Attenuated bioluminescent Brucella melitensis mutants GR019 (virB4), GR024 (galE), and GR026 (BMEI1090-BMEI1091) confer protection in mice.

Science.gov (United States)

Rajashekara, Gireesh; Glover, David A; Banai, Menachem; O'Callaghan, David; Splitter, Gary A

2006-05-01

In vivo bioluminescence imaging is a persuasive approach to investigate a number of issues in microbial pathogenesis. Previously, we have applied bioluminescence imaging to gain greater insight into Brucella melitensis pathogenesis. Endowing Brucella with bioluminescence allowed direct visualization of bacterial dissemination, pattern of tissue localization, and the contribution of Brucella genes to virulence. In this report, we describe the pathogenicity of three attenuated bioluminescent B. melitensis mutants, GR019 (virB4), GR024 (galE), and GR026 (BMEI1090-BMEI1091), and the dynamics of bioluminescent virulent bacterial infection following vaccination with these mutants. The virB4, galE, and BMEI1090-BMEI1091 mutants were attenuated in interferon regulatory factor 1-deficient (IRF-1(-/-)) mice; however, only the GR019 (virB4) mutant was attenuated in cultured macrophages. Therefore, in vivo imaging provides a comprehensive approach to identify virulence genes that are relevant to in vivo pathogenesis. Our results provide greater insights into the role of galE in virulence and also suggest that BMEI1090 and downstream genes constitute a novel set of genes involved in Brucella virulence. Survival of the vaccine strain in the host for a critical period is important for effective Brucella vaccines. The galE mutant induced no changes in liver and spleen but localized chronically in the tail and protected IRF-1(-/-) and wild-type mice from virulent challenge, implying that this mutant may serve as a potential vaccine candidate in future studies and that the direct visualization of Brucella may provide insight into selection of improved vaccine candidates.

Caracterização da imunogenicidade das proteínas recombinantes Virb9, Virb10 e fator termo instável de Elongação de Peptídeos de Anaplasma marginale em camundongos

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Ana Beatriz Canevari Castelão

2012-10-01

Full Text Available Considerando as limitações dos atuais métodos de controle contra a anaplasmose bovina, o desenvolvimento de uma vacina efetiva se faz necessário. A partir do advento da análise genômica e proteômica, novas proteínas de membrana de Anaplasma marginale foram identificadas como possíveis candidatas a componentes de uma vacina, tais como, VirB9,VirB10 e Fator Termo Instavél de Elongação de Peptídeos (EF-Tu. Embora estas proteínas ainda não estejam bem caracterizadas na membrana de A. marginale, a produção destas na forma recombinante (rVirB 9, rVirB10 e rEF-Tutem sido realizada, mas as mesmas ainda não foram exploradas em formulações vacinais. Neste trabalho, avaliou-se ouso de rVirB9, rVirB10 e rEF-Tu emulsionadas em adjuvante Montanide em camundongos. Nas condições testadas, verificou-se a indução de forte resposta imune humoral com a produção de IgG1 e IgG2a, sendo que as proporções dos níveis de produção destas subclasses indicam predomínio de IgG1. Entretanto, esplenócitos de animais, que foram injetados com rVirB9 ou rVirB10, produziram interferon-gama acima do limite de detecção do ensaio após estimulação in vitro, sinalizando assim resposta celular específica. Assim, novas avaliações serão realizadas com a finalidade de modular o perfil de resposta imune obtido em bovinos e avaliar a proteção contra A. marginale.

Pregnancy associated plasma protein-A (PAPP-A) is not a marker of the vulnerable atherosclerotic plaque

DEFF Research Database (Denmark)

Iversen, Kasper; Teisner, Ane; Dalager, Soren

2011-01-01

OBJECTIVE: To investigate if pregnancy associated plasma protein-A (PAPP-A) was present in the vulnerable plaque, and if not, to find alternative hypothesis for the release of PAPP-A. DESIGN AND METHODS: Vulnerable plaques and control tissues were examined by immunohistochemistry. Volunteers...

The effect of heparin on pregnancy associated plasma protein-A concentration in healthy, non-pregnant individuals

DEFF Research Database (Denmark)

Jespersen, Camilla H B; Vestergaard, Kirstine R.; Schou, Morten

2015-01-01

Objectives: The objective of this study was to determine the differences in pregnancy associated plasma protein-A (PAPP-A) concentrations in heparin naive and heparin treated healthy men and non-pregnant women, to find a possible difference in different age groups, and to determine the response...

Use and optimization of a dual-flowrate loading strategy to maximize throughput in protein-a affinity chromatography.

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Ghose, Sanchayita; Nagrath, Deepak; Hubbard, Brian; Brooks, Clayton; Cramer, Steven M

2004-01-01

The effect of an alternate strategy employing two different flowrates during loading was explored as a means of increasing system productivity in Protein-A chromatography. The effect of such a loading strategy was evaluated using a chromatographic model that was able to accurately predict experimental breakthrough curves for this Protein-A system. A gradient-based optimization routine is carried out to establish the optimal loading conditions (initial and final flowrates and switching time). The two-step loading strategy (using a higher flowrate during the initial stages followed by a lower flowrate) was evaluated for an Fc-fusion protein and was found to result in significant improvements in process throughput. In an extension of this optimization routine, dynamic loading capacity and productivity were simultaneously optimized using a weighted objective function, and this result was compared to that obtained with the single flowrate. Again, the dual-flowrate strategy was found to be superior.

SYM1, l’ortologo di lievito del gene umano MPV17, codifica per una proteina indotta da stress che modula lo stato bioenergetico e morfogenetico del mitocondrio

OpenAIRE

Dallabona, Cristina

2010-01-01

Mutazioni nel gene MPV17 causano l’insorgenza di una forma epatocerebrale della sindrome da deplezione del DNA mitocondriale. MPV17 codifica per una piccola proteina idrofobica localizzata nella membrana mitocondriale interna con funzione sconosciuta. Per cercare di chiarire le basi molecolari che causano la patologia, abbiamo utilizzato il lievito S. cerevisiae come organismo modello grazie alla presenza di un ortologo di MPV17: SYM1. Abbiamo dimostrato che la proteina Sym1 è essenziale per ...

Flow-mediated lipoprotein retention; Pregnancy-associated plasma protein-A inhibition in atherosclerosis and its release during myocardial ischemia

DEFF Research Database (Denmark)

Steffensen, Lasse Bach

, which states that retention of lipoproteins is the key initiating event of atherosclerosis development. Pregnancy-associated plasma protein-A (PAPP-A) regulates the insulin-like growth factor (IGF) system by proteolytic cleavage of a subset of IGF-binding proteins. Studies in mice have established PAPP...

Pregnancy Associated Plasma Protein-A in Type 2 Diabetic Patient with Peripheral Neuropathy

International Nuclear Information System (INIS)

Nosseir, N.M.

2011-01-01

Metabolic changes induced by hyperglycemia lead to dysregulation of cytokines control, subclinical inflammation together with oxidative stress associated with diabetes. The aim of this study is to correlate the role of type 2 diabetic neuropathy on serum pregnancy associated plasma protein-A,interleukin-6 and c-reactive protein .The results denoted that both pregnancy associated plasma protein-A and interleukin-6 were significantly increased in those patients with diabetic neuropathy compared with those without neuropathy but while c-reactive proteins showed significant differences between the three groups, the results lead to the conclusion that PAPP-A,IL-6 are useful tests in monitoring the neuropathic complications associated with type 2 diabetes

Pregnancy-associated plasma protein-a levels in individuals with and without coronary artery disease

International Nuclear Information System (INIS)

Khan, N.U.; Khan, F.A.; Khan, D.A.; Asim, N.

2011-01-01

Objective: To compare pregnancy-associated plasma protein-A (PAPP-A) levels in individuals with and without coronary artery disease (CAD). Study Design: Cross-sectional comparative study. Place and Duration of Study: Department of Chemical Pathology and Endocrinology, Armed Forces Institute of Pathology (AFIP), Rawalpindi, in collaboration with Armed Forces Institute of Cardiology (AFIC), from September 2008 to March 2010. Methodology: One hundred and twenty five (125) individuals both male and female were included in the study. Blood for PAPP-A and lipid profile was collected, just before angiography. On the basis of angiography, the individuals were divided into those with and without CAD. PAPP-A was analyzed by using Diagnostic System Laboratories (DSL) Enzyme Linked Immunosorbent Assay (ELISA) kit and reading was taken by ELISA reader. Lipid profile was determined on automated analyzers Selectra-2 and Vitros 5.1. Results: Amongst the 125 individuals, 41 individuals were without CAD whereas 84 individuals were having CAD. Mean PAPP-A levels were 0.74 +- 0.35 mIU/L in those without CAD whereas mean PAPP-A levels in those with CAD were 1.35 +- 0.57 mIU/L. The difference between the two groups was statistically significant (p < 0.001). A PAPP-A cut off level of 0.85 mIU/L had a sensitivity and specificity of 78% and 70% respectively for diagnosing atherosclerotic CAD. Conclusion: PAPP-A is a potentially relevant marker of the presence and extent of coronary atherosclerosis as its levels are elevated in CAD as compared to individuals without CAD. Pregnancy-associated plasma protein-A. (author)

Temporal course of pregnancy-associated plasma protein-A in angioplasty-treated ST-elevation myocardial infarction patients and potential significance of concomitant heparin administration

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Terkelsen, Christian J; Oxvig, Claus; Nørgaard, Bjarne L

2009-01-01

Pregnancy-associated plasma protein-A (PAPP-A) is a putative plaque instability marker. In acute coronary syndromes, the disrupted culprit plaque contains abundant PAPP-A, and circulating PAPP-A levels predict clinical outcomes. Determinants of circulating PAPP-A levels, however, are not fully...

Improved prediction of gestational hypertension by inclusion of placental growth factor and pregnancy associated plasma protein-a in a sample of Ghanaian women

NARCIS (Netherlands)

Antwi, Edward; Klipstein-Grobusch, Kerstin; Browne, Joyce L; Schielen, Peter C; Koram, Kwadwo A; Agyepong, Irene A; Grobbee, Diederick E

2018-01-01

BACKGROUND: We assessed whether adding the biomarkers Pregnancy Associated Plasma Protein-A (PAPP-A) and Placental Growth Factor (PlGF) to maternal clinical characteristics improved the prediction of a previously developed model for gestational hypertension in a cohort of Ghanaian pregnant women.

Longitudinal changes in C-reactive protein, proform of eosinophil major basic protein, and pregnancy-associated plasma protein-A during weight changes in obese children

DEFF Research Database (Denmark)

Lausten-Thomsen, Ulrik; Gamborg, Michael; Bøjsøe, Christine

2015-01-01

BACKGROUND: Childhood obesity is associated with several complications, including cardiovascular comorbidity. Several biomarkers, such as high-sensitive C-reactive protein (hs-CRP), proform of eosinophil major basic protein (Pro-MBP) and pregnancy associated plasma protein-A (PAPP-A), have equally...

Regulation of IGF binding protein proteolysis by pregnancy-associated plasma protein-ARegulation of IGF binding protein proteolysis by pregnancy-associated plasma protein-A

DEFF Research Database (Denmark)

Gaidamauskas, Ervinas

During his PhD studies, Ervinas Gaidamauskas researched the proteins pregnancy-associated plasma protein-A (PAPP-A) and its homologue PAPP-A2 in vitro. As suggested by its name, PAPP-A plays an important role in pregnancy and fetal development. Additionally, recent studies indicate a newly...

First trimesters Pregnancy-Associated Plasma Protein-A levels value to Predict Gestational diabetes Mellitus: A systematic review and meta-analysis of the literature.

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Talasaz, Zahra Hadizadeh; Sadeghi, Ramin; Askari, Fariba; Dadgar, Salmeh; Vatanchi, Atiyeh

2018-04-01

Detecting pregnant women at risk of diabetes in first months can help them by early intervention for delaying or preventing onset of GDM. In this study, we aimed to assess the Predictive value of first trimester Pregnancy related plasma protein-A (PAPP-A) levels for detecting Gestational diabetes Mellitus (GDM). This systematic review and meta-analysis was conducted through probing in databases. PubMed, Scopus, Medline and Google scholar citations were searched to find the published papers from 1974 to 2017. Studies were considered eligible if they were cohorts, case-control studies, reported GDM result, not other types, conducted on singleton pregnancy, measured Serum pregnancy associated plasma protein A in the first trimester and evaluated the relation of first trimester pregnancy associated plasma protein-A and GDM. Two reviewers independently assessed the quality with Newcastle-Ottawa and extracted data in the Pre-defined checklist. Analysis of the data was carried out by "Comprehensive Meta-analysis Version 2 (CAM)" and Metadisc software. 17 articles have our inclusion criteria and were considered in our systematic review, 5 studies included in Meta-analysis. Meta-analysis of these articles showed that the predictive value of PAPP-A for GDM has 55% sensitivity (53-58), 90% (89-90) specificity, LR + 2.48 (0.83-7.36) and LR - 0.70 (0.45-1.09) with 95% confidence intervals. In our study PAPP-A has low predictive accuracy overall, but it may be useful when combined with other tests, and this is an active part for future research. One limitation of our study is significant heterogeneity because of different adjusted variables and varied diagnostic criteria. Copyright © 2018. Published by Elsevier B.V.

Utilización de proteina de soya y carragenina en salchichas tipo Huacho con bajo tenor graso

OpenAIRE

Salva Ruíz, Bettit Karim

2008-01-01

Universidad Nacional Agraria La Molina. Escuela de Posgrado. Maestría en Tecnología de Alimentos El presente trabajo de investigación consistió en determinar la influencia de la sustitución de grasa por la adición de hidrocoloides (proteina texturizada de soya, carragenina, concentrado funcional de soya), pellejo de cerdo y combinaciones de estas en las características de la salchicha tipo Huacho. Se emplearon concentraciones de 20, 30, 40 y 50% de proteína texturizada de soya, para reduci...

Complete cDNA sequence of the preproform of human pregnancy-associated plasma protein-A. Evidence for expression in the brain and induction by cAMP

DEFF Research Database (Denmark)

Haaning, Jesper; Oxvig, Claus; Overgaard, Michael Toft

1996-01-01

A cDNA that encodes the prepropeptide of pregnancy-associated plasma protein-A (preproPAPP-A), a putative metalloproteinase, has been cloned and sequenced. PAPP-A is synthesized in the placenta as a 1627-residue precursor preproprotein with a putative 22-residue signal peptide and a highly basic...

First trimesters Pregnancy-Associated Plasma Protein-A levels value to Predict Gestational diabetes Mellitus: A systematic review and meta-analysis of the literature

Directory of Open Access Journals (Sweden)

Zahra Hadizadeh Talasaz

2018-04-01

Full Text Available Detecting pregnant women at risk of diabetes in first months can help them by early intervention for delaying or preventing onset of GDM. In this study, we aimed to assess the Predictive value of first trimester Pregnancy related plasma protein-A (PAPP-A levels for detecting Gestational diabetes Mellitus (GDM. This systematic review and meta-analysis was conducted through probing in databases. PubMed, Scopus, Medline and Google scholar citations were searched to find the published papers from 1974 to 2017. Studies were considered eligible if they were cohorts, case–control studies, reported GDM result, not other types, conducted on singleton pregnancy, measured Serum pregnancy associated plasma protein A in the first trimester and evaluated the relation of first trimester pregnancy associated plasma protein–A and GDM. Two reviewers independently assessed the quality with Newcastle–Ottawa and extracted data in the Pre-defined checklist. Analysis of the data was carried out by “Comprehensive Meta-analysis Version 2 (CAM” and Metadisc software. 17 articles have our inclusion criteria and were considered in our systematic review, 5 studies included in Meta-analysis. Meta-analysis of these articles showed that the predictive value of PAPP-A for GDM has 55% sensitivity (53–58, 90% (89–90 specificity, LR + 2.48 (0.83–7.36 and LR − 0.70 (0.45–1.09 with 95% confidence intervals. In our study PAPP-A has low predictive accuracy overall, but it may be useful when combined with other tests, and this is an active part for future research. One limitation of our study is significant heterogeneity because of different adjusted variables and varied diagnostic criteria. Keywords: Gestational diabetes Mellitus, Pregnancy-Associated Plasma Protein-A, First trimesters, Systematic review

Pregnancy associated plasma protein-A as a marker for myocardial infarction and death in patients with stable coronary artery disease: A prognostic study within the CLARICOR Trial

DEFF Research Database (Denmark)

Iversen, Kasper K; Teisner, Børge; Winkel, Per

2011-01-01

OBJECTIVE: Pregnancy associated plasma protein-A (PAPP-A) is a potential new marker for vulnerable plaques in the coronary arteries only examined in stable coronary disease (CAD) in patients undergoing coronary angiography. Here we address the prognostic value of serum PAPP-A in unselected stable...

Pregnancy-associated plasma protein-A (PAPP-A) and the proform of the eosinophil major basic protein (ProMBP) are associated with increased risk of death in heart failure patients

DEFF Research Database (Denmark)

Dembic, Maja; Hedley, Paula L.; Torp-Pedersen, Christian

2017-01-01

Risk stratification and patient management in heart failure (HF) is difficult due to the unpredictable progression of the disease, necessitating the development of reliable diagnostic biomarkers to facilitate decision-making in clinical practice. Pregnancy-associated plasma protein-A (PAPP...

Wolbachia lipoproteins: abundance, localisation and serology of Wolbachia peptidoglycan associated lipoprotein and the Type IV Secretion System component, VirB6 from Brugia malayi and Aedes albopictus.

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Voronin, Denis; Guimarães, Ana F; Molyneux, Gemma R; Johnston, Kelly L; Ford, Louise; Taylor, Mark J

2014-10-06

Lipoproteins are the major agonists of Wolbachia-dependent inflammatory pathogenesis in filariasis and a validated target for drug discovery. Here we characterise the abundance, localisation and serology of the Wolbachia lipoproteins: Wolbachia peptidoglycan associated lipoprotein and the Type IV Secretion System component, VirB6. We used proteomics to confirm lipoprotein presence and relative abundance; fractionation, immunoblotting and confocal and electron immuno-microscopy for localisation and ELISA for serological analysis. Proteomic analysis of Brugia malayi adult female protein extracts confirmed the presence of two lipoproteins, previously predicted through bioinformatics: Wolbachia peptidoglycan associated lipoprotein (wBmPAL) and the Type IV Secretion System component, VirB6 (wBmVirB6). wBmPAL was among the most abundant Wolbachia proteins present in an extract of adult female worms with wBmVirB6 only detected at a much lower abundance. This differential abundance was reflected in the immunogold-labelling, which showed wBmPAL localised at numerous sites within the bacterial membranes, whereas wBmVirB6 was present as a single cluster on each bacterial cell and also located within the bacterial membranes. Immunoblotting of fractionated extracts confirmed the localisation of wBmPAL to membranes and its absence from cytosolic fractions of C6/36 mosquito cells infected with wAlbB. In whole worm mounts, antibody labelling of both lipoproteins were associated with Wolbachia. Serological analysis showed that both proteins were immunogenic and raised antibody responses in the majority of individuals infected with Wuchereria bancrofti. Two Wolbachia lipoproteins, wBmPAL and wBmVirB6, are present in extracts of Brugia malayi with wBmPAL among the most abundant of Wolbachia proteins. Both lipoproteins localised to bacterial membranes with wBmVirB6 present as a single cluster suggesting a single Type IV Secretory System on each Wolbachia cell.

Pregnancy-associated plasma protein-A in patients on Maintenance Hemodialysis

International Nuclear Information System (INIS)

Abdel-Messeih, Ph.L.

2012-01-01

A high level of serum pregnancy associated plasma protein-A (PAPP-A) has been observed in patients suffering from renal impairment. The aim of the present study is to evaluate the level of PAPP-A and to elucidate its relationship with renal osteodystrophy and renal functions in patients maintained on hemodialysis (HD). Intact parathyroid hormone (i-PTH), calcium (Ca) and phosphorus (P) levels and alkaline phosphatase activity (ALP) were measured in the serum as markers of renal osteodystrophy while the level of blood urea and serum creatinine were evaluated as markers of renal functions. The results obtained showed that for patients maintained on HD, the levels of PAPP-A, i-PTH, P, urea and creatinine, were significantly higher than controls. Significant positive correlations were obtained between PAPP-A and each of i-PTH, ALP and creatinine in the same group. After dialysis session, the level of PAPP-A increased significantly, compared to its pre -dialysis level. According to the results obtained in the current study, it could be concluded that the increase in PAPP-A level in the serum of patients maintained on hemodialysis is probably the result of chronic inflammation and impairment of kidney functions rather than renal osteodystrophy

Stanniocalcin-1 Potently Inhibits the Proteolytic Activity of the Metalloproteinase Pregnancy-associated Plasma Protein-A

DEFF Research Database (Denmark)

Kløverpris, Søren; Mikkelsen, Jakob Hauge; Pedersen, Josefine Hvidkjær

2015-01-01

regulation in these species. Several physiological functions of STC1 have been reported, although many molecular details are still lacking. We here demonstrate that STC1 is an inhibitor of the metzincin metalloproteinase, pregnancy-associated plasma protein-A (PAPP-A), which modulates insulin-like growth...... that the homologous STC2 inhibits PAPP-A proteolytic activity, and that this depends on the formation of a covalent complex between the inhibitor and the proteinase, mediated by Cys-120 of STC2. We find that STC1 is unable to bind covalently to PAPP-A, in agreement with the absence of a corresponding cysteine residue....... It rather binds to PAPP-A with high affinity (KD = 75 pm). We further demonstrate that both STC1 and STC2 show inhibitory activity toward PAPP-A2, but not selected serine proteinases and metalloproteinases. We therefore conclude that the STCs are proteinase inhibitors, probably restricted in specificity...

The proteolytic activity of pregnancy-associated plasma protein-A is potentially regulated by stanniocalcin-1 and -2 during human ovarian follicle development

DEFF Research Database (Denmark)

Jepsen, Malene R.; Kløverpris, Søren; Bøtkjær, Jane A.

2016-01-01

STUDY QUESTION: Is the proteolytic activity of pregnancy-associated plasma protein-A (PAPP-A) regulated by the stanniocalcins (STC1 and STC2) during human follicle maturation? SUMMARY ANSWER: The STCs and PAPP-A show similar expression by immunohistochemistry in developing follicles, and regulation...... of PAPP-A proteolytic activity is suggested by the identification of inhibited protein complexes between PAPP-A and STC1 or STC2 in human follicular fluid (FF). WHAT IS KNOWN ALREADY: The insulin-like growth factor (IGF)-regulating proteinase PAPP-A is secreted by the granulosa cells of estrogen...

Expression of recombinant murine pregnancy-associated plasma protein-A (PAPP-A) and a novel variant (PAPP-Ai) with differential proteolytic activity

DEFF Research Database (Denmark)

Søe, Rikke; Overgaard, Michael Toft; Thomsen, Anni R

2002-01-01

Murine pregnancy-associated plasma protein-A (PAPP-A) cDNA encoding a 1545 amino-acid protein has been cloned. We have also identified and cloned cDNA that encodes a novel variant of PAPP-A, PAPP-Ai, carrying a 29-residue highly basic insert. The point of insertion corresponds to a junction between...... with the proform of eosinophil major basic protein (proMBP). ProMBP functions as a proteinase inhibitor in the PAPP-A-proMBP complex, but whether any mechanistic parallel on regulation of proteolytic activity can be drawn between the insert of PAPP-Ai and the linkage to proMBP is not known. Importantly, these data...

Expression of cagA, virB/D Complex and/or vacA Genes in Helicobacter pylori Strains Originating from Patients with Gastric Diseases.

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Andrzej Szkaradkiewicz

Full Text Available In order to better understand pathogenicity of Helicobacter pylori, particularly in the context of its carcinogenic activity, we analysed expression of virulence genes: cagA, virB/D complex (virB4, virB7, virB8, virB9, virB10, virB11, virD4 and vacA in strains of the pathogen originating from persons with gastric diseases. The studies were conducted on 42 strains of H. pylori isolated from patients with histological diagnosis of non-atrophic gastritis-NAG (group 1, including subgroup 1 containing cagA+ isolates and subgroup 2 containing cagA- strains, multifocal atrophic gastritis-MAG (group 2 and gastric adenocarcinoma-GC (group 3. Expression of H. pylori genes was studied using microarray technology. In group 1, in all strains of H. pylori cagA+ (subgroup 1 high expression of the gene as well as of virB/D was disclosed, accompanied by moderate expression of vacA. In strains of subgroup 2 a moderate expression of vacA was detected. All strains in groups 2 and 3 carried cagA gene but they differed in its expression: a high expression was detected in isolates of group 2 and its hyperexpression in strains of group 3 (hypervirulent strains. In both groups high expression of virB/D and vacA was disclosed. Our results indicate that chronic active gastritis may be induced by both cagA+ strains of H. pylori, manifesting high expression of virB/D complex but moderate activity of vacA, and cagA- strains with moderate expression of vacA gene. On the other hand, in progression of gastric pathology and carcinogenesis linked to H. pylori a significant role was played by hypervirulent strains, manifesting a very high expression of cagA and high activity of virB/D and vacA genes.

Aplicación de la endoscopia sistemática alfanumérica codificada más cromoendoscopia para la detección de lesiones precancerosas gástricas y cáncer gástrico temprano en sujetos con riesgo promedio de cáncer gástrico

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A. Pérez-Mendoza

2018-04-01

Full Text Available Resumen: Introducción y objetivos: El cáncer gástrico es una de las principales causas de cáncer a nivel mundial. No existe una estrategia global de tamizaje. La endoscopia se ha convertido en el método de elección para tamizaje, sin embargo, no existe un método estandarizado. El uso de la endoscopia sistemática alfanumérica codificada puede aumentar la detección de lesiones gástricas. El objetivo es comparar la utilidad del sistema de endoscopia alfanumérica codificada con la endoscopia convencional para la detección de lesiones premalignas y cáncer gástrico temprano en sujetos con riesgo promedio de cáncer gástrico. Material y métodos: Estudio de corte transversal, comparativo, prospectivo y aleatorizado, en pacientes con riesgo promedio para cáncer gástrico (40-50 años, sin historia de infección por H. pylori, metaplasia intestinal, atrofia gástrica ni cirugía gastrointestinal. Antes de la endoscopia recibieron preparación gástrica (200 mg de acetilcisteína oral o 50 mg de dimeticona oral. Se realizó cromoendoscopia convencional con índigo carmín para realzar el contraste. Resultados: Fueron 50 casos consecutivos (edad promedio de 44.4 ± 3.34 años, el 60% mujeres 60%, con IMC de 27.6 ± 5.82 kg/cm2. La calidad de la imagen endoscópica fue satisfactoria en todos los casos, sin diferencias entre métodos (p = 0.817. El índice de detección de lesiones premalignas y de cáncer gástrico temprano fue del 14% (6 metaplasias intestinales y un adenocarcinoma gástrico. La Sn, Sp, VPP, VPN y la exactitud diagnóstica fueron del 100, 95, 80, 100 y 96%, respectivamente para la endoscopia sistemática alfanumérica codificada y para la endoscopia convencional 100, 45, 20, 100 y 52%, respectivamente. La endoscopia sistemática alfanumérica codificada fue mejor que la endoscopia convencional para la detección de lesiones (p = 0.003; RM = 12. Conclusión: Ambas técnicas fueron efectivas; sin embargo, el

Myocardial and Peripheral Ischemia Causes an Increase in Circulating Pregnancy-Associated Plasma Protein-A in Non-atherosclerotic, Non-heparinized Pigs.

Science.gov (United States)

Steffensen, Lasse Bach; Poulsen, Christian Bo; Shim, Jeong; Bek, Marie; Jacobsen, Kevin; Conover, Cheryl A; Bentzon, Jacob Fog; Oxvig, Claus

2015-12-01

The usefulness of circulating pregnancy-associated plasma protein-A (PAPP-A) as a biomarker for acute coronary syndrome (ACS) is widely debated. We used the pig as a model to assess PAPP-A dynamics in the setting of myocardial ischemia. Induction of myocardial ischemia by ligation of the left anterior descending (LAD) coronary artery caused a systemic rise in PAPP-A. However, the ischemic myocardium was excluded as the source of PAPP-A. Interestingly, induction of ischemia in peripheral tissues by ligation of the left femoral artery caused a systemic rise in PAPP-A originating from the left hind limb. This is the first study to demonstrate PAPP-A elevations in the absence of atherosclerosis or heparin during myocardial ischemia. Our findings thus add to the current discussion of the usefulness of PAPP-A as a biomarker for ACS.

Structure of the type IV secretion system in different strains of Anaplasma phagocytophilum

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Al-Khedery Basima

2012-11-01

Full Text Available Abstract Background Anaplasma phagocytophilum is an intracellular organism in the Order Rickettsiales that infects diverse animal species and is causing an emerging disease in humans, dogs and horses. Different strains have very different cell tropisms and virulence. For example, in the U.S., strains have been described that infect ruminants but not dogs or rodents. An intriguing question is how the strains of A. phagocytophilum differ and what different genome loci are involved in cell tropisms and/or virulence. Type IV secretion systems (T4SS are responsible for translocation of substrates across the cell membrane by mechanisms that require contact with the recipient cell. They are especially important in organisms such as the Rickettsiales which require T4SS to aid colonization and survival within both mammalian and tick vector cells. We determined the structure of the T4SS in 7 strains from the U.S. and Europe and revised the sequence of the repetitive virB6 locus of the human HZ strain. Results Although in all strains the T4SS conforms to the previously described split loci for vir genes, there is great diversity within these loci among strains. This is particularly evident in the virB2 and virB6 which are postulated to encode the secretion channel and proteins exposed on the bacterial surface. VirB6-4 has an unusual highly repetitive structure and can have a molecular weight greater than 500,000. For many of the virs, phylogenetic trees position A. phagocytophilum strains infecting ruminants in the U.S. and Europe distant from strains infecting humans and dogs in the U.S. Conclusions Our study reveals evidence of gene duplication and considerable diversity of T4SS components in strains infecting different animals. The diversity in virB2 is in both the total number of copies, which varied from 8 to 15 in the herein characterized strains, and in the sequence of each copy. The diversity in virB6 is in the sequence of each of the 4 copies in

Motor and memory testing of long-lived pregnancy-associated plasma protein--a knock-out mice.

Science.gov (United States)

Mason, Emily J; Grell, Jacquelyn A; West, Sally A; Conover, Cheryl A

2014-12-01

Mice deficient in pregnancy-associated plasma protein-A (PAPP-A), an IGF binding protein protease, have been shown to be resistant to experimentally induced atherosclerosis and diabetic nephropathy, and, in the laboratory environment, live 30-40% longer than wild-type littermates in association with delayed incidence and occurrence of age-related neoplasms and degenerative diseases. PAPP-A is highly expressed in the cerebellum and hippocampus of the mouse brain. Therefore, the studies presented here were aimed at determining motor behavior, learning and retention in PAPP-A knock-out (KO) mice compared to wild-type (WT) littermates with age. Balance and coordination were assessed using an accelerating rotarod; learning and memory were assessed in a Stone T-maze. Time on the rotarod decreased with age but there was no significant difference between PAPP-A KO and WT mice at any of the testing ages. Latency to reach the goal box and number of errors committed in the Stone T-maze did not change with age and there were no significant differences between PAPP-A KO and WT mice. Lack of PAPP-A in mice did not impact central regulation of coordination, learning or memory. Copyright © 2014 Elsevier Ltd. All rights reserved.

Purification of barley dimeric α-amylase inhibitor-1 (BDAI-1) and avenin-like protein-a (ALP) from beer and their impact on beer foam stability.

Science.gov (United States)

Iimure, Takashi; Kihara, Makoto; Sato, Kazuhiro; Ogushi, Kensuke

2015-04-01

Foam stability is a key factor of beer quality for consumers and brewers. Recent beer proteome analyses have suggested that barley dimeric α-amylase inhibitor-1 (BDAI-1) and avenin-like protein-a (ALP) derived from barley are important for beer foam stability. In this study, BDAI-1 and ALP were purified from a Japanese commercial beer sample using salt precipitation and column chromatography. The purification level was verified using two-dimensional gel electrophoresis, mass spectrometry, and database searches. Purified BDAI-1 and ALP were added to a beer sample to compare the foam stability to that of a control beer sample. As a result, beer foam stability was significantly improved by BDAI-1 but not by ALP, thereby suggesting that BDAI-1 affects beer foam stability whereas ALP does not. Copyright © 2014 Elsevier Ltd. All rights reserved.

Evaluation of Serum Levels of Pregnancy Associated Plasma Protein-A, Tumor Necrosis Factor-alpha and Highly Sensitive C-Reactive Protein in Diabetic Children

International Nuclear Information System (INIS)

Abdel-Messeih, PH.L.; El-safie, A.I.; Said, A.I.

2011-01-01

Recent evidence favours the primary role of cellular auto immunity and its humoral mediators in the pathogenesis and follow up of children with type 1 diabetes mellitus (type 1 DM). The present study is carried out to investigate serum levels of pregnancy associated plasma protein-A (PAPP-A), tumor necrosis factor-alpha (TNF-alpha ) and highly sensitive C-reactive protein (hs-CRP) in children with type 1 DM. Potential role of body mass index (BMI) was evaluated. Circulating levels of TNF-alpha, PAPP-A and hs-CRP are significantly increased in children with type 1 DM as compared with healthy subjects suggesting activation of inflammatory immune response system. A significant negative correlation was obtained between TNF-alpha and BMI in diabetic patients. This is highly suggestive of the availability of these non invasive indices to help further examining type 1 DM pathophysiology and monitoring pharmacological interventions to interfere with disease development and progression.

Levels of pregnancy-associated plasma protein-A in patients with coronary heart diseases and clinic significance

International Nuclear Information System (INIS)

Wang Lingyan; Cai Gaojun; Zhang Wenwei; Wang Wenzhi; Sun Wenwei; Yan Weiqun

2006-01-01

Objective: To explore the relationship between pregnancy-associated plasma protein-A (PAPP-A) and occurance, development of cardiovascular diseases, and lipids. Methods: 75 patients with coronary disease were divided into acute myocardial infarction (n=32), unstable angina pectoris (n=22) and stable angina pectoris (n=21) groups, and 60 subjects without coronary diseases were used as controls. The serum PAPP-A, IL-6, IL-10, lipids were measured in all patients and controls by different methods of enzymatically amplified two-step sandwith- type immunoassay, double antibody radio-immunoassay, ABC-HRP, auto biochemistic analytist. Results: (1) The level of PAPP-A in acute coronary syndrome (ACS, including acute myocardial infarction and unstable angina pectoris) patients was significantly higher than that in stable angina pectoris patients and controls (P<0.05). (2) There were significantly associations between PAPP-A and serum totle cholesterol, ApoA1/ApoB (r=0.348, 0.420, P<0.05). (3) The levels of IL-6 and IL-10 in coronary heart disease patients were significantly higher than those in controls (P<0.05), and the variations among acute myocardial infarction, unstable angina pectoris, stable angina pectoris patients were significantly (P<0.05). There were significantly associations between PAPP-A, IL-6 and IL-10 (Spearman r 0.446, 0.523, P<0.05). Conclusion: PAPP-A is significantly associated with occurance and development of coronary heart disease, probablely as a marker of unstable plaque in coronary heart disease. (authors)

Type IV secretion system of Brucella spp. and its effectors.

Science.gov (United States)

Ke, Yuehua; Wang, Yufei; Li, Wengfeng; Chen, Zeliang

2015-01-01

Brucella spp. are intracellular bacterial pathogens that cause infection in domestic and wild animals. They are often used as model organisms to study intracellular bacterial infections. Brucella VirB T4SS is a key virulence factor that plays important roles in mediating intracellular survival and manipulating host immune response to infection. In this review, we discuss the roles of Brucella VirB T4SS and 15 effectors that are proposed to be crucial for Brucella pathogenesis. VirB T4SS regulates the inflammation response and manipulates vesicle trafficking inside host cells. VirB T4SS also plays crucial roles in the inhibition of the host immune response and intracellular survival during infection. Here, we list the key molecular events in the intracellular life cycle of Brucella that are potentially targeted by the VirB T4SS effectors. Elucidating the functions of these effectors will help clarify the molecular role of T4SS during infection. Furthermore, studying the effectors secreted by Brucella spp. might provide insights into the mechanisms used by the bacteria to hijack the host signaling pathways and aid in the development of better vaccines and therapies against brucellosis.

ESTUDIO DE LA PARTICIPACION DE PROTEINAS CODIFICADAS EN LOS GRUPOS GENETICOS DE DOXDA Y TETH EN LA OXIDACION DEL TIOSULFATO EN ACIDITHIOBACILLUS FERROOXIDANS ATCC 23270

OpenAIRE

BEARD BORQUEZ, SIMON RODRIGO; BEARD BORQUEZ, SIMON RODRIGO

2010-01-01

El azufre es un macronutriente esencial para todos los seres vivos que forma parte de aminoácidos, cofactores y vitaminas. Algunos microorganismos son capaces de obtener su energía de la oxidación disasimilatoria del sulfuro, azufre elemental o tiosulfato, entre otros compuestos inorgánicos de azufre reducidos. En bacterias Gram-negativas se han descrito tres vías para la oxidación de compuestos de azufre: la vía del Paracoccus (PSO), la vía PSO ramificada y la vía del intermediar...

Surfactant protein-A suppresses eosinophil-mediated killing of Mycoplasma pneumoniae in allergic lungs.

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Julie G Ledford

Full Text Available Surfactant protein-A (SP-A has well-established functions in reducing bacterial and viral infections but its role in chronic lung diseases such as asthma is unclear. Mycoplasma pneumoniae (Mp frequently colonizes the airways of chronic asthmatics and is thought to contribute to exacerbations of asthma. Our lab has previously reported that during Mp infection of non-allergic airways, SP-A aides in maintaining airway homeostasis by inhibiting an overzealous TNF-alpha mediated response and, in allergic mice, SP-A regulates eosinophilic infiltration and inflammation of the airway. In the current study, we used an in vivo model with wild type (WT and SP-A(-/- allergic mice challenged with the model antigen ovalbumin (Ova that were concurrently infected with Mp (Ova+Mp to test the hypothesis that SP-A ameliorates Mp-induced stimulation of eosinophils. Thus, SP-A could protect allergic airways from injury due to release of eosinophil inflammatory products. SP-A deficient mice exhibit significant increases in inflammatory cells, mucus production and lung damage during concurrent allergic airway disease and infection (Ova+Mp as compared to the WT mice of the same treatment group. In contrast, SP-A deficient mice have significantly decreased Mp burden compared to WT mice. The eosinophil specific factor, eosinophil peroxidase (EPO, which has been implicated in pathogen killing and also in epithelial dysfunction due to oxidative damage of resident lung proteins, is enhanced in samples from allergic/infected SP-A(-/- mice as compared to WT mice. In vitro experiments using purified eosinophils and human SP-A suggest that SP-A limits the release of EPO from Mp-stimulated eosinophils thereby reducing their killing capacity. These findings are the first to demonstrate that although SP-A interferes with eosinophil-mediated biologic clearance of Mp by mediating the interaction of Mp with eosinophils, SP-A simultaneously benefits the airway by limiting inflammation

Pregnancy-associated plasma protein-A modulates the anabolic effects of parathyroid hormone in mouse bone.

Science.gov (United States)

Clifton, Kari B; Conover, Cheryl A

2015-12-01

Intermittent parathyroid hormone (PTH) is a potent anabolic therapy for bone, and several studies have implicated local insulin-like growth factor (IGF) signaling in mediating this effect. The IGF system is complex and includes ligands and receptors, as well as IGF binding proteins (IGFBPs) and IGFBP proteases. Pregnancy-associated plasma protein-A (PAPP-A) is a metalloprotease expressed by osteoblasts in vitro that has been shown to enhance local IGF action through cleavage of inhibitory IGFBP-4. This study was set up to test two specific hypotheses: 1) Intermittent PTH treatment increases the expression of IGF-I, IGFBP-4 and PAPP-A in bone in vivo, thereby increasing local IGF activity. 2) In the absence of PAPP-A, local IGF activity and the anabolic effects of PTH on bone are reduced. Wild-type (WT) and PAPP-A knock-out (KO) mice were treated with 80 μg/kg human PTH 1-34 or vehicle by subcutaneous injection five days per week for six weeks. IGF-I, IGFBP-4 and PAPP-A mRNA expression in bone were significantly increased in response to PTH treatment. PTH treatment of WT mice, but not PAPP-A KO mice, significantly increased expression of an IGF-responsive gene. Bone mineral density (BMD), as measured by DEXA, was significantly decreased in femurs of PAPP-A KO compared to WT mice with PTH treatment. Volumetric BMD, as measured by pQCT, was significantly decreased in femoral midshaft (primarily cortical bone), but not metaphysis (primarily trabecular bone), of PAPP-A KO compared to WT mice with PTH treatment. These data suggest that stimulation of PAPP-A expression by intermittent PTH treatment contributes to PTH bone anabolism in mice. Copyright © 2015 Elsevier Inc. All rights reserved.

Increase in serum pregnancy-associated plasma protein-A is correlated with increase in cardiovascular risk factors in adult patients with growth hormone deficiency.

Science.gov (United States)

Li, Linman; Ren, Wei; Li, Jinchao; Liu, Jingjing; Wang, Lingli; Zheng, Xiaoya; Liu, Dezhen; Li, Sufang; Souvenir, Rhonda; Tang, Jiping

2012-10-01

Adult Growth Hormone Deficiency (AGHD) is correlated to many adverse effects on metabolism and increased cardiovascular risk. Pregnancy-associated plasma protein-A (PAPP-A) is a protease that promotes IGF-I availability in vascular tissues in recent study, and PAPP-A levels have been proposed as an early predictor of cardiac events. The aim of our study was to compare PAPP-A levels in AGHD patients with that of healthy adult subjects to determine if there is a relationship between serum PAPP-A and glucose and lipid metabolism. Twenty AGHD patients and 20 healthy, age-matched and weight-matched persons were chosen for the study. Their weight, height, blood pressure, body mass index (BMI), body fat percentage, waist and hip circumference, and waist-hips ratio were assessed. An oral glucose tolerance test was performed and venous blood was collected from the each patient's cubital vein for biochemical analysis. Serum PAPP-A level in AGHD patients was significantly higher than that of the control group [(7.62 ± 1.62 vs. 6.54 ± 1.31) p < 0.05], and PAPP-A was positively correlated to age, BMI, waist circumference and so on. After adjusting for the waist circumference, waist-hip ratio, 2 h postprandial blood glucose, triglycerides, the serum PAPP-A in AGHD patients was positively correlated to the BMI (r = 0.728, p < 0.05) and fasting insulin (r = 0.433, p < 0.05). In a multiple step-wise regression analysis, BMI, 2 h postprandial glucose, fasting insulin, HOMA-IR were independently associated with serum PAPP-A in AGHD patients. The increase in serum PAPP-A levels is associated with abnormal glucose metabolism and increased risk of atherosclerosis in AGHD patients.

The expression of Helicobacter pylori tfs plasticity zone cluster is regulated by pH and adherence, and its composition is associated with differential gastric IL-8 secretion.

Science.gov (United States)

Silva, Bruno; Nunes, Alexandra; Vale, Filipa F; Rocha, Raquel; Gomes, João Paulo; Dias, Ricardo; Oleastro, Mónica

2017-08-01

Helicobacter pylori virulence is associated with different clinical outcomes. The existence of an intact dupA gene from tfs4b cluster has been suggested as a predictor for duodenal ulcer development. However, the role of tfs plasticity zone clusters in the development of ulcers remains unclear. We studied several H. pylori strains to characterize the gene arrangement of tfs3 and tfs4 clusters and their impact in the inflammatory response by infected gastric cells. The genome of 14 H. pylori strains isolated from Western patients, pediatric (n=10) and adult (n=4), was fully sequenced using the Illumina platform MiSeq, in addition to eight pediatric strains previously sequenced. These strains were used to infect human gastric cells, and the secreted interleukin-8 (IL-8) was quantified by ELISA. The expression of virB2, dupA, virB8, virB10, and virB6 was assessed by quantitative PCR in adherent and nonadherent fractions of H. pylori during in vitro co-infection, at different pH values. We have found that cagA-positive H. pylori strains harboring a complete tfs plasticity zone cluster significantly induce increased production of IL-8 from gastric cells. We have also found that the region spanning from virB2 to virB10 genes constitutes an operon, whose expression is increased in the adherent fraction of bacteria during infection, as well as in both adherent and nonadherent fractions at acidic conditions. A complete tfs plasticity zone cluster is a virulence factor that may be important for the colonization of H. pylori and to the development of severe outcomes of the infection with cagA-positive strains. © 2017 John Wiley & Sons Ltd.

Inducible knockdown of pregnancy-associated plasma protein-A gene expression in adult female mice extends life span.

Science.gov (United States)

Bale, Laurie K; West, Sally A; Conover, Cheryl A

2017-08-01

Pregnancy-associated plasma protein-A (PAPP-A) knockout (KO) mice, generated through homologous recombination in embryonic stem cells, have a significantly increased lifespan compared to wild-type littermates. However, it is unknown whether this longevity advantage would pertain to PAPP-A gene deletion in adult animals. In the present study, we used tamoxifen (Tam)-inducible Cre recombinase-mediated excision of the floxed PAPP-A (fPAPP-A) gene in mice at 5 months of age. fPAPP-A mice, which were either positive (pos) or negative (neg) for Tam-Cre, received Tam treatment with quarterly boosters. Only female mice could be used with this experimental design. fPAPP-A/neg and fPAPP-A/pos mice had similar weights at the start of the experiment and showed equivalent weight gain. We found that fPAPP-A/pos mice had a significant extension of life span (P = 0.005). The median life span was increased by 21% for fPAPP-A/pos compared to fPAPP-A/neg mice. Analysis of mortality in life span quartiles indicated that the proportion of deaths of fPAPP-A/pos mice were lower than fPAPP-A/neg mice at young adult ages (P = 0.002 for 601-800 days) and higher than fPAPP-A/neg mice at older ages (P = 0.004 for >1000 days). Thus, survival curves and age-specific mortality indicate that female mice with knockdown of PAPP-A gene expression as adults have an extended healthy life span. © 2017 The Authors. Aging Cell published by the Anatomical Society and John Wiley & Sons Ltd.

Detection of virulence-associated genes in Brucella melitensis ...

African Journals Online (AJOL)

The current study involved detection of three virulence genes (bvfA, virB, ure) by PCR in 52 isolates of Brucella melitensis biovar 3, recovered from different animal species (28 sheep, 10 goats, 9 cattle and 5 buffaloes). Of the 52 B. melitensis strains; 48 (92.3%) isolates carried bvfA genes, 51 (98.1%) isolates had virB genes ...

Type IV Secretion System of Brucella spp. and its Effectors

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Yuehua eKe

2015-10-01

Full Text Available Brucella spp. cause brucellosis in domestic and wild animals. They are intracellular bacterial pathogens and used as model organisms to study intracellular bacterial infections. Brucella VirB T4SS is a key virulence factor that plays important roles in mediating intracellular survival and manipulating host immune response to infection. In this review, we will discuss roles of Brucella VirB T4SS and in more detail of all 15 identified effectors, which may be crucial for Brucella pathogenesis. VirB T4SS regulates the inflammation response and manipulates vesicle trafficking inside host cells, suggesting that it plays crucial roles in the inhibition of the host immune response and intracellular survival during infection. So, we listed some key molecular events in the intracellular life cycle of Brucella potentially targeted by the VirB T4SS effectors. Elucidating functions of the effectors secreted will be crucial to clarifying mechanism of T4SS during infection. Studying the effectors secreted by Brucella spp. might provide insights into the mechanisms by which the bacteria hijack the host signaling pathways, which help us to develop better vaccines and therapies against brucellosis.

The impact of surfactant protein-A on ozone-induced changes in the mouse bronchoalveolar lavage proteome

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Floros Joanna

2009-03-01

Full Text Available Abstract Background Ozone is a major component of air pollution. Exposure to this powerful oxidizing agent can cause or exacerbate many lung conditions, especially those involving innate immunity. Surfactant protein-A (SP-A plays many roles in innate immunity by participating directly in host defense as it exerts opsonin function, or indirectly via its ability to regulate alveolar macrophages and other innate immune cells. The mechanism(s responsible for ozone-induced pathophysiology, while likely related to oxidative stress, are not well understood. Methods We employed 2-dimensional difference gel electrophoresis (2D-DIGE, a discovery proteomics approach, coupled with MALDI-ToF/ToF to compare the bronchoalveolar lavage (BAL proteomes in wild type (WT and SP-A knockout (KO mice and to assess the impact of ozone or filtered air on the expression of BAL proteins. Using the PANTHER database and the published literature most identified proteins were placed into three functional groups. Results We identified 66 proteins and focused our analysis on these proteins. Many of them fell into three categories: defense and immunity; redox regulation; and protein metabolism, modification and chaperones. In response to the oxidative stress of acute ozone exposure (2 ppm; 3 hours there were many significant changes in levels of expression of proteins in these groups. Most of the proteins in the redox group were decreased, the proteins involved in protein metabolism increased, and roughly equal numbers of increases and decreases were seen in the defense and immunity group. Responses between WT and KO mice were similar in many respects. However, the percent change was consistently greater in the KO mice and there were more changes that achieved statistical significance in the KO mice, with levels of expression in filtered air-exposed KO mice being closer to ozone-exposed WT mice than to filtered air-exposed WT mice. Conclusion We postulate that SP-A plays a role

Molecular prevalence of putative virulence-associated genes in Brucella melitensis and Brucella abortus isolates from human and livestock specimens in Iran.

Science.gov (United States)

Hashemifar, Iman; Yadegar, Abbas; Jazi, Faramarz Masjedian; Amirmozafari, Nour

2017-04-01

Molecular prevalence of nine putative virulence factors in two more prevalent Brucella species in Iranian patients and livestock was investigated. During five years (2010-2015), 120 human and animal specimens were collected from three geographical areas of Iran. All samples were cultured in blood culture media and subcultured into Brucella agar medium. Nine primer pairs were designed for detection of VirB2, VirB5, VceC, BtpA, BtpB, PrpA, BetB, BPE275 and BSPB virulence factors using PCR and sequence analysis. Totally, 68 Brucella isolates including 60 B. melitensis and 8 B. abortus were isolated from the human and animal specimens examined. Approximately, all B. melitensis and B. abortus strains were positive (100%) regarding btpA, btpB, virB5, vceC, bpe275, bspB, and virB2 genes except for prpA and betB that were detected in 86% and 97% of the strains, respectively. Significant relationships were found between the presence of prpA and human B. melitensis isolates (P = 0.04), and also between the presence of betB and human isolates of B. abortus (P = 0.03). In conclusion, our results revealed that Iranian Brucella strains, regardless of human or animal sources, are extremely virulent due to high prevalence of virulence attributes in almost all strains studied. Copyright © 2017 Elsevier Ltd. All rights reserved.

Role of surfactant protein-A (SP-A) in lung injury in response to acute ozone exposure of SP-A deficient mice

International Nuclear Information System (INIS)

Haque, Rizwanul; Umstead, Todd M.; Ponnuru, Padmavathi; Guo Xiaoxuan; Hawgood, Samuel; Phelps, David S.; Floros, Joanna

2007-01-01

Millions are exposed to ozone levels above recommended limits, impairing lung function, causing epithelial damage and inflammation, and predisposing some individuals to pneumonia, asthma, and other lung conditions. Surfactant protein-A (SP-A) plays a role in host defense, the regulation of inflammation, and repair of tissue damage. We tested the hypothesis that the lungs of SP-A(-/-) (KO) mice are more susceptible to ozone-induced damage. We compared the effects of ozone on KO and wild type (WT) mice on the C57BL/6 genetic background by exposing them to 2 parts/million of ozone for 3 or 6 h and sacrificing them 0, 4, and 24 h later. Lungs were subject to bronchoalveolar lavage (BAL) or used to measure endpoints of oxidative stress and inflammation. Despite more total protein in BAL of KO mice after a 3 h ozone exposure, WT mice had increased oxidation of protein and had oxidized SP-A dimers. In KO mice there was epithelial damage as assessed by increased LDH activity and there was increased phospholipid content. In WT mice there were more BAL PMNs and elevated macrophage inflammatory protein (MIP)-2 and monocyte chemoattractant protein (MCP)-1. Changes in MIP-2 and MCP-1 were observed in both KO and WT, however mRNA levels differed. In KO mice MIP-2 mRNA levels changed little with ozone, but in WT levels they were significantly increased. In summary, several aspects of the inflammatory response differ between WT and KO mice. These in vivo findings appear to implicate SP-A in regulating inflammation and limiting epithelial damage in response to ozone exposure

Type-IVC Secretion System: A Novel Subclass of Type IV Secretion System (T4SS) Common Existing in Gram-Positive Genus Streptococcus

Science.gov (United States)

Chen, Chen; Gao, George F.

2012-01-01

A growing number of pathogens are being found to possess specialized secretion systems which they use in various ways to subvert host defenses. Type IV secretion system (T4SS) is one of versatile secretion systems essential for the virulence and even survival of some bacteria species, and they enable the secretion of protein and DNA substrates across the cell envelope. T4SS was once believed to be present only in Gram-negative bacteria. In this study, we present evidence of a new subclass of T4SS, Type-IVC secretion system and indicate its common existence in the Gram-positive bacterial genus Streptococcus. We further identified that VirB1, VirB4, VirB6 and VirD4 are the minimal key components of this system. Using genome comparisons and evolutionary relationship analysis, we proposed that Type-IVC secretion system is movable via transposon factors and mediates the conjugative transfer of DNA, enhances bacterial pathogenicity, and could cause large-scale outbreaks of infections in humans. PMID:23056296

Low PAPP-A in the first trimester is associated with reduced fetal growth rate prior to gestational week 20

DEFF Research Database (Denmark)

Salvig, J D; Kirkegaard, I; Winding, Trine Nøhr

2010-01-01

To evaluate the association between maternal pregnancy-associated plasma protein-A (PAPP-A) and fetal growth from the first to the second trimester.......To evaluate the association between maternal pregnancy-associated plasma protein-A (PAPP-A) and fetal growth from the first to the second trimester....

