Electrical and thermal conductivities of Stycast 1266 epoxy/graphite composites

International Nuclear Information System (INIS)

Tien, Hoang; Park, Joonkyu; Han, Sanga; Ahmad, Muneer; Seo, Yongho; Shin, Koo

2011-01-01

Nanocomposites composed of graphene flakes and epoxy resin (Stycast 1266) were produced with different concentrations of graphene in the range of 0 to 15 wt.%. The direct-current conductivity of the composites complied with percolation behavior. The percolation threshold concentration pc from the conductivity measurement was estimated as 8 wt.%, and the critical exponent as t = 1.85 ± 0.23. The alternating-current conductivity of the composite increased monotonically as the frequency was increased in the range from 1 to 10 MHz. The thermal conductivity k also exhibited a similar percolation behavior, with highest value of k = 0.73 W/m·K for the 12-wt.% composite, which corresponds to a 350% enhancement of the thermal conductivity.

Resource Prospector (RP) - Early Prototyping and Development

Science.gov (United States)

Andrews, D.; Colaprete, A.; Quinn, J.; Bluethmann, B.; Trimble, J.

2015-01-01

exploration of near-Earth asteroids (NEAs) and Mars. In order to reduce risk and explore system designs, the RP project is attempting two-fold approaches to development as it looks towards flight. We continue to explore flight planning, requirements, and interfaces definition by using Engineering Test Units (ETUs), looking towards lunar deployment, while also using fiscal year 2015 to develop, build and test an earth-terrestrial prototype rover and payload system. This terrestrial prototype, called "RP15", is built to both inform the system design, and to be a partnership advocacy tool for this unique mission. RP15 must be affordable within the resource and time constraints of fiscal year 2015, while working to the following Needs, Goals, and Objectives provided by HEOMD/AES: 1. Demonstrate rover mobility in a 1g environment 2. The Surface Segment (prototype rover + payload system) shall represent the flight system concept with as much fidelity as affordable (limited by cost and schedule) - Surface Segment shall be the approximate size/dimension/footprint -Surface Segment shall package all the expected devices (instruments, systems, etc.), even if some facets are mocked-up due to time/cost constraints -Overall Surface Segment fidelity negotiable to make achievable 3. Priority should be given to illustrating mission functionality over support functionality, which exists solely to support mission functionality This paper will provide an overview of RP project developments, including the design and build, capturing the development and initial integrated testing of RP15 in relevant environments.

New technologies applied to radiation protection training: Rp course for technical qualified expert

International Nuclear Information System (INIS)

Llorente Herranz, Cristina; Rodriguez, M.; Marco Arboli, Marisa

2008-01-01

This paper shows the development and results of the first Course for technical qualified expert in radiation protection (RP) using the advantages of Communication and Information Technologies (CITs). This project in modality b-learning has born as a result of the RP specialised course demand, the necessity of working-private life balance and working-training time combination as well as the geographic widespread of those interested. The methodology selected has been b-learning (blended learning), which consists of a mixture of e-learning plus face-to-face learning. The RP course for technical qualified RP expert has been designed for 10 weeks using online learning methodology and 2 days of face-to-face learning in a radioactive facility to take account practical sessions and the final knowledge evaluation. A multidisciplinary team of experts has elaborated the RP programme and the docent material, following national normative. The material consists of: a) Multimedia material with theoretical content to be visualised online; b) Additional material to be un-load or printed; c) Practical exercises within multimedia content; d) Practical online and face-to-face sessions. (author)

Epidemiologic analysis and evaluation of complications in 1266 cases with maxillofacial trauma

Directory of Open Access Journals (Sweden)

Cenk Demirdover

2018-01-01

Full Text Available Introduction: Patients with maxillofacial trauma represent a major group of patients in plastic surgery practice. These traumas are often caused by in-car and noncar road accidents and can result in permanent damages. This study aims at providing an epidemiologic analysis of such cases together with their diagnostic procedures, treatment methods, and postoperative complications. Materials and Methods: A total of 1266 maxillofacial trauma cases that were operated on between 2003 and 2017 were studied, and analyzed for fractured bones, etiology, diagnosis and treatment principles, and complications. Results: The major etiologic factor causing maxillofacial trauma in our study is noncar road accidents (25.5%. Mandible fractures were seen to be the most common type of bone fracture (52.2%. Together with physical examination, computerized tomography and orthopantomography are the diagnostic procedures we effectively use in our clinic. Rigid and semi-rigid fixation with plate and screws are the most frequently used treatment methods, and complication rates are seen to be lower (by 6.6% than those reported in the literature. Conclusion: Careful and meticulous processes, as well as effective follow-up of the patient are required to achieve optimal esthetic and functional results in maxillofacial trauma cases. Such approach can help to reduce the possibility of complications and allow for their early identification, hence early intervention.

TLR4 accessory molecule RP105 (CD180 regulates monocyte-driven arteriogenesis in a murine hind limb ischemia model.

Directory of Open Access Journals (Sweden)

Antonius J N M Bastiaansen

Full Text Available AIMS: We investigated the role of the TLR4-accessory molecule RP105 (CD180 in post-ischemic neovascularization, i.e. arteriogenesis and angiogenesis. TLR4-mediated activation of pro-inflammatory Ly6Chi monocytes is crucial for effective neovascularization. Immunohistochemical analyses revealed that RP105+ monocytes are present in the perivascular space of remodeling collateral arterioles. As RP105 inhibits TLR4 signaling, we hypothesized that RP105 deficiency would lead to an unrestrained TLR4-mediated inflammatory response and hence to enhanced blood flow recovery after ischemia. METHODS AND RESULTS: RP105-/- and wild type (WT mice were subjected to hind limb ischemia and blood flow recovery was followed by Laser Doppler Perfusion Imaging. Surprisingly, we found that blood flow recovery was severely impaired in RP105-/- mice. Immunohistochemistry showed that arteriogenesis was reduced in these mice compared to the WT. However, both in vivo and ex vivo analyses showed that circulatory pro-arteriogenic Ly6Chi monocytes were more readily activated in RP105-/- mice. FACS analyses showed that Ly6Chi monocytes became activated and migrated to the affected muscle tissues in WT mice following induction of hind limb ischemia. Although Ly6Chi monocytes were readily activated in RP105-/- mice, migration into the ischemic tissues was hampered and instead, Ly6Chi monocytes accumulated in their storage compartments, bone marrow and spleen, in RP105-/- mice. CONCLUSIONS: RP105 deficiency results in an unrestrained inflammatory response and monocyte over-activation, most likely due to the lack of TLR4 regulation. Inappropriate, premature systemic activation of pro-inflammatory Ly6Chi monocytes results in reduced infiltration of Ly6Chi monocytes in ischemic tissues and in impaired blood flow recovery.

Rapid versus delayed stimulation of feeding by the endogenously released AgRP neuron mediators GABA, NPY, and AgRP.

Science.gov (United States)

Krashes, Michael J; Shah, Bhavik P; Koda, Shuichi; Lowell, Bradford B

2013-10-01

Agouti-related peptide (AgRP) neurons of the hypothalamus release a fast transmitter (GABA) in addition to neuropeptides (neuropeptide Y [NPY] and Agouti-related peptide [AgRP]). This raises questions as to their respective functions. The acute activation of AgRP neurons robustly promotes food intake, while central injections of AgRP, NPY, or GABA agonist results in the marked escalation of food consumption with temporal variance. Given the orexigenic capability of all three of these neuroactive substances in conjunction with their coexpression in AgRP neurons, we looked to unravel their relative temporal role in driving food intake. After the acute stimulation of AgRP neurons with DREADD technology, we found that either GABA or NPY is required for the rapid stimulation of feeding, and the neuropeptide AgRP, through action on MC4 receptors, is sufficient to induce feeding over a delayed yet prolonged period. These studies help to elucidate the neurochemical mechanisms of AgRP neurons in controlling temporally distinct phases of eating. Copyright © 2013 Elsevier Inc. All rights reserved.

Experience in initial training required for the recognition of the qualified RP expert in Spain

International Nuclear Information System (INIS)

Rodriguez Suarez, M.; Marco Arboli, M.; Menarguez, J.

2003-01-01

An important point of the actions inside the European framework to achieve the harmonisation of the training programmes and recognition was included in the European directive 96/29/Euratom which includes definition and specific tasks of the European Qualified Expert on Radiation Protection (RP). Basic syllabus for training of those experts was developed in the communication 98/C 133/03 concerning BSS applications. Although, in the Spanish education system, the training and recognition requirements of the high level qualified experts on RP was defined since 1977, until 2001, the figure of the Technical Qualified Expert on RP does not appear in the legal framework. In December 2002, a new regulation of the Spanish Regulatory Body, CSN, about qualifications to obtain the recognition of RP Expert in Spain (both high qualified and technical RP experts) was published. Concerning the qualified expert on RP, (RP Officer), responsible of the RP Service, which takes charge of the effective protection and advise radioactive and nuclear facilities in Rp aspects,has to be authorised by the regulatory body. to obtain the RP officer diploma, conceded by the CSN, an initial training of 300 hours and a three-year minimum experience are required (for X-ray installation a 6-month experience is enough). The technical qualified expert on RP is the worker who carried out the tasks in the a RP Service under the supervision of the RP officer. A Technician Qualified Expert on RP does not need an specific accreditation of the Regulatory Body, but an initial RP training and a three-month minimum experience are required and has hold a certificate by the RP officer. Continuous training is also required and as well has to receive a certificate from the RP officer. Since 1977, The Institute for energy Studies has been implementing specific training courses for those professionals who want to obtain the diploma of RP officer (high degree qualified RP expert), conceded by the CSN. Since then

TLR accessory molecule RP105 (CD180 is involved in post-interventional vascular remodeling and soluble RP105 modulates neointima formation.

Directory of Open Access Journals (Sweden)

Jacco C Karper

Full Text Available BACKGROUND: RP105 (CD180 is TLR4 homologue lacking the intracellular TLR4 signaling domain and acts a TLR accessory molecule and physiological inhibitor of TLR4-signaling. The role of RP105 in vascular remodeling, in particular post-interventional remodeling is unknown. METHODS AND RESULTS: TLR4 and RP105 are expressed on vascular smooth muscle cells (VSMC as well as in the media of murine femoral artery segments as detected by qPCR and immunohistochemistry. Furthermore, the response to the TLR4 ligand LPS was stronger in VSMC from RP105(-/- mice resulting in a higher proliferation rate. In RP105(-/- mice femoral artery cuff placement resulted in an increase in neointima formation as compared to WT mice (4982 ± 974 µm(2 vs.1947 ± 278 µm(2,p = 0.0014. Local LPS application augmented neointima formation in both groups, but in RP105(-/- mice this effect was more pronounced (10316±1243 µm(2 vs.4208 ± 555 µm(2,p = 0.0002, suggesting a functional role for RP105. For additional functional studies, the extracellular domain of murine RP105 was expressed with or without its adaptor protein MD1 and purified. SEC-MALSanalysis showed a functional 2∶2 homodimer formation of the RP105-MD1 complex. This protein complex was able to block the TLR4 response in whole blood ex-vivo. In vivo gene transfer of plasmid vectors encoding the extracellular part of RP105 and its adaptor protein MD1 were performed to initiate a stable endogenous soluble protein production. Expression of soluble RP105-MD1 resulted in a significant reduction in neointima formation in hypercholesterolemic mice (2500 ± 573 vs.6581 ± 1894 µm(2,p<0.05, whereas expression of the single factors RP105 or MD1 had no effect. CONCLUSION: RP105 is a potent inhibitor of post-interventional neointima formation.

Neutron Radiography at the RP-10 reactor

International Nuclear Information System (INIS)

Hinostrosa, H.; Ravello, Y.; Cornejo, N.; Mendoza, M.; Montoya, M.

1992-01-01

The facility of neutron radiography at the RP-10 peruvian research reactor is described. The factor of collimation L/D is 149; the Cadmium ratio for the gold in the inspection's area is 4.5, and the thermal neutrons flux on the sample is 3,14 x 10 6 n/cm 2 s (author). 5 refs. 5 fig

A recombination outside the BB deletion refines the location of the X-linked retinitis pigmentosa locus RP3

Energy Technology Data Exchange (ETDEWEB)

Fujita, R.; Bingham, E.; Forsythe, P.; McHenry, C. [Univ. of Michigan, Ann Arbor, MI (United States)] [and others

1996-07-01

Genetic loci for X-linked retinitis pigmentosa (XLRP) have been mapped between Xp11.22 and Xp22.13 (RP2, RP3, RP6, and RP15). The RP3 gene, which is responsible for the predominant form of XLRP in most Caucasian populations, has been localized to Xp21.1 by linkage analysis and the map positions of chromosomal deletions associated with the disease. Previous linkage studies have suggested that RP3 is flanked by the markers DXS1110 (distal) and OTC (proximal). Patient BB was though to have RP because of a lesion at the RP3 locus, in addition to chronic granulomatous disease, Duchenne muscular dystrophy (DMD), mild mental retardation, and the McLeod phenotype. This patient carried a deletion extending {approximately}3 Mb from DMD in Xp21.3 to Xp21.1, with the proximal breakpoint located {approximately}40 kb centromeric to DXS1110. The RP3 gene, therefore, is believed to reside between DXS1110 and the proximal breakpoint of the BB deletion. In order to refine the location of RP3 and to ascertain patients with RP3, we have been analyzing several XLRP families for linkage to Xp markers. Linkage analysis in an American family of 27 individuals demonstrates segregation of XLRP with markers in Xp21.1, consistent with the RP3 subtype. One affected male shows a recombination event proximal to DXS1110. Additional markers within the DXS1110-OTC interval show that the crossover is between two novel polymorphic markers, DXS8349 and M6, both of which are present in BB DNA and lie centromeric to the proximal breakpoint. This recombination places the XLRP mutation in this family outside the BB deletion and redefines the location of RP3. 22 refs., 3 figs., 2 tabs.

Autosomal recessive retinitis pigmentosa with RP1 mutations is associated with myopia.

Science.gov (United States)

Chassine, Thomas; Bocquet, Béatrice; Daien, Vincent; Avila-Fernandez, Almudena; Ayuso, Carmen; Collin, Rob Wj; Corton, Marta; Hejtmancik, J Fielding; van den Born, L Ingeborgh; Klevering, B Jeroen; Riazuddin, S Amer; Sendon, Nathacha; Lacroux, Annie; Meunier, Isabelle; Hamel, Christian P

2015-10-01

To determine the refractive error in patients with autosomal recessive retinitis pigmentosa (arRP) caused by RP1 mutations and to compare it with that of other genetic subtypes of RP. Twenty-six individuals had arRP with RP1 mutations, 25 had autosomal dominant RP (adRP) with RP1 mutation, 8 and 33 had X-linked RP (xlRP) with RP2 and RPGR mutations, respectively, 198 and 93 had Usher syndrome and arRP without RP1 mutations, respectively. The median of the spherical equivalent (SE) and the IQR (Q25-Q75) was determined and multiple comparisons were performed. arRP patients with RP1 mutations had SE median at -4.0 dioptres (D) OD (Ocula Dextra); -3.88 D OS (Ocula Sinistra), whereas arRP patients without RP1 mutations (-0.50 D OD; -0.75 D OS) and Usher syndrome patients (-0.50 D OD; -0.38 D OS) were significantly less myopic (pUsher syndrome and adRP with RP1 mutation had a narrow IQR (-9.06 to -1.13 D), whereas arRP with RP1 mutations and xlRP with RP2 or RPGR mutations had a larger range (-9.06; -1.13 D). arRP patients with RP1 mutations have myopia not different from patients with xlRP with RP2 or RPGR mutations, while RP patients from other genetic subgroups were emmetropic or mildly myopic. We suggest that arRP patients with high myopic refractive error should be preferentially analysed for RP1 mutations. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.

Radiation Protection Section (SC/SL/RP)

CERN Document Server

2006-01-01

We should like to inform you that the Radiation Protection Section (SC/SL/RP) located on the Prévessin site has moved from Building 865 (ground floor) to new premises in Wing A of Building 892 (second floor). Telephone numbers remain the same. SC/SL/RP section

Comparison of Scientific Research Projects of Education Faculties

Science.gov (United States)

Altunay, Esen; Tonbul, Yilmaz

2015-01-01

Many studies indicate that knowledge and knowledge production are the main predictors of social development, welfare and the ability to face the future with confidence. It could be argued that knowledge production is mainly carried out by universities. This study compares 1266 scientific research projects (SRPs) completed by faculties of education…

Autosomal recessive retinitis pigmentosa with RP1 mutations is associated with myopia

NARCIS (Netherlands)

Chassine, T.; Bocquet, B.; Daien, V.; Avila-Fernandez, A.; Ayuso, C.; Collin, R.W.J.; Corton, M.; Hejtmancik, J.F.; Born, L.I. van den; Klevering, B.J.; Riazuddin, S.A.; Sendon, N.; Lacroux, A.; Meunier, I.; Hamel, C.P.

2015-01-01

OBJECTIVE: To determine the refractive error in patients with autosomal recessive retinitis pigmentosa (arRP) caused by RP1 mutations and to compare it with that of other genetic subtypes of RP. METHODS: Twenty-six individuals had arRP with RP1 mutations, 25 had autosomal dominant RP (adRP) with RP1

An unusual dimeric structure and assembly for TLR4 regulator RP105-MD-1

Energy Technology Data Exchange (ETDEWEB)

Yoon, Sung-il; Hong, Minsun; Wilson, Ian A [Scripps

2011-11-16

RP105-MD-1 modulates the TLR4-MD-2-mediated, innate immune response against bacterial lipopolysaccharide (LPS). The crystal structure of the bovine 1:1 RP105-MD-1 complex bound to a putative endogenous lipid at 2.9 Å resolution shares a similar overall architecture to its homolog TLR4-MD-2 but assembles into an unusual 2:2 homodimer that differs from any other known TLR-ligand assembly. The homodimer is assembled in a head-to-head orientation that juxtaposes the N-terminal leucine-rich repeats (LRRs) of the two RP105 chains, rather than the usual tail-to-tail configuration of C-terminal LRRs in ligand-activated TLR dimers, such as TLR1-TRL2, TLR2-TLR6, TLR3-TLR3 and TLR4-TLR4. Another unusual interaction is mediated by an RP105-specific asparagine-linked glycan, which wedges MD-1 into the co-receptor binding concavity on RP105. This unique mode of assembly represents a new paradigm for TLR complexes and suggests a molecular mechanism for regulating LPS responses.

The VIRGO project

International Nuclear Information System (INIS)

Yvert, M.

1991-01-01

The detection of gravitational waves is, nowadays, technologically possible. The Virgo project, proposed by a French-Italian collaboration, is now on the verge of being fully approved and funded. The goal of the Virgo project is, first, to detect gravitational waves. The next will be to perform, in conjunction with other similar detectors, more precise measurements (i.e. the spin of the graviton) and start gravitational wave astrophysical observations. The method is proposed to use a large Michelson interferometer as a detector. The main motivations, characteristics and expected sensitivity of this project are described. (R.P.) 14 refs., 6 figs

Radiation protection aspects of AECL's retube/refurbishment projects

International Nuclear Information System (INIS)

Zhuang, Y.; Boss, C.R.; Pontikakis, N.

2007-01-01

In contrast to the construction of a new nuclear reactor, Retube/Refurbishment of nuclear reactors that have been in operation for many years will involve fabrication of a new core in a radiation environment. Careful planning of the radiation protection (RP) program is crucial to ensure the protection of workers and the environment, and the success of the projects. This paper describes the key RP activities currently underway in AECL's Retube/Refurbishment projects, covering RP during retubing tooling and system designs, retubing work planning, retubing operation, and waste transfer and management. The discussion will focus on RP initiatives from engineering design aspects of the projects. (author)

Nuclear binding around the RP-process waiting points $^{68}$Se and $^{72}$Kr

CERN Multimedia

2002-01-01

Encouraged by the success of mass determinations of nuclei close to the Z=N line performed at ISOLTRAP during the year 2000 and of the recent decay spectroscopy studies on neutron-deficient Kr isotopes (IS351 collaboration), we aim to measure masses and proton separation energies of the bottleneck nuclei defining the flow of the astrophysical rp-process beyond A$\\sim$70. In detail, the program includes mass measurements of the rp-process waiting point nuclei $^{68}$Se and $^{72}$Kr and determination of proton separation energies of the proton-unbound $^{69}$Br and $^{73}$Rb via $\\beta$-decays of $^{69}$Kr and $^{73}$Sr, respectively. The aim of the project is to complete the experimental database for astrophysical network calculations and for the liquid-drop type of mass models typically used in the modelling of the astrophysical rp process in the region. The first beamtime is scheduled for the August 2001 and the aim is to measure the absolute mass of the waiting-point nucleus $^{72}$Kr.

The Effect of Hydrogen Addition on the Combustion Characteristics of RP-3 Kerosene/Air Premixed Flames

Directory of Open Access Journals (Sweden)

Wen Zeng

2017-07-01

Full Text Available Experimental studies have been performed to investigate the effects of hydrogen addition on the combustion characteristics of Chinese No.3 jet fuel (RP-3 kerosene/air premixed flames. Experiments were carried out in a constant volume chamber and the influences of the initial temperatures of 390 and 420 K, initial pressures of 0.1 and 0.3 MPa, equivalence ratios of 0.6–1.6 and hydrogen additions of 0.0–0.5 on the laminar burning velocities, and Markstein numbers of Hydrogen (H2/RP-3/air mixtures were investigated. The results show that the flame front surfaces of RP-3/air mixtures remain smooth throughout the entire flame propagation process at a temperature of 390 K, pressure of 0.3 MPa, equivalence ratio of 1.3 and without hydrogen addition, but when the hydrogen addition increases from 0.0 to 0.5 under the same conditions, flaws and protuberances occur at the flame surfaces. It was also found that with the increase of the equivalence ratio from 0.9 to 1.5, the laminar burning velocities of the mixtures increase at first and then decrease, and the highest laminar burning velocity was measured at an equivalence ratio of 1.2. Meanwhile, with the increase of hydrogen addition, laminar burning velocities of H2/RP-3/air mixtures increase. However, the Markstein numbers of H2/RP-3/air mixtures decrease with the increase of hydrogen addition, which means that the flames of H2/RP-3/air mixtures become unstable with the increase of hydrogen addition.

Extended abstract: Partial row projection methods

Energy Technology Data Exchange (ETDEWEB)

Bramley, R.; Lee, Y. [Indiana Univ., Bloomington, IN (United States)

1996-12-31

Accelerated row projection (RP) algorithms for solving linear systems Ax = b are a class of iterative methods which in theory converge for any nonsingular matrix. RP methods are by definition ones that require finding the orthogonal projection of vectors onto the null space of block rows of the matrix. The Kaczmarz form, considered here because it has a better spectrum for iterative methods, has an iteration matrix that is the product of such projectors. Because straightforward Kaczmarz method converges slowly for practical problems, typically an outer CG acceleration is applied. Definiteness, symmetry, or localization of the eigenvalues, of the coefficient matrix is not required. In spite of this robustness, work has generally been limited to structured systems such as block tridiagonal matrices because unlike many iterative solvers, RP methods cannot be implemented by simply supplying a matrix-vector multiplication routine. Finding the orthogonal projection of vectors onto the null space of block rows of the matrix in practice requires accessing the actual entries in the matrix. This report introduces a new partial RP algorithm which retains advantages of the RP methods.

School Psychologists in Support of Transgender and Gender Diverse Students in Light of California's AB 1266 (School Success and Opportunity Act): Implications and Opportunities

Science.gov (United States)

Agee-Aguayo, Joseph; Bloomquist, Erik; Savage, Todd A.; Woitaszewski, Scott A.

2017-01-01

The present study examined the attitudes of California-based school psychologists toward transgender-identifying students and assessed their efforts and roles in supporting this student population in light of recently passed legislation in California (AB 1266; 2013), which requires public schools in the state to provide transgender students with…

Arcuate AgRP neurons mediate orexigenic and glucoregulatory actions of ghrelin★

Science.gov (United States)

Wang, Qian; Liu, Chen; Uchida, Aki; Chuang, Jen-Chieh; Walker, Angela; Liu, Tiemin; Osborne-Lawrence, Sherri; Mason, Brittany L.; Mosher, Christina; Berglund, Eric D.; Elmquist, Joel K.; Zigman, Jeffrey M.

2013-01-01

The hormone ghrelin stimulates eating and helps maintain blood glucose upon caloric restriction. While previous studies have demonstrated that hypothalamic arcuate AgRP neurons are targets of ghrelin, the overall relevance of ghrelin signaling within intact AgRP neurons is unclear. Here, we tested the functional significance of ghrelin action on AgRP neurons using a new, tamoxifen-inducible AgRP-CreERT2 transgenic mouse model that allows spatiotemporally-controlled re-expression of physiological levels of ghrelin receptors (GHSRs) specifically in AgRP neurons of adult GHSR-null mice that otherwise lack GHSR expression. AgRP neuron-selective GHSR re-expression partially restored the orexigenic response to administered ghrelin and fully restored the lowered blood glucose levels observed upon caloric restriction. The normalizing glucoregulatory effect of AgRP neuron-selective GHSR expression was linked to glucagon rises and hepatic gluconeogenesis induction. Thus, our data indicate that GHSR-containing AgRP neurons are not solely responsible for ghrelin's orexigenic effects but are sufficient to mediate ghrelin's effects on glycemia. PMID:24567905

Arcuate AgRP neurons mediate orexigenic and glucoregulatory actions of ghrelin.

Science.gov (United States)

Wang, Qian; Liu, Chen; Uchida, Aki; Chuang, Jen-Chieh; Walker, Angela; Liu, Tiemin; Osborne-Lawrence, Sherri; Mason, Brittany L; Mosher, Christina; Berglund, Eric D; Elmquist, Joel K; Zigman, Jeffrey M

2014-02-01

The hormone ghrelin stimulates eating and helps maintain blood glucose upon caloric restriction. While previous studies have demonstrated that hypothalamic arcuate AgRP neurons are targets of ghrelin, the overall relevance of ghrelin signaling within intact AgRP neurons is unclear. Here, we tested the functional significance of ghrelin action on AgRP neurons using a new, tamoxifen-inducible AgRP-CreER(T2) transgenic mouse model that allows spatiotemporally-controlled re-expression of physiological levels of ghrelin receptors (GHSRs) specifically in AgRP neurons of adult GHSR-null mice that otherwise lack GHSR expression. AgRP neuron-selective GHSR re-expression partially restored the orexigenic response to administered ghrelin and fully restored the lowered blood glucose levels observed upon caloric restriction. The normalizing glucoregulatory effect of AgRP neuron-selective GHSR expression was linked to glucagon rises and hepatic gluconeogenesis induction. Thus, our data indicate that GHSR-containing AgRP neurons are not solely responsible for ghrelin's orexigenic effects but are sufficient to mediate ghrelin's effects on glycemia.

Short vowel placements in RP past and present

DEFF Research Database (Denmark)

Fabricius, Anne

This study addresses diachronic change in the short vowel system of RP. While TRAP lowering and backing in RP has been reported previously, the movements STRUT has undergone have proven more difficult to determine. This study identifies a TRAP/STRUT 'rotation' using acoustic measurements...

Swirl Coaxial Injector Testing with LOX/RP-J

Science.gov (United States)

Greene, Sandra Elam; Casiano, Matt

2013-01-01

Testing was conducted at NASA fs Marshall Space Flight Center (MSFC) in the fall of 2012 to evaluate the operation and performance of liquid oxygen (LOX) and kerosene (RP ]1) in an existing swirl coaxial injector. While selected Russian engines use variations of swirl coaxial injectors, component level performance data has not been readily available, and all previously documented component testing at MSFC with LOX/RP ]1 had been performed using a variety of impinging injector designs. Impinging injectors have been adequate for specific LOX/RP ]1 engine applications, yet swirl coaxial injectors offer easier fabrication efforts, providing cost and schedule savings for hardware development. Swirl coaxial elements also offer more flexibility for design changes. Furthermore, testing with LOX and liquid methane propellants at MSFC showed that a swirl coaxial injector offered improved performance compared to an impinging injector. So, technical interest was generated to see if similar performance gains could be achieved with LOX/RP ]1 using a swirl coaxial injector. Results would allow such injectors to be considered for future engine concepts that require LOX/RP ]1 propellants. Existing injector and chamber hardware was used in the test assemblies. The injector had been tested in previous programs at MSFC using LOX/methane and LOX/hydrogen propellants. Minor modifications were made to the injector to accommodate the required LOX/RP ]1 flows. Mainstage tests were performed over a range of chamber pressures and mixture ratios. Additional testing included detonated gbombs h for stability data. Test results suggested characteristic velocity, C*, efficiencies for the injector were 95 ]97%. The injector also appeared dynamically stable with quick recovery from the pressure perturbations generated in the bomb tests.

Arcuate AgRP neurons mediate orexigenic and glucoregulatory actions of ghrelin

OpenAIRE

Wang, Qian; Liu, Chen; Uchida, Aki; Chuang, Jen-Chieh; Walker, Angela; Liu, Tiemin; Osborne-Lawrence, Sherri; Mason, Brittany L.; Mosher, Christina; Berglund, Eric D.; Elmquist, Joel K.; Zigman, Jeffrey M.

2014-01-01

The hormone ghrelin stimulates eating and helps maintain blood glucose upon caloric restriction. While previous studies have demonstrated that hypothalamic arcuate AgRP neurons are targets of ghrelin, the overall relevance of ghrelin signaling within intact AgRP neurons is unclear. Here, we tested the functional significance of ghrelin action on AgRP neurons using a new, tamoxifen-inducible AgRP-CreERT2 transgenic mouse model that allows spatiotemporally-controlled re-expression of physiologi...

RP-2 Thermal Stability and Heat Transfer Investigation for Hydrocarbon Boost Engines

Science.gov (United States)

VanNoord, J. L.; Stiegemeier, B. R.

2010-01-01

A series of electrically heated tube tests were performed at the NASA Glenn Research Center s Heated Tube Facility to investigate the use of RP-2 as a fuel for next generation regeneratively cooled hydrocarbon boost engines. The effect that test duration, operating condition and test piece material have on the overall thermal stability and materials compatibility characteristics of RP-2 were evaluated using copper and 304 stainless steel test sections. The copper tests were run at 1000 psia, heat flux up to 6.0 Btu/in.2-sec, and wall temperatures up to 1180 F. Preliminary results, using measured wall temperature as an indirect indicator of the carbon deposition process, show that in copper test pieces above approximately 850 F, RP-2 begins to undergo thermal decomposition resulting in local carbon deposits. Wall temperature traces show significant local temperature increases followed by near instantaneous drops which have been attributed to the carbon deposition/shedding process in previous investigations. Data reduction is currently underway for the stainless steel test sections and carbon deposition measurements will be performed in the future for all test sections used in this investigation. In conjunction with the existing thermal stability database, these findings give insight into the feasibility of cooling a long life, high performance, high-pressure liquid rocket combustor and nozzle with RP-2.

On-the-job training as new element in RP training: experiences from the ENETRAP pilot module in Karlsruhe

International Nuclear Information System (INIS)

Moebius, Siegurd; Bickel, Angela; Schmitt-Hannig, Annemarie; Coeck, Michele

2008-01-01

A suitable qualification for responsible personnel in Radiation Protection (RP) must be in general a combination of theoretical knowledge, and the ability (competency) to practice RP. While the theoretical knowledge is acquired by suitable education and by attending training courses, competency and skills can only be obtained by appropriate on-the-job training (OJT) followed by a period of work experience. From the feedback of questionnaires from 30 EU countries within the framework of the EU supported ENETRAP project it can be concluded that OJT provides better chances for future job opportunities and increases international flexibility. As result we recommend covering OJT together with education and training in the Basic Safety Standards and their guidelines for implementation. OJT should be specified by its content (syllabus, learning objectives), availability of necessary facilities and infrastructures as precondition for OJT, assessment of the competence of the participant, format of certificate, recognition of OJT, and responsibilities of host organisation and trainees. OJT should remain a key element in the remodelled 'European RP Training'. Based on these recommendations a two weeks training module on 'Occupational RP: Specificities of Waste Management and Decommissioning' designed for radiation protection professionals has been compiled at the Karlsruhe Training Centre FTU. While the first week of the pilot course focuses on the theoretical knowledge ranging from waste classification to decontamination techniques and transport of radioactive materials, the second week is addressed to practical training as OJT from clearance of radioactive waste, operative RP in the Decontamination Department to RP work in a Research Reactor under decommissioning. Special emphasis is devoted to RP aspects and active involvement of the participants in workshops and case studies. The results of the pilot run with participants from 8 different European countries are reported

30 CFR 881.6 - Project contract.

Science.gov (United States)

2010-07-01

... contractors or suppliers for the construction, installation, services or work to be performed. (b) Project... 30 Mineral Resources 3 2010-07-01 2010-07-01 false Project contract. 881.6 Section 881.6 Mineral... LAND RECLAMATION SUBSIDENCE AND STRIP MINE REHABILITATION, APPALACHIA § 881.6 Project contract. (a...

7 CFR 2903.6 - Project types.

Science.gov (United States)

2010-01-01

... 7 Agriculture 15 2010-01-01 2010-01-01 false Project types. 2903.6 Section 2903.6 Agriculture... AGRICULTURE BIODIESEL FUEL EDUCATION PROGRAM Program Description § 2903.6 Project types. OEPNU intends to... predetermined project period with a statement of intention to provide additional support at a future date...

RpA ratio: total shadowing due to running coupling

OpenAIRE

Iancu, E.; Triantafyllopoulos, D. N.

2007-01-01

We predict that the RpA ratio at the most forward rapidities to be measured at LHC should be strongly suppressed, close to "total shadowing'' (RpA = A^(-1/3)), as a consequence of running coupling effects in the nonlinear QCD evolution.

6 Sigma project advance

International Nuclear Information System (INIS)

2002-12-01

This book deals with 6 sigma project advance which introduces 6 sigma project in Changwon special steel, how is failure accepted? CTQ selection which is starting line, definition of performance standard, measurement system check on reliability of measurement data, check of process capacity for current level, establishment of target, optimal design and performance of application, practice of management system for maintain of improved result, CTQ selection, check of measurement system and practice of management system.

New Inhibitors of the DENV-NS5 RdRp from Carpolepis laurifolia as Potential Antiviral Drugs for Dengue Treatment

Directory of Open Access Journals (Sweden)

Paul Coulerie

2014-05-01

Full Text Available Since a few decades the dengue virus became a major public health concern and no treatment is available yet. In order to propose potential antidengue compounds for chemotherapy we focused on DENV RNA polymerase (DENV-NS5 RdRp which is specific and essential for the virus replication. Carpolepis laurifolia belongs to the Myrtaceae and is used as febrifuge in traditional kanak medicine. Leaf extract of this plant has been identified as a hit against the DENV-NS5 RdRp. Here we present a bioguided fractionation of the leaf extract of C. laurifolia which is also the first phytochemical evaluation of this plant. Five flavonoids, namely quercetin (1, 6-methyl-7-methoxyapigenin (2, avicularin (3, quercitrin (4 and hyperoside (5, together with betulinic acid (6, were isolated from the leaf extract of C. laurifolia. All isolated compounds were tested individually against the DENV-NS5 RdRp and compared with four other commercial flavonoids: isoquercitrin (7, spiraeoside (8, quercetin-3,4’-di-O-glucoside (9 and rutine (10. Compounds 3, 4, 6, 8 and 10 displayed IC 50 ranging from 1.7 to 2.1 µM, and were the most active against the DENV-NS5 RdRp.

ACADEMIC TRAINING (R.P. Walker)

CERN Multimedia

Françoise Benz

2002-01-01

15, 16, 17 May LECTURE SERIES from 11.00 to 12.00 hrs - Council room, bldg. 503 on 15 May, Auditorium, bldg. 500 on 16 and 17 May Introduction to free electron lasers by R.P. Walker / Rutherford Laboratory, UK The Free-electron laser (FEL) is a source of coherent electromagnetic radiation based on a relativistic electron beam. First operated 25 years ago, the FEL has now reached a stage of maturity for operation in the infra-red region of the spectrum and several facilities provide intense FEL radiation beams for research covering a wide range of disciplines. Several projects both underway and proposed aim at pushing the minimum wavelength from its present limit around 100 nm progressively down to the 1 Angstrom region where the X-ray FEL would open up many new and exciting research possibilities. Other developments aim at increasing power levels to the 10's of kW level. In this series of lectures we give an introduction to the basic principles of FELs and their different modes of operation, and summarise the...

Exploring Phonetic and Phonological Variation: RP and the Nigerian ...

African Journals Online (AJOL)

... Nigerian university, a hierarchy of the intelligibility of RP vowel phonemes is established. This not only provides evidence that intelligibility is a phenomenon which may be examined from a non-native speaker perspective, it also identifies specific features of RP segmental phonology which presents problems to Nigerians.

High-precision mass measurements for the rp-process at JYFLTRAP

Directory of Open Access Journals (Sweden)

Canete Laetitia

2017-01-01

Full Text Available The double Penning trap JYFLTRAP at the University of Jyväskylä has been successfully used to achieve high-precision mass measurements of nuclei involved in the rapid proton-capture (rp process. A precise mass measurement of 31Cl is essential to estimate the waiting point condition of 30S in the rp-process occurring in type I x-ray bursts (XRBs. The mass-excess of 31C1 measured at JYFLTRAP, -7034.7(3.4 keV, is 15 more precise than the value given in the Atomic Mass Evaluation 2012. The proton separation energy Sp determined from the new mass-excess value confirmed that 30S is a waiting point, with a lower-temperature limit of 0.44 GK. The mass of 52Co effects both 51Fe(p,γ52Co and 52Co(p,γ53Ni reactions. The mass-excess value measured, - 34 331.6(6.6 keV is 30 times more precise than the value given in AME2012. The Q values for the 51Fe(p,γ52Co and 52Co(p,γ53Ni reactions are now known with a high precision, 1418(11 keV and 2588(26 keV respectively. The results show that 52Co is more proton bound and 53Ni less proton bound than what was expected from the extrapolated value.

Calibration of "Babyline" RP instruments

CERN Multimedia

2015-01-01

  If you have old RP instrumentation of the “Babyline” type, as shown in the photo, please contact the Radiation Protection Group (Joffrey Germa, 73171) to have the instrument checked and calibrated. Thank you. Radiation Protection Group

Measurement of the Diffusion Coefficient of Water in RP-3 and RP-5 Jet Fuels Using Digital Holography Interferometry

Science.gov (United States)

Li, Chaoyue; Feng, Shiyu; Shao, Lei; Pan, Jun; Liu, Weihua

2018-04-01

The diffusion coefficient of water in jet fuel was measured employing double-exposure digital holographic interferometry to clarify the diffusion process and make the aircraft fuel system safe. The experimental method and apparatus are introduced in detail, and the digital image processing program is coded in MATLAB according to the theory of the Fourier transform. At temperatures ranging from 278.15 K to 333.15 K in intervals of 5 K, the diffusion coefficient of water in RP-3 and RP-5 jet fuels ranges from 2.6967 × 10 -10 m2·s-1 to 8.7332 × 10 -10 m2·s-1 and from 2.3517 × 10 -10 m2·s-1 to 8.0099 × 10-10 m2·s-1, respectively. The relationship between the measured diffusion coefficient and temperature can be well fitted by the Arrhenius law. The diffusion coefficient of water in RP-3 jet fuel is higher than that of water in RP-5 jet fuel at the same temperature. Furthermore, the viscosities of the two jet fuels were measured and found to be expressible in the form of the Arrhenius equation. The relationship among the diffusion coefficient, viscosity and temperature is analyzed according to the classic prediction model, namely the Stokes-Einstein correlation, and this correlation is further revised via experimental data to obtain a more accurate predication result.

RP-HPLC/MS-APCI Analysis of Branched Chain TAG Prepared by Precursor-Directed Biosynthesis with Rhodococcus erythropolis

Czech Academy of Sciences Publication Activity Database

Schreiberová, O.; Krulikovská, T.; Sigler, Karel; Čejková, A.; Řezanka, Tomáš

2010-01-01

Roč. 45, č. 8 (2010), s. 743-756 ISSN 0024-4201 R&D Projects: GA MŠk 2B08062 Institutional research plan: CEZ:AV0Z50200510 Keywords : Rhodococcus erythropolis * RP-HPLC/MS-APCI * Branched chain triacylglycerols Subject RIV: EE - Microbiology, Virology Impact factor: 2.151, year: 2010

Hypothalamic AgRP-neurons control peripheral substrate utilization and nutrient partitioning

Science.gov (United States)

Joly-Amado, Aurélie; Denis, Raphaël G P; Castel, Julien; Lacombe, Amélie; Cansell, Céline; Rouch, Claude; Kassis, Nadim; Dairou, Julien; Cani, Patrice D; Ventura-Clapier, Renée; Prola, Alexandre; Flamment, Melissa; Foufelle, Fabienne; Magnan, Christophe; Luquet, Serge

2012-01-01

Obesity-related diseases such as diabetes and dyslipidemia result from metabolic alterations including the defective conversion, storage and utilization of nutrients, but the central mechanisms that regulate this process of nutrient partitioning remain elusive. As positive regulators of feeding behaviour, agouti-related protein (AgRP) producing neurons are indispensible for the hypothalamic integration of energy balance. Here, we demonstrate a role for AgRP-neurons in the control of nutrient partitioning. We report that ablation of AgRP-neurons leads to a change in autonomic output onto liver, muscle and pancreas affecting the relative balance between lipids and carbohydrates metabolism. As a consequence, mice lacking AgRP-neurons become obese and hyperinsulinemic on regular chow but display reduced body weight gain and paradoxical improvement in glucose tolerance on high-fat diet. These results provide a direct demonstration of a role for AgRP-neurons in the coordination of efferent organ activity and nutrient partitioning, providing a mechanistic link between obesity and obesity-related disorders. PMID:22990237

Rp58 and p27kip1 coordinate cell cycle exit and neuronal migration within the embryonic mouse cerebral cortex.

Science.gov (United States)

Clément, Olivier; Hemming, Isabel Anne; Gladwyn-Ng, Ivan Enghian; Qu, Zhengdong; Li, Shan Shan; Piper, Michael; Heng, Julian Ik-Tsen

2017-05-15

During the development of the mammalian cerebral cortex, newborn postmitotic projection neurons are born from local neural stem cells and must undergo radial migration so as to position themselves appropriately to form functional neural circuits. The zinc finger transcriptional repressor Rp58 (also known as Znf238 or Zbtb18) is critical for coordinating corticogenesis, but its underlying molecular mechanism remains to be better characterised. Here, we demonstrate that the co-expression of Rp58 and the cyclin dependent kinase inhibitor (CDKI) p27 kip1 is important for E14.5-born cortical neurons to coordinate cell cycle exit and initiate their radial migration. Notably, we find that the impaired radial positioning of Rp58-deficient cortical neurons within the embryonic (E17.5) mouse cortex, as well as their multipolar to bipolar transition from the intermediate zone to the cortical plate can be restored by forced expression of p27 kip1 in concert with suppression of Rnd2, a downstream target gene of Rp58. Furthermore, the restorative effects of p27 kip1 and Rnd2 abrogation are reminiscent of suppressing RhoA signalling in Rp58-deficient cells. Our findings demonstrate functional interplay between a transcriptional regulator and a CDKI to mediate neuroprogenitor cell cycle exit, as well as to promote radial migration through a molecular mechanism consistent with suppression of RhoA signalling.

Clinical study with a new radiopharmaceutical (RP-517) for the in vivo labeling of neutrophils with 99mTc

International Nuclear Information System (INIS)

Parysow, O.; Mele, A.; Sarmiento, A.; Zubillaga, M.; Caro, R.; Calmanovici, G.; Nicolini, J.; Lazewatsky, J.; Camin, L.

2002-01-01

RP-517 is a 99m Tc labeled antagonist to the LTB 4 receptor which, with a single i.v. injection, allows the in vivo labeling of neutrophils for the detection and localization of infectious and/or inflammatory foci. Aim: To determine: 1) the safety and tolerance of the radiopharmaceutical in humans; 2) its pharmacokinetics and dosimetry; 3) its preliminary efficacy for the detection and localization of infectious foci. Materials and Methods: 20 subjects (14M, 6F) received 592 ± 111 MBq (16 ± 3 mCi) of RP-517 intravenously. All subjects were evaluated (medical history, physical exam, vital signs, ECG, blood and urine analysis) before and at several times up to 24 hours post-injection of RP-517. Blood samples were taken to determine pharmacokinetics and blood clearance. Urine and feces were collected to measure excretion. For dosimetry determination, whole body images, one transmission pre-injection and sequential emission images post-injection, were obtained with a dual head gamma camera (ADAC) in 2 patients and 14 volunteers. Sequential focal images were obtained in 4 patients. The presence of infection was determined by culture and/or follow-up. Results: There were no adverse clinical events or clinically significant changes in any of the laboratory analysis parameters, ECG or vital signs. RP-517 clears the blood mainly through the hepatobiliary system. The critical organ is the gall bladder wall, followed by the intestinal wall. A suggested maximum injected dose of about 740MBq of RP-517 would deliver ≤ 50 mGy to the critical organ. There were 6 patients with suspicion of infection: in 2 of them the infection was proven; a third subject had 2 suspected infectious foci, one was proven, the other one was equivocal; at the time of imaging, there was no infections process in the other 3 subjects. RP-517 images showed a clear and persistent uptake in the 3 proven infectious foci, was negative in the equivocal one and in the three patients without infection

A UKSeRP for SAIL: striking a balance

Directory of Open Access Journals (Sweden)

Kerina Jones

2017-04-01

The SAIL UKSeRP represents a powerful analytical environment within a privacy-protecting safe haven and secure remote access system which has been designed to be scalable and adaptable to meet the needs of the rapidly growing data linkage community. Further challenges lie ahead as the landscape develops and emerging data types become more available. UKSeRP technology is available and customisable for other use cases within the UK and international jurisdictions, to operate within their respective governance frameworks.

AgRP Neurons Can Increase Food Intake during Conditions of Appetite Suppression and Inhibit Anorexigenic Parabrachial Neurons.

Science.gov (United States)

Essner, Rachel A; Smith, Alison G; Jamnik, Adam A; Ryba, Anna R; Trutner, Zoe D; Carter, Matthew E

2017-09-06

To maintain energy homeostasis, orexigenic (appetite-inducing) and anorexigenic (appetite suppressing) brain systems functionally interact to regulate food intake. Within the hypothalamus, neurons that express agouti-related protein (AgRP) sense orexigenic factors and orchestrate an increase in food-seeking behavior. In contrast, calcitonin gene-related peptide (CGRP)-expressing neurons in the parabrachial nucleus (PBN) suppress feeding. PBN CGRP neurons become active in response to anorexigenic hormones released following a meal, including amylin, secreted by the pancreas, and cholecystokinin (CCK), secreted by the small intestine. Additionally, exogenous compounds, such as lithium chloride (LiCl), a salt that creates gastric discomfort, and lipopolysaccharide (LPS), a bacterial cell wall component that induces inflammation, exert appetite-suppressing effects and activate PBN CGRP neurons. The effects of increasing the homeostatic drive to eat on feeding behavior during appetite suppressing conditions are unknown. Here, we show in mice that food deprivation or optogenetic activation of AgRP neurons induces feeding to overcome the appetite suppressing effects of amylin, CCK, and LiCl, but not LPS. AgRP neuron photostimulation can also increase feeding during chemogenetic-mediated stimulation of PBN CGRP neurons. AgRP neuron stimulation reduces Fos expression in PBN CGRP neurons across all conditions. Finally, stimulation of projections from AgRP neurons to the PBN increases feeding following administration of amylin, CCK, and LiCl, but not LPS. These results demonstrate that AgRP neurons are sufficient to increase feeding during noninflammatory-based appetite suppression and to decrease activity in anorexigenic PBN CGRP neurons, thereby increasing food intake during homeostatic need. SIGNIFICANCE STATEMENT The motivation to eat depends on the relative balance of activity in distinct brain regions that induce or suppress appetite. An abnormal amount of activity in

RP-HPLC Determination of vitamins B1, B3, B6, folic acid and B12 in multivitamin tablets

Directory of Open Access Journals (Sweden)

SOTE VLADIMIROV

2005-10-01

Full Text Available Abstract:Asimple and sensitive reversed-phase, ion-pair HPLC method was developed and validated for the simultaneous determination of B-group vitamins, thiamine chloride hydrochloride (B1, nicotinamide (B3, pyridoxine hydrochloride (B6 and folic acid in Pentovit® coated tablets. The cyanocobalamine (B12 was determined separately, because of its low concentration in the investigated multivitamin preparation. RP-HPLC analysis was performed with a LKB 2150 HPLC system, equipped with a UV/VIS Waters M484 detector. The procedures for the determination of B1, B2, B6 and folic acid were carried out on a Supelcosil ABZ+ (15 cm 4.6 mm; 5 µm column with methanol-5mM heptanesulphonic acid sodium salt 0.1%triethylamine TEA(25:75 V/V; pH 2.8 as themobile phase. For the determination of B12 a Suplex pKb-100 (15 cm 4.6 mm; 5 µm column andmethanol–water (22:78 V/V as themobile phase were used. The column effluentsweremonitored at 290 nm for B 1, B3, B6 and folic acid, and at 550 nm for B12. The obtained results and statistical parameters for all the investigated vitamins of the B-group in Pentovit® coated tablets were satisfactory and ranged from 90.4 % to 108.5 % (RSD. from 0.5% to 4.1 %. The parameters for the validation of the methods are given.

Temperature Jump Pyrolysis Studies of RP 2 Fuel

Science.gov (United States)

2017-01-09

Briefing Charts 3. DATES COVERED (From - To) 15 December 2016 – 11 January 2017 4. TITLE AND SUBTITLE Temperature Jump Pyrolysis Studies of RP-2 Fuel...Rev. 8- 98) Prescribed by ANSI Std. 239.18 1 TEMPERATURE JUMP PYROLYSIS STUDIES OF RP-2 FUEL Owen Pryor1, Steven D. Chambreau2, Ghanshyam L...17026 7 Temperature Jump Pyrolysis at AFRL Edwards Rapid heating of a metal filament at a rate of 600 – 800 K/s, and the set temperature is held for

RP1 is a phosphorylation target of CK2 and is involved in cell adhesion.

Science.gov (United States)

Stenner, Frank; Liewen, Heike; Göttig, Stephan; Henschler, Reinhard; Markuly, Norbert; Kleber, Sascha; Faust, Michael; Mischo, Axel; Bauer, Stefan; Zweifel, Martin; Knuth, Alexander; Renner, Christoph; Wadle, Andreas

2013-01-01

RP1 (synonym: MAPRE2, EB2) is a member of the microtubule binding EB1 protein family, which interacts with APC, a key regulatory molecule in the Wnt signalling pathway. While the other EB1 proteins are well characterized the cellular function and regulation of RP1 remain speculative to date. However, recently RP1 has been implicated in pancreatic cancerogenesis. CK2 is a pleiotropic kinase involved in adhesion, proliferation and anti-apoptosis. Overexpression of protein kinase CK2 is a hallmark of many cancers and supports the malignant phenotype of tumor cells. In this study we investigate the interaction of protein kinase CK2 with RP1 and demonstrate that CK2 phosphorylates RP1 at Ser(236) in vitro. Stable RP1 expression in cell lines leads to a significant cleavage and down-regulation of N-cadherin and impaired adhesion. Cells expressing a Phospho-mimicking point mutant RP1-ASP(236) show a marked decrease of adhesion to endothelial cells under shear stress. Inversely, we found that the cells under shear stress downregulate endogenous RP1, most likely to improve cellular adhesion. Accordingly, when RP1 expression is suppressed by shRNA, cells lacking RP1 display significantly increased cell adherence to surfaces. In summary, RP1 phosphorylation at Ser(236) by CK2 seems to play a significant role in cell adhesion and might initiate new insights in the CK2 and EB1 family protein association.

RP1 is a phosphorylation target of CK2 and is involved in cell adhesion.

Directory of Open Access Journals (Sweden)

Frank Stenner

Full Text Available RP1 (synonym: MAPRE2, EB2 is a member of the microtubule binding EB1 protein family, which interacts with APC, a key regulatory molecule in the Wnt signalling pathway. While the other EB1 proteins are well characterized the cellular function and regulation of RP1 remain speculative to date. However, recently RP1 has been implicated in pancreatic cancerogenesis. CK2 is a pleiotropic kinase involved in adhesion, proliferation and anti-apoptosis. Overexpression of protein kinase CK2 is a hallmark of many cancers and supports the malignant phenotype of tumor cells. In this study we investigate the interaction of protein kinase CK2 with RP1 and demonstrate that CK2 phosphorylates RP1 at Ser(236 in vitro. Stable RP1 expression in cell lines leads to a significant cleavage and down-regulation of N-cadherin and impaired adhesion. Cells expressing a Phospho-mimicking point mutant RP1-ASP(236 show a marked decrease of adhesion to endothelial cells under shear stress. Inversely, we found that the cells under shear stress downregulate endogenous RP1, most likely to improve cellular adhesion. Accordingly, when RP1 expression is suppressed by shRNA, cells lacking RP1 display significantly increased cell adherence to surfaces. In summary, RP1 phosphorylation at Ser(236 by CK2 seems to play a significant role in cell adhesion and might initiate new insights in the CK2 and EB1 family protein association.

Nutritive, Post-ingestive Signals Are the Primary Regulators of AgRP Neuron Activity

Directory of Open Access Journals (Sweden)

Zhenwei Su

2017-12-01

Full Text Available Summary: The brain regulates food intake by processing sensory cues and peripheral physiological signals, but the neural basis of this integration remains unclear. Hypothalamic, agouti-related protein (AgRP-expressing neurons are critical regulators of food intake. AgRP neuron activity is high during hunger and is rapidly reduced by the sight and smell of food. Here, we reveal two distinct components of AgRP neuron activity regulation: a rapid but transient sensory-driven signal and a slower, sustained calorie-dependent signal. We discovered that nutrients are necessary and sufficient for sustained reductions in AgRP neuron activity and that activity reductions are proportional to the calories obtained. This change in activity is recapitulated by exogenous administration of gut-derived satiation signals. Furthermore, we showed that the nutritive value of food trains sensory systems—in a single trial—to drive rapid, anticipatory AgRP neuron activity inhibition. Together, these data demonstrate that nutrients are the primary regulators of AgRP neuron activity. : Su et al. demonstrate that nutrient content in the GI tract is rapidly signaled to hypothalamic neurons activated by hunger. This rapid effect is mediated by three satiation signals that synergistically reduce the activity of AgRP neurons. These findings uncover how hunger circuits in the brain are regulated and raise the possibility that hunger can be pharmacologically controlled. Keywords: calcium imaging, AgRP neurons, calories, satiation signals, sensory regulation, single trial learning, cholecystokinin, CCK, peptide tyrosine tyrosine, PYY, amylin, homeostasis

Fabricating Complete Dentures with CAD/CAM and RP Technologies.

Science.gov (United States)

Bilgin, Mehmet Selim; Erdem, Ali; Aglarci, Osman Sami; Dilber, Erhan

2015-06-01

Two techological approaches for fabricating dentures; computer-aided design and computer-aided manufacturing (CAD/CAM) and rapid prototyping (RP), are combined with the conventional techniques of impression and jaw relation recording to determine their feasibility and applicability. Maxillary and mandibular edentulous jaw models were produced using silicone molds. After obtaining a gypsum working model, acrylic bases were crafted, and occlusal rims for each model were fabricated with previously determined standard vertical and centric relationships. The maxillary and mandibular relationships were recorded with guides. The occlusal rims were then scanned with a digital scanner. The alignment of the maxillary and mandibular teeth was verified. The teeth in each arch were fabricated in one piece, or set, either by CAM or RP. Conventional waxing and flasking was then performed for both methods. These techniques obviate a practitioner's need for technicians during design and provide the patient with an opportunity to participate in esthetic design with the dentist. In addition, CAD/CAM and RP reduce chair time; however, the materials and techniques need further improvements. Both CAD/CAM and RP techniques seem promising for reducing chair time and allowing the patient to participate in esthetics design. Furthermore, the one-set aligned artificial tooth design may increase the acrylic's durability. © 2015 by the American College of Prosthodontists.

RP58 Regulates the Multipolar-Bipolar Transition of Newborn Neurons in the Developing Cerebral Cortex

Directory of Open Access Journals (Sweden)

Chiaki Ohtaka-Maruyama

2013-02-01

Full Text Available Accumulating evidence suggests that many brain diseases are associated with defects in neuronal migration, suggesting that this step of neurogenesis is critical for brain organization. However, the molecular mechanisms underlying neuronal migration remain largely unknown. Here, we identified the zinc-finger transcriptional repressor RP58 as a key regulator of neuronal migration via multipolar-to-bipolar transition. RP58−/− neurons exhibited severe defects in the formation of leading processes and never shifted to the locomotion mode. Cre-mediated deletion of RP58 using in utero electroporation in RP58flox/flox mice revealed that RP58 functions in cell-autonomous multipolar-to-bipolar transition, independent of cell-cycle exit. Finally, we found that RP58 represses Ngn2 transcription to regulate the Ngn2-Rnd2 pathway; Ngn2 knockdown rescued migration defects of the RP58−/− neurons. Our findings highlight the critical role of RP58 in multipolar-to-bipolar transition via suppression of the Ngn2-Rnd2 pathway in the developing cerebral cortex.

RP-HPLCdetermination of ginsenosides RG1 and RB1 in Panax ...

African Journals Online (AJOL)

Y Liu, Q He, D Liu, W Wan, X Liu, C Wen, W Fang, W Zhou, Z Xia ... RP-HPLC method is adopted, column used is a ZOBAX SB-C18 column (4.6 mm × 250 mnl, 5 μm), mobile phase A is water, and B is acetonitrile, gradient elution conditions are: 0~20 min (A:B 20:80), 20~60 min (A:B 20~35: 80~65); and detection ...

AgRP neurons regulate development of dopamine neuronal plasticity and nonfood-associated behaviors

Science.gov (United States)

Dietrich, Marcelo O; Bober, Jeremy; Ferreira, Jozélia G; Tellez, Luis A; Mineur, Yann S; Souza, Diogo O; Gao, Xiao-Bing; Picciotto, Marina R; Araújo, Ivan; Liu, Zhong-Wu; Horvath, Tamas L

2012-01-01

It is not known whether behaviors unrelated to feeding are affected by hypothalamic regulators of hunger. We found that impairment of Agouti-related protein (AgRP) circuitry by either Sirt1 knockdown in AgRP-expressing neurons or early postnatal ablation of these neurons increased exploratory behavior and enhanced responses to cocaine. In AgRP circuit–impaired mice, ventral tegmental dopamine neurons exhibited enhanced spike timing–dependent long-term potentiation, altered amplitude of miniature postsynaptic currents and elevated dopamine in basal forebrain. Thus, AgRP neurons determine the set point of the reward circuitry and associated behaviors. PMID:22729177

SIMULTANEOUS ESTIMATION OF MOXIFLOXACIN HYDROCHLORIDE AND DEXAMETHASONE SODIUM PHOSPHATE IN BULK AND IN OPHTHALMIC SOLUTION BY RP- HPLC

OpenAIRE

DHUMAL, D. M; SHIRKHEDKAR, A. A; NERKAR, P. P; SURANA, S. J

2012-01-01

A new simple, precise, accurate and selective RP-HPLC method has been developed and validated for simultaneous estimation of Moxifloxacin Hydrochloride (MOX) and Dexamethasone Sodium Phosphate (DSP) in Ophthalmic Solution. The method was carried out on a Qualisil RP C-8 (250 mm x 4.6 mm, 5 µm) column with a mobile phase consisting of Methanol: Water (75:25 v/v) pH adjusted to 3.0 with ortho-phosphoric acid of aqueous phase and flow rate of 1.0 mL min¹. Detection was carried out at 240 nm. The...

Gs-coupled GPCR signalling in AgRP neurons triggers sustained increase in food intake.

Science.gov (United States)

Nakajima, Ken-ichiro; Cui, Zhenzhong; Li, Chia; Meister, Jaroslawna; Cui, Yinghong; Fu, Ou; Smith, Adam S; Jain, Shalini; Lowell, Bradford B; Krashes, Michael J; Wess, Jürgen

2016-01-08

Agouti-related peptide (AgRP) neurons of the hypothalamus play a key role in regulating food intake and body weight, by releasing three different orexigenic molecules: AgRP; GABA; and neuropeptide Y. AgRP neurons express various G protein-coupled receptors (GPCRs) with different coupling properties, including Gs-linked GPCRs. At present, the potential role of Gs-coupled GPCRs in regulating the activity of AgRP neurons remains unknown. Here we show that the activation of Gs-coupled receptors expressed by AgRP neurons leads to a robust and sustained increase in food intake. We also provide detailed mechanistic data linking the stimulation of this class of receptors to the observed feeding phenotype. Moreover, we show that this pathway is clearly distinct from other GPCR signalling cascades that are operative in AgRP neurons. Our data suggest that drugs able to inhibit this signalling pathway may become useful for the treatment of obesity.

Arcuate AgRP neurons and the regulation of energy balance

Directory of Open Access Journals (Sweden)

Céline eCansell

2012-12-01

Full Text Available The arcuate nucleus of the hypothalamus contains at least two crucial populations of neurons that continuously monitor signals reflecting energy status and promote the appropriate behavioral and metabolic responses to changes in energy demand. Neurons making pro-opiomelanocortin (POMC decrease food intake and increase energy expenditure through activation of G protein-coupled receptors melanocortin receptors (MCR via the release of a-melanocyte stimulating hormone. A prevailing idea until recently was that the neighboring neurons expressing the orexigenic neuropeptides, agouti-related protein (AgRP and neuropeptide Y (NPY (AgRP neurons increased feeding by opposing the anorexigenic actions of the POMC neurons. AgRP neurons activation but not POMC neurons inhibition was recently demonstrated to be necessary and sufficient to promote feeding. AgRP expressing axons were identified in mesolimbic, midbrain and pontine structure where they regulate feeding but also feeding-independent functions such as reward or peripheral nutrient partitioning. Post-synaptic Gamma aminobutyric acid (GABA, lasting in a timeline similar to neuromodulation, was identified as the core mechanism by which hunger-activated neurons regulate feeding and non-food related processes in a melanocortin independent manner.

Effect of TiO2 on conjugative transfer of RP4 plasmid

International Nuclear Information System (INIS)

Qian Di; Zhang Buchang; Yang Dong; Chen Zhaoli; Jin Min; Qiu Zhigang; Li Junwen

2013-01-01

Objective: To explore the effect and law of nano-titanium dioxide on the conjugative transfer of RP4 plasmid. Methods: Mating was conducted between Escherichia coli HB101 (RP4) and E. coli K12Rif in saline without stirring under certain conditions and the donor per recipient ratio was 1:1 constantly. The selective LB agar medium plates containing appropriate antibiotics were used to count the number of transconjugants and the conjugative transfer frequency. Results: Nano-titanium dioxide could promote the conjugative transfer of RP4. The nano-titanium dioxide concentration, bacterial concentration, mating temperature and mating time could affect the conjugative transfer of RP4. Conclusion: Nano-titanium dioxide can promote plasmid conjugal transfer in the liquid phase under certain conditions, which may pose a potential hazard to environmental and human health. (authors)

TCPTP Regulates Insulin Signalling in AgRP Neurons to Coordinate Glucose Metabolism with Feeding.

Science.gov (United States)

Dodd, Garron T; Lee-Young, Robert S; Brüning, Jens C; Tiganis, Tony

2018-04-30

Insulin regulates glucose metabolism by eliciting effects on peripheral tissues as well as the brain. Insulin receptor (IR) signalling inhibits AgRP-expressing neurons in the hypothalamus to contribute to the suppression of hepatic glucose production (HGP) by insulin, whereas AgRP neuronal activation attenuates brown adipose tissue (BAT) glucose uptake. The tyrosine phosphatase TCPTP suppresses IR signalling in AgRP neurons. Hypothalamic TCPTP is induced by fasting and degraded after feeding. Here we assessed the influence of TCPTP in AgRP neurons in the control of glucose metabolism. TCPTP deletion in AgRP neurons ( Agrp -Cre; Ptpn2 fl/fl ) enhanced insulin sensitivity as assessed by the increased glucose infusion rates and reduced HGP during hyperinsulinemic-euglycemic clamps, accompanied by increased [ 14 C]-2-deoxy-D-glucose uptake in BAT and browned white adipose tissue. TCPTP deficiency in AgRP neurons promoted the intracerebroventricular insulin-induced repression of hepatic gluconeogenesis in otherwise unresponsive food-restricted mice yet had no effect in fed/satiated mice where hypothalamic TCPTP levels are reduced. The improvement in glucose homeostasis in Agrp -Cre; Ptpn2 fl/fl mice was corrected by IR heterozygosity ( Agrp -Cre; Ptpn2 fl/fl ; Insr fl/+ ), causally linking the effects on glucose metabolism with the IR signalling in AgRP neurons. Our findings demonstrate that TCPTP controls IR signalling in AgRP neurons to coordinate HGP and brown/beige adipocyte glucose uptake in response to feeding/fasting. © 2018 by the American Diabetes Association.

BKP and projective Hurwitz numbers

Science.gov (United States)

Natanzon, Sergey M.; Orlov, Aleksandr Yu.

2017-06-01

We consider d-fold branched coverings of the projective plane RP^2 and show that the hypergeometric tau function of the BKP hierarchy of Kac and van de Leur is the generating function for weighted sums of the related Hurwitz numbers. In particular, we get the RP^2 analogues of the CP^1 generating functions proposed by Okounkov and by Goulden and Jackson. Other examples are Hurwitz numbers weighted by the Hall-Littlewood and by the Macdonald polynomials. We also consider integrals of tau functions which generate Hurwitz numbers related to base surfaces with arbitrary Euler characteristics sc {e}, in particular projective Hurwitz numbers sc {e}=1.

