WorldWideScience

Sample records for project cmyc catch

  1. LCREP catch records - Lower Columbia River Ecosystem Monitoring Project

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — 1) The purpose of this project is to document juvenile salmon habitat occurrence in the Lower Columbia River and estuary, and examine how habitat conditions...

  2. ECLogger: Cross-Project Catch-Block Logging Prediction Using Ensemble of Classifiers

    Directory of Open Access Journals (Sweden)

    Sangeeta Lal

    2017-01-01

    Full Text Available Background: Software developers insert log statements in the source code to record program execution information. However, optimizing the number of log statements in the source code is challenging. Machine learning based within-project logging prediction tools, proposed in previous studies, may not be suitable for new or small software projects. For such software projects, we can use cross-project logging prediction. Aim: The aim of the study presented here is to investigate cross-project logging prediction methods and techniques. Method: The proposed method is ECLogger, which is a novel, ensemble-based, cross-project, catch-block logging prediction model. In the research We use 9 base classifiers were used and combined using ensemble techniques. The performance of ECLogger was evaluated on on three open-source Java projects: Tomcat, CloudStack and Hadoop. Results: ECLogger Bagging, ECLogger AverageVote, and ECLogger MajorityVote show a considerable improvement in the average Logged F-measure (LF on 3, 5, and 4 source -> target project pairs, respectively, compared to the baseline classifiers. ECLogger AverageVote performs best and shows improvements of 3.12% (average LF and 6.08% (average ACC – Accuracy. Conclusion: The classifier based on ensemble techniques, such as bagging, average vote, and majority vote outperforms the baseline classifier. Overall, the ECLogger AverageVote model performs best. The results show that the CloudStack project is more generalizable than the other projects.

  3. Promoting Energy-Balance Behaviors among Ethnically Diverse Adolescents: Overview and Baseline Findings of the Central Texas CATCH Middle School Project

    Science.gov (United States)

    Springer, Andrew E.; Kelder, Steven H.; Byrd-Williams, Courtney E.; Pasch, Keryn E.; Ranjit, Nalini; Delk, Joanne E.; Hoelscher, Deanna M.

    2013-01-01

    The Central Texas Coordinated Approach To Child Health (CATCH) Middle School Project is a 3.5-year school-based project aimed at promoting physical activity (PA), healthy eating, and obesity prevention among public middle school students in Texas. This article describes the CATCH intervention model and presents baseline findings from spring 2009.…

  4. Institutions of Catching-up Development (On the Project of a New Model for Economic Development of Russia

    Directory of Open Access Journals (Sweden)

    Viktor Meerovich Polterovich

    2016-11-01

    Full Text Available The article shows that institutional trajectories of catching-up development in successful countries including similar interim institutions; this similarity is explained by common technological, institutional and cultural limitations which need to be taken into account and overcome. Corporatism, indicative planning, the availability of the “general” development agency with broad mandate, undervalued exchange rate – these and some other mechanisms provide the countries of the “economic miracle” with an opportunity to initiate and maintain rapid economic growth despite the low level of human capital, underdeveloped civic culture and market failures. Institutions of catching-up development contributed to the formation of collaborative relations between the government, business and society. They eased the limitations and were gradually modified, providing a transition to modern democracies with efficient market economy. The conducted analysis helps outline a plan for an institutional reform taking into account Russia’s institutional features. The principles of administrative reform are considered. The author also sets the objective of forming a “hybrid” system of national planning which includes indicative planning and program budgeting. A combination of public-private partnership and program financing is proposed for the financing of the planned projects. The author proposes to use Japanese experience of promoting temporary association of companies for the development of modernization projects. The principles of reforming the systems of state property and science management are discussed

  5. Catch It If You Can: How Contagious Motivation Improves Group Projects and Course Satisfaction

    Science.gov (United States)

    Krishen, Anjala S.

    2013-01-01

    This article proposes a theory-based contagious motivation model focusing on enhancing student perceptions of group projects and ultimately course satisfaction. Moreover, drawing from both pedagogical and organizational behavior literatures, a model is presented that ties together intrinsic motivation theory with social contagion and…

  6. SIS - Annual Catch Limit

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Annual Catch Limit (ACL) dataset within the Species Information System (SIS) contains information and data related to management reference points and catch data.

  7. Evaluation of Molecular Inhibitors of the c-Myc Oncoprotein

    National Research Council Canada - National Science Library

    Prochownik, Edward V

    2005-01-01

    .... All of these functions require that C-Myc physically associate with another protein. Max. Example of diseases in which c-Myc deregulation occurs include breast cancer (approx. 30% of cases), colon cancer (>85...

  8. How to catch all those mutations--the report of the third Human Variome Project Meeting, UNESCO Paris, May 2010

    DEFF Research Database (Denmark)

    Kohonen-Corish, Maija R J; Al-Aama, Jumana Y; Auerbach, Arleen D

    2010-01-01

    framework of HVP activities: Ethics; Nomenclature and Standards; Publication, Credit and Incentives; Data Collection from Clinics; Overall Data Integration and Access-Peripheral Systems/Software; Data Collection from Laboratories; Assessment of Pathogenicity; Country Specific Collection; Translation...... disease-specific variation data collection and utilization: the International Society for Gastrointestinal Hereditary Tumours, the Micronutrient Genomics Project, and the Neurogenetics Consortium....

  9. How to Catch All Those Mutations—The Report of the Third Human Variome Project Meeting, UNESCO Paris, May 2010

    Science.gov (United States)

    Kohonen-Corish, Maija R.J.; Al-Aama, Jumana Y.; Auerbach, Arleen D.; Axton, Myles; Barash, Carol Isaacson; Bernstein, Inge; Béroud, Christophe; Burn, John; Cunningham, Fiona; Cutting, Garry R.; den Dunnen, Johan T.; Greenblatt, Marc S.; Kaput, Jim; Katz, Michael; Lindblom, Annika; Macrae, Finlay; Maglott, Donna; Möslein, Gabriela; Povey, Sue; Ramesar, Raj; Richards, Sue; Seminara, Daniela; Sobrido, María-Jesús; Tavtigian, Sean; Taylor, Graham; Vihinen, Mauno; Winship, Ingrid; Cotton, Richard G.H.

    2011-01-01

    The third Human Variome Project (HVP) Meeting “Integration and Implementation” was held under UNESCO Patronage in Paris, France, at the UNESCO Headquarters May 10–14, 2010. The major aims of the HVP are the collection, curation, and distribution of all human genetic variation affecting health. The HVP has drawn together disparate groups, by country, gene of interest, and expertise, who are working for the common good with the shared goal of pushing the boundaries of the human variome and collaborating to avoid unnecessary duplication. The meeting addressed the 12 key areas that form the current framework of HVP activities: Ethics; Nomenclature and Standards; Publication, Credit and Incentives; Data Collection from Clinics; Overall Data Integration and Access—Peripheral Systems/Software; Data Collection from Laboratories; Assessment of Pathogenicity; Country Specific Collection; Translation to Healthcare and Personalized Medicine; Data Transfer, Databasing, and Curation; Overall Data Integration and Access—Central Systems; and Funding Mechanisms and Sustainability. In addition, three societies that support the goals and the mission of HVP also held their own Workshops with the view to advance disease-specific variation data collection and utilization: the International Society for Gastrointestinal Hereditary Tumours, the Micronutrient Genomics Project, and the Neurogenetics Consortium. PMID:20960468

  10. How to catch all those mutations--the report of the third Human Variome Project Meeting, UNESCO Paris, May 2010.

    Science.gov (United States)

    Kohonen-Corish, Maija R J; Al-Aama, Jumana Y; Auerbach, Arleen D; Axton, Myles; Barash, Carol Isaacson; Bernstein, Inge; Béroud, Christophe; Burn, John; Cunningham, Fiona; Cutting, Garry R; den Dunnen, Johan T; Greenblatt, Marc S; Kaput, Jim; Katz, Michael; Lindblom, Annika; Macrae, Finlay; Maglott, Donna; Möslein, Gabriela; Povey, Sue; Ramesar, Raj; Richards, Sue; Seminara, Daniela; Sobrido, María-Jesús; Tavtigian, Sean; Taylor, Graham; Vihinen, Mauno; Winship, Ingrid; Cotton, Richard G H

    2010-12-01

    The third Human Variome Project (HVP) Meeting "Integration and Implementation" was held under UNESCO Patronage in Paris, France, at the UNESCO Headquarters May 10-14, 2010. The major aims of the HVP are the collection, curation, and distribution of all human genetic variation affecting health. The HVP has drawn together disparate groups, by country, gene of interest, and expertise, who are working for the common good with the shared goal of pushing the boundaries of the human variome and collaborating to avoid unnecessary duplication. The meeting addressed the 12 key areas that form the current framework of HVP activities: Ethics; Nomenclature and Standards; Publication, Credit and Incentives; Data Collection from Clinics; Overall Data Integration and Access-Peripheral Systems/Software; Data Collection from Laboratories; Assessment of Pathogenicity; Country Specific Collection; Translation to Healthcare and Personalized Medicine; Data Transfer, Databasing, and Curation; Overall Data Integration and Access-Central Systems; and Funding Mechanisms and Sustainability. In addition, three societies that support the goals and the mission of HVP also held their own Workshops with the view to advance disease-specific variation data collection and utilization: the International Society for Gastrointestinal Hereditary Tumours, the Micronutrient Genomics Project, and the Neurogenetics Consortium. © 2010 Wiley-Liss, Inc.

  11. Mapping Lake Michigan Fish Catch Data

    OpenAIRE

    Wodd, Jacob; Doucette, Jarrod; Höök, Tomas O.

    2014-01-01

    The only Great Lake completely contained in the U.S., Lake Michigan offers an abundance of recreational fishing. This project takes 20 years’ worth of salmonid fish catch data, and uses GIS to organize and visually represent the data in a way that is meaningful and helpful to local fisherman and researchers. Species represented included Brown Trout, Lake Trout, Rainbow Trout, Chinook Salmon, and Coho Salmon. The species are organized by both decadal and yearly spans, as well as catch per t...

  12. Catch-In-Areas Main

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Catch-In-Areas database integrates catch data from the Catch Accounting System (which has the spatial resolution of a NMFS Reporting Area) into a database that...

  13. Fluorescent Dansyl-Guanosine Conjugates that Bind c-MYC Promoter G-Quadruplex and Downregulate c-MYC Expression.

    Science.gov (United States)

    Pavan Kumar, Y; Saha, Puja; Saha, Dhurjhoti; Bessi, Irene; Schwalbe, Harald; Chowdhury, Shantanu; Dash, Jyotirmayee

    2016-03-02

    The four-stranded G-quadruplex present in the c-MYC P1 promoter has been shown to play a pivotal role in the regulation of c-MYC transcription. Small-molecule compounds capable of inhibiting the c-MYC promoter activity by stabilising the c-MYC G-quadruplex could potentially be used as anticancer agents. In this context, here we report the synthesis of dansyl-guanosine conjugates through one-pot modular click reactions. The dansyl-guanosine conjugates can selectively detect c-MYC G-quadruplex over other biologically relevant quadruplexes and duplex DNA and can be useful as staining reagents for selective visualisation of c-MYC G-quadruplex over duplex DNA by gel electrophoresis. NMR spectroscopic titrations revealed the preferential binding sites of these dansyl ligands to the c-MYC G-quadruplex. A dual luciferase assay and qRT-PCR revealed that a dansyl-bisguanosine ligand represses the c-MYC expression, possibly by stabilising the c-MYC G-quadruplex. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  14. c-myc overexpression causes anaplasia in medulloblastoma.

    Science.gov (United States)

    Stearns, Duncan; Chaudhry, Aneeka; Abel, Ty W; Burger, Peter C; Dang, Chi V; Eberhart, Charles G

    2006-01-15

    Both anaplasia and increased c-myc gene expression have been shown to be negative prognostic indicators for survival in medulloblastoma patients. myc gene amplification has been identified in many large cell/anaplastic medulloblastoma, but no causative link between c-myc and anaplastic changes has been established. To address this, we stably overexpressed c-myc in two medulloblastoma cell lines, DAOY and UW228, and examined the changes in growth characteristics. When analyzed in vitro, cell lines with increased levels of c-myc had higher rates of growth and apoptosis as well as significantly improved ability to form colonies in soft agar compared with control. When injected s.c. into nu/nu mice, flank xenograft tumors with high levels of c-myc in DAOY cell line background were 75% larger than those derived from control. Overexpression of c-myc was required for tumor formation by UW228 cells. Most remarkably, the histopathology of the Myc tumors was severely anaplastic, with large areas of necrosis/apoptosis, increased nuclear size, and macronucleoli. Indices of proliferation and apoptosis were also significantly higher in Myc xenografts. Thus, c-myc seems to play a causal role in inducing anaplasia in medulloblastoma. Because anaplastic changes are often observed in recurrent medulloblastoma, we propose that c-myc dysregulation is involved in the progression of these malignant embryonal neoplasms.

  15. School students "Catch a Star"!

    Science.gov (United States)

    2007-04-01

    School students from across Europe and beyond have won prizes in an astronomy competition, including the trip of a lifetime to one of the world's most powerful astronomical observatories, on a mountaintop in Chile. ESO, the European Organisation for Astronomical Research in the Southern Hemisphere, together with the European Association for Astronomy Education (EAAE), has just announced the winners of the 2007 "Catch a Star!" competition. ESO PR Photo 21/07 "Catch a Star!" is an international astronomy competition for school students, in which students are invited to 'become astronomers' and explore the Universe. The competition includes two categories for written projects on astronomical themes, to ensure that every student, whatever their level, has the chance to enter and win exciting prizes. For the artistically minded, "Catch a Star!" also includes an astronomy-themed artwork competition. Students from 22 countries submitted hundreds of written projects and pieces of artwork. "The standard of entries was most impressive, and made the jury's task of choosing winners both enjoyable and difficult! We hope that everyone, whether or not they won a prize, had fun taking part, and learnt some exciting things about our Universe", said Douglas Pierce-Price, Education Officer at ESO. The top prize, of a week-long trip to Chile to visit the ESO Very Large Telescope (VLT) on Paranal, was won by students Jan Mestan and Jan Kotek from Gymnazium Pisek in the Czech Republic, together with their teacher Marek Tyle. Their report on "Research and Observation of the Solar Eclipse" told how they had studied solar eclipses, and involved their fellow students in observations of an eclipse from their school in 2006. The team will travel to Chile and visit the ESO VLT - one of the world's most powerful optical/infrared telescopes - where they will meet astronomers and be present during a night of observations on the 2600m high Paranal mountaintop. "It's fantastic that we will see the

  16. Postnatal liver growth and regeneration are independent of c-myc in a mouse model of conditional hepatic c-myc deletion

    Directory of Open Access Journals (Sweden)

    Sanders Jennifer A

    2012-03-01

    Full Text Available Abstract Background The transcription factor c-myc regulates genes involved in hepatocyte growth, proliferation, metabolism, and differentiation. It has also been assigned roles in liver development and regeneration. In previous studies, we made the unexpected observation that c-Myc protein levels were similar in proliferating fetal liver and quiescent adult liver with c-Myc displaying nucleolar localization in the latter. In order to investigate the functional role of c-Myc in adult liver, we have developed a hepatocyte-specific c-myc knockout mouse, c-mycfl/fl;Alb-Cre. Results Liver weight to body weight ratios were similar in control and c-myc deficient mice. Liver architecture was unaffected. Conditional c-myc deletion did not result in compensatory induction of other myc family members or in c-Myc's binding partner Max. Floxed c-myc did have a negative effect on Alb-Cre expression at 4 weeks of age. To explore this relationship further, we used the Rosa26 reporter line to assay Cre activity in the c-myc floxed mice. No significant difference in Alb-Cre activity was found between control and c-mycfl/fl mice. c-myc deficient mice were studied in a nonproliferative model of liver growth, fasting for 48 hr followed by a 24 hr refeeding period. Fasting resulted in a decrease in liver mass and liver protein, both of which recovered upon 24 h of refeeding in the c-mycfl/fl;Alb-Cre animals. There was also no effect of reducing c-myc on recovery of liver mass following 2/3 partial hepatectomy. Conclusions c-Myc appears to be dispensable for normal liver growth during the postnatal period, restoration of liver mass following partial hepatectomy and recovery from fasting.

  17. Ezrin mediates c-Myc actions in prostate cancer cell invasion

    DEFF Research Database (Denmark)

    Chuan, Yin Choy; Iglesias Gato, Diego; Fernandez-Perez, L

    2010-01-01

    The forced overexpression of c-Myc in mouse prostate and in normal human prostate epithelial cells results in tumor transformation with an invasive phenotype. How c-Myc regulates cell invasion is poorly understood. In this study, we have investigated the interplay of c-Myc and androgens in the re...

  18. Hawaii DAR Fisherman Reporting System Data (Catch)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains the catch data from fishers who take marine life for commercial purposes and report their catch, effort, and sales on a commercial catch...

  19. Shikonin regulates C-MYC and GLUT1 expression through the MST1-YAP1-TEAD1 axis

    Czech Academy of Sciences Publication Activity Database

    Vališ, Karel; Talacko, Pavel; Grobárová, Valeria; Černý, J.; Novák, Petr

    2016-01-01

    Roč. 349, č. 2 (2016), s. 273-281 ISSN 0014-4827 R&D Projects: GA ČR(CZ) GP14-21095P; GA ČR GA13-16565S; GA MŠk(CZ) LQ1604; GA MŠk(CZ) ED1.1.00/02.0109 Institutional support: RVO:61388971 Keywords : Hippo * Glycolysis * C-MYC Subject RIV: EE - Microbiology, Virology Impact factor: 3.546, year: 2016

  20. Endogenous c-Myc is essential for p53-induced apoptosis in response to DNA damage in vivo

    NARCIS (Netherlands)

    Phesse, T. J.; Myant, K. B.; Cole, A. M.; Ridgway, R. A.; Pearson, H.; Muncan, V.; van den Brink, G. R.; Vousden, K. H.; Sears, R.; Vassilev, L. T.; Clarke, A. R.; Sansom, O. J.

    2014-01-01

    Recent studies have suggested that C-MYC may be an excellent therapeutic cancer target and a number of new agents targeting C-MYC are in preclinical development. Given most therapeutic regimes would combine C-MYC inhibition with genotoxic damage, it is important to assess the importance of C-MYC

  1. LPS Catch and Effort Estimation

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data collected from the LPS dockside (LPIS) and the LPS telephone (LPTS) surveys are combined to produce estimates of total recreational catch, landings, and fishing...

  2. BIOGAS PRODUCTION FROM CATCH CROPS

    DEFF Research Database (Denmark)

    Molinuevo-Salces, Beatriz; Larsen, Søren U.; Ahring, Birgitte Kiær

    2014-01-01

    -substrate in manure-based biogas plants and the profit obtained from the sale of biogas barely compensates for the harvest costs. A new agricultural strategy to harvest catch crops together with the residual straw of the main crop was investigated to increase the biomass and thereby the methane yield per hectare......Catch crop cultivation combined with its use for biogas production would increase renewable energy production in the form of methane, without interfering with the production of food and fodder crops. The low biomass yield of catch crops is the main limiting factor for using these crops as co...... biomass. Leaving the straw on the field until harvest of the catch crop in the autumn could benefit biogas production due to the organic matter degradation of the straw taking place on the field during the autumn months. This new agricultural strategy may be a good alternative to achieve economically...

  3. c-MYC amplification and c-myc protein expression in pancreatic acinar cell carcinomas. New insights into the molecular signature of these rare cancers.

    Science.gov (United States)

    La Rosa, Stefano; Bernasconi, Barbara; Vanoli, Alessandro; Sciarra, Amedeo; Notohara, Kenji; Albarello, Luca; Casnedi, Selenia; Billo, Paola; Zhang, Lizhi; Tibiletti, Maria Grazia; Sessa, Fausto

    2018-05-02

    The molecular alterations of pancreatic acinar cell carcinomas (ACCs) and mixed acinar-neuroendocrine carcinomas (MANECs) are not completely understood, and the possible role of c-MYC amplification in tumor development, progression, and prognosis is not known. We have investigated c-MYC gene amplification in a series of 35 ACCs and 4 MANECs to evaluate its frequency and a possible prognostic role. Gene amplification was investigated using interphasic fluorescence in situ hybridization analysis simultaneously hybridizing c-MYC and the centromere of chromosome 8 probes. Protein expression was immunohistochemically investigated using a specific monoclonal anti-c-myc antibody. Twenty cases had clones with different polysomies of chromosome 8 in absence of c-MYC amplification, and 5 cases had one amplified clone and other clones with chromosome 8 polysomy, while the remaining 14 cases were diploid for chromosome 8 and lacked c-MYC amplification. All MANECs showed c-MYC amplification and/or polysomy which were observed in 54% pure ACCs. Six cases (15.3%) showed nuclear immunoreactivity for c-myc, but only 4/39 cases showed simultaneous c-MYC amplification/polysomy and nuclear protein expression. c-myc immunoreactivity as well as c-MYC amplification and/or chromosome 8 polysomy was not statistically associated with prognosis. Our study demonstrates that a subset of ACCs shows c-MYC alterations including gene amplification and chromosome 8 polysomy. Although they are not associated with a different prognostic signature, the fact that these alterations are present in all MANECs suggests a role in the acinar-neuroendocrine differentiation possibly involved in the pathogenesis of MANECs.

  4. Mechanisms of c-myc degradation by nickel compounds and hypoxia.

    Directory of Open Access Journals (Sweden)

    Qin Li

    2009-12-01

    Full Text Available Nickel (Ni compounds have been found to cause cancer in humans and animal models and to transform cells in culture. At least part of this effect is mediated by stabilization of hypoxia inducible factor (HIF1a and activating its downstream signaling. Recent studies reported that hypoxia signaling might either antagonize or enhance c-myc activity depending on cell context. We investigated the effect of nickel on c-myc levels, and demonstrated that nickel, hypoxia, and other hypoxia mimetics degraded c-myc protein in a number of cancer cells (A549, MCF-7, MDA-453, and BT-474. The degradation of the c-Myc protein was mediated by the 26S proteosome. Interestingly, knockdown of both HIF-1alpha and HIF-2alpha attenuated c-Myc degradation induced by Nickel and hypoxia, suggesting the functional HIF-1alpha and HIF-2alpha was required for c-myc degradation. Further studies revealed two potential pathways mediated nickel and hypoxia induced c-myc degradation. Phosphorylation of c-myc at T58 was significantly increased in cells exposed to nickel or hypoxia, leading to increased ubiquitination through Fbw7 ubiquitin ligase. In addition, nickel and hypoxia exposure decreased USP28, a c-myc de-ubiquitinating enzyme, contributing to a higher steady state level of c-myc ubiquitination and promoting c-myc degradation. Furthermore, the reduction of USP28 protein by hypoxia signaling is due to both protein degradation and transcriptional repression. Nickel and hypoxia exposure significantly increased the levels of dimethylated H3 lysine 9 at the USP28 promoter and repressed its expression. Our study demonstrated that Nickel and hypoxia exposure increased c-myc T58 phosphorylation and decreased USP28 protein levels in cancer cells, which both lead to enhanced c-myc ubiquitination and proteasomal degradation.

  5. Mechanism of estrogen activation of c-myc oncogene expression.

    Science.gov (United States)

    Dubik, D; Shiu, R P

    1992-08-01

    The estrogen receptor complex is a known trans-acting factor that regulates transcription of specific genes through an interaction with a specific estrogen-responsive cis-acting element (ERE). In previous studies we have shown that in estrogen-responsive human breast cancer cells estrogen rapidly activates c-myc expression. This activated expression occurs through enhanced transcription and does not require the synthesis of new protein intermediates; therefore, an ERE is present in the human c-myc gene regulatory region. To localize the ERE, constructs containing varying lengths of the c-myc 5'-flanking region ranging from -2327 to +25 (relative to the P1 promoter) placed adjacent to the chloramphenicol acetyl transferase reporter gene (CAT) were prepared. They were used in transient transfection studies in MCF-7 and HeLa cells co-transfected with an estrogen receptor expression vector. These studies reveal that all constructs containing the P2 promoter region exhibited estrogen-regulated CAT expression and that a 116-bp region upstream and encompassing the P2 TATA box is necessary for this activity. Analysis of this 116-bp region failed to identify a cis-acting element with sequences resembling the consensus ERE; however, co-transfection studies with mutant estrogen receptor expression vectors showed that the DNA-binding domain of the receptor is essential for estrogen-regulated CAT gene expression. We have also observed that anti-estrogen receptor complexes can weakly trans-activate from this 116-bp region but fail to do so from the ERE-containing ApoVLDLII-CAT construct. To explain these results we propose a new mechanism of estrogen trans-activation in the c-myc gene promoter.

  6. Rictor regulates FBXW7-dependent c-Myc and cyclin E degradation in colorectal cancer cells

    Energy Technology Data Exchange (ETDEWEB)

    Guo, Zheng [Markey Cancer Center, The University of Kentucky, 800 Rose Street, Lexington, KY 40536 (United States); Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, 1838 Guangzhou Dadao Bei, Guangzhou 510515 (China); Zhou, Yuning [Markey Cancer Center, The University of Kentucky, 800 Rose Street, Lexington, KY 40536 (United States); Evers, B. Mark [Markey Cancer Center, The University of Kentucky, 800 Rose Street, Lexington, KY 40536 (United States); Department of Surgery, The University of Kentucky, 800 Rose Street, Lexington, KY 40536 (United States); Wang, Qingding, E-mail: qingding.wang@uky.edu [Markey Cancer Center, The University of Kentucky, 800 Rose Street, Lexington, KY 40536 (United States); Department of Surgery, The University of Kentucky, 800 Rose Street, Lexington, KY 40536 (United States)

    2012-02-10

    Highlights: Black-Right-Pointing-Pointer Rictor associates with FBXW7 to form an E3 complex. Black-Right-Pointing-Pointer Knockdown of rictor decreases ubiquitination of c-Myc and cylin E. Black-Right-Pointing-Pointer Knockdown of rictor increases protein levels of c-Myc and cylin E. Black-Right-Pointing-Pointer Overexpression of rictor induces the degradation of c-Myc and cyclin E proteins. Black-Right-Pointing-Pointer Rictor regulation of c-Myc and cyclin E requires FBXW7. -- Abstract: Rictor (Rapamycin-insensitive companion of mTOR) forms a complex with mTOR and phosphorylates and activates Akt. Activation of Akt induces expression of c-Myc and cyclin E, which are overexpressed in colorectal cancer and play an important role in colorectal cancer cell proliferation. Here, we show that rictor associates with FBXW7 to form an E3 complex participating in the regulation of c-Myc and cyclin E degradation. The Rictor-FBXW7 complex is biochemically distinct from the previously reported mTORC2 and can be immunoprecipitated independently of mTORC2. Moreover, knocking down of rictor in serum-deprived colorectal cancer cells results in the decreased ubiquitination and increased protein levels of c-Myc and cyclin E while overexpression of rictor induces the degradation of c-Myc and cyclin E proteins. Genetic knockout of FBXW7 blunts the effects of rictor, suggesting that rictor regulation of c-Myc and cyclin E requires FBXW7. Our findings identify rictor as an important component of FBXW7 E3 ligase complex participating in the regulation of c-Myc and cyclin E protein ubiquitination and degradation. Importantly, our results suggest that elevated growth factor signaling may contribute to decrease rictor/FBXW7-mediated ubiquitination of c-Myc and cyclin E, thus leading to accumulation of cyclin E and c-Myc in colorectal cancer cells.

  7. Rictor regulates FBXW7-dependent c-Myc and cyclin E degradation in colorectal cancer cells

    International Nuclear Information System (INIS)

    Guo, Zheng; Zhou, Yuning; Evers, B. Mark; Wang, Qingding

    2012-01-01

    Highlights: ► Rictor associates with FBXW7 to form an E3 complex. ► Knockdown of rictor decreases ubiquitination of c-Myc and cylin E. ► Knockdown of rictor increases protein levels of c-Myc and cylin E. ► Overexpression of rictor induces the degradation of c-Myc and cyclin E proteins. ► Rictor regulation of c-Myc and cyclin E requires FBXW7. -- Abstract: Rictor (Rapamycin-insensitive companion of mTOR) forms a complex with mTOR and phosphorylates and activates Akt. Activation of Akt induces expression of c-Myc and cyclin E, which are overexpressed in colorectal cancer and play an important role in colorectal cancer cell proliferation. Here, we show that rictor associates with FBXW7 to form an E3 complex participating in the regulation of c-Myc and cyclin E degradation. The Rictor–FBXW7 complex is biochemically distinct from the previously reported mTORC2 and can be immunoprecipitated independently of mTORC2. Moreover, knocking down of rictor in serum-deprived colorectal cancer cells results in the decreased ubiquitination and increased protein levels of c-Myc and cyclin E while overexpression of rictor induces the degradation of c-Myc and cyclin E proteins. Genetic knockout of FBXW7 blunts the effects of rictor, suggesting that rictor regulation of c-Myc and cyclin E requires FBXW7. Our findings identify rictor as an important component of FBXW7 E3 ligase complex participating in the regulation of c-Myc and cyclin E protein ubiquitination and degradation. Importantly, our results suggest that elevated growth factor signaling may contribute to decrease rictor/FBXW7-mediated ubiquitination of c-Myc and cyclin E, thus leading to accumulation of cyclin E and c-Myc in colorectal cancer cells.

  8. Catching up with the Keynesians

    NARCIS (Netherlands)

    Ljungqvist, L.; Uhlig, H.F.H.V.S.

    1996-01-01

    This paper examines the role for tax policies in productivity-shock driven economies with \\catching-up-with-the-Joneses" utility functions.The optimal tax policy is shown to a ect the economy countercyclically via procyclical taxes, i.e., \\cooling down" the economy with higher taxes when it is

  9. MSY from catch and resilience

    DEFF Research Database (Denmark)

    Jørgensen, Ole A; Chrysafi, Anna

    A simple Schaefer model was tested on the Greenland halibut stock offshore in NAFO SA 0 and 1. The minimum data required for this model is a catch time series and a measure of the resilience of the species. Other input parameters that had to be guessed were the carrying capacity, the biomass...

  10. WHEN SCIENCE CATCHES THE EYE

    Indian Academy of Sciences (India)

    WHEN SCIENCE CATCHES THE EYE · Slide 2 · Slide 3 · Slide 4 · Concept Overview · Slide 6 · Slide 7 · Slide 8 · Accommodation: Biomechanics · Accommodative IOL: Crystalens · Slide 11 · Enhanced Single Optic Designs · A Dual Optic Accommodating IOL · Slide 14 · A-IOL Implantation being done by Dr. Virender ...

  11. Passing and Catching in Rugby.

    Science.gov (United States)

    Namudu, Mike M.

    This booklet contains the fundamentals for rugby at the primary school level. It deals primarily with passing and catching the ball. It contains instructions on (1) holding the ball for passing, (2) passing the ball to the left--standing, (3) passing the ball to the left--running, (4) making a switch pass, (5) the scrum half's normal pass, (6) the…

  12. Catch-up operation on old pesticides: an integration

    NARCIS (Netherlands)

    Canton JH; Linders JBHJ; Luttik R; Mensink BJWG; Panman E; Plassche EJ van de; Sparenburg PM; Tuinstra J

    1991-01-01

    The "catch-up operation on old pesticides" project has resulted in a summary and RIVM conclusion of the environmental aspects of 152 pesticides which were already marketed in the Netherlands before 1975. The RIVM conclusion was subsequently rewritten to form an environmental synopsis. The

  13. Catch-up operation on old pesticides: an integration

    NARCIS (Netherlands)

    Canton JH; Linders JBHJ; Luttik R; Mensink BJWG; Panman E; van de Plassche EJ; Sparenburg PM; Tuinstra J

    1991-01-01

    The "catch-up operation on old pesticides" project has resulted in a summary and RIVM conclusion of the environmental aspects of 152 pesticides which were already marketed in the Netherlands before 1975. The RIVM conclusion was subsequently rewritten to form an environmental synopsis. The present

  14. AKRO/SF: Catch Accounting System (CAS)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Catch Accounting System (CAS) creates total catch estimates for the groundfish fisheries in the Bering Sea/Aleutian Islands and Gulf of Alaska. Each year, quotas...

  15. Clinicopathological significance of c-MYC in esophageal squamous cell carcinoma.

    Science.gov (United States)

    Lian, Yu; Niu, Xiangdong; Cai, Hui; Yang, Xiaojun; Ma, Haizhong; Ma, Shixun; Zhang, Yupeng; Chen, Yifeng

    2017-07-01

    Esophageal squamous cell carcinoma is one of the most common malignant tumors. The oncogene c-MYC is thought to be important in the initiation, promotion, and therapy resistance of cancer. In this study, we aim to investigate the clinicopathologic roles of c-MYC in esophageal squamous cell carcinoma tissue. This study is aimed at discovering and analyzing c-MYC expression in a series of human esophageal tissues. A total of 95 esophageal squamous cell carcinoma samples were analyzed by the western blotting and immunohistochemistry techniques. Then, correlation of c-MYC expression with clinicopathological features of esophageal squamous cell carcinoma patients was statistically analyzed. In most esophageal squamous cell carcinoma cases, the c-MYC expression was positive in tumor tissues. The positive rate of c-MYC expression in tumor tissues was 61.05%, obviously higher than the adjacent normal tissues (8.42%, 8/92) and atypical hyperplasia tissues (19.75%, 16/95). There was a statistical difference among adjacent normal tissues, atypical hyperplasia tissues, and tumor tissues. Overexpression of the c-MYC was detected in 61.05% (58/95) esophageal squamous cell carcinomas, which was significantly correlated with the degree of differentiation (p = 0.004). The positive rate of c-MYC expression was 40.0% in well-differentiated esophageal tissues, with a significantly statistical difference (p = 0.004). The positive rate of c-MYC was 41.5% in T1 + T2 esophageal tissues and 74.1% in T3 + T4 esophageal tissues, with a significantly statistical difference (p = 0.001). The positive rate of c-MYC was 45.0% in I + II esophageal tissues and 72.2% in III + IV esophageal tissues, with a significantly statistical difference (p = 0.011). The c-MYC expression strongly correlated with clinical staging (p = 0.011), differentiation degree (p = 0.004), lymph node metastasis (p = 0.003), and invasion depth (p = 0.001) of patients with esophageal squamous cell carcinoma. The c-MYC was

  16. Biogas production from catch crops

    DEFF Research Database (Denmark)

    Molinuevo-Salces, Beatriz; Larsen, Søren U.; Ahring, Birgitte Kiær

    2013-01-01

    , being in the ranges of 1.4–3.0 t ha−1 and 0.3–1.7 t ha−1 for Holstebro and Aabenraa, respectively. Specific methane yields were in the range of 229–450 m3 t−1 of VS. Methane yields per hectare of up to 800 m3 ha−1 were obtained, making catch crops a promising source of feedstock for manure-based biogas......Manure-based biogas plants in Denmark are dependent on high yielding biomass feedstock in order to secure economically feasible operation. The aim of this study was to investigate the potential of ten different catch crop species or mixtures as feedstock for biogas production in co...

  17. Driver or passenger effects of augmented c-Myc and Cdc20 in gliomagenesis.

    Science.gov (United States)

    Ji, Ping; Zhou, Xinhui; Liu, Qun; Fuller, Gregory N; Phillips, Lynette M; Zhang, Wei

    2016-04-26

    Cdc20 and c-Myc are commonly overexpressed in a broad spectrum of cancers, including glioblastoma (GBM). Despite this clear association, whether c-Myc and Cdc20 overexpression is a driver or passenger event in gliomagenesis remains unclear. Both c-Myc and Cdc20 induced the proliferation of primary glial progenitor cells. c-Myc also promoted the formation of soft agar anchorage-independent colonies. In the RCAS/Ntv-a glia-specific transgenic mouse model, c-Myc increased the GBM incidence from 19.1% to 47.4% by 12 weeks of age when combined with kRas and Akt3 in Ntv-a INK4a-ARF (also known as CDKN2A)-null mice. In contrast, Cdc20 decreased the GBM incidence from 19.1% to 9.1%. Moreover, cell differentiation was modulated by c-Myc in kRas/Akt3-induced GBM on the basis of Nestin/GFAP expression (glial progenitor cell differentiation), while Cdc20 had no effect on primary glial progenitor cell differentiation. We used glial progenitor cells from Ntv-a newborn mice to evaluate the role of c-Myc and Cdc20 in the proliferation and transformation of GBM in vitro and in vivo. We further determined whether c-Myc and Cdc20 have a driver or passenger role in GBM development using kRas/Akt3 signals in a RCAS/Ntv-a mouse model. These results suggest that the driver or passenger of oncogene signaling is dependent on cellular status. c-Myc is a driver when combined with kRas/Akt3 oncogenic signals in gliomagenesis, whereas Cdc20 overexpression is a passenger. Inhibition of cell differentiation of c-Myc may be a target for anti-glioma therapy.

  18. c-Myc activates BRCA1 gene expression through distal promoter elements in breast cancer cells

    International Nuclear Information System (INIS)

    Chen, Yinghua; Xu, Jinhua; Borowicz, Stanley; Collins, Cindy; Huo, Dezheng; Olopade, Olufunmilayo I

    2011-01-01

    The BRCA1 gene plays an important role in the maintenance of genomic stability. BRCA1 inactivation contributes to breast cancer tumorigenesis. An increasing number of transcription factors have been shown to regulate BRCA1 expression. c-Myc can act as a transcriptional activator, regulating up to 15% of all genes in the human genome and results from a high throughput screen suggest that BRCA1 is one of its targets. In this report, we used cultured breast cancer cells to examine the mechanisms of transcriptional activation of BRCA1 by c-Myc. c-Myc was depleted using c-Myc-specific siRNAs in cultured breast cancer cells. BRCA1 mRNA expression and BRCA1 protein expression were determined by quantitative RT-PCR and western blot, respectively and BRCA1 promoter activities were examined under these conditions. DNA sequence analysis was conducted to search for high similarity to E boxes in the BRCA1 promoter region. The association of c-Myc with the BRCA1 promoter in vivo was tested by a chromatin immunoprecipitation assay. We investigated the function of the c-Myc binding site in the BRCA1 promoter region by a promoter assay with nucleotide substitutions in the putative E boxes. BRCA1-dependent DNA repair activities were measured by a GFP-reporter assay. Depletion of c-Myc was found to be correlated with reduced expression levels of BRCA1 mRNA and BRCA1 protein. Depletion of c-Myc decreased BRCA1 promoter activity, while ectopically expressed c-Myc increased BRCA1 promoter activity. In the distal BRCA1 promoter, DNA sequence analysis revealed two tandem clusters with high similarity, and each cluster contained a possible c-Myc binding site. c-Myc bound to these regions in vivo. Nucleotide substitutions in the c-Myc binding sites in these regions abrogated c-Myc-dependent promoter activation. Furthermore, breast cancer cells with reduced BRCA1 expression due to depletion of c-Myc exhibited impaired DNA repair activity. The distal BRCA1 promoter region is associated with c-Myc

  19. AP-2α Inhibits c-MYC Induced Oxidative Stress and Apoptosis in HaCaT Human Keratinocytes

    Directory of Open Access Journals (Sweden)

    Lei Yu

    2009-01-01

    AP-2 may have a direct effect on the c-myc gene. Chromatin immunoprecipitation assays demonstrated that AP-2 proteins bound to a cluster of AP-2 binding sites located within a 2 kb upstream regulatory region of c-myc These results suggest that the negative regulation of AP-2 on c-MYC activity was achieved through binding of AP-2 protein to the c-myc gene. The effects of AP-2 on c-MYC induced ROS accumulation and apoptosis in epidermal keratinocytes are likely to play an important role in cell growth, differentiation and carcinogenesis of the skin.

  20. c-myc Amplification Is Frequent in Esophageal Adenocarcinoma and Correlated with the Upregulation of VEGF-A Expression

    Directory of Open Access Journals (Sweden)

    Burkhard H.A. von Rahden

    2006-09-01

    Full Text Available BACKGROUND: Deregulation of c-myc plays a major role in the carcinogenesis of human malignancies. We investigated the amplification of the c-myc gene in a surgical series of Barrett cancers. METHODS: Primary resected esophageal (Barrett adenocarcinomas (n = 84 were investigated for c-myc amplification using chromogene in situ hybridization. Tumor samples were assembled in a tissue microarray. c-myc gene dosage was correlated with clinicopathologic parameters, including the survival and gene expression of cyclooxygenases (COX-1 and COX-2 and proangiogenic growth factors (VEGF-A and VEGF-C. RESULTS: The majority (70 of 84; 83.3% exhibited amplification of the c-myc gene. There were low-level amplifications in 63 (75.0% cases and high-level amplifications in 7 (8.3% cases. No amplification was found in 14 (16.7% cases. Tumors without c-myc amplification had lower VEGF-A, VEGF-C, and COX-2 expression levels than tumors with low-level and high-level c-myc amplification (statistically significant for VEGF-A; P = .0348. c-myc amplification was not correlated with clinicopathological parameters or survival. Only diffuse and mixed-type tumors, according to Lauren classification, exhibited c-myc amplifications more frequently (P = .0466. CONCLUSIONS: Amplifications of the c-myc gene are frequent in Barrett cancer. c-myc may be involved in the regulation of angiogenesis.

  1. Driving gradual endogenous c-myc overexpression by flow-sorting: intracellular signaling and tumor cell phenotype correlate with oncogene expression

    DEFF Research Database (Denmark)

    Knudsen, Kasper Jermiin; Holm, G.M.N.; Krabbe, J.S.

    2009-01-01

    Insulin-exposed rat mammary cancer cells were flow sorted based on a c-myc reporter plasmid encoding a destabilized green fluorescent protein. Sorted cells exhibited gradual increases in c-myc levels. Cells overexpressing c-myc by only 10% exhibited phenotypic changes attributable to c-myc overex...

  2. Nuclear localization of phosphorylated c-Myc protein in human tumor cells.

    Directory of Open Access Journals (Sweden)

    C. Soldani

    2010-05-01

    Full Text Available Using immunocytochemical techniques at light and electron microscopy, we analysed the distribution of phosphorylated c-Myc in actively proliferating human HeLa cells. The distribution pattern of c-Myc was also compared with those of other ribonucleoprotein (RNP-containing components (PANA, hnRNP-core proteins, fibrillarin or RNP-associated nuclear proteins (SC-35 splicing factor. Our results provide the first evidence that phosphorylated c-Myc accumulates in the nucleus of tumor cells, where it colocalizes with fibrillarin, both in the nucleolus and in extranucleolar structures.

  3. Cooverexpression of EpCAM and c-myc genes in malignant breast

    Indian Academy of Sciences (India)

    oncogene, affects progression, treatment, and diagnosis of many adenocarcinomas. C-myc has been shown to be a downstream target of EpCAM and is also one of the most important proto-oncogenes routinely overexpressed in breast cancer.

  4. Catching Comet's Particles in the Earth's Atmosphere by Using Balloons

    Science.gov (United States)

    Potashko, Oleksandr; Viso, Michel

    The project is intended to catch cometary particles in the atmosphere by using balloons. The investigation is based upon knowledge that the Earth crosses the comet’s tails during the year. One can catch these particles at different altitudes in the atmosphere. So, we will be able to gradually advance in the ability to launch balloons from low to high altitudes and try to catch particles from different comet tails. The maximum altitude that we have to reach is 40 km. Both methods - distance observation and cometary samples from mission Stardust testify to the presence of organic components in comet’s particles. It would be useful to know more details about this organic matter for astrobiology; besides, the factor poses danger to the Earth. Moreover, it is important to prove that it is possible to get fundamental scientific results at low cost. In the last 5 years launching balloons has become popular and this movement looks like hackers’ one - as most of them occur without launch permission to airspace. The popularity of ballooning is connected with low cost of balloon, GPS unit, video recording unit. If you use iPhone, you have a light solution with GPS, video, picture and control function in one unit. The price of balloon itself begins from $50; it depends on maximum altitude, payload weight and material. Many university teams realized balloon launching and reached even stratosphere at an altitude of 33 km. But most of them take only video and picture. Meanwhile, it is possible to carry out scientific experiments by ballooning, for example to collect comet particles. There is rich experience at the moment of the use of mineral, chemical and isotopic analysis techniques and data of the comet’s dust after successful landing of StarDust capsule with samples in 2006. Besides, we may use absolutely perfect material to catch particles in the atmosphere, which was used by cosmic missions such as Stardust and Japanese Hayabusa. As to balloon launches, we could use

  5. A model of gillnet catch in relation to the catchable biomass, saturation and soak time and sampling period

    Czech Academy of Sciences Publication Activity Database

    Prchalová, Marie; Mrkvička, T.; Peterka, Jiří; Čech, Martin; Berec, L.; Kubečka, Jan

    2011-01-01

    Roč. 107, 1-3 (2011), s. 201-209 ISSN 0165-7836 R&D Projects: GA AV ČR(CZ) KJB600960810 Institutional research plan: CEZ:AV0Z60170517 Keywords : catch interpretation * catch correction * video recording * reservoir * CPUE Subject RIV: DA - Hydrology ; Limnology Impact factor: 1.586, year: 2011

  6. Deepening Thermocline Displaces Salmon Catch On The Oregon Coast

    Science.gov (United States)

    Harrison, C. S.; Lawson, P.

    2015-12-01

    Establishing a linkage between fish stock distributions and physical oceanography at a fine scale provides insights into the dynamic nature of near-shore ocean habitats. Characterization of habitat preferences adds to our understanding of the ecosystem, and may improve forecasts of distribution for harvest management. The Project CROOS (Collaborative Research on Oregon Ocean Salmon) Chinook salmon catch data set represents an unprecedented high-resolution record of catch location and depth, with associated in-situ temperature measurements and stock identification derived from genetic data. Here we connect this data set with physical ocean observations to gain understanding of how circulation affects salmon catch distributions. The CROOS observations were combined with remote and in situ observations of temperature, as well as a data assimilative regional ocean model that incorporates satellite and HF radar data. Across the CROOS data set, catch is primarily located within the upwelling front over the seamounts and reef structures associated with Heceta and Stonewall Banks along the shelf break. In late September of 2014 the anomalously warm "blob" began to arrive on the Oregon coast coincident with a strong downwelling event. At this time the thermocline deepened from 20 to 40 m, associated with a deepening of salmon catch depth. A cold "bulb" of water over Heceta Bank may have provided a thermal refuge for salmon during the initial onshore movement of the anomalously warm water. These observations suggest that a warming ocean, and regional warming events in particular, will have large effects on fish distributions at local and regional scales, in turn impacting fisheries.

  7. Differential cellular responses by oncogenic levels of c-Myc expression in long-term confluent retinal pigment epithelial cells.

    Science.gov (United States)

    Wang, Yiping; Cheng, Xiangdong; Samma, Muhammad Kaleem; Kung, Sam K P; Lee, Clement M; Chiu, Sung Kay

    2018-06-01

    c-Myc is a highly pleiotropic transcription factor known to control cell cycle progression, apoptosis, and cellular transformation. Normally, ectopic expression of c-Myc is associated with promoting cell proliferation or triggering cell death via activating p53. However, it is not clear how the levels of c-Myc lead to different cellular responses. Here, we generated a series of stable RPE cell clones expressing c-Myc at different levels, and found that consistent low level of c-Myc induced cellular senescence by activating AP4 in post-confluent RPE cells, while the cells underwent cell death at high level of c-Myc. In addition, high level of c-Myc could override the effect of AP4 on cellular senescence. Further knockdown of AP4 abrogated senescence-like phenotype in cells expressing low level of c-Myc, and accelerated cell death in cells with medium level of c-Myc, indicating that AP4 was required for cellular senescence induced by low level of c-Myc.

  8. Protective and Catching Safety Systems In Construction

    Directory of Open Access Journals (Sweden)

    Kuzhin Marat

    2017-01-01

    Full Text Available In the article is described application of protective and catching systems in construction. Classification of similar systems, their types and purpose are listed. Dangerous zones on construction site and events to for limiting their influence or protection from the factors. Protective and catching systems is one of the most effective technical equipment, applied in recent time. Protective fences and catching systems are important part in the problem solution. Protective fences protect workers from falling from height. Protective and catching systems allows avoid injuries by workers, also catch debris, fallen from constructing buildings. In regard with continuing development in technical and technological solutions, protective and catching systems require adaptation to a new requirements of construction industry and requirements of normative documents. Technical regulations in the appliance sphere of protective and catching systems requires actualization and aligning with modern normatives. Important role should be given to developing organizational and technological documentation for application of the systems. Scientific studying of technical parameters of fences and protective catching nets also has great interest.

  9. Catching a Cold When It's Warm

    Science.gov (United States)

    ... Print this issue Catching a Cold When It’s Warm What’s the Deal with Summertime Sniffles? En español ... more unfair than catching a cold when it’s warm? How can cold symptoms arise when it’s not ...

  10. Tracing possible drivers of synchronously fluctuating species catches in individual logbook data

    Czech Academy of Sciences Publication Activity Database

    Jankovský, Martin; Boukal S., David; Pivnička, K.; Kubečka, Jan

    2011-01-01

    Roč. 18, č. 4 (2011), s. 297-306 ISSN 0969-997X R&D Projects: GA MŠk 7F10070 Grant - others:NFM(CZ) A/CZ0046/2/0029 Institutional research plan: CEZ:AV0Z60170517; CEZ:AV0Z50070508 Keywords : angler preferences * catch statistics * reservoirs * catch per unit effort * time series correlations Subject RIV: GL - Fishing Impact factor: 1.294, year: 2011

  11. Glutathione Depletion Induced by c-Myc Downregulation Triggers Apoptosis on Treatment with Alkylating Agents1

    Science.gov (United States)

    Biroccio, Annamaria; Benassi, Barbara; Fiorentino, Francesco; Zupi, Gabriella

    2004-01-01

    Abstract Here we investigate the mechanism(s) involved in the c-Myc-dependent drug response of melanoma cells. By using three M14-derived c-Myc low-expressing clones, we demonstrate that alkylating agents, cisplatin and melphalan, trigger apoptosis in the c-Myc antisense transfectants, but not in the parental line. On the contrary, topoisomerase inhibitors, adriamycin and camptothecin, induce apoptosis to the same extent regardless of c-Myc expression. Because we previously demonstrated that c-Myc downregulation decreases glutathione (GSH) content, we evaluated the role of GSH in the apoptosis induced by the different drugs. In control cells treated with one of the alkylating agents or the others, GSH depletion achieved by l-buthionine-sulfoximine preincubation opens the apoptotic pathway. The apoptosis proceeded through early Bax relocalization, cytochrome c release, and concomitant caspase-9 activation, whereas reactive oxygen species production and alteration of mitochondria membrane potential were late events. That GSH was determining in the c-Myc-dependent drug-induced apoptosis was demonstrated by altering the intracellular GSH content of the c-Myc low-expressing cells up to the level of controls. Indeed, GSH ethyl ester-mediated increase of GSH abrogated apoptosis induced by cisplatin and melphalan by inhibition of Bax/cytochrome c redistribution. The relationship among c-Myc, GSH content, and the response to alkylating agent has been also evaluated in the M14 Myc overexpressing clones as well as in the melanoma JR8 c-Myc antisense transfectants. All together, these results demonstrate that GSH plays a key role in governing c-Myc-dependent drug-induced apoptosis. PMID:15153331

  12. Interrelationship between chromosome 8 aneuploidy, C-MYC amplification and increased expression in individuals from northern Brazil with gastric adenocarcinoma

    Science.gov (United States)

    Calcagno, Danielle Queiroz; Leal, Mariana Ferreira; Seabra, Aline Damaceno; Khayat, André Salim; Chen, Elizabeth Suchi; Demachki, Samia; Assumpção, Paulo Pimentel; Faria, Mario Henrique Girão; Rabenhorst, Silvia Helena Barem; Ferreira, Márcia Valéria Pitombeira; Smith, Marília de Arruda Cardoso; Burbano, Rommel Rodríguez

    2006-01-01

    AIM: To investigate chromosome 8 numerical aberrations, C-MYC oncogene alterations and its expression in gastric cancer and to correlate these findings with histopathological characteristics of gastric tumors. METHODS: Specimens were collected surgically from seven patients with gastric adenocarcinomas. Immunostaining for C-MYC and dual-color fluorescence in situ hybridization (FISH) for C-MYC gene and chromosome 8 centromere were performed. RESULTS: All the cases showed chromosome 8 aneuploidy and C-MYC amplification, in both the diffuse and intestinal histopathological types of Lauren. No significant difference (P < 0.05) was observed between the level of chromosome 8 ploidy and the site, stage or histological type of the adenocarcinomas. C-MYC high amplification, like homogeneously stained regions (HSRs) and double minutes (DMs), was observed only in the intestinal-type. Structural rearrangement of C-MYC, like translocation, was observed only in the diffuse type. Regarding C-MYC gene, a significant difference (P < 0.05) was observed between the two histological types. The C-MYC protein was expressed in all the studied cases. In the intestinal-type the C-MYC immunoreactivity was localized only in the nucleus and in the diffuse type in the nucleus and cytoplasm. CONCLUSION: Distinct patterns of alterations between intestinal and diffuse types of gastric tumors support the hypothesis that these types follow different genetic pathways. PMID:17036397

  13. Reading for national development: Catching them young ...

    African Journals Online (AJOL)

    Reading for national development: Catching them young. ... It also discussed the impact of illiteracy and inefficient use of reading skills in an era of world's advanced technology. Some agencies that ... AJOL African Journals Online. HOW TO ...

  14. Timely Healthcare Checkup Catches Melanoma Early

    Science.gov (United States)

    ... please turn Javascript on. Feature: Skin Cancer Timely Healthcare Checkup Catches Melanoma Early Past Issues / Summer 2013 ... left the Congress and starting working as a healthcare consultant, when I finally decided to have a ...

  15. Evolutionarily conserved regions of the human c-myc protein can be uncoupled from transforming activity

    International Nuclear Information System (INIS)

    Sarid, J.; Halazonetis, T.D.; Murphy, W.; Leder, P.

    1987-01-01

    The myc family of oncogenes contains coding sequences that have been preserved in different species for over 400 million years. This conservation (which implies functional selection) is broadly represented throughout the C-terminal portion of the human c-myc protein but is largely restricted to three cluster of amino acid sequences in the N-terminal region. The authors have examined the role that the latter three regions of the c-myc protein might play in the transforming function of the c-myc gene. Several mutations, deletions and frameshifts, were introduced into the c-myc gene, and these mutant genes were tested for their ability to collaborate with the EJ-ras oncogene to transform rat embryo fibroblasts. Complete elimination of the first two N-terminal conserved segments abolished transforming activity. In contrast, genes altered in a portion of the second or the entire third conserved segment retained their transforming activity. Thus, the latter two segments are not required for the transformation process, suggesting that they serve another function related only to the normal expression of the c-myc gene

  16. Nutritional catch-up growth.

    Science.gov (United States)

    Gat-Yablonski, Galia; Pando, Rakefet; Phillip, Moshe

    2013-01-01

    Malnutrition, marked by variant nutrient deficiencies, is considered a leading cause of stunted growth worldwide. In developing countries, malnutrition is caused mainly by food shortage and infectious diseases. Malnutrition may also be found in the developed world, where it is due mostly to prematurity, chronic diseases, and anorexia nervosa. In most cases, when food consumption is corrected, spontaneous catch-up (CU) growth occurs. However, CU growth is not always complete, leading to growth deficits. Therefore, it is important to understand the mechanisms that govern this process. Using a rat model of food restriction followed by refeeding, we established a nutrition-induced CU growth model. Levels of leptin and insulin-like growth factor-1 were found to significantly decrease when food was restricted and to increase already 1 day after refeeding. Gene expression analysis of the growth plate revealed that food restriction specifically affects transcription factors such as the hypoxia inducible factor-1 and its downstream targets on the one hand, and global gene expression, indicating epigenetic regulation, on the other. Food restriction also reduced the level of several microRNAs, including the chondrocyte-specific miR-140, which led to an increase in its target, SIRT1, a class III histone deacetylase. These findings may explain the global changes in gene expression observed under nutritional manipulation. We suggest that multiple levels of regulation, including transcription factors, epigenetic mechanisms, and microRNAs respond to nutritional cues and offer a possible explanation for some of the effects of food restriction on epiphyseal growth plate growth. The means whereby these components sense changes in nutritional status are still unknown. Deciphering the role of epigenetic regulation in growth may pave the way for the development of new treatments for children with growth disorders. Copyright © 2013 S. Karger AG, Basel.

