhaven; Macridin, Alexandru; /Fermilab; Spentzouris, Panagiotis; /Fermilab; Papaphilippou, Yannis; /CERN; Wienands, Ulrich; /SLAC

    2012-06-22

    A new proton synchrotron, the PS2, is under design study to replace the current proton synchrotron at CERN for the LHC upgrade. Nonlinear space charge effects could cause significant beam emittance growth and particle losses and limit the performance of the PS2. In this paper, we report on studies of the potential space-charge effects at the PS2 using three-dimensional self-consistent macroparticle tracking codes, IMPACT, MaryLie/IMPACT, and Synergia. We will present initial benchmark results among these codes. Effects of space-charge on the emittance growth, especially due to synchrotron coupling, aperture sizes, initial painted distribution, and RF ramping scheme will also be discussed.

  5. Seed regulations and local seed systems

    NARCIS (Netherlands)

    Louwaars, N.

    2000-01-01

    Seed regulations have been introduced in most countries based on the development of formal seed production. Concerns about seed quality and about the varietal identity of the seeds have commonly led to seed laws. However, formal regulations are often inappropriate for informal seed systems, which

  6. Enhanced penetration of exogenous EPCs into brains of APP/PS1 transgenic mice

    Science.gov (United States)

    Yuan, Xiaoyang; Mei, Bin; Zhang, Le; Zhang, Cuntai; Zheng, Miao; Liang, Huifang; Wang, Wei; Zheng, Jie; Ding, Ling; Zheng, Kai

    2016-01-01

    The aim of this study was to investigate the repair function of exogenous Endothelial progenitor cells (EPCs) for brain microvascular damage of the APP/PS1 transgenic mouse model of Alzheimer’s disease (AD). This study used a density-gradient centrifugation method to isolate mononuclear cells (MNCs) from mouse bone marrow, which were subsequently seeded and cultured. Cells were characterized by morphology and detection of the surface markers CD34 and CD133 at different time points by immunofluorescence (IF) and flow cytometry (FCM). Then, EPCs were transfected with GFP adenoviral vectors (GFP-EPCs). Wild-type (WT) and APP/PS1 transgenic mice both received GFP-EPCs injection through the tail vein, and using a PBS buffer injection as the control. Seven days later, the animals’ brain tissue was isolated. Expression of GFP was detected by quantitative polymerase chain reaction (qPCR) and western-blot (WB), while the fluorescence of GFP within the brains of mice was observed under a fluorescence microscope. Higher mRNA and protein expression of GFP, accompanied with increased green fluorescence, were detected in the brain of GFP-EPCs-injected APP/PS1 mice, as compared with GFP-EPCs-injected WT mice. The results show that the APP/PS1 transgenic mouse model of AD exhibited enhanced penetration of exogenous EPCs into brains than the WT mice. PMID:27186272

  7. PS overcomes two serious magnet failures

    CERN Multimedia

    Maximilien Brice

    2003-01-01

    Two magnets (no.'s 6 and 19)and a bus bar connection in the PS were found to be faulty during high-voltage tests at the end of the accelerator shutdown. A five-week repair schedule was quickly devised. A team of mechanics, technicians and engineers worked at full speed to replace the faulty magnets, succeeding in limiting the delay of the accelerators' spring start-up to two weeks. Pictured here are members of the PS team with the replacement no. 6 magnet. From left to right: In the back row, Frédéric Roussel (Transport DBS), Yves Bernard (Transport DBS), Luc Moreno (Cegelec), Thierry Battimanza (Transport DBS), Raymond Brown (AB/ABP), Thomas Zickler (AT/MEL); at the front, Steven Southern (AT/VAC), Thierry Gaidon (Brun & Sorensen), Philippe Vidales (Cegelec), Daniel Aubert (Cegelec), Jerome Cachet (Transport DBS), Jose Manual Gomes de Faria (AT/MEL), Eric Page (AT/VAC).

  8. PS overcomes two serious magnet failures

    CERN Multimedia

    Maximilien Brice

    2003-01-01

    Two magnets and a bus bar connection in the PS were found to be faulty during high-voltage tests at the end of the accelerator shutdown. A five-week repair schedule was quickly devised. A team of mechanics, technicians and engineers worked at full speed to replace the faulty magnets, succeeding in limiting the delay of the accelerators´ spring start-up to two weeks. Here we see one of the replacement magnets (no. 19) being prepared.

  9. The PS overcomes two serious magnet failures

    CERN Multimedia

    2003-01-01

    Two magnets and a bus bar connection in the PS were found to be faulty during high-voltage tests at the end of the accelerator shutdown. A five-week repair schedule was quickly devised. A team of mechanics, technicians and engineers worked at full speed to replace the faulty magnets, succeeding in limiting the delay of the accelerators' spring start-up to two weeks.

  10. Motor-Generator Set, PS Main Supply

    CERN Multimedia

    CERN PhotoLab

    1983-01-01

    This is the "new" motor-generator set. It replaced the previous, original, one which had served from the PS start-up in 1959. Ordered in 1965, installed in 1967, it was brought into operation at the beginning of 1968. Regularly serviced and fitted with modern regulation and controls, it still serves at the time of writing (2006) and promises to serve for several more years, as a very much alive museum-piece. See also 6803016 and 0201010.

  11. PS overcomes two serious magnet failures

    CERN Multimedia

    Maximilien Brice

    2003-01-01

    Two magnets and a bus bar connection in the PS were found to be faulty during high-voltage tests at the end of the accelerator shutdown. A five-week repair schedule was quickly devised. A team of mechanics, technicians and engineers worked at full speed to replace the faulty magnets, succeeding in limiting the delay of the accelerators' spring start-up to two weeks. Here we see one of the replacement magnets (no. 6) being prepared.

  12. Enhanced personal protection system for the PS

    CERN Multimedia

    Caroline Duc

    2013-01-01

    During the first long shutdown (LS1) a new safety system will be installed in the primary beam areas of the PS complex in order to bring the standard of personnel radiation protection at the PS into line with that of the LHC.   Pierre Ninin, deputy group leader of GS-ASE and responsible for the installation of the new PS complex safety system, in front of a new access control system. The LHC access control systems are state-of-the-art, whereas those of the injection chain accelerators were running the risk of becoming obsolete. For the past two years a project to upgrade the access and safety systems of the first links in the LHC accelerator chain has been underway to bring them into compliance with nuclear safety standards. These systems provide the personnel with automatic protection by limiting access to hazardous areas and by ensuring that nobody is present in the areas when the accelerator is in operation. By the end of 2013, the project teams will ha...

  13. Green synthesis of seashell supported silver nanoparticles using Bunium persicum seeds extract: Application of the particles for catalytic reduction of organic dyes.

    Science.gov (United States)

    Rostami-Vartooni, Akbar; Nasrollahzadeh, Mahmoud; Alizadeh, Mohammad

    2016-05-15

    In the present work, silver nanoparticles were immobilized on the surface of seashell using Bunium persicum seeds extract as a reducing and stabilizing agent. The seashell supported silver nanoparticles was characterized by FT-IR spectroscopy, X-ray diffraction (XRD) pattern, field emission scanning electron microscopy (FE-SEM), energy dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM) and Thermogravimetric-differential thermal analysis (TG-DTA). The diameter of Ag NPs on the seashell was predominantly found within the range 20-50nm. The catalytic performance of the prepared catalyst was investigated for reduction of 4-Nitrophenol (4-NP), Methyl Orange (MO), Congo Red (CR) and Methylene Blue (MB) at room temperature. It was found that the Ag NPs/seashell is a highly active and recyclable catalyst for related reactions and can be recovered and reused several times without significant loss of its catalytic activity. Copyright © 2016 Elsevier Inc. All rights reserved.

  14. Application of dissolved air flotation on separation of waste plastics ABS and PS.

    Science.gov (United States)

    Wang, Hui; Chen, Xiao-Lei; Bai, Yang; Guo, Chao; Zhang, Li

    2012-07-01

    The aim of this research was to separate waste plastics acrylonitrile butadiene styrene (ABS) and polystyrene (PS) by dissolved air flotation in a self-designed dissolved air flotation apparatus. The effects of wetting agents, frother, conditioning time and flotation time on flotation behavior of waste plastics ABS (w-ABS) and PS (w-PS) were investigated and the optimized separation conditions were obtained. The results showed that when using 25 mgL(-1) tannic acid, 5 mgL(-1) terpineol, 15 min conditioning time and 15 min flotation time, mixtures of w-ABS and w-PS were separated successfully by dissolved air flotation in two stages, the results revealed that the purity and recovery rate of w-PS in the floated products were 90.12% and 97.45%, respectively, and the purity and recovery rate of w-ABS in the depressed products were 97.24% and 89.38%, respectively. Based on the studies of wetting mechanism of plastic flotation, it is found that the electrostatic force and hydrophobic attraction cannot be the main factor of the interaction between wetting agent molecules and plastic particles, which can be completed through water molecules as a mesophase, and a hydrogen bonding adsorption model with hydration shell as a mesophase was proposed. Copyright © 2012 Elsevier Ltd. All rights reserved.

  15. Pegylated polystyrene particles as a model system for artificial cells

    NARCIS (Netherlands)

    Meng, Fenghua; Engbers, G.H.M.; Gessner, Andrea; Müller, Reiner H.; Feijen, Jan

    2004-01-01

    Pegylated polystyrene particles (PS-PEG) were prepared as a model system for artificial cells, by modification of carboxyl polystyrene particles (PS-COOH) with homo- and hetero-bifunctional polyethylene glycols (PEG, MW 1500, 3400, and 5000) containing an amino end group for immobilization and an

  16. Psühhodramaatikud annavad Pärnus eksami

    Index Scriptorium Estoniae

    2008-01-01

    29. maist kuni 1. juunini kestab Pärnus psühhodraama konverents "Geeniuste kohtumine", kus rahvusvahelise koolituse läbinud annavad eksami. Ruuda Palmquist on psühhodraama kui teadusharu rajajaid Eestis. Pärnus on kohal Rootsi Moreno Instituudi juhataja, psühhodraama lavastaja Marc Treadwell

  17. Fuzzy Logic Particle Tracking

    Science.gov (United States)

    2005-01-01

    A new all-electronic Particle Image Velocimetry technique that can efficiently map high speed gas flows has been developed in-house at the NASA Lewis Research Center. Particle Image Velocimetry is an optical technique for measuring the instantaneous two component velocity field across a planar region of a seeded flow field. A pulsed laser light sheet is used to illuminate the seed particles entrained in the flow field at two instances in time. One or more charged coupled device (CCD) cameras can be used to record the instantaneous positions of particles. Using the time between light sheet pulses and determining either the individual particle displacements or the average displacement of particles over a small subregion of the recorded image enables the calculation of the fluid velocity. Fuzzy logic minimizes the required operator intervention in identifying particles and computing velocity. Using two cameras that have the same view of the illumination plane yields two single exposure image frames. Two competing techniques that yield unambiguous velocity vector direction information have been widely used for reducing the single-exposure, multiple image frame data: (1) cross-correlation and (2) particle tracking. Correlation techniques yield averaged velocity estimates over subregions of the flow, whereas particle tracking techniques give individual particle velocity estimates. For the correlation technique, the correlation peak corresponding to the average displacement of particles across the subregion must be identified. Noise on the images and particle dropout result in misidentification of the true correlation peak. The subsequent velocity vector maps contain spurious vectors where the displacement peaks have been improperly identified. Typically these spurious vectors are replaced by a weighted average of the neighboring vectors, thereby decreasing the independence of the measurements. In this work, fuzzy logic techniques are used to determine the true

  18. Psychometric properties of the French translation of the reduced KOOS and HOOS (KOOS-PS and HOOS-PS)

    DEFF Research Database (Denmark)

    Ornetti, P; Perruccio, A V; Roos, E M

    2009-01-01

    OBJECTIVE: To evaluate the psychometric properties of the French KOOS physical function (KOOS-PS) and HOOS physical function (HOOS-PS), specifically its feasibility, reliability, construct validity, and responsiveness. METHODS: Consecutive outpatients consulting for primary knee or hip osteoarthr......OBJECTIVE: To evaluate the psychometric properties of the French KOOS physical function (KOOS-PS) and HOOS physical function (HOOS-PS), specifically its feasibility, reliability, construct validity, and responsiveness. METHODS: Consecutive outpatients consulting for primary knee or hip...

  19. The influence of particle size and static magnetic fields on the uptake of magnetic nanoparticles into three dimensional cell-seeded collagen gel cultures.

    Science.gov (United States)

    Lewis, Emily E L; Child, Hannah W; Hursthouse, Andrew; Stirling, David; McCully, Mark; Paterson, David; Mullin, Margaret; Berry, Catherine C

    2015-08-01

    Over recent decades there has been and continues to be major advances in the imaging, diagnosis and potential treatment of medical conditions, by the use of magnetic nanoparticles. However, to date the majority of cell delivery studies employ a traditional 2D monolayer culture. This article aims to determine the ability of various sized magnetic nanoparticles to penetrate and travel through a cell seeded collagen gel model, in the presence or absence of a magnetic field. Three different sized (100, 200, and 500 nm) nanoparticles were employed in the study. The results showed cell viability was unaffected by the presence of nanoparticles over a 24-h test period. The initial uptake of the 100 nm nanoparticle into the collagen gel structure was superior compared to the larger sized nanoparticles under the influence of a magnetic field and incubated for 24 h. Interestingly, it was the 200 nm nanoparticles, which proved to penetrate the gel furthest, under the influence of a magnetic field, during the initial culture stage after 1-h incubation. © The Authors. Journal of Biomedical Materials Research Part B: Applied Biomaterials Published by Wiley Periodicals, Inc.

  20. Evolving science enhanced with iPS

    Directory of Open Access Journals (Sweden)

    Editor

    2007-11-01

    Full Text Available Dear friends, Greetings from all in the team. With the stage set for online submissions and the review-response-revision-resubmission process standardized, we have come with the first regular issue and from now there will be quarterly issues of the journal. Since the starting of the JSRM in a short span there have been a lot of developments, which we would rather say as "evolutions" keeping in mind, the recent iPS! This evolution we would like you to see from a background of the various developments in the art and science of medicine throughout in the past three centuries. We have come across the era of investigative tools such as bamboo made laryngoscopes to era of vaccines and antibiotics followed by the era of revolutionary non-invasive procedures and recently the nano technology based drugs and now the iPS! Macro to Micro, but still more to go. All through the influence of the society, religions, philosophies have been playing a very important role in every step the science of biology moves ahead. Starting with the contraception, assisted reproduction then the gene modified plants....and now the embryonic stem cells! With the advent of the iPS, though the issues of oncogenes, teratoma yet to be ruled out, we have found there is a way which can bypass the ES cells! Hats off to those scientists who have burnt their midnight oil to have found this way out! The lesson we learn is to explore things with an open mind and continue to proceed further without spending much time fingers crossed. Yours sincerely,The Editorial team.

  1. Presence of retained crystalline seed necessary for bicrystal-liquid-bicrystal phase transformation

    Science.gov (United States)

    Vijay Reddy, K.; Meraj, Md.; Pal, Snehanshu

    2017-10-01

    Regaining of the original bicrystal structure during solidification after melting is found to be possible only if a fraction of crystalline seed is retained in the melt as per this molecular dynamics study. The effect of cooling rate on retaining the crystalline seed is also investigated. The bicrystal specimen is heated up to 1850 K with a constant heating rate of 3.5 K ps-1 and instantly cooled with different cooling rates i.e. 1 K ps-1, 5 K ps-1, 10 K ps-1, 15 K ps-1 and 20 K ps-1. It has been observed through total energy analysis that specimen cooled with 1 K ps-1 cooling rate dissipates the energy throughout the specimen causing randomization of atoms resulting into disappearance of the crystalline seed and formation of nanocrystalline structure. Whereas comparatively higher cooling rates does not allow complete dissipation of energy and retain the crystalline seed thus transform into bicrystal. It has been found through Voronoi polyhedra analysis that during slower cooling rate, icosahedral clusters are formed which play a vital role in formation of nanocrystalline structure.

  2. Position pickup of the PS Booster

    CERN Multimedia

    CERN PhotoLab

    1975-01-01

    The beam position around the 4 rings of the PS Booster (originally 800 MeV, now 1.4 GeV), is measured with electrostatic pickups (PU). They consist of a ceramic cylinder forming part of the vacuum chamber, and, in order to save space, they are located inside the multipole lenses. The inside of the ceramic is coated with a metallic layer, into which the form of the electrodes was cut by computer-controlled micro-sandblasting. Each PU has a pair of horizontal and a pair of vertical electrodes, as well as a separate intensity-sensing circular electrode.

  3. Space charge studies in the PS

    CERN Document Server

    Asvesta, F; Damerau, H; Huschauer, A; Papaphilippou, Y; Serluca, M; Sterbini, G; Zisopoulos, P

    2017-01-01

    In this paper the results of Machine Development (MD)studies conducted at the CERN Proton Sychrotron (PS) arepresented. The main focus was the investigation of newworking points in an effort to characterize and potentiallyimprove the brightness for LHC-type beams in view of theLHC Injectors Upgrade (LIU). Various working points werecompared in terms of losses and emittance evolution. Sincespace charge and the resonances it excites are the main causefor emittance blow-up and losses, tunes close to excitedresonances were carefully studied. Mitigation techniques,such as bunch flattening using a double harmonic RF system,were also tested.

  4. PAN/PS elctrospun fibers for oil spill cleanup

    Science.gov (United States)

    Ying, Qiao; Lili, Zhao; Haixiang, Sun; Peng, Li

    2014-08-01

    A high-capacity oil sorbent was fabricated by electrospinning using PS/PAN blend. Morphology, contact angle and oil adsorption of PAN/PS fiber and PP nonwoven fabric were studied. It was found that the PAN/PS fiber had a smaller diameter than PP, and the maximum sorption capacities of the PAN/PS sorbent for pump oil, peanut oil, diesel, and gasoline were 194.85, 131.7, 66.75, and 43.38 g/g, which were far higher than those of PP. The sorbent PS/PAN fiber showed a contact angle of water144.32° and diesel oil 0°. The sorption kinetics of PAN/PS and PP sorbent were also investigated. Compared with the commercial PP fabric, the PAN/PS fiber seems to have the ability to be used in oil-spill cleanup application.

  5. Chemotherapy and quality of life in NSCLC PS 2 patients

    DEFF Research Database (Denmark)

    Helbekkmo, Nina; Strøm, Hans H; Sundstrøm, Stein H

    2009-01-01

    INTRODUCTION: Nearly 40% of patients with advanced NSCLC are in performance status (PS) 2. These patients have a shorter life expectancy than PS 0/1 patients and they are underrepresented in clinical trials. Data on how platinum-based combination chemotherapy affects Health Related Quality of Life...... (HRQOL) of patients with PS 2 are scarce and the treatment of this important group of patients is controversial. METHODS: A national multicenter phase III study on platinum based chemotherapy to 432 advanced NSCLC patients included 123 patients with PS 2. To explore the treatment impact on HRQOL......: Whereas the demographic data at baseline were well balanced between the groups, the PS 2 patients had significantly worse function and more severe symptoms than the PS 0/1 patients. In response to combination chemotherapy, the PS 2 patients had a more profound improvement of global QOL, cognitive function...

  6. Sofrimento psíquico e trabalho

    Directory of Open Access Journals (Sweden)

    Sarah Rosa Salles Vieira

    2014-03-01

    Full Text Available O presente artigo aprofunda questões clínico-téoricas relacionadas especificamente ao trabalho docente e ao sofrimento psíquico a ele relacionado a partir da observação clínica e vivência grupal nos atendimentos terapêuticos ocupacionais realizados no Hospital do Servidor Público Estadual de São Paulo "Francisco Morato de Oliveira" (HSPE-FMO. Partindo dos estudos acerca da Psicopatologia do Trabalho de Christophe Dejours, do trabalho docente e do relato de um caso clínico, caracteriza a problemática do sofrimento no trabalho, os sistemas de defesa contra este sofrimento, a ameaça à subjetividade do próprio trabalhador, as representações e conflitos vivenciados no trabalho docente, bem como a relação aditiva estabelecida como uma estratégia inconsciente de sobrevivência psíquica.

  7. KAJIAN INDUCED PLURIPOTENT STEMCELL (iPS (HARAPAN DAN TANTANGAN

    Directory of Open Access Journals (Sweden)

    Masagus Zainuri

    2014-05-01

    Full Text Available AbstractInduced Pluripotent Stemcell (iPS are adult cells which the genetic information in the nucleus of those cells being reprogrammed (reprogram by inserting exogenous pluripotential genes. The exogenous gene transduction is using vectors, such as lentivirus, retrovirus, or adenovirus, which suppressed the gene expression of the original cells, so they will express the transduced exogenous gene. Viral vectors are then used to reprogramming and producing iPS clones that are pluripotent. iPS derived from adult cells of patient with certain diseases will be used as a tool to study the mechanisms of those specific diseases and the effects of selected drugs against the diseases. Several previous studies have shown that iPS clones developed from specific genetic disease have its original genotype and retain the character of the response to the drug that similar as the original adult cells. Opportunities for the utilization of autologous iPS cell therapy in the future is wide open as expected iPS transplant will not be rejected when transplanted back to the patient. Behind all its potential, iPS production is still facing some problems to be applicable clinically. The use of viruses as vectors may cause problems due to virus gene sequences may be integrated into the genome of the DNA donor cell, thereby causing mutations of the iPS clones. Several subsequent studies have succeeded in replacing the use of viruses as vectors, but the level of efficiency obtained is still very low. Another problem that arises is that epigenetic changes may occur in iPS cultures. Many advanced research related to iPS may be developed in Indonesia and is necessary to improve the production efficiency of iPS and solve iPS clones epigenetic changes problems in the future.Keywords: iPS, pluripotency, transduction, transfection.AbstrakInduced Pluripotent Stemcell (iPS adalah sel somatic dewasa yang informasi genetika dalam inti selnyadiprogram ulang (reprogram dengan cara

  8. Comparison of molecular species of various transphosphatidylated phosphatidylserine (PS) with bovine cortex PS by mass spectrometry

    NARCIS (Netherlands)

    Chen, S.; Li, K.W.

    2008-01-01

    The exogenous introduction of a molecular species mixture of bovine cortex phosphatidylserine (BC-PS) has been claimed to improve memory function in subjects suffering from age-associated memory impairment and dementia. However, it has been also reported that oral administration of another molecular

  9. bFGF and Activin A function to promote survival and proliferation of single iPS cells in conditioned half-exchange mTeSR1 medium.

    Science.gov (United States)

    Guo, Xiaoling; Lian, Ruiling; Guo, Yonglong; Liu, Qing; Ji, Qingshan; Chen, Jiansu

    2015-07-01

    Human induced pluripotent stem (iPS) cells can be well maintained by clonal growth. The pluripotent growth of single iPS cells is limited by low survival. To facilitate robust single iPS cells cultured in vitro, half-exchange mTeSR1 medium (HM), whole-exchange medium (WM) and iPS cell-derived conditioned medium (iPS-CM) culture were used. The effects of bFGF and Activin A on the growth of single iPS cells were explored. The dissociation and propagation of single iPS cells also included Accutase enzymatic isolation, Rho-associated protein kinase (ROCK) inhibitor Y27632 protection and high-density single-cell seeding (1 × 10(6) cells/well). CCK-8 assays demonstrated that the viability of clonal iPS cells in mTeSR1 medium and single iPS cells in HM, iPS-CM or WM supplemented with 100 ng/ml bFGF and 10 ng/ml Activin A was significantly higher than that in WM. Annexin v and propidium iodide (PI) assay, Calcein AM and EthD-III double staining also confirmed the similar results. ELISA assays showed that the levels of bFGF and Activin A of single iPS cells in HM and iPS-CM were higher than single iPS cells in WM. Meanwhile, Reverse Transcription-Polymerase Chain Reaction (RT-PCR), quantitative Polymerase Chain Reaction (qPCR), Western Blotting (WB), Immunofluorescence (IF) and karyotype analysis revealed that HM culture was able to maintain undifferentiated markers of Nanog, Klf4, Sox2, Oct4, and did not affect the karyotype of iPS cells. Undifferentiated single iPS cells in HM displayed homogenized growth. These findings demonstrate that bFGF and Activin A are important for the survival and growth of single iPS cells. HM culture system combined Accutase, Y27632 and high-density single-cell seeding can facilitate short-term growth of single iPS cells in vitro.

  10. PS main supply: motor-generator set.

    CERN Multimedia

    Maximilien Brice

    2002-01-01

    In picture 04 the motor is on the right in the background and the main view is of the generator. The peak power in each PS cycle drawn from the generator, up to 96 MW, is taken from the rotational kinetic energy of the rotor (a heavy-weight of 80 tons), which makes the rotational speed drop by only a few percent. The motor replenishes the average power of 2 to 4 MW. Photo 05: The motor-generator set is serviced every year and, in particular, bearings and slip-rings are carefully checked. To the left is the motor with its slip-rings visible. It has been detached from the axle and moved to the side, so that the rotor can be removed from the huge generator, looming at the right.

  11. Beam test results of a 16 ps timing system based on ultra-fast silicon detectors

    Science.gov (United States)

    Cartiglia, N.; Staiano, A.; Sola, V.; Arcidiacono, R.; Cirio, R.; Cenna, F.; Ferrero, M.; Monaco, V.; Mulargia, R.; Obertino, M.; Ravera, F.; Sacchi, R.; Bellora, A.; Durando, S.; Mandurrino, M.; Minafra, N.; Fadeyev, V.; Freeman, P.; Galloway, Z.; Gkougkousis, E.; Grabas, H.; Gruey, B.; Labitan, C. A.; Losakul, R.; Luce, Z.; McKinney-Martinez, F.; Sadrozinski, H. F.-W.; Seiden, A.; Spencer, E.; Wilder, M.; Woods, N.; Zatserklyaniy, A.; Pellegrini, G.; Hidalgo, S.; Carulla, M.; Flores, D.; Merlos, A.; Quirion, D.; Cindro, V.; Kramberger, G.; Mandić, I.; Mikuž, M.; Zavrtanik, M.

    2017-04-01

    In this paper we report on the timing resolution obtained in a beam test with pions of 180 GeV/c momentum at CERN for the first production of 45 μm thick Ultra-Fast Silicon Detectors (UFSD). UFSD are based on the Low-Gain Avalanche Detector (LGAD) design, employing n-on-p silicon sensors with internal charge multiplication due to the presence of a thin, low-resistivity diffusion layer below the junction. The UFSD used in this test had a pad area of 1.7 mm2. The gain was measured to vary between 5 and 70 depending on the sensor bias voltage. The experimental setup included three UFSD and a fast trigger consisting of a quartz bar readout by a SiPM. The timing resolution was determined by doing Gaussian fits to the time-of-flight of the particles between one or more UFSD and the trigger counter. For a single UFSD the resolution was measured to be 34 ps for a bias voltage of 200 V, and 27 ps for a bias voltage of 230 V. For the combination of 3 UFSD the timing resolution was 20 ps for a bias voltage of 200 V, and 16 ps for a bias voltage of 230 V.

  12. Beam test results of a 16 ps timing system based on ultra-fast silicon detectors

    Energy Technology Data Exchange (ETDEWEB)

    Cartiglia, N., E-mail: cartiglia@to.infn.it [INFN, Torino (Italy); Staiano, A.; Sola, V. [INFN, Torino (Italy); Arcidiacono, R. [INFN, Torino (Italy); Università del Piemonte Orientale (Italy); Cirio, R.; Cenna, F.; Ferrero, M.; Monaco, V.; Mulargia, R.; Obertino, M.; Ravera, F.; Sacchi, R. [INFN, Torino (Italy); Università di Torino, Torino (Italy); Bellora, A.; Durando, S. [Università di Torino, Torino (Italy); Mandurrino, M. [Politecnico di Torino, Torino (Italy); Minafra, N. [University of Kansas, KS (United States); Fadeyev, V.; Freeman, P.; Galloway, Z.; Gkougkousis, E. [SCIPP, University of California Santa Cruz, CA 95064 (United States); and others

    2017-04-01

    In this paper we report on the timing resolution obtained in a beam test with pions of 180 GeV/c momentum at CERN for the first production of 45 µm thick Ultra-Fast Silicon Detectors (UFSD). UFSD are based on the Low-Gain Avalanche Detector (LGAD) design, employing n-on-p silicon sensors with internal charge multiplication due to the presence of a thin, low-resistivity diffusion layer below the junction. The UFSD used in this test had a pad area of 1.7 mm{sup 2}. The gain was measured to vary between 5 and 70 depending on the sensor bias voltage. The experimental setup included three UFSD and a fast trigger consisting of a quartz bar readout by a SiPM. The timing resolution was determined by doing Gaussian fits to the time-of-flight of the particles between one or more UFSD and the trigger counter. For a single UFSD the resolution was measured to be 34 ps for a bias voltage of 200 V, and 27 ps for a bias voltage of 230 V. For the combination of 3 UFSD the timing resolution was 20 ps for a bias voltage of 200 V, and 16 ps for a bias voltage of 230 V.

  13. Nuclear characterization of radioactive bioglass seed for brachytherapy studies

    Energy Technology Data Exchange (ETDEWEB)

    Nogueira, L.B.; Campos, T.P.R., E-mail: Lucibn19@yahoo.com.br, E-mail: Campos@nuclear.ufmg.br [Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG (Brazil). Departamento de Engenharia Nuclear

    2011-07-01

    This paper aims to analyze the isotopic compositions of the radioactive bioglass seeds for brachytherapy studies. Bioglass seeds were synthesized by sol-gel process, distributed in the seed samples, such as [Si: Ca: Ho], [Si: Ca: Ho: Zr], [Si: Ca: Ho: Ba], [Si: Ca: Sm] and [Si: Ca: Sm: Ba]. The bioglass seeds were irradiated at the TRIGA nuclear reactor - CDTN for a period of eight hours on a turntable around the reactor core for nuclear characterization studies. Nuclear characterization of the radioactive bioglass seeds by gamma spectrometry provided the gamma signatures of Sm-153 and Ho-166 followed by the Ba and Zr contrast agents. The gamma and X-ray spectra were plotted for analysis of the isotopic compositions of bioglass seeds. Gamma spectrum from the Ho-166 radioisotope and the X-ray spectrum of the Ba and Zr elements for [Si: Ca: Ho: Ba] and [Si: Ca: Ho: Zr] were showed. The particle ranges on ceramic; water and tissue were also analyzed using gamma and beta particle evaluations. The beta particle is absorbed around the seed, whereas the gamma particle can travel far from the seed position. Therefore, for small volume and consequentially small mass, the absorbed dose of beta particles close to the seed is very high. These results complement the study of the characterization and monitoring of bioglass seeds for brachytherapy implants. (author)

  14. Electron microscopic study on aerosol-assisted synthesis of aluminum organophosphonates using flexible colloidal PS-b-PEO templates.

    Science.gov (United States)

    Kimura, Tatsuo; Yamauchi, Yusuke

    2012-09-04

    A wide variety of synthetic approaches from homogeneous precursor solutions have so far been developed for precise structural design of materials in multiscale. In organic templating approaches for porous materials design, we have recently developed a new approach to fabricate colloidal polystyrene-block-poly(oxyethylene) (PS-b-PEO) templated large pores that can be controlled in thick films of aluminum organophosphonate (AOP). In this study, we extended this approach using colloidal PS-b-PEO aggregates to aerosol-assisted synthesis for the fabrication of spherical particles. Structural variations (morphology and porous structure) depended on the synthetic conditions, which were mainly investigated by using electron microscopies (SEM and TEM). In addition to the insight on the colloidal PS-b-PEO templating of spherical pores in AOP spheres, it was found that colloidal PS-b-PEO aggregates were flexible for further design of pore shape that was strongly affected by external morphology. In this context, we proposed this method as flexible colloidal PS-b-PEO templating to fabricate unusual macroporous structures during morphological control from precursor solutions containing colloidal PS-b-PEO aggregates. The insights will be promising for precise construction of unique devices using porous materials templated by colloidal organic aggregates. In addition, we found a useful water adsorption-desorption behavior over the macroporous AOP bulky powders when the macropores were connected through large pores, which is also significant for future development of AOP-based porous materials.

  15. PS: A nonprocedural language with data types and modules

    Science.gov (United States)

    Gokhale, M. B.

    1986-01-01

    The Problem Specification (PS) nonprocedural language is a very high level language for algorithm specification. PS is suitable for nonprogrammers, who can specify a problem using mathematically-oriented equations; for expert programmers, who can prototype different versions of a software system for evaluation; and for those who wish to use specifications for portions (if not all) of a program. PS has data types and modules similar to Modula-2. The compiler generates C code. PS is first shown by example, and then efficiency issues in scheduling and code generation are discussed.

  16. Distinct iPS Cells Show Different Cardiac Differentiation Efficiency.

    Science.gov (United States)

    Ohno, Yohei; Yuasa, Shinsuke; Egashira, Toru; Seki, Tomohisa; Hashimoto, Hisayuki; Tohyama, Shugo; Saito, Yuki; Kunitomi, Akira; Shimoji, Kenichiro; Onizuka, Takeshi; Kageyama, Toshimi; Yae, Kojiro; Tanaka, Tomofumi; Kaneda, Ruri; Hattori, Fumiyuki; Murata, Mitsushige; Kimura, Kensuke; Fukuda, Keiichi

    2013-01-01

    Patient-specific induced pluripotent stem (iPS) cells can be generated by introducing transcription factors that are highly expressed in embryonic stem (ES) cells into somatic cells. This opens up new possibilities for cell transplantation-based regenerative medicine by overcoming the ethical issues and immunological problems associated with ES cells. Despite the development of various methods for the generation of iPS cells that have resulted in increased efficiency, safety, and general versatility, it remains unknown which types of iPS cells are suitable for clinical use. Therefore, the aims of the present study were to assess (1) the differentiation potential, time course, and efficiency of different types of iPS cell lines to differentiate into cardiomyocytes in vitro and (2) the properties of the iPS cell-derived cardiomyocytes. We found that high-quality iPS cells exhibited better cardiomyocyte differentiation in terms of the time course and efficiency of differentiation than low-quality iPS cells, which hardly ever differentiated into cardiomyocytes. Because of the different properties of the various iPS cell lines such as cardiac differentiation efficiency and potential safety hazards, newly established iPS cell lines must be characterized prior to their use in cardiac regenerative medicine.

  17. Burkholderia phytofirmans PsJN reduces damages to freezing temperature in Arabidopsis thaliana

    Directory of Open Access Journals (Sweden)

    Fan eSU

    2015-10-01

    Full Text Available Several plant growth-promoting rhizobacteria (PGPR are known to improve plant tolerance to multiple stresses, including low temperatures. However, mechanisms underlying this protection are still poorly understood. The aim of this study was to evaluate the role of the endophytic PGPR, Burkholderia phytofirmans strain PsJN (Bp PsJN, on Arabidopsis thaliana cold tolerance using photosynthesis parameters as physiological markers.Under standard conditions, our results indicated that Bp PsJN inoculation led to growth promotion of Arabidopsis plants without significant modification on photosynthesis parameters and chloroplast organization. However, bacterial colonization induced a cell wall strengthening in the mesophyllImpact of inoculation modes (either on seeds or by soil irrigation and their effects overnight at 0, -1 or -3°C, were investigated by following photosystem II (PSII activity and gas exchanges. Following low temperatures stress, a decrease of photosynthesis parameters was observed. In addition, during three consecutive nights or days at -1°C, PSII activity was monitored. Pigment contents, RuBisCO protein abundance, expression of several genes including RbcS, RbcL, CBF1, CBF2, CBF3, ICE1, COR15a, and COR78 were evaluated at the end of exposure. To assess the impact of the bacteria on cell ultrastructure under low temperatures, microscopic observations were achieved. Results indicated that freezing treatment induced significant changes in PSII activity as early as the first cold day, whereas the same impact on PSII activity was observed only during the third cold night. The significant effects conferred by PsJN were differential accumulation of pigments, and reduced expression of RbcL and COR78. Microscopical observations showed an alteration/disorganization in A. thaliana leaf mesophyll cells independently of the freezing treatments. The presence of bacteria during the three successive nights or days did not significantly improved A

  18. Water Absorption Behavior of Polystyrene Particles Prepared by Emulsion Polymerization with Nonionic Emulsifiers and Innovative Easy Synthesis of Hollow Particles.

    Science.gov (United States)

    Okubo, Masayoshi; Kobayashi, Hiroshi; Huang, Chujuan; Miyanaga, Eri; Suzuki, Toyoko

    2017-04-11

    Submicrometer-sized raspberry-like polystyrene (PS) particles, which were prepared by emulsion polymerization with polyoxyethylene nonylphenyl ether nonionic emulsifier (Emulgen 910, HLB 12.2) and potassium persulfate initiator, contained 8.5 vol % (relative to the particle) of water and 5.5 wt % (relative to PS) of Emulgen 910 in the inside. The water absorption decreased the glass transition temperature of the PS particles dispersed in an aqueous medium. The wt % (relative to PS) of the incorporated Emulgen 910 increased with increasing initial Emulgen 910 concentration in the emulsion polymerization, but the wt % (relative to the total Emulgen 910 used) of the incorporated Emulgen 910 was constant at approximately 50% independent of the initial concentration. The vol % (relative to particle) of water increased to 46% by heat treatment at 90 °C for 24 h, which was based on further water absorption, and resulted in spherical hollow particles, where the amount of the incorporated Emulgen 910 remarkably decreased in a short treatment and then remained almost constant during the heat treatment. After another 24 h treatment, the percentage of nonhollow particles increased gradually, which was based on the escape of the water domain together with Emulgen 910 from the inside of the particles. On the other hand, spherical PS particles prepared by emulsifier-free emulsion polymerization did not contain water in the inside and were not changed to hollow ones by a similar heat treatment. From these results, an innovative easy method to synthesize hydrophobic hollow PS particles is proposed.

  19. LEADIR-PS: providing unprecedented SMR safety and economics

    Energy Technology Data Exchange (ETDEWEB)

    Hart, R.S., E-mail: N2i2@xplornet.ca [Northern Nuclear Industries Incorporated, Cambridge, ON (Canada)

    2015-07-01

    Northern Nuclear Industries Incorporated (N{sup 2} I{sup 2}) is developing Small Modular Reactors (SMRs) called LEADIR-PS, an acronym for LEAD-cooled Integral Reactor-Passively Safe. LEADIR-PS integrates proven technologies including TRISO fuel, Pebble Bed core and graphite moderator, with molten lead coolant in an integral pool type reactor configuration to achieve unprecedented safety and economics. Plants under development are LEADIR-PS30, producing 30 MWth, LEADIR-PS100 producing 100 MWth and LEADIR-PS300 producing 300 MWth that are focused on serving the energy demands of areas with a small electrical grid and/or process heat applications. A plant consisting of six LEADIR-PS300 reactor modules serving a common turbine-generator, called the LEADIR-PS Six-Pack, is focused on serving areas with higher energy demands and a robust electricity grid. The Gen{sup +} I LEADIR-PS plants are inherently/passively safe. There is no potential for a Loss Of Coolant Accident, a reactivity transient without shutdown, a loss of heat sink, or hydrogen generation. No active systems or operator actions are required to assure safety. The unprecedented safety of LEADIR-PS reactors avoids large exclusion radius and demanding evacuation plan requirements. LEADIR-PS, with steam conditions of 370 {sup o}C and 12 MPa can serve over 85% of the world's non-transportation process heat demands. In Canada, the electricity and process heat demands, ranging from those of remote communities and the oil sands to densely populated areas can be served by LEADIR-PS. (author)

  20. Recent improvements of the RF Beam control for LHC-type beams in the CERN PS

    CERN Document Server

    Damerau, H; Schokker, M

    2010-01-01

    To cope with the large variety of different beams for the LHC, the RF beam control in the CERN PS has evolved continuously to improve its flexibility and reliability. Single-bunch beams, several different multi-bunch beams with 25, 50 or 75 ns bunch spacing at ejection for LHC filling, as well as two lead-ion beam variants are now regularly produced in pulse-to-pulse operation. The multibunch beam control for protons can be easily re-adjusted from 0.25 · 1011 to 1.3 · 1011 particles per ejected bunch. Depending on the number of bunches injected from the PS Booster, the length of the ejected bunch train may vary from 8 to 72 bunches. This paper summarizes recent improvements in the low-level RF systems and gives an outlook on the future consolidation.

  1. Effects of Humidity On the Flow Characteristics of PS304 Plasma Spray Feedstock Powder Blend

    Science.gov (United States)

    Stanford, Malcolm K.; DellaCorte, Christopher

    2002-01-01

    The effects of environmental humidity on the flow characteristics of PS304 feedstock have been investigated. Angular and spherical BaF2-CaF2 powder was fabricated by comminution and by atomization, respectively. The fluorides were added incrementally to the nichrome, chromia, and silver powders to produce PS304 feedstock. The powders were dried in a vacuum oven and cooled to a Tom temperature under dry nitrogen. The flow of the powder was studied from 2 to 100 percent relative humidity (RH) The results suggest that the feedstock flow is slightly degraded with increasing humidity below 66 percent RH and is more affected above 66 percent RH. There was no flow above 88 percent RH. Narrower particle size distributions of the angular fluorides allowed flow up to 95 percent RH. These results offer guidance that enhances the commercial potential for this material system.

  2. Synthesis of poly (methyl methacrylate)-b-polystyrene with high molecular weight by DPE seeded emulsion polymerization and its application in proton exchange membrane.

    Science.gov (United States)

    Wang, Wenwen; Zhang, Hepeng; Geng, Wangchang; Gu, Junwei; Zhou, Yanyang; Zhang, Junping; Zhang, Qiuyu

    2013-09-15

    In this article, we present poly (methyl methacrylate)-b-polystyrene (PMMA-b-PS) with different block ratios and high molecular weight, which was synthesized by environmentally friendly seeded emulsion polymerization with 1,1-diphenylethylene (DPE) as a chain transfer agent. Polymerization kinetics in the first and second stage was investigated. Stable latex and homogeneous latex particles were obtained with the characterization of laser light scattering (LLS) and transmission electron microscopy (TEM). SEC and (1)H NMR revealed the successful preparation of block copolymers with high molecular weight and two different block ratios. The morphology of microphase separation of block copolymer thin films was investigated by AFM, and long-range order lamellar morphology was observed after vapor-annealing. The block copolymer with block ratio of almost 1:1 and higher molecular weight than that of previous PMMA-b-PS was sulfonated with acetyl sulfate, and the sulfonation was confirmed by FTIR, (1)H NMR, and TGA. Then, the sulfonated PMMA-b-PS was casted as membranes. The electrochemical impedance spectroscopy displayed that membranes possessed favorable proton conductivity and fine dimensional stability, and they could be candidates as proton exchange membranes. Copyright © 2013 Elsevier Inc. All rights reserved.

  3. Interleaving of beam lines inside the PS tunnel

    CERN Multimedia

    1983-01-01

    View against the direction of the proton beams. The PS ring (section 26) is on the left. The injection tunnel for LEAR leaving from here has increased the trafic in this already busy area where the two Linacs and the transfer tunnel leading to the SPS, ISR and AA join the PS ring (cf. photo 7802260, 7802261, Annual Report 1981, p. 89, fig. 12).

  4. Modulation of enzymatic PS synthesis by liposome membrane composition.

    Science.gov (United States)

    Pinsolle, Alexandre; Roy, Philippe; Cansell, Maud

    2014-03-01

    Phosphatidylserine (PS) is a phospholipid known to exert important physiological roles in humans. However, this phospholipid (PL) is poorly available as a natural source and hardly produced by the chemical route. In this work, PS was obtained by transphosphatidylation using phospholipase D (PLD) and PL self-assembled into liposomes as the substrates. The aim was to better understand how the liposome membrane composition could modulate PS yield. Three lecithins were used as PL substrates, one originated from a marine source providing a high amount of n-3 polyunsaturated fatty acids, and two issued from soya differing in their phosphatidylcholine (PC) content. Different parameters such as Ca(2+) content, enzyme and L-serine concentrations modulated PS synthesis. The presence of Ca(2+) increased PS conversion yield. The alcohol acceptor (L-serine) concentration positively acted on PL conversion, by governing the equilibrium between transphosphatidylation and hydrolysis. Beside these specific reaction conditions, it was demonstrated that the membrane composition of the liposomes modulated PS synthesis. A direct correlation between PS accumulation and the amount of cholesterol or α-tocopherol incorporated into the soya lecithins was observed. This result was interpreted in terms of "head" spacers promoting PLD transphosphatidylation. On the whole, this work provided key parameters for the formulation of liposomes using enzymatic PLD technology, to produce lecithins enriched in different proportions of PS and esterified with various types of fatty acids depending on the initial lecithin source. Copyright © 2013 Elsevier B.V. All rights reserved.