Development and Validation of a UV Spectrophotometric and a RP-HPLC Methods for Moexipril Hydrochloride in Pure Form and Pharmaceutical Dosage Form

International Nuclear Information System (INIS)

Mastiholimath, V.S.; Gupte, P.P.; Mannur, V.S.

2012-01-01

A simple and reliable UV spectrophotometric and high-performance liquid chromatography (HPLC) methods were developed and validated for Moexipril hydrochloride in pure form and pharmaceutical dosage form. The RP-HPLC method was developed on agilant eclipse C 18 , (150 mm x 4.6 mm, 5 μm) with a mobile phase gradient system of 60 % (methanol:acetonitrile (70:30 % v/v)) : 40 % 20 mM ammonium acetate buffer pH 4.5 (v/v) and UV spectrophotometric method was developed in phosphate buffer pH 6.8. The effluent was monitored by SPD-M20A, prominence PDA detector at 210 nm. Calibration curve was linear over the concentration range of 10-35 μg/ml and 1-9 μg/ml for RP-HPLC and UV with a regression coefficient of 0.999. For RP-HPLC method Inter-day and intra-day precision % RSD values were found to be 1.00078 % and 1.49408 % respectively. For UV method 0.73386 % to 1.44111 % for inter day 0.453864 to 1.15542 intra-day precision. Recovery of Moexipril hydrochloride was found to be in the range of 99.8538 % to 101.5614 % and 100.5297586 % to 100.6431587 % for UV and RP-HPLC respectively. The limits of detection (LOD) and quantification (LOQ) for HPLC were 0.98969 and 2.99907 μg/ml, respectively. The developed RP-HPLC and UV spectrophotometric method was successfully applied for the quantitative determination of Moexipril hydrochloride in pharmaceutical dosage. (author)

Characterization of phytoplasmas related to 'Candidatus Phytoplasma asteris' subgroup rpI-L in Iran

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Vali-Sichani Fereshteh

2014-07-01

Full Text Available In two of Iran's central provinces, several herbaceous plants showing phytoplasma disease symptoms were collected to detect 'Canididatus Phytoplasma asteris'-related phytoplasmas. Confirmation of an association of phytoplasmas with diseased plants was done using polymerase chain reaction (PCR assays having the phytoplasma universal primer pairs P1/P7 followed by R16F2n/ R16R2 in nested PCR. Then, for detection of 'Ca. P. asteris', DNA samples were subjected to amplification of rp and tuf genes using specific primer pairs rp(IF1A/rp(IR1A and fTufAy/rTufAy, respectively. Restriction fragment length polymorphism or RFLP analyses of rp gene fragments using Tsp509I restriction enzyme as well as sequence analyses indicated that 'Ca. P. asteris'-related phytoplasmas associated with carrot, niger seed and scallion plants in these regions, belong to the rpI-L subgroup. This research is the first report of carrot, niger seed, and scallion infection with phytoplasmas belonging to the rpI-L subgroup.

Arcuate hypothalamic AgRP and putative POMC neurons show opposite changes in spiking across multiple timescales

Science.gov (United States)

Mandelblat-Cerf, Yael; Ramesh, Rohan N; Burgess, Christian R; Patella, Paola; Yang, Zongfang; Lowell, Bradford B; Andermann, Mark L

2015-01-01

Agouti-related-peptide (AgRP) neurons—interoceptive neurons in the arcuate nucleus of the hypothalamus (ARC)—are both necessary and sufficient for driving feeding behavior. To better understand the functional roles of AgRP neurons, we performed optetrode electrophysiological recordings from AgRP neurons in awake, behaving AgRP-IRES-Cre mice. In free-feeding mice, we observed a fivefold increase in AgRP neuron firing with mounting caloric deficit in afternoon vs morning recordings. In food-restricted mice, as food became available, AgRP neuron firing dropped, yet remained elevated as compared to firing in sated mice. The rapid drop in spiking activity of AgRP neurons at meal onset may reflect a termination of the drive to find food, while residual, persistent spiking may reflect a sustained drive to consume food. Moreover, nearby neurons inhibited by AgRP neuron photostimulation, likely including satiety-promoting pro-opiomelanocortin (POMC) neurons, demonstrated opposite changes in spiking. Finally, firing of ARC neurons was also rapidly modulated within seconds of individual licks for liquid food. These findings suggest novel roles for antagonistic AgRP and POMC neurons in the regulation of feeding behaviors across multiple timescales. DOI: http://dx.doi.org/10.7554/eLife.07122.001 PMID:26159614

PGC-1α expression in murine AgRP neurons regulates food intake and energy balance

Directory of Open Access Journals (Sweden)

Jonathan F. Gill

2016-07-01

Full Text Available Objective: Food intake and whole-body energy homeostasis are controlled by agouti-related protein (AgRP and pro-opiomelanocortin (POMC neurons located in the arcuate nucleus of the hypothalamus. Key energy sensors, such as the AMP-activated protein kinase (AMPK or sirtuin 1 (SIRT1, are essential in AgRP and POMC cells to ensure proper energy balance. In peripheral tissues, the transcriptional coactivator PGC-1α closely associates with these sensors to regulate cellular metabolism. The role of PGC-1α in the ARC nucleus, however, remains unknown. Methods: Using AgRP and POMC neurons specific knockout (KO mouse models we studied the consequences of PGC-1α deletion on metabolic parameters during fed and fasted states and on ghrelin and leptin responses. We also took advantage of an immortalized AgRP cell line to assess the impact of PGC-1α modulation on fasting induced AgRP expression. Results: PGC-1α is dispensable for POMC functions in both fed and fasted states. In stark contrast, mice carrying a specific deletion of PGC-1α in AgRP neurons display increased adiposity concomitant with significantly lower body temperature and RER values during nighttime. In addition, the absence of PGC-1α in AgRP neurons reduces food intake in the fed and fasted states and alters the response to leptin. Finally, both in vivo and in an immortalized AgRP cell line, PGC-1α modulates AgRP expression induction upon fasting. Conclusions: Collectively, our results highlight a role for PGC-1α in the regulation of AgRP neuronal functions in the control of food intake and peripheral metabolism. Author Video: Author Video Watch what authors say about their articles Keywords: PGC-1α, Agouti-related protein, Metabolism, Energy homeostasis, Pro-opiomelanocortin, Transcriptional regulation

Argentine Radiation Protection Society Experience in RP education and training

International Nuclear Information System (INIS)

Bomben, A. M.; Ciallella, N. R.; Thomasz, E.; Rudelli, M.; Gisone, P.; Ventura, M.; Gomez Parada, I.; Signoretta, C.

2003-01-01

Since its creation in 1967, the Argentine Radiation Protection Society (SAR) promotes all the radiation protection and nuclear safety aspects not only within the scientific, technical and academic areas, but also to general public. To fulfill this objective, SAR organised training and refresher courses, seminars and workshops on RP subjects. During 2002, SAR organised 7 basic and specialized courses regarding the uses of radioactive materials in industrial applications and the course on medical response in radiological accidents, that was attended by Argentine and other Latin American participants. The programmes of the courses are developed in compliance with the legal requirements and also considering specifics needs. In this paper, the characteristics of the courses are enunciated and basic statistics regarding courses and participants are presented. For the 2003 and 2004, SAR foresees the organisation of 18 courses per year and has the capacity to deliver other courses by request. all the courses are delivered in Spanish language. Based on this educational experience SAR consider a priority the inclusion, of a RP module in all the scientific graduate programmes to generate awareness on the importance of RP. Taking into account the migration of professionals to Europe and North America and the Globalization, SAR advocates the harmonization of RP syllabus to attain an international recognition. (Author)

A Small Potassium Current in AgRP/NPY Neurons Regulates Feeding Behavior and Energy Metabolism.

Science.gov (United States)

He, Yanlin; Shu, Gang; Yang, Yongjie; Xu, Pingwen; Xia, Yan; Wang, Chunmei; Saito, Kenji; Hinton, Antentor; Yan, Xiaofeng; Liu, Chen; Wu, Qi; Tong, Qingchun; Xu, Yong

2016-11-08

Neurons that co-express agouti-related peptide (AgRP) and neuropeptide Y (NPY) are indispensable for normal feeding behavior. Firing activities of AgRP/NPY neurons are dynamically regulated by energy status and coordinate appropriate feeding behavior to meet nutritional demands. However, intrinsic mechanisms that regulate AgRP/NPY neural activities during the fed-to-fasted transition are not fully understood. We found that AgRP/NPY neurons in satiated mice express high levels of the small-conductance calcium-activated potassium channel 3 (SK3) and are inhibited by SK3-mediated potassium currents; on the other hand, food deprivation suppresses SK3 expression in AgRP/NPY neurons, and the decreased SK3-mediated currents contribute to fasting-induced activation of these neurons. Genetic mutation of SK3 specifically in AgRP/NPY neurons leads to increased sensitivity to diet-induced obesity, associated with chronic hyperphagia and decreased energy expenditure. Our results identify SK3 as a key intrinsic mediator that coordinates nutritional status with AgRP/NPY neural activities and animals' feeding behavior and energy metabolism. Copyright © 2016 The Author(s). Published by Elsevier Inc. All rights reserved.

76 FR 35006 - Recovery Policy RP9523.4, Demolition of Private Structures

Science.gov (United States)

2011-06-15

...] Recovery Policy RP9523.4, Demolition of Private Structures AGENCY: Federal Emergency Management Agency, DHS... (FEMA) is accepting comments on Recovery Policy RP9523.4, Demolition of Private Structures. DATES... guidance in determining the eligibility of demolition of private structures under the provisions of the...

75 FR 67165 - Proposed Collection; Comment Request for Revenue Procedure2007-XX (RP-155430-05)

Science.gov (United States)

2010-11-01

... Revenue Procedure 2007- XX (RP-155430-05) AGENCY: Internal Revenue Service (IRS), Treasury. ACTION: Notice... soliciting comments concerning Revenue Procedure 2007-XX (RP-155430-05), Section 6707/6707A Accelerated... Procedure 2007-XX (RP-155430-05). Abstract: The collection of information this revenue procedure requires is...

RP process studies with radioactive beams at ATLAS

Energy Technology Data Exchange (ETDEWEB)

Rehm, K E [Argonne National Lab., Physics Div., Argonne, IL (United States)

1998-06-01

Reactions of interest to nuclear astrophysics have been studied with radioactive beams at the ATLAS accelerator. Using a modified ISOL technique, beams of {sup 18}F(T{sub 1/2}=110 min) and {sup 56}Ni(T{sub 1/2}=6.1 d) have been produced. The reactions {sup 18}F(p,{alpha}){sup 15}O, {sup 18}F(p,{gamma}){sup 19}Ne, and {sup 56}Ni(d,p){sup 57}Ni have been investigated. The results indicate that the {sup 18}F(p,{gamma}) route is a small contributor to the breakout from the hot CNO cycle into the rp process, while the {sup 56}Ni(p,{gamma}){sup 57}Cu rate is about ten times larger than previously assumed. (orig.)

[3H]52770 RP, a platelet-activating factor receptor antagonist, and tritiated platelet-activating factor label a common specific binding site in human polymorphonuclear leukocytes

International Nuclear Information System (INIS)

Marquis, O.; Robaut, C.; Cavero, I.

1988-01-01

In human polymorphonuclear leukocytes (PMNs), the tritiated platelet activating factor ([ 3 H]PAF) labels in a saturable manner a single class of binding sites with a Kd of 3.5 +/- 0.5 nM (n = 7) and a maximum binding capacity (Bmax) of 206 +/- 13 fmol/2.5 X 10(6) PMNs (n = 7). 52770 RP, a nonphospholipid antagonist of PAF receptors, fully and competitively displaced the [ 3 H]PAF from its binding sites with a Ki of 7.0 +/- 0.7 nM (n = 4). The high potency and the low solubility in cellular membranes of this compound led us to prepare [ 3 H]52770 RP. This ligand was characterized by a binding which was rapid, reversible, confined to a single site, saturable, specific and stereoselective. Its Kd and Bmax were 4.2 +/- 0.3 nM and 181 +/- 11 fmol/2.5 X 10(6) PMNs, respectively. The stereoselectivity of the binding was suggested by the 600- and 1050-fold higher potency of the d-enantiomer with respect to l-52770 RP in displacing [ 3 H]52770 RP or [ 3 H]PAF, respectively. Several PAF analogs (e.g., lyso-PAF, 2-O-methyl-lyso-PAF), which are poorly active as PAF receptor agonists in functional tests, were weak displacers of [ 3 H]PAF and [ 3 H]52770 RP. Furthermore, for a series of 14 known PAF receptor agonists or antagonists belonging to different chemical families, there was an excellent correlation (r = 0.98) between their ability to displace [ 3 H]PAF and [ 3 H]52770 RP. Thus, [ 3 H]52770 RP and [ 3 H]PAF appear to interact with the same binding site on human PMNs which is proposed to be the PAF receptor mediating functional responses

75 FR 49508 - Recovery Policy, RP9525.7, Labor Costs-Emergency Work

Science.gov (United States)

2010-08-13

...] Recovery Policy, RP9525.7, Labor Costs--Emergency Work AGENCY: Federal Emergency Management Agency, DHS... (FEMA) is accepting comments on RP9525.7, Labor Costs--Emergency Work. This is an existing policy that is scheduled for review to ensure that Recovery Directorate policies are up to date, incorporate...

Mutations in REEP6 Cause Autosomal-Recessive Retinitis Pigmentosa.

Science.gov (United States)

Arno, Gavin; Agrawal, Smriti A; Eblimit, Aiden; Bellingham, James; Xu, Mingchu; Wang, Feng; Chakarova, Christina; Parfitt, David A; Lane, Amelia; Burgoyne, Thomas; Hull, Sarah; Carss, Keren J; Fiorentino, Alessia; Hayes, Matthew J; Munro, Peter M; Nicols, Ralph; Pontikos, Nikolas; Holder, Graham E; Asomugha, Chinwe; Raymond, F Lucy; Moore, Anthony T; Plagnol, Vincent; Michaelides, Michel; Hardcastle, Alison J; Li, Yumei; Cukras, Catherine; Webster, Andrew R; Cheetham, Michael E; Chen, Rui

2016-12-01

Retinitis pigmentosa (RP) is the most frequent form of inherited retinal dystrophy. RP is genetically heterogeneous and the genes identified to date encode proteins involved in a wide range of functional pathways, including photoreceptor development, phototransduction, the retinoid cycle, cilia, and outer segment development. Here we report the identification of biallelic mutations in Receptor Expression Enhancer Protein 6 (REEP6) in seven individuals with autosomal-recessive RP from five unrelated families. REEP6 is a member of the REEP/Yop1 family of proteins that influence the structure of the endoplasmic reticulum but is relatively unstudied. The six variants identified include three frameshift variants, two missense variants, and a genomic rearrangement that disrupts exon 1. Human 3D organoid optic cups were used to investigate REEP6 expression and confirmed the expression of a retina-specific isoform REEP6.1, which is specifically affected by one of the frameshift mutations. Expression of the two missense variants (c.383C>T [p.Pro128Leu] and c.404T>C [p.Leu135Pro]) and the REEP6.1 frameshift mutant in cultured cells suggest that these changes destabilize the protein. Furthermore, CRISPR-Cas9-mediated gene editing was used to produce Reep6 knock-in mice with the p.Leu135Pro RP-associated variant identified in one RP-affected individual. The homozygous knock-in mice mimic the clinical phenotypes of RP, including progressive photoreceptor degeneration and dysfunction of the rod photoreceptors. Therefore, our study implicates REEP6 in retinal homeostasis and highlights a pathway previously uncharacterized in retinal dystrophy. Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.

Neutronic calculation of safety parameters for the RP-0 and RP-10 nuclear reactors

OpenAIRE

Lázaro, Gerardo; Deen, James R.; Woodruff, William L.

2002-01-01

Theoretical safety calculations were done with proved codes utilized by the staff of the RERTR program in the HEU to LEU core conversions. The studies were designed to evaluate the reactivity coefficients and kinetics parameters of the reactor involved in the evolution of peak power transients by reactivity insertion accidents. It was done to show the trend of these reactivity coefficients as a function of the core size and fuel depletion for RP10 cores. It was useful to get a better underst...

77 FR 40626 - RP9580.210, Personal Assistance Services in Shelters Fact Sheet

Science.gov (United States)

2012-07-10

... DEPARTMENT OF HOMELAND SECURITY Federal Emergency Management Agency [Docket ID FEMA-2012-0014] RP9580.210, Personal Assistance Services in Shelters Fact Sheet AGENCY: Federal Emergency Management... Management Agency (FEMA) is accepting comments on Recovery Fact Sheet RP9580.210, Personal Assistance...

The Relevance of AgRP Neuron-Derived GABA Inputs to POMC Neurons Differs for Spontaneous and Evoked Release.

Science.gov (United States)

Rau, Andrew R; Hentges, Shane T

2017-08-02

Hypothalamic agouti-related peptide (AgRP) neurons potently stimulate food intake, whereas proopiomelanocortin (POMC) neurons inhibit feeding. Whether AgRP neurons exert their orexigenic actions, at least in part, by inhibiting anorexigenic POMC neurons remains unclear. Here, the connectivity between GABA-releasing AgRP neurons and POMC neurons was examined in brain slices from male and female mice. GABA-mediated spontaneous IPSCs (sIPSCs) in POMC neurons were unaffected by disturbing GABA release from AgRP neurons either by cell type-specific deletion of the vesicular GABA transporter or by expression of botulinum toxin in AgRP neurons to prevent vesicle-associated membrane protein 2-dependent vesicle fusion. Additionally, there was no difference in the ability of μ-opioid receptor (MOR) agonists to inhibit sIPSCs in POMC neurons when MORs were deleted from AgRP neurons, and activation of the inhibitory designer receptor hM4Di on AgRP neurons did not affect sIPSCs recorded from POMC neurons. These approaches collectively indicate that AgRP neurons do not significantly contribute to the strong spontaneous GABA input to POMC neurons. Despite these observations, optogenetic stimulation of AgRP neurons reliably produced evoked IPSCs in POMC neurons, leading to the inhibition of POMC neuron firing. Thus, AgRP neurons can potently affect POMC neuron function without contributing a significant source of spontaneous GABA input to POMC neurons. Together, these results indicate that the relevance of GABAergic inputs from AgRP to POMC neurons is state dependent and highlight the need to consider different types of transmitter release in circuit mapping and physiologic regulation. SIGNIFICANCE STATEMENT Agouti-related peptide (AgRP) neurons play an important role in driving food intake, while proopiomelanocortin (POMC) neurons inhibit feeding. Despite the importance of these two well characterized neuron types in maintaining metabolic homeostasis, communication between these

Ice Sheet Model Intercomparison Project (ISMIP6) Contribution to CMIP6

Science.gov (United States)

Nowicki, Sophie M. J.; Payne, Tony; Larour, Eric; Seroussi, Helene; Goelzer, Heiko; Lipscomb, William; Gregory, Jonathan; Abe-Ouchi, Ayako; Shepherd, Andrew

2016-01-01

Reducing the uncertainty in the past, present, and future contribution of ice sheets to sea-level change requires a coordinated effort between the climate and glaciology communities. The Ice Sheet Model Intercomparison Project for CMIP6 (ISMIP6) is the primary activity within the Coupled Model Intercomparison Project phase 6 (CMIP6) focusing on the Greenland and Antarctic ice sheets. In this paper, we describe the framework for ISMIP6 and its relationship with other activities within CMIP6. The ISMIP6 experimental design relies on CMIP6 climate models and includes, for the first time within CMIP, coupled ice-sheetclimate models as well as standalone ice-sheet models. To facilitate analysis of the multi-model ensemble and to generate a set of standard climate inputs for standalone ice-sheet models, ISMIP6 defines a protocol for all variables related to ice sheets. ISMIP6 will provide a basis for investigating the feedbacks, impacts, and sea-level changes associated with dynamic ice sheets and for quantifying the uncertainty in ice-sheet-sourced global sea-level change.

Ice Sheet Model Intercomparison Project (ISMIP6) contribution to CMIP6

Science.gov (United States)

Nowicki, Sophie M.J.; Payne, Tony; Larour, Eric; Seroussi, Helene; Goelzer, Heiko; Lipscomb, William; Gregory, Jonathan; Abe-Ouchi, Ayako; Shepherd, Andrew

2018-01-01

Reducing the uncertainty in the past, present and future contribution of ice sheets to sea-level change requires a coordinated effort between the climate and glaciology communities. The Ice Sheet Model Intercomparison Project for CMIP6 (ISMIP6) is the primary activity within the Coupled Model Intercomparison Project – phase 6 (CMIP6) focusing on the Greenland and Antarctic Ice Sheets. In this paper, we describe the framework for ISMIP6 and its relationship to other activities within CMIP6. The ISMIP6 experimental design relies on CMIP6 climate models and includes, for the first time within CMIP, coupled ice sheet – climate models as well as standalone ice sheet models. To facilitate analysis of the multi-model ensemble and to generate a set of standard climate inputs for standalone ice sheet models, ISMIP6 defines a protocol for all variables related to ice sheets. ISMIP6 will provide a basis for investigating the feedbacks, impacts, and sea-level changes associated with dynamic ice sheets and for quantifying the uncertainty in ice-sheet-sourced global sea-level change. PMID:29697697

Determination of Critical Properties of Endothermic Hydrocarbon Fuel RP-3 Based on Flow Visualization

Science.gov (United States)

Wang, Ning; Zhou, Jin; Pan, Yu; Wang, Hui

2014-01-01

The critical pressure and temperature of an endothermic hydrocarbon fuel RP-3 were determined by flow visualization. The flow pattern images of RP-3 at different pressures and temperatures were obtained. The critical pressure is identified by disappearance of the phase change while the critical temperature is determined by appearance of the opalescence phenomenon under the critical pressure. The opalescence phenomenon is unique to the critical point. The critical pressure and temperature of RP-3 are determined to be 2.3 MPa and 646 K, respectively.

The mitochondrial SIR2 related protein 2 (SIR2RP2 impacts Leishmania donovani growth and infectivity.

Directory of Open Access Journals (Sweden)

Nimisha Mittal

2017-05-01

Full Text Available Leishmania donovani, a protozoan parasite is the major causative agent of visceral leishmaniasis. Increased toxicity and resistance to the existing repertoire of drugs has been reported. Hence, an urgent need exists for identifying newer drugs and drug targets. Previous reports have shown sirtuins (Silent Information Regulator from kinetoplastids as promising drug targets. Leishmania species code for three SIR2 (Silent Information Regulator related proteins. Here, we for the first time report the functional characterization of SIR2 related protein 2 (SIR2RP2 of L. donovani.Recombinant L. donovani SIR2RP2 was expressed in E. coli and purified. The enzymatic functions of SIR2RP2 were determined. The subcellular localization of LdSIR2RP2 was done by constructing C-terminal GFP-tagged full-length LdSIR2RP2. Deletion mutants of LdSIR2RP2 were generated in Leishmania by double targeted gene replacement methodology. These null mutants were tested for their proliferation, virulence, cell cycle defects, mitochondrial functioning and sensitivity to known SIR2 inhibitors.Our data suggests that LdSIR2RP2 possesses NAD+-dependent ADP-ribosyltransferase activity. However, NAD+-dependent deacetylase and desuccinylase activities were not detected. The protein localises to the mitochondrion of the promastigotes. Gene deletion studies showed that ΔLdSIR2RP2 null mutants had restrictive growth phenotype associated with accumulation of cells in the G2/M phase and compromised mitochondrial functioning. The null mutants had attenuated infectivity. Deletion of LdSIR2RP2 resulted in increased sensitivity of the parasites to the known SIR2 inhibitors. The sirtuin inhibitors inhibited the ADP-ribosyltransferase activity of recombinant LdSIR2RP2. In conclusion, sirtuins could be used as potential new drug targets for visceral leishmaniasis.

Nucleosynthesis of proton-rich nuclei. Experimental results on the rp-process

International Nuclear Information System (INIS)

Galaviz, D; Amthor, A M; Bazin, D; Becerril, A D; Brown, B A; Cole, A; Cook, J M; Elliot, T; Estrade, A; Gade, A; Glasmacher, T; Lorusso, G; Matos, M; Montes, F; Mueller, W; Chen, A A; Fueloep, Z S; Heger, A; Howard, M E; Kessler, R

2010-01-01

We report in this study the nuclear properties of proton-rich isotopes located along the rp-process path. The experiments have recently been performed at the National Superconducting Cyclotron Laboratory (NSCL) at Michigan State University. The level properties above the proton separation energy of the nuclei 30 S, 36 K and 37 Ca were measured with precision of < 10 keV. This will allow a reduction in the determination of the astrophysical (p,γ) reaction rate under rp-process conditions.

Isolation, Characterization, and RP-HPLC Estimation of P-Coumaric Acid from Methanolic Extract of Durva Grass (Cynodon dactylon Linn. (Pers.

Directory of Open Access Journals (Sweden)

Ramadoss Karthikeyan

2015-01-01

Full Text Available P-coumaric acid is a nonflavonoid phenolic acid and is a major constituent of the species Cynodon dactylon Linn. (Pers.. In this study isolation of P-coumaric acid was achieved by preparative TLC and the compound thus isolated was characterised by UV, mass, and H1 NMR spectral analysis. An isocratic RP-HPLC method was developed for the estimation of P-coumaric acid from methanolic extracts of durva grass. The chromatographic separations were achieved by RP-C18 column (250 mm × 4.6 mm, 5 μ, Shimadzu LC-20AT Prominence liquid chromatograph, and a mobile phase composed of water : methanol : glacial acetic acid (65 : 34 : 1 v/v. The flow rate was 1.0 mL/min and the analyses of column effluents were performed using UV-visible detector at 310 nm. Retention time of P-coumaric acid was found to be 6.617 min. This method has obeyed linearity over the concentration range of 2–10 μg/mL and the regression coefficient obtained from linearity plot for P-coumaric acid was found to be 0.999. RP-HPLC method was validated in pursuance of ICH guidelines.

Isolation, Characterization, and RP-HPLC Estimation of P-Coumaric Acid from Methanolic Extract of Durva Grass (Cynodon dactylon Linn.) (Pers.)

Science.gov (United States)

Karthikeyan, Ramadoss; Devadasu, Chapala; Srinivasa Babu, Puttagunta

2015-01-01

P-coumaric acid is a nonflavonoid phenolic acid and is a major constituent of the species Cynodon dactylon Linn. (Pers.). In this study isolation of P-coumaric acid was achieved by preparative TLC and the compound thus isolated was characterised by UV, mass, and H1 NMR spectral analysis. An isocratic RP-HPLC method was developed for the estimation of P-coumaric acid from methanolic extracts of durva grass. The chromatographic separations were achieved by RP-C18 column (250 mm × 4.6 mm, 5 μ), Shimadzu LC-20AT Prominence liquid chromatograph, and a mobile phase composed of water : methanol : glacial acetic acid (65 : 34 : 1 v/v). The flow rate was 1.0 mL/min and the analyses of column effluents were performed using UV-visible detector at 310 nm. Retention time of P-coumaric acid was found to be 6.617 min. This method has obeyed linearity over the concentration range of 2–10 μg/mL and the regression coefficient obtained from linearity plot for P-coumaric acid was found to be 0.999. RP-HPLC method was validated in pursuance of ICH guidelines. PMID:25788944

Development and validation of an rp-hplc method for simultaneous determination of Ramipril and Amlodipine in tablets

Directory of Open Access Journals (Sweden)

Shi-Ying Dai

2013-12-01

Full Text Available An rp-hplc method for the simultaneous determination of Ramipril (RP and Amlodipine (AL in tablets was developed and validated by Chinese Pharmacopoeia 2010. The linearity of the proposed method was investigated in the range of 0.01–0.25 mg/mL (r2=0.9998 for RP and 0.014–0.36 mg/mL (r2=0.9997 for AL. The limits of detection (LOD were 0.06 μg/mL and 0.02 μg/mL for RP and AL, and the limits of quantitation (LOQ were 0.2 μg/mL and 0.07 μg/mL, respectively. Some major impurities and degradation products did not disturb the detection of RP and AL and the assay can thus be considered stability-indicating. Keywords: Ramipril, Amlodipine, RP-HPLC, Stability-indicating

Burnup measurements on spent fuel elements of the RP-10 research reactor

Energy Technology Data Exchange (ETDEWEB)

Vela Mora, Mariano; Gallardo Padilla, Alberto; Palomino, Jose Luis Castro, E-mail: mvela@ipen.gob.p [Instituto Peruano de Energia Nuclear (IPEN/Peru), Lima (Peru). Grupo de Calculo, Analisis y Seguridad de Reactores; Terremoto, Luis Antonio Albiac, E-mail: laaterre@ipen.b [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)

2011-07-01

This work describes the measurement, using nondestructive gamma-ray spectroscopy, of the average burnup attained by Material Testing Reactor (MTR) fuel elements irradiated in the RP-10 research reactor. Measurements were performed at the reactor storage pool area using {sup 137}Cs as the only burnup monitor, even for spent fuel elements with cooling times much shorter than two years. The experimental apparatus was previously calibrated in efficiency to obtain absolute average burnup values, which were compared against corresponding ones furnished by reactor physics calculations. The mean deviation between both values amounts to 6%. (author)

Burnup measurements on spent fuel elements of the RP-10 research reactor

International Nuclear Information System (INIS)

Vela Mora, Mariano; Gallardo Padilla, Alberto; Palomino, Jose Luis Castro

2011-01-01

This work describes the measurement, using nondestructive gamma-ray spectroscopy, of the average burnup attained by Material Testing Reactor (MTR) fuel elements irradiated in the RP-10 research reactor. Measurements were performed at the reactor storage pool area using 137 Cs as the only burnup monitor, even for spent fuel elements with cooling times much shorter than two years. The experimental apparatus was previously calibrated in efficiency to obtain absolute average burnup values, which were compared against corresponding ones furnished by reactor physics calculations. The mean deviation between both values amounts to 6%. (author)

Differential benefits of rock phosphate (RP) by tomato ...

African Journals Online (AJOL)

plant as affected by nitrogen froms and soil types. ... Moneymaker in minirhizotrons at Hohenheim to assess root-induced chemical changes in the ... from rhizosphere pH treatment with C-horizon of Luvisol and there was no RP benefit to plant.

Accessible silanol sites - beneficial for the RP-HPLC separation of constitutional and diastereomeric azaspirovesamicol isomers.

Science.gov (United States)

Wenzel, Barbara; Fischer, Steffen; Brust, Peter; Steinbach, Jörg

2010-12-10

Different RP-HPLC columns (phenyl, conventional ODS, cross-linked C(18) and special end-capped C(8) and C(18) phases) were used to investigate the separation of four basic ionizable isomers. Using ACN/20mM NH(4)OAc aq., a separation was observed exclusively on RP columns with higher silanol activity at unusual high ACN concentration, indicating cation-exchange as main retention mechanism. Using MeOH/20mM NH(4)OAc aq., another separation at low MeOH concentrations was observed on both, RP columns with higher as well as RP columns with lower silanol activity, which is mainly based on hydrophobic interactions. The isomers were also separated on a bare silica column at higher MeOH content using NH(4)OAc. Since cation-exchange governs this retention, the elution order was different compared to the RP phases. A strong retention on the silica column was observed in ACN, which could be attributed to partition processes as additional retention mechanism. Copyright © 2010 Elsevier B.V. All rights reserved.

Autophagy in hypothalamic AgRP neurons regulates food intake and energy balance

OpenAIRE

Kaushik, Susmita; Rodriguez-Navarro, Jose Antonio; Arias, Esperanza; Kiffin, Roberta; Sahu, Srabani; Schwartz, Gary J.; Cuervo, Ana Maria; Singh, Rajat

2011-01-01

Macroautophagy is a lysosomal degradative pathway that maintains cellular homeostasis by turning over cellular components. Here, we demonstrate a role for autophagy in hypothalamic agouti-related peptide (AgRP) neurons in the regulation of food intake and energy balance. We show that starvation-induced hypothalamic autophagy mobilizes neuron-intrinsic lipids to generate endogenous free fatty acids, which in turn regulate AgRP levels. The functional consequences of inhibiting autophagy are the...

An excitatory paraventricular nucleus to AgRP neuron circuit that drives hunger.

Science.gov (United States)

Krashes, Michael J; Shah, Bhavik P; Madara, Joseph C; Olson, David P; Strochlic, David E; Garfield, Alastair S; Vong, Linh; Pei, Hongjuan; Watabe-Uchida, Mitsuko; Uchida, Naoshige; Liberles, Stephen D; Lowell, Bradford B

2014-03-13

Hunger is a hard-wired motivational state essential for survival. Agouti-related peptide (AgRP)-expressing neurons in the arcuate nucleus (ARC) at the base of the hypothalamus are crucial to the control of hunger. They are activated by caloric deficiency and, when naturally or artificially stimulated, they potently induce intense hunger and subsequent food intake. Consistent with their obligatory role in regulating appetite, genetic ablation or chemogenetic inhibition of AgRP neurons decreases feeding. Excitatory input to AgRP neurons is important in caloric-deficiency-induced activation, and is notable for its remarkable degree of caloric-state-dependent synaptic plasticity. Despite the important role of excitatory input, its source(s) has been unknown. Here, through the use of Cre-recombinase-enabled, cell-specific neuron mapping techniques in mice, we have discovered strong excitatory drive that, unexpectedly, emanates from the hypothalamic paraventricular nucleus, specifically from subsets of neurons expressing thyrotropin-releasing hormone (TRH) and pituitary adenylate cyclase-activating polypeptide (PACAP, also known as ADCYAP1). Chemogenetic stimulation of these afferent neurons in sated mice markedly activates AgRP neurons and induces intense feeding. Conversely, acute inhibition in mice with caloric-deficiency-induced hunger decreases feeding. Discovery of these afferent neurons capable of triggering hunger advances understanding of how this intense motivational state is regulated.

History of Sulphur Content Effects on the Thermal Stability of RP-1 under Heated Conditions

National Research Council Canada - National Science Library

Irvine, Solveig

2004-01-01

.... Since the main fuel used in modern liquid hydrocarbon systems is RP-1, there is concern that Standard Grade RP-1 may not be a suitable propellant for future-generation rocket engines due to concern...

Validated RP-HPLC Method for Quantification of Phenolic ...

African Journals Online (AJOL)

Purpose: To evaluate the total phenolic content and antioxidant potential of the methanol extracts of aerial parts and roots of Thymus sipyleus Boiss and also to determine some phenolic compounds using a newly developed and validated reversed phase high performance liquid chromatography (RP-HPLC) method.

Development and validation of polar RP-HPLC method for screening for ectoine high-yield strains in marine bacteria with green chemistry.

Science.gov (United States)

Chen, Jun; Chen, Jianwei; Wang, Sijia; Zhou, Guangmin; Chen, Danqing; Zhang, Huawei; Wang, Hong

2018-04-02

A novel, green, rapid, and precise polar RP-HPLC method has been successfully developed and screened for ectoine high-yield strain in marine bacteria. Ectoine is a polar and extremely useful solute which allows microorganisms to survive in extreme environmental salinity. This paper describes a polar-HPLC method employed polar RP-C18 (5 μm, 250 × 4.6 mm) using pure water as the mobile phase and a column temperature of 30 °C, coupled with a flow rate at 1.0 mL/min and detected under a UV detector at wavelength of 210 nm. Our method validation demonstrates excellent linearity (R 2  = 0.9993), accuracy (100.55%), and a limit of detection LOQ and LOD of 0.372 and 0.123 μgmL -1 , respectively. These results clearly indicate that the developed polar RP-HPLC method for the separation and determination of ectoine is superior to earlier protocols.

[Studies on fingerprinting of Flos Buddleja by RP-HPLC].

Science.gov (United States)

Han, Peng; Cui, Ya-jun; Guo, Hong-zhu; Guo, De-an

2004-10-01

To establish fingerprinting of Flos Buddleja by using RP-HPLC for the quality control. The HPLC condition was as follows: Inertsil ODS-3 C18 analytical column (4.6 mm x 250 mm, 5 microm), gredient eluation with MeCN (0.1% TFA)-H2O (0.1%TFA), flow rate 1.0 mL x min(-1), detection wavelength 254 nm. 10 commercial samples were analyzed to establish a fingerprinting. Among the obtained fingerprinting, most of the detected peaks were separated effectively. The accuracy, repeatability and stability of this method were satisfied. The RSDs of relative retention time and area of aimed peaks which existed in all samples wereless than 5%. Theresults were in accordance with the request of fingerprinting. The established fingerprinting can be used for the quality control of Flos Buddleja.

Valuation of the environmental impacts of the Kayraktepe dam/hydroelectric project, Turkey: An exercise in contingent valuation

International Nuclear Information System (INIS)

Biro, Y.E.K.

1998-01-01

This paper describes and evaluates the environmental impacts of the Kayraktepe Dam and Hydroelectric Power Plant in Turkey. The contingent valuation method is used to estimate the project's local environmental costs. The average local rural annual willingness to pay for the restoration of the environmental impacts of the project is found to be USD 300 person -1 . Loss of forestry and agricultural benefits from the reservoir area that will be inundated are estimated to be USD 12.66 mill. yr -1 . When the Kayraktepe Project's external costs are internalized, its benefit cost ratio falls from 1.35 to 0.84, indicating that the project is economically undesirable and the decision for its construction needs to be reconsidered 26 refs, 1 fig, 5 tabs

Novel USH2A compound heterozygous mutations cause RP/USH2 in a Chinese family.

Science.gov (United States)

Liu, Xiaowen; Tang, Zhaohui; Li, Chang; Yang, Kangjuan; Gan, Guanqi; Zhang, Zibo; Liu, Jingyu; Jiang, Fagang; Wang, Qing; Liu, Mugen

2010-03-17

To identify the disease-causing gene in a four-generation Chinese family affected with retinitis pigmentosa (RP). Linkage analysis was performed with a panel of microsatellite markers flanking the candidate genetic loci of RP. These loci included 38 known RP genes. The complete coding region and exon-intron boundaries of Usher syndrome 2A (USH2A) were sequenced with the proband DNA to screen the disease-causing gene mutation. Restriction fragment length polymorphism (RFLP) analysis and direct DNA sequence analysis were done to demonstrate co-segregation of the USH2A mutations with the family disease. One hundred normal controls were used without the mutations. The disease-causing gene in this Chinese family was linked to the USH2A locus on chromosome 1q41. Direct DNA sequence analysis of USH2A identified two novel mutations in the patients: one missense mutation p.G1734R in exon 26 and a splice site mutation, IVS32+1G>A, which was found in the donor site of intron 32 of USH2A. Neither the p.G1734R nor the IVS32+1G>A mutation was found in the unaffected family members or the 100 normal controls. One patient with a homozygous mutation displayed only RP symptoms until now, while three patients with compound heterozygous mutations in the family of study showed both RP and hearing impairment. This study identified two novel mutations: p.G1734R and IVS32+1G>A of USH2A in a four-generation Chinese RP family. In this study, the heterozygous mutation and the homozygous mutation in USH2A may cause Usher syndrome Type II or RP, respectively. These two mutations expand the mutant spectrum of USH2A.

Asupan zat gizi mikro dengan fungsi kognitif pada lanjut usia

Directory of Open Access Journals (Sweden)

Ade Rahmawati

2012-04-01

Full Text Available Background: The elderly population in Indonesia increased signifi cantly in the last two decades as the life expectancy increase from 66.7 years to 70.5 years with impact to the emergence of diseases in the elderly. One of them is degradation of cognitive function that may disrupt productivity, daily activities, and quality of life of the elderly. Adequate micronutrients intake may help in maintaining cognitive function. Objectives: To identify association between intake of micronutrients (vitamin B6, folate, vitamin C, and vitamin E and cognitive function of the elderly at Among Yuswa Club of Banteng Baru, District of Sleman. Methods: This was an observational study with cross sectional design. Subjects were elderly people at Among Yuswa Club of Banteng Baru, District of Sleman. Data on cognitive function was obtained using the Mini Mental State Examination (MMSE and intake of micronutrients were collected using Semi Qualitative Food Frequency Questionnaire (SQFFQ. The data was analysed by chi square and logistic regression tests. Results: The result showed statistically signifi cant association between cognitive function and intake of vitamin B6 (p=0.010; RP=2.514; 95%CI=1.133-5.575, vitamin C intake (p=0.011; RP=3.039; 95%CI=1.454-6.353 and vitamin E intake (p=0.021; RP=1.266; 95%CI=1.057-1.516; meanwhile folate intake has no signifi cant association with cognitive function (p>0.05. Conclusion: There were signifi cant associations between intake of vitamin B6, vitamin C and vitamin E with cognitive function. There was no signifi cant association found between intake of folate and cognitive function in the elderly.

Modification of radiation therapy by RP-170, hypoxic cell sensitizer and OK-432, biological response modifier (BRM)

International Nuclear Information System (INIS)

Murayama, Chieko; Mori, Tomoyuki; Tamauchi, Hidekazu

1995-01-01

Studies have shown that the combination therapy with RP-170, irradiation and OK-432 could produce significantly higher antitumor effect. The multiple local administration of OK-432 showed superior sensitizing effect in tumor growth delay, tumor control and survival rate. DMF from TCD 50 assay was 2.09 which might be equivalent to that from irradiation with over several hundred mg/kg of RP-170. Additionally combined with 50 mg/kg RP-170, nearly available dose in clinical use, the combined treatment with RP-170, irradiation and OK-432 produced DMF of 1.4, which could be gotten by over 100 mg/kg of RP-170. The success of this combination therapy on murine tumors might also suggest a possibility for a great benefit in clinical radiotherapy. More detailed investigations, histological and immunological research, are under progress. 14 refs., 2 tabs., 3 figs

[A study of PDE6B gene mutation and phenotype in Chinese cases with retinitis pigmentosa].

Science.gov (United States)

Cui, Yun; Zhao, Kan-xing; Wang, Li; Wang, Qing; Zhang, Wei; Chen, Wei-ying; Wang, Li-ming

2003-01-01

To identify the mutation spectrum of phosphodiesterase beta subunit (PDE6B) gene, the incidence in Chinese patients with retinitis pigmentosa (RP) and their clinical phenotypic characteristics. Screening of mutations within PDE6B gene was performed using polymerase chain reaction-heteroduplex-single strand conformation polymorphism (PCR-SSCP) and DNA sequence in 35 autosomal recessive (AR) RP and 55 sporadic RP cases. The phenotypes of the patients with the gene mutation were examined and analyzed. Novel complex heterozygous variants of PDE6B gene in a sporadic case, a T to C transversion in codon 323 resulting in the substitution of Gly by Ser and 2 base pairs (bp: G and T) insert between the 27th-28th bp upstream of the 5'-end of exon 10 were both present in a same isolate RP. But they are not found in 100 unrelated healthy individuals. Ocular findings showed diffuse pigmentary retinal degeneration in the midperipheral and peripheral fundi, optic atrophy and vessel attenuation. Multi-focal ERG indicated that the rod function was more severely deteriorated. A mutation was found in a case with RP in a ARRP family, a G to A transversion at 19th base upstream 5'-end of exon 11 (within intron 10) of PDE6B gene. A sporadic RP carried a sequence variant of PDE6B gene, a G to C transition, at the 15th base adjacent to the 3'-end of exon l8. In another isolate case with RP was found 2 bp (GT) insert between 31st and 32nd base upstream 5'-end of exon 4 (in intron 3) of PDE6B gene. There are novel complex heterozygous mutations of PDE6B gene responsible for a sporadic RP patient in China. This gene mutation associated with rod deterioration and RP. Several DNA variants were found in introns of PDE6B gene in national population.

Ice Sheet Model Intercomparison Project (ISMIP6) contribution to CMIP6

NARCIS (Netherlands)

Nowicki, Sophie M J; Payne, Anthony; Larour, Eric; Seroussi, Helene; Goelzer, Heiko|info:eu-repo/dai/nl/412549123; Lipscomb, William; Gregory, Jonathan; Abe-Ouchi, Ayako; Shepherd, Andrew

2016-01-01

Reducing the uncertainty in the past, present, and future contribution of ice sheets to sea-level change requires a coordinated effort between the climate and glaciology communities. The Ice Sheet Model Intercomparison Project for CMIP6 (ISMIP6) is the primary activity within the Coupled Model

Development and Validation of a Stability-Indicating RP-HPLC ...

African Journals Online (AJOL)

Development and Validation of a Stability-Indicating RP-HPLC Method for ... of Paracetamol, Tramadol HCl and Domperidone in a Combined Dosage Form. ... testing, as well as for quality control of the combined drugs in pharmaceutical ...

Study of the RP-10 reactor neutron beam applied to the neutron radiography

International Nuclear Information System (INIS)

Zegarra, Manuel; Lopez, Alcides

2013-01-01

We have studied the RP-10 reactor radial neutron beam No. 3, which is used for neutron radiographies, by comparing radiograph's with and without the inner duct, and neutron flux determination with in flakes along the external duct, being the presence of photons creating signals at comparable levels of neutron effects, which reduce the quality of the analysis, values around 10 6 and 10 4 n/cm 2 s for thermal and epithermal flux were obtained respectively. It is recommended evaluate the design of the internal duct which presents strong photon emission. (authors).

Mass measurement on the rp-process waiting point {sup 72}Kr

Energy Technology Data Exchange (ETDEWEB)

Rodriguez, D. [Gesellschaft fuer Schwerionenforschung mbH, Darmstadt (Germany); Kolhinen, V.S. [Jyvaeskylae Univ. (Finland); Audi, G. [CSNSM-IN2P3-Centre National de la Recherche Scientifique (CNRS), 91 - Orsay (FR)] [and others

2004-06-01

The mass of one of the three major waiting points in the astrophysical rp-process {sup 72}Kr was measured for the first time with the Penning trap mass spectrometer ISOLTRAP. The measurement yielded a relative mass uncertainty of {delta}m/m=1.2 x 10{sup -7} ({delta}m=8 keV). Other Kr isotopes, also needed for astrophysical calculations, were measured with more than one order of magnitude improved accuracy. We use the ISOLTRAP masses of{sup 72-74}Kr to reanalyze the role of the {sup 72}Kr waiting point in the rp-process during X-ray bursts. (orig.)

Loss of function mutations in RP1 are responsible for retinitis pigmentosa in consanguineous familial cases

Science.gov (United States)

Kabir, Firoz; Ullah, Inayat; Ali, Shahbaz; Gottsch, Alexander D.H.; Naeem, Muhammad Asif; Assir, Muhammad Zaman; Khan, Shaheen N.; Akram, Javed; Riazuddin, Sheikh; Ayyagari, Radha; Hejtmancik, J. Fielding

2016-01-01

Purpose This study was undertaken to identify causal mutations responsible for autosomal recessive retinitis pigmentosa (arRP) in consanguineous families. Methods Large consanguineous families were ascertained from the Punjab province of Pakistan. An ophthalmic examination consisting of a fundus evaluation and electroretinography (ERG) was completed, and small aliquots of blood were collected from all participating individuals. Genomic DNA was extracted from white blood cells, and a genome-wide linkage or a locus-specific exclusion analysis was completed with polymorphic short tandem repeats (STRs). Two-point logarithm of odds (LOD) scores were calculated, and all coding exons and exon–intron boundaries of RP1 were sequenced to identify the causal mutation. Results The ophthalmic examination showed that affected individuals in all families manifest cardinal symptoms of RP. Genome-wide scans localized the disease phenotype to chromosome 8q, a region harboring RP1, a gene previously implicated in the pathogenesis of RP. Sanger sequencing identified a homozygous single base deletion in exon 4: c.3697delT (p.S1233Pfs22*), a single base substitution in intron 3: c.787+1G>A (p.I263Nfs8*), a 2 bp duplication in exon 2: c.551_552dupTA (p.Q185Yfs4*) and an 11,117 bp deletion that removes all three coding exons of RP1. These variations segregated with the disease phenotype within the respective families and were not present in ethnically matched control samples. Conclusions These results strongly suggest that these mutations in RP1 are responsible for the retinal phenotype in affected individuals of all four consanguineous families. PMID:27307693

A small potassium current in AgRP/NPY neurons regulates feeding behavior and enery metabolism

Science.gov (United States)

Neurons that co-express agouti-related peptide (AgRP) and neuropeptide Y (NPY) are indispensable for normal feeding behavior. Firing activities of AgRP/NPY neurons are dynamically regulated by energy status and coordinate appropriate feeding behavior to meet nutritional demands. However, intrinsic m...

Evaluation of operational incidents in the research reactor RP-10 according to scale INES; Evaluacion de incidentes operacionales en el reactor de investigacion RP-10 segun escala INES

Energy Technology Data Exchange (ETDEWEB)

Arrieta, Rolando W.B.; Vela Mora, Mariano, E-mail: rarrieta@ipen.gob.pe, E-mail: mvela@ipen.gob.pe [Instituto Peruano de energia Nuclear, Lima (Peru). Dept. de Operacion de Reactores

2013-07-01

This report presents the evaluation of the events in 2011 in the RP-10 Nuclear Reactor Nuclear Center Huarangal from the point of view of safety. To classify these events produced is used Scale International Nuclear and Radiological Event Scale (INES) to facilitate a common understanding between the technical community, the media and the general public. From the results we can say that in 2011 all related to security events that occurred in the RP -10 are classified as 'below scale' or no safety significance. (author)

RpA, an extracellular protease similar to the metalloprotease of serralysin family, is required for pathogenicity of Ralstonia pickettii.

Science.gov (United States)

Chen, C-M; Liu, J-J; Chou, C-W; Lai, C-H; Wu, L-T

2015-10-01

To investigate the biochemical and functional properties of an extracellular protease, RpA, in Ralstonia pickettii WP1 isolated from water supply systems. An extracellular protease was identified and characterized from R. pickettii WP1. A mutant strain WP1M2 was created from strain WP1 by mini-Tn5 transposition. The culture filtrates from WP1M2 had a lower cytotoxic effect than the parental WP1 on several mammalian cell lines. Cloning and sequence analysis revealed the Tn5 transposon inserted at a protease gene (rpA) which is 81% homologous to prtA and aprX genes of Pseudomonas fluorescens. The rpA gene encodes a 482-residue protein showing sequence similarity to metalloproteases of the serralysin family. The RpA protein was expressed in Escherichia coli using a pET expression vector and purified as a 55 kDa molecular weight protein. Furthermore, the protease activity of RpA was inhibited by protease inhibitor and heat treatment. The in vitro cytotoxic activity of R. pickettii culture filtrates was attributed to RpA protease. An extracellular protease, RpA, was identified from R. pickettii WP1 isolated from water supply system. The RpA metalloproteases is required for the pathogenicity of R. pickettii to mammalian cell lines. © 2015 The Society for Applied Microbiology.

Somato-dendritic localization and signaling by leptin receptors in hypothalamic POMC and AgRP neurons.

Directory of Open Access Journals (Sweden)

Sangdeuk Ha

Full Text Available Leptin acts via neuronal leptin receptors to control energy balance. Hypothalamic pro-opiomelanocortin (POMC and agouti-related peptide (AgRP/Neuropeptide Y (NPY/GABA neurons produce anorexigenic and orexigenic neuropeptides and neurotransmitters, and express the long signaling form of the leptin receptor (LepRb. Despite progress in the understanding of LepRb signaling and function, the sub-cellular localization of LepRb in target neurons has not been determined, primarily due to lack of sensitive anti-LepRb antibodies. Here we applied light microscopy (LM, confocal-laser scanning microscopy (CLSM, and electron microscopy (EM to investigate LepRb localization and signaling in mice expressing a HA-tagged LepRb selectively in POMC or AgRP/NPY/GABA neurons. We report that LepRb receptors exhibit a somato-dendritic expression pattern. We further show that LepRb activates STAT3 phosphorylation in neuronal fibers within several hypothalamic and hindbrain nuclei of wild-type mice and rats, and specifically in dendrites of arcuate POMC and AgRP/NPY/GABA neurons of Leprb (+/+ mice and in Leprb (db/db mice expressing HA-LepRb in a neuron specific manner. We did not find evidence of LepRb localization or STAT3-signaling in axon-fibers or nerve-terminals of POMC and AgRP/NPY/GABA neurons. Three-dimensional serial EM-reconstruction of dendritic segments from POMC and AgRP/NPY/GABA neurons indicates a high density of shaft synapses. In addition, we found that the leptin activates STAT3 signaling in proximity to synapses on POMC and AgRP/NPY/GABA dendritic shafts. Taken together, these data suggest that the signaling-form of the leptin receptor exhibits a somato-dendritic expression pattern in POMC and AgRP/NPY/GABA neurons. Dendritic LepRb signaling may therefore play an important role in leptin's central effects on energy balance, possibly through modulation of synaptic activity via post-synaptic mechanisms.

Three novel and the common Arg677Ter RP1 protein truncating mutations causing autosomal dominant retinitis pigmentosa in a Spanish population

Directory of Open Access Journals (Sweden)

Antiñolo Guillermo

2006-04-01

Full Text Available Abstract Background Retinitis pigmentosa (RP, a clinically and genetically heterogeneous group of retinal degeneration disorders affecting the photoreceptor cells, is one of the leading causes of genetic blindness. Mutations in the photoreceptor-specific gene RP1 account for 3–10% of cases of autosomal dominant RP (adRP. Most of these mutations are clustered in a 500 bp region of exon 4 of RP1. Methods Denaturing gradient gel electrophoresis (DGGE analysis and direct genomic sequencing were used to evaluate the 5' coding region of exon 4 of the RP1 gene for mutations in 150 unrelated index adRP patients. Ophthalmic and electrophysiological examination of RP patients and relatives according to pre-existing protocols were carried out. Results Three novel disease-causing mutations in RP1 were detected: Q686X, K705fsX712 and K722fsX737, predicting truncated proteins. One novel missense mutation, Thr752Met, was detected in one family but the mutation does not co-segregate in the family, thereby excluding this amino acid variation in the protein as a cause of the disease. We found the Arg677Ter mutation, previously reported in other populations, in two independent families, confirming that this mutation is also present in a Spanish population. Conclusion Most of the mutations reported in the RP1 gene associated with adRP are expected to encode mutant truncated proteins that are approximately one third or half of the size of wild type protein. Patients with mutations in RP1 showed mild RP with variability in phenotype severity. We also observed several cases of non-penetrant mutations.

Protective role of AgRP neuron's PDK1 against salt-induced hypertension.

Science.gov (United States)

Zhang, Boyang; Nakata, Masanori; Lu, Ming; Nakae, Jun; Okada, Takashi; Ogawa, Wataru; Yada, Toshihiko

2018-06-12

In the hypothalamic arcuate nucleus (ARC), orexigenic agouti-related peptide (AgRP) neurons regulate feeding behavior and energy homeostasis. The 3-phosphoinositide-dependent protein kinase-1 (PDK1) in AgRP neurons serves as a major signaling molecule for leptin and insulin, the hormones regulating feeding behavior, energy homeostasis and circulation. However, it is unclear whether PDK1 in AGRP neurons is also involved in regulation of blood pressure. This study explored it by generating and analyzing AgRP neuron-specific PDK1 knockout (Agrp-Pdk1 flox/flox ) mice and effect of high salt diet on blood pressure in KO and WT mice was analyzed. Under high salt diet feeding, systolic blood pressure (SBP) of Agrp-Pdk1 flox/flox mice was significantly elevated compared to Agrp-Cre mice. When the high salt diet was switched to control low salt diet, SBP of Agrp-Pdk1 flox/flox mice returned to the basal level observed in Agrp-Cre mice within 1 week. In Agrp-Pdk1 flox/flox mice, urinary noradrenalin excretion and NUCB2 mRNA expression in hypothalamic paraventricular nucleus (PVN) were markedly upregulated. Moreover, silencing of NUCB2 in the PVN counteracted the rises in urinary noradrenalin excretions and SBP. These results demonstrate a novel role of PDK1 in AgRP neurons to counteract the high salt diet-induced hypertension by preventing hyperactivation of PVN nesfatin-1 neurons. Copyright © 2018 Elsevier Inc. All rights reserved.

Development and validation of RP-HPLC method for analysis of multicomponent cough-cold syrup formulation

OpenAIRE

Ivković, Branka; Marković, Bojan; Vladimirov, Sote

2014-01-01

In this study a reversed phase HPLC method for rapid and simultaneous identification and quantification of doxylamine succinate, ephedrine sulfate, dextrometorphane hydrobromide, paracetamole and sodium benzoate in cough-cold syrup formulation was described. Separation was carried out on XTerraTM RP 18, Waters (150 mm x 4.6 mm column, 5 μm particle size). For the analysis of investigated substances gradient elution was used employing water, pH adjusted at 2.5 with 85 % ortophosphoric acid as ...

Development of a definition for Rapid Progression (RP) of renal function in HIV-positive persons

DEFF Research Database (Denmark)

Kamara, David A; Nielsen, Lene Ryom; Ross, Michael

2014-01-01

No consensus exists on how to define abnormally rapid deterioration in renal function (Rapid Progression, RP). We developed an operational definition of RP in HIV-positive persons with baseline estimated glomerular filtration rate (eGFR) >90ml/min/1.73m2 (using Cockcroft Gault) in the Data...

Development and Validation of a RP-HPLC Method for the ...

African Journals Online (AJOL)

Development and Validation of a RP-HPLC Method for the Simultaneous Determination of Rifampicin and a Flavonoid Glycoside - A Novel ... range, accuracy, precision, limit of detection, limit of quantification, robustness and specificity.

Formation of Escherichia coli Hfr strains by integrative suppression with the P group plasmid RP1.

OpenAIRE

Martin, R R; Thorlton, C L; Unger, L

1981-01-01

Hfr strains of Escherichia coli were obtained by integrative suppression of a dnaA(Ts) mutation by the Inc P-1 plasmid RP1 without prior creation of an unnatural homology between the plasmid and the E. coli chromosome. Unmodified RP1 mobilized the polarized transfer of the chromosome in a counterclock-wise direction from a distinct origin between 81 min (pyrE) and 82 min (dnaA) with pyrE as a leading marker. Inheritance of RP1-Hfr chromosomal and antibiotic resistance genes was due to recombi...

A novel electrophototrophic bacterium Rhodopseudomonas palustris strain RP2, exhibits hydrocarbonoclastic potential in anaerobic environments

Directory of Open Access Journals (Sweden)

Krishnaveni Venkidusamy

2016-07-01

Full Text Available An electrophototrophic, hydrocarbonoclastic bacterium Rhodopseudomonas palustris stain RP2 was isolated from the anodic biofilms of hydrocarbon fed microbial electrochemical remediation systems (MERS. Salient properties of the strain RP2 were direct electrode respiration, dissimilatory metal oxide reduction, spore formation, anaerobic nitrate reduction, free living diazotrophy and the ability to degrade n-alkane components of petroleum hydrocarbons in anoxic, photic environments. In acetate fed microbial electrochemical cells, a maximum current density of 305±10 mA/m2 (1000Ω was generated (power density 131.65±10 mW/m2 by strain RP2 with a coulombic efficiency of 46.7 ± 1.3%. Cyclic voltammetry studies showed that anaerobically grown cells of strain RP2 is electrochemically active and likely to transfer electrons extracellularly to solid electron acceptors through membrane bound compounds, however, aerobically grown cells lacked the electrochemical activity. The ability of strain RP2 to produce current (maximum current density 21±3 mA/m2; power density 720±7 µW/m2, 1000Ω using petroleum hydrocarbon (PH as a sole energy source was also examined using an initial concentration of 800 mg l-1 of diesel range hydrocarbons (C9- C36 with a concomitant removal of 47.4 ± 2.7% hydrocarbons in MERS. Here, we also report the first study that shows an initial evidence for the existence of a hydrocarbonoclastic behavior in the strain RP2 when grown in different electron accepting and illuminated conditions (anaerobic and MERS degradation. Such observations reveal the importance of photoorganotrophic growth in the utilization of hydrocarbons from contaminated environments. Identification of such novel petrochemical hydrocarbon degrading electricigens, not only expands the knowledge on the range of bacteria known for the hydrocarbon bioremediation but also shows a biotechnological potential that goes well beyond its applications to MERS.

PAP-1, the mutated gene underlying the RP9 form of dominant retinitis pigmentosa, is a splicing factor

International Nuclear Information System (INIS)

Maita, Hiroshi; Kitaura, Hirotake; Keen, T. Jeffrey; Inglehearn, Chris F.; Ariga, Hiroyoshi; Iguchi-Ariga, Sanae M.M.

2004-01-01

PAP-1 is an in vitro phosphorylation target of the Pim-1 oncogene. Although PAP-1 binds to Pim-1, it is not a substrate for phosphorylation by Pim-1 in vivo. PAP-1 has recently been implicated as the defective gene in RP9, one type of autosomal dominant retinitis pigmentosa (adRP). However, RP9 is a rare disease and only two missense mutations have been described, so the report of a link between PAP-1 and RP9 was tentative. The precise cellular role of PAP-1 was also unknown at that time. We now report that PAP-1 localizes in nuclear speckles containing the splicing factor SC35 and interacts directly with another splicing factor, U2AF35. Furthermore, we used in vitro and in vivo splicing assays to show that PAP-1 has an activity, which alters the pattern of pre-mRNA splicing and that this activity is dependent on the phosphorylation state of PAP-1. We used the same splicing assay to examine the activities of two mutant forms of PAP-1 found in RP9 patients. The results showed that while one of the mutations, H137L, had no effect on splicing activity compared with that of wild-type PAP-1, the other, D170G, resulted in both a defect in splicing activity and a decreased proportion of phosphorylated PAP-1. The D170G mutation may therefore cause RP by altering splicing of retinal genes through a decrease in PAP-1 phosphorylation. These results demonstrate that PAP-1 has a role in pre-mRNA splicing and, given that three other splicing factors have been implicated in adRP, this finding provides compelling further evidence that PAP-1 is indeed the RP9 gene

The retinitis pigmentosa-mutated RP2 protein exhibits exonuclease activity and translocates to the nucleus in response to DNA damage

International Nuclear Information System (INIS)

Yoon, Jung-Hoon; Qiu Junzhuan; Cai Sheng; Chen Yuan; Cheetham, Michael E.; Shen Binghui; Pfeifer, Gerd P.

2006-01-01

Retinitis pigmentosa (RP) is a genetically heterogeneous disease characterized by degeneration of the retina. Mutations in the RP2 gene are linked to the second most frequent form of X-linked retinitis pigmentosa. RP2 is a plasma membrane-associated protein of unknown function. The N-terminal domain of RP2 shares amino acid sequence similarity to the tubulin-specific chaperone protein co-factor C. The C-terminus consists of a domain with similarity to nucleoside diphosphate kinases (NDKs). Human NDK1, in addition to its role in providing nucleoside triphosphates, has recently been described as a 3' to 5' exonuclease. Here, we show that RP2 is a DNA-binding protein that exhibits exonuclease activity, with a preference for single-stranded or nicked DNA substrates that occur as intermediates of base excision repair pathways. Furthermore, we show that RP2 undergoes re-localization into the nucleus upon treatment of cells with DNA damaging agents inducing oxidative stress, most notably solar simulated light and UVA radiation. The data suggest that RP2 may have previously unrecognized roles as a DNA damage response factor and 3' to 5' exonuclease

75 FR 65366 - Recovery Policy RP9524.2, Landslides and Slope Stability Related to Public Facilities

Science.gov (United States)

2010-10-22

...] Recovery Policy RP9524.2, Landslides and Slope Stability Related to Public Facilities AGENCY: Federal... the final Recovery Policy RP9524.2, Landslides and Slope Stability Related to Public Facilities, which... facilities threatened by landslides or slope failures; as well as the eligibility of permanent repairs to...

75 FR 29569 - Recovery Policy RP9526.1, Hazard Mitigation Funding Under Section 406 (Stafford Act)

Science.gov (United States)

2010-05-26

...] Recovery Policy RP9526.1, Hazard Mitigation Funding Under Section 406 (Stafford Act) AGENCY: Federal... the final Recovery Policy RP9526.1, Hazard Mitigation Funding Under Section 406 (Stafford Act), which... mitigation discretionary funding available under Section 406 of the Robert T. Stafford Disaster Relief and...

The Relevance of AgRP Neuron-Derived GABA Inputs to POMC Neurons Differs for Spontaneous and Evoked Release

OpenAIRE

Rau, Andrew R.; Hentges, Shane T.

2017-01-01

Hypothalamic agouti-related peptide (AgRP) neurons potently stimulate food intake, whereas proopiomelanocortin (POMC) neurons inhibit feeding. Whether AgRP neurons exert their orexigenic actions, at least in part, by inhibiting anorexigenic POMC neurons remains unclear. Here, the connectivity between GABA-releasing AgRP neurons and POMC neurons was examined in brain slices from male and female mice. GABA-mediated spontaneous IPSCs (sIPSCs) in POMC neurons were unaffected by disturbing GABA re...

Positive inotropic effects of RP 62719, a new pure class III antiarrhythmic agent, on guinea pig myocardium.