  17. Effect of survey design and catch rate estimation on total catch estimates in Chinook salmon fisheries

    Science.gov (United States)

    McCormick, Joshua L.; Quist, Michael C.; Schill, Daniel J.

    2012-01-01

    Roving–roving and roving–access creel surveys are the primary techniques used to obtain information on harvest of Chinook salmon Oncorhynchus tshawytscha in Idaho sport fisheries. Once interviews are conducted using roving–roving or roving–access survey designs, mean catch rate can be estimated with the ratio-of-means (ROM) estimator, the mean-of-ratios (MOR) estimator, or the MOR estimator with exclusion of short-duration (≤0.5 h) trips. Our objective was to examine the relative bias and precision of total catch estimates obtained from use of the two survey designs and three catch rate estimators for Idaho Chinook salmon fisheries. Information on angling populations was obtained by direct visual observation of portions of Chinook salmon fisheries in three Idaho river systems over an 18-d period. Based on data from the angling populations, Monte Carlo simulations were performed to evaluate the properties of the catch rate estimators and survey designs. Among the three estimators, the ROM estimator provided the most accurate and precise estimates of mean catch rate and total catch for both roving–roving and roving–access surveys. On average, the root mean square error of simulated total catch estimates was 1.42 times greater and relative bias was 160.13 times greater for roving–roving surveys than for roving–access surveys. Length-of-stay bias and nonstationary catch rates in roving–roving surveys both appeared to affect catch rate and total catch estimates. Our results suggest that use of the ROM estimator in combination with an estimate of angler effort provided the least biased and most precise estimates of total catch for both survey designs. However, roving–access surveys were more accurate than roving–roving surveys for Chinook salmon fisheries in Idaho.

  18. Genetic and genomic analysis modeling of germline c-MYC overexpression and cancer susceptibility

    Directory of Open Access Journals (Sweden)

    Nunes Virginia

    2008-01-01

    Full Text Available Abstract Background Germline genetic variation is associated with the differential expression of many human genes. The phenotypic effects of this type of variation may be important when considering susceptibility to common genetic diseases. Three regions at 8q24 have recently been identified to independently confer risk of prostate cancer. Variation at 8q24 has also recently been associated with risk of breast and colorectal cancer. However, none of the risk variants map at or relatively close to known genes, with c-MYC mapping a few hundred kilobases distally. Results This study identifies cis-regulators of germline c-MYC expression in immortalized lymphocytes of HapMap individuals. Quantitative analysis of c-MYC expression in normal prostate tissues suggests an association between overexpression and variants in Region 1 of prostate cancer risk. Somatic c-MYC overexpression correlates with prostate cancer progression and more aggressive tumor forms, which was also a pathological variable associated with Region 1. Expression profiling analysis and modeling of transcriptional regulatory networks predicts a functional association between MYC and the prostate tumor suppressor KLF6. Analysis of MYC/Myc-driven cell transformation and tumorigenesis substantiates a model in which MYC overexpression promotes transformation by down-regulating KLF6. In this model, a feedback loop through E-cadherin down-regulation causes further transactivation of c-MYC. Conclusion This study proposes that variation at putative 8q24 cis-regulator(s of transcription can significantly alter germline c-MYC expression levels and, thus, contribute to prostate cancer susceptibility by down-regulating the prostate tumor suppressor KLF6 gene.

  19. c-Myc is essential to prevent endothelial pro-inflammatory senescent phenotype.

    Directory of Open Access Journals (Sweden)

    Victoria Florea

    Full Text Available The proto-oncogene c-Myc is vital for vascular development and promotes tumor angiogenesis, but the mechanisms by which it controls blood vessel growth remain unclear. In the present work we investigated the effects of c-Myc knockdown in endothelial cell functions essential for angiogenesis to define its role in the vasculature. We provide the first evidence that reduction in c-Myc expression in endothelial cells leads to a pro-inflammatory senescent phenotype, features typically observed during vascular aging and pathologies associated with endothelial dysfunction. c-Myc knockdown in human umbilical vein endothelial cells using lentivirus expressing specific anti-c-Myc shRNA reduced proliferation and tube formation. These functional defects were associated with morphological changes, increase in senescence-associated-β-galactosidase activity, upregulation of cell cycle inhibitors and accumulation of c-Myc-deficient cells in G1-phase, indicating that c-Myc knockdown in endothelial cells induces senescence. Gene expression analysis of c-Myc-deficient endothelial cells showed that senescent phenotype was accompanied by significant upregulation of growth factors, adhesion molecules, extracellular-matrix components and remodeling proteins, and a cluster of pro-inflammatory mediators, which include Angptl4, Cxcl12, Mdk, Tgfb2 and Tnfsf15. At the peak of expression of these cytokines, transcription factors known to be involved in growth control (E2f1, Id1 and Myb were downregulated, while those involved in inflammatory responses (RelB, Stat1, Stat2 and Stat4 were upregulated. Our results demonstrate a novel role for c-Myc in the prevention of vascular pro-inflammatory phenotype, supporting an important physiological function as a central regulator of inflammation and endothelial dysfunction.

  20. Macrocyclic peptides decrease c-Myc protein levels and reduce prostate cancer cell growth.

    Science.gov (United States)

    Mukhopadhyay, Archana; Hanold, Laura E; Thayele Purayil, Hamsa; Gisemba, Solomon A; Senadheera, Sanjeewa N; Aldrich, Jane V

    2017-08-03

    The oncoprotein c-Myc is often overexpressed in cancer cells, and the stability of this protein has major significance in deciding the fate of a cell. Thus, targeting c-Myc levels is an attractive approach for developing therapeutic agents for cancer treatment. In this study, we report the anti-cancer activity of the macrocyclic peptides [D-Trp]CJ-15,208 (cyclo[Phe-D-Pro-Phe-D-Trp]) and the natural product CJ-15,208 (cyclo[Phe-D-Pro-Phe-Trp]). [D-Trp]CJ-15,208 reduced c-Myc protein levels in prostate cancer cells and decreased cell proliferation with IC 50 values ranging from 2.0 to 16 µM in multiple PC cell lines. [D-Trp]CJ-15,208 induced early and late apoptosis in PC-3 cells following 48 hours treatment, and growth arrest in the G2 cell cycle phase following both 24 and 48 hours treatment. Down regulation of c-Myc in PC-3 cells resulted in loss of sensitivity to [D-Trp]CJ-15,208 treatment, while overexpression of c-Myc in HEK-293 cells imparted sensitivity of these cells to [D-Trp]CJ-15,208 treatment. This macrocyclic tetrapeptide also regulated PP2A by reducing the levels of its phosphorylated form which regulates the stability of cellular c-Myc protein. Thus [D-Trp]CJ-15,208 represents a new lead compound for the potential development of an effective treatment of prostate cancer.

  1. Inhibition of c-Myc overcomes cytotoxic drug resistance in acute myeloid leukemia cells by promoting differentiation.

    Directory of Open Access Journals (Sweden)

    Xiao-Na Pan

    Full Text Available Nowadays, drug resistance still represents a major obstacle to successful acute myeloid leukemia (AML treatment and the underlying mechanism is not fully elucidated. Here, we found that high expression of c-Myc was one of the cytogenetic characteristics in the drug-resistant leukemic cells. c-Myc over-expression in leukemic cells induced resistance to chemotherapeutic drugs, enhanced colony formation capacity and inhibited cell differentiation induced by all-trans retinoic acid (ATRA. Meanwhile, inhibition of c-Myc by shRNA or specific c-Myc inhibitor 10058-F4 rescued the sensitivity to cytotoxic drugs, restrained the colony formation ability and promoted differentiation. RT-PCR and western blotting analysis showed that down-regulation of C/EBPβ contributed to the poor differentiation state of leukemic cells induced by c-Myc over-expression. Importantly, over-expression of C/EBPβ could reverse c-Myc induced drug resistance. In primary AML cells, the c-Myc expression was negatively correlated with C/EBPβ. 10058-F4, displayed anti-proliferative activity and increased cellular differentiation with up-regulation of C/EBPβ in primary AML cells. Thus, our study indicated that c-Myc could be a novel target to overcome drug resistance, providing a new approach in AML therapy.

  2. Overexpression and amplification of the c-myc gene in mouse tumors induced by chemical and radiations

    Energy Technology Data Exchange (ETDEWEB)

    Niwa, Ohtsura; Enoki, Yoshitaka; Yokoro, Kenjiro

    1989-03-01

    We examined expression of the c-myc gene by the dot blot hybridization of total cellular RNA from mouse primary tumors induced by chemicals and radiations. Expression of the c-myc gene was found to be elevated in 69 cases among 177 independently induced tumors of 12 different types. DNA from tumors overexpressing the myc gene was analyzed by Southern blotting. No case of rearrangement was detected. However, amplification of the c-myc gene was found in 7 cases of primary sarcomas. These included 4 cases out of 24 methylcholanthrene-induced sarcomas and 3 cases out of 7 /alpha/-tocopherol-induced sacromas. We also analyzed 8 cases of sarcomas induced by radiations, but could not find changes in the gene structure of the c-myc gene. Thus, our data indicate tumor type specificity and agent specificity of c-myc gene amplification. (author).

  3. Hydrology and angler’s catches in the Czech reservoirs

    Czech Academy of Sciences Publication Activity Database

    Draštík, Vladislav; Kubečka, Jan; Šovčík, P.

    2004-01-01

    Roč. 4, č. 4 (2004), s. 429-439 ISSN 1642-3593. [Ecohydrology and physical fish habitat modifikation in lakes. Mondsee, 26.11.2003-28.11.2003] R&D Projects: GA ČR(CZ) GA206/02/0520; GA AV ČR(CZ) IBS6017004; GA AV ČR(CZ) KSK6005114 Institutional research plan: CEZ:AV0Z6017912 Keywords : angler’s catch * fish species composition * bream/perch ratio Subject RIV: DA - Hydrology ; Limnology

  4. Shikonin regulates C-MYC and GLUT1 expression through the MST1-YAP1-TEAD1 axis

    Energy Technology Data Exchange (ETDEWEB)

    Vališ, Karel, E-mail: karel.valis@biomed.cas.cz [Laboratory of Structural Biology and Cell Signaling, Institute of Microbiology, v.v.i., The Czech Academy of Sciences, Prague (Czech Republic); Faculty of Science, Charles University, Prague (Czech Republic); Talacko, Pavel; Grobárová, Valéria [Laboratory of Structural Biology and Cell Signaling, Institute of Microbiology, v.v.i., The Czech Academy of Sciences, Prague (Czech Republic); Faculty of Science, Charles University, Prague (Czech Republic); Černý, Jan [Faculty of Science, Charles University, Prague (Czech Republic); Novák, Petr, E-mail: pnovak@biomed.cas.cz [Laboratory of Structural Biology and Cell Signaling, Institute of Microbiology, v.v.i., The Czech Academy of Sciences, Prague (Czech Republic); Faculty of Science, Charles University, Prague (Czech Republic)

    2016-12-10

    The general mechanism underlying the tumor suppressor activity of the Hippo signaling pathway remains unclear. In this study, we explore the molecular mechanisms connecting the Hippo signaling pathway with glucose metabolism. We have found that two key regulators of glycolysis, C-MYC and GLUT1, are targets of the Hippo signaling pathway in human leukemia cells. Our results revealed that activation of MST1 by the natural compound shikonin inhibited the expression of GLUT1 and C-MYC. Furthermore, RNAi experiments confirmed the regulation of GLUT1 and C-MYC expression via the MST1-YAP1-TEAD1 axis. Surprisingly, YAP1 was found to positively regulate C-MYC mRNA levels in complex with TEAD1, while it negatively regulates C-MYC levels in cooperation with MST1. Hence, YAP1 serves as a rheostat for C-MYC, which is regulated by MST1. In addition, depletion of MST1 stimulates lactate production, whereas the specific depletion of TEAD1 has an opposite effect. The inhibition of lactate production and cellular proliferation induced by shikonin also depends on the Hippo pathway activity. Finally, a bioinformatic analysis revealed conserved TEAD-binding motifs in the C-MYC and GLUT1 promoters providing another molecular data supporting our observations. In summary, regulation of glucose metabolism could serve as a new tumor suppressor mechanism orchestrated by the Hippo signaling pathway. - Highlights: • Shikonin inhibits C-MYC and GLUT1 expression in MST1 and YAP1 dependent manner. • YAP1-TEAD1 interaction activates C-MYC and GLUT1 expression. • MST1 in cooperation with YAP1 inhibits C-MYC and GLUT1 expression. • MST1-YAP1-TEAD1 axis regulates lactate production by leukemic cells. • MST1 and YAP1 proteins block proliferation of leukemic cells.

  5. Low expression of c-Myc protein predicts poor outcomes in patients with hepatocellular carcinoma after resection.

    Science.gov (United States)

    Ji, Fei; Zhang, Zhi-Heng; Zhang, Yi; Shen, Shun-Li; Cao, Qing-Hua; Zhang, Long-Juan; Li, Shao-Qiang; Peng, Bao-Gang; Liang, Li-Jian; Hua, Yun-Peng

    2018-04-24

    Embryonic Liver Fodrin (ELF) is an adaptor protein of transforming growth factor (TGF-β) signaling cascade. Disruption of ELF results in mislocalization of Smad3 and Smad4, leading to compromised TGF-β signaling. c-Myc is an important oncogenic transcription factor, and the disruption of TGF-β signaling promotes c-Myc-induced hepatocellular carcinoma (HCC) carcinogenesis. However, the prognostic significance of c-Myc in HCC is less understood METHODS: The expression of c-Myc protein and mRNA were measured by immunohistochemistry (IHC) and qRT- PCR, respectively. IHC was performed to detect TGF-β1 and ELF expression in HCC tissues. Their relationship with clinicopathological factors and overall survival (OS) and disease free survival (DFS) were examined. The expression of c-Myc protein and mRNA in HCC tissues were significantly higher in HCC area than those in normal liver tissues. However, the expression were low compared with those adjacent to HCC area. c-Myc protein was independently predictive of DFS and OS, and it was negatively correlated with tumor size (P = 0.031), tumor number (P = 0.038), and recurrence (P = 0.001). Low c-Myc expression was associated with short-term recurrence and poor prognosis. The predictive value of c-Myc combined with TGF-β1 or/and ELF was higher than that of any other single marker. Low c-Myc, high TGF-β1 or/and low ELF expression was associated with the worst DFS and OS. Low expression of c-Myc protein predicts poor outcomes in patients with HCC with hepatectomy. The combination of the expression of c-Myc, TGF-β1, and ELF can be used to accurately predict outcomes of patients with HCC.

  6. Endogenous c-Myc is essential for p53-induced apoptosis in response to DNA damage in vivo.

    Science.gov (United States)

    Phesse, T J; Myant, K B; Cole, A M; Ridgway, R A; Pearson, H; Muncan, V; van den Brink, G R; Vousden, K H; Sears, R; Vassilev, L T; Clarke, A R; Sansom, O J

    2014-06-01

    Recent studies have suggested that C-MYC may be an excellent therapeutic cancer target and a number of new agents targeting C-MYC are in preclinical development. Given most therapeutic regimes would combine C-MYC inhibition with genotoxic damage, it is important to assess the importance of C-MYC function for DNA damage signalling in vivo. In this study, we have conditionally deleted the c-Myc gene in the adult murine intestine and investigated the apoptotic response of intestinal enterocytes to DNA damage. Remarkably, c-Myc deletion completely abrogated the immediate wave of apoptosis following both ionizing irradiation and cisplatin treatment, recapitulating the phenotype of p53 deficiency in the intestine. Consistent with this, c-Myc-deficient intestinal enterocytes did not upregulate p53. Mechanistically, this was linked to an upregulation of the E3 Ubiquitin ligase Mdm2, which targets p53 for degradation in c-Myc-deficient intestinal enterocytes. Further, low level overexpression of c-Myc, which does not impact on basal levels of apoptosis, elicited sustained apoptosis in response to DNA damage, suggesting c-Myc activity acts as a crucial cell survival rheostat following DNA damage. We also identify the importance of MYC during DNA damage-induced apoptosis in several other tissues, including the thymus and spleen, using systemic deletion of c-Myc throughout the adult mouse. Together, we have elucidated for the first time in vivo an essential role for endogenous c-Myc in signalling DNA damage-induced apoptosis through the control of the p53 tumour suppressor protein.

  7. Catch shares slow the race to fish

    Science.gov (United States)

    Birkenbach, Anna M.; Kaczan, David J.; Smith, Martin D.

    2017-04-01

    In fisheries, the tragedy of the commons manifests as a competitive race to fish that compresses fishing seasons, resulting in ecological damage, economic waste, and occupational hazards. Catch shares are hypothesized to halt the race by securing each individual’s right to a portion of the total catch, but there is evidence for this from selected examples only. Here we systematically analyse natural experiments to test whether catch shares reduce racing in 39 US fisheries. We compare each fishery treated with catch shares to an individually matched control before and after the policy change. We estimate an average policy treatment effect in a pooled model and in a meta-analysis that combines separate estimates for each treatment-control pair. Consistent with the theory that market-based management ends the race to fish, we find strong evidence that catch shares extend fishing seasons. This evidence informs the current debate over expanding the use of market-based regulation to other fisheries.

  8. Storage of catch crops to produce biogas

    DEFF Research Database (Denmark)

    Molinuevo-Salces, Beatriz; Ahring, Birgitte Kiær; Uellendahl, Hinrich

    2014-01-01

    . On the contrary, the poor quality of IR silage, due to its high TS content, made it inappropriate as feedstock for biogas production. A TS content of 25-35% is preferable, to obtain a proper fermentation avoid leachate run-off and growth of Clostridium sp. or mold formation. Avoiding soil particles in the bales......Catch crop biomass is a promising co-substrate for manure-based biogas plants in Denmark since the cultivation of catch crops is mandatory to retain nutrients in the soil, contributing to protect the aquatic environment. In general, the growth period for catch crops is from harvest of the previous...... crop in July-August to the end of the growing season and harvest in late October. Hence, for use of the biomass in biogas production there is a need for storage of the biomass. Storage as silage would guarantee the availability of the feedstock for biogas production during the whole year. A proper...

  9. Targeting C-Myc Promoter: Helquats As Novel G-Quadruplex Stabilizing Ligands

    Czech Academy of Sciences Publication Activity Database

    Kužmová, Erika; Kozák, Jaroslav; Komárková, Veronika; Pytlík, R.; Teplý, Filip; Hájek, Miroslav

    2014-01-01

    Roč. 124, č. 21 (2014) ISSN 0006-4971. [Annual Meeting of the American Society of Hematology /56./. 06.12.2014-09.12.2014, San Francisco] Institutional support: RVO:61388963 Keywords : helquats * C-Myc * leukemia Subject RIV: CE - Biochemistry

  10. Gamabufotalin triggers c-Myc degradation via induction of WWP2 in multiple myeloma cells.

    Science.gov (United States)

    Yu, Zhenlong; Li, Tao; Wang, Chao; Deng, Sa; Zhang, Baojing; Huo, Xiaokui; Zhang, Bo; Wang, Xiaobo; Zhong, Yuping; Ma, Xiaochi

    2016-03-29

    Deciding appropriate therapy for multiple myeloma (MM) is challenging because of the occurrence of multiple chromosomal changes and the fatal nature of the disease. In the current study, gamabufotalin (GBT) was isolated from toad venom, and its tumor-specific cytotoxicity was investigated in human MM cells. We found GBT inhibited cell growth and induced apoptosis with the IC50 values <50 nM. Mechanistic studies using functional approaches identified GBT as an inhibitor of c-Myc. Further analysis showed that GBT especially evoked the ubiquitination and degradation of c-Myc protein, thereby globally repressing the expression of c-Myc target genes. GBT treatment inhibited ERK and AKT signals, while stimulating the activation of JNK cascade. An E3 ubiquitin-protein ligase, WWP2, was upregulated following JNK activation and played an important role in c-Myc ubiquitination and degradation through direct protein-protein interaction. The antitumor effect of GBT was validated in a xenograft mouse model and the suppression of MM-induced osteolysis was verified in a SCID-hu model in vivo. Taken together, our study identified the potential of GBT as a promising therapeutic agent in the treatment of MM.

  11. Aberrant immunoglobulin and c-myc gene rearrangements in patients with nonmalignant monoclonal cryoglobulinemia

    International Nuclear Information System (INIS)

    Perl, A.; Wang, N.; Williams, J.M.; Hunt, M.J.; Rosenfeld, S.I.; Condemi, J.J.; Packman, C.H.; Abraham, G.N.

    1987-01-01

    The status of the immunoglobulin (Ig) genes was investigated in patients with idiopathic nonmalignant monoclonal IgG cryoglobulinemia (NCG). In NCG, monoclonal antibodies are synthesized at an accelerated rate by nonmalignant B lymphocytes. In order to determine whether this high production rate is related to a clonal B cell expansion, the rearrangement of the Ig genes was investigated by Southern blot analysis of genomic, 32 P-labelled, DNA extracted from the peripheral blood lymphocytes of four NCG patients. In three of four (VI, BR, and CH) clonal expansion of B cells was detected using probes specific for the genes. BamHI digestion of DNA from VI and BR produced three rearranged fragments which cohybridized with two of the probes. This finding suggested the presence of additional nonsecretory B cell clones and/or disruption of the gene segments spanned by and detected with the probes. In addition, the possibility of aberrant gene rearrangements was supported by noting the alteration of the c-myc gene locus in genomic DNA from peripheral blood leukocytes of VI and CH. Northern blot analysis of RNA isolated from peripheral blood B cells of VI and CH demonstrated aberrant transcripts of the c-myc gene, showing an active role of the altered c-myc locus. Detection of c-myc rearrangement in NCG patients clearly shows that this event may not be a final step in malignant B cell transformation

  12. c-Myc over-expression in Ramos Burkitt's lymphoma cell line predisposes to iron homeostasis disruption in vitro

    International Nuclear Information System (INIS)

    Habel, Marie-Eve; Jung, Daniel

    2006-01-01

    Burkitt's lymphoma is an aggressive B-cell neoplasm resulting from deregulated c-myc expression. We have previously shown that proliferation of Burkitt's lymphoma cell lines such as Ramos is markedly reduced by iron treatment. It has been shown that iron induces expression of c-myc which, owing to its transcriptional regulatory functions, regulates genes involved in iron metabolism. Transient enhancement of c-myc expression by iron could increase the expression of genes involved in iron incorporation, which could lead to an accumulation of intracellular free iron. Here, we have investigated whether cells with a high basal level of c-Myc were more likely to accumulate free iron. Our results suggest that the basal level of c-Myc in Ramos cells is twofold higher than what is seen in HL-60 cells. Moreover, in Ramos cells, where c-Myc is expressed at a high level, H-ferritin expression is down-regulated, transferrin receptor (CD71) expression is increased, and ferritin translation is inhibited. These modifications in iron metabolism, resulting from the strong basal expression of c-Myc, and amplified by iron addition, could lead to a disruption in homeostasis and consequently to growth arrest

  13. Inversed relationship between CD44 variant and c-Myc due to oxidative stress-induced canonical Wnt activation

    International Nuclear Information System (INIS)

    Yoshida, Go J.; Saya, Hideyuki

    2014-01-01

    Highlights: •CD44 variant8–10 and c-Myc are inversely expressed in gastric cancer cells. •Redox-stress enhances c-Myc expression via canonical Wnt signal. •CD44v, but not CD44 standard, suppresses redox stress-induced Wnt activation. •CD44v expression promotes both transcription and proteasome degradation of c-Myc. •Inversed expression pattern between CD44v and c-Myc is often recognized in vivo. -- Abstract: Cancer stem-like cells express high amount of CD44 variant8-10 which protects cancer cells from redox stress. We have demonstrated by immunohistochemical analysis and Western blotting, and reverse-transcription polymerase chain reaction, that CD44 variant8-10 and c-Myc tend to show the inversed expression manner in gastric cancer cells. That is attributable to the oxidative stress-induced canonical Wnt activation, and furthermore, the up-regulation of the downstream molecules, one of which is oncogenic c-Myc, is not easily to occur in CD44 variant-positive cancer cells. We have also found out that CD44v8-10 expression is associated with the turn-over of the c-Myc with the experiments using gastric cancer cell lines. This cannot be simply explained by the model of oxidative stress-induced Wnt activation. CD44v8-10-positive cancer cells are enriched at the invasive front. Tumor tissue at the invasive area is considered to be composed of heterogeneous cellular population; dormant cancer stem-like cells with CD44v8-10 high / Fbw7 high / c-Myc low and proliferative cancer stem-like cells with CD44v8-10 high / Fbw7 low / c-Myc high

  14. Inversed relationship between CD44 variant and c-Myc due to oxidative stress-induced canonical Wnt activation

    Energy Technology Data Exchange (ETDEWEB)

    Yoshida, Go J., E-mail: medical21go@yahoo.co.jp; Saya, Hideyuki

    2014-01-10

    Highlights: •CD44 variant8–10 and c-Myc are inversely expressed in gastric cancer cells. •Redox-stress enhances c-Myc expression via canonical Wnt signal. •CD44v, but not CD44 standard, suppresses redox stress-induced Wnt activation. •CD44v expression promotes both transcription and proteasome degradation of c-Myc. •Inversed expression pattern between CD44v and c-Myc is often recognized in vivo. -- Abstract: Cancer stem-like cells express high amount of CD44 variant8-10 which protects cancer cells from redox stress. We have demonstrated by immunohistochemical analysis and Western blotting, and reverse-transcription polymerase chain reaction, that CD44 variant8-10 and c-Myc tend to show the inversed expression manner in gastric cancer cells. That is attributable to the oxidative stress-induced canonical Wnt activation, and furthermore, the up-regulation of the downstream molecules, one of which is oncogenic c-Myc, is not easily to occur in CD44 variant-positive cancer cells. We have also found out that CD44v8-10 expression is associated with the turn-over of the c-Myc with the experiments using gastric cancer cell lines. This cannot be simply explained by the model of oxidative stress-induced Wnt activation. CD44v8-10-positive cancer cells are enriched at the invasive front. Tumor tissue at the invasive area is considered to be composed of heterogeneous cellular population; dormant cancer stem-like cells with CD44v8-10 {sup high}/ Fbw7 {sup high}/ c-Myc {sup low} and proliferative cancer stem-like cells with CD44v8-10 {sup high}/ Fbw7 {sup low}/ c-Myc {sup high}.

  15. Hunting the Shadow, Catching the Light

    DEFF Research Database (Denmark)

    Toft, Anne Elisabeth; Nielsen, Torben

    2009-01-01

    From 28 October to 6 November 2009 twenty-one 3rd year students in interior design from Central Academy of Fine Arts (CAFA), School of Architecture, Beijing participated in the workshop Hunting the Shadow - Catching the Light. The workshop was conceived and led by the Danish architects Torben Nie...

  16. Catching up with the Future of Physics

    CERN Multimedia

    2008-01-01

    With most of the high-energy physics world on tenterhooks waiting for the startup of the Large Hadron Collider in Switzerland later this year, it's a good time to catch up (if you haven't already) on what all the excitement is about.

  17. Pitch-catch only ultrasonic fluid densitometer

    Science.gov (United States)

    Greenwood, M.S.; Harris, R.V.

    1999-03-23

    The present invention is an ultrasonic fluid densitometer that uses a material wedge and pitch-catch only ultrasonic transducers for transmitting and receiving ultrasonic signals internally reflected within the material wedge. Density of a fluid is determined by immersing the wedge into the fluid and measuring reflection of ultrasound at the wedge-fluid interface. 6 figs.

  18. 75 FR 41996 - Fisheries of the Northeastern United States; Northeast Multispecies Fishery; Pollock Catch Limit...

    Science.gov (United States)

    2010-07-20

    ... projections and catch advice. The 2010 pollock benchmark stock assessment was scheduled as soon as practicable... reviewed benchmark stock assessment review (SAW 50) was completed during the first week of June 2010, and... economic opportunity that otherwise might be foregone. The new information from the pollock benchmark stock...

  19. Contractile fibers and catch bond clusters : a biological force sensor?

    NARCIS (Netherlands)

    Novikova, E.A.; Storm, C.

    2013-01-01

    Catch bonds are cellular receptor-ligand pairs whose lifetime, counterintuitively, increases with increasing load. Although their existence was initially pure theoretical speculation, recent years have seen several experimental demonstrations of catch-bond behavior in biologically relevant and

  20. Test plan for measuring ventilation rates and combustible gas levels in RPP active catch tanks

    Energy Technology Data Exchange (ETDEWEB)

    NGUYEN, D.M.

    1999-06-03

    The purpose of this test is to provide an initial screening of combustible gas concentrations in catch tanks that currently are operated by River Protection Project (RPP). The data will be used to determine whether or not additional data will be needed for closure of the flammable gas unreviewed safety question for these facilities. This test will involve field measurements of ammonia, organic vapor, and total combustible gas levels in the headspace of the catch tanks. If combustible gas level in a tank exceeds an established threshold, gas samples will be collected in SUMMA canisters for more extensive laboratory analysis. In addition, ventilation rates of some catch tanks will be measured to evaluate removal of flammable gas by air flow through the tanks.

  1. Synthesis of Seafood Catch, Distribution, and Consumption Patterns in the Gulf of Mexico Region

    International Nuclear Information System (INIS)

    Steimle and Associates, Inc.

    1999-01-01

    The purpose of this task was to gather and assemble information that will provide a synthesis of seafood catch, distribution and consumption patterns for the Gulf of Mexico (GOM) region. This task was part of a U.S. Department of Energy (DOE)-sponsored project entitled ''Environmental and Economic Assessment of Discharges from Gulf of Mexico Region Oil and Gas Operations.'' Personal interviews were conducted with a total of 905 recreational fishermen and 218 commercial fishermen (inclusive of shrimpers, crabbers, oystermen and finfishermen) in Louisiana and Texas using survey questionnaires developed for the study. Results of these interviews detail the species and quantities caught, location of catch, mode of fishing, distribution of catch, family consumption patterns and demographics of the fishermen

  2. Synthesis of Seafood Catch, Distribution, and Consumption Patterns in the Gulf of Mexico Region

    Energy Technology Data Exchange (ETDEWEB)

    Steimle and Associates, Inc.

    1999-08-16

    The purpose of this task was to gather and assemble information that will provide a synthesis of seafood catch, distribution and consumption patterns for the Gulf of Mexico (GOM) region. This task was part of a U.S. Department of Energy (DOE)-sponsored project entitled ''Environmental and Economic Assessment of Discharges from Gulf of Mexico Region Oil and Gas Operations.'' Personal interviews were conducted with a total of 905 recreational fishermen and 218 commercial fishermen (inclusive of shrimpers, crabbers, oystermen and finfishermen) in Louisiana and Texas using survey questionnaires developed for the study. Results of these interviews detail the species and quantities caught, location of catch, mode of fishing, distribution of catch, family consumption patterns and demographics of the fishermen.

  3. The 5T mouse multiple myeloma model: Absence of c-myc oncogene rearrangement in early transplant generations

    NARCIS (Netherlands)

    Radl, J.; Punt, Y.A.; Enden-Vieveen, M.H.M. van den; Bentvelzen, P.A.J.; Bakkus, M.H.C.; Akker T., W. van den; Benner, R.

    1990-01-01

    Consistent chromosomal translocations involving the c-myc cellular oncogene and one of the three immunoglobulin loci are typical for human Burkitt's lymphoma, induced mouse plasmacytoma (MPC) and spontaneously arising rat immunocytoma (RIC). Another plasma cell malignancy, multiple myeloma (MM),

  4. Spatiotemporal characteristics of muscle patterns for ball catching

    Directory of Open Access Journals (Sweden)

    Mattia eD'Andola

    2013-08-01

    Full Text Available What sources of information and what control strategies the CNS uses to perform movements that require accurate sensorimotor coordination, such as catching a flying ball, is still debated. Here we analyzed the EMG waveforms recorded from 16 shoulder and elbow muscles in six subjects during catching of balls projected frontally from a distance of 6 m and arriving at two different heights and with three different flight times (550, 650, 750 ms. We found that a large fraction of the variation in the muscle patterns was captured by two time-varying muscle synergies, coordinated recruitment of groups of muscles with specific activation waveforms, modulated in amplitude and shifted in time according to the ball’s arrival height and flight duration. One synergy was recruited with a short and fixed delay from launch time. Remarkably, a second synergy was recruited at a fixed time before impact, suggesting that it is timed according to an accurate time-to-contact estimation. These results suggest that the control of interceptive movements relies on a combination of reactive and predictive processes through the intermittent recruitment of time-varying muscle synergies. Knowledge of the dynamic effect of gravity and drag on the ball may be then implicitly incorporated in a direct mapping of visual information into a small number of synergy recruitment parameters.

  5. c-MYC expression sensitizes medulloblastoma cells to radio- and chemotherapy and has no impact on response in medulloblastoma patients

    Directory of Open Access Journals (Sweden)

    Stearns Duncan

    2011-02-01

    Full Text Available Abstract Background To study whether and how c-MYC expression determines response to radio- and chemotherapy in childhood medulloblastoma (MB. Methods We used DAOY and UW228 human MB cells engineered to stably express different levels of c-MYC, and tested whether c-MYC expression has an effect on radio- and chemosensitivity using the colorimetric 3-(4,5-dimethylthiazol-2-yl-5-(3-carboxymethoxyphenyl-2-(4-sulfophenyl-2H-tetrazolium inner salt (MTS assay, clonogenic survival, apoptosis assays, cell cycle analysis, and western blot assessment. In an effort to validate our results, we analyzed c-MYC mRNA expression in formalin-fixed paraffin-embedded tumor samples from well-documented patients with postoperative residual tumor and compared c-MYC mRNA expression with response to radio- and chemotherapy as examined by neuroradiological imaging. Results In DAOY - and to a lesser extent in UW228 - cells expressing high levels of c-MYC, the cytotoxicity of cisplatin, and etoposide was significantly higher when compared with DAOY/UW228 cells expressing low levels of c-MYC. Irradiation- and chemotherapy-induced apoptotic cell death was enhanced in DAOY cells expressing high levels of c-MYC. The response of 62 of 66 residual tumors was evaluable and response to postoperative radio- (14 responders (CR, PR vs. 5 non-responders (SD, PD or chemotherapy (23 CR/PR vs. 20 SD/PD was assessed. c-MYC mRNA expression was similar in primary MB samples of responders and non-responders (Mann-Whitney U test, p = 0.50, ratio 0.49, 95% CI 0.008-30.0 and p = 0.67, ratio 1.8, 95% CI 0.14-23.5, respectively. Conclusions c-MYC sensitizes MB cells to some anti-cancer treatments in vitro. As we failed to show evidence for such an effect on postoperative residual tumors when analyzed by imaging, additional investigations in xenografts and larger MB cohorts may help to define the exact function of c-MYC in modulating response to treatment.

  6. Histopathological and molecular prognostic markers in medulloblastoma: c-myc, N-myc, TrkC, and anaplasia.

    Science.gov (United States)

    Eberhart, Charles G; Kratz, John; Wang, Yunyue; Summers, Krista; Stearns, Duncan; Cohen, Kenneth; Dang, Chi V; Burger, Peter C

    2004-05-01

    Several molecular and histopathological prognostic markers have been proposed for the therapeutic stratification of medulloblastoma patients. Amplification of the c-myc oncogene, elevated levels of c-myc mRNA, or tumor anaplasia have been associated with worse clinical outcomes. In contrast, high TrkC mRNA expression generally presages longer survival. The goal of this study was to evaluate the prognostic value of c-myc, N-myc and TrkC expression in medulloblastomas and compare them to histopathological classification. We used in situ hybridization to measure expression of these molecular markers. c-myc mRNA was detected in 18 of 59 (31%) cases, and was significantly associated with shorter patient survival times on both univariate and multivariate analyses (p = 0.04). The presence of c-myc mRNA was also significantly associated with tumor anaplasia. While survival rates were higher for patients with low N-myc or high TrkC expression, these differences were not statistically significant. The group of patients with either moderate or severely anaplastic tumors showed only a trend towards shorter survival (p = 0.11). However, severe anaplasia alone was significantly prognostic (p = 0.002). Given the prognostic import of c-myc, we investigated 2 potential mechanisms by which its expression might be regulated: Wnt signaling and Mxi-1 mutation. Nuclear translocation of beta-catenin, a marker of Wnt pathway activation, was more common in medulloblastomas with high c-myc than in tumors overall, but the difference was not statistically significant. No Mxi-1 mutations were detected in the 22 cases examined. The association we describe between c-myc expression, tumor anaplasia, and worse clinical outcomes provides further evidence for the importance of this oncogene in medulloblastoma pathobiology.

  7. c-Myc Antagonises the Transcriptional Activity of the Androgen Receptor in Prostate Cancer Affecting Key Gene Networks.

    Science.gov (United States)

    Barfeld, Stefan J; Urbanucci, Alfonso; Itkonen, Harri M; Fazli, Ladan; Hicks, Jessica L; Thiede, Bernd; Rennie, Paul S; Yegnasubramanian, Srinivasan; DeMarzo, Angelo M; Mills, Ian G

    2017-04-01

    Prostate cancer (PCa) is the most common non-cutaneous cancer in men. The androgen receptor (AR), a ligand-activated transcription factor, constitutes the main drug target for advanced cases of the disease. However, a variety of other transcription factors and signaling networks have been shown to be altered in patients and to influence AR activity. Amongst these, the oncogenic transcription factor c-Myc has been studied extensively in multiple malignancies and elevated protein levels of c-Myc are commonly observed in PCa. Its impact on AR activity, however, remains elusive. In this study, we assessed the impact of c-Myc overexpression on AR activity and transcriptional output in a PCa cell line model and validated the antagonistic effect of c-MYC on AR-targets in patient samples. We found that c-Myc overexpression partially reprogrammed AR chromatin occupancy and was associated with altered histone marks distribution, most notably H3K4me1 and H3K27me3. We found c-Myc and the AR co-occupy a substantial number of binding sites and these exhibited enhancer-like characteristics. Interestingly, c-Myc overexpression antagonised clinically relevant AR target genes. Therefore, as an example, we validated the antagonistic relationship between c-Myc and two AR target genes, KLK3 (alias PSA, prostate specific antigen), and Glycine N-Methyltransferase (GNMT), in patient samples. Our findings provide unbiased evidence that MYC overexpression deregulates the AR transcriptional program, which is thought to be a driving force in PCa. Crown Copyright © 2017. Published by Elsevier B.V. All rights reserved.

  8. c-Myc Antagonises the Transcriptional Activity of the Androgen Receptor in Prostate Cancer Affecting Key Gene Networks

    Directory of Open Access Journals (Sweden)

    Stefan J. Barfeld

    2017-04-01

    Full Text Available Prostate cancer (PCa is the most common non-cutaneous cancer in men. The androgen receptor (AR, a ligand-activated transcription factor, constitutes the main drug target for advanced cases of the disease. However, a variety of other transcription factors and signaling networks have been shown to be altered in patients and to influence AR activity. Amongst these, the oncogenic transcription factor c-Myc has been studied extensively in multiple malignancies and elevated protein levels of c-Myc are commonly observed in PCa. Its impact on AR activity, however, remains elusive. In this study, we assessed the impact of c-Myc overexpression on AR activity and transcriptional output in a PCa cell line model and validated the antagonistic effect of c-MYC on AR-targets in patient samples. We found that c-Myc overexpression partially reprogrammed AR chromatin occupancy and was associated with altered histone marks distribution, most notably H3K4me1 and H3K27me3. We found c-Myc and the AR co-occupy a substantial number of binding sites and these exhibited enhancer-like characteristics. Interestingly, c-Myc overexpression antagonised clinically relevant AR target genes. Therefore, as an example, we validated the antagonistic relationship between c-Myc and two AR target genes, KLK3 (alias PSA, prostate specific antigen, and Glycine N-Methyltransferase (GNMT, in patient samples. Our findings provide unbiased evidence that MYC overexpression deregulates the AR transcriptional program, which is thought to be a driving force in PCa.

  9. Astroglial c-Myc overexpression predisposes mice to primary malignant gliomas

    DEFF Research Database (Denmark)

    Jensen, Niels Aagaard; Pedersen, Karen-Marie; Lihme, Frederikke

    2003-01-01

    Malignant astrocytomas are common human primary brain tumors that result from neoplastic transformation of astroglia or their progenitors. Here we show that deregulation of the c-Myc pathway in developing astroglia predisposes mice to malignant astrocytomas within 2-3 weeks of age. The genetically...... engineered murine (GEM) gliomas harbor a molecular signature resembling that of human primary glioblastoma multiforme, including up-regulation of epidermal growth factor receptor and Mdm2. The GEM gliomas seem to originate in an abnormal population of glial fibrillary acidic protein-expressing cells...... the neoplastic process, presumably by inducing the sustained growth of early astroglial cells. This is in contrast to most other transgenic studies in which c-Myc overexpression requires co-operating transgenes for rapid tumor induction....

  10. The effect of Glut1 and c-myc on prognosis in esophageal squamous cell carcinoma of Kazakh and Han patients.

    Science.gov (United States)

    Zhou, Ya-Xing; Zhou, Ke-Ming; Liu, Qian; Wang, Hui; Wang, Wen; Shi, Yi; Ma, Yu-Qing

    2018-04-09

    Glucose transporter type 1 (Glut1) plays a crucial role in cancer-specific metabolism. We explored the expression of Glut1 and c-myc, the relationship between them and the effect of Glut1, c-myc on prognosis in esophageal squamous cell carcinoma. Immunohistochemistry was used to examine the expression of Glut1 and c-myc. χ 2 test analyzes the relationship between c-myc, Glut1 and pathological parameters. Spearman correlation analyzes the relationship between c-myc and Glut1. Survival analysis was used to investigate the effect of Glut1 and c-myc on prognosis. Glut1 positivity was associated with tumor size (p C-myc positivity was associated with tumor location (p = 0.015), depth of invasion (p = 0.022) and lymph node metastasis (p = 0.035). There was a positive correlation between c-myc and Glut1 (r = 0.321). Patients with Glut1 c-myc co-expression had poorer prognosis. Inhibiting Glut1 c-myc co-expression may improve the prognosis of esophageal squamous cell carcinoma.

  11. Catch statistics for belugas in West Greenland 1862 to 1999

    Directory of Open Access Journals (Sweden)

    MP Heide-Jørgensen

    2002-07-01

    Full Text Available Information and statistics including trade statistics on catches of white whales or belugas (Delphinapterus leucas in West Greenland since 1862 are presented. The period before 1952 was dominated by large catches south of 66o N that peaked with 1,380 reported kills in 1922. Catch levels in the past five decades are evaluated on the basis of official catch statistics, trade in mattak (whale skin, sampling of jaws and reports from local residents and other observers. Options are given for corrections of catch statistics based upon auxiliary statistics on trade of mattak, catches in previous decades for areas without reporting and on likely levels of loss rates in different hunting operations. The fractions of the reported catches that are caused by ice entrapments of whales are estimated. During 1954-1999 total reported catches ranged from 216 to 1,874 and they peaked around 1970. Correcting for underreporting and killed-but-lost whales increases the catch reports by 42% on average for 1954-1998. If the whales killed in ice entrapments are removed then the corrected catch estimate is on average 28% larger than the reported catches.

  12. Targeting c-Myc: JQ1 as a promising option for c-Myc-amplified esophageal squamous cell carcinoma.

    Science.gov (United States)

    Wang, Jingyuan; Liu, Zhentao; Wang, Ziqi; Wang, Shubin; Chen, Zuhua; Li, Zhongwu; Zhang, Mengqi; Zou, Jianling; Dong, Bin; Gao, Jing; Shen, Lin

    2018-04-10

    c-Myc amplification-induced cell cycle dysregulation is a common cause for esophageal squamous cell carcinoma (ESCC), but no approved targeted drug is available so far. The bromodomain inhibitor JQ1, which targets c-Myc, exerts anti-tumor activity in multiple cancers. However, the role of JQ1 in ESCC remains unknown. In this study, we reported that JQ1 had potent anti-proliferative effects on ESCC cells in both time- and dose-dependent manners by inducing cell cycle arrest at G1 phase, cell apoptosis, and the mesenchymal-epithelial transition. Follow-up studies revealed that both c-Myc/cyclin/Rb and PI3K/AKT signaling pathways were inactivated by JQ1, as indicated by the downregulation of c-Myc, cyclin A/E, and phosphorylated Rb, AKT and S6. Tumor suppression induced by JQ1 in c-Myc amplified or highly expressed xenografts was higher than that in xenografts with low expression, suggesting its potential role in prediction. In conclusion, targeting c-Myc by JQ1 could cause significant tumor suppression in ESCC both in vitro and in vivo. Also, c-Myc amplification or high expression might serve as a potential biomarker and provide a promising therapeutic option for ESCC. Copyright © 2018 Elsevier B.V. All rights reserved.

  13. Vision Algorithms Catch Defects in Screen Displays

    Science.gov (United States)

    2014-01-01

    Andrew Watson, a senior scientist at Ames Research Center, developed a tool called the Spatial Standard Observer (SSO), which models human vision for use in robotic applications. Redmond, Washington-based Radiant Zemax LLC licensed the technology from NASA and combined it with its imaging colorimeter system, creating a powerful tool that high-volume manufacturers of flat-panel displays use to catch defects in screens.

  14. c-Myc oncogene expression in selected odontogenic cysts and tumors: An immunohistochemical study

    Science.gov (United States)

    Moosvi, Zama; Rekha, K

    2013-01-01

    Aim: To investigate the role of c-Myc oncogene in selected odontogenic cysts and tumors. Materials and Methods: Ten cases each of ameloblastoma, adenomatoid odontogenic tumor (AOT), odontogenic keratocyst (OKC), dentigerous cyst, and radicular cyst were selected and primary monoclonal mouse anti-human c-Myc antibody was used in a dilution of 1: 50. Statistical Analysis was performed using Mann Whitney U test. Results: 80% positivity was observed in ameloblastoma, AOT and OKC; 50% positivity in radicular cyst and 20% positivity in dentigerous cyst. Comparison of c-Myc expression between ameloblastoma and AOT did not reveal significant results. Similarly, no statistical significance was observed when results of OKC were compared with ameloblastoma and AOT. In contrast, significant differences were seen on comparison of dentigerous cyst with ameloblastoma and AOT and radicular cyst with AOT. Conclusion: From the above data we conclude that (1) Ameloblastoma and AOT have similar proliferative potential and their biologic behavior cannot possibly be attributed to it. (2) OKC has an intrinsic growth potential which is absent in other cysts and reinforces its classification as keratocystic odontogenic tumor. PMID:23798830

  15. Expression of p27 and c-Myc by immunohistochemistry in breast ductal cancers in African American women.

    Science.gov (United States)

    Khan, Farhan; Ricks-Santi, Luisel J; Zafar, Rabia; Kanaan, Yasmine; Naab, Tammey

    2018-06-01

    Proteins p27 and c-Myc are both key players in the cell cycle. While p27, a tumor suppressor, inhibits progression from G1 to S phase, c-Myc, a proto-oncogene, plays a key role in cell cycle regulation and apoptosis. The objective of our study was to determine the association between expression of c-Myc and the loss of p27 by immunohistochemistry (IHC) in the four major subtypes of breast cancer (BC) (Luminal A, Luminal B, HER2, and Triple Negative) and with other clinicopathological factors in a population of 202 African-American (AA) women. Tissue microarrays (TMAs) were constructed from FFPE tumor blocks from primary ductal breast carcinomas in 202 AA women. Five micrometer sections were stained with a mouse monoclonal antibody against p27 and a rabbit monoclonal antibody against c-Myc. The sections were evaluated for intensity of nuclear reactivity (1-3) and percentage of reactive cells; an H-score was derived from the product of these measurements. Loss of p27 expression and c-Myc overexpression showed statistical significance with ER negative (p c-Myc expression/p27 loss and luminal A/B and Her2 overexpressing subtypes. In our study, a statistically significant association between c-Myc expression and p27 loss and the triple negative breast cancers (TNBC) was found in AA women. A recent study found that constitutive c-Myc expression is associated with inactivation of the axin 1 tumor suppressor gene. p27 inhibits cyclin dependent kinase2/cyclin A/E complex formation. Axin 1 and CDK inhibitors may represent possible therapeutic targets for TNBC. Copyright © 2018 Elsevier Inc. All rights reserved.

  16. Acidosis Decreases c-Myc Oncogene Expression in Human Lymphoma Cells: A Role for the Proton-Sensing G Protein-Coupled Receptor TDAG8

    Directory of Open Access Journals (Sweden)

    Zhigang Li

    2013-10-01

    Full Text Available Acidosis is a biochemical hallmark of the tumor microenvironment. Here, we report that acute acidosis decreases c-Myc oncogene expression in U937 human lymphoma cells. The level of c-Myc transcripts, but not mRNA or protein stability, contributes to c-Myc protein reduction under acidosis. The pH-sensing receptor TDAG8 (GPR65 is involved in acidosis-induced c-Myc downregulation. TDAG8 is expressed in U937 lymphoma cells, and the overexpression or knockdown of TDAG8 further decreases or partially rescues c-Myc expression, respectively. Acidic pH alone is insufficient to reduce c-Myc expression, as it does not decrease c-Myc in H1299 lung cancer cells expressing very low levels of pH-sensing G protein-coupled receptors (GPCRs. Instead, c-Myc is slightly increased by acidosis in H1299 cells, but this increase is completely inhibited by ectopic overexpression of TDAG8. Interestingly, TDAG8 expression is decreased by more than 50% in human lymphoma samples in comparison to non-tumorous lymph nodes and spleens, suggesting a potential tumor suppressor function of TDAG8 in lymphoma. Collectively, our results identify a novel mechanism of c-Myc regulation by acidosis in the tumor microenvironment and indicate that modulation of TDAG8 and related pH-sensing receptor pathways may be exploited as a new approach to inhibit Myc expression.

  17. Impact of catch crop mixtures and soils on microbial diversity and nitrogen cycling communities in agroecosystems

    Science.gov (United States)

    Burbano, Claudia S.; Große, Julia; Hurek, Thomas; Reinhold-Hurek, Barbara

    2017-04-01

    In light of the projected world's population growth, food supplies will necessary have to increase. Soils are an essential component for achieving this expansion and its quality and fertility are crucial for bio-economic productivity. Catch crops can be an option to preserve or even improve soil productivity because of their effect on soil fertility and health. A long-term field experiment of the CATCHY project (Catch-cropping as an agrarian tool for continuing soil health and yield-increase) with two contrasting crop rotations was established in two different locations in Northern and Southern Germany. Single catch crops (white mustard, Egyptian clover, phacelia and bristle oat), catch crop mixtures (a mixture of the above and a commercial mixture) and main crops (wheat and maize) have been grown. To investigate how catch crops can affect the microbial diversity and particularly the microbial nitrogen cycling communities, we are studying first the short-term effect of different catch crop mixtures on the microbiomes associated with soils and roots. We compared these microbiomes with wheat plants, representing the microbial community before a catch crop treatment. Roots, rhizosphere and bulk soils were collected from representative samples of wheat plants from one field. The same compartments were also sampled from one fallow treatment and three catch crops variants from three fields each. The variants consisted of white mustard and the two catch crop mixtures. All fields were sampled by triplicate. Quantitative analyses were carried out by qPCR based on key functional marker genes for mineralization (ureC), nitrification (amoA), dissimilatory nitrate and nitrite reduction to ammonium -DNRA- (nrfA), denitrification (nirK, nirS, nosZ), and nitrogen fixation (nifH). These genes were targeted at the DNA and RNA level for the characterization of the microbial population and the actual transcription activity, respectively. We detected the presence and activity of

  18. Vision Based Tracker for Dart-Catching Robot

    OpenAIRE

    Linderoth, Magnus; Robertsson, Anders; Åström, Karl; Johansson, Rolf

    2009-01-01

    This paper describes how high-speed computer vision can be used in a motion control application. The specific application investigated is a dart catching robot. Computer vision is used to detect a flying dart and a filtering algorithm predicts its future trajectory. This will give data to a robot controller allowing it to catch the dart. The performance of the implemented components indicates that the dart catching application can be made to work well. Conclusions are also made about what fea...

  19. Catch tanks inhibitor addition 200-East and 200-West Areas

    International Nuclear Information System (INIS)

    Palit, A.N.

    1996-01-01

    Reported is the study of 11 catch tanks in the 200-East Area and the 7 catch tanks in the 200-West Area listed as active. The location, capacity, material of construction, annual total accumulation, annual rain intrusion, waste transfer rate, and access for chemical injection in these tanks are documented. The present and future utilization and isolation plans for the catch tanks are established

  20. Ethical Concern in Joseph Heller’s Catch-22

    Institute of Scientific and Technical Information of China (English)

    XIAN Yu-jing

    2015-01-01

    In Catch-22, Joseph Heller shows his ethical concern behind absurdity, which is manifested from his attitude toward individualism and utilitarianism, religious belief, sexual promiscuity, interpersonal relationship, etc.

  1. Transcriptional regulation of BRD7 expression by Sp1 and c-Myc

    Directory of Open Access Journals (Sweden)

    Li Shufang

    2008-12-01

    Full Text Available Abstract Background Bromodomain is an evolutionally conserved domain that is found in proteins strongly implicated in signal-dependent transcriptional regulation. Genetic alterations of bromodomain genes contributed to the development of many human cancers and other disorders. BRD7 is a recently identified bromodomain gene. It plays a critical role in cellular growth, cell cycle progression, and signal-dependent gene expression. Previous studies showed that BRD7 gene exhibited much higher-level of mRNA expression in normal nasopharyngeal epithelia than in nasopharyngeal carcinoma (NPC biopsies and cell lines. However, little is known about its transcriptional regulation. In this study, we explored the transcriptional regulation of BRD7 gene. Method Potential binding sites of transcription factors within the promoter region of BRD7 gene were predicted with MatInspector Professional http://genomatix.de/cgi-bin/matinspector_prof/mat_fam.pl. Mutation construct methods and luciferase assays were performed to define the minimal promoter of BRD7 gene. RT-PCR and western blot assays were used to detect the endogenous expression of transcription factor Sp1, c-Myc and E2F6 in all cell lines used in this study. Electrophoretic mobility shift assays (EMSA and Chromatin immunoprecipitation (ChIP were used to detect the direct transcription factors that are responsible for the promoter activity of BRD7 gene. DNA vector-based siRNA technology and cell transfection methods were employed to establish clone pools that stably expresses SiRNA against c-Myc expression in nasopharyngeal carcinoma 5-8F cells. Real-time PCR was used to detect mRNA expression of BRD7 gene in 5-8F/Si-c-Myc cells. Results We defined the minimal promoter of BRD7 gene in a 55-bp region (from -266 to -212bp, and identified that its promoter activity is inversely related to c-Myc expression. Sp1 binds to the Sp1/Myc-Max overlapping site of BRD7 minimal promoter, and slightly positively

  2. Globalization, nation-state and catching up

    Directory of Open Access Journals (Sweden)

    Luiz Carlos Bresser-Pereira

    2008-12-01

    Full Text Available Globalization and nation-states are not in contradiction, since globalization is the present stage of capitalist development, and the nation-state is the territorial political unit that organizes the space and population in the capitalist system. Since the 1980s, Global Capitalism constitutes the economic system characterized by the opening of all national markets and a fierce competition between nation-states. Developing countries tend to catch up, while rich countries try to neutralize such competitive effort, using globalism as an ideology, and conventional orthodoxy as a strategy. Middle-income countries that are catching up in the realm of globalization are the ones that count with a national development strategy. This is broadly the case of the dynamic Asian countries. In contrast, Latin American countries have no longer their own strategy, and grow less. To add data to the argument, the author conducts an econometric test comparing these two groups of countries, and three variables: the rate of investment, the current account deficit or surplus that would indicate or not a competitive exchange rate, and public deficit.