Characterization of Mycoplasma penetrans and Mycoplasma fermentans immunodominant proteins Caracterização de proteinas imunodominantes de Mycoplasma penetrans e Mycoplasma fermentans

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Juliana Bruder

2005-06-01

lipoproteica cujo pequeno genoma sintetiza a maioria das moléculas necessárias para crescimento e replicação. Dentre as dezesseis espécies isoladas do homem, Mycoplasma pneumoniae, agente causador da pneumonia atípica primária, e as espécies do trato urogenital como Mycoplasma hominis,Ureaplasma urealyticum e Ureaplasma parvum têm definido seu papel patogênico. M. penetrans e M. fermentans, espécies associadas ao HIV, têm sido investigadas principalmente em laboratórios de pesquisa. Considerando a importância dos micoplasmas nas doenças humanas e a peculiar variação antigênica observada em tais espécies, foram caracterizadas, neste estudo, as lipoproteínas associadas a membranas (LAMP de Mycoplasma penetrans e Mycoplasma fermentans. Para definir peptídeos com possível valor diagnóstico, empregamos as técnicas de ELISA e de Western blot usando soros de gestantes cujas amostras cervicais foram positivas por PCR. Por meio do ELISA foram observados anticorpos IgG anti-LAMP-M. fermentans em 57,5% e IgM em 74,5% das amostras. As três amostras PCR positivas para M. penetrans apresentaram anticorpos IgG anti-LAMP-M. penetrans e uma amostra positiva para IgM. IgA não foi detectada em nenhuma das espécies. A análise da LAMP, por Western blot, revelou como principais proteinas imunoreativas: 35, 38, 42, 61 and 103 kDa para M. penetrans e 29, 38, 41, 61, 78 and 95 kDa de M. fermentans. Dentre estas podemos considerar p35 específica para M. penetrans e p29, M. fermentans.Tais proteinas são promissoras como marcadores em diagnóstico.

Nucleic Acids and Protein Metabolism of Bone Marrow Cells Studied by Means of Tritiumlabelled Precursors; Etude du Metabolisme des Acides Nucleiques et des Proteines dans les Cellules de la Moelle Osseuse, a l'Aide de Precurseurs Trities; 0418 0437 0443 0414 ; Estudio con Ayuda de Precursores Tritiados del Metabolismo de los acidos Nucleicos y de las Proteinas en las Celulas de la Medula Osea;

Energy Technology Data Exchange (ETDEWEB)

Gavosto, F. [Istituto di Clinica Medica Generale e Centro di Studi Fisico-Biologici, Universita de Turin, Turin (Italy)

1962-02-15

. L'auteur examine ensuite la diminution et la dissociation du metabolisme de l'ARN et des proteines, dans les cellules de sujets atteints de leucemie aiguee, par rapport aux anomalies bien connues que presente le processus de maturation de ces cellules. (author) [Spanish] El autor examina las ventajas que presenta el empleo de los compuestos marcados con tritio en la tecnica autorradiografica. En efecto, los electrones emitidos por el tritio tienen una energia maxima de 0,018 MeV, que corresponde, aproximadamente, a un alcance de 1 miera en una emulsion fotografica y permiten, por lo tanto, lograr el maximo poder de resolucion al nivel celular y subcelular. Ello es particularmente util para estudiar fenomenos metabolicos en tejidos que se componen, como en el caso de la medula osea, de celulas de varios tipos en diversos estados de diferenciacion. Esta tecnica se ha aplicado al estudio del metabolismo de los acidos nucleicos y de las proteinas en las celulas normales y leucemicas de la medula osea. El metabolismo del acido desoxirribonucleico se ha estudiado utilizando un precursor especifico, la timidina-{sup 3}H. Comparando los elementos normales y leucemicos que han alcanzado un grado de madurez analogo, se han observado diferencias significativas en el porcentaje de celulas marcadas. Especialmente, en los casos de leucemia aguda, se ha comprobado que la incorporacion de timidina en las celulas leucemicas disminuye muy notablemente. Se estima que estos resultados constituyen una prueba de que la capacidad de proliferacion de estas celulas disminuye en comparacion con la de los mieloblastos normales. Por este mismo procedimiento, se ha estudiado el metabolismo del acido ribonucleico y de las proteinas, utilizando como precursores uridina-{sup 3}H, leucina-{sup 3}H y fenilalanina-{sup 3}H. Actualmente, en biologia celular se admite sin reservas la existencia de una estrecha relacion entre el metabolismo del acido ribonucleico y el de las proteinas. Ademas, se ha

Brucella Modulates Secretory Trafficking via Multiple Type IV Secretion Effector Proteins

Science.gov (United States)

Myeni, Sebenzile; Child, Robert; Ng, Tony W.; Kupko, John J.; Wehrly, Tara D.; Porcella, Stephen F.; Knodler, Leigh A.; Celli, Jean

2013-01-01

The intracellular pathogenic bacterium Brucella generates a replicative vacuole (rBCV) derived from the endoplasmic reticulum via subversion of the host cell secretory pathway. rBCV biogenesis requires the expression of the Type IV secretion system (T4SS) VirB, which is thought to translocate effector proteins that modulate membrane trafficking along the endocytic and secretory pathways. To date, only a few T4SS substrates have been identified, whose molecular functions remain unknown. Here, we used an in silico screen to identify putative T4SS effector candidate proteins using criteria such as limited homology in other bacterial genera, the presence of features similar to known VirB T4SS effectors, GC content and presence of eukaryotic-like motifs. Using β-lactamase and CyaA adenylate cyclase reporter assays, we identified eleven proteins translocated into host cells by Brucella, five in a VirB T4SS-dependent manner, namely BAB1_0678 (BspA), BAB1_0712 (BspB), BAB1_0847 (BspC), BAB1_1671 (BspE) and BAB1_1948 (BspF). A subset of the translocated proteins targeted secretory pathway compartments when ectopically expressed in HeLa cells, and the VirB effectors BspA, BspB and BspF inhibited protein secretion. Brucella infection also impaired host protein secretion in a process requiring BspA, BspB and BspF. Single or combined deletions of bspA, bspB and bspF affected Brucella ability to replicate in macrophages and persist in the liver of infected mice. Taken together, these findings demonstrate that Brucella modulates secretory trafficking via multiple T4SS effector proteins that likely act coordinately to promote Brucella pathogenesis. PMID:23950720

Reconstruction of multispectral light field (5d plenoptic function based on compressive sensing with colored coded apertures from 2D projections

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Kareth Marcela León-López

2016-01-01

Full Text Available Los sistemas de adquisición de información espacio – angular en 4D o campos de luz, han avanzado en la última década debido a la inclusión de aperturas codificadas en el camino óptico del sistema. Dichas aperturas, modulan la luz codificando la información antes de ser capturada. Tradicionalmente, estas aperturas codificadas son aperturas binarias, es decir, bloquean o dejan pasar los rayos de luz en la dimensión espacial haciendo que se capture información dispersa de la escena. En este trabajo, se reemplazan las aperturas binarias por aperturas codificadas de color, que además de modular la luz espacialmente lo hacen espectralmente. De esta forma, es posible adquirir campos de luz en múltiples longitudes de onda y aumentar su resolución espectral. La importancia de obtener información en el espectro radica en que es posible capturar características que no están presentes en el rango visible del espectro electromagnético. En este artículo, se propone un algoritmo que simula la adquisición de una imagen de campo de luz usando aperturas codificadas de color. El sistema propuesto aprovecha la redundancia de información que se presenta en la escena basándose en la teoría del muestreo compresivo, así, solo se captura una señal dispersa de la escena. La imagen multidimensional es recuperada a partir de dicha señal mediante un algoritmo de reconstrucción. Los resultados de simulación muestran la calidad de reconstrucción del campo de luz multiespectral. La calidad de las reconstrucciones obtenidas es de alrededor de 25 dB en PSNR.

ESTUDIO DE LA ADSORCIÓN DE PROTEÍNAS SOBRE SUPERFICIES DE ACIDO POLILÁCTICO MEDIANTE TÉCNICAS GRAVIMÉTRICAS Y ELECTROQUÍMICAS

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HUGO ARMANDO ESTUPIÑAN DURAN

2011-01-01

Full Text Available Metodos electroquimicos fueron utilizados para promover y evaluar la adsorcion orientada de albumina y colageno sobre superficies polimericas de acido polilactico (PLA mediante la aplicacion de un sobrepotencial anodico en un rango de 0 V a 0,45 V. La adsorcion fueestudiada usando mediciones simultaneas de espectroscopia de impedancia electroquimica (EIE y microbalanza de cristal de cuarzo (QCM, en un rango de temperaturas de 25°C a 45°C, con variaciones en proteina entre 5 y 20 ¿Êg/ml en solucion buffer de fosfato de pH 7,4. Con el aumento de la concentracion de proteinas en el electrolito, disminuye la resistencia a la transferencia de carga, exhibiendo asi una mayor adsorcion sobre la superficie, ademas de aumentar la magnitud de los cambios de masa. Se mejoro la hidrofilicidad del recubrimiento, mostrando una mayor afinidad con las soluciones de colageno y por lo tanto un aumento en la biocompatibilidad debido a la activacion superficial.

ESTUDIO PRELIMINAR DE LA FITOHEMOAGLUTININA DE LA CANAVALIA BRASILIENSIS

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Gerardo Perez Gómez

2010-09-01

Full Text Available Se establecio la presencia de una lectina en semillas de Canavalia brasiliensis. Esta proteina que es una globulina, presenta una alta actividad aglutinante respecto a eritrocitos equinos y caninos; esta aglutinacion es inhibida considerable mente por melezitosa (7mg/ml y en menor grado por sacarosa, fructosa y glucosa. Los ensayos realizados con eritrocitos humanos, bovinos o de carnero, demuestra que la lectina es capaz de aglutinarlos solo despues de una tripsinizacion.

A bipartite signal mediates the transfer of type IV secretion substrates of Bartonella henselae into human cells.

Science.gov (United States)

Schulein, Ralf; Guye, Patrick; Rhomberg, Thomas A; Schmid, Michael C; Schröder, Gunnar; Vergunst, Annette C; Carena, Ilaria; Dehio, Christoph

2005-01-18

Bacterial type IV secretion (T4S) systems mediate the transfer of macromolecular substrates into various target cells, e.g., the conjugative transfer of DNA into bacteria or the transfer of virulence proteins into eukaryotic host cells. The T4S apparatus VirB of the vascular tumor-inducing pathogen Bartonella henselae causes subversion of human endothelial cell (HEC) function. Here we report the identification of multiple protein substrates of VirB, which, upon translocation into HEC, mediate all known VirB-dependent cellular changes. These Bartonella-translocated effector proteins (Beps) A-G are encoded together with the VirB system and the T4S coupling protein VirD4 on a Bartonella-specific pathogenicity island. The Beps display a modular architecture, suggesting an evolution by extensive domain duplication and reshuffling. The C terminus of each Bep harbors at least one copy of the Bep-intracellular delivery domain and a short positively charged tail sequence. This biparte C terminus constitutes a transfer signal that is sufficient to mediate VirB/VirD4-dependent intracellular delivery of reporter protein fusions. The Bep-intracellular delivery domain is also present in conjugative relaxases of bacterial conjugation systems. We exemplarily show that the C terminus of such a conjugative relaxase mediates protein transfer through the Bartonella henselae VirB/VirD4 system into HEC. Conjugative relaxases may thus represent the evolutionary origin of the here defined T4S signal for protein transfer into human cells.

Longer time-to-pregnancy in spontaneously conceived pregnancies is associated with lower PAPP-A and free β-hCG in first trimester screening for Down syndrome

DEFF Research Database (Denmark)

Kirkegaard, I; Uldbjerg, N; Tabor, A

2014-01-01

OBJECTIVE: The aim of this study was to investigate whether subfertility, measured as longer time-to-pregnancy (TTP) in spontaneously conceived pregnancies, affects the first trimester levels of pregnancy-associated plasma protein-A (PAPP-A) and free beta-human chorionic gonadotrophin (β-hCG) and......OBJECTIVE: The aim of this study was to investigate whether subfertility, measured as longer time-to-pregnancy (TTP) in spontaneously conceived pregnancies, affects the first trimester levels of pregnancy-associated plasma protein-A (PAPP-A) and free beta-human chorionic gonadotrophin (β...

Detección de linfocitos T citotóxicos específicos anti-53 en pacientes con cáncer de mama

OpenAIRE

Álvarez Busto, Iñaki; Tres Sánchez, Alejandro; Mayordomo Cámara, José Ignacio

2009-01-01

El gen P53 es el más frecuentemente mutado en tumores humanos ( 50%). Su proteina producto, la proteina P53, se acumula cuando sufre una mutación. Esta circunstancia posibilita su presentación al sistema inmunológico y el desarrollo de una respuesta celular específica medida por linfocitos T CD8. En este trabajo por primera vez, ha sido posible desarrollar la existencia de una respuesta inmune celular específica "in vivo" en pacientes con cancer de mama. Las implicaciones de este hallazgo par...

First-trimester risk calculation for trisomy 13, 18, and 21: comparison of the screening efficiency between 2 locally developed programs and commercial software

DEFF Research Database (Denmark)

Sørensen, Steen; Momsen, Günther; Sundberg, Karin

2011-01-01

Reliable individual risk calculation for trisomy (T) 13, 18, and 21 in first-trimester screening depends on good estimates of the medians for fetal nuchal translucency thickness (NT), free β-subunit of human chorionic gonadotropin (hCGβ), and pregnancy-associated plasma protein-A (PAPP-A) in mate......Reliable individual risk calculation for trisomy (T) 13, 18, and 21 in first-trimester screening depends on good estimates of the medians for fetal nuchal translucency thickness (NT), free β-subunit of human chorionic gonadotropin (hCGβ), and pregnancy-associated plasma protein-A (PAPP...

Estudo do comportamento acido-base de proteinas no sistema DMSO-Agua

OpenAIRE

Ivo Milton Raimundo Junior

1989-01-01

Resumo: Neste trabalho foi estudado o comportamento ácido-base da lisozima e ovalbumina no sistema DMSO-água (7,0: 0,8 ml) através de titulações termométrica catalítica (TTC) e potenciométrica. Na TTC foi utilizada acrilonitrila (4,0 ml) como indicador termométrico, sendo determinados 23,6±0,7 e 47,6±0,6 .grupos ácidos por moI de lisozima e ovalbumina, respectivamente. A comparação desses resultados com os obtidos por titulação potenciométrica das proteínas desnaturadas em uréia mostrou que o...

Caracterización de la actividad alergénica y enzimática de extractos somáticos producidos a partir de cultivos in vitro del ácaro Dermatophagoide farinae

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Dary Luz Mendoza Meza

2011-01-01

Full Text Available Introduccion: El Dermatophagoides fainae (DF es un acaro cosmopolita presente en el polvo intradomiciliario. Este acaro es fuente importante de aereo-alergenos desencadenantes de asma y rinitis alergica. Objetivo: El proposito de este trabajo fue la produccion y caracterizacion de extractos alergenicos para uso en diagnostico alergologico y terapia. Metodos: Los extractos se prepararon a partir de cultivos puros in vitro de acaros. Las proteinas del acaro fueron extraidas mediante lisis osmotica. La concentracion de proteinas se determino por microensayo de Bradford y el perfil electroforetico fue identificado por SDS-PAGE. La actividad enzimatica fue evaluada con el estuche comercial Api Zym (Biomeriux R y la actividad alergenica por inmunoensayo de Dot Blot. Resultados: Se prepararon cinco lotes de extractos. La media del peso seco de material extraido fue 37,04 mg/gramo de acaros ( DE= 11,3 y la media de la cantidad de proteina fue 212,4 ¿Êg/mg de peso seco (DE= 33,3. El perfil SDS-PAGE mostro la presencia de 8 bandas comunes (21-109 kDa. Todos los extractos presentaron actividad enzimatica hidrolasa. Tambien se demostro actividad tripsina, alfa-amilasa y alfa-quimiotripsina, correspondientes a los alergenos de los acaros del grupo 3, 4 y 6 respectivamente. Todos los extractos tuvieron proteinas con capacidad de union a los anticuerpos IgE. Conclusion: Aqui presentamos la produccion de extractos del acaro DF, con actividad alergenica. Estos resultados fundamentaran la produccion a escala de extractos alergenicos de acaros desencadenantes de enfermedades alergicas en el humano, con fines diagnosticos y de aplicacion terapeutica.

Evolution of the gene translation machinery and its applications to drug discovery

OpenAIRE

Novoa Pardo, Eva Maria

2012-01-01

[spa] La traducción de proteinas es un proceso central en todas las células, en el cual el ARN mensajero es descodificado en el ribosoma para producir una cadena aminoacídica, que después se plegará dando lugar a una proteina activa. Este proceso está facilitado por los ARN de transferencia (ARNt), que llevan unidos covalentemente amino ácidos específicos. La unión precisa de cada amino ácido a su ARNt está catalizada por las aminoacil-ARNt sintetasas, y por tanto, estas enzimas son las respo...

Identification of VceA and VceC, two members of the VjbR regulon that are translocated into macrophages by the Brucella type IV secretion system

NARCIS (Netherlands)

de Jong, Maarten F.; Sun, Yao-Hui; den Hartigh, Andreas B.; van Dijl, Jan Maarten; Tsolis, Renee M.

2008-01-01

Survival and replication inside host cells by Brucella spp. requires a type IV secretion system (T4SS), encoded by the virB locus. However, the identity of the molecules secreted by the T4SS has remained elusive. We hypothesized that proteins translocated by the T4SS would be co-regulated with the

Gastric epithelial expression of IL-12 cytokine family in Helicobacter pylori infection in human: is it head or tail of the coin?

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Fadi Al-Sammak

Full Text Available Recently, there has been a growing interest in an expanding group of cytokines known as "IL-12 family". The so far gained knowledge about these cytokines, as crucial playmakers in mucosal immunity, has not yet been sufficiently investigated in the context of Helicobacter pylori infection. All genes encoding the monomeric components of these cytokines and their corresponding receptors were examined in gastric epithelial cell lines (AGS and MKN-28 after being infected with 4 H. pylori strains: BCM-300, P1 wild-type, and P1-derived isogenic mutants lacking cytotoxin-associated gene A (cagA or virulence gene virB7 (multiplicity of infection=50. Both infected and uninfected samples were analyzed after 24h and 48h using real-time quantitative polymerase chain reaction (RT-qPCR. Gene expression analysis demonstrated a strong upregulation of IL23A (encodes p19 by infection, whereas IL23R, Epstein-Barr virus-induced gene 3 (EBI3, IL6ST, IL12A, and IL27RA were found to be expressed, but not regulated, or to a lesser extent. Transcripts of IL12RB2, IL12B, IL12RB1, and IL27A were not detected. Interestingly, P1 resulted in stronger alterations of expression than CagA mutant and BCM-300, particularly for IL23A (59.7-fold versus 32.4- and 6.7-fold, respectively in AGS after 48h, P<.05, whereas no changes were seen with VirB7 mutant. In a proof-of-principle experiment, we demonstrated epithelial-derived expression of IL-12, p19, and Ebi3 in gastric mucosa of gastritis patients using immunohistochemistry (IHC. Unlike IL-12 and Ebi3, increased immunostaining of p19 was observed in H. pylori gastritis. Herein, we highlight the potential role of gastric epithelial cells in mucosal immunity, not only because they are predominant cell type in mucosa and initial site of host-bacterial interaction, but also as a major contributor to molecules that are thought to be primarily expressed by immune cells so far. Of these molecules, p19 was the most relevant one to H

Detection of virulence-associated genes in Brucella melitensis ...

African Journals Online (AJOL)

Ibrahim Eldaghayes

2018-03-20

Mar 20, 2018 ... isolated from goats. This discrepancies may indicate that B. melitensis field strains prevailing in Egypt are more virulent than the strains of B. melitensis isolated from caprines in Iran. As, it was emphasized that the. T4SS of Brucella encoded by the virB operon is a major virulence factor (Delrue et al., 2005).

Single-pixel optical sensing architecture for compressive hyperspectral imaging

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Hoover Fabián Rueda-Chacón

2014-01-01

Full Text Available Los sistemas de sensado de imágenes espectrales (CSI capturan información tridimensional (3D de una escena usando mediciones codificadas en dos dimensiones (2D. Estas mediciones son procesadas posteriormente por un algoritmo de optimización para obtener una estimación de la información tridimensional. La calidad de las reconstrucciones obtenidas depende altamente de la resolución del detector, cuyo costo aumenta exponencialmente a mayor resolución exhiba. Así, reconstrucciones de alta resolución son requeridas, pero a bajo costo. Este artículo propone una arquitectura óptica de sensado compresivo que utiliza un único pixel como detector para la captura y reconstrucción de imágenes hiperespectrales. Esta arquitectura óptica depende del uso de múltiples capturas de imágenes procesadas por medio de dos aperturas codificadas que varían en cada toma, y un elemento de dispersión. Diferentes simulaciones con 2 bases de datos distintas muestran resultados promisorios que permiten reconstruir una imagen hiperespectral utilizando tan solo el 30% de los vóxeles de la imagen original.

A bacterial toxin-antitoxin module is the origin of inter-bacterial and inter-kingdom effectors of Bartonella.

Science.gov (United States)

Harms, Alexander; Liesch, Marius; Körner, Jonas; Québatte, Maxime; Engel, Philipp; Dehio, Christoph

2017-10-01

Host-targeting type IV secretion systems (T4SS) evolved from conjugative T4SS machineries that mediate interbacterial plasmid transfer. However, the origins of effectors secreted by these virulence devices have remained largely elusive. Previous work showed that some effectors exhibit homology to toxins of bacterial toxin-antitoxin modules, but the evolutionary trajectories underlying these ties had not been resolved. We previously reported that FicT toxins of FicTA toxin-antitoxin modules disrupt cellular DNA topology via their enzymatic FIC (filamentation induced by cAMP) domain. Intriguingly, the FIC domain of the FicT toxin VbhT of Bartonella schoenbuchensis is fused to a type IV secretion signal-the BID (Bep intracellular delivery) domain-similar to the Bartonella effector proteins (Beps) that are secreted into eukaryotic host cells via the host-targeting VirB T4SS. In this study, we show that the VbhT toxin is an interbacterial effector protein secreted via the conjugative Vbh T4SS that is closely related to the VirB T4SS and encoded by plasmid pVbh of B. schoenbuchensis. We therefore propose that the Vbh T4SS together with its effector VbhT represent an evolutionary missing link on a path that leads from a regular conjugation system and FicTA toxin-antitoxin modules to the VirB T4SS and the Beps. Intriguingly, phylogenetic analyses revealed that the fusion of FIC and BID domains has probably occurred independently in VbhT and the common ancestor of the Beps, suggesting parallel evolutionary paths. Moreover, several other examples of TA module toxins that are bona fide substrates of conjugative T4SS indicate that their recruitment as interbacterial effectors is prevalent and serves yet unknown biological functions in the context of bacterial conjugation. We propose that the adaptation for interbacterial transfer favors the exaptation of FicT and other TA module toxins as inter-kingdom effectors and may thus constitute an important stepping stone in the

A bacterial toxin-antitoxin module is the origin of inter-bacterial and inter-kingdom effectors of Bartonella.

Directory of Open Access Journals (Sweden)

Alexander Harms

2017-10-01

Full Text Available Host-targeting type IV secretion systems (T4SS evolved from conjugative T4SS machineries that mediate interbacterial plasmid transfer. However, the origins of effectors secreted by these virulence devices have remained largely elusive. Previous work showed that some effectors exhibit homology to toxins of bacterial toxin-antitoxin modules, but the evolutionary trajectories underlying these ties had not been resolved. We previously reported that FicT toxins of FicTA toxin-antitoxin modules disrupt cellular DNA topology via their enzymatic FIC (filamentation induced by cAMP domain. Intriguingly, the FIC domain of the FicT toxin VbhT of Bartonella schoenbuchensis is fused to a type IV secretion signal-the BID (Bep intracellular delivery domain-similar to the Bartonella effector proteins (Beps that are secreted into eukaryotic host cells via the host-targeting VirB T4SS. In this study, we show that the VbhT toxin is an interbacterial effector protein secreted via the conjugative Vbh T4SS that is closely related to the VirB T4SS and encoded by plasmid pVbh of B. schoenbuchensis. We therefore propose that the Vbh T4SS together with its effector VbhT represent an evolutionary missing link on a path that leads from a regular conjugation system and FicTA toxin-antitoxin modules to the VirB T4SS and the Beps. Intriguingly, phylogenetic analyses revealed that the fusion of FIC and BID domains has probably occurred independently in VbhT and the common ancestor of the Beps, suggesting parallel evolutionary paths. Moreover, several other examples of TA module toxins that are bona fide substrates of conjugative T4SS indicate that their recruitment as interbacterial effectors is prevalent and serves yet unknown biological functions in the context of bacterial conjugation. We propose that the adaptation for interbacterial transfer favors the exaptation of FicT and other TA module toxins as inter-kingdom effectors and may thus constitute an important stepping

Gender impact on first trimester markers in Down syndrome screening

DEFF Research Database (Denmark)

Larsen, Severin Olesen; Wøjdemann, Karen R; Shalmi, Anne-Cathrine

2002-01-01

The influence of fetal gender on the level in the first trimester of the serological markers alpha-fetoprotein (AFP), pregnancy-associated plasma protein-A (PAPP-A) and free beta human chorionic gonadotropin (betahCG) and on nuchal translucency is described for 2637 singleton pregnancies with nor......The influence of fetal gender on the level in the first trimester of the serological markers alpha-fetoprotein (AFP), pregnancy-associated plasma protein-A (PAPP-A) and free beta human chorionic gonadotropin (betahCG) and on nuchal translucency is described for 2637 singleton pregnancies...... with normal outcome. Mean log MoM values for pregnancies with female and male fetuses were calculated using regression of log marker values on gestational age expressed as crown rump length and on maternal weight. A pronounced gender impact was found for free betahCG, being 16% higher for female than for male...

SP-A-enriched surfactant for treatment of rat lung transplants with SP-A deficiency after storage and reperfusion

NARCIS (Netherlands)

Erasmus, ME; Hofstede, GJH; Petersen, AH; Batenburg, JJ; Haagsman, HP; Oetomo, SB; Prop, J

2002-01-01

Background. The function of pulmonary surfactant is affected by lung transplantation, contributing to impaired lung transplant function. A decreased amount of surfactant protein-A (SP-A) after reperfusion is believed to contribute to the impaired surfactant function. Surfactant treatment has been

Insulin-Like Growth Factor Binding Protein 4 Fragments Provide Incremental Prognostic Information on Cardiovascular Events in Patients With ST-Segment Elevation Myocardial Infarction

DEFF Research Database (Denmark)

Hjortebjerg, Rikke; Lindberg, Søren; Pedersen, Sune

2017-01-01

BACKGROUND: Fragments of insulin-like growth factor binding protein 4 (IGFBP-4) are potential new biomarkers for cardiac risk assessment. The fragments are generated on specific cleavage by pregnancy-associated plasma protein-A, which exerts proatherogenic activity. This study investigated the pr...

Improved first-trimester Down syndrome screening performance by lowering the false-positive rate: a prospective study of 9941 low-risk women

DEFF Research Database (Denmark)

Wøjdemann, K R; Shalmi, A C; Christiansen, M

2005-01-01

OBJECTIVE: To determine the performance of screening for Down syndrome (DS) and other major chromosomal abnormalities using nuchal translucency (NT), free beta-human chorionic gonadotropin (beta-hCG) and pregnancy-associated plasma protein-A (PAPP-A) in a prospective study of a non...

First trimester screening for intra-uterine growth restriction and early-onset pre-eclampsia

NARCIS (Netherlands)

Vandenberghe, G.; Mensink, I.; Twisk, J.W.; Blankenstein, M.A.; Heijboer, A.C.; van Vugt, J.M.

2011-01-01

Objective: To assess first trimester placental growth factor (PlGF) and pregnancy-associated plasma protein-A (PAPP-A) as screening markers for early-onset pre-eclampsia (PE) and intra-uterine growth restriction (IUGR). Methods: PlGF concentration was retrospectively measured in first trimester

Optimizing the Immuno-Surface Characteristics for Bio-Sensors and Filters Through Modeling and Experiments

Science.gov (United States)

2005-06-01

immobilization of antibodies o Adsorbed, aminophase, heterobifunctional crosslinkers (GMBS, BMPS, EMCS) o GMBS attaches the most antibodies o ProteinA ...play a role in getting the antigen close enough to the immuno-surface to potentially interact as well as the short range molecular forces that

A prospective study evaluating the performance of first trimester combined screening for trisomy 21 using repeated sampling of the maternal serum markers PAPP-A and free β-hCG

DEFF Research Database (Denmark)

Ekelund, Charlotte Kvist; Wright, Dave; Ball, Susan

2012-01-01

OBJECTIVE: To prospectively evaluate the performance of first-trimester combined screening for trisomy 21 using the biochemical markers pregnancy-associated plasma protein-A (PAPP-A) and free beta-human chorionic gonadotropin (free β-hCG) obtained before and at the time of the nuchal translucency...

First trimester screening for intra-uterine growth restriction and early-onset pre-eclampsia

NARCIS (Netherlands)

Vandenberghe, G.; Mensink, I.; Twisk, J. W. R.; Blankenstein, M. A.; Heijboer, A. C.; van Vugt, J. M. G.

2011-01-01

To assess first trimester placental growth factor (PlGF) and pregnancy-associated plasma protein-A (PAPP-A) as screening markers for early-onset pre-eclampsia (PE) and intra-uterine growth restriction (IUGR). PlGF concentration was retrospectively measured in first trimester serum specimens of 23

Multivariate analysis identifies the estradiol level at ovulation triggering as an independent predictor of the first trimester pregnancy-associated plasma protein-A level in IVF/ICSI pregnancies.

Science.gov (United States)

Giorgetti, C; Vanden Meerschaut, F; De Roo, C; Saunier, O; Quarello, E; Hairion, D; Penaranda, G; Chabert-Orsini, V; De Sutter, P

2013-10-01

Can independent predictors of pregnancy-associated plasma protein-A (PAPP-A) levels be identified in a group of women who conceived following IVF/ICSI? The significantly decreased PAPP-A level in IVF and ICSI pregnancies compared with non-IVF/ICSI pregnancies was correlated strongly with the serum estradiol (E2) level at ovulation triggering. The first trimester prenatal combined screening test for fetal aneuploidies in pregnancies conceived following assisted reproduction techniques (ART) is complicated by an alteration of the maternal biomarkers free β-hCG and PAPP-A, causing a higher false-positive rate compared with pregnancies which are conceived naturally. The use of controlled ovarian stimulation prior to IVF/ICSI is suggested to be the principle reason for these alterations of biomarkers in ART pregnancies. Between January 2010 and December 2011, 1474 women who conceived naturally and 374 women who conceived following IVF (n = 89), ICSI (n = 204) or intrauterine insemination (IUI, n = 81) were included in this retrospective study. Only singleton pregnancies were eligible for this study. For all women, serum analysis was performed in the same clinical laboratory. Measurement of nuchal translucency (NT) thickness was performed by four physicians belonging to the same infertility centre. First-trimester combined screening test of aneuploidy parameters (maternal age, PAPP-A and free β-hCG, NT thickness) were compared between non-ART and ART (IVF, ICSI and IUI) singleton pregnancies. Next, a minimal threshold E2 level at ovulation triggering was suggested for IVF/ICSI pregnancies above which the PAPP-A levels were significantly decreased compared with non-ART pregnancies. Finally, a multivariate analysis was performed to reveal independent predictors of PAPP-A level in IVF/ICSI pregnancies. We showed a decrease of the multiple of the median (MoM) PAPP-A level in IVF and ICSI singleton pregnancies compared with non-ART singleton pregnancies (P IVF and ICSI

Detection of triploidy at 11-14 weeks of gestation

DEFF Research Database (Denmark)

Engelbrechtsen, Line; Brøndum-Nielsen, Karen; Ekelund, Charlotte Kvist

2013-01-01

included: Nuchal translucency (NT), maternal serum free β-human chorionic gonadotrophin (β-hCG) and pregnancy associated plasma protein-A (PAPP-A). In all triploid fetuses FTS parameters were re-evaluated. Karyotypes were established by invasive testing (CVS or AC) or post abortem and obtained from...

First trimester PAPP-A2, PAPP-A and hCGβ in small-for-gestational-age pregnancies

DEFF Research Database (Denmark)

Hansen, Young Bae; Myrhøj, Vibeke; Jørgensen, Finn Stener

2016-01-01

BACKGROUND: Pregnancy-associated plasma protein-A2 (PAPP-A2) is a recently discovered protease that cleaves a subset of insulin-like growth factor binding proteins (IGFBP). The molecular function suggests its involvement in the IGF system that is vital for fetal growth and development. Our...

La gastroenterología en Venezuela durante el primer quinquenio del siglo XXI (I) causas hepato-biliares: Consideraciones sobre mortalidad

OpenAIRE

Lizarzabal de Belloso, Maribel

2007-01-01

Los perfiles de mortalidad orientan sobre las prioridades necesarias en la prevención de enfermedades. En Venezuela se publican Anuarios de Mortalidad oficiales basados en Certificados de Defunción (distribuidos por Direcciones Regionales de Epidemiología/DVE/DEAE/MSDS) que contienen causas de muerte codificadas, siguiendo lineamientos internacionales CIE-10 de la OMS. Aún cuando estas cifras están a disposición del público, consideramos importante en este trabajo descriptivo-retrospectivo ex...

Efeitos da dieta proteica na cicatrização de fraturas distais de fêmur imobilizadas com pinos intramedulares em cão

Directory of Open Access Journals (Sweden)

Silveira Iandara Silva

1997-01-01

Full Text Available No presente experimento vinte cães sem raça definida, pesando em média 6kg, com idade variando entre 4 e 12 anos, vindos do Biotério Central da Universidade Federal de Santa Maria (UFSM, foram distribuidos em dois grupos com 10 animais, cada grupo dividido em dois subgrupos com 5 cães, denominados 1A, 1B, 2A e 2B. Os animais do grupo 1 receberam tratamento por 90 dias, e os do grupo 2 por 60 dias após a cirurgia. O subgrupo A correspondem a tratamento com ração crescimento contendo 27% de proteina bruta e o subgrupo B ração manutenção com 21% de proteina bruta. Todos os cães sofreram fratura distal de fêmur, experimental, reduzidas com dois pinos intramedulares, introduzidos através da superfície articular da tróclea. Os cães ficaram confinados em canis individuais até a remoção dos pontos e em canis comuns para no máximo 5 cães até o término do experimento. Foi feita avaliação clínica, radiográfica e histológica da evolução da cicatrização óssea a qual demonstrou que o tratamento com ração com maior teor de proteina proporcionou melhor regeneração e que a técnica de osteossíntese utilizada manteve estabilidade na linha de fratura.

Detección y expresión de una proteína de unión a calmodulina en Plasmodium falciparum

Directory of Open Access Journals (Sweden)

Angela P. Guerra

2001-03-01

Full Text Available Se produjo un anticuerpo monoclonal a partir de proteinas de unión a calmodulina (PUCaM aisladas por cromatografia de afinidad. Utilizando el inmunoensayo Western blot se detectó una proteina de 30 kDa en extractos de glóbulos rojos parasitados con Plasmodium falciparum. Esta señal no se observó en extractos de eritrocitos no infectados. adicionalmente, este anticuerpo presentó una reacción con dos bandas de alto peso molecular localizadas en 260 y 240 kDa, siendo mucho más intensa la señal para 240 kDa. Estas mismas bandas fueron reconocidas por un anticuerpo policlonal anti-espectrina. Lo anterior implica que el anticuerpo monoclonal 1G3 presenta una reacción cruzada con las cadenas de la espectrina y sugiere la presencia de un epítope similar o una estructura estrechamente relacionada en ambas proteinas. Se estudió la expresión de esta proteína durante el ciclo de desarrollo del parásito y se pudo definir su presencia en las fases tardías del ciclo. La cantidad de la PUCaM de 30 kDa no sólo aumenta de manera absoluta, sino también relativamente con relación a las demás proteínas del parásito. El hecho de que se presente señal en los estadios avanzados de desarrollo y no en el estadio de anillos está indicando que tiene un patrón de expresión diferencial durante el desarrollo del parásito.

Functional Characterization of CENP-A Post-Translational Modifications in Chromosome Segregation

Science.gov (United States)

2016-09-01

Yamaguchi S, Oohashi T, Shimada H, Ochiai T, Yoda K, Nomura F. Overexpression and mistargeting of centromere protein-A in human primary colorectal...of cell biology. 2007;176(6):795-805. 9. Tomonaga T, Matsushita K, Yamaguchi S, Oohashi T, Shimada H, Ochiai T, Yoda K, Nomura F. Overexpression and

Development of New Approaches for Breast Cancer Therapy and Diagnosis Based on Angiogenesis

Science.gov (United States)

1997-10-01

Immune complexes were incubated for two hours at 4°C with rabbit anti-mouse IgG and then were precipitated upon addition of proteinA - Sepharose 4B...from the cloned hybridomas. These studies are just getting underway. CONCLUSIONS The main objective of this grant is identify specific molecular

AFRRI (Armed Forces Radiobiology Research Institute) Reports, October, November, December 1988

Science.gov (United States)

1988-10-01

protein. The protein concentration is estimated by assuming that an average granule contained about 0.38 pg proteina and the assay solution contained...e.g.. in conjunction with GSH peroxidase. GSH esters have been developed as a means of getting GSH into cells (Figure 6), and the monoethyl ester of

Fetal effects of epidermal growth factor deficiency induced in rats by autoantibodies against epidermal growth factor

DEFF Research Database (Denmark)

Raaberg, Lasse; Nexø, Ebba; Jørgensen, P E

1995-01-01

, the amount of surfactant protein-A was decreased, suggesting a delayed lung maturation. The offspring of EGF-immunized rats had dry and wrinkled skin. The skin was thin and the hair follicles were immature. This suggests a role for EGF in the growth and development of the skin. The liver/body weight ratio...

Revista de tesis de la Facultad de Medicina de Bogotá. Protozoología del hematozoario del paludismo en el húesped humano

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Facultad de Medicina Revista

1944-05-01

Full Text Available Protozoología del Hematozoario del paludismo en el huesped humano. Julián de Zuleta / Las proteinas del plasma en las deshidrataciones del niño. Alfonso Cárdenas Murcia / Duración del trabajo en las clínicas obstétricas del Hopsital de San Juan de Dios

Diversity of virulence genes in Brucella melitensis and Brucella abortus detected from patients with rheumatoid arthritis.

Science.gov (United States)

Rahdar, Hossein Ali; Golmohammadi, Reza; Mirnejad, Reza; Ataee, Ramezan Ali; Alishiri, Gholam Hossein; Kazemian, Hossein

2018-03-22

The presence of Brucella melitensis and Brucella abortus genomes were investigated in the synovial fluid (SF) samples from 90 patients with rheumatoid arthritis (RA). DNA extraction and PCR assay were performed for simultaneous identification and discrimination of B. melitensis and B. abortus from the SF using three specific primers. After gel electrophoresis, the PCR products were confirmed by DNA sequencing. The cbg, omp31, manA, virB, and znuA virulence genes typing were performed by multiplex-PCR. Of the 90 samples, 14 were positive for B. melitensis (n = 9; 10%) and B. abortus (n = 5; 5.5%). The virulotyping of positive samples revealed the presence of all five virulence genes in B. melitensis. The virB, cbg, and om31 were detected in all five samples of B. abortus. In addition, zhuA and manA were detected in three (60%) and four (80%) samples, respectively, of the B. abortus-positive samples. Moreover, a total of 94.2% and 89.2% of the 14 positive samples were also found positive for manA and znuA, respectively. Our findings revealed that the Brucella spp. genomes can be detected in the SF of RA patients by the PCR-based method. We thus suggest that physicians should consider the Brucella spp. as indicators of potential RA for the timely diagnosis and treatment of RA. Copyright © 2018. Published by Elsevier Ltd.

ICEApl1, an integrative conjugative element related to ICEHin1056, identified in the pig pathogen Actinobacillus pleuropneumoniae

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Janine T Bosse

2016-06-01

Full Text Available ICEApl1 was identified in the whole genome sequence of MIDG2331, a tetracycline-resistant (MIC = 8 mg/L serovar 8 clinical isolate of Actinobacillus pleuropneumoniae, the causative agent of porcine pleuropneumonia. PCR amplification of virB4, one of the core genes involved in conjugation, was used to identify other A. pleuropneumoniae isolates potentially carrying ICEApl1. MICs for tetracycline were determined for virB4 positive isolates, and shotgun whole genome sequence analysis was used to confirm presence of the complete ICEApl1. The sequence of ICEApl1 is 56083 bp long and contains 67 genes including a Tn10 element encoding tetracycline resistance. Comparative sequence analysis was performed with similar integrative conjugative elements (ICEs found in other members of the Pasteurellaceae. ICEApl1 is most similar to the 59393 bp ICEHin1056, from Haemophilus influenzae strain 1056. Although initially identified only in serovar 8 isolates of A. pleuropneumoniae (31 from the UK and 1 from Cyprus, conjugal transfer of ICEApl1 to representative isolates of other serovars was confirmed. All isolates carrying ICEApl1 had a MIC for tetracycline of 8 mg/L. This is, to our knowledge, the first description of an ICE in A. pleuropneumoniae, and the first report of a member of the ICEHin1056 subfamily in a non-human pathogen. ICEApl1 confers resistance to tetracycline, currently one of the more commonly used antibiotics for treatment and control of porcine pleuropneumonia.

Involvement of the Helicobacter pylori plasticity region and cag pathogenicity island genes in the development of gastroduodenal diseases.

Science.gov (United States)

Pacheco, A R; Proença-Módena, J L; Sales, A I L; Fukuhara, Y; da Silveira, W D; Pimenta-Módena, J L; de Oliveira, R B; Brocchi, M

2008-11-01

Infection by Helicobacter pylori is associated with the development of several gastroduodenal diseases, including gastritis, peptic ulcer disease (gastric ulcers and duodenal ulcers), and gastric adenocarcinoma. Although a number of putative virulence factors have been reported for H. pylori, there are conflicting results regarding their association with specific H. pylori-related diseases. In this work, we investigated the presence of virB11 and cagT, located in the left half of the cag pathogenicity island (cagPAI), and the jhp917-jhp918 sequences, components of the dupA gene located in the plasticity zone of H. pylori, in Brazilian isolates of H. pylori. We also examined the association between these genes and H. pylori-related gastritis, peptic ulcer disease, and gastric and duodenal ulcers in an attempt to identify a gene marker for clinical outcomes related to infection by H. pylori. The cagT gene was associated with peptic ulcer disease and gastric ulcers, whereas the virB11 gene was detected in nearly all of the samples. The dupA gene was not associated with duodenal ulcers or any gastroduodenal disease here analyzed. These results suggest that cagT could be a useful prognostic marker for the development of peptic ulcer disease in the state of São Paulo, Brazil. They also indicate that cagT is associated with greater virulence and peptic ulceration, and that this gene is an essential component of the type IV secretion system of H. pylori.

Revista de revistas

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Facultad de Medicina Revista

1946-10-01

Full Text Available The Lanced Londres, abril 6 de 1946. Necrosis aguda del hígado. Tratada con hidrolizados de proteinas. Osteomielitis que semeja radiográficamente un sarvoma. BCG en Escandinavia / The Lnaces Londres, mayo 9 de 1946. Miopía tirotóxica aguda. Posible papel de la prehipófesis en la diabetes humana

AFRRI (Armed Forces Radiobiology Research Institute) Reports, October, January-March 1989

Science.gov (United States)

1989-01-01

conservative synthesis 1,ir 301 mitn at room temperature. the MimxtUre wit’ centri- during the time allowed for repair. The photolysis tu~ get t1 2001i) g it...differeccs in rccow rv at thc cellular level. oft proteinas anrd iolher mnoleculecs to nucleic acids. In In Radiation andi Cellular C onio-ol Prmnt.ie

mRNA metabolism: nonsense mediated mRNA decay as a tool for gene therapy and the role of human DIS3L2 in transcript degradation

OpenAIRE

Amaral, Gerson Leonel Asper

2015-01-01

Tese de mestrado em Biologia Humana e Ambiente, apresentada à Universidade de Lisboa, através da Faculdade de Ciências, 2015 A expressão génica nos eucariotas envolve uma série de passos interligados e acoplados entre si, tendo a molécula de RNA (ribonucleic acid) como mensageiro entre os grandes passos. Resumidamente, a mensagem codificada pelas bases nucleotídicas do ácido desoxirribonucleico (DNA) (deoxyribonucleic acid) é transferida para uma molécula de RNA (transcrição), que, após pr...

Arquitectura y prestaciones de un router de conmutación de paquetes ópticos para las futuras redes de Internet

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Gustavo Puerto Leguizamón

2010-01-01

Full Text Available En este artículo se describe la arquitectura, funcionamiento y prestaciones de un prototipo de router de conmutación de paquetes ópticos con intercambio de etiqueta y con capacidad para procesar dos canales independientes. Además se describen los principales subsistemas usados en la arquitectura del router óptico de conmutación de paquetes en el cual se transportan 10Gb/s NRZ de carga de datos junto con 155Mb/s NRZ de etiqueta codificada en SCM sobre una subportadora de RF de 18GHz.

Gestión Institucional como Medio de Cumplimiento de la ley Anti Bullying en la Lucha contra la Violencia Escolar y Maltrato entre Iguales Institución Educativa Secundaria “Maria Auxiliadora” Puno, Año Escolar 2015

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Calsín Ramos, Patricia Yolanda

2016-01-01

En la investigación titulada:“Gestión institucional como medio de cumplimiento de la Ley Antibulling en la lucha contra la violencia escolar y maltrato entre iguales,en la institución educativa secundaria “María Auxiliadora”, Puno, año escolar 2015”,se utilizó el paradigma cuantitativo, que cuenta con una metodología hipotética - deductiva, utilizando un instrumento y técnica que es la encuesta pre-codificada, y el programa SPSS para el procesamiento estadístico de los cuadros de aná...

Factores Sociales y Jurídicos de revocatoria de Alcaldes Municipales por la Des-centralización Presupuestal Puno 2011-2013

OpenAIRE

De La Cruz Cornejo, Yonell

2015-01-01

La presente investigación lleva como título “factores sociales y jurídicos de revocatorias de alcaldes municipales por la descentralización presupuestal puno 2011-2013”; utilizo el paradigma cuantitativo, que cuenta con una metodología hipotética - deductiva, utilizando un instrumento y técnica que es la encuesta pre- codificada, y el programa SPSS para el procesamiento estadístico de los cuadros de análisis con la aplicación de la lógica deductiva en el entendimiento de l...

CsrA and Cra influence Shigella flexneri pathogenesis.

Science.gov (United States)

Gore, Aja L; Payne, Shelley M

2010-11-01

Shigella flexneri is a facultative intracellular pathogen that invades and disrupts the colonic epithelium. In order to thrive in the host, S. flexneri must adapt to environmental conditions in the gut and within the eukaryotic cytosol, including variability in the available carbon sources and other nutrients. We examined the roles of the carbon consumption regulators CsrA and Cra in a cell culture model of S. flexneri virulence. CsrA is an activator of glycolysis and a repressor of gluconeogenesis, and a csrA mutant had decreased attachment and invasion of cultured cells. Conversely, Cra represses glycolysis and activates gluconeogenesis, and the cra mutant had an increase in both attachment and invasion compared to the wild-type strain. Both mutants were defective in plaque formation. The importance of the glycolytic pathway in invasion and plaque formation was confirmed by testing the effect of a mutation in the glycolysis gene pfkA. The pfkA mutant was noninvasive and had cell surface alterations as indicated by decreased sensitivity to SDS and an altered lipopolysaccharide profile. The loss of invasion by the csrA and pfkA mutants was due to decreased expression of the S. flexneri virulence factor regulators virF and virB, resulting in decreased production of Shigella invasion plasmid antigens (Ipa). These data indicate that regulation of carbon metabolism and expression of the glycolysis gene pfkA are critical for synthesis of the virulence gene regulators VirF and VirB, and both the glycolytic and gluconeogenic pathways influence steps in S. flexneri invasion and plaque formation.

Correlation of nutritional status and food intake in hemodialysis patients Correlação entre estado nutricional e ingestão alimentar em pacientes de hemodiálise

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Alvaro A. C. Morais

2005-06-01

nutricional com ingestão alimentar, um estudo prospectivo foi realizado com pacientes ambulatoriais. MÉTODOS: Doentes estáveis sibmetidos a hemodiálise crônica por no mínimo 3 meses (n= 44 foram investigados mediante recordatório alimentar e determinações convencionais antropométricas, bioquímicas e de bioimpedância , incluindo-se avaliação global subjetiva e também objetiva. A idade do grupo era de 47.0 ± 16.9 anos com 63.6% de homens. O índice de massa corporal situava-se em 22.2 ± 3.9 kg/m2, a ingestão calórica foi de 1471 ± 601 kcal/dia (20.7 ± 6.7 kcal/kg/dia e o consumo proteico atingiu 74.3 ± 16.6 g proteina/dia (1.2 g/kg/dia . As variáveis dietéticas e clínicas foram correlacionadas com os índices nutricionais através da análise de regressão linear. RESULTADOS: A desnutrição estimada pela avalia;áo global subjetiva foi muito comum (>90%, apesar de que o índice de massa corporal e a taxa de albumina estavam aceitáveis na maioria da população. A avaliação objetiva global evidenciou resultados numericamente parecidos, com 6,8% bem nutridos, 61,4% com risco nutricional ou desnutrição leve, 29,6% no patamar moderado e 2,3% exibindo desnutrição grave. O ganho calórico total não apresentou associações, todavia ingressos de proteina, carboidratos e lípides se correlacionaram positivamente com a prega cutânea do tríceps (P=0.02. Apenas a ingestão lipídica associou-se diretamente com a circunferência do braço, demonstrando ainda correlação com o índice de massa corporal bem como com a gordura corpórea total (bioimpedância (P<0.001. CONCLUSÕES: 1 0s teores de proteinas, lípides e carboidratos na dieta exibiram um certo número de correlações com variáveis antropométricas e de bioimpedância; 2 A ingestão lipídica foi o melhor índice nesta experiência, ultrapassando a proteina ou o ganho energético total; 3 Em que pesem algumas limitações, o recordatório alimentar foi útil na avaliação destes pacientes de

The Structure and Infrastructure of Chinese Science and Technology

Science.gov (United States)

2006-01-01

Series C-Life Sciences 38 Theoretical And Applied Genetics 32 Journal Of Forensic Sciences 31 Febs Letters 31 Acta Botanica Sinica 30 Protein...A1-18 – Journals Containing Most Crops Papers JOURNAL #PAPERS Acta Botanica Sinica 233 Chinese Science Bulletin 128 Theoretical And Applied...physiological VEGETABLES; QUALITY; ACTA BOTANICA SINICA BIOLOGICAL & MEDICAL SCIENCES STRESS PHYSIOLOGY 6.3 5 2 19 16 212 To extract v Investiga

LA PROTEOMICA EN LA ERA POSTGENÓMICA

OpenAIRE

MARÍA CLAUDIA SANDOVAL-USME; ADRIANA UMAÑA- PÉREZ; ANDRÉS FELIPE VALLEJO-PULIDO; CATALINA ARÉVALO-FERRO; MYRIAM SÁNCHEZ- GÓMEZ

2009-01-01

El principal desafío de la biología moderna es entender la expresión, función y regulación del conjunto completo de proteínas codificadas por un organismo, lo cual describe el objetivo del nuevo campo de la proteómica. Las proteínas son las efectoras del trabajo celular, por ello el estudio de sus perfiles globales de expresión y de sus cambios bajo determinadas condiciones fisiológicas o patológicas, permite entender la red compleja de interacciones en que se basa el funcionamiento de una cé...

Análise do gene KISS1 nos distúrbios puberais humanos

OpenAIRE

Letícia Ferreira Gontijo Silveira

2009-01-01

A kisspeptina, codificada pelo gene KISS1, é um neuropeptídeo crucial na regulação do início da puberdade. A kisspeptina estimula a secreção hipotalâmica do hormônio liberador de gonadotrofinas (GnRH) após se ligar ao seu receptor GPR54. Mutações inativadoras do GPR54 são atualmente consideradas como uma causa rara de hipogonadismo hipogonadotrófico isolado (HHI) normósmico. Recentemente, uma mutação ativadora no receptor GPR54 foi implicada na patogênese da puberdade precoce dependente de go...

Transformación genética de olivo (Olea europaea L.) con un gen de Medicago truncatula que codifica para un proteína tipo FT

OpenAIRE

Cerezo Medina, Sergio; Barceló-Muñoz, Araceli; Samach, Alon; Mercado Carmona, José A.; Pliego Alfaro, Fernando

2013-01-01

En el olivo (Olea europaea L.), la modificación de la arquitectura de la planta y la reducción del periodo juvenil son caracteres de interés en la mejora. Las proteínas codificadas por genes tipo FT (FLOWERING LOCUS T), además de actuar como componente principal de la señal sistémica inductora de floración conocida como florígeno, participan en la regulación de otros procesos del desarrollo en plantas, entre ellos, la determinación de la arquitectura o la dormancia. El objetivo de este trabaj...

Estabilidad física y química de preparaciones líquidas extemporáneas elaboradas a partir de tabletas de captopril

OpenAIRE

Olaya, Maria del Pilar; Muñoz, Ilvar José; Ponce D´León, Luisa Fernanda; Barbosa, Helber; Bautista, Sandra Elizabeth

2008-01-01

Se estudió la estabilidad de preparaciones líquidas extemporáneas para administración peroral, elaboradas  a partir de tabletas de captopril de dos marcas comerciales del mercado nacional colombiano, codificadas como A y B, utilizando solventes de uso común en hospitales. Vehículo 1: agua destilada + vitamina C 10 %  (98 % : 2 %), Vehículo 2: dextrosa 10 % en agua destilada + vitamina C 10 % (98 % : 2 %). Las muestras fueron almacenadas a 5 ºC, 25 ºC y 40 ºC. La cuantificación del fármaco se ...

Control del crecimiento y desarrollo de las plantas por la luz: Funciones fotoperceptivas y mecanismos de acción del fitocromo A

OpenAIRE

Yanovsky, Marcelo Javier

1999-01-01

La luz es un factor ambiental crítico para el crecimiento y desarrollo de las plantas. Provee no solo la energía lumínica necesaria para el proceso fotosíntético, sino también señales informativas que las plantas utilizan para coordinar su crecimiento y desarrollo con el ambiente circundante. La percepción de las señales lumínicas esta mediada por fotorreceptores específicos como los fitocromos. Estos son una pequeña familia de proteínas codificada por cinco genes en Arabidopsis thaliana, FIT...