Science.gov (United States)

Beregi, J P; Escande, D; Coudray, N; Mery, P; Mestre, M; Chemla, D; Lecarpentier, Y

1992-12-01

The mechanical effects of RP 62719 [(-)1-[-2-(3,4-dihydro-2H-1- benzopyran-4-yl)ethyl]-4-(3,4-dimethoxyphenyl)-piperidine] were tested in vitro on guinea pig left ventricular papillary muscle. RP 62719 is a novel pure class III antiarrhythmic agent known to prolong the cardiac action potential duration by selectively blocking the inward rectifying K+ current. Mechanical parameters were determined from contraction and relaxation phases under isotonic and isometric conditions. At a concentration of 0.02 microM, RP 62719 did not produce significant effects on inotropy or lusitropy. At 0.2 and 2 microM, the drug improved contraction under both heavy and low loading conditions, as evidenced by a 30% increase in maximum unloaded shortening velocity (Vmax, P delta L, P force normalized per cross-sectional area (AF/s, P force derivative per mm2 (+dF/s, P force derivative per mm2 (-dF/s, P < .001). At a higher concentration (20 microM), effects of RP 62719 on inotropy and lusitropy were less marked, thus accounting for the bell-shaped form of the dose-response curve. An increase in the extracellular Ca++ concentration from 2.5 to 3.75 mM improved inotropy to a similar extent (+30-50%) as did 2 microM RP 62719. However, lusitropy and mechanical coupling between contraction and relaxation were not modified in the same proportion under RP 62719 and under 3.75 mM Ca++.(ABSTRACT TRUNCATED AT 250 WORDS)

Haloperidol Regulates the State of Phosphorylation of Ribosomal Protein S6 via Activation of PKA and Phosphorylation of DARPP-32

Science.gov (United States)

Valjent, Emmanuel; Bertran-Gonzalez, Jesus; Bowling, Heather; Lopez, Sébastien; Santini, Emanuela; Matamales, Miriam; Bonito-Oliva, Alessandra; Hervé, Denis; Hoeffer, Charles; Klann, Eric; Girault, Jean-Antoine; Fisone, Gilberto

2011-01-01

Administration of typical antipsychotic drugs, such as haloperidol, promotes cAMP-dependent signaling in the medium spiny neurons (MSNs) of the striatum. In this study, we have examined the effect of haloperidol on the state of phosphorylation of the ribosomal protein S6 (rpS6), a component of the small 40S ribosomal subunit. We found that haloperidol increases the phosphorylation of rpS6 at the dual site Ser235/236, which is involved in the regulation of mRNA translation. This effect was exerted in the MSNs of the indirect pathway, which express specifically dopamine D2 receptors (D2Rs) and adenosine A2 receptors (A2ARs). The effect of haloperidol was decreased by blockade of A2ARs or by genetic attenuation of the Gαolf protein, which couples A2ARs to activation of adenylyl cyclase. Moreover, stimulation of cAMP-dependent protein kinase A (PKA) increased Ser235/236 phosphorylation in cultured striatal neurons. The ability of haloperidol to promote rpS6 phosphorylation was abolished in knock-in mice deficient for PKA activation of the protein phosphatase-1 inhibitor, dopamine- and cAMP-regulated phosphoprotein of 32 kDa. In contrast, pharmacological or genetic inactivation of p70 rpS6 kinase 1, or extracellular signal-regulated kinases did not affect haloperidol-induced rpS6 phosphorylation. These results identify PKA as a major rpS6 kinase in neuronal cells and suggest that regulation of protein synthesis through rpS6 may be a potential target of antipsychotic drugs. PMID:21814187

AMPK is essential for energy homeostasis regulation and glucose sensing by POMC and AgRP neurons.

Science.gov (United States)

Claret, Marc; Smith, Mark A; Batterham, Rachel L; Selman, Colin; Choudhury, Agharul I; Fryer, Lee G D; Clements, Melanie; Al-Qassab, Hind; Heffron, Helen; Xu, Allison W; Speakman, John R; Barsh, Gregory S; Viollet, Benoit; Vaulont, Sophie; Ashford, Michael L J; Carling, David; Withers, Dominic J

2007-08-01

Hypothalamic AMP-activated protein kinase (AMPK) has been suggested to act as a key sensing mechanism, responding to hormones and nutrients in the regulation of energy homeostasis. However, the precise neuronal populations and cellular mechanisms involved are unclear. The effects of long-term manipulation of hypothalamic AMPK on energy balance are also unknown. To directly address such issues, we generated POMC alpha 2KO and AgRP alpha 2KO mice lacking AMPK alpha2 in proopiomelanocortin- (POMC-) and agouti-related protein-expressing (AgRP-expressing) neurons, key regulators of energy homeostasis. POMC alpha 2KO mice developed obesity due to reduced energy expenditure and dysregulated food intake but remained sensitive to leptin. In contrast, AgRP alpha 2KO mice developed an age-dependent lean phenotype with increased sensitivity to a melanocortin agonist. Electrophysiological studies in AMPK alpha2-deficient POMC or AgRP neurons revealed normal leptin or insulin action but absent responses to alterations in extracellular glucose levels, showing that glucose-sensing signaling mechanisms in these neurons are distinct from those pathways utilized by leptin or insulin. Taken together with the divergent phenotypes of POMC alpha 2KO and AgRP alpha 2KO mice, our findings suggest that while AMPK plays a key role in hypothalamic function, it does not act as a general sensor and integrator of energy homeostasis in the mediobasal hypothalamus.

Transduced PEP-1-ribosomal protein S3 (rpS3) ameliorates 12-O-tetradecanoylphorbol-13-acetate-induced inflammation in mice

International Nuclear Information System (INIS)

Ahn, Eun Hee; Kim, Dae Won; Kang, Hye Won; Shin, Min Jae; Won, Moo Ho; Kim, Joon; Kim, Dong Joon; Kwon, Oh-Shin; Kang, Tae-Cheon; Han, Kyu Hyung; Park, Jinseu; Eum, Won Sik; Choi, Soo Young

2010-01-01

This study investigated the preventive effect of ribosomal protein S3 (rpS3) on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced ear edema in mice. A cell permeable expression vector PEP-1-rpS3 was constructed. Topical application of the vector markedly inhibited TPA-induced expression levels of cyclooxygenase-2 (COX-2) and pro-inflammatory cytokines. Application of PEP-1-rpS3 also resulted in a significant reduction in the activation of nuclear factor-kappa B (NF-kB) and mitogen-activated protein kinase (MAPK) in TPA-treated ears. These results indicate that PEP-1-rpS3 inhibits inflammatory response cytokines and enzymes by blocking NF-kB and MAPK, prompting the suggestion that PEP-1-rpS3 can be used as a therapeutic agent against skin inflammation.

Accounting for inertia in modal choices: some new evidence using a RP/SP dataset

DEFF Research Database (Denmark)

Cherchi, Elisabetta; Manca, Francesco

2011-01-01

effect is stable along the SP experiments. Inertia has been studied more extensively with panel datasets, but few investigations have used RP/SP datasets. In this paper we extend previous work in several ways. We test and compare several ways of measuring inertia, including measures that have been...... proposed for both short and long RP panel datasets. We also explore new measures of inertia to test for the effect of “learning” (in the sense of acquiring experience or getting more familiar with) along the SP experiment and we disentangle this effect from the pure inertia effect. A mixed logit model...... is used that allows us to account for both systematic and random taste variations in the inertia effect and for correlations among RP and SP observations. Finally we explore the relation between the utility specification (especially in the SP dataset) and the role of inertia in explaining current choices....

Phosphorylation of Ribosomal Protein S6 Mediates Mammalian Target of Rapamycin Complex 1-Induced Parathyroid Cell Proliferation in Secondary Hyperparathyroidism.

Science.gov (United States)

Volovelsky, Oded; Cohen, Gili; Kenig, Ariel; Wasserman, Gilad; Dreazen, Avigail; Meyuhas, Oded; Silver, Justin; Naveh-Many, Tally

2016-04-01

Secondary hyperparathyroidism is characterized by increased serum parathyroid hormone (PTH) level and parathyroid cell proliferation. However, the molecular pathways mediating the increased parathyroid cell proliferation remain undefined. Here, we found that the mTOR pathway was activated in the parathyroid of rats with secondary hyperparathyroidism induced by either chronic hypocalcemia or uremia, which was measured by increased phosphorylation of ribosomal protein S6 (rpS6), a downstream target of the mTOR pathway. This activation correlated with increased parathyroid cell proliferation. Inhibition of mTOR complex 1 by rapamycin decreased or prevented parathyroid cell proliferation in secondary hyperparathyroidism rats and in vitro in uremic rat parathyroid glands in organ culture. Knockin rpS6(p-/-) mice, in which rpS6 cannot be phosphorylated because of substitution of all five phosphorylatable serines with alanines, had impaired PTH secretion after experimental uremia- or folic acid-induced AKI. Uremic rpS6(p-/-) mice had no increase in parathyroid cell proliferation compared with a marked increase in uremic wild-type mice. These results underscore the importance of mTOR activation and rpS6 phosphorylation for the pathogenesis of secondary hyperparathyroidism and indicate that mTORC1 is a significant regulator of parathyroid cell proliferation through rpS6. Copyright © 2016 by the American Society of Nephrology.

Nuclear structure and weak rates of heavy waiting point nuclei under rp-process conditions

Science.gov (United States)

Nabi, Jameel-Un; Böyükata, Mahmut

2017-01-01

The structure and the weak interaction mediated rates of the heavy waiting point (WP) nuclei 80Zr, 84Mo, 88Ru, 92Pd and 96Cd along N = Z line were studied within the interacting boson model-1 (IBM-1) and the proton-neutron quasi-particle random phase approximation (pn-QRPA). The energy levels of the N = Z WP nuclei were calculated by fitting the essential parameters of IBM-1 Hamiltonian and their geometric shapes were predicted by plotting potential energy surfaces (PESs). Half-lives, continuum electron capture rates, positron decay rates, electron capture cross sections of WP nuclei, energy rates of β-delayed protons and their emission probabilities were later calculated using the pn-QRPA. The calculated Gamow-Teller strength distributions were compared with previous calculation. We present positron decay and continuum electron capture rates on these WP nuclei under rp-process conditions using the same model. For the rp-process conditions, the calculated total weak rates are twice the Skyrme HF+BCS+QRPA rates for 80Zr. For remaining nuclei the two calculations compare well. The electron capture rates are significant and compete well with the corresponding positron decay rates under rp-process conditions. The finding of the present study supports that electron capture rates form an integral part of the weak rates under rp-process conditions and has an important role for the nuclear model calculations.

Impact of Nuclear Power and Desalination Plant Construction Toward National and East Java Economic

International Nuclear Information System (INIS)

Ratya-Anindita; Sriyana; M-Nasrullah

2006-01-01

The objective of this study is to determine the economic impacts of the construction of the nuclear power plant 2 x 100 MW(e) SMART type with desalination 4 x 10,000 m 3 which would conduct in years 2008 to 2017 in Madura Island, East Java. The predicted IO tables of 2008-2017 have been created by the application of dynamic IO projection. The economic impact was estimated through multiplier effect which covers direct impact and indirect impact as well as the induced effect. The expenditures of SMART nuclear power and desalination plant to the domestic contractors is estimated to amount to 88.2 million US dollar or 25.6 % of the whole expenditures. The total impact of the project to the national economy would be Rp. 6,329,347 million, Rp. 8,439,130 million, and Rp. 12,658,695 million for each scenario of the exchange rate as high as Rp. 7,500/US dollar, Rp. 10,000/US dollar, Rp. 15,000/US dollar, respectively for the scenario of dynamic growth. The total impact of the project to the provincial economy of East Java would be as much as Rp. 3,253,498 million, Rp. 4,337,997 million, and Rp. 6,506,995 million for each scenario of the exchange rate as high as Rp. 7,500/US dollar, Rp. 10,000/US dollar, Rp. 15,000/US dollar, respectively under the former scenario. Cumulative direct impact since pre-construction to construction period had been calculated as much as US dollar 101.8 million for sectors number 48.52 and 62. This have brought much impact on other sectors in national or provincial levels of economy. (author)

Weak interaction rates for Kr and Sr waiting-point nuclei under rp-process conditions

International Nuclear Information System (INIS)

Sarriguren, P.

2009-01-01

Weak interaction rates are studied in neutron deficient Kr and Sr waiting-point isotopes in ranges of densities and temperatures relevant for the rp process. The nuclear structure is described within a microscopic model (deformed QRPA) that reproduces not only the half-lives but also the Gamow-Teller strength distributions recently measured. The various sensitivities of the decay rates to both density and temperature are discussed. Continuum electron capture is shown to contribute significantly to the weak rates at rp-process conditions.

Development and Validation of a RP-HPLC Method for the ...

African Journals Online (AJOL)

Erah

Methods: Reverse phase (RP) chromatographic separation and estimation was achieved ... Recently, the use of natural products in ... one of the major consequences of herb-drug ... assurance and routine analysis of the blend. .... The robustness of the method was evaluated .... compliance with regulatory requirements) of.

Rare itemsets mining algorithm based on RP-Tree and spark framework

Science.gov (United States)

Liu, Sainan; Pan, Haoan

2018-05-01

For the issues of the rare itemsets mining in big data, this paper proposed a rare itemsets mining algorithm based on RP-Tree and Spark framework. Firstly, it arranged the data vertically according to the transaction identifier, in order to solve the defects of scan the entire data set, the vertical datasets are divided into frequent vertical datasets and rare vertical datasets. Then, it adopted the RP-Tree algorithm to construct the frequent pattern tree that contains rare items and generate rare 1-itemsets. After that, it calculated the support of the itemsets by scanning the two vertical data sets, finally, it used the iterative process to generate rare itemsets. The experimental show that the algorithm can effectively excavate rare itemsets and have great superiority in execution time.

REVIEW ON CONSTRUCTION PROJECT MANAGEMENT SOFTWARE PRIMAVERA P6

OpenAIRE

Piyush Pramod Bagade* & Prof. Abhijit Bhirud

2018-01-01

Planning, scheduling and Resource levelling plays an important role in any construction project maybe it is construction of building or construction of road. In absence of proper planning, scheduling and resource levelling construction industry does not get profit over the project. For this purpose, proper software’s and techniques must be utilize. This paper is focussing on the advantages of Oracle Primavera P6 Software. The latest version of Primavera is P6-17. In any construction work huge...

Evaluation of operational incidents in the research reactor RP-10 according to scale INES

International Nuclear Information System (INIS)

Arrieta, Rolando W.B.; Vela Mora, Mariano

2013-01-01

This report presents the evaluation of the events in 2011 in the RP-10 Nuclear Reactor Nuclear Center Huarangal from the point of view of safety. To classify these events produced is used Scale International Nuclear and Radiological Event Scale (INES) to facilitate a common understanding between the technical community, the media and the general public. From the results we can say that in 2011 all related to security events that occurred in the RP -10 are classified as 'below scale' or no safety significance. (author)

Prescreening based on the presence of CT-scan abnormalities and biomarkers (KL-6 and SP-D may reduce severe radiation pneumonitis after stereotactic radiotherapy

Directory of Open Access Journals (Sweden)

Wakui Reiko

2010-05-01

Full Text Available Abstract Purpose To determine the risk factors of severe radiation pneumonitis (RP after stereotactic body radiation therapy (SBRT for primary or secondary lung tumors. Materials and methods From January 2003 to March 2009, SBRT was performed on 117 patients (32 patients before 2005 and 85 patients after 2006 with lung tumors (primary = 74 patients and metastatic/recurrent = 43 patients in our institution. In the current study, the results on cases with severe RP (grades 4-5 were evaluated. Serum Krebs von den Lungen-6 (KL-6 and serum Surfactant protein-D (SP-D were used to predict the incidence of RP. A shadow of interstitial pneumonitis (IP on the CT image before performing SBRT was also used as an indicator for RP. Since 2006, patients have been prescreened for biological markers (KL-6 & SP-D as well as checking for an IP-shadow in CT. Results Grades 4-5 RP was observed in nine patients (7.7% after SBRT and seven of these cases (6.0% were grade 5 in our institution. A correlation was found between the incidence of RP and higher serum KL-6 & SP-D levels. IP-shadow in patient's CT was also found to correlate well with the severe RP. Severe RP was reduced from 18.8% before 2005 to 3.5% after 2006 (p = 0.042. There was no correlation between the dose volume histogram parameters and these severe RP patients. Conclusion Patients presenting with an IP shadow in the CT and a high value of the serum KL-6 & SP-D before SBRT treatment developed severe radiation pneumonitis at a high rate. The reduction of RP incidence in patients treated after 2006 may have been attributed to prescreening of the patients. Therefore, pre-screening before SBRT for an IP shadow in CT and serum KL-6 & SP-D is recommended in the management and treatment of patients with primary or secondary lung tumors.

Improved soil characterization for pipe piles in sand in API RP-2A

International Nuclear Information System (INIS)

Hossain, M.K.; Briaud, J.L.

1993-01-01

In the offshore, most foundations are steel pipe piles and most of them are designed using the API RP 2A guidelines. For axial capacity of piles in sand the current guidelines in many cases show definite discrepancies when compared against actual load capacities of piles. An updated data base analysis shows that there are three major weaknesses in the current guidelines with respect to soil characterization: (a) the consideration of the lateral earth pressure coefficient, K, as a constant (1.0 or 0.8); (b) the consideration of the unit point bearing resistance, q, as a linear function of depth; and (c) the absence of an unambiguous soil parameter determination process based on reliable in-situ test results. In this paper, specific modifications to the current API RP 2A guidelines are proposed on the basis of a data base analysis to account for the discrepancies arising from (a), (b), and (c) above. These modifications will reduce the discrepancies in the current API RP 2A method and increase the accuracy of the prediction of axial capacity of pipe piles in sand. Furthermore this will make the method fundamentally more consistent with soil behavior in deep foundations

Numerical Investigation on Supercritical Heat Transfer of RP3 Kerosene Flowing inside a Cooling Channel of Scramjet

Directory of Open Access Journals (Sweden)

Ning Wang

2014-06-01

Full Text Available Supercritical convective heat transfer characteristics of hydrocarbon fuel play a fundamental role in the active cooling technology of scramjet. In this paper, a 2D-axisymmetric numerical study of supercritical heat transfer of RP3 flowing inside the cooling channels of scramjet has been conducted. The main thermophysical properties of RP3, including density, specific heat, and thermal conductivity, are obtained from experimental data, while viscosity is evaluated from a commercial code with a ten-species surrogate. Effects of heat flux, mass flow rate, and inlet temperature on supercritical heat transfer processes have been investigated. Results indicate that when the wall temperature rises above the pseudocritical temperature of RP3, heat transfer coefficient decreases as a result of drastic decrease of the specific heat. The conventional heat transfer correlations, that is, Gnielinski formula, are no longer proper for the supercritical heat transfer of RP3. The modified Jackson and Hall formula, which was proposed for supercritical CO2 and water, gives good prediction except when the wall temperature is near or higher than the pseudocritical temperature.

GEO-6 project for Galileo data scientific utilization

Science.gov (United States)

Buresova, Dalia; Lastovicka, Jan; Boska, Josef; Sauli, Petra; Kouba, Daniel; Mosna, Zbysek

The future GNSS Galileo system offer a number of benefits (e.g. availability of better accuracy positioning, new frequencies bands allowing the implementation of specific techniques, provable time-stamp and location data using SIS authorisation, integrity, better support ad-hoc algorithms for data analysis and other service guarantee for liability and regulated applications) are widely spread among different disciplines. Also applications which are less interesting from the commercial and market point of view could successfully contribute to the numerous social benefits and support the innovation in the international research. The aim of the GEO-6 project "Scientific research Using GNSS" is to propose and broaden scientific utilization of future GNSS Galileo system data in research. It is a joint project of seven institutions from six countries led by the Atos Origin Company from Spain. The core of the project consists from six projects in five priority areas: PA-1 Remote sensing of the ocean using GNSS reflections, PA-2a Investigating GNSS ionospheric data assimilation, PA-2b 3-D gravity wave detection and determination (both PA-2a and PA-2b are ionospheric topics), PA-3 Demonstration of capability for operational forecasting of atmospheric delays, PA-4 GNSS seismometer, PA-5 Spacecraft formation flying using global navigation satellite systems. Institute of Atmospheric Physics, Prague, Czech Republic is responsible for the project PA-2b, where we developed and tested (to the extent allowed by available data) an algorithm and computer code for the 3-D detection of gravity waves and determination of their characteristics. The main drivers of the GEO-6 project are: high levels of accuracy even with the support of local elements, sharing of solutions and results for the worldwide scientific community. The paper will present basic description of the project with more details concerning Czech participation in it.

75 FR 24740 - Recovery Fact Sheet RP9580.102, Permanent Relocation

Science.gov (United States)

2010-05-05

... DEPARTMENT OF HOMELAND SECURITY Federal Emergency Management Agency [Docket ID FEMA-2010-0028] Recovery Fact Sheet RP9580.102, Permanent Relocation AGENCY: Federal Emergency Management Agency, DHS... Management Agency (FEMA). DATES: This fact sheet is effective April 14, 2010. ADDRESSES: This final fact...

Characterization and adjustment of the neutron radiography facility of the RP-10 nuclear reactor; Caracterizacion y puesta a punto de la facilidad de neutrografia del reactor nuclear RP-10

Energy Technology Data Exchange (ETDEWEB)

Ravello R, Y R

2001-07-01

The main aim of this work was to characterize and adjust the neutron radiography facility of the RP-10 nuclear reactor, and therefore be able to offer with this technique services to the industry and research centers in general. This technique will be complemented with others such as x-rays and gamma radiography. First, the shielding capacity of the facility was analyzed, proving that it complies with the radiological safety requirements established by the radiological safety code. Then gamma filtration tests were conducted in order to implement the direct method for image formation, optical density curves were built according to the thickness of the gamma filter, the type of film and the type of irradiation. Also, the indirect method for image formation was implemented for two types of converters: indium and dysprosium. Growth curves for optical density were also made according to contact time between converter-film, for different types of films. The resolution of the facility was also analyzed using two methods: Klasens (1946) and Harms (1986). Harms method came closer to the resolution of the human eye when compared to the Klasens method. Finally, the application fields of neutron radiography are presented, including those conducted at the neutron radiography facility of the RP-10 nuclear reactor. With this work, the RP-10 neutron radiography facility is ready to offer inspection and research services.

Numerical study of the enlarged O(5) symmetry of the 3D antiferromagnetic RP2 spin model

International Nuclear Information System (INIS)

Fernandez, L.A.; Martin-Mayor, V.; Sciretti, D.; Tarancon, A.; Velasco, J.L.

2005-01-01

We investigate by means of Monte Carlo simulation and finite-size scaling analysis the critical properties of the three-dimensional O(5) non-linear σ model and of the antiferromagnetic RP 2 model, both of them regularized on a lattice. High accuracy estimates are obtained for the critical exponents, universal dimensionless quantities and critical couplings. It is concluded that both models belong to the same universality class, provided that rather non-standard identifications are made for the momentum-space propagator of the RP 2 model. We have also investigated the phase diagram of the RP 2 model extended by a second-neighbor interaction. A rich phase diagram is found, where most of the phase transitions are of the first order

Resonance parameter and covariance evaluation for 16O up to 6 MeV

Directory of Open Access Journals (Sweden)

Leal Luiz

2016-01-01

Full Text Available A resolved resonance evaluation was performed for 16O in the energy range 0 eV to 6 MeV using the computer code SAMMY resulting in a set of resonance parameters (RPs that describes well the experimental data used in the evaluation. A RP covariance matrix (RPC was also generated. The RP were converted to the evaluated nuclear data file format using the R-Matrix Limited format and the compact format was used to represent the RPC. In contrast to the customary use of RP, which are frequently intended for the generation of total, capture, and scattering cross sections only, the present RP evaluation permits the computation of angle dependent cross sections. Furthermore, the RPs are capable of representing the (n, α cross section from the energy threshold (2.354 MeV of the (n, α reaction to 6 MeV. The intent of this paper is to describe the procedures used in the evaluation of the RP and RPC, the use of the RPC in benchmark calculations and to assess the impact of the 16O nuclear data uncertainties in the calculate dkeff for critical benchmark experiments.

[Determination of aristolochic acid A in Guanxinsuhe preparations by RP-HPLC].

Science.gov (United States)

Li, Lin; Gao, Hui-Min; Wang, Zhi-Min; Wang, Wei-Hao

2006-01-01

To establish a determination method of aristolochic acid A in Guanxisuhe preparations by RP-HPLC. The instrument used was Hewlett-Packard 1100 HPLC with a Alltech C18 column (4.6 mm x 250 mm, 5 microm). The mobile phase was methanol-water-acetic acid (68: 32:1) and the flow rate was 1.0 mL x min(-1). The UV detection wavelength was 390 nm and the column temperature was at 35 degrees C. The extracted solvent for the preparations was methanol solution contained 10% formic acid. The calibration curve was linear (r = 0.999 9) within the range of 0.119-1.89 microg for aristolochic acid A. The average recovery 99.0%, RSD 0.63%. The method with good linear relationship was convenient, quick, accurate, and suitable for the quality control of the aristolochic acid A in Guanxinsuhe and other traditional Chinese medicines containing aristolochic acid A.

Paleo-Productivity across the Paleocene-Eocene Thermal Maximum, Walvis Ridge Transect (ODP Sites 1262, 1263, and 1266)

Science.gov (United States)

Chun, C. O.; Delaney, M. L.; Zachos, J. C.

2005-12-01

Walvis Ridge transect (Ocean Drilling Program (ODP) Leg 208) provides the first high-resolution depth-transect of deep-sea sediments recovered from the south Atlantic across the P/E boundary. A geographically restricted depth transect (~ 2.2 km, water depths between 2500 and 4770 m) allows us to constrain the surface waters by assuming marine productivity conditions in the overlying water column are similar across all sites. The sediment record will reveal variations for processes that are water-depth dependent. We use the geochemical tracers; biogenic barium, phosphorus, calcium carbonate, and the redox sensitive trace elements manganese and uranium, to reconstruct nutrient burial, paleoproductivity, and bottom water redox chemistry across the Paleocene-Eocene Thermal Maximum (PETM). We calculate our concentrations on a calcium carbonate-free basis to account for dilution by non-carbonate sediments. Trace metal enrichment factors (EFs) are calculated relative to bulk crustal averages. We chose three sites from the depth transect: the shallowest (Site 1263, 2717 m water depth), an intermediate site (Site 1266, 3798 m water depth), and the deepest site (Site 1262, 4755 m water depth). We sampled each site at a sample resolution of ~ 1-2 kyr for 5 m.y. centered at 55 Ma. Uranium EFs at the shallow site exhibits values ~ 5 pre-event and drop to values near crustal averages during and after the carbon isotope excursion (CIE). No dramatic changes in U EFs across the P/E boundary are recorded at the deep and intermediate sites. Mn EFs range between 2.9 -8.6 prior to the event across all three sites, suggesting an oxygenated depositional environment. At the boundary, Mn EFs drop to crustal averages at all sites, then gradually return to pre-event values, indicating more reducing environments during the CIE, a possible explanation for the benthic extinction event (BEE) observed across this transect. Ba excess and reactive phosphorus exhibit decreased concentrations during

WSSRAP chemical plant geotechnical investigations for the Weldon Spring Site Remedial Action Project, Weldon Spring, Missouri

International Nuclear Information System (INIS)

1990-12-01

This document has been prepared for the United states Department of Energy (DOE) Weldon Spring Site Remedial Action Project (WSSRAP) by the Project Management Contractor (PMC), which consists of MK-Ferguson Company (MKF) and Morrison Knudsen Corporation Environmental Services Group (MKES) with Jacobs Engineering Group (JEG) as MKF's predesignated subcontractor. This report presents the results of site geotechnical investigations conducted by the PMC in the vicinity of the Weldon Spring chemical plant and raffinate pits (WSCP/RP) and in potential on-site and off-site clayey material borrow sources. The WSCP/RP is the proposed disposal cell (DC) site. 39 refs., 24 figs., 12 tabs

Twenty years of Radiology in RP-10 nuclear reactor protection; Veinte anos de proteccion radiologica en el reactor nuclear RP-10

Energy Technology Data Exchange (ETDEWEB)

Zapata, Alejandro L.; Ramos, Fernando T.; Arrieta, Rolando W.B.; Vela Mora, Mariano, E-mail: lzapata@ipen.gob.pe, E-mail: framos@ipen.gob.pe, E-mail: rarrieta@ipen.gob.pe, E-mail: mvela@ipen.gob.pe [Instituto Peruano de Energia Nuclear (IPEN), Lima (Peru)

2013-07-01

In this report we present the results about radiation controls during 1990 - 2010, carried out in the Nuclear Reactor RP-10 of the Nuclear Center of Huarangal. These controls and radiological evaluation are of much utility for the radio personnel protection of this one and other reactors, since it allows to compares these variables with respect to the time. From the results obtained in monitoring and radiation controls, we conclude that in no case it has been reached the limits allowed by the Peruvian Regulating Authority. (author)

Witnessing the first sign of retinitis pigmentosa onset in the allegedly normal eye of a case of unilateral RP: a 30-year follow-up.

Science.gov (United States)

Gauvin, Mathieu; Chakor, Hadi; Koenekoop, Robert K; Little, John M; Lina, Jean-Marc; Lachapelle, Pierre

2016-06-01

A patient initially presented with constricted visual field, attenuated retinal vasculature, pigmentary clumping and reduced ERG in OS only, suggestive of unilateral retinitis pigmentosa (RP). This patient was subsequently seen on eight occasions (over three decades), and, with time, the initially normal eye (OD) gradually showed signs of RP-like degeneration. The purpose of this study was to evaluate which clinical modality (visual field, funduscopy or electroretinography) could have first predicted this fate. At each time points, data obtained from our patient were compared to normative data using Z tests. At initial visit, all tests were significantly (p retinal vessel diameter in OD reduced gradually to reach statistical significance at the 5th visit and 6th visit (21 and 22 years after the first examination, respectively). In OD, the amplitude of the scotopic and photopic ERGs reduced gradually and was significantly smaller than normal at the 2nd visit (after 11 years) and 3rd visit (after 18 years), respectively. When the photopic ERG was analyzed using the discrete wavelet transform (DWT), we were able to detect a significant change at the 2nd visit (after 11 years) instead of the 3rd visit (18 years). Our study allowed us to witness the earliest manifestation of an RP disease process. The ERG was the first test to detect significant RP changes. A significantly earlier detection of ERG anomalies was obtained when the DWT was used, demonstrating its advantage for early detection of ERG changes.

RP105 Protects Against Apoptosis in Ischemia/Reperfusion-Induced Myocardial Damage in Rats by Suppressing TLR4-Mediated Signaling Pathways

Directory of Open Access Journals (Sweden)

Jun Yang

2015-07-01

Full Text Available Background: Myocardial apoptosis is heavily implicated in the myocardial damage caused by ischemia-reperfusion (I/R. Toll-like receptor 4 (TLR4 is a potent inducer of these apoptotic cascades. In contrast, the radioprotective 105 kDa protein (RP105 is a specific negative regulator of TLR4 signaling pathways. However, the precise mechanisms by which RP105 inhibits myocardium apoptosis via TLR4-associated pathways during I/R is not fully understood. Methods: We utilized a rat model of myocardial ischemic reperfusion injury (MIRI. Animals were pre-treated with Ad-EGFP adenovirus, Ad-EGFP-RP105 adenovirus, saline, or nothing (sham. After three days, rats underwent a 30min left anterior descending coronary artery occlusion and a 4h reperfusion. Mycardial tissue was assessed by immunohistochemistry, TUNEL-staining, Western blot, quantitative RT-PCR, and a morphometric assay. Results: RP105 overexpression resulted in a reduction in infarct size, fewer TUNEL-positive cardiomyocytes, and a reduction in mitochondrial-associated apoptosis cascade activity. Further, RP105 overexpression repressed I/R-induced myocardial injury by attenuating myocardial apoptosis. This was mediated by inhibiting TLR4 activation and the phosphorylation of P38MAPK and the downstream transcription factor AP-1. Conclusion: RP105 overexpression leads to the de-activation of TLR4, P38MAPK, and AP-1 signaling pathways, and subsequently represses apoptotic cascades and ensuing damage of myocardial ischemic reperfusion. These findings may become the basis of a novel therapeutic approach for reducing of cardiac damage caused by MIRI.

Leucocyte labeling: Preliminary clinical evaluation of RP517, the 99mTc-Leukotriene B4 receptor antagonist for infection/inflammation

International Nuclear Information System (INIS)

Soroa, V.E.; Velasquez, M.H.; Nicolini, J.; Ughetti, R.; Camin, L.

2002-01-01

99mTc- Leukotriene B4 (LTB4) is a receptor antagonist RP517 that labels predominantly neutrophils, in whole blood. This new radiopharmaceutical has been developed for imaging infection and inflammation. Aim: We conducted this clinical trial to obtain the best protocol imaging and the efficacy of this novel agent in the detection of suspected infected/inflamed patients. Materials and Methods: RP517 was labelled with 15-20 mCi (555-740 MBq) 99mTc for each patient, with a labeling efficiency of 86-95%. Nine patients studied (F 3, M 6) with ages 45-83, suspected of bone, joint and soft tissue infection (8) and one with fever of unknown origin (FUO). Laboratory, anatomical images and one other scintiscan (MDP, Gallium or 99mTc-ciprofloxacin) were used for evaluation. Acquisition protocol: flow, 30 min, 1, 2, 3, 4 - 24h planar or SPECT images if required. Results were compared to histology, cultures, the clinical derivation and follow up. Scans were classified as positive if abnormal uptake persisted through the 24h images or negative when absent pathological uptake or when positivity faded in later images. Results: No adverse reaction encountered. Image protocol modified to a flow, 30 min, 4 and 24h acquisitions. Early bladder excretion and intestinal activity requires previous cleansing enemas and food restrain. Late bone marrow activity is less visible than with in vitro leucocyte labeling. Coincident images were obtained in 3 True Positive, in 5 True Negative except in a hip prosthesis where Gallium and 99mTc-ciprofloxacin showed positivity and RP517 was False negative. Conclusions: A kit should be produced in order to make it available for Nuclear Medicine Departments. Known advantages of RP517 is the lack of in vitro cell labeling manipulation. We do not recommend investigation of FUO or abdominal pathologies. A larger series must be studied in order to be able to obtain statistics on joint bone and soft tissue infection/inflammation where this compound seems to be

Development of a New RP-UPLC Method for the Determination of ...

African Journals Online (AJOL)

Development of a New RP-UPLC Method for the Determination of Rabeprazole Sodium in Pharmaceutical Formulation and Application in Dissolution Studies. ... particle size 1.7 μm) column using an isocratic method with mobile phase composed of acetonitrile and phosphate buffer (pH 7.4) in the ratio of 35:65 (v/v).

Putative digenic inheritance of heterozygous RP1L1 and C2orf71 null mutations in syndromic retinal dystrophy

DEFF Research Database (Denmark)

Liu, Yangfan P.; Bosch, Daniëlle G.M.; Siemiatkowska, Anna M.

2017-01-01

Background: Retinitis pigmentosa (RP) is the most common cause of inherited retinal degeneration and can occur in non-syndromic and syndromic forms. Syndromic RP is accompanied by other symptoms such as intellectual disability, hearing loss, or congenital abnormalities. Both forms are known to ex...

R&P: the multiple meaning of a research project in general practice.

Science.gov (United States)

Visentin, Giorgio

2005-08-01

Rischio e Prevenzione (Risk and Prevention) is a research project that is becoming the paradigm of the Italian research on General Practice. It started from a survey showing that treatment and control of cardiovascular risk is still far from optimal even in very high-risk patients. A group of general practitioners, coordinated by Istituto Mario Negri, wrote the protocol of the study with various proposals: Creating a research network. Building research infrastructure with good research capacity. Building a 'therapeutic alliance' with the patient while presenting the research, not only obtaining their signature for a 'bureaucratic' informed consent. Having the 'Collaborative Group' as the 'sponsor' of a research even if the funds are coming from Pharmaceutical Industry. It is a randomised controlled trial (RCT) carried out in primary care with the normal patient of our daily work, so transferability is very possible. The way to enroll the patients and the request to specify the reason for not joining the project of the outcome study are a kind of participatory research. The outcome study can become a model for implementing new strategy on cardiovascular risk. A specific questionnaire will enquire the different point of view of the patient and of the general practitioner/researcher. The result of this project will help us understand the phenomenon of the poor compliance of the high-risk patients. First results during enrollment allow some optimism.

Quantitative analysis of rutin, quercetin, naringenin, and gallic acid by validated RP- and NP-HPTLC methods for quality control of anti-HBV active extract of Guiera senegalensis.

Science.gov (United States)

Alam, Perwez; Parvez, Mohammad K; Arbab, Ahmed H; Al-Dosari, Mohammed S

2017-12-01

Guiera senegalensis J.F. Gmel (Combretaceae) is a folk medicinal plant used in various metabolic and infectious diseases. In addition to its antiviral activities against herpes and fowlpox, the anti-HBV efficacy is very recently reported. To develop and validate simple, sensitive RP-/NP-HPTLC methods for quantitative determination of biomarkers rutin, quercetin, naringenin, and gallic acid in the anti-HBV active G. senegalensis leaves ethanol-extract. RP-HPTLC (rutin & quercetin; phase- acetonitrile:water, 4:6) and NP-HPTLC (naringenin & gallic acid; phase- toluene:ethyl acetate:formic acid, 6:4:0.8) were performed on glass-backed silica gel plates 60F 254 -RP18 and 60F 254 , respectively. The methods were validated according to the ICH guidelines. Well-separated and compact spots (R f ) of rutin (0.52 ± 0.006), quercetin (0.23 ± 0.005), naringenin (0.56 ± 0.009) and gallic acid (0.28 ± 0.006) were detected. The regression equations (Y) were 12.434x + 443.49, 10.08x + 216.85, 11.253x + 973.52 and 11.082x + 446.41 whereas the coefficient correlations (r 2 ) were 0.997 ± 0.0004, 0.9982 ± 0.0001, 0.9974 ± 0.0004 and 0.9981 ± 0.0001, respectively. The linearity ranges (ng/spot) were 200-1400 (RP-HPTLC) and 100-1200 (NP-HPTLC). The LOD/LOQ (ng/band) were 33.03/100.1 (rutin), 9.67/29.31 (quercetin), 35.574/107.8 (naringenin), and 12.32/37.35 (gallic acid). Gallic acid (7.01 μg/mg) was the most abundant biomarker compared to rutin (2.42 μg/mg), quercetin (1.53 μg/mg) and naringenin (0.14 μg/mg) in the extract. The validated NP-/RP-HPTLC methods were simple, accurate, and sensitive for separating and quantifying antiviral biomarkers in G. senegalensis, and endorsed its anti-HBV activity. The developed methods could be further employed in the standardization and quality-control of herbal formulations.

RP TLC-based lipophilicity assessment of some natural and synthetic coumarins

Energy Technology Data Exchange (ETDEWEB)

Rabtti, El Hadi M.A.; Natic, Maja M.; Milojkovic-Opsenica, Dusanka M.; Trifkovic, Jelena D.; Vuckovic, Ivan M.; Tesic, Zivoslav Lj., E-mail: ztesic@chem.bg.ac.rs [Faculty of Chemistry, University of Belgrade, Belgrade (Serbia); Vajs, Vlatka E. [Institute of Chemistry, Technology and Metallurgy, University of Belgrade, Belgrade (Serbia)

2012-03-15

The lipophilic character of twelve coumarins was investigated by reversed-phase thin-layer chromatography (RP TLC) on RP-18 silica. The three different binary solvent systems composed of water and organic modifier (methanol, tetrahydrofuran or acetonitrile) were used in order to determine retention parameter (R{sub M}{sup 0}) and octanol-water partition coefficient (log P{sub OW})as a measure of the lipophilicity of the tested compounds. Lipophilicity parameter (log P{sub OW}) was experimentally determined using eight standard solutes with known log P{sub OW} values which were analyzed under the same chromatographic conditions as the target substances. Lipophilicity parameters together with 2D molecular descriptors were subjected to the multivariate statistical analysis (principal component analysis (PCA) and partial least square (PLS) regression) in order to reveal the most influential factors governing the retention, i.e., lipophilicity of the investigated compounds. The quantitative structure-retention relationship models reveal the importance of descriptors which describe the size and the shape of the molecule as well as their polar properties. (author)

Proposing a project for building an e-commerce website for Ryijypalvelu-RP Oy

OpenAIRE

Sotkov, Ilya

2014-01-01

The thesis describes the development of a project proposal report for building an e-commerce website for a small textile firm based in Helsinki, Finland. The aim of the study is to determine how small businesses that are currently not highly involved in e-commerce should approach developing an e-commerce website in order to maximize the positive impact of selling on the Internet. The study combined the use of action research and qualitative research methodologies by developing a project p...

Development and validation of a stability-indicating RP-HPLC method for determination of atomoxetine hydrochloride in tablets.

Science.gov (United States)

Patel, Sejal K; Patel, Natvarlal J

2010-01-01

This paper describes the development of a stability-indicating RP-HPLC method for the determination of atomoxetine hydrochloride (ATX) in the presence of its degradation products generated from forced decomposition studies. The drug substance was subjected to stress conditions of acid, base, oxidation, wet heat, dry heat, and photodegradation. In stability tests, the drug was susceptible to acid, base, oxidation, and dry and wet heat degradation. It was found to be stable under the photolytic conditions tested. The drug was successfully separated from the degradation products formed under stress conditions on a Phenomenex C18 column (250 x 4.6 mm id, 5 microm particle size) by using acetonitrile-methanol-0.032 M ammonium acetate (55 + 05 + 40, v/v/v) as the mobile phase at 1.0 mL/min and 40 degrees C. Photodiode array detection at 275 nm was used for quantitation after RP-HPLC over the concentration range of 0.5-5 microg/mL with a mean recovery of 100.8 +/- 0.4% for ATX. Statistical analysis demonstrated that the method is repeatable, specific, and accurate for the estimation of ATX. Because the method effectively separates the drug from its degradation products, it can be used as a stability-indicating method.

Development of a definition for Rapid Progression (RP) of renal function in HIV-positive persons : the D:A:D study

NARCIS (Netherlands)

Kamara, David A; Ryom, Lene; Ross, Michael; Kirk, Ole; Reiss, Peter; Morlat, Philippe; Moranne, Olivier; Fux, Christoph A; Mocroft, Amanda; Sabin, Caroline; Lundgren, Jens D; Smith, Colette J; Schölvinck, Elisabeth H.

2014-01-01

BACKGROUND: No consensus exists on how to define abnormally rapid deterioration in renal function (Rapid Progression, RP). We developed an operational definition of RP in HIV-positive persons with baseline estimated glomerular filtration rate (eGFR) >90 ml/min/1.73 m2 (using Cockcroft Gault) in the

Development of a definition for Rapid Progression (RP) of renal function in HIV-positive persons: the D:A:D study

NARCIS (Netherlands)

Kamara, David A.; Ryom, Lene; Ross, Michael; Kirk, Ole; Reiss, Peter; Morlat, Philippe; Moranne, Olivier; Fux, Christoph A.; Mocroft, Amanda; Sabin, Caroline; Lundgren, Jens D.; Smith, Colette J.; Powderly, B.; Shortman, N.; Moecklinghoff, C.; Reilly, G.; Franquet, X.; Ryom, L.; Sabin, C. A.; Kamara, D.; Smith, C.; Phillips, A.; Mocroft, A.; Tverland, J.; Mansfeld, M.; Nielsen, J.; Raben, D.; Lundgren, J. D.; Brandt, R. Salbøl; Rickenbach, M.; Fanti, I.; Krum, E.; Hillebregt, M.; Geffard, S.; Sundström, A.; Prins, J. M.; Kuijpers, T. W.; Scherpbier, H. J.; van der Meer, J. T. M.; Wit, F. W. M. N.; Godfried, M. H.; Nellen, F. J. B.; Lange, J. M. A.; Geerlings, S. E.; van Vugt, M.; Pajkrt, D.; Grijsen, M. L.; Wiersinga, W. J.; Goorhuis, A.; Hovius, J. W. R.

2014-01-01

No consensus exists on how to define abnormally rapid deterioration in renal function (Rapid Progression, RP). We developed an operational definition of RP in HIV-positive persons with baseline estimated glomerular filtration rate (eGFR) >90 ml/min/1.73 m2 (using Cockcroft Gault) in the Data

Development of a definition for Rapid Progression (RP) of renal function in HIV-positive persons: the D:A:D study

NARCIS (Netherlands)

Kamara, D.A.; Ryom, L.; Ross, M.; Kirk, O.; Reiss, P.; Morlat, P.; Moranne, O.; Fux, C.A.; Mocroft, A.; Sabin, C.; Lundgren, J.D.; Smith, C.J.; Koopmans †, P.P.; Keuter, M.; Ven, A.J.A.M. van der; Hofstede, H.J.M. ter; Dofferhoff, A.S.M.; Warris, A.; Crevel, R. van; et al.,

2014-01-01

BACKGROUND: No consensus exists on how to define abnormally rapid deterioration in renal function (Rapid Progression, RP). We developed an operational definition of RP in HIV-positive persons with baseline estimated glomerular filtration rate (eGFR) >90 ml/min/1.73 m2 (using Cockcroft Gault) in the

6 Project-Management Tips

Science.gov (United States)

Demski, Jennifer

2012-01-01

When it comes to project management, the IT department is typically its own worst enemy. When project requests are pushed through the budgeting process by different departments, it's up to IT to make them all work. The staff is required to be "heroic" to get the project load done. People get to work over weekends and postpone their vacations. The…

The role of GluN2A and GluN2B NMDA receptor subunits in AgRP and POMC neurons on body weight and glucose homeostasis.

Science.gov (United States)

Üner, Aykut; Gonçalves, Gabriel H M; Li, Wenjing; Porceban, Matheus; Caron, Nicole; Schönke, Milena; Delpire, Eric; Sakimura, Kenji; Bjørbæk, Christian

2015-10-01

Hypothalamic agouti-related peptide (AgRP) and pro-opiomelanocortin (POMC) expressing neurons play critical roles in control of energy balance. Glutamatergic input via n-methyl-d-aspartate receptors (NMDARs) is pivotal for regulation of neuronal activity and is required in AgRP neurons for normal body weight homeostasis. NMDARs typically consist of the obligatory GluN1 subunit and different GluN2 subunits, the latter exerting crucial differential effects on channel activity and neuronal function. Currently, the role of specific GluN2 subunits in AgRP and POMC neurons on whole body energy and glucose balance is unknown. We used the cre-lox system to genetically delete GluN2A or GluN2B only from AgRP or POMC neurons in mice. Mice were then subjected to metabolic analyses and assessment of AgRP and POMC neuronal function through morphological studies. We show that loss of GluN2B from AgRP neurons reduces body weight, fat mass, and food intake, whereas GluN2B in POMC neurons is not required for normal energy balance control. GluN2A subunits in either AgRP or POMC neurons are not required for regulation of body weight. Deletion of GluN2B reduces the number of AgRP neurons and decreases their dendritic length. In addition, loss of GluN2B in AgRP neurons of the morbidly obese and severely diabetic leptin-deficient Lep (ob/ob) mice does not affect body weight and food intake but, remarkably, leads to full correction of hyperglycemia. Lep (ob/ob) mice lacking GluN2B in AgRP neurons are also more sensitive to leptin's anti-obesity actions. GluN2B-containing NMDA receptors in AgRP neurons play a critical role in central control of body weight homeostasis and blood glucose balance via mechanisms that likely involve regulation of AgRP neuronal survival and structure, and modulation of hypothalamic leptin action.

Altered gut microbiota promotes colitis-associated cancer in IL-1 receptor-associated kinase M-deficient mice

Czech Academy of Sciences Publication Activity Database

Klimešová, Klára; Kverka, Miloslav; Zákostelská, Zuzana; Hudcovic, Tomáš; Hrnčíř, Tomáš; Štěpánková, Renata; Rossmann, Pavel; Rídl, Jakub; Kostovčík, Martin; Mrázek, Jakub; Kopečný, Jan; Kobayashi, K.; Tlaskalová-Hogenová, Helena

2013-01-01

Roč. 19, č. 6 (2013), s. 1266-1277 ISSN 1078-0998 R&D Projects: GA ČR(CZ) GAP304/11/1252; GA ČR GA303/08/0367; GA ČR GAP303/12/0535 Institutional support: RVO:61388971 ; RVO:68378050 ; RVO:67985904 Keywords : cancer in IBD * mucosal immunity * colorectal cancer Subject RIV: EC - Immunology; CE - Biochemistry (UMG-J); EE - Microbiology, Virology (UZFG-Y) Impact factor: 5.475, year: 2013

78 FR 65690 - Trees and Plantings Associated With Eligible Facilities, RP9524.5

Science.gov (United States)

2013-11-01

...] Trees and Plantings Associated With Eligible Facilities, RP9524.5 AGENCY: Federal Emergency Management... policy Trees and Plantings Associated with Eligible Facilities. The Federal Emergency Management Agency... trees, shrubs, and other plantings, including limited eligibility for replacement of grass and sod...

Molecular characterization and bio-functional property determination using SDS-PAGE and RP-HPLC of protein fractions from two Nigella species.

Science.gov (United States)

Alu'datt, Muhammad H; Rababah, Taha; Alhamad, Mohammad N; Alodat, Moh'd; Al-Mahasneh, Majdi A; Gammoh, Sana; Ereifej, Khalil; Almajwal, Ali; Kubow, Stan

2017-09-01

This study aimed to investigate the molecular and bio-functional properties of protein fractions from Nigella damascena and Nigella arvensis, including the albumin, globulin, glutein-1, glutein-2 and prolamin fractions. Protein subunits were not observed in globulin and prolamin fractions. No peaks appeared in RP-HPLC chromatograms of globulin for either species. Two predominant peaks were observed in the RP-HPLC profiles of all protein fractions. Proteins separated by RP-HPLC have potential inhibitory and antioxidant activities in all fractions. Optimum ACE-inhibitory and antioxidant activities of proteins separated by RP-HPLC were observed in glutein-2 and albumin, respectively, for both species. For pepsin and combined pepsin-trypsin hydrolyses, the highest degree of hydrolysis (DH) was obtained in glutein-2 fraction of Nigella arvensis. Highest ACE-inhibitory activity of hydrolyzed protein fractions was found at 4h via pepsin hydrolysis in globulin fraction of Nigella damascena. Highest antioxidant activities of hydrolyzed protein fractions were found in glutelin-2 for both species. Copyright © 2017 Elsevier Ltd. All rights reserved.

Characterization and adjustment of the neutron radiography facility of the RP-10 nuclear reactor

International Nuclear Information System (INIS)

Ravello R, Y.R.

2001-01-01

The main aim of this work was to characterize and adjust the neutron radiography facility of the RP-10 nuclear reactor, and therefore be able to offer with this technique services to the industry and research centers in general. This technique will be complemented with others such as x-rays and gamma radiography. First, the shielding capacity of the facility was analyzed, proving that it complies with the radiological safety requirements established by the radiological safety code. Then gamma filtration tests were conducted in order to implement the direct method for image formation, optical density curves were built according to the thickness of the gamma filter, the type of film and the type of irradiation. Also, the indirect method for image formation was implemented for two types of converters: indium and dysprosium. Growth curves for optical density were also made according to contact time between converter-film, for different types of films. The resolution of the facility was also analyzed using two methods: Klasens (1946) and Harms (1986). Harms method came closer to the resolution of the human eye when compared to the Klasens method. Finally, the application fields of neutron radiography are presented, including those conducted at the neutron radiography facility of the RP-10 nuclear reactor. With this work, the RP-10 neutron radiography facility is ready to offer inspection and research services

Very low levels of 6-keto-prostaglandin F 1/sub α/ in human plasma

International Nuclear Information System (INIS)

Siess, W.; Dray, F.

1982-01-01

Two stable derivatives of PGI 2 , its nonenzymatic hydrolysis product (6-keto-PGF 1 /sub α/) and an enzymatic metabolite (6, 15-diketo-PGF 1 /sub α/) were determined in human plasma and urine. These compounds were measured by RIA after separation on rp-HPLC. Previous purification of the samples on rp=HPLC markedly enhanced the specificity of the RIA determinations of those compounds in plasma and urine. The PGI 2 derivative 6-keto-PGF 1 /sub α/ was detected in both plasma (4.7 +/- 3.2 pg/ml, mean +/- S.D., n = 34) and urine (166 +/- 61 pg/ml, n = 9). No gender differences of the plasma or urinary levels of 6-keto-PGF 1 /sub α/ were found. The PGI 2 metabolite 6, 15-diketo-PGF 1 /sub α/ was not measurable in plasma or urine ( 2 in man. When [ 3 H]PGI 2 was added to citrated blood immediately after venipuncture, it was recovered entirely as [ 3 H]6-keto-PGF 1 /sub α/ after rp-HPLC. Therefore any circulating PGI 2 would be measured as 6-keto-PGF 1 /sub α/ by our method. The results obtained suggest that PGI 2 could be present in human venous blood under physiological conditions, but only in very low concentrations

A Validated RP-HPLC Method for the Determination of Atazanavir in Pharmaceutical Dosage Form

Directory of Open Access Journals (Sweden)

K. Srinivasu

2011-01-01

Full Text Available A validated RP HPLC method for the estimation of atazanavir in capsule dosage form on YMC ODS 150 × 4.6 mm, 5 μ column using mobile phase composition of ammonium dihydrogen phosphate buffer (pH 2.5 with acetonitrile (55:45 v/v. Flow rate was maintained at 1.5 mL/min with 288 nm UV detection. The retention time obtained for atazanavir was at 4.7 min. The detector response was linear in the concentration range of 30 - 600 μg/mL. This method has been validated and shown to be specific, sensitive, precise, linear, accurate, rugged, robust and fast. Hence, this method can be applied for routine quality control of atazanavir in capsule dosage forms as well as in bulk drug.

Lipopolysaccharide (LPS) and tumor necrosis factor alpha (TNFα) blunt the response of Neuropeptide Y/Agouti-related peptide (NPY/AgRP) glucose inhibited (GI) neurons to decreased glucose.

Science.gov (United States)

Hao, Lihong; Sheng, Zhenyu; Potian, Joseph; Deak, Adam; Rohowsky-Kochan, Christine; Routh, Vanessa H

2016-10-01

A population of Neuropeptide Y (NPY) neurons which co-express Agouti-related peptide (AgRP) in the arcuate nucleus of the hypothalamus (ARC) are inhibited at physiological levels of brain glucose and activated when glucose levels decline (e.g. glucose-inhibited or GI neurons). Fasting enhances the activation of NPY/AgRP-GI neurons by low glucose. In the present study we tested the hypothesis that lipopolysaccharide (LPS) inhibits the enhanced activation of NPY/AgRP-GI neurons by low glucose following a fast. Mice which express green fluorescent protein (GFP) on their NPY promoter were used to identify NPY/AgRP neurons. Fasting for 24h and LPS injection decreased blood glucose levels. As we have found previously, fasting increased c-fos expression in NPY/AgRP neurons and increased the activation of NPY/AgRP-GI neurons by decreased glucose. As we predicted, LPS blunted these effects of fasting at the 24h time point. Moreover, the inflammatory cytokine tumor necrosis factor alpha (TNFα) blocked the activation of NPY/AgRP-GI neurons by decreased glucose. These data suggest that LPS and TNFα may alter glucose and energy homeostasis, in part, due to changes in the glucose sensitivity of NPY/AgRP neurons. Interestingly, our findings also suggest that NPY/AgRP-GI neurons use a distinct mechanism to sense changes in extracellular glucose as compared to our previous studies of GI neurons in the adjacent ventromedial hypothalamic nucleus. Copyright © 2016 Elsevier B.V. All rights reserved.

Project management approach for the Waste Area Grouping 6 Closure/Remediation Project at Oak Ridge National Laboratory, Oak Ridge, Tennessee

International Nuclear Information System (INIS)

1992-07-01

This document has been developed as a preliminary definition of the Waste Area Grouping (WAG) 6 Closure Project Management Approach. The purpose of this document is to identify the roles and responsibilities of the various project team members and to identify the project scope, schedule and budget. This document is intended to be a living document. As information develops, this document will be revised to create a WAG 6 Project Management Plan (PMP). The PMP will provide additional focus to the information contained in this document. The information required will be available as the selected alternative for remediation of WAG 6 is approved and Remedial Action Plans are conceptualized. This document has been reviewed against, and is intended to be consistent with, the Environmental Restoration Program Management Plan

Ribosomal protein mutations induce autophagy through S6 kinase inhibition of the insulin pathway.

Directory of Open Access Journals (Sweden)

Harry F Heijnen

Full Text Available Mutations affecting the ribosome lead to several diseases known as ribosomopathies, with phenotypes that include growth defects, cytopenia, and bone marrow failure. Diamond-Blackfan anemia (DBA, for example, is a pure red cell aplasia linked to the mutation of ribosomal protein (RP genes. Here we show the knock-down of the DBA-linked RPS19 gene induces the cellular self-digestion process of autophagy, a pathway critical for proper hematopoiesis. We also observe an increase of autophagy in cells derived from DBA patients, in CD34+ erythrocyte progenitor cells with RPS19 knock down, in the red blood cells of zebrafish embryos with RP-deficiency, and in cells from patients with Shwachman-Diamond syndrome (SDS. The loss of RPs in all these models results in a marked increase in S6 kinase phosphorylation that we find is triggered by an increase in reactive oxygen species (ROS. We show that this increase in S6 kinase phosphorylation inhibits the insulin pathway and AKT phosphorylation activity through a mechanism reminiscent of insulin resistance. While stimulating RP-deficient cells with insulin reduces autophagy, antioxidant treatment reduces S6 kinase phosphorylation, autophagy, and stabilization of the p53 tumor suppressor. Our data suggest that RP loss promotes the aberrant activation of both S6 kinase and p53 by increasing intracellular ROS levels. The deregulation of these signaling pathways is likely playing a major role in the pathophysiology of ribosomopathies.

RESTING PLASMA AgRP LEVELS RESPONSE TO EXERCISE-CONJUGATED DIET AND ONLY DIET IN OVERWEIGHT AND OBESE SEDENTARY FEMALES

Directory of Open Access Journals (Sweden)

Mahmoud Delphan

2012-01-01

Full Text Available Agouti-related peptide (AgRP is an orexigenic neuropeptide produced mainly in the arcuate nucleus (ARC of the hypothalamus, which leads to positive energy balance. The purpose of the present study was to investigate the resting plasma AgRP levels’ response to exercise-conjugated diet and only diet in overweight and obese sedentary female college students. Thirty subjects were randomly divided into two experimental groups and one control group. Experimental group 1 experienced 12 days of researchers’ proposed diet (D, experimental group 2 experienced 12 days of researchers’ proposed diet (every day with 12-day running training (the intensity was 60 to 70�0heart rate maximum for 50 minutes every other day (AED and the control group remained sedentary with a normal diet in this period. Blood samples were collected 24 hours before and after the enforced protocol. Body fat percentage (BF�20of subjects was measured using the 3-point method. Plasma AgRP levels were measured using the enzyme-linked immunosorbent assay (ELISA method. The data were analysed by Leven, Kolmogorov-Smirnov, one-way analysis of variance (ANOVA and Tukey post-hoc test. At the end of the protocols, the resting plasma AgRP in the D group increased significantly (p0.05. Body weight and BMI were significantly reduced for both experimental groups (P<0.001. Body fat percentage in the AED group was decreased (p<0.001 and no change was observed in the D group. The present results indicate that the AED is better than the D protocol because it leads to stability of AgRP, which may prevent lipogenesis and ultimately reduce body fat percentage.

Identification of a novel p.R1443W mutation in RP1 gene associated with retinitis pigmentosa sine pigmento

Directory of Open Access Journals (Sweden)

Li Ma

2013-08-01

Full Text Available AIM: To screen mutations in the retinitis pigmentosa 1 (RP1 gene and the rhodopsin (RHO gene in Chinese patients with retinitis pigmentosa sine pigmento (RPSP and describe the genotype-phenotype relationship of the mutations.METHODS:Twenty affected, unrelated Chinese individuals with RPSP (4 autosomal dominant RPSP, 12 autosomal recessive RPSP and 4 unknown inheritance pattern were recruited between 2009 and 2012. The clinical features were determined by complete ophthalmologic examinations. Polymerase chain reaction (PCR and direct DNA sequencing were used to screen the entire coding region and splice junctions of the RP1 gene and the RHO gene. The cosegregation analysis and population frequency studies were performed for patients with identified mutations.RESULTS: Five variants in the RP1 gene and one in the RHO gene were detected in 20 probands. Four missense changes (rs444772, rs446227, rs414352, rs441800 and one non-coding variant (rs56340615 were common SNPs and none of them showed a significant relationship with RPSP. A missense mutation p.R1443W was identified in the RP1 gene in three affected individuals from a family with autosomal dominant RPSP and was found to cosegregate with the phenotype in this family, suggestive of pathogenic. In addition, population frequency analysis showed the p.R1443W mutation was absent in 300 healthy controls.CONCLUSION: The identification of p.R1443W mutation cosegregating in a family with autosomal dominant RPSP highlights an atypical phenotype of the RP1 gene mutation, while RHO gene is not associated with the pathogenesis of RPSP in this study. To our knowledge, this is the fist mutation identified to associate with RPSP.

Identification of a novel p.R1443W mutation in RP1 gene associated with retinitis pigmentosa sine pigmento.

Science.gov (United States)

Ma, Li; Sheng, Xun-Lun; Li, Hui-Ping; Zhang, Fang-Xia; Liu, Ya-Ni; Rong, Wei-Ning; Zhang, Jian-Ling

2013-01-01

To screen mutations in the retinitis pigmentosa 1 (RP1) gene and the rhodopsin (RHO) gene in Chinese patients with retinitis pigmentosa sine pigmento (RPSP) and describe the genotype-phenotype relationship of the mutations. Twenty affected, unrelated Chinese individuals with RPSP (4 autosomal dominant RPSP, 12 autosomal recessive RPSP and 4 unknown inheritance pattern) were recruited between 2009 and 2012. The clinical features were determined by complete ophthalmologic examinations. Polymerase chain reaction (PCR) and direct DNA sequencing were used to screen the entire coding region and splice junctions of the RP1 gene and the RHO gene. The cosegregation analysis and population frequency studies were performed for patients with identified mutations. Five variants in the RP1 gene and one in the RHO gene were detected in 20 probands. Four missense changes (rs444772, rs446227, rs414352, rs441800) and one non-coding variant (rs56340615) were common SNPs and none of them showed a significant relationship with RPSP. A missense mutation p.R1443W was identified in the RP1 gene in three affected individuals from a family with autosomal dominant RPSP and was found to cosegregate with the phenotype in this family, suggestive of pathogenic. In addition, population frequency analysis showed the p.R1443W mutation was absent in 300 healthy controls. The identification of p.R1443W mutation cosegregating in a family with autosomal dominant RPSP highlights an atypical phenotype of the RP1 gene mutation, while RHO gene is not associated with the pathogenesis of RPSP in this study. To our knowledge, this is the fist mutation identified to associate with RPSP.

Education and training in radiological protection: the activities of the isRP

International Nuclear Information System (INIS)

Coeck, M.

2005-01-01

The International School for Radiological Protection, a task force within SCK-CEN, co-ordinates and organises training programs on all aspects of radiological protection. IsRP courses are directed as well to the private sector as to the political and academic world and the general public. International meetings, publications and recommendations with regard to safety culture increasingly stress the importance of education and training in the field of radiological protection. In addition, the need to standardise and harmonise the recognition of skills and practices on a national and European level is emerging. In this sense, the objectives of isRP are threefold : (1) to continue the organisation of open courses and training-on-demand of which the programmes are made up according to the background level and training requirements of the participants, and this for the Belgian medical and industrial sector; (2) to come to a closer cooperation with national universities and high schools and relevant international institutions; (3) to contribute to a better harmonisation of training practice and of skills recognition on a national and European level

NPY/AgRP neurons are not essential for feeding responses to glucoprivation.

Science.gov (United States)

Luquet, Serge; Phillips, Colin T; Palmiter, Richard D

2007-02-01

Animals respond to hypoglycemia by eating and by stimulating gluconeogenesis. These responses to glucose deprivation are initiated by glucose-sensing neurons in the brain, but the neural circuits that control feeding behavior are not well established. Neurons in the arcuate region of the hypothalamus that express neuropeptide Y (NPY) and agouti-related protein (AgRP) have been implicated in mediating the feeding response to glucoprivation. We devised a method to selectively ablate these neurons in neonatal mice and then tested adult mice for their feeding responses to fasting, mild hypoglycemia, 2-deoxy-d-glucose and a ghrelin receptor agonist. Whereas the feeding response to the ghrelin receptor agonist was completely abrogated, the feeding response to glucoprivation was normal. The feeding response after a fast was attenuated when standard chow was available but normal with more palatable solid or liquid diet. We conclude that NPY/AgRP neurons are not necessary for generating or mediating the orexigenic response to glucose deficiency, but they are essential for the feeding response to ghrelin and refeeding on standard chow after a fast.

Treatment of Brodie's Syndrome using parasymphyseal distraction through virtual surgical planning and RP assisted customized surgical osteotomy guide-A mock surgery report

Science.gov (United States)

Dahake, Sandeep; Kuthe, Abhaykumar; Mawale, Mahesh

2017-10-01

This paper aims to describe virtual surgical planning (VSP), computer aided design (CAD) and rapid prototyping (RP) systems for the preoperative planning of accurate treatment of the Brodie's Syndrome. 3D models of the patient's maxilla and mandible were separately generated based on computed tomography (CT) image data and fabricated using RP. During the customized surgical osteotmy guide (CSOG) design process, the correct position was identified and the geometry of the CSOG was generated based on affected mandible of the patient and fabricated by a RP technique. Surgical approach such as preoperative planning and simulation of surgical procedures was performed using advanced software. The VSP and RP assisted CSOG was used to avoid the damage of the adjacent teeth and neighboring healthy tissues. Finally the mock surgery was performed on the biomodel (i.e. diseased RP model) of mandible with reference to the normal maxilla using osteotomy bur with the help of CSOG. Using this CSOG the exact osteotomy of the mandible and the accurate placement of the distractor were obtained. It ultimately improved the accuracy of the surgery in context of the osteotomy and distraction. The time required in cutting the mandible and placement of the distractor was found comparatively less than the regular free hand surgery.