  3. A proteomic study of cMyc improvement of CHO culture

    Directory of Open Access Journals (Sweden)

    Dunn Michael J

    2010-03-01

    Full Text Available Abstract Background The biopharmaceutical industry requires cell lines to have an optimal proliferation rate and a high integral viable cell number resulting in a maximum volumetric recombinant protein product titre. Nutrient feeding has been shown to boost cell number and productivity in fed-batch culture, but cell line engineering is another route one may take to increase these parameters in the bioreactor. The use of CHO-K1 cells with a c-myc plasmid allowing for over-expressing c-Myc (designated cMycCHO gives a higher integral viable cell number. In this study the differential protein expression in cMycCHO is investigated using two-dimensional gel electrophoresis (2-DE followed by image analysis to determine the extent of the effect c-Myc has on the cell and the proteins involved to give the new phenotype. Results Over 100 proteins that were differentially expressed in cMycCHO cells were detected with high statistical confidence, of which 41 were subsequently identified by tandem mass spectrometry (LC-MS/MS. Further analysis revealed proteins involved in a variety of pathways. Some examples of changes in protein expression include: an increase in nucleolin, involved in proliferation and known to aid in stabilising anti-apoptotic protein mRNA levels, the cytoskeleton and mitochondrial morphology (vimentin, protein biosysnthesis (eIF6 and energy metabolism (ATP synthetase, and a decreased regulation of all proteins, indentified, involved in matrix and cell to cell adhesion. Conclusion These results indicate several proteins involved in proliferation and adhesion that could be useful for future approaches to improve proliferation and decrease adhesion of CHO cell lines which are difficult to adapt to suspension culture.

  4. RNA interference-mediated c-MYC inhibition prevents cell growth and decreases sensitivity to radio- and chemotherapy in childhood medulloblastoma cells

    International Nuclear Information System (INIS)

    Bueren, André O von; Shalaby, Tarek; Oehler-Jänne, Christoph; Arnold, Lucia; Stearns, Duncan; Eberhart, Charles G; Arcaro, Alexandre; Pruschy, Martin; Grotzer, Michael A

    2009-01-01

    With current treatment strategies, nearly half of all medulloblastoma (MB) patients die from progressive tumors. Accordingly, the identification of novel therapeutic strategies remains a major goal. Deregulation of c-MYC is evident in numerous human cancers. In MB, over-expression of c-MYC has been shown to cause anaplasia and correlate with unfavorable prognosis. To study the role of c-MYC in MB biology, we down-regulated c-MYC expression by using small interfering RNA (siRNA) and investigated changes in cellular proliferation, cell cycle analysis, apoptosis, telomere maintenance, and response to ionizing radiation (IR) and chemotherapeutics in a representative panel of human MB cell lines expressing different levels of c-MYC (DAOY wild-type, DAOY transfected with the empty vector, DAOY transfected with c-MYC, D341, and D425). siRNA-mediated c-MYC down-regulation resulted in an inhibition of cellular proliferation and clonogenic growth, inhibition of G1-S phase cell cycle progression, and a decrease in human telomerase reverse transcriptase (hTERT) expression and telomerase activity. On the other hand, down-regulation of c-MYC reduced apoptosis and decreased the sensitivity of human MB cells to IR, cisplatin, and etoposide. This effect was more pronounced in DAOY cells expressing high levels of c-MYC when compared with DAOY wild-type or DAOY cells transfected with the empty vector. In human MB cells, in addition to its roles in growth and proliferation, c-MYC is also a potent inducer of apoptosis. Therefore, targeting c-MYC might be of therapeutic benefit when used sequentially with chemo- and radiotherapy rather than concomitantly

  5. RNA interference-mediated c-MYC inhibition prevents cell growth and decreases sensitivity to radio- and chemotherapy in childhood medulloblastoma cells

    Directory of Open Access Journals (Sweden)

    Arcaro Alexandre

    2009-01-01

    Full Text Available Abstract Background With current treatment strategies, nearly half of all medulloblastoma (MB patients die from progressive tumors. Accordingly, the identification of novel therapeutic strategies remains a major goal. Deregulation of c-MYC is evident in numerous human cancers. In MB, over-expression of c-MYC has been shown to cause anaplasia and correlate with unfavorable prognosis. Methods To study the role of c-MYC in MB biology, we down-regulated c-MYC expression by using small interfering RNA (siRNA and investigated changes in cellular proliferation, cell cycle analysis, apoptosis, telomere maintenance, and response to ionizing radiation (IR and chemotherapeutics in a representative panel of human MB cell lines expressing different levels of c-MYC (DAOY wild-type, DAOY transfected with the empty vector, DAOY transfected with c-MYC, D341, and D425. Results siRNA-mediated c-MYC down-regulation resulted in an inhibition of cellular proliferation and clonogenic growth, inhibition of G1-S phase cell cycle progression, and a decrease in human telomerase reverse transcriptase (hTERT expression and telomerase activity. On the other hand, down-regulation of c-MYC reduced apoptosis and decreased the sensitivity of human MB cells to IR, cisplatin, and etoposide. This effect was more pronounced in DAOY cells expressing high levels of c-MYC when compared with DAOY wild-type or DAOY cells transfected with the empty vector. Conclusion In human MB cells, in addition to its roles in growth and proliferation, c-MYC is also a potent inducer of apoptosis. Therefore, targeting c-MYC might be of therapeutic benefit when used sequentially with chemo- and radiotherapy rather than concomitantly.

  6. Localisation of lung cancer by a radiolabelled monoclonal antibody against the c-myc oncogene product

    Energy Technology Data Exchange (ETDEWEB)

    Chan, S Y.T.; Evan, G I; Ritson, A; Watson, J; Wraight, P; Sikora, K

    1986-11-01

    A set of mouse monoclonal antibodies against the c-myc oncogene product, a 62,000 dalton nuclear binding protein involved in cell cycle control, has been constructed by immunisation with synthetic peptide fragments. One such antibody, CT14, was radiolabelled with /sup 131/I and administered to 20 patients with different malignant diseases. Good tumour localisation was observed in 12 out of 14 patients with primary bronchial carcinoma but not in patients with pulmonary metastases from primary tumours elsewhere. Successfully localised tumours were all 3 cm or more in diameter. Monoclonal antibodies against oncogene products may provide novel selective tools for the diagnosis and therapy of cancer.

  7. EFFECT OF STENT ABSORBED c-myc ANTISENSE OLIGODEOXYNUCLEOTIDE ON SMOOTH MUSCLE CELLS APOPTOSIS IN RABBIT CAROTID ARTERY

    Institute of Scientific and Technical Information of China (English)

    张新霞; 崔长琮; 李江; 崔翰斌; 徐仓宝; 朱参战

    2002-01-01

    Objective To investigate the effect of gelatin coated Platinium-Iridium stent absorbed c-myc antisense oligodeoxynucleotide (ASODN) on smooth muscle cells apoptosis in a normal rabbit carotid arteries. Methods Gelatin coated Platinium-Iridium stents were implanted in the right carotid arteries of 32 rabbits under vision. Animals were randomly divided into control group and treated group receiving c-myc ASODN (n=16, respectively). On 7, 14, 30 and 90 days following the stenting procedure ,morphometry for caculation of neointimal area and mean neointimal thickness were performed.The expression of c-myc protein was detected by immunohistochemical method. Apoptotic smooth muscle cells was detected by terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL). Results At 7 and 14 days after stenting,there were no detectable apoptotic cells in both groups. The apoptotic cells occurred in the neointima 30 and 90 days after stenting, and the number of apoptotic cells at 30 days were less [4.50±1.29 vs 25.75±1.89 (number/0.1mm2)] than that at 90 days [13.50±1.91 vs 41.50±6.46 (number/0.1mm2)]. Meanwhile c-myc ASODN induced more apoptotic cells than the control group(P<0.0001). c-myc protein expression was weak positive or negative in treated group and positive in control group.Conclusion c-myc ASODN can induce smooth muscle cells apoptosis after stenting in normal rabbit carotid arteries,and it can be used to prevent in-stent restenosis.

  8. TATA-binding protein and the retinoblastoma gene product bind to overlapping epitopes on c-Myc and adenovirus E1A protein

    NARCIS (Netherlands)

    Hateboer, G.; Timmers, H.T.M.; Rustgi, A.K.; Billaud, Marc; Veer, L.J. Van 't; Bernards, R.A.

    1993-01-01

    Using a protein binding assay, we show that the amino-teminal 204 amino acids of the c-Myc protein interact di y with a key component of the basal p tdon factor TFID, the TATA box-binding protein (TBP). Essentialy the same region of the c-Myc protein alo binds the product of the retinoblatoma

  9. Assessment of selenium mineralization and availability from catch crops

    DEFF Research Database (Denmark)

    Stavridou, Eleftheria; Thorup-Kristensen, Kristian; Young, S.D.

    2011-01-01

    Selenium (Se) release from four plant species (Indian mustard, fodder radish, Italian ryegrass and hairy vetch) was measured under controlled leaching conditions and in a pot incubation experiment as part of a study of the potential for using these plant species as Se catch crops. Catch crops may...

  10. Seasonal abundance, distribution, and catch per unit effort using gill ...

    African Journals Online (AJOL)

    Catch per unit effort was obtained for the fish of the Sundays .... Methods. Catch per unit effort (numbers and weight/net) of fish in the estuary was obtained from 55 .... Table 1 CPUE (number and mass) of fish caught monthly using gill-net over 12·h periods with 55 nettings at .... The abundance of some other species may.

  11. Quantifying commercial catch and effort of monkfish Lophius ...

    African Journals Online (AJOL)

    Catch-per-unit-effort (cpue) data of vessels targeting monkfish and sole (the two ... analysed using two different methods to construct indices of abundance. ... in Namibia to all tail-weight classes is not appropriate for the current fishery and needs ... Keywords: catch per unit effort, Generalized Linear Model, Lophius vaillanti, ...

  12. Assessment of catches in shore angling competitions from the ...

    African Journals Online (AJOL)

    By mass, most of the catch was made up of R. annulatus (24 %), D. c. chrysonota (19 %), Carcharius taurus (16 %) and A. japonicus (13 %). These species constituted the most important species during each year of the study period. Mean annual catch per unit effort (CPUE) by number and mass has decreased slightly over ...

  13. Comparative study on the human landing catch and light trap ...

    African Journals Online (AJOL)

    As part of a longitudinal study on the epidemiology of filariasis, this study was carried out to test a hypothesis that three light traps catch about the same number of mosquitoes as a team of two Human Landing Catch collectors. The comparison was done to analyze possible differences between the two techniques in the ...

  14. Nutritionally-Induced Catch-Up Growth

    Directory of Open Access Journals (Sweden)

    Galia Gat-Yablonski

    2015-01-01

    Full Text Available Malnutrition is considered a leading cause of growth attenuation in children. When food is replenished, spontaneous catch-up (CU growth usually occurs, bringing the child back to its original growth trajectory. However, in some cases, the CU growth is not complete, leading to a permanent growth deficit. This review summarizes our current knowledge regarding the mechanism regulating nutrition and growth, including systemic factors, such as insulin, growth hormone, insulin- like growth factor-1, vitamin D, fibroblast growth factor-21, etc., and local mechanisms, including autophagy, as well as regulators of transcription, protein synthesis, miRNAs and epigenetics. Studying the molecular mechanisms regulating CU growth may lead to the establishment of better nutritional and therapeutic regimens for more effective CU growth in children with malnutrition and growth abnormalities. It will be fascinating to follow this research in the coming years and to translate the knowledge gained to clinical benefit.

  15. NC CATCH: Advancing Public Health Analytics.

    Science.gov (United States)

    Studnicki, James; Fisher, John W; Eichelberger, Christopher; Bridger, Colleen; Angelon-Gaetz, Kim; Nelson, Debi

    2010-01-01

    The North Carolina Comprehensive Assessment for Tracking Community Health (NC CATCH) is a Web-based analytical system deployed to local public health units and their community partners. The system has the following characteristics: flexible, powerful online analytic processing (OLAP) interface; multiple sources of multidimensional, event-level data fully conformed to common definitions in a data warehouse structure; enabled utilization of available decision support software tools; analytic capabilities distributed and optimized locally with centralized technical infrastructure; two levels of access differentiated by the user (anonymous versus registered) and by the analytical flexibility (Community Profile versus Design Phase); and, an emphasis on user training and feedback. The ability of local public health units to engage in outcomes-based performance measurement will be influenced by continuing access to event-level data, developments in evidence-based practice for improving population health, and the application of information technology-based analytic tools and methods.

  16. Social Cultural Data - Social Impacts of Catch Shares in the West Coast Groundfish Fishery

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Catch shares are one method of catch allocation utilized by fisheries managers in the United States West Coast groundfish fishery. Catch share management results in...

  17. Hsa-let-7a functions as a tumor suppressor in renal cell carcinoma cell lines by targeting c-myc

    Energy Technology Data Exchange (ETDEWEB)

    Liu, Yongchao; Yin, Bingde; Zhang, Changcun; Zhou, Libin [Department of Urology, Shanghai First People' s Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200080 (China); Fan, Jie, E-mail: jief67@sina.com [Department of Urology, Shanghai First People' s Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200080 (China)

    2012-01-06

    Highlights: Black-Right-Pointing-Pointer This study is the first to test the let-7a/c-myc loop in renal cell carcinoma cell lines. Black-Right-Pointing-Pointer Let-7a down-regulated c-myc in three renal cell carcinoma cell lines. Black-Right-Pointing-Pointer c-myc target genes were down-regulated because of the let-7a-mediated down-regulation of c-myc. Black-Right-Pointing-Pointer The let-7a/c-myc loop has a significant function in renal cell carcinoma cell lines. -- Abstract: Widespread functions of the c-myc pathway play a crucial role in renal cell carcinoma (RCC) carcinogenesis. Thus, we evaluated the connection between proto-oncogenic c-myc and anti-neoplastic hsa-let-7a (let-7a) in RCC cell lines. The levels of c-myc and let-7a in 3 RCC cell lines (769P, Caki-1 and 786O) were measured after transfecting the cells with let-7a mimics or a negative control. The change in c-myc protein level was confirmed by Western blot. The anti-neoplastic function of let-7a was evaluated using cell counting kit-8 (CCK-8) for proliferation analysis and cell flow cytometry for cell cycle analysis. The changes of downstream targets of c-myc were measured using reverse transcription quantitative real-time PCR (qRT-PCR). Our results suggest for the first time that let-7a acts as a tumor suppressor in RCC cell lines by down-regulating c-myc and c-myc target genes such as proliferating cell nuclear antigen (PCNA), cyclin D1 (CCND1) and the miR17-92 cluster, which is accompanied by proliferation inhibition and cell cycle arrest.

  18. Insertion of the LINE-1 element in the C-MYC gene and immunoreactivity of C-MYC, p53, p21 and p27 proteins in different morphological patterns of the canine TVT

    Directory of Open Access Journals (Sweden)

    C.R.O. Lima

    2016-06-01

    Full Text Available ABSTRACT The canine transmissible venereal tumor (TVT affects the external genitalia of dogs by the natural transplant of viable tumor cells. Thus, this research aimed to diagnose and characterize TVT morphological patterns, identify the insertion of the LINE-1 element in C-MYC gene, by means of the polymerase chain reaction (PCR, and evaluate the immunohistochemical expression of C-MYC, p53, p21 and p27 proteins. The relationship between C-MYC and p53 proteins and their interference on the expression of p21 and p27 were also studied. For that, 20 samples of naturally occurring TVT were used, subjected to cytopathological, histopathological and immunohistochemical analysis, and to molecular diagnosis of neoplasia. The increased tissue expression and the correlation among C-MYC, p53, p21 and p27 proteins indicate reduction and/or loss of their functionality in the TVT microenvironment, with consequent apoptotic suppression, maintenance of cell growth and progression of neoplasia.

  19. Yields of Selected Catch Crops in Dry Conditions

    Directory of Open Access Journals (Sweden)

    Martina Handlířová

    2016-01-01

    Full Text Available Catch crops mainly reduce soil erosion and leaching of nutrients as well as enrich the soil organic matter. The aim of this research is to evaluate the yields of catch crops of Sinapis alba, Phacelia tanacetifolia, Fagopyrum esculentum, Carthamus tinctorius and Secale cereale v. multicaule, and thus determine the possible applicability of catch crops in areas with high average annual temperature and low precipitation totals. The small-plot field experiment was performed on clay-loam gleyic fluvisol at the Field Experimental Station in Žabčice, Southern Moravia, Czech Republic, within the period of 2006-2014. The catch crops were set up after winter wheat in mid-August. The results have shown a statistically significant difference among different catch crops in yield of dry matter and even among years. The yield of catch crops is mainly dependent on a sufficient supply of water in the soil and the appropriate amount and distribution of rainfall over the growing season. Sinapis alba and Phacelia tanacetifolia regularly reached the highest yields. High yields were also achieved with Fagopyrum esculentum. Due to the method of crop rotation in the Czech Republic, with a predominance of Brassica napus var. napus, it is inappropriate to include Sinapis alba. It is the best to grow Phacelia tanacetifolia and even Fagopyrum esculentum, or a mixture thereof, depending on the use of catch crops.

  20. SirT1 confers hypoxia-induced radioresistance via the modulation of c-Myc stabilization on hepatoma cells

    International Nuclear Information System (INIS)

    Xie Yuexia; Zhang Jianghong; Shao Chunlin; Xu Yanwu

    2012-01-01

    Intratumoral hypoxia is an important contributory factor to tumor cell resistance to radiotherapy. SirT1, a nicotinamide adenine dinucleotide (NAD + )-dependent histone/protein deacetylase, has been linked to the decrease of radiation-induced DNA damage and seems to be critical for cancer therapy. The purpose of this study was to investigate the role of SirT1 in hypoxia-induced radiation response on hepatoma cells. It was found that the administration with resveratrol, a putative SirT1 activator, enhanced the resistance of HepG2 cells against radiation-induced DNA damage of MN formation under hypoxia condition; while nicotinamide, a well-known SirT1 inhibitor, sensitized this radiation damage. Nevertheless, pretreatment of cells with 10058-F4, a specific inhibitor of c-Myc, almost eliminated the nicotinamide-induced radiosensitive effect. Further studies revealed that resveratrol inhibited c-Myc protein accumulation via up-regulation of SirT1 expression and deacetylase activity, and this loss of c-Myc protein was abolished by inhibiting its degradation in the presence of MG132, a potent inhibitor of proteasome. In contrast, nicotinamide attenuated c-Myc protein degradation induced by radiation under hypoxia through inhibition of SirT1 deacetylase activity. Our findings suggest that SirT1 could serve as a novel potent target of radiation-induced DNA damage and thus as a potential strategy to advance the efficiency of radiation therapy in hepatoma entities. (author)

  1. Effects of c-myc oncogene modulation on differentiation of human small cell lung carcinoma cell lines

    NARCIS (Netherlands)

    Van Waardenburg, RCAM; Meijer, C; Pinto-Sietsma, SJ; De Vries, EGE; Timens, W; Mulder, NM

    1998-01-01

    Amplification and over-expression of oncogenes of the myc family are related to the prognosis of certain solid tumors such as small cell lung cancer (SCLC). For SCLC, c-myc is the oncogene most consistently found to correlate with the end stage behaviour of the tumour, in particular with survival

  2. Cdx1 and c-Myc foster the initiation of transdifferentiation of the normal esophageal squamous epithelium toward Barrett's esophagus.

    Directory of Open Access Journals (Sweden)

    Douglas B Stairs

    Full Text Available Barrett's esophagus is a premalignant condition whereby the normal stratified squamous esophageal epithelium undergoes a transdifferentiation program resulting in a simple columnar epithelium reminiscent of the small intestine. These changes are typically associated with the stratified squamous epithelium chronically exposed to acid and bile salts as a result of gastroesophageal reflux disease (GERD. Despite this well-defined epidemiologic association between acid reflux and Barrett's esophagus, the genetic changes that induce this transdifferentiation process in esophageal keratinocytes have remained undefined.To begin to identify the genetic changes responsible for transdifferentiaiton in Barrett's esophagus, we performed a microarray analysis of normal esophageal, Barrett's esophagus and small intestinal biopsy specimens to identify candidate signaling pathways and transcription factors that may be involved. Through this screen we identified the Cdx1 homeodomain transcription factor and the c-myc pathway as possible candidates. Cdx1 and c-myc were then tested for their ability to induce transdifferentiation in immortalized human esophageal keratinocytes using organotypic culturing methods. Analyses of these cultures reveal that c-myc and cdx1 cooperate to induce mucin production and changes in keratin expression that are observed in the epithelium of Barrett's esophagus.These data demonstrate the ability of Cdx1 and c-myc to initiate the earliest stages of transdifferentiation of esophageal keratinocytes toward a cell fate characteristic of Barrett's esophagus.

  3. Expression of c-myc and c-fos and binding sites for estradiol and progesterone in human pituitary tumors.

    Science.gov (United States)

    Machiavelli, G A; Rivolta, C M; Artese, R; Basso, A; Burdman, J A

    1998-12-01

    We studied the concentration of mRNA from the oncogenes c-myc and c-fos in human pituitary adenomas by Northern blot hybridization (35 somatotrophinomas, 9 prolactinomas, 21 nonsecreting and 3 adrenocorticotrophinomas). The concentration of estrogens and progesterone receptors was also investigated. The levels of c-myc and c-fos mRNA was higher in nonsecreting tumors which were generally the largest and had a higher percentage of recurrence after surgery than the other groups. High concentration of estrogen receptors was observed in tumors derived from cells which are normally the target of this hormone, mainly prolactinomas. They were also present in somatotrophic and nonsecreting adenomas, related to the presence of prolactin or gonadotrophin cells in these tumors. The presence of estrogen receptors indicates that the tumor cells maintain their differentiation and a good prognosis as is the case for prolactinomas. We did not find any relationship between estrogen receptors and the concentration of c-myc and c-fos oncogenes. Larger adenomas (mainly nonsecreting) had higher levels of c-myc and c-fos mRNA than the other tumors and they had an important percentage of recurrence after surgery. It is clear that tumor size is related to the outcome after surgery and that nonsecreting adenomas are usually large because of the late diagnosis. However two large somatotrophinomas with extrasellar expansion also had overexpression of both oncogenes and both relapsed after surgery.

  4. Overexpression of c-myc is associated with adverse clinical features and worse overall survival in multiple myeloma

    DEFF Research Database (Denmark)

    Szabo, Agoston Gyula; Gang, Anne Ortved; Pedersen, Mette Ølgod

    2016-01-01

    The role of c-myc in multiple myeloma (MM) is controversial. We conducted a retrospective study of 117 patients with MM diagnosed between 2004 and 2010 at Herlev Hospital. Immunohistochemistry (IHC) and fluorescent in situ hybridization (FISH) were performed on tissue microarrays (TMAs) made from...

  5. c-Myc Enhances Sonic Hedgehog-Induced Medulloblastoma Formation from Nestin-Expressing Neural Progenitors in Mice

    Directory of Open Access Journals (Sweden)

    Ganesh Rao

    2003-05-01

    Full Text Available Medulloblastomas are malignant brain tumors that arise in the cerebella of children. The presumed cellsof-origin are undifferentiated precursors of granule neurons that occupy the external granule layer (EGL of the developing cerebellum. The overexpression of proteins that normally stimulate proliferation of neural progenitor cells may initiate medulloblastoma formation. Two known mitogens for neural progenitors are the c-Myc oncoprotein and Sonic hedgehog (Shh, a crucial determinant of embryonic pattern formation in the central nervous system. We modeled the ability of c-Myc and Shh to induce medulloblastoma in mice using the RCAS/tv-a system, which allows postnatal gene transfer and expression in a cell type-specific manner. We targeted the expression of Shh and c-Myc to nestin-expressing neural progenitor cells by injecting replication-competent ALV splice acceptor (RCAS vectors into the cerebella of newborn mice. Following injection with RCAS-Shh alone, 3/32 (9% mice developed medulloblastomas and 5/32 showed multifocal hyperproliferation of the EGL, possibly a precursor stage of medulloblastoma. Following injection with RCAS-Shh plus RCAS-Myc, 9/39 (23% mice developed medulloblastomas. We conclude that nestin-expressing neural progenitors, present in the cerebellum at birth, can act as the cells-of-origin for medulloblastoma, and that c-Myc cooperates with Shh to enhance tumorigenicity.

  6. XPC Promotes Pluripotency of Human Dental Pulp Cells through Regulation of Oct-4/Sox2/c-Myc

    Directory of Open Access Journals (Sweden)

    Lu Liu

    2016-01-01

    Full Text Available Introduction. Xeroderma pigmentosum group C (XPC, essential component of multisubunit stem cell coactivator complex (SCC, functions as the critical factor modulating pluripotency and genome integrity through interaction with Oct-4/Sox2. However, its specific role in regulating pluripotency and multilineage differentiation of human dental pulp cells (DPCs remains unknown. Methods. To elucidate the functional role XPC played in pluripotency and multilineage differentiation of DPCs, expressions of XPC in DPCs with long-term culture were examined by real-time PCR and western blot. DPCs were transfected with lentiviral-mediated human XPC gene; then transfection rate was investigated by real-time PCR and western blot. Cell cycle, apoptosis, proliferation, senescence, multilineage differentiation, and expression of Oct-4/Sox2/c-Myc in transfected DPCs were examined. Results. XPC, Oct-4, Sox2, and c-Myc were downregulated at P7 compared with P3 in DPCs with long-term culture. XPC genes were upregulated in DPCs at P2 after transfection and maintained high expression level at P3 and P7. Cell proliferation, PI value, and telomerase activity were enhanced, whereas apoptosis was suppressed in transfected DPCs. Oct-4/Sox2/c-Myc were significantly upregulated, and multilineage differentiation in DPCs with XPC overexpression was enhanced after transfection. Conclusions. XPC plays an essential role in the modulation of pluripotency and multilineage differentiation of DPCs through regulation of Oct-4/Sox2/c-Myc.

  7. XPC Promotes Pluripotency of Human Dental Pulp Cells through Regulation of Oct-4/Sox2/c-Myc.

    Science.gov (United States)

    Liu, Lu; Peng, Zhengjun; Xu, Zhezhen; Wei, Xi

    2016-01-01

    Introduction. Xeroderma pigmentosum group C (XPC), essential component of multisubunit stem cell coactivator complex (SCC), functions as the critical factor modulating pluripotency and genome integrity through interaction with Oct-4/Sox2. However, its specific role in regulating pluripotency and multilineage differentiation of human dental pulp cells (DPCs) remains unknown. Methods. To elucidate the functional role XPC played in pluripotency and multilineage differentiation of DPCs, expressions of XPC in DPCs with long-term culture were examined by real-time PCR and western blot. DPCs were transfected with lentiviral-mediated human XPC gene; then transfection rate was investigated by real-time PCR and western blot. Cell cycle, apoptosis, proliferation, senescence, multilineage differentiation, and expression of Oct-4/Sox2/c-Myc in transfected DPCs were examined. Results. XPC, Oct-4, Sox2, and c-Myc were downregulated at P7 compared with P3 in DPCs with long-term culture. XPC genes were upregulated in DPCs at P2 after transfection and maintained high expression level at P3 and P7. Cell proliferation, PI value, and telomerase activity were enhanced, whereas apoptosis was suppressed in transfected DPCs. Oct-4/Sox2/c-Myc were significantly upregulated, and multilineage differentiation in DPCs with XPC overexpression was enhanced after transfection. Conclusions. XPC plays an essential role in the modulation of pluripotency and multilineage differentiation of DPCs through regulation of Oct-4/Sox2/c-Myc.

  8. c-Myc affects mRNA translation, cell proliferation and progenitor cell function in the mammary gland

    Directory of Open Access Journals (Sweden)

    Trumpp Andreas

    2009-09-01

    Full Text Available Abstract Background The oncoprotein c-Myc has been intensely studied in breast cancer and mouse mammary tumor models, but relatively little is known about the normal physiological role of c-Myc in the mammary gland. Here we investigated functions of c-Myc during mouse mammary gland development using a conditional knockout approach. Results Generation of c-mycfl/fl mice carrying the mammary gland-specific WAPiCre transgene resulted in c-Myc loss in alveolar epithelial cells starting in mid-pregnancy. Three major phenotypes were observed in glands of mutant mice. First, c-Myc-deficient alveolar cells had a slower proliferative response at the start of pregnancy, causing a delay but not a block of alveolar development. Second, while milk composition was comparable between wild type and mutant animals, milk production was reduced in mutant glands, leading to slower pup weight-gain. Electron microscopy and polysome fractionation revealed a general decrease in translational efficiency. Furthermore, analysis of mRNA distribution along the polysome gradient demonstrated that this effect was specific for mRNAs whose protein products are involved in milk synthesis. Moreover, quantitative reverse transcription-polymerase chain reaction analysis revealed decreased levels of ribosomal RNAs and ribosomal protein-encoding mRNAs in mutant glands. Third, using the mammary transplantation technique to functionally identify alveolar progenitor cells, we observed that the mutant epithelium has a reduced ability to repopulate the gland when transplanted into NOD/SCID recipients. Conclusion We have demonstrated that c-Myc plays multiple roles in the mouse mammary gland during pregnancy and lactation. c-Myc loss delayed, but did not block proliferation and differentiation in pregnancy. During lactation, lower levels of ribosomal RNAs and proteins were present and translation was generally decreased in mutant glands. Finally, the transplantation studies suggest a role

  9. The Max b-HLH-LZ can transduce into cells and inhibit c-Myc transcriptional activities.

    Directory of Open Access Journals (Sweden)

    Martin Montagne

    Full Text Available The inhibition of the functions of c-Myc (endogenous and oncogenic was recently shown to provide a spectacular therapeutic index in cancer mouse models, with complete tumor regression and minimal side-effects in normal tissues. This was achieved by the systemic and conditional expression of omomyc, the cDNA of a designed mutant of the b-HLH-LZ of c-Myc named Omomyc. The overall mode of action of Omomyc consists in the sequestration of Max and the concomitant competition of the Omomyc/Max complex with the endogenous c-Myc/Max heterodimer. This leads to the inhibition of the transactivation of Myc target genes involved in proliferation and metabolism. While this body of work has provided extraordinary insights to guide the future development of new cancer therapies that target c-Myc, Omomyc itself is not a therapeutic agent. In this context, we sought to exploit the use of a b-HLH-LZ to inhibit c-Myc in a cancer cell line in a more direct fashion. We demonstrate that the b-HLH-LZ domain of Max (Max* behaves as a bona fide protein transduction domain (PTD that can efficiently transduce across cellular membrane via through endocytosis and translocate to the nucleus. In addition, we show that the treatment of HeLa cells with Max* leads to a reduction of metabolism and proliferation rate. Accordingly, we observe a decrease of the population of HeLa cells in S phase, an accumulation in G1/G0 and the induction of apoptosis. In agreement with these phenotypic changes, we show by q-RT-PCR that the treatment of HeLa cells with Max* leads to the activation of the transcription c-Myc repressed genes as well as the repression of the expression of c-Myc activated genes. In addition to the novel discovery that the Max b-HLH-LZ is a PTD, our findings open up new avenues and strategies for the direct inhibition of c-Myc with b-HLH-LZ analogs.

  10. USP10 Antagonizes c-Myc Transcriptional Activation through SIRT6 Stabilization to Suppress Tumor Formation

    Directory of Open Access Journals (Sweden)

    Zhenghong Lin

    2013-12-01

    Full Text Available The reduced protein expression of SIRT6 tumor suppressor is involved in tumorigenesis. The molecular mechanisms underlying SIRT6 protein downregulation in human cancers remain unknown. Using a proteomic approach, we have identified the ubiquitin-specific peptidase USP10, another tumor suppressor, as one of the SIRT6-interacting proteins. USP10 suppresses SIRT6 ubiquitination to protect SIRT6 from proteasomal degradation. USP10 antagonizes the transcriptional activity of the c-Myc oncogene through SIRT6, as well as p53, to inhibit cell-cycle progression, cancer cell growth, and tumor formation. To support this conclusion, we detected significant reductions in both USP10 and SIRT6 protein expression in human colon cancers. Our study discovered crosstalk between two tumor-suppressive genes in regulating cell-cycle progression and proliferation and showed that dysregulated USP10 function promotes tumorigenesis through SIRT6 degradation.

  11. Transformation of follicular lymphoma to plasmablastic lymphoma with c-myc gene rearrangement.

    Science.gov (United States)

    Ouansafi, Ihsane; He, Bing; Fraser, Cory; Nie, Kui; Mathew, Susan; Bhanji, Rumina; Hoda, Rana; Arabadjief, Melissa; Knowles, Daniel; Cerutti, Andrea; Orazi, Attilio; Tam, Wayne

    2010-12-01

    Follicular lymphoma (FL) is an indolent lymphoma that transforms to high-grade lymphoma, mostly diffuse large B-cell lymphoma, in about a third of patients. We present the first report of a case of FL that transformed to plasmablastic lymphoma (PBL). Clonal transformation of the FL to PBL was evidenced by identical IGH/BCL2 gene rearrangements and VDJ gene usage in rearranged IGH genes. IGH/ BCL2 translocation was retained in the PBL, which also acquired c-myc gene rearrangement. Genealogic analysis based on somatic hypermutation of the rearranged IGH genes of both FL and PBL suggests that transformation of the FL to PBL occurred most likely by divergent evolution from a common progenitor cell rather than direct evolution from the FL clone. Our study of this unusual case expands the histologic spectrum of FL transformation and increases our understanding of the pathogenetic mechanisms of transformation of indolent lymphomas to aggressive lymphomas.

  12. Suppression of c-Myc induces apoptosis via an AMPK/mTOR-dependent pathway by 4-O-methyl-ascochlorin in leukemia cells.

    Science.gov (United States)

    Shin, Jae-Moon; Jeong, Yun-Jeong; Cho, Hyun-Ji; Magae, Junji; Bae, Young-Seuk; Chang, Young-Chae

    2016-05-01

    4-O-Methyl-ascochlorin (MAC) is a methylated derivative of the prenyl-phenol antibiotic ascochlorin, which was isolated from an incomplete fungus, Ascochyta viciae. Although the effects of MAC on apoptosis have been reported, the underlying mechanisms remain unknown. Here, we show that MAC promoted apoptotic cell death and downregulated c-Myc expression in K562 human leukemia cells. The effect of MAC on apoptosis was similar to that of 10058-F4 (a c-Myc inhibitor) or c-Myc siRNA, suggesting that the downregulation of c-Myc expression plays a role in the apoptotic effect of MAC. Further investigation showed that MAC downregulated c-Myc by inhibiting protein synthesis. MAC promoted the phosphorylation of AMP-activated protein kinase (AMPK) and inhibited the phosphorylation of mammalian target of rapamycin (mTOR) and its target proteins, including p70S6 K and 4E-BP-1. Treatment of cells with AICAR (an AMPK activator), rapamycin (an mTOR inhibitor), or mTOR siRNA downregulated c-Myc expression and induced apoptosis to a similar extent to that of MAC. These results suggest that the effect of MAC on apoptosis induction in human leukemia cells is mediated by the suppression of c-Myc protein synthesis via an AMPK/mTOR-dependent mechanism.

  13. The long non-coding RNA GAS5 cooperates with the eukaryotic translation initiation factor 4E to regulate c-Myc translation.

    Directory of Open Access Journals (Sweden)

    Guangzhen Hu

    Full Text Available Long noncoding RNAs (lncRNAs are important regulators of transcription; however, their involvement in protein translation is not well known. Here we explored whether the lncRNA GAS5 is associated with translation initiation machinery and regulates translation. GAS5 was enriched with eukaryotic translation initiation factor-4E (eIF4E in an RNA-immunoprecipitation assay using lymphoma cell lines. We identified two RNA binding motifs within eIF4E protein and the deletion of each motif inhibited the binding of GAS5 with eIF4E. To confirm the role of GAS5 in translation regulation, GAS5 siRNA and in vitro transcribed GAS5 RNA were used to knock down or overexpress GAS5, respectively. GAS5 siRNA had no effect on global protein translation but did specifically increase c-Myc protein level without an effect on c-Myc mRNA. The mechanism of this increase in c-Myc protein was enhanced association of c-Myc mRNA with the polysome without any effect on protein stability. In contrast, overexpression of in vitro transcribed GAS5 RNA suppressed c-Myc protein without affecting c-Myc mRNA. Interestingly, GAS5 was found to be bound with c-Myc mRNA, suggesting that GAS5 regulates c-Myc translation through lncRNA-mRNA interaction. Our findings have uncovered a role of GAS5 lncRNA in translation regulation through its interactions with eIF4E and c-Myc mRNA.

  14. Fish Catch Composition of Artisanal and Bottom Trawl Fisheries in ...

    African Journals Online (AJOL)

    to grow and active management is crucial to reduce resource user conflict. Corresponding .... reefs, islets, sandy shores and tidal flats are ..... conflict. The direct benefits of BRDs to users ... Gobert B (1994) Size structures of demersal catches ...

  15. Catch bonding in the forced dissociation of a polymer endpoint

    Science.gov (United States)

    Vrusch, Cyril; Storm, Cornelis

    2018-04-01

    Applying a force to certain supramolecular bonds may initially stabilize them, manifested by a lower dissociation rate. We show that this behavior, known as catch bonding and by now broadly reported in numerous biophysics bonds, is generically expected when either or both the trapping potential and the force applied to the bond possess some degree of nonlinearity. We enumerate possible scenarios and for each identify the possibility and, if applicable, the criterion for catch bonding to occur. The effect is robustly predicted by Kramers theory and Mean First Passage Time theory and confirmed in direct molecular dynamics simulation. Among the catch scenarios, one plays out essentially any time the force on the bond originates in a polymeric object, implying that some degree of catch bond behavior is to be expected in many settings relevant to polymer network mechanics or optical tweezer experiments.

  16. Thailand's Missing Marine Fisheries Catch (1950–2014

    Directory of Open Access Journals (Sweden)

    Brittany Derrick

    2017-12-01

    Full Text Available Overexploitation of marine resources has led to declining catches in many countries worldwide, and often also leads to fishing effort being exported to waters of neighboring countries or high seas areas. Thailand is currently under pressure to curb illegal fishing and human rights violations within its distant water fleets or face a European Union import ban. Simultaneously, Thailand is attempting to reduce fishing effort within its Exclusive Economic Zone (EEZ. Crucial to these endeavors is a comprehensive knowledge of total fisheries catches over time. A reconstruction of fisheries catches within Thailand's EEZ and by Thailand's fleet in neighboring countries' EEZs was undertaken for 1950–2014 to derive a comprehensive historical time series of total catches. This includes landings and discards that were not accounted for in official, reported statistics. Reconstructed Thai catches from within Thailand's EEZ increased from approximately 400,000 t·year−1 in 1950 to a peak of 2.6 million t·year−1 in 1987, before declining to around 1.7 million t·year−1 in 2014. Catches taken by Thai vessels outside their own EEZ increased from 52,000 t·year−1 in 1965 to a peak of 7.6 million t·year−1 in 1996, before declining to around 3.7 million t·year−1 by 2014. In total, reconstructed catches were estimated to be nearly three times larger than data reported by Thailand to the Food and Agriculture Organization of the United Nations (FAO. Reconstructed Thai distant-water fleet catches were almost seven times higher than the comparable non-domestic catch deemed reported for Thailand. Thai landings from recreational fishing were conservatively estimated for the first time, and while they contributed less than 1% of current catch, they can be expected to grow in volume and importance with increasing tourism. As Thailand takes measures to reduce fishing effort within its EEZs and increases monitoring and enforcement of illegal and foreign

  17. Test Plan for Measuring Ventilation Rates and Combustible Gas Levels in TWRS Active Catch Tanks

    Energy Technology Data Exchange (ETDEWEB)

    NGUYEN, D.M.

    1999-10-25

    The purpose of this sampling activity is to obtain data to support an initial evaluation of potential hazards due to the presence of combustible gas in catch tanks that are currently operated by the River Protection Project (RPP). Results of the hazard analysis will be used to support closure of the flammable gas unreviewed safety question for these facilities. The data collection will be conducted in accordance with the Tank Safety Screening Data Quality Objective (Dukelow et al. 1995). Combustible gas, ammonia, and organic vapor levels in the headspace of the catch tanks will be field-measured using hand-held instruments. If a combustible gas level measurement in a tank exceeds an established threshold, gas samples will he collected in SUMMA' canisters for more extensive laboratory analysis. In addition, ventilation rates of some catch tanks will be measured to evaluate removal of flammable gas by air flowing through the tanks. This test plan identifies the sample collection, laboratory analysis, quality assurance, and reporting objectives for this data collection effort. The plan also provides the procedures for field measurement of combustible gas concentrations and ventilation rates.

  18. Catch, effort and bycatch of longline fishery in central Peru

    Directory of Open Access Journals (Sweden)

    Liliana Ayala

    2014-12-01

    Full Text Available The aims of this study is to report some characteristics of fishing trips, effort, catches, fishing areas and bycatch through observations on board and logbooks. 85% of sets were in the first 574 Km of distance from the coast (309 nautical miles. Farthest set was located at 1320 Km (712 nautical miles. A total of 382000 hooks were used to catch Mahi mahi, in 224 sets and 29 fishing trips, 94.6% of catch was Mahi mahi, 2.7% blue shark (Prionace glauca y 1,3% mako shark (Isurus oxyrinchus. Also, 103790 hooks were used to catch sharks, in 109 sets y 12 trips, 81.9% of catch was blue sharks and 16.8% mako sharks. Catch per Unit of Effort (CPUE for Mahi mahi shows significative difference among seasons; with a peak from November to January. CPUE for shark shows significative difference among seasons, with peaks in September and October. The Green turtle Chelonia mydas agassizii was the most caugth species and two of three were juveniles. All Loggerhead turtles, Caretta caretta, caught were juveniles. A petrel is reported as bycatch and, probably, mammal bycatch is scarce. Considering the huge effort of this fishery, it is important to monitor it and establish management actions.

  19. Use of a transfected and amplified Drosophila heat shock promoter construction for inducible production of toxic mouse c-myc proteins in CHO cells

    International Nuclear Information System (INIS)

    Wurm, F.M.; Gwinn, K.A.; Papoulas, O.; Pallavicini, M.; Kingston, R.E.

    1987-01-01

    After transfection and selection with methotrexate, CHO cell lines were established which contained up to 2000 copies of an expression vector for c-myc protein. The vector contained the Drosophila heat shock protein 70 (hsp70) promoter fused with the coding region of the mouse c-myc gene. Incubation of cells for up to 3 hours at 43 0 C resulted in at least a 100-fold induction of recombinant c-myc mRNA. When cells were shifted back to 37 0 C, within 1 to 4 hours, this RNA was translated into protein to yield about 250 μg per 10 9 cells. Cells died a few hours later, suggesting that high concentrations of intracellular c-myc are cytotoxic. 47 refs., 5 figs

  20. Immortalization of human neural stem cells with the c-myc mutant T58A.

    Directory of Open Access Journals (Sweden)

    Lidia De Filippis

    Full Text Available Human neural stem cells (hNSC represent an essential source of renewable brain cells for both experimental studies and cell replacement therapies. Their relatively slow rate of proliferation and physiological senescence in culture make their use cumbersome under some experimental and pre-clinical settings. The immortalization of hNSC with the v-myc gene (v-IhNSC has been shown to generate stem cells endowed with enhanced proliferative capacity, which greatly facilitates the study of hNSCs, both in vitro and in vivo. Despite the excellent safety properties displayed by v-IhNSCs--which do not transform in vitro and are not tumorigenic in vivo--the v-myc gene contains several mutations and recombination elements, whose role(s and effects remains to be elucidated, yielding unresolved safety concerns. To address this issue, we used a c-myc T58A retroviral vector to establish an immortal cell line (T-IhNSC from the same hNSCs used to generate the original v-IhNSCs and compared their characteristics with the latter, with hNSC and with hNSC immortalized using c-myc wt (c-IhNSC. T-IhNSCs displayed an enhanced self-renewal ability, with their proliferative capacity and clonogenic potential being remarkably comparable to those of v-IhNSC and higher than wild type hNSCs and c-IhNSCs. Upon growth factors removal, T-IhNSC promptly gave rise to well-differentiated neurons, astrocytes and most importantly, to a heretofore undocumented high percentage of human oligodendrocytes (up to 23%. Persistent growth-factor dependence, steady functional properties, lack of ability to generate colonies in soft-agar colony-forming assay and to establish tumors upon orthotopic transplantation, point to the fact that immortalization by c-myc T58A does not bring about tumorigenicity in hNSCs. Hence, this work describes a novel and continuous cell line of immortalized human multipotent neural stem cells, in which the immortalizing agent is represented by a single gene which, in

  1. Melatonin disturbs SUMOylation mediated crosstalk between c-Myc and Nestin via MT1 activation and promotes the sensitivity of Paclitaxel in brain cancer stem cells.

    Science.gov (United States)

    Lee, Hyemin; Lee, Hyo-Jung; Jung, Ji Hoon; Shin, Eun Ah; Kim, Sung-Hoon

    2018-04-14

    Here the underlying antitumor mechanism of melatonin and its potency as a sensitizer of Paclitaxel was investigated in X02 cancer stem cells. Melatonin suppressed sphere formation and induced G2/M arrest in X02 cells expressing Nestin, CD133, CXCR4 and SOX-2 as biomarkers of stemness. Furthermore, melatonin reduced the expression of CDK2, CDK4, cyclin D1, cyclin E, and c-Myc and upregulated cyclin B1 in X02 cells. Notably, genes of c-Myc related mRNAs were differentially expressed in melatonin treated X02 cells by microarray analysis. Consistently, melatonin reduced the expression of c-Myc at mRNA and protein levels, which was blocked by MG132. Of note, overexpression of c-Myc increased the expression of Nestin, while overexpression of Nestin enhanced c-Myc through crosstalk despite different locations, nucleus and cytoplasm. Interestingly, melatonin attenuated small ubiquitin-related modifier-1 (SUMO-1) more than SUMO-2 or SUMO-3 and disturbed nuclear translocation of Nestin for direct binding to c-Myc by SUMOylation of SUMO-1 protein by immunofluorescence and immunoprecipitation. Also, melatonin reduced trimethylated histone H3K4me3 and H3K36me3 more than dimethylation in X02 cells by Western blotting and Chromatin immunoprecipitation assay. Notably, melatonin upregulated MT1, not MT2, in X02 cells and melatonin receptor inhibitor Luzindole blocked the ability of melatonin to decrease the expression of Nestin, p-c-Myc(S62) and c-Myc. Furthermore, melatonin promoted cytotoxicity, sub G1 accumulation and apoptotic body formation by Paclitaxcel in X02 cells. Taken together, these findings suggest that melatonin inhibits stemness via suppression of c-Myc, Nestin, and histone methylation via MT1 activation and promotes anticancer effect of Paclitaxcel in brain cancer stem cells. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.

  2. Integrin α6Bβ4 inhibits colon cancer cell proliferation and c-Myc activity

    International Nuclear Information System (INIS)

    Dydensborg, Anders Bondo; Teller, Inga C; Groulx, Jean-François; Basora, Nuria; Paré, Fréderic; Herring, Elizabeth; Gauthier, Rémy; Jean, Dominique; Beaulieu, Jean-François

    2009-01-01

    Integrins are known to be important contributors to cancer progression. We have previously shown that the integrin β4 subunit is up-regulated in primary colon cancer. Its partner, the integrin α6 subunit, exists as two different mRNA splice variants, α6A and α6B, that differ in their cytoplasmic domains but evidence for distinct biological functions of these α6 splice variants is still lacking. In this work, we first analyzed the expression of integrin α6A and α6B at the protein and transcript levels in normal human colonic cells as well as colorectal adenocarcinoma cells from both primary tumors and established cell lines. Then, using forced expression experiments, we investigated the effect of α6A and α6B on the regulation of cell proliferation in a colon cancer cell line. Using variant-specific antibodies, we observed that α6A and α6B are differentially expressed both within the normal adult colonic epithelium and between normal and diseased colonic tissues. Proliferative cells located in the lower half of the glands were found to predominantly express α6A, while the differentiated and quiescent colonocytes in the upper half of the glands and surface epithelium expressed α6B. A relative decrease of α6B expression was also identified in primary colon tumors and adenocarcinoma cell lines suggesting that the α6A/α6B ratios may be linked to the proliferative status of colonic cells. Additional studies in colon cancer cells showed that experimentally restoring the α6A/α6B balance in favor of α6B caused a decrease in cellular S-phase entry and repressed the activity of c-Myc. The findings that the α6Bβ4 integrin is expressed in quiescent normal colonic cells and is significantly down-regulated in colon cancer cells relative to its α6Aβ4 counterpart are consistent with the anti-proliferative influence and inhibitory effect on c-Myc activity identified for this α6Bβ4 integrin. Taken together, these findings point out the importance of integrin

  3. Intensive Monitoring Survey of Nearby Galaxies (IMSNG): Catching Early Light Curves of Supernovae

    Science.gov (United States)

    Im, Myungshin; IMSNG Team

    2018-01-01

    SNe light curves have been used to study the expansion history of the universe, and a lot of efforts have gone into understanding the overall shape of the radioactively powered light curve. However, we still have little direct observational evidence for the theorized SN progenitor systems. Recent studies suggest that the light curve of a supernova shortly after its explosion (world. Through this survey, we expect to catch the very early precursor emission as faint as R=21 mag (~0.1 Rsun for the progenitor). This poster outlines this project, and present a few scientific highlights, such as the early light curve of SN 2015F in NGC 2442.

  4. Endoglin inhibits ERK-induced c-Myc and cyclin D1 expression to impede endothelial cell proliferation

    Energy Technology Data Exchange (ETDEWEB)

    Pan, Christopher C.; Bloodworth, Jeffrey C. [Division of Pharmacology, Columbus, OH 43210 (United States); Mythreye, Karthikeyan [Duke University, Department of Medicine, Durham, NC 27708 (United States); Lee, Nam Y., E-mail: lee.5064@osu.edu [Division of Pharmacology, Columbus, OH 43210 (United States); Davis Heart and Lung Research Institute, Columbus, OH 43210 (United States)

    2012-08-03

    Highlights: Black-Right-Pointing-Pointer Endoglin inhibits ERK activation in endothelial cells. Black-Right-Pointing-Pointer Endoglin is a regulator of c-Myc and cyclin D1 expression. Black-Right-Pointing-Pointer {beta}-arrestin2 interaction with endoglin is required for ERK/c-Myc repression. Black-Right-Pointing-Pointer Endoglin impedes cellular proliferation by targeting ERK-induced mitogenic signaling. -- Abstract: Endoglin is an endothelial-specific transforming growth factor beta (TGF-{beta}) co-receptor essential for angiogenesis and vascular remodeling. Endoglin regulates a wide range of cellular processes, including cell adhesion, migration, and proliferation, through TGF-{beta} signaling to canonical Smad and Smad-independent pathways. Despite its overall pro-angiogenic role in the vasculature, the underlying mechanism of endoglin action is poorly characterized. We previously identified {beta}-arrestin2 as a binding partner that causes endoglin internalization from the plasma membrane and inhibits ERK signaling towards endothelial migration. In the present study, we examined the mechanistic role of endoglin and {beta}-arrestin2 in endothelial cell proliferation. We show that endoglin impedes cell growth through sustained inhibition of ERK-induced c-Myc and cyclin D1 expression in a TGF-{beta}-independent manner. The down-regulation of c-Myc and cyclin D1, along with growth-inhibition, are reversed when the endoglin/{beta}-arrestin2 interaction is disrupted. Given that TGF-{beta}-induced Smad signaling potently represses c-Myc in most cell types, our findings here show a novel mechanism by which endoglin augments growth-inhibition by targeting ERK and key downstream mitogenic substrates.

  5. Conserved features of cancer cells define their sensitivity to HAMLET-induced death; c-Myc and glycolysis.

    Science.gov (United States)

    Storm, P; Aits, S; Puthia, M K; Urbano, A; Northen, T; Powers, S; Bowen, B; Chao, Y; Reindl, W; Lee, D Y; Sullivan, N L; Zhang, J; Trulsson, M; Yang, H; Watson, J D; Svanborg, C

    2011-12-01

    HAMLET is the first member of a new family of tumoricidal protein-lipid complexes that kill cancer cells broadly, while sparing healthy, differentiated cells. Many and diverse tumor cell types are sensitive to the lethal effect, suggesting that HAMLET identifies and activates conserved death pathways in cancer cells. Here, we investigated the molecular basis for the difference in sensitivity between cancer cells and healthy cells. Using a combination of small-hairpin RNA (shRNA) inhibition, proteomic and metabolomic technology, we identified the c-Myc oncogene as one essential determinant of HAMLET sensitivity. Increased c-Myc expression levels promoted sensitivity to HAMLET and shRNA knockdown of c-Myc suppressed the lethal response, suggesting that oncogenic transformation with c-Myc creates a HAMLET-sensitive phenotype. Furthermore, HAMLET sensitivity was modified by the glycolytic state of tumor cells. Glucose deprivation sensitized tumor cells to HAMLET-induced cell death and in the shRNA screen, hexokinase 1 (HK1), 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 and hypoxia-inducible factor 1α modified HAMLET sensitivity. HK1 was shown to bind HAMLET in a protein array containing ∼8000 targets, and HK activity decreased within 15 min of HAMLET treatment, before morphological signs of tumor cell death. In parallel, HAMLET triggered rapid metabolic paralysis in carcinoma cells. Tumor cells were also shown to contain large amounts of oleic acid and its derivatives already after 15 min. The results identify HAMLET as a novel anti-cancer agent that kills tumor cells by exploiting unifying features of cancer cells such as oncogene addiction or the Warburg effect.

  6. Conserved features of cancer cells define their sensitivity of HAMLET-induced death; c-Myc and glycolysis

    Science.gov (United States)

    Storm, Petter; Puthia, Manoj Kumar; Aits, Sonja; Urbano, Alexander; Northen, Trent; Powers, Scott; Bowen, Ben; Chao, Yinxia; Reindl, Wolfgang; Lee, Do Yup; Sullivan, Nancy Liu; Zhang, Jianping; Trulsson, Maria; Yang, Henry; Watson, James; Svanborg, Catharina

    2014-01-01

    HAMLET is the first member of a new family of tumoricidal protein-lipid complexes that kill cancer cells broadly, while sparing healthy, differentiated cells. Many and diverse tumor cell types are sensitive to the lethal effect, suggesting that HAMLET identifies and activates conserved death pathways in cancer cells. Here we investigated the molecular basis for the difference in sensitivity between cancer cells and healthy cells. Using a combination of small hairpin RNA inhibition, proteomic and metabolomic technology we identified the c-Myc oncogene as one essential determinant of HAMLET sensitivity. Increased c-Myc expression levels promoted the sensitivity to HAMLET and shRNA knockdown of c-Myc suppressed the lethal response, suggesting that oncogenic transformation with c-Myc creates a HAMLET-sensitive phenotype. Furthermore, the HAMLET sensitivity was modified by the glycolytic state of the tumor cells. Glucose deprivation sensitized tumor cells to HAMLET-induced cell death and in the shRNA screen Hexokinase 1, PFKFB1 and HIF1α modified HAMLET sensitivity. Hexokinase 1 was shown to bind HAMLET in a protein array containing approximately 8000 targets and Hexokinase activity decreased within 15 minutes of HAMLET treatment, prior to morphological signs of tumor cell death. In parallel, HAMLET triggered rapid metabolic paralysis in carcinoma cells. The glycolytic machinery was modified and glycolysis was shifted towards the pentose phosphate pathway. Tumor cells were also shown to contain large amounts of oleic acid and its derivatives already after 15 minutes. The results identify HAMLET as a novel anti-cancer agent that kills tumor cells by exploiting unifying features of cancer cells such as oncogene-addiction or the Warburg effect. PMID:21643007

  7. Pre-clinical analysis of changes in intra-cellular biochemistry of glioblastoma multiforme (GBM) cells due to c-Myc silencing.