  5. Spectroscopic Classification of PS16chs with SOAR/Goodman

    Science.gov (United States)

    Miller, J. A.; Hounsell, R. A.; Pan, Y.-C.; Foley, R. J.; Jha, S. W.; Rest, A.; Scolnic, D.

    2016-05-01

    We report the classification of PS16chs from spectroscopic observation with the Goodman spectrograph on the SOAR telescope. The observation was made on 2016 May 08 UT. We classify PS16chs as a SN Ia near maximum light at z = 0.19.

  6. Motor-Generator powering the PS (Proton Synchrotron) main magnets

    CERN Multimedia

    1983-01-01

    This motor-generator,30 MW peak, 1500 r.p.m.,pulsed power supply for the PS main magnet replaced in 1968 the initial 3000 r.p.m. motor-generator-flywheel set which had served from the PS start-up in 1959 until end 1967. See also photo 8302337 and its abstract.

  7. Psühhodraama - spontaansuse kool / Taimi Elenurm

    Index Scriptorium Estoniae

    Elenurm, Taimi

    2010-01-01

    Viinis ja New Yorgis tegutsenud psühhiaatri Jakob Levy Moreno loodud psühhodraamast, mis võimaldab rollimängu kaudu näha ennast läbi teiste silmade, aga ka vabaneda pingetest andes võimaluse käituda teisiti kui tavaelus

  8. on oil palm seeds

    African Journals Online (AJOL)

    User

    flies' activities caused damage to seeds including rotten kennels and seeds, empty shells and dead developing embryos in transparent polyethylene storage bags. The highest infestation was on 2052 seeds out of a total production of 582,503 germinated seeds in batch number 5 and the lowest was 223 seeds out of ...

  9. seed oil

    African Journals Online (AJOL)

    Wara

    The foam height of the soap was found to be 2.0 cm which is lower than that of Jatropha oil soap(5.4cm), Sesame oil soap(4.8cm), Cotton seed oil soap(4.5cm) and shea nut soap(4.2cm),t higher than that of Castor oil soap(1.6cm) and Castor glycerine soap(1.4cm). The soap was milk in colour and slightly soluble in distilled ...

  10. Successful online learning – the five Ps

    Directory of Open Access Journals (Sweden)

    Jim FLOOD

    2004-04-01

    Full Text Available Successful online learning – the five Ps Jim FLOOD E-learning Consultant-UK jimflood@btinternet.com Key learning points • An important aspect of design for online learning is visual ergonomics. • Learning theories offer poor predictive power in terms of how learners work and learn. • Success at learning is closely related to emotional engagement–and learning designers tend to ignore this aspect. • Online learning poses a challenging experience for learners–and they need support to cope with it. • A key goal to achieve Praxis – being able to put learning into practice. Many of you will be familiar with the three (or more Ps of marketing and even if not, as trainers or teachers you are likely to have used mnemonics as an aid to retention and recall. Mnemonics are especially useful when you need to get the key points to ‘stick’ in the minds of your audience. With this in mind I offer you the 5 Ps of online learning: Presentation, Pedagogy, Promotion, Preparation and Props. What I offer is not new; in fact much of it results from the eleven years of online teaching and learning at The Open University, the £22 million it has spent on research and evaluation 1, and the worldwide community that have been sharing experience in recent years. You can therefore consider these 5 Ps to be a convenient re-packing of the information and experience that can be found in abundance on the Internet. Presentation Good graphic design appeals to the subtle process by which the brain processes information and, as a result, we decide if we like the ‘look and feel’ of a visual environment. Part of liking this ‘look and feel’ is the way the text and pictorial layout can appear inviting and encouraging–a vital aspect of any online learning environment. Another aspect of presentation is how the text reads in terms of engaging the learner and introducing the story to be told–as well as being written in clear and concise English When browsing through books

  11. Telomere reprogramming and maintenance in porcine iPS cells.

    Directory of Open Access Journals (Sweden)

    Guangzhen Ji

    Full Text Available Telomere reprogramming and silencing of exogenous genes have been demonstrated in mouse and human induced pluripotent stem cells (iPS cells. Pigs have the potential to provide xenotransplant for humans, and to model and test human diseases. We investigated the telomere length and maintenance in porcine iPS cells generated and cultured under various conditions. Telomere lengths vary among different porcine iPS cell lines, some with telomere elongation and maintenance, and others telomere shortening. Porcine iPS cells with sufficient telomere length maintenance show the ability to differentiate in vivo by teratoma formation test. IPS cells with short or dysfunctional telomeres exhibit reduced ability to form teratomas. Moreover, insufficient telomerase and incomplete telomere reprogramming and/or maintenance link to sustained activation of exogenous genes in porcine iPS cells. In contrast, porcine iPS cells with reduced expression of exogenous genes or partial exogene silencing exhibit insufficient activation of endogenous pluripotent genes and telomerase genes, accompanied by telomere shortening with increasing passages. Moreover, telomere doublets, telomere sister chromatid exchanges and t-circles that presumably are involved in telomere lengthening by recombination also are found in porcine iPS cells. These data suggest that both telomerase-dependent and telomerase-independent mechanisms are involved in telomere reprogramming during induction and passages of porcine iPS cells, but these are insufficient, resulting in increased telomere damage and shortening, and chromosomal instability. Active exogenes might compensate for insufficient activation of endogenous genes and incomplete telomere reprogramming and maintenance of porcine iPS cells. Further understanding of telomere reprogramming and maintenance may help improve the quality of porcine iPS cells.

  12. Telomere reprogramming and maintenance in porcine iPS cells.

    Science.gov (United States)

    Ji, Guangzhen; Ruan, Weimin; Liu, Kai; Wang, Fang; Sakellariou, Despoina; Chen, Jijun; Yang, Yang; Okuka, Maja; Han, Jianyong; Liu, Zhonghua; Lai, Liangxue; Gagos, Sarantis; Xiao, Lei; Deng, Hongkui; Li, Ning; Liu, Lin

    2013-01-01

    Telomere reprogramming and silencing of exogenous genes have been demonstrated in mouse and human induced pluripotent stem cells (iPS cells). Pigs have the potential to provide xenotransplant for humans, and to model and test human diseases. We investigated the telomere length and maintenance in porcine iPS cells generated and cultured under various conditions. Telomere lengths vary among different porcine iPS cell lines, some with telomere elongation and maintenance, and others telomere shortening. Porcine iPS cells with sufficient telomere length maintenance show the ability to differentiate in vivo by teratoma formation test. IPS cells with short or dysfunctional telomeres exhibit reduced ability to form teratomas. Moreover, insufficient telomerase and incomplete telomere reprogramming and/or maintenance link to sustained activation of exogenous genes in porcine iPS cells. In contrast, porcine iPS cells with reduced expression of exogenous genes or partial exogene silencing exhibit insufficient activation of endogenous pluripotent genes and telomerase genes, accompanied by telomere shortening with increasing passages. Moreover, telomere doublets, telomere sister chromatid exchanges and t-circles that presumably are involved in telomere lengthening by recombination also are found in porcine iPS cells. These data suggest that both telomerase-dependent and telomerase-independent mechanisms are involved in telomere reprogramming during induction and passages of porcine iPS cells, but these are insufficient, resulting in increased telomere damage and shortening, and chromosomal instability. Active exogenes might compensate for insufficient activation of endogenous genes and incomplete telomere reprogramming and maintenance of porcine iPS cells. Further understanding of telomere reprogramming and maintenance may help improve the quality of porcine iPS cells.

  13. Measurement of therapeutic photon beams-induced Cerenkov radiation generated in PMMA- and PS-based plastic optical fibers

    Science.gov (United States)

    Lee, Bongsoo; Shin, Sang Hun; Yoo, Wook Jae; Jang, Kyoung Won

    2016-10-01

    In this study, we characterized Cerenkov radiation generated in polystyrene (PS)- and polymethyl methacrylate (PMMA)-based plastic optical fibers (POFs) to select an adequate optical fiber for producing Cerenkov radiation. To determine the relationship between the absorbed dose and the intensity of Cerenkov radiation, we calculated the energy depositions of photon beams and fluxes of electrons inducing Cerenkov radiation using the Monte Carlo N-Particle eXtended code. Also, intensities of Cerenkov radiation generated in PS- and PMMA-based POFs were measured as functions of dose rate and monitor unit. At last, therapeutic photon beams-induced Cerenkov radiation in PS- and PMMA-based POFs was measured according to depths of solid water phantom.

  14. Microscale Insight into Microbial Seed Banks.

    Science.gov (United States)

    Locey, Kenneth J; Fisk, Melany C; Lennon, J T

    2016-01-01

    Microbial dormancy leads to the emergence of seed banks in environmental, engineered, and host-associated ecosystems. These seed banks act as reservoirs of diversity that allow microbes to persist under adverse conditions, including extreme limitation of resources. While microbial seed banks may be influenced by macroscale factors, such as the supply of resources, the importance of microscale encounters between organisms and resource particles is often overlooked. We hypothesized that dimensions of spatial, trophic, and resource complexity determine rates of encounter, which in turn, drive the abundance, productivity, and size of seed banks. We tested this using >10,000 stochastic individual based models (IBMs) that simulated energetic, physiological, and ecological processes across combinations of resource, spatial, and trophic complexity. These IBMs allowed realistic dynamics and the emergence of seed banks from ecological selection on random variation in species traits. Macroscale factors like the supply and concentration of resources had little effect on resource encounter rates. In contrast, encounter rates were strongly influenced by interactions between dispersal mode and spatial structure, and also by the recalcitrance of resources. In turn, encounter rates drove abundance, productivity, and seed bank dynamics. Time series revealed that energetically costly traits can lead to large seed banks and that recalcitrant resources can lead to greater stability through the formation of seed banks and the slow consumption of resources. Our findings suggest that microbial seed banks emerge from microscale dimensions of ecological complexity and their influence on resource limitation and energetic costs.

  15. Pollination-, development-, and auxin-specific regulation of gibberellin 3beta-hydroxylase gene expression in pea fruit and seeds.

    Science.gov (United States)

    Ozga, Jocelyn A; Yu, Jody; Reinecke, Dennis M

    2003-03-01

    To understand further how pollination, seeds, auxin (4-chloroindole-3-acetic acid [4-Cl-IAA]), and gibberellins (GAs) regulate GA biosynthesis in pea (Pisum sativum) fruit, we studied expression of the gene PsGA3ox1 that codes for the enzyme that converts GA(20) to biologically active GA(1) using real-time reverse transcription-polymerase chain reaction analysis. PsGA3ox1 mRNA levels were minimally detectable in prepollinated pericarps and ovules (-2 d after anthesis [DAA]), increased dramatically after pollination (0 DAA), then decreased by 1 DAA. Seed PsGA3ox1 mRNA levels increased at 4 DAA and again 8 to 12 DAA, when seed development was rapid. Pericarp PsGA3ox1 mRNA levels peaked coincidentally with rapid pod diameter expansion (6-10 DAA) to accommodate the growing seeds. The effects of seeds and hormones on the expression of pericarp PsGA3ox1 were investigated over a 24-h treatment period. Pericarp PsGA3ox1 mRNA levels gradually increased from 2 to 3 DAA when seeds were present; however, when the seeds were removed, the pericarp transcript levels dramatically declined. When 2-DAA deseeded pericarps were treated with 4-Cl-IAA, PsGA3ox1 mRNA levels peaked 4 h after hormone treatment (270-fold increase), then decreased. PsGA3ox1 mRNA levels in deseeded pericarps treated with indole-3-acetic acid or GA(3) were the same or lower than deseeded controls. These data show that PsGA3ox1 is expressed and developmentally regulated in pea pericarps and seeds. These data also show that pericarp PsGA3ox1 expression is hormonally regulated and suggest that the conversion of GA(20) to GA(1) occurs in the pericarp and is regulated by the presence of seeds and 4-Cl-IAA for fruit growth.

  16. Shallow PS-logging by high frequency wave; Koshuha wo mochiita senbu PS kenso

    Energy Technology Data Exchange (ETDEWEB)

    Nakajima, A.; Miyazawa, M.; Azuma, H. [OYO Corp., Tokyo (Japan)

    1996-05-01

    This paper describes the following matters on down-hole PS logging in shallow subsurface. Determining an elastic wave velocity structure in shallow subsurface with high accuracy by using down-hole PS logging requires reduction of errors in reading travel time. Therefore, a high-frequency vibration source was fabricated with an objective to raise frequencies of waves used for the measurement. Measurements were made on two holes, A and B, at a measurement interval of 0.5 m, whereas at the hole A a measurement was performed simultaneously by using a normal type (low-frequency) vibration source. A spectral analysis on the waveform record revealed that the frequencies with each vibration source were 127 Hz and 27 Hz for the hole A, 115 Hz for the hole B, and the S/N ratio was all the same for both holes. When the high-frequency vibration source was used, the velocity was determined at accuracy of 5% over the whole length of the shallow section. When the low-frequency vibration source was used, sections with the velocity determining error greater than 5% were found, and it was not possible to derive the velocity structure in the shallow subsurface in fine segments. 3 refs., 8 figs., 2 tabs.

  17. Seed manual: Rural household seed security

    OpenAIRE

    Neuendorf, O. (ed.)

    2004-01-01

    This training manual on Small Scale Seed Production is meant to be used by the individual farmer or farmer group, the intermediary or extension worker, as well as the trainer(s) to train, elaborate, and execute a small scale seed production project. Nine instructional chapters focus on the following topics: introduction to the basic theoretical principles of food production, planning and implementation of seed production, project preparatory phase: training of trainers; small scale seed produ...

  18. Expression of neuropeptide receptor mRNA during osteoblastic differentiation of mouse iPS cells.

    Science.gov (United States)

    Nagao, Satomi; Goto, Tetsuya; Kataoka, Shinji; Toyono, Takashi; Joujima, Takaaki; Egusa, Hiroshi; Yatani, Hirofumi; Kobayashi, Shigeru; Maki, Kenshi

    2014-12-01

    Various studies have shown a relationship between nerves and bones. Recent evidence suggests that both sensory and sympathetic nerves affect bone metabolism; however, little is known about how neuropeptides are involved in the differentiation of pluripotent stem cells into osteoblastic (OB) cells. To evaluate the putative effects of neuropeptides during the differentiation of mouse induced pluripotent stem (iPS) cells into calcified tissue-forming OB cells, we investigated the expression patterns of neuropeptide receptors at each differentiation stage. Mouse iPS cells were seeded onto feeder cells and then transferred to low-attachment culture dishes to form embryoid bodies (EBs). EBs were cultured for 4 weeks in osteoblastic differentiation medium. The expression of α1-adrenergic receptor (AR), α2-AR, β2-AR, neuropeptide Y1 receptor (NPY1-R), neuropeptide Y2 receptor (NPY2-R), calcitonin gene-related protein receptor (CGRP-R), and neurokinin 1-R (NK1-R) was assessed by reverse transcription-polymerase chain reaction (RT-PCR) and real-time PCR. Among these neuropeptide receptors, CGRP-R and β2-AR were expressed at all stages of cell differentiation, including the iPS cell stage, with peak expression occurring at the early osteoblastic differentiation stage. Another sensory nervous system receptor, NK1-R, was expressed mainly in the late osteoblastic differentiation stage. Furthermore, CGRP-R mRNA showed an additional small peak corresponding to EBs cultured for 3 days, suggesting that EBs may be affected by serum CGRP. These data suggest that the sensory nervous system receptor CGRP-R and the sympathetic nervous system receptor β2-AR may be involved in the differentiation of iPS cells into the osteoblastic lineage. It follows from these findings that CGRP and β2-AR may regulate cell differentiation in the iPS and EB stages, and that each neuropeptide has an optimal period of influence during the differentiation process. Copyright © 2014 Elsevier Ltd. All

  19. Overview of the Moral Status of iPS Cells.

    Science.gov (United States)

    Martinho, Andreia Martins

    2016-07-01

    The production of induced pluripotent stem (iPS) cells in 2006 by Takahashi and Yamanaka was a major breakthrough in stem cell research. IPS cells technology holds great promise for cell therapy, disease modelling, and drug testing, but it poses ethical questions concerning the moral status of somatic cells, which can re-gain pluripotency (iPS cells). This article provides an overview of the arguments that substantiate the debate on the moral assessment of iPS cells: potentiality argument; relational properties/standard view; and genetic basis for moral status.

  20. Structure of non-equilibrium seeded plasma excited with microwave; Micro ha reiki hiheiko seed plasma no kozo

    Energy Technology Data Exchange (ETDEWEB)

    Miyakawa, M.; Murakami, T.; Suekane, T.; Okuno, Y.; Kabashima, S. [Tokyo Institute of Technology, Tokyo (Japan)

    1996-10-20

    Structure of non-equilibrium cesium seeded argon plasma excited with microwave power is simulated numerically. The plasmas produced at suitable microwave powers are found to consist of three regimes, that is, the region limited by charged particle loss toward the wall, the full seed ionization and the diffusion limited regions. The fully ionized seed plasma is produced within the skin-depth determined by the electrical conductivity of the plasma, and the thickness of the fully ionized seed plasma depends on the seed fractions gas pressure and microwave power. 15 refs., 6 figs.

  1. Flow curve analysis of a Pickering emulsion-polymerized PEDOT:PSS/PS-based electrorheological fluid

    Science.gov (United States)

    Kim, So Hee; Choi, Hyoung Jin; Leong, Yee-Kwong

    2017-11-01

    The steady shear electrorheological (ER) response of poly(3, 4-ethylenedioxythiophene): poly(styrene sulfonate)/polystyrene (PEDOT:PSS/PS) composite particles, which were initially fabricated from Pickering emulsion polymerization, was tested with a 10 vol% ER fluid dispersed in a silicone oil. The model independent shear rate and yield stress obtained from the raw torque-rotational speed data using a Couette type rotational rheometer under an applied electric field strength were then analyzed by Tikhonov regularization, which is the most suitable technique for solving an ill-posed inverse problem. The shear stress-shear rate data also fitted well with the data extracted from the Bingham fluid model.

  2. Beam Break-up Instability in the CERN PS near Transition

    CERN Document Server

    Cappi, R; Métral, G

    2000-01-01

    Fast beam losses, due to a vertical coherent instability of high frequency, have been observed in the PS near transition energy, with the high-intensity single-bunch beam for the neutron Time-of-Flight facility (n-ToF). By increasing the longitudinal emittance, the beam could be stabilised. These phenomena can be described by the beam breakup theory, since near transition the longitudinal positions of particles are almost frozen, as in the linac case. Comparison between observations and theory, using Brandt and Gareyte's formula for single-bunch beam breakup in circular accelerators, shows good agreement.

  3. A 30 ps Timing Resolution for Single Photons with Multi-pixel Burle MCP-PMT

    Energy Technology Data Exchange (ETDEWEB)

    Va' vra, J.; Benitez, J.; Coleman, J.; Leith, D.W.G.S.; Mazaheri, G.; Ratcliff, B.; Schwiening, J.; /SLAC

    2006-07-05

    We have achieved {approx}30 psec single-photoelectron and {approx}12ps for multi-photoelectron timing resolution with a new 64 pixel Burle MCP-PMT with 10 micron microchannel holes. We have also demonstrated that this detector works in a magnetic field of 15kG, and achieved a single-photoelectron timing resolution of better than 60 psec. The study is relevant for a new focusing DIRC RICH detector for particle identification at future Colliders such as the super B-factory or ILC, and for future TOF techniques. This study shows that a highly pixilated MCP-PMT can deliver excellent timing resolution.

  4. Laser system generating 250-mJ bunches of 5-GHz repetition rate, 12-ps pulses.

    Science.gov (United States)

    Agnesi, Antonio; Braggio, Caterina; Carrà, Luca; Pirzio, Federico; Lodo, Stefano; Messineo, Giuseppe; Scarpa, Daniele; Tomaselli, Alessandra; Reali, Giancarlo; Vacchi, Carla

    2008-09-29

    We report on a high-energy solid-state laser based on a master-oscillator power-amplifier system seeded by a 5-GHz repetition-rate mode-locked oscillator, aimed at the excitation of the dynamic Casimir effect by optically modulating a microwave resonator. Solid-state amplifiers provide up to 250 mJ at 1064 nm in a 500-ns (macro-)pulse envelope containing 12-ps (micro-)pulses, with a macro/micropulse format and energy resembling that of near-infrared free-electron lasers. Efficient second-harmonic conversion allowed synchronous pumping of an optical parametric oscillator, obtaining up to 40 mJ in the range 750-850 nm.

  5. PS buildings : reinforced concrete structure for shielding "bridge" pillar

    CERN Multimedia

    CERN PhotoLab

    1956-01-01

    The PS ring traverses the region between the experimental halls South and North (buildings Nos 150 and 151) under massive bridge-shaped concrete beams. This pillar stands at the S-W end of the structure.

  6. New safety training for access to the PS complex areas

    CERN Multimedia

    2012-01-01

    Since 10/08/2012, a new course dedicated to the specific radiological risks in the accelerators of the PS complex has been available on SIR (https://sir.cern.ch/). This course complements the general classroom-based Radiation Safety training. Successful completion of the course will be obligatory and verified by the access system as from 01/11/2012 for access to the following accelerator areas: LINAC2, BOOSTER, PS and TT2. Information and reminder e-mails will be sent to all persons currently authorized to access the accelerators of the PS complex. For questions please contact the HSE unit and in particular, the Radiation Protection Group (+41227672504 or safety-rp-ps-complex@cern.ch).

  7. Organic Leek Seed Production - Securing Seed Quality

    DEFF Research Database (Denmark)

    Deleuran, L C; Boelt, B

    2011-01-01

    To maintain integrity in organic farming, availability of organically produced GM-free seed of varieties adapted to organic production systems is of vital impor-tance. Despite recent achievements, organic seed supply for a number of vegetable species is insufficient. Still, in many countries...... organic vegetable growers can get derogations to use non-organic seeds in their productions. Potentially, this could lead to the organic consumers’ loss of faith and interest in organic products. The pre-requisite for an organic vegetable production is the presence of organically produced high quality...... seeds. Tunnel production is a means of securing seed of high genetic purity and quality, and organic leek (Allium porrum L.) seed production was tested in tunnels in Denmark. The present trial focused on steckling size and in all years large stecklings had a positive effect on both seed yield...

  8. Organic leek seed production - securing seed quality

    DEFF Research Database (Denmark)

    Deleuran, Lise Christina; Boelt, Birte

    2011-01-01

    To maintain integrity in organic farming, availability of organically produced GM-free seed of varieties adapted to organic production systems is of vital impor-tance. Despite recent achievements, organic seed supply for a number of vegetable species is insufficient. Still, in many countries...... organic vegetable growers can get derogations to use non-organic seeds in their productions. Potentially, this could lead to the organic consumers’ loss of faith and interest in organic products. The pre-requisite for an organic vegetable production is the presence of organically produced high quality...... seeds. Tunnel production is a means of securing seed of high genetic purity and quality, and organic leek (Allium porrum L.) seed production was tested in tunnels in Denmark. The present trial focused on steckling size and in all years large stecklings had a positive effect on both seed yield...

  9. Study of Value Co-Creation in CoPS

    OpenAIRE

    Mingli Zhang; Jianhua Ye

    2013-01-01

    Value co-creation is associated with specific investment in the context of CoPS. The feature of CoPS decides that the study of co-creation cannot execute without regarding asset specificity. This study considers that value co-creation will be associated with specific value, which is outcome of relationship value and asset specificity. Supplier and customer have a close relation, which conducts to specific investment and then it turns to obstacle for competitors. Trust, commitment and satisfac...

  10. Motor-generator set of the PS main supply

    CERN Multimedia

    Photographic Service; CERN PhotoLab

    1968-01-01

    Already in 1964, the PS improvement programme included a new main magnet supply with more power for the longer cycles needed for slow extraction at the full energy of 26 GeV. This motor-generator set was installed in 1967 and took up service at the beginning of 1968. Regularly serviced and fitted with modern electronic regulation, it pulses the PS to this day.

  11. The Libera as a PS orbit measurement system building block

    CERN Document Server

    Belleman, J M; CERN. Geneva. AB Department

    2005-01-01

    During the year 2004, extensive tests using a Libera data processor have been made in order to study its suitability as a building block for a complete PS trajectory and orbit measurement system. The Libera consists of four fast 12-bits ADCs, a Virtex II Pro FPGA and a large memory. This note presents some of the results of the analysis of acquisitions made on a position pick-up in the CERN PS.

  12. DiPS: A Unifying Approach for developing System Software

    OpenAIRE

    Michiels, Sam; Matthijs, Frank; Walravens, Dirk; Verbaeten, Pierre

    2002-01-01

    In this paper we unify three essential features for flexible system software: a component oriented approach, self-adaptation and separation of concerns.We propose DiPS (Distrinet Protocol Stack), a component framework, which offers components, an anonymous interaction model and connectors to handle non-functional aspects such as concurrency. DiPS has effectively been used in industrial protocol stacks and device drivers.

  13. High Energy Particle Accelerators

    CERN Multimedia

    Audio Productions, Inc, New York

    1960-01-01

    Film about the different particle accelerators in the US. Nuclear research in the US has developed into a broad and well-balanced program.Tour of accelerator installations, accelerator development work now in progress and a number of typical experiments with high energy particles. Brookhaven, Cosmotron. Univ. Calif. Berkeley, Bevatron. Anti-proton experiment. Negative k meson experiment. Bubble chambers. A section on an electron accelerator. Projection of new accelerators. Princeton/Penn. build proton synchrotron. Argonne National Lab. Brookhaven, PS construction. Cambridge Electron Accelerator; Harvard/MIT. SLAC studying a linear accelerator. Other research at Madison, Wisconsin, Fixed Field Alternate Gradient Focusing. (FFAG) Oakridge, Tenn., cyclotron. Two-beam machine. Comments : Interesting overview of high energy particle accelerators installations in the US in these early years. .

  14. The PS will soon be back in operation

    CERN Multimedia

    2006-01-01

    The PS's power supply system is undergoing repairs for the accelerator to restart on 26 June. The AB Department's Power Converter Group is working flat out with Siemens to return the PS's power supply system to working order. A problem appeared on the insulation of the power cables of the rotor of the rotating machine (photo) which supplies power to the PS magnets. To prevent more significant damage to the rotating machine, the AB Department, with the approval of the CERN Management, decided to shut down the PS which had started running on 15 May. Everything is being done to restart the accelerator on 26 June. The PS's rotating machine comprises a motor coupled to a generator. The generator's rotor acts like a flywheel, supplying high-power pulses of 40 to 50 megawatts to the PS magnets. The 6 megawatt motor drives the installation at 1000 revolutions per minute and compensates only for variations in speed. It is an essential interface since it would be hard to imagine connecting such an electrical charge, p...

  15. Electroless nickel plating on polymer particles.

    Science.gov (United States)

    Fujii, Syuji; Hamasaki, Hiroyuki; Takeoka, Hiroaki; Tsuruoka, Takaaki; Akamatsu, Kensuke; Nakamura, Yoshinobu

    2014-09-15

    Near-monodisperse, micrometer-sized polypyrrole-palladium (PPy-Pd) nanocomposite-coated polystyrene (PS) particles have been coated with Ni overlayers by electroless plating in aqueous media. Good control of the Ni loading was achieved for 1.0 μm diameter PPy-Pd nanocomposite-coated PS particles and particles of up to 20 μm in diameter could also be efficiently coated with the Ni. Laser diffraction particle size analysis studies of dilute aqueous suspensions indicated that an additional water-soluble colloidal stabilizer, poly(N-vinyl pyrrolidone), in the electroless plating reaction media was crucial to obtain colloidally stable Ni-coated composite particles. Elemental microanalysis indicated that the Ni loading could be controlled between 61 and 78 wt% for the 1.0 μm-sized particles. Scanning/transmission electron microscopy studies revealed that the particle surface had a flaked morphology after Ni coating. Spherical capsules were obtained after extraction of the PS component from the Ni-coated composite particles, which indicated that the shell became rigid after Ni coating. X-ray diffraction confirmed the production of elemental Ni and X-ray photoelectron spectroscopy studies indicated the existence of elemental Ni on the surface of the composite particles. Copyright © 2014 Elsevier Inc. All rights reserved.

  16. iPS-Cinderella Story in Cell Biology

    Directory of Open Access Journals (Sweden)

    Editorial

    2010-01-01

    Full Text Available As we step through the frontiers of modern Science, we are all witnesses to the Cinderella story repeating itself in the form of the iPS. The process of re-programming adult somatic cells to derive Induced Pluripotent stem cells (iPS with the wand of transcription factors and then differentiating them back to adult somatic cells resembles the transformation of Cinderella from a Cinder girl to princess and back to a Cinder girl after the ball; but the iPS-Cinderella is the most fascinating thing ever in cell biology!From the day iPS first made its headlines when it was first produced by Shinya Yamanaka at Kyoto University in Japan, Stem Cell scientists all over the world are re- doing their experiments so far done using other sources like embryonic and adult Stem cells with the iPS cells exploring their potential to the fullest. A Stem Cell science news page without this magic word of iPS is difficult to imagine these days and Scientists have been successful in growing most of the adult Cell types from iPS cells.iPS cells was the key to solve the problems of Immune rejection and Immunosupression required when using other allogeneic Stem cell types which had baffled scientists previously. But the issues raised by scientists about the use of viruses and Oncogenes in producing iPS cells were made groundless when scientists in February 2008 published the discovery of a technique that could remove oncogenes after the induction of pluripotency and now it is possible to induce pluripotency using plasmid transfection, piggyback transposon system and piggyback transposon system combined with a non viral vector system. The word of the day is pIPS which are protein-induced Pluripotent stem cells which are iPS cells that were generated without any genetic alteration of the adult cell. This research by the group of Sheng Ding in La Jolla, California made public in April 2009 showed that the generation of poly-arginine anchors was sufficient to induce

  17. Simulation of a wide area survey for NEOs with Pan-STARRS PS1 & PS2 Telescopes

    Science.gov (United States)

    Chambers, Kenneth C.; Lilly (Schunova), Eva; Dukes, Martin Todd; Wainscoat, Richard J.

    2017-10-01

    We have performed a new survey simulation for a wide area survey with PS1 & PS2 as part of our quest to optimize the discovery rate of Near Earth Objects with the full Pan-STARRS system. The survey is intended to be as unbiased and as complete as possible given the available sky visibility and the anticipated performance of the PS1 and PS2 telescopes working together. The simulation includes a complete model of both telescopes, camera and slew overhead, sky visibility, moon phase, galactic plane exclusion, and weather. The performance of the resulting survey strategy is then evaluated using the method of Lilly et. al. 2017. This uses the Greenstreet et al. 2012 model with 50 million NEOs with absolute magnitudes 13 < H < 29 and the Moving Object Processing System (MOPS, Denneau et al. 2013) for linkages. The results are compared with other possible strategies.

  18. Organic forage seed production

    OpenAIRE

    BOELT, B.; Deleuran, L.C.

    2000-01-01

    From January 2004 only organically produced seed can be used in organic farming systems within the EU. Danish seed producers and seed companies are establishing an organic forage seed production in order to supply organic growers within the EU with high quality seed of recognised varieties. This brief paper outlines a project investigating improved cultivation and management techniques to ensure such a supply is met.

  19. Profiling the microRNA Expression in Human iPS and iPS-derived Retinal Pigment Epithelium.

    Science.gov (United States)

    Wang, Heuy-Ching; Greene, Whitney A; Kaini, Ramesh R; Shen-Gunther, Jane; Chen, Hung-I H; Cai, Hong; Wang, Yufeng

    2014-01-01

    The purpose of this study is to characterize the microRNA (miRNA) expression profiles of induced pluripotent stem (iPS) cells and retinal pigment epithelium (RPE) derived from induced pluripotent stem cells (iPS-RPE). MiRNAs have been demonstrated to play critical roles in both maintaining pluripotency and facilitating differentiation. Gene expression networks accountable for maintenance and induction of pluripotency are linked and share components with those networks implicated in oncogenesis. Therefore, we hypothesize that miRNA expression profiling will distinguish iPS cells from their iPS-RPE progeny. To identify and analyze differentially expressed miRNAs, RPE was derived from iPS using a spontaneous differentiation method. MiRNA microarray analysis identified 155 probes that were statistically differentially expressed between iPS and iPS-RPE cells. Up-regulated miRNAs including miR-181c and miR-129-5p may play a role in promoting differentiation, while down-regulated miRNAs such as miR-367, miR-18b, and miR-20b are implicated in cell proliferation. Subsequent miRNA-target and network analysis revealed that these miRNAs are involved in cellular development, cell cycle progression, cell death, and survival. A systematic interrogation of temporal and spatial expression of iPS-RPE miRNAs and their associated target mRNAs will provide new insights into the molecular mechanisms of carcinogenesis, eye differentiation and development.

  20. Particle Pollution

    Science.gov (United States)

    ... Your Health Particle Pollution Public Health Issues Particle Pollution Recommend on Facebook Tweet Share Compartir Particle pollution — ... see them in the air. Where does particle pollution come from? Particle pollution can come from two ...

  1. Pesticide-laden dust emission and drift from treated seeds during seed drilling: a review.

    Science.gov (United States)

    Nuyttens, David; Devarrewaere, Wouter; Verboven, Pieter; Foqué, Dieter

    2013-05-01

    Dressing seeds with pesticides to control pests is a widespread practice with important advantages. Recent incidents of bee losses, however, have directed attention to the emission of abraded pesticide-coated seed particles to the environment during sowing. This phenomenon of drift of pesticide dust can lead to pesticide contamination of air, water and other natural resources in crop-growing areas. This review article presents the state of the art of the phenomenon of dust emission and drift from pesticide seed dressing during sowing and its consequences. Firstly, pesticide seed treatment is defined and its pros and cons are set out, with the focus on dust, dust emission and dust drift from pesticide-coated seed. The factors affecting emission of pesticide dust (e.g. seed treatment quality, seed drilling technology and environmental conditions) are considered, along with its possible effects. The measuring techniques and protocols and models currently in use for calculating the behaviour of dust are reviewed, together with their features and limitations. Finally, possible mitigation measures are discussed, such as improving the seed quality and the use of modified seed drilling technology, and an overview of regulations and stewardship activities is given. © 2013 Society of Chemical Industry.

  2. Adsorption characteristics of mango ( magnifera indica ) seed shell ...

    African Journals Online (AJOL)

    Adsorption characteristics of mango (Magnifera indica) seed shell activated carbon for phenol rem-oval from wastewater were examined by experimental single stage batch operation. Contact time, stirring rate, adsorbent dose, pH, initial phenol concentration, carbon particle size and impregnation ratio of ZnCl2 to the seed ...

  3. Efficient energy absorption of intense ps-laser pulse into nanowire target

    Energy Technology Data Exchange (ETDEWEB)

    Habara, H.; Honda, S.; Katayama, M.; Tanaka, K. A. [Graduate School of Engineering, Osaka University, 2-1 Suita, Osaka 565-0871 (Japan); Sakagami, H. [National Institute for Fusion Science, Toki, Gifu 509-5292 (Japan); Nagai, K. [Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology, Nagatsuda 4259, Midori-ku, Yokohama 226-8503, Kanagawa (Japan)

    2016-06-15

    The interaction between ultra-intense laser light and vertically aligned carbon nanotubes is investigated to demonstrate efficient laser-energy absorption in the ps laser-pulse regime. Results indicate a clear enhancement of the energy conversion from laser to energetic electrons and a simultaneously small plasma expansion on the surface of the target. A two-dimensional plasma particle calculation exhibits a high absorption through laser propagation deep into the nanotube array, even for a dense array whose structure is much smaller than the laser wavelength. The propagation leads to the radial expansion of plasma perpendicular to the nanotubes rather than to the front side. These features may contribute to fast ignition in inertial confinement fusion and laser particle acceleration, both of which require high current and small surface plasma simultaneously.

  4. The TDCpix readout ASIC: A 75ps resolution timing front-end for the NA62 Gigatracker hybrid pixel detector

    CERN Document Server

    Kluge, A; Bonacini, S; Jarron, P; Kaplon, J; Morel, M; Noy, M; Perktold, L; Poltorak, K

    2013-01-01

    The TDCpix is a novel pixel readout ASIC for the NA62 Gigatracker detector. NA62 is a new experiment being installed at the CERN Super Proton Synchrotron. Its Gigatracker detector shall provide on-beam tracking and time stamping of individual particles with a time resolution of 150 ps rms. It will consist of three tracking stations, each with one hybrid pixel sensor. The peak fl ow of particles crossing the detector modules reaches 1.27 MHz/mm 2 for a total rate of about 0.75 GHz. Ten TDCpix chips will be bump-bonded to every silicon pixel sensor. Each chip shall perform time stamping of 100 M particle hits per second with a detection ef fi ciency above 99% and a timing accuracy better than 200 ps rms for an overall three-station-setup time resolution of better than 150 ps. The TDCpix chip has been designed in a 130 nm CMOS technology. It will feature 45 40 square pixels of 300 300 μ m 2 and a complex End of Column peripheral region including an array of TDCs based on DLLs, four high speed serializers, a low...

  5. Basic performance of a position-sensitive tissue-equivalent proportional chamber (PS-TEPC)

    Energy Technology Data Exchange (ETDEWEB)

    Kishimoto, Yuji, E-mail: yuji.kishimoto@kek.jp [High Energy Accelerator Research Organization (KEK) (Japan); Sasaki, Shinichi; Saito, Kiwamu; Takahashi, Kazutoshi [High Energy Accelerator Research Organization (KEK) (Japan); Doke, Tadayoshi [Waseda University (Japan); Miuchi, Kentaro [Kobe University (Japan); Fuse, Tetsuhito; Nagamatsu, Aiko [Japan Aerospace Exploration Agency (JAXA) (Japan); Uchihori, Yukio; Kitamura, Hisashi [National Institute of Radiological Sciences (Japan); Terasawa, Kazuhiro [Keio University (Japan); Japan Aerospace Exploration Agency (JAXA) (Japan)

    2013-12-21

    The characteristics and performance of the prototype of a Position-Sensitive Tissue-Equivalent Proportional Chamber (PS-TEPC), consisting of a miniaturized micro-pixel chamber (μ-PIC), were investigated. The gas–gain curve of the μ-PIC and an approximate function with which to convert measured collected charge into the primary charge ionized by incident heavy-ion particles were obtained. The measured Linear Energy Transfer (LET) indicated good agreement with that of the injected beam calculated using SRIM2008 code in the LET range from 0.4 to 180keV/μm, which roughly covers the whole LET range required for space dosimeters. As expected, the measured energy resolution improved with increasing LET of the injected beam. It was estimated to be 27% for 0.4keV/μm, which is just about the LET of minimum-ionizing particles. Consequently, it was confirmed that the PS-TEPC exhibits no significant undesirable feature but is well suited for space dosimetry.

  6. Characterization of crosslinked polystyrene(PS) beads in SBR matrix

    Energy Technology Data Exchange (ETDEWEB)

    Cha, Yoon-Jong; Choe, Soonja [Inha Univ. (Korea, Republic of)

    1995-12-01

    Monodisperse sized crosslinked polystyrene(PS) beads were prepared by a reaction of semibatch emulsion polymerization with styrene monomer, divinylbenzene(DVB) crosslinking agent and potassium persulfate(K{sub 2}S{sub 2}O{sub 9}) initiator in the absence of emulsifier. The glass transition temperature(T{sub g}) and the mean diameter of the beads were increased from 100{degrees}C to 135{degrees}C and from 402 nm to 532 nm, respectively, for an incorporation of 2 to 10 mol% DVB. Crosslinking density was also linearly increased with DVB content. SEM microphotographs of SBR composite filled with various contents of PS beads revealed that PS beads are relatively well dispersed without changing the spherical shape of the beads in all range of compositions. In stress-strain analysis, elongation at break and tensile strength of SBR composite were increased with the bead content. Applicability of the PS beads as a filler in SBR matrix is tested by plotting Mooney-Rivlin or Guth-Smallwood equations. However, mechanical properties of the composite with the beads were not so excellent as those of the composite with carbon black. Crosslinked PS beads are still tentative as a white color reinforcing filler on SBR matrix.

  7. The PS complex produces the nominal LHC beam

    CERN Document Server

    Benedikt, Michael; Borburgh, J; Cappi, R; Chanel, M; Chohan, V; Cyvoct, G; Garoby, R; Grier, D G; Gruber, J; Hancock, S; Hill, C E; Jensen, E; Krusche, A; Lindroos, M; Métral, Elias; Métral, G; Metzmacher, K D; Olsfors, J; Pedersen, F; Raich, U; Riunaud, J P; Royer, J P; Sassowsky, M; Schindl, Karlheinz; Schönauer, Horst Otto; Thivent, M; Ullrich, H M; Völker, F V; Vretenar, Maurizio; Barnes, M; Blackmore, E W; Cifarelli, F; Clark, G; Jones, F; Koscielniak, Shane Rupert; Mammarella, F; Mitra, A; Poirier, R; Reiniger, K W; Ries, T C

    2000-01-01

    The LHC [1] will be supplied, via the SPS, with protons from the pre-injector chain comprising Linac2, PS Booster (PSB) and PS. These accelerators have under-gone a major upgrading programme [2] during the last five years so as to meet the stringent requirements of the LHC. These imply that many high-intensity bunches of small emittance and tight spacing (25 ns) be available at the PS extraction energy (25 GeV). The upgrading project involved an increase of Linac2 current, new RF systems in the PSB and the PS, raising the PSB energy from 1 to 1.4 GeV, two-batch filling of the PS and the installation of high-resolution beam profile measurement devices. With the project entering its final phase and most of the newly installed hardware now being operational, the emphasis switches to producing the nominal LHC beam and tackling the associated beam physics problems. While a beam with transverse characteristics better than nominal has been obtained, the longitudinal density still needs to be increased. An alternativ...

  8. Pressure Monitoring Using Hybrid fs/ps Rotational CARS

    Science.gov (United States)

    Kearney, Sean P.; Danehy, Paul M.

    2015-01-01

    We investigate the feasibility of gas-phase pressure measurements at kHz-rates using fs/ps rotational CARS. Femtosecond pump and Stokes pulses impulsively prepare a rotational Raman coherence, which is then probed by a high-energy 6-ps pulse introduced at a time delay from the Raman preparation. Rotational CARS spectra were recorded in N2 contained in a room-temperature gas cell for pressures from 0.1 to 3 atm and probe delays ranging from 10-330 ps. Using published self-broadened collisional linewidth data for N2, both the spectrally integrated coherence decay rate and the spectrally resolved decay were investigated as means for detecting pressure. Shot-averaged and single-laser-shot spectra were interrogated for pressure and the accuracy and precision as a function of probe delay and cell pressure are discussed. Single-shot measurement accuracies were within 0.1 to 6.5% when compared to a transducer values, while the precision was generally between 1% and 6% of measured pressure for probe delays of 200 ps or more, and better than 2% as the delay approached 300 ps. A byproduct of the pressure measurement is an independent but simultaneous measurement of the gas temperature.

  9. Shaping metal nanocrystals through epitaxial seeded growth

    Energy Technology Data Exchange (ETDEWEB)

    Habas, Susan E.; Lee, Hyunjoo; Radmilovic, Velimir; Somorjai,Gabor A.; Yang, Peidong

    2008-02-17

    Morphological control of nanocrystals has becomeincreasingly important, as many of their physical and chemical propertiesare highly shape-dependent. Nanocrystal shape control for both single andmultiple material systems, however, remains fairly empirical andchallenging. New methods need to be explored for the rational syntheticdesign of heterostructures with controlled morphology. Overgrowth of adifferent material on well-faceted seeds, for example, allows for the useof the defined seed morphology to control nucleation and growth of thesecondary structure. Here, we have used highly faceted cubic Pt seeds todirect the epitaxial overgrowth of a secondary metal. We demonstrate thisconcept with lattice matched Pd to produce conformal shape-controlledcore-shell particles, and then extend it to lattice mismatched Au to giveanisotropic growth. Seeding with faceted nanocrystals may havesignificant potential towards the development of shape-controlledheterostructures with defined interfaces.

  10. Porous titanium particles for acetabular reconstruction in total hip replacement show extensive bony armoring after 15 weeks

    NARCIS (Netherlands)

    Walschot, L.H.; Aquarius, R.; Verdonschot, N.J.; Buma, P.; Schreurs, B.W.