Iron Starvation Conditions Upregulate Ehrlichia ruminantium Type IV Secretion System, tr1 Transcription Factor and map1 Genes Family through the Master Regulatory Protein ErxR

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Amal Moumène

2018-01-01

Full Text Available Ehrlichia ruminantium is an obligatory intracellular bacterium that causes heartwater, a fatal disease in ruminants. Due to its intracellular nature, E. ruminantium requires a set of specific virulence factors, such as the type IV secretion system (T4SS, and outer membrane proteins (Map proteins in order to avoid and subvert the host's immune response. Several studies have been conducted to understand the regulation of the T4SS or outer membrane proteins, in Ehrlichia, but no integrated approach has been used to understand the regulation of Ehrlichia pathogenicity determinants in response to environmental cues. Iron is known to be a key nutrient for bacterial growth both in the environment and within hosts. In this study, we experimentally demonstrated the regulation of virB, map1, and tr1 genes by the newly identified master regulator ErxR (for Ehrlichia ruminantium expression regulator. We also analyzed the effect of iron depletion on the expression of erxR gene, tr1 transcription factor, T4SS and map1 genes clusters in E. ruminantium. We show that exposure of E. ruminantium to iron starvation induces erxR and subsequently tr1, virB, and map1 genes. Our results reveal tight co-regulation of T4SS and map1 genes via the ErxR regulatory protein at the transcriptional level, and, for the first time link map genes to the virulence function sensu stricto, thereby advancing our understanding of Ehrlichia's infection process. These results suggest that Ehrlichia is able to sense changes in iron concentrations in the environment and to regulate the expression of virulence factors accordingly.

Quantitative Expression Analysis of SpA, FnbA and Rsp Genes in Staphylococcus aureus: Actively Associated in the Formation of Biofilms.

Science.gov (United States)

Yeswanth, Sthanikam; Chaudhury, Abhijit; Sarma, Potukuchi Venkata Gurunadha Krishna

2017-12-01

In Staphylococcus aureus, adherence and secretory proteins play chief role in the formation of biofilms. This mode of growth exhibits resistance to a variety of antibiotics and spreads its infections. In the present study, secretary and adherence proteins, Protein-A, Fibronectin-binding protein-A (FnbA) and Rsp (a transcription regulator encoding proteolytic property) expression levels were evaluated at different stages of growth in S. aureus ATCC12600 a drug-sensitive strain and multidrug-resistant strains of S. aureus. Initially, the SpA, FnbA and Rsp genes of S. aureus ATCC12600 were cloned, sequenced, expressed and characterized. The proteolytic property of recombinant Rsp was conspicuously shown when this pathogen was grown in aerobic conditions correlating with reduced biofilm units. In anaerobic mode of growth, S. aureus exhibited a higher expression of SpA and FnbA in early and mid adherence phases and finally stabilized at 48 h of incubation. This expression was more pronounced in methicillin-resistant strains (LMV1-8 and D1-4) of S. aureus. In all these stages, Rsp gene expression was at the lowest level and these results concur with the increased biofilm units. The results of the present study explain proteins chiefly contribute in the formation of biofilms.

Development of multiplex polymerase chain reaction for detection of Ehrlichia canis, Babesia spp and Hepatozoon canis in canine blood.

Science.gov (United States)

Kledmanee, Kan; Suwanpakdee, Sarin; Krajangwong, Sakranmanee; Chatsiriwech, Jarin; Suksai, Parut; Suwannachat, Pongpun; Sariya, Ladawan; Buddhirongawatr, Ruangrat; Charoonrut, Phingphol; Chaichoun, Kridsada

2009-01-01

A multiplex polymerase chain reaction (PCR) has been developed for simultaneous detection of canine blood parasites, Ehrlichia canis, Babesia spp and Hepatozoon canis, from blood samples in a single reaction. The multiplex PCR primers were specific to E. canis VirB9, Babesia spp 16S rRNA and H. canis 16S rRNA genes. Specificity of the amplicons was confirmed by DNA sequencing. The assay was evaluated using normal canine and infected blood samples, which were detected by microscopic examination. This multiplex PCR offers scope for simultaneous detection of three important canine blood parasites and should be valuable in monitoring parasite infections in dogs and ticks.

Ispitivanje primene neorganskih supstrata na bazi nanokristala TiO2 za detekciju i kvantifikaciju malih molekula SALDI TOF masenom spektrometrijom

OpenAIRE

Popović, Iva A.

2017-01-01

Izvod: MALDI TOF (Matrix Assisted Laser Desorption and Ionization Time-Of-Flight) masena spektrometrija je meka jonizaciona tehnika koja se prvenstveno koristi za analizu biomolekula (proteina i nukleinskih kiselina) i omogućava detekciju molekula uz veoma nizak stepen fragmentacije. Kako bi se sprečila/ublažila fragmentacija izazvana direktnom ekscitacijom laserom, uzorak se meša sa matricama koje imaju visok apsorpcioni koeficijent u oblasti u kojoj laser emituje. Primarn...

Inhibition of Xenobiotic-Degrading Hydrolases by Organophosphinates

Science.gov (United States)

1986-07-01

M 4 Q r 000 44 Table 11. Purification of arylester hydrolase Specific Total Total Activity Volume Activity Proteina (Umoles/ Purifi- Fraction (mL...did get re-adjusted after the sample was applied. After the sample was applied the column was washed with the above MES buffer an.+eluted with 100 ml...Lieske (94) and compared them to the reversed phase HPLC retention times we have previously reported (16). We get an excellent linear correlation

INFLUENCIA DE LAS O-GLICOSILACIONES SOBRE LA VELOCIDAD DE HIDRATACION Y ESTRUCTURA DE MUCINAS RESPIRATORIAS.

OpenAIRE

ESPINOSA DE LA FUENTE, MARISOL; ESPINOSA DE LA FUENTE, MARISOL

2008-01-01

EL MOCO ES UN GEL POLIMERICO E HIDROFILICO QUE CUBRE DIVERSAS SUPERFICIES EPITELIALES Y ES PARTE DEL MECANISMO DE DEFENSA INNATO PRESENTE EN EL TRACTO RESPIRATORIO HUMANO. LAS DIVERSAS PARTICULAS INHALADAS Y AGENTES PATOGENOS QUE QUEDAN ATRAPADOS EN ESTE, SON ELIMINADOS A TRAVES DEL TRANSPORTE MUCOCILIAR ("CLEARENCE" MUCOCILIAR). EL MOCO, ESTA FORMADO POR UNA MATRIZ POLIMERICA (1 POR CIENTO), AGUA (95 POR CIENTO) Y TAMBIEN IONES Y OTRAS PROTEINAS (2 POR CIENTO). LA MATRIZ POLIMERICA...

Correspondências - arte, técnica e processo histórico

OpenAIRE

Marcos Tadeu Fabris Gonçalves

2011-01-01

O objetivo desta tese é identificar alguns dos momentos da tradição artística visual moderna que pretenderam mapear a ascensão, a consolidação e a devastação do projeto de modernização do ponto de vista de suas vítimas no âmbito de um mercado no qual a arte e a cultura se consolidam, em passo acelerado, como mercadorias. A partir das demandas para a arte moderna codificadas na obra de Charles Baudelaire e das conquistas técnicas sedimentadas na pintura de Gustave Courbet, Jean-François Millet...

Identification and analysis of novel virulence-defective Rhodococcus fascians mutants

OpenAIRE

Cabecinhas, Adriana Sofia Silva

2014-01-01

Rhodococcus fascians é uma actinomiceta fitopatogénica que induz uma doença, conhecida como irritação frondosa, caracterizada pela indução de múltiplos rebentos, numa vasta gama de plantas herbáceas dicotiledóneas. O principal factor de patogenicidade da bactéria é a produção de uma mistura de 6 citoquininas codificadas pelos genes do operão fas que está localizado num plasmídeo linear associado à virulência, pFiD188. Este trabalho teve como objectivo a análise de dois novos loci deste plasmí...

Polimorfismos del gen Butirilcolinesterasa responsables de reacciones adversas en pacientes consumidores de “cocaína”

OpenAIRE

Estrada Serrato, Carlos

2014-01-01

La butirilcolinesterasa humana (BChE; EC 3.1.1.8) es una enzima polimórfica sintetizada en el hígado y en el tejido adiposo, ampliamente distribuida en el organismo y encargada de hidrolizar algunos ésteres de colina como la procaína, ésteres alifáticos como el ácido acetilsalicílico, fármacos como la metilprednisolona, el mivacurium y la succinilcolina y drogas de uso y/o abuso como la heroína y la cocaína. Es codificada por el gen BCHE (OMIM 177400), habiéndose identificado más de 100 varia...

Cloning and transmembrane glycoprotein expression of the retrovirus HTLV-1 in mammals' cells

OpenAIRE

Penteado, Flora Cristina Lobo; Medeiros, Luciene; Orellana, Maristela Delgado; Palma, Patricia; Fontes, Aparecida Maria; Takayanagui, Osvaldo Massaiti; Covas, Dimas Tadeu

2006-01-01

O retrovírus linfotrópico de células T humanas tipo 1 é o agente etiológico da leucemia das células T do adulto e da paraparesia espástica tropical/mielopatia associada ao HTLV-1. O genoma proviral é composto por 9.032 pares de bases, contendo genes estruturais e regulatórios. A glicoproteína transmembrana gp 21 é codificada pelo gene estrutural env. O desenvolvimento de metodologias para a expressão heteróloga de proteínas, assim como a obtenção de uma linhagem celular que expresse a gp 21 r...

Purificación y caracterización electroforética de la proteína de unión a GH (GHBP en suero humano y de pacientes con cáncer y en embarazo: evidencia de la existencia de una proteína de unión a prolactina (PRLBP

Directory of Open Access Journals (Sweden)

Elizabeth Rico

2000-06-01

Full Text Available Una de las formas de regulación de la actividad de ciertas citocinas es la generación de formas solubles del receptor, las cuales actúan principalmente como proteínas de unión, aunque también se les han atribuido funciones de carácter agonista. El objetivo de este trabajo fue el de establecer la presencia de proteinas de unión a hormona de crecimiento (GHBP y prolactina (PRLBP en suero humano. La GHBP se purificó parcialmente mediante un nuevo método empleando hormona humana de crecimieiito (GH, growth hormone humana inmovilizada sobre Sepharosa y su separación y detección se realizó por medio de electroforesis e inmunotransferencia (Western blot, utilizando un anticuerpo dirigido contra el receptor de GH. Los resultados mostraron que la GHBP de suero humano es una mezcla compleja de proteínas con pesos en el rango de 27 a 62 kDa, relacionadas estructuralmente con el receptor de GH y cuyo origen está por determinarse. El suero proveniente de pacientes con cáncer de seno en estados 3 y 4, así como el de embarazo, mostró el mismo perfil de proteínas aunque sus niveles fueron en todos los casos inferiores a la normalidad, siendo los más bajos los de embarazo. En cuanto a la PRLBP, mediante anticuerpos antirreceptor, se identificó una proteina sérica de 150 kDa disociable en dos subunidades de pesos 50 y 30 kDa, consistente con lo informado por otros autores. De acuerdo con el peso establecido y a su incapacidad para unir hGH, se concluyó que no es una forma soluble del receptor y se sugiere que puede ser un anticuerpo antiidiotípico presente en suero humano. No se encontraron diferencias en el perfil ni en el contenido de la PRLBP en los tres tipos de suero analizados. Los resultados obtenidos ponen en evidencia la existencia de una proteina de unión a prolactina relacionada con el receptor de PRL y cuyo origen en humanos está por establecerse.

Emergência da múltipla resistência a antimicrobianos em Vibrio cholerae isolados de pacientes com gastroenterite no Ceará, Brasil

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Hofer Ernesto

1999-01-01

Full Text Available Das 7058 amostras de Vibrio cholerae isoladas de pacientes com suspeita de síndrome coleriforme, no período de 1991 a 1993, no Estado do Ceará, foram detectadas duas com as características de múltipla resistência aos antimicrobianos (tetraciclina, ampicilina, eritromicina, sulfametoxazol-trimetoprima e ao composto vibriostático O/129 (2,4-diamino-6,7-diisopropilpteridina. Do ponto de vista bacteriológico uma amostra foi identificada como V. cholerae sorogrupo O:1, biotipo El Tor e sorovar Inaba e a outra, caracterizada como V. cholerae sorogrupo O:22, classificada bioquimicamente no tipo II de Heiberg. Foi demonstrado que apenas na amostra do sorogrupo O:1, a multirresistência era codificada por um plasmídio, transferível por conjugação para Escherichia coli K12 e amostras sensíveis de V. cholerae O1 e não O1, numa freqüência entre 8x10-2 a 5x10-6. O plasmídio responsável pela multirresistência apresentou um peso molecular de 147 Kb, compatível com as descrições em outras partes do mundo.

USSR Report, Life Sciences, Biomedical and Behavioral Sciences, No. 38.

Science.gov (United States)

1983-07-22

However, in the last few years a few of these factories have begun to have problems. After getting all sorts of good food, the chickens are begin...ning to fail and get sick. This is an extremely pressing problem today, being studied by different specialists. I think the major problem here is...34Ispol’zovaniye Proteina Kormov Zhi- votnymi" _/Utilization of Feed Protein by Animals/, Moscow, 1979, pp 123- 141. 43. Kale, K. ,_in the book

Steroid receptor coactivators 1 and 2 mediate fetal-to-maternal signaling that initiates parturition

OpenAIRE

Gao, Lu; Rabbitt, Elizabeth H.; Condon, Jennifer C.; Renthal, Nora E.; Johnston, John M.; Mitsche, Matthew A.; Chambon, Pierre; Xu, Jianming; O’Malley, Bert W.; Mendelson, Carole R.

2015-01-01

The precise mechanisms that lead to parturition are incompletely defined. Surfactant protein-A (SP-A), which is secreted by fetal lungs into amniotic fluid (AF) near term, likely provides a signal for parturition; however, SP-A–deficient mice have only a relatively modest delay (~12 hours) in parturition, suggesting additional factors. Here, we evaluated the contribution of steroid receptor coactivators 1 and 2 (SRC-1 and SRC-2), which upregulate SP-A transcription, to the parturition process...

A T4SS Effector Targets Host Cell Alpha-Enolase Contributing to Brucella abortus Intracellular Lifestyle.

Science.gov (United States)

Marchesini, María I; Morrone Seijo, Susana M; Guaimas, Francisco F; Comerci, Diego J

2016-01-01

Brucella abortus , the causative agent of bovine brucellosis, invades and replicates within cells inside a membrane-bound compartment known as the Brucella containing vacuole (BCV). After trafficking along the endocytic and secretory pathways, BCVs mature into endoplasmic reticulum-derived compartments permissive for bacterial replication. Brucella Type IV Secretion System (VirB) is a major virulence factor essential for the biogenesis of the replicative organelle. Upon infection, Brucella uses the VirB system to translocate effector proteins from the BCV into the host cell cytoplasm. Although the functions of many translocated proteins remain unknown, some of them have been demonstrated to modulate host cell signaling pathways to favor intracellular survival and replication. BPE123 (BAB2_0123) is a B. abortus VirB-translocated effector protein recently identified by our group whose function is yet unknown. In an attempt to identify host cell proteins interacting with BPE123, a pull-down assay was performed and human alpha-enolase (ENO-1) was identified by LC/MS-MS as a potential interaction partner of BPE123. These results were confirmed by immunoprecipitation assays. In bone-marrow derived macrophages infected with B. abortus , ENO-1 associates to BCVs in a BPE123-dependent manner, indicating that interaction with translocated BPE123 is also occurring during the intracellular phase of the bacterium. Furthermore, ENO-1 depletion by siRNA impaired B. abortus intracellular replication in HeLa cells, confirming a role for α-enolase during the infection process. Indeed, ENO-1 activity levels were enhanced upon B. abortus infection of THP-1 macrophagic cells, and this activation is highly dependent on BPE123. Taken together, these results suggest that interaction between BPE123 and host cell ENO-1 contributes to the intracellular lifestyle of B. abortus .

Parallel evolution of a type IV secretion system in radiating lineages of the host-restricted bacterial pathogen Bartonella.

Science.gov (United States)

Engel, Philipp; Salzburger, Walter; Liesch, Marius; Chang, Chao-Chin; Maruyama, Soichi; Lanz, Christa; Calteau, Alexandra; Lajus, Aurélie; Médigue, Claudine; Schuster, Stephan C; Dehio, Christoph

2011-02-10

Adaptive radiation is the rapid origination of multiple species from a single ancestor as the result of concurrent adaptation to disparate environments. This fundamental evolutionary process is considered to be responsible for the genesis of a great portion of the diversity of life. Bacteria have evolved enormous biological diversity by exploiting an exceptional range of environments, yet diversification of bacteria via adaptive radiation has been documented in a few cases only and the underlying molecular mechanisms are largely unknown. Here we show a compelling example of adaptive radiation in pathogenic bacteria and reveal their genetic basis. Our evolutionary genomic analyses of the α-proteobacterial genus Bartonella uncover two parallel adaptive radiations within these host-restricted mammalian pathogens. We identify a horizontally-acquired protein secretion system, which has evolved to target specific bacterial effector proteins into host cells as the evolutionary key innovation triggering these parallel adaptive radiations. We show that the functional versatility and adaptive potential of the VirB type IV secretion system (T4SS), and thereby translocated Bartonella effector proteins (Beps), evolved in parallel in the two lineages prior to their radiations. Independent chromosomal fixation of the virB operon and consecutive rounds of lineage-specific bep gene duplications followed by their functional diversification characterize these parallel evolutionary trajectories. Whereas most Beps maintained their ancestral domain constitution, strikingly, a novel type of effector protein emerged convergently in both lineages. This resulted in similar arrays of host cell-targeted effector proteins in the two lineages of Bartonella as the basis of their independent radiation. The parallel molecular evolution of the VirB/Bep system displays a striking example of a key innovation involved in independent adaptive processes and the emergence of bacterial pathogens

Conservation of the Type IV secretion system throughout Wolbachia evolution

DEFF Research Database (Denmark)

Pichon, Samuel; Bouchon, Didier; Cordaux, Richard

2009-01-01

, encoding a T4SS were previously identified and characterized at two separate genomic loci. Using the largest data set of Wolbachia strains studied so far, we show that vir gene sequence and organization are strictly conserved among 37 Wolbachia strains inducing various phenotypes such as cytoplasmic...... incompatibility, feminization, or oogenesis in their arthropod hosts. In sharp contrast, extensive variation of genomic sequences flanking the virB8-D4 operon suggested its distinct location among Wolbachia genomes. Long term conservation of the T4SS may imply maintenance of a functional effector translocation...... system in Wolbachia, thereby suggesting the importance for the T4SS in Wolbachia biology and survival inside host cells....

The In Vivo DNA Binding Properties of Wild-Type and Mutant p53 Proteins in Mammary Cell Lines During the Course of Cell Cycle.

Science.gov (United States)

1996-08-01

that my statement of work (SOW) for the current project omitted many of the tasks that had to be carried out in order to get the lab up and running...that we knew that we could stabilize wild-type p53 in ML-1 cells along with the possibility of being able to get an excellent elutriation profile with...nuclear protein extract was immunoprecipitated with PAb421 cross-linked to ProteinA -Sepharose beads and analysed by SDS-PAGE Western blot analysis with

Process Research and Development of Antibodies as Countermeasures for C. botulinum

Science.gov (United States)

2009-02-01

ProteinA –Sepharose Fast Flow resin (Amersham Biosciences). Alternatively, culture supernatant was di- rectly loaded onto the rProtein A–Sepharose Fast Flow...interest; however the stationary point was a saddle point, so we move on the ridge in the direction to get the maximum response. The points on the...10 Ridge of steepest ascent of the reduced model for getting maximum antibody production with independent vari- ables X1, X2, X3. X4 and X5 are kept

O sujeito biotecnológico na viagem pelo reino das batatas trangênicas, porquinhos fosforecentes e almas codificadas

OpenAIRE

Hüning, Simone Maria

2008-01-01

Este estudo versa sobre a centralidade que as biotecnologias vêm assumindo na constituição e compreensão da subjetividade na contemporaneidade a partir de uma pesquisa que analisa a proliferação das biotecnologias e a intensificação de enunciados sobre as mesmas na vida cotidiana. Tomando as ferramentas conceituais oferecidas pelos trabalhos de Bruno Latour e Michel Foucault, iniciase esta tese pela análise da constituição da mesma, que é permeada por questões políticas, éticas, institucionai...

Teoría del Cambio Subjetivo: Aportes desde un Estudio Cualitativo con Profesores

Directory of Open Access Journals (Sweden)

PABLO J. CASTRO

2015-01-01

Full Text Available Se reportan los hallazgos de una investigación que reconstruye la teoría subjetiva de un grupo de docentes participantesde un diplomado de educación en valores acerca de su propio proceso de cambio. A una muestra de 12 profesores chilenosse le realizaron 35 entrevistas y se analizaron algunos documentos personales. La información se procesó medianteanálisis cualitativo descriptivo y relacional, y fue codificada utilizando el programa ATLA S.ti. Los resultados indicaronque los participantes distinguen factores externos y propios como causas de sus cambios, diferencian entre vida personaly trabajo con incipientes explicaciones al integrar ambas dimensiones, y reconocen la importancia del cambio personalen el cambio profesional.

PREHRAMBENA VRIJEDNOST KALIFORNIJSKE PASTRVE (Oncorhynchus mykiss) UZGOJENE U JADRANSKOM MORU

OpenAIRE

Janči, Tibor; Kanski, Danijel; Dulić, Marina; Marušić, Nives; Medić, Helga; Petrak, Tomislav; Vidaček, Sanja

2013-01-01

Cilj ovog rada bio je određivanje kemijskog sastava fileta svježe i hladno dimljene kalifornijske pastrve (Oncorhynchus mykiss)uzgojene u Jadranskom moru mjerenjem udjela vode, masti, proteina, soli, pepela, sastava masnih kiselina s naglaskom na eikozapentaensku (EPA) i dokozaheksaensku (DHA) masnu kiselinu te fizikalnih karakteristika mjerenjem pH i određivanjem boje. Analize su izvršene na homogeniziranom mišićju nakon odvajanja kože i kostiju. Određivanje udjela vode, pepela, ukupne količ...

Eletroforese em acetato de celulose das proteínas do líquido cefalorraqueano: valores normais

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Izelino Caldas Filho

1977-09-01

Full Text Available Foi feita eletroforese das proteinas de LCR colhido por via lombar de 19 pacientes neurologicamente normais entre 10 e 54 anos. A proteinorraquia total variou entre 10 e 28 mg% (método de Meulemans. Sete pacientes eram normais e 12 apresentavam neuroses. As médias das frações separadas pela eletroforese foram: pré-albumina 4.3%; albumina 54.8%; globulina alfa-1 5.9%; globulina alfa-2 8.3%; globulinas beta 15.5%; globulinas gama 11.2%.

Role of Seprase in Breast Cancer Invasion

Science.gov (United States)

1998-09-01

length cDNA as quickly as possible, we have arranged to get help from Dr. Barbara L. Parsons at the National Center for Toxicological Research with...ctin Nvas si~paratcd frorn free (𔃻ýI1 by get I tra - iwtvdopodWa prateolysis )f e.TtraceLlular mnatrix~ tion osmAg sa. excellulose GS colunin (Pi.erce...invadopodia. MMP-2 is secreted istry of proteinas :. in tumor invasion. Physicl Revs 73, as a soluble enzyme that can be found within the 161-45, oytoplasm

Competing risks model in screening for preeclampsia by maternal factors and biomarkers at 11-13 weeks gestation.

Science.gov (United States)

O'Gorman, Neil; Wright, David; Syngelaki, Argyro; Akolekar, Ranjit; Wright, Alan; Poon, Leona C; Nicolaides, Kypros H

2016-01-01

Preeclampsia affects approximately 3% of all pregnancies and is a major cause of maternal and perinatal morbidity and death. In the last decade, extensive research has been devoted to early screening for preeclampsia with the aim of reducing the prevalence of the disease through pharmacologic intervention in the high-risk group starting from the first trimester of pregnancy. The purpose of this study was to develop a model for preeclampsia based on maternal demographic characteristics and medical history (maternal factors) and biomarkers. The data for this study were derived from prospective screening for adverse obstetric outcomes in women who attended for their routine first hospital visit at 11-13 weeks gestation in 2 maternity hospitals in England. We screened 35,948 singleton pregnancies that included 1058 pregnancies (2.9%) that experienced preeclampsia. Bayes theorem was used to combine the a priori risk from maternal factors with various combinations of uterine artery pulsatility index, mean arterial pressure, serum pregnancy-associated plasma protein-A, and placental growth factor multiple of the median values. Five-fold cross validation was used to assess the performance of screening for preeclampsia that delivered at preeclampsia) and ≥37 weeks gestation (term-preeclampsia) by models that combined maternal factors with individual biomarkers and their combination with screening by maternal factors alone. In pregnancies that experienced preeclampsia, the values of uterine artery pulsatility index and mean arterial pressure were increased, and the values of serum pregnancy-associated plasma protein-A and placental growth factor were decreased. For all biomarkers, the deviation from normal was greater for early than late preeclampsia; therefore, the performance of screening was related inversely to the gestational age at which delivery became necessary for maternal and/or fetal indications. Combined screening by maternal factors, uterine artery pulsatility

Producción y caracterización de un anticuerpo monoclonal que reconoce el fragmento 28-30 kDa de la proteína MSP-1 de Plasmodium falciparum

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Nina M. Muñoz

2000-06-01

Full Text Available Los merozoitos del parásito Plasmodium falciparum contienen en su superficie los fragmentos producidos por el primer procesamiento proteolítico de la proteina MSP-1 (merozoite surface protein-1. Dichos fragmentos tienen pesos moleculares relativos aproximados de 83, 42, 38 y 28-30 kDa, respectivamente. En este estudio se describe la producción y caracterización de un anticuerpo monoclonal, denominado 8F4, que reconoce la proteina MSP-1 dadas las siguientes características del antigeno: 1 localización subcelular, 2 peso molecular relativo, 3 período de expresión durante el ciclo eritrocítrico, 4 tipo de asociación con la membrana plasmática del parásito y 5 presencia de epítopes compartidos con MSP-1. Adicionalmente. se estableció que 8F4 reconoce el fragmento de 28-30 kDa, producto del primer procesamiento proteolitico de MSP-1. Los análisis realizados permiten confirmar que MSP-1, 28-30 , no permanece en la superficie del merozoito desoués de ocurrida la invasión. lo cual suoiere oue hace oarte del com.o le.io de polipéptidos que es liberado por el merozoito previamente al evento de la invasión. 8F4 es el primer anticuerpo monoclonal reportado hasta el momento que reconoce específicamente este fragmento.

Teoría del Cambio Subjetivo: Aportes desde un Estudio Cualitativo con Profesores / The Theory of Subjective Change: Contributions from a Qualitative Study with Teachers / Teoria da Mudança Subjetiva: Contribuições de um Estudo Qualitativo com Professores

Directory of Open Access Journals (Sweden)

PABLO J. CASTRO

2015-10-01

Full Text Available Se reportan los hallazgos de una investigación que reconstruye la teoría subjetiva de un grupo de docentes participantes de un diplomado de educación en valores acerca de su propio proceso de cambio. A una muestra de 12 profesores chilenos se le realizaron 35 entrevistas y se analizaron algunos documentos personales. La información se procesó mediante análisis cualitativo descriptivo y relacional, y fue codificada utilizando el programa ATLAS.ti. Los resultados indicaron que los participantes distinguen factores externos y propios como causas de sus cambios, diferencian entre vida personal y trabajo con incipientes explicaciones al integrar ambas dimensiones, y reconocen la importancia del cambio personal en el cambio profesional.

Autocorrelation and cross-correlation between hCGβ and PAPP-A in repeated sampling during first trimester of pregnancy

DEFF Research Database (Denmark)

Nørgaard, Pernille; Wright, Dave; Ball, Susan

2013-01-01

Theoretically, repeated sampling of free β-human chorionic gonadotropin (hCGβ) and pregnancy associated plasma protein-A (PAPP-A) in the first trimester of pregnancy might improve performance of risk assessment of trisomy 21 (T21). To assess the performance of a screening test involving repeated...... measures of biochemical markers, correlations between markers must be estimated. The aims of this study were to calculate the autocorrelation and cross-correlation between hCGβ and PAPP-A in the first trimester of pregnancy and to investigate the possible impact of gestational age at the first sample...

La biosintesis de fenilalanina durante el desarrollo de las plantas

OpenAIRE

El-Azaz, Jorge; Ávila, Concepción; Canovas-Ramos, Francisco Miguel; De La Torre, Fernando

2016-01-01

En las plantas, la biosintesis del aminoacido fenilalanina (Phe) tiene lugar en los cloroplastos a través de una ruta conocida como ruta del prefenato, la cual se inicia a partir del producto final de la ruta del siquimato. La Phe desempeña en las plantas terrestres una función esencial como componente de las proteinas y precursor de la sintesis de fenilpropanoides, una amplia familia de metabolitos que cumplen funciones muy diversas y cuya aparición y diversificación está interrrelacionada c...

Conjugative plasmid pAW63 brings new insights into the genesis of the Bacillus anthracis virulence plasmid pXO2 and of the Bacillus thuringiensis plasmid pBT9727

Directory of Open Access Journals (Sweden)

Mahillon Jacques

2005-07-01

Full Text Available Abstract Background Bacillus cereus, Bacillus anthracis and Bacillus thuringiensis belong to the genetically close-knit Bacillus cereus sensu lato group, a family of rod-shaped Gram-positive bacteria. pAW63 is the first conjugative plasmid from the B. cereus group to be completely sequenced. Results The 71,777 bp nucleotide sequence of pAW63 reveals a modular structure, including a 42 kb tra region encoding homologs of the Type IV secretion systems components VirB11, VirB4 and VirD4, as well as homologs of Gram-positive conjugation genes from Enterococcus, Lactococcus, Listeria, Streptococcus and Staphylococcus species. It also firmly establishes the existence of a common backbone between pAW63, pXO2 from Bacillus anthracis and pBT9727 from the pathogenic Bacillus thuringiensis serovar konkukian strain 97-27. The alignment of these three plasmids highlights the presence of well conserved segments, in contrast to distinct regions of high sequence plasticity. The study of their specific differences has provided a three-point reference framework that can be exploited to formulate solid hypotheses concerning the functionalities and the molecular evolution of these three closely related plasmids. This has provided insight into the chronology of their divergence, and led to the discovery of two Type II introns on pAW63, matching copies of the mobile element IS231L in different loci of pXO2 and pBT9727, and the identification on pXO2 of a 37 kb pathogenicity island (PAI containing the anthrax capsule genes. Conclusion The complete sequence determination of pAW63 has led to a functional map of the plasmid yielding insights into its conjugative apparatus, which includes T4SS-like components, as well as its resemblance to other large plasmids of Gram-positive bacteria. Of particular interest is the extensive homology shared between pAW63 and pXO2, the second virulence plasmid of B. anthracis, as well as pBT9727 from the pathogenic strain B. thuringiensis

Brucella Rough Mutant Induce Macrophage Death via Activating IRE1α Pathway of Endoplasmic Reticulum Stress by Enhanced T4SS Secretion.

Science.gov (United States)

Li, Peng; Tian, Mingxing; Bao, Yanqing; Hu, Hai; Liu, Jiameng; Yin, Yi; Ding, Chan; Wang, Shaohui; Yu, Shengqing

2017-01-01

Brucella is a Gram-negative facultative intracellular pathogen that causes the worldwide zoonosis, known as brucellosis. Brucella virulence relies mostly on its ability to invade and replicate within phagocytic cells. The type IV secretion system (T4SS) and lipopolysaccharide are two major Brucella virulence factors. Brucella rough mutants reportedly induce the death of infected macrophages, which is T4SS dependent. However, the underlying molecular mechanism remains unclear. In this study, the T4SS secretion capacities of Brucella rough mutant and its smooth wild-type strain were comparatively investigated, by constructing the firefly luciferase fused T4SS effector, BPE123 and VceC. In addition, quantitative real-time PCR and western blotting were used to analyze the T4SS expression. The results showed that T4SS expression and secretion were enhanced significantly in the Brucella rough mutant. We also found that the activity of the T4SS virB operon promoter was notably increased in the Brucella rough mutant, which depends on quorum sensing-related regulators of VjbR upregulation. Cell infection and cell death assays revealed that deletion of vjbR in the Brucella rough mutant absolutely abolished cytotoxicity within macrophages by downregulating T4SS expression. This suggests that up-regulation of T4SS promoted by VjbR in rough mutant Δ rfbE contribute to macrophage death. In addition, we found that the Brucella rough mutant induce macrophage death via activating IRE1α pathway of endoplasmic reticulum stress. Taken together, our study provide evidence that in comparison to the Brucella smooth wild-type strain, VjbR upregulation in the Brucella rough mutant increases transcription of the virB operon, resulting in overexpression of the T4SS gene, accompanied by the over-secretion of effecter proteins, thereby causing the death of infected macrophages via activating IRE1α pathway of endoplasmic reticulum stress, suggesting novel insights into the molecular

Brucella Rough Mutant Induce Macrophage Death via Activating IRE1α Pathway of Endoplasmic Reticulum Stress by Enhanced T4SS Secretion

Directory of Open Access Journals (Sweden)

Peng Li

2017-09-01

Full Text Available Brucella is a Gram-negative facultative intracellular pathogen that causes the worldwide zoonosis, known as brucellosis. Brucella virulence relies mostly on its ability to invade and replicate within phagocytic cells. The type IV secretion system (T4SS and lipopolysaccharide are two major Brucella virulence factors. Brucella rough mutants reportedly induce the death of infected macrophages, which is T4SS dependent. However, the underlying molecular mechanism remains unclear. In this study, the T4SS secretion capacities of Brucella rough mutant and its smooth wild-type strain were comparatively investigated, by constructing the firefly luciferase fused T4SS effector, BPE123 and VceC. In addition, quantitative real-time PCR and western blotting were used to analyze the T4SS expression. The results showed that T4SS expression and secretion were enhanced significantly in the Brucella rough mutant. We also found that the activity of the T4SS virB operon promoter was notably increased in the Brucella rough mutant, which depends on quorum sensing-related regulators of VjbR upregulation. Cell infection and cell death assays revealed that deletion of vjbR in the Brucella rough mutant absolutely abolished cytotoxicity within macrophages by downregulating T4SS expression. This suggests that up-regulation of T4SS promoted by VjbR in rough mutant ΔrfbE contribute to macrophage death. In addition, we found that the Brucella rough mutant induce macrophage death via activating IRE1α pathway of endoplasmic reticulum stress. Taken together, our study provide evidence that in comparison to the Brucella smooth wild-type strain, VjbR upregulation in the Brucella rough mutant increases transcription of the virB operon, resulting in overexpression of the T4SS gene, accompanied by the over-secretion of effecter proteins, thereby causing the death of infected macrophages via activating IRE1α pathway of endoplasmic reticulum stress, suggesting novel insights into the

Parallel Evolution of a Type IV Secretion System in Radiating Lineages of the Host-Restricted Bacterial Pathogen Bartonella

Science.gov (United States)

Engel, Philipp; Salzburger, Walter; Liesch, Marius; Chang, Chao-Chin; Maruyama, Soichi; Lanz, Christa; Calteau, Alexandra; Lajus, Aurélie; Médigue, Claudine; Schuster, Stephan C.; Dehio, Christoph

2011-01-01

Adaptive radiation is the rapid origination of multiple species from a single ancestor as the result of concurrent adaptation to disparate environments. This fundamental evolutionary process is considered to be responsible for the genesis of a great portion of the diversity of life. Bacteria have evolved enormous biological diversity by exploiting an exceptional range of environments, yet diversification of bacteria via adaptive radiation has been documented in a few cases only and the underlying molecular mechanisms are largely unknown. Here we show a compelling example of adaptive radiation in pathogenic bacteria and reveal their genetic basis. Our evolutionary genomic analyses of the α-proteobacterial genus Bartonella uncover two parallel adaptive radiations within these host-restricted mammalian pathogens. We identify a horizontally-acquired protein secretion system, which has evolved to target specific bacterial effector proteins into host cells as the evolutionary key innovation triggering these parallel adaptive radiations. We show that the functional versatility and adaptive potential of the VirB type IV secretion system (T4SS), and thereby translocated Bartonella effector proteins (Beps), evolved in parallel in the two lineages prior to their radiations. Independent chromosomal fixation of the virB operon and consecutive rounds of lineage-specific bep gene duplications followed by their functional diversification characterize these parallel evolutionary trajectories. Whereas most Beps maintained their ancestral domain constitution, strikingly, a novel type of effector protein emerged convergently in both lineages. This resulted in similar arrays of host cell-targeted effector proteins in the two lineages of Bartonella as the basis of their independent radiation. The parallel molecular evolution of the VirB/Bep system displays a striking example of a key innovation involved in independent adaptive processes and the emergence of bacterial pathogens

Prevalence and characterization of Campylobacter jejuni isolated from pasture flock poultry.

Science.gov (United States)

Hanning, Irene; Biswas, Debabrata; Herrera, Paul; Roesler, Mary; Ricke, Steven C

2010-09-01

The growing interest in organic and natural foods warrants a greater need for information on the food safety of these products. In this study, samples were taken from 2 pasture flock farms (N = 178; feed, water, drag swabs, and insect traps), pasture flock retail carcasses (N = 48) and 1 pasture flock processing facility (N = 16) over a period of 8 mo. A total of 105 Campylobacter isolates were obtained from 53 (30%), 36 (75%), and 16 (100%) samples from the farms, retail carcasses, and processing facility, respectively. Of the 105 isolates collected, 65 were C. jejuni, 31 were C. coli, and 9 were other Campylobacter spp. Using PCR, the C. jejuni isolates were further analyzed for virulence genes involved in colonization and survival (flaA, flaC, cadF, dnaJ, racR, cbrR), invasion (virB11, ciaB, pldA), protection against harsh conditions (sodB, htrA, clpA), toxin production (cdtA, cdtB, cdtC), siderophore transport (ceuE), and ganglioside mimicry (wlaN). In addition, the short variable region of the flaA locus (flaA SVR) was sequenced to determine the genetic diversity of the C. jejuni isolates. The flaA SVR diversity indices increased along the farm to carcass continuum. PCR-based analysis indicated a low prevalence of 5 genes involved in colonization (dnaJ, ciaB, pldA, racR, virB11). The results of this survey indicate that the prevalence of Campylobacter on organic retail carcasses is similar to prevalence reports of Campylobacter on conventional retail carcasses. However, the genetic diversity of the flaA SVR genotypes increased along the farm to carcass continuum that contrasted with conventional poultry studies. Campylobacter jejuni is a leading cause of foodborne illness with poultry and poultry products being leading sources of infection. Free-range and pasture flock chickens are becoming more popular; however, there is an inherent biosecurity risk that can increase the prevalence of foodborne pathogens in these flocks. This study aimed to determine sources

Parallel evolution of a type IV secretion system in radiating lineages of the host-restricted bacterial pathogen Bartonella.

Directory of Open Access Journals (Sweden)

Philipp Engel

2011-02-01

Full Text Available Adaptive radiation is the rapid origination of multiple species from a single ancestor as the result of concurrent adaptation to disparate environments. This fundamental evolutionary process is considered to be responsible for the genesis of a great portion of the diversity of life. Bacteria have evolved enormous biological diversity by exploiting an exceptional range of environments, yet diversification of bacteria via adaptive radiation has been documented in a few cases only and the underlying molecular mechanisms are largely unknown. Here we show a compelling example of adaptive radiation in pathogenic bacteria and reveal their genetic basis. Our evolutionary genomic analyses of the α-proteobacterial genus Bartonella uncover two parallel adaptive radiations within these host-restricted mammalian pathogens. We identify a horizontally-acquired protein secretion system, which has evolved to target specific bacterial effector proteins into host cells as the evolutionary key innovation triggering these parallel adaptive radiations. We show that the functional versatility and adaptive potential of the VirB type IV secretion system (T4SS, and thereby translocated Bartonella effector proteins (Beps, evolved in parallel in the two lineages prior to their radiations. Independent chromosomal fixation of the virB operon and consecutive rounds of lineage-specific bep gene duplications followed by their functional diversification characterize these parallel evolutionary trajectories. Whereas most Beps maintained their ancestral domain constitution, strikingly, a novel type of effector protein emerged convergently in both lineages. This resulted in similar arrays of host cell-targeted effector proteins in the two lineages of Bartonella as the basis of their independent radiation. The parallel molecular evolution of the VirB/Bep system displays a striking example of a key innovation involved in independent adaptive processes and the emergence of bacterial

Evidencia de orígenes filogenéticos diferentes de dos aislamientos mexicanos del virus del mosaico de la caña de azúcar (SCMV

Directory of Open Access Journals (Sweden)

Ortiz Rojas Luz Yineth

2012-03-01

Full Text Available

El análisis molecular del cistron, (ue codica para la proteina de la cubierta del virus del mosaico de la caña de azúcar (SCMV reportado en la base de datos del banco de genes (GenBank, reveló la pre- sencia de 65 nucleotidos adicionales (ue codican para (uince aminoãcidos, en la region amino de la secuencia de la proteina de la cubierta del aislamiento mexicano identicado con el nümero de acceso GU474635. El análisis BLAST indicó que esta característica particular también está presente en el aislamiento DDDF6F, reportado en 1FF1 en Estados Unidos. El anãlisis logenHtico de 1I5  secuen- cias de la proteína de la cubierta de SCMV reportadas de Asia, África, Brasil y Argentina, entro otros, sugiere diJerentes origenes logeogrãcos de los aislamientos mexicanos. El aislamiento mexicano GU6N6OP5 es filogeneticamente mãs cercano a aislamientos de QCWV de Brasil X de EE.UU., mien- tras que secuencias de la proteína de la cubierta del virus SCMV reportadas en China y Alemania son

Functional properties of PSE (Pale, Soft, Exudative broiler meat in the production of mortadella

Directory of Open Access Journals (Sweden)

Cassiana Kissel

2009-11-01

Full Text Available This work was carried out in order to evaluate whether the functional properties of broiler meat are affected by the factors that lead to PSE (Pale, Soft, Exudative. PSE meat was characterized by pH and L* values, and mortadella formulations consisted of isolated soy protein, sodium tripolyphosphate, and cassava starch in addition to PSE and normal meats. The functionality of the meat was evaluated by examining the water holding capacity (WHC, texture profile, emulsion stability (ES and color of the final products. The results show that in mortadella prepared with PSE meat, the protein denaturation affected the ES. Additives are necessary to enhance the functional properties of PSE meat.Este trabalho foi realizado para avaliar o efeito dos fatores que conduzem à formação das carnes PSE (Pale, Soft, Exudative sobre as propriedades funcionais da carne de frango. Carnes PSE foram caracterizadas pelos valores de pH e L* e as formulações da mortadela consistiram de proteina isolada de soja, trifosfato de sódio, amido de mandioca e a adição das carnes normal e PSE. A funcionalidade da carne foi avaliada medindo a capacidade de retenção de água (CRA, perfil de textura, estabilidade de emulsão (EE, e cor dos produtos finais. Os resultados mostraram que na mortadela preparada com carnes PSE, as proteinas desnaturadas afetaram a EE. Aditivos são necessários para potencializar as propriedades funcionais da carne PSE.

consistencia en dome: un caso de estudio

Directory of Open Access Journals (Sweden)

DANIEL CABARCAS

2006-01-01

Full Text Available Las herramientas metaCASE ofrecen una funcionalidad similar a la de las herramientas CASE convencionales para notaciones gráficas arbitrarias, una vez estas notaciones le sean especificadas adecuadamente. La principal dificultad de dichas herramientas es la especificación de las diferentes reglas de consistencia, que deben tenerse en cuenta cuando se usa una notación. En este artículo se presenta la especificación de dos reglas de consistencia del diagrama de clases de UML en el metaCASE DOME, codificadas en el lenguaje de programación Alter. Adicionalmente, se hace un análisis comparativo entre las especificaciones de los aspectos estructurales y de las reglas de consistencia en DOME y en la especificación de UML provista por el OMG.

Identification of antigenic proteins of setaria cervi by immunoblotting technique

International Nuclear Information System (INIS)

Kaushal, N.A.; Kaushal, D.C.; Ghatak, S.

1987-01-01

Identification and characterization of antigenic proteins of Setaria cervi (bovine filarial parasite) adults and microfilariae was done by immunoblotting technique using hyperimmune rabbit sera against S. cervi and Brugia malayi. The antigens recognized by these sera were detected by using 125 I protein-A followed by autoradiography. Fifteen different antigens were observed to be common between adult and microfilarial stages of the parasite. Some stage specific antigens were also identified. Many antigens of S. cervi adults and microfilariae were also recognized by rabbit anti-B.malayi serum showing the existence of common antigenic determinants between the bovine and human filarial parasites

Alergia a proteína de leche de vaca en el menor de un año

OpenAIRE

Miquel E, Isabel; Arancibia S, M. Eugenia

2012-01-01

Se define alergia alimentaria como una reacción adversa que se produce frente a la exposición de un alimento, mediada por una respuesta inmune específica y reproducible¹. La alergia alimentaria puede ser mediada por anticuerpos de tipo IgE, por mecanismos mixtos o no mediada por IgE. Durante el primer año de vida la alergia a la proteina de leche de vaca (APLV) es la forma más frecuente de presentación de la alergia alimentaria². Esta revisión pretende entregar una propuesta al enfrentamiento...

A re-assessment of biochemical marker distributions in T21 affected and unaffected twin pregnancies in the first trimester

DEFF Research Database (Denmark)

Madsen, Helen Nordahl; Ball, Susan; Wright, Dave

2011-01-01

OBJECTIVE: To estimate the difference between levels of the two biochemical markers pregnancy-associated plasma protein-A (PAPP-A) and maternal serum free β-human chorionic gonadotropin (free β-hCG) in twin pregnancies relative to singleton pregnancies and establish an improved screening procedure...... of the biochemical markers were calculated. Detection rates and false-positive rates were estimated for screening tests incorporating nuchal translucency and maternal age, with and without biochemistry. RESULTS: Medians for the two biochemical markers for monochorionic and dichorionic twins in unaffected pregnancies......-trimester screening for chromosomal abnormalities in twins to a level comparable with that in singleton pregnancies....

39 CFR 7.7 - Enforcement.

Science.gov (United States)

2010-07-01

... 39 Postal Service 1 2010-07-01 2010-07-01 false Enforcement. 7.7 Section 7.7 Postal Service UNITED STATES POSTAL SERVICE THE BOARD OF GOVERNORS OF THE U.S. POSTAL SERVICE PUBLIC OBSERVATION (ARTICLE VII) § 7.7 Enforcement. (a) Under 5 U.S.C. 552b(g), any person may bring a proceeding in the United States...

Efficient hardware implementation of a full COFDM processor with robust channel equalization and reduced power consumption

Directory of Open Access Journals (Sweden)

Alexander López Parrado

2013-01-01

Full Text Available Este trabajo presenta el diseño de un procesador banda-base para multiplexación por división de frecuencias ortogonales codificada (COFDM de 12 Mb/s para el estándar IEEE 802.11a. El procesador COFDM banda- base fue diseñado usando circuitos diseñados por nosotros para corrección de fase de portadora, sincronización de tiempo de símbolo, ecualización de canal robusta y decodificación Viterbi. Estos circuitos son flexibles, parametrizados y descritos usando VHDL estructural y genérico. El procesador COFDM banda- base tiene dos dominios de reloj para reducción del consumo de potencia, fue sintetizado sobre un FPGA Stratix II y fue probado experimentalmente usando circuitería de radio frecuencia (RF a 2.4 GHz.

Uso de wikis como herramienta de trabajo colaborativo en un proceso de formación inicial docente

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Ilich Silva-Peña

2013-01-01

Full Text Available A través del presente artículo queremos compartir el desarrollo de una experiencia de utilización de wikis como estrategia de trabajo colaborativo en un curso de formación inicial docente. La experiencia se enmarcó en el contexto de un proyecto de Investigación+Desarrollo donde se exploraron mejoras tecnológicas a incorporar en la formación docente. Se utilizó el software Moodle ®, con características Open source. Para sistematizar los resultados desde la mirada de los/as estudiantes se aplicaron cuestionarios con respuestas abiertas, que fueron codificadas utilizando una metodología cualitativa. Los resultados muestran una alta valoración de los wikis como herramienta de aprendizaje, especialmente por el apoyo que otorga al trabajo pedagógico no presencial y asincrónico.

ACOPLAMIENTO EXCITATORIO E INHIBITORIO DE NEURONAS PULSANTES ACOPLADAS

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Francis Armando Segovia Chaves

2012-05-01

Full Text Available La información es representada como patrones de actividad neuronal o pulsos, lo que crea una diferencia significante entre las redes neuronales pulsantes y las redes neuronales clásicas. Una característica diferente de las redes neuronales pulsantes es que la información es codificada en patrones de actividad neuronal y estas se comunican usando trenes de pulsos en lugar de valores individuales. Además, este tipo de redes neuronales pulsantes trabajan con una gran cantidad de neuronas donde se requiere grandes recursos computacionales para ser simuladas. En el presente trabajo se realiza un análisis teórico de las redes neuronales pulsantes,  para el caso de dos neuronas acopladas. Se logra obtener teóricamente las condiciones para acoplamiento excitatorio e inhibitorio en las neuronas.

Association of maternal serum PAPP-A levels, nuchal translucency and crown-rump length in first trimester with adverse pregnancy outcomes: retrospective cohort study.

Science.gov (United States)

Bilagi, Ashwini; Burke, Danielle L; Riley, Richard D; Mills, Ian; Kilby, Mark D; Katie Morris, R

2017-07-01

Are first trimester serum pregnancy-associated plasma protein-A (PAPP-A), nuchal translucency (NT) and crown-rump length (CRL) prognostic factors for adverse pregnancy outcomes? Retrospective cohort, women, singleton pregnancies (UK 2011-2015). Unadjusted and multivariable logistic regression. small for gestational age (SGA), pre-eclampsia (PE), preterm birth (PTB), miscarriage, stillbirth, perinatal mortality and neonatal death (NND). A total of 12 592 pregnancies: 852 (6.8%) PTB, 352 (2.8%) PE, 1824 (14.5%) SGA, 73 (0.6%) miscarriages, 37(0.3%) stillbirths, 73 perinatal deaths (0.6%) and 38 (0.30%) NND. Multivariable analysis: lower odds of SGA [adjusted odds ratio (aOR) 0.88 (95% CI 0.85,0.91)], PTB [0.92 (95%CI 0.88,0.97)], PE [0.91 (95% CI 0.85,0.97)] and stillbirth [0.71 (95% CI 0.52,0.98)] as PAPP-A increases. Lower odds of SGA [aOR 0.79 (95% CI 0.70,0.89)] but higher odds of miscarriage [aOR 1.75 95% CI (1.12,2.72)] as NT increases, and lower odds of stillbirth as CRL increases [aOR 0.94 95% CI (0.89,0.99)]. Multivariable analysis of three factors together demonstrated strong associations: a) PAPP-A, NT, CRL and SGA, b) PAPP-A and PTB, c) PAPP-A, CRL and PE, d) NT and miscarriage. Pregnancy-associated plasma protein-A, NT and CRL are independent prognostic factors for adverse pregnancy outcomes, particularly PAPP-A and SGA with lower PAPP-A associated with increased risk. © 2017 John Wiley & Sons, Ltd. © 2017 John Wiley & Sons, Ltd.

Nuchal translucency measurement, free β-hCG and PAPP-A concentrations in IVF/ICSI pregnancies: systematic review and meta-analysis.

Science.gov (United States)

Cavoretto, Paolo; Giorgione, Veronica; Cipriani, Sonia; Viganò, Paola; Candiani, Massimo; Inversetti, Annalisa; Ricci, Elena; Parazzini, Fabio

2017-06-01

So far, data on the effect of assisted reproductive technologies (ART) on the components of first trimester combined screening for Down syndrome are still controversial. A systematic search of the literature was performed in order to identify the effect of ART, particularly in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) with fresh embryo transfer, on the nuchal translucency, free beta-human chorionic gonadotrophin and pregnancy-associated plasma protein-A measurements. Moreover, a meta-analysis and a descriptive graphical representation of the ratios between ART and spontaneous pregnancies (controls) values of median of the multiple of median (m 0 MoM) were performed. Free beta-human chorionic gonadotrophin test showed slightly higher values in the ICSI group than controls (RR = 1.09, 95%CI: 1.03-1.16) but not in the IVF group (RR = 1.03, 95%CI: 0.94-1.12). Pregnancy-associated plasma protein-A values for IVF/ICSI, IVF and ICSI showed lower values in comparison with controls (RR, 95%CI 0.85, 0.80-0.90; 0.82, 0.74-0.89 and 0.83, 0.79-0.86, respectively). The nuchal translucency measurement did not show any statistical differences between study groups (IVF and ICSI) and controls (RR = 1.00, 95%CI: 0.94-1.08 and RR = 1.01, 95%CI: 0.97-1.05, respectively). These results may be due to alterations in the placentation of ART pregnancies. Differentiating further subgroups of ART pregnancies may explain the differences in biomarker concentrations, in prenatal behavior and in obstetric outcomes between ART and spontaneous pregnancies. © 2017 John Wiley & Sons, Ltd. © 2017 John Wiley & Sons, Ltd.

7 CFR 7.1 - Administration.

Science.gov (United States)

2010-01-01

... 7 Agriculture 1 2010-01-01 2010-01-01 false Administration. 7.1 Section 7.1 Agriculture Office of the Secretary of Agriculture SELECTION AND FUNCTIONS OF AGRICULTURAL STABILIZATION AND CONSERVATION STATE, COUNTY AND COMMUNITY COMMITTEES § 7.1 Administration. (a) The regulations of this part are...

7 CFR 1221.7 - Customs.

Science.gov (United States)

2010-01-01

... 7 Agriculture 10 2010-01-01 2010-01-01 false Customs. 1221.7 Section 1221.7 Agriculture... INFORMATION ORDER Sorghum Promotion, Research, and Information Order Definitions § 1221.7 Customs. Customs means the U.S. Customs and Border Protection of the U.S. Department of Homeland Security. ...

30 CFR 7.7 - Quality assurance.

Science.gov (United States)

2010-07-01

... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Quality assurance. 7.7 Section 7.7 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR TESTING, EVALUATION, AND APPROVAL OF MINING PRODUCTS TESTING BY APPLICANT OR THIRD PARTY General § 7.7 Quality assurance. Applicants granted...