Identification of a nuclear localization signal in the retinitis pigmentosa-mutated RP26 protein, ceramide kinase-like protein

International Nuclear Information System (INIS)

Inagaki, Yuichi; Mitsutake, Susumu; Igarashi, Yasuyuki

2006-01-01

Retinitis pigmentosa (RP) is a genetically heterogeneous disease characterized by degeneration of the retina. A mutation in a new ceramide kinase (CERK) homologous gene, named CERK-like protein (CERKL), was found to cause autosomal recessive retinitis pigmentosa (RP26). Here, we show a point mutation of one of two putative nuclear localization signal (NLS) sequences inhibited the nuclear localization of the protein. Furthermore, the tetra-GFP-tagged NLS, which cannot passively enter the nucleus, was observed not only in the nucleus but also in the nucleolus. Our results provide First evidence of the active nuclear import of CERKL and suggest that the identified NLS might be responsible for nucleolar retention of the protein. As recent studies have shown other RP-related proteins are localized in the nucleus or the nucleolus, our identification of NLS in CERKL suggests that CERKL likely plays important roles for retinal functions in the nucleus and the nucleolus

The Structural Basis for Lipid and Endotoxin Binding in RP105-MD-1, and Consequences for Regulation of Host Lipopolysaccharide Sensitivity.

Science.gov (United States)

Ortiz-Suarez, Maite L; Bond, Peter J

2016-01-05

MD-1 is a member of the MD-2-related lipid-recognition (ML) family, and associates with RP105, a cell-surface protein that resembles Toll-like receptor 4 (TLR4). The RP105⋅MD-1 complex has been proposed to play a role in fine-tuning the innate immune response to endotoxin such as bacterial lipopolysaccharide (LPS) via TLR4⋅MD-2, but controversy surrounds its mechanism. We have used atomically detailed simulations to reveal the structural basis for ligand binding and consequent functional dynamics of MD-1 and the RP105 complex. We rationalize reports of endogenous phospholipid binding, by showing that they prevent collapse of the malleable MD-1 fold, before refining crystallographic models and uncovering likely binding modes for LPS analogs. Subsequent binding affinity calculations reveal that endotoxin specificity arises from the entropic cost of expanding the MD-1 cavity to accommodate bulky lipid tails, and support the role of MD-1 as a "sink" that sequesters endotoxin from TLR4 and stabilizes RP105/TLR4 interactions. Copyright © 2016 Elsevier Ltd. All rights reserved.

[Quantative analysis of eupatilin and jaceosidin in folium of Artemisia argyi from different areas in China by RP-HPLC based on ancient medicine books].

Science.gov (United States)

Huang, Xian-Zhang; Kang, Li-Ping; Gao, Li; Zhang, Yuan; Guo, Lan-Ping; Huang, Lu-Qi

2017-09-01

In order to evaluate the quality of Artemisia argyi from Qichun, Ningbo, Anguo and Nanyang, the contents of eupatilin and jaceosidin were determined by RP-HPLC. The determination was performed on Agilent Eclipse XDB-C₁₈ (4.6 mm×250 mm, 5 μm) with mobile phase consisted of acetonitrile-0.2% phosphoric acid(35∶65) at the flow rate 1.0 mL•min ⁻¹. The detection wavelength was 350 nm and the column temperature was 25 ℃. The results showed that the amount of eupatilin and jaceosidin had a clear linear relationship in the range of 0.003-0.126 g•L ⁻¹ (r=0.999 9) and 0.005-0.200 g•L ⁻¹ (r=0.999 9), and the average recovery rates for them were 99.14% (n=6, RSD 1.2%) and 99.40% (n=6, RSD=0.73%), respectively. The results showed that RP-HPLC can be used for the quantification of eupatilin and jaceosidin in the folium of A. argyi. With this method, we found there was no significant difference of jaceosidin content within all the samples collected, but the content of eupatilin was significantly higher in samples from Qichun, Ningbo, Xiangyang and Nanyang, located in the south of Huaihe River compared with these from other areas. Copyright© by the Chinese Pharmaceutical Association.

High-Temperature Nucleosynthesis Processes on the Proton-Rich Side of Stability: the Alpha-Rich Freezeout and the rp^2-Process

Science.gov (United States)

Meyer, Bradley S.

2001-10-01

Nucleosynthesis on the proton-rich side of stability has at least two intriguing aspects. First, the most abundant of the stable iron-group isotopes, such as ^48Ti, ^52Cr, and ^56,57Fe, are synthesized as proton-rich, radioactive parents in alpha-rich freezeouts from equilibrium. The production of these radioactive progenitors depends in large measure on reactions on the proton-rich side of stability. The second intriguing aspect is that explosive nucleosynthesis in a hydrogen-rich environment (namely, the rp-process) may be associated with exotic astrophysical settings, such as x-ray bursts, and may be responsible for production of some of the light p-process nuclei (for example, ^92,94Mo and ^96,98Ru). We have developed web-based tools to help nuclear physicists determine which nuclear reactions on the proton-rich side of stability govern the nucleosynthesis in these processes. For the alpha-rich freezeout, one may determine the effect of any one of 2,140 reactions on the yield of any isotope in the nuclear reaction network with the web calculator. As a relevant example, I will discuss the governing role of ^57Ni (n,p)^57Co in the synthesis of the important astronomical observable ^57Co. As for explosive, proton-rich burning, I will discuss the synthesis of p-process nuclei in the repetitive rp-process (the rp^2-process). movies/rp.html>Movies of the rp^2-process illustrate its important features and give some indications of the important nuclear reactions.

A comparison between customized clear and removable orthodontic appliances manufactured using RP and CNC techniques.

Science.gov (United States)

Martorelli, Massimo; Gerbino, Salvatore; Giudice, Michele; Ausiello, Pietro

2013-02-01

Aim of the research is to compare the orthodontic appliances fabricated by using rapid prototyping (RP) systems, in particular 3D printers, with those manufactured by using computer numerical control (CNC) milling machines. 3D printing is today a well-accepted technology to fabricate orthodontic aligners by using the thermoforming process, instead the potential of CNC systems in dentistry have not yet been sufficiently explored. One patient, with mal-positioned maxillary central and lateral incisors, was initially selected. In the computer aided virtual planning was defined that, for the treatment, the patient needed to wear a series of 7 removable orthodontic appliances (ROA) over a duration of 21 weeks, with one appliance for every 3 weeks. A non-contact reverse engineering (RE) structured-light 3D scanner was used to create the 3D STL model of the impression of the patient's mouth. Numerical FEM simulations were performed varying the position of applied forces (discrete and continuous forces) on the same model, simulating, in this way, 3 models with slice thickness of 0.2 mm, 0.1 mm (RP staircase effect) and without slicing (ideal case). To define the areas of application of forces, two configuration "i" and "i-1" of the treatment were overlapped. 6 patients to which for three steps (3rd, 4th and 5th step) were made to wear aligners fabricated starting from physical models by 3D printing (3DP-ROA) and afterwards, for the next steps (6th, 7th and 8th step), aligners fabricated starting from physical models by CNC milling machine (CNC-ROA), were selected. For the 6 patients wearing the CNC-ROA, it was observed a best fitting of the aligner to the teeth and a more rapid teeth movement than the 3DP-ROA (2 weeks compared to 3 weeks for every appliance). FEM simulations showed a more uniform stress distribution for CNC-ROA than 3DP-ROA. In this research, 6 different case studies and CAD-FEM simulations showed that, to fabricate an efficient clear and removable

The Scenario Model Intercomparison Project (ScenarioMIP) for CMIP6

Energy Technology Data Exchange (ETDEWEB)

O' Neill, Brian C.; Tebaldi, Claudia; van Vuuren, Detlef P.; Eyring, Veronika; Friedlingstein, Pierre; Hurtt, George; Knutti, Reto; Kriegler, Elmar; Lamarque, Jean-Francois; Lowe, Jason; Meehl, Gerald A.; Moss, Richard; Riahi, Keywan; Sanderson, Benjamin M.

2016-01-01

Projections of future climate change play a fundamental role in improving understanding of the climate system as well as characterizing societal risks and response options. The Scenario Model Intercomparison Project (ScenarioMIP) is the primary activity within Phase 6 of the Coupled Model Intercomparison Project (CMIP6) that will provide multi-model climate projections based on alternative scenarios of future emissions and land use changes produced with integrated assessment models. In this paper, we describe ScenarioMIP's objectives, experimental design, and its relation to other activities within CMIP6. The ScenarioMIP design is one component of a larger scenario process that aims to facilitate a wide range of integrated studies across the climate science, integrated assessment modeling, and impacts, adaptation, and vulnerability communities, and will form an important part of the evidence base in the forthcoming Intergovernmental Panel on Climate Change (IPCC) assessments. At the same time, it will provide the basis for investigating a number of targeted science and policy questions that are especially relevant to scenario-based analysis, including the role of specific forcings such as land use and aerosols, the effect of a peak and decline in forcing, the consequences of scenarios that limit warming to below 2 °C, the relative contributions to uncertainty from scenarios, climate models, and internal variability, and long-term climate system outcomes beyond the 21st century. To serve this wide range of scientific communities and address these questions, a design has been identified consisting of eight alternative 21st century scenarios plus one large initial condition ensemble and a set of long-term extensions, divided into two tiers defined by relative priority. Some of these scenarios will also provide a basis for variants planned to be run in other CMIP6-Endorsed MIPs to investigate questions related to specific forcings. Harmonized, spatially

Development and validation of simple RP-HPLC-PDA analytical protocol for zileuton assisted with Design of Experiments for robustness determination

OpenAIRE

Saurabh B. Ganorkar; Dinesh M. Dhumal; Atul A. Shirkhedkar

2017-01-01

A simple, rapid, sensitive, robust, stability-indicating RP-HPLC-PDA analytical protocol was developed and validated for the analysis of zileuton racemate in bulk and in tablet formulation. Development of method and resolution of degradation products from forced; hydrolytic (acidic, basic, neutral), oxidative, photolytic (acidic, basic, neutral, solid state) and thermal (dry heat) degradation was achieved on a LC – GC Qualisil BDS C18 column (250 mm × 4.6 mm × 5 μm) by isocratic mode at ambie...

Effect of the mobile phase on the RP-HPLC analysis of 99mTc-ECD

International Nuclear Information System (INIS)

Almeida, Erika V.; Monteiro, Elisiane G.; Mengatti, Jair; Fukumori, Neuza T.O.; Silva, Constancia P.G. da; Matsuda, Margareth M.N.

2009-01-01

Technetium-99m labeled L,L-ethylene cysteine dimer ( 99m Tc-ECD) is a neutral, lipophilic complex and brain perfusion imaging agent. The aim of this study was to investigate the effect of the mobile phase on the reversed phase high performance liquid chromatography (RP-HPLC) analysis of 99m Tc-ECD. The HPLC system was LC20AT Prominence model and a Shim-Pack VP-ODS column (250 x 4.6 mm i.d., 5 μm). 99m Tc-ECD was prepared by adding 1 mL of 0.9% NaCl, 1 mL of phosphate buffer (pH 7.5) and 1 mL of Na 99m TcO 4 . The radioactive concentration was 55.5 MBq mL -1 . 20 μL sample volume was injected and 1.0 mL min -1 flow rate was applied. A linear gradient was performed separately with a mixture of ethanol with three different solvents: 12.5 mmol L -1 phosphate buffer (pH 2.5) (solvent A), 0.2 % PIC A (w/v) (pH 6.0) (solvent B) and 0.2 % PIC B5 (w/v) (pH 4.0) (solvent C). 99m Tc-ECD retention times for the mixture of ethanol with solvent A, B and C were 17.38, 17.65 and 17.60 minutes, respectively. These results suggested that the ion pairing reagents (PIC A and PIC B5) did not influence the 99m Tc-ECD analysis, but affected the retention time of impurities as 99m Tc-EC (ethylene dicysteine) and 99m TcO 4 -. 99m Tc-ECD radiochemical purity determined by RP-HPLC was higher than 96% for the mixture of ethanol with the three solvents. The results indicated that the retention time of 99m Tc-ECD was not influenced by the nature of the mobile phase. (author)

New Stability Indicating RP-HPLC Method for the Estimation of Cefpirome Sulphate in Bulk and Pharmaceutical Dosage Forms

OpenAIRE

Rao, Kareti Srinivasa; Kumar, Keshar Nargesh; Joydeep, Datta

2011-01-01

A simple stability indicating reversed-phase HPLC method was developed and subsequently validated for estimation of Cefpirome sulphate (CPS) present in pharmaceutical dosage forms. The proposed RP-HPLC method utilizes a LiChroCART-Lichrosphere100, C18 RP column (250 mm ? 4mm ? 5 ?m) in an isocratic separation mode with mobile phase consisting of methanol and water in the proportion of 50:50 % (v/v), at a flow rate 1ml/min, and the effluent was monitored at 270 nm. The retention time of CPS wa...

Corrosión de componentes de aluminio en el reactor RP-10

OpenAIRE

Morales Larrea, Soledad; Tenorio de la Cruz, Favio

1983-01-01

Se analiza la velocidad de corrosión, por pérdida de peso de placas de aluminio a diversos valores de pH y temperatura en solución acuosa. El estudio se hace simulando las condiciones de trabajo (pH y temperatura) a las que pueden verse sometidas las vainas de aluminio de los elementos combustibles del reactor RP-10.

Twenty years of Radiology in RP-10 nuclear reactor protection

International Nuclear Information System (INIS)

Zapata, Alejandro L.; Ramos, Fernando T.; Arrieta, Rolando W.B.; Vela Mora, Mariano

2013-01-01

In this report we present the results about radiation controls during 1990 - 2010, carried out in the Nuclear Reactor RP-10 of the Nuclear Center of Huarangal. These controls and radiological evaluation are of much utility for the radio personnel protection of this one and other reactors, since it allows to compares these variables with respect to the time. From the results obtained in monitoring and radiation controls, we conclude that in no case it has been reached the limits allowed by the Peruvian Regulating Authority. (author)

Dopamine serotonin stabilizer RP5063: A randomized, double-blind, placebo-controlled multicenter trial of safety and efficacy in exacerbation of schizophrenia or schizoaffective disorder.

Science.gov (United States)

Cantillon, Marc; Prakash, Arul; Alexander, Ajay; Ings, Robert; Sweitzer, Dennis; Bhat, Laxminarayan

2017-11-01

The study objectives were to evaluate the efficacy, safety, tolerability, and pharmacokinetics of RP5063 versus placebo. The study was conducted in adults with acute exacerbation of schizophrenia or schizoaffective disorder. This 28-day, multicenter, placebo-controlled, double-blind study randomized 234 subjects to RP5063 15, 30, or 50mg; aripiprazole; or placebo (3:3:3:1:2) once daily. The aripiprazole arm was included solely to show assay sensitivity and was not powered to show efficacy. The primary endpoint was change from baseline to Day 28/EOT (End-of-Treatment) in Positive and Negative Syndrome Scale (PANSS) total score; secondary endpoints included PANSS subscales, improvement ≥1 point on the Clinical Global Impressions-Severity (CGI-S), depression and cognition scales. The primary analysis of PANSS Total showed improvement by a mean (SE) of -20.23 (2.65), -15.42 (2.04), and -19.21 (2.39) in the RP5063 15, 30, and 50mg arms, versus -11.41 (3.45) in the placebo arm. The difference between treatment and placebo reached statistical significance for the 15mg (p=0.021) and 50mg (p=0.016) arms. Improvement with RP5063 was also seen for multiple secondary efficacy outcomes. Discontinuation for any reason was much lower for RP5063 (14%, 25%, 12%) versus placebo (26%) and aripiprazole (35%). The most common treatment-emergent adverse events (TEAE) in the RP5063 groups were insomnia and agitation. There were no significant changes in body weight, electrocardiogram, or incidence of orthostatic hypotension; there was a decrease in blood glucose, lipid profiles, and prolactin levels. In conclusion, the novel dopamine serotonin stabilizer, RP5063 is an efficacious and well-tolerated treatment for acute exacerbation of schizophrenia or schizoaffective disorder. Copyright © 2017. Published by Elsevier B.V.

$\\beta$-decay half-life of $^{70}$Kr a bridge nuclide for the rp-process beyond A = 70

CERN Document Server

Oinonen, M; Jokinen, A; Baumann, P; Didierjean, François; Huck, A; Knipper, A; Ramdhane, M; Walter, G; Van Duppen, P; Huyse, M; Marguier, G; Novikov, Yu N; Popov, A; Seliverstov, D M; Schatz, H

2000-01-01

The $\\beta$-decay half-life of $^{70}$Kr has been measured for the first time at the ISOLDE PSB Facility at CERN. Mass separated $^{70}$Kr ions were produced by 1 GeV proton induced spallation reactions in a Nb foil. The measured half-life is 57(21) ms. This value is consistent with the half-life calculated assuming a pure Fermi decay, but is clearly lower than the value used in a recent rp-process reaction flow calculation. The result shows that the reaction flow via two-proton-capture of $^{68}$Se is 2.5 times faster than previously calculated assuming an astrophysical temperature of 1.5 GK and a density of 10$^{6}$g/cm$^{3}$.

Improving lactose digestion and symptoms of lactose intolerance with a novel galacto-oligosaccharide (RP-G28): a randomized, double-blind clinical trial.

Science.gov (United States)

Savaiano, Dennis A; Ritter, Andrew J; Klaenhammer, Todd R; James, Gareth M; Longcore, Amy T; Chandler, Justin R; Walker, W Allan; Foyt, Howard L

2013-12-13

Lactose intolerance (LI) is a common medical problem with limited treatment options. The primary symptoms are abdominal pain, diarrhea, bloating, flatulence, and cramping. Limiting dairy foods to reduce symptoms contributes to low calcium intake and the risk for chronic disease. Adaptation of the colon bacteria to effectively metabolize lactose is a novel and potentially useful approach to improve lactose digestion and tolerance. RP-G28 is novel galacto-oligosaccharide (GOS) being investigated to improve lactose digestion and the symptoms of lactose intolerance in affected patients. A randomized, double-blind, parallel group, placebo-controlled study was conducted at 2 sites in the United States. RP-G28 or placebo was administered to 85 patients with LI for 35 days. Post-treatment, subjects reintroduced dairy into their daily diets and were followed for 30 additional days to evaluate lactose digestion as measured by hydrogen production and symptom improvements via a patient-reported symptom assessment instrument. Lactose digestion and symptoms of LI trended toward improvement on RP-G28 at the end of treatment and 30 days post-treatment. A reduction in abdominal pain was also demonstrated in the study results. Fifty percent of RP-G28 subjects with abdominal pain at baseline reported no abdominal pain at the end of treatment and 30 days post treatment (p = 0.0190). RP-G28 subjects were also six times more likely to claim lactose tolerance post-treatment once dairy foods had been re-introduced into their diets (p = 0.0389). Efficacy trends and favorable safety/tolerability findings suggest that RP-G28 appears to be a potentially useful approach for improving lactose digestion and LI symptoms. The concurrent reduction in abdominal pain and improved overall tolerance could be a meaningful benefit to lactose intolerant individuals.

Improving lactose digestion and symptoms of lactose intolerance with a novel galacto-oligosaccharide (RP-G28): a randomized, double-blind clinical trial

Science.gov (United States)

2013-01-01

Background Lactose intolerance (LI) is a common medical problem with limited treatment options. The primary symptoms are abdominal pain, diarrhea, bloating, flatulence, and cramping. Limiting dairy foods to reduce symptoms contributes to low calcium intake and the risk for chronic disease. Adaptation of the colon bacteria to effectively metabolize lactose is a novel and potentially useful approach to improve lactose digestion and tolerance. RP-G28 is novel galacto-oligosaccharide (GOS) being investigated to improve lactose digestion and the symptoms of lactose intolerance in affected patients. Methods A randomized, double-blind, parallel group, placebo-controlled study was conducted at 2 sites in the United States. RP-G28 or placebo was administered to 85 patients with LI for 35 days. Post-treatment, subjects reintroduced dairy into their daily diets and were followed for 30 additional days to evaluate lactose digestion as measured by hydrogen production and symptom improvements via a patient-reported symptom assessment instrument. Results Lactose digestion and symptoms of LI trended toward improvement on RP-G28 at the end of treatment and 30 days post-treatment. A reduction in abdominal pain was also demonstrated in the study results. Fifty percent of RP-G28 subjects with abdominal pain at baseline reported no abdominal pain at the end of treatment and 30 days post treatment (p = 0.0190). RP-G28 subjects were also six times more likely to claim lactose tolerance post-treatment once dairy foods had been re-introduced into their diets (p = 0.0389). Conclusions Efficacy trends and favorable safety/tolerability findings suggest that RP-G28 appears to be a potentially useful approach for improving lactose digestion and LI symptoms. The concurrent reduction in abdominal pain and improved overall tolerance could be a meaningful benefit to lactose intolerant individuals. Study registration ClinicalTrials.gov NCT01113619. PMID:24330605

Genetic heterogeneity and consanguinity lead to a "double hit": homozygous mutations of MYO7A and PDE6B in a patient with retinitis pigmentosa.

Science.gov (United States)

Goldenberg-Cohen, Nitza; Banin, Eyal; Zalzstein, Yael; Cohen, Ben; Rotenstreich, Ygal; Rizel, Leah; Basel-Vanagaite, Lina; Ben-Yosef, Tamar

2013-01-01

Retinitis pigmentosa (RP), the most genetically heterogeneous disorder in humans, actually represents a group of pigmentary retinopathies characterized by night blindness followed by visual-field loss. RP can appear as either syndromic or nonsyndromic. One of the most common forms of syndromic RP is Usher syndrome, characterized by the combination of RP, hearing loss, and vestibular dysfunction. The underlying cause of the appearance of syndromic and nonsyndromic RP in three siblings from a consanguineous Israeli Muslim Arab family was studied with whole-genome homozygosity mapping followed by whole exome sequencing. THE FAMILY WAS FOUND TO SEGREGATE NOVEL MUTATIONS OF TWO DIFFERENT GENES: myosin VIIA (MYO7A), which causes type 1 Usher syndrome, and phosphodiesterase 6B, cyclic guanosine monophosphate-specific, rod, beta (PDE6B), which causes nonsyndromic RP. One affected child was homozygous for both mutations. Since the retinal phenotype seen in this patient results from overlapping pathologies, one might expect to find severe retinal degeneration. Indeed, he was diagnosed with RP based on an abnormal electroretinogram (ERG) at a young age (9 months). However, this early diagnosis may be biased, as two of his older siblings had already been diagnosed, leading to increased awareness. At the age of 32 months, he had relatively good vision with normal visual fields. Further testing of visual function and structure at different ages in the three siblings is needed to determine whether the two RP-causing genes mutated in this youngest sibling confer increased disease severity. This report further supports the genetic heterogeneity of RP, and demonstrates how consanguinity could increase intrafamilial clustering of multiple hereditary diseases. Moreover, this report provides a unique opportunity to study the clinical implications of the coexistence of pathogenic mutations in two RP-causative genes in a human patient.

A Study of the Impacts of Npp-Desalination Development in Madura on Sectoral Regional Economy

International Nuclear Information System (INIS)

Bambang Eko-Afiatno; Mochammad Nasrullah; Sriyana

2006-01-01

This study aims to assess the economic impact of construction and early operation of the nuclear power plant (NPP)-desalination project in the island of Mad Lira until the year 2018. Long-term projection on economic output (X) of Madura uses Leo ntiefdynamic 1-0 (input-output) model, and for GRDP- final demand (Y) uses time series model with random growth adjustment based on autoregressive model. Since the Madura 1-0 Table is not available, then it is necessary to construct it for 2000 using RAS method and some modifications. The NPP project will use SMART technology with 2 units of power generators (100 MWe capacity per unit, total output 200 MWe), but to be built sequentially with one year lag. As for the desalination will use 4 units MED with each unit capacity of 10.000 m'/day. The construction stage will take 5 years to be completed (2014-2018), preceded by the pre-project stage along 2010-2013. Total investment requirements of the project is amounted to US$ 357,87 million (in 2002). At the time when the contract (turn key contract) is signed in 2009, the value will become US$ 397.18 million, and in 2014 (early construction) will be US$ 427.87 million. At the end of the project (2018), total investment requirements will amount to US$ 440.79 million. To include land make up payment and licenses costs the project will be worth US$ 476. In the pre-project stage (2010-2013), cumulatively, land make up payment and licenses management activities as much as Rp 114.11 billion (US$ 10.69 million) has indirect effect--transmiUed through private and government consumption -- onto Madura economy. Dynamic 1-0 simulation results (2000 Madura 1-0 Table, 1Ox10) show that the rise in consumption generate increases in output, GRDP and employment respectively in cumulative as much as Rp 146.39 billion, Rp79.20 billion, and 7,428 men. In overall, project activities in construction stage (2014-2017) estimated to Rp 231.37 billion (US$19.36 million) which has direct effect on

The Determination of Food Dyes in Vitamins by RP-HPLC

Directory of Open Access Journals (Sweden)

Monika Šuleková

2016-10-01

Full Text Available Reversed-phase high performance liquid chromatography (RP-HPLC for the determination of five synthetic food dyes (Quinoline Yellow E104, Sunset Yellow E110, Ponceau 4R E124, Tartrazine E102 and Carmine E120 in vitamins was used. The dyes were analyzed within 10 min using a column with stationary phase C 18 (250 mm × 4.6 mm, 5 μm at 40 °C with isocratic elution, and the mobile phase contained acetonitrile and a mixture of CH3COONa:CH3OH (85:15, v/v in a ratio of 10:90 (v/v for yellow-colored capsules and 20:80 (v/v for red-colored capsules, respectively. A diode-array detector was used to monitor the dyes between 190 and 800 nm. It was established that the analyzed samples contained synthetic dyes in a concentration range from 79.5 ± 0.01 μg/capsule of Ponceau 4R, E124 to 524 ± 0.01 μg/capsule of Tartrazine, E102. The obtained results were compared with existing acceptable daily intakes (ADIs for individual dyes. This paper provides information about the content of dyes in samples of vitamins. This information is not generally available to consumers.

EC FP6 Siberia-focused Enviro-RISKS Project and its Outcomes

Science.gov (United States)

Baklanov, A. A.; Gordov, E. P.

2009-04-01

The FP6 Project "Man-induced Environmental Risks: Monitoring, Management and Remediation of Man-made Changes in Siberia" (Enviro-RISKS) strategic objective is to facilitate elaboration of solid scientific background and understanding of man-made associated environmental risks, their influence on all aspects of regional environment and optimal ways for it remediation by means of coordinated initiatives of a range of relevant RTD projects as well as to achieve their improved integration thus giving the projects additional synergy in current activities and potential for practical applications. List of Partners includes 3 leading European research organizations, 6 leading Russian research organizations (5 - located in Siberia) and 1 organization from Kazakhstan. Additionally several Russian and European research organizations joined to the Project as Associated Partners. Scientific background and foundation for the project performance is formed by a number of different levels RTD projects carried out by Partners and devoted to near all aspects of the theme. The set comprise coordinated/performed by partners EC funded thematic international projects, Russian national projects and other projects performed by NIS partners. Project outcomes include, in particular, development and support of the bilingual Enviro-RISKS web portal (http://risks.scert.ru/) as the major tool for disseminations of environmental information and project results; achieved level of development of Siberia Integrated Regional Study (SIRS, http://sirs.scert.ru/), which is the Siberia-focused NEESPI Environmental Mega-Project ongoing under the auspices of the Siberian Branch of the Russian Academy of Sciences. The main Project outcome are Memorandum on the state of the art of environmental RTD activity in Siberia and Recommendations on future environmental RTD activity in Siberia elaborated by four Working Experts Groups working in most important for Siberia Thematic Focuses. Three Thematic Focuses

Neutral Higgs Sector of the MSSM without $R_{p}$

CERN Document Server

Davidson, Sacha; Rius, N

2000-01-01

We analyse the neutral scalar sector of the MSSM without R-parity. Our analysis is performed for a one-generation model in terms of ``basis-independent'' parameters, and includes one-loop corrections due to large yukawa couplings. We concentrate on the consequences of large $R_p$ violating masses in the soft sector, which mix the Higgses with the sleptons, because these are only constrained by their one-loop contributions to neutrino masses. We focus on the effect of $R_p$-violation on the Higgs mass and branching ratios. We find that the experimental lower bound on the lightest CP-even Higgs in this model can be lower than in the MSSM.

[Positive inotropic and lusitropic effect of RP 62719, a new class III antiarrhythmia agent].

Science.gov (United States)

Beregi, J P; Escande, D; Coudray, N; Chemla, D; Mestre, M; Péry, N; Lecarpentier, Y

1994-02-01

Antiarrhythmic drugs, especially the Class I family, exert a negative inotropic effect on the myocardium which is particularly undesirable in patients with depressed left ventricular function. Therefore, research has been directed to the development of new, more specific molecules of the Class III family. The authors studies the mechanical effects of RP 62719 on guinea pig left ventricular papillary muscle. This new molecule is a pure Class III antiarrhythmic, known to lengthen the duration of the cardiac action potential by selectively blocking the potassium current iK1 (inward rectifier K+ current). The mechanical parameters were determined during the phases of contraction and relaxation under isotonic and isometric conditions. At 0.2 and 2 microM concentrations, RP 62719 improved cardiac contraction under both isotonic and isometric conditions with an increase of about 30% of Vmax (p delta 1 (p force normalized per cross-sectional area [AF/S (p actions could provide significant therapeutic advantages especially in patients cardiac failure.

Genetic heterogeneity and consanguinity lead to a “double hit”: Homozygous mutations of MYO7A and PDE6B in a patient with retinitis pigmentosa

Science.gov (United States)

Goldenberg-Cohen, Nitza; Banin, Eyal; Zalzstein, Yael; Cohen, Ben; Rotenstreich, Ygal; Rizel, Leah; Basel-Vanagaite, Lina

2013-01-01

Purpose Retinitis pigmentosa (RP), the most genetically heterogeneous disorder in humans, actually represents a group of pigmentary retinopathies characterized by night blindness followed by visual-field loss. RP can appear as either syndromic or nonsyndromic. One of the most common forms of syndromic RP is Usher syndrome, characterized by the combination of RP, hearing loss, and vestibular dysfunction. Methods The underlying cause of the appearance of syndromic and nonsyndromic RP in three siblings from a consanguineous Israeli Muslim Arab family was studied with whole-genome homozygosity mapping followed by whole exome sequencing. Results The family was found to segregate novel mutations of two different genes: myosin VIIA (MYO7A), which causes type 1 Usher syndrome, and phosphodiesterase 6B, cyclic guanosine monophosphate-specific, rod, beta (PDE6B), which causes nonsyndromic RP. One affected child was homozygous for both mutations. Since the retinal phenotype seen in this patient results from overlapping pathologies, one might expect to find severe retinal degeneration. Indeed, he was diagnosed with RP based on an abnormal electroretinogram (ERG) at a young age (9 months). However, this early diagnosis may be biased, as two of his older siblings had already been diagnosed, leading to increased awareness. At the age of 32 months, he had relatively good vision with normal visual fields. Further testing of visual function and structure at different ages in the three siblings is needed to determine whether the two RP-causing genes mutated in this youngest sibling confer increased disease severity. Conclusions This report further supports the genetic heterogeneity of RP, and demonstrates how consanguinity could increase intrafamilial clustering of multiple hereditary diseases. Moreover, this report provides a unique opportunity to study the clinical implications of the coexistence of pathogenic mutations in two RP-causative genes in a human patient. PMID:23882135

Utilization of RP-10 reactor for neutron therapy

International Nuclear Information System (INIS)

Paucar, R.; Nieto, M.; Parreno, F.; Vela, M.; Pozo, Z.

1997-01-01

In the Nuclear Energy Peruvian Institute, IPEN, a research area has established of Neutron Radiotherapy, know as NCT. This research joins the physics of particles (Neutrons and photons) and Medical Physics, and this one is an applied investigation where in considering the construction of a treatment hall in Huarangal (Peru) Reactor's irradiation facility, it can treat patients with brain tumors. In Neutron Therapy (NCT), it tries to use neutrons to destroy tumor cells where other therapeutic techniques are not effective. This process consist on to incise a neutrons beam of adequate characteristics over the tumor area of the patient. The neutrons used are of thermal energy and therefore irradiations are developed in experimental reactors. For this one, it is used horizontal channels prepared suitably. Before the irradiation, it is injected to the patient a substance which is absorbed by tumoral tissue. The substance components will be B-10, nuclide with an absorption cross section high to thermal neutrons (3837 b). The B-10 irradiate with thermal neutrons produce alpha particles of short reach (10 μm. on soft tissue) and with LET values (lineal energy transference) very high. The result is a cell preferential destruction which have absorbed the substance and it's next neighbors, like the cell size is 10 μm. This process as know as Boron Neutron Capture Therapy (BNCT). This work describes Peruvian RP-10 reactor and recently efforts to assess the design and feasibility of the medical neutron irradiation facility for NCT. (author). 22 refs., 6 tabs

Investigation of Back-Projection Uncertainties with M6 Earthquakes

Science.gov (United States)

Fan, W.; Shearer, P. M.

2017-12-01

We investigate possible biasing effects of inaccurate timing corrections on teleseismic P-wave back-projection imaging of large earthquake ruptures. These errors occur because empirically-estimated time shifts based on aligning P-wave first arrivals are exact only at the hypocenter and provide approximate corrections for other parts of the rupture. Using the Japan subduction zone as a test region, we analyze 46 M6-7 earthquakes over a ten-year period, including many aftershocks of the 2011 M9 Tohoku earthquake, performing waveform cross-correlation of their initial P-wave arrivals to obtain hypocenter timing corrections to global seismic stations. We then compare back-projection images for each earthquake using its own timing corrections with those obtained using the time corrections for other earthquakes. This provides a measure of how well sub-events can be resolved with back-projection of a large rupture as a function of distance from the hypocenter. Our results show that back-projection is generally very robust and that sub-event location errors average about 20 km across the entire study region ( 700 km). The back-projection coherence loss and location errors do not noticeably converge to zero even when the event pairs are very close (<20 km). This indicates that most of the timing differences are due to 3D structure close to each of the hypocenter regions, which limits the effectiveness of attempts to refine back-projection images using aftershock calibration, at least in this region.

Can (Or Should) RP Serve as the Model for the Teaching of English ...

African Journals Online (AJOL)

This paper presents the case for and against the continued adoption of RP, but argues that the spread of English to many parts of the world should change our perception of what constitutes Standard English – that the speech of a tiny minority in Britain can no longer be considered the norm by which all others must be ...

Könyvtükörben : Paul-Eerik Rummo / Károly Árpás

Index Scriptorium Estoniae

Árpás, Károly

2003-01-01

Luuletõlked tekstis: "hidegtől kék..." ("külmast siniste..."); "Az én dalom te vagy..."; Liivre gondolva mindig 1-2 (Ikka Liivist mõteldes); Epiloog: tempora mutantur (tõlkinud Károly Árpás)

Stability-indicating RP-HPLC method for simultaneous determination of gatifloxacin and flurbiprofen in binary combination

Directory of Open Access Journals (Sweden)

Islam Ullah Khan

2014-04-01

Full Text Available A stability-indicating RP-HPLC method is presented for determination of gatifloxacin and flurbiprofen in binary combination. Gatifloxacin, flurbiprofen and their degradation products were detected at 254 nm using a BDS Hypersil C8 (250 X 4.6 mm, 5 µm column and mixture of 20 mM phosphate buffer (pH 3.0 and methanol 30:70 v/v as mobile phase. Response was linear over the range of 15-105 mg mL-1 for gatifloxacin (r² > 0.998 and of 1.5-10.5 mg mL-1 for flurbiprofen (r² > 0.999. The developed method efficiently separated the analytical peaks from degradation products (peak purity index > 0.9999. The method developed can be applied successfully for determination of gatifloxacin and flurbiprofen in human serum, urine, pharmaceutical formulations, and their stability studies.

Conditional Viral Tract Tracing Delineates the Projections of the Distinct Kisspeptin Neuron Populations to Gonadotropin-Releasing Hormone (GnRH) Neurons in the Mouse.

Science.gov (United States)

Yip, Siew Hoong; Boehm, Ulrich; Herbison, Allan E; Campbell, Rebecca E

2015-07-01

Kisspeptin neurons play an essential role in the regulation of fertility through direct regulation of the GnRH neurons. However, the relative contributions of the two functionally distinct kisspeptin neuron subpopulations to this critical regulation are not fully understood. Here we analyzed the specific projection patterns of kisspeptin neurons originating from either the rostral periventricular nucleus of the third ventricle (RP3V) or the arcuate nucleus (ARN) using a cell-specific, viral-mediated tract-tracing approach. We stereotaxically injected a Cre-dependent recombinant adenovirus encoding farnesylated enhanced green fluorescent protein into the ARN or RP3V of adult male and female mice expressing Cre recombinase in kisspeptin neurons. Fibers from ARN kisspeptin neurons projected widely; however, we did not find any evidence for direct contact with GnRH neuron somata or proximal dendrites in either sex. In contrast, we identified RP3V kisspeptin fibers in close contact with GnRH neuron somata and dendrites in both sexes. Fibers originating from both the RP3V and ARN were observed in close contact with distal GnRH neuron processes in the ARN and in the lateral and internal aspects of the median eminence. Furthermore, GnRH nerve terminals were found in close contact with the proximal dendrites of ARN kisspeptin neurons in the ARN, and ARN kisspeptin fibers were found contacting RP3V kisspeptin neurons in both sexes. Together these data delineate selective zones of kisspeptin neuron inputs to GnRH neurons and demonstrate complex interconnections between the distinct kisspeptin populations and GnRH neurons.

Erythroblast differentiation at spleen in Q137E mutant ribosomal protein S19 gene knock-in C57BL/6J mice.

Science.gov (United States)

Yamanegi, Koji; Yamada, Naoko; Nakasho, Keiji; Nishiura, Hiroshi

2018-01-01

We recently found that erythroblast-like cells derived from human leukaemia K562 cells express C5a receptor (C5aR) and produce its antagonistic and agonistic ligand ribosomal protein S19 (RP S19) polymer, which is cross-linked between K122 and Q137 by tissue transglutaminases. RP S19 polymer binds to the reciprocal C5aRs on erythroblast-like cells and macrophage-like cells derived from human monocytic THP-1 cells and promotes differentiation into reticulocyte-like cells through enucleation in vitro. To examine the roles of RP S19 polymer in mouse erythropoiesis, we prepared Q137E mutant RP S19 gene knock-in C57BL/6J mice. In contrast to wild-type mice, erythroblast numbers at the preliminary stage (CD71 high /TER119 low ) in spleen based on transferrin receptor (CD71) and glycophorin A (TER119) values and erythrocyte numbers in orbital artery bloods were not largely changed in knock-in mice. Conversely, erythroblast numbers at the early stage (CD71 high /TER119 high ) were significantly decreased in spleen by knock-in mice. The reduction of early erythroblast numbers in spleen was enhanced by the phenylhydrazine-induced pernicious anemia model knock-in mice and was rescued by a functional analogue of RP S19 dimer S-tagged C5a/RP S19. These data indicated that RP S19 polymer plays the roles in the early erythroblast differentiation of C57BL/6J mouse spleen. Copyright © 2017 Elsevier GmbH. All rights reserved.

Interaction of HSP20 with a viral RdRp changes its sub-cellular localization and distribution pattern in plants.

Science.gov (United States)

Li, Jing; Xiang, Cong-Ying; Yang, Jian; Chen, Jian-Ping; Zhang, Heng-Mu

2015-09-11

Small heat shock proteins (sHSPs) perform a fundamental role in protecting cells against a wide array of stresses but their biological function during viral infection remains unknown. Rice stripe virus (RSV) causes a severe disease of rice in Eastern Asia. OsHSP20 and its homologue (NbHSP20) were used as baits in yeast two-hybrid (YTH) assays to screen an RSV cDNA library and were found to interact with the viral RNA-dependent RNA polymerase (RdRp) of RSV. Interactions were confirmed by pull-down and BiFC assays. Further analysis showed that the N-terminus (residues 1-296) of the RdRp was crucial for the interaction between the HSP20s and viral RdRp and responsible for the alteration of the sub-cellular localization and distribution pattern of HSP20s in protoplasts of rice and epidermal cells of Nicotiana benthamiana. This is the first report that a plant virus or a viral protein alters the expression pattern or sub-cellular distribution of sHSPs.

Medical implants by using RP and investment casting technologies

Directory of Open Access Journals (Sweden)

Milan Horacek

2011-02-01

Full Text Available The paper deals with the production technology of knee joint replacement by using rapid prototyping technology. The aim of the work is to outline the manufacturing technology intended for prototype production with the use of rapid prototyping and investment casting technology for use in orthopaedics and the surgery of knee joint replacement. The research results should make an effective contribution in the attempts to minimize the invasive surgical procedure, shorten the production of knee joint replacement as well as reduce the cost. At present, the research is focused on the preparation of STL data from CT (Computed Tomography and verification of the production technology of prototypes made using available RP technology and its evaluation.

Transcallosal Projections Require Glycoprotein M6-Dependent Neurite Growth and Guidance.

Science.gov (United States)

Mita, Sakura; de Monasterio-Schrader, Patricia; Fünfschilling, Ursula; Kawasaki, Takahiko; Mizuno, Hidenobu; Iwasato, Takuji; Nave, Klaus-Armin; Werner, Hauke B; Hirata, Tatsumi

2015-11-01

The function of mature neurons critically relies on the developmental outgrowth and projection of their cellular processes. It has long been postulated that the neuronal glycoproteins M6a and M6b are involved in axon growth because these four-transmembrane domain-proteins of the proteolipid protein family are highly enriched on growth cones, but in vivo evidence has been lacking. Here, we report that the function of M6 proteins is required for normal axonal extension and guidance in vivo. In mice lacking both M6a and M6b, a severe hypoplasia of axon tracts was manifested. Most strikingly, the corpus callosum was reduced in thickness despite normal densities of cortical projection neurons. In single neuron tracing, many axons appeared shorter and disorganized in the double-mutant cortex, and some of them were even misdirected laterally toward the subcortex. Probst bundles were not observed. Upon culturing, double-mutant cortical and cerebellar neurons displayed impaired neurite outgrowth, indicating a cell-intrinsic function of M6 proteins. A rescue experiment showed that the intracellular loop of M6a is essential for the support of neurite extension. We propose that M6 proteins are required for proper extension and guidance of callosal axons that follow one of the most complex trajectories in the mammalian nervous system. © The Author 2014. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

Projection radiography. Chapter 6

Energy Technology Data Exchange (ETDEWEB)

Poletti, J. L. [UNITEC Institute of Technology, Auckland (New Zealand)

2014-09-15

In its simplest form, X ray imaging is the collection of attenuation shadows that are projected from an ideal X ray point source on to an image receptor. This simple form is true for all X ray imaging modalities, including complex ones that involve source and receptor movement, such as computed tomography (CT). This simplified view, however, is made vastly more complex by the non-ideal point source, by the consequences of projecting a 3-D object on to a 2-D detector and by the presence of scattered radiation, generated within the patient, which will degrade any image that is captured.

Corrosion behavior of cast Ti-6Al-4V alloyed with Cu.

Science.gov (United States)

Koike, Marie; Cai, Zhuo; Oda, Yutaka; Hattori, Masayuki; Fujii, Hiroyuki; Okabe, Toru

2005-05-01

It has recently been found that alloying with copper improved the inherently poor grindability and wear resistance of titanium. This study characterized the corrosion behavior of cast Ti-6Al-4V alloyed with copper. Alloys (0.9 or 3.5 mass % Cu) were cast with the use of a magnesia-based investment in a centrifugal casting machine. Three specimen surfaces were tested: ground, sandblasted, and as cast. Commercially pure titanium and Ti-6Al-4V served as controls. Open-circuit potential measurement, linear polarization, and potentiodynamic cathodic polarization were performed in aerated (air + 10% CO(2)) modified Tani-Zucchi synthetic saliva at 37 degrees C. Potentiodynamic anodic polarization was conducted in the same medium deaerated by N(2) + 10% CO(2). Polarization resistance (R(p)), Tafel slopes, and corrosion current density (I(corr)) were determined. A passive region occurred for the alloy specimens with ground and sandblasted surfaces, as for CP Ti. However, no passivation was observed on the as-cast alloys or on CP Ti. There were significant differences among all metals tested for R(p) and I(corr) and significantly higher R(p) and lower I(corr) values for CP Ti compared to Ti-6Al-4V or the alloys with Cu. Alloying up to 3.5 mass % Cu to Ti-6Al-4V did not change the corrosion behavior. Specimens with ground or sandblasted surfaces were superior to specimens with as-cast surfaces. (c) 2005 Wiley Periodicals, Inc.

RP-HPLC method for simultaneous estimation of vigabatrin, gamma-aminobutyric acid and taurine in biological samples.

Science.gov (United States)

Police, Anitha; Shankar, Vijay Kumar; Narasimha Murthy, S

2018-02-15

Vigabatrin is used as first line drug in treatment of infantile spasms for its potential benefit overweighing risk of causing permanent peripheral visual field defects and retinal damage. Chronic administration of vigabatrin in rats has demonstrated these ocular events are result of GABA accumulation and depletion of taurine levels in retinal tissues. In vigabatrin clinical studies taurine plasma level is considered as biomarker for studying structure and function of retina. The analytical method is essential to monitor taurine levels along with vigabatrin and GABA. A RP-HPLC method has been developed and validated for simultaneous estimation of vigabatrin, GABA and taurine using surrogate matrix. Analytes were extracted from human plasma, rat plasma, retina and brain by simple protein precipitation method and derivatized by naphthalene 2, 3‑dicarboxaldehyde to produce stable fluorescent active isoindole derivatives. The chromatographic analysis was performed on Zorbax Eclipse AAA column using gradient elution profile and eluent was monitored using fluorescence detector. A linear plot of calibration curve was observed in concentration range of 64.6 to 6458, 51.5 to 5150 and 62.5 to 6258 ng/mL for vigabatrin, GABA and taurine, respectively with r 2  ≥ 0.997 for all analytes. The method was successfully applied for estimating levels of vigabatrin and its modulator effect on GABA and taurine levels in rat plasma, brain and retinal tissue. This RP-HPLC method can be applied in clinical and preclinical studies to explore the effect of taurine deficiency and to investigate novel approaches for alleviating vigabatrin induced ocular toxicity. Copyright © 2018. Published by Elsevier B.V.

Bone infected/inflamed conditions: Clinical experience with RP517, a Tc99m-leukotriene B4 receptor antagonist

International Nuclear Information System (INIS)

Soroa, V.; Nicolini, J.; Camin, L.; Ughetti, R.; Velasquez, M.

2003-01-01

Full text: Objectives: Tc 99m- RP517- is a receptor antagonist of Leukotriene B4 (LTB4) labelling predominantly neutrophils, in whole blood. The aim of this clinical trial was to obtain the best acquisition protocol and performance of this agent in detecting bone, joint and soft tissue infected / inflamed pathologies. Methods: RP517 was labelled with 15-20 mCi (555-740MBq) Tc99m per doses. Twenty-six patients studied (F 14, M 12) with range ages 19- 93, suspected of bone, joint and soft tissue infection (25) and one with fever of unknown origin (FUO). Laboratory data, anatomical images and one other scintiscan (MDP, Ga-67 or Tc99m- ciprofloxacin) were evaluated. Acquisition protocol: flow, 30 min, 1, 2, 3, 4 - 24h planar or Spect images as required. Results documented with histology, cultures or clinical follow up. Scans were classified as positive when abnormal uptake persisted in the 24h images or negative when positivity faded, or not detected in later images. Results: Labeling efficiency 86-90%. No adverse reactions encountered. Image protocol modified to a flow, 30 min, 4 and 24h images. Ten-fifteen % of in vivo leucocyte labelling at 1h. Early bile and intestinal excretion did not improve with cleansing enemas or food restrain. Late bone marrow activity is less visible than with in vitro leucocyte labeling. Only 16 patients had proven biopsies and cultures with coincident images in 8 True Positive; other 6 cases were True Negative, 2 False Negative (with positive Ga-67 and Tc99m-ciprofloxacin) and no False Positive. Sensitivity 80%, Specificity 100%. Conclusions: A kit should be produced in order to make it easily available. Pharmaceutically manipulation altering the excretion route could validate studies in abdominal infections and FUO that are now disesteemed. Protocol images must include 24h scans. Advantage of RP517 is the lack of cell labeling manipulation. Larger series must be studied to obtain statistics on joint bone and soft tissue infection

Aggregation of Ribosomal Protein S6 at Nucleolus Is Cell Cycle-Controlled and Its Function in Pre-rRNA Processing Is Phosphorylation Dependent.

Science.gov (United States)

Zhang, Duo; Chen, Hui-Peng; Duan, Hai-Feng; Gao, Li-Hua; Shao, Yong; Chen, Ke-Yan; Wang, You-Liang; Lan, Feng-Hua; Hu, Xian-Wen

2016-07-01

Ribosomal protein S6 (rpS6) has long been regarded as one of the primary r-proteins that functions in the early stage of 40S subunit assembly, but its actual role is still obscure. The correct forming of 18S rRNA is a key step in the nuclear synthesis of 40S subunit. In this study, we demonstrate that rpS6 participates in the processing of 30S pre-rRNA to 18S rRNA only when its C-terminal five serines are phosphorylated, however, the process of entering the nucleus and then targeting the nucleolus does not dependent its phosphorylation. Remarkably, we also find that the aggregation of rpS6 at the nucleolus correlates to the phasing of cell cycle, beginning to concentrate in the nucleolus at later S phase and disaggregate at M phase. J. Cell. Biochem. 117: 1649-1657, 2016. © 2015 Wiley Periodicals, Inc. © 2015 Wiley Periodicals, Inc.

Argentine Radiation Protection Society Experience in RP education and training; Experiencia de la Sociedad Argentina de Radioproteccion en capacitacion en Proteccion Radiologica

Energy Technology Data Exchange (ETDEWEB)

Bomben, A. M.; Ciallella, N. R.; Thomasz, E.; Rudelli, M.; Gisone, P.; Ventura, M.; Gomez Parada, I.; Signoretta, C.

2003-07-01

Since its creation in 1967, the Argentine Radiation Protection Society (SAR) promotes all the radiation protection and nuclear safety aspects not only within the scientific, technical and academic areas, but also to general public. To fulfill this objective, SAR organised training and refresher courses, seminars and workshops on RP subjects. During 2002, SAR organised 7 basic and specialized courses regarding the uses of radioactive materials in industrial applications and the course on medical response in radiological accidents, that was attended by Argentine and other Latin American participants. The programmes of the courses are developed in compliance with the legal requirements and also considering specifics needs. In this paper, the characteristics of the courses are enunciated and basic statistics regarding courses and participants are presented. For the 2003 and 2004, SAR foresees the organisation of 18 courses per year and has the capacity to deliver other courses by request. all the courses are delivered in Spanish language. Based on this educational experience SAR consider a priority the inclusion, of a RP module in all the scientific graduate programmes to generate awareness on the importance of RP. Taking into account the migration of professionals to Europe and North America and the Globalization, SAR advocates the harmonization of RP syllabus to attain an international recognition. (Author)

Comparative evaluation of electrostatic repulsion-hydrophilic interaction chromatography (ERLIC) and high-pH reversed phase (Hp-RP) chromatography in profiling of rat kidney proteome.

Science.gov (United States)

Hao, Piliang; Ren, Yan; Dutta, Bamaprasad; Sze, Siu Kwan

2013-04-26

ERLIC and high-pH RP (Hp-RP) have been reported to be promising alternatives to strong cation exchange (SCX) in proteome fractionation. Here we compared the performance of ERLIC, concatenated ERLIC and concatenated Hp-RP in proteome profiling. The protein identification is comparable in these three strategies, but significantly more unique peptides are identified by the two concatenation methods, resulting in a significant increase of the average protein sequence coverage. The pooling of fractions from spaced intervals results in more uniform distribution of peptides in each fraction compared with the chromatogram-based pooling of adjacent fractions. ERLIC fractionates peptides according to their pI and GRAVY values. These properties remains but becomes less remarkable in concatenated ERLIC. In contrast, the average pI and GRAVY values of the peptides are comparable in each fraction in concatenated Hp-RP. ERLIC performs the best in identifying peptides with pI>9 among the three strategies, while concatenated Hp-RP is good at identifying peptides with pI<4. These advantages are useful when either basic or acidic peptides/proteins are analytical targets. The power of ERLIC in identification of basic peptides seems to be due to their efficient separation from acidic peptides. This study facilitates the choice of proper fractionation strategies based on specific objectives. For in-depth proteomic analysis of a cell, tissue and plasma, multidimensional liquid chromatography (MDLC) is still necessary to reduce sample complexity for improving analytical dynamic range and proteome coverage. This work conducts a direct comparison of three promising first-dimensional proteome fractionation methods. They are comparable in identifying proteins, but concatenated ERLIC and concatenated Hp-RP identify significantly more unique peptides than ERLIC. ERLIC is good at analyzing basic peptides, while concatenated Hp-RP performs the best in analyzing acidic peptides with pI<4. This

[Determination of content and entrapment efficiency of 20 (S)-protopanaxadiol in pharmacosomes by RP-HPLC method].

Science.gov (United States)

Han, Meihua; Chen, Jing; Chen, Shilin; Wang, Xiangtao

2009-05-01

To establish a RP-HPLC method for content and entrapment efficiency of 20 (S)-protopanaxadiol in pharmacosomes. The separation was performed with a COSMOSIL 5 C18-MS-II column (4.6 mm x 250 mm, 5 mmicrom) using methanol-water (95:5) as the mobile phase and detected at 203 nm. The flow rate was 1.0 mL x min(-1) and 50 microL sample solution was injected for each time. The calibration curve was linear within the range 0.1-0.5 mg x mL(-1) (r = 0. 9999) , the intra-day RSD and inter-day RSD were less than 2% and the average recovery was between 101.44%-103.11% (n = 3). The method is simple, accurate, sensitive and applicable for determination of content and entrapment efficiency of 20 (S)-protopanaxadiol pharmacosomes.

Splitter magnets for DAΦNE project

International Nuclear Information System (INIS)

Sanelli, C.; Hsieh, H.

1992-01-01

A 510 MeV electron positron colliding beam facility is under design and construction. The project consists of two storage rings, accumulator, electron/positron linac and transfer lines. The design of the splitter magnets which separate the circulating beams immediately after passing through the DAΦNE interaction point is presented. The results of 2-D and 3-D magnetic calculations is presented, and the electrical and mechanical design of the magnet are described. A 1/3 length prototype of this magnet is under construction. (R.P.) 2 refs.; 8 figs.; 2 tabs

Development and validation of RP-UHPLC procedure for estimation of 5-amino salicyclic acid in 5-amino salicyclic acid rectal suppositories

Science.gov (United States)

Balaji, Jayagopal; Shivashankar, Murugesh

2017-11-01

The present study describes a simple and robust reverse phase ultra performance liquid chromatography (RP-UPLC) method for the quantification of 5-amino salicyclic acid in 5-amino salicyclic acid rectal capsules. Successful separation of Mesalamine peak from excipient peaks and diluent were achieved on a Acquity C8 (50 × 2.1 mm, 1.7 μm) and UV detector at 254 nm, 0.3 mL/min as a flow rate, and 3 μL as an injection volume. For the RP-UPLC method, phosphate buffer and methanol was used as mobile phases at ratio of 83:17 and the column temperature was 25 °C. Percentage recovery obtained in the range of 98.7 - 99.7 % and the method is linear for Mesalamine for specified concentration range with coefficient of variation (r) not less than 0.99. The proposed RP-UPLC method was found to be specific, linear, precise, accurate and robust.

X-ray phase radiography and tomography with grating interferometry and the reverse projection technique

International Nuclear Information System (INIS)

Wang, Zhili; Gao, Kun; Ge, Xin; Wu, Zhao; Chen, Heng; Wang, Shenghao; Wu, Ziyu; Zhu, Peiping; Yuan, Qingxi; Huang, Wanxia; Zhang, Kai

2013-01-01

X-ray grating interferometry provides substantially increased contrast over conventional absorption-based imaging methods, and therefore new and complementary information. Compared with other phase-contrast imaging techniques, x-ray grating interferometry can overcome some of the problems that have impaired the applications of x-ray phase-contrast radiography and phase tomography. Recently, special attention has been paid to the development of quantitative phase retrieval methods, which is mandatory to perform x-ray phase tomography, to achieve material identification, to differentiate distinct tissues, etc. Typically, the phase-stepping approach has been utilized for phase retrieval in grating interferometry. This method requires a grating scanning and acquisition of multiple radiographic projections, and therefore is disadvantageous in terms of imaging speed and radiation damage. Here we present an innovative, highly sensitive approach, dubbed ‘reverse projection’ (RP), for quantitative phase retrieval. Compared with the phase-stepping approach, the present RP method abandons grating scanning completely, and thus is advantageous due to its much higher efficiency and the reduced radiation dose, without the degradation of reconstruction quality. This review presents a detailed explanation of the principle of the RP method. Both radiography and phase tomography experiments are performed to validate the RP method. We believe that this new technique will find widespread applications in biomedical imaging and in vivo studies. (paper)

What Comes Next After a Successful Synthesis of a RP entity? USA Prospective

International Nuclear Information System (INIS)

Dence, Carmen S.

2016-01-01

The successful synthesis of a new RP is the first step - although a critical one- to what comes next: to make it available to a patient. This presentation will present an overview of the regulatory process in USA and how to blend the preclinical to translational research. The latter, then may become amenable to the much discussed 'personalized medicine', one of the goals for patient treatment. (author)

As Built Verification Plan for Cask Transportation Facility Modifications (CTFM) - Project A.5 and A.6

International Nuclear Information System (INIS)

LANE, K.I.

2000-01-01

This document establishes an As-built Verification Plan (AVP) for implementing requirements in PHMC Engineering Requirements HNF-PRO-1819, Rev. 4, Sections 2.8.3.d and 2.10.8 and Spent Nuclear Fuels (SNF) Project Administrative Procedure EN-6-012-01. This AVP defines and implements approved processes to document the physical configuration of the project scope installed within the facility and identify discrepancies between the associated project engineering drawings and the field configuration, and the component index (CI) database as defined in AP EN 6-005-02. This AVP defines requirements for project activities verifying conformance of structures, systems, and components (SSCs) to project specified requirements

Rapid diagnostic detection of plum pox virus in Prunus plants by isothermal AmplifyRP(®) using reverse transcription-recombinase polymerase amplification.

Science.gov (United States)

Zhang, Shulu; Ravelonandro, Michel; Russell, Paul; McOwen, Nathan; Briard, Pascal; Bohannon, Seven; Vrient, Albert

2014-10-01

Plum pox virus (PPV) causes the most destructive viral disease known as plum pox or Sharka disease in stone fruit trees. As an important regulated pathogen, detection of PPV is thus of critical importance to quarantine and eradication of the spreading disease. In this study, the innovative development of two AmplifyRP(®) tests is reported for a rapid isothermal detection of PPV using reverse transcription-recombinase polymerase amplification. In an AmplifyRP(®) test, all specific recombination and amplification reactions occur at a constant temperature without thermal cycling and the test results are either recorded in real-time with a portable fluorescence reader or displayed using a lateral flow strip contained inside an amplicon detection chamber. The major improvement of this assay is that the entire test from sample preparation to result can be completed in as little as 20min and can be performed easily both in laboratories and in the field. The results from this study demonstrated the ability of the AmplifyRP(®) technique to detect all nine PPV strains (An, C, CR, D, EA, M, Rec, T, or W). Among the economic benefits to pathogen surveys is the higher sensitivity of the AmplifyRP(®) to detect PPV when compared to the conventional ELISA and ImmunoStrip(®) assays. This is the first report describing the use of such an innovative technique to detect rapidly plant viruses affecting perennial crops. Copyright © 2014 Elsevier B.V. All rights reserved.

Stability indicating RP-HPLC method for simultaneous determination of piroxicam and ofloxacin in binary combination.

Science.gov (United States)

John, Peter; Azeem, Waqar; Ashfaq, Muhammad; Khan, Islam Ullah; Razzaq, Syed Naeem

2015-09-01

A simple and precise RP-HPLC method was developed for simultaneous determination of piroxicam and ofloxacin in pharmaceutical formulations and human serum. Optimum separations of piroxicam, ofloxacin and stress-induced degradation products were achieved by use of Hypersil BDS C8 column (250 x 4.6mm, 5 μm). The mobile phase was a mixture of acetonitrile: 0.012M K2HPO4: 0.008M sodium citrate (both buffers mixed and pH adjusted to 2.8) (50:25:25 v/v/v) delivered at flow rate of 1.5 mL min⁻¹ using DAD at 254 nm. Response was linear function of concentration over the ranges of 70-130 mg mL⁻¹ for piroxicam and ofloxacin (r² ≥ 0.999). The method efficiently separated the analytical peaks from degradation products with acceptable tailing and resolution. The developed method was successfully used for concurrent analysis of piroxicam and ofloxacin in pharmaceutical formulations, human serum and in vitro drug interaction studies.

Intracerebroventricular C75 decreases meal frequency and reduces AgRP gene expression in rats.

Science.gov (United States)

Aja, Susan; Bi, Sheng; Knipp, Susan B; McFadden, Jill M; Ronnett, Gabriele V; Kuhajda, Francis P; Moran, Timothy H

2006-07-01

3-Carboxy-4-alkyl-2-methylenebutyrolactone (C75), an inhibitor of fatty acid synthase and stimulator of carnitine palmitoyltransferase-1, reduces food intake and body weight in rodents when given systemically or centrally. Intracellular molecular mechanisms involving changes in cellular energy status are proposed to initiate the feeding and body weight reductions. However, effectors that lie downstream of these initial steps are not yet fully identified. Present experiments characterize the time courses of hypophagia and weight loss after single injections of C75 into the lateral cerebroventicle in rats and go on to identify specific meal pattern changes and coinciding alterations in gene expression for feeding-related hypothalamic neuropeptides. C75 reduced chow intake and body weight dose dependently. Although the principal effects occurred on the first day, weight losses relative to vehicle control were maintained over multiple days. C75 did not affect generalized locomotor activity. C75 began to reduce feeding after a 6-h delay. The hypophagia was due primarily to decreased meal number during 6-12 h without a significant effect on meal size, suggesting that central C75 reduced the drive to initiate meals. C75 prevented the anticipated hypophagia-induced increases in mRNA for AgRP in the arcuate nucleus at 22 h and at 6 h when C75 begins to suppress feeding. Overall, the data suggest that gene expression changes leading to altered melanocortin signaling are important for the hypophagic response to intracerebroventricular C75.

Simultaneous Determination of Florfenicol and Diclazuril in Compound Powder by RP-HPLC-UV Method

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Leilei Guo

2014-01-01

Full Text Available A RP-HPLC-UV method was developed and validated for simultaneous determination of florfenicol and diclazuril in compound powder. The separation involved using a SinoChoom ODS-BP C18 (5 μm, 4.6 mm × 250 mm analytical column. The mobile phase was a mixture of acetonitrile-0.2% phosphoric acid (pH was adjusted to 3.0 with triethylamine. The ratio of acetonitrile and 0.2% phosphoric acid in the mobile phase was 60 : 40 (v/v from 0 minutes to 6 minutes and 70 : 30 (v/v from 6.1 minutes to 15 minutes. The flow rate was 1 mL/min. The temperature of the analytical column was maintained at 30°C. The detection was monitored at 225 nm and 277 nm for florfenicol and diclazuril, respectively. The excipients in the compound powder did not interfere with the drug peaks. The calibration curves of florfenicol and diclazuril were fairly linear over the concentration ranges between 50.0–500.0 μg/mL (r=0.9995 and 10.0–100.0 μg/mL (r=0.9992, respectively. The RSD of both the intraday and interday variations was below 2.1% for florfenicol and diclazuril. The method was successfully validated according to International Conference on Harmonisation and proved to be suitable for the simultaneous determination of florfenicol and diclazuril in compound powder.

Prevalence of Amblyopia and Strabismus in White and African-American Children Aged 6 through 71 Months: The Baltimore Pediatric Eye Disease Study

Science.gov (United States)

Friedman, David S.; Repka, Michael X.; Katz, Joanne; Giordano, Lydia; Ibironke, Josephine; Hawse, Patricia; Tielsch, James M.

2009-01-01

Objective To determine the age-specific prevalence of strabismus in White and African-American children aged 6 through 71 months and of amblyopia in White and African-American children aged 30 through 71 months. Design Cross-sectional population-based study. Participants White and African-American children aged 6 through 71 months in Baltimore, Maryland, United States. Among 4,132 children identified, 3,990 eligible children (97%) were enrolled and 2,546 children (62%) were examined. Methods Parents or guardians of eligible participants underwent an in-home interview and were scheduled for a detailed eye examination including optotype visual acuity and measurement of ocular deviations. Strabismus was defined as a heterotropia at near or distance fixation. Amblyopia was assessed in those children aged 30 through 71 months who were able to perform optotype testing at 3 meters. Main Outcome Measures The proportions of children aged 6 through 71 months with strabismus and of children aged 30 through 71 months with amblyopia. Results Manifest strabismus was found in 3.3% of White and 2.1% of African American children (relative prevalence (RP) 1.61, 95% confidence interval (CI): 0.97, 2.66). Esotropia and exotropia each accounted for close to half of all strabismus in both groups. Only one case of strabismus was found among 84 White children 6 through 11 months of age. Rates were higher in children 60 through 71 months of age (5.8% for Whites and 2.9% for African Americans (RP: 2.05, 95% CI: 0.79, 5.27). Amblyopia was present in 12 (1.8%) White and 7 (0.8%) African American children (RP: 2.23, 95% CI: 0.88, 5.62). Only one child had bilateral amblyopia. Conclusions Manifest strabismus affected 1 in 30 White and 1 in 47 African-American preschool aged children. The prevalence of amblyopia was under 2% in both Whites and African-Americans. National population projections suggest that there are approximately 677,000 cases of manifest strabismus among children 6–71 months

Süt Proteinlerinin RP-HPLC ile Saptanması (İngilizce

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Zerrin Yüksel

2015-02-01

Full Text Available Bu çalışmada, κ-, α- and β-kazein ile α-laktalbumin and β-laktoglobulin, bir RP kolonunun kullanıldığı HPLC-UV ile basit ve hızlı bir yöntem geliştirilerek saptanmıştır. Gradient elüsyonu, 1 ml/dk akış hızında ve 25 oC sıcaklıkta iki çözücü karışımı kullanılarak yürütülmüştür. A çözücüsü asetonitril: su:trifloroasetik asit (100:900:1 ve B çözücüsü asetonitril: su: trifloroasetik asit (100:900:1 karışımlarından oluşmaktadır. Akış, bir UV dedektörü kullanılarak 220 nm’de kaydedilmiştir. Farklı yöntemler kullanılarak RP-HPLC analizi için hazırlanan örneklerde, süt proteinlerine ait farklı kromatografik profiller elde edilmiştir. Diğer örnek hazırlama yöntemlerine kıyasla, enjeksiyondan önce belirtilen oranlarda A ve B çözücülerinin kullanıldığı örnek hazırlama yöntemi ile separasyonun daha keskin ve efektif olduğu ortaya konulmuştur. Bu çalışmada ortaya konulan örnek hazırlama tekniği ve elusyon pratiği ile, süt proteinlerinin analizi basit, hızlı ve duyarlı bir şekilde gerçekleştirilmiştir.