    2014-01-01

    Background and purpose - The bone impaction grafting technique restores bone defects in total hip replacement. Porous titanium particles (TiPs) are deformable, like bone particles, and offer better primary stability. We addressed the following questions in this animal study: are impacted TiPs

  11. Effects of Particle Size, Applied Pressure and Pressing Time on the ...

    African Journals Online (AJOL)

    Effects of Particle Size, Applied Pressure and Pressing Time on the Yield of Oil Expressed from Almond Seed. ... The results obtained can be used in the design of a suitable process and machine for the expression of oil from almond seed. Keywords: Almond oil, almond seed, applied pressure, particle size, pressing time.

  12. Spectroscopic discernment of seed cotton trash

    Science.gov (United States)

    Detection and identification of foreign material in harvested seed cotton is required for efficient removal by ginning. Trash particles remaining within the cotton fibers can detrimentally impact the quality of resulting textile products. Luminescence has been investigated as a potential tool for su...

  13. The evolution of seeds.

    Science.gov (United States)

    Linkies, Ada; Graeber, Kai; Knight, Charles; Leubner-Metzger, Gerhard

    2010-06-01

    The evolution of the seed represents a remarkable life-history transition for photosynthetic organisms. Here, we review the recent literature and historical understanding of how and why seeds evolved. Answering the 'how' question involves a detailed understanding of the developmental morphology and anatomy of seeds, as well as the genetic programs that determine seed size. We complement this with a special emphasis on the evolution of dormancy, the characteristic of seeds that allows for long 'distance' time travel. Answering the 'why' question involves proposed hypotheses of how natural selection has operated to favor the seed life-history phenomenon. The recent flurry of research describing the comparative biology of seeds is discussed. The review will be divided into sections dealing with: (1) the development and anatomy of seeds; (2) the endosperm; (3) dormancy; (4) early seed-like structures and the transition to seeds; and (5) the evolution of seed size (mass). In many cases, a special distinction is made between angiosperm and gymnosperm seeds. Finally, we make some recommendations for future research in seed biology.

  14. Final Results on the CERN PS Electrostatic Septa Consolidation Program

    CERN Document Server

    Borburgh, Jan; Bobbio, Piero; Carlier, Etienne; Hourican, Michael; Masson, Thierry; Müller, Tania; Prost, Antoine; Crescenti, Massimo

    2003-01-01

    The CERN PS electrostatic septum consolidation program is coming to completion after almost 4 years of development. The program was started to fulfil the increased requirements on vacuum performance and the need to reduce the time necessary for maintenance interventions. The new design of septum 31, used for the so-called 'continuous transfer' 5-turn extraction, and the related construction issues will be presented together with the operational experience gained during the PS 2002 run. In addition, the experience of two years of operation with the new generation septum 23, used for a resonant slow extraction, will be briefly discussed. The continued development undertaken since its installation in the PS ring in 2001 will also be described.

  15. SAFETY: STRICTER CONTROLS IN CONTROLLED AREAS IN THE PS

    CERN Document Server

    G. Daems

    2001-01-01

    The PS accelerators will soon stop for several months. Work will take place in controlled areas in the PS and will involve many people who are not always aware of the risks associated with the work sites. To guarentee the safety of these workers, the following two measures will be applied: everyone working in a controlled zone - Linacs, PSB, and PS machines tunnels, and transfer lines - must wear, visibly, his CERN access card and his film badge. the CERN access card and the film badge will only be issued after following a basic safety course. Regular checks will be carried out during the shutdown. Anyone without these two items on their person will be obliged to leave the area immediately.

  16. Electrophysical properties of PMN-PT-PS-PFN:Li ceramics

    Directory of Open Access Journals (Sweden)

    R. Skulski

    2013-01-01

    Full Text Available We present the technology of obtaining and the electrophysical properties of a multicomponent material 0.61PMN-0.20PT-0.09PS-0.1PFN:Li (PMN-PT-PS-PFN:Li. The addition of PFN into PMN-PT decreases the temperature of final sintering which is very important during technological process (addition of Li decreases electric conductivity of PFN. Addition of PS i.e., PbSnO3 (which is unstable in ceramic form permits to shift the temperature of the maximum of dielectric permittivity. One-step method of obtaining ceramic samples from oxides and carbonates has been used. XRD, microstructure, scanning calorimetry measurements and the main dielectric, ferroelectric and electromechanical properties have been investigated for the obtained samples.

  17. Assuring the quality of quality assurance: seeding abnormal slides into the negative Papanicolaou smears that will be rapid rescreened.

    Science.gov (United States)

    Clarke, Joanne; Thurloe, Julia K; Bowditch, Ron C; Roberts, Jennifer M

    2008-10-25

    Rapid rescreening (RR) of negative Papanicolaou smears (PS) is used in many countries as a quality-assurance measure. Seeding of abnormal slides has been suggested as a way to increase the sensitivity of this procedure. Since 2004, the authors have carried out RR with seeding before issuing reports. In this article, they describe their experience. Abnormal seeds were sourced from the previous day's high-grade cases, both squamous and glandular. Slides were evaluated for the 'degree of difficulty' (which was defined as the number of fields required to find (fields-to-find [FTF]) the abnormality), relabeled, and redotted to make them indistinguishable from the routine RR work. The number of seeds found/missed, the identity of the screener, the type of seeded abnormality, the degree of difficulty of the seed, and the mapping technique used all were recorded. The cytologists also were surveyed about their views on seeding. Overall, 14.8% of 3082 high-grade seeds were missed during RR. There was no relation between seeds missed and the mapping technique used. However, the difficulty of the seed was relevant to the number missed and ranged from 8.3% when the FTF was 10 (P = .000). The difference between intraepithelial seeds and invasive seeds was significant for squamous seeds (P = .031) but not for glandular seeds. Glandular seeds also were more likely to be missed than squamous seeds (23.1% vs 14.3%; P = .002). Most cytologists believed that seeding was a good idea and that seeds increased their level of vigilance. The authors' experience demonstrated that routine seeding is practicable for both conventional and liquid-based slides. With the advent of the human papillomavirus vaccine, abnormalities will become rarer, and seeding will be necessary to maintain the alertness of cytologists. (c) 2008 American Cancer Society.

  18. Effect of interfaces on the melting of PEO confined in triblock PS-b-PEO-b-PS copolymers.

    Science.gov (United States)

    Beaudoin, E; Phan, T N T; Robinet, M; Denoyel, R; Davidson, P; Bertin, D; Bouchet, R

    2013-08-27

    Block copolymers form nanostructures that have interesting physical properties because they combine, for a single compound, the complementary features brought by each block. However, in order to fully exploit these properties, the physical state of each kind of domain must be precisely controlled. In this work, triblock PS-b-PEO-b-PS copolymers consisting of a central poly(ethylene oxide) (PEO) block covalently bonded to polystyrene (PS) blocks were synthesized by Atom Transfer Radical Polymerization. Their morphology was investigated by X-ray scattering and TEM experiments whereas their thermodynamic behavior was characterized by DSC. A strong decrease of both the melting temperature and the degree of crystallinity of PEO, due to its confinement between the PS domains, was observed and analyzed with a modified Gibbs-Thomson equation, following the approaches used for fluids confined in porous media. The existence of an amorphous bound layer, a few nanometers thick, at the PEO/PS interface, that does not undergo any phase transition in the temperature range investigated, accounts for both the melting temperature depression and the decrease of crystallinity upon confinement. This interfacial layer may significantly affect the mechanical and transport properties of these block copolymers that find applications as solid polymer electrolytes in batteries for example. Moreover, the value obtained for the solid PEO/liquid PEO surface tension is lower than those previously published but is thermodynamically consistent with the surface tensions of polymers at the solid/vapor and liquid/vapor interfaces.

  19. Inauguration of POPS: the new power system of the PS

    CERN Multimedia

    Maximilien Brice

    2010-01-01

    Pictures 03 and 04 : The team from the Electrical Power Converters Group (TE/EPC) is joined by the Director of Accelerators, the heads of the BE, TE and FI departments, CERN managers and Converteam representatives in a group portrait in front of three of the containers that house the capacitor banks of the PS's new power supply system, POPS. Pictures 01, 06 and 07 : Magid-Michel Saikaly, energy and infrastructure director at Converteam, receives a prize from Steve Myers, Director of Accelerators at CERN, for the development and fabrication of the new electrical power system for the PS, called POPS.

  20. The Septa for LEIR Extraction and PS Injection

    CERN Document Server

    Borburgh, J; Masson, T; Prost, A

    2006-01-01

    The Low Energy Ion Ring (LEIR) is part of the CERN LHC injector chain for ions. The LEIR extraction uses a pulsed magnetic septum, clamped around a metallic vacuum chamber. Apart from separating the ultra high vacuum in the LEIR ring from the less good vacuum in the transfer line to the PS this chamber also serves as magnetic screen and retains the septum conductor in place. The PS ion injection septum consists of a pulsed laminated magnet under vacuum, featuring a single-turn water cooled coil and a remote positioning system. The design, the construction and the commissioning of both septa are described.

  1. Magnetic separation of antibiotics by electrochemical magnetic seeding

    Energy Technology Data Exchange (ETDEWEB)

    Ihara, I; Toyoda, K [Department of Agricultural Engineering and Socio Economics, Kobe University, Nada, Kobe 657-8501 (Japan); Beneragama, N; Umetsu, K [Department of Animal Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555 (Japan)], E-mail: ihara@port.kobe-u.ac.jp

    2009-03-01

    Magnetic separation of several classes of antibiotics was investigated using electrochemical magnetic seeding. Electrocoagulation with a sacrificial anode followed by addition of magnetite particles was applied for the magnetic seeding of antibiotics. With electrochemical magnetic seeding using an iron anode, tetracycline antibiotics (oxytetracycline, chlortetracycline, doxycycline and tetracycline) and cephalosporin antibiotic (cefdinir) were rapidly removed from synthetic wastewater by magnetic separation using a neodymium magnet. Iron and aluminium anodes were suitable for magnetic seeding of the antibiotics. The results indicated that the ability of antibiotics to form strong complex with iron and aluminium allowed the higher removal by magnetic separation. This method would be appropriate for rapid treatment of antibiotics in wastewater.

  2. 7 CFR 201.18 - Other agricultural seeds (crop seeds).

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Other agricultural seeds (crop seeds). 201.18 Section... SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) FEDERAL SEED ACT FEDERAL SEED ACT REGULATIONS Labeling Agricultural Seeds § 201.18 Other agricultural seeds (crop...

  3. Efficiency of seed production in southern pine seed orchards

    Science.gov (United States)

    David L. Bramlett

    1977-01-01

    Seed production in southern pine seed orchards can be evaluated by estimating the efficiency of four separate stages of cone, seed, and seedling development. Calculated values are: cone efficiency (CE), the ratio of mature cones to the initial flower crop; seed efficiency (SE), the ratio of filled seeds per cone to the seed potential; extraction efficiency (EE), the...

  4. Healthy food trends -- chia seeds

    Science.gov (United States)

    ... Chia seeds; Wellness - Chia seeds References Academy of Nutrition and Dietetics. What are chia seeds? Updated February 5, 2014. www.eatright.org/resource/food/vitamins-and-supplements/nutrient-rich-foods/what-are-chia-seeds . Accessed ...

  5. Grape Seed Extract

    Science.gov (United States)

    ... seeds, which are by-products of the wine industry. Grape seed extract is available in capsules and ... take anticoagulants (blood thinners), such as warfarin or aspirin. Keep in Mind Tell all your health care ...

  6. Seed dispersal in fens

    NARCIS (Netherlands)

    Middleton, Beth; van Diggelen, Rudy; Jensen, Kai

    2006-01-01

    Question: How does seed dispersal reduce fen isolation and contribute to biodiversity? Location: European and North American fens. Methods: This paper reviews the literature on seed dispersal to fens. Results: Landscape fragmentation may reduce dispersal opportunities thereby isolating fens and

  7. Fabrication of metal half-shells using colloidal particle monolayer and their application in surface-enhanced Raman scattering.

    Science.gov (United States)

    Taniguchi, Yuichi; Endo, Hiroshi; Kawai, Takeshi

    2012-01-01

    Three types of Au shells, an isolated half-shell, one-dimensional strings of shells, and two-dimensional films, were fabricated by using a monolayer of polystyrene (PS) particles with diameters of 213, 560, and 1360 nm. The three types of Au shells that were removed from the PS particle monolayer and the as-deposited Au shells that adhered to PS particles were modified with 4-mercaptopyridine for use as platforms for surface-enhanced Raman scattering (SERS). We examined the effects of the shapes and sizes of Au shells on their SERS efficiency and found that the Au shells exhibited strong SERS signals and that Au shells prepared by using 560-nm PS particles were the most suitable platform for SERS at both 632.8- and 785-nm excitations. Further, we found that SERS enhancements depended on the shape of Au shells and on whether Au shells adhered to PS particles or not.

  8. Pollination-, Development-, and Auxin-Specific Regulation of Gibberellin 3β-Hydroxylase Gene Expression in Pea Fruit and Seeds1

    Science.gov (United States)

    Ozga, Jocelyn A.; Yu, Jody; Reinecke, Dennis M.

    2003-01-01

    To understand further how pollination, seeds, auxin (4-chloroindole-3-acetic acid [4-Cl-IAA]), and gibberellins (GAs) regulate GA biosynthesis in pea (Pisum sativum) fruit, we studied expression of the gene PsGA3ox1 that codes for the enzyme that converts GA20 to biologically active GA1 using real-time reverse transcription-polymerase chain reaction analysis. PsGA3ox1 mRNA levels were minimally detectable in prepollinated pericarps and ovules (−2 d after anthesis [DAA]), increased dramatically after pollination (0 DAA), then decreased by 1 DAA. Seed PsGA3ox1 mRNA levels increased at 4 DAA and again 8 to 12 DAA, when seed development was rapid. Pericarp PsGA3ox1 mRNA levels peaked coincidentally with rapid pod diameter expansion (6–10 DAA) to accommodate the growing seeds. The effects of seeds and hormones on the expression of pericarp PsGA3ox1 were investigated over a 24-h treatment period. Pericarp PsGA3ox1 mRNA levels gradually increased from 2 to 3 DAA when seeds were present; however, when the seeds were removed, the pericarp transcript levels dramatically declined. When 2-DAA deseeded pericarps were treated with 4-Cl-IAA, PsGA3ox1 mRNA levels peaked 4 h after hormone treatment (270-fold increase), then decreased. PsGA3ox1 mRNA levels in deseeded pericarps treated with indole-3-acetic acid or GA3 were the same or lower than deseeded controls. These data show that PsGA3ox1 is expressed and developmentally regulated in pea pericarps and seeds. These data also show that pericarp PsGA3ox1 expression is hormonally regulated and suggest that the conversion of GA20 to GA1 occurs in the pericarp and is regulated by the presence of seeds and 4-Cl-IAA for fruit growth. PMID:12644664

  9. The OMERACT psoriatic arthritis magnetic resonance imaging scoring system (PsAMRIS): definitions of key pathologies, suggested MRI sequences, and preliminary scoring system for PsA Hands

    DEFF Research Database (Denmark)

    Østergaard, Mikkel; McQueen, Fiona; Wiell, Charlotte

    2009-01-01

    This article describes a preliminary OMERACT psoriatic arthritis magnetic resonance image scoring system (PsAMRIS) for evaluation of inflammatory and destructive changes in PsA hands, which was developed by the international OMERACT MRI in inflammatory arthritis group. MRI definitions of important...... pathologies in peripheral PsA and suggestions concerning appropriate MRI sequences for use in PsA hands are also provided....

  10. (Cuminum cyminum L.) seeds

    African Journals Online (AJOL)

    lenovo

    2013-08-19

    Aug 19, 2013 ... Key words: Cumin (Cuminum cyminum L.), Apiaceae, seed, fatty acids composition, petroselinic acid, maturation. ... Harvest dates, days after flowering, fruit colour and state of maturity, relative moisture and oil contents of cumin seeds ..... composition of cactus pear seed compared with cotton and grape.

  11. Needs of Seeds

    Science.gov (United States)

    Keeley, Page

    2011-01-01

    The "Needs of Seeds" formative assessment probe can be used to find out whether students recognize that seeds have needs both similar to and different from plants and other living organisms (Keeley, Eberle, and Tugel 2007). The probe reveals whether students overgeneralize the needs of seeds by assuming they have the same needs as the adult plants…

  12. Seed Proteomics"

    Science.gov (United States)

    Proteomic analysis of seeds encounters some specific problems that do not impinge on analyses of other plant cells, tissues, or organs. There are anatomic considerations. Seeds comprise the seed coat, the storage organ(s), and the embryonic axis. Are these to be studied individually or as a compo...

  13. Seed development and carbohydrates

    NARCIS (Netherlands)

    Wittich, P.E.

    1998-01-01

    Seeds assure the plant the onset of a next generation and a way of dispersal. They consist of endosperm and an embryo (originating from gametophytic tissue), enveloped by a seed coat (sporophytic tissue). Plants generate different types of seeds. For instance, the endosperm may either be

  14. Seeds to survive

    NARCIS (Netherlands)

    Groot, S.P.C.

    2002-01-01

    Seeds are important for man, either as propagation material of crops or directly for the production of foods, fodder and drinks. The natural function of seeds is dispersal of its genes to successive generations. Survival mechanisms seed have evolved sometimes interfere with those preferred by

  15. Nanostructured silicon particles for medical applications.

    Science.gov (United States)

    Kleps, Irina; Ignat, Teodora; Miu, Mihaela; Craciunoiu, Florea; Trif, Mihaela; Simion, Monica; Bragaru, Adina; Dinescu, Adrian

    2010-04-01

    Porous silicon (PS) which has different properties from the bulk material due to the quantum confinement effects is beside other physical properties (e.g., light emitting) bioactive or even bioresorbable. The aim of this paper is to optimise the experimental conditions for the fabrication of nanostructured Si particles and to find the best methods for attaching on its surface molecules of therapeutic interest. The selective porosification has been performed using (i) a dielectric/metallic masking layer micropatterned with corresponding etching windows; (ii) a controlled diffusion process leading to n-type islands into p-type Si substrate. The PS particles were detached from the Si substrate by switching the electrochemical etching conditions from porosification towards the electropolishing regime. Also, similar results were obtained by fabrication of PS multilayer structures subjected to an additional ultrasonation process. Different organic molecules with antitumoral effect, such as chondroitin sulphate (a sulphated glycosaminoglycan), lactoferrin (globular protein with antimicrobial activity) and N-butyldeoxynojirimycin (an imino sugar that inhibits the growth of the CT-2A brain tumour) were covalently attached on the PS particle surface using 3-aminopropyltriethoxysilane (APTS) molecule as linker. Furthermore, to complete the administration/therapy of drugs, for microparticle targeting and imaging, Fe3O4 nanoparticles were integrated in PS matrix by co-precipitation from a solution of iron salts (Fe3+/Fe2+) in alkaline medium. Microscopic and spectroscopic analyses have been used to characterize the Si microparticles. Tumoral cells were cultivated on the nanostructured PS particles and a significant decrease of the cells density was observed on all investigated samples comparatively with the blank substrate without antitumoral molecules.

  16. Water Atomization of Barium Fluoride: Calcium Fluoride for Enhanced Flow Characteristics of PS304 Feedstock Powder Blend

    Science.gov (United States)

    Stanford, Malcolm K.; DellaCorte, Christopher

    2003-01-01

    PS304 is a plasma spray deposited solid lubricant coating with feedstock composed of NiCr, Cr2O3, Ag, and BaF2-CaF2 powders. The effects of rounded BaF2-CaF2 particles on the gravity-fed flow characteristics of PS304 feedstock have been investigated. The BaF2-CaF2 powder was fabricated by water atomization using four sets of process parameters. Each of these powders was then characterized by microscopy and classified by screening to obtain 45 to 106 micron particles and added incrementally from 0 to 10 wt% to the other constituents of the PS304 feedstock, namely nichrome, chromia, and silver powders. The relationship between feedstock flow rate, measured with the Hall flowmeter, and concentration of fluorides was found to be linear in each case. The slopes of the lines were between those of the linear relationships previously reported using angular and spherical fluorides and were closer to the relationship predicted using the rule of mixtures. The results offer a fluoride fabrication technique potentially more cost-effective than gas atomization processes or traditional comminution processes.

  17. Assessment of Landslide Hazard Using PS-InSAR and Distinct Element Method: A Case Study in Central Taiwan

    Science.gov (United States)

    Tung, Y. H.; Hu, J. C.

    2016-12-01

    Our studies focus on the Gin-Yin Village, located at central Taiwan, where is an area vulnerable to landslide hazard. We adopted L-band ALOS SAR data to generate the line of sight (LOS) velocities in study area using PS-InSAR technique constrained by continuous GPS data in the period from 2007 to 2010. From the preliminary results of PS-InSAR, we noticed several LOS velocity trends related to potential landslides and a LOS velocity gap in the northeastern of the village. In addition, the borehole inclinometers installed by Central Geological Survey, recorded a slip depth more than 120 m just in the northeastern of the village. Based on the results of PS-InSAR, records of inclinometers and field geological study, we adopt 2-D and 3-D numerical simulations by using Particle Flow Code to investigate scenario-based runout paths, particle velocities and landslide-affected areas, which are useful information for decision support and future landslide hazard assessment. The area of the potential sliding slope is about 14 hectares, and the dipping of the potential sliding surface might be varied from 21 to 5 degree, the critical frictional coefficient to cause sliding would be 0.45. Once the slope slides, the buildings on the terrace may be destroyed or even be buried by debris within 60 seconds.

  18. Wound induced tanscriptional regulation of benzylisoquinoline pathway and characterization of wound inducible PsWRKY transcription factor from Papaver somniferum.

    Directory of Open Access Journals (Sweden)

    Sonal Mishra

    Full Text Available Wounding is required to be made in the walls of the green seed pod of Opium poppy prior exudation of latex. To withstand this kind of trauma plants regulate expression of some metabolites through an induced transcript level. 167 unique wound-inducible ESTs were identified by a repetitive round of cDNA subtraction after 5 hours of wounding in Papaver somniferum seedlings. Further repetitive reverse northern analysis of these ESTs revealed 80 transcripts showing more than two fold induction, validated through semi-quantitative RT-PCR & real time expression analysis. One of the major classified categories among identified ESTs belonged to benzylisoquinoline transcripts. Tissue specific metabolite analysis of benzylisoquinoline alkaloids (BIAs in response to wounding revealed increased accumulation of narcotine and papaverine. Promoter analysis of seven transcripts of BIAs pathway showed the presence of W-box cis-element with the consensus sequence of TGAC, which is the proposed binding site for WRKY type transcription factors. One of the Wound inducible 'WRKY' EST isolated from our subtracted library was made full-length and named as 'PsWRKY'. Bacterially expressed PsWRKY interacted with the W-box element having consensus sequence TTGACT/C present in the promoter region of BIAs biosynthetic pathway genes. PsWRKY further activated the TYDC promoter in yeast and transiently in tobacco BY2 cells. Preferential expression of PsWRKY in straw and capsule and its interaction with consensus W-box element present in BIAs pathway gene transcripts suggest its possible involvement in the wound induced regulation of BIAs pathway.

  19. Optimization of protease production by an actinomycete Strain, PS ...

    African Journals Online (AJOL)

    Actinomycetes were isolated from the sediment samples of an estuarine shrimp pond located along the south east coast of India. During the investigation, a total of 28 strains of actinomycetes were isolated and examined for their protease activity. Among them, one strain PS-18A which was tentatively identified as ...

  20. Seismic receiver function interpretation: Ps splitting or anisotropic underplating?

    Science.gov (United States)

    Liu, Zhen; Park, Jeffrey

    2017-03-01

    Crustal anisotropy is crucial to understanding the evolutionary history of Earth's lithosphere. Shear wave splitting of Moho P-to-S converted phases in receiver functions (RFs) have been often used to study crustal anisotropy. Harmonic variation of Moho Ps phases in delay times are used to infer splitting parameters of averaged anisotropy in the crust. However, crustal anisotropy may distribute at various levels within the crust due to complex deformational processes. Layered anisotropy requires careful investigation of the distribution of anisotropy before interpreting Moho Ps splitting. In this study, we show results from stations ARU in Russia, KIP in the Hawaiian Islands and LSA in Tibetan Plateau, where layered anisotropy is constrained well by intracrust Ps conversions at high frequencies using a harmonic-decomposition technique. Anisotropic velocity models are inferred by forward-modeling decomposed RF waveforms. We suggest that the harmonic variation of Moho Ps phases should always be investigated to check for anisotropic layering using RFs with frequency content above 1 Hz, rather than simply reporting averaged anisotropy of the whole crust.

  1. Optimization of protease production by an actinomycete Strain, PS ...

    African Journals Online (AJOL)

    STORAGESEVER

    Isolation Agar Medium in duplicate Petri plates. To minimize ... on the Petri plates were counted from 5th day onwards, up to 28th .... After the dialysis, the volume was measured and analyzed for proteins and stored in deep freezer. Taxonomic investigation. The genus level identification was made for the strain PS-18A using ...

  2. Framing Retention for Institutional Improvement: A 4 Ps Framework

    Science.gov (United States)

    Kalsbeek, David H.

    2013-01-01

    A 4 Ps framework for student retention strategy is a construct for reframing the retention discussion in a way that enables institutional improvement by challenging some conventional wisdom and prevailing perspectives that have characterized retention strategy for years. It opens new possibilities for action and improvement by suggesting that…

  3. Multipole stack for the 4 rings of the PS Booster

    CERN Multimedia

    CERN PhotoLab

    1976-01-01

    The PS Booster (originally 800 MeV, now 1.4 GeV) saw first beam in 1972, routine operation began in 1973. The strive for ever higher intensities required the addition of multipoles. Manufacture of 8 stacks of multipoles was launched in 1974, for installation in 1976. For details, see 7511120X.

  4. The Swelling Behaviour of Polystyrene (PS)/ Polyvinylacetate (Pvac ...

    African Journals Online (AJOL)

    The effect of the variation of the type of solvent responsible for the differences in the swelling kinetics of Polystyrene (PS) and Polyvinyl acetate (PVAc) blends was studied. The results showed that the nature of solvent control or affects the degree of swelling. Also, 1-V characteristics at temperature range of 323-363K shows ...

  5. In Situ Solid Particle Generator

    Science.gov (United States)

    Agui, Juan H.; Vijayakumar, R.

    2013-01-01

    Particle seeding is a key diagnostic component of filter testing and flow imaging techniques. Typical particle generators rely on pressurized air or gas sources to propel the particles into the flow field. Other techniques involve liquid droplet atomizers. These conventional techniques have drawbacks that include challenging access to the flow field, flow and pressure disturbances to the investigated flow, and they are prohibitive in high-temperature, non-standard, extreme, and closed-system flow conditions and environments. In this concept, the particles are supplied directly within a flow environment. A particle sample cartridge containing the particles is positioned somewhere inside the flow field. The particles are ejected into the flow by mechanical brush/wiper feeding and sieving that takes place within the cartridge chamber. Some aspects of this concept are based on established material handling techniques, but they have not been used previously in the current configuration, in combination with flow seeding concepts, and in the current operational mode. Unlike other particle generation methods, this concept has control over the particle size range ejected, breaks up agglomerates, and is gravity-independent. This makes this device useful for testing in microgravity environments.

  6. Generation of urine iPS cell line from a patient with obsessive-compulsive disorder using a non-integrative method

    Directory of Open Access Journals (Sweden)

    Jaroslaw Sochacki

    2016-07-01

    Full Text Available Urine sample was collected from a 29-year-old male patient with an early onset form of DSM-5 obsessive-compulsive disorder (OCD, comorbid body dysmorphic and excoriation (skin-picking disorders, and a positive family history for OCD. Urine cell line was established and expanded for the reprogramming procedure. Induced pluripotent stem (iPS cells were derived using the integration-free CytoTune®-iPS 2.0 Sendai Reprogramming Kit, which includes Sendai virus particles of the four Yamanaka factors Oct3/4, Sox2, Klf4 and c-Myc.

  7. psRNATarget: a plant small RNA target analysis server.

    Science.gov (United States)

    Dai, Xinbin; Zhao, Patrick Xuechun

    2011-07-01

    Plant endogenous non-coding short small RNAs (20-24 nt), including microRNAs (miRNAs) and a subset of small interfering RNAs (ta-siRNAs), play important role in gene expression regulatory networks (GRNs). For example, many transcription factors and development-related genes have been reported as targets of these regulatory small RNAs. Although a number of miRNA target prediction algorithms and programs have been developed, most of them were designed for animal miRNAs which are significantly different from plant miRNAs in the target recognition process. These differences demand the development of separate plant miRNA (and ta-siRNA) target analysis tool(s). We present psRNATarget, a plant small RNA target analysis server, which features two important analysis functions: (i) reverse complementary matching between small RNA and target transcript using a proven scoring schema, and (ii) target-site accessibility evaluation by calculating unpaired energy (UPE) required to 'open' secondary structure around small RNA's target site on mRNA. The psRNATarget incorporates recent discoveries in plant miRNA target recognition, e.g. it distinguishes translational and post-transcriptional inhibition, and it reports the number of small RNA/target site pairs that may affect small RNA binding activity to target transcript. The psRNATarget server is designed for high-throughput analysis of next-generation data with an efficient distributed computing back-end pipeline that runs on a Linux cluster. The server front-end integrates three simplified user-friendly interfaces to accept user-submitted or preloaded small RNAs and transcript sequences; and outputs a comprehensive list of small RNA/target pairs along with the online tools for batch downloading, key word searching and results sorting. The psRNATarget server is freely available at http://plantgrn.noble.org/psRNATarget/.

  8. Enhancement of lateral growth of the GaN crystal with extremely low dislocation density during the Na-flux growth on a point seed

    Science.gov (United States)

    Hayashi, Masatoshi; Imanishi, Masayuki; Yamada, Takumi; Matsuo, Daisuke; Murakami, Kosuke; Maruyama, Mihoko; Imade, Mamoru; Yoshimura, Masashi; Mori, Yusuke

    2017-06-01

    Recently, we developed a Na-flux point seed technique for fabrication of large-diameter gallium nitride (GaN) crystals with low dislocation density. It may be possible to further reduce the dislocation density of crystals fabricated by this technique by reducing the point-seed (PS) density, because one dislocation remains on the c face of each grain grown on each point seed. Therefore, in order to realize coalescence growth by using a Multi-PS-GaN substrate with a low PS density, namely a long interval between neighboring point seeds, the lateral growth of each grain grown on a point seed must be enhanced. We attempted to promote the lateral growth of GaN crystals grown on a point seed by decreasing the flux thickness. As a result, we realized a high growth rate of 80 μm/h along the a direction and a low aspect ratio by the Na-flux point seed technique. Moreover, almost no dislocations could be found in the lateral growth sector. Consequently, growth in a thin flux is applicable for the coalescence growth technique with low PS density and may be an outstanding approach for reducing the dislocation density in GaN crystals grown by the Na-flux coalescence growth technique.

  9. Role of nanosilica localization on morphology development of HDPE/PS/PMMA immiscible ternary blends

    Directory of Open Access Journals (Sweden)

    Z. Javidi

    2017-05-01

    Full Text Available In this work, we studied the parameters affecting the localization of hydrophobic nanosilica particles and its impact on morphology development of polyethylene/polystyrene/poly (methyl methacrylate (HDPE/PS/PMMA ternary blends, which originally have a thermodynamically preferred core–shell type morphology, by means of a combination of rheology and electron microscopy. An attempt was also made to compare the experimental results with thermodynamic predictions. The ternary blend samples with the same blend ratio but varying in nanosilica loadings were prepared by melt compounding using a laboratory internal mixer. It was demonstrated that the nanosilica localization which could be controlled by the sequence of feeding, would play a significant role in determining the morphology development of the nanofilled ternary blend samples. It was shown that in contrary to thermodynamic prediction of a core shell morphology for the nanofilled samples, the highly enhanced melt elasticity of nanosilica filled polystyrene phase did not allow the PS phase to form a complete encapsulating shell.

  10. SANS study of three-layer micellar particles

    CERN Document Server

    Plestil, J; Kuklin, A I; Cubitt, R

    2002-01-01

    Three-layer nanoparticles were prepared by polymerization of methyl methacrylate (MMA) in aqueous micellar solutions of poly(methyl methacrylate)-block-poly(methacrylic acid) (PMMA-b-PMA) and polystyrene-block-poly(methacrylic acid) (PS-b-PMA). The resulting polymer forms a layer on the core surface of the original micelles. SANS curves were fitted using an ellipsoidal (PMMA/PMMA/PMA) or spherical (PS/PMMA/PMA) model for the particle core. The particle size (for the presented series of the PMMA/PMMA/PMA particles, the core semiaxes ranged from 87 to 187 A and the axis ratio was about 6) can be finely tuned by variation of monomer concentration. Time-resolved SANS experiments were carried out to describe the growth of the PS/PMMA/PMA particles during polymerization. (orig.)

  11. factors affecting particle retention in thermal field-flow fractionation

    African Journals Online (AJOL)

    In this paper, we report a range of factors which affect the retention of colloidal particles in thermal field-flow fractionation (ThFFF). These results are observed among different sizes of polystyrene (PS) latex particles suspended in both aqueous and nonaqueous liquid carriers and very low density lipoproteins in a phosphate ...

  12. Surface Modification of Fine Particle by Plasma Grafting in a Circulating Fluidized Bed Reactor under Reduced Pressure

    Energy Technology Data Exchange (ETDEWEB)

    Park, Sounghee [Woosuk University, Jinchon (Korea, Republic of)

    2015-10-15

    A plasma surface modification of powders has been carried out in a circulating fluidized bed reactor under reduced pressure. Polystyrene (PS) particles treated by plasma are grafted with polyethylene glycol (PEG) on the surface. The virgin, plasma-treated and grafted powders were characterized by DPPH method, FTIR, SEM and contact angle meter. The plasma-treated PS powders have well formed peroxide on the surface, By PEG grafting polymerization, PEG is well grafted and dispersed on the surface of the plasma-treated PS powders. The PEG-g-PS particle was successfully synthesized using the plasma circulating fluidized bed reactor under reduced pressure.

  13. Electrodeposition, characterization, and antibacterial activity of zinc/silver particle composite coatings

    Energy Technology Data Exchange (ETDEWEB)

    Reyes-Vidal, Y.; Suarez-Rojas, R.; Ruiz, C.; Torres, J. [Center of Research and Technological Development in Electrochemistry (CIDETEQ), Parque Tecnológico Sanfandila, Pedro Escobedo, Querétaro, A.P.064, C.P.76703, Querétaro (Mexico); Ţălu, Ştefan [Technical University of Cluj-Napoca, Faculty of Mechanical Engineering, Department of AET, Discipline of Descriptive Geometry and Engineering Graphics, 103-105 B-dul Muncii St., Cluj-Napoca 400641 Cluj (Romania); Méndez, Alia [Centro de Química-ICUAP Benemérita Universidad Autónoma de Puebla, Ciudad Universitaria Puebla, 72530 Puebla (Mexico); Trejo, G., E-mail: gtrejo@cideteq.mx [Center of Research and Technological Development in Electrochemistry (CIDETEQ), Parque Tecnológico Sanfandila, Pedro Escobedo, Querétaro, A.P.064, C.P.76703, Querétaro (Mexico)

    2015-07-01

    Highlights: • Zn/AgPs composites coatings were formed for electrodeposition. • CTAB promotes occlusion of silver particles in the coating. • Zn/AgPs coatings present very good antibacterial activity. - Abstract: Composite coatings consisting of zinc and silver particles (Zn/AgPs) with antibacterial activity were prepared using an electrodeposition technique. The morphology, composition, and structure of the Zn/AgPs composite coatings were analyzed using scanning electron microscopy (SEM) coupled with energy-dispersive spectroscopy (EDS), inductively coupled plasma (ICP) spectrometry, and X-ray diffraction (XRD). The antibacterial properties of the coatings against the microorganisms Escherichia coli as a model Gram-negative bacterium and Staphylococcus aureus as a model Gram-positive bacterium were studied quantitatively and qualitatively. The results revealed that the dispersant cetyltrimethylammonium bromide (CTAB) assisted in the formation of a stable suspension of Ag particles in the electrolytic bath for 24 h. Likewise, a high concentration of CTAB in the electrolytic bath promoted an increase in the number of Ag particles occluded in the Zn/AgPs coatings. The Zn/AgPs coatings that were obtained were compact, smooth, and shiny materials. Antimicrobial tests performed on the Zn/AgPs coatings revealed that the inhibition of bacterial growth after 30 min of contact time was between 91% and 98% when the AgPs content ranged from 4.3 to 14.0 mg cm{sup −3}.

  14. A 41 ps ASIC time-to-digital converter for physics experiments

    Science.gov (United States)

    Russo, Stefano; Petra, Nicola; De Caro, Davide; Barbarino, Giancarlo; Strollo, Antonio G. M.

    2011-12-01

    We present a novel Time-to-Digital (TDC) converter for physics experiments. Proposed TDC is based on a synchronous counter and an asynchronous fine interpolator. The fine part of the measurement is obtained using NORA inverters that provide improved resolution. A prototype IC was fabricated in 180 nm CMOS technology. Experimental measurements show that proposed TDC features 41 ps resolution associated with 0.35LSB differential non-linearity, 0.77LSB integral non-linearity and a negligible single shot precision. The whole dynamic range is equal to 18 μs. The proposed TDC is designed using a flash architecture that reduces dead time. Data reported in the paper show that our design is well suited for present and future particle physics experiments.

  15. The Merit(nTOF-11) High Intensity Liquid Mercury Target Experiment at the CERN PS

    CERN Document Server

    Efthymiopoulos, I; Caretta, O; Carroll, A J; Fabich, A; Graves, V B; Grudiev, A; Haug, F; Kirk, H G; Lettry, Jacques; Loveridge, P; McDonald, K T; Mokhov, N; Palm, M; Park, H; Pernegger, H; Spampinato, P T; Steerenberg, R; Striganov, S; Tsang, T

    2008-01-01

    The MERIT(nTOF-11) experiment is a proof-ofprinciple test of a target system for a high power proton beam to be used as front-end for a neutrino factory or a muon collider. The experiment took data in autumn 2007 with the fast-extracted beam from the CERN Proton Synchrotron (PS) to a maximum intensity of $30 × 10^{12}$ per pulse. The target system, based on a free mercury jet, is capable of intercepting a 4-MW proton beam inside a 15-T magnetic field required to capture the low energy secondary pions as the source for intense muon beams. Partice detectors installed around the target setup measure the secondary particle flux out of the target and can probe cavitation effects in the mercury jet when excited by an intense proton beam.Preliminary results of the data analysis will be presented here.

  16. [Retinal Cell Therapy Using iPS Cells].

    Science.gov (United States)

    Takahashi, Masayo

    2016-03-01

    Progress in basic research, starting with the work on neural stem cells in the middle 1990's to embryonic stem (ES) cells and induced pluripotent stem (iPS) cells at present, will lead the cell therapy (regenerative medicine) of various organs, including the central nervous system to a big medical field in the future. The author's group transplanted iPS cell-derived retinal pigment epithelial (RPE) cell sheets to the eye of a patient with exudative age-related macular degeneration (AMD) in 2014 as a clinical research. Replacement of the RPE with the patient's own iPS cell-derived young healthy cell sheet will be one new radical treatment of AMD that is caused by cellular senescence of RPE cells. Since it was the first clinical study using iPS cell-derived cells, the primary endpoint was safety judged by the outcome one year after surgery. The safety of the cell sheet has been confirmed by repeated tumorigenisity tests using immunodeficient mice, as well as purity of the cells, karyotype and genetic analysis. It is, however, also necessary to prove the safety by clinical studies. Following this start, a good strategy considering cost and benefit is needed to make regenerative medicine a standard treatment in the future. Scientifically, the best choice is the autologous RPE cell sheet, but autologous cell are expensive and sheet transplantation involves a risky part of surgical procedure. We should consider human leukocyte antigen (HLA) matched allogeneic transplantation using the HLA 6 loci homozyous iPS cell stock that Prof. Yamanaka of Kyoto University is working on. As the required forms of donor cells will be different depending on types and stages of the target diseases, regenerative medicine will be accomplished in a totally different manner from the present small molecule drugs. Proof of concept (POC) of photoreceptor transplantation in mouse is close to being accomplished using iPS cell-derived photoreceptor cells. The shortest possible course for treatment

  17. A transmissão psíquica geracional

    Directory of Open Access Journals (Sweden)

    Vinícius Oliveira dos Santos

    Full Text Available O artigo seguinte refere-se a um estudo sobre como ocorre a transmissão psíquica entre as gerações e qual sua importância na constituição psíquica do sujeito. É também objetivo deste artigo explicar o que são as transmissões intergeracional e transgeracional. Para buscar respostas para essas questões, fez-se uma pesquisa bibliográfica sobre a transmissão psíquica, pelo viés psicanalítico, principalmente a partir da teoria lacaniana e com conceitos oriundos da linguística saussuriana. Será a partir de uma determinada ordem simbólica, constituída pela linguagem que precede o sujeito, nomeado por Lacan como o Outro, que a transmissão psíquica entre gerações ganhará o seu caráter unívoco, sempre se tendo em mente a importância fundamental do recalcamento e de seus efeitos, bem como do retorno do recalcado nas diferentes gerações. A transmissão psíquica é necessária e concomitante à constituição do sujeito, e ocorre através da linguagem, dos significantes que irão determinar uma ordem simbólica para o ser que nasce através dos diferentes discursos que perpassam as gerações nas figuras dos pais desse novo ser. Essa ordem simbólica continuará a se fazer presente nesse novo sujeito pelo restante de sua existência. Este artigo busca dar nova luz ao aspecto da transmissão psíquica transgeracional, diferenciando-se da recalque s abordagens psicanalíticas contemporâneas por ser uma leitura lacaniana. Serão usados dois exemplos: um de como a transmissão aparece na cultura, outro, na subjetividade do sujeito através da arte.

  18. Oil palm seed distribution

    Directory of Open Access Journals (Sweden)

    Durand-Gasselin Tristan

    2005-03-01

    Full Text Available For a tropical plant, the oil palm commodity chain has the peculiarity of possessing a major seed production sector for reasons that are primarily genetic. This seed sector has numerous original aspects. Breeders are also propagators and usually also distribute their seeds. Oil palm seeds are semi-recalcitrant: they display pseudo-dormancy. Achieving seed germination is difficult and requires lengthy treatments and special installations. This restriction greatly influences seed distribution and the role of the different stakeholders in the commodity chain. It was only once it had been discovered how the “sh” gene functioned, which controls shell thickness, and when it became necessary to produce “tenera” seeds derived from exclusively “dura x pisifera” crosses, that a true seed market developed. In addition it is difficult to organize seed distribution to smallholders. This is partly due to difficulties that the profession, or a State-run organization, has in controlling middlemen networks, and partly to the absence of any protective systems (UPOV, plant breeder certificate, etc. that generally oblige breeders to preserve and propagate parents in their own installations. In fact there are major inequalities in the access to seeds between agroindustry and smallholders. Another peculiarity of the oil palm seed market is the virtually total absence of guarantees for buyers: the quality of the research conducted by breeders, the seed production strategies necessary for transferring genetic progress, and the technical quality of production. The only guarantee today comes from the relations of confidence established year after year between breeders/distributors and growers. In this fields, research can lead to some proposals: molecular biology offers some interesting prospects for certifying seed quality and social science develop effective communication methods.

  19. Does the informal seed system threaten cowpea seed health?

    NARCIS (Netherlands)

    Biemond, P.C.; Oguntade, O.; Lava Kumar, P.; Stomph, T.J.; Termorshuizen, A.J.; Struik, P.C.

    2013-01-01

    Most smallholder farmers in developing countries depend on an informal Seed System (SS) for their seed. The informal SS is often criticized because farmer-produced seed samples are not tested for seed health, thus accepting the risk of planting infected seeds. Here we aimed at assessing the quality

  20. Effect of seed invigoration by osmopriming on seed quality ...

    African Journals Online (AJOL)

    Slow and uneven germination of seeds of Capsicum species are undesirable to the seed producers and farmers. Seed osmo priming is one of the pre-treatments for enhancing seed quality. An experiment was conducted to determine the influence of osmopriming techniques on seed lots of three Capsicum species.

  1. The influence of some factors on the electrical conductivity and particle size of core/shell polystyrene/polyaniline composites

    Directory of Open Access Journals (Sweden)

    GORDANA D. NESTOROVIC

    2005-11-01

    Full Text Available The electrically conductive, micron-sized, core/shell polystyrene (PS/polyaniline (PANI composite particles were synthesized by chemical oxidative polymerization of aniline in the presence of micron-sized PS particles in 1 M HCl. The conditions of the dispersion polymerization of styrene were optimized. The influence of the initiator type employed for the chemical oxidative polymerization of aniline and the aniline (ANI concentration on the PS/PANI particle size and size distribution and their conductivity was investigated. The obtained results show that the conductivity of the samples increased with increasing ANI concentration. The conductivity of the PS/PANI composite particles obtained with the highest ANI concentration was of the same order of magnitude as that for PANI powder. The particle size did not depend on the concentration of ANI, while the particle size distribution was narrower at higher concentrations of ANI.