7 CFR 502.7 - Gambling.

Science.gov (United States)

2010-01-01

... 7 Agriculture 6 2010-01-01 2010-01-01 false Gambling. 502.7 Section 502.7 Agriculture Regulations... CONDUCT ON BELTSVILLE AGRICULTURE RESEARCH CENTER PROPERTY, BELTSVILLE, MARYLAND § 502.7 Gambling. Participating in games for money or other personal property, or the operation of gambling devices, the conduct...

7 CFR 2812.7 - Costs.

Science.gov (United States)

2010-01-01

... 7 Agriculture 15 2010-01-01 2010-01-01 false Costs. 2812.7 Section 2812.7 Agriculture Regulations...) § 2812.7 Costs. Donated excess personal property/equipment is free of charge. However, the donee must pay all costs associated with packaging and transportation, unless the sponsoring agency has made other...

7 CFR 503.7 - Gambling.

Science.gov (United States)

2010-01-01

... 7 Agriculture 6 2010-01-01 2010-01-01 false Gambling. 503.7 Section 503.7 Agriculture Regulations... CONDUCT ON PLUM ISLAND ANIMAL DISEASE CENTER § 503.7 Gambling. Participating in games for money or other personal property, or the operation of gambling devices, the conduct of a lottery or pool, or the selling...

7 CFR 30.7 - Group.

Science.gov (United States)

2010-01-01

... 7 Agriculture 2 2010-01-01 2010-01-01 false Group. 30.7 Section 30.7 Agriculture Regulations of... AND STANDARDS Classification of Leaf Tobacco Covering Classes, Types and Groups of Grades § 30.7 Group. A group of grades, or a division of a type covering several closely related grades, based on the...

Caracterização da interação de inositol hexafosfato e cloreto com hemoglobina humana: aspectos energéticos e estruturais

OpenAIRE

Silva, Vanessa de Cássia Teixeira da [UNESP

2010-01-01

A ligação do O2 à Hemoglobina (Hb) é um processo regulado por interações alostéricas. Os íons Inositol hexafosfato (IHP) e cloreto, e prótons, são efetores alostéricos que estabilizam a Hb na estrutura quaternária T, diminuindo a afinidade da proteina pelo O2 em meio alcalino. A ligação de IHP também altera a cooperatividade de algumas hemoglobinas como, por exemplo a hemoglobina desArg, uma variante da HbAo da qual o resíduo arginina 141 das cadeia alfas foram retirados enzimaticamente. Esta...

Interaction of calreticulin with CD40 ligand, TRAIL and Fas ligand

DEFF Research Database (Denmark)

Duus, K; Pagh, R T; Holmskov, U

2007-01-01

is utilized by many other functionally diverse molecules and in this work the interaction of calreticulin with C1q and structurally similar molecules was investigated. In addition to C1q and MBL, CD40 ligand (CD40L), tumour necrosis factor-related apoptosis-inducing ligand (TRAIL) and Fas ligand (FasL) were...... found to bind calreticulin strongly. A low level or no binding was observed for adiponectin, tumour necrosis factor-alpha (TNF-alpha), CD30L, surfactant protein-A and -D and collagen VIII. The interaction with calreticulin required a conformational change in CD40L, TRAIL and FasL and showed the same...

KlHEM13, un gen hipóxico en la biosíntesis de hemo

OpenAIRE

Blanco, Moisés

2011-01-01

[Resumen] El hemo es una molécula de interés desde el punto de vista del metabolismo energético, puesto que además de intervenir en la captura y el transporte del oxígeno (indispensable para el proceso de obtención de energía vía respiración) tambien forma parte como elemento funcional de las proteinas de la cadena de transporte electrónico y fosforilación oxidativa mitocondrial. Además, en el caso de las levaduras, actúa como un sensor de oxígeno a escala celular e interviene en la r...

7 CFR 1436.7 - Loan term.

Science.gov (United States)

2010-01-01

... 7 Agriculture 10 2010-01-01 2010-01-01 false Loan term. 1436.7 Section 1436.7 Agriculture... AGRICULTURE LOANS, PURCHASES, AND OTHER OPERATIONS FARM STORAGE FACILITY LOAN PROGRAM REGULATIONS § 1436.7 Loan term. (a) For eligible facility loan commodities other than sugar, the term of the loan will be 7...

Detection of GAD-Ab index in diabetic patients using 35S labeled recombinant human GAD65 antigen

International Nuclear Information System (INIS)

Huang Gan; Zhao Zhiguang; Peng Jian; Yan Xiang; Zhu Xuping; Yang Lin; Li Xia; Wang Jianping; Jiang Tiejian

2003-01-01

Objective: To establish a novel method for measuring glutamic acid decarboxylase autoanti-bodies(GAD-Ab). Methods: Recombinant human GAD 65 was used as the antigen, in vitro transcribed and translated 35 S-GAD 65 as the tracer, a self-designed rotating incubation apparatus as the incubator, protein-A sepharose as the precipitator, and the liquid scintillation counter was used to measure radioactive count value to detect GAD-Ab. The positive cut-off point of GAD-Ab index was determined as > 0.05 by the 99.5% percentile in 109 healthy individuals. GAD-Ab levels were determined in 43 type 1 and 226 type 2 diabetic patients. Results: The optimized working conditions included SJ1515 35 S-methionine for in vitro transcription and translation, 20-30 r/min setup of rotating incubation apparatus, test temperature 4-25 degree C, freshly prepared buffer of pH 7.2-7.4, and horizontal rotor centrifuge. The new method was better than original one, with intra-assay CV of 4.9%-8.3% and inter-assay CV of 7.1%-10.8 %, specificity of 98.2%. The results were comparable with the figures issued by an international standardized laboratory (concordance was 98.3%, Kappa value 0.971). The positive rate of GAD-Ab was 58.1% (25 of 43) in type 1 and 10.2%(23 of 226) in type 2 diabetes patients, but only 1.8% (2 of 109) in healthy individuals. Conclusion: The new assay for GAD-Ab is a highly sensitive, accurate, specific and reproducible method for clinical use

7 CFR 295.7 - Indexes.

Science.gov (United States)

2010-01-01

... 7 Agriculture 4 2010-01-01 2010-01-01 false Indexes. 295.7 Section 295.7 Agriculture Regulations of the Department of Agriculture (Continued) FOOD AND NUTRITION SERVICE, DEPARTMENT OF AGRICULTURE... Officer, Information Technology Division, Food and Nutrition Service, USDA, 3101 Park Center Drive...

7 CFR 1215.7 - Fiscal year.

Science.gov (United States)

2010-01-01

... 7 Agriculture 10 2010-01-01 2010-01-01 false Fiscal year. 1215.7 Section 1215.7 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS... CONSUMER INFORMATION Popcorn Promotion, Research, and Consumer Information Order Definitions § 1215.7...

7 CFR 2903.7 - Project objectives.

Science.gov (United States)

2010-01-01

... AGRICULTURE BIODIESEL FUEL EDUCATION PROGRAM Program Description § 2903.7 Project objectives. (a) Successful... 7 Agriculture 15 2010-01-01 2010-01-01 false Project objectives. 2903.7 Section 2903.7 Agriculture... following objectives: (1) Enhance current efforts to collect and disseminate biodiesel information; (2...

7 CFR 984.7 - Marketing year.

Science.gov (United States)

2010-01-01

... 7 Agriculture 8 2010-01-01 2010-01-01 false Marketing year. 984.7 Section 984.7 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements... Regulating Handling Definitions § 984.7 Marketing year. Marketing year means the twelve months from September...

7 CFR 274.7 - Coupon management.

Science.gov (United States)

2010-01-01

... 7 Agriculture 4 2010-01-01 2010-01-01 false Coupon management. 274.7 Section 274.7 Agriculture... FOOD STAMP AND FOOD DISTRIBUTION PROGRAM ISSUANCE AND USE OF COUPONS § 274.7 Coupon management. (a) Coupon inventory management. State agencies shall establish coupon inventory management systems which...

7 CFR 983.7 - Certified pistachios.

Science.gov (United States)

2010-01-01

... 7 Agriculture 8 2010-01-01 2010-01-01 false Certified pistachios. 983.7 Section 983.7 Agriculture... and Orders; Fruits, Vegetables, Nuts), DEPARTMENT OF AGRICULTURE PISTACHIOS GROWN IN CALIFORNIA, ARIZONA, AND NEW MEXICO Definitions § 983.7 Certified pistachios. Certified pistachios are those that meet...

7 CFR 1230.7 - Customs Service.

Science.gov (United States)

2010-01-01

... 7 Agriculture 10 2010-01-01 2010-01-01 false Customs Service. 1230.7 Section 1230.7 Agriculture... CONSUMER INFORMATION Pork Promotion, Research, and Consumer Information Order Definitions § 1230.7 Customs Service. Customs Service means the United States Customs Service of the United States Department of...

7 CFR 981.7 - Edible kernel.

Science.gov (United States)

2010-01-01

... 7 Agriculture 8 2010-01-01 2010-01-01 false Edible kernel. 981.7 Section 981.7 Agriculture... Regulating Handling Definitions § 981.7 Edible kernel. Edible kernel means a kernel, piece, or particle of almond kernel that is not inedible. [41 FR 26852, June 30, 1976] ...

7 CFR 371.7 - Animal Care.

Science.gov (United States)

2010-01-01

... 7 Agriculture 5 2010-01-01 2010-01-01 false Animal Care. 371.7 Section 371.7 Agriculture Regulations of the Department of Agriculture (Continued) ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE ORGANIZATION, FUNCTIONS, AND DELEGATIONS OF AUTHORITY § 371.7 Animal Care. (a...

7 CFR 7.17 - Dual office.

Science.gov (United States)

2010-01-01

... 7 Agriculture 1 2010-01-01 2010-01-01 false Dual office. 7.17 Section 7.17 Agriculture Office of... STATE, COUNTY AND COMMUNITY COMMITTEES § 7.17 Dual office. (a) County committee membership. A member of... any other county office employee. (b) Community committee membership. A member of the community...

7 CFR 277.7 - Cash depositories.

Science.gov (United States)

2010-01-01

... 7 Agriculture 4 2010-01-01 2010-01-01 false Cash depositories. 277.7 Section 277.7 Agriculture... FOOD STAMP AND FOOD DISTRIBUTION PROGRAM PAYMENTS OF CERTAIN ADMINISTRATIVE COSTS OF STATE AGENCIES § 277.7 Cash depositories. (a) The term “cash depositories” refers to banks or other institutions which...

7 CFR 993.7 - French prunes.

Science.gov (United States)

2010-01-01

... 7 Agriculture 8 2010-01-01 2010-01-01 false French prunes. 993.7 Section 993.7 Agriculture... Order Regulating Handling Definitions § 993.7 French prunes. French prunes means: (a) Prunes produced from plums of the following varieties of plums: French (Prune d'Agen, Petite Prune d'Agen), Coates (Cox...

Molecular and Cytological Comparisons of Chromosomes 7el₁, 7el₂, 7E(e), and 7E ⁱ Derived from Thinopyrum.

Science.gov (United States)

Guo, Jun; He, Fang; Cai, Jin-Jin; Wang, Hong-Wei; Li, An-Fei; Wang, Hong-Gang; Kong, Ling-Rang

2015-01-01

Thinopyrum chromosomes 7el1, 7el2, 7E(e), and 7E(i), homoeologous to group 7 chromosomes of common wheat (Triticum aestivum), were determined to have many useful agronomical traits for wheat improvement. To analyze the genetic relationships among the 4 Thinopyrum 7E chromosomes, the conserved orthologous set markers, genomic in situ hybridization (GISH), and meiotic chromosome pairing were used in this study. The unweighted pair-group method with arithmetical averages (UPGMA) analysis indicated that 7el1, derived from T. ponticum, and 7E(i), derived from T. intermedium, were the most closely related. 7el2, derived from T. ponticum, was relatively distant from the 7el1-7E(i) complex. While 7E(e), derived from T. elongatum, was more distantly related to 7el1, 7el2, and 7E(i). This is the first report showing that 7el1 and 7E(i) may be similar, which could be explained by the similar chromosome signal distribution revealed by GISH as well as UPGMA analysis revealed by both molecular markers and the highest frequency of meiotic pairing. The newly developed genome-specific molecular markers may be useful for marker-assisted selection of Lr19, Bdv3, and Fhblop. © 2015 S. Karger AG, Basel.

Perfilado de autor multilingüe en redes sociales a partir de n-gramas de caracteres y de etiquetas gramaticales

Directory of Open Access Journals (Sweden)

Carlos-Emiliano González-Gallardo

2016-07-01

Full Text Available En este artículo presentamos un algoritmo que combina las características estilísticas representadas por los n-gramas de caracteres y los n-gramas de etiquetas gramaticales (POS para clasificar documentos multilengua de redes sociales. En ambos grupos de n-gramas se aplicó una normalización dinámica dependiente del contexto para extraer la mayor cantidad de información estilística posible codificada en los documentos (emoticonos, inundamiento de caracteres, uso de letras mayúsculas, referencias a usuarios, ligas a sitios externos, hashtags, etc.. El algoritmo fue aplicado sobre dos corpus diferentes: los tweets del corpus de entrenamiento de la tarea Author Profiling de PAN-CLEF 2015 y el corpus de "Comentarios de la Ciudad de México en el tiempo" (CCDMX. Los resultados presentan una exactitud muy alta, cercana al 90%.

Extensión semántica del término “ojo” en español y chino

Directory of Open Access Journals (Sweden)

He, Yan

2017-07-01

Full Text Available Este artículo se ocupa del estudio de la extensión semántica de la palabra ojo en las expresiones fraseológicas en chino y en español desde una perspectiva subléxica, incluida en el modelo teórico del Lexicón Generativo. El análisis de los datos ha permitido probar que el sentido global que aportan las expresiones idiomáticas, tanto en chino como en español, surge de la extensión semántica de la palabra clave ojo mediante la materialización de la información potencialmente codificada en distintos qualia de dicha palabra. Con el presente trabajo se puede afirmar que las expresiones idiomáticas, que parecen idiosincrásicas, en realidad no lo son, y que pueden tener una explicación teórica.

A Escrita Feminina e a Tradição Literária

Directory of Open Access Journals (Sweden)

Magdala França Vianna

2008-04-01

Full Text Available Duas escritoras do Canadá contemporâneo, Audrey Thomas e Mary di Michele, reescrevendo o logos narrativo patriarcal—marca ocidental de exercício do poder— inscrevem por transgressão o lugar do feminino na textualidade tradicional codificada pelos interditos culturais do discurso masculino. A rasura praticada na matriz narrativa se evidencia pela prática de uma política literária de desvelamento do texto feminino latente, inscrito mas não escrito na ordem cultural produtora de uma história de exclusões. A adoção do modo confessional de narrar, permitindo a intertextualidade do autobiográfico e do ficcional, interfere no conceito que limita os espaços entre ficção e realidade e anula o hiato entre arte e vida, entre o mundo escrito e o não-escrito.

Cruetzfieldt Jakob Disease e sindromi correlate Il centro di riferimento Piemontese e la sua sorveglianza epidemiologica

Directory of Open Access Journals (Sweden)

G. Catapano

2003-05-01

Full Text Available

Le encefalopatie spongiformi trasmissibili (EST sono malattie del sistema nervoso dell’uomo, ad esito infausto. L’agente eziologico, la proteina prionica (PrP, può trasformarsi e accumularsi nella cellula nervosa causandone la morte. La scoperta negli anni ‘90 di una variante (v. Cruetzfieldt Jakob Disease, correlata all’encefalopatia spongiforme bovina, che colpisce soggetti sotto i 30 anni, ha aumentato l’interesse per le EST.

I paesi della comunità europea hanno adottano misure di controllo della carne bovina e si sono dotati di osservatori epidemiologici. Pertanto anche in Italia si è avuta l’esigenza di doverne disporre. Si è deciso quindi di istituire anche in Piemonte un centro per la sorveglianza e la notifica delle EST tramite sorveglianza epidemiologica e diagnosi, per mezzo di indagini molecolari e neuropatologiche con la successiva costituzione di una banca dati condivisa con altri centri di riferimento regionali, per la raccolta di dati clinici e strumentali dai reparti di neurologia piemontesi con ricovero in sede dei casi di EST giunti all’osservazione.

In sede vengono effettuati anche il prelievo, la raccolta, la conservazione di tessuti e altri materiali biologici e si svolgono successive indagini neurobiologiche quali:

 1 la ricerca della proteina 14 3 3 su liquor;

 2 determinazione genotipo del codone 129;

 3 determinazione della sequenza del gene della PrP,

 4 ricerca della PrP proteasi resistente a livello tissutale;

 5 determinazione del sottotipo di PrP. Le indagini neuropatologiche vengono effettuate in collaborazione con dipartimento di Neuroscienze dell’Università di Torino. Si intende inoltre fare promozione attiva delle attività e condivisione delle informazioni raccolte con altri centri.

Population genetics of the Chilean frog Batrachyla Leptopus (Leptodactylidae

Directory of Open Access Journals (Sweden)

J.R. Formas

2000-03-01

Full Text Available Electrophoretic variation of proteins encoded by 14 loci was analyzed in eight (five continental and three insular populations of the Chilean leptodactylid frog Batrachyla leptopus. The overall proportion of polymorphic loci was estimated to be 18.7% and the average number of alleles per locus, 1.2, while observed and expected heterozygosities were 1.7 and 5.1%, respectively. The estimated coefficient of genetic identity was 0.940; the corresponding figure for genetic distance was 0.063. F-statistics analysis showed a total inbreeding coefficient (Fit of 0.855 and high levels of genetic subdivision (Fst = 0.596 as well as of inbreeding within populations (Fis = 0.640. However, there was only a moderate level of genetic differentiation (Fst = 0.181 between the insular group of populations and the continental group.A variação eletroforética de proteínas codificadas por 14 loci foi analisada em oito populações (5 continentais e 3 insulares da rã leptodactilídea chilena Batrachyla leptopus. A proporção geral de loci polimórficos foi estimada como sendo de 18,7% e o número médio de alelos por loco, 1,2, enquanto que as heterozigosidades observada e esperada foram 1,7 e 5,1%, respectivamente. O coeficiente esperado de identidade genética foi 0,940; o número correspondente para a distância genética foi 0,063. A análise estatística F mostrou um coeficiente de endogamia total (Fit de 0,855 e altos níveis de subdivisão genética (Fst = 0,596, assim como de endogamia dentro das populações (Fis = 0,640. Contudo, houve apenas um nível moderado de diferenciação genética (Fst = 0,181 entre o grupo insular de populações e o grupo continental.

PAPP-A proteolytic activity enhances IGF bioactivity in ascites from women with ovarian carcinoma

DEFF Research Database (Denmark)

Thomsen, Jacob; Hjortebjerg, Rikke; Espelund, Ulrick

2015-01-01

Pregnancy-associated plasma protein-A (PAPP-A) stimulates insulin-like growth factor (IGF) action through proteolysis of IGF-binding protein (IGFBP)-4. In experimental animals, PAPP-A accelerates ovarian tumor growth by this mechanism. To investigate the effect of PAPP-A in humans, we compared.......03). Ascites was more potent than serum in activating the IGF-I receptor (IGF-IR) in vitro (+31%, P IGF-I, and lower levels of IGF-II (P ... of IGF-IR in all but one tumor, whereas all tumors expressed PAPP-A, IGFBP-4, IGF-I and IGF-II. Addition of recombinant PAPP-A to ascites increased the cleavage of IGFBP-4 and enhanced IGF-IR activation (P IGFs and these proteins...

First trimester serum placental growth factor and hyperglycosylated human chorionic gonadotropin are associated with pre-eclampsia: a case control study.

Science.gov (United States)

Keikkala, Elina; Koskinen, Sini; Vuorela, Piia; Laivuori, Hannele; Romppanen, Jarkko; Heinonen, Seppo; Stenman, Ulf-Håkan

2016-11-25

To study whether maternal serum hyperglycosylated human chorionic gonadotropin (hCG-h) improves first trimester prediction of pre-eclampsia when combined with placental growth factor (PlGF), pregnancy-associated plasma protein-A (PAPP-A) and maternal risk factors. Gestational-age-adjusted concentrations of hCG, hCG-h, PlGF and PAPP-A were analysed in serum samples by time-resolved immunofluorometric assays at 8-13 weeks of gestation. The case-control study included 98 women who developed pre-eclampsia, 25 who developed gestational hypertension, 41 normotensive women with small-for-gestational-age (SGA) infants and 177 controls. Of 98 women with pre-eclampsia, 24 women developed preterm pre-eclampsia (diagnosis factors.

7 CFR 1209.7 - Fiscal year.

Science.gov (United States)

2010-01-01

... 7 Agriculture 10 2010-01-01 2010-01-01 false Fiscal year. 1209.7 Section 1209.7 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS... CONSUMER INFORMATION ORDER Mushroom Promotion, Research, and Consumer Information Order Definitions § 1209...

7 CFR 652.7 - Quality assurance.

Science.gov (United States)

2010-01-01

... 7 Agriculture 6 2010-01-01 2010-01-01 false Quality assurance. 652.7 Section 652.7 Agriculture... assurance. (a) NRCS will review, in consultation with the Farm Service Agency, as appropriate, the quality... information obtained through its quality assurance process, documentation submitted by the technical service...

7 CFR 989.7 - Golden Seedless raisins.

Science.gov (United States)

2010-01-01

... 7 Agriculture 8 2010-01-01 2010-01-01 false Golden Seedless raisins. 989.7 Section 989.7... GROWN IN CALIFORNIA Order Regulating Handling Definitions § 989.7 Golden Seedless raisins. Golden Seedless raisins means raisins, the production of which includes soda dipping, sulfuring, and artificial...

7 CFR 932.7 - Variety group 2.

Science.gov (United States)

2010-01-01

... 7 Agriculture 8 2010-01-01 2010-01-01 false Variety group 2. 932.7 Section 932.7 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements... Regulating Handling Definitions § 932.7 Variety group 2. Variety group 2 means the following varieties and...

7 CFR 982.7 - To handle.

Science.gov (United States)

2010-01-01

... 7 Agriculture 8 2010-01-01 2010-01-01 false To handle. 982.7 Section 982.7 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements and... hazelnuts, inshell or shelled, into the channels of trade either within the area of production or from such...

7 CFR 612.7 - Forecast user responsibility.

Science.gov (United States)

2010-01-01

... 7 Agriculture 6 2010-01-01 2010-01-01 false Forecast user responsibility. 612.7 Section 612.7 Agriculture Regulations of the Department of Agriculture (Continued) NATURAL RESOURCES CONSERVATION SERVICE, DEPARTMENT OF AGRICULTURE CONSERVATION OPERATIONS SNOW SURVEYS AND WATER SUPPLY FORECASTS § 612.7 Forecast user responsibility. The forecast use...

7 CFR 1714.7 - Interest rate cap.

Science.gov (United States)

2010-01-01

... 7 Agriculture 11 2010-01-01 2010-01-01 false Interest rate cap. 1714.7 Section 1714.7 Agriculture... PRE-LOAN POLICIES AND PROCEDURES FOR INSURED ELECTRIC LOANS General § 1714.7 Interest rate cap. Except... section, or both the rate disparity test for the interest rate cap and the consumer income test set forth...

7 CFR 240.7 - Payments to States.

Science.gov (United States)

2010-01-01

... designated State Officials of a Payment Voucher on Letter of Credit (Treasury Form GFO 7578) in accordance... 7 Agriculture 4 2010-01-01 2010-01-01 false Payments to States. 240.7 Section 240.7 Agriculture... CHILD NUTRITION PROGRAMS CASH IN LIEU OF DONATED FOODS § 240.7 Payments to States. (a) Funds to be paid...

Application of FISH 7q in MDS patients without monosomy 7 or 7q deletion by conventional G-banding cytogenetics: does -7/7q- detection by FISH have prognostic value?

Science.gov (United States)

Ademà, Vera; Hernández, Jesús María; Abáigar, María; Lumbreras, Eva; Such, Esperanza; Calull, Anna; Dominguez, Esther; Arenillas, Leonor; Mallo, Mar; Cervera, José; Marugán, Isabel; Tormo, Mar; García, Francisca; González, Teresa; Luño, Elisa; Sanzo, Carmen; Martín, María Luisa; Fernández, Manuela; Costa, Dolors; Blázquez, Beatriz; Barreña, Beatriz; Marco, Fernando; Batlle, Ana; Buño, Ismael; Martínez-Laperche, Carolina; Noriega, Víctor; Collado, Rosa; Ivars, David; Carbonell, Félix; Vallcorba, Isabel; Melero, Josefa; Delgado, Elena; Vargas, María Teresa; Grau, Javier; Salido, Marta; Espinet, Blanca; Melero, Carme; Florensa, Lourdes; Pedro, Carmen; Solé, Francesc

2013-04-01

Chromosomal abnormalities are detected in 40-60% of patients with de novo myelodysplastic syndromes (MDS). This study used the FISH technique in 773 patients with de novo MDS without evidence of monosomy 7 (-7) or 7q deletion (7q-) by conventional G-banding cytogenetics (CC) to analyze their prognostic impact by FISH alone. FISH detected -7/7q- in 5.2% of patients. Presence of -7/7q- was associated with shorter overall survival than absence of such aberrations. Our results suggest that FISH 7q could be beneficial in patients with intermediate WHO morphologic risk stratification and no evidence of -7/7q- by CC. Copyright © 2012 Elsevier Ltd. All rights reserved.

De la tesis doctoral de Tiselius a la proteómica: setenta y cinco años de electroforesis de proteínas

Directory of Open Access Journals (Sweden)

Fernández Santarén, Juan

2004-02-01

Full Text Available El término «proteoma» se utilizó por primera vez en 1995 para describir las proteínas codificadas por un genoma. De forma involuntaria el proteoma se ha ido transformando en «proteomica», un conjunto de técnicas al que se ha elevado al rango de disciplina científica. ¿Qué es la proteomica? En esencia es el estudio, a gran escala, de la composición y propiedades de las proteínas que integran un sistema biológico. Su ejecución práctica se basa fundamentalmente en dos pilares. En primer lugar la separación de las mezclas complejas de proteínas, proceso que habitualmente se lleva a cabo mediante electroforesis bidimensional y en segundo lugar la identificación de las proteínas separadas para lo que actualmente se recurre a la espectrometría de masas.…

y la cultura tseltal

Directory of Open Access Journals (Sweden)

Antonio Paoli

2006-01-01

Full Text Available Se presenta un panorama general sobre las lenguas mayas; se ejemplifican brevemente sus formas de variación y se hace una sugerencia metódica para aproximarse paulatinamente a la comprensión de la lengua y la cultura tseltal. En cinco pequeñas frases, sugeridas para iniciarse en esta bella lengua, ya se alcanza a percibir el contraste contundente entre visiones del mundo codificadas en el tseltal y en el español. Este artículo nos introduce a algunas estructuras gramaticales claves del tseltal, comunes a la gran mayoría de las lenguas mayas. En la segunda mitad del artículo se nos aproxima a nociones que se traducen por “educación”, “autonomía” e “identidad” del mundo tseltal; esto se realiza mediante un análisis sociolingüístico y una aproximación humanística a este pueblo indígena del sureste mexicano.

Amino Acid Substitutions Improve the Immunogenicity of H7N7HA Protein and Protect Mice against Lethal H7N7 Viral Challenge.

Directory of Open Access Journals (Sweden)

Subaschandrabose Rajesh Kumar

Full Text Available Avian influenza A H7N7/NL/219/03 virus creates a serious pandemic threat to human health because it can transmit directly from domestic poultry to humans and from human to human. Our previous vaccine study reported that mice when immunized intranasally (i.n with live Bac-HA were protected from lethal H7N7/NL/219/03 challenge, whereas incomplete protection was obtained when administered subcutaneously (s.c due to the fact that H7N7 is a poor inducer of neutralizing antibodies. Interestingly, our recent vaccine studies reported that mice when vaccinated subcutaneously with Bac-HA (H7N9 was protected against both H7N9 (A/Sh2/2013 and H7N7 virus challenge. HA1 region of both H7N7 and H7N9 viruses are differ at 15 amino acid positions. Among those, we selected three amino acid positions (T143, T198 and I211 in HA1 region of H7N7. These amino acids are located within or near the receptor binding site. Following the selection, we substituted the amino acid at these three positions with amino acids found on H7N9HA wild-type. In this study, we evaluate the impact of amino acid substitutions in the H7N7 HA-protein on the immunogenicity. We generated six mutant constructs from wild-type influenza H7N7HA cDNA by site directed mutagenesis, and individually expressed mutant HA protein on the surface of baculovirus (Bac-HAm and compared their protective efficacy of the vaccines with Bac-H7N7HA wild-type (Bac-HA by lethal H7N7 viral challenge in a mouse model. We found that mice immunized subcutaneously with Bac-HAm constructs T143A or T198A-I211V or I211V-T143A serum showed significantly higher hemagglutination inhibition and neutralization titer against H7N7 and H7N9 viruses when compared to Bac-HA vaccinated mice groups. We also observed low level of lung viral titer, negligible weight loss and complete protection against lethal H7N7 viral challenge. Our results indicated that amino acid substitution at position 143 or 211 improve immunogenicity of H7N7HA

45 CFR 7.7 - Notice to employee of determination.

Science.gov (United States)

2010-10-01

... 45 Public Welfare 1 2010-10-01 2010-10-01 false Notice to employee of determination. 7.7 Section 7.7 Public Welfare DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL ADMINISTRATION EMPLOYEE INVENTIONS § 7.7 Notice to employee of determination. The employee-inventor shall be notified in writing of the...

Preparation and characterization of three 7Be targets for the measurement of the 7Be(n, p)7Li and 7Be(n, α)7Li reaction cross sections

Science.gov (United States)

Maugeri, E. A.; Heinitz, S.; Dressler, R.; Barbagallo, M.; Ulrich, J.; Schumann, D.; Colonna, N.; Köster, U.; Ayranov, M.; Vontobel, P.; Mastromarco, M.; Schell, J.; Correia, J. Martins; Stora, T.; n TOF Collaboration

2018-05-01

This manuscript describes the production of three targets obtained by implantation of different activities of 7Be into thin aluminium disks. Two of the produced targets were used to measure the 7Be(n, p)7Li cross section in the energy range of interest for the Big-Bang Nucleosynthesis. A third target was used to measure the cross sections of 7Be(n, p)7Li and 7Be(n, α)7Li nuclear reactions with cold and thermal neutrons, respectively. This paper describes also the characterization of the first two targets, performed after the neutron irradiation, in terms of implanted 7Be activities and spatial distributions.

7 CFR 945.7 - Certified seed potatoes.

Science.gov (United States)

2010-01-01

... 7 Agriculture 8 2010-01-01 2010-01-01 false Certified seed potatoes. 945.7 Section 945.7... Agreements and Orders; Fruits, Vegetables, Nuts), DEPARTMENT OF AGRICULTURE IRISH POTATOES GROWN IN CERTAIN... Certified seed potatoes. Certified seed potatoes means and includes all potatoes officially certified and...

7 CFR 1900.7 - Effect on other regulations.

Science.gov (United States)

2010-01-01

... 7 Agriculture 12 2010-01-01 2010-01-01 false Effect on other regulations. 1900.7 Section 1900.7 Agriculture Regulations of the Department of Agriculture (Continued) RURAL HOUSING SERVICE, RURAL BUSINESS... REGULATIONS GENERAL Delegations of Authority § 1900.7 Effect on other regulations. This subpart does not...

7Li--7Be experiment

International Nuclear Information System (INIS)

Rowley, J.K.

1978-01-01

An experiment to detect solar neutrinos by use of lithium is considered. The reaction employed is 7 Li(ν,e - ) 7 Be. The parameters of a possible experimental arrangement are described; chemical separation, backgrounds, and counting are discussed at some length. Despite the problems such an experiment still seems feasible. 5 figures, 6 tables

7 CFR 340.7 - Marking and identity.

Science.gov (United States)

2010-01-01

... 7 Agriculture 5 2010-01-01 2010-01-01 false Marking and identity. 340.7 Section 340.7 Agriculture Regulations of the Department of Agriculture (Continued) ANIMAL AND PLANT HEALTH INSPECTION SERVICE... identity. (a) Any regulated article to be imported other than by mail, shall, at the time of importation...

7 CFR 1499.7 - Transportation of goods.

Science.gov (United States)

2010-01-01

... 7 Agriculture 10 2010-01-01 2010-01-01 false Transportation of goods. 1499.7 Section 1499.7... Transportation of goods. (a) Shipments of donated commodities are subject to the requirements of 46 U.S.C. 55305 and 55314, regarding carriage on U.S.-flag vessels. (b) Transportation of donated commodities and...

7 CFR 1599.7 - Transportation of goods.

Science.gov (United States)

2010-01-01

... 7 Agriculture 10 2010-01-01 2010-01-01 false Transportation of goods. 1599.7 Section 1599.7... Transportation of goods. (a) Shipments of donated commodities are subject to the requirements of 46 U.S.C. 55305 and 55314, regarding carriage on U.S.-flag vessels. (b) Transportation of donated commodities and...

Protein labelling with avidin-biotin systems; Radiomarcado de proteinas con sistemas avidina-biotina

Energy Technology Data Exchange (ETDEWEB)

Hernandez B, B E

1998-06-01

The stability of connection in avidin-biotin system is very important due to the quadruple connections with avidin established with the same number of biotin molecules, which can amplify damage on cancer cells and increase specific activity of radio immuno conjugate in white cell. If between the first and second step (Ac Mo-biotin + avidin) enough time is left so that the monoclonal antibody accumulates in a therapeutic concentration required for the tumor or cancerous cells, then upon application of the third step (biotin-DTPA-{sup 153} Sm) it is hoped that in the first 30 minutes after application, only radioactivity remains with tumor. However, so that the amount radioactivity is enough to destroy a tumor, it would be necessary to use {sup 153} Sm with an activity of approximately 370 GBq (10 Ci)/ (mg). Since {sup 99m} Tc has similar chemistry to that of the {sup 188} Re, it is possible to propose their conjugates with biotin-avidin-Ac Mo-{sup 188} Re as a powerful option for therapeutic applications, this is, recommending the use of biotinylated labelled monoclonal antibody and the further injection of avidin to decrease of desirable effects on several other organs and bone marrow and high specific and selective action on tumor. On the other hand, we postulate the hypothesis in the sense that {sup 188} Re complexes tend to be more stable than those of {sup 99m} Tc, probably due to their metabolism, in which radioactivity of {sup 188} Re, not captured by tumor, is cleared easily from blood stream which results in a decrease of total and liver total dose in patient. (Author).

11 CFR 7.7 - Prohibited conduct-General.

Science.gov (United States)

2010-01-01

... Government efficiency or economy; (d) Losing independence or impartiality; (e) Making a Government decision... Employees or Commissioners § 7.7 Prohibited conduct—General. A Commissioner or employee shall avoid any...

Handbook of Accelerator Physics and Engineering (sections 2.7.1 - 2.7.5 and 7.6.2)

Energy Technology Data Exchange (ETDEWEB)

Roser, T.

1999-04-19

The sections written by this author are: 2.7.1- Thomas - BMT equation; 2.2.2- Spinor Algebra; 2.7.3- Spin Rotators and Siberian Snakes; 2.7.4- Ring with Spin Rotator and Siberian Snakes; 2.7.5- Depolarizing Resonances and Spin Flippers; & 7.6.2- Proton Beam Polarimeters

E7(7) constraints on counterterms in N=8 supergravity

International Nuclear Information System (INIS)

Beisert, N.; Elvang, H.; Freedman, D.; Kiermaier, M.; Morales, A.; Stieberger, S.

2010-01-01

We prove by explicit computation that 6-point matrix elements of D 4 R 4 and D 6 R 4 in N=8 supergravity have non-vanishing single-soft scalar limits, and therefore these operators violate the continuous E 7(7) symmetry. The soft limits precisely match automorphism constraints. Together with previous results for R 4 , this provides a direct proof that no E 7(7) -invariant candidate counterterm exists below 7-loop order. At 7-loops, we characterize the infinite tower of independent supersymmetric operators D 4 R 6 , R 8 , φ 2 R 8 , … with n>4 fields and prove that they all violate E 7(7) symmetry. This means that the 4-graviton amplitude determines whether or not the theory is finite at 7-loop order. We show that the corresponding candidate counterterm D 8 R 4 has a non-linear supersymmetrization such that its single- and double-soft scalar limits are compatible with E 7(7) up to and including 6-points. At loop orders 7,8,9 we provide an exhaustive account of all independent candidate counterterms with up to 16,14,12 fields, respectively, together with their potential single-soft scalar limits.

7 CFR 319.75-7 - Costs and charges.

Science.gov (United States)

2010-01-01

..., and shall be performed under the supervision of an inspector. Plant Protection and Quarantine will not... 7 Agriculture 5 2010-01-01 2010-01-01 false Costs and charges. 319.75-7 Section 319.75-7 Agriculture Regulations of the Department of Agriculture (Continued) ANIMAL AND PLANT HEALTH INSPECTION...

7 CFR 7.10 - Conduct of county convention.

Science.gov (United States)

2010-01-01

... 7 Agriculture 1 2010-01-01 2010-01-01 false Conduct of county convention. 7.10 Section 7.10 Agriculture Office of the Secretary of Agriculture SELECTION AND FUNCTIONS OF AGRICULTURAL STABILIZATION AND... other purpose. (e) The county committee shall give advance public notice of the county convention which...

7 CFR 7.6 - Determination of elective areas.

Science.gov (United States)

2010-01-01

... 7 Agriculture 1 2010-01-01 2010-01-01 false Determination of elective areas. 7.6 Section 7.6 Agriculture Office of the Secretary of Agriculture SELECTION AND FUNCTIONS OF AGRICULTURAL STABILIZATION AND... county committee shall give public notice of the community boundaries in advance of the election. ...

Mol 7C/6; Mol 7C/6

Energy Technology Data Exchange (ETDEWEB)

Aberle, J.; Schleisiek, K.; Schmuck, I.; Schmidt, L.; Romer, O.; Weih, G.

1995-08-01

The Mol 7C/6 coolant blockage experiment in the Belgian BR2 reactor yielded results different from Mol 7C experiments with low burnup pins: At 10% burnup local failure is not self-limiting, but requires active systems for detection and scram. The Mol 7C series was finished in 1991. In each of the test bundles Mol 7C/4, /5 and /6, 30 Mk I pins pre-irradiated in KNK II were used. The central blockage consisted of enriched UO{sub 2} covering 30 percent of the bundle cross-section, with a height of 40 mm. The most important system for timely detection of coolant blockages of the type studied in Mol 7C/6 is based on DND. (orig.)

7 CFR 760.7 - Other requirements for affected farmers.

Science.gov (United States)

2010-01-01

... 7 Agriculture 7 2010-01-01 2010-01-01 false Other requirements for affected farmers. 760.7 Section... Payments to Dairy Farmers for Milk § 760.7 Other requirements for affected farmers. An indemnity payment for milk may be made under this subpart to an affected farmer only under the following conditions: (a...

7 CFR 772.7 - Leasing minor program loan security.

Science.gov (United States)

2010-01-01

... 7 Agriculture 7 2010-01-01 2010-01-01 false Leasing minor program loan security. 772.7 Section 772..., DEPARTMENT OF AGRICULTURE SPECIAL PROGRAMS SERVICING MINOR PROGRAM LOANS § 772.7 Leasing minor program loan security. (a) Eligibility. The Agency may consent to the borrower leasing all or a portion of security...

7 CFR 319.55-7 - Importations by mail.

Science.gov (United States)

2010-01-01

... foreign mailing point by a special mailing tag directing the package to a Plant Protection and Quarantine... 7 Agriculture 5 2010-01-01 2010-01-01 false Importations by mail. 319.55-7 Section 319.55-7 Agriculture Regulations of the Department of Agriculture (Continued) ANIMAL AND PLANT HEALTH INSPECTION...

Structural aspects of crotalic venom proteins modified by ionizing radiation; Aspectos estruturais de proteinas do veneno crotalico modificadas por radiacao ionizante

Energy Technology Data Exchange (ETDEWEB)

Oliveira, Karina Corleto de

2010-07-01

Snake bites are a serious public health problem, especially in subtropical countries. In Brazil, the Ministry of Health notified around 26 000 accidents in 2008. The genus Crotalus (rattlesnakes) accounts for approximately 7% of the total, with a high mortality rate of 72% when untreated with the specific serum, the only effective treatment in case of snake bites. In Brazil, the serum is produced in horses which, despite the large size, have a reduced lifespan due to the high toxicity of the antigen. Ionizing radiation has proven to be an excellent tool for reducing the toxicity of venoms and isolated toxins, resulting in better immunogens for serum production, and contributing to the welfare of serum producing animals. Since the action of gamma radiation on venoms and toxins has not been yet fully clarified from the structural point of view, we proposed in this paper, to characterize two toxins of the species Crotalus durissus terrificus: crotoxin and crotamine. After isolation of the toxins of interest by chromatographic techniques, they were subjected to structural analysis with the application of the following methods: Fluorescence, Circular Dichroism, Differential Calorimetry and Infrared Spectroscopy. These tests showed that both crotamine as crotoxin when subjected to gamma radiation, showed changes in their structural conformation compared with the samples in the native state. Such changes probably occur in the secondary and tertiary structure and may explain the changes on the biological activity of these toxins. (author)

The 7-azanorbornane nucleus of epibatidine: 7-azabicyclo[2.2.1]heptan-7-ium chloride

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Sergey N. Britvin

2017-09-01

Full Text Available 7-Azabicyclo[2.2.1]heptane (7-azanorbornane is a bridged heterocyclic nucleus found in epibatidine, the alkaloid isolated from the skin of the tropical poison frog Epipedobates tricolor. Since epibatidine is known as one of the most potent acetylcholine nicotinic receptor agonists, a plethora of literature has been devoted to this alkaloid. However, there are no structural data on the unsubstituted 7-azanorbornane, the parent bicyclic ring of epibatidine and its derivatives. We herein present the structural characterization of the 7-azabicyclo[2.2.1]heptane parent ring as its hydrochloride salt, namely 7-azabicyclo[2.2.1]heptan-7-ium chloride, C6H12N+·Cl−. The compete cation is generated by a crystallographic mirror plane with the N atom lying on the mirror, as does the chloride anion. In the crystal, the cations are linked to the anions by N—H...Cl hydrogen bonds, which generate [001] chains.

Genomic association for sexual precocity in beef heifers using pre-selection of genes and haplotype reconstruction.

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Luciana Takada

Full Text Available Reproductive traits are of the utmost importance for any livestock farming, but are difficult to measure and to interpret since they are influenced by various factors. The objective of this study was to detect associations between known polymorphisms in candidate genes related to sexual precocity in Nellore heifers, which could be used in breeding programs. Records of 1,689 precocious and non-precocious heifers from farms participating in the Conexão Delta G breeding program were analyzed. A subset of single nucleotide polymorphisms (SNP located in the region of the candidate genes at a distance of up to 5 kb from the boundaries of each gene, were selected from the panel of 777,000 SNPs of the High-Density Bovine SNP BeadChip. Linear mixed models were used for statistical analysis of early heifer pregnancy, relating the trait with isolated SNPs or with haplotype groups. The model included the contemporary group (year and month of birth as fixed effect and parent of the animal (sire effect as random effect. The fastPHASE® and GenomeStudio® were used for reconstruction of the haplotypes and for analysis of linkage disequilibrium based on r2 statistics. A total of 125 candidate genes and 2,024 SNPs forming haplotypes were analyzed. Statistical analysis after Bonferroni correction showed that nine haplotypes exerted a significant effect (p<0.05 on sexual precocity. Four of these haplotypes were located in the Pregnancy-associated plasma protein-A2 gene (PAPP-A2, two in the Estrogen-related receptor gamma gene (ESRRG, and one each in the Pregnancy-associated plasma protein-A gene (PAPP-A, Kell blood group complex subunit-related family (XKR4 and mannose-binding lectin genes (MBL-1 genes. Although the present results indicate that the PAPP-A2, PAPP-A, XKR4, MBL-1 and ESRRG genes influence sexual precocity in Nellore heifers, further studies are needed to evaluate their possible use in breeding programs.

Mass-Production and Characterization of Anti-CD20 Monoclonal Antibody in Peritoneum of Balb/c Mice

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Leili Aghebati

2013-02-01

Full Text Available Purpose: Monoclonal antibodies are important tools are used in basic research as well as, in diagnosis, imaging and treatment of immunodeficiency diseases, infections and cancers. The purpose of this study was to produce large scale of monoclonal antibody against CD20 in order to diagnostic application in leukemia and lymphomas disorders. Methods: Hybridoma cells that produce monoclonal antibody against human CD20 were administered into the peritoneum of the Balb/c mice which have previously been primed with 0.5 ml Pristane. After twelve days, approximately 7 ml ascetic fluid was harvested from the peritoneum of each mouse. Evaluation of mAb titration was assessed by ELISA method. In the present study, we describe a protocol for large scale production of MAbs. Results: We prepared monoclonal antibodies (mAbs with high specificity and sensitivity against human CD20 by hybridoma method and characterized them by ELISA. The subclass of antibody was IgG2a and its light chain was kappa. Ascetic fluid was purified by Protein-A Sepharose affinity chromatography and the purified monoclonal antibody was conjugated with FITC and Immunofluorescence was done for confirming the specific binding. Conclusion: The conjugated monoclonal antibody could have application in diagnosis B-cell lymphomas, hairy cell leukemia, B-cell chronic lymphocytic leukemia, and melanoma cancer stem cells.

Mass-Production and Characterization of Anti-CD20 Monoclonal Antibody in Peritoneum of Balb/c Mice

Science.gov (United States)

Sineh sepehr, Koushan; Baradaran, Behzad; Majidi, Jafar; Abdolalizadeh, Jalal; Aghebati, leili; Zare Shahneh, Fatemeh

2013-01-01

Purpose: Monoclonal antibodies are important tools are used in basic research as well as, in diagnosis, imaging and treatment of immunodeficiency diseases, infections and cancers. The purpose of this study was to produce large scale of monoclonal antibody against CD20 in order to diagnostic application in leukemia and lymphomas disorders. Methods: Hybridoma cells that produce monoclonal antibody against human CD20 were administered into the peritoneum of the Balb/c mice which have previously been primed with 0.5 ml Pristane. After twelve days, approximately 7 ml ascetic fluid was harvested from the peritoneum of each mouse. Evaluation of mAb titration was assessed by ELISA method. In the present study, we describe a protocol for large scale production of MAbs. Results: We prepared monoclonal antibodies (mAbs) with high specificity and sensitivity against human CD20 by hybridoma method and characterized them by ELISA. The subclass of antibody was IgG2a and its light chain was kappa. Ascetic fluid was purified by Protein-A Sepharose affinity chromatography and the purified monoclonal antibody was conjugated with FITC and Immunofluorescence was done for confirming the specific binding. Conclusion: The conjugated monoclonal antibody could have application in diagnosis B-cell lymphomas, hairy cell leukemia, B-cell chronic lymphocytic leukemia, and melanoma cancer stem cells. PMID:24312821

Autoimmunity due to molecular mimicry as a cause of neurological disease.

Science.gov (United States)

Levin, Michael C; Lee, Sang Min; Kalume, Franck; Morcos, Yvette; Dohan, F Curtis; Hasty, Karen A; Callaway, Joseph C; Zunt, Joseph; Desiderio, Dominic; Stuart, John M

2002-05-01

One hypothesis that couples infection with autoimmune disease is molecular mimicry. Molecular mimicry is characterized by an immune response to an environmental agent that cross-reacts with a host antigen, resulting in disease. This hypothesis has been implicated in the pathogenesis of diabetes, lupus and multiple sclerosis (MS). There is limited direct evidence linking causative agents with pathogenic immune reactions in these diseases. Our study establishes a clear link between viral infection, autoimmunity and neurological disease in humans. As a model for molecular mimicry, we studied patients with human T-lymphotropic virus type 1 (HTLV-1)-associated myelopathy/tropical spastic paraparesis (HAM/TSP), a disease that can be indistinguishable from MS (refs. 5,6,7). HAM/TSP patients develop antibodies to neurons. We hypothesized these antibodies would identify a central nervous system (CNS) autoantigen. Immunoglobulin G isolated from HAM/TSP patients identified heterogeneous nuclear ribonuclear protein-A1 (hnRNP-A1) as the autoantigen. Antibodies to hnRNP-A1 cross-reacted with HTLV-1-tax, the immune response to which is associated with HAM/TSP (refs. 5,9). Immunoglobulin G specifically stained human Betz cells, whose axons are preferentially damaged. Infusion of autoantibodies in brain sections inhibited neuronal firing, indicative of their pathogenic nature. These data demonstrate the importance of molecular mimicry between an infecting agent and hnRNP-A1 in autoimmune disease of the CNS.

ATPasa de calcio en el sistema nervioso

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Myriam L. Velandia

2001-03-01

Full Text Available La bomba de calcio es una proteína integral de la membrana que regula el calcio libre citoplasmático en concentraciones menores de 0,1mM. En la mayoría de las células eucarióticas está ubicada en la membrana, en el plasmalema o en organelos como el retículo sarcoendoplásmico y los calciosomas. Su actividad está dada por la hidrólisis del ATP, la concentración del ion en el citoplasma y por otros factores que la regulan como la calmodulina, los fosfolípidos y las proteinas-cinasas. Por diferentes métodos, se ha detectado la ATPasa de calcio y su actividad tanto en tejido nervioso central y periférico, como en otros tejidos.

Composition and oil characteristics of apricot, peach and cherry kernel

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Lazos, Evangelos S.

1991-04-01

Full Text Available Se han obtenido huesos de albaricoque, melocotón y cereza de vegetales procesados y se han separado y analizado las pepitas después de eliminar las cascaras. El aceite crudo, extraído con éter de petróleo, fue 38’4, 48’0 y 26’0% de materia seca de pepitas de albaricoque, melocotón y cereza respectivamente; el contenido en proteina cruda fue 21’2, 26’7 y 25'3%; fibra bruta 5'0, 5’4 y 9’5%; hidratos de carbono totales 32'4, 16'0 y 34’5% de los cuales 9’3, 7’1 y 11’3% fueron azúcares reductores: y ceniza 3’0, 4’0 y 4’6% respectivamente. Se han encontrado en las pepitas cantidades considerables de K, P, Mg y Ca; se han tabulado valores para 15 minerales. Las características del aceite fueron: índice de iodo 105, 97, 116; índice de saponificación 195, 201, 198; materia insaponificable 0’95, 1’60, 3’12%; índice de refracción (40°C 1’4635, 1’4622, 1’4693; y gravedad específica (20°C 0’9227, 0’9158, 0’9419 para aceite de pepita de albaricoque, melocotón y cereza respectivamente. El ácido graso dominante fue el oleico (68’0, 69’0, 46’0% respectivamente seguido por el linoleico (24’2, 22’0, 41’6% respectivamente. Se han encontrado también en concentraciones traza C12:0, C14:0, C14:1, C15:0, C15:1, C16:0, C16:1, C17:0, C17:1, C18:0, C18:3, C20:0 y C20:1. El esterol principal fue el β-sitosterol alcanzando alrededor del 90%, seguido por el campesterol. Se han encontrado asimismo colesterol, estigmasterol, Δ⁵-avenasterol. Δ⁷-estigmasterol y Δ⁷-avenasterol.

Se han obtenido huesos de albaricoque, melocotón y cereza de vegetales procesados y se han separado y analizado las pepitas después de eliminar las cascaras. El aceite crudo, extraído con éter de petróleo, fue 38’4, 48’0 y 26’0% de materia seca de pepitas de albaricoque, melocotón y cereza respectivamente; el contenido en proteina cruda fue 21’2, 26’7 y 25'3%; fibra bruta

7 CFR 7.8 - Conduct of community committee elections.

Science.gov (United States)

2010-01-01

... 7 Agriculture 1 2010-01-01 2010-01-01 false Conduct of community committee elections. 7.8 Section 7.8 Agriculture Office of the Secretary of Agriculture SELECTION AND FUNCTIONS OF AGRICULTURAL... any other purpose. (c) The county committee shall give advance public notice of how, when, and where...

The (7,7) optics at CERN PS

CERN Document Server

Serluca, M; Efthymiopoulos, I; Sperati, F; Sterbini, G; Tecker, F; Zisopoulos, P

2017-01-01

The PS lattice is composed by one hundred combinedfunction magnets, which set the bare tune of the machineto (Qh,Qv) = (6.25, 6.28). Low energy quadrupoles areused at injection to move the tune in a limited working pointarea. In particular the vertical tune is moved below 6.25 toavoid the structural resonance 8Qv= 50 coupled with spacecharge, which leads to strong losses. In view of the highdemands in terms of beam brightness for LIU and HL-LHCprojects, the interest of exploring different integer tune work-ing area started during last years. During 2016, for the firsttime, it has been possible to explore the (7,7) tune workingarea at injection using the auxiliary circuits of the combinedfunction magnets. A finite-element magnetic model, underdevelopment, has been used to predict the required currentsin order to get the desired optical parameters. In this pa-per we present the results and issues encountered duringthe Machine Development (MD) studies about the injectionin the (7,7) area along with optics and be...

Reconfiguración del género en las performances clásicas. Androginia actoral.

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Miren Urquijo Arregui

2015-12-01

Full Text Available El género, producto del entorno social y factor decisivo en la comunicación, habla de nuestras identidades y proyecta nuestras imágenes codificadas como masculinas o femeninas. Las técnicas cotidianas de comportamiento corporal y los códigos vestimentarios regulan los roles de género, pero sirven asimismo para jugar con ellos y subvertirlos. Este artículo reflexiona sobre cómo la androginia, arquetipo de la reunión de lo masculino y lo femenino, es escenificada en el teatro kabuki, el teatro renacentista ingles y el teatro del siglo de Oro español, de manera que podemos hablar de esos actores y actrices que representan al otro sexo como un “tercer sexo”. Y así, transgreden simbólicamente la creencia de que el comportamiento está biológicamente y sexualmente programado. Al fin, en el cuerpo, -ficción social-, se abren múltiples posibilidades de reconfiguración del imaginario cultural genérico.

ESTRATEGIAS PARA HACER EL MEJOR USO DE LA PROTEINA EN RACIONES PARA VACAS LECHERAS

Science.gov (United States)

Dairy cows utilize feed CP with greater efficiency than other ruminants, but still excrete about 2 to 3 times more N in manure than they secrete in milk. This increases milk production costs plus environmental N pollution. Optimizing microbial protein formation in the rumen is the most effective way...