[Analysis of phenylethanoid glycosides of Herba cistanchis by RP-HPLC].

Science.gov (United States)

Tu, P F; Wang, B; Deyama, T; Zhang, Z G; Lou, Z C

1997-04-01

The Chinese drug "Rou Cong-rong" (Herba Cistanchis) is one of the commonly used drugs in Chinese traditional medicine. It is used to reinforce the vital function of kidney, especially that of the sexual organs and induce laxation, for the treatment of impotence, premature ejaculation in men, infertility, morbid leukorrhea, profuse metrorrhagia in women, and chronic constipation in the aged. This paper deals with the qualitative and quantitative analysis of phenylethanoid glycosides of four species and one variety of Genus Cistanche and 23 lots of commercial crude drugs of Herba Cistanchis by RP-HPLC. The results were as follows: the chemical constituents of Cistanche deserticola Ma, C. salsa (C. A. Mey) G. Beck, C. salsa var. albiflora P. F. Tu et Z. C. Lou and C. tubulosa were similar while those of C. sinensis were different from the others; the contents of echinacoside and acteoside of C. salsa, which were 2.13% and 1.51%, were the highest of the genus Cistanche. An ODS column (Alltima C18, 5 microns, 250 x 4.6 mm) was employed. Linear gradient elution of acetonitrile--1.5% acetic acid was used as mobile phase, and concentration of acetontrile was from 8% to 20% (0-60 min) in the qualitative analysis, and from 11.5 to 20% (0-35 min) in the quantitative analysis. The flow rate was 1.2 ml.min-1. The detection wavelength was set at 335 nm.

Criticality calculation of the deposits for the fuel elements in RP-10 nuclear research reactor

International Nuclear Information System (INIS)

Aguirre, Alvaro; Bruna, Ruben

2013-01-01

This paper shows the results of the criticality calculation of the deposits for irradiated and non-irradiated fuel elements in the RP-10 research reactor with MCNP5 code. In all cases and for normal and incidental conditions, the effective multiplication factor (K eff ) results less than 0,90 according to the acceptance criterion. (authors).

The role of GluN2A and GluN2B NMDA receptor subunits in AgRP and POMC neurons on body weight and glucose homeostasis

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Aykut Üner

2015-10-01

Conclusions: GluN2B-containing NMDA receptors in AgRP neurons play a critical role in central control of body weight homeostasis and blood glucose balance via mechanisms that likely involve regulation of AgRP neuronal survival and structure, and modulation of hypothalamic leptin action.

Simplified RP-HPLC method for multi-residue analysis of abamectin, emamectin benzoate and ivermectin in rice.

Science.gov (United States)

Xie, Xianchuan; Gong, Shu; Wang, Xiaorong; Wu, Yinxing; Zhao, Li

2011-01-01

A rapid, reliable and sensitive reverse-phase high-performance liquid chromatography method with fluorescence detection (RP-FLD-HPLC) was developed and validated for simultaneous analysis of the abamectin (ABA), emamectin (EMA) benzoate and ivermectin (IVM) residues in rice. After extraction with acetonitrile/water (2 : 1) with sonication, the avermectin (AVMs) residues were directly derivatised by N-methylimidazole (N-NMIM) and trifluoroacetic anhydride (TFAA) and then analysed on RP-FLD-HPLC. A good linear relationship (r(2 )> 0.99) was obtained for three AVMs ranging from 0.01 to 5 microg ml(-1), i.e. 0.01-5.0 microg g(-1) in rice matrix. The limit of detection (LOD) and the limit of quantification (LOQ) were between 0.001 and 0.002 microg g(-1) and between 0.004 and 0.006 microg g(-1), respectively. Recoveries were from 81.9% to 105.4% and precision less than 12.4%. The proposed method was successfully applied to routine analysis of the AVMs residues in rice.

Determination of two capsaicinoids in analgesic transdermal patches using RP-HPLC and UV spectroscopy

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F. Kobarfard

2017-11-01

Full Text Available Background and objectives: At the present time, a considerable frontier in the administration of therapeutic medications is transdermal drug delivery. Methods: In this study, a rapid, precise, sensitive and selective reversed-phasehigh performance liquid chromatography (RP-HPLC method has been evaluated, developed and validated to separate and quantitate capsaicin and dihydrocapsaicin (main active agents in analgesic dermal patches produced in Iran. Results: After isolation from laminated adhesive patches, capsaicinoids were analyzed on Lichrospher C18 analytical columns with reversed phase, using a mobile phase composition of methanol and distilled water (70:30 v/v and without any buffer (pH=6.5. The flow rate was 1 mL/min and the UV detector was operating at 281 nm. The assay was found to be linear over the range of 0.1-1.0 mg/mL. All validation parameters were within the acceptable range. Conclusion: It seems that the developed method was fairly sensitive and reliable in measuring capsaicinoids in commercially available analgesic transdermal patches in Iran.

A simple RP-HPLC method for related substances of zoledronic acid in pharmaceutical products

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L. Maheswara Reddy

2017-02-01

Full Text Available A novel, selective and sensitive reverse phase-high performance liquid chromatography (RP-HPLC method has been developed for the validated estimation of imidazol-1-yl-acetic acid in zoledronic acid formulations. The separation was achieved on a 5 μ C18 column (250 × 4.6 mm using a mobile phase that consists of the buffer (4.5 g of di-potassium hydrogen phosphate anhydrous and 2.0 g of tetra butyl ammonium hydrogen sulphate (TBAHS in 1000 mL of water and methanol in the ratio of 900:100 v/v. The flow rate was maintained at 1.0 mL min−1. The detection of the constituents was done at 215 nm using a UV detector. The retention times of imidazol-1-yl-acetic acid and zoledronic acid were 7.2 and 10.2 min respectively. Recovery studies were satisfactory and the correlation coefficient, 0.999 indicates linearity of the method within the limits. The developed method can be applicable for regular qualitative analysis.

Brazilian red propolis attenuates hypertension and renal damage in 5/6 renal ablation model.

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Flávio Teles

Full Text Available The pathogenic role of inflammation and oxidative stress in chronic kidney disease (CKD is well known. Anti-inflammatories and antioxidant drugs has demonstrated significant renoprotection in experimental nephropathies. Moreover, the inclusion of natural antioxidants derived from food and herbal extracts (such as polyphenols, curcumin and lycopene as an adjuvant therapy for slowing CKD progression has been largely tested. Brazilian propolis is a honeybee product, whose anti-inflammatory, antimicrobial and antioxidant effects have been widely shown in models of sepsis, cancer, skin irritation and liver fibrosis. Furthermore, previous studies demonstrated that this compound promotes vasodilation and reduces hypertension. However, potential renoprotective effects of propolis in CKD have never been investigated. The aim of this study was to evaluate the effects of a subtype of Brazilian propolis, the Red Propolis (RP, in the 5/6 renal ablation model (Nx. Adult male Wistar rats underwent Nx and were divided into untreated (Nx and RP-treated (Nx+RP groups, after 30 days of surgery; when rats already exhibited marked hypertension and proteinuria. Animals were observed for 90 days from the surgery day, when Nx+RP group showed significant reduction of hypertension, proteinuria, serum creatinine retention, glomerulosclerosis, renal macrophage infiltration and oxidative stress, compared to age-matched untreated Nx rats, which worsened progressively over time. In conclusion, RP treatment attenuated hypertension and structural renal damage in Nx model. Reduction of renal inflammation and oxidative stress could be a plausible mechanism to explain this renoprotection.

PTP1B deficiency improves hypothalamic insulin sensitivity resulting in the attenuation of AgRP mRNA expression under high-fat diet conditions.

Science.gov (United States)

Sugiyama, Mariko; Banno, Ryoichi; Mizoguchi, Akira; Tominaga, Takashi; Tsunekawa, Taku; Onoue, Takeshi; Hagiwara, Daisuke; Ito, Yoshihiro; Morishita, Yoshiaki; Iwama, Shintaro; Goto, Motomitsu; Suga, Hidetaka; Arima, Hiroshi

2017-06-17

Hypothalamic insulin receptor signaling regulates energy balance and glucose homeostasis via agouti-related protein (AgRP). While protein tyrosine phosphatase 1B (PTP1B) is classically known to be a negative regulator of peripheral insulin signaling by dephosphorylating both insulin receptor β (IRβ) and insulin receptor substrate, the role of PTP1B in hypothalamic insulin signaling remains to be fully elucidated. In the present study, we investigated the role of PTP1B in hypothalamic insulin signaling using PTP1B deficient (KO) mice in vivo and ex vivo. For the in vivo study, hypothalamic insulin resistance induced by a high-fat diet (HFD) improved in KO mice compared to wild-type (WT) mice. Hypothalamic AgRP mRNA expression levels were also significantly decreased in KO mice independent of body weight changes. In an ex vivo study using hypothalamic organotypic cultures, insulin treatment significantly increased the phosphorylation of both IRβ and Akt in the hypothalamus of KO mice compared to WT mice, and also significantly decreased AgRP mRNA expression levels in KO mice. While incubation with inhibitors of phosphatidylinositol-3 kinase (PI3K) had no effect on basal levels of Akt phosphorylation, these suppressed insulin induction of Akt phosphorylation to almost basal levels in WT and KO mice. The inhibition of the PI3K-Akt pathway blocked the downregulation of AgRP mRNA expression in KO mice treated with insulin. These data suggest that PTP1B acts on the hypothalamic insulin signaling via the PI3K-Akt pathway. Together, our results suggest a deficiency of PTP1B improves hypothalamic insulin sensitivity resulting in the attenuation of AgRP mRNA expression under HFD conditions. Copyright © 2017 Elsevier Inc. All rights reserved.

Apolipoprotein A-IV inhibits AgRP/NPY neurons and activates POMC neurons in the arcuate nucleus

Science.gov (United States)

Apolipoprotein A-IV (apoA-IV) in the brain potently suppresses food intake. However the mechanisms underlying its anorexigenic effects remain to be identified. We first examined the effects of apoA-IV on cellular activities in hypothalamic neurons that co-express agouti-related peptide (AgRP) and ne...

Simulación con el código MCNP del reactor nuclear RP-10 en su configuración #14, BOC

OpenAIRE

Lázaro, Gerardo; Parreño, Fernando

2001-01-01

Se presenta los resultados de exceso de reactividad del núcleo del reactor RP-10 en su configuración 14. Este exceso de reactividad ha sido calculado con MCNP4B con un modelo que describe en detalle las características de los elementos combustibles normales y de control, así como de cada elemento que constituye la configuración de trabajo #14. Este modelo fue previamente utilizado en el reactor RP-0 y ha sido aplicado en la configuración de arranque para el cálculo del exceso de reactividad y...

WE-AB-207B-02: A Bayesian Network Approach for Joint Prediction of Tumor Control and Radiation Pneumonitis (RP) in Non-Small-Cell Lung Cancer (NSCLC)

Energy Technology Data Exchange (ETDEWEB)

Luo, Y; McShan, D; Matuszak, M; Hobson, S; Jolly, S; Ten Haken, R; El Naqa, I [University of Michigan, Ann Arbor, MI (United States)

2016-06-15

Purpose: NSCLC radiotherapy treatment is a trade-off between controlling the tumor while limiting radiation-induced toxicities. Here we identify hierarchical biophysical relationships that could simultaneously influence both local control (LC) and RP by using an integrated Bayesian Networks (BN) approach. Methods: We studied 79 NSCLC patients treated on prospective protocol with 56 cases of LC and 21 events of RP. Beyond dosimetric information, each patient had 193 features including 12 clinical factors, 60 circulating blood cytokines before and during radiotherapy, 62 microRNAs, and 59 single-nucleotide polymorphisms (SNPs). The most relevant biophysical predictors for both LC and RP were identified using a Markov blanket local discovery algorithm and the corresponding BN was constructed using a score-learning algorithm. The area under the free-response receiver operating characteristics (AU-FROC) was used for performance evaluation. Cross-validation was employed to guard against overfitting pitfalls. Results: A BN revealing the biophysical interrelationships jointly in terms of LC and RP was developed and evaluated. The integrated BN included two SNPs, one microRNA, one clinical factor, three pre-treatment cytokines, relative changes of two cytokines between pre and during-treatment, and gEUDs of the GTV (a=-20) and lung (a=1). On cross-validation, the AUC prediction of independent LC was 0.85 (95% CI: 0.75–0.95) and RP was 0.83 (0.73–0.92). The AU-FROC of the integrated BN to predict both LC/RP was 0.81 (0.71–0.90) based on 2000 stratified bootstrap, indicating minimal loss in joint prediction power. Conclusions: We developed a new approach for multiple outcome utility application in radiotherapy based on integrated BN techniques. The BN developed from large-scale retrospective data is able to simultaneously predict LC and RP in NSCLC treatments based on individual patient characteristics. The joint prediction is only slightly compromised compared to

WE-AB-207B-02: A Bayesian Network Approach for Joint Prediction of Tumor Control and Radiation Pneumonitis (RP) in Non-Small-Cell Lung Cancer (NSCLC)

International Nuclear Information System (INIS)

Luo, Y; McShan, D; Matuszak, M; Hobson, S; Jolly, S; Ten Haken, R; El Naqa, I

2016-01-01

Purpose: NSCLC radiotherapy treatment is a trade-off between controlling the tumor while limiting radiation-induced toxicities. Here we identify hierarchical biophysical relationships that could simultaneously influence both local control (LC) and RP by using an integrated Bayesian Networks (BN) approach. Methods: We studied 79 NSCLC patients treated on prospective protocol with 56 cases of LC and 21 events of RP. Beyond dosimetric information, each patient had 193 features including 12 clinical factors, 60 circulating blood cytokines before and during radiotherapy, 62 microRNAs, and 59 single-nucleotide polymorphisms (SNPs). The most relevant biophysical predictors for both LC and RP were identified using a Markov blanket local discovery algorithm and the corresponding BN was constructed using a score-learning algorithm. The area under the free-response receiver operating characteristics (AU-FROC) was used for performance evaluation. Cross-validation was employed to guard against overfitting pitfalls. Results: A BN revealing the biophysical interrelationships jointly in terms of LC and RP was developed and evaluated. The integrated BN included two SNPs, one microRNA, one clinical factor, three pre-treatment cytokines, relative changes of two cytokines between pre and during-treatment, and gEUDs of the GTV (a=-20) and lung (a=1). On cross-validation, the AUC prediction of independent LC was 0.85 (95% CI: 0.75–0.95) and RP was 0.83 (0.73–0.92). The AU-FROC of the integrated BN to predict both LC/RP was 0.81 (0.71–0.90) based on 2000 stratified bootstrap, indicating minimal loss in joint prediction power. Conclusions: We developed a new approach for multiple outcome utility application in radiotherapy based on integrated BN techniques. The BN developed from large-scale retrospective data is able to simultaneously predict LC and RP in NSCLC treatments based on individual patient characteristics. The joint prediction is only slightly compromised compared to

All solutions of the Diophantine equation a^6+b^6=c^6+d^6+e^6+f^6+g^6 for a,b,c,d,e,f,g < 250000 found with a distributed Boinc project

OpenAIRE

Gerbicz, Robert; Meyrignac, Jean-Charles; Beckert, Uwe

2011-01-01

The above equation is also called as Euler(6,2,5) system. By computational aspect these systems are very interesting. And we can also apply these methods to other Diophantine equations. We give a brief history of these systems and how we searched for these big solutions on Boinc. Our two Boinc projects ran from April of 2010 to July of 2011.

Phase retrieval with the reverse projection method in the presence of object's scattering

International Nuclear Information System (INIS)

Wang, Zhili; Gao, Kun; Wang, Dajiang

2017-01-01

X-ray grating interferometry can provide substantially increased contrast over traditional attenuation-based techniques in biomedical applications, and therefore novel and complementary information. Recently, special attention has been paid to quantitative phase retrieval in X-ray grating interferometry, which is mandatory to perform phase tomography, to achieve material identification, etc. An innovative approach, dubbed “Reverse Projection” (RP), has been developed for quantitative phase retrieval. The RP method abandons grating scanning completely, and is thus advantageous in terms of higher efficiency and reduced radiation damage. Therefore, it is expected that this novel method would find its potential in preclinical and clinical implementations. Strictly speaking, the reverse projection method is applicable for objects exhibiting only absorption and refraction. In this contribution, we discuss the phase retrieval with the reverse projection method for general objects with absorption, refraction and scattering simultaneously. Especially, we investigate the influence of the object's scattering on the retrieved refraction signal. Both theoretical analysis and numerical experiments are performed. The results show that the retrieved refraction signal is the product of object's refraction and scattering signals for small values. In the case of a strong scattering, the reverse projection method cannot provide reliable phase retrieval. Those presented results will guide the use of the reverse projection method for future practical applications, and help to explain some possible artifacts in the retrieved images and/or reconstructed slices. - Highlights: • Accurate phase retrieval by the reverse projection method without object's scattering. • Retrieved refraction signal contaminated by the object's scattering. • Refraction signal underestimated by the reverse projection method. • Guide the use of the reverse projection method for

Radiation Protection Knowledge, Attitude and Practice (RP-KAP) as Predictors of Job Stress Among Radiation Workers in Tehran Province, Iran.

Science.gov (United States)

Alavi, S Shohreh; Taghizadeh Dabbagh, Sima; Abbasi, Mahya; Mehrdad, Ramin

2016-10-01

In recent years, much attention has been paid to occupational stress, but relatively little or no research has been conducted on the influence of knowledge, attitude, and practice of radiation protection (RP-KAP) on job stress among radiation workers. This study aims to assess job stress among health care workers in Iran who are occupationally exposed to radiation in order to determine the effects of KAP on self-protection against radiation on their job stress. The population in this descriptive cross-sectional study comprised 670 healthcare workers, including 428 staff with a degree in radiology and 242 other medical personnel who were working in 16 hospitals affiliated with Tehran University of Medical Sciences (TUMS) in Tehran, Iran. The census method was used to sample the workers. In total, 264 staff with a degree in radiology and 149 other medical personnel completed the job content questionnaire (JCQ) and the RP-KAP questionnaire from May to November 2014. The prevalence rate of job stress was 22.5% based on calculation formulas and possible scores on the JCQ. Sex, RP-knowledge, attitude, practice, and in-service training predicted 41.8% of the variance in job stress. According to the results of the binary logistic regression, workers with higher scores on knowledge (OR = 0.82, 95% CI: 0.75 - 0.90), attitude (OR = 0.71, 95% CI: 0.63 - 0.82), and practice (OR = 0.78, 95% CI: 0.72 - 0.86) and those who had participated in training programs had significantly lower rates of job stress (OR = 0.51, 95% CI: 0.28 - 0.93). The effects of RP knowledge, attitude, and practice on job stress were significant. In order to reduce job stress in radiation environments, ongoing training programs related to self-care and protection principles are recommended.

Positive experience in the construction and project management of Kashiwazaki-Kariwa no. 6 and no. 7

International Nuclear Information System (INIS)

Minematsu, A.

1999-01-01

Kashiwazaki-Kariwa No.6 and No.7 (K-6/7), the world first Advanced Boiling Water Reactor (ABWR) units, started commercial operations on November 7, 1996 and July 2, 1997 respectively. ABWR has been developed as a standard design of the next generation BWR to meet common goals set by the Japanese electric utilities and BWR manufacturers (GE, Hitachi and Toshiba) based on our design, construction, operation and maintenance experience of nuclear power plants. The construction of K-6/7 and confirmatory tests for the verification of the first-of-a-kind (FOAK) design features of ABWR were conducted smoothly without any delay. The duration of the construction was 51.5 months. It was shorter than conventional BWRs in Japan by nearly one year. This was realized by design features of ABWR for better constructability, a principle of 'test before use' applied to the FOAKs, advanced construction technology, detailed engineering at very early stages of the project, and good construction management. The positive experience in the K-6/7 project is now being reviewed and standardized for next ABWR projects. The data and knowledge accumulated through the K-6/7 project will be utilized effectively with the aide of the latest information technology. (author)

EASYTRAC Project: Work package 6.4 Reversal technique to calibrate gear and thread standards

DEFF Research Database (Denmark)

Carmignato, Simone; De Chiffre, Leonardo; Larsen, Erik

This report was produced as a part of the project EASYTRAC, an EU project under the programme Competitive and Sustainable Growth: Contract No. G6RD-CT-2000-00188, coordinated by UNIMETRIK S.A. (Spain). The project is concerned with low uncertainty calibrations on coordinate measuring machines (CM...... (PTB) - Germany and Tampere University of Technology (TUT) - Finland. The present report describes feasibility and experimental results of a reversal and substitute element technique application for thread calibration on CMMs....

Improved method for reliable HMW-GS identification by RP-HPLC and SDS-PAGE in common wheat cultivars

Science.gov (United States)

The accurate identification of alleles for high-molecular weight glutenins (HMW-GS) is critical for wheat breeding programs targeting end-use quality. RP-HPLC methods were optimized for separation of HMW-GS, resulting in enhanced resolution of 1By and 1Dx subunits. Statistically significant differe...

Monitoring and calibration of the ALICE time projection chamber

CERN Document Server

Larsen, Dag Toppe

The aim of the A Large Ion Collider Experiment (ALICE) experiment at CERN is to study the properties of the Quark–Gluon Plasma (QGP). With energies up to 5.5 A T eV for Pb+Pb collisions, the Large Hadron Collider (LHC) sets a new benchmark for heavy- ion collisions, and opens the door to a so far unexplored energy domain. A closer look at some of the physics topics of ALICE is given in Chapter 1. ALICE consists of several sub-detectors and other sub-systems. The various sub- detectors are designed for exploring different aspects of the particle production of an heavy-ion collision. Chapter 2 gives some insight into the design. The main tracking detector is the Time Projection Chamber (TPC). It has more than half million read-out channels, divided into 216 Read-out Partitions (RPs). Each RP is a separate Front-End Electronics (FEE) entity, as described in Chapter 3. A complex Detector Control System (DCS) is needed for configuration, monitoring and control. The heart of it on the RP side is a small embedded ...

Application of modern information technologies for monitoring of 600 RP key component performance and lifetime assessment

International Nuclear Information System (INIS)

Karpenko, S.N.; Korotkih, Yu.G.; Levin, A.A.; Sankov, E.I.; Pobedonostzev, A.B.; Shashkin, S.L.

1997-01-01

Safety and reliability of NPP operation in the first turn depend on reliability and quality of the main technological equipment. Diagnostics systems are envisaged for the equipment state inspection to determine its serviceability in NPP designs. An assessment of the equipment residual lifetime at operating plants is calculated as a difference between specified (according to the operation model accepted in the design phase) lifetime and actual time in operation. Real influence of operating modes on the main equipment lifetime, in this case, is not taken into account. Within a design of NPP with VPBER-600 passive safety RP a system for lifetime assessment of RP key component is provided as a part of a process control system. Specialists from OKBM and Scientific Research Institute of Mechanics participate in development of this system. Now the first stage of the work on creation of this system is completed. This paper describes the purpose, the basic configuration principles of the system, its position among other NPP systems, organization of user-system interaction. (author). 5 refs, 1 figs

Application of modern information technologies for monitoring of 600 RP key component performance and lifetime assessment

Energy Technology Data Exchange (ETDEWEB)

Karpenko, S N; Korotkih, Yu G; Levin, A A; Sankov, E I [Research Inst. of Mechanics of Lobachevski State Univ., Nizhny Novgorod (Russian Federation); Pobedonostzev, A B; Shashkin, S L [OKB Mechanical Engineering, Nizhny Novgorod (Russian Federation)

1997-07-01

Safety and reliability of NPP operation in the first turn depend on reliability and quality of the main technological equipment. Diagnostics systems are envisaged for the equipment state inspection to determine its serviceability in NPP designs. An assessment of the equipment residual lifetime at operating plants is calculated as a difference between specified (according to the operation model accepted in the design phase) lifetime and actual time in operation. Real influence of operating modes on the main equipment lifetime, in this case, is not taken into account. Within a design of NPP with VPBER-600 passive safety RP a system for lifetime assessment of RP key component is provided as a part of a process control system. Specialists from OKBM and Scientific Research Institute of Mechanics participate in development of this system. Now the first stage of the work on creation of this system is completed. This paper describes the purpose, the basic configuration principles of the system, its position among other NPP systems, organization of user-system interaction. (author). 5 refs, 1 figs.

Evolutionary algorithm-based multi-criteria optimization of triboelectrostatic separator

Czech Academy of Sciences Publication Activity Database

Mach, F.; Adam, L.; Kacerovský, J.; Karban, P.; Doležel, Ivo

2014-01-01

Roč. 270, November (2014), s. 134-142 ISSN 0377-0427 R&D Projects: GA ČR(CZ) GAP102/11/0498 Institutional support: RVO:61388998 Keywords : separation processes * triboelectricity * particle separators Subject RIV: JA - Electronics ; Optoelectronics, Electrical Engineering Impact factor: 1.266, year: 2014

hp-FEM electromechanical transduction model of ionic polymer metal composites

Czech Academy of Sciences Publication Activity Database

Pugal, D.; Šolín, Pavel; Kim, K.; Aabloo, A.

2014-01-01

Roč. 260, April (2014), s. 135-148 ISSN 0377-0427 R&D Projects: GA ČR(CZ) GAP102/11/0498 Institutional support: RVO:61388998 Keywords : hp-FEM * Nernst-Planck * Poisson Subject RIV: JA - Electronics ; Optoelectronics, Electrical Engineering Impact factor: 1.266, year: 2014

RP-HPLC-DAD-ESI-QTOF-MS based metabolic profiling of the potential Olea europaea by-product "wood" and its comparison with leaf counterpart.

Science.gov (United States)

Ammar, Sonda; Contreras, Maria Del Mar; Gargouri, Boutheina; Segura-Carretero, Antonio; Bouaziz, Mohamed

2017-05-01

Olea europaea L. organs such as leaves, stems and roots have been associated with numerous in vivo and in vitro biological activities and used for traditional medicinal purposes. However, tree wood is an untapped resource with little information about their chemical composition. That is why, the objective of this study is to increase the knowledge about phytochemicals from 'Chemlali' olive wood by means of mass spectrometry-based analyses. Its comparison with by-products derived from leaves was also studied. Hydromethanol extracts from wood and leaves with stems of 'Chemlali' olive cultivar were analysed using reversed-phase (RP) high-performance liquid chromatography (HPLC) coupled to two detection systems: diode-array detection (DAD) and quadrupole time-of-flight (QTOF) mass spectrometry (MS) in negative ion mode. Tandem MS experiments were performed to establish the chemical structure of olive phytochemicals. A total of 85 compounds were characterised in the studied olive parts and classified as: sugars (3), organic acids (5), one phenolic aldehyde, simple phenolic acids (6), simple phenylethanoids (5), flavonoids (14), coumarins (3), caffeoyl phenylethanoid derivatives (6), iridoids (5), secoiridoids (32), and lignans (5). To our knowledge, the major part of these metabolites was not previously reported in olive tree wood, and 10 olive chemical constituents were identified for the first time in the Oleaceae family. The results presented here demonstrated the usefulness of the methodology proposed, based on RP-HPLC-DAD-ESI-QTOF-MS and MS/MS, to develop an exhaustive metabolic profiling and to recover new biologically active compounds in olive wood with pharmacologic and cosmetic potential. Copyright © 2017 John Wiley & Sons, Ltd. Copyright © 2017 John Wiley & Sons, Ltd.

Integrated project 'fundamental processes of radionuclide migration (FUNMIG)', within EC 6. framework programme

International Nuclear Information System (INIS)

Buckau, G.

2005-01-01

Full text of publication follows: The FUNMIG project is an integrated project within the 6. Framework Programme of the European Commissions. It started 1 January 2005 and has a duration of 4 years. The project deals with all aspects of radionuclide migration in the far-field of a high level nuclear waste repository. It is structured into seven components. Two components are on fundamental processes applicable to all nuclear waste disposal concepts and host-rock types. They are divided into processes that are conceptually well understood and one component dealing with processes where not only trustworthy data are scarce, but conceptualization is at an infant state. Three components address processes specific for host rock types presently under discussion for high level waste disposal in the EU, namely clay rich, crystalline and salt rock. One component is on integration of processes and abstraction to performance assessment. Finally, one component deals with a broad spectrum of activities on knowledge transfer and training. There are 51 contractors from 15 European countries involved. In addition, a special instrument is used for groups interested in participation as Associated Groups. There are presently about 15 such Associated Groups increasing the number of countries involved to 17. Among these Associated Groups, presently 8 national regulatory bodies are represented, promoting communication between the scientific-technical and regulatory communities. There is no specific deadline for joining the FUNMIG project as an Associated Group and negotiations with further groups/organizations is ongoing. Information about the FUNMIG project can be found under www.funmig.com. The present project is one out of four dealing with disposal of radioactive waste within the European Commissions 6. Framework Programme. The other ones are on the basis for waste disposal techniques, near field processes, and a project expected within the next future dealing with performance

Comparison of radiation doses between newborns and 6-y-old children undergoing head, chest and abdominal CT examinations-A phantom study

International Nuclear Information System (INIS)

Sugimoto, N.; Aoyama, T.; Koyama, S.; Yamauchi-Kawaura, C.; Fujii, K.

2013-01-01

Radiation doses in paediatric computed tomography (CT) were investigated for various types of recent CT scanners with newborn and 6-y-old phantoms in which silicon-photodiode dosemeters were implanted at various organ positions. In the head, chest and abdominal CT for the newborn phantom, doses for organs within the scan region were 21-40, 3-8 and 3-12 mGy, respectively. The corresponding doses for the child phantom were 20-37, 2-11 and 4-17 mGy, respectively. In the head, chest and abdominal CT, the effective doses were respectively 2.1-3.3, 2.0-6.0 and 2.2-10.0 mSv for the newborn, and 1.0-2.0, 1.2-6.6 and 2.9-11.8 mSv for the child. Radiation doses for the newborn were at the same levels as those for the child, excepting effective doses in head CT for the newborn, which were 1.8 times higher than those for the child. (authors)

Analysis Study of Stevioside and Rebaudioside A from Stevia rebaudiana Bertoni by Normal Phase SPE and RP-HPLC

Science.gov (United States)

Martono, Y.; Rohman, A.; Riyanto, S.; Martono, S.

2018-04-01

Solid Phase Extraction (SPE) method using silica as sorbent for stevioside and rebaudiosida A analysis in Stevia rebaudiana Bertoni leaf have not been performed. The aim of this study is to develop SPE method using silica as sorbent for Reverse Phase-High Performance Liquid Chromatography (RP-HPLC) analysis of stevioside and rebaudiosida A in S. rebaudiana leaf. The results of this study indicate that the optimal conditions for normal phase SPE (silica) are conditioned with 3.0 mL of hexane. The sample loading volume is 0.1 mL. Cartridge is eluted with 1.0 mL acetonitrile: water (80: 20, v/v) to separate both analytes. The cartridge is washed with chloroform and water of 0.3 mL respectively. The developed SPE sample preparation method meets the accuracy and precision test and can be used for the analysis of stevioside and rebaudioside A by RP-HPLC.

University of Chicago School Mathematics Project 6-12 Curriculum. What Works Clearinghouse Intervention Report

Science.gov (United States)

What Works Clearinghouse, 2011

2011-01-01

The "University of Chicago School Mathematics Project ("UCSMP") 6-12 Curriculum" is a series of yearlong courses--(1) Transition Mathematics; (2) Algebra; (3) Geometry; (4) Advanced Algebra; (5) Functions, Statistics, and Trigonometry; and (6) Precalculus and Discrete Mathematics--emphasizing problem solving, real-world applications, and the use…

Activación del topacio natural irradiado por neutrones en el núcleo del reactor RP-10

OpenAIRE

Gómez, J.; Parreño, Fernando; Lázaro, Gerardo; Vela, Mariano

2003-01-01

Se obtuvieron cristales de topacio activados al ser irradiados con neutrones dentro del núcleo del reactor RP-10. La activación depende del flujo de neutrones, por ello se desarrolló portamuestras (canes de irradiación) para absorber que son los causantes de la activación

Image reconstruction from projections and its application in emission computer tomography

International Nuclear Information System (INIS)

Kuba, Attila; Csernay, Laszlo

1989-01-01

Computer tomography is an imaging technique for producing cross sectional images by reconstruction from projections. Its two main branches are called transmission and emission computer tomography, TCT and ECT, resp. After an overview of the theory and practice of TCT and ECT, the first Hungarian ECT type MB 9300 SPECT consisting of a gamma camera and Ketronic Medax N computer is described, and its applications to radiological patient observations are discussed briefly. (R.P.) 28 refs.; 4 figs

UBERA-6 project: Achievements of 4 working years

International Nuclear Information System (INIS)

Blaumann, H; Fernandez, C; Dell Occhio, L; D Ovidio, C; Fabro, J; Miceli, M; Novara O; Perez, A; Taboada, H

2009-01-01

On May 2005 the President of CNEA created the UBERA-6 project, belonging to the former Technology and Environmental Management, with the aim to convert to Low Enriched Uranium (LEU) the RA-6 reactor core, to swap with the US Department of Energy (US-DoE) equivalent inventories of High Enriched Uranium (HEU) for LEU, to export to USA the spent HEU core and to recover and downblend to LEU remnant HEU inventories contained in fuel and irradiation target scraps. By means of two contracts signed by CNEA and US-DoE, acquisition of consumables and graphite reflectors, the fabrication of LEU core replacement, conditioning, transport and exportation of spent HEU core and subsequent supply of fresh LEU for fuel and irradiation targets used in our research reactors were costed. During July, 2006 468 HEU based fresh plates were exported to USA. On June 30th, 2007 the RA-6 reactor temporarily stopped working and its personnel remover the HEU core to the auxiliary pool. On November 7th the former spent HEU based core was exported to USA. During May and July, 2008 the new RA-6 reactor LEU based core and control assemblies were provided. During March, 2009 the RA-6 reactor became critical. For recovering and blending down of remnant HEU inventories, the Triple Height Laboratory (LTA) was refurbished. A Supplemental Agreement to one of the original contract between CNEA and US-DoE will financially support the refurbishment of the Radiochemical Facility Laboratory (LFR) and so reprocess irradiated HEU retained in radioisotope production filters to downblend into LEU, as well as the separation of the pair Sr90-Y90 and of Cs137 inventories for further application in Nuclear Medicine. [es

First description of the male of Philometra filiformis (Nematoda: Philometridae), a gonad-infecting parasite of the marine fish Pagellus erythrinus (Sparidae) in Mediterranean

Czech Academy of Sciences Publication Activity Database

Gaglio, G.; Gianetto, S.; Panebianco, A.; Moravec, František

2009-01-01

Roč. 56, č. 4 (2009), s. 317-318 ISSN 0015-5683 R&D Projects: GA MŠk LC522 Institutional research plan: CEZ:AV0Z60220518 Keywords : Philometra * Pagellus * Italy Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 1.266, year: 2009

Female morphology of Philometra rubra (Nematoda: Philometridae), a parasite of the abdominal cavity of the striped sea-bass Morone saxatilis (Moronidae, Perciformes) in the USA

Czech Academy of Sciences Publication Activity Database

Moravec, František; Dyková, Iva; de Buron, I.

2009-01-01

Roč. 56, č. 1 (2009), s. 64-66 ISSN 0015-5683 R&D Projects: GA MŠk LC522 Institutional research plan: CEZ:AV0Z60220518 Keywords : Philometra * Morone * USA Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 1.266, year: 2009

RP-UHPLC-UV-ESI-MS/MS analysis of LPMO generated C4-oxidized gluco-oligosaccharides after non-reductive labeling with 2-aminobenzamide.

Science.gov (United States)

Frommhagen, Matthias; van Erven, Gijs; Sanders, Mark; van Berkel, Willem J H; Kabel, Mirjam A; Gruppen, Harry

2017-08-07

Lytic polysaccharide monooxygenases (LPMOs) are able to cleave recalcitrant polysaccharides, such as cellulose, by oxidizing the C1 and/or C4 atoms. The analysis of the resulting products requires a variety of analytical techniques. Up to now, these techniques mainly focused on the identification of non-oxidized and C1-oxidized oligosaccharides. The analysis of C4-oxidized gluco-oligosaccharides is mostly performed by using high pressure anion exchange chromatography (HPAEC). However, the alkaline conditions used during HPAEC analysis lead to tautomerization of C4-oxidized gluco-oligosaccharides, which limits the use of this technique. Here, we describe the use of reverse phase-ultra high performance liquid chromatography (RP-UHPLC) in combination with non-reductive 2-aminobenzamide (2-AB) labeling. Non-reductive 2-AB labeling enabled separation of C4-oxidized gluco-oligosaccharides from their non-oxidized counterparts. Moreover, RP-UHPLC does not require buffered mobile phases, which reduce mass spectrometry (MS) sensitivity. The latter is seen as an advantage over other techniques such as hydrophilic interaction liquid chromatography and porous graphitized carbon coupled to MS. RP-UHPLC coupled to UV detection and mass spectrometry allowed the identification of both labeled non-oxidized and C4-oxidized oligosaccharides. Non-reductive labeling kept the ketone at the C4-position of LPMO oxidized oligosaccharides intact, while selective reducing agents such as sodium triacetoxyborohydride (STAB) reduced this ketone group. Our results show that RP-UHPLC-UV-ESI-MS in combination with non-reductively 2-AB labeling is a suitable technique for the separation and identification of LPMO-generated C4-oxidized gluco-oligosaccharides. Copyright © 2017 The Authors. Published by Elsevier Ltd.. All rights reserved.

Department of Energy review of the National Spallation Neutron Source Project

Energy Technology Data Exchange (ETDEWEB)

NONE

1997-06-01

A Department of Energy (DOE) review of the Conceptual Design Report (CDR) for the National Spallation Neutron Source (NSNS) was conducted. The NSNS will be a new high-power spallation neutron source; initially, it will operate at 1 megawatt (MW), but is designed to be upgradeable to significantly higher power, at lower cost, when accelerator and target technologies are developed for higher power. The 53-member Review Committee examined the projected cost, schedule, technical scope, and management structure described in the CDR. For each of the major components of the NSNS, the Committee determined that the project team had produced credible designs that can be expected to work well. What remains to be done is to integrate the design of these components. With the exception of the liquid mercury target, the NSNS Project will rely heavily on proven technologies and, thus, will face a relatively low risk to successful project completion. The Total Project Cost (TPC) presented to the Committee in the CDR was $1.266 billion in as-spent dollars. In general, the Committee felt that the laboratory consortium had presented a credible estimate for each of the major components but that value engineering might produce some savings. The construction schedule presented to the Committee covered six years beginning in FY 1999. The Committee questioned whether all parts of the project could be completed according to this schedule. In particular, the linac and the conventional facilities appeared to have overly optimistic schedules. The NSNS project team was encouraged to reexamine these activities and to consider a more conservative seven-year schedule. Another concern of the Committee was the management structure. In summary, the Committee felt that this Conceptual Design Report was a very credible proposal, and that there is a high probability for successful completion of this major project within the proposed budget, although the six-year proposed schedule may be optimistic.

Department of Energy review of the National Spallation Neutron Source Project

International Nuclear Information System (INIS)

1997-06-01

A Department of Energy (DOE) review of the Conceptual Design Report (CDR) for the National Spallation Neutron Source (NSNS) was conducted. The NSNS will be a new high-power spallation neutron source; initially, it will operate at 1 megawatt (MW), but is designed to be upgradeable to significantly higher power, at lower cost, when accelerator and target technologies are developed for higher power. The 53-member Review Committee examined the projected cost, schedule, technical scope, and management structure described in the CDR. For each of the major components of the NSNS, the Committee determined that the project team had produced credible designs that can be expected to work well. What remains to be done is to integrate the design of these components. With the exception of the liquid mercury target, the NSNS Project will rely heavily on proven technologies and, thus, will face a relatively low risk to successful project completion. The Total Project Cost (TPC) presented to the Committee in the CDR was $1.266 billion in as-spent dollars. In general, the Committee felt that the laboratory consortium had presented a credible estimate for each of the major components but that value engineering might produce some savings. The construction schedule presented to the Committee covered six years beginning in FY 1999. The Committee questioned whether all parts of the project could be completed according to this schedule. In particular, the linac and the conventional facilities appeared to have overly optimistic schedules. The NSNS project team was encouraged to reexamine these activities and to consider a more conservative seven-year schedule. Another concern of the Committee was the management structure. In summary, the Committee felt that this Conceptual Design Report was a very credible proposal, and that there is a high probability for successful completion of this major project within the proposed budget, although the six-year proposed schedule may be optimistic

Oncolytic Herpes Virus rRp450 Shows Efficacy in Orthotopic Xenograft Group 3/4 Medulloblastomas and Atypical Teratoid/Rhabdoid Tumors

Directory of Open Access Journals (Sweden)

Adam W. Studebaker

2017-09-01

Full Text Available Pediatric brain tumors including medulloblastoma and atypical teratoid/rhabdoid tumor are associated with significant mortality and treatment-associated morbidity. While medulloblastoma tumors within molecular subgroups 3 and 4 have a propensity to metastasize, atypical teratoid/rhabdoid tumors frequently afflict a very young patient population. Adjuvant treatment options for children suffering with these tumors are not only sub-optimal but also associated with many neurocognitive obstacles. A potentially novel treatment approach is oncolytic virotherapy, a developing therapeutic platform currently in early-phase clinical trials for pediatric brain tumors and recently US Food and Drug Administration (FDA-approved to treat melanoma in adults. We evaluated the therapeutic potential of the clinically available oncolytic herpes simplex vector rRp450 in cell lines derived from medulloblastoma and atypical teratoid/rhabdoid tumor. Cells of both tumor types were supportive of virus replication and virus-mediated cytotoxicity. Orthotopic xenograft models of medulloblastoma and atypical teratoid/rhabdoid tumors displayed significantly prolonged survival following a single, stereotactic intratumoral injection of rRp450. Furthermore, addition of the chemotherapeutic prodrug cyclophosphamide (CPA enhanced rRp450’s in vivo efficacy. In conclusion, oncolytic herpes viruses with the ability to bioactivate the prodrug CPA within the tumor microenvironment warrant further investigation as a potential therapy for pediatric brain tumors.

Mice deficient in ribosomal protein S6 phosphorylation suffer from muscle weakness that reflects a growth defect and energy deficit.

Directory of Open Access Journals (Sweden)

Igor Ruvinsky

Full Text Available BACKGROUND: Mice, whose ribosomal protein S6 cannot be phosphorylated due to replacement of all five phosphorylatable serine residues by alanines (rpS6(P-/-, are viable and fertile. However, phenotypic characterization of these mice and embryo fibroblasts derived from them, has established the role of these modifications in the regulation of the size of several cell types, as well as pancreatic beta-cell function and glucose homeostasis. A relatively passive behavior of these mice has raised the possibility that they suffer from muscle weakness, which has, indeed, been confirmed by a variety of physical performance tests. METHODOLOGY/PRINCIPAL FINDINGS: A large variety of experimental methodologies, including morphometric measurements of histological preparations, high throughput proteomic analysis, positron emission tomography (PET and numerous biochemical assays, were used in an attempt to establish the mechanism underlying the relative weakness of rpS6(P-/- muscles. Collectively, these experiments have demonstrated that the physical inferiority appears to result from two defects: a a decrease in total muscle mass that reflects impaired growth, rather than aberrant differentiation of myofibers, as well as a diminished abundance of contractile proteins; and b a reduced content of ATP and phosphocreatine, two readily available energy sources. The abundance of three mitochondrial proteins has been shown to diminish in the knockin mouse. However, the apparent energy deficiency in this genotype does not result from a lower mitochondrial mass or compromised activity of enzymes of the oxidative phosphorylation, nor does it reflect a decline in insulin-dependent glucose uptake, or diminution in storage of glycogen or triacylglycerol (TG in the muscle. CONCLUSIONS/SIGNIFICANCE: This study establishes rpS6 phosphorylation as a determinant of muscle strength through its role in regulation of myofiber growth and energy content. Interestingly, a similar

Evaluation and standardization of neutron activation analysis according to the K0 method in the RP-10 reactor

International Nuclear Information System (INIS)

Montoya R, E.

1995-01-01

It has been characterized and standardized an irradiation of the RP-10 Research Nuclear Reactor for use of the K 0 method of neutron activation analysis using the Hoegdahl convention; also it has been evaluate the behaviour of such method in regard to the accuracy and precision of the results obtained in the quantitative multi elemental analysis of several certified materials of reference. In order to prove that the analytical method is totally under statistical control, it has been used the Heydorn method. It has been verified that the method is exact, precise and reliable to determine the aluminium, antimuonium, arsenic, bromine, calcium, chloride, copper, magnesium, manganese, sodium, titanium, vanadium, zinc and other elements. Also, they are discussed, in regard to the use of K 0 constants, the different formalisms employed to calculate the integral of the reaction rate by nucleus in the activation. (author). 58 refs., 18 tabs., 6 figs

Experimental investigation on flow patterns of RP-3 kerosene under sub-critical and supercritical pressures

Science.gov (United States)

Wang, Ning; Zhou, Jin; Pan, Yu; Wang, Hui

2014-02-01

Active cooling with endothermic hydrocarbon fuel is proved to be one of the most promising approaches to solve the thermal problem for hypersonic aircraft such as scramjet. The flow patterns of two-phase flow inside the cooling channels have a great influence on the heat transfer characteristics. In this study, phase transition processes of RP-3 kerosene flowing inside a square quartz-glass tube were experimentally investigated. Three distinct phase transition phenomena (liquid-gas two phase flow under sub-critical pressures, critical opalescence under critical pressure, and corrugation under supercritical pressures) were identified. The conventional flow patterns of liquid-gas two phase flow, namely bubble flow, slug flow, churn flow and annular flow are observed under sub-critical pressures. Dense bubble flow and dispersed flow are recognized when pressure is increased towards the critical pressure whilst slug flow, churn flow and annular flow disappear. Under critical pressure, the opalescence phenomenon is observed. Under supercritical pressures, no conventional phase transition characteristics, such as bubbles are observed. But some kind of corrugation appears when RP-3 transfers from liquid to supercritical. The refraction index variation caused by sharp density gradient near the critical temperature is thought to be responsible for this corrugation.

Generic detection of poleroviruses using an RT-PCR assay targeting the RdRp coding sequence.

Science.gov (United States)

Lotos, Leonidas; Efthimiou, Konstantinos; Maliogka, Varvara I; Katis, Nikolaos I

2014-03-01

In this study a two-step RT-PCR assay was developed for the generic detection of poleroviruses. The RdRp coding region was selected as the primers' target, since it differs significantly from that of other members in the family Luteoviridae and its sequence can be more informative than other regions in the viral genome. Species specific RT-PCR assays targeting the same region were also developed for the detection of the six most widespread poleroviral species (Beet mild yellowing virus, Beet western yellows virus, Cucurbit aphid-borne virus, Carrot red leaf virus, Potato leafroll virus and Turnip yellows virus) in Greece and the collection of isolates. These isolates along with other characterized ones were used for the evaluation of the generic PCR's detection range. The developed assay efficiently amplified a 593bp RdRp fragment from 46 isolates of 10 different Polerovirus species. Phylogenetic analysis using the generic PCR's amplicon sequence showed that although it cannot accurately infer evolutionary relationships within the genus it can differentiate poleroviruses at the species level. Overall, the described generic assay could be applied for the reliable detection of Polerovirus infections and, in combination with the specific PCRs, for the identification of new and uncharacterized species in the genus. Copyright © 2013 Elsevier B.V. All rights reserved.

Transportation research synthesis : state DOT experiences with Primavera P6 project management software.

Science.gov (United States)

2010-03-01

The eight agencies we interviewed all reported general satisfaction with Primavera P6 as a project management tool within their organizations, although they noted that a significant commitment to training is required. Most states have not implemented...

Calculating the price of tanks, vessels and process equipment of petrochemical industry second criteria of integrity and survival remaining of API RP 579 (Fitness for service); Calculo do preco de tanques, vasos e equipamentos de processo da industria petroquimica segundo criterios de integridade e sobrevida remanescente do API RP 579 (Fitness for service)

Energy Technology Data Exchange (ETDEWEB)

Morato, Paulo Cesar Vidal Morato [Petroleo Brasileiro S.A. (PETROBRAS), Rio de Janeiro, RJ (Brazil)

2012-07-01

By owning many tanks, vessels and process equipment, PETROBRAS has developed the concept of 'Fitness-For-Service' (suitability for use) under the standard API RP 579, i.e. to verify the structural integrity and remaining useful life of equipment in service. In this paper we will discuss how to calculate the remaining useful life of equipment used in accordance with such criteria and with this technical data, calculate the depreciated price. Steps: verification of applicability; surveys of the technical data of the equipment; surveys the minimum thickness of plating equipment over the years; calculation of the average annual rate of corrosion (tc); calculation of the required minimum thickness according to the criteria of API RP 579 (tr); calculation of remaining useful life (nr); calculation of the depreciated price (Vd) equipment. Conclusions: intended for evaluation of tanks price, vessels and process equipment according to API RP 579 concepts. Estimate the remaining useful life of equipment used and calculates the depreciated price. Scientific method based, consistent and robust, due to calculating established the remaining useful life. (author)

The problem of early continence recovery after radical prostatectomy

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A. A. Kachmazov

2017-01-01

Full Text Available Background. The highest rate of incontinence after radical prostatectomy (RP is observed in the first 2–6 months after surgery. In order  to decrease the period of incontinence, virous surgical methods aimed at preservation and reconstruction of structures participating  in the mechanism of urine retention has been developed and improved.The study objective is to evaluate effectiveness of an original method of formation of an urethral anastomosis in the early continence recovery.Materials and methods. Data on 126 patients who underwent retropubic RP were analyzed. Depending on the method of urethral anastomosis formation, patients were divided into 2 groups: in the 1st group (n = 52 the patients underwent PR with urethra suspension m. levator ani, in the 2nd (n = 74 – standard RP. In the 1st group, 6 ligatures were applied to the anterior and posterior urethra walls: at the 12 and 6 hour projections through the mucosa, submucosa, and smooth muscle; at the 10, 2, 4, and 8 hour projections – more laterally with capture of the m. levator ani medial margins. Continence recovery was evaluated on days 1, 7, and 14 after urinary catheter removal and then at days 30, 90, 180, and 365 after RP. The criteria of continence were absence of urine leakage at rest and during physical activity and a necessity of using a safety liner.Results. There weren’t any significant differences at day 1 after urinary catheter removal between the two groups (р > 0.05. In the 1st group, continence values at days 30, 90, and 180 after RP were significantly higher (57.7, 69.2, and 71.1 %, respectively compared to the 2nd group (35.1, 41.9, and 51.3 %, respectively (р <0.05.Conclusion. Results of this work show significant benefits of RP with urethra suspension m. levator ani compared to standard RP per continence recovery criteria at days 7, 14, 30, 90, and 180 after the surgery. The technique of urethra suspension m. levator ani is easy to perform and ensures

EU-project AEROJET. Non-intrusive measurements of aircraft engine exhaust emissions

Energy Technology Data Exchange (ETDEWEB)

Schaefer, K.; Heland, J. [Fraunhofer-Inst. fuer Atmosphaerische Umweltforschung (IFU), Garmisch-Partenkirchen (Germany); Burrows, R. [Rolls-Royce Ltd. (United Kingdom). Engine Support Lab.; Bernard, M. [AUXITROL, S.A. (France). Aerospace Equipment Div.; Bishop, G. [British Aerospace (United Kingdom). Sowerby Research Centre; Lindermeir, E. [Deutsche Forschungsanstalt fuer Luft- und Raumfahrt e. V. (DLR), Bonn (Germany). Inst. fuer Optoelektronik; Lister, D.H. [Defence and Research Agency, Hants (United Kingdom). Propulsion and Development Dept.; Wiesen, P. [Bergische Univ. Wuppertal (Gesamthochshule) (Germany); Hilton, M. [University of Reading (United Kingdom). Dept. of Physics

1997-12-31

The main goal of the AEROJET programme is to demonstrate the equivalence of remote measurement techniques to conventional extractive methods for both gaseous and particulate measurements. The different remote measurement techniques are compared and calibrated. A demonstrator measurement system for exhaust gases, temperature and particulates including data-analysis software is regarded as result of this project. Non-intrusive measurements are the method of choice within the AEROJET project promising to avoid the disadvantages of the gas sampling techniques which are currently used. Different ground based non-intrusive measurement methods are demonstrated during a final evaluation phase. Several non-intrusive techniques are compared with conventional gas sampling and analysis techniques. (R.P.) 3 refs.

EU-project AEROJET. Non-intrusive measurements of aircraft engine exhaust emissions

Energy Technology Data Exchange (ETDEWEB)

Schaefer, K; Heland, J [Fraunhofer-Inst. fuer Atmosphaerische Umweltforschung (IFU), Garmisch-Partenkirchen (Germany); Burrows, R [Rolls-Royce Ltd. (United Kingdom). Engine Support Lab.; Bernard, M [AUXITROL, S.A. (France). Aerospace Equipment Div.; Bishop, G [British Aerospace (United Kingdom). Sowerby Research Centre; Lindermeir, E [Deutsche Forschungsanstalt fuer Luft- und Raumfahrt e. V. (DLR), Bonn (Germany). Inst. fuer Optoelektronik; Lister, D H [Defence and Research Agency, Hants (United Kingdom). Propulsion and Development Dept.; Wiesen, P [Bergische Univ. Wuppertal (Gesamthochshule) (Germany); Hilton, M [University of Reading (United Kingdom). Dept. of Physics

1998-12-31

The main goal of the AEROJET programme is to demonstrate the equivalence of remote measurement techniques to conventional extractive methods for both gaseous and particulate measurements. The different remote measurement techniques are compared and calibrated. A demonstrator measurement system for exhaust gases, temperature and particulates including data-analysis software is regarded as result of this project. Non-intrusive measurements are the method of choice within the AEROJET project promising to avoid the disadvantages of the gas sampling techniques which are currently used. Different ground based non-intrusive measurement methods are demonstrated during a final evaluation phase. Several non-intrusive techniques are compared with conventional gas sampling and analysis techniques. (R.P.) 3 refs.

β-Decay half-lives and nuclear structure of exotic proton-rich waiting point nuclei under rp-process conditions

Science.gov (United States)

Nabi, Jameel-Un; Böyükata, Mahmut

2016-03-01

We investigate even-even nuclei in the A ∼ 70 mass region within the framework of the proton-neutron quasi-particle random phase approximation (pn-QRPA) and the interacting boson model-1 (IBM-1). Our work includes calculation of the energy spectra and the potential energy surfaces V (β , γ) of Zn, Ge, Se, Kr and Sr nuclei with the same proton and neutron number, N = Z. The parametrization of the IBM-1 Hamiltonian was performed for the calculation of the energy levels in the ground state bands. Geometric shape of the nuclei was predicted by plotting the potential energy surfaces V (β , γ) obtained from the IBM-1 Hamiltonian in the classical limit. The pn-QRPA model was later used to compute half-lives of the neutron-deficient nuclei which were found to be in very good agreement with the measured ones. The pn-QRPA model was also used to calculate the Gamow-Teller strength distributions and was found to be in decent agreement with the measured data. We further calculate the electron capture and positron decay rates for these N = Z waiting point (WP) nuclei in the stellar environment employing the pn-QRPA model. For the rp-process conditions, our total weak rates are within a factor two compared with the Skyrme HF +BCS +QRPA calculation. All calculated electron capture rates are comparable to the competing positron decay rates under rp-process conditions. Our study confirms the finding that electron capture rates form an integral part of the weak rates under rp-process conditions and should not be neglected in the nuclear network calculations.

Evaluation and standardization of neutron activation analysis according to the K{sub 0} method in the RP-10 reactor; Evaluacion y estandarizacion del analisis por activacion neutronica segun el metodo del K{sub 0} en el reactor nuclear RP-10

Energy Technology Data Exchange (ETDEWEB)

Montoya R, E

1995-06-01

It has been characterized and standardized an irradiation of the RP-10 Research Nuclear Reactor for use of the K{sub 0} method of neutron activation analysis using the Hoegdahl convention; also it has been evaluate the behaviour of such method in regard to the accuracy and precision of the results obtained in the quantitative multi elemental analysis of several certified materials of reference. In order to prove that the analytical method is totally under statistical control, it has been used the Heydorn method. It has been verified that the method is exact, precise and reliable to determine the aluminium, antimuonium, arsenic, bromine, calcium, chloride, copper, magnesium, manganese, sodium, titanium, vanadium, zinc and other elements. Also, they are discussed, in regard to the use of K{sub 0} constants, the different formalisms employed to calculate the integral of the reaction rate by nucleus in the activation. (author). 58 refs., 18 tabs., 6 figs.

A High Redox Potential Laccase from Pycnoporus sanguineus RP15: Potential Application for Dye Decolorization

Directory of Open Access Journals (Sweden)

Ana L. R. L. Zimbardi

2016-05-01

Full Text Available Laccase production by Pycnoporus sanguineus RP15 grown in wheat bran and corncob under solid-state fermentation was optimized by response surface methodology using a Central Composite Rotational Design. A laccase (Lacps1 was purified and characterized and the potential of the pure Lacps1 and the crude culture extract for synthetic dye decolorization was evaluated. At optimal conditions (eight days, 26 °C, 18% (w/w milled corncob, 0.8% (w/w NH4Cl and 50 mmol·L−1 CuSO4, initial moisture 4.1 mL·g−1, the laccase activity reached 138.6 ± 13.2 U·g−1. Lacps1 was a monomeric glycoprotein (67 kDa, 24% carbohydrate. Optimum pH and temperature for the oxidation of 2,2’-azino-bis(3-ethylbenzthiazoline-6-sulfonate (ABTS were 4.4 and 74.4 °C, respectively. Lacps1 was stable at pH 3.0–8.0, and after two hours at 55–60 °C, presenting high redox potential (0.747 V vs. NHE. ABTS was oxidized with an apparent affinity constant of 147.0 ± 6.4 μmol·L−1, maximum velocity of 413.4 ± 21.2 U·mg−1 and catalytic efficiency of 3140.1 ± 149.6 L·mmol−1·s−1. The maximum decolorization percentages of bromophenol blue (BPB, remazol brilliant blue R and reactive blue 4 (RB4, at 25 or 40 °C without redox mediators, reached 90%, 80% and 60%, respectively, using either pure Lacps1 or the crude extract. This is the first study of the decolorization of BPB and RB4 by a P. sanguineus laccase. The data suggested good potential for treatment of industrial dye-containing effluents.

Antioxidant, Biomolecule Oxidation Protective Activities of Nardostachys jatamansi DC and Its Phytochemical Analysis by RP-HPLC and GC-MS

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Sakina Razack

2015-03-01

Full Text Available The study aimed at analyzing the metabolite profile of Nardostachys jatamansi using RP-HPLC, GC-MS and also its antioxidant, biomolecule protective and cytoprotective properties. The 70% ethanolic extract of Nardostachys jatamansi (NJE showed the presence of polyphenols and flavonoids (gallic acid, catechin, chlorogenic acid, homovanillin, epicatechin, rutin hydrate and quercetin-3-rhamnoside analyzed by RP-HPLC, whereas hexane extract revealed an array of metabolites (fatty acids, sesquiterpenes, alkane hydrocarbons and esters by GC-MS analysis. The antioxidant assays showed the enhanced potency of NJE with a half maximal inhibitory concentration (IC50 value of 222.22 ± 7.4 μg/mL for 2,2-diphenyl-1-picrylhydrazyl (DPPH, 13.90 ± 0.5 μg/mL for 2,2′-azino-bis(3-ethyl benzothiazoline-6-sulfonic acid diammonium salt (ABTS, 113.81 ± 4.2 μg/mL for superoxide, 948 ± 21.1 μg/mL for metal chelating and 12.3 ± 0.43 mg FeSO4 equivalent/g of extract for ferric reducing antioxidant power assays and was more potent than hexane extract. NJE effectively inhibited 2,2′-azobis(2-methylpropionamidine dihydrochloride (AAPH-induced oxidation of biomolecules analyzed by pBR322 plasmid DNA damage, protein oxidation of bovine serum albumin and lipid peroxidation assays. The observed effects might be due to the high content of polyphenols, 53.06 ± 2.2 mg gallic acid equivalents/g, and flavonoids, 25.303 ± 0.9 mg catechin equivalents/g, of NJE compared to the hexane fraction. Additionally, the extract abrogated the protein, carbonyl, and ROS formation, and NJE showed cytotoxicity in SH-SY5Y neuronal cells above 75 μg/mL. Thus, the study suggests that the herb unequivocally is a potential source of antioxidants and could aid in alleviating oxidative stress-mediated disorders.

A Validated RP-HPLC Method for Simultaneous Estimation of Atenolol and Indapamide in Pharmaceutical Formulations

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G. Tulja Rani

2011-01-01

Full Text Available A simple, fast, precise, selective and accurate RP-HPLC method was developed and validated for the simultaneous determination of atenolol and indapamide from bulk and formulations. Chromatographic separation was achieved isocratically on a Waters C18 column (250×4.6 mm, 5 µ particle size using a mobile phase, methanol and water (adjusted to pH 2.7 with 1% orthophosphoric acid in the ratio of 80:20. The flow rate was 1 mL/min and effluent was detected at 230 nm. The retention time of atenolol and indapamide were 1.766 min and 3.407 min. respectively. Linearity was observed in the concentration range of 12.5-150 µg/mL for atenolol and 0.625-7.5 µg/mL for indapamide. Percent recoveries obtained for both the drugs were 99.74-100.06% and 98.65-99.98% respectively. The method was validated according to the ICH guidelines with respect to specificity, linearity, accuracy, precision and robustness. The method developed can be used for the routine analysis of atenolol and indapamide from their combined dosage form.

Reduced Young modulus and hardness of electron irradiated binarypotassium-silicate glass

Czech Academy of Sciences Publication Activity Database

Gedeon, O.; Lukeš, J.; Jurek, Karel

2012-01-01

Roč. 275, MAR (2012), s. 7-10 ISSN 0168-583X R&D Projects: GA ČR GA104/09/1269 Institutional research plan: CEZ:AV0Z10100521 Keywords : electron radiation * silicate glass * mechanical properties Subject RIV: BM - Solid Matter Physics ; Magnetism Impact factor: 1.266, year: 2012

Molecular current modulator consisting of conjugated polymer chain with chemically attached photoactive side groups

Czech Academy of Sciences Publication Activity Database

Nešpůrek, Stanislav; Sworakowski, J.

2001-01-01

Roč. 393, 1-2 (2001), s. 168-176 ISSN 0040-6090 R&D Projects: GA AV ČR IAA1050901; GA AV ČR KSK4050111 Institutional research plan: CEZ:AV0Z4050913 Keywords : molecular electronics * photoconductivity * polymers Subject RIV: CD - Macromolecular Chemistry Impact factor: 1.266, year: 2001

Faktor pada perilaku Ibu dalam pemberian MPASI anak 6–24 bulan di Puskesmas Perumnas, Kendari

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Rosnah .

2016-03-01

.22.Conclusion: The result of multivariate analysis showed that the factor most dominantly associated with the behavior inthe supply of complementary breastfeeding was education of mothers (p=0.012; OR=5,40.KEYWORDS: behavior, complementary breastfeeding, infants of 6 – 24 monthsABSTRAKLatar Belakang: Masalah gangguan pertumbuhan balita di Indonesia berkaitan dengan banyaknya bayi yang sudahdiberi makanan pendamping ASI (MPASI sejak usia kurang dari atau 1 bulan. Hal ini mengakibatkan prevalensi gizikurang dan buruk di Kecamatan Kadia cukup tinggi. Masalah gizi ini dipengaruhi oleh tingkat kemampuan menyediakanpangan sesuai dengan kebutuhan anggota keluarga, pengetahuan dan perilaku keluarga dalam memilih, mengolah,dan membagi makanan di tingkat rumah tangga. Permasalahan lain kurangnya perhatian ibu untuk memantau tumbuhkembang balitanya.Tujuan:  Mengetahui faktor-faktor yang berhubungan dengan perilaku ibu dalam pemberian MPASI pada anak usia6-24 bulan di Puskesmas Perumnas Kecamatan Kadia, Kota Kendari.Metode: Jenis penelitian ini adalah analitik dengan rancangan cross sectional, menggunakan metode kuantitatif dan kualitatif. Subjek adalah ibu balita yang memenuhi kriteria inklusi di wilayah kerja Puskesmas Perumnas KecamatanKadia, Kota Kendari. Jumlah sampel sebanyak 97 subjek. Pengambilan sampel secara simple random sampling. Datadikumpulkan dengan panduan wawancara mendalam dan dianalisis dengan Chi-Square dan uji regresi logistik berganda.Data kualitatif dianalisis secara manual dan disajikan dalam bentuk narasi.Hasil: Dari faktor-faktor predisposisi, yang mempunyai hubungan bermakna dengan perilaku ibu dalam pemberian MPASIadalah pendidikan ibu (p=0,025; RP = 3,27, pendapatan per kapita keluarga (p=0,007; RP = 4,65, pengetahuan ibu(p= 0,05; RP= 2,75, dan sikap ibu (p = 0,027; RP=2,92 dengan perilaku pemberian MPASI. Dari faktor pendukung,partisipasi ibu ke posyandu mempunyai hubungan bermakna dengan perilaku pemberian MPASI (p=0,008; RP=3,65,sedangkan dari faktor

Olfactory bulb proteins linked to olfactory memory in C57BL/6J mice.