  2. Direct observation of new particle formation during ozonolysis of isoprene and ethene competing against the growth of preexisting particles

    Science.gov (United States)

    Inomata, Satoshi; Sato, Kei; Sakamoto, Yosuke; Hirokawa, Jun

    2017-12-01

    Secondary organic aerosol formation during the ozonolysis of isoprene and ethene in the presence of ammonium nitrate seed particles (surface area concentrations = (0.8-3) × 107 nm2 cm-3) was investigated using a 1 nm scanning mobility particle sizer. Based on the size distribution of formed particles, particles with a diameter smaller than the minimum diameter of the seed particles (less than ∼6 nm) formed under dry conditions, but the formation of such particles was substantially suppressed during isoprene ozonolysis and was not observed during ethane ozonolysis under humid conditions. We propose that oligomeric hydroperoxides generated by stabilized Criegee intermediates (sCIs), including C1-sCI (CH2OO), contribute to new particle formation while competing to be taken up onto preexisting particles. The OH reaction products of isoprene and ethene seem to not contribute to new particle formation; however, they are taken up onto preexisting particles and contribute to particle growth.

  3. Transfer line from the PSB to the PS (recombination)

    CERN Multimedia

    CERN PhotoLab

    1976-01-01

    After sequential ejection of 5 bunches from each of the 4 rings of the Booster (originally 800 MeV, now 1.4 GeV), the 4 batches are brought to the same vertical level, so as to form a string of 20 bunches, filling the circumference of the PS. This vertical "recombination" is performed in the transfer line, using vertical bending magnets, septa and kickers. Here we see the section where the beam from ring 4 (the top one) is brought down to the level of ring 3, and the beam from ring 1 up to the level of ring 2. Further downstream (to the right, outside this picture), level 2 is brought up to level 3, identical to that of the PS. After this original recombination scheme, other ways of combining the 4 beams, vertically and/or longitudinally, were developed and used in operation.

  4. O Trabalho Psíquico da Intersubjetividade

    Directory of Open Access Journals (Sweden)

    Maria Inês Assumpção Fernandes

    2003-01-01

    Full Text Available O presente trabalho procura refletir sobre o trabalho psíquico da intersubjetividade nos grupos. Trata-se de pensá-lo na relação com a ruptura de investimentos durante o processo de Transformação x Criação, em primeiro lugar. A partir desse ponto, discutiremos a relação entre Transformação, Trabalho e Dispositivo. Neste caso pensamos nas possibilidades de intervenção, refletindo sobre a intervenção inpidual e a intervenção grupal. A questão da Transmissão Psíquica entre gerações será focalizada, fundamentalmente, no que se refere aos tempos lógicos do recalque.

  5. Images of Christ's Saving Work in Ps.-Epiphanius' Homilies

    Directory of Open Access Journals (Sweden)

    H. F. Stander

    1997-12-01

    Full Text Available Images of Christ's Saving Work in Ps.-Epiphanius' Homilies. One cannot really speak of a systematic theology on the subject of atone-ment in the patristic writers. Frances Young once said that 'it is in fact impossible to categorize neatly the thought of the major patristic writers on the subject of atonement'. She adds that one cannot do justice to the range of motifs and images that are found in describing the saving and atoning work of Christ if we merely dismember 'systematic theologies' to illustrate common soteriological themes. One can only appreciate patristic views of atonement if one begins by recognizing the multifaceted unity of imagery that pervades the literature. This then is the goal of this article: to discuss the rich images which Ps: -Epiphanius uses to describe the atoning work of Christ.

  6. Magnetoelectric MnPS3 as a candidate for ferrotoroidicity

    Science.gov (United States)

    Ressouche, E.; Loire, M.; Simonet, V.; Ballou, R.; Stunault, A.; Wildes, A.

    2010-09-01

    We have revisited the magnetic structure of manganese phosphorus trisulfide MnPS3 using neutron diffraction and polarimetry. MnPS3 undergoes a transition toward a collinear antiferromagnetic order at 78 K. The resulting magnetic point-group breaks both the time reversal and the space inversion thus allowing a linear magnetoelectric coupling. Neutron polarimetry was subsequently used to prove that this coupling provides a way to manipulate the antiferromagnetic domains simply by cooling the sample under crossed magnetic and electrical fields, in agreement with the nondiagonal form of the magnetoelectric tensor. In addition, this tensor has, in principle, an antisymmetric part that results in a toroidic moment and provides with a pure ferrotoroidic compound.

  7. PS Dreyer: Bakens op die pad van die wetenskap

    Directory of Open Access Journals (Sweden)

    A. J. Antonites

    1986-01-01

    Full Text Available PS Dreyer: Beacons on the path of science Professor PS Dreyer is an academic who has shown insight and vision into several problems of the human sciences since 1951. He has identified problems, but also contributed solutions to them. In this respect his philosophy on causality and freedom is of utmost importance. The same applies to his investigations into the relationship history-Christianity as well as the unity of sciences and how the concepts scientific, unscientic and nonscientific are related to one another. His contribution to the understanding of Greek philosophy should be of significance for time to come. Two milestones could be distinguished: Dreyer's particular solution to the problem of the criterion on truth, viz meaningfulness and his notion of the knowledge of values in ethics by valuation in contradistinction to knowledge through feeling, reason and will.

  8. Images of Christ's Saving Work in Ps.-Epiphanius' Homilies

    Directory of Open Access Journals (Sweden)

    H. F. Stander

    1997-01-01

    Full Text Available Images of Christ's Saving Work in Ps.-Epiphanius' Homilies. One cannot really speak of a systematic theology on the subject of atone-ment in the patristic writers. Frances Young once said that 'it is in fact impossible to categorize neatly the thought of the major patristic writers on the subject of atonement'. She adds that one cannot do justice to the range of motifs and images that are found in describing the saving and atoning work of Christ if we merely dismember 'systematic theologies' to illustrate common soteriological themes. One can only appreciate patristic views of atonement if one begins by recognizing the multifaceted unity of imagery that pervades the literature. This then is the goal of this article: to discuss the rich images which Ps: -Epiphanius uses to describe the atoning work of Christ.

  9. Longitudinal coupled-bunch instability studies in the PS

    CERN Document Server

    Damerau, H

    2017-01-01

    The main longitudinal limitation for LHC-type beams inthe PS are coupled-bunch instabilities. A dedicated proto-typefeedbacksystemusingaFinemetcavityasalongitudinalkicker has been installed. Extensive tests with beam havebeen performed to explore the intensity reach with this feed-back. The maximum intensity with nominal longitudinalemittance at PS extraction has been measured, as well as theemittance required to keep the beam longitudinally stableat the design intensity for the High-Luminosity LHC (HL-LHC). A higher-harmonic cavity is a complementary op-tion to extend the intensity reach beyond the capabilities ofthe coupled-bunch feedback. Preliminary machine develop-ment (MD) studies operating one20MHzor one40MHzRF system as a higher harmonic at the flat-top indicate thebeneficial effect on longitudinal beam stability

  10. Particle Characterization for a Protein Drug Product Stored in Pre-Filled Syringes Using Micro-Flow Imaging, Archimedes, and Quartz Crystal Microbalance with Dissipation.

    Science.gov (United States)

    Zheng, Songyan; Puri, Aastha; Li, Jinjiang; Jaiswal, Archana; Adams, Monica

    2017-01-01

    Micro-flow imaging (MFI) has been used for formulation development for analyzing sub-visible particles. Archimedes, a novel technique for analyzing sub-micron particles, has been considered as an orthogonal method to currently existing techniques. This study utilized these two techniques to investigate the effectiveness of polysorbate (PS-80) in mitigating the particle formation of a therapeutic protein formulation stored in silicone oil-coated pre-filled syringes. The results indicated that PS-80 prevented the formation of both protein and silicone oil particles. In the case of protein particles, PS-80 might involve in the interactions with the hydrophobic patches of protein, air bubbles, and the stressed surfaces of silicone oil-coated pre-filled syringes. Such interactions played a role in mitigating the formation of protein particles. Subsequently, quartz crystal microbalance with dissipation (QCM-D) was utilized to characterize the interactions associated with silicone oil, protein, and PS-80 in the solutions. Based on QCM-D results, we proposed that PS-80 likely formed a layer on the interior surfaces of syringes. As a result, the adsorbed PS-80 might block the leakage of silicone oil from the surfaces to solution so that the silicone oil particles were mitigated at the presence of PS-80. Overall, this study demonstrated the necessary of utilizing these three techniques cooperatively in order to better understand the interfacial role of PS-80 in mitigating the formation of protein and silicone oil particles.

  11. Functional characterization of calcineurin homologs PsCNA1/PsCNB1 in Puccinia striiformis f. sp. tritici using a host-induced RNAi system.

    Directory of Open Access Journals (Sweden)

    Hong Zhang

    Full Text Available Calcineurin plays a key role in morphogenesis, pathogenesis and drug resistance in most fungi. However, the function of calcineurin genes in Puccinia striiformis f. sp. tritici (Pst is unclear. We identified and characterized the calcineurin genes PsCNA1 and PsCNB1 in Pst. Phylogenetic analyses indicate that PsCNA1 and PsCNB1 form a calcium/calmodulin regulated protein phosphatase belonging to the calcineurin heterodimers composed of subunits A and B. Quantitative RT-PCR analyses revealed that both PsCNA1 and PsCNB1 expression reached their maximum in the stage of haustorium formation, which is one day after inoculation. Using barely stripe mosaic virus (BSMV as a transient expression vector in wheat, the expression of PsCNA1 and PsCNB1 in Pst was suppressed, leading to slower extension of fungal hyphae and reduced production of urediospores. The immune-suppressive drugs cyclosporin A and FK506 markedly reduced the germination rates of urediospores, and when germination did occur, more than two germtubes were produced. These results suggest that the calcineurin signaling pathway participates in stripe rust morphogenetic differentiation, especially the formation of haustoria during the early stage of infection and during the production of urediospores. Therefore PsCNA1 and PsCNB1 can be considered important pathogenicity genes involved in the wheat-Pst interaction.

  12. Consolidation of the 45-Year Old PS Main Magnet System

    CERN Document Server

    Zickler, Thomas; Kalbreier, Wilhelm; Mess, Karl Hubert; Newborough, Antony

    2006-01-01

    After a major coil insulation breakdown on two of the 47-year-old CERN PS main magnets in 2003, an extensive magnet consolidation program has been launched. This article reviews the analysis of the magnet state be-fore the repair and the applied major improvements. An overview is given of the production of the new compo-nents, the actual refurbishment and the commissioning of the main magnet system after 18 months shutdown.

  13. Specification of the Beam Position Measurement in the PS Machine

    CERN Document Server

    Bravin, Enrico; Chanel, M; Ludwig, M; Métral, Elias; Métral, G; Potier, J P; Raich, U; Scrivens, R; Steerenberg, R; CERN. Geneva. AB Department

    2003-01-01

    This specification, drawn up by the instrumentation specification board 2, describes the requirements concerning orbit and trajectory measurements in the PS machine. The orbit measurement and the trajectory measurement are both indispensable in order to be able to guarantee the correct beam quality for beams like LHC, the future Grand Sasso beam, the nTOF beam and surely the combined operation of the nTOF beam and the East Area beam.

  14. Science spin: iPS cell research in the news.

    Science.gov (United States)

    Caulfield, T; Rachul, C

    2011-05-01

    Big scientific developments have always been spun to meet particular social agendas. We have seen it in the context of global warming, nuclear power, and genetically modified organisms. But few stories illustrate the phenomenon of spin as well as the reaction, and concomitant media coverage, that surrounded the November 2007 announcement regarding the reprogramming of skin cells to produce cells with qualities comparable to those of human embryonic stem cells (hESCs) known as induced pluripotent stem (iPS) cells.

  15. Physics at the AD/PS/SPS (1/4)

    CERN Multimedia

    CERN. Geneva

    2012-01-01

    Lecture 1: The CERN injector complex and beams for non-LHC physics. The various machines and beam lines in the CERN injector complex are presented, from the linacs to the SPS. Special emphasis is given to the beam lines at the PS and SPS machines: AD, North and East Areas, nTOF and CNGS and HiRadMad as well as the ion beams. A short outlook is given to possible future upgrades and projects.

  16. Ps18.pdf | sep2002 | jess | Indian Academy of Sciences

    Indian Academy of Sciences (India)

    Home; jess; sep2002; Ps18.pdf. 404! error. The page your are looking for can not be found! Please check the link or use the navigation bar at the top. YouTube; Twitter; Facebook; Blog. Academy News. IAS Logo. Associates – 2017. Posted on 17 July 2017. Click here to see the list · 28th Mid Year Meeting. Posted on 26 May ...

  17. Hexagonal-to-cubic phase transformation in composite thin films induced by FePt nanoparticles located at PS/PEO interfaces.

    Science.gov (United States)

    Aissou, Karim; Fleury, Guillaume; Pecastaings, Gilles; Alnasser, Thomas; Mornet, Stéphane; Goglio, Graziella; Hadziioannou, Georges

    2011-12-06

    The organization process of asymmetric poly(styrene-block-ethylene oxide) (PS-b-PEO) copolymer thin films blended with FePt nanoparticles is studied. In a first step, it is shown that FePt nanoparticles stabilized by oleic acid ligands are distributed within the PS matrix phase, whereas the same particles partially covered with short dopamine-terminated-methoxy poly(ethylene oxide) (mPEO-Dopa) are located at PS/PEO interfaces. The swelling of PS domains, induced by FePt_oleic acid nanoparticles during the solvent annealing process, results in formation of a disordered microstructure in comparison to the well-organized hexagonally close-packed (HCP) cylinder phase formed in the neat PS-b-PEO copolymer. The evolution of the microstructure of PS-b-PEO/FePt_mPEO-Dopa composite has been investigated for different solvent annealing treatments. Under high-humidity conditions during the vapor annealing process, the addition of FePt nanoparticles results in formation of spheres in the film split into terraces. The upper and lower terraces are occupied by spheres organized in an unusual square and HCP phases, respectively. Under low-humidity conditions, undulated PEO cylinders oriented parallel to substrate are formed in the presence of FePt nanoparticles. In this case, we observe that most of the nanoparticles accumulate within the core of topological defects, which induces a low nanoparticle concentration at the PS/PEO interfaces and so stabilizes an intermediate undulated cylinder phase. © 2011 American Chemical Society

  18. Sesame seed lignans

    OpenAIRE

    Moazzami, Ali

    2006-01-01

    Sesame seed has been an important oilseed since ancient times. It contains 25% protein and 50% oil. Sesame seed possesses many health promoting effects, some of which have been attributed to a group of compounds called lignans (sesamin, sesamolin, sesaminol and sesamolinol). Sesame seed contains lignan aglycones in oil and lignan glucosides. Sesamin, the major sesame oil lignan, is known to reduce plasma cholesterol and to increase plasma γ-tocopherol in humans. This thesis aimed at investiga...

  19. New Electron Cloud Detectors for the PS Main Magnets

    CERN Document Server

    Yin Vallgren, Ch; Gilardoni, S; Taborelli, M; Neupert, H; Ferreira Somoza, J

    2014-01-01

    Electron cloud (EC) has already been observed during normal operation of the PS, therefore it is necessary to study its in fluence on any beam instability for the future LHC Injector Upgrade (LIU). Two new electron cloud detectors have been discussed, developed and installed during the Long Shutdown (LS1) in one of the PS main magnets. The first measurement method is based on current measurement by using a shielded button-type pick-up. Due to the geometry and space limitation in the PS magnet, the button-type pick-up made of a 96%Al2O3 block coated with a thin layer of solvent-based Ag painting, placed 30 degrees to the bottom part of the vacuum chamber was installed in the horizontal direction where the only opening of the magnet coil is. The other newly developed measurement method is based on detection of photons emitted by the electrons from the electron cloud impinging on the vacuum chamber walls. The emitted photons are reected to a quartz window. A MCP-PMT (Micro-Channel Plate Photomultiplier Tube) wit...

  20. LS1 Report: PS Booster prepares for beam

    CERN Multimedia

    Katarina Anthony

    2014-01-01

    With Linac2 already up and running, the countdown to beam in the LHC has begun! The next in line is the PS Booster, which will close up shop to engineers early next week. The injector will be handed over to the Operations Group who are tasked with getting it ready for active duty.   Taken as we approach the end of LS1 activities, this image shows where protons will soon be injected from Linac2 into the four PS Booster rings. Over the coming two months, the Operations Group will be putting the Booster's new elements through their paces. "Because of the wide range of upgrades and repairs carried out in the Booster, we have a very full schedule of tests planned for the machine," says Bettina Mikulec, PS Booster Engineer in Charge. "We will begin with cold checks; these are a wide range of tests carried out without beam, including system tests with power on/off and with varying settings, as well as verification of the controls system and timings." Amon...

  1. Polygamous particles

    OpenAIRE

    Wu, Kun-Ta; Feng, Lang; Sha, Ruojie; Dreyfus, Rémi; Grosberg, Alexander Y.; Seeman, Nadrian C.; Chaikin, Paul M.

    2012-01-01

    DNA is increasingly used as an important tool in programming the self-assembly of micrometer- and nanometer-scale particles. This is largely due to the highly specific thermoreversible interaction of cDNA strands, which, when placed on different particles, have been used to bind precise pairs in aggregates and crystals. However, DNA functionalized particles will only reach their true potential for particle assembly when each particle can address and bind to many different kinds of particles. ...

  2. Lifetime measurements of the D +, D 0, D {/s +}, and Λ {/c +} charmed particles

    Science.gov (United States)

    Alvarez, M. P.; Barate, R.; Bloch, D.; Bonamy, P.; Borgeaud, P.; Burchell, M.; Burmeister, H.; Brunet, J. M.; Calvino, F.; Cattaneo, M.; Crespo, J. M.; D'Almagne, B.; David, M.; Diciaccio, L.; Dixon, J.; Druet, P.; Duane, A.; Engel, J. P.; Ferrer, A.; Filippas, T. A.; Fokitis, E.; Forty, R. W.; Foucault, P.; Gazis, E. N.; Gerber, J. P.; Giomataris, Y.; Hofmokl, T.; Katsoutis, E. C.; Koratzinos, M.; Krafft, C.; Lefievre, B.; Lemoigne, Y.; Lopez, A.; Lui, W. K.; Magneville, C.; Maltezos, A.; McEwen, J. G.; Papadopoulou, Th.; Pattison, B.; Poutot, D.; Primout, M.; Rahmani, H.; Roudeau, P.; Seez, C.; Six, J.; Strub, R.; Treille, D.; Triscos, P.; Tristram, G.; Villet, G.; Volte, A.; Wayne, M.; Websdale, D. M.; Wormser, G.; Zolnierowski, Y.

    1990-12-01

    Using a high-statistics sample of more than 1000 reconstructed charmed-particle decays, the lifetimes of the weakly decaying charmed mesons and of the Λ {/c +} baryon have been measured: 10052_2005_Article_BF01552318_TeX2GIFE1.gif begin{gathered} tau _{D^ + } = 1.03 ± 0.08 + 0.06 ps,tau _{D^0 } = 0.417 ± 0.018 ± 0.015 ps \\ tau _{D_s^ + } = 0.33_{ - 0.08}^{ + 0.12} ± 0.03 ps,tau _{Λ _c^ + } = 0.18 ± 0.03 ± 0.03 ps. \\

  3. Particle Strength of Bayer Hydrate

    Science.gov (United States)

    Anjier, J. L.; Marten, D. F. G.

    Because of the proposed use of fluid bed calciners at the Kaiser Aluminum Baton Rouge Works, studies into the strength of alumina and alumina trihydrate from eight different alumina plants were initiated. It was found in the course of these studies that the particle strength of Bayer hydrate depended on the precipitation process conditions under which it was produced. A series of laboratory precipitation tests was conducted to determine the effect on particle strength of process variables such as seed charge, temperature, caustic concentration and seed recycle. It is concluded from these studies that relative particle strength of alumina trihydrate, as measured by a modified Forsythe-Hertwig Apparatus, can be predicted from a knowledge of the precipitation process conditions.

  4. Tuning particle geometry of chemically anisotropic dumbbell-shaped colloids.

    Science.gov (United States)

    van Ravensteijn, Bas G P; Kegel, Willem K

    2017-03-15

    Chemically anisotropic dumbbell-shaped colloids are prepared starting from cross-linked polymer seed particles coated with a chlorinated outer layer. These chlorinated seeds are swollen with monomer. Subsequently, a liquid protrusion is formed on the surface of the seed particle by phase separation between the monomer and the swollen polymer network. Solidification of these liquid lobes by polymerization leads to the desired dumbbell-shaped colloids. The chlorine groups remain confined on the seed lobe of the particles, ensuring chemical anisotropy of the resulting particles. Exploiting the asymmetric distribution of the chemically reactive surface chlorine groups allows for site-specific surface modifications. Here we show that the geometry of the resulting chemically anisotropic dumbbells can be systematically tuned by a number of experimental parameters including the volume of styrene by which the seeds are swollen, the cross-link density of the chlorinated seeds and chemical composition/thickness of the chlorinated coating deposited on the seed particles. Being able to control the particle geometry, and therefore the Janus balance of these chemically anisotropic particles, provides a promising starting point for the synthesis of sophisticated building blocks for future (self-assembly) studies. Copyright © 2016 Elsevier Inc. All rights reserved.

  5. Kultuur isiksuse psühholoogiat ei mõjuta / Tiit Kändler

    Index Scriptorium Estoniae

    Kändler, Tiit, 1948-

    2010-01-01

    Psühholoogia uuemate andmete kohaselt ei sõltu indiviidi seadumus kultuurist, soost, vanusest, haridusest. Eesti psühholoogide Jüri Alliku ja Ann Realo osalusel ajakirjas "Journal Personality and Social Psychology" ilmunud artiklist

  6. Kultuur isiksuse psühholoogiat ei mõjuta / Tiit Kändler

    Index Scriptorium Estoniae

    Kändler, Tiit, 1948-

    2005-01-01

    Psühholoogia uuemate andmete kohaselt ei sõltu indiviidi seadumus kultuurist, soost, vanusest, haridusest. Eesti psühholoogide Jüri Alliku ja Anu Realo osalusel ajakirjas "Journal Personality and Social Psychology" ilmunud artiklist

  7. Comparative, validity and responsiveness of the HOOS-PS and KOOS-PS to the WOMAC physical function subscale in total joint replacement for osteoarthritis

    DEFF Research Database (Denmark)

    Davis, A M; Perruccio, A V; Canizares, M

    2009-01-01

    OBJECTIVE: To evaluate the internal consistency of the Hip disability and Osteoarthritis Outcome Score-Physical Function Short-form (HOOS-PS) and the Knee injury and Osteoarthritis Outcome Score-Physical Function Short-form (KOOS-PS) in total hip replacement (THR) and total knee (TKR) replacement....... Construct validity and responsiveness were compared to the Western Ontario McMaster Universities' Osteoarthritis Index (WOMAC) Likert 3.0 physical function (PF) subscale and the PF excluding the items in the short measures (PF-exclusions). METHODS: Participants completed the full HOOS or KOOS, measures...... of fatigue, anxiety, depression and the Chronic Pain Grade (CPG) pre-surgery and the HOOS or KOOS 6 months post-surgery. Internal consistency for the HOOS-PS and KOOS-PS was calculated using Cronbach's alpha. For construct validity, it was hypothesized that correlations between the HOOS-PS or KOOS-PS and PF...

  8. Paper (essay on seed

    Directory of Open Access Journals (Sweden)

    Mirić Mladen

    2013-01-01

    Full Text Available Based on detailed studies of the past of the agrarian thought of the world (evolution of agro-innovation, and within it, the relationship of man and seed, the author has selected key data for Table 1. In addition, more or less known folk sayings, proverbs, curses and allegories in which the seed is the key word have been collected. Then, religious books, folk art and literature works (sayings of prominent individuals and experts and observations of the author himself have been studied. According to the collected opus, it can be concluded that the vast importance of seed meaningfully entwined into all spheres of folk life and cultural heritage. Seed is directly tied to the following eight key (revolutionary milestones in the food and economic life of mankind: 1. the first and for the longest time, the seed used to be the main food of the people and the first food reserve; 2. Neolithic Revolution was simply caused by the sowing of seeds; 3. for the sake of sowing man began to develop more complex processing techniques; 4. everywhere and forever, especially since the late 15th century, the seed has been a carrier of (rescuing plants between Europe and the New World, that is, between continents; 5. seed was the first product that has been chemically treated since the mid-18th century; 6. standard operation procedures and quality are promoted on seed by which it became the first product to have prescribed (compulsory methods, but it also became a good whose quality has to be tested before sale; 7. hybrid seed is a 'perpetrator' of the green revolution in the mid-20th century and at last there is disputable seed of genetically modified organisms that are spreading with certain reactions. The author proposes that the United Nations Standard International Trade Classification includes a special section (division which would classify the seed for sowing, while beyond this Classification terms such as seed, plant seed should be replaced with non-seed

  9. Seed dormancy and germination.

    Science.gov (United States)

    Penfield, Steven

    2017-09-11

    Reproduction is a critical time in plant life history. Therefore, genes affecting seed dormancy and germination are among those under strongest selection in natural plant populations. Germination terminates seed dispersal and thus influences the location and timing of plant growth. After seed shedding, germination can be prevented by a property known as seed dormancy. In practise, seeds are rarely either dormant or non-dormant, but seeds whose dormancy-inducing pathways are activated to higher levels will germinate in an ever-narrower range of environments. Thus, measurements of dormancy must always be accompanied by analysis of environmental contexts in which phenotypes or behaviours are described. At its simplest, dormancy can be imposed by the formation of a simple physical barrier around the seed through which gas exchange and the passage of water are prevented. Seeds featuring this so-called 'physical dormancy' often require either scarification or passage through an animal gut (replete with its associated digestive enzymes) to disrupt the barrier and permit germination. In other types of seeds with 'morphological dormancy' the embryo remains under-developed at maturity and a dormant phase exists as the embryo continues its growth post-shedding, eventually breaking through the surrounding tissues. By far, the majority of seeds exhibit 'physiological dormancy' - a quiescence program initiated by either the embryo or the surrounding endosperm tissues. Physiological dormancy uses germination-inhibiting hormones to prevent germination in the absence of the specific environmental triggers that promote germination. During and after germination, early seedling growth is supported by catabolism of stored reserves of protein, oil or starch accumulated during seed maturation. These reserves support cell expansion, chloroplast development and root growth until photoauxotrophic growth can be resumed. Crown Copyright © 2017. Published by Elsevier Ltd. All rights reserved.

  10. Condensation and deposition of seed in the MHD bottoming plant

    Energy Technology Data Exchange (ETDEWEB)

    Im, K. H.; Patten, J.; Johnson, T. R.; Tempelmeyer, K.

    1979-01-01

    The computer models of slag vapor nucleation and particle deposition have been extended to predict the growth and deposition of seed particles in the steam and air heater sections of the MHD bottoming plant. The model represents a hot combustion gas stream, which contains vaporized seed and entrained slag particles of a selected initial size distribution, flowing through a bank of cooled tubes. The energy balance includes convective and radiant heat transfer to the cool surfaces. The material balance for the condensible species considers convective mass transport of seed vapor to cool surfaces, and the deposition of particles on cooled surfaces by thermophoresis. The analyses provide the bases for design trade-off studies of steam tube size and spacing, gas velocity, and system configuration to optimize the effectiveness and cost of the steam plant. In the absence of entrained slag particles, sample calculations indicated that, as the gas is cooled in passing through a tube bank, the bulk of the seed vapor condenses in the gas stream to form particles with diameters in the range of 0.02 to 0.2 ..mu..m. In the presence of the submicron slag particles formed upstream in the MHD diffuser, the largest fraction of the seed vapor condenses on the existing entrained particles, causing them to grow to a size in the range of approximately one micron. In both cases, these particles are deposited on heat exchange surfaces throughout the heat recovery system and a large fraction is present in the cool combustion gas entering the exhaust gas clean-up system.

  11. Analüütilised voolud psühholoogias ja nende rakendamine pedagoogikas / Aleksander Elango

    Index Scriptorium Estoniae

    Elango, Aleksander, 1902-2004

    2001-01-01

    Analüütise psühholoogia kolm koolkonda - S.Freudì koolkond e. päris-psühhoanalüüs, A.Adlerì koolkond e. individuaalpsühholoogia ja C.G.Jungì psühhoanalüüsi ja individuaalpsühholoogia sünteesi luua püüdev koolkond. Analüütise psühholoogia koolkondade ja pedagoogika suhetest

  12. Enhancing efficacy and mucosa-tropic distribution of an oral HIV-PsV DNA vaccine in animal models.

    Science.gov (United States)

    Fu, Jie; Bian, Guangxing; Zhao, Bijun; Dong, Zengxiang; Sun, Xiao; Chen, Fang; Ou, Lun; Song, Haifeng

    2009-12-01

    A strategy combined the oral delivery route and bovine papillomavirus (BPV) pseudovirus (PsV)-based human immunodeficiency virus (HIV) DNA vaccine, which has been proven to enhance the mucosal immunization compared with the systemic immunization and in general does not induce effective mucosal immune responses. In this study, the immune responses against the BPV expressing HIV gp41 epitopes (ELDKWA, NWFDIT) after oral administration in Cynomolgus monkeys (Macaca fascicularis) were assessed, and the biodistribution of plasmid DNA encapsulated in the papillomavirus-like particles (VLPs) were evaluated in murine models. Results showed that oral immunization with the HIV-PsV DNA vaccine in monkey generated p24 and gp41 epitopes-specific serum IgG. Importantly, these induced antibodies had been shown to neutralize HIV-1 primary strain. In addition, the advantage of VLPs as vehicles delivering genes had been first revealed in biodistribution results. Therefore, orally administered HIV-PsV DNA vaccine was well-tolerated, enhanced the mucosa targeting property of the plasmid DNA, and reduced the nontargeting distribution, which indicate that it would reduce stress associated with systemic vaccination.

  13. File list: His.PSC.05.AllAg.iPS_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.PSC.05.AllAg.iPS_cells hg19 Histone Pluripotent stem cell iPS cells SRX317576,S...077,SRX317607 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/His.PSC.05.AllAg.iPS_cells.bed ...

  14. File list: His.PSC.50.AllAg.iPS_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.PSC.50.AllAg.iPS_cells mm9 Histone Pluripotent stem cell iPS cells SRX977417,SR...RX127376,SRX146530,SRX146522,SRX146547,SRX333561,SRX035985,SRX1090869 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/His.PSC.50.AllAg.iPS_cells.bed ...

  15. File list: ALL.PSC.50.AllAg.iPS_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.PSC.50.AllAg.iPS_cells hg19 All antigens Pluripotent stem cell iPS cells SRX088...16,SRX189400,SRX189399 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/ALL.PSC.50.AllAg.iPS_cells.bed ...

  16. File list: His.PSC.10.AllAg.iPS_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.PSC.10.AllAg.iPS_cells hg19 Histone Pluripotent stem cell iPS cells SRX110016,S...315,SRX381309 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/His.PSC.10.AllAg.iPS_cells.bed ...

  17. File list: ALL.PSC.20.AllAg.iPS_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.PSC.20.AllAg.iPS_cells hg19 All antigens Pluripotent stem cell iPS cells SRX088...27,SRX189400,SRX189399 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/ALL.PSC.20.AllAg.iPS_cells.bed ...

  18. File list: His.PSC.50.AllAg.iPS_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.PSC.50.AllAg.iPS_cells hg19 Histone Pluripotent stem cell iPS cells SRX110015,S...079,SRX317585 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/His.PSC.50.AllAg.iPS_cells.bed ...

  19. File list: Pol.PSC.20.AllAg.iPS_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.20.AllAg.iPS_cells mm9 RNA polymerase Pluripotent stem cell iPS cells SRX97...7435,SRX977434,SRX027462 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.PSC.20.AllAg.iPS_cells.bed ...

  20. File list: ALL.PSC.05.AllAg.iPS_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.PSC.05.AllAg.iPS_cells hg19 All antigens Pluripotent stem cell iPS cells SRX753...00,SRX189399,SRX317607 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/ALL.PSC.05.AllAg.iPS_cells.bed ...

  1. File list: Pol.PSC.10.AllAg.iPS_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.10.AllAg.iPS_cells mm9 RNA polymerase Pluripotent stem cell iPS cells SRX97...7435,SRX977434,SRX027462 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.PSC.10.AllAg.iPS_cells.bed ...

  2. File list: ALL.PSC.05.AllAg.iPS_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.PSC.05.AllAg.iPS_cells mm9 All antigens Pluripotent stem cell iPS cells SRX9774...30,SRX146524,SRX146522,SRX146547 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/ALL.PSC.05.AllAg.iPS_cells.bed ...

  3. File list: Oth.PSC.50.AllAg.iPS_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.PSC.50.AllAg.iPS_cells mm9 TFs and others Pluripotent stem cell iPS cells SRX97...RX146524 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.PSC.50.AllAg.iPS_cells.bed ...

  4. File list: Pol.PSC.50.AllAg.iPS_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.50.AllAg.iPS_cells mm9 RNA polymerase Pluripotent stem cell iPS cells SRX97...7435,SRX977434,SRX027462 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.PSC.50.AllAg.iPS_cells.bed ...

  5. File list: DNS.PSC.20.AllAg.iPS_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.PSC.20.AllAg.iPS_cells hg19 DNase-seq Pluripotent stem cell iPS cells SRX040379...,SRX040378,SRX135563,SRX040377,SRX040376,SRX189427,SRX189400,SRX189399 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/DNS.PSC.20.AllAg.iPS_cells.bed ...

  6. File list: DNS.PSC.10.AllAg.iPS_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.PSC.10.AllAg.iPS_cells hg19 DNase-seq Pluripotent stem cell iPS cells SRX040379...,SRX040378,SRX040377,SRX040376,SRX135563,SRX189427,SRX189400,SRX189399 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/DNS.PSC.10.AllAg.iPS_cells.bed ...

  7. File list: Pol.PSC.05.AllAg.iPS_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.05.AllAg.iPS_cells mm9 RNA polymerase Pluripotent stem cell iPS cells SRX97...7435,SRX027462,SRX977434 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.PSC.05.AllAg.iPS_cells.bed ...

  8. File list: ALL.PSC.10.AllAg.iPS_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.PSC.10.AllAg.iPS_cells hg19 All antigens Pluripotent stem cell iPS cells SRX753...09,SRX189400,SRX189399 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/ALL.PSC.10.AllAg.iPS_cells.bed ...

  9. File list: His.PSC.10.AllAg.iPS_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.PSC.10.AllAg.iPS_cells mm9 Histone Pluripotent stem cell iPS cells SRX977417,SR...RX127372,SRX1090869,SRX127376,SRX035977,SRX146530,SRX146547,SRX146522 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/His.PSC.10.AllAg.iPS_cells.bed ...

  10. File list: Oth.PSC.20.AllAg.iPS_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.PSC.20.AllAg.iPS_cells mm9 TFs and others Pluripotent stem cell iPS cells SRX97...RX146524 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.PSC.20.AllAg.iPS_cells.bed ...

  11. File list: DNS.PSC.05.AllAg.iPS_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.PSC.05.AllAg.iPS_cells hg19 DNase-seq Pluripotent stem cell iPS cells SRX040379...,SRX040378,SRX040377,SRX040376,SRX135563,SRX189427,SRX189400,SRX189399 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/DNS.PSC.05.AllAg.iPS_cells.bed ...

  12. File list: His.PSC.20.AllAg.iPS_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.PSC.20.AllAg.iPS_cells mm9 Histone Pluripotent stem cell iPS cells SRX127389,SR...RX127372,SRX127373,SRX1090869,SRX127376,SRX146530,SRX146522,SRX146547 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/His.PSC.20.AllAg.iPS_cells.bed ...

  13. Genomic imprinting is variably lost during reprogramming of mouse iPS cells.

    Science.gov (United States)

    Takikawa, Sachiko; Ray, Chelsea; Wang, Xin; Shamis, Yulia; Wu, Tien-Yuan; Li, Xiajun

    2013-09-01

    Derivation of induced pluripotent stem (iPS) cells is mainly an epigenetic reprogramming process. It is still quite controversial how genomic imprinting is reprogrammed in iPS cells. Thus, we derived multiple iPS clones from genetically identical mouse somatic cells. We found that parentally inherited imprint was variably lost among these iPS clones. Concurrent with the loss of DNA methylation imprint at the corresponding Snrpn and Peg3 imprinted regions, parental origin-specific expression of the Snrpn and Zim1 imprinted genes was also lost in these iPS clones. This loss of parental genomic imprinting in iPS cells was likely caused by the reprogramming process during iPS cell derivation because extended culture of iPS cells did not lead to significant increase in the loss of genomic imprinting. Intriguingly, one to several paternal chromosomes appeared to have acquired de novo methylation at the Snrpn and Zac1 imprinted regions in a high percentage of iPS clones. These results might have some implications for future therapeutic applications of iPS cells. Since DNA methylation imprint can be completely erased in some iPS clones at multiple imprinted regions, iPS cell reprogramming may also be employed to dissect the underlying mechanisms of erasure, reacquisition and maintenance of genomic imprinting in mammals. Copyright © 2013. Published by Elsevier B.V.

  14. File list: Oth.PSC.10.AllAg.iPS_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.PSC.10.AllAg.iPS_cells mm9 TFs and others Pluripotent stem cell iPS cells SRX65...RX146524 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.PSC.10.AllAg.iPS_cells.bed ...

  15. File list: His.PSC.05.AllAg.iPS_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.PSC.05.AllAg.iPS_cells mm9 Histone Pluripotent stem cell iPS cells SRX977417,SR...RX127374,SRX127373,SRX1090869,SRX333561,SRX146530,SRX146522,SRX146547 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/His.PSC.05.AllAg.iPS_cells.bed ...

  16. File list: ALL.PSC.10.AllAg.iPS_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.PSC.10.AllAg.iPS_cells mm9 All antigens Pluripotent stem cell iPS cells SRX9774...30,SRX146524,SRX146547,SRX146522 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/ALL.PSC.10.AllAg.iPS_cells.bed ...

  17. File list: ALL.PSC.50.AllAg.iPS_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.PSC.50.AllAg.iPS_cells mm9 All antigens Pluripotent stem cell iPS cells SRX9773...1,SRX035985,SRX1090869 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/ALL.PSC.50.AllAg.iPS_cells.bed ...

  18. File list: ALL.PSC.20.AllAg.iPS_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.PSC.20.AllAg.iPS_cells mm9 All antigens Pluripotent stem cell iPS cells SRX9773...30,SRX146522,SRX146547 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/ALL.PSC.20.AllAg.iPS_cells.bed ...

  19. File list: His.PSC.20.AllAg.iPS_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.PSC.20.AllAg.iPS_cells hg19 Histone Pluripotent stem cell iPS cells SRX110015,S...315,SRX381309 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/His.PSC.20.AllAg.iPS_cells.bed ...

  20. File list: Oth.PSC.05.AllAg.iPS_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.PSC.05.AllAg.iPS_cells mm9 TFs and others Pluripotent stem cell iPS cells SRX65...RX146524 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.PSC.05.AllAg.iPS_cells.bed ...

  1. File list: DNS.PSC.50.AllAg.iPS_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.PSC.50.AllAg.iPS_cells hg19 DNase-seq Pluripotent stem cell iPS cells SRX040379...,SRX040378,SRX135563,SRX040376,SRX040377,SRX189427,SRX189400,SRX189399 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/DNS.PSC.50.AllAg.iPS_cells.bed ...

  2. Fruit and seed heteromorphism in the cold desert annual ephemeral Diptychocarpus strictus (Brassicaceae) and possible adaptive significance

    Science.gov (United States)

    Lu, Juanjuan; Tan, Dunyan; Baskin, Jerry M.; Baskin, Carol C.

    2010-01-01

    Background and Aims Diptychocarpus strictus is an annual ephemeral in the cold desert of northwest China that produces heteromorphic fruits and seeds. The primary aims of this study were to characterize the morphology and anatomy of fruits and seeds of this species and compare the role of fruit and seed hetermorphism in dispersal and germination. Methods Shape, size, mass and dispersal of siliques and seeds and the thickness of the mucilage layer on seeds were measured, and the anatomy of siliques and seeds, the role of seed mucilage in water absorption/dehydration, germination and adherence of seeds to soil particles, the role of pericarp of lower siliques in seed dormancy and seed after-ripening and germination phenology were studied using standard procedures. Key Results Plants produce dehiscent upper siliques with a thin pericarp containing seeds with large wings and a thick mucilage layer and indehiscent lower siliques with a thick pericarp containing nearly wingless seeds with a thin mucilage layer. The dispersal ability of seeds from the upper siliques was much greater than that of intact lower siliques. Mucilage increased the amount of water absorbed by seeds and decreased the rate of dehydration. Seeds with a thick mucilage layer adhered to soil particles much better than those with a thin mucilage layer or those from which mucilage had been removed. Fresh seeds were physiologically dormant and after-ripened during summer. Non-dormant seeds germinated to high percentages in light and in darkness. Germination of seeds from upper siliques is delayed until spring primarily by drought in summer and autumn, whereas the thick, indehiscent pericarp prevents germination for >1 year of seeds retained in lower siliques. Conclusions The life cycle of D. strictus is morphologically and physiologically adapted to the cold desert environment in time and space via a combination of characters associated with fruit and seed heteromorphism. PMID:20348559

  3. Ser-261 phospho-regulation is involved in pS256 and pS269-mediated aquaporin-2 apical translocation.

    Science.gov (United States)

    Yui, Naofumi; Ando, Fumiaki; Sasaki, Sei; Uchida, Shinichi

    2017-08-26

    Vasopressin catalyzes aquaporin-2 phosphorylation at several serine sites in the C-terminal region. Compared with Ser-256 and Ser-269 phosphorylation, the role of Ser-261 phospho-regulation on vasopressin-regulated AQP2 apical translocation is largely unknown. In addition, recent discovery of transcytotic apical delivery of AQP2 made the concept of its intracellular trafficking even more complicated. In this study, we evaluated how intact phospho-AQP2 signals fit with the transcytosis trafficking model in Madin-Darby canine kidney cells. PS256 and pS269 signals were intracellularly detectable in wild-type AQP2 at the beginning of forskolin stimulation (1 min). These phospho-signals were detectable in basolateral membranes even after 10 min of stimulation. AQP2 stably inserted in the apical membrane increased pS269 and decreased pS261 signals. In an NDI-causing mutant P262L-AQP2, in which Ser-261 phospho-regulation is impaired, the pS256 and pS269 signals were detectable in the basolateral membranes with increased pS261 signals after forskolin stimulation. These results suggest that Ser-261 phospho-regulation is involved in pS256- and pS269-mediated AQP2 apical translocation. Copyright © 2017 Elsevier Inc. All rights reserved.

  4. Seed thioredoxin h

    DEFF Research Database (Denmark)

    Hägglund, Per; Finnie, Christine; Yano, Hiroyuki

    2016-01-01

    , for example chloroplastic f- and m-type thioredoxins involved in regulation of the Calvin-Benson cycle. The cytosolic h-type thioredoxins act as key regulators of seed germination and are recycled by NADPH-dependent thioredoxin reductase. The present review on thioredoxin h systems in plant seeds focuses...

  5. Seed-mediated growth of patterned graphene nanoribbon arrays

    Energy Technology Data Exchange (ETDEWEB)

    Arnold, Michael Scott; Way, Austin James; Jacobberger, Robert Michael

    2017-09-12

    Graphene nanoribbon arrays, methods of growing graphene nanoribbon arrays, and electronic and photonic devices incorporating the graphene nanoribbon arrays are provided. The graphene nanoribbons in the arrays are formed using a seed-mediated, bottom-up, chemical vapor deposition (CVD) technique in which the (001) facet of a semiconductor substrate and the orientation of the seed particles on the substrate are used to orient the graphene nanoribbon crystals preferentially along a single [110] direction of the substrate.