Proteinas do liquido cefalorraqueano: I. Estudo comparativo entre metodos de concentração

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Lucia M. Singer Vermes

1983-03-01

Full Text Available No intuito de escolher entre métodos de concentração, aplicáveis ao LCR, aquele que, não provocando desnaturação de proteínas, seja o mais exato, preciso e de fácil aplicação em laboratórios de rotina, foram estudados quatro procedimentos: diálise contra solução de goma-arábica, centrífugo-ultrafiltração, absorção de substâncias de baixo peso molecular por gel seco de poliacrilamida e ultrafiltração sob pressão positiva. Concluiu-se que a diálise contra solução de goma-arábica, por ser preciso, relativamente exato, simples e pouco oneroso, é o mais satisfatório dentre os métodos estudados.

Production of full length and splicing form of chymosin using pETexpression system in E-coli and investigation its enzyme activity and preplasmic secretion

Directory of Open Access Journals (Sweden)

M. Ahmadi Zeydabadi

2008-05-01

Full Text Available Introduction: Chymosin (Rennin EC 3.4.23.4 is an aspartyl proteinas (the major proteolyticenzyme in the fourth stomach of the unweaned calf that is formed by proteolytic activation fromzymogene prochymosin. The aim of his study was to produce the full length and splicing form ofchymosin using pETexpression system in E-coli and to assay the activity of expressed enzyme andpreplasmic secretion.Materials and Methods: The sense primer F-prochy(+ (5´-ggggccatgGCTGAGATCACCAGGAincluding NCOI restriction site. The anti sense R-prochy(- (5´-gggcggccgcGATGGCTTTGGCCAGC -3´ hybridizing to the C-terminal end of calf preprocymosincDNA and contains an additional NotI restriction site at its 5´-end . The cells were disrupted bysonication and proteins were purified by using Ni-NTA beads from Qiagen under native conditional.The preprochymosin and AS6 preprochymosin were activated at pH 4.7. The enzyme solutions werediluted 20-fold with 50 mM phosphate buffer .Results: Sequencing data analysis showed that the exon six has been spliced out and, therefore thegene product is 114 bp shorter in length, both chymosin forms were expressed together in E.coli.Under the same expression conditions, at least AS6 preprochymosin was produced 7-fold highexpression in comparison to a full-length recombinant chymosin. Following acid activation andneutralization, the purified fractions were tested in a qualitative milk clotting assay. The clottingactivity of preprochymosin and exon6-less preprochymosin were comparable.Conclusion: High expression of this alternatively expressed transcript in bacteria, and properfolding of the AS6 chymosin protein molecule in the absence of exon six are the two most importantaspects distinguished in this research.

7α-alkylation and 7,7-bis-alkylation of 20-hydroxyecdysone with propargyl bromide in a lithium-ammonia solution and catalytic reductive spirocyclization of 7,7-bis(2-propyn-1-yl)-14-deoxy-Δ(8(14))-20-hydroxyecdysone.

Science.gov (United States)

Galyautdinov, Ilgiz V; Khairullina, Zarema R; Sametov, Valery P; Muslimov, Zabir S; Khalilov, Leonard M; Odinokov, Victor N

2016-03-01

7α-Alkylation and 7,7-bis-alkylation of 20-hydroxyecdysone with propargyl bromide in a lithium-ammonia solution resulted in the formation of 7α-(2-propyn-1-yl)- and 7,7-bis(2-propyn-1-yl)-14-deoxy-Δ(8(14))-20-hydroxyecdysone in 92% and 75% yield respectively. Upon catalytic hydrogenation (10% Pd-C) of 7,7-bis(2-propyn-1-yl) derivative spirocyclization occurs by geminal 2-propyn-1-yl groups. Copyright © 2016 Elsevier Inc. All rights reserved.

Heterogeneous genetic diversity pattern in Plasmodium vivax genes encoding merozoite surface proteins (MSP) -7E, -7F and -7L.

Science.gov (United States)

Garzón-Ospina, Diego; Forero-Rodríguez, Johanna; Patarroyo, Manuel A

2014-12-13

The msp-7 gene has become differentially expanded in the Plasmodium genus; Plasmodium vivax has the highest copy number of this gene, several of which encode antigenic proteins in merozoites. DNA sequences from thirty-six Colombian clinical isolates from P. vivax (pv) msp-7E, -7F and -7L genes were analysed for characterizing and studying the genetic diversity of these pvmsp-7 members which are expressed during the intra-erythrocyte stage; natural selection signals producing the variation pattern so observed were evaluated. The pvmsp-7E gene was highly polymorphic compared to pvmsp-7F and pvmsp-7L which were seen to have limited genetic diversity; pvmsp-7E polymorphism was seen to have been maintained by different types of positive selection. Even though these copies seemed to be species-specific duplications, a search in the Plasmodium cynomolgi genome (P. vivax sister taxon) showed that both species shared the whole msp-7 repertoire. This led to exploring the long-term effect of natural selection by comparing the orthologous sequences which led to finding signatures for lineage-specific positive selection. The results confirmed that the P. vivax msp-7 family has a heterogeneous genetic diversity pattern; some members are highly conserved whilst others are highly diverse. The results suggested that the 3'-end of these genes encode MSP-7 proteins' functional region whilst the central region of pvmsp-7E has evolved rapidly. The lineage-specific positive selection signals found suggested that mutations occurring in msp-7s genes during host switch may have succeeded in adapting the ancestral P. vivax parasite population to humans.

OPNET simulation Signaling System No.7 (SS7) network interfaces

OpenAIRE

Ow, Kong Chung.

2000-01-01

This thesis presents an OPNET model and simulation of the Signaling System No.7 (SS7) network, which is dubbed the world's largest data communications network. The main focus of the study is to model one of its levels, the Message Transfer Part Level 3, in accordance with the ITU.T recommendation Q.704. An overview of SS7 that includes the evolution and basics of SS7 architecture is provided to familarize the reader with the topic. This includes the protocol stack, signaling points, signaling...

7 CFR 7.29 - Delegation of authority to Deputy Administrator.

Science.gov (United States)

2010-01-01

... 7 Agriculture 1 2010-01-01 2010-01-01 false Delegation of authority to Deputy Administrator. 7.29... STABILIZATION AND CONSERVATION STATE, COUNTY AND COMMUNITY COMMITTEES § 7.29 Delegation of authority to Deputy... any such office, county executive director, or other county employee, for any and all of the reasons...

Quality Assurance Tracking System - R7 (QATS-R7)

Data.gov (United States)

U.S. Environmental Protection Agency — This is metadata documentation for the Quality Assurance Tracking System - R7, an EPA Region 7 resource that tracks information on quality assurance reviews. Also...

HJURP regulates cellular senescence in human fibroblasts and endothelial cells via a p53-dependent pathway.

Science.gov (United States)

Heo, Jong-Ik; Cho, Jung Hee; Kim, Jae-Ryong

2013-08-01

Holliday junction recognition protein (HJURP), a centromere protein-A (CENP-A) histone chaperone, mediates centromere-specific assembly of CENP-A nucleosome, contributing to high-fidelity chromosome segregation during cell division. However, the role of HJURP in cellular senescence of human primary cells remains unclear. We found that the expression levels of HJURP decreased in human dermal fibroblasts and umbilical vein endothelial cells in replicative or premature senescence. Ectopic expression of HJURP in senescent cells partially overcame cell senescence. Conversely, downregulation of HJURP in young cells led to premature senescence. p53 knockdown, but not p16 knockdown, abolished senescence phenotypes caused by HJURP reduction. These data suggest that HJURP plays an important role in the regulation of cellular senescence through a p53-dependent pathway and might contribute to tissue or organismal aging and protection of cellular transformation.

Figure 7

Data.gov (United States)

U.S. Environmental Protection Agency — Two files provided. The ENS.tar file contains text data files (*.csv) used to create Figure 7 and Figure 8. The Figure7.txt is an R script that reads these files and...

Adsorption of 1,3-butadiene on Si(111)7x7 surface

International Nuclear Information System (INIS)

An, K.-S.; Kim, Y.; Baik, J.Y.; Park, C.-Y.; Kim, B.

2004-01-01

Full text: The adsorption of 1,3-butadiene (BD : C 4 H 10 ) on the Si(111)7x7 surface has been investigated using synchrotron radiation photoemission spectroscopy and scanning tunneling microscopy. BD adsorbs on the Si(111)7x7 surface at room temperature, not only adatom-rest-atom bridging but also adatom-adatom bridging type. In the valence band spectrum for the Si(111)7x7 surface, the adatom and rest-atom states were observed at the binding energies of about 0.2 and 0.8 eV, respectively. With increasing BD exposure, the adatom state is completely quenched at an initial exposure, while the rest-atom state disappears at higher exposure. This indicates two different reaction pathways of BD on the Si(111)7x7 surface. STM shows three different adsorption types on the 7x7 surface, two adatom-adatom bridging and one adatom-rest-atom bridging types. By comparing the valence band and Si 2p core level spectra with STM images, it was found that the chemical reactivity of BD molecule with the adatom-rest-atom pair is strongly higher than the adatom-adatom pair

New QC 7 tools

International Nuclear Information System (INIS)

1982-03-01

This book tells of new QC with 7 tools which includes TQC and new QC with 7 tools which is for better propel, what is QC method to think? what is new QC 7 tool ? like KJ law, PDPC law, arrow and diagram law, and matrix diagram law, application of new QC 7 tools such as field to apply, application of new QC 7 tools for policy management the method of new QC 7 tools including related regulations KJ law, matrix and data analysis, PDPC law and education and introduction of new QC 7 tools.

Regulation of CCR7-dependent cell migration through?CCR7 homodimer formation

OpenAIRE

Kobayashi, Daichi; Endo, Masataka; Ochi, Hirotaka; Hojo, Hironobu; Miyasaka, Masayuki; Hayasaka, Haruko

2017-01-01

The chemokine receptor CCR7 contributes to various physiological and pathological processes including T cell maturation, T cell migration from the blood into secondary lymphoid tissues, and tumor cell metastasis to lymph nodes. Although a previous study suggested that the efficacy of CCR7 ligand-dependent T cell migration correlates with CCR7 homo- and heterodimer formation, the exact extent of contribution of the CCR7 dimerization remains unclear. Here, by inducing or disrupting CCR7 dimers,...

Synthesis of unsaturated diethers 7,8- and 7,9-di(hydroxymethyl)decahydro-7,8- and 7,9-dicarba-nido-undecaborates(1-)

International Nuclear Information System (INIS)

Solomatina, A.I.; Komarova, L.G.; Rusanov, A.L.

1995-01-01

Dicarba-nido-undecaborate(1-) anions were obtained by treatment of diallyl ether of the 1,2-di(hydroxymethyl)-1,2-dicarba-closo-dodecaborane(12), 1,2-di(hydroxymethyl)-1,2-dicarba-closo-dodecaborane(12) and 1,7-di(hydroxymethyl)-1,7-dicarba-closo-dodecaborane(12) with ethanolic potassium hydroxide and subsequent interaction of the products with cesium and tetramethylammonium chlorides. 8 refs

7 CFR 1610.7 - Acquisition of certain exchange facilities.

Science.gov (United States)

2010-01-01

... Section 1610.7 Agriculture Regulations of the Department of Agriculture (Continued) RURAL TELEPHONE BANK, DEPARTMENT OF AGRICULTURE LOAN POLICIES § 1610.7 Acquisition of certain exchange facilities. In the interest of making optimum use of the Bank's loan funds, a Bank loan for the acquisition of exchange...

Serum protein inhibition of thyrotropin binding to human thyroid tissue

International Nuclear Information System (INIS)

Beall, G.N.; Chopra, I.J.; Solomon, D.H.; Kruger, S.R.

1978-01-01

We used a modificaton of the TSH radioreceptor assay to detect TSH-binding inhibition (TBI) activity in serum and serum fractions from normal subjects and patients with Graves' disease. TBI activity is present in normal IgG prepared by DEAE-Sephadex chromatography and in normal globulins prepared by precipitation at 1.6 M ammonium sulfate. Other normal serum proteins also had TBI activity when large concentrations were tested. Gel filtration chromatography and powder block electrophoresis were used to prepare fractions of normal and Graves' disease sera. In these fractions from normal serum, TBI activity was found in both γ-globulin and α-globulin-albumin fractions electrophoretically and in both 7S and 4S peaks from gel filtration. TBI activity from Graves' disease patients' sera was similarly distributed, but relatively more TBI accompanied the electrophoretic γ-globulins. Sepharose Protein-A and anti-IgG were used as immunoabsorbents to isolate and purify IgG from normal and Graves' disease sera. TBI activity in IgG was proportional to the IgG concentration, indicating that the TBI which migrates as a γ-globulin electrophoretically is an IgG and thus may possibly be an antibody. Inhibitory activity found in normal serum globulins and in the non-IgG fractions of both normal and abnormal sera seriously interferes with attempts to use the TSH radioreceptor assay to study the hypothesized anti-TSH receptor antibody in the serum of patients with Graves' disease

Implanted $^{7}$Be targets for the study of neutron interactions with $^{7}$Be

CERN Multimedia

The disagreement of the predicted abundance of primordial $^{7}$Li with the observed abundance is a longstanding problem in Big Bang Nucleosynthesis (BBN) theory (“Primordial $^{7}$Li problem”). While BBN theory correctly predicts the relative abundances of $^{2}$H/$^{1}$H, $^{3}$He/$^{1}$H and $^{4}$He/$^{1}$H (that vary over four orders of magnitudes), but it over-predicts the relative abundance of primordial $^{7}$Li/$^{1}$H by a factor of approximately 3-4 larger than observed (approximately 4-5$\\sigma$ discrepancy). Primordial $^{7}$Li is destroyed during the first 15 minutes primarily via the $^{7}$Li(p,$\\alpha$) reaction. Hence most of the primordial $^7$Li is predicted as the result of the (later when atoms are formed) electron capture $\\beta$-decay of the primordial $^{7}$Be that is produced primarily in the $^{3}$He($\\alpha$,$\\gamma$) )$^{7}$Be reaction. We propose to investigate the direct destruction of $^{7}$Be during (the first 15 minutes of) BBN via the $^{7}$Be(n,$\\alpha$) reaction to ch...

RELAP-7 Theory Manual

Energy Technology Data Exchange (ETDEWEB)

Berry, Ray Alden [Idaho National Lab. (INL), Idaho Falls, ID (United States); Zou, Ling [Idaho National Lab. (INL), Idaho Falls, ID (United States); Zhao, Haihua [Idaho National Lab. (INL), Idaho Falls, ID (United States); Zhang, Hongbin [Idaho National Lab. (INL), Idaho Falls, ID (United States); Peterson, John William [Idaho National Lab. (INL), Idaho Falls, ID (United States); Martineau, Richard Charles [Idaho National Lab. (INL), Idaho Falls, ID (United States); Kadioglu, Samet Yucel [Idaho National Lab. (INL), Idaho Falls, ID (United States); Andrs, David [Idaho National Lab. (INL), Idaho Falls, ID (United States)

2016-03-01

This document summarizes the physical models and mathematical formulations used in the RELAP-7 code. In summary, the MOOSE based RELAP-7 code development is an ongoing effort. The MOOSE framework enables rapid development of the RELAP-7 code. The developmental efforts and results demonstrate that the RELAP-7 project is on a path to success. This theory manual documents the main features implemented into the RELAP-7 code. Because the code is an ongoing development effort, this RELAP-7 Theory Manual will evolve with periodic updates to keep it current with the state of the development, implementation, and model additions/revisions.

Structure and Cancer Immunotherapy of the B7 Family Member B7x

Science.gov (United States)

Jeon, Hyungjun; Vigdorovich, Vladimir; Garrett-Thomson, Sarah C.; Janakiram, Murali; Ramagopal, Udupi A.; Abadi, Yael M.; Lee, Jun Sik; Scandiuzzi, Lisa; Ohaegbulam, Kim C; Chinai, Jordan M; Zhao, Ruihua; Yao, Yu; Mao, Ying; Sparano, Joseph A.; Almo, Steven C.; Zang, Xingxing

2014-01-01

SUMMARY B7x (B7-H4 or B7S1) is a member of the B7 family that can inhibit T cell function. B7x protein is absent in most normal human tissues and immune cells, but is overexpressed in human cancers and often correlates with negative clinical outcome. The expression pattern and function of B7x suggest that it may be a potent immunosuppressive pathway in human cancers. Here we determined the crystal structure of human B7x IgV domain at 1.59Å resolution and mapped the epitopes recognized by monoclonal antibodies. We developed a new in vivo system to screen therapeutic monoclonal antibodies against B7x, and found that the clone 1H3 significantly inhibited growth of B7x-expressing tumor in vivo via multiple mechanisms. Furthermore, the surviving mice given 1H3 treatment were resistant to tumor re-challenge. Our data suggest that targeting B7x on tumors is a promising cancer immunotherapy and humanized 1H3 may be efficacious for immunotherapy of human cancers. PMID:25437562

Structure and Cancer Immunotherapy of the B7 Family Member B7x

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Hyungjun Jeon

2014-11-01

Full Text Available B7x (B7-H4 or B7S1 is a member of the B7 family that can inhibit T cell function. B7x protein is absent in most normal human tissues and immune cells, but it is overexpressed in human cancers and often correlates with negative clinical outcome. The expression pattern and function of B7x suggest that it may be a potent immunosuppressive pathway in human cancers. Here, we determined the crystal structure of the human B7x immunoglobulin variable (IgV domain at 1.59 Å resolution and mapped the epitopes recognized by monoclonal antibodies. We developed an in vivo system to screen therapeutic monoclonal antibodies against B7x and found that the clone 1H3 significantly inhibited growth of B7x-expressing tumors in vivo via multiple mechanisms. Furthermore, the surviving mice given 1H3 treatment were resistant to tumor rechallenge. Our data suggest that targeting B7x on tumors is a promising cancer immunotherapy and humanized 1H3 may be efficacious for immunotherapy of human cancers.

Avian influenza H7N9/13 and H7N7/13: a comparative virulence study in chickens, pigeons, and ferrets.

Science.gov (United States)

Kalthoff, Donata; Bogs, Jessica; Grund, Christian; Tauscher, Kerstin; Teifke, Jens P; Starick, Elke; Harder, Timm; Beer, Martin

2014-08-01

Human influenza cases caused by a novel avian H7N9 virus in China emphasize the zoonotic potential of that subtype. We compared the infectivity and pathogenicity of the novel H7N9 virus with those of a recent European avian H7N7 strain in chickens, pigeons, and ferrets. Neither virus induced signs of disease despite substantial replication in inoculated chickens and rapid transmission to contact chickens. Evidence of the replication of both viruses in pigeons, albeit at lower levels of RNA excretion, was also detected. No clear-cut differences between the two H7 isolates emerged regarding replication and antibody development in avian hosts. In ferrets, in contrast, greater replication of the avian H7N9 virus than of the H7N7 strain was observed with significant differences in viral presence, e.g., in nasal wash, lung, and cerebellum samples. Importantly, both viruses showed the potential to spread to the mammal brain. We conclude that efficient asymptomatic viral replication and shedding, as shown in chickens, facilitate the spread of H7 viruses that may harbor zoonotic potential. Biosafety measures are required for the handling of poultry infected with avian influenza viruses of the H7 subtype, independently of their pathogenicity for gallinaceous poultry. This study is important to the field since it provides data about the behavior of the novel H7N9 avian influenza virus in chickens, pigeons, and ferrets in comparison with that of a recent low-pathogenicity H7N7 strain isolated from poultry. We clearly show that chickens, but not pigeons, are highly permissive hosts of both H7 viruses, allowing high-titer replication and virus shedding without any relevant clinical signs. In the ferret model, the potential of both viruses to infect mammals could be demonstrated, including infection of the brain. However, the replication efficiency of the H7N9 virus in ferrets was higher than that of the H7N7 strain. In conclusion, valuable data for the risk analysis of low

7 CFR 1126.7 - Pool plant.

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2010-01-01

... excluded from the supply plant's shipments in computing the plant's shipping percentage. (d) A plant... part, or the plant has automatic pooling status under the other Federal order; and (7) That portion of...

LAS PROTEINAS SEMINALES DEL MANI (ARACHIS HYPOGAEA, LEGUMINOSAE y SU RELACION CON LAS CATEGORIAS INFRAESPECIFICAS

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N R Grosso

1994-01-01

Full Text Available Las proteínas seminales de 122 muestras diferentes de Arachis hypogaea L. originarios de Bolivia, Perú y Ecuador fueron estudiadas por electroforesis en gel de poliacrilamida.Se detectaron siete bandas constantes y 27 bandas inconstantes. Los resultados de las últimas se utilizaron para analizar las similitudes entre las muestras empleando el coeficiente de Jaccard y el método de ligamiento promedio de la media aritmética no ponderada(UPGMA.Las proteínas seminales permitieron separar totalmente la subespecies de A.hypogaea y las variedades en menor medida.

Produção de enzimas lignoceluloliticas e proteinas unicelulares por chrysonilia sitophila TFB 27441

OpenAIRE

Jaime Patricio Rodrigues Gutierrez

1987-01-01

Resumo: Estudou-se as enzimas celulolíticas e lignolíticas produzidas pelo microorganismo Chrysonlila sitophila TFB-27441 que foi isolado do trato intestinal de um inseto xilófago. A produção de celulases foi estudada utilizando como fonte de carbono no meio de cultivo gllcose, a-celuIose e a-celulose pré-tratada fotoquimicamente. As enzimas estudadas foram b-D-glicosidase, endo-1,4-b-D-glicanase e a atividade cetulolítica total foi estimada utilizando papel de filtro como substrato. A ativid...

Ab-initio study of pure sup 7 sup 7 Se and sup 1 sup 2 sup 5 Te systems and of the sup 7 sup 7 Se nuclear quadrupole interaction in tellurium

CERN Document Server

Oh, Y K; Cho, H S

1999-01-01

Using the Hartree-Fock cluster procedure, we have studied the electric-field gradient tensors at the nuclear sites of sup 7 sup 7 Se and sup 1 sup 2 sup 5 Te in pure sup 1 sup 2 sup 5 Te systems and in tellurium crystalline system's with a sup 7 sup 7 Se impurity. From the results for the pure systems, sup 7 sup 7 Se in selenium and sup 1 sup 2 sup 5 Te in tellurium, using the observed quadrupole moments: Q( sup 7 sup 7 Se) 0.75 +- 0.07 barns and Q( sup 1 sup 2 sup 5 Te) = 0.35 +- 0.04 barns. Comparison is made with earlier values obtained by different methods. Using our calculated values of Q and the results of a study of the field-gradient tensors for sup 7 sup 7 Se in tellurium, the theoretical values of the quadrupole coupling constants are found to agree, within about 7 percent, with experiment. The calculated asymmetry parameters are also found to be in reasonable agreement with the experiment values, although the agreement not as close as in the case of the quadrupole -coupling constants. Directions fo...

Electrochemical thiocyanation of dodecahydro-7,8-dicarba-nido-undecaborate and 7,8-dimethyldecahydro-7,8-dicarba-nido-undecaborate monoanions

International Nuclear Information System (INIS)

Rudakov, D.A.; Shirokij, V.L.; Potkin, V.I.; Majer, N.A.; Bragin, D.I.; Petrovskij, P.V.; Sivaev, I.B.; Bregadze, V.I.; Kisin, A.V.

2005-01-01

Electrochemical thiocyanation of the dodecahydro-7,8-dicarba-nido-undecaborate and 7,8-dimethyldecahydro-7,8-dicarba-nido-undecaborate monoanions afforded thiocyanate derivatives of these compounds, which were isolated as alkylammonium salts. The structures of the synthesized compounds were determined by the data from IR, 1 H and 11 B NMR, and 11 B- 11 B NMR COSY spectroscopy [ru

7 CFR 801.7 - Reference methods and tolerances for near-infrared spectroscopy (NIRS) analyzers.

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2010-01-01

... 7 Agriculture 7 2010-01-01 2010-01-01 false Reference methods and tolerances for near-infrared spectroscopy (NIRS) analyzers. 801.7 Section 801.7 Agriculture Regulations of the Department of Agriculture...), DEPARTMENT OF AGRICULTURE OFFICIAL PERFORMANCE REQUIREMENTS FOR GRAIN INSPECTION EQUIPMENT § 801.7 Reference...

Resistive ballooning modes in W7-AS and W7-X

International Nuclear Information System (INIS)

Kaiser, R.

1993-01-01

'Critical' pressure gradients due to resistive ballooning modes and their growth rates were computed for the W7-AS stellarator and for a HELIAS configuration with W7-X parameters, and the two configurations were compared. The results are based on the evaluation of a fourth order magnetic differential equation along closed magnetic field lines. The numerical procedure applying a variational approach uses the 'Garching resistive ballooning code', GARBO, which was originally developed for the stability analysis of axisymmetric plasmas. Concerning purely growing modes, this analysis shows that the favourable stability properties of W7-X, already optimized with respect to ideal ballooning modes, persist in the resistive regime: the destabilizing effect of resistivity is largely compensated by the stabilizing contribution of plasma compression. As a consequence, the ideal β limit continues only moderately shifted in the resistive case and likewise ideal ballooning stable equilibria (up to β 0 ≅ 5%) do not become resistively unstable. The situation is different for W7-AS. Greater resistive effects (in comparison with W7-X) are found in a configuration that is already ideally much more unstable. A basic feature in resistive calculations is the occurrence of overstable modes. These modes no longer show a stability threshold and, for realistic values of pressure and resistivity, linear instability is obtained for W7-AS as well as for W7-X, with growth rates and oscillation frequencies in the kilo-Hertz range. (author). 29 refs, 13 figs

High-efficiency Agrobacterium-mediated transformation of Norway spruce (Picea abies) and loblolly pine (Pinus taeda)

Science.gov (United States)

Wenck, A. R.; Quinn, M.; Whetten, R. W.; Pullman, G.; Sederoff, R.; Brown, C. S. (Principal Investigator)

1999-01-01

Agrobacterium-mediated gene transfer is the method of choice for many plant biotechnology laboratories; however, large-scale use of this organism in conifer transformation has been limited by difficult propagation of explant material, selection efficiencies and low transformation frequency. We have analyzed co-cultivation conditions and different disarmed strains of Agrobacterium to improve transformation. Additional copies of virulence genes were added to three common disarmed strains. These extra virulence genes included either a constitutively active virG or extra copies of virG and virB, both from pTiBo542. In experiments with Norway spruce, we increased transformation efficiencies 1000-fold from initial experiments where little or no transient expression was detected. Over 100 transformed lines expressing the marker gene beta-glucuronidase (GUS) were generated from rapidly dividing embryogenic suspension-cultured cells co-cultivated with Agrobacterium. GUS activity was used to monitor transient expression and to further test lines selected on kanamycin-containing medium. In loblolly pine, transient expression increased 10-fold utilizing modified Agrobacterium strains. Agrobacterium-mediated gene transfer is a useful technique for large-scale generation of transgenic Norway spruce and may prove useful for other conifer species.

Brucella Intracellular Life Relies on the Transmembrane Protein CD98 Heavy Chain.

Science.gov (United States)

Keriel, Anne; Botella, Eric; Estrach, Soline; Bragagnolo, Gabriel; Vergunst, Annette C; Feral, Chloe C; O'Callaghan, David

2015-06-01

Brucella are intracellular bacterial pathogens that use a type IV secretion system (T4SS) to escape host defenses and create a niche in which they can multiply. Although the importance of Brucella T4SS is clear, little is known about its interactions with host cell structures. In this study, we identified the eukaryotic protein CD98hc as a partner for Brucella T4SS subunit VirB2. This transmembrane glycoprotein is involved in amino acid transport, modulation of integrin signaling, and cell-to-cell fusion. Knockdown of CD98hc expression in HeLa cells demonstrated that it is essential for Brucella infection. Using knockout dermal fibroblasts, we confirmed its role for Brucella but found that it is not required for Salmonella infection. CD98hc transiently accumulates around the bacteria during the early phases of infection and is required for both optimal bacterial uptake and intracellular multiplication of Brucella. These results provide new insights into the complex interplay between Brucella and its host. © The Author 2014. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

Revista de tesis de la Facultad de Medicina de Bogotá

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Facultad de Medicina Revista

1943-07-01

Full Text Available Excitación de la contractilidad uterina por el bicarbonato de sodio. Tesis de grado declarada meritoria. 1942. - Por Álvaro Combariza Vargas. Conclusiones / Contribución al estudio y tratamiento de los epiteliomas del labio. Tesis de grado, declarada meritoria. 1942. - Por Aurelio Pabón Rodríguez / Modificaciones de las proteinas totales del suero sanguineo y de la gravedad especifica de la sangre en el acto  quirúrgico. Tesis de grado, declarada meritoria. 1942. - Por Leonidas Albornoz Medina / Venografia central y periferica. Tesis de grado. 1942. - Por Guillermo Sierra E. / Tratamiento del chancro blando y del linfogranuloma venereo. Tesis de grado. 1942. - Por Ernesto Martínez Capella / Computo diferencial leucocitario de Schilling, y eritrosedimentación en la enfermedad de Hansen. Tesis de grado, 1942. - Por Jorge Jiménez Gandica

Aislamiento y purificación de prolactina humana. ii. purificación y caracterización parcial

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Stella Carrasco de Rodríguez

2009-07-01

Full Text Available En este trabajo se describe un sistema cromatográfico en dos etapas que permite la purificación de prolactina humana (hPRL, a partir del residuo obtenido después de la extracción con solución salina de hipófisis conservadas congeladas. La PRL se solubilizó en acetato de amonio 50 mM, pH 10 y se purificó por medio de cromatografía de interacción hidrofóbica, sobre el soporte Fenil-Sepharosa en presencia de un gradiente lineal de acetonltrílo (0-40%. La fracción con actividad de PRL se aplicó a una columna de DEAE-Celulosa en presencia de acetonítrilo (20% y se eluyó con un gradiente salino. El rendimiento del proceso fue de 7.75 mg PRL por 100 glándulEis, lo que corresponde a una recuperación global de 33%. Por radioinmunoanálisis especifico (RÍA se encontró una potencia de 10.6 Ul/mg de proteina en la preparación final. El análisis electroforético mostró dos componentes principales de pesos 27.000 Dattons, que corresponde al monómero principal y 64.000 Daltons, que sugiere una forma agregada o glicosilada, cuya existencia ha sido reportada en el suero. La presencia de isohormonas también fue detectada por el análisis de electroenfoque, encontrándose un valor de punto isoeléctrico (PI de 5.7 para la especie principal. Este comportamiento también se observó con la preparación internacional de referencia usada en este trabajo.

Molecular characterization of Cryptosporidium spp. from HIV infected patients from an urban area of Brazil Caracterização molecular de Cryptosporidium spp. de pacientes de área urbana do Brasil infectados por HIV

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Patrícia de Lucca

2009-12-01

Full Text Available Cryptosporidium spp. are important cause of enteric disease in humans, but may also infect animals. This study describes the relative frequency of several Cryptosporidium species found in human specimens from HIV infected patients in the São Paulo municipality obtained from January to July 2007. Sequence analysis of the products of nested-PCR based on small subunit rRNA and Cryptosporidium oocyst wall protein coding genes revealed 17 (63.0% isolates of C. hominis, four (14.8% C. parvum, five (18.5% C. felis and one (3.7% C. canis. These findings suggest that, in urban environments of Brazil, the cat adapted C. felis may play a potential role in the zoonotic transmission of cryptosporidiosis whereas the anthroponotic transmission of cryptosporidiosis caused by C. hominis seems to predominate.Cryptosporidium spp. são importantes causas de doenças entéricas em humanos, mas podem também ser encontrados em animais. O presente estudo descreve a frequência relativa de diversas espécies de Cryptosporidium em amostras de humanos da cidade de São Paulo, Brasil, obtidas de janeiro a julho de 2007. Análises de sequências de produtos de nested PCR direcionadas ao genes codificadores da menor unidade ribosomal e da proteina de parede de oocistos revelaram 17 (63,0% isolados de C. hominis, quatro (14,8% C. parvum, cinco (18,5% C. felis, e um (3,7% C. canis. Estes resultados sugerem que, em ambientes urbanos no Brasil, o genótipo adaptado ao gato pode desempenhar potencial papel na transmissão zoonótica de criptosporidiose, enquanto a transmissão antroponótica da criptosporidiose causada pelo C. hominis parece predominar.

Low-field spin dynamics of Cr7Ni and Cr7Ni-Cu -Cr 7Ni molecular rings as detected by μ SR

Science.gov (United States)

Sanna, S.; Arosio, P.; Bordonali, L.; Adelnia, F.; Mariani, M.; Garlatti, E.; Baines, C.; Amato, A.; Sabareesh, K. P. V.; Timco, G.; Winpenny, R. E. P.; Blundell, S. J.; Lascialfari, A.

2017-11-01

Muon spin rotation measurements were used to investigate the spin dynamics of heterometallic Cr7Ni and Cr7Ni -Cu-Cr7Ni molecular clusters. In Cr7Ni the magnetic ions are arranged in a quasiplanar ring and interact via an antiferromagnetic exchange coupling constant J , while Cr7Ni -Cu-Cr7Ni is composed of two Cr7Ni linked by a bridging moiety containing one Cu ion, that induces an inter-ring ferromagnetic interaction J'≪J . The longitudinal muon relaxation rate λ collected at low magnetic fields μ0H BPP)-like heuristic fitting model that takes into account of a distribution of electronic spin characteristic times for T >5 K, while the shoulder presented by Cr7Ni can be reproduced by a BPP function that incorporates a single electronic characteristic time theoretically predicted to dominate for T <5 K. The flattening of λ (T ) in Cr7Ni -Cu-Cr7Ni occurring at very low temperature can be tentatively attributed to field-dependent quantum effects and/or to an inelastic term in the spectral density of the electronic spin fluctuations.

Study of sodium arsenite induced biochemical changes on certain biomolecules of the freshwater catfish Clarias batrachus

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Randhir Kumar

2012-01-01

Full Text Available Toxic impact of sublethal concentration (1 mg/L; 5% of 96h LC50 value of sodium arsenite (NaAsO2 on certain biomolecules (proteins, nucleic acids, lipids, and glycogen of five tissue components (muscles, liver, brain, skin, and gills of the freshwater catfish Clarias batrachus was analysed. The important toxic manifestations include marked decrease in the concentration of proteins (21.72-45.42% in muscles; 3.42-53.94% in liver; 15.39-45.42% in brain; 15.40-4.00% in skin and 11.35-64.13% in gills, DNA (0.55-22.95% in muscles; 8.33-14.06% in liver; 5.30-18.40% in brain; 13.57-52.80% in skin; and 12.38-31.01% in gills, RNA (42.68-76.16% in muscles; 10.68-39.75% in liver; 5.66-29.05% in brain; 7.72-27.93% in skin and 21.47-44.38% in gills and glycogen (24.00-51.72% in muscles; 49.11-72.45% in liver; 11.49-26.03% in brain; 26.13-38.05% in skin and 17.80-37.97% in gills. Excepting liver where the lipid content increases (15.82-24.13%, the fat content also showed depletion in their concentration (10.40-29.83% in muscles; 8.30-34.45% in brain; 8.94-31.47% in skin and 12.75-28.86% in gills, in the rest of the organ systems.Foi analisado o impacto tóxico da concentração subletal (1 mg/L; 5% do valor de LC50 de 96h do arsenito de sódio (NaAsO2 sobre certas biomoléculas (proteinas, ácidos nucleicos, lipídios e glicogênio de cinco tecidos (músculos, fígado, cérebro, pele e brânquias do bagre Clarias batrachus. As manifestações tóxicas importantes incluiram o decréscimo acentuado na concentração de proteinas (21,72-45,42% nos músculos; 3,42-53,94% no fígado; 15,39-45,42% no cérebro; 15,40-4,00% na pele e 11,35-64,13% nas brânquias, DNA (0,55-22,95% nos músculos; 8,33-14,06% no fígado; 5,30-18,40% no cérebro; 13,57-52,80% na pele e 12,38-31,01% nas brânquias, RNA (42,68-76,16% nos músculos; 10,68-39,75% no fígado; 5,66-29,05% no cérebro; 7,72-27,93% na pele e 21,47-44,38% nas brânquias e glicogênio (24,00-51,72% nos músculos; 49

7 CFR 771.7 - Equal opportunity and non-discrimination requirements.

Science.gov (United States)

2010-01-01

... to discrimination on the basis of race, religion, color, national origin, gender, or other prohibited... 7 Agriculture 7 2010-01-01 2010-01-01 false Equal opportunity and non-discrimination requirements... opportunity and non-discrimination requirements. No recipient of a boll weevil eradication loan shall directly...

Drycleaner Database - Region 7

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U.S. Environmental Protection Agency — THIS DATA ASSET NO LONGER ACTIVE: This is metadata documentation for the Region 7 Drycleaner Database (R7DryClnDB) which tracks all Region7 drycleaners who notify...

The importance of the tensor interaction in the (7Li, 7Be) reaction

International Nuclear Information System (INIS)

Dodd, A.C.; Clarke, N.M.; Coopersmith, J.; Griffiths, R.J.; Pearce, K.I.; Stanley, B.; Cook, J.

1985-01-01

Data for the 28 Si( 7 Li, 7 Be) 28 Al reaction at 72 MeV and for the 26 Mg( 7 Li, 7 Be) 26 Na reaction at 88 MeV are presented together with one-step DWBA calculations using microscopic form factors. The tensor interaction is shown to be important to explain the structureless nature of the angular distributions. (author)

Causas básicas e associadas de morte por Aids, Estado de São Paulo, Brasil, 1998

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Santo Augusto Hasiak

2000-01-01

Full Text Available OBJETIVOS: Descrever o padrão da mortalidade devida a Aids segundo causas básica e associadas de morte no Estado de São Paulo, em 1998. MÉTODOS: Os dados sobre a mortalidade e a população residente no Estado de São Paulo, SP, para 1998, foram obtidos na Fundação Sistema Estadual de Análise de Dados (Seade. As causas de morte foram codificadas pelas disposições da Décima Revisão da Classificação Estatística Internacional de Doenças e Problemas Relacionados à Saúde. Os registros de Aids como causa básica e associada de morte foram recuperados e revistos. RESULTADOS: A Aids foi a causa básica em 4.619 mortes, correspondendo à décima causa (2,0% e ao coeficiente de mortalidade de 13,1 por 100.000 habitantes. As razões das mortes e os respectivos coeficientes entre homens e mulheres foram de 2,4 e 2,5. A Aids foi a segunda causa entre os homens de 20 a 34 anos de idade e entre as mulheres de 25 a 34. A idade média ao morrer entre as mulheres (34,1±12,2 anos foi estatisticamente menor que a dos homens (36,4±10,7 anos -- p<0,001. As principais causas associadas em mortes por Aids foram a insuficiência respiratória (36,1%, pneumonias (27,0%, tuberculose (19,6%, septicemias (18,6%, toxoplasmose (12,2%, pneumonia por P. carinii (8,3% e caquexia (7,9%. A Aids apresentou-se como causa associada em outras 84 mortes. As principais causas básicas destas mortes foram neoplasias malignas (28/84, afecções devidas ao uso do álcool (23/84 e diabetes mellitus (7/84. A idade média ao morrer por Aids como causa básica (35,7±11,2 anos foi estatisticamente menor que a idade média nas mortes em que Aids foi mencionada como causa associada (39,9±11,8 anos -- p<0,001. CONCLUSÕES: As causas múltiplas de morte resgatam parcialmente a história natural da Aids e oferecem subsídios para medidas preventivas adequadas e específicas.

Relación de la transición A G en la posición –21 del intrón 10 del gen NCF-2 con la expresión de la proteína p67-phox

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Pablo Patiño

2001-04-01

Full Text Available

El sistema NADPH oxidasa de las células fagocíticas es esencial
para la producción de metabolitos reactivos de oxígeno que tienen
acción microbicida importante. La proteína p67-phox, codificada por
el gen NCF-2, tiene un papel importante en este sistema (1. Previamente nuestro grupo reportó algunos cambios nucleotídicos en regiones no codificadoras del gen NCF-2. Uno de estos corresponde a la
transición de una adenina (A por una guanina (G en el intrón 10, 21
nucleótidos antes del inicio del exón 11, sitio importante para la eliminación de este intrón y unión de los exones 10 y 11 durante el procesamiento del ARNm.
En este proyecto se pretende determinar si existe alguna modificación
en la expresión de p67-phox inducida por la transición A -►G en la posición -21 del intrón 10 en el gen NCF-2.

LA PROTEOMICA EN LA ERA POSTGENOMICA

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Catalina Arevalo-Ferro

2009-09-01

Full Text Available El principal desafío de la biología moderna es entender la expresión, función y regulación del conjunto completo de proteínas codificadas por un organismo, lo cual describe el objetivo del nuevo campo de la proteómica. Las proteínas son las efectoras del trabajo celular, por ello el estudio de sus perfiles globales de expresión y de sus cambios bajo determinadas condiciones fisiológicas o patológicas, permite entender la red compleja de interacciones en que se basa el funcionamiento de una célula. La electroforesis en dos dimensiones (2D-PAGE es una de las técnicas empleadas con mayor frecuencia en proteómica, debido en gran medida a su alta reproducibilidad y capacidad para resolver simultáneamente miles de proteínas en un solo procedimiento y para detectar modificaciones post y co-traduccionales imposibles de predecir a partir de la secuencia genómica. Sus aplicaciones incluyen el análisis de proteomas, señalización, detección de marcadores de enfermedades y cáncer.

Una historia del delito político. Sedición, traición y rebelión en la justicia penal neogranadina (1832-1842

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Gilberto Enrique Parada García

2012-07-01

Full Text Available El artículo presenta una investigación contextualizada historiográficamente en la historia social del derecho, cuyo tema de análisis es un tipo de enfrentamiento que se originó a partir del funcionamiento de la justicia penal neogranadina. Concretamente, analiza las resistencias que surgieron en la sociedad neogranadina durante la década de 1830, a partir de la puesta en marcha de leyes penales codificadas para contener delitos políticos. La perspectiva teórica apunta a que el Estado, específicamente representado por los gobiernos de esa década, asume la ley penal como herramienta del control político; esta maniobra genera tensiones que se manifiestan en opiniones jurídicas divergentes. A través de las fuentes estudiadas: leyes, decretos, expedientes, prensa, líbelos, hojas sueltas y panfletos, se evidencia un nuevo campo de batalla de la justicia penal, en el cual se señala un escenario extrajurídico en el que se disuelven litigios, en el marco de las crisis políticas generadas por la sedición y la guerra.

Factores que determinaron una educación física y deportiva de género durante el franquismo

Directory of Open Access Journals (Sweden)

uan Carlos Manrique Arribas

2018-03-01

Full Text Available El presente artículo pretende, desde una reflexión de la situación que vivió y experimentó la mujer en la sociedad franquista y mediante una revisión documental sobre el tema, abordar el estilo de Educación Física y deportiva que se propuso durante el franquismo. Asimismo, queremos dar a conocer cuáles fueron los fundamentos ideológicos que determinaron una clara educación de género. En concreto, nos detendremos en los valores que se asignaron a la mujer para establecer las relaciones oportunas con el modelo de actividad física que se la propuso. Las normas sociales fueron impuestas desde las altas esferas, sin posibilidad de réplica, por lo que sus miembros tuvieron que asumirlas y acatarlas. Las relaciones entre ambos sexos quedaron así codificadas y registradas en la mente de las personas, refrendadas por un proceso propagandístico y formativo; sobre todo a través de la educación, y en concreto con la Educación Física y el deporte

L'analisi delle pasioni: note a «La Regenta» di Clarín

Directory of Open Access Journals (Sweden)

Maria Rosso

2011-12-01

Full Text Available «La Regenta» es el fruto de las reflexiones teóricas de Clarín sobre el naturalismo, en las que el escritor reconoce un único dogma: «copiar la vida» en sus complejas relaciones humanas y sociales. Movido por el propósito de presentar un «cuadro general» de la realidad, en su novela compone un amplio retablo y analiza las pasiones de los personajes en sus recíprocas presiones conflictivas, que llevan a una implacable lucha por la autoafirmación, siguiendo un instinto primordial de conquistar, dominar y devorar. Entre las estrategias compositivas del autor, adquiere una particular eficacia la focalización múltiple, que utiliza las miradas de los personajes como importante instrumento narrativo y, al mismo tiempo, las convierte en un motivo esencial de la acción. Destaca, además, el arte del retrato, que explota las convenciones codificadas de la fisiognómica, integrándolas con una gestualidad comunicativa, en la que puede vislumbrarse la influencia de «La expresión de las emociones» de Darwin.

Antimicrobial Susceptibility and Genotypic Characteristic of Campylobacter spp. Isolates from Free-Living Birds in Poland.

Science.gov (United States)

Krawiec, Marta; Woźniak-Biel, Anna; Bednarski, Michał; Wieliczko, Alina

2017-11-01

Campylobacter spp. is the most commonly reported, bacterial cause of human foodborne infection worldwide. Commercial poultry and free-living birds are natural reservoirs of three particular species: Campylobacter jejuni, Campylobacter coli, and Campylobacter lari. The aim of this study was to determine the genotypic characteristics and antibiotic susceptibility of 43 Campylobacter strains, obtained from free-living birds, in Poland. In total, 700 birds were examined. The strains were isolated from 43 birds (6.14%) from the feces of 7 wild bird species: Mallard ducks Anas platyrhynchos (29 positive/121 tested), great cormorants Phalacrocorax carbo (5/77), velvet scoters Melanitta fusca (4/30), tawny owls Strix aluco (2/5), common buzzard Buteo buteo (1/3), rook Corvus frugilegus (1/6), and Eurasian tree sparrow Passer montanus (1/30). Thirty-eight (88.37%) of obtained strains belonged to C. jejuni and five (11.63%) to C. coli. Other 428 examined birds from different bird species were Campylobacter negative. The antimicrobial susceptibility to nine antimicrobials was also studied in investigated isolates of Campylobacter spp. Sixteen of the examined strains (37.21% of all positive samples) showed susceptibility to all of the nine antimicrobials. Moreover, the prevalence of selected virulence genes, such as flaA, cadF, ceuE, virB11, cdtA, cdtB, and cdtC were all analyzed. The virulence gene that was found most frequently in total number of Campylobacter strains was ceuE (72.10%) and other genes, such as flaA, cadF, cdtA, cdtB, and cdtC, were found in over 60% of all examined strains. Variable antimicrobial susceptibility and the presence of different virulence genes of examined strains, isolated from free-living birds, suggest that special attention should be given to wild birds and any potential approaches to the control of antibiotic-resistant Campylobacter should be discussed.

Computational prediction of secretion systems and secretomes of Brucella: identification of novel type IV effectors and their interaction with the host.

Science.gov (United States)

Sankarasubramanian, Jagadesan; Vishnu, Udayakumar S; Dinakaran, Vasudevan; Sridhar, Jayavel; Gunasekaran, Paramasamy; Rajendhran, Jeyaprakash

2016-01-01

Brucella spp. are facultative intracellular pathogens that cause brucellosis in various mammals including humans. Brucella survive inside the host cells by forming vacuoles and subverting host defence systems. This study was aimed to predict the secretion systems and the secretomes of Brucella spp. from 39 complete genome sequences available in the databases. Furthermore, an attempt was made to identify the type IV secretion effectors and their interactions with host proteins. We predicted the secretion systems of Brucella by the KEGG pathway and SecReT4. Brucella secretomes and type IV effectors (T4SEs) were predicted through genome-wide screening using JVirGel and S4TE, respectively. Protein-protein interactions of Brucella T4SEs with their hosts were analyzed by HPIDB 2.0. Genes coding for Sec and Tat pathways of secretion and type I (T1SS), type IV (T4SS) and type V (T5SS) secretion systems were identified and they are conserved in all the species of Brucella. In addition to the well-known VirB operon coding for the type IV secretion system (T4SS), we have identified the presence of additional genes showing homology with T4SS of other organisms. On the whole, 10.26 to 14.94% of total proteomes were found to be either secreted (secretome) or membrane associated (membrane proteome). Approximately, 1.7 to 3.0% of total proteomes were identified as type IV secretion effectors (T4SEs). Prediction of protein-protein interactions showed 29 and 36 host-pathogen specific interactions between Bos taurus (cattle)-B. abortus and Ovis aries (sheep)-B. melitensis, respectively. Functional characterization of the predicted T4SEs and their interactions with their respective hosts may reveal the secrets of host specificity of Brucella.

Divergent H7 immunogens offer protection from H7N9 virus challenge.

Science.gov (United States)

Krammer, Florian; Albrecht, Randy A; Tan, Gene S; Margine, Irina; Hai, Rong; Schmolke, Mirco; Runstadler, Jonathan; Andrews, Sarah F; Wilson, Patrick C; Cox, Rebecca J; Treanor, John J; García-Sastre, Adolfo; Palese, Peter

2014-04-01

The emergence of avian H7N9 viruses in humans in China has renewed concerns about influenza pandemics emerging from Asia. Vaccines are still the best countermeasure against emerging influenza virus infections, but the process from the identification of vaccine seed strains to the distribution of the final product can take several months. In the case of the 2009 H1N1 pandemic, a vaccine was not available before the first pandemic wave hit and therefore came too late to reduce influenza morbidity. H7 vaccines based on divergent isolates of the Eurasian and North American lineages have been tested in clinical trials, and seed strains and reagents are already available and can potentially be used initially to curtail influenza-induced disease until a more appropriately matched H7N9 vaccine is ready. In a challenge experiment in the mouse model, we assessed the efficacy of both inactivated virus and recombinant hemagglutinin vaccines made from seed strains that are divergent from H7N9 from each of the two major H7 lineages. Furthermore, we analyzed the cross-reactive responses of sera from human subjects vaccinated with heterologous North American and Eurasian lineage H7 vaccines to H7N9. Vaccinations with inactivated virus and recombinant hemagglutinin protein preparations from both lineages raised hemagglutination-inhibiting antibodies against H7N9 viruses and protected mice from stringent viral challenges. Similar cross-reactivity was observed in sera of human subjects from a clinical trial with a divergent H7 vaccine. Existing H7 vaccine candidates based on divergent strains could be used as a first line of defense against an H7N9 pandemic. In addition, this also suggests that H7N9 vaccines that are currently under development might be stockpiled and used for divergent avian H7 strains that emerge in the future. Sporadic human infections with H7N9 viruses started being reported in China in the early spring of 2013. Despite a significant drop in the number of

7 CFR 2902.7 - Determining biobased content.

Science.gov (United States)

2010-01-01

....7 Determining biobased content. (a) Certification requirements. For any product offered for... the weight (mass) of the total organic carbon in the material or product. (d) Products with the same...

Multivariate time series analysis of SafetyNet data. SafetyNet, Building the European Road Safety Observatory, Workpackage 7, Deliverable 7.7.

NARCIS (Netherlands)

Commandeur, J.J.F. Bijleveld, F.D. & Bergel, R.

2009-01-01

This deliverable provides an application of theories and methods documented in Deliverables 7.4 and 7.5 of work package 7 of the SafetyNet project. In this deliverable, use of select analysis techniques is demonstrated through real world road safety analysis problems, using aggregate data which may

Phase diagrams of systems of Sr2V2O7-M2V2O7 and of Ba2V2O7-M2V2O7 (M=Ca,Cd)

International Nuclear Information System (INIS)

Fotiev, A.A.; Zhuravlev, V.D.; Zhukov, V.P.

1982-01-01

Using the methods of X-ray phase and differential thermal anlyses phase equilibria in the systems Sr 2 V 2 O 7 -M 2 V 2 O 7 and Ba 2 V 2 O 7 -M 2 V 2 O 7 , where M--Ca, Cd, are studied, their phase diagrams being built. New double pyrovanadates Mosub(0.5)Srsub(1.5)Vsub(2)Osub(7) and MBaV 2 O 7 are found [ru

7 CFR 220.7 - Requirements for participation.

Science.gov (United States)

2010-01-01

... items such a grade, purchase units, style, condition, weight, ingredients, formulations, and delivery... citations affecting § 220.7, see the List of CFR Sections Affected, which appears in the Finding Aids...

7 CFR 773.7 - Loan uses.

Science.gov (United States)

2010-01-01

... PROGRAMS SPECIAL APPLE LOAN PROGRAM § 773.7 Loan uses. Loan funds may be used for any of the following purposes related to the production or marketing of apples: (a) Payment of costs associated with..., processing or marketing purposes; or (h) Payment of loan closing costs. ...

Metal-dependent regulation of ATP7A and ATP7B in fibroblast cultures

Directory of Open Access Journals (Sweden)

Lenartowicz Malgorzata

2016-08-01

Full Text Available Deficiency of one of the copper transporters ATP7A and ATP7B leads to the rare X-linked disorder Menkes Disease (MD or the rare autosomal disorder Wilson disease (WD, respectively. In order to investigate whether the ATP7A and the ATP7B genes may be transcriptionally regulated, we measured the expression level of the two genes at various concentrations of iron, copper and insulin. Treating fibroblasts from controls or from individuals with MD or WD for 3 and10 days with iron chelators revealed that iron deficiency led to increased transcript levels of both ATP7A and ATP7B. Copper deficiency obtained by treatment with the copper chelator led to a downregulation of ATP7A in the control fibroblasts, but surprisingly not in the WD fibroblasts. In contrast, the addition of copper led to an increased expression of ATP7A, but a decreased expression of ATP7B. Thus, whereas similar regulation patterns for the two genes were observed in response to iron deficiency, different responses were observed after changes in the access to copper. Mosaic fibroblast cultures from female carriers of MD treated with copper or copper chelator for 6-8 weeks led to clonal selection. Cells that express the normal ATP7A allele had a selective growth advantage at high copper concentrations, whereas more surprisingly, cells that express the mutant ATP7A allele had a selective growth advantage at low copper concentrations. Thus, although the transcription of ATP7A is regulated by copper, clonal growth selection in mosaic cell cultures is affected by the level of copper. Female carriers of MD are rarely affected probably due to a skewed inactivation of the X-chromosome bearing the ATP7A mutation.

Immune protection of microneme 7 (EmMIC7) against Eimeria maxima challenge in chickens.