Science.gov (United States)

Li, Lin; Mauric, Veronika; Zheng, Jun-Fang; Kang, Sung Ung; Patil, Sudarshan; Höger, Harald; Lubec, Gert

2010-08-01

Information on systematic analysis of olfactory memory-related proteins is poor. In this study, the odor discrimination task to investigate olfactory recognition memory of adult male C57BL/6J mice was used. Subsequently, olfactory bulbs (OBs) were taken, proteins extracted, and run on two-dimensional gel electrophoresis with in-gel-protein digestion, followed by mass spectrometry and quantification of differentially expressed proteins. Dual specificity mitogen-activated protein kinase kinase 1 (MEK1), dihydropyrimidinase-related protein 1 (DRP1), and fascin are related with Lemon odor memory. Microtubule-associated protein RP/EB family member 3 is related to Rose odor memory. Hypoxanthine-guanine phosphoribosyltransferase is related with both Lemon and Rose odors memory. MEK1 and DRP1 levels were increased, while microtubule-associated protein RP/EB family member 3, fascin and hypoxanthine-guanine phosphoribosyltransferase levels were decreased during olfactory memory. In summary, neurogenesis, signal transduction, cytoskeleton, and nucleotide metabolism are involved in olfactory memory formation and storage of C57BL/6J mice.

A Study of Method Development, Validation, and Forced Degradation for Simultaneous Quantification of Paracetamol and Ibuprofen in Pharmaceutical Dosage Form by RP-HPLC Method

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Md. Sarowar Jahan

2014-01-01

Full Text Available A rapid and stability-indicating reversed phase high-performance liquid chromatography (RP-HPLC method was developed for simultaneous quantification of paracetamol and ibuprofen in their combined dosage form especially to get some more advantages over other methods already developed for this combination. The method was validated according to United States Pharmacopeia (USP guideline with respect to accuracy, precision, specificity, linearity, solution stability, robustness, sensitivity, and system suitability. Forced degradation study was validated according to International Conference on Harmonisation (ICH. For this, an isocratic condition of mobile phase comprising phosphate buffer (pH 6.8 and acetonitrile in a ratio of 65:35, v/v at a flow rate of 0.7 mL/minute over RP C18 (octadecylsilane (ODS, 150 × 4.6 mm, 5 μm, Phenomenex Inc. column at ambient temperature was maintained. The method showed excellent linear response with correlation coefficient ( R 2 values of 0.999 and 1.0 for paracetamol and ibuprofen respectively, which were within the limit of correlation coefficient ( R 2 > 0.995. The percent recoveries for two drugs were found within the acceptance limit of (97.0-103.0%. Intra-and inter-day precision studies of the new method were less than the maximum allowable limit percentage of relative standard deviation (%RSD ≤ 2.0. Forced degradation of the drug product was carried out as per the ICH guidelines with a view to establishing the stability-indicating property of this method and providing useful information about the degradation pathways, degradation products, and how the quality of a drug substance and drug product changes with time under the influence of various stressing conditions. The degradation of ibuprofen was within the limit (5-20%, according to the guideline of ICH, while paracetamol showed <20% degradation in oxidation and basic condition.

A Study of Method Development, Validation, and Forced Degradation for Simultaneous Quantification of Paracetamol and Ibuprofen in Pharmaceutical Dosage Form by RP-HPLC Method.

Science.gov (United States)

Jahan, Md Sarowar; Islam, Md Jahirul; Begum, Rehana; Kayesh, Ruhul; Rahman, Asma

2014-01-01

A rapid and stability-indicating reversed phase high-performance liquid chromatography (RP-HPLC) method was developed for simultaneous quantification of paracetamol and ibuprofen in their combined dosage form especially to get some more advantages over other methods already developed for this combination. The method was validated according to United States Pharmacopeia (USP) guideline with respect to accuracy, precision, specificity, linearity, solution stability, robustness, sensitivity, and system suitability. Forced degradation study was validated according to International Conference on Harmonisation (ICH). For this, an isocratic condition of mobile phase comprising phosphate buffer (pH 6.8) and acetonitrile in a ratio of 65:35, v/v at a flow rate of 0.7 mL/minute over RP C18 (octadecylsilane (ODS), 150 × 4.6 mm, 5 μm, Phenomenex Inc.) column at ambient temperature was maintained. The method showed excellent linear response with correlation coefficient (R (2)) values of 0.999 and 1.0 for paracetamol and ibuprofen respectively, which were within the limit of correlation coefficient (R (2) > 0.995). The percent recoveries for two drugs were found within the acceptance limit of (97.0-103.0%). Intra-and inter-day precision studies of the new method were less than the maximum allowable limit percentage of relative standard deviation (%RSD) ≤ 2.0. Forced degradation of the drug product was carried out as per the ICH guidelines with a view to establishing the stability-indicating property of this method and providing useful information about the degradation pathways, degradation products, and how the quality of a drug substance and drug product changes with time under the influence of various stressing conditions. The degradation of ibuprofen was within the limit (5-20%, according to the guideline of ICH), while paracetamol showed degradation in oxidation and basic condition.

Involvement of CD36 in Modulating the Decrease of NPY and AgRP Induced by Acute Palmitic Acid Stimulation in N1E-115 Cells

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Yan Ma

2017-06-01

Full Text Available Central nervous system (CNS fatty acid sensing plays an important role in the regulation of food intake, and palmitic acid (PA is the most important long chain fatty acid (LCFA in the mammalian diet. To explore the effect of PA on central neuropeptide expression and the role of the cluster of the differentiation of 36 (CD36 in the process, N1E-115 cells were cultured with PA in the presence or absence of sulfosuccinimidyl-oleate (SSO, a CD36 inhibitor. Results showed that 10 μmol/L PA significantly reduced NPY and AgRP mRNA expression after 20 min of exposure, while the expression of CD36 was upregulated. The presence of SSO significantly attenuated the decrease of NPY and AgRP expression that was induced by PA alone, although no notable effect on PA- induced CD36 gene expression was observed. In conclusion, our study suggests the involvement of CD36 in the PA-induced decrease of NPY and AgRP in N1E-115 cells.

New Stability Indicating RP-HPLC Method for the Estimation of Cefpirome Sulphate in Bulk and Pharmaceutical Dosage Forms.

Science.gov (United States)

Rao, Kareti Srinivasa; Kumar, Keshar Nargesh; Joydeep, Datta

2011-01-01

A simple stability indicating reversed-phase HPLC method was developed and subsequently validated for estimation of Cefpirome sulphate (CPS) present in pharmaceutical dosage forms. The proposed RP-HPLC method utilizes a LiChroCART-Lichrosphere100, C18 RP column (250 mm × 4mm × 5 μm) in an isocratic separation mode with mobile phase consisting of methanol and water in the proportion of 50:50 % (v/v), at a flow rate 1ml/min, and the effluent was monitored at 270 nm. The retention time of CPS was 2.733 min and its formulation was exposed to acidic, alkaline, photolytic, thermal and oxidative stress conditions, and the stressed samples were analyzed by the proposed method. The described method was linear over a range of 0.5-200μg/ml. The percentage recovery was 99.46. F-test and t-test at 95% confidence level were used to check the intermediate precision data obtained under different experimental setups; the calculated value was found to be less than the critical value.

PMP Estimations at Sparsely Controlled Andinian Basins and Climate Change Projections

Science.gov (United States)

Lagos Zúñiga, M. A.; Vargas, X.

2012-12-01

average liquid precipitation over the basin. Temperature projections would also affect the maximization factors in the calculation of the PMP, increasing it up to 126.6% and 62.5% in scenarios A2 and B1, respectively. These projections are important to be studied due to the implications of PMP in hydrologic design of great hydraulic works as Probable Maximum Flood (PMF). We propose that the methodology presented in this study could be also used in other basins of similar characteristics.

Energy Balance Regulating Neuropeptides Are Expressed through Pregnancy and Regulated by Interleukin-6 Deficiency in Mouse Placenta.

Science.gov (United States)

Pazos, Patricia; Lima, Luis; Diéguez, Carlos; García, María C

2014-01-01

The placenta produces a number of signaling molecules including metabolic and reproductive hormones as well as several inflammatory mediators. Among them, Interleukin-6 (IL-6), a well-known immune and metabolic regulator, acts peripherally modulating metabolic function and centrally increasing energy expenditure and reducing body fat. IL-6 interacts with key hypothalamic neuropeptidergic systems controlling energy homeostasis such as those producing the orexigenic/anabolic: neuropeptide Y (NPY) and agouti-related peptide (AgRP) and anorectic/catabolic neuropeptides: proopiomelanocortin (POMC) and cocaine and amphetamine regulated transcript (CART). Human and rat placenta have been identified as source of these neuropeptides, but their expression and regulation in murine placental tissues remain unknown. Therefore, placental mRNA levels of IL-6, NPY, AgRP, POMC, and CART at different pregnancy stages (gestational days 13, 15, and 18) were analyzed by real time PCR, as were the effect of IL-6 deficiency (IL-6 knockout mice) on their placental expression. Our results showed that placenta-derived neuropeptides were regulated by gestational age and IL-6 throughout the second half of mouse pregnancy. These data suggest that IL-6 may participate in the fine tune control of energy balance during pregnancy by extending its action as a metabolic signal to the main organ at the fetomaternal interface: the placenta.

[ERG diagnosis and differential diagnosis: results of examination over 6 years].

Science.gov (United States)

Stemeyer, G; Stähli, P

1996-05-01

This study reviews the patient material first from the point of view of referral diagnosis. Secondly, we focus on difficulties in selective differential diagnoses. 1501 patients underwent electroretinographic (ERG) testing from 1989 to 1994, amounting to 1815 ERG recordings, including follow-up examinations. The technique applied is full-field, single flash ERG with selective stimulation of the rod- and of the cone-systems. In 3.8% (57 cases) the ERG was performed under general anesthesia in outpatients. Tapetoretinal degenerations, toxic retinal side effects, inflammatory disease and ocular trauma represented, in this order, the major groups of referral diagnoses aside from unclear visual loss. The documentation or the exclusion of tapetoretinal degeneration represented the largest share (57%) of the application of the diagnostic procedure. 171 cases of isolated retinitis pigmentosa (RP) and 33 cases of syndromic RP were identified. Frequent and rare diagnostic entities and their differential diagnoses within this group are discussed. Inevitably, a number of diagnostic decisions remain problematic, in particular at the first examination. These diagnostic difficulties are addressed also and include the differentiation between RP sine pigmento and congenital amaurosis Leber in infants, RP with macular involvement vs. cone-rod degeneration, unilateral RP vs. postinflammatory conditions, and progressive cone dystrophy vs. achromatopsia, cone-rod degeneration or Stargardt's disease. Frequent and meaningful indications for ERG recording and difficult diagnostic decisions arise from this review of a relatively large group of patients. A number of diagnoses can hardly, if not at all be established without ERG testing. These include retinal cause of visual loss in infants, congenital amaurosis Leber, RP sine pigmento, early stages of RP, carrier status in XL RP and in choroideremia, progressive cone dystrophy, toxic retinopathy without fundus changes, retinal involvement

CeB6 electron gun for the soft X-ray FEL project at SPring-8

International Nuclear Information System (INIS)

Togawa, K.; Baba, H.; Onoe, K.; Inagaki, T.; Shintake, T.; Matsumoto, H.

2004-01-01

A pulsed high-voltage electron gun with a thermionic cathode is under development for the injector system of the soft X-ray FEL project at SPring-8 (SCSS project). A CeB 6 single crystal of 3 mm diameter was chosen as a thermionic cathode because of its excellent emission properties, i.e., high resistance against contamination, uniform emission density and smooth surface. The CeB 6 cathode can produce a 3 A beam with 2 μs FWHM. A gun voltage of -500 kV was chosen as a compromise between the need for controlling emittance growth and minimizing the risks of high-voltage arcing. We have constructed a 500 kV electron gun test stand and have begun performance tests. This paper describes the basic design and the current status of the hardware R and D on the CeB 6 gun

A desk evaluation review of project MYA/6/015 hormonal studies on pituitary adrenal disorders. Project desk evaluation

International Nuclear Information System (INIS)

1994-01-01

Snake-bite is one of the high priority health problems in Myanmar, causing high mortality and mobility rates among the rural population. In 1982 the Department of Medical Research (DMR) of the Ministry of Health launched a research programme for the comprehensive studies of snake (Russell's viper) bites and since then various research divisions of the DMR have been actively engaged in both basic and clinical studies aimed at obtaining adequate knowledge of the biochemical, clinical and pathophysiological mechanisms to guide rational management of snake-bite cases. Also, a clinical research unit has been established in a particular area where the incidence of Russell's viper bite is quite high. Project MYA/6/015 was initiated in 1989 with the objective of determining the incidence, facilitating early diagnosis and assessing the value of therapy in hypopituitarism following snake bite, through the measurements of pituitary and adreno-cortical hormones in serum and their changes in response to dynamic tests. The project was completed in July 1993. The review was undertaken upon request by the Asia and Pacific Section, to assess the overall performance of the project

A desk evaluation review of project MYA/6/015 hormonal studies on pituitary adrenal disorders. Project desk evaluation

Energy Technology Data Exchange (ETDEWEB)

NONE

1994-03-09

Snake-bite is one of the high priority health problems in Myanmar, causing high mortality and mobility rates among the rural population. In 1982 the Department of Medical Research (DMR) of the Ministry of Health launched a research programme for the comprehensive studies of snake (Russell`s viper) bites and since then various research divisions of the DMR have been actively engaged in both basic and clinical studies aimed at obtaining adequate knowledge of the biochemical, clinical and pathophysiological mechanisms to guide rational management of snake-bite cases. Also, a clinical research unit has been established in a particular area where the incidence of Russell`s viper bite is quite high. Project MYA/6/015 was initiated in 1989 with the objective of determining the incidence, facilitating early diagnosis and assessing the value of therapy in hypopituitarism following snake bite, through the measurements of pituitary and adreno-cortical hormones in serum and their changes in response to dynamic tests. The project was completed in July 1993. The review was undertaken upon request by the Asia and Pacific Section, to assess the overall performance of the project.

GMMIP (v1.0) contribution to CMIP6: Global Monsoons Model Inter-comparison Project

Energy Technology Data Exchange (ETDEWEB)

Zhou, Tianjun; Turner, Andrew G.; Kinter, James L.; Wang, Bin; Qian, Yun; Chen, Xiaolong; Wu, Bo; Wang, Bin; Liu, Bo; Zou, Liwei; He, Bian

2016-10-10

The Global Monsoons Model Inter-comparison Project (GMMIP) has been endorsed by the panel of Coupled Model Inter-comparison Project (CMIP) as one of the participating model inter-comparison projects (MIPs) in the sixth phase of CMIP (CMIP6). The focus of GMMIP is on monsoon climatology, variability, prediction and projection, which is relevant to four of the “Grand Challenges” proposed by the World Climate Research Programme. At present, 21 international modeling groups are committed to joining GMMIP. This overview paper introduces the motivation behind GMMIP and the scientific questions it intends to answer. Three tiers of experiments, of decreasing priority, are designed to examine (a) model skill in simulating the climatology and interannual-to-multidecadal variability of global monsoons forced by the sea surface temperature during historical climate period; (b) the roles of the Interdecadal Pacific Oscillation and Atlantic Multidecadal Oscillation in driving variations of the global and regional monsoons; and (c) the effects of large orographic terrain on the establishment of the monsoons. The outputs of the CMIP6 Diagnostic, Evaluation and Characterization of Klima experiments (DECK), “historical” simulation and endorsed MIPs will also be used in the diagnostic analysis of GMMIP to give a comprehensive understanding of the roles played by different external forcings, potential improvements in the simulation of monsoon rainfall at high resolution and reproducibility at decadal timescales. The implementation of GMMIP will improve our understanding of the fundamental physics of changes in the global and regional monsoons over the past 140 years and ultimately benefit monsoons prediction and projection in the current century.

Determination of n-octanol/water partition coefficient for DDT-related compounds by RP-HPLC with a novel dual-point retention time correction.

Science.gov (United States)

Han, Shu-ying; Qiao, Jun-qin; Zhang, Yun-yang; Yang, Li-li; Lian, Hong-zhen; Ge, Xin; Chen, Hong-yuan

2011-03-01

n-Octanol/water partition coefficients (P) for DDTs and dicofol were determined by reversed-phase high performance liquid chromatography (RP-HPLC) on a C(18) column using methanol-water mixture as mobile phase. A dual-point retention time correction (DP-RTC) was proposed to rectify chromatographic retention time (t(R)) shift resulted from stationary phase aging. Based on this correction, the relationship between logP and logk(w), the logarithm of the retention factor extrapolated to pure water, was investigated for a set of 12 benzene homologues and DDT-related compounds with reliable experimental P as model compounds. A linear regression logP=(1.10±0.04) logk(w) - (0.60±0.17) was established with correlation coefficient R(2) of 0.988, cross-validated correlation coefficient R(cv)(2) of 0.983 and standard deviation (SD) of 0.156. This model was further validated using four verification compounds, naphthalene, biphenyl, 2,2-bis(4-chlorophenyl)-1,1-dichloroethane (p,p'-DDD) and 2,2-bis(4-chlorophenyl)-1,1-dichloroethene (p,p'-DDE) with similar structure to DDT. The RP-HPLC-determined P values showed good consistency with shake-flask (SFM) or slow-stirring (SSM) results, especially for highly hydrophobic compounds with logP in the range of 4-7. Then, the P values for five DDT-related compounds, 2-(2-chlorophenyl)-2-(4-chlorophenyl)-1,1,1-trichloroethane (o,p'-DDT), 2-(2-chlorophenyl)-2-(4-chlorophenyl)-1,1-dichloroethane (o,p'-DDD), 2-(2-chlorophenyl)-2-(4-chlorophenyl)-1,1-dichloroethene (o,p'-DDE), and 2,2,2-trichloro-1,1-bis(4-chlorophenyl)ethanol (dicofol) and its main degradation product 4,4'-dichlorobenzophenone (p,p'-DBP) were evaluated by the improved RP-HPLC method for the first time. The excellent precision with SD less than 0.03 proved that the novel DP-RTC protocol can significantly increases the determination accuracy and reliability of P by RP-HPLC. Copyright © 2011 Elsevier Ltd. All rights reserved.

Research

African Journals Online (AJOL)

abp

15 sept. 2017 ... Introduction: Chronic kidney disease is fast becoming a worldwide public health problem due to the increase of ... Les projections de 2030 prévoient que plus de 70% de la population ..... the global burden of major noncommunicable diseases. ... 2002 Feb; 39(2 Suppl 1):S1-266. ... Risk of death and the.

Experimental studies on the development of Contracaecum rudolphii (Nematoda: Anisakidae) in copepod and fish paratenic hosts

Czech Academy of Sciences Publication Activity Database

Moravec, František

2009-01-01

Roč. 56, č. 3 (2009), s. 185-193 ISSN 0015-5683 R&D Projects: GA MŠk LC522; GA AV ČR IA62211 Institutional research plan: CEZ:AV0Z60220518 Keywords : Contracaecum rudolphii * life cycle * Czech Republic Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 1.266, year: 2009

Goezia spinulosa (Nematoda: Raphidascarididae), a pathogenic parasite of the arapaima Arapaima gigas (Osteichthyes)

Czech Academy of Sciences Publication Activity Database

Santos, C. P.; Moravec, František

2009-01-01

Roč. 56, č. 1 (2009), s. 55-63 ISSN 0015-5683 R&D Projects: GA MŠk LC522; GA ČR(CZ) GA524/06/0170 Institutional research plan: CEZ:AV0Z60220518 Keywords : Goezia * Arapaima * Brazil Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 1.266, year: 2009

Reaching the superlinear convergence phase of the CG method

Czech Academy of Sciences Publication Activity Database

Axelsson, Owe; Karátson, J.

2014-01-01

Roč. 260, č. 260 (2014), s. 244-257 ISSN 0377-0427 R&D Projects: GA MŠk ED1.1.00/02.0070 Institutional support: RVO:68145535 Keywords : superlinear convergence * conjugate gradient method * eigenvalues Subject RIV: BA - General Mathematics Impact factor: 1.266, year: 2014 http://www.sciencedirect.com/science/article/pii/S0377042713005451

Three-dimensional seismic velocity model of theWest Bohemia/Vogtland seismoactive region

Czech Academy of Sciences Publication Activity Database

Růžek, Bohuslav; Horálek, Josef

2013-01-01

Roč. 195, č. 2 (2013), s. 1251-1266 ISSN 0956-540X R&D Projects: GA ČR GAP210/12/2336; GA MŠk LM2010008 Institutional support: RVO:67985530 Keywords : controlled source seismology * body waves * seismic tomography * crustal structure Subject RIV: DC - Siesmology, Volcanology, Earth Structure Impact factor: 2.724, year: 2013

Detection of a Rickettsia Closely Related to Rickettsia aeschlimannii, “Rickettsia heilongjiangensis,” Rickettsia sp. Strain RpA4, and Ehrlichia muris in Ticks Collected in Russia and Kazakhstan

OpenAIRE

Shpynov, Stanislav; Fournier, Pierre-Edouard; Rudakov, Nikolay; Tankibaev, Marat; Tarasevich, Irina; Raoult, Didier

2004-01-01

Using PCR, we screened 411 ticks from four genera collected in Russia and Kazakhstan for the presence of rickettsiae and ehrlichiae. In Russia, we detected “Rickettsia heilongjiangensis,” Rickettsia sp. strain RpA4, and Ehrlichia muris. In Kazakhstan, we detected Rickettsia sp. strain RpA4 and a rickettsia closely related to Rickettsia aeschlimannii. These agents should be considered in a differential diagnosis of tick-borne infections in these areas.

Stability of [6]-gingerol and [6]-shogaol in simulated gastric and intestinal fluids.

Science.gov (United States)

Bhattarai, Sushila; Tran, Van H; Duke, Colin C

2007-11-30

The degradation kinetics of [6]-gingerol and [6]-shogaol were investigated in simulated gastric (pH 1) and intestinal (pH 7.4) fluids at 37 degrees C. Degradation products were quantitatively determined by HPLC (Lichrospher 60 RP select B column, 5 microm, 125 mm x 4 mm; mobile phase: methanol-water-acetic acid (60:39:1 v/v); flow rate: 0.6 ml/min; detection UV: 280 nm). In simulated gastric fluid (SGF) [6]-gingerol and [6]-shogaol underwent first-order reversible dehydration and hydration reactions to form [6]-shogaol and [6]-gingerol, respectively. The degradation was catalyzed by hydrogen ions and reached equilibrium at approximately 200 h. In simulated intestinal fluid (SIF) both [6]-gingerol and [6]-shogaol showed insignificant interconversion between one another. Addition of amino acids glycine, 3-amino propionic acid (beta-alanine) and gamma-amino butyric acid (GABA), and ammonium acetate at a range of concentrations of 0.05-0.5mM had no effect on the rate of degradation of [6]-shogaol in SGF and 0.1M HCl solution. However, at exceedingly high concentration (0.5M) of ammonium acetate and glycine, significant amounts of [6]-shogaol ammonia and glycine adducts were detected. The degradation profile of [6]-gingerol and [6]-shogaol under simulated physiological conditions reported in this study will provide insight into the stability of these compounds when administered orally.

Dicty_cDB: Contig-U04374-1 [Dicty_cDB

Lifescience Database Archive (English)

Full Text Available 1 Anolis sagre... 46 1.6 1 ( GE801267 ) EST_scau_evk_891626 scauevk mixed_tissue Sebastes... 46 1.6 1 ( GE80...1266 ) EST_scau_evk_892010 scauevk mixed_tissue Sebastes... 46 1.6 1 ( FG735927 ) G1145P337RE22.T0 Anolis ca

Specific in vivo binding in the rat brain of [18F]RP 62203: A selective 5-HT2A receptor radioligand for positron emission tomography

International Nuclear Information System (INIS)

Besret, Laurent; Dauphin, Francois; Huard, Cecile; Lasne, Marie-Claire; Vivet, Richard; Mickala, Patrick; Barbelivien, Alexandra; Baron, Jean-Claude

1996-01-01

In vivo pharmacokinetic and brain binding characteristics of [ 18 F]RP 62203, a selective high-affinity serotonergic 5-HT 2A receptor antagonist, were assessed in the rat following intravenous injection of trace amount of the radioligand. The radioactive distribution profile observed in the brain 60 min after injection was characterized by greater than fourfold higher uptake in neocortex as compared to cerebellum (0.38 ± 0.07% injected dose/g, % ID/g and 0.08 ± 0.01 ID/g, respectively), consistent with in vivo specific binding to the 5-HT 2A receptor. Furthermore, specific [ 18 F]RP 62203 binding significantly correlated with the reported in vitro distribution of 5-HT 2A receptors, but not with known concentration profiles of dopaminergic D 2 or adrenergic α 1 receptors. Finally, detectable specific binding was abolished by pretreatment with large doses of ritanserin, a selective 5-HT 2A antagonist, which resulted in uniform uptakes across cortical, striatal and cerebellar tissues. Thus, [ 18 F]RP 62203 appears to be a promising selective tool to visualize and quantify 5-HT 2A brain receptors in vivo with positron emission tomography

RP-HPLC determination of water-soluble vitamins in honey.

Science.gov (United States)

Ciulu, Marco; Solinas, Silvia; Floris, Ignazio; Panzanelli, Angelo; Pilo, Maria I; Piu, Paola C; Spano, Nadia; Sanna, Gavino

2011-01-15

The assessment and validation of reliable analytical methods for the determination of vitamins in sugar-based matrices (e.g. honey) are still scarcely explored fields of research. This study proposes and fully validates a simple and fast RP-HPLC method for the simultaneous determination of five water-soluble vitamins (vitamin B(2), riboflavin; vitamin B(3), nicotinic acid; vitamin B(5), pantothenic acid; vitamin B(9), folic acid; and vitamin C, ascorbic acid) in honey. The method provides low detection and quantification limits, very good linearity in a large concentration interval, very good precision, and the absence of any bias. It has been successfully applied to 28 honey samples (mainly from Sardinia, Italy) of 12 different botanical origins. While the overall amount of the analytes in the samples is quite low (always below 40 mg kg(-1)), we have observed a marked dependence of some of their concentrations (i.e. vitamin B(3) and vitamin B(5)) and the botanical origin of the honey. This insight might lead to important characterization features for this food item. Copyright © 2010 Elsevier B.V. All rights reserved.

Site-specific deletions of chromosomally located DNA segments with the multimer resolution system of broad-host-range plasmid RP4

DEFF Research Database (Denmark)

Sternberg, Claus; Eberl, Leo; Sanchezromero, Juan M.

1995-01-01

The multimer resolution system (mrs) of the broad-host-range plasmid RP4 has been exploited to develop a general method that permits the precise excision of chromosomal segments in a variety of gram-negative bacteria. The procedure is based on the site-specific recombination between two directly ...

Rg1 inhibits high glucose-induced mesenchymal activation and fibrosis via regulating miR-2113/RP11-982M15.8/Zeb1 pathway.

Science.gov (United States)

Xue, Li-Ping; Fu, Xiao-Lin; Hu, Min; Zhang, Li-Wei; Li, Ya-Di; Peng, Ya-Li; Ding, Peng

2018-07-02

Recent study has showed that Ginsenoside Rg1, the mian active compound of Panax ginseng, could ameliorate oxidative stress and myocardial apoptosis in diabetes mellitus. However, the roles and mechanisms of Rg1 in proliferative diabetic retinopathy (PDR) are still unclear. In the present study, we aimed to investigate the effects of Rg1 on mesenchymal activation of high-glucose (HG) cultured müller cells. High glucose conditions up-regulate MMP-2, MMP-9 and down-regulate TIMP-2, and promote mesenchymal activation in Müller cells. And Rg1 inhibits the HG-induced mesenchymal activation and HG-increased MMP-2 and MMP-9 and HG-decreased TIMP-2 in Müller cells. HG up-regulates Zeb1 and lncRNA RP11-982M15.8, and down-regulates miR-2113, and Rg1 inhibits these effects of HG. Both inhibition of miR-2113 and over-expression of RP11-982M15.8 significantly restored the HG induced mesenchymal activasion. Taken together, our findings suggested that Rg1 inhibited HG-induced mesenchymal activation and fibrosis via regulating miR-2113/RP11-982M15.8/Zeb1 pathway. Copyright © 2018. Published by Elsevier Inc.

Individual Phosphatidylcholine Species Analysis by RP-HPLC-ELSD for Determination of Polyenylphosphatidylcholine in Lecithins.

Science.gov (United States)

Lee, Wei-Ju; Weng, Shun-Hsiang; Su, Nan-Wei

2015-04-22

Polyenylphosphatidylcholine (PPC), a subgroup of the bioactive agents in phosphatidylcholine (PC), has been indicated to possess liver-protective effects. This study aimed to investigate a promising and feasible method to determine PC molecular species with a reverse phase (RP) high-performance liquid chromatograph (HPLC) equipped with an evaporative light scattering detector (ELSD). Chromatography was achieved using a C30 column and an isocratic mobile phase consisting of acetonitrile/methanol/triethylamine (40/58/2, v/v/v) at a flow rate of 1 mL/min, and ELSD detection was performed using 80 °C for the drift tube and an air flow rate of 1.8 L/min. To identify individual peaks on the chromatogram, MALDI-TOF-MS was employed for initial detection, and then the results were used to investigate the relationship between the retention time and fatty acyl chains of each PC molecule. A linear correlation was observed between the retention time and theoretical carbon number (TCN) of individual PC species. The compositions of PC molecular species in soybean and sunflower lecithins were similar to each other, and the major PC molecular species were 1,2-dilinoleoyl-sn-glycero-3-phosphocholine (LLPC), 1-oleoyl-2-linoleoyl-sn-glycero-3-phosphocholine (OLPC), and 1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine (PLPC). The contents of LLPC in soybean PC and sunflower PC were 40.6% and 64.3%, respectively.

D6.4 Implementation: Project Knowledge Management

DEFF Research Database (Denmark)

Dolog, Peter; Durao, Frederico; Jahn, Karsten

2009-01-01

This report presents the prototype for the project knowledge management in Logica. We describe the related theories and our approach, the ‘circle of knowledge’. According to our analysis it results in the data-based import or update process between two specialized applications, one for project...... management and one for knowledge sharing. We describe the applications involved and the features used. The main component here is a templating mechanism in the KiWi system....

12,12′-[2,2′-Oxybis(ethane-2,1-diylbis(oxy]bis[(Rp-4-bromo[2.2]paracyclophane

Directory of Open Access Journals (Sweden)

Bing Hong

2011-04-01

Full Text Available The title compound, C36H36Br2O3, was synthesized from (Rp-4-bromo-12-hydroxy[2.2]paracyclophane and oxydiethane-2,1-diyl bis(4-methylbenzenesulfonate. The crystal packing exhibits a short O...Br interaction [Br...O = 3.185 (3 Å] and a weak intermolecular C—H...O contact.

3.6-m Devasthal Optical Telescope Project: Completion and first results

Science.gov (United States)

Kumar, Brijesh; Omar, Amitesh; Maheswar, Gopinathan; Pandey, Anil Kumar; Sagar, Ram; Uddin, Wahab; Sanwal, Basant Ballabh; Bangia, Tarun; Kumar, Tripurari Satyanarayana; Yadav, Shobhit; Sahu, Sanjit; Pant, Jayshreekar; Reddy, Bheemireddy Krishna; Gupta, Alok Chandra; Chand, Hum; Pandey, Jeewan Chandra; Joshi, Mohit Kumar; Jaiswar, Mukeshkuma; Nanjappa, Nandish; Purushottam; Yadav, Rama Kant Singh; Sharma, Saurabh; Pandey, Shashi Bhushan; Joshi, Santosh; Joshi, Yogesh Chandra; Lata, Sneh; Mehdi, Biman Jyoti; Misra, Kuntal; Singh, Mahendra

2018-04-01

We present an update on the 3.6-m aperture optical telescope, which has been installed at Devasthal in the year 2016. In this paper, a brief overview of installation activities at site and first results are presented. The 3.6-m Devasthal Optical Telescope project was initiated in 2007 by the Aryabhatta Research Institute of Observational Sciences (ARIES; Nainital, India) in partnership with Belgium. The telescope has Ritchey-Chretien optics, an alt-azimuth mount, an active control of the primary and a corrected science field of view of 30' at the Cassegrain focus. The construction of the telescope enclosure building was completed in June 2014 and after successful installation of the telescope. The first engineering light was obtained on 22 March 2015. The on-sky performance of the telescope was carried out till February 2016.

LIFAC sorbent injection desulfurization demonstration project. Final report, volume II: Project performance and economics

Energy Technology Data Exchange (ETDEWEB)

NONE

1996-01-01

This publication discusses the demonstration of the LIFAC sorbent injection technology at Richmond Power and Light`s Whitewater Valley Unit No. 2, performed under the auspices of the U.S. Department of Energy`s (DOE) Clean Coal Technology Program. LIFAC is a sorbent injection technology capable of removing 75 to 85 percent of a power plant`s SO{sub 2} emissions using limestone at calcium to sulfur molar ratios of between 2 and 2.5 to 1. The site of the demonstration is a coal-fired electric utility power plant located in Richmond, Indiana. The project is being conducted by LIFAC North America (LIFAC NA), a joint venture partnership of Tampella Power Corporation and ICF Kaiser Engineers, in cooperation with DOE, RP&L, and Research Institute (EPRI), the State of Indiana, and Black Beauty Coal Company. The purpose of Public Design Report Volume 2: Project Performance and Economics is to consolidate, for public use, the technical efficiency and economy of the LIFAC Process. The report has been prepared pursuant to the Cooperative Agreement No. DE-FC22-90PC90548 between LIFAC NA and the U.S. Department of Energy.

Energy Balance Regulating Neuropeptides Are Expressed through Pregnancy and Regulated by Interleukin-6 Deficiency in Mouse Placenta

Directory of Open Access Journals (Sweden)

Patricia Pazos

2014-01-01

Full Text Available The placenta produces a number of signaling molecules including metabolic and reproductive hormones as well as several inflammatory mediators. Among them, Interleukin-6 (IL-6, a well-known immune and metabolic regulator, acts peripherally modulating metabolic function and centrally increasing energy expenditure and reducing body fat. IL-6 interacts with key hypothalamic neuropeptidergic systems controlling energy homeostasis such as those producing the orexigenic/anabolic: neuropeptide Y (NPY and agouti-related peptide (AgRP and anorectic/catabolic neuropeptides: proopiomelanocortin (POMC and cocaine and amphetamine regulated transcript (CART. Human and rat placenta have been identified as source of these neuropeptides, but their expression and regulation in murine placental tissues remain unknown. Therefore, placental mRNA levels of IL-6, NPY, AgRP, POMC, and CART at different pregnancy stages (gestational days 13, 15, and 18 were analyzed by real time PCR, as were the effect of IL-6 deficiency (IL-6 knockout mice on their placental expression. Our results showed that placenta-derived neuropeptides were regulated by gestational age and IL-6 throughout the second half of mouse pregnancy. These data suggest that IL-6 may participate in the fine tune control of energy balance during pregnancy by extending its action as a metabolic signal to the main organ at the fetomaternal interface: the placenta.

Intra-specific genetic relationship analyses of Elaeagnus angustifolia based on RP-HPLC biochemical markers

Institute of Scientific and Technical Information of China (English)

无

2006-01-01

Elaeagnus angustifolia Linn. has various ecological, medicinal and economical uses. An approach was established using RP-HPLC (reversed-phase high-performance liquid chromatography) to classify and analyse the intra-specific genetic relationships of seventeen populations of E. angustifolia, collected from the Xinjiang areas of China. Chromatograms of alcohol-soluble proteins produced by seventeen populations ofE. angustifolia, were compared. Each chromatogram of alcohol-soluble proteins came from a single seed of one wild plant only. The results showed that when using a Waters Delta Pak. C18, 5 μm particle size reversed phase column (150 mm×3.9 mm), a linear gradient of 25%～60% solvent B with flow rate of 1 ml/min and run time of 67 min, the chromatography yielded optimum separation ofE. angustifolia alcohol-soluble proteins. Representative peaks in each population were chosen according to peak area and occurrence in every seed. The converted data on the elution peaks of each population were different and could be used to represent those populations. GSC (genetic similarity coefficients) of 41% to 62% showed a medium degree of genetic diversity among the populations in these eco-areas. Cluster analysis showed that the seventeen populations ofE. angustifolia could be divided into six clusters at the GSC=0.535 level and indicated the general and unique biochemical markers of these clusters. We suggest that E. angustifolia distribution in these eco-areas could be classified into six variable species. RP-HPLC was shown to be a rapid, repeatable and reliable method for E. angustifolia classification and identification and for analysis of genetic diversity.

Stability-Indicating RP-HPLC Method for Assay of Silver Lactate

Directory of Open Access Journals (Sweden)

V. Srinivasan

2011-01-01

Full Text Available A simple, economic and time-efficient stability-indicating, reverse-phase high-performance liquid chromatographic (RP-HPLC method has been developed for analysis of silver lactate in the presence of degradation products generated by decomposition. When silver lactate was subjected to acid hydrolysis, base hydrolysis, oxidative, photolytic, humidity and thermal stress, degradation was observed during base hydrolysis, oxidation, humidity and thermal stress. The drug was found to be stable to other stress conditions. Successful chromatographic condition of the drug from the degradation products formed under stress conditions was achieved on a phenomenex Gemini column with potassium dihydrogen phosphate buffer, pH adjusted to 2.2 with orthophosphoric acid, as mobile phase. The method was validated for linearity, precision, specificity and robustness and can be used for quality-control during manufacture and assessment of the stability of samples of silver lactate. To the best of our knowledge, a validated stability-indicating LC assay method for silver lactate based on lactic acid is reported for the first time.

Parallel studies of His-DTrp-Ala-Trp-DPhe-Lys-NH2 and human pancreatic growth hormone-releasing factor-44-NH2 in rat primary pituitary cell monolayer culture.

Science.gov (United States)

Sartor, O; Bowers, C Y; Chang, D

1985-03-01

His-DTrp-Ala-Trp-DPhe-Lys-NH2 (GH-RP-6) is a synthetic hexapeptide that specifically releases GH both in vivo and in vitro in pituitary incubates. In this study, for the first time, GH-RP-6 was studied in primary pituitary cell monolayer culture. Parallel studies were performed with human pancreatic GH-releasing factor-44 (hpGRF-44). Culture conditions optimal for GH-RP-6 were not optimal for hpGRF-44. Both peptides released GH in a dose- and time-dependent manner. In this assay system, the ED50 for GH-RP-6 was 9 nM, and the ED50 for hp-GRF-44 was 1.6 nM. Calcium-blocking agents inhibited the GH responses of both peptides as well as basal GH release. Pretreatment with GH-RP-6 decreased the subsequent response to both GH-RP-6 and hpGRF-44. hpGRF-44 down regulated itself but not GH-RP-6. Rat sera potentiated the GH response of hpGRF-44 but not that of GH-RP-6. GH-RP-6 and hpGRF-44 GH responses were additive. These results suggest that GH-RP-6 and hpGRF-44 stimulate GH release via different somatotroph receptors.

Simultaneous determination of hyoscine N-butyl bromide and paracetamol in their binary mixture by RP-HPLC method

Directory of Open Access Journals (Sweden)

Nouruddin W. Ali

2017-05-01

Full Text Available RP-HPLC chromatographic method was developed for the determination of hyoscine N-butyl bromide (HBB and Paracetamol (PAR. In this chromatographic method, HBB and PAR were separated using C18 (25 cm × 4.6 mm i.d. 5 μm particle size column as a stationary phase and water: methanol (50:50, V/V pH adjusted to 3.9 with CF3COOH acid as a mobile phase, maintaining the flow rate at 1.0 mL min−1 with UV detection at 210 nm. The proposed method was successfully applied for the determination of HBB and PAR in pure form over a concentration range of 2.0–50.0 μg mL−1 for HBB with mean percentage recovery of 100.10 ± 0.475 and over a concentration range of 5.0–200.0 μg mL−1 for PAR with mean percentage recovery of 99.87 ± 0.942 and in their pharmaceutical formulations (Buscopan plus® tablets, Buscamol® tablets and Buscopan plus® suppositories.

Simultaneous Estimation of Gemcitabine Hydrochloride and Capecitabine Hydrochloride in Combined Tablet Dosage Form by RP-HPLC Method

Directory of Open Access Journals (Sweden)

V. Rajesh

2011-01-01

Full Text Available A new reverse phase high performance liquid chromatography (RP-HPLC method has been developed for the simultaneous estimation of gemcitabine hydrochloride and capecitabine hydrochloride in combined tablet dosage form. An inertsil ODS-3 C-18 column having dimensions of 250×4.6 mm and particle size of 5 µm, with mobile phase containing a mixture of acetonitrile : water : triethyelamine in the ratio of (70 : 28 : 2v/v was used. The pH of mobile phase was adjusted to 4.0 with ortho-phosphoric acid. The flow rate was 1 mL/min and the column effluents were monitored at 260 nm. The retention time for gemcitabine hydrochloride and capecitabine hydrochloride was found to be 2.76 and 2.3 min respectively. The proposed method was validated in terms of linearity, accuracy, precision, limit of detection, limit of quantitation and robustness. The method was found to be linear in the range of 10-50 µg/mL and 4-24 µg/mL for gemcitabine hydrochloride and capecitabine hydrochloride, with regression coefficient r = 0.999 and r = 0.999, respectively.

Implementation of the neutron noise technique under the UBERA-6 project

International Nuclear Information System (INIS)

Gomez, Angel; Bellino, Pablo A.

2009-01-01

Using the neutron noise technique, kinetics parameters estimations and power calibration were performed in the new core of the RA-6 reactor. These activities were carried on under the nuclear start-up of the UBERA-6 project, which consist in the change of core and power increase of the reactor. In a first stage, in joint with the power estimation, the decay constant of the prompt neutrons (α c ) was estimated. Its value was found to agree with the calculations obtained from neutron codes. Lately, in the high power stage, estimators of the calibration factors for the 16 N detection device were obtained. A thorough analysis of the linearity of the instrumentation used was done, and an alternative methodology was applied in order to estimate the aforementioned factor. The calibration factor obtained by the neutron noise technique was in agreement with the one obtained by thermal balance. (author)

Simultaneous RP-HPLC determination of sparfloxacin and dexamethasone in pharmaceutical formulations

Directory of Open Access Journals (Sweden)

Syed Naeem Razzaq

2013-06-01

Full Text Available The present study describes the development and subsequent validation of simple and accurate stability indicating RP-HPLC method for the determination of sparfloxacin and dexamethasone in pharmaceutical formulations in the presence of their stress-induced degradation products. Both the drugs and their stress-induced degradation products were separated within 10 minutes using C8 column and mixture of methanol and 0.02 M phosphate buffer pH 3.0 (60:40 v/v, respectively as mobile phase at 270 nm using diode array detector. Regression analysis showed linearity in the range of 15-105 µg/mL for sparfloxacin and 5-35 µg/mL for dexamethasone. All the analytes were adequately resolved with acceptable tailing. Peak purity of the two drugs was also greater than 0.9999, showing no co-elution peaks. The developed method was applied for simultaneous determination of sparfloxacin and dexamethasone in pharmaceutical formulations for stability studies.

Nuclear fuel cycle facilities and RP: the case of Brazil

International Nuclear Information System (INIS)

Tranjan Filho, Alfredo; Costa, Cesar Gustavo S.

2008-01-01

Full text: The renewed nuclear energy scenario, national and worldwide, calls for the strengthening of all activities involving the nuclear fuel production, from uranium extraction at the mines to fuel assemblies delivery at the nuclear power plants, which in Brazil is the mission of the Industrias Nucleares do Brasil (INB). With only a third of its territory prospected, Brazil currently has the sixth largest uranium reserve in the world. Brazil's three main deposits are: the Caldas mine (in the state of Minas Gerais) the first mineral-industrial complex that processed uranium, developed in 1982, and presently being decommissioned; Caetite mine and processing facility (located in the state of Bahia), nowadays operational and with a current production capacity of 400 tonnes per year of uranium concentrates, being in trend of doubling its annual capacity; and the Itataia/Santa Quiteria deposit (in Ceara State), the largest geological uranium reserve in Brazil, although its feasible future production depends on the exploration of the phosphate associated to it. Concerning the nuclear fuel fabrication, INB plant at Resende (in the state of Rio de Janeiro) is responsible for the conversion of Uf 6 to UO 2 the production of fuel pellets and the assembly of the fuel elements, in order to supply the demands of Brazil's two operating PWR (Angra 1 and Angra 2). In addition, in May 2006, INB-Resende inaugurated the uranium enrichment facility, employing the ultra-centrifugation technology. Today still in its first phase of operation, when completed the enrichment facility is intended to provide 100 percent of the domestic requirements, eventually by the year 2015. Detailing present status and future perspectives of INB, in face of the global and national renaissance of nuclear energy, this paper addresses the Radiation Protection (RP) aspects related to INB's achievements and performance, as well as the pressing future challenges to be dealt with, in order to guarantee

Egy fénykép ürügyén : Minni Nurme / Károly Árpás

Index Scriptorium Estoniae

Árpás, Károly

2003-01-01

Luulenäited tekstis: "Éjszaka árnyék vagyok..."; "A felhőkönnyek csÍraszálai..."; Őszi esten; "Fekete szivárványt..."; Folyosók; Szeptember; "eső esik hervasztja..."; Életöröm; "gyerekek alusznak..." (tõlkinud Károly Árpás)

50 CFR 81.6 - Project Agreement.

Science.gov (United States)

2010-10-01

...) FINANCIAL ASSISTANCE-WILDLIFE SPORT FISH RESTORATION PROGRAM CONSERVATION OF ENDANGERED AND THREATENED..., the period of time necessary to accomplish the objectives, and both the Federal and State costs. All... Office of Management and Budget Circular No. A-95 (as revised). (d) The Project Agreement will follow...

Astrophysical r- and rp-processes, and radioactive nuclear beams

International Nuclear Information System (INIS)

Boyd, Richard N.

1998-01-01

The modern description of the r-process follows naturally from α-rich freezeout, thought to occur in the hot neutrino wind just beyond the nascent neutron star in a type II supernova. Initially, all pre-existing nuclei are reduced to α-particles and neutrons. As the environment cools, nuclei up to about mass 90 to 100 u are synthesized, in nuclear statistical equilibrium, in about 1 s. In the next few seconds, the remaining neutrons are captured to form the r-process progenitors, which then decay to the r-process nuclides. The rp-process occurs in a high-temperature H-rich environment. It is one of the processes that synthesize the p-process nuclei, the most neutron-poor nuclei in the periodic table. It is thought to occur during the explosion of a C-O white dwarf in a type Ia supernova or in a binary system during accretion onto a white dwarf or a neutron star. It appears to be capable of forming the p-nuclei up to about mass 90 u. Both processes pass through nuclei that are far from stability. Thus, their description requires the masses, half-lives, decay modes, and structure of these nuclei. The next generation of radioactive beam facilities promises to allow the study of many such nuclei. (author)

Bone marrow-derived macrophages exclusively expressed caveolin-2: The role of inflammatory activators and hypoxia

Czech Academy of Sciences Publication Activity Database

Macečková, Michaela; Martíšková, Hana; Koudelka, Adolf; Kubala, Lukáš; Lojek, Antonín; Pekarová, Michaela

2015-01-01

Roč. 220, č. 11 (2015), s. 1266-1274 ISSN 0171-2985 R&D Projects: GA ČR(CZ) GP13-40882P; GA MŠk(CZ) EE2.3.30.0030 Grant - others:GAAV(CZ) M200041208 Institutional support: RVO:68081707 Keywords : NF-KAPPA-B * RAT PERITONEAL-MACROPHAGES * NITRIC-OXIDE PRODUCTION Subject RIV: EC - Immunology Impact factor: 2.781, year: 2015

Numerical solution of compressible and incompressible unsteady flows in channel inspired by vocal tract

Czech Academy of Sciences Publication Activity Database

Pořízková, P.; Kozel, Karel; Horáček, Jaromír

2014-01-01

Roč. 270, November (2014), s. 323-329 ISSN 0377-0427 R&D Projects: GA ČR(CZ) GAP101/11/0207; GA ČR(CZ) GAP101/10/1329 Institutional support: RVO:61388998 Keywords : compressible * incompressible * unsteady * CFD * acoustic * vocal tract Subject RIV: BI - Acoustics Impact factor: 1.266, year: 2014 http://www.sciencedirect.com/science/article/pii/S0377042713007188#

Philometrids (Nematoda: Philometridae) from fishes of Lake Turkana, Kenya, including two new species of Philometra and erection of Afrophilometra gen. sp

Czech Academy of Sciences Publication Activity Database

Moravec, František; Charo-Karisa, H.; Jirků, Miloslav

2009-01-01

Roč. 56, č. 1 (2009), s. 41-54 ISSN 0015-5683 R&D Projects: GA MŠk LC522; GA AV ČR KJB600960813; GA ČR(CZ) GA524/06/0170 Institutional research plan: CEZ:AV0Z60220518 Keywords : Philometridae * freshwater fishes * Kenya Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 1.266, year: 2009

Spent Nuclear Fuel (SNF) Project Cask and MCO Helium Purge System Design Review Completion Report - Project A.5 and A.6

International Nuclear Information System (INIS)

ARD, K.E.

2000-01-01

This report documents the results of the design verification performed on the Cask and Multiple Canister Over-pack (MCO) Helium Purge System. The helium purge system is part of the Spent Nuclear Fuel (SNF) Project Cask Loadout System (CLS) at 100K area. The design verification employed the ''Independent Review Method'' in accordance with Administrative Procedure (AP) EN-6-027-01

Specific in vivo binding in the rat brain of [{sup 18}F]RP 62203: A selective 5-HT{sub 2A} receptor radioligand for positron emission tomography

Energy Technology Data Exchange (ETDEWEB)

Besret, Laurent; Dauphin, Francois; Huard, Cecile; Lasne, Marie-Claire; Vivet, Richard; Mickala, Patrick; Barbelivien, Alexandra; Baron, Jean-Claude

1996-02-01

In vivo pharmacokinetic and brain binding characteristics of [{sup 18}F]RP 62203, a selective high-affinity serotonergic 5-HT{sub 2A} receptor antagonist, were assessed in the rat following intravenous injection of trace amount of the radioligand. The radioactive distribution profile observed in the brain 60 min after injection was characterized by greater than fourfold higher uptake in neocortex as compared to cerebellum (0.38 {+-} 0.07% injected dose/g, % ID/g and 0.08 {+-} 0.01 ID/g, respectively), consistent with in vivo specific binding to the 5-HT{sub 2A} receptor. Furthermore, specific [{sup 18}F]RP 62203 binding significantly correlated with the reported in vitro distribution of 5-HT{sub 2A} receptors, but not with known concentration profiles of dopaminergic D{sub 2} or adrenergic {alpha}{sub 1} receptors. Finally, detectable specific binding was abolished by pretreatment with large doses of ritanserin, a selective 5-HT{sub 2A} antagonist, which resulted in uniform uptakes across cortical, striatal and cerebellar tissues. Thus, [{sup 18}F]RP 62203 appears to be a promising selective tool to visualize and quantify 5-HT{sub 2A} brain receptors in vivo with positron emission tomography.

Agile project management with GreenHopper 6 blueprints

CERN Document Server

Malik, Jaibeer

2013-01-01

A step-by-step tutorial-based approach.This book is of great help for agile teams who are already using or planning to use the GreenHopper tooling system to execute agile projects. It suits all roles in an agile project including system administrators, stakeholders, product owners, scrum masters, and team members. Fundamental knowledge of JIRA is essential.

Boron-purity-dependent Raman spectra of CaB6

International Nuclear Information System (INIS)

Song, M.; Yang, I. S.; Kim, J. Y.; Cho, B. K.

2006-01-01

We report significant differences in the Raman spectra of two different kinds of CaB 6 single crystals grown from boron with a purity of 99.9 % (3N) or 99.9999 % (6N). Our Raman spectra of CaB 6 (3N) show peaks around 781 (T 2g ), 1141 (E g ), and 1283 cm -1 (A 1g ), and they are very similar to previous Raman spectra of CaB 6 . The E g mode shows a characteristic double-peak feature due to an additional weak broad peak centered around 1158 cm -1 . However, the Raman spectra of CaB 6 (6N) show sharp peaks around 771 (T 2g ), 1137 (E g ), and 1266 cm -1 (A 1g ). The peak frequencies are down shifted as much as ∼17 cm -1 . In addition, no additional peak feature is observed for the E g mode so that the mode is symmetric in the case of CaB 6 (6N). The X-ray powder diffraction patterns for both CaB 6 (3N) and CaB 6 (6N) show that the lattice parameters are essentially the same. The majority of the impurity in the 99.9 %-pure (3N) boron is C. Thus, we doped CaB 6 (6N) with C, making Ca(B 0.995 C 0.005 ) 6 , and looked for differences in the Raman spectra. The Raman spectra of Ca(B 0.995 C 0.005 ) 6 are nearly identical to those of CaB 6 (6N), indicating that the differences between the Raman spectra of CaB 6 (3N) and CaB 6 (6N) are not due to a C impurity. The Raman results show that the presence of impurities, not the amount of them, is enough to trigger local symmetry breaking in CaB 6 . The broadening of T 2g , the additional E g2 mode and the asymmetry of A 1g in CaB 6 (3N) can be understood in terms of the symmetry of the arrangements of the boron octahedra lowered by local symmetry breaking.

Inhibition of bovine viral diarrhea virus RNA synthesis by thiosemicarbazone derived from 5,6-dimethoxy-1-indanone.

Science.gov (United States)

Castro, Eliana F; Fabian, Lucas E; Caputto, María E; Gagey, Dolores; Finkielsztein, Liliana M; Moltrasio, Graciela Y; Moglioni, Albertina G; Campos, Rodolfo H; Cavallaro, Lucía V

2011-06-01

In the present work, we described the activity of the thiosemicarbazone derived from 5,6-dimethoxy-1-indanone (TSC), which we previously characterized as a new compound that inhibits bovine viral diarrhea virus (BVDV) infection. We showed that TSC acts at a point of time that coincides with the onset of viral RNA synthesis and that it inhibits the activity of BVDV replication complexes (RCs). Moreover, we have selected five BVDV mutants that turned out to be highly resistant to TSC but still susceptible to ribavirin (RBV). Four of these resistant mutants carried an N264D mutation in the viral RNA-dependent RNA polymerase (RdRp). The remaining mutant showed an A392E mutation within the same protein. Some of these mutants replicated slower than the wild-type (wt) virus in the absence of TSC, whereas others showed a partial reversion to the wt phenotype over several passages in the absence of the compound. The docking of TSC in the crystal structure of the BVDV RdRp revealed a close contact between the indane ring of the compound and several residues within the fingers domain of the enzyme, some hydrophobic contacts, and hydrogen bonds with the thiosemicarbazone group. Finally, in the mutated RdRp from resistant BVDV, these interactions with TSC could not be achieved. Interestingly, TSC inhibited BVDV replication in cell culture synergistically with RBV. In conclusion, TSC emerges as a new nonnucleoside inhibitor of BVDV RdRp that is synergistic with RBV, a feature that turns it into a potential compound to be evaluated against hepatitis C virus (HCV).

Development and validation of a fast RP-HPLC method for determination of methotrexate entrapment efficiency in polymeric nanocapsules.

Science.gov (United States)

Sartori, Tatiane; Seigi Murakami, Fabio; Pinheiro Cruz, Ariane; Machado de Campos, Angela

2008-07-01

A rapid and effective isocratic chromatographic procedure is successfully developed to determinate methotrexate (MTX) entrapment efficiency (EE) in polymeric nanocapsules using reversed-phase high-performance liquid chromatography. The method employed a RP-C(18) Shimadzu Shim-pack CLC-ODS (150 mm x 4.6 mm, 5 microm) column with mobile phase constituted by a mixture of water-acetonitrile-tetrahydrofuran (65:30:5 v/v/v; pH 3.0) at a flow rate of 0.8 mL/min. The eluate is monitored with a UV detector set at 313 nm. The parameters used in the validation process are: linearity, specificity, precision, accuracy, and limit of quantitation (LOQ). The linearity is evaluated by a calibration curve in the concentration range of 10-50 microg/mL and presented a correlation coefficient of 0.9998. The polymers (PLA or PLA-PEG), oil, and surfactants used in the nanocapsule formulation did not interfere with analysis and the recovery was quantitative. The intra and inter-day assay relative standard deviation were less than 0.72%. Results are satisfactory, and the method proved to be adequate for the determination of methotrexate in nanocapsules formulations.

Hydraulic analysis of emergency core cooling system of reactor RP-10; Analisis hidraulico del sistema de refrigeracion de emergencia del nucleo del reactor RP-10

Energy Technology Data Exchange (ETDEWEB)

Gallardo Padilla, Alberto; Moreyra, Geraldo Lazaro; Nieto Malpartida, Manuel [Instituto Peruano de Energia Nuclear, Lima (Peru)]. E-mail: agallardo@ipen.gob.pe; glazaro@ipen.gob.pe; mnieto@ipen.gob.pe

2002-07-01

For design of the Emergency Core Cooling System (ECCS) of reactor RP-10 from Peru is very important the hydraulic analysis of this system. In this paper, based on a basic design of the ECCS are showed the conservation equations, the parabolic movement, being deduced from them the equations to evaluate regarding the time the variables to consider in the design: level of the emergency water in the reserve tank, flow, reaches of sprinkle, etc. In this analysis is considered a quasi-stationary flow for simplify the calculation. The developed model was implemented in a computer program denominated ECCSRP10, in language Fortran 77, whose results are shown in form graph. From analysis of results we can conclude that for the system of pipe of the ECCS the appropriate diameter is of 2 in., and that the maximum flow possible to give is of 5 m{sup 3}/h for to assure a minimum time of refrigeration of 150000 seconds. Experimental tests were made in a prototype of the pipe system being demonstrated that the obtained results of the simplified calculation agree with the values registered with a global approach of 10%. (author)

Genetic variation and recombination of RdRp and HSP 70h genes of Citrus tristeza virus isolates from orange trees showing symptoms of citrus sudden death disease

Directory of Open Access Journals (Sweden)

Pappas Georgios J

2008-01-01

Full Text Available Abstract Background Citrus sudden death (CSD, a disease that rapidly kills orange trees, is an emerging threat to the Brazilian citrus industry. Although the causal agent of CSD has not been definitively determined, based on the disease's distribution and symptomatology it is suspected that the agent may be a new strain of Citrus tristeza virus (CTV. CTV genetic variation was therefore assessed in two Brazilian orange trees displaying CSD symptoms and a third with more conventional CTV symptoms. Results A total of 286 RNA-dependent-RNA polymerase (RdRp and 284 heat shock protein 70 homolog (HSP70h gene fragments were determined for CTV variants infecting the three trees. It was discovered that, despite differences in symptomatology, the trees were all apparently coinfected with similar populations of divergent CTV variants. While mixed CTV infections are common, the genetic distance between the most divergent population members observed (24.1% for RdRp and 11.0% for HSP70h was far greater than that in previously described mixed infections. Recombinants of five distinct RdRp lineages and three distinct HSP70h lineages were easily detectable but respectively accounted for only 5.9 and 11.9% of the RdRp and HSP70h gene fragments analysed and there was no evidence of an association between particular recombinant mosaics and CSD. Also, comparisons of CTV population structures indicated that the two most similar CTV populations were those of one of the trees with CSD and the tree without CSD. Conclusion We suggest that if CTV is the causal agent of CSD, it is most likely a subtle feature of population structures within mixed infections and not merely the presence (or absence of a single CTV variant within these populations that triggers the disease.

Aspects of 6-[18F]fluoro-L-DOPA preparation: precursor synthesis, preparative HPLC purification and determination of radiochemical purity

International Nuclear Information System (INIS)

Fuechtner, F.; Angelberger, P.; Kvaternik, H.; Hammerschmidt, F.; Simovc, B. Peric; Steinbach, J.

2002-01-01

A modified method for the synthesis of the intermediate product N-Boc-3,4-di(Boc-O)-6-iodo-L-phenylalanine ethyl ester of the [ 18 F]FDOPA precursor preparation was developed. With the application of bis-(trifluoroacetoxy)-iodobenzene for the iodination step with elemental iodine the yield of the intermediate can be increased from 12% to 50-60%. By replacing silica-gel-based RP HPLC column by a polymer-based column for semi-preparative purification of [ 18 F]FDOPA from the reaction mixture the radiochemical purity of the final product can be increased up to >99%. For the determination of the radiochemical impurity [ 18 F]fluoride a HPLC method using a column with polymer-based RP material was introduced

Biogeochemical protocols and diagnostics for the CMIP6 Ocean Model Intercomparison Project (OMIP

Directory of Open Access Journals (Sweden)

J. C. Orr

2017-06-01

Full Text Available The Ocean Model Intercomparison Project (OMIP focuses on the physics and biogeochemistry of the ocean component of Earth system models participating in the sixth phase of the Coupled Model Intercomparison Project (CMIP6. OMIP aims to provide standard protocols and diagnostics for ocean models, while offering a forum to promote their common assessment and improvement. It also offers to compare solutions of the same ocean models when forced with reanalysis data (OMIP simulations vs. when integrated within fully coupled Earth system models (CMIP6. Here we detail simulation protocols and diagnostics for OMIP's biogeochemical and inert chemical tracers. These passive-tracer simulations will be coupled to ocean circulation models, initialized with observational data or output from a model spin-up, and forced by repeating the 1948–2009 surface fluxes of heat, fresh water, and momentum. These so-called OMIP-BGC simulations include three inert chemical tracers (CFC-11, CFC-12, SF6 and biogeochemical tracers (e.g., dissolved inorganic carbon, carbon isotopes, alkalinity, nutrients, and oxygen. Modelers will use their preferred prognostic BGC model but should follow common guidelines for gas exchange and carbonate chemistry. Simulations include both natural and total carbon tracers. The required forced simulation (omip1 will be initialized with gridded observational climatologies. An optional forced simulation (omip1-spunup will be initialized instead with BGC fields from a long model spin-up, preferably for 2000 years or more, and forced by repeating the same 62-year meteorological forcing. That optional run will also include abiotic tracers of total dissolved inorganic carbon and radiocarbon, CTabio and 14CTabio, to assess deep-ocean ventilation and distinguish the role of physics vs. biology. These simulations will be forced by observed atmospheric histories of the three inert gases and CO2 as well as carbon isotope ratios of CO2. OMIP-BGC simulation

Biogeochemical Protocols and Diagnostics for the CMIP6 Ocean Model Intercomparison Project (OMIP)

Science.gov (United States)

Orr, James C.; Najjar, Raymond G.; Aumont, Olivier; Bopp, Laurent; Bullister, John L.; Danabasoglu, Gokhan; Doney, Scott C.; Dunne, John P.; Dutay, Jean-Claude; Graven, Heather;

Biogeochemical protocols and diagnostics for the CMIP6 Ocean Model Intercomparison Project (OMIP)

Science.gov (United States)

Orr, James C.; Najjar, Raymond G.; Aumont, Olivier; Bopp, Laurent; Bullister, John L.; Danabasoglu, Gokhan; Doney, Scott C.; Dunne, John P.; Dutay, Jean-Claude; Graven, Heather; Griffies, Stephen M.; John, Jasmin G.; Joos, Fortunat; Levin, Ingeborg; Lindsay, Keith; Matear, Richard J.; McKinley, Galen A.; Mouchet, Anne; Oschlies, Andreas; Romanou, Anastasia; Schlitzer, Reiner; Tagliabue, Alessandro; Tanhua, Toste; Yool, Andrew

2017-06-01

The Ocean Model Intercomparison Project (OMIP) focuses on the physics and biogeochemistry of the ocean component of Earth system models participating in the sixth phase of the Coupled Model Intercomparison Project (CMIP6). OMIP aims to provide standard protocols and diagnostics for ocean models, while offering a forum to promote their common assessment and improvement. It also offers to compare solutions of the same ocean models when forced with reanalysis data (OMIP simulations) vs. when integrated within fully coupled Earth system models (CMIP6). Here we detail simulation protocols and diagnostics for OMIP's biogeochemical and inert chemical tracers. These passive-tracer simulations will be coupled to ocean circulation models, initialized with observational data or output from a model spin-up, and forced by repeating the 1948-2009 surface fluxes of heat, fresh water, and momentum. These so-called OMIP-BGC simulations include three inert chemical tracers (CFC-11, CFC-12, SF6) and biogeochemical tracers (e.g., dissolved inorganic carbon, carbon isotopes, alkalinity, nutrients, and oxygen). Modelers will use their preferred prognostic BGC model but should follow common guidelines for gas exchange and carbonate chemistry. Simulations include both natural and total carbon tracers. The required forced simulation (omip1) will be initialized with gridded observational climatologies. An optional forced simulation (omip1-spunup) will be initialized instead with BGC fields from a long model spin-up, preferably for 2000 years or more, and forced by repeating the same 62-year meteorological forcing. That optional run will also include abiotic tracers of total dissolved inorganic carbon and radiocarbon, CTabio and 14CTabio, to assess deep-ocean ventilation and distinguish the role of physics vs. biology. These simulations will be forced by observed atmospheric histories of the three inert gases and CO2 as well as carbon isotope ratios of CO2. OMIP-BGC simulation protocols are

Integrity and activity of photosystem 2 complexes isolated from the thermophilic cyanobacterium Synechococcus elongatus using various detergents

Czech Academy of Sciences Publication Activity Database

Šetlíková, Eva; Sofrová, D.; Prášil, Ondřej; Budáč, Petr; Koblížek, Michal; Šetlík, Ivan

1999-01-01

Roč. 37, č. 2 (1999), s. 183-200 ISSN 0300-3604. [Regional Photosynthesis Workshop /2./. Lipno, 31.01.1999-03.02.1999] R&D Projects: GA ČR GA206/96/1266; GA ČR GA206/98/P110; GA MŠk VS96085; GA MŠk ME 138 Grant - others:NATO(EC) LG970388 Subject RIV: ED - Physiology Impact factor: 0.734, year: 1999

The LEDA Traitbase: A database of life-history traits of Northwest European flora

Czech Academy of Sciences Publication Activity Database

Kleyer, M.; Bekker, R.M.; Knevel, I.C.; Bakker, J. P.; Thompson, K.; Sonnenschein, M.; Poschold, P.; Van Groenendael, J. M.; Klimeš, Leoš; Klimešová, Jitka; Klotz, S.; Rusch, G.M.; Hermy, M.; Adriaens, D.; Boedeltje, G.; Bossuyt, B.; Dannemann, A.; Endels, P.; Götzenberger, L.; Hodgson, J.G.; Jackel, A.K.; Kühn, I.; Kunzmann, D.; Ozinga, W.A.; Römermann, C.; Stadler, M.; Schegelmilch, J.; Steendam, H.J.; Tackenberg, O.; Willman, B.; Cornelissen, J.H.C.; Eriksson, O.; Garnier, E.; Peco, B.