  6. The particle suppliers

    CERN Multimedia

    CERN Bulletin

    2010-01-01

    Particles are supplied to the LHC by six accelerators inter-connected by several kilometres of transfer lines. This represents yet another complex chain of processes whereby particles are produced, bunched, synchronised and injected into the LHC at the precise moment it's ready to receive them. In other words, for collisions to be produced at the end of the chain, all the injectors must be in perfect working order.   Among all the questions asked by the many visitors to CERN, one in particular comes up time and time again: "Why don't you just connect the LHC directly to the proton source?" In other words, why do you need this whole chain of accelerators acting as an "injector" for the LHC? Before colliding inside the LHC, particles first have to pass through no fewer than six different accelerators: the 90 keV duoplasmatron source, the 750 keV RFQ, the 50 MeV Linac 2, the 1.4 GeV synchrotron injector ("PS Booster" or PSB), the 25 GeV Proton Sy...

  7. MERIT - The high intensity liquid mercury target experiment at the CERN PS

    CERN Document Server

    Efthymiopoulos, I

    2009-01-01

    The MERIT experiment is a proof-of-principle test of a target system for high power proton beams to be used as front-end for a Neutrino Factory complex or a Muon Collider. The experiment took data in autumn 2007 with the fast extracted beam from the CERN Proton Synchrotron (PS) to a maximum intensity of about 30 × 1012 protons per pulse. The target system, based on a free mercury jet, allowed investigation of the interseption of a 4-MW proton beam inside a 15-T magnetic field required to capture the low-energy secondary pions as the source of the required intense muon beams. Particle detectors have been installed around the target setup to measure the secondary particle flux out of the target and probe cavitation effects in the mercury jet when exited with a beam of variable intensity. With the analysis of the data ongoing, results will be presented here that demonstrate the validity of the liquid target concept.

  8. A rule-based consultant for accelerator beam scheduling used in the CERN PS complex

    CERN Document Server

    Lewis, J; Varga, L Z

    1995-01-01

    The CERN PS accelerator complex consists of nine interacting accelerators which work together to produce particle beams for different end users, varying in particle type, energy, time structure, and geometry. The beam production schedule is time sliced, and depends on the current operational requirements, and dynamically on the accelerator status, so that production schedule changes occur in real time. Many potential schedules are not valid due to various system constraints and these constraints vary over time as new operational modes are introduced. In order to ensure that only valid schedules are given to the complex, an automated tool has been developed to indicate whether a potential schedule is valid or not. This presentation describes the method by which the validity of a beam schedule is determined and how this method was implemented using a rule-based approach based on SQL, avoiding the use of an expert system shell. Both the data to instantiate the rules and the rules themselves are kept in an Oracle...

  9. Create and Publish a Hierarchical Progressive Survey (HiPS)

    Science.gov (United States)

    Fernique, P.; Boch, T.; Pineau, F.; Oberto, A.

    2014-05-01

    Since 2009, the CDS promotes a method for visualizing based on the HEALPix sky tessellation. This method, called “Hierarchical Progressive Survey" or HiPS, allows one to display a survey progressively. It is particularly suited for all-sky surveys or deep fields. This visualization method is now integrated in several applications, notably Aladin, the SiTools/MIZAR CNES framework, and the recent HTML5 “Aladin Lite". Also, more than one hundred surveys are already available in this view mode. In this article, we will present the progress concerning this method and its recent adaptation to the astronomical catalogs such as the GAIA simulation.

  10. Electrostatic septum for "Continuous Transfer" from PS to SPS

    CERN Multimedia

    CERN PhotoLab

    1982-01-01

    For "Continuous Transfer" to the SPS, the PS beam, after acceleration, is peeled off in 5 turns. To minimize losses, the magnetic septa are preceded by an electrostatic septum in straight section 31. We see the inner part of it, on a lab-bench. The first part consists of W-wires, the second part is a Mo-foil. The circulating beam passes through the opening, the ejected beam at the outside (above the wires, in this picture). This assembly is the anode-part, the cathode is not shown.

  11. Beam Quality Preservation in the CERN PS-SPS Complex

    CERN Multimedia

    Arduini, Gianluigi

    2004-01-01

    The LHC will require beams of unprecedented transverse and longitudinal brightness. Their production imposes tight constraints on the emittance growth in each element of the LHC injector chain, namely the PS-SPS Accelerator Complex. The problems encountered at the different stages of the acceleration in the complex span a wide range of topics, such as injection matching, RF gymnastics, space charge, transverse and longitudinal single- and coupled-bunch instabilities, and electron cloud effects. The measurement techniques developed and applied to identify and study the various sources of emittance dilution to the high precision required for the LHC beams and the solutions found to control such phenomena are illustrated.

  12. Lo irreductible social y lo irreductible psíquico

    Directory of Open Access Journals (Sweden)

    Vincent Gaulejac, de

    2017-06-01

    Full Text Available Con base en la reconstrucción de las polaridades explicativas -lo irreductible social y lo irreductible psíquico-que atraviesan a las ciencias sociales, este texto propone trascender los modelos antagónicos y excluyentes. El objetivo es instaurar en el centro de la reflexión la idea de la dialktica existencial que restituye al sujeto tanto el contexto socio-histórico en el cual está localizado como el deseo y la singularidad que lo constituyen en productor de la afirmación de su individualidad y su historicidad.

  13. PS potential performance with a higher injection energy

    CERN Document Server

    Gilardoni, S; Borburgh, J; Bodart, D; Chiggiato, P; Damerau, H; Hancock, S; Metral, G; Pittet, S; Rossi, C; Rumolo, G; Steerenberg, R; Widorski, M

    2011-01-01

    In the context of the LHC Injectors upgrade project, the PS has to be brought up to − and to operate reliably at − the level of performance required by the HL-LHC until the end of the LHC lifetime. The study has started on the potential benefits of increasing the injection energy. An overview of the impact of this upgrade will be presented, with a preliminary estimate of the beam characteristics at the SPS entrance and the remaining performance limitations. The necessary hardware modifications will be described, highlighting the critical systems and the risks. The program for the 2011 machine studies and hardware interventions for refining these plans will be presented.

  14. Injection and transfer lines of the PS Booster

    CERN Multimedia

    Photographic Service

    1972-01-01

    In the foreground is the vacuum chamber for the 50 MeV proton beam coming from the Linac. The tank held by white frames houses the "Vertical Distributor", which deflects the Linac beam to the levels of the Booster's 4 superposed rings. After acceleration in the Booster, originally to 800 MeV, today to 1.4 GeV, the beams from the 4 rings are combined in the vertical plane and transfered to the 26 GeV PS. The "Recombination Line", intersecting the injection line, crosses the picture from left to right.

  15. Search for Decays of Heavy Neutrinos with the PS Beam

    CERN Multimedia

    2002-01-01

    The experiment searches for neutrino decay, primarily into the e|+e|-@n^e and @g@g@n^e modes. Neutrino masses in the region between 1 and 400~MeV will be explored. The beam used is the neutrino PS beam used for the oscillation experiments. The apparatus consists of a decay volume @=30~m long and a calorimeter @=8~radiation lengths thick and @=20~m|2 in surface. The detectors are flash-tube modules of the type developed at Saclay for the proton-stability experiment. Scintillator hodoscopes give the timing information necessary for the trigger logic and background rejection.

  16. Reliability and maintenance analysis of the CERN PS booster

    CERN Document Server

    Staff, P S B

    1977-01-01

    The PS Booster Synchrotron being a complex accelerator with four superposed rings and substantial additional equipment for beam splitting and recombination, doubts were expressed at the time of project authorization as to its likely operational reliability. For 1975 and 1976, the average down time was 3.2% (at least one ring off) or 1.5% (all four rings off). The items analysed are: operational record, design features, maintenance, spare parts policy, operating temperature, effects of thunderstorms, fault diagnostics, role of operations staff and action by experts. (15 refs).

  17. Viscometric characterization of PS/POSS hybrid nanocomposites

    OpenAIRE

    Bianchi, Otávio; Repenning, Gustavo B.; Mauler, Raquel S.; Oliveira, Ricardo V. B.; Canto, Leonardo B.

    2012-01-01

    Nanocompósitos híbridos de poliestireno (PS) e poliedros oligoméricos silsesquioxanos (POSS) com diferentes composições e graus de hibridização foram obtidos por processamento reativo no estado fundido utilizando-se peróxido de dicumila (DCP) como iniciador, na presença ou não de estireno como agente de transferência de radical. Os materiais foram caracterizados viscosimetricamente por cromatografia de permeação em gel (GPC) usando detecção tripla por espalhamento de luz, viscosimetria e índi...

  18. A multiturn measurement system for the CERN PS

    CERN Document Server

    Angoletta, Maria Elena

    2002-01-01

    Multiturn beam position measurements on one or more pickups provide very important information needed to derive machine optics parameters. A variety of analyses is possible, such as determination of phase advance, detuning with amplitude, and most important, the exploration of phase space. In this paper we present a new multiturn acquisition system for the CERN proton synchrotron (CERN PS) based on a compact PCI fast digitiser and a new general object-oriented visualisation and analysis tool for the acquired multiturn data. (11 refs).

  19. Tissue-specific regulation of gibberellin biosynthesis in developing pea seeds.

    Science.gov (United States)

    Nadeau, Courtney D; Ozga, Jocelyn A; Kurepin, Leonid V; Jin, Alena; Pharis, Richard P; Reinecke, Dennis M

    2011-06-01

    Previous work suggests that gibberellins (GAs) play an important role in early seed development. To more fully understand the roles of GAs throughout seed development, tissue-specific transcription profiles of GA metabolism genes and quantitative profiles of key GAs were determined in pea (Pisum sativum) seeds during the seed-filling development period (8-20 d after anthesis [DAA]). These profiles were correlated with seed photoassimilate acquisition and storage as well as morphological development. Seed coat growth (8-12 DAA) and the subsequent dramatic expansion of branched parenchyma cells were correlated with both transcript abundance of GA biosynthesis genes and the concentration of the growth effector GA, GA(1). These results suggest GA(1) involvement in determining the rate of seed coat growth and sink strength. The endosperm's PsGA20ox transcript abundance and the concentration of GA(20) increased markedly as the endosperm reached its maximum volume (12 DAA), thus providing ample GA(20) substrate for the GA 3-oxidases present in both the embryo and seed coat. Furthermore, PsGA3ox transcript profiles and trends in GA(1) levels in embryos at 10 to 16 DAA and also in embryo axes at 18 DAA suggest localized GA(1)-induced growth in these tissues. A shift from synthesis of GA(1) to that of GA(8) occurred after 18 DAA in the embryo axis, suggesting that deactivation of GA(1) to GA(8) is a likely mechanism to limit embryo axis growth and allow embryo maturation to proceed. We hypothesize that GA biosynthesis and catabolism are tightly regulated to bring about the unique developmental events that occur during seed growth, development, and maturation.

  20. Tissue-Specific Regulation of Gibberellin Biosynthesis in Developing Pea Seeds1[W][OA

    Science.gov (United States)

    Nadeau, Courtney D.; Ozga, Jocelyn A.; Kurepin, Leonid V.; Jin, Alena; Pharis, Richard P.; Reinecke, Dennis M.

    2011-01-01

    Previous work suggests that gibberellins (GAs) play an important role in early seed development. To more fully understand the roles of GAs throughout seed development, tissue-specific transcription profiles of GA metabolism genes and quantitative profiles of key GAs were determined in pea (Pisum sativum) seeds during the seed-filling development period (8–20 d after anthesis [DAA]). These profiles were correlated with seed photoassimilate acquisition and storage as well as morphological development. Seed coat growth (8–12 DAA) and the subsequent dramatic expansion of branched parenchyma cells were correlated with both transcript abundance of GA biosynthesis genes and the concentration of the growth effector GA, GA1. These results suggest GA1 involvement in determining the rate of seed coat growth and sink strength. The endosperm’s PsGA20ox transcript abundance and the concentration of GA20 increased markedly as the endosperm reached its maximum volume (12 DAA), thus providing ample GA20 substrate for the GA 3-oxidases present in both the embryo and seed coat. Furthermore, PsGA3ox transcript profiles and trends in GA1 levels in embryos at 10 to 16 DAA and also in embryo axes at 18 DAA suggest localized GA1-induced growth in these tissues. A shift from synthesis of GA1 to that of GA8 occurred after 18 DAA in the embryo axis, suggesting that deactivation of GA1 to GA8 is a likely mechanism to limit embryo axis growth and allow embryo maturation to proceed. We hypothesize that GA biosynthesis and catabolism are tightly regulated to bring about the unique developmental events that occur during seed growth, development, and maturation. PMID:21482633

  1. Genetics and Forest Seed Handling

    DEFF Research Database (Denmark)

    Schmidt, Lars Holger

    2016-01-01

    High genetic quality seed is obtained from seed sources that match the planting site, have a good outcrossing rate, and are superior in some desirable characters. Non-degraded natural forests and plantations may be used as untested seed sources, which can sometimes be managed to promote outbreeding...... and increase seed production. Planted seed orchards aim at capturing large genetic variation and are planted in a design that facilitates genetic evaluation and promotes outbred seed production. Good seed production relies upon success of the whole range of reproductive events from flower differentiation...... to seed maturation. Physiologically sound seed shows a high germination rate, but germination may be restricted by various dormancy mechanisms that in nature aim at delaying or restricting seed germination during time of poor seed survival....

  2. Effects of Pig Slurry as Basal and Panicle Fertilizer on Trace Element Content and Grain Quality in Direct-Seeding Rice

    Directory of Open Access Journals (Sweden)

    Lifen Huang

    2016-07-01

    Full Text Available Direct-seeding rice has grown in popularity in recent years and is widespread in China. This study focused on the effects of pig slurry (PS on grain quality and safety in direct-seeding rice. Application of more than 210 m3·hm−2 increased rice yield and dry matter accumulation, compared with conventional chemical fertilizer treatment (CK2. At the heading and maturing stage, a high dosage of PS (T6, T8 and T9 treatments promoted uptake of Pb, Cu, Zn, Fe and Mn by rice plants and grain, but retarded uptake of Cr, Co and Ni, indicating an ability to alleviate toxicity of some heavy metals. Compared with CK2, PS alleviated the transfer of Pb, Cr, Co, Ni and Cu from straw to grain, but enhanced the transport of Zn, Fe and Mn. Although PS application slightly diminished the processing quality, including brown rice, milled rice and head milled rice, it significantly decreased the amylose content, optimized the starch profile, and improved the overall quality. The results confirmed the feasibility and safety of PS application to direct-seeding rice, and determined that PS basal and panicle fertilizer could increase yield and improve the rice quality when applied between 210 and 240 m3·hm−2.

  3. TRPV-1-mediated elimination of residual iPS cells in bioengineered cardiac cell sheet tissues

    Science.gov (United States)

    Matsuura, Katsuhisa; Seta, Hiroyoshi; Haraguchi, Yuji; Alsayegh, Khaled; Sekine, Hidekazu; Shimizu, Tatsuya; Hagiwara, Nobuhisa; Yamazaki, Kenji; Okano, Teruo

    2016-01-01

    The development of a suitable strategy for eliminating remaining undifferentiated cells is indispensable for the use of human-induced pluripotent stem (iPS) cell-derived cells in regenerative medicine. Here, we show for the first time that TRPV-1 activation through transient culture at 42 °C in combination with agonists is a simple and useful strategy to eliminate iPS cells from bioengineered cardiac cell sheet tissues. When human iPS cells were cultured at 42 °C, almost all cells disappeared by 48 hours through apoptosis. However, iPS cell-derived cardiomyocytes and fibroblasts maintained transcriptional and protein expression levels, and cardiac cell sheets were fabricated after reducing the temperature. TRPV-1 expression in iPS cells was upregulated at 42 °C, and iPS cell death at 42 °C was TRPV-1-dependent. Furthermore, TRPV-1 activation through thermal or agonist treatment eliminated iPS cells in cardiac tissues for a final concentration of 0.4% iPS cell contamination. These findings suggest that the difference in tolerance to TRPV-1 activation between iPS cells and iPS cell-derived cardiac cells could be exploited to eliminate remaining iPS cells in bioengineered cell sheet tissues, which will further reduce the risk of tumour formation. PMID:26888607

  4. TRPV-1-mediated elimination of residual iPS cells in bioengineered cardiac cell sheet tissues.

    Science.gov (United States)

    Matsuura, Katsuhisa; Seta, Hiroyoshi; Haraguchi, Yuji; Alsayegh, Khaled; Sekine, Hidekazu; Shimizu, Tatsuya; Hagiwara, Nobuhisa; Yamazaki, Kenji; Okano, Teruo

    2016-02-18

    The development of a suitable strategy for eliminating remaining undifferentiated cells is indispensable for the use of human-induced pluripotent stem (iPS) cell-derived cells in regenerative medicine. Here, we show for the first time that TRPV-1 activation through transient culture at 42 °C in combination with agonists is a simple and useful strategy to eliminate iPS cells from bioengineered cardiac cell sheet tissues. When human iPS cells were cultured at 42 °C, almost all cells disappeared by 48 hours through apoptosis. However, iPS cell-derived cardiomyocytes and fibroblasts maintained transcriptional and protein expression levels, and cardiac cell sheets were fabricated after reducing the temperature. TRPV-1 expression in iPS cells was upregulated at 42 °C, and iPS cell death at 42 °C was TRPV-1-dependent. Furthermore, TRPV-1 activation through thermal or agonist treatment eliminated iPS cells in cardiac tissues for a final concentration of 0.4% iPS cell contamination. These findings suggest that the difference in tolerance to TRPV-1 activation between iPS cells and iPS cell-derived cardiac cells could be exploited to eliminate remaining iPS cells in bioengineered cell sheet tissues, which will further reduce the risk of tumour formation.

  5. [Induced pluripotent stem (iPS) cell - issues for clinical application - ].

    Science.gov (United States)

    Aoi, Takashi

    2013-01-01

    Induced pluripotent stem (iPS) cells are generated from somatic cells by introducing small sets of transcription factors. iPS cells demonstrate pluripotency and the ability to self-renew. In addition, iPS cells can be generated from donor individuals with particular characteristics. Based on these features, iPS cells are expected to be applicable in drug discovery, the study of disease mechanisms and cell therapy. From a technical point of view, "diversity" is the key word. At present, iPS cells can be derived using various techniques, resulting in diversity in the quality of iPS cells generated. Therefore, optimization of the derivation technology is one of the most important issues. Another "diversity" is in the propensities amongst iPS cell lines derived using similar techniques. Thus, strategies for selecting good quality lines remain to be established. Considering such technical hurdles, establishment of an iPS cell bank consisting of high quality and versatile iPS lines is a promising idea because of the merits of cost and quality control. Now, we are exploring relevant parameters for the quality control of banked cells. The challenges facing clinical application of iPS cells are new but not unprecedented. To realize clinical applications of iPS cells, we need to make these challenges clear and overcome them through partnership not only with industry, governments and universities, but also patients and society at large.

  6. Glioblastoma with spinal seeding

    Energy Technology Data Exchange (ETDEWEB)

    Fakhrai, N.; Fazeny-Doerner, B.; Marosi, C. [Clinical Div. of Oncology, Dept. of Medicine I, Univ. of Vienna (Austria); Czech, T. [Dept. of Neurosurgery, Univ. of Vienna (Austria); Diekmann, K. [Dept. of Radiooncology, Univ. of Vienna (Austria); Birner, P.; Hainfellner, J.A. [Clinical Inst. for Neurology, Univ. of Vienna (Austria); Prayer, D. [Dept. of Neuroradiology, Univ. of Vienna (Austria)

    2004-07-01

    Background: extracranial seeding of glioblastoma multiforme (GBM) is very rare and its development depends on several factors. This case report describes two patients suffering from GBM with spinal seeding. In both cases, the anatomic localization of the primary tumor close to the cerebrospinal fluid (CSF) was the main factor for spinal seeding. Case reports: two patients with GBM and spinal seeding are presented. After diagnosis of spinal seeding, both patients were highly symptomatic from their spinal lesions. Case 1 experienced severe pain requiring opiates, and case 2 had paresis of lower limbs as well as urinary retention/incontinence. Both patients were treated with spinal radiation therapy. Nevertheless, they died 3 months after diagnosis of spinal seeding. Results: in both patients the diagnosis of spinal seeding was made at the time of cranial recurrence. Both tumors showed close contact to the CSF initially. Even though the patients underwent intensive treatment, it was not possible to keep them in a symptom-free state. Conclusion: because of short survival periods, patients deserve optimal pain management and dedicated palliative care. (orig.)

  7. Effect of Genotypes and Seed Production Environments on Seed ...

    African Journals Online (AJOL)

    Seeds from each harvest were subjected to different seed quality tests and data generated were analyzed. Seed quality traits were considerably affected by the genotypes and growing conditions i.e. plant population and cropping seasons. Genotypes with superior seed quality were prevalent at 166.667 and 266.667 plants ...

  8. Effects of seed fermentation method on seed germination and vigor ...

    African Journals Online (AJOL)

    The present study was conducted to examine the influence of Lagenaria siceraria seed fermentation method on seed germination and vigor. Three seed fermentation methods (fermented in ambient air, plastic bag stored in ambient or in plastic bag buried) were tested on two cultivars during two years. Seed germination and ...

  9. Methods for iPS cell generation for basic research and clinical applications.

    Science.gov (United States)

    Mochiduki, Yuji; Okita, Keisuke

    2012-06-01

    The induction of pluripotency can be achieved by forced expression of defined factors in somatic cells. The established cells, termed induced pluripotent stem (iPS) cells, have pluripotency and an infinite capacity for self-renewal in common with embryonic stem (ES) cells. Patient-specific iPS cells could be a useful source for drug discovery and cell transplantation therapies; however, the original method for iPS cell generation had several issues that were obstacles to their clinical application. Recent studies have brought about various improvements for iPS cell generation and uncovered several characteristics of iPS cells. Here we summarize the current status of iPS cell studies, with a focus on the improved methods that can be used to generate iPS cells, and also refer to the future challenges. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  10. Protein-based polymers that bond to DNA : design of virus-like particles and supramolecular nanostructures

    NARCIS (Netherlands)

    Hernandez Garcia, A.

    2014-01-01

     In this thesis it is demonstrated that it is possible to use Protein-based Polymers (PbPs) as synthetic binders of DNA (or any other negatively charged polyelectrolyte). The PbPs co-assemble with their DNA templates to form highly organized virus-like particles and supramolecular structures. A

  11. Properties of Extruded PS-212 Type Self-Lubricating Materials

    Science.gov (United States)

    Waters, W. J.; Sliney, H. E.; Soltis, R. F.

    1993-01-01

    Research has been underway at the NASA Lewis Research Center since the 1960's to develop high temperature, self-lubricating materials. The bulk of the research has been done in-house by a team of researchers from the Materials Division. A series of self-lubricating solid material systems has been developed over the years. One of the most promising is the composite material system referred to as PS-212 or PM-212. This material is a powder metallurgy product composed of metal bonded chromium carbide and two solid lubricating materials known to be self-lubricating over a wide temperature range. NASA feels this material has a wide potential in industrial applications. Simplified processing of this material would enhance its commercial potential. Processing changes have the potential to reduce processing costs, but tribological and physical properties must not be adversely affected. Extrusion processing has been employed in this investigation as a consolidation process for PM-212/PS-212. It has been successful in that high density bars of EX-212 (extruded PM-212) can readily be fabricated. Friction and strength data indicate these properties have been maintained or improved over the P.M. version. A range of extrusion temperatures have been investigated and tensile, friction, wear, and microstructural data have been obtained. Results indicate extrusion temperatures are not critical from a densification standpoint, but other properties are temperature dependent.

  12. Phase morphological study on SEBS compatibilized PS/LDPE blends

    Directory of Open Access Journals (Sweden)

    Chatchai Kunyawut

    2014-09-01

    Full Text Available The co-continuous phase morphology of polystyrene (PS/low density polyethylene (LDPE blends compatibilized with poly(styrene-block-ethylene/butylene-block-styrene triblock copolymers (SEBS with varying molecular weights has been investigated. The blend samples were prepared in a mini-twin screw extruder. The barrel length and diameter are 224 and 16 mm, respectively. The diameter of the capillary die is 1 mm. The concentration of the blends was 70/30 wt% of PS/LDPE while that of the SEBS used was 5 wt% of the blend. The mixing temperatures used were 180, 250, and 280o C, and a screw speed of 60 rpm. The morphology of the blends was investigated using an AFM technique. Average droplet diameters of the blend samples were determined using an OM technique. The co-continuous morphology has not been obtained in all the blends, although the mixing temperature used is as high as 280o C. The experimental results indicated that the model prediction of the co-continuous morphology proposed by Willemse and co-worker was not applicable to the blend systems studied. Only droplet-type dispersion was observed. This is considered to arise from the processing conditions and the mixing device used. The blend compatibilized with the high molecular weight SEBS had higher dispersed phase size than that of the blend compatibilized with the medium and low molecular weight SEBSs. This behaviour is likely to arise from coalescence during melt processing.

  13. Preparations for Upgrading the RF Systems of the PS Booster

    CERN Document Server

    Albright, Simon; Shaposhnikova, Elena

    2016-01-01

    The accelerators of the LHC injector chain need to be upgraded to provide the HL-LHC beams. The PS Booster, the first synchrotron in the LHC injection chain, uses three different RF systems (first, second and up to tenth harmonic) in each of its four rings. As part of the LHC Injector Upgrade the current ferrite RF systems will be replaced with broadband Finemet cavities, increasing the flexibility of the RF system. A Finemet test cavity has been installed in Ring 4 to investigate its effect on machine performance, especially beam stability, during extensive experimental studies. Due to large space charge impedance Landau damping is lost through most of the cycle in single harmonic operation, but is recovered when using the second harmonic and controlled longitudinal emittance blow-up. This paper compares beam parameters during acceleration with and without the Finemet test cavity. Comparisons were made using beam measurements and simulations with the BLonD code based on a full PS Booster impedance model. Thi...

  14. KSR-based medium improves the generation of high-quality mouse iPS cells.

    Science.gov (United States)

    Liu, Kai; Wang, Fang; Ye, Xiaoying; Wang, Lingling; Yang, Jiao; Zhang, Jingzhuo; Liu, Lin

    2014-01-01

    Induced pluripotent stem (iPS) cells from somatic cells have great potential for regenerative medicine. The efficiency in generation of iPS cells has been significantly improved in recent years. However, the generation of high-quality iPS cells remains of high interest. Consistently, we demonstrate that knockout serum replacement (KSR)-based medium accelerates iPS cell induction and improves the quality of iPS cells, as confirmed by generation of chimeras and all iPS cell-derived offspring with germline transmission competency. Both alkaline phosphatase (AP) activity assay and expression of Nanog have been used to evaluate the efficiency of iPS cell induction and formation of ES/iPS cell colonies; however, appropriate expression of Nanog frequently indicates the quality of ES/iPS cells. Interestingly, whereas foetal bovine serum (FBS)-based media increase iPS cell colony formation, as revealed by AP activity, KSR-based media increase the frequency of iPS cell colony formation with Nanog expression. Furthermore, inhibition of MAPK/ERK by a specific inhibitor, PD0325901, in KSR- but not in FBS-based media significantly increases Nanog-GFP+ iPS cells. In contrast, addition of bFGF in KSR-based media decreases proportion of Nanog-GFP+ iPS cells. Remarkably, PD can rescue Nanog-GFP+ deficiency caused by bFGF. These data suggest that MAPK/ERK pathway influences high quality mouse iPS cells and that KSR- and PD-based media could enrich homogeneous authentic pluripotent stem cells.

  15. MicroRNA expression profiles of human iPS cells, retinal pigment epithelium derived from iPS, and fetal retinal pigment epithelium.

    Science.gov (United States)

    Greene, Whitney A; Muñiz, Alberto; Plamper, Mark L; Kaini, Ramesh R; Wang, Heuy-Ching

    2014-06-24

    The objective of this report is to describe the protocols for comparing the microRNA (miRNA) profiles of human induced-pluripotent stem (iPS) cells, retinal pigment epithelium (RPE) derived from human iPS cells (iPS-RPE), and fetal RPE. The protocols include collection of RNA for analysis by microarray, and the analysis of microarray data to identify miRNAs that are differentially expressed among three cell types. The methods for culture of iPS cells and fetal RPE are explained. The protocol used for differentiation of RPE from human iPS is also described. The RNA extraction technique we describe was selected to allow maximal recovery of very small RNA for use in a miRNA microarray. Finally, cellular pathway and network analysis of microarray data is explained. These techniques will facilitate the comparison of the miRNA profiles of three different cell types.

  16. Particle detectors

    CERN Multimedia

    CERN. Geneva

    1999-01-01

    Introduction, interaction of radiation with matter measurement of momentum of charged particles, of energy of e/gamma, hadrons, identification of particles. Design of HEP detectors. Principle of operation and performance of tracking sub-detectors, calorimeters and muon system.

  17. What Are Chia Seeds?

    Science.gov (United States)

    ... help improve cardiovascular risk factors such as lowering cholesterol, triglycerides and blood pressure. However, there are not ... ground or whole chia seeds on cereal, rice, yogurt or vegetables. In Mexico, a dish called chia ...

  18. Seed collection notes

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This document contains tables, lists, and notes related to tallgrass prairie seed collection on Neal Smith National Wildlife Refuge in 1992.

  19. Concerning seed spots

    Science.gov (United States)

    J. A. Larsen; R. J. Smith

    1913-01-01

    In connection with the sowing of Yellow pine, White pine and Western larch on The Blackfeet National Forest during the sea sons of 1911 and 1912, seventeen and one-half acres were sowed directly in seed spots.

  20. Prescribed seed plantings

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This document contains memos, notes, and tables related to tallgrass prairie seed harvesting on Neal Smith National Wildlife Refuge in 1995.

  1. Gas Bubbles Stabilized by Janus Particles with Varying Hydrophilic-Hydrophobic Surface Characteristics.

    Science.gov (United States)

    Fujii, Syuji; Yokoyama, Yuichi; Nakayama, Saori; Ito, Masanori; Yusa, Shin-Ichi; Nakamura, Yoshinobu

    2018-01-23

    Micrometer-sized polymer-grafted gold-silica (Au-SiO2) Janus particles were fabricated by vacuum evaporation followed by polymer grafting. The Janus particle diameter, diameter distribution, morphology, surface chemistry, and water wettability were characterized by optical microscopy, scanning electron microscopy, X-ray photoelectron spectroscopy, and contact angle measurements. The optical microscopy results showed that the polystyrene (PS)-grafted Au-SiO2 Janus particles exhibited monolayer adsorption at the air-water interface and could stabilize bubbles, preventing their coalescence for more than 1 month. The hydrophobic PS-grafted Au and hydrophilic SiO2 surfaces were exposed to the air and water phases, respectively. Bare Au-SiO2 and poly(2-(perfluorobutyl)ethyl methacrylate) (PPFBEM)-grafted Au-SiO2 Janus particles could also stabilize bubbles for up to 2 weeks. By contrast, bare silica particles did not stabilize bubbles and were dispersed in water. The bubbles that formed in the PS-grafted Janus particle system were more stable than those formed in the bare Au-SiO2 Janus particles, PPFBEM-grafted Au-SiO2 Janus particles, and SiO2 particle systems because of the high adsorption energy of the PS-grafted particles at the air-water interface.

  2. Comparison of implosion core metrics: A 10 ps dilation X-ray imager vs a 100 ps gated microchannel plate

    Science.gov (United States)

    Nagel, S. R.; Benedetti, L. R.; Bradley, D. K.; Hilsabeck, T. J.; Izumi, N.; Khan, S.; Kyrala, G. A.; Ma, T.; Pak, A.

    2016-11-01

    The dilation x-ray imager (DIXI) [T. J. Hilsabeck et al., Rev. Sci. Instrum. 81, 10E317 (2010); S. R. Nagel et al., ibid. 83, 10E116 (2012); S. R. Nagel et al., ibid. 85, 11E504 (2014)] is a high-speed x-ray framing camera that uses the pulse-dilation technique to achieve a temporal resolution of less than 10 ps. This is a 10 × improvement over conventional framing cameras currently employed on the National Ignition Facility (NIF) (100 ps resolution), and otherwise only achievable with 1D streaked imaging. A side effect of the dramatically reduced gate width is the comparatively lower detected signal level. Therefore we implement a Poisson noise reduction with non-local principal component analysis method [J. Salmon et al., J. Math. Imaging Vision 48, 279294 (2014)] to improve the robustness of the DIXI data analysis. Here we present results on ignition-relevant experiments at the NIF using DIXI. In particular we focus on establishing that/when DIXI gives reliable shape metrics (P0, P2, and P4 Legendre modes, and their temporal evolution/swings).

  3. Determination of Beam Intensity and Position in a Particle Accelerator

    CERN Document Server

    Kasprowicz, Grzegorz

    2010-01-01

    The Proton Synchrotron accelerator (PS), installed at CERN, although commissioned in 1959, still plays a central role in the production of beams for the Antiproton Decelerator, Super Proton Synchrotron, various experimental areas and for the Large Hadron Collider (LHC). The PS produces beams of different types of particles, mainly protons, but also various species of ions. Almost all these particle beams pass through the PS. The quality of the beams delivered to the LHC has a direct impact on the effective luminosity, and therefore the performance of the instrumentation of the PS is of great importance. The old trajec- tory and orbit measurement system of the PS dated back to 1988 and no longer fulfilled present day requirements. It used 40 beam position monitors (BPMs) and an analogue signal processing chain to acquire the trajectory of one single particle bunch out of many, over two consecutive turns at a maximum rate of once every 5ms. The BPMs were in good condition, however the electronics was aging and ...

  4. A role for seed storage proteins in Arabidopsis seed longevity.

    Science.gov (United States)

    Nguyen, Thu-Phuong; Cueff, Gwendal; Hegedus, Dwayne D; Rajjou, Loïc; Bentsink, Leónie

    2015-10-01

    Proteomics approaches have been a useful tool for determining the biological roles and functions of individual proteins and identifying the molecular mechanisms that govern seed germination, vigour and viability in response to ageing. In this work the dry seed proteome of four Arabidopsis thaliana genotypes, that carry introgression fragments at the position of seed longevity quantitative trait loci and as a result display different levels of seed longevity, was investigated. Seeds at two physiological states, after-ripened seeds that had the full germination ability and aged (stored) seeds of which the germination ability was severely reduced, were compared. Aged dry seed proteomes were markedly different from the after-ripened and reflected the seed longevity level of the four genotypes, despite the fact that dry seeds are metabolically quiescent. Results confirmed the role of antioxidant systems, notably vitamin E, and indicated that protection and maintenance of the translation machinery and energy pathways are essential for seed longevity. Moreover, a new role for seed storage proteins (SSPs) was identified in dry seeds during ageing. Cruciferins (CRUs) are the most abundant SSPs in Arabidopsis and seeds of a triple mutant for three CRU isoforms (crua crub cruc) were more sensitive to artificial ageing and their seed proteins were highly oxidized compared with wild-type seeds. These results confirm that oxidation is involved in seed deterioration and that SSPs buffer the seed from oxidative stress, thus protecting important proteins required for seed germination and seedling formation. © The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology.

  5. Storage of sunflower seeds

    Directory of Open Access Journals (Sweden)

    Denise de Castro Lima

    Full Text Available The sunflower is among the top five crops in the world for the production of edible vegetable oil. The species displays rustic behavior, with an excellent edaphic and climatic adaptability index, being able to be cultivated throughout Brazil. Seed quality is the key to increasing production and productivity in the sunflower. The objective of this work was to monitor the viability of sunflower seeds with a view to their conservation when stored in different environments and packaging. The seeds were packed in paper bags, multilayered paper, black polyethylene and PET bottles; and stored for a period of twelve months in the following environments: dry cold room (10 ºC and 55% RH, the ambient conditions of Fortaleza, Ceará, Brazil (30-32 ºC and 75% RH, refrigerator (4 ºC and 38-43% RH and freezer (-20 ºC. Every three months, the water content of the seeds was determined and germination, accelerated ageing, speed of emergence index, and seedling dry weight were evaluated. The experimental design was completely randomized, in a scheme of split-lots, with four replications. It can be concluded that the natural environment is not suitable for the storage of sunflower seeds. Sunflower seeds remain viable for 12 months when stored in a dry cold room, refrigerator or freezer, irrespective of the type of packaging used.

  6. Hydrogen sulfide down-regulates BACE1 and PS1 via activating PI3K/Akt pathway in the brain of APP/PS1 transgenic mouse.

    Science.gov (United States)

    He, Xuan-Li; Yan, Ning; Chen, Xiao-Shan; Qi, Yun-Wen; Yan, Yong; Cai, Zhiyou

    2016-10-01

    Endogenous hydrogen sulfide (H2S) may have multiple physiological functions in brain. Our previous study showed that H2S improved spatial memory impairment and decreased the production of Aβ in APP/PS1 transgenic mice. However, many of the underlying mechanisms are not still being elucidated. The aim of the present study is to investigate the neuroprotective mechanisms of H2S involving in the activity of β-secretase (BACE1), γ-secretase (PS1) and α-secretase (ADAM17). Morris water maze was used to measure the behavior change. The levels of Aβ40 and Aβ42 were quantified using colorimetric ELISA kits and immunohistochemical analysis. The levels of BACE1, PS1, ADAM17, pAkt, pp38MAPK, pERK and pJNK were tested by Western blot analysis in normal mice, APP/PS1 transgenic mice and 50μmol/kg-NaHS-treated transgenic mice. On the basis of exogenous H2S treatment, LY294002 (inhibitors of PI3K/Akt) or PD98059 (inhibitors of MAPK/ERK) was injected into lateral cerebral ventricle. The levels of BACE1, PS1 and pp38MAPK were increased and ADAM17 were decreased in the APP/PS1 transgenic mice. After intraperitoneal administration of an H2S donor (NaHS) into APP/PS1 mice, the levels of BACE1, PS1 and pp38MAPK were reduced and ADAM17 increased. The level of pp38 MAPKs, pAkt and pERK1/2 was increased in APP/PS1 transgenic mice compared with normal mice (ptransgenic mice and normal mice (p>0.05). These results demonstrated that LY294002 inhibited the effect of H2S on decreasing the BACE1 and PS1, reducing the level of Aβ and improving memory impairment in APP/PS1 transgenic mice. PD98059 had no influence on the expression of BACE1 and PS1. H2S inhibits the expression of BACE1 and PS1 by activating PI3K/Akt pathway in AD. Copyright © 2016 Institute of Pharmacology, Polish Academy of Sciences. Published by Elsevier Urban & Partner Sp. z o.o. All rights reserved.

  7. Radiation effects on Brassica seeds and seedlings

    Science.gov (United States)

    Deoli, Naresh; Hasenstein, Karl H.

    2016-07-01

    Space radiation consists of high energy charged particles and affects biological systems, but because of its stochastic, non-directional nature is difficult to replicate on Earth. Radiation damages biological systems acutely at high doses or cumulatively at low doses through progressive changes in DNA organization. These damages lead to death or cause of mutations. While radiation biology typically focuses on mammalian or human systems, little is known as to how radiation affects plants. In addition, energetic ion beams are widely used to generate new mutants in plants considering their high-LET (Linear Energy Transfer) as compared to gamma rays and X-rays. Understanding the effect of ionizing radiation on plant provides a basis for studying effects of radiation on biological systems and will help mitigate (space) radiation damage in plants. We exposed dry and imbibed Brassica rapa seeds and seedling roots to proton beams of varying qualities and compared the theoretical penetration range of different energy levels with observable growth response. We used 1, 2 and 3 MeV protons in air at the varying fluences to investigate the effect of direct irradiation on the seeds (1012 - 1015 ions/cm2) and seedlings (1013 ions/cm2). The range of protons in the tissue was calculated using Monte-Carlo based SRIM (Stopping and Range of Ions in Matter) software. The simulation and biological results indicate that ions did not penetrate the tissue of dry or hydrated seeds at all used ion energies. Therefore the entire energy was transferred to the treated tissue. Irradiated seeds were germinated vertically under dim light and roots growth was observed for two days after imbibition. The LD50 of the germination was about 2×1014 ions/cm2 and about 5×1014 ions/cm2 for imbibed and dry seeds, respectively. Since seedlings are most sensitive to gravity, the change in gravitropic behavior is a convenient means to assess radiation damage on physiological responses other than direct tissue

  8. Fabricating bio-inspired micro/nano-particles by polydopamine coating and surface interactions with blood platelets

    Energy Technology Data Exchange (ETDEWEB)

    Ye, Wei [Jiangsu Provincial Key Lab for Interventional Medical Devices, Huaiyin Institute of Technology, Huaian 223003 (China); State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022 (China); Shi, Qiang, E-mail: shiqiang@ciac.ac.cn [State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022 (China); Hou, Jianwen; Gao, Jian; Li, Chunming; Jin, Jing; Shi, Hengchong [State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022 (China); Yin, Jinghua, E-mail: yinjh@ciac.ac.cn [State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022 (China)

    2015-10-01

    Graphical abstract: The particles or particle aggregations activate the blood platelets and provide the physical adhesive sites for platelets adhesion. - Highlights: • Particles with varied sizes and surface properties were fabricated by facile polydopamine (PDA) coating on polystyrene microsphere. • The direct interaction between PDA particles and blood platelets was qualitatively investigated. • The knowledge on platelet–particle interactions provided the basic principle to select biocompatible micro/nano-particles in biomedical field. - Abstract: Although bio-inspired polydopamine (PDA) micro/nano-particles show great promise for biomedical applications, the knowledge on the interactions between micro/nano-particles and platelets is still lacking. Here, we fabricate PDA-coated micro/nano-particles and investigate the platelet–particle surface interactions. Our strategy takes the advantage of facile PDA coating on polystyrene (PS) microsphere to fabricate particles with varied sizes and surface properties, and the chemical reactivity of PDA layers to immobilize fibrinogen and bovine serum albumin to manipulate platelet activation and adhesion. We demonstrate that PS particles activate the platelets in the size-dependent manner, but PDA nanoparticles have slight effect on platelet activation; PS particles promote platelet adhesion while PDA particles reduce platelet adhesion on the patterned surface; Particles interact with platelets through activating the glycoprotein integrin receptor of platelets and providing physical sites for initial platelet adhesion. Our work sheds new light on the interaction between platelets and particles, which provides the basic principle to select biocompatible micro/nano-particles in biomedical field.

  9. Development and characterization of sub-100 ps photomultiplier tubes.

    Science.gov (United States)

    Horsfield, C J; Rubery, M S; Mack, J M; Young, C S; Herrmann, H W; Caldwell, S E; Evans, S C; Sedilleo, T J; Kim, Y H; McEvoy, A; Milnes, J S; Howorth, J; Davis, B; O'Gara, P M; Garza, I; Miller, E K; Stoeffl, W; Ali, Z

    2010-10-01

    We describe the evaluation of a microchannel plate (MCP) photomultiplier tube (PMT), incorporating a 3 μm pore MCP and constant voltage anode and cathode gaps. The use of the small pore size results in PMTs with response functions of the order of 85 ps full-width-half-maximum, while the constant electric field across the anode and cathode gaps produces a uniform response function over the entire operating range of the device. The PMT was characterized on a number of facilities and employed on gas Cherenkov detectors fielded on various deuterium tritium fuel (DT) implosions on the Omega Laser Facility at the University of Rochester. The Cherenkov detectors are part of diagnostic development to measure Gamma ray reaction history for DT implosions on the National Ignition Facility.

  10. An Antiproton Decelerator in the CERN PS Complex

    CERN Document Server

    Riunaud, J P; Baird, S A; Boillot, J; Bosser, Jacques; Brouet, M; Caspers, Friedhelm; Chanel, M; Chohan, V; Eriksson, T; Garoby, R; Giannini, R; Giovannozzi, Massimo; Gruber, J; Hémery, J Y; Koziol, Heribert; MacCaferri, R; Maury, S; Metzmacher, K D; Möhl, D; Mulder, H; Pedersen, F; Perriollat, F; Poncet, Alain; Riunaud, J P; Serre, C; Simon, Daniel Jean; Tranquille, G; Tuyn, Jan Willem Nicolaas; Williams, B; Williams, D J

    1996-01-01

    The present CERN PS low-energy antiproton complex involves 4 machines to collect, cool, decelerate and supply experiments with up to 1010 antiprotons per pulse and per hour of momenta ranging from 0.1 to 2 GeV/c. In view of a possible future physics programme requiring low energy antiprotons, mainly to carry out studies on antihydrogen, a simplified scheme providing at low cost antiprotons at 100 MeV/c has been studied. It requires only one machine, the present Antiproton Collector (AC) converted into a cooler and decelerator (Antiproton Decelerator, AD) and delivering beam to experiments in the hall of the present Antiproton Accumulator Complex (AAC) [1]. This paper describes the feasibility study of such a scheme [2].