    Science.gov (United States)

    Rajagopalan, Vishal; Vaidyanathan, Muthukumar; Janardhanam, Vanisree Arambakkam; Bradner, James E

    2014-10-01

    Glioblastoma Multiforme (GBM) is an aggressive form of brain Tumor that has few cures. In this study, we analyze the anti-proliferative effects of a new molecule JQ1 against GBMs induced in Wistar Rats. JQ1 is essentially a Myc inhibitor. c-Myc is also known for altering the biochemistry of a tumor cell. Therefore, the study is intended to analyze certain other oncogenes associated with c-Myc and also the change in cellular biochemistry upon c-Myc inhibition. The quantitative analysis of gene expression gave a co-expressive pattern for all the three genes involved namely; c-Myc, Bcl-2, and Akt. The cellular biochemistry analysis by transmission electron microscopy revealed high glycogen and lipid aggregation in Myc inhibited cells and excessive autophagy. The study demonstrates the role of c-Myc as a central metabolic regulator and Bcl-2 and Akt assisting in extending c-Myc half-life as well as in regulation of autophagy, so as to regulate cell survival on the whole. The study also demonstrates that transient treatment by JQ1 leads to aggressive development of tumor and therefore, accelerating death, emphasizing the importance of dosage fixation, and duration for clinical use in future.

  8. Combined use of nuclear phosphoprotein c-Myc and cellular phosphoprotein p53 for hepatocellular carcinoma detection in high-risk chronic hepatitis C patients.

    Science.gov (United States)

    Attallah, A M; El-Far, M; Abdelrazek, M A; Omran, M M; Attallah, A A; Elkhouly, A A; Elkenawy, H M; Farid, K

    2017-10-01

    Hepatocellular carcinoma (HCC) is a multistage process resulting from various genetic changes. We aimed to determine nuclear phosphoprotein c-Myc and cellular phosphoprotein p53 expression and to evaluate their importance in HCC diagnosis. One hundred and twenty chronic hepatitis C (CHC) patients (60 non-HCC CHC patients and 60 HCC patients who had a single small (c-Myc and p53 were identified in liver tissues and serum samples using immunostaining, western blot and ELISA. Immunohistochemical detection of c-Myc and p53 with monospecific antibodies revealed intense and diffuse cytoplasmic staining patterns. Accumulated mutant proteins, released from tumour cells into the extracellular serum, were detected at 62 KDa, for c-Myc, and 53 KDa, for p53, using western blotting. In contrast to alpha feto-protein, there was a significant increase (p c-Myc (86.7% vs. 6.7%) and p53 (78.3% vs. 8.3%) in the malignant vs. non-malignant patients. The parallel combination of c-Myc and p53 reach the absolute sensitivity (100%), for more accurate and reliable HCC detection (specificity was 87%). c-Myc and p53 are potential HCC diagnostic biomarkers, and convenient combinations of them could improve diagnostic accuracy of HCC.

  9. Signature of ocean warming in global fisheries catch.

    Science.gov (United States)

    Cheung, William W L; Watson, Reg; Pauly, Daniel

    2013-05-16

    Marine fishes and invertebrates respond to ocean warming through distribution shifts, generally to higher latitudes and deeper waters. Consequently, fisheries should be affected by 'tropicalization' of catch (increasing dominance of warm-water species). However, a signature of such climate-change effects on global fisheries catch has so far not been detected. Here we report such an index, the mean temperature of the catch (MTC), that is calculated from the average inferred temperature preference of exploited species weighted by their annual catch. Our results show that, after accounting for the effects of fishing and large-scale oceanographic variability, global MTC increased at a rate of 0.19 degrees Celsius per decade between 1970 and 2006, and non-tropical MTC increased at a rate of 0.23 degrees Celsius per decade. In tropical areas, MTC increased initially because of the reduction in the proportion of subtropical species catches, but subsequently stabilized as scope for further tropicalization of communities became limited. Changes in MTC in 52 large marine ecosystems, covering the majority of the world's coastal and shelf areas, are significantly and positively related to regional changes in sea surface temperature. This study shows that ocean warming has already affected global fisheries in the past four decades, highlighting the immediate need to develop adaptation plans to minimize the effect of such warming on the economy and food security of coastal communities, particularly in tropical regions.

  10. MYCT1-TV, A Novel MYCT1 Transcript, Is Regulated by c-Myc and May Participate in Laryngeal Carcinogenesis

    Science.gov (United States)

    Fu, Shuang; Guo, Yan; Chen, Hong; Xu, Zhen-Ming; Qiu, Guang-Bin; Zhong, Ming; Sun, Kai-Lai; Fu, Wei-Neng

    2011-01-01

    Background MYCT1, a putative target of c-Myc, is a novel candidate tumor suppressor gene cloned from laryngeal squamous cell carcinoma (LSCC). Its transcriptional regulation and biological effects on LSCC have not been clarified. Methodology/Principal Findings Using RACE assay, we cloned a 1106 bp transcript named Myc target 1 transcript variant 1 (MYCT1-TV) and confirmed its transcriptional start site was located at 140 bp upstream of the ATG start codon of MYCT1-TV. Luciferase, electrophoretic mobility shift and chromatin immunoprecipitation assays confirmed c-Myc could regulate the promoter activity of MYCT1-TV by specifically binding to the E-box elements within −886 to −655 bp region. These results were further verified by site-directed mutagenesis and RNA interference (RNAi) assays. MYCT1-TV and MYCT1 expressed lower in LSCC than those in paired adjacent normal laryngeal tissues, and overexpression of MYCT1-TV and MYCT1 could inhibit cell proliferation and invasion and promote apoptosis in LSCC cells. Conclusions/Significance Our data indicate that MYCT1-TV, a novel MYCT1 transcript, is regulated by c-Myc and down-regulation of MYCT1-TV/MYCT1 could contribute to LSCC development and function. PMID:21998677

  11. Effect of c-myc on the ultrastructural structure of cochleae in guinea pigs with noise induced hearing loss

    International Nuclear Information System (INIS)

    Han, Yu; Zhong, Cuiping; Hong, Liu; Wang, Ye; Qiao, Li; Qiu, Jianhua

    2009-01-01

    Noise over-stimulation may induce hair cells loss and hearing deficit. The c-myc oncogene is a major regulator for cell proliferation, growth, and apoptosis. However, the role of this gene in the mammalian cochlea is still unclear. The study was designed to firstly investigate its function under noise condition, from the aspect of cochlear ultrastructural changes. We had established the adenoviral vector of c-myc gene and delivered the adenovirus suspension into the scala tympani of guinea pigs 4 days before noise exposure. The empty adenoviral vectors were injected as control. Then, all subjects were exposed to 4-kHz octave-band noise at 110 dB SPL for 8 h/day, 3 days consecutively. Auditory thresholds were assessed by auditory brainstem response, prior to and 7 days following noise exposure. On the seventh days after noise exposure, the cochlear sensory epithelia surface was observed microscopically and the cochleae were taken to study the ultrastructural changes. The results indicated that auditory threshold shift after noise exposure was higher in the ears treated with Ad.EGFP than that treated with Ad.c-myc-EGFP. Stereocilia loss and the disarrangement of outer hair cells were observed, with greater changes found in the Ad.EGFP group. Also, the ultrastructure changes were severe in the Ad.EGFP group, but not obvious in the Ad.c-myc-EGFP group. Therefore, c-myc gene might play an unexpected role in hearing functional and morphological protection from acoustic trauma.

  12. Effect of c-myc on the ultrastructural structure of cochleae in guinea pigs with noise induced hearing loss

    Energy Technology Data Exchange (ETDEWEB)

    Han, Yu; Zhong, Cuiping [Department of Otolaryngology, Xijing Hospital, Fourth Military Medical University, Xi' an, 710032 Shaanxi Province (China); Hong, Liu [First Division of Digestive Diseases, Xijing Hospital, Fourth Military Medical University, Xi' an, 710032 Shaanxi Province (China); Wang, Ye; Qiao, Li [Department of Otolaryngology, Xijing Hospital, Fourth Military Medical University, Xi' an, 710032 Shaanxi Province (China); Qiu, Jianhua, E-mail: qiujh@fmmu.edu.cn [Department of Otolaryngology, Xijing Hospital, Fourth Military Medical University, Xi' an, 710032 Shaanxi Province (China)

    2009-12-18

    Noise over-stimulation may induce hair cells loss and hearing deficit. The c-myc oncogene is a major regulator for cell proliferation, growth, and apoptosis. However, the role of this gene in the mammalian cochlea is still unclear. The study was designed to firstly investigate its function under noise condition, from the aspect of cochlear ultrastructural changes. We had established the adenoviral vector of c-myc gene and delivered the adenovirus suspension into the scala tympani of guinea pigs 4 days before noise exposure. The empty adenoviral vectors were injected as control. Then, all subjects were exposed to 4-kHz octave-band noise at 110 dB SPL for 8 h/day, 3 days consecutively. Auditory thresholds were assessed by auditory brainstem response, prior to and 7 days following noise exposure. On the seventh days after noise exposure, the cochlear sensory epithelia surface was observed microscopically and the cochleae were taken to study the ultrastructural changes. The results indicated that auditory threshold shift after noise exposure was higher in the ears treated with Ad.EGFP than that treated with Ad.c-myc-EGFP. Stereocilia loss and the disarrangement of outer hair cells were observed, with greater changes found in the Ad.EGFP group. Also, the ultrastructure changes were severe in the Ad.EGFP group, but not obvious in the Ad.c-myc-EGFP group. Therefore, c-myc gene might play an unexpected role in hearing functional and morphological protection from acoustic trauma.

  13. Apoptosis and cell proliferation in the development of gastric carcinomas: associations with c-myc and p53 protein expression.

    Science.gov (United States)

    Ishii, Hideaki H; Gobé, Glenda C; Pan, Wenshen; Yoneyama, Juichi; Ebihara, Yoshiro

    2002-09-01

    Patients with gastric carcinomas have a poor prognosis and low survival rates. The aim of the present paper was to characterize cellular and molecular properties to provide insight into aspects of tumor progression in early compared with advanced gastric cancers. One hundred and nine graded gastric carcinomas (early or advanced stage, undifferentiated or differentiated type) with paired non-cancer tissue were studied to define the correlation between apoptosis (morphology, terminal deoxyribonucleotidyl transferase-mediated dUTP-digoxigenin nick end-labeling), cell proliferation (Ki-67 expression, morphology) and expression and localization of two proteins frequently having altered expression in cancers, namely p53 and c-myc. Overall, apoptosis was lower in early stage, differentiated and undifferentiated gastric carcinomas compared with advanced-stage cancers. Cell proliferation was comparatively high in all stages. There was a high level of p53 positivity in all stages. Only the early- and advanced-stage undifferentiated cancers that were p53 positive had a significantly higher level of apoptosis (P cancers that had either c-myc or p53-positivity. The results indicate that low apoptosis and high cell proliferation combine to drive gastric cancer development. The molecular controls for high cell proliferation of the early stage undifferentiated gastric cancers involve overexpression of both p53 and c-myc. Overexpression of p53 may also control cancer development in that its expression is associated with higher levels of apoptosis in early and late-stage undifferentiated, cancers. Copyright 2002 Blackwell Publishing Asia Pty Ltd

  14. The small Rho GTPase Rac1 controls normal human dermal fibroblasts proliferation with phosphorylation of the oncoprotein c-myc

    International Nuclear Information System (INIS)

    Nikolova, Ekaterina; Mitev, Vanio; Zhelev, Nikolai; Deroanne, Christophe F.; Poumay, Yves

    2007-01-01

    Proliferation of dermal fibroblasts is crucial for the maintenance of skin. The small Rho GTPase, Rac1, has been identified as a key transducer of proliferative signals in various cell types, but in normal human dermal fibroblasts its significance to cell growth control has not been studied. In this study, we applied the method of RNA interference to suppress endogenous Rac1 expression and examined the consequences on human skin fibroblasts. Rac1 knock-down resulted in inhibition of DNA synthesis. This effect was not mediated by inhibition of the central transducer of proliferative stimuli, ERK1/2 or by activation of the pro-apoptotic p38. Rather, as a consequence of the suppressed Rac1 expression we observed a significant decrease in phosphorylation of c-myc, revealing for the first time that in human fibroblasts Rac1 exerts control on proliferation through c-myc phosphorylation. Thus Rac1 activates proliferation of normal fibroblasts through stimulation of c-myc phosphorylation without affecting ERK1/2 activity

  15. Encapsulation of c-myc antisense oligodeoxynucleotides in lipid particles improves antitumoral efficacy in vivo in a human melanoma line.

    Science.gov (United States)

    Leonetti, C; Biroccio, A; Benassi, B; Stringaro, A; Stoppacciaro, A; Semple, S C; Zupi, G

    2001-06-01

    Phosphorothioate c-myc antisense oligodeoxynucleotides [S]ODNs (free INX-6295) were encapsulated in a new liposome formulation and the antitumor activity was compared to the unencapsulated antisense in a human melanoma xenograft. The systemic administration of INX-6295 encapsulated in stabilized antisense lipid particles (SALP INX-6295) improved plasma AUC (area under the plasma concentration-time curve) and initial half-life of free INX-6295, resulting in a significant enhancement in tumor accumulation and improvement in tumor distribution of antisense oligodeoxynucleotides. Animals treated with SALP INX-6295 exhibited a prolonged reduction of c-myc expression, reduced tumor growth and increased mice survival. When administered in combination with cisplatin (DDP), SALP INX-6295 produced a complete tumor regression in approximately 30% of treated mice, which persisted for at least 60 days following the first cycle of treatment. Finally, the median survival of mice treated with DDP/SALP INX-6295 increased by 105% compared to 84% for animals treated with the combination DDP/free INX-6295. These data indicate that the biological activity and the therapeutic efficacy of c-myc antisense therapy may be improved when these agents are administered in lipid-based delivery systems.

  16. Immunodetection of rasP21 and c-myc oncogenes in oral mucosal swab preparation from clove cigarette smokers

    Directory of Open Access Journals (Sweden)

    Silvi Kintawati

    2008-12-01

    Full Text Available Background: Smoking is the biggest factor for oral cavity malignancy. Some carcinogens found in cigar will stimulate epithel cell in oral cavity and cause mechanism disturbance on tissue resistance and produce abnormal genes (oncogenes. Oncogenes ras and myc are found on malignant tumor in oral cavity which are associated with smoking. Purpose: This research is to find the expression of oncogenes rasP21 and c-myc in oral mucosa epithelial of smoker with immunocytochemistry reaction. Methods: An oral mucosal swab was performed to 30 smokers categorized as light, moderate, and chain, and 10 non smokers which was followed by immunocytochemistry reaction using antibody towards oncogene rasP21 and c-myc is reacted to identify the influence of smoking towards malignant tumor in oral cavity. The result is statistically analyzed using Kruskal-Wallis test. Result: Based on the observation result of oncogene rasP21reaction, it shows that there is significant difference between non smoker group and light smoker, compared to moderate and chain smoker group (p < 0.01. On the other side, the observation result of oncogene c-myc indicates that there is no significant difference between the group of non smokers and the group of light, moderate, and chain smokers (p > 0.05. Conclusion: The higher the possibility of oral cavity malignancy and that the antibody for rasP21 oncogene can be used as a marker for early detection of oral cavity malignancy caused by smoking.

  17. Nac1 promotes self-renewal of embryonic stem cells through direct transcriptional regulation of c-Myc.

    Science.gov (United States)

    Ruan, Yan; He, Jianrong; Wu, Wei; He, Ping; Tian, Yanping; Xiao, Lan; Liu, Gaoke; Wang, Jiali; Cheng, Yuda; Zhang, Shuo; Yang, Yi; Xiong, Jiaxiang; Zhao, Ke; Wan, Ying; Huang, He; Zhang, Junlei; Jian, Rui

    2017-07-18

    The pluripotency transcriptional network in embryonic stem cells (ESCs) is composed of distinct functional units including the core and Myc units. It is hoped that dissection of the cellular functions and interconnections of network factors will aid our understanding of ESC and cancer biology. Proteomic and genomic approaches have identified Nac1 as a member of the core pluripotency network. However, previous studies have predominantly focused on the role of Nac1 in psychomotor stimulant response and cancer pathogenesis. In this study, we report that Nac1 is a self-renewal promoting factor, but is not required for maintaining pluripotency of ESCs. Loss of function of Nac1 in ESCs results in a reduced proliferation rate and an enhanced differentiation propensity. Nac1 overexpression promotes ESC proliferation and delays ESC differentiation in the absence of leukemia inhibitory factor (LIF). Furthermore, we demonstrated that Nac1 directly binds to the c-Myc promoter and regulates c-Myc transcription. The study also revealed that the function of Nac1 in promoting ESC self-renewal appears to be partially mediated by c-Myc. These findings establish a functional link between the core and c-Myc-centered networks and provide new insights into mechanisms of stemness regulation in ESCs and cancer.

  18. Effects on micronuclei formation of 60-Hz electromagnetic field exposure with ionizing radiation, hydrogen peroxide, or c-Myc overexpression.

    Science.gov (United States)

    Jin, Yeung Bae; Kang, Ga-Young; Lee, Jae Seon; Choi, Jong-Il; Lee, Ju-Woon; Hong, Seung-Cheol; Myung, Sung Ho; Lee, Yun-Sil

    2012-04-01

    Epidemiological studies have demonstrated a possible correlation between exposure to extremely low-frequency magnetic fields (ELF-MF) and cancer. However, this correlation has yet to be definitively confirmed by epidemiological studies. The principal objective of this study was to assess the effects of 60 Hz magnetic fields in a normal cell line system, and particularly in combination with various external factors, via micronucleus (MN) assays. Mouse embryonic fibroblast NIH3T3 cells and human lung fibroblast WI-38 cells were exposed for 4 h to a 60 Hz, 1 mT uniform magnetic field with or without ionizing radiation (IR, 2 Gy), H(2)O(2) (100 μM) and cellular myelocytomatosis oncogene (c-Myc) activation. The results obtained showed no significant differences between the cells exposed to ELF-MF alone and the unexposed cells. Moreover, no synergistic effects were observed when ELF-MF was combined with IR, H(2)O(2), and c-Myc activation. Our results demonstrate that ELF-MF did not enhance MN frequency by IR, H(2)O(2) and c-Myc activation.

  19. Mitochondrial structure, function and dynamics are temporally controlled by c-Myc.

    Directory of Open Access Journals (Sweden)

    J Anthony Graves

    Full Text Available Although the c-Myc (Myc oncoprotein controls mitochondrial biogenesis and multiple enzymes involved in oxidative phosphorylation (OXPHOS, the coordination of these events and the mechanistic underpinnings of their regulation remain largely unexplored. We show here that re-expression of Myc in myc-/- fibroblasts is accompanied by a gradual accumulation of mitochondrial biomass and by increases in membrane polarization and mitochondrial fusion. A correction of OXPHOS deficiency is also seen, although structural abnormalities in electron transport chain complexes (ETC are not entirely normalized. Conversely, the down-regulation of Myc leads to a gradual decrease in mitochondrial mass and a more rapid loss of fusion and membrane potential. Increases in the levels of proteins specifically involved in mitochondrial fission and fusion support the idea that Myc affects mitochondrial mass by influencing both of these processes, albeit favoring the latter. The ETC defects that persist following Myc restoration may represent metabolic adaptations, as mitochondrial function is re-directed away from producing ATP to providing a source of metabolic precursors demanded by the transformed cell.

  20. Induction of Pluripotency in Adult Equine Fibroblasts without c-MYC

    Directory of Open Access Journals (Sweden)

    Khodadad Khodadadi

    2012-01-01

    Full Text Available Despite tremendous efforts on isolation of pluripotent equine embryonic stem (ES cells, to date there are few reports about successful isolation of ESCs and no report of in vivo differentiation of this important companion species. We report the induction of pluripotency in adult equine fibroblasts via retroviral transduction with three transcription factors using OCT4, SOX2, and KLF4 in the absence of c-MYC. The cell lines were maintained beyond 27 passages (more than 11 months and characterized. The equine iPS (EiPS cells stained positive for alkaline phosphatase by histochemical staining and expressed OCT4, NANOG, SSEA1, and SSEA4. Gene expression analysis of the cells showed the expression of OCT4, SOX2 NANOG, and STAT3. The cell lines retained a euploid chromosome count of 64 after long-term culture cryopreservation. The EiPS demonstrated differentiation capacity for the three embryonic germ layers both in vitro by embryoid bodies (EBs formation and in vivo by teratoma formation. In conclusion, we report the derivation of iPS cells from equine adult fibroblasts and long-term maintenance using either of the three reprogramming factors.

  1. Profiling and bioinformatic analysis of circular RNA expression regulated by c-Myc.

    Science.gov (United States)

    Gou, Qiheng; Wu, Ke; Zhou, Jian-Kang; Xie, Yuxin; Liu, Lunxu; Peng, Yong

    2017-09-22

    The c-Myc transcription factor is involved in cell proliferation, cell cycle and apoptosis by activating or repressing transcription of multiple genes. Circular RNAs (circRNAs) are widely expressed non-coding RNAs participating in the regulation of gene expression. Using a high-throughput microarray assay, we showed that Myc regulates the expression of certain circRNAs. A total of 309 up- and 252 down-regulated circRNAs were identified. Among them, randomly selected 8 circRNAs were confirmed by real-time PCR. Subsequently, Myc-binding sites were found to generally exist in the promoter regions of differentially expressed circRNAs. Based on miRNA sponge mechanism, we constructed circRNAs/miRNAs network regulated by Myc, suggesting that circRNAs may widely regulate protein expression through miRNA sponge mechanism. Lastly, we took advantage of Gene Ontology and KEGG analyses to point out that Myc-regulated circRNAs could impact cell proliferation through affecting Ras signaling pathway and pathways in cancer. Our study for the first time demonstrated that Myc transcription factor regulates the expression of circRNAs, adding a novel component of the Myc tumorigenic program and opening a window to investigate the function of certain circRNAs in tumorigenesis.

  2. China-United States Productivity Catch-Up

    DEFF Research Database (Denmark)

    Deng, Paul Duo; Jefferson, Gary H.

    China’s gap in industrial labor productivity with the United States has been steadily shrinking over recent decades. In this paper we examine the main sources of gap reduction and the potential for further catch-up. Using Chinese above-scale firm-level data during 1998-2007 period and BEA industry...... -level data in the US, we first document the respective rates of growth of labor productivity, gap reduction, and contributions to overall catch-up of China’s manufacturing sector during 1998-2007. We then aggregate the firm-level data to the 3-digit industry level to estimate a productivity gap...... reduction function and find that the key drivers for the productivity convergence are the initial technology gap, increased R&D spending, firm’s ownership restructuring, and industry level entry-exit ratio, a measure of competitive dynamism. A key finding is that the catch-up dynamic entails the break out...

  3. c-Myc Represses Transcription of Epstein-Barr Virus Latent Membrane Protein 1 Early after Primary B Cell Infection.

    Science.gov (United States)

    Price, Alexander M; Messinger, Joshua E; Luftig, Micah A

    2018-01-15

    Recent evidence has shown that the Epstein-Barr virus (EBV) oncogene LMP1 is not expressed at high levels early after EBV infection of primary B cells, despite its being essential for the long-term outgrowth of immortalized lymphoblastoid cell lines (LCLs). In this study, we found that expression of LMP1 increased 50-fold between 7 days postinfection and the LCL state. Metabolic labeling of nascent transcribed mRNA indicated that this was primarily a transcription-mediated event. EBNA2, the key viral transcription factor regulating LMP1, and CTCF, an important chromatin insulator, were recruited to the LMP1 locus similarly early and late after infection. However, the activating histone H3K9Ac mark was enriched at the LMP1 promoter in LCLs relative to that in infected B cells early after infection. We found that high c-Myc activity in EBV-infected lymphoma cells as well as overexpression of c-Myc in an LCL model system repressed LMP1 transcription. Finally, we found that chemical inhibition of c-Myc both in LCLs and early after primary B cell infection increased LMP1 expression. These data support a model in which high levels of endogenous c-Myc activity induced early after primary B cell infection directly repress LMP1 transcription. IMPORTANCE EBV is a highly successful pathogen that latently infects more than 90% of adults worldwide and is also causally associated with a number of B cell malignancies. During the latent life cycle, EBV expresses a set of viral oncoproteins and noncoding RNAs with the potential to promote cancer. Critical among these is the viral latent membrane protein LMP1. Prior work suggests that LMP1 is essential for EBV to immortalize B cells, but our recent work indicates that LMP1 is not produced at high levels during the first few weeks after infection. Here we show that transcription of the LMP1 gene can be negatively regulated by a host transcription factor, c-Myc. Ultimately, understanding the regulation of EBV oncogenes will allow us

  4. Modelling and forecasting monthly swordfish catches in the Eastern Mediterranean

    Directory of Open Access Journals (Sweden)

    Konstantinos I. Stergiou

    2003-04-01

    Full Text Available In this study, we used the X-11 census technique for modelling and forecasting the monthly swordfish (Xiphias gladius catches in the Greek Seas during 1982-1996 and 1997 respectively, using catches reported by the National Statistical Service of Greece (NSSG. Forecasts built with X-11 were also compared with those derived from ARIMA andWinter’s exponential smoothing (WES models. The X-11 method captured the features of the study series and outperformed the other two methods, in terms of both fitting and forecasting performance, for all the accuracy measures used. Thus, with the exception of October, November and December 1997, when the corresponding absolute percentage error(APE values were very high (as high as 178.6% because of the low level of the catches, monthly catches during the remaining months of 1997 were predicted accurately, with a mean APE of 12.5%. In contrast, the mean APE values of the other two methods for the same months were higher (ARIMA: 14.6%; WES: 16.6%. The overall good performance of X-11 andthe fact that it provides an insight into the various components (i.e. the seasonal, trend-cycle and irregular components of the time series of interest justify its use in fisheries research. The basic features of the swordfish catches revealed by the application of the X-11 method, the effect of the length of the forecasting horizon on forecasting accuracy and the accuracy of the catches reported by NSSG are also discussed.

  5. InterCatch - a tool for fish stock assessment, status and methods

    DEFF Research Database (Denmark)

    Kjems-Nielsen, Henrik; Larsen, Lena Inger; Zarecki, Maria

    2006-01-01

    InterCatch is a web-based system for handling fish stock assessment data focusing on documenting characteristics of the catches. These national fish stock data are uploaded to InterCatch by national data submitters. After all data are uploaded the stock coordinators (working for the fish stock as...... assessment group) can then check and set up allocation schemes for unsampled catches. After applying the best allocation scheme to the unsampled catches, the catch data are aggregated as required and exported for analysis, e.g. XSA or ICA....

  6. A simple statistical method for catch comparison studies

    DEFF Research Database (Denmark)

    Holst, René; Revill, Andrew

    2009-01-01

    For analysing catch comparison data, we propose a simple method based on Generalised Linear Mixed Models (GLMM) and use polynomial approximations to fit the proportions caught in the test codend. The method provides comparisons of fish catch at length by the two gears through a continuous curve...... with a realistic confidence band. We demonstrate the versatility of this method, on field data obtained from the first known testing in European waters of the Rhode Island (USA) 'Eliminator' trawl. These data are interesting as they include a range of species with different selective patterns. Crown Copyright (C...

  7. Catch of the Day, Tomorrow, and the Future

    Science.gov (United States)

    Brown, J.

    2017-12-01

    There are many fishermen and women in Pensacola, Florida, specifically at the Plaza De Luna Circle at the end of Palafox street. What are these fisherpeople hoping to catch? Do they want to eat their harvest or just catch for sport? Surveying the locals will help answer these questions and more. These answers will turn into data that will be shared and discussed with the Florida Fish and Wildlife Conservation Commission in hopes that it will give them ideas on how to manage our natural resources while best serving the interests our community.

  8. EMNE Catch-up Strategies in the Wind Turbine Industry

    DEFF Research Database (Denmark)

    Awate, Snehal; Larsen, Marcus M.; Mudambi, Ram

    2012-01-01

    EMNEs' focus on output capabilities need not facilitate innovation catch-up. We compare the knowledge bases of an industry-leading AMNE and a fast-follower EMNE using patent data, buttressed by qualitative information. The AMNE's knowledge base is deeper and composed of more distinct technology groups......Emerging economy multinationals (EMNEs) are catching up with advanced economy MNEs (AMNEs) even in emerging, high technology industries, where their knowledge-based disadvantages are most severe. We explain this phenomenon by distinguishing between output and innovation capabilities. Successful...

  9. Catch crops impact on soil water infiltration in vineyards

    Science.gov (United States)

    Cerdà, Artemi; Bagarello, Vincenzo; Iovino, Massimo; Ferro, Vito; Keesstra, Saskia; Rodrigo-Comino, Jesús; García Diaz, Andrés; di Prima, Simone

    2017-04-01

    Infiltration is the key component of the hydrological cycle (Cerdà, 1999; Bagarello et al.,, 2014; Zema et al., 2016). Infiltration determines the partitioning of rainfall into runoff and subsurface flow (Cerdà, 1996; Bagarello et al., 2006; Wang et al., 2016). In the Mediterranean, agriculture resulted in the degradation of the soil structure, reduction of the organic matter and increase in the soil losses (Cerdà et al., 2009; Laudicina et al., 2015; Iovino et al., 2016; Willaarts et al., 2016). There is an urgent need to restore the agriculture soils to avoid floods, reduce the carbon emissions and avoid reservoir siltation (Aksakal et al., 2016; Ben Slimane et al., 2016; Yagüe et al., 2016). Catch Crops are widespread used due to their impact on the soil fertility (Mwango et al., 2016; Nishigaki et al., 2016 ; Nawaz et al., 2016). Catch crops also increase the amount of organic matter but little is known about the effect on soil infiltration. Two paired plots were selected in Les Alcusses (Moixent municipality) in Eastern Iberian Peninsula to compare the infiltration rates between a 8-years catch crop (Vicia sp) with a control (plough) soil. The measurements were carried out by means of ring infiltrometer in August 2014 and December 2014 under dry and wet conditions (Cerdà, 2001; Di Prima et al., 2016). The results show that the steady-state infiltration rates were 1.8 higher during the summer period, and that the catch crops did not increase the infiltration rates. Acknowledgements The research leading to these results has received funding from the European Union Seventh Framework Programme (FP7/2007-2013) under grant agreement n 603498 (RECARE project) and the CGL2013- 47862-C2-1-R and CGL2016-75178-C2-2-R national research projects. References Aksakal, E. L., Sari, S., & Angin, I. (2016). Effects of vermicompost application on soil aggregation and certain physical properties. Land Degradation and Development, 27(4), 983-995. doi:10.1002/ldr.2350

  10. 1,25 dihydroxyvitamin D3 (1,25) regulation of c-myc mRNA in HL-60 leukemia cells

    International Nuclear Information System (INIS)

    Simpson, R.U.; Bresnick, E.H.; Begley, D.A.

    1986-01-01

    Recently, 1,25 was shown to induce differentiation and decrease c-myc levels in HL-60 cells. The authors have confirmed these observations by RNA dot blot analysis. Cells treated with 50 nM 1,25 for 4, 24 and 48 hr showed c-myc mRNA levels of 26, 17 and 15% of control respectively. β-Actin mRNA levels were not altered. To ascertain whether 1, 25 regulated c-myc transcriptionally, an HL-60 nuclear RNA runoff assay was developed. Assay of total nuclei transcriptional activity revealed that 50% of RNA elongation was α-amanitin (0.8 μg/ml) sensitive and was linear with nuclei concentration (0.1-1 x 10 7 nuclei). 1,25 (50 nM) treated (45-96 hr) cells had decreased (approx.40%) total transcription rate relative to control. Decreased total RNA synthesis occurred concomitant with NBT reducing activity. 32 P-RNA runoff transcripts from HL-60 nuclei were hybridized to excess (5 μg DNA was excess) Pst I linearized c-myc and β-actin clones (in pBR322) immobilized on nitrocellulose filter. 32 P-RNA input from 2 x 10 6 to 2 x 10 7 cpm yielded linear hybridization signal. Analysis of blot dot intensity revealed no difference in transcription of c-myc in nuclei from 1,25 dosed or control cells. (myc/actin ratios: 1,25 (50 nM, 72 hr) =1.1 +/- 0.3 and control (72 hr) = 1.0, N=3 or 2 or 3 dots ea). These preliminary data suggest 1,25 does not affect c-myc transcription in HL-60 nuclei and may regulate c-myc mRNA post-transcriptionally

  11. c-MYC G-quadruplex binding by the RNA polymerase I inhibitor BMH-21 and analogues revealed by a combined NMR and biochemical Approach.

    Science.gov (United States)

    Musso, Loana; Mazzini, Stefania; Rossini, Anna; Castagnoli, Lorenzo; Scaglioni, Leonardo; Artali, Roberto; Di Nicola, Massimo; Zunino, Franco; Dallavalle, Sabrina

    2018-03-01

    Pyridoquinazolinecarboxamides have been reported as RNA polymerase I inhibitors and represent a novel class of potential antitumor agents. BMH-21, was reported to intercalate with GC-rich rDNA, resulting in nucleolar stress as a primary mechanism of cytotoxicity. The interaction of BMH-21 and analogues with DNA G-quadruplex structures was studied by NMR and molecular modelling. The cellular response was investigated in a panel of human tumor cell lines and protein expression was examined by Western Blot analysis. We explored the ability of BMH-21 and its analogue 2 to bind to G-quadruplex present in the c-MYC promoter, by NMR and molecular modelling studies. We provide evidence that both compounds are not typical DNA intercalators but are effective binders of the tested G-quadruplex. The interaction with c-MYC G-quadruplex was reflected in down-regulation of c-Myc expression in human tumor cells. The inhibitory effect was almost complete in lymphoma cells SUDHL4 characterized by overexpression of c-Myc protein. This downregulation reflected an early and persistent modulation of cMyc mRNA. Given the relevance of c-MYC in regulation of ribosome biogenesis, it is conceivable that the inhibition of c-MYC contributes to the perturbation of nuclear functions and RNA polymerase I activity. Similar experiments with CX-5461, another RNA polymerase I transcription inhibitor, indicate the same behaviour in G-quadruplex stabilization. Our results support the hypothesis that BMH-21 and analogue compounds share the same mechanism, i.e. G-quadruplex binding as a primary event of a cascade leading to inhibition of RNA polymerase I and apoptosis. Copyright © 2017 Elsevier B.V. All rights reserved.

  12. Catching Attention in Fiber Optics Class

    OpenAIRE

    Kezerashvili, R. Ya.; Leng, L.

    2004-01-01

    Following a brief review on the history and the current development of fiber optics, the significance of teaching fiber optics for science and non-science major college students is addressed. Several experimental demonstrations designed to aid the teaching and learning process in fiber optics lectures are presented. Sample laboratory projects are also proposed to help the students to understand the physical principles of fiber optics.

  13. Catch rate of juveniles Ethamatosa fimbriata , Sardinella maderensis ...

    African Journals Online (AJOL)

    We collected data on the quantity of juvenile fish and the daily duration of fishing trips in four landing sites over a two-week period Bernoulli random variables and properties of uniform distribution were used to analyze the data. Catch rates of juveniles Ethamatosa fimbriata, Sardinella maderensis, and Brachydeuterus ...

  14. Monitoring cod catches of the Dutch demersal fleet in 2016

    NARCIS (Netherlands)

    Hal, van R.; Machiels, M.A.M.

    2017-01-01

    This report presents the results of the cod monitoring program 2016. The research was commissioned by the Dutch Ministry of Economic Affairs within the EZ-program Beleidsondersteunend Onderzoek. Cod catches of the vessels in the fleet segments BT2 (beam trawl and pulse trawl) and TR (otter trawls

  15. Comparing fish bycatch of shrimp trawlers with catches made by ...

    African Journals Online (AJOL)

    This present study identifies resource use overlap and conflict by comparing species composition, distribution patterns and abundance of bottom trawl fish bycatches and artisanal fish catches in the bay. Bottom trawl surveys were undertaken during the dry Northeast Monsoon (NEM) season and during the wet Southeast ...

  16. UNDER-REPORTING OF CATCHES OF SOUTH COAST ROCK ...

    African Journals Online (AJOL)

    The malpractice increased sharply between the 1997/98 and 2000/01 fishing seasons. The index of abundance for the resource (standardized catch per unit effort) increased by 2% for 1998/99, 12% for 1999/00 and 14% for 2000/01, after eliminating under-reported information from the input data. An agestructured ...

  17. Management of fisheries by Total Allowable Catch (TAC) regulation ...

    African Journals Online (AJOL)

    spamer

    1997) and inshore or deep-sea commercial fisheries (Patterson 1998, Hutchings and Ferguson. 2000). Treatment of ... in 2000/01 to restrict fishing effort (days at sea) on the basis of quota size and vessel efficiency. .... fleet was restricted to port after May 2001. The under- reported catch for 1998/99, when the quota was 80.1.

  18. Markov chain model for demersal fish catch analysis in Indonesia

    Science.gov (United States)

    Firdaniza; Gusriani, N.

    2018-03-01

    As an archipelagic country, Indonesia has considerable potential fishery resources. One of the fish resources that has high economic value is demersal fish. Demersal fish is a fish with a habitat in the muddy seabed. Demersal fish scattered throughout the Indonesian seas. Demersal fish production in each Indonesia’s Fisheries Management Area (FMA) varies each year. In this paper we have discussed the Markov chain model for demersal fish yield analysis throughout all Indonesia’s Fisheries Management Area. Data of demersal fish catch in every FMA in 2005-2014 was obtained from Directorate of Capture Fisheries. From this data a transition probability matrix is determined by the number of transitions from the catch that lie below the median or above the median. The Markov chain model of demersal fish catch data was an ergodic Markov chain model, so that the limiting probability of the Markov chain model can be determined. The predictive value of demersal fishing yields was obtained by calculating the combination of limiting probability with average catch results below the median and above the median. The results showed that for 2018 and long-term demersal fishing results in most of FMA were below the median value.

  19. Catch Up® Literacy: Evaluation Report and Executive Summary

    Science.gov (United States)

    Rutt, Simon; Kettlewell, Kelly; Bernardinelli, Daniele

    2015-01-01

    Catch Up® Literacy is a structured one-to-one literacy intervention for pupils between the ages of 6 and 14 who are struggling to learn to read. It teaches pupils to blend phonemes (combine letter sounds into words), segment phonemes (separate words into letter sounds), and memorise particular words so they can be understood without needing to use…

  20. Nitrogen accumulation and residual effects of nitrogen catch crops

    DEFF Research Database (Denmark)

    Jensen, E.S.

    1991-01-01

    The nitrogen accumulation in Italian ryegrass (Lolium multiflorum Lam.), perennial ryegrass (Lolium perenne L.), white mustard (Sinapis alba L.) and tansy phacelia (Phacelia tanacetifolia L.), under- or aftersown as nitrogen catch crops to spring barley (Hordeum vulgare L.) and field pea (Pisum s...

  1. 77 FR 16740 - Pacific Halibut Fisheries; Catch Sharing Plan

    Science.gov (United States)

    2012-03-22

    ...; (d) ``commercial fishing'' means fishing, the resulting catch of which is sold or bartered; or is intended to be sold or bartered, other than (i) sport fishing, (ii) treaty Indian ceremonial and... monitoring system transmitter that automatically determines a vessel's position and transmits it to a NMFS...

  2. 75 FR 13024 - Pacific Halibut Fisheries; Catch Sharing Plan

    Science.gov (United States)

    2010-03-18

    ... system for guided charter vessels (75 FR 554) was also established January 5, 2010, for Areas 2C and 3A... resulting catch of which is sold or bartered; or is intended to be sold or bartered, other than (i) sport... fish processor; (t) ``VMS transmitter'' means a NMFS-approved vessel monitoring system transmitter that...

  3. 78 FR 16423 - Pacific Halibut Fisheries; Catch Sharing Plan

    Science.gov (United States)

    2013-03-15

    ... fishing, the resulting catch of which is sold or bartered; or is intended to be sold or bartered, other... monitoring system transmitter that automatically determines a vessel's position and transmits it to a NMFS... section 13, provided that no person may sell or barter such halibut. (3) The manager of a CDQ organization...

  4. 76 FR 14300 - Pacific Halibut Fisheries; Catch Sharing Plan

    Science.gov (United States)

    2011-03-16

    ... a final rule implementing a Limited Access System for Guided Sport Charter Vessels in Alaska for... fishing, the resulting catch of which is sold or bartered; or is intended to be sold or bartered, other... monitoring system transmitter that automatically determines a vessel's position and transmits it to a NMFS...

  5. Seasonal abundance, distribution and catch per unit effort of fishes ...

    African Journals Online (AJOL)

    included a note on the ecology of the estuary and Hanekom. (1982) has completed a ... Catch per unit effort (CPUE) of fish in the Krom estuary was obtained by means ...... Structural and functional aspects of the surf-zone fish community in the ...

  6. Involvement of SIRT1 in hypoxic down-regulation of c-Myc and β-catenin and hypoxic preconditioning effect of polyphenols

    Energy Technology Data Exchange (ETDEWEB)

    Hong, Kyung-Soo [Department of Biochemistry, Pusan National University School of Medicine, Yangsan (Korea, Republic of); Research Center for Ischemic Tissue regeneration, Pusan National University School of Medicine, Yangsan (Korea, Republic of); Park, Jun-Ik [Department of Biochemistry, Pusan National University School of Medicine, Yangsan (Korea, Republic of); Kim, Mi-Ju; Kim, Hak-Bong; Lee, Jae-Won [Department of Biochemistry, Pusan National University School of Medicine, Yangsan (Korea, Republic of); Research Center for Ischemic Tissue regeneration, Pusan National University School of Medicine, Yangsan (Korea, Republic of); Dao, Trong Tuan; Oh, Won Keun [BK21 Project Team, College of Pharmacy, Chosun University, Gwangju (Korea, Republic of); Kang, Chi-Dug, E-mail: kcdshbw@pusan.ac.kr [Department of Biochemistry, Pusan National University School of Medicine, Yangsan (Korea, Republic of); Kim, Sun-Hee, E-mail: ksh7738@pusan.ac.kr [Department of Biochemistry, Pusan National University School of Medicine, Yangsan (Korea, Republic of); Research Center for Ischemic Tissue regeneration, Pusan National University School of Medicine, Yangsan (Korea, Republic of)

    2012-03-01

    SIRT1 has been found to function as a Class III deacetylase that affects the acetylation status of histones and other important cellular nonhistone proteins involved in various cellular pathways including stress responses and apoptosis. In this study, we investigated the role of SIRT1 signaling in the hypoxic down-regulations of c-Myc and β-catenin and hypoxic preconditioning effect of the red wine polyphenols such as piceatannol, myricetin, quercetin and resveratrol. We found that the expression of SIRT1 was significantly increased in hypoxia-exposed or hypoxic preconditioned HepG2 cells, which was closely associated with the up-regulation of HIF-1α and down-regulation of c-Myc and β-catenin expression via deacetylation of these proteins. In addition, blockade of SIRT1 activation using siRNA or amurensin G, a new potent SIRT1 inhibitor, abolished hypoxia-induced HIF-1α expression but increased c-Myc and β-catenin expression. SIRT1 was also found to stabilize HIF-1α protein and destabilize c-Myc, β-catenin and PHD2 under hypoxia. We also found that myricetin, quercetin, piceatannol and resveratrol up-regulated HIF-1α and down-regulated c-Myc, PHD2 and β-catenin expressions via SIRT1 activation, in a manner that mimics hypoxic preconditioning. This study provides new insights of the molecular mechanisms of hypoxic preconditioning and suggests that polyphenolic SIRT1 activators could be used to mimic hypoxic/ischemic preconditioning. -- Graphical abstract: Polyphenols mimicked hypoxic preconditioning by up-regulating HIF-1α and SIRT1 and down-regulating c-Myc, PHD2, and β-catenin. HepG2 cells were pretreated with the indicated doses of myricetin (MYR; A), quercetin (QUR; B), or piceatannol (PIC; C) for 4 h and then exposed to hypoxia for 4 h. Levels of HIF-1α, SIRT1, c-Myc, β-catenin, and PHD2 were determined by western blot analysis. The data are representative of three individual experiments. Highlights: ► SIRT1 expression is increased in hypoxia

  7. NM23-H2 may play an indirect role in transcriptional activation of c-myc gene expression but does not cleave the nuclease hypersensitive element III1

    International Nuclear Information System (INIS)

    Dexheimer, Thomas S.; Carey, Steven S.; Zuohe, Song; Gokhale, Vijay M.; Hu, Xiaohui; Murata, Lauren B.; Maes, Estelle M.; Weichsel, Andrzej; Sun, Daekyu; Meuillet, Emmanuelle J.; Montfort, William R.; Hurley, Laurence H.

    2009-01-01

    The formation of G-quadruplex structures within the nuclease hypersensitive element (NHE) III 1 region of the c-myc promoter and the ability of these structures to repress c-myc transcription have been well established. However, just how these extremely stable DNA secondary structures are transformed to activate c-myc transcription is still unknown. NM23-H2/nucleoside diphosphate kinase B has been recognized as an activator of c-myc transcription via interactions with the NHE III 1 region of the c-myc gene promoter. Through the use of RNA interference, we confirmed the transcriptional regulatory role of NM23-H2. In addition, we find that further purification of NM23-H2 results in loss of the previously identified DNA strand cleavage activity, but retention of its DNA binding activity. NM23-H2 binds to both single-stranded guanine- and cytosine-rich strands of the c-myc NHE III 1 and, to a lesser extent, to a random single-stranded DNA template. However, it does not bind to or cleave the NHE III 1 in duplex form. Significantly, potassium ions and compounds that stabilize the G-quadruplex and i-motif structures have an inhibitory effect on NM23-H2 DNA-binding activity. Mutation of Arg 88 to Ala 88 (R88A) reduced both DNA and nucleotide binding but had minimal effect on the NM23-H2 crystal structure. On the basis of these data and molecular modeling studies, we have proposed a stepwise trapping-out of the NHE III 1 region in a single-stranded form, thus allowing single-stranded transcription factors to bind and activate c-myc transcription. Furthermore, this model provides a rationale for how the stabilization of the G-quadruplex or i-motif structures formed within the c-myc gene promoter region can inhibit NM23-H2 from activating c-myc gene expression.

  8. Involvement of SIRT1 in hypoxic down-regulation of c-Myc and β-catenin and hypoxic preconditioning effect of polyphenols

    International Nuclear Information System (INIS)

    Hong, Kyung-Soo; Park, Jun-Ik; Kim, Mi-Ju; Kim, Hak-Bong; Lee, Jae-Won; Dao, Trong Tuan; Oh, Won Keun; Kang, Chi-Dug; Kim, Sun-Hee

    2012-01-01

    SIRT1 has been found to function as a Class III deacetylase that affects the acetylation status of histones and other important cellular nonhistone proteins involved in various cellular pathways including stress responses and apoptosis. In this study, we investigated the role of SIRT1 signaling in the hypoxic down-regulations of c-Myc and β-catenin and hypoxic preconditioning effect of the red wine polyphenols such as piceatannol, myricetin, quercetin and resveratrol. We found that the expression of SIRT1 was significantly increased in hypoxia-exposed or hypoxic preconditioned HepG2 cells, which was closely associated with the up-regulation of HIF-1α and down-regulation of c-Myc and β-catenin expression via deacetylation of these proteins. In addition, blockade of SIRT1 activation using siRNA or amurensin G, a new potent SIRT1 inhibitor, abolished hypoxia-induced HIF-1α expression but increased c-Myc and β-catenin expression. SIRT1 was also found to stabilize HIF-1α protein and destabilize c-Myc, β-catenin and PHD2 under hypoxia. We also found that myricetin, quercetin, piceatannol and resveratrol up-regulated HIF-1α and down-regulated c-Myc, PHD2 and β-catenin expressions via SIRT1 activation, in a manner that mimics hypoxic preconditioning. This study provides new insights of the molecular mechanisms of hypoxic preconditioning and suggests that polyphenolic SIRT1 activators could be used to mimic hypoxic/ischemic preconditioning. -- Graphical abstract: Polyphenols mimicked hypoxic preconditioning by up-regulating HIF-1α and SIRT1 and down-regulating c-Myc, PHD2, and β-catenin. HepG2 cells were pretreated with the indicated doses of myricetin (MYR; A), quercetin (QUR; B), or piceatannol (PIC; C) for 4 h and then exposed to hypoxia for 4 h. Levels of HIF-1α, SIRT1, c-Myc, β-catenin, and PHD2 were determined by western blot analysis. The data are representative of three individual experiments. Highlights: ► SIRT1 expression is increased in hypoxia

  9. Identification of a c-Jun N-terminal kinase-2-dependent signal amplification cascade that regulates c-Myc levels in ras transformation

    DEFF Research Database (Denmark)

    Mathiasen, D.P.; Egebjerg, C.; Andersen, S.H.

    2012-01-01

    are essential for ras transformation. Previous studies show that ERK-mediated serine 62 phosphorylation protects c-Myc from proteasomal degradation. ERK is, however, not alone sufficient to stabilize c-Myc but requires the cooperation of cancerous inhibitor of protein phosphatase 2A (CIP2A), an oncogene...... that counteracts protein phosphatase 2A-mediated dephosphorylation of c-Myc. Here we show that JNK2 regulates Cip2a transcription via ATF2. ATF2 and c-Myc cooperate to activate the transcription of ATF3. Remarkably, not only ectopic JNK2, but also ectopic ATF2, CIP2A, c-Myc and ATF3 are sufficient to rescue...... the defective ras transformation of JNK2-deficient cells. Thus, these data identify the key signal converging point of JNK2 and ERK pathways and underline the central role of CIP2A in ras transformation.Oncogene advance online publication, 27 June 2011; doi:10.1038/onc.2011.230....

  10. Human RNA polymerase II associated factor 1 complex promotes tumorigenesis by activating c-MYC transcription in non-small cell lung cancer

    International Nuclear Information System (INIS)

    Zhi, Xiuyi; Giroux-Leprieur, Etienne; Wislez, Marie; Hu, Mu; Zhang, Yi; Shi, Huaiyin; Du, Kaiqi; Wang, Lei

    2015-01-01

    Human RNA polymerase II (RNAPII)-associated factor 1 complex (hPAF1C) plays a crucial role in protein-coding gene transcription. Overexpression of hPAF1C has been implicated in the initiation and progression of various human cancers. However, the molecular pathways involved in tumorigenesis through hPAF1C remain to be elucidated. The current study suggested hPAF1C expression as a prognostic biomarker for early stage non-small cell lung cancer (NSCLC) and patients with low hPAF1C expression levels had significantly better overall survival. Furthermore, the expression of hPAF1C was found to be positively correlated with c-MYC expression in patient tumor samples and in cancer cell lines. Mechanistic studies indicated that hPAF1C could promote lung cancer cell proliferation through regulating c-MYC transcription. These results demonstrated the prognostic value of hPAF1C in early-stage NSCLC and the role of hPAF1C in the transcriptional regulation of c-MYC oncogene during NSCLC tumorigenesis. - Highlights: • hPAF1C expression is a prognostic biomarker for early stage non-small cell lung cancer. • The expression of hPAF1C was positively correlated with c-MYC in tumor samples of patients and in several NSCLC cell lines. • hPAF1C could promote lung cancer cell proliferation through regulating c-MYC transcription.

  11. Projectables

    DEFF Research Database (Denmark)

    Rasmussen, Troels A.; Merritt, Timothy R.

    2017-01-01

    CNC cutting machines have become essential tools for designers and architects enabling rapid prototyping, model-building and production of high quality components. Designers often cut from new materials, discarding the irregularly shaped remains. We introduce ProjecTables, a visual augmented...... reality system for interactive packing of model parts onto sheet materials. ProjecTables enables designers to (re)use scrap materials for CNC cutting that would have been previously thrown away, at the same time supporting aesthetic choices related to wood grain, avoiding surface blemishes, and other...... relevant material properties. We conducted evaluations of ProjecTables with design students from Aarhus School of Architecture, demonstrating that participants could quickly and easily place and orient model parts reducing material waste. Contextual interviews and ideation sessions led to a deeper...

  12. The selection of ultrasonic transducers for inspection of pipeline girth welds. Vol. 3. Evaluation of the pitch-catch technique for examination of the body region

    Energy Technology Data Exchange (ETDEWEB)

    Glover, A G; Fingerhut, M P; Dorling, D V

    1988-10-01

    Research was conducted to develop an ultrasonic inspection design for the nondestructive evaluation of pipeline girth welds made by the mechanized gas metal arc (GMA) welding process for onshore and offshore pipeline construction. This report describes the work carried out to evaluate the performance to the pitch-catch technique with respect to its ability to examine the body region of mechanized GMA welds in 19.5 mm thick material. Evaluation of the pitch-catch technique was carried out on simulated and real weld defects. Results show that an inspection design method and criteria can be specified for the detection of lack of sidewall fusion defects in the body region of mechanized GMA welds. The criteria specified a pitch-catch technique using a 2.25 MHz 45{degrees} transmitter and a 2.25 MHz 55{degrees} receiver probe. A single pair of these transducers can inspect wall thickness from 9.7 mm to 23.0 mm. The pitch-catch technique evaluated on 19.5 mm wall thickness materials demonstrated that the detection goal of projected depth with a signal-to-noise ratio of greater than 12dB could be met, and that no problems occurred with false indications or missed defects. High sensitivities to small defects in the body region were obtained using a single pair of pitch-catch probes that inspected the body region as a single plane. 4 refs., 14 figs., 6 tabs.

  13. Effect of fishing effort on catch rate and catchability of largemouth bass in small impoundments

    Science.gov (United States)

    Wegener, M. G.; Schramm, Harold; Neal, J. W.; Gerard, P.D.

    2018-01-01

    Largemouth bass Micropterus salmoides (Lacepède) catch rates decline with sustained fishing effort, even without harvest. It is unclear why declines in catch rate occur, and little research has been directed at how to improve catch rate. Learning has been proposed as a reason for declining catch rate, but has never been tested on largemouth bass. If catch rate declines because fish learn to avoid lures, periods of no fishing could be a management tool for increasing catch rate. In this study, six small impoundments with established fish populations were fished for two May to October fishing seasons to evaluate the effect of fishing effort on catch rate. Closed seasons were implemented to test whether a 2‐month period of no fishing improved catch rates and to determine whether conditioning from factors other than being captured reduced catch rate. Mixed‐model analysis indicated catch rate and catchability declined throughout the fishing season. Catch rate and catchability increased after a 2‐month closure but soon declined to the lowest levels of the fishing season. These changes in catch rate and catchability support the conclusion of learned angler avoidance, but sustained catchability of fish not previously caught does not support that associative or social learning affected catchability.

  14. 50 CFR 679.93 - Amendment 80 Program recordkeeping, permits, monitoring, and catch accounting.

    Science.gov (United States)

    2010-10-01

    ...) Catch accounting—(1) Amendment 80 species—(i) Amendment 80 cooperative. All Amendment 80 species caught..., permits, monitoring, and catch accounting. 679.93 Section 679.93 Wildlife and Fisheries FISHERY... recordkeeping, permits, monitoring, and catch accounting. (a) Recordkeeping and reporting. See § 679.5(s). (b...