2008-01-01

Roč. 96, č. 6 (2008), s. 1266-1274 ISSN 0022-0477 Grant - others:EU(XE) EVR1-CT-2002-40022 Institutional research plan: CEZ:AV0Z60050516 Keywords : persistence * disperse * regeneration Subject RIV: EF - Botanics Impact factor: 4.262, year: 2008

Effective parameters and neutrography dispersion in the RP-10 reactor: Preliminary study

International Nuclear Information System (INIS)

Lopez, Alcides; Ticona, Santiago

2014-01-01

This paper presents a breakthrough in the study of the most important effective parameters, alternative to the standards, of the neutrography acquisition system, considering an irradiation point source and an effective register distance, as well as the dispersive effect that some kind of materials can produce when free neutrons impact them. It was observed that they do not only depend on the shape and distance from the object to the photographic screen or the neutron beam divergence, but also on the constitution of these materials. Hydrogenated materials as well as micro and nano structured solids are capable to produce significant neutron scattering. The study is developed through the analysis of images obtained by neutrography using the neutron beam of the RP-10 nuclear reactor. False color applications to the intensity of the neutron flux which gives a color contrast and simulation of 3D images intensities have helped to interpret the obtained information. (authors).

Synthesis Study of a 6-Element Non-Uniform Array with Tilted Elements for CLARREO Project

Science.gov (United States)

Jamnejad, Vahraz; Hoorfar, Ahmad

2012-01-01

This paper presents the results of a preliminary study of the gain/pattern properties of a 6-element Radio Occultation (RO) array for the proposed CLARREO (Climate Absolute Radiance and Refractivity Observatory (CLARREO) Project. CLARREO is one of the 4 highest priority missions recommended in the National Research Council Earth Science Decadal Survey.

[99m Tc (CNCH2COO-)6]-5: preliminary evaluation as tracer for studies of renal function

International Nuclear Information System (INIS)

Verdera, E.S.; Lopes L, J.J.; Balter, H.; Leon, E.; Oliver, P.; Tagle, R.

1992-01-01

The preliminary results for obtaining [ 99m Tc (CNCH 2 COO - )6] -5 are described. This complex is prepared by chemical hydrolysis of resultant molecule from carboethoxi methyl isonitrile labelling with Na 99m Tc O 4 and the yield of these reactions is determined by RP-HPLC. The radiopharmaceutical behaviour in dogs are made, confirming the attributes of [ 99m Tc (CNCH 2 COO - )6] 5 as radiodiagnosis for renal function studies. (C.G.C.)

Influence of the Diameter of Dental Implants Replacing Single Molars: 3- to 6-Year Follow-Up.

Science.gov (United States)

Mendonça, Jose Alfredo; Senna, Plinio Mendes; Francischone, Carlos Eduardo; Francischone Junior, Carlos Eduardo; Sotto-Maior, Bruno Salles

The aim of this study was to evaluate the influence of the implant diameter on marginal bone remodeling around dental implants replacing single molars after a follow-up period of 3 to 6 years. Patients who received dental implants with an external hexagon platform in healed sites to support a single metal-ceramic crown in the molar region were recalled to the office. The implantation sites and implant length information were recorded, and the implants were divided according to the implant diameter: regular (RP) or wide (WP). Each implant was assessed by digital periapical radiography, using a sensor holder for the paralleling technique. The marginal bone remodeling was determined as the distance from the implant platform to the first bone-to-implant contact, and the known implant length was used to calibrate the images in the computer software. The follow-up measurements were compared with those obtained from the radiograph taken at the time of prosthetic loading to determine the late bone remodeling. The independent t test was used to compare data. A total of 67 implants from 46 patients were evaluated with a mean follow-up period of 4.5 ± 1.0 years. The RP group comprised 36 implants from 29 patients (mean age: 58.3 ± 10.6 years), while 31 implants from 17 patients (mean age: 56.9 ± 11.5 years) were included in the WP group. The RP group presented lower survival rates (86.1%) than the WP group (100.0%). Similar marginal bone loss (P implants exhibited lower incidence failures, the bone levels were similar after the prosthetic loading around regular- and wide-diameter implants supporting single molar crowns.

Low Complexity Tail-Biting Trellises for Some Extremal Self-Dual Codes

OpenAIRE

Olocco , Grégory; Otmani , Ayoub

2002-01-01

International audience; We obtain low complexity tail-biting trellises for some extremal self-dual codes for various lengths and fields such as the [12,6,6] ternary Golay code and a [24,12,8] Hermitian self-dual code over GF(4). These codes are obtained from a particular family of cyclic Tanner graphs called necklace factor graphs.

Inhibition of Bovine Viral Diarrhea Virus RNA Synthesis by Thiosemicarbazone Derived from 5,6-Dimethoxy-1-Indanone▿

Science.gov (United States)

Castro, Eliana F.; Fabian, Lucas E.; Caputto, María E.; Gagey, Dolores; Finkielsztein, Liliana M.; Moltrasio, Graciela Y.; Moglioni, Albertina G.; Campos, Rodolfo H.; Cavallaro, Lucía V.

2011-01-01

In the present work, we described the activity of the thiosemicarbazone derived from 5,6-dimethoxy-1-indanone (TSC), which we previously characterized as a new compound that inhibits bovine viral diarrhea virus (BVDV) infection. We showed that TSC acts at a point of time that coincides with the onset of viral RNA synthesis and that it inhibits the activity of BVDV replication complexes (RCs). Moreover, we have selected five BVDV mutants that turned out to be highly resistant to TSC but still susceptible to ribavirin (RBV). Four of these resistant mutants carried an N264D mutation in the viral RNA-dependent RNA polymerase (RdRp). The remaining mutant showed an A392E mutation within the same protein. Some of these mutants replicated slower than the wild-type (wt) virus in the absence of TSC, whereas others showed a partial reversion to the wt phenotype over several passages in the absence of the compound. The docking of TSC in the crystal structure of the BVDV RdRp revealed a close contact between the indane ring of the compound and several residues within the fingers domain of the enzyme, some hydrophobic contacts, and hydrogen bonds with the thiosemicarbazone group. Finally, in the mutated RdRp from resistant BVDV, these interactions with TSC could not be achieved. Interestingly, TSC inhibited BVDV replication in cell culture synergistically with RBV. In conclusion, TSC emerges as a new nonnucleoside inhibitor of BVDV RdRp that is synergistic with RBV, a feature that turns it into a potential compound to be evaluated against hepatitis C virus (HCV). PMID:21430053

Emergency automatic commutation of the ventilation system of the RP-10 nuclear reactor; Conmutacion automatica de emergencia del sistema de ventilacion del reactor nuclear RP-10

Energy Technology Data Exchange (ETDEWEB)

Castillo, Walter; Corimanya, Mario; Ovalle, Edgar; Anaya, Olgger; Veramendi, Emilio [Direccion de Produccion, Instituto Peruano de Energia Nuclear, Av. Jose Saco km 12.5, Carabayllo, Lima (Peru)

2013-07-01

The present paper summarizes the achievements in the design and implementation of a system for monitoring and automatic control of radioactive effluents from the chimney of the RP-10 reactor, using as hardware an Arduino UNO platform containing an ATMEGA 328 programmable micro controller to which has been added LCD screen to display the values, a keyboard and an EEPROM memory data, where the limit of the level of radiation is fixed. The radiation level in the air of the reactor hall, going up the chimney is counted by a radiation monitor called MAB1000, and data are supplied to the new system. When the radiation level is above the national and international standards, the new design makes work a relay, so that the ventilation system is automatically switched to operate in emergency condition, preventing the release of radioactive contaminants into the environment. After installing the new design, it was verified that removed by the radiation monitor MAB1000, value is identical to that shown in the new system. Additionally, the operation of the relay was tested successfully with radioactive sources to switch the ventilation system to the emergency condition. (authors).

Gradient HPLC of antibiotics in urine, ground water, chicken muscle, hospital wastewater, and pharmaceutical samples using C-18 and RP-amide columns.

Science.gov (United States)

Kumar, Ashwini; Kumar Malik, Ashok; Kumar Tewary, Dhananjay; Singh, Baldev

2008-02-01

A simple and highly sensitive high pressure liquid chromatographic (HPLC-UV) method has been developed for the determination of ofloxacin, lomefloxacin, cinoxacin, and nalidixic acid, in mobile phase citrate buffer (0.001 M) of pH 4.5 prepared in water (X), methanol (Y), and ACN (Z) using gradient at a flow rate of 1.0 mL/min by direct UV absorbance detection at lambda = 280 nm. Separation of analytes was studied on the C-18 and RP-amide columns and best results were observed on the RP-amide column with LODs (3.3 x S/m) 0.89, 0.55, 0.67, and 1.41 ng/mL for ofloxacin, lomefloxacin, cinoxacin, and nalidixic acid, respectively, and better RSD than the C-18 column. The recovery of Fluoroquinolones (FQs) in urine, ground water, hospital wastewater, and chicken muscle using this method is more than 90%. The method was successfully applied to the analysis of ofloxacin, lomefloxacin, cinoxacin, and nalidixic acid in urine, ground water, pharmaceutical dosage forms, hospital wastewater, and chicken muscle.

Analytic continuation and perturbative expansions in QCD

Czech Academy of Sciences Publication Activity Database

Caprini, I.; Fischer, Jan

2002-01-01

Roč. 24, - (2002), s. 127-135 ISSN 1434-6044 R&D Projects: GA MPO RP-4210/69 Institutional research plan: CEZ:AV0Z1010920 Keywords : perturbative expansion * quantum chromodynamics * infrared ambiguity * essential singularities Subject RIV: BF - Elementary Particles and High Energy Physics Impact factor: 6.162, year: 2002

SOFTWARE SUPPORT FOR RICH PICTURES

DEFF Research Database (Denmark)

Valente, Andrea; Marchetti, Emanuela

2010-01-01

Rich pictures (RP) are common in object-oriented analysis and design courses, but students seem to have problems in integrating them in their projects' workflow. A new software tool is being developed, specific for RP authoring. To better understand students' issues and working practice with RP...

The Carbon Dioxide Removal Model Intercomparison Project (CDRMIP): rationale and experimental protocol for CMIP6

Science.gov (United States)

Keller, David P.; Lenton, Andrew; Scott, Vivian; Vaughan, Naomi E.; Bauer, Nico; Ji, Duoying; Jones, Chris D.; Kravitz, Ben; Muri, Helene; Zickfeld, Kirsten

2018-03-01

The recent IPCC reports state that continued anthropogenic greenhouse gas emissions are changing the climate, threatening severe, pervasive and irreversible impacts. Slow progress in emissions reduction to mitigate climate change is resulting in increased attention to what is called geoengineering, climate engineering, or climate intervention - deliberate interventions to counter climate change that seek to either modify the Earth's radiation budget or remove greenhouse gases such as CO2 from the atmosphere. When focused on CO2, the latter of these categories is called carbon dioxide removal (CDR). Future emission scenarios that stay well below 2 °C, and all emission scenarios that do not exceed 1.5 °C warming by the year 2100, require some form of CDR. At present, there is little consensus on the climate impacts and atmospheric CO2 reduction efficacy of the different types of proposed CDR. To address this need, the Carbon Dioxide Removal Model Intercomparison Project (or CDRMIP) was initiated. This project brings together models of the Earth system in a common framework to explore the potential, impacts, and challenges of CDR. Here, we describe the first set of CDRMIP experiments, which are formally part of the 6th Coupled Model Intercomparison Project (CMIP6). These experiments are designed to address questions concerning CDR-induced climate reversibility, the response of the Earth system to direct atmospheric CO2 removal (direct air capture and storage), and the CDR potential and impacts of afforestation and reforestation, as well as ocean alkalinization.>

Determination of the minimum number of fuel elements of the RP-10 research reactor

International Nuclear Information System (INIS)

Languasco, J.

1989-01-01

The peruvian research reactor RP-10 is composed of a compound nucleus of boxes containing fuel plates which are cooled with light water in order to remove heat produced by fission of uranium atoms. However from a certainty viewpoint, it exists certain restrictions to design the cooling system. The most admissible caloric flux of 90.3 watts/cm 2 is deflux of 90.3 watts/cm 2 is determined on the basis of these thermic restrictions when cooling speed is 409 cm/sec permitting at least 24 fuel elements(boxes) within the nucleus. On the basis of restrictions of load loss in the nucleus, it would be permitted at least 18 fuel elements, but this quantity breaks thermic restrictions for this reason, 24 boxes in the nucleus will be the minimum number of elements

UMTRA Project Office quality assurance program plan. Revision 6

International Nuclear Information System (INIS)

1994-09-01

The Uranium Mill Tailings Remedial Action (UMTRA) Project was established to accomplish remedial actions at inactive uranium mill tailings sites. The UMTRA Project's mission is to stabilize and control the residual radioactive materials at designated sites in a safe and environmentally sound manner so as to minimize or eliminate radiation health hazards to the public. Because these efforts may involve possible risks to public health and safety, a quality assurance (QA) program that conforms to the applicable criteria has been established to control the quality of the work. This document, the Quality Assurance Program Plan (QAPP), brings into one document the essential criteria to be applied on a selective basis, depending upon the nature of the activity being conducted, and describes how those criteria shall be applied to the UMTRA Project. QA requirements contained in this QAPP shall apply to all personnel, processes, and activities, including planning, scheduling, and cost control, performed by the UMTRA Project Office and its contractors

Temporal and tissue specific regulation of RP-associated splicing factor genes PRPF3, PRPF31 and PRPC8--implications in the pathogenesis of RP.

Directory of Open Access Journals (Sweden)

Huibi Cao

2011-01-01

Full Text Available Genetic mutations in several ubiquitously expressed RNA splicing genes such as PRPF3, PRP31 and PRPC8, have been found to cause retina-specific diseases in humans. To understand this intriguing phenomenon, most studies have been focused on testing two major hypotheses. One hypothesis assumes that these mutations interrupt retina-specific interactions that are important for RNA splicing, implying that there are specific components in the retina interacting with these splicing factors. The second hypothesis suggests that these mutations have only a mild effect on the protein function and thus affect only the metabolically highly active cells such as retinal photoreceptors.We examined the second hypothesis using the PRPF3 gene as an example. We analyzed the spatial and temporal expression of the PRPF3 gene in mice and found that it is highly expressed in retinal cells relative to other tissues and its expression is developmentally regulated. In addition, we also found that PRP31 and PRPC8 as well as snRNAs are highly expressed in retinal cells.Our data suggest that the retina requires a relatively high level of RNA splicing activity for optimal tissue-specific physiological function. Because the RP18 mutation has neither a debilitating nor acute effect on protein function, we suggest that retinal degeneration is the accumulative effect of decades of suboptimal RNA splicing due to the mildly impaired protein.

Observation of a large-scale anisotropy in the arrival directions of cosmic rays above 8 x 10.sup.18./sup. eV

Czech Academy of Sciences Publication Activity Database

Aab, A.; Abreu, P.; Aglietta, M.; Blažek, Jiří; Boháčová, Martina; Chudoba, Jiří; Ebr, Jan; Juryšek, Jakub; Mandát, Dušan; Palatka, Miroslav; Pech, Miroslav; Prouza, Michael; Řídký, Jan; Schovánek, Petr; Trávníček, Petr; Vícha, Jakub

2017-01-01

Roč. 357, č. 6357 (2017), s. 1266-1270 ISSN 0036-8075 R&D Projects: GA MŠk LM2015038; GA MŠk LG15014; GA ČR(CZ) GA14-17501S Institutional support: RVO:68378271 Keywords : cosmic rays * Pierre Auger Observatory * ultrahigh energy * large-scale anisotropy Subject RIV: BF - Elementary Particles and High Energy Physics OBOR OECD: Particles and field physics Impact factor: 37.205, year: 2016

Hydraulic analysis of emergency core cooling system of reactor RP-10

International Nuclear Information System (INIS)

Gallardo Padilla, Alberto; Moreyra, Geraldo Lazaro; Nieto Malpartida, Manuel

2002-01-01

For design of the Emergency Core Cooling System (ECCS) of reactor RP-10 from Peru is very important the hydraulic analysis of this system. In this paper, based on a basic design of the ECCS are showed the conservation equations, the parabolic movement, being deduced from them the equations to evaluate regarding the time the variables to consider in the design: level of the emergency water in the reserve tank, flow, reaches of sprinkle, etc. In this analysis is considered a quasi-stationary flow for simplify the calculation. The developed model was implemented in a computer program denominated ECCSRP10, in language Fortran 77, whose results are shown in form graph. From analysis of results we can conclude that for the system of pipe of the ECCS the appropriate diameter is of 2 , and that the maximum flow possible to give is of 5 m 3 /h for to assure a minimum time of refrigeration of 150000 seconds. Experimental tests were made in a prototype of the pipe system being demonstrated that the obtained results of the simplified calculation agree with the values registered with a global approach of 10%. (author)

Stability indicating RP-HPLC method for simultaneous determination of pantoprazole sodium and itopride hydrochloride in bulk and capsule

Directory of Open Access Journals (Sweden)

Krishna R. Gupta

2011-03-01

Full Text Available A stability indicating reversed-phase HPLC method has been developed and subsequently validated for simultaneous estimation of pantoprazole present as pantoprazole sodium sesquihydrate (PSS, and itopride hydrochloride from their combination product. The proposed RP-HPLC method utilizes a Phenomenex® C18, 5 µm, 250 mm X 4.6 mm i.d. column, mobile phase consisting of phosphate buffer and acetonitrile in the proportion of 55:45 (v/v with apparent pH adjusted to 5.0, and UV detection at 289.0 nm using a UV detector. PAN, ITH and their combination drug product were exposed to thermal, photolytic, hydrolytic and oxidative stress conditions, and the stressed samples were analyzed by the proposed method. The described method was linear over a range of 4-20 µg/mL for PAN and 15-75 µg/mL for ITH. The mean recoveries were 100.02 and 99.88 for PAN and ITH, respectively. Chromatographic peak purity data of PAN and ITH indicated no co-eluting peaks with the main peaks of drugs which demonstrated the specificity of assay method for their estimation in presence of degradation products. The proposed method can be useful in the quality control of combination drug products.

Demands on project management of comprehensive modernization projects in the electrical systems area. Example of modernization of electrical systems of Kozloduy NPP Unit 5 and 6

International Nuclear Information System (INIS)

Stinshoff, Helmut; Weber, Patrick

2006-01-01

In nuclear power plants, station supply with electric energy must be guaranteed any time. This applies in particular also during the implementation of complex electrical systems modernization projects. Highest demands on the project management, extensive experience and system knowledge are required. In the frame of the Modernization Program for the nuclear power plant Kozloduy unit 5 and 6 in Bulgaria Framatome ANP has approved its ability to implement a large scope of modernization measures during the refueling outages of the years 2003 to 2005. The Contract of the Modernization Program for the European Consortium Kozloduy (Framatome ANP, Atomenergoexport) was signed in July 1999 and became effective in June 2001. The project will be finished by May 2006, with the approval of the Updated Final Safety Analysis Report. The scope of hardware work has been implemented within 6 plant outages during the years 2002 to 2005. The focus of the Modernization Program is mainly oriented to nuclear safety aspects, with the aim of upgrading of the Units to a high safety level in compliance with international practice. A further section of the project is dedicated to upgrading of operational equipment. Framatome ANP personnel have shown that besides the technical challenges which had to be faced, also the intercultural and language barriers were successfully overcome. The good teamwork between the partners of the Consortium ECK, its Bulgarian subcontractors and with Kozloduy plant personnel has been an important success factor. (authors)

A genome-wide siRNA screen in mammalian cells for regulators of S6 phosphorylation.

Directory of Open Access Journals (Sweden)

Angela Papageorgiou

Full Text Available mTOR complex1, the major regulator of mRNA translation in all eukaryotic cells, is strongly activated in most cancers. We performed a genome-wide RNAi screen in a human cancer cell line, seeking genes that regulate S6 phosphorylation, readout of mTORC1 activity. Applying a stringent selection, we retrieved nearly 600 genes wherein at least two RNAis gave significant reduction in S6-P. This cohort contains known regulators of mTOR complex 1 and is significantly enriched in genes whose depletion affects the proliferation/viability of the large set of cancer cell lines in the Achilles database in a manner paralleling that caused by mTOR depletion. We next examined the effect of RNAi pools directed at 534 of these gene products on S6-P in TSC1 null mouse embryo fibroblasts. 76 RNAis reduced S6 phosphorylation significantly in 2 or 3 replicates. Surprisingly, among this cohort of genes the only elements previously associated with the maintenance of mTORC1 activity are two subunits of the vacuolar ATPase and the CUL4 subunit DDB1. RNAi against a second set of 84 targets reduced S6-P in only one of three replicates. However, an indication that this group also bears attention is the presence of rpS6KB1 itself, Rac1 and MAP4K3, a protein kinase that supports amino acid signaling to rpS6KB1. The finding that S6 phosphorylation requires a previously unidentified, functionally diverse cohort of genes that participate in fundamental cellular processes such as mRNA translation, RNA processing, DNA repair and metabolism suggests the operation of feedback pathways in the regulation of mTORC1 operating through novel mechanisms.

EC6 for Darlington - a managed-enhancements replication project

International Nuclear Information System (INIS)

Soulard, M.R.

2011-01-01

CANDU 6 design has a well-deserved reputation for lifetime performance and on-time, on-schedule construction. Candu Energy is finalizing development of an upgraded reactor product, the Enhanced CANDU 6 (EC6), which incorporates the CANDU 6's well-proven features, and adds enhancements that make the reactor even more safe and easier to operate. The EC6 is the only mid-sized reactor (700 MWe class) with a proven pedigree that meets modern reactor expectations and regulatory standards. It is sized for smaller grids and also has outstanding fuel-cycle capability. Some of the key features incorporated into the EC6 include increasing the plant's power output, shortening the overall project schedule, addressing obsolescence issues, optimizing maintenance outages and incorporating lessons learnt through feedback obtained from the operating plants. The EC6 utilizes modern computers and a distributed control system housed in an advanced control room which, along with automated testing and on-line diagnostics, make the plant easier and safer to operate, with minimal operator intervention. An array of health monitoring equipment are also installed to foretell impending equipment problems, which can be acted upon, avoiding complications that could result in forced shutdowns. Improvements to the fire protection system and enhanced security features will further protect the assets. Containment and seismic capability are upgraded to meet modern standards. The first deployment of the EC6 is anticipated in Canada; other markets are also being pursued. The EC6 reactor is designed for a target lifetime capacity factor of 92% including 94% year-to-year and a 1% Forced Loss Rate. Since the number and duration of maintenance outages impact plant capacity factors, periodic short duration maintenance outages of less than a month once every 36 months is a key target of the EC6 reactor. This objective is achieved by automating a number of tasks such as shutdown systems testing. The majority

Protective effect of sulforaphane against retinal degeneration in the Pde6rd10 mouse model of retinitis pigmentosa.

Science.gov (United States)

Kang, Kai; Yu, Minzhong

2017-12-01

Retinitis pigmentosa (RP) is a group of inherited diseases characterized by the death of rod photoreceptors, followed by the death of cone photoreceptors, progressively leading to partial or complete blindness. Currently no specific treatment is available for RP patients. Sulforaphane (SFN) has been confirmed to be an effective antioxidant in the treatment of many diseases. In this study, we tested the therapeutic effects of SFN against photoreceptor degeneration in Pde6b rd10 mice. rd10 mice and C57/BL6 wild-type (WT) mice were treated with SFN and saline, respectively, from P6 to P20. Electroretinography (ERG), terminal deoxynucleotidyl transferase dUTP nick end labeling and western blot were tested, respectively, at P21 for the analysis of retinal function, retinal cell apoptosis or death and the protein express of GRP78/BiP (TUNEL) as a marker of endoplasmic reticulum (ER) stress. Compared with the saline group, the SFN-treated group showed significantly higher ERG a-wave and b-wave amplitudes, less photoreceptor death, and the downregulation of GRP78/BiP. Our data showed that SFN ameliorated the retinal degeneration of rd10 mice, which is possibly related to the downregulation of GRP78 expression.

Positron two-dimensional angular-correlation-of-annihilation-radiation study of untwinned YBa2Cu3O6.9 in the a-axis projection

International Nuclear Information System (INIS)

Smedskjaer, L.C.; Bansil, A.; Welp, U.; Fang, Y.; Bailey, K.G.

1992-01-01

We present a two-dimensional (2D)-ACAR positron annihilation study of an untwinned YBa 2 Cu 3 O 6.9 metallic sample in the a-axis projection; all existing data from untwinned samples have employed the c projection. The data show a clear presence of the Cu-O chain related ridge Fermi surface extending along the ΓZ direction and two associated umklapp images at higher momenta. These results are consistent with the previously investigated c projection of the momentum density in YBa 2 Cu 3 O 6.9 and do not appear to indicate a strong localization of these electron states along the c direction. We compare and contrast the structures in 2D-ACAR's in the a and c projections, delineating similarities and differences between the results for the two projections

Project summary, 116-B-6-1 crib ISV [in situ vitrification] demonstration project

International Nuclear Information System (INIS)

Koegler, S.S.

1989-01-01

The 116-B Crib Demonstration Project is intended to demonstrate the emerging in situ vitrification (ISV) technology to immobilize or destroy hazardous and radioactive chemicals at an actual site. In situ vitrification is the conversion of contaminated soil into a durable glass and crystalline product through joule heating. The 116-B crib site was chosen for the demonstration because it contains both radioactive and hazardous chemicals (e.g., chromium) and presents a potential threat to environment. The project will involve sampling and analysis of the soil beneath the crib, a small-scale ISV test to verify operating parameters, vitrification of the crib, and analysis of the vitrified soil. 5 figs

The Carbon Dioxide Removal Model Intercomparison Project (CDRMIP: rationale and experimental protocol for CMIP6

Directory of Open Access Journals (Sweden)

D. P. Keller

2018-03-01

Full Text Available The recent IPCC reports state that continued anthropogenic greenhouse gas emissions are changing the climate, threatening severe, pervasive and irreversible impacts. Slow progress in emissions reduction to mitigate climate change is resulting in increased attention to what is called geoengineering, climate engineering, or climate intervention – deliberate interventions to counter climate change that seek to either modify the Earth's radiation budget or remove greenhouse gases such as CO2 from the atmosphere. When focused on CO2, the latter of these categories is called carbon dioxide removal (CDR. Future emission scenarios that stay well below 2 °C, and all emission scenarios that do not exceed 1.5 °C warming by the year 2100, require some form of CDR. At present, there is little consensus on the climate impacts and atmospheric CO2 reduction efficacy of the different types of proposed CDR. To address this need, the Carbon Dioxide Removal Model Intercomparison Project (or CDRMIP was initiated. This project brings together models of the Earth system in a common framework to explore the potential, impacts, and challenges of CDR. Here, we describe the first set of CDRMIP experiments, which are formally part of the 6th Coupled Model Intercomparison Project (CMIP6. These experiments are designed to address questions concerning CDR-induced climate reversibility, the response of the Earth system to direct atmospheric CO2 removal (direct air capture and storage, and the CDR potential and impacts of afforestation and reforestation, as well as ocean alkalinization.>

Optical imaging of oxidative stress in retinitis pigmentosa (RP) in rodent model

Science.gov (United States)

Ghanian, Zahra; Maleki, Sepideh; Gopalakrishnan, Sandeep; Sepehr, Reyhaneh; Eells, Janis T.; Ranji, Mahsa

2013-02-01

Oxidative stress (OS), which increases during retinal degenerative disorders, contributes to photoreceptor cell loss. The objective of this study was to investigate the changes in the metabolic state of the eye tissue in rodent models of retinitis pigmentosa by using the cryofluorescence imaging technique. The mitochondrial metabolic coenzymes NADH and FADH2 are autofluorescent and can be monitored without exogenous labels using optical techniques. The NADH redox ratio (RR), which is the ratio of the fluorescence intensity of these fluorophores (NADH/FAD), was used as a quantitative diagnostic marker. The NADH RR was examined in an established rodent model of retinitis pigmentosa (RP), the P23H rat, and compared to that of control Sprague-Dawley (SD) rats and P23H NIR treated rats. Our results demonstrated 24% decrease in the mean NADH RR of the eyes from P23H transgenic rats compared to normal rats and 20% increase in the mean NADH RR of the eyes from the P23H NIR treated rats compared to P23H non-treated rats.

Proceedings of the 10. national radiation protection congress

International Nuclear Information System (INIS)

Cousins, Claire; Ducou Le Pointe, Hubert; Lochard, Jacques; Vaillant, L.; Masse, Roland; Stricker, Laurent; Beaugelin-Seiller, K.; Garnier-Laplace, J.; Bernier, M.O.; Biau, A.; Bordy, J.M.; Laurier, D.; Guipaud, Olivier; Leuraud, Klervi; Rage, Estelle; Villeneuve, Sara; Clero, Enora; Samson, E.; Scanff, P.; Rannou, A.; Caldeira Ideias, P.; Paradis, H.; Boussetta, B.; Boyer, C.; Gontier, G.; Hemidy, P.Y.; Carreau Gaschereau, E.; Hartemann, P.; Menechal, P.; Mougniot, S.; Le Coz, E.; Le Goff, Pierre; Abela, G.; Chirent, T.; De Vita, A.; Drouet, F.; Hillaireau, B.; Marcillet, C.; Michoux, X.; Yadani, Fatima; Blanc, Pauline; Canal, E.; Perier, A.; Lahaye, T.; Andresz, Sylvain; Barbey, P.; Gagna, Gerald; Guetat, Ph.; Schneider, Thierry; Vaillant, Ludovic; Cherin, Herve; Roy, Catherine; Desbiolles, Alice; Roudier, Candice; Goria, Sarah; Stempfelet, Morgane; Monnereau, Alain; Lefranc, Agnes; Vacquier, Blandine; Baysson, H.; Etard, C.; Maurice, Jean-Baptiste; Milliat, Fabien; Moan, Gwennael; Petitguillaume, Alice; Roch, Patrice; Marie, Laurent; Dufay, Emilie; Magne, Isabelle; Mathieu, Peggy; Perrin, Anne; Veyret, Bernard; Clauss, Nicolas; Dabli, D.; Guillot, Sebastien; Kamoun, Hager; Mackowiak, Julien; Bensimon, Julie; Bez, Jeremy; Petitfrere, Michael

2015-06-01

The French Society of Radiation Protection (SFRP) celebrated its 50 anniversary at the occasion of the 10. national radiation protection (RP) congress. This document brings together the abstracts of the different talks given at the congress. A - Invited talks: A1 - Forward through the rearview mirror: Reflections on ICRP Past and Future (C. COUSINS); A3 - Origin and evolution of the intervening parties involvement approach in RP (J. LOCHARD); A4 - Knowledge influence and biological uncertainties on RP evolution (R. MASSE); A5 - RP history in nuclear industry: EDF an anticipation example (L. STRICKER); B - Tutorial presentations: B1 - Environment RP: towards its explicit integration in French law? (K. BEAUGELIN-SEILLER, J. GARNIER-LAPLACE); B2 - Scanner exposure in child and radio-induced risk: recent epidemiological results (M.O. BERNIER); B3 - Ionizing radiations professional exposure dose measurement: goals and evolutions (A. BIAU, J.M. BORDY); B4 - Workers RP at dismantling sites (B. BOUSSETTA, L. VAILLANT); B5 - Environment RP (part 2): EDF's methodology and experience feedback (C. BOYER, G. GONTIER, P.Y. HEMIDY); B6 - Revision of SCENIHR's 2009 opinion about the potential health impact of electromagnetic fields, from the 2011 European inaugural meeting to its 2015 publication (P. HARTEMANN); B8 - Advances in low dose epidemiological knowledge (D. LAURIER); B9 - Use of ionizing radiations at the operating theatre: what RP issues? (P. MENECHAL, S. MOUGNIOT); Session 1: RP regulations and standards, RP and society (6 presentations); Session 2: effects of Ionizing radiations on men and ecosystems (6 presentations); Session 3: patients RP (7 presentations); Session 4: Populations and ecosystems RP (4 presentations); Session 5: Occupational RP - Industry (8 presentations); Session 6: non-ionizing radiations (5 presentations); Session 7: Occupational RP - Medical (6 presentations); Session 8: Advances in dosimetry and metrology (4 presentations)

The decision-making process in dealing with populations living in areas contaminated by the Chernobyl accident. The ETHOS project

International Nuclear Information System (INIS)

Heriard-Dubreuil, G.; Schneider, T.

1998-01-01

Experience from the Chernobyl accident revealed strong disturbance in social life and stress phenomenon in the population living in the contaminated territories. The ETHOS project (founded by the radiation protection research programme of the European Commission-DG XII) has initiated an alternative approach of the rehabilitation of living conditions in the contaminated territories of the CIS in the post-accident context of Chernobyl. This project started at the beginning of 1996 and is implemented in the Republic of Belarus. Its main goal is to create the conditions for the inhabitants of contaminated territories to reconstruct their global quality of life. The main features of the methodological approach of the ETHOS project in the village of Olmany in the district of Stolyn (Brest region) since March 1996 are presented, and its implementation and first results are discussed. (R.P.)

Applications of API RP 1102 in stress analysis of buried pipelines at automated cultivation land; Avaliacao de tensoes em dutos enterrados, em area de cultura mecanizada, a luz da norma API RP 1102

Energy Technology Data Exchange (ETDEWEB)

Spagnolo, Rodrigo Alves; Castro, Newton Camelo de [PETROBRAS Transportes S.A. (TRANSPETRO), Rio de Janeiro, RJ (Brazil)

2005-07-01

According to the development of the agriculture business in the Midwest of Brazil, it can be noted that the cultivated land has imposed to the right of way pipeline weight and quantity increasing of vehicles moving above TRANSPETRO pipelines. This paper has the objective promoting discussions about this theme, presenting preceding conclusions when analyzing pipeline stresses, when the pipeline equipment are submitted to great loadings of agricultural machines. In order to execute this analysis, analytical formulations were used to compute static and dynamic loadings. These evaluations were referenced from API RP 1102 american norm. Many loading simulations, with different operating and environment configurations to the buried installations, were realized. These data were applied having the purpose of diagnosing typical situations existing in automated cultivated land crossings, related to OSBRA (Oleoduto Sao Paulo - Brasilia) right of way pipeline. (author)

Validated RP-HPLC/DAD Method for the Quantification of Insect Repellent Ethyl 2-Aminobenzoate in Membrane-Moderated Matrix Type Monolithic Polymeric Device.

Science.gov (United States)

Islam, Johirul; Zaman, Kamaruz; Chakrabarti, Srijita; Sharma Bora, Nilutpal; Mandal, Santa; Pratim Pathak, Manash; Srinivas Raju, Pakalapati; Chattopadhyay, Pronobesh

2017-07-01

A simple, accurate and sensitive reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been developed for the estimation of ethyl 2-aminobenzoate (EAB) in a matrix type monolithic polymeric device and validated as per the International Conference on Harmonization guidelines. The analysis was performed isocratically on a ZORBAX Eclipse plus C18 analytical column (250 × 4.4 mm, 5 μm) and a diode array detector (DAD) using acetonitrile and water (75:25 v/v) as the mobile phase by keeping the flow-rate constant at 1.0 mL/min. Determination of EAB was not interfered in the presence of excipients. Inter- and intra-day relative standard deviations were not higher than 2%. Mean recovery was between 98.7 and 101.3%. Calibration curve was linear in the concentration range of 0.5-10 µg/mL. Limits of detection and quantification were 0.19 and 0.60 µg/mL, respectively. Thus, the present report put forward a novel method for the estimation of EAB, an emerging insect repellent, by using RP-HPLC technique. © The Author 2017. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

78 FR 23760 - Combined Notice of Filings #1

Science.gov (United States)

2013-04-22

...-1266-000. Applicants: CalEnergy, LLC. Description: CalEnergy FERC MBR Tariff Application to be.... Docket Numbers: ER13-1267-000. Applicants: CE Leathers Company. Description: CE Leathers FERC MBR Tariff... Company MBR Tariff Application to be effective 6/3/2013. Filed Date: 4/12/13. Accession Number: 20130412...

Determination of vegetable tannins in tannery wastewater using RP-HPLC-technique with UV- and EC-detection; Bestimmung vegetabiler Gerbstoffe in Gerbereiabwasser unter Einsatz der RP-HPLC-Technik mit UV- und EC-Detektion

Energy Technology Data Exchange (ETDEWEB)

Zywicki, B.; Reemtsma, T.; Jekel, M. [Technische Univ. Berlin (Germany). Fachgebiet Wasserreinhaltung

2001-07-01

Numerous types of inorganic and organic environmental pollutants are encountered in leather tannery effluents. For reduction of the environmental impact of leather tanneries the substitution of chromium tanning agents by natural organic tanning agents (vegetable tannins) is discussed. The use of vegetable tannins leads to an elevated dissolved organic carbon content (DOC) in tannery effluent, even after biological treatment. Vegetable tannins may also be used as retanning agents, but they may, then interfere with precipitation of chromium. Therefore, an analytical method had to be established to estimate the total amount of vegetable tanning agents in the complex matrix of tannery wastewater. The analytical strategy for the determination of vegetable tannins in tannery effluents is to degrade the polyphenolic compounds by acid hydrolysis and to detect the resulting specific monomeric subunit gallic acid by RP-HPLC-technique with UV- and electrochemical detection (EC-detection). (orig.)

The COMURHEX 2 project. Investing in UF6 long-term security of supply

International Nuclear Information System (INIS)

Bouzon, Pierre; Lacombe, Philippe; Durante, Pierre; Teyssier, Patrick

2008-01-01

The front-end nuclear fuel supply chain for LWRs encompasses four major industrial stages that are mining and concentration, conversion, enrichment, and eventually fuel fabrication. The different stages involve uranium in different chemical and physical forms. Enrichment of the 235-U fissile isotope requires gaseous UF6. As the standard output of mine is U3O8, referred to as ''yellow cake'', a purely chemical stage is therefore needed to fluorinate U3O8 and turn it into UFe: this is the conversion stage. U3O8 inventories management is thus performed at the conversion sites.Purification of the mining concentrates is also needed prior to actual conversion into UFe. This step is important because the front-end supply chain facilities have strict specifications concerning impurities. The conversion stage may involve intermediary products, namely UO3 and/or UF4, depending on the industrial scheme implemented. With the Comurhex 2 project, AREVA is not only shaping the future of conversion market and contributing to the security of supply of its customers, but it is also developing innovative techniques and reorganizing the conversion process steps. Providing such guaranteed and valuable conversion supply with a brand new plant is our strong commitment to a sustainable nuclear fuel cycle. And AREVA is the first that has launched such a project, looking further ahead. The three main axes of sustainable development, economical, social, and environmental, are truly taken into account in the development of the new project.

Plutonium and minor actinides management in thermal high - temperature reactors - the EU FP6 project puma

International Nuclear Information System (INIS)

Kuijper, J. C.

2007-01-01

The High Temperature gas-cooled Reactor (HTR) can fulfil a very useful niche for the purposes of Pu and Minor Actinide (MA) incineration due to its unique and unsurpassed safety features, as well as to the attractive incentives offered by the nature of the coated particle (CP) fuel. No European reactor of this type is currently available, but there has been, and still is, considerable interest internationally. Decisions to construct such a reactor in China and in South Africa have already been made or are about to be made. Apart from the unique and unsurpassed safety features offered by this reactor type, the nature of the CP fuel offers a number of attractive characteristics. In particular, it can withstand burn-ups far beyond that in either LWR or FR systems. Demonstrations as high as 75% FIMA have been achieved. The coated particle itself offers significantly improved proliferation resistance, and finally with a correct choice of the kernel composition, it can be a very effective support for direct geological disposal of the fuel. The overall objective of the PUMA project, a Specific Targeted Research Project (STREP) within the European Union 6th Framework (EU FP6), is to investigate the possibilities for the utilisation and transmutation of plutonium and especially minor actinides in contemporary and future (high temperature) gas-cooled reactor designs, which are promising tools for improving the sustainability of the nuclear fuel cycle. This contributes to the reduction of Pu and MA stockpiles, and also to the development of safe and sustainable reactors for CO 2 -free energy generation. A number of important issues concerning the use of Pu and MA in gas-cooled reactors have already been dealt with in other projects, or are being treated in ongoing projects, e.g. as part of EU FP6. However, further steps are required to demonstrate the potential of HTRs as Pu/MA transmuters based on realistic/feasible designs of CP Pu/MA fuel and the PUMA focuses on necessary

Axure RP7 prototyping essentials

CERN Document Server

Schwartz, Ezra

2014-01-01

A hands-on guide filled with practical examples to device/OS independent User Experience Strategy with Axure 7. If you are a UX practitioner or a business analyst or a product manager involved in UX projects, this book is for you. This book will also be useful to consultants or in-house staff who work for agencies, and individual practitioners or UX team members. Familiarity with Axure will help but is not mandatory.

Cell adhesion and proliferation on polyethylene grafted with Au nanoparticles

Czech Academy of Sciences Publication Activity Database

Kasálková-Slepičková, N.; Slepička, P.; Kolská, Z.; Sajdl, P.; Bačáková, Lucie; Rimpelová, S.; Švorčík, V.

2012-01-01

Roč. 272, FEB 1 (2012), s. 391-395 ISSN 0168-583X. [International Conference on Ion Beam Modification of Materials /17./. Montreal, 22.08.2010-27.08.2010] R&D Projects: GA ČR(CZ) GAP108/10/1106; GA AV ČR(CZ) KAN400480701 Institutional research plan: CEZ:AV0Z50110509 Keywords : polyenthyne * gold nanoparticles * grafting * cell proliferation Subject RIV: EI - Biotechnology ; Bionics Impact factor: 1.266, year: 2012

Warming-induced upward migration of the alpine treeline in the Changbai Mountains, northeast China

Czech Academy of Sciences Publication Activity Database

Du, H.; Liu, J.; Li, M.; Büntgen, Ulf; Yang, Y.; Wang, L.; Wu, Z.; He, Hong S.

2018-01-01

Roč. 24, č. 3 (2018), s. 1256-1266 ISSN 1354-1013 R&D Projects: GA MŠk(CZ) LO1415 Institutional support: RVO:86652079 Keywords : climate - change * tibetan plateau * pinus-cembra * elevation * dynamics * shifts * forest * growth * line * carbon * altitudinal transect * Betula ermanii * Changbai Mountains * climate change * dendroecology * forest growth * treeline dynamics Subject RIV: EH - Ecology, Behaviour OBOR OECD: Environmental sciences (social aspects to be 5.7) Impact factor: 8.502, year: 2016

Stability Indicating RP-HPLC Method for Determination of Valsartan in Pure and Pharmaceutical Formulation

Directory of Open Access Journals (Sweden)

S. K. Patro

2010-01-01

Full Text Available A simple, rapid and accurate and stability indicating RP-HPLC method was developed for the determination of valsartan in pure and tablet forms. The method showed a linear response for concentrations in the range of 50-175 µg/mL using 0.01 M NH4H2PO4 (pH 3.5 buffer: methanol [50:50] as the mobile phase with detection at 210 nm and a flow rate of 1 mL/min and retention time 11.041 min. The method was statistically validated for accuracy, precision, linearity, ruggedness, robustness, forced degradation, solution stability and selectivity. Quantitative and recovery studies of the dosage form were also carried out and analyzed; the % RSD from recovery studies was found to be less than 1. Due to simplicity, rapidity and accuracy of the method, we believe that the method will be useful for routine quality control analysis.

Scientific Opinion on the Pest Risk Analysis on Phytophthora ramorum prepared by the FP6 project RAPRA

DEFF Research Database (Denmark)

Baker, R.; Candresse, T.; Dormannsné Simon, E.

2011-01-01

The Panel on Plant Health was asked to deliver a scientific opinion on the Pest Risk Analysis on Phytophthora ramorum prepared by the FP6 project RAPRA, taking into account comments by Member States and additional information since RAPRA. P. ramorum is the oomycete causing sudden oak death...

Properties of made by different methods of RP impeller foundry patterns

Directory of Open Access Journals (Sweden)

G. Budzik

2007-04-01

Full Text Available This article presents the tests of properties of foundry patterns of turbocharger turbine impeller. Impellers prototypes were predestinated for casting by method losing patterns. There were carried out tests of these prototypes made by different methods of Rapid Prototyping (RP. Two impeller were made by growth methods: stereolitography (SLA and three dimensional printing (3DP. One prototype was made by the method of Vacuum Casting as a wax casting. Tests were executed in the Department of Machine Design of Rzeszow University of Technology in cooperation with WSK PZL Rzeszow and Car Technology Krakow. First impeller was carried out by method of stereolitography on SLA 250 plant. That pattern was also used to carry out silicon matrix for casting of wax pattern. Next pattern was printed by three dimensional printer Z510 from the powder ZP14. Good removability of the pattern from the mould is particularly essential for impellers of small turbines with blades of small thickness of their section. All pattern were tested on their removability from the ceramic mould. The best melting properties had the wax pattern. Patterns made from resin SL5170 (SLA and powder ZP14 (3DP were removed in the process of burning but about 1% of soot was left in the mould.

Application of CAD/CFD/RP-technologies for development of LVAD systems

Directory of Open Access Journals (Sweden)

Morozov Valentin

2016-01-01

Full Text Available The important problem of new Left Ventricle Assist Device (LVAD systems development is to determine their hemodynamic and functional characteristics and reduce the design time. An effective method for achieving this is the use of approaches based on the application of CAD/CFD/PR-technologies. Methods of computer modelling were applied to determine the geometrical parameters of the blood pump, and methods of finite element analysis were used for the optimization of hemodynamic parameters of the LVAD system. The geometrical modelling was carried out in the Pro/Engineer WF5. The CAE-system Ansys was used to analyze the blood flow in the chamber of the pump. The motion of the flexible walls was simulated by normal flow through rigid walls. At the final stage some models were prepared for prototyping. The critical parameters of hemodynamic gave the possibility to optimize the shape of the blood pump. The LVAD system and its components were made using various RP-technologies. The hydraulic tests for the performance investigation and the analysis of implantability for implantable systems were carried out for manufactured prototypes of LVAD systems. The manufactured components of the LVAD system are completely operability and give the possibility to estimate the critical hemodynamic and functional parameters of the LVAD system.

6 sigma quality performance

International Nuclear Information System (INIS)

Yu, Yeong Hak

2000-03-01

This deals with 6 sigma quality performance introducing company which has 6 sigma quality management, 6 sigma quality activity and customer, secret of success of 6 sigma quality management, what 6 sigma is, 6 sigma quality management propel system 5 propel steps of project like point of 6 sigma, flow of problem solution, tool for propel of project, performance of CTQ and total customer satisfaction, and quality management system and 6 sigma quality.

Final Technical Report on STTR Project DE-FG02-06ER86282 Development and Demonstration of 6-Dimensional Muon Beam Cooling

Energy Technology Data Exchange (ETDEWEB)

Muons, Inc.

2011-05-24

The overarching purpose of this project was to prepare a proposal for an experiment to demonstrate 6-dimensional muon beam cooling. The technical objectives were all steps in preparing the proposal, which was successfully presented to the Fermilab Accelerator Advisory Committee in February 2009. All primary goals of this project have been met.

Synthesis and characterization of Ruddlesden-Popper (RP) type phase LaSr2MnCrO7

International Nuclear Information System (INIS)

Singh, Devinder; Singh, Rajinder

2010-01-01

New Ruddlesden-Popper (RP) type phase LaSr 2 MnCrO 7 has been synthesized by ceramic method. Rietveld profile analysis shows that the phase crystallizes with tetragonal unit cell in the space group 14/mmm. The electrical resistivity of the phase has been measured in the temperature range of 10-300 K using Leybold closed cycle helium cryostat. The phase shows insulator-metal (I-M) transition at low temperature, the phenomenon often associated with giant magnetoresistance. 3D variable range hopping governs the electrical conduction in the insulator region above the I-M transition temperature. Magnetic susceptibility of the phase has been measured in the temperature range of 100-300 K. Magnetic studies suggest that the phase is ferromagnetic. (author)

AgRP and NPY Expression in the Human Hypothalamic Infundibular Nucleus Correlate with Body Mass Index, Whereas Changes in alpha MSH Are Related to Type 2 Diabetes

NARCIS (Netherlands)

Alkemade, Anneke; Yi, Chun-Xia; Pei, Lei; Harakalova, Magdalena; Swaab, Dick F.; la Fleur, Susanne E.; Fliers, Eric; Kalsbeek, Andries

2012-01-01

Context: Rodent data show that altered hypothalamic signaling contributes to the development of obesity and insulin resistance. Objective: To determine differences in hypothalamic expression levels of neuropeptide Y (NPY), agouti-related peptide (AgRP), and alpha MSH in the infundibular nucleus, the

EU Framework 6 Project: Predictive Toxicology (PredTox)-overview and outcome

International Nuclear Information System (INIS)

Suter, Laura; Schroeder, Susanne; Meyer, Kirstin; Gautier, Jean-Charles; Amberg, Alexander; Wendt, Maria; Gmuender, Hans; Mally, Angela; Boitier, Eric; Ellinger-Ziegelbauer, Heidrun; Matheis, Katja; Pfannkuch, Friedlieb

2011-01-01

In this publication, we report the outcome of the integrated EU Framework 6 Project: Predictive Toxicology (PredTox), including methodological aspects and overall conclusions. Specific details including data analysis and interpretation are reported in separate articles in this issue. The project, partly funded by the EU, was carried out by a consortium of 15 pharmaceutical companies, 2 SMEs, and 3 universities. The effects of 16 test compounds were characterized using conventional toxicological parameters and 'omics' technologies. The three major observed toxicities, liver hypertrophy, bile duct necrosis and/or cholestasis, and kidney proximal tubular damage were analyzed in detail. The combined approach of 'omics' and conventional toxicology proved a useful tool for mechanistic investigations and the identification of putative biomarkers. In our hands and in combination with histopathological assessment, target organ transcriptomics was the most prolific approach for the generation of mechanistic hypotheses. Proteomics approaches were relatively time-consuming and required careful standardization. NMR-based metabolomics detected metabolite changes accompanying histopathological findings, providing limited additional mechanistic information. Conversely, targeted metabolite profiling with LC/GC-MS was very useful for the investigation of bile duct necrosis/cholestasis. In general, both proteomics and metabolomics were supportive of other findings. Thus, the outcome of this program indicates that 'omics' technologies can help toxicologists to make better informed decisions during exploratory toxicological studies. The data support that hypothesis on mode of action and discovery of putative biomarkers are tangible outcomes of integrated 'omics' analysis. Qualification of biomarkers remains challenging, in particular in terms of identification, mechanistic anchoring, appropriate specificity, and sensitivity.

Environmental Science: 49 Science Fair Projects. Science Fair Projects Series.

Science.gov (United States)

Bonnet, Robert L.; Keen, G. Daniel

This book contains 49 science fair projects designed for 6th to 9th grade students. Projects are organized by the topics of soil, ecology (projects in habitat and life cycles), pests and controls (projects in weeds and insects), recycling (projects in resources and conservation), waste products (projects in decomposition), microscopic organisms,…

Development of Isocratic RP-HPLC Method for Separation and Quantification of L-Citrulline and L-Arginine in Watermelons

Directory of Open Access Journals (Sweden)

Rasdin Ridwan

2018-01-01

Full Text Available Watermelons (Citrullus lanatus are known to have sufficient amino acid content. In this study, watermelons grown and consumed in Malaysia were investigated for their amino acid content, L-citrulline and L-arginine, by the isocratic RP-HPLC method. Flesh and rind watermelons were juiced, and freeze-dried samples were used for separation and quantification of L-citrulline and L-arginine. Three different mobile phases, 0.7% H3P04, 0.1% H3P04, and 0.7% H3P04 : ACN (90 : 10, were tested on two different columns using Zorbax Eclipse XDB-C18 and Gemini C18 with a flow rate of 0.5 mL/min and a detection wavelength at 195 nm. Efficient separation with reproducible resolution of L-citrulline and L-arginine was achieved using 0.1% H3P04 on the Gemini C18 column. The method was validated and good linearity of L-citrulline and L-arginine was obtained with R2 = 0.9956, y=0.1664x+2.4142 and R2=0.9912, y=0.4100x+3.4850, respectively. L-citrulline content showed the highest concentration in red watermelon of flesh and rind juice extract (43.81 mg/g and 45.02 mg/g, whereas L-arginine concentration was lower than L-citrulline, ranging from 3.39 to 11.14 mg/g. The isocratic RP-HPLC method with 0.1% H3P04 on the Gemini C18 column proved to be efficient for separation and quantification of L-citrulline and L-arginine in watermelons.

Radiation Protection Group Annual Report 2003

CERN Document Server

Silari, M

2004-01-01

The RP Annual Report summarises the activities carried out by CERN’s Radiation Protection Group in the year 2003. It includes contribution from the EN section of the TIS/IE Group on environmental monitoring. Chapter 1 reports on the measurements and estimations of the impact on the environment and public exposure due to the Organisation’s activities. Chapter 2 provides the results of the monitoring of CERN’s staff, users and contractors to occupational exposure. Chapter 3 deals with operational radiation protection around the accelerators and in the experimental areas. Chapter 4 reports on RP design studies for the LHC and CNGS projects. Chapter 5 addresses the various services provided by the RP Group to other Groups and Divisions at CERN, which include managing radioactive waste, high-level dosimetry, lending radioactive test sources and shipping radioactive materials. Chapter 6 describes activities other than the routine and service tasks, i.e. development work in the field of instrumentation and res...

[Project EXCEL: Curriculum for Just Desserts, Inc.: Modules 1-6.

Science.gov (United States)

Career Resources Development Center, Inc., San Francisco, CA.

Six curriculum modules for Just Desserts, a San Francisco wholesale bakery company, are presented. Just Desserts was one of four small businesses involved in Project EXCEL, a workplace literacy project that focused on literacy and basic skills training for limited-English-proficient (LEP) workers. The modules are as follows: (1) Bakery Vocabulary…

Secure Research at scale

Directory of Open Access Journals (Sweden)

Simon Thompson

2017-04-01

The demand for UKSeRP has been higher than initially envisaged and the possible use cases are constantly being imagined. We are also introducing an entry level UKSeRP to support individual projects and their requirements.

Development and validation of a stability-indicating RP-HPLC method for assay of betamethasone and estimation of its related compounds.

Science.gov (United States)

Fu, Qiang; Shou, Minshan; Chien, Dwight; Markovich, Robert; Rustum, Abu M

2010-02-05

Betamethasone (9alpha-fluoro-16beta-methylprednisolone) is one of the members of the corticosteriod family of active pharmaceutical ingredient (API), which is widely used as an anti-inflammatory agent and also as a starting material to manufacture various esters of betamethasone. A stability-indicating reverse-phase high performance liquid chromatography (RP-HPLC) method has been developed and validated which can separate and accurately quantitate low levels of 26 betamethasone related compounds. The stability-indicating capability of the method was demonstrated through adequate separation of all potential betamethasone related compounds from betamethasone and also from each other that are present in aged and stress degraded betamethasone stability samples. Chromatographic separation of betamethasone and its related compounds was achieved by using a gradient elution at a flow rate of 1.0mL/min on a ACE 3 C18 column (150mmx4.6mm, 3microm particle size, 100A pore size) at 40 degrees C. Mobile phase A of the gradient was 0.1% methanesulfonic acid in aqueous solution and mobile phase B was a mixture of tert-butanol and 1,4-dioxane (7:93, v/v). UV detection at 254nm was employed to monitor the analytes. For betamethasone 21-aldehyde, the QL and DL were 0.02% and 0.01% respectively. For betamethasone and the rest of the betamethasone related compounds, the QL and DL were 0.05% and 0.02%. The precision of betamethasone assay is 0.6% and the accuracy of betamethasone assay ranged from 98.1% to 99.9%.

Development and Validation of RP-HPLC Method for the Determination of Adefovir Dipivoxil in Bulk and in Pharmaceutical Formulation

Directory of Open Access Journals (Sweden)

Zaheer Ahmed

2009-01-01

Full Text Available A rapid and sensitive RP-HPLC method with UV detection (262 nm for routine analysis of adefovir dipivoxil in bulk and in pharmaceutical formulation was developed. Chromatography was performed with mobile phase containing a mixture of acetonitrile and phosphate buffer (50:50, v/v with flow rate 1.0 mL min-l. In the range of 5.0-100 µg/mL, the linearity of adefovir dipivoxil shows a correlation co-efficient of 0.9999. The proposed method was validated by determining sensitivity accuracy, precision, robustness stability, specificity, selectivity and system suitability parameters.

Ghrelin Agonist JMV 1843 Increases Food Intake, Body Weight and Expression of Orexigenic Neuropeptides in Mice

Czech Academy of Sciences Publication Activity Database

Holubová, Martina; Špolcová, Andrea; Demianova, Zuzana; Sýkora, D.; Fehrentz, J. A.; Martinez, J.; Štofková, A.; Jurčovičová, J.; Drápalová, J.; Lacinová, Z.; Haluzík, M.; Železná, Blanka; Maletínská, Lenka

2013-01-01

Roč. 62, č. 4 (2013), s. 435-444 ISSN 0862-8408 R&D Projects: GA ČR GA303/09/0744; GA ČR GAP303/10/1368 Institutional support: RVO:61388963 Keywords : GHS-R agonists * JMV 1843 * male C57BL/6 mice * food intake * NPY/AgRP Subject RIV: CE - Biochemistry Impact factor: 1.487, year: 2013

Multivariate analysis of elution parameters for RP-HPLC with charged aerosol detection of sucrose caprate regioisomers

DEFF Research Database (Denmark)

Lie, Aleksander; Pedersen, Lars Haastrup

2012-01-01

and Environmental Engineering, Aalborg University, Denmark Sugar fatty acid monoesters have been shown to possess antibiotic and insecticidal properties. The physical and chemical properties of sugar fatty acid esters depend on the saccharide moiety, fatty acid chain length, and both position and degree...... for sucrose caprate regioisomers. As a sensitive method based on mass detection, charged aerosol detection was used. The investigation was conducted using design-of-experiments (DOE) methodology for development and prediction of elution strategies. The elution profiles were described by a number of important......BIOCAT2012, Hamburg University of Technology Book of Abstracts, p290 ISBN 987-3-941492 L73) Multivariate analysis of elution parameters for RP-HPLC with charged aerosol detection of sucrose caprate regioisomers Aleksander Lie and Lars Haastrup Pedersen Department of Biotechnology, Chemistry...

Portable linear accelerator development. Final report

International Nuclear Information System (INIS)

Schonberg, R.G.

1983-01-01

The final report on Project RP 822-6 describes the MINAC 3 development from the recognition of need for a lightweight, portable high-energy device to the successful completion and field use of the MINAC. MINAC, which represents a substantial improvement in field radiographic capability and in technology, rapidly transitioned from proof-of-principle (1978 to 1980) to field-proven product (1980 to 1981). As a result, the decision has been made to develop this report as a users guide as well as a historic record of design, development, and testing program. The first section of this report has the following principal objectives: to describe the existing MINAC equipment capabilities and achievable modifications; to provide applications information for prospective users; and to provide technical information on high-energy radiography useful for familiarization and planning. The users guide section is followed by sections which describe the design basis, development, and application history of MINAC through the course of EPRI RP822 research projects 1 to 5, inclusively

A Study of Method Development, Validation, and Forced Degradation for Simultaneous Quantification of Paracetamol and Ibuprofen in Pharmaceutical Dosage Form by RP-HPLC Method

OpenAIRE

Jahan, Md. Sarowar; Islam, Md. Jahirul; Begum, Rehana; Kayesh, Ruhul; Rahman, Asma

2014-01-01

A rapid and stability-indicating reversed phase high-performance liquid chromatography (RP-HPLC) method was developed for simultaneous quantification of paracetamol and ibuprofen in their combined dosage form especially to get some more advantages over other methods already developed for this combination. The method was validated according to United States Pharmacopeia (USP) guideline with respect to accuracy, precision, specificity, linearity, solution stability, robustness, sensitivity, and...

Development and Validation of a Stability-Indicating RP-HPLC Method for Rapid Determination of Doxycycline in Pharmaceutical Bulk and Dosage Forms

OpenAIRE

Shabnam Pourmoslemi, Soroush Mirfakhraee, Saeid Yaripour, Ali Mohammadi

2016-01-01

Background: A rapid stability-indicating RP-HPLC method for analysis of doxycycline in the presence of its degradation products was developed and validated. Methods: Forced degradation studies were carried out on bulk samples and capsule dosage forms of doxycycline using acid, base, H2O2, heat, and UV light as described by ICH for stress conditions to demonstrate the stability-indicating power of the method. Separations were performed on a Perfectsil® Target ODS column (3-5µm, 125 mm×4 mm), u...

Statistical optimization of an RP-HPLC method for the determination of selected flavonoids in berry juices and evaluation of their antioxidant activities.

Science.gov (United States)

Ciric, Andrija; Jelikic-Stankov, Milena; Cvijovic, Milica; Djurdjevic, Predrag

2018-04-01

An isocratic RP-HPLC method for the separation and identification of selected flavonoids (quercetin, rutin, luteolin-7-O-glucoside, kaempferol and kaempferol-3-O-glucoside) in commercial berry juices (blackcurrant, blueberry, red raspberry and cherry) was developed with the aid of central composite design and response surface methodology. The optimal separation conditions were a mobile phase of 85:15 (% v/v) water-acetonitrile, pH 2.8 (adjusted with formic acid), flow rate 0.5 mL min -1 and column temperature 35°C. The obtained levels of bioflavonoids (mg per 100 mL of juice) were as follows: for quercetin, ca. 0.21-5.12; for kaempferol, ca. 0.05-1.2; for rutin, ca. 0.4-6.5; for luteolin-7-O-glucoside, ca. 5.6-10.2; and for kaempferol-3-O-glucoside, ca. 0.02-0.12. These are considerably lower than the values in fresh fruits. Total phenolic, flavonoid and anthocyanin contents were determined spectrophotometrically. Total flavonoid content varied as follows: blackcurrant > blueberry > red raspberry > cherry. The antioxidant activity of juice extracts (DPPH and ABTS methods) expressed as IC 50 values varied from 8.56 to 14.05 mg L -1 . These values are ~2.5-3 times lower than quercetin, ascorbic acid and Trolox®, but compared with rutin and butylhydroxytoluene, berries show similar or better antioxidant activity by both the DPPH and ABTS methods. Copyright © 2017 John Wiley & Sons, Ltd.

All projects related to Bangladesh | Page 6 | IDRC - International ...

International Development Research Centre (IDRC) Digital Library (Canada)

Livelihood Diversification for Smallholder Tobacco Farmers in South Nyanza, Kenya - Phase I. Project ... Program: Networked Economies ... LAW, MIGRATION POLICY, IMMIGRATION LAW, WOMEN'S RIGHTS, GENDER EQUALITY, Gender.

Effect of relative position of the control rods in the power calibration of the running chains of the RP-10 nuclear reactor

International Nuclear Information System (INIS)

Zuniga, Agustin; Arrieta, Rolando

2014-01-01

The monitoring of the thermal power levels of an experimental nuclear reactor, such as the RP-10 reactor is performed by current levels reported by the driving chains and their correlation with the power. The calibration curves for each channel will depend on the neutron distribution observed for the corresponding configuration. One way to modify this configuration is positioning the control rods that can be chosen according to the convenience of providing greater neutron flux in a given irradiation position. This article shows this variation, and consequently the current power curves corresponding thereto. We have chosen two relative positions of the control rods (BC1 and BC2), the first is the one that favors the production of radioisotopes (PPR) (Case 1) and the second favors the use of neutron activation (NAA) (Case 2). The used method was based on the record of the current (up camera, M4) by the acquisition system (SAD) of the reactor and the power from the digital reading of nitrogen 16, which was previously calibrated by thermal balance. The results for the nuclear configuration 30, M4 chain up were: Case 1: I M4 (A) = 2.95 x 10 -6 x P (MW) + 1.45 x 10 -6 R 2 = 9.99 x 10 -1 and Case 2: I M4 (A) = 2.39 x 10 -6 x P (MW) + 5.73 x 10 -7 , R 2 = 9.97 x 10 -1 . The dispersions of the data are in the order of 10 % for low power (under 2 MW), decreasing as the power rises to under 3 % (for values greater than 3 MW). (authors).

Project descriptions

International Nuclear Information System (INIS)

Anon.