  11. MD on Head-Tail Instability in the PS Booster

    CERN Document Server

    Kornilov, V; Mikulec, B; Aumon, S; Rumolo, G

    2013-01-01

    Machine study experiments on the coherent instabilities appearing along the magnetic ramp have been performed at the CERN PS Booster synchrotron in the week of June 11-15, 2012. The space- and time structure of the head-tail instabilities was recorded by the triggered pick-up signals due to reproducibility of the occurrence time in the shot-by-shot sense. The intensity thresholds, the absolute growth rates and the mode structure have been compared for the bunches in the single-rf and in three types of the double-rf operation. The growth rates are compared to the instantaneous synchrotron frequencies, in the cases of the large corresponding ratio the head-tail mode structure is deformed by the driving impedance. Bunch parameters measurements indicate that the PSB bunches are in the regime of very strong transverse space-charge all along the magnetic ramp.

  12. Migrating the CERN PS control system to IBM workstations

    CERN Document Server

    De Metz-Noblat, N

    1995-01-01

    The workstations used within the control system of the CERN PS accelerator complex are not produced any more. We had therefore to review the software primary used as user interface and we achieved a port to IBM workstations. We are also preparing the maintenance of this code for the next ten years with minimal staff. This implies a clear separation between general computing facilities, control system developments, and operation. In order to share our experience, we will try to summarize various aspects of this migration: - system installation principles used to speed-up error recovery time and long-term maintenance costs, - problems correlated with the coexistence of two different platforms during migration, - software problems due to the platform and operating system changes, - hidden dependencies from a specific manufacturer.

  13. Multipole Stack for the 800 MeV PS Booster

    CERN Multimedia

    1975-01-01

    The 800 MeV PS Booster had seen first beam in its 4 superposed rings in 1972, routine operation began in 1973. In the strive for ever higher beam intensities, the need for additional multipole lenses became evident. After detailed studies, the manufacture of 8 stacks of multipoles was launched in 1974. Each stack consists of 4 superposed multipoles and each multipole has 4 concentric shells. From the innermost to the outermost shell, Type A contains octupole, skew-octupole, sextupole, skew-sextupole. Type B contains skew-octupole, skew-sextupole, vertical dipole, horizontal dipole. Completion of installation in 1976 opened the way to higher beam intensities. M. Battiaz is seen here with a multipole stack and its many electrical connections.

  14. First PS magnet unit, with members of the Magnet Group.

    CERN Multimedia

    CERN PhotoLab

    1956-01-01

    Members of the Magnet Group, sitting atop the first unit of the PS combined-function magnet. The picture was taken at the Institute of Physics of Geneva University, as CERN was still a muddy construction site at that time. All these people have now retired, but all of the magnets are still pulsing away. Front row (left to right): R.Tinguely, C.Germain, G.Plass, D.Neet, B.de Raad, M.Cavallaro, K.H.Reich, G.Kuhn, J.Nilsson, C.A.Ramm, Paillard. Second row: L.Resegotti, M.Niklaus, C.J.Zilverschoon, R.Bertolotto, Marcellin, G.Brianti, P.Collet. Standing behind: B.Kuiper.

  15. Surface morphology of PS-PDMS diblock copolymer films

    DEFF Research Database (Denmark)

    Andersen, T.H.; Tougaard, S.; Larsen, N.B.

    2001-01-01

    Spin coated thin films (∼400 Å) of poly(styrene)–poly(dimethylsiloxane) (PS–PDMS) diblock copolymers have been investigated using X-ray Photoelectron Spectroscopy and Atomic Force Microscopy. Surface segregation of the poly(dimethylsiloxane) blocks was studied for five diblock copolymers which...... by use of peak shape analysis of the X-ray Photoelectron Spectra via the Tougaard Method. The amount of dimethylsiloxane in the uppermost part of the films was quantified as a function of annealing time and temperature. For annealing above the PS glass transition temperature, surface segregation...... of the dimethylsiloxane chain-ends occurs for all the studied PS–PDMS diblock copolymers. At room temperature, surface segregation takes place only when the amount of dimethylsiloxane in the diblock copolymers is small....

  16. Small-size meshless 50 ps streak tube

    Science.gov (United States)

    Ageeva, N. V.; Andreev, S. V.; Belolipetski, V. S.; Bryukhnevich, G. I.; Greenfield, D. E.; Ivanova, S. R.; Kaverin, A. M.; Khohlova, A. N.; Kuz'menko, E. A.; Levina, G. P.; Makushina, V. A.; Monastyrskiy, M. A.; Schelev, M. Ya.; Semichastnova, Z. M.; Serdyuchenko, Yu. N.; Skaballanovich, T. A.; Sokolov, V. E.

    2008-11-01

    In contrast to the conventional image intensifier with large work area, a streak image tube should possess additional important feature - the comparatively small temporal distortion at the entire work area of the photocathode. With this additional engineering restriction taken into account, a novel small-size meshless streak image tube has been developed by means of numerical optimization. The tube with 25-mm wide work area contains a pair of deflection plates to sweep the electron image along the 25 mm output phosphor screen that is separated by 100 mm from the photocathode. The electron image can be shuttered with a 300 V blanking electric pulse. Electron-optical magnification of the tube is unit; spatial resolution reaches 30 lp/mm over the entire photocathode work area; temporal resolution lies in the 20 - 50 ps range, depending on the accelerating voltage (6 - 15 kV).

  17. Psödohipoparatiroidi Tip 1A: Olgu Sunumu

    Directory of Open Access Journals (Sweden)

    Mehmet Güven

    2017-06-01

    Full Text Available Psödohipoparatiroidizm (PHPT; hedef organın parathormona (PTH yanıt vermediği kalıtımsal bir bozukluktur. Biyokimyasal olarak; hipokalsemi, hiperfosfatemi ve PTH yüksekliği ile karakterizedir. PTH uygulamasına verilen yanıt belirgin derecede düşüktür. Tip 1A, biyokimyasal özelliklere ek olarak Albright herediter osteodistrofisi (AHO olarak bilinen karakteristik somatik bir fenotipe de sahiptir. Bu fenotipin, kısa boy, yuvarlak yüz, frontal bombelik, brakidaktili, obezite özelikleri bulunmaktadır. Burada biz, hipokalsemi ve Albright herediter osteodistrofisi tanısı koyduğumuz; kalsiyum, aktif D vitamini ile tedavi ettiğimiz olgumuzu sunduk.

  18. Tree Seed Technology Training Course - Instructor's Manual

    Science.gov (United States)

    F.T. Bonner; John A. Vozzo; W.W. Elam; S.B. Land

    1994-01-01

    This manual is intended primarily to train seed collectors, seed-plant managers, seed analysts, and nursery managers, but it can serve as a resource for any training course in forest regeneration. It includes both temperate and tropical tree species of all intended uses. The manual covers the following topics: seed biology, seed collection, seed handling, seed-quality...

  19. Seed-borne pathogens and electrical conductivity of soybean seeds

    Directory of Open Access Journals (Sweden)

    Adriana Luiza Wain-Tassi

    2012-02-01

    Full Text Available Adequate procedures to evaluate seed vigor are important. Regarding the electrical conductivity test (EC, the interference in the test results caused by seed-borne pathogens has not been clarified. This research was carried out to study the influence of Phomopsis sojae (Leh. and Colletotrichum dematium (Pers. ex Fr. Grove var. truncata (Schw. Arx. fungi on EC results. Soybean seeds (Glycine max L. were inoculated with those fungi using potato, agar and dextrose (PDA medium with manitol (-1.0 MPa and incubated for 20 h at 25 °C. The colony diameter, index of mycelial growth, seed water content, occurrence of seed-borne pathogens, physiological potential of the seeds, measured by germination and vigor tests (seed germination index, cold test, accelerated aging and electrical conductivity, and seedling field emergence were determined. The contents of K+, Ca2+, and Mg2+ in the seed and in the soaking solution were also determined. A complete 2 × 4 factorial design with two seed sizes (5.5 and 6.5 mm and four treatments (control, seeds incubated without fungi, seeds incubated with Phomopsis and seeds incubated with Colletotrichum were used with eight (5.5 mm large seeds and six (6.5 mm large seeds replications. All seeds submitted to PDA medium had their germination reduced in comparison to the control seeds. This reduction was also observed when seed vigor and leached ions were considered. The presence of Phomopsis sojae fungus in soybean seed samples submitted to the EC test may be the cause of misleading results.

  20. Updating the Psoriatic Arthritis (PsA) Core Domain Set: A Report from the PsA Workshop at OMERACT 2016.

    Science.gov (United States)

    Orbai, Ana-Maria; de Wit, Maarten; Mease, Philip J; Callis Duffin, Kristina; Elmamoun, Musaab; Tillett, William; Campbell, Willemina; FitzGerald, Oliver; Gladman, Dafna D; Goel, Niti; Gossec, Laure; Hoejgaard, Pil; Leung, Ying Ying; Lindsay, Chris; Strand, Vibeke; van der Heijde, Désirée M; Shea, Bev; Christensen, Robin; Coates, Laura; Eder, Lihi; McHugh, Neil; Kalyoncu, Umut; Steinkoenig, Ingrid; Ogdie, Alexis

    2017-10-01

    To include the patient perspective in accordance with the Outcome Measures in Rheumatology (OMERACT) Filter 2.0 in the updated Psoriatic Arthritis (PsA) Core Domain Set for randomized controlled trials (RCT) and longitudinal observational studies (LOS). At OMERACT 2016, research conducted to update the PsA Core Domain Set was presented and discussed in breakout groups. The updated PsA Core Domain Set was voted on and endorsed by OMERACT participants. We conducted a systematic literature review of domains measured in PsA RCT and LOS, and identified 24 domains. We conducted 24 focus groups with 130 patients from 7 countries representing 5 continents to identify patient domains. We achieved consensus through 2 rounds of separate surveys with 50 patients and 75 physicians, and a nominal group technique meeting with 12 patients and 12 physicians. We conducted a workshop and breakout groups at OMERACT 2016 in which findings were presented and discussed. The updated PsA Core Domain Set endorsed with 90% agreement by OMERACT 2016 participants included musculoskeletal disease activity, skin disease activity, fatigue, pain, patient's global assessment, physical function, health-related quality of life, and systemic inflammation, which were recommended for all RCT and LOS. These were important, but not required in all RCT and LOS: economic cost, emotional well-being, participation, and structural damage. Independence, sleep, stiffness, and treatment burden were on the research agenda. The updated PsA Core Domain Set was endorsed at OMERACT 2016. Next steps for the PsA working group include evaluation of PsA outcome measures and development of a PsA Core Outcome Measurement Set.

  1. Effect of hygroscopic seeding on warm rain clouds – numerical study using a hybrid cloud microphysical model

    Directory of Open Access Journals (Sweden)

    N. Kuba

    2010-04-01

    Full Text Available The effect of hygroscopic seeding on warm rain clouds was examined using a hybrid cloud microphysical model combining a Lagrangian Cloud Condensation Nuclei (CCN activation model, a semi-Lagrangian droplet growth model, and an Eulerian spatial model for advection and sedimentation of droplets. This hybrid cloud microphysical model accurately estimated the effects of CCN on cloud microstructure and suggested the following conclusions for a moderate continental air mass (an air mass with a large number of background CCN. (1 Seeding can hasten the onset of surface rainfall and increase the accumulated amount of surface rainfall if the amount and radius of seeding particles are appropriate. (2 The optimal radius of monodisperse particles to increase rainfall becomes larger with the increase in the total mass of seeding particles. (3 Seeding with salt micro-powder can hasten the onset of surface rainfall and increase the accumulated amount of surface rainfall if the amount of seeding particles is sufficient. (4 Seeding by a hygroscopic flare decreases rainfall in the case of large updraft velocity (shallow convective cloud and increases rainfall slightly in the case of small updraft velocity (stratiform cloud. (5 Seeding with hygroscopic flares including ultra-giant particles (r>5 μm hastens the onset of surface rainfall but may not significantly increase the accumulated surface rainfall amount. (6 Hygroscopic seeding increases surface rainfall by two kinds of effects: the "competition effect" by which large soluble particles prevent the activation of smaller particles and the "raindrop embryo effect" in which giant soluble particles can immediately become raindrop embryos. In some cases, one of the effects works, and in other cases, both effects work, depending on the updraft velocity and the amount and size of seeding particles.

  2. Effects of (-epicatechin on the pathology of APP/PS1 transgenic mice

    Directory of Open Access Journals (Sweden)

    Yueqin eZeng

    2014-05-01

    Full Text Available Background: Alzheimer’s disease is a multifactorial disorder characterized by the progressive deterioration of neuronal networks. The clearance of Aβ from the brain and anti-inflammation are potential important strategies to prevent and treat disease. In a previous study, we demonstrated the grape seed extract (GSE could reduce brain Aβ burden and microglia activation,but which polyphenol plays a major role in these events is not known. Here we tested pharmacological effects of (-epicatechin, one principle polyphenol compound in GSE, on transgenic AD mice.Methods: APP/PS1 transgenic mice were fed with (-epicatechin diet(40mg/kg/d and curcumin diet (47mg/kg/d at 3 months of age for 9 months, the function of liver, Aβ levels in the brain and serum, AD-type neuropathology, plasma levels of inflammatory cytokines were measured.Results: Towards the end of the experiment we found long-term feeding of (- epicatechin diet was well tolerated without fatality, changes in food consumption, body weight or liver function. (-Epicatechin significantly reduced total Aβ in brain and serum by 39% and 40%, respectively, compared with control diet. Microgliosis and astrocytosis in the brain of Alzheimer’s mice were also reduced by 38% and 35%, respectively. The (-epicatechin diet did not alter learning and memory behaviors in AD mice.Conclusions: This study has provided evidence on the beneficial role of (-epicatechin in ameliorating amyloid-induced AD-like pathology in AD mice, but the impact of (-epicatechin on tau pathology is not clear, also the mechanism needs further research.

  3. First observation of o-Ps to p-Ps transition and first direct measurement of positronium hyperfine splitting with sub-THz light

    Energy Technology Data Exchange (ETDEWEB)

    Yamazaki, Takayuki, E-mail: yamazaki@icepp.s.u-tokyo.ac.jp; Miyazaki, Akira; Suehara, Taikan; Namba, Toshio; Asai, Shoji; Kobayashi, Tomio [University of Tokyo, Department of Physics, Graduate School of Science, and International Center for Elementary Particle Physics (Japan); Saito, Haruo [University of Tokyo, Graduate School of Arts and Sciences (Japan); Urushizaki, Yuichi; Ogawa, Isamu; Idehara, Toshitaka [University of Fukui, Research Center for Development of Far-Infrared Region (Japan); Sabchevski, Svilen [Bulgarian Academy of Sciences (Bulgaria)

    2012-12-15

    Positronium is an ideal system for the research of the bound state QED. The hyperfine splitting of positronium (Ps-HFS, about 203 GHz) is an important observable but all previous measurements of Ps-HFS had been measured indirectly using Zeeman splitting. There might be the unknown systematic errors on the uniformity of magnetic field. We are trying to measure Ps-HFS directly using sub-THz radiation. We developed an optical system to accumulate high power (about 10 kW) radiation in a Fabry-Perot resonant cavity and observed the positronium hyperfine transition for the first time.

  4. Polygamous particles.

    Science.gov (United States)

    Wu, Kun-Ta; Feng, Lang; Sha, Ruojie; Dreyfus, Rémi; Grosberg, Alexander Y; Seeman, Nadrian C; Chaikin, Paul M

    2012-11-13

    DNA is increasingly used as an important tool in programming the self-assembly of micrometer- and nanometer-scale particles. This is largely due to the highly specific thermoreversible interaction of cDNA strands, which, when placed on different particles, have been used to bind precise pairs in aggregates and crystals. However, DNA functionalized particles will only reach their true potential for particle assembly when each particle can address and bind to many different kinds of particles. Indeed, specifying all bonds can force a particular designed structure. In this paper, we present the design rules for multiflavored particles and show that a single particle, DNA functionalized with many different "flavors," can recognize and bind specifically to many different partners. We investigate the cost of increasing the number of flavors in terms of the reduction in binding energy and melting temperature. We find that a single 2-μm colloidal particle can bind to 40 different types of particles in an easily accessible time and temperature regime. The practical limit of ∼100 is set by entropic costs for particles to align complementary pairs and, surprisingly, by the limited number of distinct "useful" DNA sequences that prohibit subunits with nonspecific binding. For our 11 base "sticky ends," the limit is 73 distinct sequences with no unwanted overlaps of 5 bp or more. As an example of phenomena enabled by polygamous particles, we demonstrate a three-particle system that forms a fluid of isolated clusters when cooled slowly and an elastic gel network when quenched.

  5. Biological treatment to bean seeds before sowing them.

    Directory of Open Access Journals (Sweden)

    Silvano Edel Castro Mayo

    2013-09-01

    Full Text Available Even in some bibliography about the bean cultivation is recommended the use of Rhizobium which association with the cultivation is very advantageous for the fixation of the atmospheric nitrogen, as well as the employment of the antagonistic mushroom Trichoderma spp., for the control of fungous illnesses of the floor and knowing that the cultivation has its fertilization demands and of the plagues control and illnesses that are in definitive the causes that limit the yields of the cultivation, these practices are not used. We proceeded to the disinfection of bean seeds with the resulting solution of the laundry of 1Kg of Trichoderma harzianum of the stock A-34 with 2 liters of water and the mixture of this fluid with 1 Kg of Rhizobium, more a package (134 grams of Gaucho PS 70 for 45.35 kg (100 pounds of t seed. The disinfection of the seed was made by hand, 12 hour before sowing them and the results were very encouraging, so much in the delay of days in appearing the illnesses and the plagues and its low attack intensity as the increase of the yields with relationship to the not treated seed.

  6. Delay-line cables for the fast bumpers in the PS.

    CERN Multimedia

    CERN PhotoLab

    1976-01-01

    For 'continuous transfer' to the SPS, the beam accelerated in the PS is shaved off over several turns, so as to form a continuous sequence of bunches several times the length of the PS circumference. Fast bumpers, powered in a 'staircase' way, displace the PS beam stepwise towards the ejection septum. Each step lasts 2.1 microsec and the cable drums in this picture contain some of the bumper delay-lines of altogether 10 km.

  7. A DiPS+ case study: a self-healing RADIUS server

    OpenAIRE

    Michiels, Sam; Desmet, Lieven; Verbaeten, Pierre

    2004-01-01

    This report shows performance results of a RADIUS implementation using the DiPS+ software architecture. In addition it compares this implementation with a commercially available RADIUS implementation, and shows that the DiPS+ architecture differentiates between user types and request types. In fact, the DiPS+ prototype prioritizes incoming traffic based on application-specifc preferences, and allocates the available processing resources to the highest priority requests.

  8. Facile Synthesis of Mono-Dispersed Polystyrene (PS/Ag Composite Microspheres via Modified Chemical Reduction

    Directory of Open Access Journals (Sweden)

    Wen Zhu

    2013-12-01

    Full Text Available A modified method based on in situ chemical reduction was developed to prepare mono-dispersed polystyrene/silver (PS/Ag composite microspheres. In this approach; mono-dispersed PS microspheres were synthesized through dispersion polymerization using poly-vinylpyrrolidone (PVP as a dispersant at first. Then, poly-dopamine (PDA was fabricated to functionally modify the surfaces of PS microspheres. With the addition of [Ag(NH32]+ to the PS dispersion, [Ag(NH32]+ complex ions were absorbed and reduced to silver nanoparticles on the surfaces of PS-PDA microspheres to form PS/Ag composite microspheres. PVP acted both as a solvent of the metallic precursor and as a reducing agent. PDA also acted both as a chemical protocol to immobilize the silver nanoparticles at the PS surface and as a reducing agent. Therefore, no additional reducing agents were needed. The resulting composite microspheres were characterized by TEM, field emission scanning electron microscopy (FESEM, energy-dispersive X-ray spectroscopy (EDS, XRD, UV-Vis and surface-enhanced Raman spectroscopy (SERS. The results showed that Ag nanoparticles (NPs were homogeneously immobilized onto the PS microspheres’ surface in the presence of PDA and PVP. PS/Ag composite microspheres were well formed with a uniform and compact shell layer and were adjustable in terms of their optical property.

  9. Lehtmets : psühhiaatria ei ole piisavalt atraktiivne / Andres Lehtmets ; interv. Marika Kusnets

    Index Scriptorium Estoniae

    Lehtmets, Andres

    2004-01-01

    Vestlus Eesti Psühhiaatrite Seltsi aseesimehega rahva vaimsest tervisest. Samas ka sotsiaalministeeriumi rahvatervise osakonna peaspetsialisti Helja Eomoisi kommentaar: Mida teeb ministeerium rahva vaimse tervise parandamiseks?

  10. Particle cosmology

    CERN Multimedia

    CERN. Geneva

    2007-01-01

    The understanding of the Universe at the largest and smallest scales traditionally has been the subject of cosmology and particle physics, respectively. Studying the evolution of the Universe connects today's large scales with the tiny scales in the very early Universe and provides the link between the physics of particles and of the cosmos. This series of five lectures aims at a modern and critical presentation of the basic ideas, methods, models and observations in today's particle cosmology.

  11. Free fatty acid particles in protein formulations, part 2: contribution of polysorbate raw material.

    Science.gov (United States)

    Siska, Christine C; Pierini, Christopher J; Lau, Hollis R; Latypov, Ramil F; Fesinmeyer, R Matthew; Litowski, Jennifer R

    2015-02-01

    Polysorbate 20 (PS20) is a nonionic surfactant frequently used to stabilize protein biopharmaceuticals. During the development of mAb formulations containing PS20, small clouds of particles were observed in solutions stored in vials. The degree of particle formation was dependent on PS20 concentration. The particles were characterized by reversed-phase HPLC after dissolution and labeling with the fluorescent dye 1-pyrenyldiazomethane. The analysis showed that the particles consisted of free fatty acids (FFAs), with the distribution of types consistent with those found in the PS20 raw material. Protein solutions formulated with polysorbate 80, a chemically similar nonionic surfactant, showed a substantial delay in particle formation over time compared with PS20. Multiple lots of polysorbates were evaluated for FFA levels, each exhibiting differences based on polysorbate type and lot. Polysorbates purchased in more recent years show a greater distribution and quantity of FFA and also a greater propensity to form particles. This work shows that the quality control of polysorbate raw materials could play an important role in biopharmaceutical product quality. © 2014 Wiley Periodicals, Inc. and the American Pharmacists Association.

  12. Particle Physics

    CERN Document Server

    Martin, B R

    2008-01-01

    An essential introduction to particle physics, with coverage ranging from the basics through to the very latest developments, in an accessible and carefully structured text. Particle Physics: Third Edition is a revision of a highly regarded introduction to particle physics. In its two previous editions this book has proved to be an accessible and balanced introduction to modern particle physics, suitable for those students needed a more comprehensive introduction to the subject than provided by the 'compendium' style physics books. In the Third Edition the standard mod

  13. Evaluation of Moringa oleifera seed as coagulation aid for treatment ...

    African Journals Online (AJOL)

    Laboratory tests were carried out to evaluate the potentials of Moringa oleifera seed powder as a coagulation aid for removal of suspended particles in fish culture effluent. The standard jar test was used to investigate the dosage and mixing intensity required to optimize the use of the coagulant in removing of suspended ...

  14. Synthesis and Characterization of Core-shell ZrO2/PAAEM/PS Nanoparticles

    Directory of Open Access Journals (Sweden)

    Jiang XC

    2008-01-01

    Full Text Available Abstract This work demonstrates the synthesis of core-shell ZrO2/PAAEM/PS nanoparticles through a combination of sol–gel method and emulsifier-free emulsion polymerizaiton. By this method, the modified nanometer ZrO2cores were prepared by chemical modification at a molecular level of zirconium propoxide with monomer of acetoacetoxyethylmethacrylate (AAEM, and then copolymerized with vinyl monomer to form uniform-size hybrid nanoparticles with diameter of around 250 nm. The morphology, composition, and thermal stability of the core-shell particles were characterized by various techniques including transmission electron microscopy (TEM, X-ray diffractometer (XRD, Fourier transform infrared spectroscopy (FTIR, X-ray photoelectron spectroscopy (XPS, and thermal-gravimetry analyzer (TGA. The results indicate that the inorganic–organic nanocomposites exhibit good thermal stability with the maximum decomposition temperature of ~447 °C. This approach would be useful for the synthesis of other inorganic–organic nanocomposites with desired functionalities.

  15. Seed harvesting is influenced by associational effects in mixed seed neighbourhoods, not just by seed density

    Science.gov (United States)

    Ostoja, Steven M.; Schupp, Eugene W.; Durham, Susan; Klinger, Robert C.

    2013-01-01

    Rodents frequently forage in a density-dependent manner, increasing harvesting in patches with greater seed densities. Although seldom considered, seed harvesting may also depend on the species identities of other individuals in the seed neighbourhood. When the seed harvest of a focal species increases in association with another seed species, the focal species suffers from Associational Susceptibility. In contrast, if seeds of the focal species are harvested less when in association with a second species, the focal species benefits from Associational Resistance.To evaluate density dependence and associational effects among seeds in mixtures, we conducted seed removal experiments using a completely additive design patterned after a two-species competition experiment using seeds of either Achnatherum hymenoides(Indian ricegrass), Leymus cinereus (basin wildrye) or Pseudoroegneria spicata (bluebunch wheatgrass), all native perennial grasses, combined with seeds of Bromus tectorum(cheatgrass), a non-native annual grass. The experiment involved placing five fixed quantities of the native seeds mixed with five fixed quantities of B. tectorum seeds in a factorial design, resulting in 35 seed mixture combinations. The seed-eating rodent community at our study sites, in order of abundance, is composed of Peromyscus maniculatus (North American deer mouse), Dipodomys ordii (Ord's kangaroo rat) and Perognathus parvus (Great Basin pocket mouse).Native seed harvesting was density dependent, with a greater proportion of seeds being harvested as density increased. In the mixed density model, the presence of B. tectorumdid not affect harvest of any of the native species' seeds when analysed individually. However, when all three native species were analysed together, increasing quantities of B. tectorum resulted in reduced harvest of native seeds, demonstrating weak but significant Associational Resistance. In contrast, harvest of B. tectorum seeds increased

  16. Molecular characterization of plasmids pS7a and pS7b from Lactococcus lactis subsp. lactis bv. diacetylactis S50 as a base for the construction of mobilizable cloning vectors.

    Science.gov (United States)

    Strahinic, I; Kojic, M; Tolinacki, M; Fira, D; Topisirovic, L

    2009-01-01

    Strain Lactococcus lactis subsp. lactis bv. diacetylactis S50 harbours five theta-replicating plasmids (pS6, pS7a, pS7b, pS80 and pS140). The aim of this study was to characterize domains involved in the replication and conjugative mobilization of the small plasmids pS7a and pS7b, which are structurally very similar. Complete nucleotide sequences of pS7a and pS7b were determined by cloning DNA fragments of different sizes into Escherichia coli vectors. Linearized plasmids and four EcoRI fragments of the pS7a and pS7b were cloned into an origin probe vector. Constructed plasmids (pSEV10, pSK10, pISE1a and pISE1b) were able to replicate in the strain L. lactis subsp. cremoris MG1363. In addition, experiments showed that plasmids pS7a and pS7b contained oriT sequences and their conjugative transfer directly depended on the presence of pS80 in donor cells. Plasmids pS7a and pS7b contained typical lactococcal theta replication origin and repB gene that enable them to replicate in the strain L. lactis subsp. cremoris MG1363. Plasmid pS80 plays a key role in the conjugative transfer of small plasmids. Plasmids pS7a and pS7b-based derivatives could be valuable tools for genetic manipulation, studying processes of plasmid maintenance and horizontal gene transfer in lactococci.

  17. Control of seed borne diseasees in organic seed propagation

    OpenAIRE

    Borgen, Anders

    2004-01-01

    Introduction The key control measure of plant diseases in organic agriculture is crop rotation, mixed cropping and moderate fertilization. A wide range of plant diseases can be controlled or minimized in this way. However, at least one group of plant diseases, the seed borne diseases, cannot. The seed borne diseases are not transmitted through the soil, and crop rotation is therefore an insufficient tool. Mixed cropping is impractical in seed propagation, where seed purity according to the...

  18. Magnetic-seeding filtration

    Energy Technology Data Exchange (ETDEWEB)

    Depaoli, D. [Oak Ridge National Lab., TN (United States)

    1996-10-01

    This task will investigate the capabilities of magnetic-seeding filtration for the enhanced removal of magnetic and nonmagnetic particulates from liquids. This technology appies to a wide range of liquid wastes, including groundwater, process waters, and tank supernatant. Magnetic-seeding filtration can be used in several aspects of treatment, such as (1) removal of solids, particularly those in the colloidal-size range that are difficult to remove by conventional means; (2) removal of contaminants by precipitation processes; and (3) removal of contaminants by sorption processes.

  19. Restoration seed reserves for assisted gene flow within seed orchards

    Science.gov (United States)

    C.S. Echt; B.S. Crane

    2017-01-01

    Changing climate and declining forest populations imperil the future of certain forest tree species. To complement forest management and genetic conservation plans, we propose a new paradigm for seedling seed orchards: foster genetic mixing among a variety of seed sources to increase genetic diversity and adaptive potential of seed supplies used for forest restoration...

  20. Cowpea seed coat chemical analysis in relation to storage seed ...

    African Journals Online (AJOL)

    Field emergence of stored cowpea seeds in the tropical environment is a major limitation to its production, particularly in cultivars with unpigmented seed coats. To determine the storage potential of cowpea, seeds of five cultivars comprising two pigmented and three unpigmented ones were subjected to controlled ...

  1. combining high seed number and weight to improve seed yield ...

    African Journals Online (AJOL)

    ACSS

    ABSTRACT. Increasing seed size and seed weight is an important trait for trade, yield component and adaptation of chickpea. (Cicer arietinum). ..... between photosynthesis and seed number at phloem isolated nodes in soybean. Crop. Science39: 1769-1775. Carlson, J.B. 1973. Morphology. pp. 17-95. In: Wilcox, J.R. (Ed.).

  2. Cone and seed yields in white spruce seed production areas

    Science.gov (United States)

    John A. Pitcher

    1966-01-01

    The source of seed is an important consideration in the reforestation program on the National Forests in the North Central Region. Thirty-five seed production areas have been set up in the Region, along the lines proposed by the North Central Forest Experiment Station, to provide control of seed source. Red pine, white pine, shortleaf and loblolly pine, and white...

  3. Seed-feeding insects impacting globemallow seed production

    Science.gov (United States)

    Robert Hammon; Melissa Franklin

    2012-01-01

    Weevils (Anthonomus sphaeralciae Fall [Coleoptera: Curculionidae]), which attack flowers and developing seeds, can significantly impact globemallow Sphaeralcea spp. A. St.-Hil. (Malvaceae) seed production without a grower even noticing there was insect damage. This weevil damaged almost one-quarter of the flowers in a seed production field in Delta County, Colorado,...

  4. Seeds of confusion : the impact of policies on seed systems

    NARCIS (Netherlands)

    Louwaars, N.P.

    2007-01-01

    Seed is basic to crop production. Next to its importance in production, food security and rural development, seed is a key element in many debates about technology development and transfer, biodiversity, globalisation and equity. The sustainable availability of good quality seed is thus an important

  5. Lower seed rates favor seed multiplication ratio with minimal impact ...

    African Journals Online (AJOL)

    Field and laboratory studies were conducted in split plot design of three replications to determine effects of four sowing rates (50, 75, 100, 125 kg ha-1) and three row spacing (10, 20, 30 cm) on seed multiplication ratio, seed yield, and seed quality of wheat at Kulumsa and Assasa from 2012 to 2014. Results indicated that ...

  6. Utility of avocado pear seed ( Persea Americana ), mango seed ...

    African Journals Online (AJOL)

    The proximate composition and amino acid profile of three plant materials, Avocado pear seed (Persea americana), mango seed (Magifera indica) and bean seed coat (Phaseolus vulgaris) were determined. Crude protein varied from 2.0 ± 0.06% in Magifera indica to 3.73 ± 0.08% in Persea americana. Generally, the amino ...

  7. Effect of Genotypes and Seed Production Environments on Seed ...

    African Journals Online (AJOL)

    Fourteen genetically diverse sesame (Sesamum indicum L.) genotypes were grown under three diverse plant populations during 2001 and 2002 cropping seasons. Seeds Jrom each harvest were subjected to different seed quality tests and data generated were analyzed. Seed quality traits were considerably affected by ...

  8. Neural stem cells differentiated from iPS cells spontaneously regain pluripotency.

    Science.gov (United States)

    Choi, Hyun Woo; Kim, Jong Soo; Choi, Sol; Hong, Yean Ju; Kim, Min Jung; Seo, Han Geuk; Do, Jeong Tae

    2014-10-01

    Differentiated somatic cells can be reprogrammed into pluripotent stem cells by transduction of exogenous reprogramming factors. After induced pluripotent stem (iPS) cells are established, exogenous genes are silenced. In the pluripotent state, retroviral genes integrated in the host genome are kept inactive through epigenetic transcriptional regulation. In this study, we tried to determine whether exogenous genes remain silenced or are reactivated upon loss of pluripotency or on differentiation using an in vitro system. We induced differentiation of iPS cells into neural stem cells (NSCs) in vitro; the NSCs appeared morphologically indistinguishable from brain-derived NSCs and stained positive for the NSC markers Nestin and Sox2. These iPS cell-derived NSCs (iPS-NSCs) were also capable of differentiating into all three neural subtypes. Interestingly, iPS-NSCs spontaneously formed aggregates on long-term culture and showed reactivation of the Oct4-GFP marker, which was followed by the formation of embryonic stem cell-like colonies. The spontaneously reverted green fluorescent protein (GFP)-positive (iPS-NSC-GFP(+) ) cells expressed high levels of pluripotency markers (Oct4 and Nanog) and formed germline chimeras, indicating that iPS-NSC-GFP(+) cells had the same pluripotency as the original iPS cells. The reactivation of silenced exogenous genes was tightly correlated with the downregulation of DNA methyltransferases (Dnmts) during differentiation of iPS cells. This phenomenon was not observed in doxycycline-inducible iPS cells, where the reactivation of exogenous genes could be induced only by doxycycline treatment. These results indicate that pluripotency can be regained through reactivation of exogenous genes, which is associated with dynamic change of Dnmt levels during differentiation of iPS cells. © 2014 AlphaMed Press.

  9. Phosphorene-directed self-assembly of asymmetric PS-b-PMMA block copolymer for perpendicularly-oriented sub-10 nm PS nanopore arrays

    Science.gov (United States)

    Zhang, Ziming; Zheng, Lu; Khurram, Muhammad; Yan, Qingfeng

    2017-10-01

    Few-layer black phosphorus, also known as phosphorene, is a new two-dimensional material which is of enormous interest for applications, mainly in electronics and optoelectronics. Herein, we for the first time employ phosphorene for directing the self-assembly of asymmetric polystyrene-block-polymethylmethacrylate (PS-b-PMMA) block copolymer (BCP) thin film to form the perpendicular orientation of sub-10 nm PS nanopore arrays in a hexagonal fashion normal to the interface. We experimentally demonstrate that none of the PS and PMMA blocks exhibit preferential affinity to the phosphorene-modified surface. Furthermore, the perpendicularly-oriented PS nanostructures almost stay unchanged with the variation of number of layers of few-layer phosphorene nanoflakes between 15-30 layers. Differing from the neutral polymer brushes which are widely used for chemical modification of the silicon substrate, phosphorene provides a novel physical way to control the interfacial interactions between the asymmetric PS-b-PMMA BCP thin film and the silicon substrate. Based on our results, it is possible to build a new scheme for producing sub-10 nm PS nanopore arrays oriented perpendicularly to the few-layer phosphorene nanoflakes. Furthermore, the nanostructural microdomains could serve as a promising nanolithography template for surface patterning of phosphorene nanoflakes.

  10. Tissue-engineered cardiac patch seeded with human induced pluripotent stem cell derived cardiomyocytes promoted the regeneration of host cardiomyocytes in a rat model.

    Science.gov (United States)

    Sugiura, Tadahisa; Hibino, Narutoshi; Breuer, Christopher K; Shinoka, Toshiharu

    2016-12-01

    Thousands of babies are born with congenital heart defects that require surgical repair involving a prosthetic implant. Lack of growth in prosthetic grafts is especially detrimental in pediatric surgery. Cell seeded biodegradable tissue engineered grafts are a novel solution to this problem. The purpose of the present study is to evaluate the feasibility of seeding human induced pluripotent stem cell derived cardiomyocytes (hiPS-CMs) onto a biodegradable cardiac patch. The hiPS-CMs were cultured on a biodegradable patch composed of a polyglycolic acid (PGA) and a 50:50 poly (l-lactic-co-ε-caprolactone) copolymer (PLCL) for 1 week. Male athymic rats were randomly divided into 2 groups of 10 animals each: 1. hiPS-CM seeded group, and 2. Unseeded group. After culture, the cardiac patch was implanted to repair a defect with a diameter of 2 mm created in the right ventricular outflow tract (RVOT) wall. Hearts were explanted at 4 (n = 2), 8 (n = 2), and 16 (n = 6) weeks after patch implantation. Explanted patches were assessed immunohistochemically. Seeded patch explants did not stain positive for α-actinin (marker of cardiomyocytes) at the 4 week time point, suggesting that the cultured hiPS-CMs evacuated the patch in the early phase of tissue remodeling. However, after 16 weeks implantation, the area fraction of positively stained α-actinin cells was significantly higher in the seeded group than in the unseeded group (Seeded group: 6.1 ± 2.8% vs. Unseeded group: 0.95 ± 0.50%, p = 0.004), suggesting cell seeding promoted regenerative proliferation of host cardiomyocytes. Seeded hiPS-CMs were not present in the patch after 4 weeks. However, we surmise that they influenced the regeneration of host cardiomyocytes via a paracrine mechanism. Tissue-engineered hiPS-CMs seeded cardiac patches warrant further investigation for use in the repair of congenital heart diseases.

  11. Particle physics

    CERN Document Server

    Carlsmith, Duncan

    2012-01-01

    Particle Physics is the first book to connect theory and experiment in particle physics. Duncan Carlsmith provides the first accessible exposition of the standard model with sufficient mathematical depth to demystify the language of gauge theory and Feynman diagrams used by researchers in the field. Carlsmith also connects theories to past, present, and future experiments.

  12. SEEDS AND Abelmoschus esculentus (OKRA)

    African Journals Online (AJOL)

    user

    , .... process. Sample Preparations and Treatments. The kernel of Irvingia gabonensis was obtained by cracking the shell to separate out the seeds from the shell. The seeds were .... the basis of industrial processing involving water, Irvingia ...

  13. Effect of particle momentum transfer on an oblique-shock-wave/laminar-boundary-layer interaction

    Science.gov (United States)

    Teh, E.-J.; Johansen, C. T.

    2016-11-01

    Numerical simulations of solid particles seeded into a supersonic flow containing an oblique shock wave reflection were performed. The momentum transfer mechanism between solid and gas phases in the shock-wave/boundary-layer interaction was studied by varying the particle size and mass loading. It was discovered that solid particles were capable of significant modulation of the flow field, including suppression of flow separation. The particle size controlled the rate of momentum transfer while the particle mass loading controlled the magnitude of momentum transfer. The seeding of micro- and nano-sized particles upstream of a supersonic/hypersonic air-breathing propulsion system is proposed as a flow control concept.

  14. An Insight Analysis of Nano sized powder of Jackfruit Seed

    CERN Document Server

    Theivasanthi, T; 10.5101/nbe.v3i3.p163-168.

    2011-01-01

    The preparation of biodegradable nanomaterials by blending starch nanocrystals with various polymer matrices are the most active research. This work reports aspect related to nano-sized particles of jackfruit seed. This approach is simple, faster, eco-friendly, cost effective and suitable for large scale production. X-Ray Diffraction studies analyze particles size, morphology, type of starch and degree of crystallinity. The particle size is found to be 12nm, specific surface area is 625 m2g-1, contains A-type starch and 32% degree of crystallinity. A morphology index (MI) is developed from FWHM of XRD data to understand interrelationship of particle size and specific surface area. MI range is from 0.50 to 0.74. It is correlated with the particle size and specific surface area. It is observed that MI has direct relationship with particle size and an inverse relationship with specific surface area.

  15. Beam lines from Linac 1 to PS and Booster

    CERN Multimedia

    CERN PhotoLab

    1978-01-01

    View against the direction of the proton beams. The 50 MeV Linac 1 is behind the concrete wall. Its beam emerges from the hole near the centre of the picture. A switching magnet directs the beam either to the PS (to the right in the sense of the beam; original injection line), or lets it go straight on to the Booster (originally 800 MeV, now 1.4 GeV). The huge drum in the line to the Booster is a "debuncher", driven by the 200 MHz RF of the linac. It reduces the beam's momentum spread. This was the last year of Linac 1 as provider of protons to the Booster. Linac 2, nearly completed at the time of this picture, took up trial delivery at the end of 1978, and routine delivery in 1979. The beam line from Linac 2, barely visible here, can be clearly seen on 7802260. Linac 1 had a second life as an ion accelerator.

  16. The (7,7) optics at CERN PS

    CERN Document Server

    Serluca, M; Efthymiopoulos, I; Sperati, F; Sterbini, G; Tecker, F; Zisopoulos, P

    2017-01-01

    The PS lattice is composed by one hundred combinedfunction magnets, which set the bare tune of the machineto (Qh,Qv) = (6.25, 6.28). Low energy quadrupoles areused at injection to move the tune in a limited working pointarea. In particular the vertical tune is moved below 6.25 toavoid the structural resonance 8Qv= 50 coupled with spacecharge, which leads to strong losses. In view of the highdemands in terms of beam brightness for LIU and HL-LHCprojects, the interest of exploring different integer tune work-ing area started during last years. During 2016, for the firsttime, it has been possible to explore the (7,7) tune workingarea at injection using the auxiliary circuits of the combinedfunction magnets. A finite-element magnetic model, underdevelopment, has been used to predict the required currentsin order to get the desired optical parameters. In this pa-per we present the results and issues encountered duringthe Machine Development (MD) studies about the injectionin the (7,7) area along with optics and be...

  17. Injection Bump Synchronization Study for the CERN PS

    CERN Document Server

    Serluca, Maurizio; Gilardoni, Simone; CERN. Geneva. ATS Department

    2016-01-01

    In the framework of the LHC Injector Upgrade (LIU) project the CERN PS injection kinetic energy will be upgraded from 1.4 to 2 GeV. The present injection bump is made by four bumpers in Straight Section (SS) 40, 42, 43, 44 and it will be converted in a five bumpers system to allow additional flexibility in the bump shape with a reduction of the proton losses during the bump closure. The injection section SS42 has being redesigned to accommodate a new eddy current septum which will host a new bumper magnet in the same vacuum vessel due to reduced longitudinal space availability. The synchronization and amplitude variation of the power converter of the in-vacuum bumper 42 with respect to the remaining outside vacuum bumpers 40, 41, 43, 44 can lead to orbit distortion and consequent losses during injection. In this note we present the experimental results from Machine Development (MD) studies along with simulations for the present system at 1.4 GeV to quantify the acceptable orbit distortion and the performance ...

  18. Scanning SQUID sampler with 40-ps time resolution

    Science.gov (United States)

    Cui, Zheng; Kirtley, John R.; Wang, Yihua; Kratz, Philip A.; Rosenberg, Aaron J.; Watson, Christopher A.; Gibson, Gerald W.; Ketchen, Mark B.; Moler, Kathryn. A.

    2017-08-01

    Scanning Superconducting QUantum Interference Device (SQUID) microscopy provides valuable information about magnetic properties of materials and devices. The magnetic flux response of the SQUID is often linearized with a flux-locked feedback loop, which limits the response time to microseconds or longer. In this work, we present the design, fabrication, and characterization of a novel scanning SQUID sampler with a 40-ps time resolution and linearized response to periodically triggered signals. Other design features include a micron-scale pickup loop for the detection of local magnetic flux, a field coil to apply a local magnetic field to the sample, and a modulation coil to operate the SQUID sampler in a flux-locked loop to linearize the flux response. The entire sampler device is fabricated on a 2 mm × 2 mm chip and can be scanned over macroscopic planar samples. The flux noise at 4.2 K with 100 kHz repetition rate and 1 s of averaging is of order 1 mΦ0. This SQUID sampler will be useful for imaging dynamics in magnetic and superconducting materials and devices.

  19. Seed coat sculpturing in Halophila

    OpenAIRE

    Japar, Sidik Bujang; Muta, Harah Zakaria; Suzalina, Akma Awing; Nojima, Satoshi; Ogawa, Hisao

    2006-01-01

    This study furnishes information on external morphology of the seed coats of selected Halophila. Fruiting plants of Halophila beccarii, H. ovalis, H. decipiens, Halophila sp. were collected from various locations around Malaysia and including Halophila stipulacea from Mauritus. Seeds extracted from mature fruits were fixed in 2.5% glutaradehyde. Fixed seeds were washed in 0.1M Sodium cacodylate buffer at 4℃ for 10 minutes. The washing procedure was repeated three times. The seeds were dehydra...