  15. Rapid shifts in catch composition in the artisanal Red Sea reef fisheries of Eritrea

    NARCIS (Netherlands)

    Tsehaye, I.W.; Machiels, M.A.M.; Nagelkerke, L.A.J.

    2007-01-01

    Shifts in catch composition were registered in the Eritrean artisanal fisheries, which were launched into a renewed development after the end of the independence war in 1991. Our analysis of catch and effort data showed that total fishing effort as well as total annual catch increased more than

  16. Catch, effort and sampling strategies in the highly variable sardine fisheries around East Java, Indonesia.

    NARCIS (Netherlands)

    Pet, J.S.; Densen, van W.L.T.; Machiels, M.A.M.; Sukkel, M.; Setyohady, D.; Tumuljadi, A.

    1997-01-01

    Temporal and spatial patterns in the fishery for Sardinella spp. around East Java, Indonesia, were studied in an attempt to develop an efficient catch and effort sampling strategy for this highly variable fishery. The inter-annual and monthly variation in catch, effort and catch per unit of effort

  17. Regulation of c-Myc mRNA by L11 in Response to UV and Gamma Irradiation

    Science.gov (United States)

    2014-12-01

    the nucleolus fol- lowed by their transport into the cytoplasm (50). This process requires coordinated transcription catalyzed by all three RNA...these RPs, including L11, are released from the nucleolus or from intact ribosomes to suppress MDM2 (68). However, whether L11 suppresses c-Myc in...centrifugation. For isolation of the nucleolus fraction, the nuclear pellets were resuspended in buffer S1 containing 0.25 M sucrose and 10 mM MgCl2, layered over

  18. The Herpetology Project

    Science.gov (United States)

    Vacchina, Peter; Aguirre, Mary

    2008-01-01

    In this article, the authors describe their ongoing experience with the Herpetology Project. Through this field study, students catch and track amphibians and reptiles in the Assabet River area, record and analyze data, share their findings with the community, and ultimately, work toward protecting turtles' habitats. The authors chose to focus on…

  19. Synthesis of underreported small-scale fisheries catch in Pacific island waters

    Science.gov (United States)

    Zeller, D.; Harper, S.; Zylich, K.; Pauly, D.

    2015-03-01

    We synthesize fisheries catch reconstruction studies for 25 Pacific island countries, states and territories, which compare estimates of total domestic catches with officially reported catch data. We exclude data for the large-scale tuna fleets, which have largely foreign beneficial ownership, even when flying Pacific flags. However, we recognize the considerable financial contributions derived from foreign access or charter fees for Pacific host countries. The reconstructions for the 25 entities from 1950 to 2010 suggested that total domestic catches were 2.5 times the data reported to FAO. This discrepancy was largest in early periods (1950: 6.4 times), while for 2010, total catches were 1.7 times the reported data. There was a significant difference in trend between reported and reconstructed catches since 2000, with reconstructed catches declining strongly since their peak in 2000. Total catches increased from 110,000 t yr-1 in 1950 (of which 17,400 t were reported) to a peak of over 250,000 t yr-1 in 2000, before declining to around 200,000 t yr-1 by 2010. This decrease is driven by a declining artisanal (small-scale commercial) catch, which was not compensated for by increasing domestic industrial (large-scale commercial) catches. The artisanal fisheries appear to be declining from a peak of 97,000 t yr-1 in 1992 to less than 50,000 t yr-1 by 2010. However, total catches were dominated by subsistence (small-scale, non-commercial) fisheries, which accounted for 69 % of total catches, with the majority missing from the reported data. Artisanal catches accounted for 22 %, while truly domestic industrial fisheries accounted for only 6 % of total catches. The smallest component is the recreational (small-scale, non-commercial and largely for leisure) sector (2 %), which, although small in catch, is likely of economic importance in some areas due to its direct link to tourism income.

  20. In vivo distribution of c-myc antisense oligodeoxynucleotides local delivered by gelatin-coated platinmn-iridium stents in rabbits and its effect on apoptosis

    Institute of Scientific and Technical Information of China (English)

    张新霞; 崔长琮; 许香广; 胡雪松; 方卫华; 邝碧娟

    2004-01-01

    Background Post-stenting restenosis is a significant clinical problem, involving vascular smooth muscle cells(VSMCs) proliferation and apoptosis. It is reported that c-myc antisense oligodeoxynucleotides (ASODNs) local delivered by catheter can inhibit VSMCs proliferation. This study was designed to assess tissue distribution of c-myc ASODN local delivered using gelatin-coated platinum-iridium (Pt-Ir) stents, and its effect on apoptosis of VSMCs. Methods Gelatin-coated Pt-Ir stents that had absorbed caroboxyfluorescein-5-succimidyl ester (FAM) labeled c-myc ASODNs (550 μg per stent) were implanted into the right carotid arteries of 6 rabbits. Tissue samples were obtained at 45 minutes, 2 hours, and 6 hours. Tissue distribution of c- myc ASODNs was assessed by fluorescence microscopy. In addition, 32 rabbits were randomly divided into two groups. Rabbits in the control group (n=16) were implanted with gelatin-coated Pt-Ir stents, and those in the treatment group (n=16) were implanted with gelatin-coated stents that had absorbed c-myc ASODNs. 7, 14, 30, or 90 days (n=4, respectively, for each group) after the stenting procedure, the stented segments were harvested, and histopathological examinations were performed to calculate neointimal area and mean neointimal thickness. The expression of c-myc was assessed using in situ hybridization (ISH) and immunohistochemical methods. Apoptotic VSMCs were detected using terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) and transmission electron microscope (TEM). Results According to fluorescence microscopic results, FAM-labeled c-myc ASODNs were concentrated in the target vessel media at the 45 minutes time point, and then dispersed to the adventitia. Morphometric analysis showed that neointimal area and mean neointimal thickness increased continuously up to 90 days after stent implantation, but that total neointimal area and mean neointimal thickness were less in the treatment group than in the

  1. DNA repair in the c-myc proto-oncogene locus: Possible involvement in susceptibility or resistance to plasmacytoma induction in BALB/c mice

    International Nuclear Information System (INIS)

    Beecham, E.J.; Mushinski, J.F.; Shacter, E.; Potter, M.; Bohr, V.A.

    1991-01-01

    This report describes an unexpected difference in the efficiency of removal of UV-induced DNA damage in the c-myc locus in splenic B lymphoblasts from two inbred strains of mice. In cells from plasmacytoma-resistant DBA/2N mice, 35% of UV-induced damage in the regulatory and 5' flank of c-myc is removed by 12 h. However, in cells from plasmacytoma-susceptible BALB/cAn mice, damage is not removed from this region. In the protein-encoding region and 3' flank of c-myc as well as in two dihydrofolate reductase gene fragments, UV damage is repaired with similar efficiency in B lymphoblasts from both strains of mice. Furthermore, in the protein-encoding portion and 3' flank of c-myc, damage is selectively removed from only the transcribed strand. No repair is detected in the nontranscribed strand. In contrast, DNA repair in the 5' flank of c-myc is not strand specific; in DNA from DBA/2N cells, UV damage is rapidly removed from both the transcribed and nontranscribed strands. In BALB/cAn cells no repair was detected in either strand in the 5'flank, consistent with the results with double-stranded, nick-translated probes to this region of c-myc. In addition to the repair studies, we have detected post-UV-damage formation: in most of the genes studied, we find that additional T4 endonuclease-sensitive sites are formed in the DNA 2 h after irradiation. Our findings provide new insights into the details of gene-specific and strand-specific DNA repair and suggest that there may be close links between DNA repair and B-cell neoplastic development

  2. Electronic catch recordings for scientific and commercial use

    DEFF Research Database (Denmark)

    Gundersen, Agnes C.; Dyb, Jan Erik; Fossen, Inge

    For assessment and management of marine fish resources, representative data of statistically good quality describing the actual catch are lacking for many fisheries. Even for the most studied fisheries in the North Atlantic, the uncertainty regarding what is actually caught has implications...... for management. Fish stock assessments and sound advice in most cases rely on representative samples of catches. Distant and high sea fisheries often suffer from poor sampling due to sampling personal logistics. Consequently, stock assessment and management of marine fish resources exploited by those fisheries...... on the fishery, thereby meeting the challenges of obtaining representative fishery data, that is continuous and complete and willprovide sufficient data for fish stock assessments and, hence, subsequent fisheries management of species found in distant waters....

  3. Can an observer really catch up with light?

    International Nuclear Information System (INIS)

    Tian, Guihua; Zheng, Zhao

    2003-01-01

    Given a null geodesic γ 0 (λ) with a point r in (p, q) conjugate to p along γ 0 (λ), up to the second variation, there will be a variation of γ 0 (λ) which will give a timelike curve from p to q. This is the well-known theory proved in the famous book (Hawking S W and Ellis G F R 1973 The Large Scale Structure of Space-Time (Cambridge: Cambridge University Press)). In this paper, we prove that the timelike curves coming from the above-mentioned second variation have a proper acceleration approaching infinity as the timelike curves approach the null geodesic. This means no observer infinitesimally near the light can begin at the same point with it and finally catch up with it. Only when separated from the light path finitely, can the observer begin at the same point with it and really catch up with it

  4. Inhibition of c-Myc by 10058-F4 induces growth arrest and chemosensitivity in pancreatic ductal adenocarcinoma.

    Science.gov (United States)

    Zhang, Meng; Fan, Hai-Yan; Li, Sheng-Chao

    2015-07-01

    Pancreatic ductal adenocarcinoma (PDAC) is a formidable medical challenge due to its malignancies and the absence of effective treatment. c-Myc, as an important transcription factor, plays crucial roles in cell cycle progression, apoptosis and cellular transformation. The c-Myc inhibitor, 10058-F4, has been reported act as a tumor suppressor in several different tumors. In current study, the tumor-suppressive roles of 10058-F4 was observed in human pancreatic cancer cells in vitro as demonstrated by decreased cell viability, cell cycle arrest at the G1/S transition and increased caspase3/7 activity. And tumor responses to gemcitabine were also significantly enhanced by 10058-F4 in PANC-1 and SW1990 cells. In a subcutaneous xenograft model, however, 10058-F4 showed no significant influence on pancreatic tumorigenesis. When combined with gemcitabine, tumorigenesis was drastically attenuated compared with gemcitabine group or 10058-F4 group; this synergistic effect was accompanied with decreased PCNA-positive cells and reduced TUNEL-positive cells in the combined treated group. Subsequent studies revealed that decreased glycolysis may be involved in the inhibitory effect of 10058-F4 on PDAC. Taken together, this study demonstrates the roles of 10058-F4 in PDAC and provides evidence that 10058-F4 in combination with gemcitabine showed significant clinical benefit over the usage of gemcitabine alone. Copyright © 2015. Published by Elsevier Masson SAS.

  5. The c-Myc target glycoprotein1balpha links cytokinesis failure to oncogenic signal transduction pathways in cultured human cells.

    Directory of Open Access Journals (Sweden)

    Qian Wu

    2010-05-01

    Full Text Available An increase in chromosome number, or polyploidization, is associated with a variety of biological changes including breeding of cereal crops and flowers, terminal differentiation of specialized cells such as megakaryocytes, cellular stress and oncogenic transformation. Yet it remains unclear how cells tolerate the major changes in gene expression, chromatin organization and chromosome segregation that invariably accompany polyploidization. We show here that cancer cells can initiate increases in chromosome number by inhibiting cell division through activation of glycoprotein1b alpha (GpIbalpha, a component of the c-Myc signaling pathway. We are able to recapitulate cytokinesis failure in primary cells by overexpression of GpIbalpha in a p53-deficient background. GpIbalpha was found to localize to the cleavage furrow by microscopy analysis and, when overexpressed, to interfere with assembly of the cellular cortical contraction apparatus and normal division. These results indicate that cytokinesis failure and tetraploidy in cancer cells are directly linked to cellular hyperproliferation via c-Myc induced overexpression of GpIbalpha.

  6. Industrial Catching Up in the Poor Periphery 1870-1975

    OpenAIRE

    Jeffrey G. Williamson

    2011-01-01

    This paper documents industrial output and labor productivity growth around the poor periphery 1870-1975 (Latin America, the European periphery, the Middle East, South Asia, Southeast Asia and East Asia). Intensive and extensive industrial growth accelerated there over this critical century. The precocious poor periphery leaders underwent a surge and more poor countries joined their club. Furthermore, by the interwar the majority were catching up on Germany, the US and the UK, a process that ...

  7. Playing catch with energy between two superconducting coils

    International Nuclear Information System (INIS)

    Masuda, Masayoshi; Shintomi, Takakazu; Asaji, Kiyoyuki.

    1979-03-01

    The first performance of playing catch with energy between two 100 kJ superconducting magnets has been presented. The mechanism of the energy transfer as an interface between the superconducting coils is a thyristorized DC-AC-DC converter. The obtained experimental efficiency of energy transfer has been compared with the theory and good agreement has been obtained. The method will offer a versatile extension of superconductive technique in energy problems. (author)

  8. Targeted sequencing of large genomic regions with CATCH-Seq.

    Directory of Open Access Journals (Sweden)

    Kenneth Day

    Full Text Available Current target enrichment systems for large-scale next-generation sequencing typically require synthetic oligonucleotides used as capture reagents to isolate sequences of interest. The majority of target enrichment reagents are focused on gene coding regions or promoters en masse. Here we introduce development of a customizable targeted capture system using biotinylated RNA probe baits transcribed from sheared bacterial artificial chromosome clone templates that enables capture of large, contiguous blocks of the genome for sequencing applications. This clone adapted template capture hybridization sequencing (CATCH-Seq procedure can be used to capture both coding and non-coding regions of a gene, and resolve the boundaries of copy number variations within a genomic target site. Furthermore, libraries constructed with methylated adapters prior to solution hybridization also enable targeted bisulfite sequencing. We applied CATCH-Seq to diverse targets ranging in size from 125 kb to 3.5 Mb. Our approach provides a simple and cost effective alternative to other capture platforms because of template-based, enzymatic probe synthesis and the lack of oligonucleotide design costs. Given its similarity in procedure, CATCH-Seq can also be performed in parallel with commercial systems.

  9. Catch history of ringed seals (Phoca hispida in Canada

    Directory of Open Access Journals (Sweden)

    Randall R Reeves

    1998-06-01

    Full Text Available The ringed seal (Phoca hispida has always been a staple in the diet and household economy of Inuit in Canada. The present paper was prepared at the request of the NAMMCO Scientific Committee to support their assessment of ringed seal stocks in the North Atlantic Basin and adjacent arctic and subarctic waters. Specifically, our objective was to evaluate recent and current levels of use of ringed seals by Canadian Inuit. Annual removals probably were highest (possibly greater than 100,000 in the 1960s and 1970s, a period when sealskin prices were particularly strong. Catches declined substantially in the 1980s following a collapse in sealskin prices, presumably related to the European trade ban on skins from newborn harp and hooded seals (Phoca groenlandica and Cystophora cristata, respectively. Recent catch levels throughout Canada (1980s and early 1990s are believed to be in the order of 50,000 to 65,000 ringed seals, with a total average annual kill (including hunting loss in the high tens of thousands. No reliable system is in place to monitor catches of ringed seals, so any estimate must be derived from a heterogeneous array of sources.

  10. Estimating historical eastern North Pacific blue whale catches using spatial calling patterns.

    Directory of Open Access Journals (Sweden)

    Cole C Monnahan

    Full Text Available Blue whales (Balaenoptera musculus were exploited extensively around the world and remain endangered. In the North Pacific their population structure is unclear and current status unknown, with the exception of a well-studied eastern North Pacific (ENP population. Despite existing abundance estimates for the ENP population, it is difficult to estimate pre-exploitation abundance levels and gauge their recovery because historical catches of the ENP population are difficult to separate from catches of other populations in the North Pacific. We collated previously unreported Soviet catches and combined these with known catches to form the most current estimates of North Pacific blue whale catches. We split these conflated catches using recorded acoustic calls from throughout the North Pacific, the knowledge that the ENP population produces a different call than blue whales in the western North Pacific (WNP. The catches were split by estimating spatiotemporal occurrence of blue whales with generalized additive models fitted to acoustic call patterns, which predict the probability a catch belonged to the ENP population based on the proportion of calls of each population recorded by latitude, longitude, and month. When applied to the conflated historical catches, which totaled 9,773, we estimate that ENP blue whale catches totaled 3,411 (95% range 2,593 to 4,114 from 1905-1971, and amounted to 35% (95% range 27% to 42% of all catches in the North Pacific. Thus most catches in the North Pacific were for WNP blue whales, totaling 6,362 (95% range 5,659 to 7,180. The uncertainty in the acoustic data influence the results substantially more than uncertainty in catch locations and dates, but the results are fairly insensitive to the ecological assumptions made in the analysis. The results of this study provide information for future studies investigating the recovery of these populations and the impact of continuing and future sources of anthropogenic

  11. Mechanisms for c-myc Induced Mouse Mammary Gland Carcinogenesis and for the Synergistic Role of TGF(alpha) in the Process

    Science.gov (United States)

    2001-07-01

    1242 11-28. anti-tumor effects with microencapsulated c-myc antisense Panico L, D’Antonio A, Salvatore G, Mezza E, Tortora G, De oligonucleotide... enzymatic conversion of androgens to estrogens, since an estrogen receptor antagonist cannot block the lobular- alveolar induction by T, DHT

  12. Course of c-myc mRNA expression in the regenerating mouse testis determined by competitive reverse transcriptase polymerase chain reaction.

    Science.gov (United States)

    Amendola, R

    1994-11-01

    The c-myc proto-oncogene is a reliable marker of the "G0-early G1" transition, and its down-regulation is believed to be necessary to obtain cellular differentiation. In murine spermatogenesis, the level of c-myc transcripts does not correlate with the rate of cellular division. Proliferation of supposed staminal spermatogonia to reproduce themselves is induced with a local 5 Gy X-ray dose in 90-day-old C57Bl/6 mice. c-myc quantification by a newly developed competitive reverse transcriptase polymerase chain reaction (RT-PCR) was carried out to follow the expression course of this proto-oncogene. Damage and restoration of spermatogenesis were analyzed at days 3, 6, 9, 10, 13, 30, and 60 after injury by relative testes/body weight determination and histological examination. Proliferative status was determined by histone H3 Northern blot analysis. c-myc mRNA level was 10 times higher after 3 days in the irradiated animals compared to the controls. An increasing number of copies were noted up to 10 days, but promptly decreased to the base level found for irradiated mice from 13 to 60 days. Interestingly, the expression of histone H3 detected S phase only in testes at 60 days from damage.

  13. Multiple fractions of gamma rays do not induce overexpression of c-myc or c-Ki-ras oncogenes in human cervical carcinoma cells

    International Nuclear Information System (INIS)

    Osmak, M.; Soric, J.; Matulic, M.

    1993-01-01

    Multiple fractions of gamma rays (0.5 Gy daily, 30 fractions) had previously been found to change the sensitivity of human cervical carcinoma HeLa cells to anticancer drugs. Preirradiated cells became resistant to cisplatin, methotrexate and vincristine but retained the same sensitivity to gamma rays and ultraviolet light. Some mechanisms involved in the resistance of preirradiated cells to cisplatin and vincristine were determined, i.e. the increased levels of metallothioneins and increased expression of plasma membrane P glycoprotein. As recent reports indicated that the resistance to cisplatin and ionizing radiation may involve the expression of oncogenes, the problem was studied whether multiple fractions of gamma rays can change the expression of c-myc and c-Ki-ras oncogenes in HeLa cells and whether there is a correlation between the expression of these oncogenes and the sensitivity of preirradiated cells to cisplatin and gamma rays. The expression of c-myc and c-Ki-ras oncogenes was examined using the DNA dot blot, the RNA dot blot and Northern blot analysis. The results show that preirradiation induced neither amplification nor elevated expression of c-myc and c-Ki-ras oncogenes. Furthermore, there is no correlation between the expression of c-myc and c-Ki-ras oncogenes and the acquired resistance to cisplatin. (author) 3 figs., 32 refs

  14. [Relationship between the expression of beta-cat, cyclin D1 and c-myc and the occurance and biological behavior of pancreatic cancer].

    Science.gov (United States)

    Li, Yu-jun; Ji, Xiang-rui

    2003-06-01

    To study the relationship between the abnormal expression of beta-catenin (beta-cat) and the high expressions of cyclin D1 and c-myc and the occurance, proliferation, infiltration, metastasis and prognosis of pancreatic cancer, and to provide rational basis for the clinical diagnosis and treatment. Immunohistochemical PicTure trade mark was used to examine the expressions of beta-cat, cyclin D1 and c-myc in 47 cases of the cancerous tissue of pancreas, 12 cases of the pancreatic intraepithelial neoplasia and 10 cases of normal tissue of pancreas, respectively. Pancreatic cancer proliferation cell nuclear antigen (PCNA) was also tested as the index of the extent of proliferation of the pancreatic cancer. beta-cat was expressed normally in the 10 cases of the normal pancreatic tissue, while cyclin D1 and c-myc were negative. The expression rates of beta-cat, cyclin D1 and c-myc in the tissues of the pancreatic intraepithelial neoplasia and the pancreatic cancer had no significant difference [6/12 and 68.1% (32/47), 6/12 and 74.5% (35/47), 5/12 and 70.2% (33/47) respectively;P values were all more than 0.05]. The abnormal expression rate of beta-cat was significantly correlated to the metastasis of the pancreatic cancer and the one-year survival rate (both P 0.05). The expression rate of cyclin D1 was correlated with the proliferation of the pancreatic cancer and the extent of differentiation (both P 0.05). The expression rate of c-myc was not correlated with the size, the extent of proliferation, infiltration, metastasis, or one-year survival rate (both P > 0.05), but closely with the proliferation activity of the cancerous tissue of pancreas (P < 0.05). The abnormal expression of beta-cat and the high expressions of cyclin D1 and c-myc had a parallel relationship with the pancreatic intraepithelial neoplasia and pancreatic cancer (both P < 0.05, gamma = 1.000, 0.845, 0.437, 0.452). The abnormal expression of beta-cat activates cyclin D1 and c-myc, and results in the

  15. Silencing c-Myc translation as a therapeutic strategy through targeting PI3Kδ and CK1ε in hematological malignancies.

    Science.gov (United States)

    Deng, Changchun; Lipstein, Mark R; Scotto, Luigi; Jirau Serrano, Xavier O; Mangone, Michael A; Li, Shirong; Vendome, Jeremie; Hao, Yun; Xu, Xiaoming; Deng, Shi-Xian; Realubit, Ronald B; Tatonetti, Nicholas P; Karan, Charles; Lentzsch, Suzanne; Fruman, David A; Honig, Barry; Landry, Donald W; O'Connor, Owen A

    2017-01-05

    Phosphoinositide 3-kinase (PI3K) and the proteasome pathway are both involved in activating the mechanistic target of rapamycin (mTOR). Because mTOR signaling is required for initiation of messenger RNA translation, we hypothesized that cotargeting the PI3K and proteasome pathways might synergistically inhibit translation of c-Myc. We found that a novel PI3K δ isoform inhibitor TGR-1202, but not the approved PI3Kδ inhibitor idelalisib, was highly synergistic with the proteasome inhibitor carfilzomib in lymphoma, leukemia, and myeloma cell lines and primary lymphoma and leukemia cells. TGR-1202 and carfilzomib (TC) synergistically inhibited phosphorylation of the eukaryotic translation initiation factor 4E (eIF4E)-binding protein 1 (4E-BP1), leading to suppression of c-Myc translation and silencing of c-Myc-dependent transcription. The synergistic cytotoxicity of TC was rescued by overexpression of eIF4E or c-Myc. TGR-1202, but not other PI3Kδ inhibitors, inhibited casein kinase-1 ε (CK1ε). Targeting CK1ε using a selective chemical inhibitor or short hairpin RNA complements the effects of idelalisib, as a single agent or in combination with carfilzomib, in repressing phosphorylation of 4E-BP1 and the protein level of c-Myc. These results suggest that TGR-1202 is a dual PI3Kδ/CK1ε inhibitor, which may in part explain the clinical activity of TGR-1202 in aggressive lymphoma not found with idelalisib. Targeting CK1ε should become an integral part of therapeutic strategies targeting translation of oncogenes such as c-Myc. © 2017 by The American Society of Hematology.

  16. High levels of stable p53 protein and the expression of c-myc in cultured human epithelial tissue after cobalt-60 irradiation

    International Nuclear Information System (INIS)

    Mothersill, C.; Seymour, C.B.; Harney, J.; Hennessy, T.P.

    1994-01-01

    When explants of human uroepithelium or esophageal epithelium are exposed to acute doses of radiation (cobalt-60), the cells which grow out to form the primary cultures show a number of abnormal features. These include the development of characteristic nonsenescent foci. These foci have previously been shown to be c-myc positive and to have an abnormal, tumor-like ultrastructure. Expression of c-myc and the level of stable p53 proteins have now been examined in these cultures 2 weeks after irradiation. Both proteins occurred in dividing cells at the growing edge of the explant and in the foci. The expression of c-myc appeared to be correlated with growth. As expected, variation between individual cultures of normal human cells was noted in the expression of stable p53 protein. Most control uroepithelial cell cultures were negative, but a small cohort showed a wide range of values. The control cultures from the esophageal tissues had high expression of p53, and this decreased marginally after irradiation. Cells positive for p53 were always in cycle and were usually positive for c-myc as well. It would appear from these results that the expression of c-myc and the stable form of the p53 protein occur in irradiated primary cultures of normal human cells both in foci which also express a number of abnormalities and in open-quotes edgeclose quotes cells which are dividing. Cultures of unirradiated cells from esophagus and a small number of uroepithelial samples had high levels of p53. Possible reasons for this are discussed. 33 refs., 2 figs., 3 tabs

  17. Digital Health in Francophone Sub-Saharan Africa: Catching Up!

    Science.gov (United States)

    Lepère, P; Tchounga, B; Ekouevi, D-K

    2017-11-01

    Digital health has the potential to strengthen health systems and empower patients to prevent ill health and manage their own care. To confirm this potential, however, it is urgent to shift from pilot studies to the implementation of programs at a sufficient scale, with interoperable solutions and integrated into the national health system, while respecting human rights. It is also important to plan for studies to demonstrate the impact and produce the necessary evidence. Francophone sub-Saharan Africa can catch up in this area.

  18. Nm23-M2/NDP kinase B induces endogenous c-myc and nm23-M1/NDP kinase A overexpression in BAF3 cells. Both NDP kinases protect the cells from oxidative stress-induced death

    International Nuclear Information System (INIS)

    Arnaud-Dabernat, Sandrine; Masse, Karine; Smani, Moneim; Peuchant, Evelyne; Landry, Marc; Bourbon, Pierre-Marie; Le Floch, Renaud; Daniel, Jean-Yves; Larou, Monique

    2004-01-01

    The nm23 gene family encodes nucleoside diphosphate kinases (NDPKs) which supply the cell with (d)NTPs. The human NDPKB, also known as the PuF protein, binds the c-myc promoter and transactivates the c-myc protooncogene. We have now studied the effects of mouse NDPKA and NDPKB overexpression on endogenous c-myc transactivation in the mouse BAF3 and the rat PC12 cell lines. c-myc transcripts were found to be up-regulated by NDPKB only in the BAF3 line. This suggests that c-myc transcriptional control via NDPKB depends on the presence of cell-specific co-factors. Unexpectedly, NDPKB also induced NDPKA expression. This new effect was found in both cell lines, suggesting that NDPKB-dependent nm23-M1 gene transactivation requires cis and/or trans elements different from those involved in c-myc transactivation. Moreover, the BAF3 cell proliferation capacities were found to be independent of NDPKA or B cell contents. Interestingly, cell death induced by c-myc overexpression or H 2 O 2 exposure was decreased in nm23-transfected compared to control BAF3 cells. These data collectively suggest that NDPKs might improve cell survival by a mechanism coupling DNA repair and transcriptional regulation of genes involved in DNA damage response

  19. Wnt and Notch signaling pathway involved in wound healing by targeting c-Myc and Hes1 separately.

    Science.gov (United States)

    Shi, Yan; Shu, Bin; Yang, Ronghua; Xu, Yingbin; Xing, Bangrong; Liu, Jian; Chen, Lei; Qi, Shaohai; Liu, Xusheng; Wang, Peng; Tang, Jinming; Xie, Julin

    2015-06-16

    Wnt and Notch signaling pathways are critically involved in relative cell fate decisions within the development of cutaneous tissues. Moreover, several studies identified the above two pathways as having a significant role during wound healing. However, their biological effects during cutaneous tissues repair are unclear. We employed a self-controlled model (Sprague-Dawley rats with full-thickness skin wounds) to observe the action and effect of Wnt/β-catenin and Notch signalings in vivo. The quality of wound repair relevant to the gain/loss-of-function Wnt/β-catenin and Notch activation was estimated by hematoxylin-and-eosin and Masson staining. Immunofluorescence analysis and Western blot analysis were used to elucidate the underlying mechanism of the regulation of Wnt and Notch signaling pathways in wound healing. Meanwhile, epidermal stem cells (ESCs) were cultured in keratinocyte serum-free medium with Jaggedl or in DAPT (N-[(3,5-difluorophenyl)acetyl]-L-alanyl-2-phenyl]glycine-1,1-dimethylethyl) to investigate whether the interruption of Notch signaling contributes to the expression of Wnt/β-catenin signaling. The results showed that in vivo the gain-of-function Wnt/β-catenin and Notch activation extended the ability to promote wound closure. We further determined that activation or inhibition of Wnt signaling and Notch signaling can affect the proliferation of ESCs, the differentiation and migration of keratinocytes, and follicle regeneration by targeting c-Myc and Hes1, which ultimately lead to enhanced or delayed wound healing. Furthermore, Western blot analysis suggested that the two pathways might interact in vivo and in vitro. These results suggest that Wnt and Notch signalings play important roles in cutaneous repair by targeting c-Myc and Hes1 separately. What's more, interaction between the above two pathways might act as a vital role in regulation of wound healing.

  20. The TLR3/TICAM-1 signal constitutively controls spontaneous polyposis through suppression of c-Myc in Apc Min/+ mice.

    Science.gov (United States)

    Ono, Junya; Shime, Hiroaki; Takaki, Hiromi; Takashima, Ken; Funami, Kenji; Yoshida, Sumito; Takeda, Yohei; Matsumoto, Misako; Kasahara, Masanori; Seya, Tsukasa

    2017-10-17

    Intestinal tumorigenesis is promoted by myeloid differentiation primary response gene 88 (MyD88) activation in response to the components of microbiota in Apc Min/+ mice. Microbiota also contains double-stranded RNA (dsRNA), a ligand for TLR3, which activates the toll-like receptor adaptor molecule 1 (TICAM-1, also known as TRIF) pathway. We established Apc Min/+ Ticam1 -/- mice and their survival was compared to survival of Apc Min/+ Myd88 -/- and wild-type (WT) mice. The properties of polyps were investigated using immunofluorescence staining and RT-PCR analysis. We demonstrate that TICAM-1 is essential for suppression of polyp formation in Apc Min/+ mice. TICAM-1 knockout resulted in shorter survival of mice compared to WT mice or mice with knockout of MyD88 in the Apc Min/+ background. Polyps were more frequently formed in the distal intestine of Apc Min/+ Ticam1 -/- mice than in Apc Min/+ mice. Infiltration of immune cells such as CD11b + and CD8α + cells into the polyps was detected histologically. CD11b and CD8α mRNAs were increased in polyps of Apc Min/+ Ticam1 -/- mice compared to Apc Min/+ mice. Gene expression of inducible nitric oxide synthase (iNOS), interferon (IFN)-γ, CXCL9 and IL-12p40 was increased in polyps of Apc Min/+ Ticam1 -/- mice. mRNA and protein expression of c-Myc, a critical transcription factor for inflammation-associated polyposis, were increased in polyps of Apc Min/+ Ticam1 -/- mice. A Lactobacillus strain producing dsRNA was detected in feces of Apc Min/+ mice. These results imply that the TLR3/TICAM-1 pathway inhibits polyposis through suppression of c-Myc expression and supports long survival in Apc Min/+ mice.

  1. Acetylation of the c-MYC oncoprotein is required for cooperation with the HTLV-1 p30{sup II} accessory protein and the induction of oncogenic cellular transformation by p30{sup II}/c-MYC

    Energy Technology Data Exchange (ETDEWEB)

    Romeo, Megan M.; Ko, Bookyung; Kim, Janice; Brady, Rebecca; Heatley, Hayley C.; He, Jeffrey; Harrod, Carolyn K.; Barnett, Braden [Laboratory of Molecular Virology, Department of Biological Sciences, and The Dedman College Center for Drug Discovery, Design, and Delivery, Southern Methodist University, Dallas, TX 75275-0376 (United States); Ratner, Lee [Departments of Medicine and Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110 (United States); Lairmore, Michael D. [University of California-Davis, School of Veterinary Medicine, One Shields Avenue, Davis, CA 95618 (United States); Martinez, Ernest [Department of Biochemistry, University of California, Riverside, CA 92521 (United States); Lüscher, Bernhard [Institute of Biochemistry, Klinikum, RWTH Aachen University, Pauwelsstrasse 30, 52057 Aachen (Germany); Robson, Craig N. [Northern Institute for Cancer Research, Newcastle University, The Medical School, Newcastle upon Tyne, NE2 4HH (United Kingdom); Henriksson, Marie [Department of Microbiology, Cell and Tumor Biology, Karolinska Institutet, Stockholm (Sweden); Harrod, Robert, E-mail: rharrod@smu.edu [Laboratory of Molecular Virology, Department of Biological Sciences, and The Dedman College Center for Drug Discovery, Design, and Delivery, Southern Methodist University, Dallas, TX 75275-0376 (United States)

    2015-02-15

    The human T-cell leukemia retrovirus type-1 (HTLV-1) p30{sup II} protein is a multifunctional latency-maintenance factor that negatively regulates viral gene expression and deregulates host signaling pathways involved in aberrant T-cell growth and proliferation. We have previously demonstrated that p30{sup II} interacts with the c-MYC oncoprotein and enhances c-MYC-dependent transcriptional and oncogenic functions. However, the molecular and biochemical events that mediate the cooperation between p30{sup II} and c-MYC remain to be completely understood. Herein we demonstrate that p30{sup II} induces lysine-acetylation of the c-MYC oncoprotein. Acetylation-defective c-MYC Lys→Arg substitution mutants are impaired for oncogenic transformation with p30{sup II} in c-myc{sup −/−} HO15.19 fibroblasts. Using dual-chromatin-immunoprecipitations (dual-ChIPs), we further demonstrate that p30{sup II} is present in c-MYC-containing nucleoprotein complexes in HTLV-1-transformed HuT-102 T-lymphocytes. Moreover, p30{sup II} inhibits apoptosis in proliferating cells expressing c-MYC under conditions of genotoxic stress. These findings suggest that c-MYC-acetylation is required for the cooperation between p30{sup II}/c-MYC which could promote proviral replication and contribute to HTLV-1-induced carcinogenesis. - Highlights: • Acetylation of c-MYC is required for oncogenic transformation by HTLV-1 p30{sup II}/c-MYC. • Acetylation-defective c-MYC mutants are impaired for foci-formation by p30{sup II}/c-MYC. • The HTLV-1 p30{sup II} protein induces lysine-acetylation of c-MYC. • p30{sup II} is present in c-MYC nucleoprotein complexes in HTLV-1-transformed T-cells. • HTLV-1 p30{sup II} inhibits apoptosis in c-MYC-expressing proliferating cells.

  2. Variation in angler distribution and catch rates of stocked rainbow trout in a small reservoir

    Science.gov (United States)

    Harmon, Brian S.; Martin, Dustin R.; Chizinski, Christopher J.; Pope, Kevin L.

    2018-01-01

    We investigated the spatial and temporal relationship of catch rates and angler party location for two days following a publicly announced put-and-take stocking of rainbow trout (Oncorhynchus mykiss). Catch rates declined with time since stocking and distance from stocking. We hypothesized that opportunity for high catch rates would cause anglers to fish near the stocking location and disperse with time, however distance between angler parties and stocking was highly variable at any given time. Spatially explicit differences in catch rates can affect fishing quality. Further research could investigate the variation between angler distribution and fish distribution within a waterbody.

  3. Modulation of habitat-based conservation plans by fishery opportunity costs: a New Caledonia case study using fine-scale catch data.

    Directory of Open Access Journals (Sweden)

    Marilyn Deas

    Full Text Available Numerous threats impact coral reefs and conservation actions are urgently needed. Fast production of marine habitat maps promotes the use of habitat-only conservation plans, where a given percentage of the area of each habitat is set as conservation objectives. However, marine reserves can impact access to fishing grounds and generate opportunity costs for fishers that need to be minimized. In New Caledonia (Southwest Pacific, we used fine-scale fishery catch maps to define nineteen opportunity costs layers (expressed as biomass catch loss considering i total catches, ii target fish families, iii local marine tenure, and iv gear type. The expected lower impacts on fishery catch when using the different cost constraints were ranked according to effectiveness in decreasing the costs generated by the habitat-only scenarios. The exercise was done for two habitat maps with different thematic richness. In most cases, habitat conservation objectives remained achievable, but effectiveness varied widely between scenarios and between habitat maps. The results provide practical guidelines for coral reef conservation and management. Habitat-only scenarios can be used to initiate conservation projects with stakeholders but the costs induced by such scenarios can be lowered by up to 50-60% when detailed exhaustive fishery data are used. When using partial data, the gain would be only in the 15-25% range. The best compromises are achieved when using local data.

  4. Too Late to Vaccinate? The Incremental Benefits and Cost-effectiveness of a Delayed Catch-up Program Using the 4-Valent Human Papillomavirus Vaccine in Norway

    Science.gov (United States)

    Burger, Emily A.; Sy, Stephen; Nygård, Mari; Kristiansen, Ivar S.; Kim, Jane J.

    2015-01-01

    Background Human papillomavirus (HPV) vaccines are ideally administered before HPV exposure; therefore, catch-up programs for girls past adolescence have not been readily funded. We evaluated the benefits and cost-effectiveness of a delayed, 1-year female catch-up vaccination program in Norway. Methods We calibrated a dynamic HPV transmission model to Norwegian data and projected the costs and benefits associated with 8 HPV-related conditions while varying the upper vaccination age limit to 20, 22, 24, or 26 years. We explored the impact of vaccine protection in women with prior vaccine-targeted HPV infections, vaccine cost, coverage, and natural- and vaccine-induced immunity. Results The incremental benefits and cost-effectiveness decreased as the upper age limit for catch-up increased. Assuming a vaccine cost of $150/dose, vaccination up to age 20 years remained below Norway's willingness-to-pay threshold (approximately $83 000/quality-adjusted life year gained); extension to age 22 years was cost-effective at a lower cost per dose ($50–$75). At high levels of vaccine protection in women with prior HPV exposure, vaccinating up to age 26 years was cost-effective. Results were stable with lower coverage. Conclusions HPV vaccination catch-up programs, 5 years after routine implementation, may be warranted; however, even at low vaccine cost per dose, the cost-effectiveness of vaccinating beyond age 22 years remains uncertain. PMID:25057044

  5. A passive wireless ultrasound pitch–catch system

    International Nuclear Information System (INIS)

    Zahedi, F; Yao, J; Huang, H

    2015-01-01

    This paper exploits amplitude modulation and demodulation to achieve a passive wireless ultrasound pitch–catch system consisting of a wireless interrogator and a combination of a wireless actuator and a sensor mounted on a structure. The wireless interrogator operates in two modes, i.e. the generation and sensing modes. At the generation mode, the interrogator transmits two microwave signals; one is amplitude modulated with the ultrasound excitation signal while the other is a continuous-wave carrier signal. Once received by the wireless actuator, the amplitude modulated signal is demodulated using the carrier signal to recover the ultrasound excitation signal, which is then supplied to a piezoelectric wafer actuator for ultrasound generation. Subsequently, the interrogator is switched to the sensing mode by transmitting a carrier signal with a different frequency. Once received by the wireless sensor, this carrier signal is modulated with the ultrasound sensing signal acquired by the piezoelectric wafer sensor to produce an amplitude modulated microwave signal, which can then be wirelessly transmitted and demodulated by the interrogator to recover the original ultrasound sensing signal. The principle and implementation of the wireless ultrasound pitch–catch system as well as the data processing of the wirelessly received sensing signal are described. Experiment results validating wireless ultrasound generation and sensing from a distance of 0.5 m are presented. (paper)

  6. Catch-up growth or regression to the mean? Recovery from stunting revisited.

    Science.gov (United States)

    Cameron, Noël; Preece, Michael A; Cole, Tim J

    2005-01-01

    An important question for policy is the extent to which catch-up growth can ease the impact of early stunting. Martorell et al. (1992) showed that stunted Guatemalan infants remain stunted into adulthood, whereas Adair (1999) found appreciable catch-up growth in Filipino children from 2-12 years. Both groups defined catch-up as an inverse correlation between early height and subsequent growth, but Martorell based the correlation on height, whereas Adair used height z scores. The statistical phenomenon of regression to the mean is much like catch-up growth, an inverse correlation between initial height and later height gain. The objective of this study was to reexamine the relationship between stunting and later catch-up growth in the context of regression to the mean. The design was a theoretical analysis showing that catch-up growth is more evident based on height z scores than on height, validated using data on 495 stunted South African children seen at 2 and 5 years of age. The correlation between height at 2 and height change from 2 to 5 was small based on height (-0.11) but large and highly significant based on height z score (-0.58), providing strong evidence of catch-up growth. We argue that catch-up growth should be estimated using height z score not height and that catch-up is present only when the change in z score exceeds that predicted by regression to the mean. This leads to a compact definition of catch-up growth: if z1 and z2 are the initial and final (mean) height z scores, and r is the correlation between them, then catch-up growth for groups or individuals is given by (z2 - rz1). Copyright 2005 Wiley-Liss, Inc

  7. Changes in the composition of hunting catches in southeastern Cameroon: a promising approach for collaborative wildlife management between ecologists and local hunters

    Directory of Open Access Journals (Sweden)

    Hirokazu Yasuoka

    2015-12-01

    Full Text Available In recent years, both depletion of wild animals and declining food supply have threatened the livelihoods of people inhabiting the forests of the Congo Basin, and rendered the bushmeat trade a national and global concern. Because initial approaches to wildlife management were criticized for lacking consideration of the customary rights of local people, a variety of projects have been proposed to ensure their active participation in management initiatives. However, unfamiliar with the concepts of conservation ecology, local people have found it difficult to contribute effectively. This paper proposes an approach to monitor the status of fauna, based on the ratio of blue duikers (Philantomba monticola to medium-sized duikers (Cephalophus spp. for the total number of hunting catches (the catch B/M. Analysis of changes in the composition of hunting catches across multiple sites in southeastern Cameroon revealed the following trends: (1 without substantive human intervention, i.e., hunting pressure and forest disturbance, medium-sized duikers were densely distributed while blue duikers were sparse, so that the catch B/M was low; (2 under moderate human intervention, blue duikers became more densely distributed while the original density of medium-sized duikers was maintained, so that the catch B/M increased; (3 with extensive human intervention in specific areas, medium-sized duikers became sparsely distributed while a relatively high density of blue duikers was maintained, so that the catch B/M increased significantly and a mosaic of different compositions of duikers was formed; and (4 with extensive human intervention extending over the specific extensive area, both medium-sized and blue duikers became sparse. It appears that the catch B/M predicts changes in the status of game animals and of the background wild fauna, and is both a sufficiently reliable variable for ecologists and perceptible for local people. Furthermore, this approach has the

  8. 50 CFR 679.84 - Rockfish Program recordkeeping, permits, monitoring, and catch accounting.

    Science.gov (United States)

    2010-10-01

    ..., monitoring, and catch accounting. 679.84 Section 679.84 Wildlife and Fisheries FISHERY CONSERVATION AND..., permits, monitoring, and catch accounting. (a) Recordkeeping and reporting. See § 679.5(r). (b) Permits... cooperative or rockfish limited access fishery. The requirements under paragraphs (c)(1) through (9) of this...

  9. A Global Analysis of the Relationship between Farmed Seaweed Production and Herbivorous Fish Catch.

    Directory of Open Access Journals (Sweden)

    E James Hehre

    Full Text Available Globally, farmed seaweed production is expanding rapidly in shallow marine habitats. While seaweed farming provides vital income to millions of artisanal farmers, it can negatively impact shallow coral reef and seagrass habitats. However, seaweed farming may also potentially provide food subsidies for herbivorous reef fish such as the Siganidae, a valuable target family, resulting in increased catch. Comparisons of reef fish landings across the central Philippines revealed that the catch of siganids was positively correlated to farmed seaweed production whilst negatively correlated to total reef fish catch over the same period of time. We tested the generality of this pattern by analysing seaweed production, siganid catch, and reef fish catch for six major seaweed-producing countries in the tropics. We hypothesized that increased seaweed production would correspond with increased catch of siganids but not other reef fish species. Analysis of the global data showed a positive correlation between farmed seaweeds and siganids in Southeast Asia (Indonesia, Malaysia, and the Philippines but not Africa (Tanzania and Zanzibar, or the Western Pacific (Fiji. In Southeast Asia, siganid catch increased disproportionately faster with seaweed production than did reef fish catch. Low continuity, sporadic production and smaller volumes of seaweed farming may explain the differences.

  10. A Global Analysis of the Relationship between Farmed Seaweed Production and Herbivorous Fish Catch.

    Science.gov (United States)

    Hehre, E James; Meeuwig, Jessica J

    2016-01-01

    Globally, farmed seaweed production is expanding rapidly in shallow marine habitats. While seaweed farming provides vital income to millions of artisanal farmers, it can negatively impact shallow coral reef and seagrass habitats. However, seaweed farming may also potentially provide food subsidies for herbivorous reef fish such as the Siganidae, a valuable target family, resulting in increased catch. Comparisons of reef fish landings across the central Philippines revealed that the catch of siganids was positively correlated to farmed seaweed production whilst negatively correlated to total reef fish catch over the same period of time. We tested the generality of this pattern by analysing seaweed production, siganid catch, and reef fish catch for six major seaweed-producing countries in the tropics. We hypothesized that increased seaweed production would correspond with increased catch of siganids but not other reef fish species. Analysis of the global data showed a positive correlation between farmed seaweeds and siganids in Southeast Asia (Indonesia, Malaysia, and the Philippines) but not Africa (Tanzania and Zanzibar), or the Western Pacific (Fiji). In Southeast Asia, siganid catch increased disproportionately faster with seaweed production than did reef fish catch. Low continuity, sporadic production and smaller volumes of seaweed farming may explain the differences.

  11. Thirty years of monitoring traditional fish trap catches at Kosi Bay ...

    African Journals Online (AJOL)

    ... mark and recapture studies, suggest a very high, and possibly unsustainable, catch rate that requires management intervention to return them back to historical and sustainable levels. Keywords: catch monitoring, estuarine, fisheries, fish marking, fish traps, iSimangaliso, overfishing, South-East Africa, World Heritage Site

  12. Few data but many fish: marine small-scale fisheries catches for ...

    African Journals Online (AJOL)

    ... substantially under-reported national data puts authorities under serious risk of over-licensing fishing access and mismanaging marine ecosystems and national food security. Keywords: catch rates, catch reconstructions, food security, Malthusian overfishing, small-scale fisheries, sub-Saharan Africa, subsistence fisheries

  13. The Effect of Intellectual Property Standards on the Catch-Up Process Of Emerging Market Economies

    DEFF Research Database (Denmark)

    Darendeli, Izzet; Brandl, Kristin Martina; Hamilton, III, Robert D.

    2014-01-01

    The catch-up process of emerging market economies is dependent on multiple factors, such as local governmental regulations but also global industry developments. We investigate how intellectual property (IP) protection standards affect this catch-up process. The alignment of these standards...

  14. Tyrosine requirement during the rapid catch-up growth phase of recovery from severe childhood undernutrition

    Science.gov (United States)

    The requirement for aromatic amino acids, during the rapid catch-up in weight phase of recovery from severe childhood under nutrition (SCU) is not clearly established. As a first step, the present study aimed to estimate the tyrosine requirement of children with SCU during the catch-up growth phase ...

  15. Experiment and analysis of basic phenomena of gas catching by liquid surface. Observation by visualizing vortex structure and gas catching process

    International Nuclear Information System (INIS)

    Kamemoto, Takashi; Nishiyama, Tadao

    1995-01-01

    Since gravity, viscous force, surface tension and so on are related simultaneously to the inertia force of flow, in gas catching phenomena, it is often difficult to grasp exactly its similarity. At the time of designing actual equipment, careful model test is required, and the validity of the evaluation by model test for applying it to actual machines sometimes becomes a problem. In this research, for the purpose of elucidating the essential mechanism of the gas-catching phenomena by vortices, and obtaining the knowledge useful for the probability of the method of evaluating the limit of gas catching, the knowledge obtained so far on the similarity law and model testing method related to the air catching by vortices was put in order, and vortex structure and basic gas-catching process were observed by water flow visualizing experiment, thus the noteworthy flow characteristics for clarifying the essential mechanism of the phenomena were obtained. The main knowledges on the air catching by vortices obtained so far, the experiment of visualizing vortices using water flow and the experimental results are reported. (K.I.)

  16. TEST OF THE CATCH-UP HYPOTHESIS IN AFRICAN AGRICULTURAL GROWTH RATES

    Directory of Open Access Journals (Sweden)

    Kalu Ukpai IFEGWU

    2015-11-01

    Full Text Available The paper tested the catch-up hypothesis in agricultural growth rates of twenty-six African countries. Panel data used was drawn from the Food and Agricultural Organization Statistics (FAOSTAT of the United Nations. The Data Envelopment Analysis Method for measuring productivity was used to estimate productivity growth rates. The cross-section framework consisting of sigma-convergence and beta-convergence was employed to test the catching up process. Catching up is said to exist if the value of beta is negative and significant. Since catching up does not necessarily imply narrowing of national productivity inequalities, sigma-convergence which measures inequality, was estimated for the same variables. The results showed evidence of the catch-up process, but failed to find a narrowing of productivity inequalities among countries.

  17. Using demographic data to better interpret pitfall trap catches

    Directory of Open Access Journals (Sweden)

    Andrey Matalin

    2011-05-01

    Full Text Available The results of pitfall trapping are often interpreted as abundance in a particular habitat. At the same time, there are numerous cases of almost unrealistically high catches of ground beetles in seemingly unsuitable sites. The correlation of catches by pitfall trapping with the true distribution and abundance of Carabidae needs corroboration. During a full year survey in 2006/07 in the Lake Elton region (Volgograd Area, Russia, 175 species of ground beetles were trapped. Considering the differences in demographic structure of the local populations, and not their abundances, three groups of species were recognized: residents, migrants and sporadic. In residents, the demographic structure of local populations is complete, and their habitats can be considered “residential”. In migrants and sporadic species, the demographic structure of the local populations is incomplete, and their habitats can be considered “transit”. Residents interact both with their prey and with each other in a particular habitat. Sporadic species are hardly important to a carabid community because of their low abundances. The contribution of migrants to the structure of carabid communities is not apparent and requires additional research. Migrants and sporadic species represent a “labile” component in ground beetles communities, as opposed to a “stable” component, represented by residents. The variability of the labile component substantially limits our interpretation of species diversity in carabid communities. Thus, the criteria for determining the most abundant, or dominant species inevitably vary because the abundance of migrants in some cases can be one order of magnitude higher than that of residents. The results of pitfall trapping adequately reflect the state of carabid communities only in zonal habitats, while azonal and disturbed habitats are merely transit ones for many species of ground beetles. A study of the demographic structure of local

  18. Using demographic data to better interpret pitfall trap catches.

    Science.gov (United States)

    Matalin, Andrey V; Makarov, Kirill V

    2011-01-01

    The results of pitfall trapping are often interpreted as abundance in a particular habitat. At the same time, there are numerous cases of almost unrealistically high catches of ground beetles in seemingly unsuitable sites. The correlation of catches by pitfall trapping with the true distribution and abundance of Carabidae needs corroboration. During a full year survey in 2006/07 in the Lake Elton region (Volgograd Area, Russia), 175 species of ground beetles were trapped. Considering the differences in demographic structure of the local populations, and not their abundances, three groups of species were recognized: residents, migrants and sporadic. In residents, the demographic structure of local populations is complete, and their habitats can be considered "residential". In migrants and sporadic species, the demographic structure of the local populations is incomplete, and their habitats can be considered "transit". Residents interact both with their prey and with each other in a particular habitat. Sporadic species are hardly important to a carabid community because of their low abundances. The contribution of migrants to the structure of carabid communities is not apparent and requires additional research. Migrants and sporadic species represent a "labile" component in ground beetles communities, as opposed to a "stable" component, represented by residents. The variability of the labile component substantially limits our interpretation of species diversity in carabid communities. Thus, the criteria for determining the most abundant, or dominant species inevitably vary because the abundance of migrants in some cases can be one order of magnitude higher than that of residents. The results of pitfall trapping adequately reflect the state of carabid communities only in zonal habitats, while azonal and disturbed habitats are merely transit ones for many species of ground beetles. A study of the demographic structure of local populations and assessment of the

  19. Profil Ekspresi mRNA Gen Murine Double Minute2, Kruppel-Like Factor4, dan c-Myc pada Fibrosarkoma

    Directory of Open Access Journals (Sweden)

    - Humaryanto

    2017-02-01

    Full Text Available Abstrak Fibrosarkoma hanya terjadi 1–3% dari seluruh keganasan jaringan lunak. Hingga saat ini etiologi fibrosarkoma belum diketahui dengan pasti. Beberapa faktor dapat menjadi penyebab patogenesis fibrosarkoma antara lain radiasi, terpapar zat kimia tertentu, serta infeksi human herpes virus 8 (HHV8 dan Epstein-Barr virus (EBV. Penelitian terkini menunjukkan bahwa banyak sarkoma terkait dengan mutasi genetik. Penelitian ini bertujuan melihat profil ekspresi mRNA gen Krüppel-like Factor4, Murine Double Minute2, dan c-Myc pada fibrosarkoma menggunakan teknik real time PCR kuantitatif (quantitative real time PCR, qRT-PCR. Analisis data menggunakan metode kuantititatif relatif 2-ΔΔCt. Penelitian ini menggunakan 10 sampel kasus fibrosarkoma yang ditemukan di Kota Jambi dari tahun 2011–2015. Hasil ΔCt (+SD MDM2, KLF-4, dan c-Myc disusun dari nilai yang terkecil hingga tertinggi adalah 1,85±2,14; 2,06±3,86; 2,9±2,66 secara berurutan. Dibanding dengan level ekspresi dengan GAPDH sebagai housekeeping gene, gen MDM2 dan KLF-4 relatif menurun dua kali lipat, sedangkan gen c-Myc relatif menurun lebih dari tiga kali lipat. Simpulan, penelitian ini menunjukkan bahwa pada kasus fibrosarkoma, gen c-Myc disupresi lebih kuat dibanding dengan gen MDM2 dan KLF-4. Abstract Fibrosarcoma is a rare soft tissue sarcoma, reported only 1–3% of all soft tissue sarcomas. Like any other soft-tissue sarcomas the definitive caused has not yet understood. Recognized causes include exposure to ionizing radiation, various physical and chemical factors, infection with human herpes virus (HHV8 and Epstein-Barr virus (EBV. Current research indicates many sarcomas are associated with genetic mutations. In this study, we investigated profile of mRNA gene expression KLF4, MDM2, and c-Myc of RNA in fibrosarcoma cases. The genes expression was examined using quantitative real time PCR (qRT-PCR and we analyzed the relative gene expression using the 2-ΔΔCt method. Ten

  20. Targeting human c-Myc promoter duplex DNA with actinomycin D by use of multi-way analysis of quantum-dot-mediated fluorescence resonance energy transfer

    DEFF Research Database (Denmark)

    Gholami, Somayeh; Kompany Zare, Mohsen

    2013-01-01

    Actinomycin D (Act D), an oncogenic c-Myc promoter binder, interferes with the action of RNA polymerase. There is great demand for high-throughput technology able to monitor the activity of DNA-binding drugs. To this end, binding of 7-aminoactinomycin D (7AAD) to the duplex c-Myc promoter...... pairs resulted in efficient energy transfer from drug to QD via fluorescence resonance energy transfer (FRET). Multi-way analysis of the three-way data array obtained from titration experiments was performed by use of restricted Tucker3 and hard trilinear decomposition (HTD). These techniques enable...... the important advantage over univariate classical methods of enabling us to investigate the source of variance in the fluorescence signal of the DNA-drug complex. It was established that hard trilinear decomposition analysis of FRET-measured data overcomes the problem of rank deficiency, enabling calculation...