1990-01-01

This part specifies the activities and project tasks of each project broken down according to types of financing, listing the current projects Lw 1 through 3 funded by long-term provisions (budget), the current projects LB 1 and 2, LG 1 through 5, LK1, LM1, and LU 1 through 6 financed from special funds, and the planned projects ZG 1 through 4 and ZU 1, also financed from special funds. (DG) [de

The One Plan Project: A cooperative effort of the National Response Team and the Region 6 Regional Response Team to simplify facility emergency response planning

International Nuclear Information System (INIS)

Staves, J.; McCormick, K.

1997-01-01

The National Response Team (NRT) in coordination with the Region 6 Response Team (RRT) have developed a facility contingency plan format which would integrate all existing regulatory requirements for contingency planning. This format was developed by a multi-agency team, chaired by the USEPA Region 6, in conjunction with various industry, labor, and public interest groups. The impetus for this project came through the USEPA Office of Chemical Emergency Preparedness and Prevention (CEPPO). The current national oil and hazardous material emergency preparedness and response system is an amalgam of federal, state, local, and industrial programs which are often poorly coordinated. In a cooperative effort with the NRT, the CEPPO conducted a Presidential Review of federal agency authorities and coordination responsibilities regarding release prevention, mitigation, and response. Review recommendations led to a Pilot Project in USEPA Region 6. The Region 6 Pilot Project targeted end users in the intensely industrialized Houston Ship Channel (HSC) area, which is comprised of petroleum and petrochemical companies

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Involuntary resettlement: A cross-country study on urban inequality and poverty. Project ... Topic: AGRICULTURAL PRODUCTION, ZOONOSES, GENDER ANALYSIS, Disease control, ... Preparing States in India for Universal Health Coverage.

Drugs for stroke: action of nitrone (Z)-N-(2-bromo-5-hydroxy-4-methoxybenzylidene)-2-methylpropan-2-amine oxide on rat cortical neurons in culture subjected to oxygen-glucose-deprivation.

Science.gov (United States)

Arce, Carmen; Diaz-Castroverde, Sabela; Canales, María J; Marco-Contelles, José; Samadi, Abdelouahid; Oset-Gasque, María J; González, María P

2012-09-01

The action of (Z)-N-(2-bromo-5-hydroxy-4-methoxybenzylidene)-2-methylpropan-2-amine oxide (RP6) on rat cortical neurons in culture, under oxygen-glucose-deprivation conditions, is reported. Cortical neurons in culture were treated during 1 h with OGD. After, they were placed under normal conditions during 24 h (reperfusion) in absence and presence of RP6. Different parameters were measured under each condition (control, 1 h OGD and 1 h OGD + reperfusion in absence and presence of RP6). RP6 protects neurons against ROS generation, lipid peroxidation levels, LDH release and mitochondrial membrane potential alteration, when administered during reperfusion after the OGD damage. Consequently, these results show that nitrone RP6 protects cells against ischemia injury produced during the reoxygenation, and could be a potential drug for the ictus therapy. Copyright © 2012. Published by Elsevier Masson SAS.

Development, Validation and Application of RP-HPLC Method: Simultaneous Determination of Antihistamine and Preservatives with Paracetamol in Liquid Formulations and Human Serum

OpenAIRE

Hasan, Najmul; Chaiharn, Mathurot; Toor, Umair Ali; Mirani, Zulfiqar Ali; Sajjad, Ghulam; Sher, Nawab; Aziz, Mubashir; Siddiqui, Farhan Ahmed

2016-01-01

In this article we describe development and validation of stability indicating, accurate, specific, precise and simple Ion-pairing RP-HPLC method for simultaneous determination of paracetamol and cetirizine HCl along with preservatives i.e. propylparaben, and methylparaben in pharmaceutical dosage forms of oral solution and in serum. Acetonitrile: Buffer: Sulfuric Acid (45:55:0.3 v/v/v) was the mobile phase at flow rate 1.0 mL min-1 using a Hibar? Lichrosorb? C18 column and monitored at wavel...

Development and Validation of a Simultaneous RP-HPLCUV/DAD Method for Determination of Polyphenols in Gels Containing S. terebinthifolius Raddi (Anacardiaceae)

Science.gov (United States)

Carvalho, Melina G.; Aragão, Cícero F. S; Raffin, Fernanda N.; de L. Moura, Túlio F. A.

2017-01-01

Topical gels containing extracts of Schinus terebinthifolius have been used to treat bacterial vaginosis. It has been reported that this species has antimicrobial, anti-inflammatory and anti-ulcerogenic properties, which can be attributed to the presence of phenolic compounds. In this work, a sensitive and selective reversed-phase HPLC-UV/DAD method for the simultaneous assay of six polyphenols that could be present in S. terebinthifolius was developed. The method was shown to be accurate and precise. Peak purity and similarity index both exceeded 0.99. Calibration curves were linear over the concentration range studied, with correlation coefficients between 0.9931 and 0.9974. This method was used to determine the polyphenol content of a hydroalcoholic extract and pharmacy-compounded vaginal gel. Although the method is useful to assess the 6 phenolic compounds, some compounds could not be detected in the products. SUMMARY A sensitive, selective, accurate and precise reversed-phase HPLC-UV/DAD method for the simultaneous assay of six polyphenols in S. terebinthifolius Raddi Abbreviations used: RP-HPLC-UV/DAD: Reverse Phase High Performance Liquid Chromatograph with Ultraviolet and Diode Array Detector, HPLC: High Performance Liquid Chromatograph, HPLC-UV: High Performance Liquid Chromatograph with Ultraviolet Detector, ANVISA: Brazilian National Health Surveillance Agency, LOD: Limit of detection, LOQ: Limit of quantitation PMID:28539726

Emergency automatic commutation of the ventilation system of the RP-10 nuclear reactor

International Nuclear Information System (INIS)

Castillo, Walter; Corimanya, Mario; Ovalle, Edgar; Anaya, Olgger; Veramendi, Emilio

2013-01-01

The present paper summarizes the achievements in the design and implementation of a system for monitoring and automatic control of radioactive effluents from the chimney of the RP-10 reactor, using as hardware an Arduino UNO platform containing an ATMEGA 328 programmable micro controller to which has been added LCD screen to display the values, a keyboard and an EEPROM memory data, where the limit of the level of radiation is fixed. The radiation level in the air of the reactor hall, going up the chimney is counted by a radiation monitor called MAB1000, and data are supplied to the new system. When the radiation level is above the national and international standards, the new design makes work a relay, so that the ventilation system is automatically switched to operate in emergency condition, preventing the release of radioactive contaminants into the environment. After installing the new design, it was verified that removed by the radiation monitor MAB1000, value is identical to that shown in the new system. Additionally, the operation of the relay was tested successfully with radioactive sources to switch the ventilation system to the emergency condition. (authors).

Bioinspired Surface Treatments for Improved Decontamination: Polymer Based SlipperyLiquid Infused Porous Surfaces (SLIPS)

Science.gov (United States)

2018-04-23

Materials & Interfaces 6, 1502-1508 (2014). 2. M.A. Samaha; M. Gad-el-Hak, " Polymeric Slippery Coatings: Nature and Applications," Polymers 6, 1266...organic polymers producing chains that can serve to reinforce the overall structure. The POSS variants evaluated under this study included a cage structure...cage structures of silicon and oxygen that bind to organic polymers producing large chains that can act as a reinforcing fiber (Figure 1). They are

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International Development Research Centre (IDRC) Digital Library (Canada)

Program: Agriculture and Food Security. Total Funding: ... Technologies for Social Inclusion and Public Policies in Latin America. Project ... Region: Argentina, South America, Brazil, Chile, Peru, Uruguay, North and Central America. Program: ...

Rapid Aggregation of Biofuel-Producing Algae by the Bacterium Bacillus sp. Strain RP1137

Science.gov (United States)

Powell, Ryan J.

2013-01-01

Algal biofuels represent one of the most promising means of sustainably replacing liquid fuels. However, significant challenges remain before alga-based fuels become competitive with fossil fuels. One of the largest challenges is the ability to harvest the algae in an economical and low-energy manner. In this article, we describe the isolation of a bacterial strain, Bacillus sp. strain RP1137, which can rapidly aggregate several algae that are candidates for biofuel production, including a Nannochloropsis sp. This bacterium aggregates algae in a pH-dependent and reversible manner and retains its aggregation ability after paraformaldehyde fixation, opening the possibility for reuse of the cells. The optimal ratio of bacteria to algae is described, as is the robustness of aggregation at different salinities and temperatures. Aggregation is dependent on the presence of calcium or magnesium ions. The efficiency of aggregation of Nannochloropsis oceanica IMET1 is between 70 and 95% and is comparable to that obtained by other means of harvest; however, the rate of harvest is fast, with aggregates forming in 30 s. PMID:23892750

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International Development Research Centre (IDRC) Digital Library (Canada)

Region: Brazil, South America, Colombia, Haiti, North and Central America, Mexico. Program: Governance ... Open Data for Public Policy in Latin America and the Caribbean. Project ... Program: Food, Environment, and Health. Total Funding: ...

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International Development Research Centre (IDRC) Digital Library (Canada)

Improving and Securing Women's Access to Land in Sénégal. Project ... Topic: LAND TENURE, GENDER DISCRIMINATION, GENDER EQUALITY, Gender ... Institutionalizing Gender and Women's Rights and Citizenship at Cheikh Anta Diop ...

Comprehensive Two-Dimensional Hydrophilic Interaction Chromatography (HILIC) × Reversed-Phase Liquid Chromatography Coupled to High-Resolution Mass Spectrometry (RP-LC-UV-MS) Analysis of Anthocyanins and Derived Pigments in Red Wine.

Science.gov (United States)

Willemse, Chandré M; Stander, Maria A; Vestner, Jochen; Tredoux, Andreas G J; de Villiers, André

2015-12-15

Changes in anthocyanin chemistry represent some of the most important transformations involved in red wine aging. However, accurate analysis of the derived pigments, as required to study the evolution of anthocyanins and tannins during aging, is hampered by their extreme structural diversity, low levels, and the fact that many of these compounds have identical mass spectral characteristics. In this context, chromatographic separation is critical. In this contribution, the application of online hydrophilic interaction chromatography (HILIC) × reversed-phase liquid chromatography (RP-LC) separation coupled to high-resolution mass spectrometry (MS) is described for the detailed characterization of anthocyanins and their derived pigments in aged red wine. A systematic approach was followed for the optimization of HILIC × RP-LC separation parameters using a capillary liquid chromatography (LC) system in the first dimension and an ultrahigh-pressure LC system in the second dimension to ensure maximum sensitivity and performance. Ninety four (94) anthocyanin-derived pigments were tentatively identified in one- and six-year-old Pinotage wines using accurate mass and fragmentation information obtained using quadrupole-time-of-flight mass spectrometry (Q-TOF-MS). Online HILIC × RP-LC-MS was found to offer high-resolution separation, because of the combination of two different separation modes, while the structured elution order observed improved the certainty in compound identification. Therefore, this approach shows promise for the detailed elucidation of the chemical alteration of anthocyanins during wine aging.

Intergrated metabonomic study of the effects of Guizhi Fuling capsule intervention on primary dysmenorrheal using RP-UPLC-MS complementary with HILIC-UPLC-MS technique.

Science.gov (United States)

Lang, Lang; Meng, Zhaorui; Sun, Lan; Xiao, Wei; Zhao, Longshan; Xiong, Zhili

2018-02-01

Guizhi Fuling capsule (GFC), developed from the traditional Chinese prescription of Guizhi Fuling Wan, has been commonly used for the treatment of primary dysmenorrhea (PD). However, the intervention effective mechanism in vivo has not been well elucidated. In this study, an integrated plasma metabonomic strategy based on RP-UPLC-MS coupled with HILIC-UPLC-MS technique has been developed to investigate the global therapeutic effects and intervention mechanisms of GFC on dysmenorrhea rats induced by oxytocin. The 20 potential biomarkers were identified and primarily related to sphingolipid metabolism, steroid hormone biosynthesis, glycerophospholipid metabolism, amino acid metabolism, lipid metabolism and energy metabolism. The results showed that the GFC has therapeutic effects on rats with dysmenorrhea via the regulation of multiple metabolic pathways. Some new potential biomarkers associated with primary dysmenorrhea such as phenylalanine, tryptophan, taurine, carnitine, betaine, creatine and creatinine have been discovered in this study for the first time. This study provides a metabonomic platform based on RP-UPLC-MS complementary to HILIC-UPLC-MS technique to investigate both nonpolar and polar compounds, so as to get a more comprehensive metabolite information to yield insight into the pathophysiology of PD and assessing the efficacy of GFC on PD rats. Copyright © 2017 John Wiley & Sons, Ltd.

Project B-610 instrument data base. Revision 6

International Nuclear Information System (INIS)

Silvan, G.R.

1994-01-01

The following technical information document contains the published data base listing for the instrumentation to be connected into the new MICON distributed control computer. Project B-610 PFP Instrument Upgrade (PFP HVAC Control Room Upgrade) will install the MICON system in PFP and provide for a new control room at the present site of the new SWP change room EDT-150750 1/11/93

Development and validation of simple RP-HPLC-PDA analytical protocol for zileuton assisted with Design of Experiments for robustness determination

Directory of Open Access Journals (Sweden)

Saurabh B. Ganorkar

2017-02-01

Full Text Available A simple, rapid, sensitive, robust, stability-indicating RP-HPLC-PDA analytical protocol was developed and validated for the analysis of zileuton racemate in bulk and in tablet formulation. Development of method and resolution of degradation products from forced; hydrolytic (acidic, basic, neutral, oxidative, photolytic (acidic, basic, neutral, solid state and thermal (dry heat degradation was achieved on a LC – GC Qualisil BDS C18 column (250 mm × 4.6 mm × 5 μm by isocratic mode at ambient temperature, employing a mobile phase methanol and (0.2%, v/v orthophosphoric acid in ratio of (80:20, v/v at a flow rate of 1.0 mL min−1 and detection at 260 nm. ‘Design of Experiments’ (DOE employing ‘Central Composite Design’ (CCD and ‘Response Surface Methodology’ (RSM were applied as an advancement to traditional ‘One Variable at Time’ (OVAT approach to evaluate the effects of variations in selected factors (methanol content, flow rate, concentration of orthophosphoric acid as graphical interpretation for robustness and statistical interpretation was achieved with Multiple Linear Regression (MLR and ANOVA. The method succeeded over the validation parameters: linearity, precision, accuracy, limit of detection and limit of quantitation, and robustness. The method was applied effectively for analysis of in-house zileuton tablets.

Analysis of iridoids, secoiridoids and xanthones in Centaurium erythraea, Frasera caroliniensis and Gentiana lutea using LC-MS and RP-HPLC.

Science.gov (United States)

Aberham, Anita; Pieri, Valerio; Croom, Edward M; Ellmerer, Ernst; Stuppner, Hermann

2011-02-20

This study presents a new and validated HPLC method for the simultaneous determination of bioactive compounds in Centaurium erythraea, Frasera caroliniensis and Gentiana lutea. The iridoid loganic acid, four secoiridoids and 29 xanthones were separated on a RP-18 column, using aqueous o-phosphoric acid (0.085%, v/v) and acetonitrile as mobile phase. Phytochemical investigation of C. erythraea herb and F. caroliniensis roots resulted into isolation of 25 xanthones and three secoiridoids the structure of which was elucidated by spectroscopic means (NMR, MS and UV). 1,3,8-Trihydroxy-5,6-dimethoxyxanthone, isolated from C. erythraea, turned out to be a novel xanthone. The stability of the analytes was tested by subjecting samples to light, moisture and different temperatures. After six months of storage, decomposition of gentiopicroside and sweroside was observed. The swertiamarin content was nearly unchanged when stored at room temperature or in the refrigerator, but high temperature conditions reduced the content to 85%. In contrast, xanthones were stable under long-term, refrigerated and accelerated conditions. The established chromatographic method has been successfully applied for the quantification of the bioactive compounds in the three plants. The presence and distribution of polyoxygenated xanthones within the three members of the Gentianaceae family and their significance as analytical markers are discussed. Copyright © 2010 Elsevier B.V. All rights reserved.

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International Development Research Centre (IDRC) Digital Library (Canada)

All projects related to China ... crop improvement and natural resource management are under stress. ... The vast majority of workers in India, and an increasing share of the labour force in China, work under conditions described as informal.

Radon and lung cancer in the Ardennes and Eifel region

International Nuclear Information System (INIS)

Wichmann, H.E.; Poffijn, C.

1993-01-01

The objectives of the project are to perform epidemiological studies on the role of radon in the etiology of lung cancer in the Ardennes-Eifel region and in Brittany. In each of the participating countries, Belgium, France, Germany and Luxemburg cases and controls were collected in a series of hospitals. The radon exposure for the last 35 years was reconstructed through 6 months measurements in the living and bedrooms of the different dwellings. The objectives and results of the eight contributions to the project for the reporting period are presented. (R.P.) 1 ref

A PEX6-defective peroxisomal biogenesis disorder with severe phenotype in an infant, versus mild phenotype resembling Usher syndrome in the affected parents.

Science.gov (United States)

Raas-Rothschild, Annick; Wanders, Ronald J A; Mooijer, Petra A W; Gootjes, Jeannette; Waterham, Hans R; Gutman, Alisa; Suzuki, Yasuyuki; Shimozawa, Nobuyuki; Kondo, Naomi; Eshel, Gideon; Espeel, Marc; Roels, Frank; Korman, Stanley H

2002-04-01

Sensorineural deafness and retinitis pigmentosa (RP) are the hallmarks of Usher syndrome (USH) but are also prominent features in peroxisomal biogenesis defects (PBDs); both are autosomal recessively inherited. The firstborn son of unrelated parents, who both had sensorineural deafness and RP diagnosed as USH, presented with sensorineural deafness, RP, dysmorphism, developmental delay, hepatomegaly, and hypsarrhythmia and died at age 17 mo. The infant was shown to have a PBD, on the basis of elevated plasma levels of very-long- and branched-chain fatty acids (VLCFAs and BCFAs), deficiency of multiple peroxisomal functions in fibroblasts, and complete absence of peroxisomes in fibroblasts and liver. Surprisingly, both parents had elevated plasma levels of VLCFAs and BCFAs. Fibroblast studies confirmed that both parents had a PBD. The parents' milder phenotypes correlated with relatively mild peroxisomal biochemical dysfunction and with catalase immunofluorescence microscopy demonstrating mosaicism and temperature sensitivity in fibroblasts. The infant and both of his parents belonged to complementation group C. PEX6 gene sequencing revealed mutations on both alleles, in the infant and in his parents. This unique family is the first report of a PBD with which the parents are themselves affected individuals rather than asymptomatic carriers. Because of considerable overlap between USH and milder PBD phenotypes, individuals suspected to have USH should be screened for peroxisomal dysfunction.

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International Development Research Centre (IDRC) Digital Library (Canada)

Topic: ELECTRONIC PUBLISHING, RESEARCH RESULTS, PERIODICALS. Region: South of Sahara, Ghana, Kenya, Nigeria, Uganda, Tanzania, South Africa. Total Funding: CA$ 128,000.00. Association of African Universities : Education and Research Networking Unit. Project. The Association of African Universities ...

The Majorana project: sup 7 sup 6 Ge 0 nu beta beta-decay neutrino mass measurement

CERN Document Server

Aalseth, C E

2002-01-01

Interest in, and the relevance of, next-generation 0 nu beta beta-decay experiments is increasing. Even with nonzero neutrino mass strongly suggested by SNO, Super Kamiokande, and similar experiments sensitive to delta m sup 2 , 0 nu beta beta-decay experiments are still the only way to establish the Dirac or Majorana nature of neutrinos by measuring effective electron neutrino mass, . Various theorists have recently argued in favor of a neutrino mass between 0.01 and 1 eV. The Majorana Project aims to probe this effective neutrino mass range, reaching a sensitivity of 0.02-0.07 eV. The experiment relies entirely on proven technology and has been devised based upon the materials, technology, and data analysis demonstrated to produce the lowest background per kilogram of fiducial germanium. The project plan includes 500 kg of germanium detector material enriched to 85% in sup 7 sup 6 Ge, specialized pulse-acquisition electronics and detector segmentation for background rejection, and underground electroformed ...

Doubling in the use of thyroid hormone replacement therapy in Denmark: association to iodization of salt?

DEFF Research Database (Denmark)

Cerqueira, Charlotte; Knudsen, Nils; Ovesen, Lars

2011-01-01

increased 75% in the moderately iodine deficient region (72.2 incident users/100,000 person-years in 1997 to 126.6 in 2008) and 87% in the mildly deficient region (86.9–162.9). When stratified by sex and age-group (00–39, 40–64, 65+) the largest relative increase was seen among women in the youngest age...

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... INFORMATION TECHNOLOGY, ENVIRONMENTAL PROTECTION. Region: South Asia, Central Asia, Far East Asia, India, Nepal, Pakistan. Program: Agriculture and Food Security. Total Funding: CA$ 1,019,600.00. Learning Initiatives for Network Economies in Asia (LIRNEasia) : Building Capacity in ICT Policy. Project.

Status and results from the decay spectroscopy project EURICA (Euroball-RIKEN cluster array)

Energy Technology Data Exchange (ETDEWEB)

Söderström, P.-A., E-mail: pasoder@ribf.riken.jp; Doornenbal, P.; Nishimura, S.; Baba, H.; Isobe, T.; Kiss, G. [RIKEN Nishina Center, 2-1 Hirosawa, Wako-shi, Saitama 351-0198 (Japan); Lorusso, G. [RIKEN Nishina Center, 2-1 Hirosawa, Wako-shi, Saitama 351-0198 (Japan); National Physical Laboratory, Teddington, Middlesex, TW11 0LW (United Kingdom); Wu, J. [RIKEN Nishina Center, 2-1 Hirosawa, Wako-shi, Saitama 351-0198 (Japan); School of Physics, Peking University, Beijing 100871 (China); Xu, Z. Y. [Department of Physics, University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-0033 (Japan); KU Leuven, Instituut voor Kern-en Stralingsfysica, 3001 Leuven (Belgium); Benzoni, G. [Istituto Nazionale di Fisica Nucleare, Sezione di Milano, Via Celoria 16, 20133 Milano (Italy); Browne, F. [RIKEN Nishina Center, 2-1 Hirosawa, Wako-shi, Saitama 351-0198 (Japan); School of Computing, Engineering and Mathematics, University of Brighton, Brighton BN2 4JG (United Kingdom); Gey, G. [RIKEN Nishina Center, 2-1 Hirosawa, Wako-shi, Saitama 351-0198 (Japan); LPSC, Université Grenoble-Alpes, CNRS/IN2P3, F-38026 Grenoble Cedex (France); ILL, 38042 Grenoble Cedex (France); Research Center for Nuclear Physics (RCNP), Osaka University, Ibaraki, Osaka 567-0047 (Japan); Jungclaus, A. [Instituto de Estructura de la Materia, CSIC, E-28006 Madrid (Spain); Kojouharov, I.; Kurz, N.; Schaffner, H. [GSI Helmholtzzentrum für Schwerionenforschung GmbH, 64291 Darmstadt (Germany); Lubos, D. [RIKEN Nishina Center, 2-1 Hirosawa, Wako-shi, Saitama 351-0198 (Japan); Physik Department E12, Technische Universität München, D-85748 Garching (Germany); Moschner, K. [RIKEN Nishina Center, 2-1 Hirosawa, Wako-shi, Saitama 351-0198 (Japan); IKP, University of Cologne, D-50937 Cologne (Germany); and others

2016-07-07

β- and isomer-decay spectroscopy are sensitive probes of nuclear structure, and are often the only techniques capable of providing data for exotic nuclei that are produced with very low rates. Decay properties of exotic nuclei are also essential to model astrophysical events responsible for the evolution of the universe such as the rp- and r-processes. The EURICA project (EUROBALL RIKEN Cluster Array) has been launched in 2012 with the goal of performing spectroscopy of very exotic nuclei. Since 2012, five experimental campaigns have been successfully completed using fragmentation of {sup 124}Xe beam and in-flight-fission of {sup 238}U beam. In these proceedings we will introduce the experimental setup and highlight some key recent results around {sup 78}Ni, {sup 132}Sn, and {sup 110}Zn published during 2014 and 2015.

All projects related to colombia | Page 6 | IDRC - International ...

International Development Research Centre (IDRC) Digital Library (Canada)

Longer life expectancy and lower fertility rates have accelerated the ageing of the ... Topic: DEMOGRAPHIC TRANSITION, ECONOMIC IMPLICATIONS, SOCIAL ... Land Seizures in Northern Colombia and their effect on the Peasant Movement ... This project aims to build telecentre networks that are financially sustainable ...

Quantitative analysis of iridoids, secoiridoids, xanthones and xanthone glycosides in Gentiana lutea L. roots by RP-HPLC and LC-MS.

Science.gov (United States)

Aberham, Anita; Schwaiger, Stefan; Stuppner, Hermann; Ganzera, Markus

2007-11-05

The here described HPLC-method enables the determination of all major, currently known bioactive compounds in gentian roots. A separation of iridoids (loganic acid), secoiridoids (swertiamarin, gentiopicroside, amarogentin, sweroside), xanthones (gentisin, isogentisin) and two xanthone glycosides (gentiosides) was possible on RP-18 column material, using 0.025% aqueous TFA, acetonitrile and n-propanol as mobile phase. The method is sensitive (LODlutea samples showed that gentiopicroside is the most dominant compound in the specimens (4.46-9.53%), followed by loganic acid (0.10-0.76%), swertiamarin (0.21-0.45%) and the xanthone glycosides. Gentisin and isogentisin were found in much lower concentrations between 0.02 and 0.11%, respectively.

Stability-Indicating RP-HPLC Method for Determination of Guanfacine Hydrochloride in Bulk Drugs and in Pharmaceutical Dosage Form

Directory of Open Access Journals (Sweden)

Vinod K. Ahirrao

2011-04-01

Full Text Available A novel stability-indicating RP-HPLC method was developed and validated for quantitative determination of guanfacine hydrochloride in bulk drug and in pharmaceutical dosage form. An isocratic, reversed phase HPLC method was developed to separate the drug from the degradation products, using Apollo, C18 (250mm x 4.6mm, 5µm column with mobile phase of 50mM Ammonium acetate (volatile buffer and acetonitrile (65:35, v/v. UV detection has been done at wavelength 220 nm. The guanfacine hydrochloride was subjected to the stress conditions of hydrolysis (acid, base, oxidation, photolysis and thermal degradation. The stressed samples were analyzed by the proposed method. The analyte peak shape was excellent. The described method shows excellent linearity over a range of 30 – 450 µg/mL. The correlation coefficient for guanfacine hydrochloride was 0.999. The limit of detection for Guanfacine hydrochloride is 0.011 µg/mL and the limit of quantification is 0.038 µg/mL respectively.Degradation was observed for guanfacine hydrochloride in base, thermal and in 30% H2O2 conditions. The drug was found to be stable in the other stress conditions attempted. The degradation products were well resolved from main peak. The percentage recovery of guanfacine hydrochloride was ranged from (99.2% to 100.5% in pharmaceutical dosage form. The developed method was validated with respect to the linearity, accuracy (recovery, precision, specificity and robustness. The forced degradation studies prove the stability indicating power of the method.

Project management. A discipline which contributes to project success

International Nuclear Information System (INIS)

Hoch, G.

2008-01-01

The presentation covers the following topics: description of the Project - a contract was signed between KNPP and European Consortium Kozloduy (ECK) consisting of Framatome ANP GmbH as Leader (63%), Framatome ANP S.A.S. (17%), Atomenergoexport (20%) ; Project management in the modernization of NPP Kozloduy units 5 and 6; Project management process within AREVA NP GmbH; current status

Biodegradable polymeric nanoformulation based on the antiprotozoal canthin-6-one

International Nuclear Information System (INIS)

Arias, José L.; Cebrián-Torrejón, Gerardo; Poupon, Erwan; Fournet, Alain; Couvreur, Patrick

2011-01-01

The efficacy of antiprotozoal agents against intracellular infections is very often limited by an almost negligible access to the cellular level where the pathogens are hidden. As a result, high doses of the chemotherapy agents are needed to be administered, but the great incidence of severe adverse drug effects generally leads to pharmacotherapy failure. To enhance the pharmacological effect of the antiprotozoal and antifungal canthin-6-one, loading into biodegradable poly(octylcyanoacrylate) nanoparticles has been considered. The preparation of canthin-6-one nanoformulation (average size ≈170 nm) has been performed by a single-absorption procedure with high drug loading and little burst release as determined by RP-HPLC. Further characterization of this nanoformulation has been carry out by electrophoretic measurements, analysis of the surface thermodynamics of the nanoparticles, and 1 H-NMR analysis. Nanoparticles loaded with canthin-6-one were characterized by a significant hydrophobicity and a great surface electrical charge under physiological conditions. These are two key physicochemical factors determining recognition by the reticuloendothelial system, resulting in a fast intracellular uptake by infected phagocytes. It is expected that this nanoformulation offers potential applications for an efficient canthin-6-one delivery to intracellular infections.

All projects related to | Page 6 | IDRC - International Development ...

International Development Research Centre (IDRC) Digital Library (Canada)

This pilot project is one of four pilots exploring the feasibility of a Think Heath Initiative, a prospective program that would support evidence-based policy engagement on the health-related Sustainable Development Goals (SDGs). Region: South Asia, Bangladesh, Bhutan, Sri Lanka, India, Nepal, Pakistan, Afghanistan.

Study of the 17Ne Coulomb dissociation process and its role for the rp process of nucleosynthesis

Energy Technology Data Exchange (ETDEWEB)

Marganiec, Justyna [TU Darmstadt (Germany); EMMI, GSI Darmstadt (Germany); Wamers, Felix [EMMI, GSI Darmstadt (Germany); TU Darmstadt (Germany); GSI Darmstadt (Germany); FIAS, Frankfurt am Main (Germany); Aumann, Thomas [TU Darmstadt (Germany); GSI Darmstadt (Germany); Egorova, Irina [BLTP JINR Dubna (Russian Federation); Grigorenko, Leonid [FLNR JINR Dubna (Russian Federation); RRC KI, Moscow (Russian Federation); Heil, Michael [GSI Darmstadt (Germany); Perfenova, Yuliya [FLNR JINR Dubna (Russian Federation); INP, Moscow (Russian Federation); Plag, Ralf [GSI Darmstadt (Germany); Goethe-Universitaet, Frankfurt am Main (Germany); Collaboration: R3B-Collaboration

2014-07-01

The study of {sup 17}Ne Coulomb dissociation process gives us a possibility to study the time-reversed reaction {sup 15}O(2p,γ){sup 17}Ne, with the detailed balance theorem. This reaction could serve as a bypass of {sup 15}O waiting point during the rp process, and move the initial CNO material towards heavier nuclei. The two-proton capture can proceed sequentially or directly from the three-body continuum. And the reaction rate can be enhanced by a few orders of magnitude by taking the three-body continuum into account. The Coulomb dissociation method is the one way to experimentally determine the three-body radiative capture cross section, which is needed to verify theoretical calculations, and which was not experimentally determined yet. The experiment has been performed at the LAND/R3B setup at GSI.

Homozygosity mapping in autosomal recessive retinitis pigmentosa families detects novel mutations

Science.gov (United States)

Marzouka, Nour al Dain; Hebrard, Maxime; Manes, Gaël; Sénéchal, Audrey; Meunier, Isabelle; Hamel, Christian P.

2013-01-01

Purpose Autosomal recessive retinitis pigmentosa (arRP) is a genetically heterogeneous disease resulting in progressive loss of photoreceptors that leads to blindness. To date, 36 genes are known to cause arRP, rendering the molecular diagnosis a challenge. The aim of this study was to use homozygosity mapping to identify the causative mutation in a series of inbred families with arRP. Methods arRP patients underwent standard ophthalmic examination, Goldman perimetry, fundus examination, retinal OCT, autofluorescence measurement, and full-field electroretinogram. Fifteen consanguineous families with arRP excluded for USH2A and EYS were genotyped on 250 K SNP arrays. Homozygous regions were listed, and known genes within these regions were PCR sequenced. Familial segregation and mutation analyzes were performed. Results We found ten mutations, seven of which were novel mutations in eight known genes, including RP1, IMPG2, NR2E3, PDE6A, PDE6B, RLBP1, CNGB1, and C2ORF71, in ten out of 15 families. The patients carrying RP1, C2ORF71, and IMPG2 mutations presented with severe RP, while those with PDE6A, PDE6B, and CNGB1 mutations were less severely affected. The five families without mutations in known genes could be a source of identification of novel genes. Conclusions Homozygosity mapping combined with systematic screening of known genes results in a positive molecular diagnosis in 66.7% of families. PMID:24339724

All projects related to Uganda | Page 6 | IDRC - International ...

International Development Research Centre (IDRC) Digital Library (Canada)

Region: Uganda, North of Sahara, South of Sahara. Program: Think Tank Initiative. Total Funding: CA$ 1,845,170.00. Institutional Support : Makerere Institute of Social Research (MISR). Project. Makerere Institute of Social Research (MISR), Uganda, is the former East African Institute of Social and Economic Research ...

CERN radiation protection (RP) calibration facilities

Energy Technology Data Exchange (ETDEWEB)

Pozzi, Fabio

2016-04-14

, the facility was commissioned by measuring the calibration quantities of interest, e.g. H*(10), as a function of the source-to-detector distance. In the case of neutron measurements, a comparison with the Monte Carlo results was carried out; in fact, the neutron scattering can be an important issue and the Monte Carlo method can contribute to its estimation and optimization. Neutron calibrations often need to be performed at neutron energies or spectra very much different from those generated by radioactive sources employed in standard calibration laboratories. Unfortunately, fields with a broad neutron spectrum extending to a few GeVs are very rare and the scientific community is calling for worldwide sharing of the existing facilities. The CERN RP group has been managing the CERN-EU high-energy Reference Field (CERF) facility for 20 years, which is a unique calibration field in its kind. CERF is a workplace field that reproduces the neutron spectrum encountered in the vicinity of high-energy accelerators and at commercial flight altitudes. Within the context of providing a well-characterized workplace field to the scientific community, Monte Carlo simulations were performed with the present development version of the FLUKA code. The simulations were compared with experimental measurements showing promising results for the future ISO accreditation of the facility as workplace reference facility. Even though the accreditation process is fairly long, the work achieved so far is setting the bases to start this process in the right way.

Time Delay for the Initiation of an Emergency Shutdown at the Peruvian Nuclear Reactor RP-10

International Nuclear Information System (INIS)

Ramon, A.; Ovalle, E.; Canaza, D.; Salazar, A.; Zapata, A.; Felix, J.; Arrieta, R.; Vela, M.

2008-01-01

In this paper we show the measurement of the time delay for the initiation of an emergency shutdown state at the RP-10 Reactor. This time delay is the one corresponding to the delay between the detection of a signal of any fixed limit and the start of a protective action to get the reactor in a safety state. The experimental method used is based on monitoring two signals in an oscilloscope, one signal is the elected initiate event and the other is the de-energizing of electromagnets of the security bars. The time delay for each safety and control rods, was measured for seven energizing current values in a range of 36 - 52 mA. The results showed that the minimum value is (84 ± 1.26) ms and the maximum is (108 ± 1.60) ms. In all cases it is noted that, the delay time is less than the limit values prefixed down in the reactor safety report. (authors)

FY 1999 report on the results of the contract project 'The model project for facilities for effective utilization of industrial waste at the industrial complex in Thailand.' Separate Volume 6 - FY 1999 project; 1999 nendo seika hokokusho. Tai ni okeru kogyo danchi sangyo haikibutsu yuko riyo setsubi moderu jigyo - 6

Energy Technology Data Exchange (ETDEWEB)

NONE

2001-03-01

For the purpose of reducing the consumption of fossil fuel by recycling industrial waste for effective use as petroleum substituting energy in Thailand where the amount of industrial waste is expected to increase, a model project on facilities for effective use of industrial waste at the industrial complex was carried out, and the FY 1999 results were reported. Concretely, the industrial waste generated from each plant at the industrial complex owned by IEAT is to be incinerated in fluidized bed incinerator, and the process steam is to be generated by recovering waste heat by waste heat recovery boiler and to be supplied to plants within the complex. In this fiscal year, the first year of the project, the attachment to the agreement was prepared in terms of the allotment of the project work between Japan and Thailand, various kinds of gist, schedules, etc. and signed. After that, the following were conducted at the Japan side according to the attachment to the agreement: determination of the basic specifications for facilities, basic design, detailed design, manufacture of a part of the equipment, etc. Separate Volume 6 included drawings of assembling of the equipment such as crane, crusher and valve. (NEDO)

Glucose Alters Per2 Rhythmicity Independent of AMPK, Whereas AMPK Inhibitor Compound C Causes Profound Repression of Clock Genes and AgRP in mHypoE-37 Hypothalamic Neurons.

Directory of Open Access Journals (Sweden)

Johanneke E Oosterman

Full Text Available Specific neurons in the hypothalamus are regulated by peripheral hormones and nutrients to maintain proper metabolic control. It is unclear if nutrients can directly control clock gene expression. We have therefore utilized the immortalized, hypothalamic cell line mHypoE-37, which exhibits robust circadian rhythms of core clock genes. mHypoE-37 neurons were exposed to 0.5 or 5.5 mM glucose, comparable to physiological levels in the brain. Per2 and Bmal1 mRNAs were assessed every 3 hours over 36 hours. Incubation with 5.5 mM glucose significantly shortened the period and delayed the phase of Per2 mRNA levels, but had no effect on Bmal1. Glucose had no significant effect on phospho-GSK3β, whereas AMPK phosphorylation was altered. Thus, the AMPK inhibitor Compound C was utilized, and mRNA levels of Per2, Bmal1, Cryptochrome1 (Cry1, agouti-related peptide (AgRP, carnitine palmitoyltransferase 1C (Cpt1c, and O-linked N-acetylglucosamine transferase (Ogt were measured. Remarkably, Compound C dramatically reduced transcript levels of Per2, Bmal1, Cry1, and AgRP, but not Cpt1c or Ogt. Because AMPK was not inhibited at the same time or concentrations as the clock genes, we suggest that the effect of Compound C on gene expression occurs through an AMPK-independent mechanism. The consequences of inhibition of the rhythmic expression of clock genes, and in turn downstream metabolic mediators, such as AgRP, could have detrimental effects on overall metabolic processes. Importantly, the effects of the most commonly used AMPK inhibitor Compound C should be interpreted with caution, considering its role in AMPK-independent repression of specific genes, and especially clock gene rhythm dysregulation.

Glucose Alters Per2 Rhythmicity Independent of AMPK, Whereas AMPK Inhibitor Compound C Causes Profound Repression of Clock Genes and AgRP in mHypoE-37 Hypothalamic Neurons.

Science.gov (United States)

Oosterman, Johanneke E; Belsham, Denise D

2016-01-01

Specific neurons in the hypothalamus are regulated by peripheral hormones and nutrients to maintain proper metabolic control. It is unclear if nutrients can directly control clock gene expression. We have therefore utilized the immortalized, hypothalamic cell line mHypoE-37, which exhibits robust circadian rhythms of core clock genes. mHypoE-37 neurons were exposed to 0.5 or 5.5 mM glucose, comparable to physiological levels in the brain. Per2 and Bmal1 mRNAs were assessed every 3 hours over 36 hours. Incubation with 5.5 mM glucose significantly shortened the period and delayed the phase of Per2 mRNA levels, but had no effect on Bmal1. Glucose had no significant effect on phospho-GSK3β, whereas AMPK phosphorylation was altered. Thus, the AMPK inhibitor Compound C was utilized, and mRNA levels of Per2, Bmal1, Cryptochrome1 (Cry1), agouti-related peptide (AgRP), carnitine palmitoyltransferase 1C (Cpt1c), and O-linked N-acetylglucosamine transferase (Ogt) were measured. Remarkably, Compound C dramatically reduced transcript levels of Per2, Bmal1, Cry1, and AgRP, but not Cpt1c or Ogt. Because AMPK was not inhibited at the same time or concentrations as the clock genes, we suggest that the effect of Compound C on gene expression occurs through an AMPK-independent mechanism. The consequences of inhibition of the rhythmic expression of clock genes, and in turn downstream metabolic mediators, such as AgRP, could have detrimental effects on overall metabolic processes. Importantly, the effects of the most commonly used AMPK inhibitor Compound C should be interpreted with caution, considering its role in AMPK-independent repression of specific genes, and especially clock gene rhythm dysregulation.

Simultaneous and accurate determination of water- and fat-soluble vitamins in multivitamin tablets by using an RP-HPLC method

Directory of Open Access Journals (Sweden)

Semahat Kucukkolbasi

2013-01-01

Full Text Available In the present study, a reversed-phase high-performance liquid chromatographic (RP-HPLC procedure was developed and validated for the simultaneous determination of seven water-soluble vitamins (thiamine, riboflavin, niacin, cyanocobalamin, ascorbic acid, folic acid, and p-aminobenzoic acid and four fat-soluble vitamins (retinol acetate, cholecalciferol, α-tocopherol, and phytonadione in multivitamin tablets. The linearity of the method was excellent (R² > 0.999 over the concentration range of 10 - 500 ng mL-1. The statistical evaluation of the method was carried out by performing the intra- and inter-day precision. The accuracy of the method was tested by measuring the average recovery; values ranged between 87.4% and 98.5% and were acceptable quantitative results that corresponded with the label claims.

Primary processing of CRISPR RNA by the endonuclease Cas6 in Staphylococcus epidermidis.

Science.gov (United States)

Wakefield, Noelle; Rajan, Rakhi; Sontheimer, Erik J

2015-10-07

In many bacteria and archaea, an adaptive immune system (CRISPR-Cas) provides immunity against foreign genetic elements. This system uses CRISPR RNAs (crRNAs) derived from the CRISPR array, along with CRISPR-associated (Cas) proteins, to target foreign nucleic acids. In most CRISPR systems, endonucleolytic processing of crRNA precursors (pre-crRNAs) is essential for the pathway. Here we study the Cas6 endonuclease responsible for crRNA processing in the Type III-A CRISPR-Cas system from Staphylococcus epidermidis RP62a, a model for Type III-A CRISPR-Cas systems, and define substrate requirements for SeCas6 activity. We find that SeCas6 is necessary and sufficient for full-length crRNA biogenesis in vitro, and that it relies on both sequence and stem-loop structure in the 3' half of the CRISPR repeat for recognition and processing. Copyright © 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Determining the specific activity of thymidine phosphorylase in leukocytes of patients with MNGIE and the plasma thymidine level by RP-HPLC

Directory of Open Access Journals (Sweden)

Rezaei Sh

2011-06-01

Full Text Available "nBackground: Thymidine phosphorylase (TP catalyses the conversion of thymidine into thymine. Mitochondrial neurogastrointestinal encephalomyopathy (MNGIE is an autosomal recessive disease which is caused by mutations in the nuclear gene encoding TP, bringing about severe impairment of TP-enzyme specific activity and accumulation of thymidine in plasma. The clinical manifestations of MNGIE are recognizable and homogenous, but not in the early stages of the disease. In patients who are suspected of having MNGIE, determination of TP-specific activity in leukocytes and thymidine levels in plasma are diagnostic. The methods that are usually used for the measurement of TP activity and plasma thymidine are not rapid or accurate enough and lack sensitivity."n "nMethods: The specific activity of TP was measured by RP-HPLC in leukocytes of both the controls and the patients exhibiting clinical features suggestive of MNGIE. Moreover, plasma thymidine was assessed by the same method."n "nResults: The patients had detectable plasma thymidine (>3 µmol/L but it was undetectable in the healthy controls. The patients' TP-specific activity decreased to less than 5% relative to the controls (14±4 nmol/h/mg vs. 525±165 nmol/h/mg, P<0.05. A diagnostic algorithm for the definitive diagnosis of MNGIE is suggestible based on the results of this study which relies on the measurement of plasma thymidine, TP-specific activity in leukocytes, or both."n "nConclusion: In this study, we set up a sensitive and rapid assay for the evaluation of TP-specific activity by using RP-HPLC in Iran. In addition, we established reference values for TP-specific activity and plasma thymidine in the Iranian patients.

Strong association between a splice mutation (IVS12+5G{r_arrow}A) and haplotype 6 in hereditary tyrosinemia type I

Energy Technology Data Exchange (ETDEWEB)

Tanguay, R.M.; St-Louis, M.; Gibson, K. [Universite Laval, Ste-Foy (Canada)] [and others

1994-09-01

Hereditary tyrosinemia type I (HT I; McKusick 276700) is a severe inborn error of tyrosine catabolism pathway caused by a deficiency of fumarylacetoacetate hydrolase (FAH). The highest frequency reported is the one in Saguenay-Lac St-Jean (Quebec, Canada) where 1:1,846 births are affected. The FAH gene has been cloned and several mutations have been described. Allele specific oligonucleotide (ASO) hybridization was used to examine the frequency of a splice (IVS12-5G{r_arrow}A) mutation recently reported and RFLP analysis was done to identify haplotypes related to HT I. The splice mutation was found on 45/50 alleles (90%) in patients from SLSJ and 12/66 (18%) alleles from patients world-wide. All 25 patients from the SLSJ region were positive with 20 being homozygous, indicating that this mutation is the major cause of HT I in French Canada. Of these 25 patients, 96% were positive for one haplotype called no 6 which is these 25 patients, 96% were positive for one haplotype called no 6 which is identified by TaqI, RsaI, BglII, MspI and KpnI digestions. These data show a really strong association between the mutation (IVS12+5G{r_arrow}A) and haplotype 6. Among our patients from around the world, {approximately}52% were positive for haplotype 6 indicating its strong relation with HT I. These results provide the rationale for DNA-based carrier testing for HT I in the F-C population at risk as well as in HT I patients in general.

RP-HPLC Analysis of Quercetin in the Extract of Sambong (Blumea balsamifera (L DC Leaves

Directory of Open Access Journals (Sweden)

Joanna V. Toralba

2015-06-01

Full Text Available Blumea balsamifera (L DC, known in the Philippines as sambong, is an herb valued for its health benef its especially in the management of urolithiasis. Various phytochemicals, including flavonoids such as quercetin, have been determined in sambong leaves. A reversed-phase high-performance liquid chromatographic method (RP-HPLC was developed for the quantitative determination of quercetin in the methanol extract of sambong leaves obtained from Leyte, Cotabato, and Nueva Ecija, Philippines. The methanol extracts of sambong were prepared by maceration followed by rotary evaporation. The solid phase extraction (SPE for the sample cleanup involved the use of a C18 SPE packing, a 0.5-mL sample load (50 mg/mL solution, and elution with 4-mL of 80:20 Methanol:0.5% H3PO4. The HPLC conditions for the determination of quercetin involved the use of a C18 4.6-mm x 250-mm column maintained at 30°C, 254-nm UV detection, and a mobile phase composition of 25 parts methanol and 75 parts mixture of 0.5% H3PO4 and 0.2% triethylamine with a 1 mL/min flow rate in gradient elution. A good linearity at the concentration range of 3.72–124 μg/mL of quercetin standard (r2=0.9989 was observed with the limits of detection (LOD and quantitation (LOQ at 0.68 ng/mL and 2.28 ng/mL, respectively. The intra-day (n=5- and inter-day (n=3 precision values were satisfactory (%RSD <2%. The recovery eff iciency of the SPE sample cleanup step, which was checked by spiking sambong solution with quercetin standard, was 102.41%. The quercetin contents are 0.2337mg, 0.1350mg, and 0.2940mg per gram of the powdered dried leaves of sambong from Nueva Ecija, Cotabato, and Leyte, respectively. This is the f irst report of quercetin content in the leaves of sambong collected from the Philippines.

A validated method using RP-HPLC for quantification of reserpine in the Brazilian tree Rauvolfia sellowii Müll. Arg. (Apocynaceae

Directory of Open Access Journals (Sweden)

Leopoldo C. Baratto

2012-01-01

Full Text Available This study describes a simple, fast and reproducible method using RP-HPLC-UV, in a gradient system, for quantification of reserpine in Rauvolfia sellowii stem bark. The analysis were carried out on a C18 column; mobile phase was water and acetonitrile, and separations were carried out in 10 min, flow rate of 1.0 mL min-1, 25 ºC and 268 nm. The validation data showed that the method was specific, accurate, precise and robust. Results were linear over a range of 0.625-40.0 μg mL-1, and the mean recovery was 95.1%. The amount of reserpine found in the dried stem bark was 0.01% (m/m.

Increase of apatite dissolution rate by Scots pine roots associated or not with Burkholderia glathei PML1(12)Rp in open-system flow microcosms

Science.gov (United States)

Calvaruso, Christophe; Turpault, Marie-Pierre; Frey-Klett, Pascale; Uroz, Stéphane; Pierret, Marie-Claire; Tosheva, Zornitza; Kies, Antoine

2013-04-01

The release of nutritive elements through apatite dissolution represents the main source of phosphorus, calcium, and several micronutrients (e.g., Zn, Cu) for organisms in non-fertilized forest ecosystems. The aim of this study was to quantify, for the first time, the dissolution rate of apatite grains by tree roots that were or were not associated with a mineral weathering bacterial strain, and by various acids known to be produced by tree roots and soil bacterial strains in open-system flow microcosms. In addition, we explored whether the mobilization of trace elements (including rare earth elements) upon apatite dissolution was affected by the presence of trees and associated microorganisms. The dissolution rate of apatite by Scots pine plants that were or were not inoculated with the strain Burkholderia glathei PML1(12)Rp, and by inorganic (nitric) and organic (citric, oxalic and gluconic) acids at pH 5.5, 4.8, 3.8, 3.5, 3.0, and 2.0 was monitored in two controlled experiments: "plant-bacteria interaction" and "inorganic and organic acids". Analyses of the outlet solutions in the "plant-bacteria interaction" experiment showed that Scots pine roots and B. glathei PML1(12)Rp produced protons and organic acids such as gluconate, oxalate, acetate, and lactate. The weathering budget calculation revealed that Scots pines (with or without PML1(12)Rp) significantly increased (factor > 10) the release of Ca, P, As, Sr, Zn, U, Y, and rare earth elements such as Ce, La, Nd from apatite, compared to control abiotic treatment. Scanning electron microscopy observation confirmed traces of apatite dissolution in contact of roots. Most dissolved elements were taken up by Scots pine roots, i.e., approximately 50% of Ca, 70% of P, 30% of As, 70% of Sr, 90% of Zn, and 100% of U, Y, and rare earth elements. Interestingly, no significant additional effect due to the bacterial strain PML1(12)Rp on apatite dissolution and Scots pine nutrition and growth was observed. The "inorganic

Science communication in European projects

International Nuclear Information System (INIS)

Vachev, Boyko; Stamenov, Jordan

2009-01-01

Science communication in several resent successful projects of Institute for Nuclear Research and Nuclear Energy, Bulgarian Academy of Sciences (INRNE, BAS) from the 5th and 6th Framework Programmes of EC is presented: the joint INRNE, BAS project with JRC of EC (FP5 NUSES) and two subsequent Centre of Excellence projects (FP5 HIMONTONET and FP6 BEOBAL) are considered. Innovations and traditional forms development and application are discussed. An overview of presentation and communication of INRNE, BAS contribution to Bulgarian European Project is made. Good practices have been derived. Keywords: Science communication, European projects, Innovations

Efficient preparation of incensole and incensole acetate, and quantification of these bioactive diterpenes in Boswellia papyrifera by a RP-DAD-HPLC method.

Science.gov (United States)

Paul, Michael; Jauch, Johann

2012-03-01

Incensole and incensole acetate, found in incense, are encouraging potent bioactive diterpenic cembrenoids, inhibiting Nuclear Factor-kappaB activation. Furthermore, incensole acetate elicits psycho-activity in mice by activating the TRPV3 channels in the brain. Starting from crude extracts of the incense species Boswellia papyrifera Hochst., a convenient procedure for the efficient large-scale synthesis of incensole and its acetate is presented. Additionally, a reversed-phase, diode-array-detection, high-performance liquid chromatography (RP-DAD-HPLC) method for the quantification of incensole and incensole acetate is reported, indicating that these two compounds are typical biomarkers for B. papyrifera.

PUB22 and PUB23 U-BOX E3 ligases directly ubiquitinate RPN6, a 26S proteasome lid subunit, for subsequent degradation in Arabidopsis thaliana

DEFF Research Database (Denmark)

Cho, Seok Keun; Bae, Hansol; Ryu, Moonyoung

2015-01-01

and PUB23, two U-box E3 ligase homologs, tether ubiquitins to 19S proteasome regulatory particle (RP) subunit RPN6, leading to its degradation. RPN6 was identified as an interacting substrate of PUB22 by yeast two-hybrid screening, and in vitro pull-down assay confirmed that RPN6 interacts not only......Drought stress strongly affects plant growth and development, directly connected with crop yields, accordingly. However, related to the function of U-BOX E3 ligases, the underlying molecular mechanisms of desiccation stress response in plants are still largely unknown. Here we report that PUB22...

Logic based feature detection on incore neutron spectra

International Nuclear Information System (INIS)

Bende-Farkas, S.; Kiss, S.; Racz, A.

1992-09-01

A methodology is proposed to investigate neutron spectra in such a way which is similar to human thinking. The goal was to save experts from tedious, mechanical tasks of browsing a large amount of signals in order to recognize changes in the underlying mechanisms. The general framework for detecting features of incore neutron spectra with a rulebased methodology is presented. As an example, the meaningful peaks in the APSDs are determined. This method is a part of a wider project to develop a noise diagnostic expert system. (R.P.) 6 refs.; 6 figs.; 1 tab

A PEX6-defective peroxisomal biogenesis disorder with severe phenotype in an infant, versus mild phenotype resembling Usher syndrome in the affected parents

NARCIS (Netherlands)

Raas-Rothschild, Annick; Wanders, Ronald J. A.; Mooijer, Petra A. W.; Gootjes, Jeannette; Waterham, Hans R.; Gutman, Alisa; Suzuki, Yasuyuki; Shimozawa, Nobuyuki; Kondo, Naomi; Eshel, Gideon; Espeel, Marc; Roels, Frank; Korman, Stanley H.

2002-01-01

Sensorineural deafness and retinitis pigmentosa (RP) are the hallmarks of Usher syndrome (USH) but are also prominent features in peroxisomal biogenesis defects (PBDs); both are autosomal recessively inherited. The firstborn son of unrelated parents, who both had sensorineural deafness and RP

The Llama Project.

Science.gov (United States)

Ganzel, Candy; Stuglik, Jan

2003-01-01

At a suburban Indiana elementary school, the Project Approach serves as the basis of the curriculum in all Kindergarten classrooms. The four classes of 5- and 6-year-old children at this school chose to study llamas. This article discusses how the project evolved, describes the three phases of the project, and provides teachers' reflections on the…

Ukraine - USA partnership for peace. AEMS project on development and improving of 'Defense' automated emergency management system of the Civil Defense Department of Ukraine

International Nuclear Information System (INIS)

1998-01-01

AEMS project is destined for the development and improving of 'Defense' system of the Civil Defense Department of Ukraine. 'Defense' system is destined for prevention and operation prognosis of emergencies of technological and natural character on the territory of Ukraine as well as for the elaboration of the solutions on protection and rescue of the population and national economy resources. The data are processed at national, regional, district and town levels. By the results of task solutions possible losses of human and material resources as well as necessary forces and means for removal of these consequences are defined. (R.P.)

Radiation-induced genetic effects in germ cells of mammals

International Nuclear Information System (INIS)

Van Buul, P.P.W.

1993-01-01

The aim of the project is to gain information on the effects of ionizing radiation on germ cells of rodents and primates as measured by induced chromosomal translocations. Different aspects of the very significant interspecies differences between the mouse and the rhesus monkey (Macaca mulatta) for translocation induction in spermatogonial stem cells were studied. In addition, possible mechanisms for the well established reduced transmission of induced mouse translocations were investigated. (R.P.) 6 refs

Reusable Rapid Prototyped Blunt Impact Simulator

Science.gov (United States)

2016-08-01

of a few weeks instead of the months it would have taken to prototype and pursue a conventionally manufactured solution. 3. Future Work In the...NUMBER 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) Douglas A Petrick 5d. PROJECT NUMBER 5e. TASK NUMBER 5f. WORK UNIT NUMBER...impact area geometries were manufactured using selective laser sintering RP technology. These projectiles were launched with a compressed air cannon

Ubiquitin-activating enzyme UBA1 is required for cellular response to DNA damage

Czech Academy of Sciences Publication Activity Database

Moudrý, Pavel; Lukas, C.; Macůrek, Libor; Hanzlíková, Hana; Hodný, Zdeněk; Lukas, J.; Bartek, Jiří

2012-01-01

Roč. 11, č. 8 (2012), s. 1573-1582 ISSN 1538-4101 R&D Projects: GA ČR GA301/08/0353; GA ČR GAP301/10/1525 Grant - others:7.RP EU(XE) CZ.1.05/2.1.00/01.0030 Institutional research plan: CEZ:AV0Z50520514 Keywords : 53BP1 * DNA damage response * UBA1 * UBA6 * ubiquitylation Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 5.243, year: 2012

Project CHOICE: #67. A Career Unit for Grades 5 and 6. Introduction to Advertising. (Marketing and Distribution Occupations Career Cluster).

Science.gov (United States)

Kern County Superintendent of Schools, Bakersfield, CA.

This teaching unit, Introduction to Advertising, is one in a series of curriculum guides developed by Project CHOICE (Children Have Options in Career Education) to provide the classroom teacher with a source of career-related activities linking 5th and 6th grade elementary classroom experiences with the world of work. These ten lessons on…

EC6 design features and pre-project licensing review

Energy Technology Data Exchange (ETDEWEB)

Yu, S.; Lee, A.G.; Dinh, N.B.; Soulard, M. [CANDU Energy Inc., Mississauga, Ontario, (Canada)

2013-07-01

The Enhanced CANDU 6 (EC6) is the new Generation III CANDU reactor design that meets the most up to date Canadian regulatory requirements and customer expectations. Candu Energy Inc. is finalizing development of the EC6 which incorporates the CANDU 6's well-proven features, and adds enhancements that strengthened reactor safety margin and improved operability. The EC6 builds on the proven high performance design and the defence-in-depth features of CANDU 6 units, and has incorporated extensive operational feedback including lessons learned from Fukushima. This paper will provide status of the engineering program including progress on the pre-licensing review of the EC6 design by the Canadian Regulator, CNSC, and will also highlight the design and safety enhancements incorporated in the EC6 product. Safety enhancements to meet safety goals and to improve robustness of systems to respond to design basis accidents and beyond design basis accidents include: new severe accident recovery and heat removal system; improved emergency heat removal system; faster shutoff rods with improved safety margins; mechanical guaranteed shutdown rods; daily load cycling capability; robust containment with containment filter venting system; and improved backed-up electrical supply and cooling services. (author)

Increased projection of MHC and tumor antigens in murine B16-BL6 melanoma induced by hydrostatic pressure and chemical crosslinking.

Science.gov (United States)

Ramakrishna, V; Eisenthal, A; Skornick, Y; Shinitzky, M

1993-05-01

The B16-BL6 melanoma, like most spontaneously arising tumors, is poorly immunogenic and expresses low levels of major histocompatibility complex (MHC) antigens. Treatment of cells of this tumor in vitro by hydrostatic pressure in the presence of adenosine 2',3'-dialdehyde (oxAdo), a membrane-impermeant crosslinker, caused elevated projection of MHC and a specific tumor antigen as demonstrated by flow-cytometric analysis. Maximum projection of both the MHC and the tumor antigens could be reached by application of 1200 atm for 15 min in the presence of 20 mM oxAdo. It is not yet clear whether this passive increase in availability of antigens on the cell surface originated from a dormant pool of antigens in the plasma membrane or from pressure-induced fusion of antigen-rich intracellular organelles (e.g. the endoplasmic reticulum). The immunogenic properties of the antigen-enriched B16-BL6 cells are described in the following paper.

The Cyclohexanol Cycle and Synthesis of Nylon 6,6: Green Chemistry in the Undergraduate Organic Laboratory

Science.gov (United States)

Dintzner, Matthew R.; Kinzie, Charles R.; Pulkrabek, Kimberly; Arena, Anthony F.

2012-01-01

A one-term synthesis project that incorporates many of the principles of green chemistry is presented for the undergraduate organic laboratory. In this multistep scheme of reactions, students react, recycle, and ultimately convert cyclohexanol to nylon 6,6. The individual reactions in the project employ environmentally friendly methodologies, and…

Effect of parent-delivered action observation therapy on upper limb function in unilateral cerebral palsy: a randomized controlled trial.

Science.gov (United States)

Kirkpatrick, Emma; Pearse, Janice; James, Peter; Basu, Anna

2016-10-01

To determine whether home-based, parent-delivered therapy comprising action observation (AO) and repeated practice (RP) improves upper limb function more than RP alone in children with unilateral cerebral palsy (UCP). single-blinded parallel-group randomized controlled trial with 1:1 allocation comparing AO+RP (intervention) with RP alone (control). computer-generated, with allocation concealment by opaque sequentially-numbered envelopes. northern England, August 2011 to September 2013. 70 children with UCP; mean age 5.6 years (SD 2.1), 31 female. home-based activities were provided, tailored to interests and abilities. 15 minutes/day, 5 days/week for 3 months. Assisting Hand Assessment (AHA; primary outcome measure), Melbourne Assessment 2 (MA2), and ABILHAND-Kids at baseline, 3 months, and 6 months. Outcome data was available at 3 months for 28 children in the AO+RP group and 31 controls, and at 6 months for 26 and 28 children respectively. There were no between-group differences in AHA, MA2, or ABILHAND-Kids at 3 or 6 months versus baseline (all p>0.05). Combined-group improvements (pMA2 at 3 months, were maintained at 6 months. ABILHAND-Kids also showed improvement at 3 months (p=0.003), maintained at 6 months. Parent-delivered RP (with or without AO) improves upper limb function and could supplement therapist input. © 2016 The Authors. Developmental Medicine & Child Neurology published by John Wiley & Sons Ltd on behalf of Mac Keith Press.

Farm-Level Optimal Water Management : assistent for irrigation under deficit, second Executive summery report for FP6-European project nr. 036958

NARCIS (Netherlands)

Balendonck, J.

2008-01-01

FLOW-AID is a 6th Framework European project which started in autumn 2006. Its objective is to contribute to sustainability of irrigated agriculture by developing, testing in relevant conditions, and then optimizing an irrigation management system that can be used at farm level. The system will be

Instanton tunneling for de Sitter space with real projective spatial sections

Energy Technology Data Exchange (ETDEWEB)

Ong, Yen Chin [Center for Astronomy and Astrophysics, Department of Physics and Astronomy, Shanghai Jiao Tong University, Shanghai 200240 (China); Yeom, Dong-han, E-mail: ongyenchin@sjtu.edu.cn, E-mail: innocent.yeom@gmail.com [Leung Center for Cosmology and Particle Astrophysics, National Taiwan University, Taipei 10617, Taiwan (China)

2017-04-01

The physics of tunneling from one spacetime to another is often understood in terms of instantons. For some instantons, it was recently shown in the literature that there are two complementary ''interpretations'' for their analytic continuations. Dubbed ''something-to-something'' and ''nothing-to-something'' interpretations, respectively, the former involves situation in which the initial and final hypersurfaces are connected by a Euclidean manifold, whereas the initial and final hypersurfaces in the latter case are not connected in such a way. We consider a de Sitter space with real projective space RP{sup 3} spatial sections, as was originally understood by de Sitter himself. This original version of de Sitter space has several advantages over the usual de Sitter space with S{sup 3} spatial sections. In particular, the interpretation of the de Sitter entropy as entanglement entropy is much more natural. We discuss the subtleties involved in the tunneling of such a de Sitter space.

Development and validation of an RP-HPLC method for quantification of trans-resveratrol in the plant extracts

Directory of Open Access Journals (Sweden)

Cvetković Žika S.

2015-01-01

Full Text Available New, simple, cost effective, accurate and reproducible RP-HPLC method was developed and validated for the quantification of trans-resveratrol in the extracts of grape exocarp and seeds. The method has proved to be simpler and faster than available methods. Methanol was used as a mobile phase with a flow rate of 1.0 cm3 min-1, while the quantification was effected at 306 nm. The separation was performed at 35°C using a C18 column. The results showed that the peak area response was linear in the concentration range of 1-40 μg cm-3. The values of LOD and LOQ were found to be 0.125 and 0.413 μg cm-3, respectively. The antioxidant activity of the extracts was determined using DPPH assay. The ability of DPPH radicals inhibition decreases in the following order: the extract of grape exocarp > trans-resveratrol standard > the extract of grape seeds. [Projekat Ministarstva nauke Republike Srbije, br. TRp-34012

FY 1999 report on the results of the contract project 'The model project for facilities for effective utilization of industrial waste at the industrial complex in Thailand.' Separate Volume 6 - FY 1999 project; 1999 nendo seika hokokusho. Tai ni okeru kogyo danchi sangyo haikibutsu yuko riyo setsubi moderu jigyo - 6

Energy Technology Data Exchange (ETDEWEB)

NONE

2001-03-01

For the purpose of reducing the consumption of fossil fuel by recycling industrial waste for effective use as petroleum substituting energy in Thailand where the amount of industrial waste is expected to increase, a model project on facilities for effective use of industrial waste at the industrial complex was carried out, and the FY 1999 results were reported. Concretely, the industrial waste generated from each plant at the industrial complex owned by IEAT is to be incinerated in fluidized bed incinerator, and the process steam is to be generated by recovering waste heat by waste heat recovery boiler and to be supplied to plants within the complex. In this fiscal year, the first year of the project, the attachment to the agreement was prepared in terms of the allotment of the project work between Japan and Thailand, various kinds of gist, schedules, etc. and signed. After that, the following were conducted at the Japan side according to the attachment to the agreement: determination of the basic specifications for facilities, basic design, detailed design, manufacture of a part of the equipment, etc. Separate Volume 6 included drawings of assembling of the equipment such as crane, crusher and valve. (NEDO)

The "TEU-FEL" project