  20. Fe{sub 3}O{sub 4}/PS magnetic nanoparticles: Synthesis, characterization and their application as sorbents of oil from waste water

    Energy Technology Data Exchange (ETDEWEB)

    Yu, Liuhua; Hao, Gazi; Gu, Junjun; Zhou, Shuai; Zhang, Ning; Jiang, Wei, E-mail: superfine_jw@126.com

    2015-11-15

    In this work, Fe{sub 3}O{sub 4}/PS composites with a rough surface and different coating rates were successfully designed and synthesized by emulsion polymerization. We carried out some comparative experiments to compare magnetic properties and oil absorption properties of the nano-magnetic materials. It had been found that several prepared groups of magnetic nanocomposites have a core–shell structure and good coating rates. These nanoparticles combined with unsinked, highly hydrophobic and superoleophilic properties. The absorption capacity of Fe{sub 3}O{sub 4}/PS composites for organic solvents and the composites could absorb diesel oil up to 2.492 times of its own weight. It is more important that the oil could be readily removed from the surfaces of nanoparticles by a simple ultrasonic treatment whereas the nanocomposites particles still kept highly hydrophobic and superoleophilic characteristics. With a combination of simple synthesis process, low density, magnetic responsibility and excellent hydrophobicity, Fe{sub 3}O{sub 4}/PS nanocomposites as a promising absorbent have great potential in the application of spilled oil recovery and environmental protection. - Graphical abstract: In this article, Fe{sub 3}O{sub 4}/PS nanoparticles present a property called selective adsorption. When the nanocomposites are added into watchglass with a layer diesel oil dyed by Sudan red for clarity on a water surface, oil is quickly absorbed in few seconds and then keeps a balance of adsorption. Owing to the presence of Fe{sub 3}O{sub 4} nanoparticles, the nanocomposites could be collected to the oil-polluted region by a magnet bar. The particles can dissolve in ethanol by ultrasonic washing. After that we can regenerate the magnetic composite materials through filtering, washing and drying, the process is showed in the following Figure. Adsorption and desorption of Fe{sub 3}O{sub 4}/PS nanoparticles. - Highlights: • We prepared Fe{sub 3}O{sub 4}/PS nanocomposites with

  1. Core-shell Li2S@Li3PS4 nanoparticles incorporated into graphene aerogel for lithium-sulfur batteries with low potential barrier and overpotential

    Science.gov (United States)

    Jiao, Zheng; Chen, Lu; Si, Jian; Xu, Chuxiong; Jiang, Yong; Zhu, Ying; Yang, Yaqing; Zhao, Bing

    2017-06-01

    Lithium sulfide as a promising cathode material not only have a high theoretical specific capacity, but also can be paired with Li-free anode material to avoid potential safety issues. However, how to prepare high electrochemical performance material is still challenge. Herein, we present a facile way to obtain high crystal quality Li2S nanomaterials with average particle size of about 55 nm and coated with Li3PS4 to form the nano-scaled core-shell Li2S@Li3PS4 composite. Then nano-Li2S@Li3PS4/graphene aerogel is prepared by a simple liquid infiltration-evaporation coating process and used directly as a composite cathode without metal substrate for lithium-sulfur batteries. Electrochemical tests demonstrate that the composite delivers a high discharge capacity of 934.4 mAh g-1 in the initial cycle and retains 485.5 mAh g-1 after 100 cycles at 0.1 C rate. In addition, the composite exhibits much lower potential barrier (∼2.40 V) and overpotential compared with previous reports, indicating that Li2S needs only a little energy to be activated. The excellent electrochemical performances could be attributed to the tiny particle size of Li2S and the superionic conducting Li3PS4 coating layer, which can shorten Li-ion and electron diffusion paths, improve the ionic conductivity, as well as retarding polysulfides dissolution into the electrolyte to some extent.

  2. Comparisons of VLP-Based ELISA, Neutralization Assays with Native HPV, and Neutralization Assays with PsV in Detecting HPV Antibody Responses in HIV-Infected Women.

    Science.gov (United States)

    Du, Ping; Brendle, Sarah; Milici, Janice; Camacho, Fabian; Zurlo, John; Christensen, Neil; Meyers, Craig

    2015-03-01

    Human papillomavirus (HPV)-associated cancers are important public health problems in HIV-infected people. Assays based on HPV virus-like particles (VLP) and pseudoviruses (PsV) are commonly used to examine HPV antibody responses in HIV-infected people, but neutralization assays with native HPV have not been utilized and a comparison of these three assays is lacking. We evaluated the agreement of assays using VLP, native HPV and PsV in detecting HPV16 and 18 antibodies in HIV-infected women. The VLP-based ELISA (VLP-ELISA) was used to detect antibody responses to HPV16 and 18 and cottontail rabbit papillomavirus (CRPV) VLP antigens. Neutralization assays with native HPV (NA-HPV) and with PsV (NA-PsV) were conducted to examine HPV16 or 18 neutralizing antibodies. Intra class correlation coefficients (ICC) and kappa coefficients were used to assess the agreements of seropositivity between the assays. The seroprevalence detected by the VLP-ELISA, NA-HPV and NA-PsV in 94 HIV-infected women was 35%, 51% and 27% for HPV16 and 14%, 44% and 21% for HPV18. Cross-reactivity between HPV16 and HPV18 was 0.35, 0.04 and 0.33 (kappa coefficients) for the VLP-ELISA, NA-HPV and NA-PsV. The agreements of seropositivity between the three assays were low. Six women who were HPV16 DNA positive were seropositive by the NA-HPV but only two were HPV16 seropositive by the VLP-ELISA or NA-PsV. One HPV18 DNA positive woman was seropositive by all three assays. Repeated tests indicated excellent reproducibility of the NA-HPV. HPV serology results vary across different assays. The NA-HPV appears to be a sensitive and reliable approach in detecting natural HPV antibodies in HIV-infected women. The NA-HPV can be applied in both HPV natural history studies and vaccine studies in HIV-infected people.

  3. Seed systems support in Kenya

    NARCIS (Netherlands)

    Munyi, Peter; Jonge, De Bram

    2015-01-01

    The threats of climate change and rising food prices have stirred renewed attention for seed and food security in Africa, inviting new thinking on the role of seed sector development in coping with these concerns. One conceptual framework that has gained attention is the Integrated Seed Sector

  4. Sexual reproduction, seeds, and seedlings

    Science.gov (United States)

    Walter T. McDonough

    1985-01-01

    Natural genetic interchange and extensive colonization of aspen by seed strongly depends upon favorable climatic and microclimatic conditions and upon human intervention. At times, in regions with the right combination of environmental conditions, there is significant L, reproduction by seed; elsewhere such establishment is rare. Seed production generally is profuse;...

  5. Germination of red alder seed.

    Science.gov (United States)

    M.A. Radwan; D.S. DeBell

    1981-01-01

    Red alder seeds were collected from six locations throughout the natural range of the species. Each seed lot was obtained from a single tree, and the seeds were used to determine germination with and without stratification treatment. Irrespective of treatment, germination varied significantly (P

  6. Characterization of amaranth seed oils

    NARCIS (Netherlands)

    Gamel, T.H.; Mesallam, A.S.; Damir, A.A.; Shekib, L.A.; Linssen, J.P.H.

    2007-01-01

    The oil fractions of Amaranthus caudatus L. and Amaranthus cruentus L. seeds were studied after different treatments of the seeds. The oil contents were 7.1 and 8.5% for raw A. caudatus L. and A. cruentus L. seeds, and consisted of 80.3¿82.3% of triacylglycerols (TAGs). Phospholipids represented

  7. The directed differentiation of human iPS cells into kidney podocytes.

    Directory of Open Access Journals (Sweden)

    Bi Song

    Full Text Available The loss of glomerular podocytes is a key event in the progression of chronic kidney disease resulting in proteinuria and declining function. Podocytes are slow cycling cells that are considered terminally differentiated. Here we provide the first report of the directed differentiation of induced pluripotent stem (iPS cells to generate kidney cells with podocyte features. The iPS-derived podocytes share a morphological phenotype analogous with cultured human podocytes. Following 10 days of directed differentiation, iPS podocytes had an up-regulated expression of mRNA and protein localization for podocyte markers including synaptopodin, nephrin and Wilm's tumour protein (WT1, combined with a down-regulation of the stem cell marker OCT3/4. In contrast to human podocytes that become quiescent in culture, iPS-derived cells maintain a proliferative capacity suggestive of a more immature phenotype. The transduction of iPS podocytes with fluorescent labeled-talin that were immunostained with podocin showed a cytoplasmic contractile response to angiotensin II (AII. A permeability assay provided functional evidence of albumin uptake in the cytoplasm of iPS podocytes comparable to human podocytes. Moreover, labeled iPS-derived podocytes were found to integrate into reaggregated metanephric kidney explants where they incorporated into developing glomeruli and co-expressed WT1. This study establishes the differentiation of iPS cells to kidney podocytes that will be useful for screening new treatments, understanding podocyte pathogenesis, and offering possibilities for regenerative medicine.

  8. Emission properties of porphyrin compounds in new polymeric PS:CBP host

    Science.gov (United States)

    Jafari, Mohammad Reza; Bahrami, Bahram

    2015-06-01

    In this study, a device with fundamental structure of ITO/PEDOT:PSS (60 nm)/PS:CBP (70 nm)/Al (150 nm) was fabricated. The electroluminescence spectrum of device designated a red shift rather than PS:CBP photoluminescence spectra. It can be suggested that the electroplex emission occurs at PS:CBP interface. By following this step, red light-emitting devices using porphyrin compounds as a red dopant in a new host material PS:CBP with a configuration of ITO/PEDOT:PSS (60 nm)/PS:CBP:porphyrin compounds(70 nm)/Al (150 nm) have been fabricated and investigated. The electroluminescent spectra of the porphyrin compounds were red-shifted as compared with the PS:CBP blend. OLED devices based on doping 3,4PtTPP and TPPNO2 in PS:CBP showed purer red emission compared with ZnTPP and CoTPP doped devices. We believe that the electroluminescence performance of OLED devices based on porphyrin compounds depends on overlaps between the absorption of the porphyrin compounds and the emission of PS:CBP.

  9. Composite Si/PS membrane pressure sensors with micro and macro ...

    Indian Academy of Sciences (India)

    Porous Silicon (PS) is a versatile material with many unique features making it viable in the field of Microelectromechanical Systems (MEMS). In this paper, we discuss the optimization of formation parameters of micro and macro PS with different porosity and thickness for use in pressure sensors. The optimized material is ...

  10. Sonication-assisted synthesis of polystyrene (PS)/organoclay nanocomposites: influence of clay content

    Science.gov (United States)

    Suresh, Kelothu; Kumar, R. Vinoth; Kumar, Manish; Jeyapriya, M.; Anbarasan, R.; Pugazhenthi, G.

    2017-06-01

    This article presents the synthesis of a series of polystyrene (PS)/organoclay nanocomposite films consisting of different contents of clay (1-7 wt%) by sonication-coupled solvent-blending technique. The prepared PS nanocomposite films were characterized using various techniques, including X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), and thermal gravimetric analysis (TGA). The XRD and TEM results revealed the formation of exfoliated nanocomposites at lower loading of organoclay (<5 wt%). The presence of various functional groups in the organoclay and PS/organoclay nanocomposite was verified by FTIR spectra. The thermal stability of PS nanocomposites was significantly improved as compared to pristine PS, which is evident from TGA analysis. When 10% mass loss was chosen as a point of reference, the thermal degradation temperature of PS nanocomposite holding 7 wt% of organoclay was found to be 30 °C more over pristine PS. The thermal kinetic parameters such as activation energy ( E a), pre-exponential factor ( A), and the order of reaction ( n) were determined by employing the Coats-Redfern model. Thermal degradation reaction mechanism of PS nanocomposites was also investigated.

  11. [Methionine metabolism regulates maintenance and differentiation of human ES/iPS cells].

    Science.gov (United States)

    Shiraki, Nobuaki; Kume, Shoen

    2015-05-01

    Embryonic stem (ES) and induced pluripotent stem (iPS) cells are pluripotent and can give rise to all cell types. ES/iPS cells have a unique transcriptional circuit that sustains the pluripotent state. These cells also possess a characteristically high rate of proliferation as well as an abbreviated G1 phase. These unique molecular properties distinguish ES and iPS cells from somatic cells. Mouse ES/iPS cells are in a high-flux metabolic state, with a high dependence on threonine catabolism. However, little is known about amino acid metabolism in human ES/iPS cells. Recently, we reported that human ES/iPS cells require high amounts of methionine (Met) and express high levels of Met metabolism enzymes (Shriaki N, et al: Cell Metabolism, 2014). Met deprivation results in a rapid decrease in intracellular S-adenosyl-methionine (SAM), triggering the activation of p53 signaling, reducing pluripotent marker gene NANOG expression, and poising human ES/iPS cells for differentiation, follow by potentiated differentiation into all three germ layers. However, when exposed to prolonged Met deprivation, the cells went to apoptosis. In this review, we explain the importance of SAM in Met metabolism and its relationship with pluripotency, cell survival, and differentiation of human ES/iPS cells.

  12. Effects of hypoxia on pluripotency in murine iPS cells.

    Science.gov (United States)

    Sugimoto, Kouji; Yoshizawa, Yuu; Yamada, Shizuka; Igawa, Kazunari; Hayashi, Yoshihiko; Ishizaki, Hidetaka

    2013-10-01

    Retroviral transduction of four transcription factors (Oct4, Sox2, Klf4 and c-Myc) or three factors, excluding c-Myc, has been shown to initiate a reprogramming process that results in the transformation of murine fibroblasts to induced pluripotent stem (iPS) cells, and there has been a rapid increase in the number of iPS cell-based preclinical trials. In this study, the effects of these transcription factors were evaluated regarding the growth and differentiation of murine iPS cells under hypoxia. Based on the results of RT-PCR and alizarin red S staining, there were no statistical differences in the growth and differentiation of iPS cells or the induction of iPS cells to osteoblasts under hypoxia between the transcription factor groups. Furthermore, the function of hypoxia inducible factors (HIFs) in murine iPS cells under hypoxia was investigated in relation to the morphology and expression of transcription factors using RT-PCR and Western blotting. The HIF-2α knockdown group exhibited a decrease in the colony size of the iPS cells. The HIF-2α or -3α knockdown group demonstrated a statistically significant decrease in the transcription factor expression compared to that observed in the control group. These results demonstrate that HIF-2α among HIFs is the most influential candidate for the maintenance of the pluripotency of murine iPS cells. Copyright © 2013 Wiley Periodicals, Inc.

  13. 7 CFR 1753.37 - Plans and specifications (P&S).

    Science.gov (United States)

    2010-01-01

    ....37 Agriculture Regulations of the Department of Agriculture (Continued) RURAL UTILITIES SERVICE... Installation of Central Office Equipment § 1753.37 Plans and specifications (P&S). (a) General. (1) Prior to... central office equipment. (2) The P&S shall specify the delivery and completion time required for each...

  14. Rahuoperatsioonide Keskuses alustab sotsiaalse ja psühholoogilise toetuse sektsioon / Merle Tihaste, Marge Sillaste

    Index Scriptorium Estoniae

    Parmak, Merle, 1968-

    2008-01-01

    Missioonidel osalevatele või pikaajalises lähetuses viibivatele kaitseväelastele ja nende peredele suunatud sotsiaalse ja psühholoogilise toetuse tagamiseks loodud sektsioonist Rahuoperatsioonide Keskuse koosseisus. Sektsiooni töömudelist, ülesannetest ja koostöövõrgustikust. Skeem: Sotsiaalse ja psühholoogilise toetuse sektsiooni töömudel ja partnerid

  15. PS Dreyer: Bakens op die pad van die wetenskap | Antonites | HTS ...

    African Journals Online (AJOL)

    PS Dreyer: Beacons on the path of science. Professor PS Dreyer is an academic who has shovsm insight and vision into several problems of the human sciences since 1951. He has identified problems, but also contributed solutions to them. In this respect his philosophy on causality and freedom is of utmost importance.

  16. Seed for change

    NARCIS (Netherlands)

    Hassena Beko, Mohammed

    2017-01-01

    Ethiopia is an agrarian country where agriculture dominates the economy, and thus agriculture is considered as an engine of growth by the government. Seed as one of the agricultural technologies, in fact, a carrier of many technologies, is critical to increasing production, but the use of quality

  17. The SEED Initiative

    Science.gov (United States)

    Teich, Carolyn R.

    2011-01-01

    Committed to fulfilling the promise of the green economy, the American Association of Community Colleges (AACC) launched the Sustainability Education and Economic Development (SEED) initiative (www.theseedcenter.org) in October 2010. The project advances sustainability and clean energy workforce development practices at community colleges by…

  18. Prosopis Africana SEEDS (OKPEYE)

    African Journals Online (AJOL)

    User

    F. U. Asoiro1,* and C. J. Ohagwu2. 1, 2, DEPARTMENT OF AGRICULTURAL AND BIORESOURCES ENGINEERING, UNIVERSITY OF NIGERIA, NSUKKA, NIGERIA .... Agricultural Engineers (ASAE) [14]. 2.2 Determination of Moisture ..... corn and Gupta and Das [43] for sunflower seed. PA has the highest bulk density at ...

  19. Preliminary Evaluation of PS300: A New Self-Lubricating High Temperature Composite Coating for Use to 800 C

    Science.gov (United States)

    Dellacorte, C.; Edmonds, B. J.

    1995-01-01

    This paper introduces PS300, a plasma sprayed, self-lubricating composite coating for use in sliding contacts at temperatures to 800 C. PS300 is a metal bonded chrome oxide coating with silver and BaF2/CaF2 eutectic solid lubricant additives. PS300 is similar to PS200, a chromium carbide based coating, which is currently being investigated for a variety of tribological applications. In pin-on-disk testing up to 650 C, PS300 exhibited comparable friction and wear properties to PS200. The PS300 matrix, which is predominantly chromium oxide rather than chromium carbide, does not require diamond grinding and polishes readily with silicon carbide abrasives greatly reducing manufacturing costs compared to PS200. It is anticipated that PS300 has potential for sliding bearing and seal applications in both aerospace and general industry.

  20. Seeding big sagebrush successfully on Intermountain rangelands

    Science.gov (United States)

    Susan E. Meyer; Thomas W. Warren

    2015-01-01

    Big sagebrush can be seeded successfully on climatically suitable sites in the Great Basin using the proper seeding guidelines. These guidelines include using sufficient quantities of high-quality seed of the correct subspecies and ecotype, seeding in late fall to mid-winter, making sure that the seed is not planted too deeply, and seeding into an environment...

  1. Tree Seed Technology Training Course: Student Outline.

    Science.gov (United States)

    Bonner, F. T.; And Others

    This manual is intended primarily to train seed collectors, seed-plant managers, seed analysts, and nursery managers, but can serve as a resource for any training course in forest regeneration. It includes both temperate and tropical tree species of all intended uses and covers the following topics: seed biology, seed collection, seed handling,…

  2. 7 CFR 201.30 - Hard seed.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Hard seed. 201.30 Section 201.30 Agriculture..., Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) FEDERAL SEED ACT FEDERAL SEED ACT REGULATIONS Labeling Vegetable Seeds § 201.30 Hard seed. The label shall show the percentage of hard seed, if...

  3. 7 CFR 201.21 - Hard seed.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Hard seed. 201.21 Section 201.21 Agriculture..., Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) FEDERAL SEED ACT FEDERAL SEED ACT REGULATIONS Labeling Agricultural Seeds § 201.21 Hard seed. The label shall show the percentage of hard seed...

  4. Trade and Transfer of Tree Seed

    DEFF Research Database (Denmark)

    Schmidt, Lars Holger

    2016-01-01

    When seed producers and seed users are geographically or functionally separated, seeds are transferred from producers to users. In market-oriented systems, transfer includes the pricing of seed, which reflects the procurement cost and seed quality. Physiological quality is documented via the seed...

  5. 7 CFR 201.15 - Weed seeds.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Weed seeds. 201.15 Section 201.15 Agriculture... REGULATIONS Labeling Agricultural Seeds § 201.15 Weed seeds. The percentage of weed seeds shall include seeds of plants considered weeds in the State into which the seed is offered for transportation or...

  6. Retinoid Uptake, Processing, and Secretion in Human iPS-RPE Support the Visual Cycle

    Science.gov (United States)

    Muñiz, Alberto; Greene, Whitney A.; Plamper, Mark L.; Choi, Jae Hyek; Johnson, Anthony J.; Tsin, Andrew T.; Wang, Heuy-Ching

    2014-01-01

    Purpose. Retinal pigmented epithelium derived from human induced pluripotent stem (iPS) cells (iPS-RPE) may be a source of cells for transplantation. For this reason, it is essential to determine the functional competence of iPS-RPE. One key role of the RPE is uptake and processing of retinoids via the visual cycle. The purpose of this study is to investigate the expression of visual cycle proteins and the functional ability of the visual cycle in iPS-RPE. Methods. iPS-RPE was derived from human iPS cells. Immunocytochemistry, RT-PCR, and Western blot analysis were used to detect expression of RPE genes lecithin-retinol acyl transferase (LRAT), RPE65, cellular retinaldehyde-binding protein (CRALBP), and pigment epithelium–derived factor (PEDF). All-trans retinol was delivered to cultured cells or whole cell homogenate to assess the ability of the iPS-RPE to process retinoids. Results. Cultured iPS-RPE expresses visual cycle genes LRAT, CRALBP, and RPE65. After incubation with all-trans retinol, iPS-RPE synthesized up to 2942 ± 551 pmol/mg protein all-trans retinyl esters. Inhibition of LRAT with N-ethylmaleimide (NEM) prevented retinyl ester synthesis. Significantly, after incubation with all-trans retinol, iPS-RPE released 188 ± 88 pmol/mg protein 11-cis retinaldehyde into the culture media. Conclusions. iPS-RPE develops classic RPE characteristics and maintains expression of visual cycle proteins. The results of this study confirm that iPS-RPE possesses the machinery to process retinoids for support of visual pigment regeneration. Inhibition of all-trans retinyl ester accumulation by NEM confirms LRAT is active in iPS-RPE. Finally, the detection of 11-cis retinaldehyde in the culture medium demonstrates the cells' ability to process retinoids through the visual cycle. This study demonstrates expression of key visual cycle machinery and complete visual cycle activity in iPS-RPE. PMID:24255038

  7. Retinoid uptake, processing, and secretion in human iPS-RPE support the visual cycle.

    Science.gov (United States)

    Muñiz, Alberto; Greene, Whitney A; Plamper, Mark L; Choi, Jae Hyek; Johnson, Anthony J; Tsin, Andrew T; Wang, Heuy-Ching

    2014-01-09

    Retinal pigmented epithelium derived from human induced pluripotent stem (iPS) cells (iPS-RPE) may be a source of cells for transplantation. For this reason, it is essential to determine the functional competence of iPS-RPE. One key role of the RPE is uptake and processing of retinoids via the visual cycle. The purpose of this study is to investigate the expression of visual cycle proteins and the functional ability of the visual cycle in iPS-RPE. iPS-RPE was derived from human iPS cells. Immunocytochemistry, RT-PCR, and Western blot analysis were used to detect expression of RPE genes lecithin-retinol acyl transferase (LRAT), RPE65, cellular retinaldehyde-binding protein (CRALBP), and pigment epithelium-derived factor (PEDF). All-trans retinol was delivered to cultured cells or whole cell homogenate to assess the ability of the iPS-RPE to process retinoids. Cultured iPS-RPE expresses visual cycle genes LRAT, CRALBP, and RPE65. After incubation with all-trans retinol, iPS-RPE synthesized up to 2942 ± 551 pmol/mg protein all-trans retinyl esters. Inhibition of LRAT with N-ethylmaleimide (NEM) prevented retinyl ester synthesis. Significantly, after incubation with all-trans retinol, iPS-RPE released 188 ± 88 pmol/mg protein 11-cis retinaldehyde into the culture media. iPS-RPE develops classic RPE characteristics and maintains expression of visual cycle proteins. The results of this study confirm that iPS-RPE possesses the machinery to process retinoids for support of visual pigment regeneration. Inhibition of all-trans retinyl ester accumulation by NEM confirms LRAT is active in iPS-RPE. Finally, the detection of 11-cis retinaldehyde in the culture medium demonstrates the cells' ability to process retinoids through the visual cycle. This study demonstrates expression of key visual cycle machinery and complete visual cycle activity in iPS-RPE.

  8. Inhibition of Ps Formation in Benzene and Cyclohexane by CH3CI and CH3Br

    DEFF Research Database (Denmark)

    Wikander, G.; Mogensen, O. E.; Pedersen, Niels Jørgen

    1983-01-01

    Positron-annihilation lifetime spectra have been measured for mixtures of CH3Cl and CH3Br in cyclohexane and of CH3Cl in benzene. The ortho-positronium (Ps) yield decreased monotonically from 38% and 43% in cyclohexane and benzene respectively to 11% in pure CH3Cl and 6% in pure CH3Br. The strength......− anions to form Ps. while it forms a bound state with the halides. X−. CH3Cl was a roughly three times weaker Ps inhibitor in benzene than in cyclohexane, which shows that CH3Cl− does not dechlorinate in times comparable to or shorter than 400–500 ps in benzene. An improved model for the explanation of Ps...

  9. iPS cell technologies: significance and applications to CNS regeneration and disease

    Science.gov (United States)

    2014-01-01

    In 2006, we demonstrated that mature somatic cells can be reprogrammed to a pluripotent state by gene transfer, generating induced pluripotent stem (iPS) cells. Since that time, there has been an enormous increase in interest regarding the application of iPS cell technologies to medical science, in particular for regenerative medicine and human disease modeling. In this review article, we outline the current status of applications of iPS technology to cell therapies (particularly for spinal cord injury), as well as neurological disease-specific iPS cell research (particularly for Parkinson’s disease and Alzheimer’s disease). Finally, future directions of iPS cell research are discussed including a) development of an accurate assay system for disease-associated phenotypes, b) demonstration of causative relationships between genotypes and phenotypes by genome editing, c) application to sporadic and common diseases, and d) application to preemptive medicine. PMID:24685317

  10. Formation of positron-atom bound states in collisions between Rydberg Ps and neutral atoms

    CERN Document Server

    Swann, A R; Deller, A; Gribakin, G F

    2016-01-01

    Predicted twenty years ago, positron binding to neutral atoms has not yet been observed experimentally. A new scheme is proposed to detect positron-atom bound states by colliding Rydberg positronium (Ps) with neutral atoms. Estimates of the charge-transfer-reaction cross section are obtained using the first Born approximation for a selection of neutral atom targets and a wide range of incident Ps energies and principal quantum numbers. We also estimate the corresponding Ps ionization cross section. The accuracy of the calculations is tested by comparison with earlier predictions for Ps charge transfer in collisions with hydrogen and antihydrogen. We describe an existing Rydberg Ps beam suitable for producing positron-atom bound states and estimate signal rates based on the calculated cross sections and realistic experimental parameters. We conclude that the proposed methodology is capable of producing such states and of testing theoretical predictions of their binding energies.

  11. Tribology and Microstructure of PS212 with a Cr2O3 Seal Coat

    Science.gov (United States)

    Sliney, Harold E.; Benoy, Patricia A.; Korenyi-Both, Andras; Dellacorte, Christopher

    1994-01-01

    PS212 is a plasma sprayed metal bonding chrome carbide coating with solid lubricant additives which has lubricating properties at temperatures up to about 900 deg C. The coating is diamond ground to achieve an acceptable tribological surface. But, as with many plasma spray coatings, PS212 is not fully-dense. In this study, a chromium oxide base seal coating is used in an attempt to seal any porosity that is open to the surface of the PS212 coating, and to study the effect of the sealant on the tribological properties of PS212. The results indicate that the seal coating reduces friction and wear when it is applied and then diamond ground leaving a thin layer of seal coating which fills in the surface pits of the PS212 coating.

  12. Biosynthesis of Au nanoparticles using cumin seed powder extract.

    Science.gov (United States)

    Sneha, Krishnamurthy; Sathishkumar, Muthuswamy; Lee, Shi Yn; Bae, Min A; Yun, Yeoung-Sang

    2011-02-01

    Cumin seed was investigated for synthesis of gold nanoparticles. Polydispersed particles were obtained at pH 3 and 30 degrees C, and the effect of temperature and pH on synthesis of gold nanoparticles was analyzed. TEM images showed that amount of platelets formed were predominant at lower temperature where as more number of monodispersed spherical particle of size 1-10 nm were perceived at high temperatures. The gold nanoparticles particles formed at higher pH were stable, uniform and spherical in shape. XPS analysis showed the presence of pure gold nanoparticles.

  13. [Effects of seed priming on vigor of Prunella vulgaris seeds].

    Science.gov (United States)

    Zhang, Xian-Xiu; Guo, Qiao-Sheng; Wang, Yan-Ru

    2008-03-01

    To select an effective way to enhance vigor of Prunella vulgaris seeds. Three population seeds were treated at the 20 degrees C and dark enviroment. Priming with 20% - 30% PEG and 200 - 400 mg x L(-1) GA3 could enhance seeds germination and vigor. Germination percentage of three population seeds treated with 0. 6% - 3.0% NaCl reduced, but they started to germinate in advance. Treated with 0.6% - 2.4% KNO3-KH2PO4, germination rate and vigor of seeds in Zijinshan and Pan' an both increased and the one in Bozhou decreased. Vigor of P. vulgaris seed treated with PEG and GA3 under proper concentration increases, while treated with KNO3-KH2PO, and NaCl low vigor seeds germination rate reduces.

  14. Structural and functional properties of hemp seed protein products.

    Science.gov (United States)

    Malomo, Sunday A; He, Rong; Aluko, Rotimi E

    2014-08-01

    The effects of pH and protein concentration on some structural and functional properties of hemp seed protein isolate (HPI, 84.15% protein content) and defatted hemp seed protein meal (HPM, 44.32% protein content) were determined. The HPI had minimum protein solubility (PS) at pH 4.0, which increased as pH was decreased or increased. In contrast, the HPM had minimum PS at pH 3.0, which increased at higher pH values. Gel electrophoresis showed that some of the high molecular weight proteins (>45 kDa) present in HPM were not well extracted by the alkali and were absent or present in low ratio in the HPI polypeptide profile. The amino acid composition showed that the isolation process increased the Arg/Lys ratio of HPI (5.52%) when compared to HPM (3.35%). Intrinsic fluorescence and circular dichroism data indicate that the HPI proteins had a well-defined structure at pH 3.0, which was lost as pH value increased. The differences in structural conformation of HPI at different pH values were reflected as better foaming capacity at pH 3.0 when compared to pH 5.0, 7.0, and 9.0. At 10 and 25 mg/mL protein concentrations, emulsions formed by the HPM had smaller oil droplet sizes (higher quality), when compared to the HPI-formed emulsions. In contrast at 50 mg/mL protein concentration, the HPI-formed emulsions had smaller oil droplet sizes (except at pH 3.0). We conclude that the functional properties of hemp seed protein products are dependent on structural conformations as well as protein concentration and pH. © 2014 Institute of Food Technologists®

  15. Intron-exon organization of the active human protein S gene PS. alpha. and its pseudogene PS. beta. : Duplication and silencing during primate evolution

    Energy Technology Data Exchange (ETDEWEB)

    Ploos van Amstel, H.; Reitsma, P.H.; van der Logt, C.P.; Bertina, R.M. (University Hospital, Leiden (Netherlands))

    1990-08-28

    The human protein S locus on chromosome 3 consists of two protein S genes, PS{alpha} and PS{beta}. Here the authors report the cloning and characterization of both genes. Fifteen exons of the PS{alpha} gene were identified that together code for protein S mRNA as derived from the reported protein S cDNAs. Analysis by primer extension of liver protein S mRNA, however, reveals the presence of two mRNA forms that differ in the length of their 5{prime}-noncoding region. Both transcripts contain a 5{prime}-noncoding region longer than found in the protein S cDNAs. The two products may arise from alternative splicing of an additional intron in this region or from the usage of two start sites for transcription. The intron-exon organization of the PS{alpha} gene fully supports the hypothesis that the protein S gene is the product of an evolutional assembling process in which gene modules coding for structural/functional protein units also found in other coagulation proteins have been put upstream of the ancestral gene of a steroid hormone binding protein. The PS{beta} gene is identified as a pseudogene. It contains a large variety of detrimental aberrations, viz., the absence of exon I, a splice site mutation, three stop codons, and a frame shift mutation. Overall the two genes PS{alpha} and PS{beta} show between their exonic sequences 96.5% homology. Southern analysis of primate DNA showed that the duplication of the ancestral protein S gene has occurred after the branching of the orangutan from the African apes. A nonsense mutation that is present in the pseudogene of man also could be identified in one of the two protein S genes of both chimpanzee and gorilla. This implicates that silencing of one of the two protein S genes must have taken place before the divergence of the three African apes.

  16. Synthesis of Cationic Core-Shell Latex Particles

    NARCIS (Netherlands)

    Dziomkina, N.; Hempenius, Mark A.; Vancso, Gyula J.

    2006-01-01

    Surfactant-free seeded (core-shell) polymerization of cationic polymer colloids is presented. Polystyrene core particles with sizes between 200 nm and 500 nm were synthesized. The number average diameter of the colloidal core particles increased with increasing monomer concentration. Cationic shells

  17. Seed priming to alleviate salinity stress in germinating seeds.

    Science.gov (United States)

    Ibrahim, Ehab A

    2016-03-15

    Salinity is one of the major abiotic stresses that affect crop production in arid and semiarid areas. Seed germination and seedling growth are the stages most sensitive to salinity. Salt stress causes adverse physiological and biochemical changes in germinating seeds. It can affect the seed germination and stand establishment through osmotic stress, ion-specific effects and oxidative stress. The salinity delays or prevents the seed germination through various factors, such as a reduction in water availability, changes in the mobilization of stored reserves and affecting the structural organization of proteins. Various techniques can improve emergence and stand establishment under salt conditions. One of the most frequently utilized is seed priming. The process of seed priming involves prior exposure to an abiotic stress, making a seed more resistant to future exposure. Seed priming stimulates the pre-germination metabolic processes and makes the seed ready for radicle protrusion. It increases the antioxidant system activity and the repair of membranes. These changes promote seed vigor during germination and emergence under salinity stress. The aim of this paper is to review the recent literature on the response of plants to seed priming under salinity stress. The mechanism of the effect of salinity on seed germination is discussed and the seed priming process is summarized. Physiological, biochemical and molecular changes induced by priming that lead to seed enhancement are covered. Plants' responses to some priming agents under salinity stress are reported based on the best available data. For a great number of crops, little information exists and further research is needed. Copyright © 2016 Elsevier GmbH. All rights reserved.

  18. Effect of Electron Seeding on Experimentally Measured Multipactor Discharge Threshold

    Science.gov (United States)

    Noland, Jonathan; Graves, Timothy; Lemon, Colby; Looper, Mark; Farkas, Alex

    2012-10-01

    Multipactor is a vacuum phenomenon in which electrons, moving in resonance with an externally applied electric field, impact material surfaces. If the number of secondary electrons created per primary electron impact averages more than unity, the resonant interaction can lead to an electron avalanche. Multipactor is a generally undesirable phenomenon, as it can cause local heating, absorb power, or cause detuning of RF circuits. In order to increase the probability of multipactor initiation, test facilities often employ various seeding sources such as radioactive sources (Cesium 137, Strontium 90), electron guns, or photon sources. Even with these sources, the voltage for multipactor initiation is not certain as parameters such as material type, RF pulse length, and device wall thickness can all affect seed electron flux and energy in critical gap regions, and hence the measured voltage threshold. This study investigates the effects of seed electron source type (e.g., photons versus beta particles), material type, gap size, and RF pulse length variation on multipactor threshold. In addition to the experimental work, GEANT4 simulations will be used to estimate the production rate of low energy electrons (< 5 keV) by high energy electrons and photons. A comparison of the experimental fluxes to the typical energetic photon and particle fluxes experienced by spacecraft in various orbits will also be made. Initial results indicate that for a simple, parallel plate device made of aluminum, there is no threshold variation (with seed electrons versus with no seed electrons) under continuous-wave RF exposure.

  19. PS2013 Satellite Workshop on Photosynthetic Light-Harvesting Systems

    Energy Technology Data Exchange (ETDEWEB)

    Niederman, Robert A. [Rutgers Univ., New Brunswick, NJ (United States); Blankenship, Robert E. [Washington Univ., St. Louis, MO (United States); Frank, Harry A. [Univ. of Connecticut, Storrs, CT (United States)

    2015-02-07

    These funds were used for partial support of the PS2013 Satellite Workshop on Photosynthetic Light-Harvesting Systems, that was held on 8-11 August, 2013, at Washington University, St. Louis, MO. This conference, held in conjunction with the 16th International Congress on Photosynthesis/St. Louis, continued a long tradition of light-harvesting satellite conferences that have been held prior to the previous six international photosynthesis congresses. In this Workshop, the basis was explored for the current interest in replacing fossil fuels with energy sources derived form direct solar radiation, coupled with light-driven electron transport in natural photosynthetic systems and how they offer a valuable blueprint for conversion of sunlight to useful energy forms. This was accomplished through sessions on the initial light-harvesting events in the biological conversion of solar energy to chemically stored energy forms, and how these natural photosynthetic processes serve as a guide to the development of robust bio-hybrid and artificial systems for solar energy conversion into both electricity or chemical fuels. Organized similar to a Gordon Research Conference, a lively, informal and collegial setting was established, highlighting the exchange of exciting new data and unpublished results from ongoing studies. A significant amount of time was set aside for open discussion and interactive poster sessions, with a special session devoted to oral presentations by talented students and postdoctoral fellows judged to have the best posters. This area of research has seen exceptionally rapid progress in recent years, with the availability of a number of antenna protein structures at atomic resolution, elucidation of the molecular surface architecture of native photosynthetic membranes by atomic force microscopy and the maturing of ultrafast spectroscopic and molecular biological techniques for the investigation and manipulation of photosynthetic systems. The conferees

  20. First direct seeding at FLASH

    Energy Technology Data Exchange (ETDEWEB)

    Maltezopoulos, Theophilos; Azima, Armin; Boedewadt, Joern; Curbis, Francesca; Delsim-Hashemi, Hossein; Drescher, Markus; Hass, Eugen; Hipp, Ulrich; Lechner, Christoph; Miltchev, Velizar; Mittenzwey, Manuel; Rehders, Marie; Roensch-Schulenburg, Juliane; Rossbach, Joerg; Schulz, Michael; Tarkeshian, Roxana; Wieland, Marek [University of Hamburg and CFEL (Germany); Ackermann, Sven; Bajt, Sasa; Duesterer, Stefan; Faatz, Bart; Felber, Matthias; Feldhaus, Josef; Honkavaara, Katja; Laarmann, Tim; Schlarb, Holger; Schreiber, Siegfried; Schroedter, Lasse; Tischer, Markus [DESY, Hamburg (Germany); Ischebeck, Rasmus [PSI, Villigen (Switzerland); Khan, Shaukat [DELTA, Dortmund (Germany)

    2013-07-01

    Direct seeding with a high-harmonic generation source can improve the spectral, temporal, and coherence properties of a free-electron-laser (FEL) and reduces intensity- and arrival-time fluctuations. In the seeding experiment at the XUV-FEL in Hamburg, FLASH, which is normally operated in the self-amplified spontaneous emission mode, the 21st harmonic of an 800 nm laser is focused into a dedicated seeding undulator. The interaction with the relativistic electrons acts as an amplifier for the seed radiation. We present the setup of the seeding section of FLASH and first experimental results.

  1. Elementary particles

    Science.gov (United States)

    Fritzsch, Harald; Heusch, Karin

    Introduction -- Electrons and atomic nuclei -- Quantum properties of atoms and particles -- The knives of Democritus -- Quarks inside atomic nuclei -- Quantum electrodynamics -- Quantum chromodynamics -- Mesons, baryons, and quarks -- Electroweak interactions -- Grand unification -- Conclusion.

  2. 7 CFR 201.33 - Seed in bulk or large quantities; seed for cleaning or processing.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Seed in bulk or large quantities; seed for cleaning or... (CONTINUED) FEDERAL SEED ACT FEDERAL SEED ACT REGULATIONS Labeling in General § 201.33 Seed in bulk or large quantities; seed for cleaning or processing. (a) In the case of seed in bulk, the information required under...

  3. Breeding for Grass Seed Yield

    DEFF Research Database (Denmark)

    Boelt, Birte; Studer, Bruno

    2010-01-01

    Seed yield is a trait of major interest for many fodder and amenity grass species and has received increasing attention since seed multiplication is economically relevant for novel grass cultivars to compete in the commercial market. Although seed yield is a complex trait and affected...... by agricultural practices as well as environmental factors, traits related to seed production reveal considerable genetic variation, prerequisite for improvement by direct or indirect selection. This chapter first reports on the biological and physiological basics of the grass reproduction system, then highlights...... important aspects and components affecting the seed yield potential and the agronomic and environmental aspects affecting the utilization and realization of the seed yield potential. Finally, it discusses the potential of plant breeding to sustainably improve total seed yield in fodder and amenity grasses....

  4. Auroral particles

    Science.gov (United States)

    Evans, David S.

    1987-01-01

    The problems concerning the aurora posed prior to the war are now either solved in principle or were restated in a more fundamental form. The pre-war hypothesis concerning the nature of the auroral particles and their energies was fully confirmed, with the exception that helium and oxygen ions were identified as participating in the auroral particle precipitation in addition to the protons. The nature of the near-Earth energization processes affecting auroral particles was clarified. Charged particle trajectories in various electric field geometries were modeled. The physical problems have now moved from determining the nature and geometry of the electric fields, which accelerate charged particles near the Earth, to accounting for the existence of these electric fields as a natural consequence of the solar wind's interaction with Earth. Ultimately the reward in continuing the work in auroral and magnetospheric particle dynamics will be a deeper understanding of the subtleties of classical electricity and magnetism as applied to situations not blessed with well-defined and invariant geometries.

  5. Fluidized-Bed Cleaning of Silicon Particles

    Science.gov (United States)

    Rohatgi, Naresh K.; Hsu, George C.

    1987-01-01

    Fluidized-bed chemical cleaning process developed to remove metallic impurities from small silicon particles. Particles (250 micrometer in size) utilized as seed material in silane pyrolysis process for production of 1-mm-size silicon. Product silicon (1 mm in size) used as raw material for fabrication of solar cells and other semiconductor devices. Principal cleaning step is wash in mixture of hydrochloric and nitric acids, leaching out metals and carrying them away as soluble chlorides. Particles fluidized by cleaning solution to assure good mixing and uniform wetting.

  6. Particle size distribution instrument. Topical report 13

    Energy Technology Data Exchange (ETDEWEB)

    Okhuysen, W.; Gassaway, J.D.

    1995-04-01

    The development of an instrument to measure the concentration of particles in gas is described in this report. An in situ instrument was designed and constructed which sizes individual particles and counts the number of occurrences for several size classes. Although this instrument was designed to detect the size distribution of slag and seed particles generated at an experimental coal-fired magnetohydrodynamic power facility, it can be used as a nonintrusive diagnostic tool for other hostile industrial processes involving the formation and growth of particulates. Two of the techniques developed are extensions of the widely used crossed beam velocimeter, providing simultaneous measurement of the size distribution and velocity of articles.

  7. Longitudinal space charge assisted echo seeding of a free-electron laser with laser-spoiler noise suppression

    Directory of Open Access Journals (Sweden)

    Kirsten Hacker

    2014-09-01

    Full Text Available Seed lasers are employed to improve the temporal coherence of free-electron laser (FEL light. However, when these seed pulses are short relative to the particle bunch, the noisy, temporally incoherent radiation from the unseeded electrons can overwhelm the coherent, seeded radiation. In this paper, a technique to seed a particle bunch with an external laser is presented in which a new mechanism to improve the contrast between coherent and incoherent free electron laser radiation is employed together with a novel, simplified echo-seeding method. The concept relies on a combination of longitudinal space charge wakes and an echo-seeding technique to make a short, coherent pulse of FEL light together with noise background suppression. Several different simulation codes are used to illustrate the concept with conditions at the soft x-ray free-electron laser in Hamburg, FLASH.