Science.gov (United States)

Huang, Jingwei; Zhang, Zhenchao; Li, Menghui; Song, Xiaokai; Yan, Ruofeng; Xu, Lixin; Li, Xiangrui

2015-10-01

In the present study, the immune protective effects of recombinant microneme protein 7 of Eimeria maxima (rEmMIC7) and a DNA vaccine encoding this antigen (pVAX1-EmMIC7) on experimental challenge were evaluated. Two-week-old chickens were randomly divided into five groups. Experimental groups of chickens were immunized with 100 μg DNA vaccine pVAX1-MIC7 or 200 μg rEmMIC7, while control groups of chickens were injected with pVAX1 plasmid or sterile phosphate buffered saline (PBS). The results showed that the anti-EmMIC7 antibody titres in chickens of both rEmMIC7 and pVAX1-MIC7 groups were significantly higher as compared to PBS and pVAX1 control (P maxima challenge in chickens and it could be an effective antigen candidate for the development of new vaccines against E. maxima.

Mastering Windows 7 Deployment

CERN Document Server

Finn, Aidan; van Surksum, Kenneth

2011-01-01

Get professional-level instruction on Windows 7 deployment tools Enterprise-level operating system deployment is challenging and requires knowledge of specific tools. It is expected that Windows 7 will be extensively deployed in businesses worldwide. This comprehensive Sybex guide provides thorough coverage of the Microsoft deployment tools that were specifically created for Windows 7, preparing system administrators, MIS professionals, and corporate programmers to tackle the task effectively.Companies worldwide are expected to deploy Windows 7 as their enterprise operating system; system admi

Measurement of $^{7}$Be(n,$\\alpha$)$^{4}$He and $^{7}$Be(n,p)$^{7}$Li cross-sections for the Cosmological Lithium Problem

CERN Multimedia

We propose to measure in the second experimental area of n TOF the $^{7}$Be(n,$\\alpha$)$^{4}$He and $^{7}$Be(n,p)$^{7}$Li reaction in a wide energy range. Both reactions are of interest for the long- standing "Cosmological $^{7}$Li problem" in Big Bang Nucleosynthesis (BBN). The very high specific activity of $^{7}$Be, and the low cross section of the (n,$\\alpha$) reaction make this measurement extremely dicult. As afirst step, we request some beam time for detector tests at EAR2. For the $^{7}$Be(n,p) reaction, previously measured up to 13 keV, the diculty is mostly associated withthe availability of a high-purity $^{7}$Be sample. To this purpose we ask for three shifts of offline ISOLDE mass separation for the preparation of the sample to be used at n TOF. To this end, a prior endorsement by INTC of the scientific validity and feasibility of the proposed measurement is requested, to start activity on the sample production. The present proposal is part of a wider collaborative effort aimed at measuring neutr...

On the formation of 7-ketocholesterol from 7-dehydrocholesterol in patients with CTX and SLO

DEFF Research Database (Denmark)

Björkhem, Ingemar; Diczfalusy, Ulf; Lövgren-Sandblom, Anita

2014-01-01

with Smith-Lemli-Opitz syndrome (SLO) have markedly increased levels of 7-dehydrocholesterol in plasma and tissues. In addition, the former patients have markedly upregulated CYP7A1. We hypothesized that these patients may produce 7-ketocholesterol from 7-dehydrocholesterol with formation of cholesterol-7...

Differential gene expression by integrin β7+ and β7- memory T helper cells

Directory of Open Access Journals (Sweden)

Yang Yee

2004-07-01

Full Text Available Abstract Background The cell adhesion molecule integrin α4β7 helps direct the migration of blood lymphocytes to the intestine and associated lymphoid tissues. We hypothesized that β7+ and β7- blood memory T helper cells differ in their expression of genes that play a role in the adhesion or migration of T cells. Results RNA was prepared from β7+ and β7- CD4+ CD45RA- blood T cells from nine normal human subjects and analyzed using oligonucleotide microarrays. Of 21357 genes represented on the arrays, 16 were more highly expressed in β7+ cells and 18 were more highly expressed in β7- cells (≥1.5 fold difference and adjusted P + memory/effector T cells than on β7- cells. Conclusions Memory/effector T cells that express integrin β7 have a distinct pattern of expression of a set of gene transcripts. Several of these molecules can affect cell adhesion or chemotaxis and are therefore likely to modulate the complex multistep process that regulates trafficking of CD4+ memory T cell subsets with different homing behaviors.

Evaluation of live attenuated H7N3 and H7N7 vaccine viruses for their receptor binding preferences, immunogenicity in ferrets and cross reactivity to the novel H7N9 virus.

Directory of Open Access Journals (Sweden)

Qi Xu

Full Text Available Live attenuated influenza vaccine (LAIV candidates of the H7 subtype, A/Netherlands/219/03 (H7N7, NL03 ca and A/chicken/British Columbia/CN-6/2004 (H7N3, BC04 ca, were evaluated for their receptor binding specificity and immunogenicity in ferrets. The BC04 ca virus exhibited α2,3-SA and α2,6-SA dual receptor binding preference while the NL03 ca virus preferentially bound to α2,3-SA. Substitution of the Q226 and G228 (Q-G by the L226 and S228 (L-S residues in the HA improved binding to α2,6-SA for NL03 ca. The vaccine viruses with L-S retained the attenuation phenotype. NL03 L-S ca replicated more efficiently than the original NL03 ca virus in the upper respiratory tract of ferrets, and induced higher levels of humoral and cellular immune responses. Prior vaccination with seasonal LAIV reduced H7-specific antibody responses, but did not reduce the H7N7 vaccine mediated protection against a heterologous H7N3 BC04 wt virus infection in ferrets. In addition, the H7N3 and H7N7 vaccine immunized ferret sera cross reacted with the newly emerged H7N9 virus. These data, in combination with the safety data from previously conducted Phase 1 studies, suggest that these vaccines may have a role in responding to the threat posed by the H7N9 virus.

Java EE 7 handbook

CERN Document Server

Pilgrim, Peter A

2013-01-01

Java EE 7 Handbook is an example based tutorial with descriptions and explanations.""Java EE 7 Handbook"" is for the developer, designer, and architect aiming to get acquainted with the Java EE platform in its newest edition. This guide will enhance your knowledge about the Java EE 7 platform. Whether you are a long-term Java EE (J2EE) developer or an intermediate level engineer on the JVM with just Java SE behind you, this handbook is for you, the new contemporary Java EE 7 developer!

Mol 7C/6

International Nuclear Information System (INIS)

Aberle, J.; Schleisiek, K.; Schmuck, I.; Schmidt, L.; Romer, O.; Weih, G.

1995-01-01

The Mol 7C/6 coolant blockage experiment in the Belgian BR2 reactor yielded results different from Mol 7C experiments with low burnup pins: At 10% burnup local failure is not self-limiting, but requires active systems for detection and scram. The Mol 7C series was finished in 1991. In each of the test bundles Mol 7C/4, /5 and /6, 30 Mk I pins pre-irradiated in KNK II were used. The central blockage consisted of enriched UO 2 covering 30 percent of the bundle cross-section, with a height of 40 mm. The most important system for timely detection of coolant blockages of the type studied in Mol 7C/6 is based on DND. (orig.)

IL-7 Induces an Epitope Masking of γc Protein in IL-7 Receptor Signaling Complex

Directory of Open Access Journals (Sweden)

Tae Sik Goh

2017-01-01

Full Text Available IL-7 signaling via IL-7Rα and common γ-chain (γc is necessary for the development and homeostasis of T cells. Although the delicate mechanism in which IL-7Rα downregulation allows the homeostasis of T cell with limited IL-7 has been well known, the exact mechanism behind the interaction between IL-7Rα and γc in the absence or presence of IL-7 remains unclear. Additionally, we are still uncertain as to how only IL-7Rα is separately downregulated by the binding of IL-7 from the IL-7Rα/γc complex. We demonstrate here that 4G3, TUGm2, and 3E12 epitope masking of γc protein are induced in the presence of IL-7, indicating that the epitope alteration is induced by IL-7 binding to the preassembled receptor core. Moreover, the epitope masking of γc protein is inversely correlated with the expression of IL-7Rα upon IL-7 binding, implying that the structural alteration of γc might be involved in the regulation of IL-7Rα expression. The conformational change in γc upon IL-7 binding may contribute not only to forming the functional IL-7 signaling complex but also to optimally regulating the expression of IL-7Rα.

IL-7 Induces an Epitope Masking of γc Protein in IL-7 Receptor Signaling Complex

Science.gov (United States)

Goh, Tae Sik; Jo, Yuna; Lee, Byunghyuk; Kim, Geona; Hwang, Hyunju; Ko, Eunhee; Kang, Seung Wan; Oh, Sae-Ock; Baek, Sun-Yong; Yoon, Sik; Lee, Jung Sub

2017-01-01

IL-7 signaling via IL-7Rα and common γ-chain (γc) is necessary for the development and homeostasis of T cells. Although the delicate mechanism in which IL-7Rα downregulation allows the homeostasis of T cell with limited IL-7 has been well known, the exact mechanism behind the interaction between IL-7Rα and γc in the absence or presence of IL-7 remains unclear. Additionally, we are still uncertain as to how only IL-7Rα is separately downregulated by the binding of IL-7 from the IL-7Rα/γc complex. We demonstrate here that 4G3, TUGm2, and 3E12 epitope masking of γc protein are induced in the presence of IL-7, indicating that the epitope alteration is induced by IL-7 binding to the preassembled receptor core. Moreover, the epitope masking of γc protein is inversely correlated with the expression of IL-7Rα upon IL-7 binding, implying that the structural alteration of γc might be involved in the regulation of IL-7Rα expression. The conformational change in γc upon IL-7 binding may contribute not only to forming the functional IL-7 signaling complex but also to optimally regulating the expression of IL-7Rα. PMID:28127156

Straightforward synthesis of 2,2,4,4,5,7,7-d{sub 7}-cholestane: a new deuterated standard in petroleum analysis

Energy Technology Data Exchange (ETDEWEB)

Miranda, Maicon Guerra de; Albert, Andre Luis Mazzei; Cardoso, Jari Nobrega; Lopes, Rosangela Sabbatini Capella; Lopes, Claudio Cerqueira, E-mail: maicon_iq@yahoo.com [Universidade Federal do Rio de Janeiro (UFRJ), RJ (Brazil). Inst. de Quimica

2013-10-01

A short and efficient synthesis of heptadeuterated 2,2,4,4,5,7,7-d7-cholestane (1) from cholesterol (3) is described. The deuterated material will be useful for the analysis of different sources of petroleum in analytical geochemistry laboratories as internal standard for quantification of steranes via gas chromatography-mass spectrometry (GC-MS). (author)

39 CFR 7.1 - Definitions.

Science.gov (United States)

2010-07-01

... 39 Postal Service 1 2010-07-01 2010-07-01 false Definitions. 7.1 Section 7.1 Postal Service UNITED STATES POSTAL SERVICE THE BOARD OF GOVERNORS OF THE U.S. POSTAL SERVICE PUBLIC OBSERVATION (ARTICLE VII) § 7.1 Definitions. For purposes of §§ 7.2 through 7.8 of these bylaws: (a) The term Board means the...

7 CFR 1773.7 - Audit standards.

Science.gov (United States)

2010-01-01

... (CONTINUED) POLICY ON AUDITS OF RUS BORROWERS RUS Audit Requirements § 1773.7 Audit standards. (a) The audit must be performed in accordance with GAGAS and this part. The audit must be performed in accordance with GAGAS in effect at the audit date unless the borrower is directed otherwise, in writing, by RUS...

Trichloro(1,4,7-trimethyl-1,4,7-triazacyclononane)chromium(III)

DEFF Research Database (Denmark)

Klitgaard, Søren Kegnaes; Schau-Magnussen, Magnus

2005-01-01

The 1,4,7-trimethyl-1,4,7-triazacyclononane (tmtacn) ligand has become one of the classic ligands in coordination chemistry (Wieghardt et al., 1982 [Wieghardt, K., Chaudhuri, P., Nuber, B. & Weiss, J. (1982). Inorg. Chem. 21, 3086-3090.] ). In recent years, tmtacn-metal complexes ...

Regulator of G Protein Signaling 7 (RGS7) Can Exist in a Homo-oligomeric Form That Is Regulated by Gαo and R7-binding Protein.

Science.gov (United States)

Tayou, Junior; Wang, Qiang; Jang, Geeng-Fu; Pronin, Alexey N; Orlandi, Cesare; Martemyanov, Kirill A; Crabb, John W; Slepak, Vladlen Z

2016-04-22

RGS (regulator of G protein signaling) proteins of the R7 subfamily (RGS6, -7, -9, and -11) are highly expressed in neurons where they regulate many physiological processes. R7 RGS proteins contain several distinct domains and form obligatory dimers with the atypical Gβ subunit, Gβ5 They also interact with other proteins such as R7-binding protein, R9-anchoring protein, and the orphan receptors GPR158 and GPR179. These interactions facilitate plasma membrane targeting and stability of R7 proteins and modulate their activity. Here, we investigated RGS7 complexes using in situ chemical cross-linking. We found that in mouse brain and transfected cells cross-linking causes formation of distinct RGS7 complexes. One of the products had the apparent molecular mass of ∼150 kDa on SDS-PAGE and did not contain Gβ5 Mass spectrometry analysis showed no other proteins to be present within the 150-kDa complex in the amount close to stoichiometric with RGS7. This finding suggested that RGS7 could form a homo-oligomer. Indeed, co-immunoprecipitation of differentially tagged RGS7 constructs, with or without chemical cross-linking, demonstrated RGS7 self-association. RGS7-RGS7 interaction required the DEP domain but not the RGS and DHEX domains or the Gβ5 subunit. Using transfected cells and knock-out mice, we demonstrated that R7-binding protein had a strong inhibitory effect on homo-oligomerization of RGS7. In contrast, our data indicated that GPR158 could bind to the RGS7 homo-oligomer without causing its dissociation. Co-expression of constitutively active Gαo prevented the RGS7-RGS7 interaction. These results reveal the existence of RGS protein homo-oligomers and show regulation of their assembly by R7 RGS-binding partners. © 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

7-Glutathione-pyrrole and 7-cysteine-pyrrole are potential carcinogenic metabolites of pyrrolizidine alkaloids.

Science.gov (United States)

He, Xiaobo; Xia, Qingsu; Fu, Peter P

2017-04-03

Many pyrrolizidine alkaloids (PAs) are hepatotoxic, genotoxic, and carcinogenic phytochemicals. Metabolism of PAs in vivo generates four (±)-6,7-dihydro-7-hydroxy-1-hydroxymethyl-5H-pyrrolizine (DHP)-DNA adducts that have been proposed to be responsible for PA-induced liver tumor formation in rats. In this present study, we determined that the same set of DHP-DNA adducts was formed upon the incubation of 7-glutathione-DHP and 7-cysteine-DHP with cultured human hepatocarcinoma HepG2 cells. These results suggest that 7-glutathione-DHP and 7-cysteine-DHP are reactive metabolites of PAs that can bind to cellular DNA to form DHP-DNA adducts in HepG2 cells, and can potentially initiate liver tumor formation.

7 CFR 1124.7 - Pool plant.

Science.gov (United States)

2010-01-01

... agreement for other than Class I use) into ultra-pasteurized or aseptically-processed fluid milk products...) located within one or more States that require handlers to pay minimum prices for raw milk provided that... prices for raw milk. Subject to the following exclusions: (i) The plant is described in § 1124.7(a) or (b...

7 CFR 8.7 - Continued use.

Science.gov (United States)

2010-01-01

... the Secretary of Agriculture 4-H CLUB NAME AND EMBLEM § 8.7 Continued use. (a) The Cooperative... use the 4-H Club Name or Emblem: (1) For their own educational or informational purposes according to... materials bearing the 4-H Club Name or Emblem. (4) Any proposal for distribution on an interstate, regional...

12 CFR 1291.7 - Monitoring.

Science.gov (United States)

2010-01-01

... 12 Banks and Banking 7 2010-01-01 2010-01-01 false Monitoring. 1291.7 Section 1291.7 Banks and... HOUSING PROGRAM § 1291.7 Monitoring. (a) Competitive application program—(1) Initial monitoring policies... connection with the project. (ii) Back-up and other project documentation. The Bank's written monitoring...

Importance of the tensor interaction in the (/sup 7/Li, /sup 7/Be) reaction

Energy Technology Data Exchange (ETDEWEB)

Dodd, A.C.; Clarke, N.M.; Coopersmith, J.; Griffiths, R.J.; Pearce, K.I.; Stanley, B.; Cook, J.

1985-09-01

Data for the /sup 28/Si(/sup 7/Li, /sup 7/Be)/sup 28/Al reaction at 72 MeV and for the /sup 26/Mg(/sup 7/Li, /sup 7/Be)/sup 26/Na reaction at 88 MeV are presented together with one-step DWBA calculations using microscopic form factors. The tensor interaction is shown to be important to explain the structureless nature of the angular distributions.

Trealose e trealase em tenebrio molitor L Trehalose and Trehalase in Tenebrio molitor L

Directory of Open Access Journals (Sweden)

Cristina Piedras Lopes

1972-01-01

Full Text Available Foi estudada a concentração em trealose e a atividade em trealase do Tenebrio molitor L. durante as tres fases da metamorfose (larva, ninfa, imago. Verificou-se que na larva e no adulto os valores são mais elevados conforme a curva da fig. 3. A trealose foi expressa em mg/g de Tenebrio e a trealose por µg de glicose/mg proteina.Trehalose and trehalase were determined in the Tenebrio molitor L., using larva, pupa and imago. A total number of 895 animals was analyzed. A growth curve up to the end of the larval stage was established (Fig. 1 and compared with the normal one obtained by Fraenkel. It was shown that trehalose and trehalase are more concentrated in the larva and imago presenting a curve with two arms as depicted in the Fig. 3. Trehalase was expressed in mg/g of Tenebrio and trehalase in µg of glucose /mg proteín.

IGFBP-4 Fragments as Markers of Cardiovascular Mortality in Type 1 Diabetes Patients With and Without Nephropathy

DEFF Research Database (Denmark)

Hjortebjerg, Rikke; Tarnow, Lise; Jorsal, Anders

2015-01-01

CONTEXT: Type 1 diabetes (T1D) is characterized by an increased risk of macrovascular complications. Pregnancy-associated plasma protein-A (PAPP-A) generated N- and C-terminal fragments of IGF binding protein-4 (NT-IGFBP-4 and CT-IGFBP-4) have been suggested as cardiac biomarkers. OBJECTIVE......: The objective of the study was to investigate the prognostic value of IGFBP-4 fragments in a cohort of T1D patients. DESIGN AND PATIENTS: We prospectively followed up 178 T1D patients with diabetic nephropathy and 152 T1D patients with normoalbuminuria for 12.6 (range 0.2-12.9) years. MAIN OUTCOME MEASURES...... of cardiovascular mortality with hazard ratios [95% confidence interval (CI)] of 5.81 (95% CI 2.62-12.86) (P 1.10-6.10) (P = .030), respectively. After adjustments, PAPP-A was not associated with overall or cardiovascular death. All IGF protein levels were higher in patients with diabetic...

PAPP-A proteolytic activity enhances IGF bioactivity in ascites from women with ovarian carcinoma

Science.gov (United States)

Thomsen, Jacob; Hjortebjerg, Rikke; Espelund, Ulrick; Ørtoft, Gitte; Vestergaard, Poul; Magnusson, Nils E.; Conover, Cheryl A.; Tramm, Trine; Hager, Henrik; Høgdall, Claus; Høgdall, Estrid; Oxvig, Claus; Frystyk, Jan

2015-01-01

Pregnancy-associated plasma protein-A (PAPP-A) stimulates insulin-like growth factor (IGF) action through proteolysis of IGF-binding protein (IGFBP)-4. In experimental animals, PAPP-A accelerates ovarian tumor growth by this mechanism. To investigate the effect of PAPP-A in humans, we compared serum and ascites from 22 women with ovarian carcinoma. We found that ascites contained 46-fold higher PAPP-A levels as compared to serum (P IGF-I receptor (IGF-IR) in vitro (+31%, P IGF-I, and lower levels of IGF-II (P IGF-IR in all but one tumor, whereas all tumors expressed PAPP-A, IGFBP-4, IGF-I and IGF-II. Addition of recombinant PAPP-A to ascites increased the cleavage of IGFBP-4 and enhanced IGF-IR activation (P IGFs and these proteins are also present in ascites. We suggest that both soluble PAPP-A in ascites and tissue-associated PAPP-A serve to increase IGF bioactivity and, thereby, to stimulate IGF-IR-mediated tumor growth. PMID:26336825

12 CFR 944.7 - Reports.

Science.gov (United States)

2010-01-01

... 12 Banks and Banking 7 2010-01-01 2010-01-01 false Reports. 944.7 Section 944.7 Banks and Banking FEDERAL HOUSING FINANCE BOARD FEDERAL HOME LOAN BANK MISSION COMMUNITY SUPPORT REQUIREMENTS § 944.7 Reports. Each Advisory Council annual report required to be submitted to the Finance Board pursuant to...

50 CFR 520.7 - Fees.

Science.gov (United States)

2010-10-01

... 50 Wildlife and Fisheries 7 2010-10-01 2010-10-01 false Fees. 520.7 Section 520.7 Wildlife and Fisheries MARINE MAMMAL COMMISSION PUBLIC AVAILABILITY OF AGENCY MATERIALS § 520.7 Fees. (a) The following... Counsel or the Director determines that waiver or reduction of the fee is in the public interest because...

12 CFR 950.7 - Collateral.

Science.gov (United States)

2010-01-01

... 12 Banks and Banking 7 2010-01-01 2010-01-01 false Collateral. 950.7 Section 950.7 Banks and... Advances to Members § 950.7 Collateral. (a) Eligible security for advances to all members. At the time of... interest in collateral that meets the requirements of one or more of the following categories: (1) Mortgage...

7 CFR 301.45-7 - Assembly and inspection of regulated articles and outdoor household articles.

Science.gov (United States)

2010-01-01

... 7 Agriculture 5 2010-01-01 2010-01-01 false Assembly and inspection of regulated articles and outdoor household articles. 301.45-7 Section 301.45-7 Agriculture Regulations of the Department of... QUARANTINE NOTICES Gypsy Moth § 301.45-7 Assembly and inspection of regulated articles and outdoor household...

7 CFR 215.7 - Requirements for participation.

Science.gov (United States)

2010-01-01

... authority under this part to support a nonprofit nutrition program for the elderly, including a program... Agriculture Regulations of the Department of Agriculture (Continued) FOOD AND NUTRITION SERVICE, DEPARTMENT OF AGRICULTURE CHILD NUTRITION PROGRAMS SPECIAL MILK PROGRAM FOR CHILDREN § 215.7 Requirements for participation...

Proteinas do liquido cefalorraqueano: III. Valores normais das imuneglobulinas G, A e M (variações ligadas a cor, sexo e idade

Directory of Open Access Journals (Sweden)

Lucia M. Singer Vermes

1983-03-01

Full Text Available É apresentada inicialmente revisão de literatura a respeito dos resultados obtidos na determinação das taxas de imuneglobulinas do LCR normal, mediante diferentes métodos, assim como da influência dos fatores cor, sexo e idade, nestes valores. Mediante imunodifusão radial foram determinados os níveis de IgG, IgA e IgM do LCR normal de 115, 78 e 45 pessoas, respectivamente. Todas as amostras de LCR foram obtidas por punção sub-occipital (LCR-SO. Os resultados destas determinações permitiram concluir que: a as taxas médias de IgG, IgA e IgM do LCR de pretos ou pardos não diferem daquelas de brancos; b não existem variações ligadas ao sexo, nem à idade, em crianças com 1 a 11 anos, nas taxas das imuneglobulinas estudadas, no LCR; c os níveis fisiológicos em crianças com 1 a 11 anos, para IgG variam entre 0,21 e 2,93 mg/100 ml; os teores absolutos médios de IgG de crianças são inferiores àqueles encontrados em adultos; os níveis percentuais médios de IgG de crianças não diferem daqueles de adultos; d as taxas de IgA do LCR de crianças, tanto em valores absolutos como relativos, são, em média, inferiores àquelas encontradas em adultos, não ultrapassando, em condições normais, 0,15 mg/100 ml (0,7% do total protéico; e em adultos, não se observam variações ligadas ao sexo, nem à idade, nos níveis das imuneglobulinas estudadas, no LCR-SO; as taxas normais de IgG encontradas no LCR-SO de adultos são de 0,51 a 4,00 mg/100 ml; em crianças e adultos, as taxas fisiológicas desta imuneglobulina, em termos percentuais, são de 3 a 12%; o LCR-SO normal de adultos contém IgA em concentrações de até 0,32 mg/100 ml (0,9% do total protéico; f a IgM encontra-se no LCR-SO normal, tanto de crianças como de adultos, em níveis muito baixos, não ultrapassando 0,2 mg/100 ml (0,25% do total protéico.

Adsorption of carbon monoxide on the Si(111)-7 × 7 surface

Energy Technology Data Exchange (ETDEWEB)

Shong, Bonggeun, E-mail: bshong@cnu.ac.kr

2017-05-31

Highlights: • Detailed chemistry of CO with the Si(111)-7 × 7 surface is computationally studied. • On-top on rest-atoms and back-bond insertion on adatoms are suggested geometries. • The two structures exhibit no activation barrier for adsorption and significant stability. • Geometrical and spectroscopic properties of CO adsorbates are predicted. • Direction of the interfacial charge transfer depends on the bonding configuration. - Abstract: The adsorption of CO and surface chemistry of Si are well-understood topics in surface science. However, research into the adsorption of CO on the Si(111)-7 × 7 surface is deficient. In this study, the adsorption of CO on Si(111)-7 × 7 is investigated via high-level density functional theory calculations using cluster model. Two adsorption configurations are found to be kinetically and thermodynamically viable: on-top on rest-atoms and back-bond insertion on adatoms, both binding to the surface via C atom. Structural, electronic, and spectroscopic properties of the adsorbates indicate a σ-donating/π-accepting nature of the CO−Si bonds in both configurations. The domination of σ-donation in the on-top configuration results in a net positive charge on the on-top adsorbate, and the opposite situation yields a net negative charge on the back-bond insertion adsorbates. Our study provides a detailed understanding of the previous experimental observations of fundamental surface chemical phenomena, suggesting possible applications of Si surface functionalization using CO.

Função renal de pacientes de unidade de terapia intensiva: creatinina plasmática e proteína carreadora do retinol urinário Renal function of intensive care unit patients: plasma creatinine and urinary retinol-binding protein

Directory of Open Access Journals (Sweden)

Cristina Satoko Mizoi

2008-12-01

Full Text Available OBJETIVOS: A avaliação precoce da disfunção renal usando marcadores usuais não supre uma indicação quer da sensitividade e da especificidade da disfunção renal de pacientes críticos. Seriam desejáveis marcadores mais específicos e sensíveis para a detecção precoce de um processo fisiopatológico renal em fase inicial. A proteína carreadora do retinol urinário poderia ser um método alternativo para avaliação precoce da função renal destes pacientes. MÉTODOS: O estudo acompanhou 100 pacientes em terapia intensiva e avaliou suas variáveis clinicas e laboratoriais, incluindo a dosagem de creatinina plasmática e proteina carreadora do retinol urinário e as variáveis demográficas. RESULTADOS: A amostra foi caracterizada por pacientes geriátricos (63,4±15,6 anos, homens (68%, sendo 53% cirúrgicos. Análise estatística mostrou associação entre creatinina plasmática e as seguintes variáveis: gênero (p=0,026, idade (p=0,038, uso de medicação vasoativa (p=0,003, proteinúria (p=0,025, escore Acute Physiological Chronic Health Evaluation (APACHE II (p=0,000, uréia (p=0,000, potássio (p=0,003 clearance de creatinina estimado (p=0,000. A proteína carreadora do retinol urinário correlacionava-se com outras variáveis: peso usa de ventilação invasiva (p=0,000, uso de medicamentos antinfamatórios não-esteróides (p=0,018, uso de medicação vasoativa (p=0,021, temperatura alta (>37,5ºC (p=0,005, proteinúria (p=0,000, bilirubinúria (p=0,004, fluxo urinário (p=0,019, pressão diastólica mínima (p=0,032, pressão sistólica mínima (p=0,029, APACHE II (p=0.000, creatinina (p=0,001, uréia (p=0,001 e clearance de creatinina estimado (p=0,000. A proteína carreadora do retinol urinário também tende a ser associada com doença renal anterior, vasculopatias e neoplasias. Na análise univariada, a fração de excreção de sódio se correlacionou com creatinina plasmática e proteina carreadora do retinol urin

RELAP-7 Closure Correlations

Energy Technology Data Exchange (ETDEWEB)

Zou, Ling [Idaho National Lab. (INL), Idaho Falls, ID (United States); Berry, R. A. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Martineau, R. C. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Andrs, D. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Zhang, H. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Hansel, J. E. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Sharpe, J. P. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Johns, Russell C. [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

2017-04-01

The RELAP-7 code is the next generation nuclear reactor system safety analysis code being developed at the Idaho National Laboratory (INL). The code is based on the INL’s modern scientific software development framework, MOOSE (Multi-Physics Object Oriented Simulation Environment). The overall design goal of RELAP-7 is to take advantage of the previous thirty years of advancements in computer architecture, software design, numerical integration methods, and physical models. The end result will be a reactor systems analysis capability that retains and improves upon RELAP5’s and TRACE’s capabilities and extends their analysis capabilities for all reactor system simulation scenarios. The RELAP-7 code utilizes the well-posed 7-equation two-phase flow model for compressible two-phase flow. Closure models used in the TRACE code has been reviewed and selected to reflect the progress made during the past decades and provide a basis for the colure correlations implemented in the RELAP-7 code. This document provides a summary on the closure correlations that are currently implemented in the RELAP-7 code. The closure correlations include sub-grid models that describe interactions between the fluids and the flow channel, and interactions between the two phases.

Effect of passive smoking on the levels of pregnancy associated plasma protein-A in normal rats

International Nuclear Information System (INIS)

Naveed, A.K.; Rahim, A.; Malik, M.S.

2010-01-01

To measure the levels of pregnancy associated plasma protein- A (PAPPA-A) in normal rats exposed to cigarette smoke. Sixty albino rats of Sprague- Dawley strain weighing 200-250 gm, divided into two groups. Both the groups were kept in identical chambers. One group of 30 rats was further exposed to passive cigarette smoke for 4 weeks. No increase was observed in the levels of serum PAPP-A of both the groups: Passive smokers and not exposed to passive smoking i.e. P > 0.05. Smoking does not increase the levels of PAPP-A. (author)

PROTEINA BLANCO DE LOS AUTO-ANTICUERPOS ANTI-P ASOCIADOS A LUPUS NEUROPSIQUATRICO: FUNCION Y MECANISMOS PATOGENICOS

OpenAIRE

segovia miranda, fabian josue

2012-01-01

El potencial patogénico de los auto-anticuerpos en las disfunciones del sistema nervioso central ha atraído gran interés en los últimos años. La identificación de autoanticuerpos neuropatogénicos dirigidos contra componentes de la superficie neuronal ayuda a entender la influencia que el sistema inmune puede ejercer sobre procesos que involucran memoria, cognición y comportamiento. También provee herramientas para entender mejor la función de componentes ya conocidos, o de nuevos com...

Search for a resonant enhancement of the 7Be + d reaction and primordial 7Li abundances

International Nuclear Information System (INIS)

O'Malley, Patrick; Adekola, A.S.; Cizewski, J.A.; Howard, M.E.; Strauss, S.; Bardayan, Daniel W.; Chae, Kyung Yuk; Nesaraja, Caroline D.; Pain, Steven D.; Smith, Michael Scott; Ahn, S.H.; Jones, K.L.; Pittman, S.T.; Schmitt, Kyle; Graves, S.; Kozub, R.L.; Shriner, J.F. Jr.; Wheeler, J.L.; Linhardt, Laura; Matos, M.; Moazen, B.M.; Peters, W.A.; Spassova, I.

2011-01-01

Big Bang nucleosynthesis calculations, constrained by the Wilkinson Microwave Anisotropy Probe results, produce 7 Li abundances almost a factor of four larger than those extrapolated from observations. Since primordial 7 Li is believed to be mostly produced by the beta decay of 7 Be, one proposed solution to this discrepancy is a resonant enhancement of the 7 Be(d,p)2α reaction rate through the 5/2 + 16.7-MeV state in 9 B. The 2 H( 7 Be,d) 7 Be reaction was used to search for such a resonance; none was observed. An upper limit on the width of the proposed resonance was deduced.

HPV16-E7-Specific Activated CD8 T Cells in E7 Transgenic Skin and Skin Grafts

Directory of Open Access Journals (Sweden)

Seyed Davoud Jazayeri

2017-05-01

Full Text Available Human papillomavirus (HPV 16 E7 (E7 protein expression in skin promotes epithelial hyperproliferation and transformation to malignancy. Grafts of murine skin expressing E7 protein as a transgene in keratinocytes are not rejected from immunocompetent recipients, whereas grafts expressing ovalbumin (OVA, with or without coexpression of E7 protein, are promptly rejected, demonstrating that E7-associated non-antigen-specific local immunosuppression is not a major determinant of lack of rejection of E7 transgenic skin. To determine whether failure of rejection of E7 skin grafts is due to failure to attract E7-specific effector T cells, E7- and OVA-specific effector CD8+ T cells, activated in vitro, were transferred to animals bearing E7 transgenic skin grafts. Three days after T cell transfer, E7-specific T cells were present in significantly greater numbers than OVA-specific T cells in the grafted skin on animals bearing recently placed or healed E7 grafts, without graft rejection, and also in the ear skin of E7 transgenic animals, without obvious pathology. E7 and OVA-specific T cells were present in lesser numbers in healed E7 grafts than in recently placed grafts and in lesser numbers in recently placed E7 transgenic epidermal grafts without E7-associated hyperproliferation, derived from E7 transgenic mice with a mutated retinoblastoma gene. These data demonstrate that effector T cells are to some extent attracted to E7 transgenic skin specifically by E7 expression, but in large measure non-specifically by the epithelial proliferation associated with E7 expression, and by the local inflammation produced by grafting. Failure of E7 graft rejection was observed despite trafficking of E7-specific effector T cells to E7-expressing epithelium, a finding of consequence for immunotherapy of HPV 16 E7-associated human cancers.

Performance comparison of two androgen receptor splice variant 7 (AR-V7) detection methods.

Science.gov (United States)

Bernemann, Christof; Steinestel, Julie; Humberg, Verena; Bögemann, Martin; Schrader, Andres Jan; Lennerz, Jochen K

2018-01-23

To compare the performance of two established androgen receptor splice variant 7 (AR-V7) mRNA detection systems, as paradoxical responses to next-generation androgen-deprivation therapy in AR-V7 mRNA-positive circulating tumour cells (CTC) of patients with castration-resistant prostate cancer (CRPC) could be related to false-positive classification using detection systems with different sensitivities. We compared the performance of two established mRNA-based AR-V7 detection technologies using either SYBR Green or TaqMan chemistries. We assessed in vitro performance using eight genitourinary cancer cell lines and serial dilutions in three AR-V7-positive prostate cancer cell lines, as well as in 32 blood samples from patients with CRPC. Both assays performed identically in the cell lines and serial dilutions showed identical diagnostic thresholds. Performance comparison in 32 clinical patient samples showed perfect concordance between the assays. In particular, both assays determined AR-V7 mRNA-positive CTCs in three patients with unexpected responses to next-generation anti-androgen therapy. Thus, technical differences between the assays can be excluded as the underlying reason for the unexpected responses to next-generation anti-androgen therapy in a subset of AR-V7 patients. Irrespective of the method used, patients with AR-V7 mRNA-positive CRPC should not be systematically precluded from an otherwise safe treatment option. © 2018 The Authors BJU International © 2018 BJU International Published by John Wiley & Sons Ltd.

The chimeric gene CHRFAM7A, a partial duplication of the CHRNA7 gene, is a dominant negative regulator of α7*nAChR function.

Science.gov (United States)

Araud, Tanguy; Graw, Sharon; Berger, Ralph; Lee, Michael; Neveu, Estele; Bertrand, Daniel; Leonard, Sherry

2011-10-15

The human α7 neuronal nicotinic acetylcholine receptor gene (CHRNA7) is a candidate gene for schizophrenia and an important drug target for cognitive deficits in the disorder. Activation of the α7*nAChR, results in opening of the channel and entry of mono- and divalent cations, including Ca(2+), that presynaptically participates to neurotransmitter release and postsynaptically to down-stream changes in gene expression. Schizophrenic patients have low levels of α7*nAChR, as measured by binding of the ligand [(125)I]-α-bungarotoxin (I-BTX). The structure of the gene, CHRNA7, is complex. During evolution, CHRNA7 was partially duplicated as a chimeric gene (CHRFAM7A), which is expressed in the human brain and elsewhere in the body. The association between a 2bp deletion in CHRFAM7A and schizophrenia suggested that this duplicate gene might contribute to cognitive impairment. To examine the putative contribution of CHRFAM7A on receptor function, co-expression of α7 and the duplicate genes was carried out in cell lines and Xenopus oocytes. Expression of the duplicate alone yielded protein expression but no functional receptor and co-expression with α7 caused a significant reduction of the amplitude of the ACh-evoked currents. Reduced current amplitude was not correlated with a reduction of I-BTX binding, suggesting the presence of non-functional (ACh-silent) receptors. This hypothesis is supported by a larger increase of the ACh-evoked current by the allosteric modulator 1-(5-chloro-2,4-dimethoxy-phenyl)-3-(5-methyl-isoxazol-3-yl)-urea (PNU-120596) in cells expressing the duplicate than in the control. These results suggest that CHRFAM7A acts as a dominant negative modulator of CHRNA7 function and is critical for receptor regulation in humans. Copyright © 2011 Elsevier Inc. All rights reserved.

Crystal structure of tetraaquabis(1,3-dimethyl-2,6-dioxo-7H-purin-7-ido-κN7cobalt(II

Directory of Open Access Journals (Sweden)

Hicham El Hamdani

2017-09-01

Full Text Available The title complex, [Co(C7H7N4O22(H2O4], comprises mononuclear molecules consisting of a CoII ion, two deprotonated theophylline ligands (systematic name: 1,3-dimethyl-7H-purine-2,6-dione and four coordinating water molecules. The CoII atom lies on an inversion centre and has a slightly distorted octahedral coordination environment, with two N atoms of two trans-oriented theophylline ligands and the O atoms of four water molecules. An intramolecular hydrogen bond stabilizes this conformation. A three-dimensional supramolecular network structure is formed by intermolecular O—H...O and O—H...N hydrogen bonds.

CYP7B1

DEFF Research Database (Denmark)

Roos, P; Svenstrup, K; Danielsen, E R

2014-01-01

UNLABELLED: The SPG5A subtype of Hereditary Spastic Paraplegia (HSP) is a rare autosomal recessive neurodegenerative disorder caused by mutations in the CYP7B1 gene, which encodes a steroid cytochrome P450 7α-hydroxylase. This enzyme provides the primary metabolic route for neurosteroids. Clinica......UNLABELLED: The SPG5A subtype of Hereditary Spastic Paraplegia (HSP) is a rare autosomal recessive neurodegenerative disorder caused by mutations in the CYP7B1 gene, which encodes a steroid cytochrome P450 7α-hydroxylase. This enzyme provides the primary metabolic route for neurosteroids.......945_947 dupGGC p.A316AA). CONCLUSION: SPG5A could be characterized as a predominantly pure HSP. MRS showing elevated mI/Cr ratio in the white matter may be indicative of SPG5A....

Microglial-derived miRNA let-7 and HMGB1 contribute to ethanol-induced neurotoxicity via TLR7.

Science.gov (United States)

Coleman, Leon G; Zou, Jian; Crews, Fulton T

2017-01-25

Toll-like receptor (TLR) signaling is emerging as an important component of neurodegeneration. TLR7 senses viral RNA and certain endogenous miRNAs to initiate innate immune responses leading to neurodegeneration. Alcoholism is associated with hippocampal degeneration, with preclinical studies linking ethanol-induced neurodegeneration with central innate immune induction and TLR activation. The endogenous miRNA let-7b binds TLR7 to cause neurodegeneration. TLR7 and other immune markers were assessed in postmortem human hippocampal tissue that was obtained from the New South Wales Tissue Bank. Rat hippocampal-entorhinal cortex (HEC) slice culture was used to assess specific effects of ethanol on TLR7, let-7b, and microvesicles. We report here that hippocampal tissue from postmortem human alcoholic brains shows increased expression of TLR7 and increased microglial activation. Using HEC slice culture, we found that ethanol induces TLR7 and let-7b expression. Ethanol caused TLR7-associated neuroimmune gene induction and initiated the release let-7b in microvesicles (MVs), enhancing TLR7-mediated neurotoxicity. Further, ethanol increased let-7b binding to the danger signaling molecule high mobility group box-1 (HMGB1) in MVs, while reducing let-7 binding to classical chaperone protein argonaute (Ago2). Flow cytometric analysis of MVs from HEC media and analysis of MVs from brain cell culture lines found that microglia were the primary source of let-7b and HMGB1-containing MVs. Our results identify that ethanol induces neuroimmune pathology involving the release of let-7b/HMGB1 complexes in microglia-derived microvesicles. This contributes to hippocampal neurodegeneration and may play a role in the pathology of alcoholism.

7 CFR 322.7 - Notice of arrival.

Science.gov (United States)

2010-01-01

... Honeybees, Honeybee Germ Plasm, and Bees Other Than Honeybees From Approved Regions § 322.7 Notice of... or honeybee germ plasm imported into the United States under this subpart, you must notify APHIS of...; or (2) By facsimile at (301) 734-8700; or (3) By electronic mail to [email protected] (Approved...

Windows 7 resource kit

CERN Document Server

Northrup, Tony; Honeycutt, Jerry; Wilson, Ed

2009-01-01

In-depth and comprehensive, this RESOURCE KIT delivers the information you need to administer your Windows 7 system. You get authoritative technical guidance from those who know the technology best-Microsoft Most Valuable Professionals (MVPs) and the Windows 7 product team-along with essential scripts and resources. In addition, "Direct from the Source" sidebars offer deep insights and troubleshooting tips from the Windows 7 team. Get expert guidance on how to: Use Microsoft Deployment Toolkit best practices and tools. Plan user-state migration and test application compatibility.

Easy QC 7 tools

International Nuclear Information System (INIS)

1981-04-01

This book explains method of QC 7 tools, mind for using QC 7 tools, effect of QC 7 tools application, giving descriptions of graph, pareto's diagram like application writing way and using method of pareto's diagram, characteristic diagram, check sheet such as purpose and subject of check, goals and types of check sheet, and using point of check sheet, histogram like application and using method, and stratification, scatterplot, control chart, promotion method and improvement and cases of practice of QC tools.

Easy QC 7 tools

Energy Technology Data Exchange (ETDEWEB)

NONE

1981-04-15

This book explains method of QC 7 tools, mind for using QC 7 tools, effect of QC 7 tools application, giving descriptions of graph, pareto's diagram like application writing way and using method of pareto's diagram, characteristic diagram, check sheet such as purpose and subject of check, goals and types of check sheet, and using point of check sheet, histogram like application and using method, and stratification, scatterplot, control chart, promotion method and improvement and cases of practice of QC tools.

22 CFR 911.7 - Review.

Science.gov (United States)

2010-04-01

... 22 Foreign Relations 2 2010-04-01 2010-04-01 true Review. 911.7 Section 911.7 Foreign Relations FOREIGN SERVICE GRIEVANCE BOARD IMPLEMENTATION DISPUTES § 911.7 Review. Resolution of disputes under this section shall not be subject to judicial review. ...

14 CFR 413.7 - Application.

Science.gov (United States)

2010-01-01

... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Application. 413.7 Section 413.7 Aeronautics and Space COMMERCIAL SPACE TRANSPORTATION, FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION LICENSING LICENSE APPLICATION PROCEDURES § 413.7 Application. (a) Form. An application must be in...

Apache Tomcat 7 Essentials

CERN Document Server

Khare, Tanuj

2012-01-01

This book is a step-by-step tutorial for anyone wanting to learn Apache Tomcat 7 from scratch. There are plenty of illustrations and examples to escalate you from a novice to an expert with minimal strain. If you are a J2EE administrator, migration administrator, technical architect, or a project manager for a web hosting domain, and are interested in Apache Tomcat 7, then this book is for you. If you are someone responsible for installation, configuration, and management of Tomcat 7, then too, this book will be of help to you.

27 CFR 7.1 - General.

Science.gov (United States)

2010-04-01

... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false General. 7.1 Section 7.1... TREASURY LIQUORS LABELING AND ADVERTISING OF MALT BEVERAGES Scope § 7.1 General. The regulations in this part relate to the labeling and advertising of malt beverages. ...

12 CFR 564.7 - Enforcement.

Science.gov (United States)

2010-01-01

... 12 Banks and Banking 5 2010-01-01 2010-01-01 false Enforcement. 564.7 Section 564.7 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY APPRAISALS § 564.7 Enforcement. Institutions and institution-affiliated parties, including staff appraisers and fee appraisers, who violate...

44 CFR 10.7 - Planning.

Science.gov (United States)

2010-10-01

... 44 Emergency Management and Assistance 1 2010-10-01 2010-10-01 false Planning. 10.7 Section 10.7... GENERAL ENVIRONMENTAL CONSIDERATIONS Agency Implementing Procedures § 10.7 Planning. (a) Early planning. The Regional Administrator shall integrate the NEPA process with other planning at the earliest...

31 CFR 281.7 - Limitations.

Science.gov (United States)

2010-07-01

... 31 Money and Finance: Treasury 2 2010-07-01 2010-07-01 false Limitations. 281.7 Section 281.7 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) FISCAL SERVICE, DEPARTMENT OF THE TREASURY FINANCIAL MANAGEMENT SERVICE FOREIGN EXCHANGE OPERATIONS § 281.7 Limitations. The...

Synthesis of 3-[D7]isopropylbenzo-2-thia-1,3-diazinon-(4)-2,2- dioxide ([D7]-bentazone)

NARCIS (Netherlands)

Jacquemijns M; Zomer G

1990-01-01

D7]Bentazone was sysnthesized from methyl antranilate and [D7 isopropylsulfamoyl chloride. [D7]Isopropylsulfamoyl chloride was prepared by treatment of [D7]isopropyl amine with chlorosulfonic acid and phosphorous pentachloride. The overall yield from D7]isopropyl amine was 7% and the isotopic

26 CFR 7.465-5 - Examples.

Science.gov (United States)

2010-04-01

... 26 Internal Revenue 14 2010-04-01 2010-04-01 false Examples. 7.465-5 Section 7.465-5 Internal... INCOME TAX REGULATIONS UNDER THE TAX REFORM ACT OF 1976 § 7.465-5 Examples. The provisions of § 7.465-1 and § 7.465-2 may be illustrated by the following examples: Example (1). J and K, as equal partners...

1 CFR 18.7 - Signature.

Science.gov (United States)

2010-01-01

... 1 General Provisions 1 2010-01-01 2010-01-01 false Signature. 18.7 Section 18.7 General Provisions... PREPARATION AND TRANSMITTAL OF DOCUMENTS GENERALLY § 18.7 Signature. The original and each duplicate original... stamped beneath the signature. Initialed or impressed signatures will not be accepted. Documents submitted...

4 CFR 25.7 - Gambling.

Science.gov (United States)

2010-01-01

... 4 Accounts 1 2010-01-01 2010-01-01 false Gambling. 25.7 Section 25.7 Accounts GOVERNMENT ACCOUNTABILITY OFFICE GENERAL PROCEDURES CONDUCT IN THE GOVERNMENT ACCOUNTABILITY OFFICE BUILDING AND ON ITS GROUNDS § 25.7 Gambling. Participating in games for money or other personal property or operating gambling...

33 CFR 332.7 - Management.

Science.gov (United States)

2010-07-01

... 33 Navigation and Navigable Waters 3 2010-07-01 2010-07-01 false Management. 332.7 Section 332.7... COMPENSATORY MITIGATION FOR LOSSES OF AQUATIC RESOURCES § 332.7 Management. (a) Site protection. (1) The... covenants. For government property, long-term protection may be provided through federal facility management...

32 CFR 1602.7 - Classification.

Science.gov (United States)

2010-07-01

... 32 National Defense 6 2010-07-01 2010-07-01 false Classification. 1602.7 Section 1602.7 National Defense Other Regulations Relating to National Defense SELECTIVE SERVICE SYSTEM DEFINITIONS § 1602.7 Classification. Classification is the exercise of the power to determine claims or questions with respect to...

42 CFR 9.7 - Reproduction.

Science.gov (United States)

2010-10-01

... 42 Public Health 1 2010-10-01 2010-10-01 false Reproduction. 9.7 Section 9.7 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL PROVISIONS STANDARDS OF CARE FOR CHIMPANZEES HELD IN THE FEDERALLY SUPPORTED SANCTUARY SYSTEM § 9.7 Reproduction. Chimpanzee reproduction is...

14 CFR 433.7 - Environmental.

Science.gov (United States)

2010-01-01

... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Environmental. 433.7 Section 433.7... TRANSPORTATION LICENSING LICENSE TO OPERATE A REENTRY SITE § 433.7 Environmental. An applicant shall provide the FAA with information for the FAA to analyze the environmental impacts associated with proposed...

Topical SMIP-7.7, a toll-like receptor 7 agonist, protects against genital herpes simplex virus type-2 disease in the guinea pig model of genital herpes.

Science.gov (United States)

Bernstein, David I; Cardin, Rhonda D; Bravo, Fernando J; Earwood, Julie; Clark, Jennifer R; Li, Yongkai; Mishra, Pranab; Li, Chun; Nayak, Bishnu P; Miller, Andrew T; Wu, Tom Y-H; Cooke, Michael P; Valiante, Nicholas M

2014-04-11

Development of more effective therapies for genital herpes simplex virus type-2 (HSV-2) infections remains a priority. The toll-like receptors (TLR) are attractive targets for the immunomodulation of primary and recurrent genital herpes infection. The guinea pig model of genital HSV-2 disease was therefore used to evaluate the efficacy of a new TLR-7 agonist, SMIP-7.7. The effects of SMIP-7.7 at concentrations between 0.90% and 0.09% were compared to the vehicle control or Aldara(®) (3M Health Care Limited, Northridge, CA, USA) as treatment for genital HSV-2 infections. Following intravaginal inoculation of Hartley guinea pigs with 10(6) pfu HSV-2 (MS strain), animals were treated intravaginally beginning at 36 h post-infection. Animals were evaluated for acute disease, acute virus replication, recurrent disease and shedding, as well as infection of the dorsal root ganglia. Treatment with SMIP-7.7 significantly decreased mean total lesion scores during primary infection (all doses, P<0.01 compared with vehicle control, and similar to Aldara(®)). Vaginal virus titres were reduced in treated animals compared with vehicle control (P<0.001 for each treatment versus vehicle control on day 4). Treatment with SMIP-7.7 also significantly decreased the number of recurrent lesion days, the number of days with recurrent virus shedding and the infection of the dorsal root ganglia compared to the vehicle control, and was similar to Aldara(®). As opposed to Aldara(®), SMIP-7.7 did not induce fever or weight loss during treatment. SMIP-7.7 improves the outcome of primary and recurrent HSV-2 disease comparable to Aldara(®) but without some of the side effects associated with Aldara(®).

36 CFR 331.7 - Sanitation.

Science.gov (United States)

2010-07-01

... 36 Parks, Forests, and Public Property 3 2010-07-01 2010-07-01 false Sanitation. 331.7 Section 331.7 Parks, Forests, and Public Property CORPS OF ENGINEERS, DEPARTMENT OF THE ARMY REGULATIONS..., KENTUCKY AND INDIANA § 331.7 Sanitation. (a) Garbage, trash, rubbish, litter, or any other waste material...

28 CFR 18.7 - Discovery.

Science.gov (United States)

2010-07-01

... 28 Judicial Administration 1 2010-07-01 2010-07-01 false Discovery. 18.7 Section 18.7 Judicial Administration DEPARTMENT OF JUSTICE OFFICE OF JUSTICE PROGRAMS HEARING AND APPEAL PROCEDURES § 18.7 Discovery.... Such order may be entered upon a showing that the deposition is necessary for discovery purposes, and...

6 CFR 7.2 - Scope.

Science.gov (United States)

2010-01-01

..., National Industrial Security Program, and its implementing directives. (c) This part is independent of and... 6 Domestic Security 1 2010-01-01 2010-01-01 false Scope. 7.2 Section 7.2 Domestic Security DEPARTMENT OF HOMELAND SECURITY, OFFICE OF THE SECRETARY CLASSIFIED NATIONAL SECURITY INFORMATION § 7.2 Scope...

45 CFR 707.7 - Employment.

Science.gov (United States)

2010-10-01

... 45 Public Welfare 3 2010-10-01 2010-10-01 false Employment. 707.7 Section 707.7 Public Welfare... § 707.7 Employment. No qualified individual with disabilities shall, on the basis of disability, be subjected to discrimination in employment under any program or activity conducted by the Agency. The...

23 CFR 657.7 - Objective.

Science.gov (United States)

2010-04-01

... 23 Highways 1 2010-04-01 2010-04-01 false Objective. 657.7 Section 657.7 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION ENGINEERING AND TRAFFIC OPERATIONS CERTIFICATION OF SIZE AND WEIGHT ENFORCEMENT § 657.7 Objective. The objective of this regulation is the development and operation by each State...

Slc39a7/zip7 plays a critical role in development and zinc homeostasis in zebrafish.

Directory of Open Access Journals (Sweden)

Guang Yan

Full Text Available BACKGROUND: Slc39a7/Zip7, also known as Ke4, is a member of solute carrier family 39 (Slc39a and plays a critical role in regulating cell growth and death. Because the function of Zip7 in vivo was unclear, the present study investigated the function of zip7 in vertebrate development and zinc metabolism using zebrafish as a model organism. PRINCIPAL FINDING: Using real-time PCR to determine the gene expression pattern of zip7 during zebrafish development, we found that zip7 mRNA is expressed throughout embryonic development and into maturity. Interestingly, whole mount in situ hybridization revealed that while zip7 mRNA is ubiquitously expressed until 12 hours post-fertilization (hpf; at 24 hpf and beyond, zip7 mRNA was specifically detected only in eyes. Morpholino-antisense (MO gene knockdown assay revealed that downregulation of zip7 expression resulted in several morphological defects in zebrafish including decreased head size, smaller eyes, shorter palates, and shorter and curved spinal cords. Analysis by synchrotron radiation X-ray fluorescence (SR-XRF showed reduced concentrations of zinc in brain, eyes, and gills of zip7-MO-injected embryos. Furthermore, incubation of the zip7 knockdown embryos in a zinc-supplemented solution was able to rescue the MO-induced morphological defects. SIGNIFICANCE: Our data suggest that zip7 is required for eye, brain, and skeleton formation during early embryonic development in zebrafish. Moreover, zinc supplementation can partially rescue defects resulting from zip7 gene knockdown. Taken together, our data provide critical insight into a novel function of zip7 in development and zinc homeostasis in vivo in zebrafish.