  1. Relationship of Amplification and Expression of the C-MYC Gene with Survival among Gastric Cancer Patients.

    Science.gov (United States)

    Khaleghian, Malihea; Shakoori, Abbas; Razavi, Amirnader Emami; Azimi, Cyrus

    2015-01-01

    During the past decades, the incidence and mortality rate of stomach cancer has demonstrated a great decrease in the world, but it is still one of the most common and fatal cancers especially among men worldwide, including Iran. The MYC proto-oncogene, which is located at 8q24.1, regulates 15% of genes and is activated in 20% of all human tumors. MYC amplification and overexpression of its protein product has been reported in 15-30% of gastric neoplasias. The aim of this investigation was to find the relative efficacy of CISH (chromogenic in situ hybridization) or IHC (immunohistochemistry) in diagnosis and prognosis of gastric cancer, as well as the relationship of amplification and expression of C-MYC gene with patient survival. In this cross-sectional study, 102 samples of gastric cancer were collected from patients who had undergone primary surgical resection at the Cancer Institute Hospital, Tehran University of Medical Sciences, from July 2009 to March 2014. All samples were randomly selected from those who were diagnosed with gastric adenocarcinomas. CISH and IHC methods were performed on all of them. Patients were classified into two groups. The first consisted of stage I and II cases, and the second of stage III and IV. Survival tests for both groups was carried out with referrnce to CISH test reults. Group II (stage III and IV) with CISH+ featured lower survival than those with CISH- (p=0.233), but group I (stage I and II) patients demonstrated no significant variation with CISH+ or CISH- (p=0.630). Kaplan-Meier for both groups was carried out with IHC test findings and showed similar results. This data revealed that both diffuse and intestinal types of gastric cancer occurred significantly more in men than women. Our data also showed that CISH+ patients (43%) were more frequent in comparison with IHC+ patients (14.7%). For planning treatment of gastric cancer patients, by focusing on expanding tumors, which is the greatest concern of the surgeons and

  2. Detecção imunoistoquímica das oncoproteínas p21ras, c-myc E p53 no carcinoma hepatocelular e no tecido hepático não-neoplásico Immunohistochemical detection of p21ras, c-myc and p53 oncoproteins in hepatocellular carcinoma and in non-neoplastic liver tissue

    Directory of Open Access Journals (Sweden)

    Vera Lucia Nunes Pannain

    2004-12-01

    Full Text Available RACIONAL: A hepatocarcinogênese é um processo no qual as alterações genéticas e epigenéticas são bem conhecidas em modelos animais, mas carece de estudos no homem. OBJETIVOS: Analisar a freqüência das oncoproteínas p21ras, c-myc e p53 no carcinoma hepatocelular e no fígado não-neoplásico. Verificar ainda a associação destas oncoproteínas com os padrões e graus histológicos, assim como com as infecções pelos vírus das hepatites B e C. MÉTODOS: Foi analisada por método imunoistoquímico a detecção das oncoproteínas p21ras, c-myc e p53 em 47 casos de carcinoma hepatocelular e no tecido não-neoplásico circunjacente ao tumor (40 casos. RESULTADOS: As oncoproteínas p21ras, c-myc e p53 foram detectadas, respectivamente, em 44,7%, 53,2% e 36,2% dos casos de carcinoma hepatocelular. A imunorreatividade do p21ras e c-myc mostrou uma associação significativa. Contudo, não houve associação significativa entre a detecção do p21ras, c-myc e p53 com os diferentes graus e padrões histológicos, nem tampouco com as infecções pelos vírus das hepatites B e C. A mesma associação significativa entre o p21ras e c-myc foi encontrada no tecido não-neoplásico dos casos de cirrose em relação aos que não apresentaram cirrose, enquanto que o p53 foi negativo em todos os casos. CONCLUSÕES: A imunorreatividade das oncoproteínas p21ras, c-myc e p53 corrobora evidências prévias de sua detecção no carcinoma hepatocelular, o que sugere poder haver participação destas proteínas na hepatocarcinogênese humana. A significativa associação entre as proteínas p21ras, c-myc e p53 no carcinoma hepatocelular e na cirrose pode apontar uma interação entre as mesmas, sobretudo na hepatocarcinogênese pela via da cirrose.BACKGROUND: Genetic and epigenetic alterations have been described in animal hepatocarcinogenesis models but need to be studied in human being. AIMS: To assess the immunoreactivity of p21ras, c-myc and p53

  3. Cytotoxic effect of γ-sitosterol from Kejibeling (Strobilanthes crispus and its mechanism of action towards c-myc gene expression and apoptotic pathway

    Directory of Open Access Journals (Sweden)

    Susi Endrini

    2015-01-01

    Full Text Available Background: This study aimed to analyze the cytotoxicity effect of γ-sitosterol isolated from “Kejibeling” (Strobilanthes crispus, a medicinal plant, on several cancer cell lines. The mechanisms of the effects were studied through the expression of cancer-caused gene, c-myc and apoptotic pathways.Methods: This in vitro study was done using human colon cancer cell lines (Caco-2, liver cancer cell lines (HepG2, hormone-dependent breast cancer cell lines (MCF-7 and the normal liver cell lines (Chang Liver. The cytotoxic effect was measured through MTT assay and the potential cytotoxic value was calculated by determining the toxic concentration which may kill up to 50% of the total cell used (IC50. Meanwhile, the cytotoxic mechanism was studied by determining the effect of adding γ-sitosterol to the c-myc gene expression by reverse transciptase-polymerase chain reaction (RT-PCR. The effect of γ-sitosterol through apoptotic pathway was studied by using terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL assay.Results: γ-sitosterol was cytotoxic against Caco-2, HepG2, and MCF-7 with IC50-values of 8.3, 21.8, and 28.8 μg/mL, respectively. There were no IC50-values obtained from this compound against Chang Liver cell line. This compound induced apotosis on Caco-2 and HepG2 cell lines and suppressed the c-myc genes expression in both cells.Conclusion: γ-sitosterol was cytotoxic against colon and liver cancer cell lines and the effect was mediated by down-regulation of c-myc expression and induction of the apoptotic pathways.

  4. Sodium arsenite alters cell cycle and MTHFR, MT1/2, and c-Myc protein levels in MCF-7 cells

    International Nuclear Information System (INIS)

    Ruiz-Ramos, Ruben; Lopez-Carrillo, Lizbeth; Albores, Arnulfo; Hernandez-Ramirez, Raul U.; Cebrian, Mariano E.

    2009-01-01

    There is limited available information on the effects of arsenic on enzymes participating in the folate cycle. Therefore, our aim was to evaluate the effects of sodium arsenite on the protein levels of methylenetetrahydrofolate reductase (MTHFR) and dihydrofolate reductase (DHFR) and its further relationship with the expression MT1/2 and c-myc in MCF-7 cells. Arsenite treatment (0-10 μM) for 4 h decreased MTHFR levels in a concentration-dependent fashion without significant effects on DHFR. The effects on MTHFR were observed at arsenite concentrations not significantly affecting cell viability. We also observed an increase in S-phase recruitment at all concentrations probed. Lower concentrations (< 5 μM) induced cell proliferation, showing a high proportion of BrdU-stained cells, indicating a higher DNA synthesis rate. However, higher concentrations (≥ 5 μM) or longer treatment periods induced apoptosis. Arsenite also induced dose-dependent increases in MT1/2 and c-Myc protein levels. The levels of MTHFR were inversely correlated to MT1/2 and c-Myc overexpression and increased S-phase recruitment. Our findings indicate that breast epithelial cells are responsive to arsenite and suggest that exposure may pose a risk for breast cancer. The reductions in MTHFR protein levels contribute to understand the mechanisms underlying the induction of genes influencing growth regulation, such as c-myc and MT1/2. However, further research is needed to ascertain if the effects here reported following short-time and high-dose exposure are relevant for human populations chronically exposed to low arsenic concentrations.

  5. Aspirin therapy reduces the ability of platelets to promote colon and pancreatic cancer cell proliferation: Implications for the oncoprotein c-MYC

    Science.gov (United States)

    Sylman, Joanna L.; Ngo, Anh T. P.; Pang, Jiaqing; Sears, Rosalie C.; Williams, Craig D.; McCarty, Owen J. T.

    2017-01-01

    Aspirin, an anti-inflammatory and antithrombotic drug, has become the focus of intense research as a potential anticancer agent owing to its ability to reduce tumor proliferation in vitro and to prevent tumorigenesis in patients. Studies have found an anticancer effect of aspirin when used in low, antiplatelet doses. However, the mechanisms through which low-dose aspirin works are poorly understood. In this study, we aimed to determine the effect of aspirin on the cross talk between platelets and cancer cells. For our study, we used two colon cancer cell lines isolated from the same donor but characterized by different metastatic potential, SW480 (nonmetastatic) and SW620 (metastatic) cancer cells, and a pancreatic cancer cell line, PANC-1 (nonmetastatic). We found that SW480 and PANC-1 cancer cell proliferation was potentiated by human platelets in a manner dependent on the upregulation and activation of the oncoprotein c-MYC. The ability of platelets to upregulate c-MYC and cancer cell proliferation was reversed by an antiplatelet concentration of aspirin. In conclusion, we show for the first time that inhibition of platelets by aspirin can affect their ability to induce cancer cell proliferation through the modulation of the c-MYC oncoprotein. PMID:27903583

  6. Regulation of c-myc and c-fos mRNA levels by polyomavirus: distinct roles for the capsid protein VP1 and the viral early proteins

    International Nuclear Information System (INIS)

    Zullo, J.; Stiles, C.D.; Garcea, R.L.

    1987-01-01

    The levels of c-myc, c-fos, and JE mRNAs accumulate in a biphasic pattern following infection of quiescent BALB/c 3T3 mouse cells with polyomavirus. Maximal levels of c-myc and c-fos mRNAs were seen within 1 hr and were nearly undetectable at 6 hr after infection. At 12 hr after infection mRNA levels were again maximal and remained elevated thereafter. Empty virions (capsids) and recombinant VP 1 protein, purified from Escherichia coli, induced the early but not the late phase of mRNA accumulation. Virions, capsids, and recombinant VP 1 protein stimulated [ 3 H]thymidine nuclear labeling and c-myc mRNA accumulation in a dose-responsive manner paralleling their affinity for the cell receptor for polyoma. The second phase of mRNA accumulation is regulated by the viral early gene products, as shown by polyomavirus early gene mutants and by a transfected cell line (336a) expressing middle tumor antigen upon glucocorticoid addition. These results suggest that polyomavirus interacts with the cell membrane at the onset of infection to increase the levels of mRNA for the cellular genes associated with cell competence for DNA replication, and subsequently these levels are maintained by the action of the early viral proteins

  7. Aspirin regulation of c-myc and cyclinD1 proteins to overcome tamoxifen resistance in estrogen receptor-positive breast cancer cells.

    Science.gov (United States)

    Cheng, Ran; Liu, Ya-Jing; Cui, Jun-Wei; Yang, Man; Liu, Xiao-Ling; Li, Peng; Wang, Zhan; Zhu, Li-Zhang; Lu, Si-Yi; Zou, Li; Wu, Xiao-Qin; Li, Yu-Xia; Zhou, You; Fang, Zheng-Yu; Wei, Wei

    2017-05-02

    Tamoxifen is still the most commonly used endocrine therapy drug for estrogen receptor (ER)-positive breast cancer patients and has an excellent outcome, but tamoxifen resistance remains a great impediment to successful treatment. Recent studies have prompted an anti-tumor effect of aspirin. Here, we demonstrated that aspirin not only inhibits the growth of ER-positive breast cancer cell line MCF-7, especially when combined with tamoxifen, but also has a potential function to overcome tamoxifen resistance in MCF-7/TAM. Aspirin combined with tamoxifen can down regulate cyclinD1 and block cell cycle in G0/G1 phase. Besides, tamoxifen alone represses c-myc, progesterone receptor (PR) and cyclinD1 in MCF-7 cell line but not in MCF-7/TAM, while aspirin combined with tamoxifen can inhibit the expression of these proteins in the resistant cell line. When knocking down c-myc in MCF-7/TAM, cells become more sensitive to tamoxifen, cell cycle is blocked as well, indicating that aspirin can regulate c-myc and cyclinD1 proteins to overcome tamoxifen resistance. Our study discovered a novel role of aspirin based on its anti-tumor effect, and put forward some kinds of possible mechanisms of tamoxifen resistance in ER-positive breast cancer cells, providing a new strategy for the treatment of ER-positive breast carcinoma.

  8. Somatostatin reduces 3H-thymidine incorporation and c-myc, but not thyroglobulin ribonucleic acid levels in human thyroid follicular cells in vitro

    International Nuclear Information System (INIS)

    degli Uberti, E.C.; Hanau, S.; Rossi, R.; Piva, R.; Margutti, A.; Trasforini, G.; Pansini, G.; del Senno, L.

    1991-01-01

    The action of somatostatin (SRIH) on 3 H-thymidine (thy) incorporation and on c-myc and thyroglobulin RNA levels in a suspension of follicles from normal and goitrous human thyroid was examined. SRIH, at 10 - 7 M concentration, inhibited basal thy incorporation (maximally by 4 h lasting for up 24 h), which effect was greater in goiter than in normal thyroid and was also detected in growing adherent epithelial cells. Moreover, in a follicle suspension SRIH prevented TSH-stimulated thy incorporation, both in normal and in goitrous thyroid. Basal expression of c-myc RNA was not affected by SRIH in either tissue, whereas the TSH-stimulated c-myc RNA level was significantly reduced in goiter. No effect of SRIH was observed on basal or TSH-stimulated thyroglobulin RNA levels. SRIH did not alter basal cAMP concentrations in normal or goitrous follicles, but it significantly reduced TSH-stimulated cAMP accumulation both in normal thyroid and in goiter. Overall, our data indicate a direct inhibitory action of SRIH on growth, but not on differentiation, of human thyroid, probably by a mechanism not entirely cAMP dependent

  9. Human amniotic epithelial cell feeder layers maintain mouse embryonic stem cell pluripotency via epigenetic regulation of the c-Myc promoter.

    Science.gov (United States)

    Liu, Te; Cheng, Weiwei; Liu, Tianjin; Guo, Lihe; Huang, Qin; Jiang, Lizhen; Du, Xiling; Xu, Fuhui; Liu, Zhixue; Lai, Dongmei

    2010-02-01

    Mouse embryonic stem cells (ESCs) are typically cultured on a feeder layer of mouse embryonic fibroblasts (MEFs), with leukemia inhibitory factor (LIF) added to maintain them in an undifferentiated state. We have previously shown that human amniotic epithelial cells (hAECs) can be used as feeder cells to maintain mouse ESC pluripotency, but the mechanism for this is unknown. In the present study, we found that CpG islands 5' of the c-Myc gene remain hypomethylated in mouse ESCs cultured on hAECs. In addition, levels of acetylation of histone H3 and trimethylation of histone H3K4 in the c-Myc gene promoter were higher in ES cells cultured on hAECs than those in ES cells cultured on MEFs. These data suggested that hAECs can alter mouse ESC gene expression via epigenetic modification of c-Myc, providing a possible mechanism for the hAEC-induced maintenance of ESCs in an undifferentiated state.

  10. The Dysregulation of Polyamine Metabolism in Colorectal Cancer Is Associated with Overexpression of c-Myc and C/EBPβ rather than Enterotoxigenic Bacteroides fragilis Infection

    Directory of Open Access Journals (Sweden)

    Anastasiya V. Snezhkina

    2016-01-01

    Full Text Available Colorectal cancer is one of the most common cancers in the world. It is well known that the chronic inflammation can promote the progression of colorectal cancer (CRC. Recently, a number of studies revealed a potential association between colorectal inflammation, cancer progression, and infection caused by enterotoxigenic Bacteroides fragilis (ETBF. Bacterial enterotoxin activates spermine oxidase (SMO, which produces spermidine and H2O2 as byproducts of polyamine catabolism, which, in turn, enhances inflammation and tissue injury. Using qPCR analysis, we estimated the expression of SMOX gene and ETBF colonization in CRC patients. We found no statistically significant associations between them. Then we selected genes involved in polyamine metabolism, metabolic reprogramming, and inflammation regulation and estimated their expression in CRC. We observed overexpression of SMOX, ODC1, SRM, SMS, MTAP, c-Myc, C/EBPβ (CREBP, and other genes. We found that two mediators of metabolic reprogramming, inflammation, and cell proliferation c-Myc and C/EBPβ may serve as regulators of polyamine metabolism genes (SMOX, AZIN1, MTAP, SRM, ODC1, AMD1, and AGMAT as they are overexpressed in tumors, have binding site according to ENCODE ChIP-Seq data, and demonstrate strong coexpression with their targets. Thus, increased polyamine metabolism in CRC could be driven by c-Myc and C/EBPβ rather than ETBF infection.

  11. Study of C-MYC amplification and expression in Iranian gastric cancer samples using CISH and IHC methods.

    Science.gov (United States)

    Khaleghian, Malihea; Jahanzad, Issa; Shakoori, Abbas; Ardalan, Farid Azmoudeh; Azimi, Cyrus

    2015-01-01

    Gastric cancer is the fourth most frequent malignancy and the second cause of cancer-related mortality worldwide. It has been suggested that in gastric carcinogenesis, the C-MYC gene has an important function. The objective of this study is to establish the preference of Chromogenic in situ hybridization (CISH) and Immunohistochemistry (IHC) in the diagnosis and prognosis of gastric cancer. Samples comprised of 50 randomly selected patients of whom 40 were male and 10 female. To evaluate the MYC copy number and its protein expression, CISH and IHC analyses were performed for 50 gastric adenocarcinomas, in Iran. The location of the tumor in 64% of the patients was the fundus, and in 72% of patients, the tumors were of a diffuse type; 22 samples showed no amplification, and 28 samples were with amplification. MYC immunoreactivity was observed in 13 samples. Twelve samples showed both MYC amplification and MYC immunoreactivity. In addition, among the 28 CISH+ samples, 12 samples had positive signals for IHC and 16 samples had negative signals for IHC. A majority of the IHC-negative patients had no amplification, but only one patient with IHC positive had no amplification. Our conclusion was that for the management and treatment of gastric cancer, and for special attention of clinicians, for prognosis and tumor progression, the CISH was a better and more feasible test than IHC, in regard to the sensitivity and specificity.

  12. Study of C-MYC amplification and expression in Iranian gastric cancer samples using CISH and IHC methods

    Directory of Open Access Journals (Sweden)

    Malihea Khaleghian

    2015-01-01

    Full Text Available Background: Gastric cancer is the fourth most frequent malignancy and the second cause of cancer-related mortality worldwide. It has been suggested that in gastric carcinogenesis, the C-MYC gene has an important function. The objective of this study is to establish the preference of Chromogenic in situ hybridization (CISH and Immunohistochemistry (IHC in the diagnosis and prognosis of gastric cancer. Materials and Methods: Samples comprised of 50 randomly selected patients of whom 40 were male and 10 female. To evaluate the MYC copy number and its protein expression, CISH and IHC analyses were performed for 50 gastric adenocarcinomas, in Iran. Results: The location of the tumor in 64% of the patients was the fundus, and in 72% of patients, the tumors were of a diffuse type; 22 samples showed no amplification, and 28 samples were with amplification. MYC immunoreactivity was observed in 13 samples. Twelve samples showed both MYC amplification and MYC immunoreactivity. In addition, among the 28 CISH+ samples, 12 samples had positive signals for IHC and 16 samples had negative signals for IHC. A majority of the IHC-negative patients had no amplification, but only one patient with IHC positive had no amplification. Conclusion: Our conclusion was that for the management and treatment of gastric cancer, and for special attention of clinicians, for prognosis and tumor progression, the CISH was a better and more feasible test than IHC, in regard to the sensitivity and specificity.

  13. The adaptive immune system promotes initiation of prostate carcinogenesis in a human c-Myc transgenic mouse model.

    Science.gov (United States)

    Melis, Monique H M; Nevedomskaya, Ekaterina; van Burgsteden, Johan; Cioni, Bianca; van Zeeburg, Hester J T; Song, Ji-Ying; Zevenhoven, John; Hawinkels, Lukas J A C; de Visser, Karin E; Bergman, Andries M

    2017-11-07

    Increasing evidence from epidemiological and pathological studies suggests a role of the immune system in the initiation and progression of multiple cancers, including prostate cancer. Reports on the contribution of the adaptive immune system are contradictive, since both suppression and acceleration of disease development have been reported. This study addresses the functional role of lymphocytes in prostate cancer development using a genetically engineered mouse model (GEMM) of human c-Myc driven prostate cancer (Hi-Myc mice) combined with B and T cell deficiency (RAG1 -/- mice). From a pre-cancerous stage on, Hi-Myc mice showed higher accumulation of immune cells in their prostates then wild-type mice, of which macrophages were the most abundant. The onset of invasive adenocarcinoma was delayed in Hi-MycRAG1 -/- compared to Hi-Myc mice and associated with decreased infiltration of leukocytes into the prostate. In addition, lower levels of the cytokines CXCL2, CCL5 and TGF-β1 were detected in Hi-MycRAG1 -/- compared to Hi-Myc mouse prostates. These results from a GEMM of prostate cancer provide new insights into the promoting role of the adaptive immune system in prostate cancer development. Our findings indicate that the endogenous adaptive immune system does not protect against de novo prostate carcinogenesis in Hi-Myc transgenic mice, but rather accelerates the formation of invasive adenocarcinomas. This may have implications for the development of novel treatment strategies.

  14. Comparison of catching efficiency of two Indonesian traditional traps, Ayunan and Tamba

    Directory of Open Access Journals (Sweden)

    Ahmadi

    2014-08-01

    Full Text Available The catching efficiency of traditional traps: Ayunan and Tamba were tested in Sungai Batang River, South Kalimantan of Indonesia. Trials consisted of 320-trap hauls/type using 1-day submersion time of 24 hr. The baited traps sampling accounted for 82 specimens assigned to 5 species of 5 families. There was a large variability in number of catch between prawns and fish species collected (T=2.318, P<0.05. The prawns catch was represented by only the species Macrobrachium rossenbergii with total of 53 and 1,015 g weight. The prawns weight of Tamba was significantly higher than that of Ayunan (T=3.453, P<0.01.The fish catch composed of Mystus gulio 79%, Osteochilus hasselti 10%, Hypostomus plecostomus 7%, and Macrognathus aculeatus 3%, with total weight ranged from 35 to 560 g. A clear difference was found in catching efficiency. Comparative fishing trials showed that Tamba collected specimens were 1.8 times higher than Ayunan (T=2.223, P<0.05. Catch per unit effort for Tamba ranged from 58.13 to 80.00, and for Ayunan ranged from 5.31 to 7.19. The gear modifications and various treatments (e.g. bait odor, light are necessary to be taken to increase their relative catching efficiency.

  15. Influence of tow duration on catch performance of trawl survey in the Mediterranean Sea.

    Directory of Open Access Journals (Sweden)

    Antonello Sala

    Full Text Available The aim of this study was to assess the effect of tow duration on catch per unit of swept area (CPUE, trawl catch performance, and the proportion of the species caught in a trawl survey. Longer tows are expected to have a greater probability of catching species. An average of 26 species were caught in the first 30 minutes, whereas only about one additional species was caught in the next 30 minutes in longer tows. The shorter tows involved a decrement in catch weight for 11 of the 12 target species sampled, demonstrating that tow duration did affect catch per unit of swept area CPUE. The shorter tows were associated with a significant reduction of the overall CPUE in terms of weight of the main target species and of the total catch (circa 60%. The same strong reduction of around 70% was found in particular for European hake (Merluccius merluccius and surmullet (Mullus spp and 50% for Nephrops (Nephrops norvegicus. The shorter tows were less efficient in catching large-sized hake, surmullet, Nephrops, Atlantic horse mackerel (Trachurus trachurus, and poor cod (Trisopterus minutus, even though the difference was significant only for Nephrops. Regardless of the p-value statistic, these findings suggest that the continuity of survey time series would be severely impaired by changing tow duration. Further work is required to explore a way to reduce tow duration without reducing CPUE.

  16. Innovation, catch-up, and leadership in science-based industries

    OpenAIRE

    Isabel Almudi; Francisco Fatas-Villafranca; Luis R. Izquierdo

    2012-01-01

    In this article, we seek to shed new light on the sources of industrial leadership and catch-up in science-based industries. We propose an evolutionary model that incorporates scientists' training and migration, endogenous R&D decisions, and the possibility of funding capital accumulation through debt. The analysis of the model allows us to characterize a robust pattern of industrial catch-up. Likewise, the sensitivity analysis shows which parameters act as pro-catch-up factors or slow down t...

  17. Catching the light the entwined history of light and mind

    CERN Document Server

    Zajonc, Arthur

    1995-01-01

    In 1910, the surgeons Moreau and LePrince wrote about their successful operation on an eight-year-old boy who had been blind since birth because of cataracts. When the boy's eyes were healed they removed the bandages and, waving a hand in front of the child's physically perfect eyes, asked him what he saw. "I don't know," was his only reply. What he saw was only a varying brightness in front of him. However, when allowed to touch the hand as it began to move, he cried out in a voice of triumph, "It's moving!" He could feel it move, but he still needed laboriously to learn to see it move. Light and eyes were not enough to grant him sight. How, then, do we see? What's the difference between seeing and perception? What is light? From ancient times to the present, from philosophers to quantum physicists, nothing has so perplexed, so fascinated, so captivated the mind as the elusive definition of light. In Catching the Light, Arthur Zajonc takes us on an epic journey into history, tracing how humans have endeavore...

  18. What triggers catch-up saccades during visual tracking?

    Science.gov (United States)

    de Brouwer, Sophie; Yuksel, Demet; Blohm, Gunnar; Missal, Marcus; Lefèvre, Philippe

    2002-03-01

    When tracking moving visual stimuli, primates orient their visual axis by combining two kinds of eye movements, smooth pursuit and saccades, that have very different dynamics. Yet, the mechanisms that govern the decision to switch from one type of eye movement to the other are still poorly understood, even though they could bring a significant contribution to the understanding of how the CNS combines different kinds of control strategies to achieve a common motor and sensory goal. In this study, we investigated the oculomotor responses to a large range of different combinations of position error and velocity error during visual tracking of moving stimuli in humans. We found that the oculomotor system uses a prediction of the time at which the eye trajectory will cross the target, defined as the "eye crossing time" (T(XE)). The eye crossing time, which depends on both position error and velocity error, is the criterion used to switch between smooth and saccadic pursuit, i.e., to trigger catch-up saccades. On average, for T(XE) between 40 and 180 ms, no saccade is triggered and target tracking remains purely smooth. Conversely, when T(XE) becomes smaller than 40 ms or larger than 180 ms, a saccade is triggered after a short latency (around 125 ms).

  19. Mortality of Palmetto bass following catch-and-release angling

    Science.gov (United States)

    Petersen, M.J.; Bettoli, Phillip William

    2013-01-01

    Palmetto bass (Striped Bass Morone saxatilis x White Bass M. chrysops) have been stocked into reservoirs in the southeastern USA since the late 1960s and have gained widespread acceptance as a sport fish. These fisheries are growing in popularity and catch-and-release (CR) fishing is commonplace; however, there is a dearth of information on CR mortality of palmetto bass. We experimentally angled palmetto bass (n = 56; >373-mm TL) in a Tennessee reservoir using traditional angling gear in water temperatures ranging from 13 °C to 32 °C. Ultrasonic transmitters equipped with floats were externally attached to fish, which were released immediately and tracked multiple times within 10 d of release. Mortality was negligible (3.6%) in fall and spring at cool water temperatures but was high (39.3%) in summer when water temperatures exceeded 26 °C. The best logistic regression model based on Akaike's information criterion for small sample sizes scores relied on water temperature alone to predict CR mortality of palmetto bass; there was little support for other models that included all possible combinations of the six other predictor variables we tested. Palmetto bass in our study experienced lower CR mortality than Striped Bass in other systems, but CR mortality rates for palmetto bass that approach or exceed 40% during summer are still problematic if the goal is to maintain fishing quality.

  20. Optimal Passive Dynamics for Physical Interaction: Catching a Mass

    Directory of Open Access Journals (Sweden)

    Kevin Kemper

    2013-05-01

    Full Text Available For manipulation tasks in uncertain environments, intentionally designed series impedance in mechanical systems can provide significant benefits that cannot be achieved in software. Traditionally, the design of actuated systems revolves around sizing torques, speeds, and control strategies without considering the system’s passive dynamics. However, the passive dynamics of the mechanical system, including inertia, stiffness, and damping along with other parameters such as torque and stroke limits often impose performance limitations that cannot be overcome with software control. In this paper, we develop relationships between an actuator’s passive dynamics and the resulting performance for the purpose of better understanding how to tune the passive dynamics for catching an unexpected object. We use a mathematically optimal controller subject to force limitations to stop the incoming object without breaking contact and bouncing. The use of an optimal controller is important so that our results directly reflect the physical system’s performance. We analytically calculate the maximum velocity that can be caught by a realistic actuator with limitations such as force and stroke limits. The results show that in order to maximize the velocity of an object that can be caught without exceeding the actuator’s torque and stroke limits, a soft spring along with a strong damper will be desired.

  1. Environmental DNA from seawater samples correlate with trawl catches of Subarctic, deepwater fishes

    DEFF Research Database (Denmark)

    Thomsen, Philip Francis; Møller, Peter Rask; Sigsgaard, Eva Egelyng

    2016-01-01

    such as bottom trawling, and on official reporting of global catches, which can be unreliable. Thus, there is need for alternative and non-invasive techniques for qualitative and quantitative oceanic fish surveys. Here we report environmental DNA (eDNA) metabarcoding of seawater samples from continental slope...... depths in Southwest Greenland. We collected seawater samples at depths of 188-918 m and compared seawater eDNA to catch data from trawling. We used Illumina sequencing of PCR products to demonstrate that eDNA reads show equivalence to fishing catch data obtained from trawling. Twenty-six families were...... found with both trawling and eDNA, while three families were found only with eDNA and two families were found only with trawling. Key commercial fish species for Greenland were the most abundant species in both eDNA reads and biomass catch, and interpolation of eDNA abundances between sampling sites...

  2. The effect of depth variation on size and catch rate of green tiger ...

    African Journals Online (AJOL)

    moslem

    2013-05-15

    May 15, 2013 ... Analysis of catch. Data analyses were done by Statistical Package for the Social .... important factor affecting spatial distri-bution of juvenile. Jinga shrimp ... resources of Kuwait, Eastern Saudi Arabia, Bahrain, Qatar and the.

  3. The effect of catch crop species on selenium availability for succeeding crops

    DEFF Research Database (Denmark)

    Stavridou, Eleftheria; Young, Scott D.; Thorup-Kristensen, Kristian

    2012-01-01

    2007–10 investigated the ability of catch crops (Italian ryegrass, fodder radish and hairy vetch) under different fertiliser regimes to reduce soil Se content in the autumn and to increase its availability in spring to the succeeding crop. Results and Conclusions The catch crops (Italian ryegrass...... and fodder radish) increased water-extractable Se content in the 0.25–0.75msoil layer in only one of the experiments. Selenium uptake by the catch crops varied between 65 and 3263 mg ha−1, depending on species, year and fertilisation treatment; this corresponded to 0.1–3.0% of the water-extractable soil Se......Background and Aims Selenium (Se) is an essential nutrient for humans and animals. In order to ensure an optimal concentration of Se in crops, Se fertilisers are applied. Catch crops may be an alternative way to increase Se concentrations in vegetables. Methods Three experiments in Denmark between...

  4. Trends in marine fish catches at Pattani Fishery Port (1999-2003

    Directory of Open Access Journals (Sweden)

    Wanchamai Karntanut

    2006-07-01

    Full Text Available This study aims to develop statistical models for forecasting the quantity of the various types of marine fish landed at Pattani Fishery Port, allowing for trend and seasonality, using official data during 1999-2003. The data comprise daily and monthly totals by weight for eight types of fish (mackerel, other food fish, squid, scads, trash fish, shrimp, lobster and crab. The statistical methods are one-way analysis of variance, multiple linear regression and time series forecasting using trend and seasonal models. It is found that mackerel, other food fish and squid catches tend to decrease, whereas the catches of scads tend to increase, and trash fish catches have no detectable trend up or down. Shrimp and lobster tend to decrease exponentially, and the trend of crab catch is constant. This study raises questions about the ecological and economic sustainability of the current fisheries policy in Thailand.

  5. 77 FR 28924 - Requested Administrative Waiver of the Coastwise Trade Laws: Vessel VAN'S CATCH TWO; Invitation...

    Science.gov (United States)

    2012-05-16

    ... entered into this docket is available on the World Wide Web at http://www.regulations.gov . FOR FURTHER...'S CATCH TWO is: Intended Commercial Use of Vessel: ``Sport Fishing Charter.'' Geographic Region...

  6. Technology transfer and catch-up; Lessons from the commercial aircraft industry

    NARCIS (Netherlands)

    Steenhuis, H.J.; de Bruijn, E.J.; Heerkens, Johannes M.G.

    2007-01-01

    This paper analyses the technology development and technology transfer strategies in the aircraft manufacturing industry for four industrially developing countries. It is concluded from four case studies that technology catch-up is extremely difficult due to aircraft technology characteristics.

  7. 76 FR 7155 - Atlantic Highly Migratory Species; Announcement of Billfish and Swordfish Catch Card Pilot...

    Science.gov (United States)

    2011-02-09

    ... Office of Science and Technology, 1315 East West Highway, Silver Spring, MD 20910. FOR FURTHER... landings information. Marinas, tackle shops and other private businesses will be asked to serve as catch...

  8. Maintenance of the classroom health education curricula: results from the CATCH-ON study.

    Science.gov (United States)

    Johnson, Carolyn C; Li, Donglin; Galati, Todd; Pedersen, Sheryl; Smyth, Mary; Parcel, Guy S

    2003-08-01

    Maintenance of the interactive Child and Adolescent Trial for Cardiovascular Health (CATCH) third- to fifth-grade curricula was studied in the 56 original intervention schools and 20 of the original control schools 5 years postintervention in four regions of the United States. Target grade teachers completed a self-administered survey that included questions regarding use of the CATCH materials, training in CATCH or other health education, barriers and perceived support for health education, and amount of health education currently taught. Percentage of teachers who continued to teach CATCH in the classroom was low; however, percentages were significantly higher in former intervention compared with control schools, even though control schools received training and materials following the main field trial. The results of this study can provide useful information for future development of classroom health promotion materials with a higher level of sustainability.

  9. AFSC/FMA/NPRB Alternative Catch Monitoring Table and ccolumn Definitions

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — These data arise from a field study of groundfish catch monitoring in Kodiak, AK trawl fisheries. Two monitoring components were included in the study: 1) at-sea...

  10. 3D view to tumor suppression: Lkb1, polarity and the arrest of oncogenic c-Myc.

    Science.gov (United States)

    Partanen, Johanna I; Nieminen, Anni I; Klefstrom, Juha

    2009-03-01

    Machiavelli wrote, in his famous political treatise Il Principe, about disrupting organization by planting seeds of dissension or by eliminating necessary support elements. Tumor cells do exactly that by disrupting the organized architecture of epithelial cell layers during progression from contained benign tumor to full-blown invasive cancer. However, it is still unclear whether tumor cells primarily break free by activating oncogenes powerful enough to cause chaos or by eliminating tumor suppressor genes guarding the order of the epithelial organization. Studies in Drosophila have exposed genes that encode key regulators of the epithelial apicobasal polarity and which, upon inactivation, cause disorganization of the epithelial layers and promote unscheduled cell proliferation. These polarity regulator/tumor suppressor proteins, which include products of neoplastic tumor suppressor genes (nTSGs), are carefully positioned in polarized epithelial cells to maintain the order of epithelial structures and to impose a restraint on cell proliferation. In this review, we have explored the presence and prevalence of somatic mutations in the human counterparts of Drosophila polarity regulator/tumor suppressor genes across the human cancers. The screen points out LKB1, which is a causal genetic lesion in Peutz-Jeghers cancer syndrome, a gene mutated in certain sporadic cancers and a human homologue of the fly polarity gene par-4. We review the evidence linking Lkb1 protein to polarity regulation in the scope of our recent results suggesting a coupled role for Lkb1 as an architect of organized acinar structures and a suppressor of oncogenic c-Myc. We finally present models to explain how Lkb1-dependent formation of epithelial architecture is coupled to suppression of normal and oncogene-induced proliferation.

  11. Combining telephone surveys and fishing catches self-report: the French sea bass recreational fishery assessment.

    Science.gov (United States)

    Rocklin, Delphine; Levrel, Harold; Drogou, Mickaël; Herfaut, Johanna; Veron, Gérard

    2014-01-01

    Fisheries statistics are known to be underestimated, since they are mainly based on information about commercial fisheries. However, various types of fishing activities exist and evaluating them is necessary for implementing effective management plans. This paper assesses the characteristics and catches of the French European sea bass recreational fishery along the Atlantic coasts, through the combination of large-scale telephone surveys and fishing diaries study. Our results demonstrated that half of the total catches (mainly small fish) were released at sea and that the mean length of a kept sea bass was 46.6 cm. We highlighted different patterns of fishing methods and type of gear used. Catches from boats were greater than from the shore, both in abundance and biomass, considering mean values per fishing trip as well as CPUE. Spearfishers caught the highest biomass of sea bass per fishing trip, but the fishing rod with lure was the most effective type of gear in terms of CPUE. Longlines had the highest CPUE value in abundance but not in biomass: they caught numerous but small sea bass. Handlines were less effective, catching few sea bass in both abundance and biomass. We estimated that the annual total recreational sea bass catches was 3,173 tonnes of which 2,345 tonnes were kept. Since the annual commercial catches landings were evaluated at 5,160 tonnes, recreational landings represent 30% of the total fishing catches on the Atlantic coasts of France. Using fishers' self-reports was a valuable way to obtain new information on data-poor fisheries. Our results underline the importance of evaluating recreational fishing as a part of the total amount of fisheries catches. More studies are critically needed to assess overall fish resources caught in order to develop effective fishery management tools.

  12. Influence of anglers' specializations on catch, harvest, and bycatch of targeted taxa

    Science.gov (United States)

    Pope, Kevin L.; Chizinski, Christopher J.; Wiley, Christopher L.; Martin, Dustin R.

    2016-01-01

    Fishery managers often use catch per unit effort (CPUE) of a given taxon derived from a group of anglers, those that sought said taxon, to evaluate fishery objectives because managers assume CPUE for this group of anglers is most sensitive to changes in fish taxon density. Further, likelihood of harvest may differ for sought and non-sought taxa if taxon sought is a defining characteristic of anglers’ attitude toward harvest. We predicted that taxon-specific catch across parties and reservoirs would be influenced by targeted taxon after controlling for number of anglers in a party and time spent fishing (combine to quantify fishing effort of party); we also predicted similar trends for taxon-specific harvest. We used creel-survey data collected from anglers that varied in taxon targeted, from generalists (targeting “anything” [no primary target taxa, but rather targeting all fishes]) to target specialists (e.g., anglers targeting largemouth bass Micropterus salmoides) in 19 Nebraska reservoirs during 2009–2011 to test our predictions. Taxon-specific catch and harvest were, in general, positively related to fishing effort. More importantly, we observed differences of catch and harvest among anglers grouped by taxon targeted for each of the eight taxa assessed. Anglers targeting a specific taxon had the greatest catch for that taxon and anglers targeting anything typically had the second highest catch for that taxon. In addition, anglers tended to catch more of closely related taxa and of taxa commonly targeted with similar fishing techniques. We encourage managers to consider taxon-specific objectives of target and non-target catch and harvest.

  13. Safety catching device for pipes in missile shielding cylinders of nuclear power plants

    International Nuclear Information System (INIS)

    Hering, S.; Doll, B.

    1976-01-01

    The safety catching device consists of a steel wire passed in U-shape around the pipe to be caught and supported by two anchor ties embedded in the concrete of the missile shielding cylinder. This flexible catching device is to cause the energy released in case of a pipe rupture to be absorbed and no dangerous bending shesses to be transferred to the walls of the missile shielding cylinder. (UWI) [de

  14. Combining telephone surveys and fishing catches self-report: the French sea bass recreational fishery assessment.

    Directory of Open Access Journals (Sweden)

    Delphine Rocklin

    Full Text Available Fisheries statistics are known to be underestimated, since they are mainly based on information about commercial fisheries. However, various types of fishing activities exist and evaluating them is necessary for implementing effective management plans. This paper assesses the characteristics and catches of the French European sea bass recreational fishery along the Atlantic coasts, through the combination of large-scale telephone surveys and fishing diaries study. Our results demonstrated that half of the total catches (mainly small fish were released at sea and that the mean length of a kept sea bass was 46.6 cm. We highlighted different patterns of fishing methods and type of gear used. Catches from boats were greater than from the shore, both in abundance and biomass, considering mean values per fishing trip as well as CPUE. Spearfishers caught the highest biomass of sea bass per fishing trip, but the fishing rod with lure was the most effective type of gear in terms of CPUE. Longlines had the highest CPUE value in abundance but not in biomass: they caught numerous but small sea bass. Handlines were less effective, catching few sea bass in both abundance and biomass. We estimated that the annual total recreational sea bass catches was 3,173 tonnes of which 2,345 tonnes were kept. Since the annual commercial catches landings were evaluated at 5,160 tonnes, recreational landings represent 30% of the total fishing catches on the Atlantic coasts of France. Using fishers' self-reports was a valuable way to obtain new information on data-poor fisheries. Our results underline the importance of evaluating recreational fishing as a part of the total amount of fisheries catches. More studies are critically needed to assess overall fish resources caught in order to develop effective fishery management tools.

  15. Impact of catch shares on diversification of fishers' income and risk.

    Science.gov (United States)

    Holland, Daniel S; Speir, Cameron; Agar, Juan; Crosson, Scott; DePiper, Geret; Kasperski, Stephen; Kitts, Andrew W; Perruso, Larry

    2017-08-29

    Many fishers diversify their income by participating in multiple fisheries, which has been shown to significantly reduce year-to-year variation in income. The ability of fishers to diversify has become increasingly constrained in the last few decades, and catch share programs could further reduce diversification as a result of consolidation. This could increase income variation and thus financial risk. However, catch shares can also offer fishers opportunities to enter or increase participation in catch share fisheries by purchasing or leasing quota. Thus, the net effect on diversification is uncertain. We tested whether diversification and variation in fishing revenues changed after implementation of catch shares for 6,782 vessels in 13 US fisheries that account for 20% of US landings revenue. For each of these fisheries, we tested whether diversification levels, trends, and variation in fishing revenues changed after implementation of catch shares, both for fishers that remained in the catch share fishery and for those that exited but remained active in other fisheries. We found that diversification for both groups was nearly always reduced. However, in most cases, we found no significant change in interannual variation of revenues, and, where changes were significant, variation decreased nearly as often as it increased.

  16. From bonito to anchovy: a reconstruction of Turkey’s marine fisheries catches (1950-2010

    Directory of Open Access Journals (Sweden)

    A. ULMAN

    2013-06-01

    Full Text Available Turkey’s marine fisheries catches were estimated for the 1950-2010 time period using a reconstruction approach, which estimated all fisheries removals, including unreported landings, recreational landings and discards.  We added these estimates to the ‘official’ data, as reported in TURKSTAT, which are also available from the United Nation’s Food and Agriculture Organization (FAO.  The total reconstructed catch for the 1950-2010 time period (inclusive of the reported data is approximately 32 million t, or 74% more than the 18.4 million t of reported data. This added approximately 13.6 million t to the reported data, consisting of 6.9 million t of unreported landings, 2.6 million t of discards, 2.4 million t of recreational catches, and 1.7 million t of subsistence catches.  In 2010, total reported marine landings for Turkey were 445,680 t and the total reconstructed catch was 763,760 t, or 73% more than the reported data.   The main unreported taxon by tonnage was European anchovy (Engraulis encrasicolus due to its sheer high proportion of catch.  The major reasons for underreporting include a general distrust fishers have towards the taxing system combined with inefficient fisheries monitoring and surveillance capabilities.  Accounting for all fisheries components is crucial in understanding the development of fisheries resources, improving management, and reducing threats to the domestic food security of Turkey.

  17. Catching the Light - The Entwined History of Light and Mind

    Science.gov (United States)

    Zajonc, Arthur

    1995-04-01

    In 1910, the surgeons Moreau and LePrince wrote about their successful operation on an eight-year-old boy who had been blind since birth because of cataracts. When the boy's eyes were healed they removed the bandages and, waving a hand in front of the child's physically perfect eyes, asked him what he saw. "I don't know," was his only reply. What he saw was only a varying brightness in front of him. However, when allowed to touch the hand as it began to move, he cried out in a voice of triumph, "It's moving!" He could feel it move, but he still needed laboriously to learn to see it move. Light and eyes were not enough to grant him sight. How, then, do we see? What's the difference between seeing and perception? What is light? From ancient times to the present, from philosophers to quantum physicists, nothing has so perplexed, so fascinated, so captivated the mind as the elusive definition of light. In Catching the Light , Arthur Zajonc takes us on an epic journey into history, tracing how humans have endeavored to understand the phenomenon of light. Blending mythology, religion, science, literature, and painting, Zajonc reveals in poetic detail the human struggle to identify the vital connection between the outer light of nature and the inner light of the human spirit. He explains the curiousness of the Greeks' blue and green "color blindness": Odysseus gazing longingly at the "wine-dark sea"; the use of chloros (green) as the color of honey in Homer's Odessey ; and Euripides' use of the color green to describe the hue of tears and blood. He demonstrates the complexity of perception through the work of Paul Cézanne--the artist standing on the bank of a river, painting the same scene over and over again, the motifs multiplying before his eyes. And Zajonc goes on to show how our quest for an understanding of light, as well as the conclusions we draw, reveals as much about the nature of our own psyche as it does about the nature of light itself. For the ancient

  18. Too late to vaccinate? The incremental benefits and cost-effectiveness of a delayed catch-up program using the 4-valent human papillomavirus vaccine in Norway.

    Science.gov (United States)

    Burger, Emily A; Sy, Stephen; Nygård, Mari; Kristiansen, Ivar S; Kim, Jane J

    2015-01-15

    Human papillomavirus (HPV) vaccines are ideally administered before HPV exposure; therefore, catch-up programs for girls past adolescence have not been readily funded. We evaluated the benefits and cost-effectiveness of a delayed, 1-year female catch-up vaccination program in Norway. We calibrated a dynamic HPV transmission model to Norwegian data and projected the costs and benefits associated with 8 HPV-related conditions while varying the upper vaccination age limit to 20, 22, 24, or 26 years. We explored the impact of vaccine protection in women with prior vaccine-targeted HPV infections, vaccine cost, coverage, and natural- and vaccine-induced immunity. The incremental benefits and cost-effectiveness decreased as the upper age limit for catch-up increased. Assuming a vaccine cost of $150/dose, vaccination up to age 20 years remained below Norway's willingness-to-pay threshold (approximately $83 000/quality-adjusted life year gained); extension to age 22 years was cost-effective at a lower cost per dose ($50-$75). At high levels of vaccine protection in women with prior HPV exposure, vaccinating up to age 26 years was cost-effective. Results were stable with lower coverage. HPV vaccination catch-up programs, 5 years after routine implementation, may be warranted; however, even at low vaccine cost per dose, the cost-effectiveness of vaccinating beyond age 22 years remains uncertain. © The Author 2014. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

  19. Non-Straub type actin from molluscan catch muscle

    Energy Technology Data Exchange (ETDEWEB)

    Shelud' ko, Nikolay S., E-mail: sheludko@stl.ru; Girich, Ulyana V.; Lazarev, Stanislav S.; Vyatchin, Ilya G.

    2016-05-27

    We have developed a method of obtaining natural actin from smooth muscles of the bivalves on the example of the Crenomytilus grayanus catch muscle. The muscles were previously rigorized to prevent a loss of thin filaments during homogenization and washings. Thin filaments were isolated with a low ionic strength solution in the presence of ATP and sodium pyrophosphate. Surface proteins of thin filaments-tropomyosin, troponin, calponin and some minor actin-binding proteins-were dissociated from actin filaments by increasing the ionic strength to 0.6 M KCL. Natural fibrillar actin obtained in that way depolymerizes easily in low ionic strength solutions commonly used for the extraction of Straub-type actin from acetone powder. Purification of natural actin was carried out by the polymerization–depolymerization cycle. The content of inactivated actin remaining in the supernatant is much less than at a similar purification of Straub-type actin. A comparative investigation was performed between the natural mussel actin and the Straub-type rabbit skeletal actin in terms of the key properties of actin: polymerization, activation of Mg-ATPase activity of myosin, and the electron-microscopic structure of actin polymers. -- Highlights: •We developed method of repolymerizable invertebrate smooth muscle actin obtaining. •Our method does not involve use of denaturating agents, which could modify proteins. •Viscosity and polymerization rate of actin, gained that way, is similar to Straub one. •Electron microscopy showed that repolymerized mussel actin is similar to Straub one. •Repolymerized mussel actin has greater ATPase activating capacity, than Straub actin.

  20. Overexpression of c-myc and loss of heterozigosity on 2p, 3p, 5q, 17p and 18q in sporadic colorectal carcinoma Sobreexpresión de c-myc y pérdida de heterozigosidad en 2p, 3p, 5q, 17p y 18q en carcinoma colorrectal esporádico

    Directory of Open Access Journals (Sweden)

    A. Sánchez-Pernaute

    2005-03-01

    Full Text Available Aim: the aim of the present study is to evaluate the prognostic influence of loss of heterozygosity on 2p, 3p, 5q, 17p and 18q, and c-myc overexpression on surgically treated sporadic colorectal carcinoma. Methods: tumor and non-tumor tissue samples from 153 patients were analyzed. Fifty-one percent of patients were male, and mean age in the series was 67 years. Tumors were located in the proximal colon in 37 cases, in the distal bowel in 37, and in the rectum in 79 patients. c-myc overexpression was studied by means of Northern blot analysis, and loss of heterozigosity through microsatellite analysis. Results: c-myc overexpression was detected in 25% of cases, and loss of heterozygosity in at least one of the studied regions in 48%. There was no association between clinical and pathologic features, and genetic alterations. The disease-free interval was significantly shorter for patients with both genetic alterations; the presence of both events was an independent prognostic factor for poor outcome in the multivariate analysis (RR: 4.34, p Objetivo: el objetivo del presente trabajo es evaluar la importancia pronóstica de la pérdida de heterozigosidad en las regiones 2p, 3p, 5q, 17p y 18q y de la sobreexpresión del gen c-myc en el carcinoma colorrectal esporádico, mediante el estudio de la supervivencia libre de enfermedad tras cirugía potencialmente curativa. Métodos: se han analizado muestras tumorales y no tumorales de mucosa colónica de 153 pacientes. El 51% de los pacientes eran varones y la edad media de la serie fue 67 años. Los tumores fueron proximales en 37 casos, distales en 37 y localizados en recto en 79. Se analizó la sobreexpresión del RNA de c-myc por Northern blot, y la presencia de pérdida de heterozigosidad en las diferentes regiones consideradas por análisis de microsatélites. Resultados: se detectó sobreexpresión de c-myc en el 25% de los casos, y pérdida de heterozigosidad en alguna de las regiones estudiadas

  1. Liver tumor formation by a mutant retinoblastoma protein in the transgenic mice is caused by an upregulation of c-Myc target genes

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Bo; Hikosaka, Keisuke; Sultana, Nishat; Sharkar, Mohammad Tofael Kabir [Department of Biochemistry, Hamamatsu University School of Medicine, 1-20-1 Handa-yama, Higashi-ku, Hamamatsu 431-3192 (Japan); Noritake, Hidenao [Department of Biochemistry, Hamamatsu University School of Medicine, 1-20-1 Handa-yama, Higashi-ku, Hamamatsu 431-3192 (Japan); Department of Internal Medicine, Hamamatsu University School of Medicine, 1-20-1 Handa-yama, Higashi-ku, Hamamatsu 431-3192 (Japan); Kimura, Wataru; Wu, Yi-Xin [Department of Biochemistry, Hamamatsu University School of Medicine, 1-20-1 Handa-yama, Higashi-ku, Hamamatsu 431-3192 (Japan); Kobayashi, Yoshimasa [Department of Internal Medicine, Hamamatsu University School of Medicine, 1-20-1 Handa-yama, Higashi-ku, Hamamatsu 431-3192 (Japan); Uezato, Tadayoshi [Department of Biochemistry, Hamamatsu University School of Medicine, 1-20-1 Handa-yama, Higashi-ku, Hamamatsu 431-3192 (Japan); Miura, Naoyuki, E-mail: nmiura@hama-med.ac.jp [Department of Biochemistry, Hamamatsu University School of Medicine, 1-20-1 Handa-yama, Higashi-ku, Hamamatsu 431-3192 (Japan)

    2012-01-06

    Highlights: Black-Right-Pointing-Pointer Fifty percent of the mutant Rb transgenic mice produced liver tumors. Black-Right-Pointing-Pointer In the tumor, Foxm1, Skp2, Bmi1 and AP-1 mRNAs were up-regulated. Black-Right-Pointing-Pointer No increase in expression of the Myc-target genes was observed in the non-tumorous liver. Black-Right-Pointing-Pointer Tumor formation depends on up-regulation of the Myc-target genes. -- Abstract: The retinoblastoma (Rb) tumor suppressor encodes a nuclear phosphoprotein that regulates cellular proliferation, apoptosis and differentiation. In order to adapt itself to these biological functions, Rb is subjected to modification cycle, phosphorylation and dephosphorylation. To directly determine the effect of phosphorylation-resistant Rb on liver development and function, we generated transgenic mice expressing phosphorylation-resistant human mutant Rb (mt-Rb) under the control of the rat hepatocyte nuclear factor-1 gene promoter/enhancer. Expression of mt-Rb in the liver resulted in macroscopic neoplastic nodules (adenomas) with {approx}50% incidence within 15 months old. Interestingly, quantitative reverse transcriptase-PCR analysis showed that c-Myc was up-regulated in the liver of mt-Rb transgenic mice irrespective of having tumor tissues or no tumor. In tumor tissues, several c-Myc target genes, Foxm1, c-Jun, c-Fos, Bmi1 and Skp2, were also up-regulated dramatically. We determined whether mt-Rb activated the Myc promoter in the HTP9 cells and demonstrated that mt-Rb acted as an inhibitor of wild-type Rb-induced repression on the Myc promoter. Our results suggest that continued upregulation of c-Myc target genes promotes the liver tumor formation after about 1 year of age.