  8. An HDAC2-TET1 switch at distinct chromatin regions significantly promotes the maturation of pre-iPS to iPS cells.

    Science.gov (United States)

    Wei, Tingyi; Chen, Wen; Wang, Xiukun; Zhang, Man; Chen, Jiayu; Zhu, Songcheng; Chen, Long; Yang, Dandan; Wang, Guiying; Jia, Wenwen; Yu, Yangyang; Duan, Tao; Wu, Minjuan; Liu, Houqi; Gao, Shaorong; Kang, Jiuhong

    2015-06-23

    The maturation of induced pluripotent stem cells (iPS) is one of the limiting steps of somatic cell reprogramming, but the underlying mechanism is largely unknown. Here, we reported that knockdown of histone deacetylase 2 (HDAC2) specifically promoted the maturation of iPS cells. Further studies showed that HDAC2 knockdown significantly increased histone acetylation, facilitated TET1 binding and DNA demethylation at the promoters of iPS cell maturation-related genes during the transition of pre-iPS cells to a fully reprogrammed state. We also found that HDAC2 competed with TET1 in the binding of the RbAp46 protein at the promoters of maturation genes and knockdown of TET1 markedly prevented the activation of these genes. Collectively, our data not only demonstrated a novel intrinsic mechanism that the HDAC2-TET1 switch critically regulates iPS cell maturation, but also revealed an underlying mechanism of the interplay between histone acetylation and DNA demethylation in gene regulation. © The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

  9. Physiological quality and seed respiration of primed Jatropha curcas seeds

    Directory of Open Access Journals (Sweden)

    Micheli Angelica Horbach

    2017-11-01

    Full Text Available ABSTRACT Seed deterioration is a natural and irreversible process. Nevertheless, seed priming with water and antioxidants can minimize oxidative damage in oilseeds, resulting in attenuation of seed deterioration. The objective of this assay was to evaluate seed priming on respiratory activity of Jatropha curcas submitted to accelerated aging. Seeds from two provenances (Janauba and Pedro J. Caballero were submitted to three priming treatments (control, immersion in deionized water, and with 750 µmol L-1 of ascorbic acid and treated for accelerated aging at 41 °C for 72 h. The results showed that the priming of J. curcas seeds promoted tolerance to accelerated aging. Primed seeds, with ascorbic acid from Janauba and deionized water from Pedro J. Caballero, resulted in a higher percentage of normal seedlings, and increased germination speed index and seed respiration. The decline of physiological quality of J. curcas seeds after accelerated aging is directly associated with a reduction in respiratory activity that is related to seed moisture content.

  10. Empty seeds are not always bad: simultaneous effect of seed emptiness and masting on animal seed predation.

    Science.gov (United States)

    Perea, Ramón; Venturas, Martin; Gil, Luis

    2013-01-01

    Seed masting and production of empty seeds have often been considered independently as different strategies to reduce seed predation by animals. Here, we integrate both phenomena within the whole assemblage of seed predators (both pre and post-dispersal) and in two contrasting microsites (open vs. sheltered) to improve our understanding of the factors controlling seed predation in a wind-dispersed tree (Ulmus laevis). In years with larger crop sizes more avian seed predators were attracted with an increase in the proportion of full seeds predated on the ground. However, for abundant crops, the presence of empty seeds decreased the proportion of full seeds predated. Empty seeds remained for a very long period in the tree, making location of full seeds more difficult for pre-dispersal predators and expanding the overall seed drop period at a very low cost (in dry biomass and allocation of C, N and P). Parthenocarpy (non-fertilized seeds) was the main cause of seed emptiness whereas seed abortion was produced in low quantity. These aborted seeds fell prematurely and, thus, could not work as deceptive seeds. A proportion of 50% empty seeds significantly reduced ground seed predation by 26%. However, a high rate of parthenocarpy (beyond 50% empty seeds) did not significantly reduce seed predation in comparison to 50% empty seeds. We also found a high variability and unpredictability in the production of empty seeds, both at tree and population level, making predator deception more effective. Open areas were especially important to facilitate seed survival since rodents (the main post-dispersal predators) consumed seeds mostly under shrub cover. In elm trees parthenocarpy is a common event that might work as an adaptive strategy to reduce seed predation. Masting per se did not apparently reduce the overall proportion of seeds predated in this wind-dispersed tree, but kept great numbers of seeds unconsumed.

  11. Empty seeds are not always bad: simultaneous effect of seed emptiness and masting on animal seed predation.

    Directory of Open Access Journals (Sweden)

    Ramón Perea

    Full Text Available Seed masting and production of empty seeds have often been considered independently as different strategies to reduce seed predation by animals. Here, we integrate both phenomena within the whole assemblage of seed predators (both pre and post-dispersal and in two contrasting microsites (open vs. sheltered to improve our understanding of the factors controlling seed predation in a wind-dispersed tree (Ulmus laevis. In years with larger crop sizes more avian seed predators were attracted with an increase in the proportion of full seeds predated on the ground. However, for abundant crops, the presence of empty seeds decreased the proportion of full seeds predated. Empty seeds remained for a very long period in the tree, making location of full seeds more difficult for pre-dispersal predators and expanding the overall seed drop period at a very low cost (in dry biomass and allocation of C, N and P. Parthenocarpy (non-fertilized seeds was the main cause of seed emptiness whereas seed abortion was produced in low quantity. These aborted seeds fell prematurely and, thus, could not work as deceptive seeds. A proportion of 50% empty seeds significantly reduced ground seed predation by 26%. However, a high rate of parthenocarpy (beyond 50% empty seeds did not significantly reduce seed predation in comparison to 50% empty seeds. We also found a high variability and unpredictability in the production of empty seeds, both at tree and population level, making predator deception more effective. Open areas were especially important to facilitate seed survival since rodents (the main post-dispersal predators consumed seeds mostly under shrub cover. In elm trees parthenocarpy is a common event that might work as an adaptive strategy to reduce seed predation. Masting per se did not apparently reduce the overall proportion of seeds predated in this wind-dispersed tree, but kept great numbers of seeds unconsumed.

  12. Separation of plastics by froth flotation. The role of size, shape and density of the particles.

    Science.gov (United States)

    Pita, Fernando; Castilho, Ana

    2017-02-01

    Over the last few years, new methods for plastic separation in mining have been developed. Froth flotation is one of these techniques, which is based on hydrophobicity differences between particles. Unlike minerals, most of the plastics are naturally hydrophobic, thus requiring the addition of chemicals that promote the selective wettability of one of its components, for a flotation separation. The floatability of six granulated post-consumer plastic - Polystyrene (PS), Polymethyl methacrylate (PMMA), Polyethylene Terephthalate (PET-S, PET-D) and Polyvinyl Chloride (PVC-M, PVC-D) - in the presence of tannic acid (wetting agent), and the performance of the flotation separation of five bi-component plastic mixtures - PS/PMMA, PS/PET-S, PS/PET-D, PS/PVC-M and PS/PVC-D - were evaluated. Moreover, the effect of the contact angle, density, size and shape of the particles was also analysed. Results showed that all plastics were naturally hydrophobic, with PS exhibiting the highest floatability. The contact angle and the flotation recovery of six plastics decreased with increasing tannic acid concentration, occurring depression of plastics at very low concentrations. Floatability differed also with the size and shape of plastic particles. For regular-shaped plastics (PS, PMMA and PVC-D) floatability decreased with the increase of particle size, while for lamellar-shaped particles (PET-D) floatability was slightly greater for coarser particles. Thus, plastic particles with small size, lamellar shape and low density present a greater floatability. The quality of separation varied with the mixture type, depending not only on the plastics hydrophobicity, but also on the size, density and shape of the particles, i.e. the particle weight. Flotation separation of plastics can be enhanced by differences in hydrophobicity. In addition, flotation separation improves if the most hydrophobic plastic, that floats, has a lamellar shape and lower density and if the most hydrophilic

  13. Chemical phase analysis of seed mediated synthesized anisotropic silver nanoparticles

    Energy Technology Data Exchange (ETDEWEB)

    Bharti, Amardeep, E-mail: abharti@pu.ac.in; Goyal, Navdeep [Department of Physics, Panjab University, Chandigarh, INDIA-160014 (India); Singh, Suman; Singla, M. L. [Agrionics, Central Scientific Instruments Organization, CSIR, Chandigarh, INDIA-160030 (India)

    2015-08-28

    Noble-metal nanoparticles are of great interest because of its broad applications almost in every stream (i.e. biology, chemistry and engineering) due to their unique size/shape dependant properties. In this paper, chemical phase of seed mediated synthesized anisotropic silver nanoparticle (AgNPs) has been investigated via fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA). These nanaoparticles were synthesized by seed-growth method controlled by urea and dextrose results to highly stable 12-20 nm particle size revealed by zeta potential and transmission electron microscopy (TEM)

  14. Wet chemical synthesis of gold nanoparticles using silver seeds: a shape control from nanorods to hollow spherical nanoparticles

    Science.gov (United States)

    Xu, Zhi-Chuan; Shen, Cheng-Min; Xiao, Cong-Wen; Yang, Tian-Zhong; Zhang, Huai-Ruo; Li, Jian-Qi; Li, Hu-Lin; Gao, Hong-Jun

    2007-03-01

    A seed-mediated method was employed here for CTAB-assisted gold nanoparticle growth. 3-4 nm silver aqueous colloid was stabilized by sodium citrate and used as seed solution to initial gold particle growth. The concentration of seed solution was calculated based on its relationship with silver atom concentration and seed particle statistical mean volume. It was found that there is a maximum seed concentration of 8.57 × 10-12 M (~25 µl 0.343 × 10-8 M seed solution added) in 10 ml 2.5 × 10-4 M HAuCl4 growth solution for growth of rodlike particles. Below this seed amount, the aspect ratio of nanorods could be controlled by varying the silver seed amount, i.e. nanorods with aspect ratio ~18.9 were obtained when the seed concentration in the growth solution was 0.343 × 10-12 M by adding 1 µl 0.343 × 10-8 M silver seed solution and nanorods with aspect ratio ~9.69 were obtained when the seed concentration in the growth solution was 1.715 × 10-12 M by adding 5 µl 0.343 × 10-8 M silver seed solution. As the seed concentration in the growth solution was more than 8.58 × 10-12 M (25 µl 0.343 × 10-8 M silver seed solution was added), there were no rodlike particles formed but spherical ones instead. These spheres were further studied by TEM and found to all be hollow structures. It was suggested that there were probably two different nucleation processes for growth of nanorods and spheres. For hollow spheres, the reaction between Ag seeds and Au ions formed hollow structures based on the Ag particle template effect. Then further growth of Au on these hollow structures produced hollow gold nanospheres. For nanorods, due to the very low concentration of silver seed (molar ratio of Ag seed: Au = 3.426 × 10-8), the growth process here probably was started by silver-induced Au nucleation, in which reduction of gold ions by silver resulted in small gold clusters. These gold clusters further grew up into nanoparticles and nanorods in the presence of CTAB.

  15. Early temporal short-term memory deficits in double transgenic APP/PS1 mice.

    Science.gov (United States)

    Lagadec, Saioa; Rotureau, Lolita; Hémar, Agnès; Macrez, Nathalie; Delcasso, Sebastien; Jeantet, Yannick; Cho, Yoon H

    2012-01-01

    We tested single APP (Tg2576) transgenic, PS1 (PS1dE9) transgenic, and double APP/PS1 transgenic mice at 3 and 6 months of age on the acquisition of a hippocampal-dependent operant "differential reinforcement of low rate schedule" (DRL) paradigm. In this task mice are required to wait for at least 10 seconds (DRL-10s) between 2 consecutive nose poke responses. Our data showed that while single APP and PS1 transgene expression did not affect DRL learning and performance, mice expressing double APP/PS1 transgenes were impaired in the acquisition of DRL-10s at 6 months, but not at 3 months of age. The same impaired double transgenic mice, however, were perfectly capable of normal acquisition of signaled DRL-10s (SDRL-10s) task, a hippocampal-independent task, wherein mice were required to emit responses when the end of the 10-second delay was signaled by a lighting of the chamber. The age-dependent and early deficits of APP/PS1 mice suggest that the appetitive DRL paradigm is sensitive to the amyloid pathology present in double APP/PS1 mice, and that this mouse line represents a good model with which to study the efficacy of therapeutic strategies against Alzheimer's disease. Copyright © 2012 Elsevier Inc. All rights reserved.

  16. Reprogramming in vivo produces teratomas and iPS cells with totipotency features.

    Science.gov (United States)

    Abad, María; Mosteiro, Lluc; Pantoja, Cristina; Cañamero, Marta; Rayon, Teresa; Ors, Inmaculada; Graña, Osvaldo; Megías, Diego; Domínguez, Orlando; Martínez, Dolores; Manzanares, Miguel; Ortega, Sagrario; Serrano, Manuel

    2013-10-17

    Reprogramming of adult cells to generate induced pluripotent stem cells (iPS cells) has opened new therapeutic opportunities; however, little is known about the possibility of in vivo reprogramming within tissues. Here we show that transitory induction of the four factors Oct4, Sox2, Klf4 and c-Myc in mice results in teratomas emerging from multiple organs, implying that full reprogramming can occur in vivo. Analyses of the stomach, intestine, pancreas and kidney reveal groups of dedifferentiated cells that express the pluripotency marker NANOG, indicative of in situ reprogramming. By bone marrow transplantation, we demonstrate that haematopoietic cells can also be reprogrammed in vivo. Notably, reprogrammable mice present circulating iPS cells in the blood and, at the transcriptome level, these in vivo generated iPS cells are closer to embryonic stem cells (ES cells) than standard in vitro generated iPS cells. Moreover, in vivo iPS cells efficiently contribute to the trophectoderm lineage, suggesting that they achieve a more plastic or primitive state than ES cells. Finally, intraperitoneal injection of in vivo iPS cells generates embryo-like structures that express embryonic and extraembryonic markers. We conclude that reprogramming in vivo is feasible and confers totipotency features absent in standard iPS or ES cells. These discoveries could be relevant for future applications of reprogramming in regenerative medicine.

  17. Integration-Free iPS Cells Engineered Using Human Artificial Chromosome Vectors

    Science.gov (United States)

    Hiratsuka, Masaharu; Uno, Narumi; Ueda, Kana; Kurosaki, Hajime; Imaoka, Natsuko; Kazuki, Kanako; Ueno, Etsuya; Akakura, Yutaro; Katoh, Motonobu; Osaki, Mitsuhiko; Kazuki, Yasuhiro; Nakagawa, Masato; Yamanaka, Shinya; Oshimura, Mitsuo

    2011-01-01

    Human artificial chromosomes (HACs) have unique characteristics as gene-delivery vectors, including episomal transmission and transfer of multiple, large transgenes. Here, we demonstrate the advantages of HAC vectors for reprogramming mouse embryonic fibroblasts (MEFs) into induced pluripotent stem (iPS) cells. Two HAC vectors (iHAC1 and iHAC2) were constructed. Both carried four reprogramming factors, and iHAC2 also encoded a p53-knockdown cassette. iHAC1 partially reprogrammed MEFs, and iHAC2 efficiently reprogrammed MEFs. Global gene expression patterns showed that the iHACs, unlike other vectors, generated relatively uniform iPS cells. Under non-selecting conditions, we established iHAC-free iPS cells by isolating cells that spontaneously lost iHAC2. Analyses of pluripotent markers, teratomas and chimeras confirmed that these iHAC-free iPS cells were pluripotent. Moreover, iHAC-free iPS cells with a re-introduced HAC encoding Herpes Simplex virus thymidine kinase were eliminated by ganciclovir treatment, indicating that the HAC safeguard system functioned in iPS cells. Thus, the HAC vector could generate uniform, integration-free iPS cells with a built-in safeguard system. PMID:21998730

  18. Inhibition of the integrin signal constitutes a mouse iPS cell niche.

    Science.gov (United States)

    Higuchi, Sayaka; Yoshina, Sawako; Mitani, Shohei

    2016-09-01

    Stem cells are regulated by their surrounding microenvironments, called niche, such as cell-cell interaction and extracellular matrix. Classically, feeder cells as a niche have been used in the culture of iPS cells from both the mouse and the human. However, the regulation mechanism of stem cells by feeder cells as a niche still have been partially unclear. In this study, we used three murine iPS cell lines, iPS-MEF-Ng-20D-17, iPS-MEF-Ng-178B-5 and iPS-MEF-Fb/Ng-440A-3, which were generated by different reprogramming methods. In general, these cell lines commonly need the feeder cells as a niche to culture. Recently, the effect of substrate stiffness is known in stem cell study. First, we focused on the mechanical properties of feeder cells, and then we speculated that feeder-less culture might be made possible by using molecules in place of the mechanical properties of the niche. Finally, we found that the combination of disintegrin (echistatin) and 2i (GSK3 inhibitor and MEK inhibitor) is a sufficient condition for three murine iPS culture. This novel method of mimicking the murine iPS cell niche may be useful to understand signaling pathways to maintain the pluripotency of stem cells. © 2016 The Authors. Development, Growth & Differentiation published by John Wiley & Sons Australia, Ltd on behalf of Japanese Society of Developmental Biologists.

  19. [Effects of Different Culture Systems on the Hematopoietic Differentiation Ability of iPS Cells].

    Science.gov (United States)

    Fan, Di; He, Wen-Yin; Niu, Xiao-Hua; Ou, Zhan-Hui; Chen, Yu-Chang; Sun, Xiao-Fang

    2016-12-01

    To investigate the in vitro effects of different culture systems on hematopoietic differentiation ability of induced pluripotent stem (iPS) cells. Two culture systems including E8 and mTESR(freeder-free medium), and the classical ES culture medium were chosen for culture of iPS cells. The iPS cells maintaining in above mentioning culcure systems were co-cultured with OP9 cells(murine bone marrow stromal cells) in vitro to be induced to differentiate into hematopoietic stem/progenitor cells. Flow cytometry and real-time quantitative PCR were used to detect the expression of specific hematopoietic markers and the effects of different culture systems on the differentiation of iPS in vitro. iPS cultured in the 3 selected medium could be differentiated into hematopoietic stem cells. Efficiency of hematopoietic differentiation was up to 28.4% in classical ES culture system, which was significantly higher than that in E8 and mTESR system. Under the co-culture with OP9, iPS can differentiate into hematopoietic stem/progenitor cells, which shows higher efficiency when iPS maintained in the ES medium.

  20. Morphological Evolution of Block Copolymer Particles: Effect of Solvent Evaporation Rate on Particle Shape and Morphology.

    Science.gov (United States)

    Shin, Jae Man; Kim, YongJoo; Yun, Hongseok; Yi, Gi-Ra; Kim, Bumjoon J

    2017-02-28

    Shape and morphology of polymeric particles are of great importance in controlling their optical properties or self-assembly into unusual superstructures. Confinement of block copolymers (BCPs) in evaporative emulsions affords particles with diverse structures, including prolate ellipsoids, onion-like spheres, oblate ellipsoids, and others. Herein, we report that the evaporation rate of solvent from emulsions encapsulating symmetric polystyrene-b-polybutadiene (PS-b-PB) determines the shape and internal nanostructure of micron-sized BCP particles. A distinct morphological transition from the ellipsoids with striped lamellae to the onion-like spheres was observed with decreasing evaporation rate. Experiments and dissipative particle dynamics (DPD) simulations showed that the evaporation rate affected the organization of BCPs at the particle surface, which determined the final shape and internal nanostructure of the particles. Differences in the solvent diffusion rates in PS and PB at rapid evaporation rates induced alignment of both domains perpendicular to the particle surface, resulting in ellipsoids with axial lamellar stripes. Slower evaporation rates provided sufficient time for BCP organization into onion-like structures with PB as the outermost layer, owing to the preferential interaction of PB with the surroundings. BCP molecular weight was found to influence the critical evaporation rate corresponding to the morphological transition from ellipsoid to onion-like particles, as well as the ellipsoid aspect ratio. DPD simulations produced morphologies similar to those obtained from experiments and thus elucidated the mechanism and driving forces responsible for the evaporation-induced assembly of BCPs into particles with well-defined shapes and morphologies.

  1. Seeds of the Future

    CERN Multimedia

    CERN. Geneva

    2015-01-01

    Five of the global issues most frequently debated today are the decline of biodiversity in general and of agrobiodiversity in particular, climate change, hunger and malnutrition, poverty and water. These issues are connected with each other, and should be dealt with as such. Most of our food comes from seeds (even when we eat meat, we indirectly eat plants, which come from seeds) and food affects our health. The evolution of plant breeding, the science which is responsible for the type and the diversity of seed that farmers plant, and hence for the diversity of food that we eat, helps us understand how agrobiodiversity has decreased. An agro-ecological model of agriculture could be solution to the most important problems affecting the planet, but is often criticized for not being able to produce enough food for a growing population casting doubts on whether food security and food safety can be compatible objectives. Participatory and evolutionary plant breeding, while benefiting from advances in molecular g...

  2. A family of small cyclic amphipathic peptides (SCAmpPs) genes in citrus.

    Science.gov (United States)

    Belknap, William R; McCue, Kent F; Harden, Leslie A; Vensel, William H; Bausher, Michael G; Stover, Ed

    2015-04-16

    Citrus represents a crop of global importance both in economic impact and significance to nutrition. Citrus production worldwide is threatened by the disease Huanglongbing (HLB), caused by the phloem-limited pathogen Candidatus Liberibacter spp.. As a source of stable HLB-resistance has yet to be identified, there is considerable interest in characterization of novel disease-associated citrus genes. A gene family of Small Cyclic Amphipathic Peptides (SCAmpPs) in citrus is described. The citrus genomes contain 100-150 SCAmpPs genes, approximately 50 of which are represented in the citrus EST database. These genes encode small ~50 residue precursor proteins that are post-translationally processed, releasing 5-10 residue cyclic peptides. The structures of the SCAmpPs genes are highly conserved, with the small coding domains interrupted by a single intron and relatively extended untranslated regions. Some family members are very highly transcribed in specific citrus tissues, as determined by representation in tissue-specific cDNA libraries. Comparison of the ESTs of related SCAmpPs revealed an unexpected evolutionary profile, consistent with targeted mutagenesis of the predicted cyclic peptide domain. The SCAmpPs genes are displayed in clusters on the citrus chromosomes, with apparent association with receptor leucine-rich repeat protein arrays. This study focused on three SCAmpPs family members with high constitutive expression in citrus phloem. Unexpectedly high sequence conservation was observed in the promoter region of two phloem-expressed SCAmpPs that encode very distinct predicted cyclic products. The processed cyclic product of one of these phloem SCAmpPs was characterized by LC-MS-MS analysis of phloem tissue, revealing properties consistent with a K(+) ionophore. The SCAmpPs amino acid composition, protein structure, expression patterns, evolutionary profile and chromosomal distribution are consistent with designation as ribosomally synthesized defense

  3. Delayed amyloid plaque deposition and behavioral deficits in outcrossed AβPP/PS1 mice.

    Science.gov (United States)

    Couch, Brian A; Kerrisk, Meghan E; Kaufman, Adam C; Nygaard, Haakon B; Strittmatter, Stephen M; Koleske, Anthony J

    2013-04-15

    Alzheimer's disease (AD) is a progressive neurodegenerative dementia characterized by amyloid plaque accumulation, synapse/dendrite loss, and cognitive impairment. Transgenic mice expressing mutant forms of amyloid-β precursor protein (AβPP) and presenilin-1 (PS1) recapitulate several aspects of this disease and provide a useful model system for studying elements of AD progression. AβPP/PS1 mice have been previously shown to exhibit behavioral deficits and amyloid plaque deposition between 4-9 months of age. We crossed AβPP/PS1 animals with mice of a mixed genetic background (C57BL/6 × 129/SvJ) and investigated the development of AD-like features in the resulting outcrossed mice. The onset of memory-based behavioral impairment is delayed considerably in outcrossed AβPP/PS1 mice relative to inbred mice on a C57BL/6 background. While inbred AβPP/PS1 mice develop deficits in radial-arm water maze performance and novel object recognition as early as 8 months, outcrossed AβPP/PS1 mice do not display defects until 18 months. Within the forebrain, we find that inbred AβPP/PS1 mice have significantly higher amyloid plaque burden at 12 months than outcrossed AβPP/PS1 mice of the same age. Surprisingly, inbred AβPP/PS1 mice at 8 months have low plaque burden, suggesting that plaque burden alone cannot explain the accompanying behavioral deficits. Analysis of AβPP processing revealed that elevated levels of soluble Aβ correlate with the degree of behavioral impairment in both strains. Taken together, these findings suggest that animal behavior, amyloid plaque deposition, and AβPP processing are sensitive to genetic differences between mouse strains. Copyright © 2012 Wiley Periodicals, Inc.

  4. Physicochemical Evaluation of Seeds and Oil of Nontraditional Oil Seeds

    Directory of Open Access Journals (Sweden)

    Adam Ismail Ahmed

    2015-08-01

    Full Text Available The present work was conducted in the Laboratory of Biochemistry and Food science department, Faculty of Natural Resources and Environmental Studies, University of Kordofan, in order to evaluate some nontraditional oil seeds these are i.e. Marula (Sclerocarya birrea, Roselle (Hibiscus sabdariffa L. seeds and Christ’s thorn (Zizyphus spina-christi seeds. The seeds of the roselle and Christ’s thorn fruits were procured from Elobeid local market, North Kordofan State, while marula fruits were obtained from Elnuhod, West Kordofan State. The proximate composition of the seeds, cake and christ’s thorn pulp was done. Some chemical and physical properties were performed for the extracted oil. The results revealed that proximate composition of the seeds and cake differ statistically among the studied materials. Significant differences were observed among the oil extracted from these species; moreover, these oils differ significantly in color and viscosity only.

  5. Psühholoog Parmak: missioonide vahel peaks sõdur aasta puhkama / Merle Parmak ; intervjueerinud Kadri Ibrus

    Index Scriptorium Estoniae

    Parmak, Merle, 1968-

    2009-01-01

    Intervjuu kaitseväe ühendatud õppeasutuste rakendusuuringute keskuse psühholoogiga sõdurite psühholoogilise nõustamise praegusest olukorrast, posttraumaatilisest stressist, missioonidevahelise puhkeperioodi vajalikkusest

  6. Investigation of the pathogenesis of autoimmune diseases by iPS cells.

    Science.gov (United States)

    Natsumoto, Bunki; Shoda, Hirofumi; Fujio, Keishi; Otsu, Makoto; Yamamoto, Kazuhiko

    2017-01-01

    The pluripotent stem cells have a self-renewal ability and can be differentiated into theoretically all of cell types. The induced pluripotent stem (iPS) cells overcame the ethical problems of the human embryonic stem (ES) cell, and enable pathologic analysis of intractable diseases and drug discovery. The in vitro disease model using disease-specific iPS cells enables repeated analyses of human cells without influence of environment factors. Even though autoimmune diseases are polygenic diseases, autoimmune disease-specific iPS cells are thought to be a promising tool for analyzing the pathogenesis of the diseases and drug discovery in future.

  7. Evaluation of papaya seeds as a novel non-conventional low-cost adsorbent for removal of methylene blue.

    Science.gov (United States)

    Hameed, B H

    2009-03-15

    The feasibility of using papaya seeds (PS), abundantly available waste in Malaysia, for the cationic dye (methylene blue) adsorption has been investigated. Batch adsorption studies were conducted to study the effects of contact time, initial concentration (50-360 mg/L), pH (3-10) and adsorbent dose (0.05-1.00 g) on the removal of methylene blue (MB) at temperature of 30 degrees C. The equilibrium data were analyzed by the Langmuir, the Freundlich and the Temkin isotherms. The data fitted well with the Langmuir model with a maximum adsorption capacity of 555.557 mg/g. The pseudo-second-order kinetics was the best for the adsorption of MB by PS with good correlation. The results demonstrated that the PS is very effective to remove methylene blue from aqueous solutions.

  8. Sunflower seed allergy.

    Science.gov (United States)

    Ukleja-Sokołowska, Natalia; Gawrońska-Ukleja, Ewa; Żbikowska-Gotz, Magdalena; Bartuzi, Zbigniew; Sokołowski, Łukasz

    2016-09-01

    Sunflower seeds are a rare source of allergy, but several cases of occupational allergies to sunflowers have been described. Sunflower allergens on the whole, however, still await precise and systematic description. We present an interesting case of a 40-year-old male patient, admitted to hospital due to shortness of breath and urticaria, both of which appeared shortly after the patient ingested sunflower seeds. Our laryngological examination revealed swelling of the pharynx with retention of saliva and swelling of the mouth and tongue. During diagnostics, 2 months later, we found that skin prick tests were positive to mugwort pollen (12/9 mm), oranges (6/6 mm), egg protein (3/3 mm), and hazelnuts (3/3 mm). A native prick by prick test with sunflower seeds was strongly positive (8/5 mm). Elevated concentrations of specific IgE against weed mix (inc. lenscale, mugwort, ragweed) allergens (1.04 IU/mL), Artemisia vulgaris (1.36 IU/mL), and Artemisia absinthium (0.49 IU/mL) were found. An ImmunoCap ISAC test found an average level of specific IgE against mugwort pollen allergen component Art v 1 - 5,7 ISU-E, indicating an allergy to mugwort pollen and low to medium levels of specific IgE against lipid transfer proteins (LTP) found in walnuts, peanuts, mugwort pollen, and hazelnuts. Through the ISAC inhibition test we proved that sunflower seed allergen extracts contain proteins cross-reactive with patients' IgE specific to Art v 1, Art v 3, and Jug r 3. Based on our results and the clinical pattern of the disease we confirmed that the patient is allergic to mugwort pollen and that he had an anaphylactic reaction as a result of ingesting sunflower seeds. We suspected that hypersensitivity to sunflower LTP and defensin-like proteins, both cross-reactive with mugwort pollen allergens, were the main cause of the patient's anaphylactic reaction. © The Author(s) 2016.

  9. Targeted particle tracking in computational models of human carotid bifurcations.

    Science.gov (United States)

    Marshall, Ian

    2011-12-01

    A significant and largely unsolved problem of computational fluid dynamics (CFD) simulation of flow in anatomically relevant geometries is that very few calculated pathlines pass through regions of complex flow. This in turn limits the ability of CFD-based simulations of imaging techniques (such as MRI) to correctly predict in vivo performance. In this work, I present two methods designed to overcome this filling problem, firstly, by releasing additional particles from areas of the flow inlet that lead directly to the complex flow region ("preferential seeding") and, secondly, by tracking particles both "downstream" and "upstream" from seed points within the complex flow region itself. I use the human carotid bifurcation as an example of complex blood flow that is of great clinical interest. Both idealized and healthy volunteer geometries are investigated. With uniform seeding in the inlet plane (in the common carotid artery (CCA)) of an idealized bifurcation geometry, approximately half the particles passed through the internal carotid artery (ICA) and half through the external carotid artery. However, of those particles entering the ICA, only 16% passed directly through the carotid bulb region. Preferential seeding from selected regions of the CCA was able to increase this figure to 47%. In the second method, seeding of particles within the carotid bulb region itself led to a very high proportion (97%) of pathlines running from CCA to ICA. Seeding of particles in the bulb plane of three healthy volunteer carotid bifurcation geometries led to much better filling of the bulb regions than by particles seeded at the inlet alone. In all cases, visualization of the origin and behavior of recirculating particles led to useful insights into the complex flow patterns. Both seeding methods produced significant improvements in filling the carotid bulb region with particle tracks compared with uniform seeding at the inlet and led to an improved understanding of the complex

  10. Salinity reduced growth PS2 photochemistry and chlorophyll content in radish Crescimento reduzido por salinidade, fotoquímica PS2 e conteúdo de clorofila em rabanete

    Directory of Open Access Journals (Sweden)

    Muhammad Jamil

    2007-01-01

    Full Text Available When plants are grown under saline conditions, photosynthetic activity decreases leading to reduced plant growth, leaf area, chlorophyll content and chlorophyll fluorescence. Seeds and seedlings of radish (Raphanus sativus L. were grown in NaCl solutions under controlled greenhouse conditions. The NaCl concentrations in complete nutrient solutions were 0 (control, 4.7, 9.4 and 14.1 dS m-1. The salinity reduced germination percentage and also delayed the germination rate as the salt level increased. Lengths and fresh weights of root and shoot decreased with the increasing salt concentration. Furthermore, photochemical efficiency of PS2 (Fv/Fm, photochemical quenching coefficient (qP, non photochemical quenching coefficient (qN, leaf area and chlorophyll content (SPAD value were also reduced (P Quando plantas crescem sob condições de salinidade, sua atividade fotossintética diminui levando a um crescimento reduzido, menor área foliar, conteúdo de clorofila e fluorescência de clorofila. Sementes e plântulas de rabanete (Raphanus sativus L. foram germinadas e conduzidas em soluções de NaCl sob condições controladas de casa de vegetação. As concentrações de NaCl adicionado a solução nutritiva completa foram 0 (Controle, 4,7; 9,4 e 14,1 dS m-1. A salinidade diminui a percentagem de germinação e também atrasou a taxa de germinação com o aumento do nível de sal. Comprimento e peso fresco da parte aérea e da raiz decresceram com o aumento da concentração salina. Além disso, a eficiência fotoquímica de PS2 (Fv/Fm, o coeficiente fotoquímico de 'quenchin" (q p, o coeficiente não fotoquímico de "quenching" (q n, a área foliar e o teor de clorofila (valor SPAD também foram reduzidos (P < 0,001 por estresse de sal. Ao contrário, a relação Fo/Fm aumentou com a concentração salina, ao passo que a salinidade não teve efeito sobre a eficiência de excitação capturada pelo PS2 aberto (Fv'/Fm', taxa de transporte eletr

  11. Antibody-directed lentiviral gene transduction for live-cell monitoring and selection of human iPS and hES cells.

    Directory of Open Access Journals (Sweden)

    Dai-tze Wu

    Full Text Available The identification of stem cells within a mixed population of cells is a major hurdle for stem cell biology--in particular, in the identification of induced pluripotent stem (iPS cells during the reprogramming process. Based on the selective expression of stem cell surface markers, a method to specifically infect stem cells through antibody-conjugated lentiviral particles has been developed that can deliver both visual markers for live-cell imaging as well as selectable markers to enrich for iPS cells. Antibodies recognizing SSEA4 and CD24 mediated the selective infection of the iPS cells over the parental human fibroblasts, allowing for rapid expansion of these cells by puromycin selection. Adaptation of the vector allows for the selective marking of human embryonic stem (hES cells for their removal from a population of differentiated cells. This method has the benefit that it not only identifies stem cells, but that specific genes, including positive and negative selection markers, regulatory genes or miRNA can be delivered to the targeted stem cells. The ability to specifically target gene delivery to human pluripotent stem cells has broad applications in tissue engineering and stem cell therapies.

  12. Particle physics

    CERN Document Server

    Martin, Brian R

    2017-01-01

    An accessible and carefully structured introduction to Particle Physics, including important coverage of the Higgs Boson and recent progress in neutrino physics. Fourth edition of this successful title in the Manchester Physics series. Includes information on recent key discoveries including : An account of the discovery of exotic hadrons, beyond the simple quark model; Expanded treatments of neutrino physics and CP violation in B-decays; An updated account of ‘physics beyond the standard model’, including the interaction of particle physics with cosmology; Additional problems in all chapters, with solutions to selected problems available on the book’s website; Advanced material appears in optional starred sections.

  13. Physicochemical properties and potential food applications of Moringa oleifera seed oil blended with other vegetable oils.

    Science.gov (United States)

    Dollah, Sarafhana; Abdulkarim, Sabo Muhammad; Ahmad, Siti Hajar; Khoramnia, Anahita; Ghazali, Hasanah Mohd

    2014-01-01

    Blends (30:70, 50:50 and 70:30 w/w) of Moringa oleifera seed oil (MoO) with palm olein (PO), palm stearin (PS), palm kernel oil (PKO) and virgin coconut oil (VCO) were prepared. To determine the physicochemical properties of the blends, the iodine value (IV), saponication value (SV), fatty acid (FA) composition, triacylglycerol (TAG) composition, thermal behaviour (DSC) and solid fat content (SFC) tests were analysed. The incorporation of high oleic acid (81.73%) MoO into the blends resulted in the reduction of palmitic acid content of PO and PS from 36.38% to 17.17% and 54.66% to 14.39% and lauric acid content of PKO and VCO from 50.63% to 17.70% and 51.26% to 26.05% respectively while oleic acid and degree of unsaturation were increased in all blends. Changes in the FA composition and TAG profile have significantly affected the thermal behavior and solid fat content of the oil blends. In MoO/PO blends the melting temperature of MoO decreased while, in MoO/PS, MoO/PKO and MoO/VCO blends, it increased indicating produce of zero-trans harder oil blends without use of partial hydrogenation. The spreadability of PS, PKO and VCO in low temperatures was also increased due to incorporation of MoO. The melting point of PS significantly decreased in MoO/PS blends which proved to be suitable for high oleic bakery shortening and confectionary shortening formulation. The finding appears that blending of MoO with other vegetable oils would enable the initial properties of the oils to be modified or altered and provide functional and nutritional attributes for usage in various food applications, increasing the possibilities for the commercial use of these oils.

  14. File list: DNS.PSC.10.AllAg.iPS_derived_fibroblasts [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.PSC.10.AllAg.iPS_derived_fibroblasts hg19 DNase-seq Pluripotent stem cell iPS derived... fibroblasts http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/DNS.PSC.10.AllAg.iPS_derived_fibroblasts.bed ...

  15. File list: NoD.PSC.05.AllAg.iPS_derived_fibroblasts [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.PSC.05.AllAg.iPS_derived_fibroblasts hg19 No description Pluripotent stem cell iPS derived... fibroblasts http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/NoD.PSC.05.AllAg.iPS_derived_fibroblasts.bed ...

  16. File list: InP.PSC.10.AllAg.iPS_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.PSC.10.AllAg.iPS_derived_neural_cells hg19 Input control Pluripotent stem cell iPS derived... neural cells SRX702550 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/InP.PSC.10.AllAg.iPS_derived_neural_cells.bed ...

  17. File list: Pol.PSC.20.AllAg.iPS_derived_fibroblasts [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.20.AllAg.iPS_derived_fibroblasts hg19 RNA polymerase Pluripotent stem cell iPS derived... fibroblasts http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.PSC.20.AllAg.iPS_derived_fibroblasts.bed ...

  18. File list: NoD.PSC.50.AllAg.iPS_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.PSC.50.AllAg.iPS_derived_neural_cells hg19 No description Pluripotent stem cell iPS derived... neural cells http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/NoD.PSC.50.AllAg.iPS_derived_neural_cells.bed ...

  19. File list: DNS.PSC.05.AllAg.iPS_derived_fibroblasts [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.PSC.05.AllAg.iPS_derived_fibroblasts hg19 DNase-seq Pluripotent stem cell iPS derived... fibroblasts http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/DNS.PSC.05.AllAg.iPS_derived_fibroblasts.bed ...

  20. File list: Pol.PSC.20.AllAg.iPS_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.20.AllAg.iPS_derived_neural_cells hg19 RNA polymerase Pluripotent stem cell iPS derived... neural cells http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.PSC.20.AllAg.iPS_derived_neural_cells.bed ...

  1. File list: Pol.PSC.50.AllAg.iPS_derived_fibroblasts [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.50.AllAg.iPS_derived_fibroblasts hg19 RNA polymerase Pluripotent stem cell iPS derived... fibroblasts http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.PSC.50.AllAg.iPS_derived_fibroblasts.bed ...

  2. File list: InP.PSC.20.AllAg.iPS_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.PSC.20.AllAg.iPS_derived_neural_cells hg19 Input control Pluripotent stem cell iPS derived... neural cells SRX702550 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/InP.PSC.20.AllAg.iPS_derived_neural_cells.bed ...

  3. File list: Oth.PSC.10.AllAg.iPS_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.PSC.10.AllAg.iPS_derived_neural_cells hg19 TFs and others Pluripotent stem cell iPS derived...X968910 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.PSC.10.AllAg.iPS_derived_neural_cells.bed ...

  4. File list: ALL.PSC.10.AllAg.iPS_derived_fibroblasts [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.PSC.10.AllAg.iPS_derived_fibroblasts hg19 All antigens Pluripotent stem cell iPS derived...4,SRX1099982,SRX1099970,SRX1099965,SRX1099961,SRX1099958,SRX1099949 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/ALL.PSC.10.AllAg.iPS_derived_fibroblasts.bed ...

  5. File list: DNS.PSC.20.AllAg.iPS_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.PSC.20.AllAg.iPS_derived_neural_cells hg19 DNase-seq Pluripotent stem cell iPS derived... neural cells http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/DNS.PSC.20.AllAg.iPS_derived_neural_cells.bed ...

  6. File list: NoD.PSC.05.AllAg.iPS_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.PSC.05.AllAg.iPS_derived_neural_cells hg19 No description Pluripotent stem cell iPS derived neural... cells http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/NoD.PSC.05.AllAg.iPS_derived_neural_cells.bed ...

  7. File list: His.PSC.50.AllAg.iPS_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.PSC.50.AllAg.iPS_derived_neural_cells hg19 Histone Pluripotent stem cell iPS derived neural...archive.biosciencedbc.jp/kyushu-u/hg19/assembled/His.PSC.50.AllAg.iPS_derived_neural_cells.bed ...

  8. File list: DNS.PSC.10.AllAg.iPS_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.PSC.10.AllAg.iPS_derived_neural_cells hg19 DNase-seq Pluripotent stem cell iPS derived neural... cells http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/DNS.PSC.10.AllAg.iPS_derived_neural_cells.bed ...

  9. File list: ALL.PSC.10.AllAg.iPS_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.PSC.10.AllAg.iPS_derived_neural_cells hg19 All antigens Pluripotent stem cell iPS derived neural...hive.biosciencedbc.jp/kyushu-u/hg19/assembled/ALL.PSC.10.AllAg.iPS_derived_neural_cells.bed ...

  10. File list: Unc.PSC.10.AllAg.iPS_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Unc.PSC.10.AllAg.iPS_derived_neural_cells hg19 Unclassified Pluripotent stem cell iPS derived neural... cells http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Unc.PSC.10.AllAg.iPS_derived_neural_cells.bed ...

  11. File list: DNS.PSC.05.AllAg.iPS_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.PSC.05.AllAg.iPS_derived_neural_cells hg19 DNase-seq Pluripotent stem cell iPS derived neural... cells http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/DNS.PSC.05.AllAg.iPS_derived_neural_cells.bed ...

  12. File list: Unc.PSC.20.AllAg.iPS_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Unc.PSC.20.AllAg.iPS_derived_neural_cells hg19 Unclassified Pluripotent stem cell iPS derived neural... cells http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Unc.PSC.20.AllAg.iPS_derived_neural_cells.bed ...

  13. File list: Oth.PSC.05.AllAg.iPS_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.PSC.05.AllAg.iPS_derived_neural_cells hg19 TFs and others Pluripotent stem cell iPS derived neural...X968908 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.PSC.05.AllAg.iPS_derived_neural_cells.bed ...

  14. File list: His.PSC.20.AllAg.iPS_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.PSC.20.AllAg.iPS_derived_neural_cells hg19 Histone Pluripotent stem cell iPS derived neural...archive.biosciencedbc.jp/kyushu-u/hg19/assembled/His.PSC.20.AllAg.iPS_derived_neural_cells.bed ...

  15. File list: ALL.PSC.50.AllAg.iPS_derived_fibroblasts [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.PSC.50.AllAg.iPS_derived_fibroblasts hg19 All antigens Pluripotent stem cell iPS...8,SRX1099970,SRX1099949,SRX1099977,SRX1099965,SRX1099969,SRX1099955 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/ALL.PSC.50.AllAg.iPS_derived_fibroblasts.bed ...

  16. File list: His.PSC.10.AllAg.iPS_derived_fibroblasts [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.PSC.10.AllAg.iPS_derived_fibroblasts hg19 Histone Pluripotent stem cell iPS der...ived fibroblasts http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/His.PSC.10.AllAg.iPS_derived_fibroblasts.bed ...

  17. File list: InP.PSC.05.AllAg.iPS_derived_fibroblasts [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.PSC.05.AllAg.iPS_derived_fibroblasts hg19 Input control Pluripotent stem cell iPS... derived fibroblasts http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/InP.PSC.05.AllAg.iPS_derived_fibroblasts.bed ...

  18. File list: Unc.PSC.05.AllAg.iPS_derived_fibroblasts [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  19. File list: ALL.PSC.20.AllAg.iPS_derived_fibroblasts [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  20. File list: Pol.PSC.10.AllAg.iPS_derived_fibroblasts [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.10.AllAg.iPS_derived_fibroblasts hg19 RNA polymerase Pluripotent stem cell iPS... derived fibroblasts http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.PSC.10.AllAg.iPS_derived_fibroblasts.bed ...