Targeting B7x and B7-H3 as New Immunotherapies for Prostate Cancer

Science.gov (United States)

2016-09-01

activated and express receptors for B7x and B7-H3 and human prostate cancer cells express B7x or B7-H3. FACS showed the approach how we identified human...Immunomodu- latory pathways include members of the TNF receptor family and their ligands which have been studied as targets for cancer immunotherapy. These...urothelial bladder cancer patients resulting in an FDA breakthrough designation [50], and MSB0010718C which exhibits antitumor activ- ity by blocking PD-L1

Thermal rearrangement of 7-methylbicyclo

Science.gov (United States)

Bender; Leber; Lirio; Smith

2000-08-25

The gas-phase thermal rearrangement of exo-7-methylbicyclo[3.2.0]hept-2-ene yields almost exclusively 5-methylnorbornene products. Inversion (i) of configuration dominates this [1,3] sigmatropic shift although some retention (r) is also observed. Because the [1,3] migration can only occur suprafacially (s) in this geometrically constrained system, the si/sr ratio of 7 observed for the migration of C7 in exo-7-methylbicyclo[3.2.0]hept-2-ene indicates that the orbital symmetry rules are somewhat permissive for the [1,3] sigmatropic migration of carbon.

49 CFR 220.7 - Penalty.

Science.gov (United States)

2010-10-01

..., or has caused death or injury, a penalty not to exceed $100,000 per violation may be assessed; and... 49 Transportation 4 2010-10-01 2010-10-01 false Penalty. 220.7 Section 220.7 Transportation Other... TRANSPORTATION RAILROAD COMMUNICATIONS General § 220.7 Penalty. Any person (including but not limited to a...

43 CFR 24.7 - Exemptions.

Science.gov (United States)

2010-10-01

... 43 Public Lands: Interior 1 2010-10-01 2010-10-01 false Exemptions. 24.7 Section 24.7 Public Lands...-FEDERAL RELATIONSHIPS § 24.7 Exemptions. (a) Exempted from this policy are the following: (1) The control and regulation by the United States, in the area in which an international convention or treaty...

9 CFR 311.7 - Arthritis.

Science.gov (United States)

2010-01-01

... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Arthritis. 311.7 Section 311.7 Animals... CERTIFICATION DISPOSAL OF DISEASED OR OTHERWISE ADULTERATED CARCASSES AND PARTS § 311.7 Arthritis. (a) Carcasses affected with arthritis which is localized and not associated with systemic change may be passed for human...

29 CFR 101.7 - Settlements.

Science.gov (United States)

2010-07-01

... 29 Labor 2 2010-07-01 2010-07-01 false Settlements. 101.7 Section 101.7 Labor Regulations Relating... Section 10 (a) to (i) of the Act and Telegraph Merger Act Cases § 101.7 Settlements. Before any complaint... for the submission and consideration of facts, argument, offers of settlement, or proposals of...

6 CFR 13.7 - Complaint.

Science.gov (United States)

2010-01-01

... 6 Domestic Security 1 2010-01-01 2010-01-01 false Complaint. 13.7 Section 13.7 Domestic Security DEPARTMENT OF HOMELAND SECURITY, OFFICE OF THE SECRETARY PROGRAM FRAUD CIVIL REMEDIES § 13.7 Complaint. (a) On or after the date the Department of Justice approves the issuance of a Complaint in accordance...

12 CFR 7.5000 - Scope.

Science.gov (United States)

2010-01-01

... 12 Banks and Banking 1 2010-01-01 2010-01-01 false Scope. 7.5000 Section 7.5000 Banks and Banking COMPTROLLER OF THE CURRENCY, DEPARTMENT OF THE TREASURY BANK ACTIVITIES AND OPERATIONS Electronic Activities § 7.5000 Scope. This subpart applies to a national bank's use of technology to deliver services and...

42 CFR 1005.7 - Discovery.

Science.gov (United States)

2010-10-01

... 42 Public Health 5 2010-10-01 2010-10-01 false Discovery. 1005.7 Section 1005.7 Public Health... OF EXCLUSIONS, CIVIL MONEY PENALTIES AND ASSESSMENTS § 1005.7 Discovery. (a) A party may make a... and any forms of discovery, other than those permitted under paragraph (a) of this section, are not...

29 CFR 102.7 - State.

Science.gov (United States)

2010-07-01

... 29 Labor 2 2010-07-01 2010-07-01 false State. 102.7 Section 102.7 Labor Regulations Relating to Labor NATIONAL LABOR RELATIONS BOARD RULES AND REGULATIONS, SERIES 8 Definitions § 102.7 State. The term State as used herein shall include the District of Columbia and all States, Territories, and possessions...

7 CFR 201.56-7 - Lily family, Liliaceae.

Science.gov (United States)

2010-01-01

..., Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) FEDERAL SEED ACT FEDERAL SEED ACT REGULATIONS Germination Tests in the Administration of the Act § 201.56-7 Lily family, Liliaceae. Kinds of seed: Asparagus, chives, leek, onion, and Welsh onion. (a) Asparagus. (1) General description. (i...

25 CFR 122.7 - Budget.

Science.gov (United States)

2010-04-01

... 25 Indians 1 2010-04-01 2010-04-01 false Budget. 122.7 Section 122.7 Indians BUREAU OF INDIAN... § 122.7 Budget. (a) By August 1 of each year, the Osage Tribal Education Committee will submit a proposed budget to the Assistant Secretary or to his/her designated representative for formal approval...

22 CFR 8.7 - Security.

Science.gov (United States)

2010-04-01

... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Security. 8.7 Section 8.7 Foreign Relations DEPARTMENT OF STATE GENERAL ADVISORY COMMITTEE MANAGEMENT § 8.7 Security. (a) All officers and members of a committee must have a security clearance for the subject matter level of security at which the committee...

36 CFR 520.7 - Gambling.

Science.gov (United States)

2010-07-01

... 36 Parks, Forests, and Public Property 3 2010-07-01 2010-07-01 false Gambling. 520.7 Section 520.7... BUILDINGS AND GROUNDS OF THE NATIONAL ZOOLOGICAL PARK OF THE SMITHSONIAN INSTITUTION § 520.7 Gambling. Participating in games for money or other personal property or the operation of gambling devices, the conduct of...

31 CFR 7.8 - Delegation.

Science.gov (United States)

2010-07-01

... 31 Money and Finance: Treasury 1 2010-07-01 2010-07-01 false Delegation. 7.8 Section 7.8 Money and Finance: Treasury Office of the Secretary of the Treasury EMPLOYEE INVENTIONS § 7.8 Delegation. The heads of bureaus or offices and the General Counsel may delegate, as appropriate, the performance of the...

43 CFR 7.19 - Report.

Science.gov (United States)

2010-10-01

... 43 Public Lands: Interior 1 2010-10-01 2010-10-01 false Report. 7.19 Section 7.19 Public Lands... § 7.19 Report. (a) Each Federal land manager, when requested by the Secretary of the Interior, will... comprehensively report on activities carried out under provisions of the Act. (b) The Secretary of the Interior...

17 CFR 9.7 - Settlement.

Science.gov (United States)

2010-04-01

... 17 Commodity and Securities Exchanges 1 2010-04-01 2010-04-01 false Settlement. 9.7 Section 9.7... DISCIPLINARY, ACCESS DENIAL OR OTHER ADVERSE ACTIONS General Provisions § 9.7 Settlement. At any time before... accordance with § 9.20, the parties may file a stipulation for dismissal based on a settlement agreement...

14 CFR 381.7 - Advertising.

Science.gov (United States)

2010-01-01

... REGULATIONS SPECIAL EVENT TOURS § 381.7 Advertising. No operator of a Special Event Tour or agent of such an operator shall conduct, or cause or allow to be conducted, any advertising, solicitation or other promotion... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Advertising. 381.7 Section 381.7...

42 CFR 4.7 - Fees.

Science.gov (United States)

2010-10-01

... 42 Public Health 1 2010-10-01 2010-10-01 false Fees. 4.7 Section 4.7 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL PROVISIONS NATIONAL LIBRARY OF MEDICINE § 4.7 Fees. The Director may charge reasonable fees for any service provided by the Library under this part...

32 CFR 1285.7 - Reports.

Science.gov (United States)

2010-07-01

... 32 National Defense 6 2010-07-01 2010-07-01 false Reports. 1285.7 Section 1285.7 National Defense... AGENCY FREEDOM OF INFORMATION ACT PROGRAM § 1285.7 Reports. The reporting requirement outlined in this rule is assigned Report Control Symbol DD-PA(A)1365 and will be prepared according to part 286, subpart...

34 CFR 303.7 - Children.

Science.gov (United States)

2010-07-01

... 34 Education 2 2010-07-01 2010-07-01 false Children. 303.7 Section 303.7 Education Regulations of... General Purpose, Eligibility, and Other General Provisions § 303.7 Children. As used in this part, children means infants and toddlers with disabilities as that term is defined in § 303.16. (Authority: 20 U...

45 CFR 501.7 - Time.

Science.gov (United States)

2010-10-01

... 45 Public Welfare 3 2010-10-01 2010-10-01 false Time. 501.7 Section 501.7 Public Welfare..., DEPARTMENT OF JUSTICE RULES OF PRACTICE SUBPOENAS, DEPOSITIONS, AND OATHS § 501.7 Time. (a) Computation. In computing any period of time prescribed or allowed by the regulations, by order of the Commission, or by any...

33 CFR 8.7 - Information.

Science.gov (United States)

2010-07-01

... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Information. 8.7 Section 8.7... GUARD RESERVE § 8.7 Information. (a) Information concerning the Coast Guard Reserve may be obtained from Commandant (CG-13), 2100 2nd St. SW., Stop 7801, Washington, DC 20593-7801. (b) Information and requirements...

44 CFR 7.942 - Mediation.

Science.gov (United States)

2010-10-01

... 44 Emergency Management and Assistance 1 2010-10-01 2010-10-01 false Mediation. 7.942 Section 7..., Conciliation, and Enforcement Procedures § 7.942 Mediation. (a) FEMA will promptly refer to a mediation agency... participate in the mediation process to the extent necessary to reach an agreement or for the mediator to make...

Inhibition of translation by 7-methyl guanosine (m7G) nucleotide cap analogs with derivatized 5'-monophosphates

International Nuclear Information System (INIS)

Tahara, S.M.; Darzynkiewicz, E.; Ekiel, I.

1986-01-01

Recognition of the 5'-m 7 GpppN (cap) structure of eukaryote mRNA is an important step of translation initiation as shown by the potent inhibitory effect of m 7 G nucleotides on this process. A comparison of cap analogs as competitive inhibitors of initiation has allowed the authors to map probable protein-ligand contact points between the cap and cognate cap binding proteins (CBPs). Recently, several new derivatives of m 7 GMP (1) with modified phosphates were synthesized: m 7 G 5'-phosphite (2), m 7 G 5'-phosphoramidate (3), m 7 G 5'-methylphosphonate (4), and m 7 G 5'-phosphate-O-methyl ester (5). In addition, 7,8-dimethyl GMP (6) and 7-methyl 8-amino GMP (7) were synthesized. 6 and7 are primarily syn and anti respectively, relative to the glycosidic bond as shown by solution NMR studies. Inhibition by analogs on total translation in reticulocyte lysate and binding of mRNA to rabbit reticulocyte ribosomes was found to be: 1 = 3 > 5 > 4 > 2. The inhibitory activity of 3 was unexpected since it is isosteric with 4, however it suggested that electron configuration and/or the ability to form a hydrogen bond between protein and the phosphate moiety might be important for ligand binding. 7 was more inhibitory than 6. The latter two are isosteric therefore differences in electron delocalization and/or syn-anti conformation are likely to be the reason(s) for the observed difference

Structural Basis for Inhibition of Mycobacterial and Human Adenosine Kinase by 7-Substituted 7-(Het)aryl-7-deazaadenine Ribonucleosides

Czech Academy of Sciences Publication Activity Database

Snášel, Jan; Nauš, Petr; Dostál, Jiří; Hnízda, Aleš; Fanfrlík, Jindřich; Brynda, Jiří; Bourderioux, Aurelie; Dušek, Michal; Dvořáková, H.; Stolaříková, J.; Zábranská, Helena; Pohl, Radek; Konečný, P.; Džubák, P.; Votruba, Ivan; Hajdúch, M.; Řezáčová, Pavlína; Veverka, Václav; Hocek, Michal; Pichová, Iva

2014-01-01

Roč. 57, č. 20 (2014), s. 8268-8279 ISSN 0022-2623 R&D Projects: GA ČR GAP207/11/0344; GA MŠk LO1302; GA MŠk(CZ) LK11205 EU Projects: European Commission(XE) 241587 - SYSTEMTB Grant - others:GA MŠk(CZ) LM2011020 Institutional support: RVO:61388963 ; RVO:68378271 Keywords : 7-(het)aryl-7-deazaadenine ribonucleosides * enzyme inhibition * adenosine kinase * cytostatic activity Subject RIV: CC - Organic Chemistry Impact factor: 5.447, year: 2014

31 CFR 91.7 - Gambling.

Science.gov (United States)

2010-07-01

... 31 Money and Finance: Treasury 1 2010-07-01 2010-07-01 false Gambling. 91.7 Section 91.7 Money and... BUREAU OF THE MINT BUILDINGS AND GROUNDS § 91.7 Gambling. (a) Participating in games for money or other property, the operation of gambling devices, the conduct of a lottery or pool, the selling or purchasing of...

39 CFR 965.7 - Default.

Science.gov (United States)

2010-07-01

... 39 Postal Service 1 2010-07-01 2010-07-01 false Default. 965.7 Section 965.7 Postal Service UNITED STATES POSTAL SERVICE PROCEDURES RULES OF PRACTICE IN PROCEEDINGS RELATIVE TO MAIL DISPUTES § 965.7 Default. A party who fails to file the submittal required by § 965.5 may be held in default and the...

15 CFR 766.7 - Default.

Science.gov (United States)

2010-01-01

... 15 Commerce and Foreign Trade 2 2010-01-01 2010-01-01 false Default. 766.7 Section 766.7 Commerce... PROCEEDINGS § 766.7 Default. (a) General. Failure of the respondent to file an answer within the time provided... aside default—(1) Procedure. Upon petition filed by a respondent against whom a default order has been...

12 CFR 213.7 - Advertising.

Science.gov (United States)

2010-01-01

... 12 Banks and Banking 2 2010-01-01 2010-01-01 false Advertising. 213.7 Section 213.7 Banks and... (REGULATION M) § 213.7 Advertising. (a) General rule. An advertisement for a consumer lease may state that a... paragraph (d)(1) of this section shall also state the following items: (i) That the transaction advertised...

38 CFR 7.6 - Benefits.

Science.gov (United States)

2010-07-01

... 38 Pensions, Bonuses, and Veterans' Relief 1 2010-07-01 2010-07-01 false Benefits. 7.6 Section 7.6... Soldiers' and Sailors' Civil Relief Act Amendments of 1942 § 7.6 Benefits. Any policy found to be entitled... such monetary benefits arising out of the contract or by reason thereof, will be held subject to...

12 CFR 583.7 - Control.

Science.gov (United States)

2010-01-01

... 12 Banks and Banking 5 2010-01-01 2010-01-01 false Control. 583.7 Section 583.7 Banks and Banking... AND LOAN HOLDING COMPANIES § 583.7 Control. For purposes of this chapter, a person shall be deemed to have control of: (a) A savings association if the person directly or indirectly or acting in concert...

22 CFR 130.7 - Supplier.

Science.gov (United States)

2010-04-01

... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Supplier. 130.7 Section 130.7 Foreign Relations... § 130.7 Supplier. Supplier means any person who enters into a contract with the Department of Defense for the sale of defense articles or defense services valued in an amount of $500,000 or more under...

Windows 7 The Missing Manual

CERN Document Server

Pogue, David

2010-01-01

In early reviews, geeks raved about Windows 7. But if you're an ordinary mortal, learning what this new system is all about will be challenging. Fear not: David Pogue's Windows 7: The Missing Manual comes to the rescue. Like its predecessors, this book illuminates its subject with reader-friendly insight, plenty of wit, and hardnosed objectivity for beginners as well as veteran PC users. Windows 7 fixes many of Vista's most painful shortcomings. It's speedier, has fewer intrusive and nagging screens, and is more compatible with peripherals. Plus, Windows 7 introduces a slew of new features,

As notícias sem jornalistas: uma ameaça real ou uma história de terror?

Directory of Open Access Journals (Sweden)

Erik Neveu

2010-06-01

Full Text Available O jornalismo como profissão e know-how está preso em um turbilhão de mudanças O fim dos jornais não significaria automaticamente o fim do jornalismo ou dos jornalistas, mas fica difícil imaginar como o colapso da instituição, poderia ocorrer sem provocar um terremoto na definição e na prática do jornalismo, que, como cultura profissional com habilidades codificadas e características, corre o risco de ser diluído e transformado no contínuo nebuloso daqueles já chamados “funcionários da informação”. Este artigo vai sugerir como várias tendências contemporâneas estão desafiando e redefinindo a prática jornalística. O objetivo aqui é defender a possibilidade de definir o jornalista como alguém que coleciona fatos que não estão numa tela no seu escritório, como alguém que fala para as audiências que não sejam apenas consumidores e como alguém que mantém autonomia suficiente para praticar as aptidõesde um verificador crítico das notícias.

Caracterização de novos isolados de Bacillus thuringiensis para o controle de importantes insetos-praga da agricultura

Directory of Open Access Journals (Sweden)

Emeline Boni Campanini

2012-01-01

Full Text Available A bactéria Bacillus thuringiensis Berliner produz um corpo de inclusão paraesporal (cristal de natureza proteica, formado durante a esporulação, que atua de forma eficiente no controle de insetos-praga de culturas economicamente importantes. Esse cristal é constituído de proteínas Cry, que são codificadas pelos genes cry; um isolado pode ser caracterizado pelo conteúdo de genes cry que apresenta. Visando caracterizar novos isolados no combate de insetos-praga pertencentes às ordens Lepidoptera e Coleoptera, 76 isolados bacterianos foram analisados molecularmente e tiveram seu potencial de controle avaliado por meio de bioensaios com larvas de Spodoptera frugiperda (J.E. Smith, Sphenophorus levis Vaurie e Tenebrio molitor Linnaeus. As análises moleculares indicaram 11 isolados (14,5% da coleção, contendo genes lepidóptero-específicos e 17 (22,37% com genes coleóptero-específicos. As análises de patogenicidade revelaram dois isolados com alto potencial de controle para lagartas de S. frugiperda, um para larvas de S. levis e seis prejudiciais ao desenvolvimento das larvas de T. molitor. Esses isolados de B. thuringiensis podem ser promissores no controle biológico das referidas pragas.

Ocorrência de fungos filamentosos termo-resistentes em polpa de tomate envasada assepticamente

Directory of Open Access Journals (Sweden)

BAGLIONI Flávio

1999-01-01

Full Text Available Este trabalho teve como objetivo principal determinar a ocorrência de fungos filamentosos termo-resistentes durante o processamento asséptico de polpa de tomate (8° BRIX. Durante o período de safra de tomate foram feitas amostragens em 9 lotes (3 no início, 3 no pico e 3 em fim de safra e no período de entresafra em 5 lotes. Foi feita a enumeração de fungos termo-resistentes nas amostras coletadas durante as diferentes etapas do processo asséptico de cada lote. Foram obtidas contagens médias relativamente baixas, variando entre <1 e 8UFC/100mL de amostra. As maiores contagens foram obtidas na matéria prima e na água de pré lavagem e transporte. Cinqüenta linhagens de fungos termo-resistentes detectadas no procedimento de enumeração foram isoladas, codificadas e estocadas. Os esporos de cada isolado com 1 mês de cultivo foram submetidos a diferentes choques térmicos para selecionar a linhagem de fungo mais termo-resistente. O isolado de fungo mais termo-resistente (sobrevivência ao choque à 100° C/25 minutos foi identificado como Neosartorya fischeri.

El algoritmo HyRPNI y una aplicación en bioinformática

Directory of Open Access Journals (Sweden)

Gloria Inés Alvarez V.

2011-06-01

Full Text Available Proponemos un algoritmo de inferencia gramatical para lenguajes regulares que permite ahorrar cómputo al usar dos criterios diferentes para elegir los estados a ser procesados, un criterio se usa en la primera fase del proceso de inferencia (al principio y el otro en el resto del proceso. Realizamos experimentos para observar el desempeño del algoritmo, para aprender sobre el tamaño ideal de su primera fase y para mostrar su aplicación en la solución de un problema específico en bioinformática: la predicción de sitios de corte en poliproteínas codificadas por virus de la familia Potyviridae./ We propose a grammar inference algorithm for regular languages which saves computational cost by using two different criteria to choose states to be processed: one in the first phase of the inference process (the beginning and another for the rest of the process. We applied experiments to observe performance of the algorithm, to learn about the best size of its first phase and to show results of its application to solve a specific problem in Bioinformatics: the cleavage site prediction problem in polyproteins encoded by viruses of the Potyviridae family.

Uma tecnologia com múltiplas aplicações

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Pinheiro Márcia Margis

2000-01-01

Full Text Available O melhoramento vegetal vem sendo praticado pelo homem há milhares de anos. Entretanto, esse processo de domesticação tornou os vegetais mais susceptíveis a pragas e doenças. O melhoramento clássico permitiu, por cruzamento, a manipulação genética dos vegetais com conseqüente aumento na produtividade agrícola. Recentemente, a tecnologia do DNA recombinante ampliou as possibilidades de integração de genes exógenos ao genoma vegetal, resultando na produção das plantas transgênicas. Apesar das grandes discussões em torno do assunto, essas plantas representam hoje um caminho promissor para o melhoramento vegetal. Inúmeros exemplos de estratégias de transferência de genes conferiram, com sucesso, resistência a herbicidas, vírus, fungos, bactérias e insetos ou produziram um aumento na qualidade dos alimentos. Além das aplicações biotecnológicas, as plantas transgênicas têm contribuído significativamente para o estudo do funcionamento dos genes, tais como a análise da regulação da expressão gênica e o estudo das funções das proteínas codificadas pelos diferentes genes da planta.

Resistencia a los antibióticos beta-lactámicos Carbapenems mediada por el gen blaKPC en Klebsiella pneumoniae

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Aura Dayana del Carmen Falco Restrepo

2015-12-01

Full Text Available  Los carbapenems son antibióticos â-lactamicos de amplio espectro que se utilizan como terapia de primera línea para tratar pacientes con infecciones graves. Actualmente, la resistencia a carbapenems se asocia con la presencia de â-lactamasas capaces de hidrolizar estos antibióticos. La carbapenemasa de clase A más común es KPC, la cual es codificada por el gen blaKPC que generalmente, se encuentra ubicado en plásmidos. La selección y la rápida propagación de aislados de Klebsiella pneumoniae portadores de estas carbapenemasas se ha convertido en un problema de salud pública a nivel mundial. Esta mini-revision describe uno de los mecanismos más frecuentes de resistencia a carbapenems como lo son las enzimas carbapenemasas tipo KPC. Además se describen las variantes del gen blaKPC asi como el elemento genético móvil, el transposon Tn4401, el principal responsable de su diseminación entre géneros y especies bacterianas diferentes. Finalmente se hace una pequeña revisión cronológica de los reportes de la enzima KPC a nivel mundial.

A randomized clinical trial of an inactivated avian influenza A (H7N7 vaccine.

Directory of Open Access Journals (Sweden)

Robert B Couch

Full Text Available BACKGROUND: Concern for a pandemic caused by a newly emerged avian influenza A virus has led to clinical trials with candidate vaccines as preparation for such an event. Most trials have involved vaccines for influenza A (H5N1, A (H7N7 or A (H9N2. OBJECTIVE: To evaluate dosage-related safety and immunogenicity of an inactivated influenza A (H7N7 vaccine in humans. DESIGN: One hundred twenty-five healthy young adults were randomized to receive two doses intramuscularly of placebo or 7.5, 15, 45 or 90 µg of HA of an inactivated subunit influenza A (H7N7 vaccine (25 per group, four weeks apart. Reactogenicity was evaluated closely for one week and for any adverse effect for six months after each dose. Serum hemagglutination-inhibiting and neutralizing antibody responses were determined four weeks after each dose and at six months. RESULTS: Reactogenicity evaluations indicated the vaccinations were well tolerated. Only one subject developed a ≥4-fold serum hemagglutination-inhibition (HAI antibody response and a final titer of ≥1:40 four weeks after dose two and only five subjects developed a neutralizing antibody rise and a final titer of ≥1:40 in tests performed at a central laboratory. Four of the five were given the 45 or 90 µg HA dosage. A more sensitive HAI assay at the study site revealed a dose-response with increasing HA dosage but only 36% in the 90 µg HA group developed a ≥4-fold rise in antibody in this test and only one of these achieved a titer of ≥1:32. CONCLUSION: This inactivated subunit influenza A (H7N7 vaccine was safe but poorly immunogenic in humans. TRIALS REGISTRATION: ClinicalTrials.gov NCT00546585.

Begining Java EE 7

CERN Document Server

Gonclaves, Antonio

2013-01-01

Java Enterprise Edition (Java EE) continues to be one of the leading Java technologies and platforms. Beginning Java EE 7 is the first tutorial book on Java EE 7. Step by step and easy to follow, this book describes many of the Java EE 7 specifications and reference implementations, and shows them in action using practical examples. This definitive book also uses the newest version of GlassFish to deploy and administer the code examples. Written by an expert member of the Java EE specification request and review board in the Java Community Process (JCP), this book contains the best information possible, from an expert’s perspective on enterprise Java technologies.

25 CFR 212.7 - Environmental studies.

Science.gov (United States)

2010-04-01

... 25 Indians 1 2010-04-01 2010-04-01 false Environmental studies. 212.7 Section 212.7 Indians BUREAU OF INDIAN AFFAIRS, DEPARTMENT OF THE INTERIOR ENERGY AND MINERALS LEASING OF ALLOTTED LANDS FOR MINERAL DEVELOPMENT General § 212.7 Environmental studies. The provisions of § 211.7 of this subchapter...

49 CFR 7.4 - Publication required.

Science.gov (United States)

2010-10-01

... 49 Transportation 1 2010-10-01 2010-10-01 false Publication required. 7.4 Section 7.4 Transportation Office of the Secretary of Transportation PUBLIC AVAILABILITY OF INFORMATION Information Required To Be Made Public by DOT § 7.4 Publication required. (a) General. The material described in § 7.3...

49 CFR 216.7 - Penalties.

Science.gov (United States)

2010-10-01

... hazard of death or injury to persons, or has caused death or injury, a penalty not to exceed $100,000 per... 49 Transportation 4 2010-10-01 2010-10-01 false Penalties. 216.7 Section 216.7 Transportation... § 216.7 Penalties. Any person (an entity of any type covered under 1 U.S.C. 1, including but not limited...

Fox-7 for Insensitive Boosters

Science.gov (United States)

2010-08-01

cavitation , and therefore nucleation, to occur at each frequency. As well as producing ultrasound at different frequencies, the method of delivery of...processing techniques using ultrasound , designed to optimise FOX-7 crystal size and morphology to improve booster formulations, and results from these...7 booster formulations. Also included are particle processing techniques using ultrasound , designed to optimise FOX-7 crystal size and morphology

7 CFR 33.7 - Less than carload lot.

Science.gov (United States)

2010-01-01

... Regulations of the Department of Agriculture AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing... ISSUED UNDER AUTHORITY OF THE EXPORT APPLE ACT Definitions § 33.7 Less than carload lot. Less than carload lot means a quantity of apples in packages not exceeding 20,000 pounds gross weight or 400...

Gene variation in IL-7 receptor (IL-7R)α affects IL-7R response in CD4+ T cells in HIV-infected individuals

DEFF Research Database (Denmark)

Hartling, Hans Jakob; Ryder, Lars P.; Ullum, Henrik

2017-01-01

Optimal CD4+ T cell recovery after initiating combination antiretroviral treatment (cART) in HIV infection reduces risk of morbidity and mortality. T-allele homozygosity (‘TT’) in the single nucleotide polymorphism, rs6897932(C/T), in the IL-7 receptor α (IL-7RA) is associated with faster CD4+ T...... cell recovery after cART initiation compared to C-allele homozygosity in rs6897932 (‘CC’). However, underlying mechanisms are unknown. We aimed to examine potential mechanisms explaining the association between rs6897932 and CD4+ T cell recovery. Ten ‘TT’ and 10 ‘CC’ HIV-infected individuals matched...... on gender, age, and nadir and current CD4+ T cell counts were included in a cross-sectional study. ‘TT’ individuals had higher proportion of CD4+ T cells expressing pSTAT5 compared to ‘CC’ individuals after stimulating with IL-7, especially when co-stimulated with soluble IL7-RA (sIL-7RA). Furthermore, ‘TT...

Interferon β induces clearance of mutant ataxin 7 and improves locomotion in SCA7 knock-in mice.

Science.gov (United States)

Chort, Alice; Alves, Sandro; Marinello, Martina; Dufresnois, Béatrice; Dornbierer, Jean-Gabriel; Tesson, Christelle; Latouche, Morwena; Baker, Darren P; Barkats, Martine; El Hachimi, Khalid H; Ruberg, Merle; Janer, Alexandre; Stevanin, Giovanni; Brice, Alexis; Sittler, Annie

2013-06-01

We showed previously, in a cell model of spinocerebellar ataxia 7, that interferon beta induces the expression of PML protein and the formation of PML protein nuclear bodies that degrade mutant ataxin 7, suggesting that the cytokine, used to treat multiple sclerosis, might have therapeutic value in spinocerebellar ataxia 7. We now show that interferon beta also induces PML-dependent clearance of ataxin 7 in a preclinical model, SCA7(266Q/5Q) knock-in mice, and improves motor function. Interestingly, the presence of mutant ataxin 7 in the mice induces itself the expression of endogenous interferon beta and its receptor. Immunohistological studies in brains from two patients with spinocerebellar ataxia 7 confirmed that these modifications are also caused by the disease in humans. Interferon beta, administered intraperitoneally three times a week in the knock-in mice, was internalized with its receptor in Purkinje and other cells and translocated to the nucleus. The treatment induced PML protein expression and the formation of PML protein nuclear bodies and decreased mutant ataxin 7 in neuronal intranuclear inclusions, the hallmark of the disease. No reactive gliosis or other signs of toxicity were observed in the brain or internal organs. The performance of the SCA7(266Q/5Q) knock-in mice was significantly improved on two behavioural tests sensitive to cerebellar function: the Locotronic® Test of locomotor function and the Beam Walking Test of balance, motor coordination and fine movements, which are affected in patients with spinocerebellar ataxia 7. In addition to motor dysfunction, SCA7(266Q/5Q) mice present abnormalities in the retina as in patients: ataxin 7-positive neuronal intranuclear inclusions that were reduced by interferon beta treatment. Finally, since neuronal death does not occur in the cerebellum of SCA7(266Q/5Q) mice, we showed in primary cell cultures expressing mutant ataxin 7 that interferon beta treatment improves Purkinje cell survival.

Indirubin Derivative 7-Bromoindirubin-3-Oxime (7Bio Attenuates Aβ Oligomer-Induced Cognitive Impairments in Mice

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Liping Chen

2017-11-01

Full Text Available Indirubins are natural occurring alkaloids extracted from indigo dye-containing plants. Indirubins could inhibit various kinases, and might be used to treat chronic myelocytic leukemia, cancer and neurodegenerative disorders. 7-bromoindirubin-3-oxime (7Bio, an indirubin derivative derived from indirubin-3-oxime, possesses inhibitory effects against cyclin-dependent kinase-5 (CDK5 and glycogen synthase kinase-3β (GSK3β, two pharmacological targets of Alzheimer's disease (AD. In this study, we have discovered that 2.3–23.3 μg/kg 7Bio effectively prevented β-amyloid (Aβ oligomer-induced impairments of spatial cognition and recognition without affecting bodyweight and motor functions in mice. Moreover, 7Bio potently inhibited Aβ oligomer-induced expression of interleukin-6 (IL-6 and tumor necrosis factor-α (TNF-α. Furthermore, 7Bio significantly prevented the decreased expression of synapsin-1 and PSD-95, biomarkers of pre-synaptic and post-synaptic proteins in Aβ oligomer-treated mice. The mean optical density (OD with hyper-phosphorylated tau (pTau, glial fibrillary acidic protein (GFAP and CD45 positive staining in the hippocampus of 7Bio-treated mice were significantly decreased compared to those of Aβ oligomer-treated mice. In addition, Western blotting analysis showed that 7Bio attenuated Aβ oligomer-decreased expression of pSer9-GSK3β. Those results suggested that 7Bio could potently inhibit Aβ oligomer-induced neuroinflammation, synaptic impairments, tau hyper-phosphorylation, and activation of astrocytes and microglia, which may contribute to the neuroprotective effects of 7Bio. Based on these findings, we expected that 7Bio might be developed as a novel anti-AD lead compound.

Circulating IGF-binding protein 7 (IGFBP7 levels are elevated in patients with endometriosis or undergoing diabetic hemodialysis

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Sasajima Koji

2008-11-01

Full Text Available Abstract Background Insulin-like growth factor-binding protein-7 (IGFBP7 is a secretory protein with a molecular mass of approximately 30 kDa. It is abundantly expressed in the uterine endometrium during the secretory phase of the menstrual cycle. Decreased IGFBP7 expression has been observed in some cancers and leiomyomata. Methods To determine whether serum IGFBP7 levels reflect changes in uterine IGFBP7 expression in humans during the menstrual cycle, and to examine whether serum IGFBP7 levels are altered in patients with various disorders, we developed a novel, dual-antibody sandwich enzyme-linked immunosorbent assay (ELISA. Firstly, concentrations of IGFBP7 released into the medium were determined in cultured endometrial stromal and glandular cells. Blood samples were collected from women who had normal menstrual cycles and who had been diagnosed with endometriosis. Serum from hemodialysis patients and gastrointestinal cancers was also used to determine the IGFBP7 levels. Results Using this new ELISA, we demonstrated that cultured uterine cells secrete IGFBP7 into the medium. Patients with endometriosis and those with type II diabetes mellitus undergoing hemodialysis had significantly higher serum concentrations of IGFBP7 than the relevant control subjects. There were no differences in serum IGFBP7 levels in women at different stages of the menstrual cycle. Furthermore, serum IGFBP7 levels in patients with colorectal, esophageal, or endometrial cancer were not different than normal healthy subjects. Conclusion Our observations suggest that IGFBP7 is associated with the pathophysiology of endometriosis and diabetes mellitus, and that serum IGFBP7 levels do not reflect enhanced uterine expression of IGFBP7 mRNA during the menstrual cycle.

Java EE 7 first look

CERN Document Server

Fabrice, Armel

2013-01-01

An easy-to-follow guide to reveal the new features of Java EE 7 and how to efficiently utilize them.Given the main objectives pursued, this book targets three groups of people with a knowledge of the Java language. They are:Beginners in the Java EE platform who would like to have an idea about the main specifications of Java EE 7.Developers who have experimented with previous versions of Java EE and who would like to explore the new features of Java EE 7.Building architects who want to learn how to put together the various Java EE 7 specifications for building robust and secure enterprise appl

11β-Hydroxysteroid dehydrogenase type 1 contributes to the balance between 7-keto- and 7-hydroxy-oxysterols in vivo.

Science.gov (United States)

Mitić, Tijana; Shave, Steven; Semjonous, Nina; McNae, Iain; Cobice, Diego F; Lavery, Gareth G; Webster, Scott P; Hadoke, Patrick W F; Walker, Brian R; Andrew, Ruth

2013-07-01

11β-Hydroxysteroid dehydrogenase 1 (11βHSD1; EC 1.1.1.146) generates active glucocorticoids from inert 11-keto metabolites. However, it can also metabolize alternative substrates, including 7β-hydroxy- and 7-keto-cholesterol (7βOHC, 7KC). This has been demonstrated in vitro but its consequences in vivo are uncertain. We used genetically modified mice to investigate the contribution of 11βHSD1 to the balance of circulating levels of 7KC and 7βOHC in vivo, and dissected in vitro the kinetics of the interactions between oxysterols and glucocorticoids for metabolism by the mouse enzyme. Circulating levels of 7KC and 7βOHC in mice were 91.3±22.3 and 22.6±5.7 nM respectively, increasing to 1240±220 and 406±39 nM in ApoE(-/-) mice receiving atherogenic western diet. Disruption of 11βHSD1 in mice increased (p<0.05) the 7KC/7βOHC ratio in plasma (by 20%) and also in isolated microsomes (2 fold). The 7KC/7βOHC ratio was similarly increased when NADPH generation was restricted by disruption of hexose-6-phosphate dehydrogenase. Reduction and oxidation of 7-oxysterols by murine 11βHSD1 proceeded more slowly and substrate affinity was lower than for glucocorticoids. in vitro 7βOHC was a competitive inhibitor of oxidation of corticosterone (Ki=0.9 μM), whereas 7KC only weakly inhibited reduction of 11-dehydrocorticosterone. However, supplementation of 7-oxysterols in cultured cells, secondary to cholesterol loading, preferentially slowed reduction of glucocorticoids, rather than oxidation. Thus, in mouse, 11βHSD1 influenced the abundance and balance of circulating and tissue levels of 7βOHC and 7KC, promoting reduction of 7KC. In health, 7-oxysterols are unlikely to regulate glucocorticoid metabolism. However, in hyperlipidaemia, 7-oxysterols may inhibit glucocorticoid metabolism and modulate signaling through corticosteroid receptors. Copyright © 2013 Elsevier Inc. All rights reserved.

Tetraspanin 7 (TSPAN7) expression is upregulated in multiple myeloma patients and inhibits myeloma tumour development in vivo

Energy Technology Data Exchange (ETDEWEB)

Cheong, Chee Man [Myeloma Research Laboratory, School of Medical Sciences, University of Adelaide, and South Australian Health and Medical Research Institute (SAHMRI), Adelaide 5000, SA (Australia); Chow, Annie W.S. [Myeloma Research Laboratory, School of Medical Sciences, University of Adelaide, and South Australian Health and Medical Research Institute (SAHMRI), Adelaide 5000, SA (Australia); Department of Haematology, SA Pathology, Adelaide 5000, SA (Australia); Fitter, Stephen [Myeloma Research Laboratory, School of Medical Sciences, University of Adelaide, and South Australian Health and Medical Research Institute (SAHMRI), Adelaide 5000, SA (Australia); Hewett, Duncan R. [Myeloma Research Laboratory, School of Medical Sciences, University of Adelaide, and South Australian Health and Medical Research Institute (SAHMRI), Adelaide 5000, SA (Australia); School of Medicine, University of Adelaide, Adelaide 5005, SA (Australia); Martin, Sally K. [Myeloma Research Laboratory, School of Medical Sciences, University of Adelaide, and South Australian Health and Medical Research Institute (SAHMRI), Adelaide 5000, SA (Australia); Department of Haematology, SA Pathology, Adelaide 5000, SA (Australia); School of Medicine, University of Adelaide, Adelaide 5005, SA (Australia); Williams, Sharon A. [Myeloma Research Laboratory, School of Medical Sciences, University of Adelaide, and South Australian Health and Medical Research Institute (SAHMRI), Adelaide 5000, SA (Australia); To, L. Bik [Department of Haematology, SA Pathology, Adelaide 5000, SA (Australia); and others

2015-03-01

Background: Increased expression of the tetraspanin TSPAN7 has been observed in a number of cancers; however, it is unclear how TSPAN7 plays a role in cancer progression. Methods: We investigated the expression of TSPAN7 in the haematological malignancy multiple myleoma (MM) and assessed the consequences of TSPAN7 expression in the adhesion, migration and growth of MM plasma cells (PC) in vitro and in bone marrow (BM) homing and tumour growth in vivo. Finally, we characterised the association of TSPAN7 with cell surface partner molecules in vitro. Results: TSPAN7 was found to be highly expressed at the RNA and protein level in CD138{sup +} MM PC from approximately 50% of MM patients. TSPAN7 overexpression in the murine myeloma cell line 5TGM1 significantly reduced tumour burden in 5TGM1/KaLwRij mice 4 weeks after intravenous adminstration of 5TGM1 cells. While TSPAN7 overexpression did not affect cell proliferation in vitro, TSPAN7 increased 5TGM1 cell adhesion to BM stromal cells and transendothelial migration. In addition, TSPAN7 was found to associate with the molecular chaperone calnexin on the cell surface. Conclusion: These results suggest that elevated TSPAN7 may be associated with better outcomes for up to 50% of MM patients. - Highlights: • TSPAN7 expression is upregulated in newly-diagnosed patients with active multiple myeloma. • Overexpression of TSPAN7 inhibits myeloma tumour development in vivo. • TSPAN7 interacts with calnexin at the plasma membrane in a myeloma cell line.

Effects of MgO on short and long term stabilities in water of R7T7 and M7 nuclear waste glasses

Energy Technology Data Exchange (ETDEWEB)

Advocat, T; Vernaz, E; Dussossoy, J [CEA Centre d` Etudes de la Vallee du Rhone, 30 - Marcoule (France). Dept. des Procedes de Retraitement; Crovisier, J L [Centre National de la Recherche Scientifique (CNRS), 67 - Strasbourg (France)

1993-12-31

Magnesium oxide was added to the standard R7T7 and M7 glass compositions developed for containment of fission product solutions. M7 differs from the R7T7 reference glass mainly by a larger proportion of network modifier elements and a correspondingly lower proportion of glass network forming elements. The percentage of fission products, simulated in this study by inactive elements, was the same in both cases. Increasing the MgO content of the glass compositions by 2 to 5 wt% resulted in significant variations in the aqueous leaching resistance at 90 and 100 deg C. Experimental findings demonstrated that the initial dissolution rate measured at 100 deg C in a Soxhlet apparatus was proportional to the MgO content and inversely proportional to the network former content (mainly SiO2). This was confirmed by a glass hydration model based on the thermodynamic stability of the glass matrix components. Aqueous corrosion tests were also conducted at 90 deg C under static conditions at various SA/V ratios to simulate the progress of the reaction. Under these conditions, the glass dissolution rate diminished more slowly in time when the initial magnesium content was high and the network former content was low. This may be due primarily to a variation in the glass silica solubility limit related to the glass composition; it may also be related to the formation of secondary silica and magnesia alteration products controlling the glass dissolution reaction affinity.

Influences of H on the Adsorption of a Single Ag Atom on Si(111-7 × 7 Surface

Directory of Open Access Journals (Sweden)

Lin Xiu-Zhu

2009-01-01

Full Text Available Abstract The adsorption of a single Ag atom on both clear Si(111-7 × 7 and 19 hydrogen terminated Si(111-7 × 7 (hereafter referred as 19H-Si(111-7 × 7 surfaces has been investigated using first-principles calculations. The results indicated that the pre-adsorbed H on Si surface altered the surface electronic properties of Si and influenced the adsorption properties of Ag atom on the H terminated Si surface (e.g., adsorption site and bonding properties. Difference charge density data indicated that covalent bond is formed between adsorbed Ag and H atoms on 19H-Si(111-7 × 7 surface, which increases the adsorption energy of Ag atom on Si surface.

New metal-organic frameworks of [M(C6H5O7)(C6H6O7)(C6H7O7)(H2O)] . H2O (M=La, Ce) and [Ce2(C2O4)(C6H6O7)2] . 4H2O

International Nuclear Information System (INIS)

Weng Shengfeng; Wang, Yun-Hsin; Lee, Chi-Shen

2012-01-01

Two novel materials, [M(C 6 H 5 O 7 )(C 6 H 6 O 7 )(C 6 H 7 O 7 )(H 2 O)] . H 2 O (M=La(1a), Ce(1b)) and [Ce 2 (C 2 O 4 )(C 6 H 6 O 7 ) 2 ] . 4H 2 O (2), with a metal-organic framework (MOF) were prepared with hydrothermal reactions and characterized with photoluminescence, magnetic susceptibility, thermogravimetric analysis and X-ray powder diffraction in situ. The crystal structures were determined by single-crystal X-ray diffraction. Compound 1 crystallized in triclinic space group P1-bar (No. 2); compound 2 crystallized in monoclinic space group P2 1 /c (No. 14). The structure of 1 is built from a 1D MOF, composed of deprotonated citric ligands of three kinds. Compound 2 contains a 2D MOF structure consisting of citrate and oxalate ligands; the oxalate ligand arose from the decomposition in situ of citric acid in the presence of Cu II ions. Photoluminescence spectra of compounds 1b and 2 revealed transitions between the 5d 1 excited state and two levels of the 4f 1 ground state ( 2 F 5/2 and 2 F 7/2 ). Compounds 1b and 2 containing Ce III ion exhibit a paramagnetic property with weak antiferromagnetic interactions between the two adjacent magnetic centers. - Graphical Abstract: [M(C 6 H 5 O 7 )(C 6 H 6 O 7 )(C 6 H 7 O 7 )(H 2 O)] . H 2 O (M=La(1a), Ce(1b)) and [Ce 2 (C 2 O 4 )(C 6 H 6 O 7 ) 2 ] . 4H 2 O (2)—with 1D and 2D structures were synthesized and characterized. Highlights: ► Two MOF – [M(C 6 H 5 O 7 )(C 6 H 6 O 7 )(C 6 H 7 O 7 )(H 2 O)] . H 2 O (M=La(1a), Ce(1b)) and [Ce 2 (C 2 O 4 )(C 6 H 6 O 7 ) 2 ] . 4H 2 O (2) – with 1D and 2D structures. ► The adjacent chains of the 1D framework were correlated with each other through an oxalate ligand to form a 2D layer structure. ► The source of the oxalate ligand was the decomposition in situ of citric acid oxidized in the presence of Cu II ions.

Downregulation of SLC7A7 Triggers an Inflammatory Phenotype in Human Macrophages and Airway Epithelial Cells

Directory of Open Access Journals (Sweden)

Bianca Maria Rotoli

2018-03-01

Full Text Available Lysinuric protein intolerance (LPI is a recessively inherited aminoaciduria caused by mutations of SLC7A7, the gene encoding y+LAT1 light chain of system y+L for cationic amino acid transport. The pathogenesis of LPI is still unknown. In this study, we have utilized a gene silencing approach in macrophages and airway epithelial cells to investigate whether complications affecting lung and immune system are directly ascribable to the lack of SLC7A7 or, rather, mediated by an abnormal accumulation of arginine in mutated cells. When SLC7A7/y+LAT1 was silenced in human THP-1 macrophages and A549 airway epithelial cells by means of short interference RNA (siRNA, a significant induction of the expression and release of the inflammatory mediators IL1β and TNFα was observed, no matter the intracellular arginine availability. This effect was mainly regulated at transcriptional level through the activation of NFκB signaling pathway. Moreover, since respiratory epithelial cells are the important sources of chemokines in response to pro-inflammatory stimuli, the effect of IL1β has been addressed on SLC7A7 silenced A549 cells. Results obtained indicated that the downregulation of SLC7A7/y+LAT1 markedly strengthened the stimulatory effect of the cytokine on CCL5/RANTES expression and release without affecting the levels of CXCL8/IL8. Consistently, also the conditioned medium of silenced THP-1 macrophages activated airway epithelial cells in terms of CCL5/RANTES expression due to the presence of elevated amount of proinflammatory cytokines. In conclusion, our results point to a novel thus far unknown function of SLC7A7/y+LAT1, that, under physiological conditions, besides transporting arginine, may act as a brake to restrain inflammation.

30 CFR 7.46 - Impact test.

Science.gov (United States)

2010-07-01

... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Impact test. 7.46 Section 7.46 Mineral... MINING PRODUCTS TESTING BY APPLICANT OR THIRD PARTY Battery Assemblies § 7.46 Impact test. (a) Test... individual cells. At the test temperature range of 65 °F -80 °F (18.3 °C-26.7 °C), apply a dynamic force of...

Refolding in high hydrostatic pressure of recombinant proteins from inclusion bodies in Escherichia Coli; Renaturacao em altas pressoes hidrostaticas de proteinas recombinantes agregadas em corpos de inclusao produzidos em Escherichia Coli

Energy Technology Data Exchange (ETDEWEB)

Balduino, Keli Nunes

2009-07-01

The expression of proteins as inclusion bodies in bacteria is a widely used alternative for production of recombinant protein. However, the aggregation is a problem often encountered during refolding of these proteins. High hydrostatic pressure are able to solubilise the inclusion bodies in the presence of low concentrations of denaturant reagents, encouraging refolding protein with high efficiency and reduce costs. This work aims to refolding of recombinant proteins expressed in Escherichia coli from inclusion bodies using high hydrostatic pressure. Three toxins, all featuring five or more disulfide bonds were studied: NXH8, Natterin 2 and Bothropstoxin 1. Suspensions of inclusion bodies of the three proteins were pressurized to 2000 bars for 16 hours. The buffers were optimized for refolding of the three proteins. The buffer used in the refolding of NXH8 was 50 mM Tris HCl, pH 9.0 with proportion of 1GSH: 4GSSG at a concentration of 6 mM and 2 M GdnHCl. Inclusion bodies were used in O.D. (A600nm) of 0.5. After refolding process, dialysis was performed at pH 7.0. The final yield of obtaining soluble NXH8 was 40% (28,6 mg of soluble NXH8/L of culture medium). The refolding of Bothropstoxin 1 was obtained in refolding buffer of Tris HCl 50 mM, pH 7,5 with proportion of 2 GSH: GSSG 3 and concentration of 3 mM and 1 M GdnHCl. Use with a suspension of O.D. (A600nm) of 0.5. The final yield of recovery of Bothropstoxin 1 refolded was 32% (9,2 mg of refolded Bothropstoxin 1/L of culture medium). The refolding of Natterin 2 was performed in the refolding buffer: 20 mM Tris HCl pH 9.0 at a ratio of 2 GSH: 3GSSG and concentration of 10 mM and 1 M GdnHCl and inclusion bodies O.D. (A600nm) of 6.0. The yield of Natterin 2 refolded was 20% (3,7 mg/L of culture medium). Physico-chemical and biological analysis were performed by SDS-PAGE, western blot, scanning electron microscopy, biological tests in vivo and in vitro and structural. The analysis conducted in NXH8 did not show

Low-field spin dynamics of Cr₇Ni and Cr₇Ni-Cu-Cr₇Ni molecular rings as detected by μsR

OpenAIRE

Sanna, S.; Arosio, P.; Bordonali, L.; Adelnia, F.; Mariani, M.; Garlatti, E.; Baines, C.; Amato, A.; Sabareesh, K. P.V.; Timco, G.; Winpenny, R. E.P.; Blundell, S. J.; Lascialfari, A.

2017-01-01

Muon spin rotation measurements were used to investigate the spin dynamics of heterometallic Cr7Ni and Cr7Ni-Cu-Cr7Ni molecular clusters. In Cr7Ni the magnetic ions are arranged in a quasiplanar ring and interact via an antiferromagnetic exchange coupling constant J, while Cr7Ni-Cu-Cr7Ni is composed of two Cr7Ni linked by a bridging moiety containing one Cu ion, that induces an inter-ring ferromagnetic interaction J ≪ J. The longitudinal muon relaxation rate λ collected at low magnetic fields...

Targeting B7x and B7-H3 as New Immunotherapies for Prostate Cancer

Science.gov (United States)

2017-11-01

prostate   cancer  and  other   cancers .   15. SUBJECT TERMS B7x, B7-H3, HHLA2, TMIGD2, Receptors , Immune Checkpoint, Prostate Cancer , Monoclonal...H3,  HHLA2,  TMIGD2,   Receptors ,  Immune  Checkpoint,   Prostate   Cancer ,   Monoclonal  Antibodies,  Crystal  Structure,  Immunotherapy,  T  Cells... prostate   cancer  immunotherapy.       Unlike  B7x  and  B7-­H3  whose   receptors  have  not  been  found  yet,  we  have   quickly  discovered  two

Skeleton polyhedral rearrangements of 8-alkyl-7,9-dicarba-nido-undecaborate(-1) anion to 11-alkyl-2,7-dicarba-nido-undecaborane(13) and to 9-alkyl-7,8-dicarba-nido-undecaborate(-1) anion. Molecular structure of 2,7-Me2-11-PhCH2-2,7-C2B9H10

International Nuclear Information System (INIS)

Zakharkin, K.I.; Zhigareva, G.G.; Antonovich, V.A.; Yanovskij, A.I.; Struchkov, Yu.T.

1986-01-01

Using the methods of 1 H and 11 B NMR-spectroscopy and X-ray diffraction analysis skeleton polyhedral rearrangements of carboranes are studied. During protonation of anion 8-R-7, 9-R' 2 -7, 9-C 2 B 9 H 9 - the skeleton polyhedral rearrangement in the series of nido-carboranes, resulting in 2,7-R' 2 -11-R-2, 7-C 2 B 9 H 10 (R=Me, PhCH 2 , R'=H, Me) is observed. The rearrangement is reversible. On the detachment of two protons from 2,7-Me 2 -11-PhCH 2 -2,7-C 2 B 9 H 10 and during subsequent protonation the skeleton polyhedral rearrangement with the formation of anion 9-PhCH 2 -7,8-Me 2 -7,8-C 2 B 9 H 9 - takes place

Novel CD7-specific nanobody-based immunotoxins potently enhanced apoptosis of CD7-positive malignant cells.

Science.gov (United States)

Tang, Jinle; Li, Jialu; Zhu, Xuejun; Yu, Yuan; Chen, Dan; Yuan, Lei; Gu, Zhenyang; Zhang, Xingding; Qi, Lin; Gong, Zhishu; Jiang, Pengjun; Yu, Juhua; Meng, Huimin; An, Gangli; Zheng, Huyong; Yang, Lin

2016-06-07

Various CD7-targeting immunotoxins have been tested for its potential in treating CD7+ malignant patients but none of those immunotoxins was approved clinically because of lacking enough efficacy and safety. Here we successfully constructed the monovalent and bivalent CD7 nanobody-based immunotoxins PG001 and PG002, both conjugated with a truncated derivative of Pseudomonas exotoxin A respectively. The prokaryotic system expressed immunotoxins not only maintained their binding specificity for CD7-positive cells with a Kd of 16.74 nM and 3.6 nM for PG001 and PG002 respectively, but also efficiently promoted antigen-restricted apoptosis of the CD7-positive leukemia cell lines Jurkat and CEM, and primary T-cell acute lymphoblastic leukemia (T-ALL) and acute myeloid leukemia (AML) cells with an in vitro cytotoxic activity (EC50) in the range of 23-30 pM for PG002. In NOD/SCID mice transplanted with CEM cells, PG001 and PG002 prevented engraftment of the cells and markedly prolonged mouse survival. Owing to the efficient antigen-restricted anti-leukemic activity of PG002, this CD7 nanobody-based immunotoxin exhibited a superior anti-CD7 positive malignancies activity than previously reported immunotoxins, and may represent a promising therapeutic strategy in treating CD7-positive leukemia and lymphoma, which still remain a significant clinical challenge.