  2. SirT1 knockdown potentiates radiation-induced bystander effect through promoting c-Myc activity and thus facilitating ROS accumulation

    Energy Technology Data Exchange (ETDEWEB)

    Xie, Yuexia [Institute of Radiation Medicine, Fudan University, Shanghai (China); Central Laboratory, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai (China); Tu, Wenzhi; Zhang, Jianghong; He, Mingyuan; Ye, Shuang; Dong, Chen [Institute of Radiation Medicine, Fudan University, Shanghai (China); Shao, Chunlin, E-mail: clshao@shmu.edu.cn [Institute of Radiation Medicine, Fudan University, Shanghai (China)

    2015-02-15

    Highlights: • γ-Irradiation induced bystander effects between hepatoma cells and hepatocyte cells. • SirT1 played a protective role in regulating this bystander effect. • SirT1 contributed to the protective effects via elimination the accumulation of ROS. • The activity of c-Myc is critical for maintaining the protective role of SirT1. - Abstract: Radiation-induced bystander effect (RIBE) has important implications for secondary cancer risk assessment during cancer radiotherapy, but the bystander signaling processes, especially under hypoxic condition, are still largely unclear. The present study found that micronuclei (MN) formation could be induced in the non-irradiated HL-7702 hepatocyte cells after being treated with the conditioned medium from irradiated hepatoma HepG2 and SK-Hep-1 cells under either normoxia or hypoxia. This bystander response was dramatically diminished or enhanced when the SirT1 gene of irradiated hepatoma cells was overexpressed or knocked down, respectively, especially under hypoxia. Meanwhile, SirT1 knockdown promoted transcriptional activity for c-Myc and facilitated ROS accumulation. But both of the increased bystander responses and ROS generation due to SirT1-knockdown were almost completely suppressed by c-Myc interference. Moreover, ROS scavenger effectively abolished the RIBE triggered by irradiated hepatoma cells even with SirT1 depletion. These findings provide new insights that SirT1 has a profound role in regulating RIBE where a c-Myc-dependent release of ROS may be involved.

  3. SirT1 knockdown potentiates radiation-induced bystander effect through promoting c-Myc activity and thus facilitating ROS accumulation

    International Nuclear Information System (INIS)

    Xie, Yuexia; Tu, Wenzhi; Zhang, Jianghong; He, Mingyuan; Ye, Shuang; Dong, Chen; Shao, Chunlin

    2015-01-01

    Highlights: • γ-Irradiation induced bystander effects between hepatoma cells and hepatocyte cells. • SirT1 played a protective role in regulating this bystander effect. • SirT1 contributed to the protective effects via elimination the accumulation of ROS. • The activity of c-Myc is critical for maintaining the protective role of SirT1. - Abstract: Radiation-induced bystander effect (RIBE) has important implications for secondary cancer risk assessment during cancer radiotherapy, but the bystander signaling processes, especially under hypoxic condition, are still largely unclear. The present study found that micronuclei (MN) formation could be induced in the non-irradiated HL-7702 hepatocyte cells after being treated with the conditioned medium from irradiated hepatoma HepG2 and SK-Hep-1 cells under either normoxia or hypoxia. This bystander response was dramatically diminished or enhanced when the SirT1 gene of irradiated hepatoma cells was overexpressed or knocked down, respectively, especially under hypoxia. Meanwhile, SirT1 knockdown promoted transcriptional activity for c-Myc and facilitated ROS accumulation. But both of the increased bystander responses and ROS generation due to SirT1-knockdown were almost completely suppressed by c-Myc interference. Moreover, ROS scavenger effectively abolished the RIBE triggered by irradiated hepatoma cells even with SirT1 depletion. These findings provide new insights that SirT1 has a profound role in regulating RIBE where a c-Myc-dependent release of ROS may be involved

  4. Distinct Histopathologic and Molecular Alterations in Inflammatory Bowel Disease-Associated Intestinal Adenocarcinoma: c-MYC Amplification is Common and Associated with Mucinous/Signet Ring Cell Differentiation.

    Science.gov (United States)

    Hartman, Douglas J; Binion, David G; Regueiro, Miguel D; Miller, Caitlyn; Herbst, Cameron; Pai, Reetesh K

    2018-05-17

    Chronic idiopathic inflammatory bowel disease (IBD) is a significant risk factor for the development of intestinal adenocarcinoma. The underlying molecular alterations in IBD-associated intestinal adenocarcinoma remain largely unknown. We compared the clinicopathologic and molecular features of 35 patients with 47 IBD-associated intestinal adenocarcinomas with a consecutive series of 451 patients with sporadic colorectal carcinoma identified at our institution and published data on sporadic colorectal carcinoma. c-MYC amplification was the most frequent molecular alteration identified in 33% of IBD-associated intestinal adenocarcinoma that is a significantly higher frequency than in sporadic colorectal carcinoma (8%) (P = 0.0001). Compared to sporadic colorectal carcinoma, IBD-associated intestinal adenocarcinomas more frequently demonstrated mucinous differentiation (60% vs 25%, P < 0.001) and signet ring cell differentiation (28% vs 4%, P < 0.001). Mucinous and signet ring cell differentiation were significantly associated with the presence of c-MYC amplification (both with P < 0.05). HER2 positivity (11%), KRAS exon 2 or 3 mutation (10%), and IDH1 mutation (7%) were less commonly observed in IBD-associated intestinal adenocarcinoma. There was an association between poor survival and HER2 status with 3 of 4 patients having HER2-positive adenocarcinoma dead of disease at last clinical follow-up; however, no statistically significant survival effect was identified for any of the molecular alterations identified. We demonstrate that IBD-associated intestinal adenocarcinomas have a high frequency of c-MYC amplification that is associated with mucinous and signet ring cell differentiation. Many of the identified molecular alterations have potential therapeutic relevance, including HER2 amplification, IDH1 mutation, and low frequency KRAS mutation.

  5. Increased radiation-induced transformation in C3H/10T1/2 cells after transfer of an exogenous c-myc gene

    International Nuclear Information System (INIS)

    Sorrentino, V.; Drozdoff, V.; Zeitz, L.; Fleissner, E.

    1987-01-01

    C3H/10T 1/2 cells were infected with a retroviral vector expressing a mouse c-myc oncogene and a drug-selection marker. The resulting cells, morphologically indistinguishable from C3H/10T l/1, displayed a greatly enhanced sensitivity to neoplastic transformation by ionizing radiation or by a chemical carcinogen. Constitutive expression of myc therefore appears to synergize with an initial carcinogenic event, providing a function analogous to a subsequent event that apparently is required for the neoplastic transformation of these cells. This cell system should prove useful in exploring early stages in radiation-induced transformation

  6. Angiotensin II reduces cardiac AdipoR1 expression through AT1 receptor/ROS/ERK1/2/c-Myc pathway.

    Directory of Open Access Journals (Sweden)

    Li Li

    Full Text Available Adiponectin, an abundant adipose tissue-derived protein, exerts protective effect against cardiovascular disease. Adiponectin receptors (AdipoR1 and AdipoR2 mediate the beneficial effects of adiponectin on the cardiovascular system. However, the alteration of AdipoRs in cardiac remodeling is not fully elucidated. Here, we investigated the effect of angiotensin II (AngII on cardiac AdipoRs expression and explored the possible molecular mechanism. AngII infusion into rats induced cardiac hypertrophy, reduced AdipoR1 but not AdipoR2 expression, and attenuated the phosphorylations of adenosine monophosphate-activated protein kinase and acetyl coenzyme A carboxylase, and those effects were all reversed by losartan, an AngII type 1 (AT1 receptor blocker. AngII reduced expression of AdipoR1 mRNA and protein in cultured neonatal rat cardiomyocytes, which was abolished by losartan, but not by PD123319, an AT2 receptor antagonist. The antioxidants including reactive oxygen species (ROS scavenger NAC, NADPH oxidase inhibitor apocynin, Nox2 inhibitor peptide gp91 ds-tat, and mitochondrial electron transport chain complex I inhibitor rotenone attenuated AngII-induced production of ROS and phosphorylation of extracellular signal-regulated kinase (ERK 1/2. AngII-reduced AdipoR1 expression was reversed by pretreatment with NAC, apocynin, gp91 ds-tat, rotenone, and an ERK1/2 inhibitor PD98059. Chromatin immunoprecipitation assay demonstrated that AngII provoked the recruitment of c-Myc onto the promoter region of AdipoR1, which was attenuated by PD98059. Moreover, AngII-induced DNA binding activity of c-Myc was inhibited by losartan, NAC, apocynin, gp91 ds-tat, rotenone, and PD98059. c-Myc small interfering RNA abolished the inhibitory effect of AngII on AdipoR1 expression. Our results suggest that AngII inhibits cardiac AdipoR1 expression in vivo and in vitro and AT1 receptor/ROS/ERK1/2/c-Myc pathway is required for the downregulation of AdipoR1 induced by AngII.

  7. Mean temperature of the catch (MTC in the Greek Seas based on landings and survey data

    Directory of Open Access Journals (Sweden)

    Athanassios C. Tsikliras

    2015-04-01

    Full Text Available The mean temperature of the catch (MTC, which is the average inferred temperature preference of the exploited species weighted by their annual catch, is an index that has been used for evaluating the effect of sea warming on marine ecosystems. In the present work, we examined the effect of sea surface temperature on the catch composition of the Greek Seas using the MTC applied on the official catch statistics (landings for the period 1970-2010 (Aegean and Ionian Seas and on experimental bottom trawl survey data for 1997-2014 (southern Aegean Sea. The MTC of the landings for the study period increased from 11.8 οC to 16.2 οC in the Aegean Sea and from 10.0 οC to 14.7 οC in the Ionian Sea. Overall, the rate of MTC increase was 1.01 οC per decade for the Aegean and 1.17 οC per decade for the Ionian Sea and was positively related to sea surface temperature anomalies in both areas. For the survey data, the increase of the MTC of the bottom trawl catch in the southern Aegean Sea was lower (0.51 οC per decade but referred to a shorter time frame and included only demersal species. The change in MTC of official and survey catches indicates that the relative catch proportions of species preferring warmer waters and those preferring colder waters have changed in favour of the former and that this change is linked to sea surface temperature increase, both internally (through the Atlantic Multidecadal Oscillation or externally (warming trend driven.

  8. Relationships among catch, angler catisfaction, and fish assemblage characteristics of an urban small impoundment fishery

    Science.gov (United States)

    Ivasauskas, Tomas J.; Xiong, Wilson N.; Engman, Augustin C.; Fischer, Jesse R.; Kwak, Thomas J.; Rundle, Kirk R.

    2017-01-01

    Urban fisheries provide unique angling opportunities for people from traditionally underrepresented demographics. Lake Raleigh is a 38-ha impoundment located on the North Carolina State University campus in Raleigh. Like many urban fisheries, little is known about angler use and satisfaction or how angling catch rate is related to fish availability in Lake Raleigh. We characterized the recreational fishery and fish assemblage with concurrent creel and boat electrofishing surveys over the course of one year. In total, 245 anglers were interviewed on 68 survey days. On average, anglers spent 1.7 h fishing per trip and caught 0.385 fish h –1. A large proportion of anglers (43.9%) targeted multiple species, whereas 36.5% targeted largemouth bass (Micropterus salmoides), 10.0% targeted panfish (i.e., sunfishes [Lepomis spp.] and crappies [Pomoxis spp.]), and 9.6% targeted catfish (Ameiurus spp. and Ictalurus spp.). Most anglers (69.4%) were satisfied with their experience, and overall satisfaction was unrelated to catch rate. Pulsed-DC boat electrofishing was conducted on 25 dates, and 617 fish were sampled. Angler catch rate was unrelated to electrofishing catch rate, implying that anglers' catch rate was independent of fish density or availability. Our results demonstrate that even minimally managed urban fisheries can provide high angler satisfaction, with limited dedication of management resources. Relationships Among Catch, Angler Satisfaction, and Fish Assemblage Characteristics of an Urban Small Impoundment Fishery (PDF Download Available). Available from: https://www.researchgate.net/publication/316636550_Relationships_Among_Catch_Angler_Satisfaction_and_Fish_Assemblage_Characteristics_of_an_Urban_Small_Impoundment_Fishery [accessed Aug 11, 2017].

  9. Catch rates as indicators of ecosystem health and exploitation status in the shrimp fishery in the South China sea

    DEFF Research Database (Denmark)

    Christensen, Steen; van Thi, Dang

    2008-01-01

    that might reflect seasonality in shrimp recruitment was found, making this resource potentially suitable for a fishery management system based on closed seasons. Further, the data indicate that the major part of the catches are comprised of low value species belonging to the genera Parapenaeopsis; whereas......Based on catch and effort data analyses covering the period 1996-2002, time series of catch rates in the trawl fisheries in the South China Sea along the coasts of Bac Lieu and Ca Mau in South East Vietnam were estimated. The indicators include catch rates for total shrimp catch, five major shrimp...... the most valuable species, i.e. the Penaeus and Metapenaeus catch groups have been significantly depleted during the period investigated. Based on the experiences from the present analysis, recommendations are presented with regard to adjustments of the enumerator data collection programme to fulfil...

  10. The statistical properties of recreational catch rate data for some fish stocks off the northeast U.S. coast

    OpenAIRE

    Terceiro, Mark

    2003-01-01

    Recreational fisheries in the waters off the northeast U.S. target a variety of pelagic and demersal fish species, and catch and effort data sampled from recreational fisheries are a critical component of the information used in resource evaluation and management. Standardized indices of stock abundance developed from recreational fishery catch rates are routinely used in stock assessments. The statistical properties of both simulated and empirical recreational fishery catch-rate data such...

  11. The role of catch crops in the ecological intensification of spring cereals in organic farming under Nordic climate

    DEFF Research Database (Denmark)

    Doltra, Jordi; Olesen, Jørgen E

    2013-01-01

    common practices in organic farming. Measurements of dry matter (DM) and N content of grain cereals at harvest, above-ground biomass in catch crops and green manure crops in autumn and of the green manure crop at the first cutting were performed. The effect of catch crops on grain yield varied...... the nitrate leaching and increasing N retention, but also by improving yields. Management practices in relation to catch crops must be adapted to the specific soil and cropping systems....

  12. Cyclin E/Cdk2, P/CAF, and E1A regulate the transactivation of the c-myc promoter by FOXM1

    International Nuclear Information System (INIS)

    Wierstra, Inken; Alves, Juergen

    2008-01-01

    FOXM1c transactivates the c-myc promoter by binding directly to its TATA-boxes. The present study demonstrates that the transactivation of the c-myc promoter by FOXM1c is enhanced by the key proliferation signal cyclin E/Cdk2, but repressed by P/CAF and the adenoviral oncoprotein E1A. Furthermore, FOXM1c interacts with the coactivator and histone acetyltransferase P/CAF. This study shows that, on the c-myc-P1 TATA-box, FOXM1c does not function simply as normal transcription factor just binding to an unusual site. Moreover, the inhibitory N-terminus of FOXM1c does not inhibit its transrepression domain or its EDA. Others reported that a cyclin/Cdk-binding LXL-motif of the splice variant FoxM1b is required for its interaction with Cdk2, Cdk1, and p27, its phosphorylation by Cdk1 and its activation by Cdc25B. In contrast, we now demonstrate that this LXL-motif is not required for the activation of FOXM1c by cyclin D1/Cdk4, cyclin E/Cdk and cyclin A/Cdk2 or for the repression of FOXM1c by p27

  13. Expression Analysis of p16, c-Myc, and mSin3A in Non-small Cell Lung Cancer by Computer Aided Scoring and Analysis (CASA).

    Science.gov (United States)

    Salmaninejad, Arash; Estiar, Mehrdad Asghari; Gill, Rajbir K; Shih, Joanna H; Hewitt, Stephen; Jeon, Hyo-Sung; Fukuoka, Junya; Shilo, Konstantin; Shakoori, Abbas; Jen, Jin

    2015-01-01

    Immunohistochemical analysis (IHC) of tissue microarray (TMA) slides enables large sets of tissue samples to be analyzed simultaneously on a single slide. However, manual evaluation of small cores on a TMA slide is time consuming and error prone. We describe a computer aided scoring and analysis (CASA) method to allow facile and reliable scoring of IHC staining using TMA containing 300 non-small cell lung cancer (NSCLC) cases. In the two previous published papers utilizing our TMA slides of lung cancer we examined 18 proteins involved in the chromatin machinery. We developed our study using more proteins of the chromatin complex and several transcription factors that facilitate the chromatin machinery. Then, a total of 78 antibodies were evaluated by CASA to derive a normalized intensity value that correlated with the overall staining status of the targeting protein. The intensity values for TMA cores were then examined for association to clinical variables and predictive significance individually and with other factors. RESULTs: Using our TMA, the intensity of several protein pairs were significantly correlated with an increased risk of death in NSCLC. These included c-Myc with p16, mSin3A with p16 and c-Myc with mSinA. Predictive values of these pairs remained significant when evaluated based on standard IHC scores. Our results demonstrate the usefulness of CASA as a valuable tool for systematic assessment of TMA slides to identify potential predictive biomarkers using a large set of primary human tissues.

  14. Absence of DNA damage after 60-Hz electromagnetic field exposure combined with ionizing radiation, hydrogen peroxide, or c-Myc overexpression.

    Science.gov (United States)

    Jin, Yeung Bae; Choi, Seo-Hyun; Lee, Jae Seon; Kim, Jae-Kyung; Lee, Ju-Woon; Hong, Seung-Cheol; Myung, Sung Ho; Lee, Yun-Sil

    2014-03-01

    The principal objective of this study was to assess the DNA damage in a normal cell line system after exposure to 60 Hz of extremely low frequency magnetic field (ELF-MF) and particularly in combination with various external factors, via comet assays. NIH3T3 mouse fibroblast cells, WI-38 human lung fibroblast cells, L132 human lung epithelial cells, and MCF10A human mammary gland epithelial cells were exposed for 4 or 16 h to a 60-Hz, 1 mT uniform magnetic field in the presence or absence of ionizing radiation (IR, 1 Gy), H(2)O(2) (50 μM), or c-Myc oncogenic activation. The results obtained showed no significant differences between the cells exposed to ELF-MF alone and the unexposed cells. Moreover, no synergistic or additive effects were observed after 4 or 16 h of pre-exposure to 1 mT ELF-MF or simultaneous exposure to ELF-MF combined with IR, H(2)O(2), or c-Myc activation.

  15. Temperature effects on pitfall catches of epigeal arthropods: a model and method for bias correction.

    Science.gov (United States)

    Saska, Pavel; van der Werf, Wopke; Hemerik, Lia; Luff, Martin L; Hatten, Timothy D; Honek, Alois; Pocock, Michael

    2013-02-01

    Carabids and other epigeal arthropods make important contributions to biodiversity, food webs and biocontrol of invertebrate pests and weeds. Pitfall trapping is widely used for sampling carabid populations, but this technique yields biased estimates of abundance ('activity-density') because individual activity - which is affected by climatic factors - affects the rate of catch. To date, the impact of temperature on pitfall catches, while suspected to be large, has not been quantified, and no method is available to account for it. This lack of knowledge and the unavailability of a method for bias correction affect the confidence that can be placed on results of ecological field studies based on pitfall data.Here, we develop a simple model for the effect of temperature, assuming a constant proportional change in the rate of catch per °C change in temperature, r , consistent with an exponential Q 10 response to temperature. We fit this model to 38 time series of pitfall catches and accompanying temperature records from the literature, using first differences and other detrending methods to account for seasonality. We use meta-analysis to assess consistency of the estimated parameter r among studies.The mean rate of increase in total catch across data sets was 0·0863 ± 0·0058 per °C of maximum temperature and 0·0497 ± 0·0107 per °C of minimum temperature. Multiple regression analyses of 19 data sets showed that temperature is the key climatic variable affecting total catch. Relationships between temperature and catch were also identified at species level. Correction for temperature bias had substantial effects on seasonal trends of carabid catches. Synthesis and Applications . The effect of temperature on pitfall catches is shown here to be substantial and worthy of consideration when interpreting results of pitfall trapping. The exponential model can be used both for effect estimation and for bias correction of observed data. Correcting for temperature

  16. Pitch catch ultrasonic study on unidirectional CFRP composite laminates using rayleigh wave transducers

    International Nuclear Information System (INIS)

    Park, Je Woong; Yang, In Young; Im, Kwang Hee; Hsu, David K.; Jung, Jong An

    2012-01-01

    The importance of carbon fiber reinforced plastics (CFRP) has been generally recognized, and CFRP composite laminates have become widely used. Thus, a nondestructive technique would be very useful for evaluating CF/epoxy composite laminates. A pitch catch UT signal is more sensitive than is a normal incidence backwall echo of a longitudinal wave in composites. The depth of the sampling volume where the pitch catch UT signal came from is relatively shallow, but the depth can be increased by increasing the separation distance of the transmitting and receiving probes. Moreover, a method is utilized to determine the porosity content of a composite lay up by processing micrograph images of the laminate. The porosity content of a composite structure is critical to the overall strength and performance of the structure. The image processing method developed utilizes software to process micrograph images of the test sample. The results from the image processing method are compared with existing data. Beam profile is characterized in unidirectional CFRP using pitch catch Rayleigh probes. The one sided and two sided pitch catch techniques are utilized to produce C scan images with the aid of an automatic scanner. The pitch catch ultrasonic signal corresponds with the simulated results of unidirectional CFRP composites

  17. A new ball launching system with controlled flight parameters for catching experiments.

    Science.gov (United States)

    d'Avella, A; Cesqui, B; Portone, A; Lacquaniti, F

    2011-03-30

    Systematic investigations of sensorimotor control of interceptive actions in naturalistic conditions, such as catching or hitting a ball moving in three-dimensional space, requires precise control of the projectile flight parameters and of the associated visual stimuli. Such control is challenging when air drag cannot be neglected because the mapping of launch parameters into flight parameters cannot be computed analytically. We designed, calibrated, and experimentally validated an actuated launching apparatus that can control the average spatial position and flight duration of a ball at a given distance from a fixed launch location. The apparatus was constructed by mounting a ball launching machine with adjustable delivery speed on an actuated structure capable of changing the spatial orientation of the launch axis while projecting balls through a hole in a screen hiding the apparatus. The calibration procedure relied on tracking the balls with a motion capture system and on approximating the mapping of launch parameters into flight parameters by means of polynomials functions. Polynomials were also used to estimate the variability of the flight parameters. The coefficients of these polynomials were obtained using the launch and flight parameters of 660 launches with 65 different initial conditions. The relative accuracy and precision of the apparatus were larger than 98% for flight times and larger than 96% for ball heights at a distance of 6m from the screen. Such novel apparatus, by reliably and automatically controlling desired ball flight characteristics without neglecting air drag, allows for a systematic investigation of naturalistic interceptive tasks. Copyright © 2011 Elsevier B.V. All rights reserved.

  18. Project Puffin: How We Brought Puffins Back to Egg Rock.

    Science.gov (United States)

    Kress, Stephen W.; Salmansohn, Pete

    This book describes Project Puffin, a project which aimed to bring puffins that have been threatened with local extinction back to the coast of Maine. The book describes puffins, how they adapted to their ocean environment, how they catch fish, socialize, nest in burrows, and raise their young. (JRH)

  19. Relationships between tuna catch and variable frequency oceanographic conditions

    Directory of Open Access Journals (Sweden)

    F. I. Ormaza-González

    2016-08-01

    analysis of the skipjack catch per unit effort (CPUE on floating aggregating devices (FADs suggests higher CPUE on FADs (around 20 mt set−1 when oceanographic indexes ONI/MEI are below −0.5. Findings of this work suggest that fishing and management of commercial fish must be analyzed under the light of oceanographic conditions.

  20. Test plan for measuring ventilation rates and combustible gas levels in TWRS active catch tanks

    Energy Technology Data Exchange (ETDEWEB)

    NGUYEN, D.M.

    1999-05-20

    The purpose of this test is to provide an initial screening of combustible gas concentrations in catch tanks that currently are operated by Tank Waste Remediation System (TWRS). The data will be used to determine whether or not additional data will be needed for closure of the flammable gas unreviewed safety question for these facilities. This test will involve field measurements of ammonia, organic vapor, and total combustible gas levels in the headspace of the catch tanks. If combustible gas level in a tank exceeds an established threshold, gas samples will be collected in SUMMA canisters for more extensive laboratory analysis. In addition, ventilation rates of some catch tanks will be measured to evaluate removal of flammable gas by air flow through the tanks.

  1. Psychometric Evaluation of the D-Catch, an Instrument to Measure the Accuracy of Nursing Documentation.

    Science.gov (United States)

    D'Agostino, Fabio; Barbaranelli, Claudio; Paans, Wolter; Belsito, Romina; Juarez Vela, Raul; Alvaro, Rosaria; Vellone, Ercole

    2017-07-01

    To evaluate the psychometric properties of the D-Catch instrument. A cross-sectional methodological study. Validity and reliability were estimated with confirmatory factor analysis (CFA) and internal consistency and inter-rater reliability, respectively. A sample of 250 nursing documentations was selected. CFA showed the adequacy of a 1-factor model (chronologically descriptive accuracy) with an outlier item (nursing diagnosis accuracy). Internal consistency and inter-rater reliability were adequate. The D-Catch is a valid and reliable instrument for measuring the accuracy of nursing documentation. Caution is needed when measuring diagnostic accuracy since only one item measures this dimension. The D-Catch can be used as an indicator of the accuracy of nursing documentation and the quality of nursing care. © 2015 NANDA International, Inc.

  2. Sampling, testing and modeling particle size distribution in urban catch basins.

    Science.gov (United States)

    Garofalo, G; Carbone, M; Piro, P

    2014-01-01

    The study analyzed the particle size distribution of particulate matter (PM) retained in two catch basins located, respectively, near a parking lot and a traffic intersection with common high levels of traffic activity. Also, the treatment performance of a filter medium was evaluated by laboratory testing. The experimental treatment results and the field data were then used as inputs to a numerical model which described on a qualitative basis the hydrological response of the two catchments draining into each catch basin, respectively, and the quality of treatment provided by the filter during the measured rainfall. The results show that PM concentrations were on average around 300 mg/L (parking lot site) and 400 mg/L (road site) for the 10 rainfall-runoff events observed. PM with a particle diameter of model showed that a catch basin with a filter unit can remove 30 to 40% of the PM load depending on the storm characteristics.

  3. Environmental DNA from seawater samples correlate with trawl catches of Subarctic, deepwater fishes

    DEFF Research Database (Denmark)

    Thomsen, Philip Francis; Møller, Peter Rask; Sigsgaard, Eva Egelyng

    2016-01-01

    depths in Southwest Greenland. We collected seawater samples at depths of 188-918 m and compared seawater eDNA to catch data from trawling. We used Illumina sequencing of PCR products to demonstrate that eDNA reads show equivalence to fishing catch data obtained from trawling. Twenty-six families were......Remote polar and deepwater fish faunas are under pressure from ongoing climate change and increasing fishing effort. However, these fish communities are difficult to monitor for logistic and financial reasons. Currently, monitoring of marine fishes largely relies on invasive techniques...... such as bottom trawling, and on official reporting of global catches, which can be unreliable. Thus, there is need for alternative and non-invasive techniques for qualitative and quantitative oceanic fish surveys. Here we report environmental DNA (eDNA) metabarcoding of seawater samples from continental slope...

  4. The influence of catch trials on the consolidation of motor memory in force field adaptation tasks

    Directory of Open Access Journals (Sweden)

    Anne eFocke

    2013-07-01

    Full Text Available In computational neuroscience it is generally accepted that human motor memory contains neural representations of the physics of the musculoskeletal system and the objects in the environment. These representations are called internal models. Force field studies, in which subjects have to adapt to dynamic perturbations induced by a robotic manipulandum, are an established tool to analyze the characteristics of such internal models. The aim of the current study was to investigate whether catch trials during force field learning could influence the consolidation of motor memory in more complex tasks. Thereby, the force field was more than double the force field of previous studies (35 Ns/m. Moreover, the arm of the subjects was not supported. A total of forty-six subjects participated in this study and performed center-out movements at a robotic manipulandum in two different force fields. Two control groups learned force field A on day 1 and were retested in the same force field on day 3 (AA. Two test groups additionally learned an interfering force field B (=-A on day 2 (ABA. The difference between the two test and control groups, respectively, was the absence (0% or presence (19% of catch trials, in which the force field was turned off suddenly. The results showed consolidation of force field A on day 3 for both control groups. Test groups showed no consolidation of force field A (19% catch trials and even poorer performance on day 3 (0% catch trials. In conclusion, it can be stated that catch trials seem to have a positive effect on the performance on day 3 but do not trigger a consolidation process as shown in previous studies that used a lower force field viscosity with supported arm. These findings indicate that the results of previous studies in which less complex tasks were analyzed, cannot be fully transferred to more complex tasks. Moreover, the effects of catch trials in these situations are insufficiently understood and further research

  5. Recognition of lameness and decisions to catch for inspection among sheep farmers and specialists in GB

    Directory of Open Access Journals (Sweden)

    Green LE

    2008-10-01

    Full Text Available Abstract Background Epidemiological studies have used farmer estimates of the prevalence of lameness in their flocks. This assumes that farmers can identify lame sheep. Eight movie clips of sheep with locomotion from sound to moderately lame were used to investigate the ability of farmers and sheep specialists to recognise lame sheep. Each participant was asked to complete a form and indicate, for each movie clip, whether they thought the sheep was lame and whether they would catch it if it was the only lame sheep or if 2 – 5, 6 – 10 or > 10 sheep were equally lame. The farmers' responses were compared with their estimates of flock lameness prevalence and the interval between observing a lame sheep and catching it. Results 178 farmers and 54 sheep specialists participated. Participants could identify even mildly lame sheep but made a separate decision on whether to catch them. This decision was dependent on the severity of lameness and the number of sheep lame in a group. Those who said they would catch the first lame sheep in a group were significantly more likely to catch mildly lame sheep (farmer-reported median prevalence of lameness 5% (IQR: 2%–6%. In contrast, farmers who waited for several sheep to be lame indicated that they would only catch more severely lame sheep (farmer reported median flock lameness 11% (IQR: 9%–15%. Approximately 15% of farmers did not catch individual lame sheep (farmer reported median flock lameness 15% (IQR: 10%–15%. The flock prevalence of lameness increased as time to treatment increased and time to treatment was positively correlated with only catching more severely lame sheep. Conclusion If movie-clips are similar to the flock situation, farmers and specialists can recognise even mildly lame sheep but vary in their management from prompt treatment of the first lame sheep in a group to no individual sheep treatments. The former practices would be appropriate to minimise transmission of footrot, a

  6. Reconstructed Marine Fisheries Catches at a Remote Island Group: Pitcairn Islands (1950–2014

    Directory of Open Access Journals (Sweden)

    Amy R. Coghlan

    2017-09-01

    Full Text Available The remote Pitcairn Island Group in the South Pacific was designated one of the world's largest marine reserves in 2016, encompassing some of the few remaining near-pristine areas within EEZ boundaries. Pitcairn's domestic fisheries are small-scale, and consist mainly of subsistence (non-commercial and limited artisanal (commercial catches. There is no locally-based industrial (large-scale commercial fishery and the level of foreign industrial activity in recent times has been minimal, due in part to the low biomass of commercially valuable species, along with economic constraints of the EEZ's geographic isolation. Using a catch reconstruction method we estimated the total domestic marine catches for the Pitcairn Islands from 1950 to 2014. We show that overall the Pitcairn Islands' small-scale fisheries catches were almost 2.5 times higher than the data reported by the Food and Agriculture Organization (FAO of the United Nations on behalf of the Pitcairn Islands, however, this primarily reflects discrepancies prior to the 1980s. Overall, catches for the subsistence and artisanal sectors started with around 12 t·year−1 in 1950, but declined to 4 t·year−1 by 2014. Domestic reconstructed subsistence catch levels were entirely driven by changes in the human population on the island, with reconstructed artisanal catches only occurring in recent years (2000 onwards. Industrial fishing is entirely executed by foreign vessels, this catch is considerably variable throughout the years and ceases entirely in 2006. The implementation of one of the world's largest marine reserves surrounding the offshore waters of Pitcairn Island has been specifically designed not to affect the rates of subsistence and artisanal fishing conducted by the resident population. Although there is no industrial fishing in the Pitcairn EEZ at present, climate change is predicted to influence the routes of migrating commercially-targeted species, potentially altering fishing

  7. Efficacy of Electrocuting Devices to Catch Tsetse Flies (Glossinidae and Other Diptera.

    Directory of Open Access Journals (Sweden)

    Glyn A Vale

    2015-10-01

    Full Text Available The behaviour of insect vectors has an important bearing on the epidemiology of the diseases they transmit, and on the opportunities for vector control. Two sorts of electrocuting device have been particularly useful for studying the behaviour of tsetse flies (Glossina spp, the vectors of the trypanosomes that cause sleeping sickness in humans and nagana in livestock. Such devices consist of grids on netting (E-net to catch tsetse in flight, or on cloth (E-cloth to catch alighting flies. Catches are most meaningful when the devices catch as many as possible of the flies potentially available to them, and when the proportion caught is known. There have been conflicting indications for the catching efficiency, depending on whether the assessments were made by the naked eye or assisted by video recordings.Using grids of 0.5m2 in Zimbabwe, we developed catch methods of studying the efficiency of E-nets and E-cloth for tsetse, using improved transformers to supply the grids with electrical pulses of ~40kV. At energies per pulse of 35-215mJ, the efficiency was enhanced by reducing the pulse interval from 3200 to 1ms. Efficiency was low at 35mJ per pulse, but there seemed no benefit of increasing the energy beyond 70mJ. Catches at E-nets declined when the fine netting normally used became either coarser or much finer, and increased when the grid frame was moved from 2.5cm to 27.5cm from the grid. Data for muscoids and tabanids were roughly comparable to those for tsetse.The catch method of studying efficiency is useful for supplementing and extending video methods. Specifications are suggested for E-nets and E-cloth that are ~95% efficient and suitable for estimating the absolute numbers of available flies. Grids that are less efficient, but more economical, are recommended for studies of relative numbers available to various baits.

  8. Competing probabilistic models for catch-effort relationships in wildlife censuses

    Energy Technology Data Exchange (ETDEWEB)

    Skalski, J.R.; Robson, D.S.; Matsuzaki, C.L.

    1983-01-01

    Two probabilistic models are presented for describing the chance that an animal is captured during a wildlife census, as a function of trapping effort. The models in turn are used to propose relationships between sampling intensity and catch-per-unit-effort (C.P.U.E.) that were field tested on small mammal populations. Capture data suggests a model of diminshing C.P.U.E. with increasing levels of trapping intensity. The catch-effort model is used to illustrate optimization procedures in the design of mark-recapture experiments for censusing wild populations. 14 references, 2 tables.

  9. Nitrogen dynamics following grain legumes and subsequent catch crops and the effects on succeeding cereal crops

    DEFF Research Database (Denmark)

    Hauggaard-Nielsen, Henrik; Mundus, Simon; Jensen, Erik Steen

    2009-01-01

    balances. A 2½-year lysimeter experiment was carried out on a temperate sandy loam soil. Crops were not fertilized in the experimental period and the natural 15N abundance technique was used to determine grain legume N2 fixation. Faba bean total aboveground DM production was significantly higher (1,300 g m...... on the subsequent spring wheat or winter triticale DM production. Nitrate leaching following grain legumes was significantly reduced with catch crops compared to without catch crops during autumn and winter before sowing subsequent spring wheat. Soil N balances were calculated from monitored N leaching from...

  10. A novel form of the RelA nuclear factor kappaB subunit is induced by and forms a complex with the proto-oncogene c-Myc.

    Science.gov (United States)

    Chapman, Neil R; Webster, Gill A; Gillespie, Peter J; Wilson, Brian J; Crouch, Dorothy H; Perkins, Neil D

    2002-01-01

    Members of both Myc and nuclear factor kappaB (NF-kappaB) families of transcription factors are found overexpressed or inappropriately activated in many forms of human cancer. Furthermore, NF-kappaB can induce c-Myc gene expression, suggesting that the activities of these factors are functionally linked. We have discovered that both c-Myc and v-Myc can induce a previously undescribed, truncated form of the RelA(p65) NF-kappaB subunit, RelA(p37). RelA(p37) encodes the N-terminal DNA binding and dimerization domain of RelA(p65) and would be expected to function as a trans-dominant negative inhibitor of NF-kappaB. Surprisingly, we found that RelA(p37) no longer binds to kappaB elements. This result is explained, however, by the observation that RelA(p37), but not RelA(p65), forms a high-molecular-mass complex with c-Myc. These results demonstrate a previously unknown functional and physical interaction between RelA and c-Myc with many significant implications for our understanding of the role that both proteins play in the molecular events underlying tumourigenesis. PMID:12027803

  11. Epithelial-to-mesenchymal transition and c-myc expression are the determinants of cetuximab-induced enhancement of squamous cell carcinoma radioresponse

    International Nuclear Information System (INIS)

    Skvortsova, Ira; Skvortsov, Sergej; Raju, Uma; Stasyk, Taras; Riesterer, Oliver; Schottdorf, Eva-Maria; Popper, Bela-Andre; Schiestl, Bernhard; Eichberger, Paul; Debbage, Paul; Neher, Andreas; Bonn, Guenther K.; Huber, Lukas A.; Milas, Luka; Lukas, Peter

    2010-01-01

    Purpose: Radiation therapy cures malignant tumors of the head and neck region more effectively when it is combined with application of the anti-EGFR monoclonal antibody cetuximab. Despite the successes achieved, we still do not know how to select patients who will respond to this combination of anti-EGFR monoclonal antibody and radiation. This study was conducted to elucidate possible mechanisms which cause the combined treatment with cetuximab and irradiation to fail in some cases of squamous cell carcinomas. Methods and materials: Mice bearing FaDu and A431 squamous cell carcinoma xenograft tumors were treated with cetuximab (total dose 3 mg, intraperitoneally), irradiation (10 Gy) or their combination at the same doses. Treatment was applied when tumors reached 8 mm in size. To collect samples for further protein analysis (two-dimensional differential gel electrophoresis (2-D DIGE), mass spectrometry MALDI-TOF/TOF, Western blot analysis, and ELISA), mice from each group were sacrificed on the 8th day after the first injection of cetuximab. Other mice were subjected to tumor growth delay assay. Results: In FaDu xenografts, treatment with cetuximab alone was nearly as effective as cetuximab combined with ionizing radiation, whereas A431 tumors responded to the combined treatment with significantly enhanced delay in tumor growth. Tumors extracted from the untreated FaDu and A431 xenografts were analysed for protein expression, and 34 proteins that were differently expressed in the two tumor types were identified. The majority of these proteins are closely related to intratumoral angiogenesis, cell adhesion, motility, differentiation, epithelial-to-mesenchymal transition (EMT), c-myc signaling and DNA repair. Conclusions: The failure of cetuximab to enhance radiation response in FaDu xenografts was associated with the initiation of the program of EMT and with c-myc up-regulation in the carcinoma cells. For this reason, c-myc and EMT-related proteins (E

  12. 78 FR 79388 - Western Pacific Pelagic Fisheries; Catch and Effort Limits for the U.S. Participating Territories

    Science.gov (United States)

    2013-12-30

    ... Hawaii longline fisheries: The deep-set fishery that targets bigeye tuna, and the shallow-set fishery... a management framework and process for specifying fishing catch and effort limits and accountability... process for specifying catch or fishing effort limits and accountability measures for pelagic fisheries in...

  13. Meeting the Dietary Goals for School Meals by the Year 2000: The CATCH Eat Smart School Nutrition Program.

    Science.gov (United States)

    Nicklas, Theresa A.; And Others

    1994-01-01

    Provides an overview of the Child and Adolescent Trial for Cardiovascular Health (CATCH) Eat Smart School Nutrition Program, an elementary school health promotion program. The article examines components of the CATCH kitchen visits and intervention materials, including the School Meal Program Guide, Fat and Sodium Criteria, Recipe File Box, Vendor…

  14. An Improved Reconstruction of Total Marine Fisheries Catches for the New Hebrides and the Republic of Vanuatu, 1950–2014

    Directory of Open Access Journals (Sweden)

    Marc Léopold

    2017-10-01

    Full Text Available For many small island nations, fisheries provide residents with both food security and economic stability. However, in order to create effective and sustainable fisheries policies and management that will ensure a growing population can prosper, policy makers need to know what is being fished and how much is fished. Vanuatu, the smallest country in Melanesia, has a declared and claimed Exclusive Economic Zone (EEZ of over 820,000 km2 and fisheries resources play a large part in the food security and economic stability of this country. This reconstruction of the total marine fisheries catch of Vanuatu for 1950–2014 faced major data gaps. It showed that the reconstructed total catches of nearly 1.4 million tonnes (metric tons 40% higher than the 977,997 tonnes reported by the Food and Agriculture Organization (FAO on behalf of Vanuatu for the same period. However, if large-scale industrial catches are excluded, the reconstructed small-scale fisheries catches (~270,000 tonnes were over 200% higher than the 114,862 tonnes of reported catch that were assumed to represent the small-scale sector in FAO data. Subsistence catches made up almost 93% of small-scale catches, followed by artisanal and recreational catches with ~7 and <1%, respectively. By continuously improving the fisheries data of Vanuatu for both the past and the present, policy makers, stakeholders, and fishers can make better decisions that will maintain the benefits of marine fishery resources.

  15. 77 FR 15915 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Comprehensive Annual Catch Limit...

    Science.gov (United States)

    2012-03-16

    ... Amendment, which includes a final environmental impact statement (FEIS), a regulatory flexibility analysis... proposed rule for the Comprehensive ACL Amendment specific to a revised allowable biological catch (ABC... equal to both the OY and the allowable biological catch (ABC). This final rule specifies an ACL for...

  16. Unexpectedly high catch-and-release rates in European marine recreational fisheries: implications for science and management

    DEFF Research Database (Denmark)

    Ferter, Keno; Weltersbach, Marc Simon; Strehlow, Harry Vincent

    2013-01-01

    Unexpectedly high catch-and-release rates in European marine recreational fisheries: implications for science and management. – ICES Journal of Marine Science, 70: .While catch-and-release (C&R) is a well-known practice in several European freshwater recreational fisheries, studies on the magnitu...

  17. Catching Seriously Bad Dudes: US Counter-terrorism strategies

    Czech Academy of Sciences Publication Activity Database

    Bernardyová, Alžběta

    2008-01-01

    Roč. 6, č. 7-8 (2008), s. 34-38 ISSN 1214-1720 R&D Projects: GA ČR GA407/07/1395 Institutional research plan: CEZ:AV0Z70280505 Keywords : terrorism * counter- terrorism * US * 9/11 Subject RIV: AD - Politology ; Political Sciences http://www.socioweb.cz/upl/editorial/download/156_socioweb_7_08.pdf

  18. Influence of the annual flood-pulse on catch per unit effort, condition ...

    African Journals Online (AJOL)

    Influence of the annual flood-pulse on catch per unit effort, condition and reproduction of Clarias gariepinus from the upper Okavango Delta, Botswana. ... therefore, that conservation efforts should be focused on maintaining natural flow patterns in the face of climate change and potential water extraction schemes upstream.

  19. Unemployment and catching up : Europe vis-à-vis the USA

    NARCIS (Netherlands)

    De Groot, Henri L.F.; Van Schaik, Anton B.T.M.

    1997-01-01

    This paper develops a two-region two-sector endogenous growth model with a dual labour market based on efficiency wages. Growth is driven by research done in the (high-tech) tradeables sector. The follower region tends to catch up in terms of labour productivity with the leader region. Differences

  20. Evaluating potential sources of variation in Chironomidae catch rates on sticky traps

    Science.gov (United States)

    Smith, Joshua T.; Muehlbauer, Jeffrey D.; Kennedy, Theodore A.

    2016-01-01

    Sticky traps are a convenient tool for assessing adult aquatic insect population dynamics, but there are many practical questions about how trap sampling artefacts may affect observed results. Utilising study sites on the Colorado River and two smaller streams in northern Arizona, USA, we evaluated whether catch rates and sex ratios of Chironomidae, a ubiquitous aquatic insect, were affected by spraying traps with insecticide, placing traps at different heights above ground, and placing traps at different locations within a terrestrial habitat patch. We also evaluated temporal variation in Chironomidae counts monthly over a 9-month growing season. We found no significant variation in catch rates or sex ratios between traps treated versus untreated with insecticide, nor between traps placed at the upstream or downstream end of a terrestrial habitat patch. Traps placed near ground level did have significantly higher catch rates than traps placed at 1.5 m, although sex ratios were similar across heights. Chironomidae abundance and sex ratios also varied from month-to-month and seemed to be related to climatic conditions. Our results inform future sticky trap studies by demonstrating that trap height, but not insecticide treatment or precise trap placement within a habitat patch, is an important source of variation influencing catch rates.

  1. A hybrid ARIMA and neural network model applied to forecast catch volumes of Selar crumenophthalmus

    Science.gov (United States)

    Aquino, Ronald L.; Alcantara, Nialle Loui Mar T.; Addawe, Rizavel C.

    2017-11-01

    The Selar crumenophthalmus with the English name big-eyed scad fish, locally known as matang-baka, is one of the fishes commonly caught along the waters of La Union, Philippines. The study deals with the forecasting of catch volumes of big-eyed scad fish for commercial consumption. The data used are quarterly caught volumes of big-eyed scad fish from 2002 to first quarter of 2017. This actual data is available from the open stat database published by the Philippine Statistics Authority (PSA)whose task is to collect, compiles, analyzes and publish information concerning different aspects of the Philippine setting. Autoregressive Integrated Moving Average (ARIMA) models, Artificial Neural Network (ANN) model and the Hybrid model consisting of ARIMA and ANN were developed to forecast catch volumes of big-eyed scad fish. Statistical errors such as Mean Absolute Errors (MAE) and Root Mean Square Errors (RMSE) were computed and compared to choose the most suitable model for forecasting the catch volume for the next few quarters. A comparison of the results of each model and corresponding statistical errors reveals that the hybrid model, ARIMA-ANN (2,1,2)(6:3:1), is the most suitable model to forecast the catch volumes of the big-eyed scad fish for the next few quarters.

  2. Retrospective evaluation of factors that influence the implementation of CATCH in southern Illinois schools.

    Science.gov (United States)

    Bice, Matthew R; Brown, Stephen L; Parry, Thomas

    2014-09-01

    Coordinated Approach to Child Health (CATCH) is a school health program implemented in southern Illinois that focuses on physical activity and nutrition and consists of a classroom curriculum, physical education framework, and cafeteria guidelines. Though many schools agreed to implement CATCH, some schools implemented it better than others. This study examined implementation practices of classroom and physical education teachers and cafeteria supervisors. We surveyed 284 school employees at 36 elementary schools located in southern Illinois. Attention focused on organizational readiness, commitment to change, school leadership, implementation barriers, and innovation perceptions concerning degree of implementation of CATCH. Organizational readiness and implementation barriers were significant predictors of degree of implementation for school employees. Additionally, organizational readiness was reported a significant predictor of classroom teacher degree of implementation whereas leadership was a significant predictor of degree of implementation by physical education teachers. Data from this study can be used to enhance implementation of CATCH as well as other school health programs. This study provides educators evidence of why school employees have different implementation practices, evidence of what constructs influence degree of implementation most, and some explanation of school employee degree of implementation. © 2014 Society for Public Health Education.

  3. Assessment of fishing gear and catch rate in Oguta Lake, south ...

    African Journals Online (AJOL)

    This study was carried out in Oguta Lake, Imo State, Nigeria, from January, 2012 to December, 2013 at five stations (Onu Utu, Okposha, Ogbe Hausa, Osemotor and Ede Ngwugwu) to ascertain the percentage abundance and catch rate of gear and craft. The average weight of fish caught per canoe per day ranged between ...

  4. Fish cage culture catches on in Nepal | CRDI - Centre de recherches ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    ... for their children, unlike many rural communities in Nepal. And the role of women in decision-making has been strengthened. Women take part alongside the men in all activities, from cleaning and repairing the fish cages to participating in meetings of farmers'' associations, attending workshops, and marketing the catch.

  5. A trouser bag experiment that quantify by-catch species escaping ...

    African Journals Online (AJOL)

    Sea fishing trials were conducted to quantify by-catch species escaping from a modified shrimp trawl codend and retained in a trouser bag. Modification was by inserting a rectangular aluminum frame excluder device with bar spacing of 20mm at the anterior bunt. Thirty replicate landings showed that; Drepane africana have ...

  6. 76 FR 37285 - Fisheries in the Western Pacific; Mechanism for Specifying Annual Catch Limits and Accountability...

    Science.gov (United States)

    2011-06-27

    ... Limits and Accountability Measures AGENCY: National Marine Fisheries Service (NMFS), National Oceanic and... procedures and timing for specifying annual catch limits (ACLs) and accountability measures (AMs) for western... accountability measures to ensure that the ACL is not exceeded. Restrictions may include, but are not limited to...

  7. 78 FR 48075 - Western Pacific Fisheries; 2013 Annual Catch Limits and Accountability Measures; Correcting...

    Science.gov (United States)

    2013-08-07

    ... DEPARTMENT OF COMMERCE National Oceanic and Atmospheric Administration 50 CFR Part 665 [Docket No. 121107617-3628-03] RIN 0648-XC351 Western Pacific Fisheries; 2013 Annual Catch Limits and Accountability...-Deep 7 bottomfish fishery is not subject to in-season closure or other in- season accountability...

  8. 76 FR 14367 - Fisheries in the Western Pacific; Mechanism for Specifying Annual Catch Limits and Accountability...

    Science.gov (United States)

    2011-03-16

    ... DEPARTMENT OF COMMERCE National Oceanic and Atmospheric Administration 50 CFR Part 665 RIN 0648-AY93 Fisheries in the Western Pacific; Mechanism for Specifying Annual Catch Limits and Accountability... limits (ACLs) and accountability measures (AMs), adopt the ecosystem component species classification...

  9. Root biomass in cereals, catch crops and weeds can be reliably estimated without considering aboveground biomass

    DEFF Research Database (Denmark)

    Hu, Teng; Sørensen, Peter; Wahlström, Ellen Margrethe

    2018-01-01

    and management factors may affect this allometric relationship making such estimates uncertain and biased. Therefore, we aimed to explore how root biomass for typical cereal crops, catch crops and weeds could most reliably be estimated. Published and unpublished data on aboveground and root biomass (corrected...

  10. Small is beautiful: Marine small-scale fisheries catches from the South-West Maluku Regency

    Science.gov (United States)

    Hutubessy, BG; Mosse, JW; Hayward, P.

    2017-10-01

    The fisheries data supplied by fisheries agency have served as the primary tool for regional fisheries statistics. However, it is recognized these data are incomplete and often underestimate actual catches, particularly for small-scale fisheries. There is no widely accepted definition of small-scale fisheries or global data on number of small-scale fishers and their catches. This study reconstructed total marine catches from 1980 to 2015 for South-west Maluku (MBD) regency, by applying an established catch construction approach utilizing all available quantitative and qualitative data, combined with assumption-based estimations and interpolations. As newly established regency since 2009, there is lack of fisheries data available which is needed for fisheries management. Fishers’ knowledge is important information taken from to construct long-term fisheries data. Estimated total fish withdrawal from MBD waters was 86,849.66 tonnes during 1980 - 2015, dominated by pelagic fishes. Consistency of estimated total removal and total landings at MBD regency play important role in small-scale fisheries management and this method of visualizing the history of fishery from poor-data condition might be an optimistic effort.

  11. 50 CFR 300.63 - Catch sharing plan and domestic management measures in Area 2A.

    Science.gov (United States)

    2010-10-01

    ... Federal Register the final rule governing sport fishing in area 2A. Annual management measures may be...) Flexible Inseason Management Provisions for Sport Halibut Fisheries in Area 2A. (1) The Regional... result in exceeding the catch limit for the area. (iii) If any of the sport fishery subareas north of...

  12. Shark fishing effort and catch of the ragged-tooth shark Carcharias ...

    African Journals Online (AJOL)

    An integrated telephone and on-site questionnaire survey was used to estimate total shark fishing effort and specific catch of the ragged-tooth shark Carcharias taurus by coastal club-affiliated shore-anglers, primarily along the east coast of South Africa. Mean total shark fishing effort was estimated to be 37 820 fisherdays ...

  13. Gear selectivity of large-mesh nets and drumlines used to catch ...

    African Journals Online (AJOL)

    Catches of sharks and bycatch in large-mesh nets and baited drumlines used by the Queensland Shark Control Program were examined to determine the efficacy of both gear types and assess fishing strategies that minimise their impacts. There were few significant differences in the size of both sharks and bycatch in the ...

  14. The PS 200 catching trap: A new tool for ultra-low energy antiproton physics

    International Nuclear Information System (INIS)

    Holzscheiter, M.H.; Dyer, P.L.; King, N.S.P.; Lizon, D.C.; Morgan, G.L.; Schauer, M.M.; Schecker, J.A.; Hoibraten, S.; Lewis, R.A.; Otto, T.

    1994-01-01

    Approximately one million antiprotons have been trapped and electron cooled in the PS200 catching trap from a single fast extracted pulse from LEAR. The system is described in detail, different extraction schemes are discussed, and possible applications of this instrument to ultra-low energy atomic and nuclear physics with antiprotons are mentioned

  15. Men are catching up with women in the life expectancy game.

    Science.gov (United States)

    2016-08-01

    One reason men are catching up is because of falling smoking rates. To give an idea of the powerful effect of smoking rates on mortality, 800,000 American deaths from lung cancer have been avoided because of the decline in smoking rates between 1975 and 2000.

  16. Spatial and temporal variation in artisanal catches of dolphinfish Coryphaena hippurus off north-eastern Brazil.

    Science.gov (United States)

    Nóbrega, M F; Kinas, P G; Lessa, R; Ferrandis, E

    2015-02-01

    The sampling of fish from the artisanal fleet operating with surface lines off north-eastern Brazil was carried out between 1998 and 2000. Generalized linear models (GLMs) were used to standardize mean abundance indices using catch and fishing effort data on dolphinfish Coryphaena hippurus and to identify abundance trends in time and space, using 1215 surface line deployments. A standard relative abundance index (catch per unit effort, CPUE) was estimated for the most frequent vessels used in the sets, employing factors and coefficients generated in the GLMs. According to the models, C. hippurus catches are affected by the operating characteristics and power of different fishing vessels. These differences highlight the need for standardization of catch and effort data for artisanal fisheries. The highest mean abundance values for C. hippurus were off the state of Rio Grande do Norte, with an increasing tendency in areas with greater depths and more distant from the coast, reaching maximal values in areas whose depths range from 200 to 500 m. The highest mean abundance values occurred between April and June. The higher estimated abundance of C. hippurus in this period off the state of Rio Grande do Norte and within the 200-500 m depth range may be related to a migration pattern of food sources, as its main prey, the flying fish Hirundichthys affinis, uses floating algae as refuge and to deposit its pelagic eggs. © 2015 The Fisheries Society of the British Isles.

  17. Employment assimilation of immigrants in the Netherlands: Catching up and the irrelevance of education

    NARCIS (Netherlands)

    Zorlu, A.; Hartog, J.

    2008-01-01

    Using two Dutch labour force surveys, employment assimilation of immigrants is examined. We observe marked differences between immigrants by source country. Non-western immigrants never reach parity with native Dutch. Even second generation immigrants never fully catch up. Caribbean immigrants, who

  18. Employment assimilation of immigrants in the Netherlands: catching up and the irrelevance of education

    NARCIS (Netherlands)

    Zorlu, A.; Hartog, J.

    2008-01-01

    Using two Dutch labour force surveys, employment assimilation of immigrants is examined. We observe marked differences between immigrants by source country. Non-western immigrants never reach parity with native Dutch. Even second generation immigrants never fully catch up. Caribbean immigrants, who

  19. IMPACT OF CLIMATE ANOMALY ON CATCH COMPOSITION OF NERITIC TUNA IN SUNDA STRAIT

    Directory of Open Access Journals (Sweden)

    Khairul Amri

    2013-12-01

    Full Text Available Tongkol komo/kawakawa (Euthynnus affinis and tenggiri (Scomberomerus guttatus are commonly caught by mini purseiners operated in Sunda Straits and landed in Labuan, West Java. This species inhabits coastal water and has preference staying in relatively warm water. Oceanography parameters commonly influencing the distribution of Euthynnus affinis are temperature, current, and salinity. The oceanography of Sunda Strait is influenced by water masses coming from the north that mainly originated from the Java Sea and water masses from the south mainly originated from Indian Ocean. The internal oceanography of Sunda Strait is also influenced by upwelling and monsoon as regional climate anomaly (ENSO and Indian Ocean Dipole Mode. This paper describes the influence of Dipole Mode (positive and negative event and ENSO (El- Nino/La-Nina to the catch dynamics of neritic tuna particularly in Sunda Straits waters. The results shown that regional climate anomaly influenced neritic tuna catch and its composition. The catches Euthynnus affinis in phase negative dipole mode or La-Nina were higher and dominated the catch composition of pelagic fishes of Sunda Strait. Similar situation also is showen by Scomberomorus commerson.