Cross-section of the reaction {sup 7}Li(p,n){sup 7}Be close to the threshold

Energy Technology Data Exchange (ETDEWEB)

Shorin, V S [Institute of Physics and Power Engineering, Obninsk (Russian Federation)

1997-06-01

The status of data on the cross-section of the reaction {sup 7}Li(p,n){sup 7}Be close to the threshold is reviewed. On the basis of recent data on the cross-section of the inverse reaction {sup 7}Be(n,p){sup 7}Li and certain theoretical models, an evaluation is performed of the total cross-section of the {sup 7}Li(p,n)-reaction in the proton energy region up to 2 MeV. (author). 16 refs, 1 fig., 1 tab.

UEL 7: nova cultivar de Dendrobium UEL 7: a new Dendrobium cultivar

Directory of Open Access Journals (Sweden)

Ricardo Tadeu de Faria

2011-09-01

Full Text Available UEL 7 é uma cultivar de Dendrobium nobile desenvolvida no Departamento de Agronomia da Universidade Estadual de Londrina resultante do cruzamento das plantas matrizes D3 x D8 e selecionada pela coloração das flores amarelas e com largura e comprimento semelhantes (7,0 cm, formando praticamente um círculo. As plantas foram polinizadas artificialmente, e as sementes semeadas em meio de cultura in vitro. A cultivar apresenta flores de coloração amarela, labelo com o centro marrom escuro e o pseudobulbo apresenta em mé dia 11 flores. As flores têm durabilidade de aproximadamente 30 dias.UEL 7 is a Dendrobium nobile's cultivar developed at The Department of Agronomy of Universidade Estadual de Londrina and is the result of the crossing between D3 x D8 and selected for its yellow flowers and format with similar width and length (7.0 cm, almost forming a circle. The plants were pollinated artificially, and the seeds sowed in growth media in vitro. The cultivar presents flowers of yellow coloration, labelo with the center dark brown color and the pseudobulb presents 11 flowers on average. The flowers durability is about 30 days.

Copper interactions in TlCu7S4 and TlCu7Se4

International Nuclear Information System (INIS)

Noren, L.; Delaplane, R.G.; Berger, R.

1999-01-01

Complete text of publication follows. The copper chalcogenides ACu 7 S 4 (A=NH 4 + , Tl + , Rb + ) are quasi-one-dimensional metals at ambient and higher temperatures which is due to the high mobility of copper in these structures. TlCu 7 S 4 and TlCu 7 Se 4 are isostructural compounds, space group I4/m, which can be described on the basis of a TlX 8 cube with two different Cu sites, Cu(1) and Cu(2). Cu(2)-Cu(2) zigzag chains run along the c axis with only 3/4 occupation of the Cu(2) sites. However, these two compounds differ in behaviour on cooling. The sulphide shows a polymorphic first-order transition to the CsAg 7 S 4 type (P4/n) owing to ordering of the vacancies in the Cu(2)-Cu(2) chains. In order to study the nature of the Cu(2) order/disorder in the two title compounds, a series of neutron diffraction measurements (both Bragg and diffuse scattering) were made at several temperatures from 40 to 713 K on the instrument SLAD at Studsvik. The structure at each temperature was modelled using RMC techniques. The resulting configuration show that as the temperature increases, there is a marked increase in the mobility of the Cu atoms in the Cu(2)-Cu(2) chains for TlCu 7 S 4 but not for TlCu 7 Se 4 . This is due to the initial difference in the Cu(2)-Cu(2) distances, only 2.2A for the thiocuprate, but 2.7A in the selenocuprate which explains the relative ease for Cu(2) ordering in the latter case. (author)

High yield expression and purification of equilibrative nucleoside transporter 7 (ENT7) from Arabidopsis thaliana.

Science.gov (United States)

Girke, Christopher; Arutyunova, Elena; Syed, Maria; Traub, Michaela; Möhlmann, Torsten; Lemieux, M Joanne

2015-09-01

Equilibrative nucleoside transporters (ENTs) facilitate the import of nucleosides and their analogs into cells in a bidirectional, non-concentrative manner. However, in contrast to their name, most characterized plant ENTs act in a concentrative manner. A direct characterization of any ENT protein has been hindered due to difficulties in overexpression and obtaining pure recombinant protein. The equilibrative nucleoside transporter 7 from Arabidopsis thaliana (AtENT7) was expressed in Xenopus laevis oocytes to assess mechanism of substrate uptake. Recombinant protein fused to enhanced green fluorescent protein (eGFP) was expressed in Pichia pastoris to characterize its oligomeric state by gel filtration and substrate binding by microscale thermophoresis (MST). AtENT7 expressed in X. laevis oocytes works as a classic equilibrative transporter. The expression of AtENT7-eGFP in the P. pastoris system yielded milligram amounts of pure protein that exists as stable homodimers. The concentration dependent binding of purine and pyrimidine nucleosides to the purified recombinant protein, assessed by MST, confirmed that AtENT7-eGFP is properly folded. For the first time the binding of nucleobases was observed for AtENT7. The availability of pure recombinant AtENT7 will permit detailed kinetic and structural studies of this unique member of the ENT family and, given the functional similarity to mammalian ENTs, will serve as a good model for understanding the structural basis of translocation mechanism for the family. Copyright © 2015 Elsevier B.V. All rights reserved.

Low Temperature Scanning Force Microscopy of the Si(111)-( 7x7) Surface

International Nuclear Information System (INIS)

Lantz, M. A.; Hug, H. J.; Schendel, P. J. A. van; Hoffmann, R.; Martin, S.; Baratoff, A.; Abdurixit, A.; Guentherodt, H.-J.; Gerber, Ch.

2000-01-01

A low temperature scanning force microscope (SFM) operating in a dynamic mode in ultrahigh vacuum was used to study the Si(111)-(7x7) surface at 7.2 K. Not only the twelve adatoms but also the six rest atoms of the unit cell are clearly resolved for the first time with SFM. In addition, the first measurements of the short range chemical bonding forces above specific atomic sites are presented. The data are in good agreement with first principles computations and indicate that the nearest atoms in the tip and sample relax significantly when the tip is within a few Angstrom of the surface. (c) 2000 The American Physical Society

The total angular moment selectivity in 7Li(α, α) 7Li(4.63 MeV, 7/2-) reaction at Eα = 27.2 MeV

International Nuclear Information System (INIS)

Dmitrenko, V.N.; Kozyr', Yu.E.

1995-01-01

The DWBA calculation of tensor polarisation of residual nuclei for direct inelastic scattering 7 Li(α, α) 7 Li(4.63 MeV, 7/2 - ) gives the lest approximation to experimental data at selected total angular moment and parity values J π 13/2 + . The microscopic coupled channel calculation also predicts a significant role of total angular moment states with J ≥ 13/2. at E α 27.2 MeV

Purine biosynthesis in L1210 leukemia cells is inhibited by 7-hydroxymethotrexate (7-OH-MTX) polyglutamates (PGS)

International Nuclear Information System (INIS)

Seither, R.L.; Matherly, L.H.; Goldman, I.D.

1986-01-01

The biochemical basis for 7-OH-MTX cytotoxicity was examined in L1210 tumor cells. Cells were exposed to 100 μM 7-OH-MTX (approx. 50% growth inhibition) or 10 μM methotrexate (MTX) (approx. 95% growth inhibition) for 6 hrs to allow high levels of PGS to accumulate. Dihydrofolate reductase (DHFR) activity was assessed by dihydrofolate (FH 2 ) pools labeled with 5-formyl-[ 3 H]-tetrahydrofolate (5μM) or 3 H-folic acid (1 μM). FH 2 was not elevated above control levels in 7-OH-MTX treated cells, in contrast to MTX treated cells in which FH 2 increased 4- to 7-fold. 3 H-Deoxyuridine incorporation into DNA was not inhibited in cells containing high levels (11.5 nmol/g dry wt.) of 7-OH-MTX tetraglutamate (7-OH-4-NH 2 -10-CH 3 -PteGlu 4 ), well in excess of the DHFR-binding capacity (7.3 +/- 0.9 nmol/g), indicating a normal rate of thymidylate synthesis. Although small amounts of 7-OH-MTX and its PGS were bound to DHFR in L1210 cells, as assessed by gel filtration, there was evidence for the preferential binding of 7-OH-MTX tetraglutamate. In all cases this was well below the DHFR binding capacity, consistent with normal rates of deoxyuridine metabolism and FH 2 levels in the cell. Incorporation of 14 C-formate (60 min) into thymidylate and amino acids was unaffected by 7-OH-MTX, yet incorporation into purines was inhibited over 50%, supporting a block(s) in de novo purine biosynthesis

Automating with SIMATIC S7-1500 configuring, programming and testing with STEP 7 Professional

CERN Document Server

Berger, Hans

2014-01-01

With many innovations, the SIMATIC S7-1500 programmable logic controller (PLC) sets new standards in productivity and efficiency in control technology. By its outstanding system performance and with PROFINET as the standard interface, it ensures extremely short system response times and the highest control quality with a maximum of flexibility for most demanding automation tasks. The engineering software STEP 7 Professional operates inside TIA Portal, a user interface that is designed for intuitive operation. Functionality includes all aspects of Automation: from the configuration of the controllers via the programming in the IEC languages ¿¿LAD, FBD, STL, and SCL up to the program test. In the book, the hardware components of the automation system S7-1500 are presented including the description of their configuration and parameterization. A comprehensive introduction into STEP 7 Professional illustrates the basics of programming and troubleshooting. Beginners learn the basics of automation with Simatic...

The Co-III-C bond in (1-thia-4,7-diazacyclodecyl-kappa N-3(4),N-7,C-10)(1,4,7-triazacyclononane-kappa N-3(1),N-4,N-7)-cobalt(III) dithionate hydrate

DEFF Research Database (Denmark)

Harris, Pernille; Kofod, P.; Song, Y.S.

2003-01-01

In the title compound, [Co(C6H15N3)(C7H15N2S)]S2O6.H2O, the Co-C bond distance is 1.9930 (13) Angstrom, which is shorter than for related compounds with the linear 1,6-diamino-3-thiahexan-4-ide anion in place of the macrocyclic 1-thia-4,7-diazacyclodecan-8-ide anion. The coordinated carbanion pro...... produces an elongation of 0.102 (7) Angstrom of the Co-N bond to the 1,4,7-triazacyclononane N atom in the trans position. This relatively small trans influence is presumably a result of the triamine ligand forming strong bonds to the Co-III atom....

Si(111)-7 x 7: First-principles study of dynamics

International Nuclear Information System (INIS)

Stich, I.; Kohanoff, J.; Terakura, K.

1995-12-01

We present a large-scale fully ab initio molecular dynamics study of dynamical properties of the Takayanagi reconstructed Si(111)-7 x 7 surface. The simulation reproduces well the experimentally determined features of the phonon spectra and clarifies their nature and origin. Correlations are found between these dynamical properties and elements of the local electronic structure of the adatom dangling bonds. We find evidence for important anharmonic effects of below room temperature. Use of non-traditional signal-processing methods allows for a considerable insight into the details of the dynamics from a short-duration molecular dynamics trajectory. Results of this analysis significantly extend/modify the results of the previous studies based on more simplified models. (author). 29 refs, 12 figs, 1 tab

7 CFR 767.1 - Introduction.

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2010-01-01

... SPECIAL PROGRAMS INVENTORY PROPERTY MANAGEMENT Overview § 767.1 Introduction. (a) Purpose. This part... 7 Agriculture 7 2010-01-01 2010-01-01 false Introduction. 767.1 Section 767.1 Agriculture... financial interest. ...

TMAP7 User Manual

International Nuclear Information System (INIS)

Longhurst, Glen R.

2008-01-01

The TMAP Code was written at the Idaho National Engineering and Environmental Laboratory by Brad Merrill and James Jones in the late 1980s as a tool for safety analysis of systems involving tritium. Since then it was upgraded to TMAP4 and has been used in numerous applications including experiments supporting fusion safety, predictions for advanced systems such as the International Thermonuclear Experimental Reactor (ITER), and estimates involving tritium production technologies. Its further upgrade to TMAP2000 and now to TMAP7 was accomplished in response to several needs. TMAP and TMAP4 had the capacity to deal with only a single trap for diffusing gaseous species in solid structures. TMAP7 includes up to three separate traps and up to 10 diffusing species. The original code had difficulty dealing with heteronuclear molecule formation such as HD and DT under solution-law dependent diffusion boundary conditions. That difficulty has been overcome. TMAP7 automatically generates heteronuclear molecular partial pressures when solubilities and partial pressures of the homonuclear molecular species are provided for law-dependent diffusion boundary conditions. A further sophistication is the addition of non-diffusing surface species. Atoms such as oxygen or nitrogen or formation and decay or combination of hydroxyl radicals on metal surfaces are sometimes important in reactions with diffusing hydrogen isotopes but do not themselves diffuse appreciably in the material. TMAP7 will accommodate up to 30 such surface species, allowing the user to specify relationships between those surface concentrations and partial pressures of gaseous species above the surfaces or to form them dynamically by combining diffusion species or other surface species. Additionally, TMAP7 allows the user to include a surface binding energy and an adsorption barrier energy. The code includes asymmetrical diffusion between the surface sites and regular diffusion sites in the bulk. All of the

Lower placental growth factor and higher free β-hCG and PAPP-A levels in the fetal circulation of near-term pregnancies complicated with severe preeclampsia.

Science.gov (United States)

Paredes, Verónica; Espinoza-Caicedo, Jasson A; Salazar-Pousada, Danny; Escobar, Gustavo S; Pérez-López, Faustino R; Chedraui, Peter

2017-01-01

An imbalance between anti- and angiogenic factors during early placentation is key for the development of preeclampsia. Nevertheless, the majority of studies addressing this issue relate to maternal blood and not the fetal circulation. To measure placental growth factor (PlGF), free beta human chorionic gonadotropin (β-hCG), and pregnancy-associated plasma protein-A (PAPP-A) levels in the fetal circulation of near-term pregnancies complicated with severe preeclampsia (n = 20), and their controls matched for parity, and maternal and gestational age. Upon delivery, a blood sample was withdrawn from the umbilical artery and vein of each case and its control in order to measure the proposed analytes using direct fluoroimmunoassay. Preeclampsia cases showed significantly lower median PlGF levels in fetal circulation as compared to controls (25.2 versus 36.9 and 23.6 versus 33.9 pg/mL, artery and vein, respectively, p < 0.05). Contrarily, cases displayed higher concentrations of PAPP-A (1024.0 versus 720.9 [median] and 1027.0 ± 298.4 versus 690.3 ± 401.9 mIU/L, artery and vein, respectively, p < 0.05), and free β-hCG (mean: 33.9 ± 4.3 versus 17.2 ± 4.0 and 30.1 ± 5.2 versus 13.7 ± 3.3 ng/mL, artery, and vein respectively, p < 0.05). Lower PlGF and higher PAPP-A and free β-hCG levels were found in the fetal circulation of near-term severe preeclamptic pregnancies. There is a need for more research in this regard.

7 CFR 932.161 - Reports.

Science.gov (United States)

2010-01-01

... a form provided by the committee, for each week (Sunday through Saturday, or such other 7-day period...; (5) acidified; (6) Spanish olives; (7) Sicilian style olives; (8) Greek style olives; (9) olive oil...

29 CFR 4010.7 - Identifying information.

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2010-07-01

... 29 Labor 9 2010-07-01 2010-07-01 false Identifying information. 4010.7 Section 4010.7 Labor Regulations Relating to Labor (Continued) PENSION BENEFIT GUARANTY CORPORATION CERTAIN REPORTING AND DISCLOSURE REQUIREMENTS ANNUAL FINANCIAL AND ACTUARIAL INFORMATION REPORTING § 4010.7 Identifying information...

RERTR-7 Irradiation Summary Report

Energy Technology Data Exchange (ETDEWEB)

D. M. Perez; M. A. Lillo; G. S. Chang; G. A. Roth; N. E. Woolstenhulme; D. M. Wachs

2011-12-01

The Reduced Enrichment for Research and Test Reactor (RERTR) experiment RERTR-7A, was designed to test several modified fuel designs to target fission densities representative of a peak low enriched uranium (LEU) burnup in excess of 90% U-235 at peak experiment power sufficient to generate a peak surface heat flux of approximately 300 W/cm2. The RERTR-7B experiment was designed as a high power test of 'second generation' dispersion fuels at peak experiment power sufficient to generate a surface heat flux on the order of 230 W/cm2.1 The following report summarizes the life of the RERTR-7A and RERTR-7B experiments through end of irradiation, including as-run neutronic analyses, thermal analyses and hydraulic testing results.

Genome and proteome analysis of 7-7-1, a flagellotropic phage infecting Agrobacterium sp H13-3

Directory of Open Access Journals (Sweden)

Kropinski Andrew M

2012-05-01

Full Text Available Abstract Background The flagellotropic phage 7-7-1 infects motile cells of Agrobacterium sp H13-3 by attaching to and traveling along the rotating flagellar filament to the secondary receptor at the base, where it injects its DNA into the host cell. Here we describe the complete genomic sequence of 69,391 base pairs of this unusual bacteriophage. Methods The sequence of the 7-7-1 genome was determined by pyro(454sequencing to a coverage of 378-fold. It was annotated using MyRAST and a variety of internet resources. The structural proteome was analyzed by SDS-PAGE coupled electrospray ionization-tandem mass spectrometry (MS/MS. Results Sequence annotation and a structural proteome analysis revealed 127 open reading frames, 84 of which are unique. In six cases 7-7-1 proteins showed sequence similarity to proteins from the virulent Burkholderia myovirus BcepB1A. Unique features of the 7-7-1 genome are the physical separation of the genes encoding the small (orf100 and large (orf112 subunits of the DNA packaging complex and the apparent lack of a holin-lysin cassette. Proteomic analysis revealed the presence of 24 structural proteins, five of which were identified as baseplate (orf7, putative tail fibre (orf102, portal (orf113, major capsid (orf115 and tail sheath (orf126 proteins. In the latter case, the N-terminus was removed during capsid maturation, probably by a putative prohead protease (orf114.

Caracterização fenogenotípica da resistência antimicrobiana em Staphylococcus spp. isolados de mastite bovina

Directory of Open Access Journals (Sweden)

Elaine C.L. Mendonça

2012-09-01

Full Text Available A utilização de antibióticos no controle das infecções intramamárias e na eliminação de prováveis fontes de infecção nas fazendas leiteiras se constitui em importante medida de controle. No entanto, o uso inadequado de antibióticos no tratamento da doença pode selecionar cepas resistentes e comprometer a eficiência do tratamento. Bactérias do gênero Staphylococcus spp. estão entre os principais agentes etiológicos da mastite bovina e são freqüentemente resistentes aos antimicrobianos, em especial aos beta-lactâmicos, principalmente por dois mecanismos distintos: a produção da enzima extracelular beta-lactamase, codificada pelo gene blaZ, e a produção de PBP2a ou PBP2´, uma proteína ligante de penicilina de baixa afinidade, codificada pelo gene mecA. A expressão do gene mecA é constitutiva ou induzida por antibióticos betalactâmicos, como a oxacilina e cefoxitina. O gene mecA está inserido no cromossomo através de um elemento genético móvel, denominado cassete estafilocócico cromossômico mec (SCCmec. O presente estudo avaliou o perfil fenogenotípico de resistência aos beta-lactâmicos em 250 isolados de Staphylococcus spp., utilizando os marcadores oxacilina e cefoxitina, de modo a produzir dados que possam contribuir para o conhecimento da resistência antimicrobiana em algumas propriedades leiteiras das regiões Sul-Fluminense e Metropolitana do Estado do Rio de Janeiro com o objetivo de subsidiar a implementação de medidas de controle dessa enfermidade. A avaliação da resistência foi feita a partir de 8 diferentes testes fenotípicos, sendo obtidos 54 perfis. Os testes de difusão em disco simples e ágar screen com oxacilina foram utilizados como "padrão ouro" para os cálculos dos valores de sensibilidade, especificidade e predição por serem preconizados pelo CLSI veterinário. O teste de difusão em disco simples com cefoxitina foi o de melhor desempenho na predição da resistência a

The pentose phosphate pathway in Trypanosoma cruzi: a potential target for the chemotherapy of Chagas disease

Directory of Open Access Journals (Sweden)

Mariana Igoillo-Esteve

2007-12-01

via em resposta à relação NADP/NADPH, é codificada por um número de genes por genoma haplóide e é induzida até 46-vezes por peróxido de hidrogênio em trypomastigotas metacíclicos. Os genes que codificam 6-fosfogluconolactonase, 6-fosfogluconato desidrogenase, transaldolase e transcetolase estão presentes no clone CL Brener como cópia única por genoma haplóide. 6-fosfogluconato desidrogenase é muito instável, mas foi estabilizada introduzindo duas pontes salinas por mutagênese sítio-dirigida. A Ribose-5-fosfato isomerase pertence ao Tipo B; genes que codificam enzimas Tipo A, presentes em mamíferos estão ausentes. A Ribulose-5-fosfato epimerase é codificada por dois genes. As enzimas da via têm um componente citosólico principal, embora várias delas tenham uma localização glicosomal secundária e também, localizações em menor número em outras organelas.

49 CFR 260.7 - Priority consideration.

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2010-10-01

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6 CFR 7.26 - Derivative classification.

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30 CFR 7.85 - Critical characteristics.

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49 CFR 227.7 - Preemptive effect.

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2010-10-01

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12 CFR 7.2010 - Directors' responsibilities.

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46 CFR 161.012-7 - Construction.

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Effect of bone morphogenetic protein-7 (BMP-7 on in vitro survival of caprine preantral follicles Efeito da proteína morfogenética óssea 7 (BMP-7 para a sobrevivência in vitro de folículos pré-antrais caprinos

Directory of Open Access Journals (Sweden)

Valdevane R. Araújo

2010-04-01

Full Text Available This study was conducted in order to verify the effect of different concentrations of BMP-7 in the in vitro survival and development of caprine preantral follicles. Fragments of caprine ovarian cortical tissue were cultured for 1 or 7 days in Minimum Essential Medium (MEM+ supplemented with different concentrations of BMP-7 (1, 10, 50 or 100ng/ml. Non-cultured fragments or those cultured for 1 or 7 days were processed for classical histology and transmission electron microscopy (TEM. Parameters such as follicular survival, activation and growth were evaluated. The results showed that, after 1 or 7 days of culture, the percentage of morphologically normal follicles was significantly reduced in all treatments when compared with fresh control, except at 1ng/ml of BMP-7 for 1 day. In addition, the concentration of 10ng/ml of BMP-7 significantly increases follicular diameter from day 1 to 7 of culture. There was no influence of the other concentrations of BMP-7 regarding to the follicular and oocyte diameter. Ultrastructure studies confirmed follicular integrity after 7 days of culture in 1ng/ml BMP-7. In conclusion, small concentrations of BMP-7 can improve the survival and growth of caprine preantral follicles during in vitro culture.O presente trabalho foi conduzido de modo a se verificar o efeito de diferentes concentrações da BMP-7 no desenvolvimento in vitro de folículos pré-antrais caprinos. Fragmentos de tecido cortical ovariano caprino foram cultivados por 1 ou 7 dias em Minimum Essential Medium (MEM+ suplementado com diferentes concentrações de BMP-7 (1, 10, 50 ou 100ng/ml. Os fragmentos não cultivados ou aqueles cultivados por 1 ou 7 dias foram processados para histologia clássica e microscopia eletrônica de transmissão (TEM, sendo avaliados parâmetros morfológicos indicativos de viabilidade, ativação e crescimento. Os resultados mostraram que o percentual de folículos morfologicamente normais diminuiu significativamente em

file 7

African Journals Online (AJOL)

Methodology. This study was carried out in antenatal clinics of Obafemi Awolowo University Teaching Hospital Complex. It ... adverse effects of malaria in pregnant women clinics of the selected hospitals within the. 7 in endemic areas of the .... like heat and difficulty in installation on NDHS 2008 reports . modern day beds ...

7C's

DEFF Research Database (Denmark)

Christiansen, René Boyer; Buch, Bettina; Petersen, Anne Kristine

2017-01-01

seems to be in opposition to the expected personalised learning pathways, and instead they seem to follow the same, teacher instructed learning path. To bring the 7C’s model alive we suggest that it is taken into consideration that this school discourse plays an important role when planning MOOCs...

Cross-reactivity between avian influenza A (H7N9) virus and divergent H7 subtypic- and heterosubtypic influenza A viruses.

Science.gov (United States)

Guo, Li; Wang, Dayan; Zhou, Hongli; Wu, Chao; Gao, Xin; Xiao, Yan; Ren, Lili; Paranhos-Baccalà, Gláucia; Shu, Yuelong; Jin, Qi; Wang, Jianwei

2016-02-24

The number of human avian H7N9 influenza infections has been increasing in China. Understanding their antigenic and serologic relationships is crucial for developing diagnostic tools and vaccines. Here, we evaluated the cross-reactivities and neutralizing activities among H7 subtype influenza viruses and between H7N9 and heterosubtype influenza A viruses. We found strong cross-reactivities between H7N9 and divergent H7 subtypic viruses, including H7N2, H7N3, and H7N7. Antisera against H7N2, H7N3, and H7N7 could also effectively neutralize two distinct H7N9 strains. Two-way cross-reactivities exist within group 2, including H3 and H4, whereas one-way cross-reactivities were found across other groups, including H1, H10, H9, and H13. Our data indicate that the hemaglutinins from divergent H7 subtypes may facilitate the development of vaccines for distinct H7N9 infections. Moreover, serologic diagnoses for H7N9 infections need to consider possible interference from the cross-reactivity of H7N9 with other subtype influenza viruses.

7 CFR 801.10 - [Reserved

Science.gov (United States)

2010-01-01

... 7 Agriculture 7 2010-01-01 2010-01-01 false [Reserved] 801.10 Section 801.10 Agriculture Regulations of the Department of Agriculture (Continued) GRAIN INSPECTION, PACKERS AND STOCKYARD... FOR GRAIN INSPECTION EQUIPMENT § 801.10 [Reserved] ...

6 CFR 25.7 - Litigation management.

Science.gov (United States)

2010-01-01

... 6 Domestic Security 1 2010-01-01 2010-01-01 false Litigation management. 25.7 Section 25.7 Domestic Security DEPARTMENT OF HOMELAND SECURITY, OFFICE OF THE SECRETARY REGULATIONS TO SUPPORT ANTI-TERRORISM BY FOSTERING EFFECTIVE TECHNOLOGIES § 25.7 Litigation management. (a) Liability for all claims...

32 CFR 1656.7 - Employer responsibilities.

Science.gov (United States)

2010-07-01

... 32 National Defense 6 2010-07-01 2010-07-01 false Employer responsibilities. 1656.7 Section 1656.7... SERVICE § 1656.7 Employer responsibilities. Employers participating in the Alternative Service Program are responsible for: (a) Complying with the employment agreement with Selective Service; (b) Providing a clear...

6 CFR 7.30 - Classification challenges.

Science.gov (United States)

2010-01-01

... 6 Domestic Security 1 2010-01-01 2010-01-01 false Classification challenges. 7.30 Section 7.30... INFORMATION Classified Information § 7.30 Classification challenges. (a) Authorized holders of information... classified are encouraged and expected to challenge the classification status of that information pursuant to...

32 CFR 215.7 - Command relationships.

Science.gov (United States)

2010-07-01

... 32 National Defense 2 2010-07-01 2010-07-01 false Command relationships. 215.7 Section 215.7 National Defense Department of Defense (Continued) OFFICE OF THE SECRETARY OF DEFENSE (CONTINUED) MISCELLANEOUS EMPLOYMENT OF MILITARY RESOURCES IN THE EVENT OF CIVIL DISTURBANCES § 215.7 Command relationships...

23 CFR 924.7 - Program structure.

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2010-04-01

... 23 Highways 1 2010-04-01 2010-04-01 false Program structure. 924.7 Section 924.7 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION HIGHWAY SAFETY HIGHWAY SAFETY IMPROVEMENT PROGRAM § 924.7 Program structure. (a) The HSIP shall include a data-driven SHSP and the resulting...

42 CFR 491.7 - Organizational structure.

Science.gov (United States)

2010-10-01

... 42 Public Health 5 2010-10-01 2010-10-01 false Organizational structure. 491.7 Section 491.7 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES...: Conditions for Certification; and FQHCs Conditions for Coverage § 491.7 Organizational structure. (a) Basic...

49 CFR 80.7 - Application process.

Science.gov (United States)

2010-10-01

... 49 Transportation 1 2010-10-01 2010-10-01 false Application process. 80.7 Section 80.7 Transportation Office of the Secretary of Transportation CREDIT ASSISTANCE FOR SURFACE TRANSPORTATION PROJECTS § 80.7 Application process. (a) Public and private applicants for credit assistance under this part...

49 CFR 27.7 - Discrimination prohibited.

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2010-10-01

... 49 Transportation 1 2010-10-01 2010-10-01 false Discrimination prohibited. 27.7 Section 27.7... OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE General § 27.7 Discrimination prohibited. (a... participation in, be denied the benefits of, or otherwise be subjected to discrimination under any program or...

29 CFR 4245.7 - PBGC address.

Science.gov (United States)

2010-07-01

... 29 Labor 9 2010-07-01 2010-07-01 false PBGC address. 4245.7 Section 4245.7 Labor Regulations Relating to Labor (Continued) PENSION BENEFIT GUARANTY CORPORATION INSOLVENCY, REORGANIZATION, TERMINATION, AND OTHER RULES APPLICABLE TO MULTIEMPLOYER PLANS NOTICE OF INSOLVENCY § 4245.7 PBGC address. See...

Fbw7α and Fbw7γ Collaborate To Shuttle Cyclin E1 into the Nucleolus for Multiubiquitylation

Science.gov (United States)

Bhaskaran, Nimesh; van Drogen, Frank; Ng, Hwee-Fang; Kumar, Raman; Ekholm-Reed, Susanna; Peter, Matthias

2013-01-01

Cyclin E1, an activator of cyclin-dependent kinase 2 (Cdk2) that promotes replicative functions, is normally expressed periodically within the mammalian cell cycle, peaking at the G1-S-phase transition. This periodicity is achieved by E2F-dependent transcription in late G1 and early S phases and by ubiquitin-mediated proteolysis. The ubiquitin ligase that targets phosphorylated cyclin E is SCFFbw7 (also known as SCFCdc4), a member of the cullin ring ligase (CRL) family. Fbw7, a substrate adaptor subunit, is expressed as three splice-variant isoforms with different subcellular distributions: Fbw7α is nucleoplasmic but excluded from the nucleolus, Fbw7β is cytoplasmic, and Fbw7γ is nucleolar. Degradation of cyclin E in vivo requires SCF complexes containing Fbw7α and Fbw7γ, respectively. In vitro reconstitution showed that the role of SCFFbw7α in cyclin E degradation, rather than ubiquitylation, is to serve as a cofactor of the prolyl cis-trans isomerase Pin1 in the isomerization of a noncanonical proline-proline bond in the cyclin E phosphodegron. This isomerization is required for subsequent binding and ubiquitylation by SCFFbw7γ. Here we show that Pin1-mediated isomerization of the cyclin E phosphodegron and subsequent binding to Fbw7γ drive nucleolar localization of cyclin E, where it is ubiquitylated by SCFFbw7γ prior to its degradation by the proteasome. It is possible that this constitutes a mechanism for rapid inactivation of phosphorylated cyclin E by nucleolar sequestration prior to its multiubiquitylation and degradation. PMID:23109421

PrB{sub 7}{sup -}. A praseodymium-doped boron cluster with a Pr{sup II} center coordinated by a doubly aromatic planar η{sup 7}-B{sub 7}{sup 3-} ligand

Energy Technology Data Exchange (ETDEWEB)

Chen, Teng-Teng; Jian, Tian; Wang, Lai-Sheng [Department of Chemistry, Brown University, Providence, RI (United States); Li, Wan-Lu; Chen, Xin; Li, Jun [Department of Chemistry and Key Laboratory of Organic Optoelectronics and Molecular Engineering of Ministry of Education, Tsinghua University, Beijing (China)

2017-06-06

The structure and bonding of a Pr-doped boron cluster (PrB{sub 7}{sup -}) are investigated using photoelectron spectroscopy and quantum chemistry. The adiabatic electron detachment energy of PrB{sub 7}{sup -} is found to be low [1.47(8) eV]. A large energy gap is observed between the first and second detachment features, indicating a highly stable neutral PrB{sub 7}. Global minimum searches and comparison between experiment and theory show that PrB{sub 7}{sup -} has a half-sandwich structure with C{sub 6v} symmetry. Chemical bonding analyses show that PrB{sub 7}{sup -} can be viewed as a Pr{sup II}[η{sup 7}-B{sub 7}{sup 3-}] complex with three unpaired electrons, corresponding to a Pr (4f{sup 2}6s{sup 1}) open-shell configuration. Upon detachment of the 6s electron, the neutral PrB{sub 7} cluster is a highly stable Pr{sup III}[η{sup 7}-B{sub 7}{sup 3-}] complex with Pr in its favorite +3 oxidation state. The B{sub 7}{sup 3-} ligand is found to be highly stable and doubly aromatic with six delocalized π and six delocalized σ electrons and should exist for a series of lanthanide M{sup III}[η{sup 7}-B{sub 7}{sup 3-}] complexes. (copyright 2017 Wiley-VCH Verlag GmbH and Co. KGaA, Weinheim)

CXC chemokine receptor 7 (CXCR7 regulates CXCR4 protein expression and capillary tuft development in mouse kidney.

Directory of Open Access Journals (Sweden)

Sammy Haege

Full Text Available BACKGROUND: The CXCL12/CXCR4 axis is involved in kidney development by regulating formation of the glomerular tuft. Recently, a second CXCL12 receptor was identified and designated CXCR7. Although it is established that CXCR7 regulates heart and brain development in conjunction with CXCL12 and CXCR4, little is known about the influence of CXCR7 on CXCL12 dependent kidney development. METHODOLOGY/PRINCIPAL FINDINGS: We provided analysis of CXCR7 expression and function in the developing mouse kidney. Using in situ hybridization, we identified CXCR7 mRNA in epithelial cells including podocytes at all nephron stages up to the mature glomerulus. CXCL12 mRNA showed a striking overlap with CXCR7 mRNA in epithelial structures. In addition, CXCL12 was detected in stromal cells and the glomerular tuft. Expression of CXCR4 was complementary to that of CXCR7 as it occurred in mesenchymal cells, outgrowing ureteric buds and glomerular endothelial cells but not in podocytes. Kidney examination in CXCR7 null mice revealed ballooning of glomerular capillaries as described earlier for CXCR4 null mice. Moreover, we detected a severe reduction of CXCR4 protein but not CXCR4 mRNA within the glomerular tuft and in the condensed mesenchyme. Malformation of the glomerular tuft in CXCR7 null mice was associated with mesangial cell clumping. CONCLUSIONS/SIGNIFICANCE: We established that there is a similar glomerular pathology in CXCR7 and CXCR4 null embryos. Based on the phenotype and the anatomical organization of the CXCL12/CXCR4/CXCR7 system in the forming glomerulus, we propose that CXCR7 fine-tunes CXCL12/CXCR4 mediated signalling between podocytes and glomerular capillaries.

CXC Chemokine Receptor 7 (CXCR7) Regulates CXCR4 Protein Expression and Capillary Tuft Development in Mouse Kidney

Science.gov (United States)

Haege, Sammy; Mueller, Wiebke; Nietzsche, Sandor; Lupp, Amelie; Mackay, Fabienne; Schulz, Stefan; Stumm, Ralf

2012-01-01

Background The CXCL12/CXCR4 axis is involved in kidney development by regulating formation of the glomerular tuft. Recently, a second CXCL12 receptor was identified and designated CXCR7. Although it is established that CXCR7 regulates heart and brain development in conjunction with CXCL12 and CXCR4, little is known about the influence of CXCR7 on CXCL12 dependent kidney development. Methodology/Principal Findings We provided analysis of CXCR7 expression and function in the developing mouse kidney. Using in situ hybridization, we identified CXCR7 mRNA in epithelial cells including podocytes at all nephron stages up to the mature glomerulus. CXCL12 mRNA showed a striking overlap with CXCR7 mRNA in epithelial structures. In addition, CXCL12 was detected in stromal cells and the glomerular tuft. Expression of CXCR4 was complementary to that of CXCR7 as it occurred in mesenchymal cells, outgrowing ureteric buds and glomerular endothelial cells but not in podocytes. Kidney examination in CXCR7 null mice revealed ballooning of glomerular capillaries as described earlier for CXCR4 null mice. Moreover, we detected a severe reduction of CXCR4 protein but not CXCR4 mRNA within the glomerular tuft and in the condensed mesenchyme. Malformation of the glomerular tuft in CXCR7 null mice was associated with mesangial cell clumping. Conclusions/Significance We established that there is a similar glomerular pathology in CXCR7 and CXCR4 null embryos. Based on the phenotype and the anatomical organization of the CXCL12/CXCR4/CXCR7 system in the forming glomerulus, we propose that CXCR7 fine-tunes CXCL12/CXCR4 mediated signalling between podocytes and glomerular capillaries. PMID:22880115

Heteromeric Kv7.2/7.3 channels differentially regulate action potential initiation and conduction in neocortical myelinated axons.

Science.gov (United States)

Battefeld, Arne; Tran, Baouyen T; Gavrilis, Jason; Cooper, Edward C; Kole, Maarten H P

2014-03-05

Rapid energy-efficient signaling along vertebrate axons is achieved through intricate subcellular arrangements of voltage-gated ion channels and myelination. One recently appreciated example is the tight colocalization of K(v)7 potassium channels and voltage-gated sodium (Na(v)) channels in the axonal initial segment and nodes of Ranvier. The local biophysical properties of these K(v)7 channels and the functional impact of colocalization with Na(v) channels remain poorly understood. Here, we quantitatively examined K(v)7 channels in myelinated axons of rat neocortical pyramidal neurons using high-resolution confocal imaging and patch-clamp recording. K(v)7.2 and 7.3 immunoreactivity steeply increased within the distal two-thirds of the axon initial segment and was mirrored by the conductance density estimates, which increased from ~12 (proximal) to 150 pS μm(-2) (distal). The axonal initial segment and nodal M-currents were similar in voltage dependence and kinetics, carried by K(v)7.2/7.3 heterotetramers, 4% activated at the resting membrane potential and rapidly activated with single-exponential time constants (~15 ms at 28 mV). Experiments and computational modeling showed that while somatodendritic K(v)7 channels are strongly activated by the backpropagating action potential to attenuate the afterdepolarization and repetitive firing, axonal K(v)7 channels are minimally recruited by the forward-propagating action potential. Instead, in nodal domains K(v)7.2/7.3 channels were found to increase Na(v) channel availability and action potential amplitude by stabilizing the resting membrane potential. Thus, K(v)7 clustering near axonal Na(v) channels serves specific and context-dependent roles, both restraining initiation and enhancing conduction of the action potential.

43 CFR 7.32 - Supplemental definitions.

Science.gov (United States)

2010-10-01

... 43 Public Lands: Interior 1 2010-10-01 2010-10-01 false Supplemental definitions. 7.32 Section 7... RESOURCES Department of the Interior Supplemental Regulations § 7.32 Supplemental definitions. For purposes of this subpart, the following definitions will be used: (a) Site of religious or cultural importance...

29 CFR 1960.7 - Financial management.

Science.gov (United States)

2010-07-01

... 29 Labor 9 2010-07-01 2010-07-01 false Financial management. 1960.7 Section 1960.7 Labor... MATTERS Administration § 1960.7 Financial management. (a) The head of each agency shall ensure that the... Official, management officials in charge of each establishment, safety and health officials at all...

10 CFR 40.7 - Employee protection.

Science.gov (United States)

2010-01-01

... protected activities. An employee's engagement in protected activities does not automatically render him or... 10 Energy 1 2010-01-01 2010-01-01 false Employee protection. 40.7 Section 40.7 Energy NUCLEAR REGULATORY COMMISSION DOMESTIC LICENSING OF SOURCE MATERIAL General Provisions § 40.7 Employee protection. (a...

10 CFR 30.7 - Employee protection.

Science.gov (United States)

2010-01-01

... occurs because the employee has engaged in protected activities. An employee's engagement in protected... 10 Energy 1 2010-01-01 2010-01-01 false Employee protection. 30.7 Section 30.7 Energy NUCLEAR... Provisions § 30.7 Employee protection. (a) Discrimination by a Commission licensee, an applicant for a...

14 CFR 157.7 - FAA determinations.

Science.gov (United States)

2010-01-01

... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false FAA determinations. 157.7 Section 157.7... NOTICE OF CONSTRUCTION, ALTERATION, ACTIVATION, AND DEACTIVATION OF AIRPORTS § 157.7 FAA determinations. (a) The FAA will conduct an aeronautical study of an airport proposal and, after consultations with...

12 CFR 408.7 - Environmental information.

Science.gov (United States)

2010-01-01

... 12 Banks and Banking 4 2010-01-01 2010-01-01 false Environmental information. 408.7 Section 408.7 Banks and Banking EXPORT-IMPORT BANK OF THE UNITED STATES PROCEDURES FOR COMPLIANCE WITH THE NATIONAL ENVIRONMENTAL POLICY ACT Eximbank Implementing Procedures § 408.7 Environmental information. Interested persons...

10 CFR 451.7 - Metering requirements.

Science.gov (United States)

2010-01-01

... 10 Energy 3 2010-01-01 2010-01-01 false Metering requirements. 451.7 Section 451.7 Energy DEPARTMENT OF ENERGY ENERGY CONSERVATION RENEWABLE ENERGY PRODUCTION INCENTIVES § 451.7 Metering requirements. The net electric energy generated and sold (kilowatt-hours) by the owner or operator of a qualified...

15 CFR 785.7 - Summary decision.

Science.gov (United States)

2010-01-01

... 15 Commerce and Foreign Trade 2 2010-01-01 2010-01-01 false Summary decision. 785.7 Section 785.7 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) BUREAU OF INDUSTRY AND SECURITY, DEPARTMENT OF COMMERCE ADDITIONAL PROTOCOL REGULATIONS ENFORCEMENT § 785.7 Summary...

24 CFR 203.7 - Commitment process.

Science.gov (United States)

2010-04-01

... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Commitment process. 203.7 Section 203.7 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued... Insurance, and Commitments § 203.7 Commitment process. For single family mortgage programs that are not...

29 CFR 785.7 - Judicial construction.

Science.gov (United States)

2010-07-01

... 29 Labor 3 2010-07-01 2010-07-01 false Judicial construction. 785.7 Section 785.7 Labor Regulations Relating to Labor (Continued) WAGE AND HOUR DIVISION, DEPARTMENT OF LABOR STATEMENTS OF GENERAL... Hours Worked § 785.7 Judicial construction. The United States Supreme Court originally stated that...

Professional Windows Embedded Compact 7

CERN Document Server

Phung, Samuel; Joubert, Thierry; Hall, Mike

2011-01-01

Learn to program an array of customized devices and solutions As a compact, highly efficient, scalable operating system, Windows Embedded Compact 7 (WEC7) is one of the best options for developing a new generation of network-enabled, media-rich, and service-oriented devices. This in-depth resource takes you through the benefits and capabilities of WEC7 so that you can start using this performance development platform today. Divided into several major sections, the book begins with an introduction and then moves on to coverage of OS design, application development, advanced application developm

Windows Phone 7 Made Simple

CERN Document Server

Trautschold, Martin

2011-01-01

With Windows Phone 7, Microsoft has created a completely new smartphone operating system that focuses on allowing users to be productive with their smartphone in new ways, while offering seamless integration and use of Microsoft Office Mobile as well as other productivity apps available in the Microsoft App Store. Windows Phone 7 Made Simple offers a clear, visual, step-by-step approach to using your Windows Phone 7 smartphone, no matter what the manufacturer. Author Jon Westfall is an expert in mobile devices, recognized by Microsoft as a "Most Valuable Professional" with experience

Region 7 Title V facilities

Data.gov (United States)

U.S. Environmental Protection Agency — This web map shows the Region 7 Title V facilities (Clean Air Act major sources), any Class I areas within 300 km of R7 States, and any Tribal areas within 50 miles...

Elevated second-trimester maternal serum β-human chorionic gonadotropin and amniotic fluid alpha-fetoprotein as indicators of adverse obstetric outcomes in fetal Turner syndrome.

Science.gov (United States)

Alvarez-Nava, Francisco; Soto, Marisol; Lanes, Roberto; Pons, Hector; Morales-Machin, Alisandra; Bracho, Ana

2015-12-01

The objective of this study was to determine the ability of biochemical analytes to identify adverse outcomes in pregnancies with Turner syndrome. Maternal serum and amniotic fluid (AF) marker concentrations were measured in 73 singleton pregnancies with Turner syndrome (10-22 weeks of gestation). Fetal Turner syndrome was definitively established by cytogenetic analysis. Two subgroups, fetuses with hydrops fetalis versus fetuses with cystic hygroma, were compared. Receiver operating characteristic curves and relative risk were established for a cut-off multiples of the median ≥3.5 for β-subunit of human chorionic gonadotropin (hCG) or AF alpha-fetoprotein (AFP). Forty-nine (67%) of 73 pregnant women had an abnormal maternal serum. While levels of pregnancy-associated plasma protein-A and free β-subunit (fβ)-hCG were not different to those of the control group, AFP, unconjugated estriol and β-hCG concentrations were significantly different in the study group (P Turner syndrome pregnancies with the highest risk of fetal death. © 2015 Japan Society of Obstetrics and Gynecology.

Impact of type 1 diabetes and glycemic control on fetal aneuploidy biochemical markers

DEFF Research Database (Denmark)

Madsen, Helen Nordahl; Ekelund, Charlotte K; Tørring, Niels

2012-01-01

Objective. To determine the influence of type 1 diabetes mellitus (T1DM) on the first trimester serum markers of fetal aneuploidy; pregnancy-associated plasma protein-A (PAPP-A) and free beta subunit of human chorionic gonadotropin (free β-hCG) and to evaluate the influence of glycemic control...... on these parameters in the pregnant diabetic women. Design. Retrospective study. Setting. Data were extracted from electronic obstetric and laboratory databases at two Danish University Hospitals. Population. Based on 36 415 pregnancies without T1DM (non-T1DM) and 331 pregnancies with T1DM; β-hCG and PAPP-A were...... across the T1DM and non-T1DM groups, respectively. Additionally, the relationship between PAPP-A MoM and HgbA1C was examined in 348 T1DM pregnancies by Spearman's rank correlation. Main outcome measures. Difference in biochemical marker levels between T1DM and non-T1DM. Results. PAPP-A was 0.86 MoM in T1...

Impact of type 1 diabetes and glycemic control on fetal aneuploidy biochemical markers

DEFF Research Database (Denmark)

Madsen, Helen Nordahl; Ekelund, Charlotte Kvist; Tørring, Niels

2012-01-01

Objective: To determine the influence of type 1 diabetes mellitus (T1DM) on the first trimester serum markers of fetal aneuploidy; pregnancy-associated plasma protein-A (PAPP-A) and free beta subunit of human chorionic gonadotropin (free β-hCG) and to evaluate the influence of glycemic control...... on these parameters in the pregnant diabetic women. Design: Retrospective study. Setting: Data were extracted from electronic obstetric and laboratory databases at two Danish University Hospitals. Population: Based on 36,415 pregnancies without T1DM (non-T1DM) and 331 pregnancies with T1DM; β-hCG and PAPP-A were...... across the T1DM and non-T1DM groups, respectively. Additionally, the relation between PAPP-A MoM and HgbA1C was examined in 348 T1DM pregnancies by Spearman’s rank correlation. Main outcome measure: Difference in biochemical marker levels between T1DM and non-T1DM. Results: PAPP-A was 0.86 MoM in T1DM...

Structural models of the human copper P-type ATPases ATP7A and ATP7B

DEFF Research Database (Denmark)

Gourdon, P.; Sitsel, Oleg; Karlsen, J.L.

2012-01-01

The human copper exporters ATP7A and ATP7B contain domains common to all P-type ATPases as well as class-specific features such as six sequential heavy-metal binding domains (HMBD1-HMBD6) and a type-specific constellation of transmembrane helices. Despite the medical significance of ATP7A and ATP7B......, allowing protein-specific properties to be addressed. Furthermore, the mapping of known disease-causing missense mutations indicates that among the heavy-metal binding domains, HMBD5 and HMBD6 are the most crucial for function, thus mimicking the single or dual HMBDs found in most copper-specific P-type...

PRMT7 Preserves Satellite Cell Regenerative Capacity

Directory of Open Access Journals (Sweden)

Roméo Sébastien Blanc

2016-02-01

Full Text Available Regeneration of skeletal muscle requires the continued presence of quiescent muscle stem cells (satellite cells, which become activated in response to injury. Here, we report that whole-body protein arginine methyltransferase PRMT7−/− adult mice and mice conditionally lacking PRMT7 in satellite cells using Pax7-CreERT2 both display a significant reduction in satellite cell function, leading to defects in regenerative capacity upon muscle injury. We show that PRMT7 is preferentially expressed in activated satellite cells and, interestingly, PRMT7-deficient satellite cells undergo cell-cycle arrest and premature cellular senescence. These defects underlie poor satellite cell stem cell capacity to regenerate muscle and self-renew after injury. PRMT7-deficient satellite cells express elevated levels of the CDK inhibitor p21CIP1 and low levels of its repressor, DNMT3b. Restoration of DNMT3b in PRMT7-deficient cells rescues PRMT7-mediated senescence. Our findings define PRMT7 as a regulator of the DNMT3b/p21 axis required to maintain muscle stem cell regenerative capacity.

16 CFR 901.7 - Adverse determination.

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2010-01-01

... 16 Commercial Practices 1 2010-01-01 2010-01-01 false Adverse determination. 901.7 Section 901.7... APPLICATION FOR EXEMPTION FROM THE PROVISIONS OF THE ACT § 901.7 Adverse determination. (a) If, after... of the information before it that it cannot make a favorable determination in connection with the...

14 CFR 415.7 - Payload determination.

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2010-01-01

... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Payload determination. 415.7 Section 415.7... TRANSPORTATION LICENSING LAUNCH LICENSE General § 415.7 Payload determination. A payload determination is... determination. Either a launch license applicant or a payload owner or operator may request a review of its...

40 CFR 1502.7 - Page limits.

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2010-07-01

... 40 Protection of Environment 32 2010-07-01 2010-07-01 false Page limits. 1502.7 Section 1502.7 Protection of Environment COUNCIL ON ENVIRONMENTAL QUALITY ENVIRONMENTAL IMPACT STATEMENT § 1502.7 Page limits. The text of final environmental impact statements (e.g., paragraphs (d) through (g) of § 1502.10...

5 CFR 1655.7 - Interest rate.

Science.gov (United States)

2010-01-01

... 5 Administrative Personnel 3 2010-01-01 2010-01-01 false Interest rate. 1655.7 Section 1655.7 Administrative Personnel FEDERAL RETIREMENT THRIFT INVESTMENT BOARD LOAN PROGRAM § 1655.7 Interest rate. (a... interest rate established by the Department of the Treasury in effect on the date the TSP record keeper...

9 CFR 166.7 - Cooking standards.

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2010-01-01

... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Cooking standards. 166.7 Section 166.7... HEALTH PROTECTION SWINE HEALTH PROTECTION General Provisions § 166.7 Cooking standards. (a) Garbage shall...) Garbage shall be agitated during cooking, except in steam cooking equipment, to ensure that the prescribed...

28 CFR 11.7 - Salary adjustments.

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2010-07-01

... 28 Judicial Administration 1 2010-07-01 2010-07-01 false Salary adjustments. 11.7 Section 11.7 Judicial Administration DEPARTMENT OF JUSTICE DEBT COLLECTION Administration of Debt Collection § 11.7 Salary adjustments. The following debts shall not be subject to the salary offset procedures of § 11.8...

25 CFR 211.7 - Environmental studies.

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2010-04-01

... 25 Indians 1 2010-04-01 2010-04-01 false Environmental studies. 211.7 Section 211.7 Indians BUREAU... DEVELOPMENT General § 211.7 Environmental studies. (a) The Secretary shall ensure that all environmental studies are prepared as required by the National Environmental Policy Act of 1969 (NEPA) and the...

34 CFR 300.7 - Charter school.

Science.gov (United States)

2010-07-01

... 34 Education 2 2010-07-01 2010-07-01 false Charter school. 300.7 Section 300.7 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF SPECIAL EDUCATION AND... DISABILITIES General Definitions Used in This Part § 300.7 Charter school. Charter school has the meaning given...

29 CFR 511.7 - Committee staff.

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2010-07-01

... 29 Labor 3 2010-07-01 2010-07-01 false Committee staff. 511.7 Section 511.7 Labor Regulations Relating to Labor (Continued) WAGE AND HOUR DIVISION, DEPARTMENT OF LABOR REGULATIONS WAGE ORDER PROCEDURE FOR AMERICAN SAMOA § 511.7 Committee staff. Each industry committee will be furnished a lawyer, to...

30 CFR 7.72 - New technology.

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2010-07-01

... 30 Mineral Resources 1 2010-07-01 2010-07-01 false New technology. 7.72 Section 7.72 Mineral... MINING PRODUCTS TESTING BY APPLICANT OR THIRD PARTY Multiple-Shot Blasting Units § 7.72 New technology. MSHA may approve a blasting unit that incorporates technology for which the requirements of this...

30 CFR 7.411 - New technology.

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2010-07-01

... 30 Mineral Resources 1 2010-07-01 2010-07-01 false New technology. 7.411 Section 7.411 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR TESTING, EVALUATION, AND APPROVAL OF... Kits § 7.411 New technology. MSHA may approve cable products or splice kits that incorporate technology...