  20. 75 FR 55305 - Proposed Information Collection; Comment Request; Social Impacts of the Implementation of a Catch...

    Science.gov (United States)

    2010-09-10

    ... DEPARTMENT OF COMMERCE National Oceanic and Atmospheric Administration Proposed Information Collection; Comment Request; Social Impacts of the Implementation of a Catch Shares Program in the Mid... Department of Commerce, as part of its continuing effort to reduce paperwork and respondent burden, invites...

  1. Library of biphenyl privileged substructures using a safety-catch linker approach

    DEFF Research Database (Denmark)

    Severinsen, Rune; Bourne, Gregory T; Tran, Tran T

    2008-01-01

    A biphenyl privileged structure library containing three attachment points were synthesized using a catechol-based safety-catch linker strategy. The method requires the attachment of a bromo-acid to the linker, followed by a Pd-catalyzed Suzuki cross-coupling reaction. Further derivatization...

  2. Gill net selectivity and catch rates of pelagic fish in tropical coastal ...

    African Journals Online (AJOL)

    STORAGESEVER

    2008-11-05

    Nov 5, 2008 ... fish communities includes a decrease in their abundance, changes in age structure, size composition ... index (phytoplankton primary productivity, total phospho- ... conservation of biodiversity and the maintenance of a ... distribution of the catches and to understand the popu- ... lagoon beach (Figure 1).

  3. Relationship between weekend catch-up sleep and poor performance on attention tasks in Korean adolescents.

    Science.gov (United States)

    Kim, Seog Ju; Lee, Yu Jin; Cho, Seong-Jin; Cho, In-Hee; Lim, Weonjeong; Lim, Wonshin

    2011-09-01

    To investigate the relationship between insufficient sleep and poor attention in Korean adolescents, adjusting for potential confounding factors of depressed mood and habitual snoring. School-based cross-sectional study. Eight high schools in 3 cities in the Republic of Korea. A sample of 2638 urban high school students (42.2% male and 57.8% female; mean [SD] age, 17.3 [0.6] years [age range, 14-19 years]) completed questionnaires and computerized attention tasks during the school term. Weekend catch-up sleep. Self-reported sleep schedules and habits, including sleep duration, bedtime, wake-up time, depressed mood, and habitual snoring. Also measured were numbers of omission and commission errors on computerized attention tasks. The mean (SD) sleep duration on weekdays was 5 hours 42 minutes (1 hour 0 minutes) per day and on weekends was 8 hours 24 minutes (1 hour 36 minutes) per day. The mean (SD) weekend catch-up sleep was 2 hours 42 minutes (1 hour 42 minutes) per day. After adjusting for age, sex, depressed mood (Beck Depression Inventory score, ≥10), habitual snoring, and weekday sleep duration, increased weekend catch-up sleep was significantly associated with more omission and commission errors on sustained attention tasks (P sleep as an indicator of insufficient weekday sleep is associated with poor performance on objective attention tasks. Assessment of catch-up sleep and sleep duration may be useful for physicians to evaluate sleep insufficiency and its adverse effects on attention in adolescents.

  4. Do North Sea cod (Gadus morhua) fisheries maintain high catch rates at low stock size?

    DEFF Research Database (Denmark)

    Rindorf, Anna; Andersen, Bo Sølgaard

    2008-01-01

    This study presents all investigation of the relationship between stock size of North Sea cod (Gadus morhua) and catch rates in seven commercial fishing fleets. The shape of the relationship was estimated using a model allowing both density-dependent changes in catchability and bias...

  5. Foreign Direct Investments in Poland and the Quality Catch-Up of Polish Foreign Trade

    DEFF Research Database (Denmark)

    Nielsen, Jørgen Ulff-Møller

    The concept of unit value is shown to be a useful tool in analysing changes in the price-quality competitiveness of the export of Poland, which, until 1996 has shown signs of a quality "catching-up" for products surviving international competition. However, the quality level of export products is...

  6. Nitrogen release from differently aged Raphanus sativus L. nitrate catch crops during mineralization at autumn temperatures

    DEFF Research Database (Denmark)

    Thomsen, Ingrid Kaag; Elsgaard, Lars; Olesen, Jørgen Eivind

    2016-01-01

    radish (Raphanus sativus, L.) has emerged as a promising nitrate catch crop in cereal cropping, although the course of remineralization of residue N following termination of this frost-sensitive crucifer remains obscured. We incubated radish residues of different age (different planting and harvest dates...

  7. Nitrate Leaching, Yields and Carbon Sequestration after Noninversion Tillage, Catch Crops, and Straw Retention

    DEFF Research Database (Denmark)

    Hansen, Elly Møller; Munkholm, Lars Juhl; Olesen, Jørgen E

    2015-01-01

    retention did not significantly increase yields, nor did it reduce leaching, while fodder radish (Raphanus sativus L.) as a catch crop was capable of reducing nitrate leaching to a low level. Thus, YSL of winter wheat (Triticum aestivum L.) was higher than for spring barley (Hordeum vulgare L.) grown after...

  8. Effects of catch crop type and root depth on nitrogen leaching and yield of spring barley

    DEFF Research Database (Denmark)

    Sapkota, Tek Bahadur; Askegaard, Margrethe; Lægdsmand, Mette

    2012-01-01

    [chicory (Cichorium intybus L.), fodder radish (Raphanus sativus L.) and perennial ryegrass (Lolium perenne L.)] and their effect on soil mineral N (NO3− and NH4+) in different soil layers by using the FASSET model. The simulated results of catch crop biomass and root growth and mineral N in the soil...

  9. Brasil (1955-2005: 25 anos de catching up, 25 anos de falling behind

    Directory of Open Access Journals (Sweden)

    Marcelo Arend

    2012-03-01

    Full Text Available Brazil (1955-2005: 25 years of catching-up, 25 years of falling behind. The present paper discusses the Brazilian industrial development under a neo-schumpeterian perspective in the period after 1955. The hypothesis is that, in the last 50 years, Brazil spent the first 25 years catching up and, next, the following 25 years falling behind. The 1955-1980 period, by means of international funding, allowed catching up with the paradigm in maturation within the fourth technological revolution. However, in this period, it was determined the main debilitating elements for the country's entrance in the new techno-economical paradigm of the fifth technological revolution which emerged in the middle of the 70s. It is in the strategy to internationalize the economy, granting the mutinational companies the key-sectors of the national economy dynamics during the catching up period, the main element of dependence in the journey that conditions the current performance, responsible for technology subordination and keeps the Brazilian economy with low dynamism.

  10. A robust method for generating separate catch time-series for each ...

    African Journals Online (AJOL)

    Here, species-separated data from an observer programme and scientific surveys are used to model the spatio-temporal overlap of the two species, which are then used to predict the catch by species in each commercial trawl. The study presents a method that compensates for both the escapement and codend retention ...

  11. Factors influencing bruises and mortality of broilers during catching, transport, and lairage

    NARCIS (Netherlands)

    Nijdam, E.; Arens, P.; Lambooij, E.; Decuypere, E.; Stegeman, J.A.

    2004-01-01

    A multilevel analysis was performed to identify and quantify risk factors associated with mortality and bruises occurring between catching and slaughter of broiler flocks. The effect of each factor in the final model was expressed as an odds ratio (OR). Data included 1,907 Dutch and German broiler

  12. Gradual catch up and enduring leadership in the global wine industry

    NARCIS (Netherlands)

    Morrison, A.; Rabellotti, Roberta

    The wine industry is an extremely interesting sector from a catch -up point of view because the latecomers in the international market have changed how wine is produced, sold and consumed and, in doing so, they have challenged the positions of incumbents. Until the end of the 1980s, the European

  13. Protein kinase A antagonist inhibits β-catenin nuclear translocation, c-Myc and COX-2 expression and tumor promotion in ApcMin/+ mice

    Directory of Open Access Journals (Sweden)

    Brudvik Kristoffer W

    2011-12-01

    Full Text Available Abstract Background The adenomatous polyposis coli (APC protein is part of the destruction complex controlling proteosomal degradation of β-catenin and limiting its nuclear translocation, which is thought to play a gate-keeping role in colorectal cancer. The destruction complex is inhibited by Wnt-Frz and prostaglandin E2 (PGE2 - PI-3 kinase pathways. Recent reports show that PGE2-induced phosphorylation of β-catenin by protein kinase A (PKA increases nuclear translocation indicating two mechanisms of action of PGE2 on β-catenin homeostasis. Findings Treatment of ApcMin/+ mice that spontaneously develop intestinal adenomas with a PKA antagonist (Rp-8-Br-cAMPS selectively targeting only the latter pathway reduced tumor load, but not the number of adenomas. Immunohistochemical characterization of intestines from treated and control animals revealed that expression of β-catenin, β-catenin nuclear translocation and expression of the β-catenin target genes c-Myc and COX-2 were significantly down-regulated upon Rp-8-Br-cAMPS treatment. Parallel experiments in a human colon cancer cell line (HCT116 revealed that Rp-8-Br-cAMPS blocked PGE2-induced β-catenin phosphorylation and c-Myc upregulation. Conclusion Based on our findings we suggest that PGE2 act through PKA to promote β-catenin nuclear translocation and tumor development in ApcMin/+ mice in vivo, indicating that the direct regulatory effect of PKA on β-catenin nuclear translocation is operative in intestinal cancer.

  14. Genetic dissimilarity between primary colorectal carcinomas and their lymph node metastases: ploidy, p53, bcl-2, and c-myc expression--a pilot study.

    Science.gov (United States)

    Zalata, Khaled Refaat; Elshal, Mohamed Farouk; Foda, Abd AlRahman Mohammad; Shoma, Ashraf

    2015-08-01

    The current paradigm of metastasis proposes that rare cells within primary tumors acquire metastatic capability via sequential mutations, suggesting that metastases are genetically dissimilar from their primary tumors. This study investigated the changes in the level of expression of a well-defined panel of cell proliferation, differentiation, and apoptosis markers between the primary colorectal cancer (CRC) and the corresponding synchronous lymph node (LN) metastasis from the same patients. DNA flow cytometry and immunostaining of p53, bcl-2, and c-myc were carried out on 36 cases of CRC radical resection specimens with their corresponding LN metastases. There was very low probability that the histological patterns of primary tumors and LN metastases are independent (p < 0.001). Metastatic tumors were significantly more diffusely positive for p53 than the primary tumors (p < 0.001). Conversely, primary tumors were significantly more diffusely positive for c-myc than metastatic tumors (p = 0.011). No significant difference was found between the LNs and the primary tumors in bcl-2 positivity (p = 0.538) and DNA aneuploidy (p = 0.35), with a tendency towards negative bcl-2 and less aneuploidy in LN metastases than primary tumors. In conclusion, LN metastatic colorectal carcinomas have a tendency of being less differentiated, with a higher incidence of diffuse p53 staining, lower incidence of bcl-2 staining, and less aneuploidy in comparison to their primary counterparts suggesting a more aggressive biological behavior, which could indicate the necessity for more aggressive adjuvant therapy.

  15. Soluble expression of recomb inant cMyc, Klf4, Oct4, and Sox2 proteins in bacteria and transduction into living cells

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    Guo-Dan Liu

    2017-04-01

    Full Text Available AIM: To develop a new method to produce recombinant reprogramming proteins, cMyc, Klf4, Oct4, and Sox2, in soluble format with low cost for the generation of induced pluripotent stem cells (iPSCs. METHODS: A short polypeptide sequence derived from the HIV trans-activator of transcription protein (TAT and the nucleus localization signal (NLS polypeptide were fused to the N terminus of the reprogramming proteins and they were constructed into pCold-SUMO vector which can extremely improve the solubility of recombinant proteins. Then these vector plasmids were transformed into E. coli BL21 (DE3 Chaperone competent cells for amplification. The solubility of these recombinant proteins was determined by SDS-PAGE and Coomassie brilliant blue staining. The recombinant proteins were purified by Ni-NTA resin and identified by Western blot. The transduction of these proteins into HEK 293T cells were evaluated by immunofluorescence staining. RESULTS: These four reprogramming proteins could be produced in soluble format in pCold-SUMO expression vector system with the assistance of chaperone proteins in bacteria. The proteins were purified successfully with a purity of over 70% with a relative high transduction rate into 293 cells. CONCLUSION: The results in the present study indicate the four important reprogramming proteins, cMyc, Klf4, Oct4, and Sox2, can be produced in soluble format in bacteria with low cost. Our new method thus might be expected to greatly contribute to the future study of iPSCs.

  16. Oridonin induces apoptosis and senescence in colorectal cancer cells by increasing histone hyperacetylation and regulation of p16, p21, p27 and c-myc

    International Nuclear Information System (INIS)

    Gao, Feng-Hou; Liu, Feng; Zhao, Ying-Zheng; Fang, Yong; Chen, Fang-Yuan; Wu, Ying-Li; Hu, Xiao-Hui; Li, Wei; Liu, Hua; Zhang, Yan-Jie; Guo, Zhu-Ying; Xu, Mang-Hua; Wang, Shi-Ting; Jiang, Bin

    2010-01-01

    Oridonin, a tetracycline diterpenoid compound, has the potential antitumor activities. Here, we evaluate the antitumor activity and action mechanisms of oridonin in colorectal cancer. Effects of oridonin on cell proliferation were determined by using a CCK-8 Kit. Cell cycle distribution was determined by flow cytometry. Apoptosis was examined by analyzing subdiploid population and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay. Senescent cells were determined by senescence-associated β-galactosidase activity analysis. Semi-quantitative RT-PCR was used to examine the changes of mRNA of p16, p21, p27 and c-myc. The concomitant changes of protein expression were analyzed with Western blot. Expression of AcH3 and AcH4 were examined by immunofluorescence staining and Western blots. Effects of oridonin on colony formation of SW1116 were examined by Soft Agar assay. The in vivo efficacy of oridonin was detected using a xenograft colorectal cancer model in nude mice. Oridonin induced potent growth inhibition, cell cycle arrest, apoptosis, senescence and colony-forming inhibition in three colorectal cancer cell lines in a dose-dependent manner in vitro. Daily i.p. injection of oridonin (6.25, 12.5 or 25 mg/kg) for 28 days significantly inhibited the growth of SW1116 s.c. xenografts in BABL/C nude mice. With western blot and reverse transcription-PCR, we further showed that the antitumor activities of oridonin correlated with induction of histone (H3 and H4) hyperacetylation, activation of p21, p27 and p16, and suppression of c-myc expression. Oridonin possesses potent in vitro and in vivo anti-colorectal cancer activities that correlated with induction of histone hyperacetylation and regulation of pathways critical for maintaining growth inhibition and cell cycle arrest. Therefore, oridonin may represent a novel therapeutic option in colorectal cancer treatment

  17. Oridonin induces apoptosis and senescence in colorectal cancer cells by increasing histone hyperacetylation and regulation of p16, p21, p27 and c-myc

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    Zhao Ying-Zheng

    2010-11-01

    Full Text Available Abstract Background Oridonin, a tetracycline diterpenoid compound, has the potential antitumor activities. Here, we evaluate the antitumor activity and action mechanisms of oridonin in colorectal cancer. Methods Effects of oridonin on cell proliferation were determined by using a CCK-8 Kit. Cell cycle distribution was determined by flow cytometry. Apoptosis was examined by analyzing subdiploid population and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay. Senescent cells were determined by senescence-associated β-galactosidase activity analysis. Semi-quantitative RT-PCR was used to examine the changes of mRNA of p16, p21, p27 and c-myc. The concomitant changes of protein expression were analyzed with Western blot. Expression of AcH3 and AcH4 were examined by immunofluorescence staining and Western blots. Effects of oridonin on colony formation of SW1116 were examined by Soft Agar assay. The in vivo efficacy of oridonin was detected using a xenograft colorectal cancer model in nude mice. Results Oridonin induced potent growth inhibition, cell cycle arrest, apoptosis, senescence and colony-forming inhibition in three colorectal cancer cell lines in a dose-dependent manner in vitro. Daily i.p. injection of oridonin (6.25, 12.5 or 25 mg/kg for 28 days significantly inhibited the growth of SW1116 s.c. xenografts in BABL/C nude mice. With western blot and reverse transcription-PCR, we further showed that the antitumor activities of oridonin correlated with induction of histone (H3 and H4 hyperacetylation, activation of p21, p27 and p16, and suppression of c-myc expression. Conclusion Oridonin possesses potent in vitro and in vivo anti-colorectal cancer activities that correlated with induction of histone hyperacetylation and regulation of pathways critical for maintaining growth inhibition and cell cycle arrest. Therefore, oridonin may represent a novel therapeutic option in colorectal cancer treatment.

  18. PET/CT imaging of c-Myc transgenic mice identifies the genotoxic N-nitroso-diethylamine as carcinogen in a short-term cancer bioassay.

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    Katja Hueper

    Full Text Available BACKGROUND: More than 100,000 chemicals are in use but have not been tested for their safety. To overcome limitations in the cancer bioassay several alternative testing strategies are explored. The inability to monitor non-invasively onset and progression of disease limits, however, the value of current testing strategies. Here, we report the application of in vivo imaging to a c-Myc transgenic mouse model of liver cancer for the development of a short-term cancer bioassay. METHODOLOGY/PRINCIPAL FINDINGS: μCT and ¹⁸F-FDG μPET were used to detect and quantify tumor lesions after treatment with the genotoxic carcinogen NDEA, the tumor promoting agent BHT or the hepatotoxin paracetamol. Tumor growth was investigated between the ages of 4 to 8.5 months and contrast-enhanced μCT imaging detected liver lesions as well as metastatic spread with high sensitivity and accuracy as confirmed by histopathology. Significant differences in the onset of tumor growth, tumor load and glucose metabolism were observed when the NDEA treatment group was compared with any of the other treatment groups. NDEA treatment of c-Myc transgenic mice significantly accelerated tumor growth and caused metastatic spread of HCC in to lung but this treatment also induced primary lung cancer growth. In contrast, BHT and paracetamol did not promote hepatocarcinogenesis. CONCLUSIONS/SIGNIFICANCE: The present study evidences the accuracy of in vivo imaging in defining tumor growth, tumor load, lesion number and metastatic spread. Consequently, the application of in vivo imaging techniques to transgenic animal models may possibly enable short-term cancer bioassays to significantly improve hazard identification and follow-up examinations of different organs by non-invasive methods.

  19. Disturbance of Bcl-2, Bax, Caspase-3, Ki-67 and C-myc expression in acute and subchronic exposure to benzo(a)pyrene in cervix.

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    Gao, Meili; Li, Yongfei; Ji, Xiaoying; Xue, Xiaochang; Chen, Lan; Feng, Guodong; Zhang, Huqin; Wang, Huichun; Shah, Walayat; Hou, Zhanwu; Kong, Yu

    2016-03-01

    Epidemiological studies have demonstrated that cigarette smoking is an important cofactor or an independent risk factor for the development of cervical cancer. Benzo(a)pyrene (BaP) is one of the most potent tobacco smoke carcinogens in tobacco smoke. BaP induced DNA damage and over expression in p53 cervical tissue of mice as demonstrated in our previous study. Here we present the findings of exposure to BaP on the expression of Bcl-2, C-myc, Ki-67, Caspase-3 and Bax genes in mouse cervix. Acute intraperitoneal administration of BaP (12.5, 25, 50, 100mg/kg body weight) to ICR female mice induced a significant increase in Bcl-2, C-myc, Ki-67 mRNA and protein level till 72h except in Bcl-2 at 24h with 12.5, 25, 50mg/kg as well as at 48h with 12.5mg/kg body weight post treatment. A significant increase was also seen in Caspase-3 and Bax mRNA and protein level with peak level at 24h and gradual decrease till 72h, however, the expression of caspase-3 increased while that of Bax decreased with increasing dose of Bap after 24h. In sub chronic intraperitoneal and oral gavage administration of BaP (2.5, 5, 10mg/kg body weight), similar significant increase was observed for all the examined genes as compared to the control and vehicle groups, however the expression of Bax decreased in a dose dependent manner. The findings of this study will help in further understanding the molecular mechanism of BaP induced carcinogenesis of cervical cancer. Copyright © 2015 Elsevier GmbH. All rights reserved.

  20. On rational usage of catches during the trap fishery in the Barents Sea

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    Sokolov K. M.

    2017-06-01

    Full Text Available The aim of the work is the search for resources to increase the fullness and complexity of using commercial catches of the red king crab and snow crab in the Barents Sea. The causes of the by-catches during the new trap fishery for commercial crustaceans in the Barents Sea have been analyzed and discards from the catches have been estimated. The main portion of discards is crab processing wastes, including the cephalothorax and internal organs and tissues placed in. According to estimations in 2006–2015 during the Russian trap fishery of red king crab and snow crab, the annual weight of discards ranged from 1.3 to 4.6 thou. t. These years about 2.8 thou. t of biological materials have been thrown back at sea annually. About 85 % of the total mass of wastes in 2006–2015 has been the red king crabs body parts. Due to the high abundance and biomass of the red king crab stock observed in the recent years accompanied by an increase of the snow crab commercial stock, some increase in the total mass of discards of two crabs can be expected, as well as some increase in the portion of discards from snow crab fishery. Current circumstances preventing the full processing of crabs catches are the technical limitations of fishing vessels, as well as the absence of onshore enterprises capable for processing waste from crab fishery. The full use of catches of the red king crab and the snow crab in the Barents Sea could provide the raw materials for production of food products, as well as a wide range of pharmaceuticals for humans and animals.

  1. Muskie Lunacy: does the lunar cycle influence angler catch of muskellunge (Esox masquinongy)?

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    Vinson, Mark R; Angradi, Ted R

    2014-01-01

    We analyzed angling catch records for 341,959 muskellunge (Esox masquinongy) from North America to test for a cyclic lunar influence on the catch. Using periodic regression, we showed that the number caught was strongly related to the 29-day lunar cycle, and the effect was consistent across most fisheries. More muskellunge were caught around the full and new moon than at other times. At night, more muskellunge were caught around the full moon than the new moon. The predicted maximum relative effect was ≈5% overall. Anglers fishing exclusively on the peak lunar day would, on average, catch 5% more muskellunge than anglers fishing on random days. On some lakes and at night, the maximum relative effect was higher. We obtained angler effort data for Wisconsin, Mille Lacs (MN), and Lake Vermilion (MN). For Lake Vermilion there was a significant effect of the lunar cycle on angler effort. We could therefore not conclude that the lunar effect on catch was due to an effect on fish behavior alone. Several factors affected the amount of variation explained by the lunar cycle. The lunar effect was stronger for larger muskellunge (>102 cm) than for smaller fish, stronger in midsummer than in June or October, and stronger for fish caught at high latitudes (>48°N) than for fish caught further south. There was no difference in the lunar effect between expert and novice muskellunge anglers. We argue that this variation is evidence that the effect of the lunar cycle on catch is mediated by biological factors and is not due solely to angler effort and reflects lunar synchronization in feeding. This effect has been attributed to variation among moon phases in lunar illumination, but our results do not support that hypothesis for angler-caught muskellunge.

  2. Terrestrial vegetation and aquatic chemistry influence larval mosquito abundance in catch basins, Chicago, USA

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    Gardner Allison M

    2013-01-01

    Full Text Available Abstract Background An important determinant of mosquito-borne pathogen transmission is the spatial distribution of vectors. The primary vectors of West Nile virus (WNV in Illinois are Culex pipiens Linnaeus (Diptera: Culicidae and Culex restuans Theobald. In urban environments, these mosquitoes commonly oviposit in roadside storm water catch basins. However, use of this habitat is inconsistent, with abundance of larvae varying significantly across catch basins at a fine spatial scale. Methods We tested the hypothesis that attributes of the biotic and abiotic environment contribute to spatial and temporal variation in production of mosquito vectors, characterizing the relationship between terrestrial vegetation and aquatic chemistry and Culex abundance in Chicago, Illinois. Larvae were sampled from 60 catch basins from June 14 to October 3, 2009. Density of shrubs and 14 tree genera surrounding the basins were quantified, as well as aquatic chemistry content of each basin. Results We demonstrate that the spatial pattern of Culex abundance in catch basins is strongly influenced by environmental characteristics, resulting in significant variation across the urban landscape. Using regression and machine learning techniques, we described landscape features and microhabitat characteristics of four Chicago neighborhoods and examined the implications of these measures for larval abundance in adjacent catch basins. The important positive predictors of high larval abundance were aquatic ammonia, nitrates, and area of shrubs of height Culex during the fruit-bearing periods and early senescent periods in August and September. Conclusions This study identifies environmental predictors of mosquito production in urban environments. Because an abundance of adult Culex is integral to efficient WNV transmission and mosquitoes are found in especially high densities near larval habitats, identifying aquatic sites for Culex and landscape features that promote

  3. Physiological response of some economically important freshwater salmonids to catch-and-release fishing

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    Wedemeyer, G.A.; Wydoski, R.S.

    2008-01-01

    Catch-and-release fishing regulations are widely used by fishery resource managers to maintain both the quantity and quality of sport fish populations. We evaluated blood chemistry disturbances in wild brook trout Salvelinus fontinalis, brown trout Salmo trutta, cutthroat trout Oncorhynchus clarkii, and Arctic grayling Thymallus arcticus that had been hooked and played for 1-5 min in waters of the intermountain western United States. A hatchery stock of brown trout was included for comparison. To assess time needed for recovery, additional test groups were played for 5 min and then released into net-pens, where they were held for up to 72 h. The osmoregulatory and metabolic disturbances associated with catch-and-release fishing under the conditions we tested were minimal and judged to be well within normal physiological tolerance limits. In fish that were held for recovery, the blood chemistry alterations that did occur appeared to be related to stress from confinement in the net-pens. Our results confirm the results of previous studies, showing that prerelease air exposure and handling cause more physiological stress than does either hooking per se or playing time. Fishery managers must be aware of the differences in the perceptions, attitudes, and values of different societal groups, some of which feel that catch-and-release fishing should be banned because it is cruel to the animals. On the basis of brain anatomy, it seems highly unlikely that fish experience pain in the same manner as humans experience it, because fish lack a neocortex, the brain structure that enables the sensation of pain in higher vertebrates. However, independent of the neurobiological argument, our results indicate that under conditions similar to those tested, fish subjected to catch and release are neither suffering nor particularly stressed. Improved education programs about the relatively benign physiological effects of catch-and-release fishing as a fishery management practice would

  4. Evaluating spatial and temporal variability in growth and mortality for recreational fisheries with limited catch data

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    Li, Yan; Wagner, Tyler; Jiao, Yan; Lorantas, Robert M.; Murphy, Cheryl

    2018-01-01

    Understanding the spatial and temporal variability in life-history traits among populations is essential for the management of recreational fisheries. However, valuable freshwater recreational fish species often suffer from a lack of catch information. In this study, we demonstrated the use of an approach to estimate the spatial and temporal variability in growth and mortality in the absence of catch data and apply the method to riverine smallmouth bass (Micropterus dolomieu) populations in Pennsylvania, USA. Our approach included a growth analysis and a length-based analysis that estimates mortality. Using a hierarchical Bayesian approach, we examined spatial variability in growth and mortality by assuming parameters vary spatially but remain constant over time and temporal variability by assuming parameters vary spatially and temporally. The estimated growth and mortality of smallmouth bass showed substantial variability over time and across rivers. We explored the relationships of the estimated growth and mortality with spring water temperature and spring flow. Growth rate was likely to be positively correlated with these two factors, while young mortality was likely to be positively correlated with spring flow. The spatially and temporally varying growth and mortality suggest that smallmouth bass populations across rivers may respond differently to management plans and disturbance such as environmental contamination and land-use change. The analytical approach can be extended to other freshwater recreational species that also lack of catch data. The approach could also be useful in developing population assessments with erroneous catch data or be used as a model sensitivity scenario to verify traditional models even when catch data are available.

  5. Catch history and status of the harbour seal (Phoca vitulina in Greenland

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    Aqqalu Rosving-Asvid

    2010-09-01

    Full Text Available The number of harbour seals (Phoca vitulina in West Greenland declined rapidly after the 1950s and the seals have now abandoned their traditional haulout locations along the Greenland west coast. However, in recent years, a previously undetected group of about 60-100 harbour seals has been observed approximately 80 km upstream in a large river, and some traditional hauloutlocations are still in use near the south-eastern tip of Greenland. A small number of harbour seals is caught annually far from any of these locations, indicating that other groups might live unnoticed. Catch statistics provide the best evidence of the presence and locations of these remnant harbour seals. Therefore efforts were made to validate the recent catch statistics and to describe the catch history for the past 60 years. The catch statistics were also used to estimate plausibleranges of past and present numbers of harbour seals based on the assumption that hunting has caused the observed decline. The total number of harbour seals in Greenland according to these estimates was about 3,000 in 1950 and fewer than 1,000 in 2007. The number of harbour seals caught in the southernmost part of Greenland has, unlike in the rest of Greenland, increased significantly in some of the recent years. This change seems to be related to changes in the amount of drift ice. Drift ice reduces the frequency of contact between hunters and harbour seals in South Greenland and above normal quantities of drift ice from the mid 1960s to the mid 1980s probably allowed these seals to increase in numbers. Record low inflow of drift ice in some of the recent years, however, has resulted in record high catches, which likely have reduced the seals again. The remaining harbour seals in Greenland are few and without protection these sealsare potentially in danger of extinction.

  6. QUESTIONS OF EVALUATION OF POSTNATAL GROWTH IN PRETERM CHILDREN PART 2. CATCH-UP GROWTH IN PRETERM INFANTS

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    A. F. Kiosov

    2014-01-01

    Full Text Available The term catch-up growth is now widely used in neonatology and pediatrics to describe the growth of preterm infants. Catch-up growth — is a compensatory strengthening of the body's growth after a period of slow growth. The lower the gestational age of the premature baby, the more intense catch-up growth in the future.Catch-up growth is considered an important criterion for evaluating nursing preterm infants. Formation of catch-up growth is seen as a favorable outcome. Evaluation of catch-up growth is carried out with the use of percentiles and standard deviations (Z-score. Catch-up growth has a positive effect on neurological outcome. Catch-up growth achieved if the growth rates are between 5 and 10 percentile. The values of growth are satisfactory if they are located at the 10th percentile and above. Catch-up growth is achieved with a standard deviation greater than minus 2 (Z-score. The optimal parameters are considered indicators located within two standard deviations of the mean.Estimation of the growth should be carried out optimally weekly. Growth curves help to determine when the catch-up growth begins. After discharge from the hospital catch-up growth continues. Catch-up growth occurs within the first year of life. The critical period for catch-up growth are considered the first 6 months. Subsequently, the growth rate decreases. The growth is not sufficient, if the values are below the 3 percentile. About 50% of the causes impaired growth is due to the shortage of supply. Premature babies can have problems with food and after discharge from the hospital. The very rapid growth may lead to the development of metabolic syndrome in later life. Low birth weight is associated with increased risk of hypertension, stroke, and diabetes. Compensatory growth often occurs with excessive deposition of fat. In assessing the growth necessary to accommodate the growth of muscle mass and fat mass. Maintaining optimal growth rate is important for the

  7. Influence of catch-up growth on abdominal fat distribution in very low birth weight children - cohort study.

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    Alves, João Guilherme; Vasconcelos, Sarita Amorim; de Almeida, Tais Sá; Lages, Raquel; Just, Eduardo

    2015-01-01

    A rapid catch-up growth in very low birth weight has been associated both with a higher height growth and a higher risk to metabolic disturbances, including insulin resistance and its consequences. Abdominal fat distribution in early postnatal life may play a role in these outcomes and can help in addressing this neonatal dilemma. This study aimed to compare abdominal fat distribution among very low birth weight (VLBW) children with and without rapid catch-up growth. A cohort study followed 86 VLBW (children born in Brazil, during the first 3 years of life. Rapid catch-up growth was considered as an increased in length >2 Z score during the first year of life. Abdominal subcutaneous and preperitoneal fat thickness was determined by ultrasound. χ²-Test and Student's t-test were used to compare the groups. A total of 79 VLBW children completed the study, of whom 22 (27.8%) showed rapid catch-up growth. Abdominal subcutaneous and preperitoneal fat thickness showed no differences among children with or without rapid catch-up growth at 3.3 mm vs. 3.8 mm, respectively (p=0.79) and 4.0 mm vs. 4.0 mm (p=0.55), respectively. VLBW children with rapid catch-up growth were also taller. Rapid catch-up growth during the first year of life in VLBW children does not seem to change abdominal fat distribution until the third year of life.

  8. Present and future potential habitat distribution of Carcharhinus falciformis and Canthidermis maculata by-catch species in the tropical tuna purse-seine fishery under climate change

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    Nerea eLezama Ochoa

    2016-03-01

    Full Text Available By-catch species from tropical tuna purse seine fishery have been affected by fishery pressures since the last century; however, the habitat distribution and the climate change impacts on these species are poorly known. With the objective of predicting the potential suitable habitat for a shark (Carcharhinus falciformis and a teleost (Canthidermis maculata in the Indian, Atlantic and Eastern Pacific Oceans, a MaxEnt species distribution model (SDM was developed using data collected by observers in tuna purse seiners. The relative percentage of contribution of some environmental variables (depth, sea surface temperature, salinity and primary production and the potential impact of climate change on species habitat by the end of the century under the A2 scenario (scenario with average concentrations of carbon dioxide of 856 ppm by 2100 were also evaluated. Results showed that by-catch species can be correctly modelled using observed occurrence records and few environmental variables with SDM. Results from projected maps showed that the equatorial band and some coastal upwelling regions were the most suitable areas for both by-catch species in the three oceans in concordance with the main fishing grounds. Sea surface temperature was the most important environmental variable which contributed to explain the habitat distribution of the two species in the three oceans in general. Under climate change scenarios, the largest change in present habitat suitability is observed in the Atlantic Ocean (around 16% of the present habitat suitability area of Carcharhinus falciformis and Canthidermis maculata, respectively whereas the change is less in the Pacific (around 10% and 8% and Indian Oceans (around 3% and 2 %. In some regions such as Somalia, the Atlantic equatorial band or Peru’s coastal upwelling areas, these species could lose potential habitat whereas in the south of the equator in the Indian Ocean, the Benguela System and in the Pacific coast of

  9. Seasonal Variations of Oceanographic Variables and Eastern Little Tuna (Euthynnus affinis) Catches in the North Indramayu Waters Java Sea

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    Syamsuddin, Mega; Sunarto; Yuliadi, Lintang

    2018-02-01

    The remotely derived oceanographic variables included sea surface temperature (SST), chlorophyll-a (Chl-a) and Eastern Little Tuna (Euthynnus affinis) catches are used as a combined dataset to understand the seasonal variation of oceanographic variables and Eastern Little Tuna catches in the north Indramayu waters, Java Sea. The fish catches and remotely sensed data were analysed for the 5 years datasets from 2010-2014. This study has shown the effect of monsoon inducing oceanographic condition in the study area. Seasonal change features were dominant for all the selected oceanographic parameters of SST and Chl-a, and also Eastern Little Tuna catches, respectively. The Eastern Little Tuna catch rates have the peak season from September to December (700 to 1000) ton that corresponded with the value of SST ranging from 29 °C to 30 °C following the decreasing of Chl-a concentrations in September to November (0.4 to 0.5) mg m-3. The monsoonal system plays a great role in determining the variability of oceanographic conditions and catch in the north Indramayu waters, Java Sea. The catches seemed higher during the northwest monsoon than in the southeast monsoon for all year observations except in 2010. The wavelet spectrum analysis results confirmed that Eastern Little Tuna catches had seasonal and inter-annual variations during 2012-2014. The SST had seasonal variations during 2010-2014. The Chl-a also showed seasonal variations during 2010-2011 and interannual variations during 2011-2014. Our results would benefit the fishermen and policy makers to have better management for sustainable catch in the study area.

  10. Cost-effectiveness analysis of catch-up hepatitis A vaccination among unvaccinated/partially-vaccinated children

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    Hankin-Wei, Abigail; Rein, David B.; Hernandez-Romieu, Alfonso; Kennedy, Mallory J.; Bulkow, Lisa; Rosenberg, Eli; Trigg, Monica; Nelson, Noele P.

    2017-01-01

    Background Since 2006, the US Centers for Disease Control and Prevention has recommended hepatitis A (HepA) vaccination routinely for children aged 12–23 months to prevent hepatitis A virus (HAV) infection. However, a substantial proportion of US children are unvaccinated and susceptible to infection. We present results of economic modeling to assess whether a one-time catch-up HepA vaccination recommendation would be cost-effective. Methods We developed a Markov model of HAV infection that followed a single cohort from birth through death (birth to age 95 years). The model compared the health and economic outcomes from catch-up vaccination interventions for children at target ages from two through 17 years vs. outcomes resulting from maintaining the current recommendation of routine vaccination at age one year with no catch-up intervention. Results Over the lifetime of the cohort, catch-up vaccination would reduce the total number of infections relative to the baseline by 741 while increasing doses of vaccine by 556,989. Catch-up vaccination would increase net costs by $10.2 million, or $2.38 per person. The incremental cost of HepA vaccine catch-up intervention at age 10 years, the midpoint of the ages modeled, was $452,239 per QALY gained. Across age-cohorts, the cost-effectiveness of catch-up vaccination is most favorable at age 12 years, resulting in an Incremental Cost-Effectiveness Ratio of $189,000 per QALY gained. Conclusions Given the low baseline of HAV disease incidence achieved by current vaccination recommendations, our economic model suggests that a catch-up vaccination recommendation would be less cost-effective than many other vaccine interventions, and that HepA catch-up vaccination would become cost effective at a threshold of $50,000 per QALY only when incidence of HAV rises about 5.0 cases per 100,000 population. PMID:27317459

  11. Catch-Up Growth Occurs after Diarrhea in Early Childhood123

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    Richard, Stephanie A.; Black, Robert E.; Gilman, Robert H.; Guerrant, Richard L.; Kang, Gagandeep; Lanata, Claudio F.; Mølbak, Kåre; Rasmussen, Zeba A.; Sack, R. Bradley; Valentiner-Branth, Palle; Checkley, William

    2014-01-01

    Diarrhea and linear growth faltering continue to burden low-income countries and are among the most important contributors to poor health during early childhood. Diarrhea is thought to adversely affect linear growth, but catch-up growth can occur if no additional insults are experienced. We sought to characterize catch-up growth in relation to diarrhea burden in a multisite dataset of 1007 children. Using longitudinal anthropometry and diarrheal surveillance data from 7 cohort studies in 4 countries, we examined the relation between diarrhea prevalence and growth in 3- to 6-mo periods using linear mixed-effect models. Growth during each period was calculated as a function of age using linear splines. We incorporated the longitudinal prevalence of diarrhea in both current and previous periods into the model. Diarrhea during the current period was associated with slower linear and ponderal growth. Faster (catch-up) growth in length was observed in children with no diarrhea in age groups immediately after an age group in which diarrhea was experienced [age group >6–12 mo: 0.03 mm/mo for each percentage diarrhea prevalence in the previous period (95% CI: 0.007, 0.06) relative to 11.3 mm/mo mean growth rate; age group >12–18 mo: 0.04 mm/mo (95% CI: 0.02, 0.06) relative to 8.9 mm/mo mean growth rate; age group >18–24 mo: 0.04 mm/mo (95% CI: 0.003, 0.09) relative to 7.9 mm/mo mean growth rate]. The associations were stronger in boys than in girls when separate models were run. Similar results were observed when weight was the outcome variable. When diarrheal episodes are followed by diarrhea-free periods in the first 2 y of life, catch-up growth is observed that may allow children to regain their original trajectories. The finding of a greater effect of diarrhea on linear growth in boys than in girls was unexpected and requires additional study. Diarrhea burdens are high throughout the first 2 y of life in these study sites, therefore reducing the likelihood of

  12. Genomic amplification patterns of human telomerase RNA gene and C-MYC in liquid-based cytological specimens used for the detection of high-grade cervical intraepithelial neoplasia

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    Chen Shaomin

    2012-04-01

    Full Text Available Abstract Background The amplification of oncogenes initiated by high-risk human papillomavirus (HPV infection is an early event in cervical carcinogenesis and can be used for cervical lesion diagnosis. We measured the genomic amplification rates and the patterns of human telomerase RNA gene (TERC and C-MYC in the liquid-based cytological specimens to evaluate the diagnostic characteristics for the detection of high-grade cervical lesions. Methods Two hundred and forty-three residual cytological specimens were obtained from outpatients aged 25 to 64 years at Qilu Hospital, Shandong University. The specimens were evaluated by fluorescence in situ hybridization (FISH using chromosome probes to TERC (3q26 and C-MYC (8q24. All of the patients underwent colposcopic examination and histological evaluation. A Chi-square test was used for categorical data analysis. Results In the normal, cervical intraepithelial neoplasia grade 1 (CIN1, grade 2 (CIN2, grade 3 (CIN3 and squamous cervical cancer (SCC cases, the TERC positive rates were 9.2%, 17.2%, 76.2%, 100.0% and 100.0%, respectively; the C-MYC positive rates were 20.7%, 31.0%, 71.4%, 81.8% and 100.0%, respectively. The TERC and C-MYC positive rates were higher in the CIN2+ (CIN2, CIN3 and SCC cases than in the normal and CIN1 cases (p p p > 0.05. Conclusions The TERC test is highly sensitive and is therefore suitable for cervical cancer screening. The C-MYC test is not suitable for cancer screening because of its lower sensitivity. The amplification patterns of TERC become more diverse and complex as the severity of cervical diseases increases, whereas for C-MYC, the amplification patterns are similar between the normal/CIN1 and CIN2+ groups. Virtual slides The virtual slide(s for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1308004512669913.

  13. c-Myc Represses Tumor-Suppressive microRNAs, let-7a, miR-16 and miR-29b, and Induces Cyclin D2-Mediated Cell Proliferation in Ewing's Sarcoma Cell Line.

    Directory of Open Access Journals (Sweden)

    Masanori Kawano

    Full Text Available Myc oncogenic transcription factor is known to inhibit tumor suppressive microRNAs (miRNAs, resulting in greater expression of their target protein related to cell cycle, invasion or anti-apoptotic factors in human cancer cells. To explore possible oncogenic factors in Ewing's sarcoma (ES, we conducted microarray-based approach to profile the changes in the expression of miRNAs and its downstream mRNAs in five ES cell lines and human mesenchymal stem cells (hMSCs. Three miRNAs, let-7a, miR-16 and miR-29b were significantly down-regulated, whereas c-Myc and cyclin D2 (CCND2 were significantly up-regulated in all tested ES cells compared with hMSCs. To verify that let-7a, miR-16 and miR-29b were the targets of c-Myc in ES cell lines, we transfected siRNA against c-Myc and confirmed the coordinate up-regulation of let-7a, miR-16 and miR-29b through the repression of c-Myc. The ES cells transfected with c-Myc-siRNA and let-7a, miR-16 and miR-29b exhibited the inhibition of the cell cycle progression. The increased expression of let-7a, miR-16 and miR-29b resulted in the reduction of CCND2 protein expression. We also demonstrated that c-Myc-siRNA treatment of ES cells was associated with the decreased expression of CCND2 as a down-stream of three miRNAs. Furthermore, the introduction of let-7a, miR-16 and miR-29b in ES cells could inhibit the c-Myc-mediated up-regulation of CCND2 resulted in the prevention of cell cycle progression. In addition, the transfection of let-7a, miR-16 and miR-29b in ES cells suppressed tumor growth ex vivo treatment. These findings suggests that the up-regulation of c-Myc inhibited the expression of let-7a, miR-16 and miR-29b subsequently induced CCND2 expression in ES cells. The present study might identify a novel oncogenic axis that c-Myc regulates the expression of CCND2 via let-7a, miR-16 and miR-29b, leading to the development new therapeutic targets for ES.

  14. A preliminary survey of whale shark Rhincodon typus catch and trade in China: an emerging crisis.

    Science.gov (United States)

    Li, W; Wang, Y; Norman, B

    2012-04-01

    This study gives an account of spatial and temporal distribution of whale shark Rhincodon typus catch events in China on the basis of historical records and information obtained from interviews with fishing industry stakeholders. A total of 186 R. typus were recorded with key harvest areas identified as in Hainan and Zhejiang, and the peak catching seasons were May to June and September to October. Aspects of the R. typus trade are discussed, including products, markets and the process. The results suggest that R. typus is increasingly becoming a targeted resource in China as a consequence of fierce competition for large shark fins and an emerging local market for consumption of all body parts. Current obstacles and potential measures for sustainable exploitation and trade of R. typus are discussed. © 2012 The Authors. Journal of Fish Biology © 2012 The Fisheries Society of the British Isles.

  15. Catch-Quota Management - an example of result based fisheries management

    DEFF Research Database (Denmark)

    Kirkegaard, Eskild; Schou, Mogens; Dalskov, Jørgen

    The European Commission tabled in July 2011 a proposal for a revision of the Common Fisheries Policy (CFP). A central element of the revision is the introduction of a quota management system where all fish caught count against the quotas. The principle of full accountability of the catch...... is a complete change from present management where only the fish landed count against the quotas. The principle of full accountability opens for the introduction of a result based fisheries management strategy, where a fishery is regulated by clearly stated results and the fisher enjoys a maximum of freedom...... to plan and conduct the fishing operation to meet these results. Experiences from Danish trials with Catch Quota Management (CQM) conducted in 2008 to 2012 is presented and the possibilities of simplifying the current EU fisheries management and control regulations under a CQM system are evaluated...

  16. Downstream collecting in ciliary suspension feeders: the catch-up principle

    DEFF Research Database (Denmark)

    Riisgård, Hans Ulrik; Nielsen, Claus; Larsen, Poul Scheel

    2000-01-01

    -size retention spectrum, the lower limit depends on spacing between cilia in phase, while the upper end depends on cilia length which may or may not allow particles to enter the ciliary region. On the basis of fluid mechanical considerations and literature descriptions of structure and function of the ciliary......Based on observations of feeding structures and currents in the polychaete Spirorbis tridentatus, the entoproct Loxosoma pectinaricola and the cycliophore Symbion pandora, which all possess compound cilia, it is hypothesized that their capture mechanism is based on the catch-up principle. According...... to this principle, the compound cilia constitute the pump which generates a flow with suspended particles that enters the ciliary region. In this region the same cilia, during their power stroke, catch up with suspended particles and transfer the particles to a food groove, or a mouth cavity. In the particle...

  17. Catch-slip bonds can be dispensable for motor force regulation during skeletal muscle contraction

    Science.gov (United States)

    Dong, Chenling; Chen, Bin

    2015-07-01

    It is intriguing how multiple molecular motors can perform coordinated and synchronous functions, which is essential in various cellular processes. Recent studies on skeletal muscle might have shed light on this issue, where rather precise motor force regulation was partly attributed to the specific stochastic features of a single attached myosin motor. Though attached motors can randomly detach from actin filaments either through an adenosine triphosphate (ATP) hydrolysis cycle or through "catch-slip bond" breaking, their respective contribution in motor force regulation has not been clarified. Here, through simulating a mechanical model of sarcomere with a coupled Monte Carlo method and finite element method, we find that the stochastic features of an ATP hydrolysis cycle can be sufficient while those of catch-slip bonds can be dispensable for motor force regulation.

  18. Using continuation-ratio logits to analyze the variation of the age composition of fish catches

    DEFF Research Database (Denmark)

    Kvist, Trine; Gislason, Henrik; Thyregod, Poul

    2000-01-01

    Major sources of information for the estimation of the size of the fish stocks and the rate of their exploitation are samples from which the age composition of catches may be determined However, the age composition in the catches often varies as a result of several factors. Stratification...... of the sampling is desirable, because it leads to better estimates of the age composition, and the corresponding variances and covariances. The analysis is impeded by the fact that the response is ordered categorical. This paper introduces an easily applicable method to analyze such data. The method combines...... be applied separately to each level of the logits. The method is illustrated by the analysis of age-composition data collected from the Danish sandeel fishery in the North Sea in 1993. The significance of possible sources of variation is evaluated, and formulae for estimating the proportions of each age...

  19. Catch of pelagic hauls in Mediterranean acoustic surveys: Is it the same between day and night?

    Directory of Open Access Journals (Sweden)

    Athanassios Machias

    2013-03-01

    Full Text Available Fish sampling is a critical aspect of acoustic surveys, because it is directly related to the “transformation” of echo into species biomass and subsequently affects the accuracy of acoustic estimates. In the present study, we investigated the differences between day and night sampling in a the catch composition through certain diversity indices and b the length frequency distribution of anchovy and sardine using catch data of pelagic hauls collected from four different regions of the European Mediterranean waters. In addition, the possible bias in trawl efficiency due to sampling time and the possible error introduced in acoustic estimates were investigated. No statistically significant differences were found between day and night in any of the parameters examined. The results showed that a more flexible strategy can be adopted to reduce the duration and the cost of acoustic sampling for small pelagic species. The advantages and disadvantages of the two sampling strategies are discussed.

  20. Evaluation of catch-and-release regulations on Brook Trout in Pennsylvania streams

    Science.gov (United States)

    Jason Detar,; Kristine, David; Wagner, Tyler; Greene, Tom

    2014-01-01

    In 2004, the Pennsylvania Fish and Boat Commission implemented catch-and-release (CR) regulations on headwater stream systems to determine if eliminating angler harvest would result in an increase in the number of adult (≥100 mm) or large (≥175 mm) Brook Trout Salvelinus fontinalis. Under the CR regulations, angling was permitted on a year-round basis, no Brook Trout could be harvested at any time, and there were no tackle restrictions. A before-after–control-impact design was used to evaluate the experimental regulations. Brook Trout populations were monitored in 16 treatment (CR regulations) and 7 control streams (statewide regulations) using backpack electrofishing gear periodically for up to 15 years (from 1990 to 2003 or 2004) before the implementation of the CR regulations and over a 7–8-year period (from 2004 or 2005 to 2011) after implementation. We used Poisson mixed models to evaluate whether electrofishing catch per effort (CPE; catch/100 m2) of adult (≥100 mm) or large (≥175 mm) Brook Trout increased in treatment streams as a result of implementing CR regulations. Brook Trout CPE varied among sites and among years, and there was no significant effect (increase or decrease) of CR regulations on the CPE of adult or large Brook Trout. Results of our evaluation suggest that CR regulations were not effective at improving the CPE of adult or large Brook Trout in Pennsylvania streams. Low angler use, high voluntary catch and release, and slow growth rates in infertile headwater streams are likely the primary reasons for the lack of response.

  1. Is co-invention expediting technological catch up? A study of collaboration between emerging country firms

    OpenAIRE

    Giuliani, Elisa; Martinelli, Arianna; Rabellotti, Roberta

    2015-01-01

    Firms from emerging countries such as Brazil, India, and China (BIC) are going global, and Europe is attracting around one-third of their direct outward investments. Growing internationalization constitutes an opportunity for technological catch up. In this paper we analyze BIC firms' cross-border inventions with European Union (EU-27) actors, during the period 1990-2012. Our results suggest that cross-border inventions represent an opportunity for BIC firms to accumulate technological capabi...

  2. Long-term Trends in Catch Composition from Elasmobranch Derbies in Elkhorn Slough, California

    OpenAIRE

    Carlisle, Aaron

    2007-01-01

    Long-term trends in the elasmobranch assemblage of Elkhorn Slough, Monterey Bay, California, were analyzed by documenting species composition and catch per unit effort (CPUE) from 55 sport fishing derbies that occurred during May, June, and July, from 1951 until 1995. The most abundant species (bat ray, Myliobatis californica; shovelnose guitarfish, Rhinobatos productus; and leopard shark, Triakis semifasciata) were also analyzed for size-weight relationships, trends in size class distributio...

  3. Stranding and incidental catch of sea turtles in the coastal Tumbes, Peru

    Directory of Open Access Journals (Sweden)

    Carlos A. Rosales

    2011-05-01

    Full Text Available Strandings and incidental catches of four sea turtles species (Chelonia mydas, Lepidochelys olivacea, Dermochelys coriacea and Eretmochelys imbricata were registered in Tumbes Region since August 2007 to August 2009. These registers (52.6% of strandings and 47.4% of incidental catches occurred during all year; most frequently in Punta Picos (50.5%, Canoas (20.0% and Baja de Punta Mero (14.7%. The most registered species were C. mydas (64.2% and L. olivacea (30.5%; their sizes did not present significant differences between areas and climatic seasons. The higher percentage of C. mydas, L. olivacea and D. coriacea were considered sub-adults, including the only specimen of E. imbricata. The incidental catches were made with gillnets of different mesh sizes, but 8 inches mesh was most frequently. A high proportions of specimens were died with signs of drowning (22.2% this was due to the prolonged time of soak time of gillnet (approximately 12 hours. No significant differences in CPUE were found between climatic seasons and no seasonal pattern was evident. Lesions in 14% of stranded specimens were caused possibly by human attacks or by collisions with fishing boats. 77.8% of incidental catch specimens were sacrificed for the commercialization of his meat, and sometimes of his shell, this shows the lack of awareness of conservation. These observations indicate that the coast of Tumbes is an important foraging area and development of sub-adult specimens of sea turtles; so it is recomend to develop monitoring, awareness and critical areas protection programs to foment the conservation of these organisms in the Eastern Pacific.

  4. Big catch, little sharks: Insight into Peruvian small-scale longline fisheries

    OpenAIRE

    Doherty, Philip D; Alfaro-Shigueto, Joanna; Hodgson, David J; Mangel, Jeffrey C; Witt, Matthew J; Godley, Brendan J

    2014-01-01

    Shark take, driven by vast demand for meat and fins, is increasing. We set out to gain insights into the impact of small-scale longline fisheries in Peru. Onboard observers were used to document catch from 145 longline fishing trips (1668 fishing days) originating from Ilo, southern Peru. Fishing effort is divided into two seasons: targeting dolphinfish (Coryphaena hippurus; December to February) and sharks (March to November). A total of 16,610 sharks were observed caught, with 11,166 identi...

  5. Using variance structure to quantify responses to perturbation in fish catches

    Science.gov (United States)

    Vidal, Tiffany E.; Irwin, Brian J.; Wagner, Tyler; Rudstam, Lars G.; Jackson, James R.; Bence, James R.

    2017-01-01

    We present a case study evaluation of gill-net catches of Walleye Sander vitreus to assess potential effects of large-scale changes in Oneida Lake, New York, including the disruption of trophic interactions by double-crested cormorants Phalacrocorax auritus and invasive dreissenid mussels. We used the empirical long-term gill-net time series and a negative binomial linear mixed model to partition the variability in catches into spatial and coherent temporal variance components, hypothesizing that variance partitioning can help quantify spatiotemporal variability and determine whether variance structure differs before and after large-scale perturbations. We found that the mean catch and the total variability of catches decreased following perturbation but that not all sampling locations responded in a consistent manner. There was also evidence of some spatial homogenization concurrent with a restructuring of the relative productivity of individual sites. Specifically, offshore sites generally became more productive following the estimated break point in the gill-net time series. These results provide support for the idea that variance structure is responsive to large-scale perturbations; therefore, variance components have potential utility as statistical indicators of response to a changing environment more broadly. The modeling approach described herein is flexible and would be transferable to other systems and metrics. For example, variance partitioning could be used to examine responses to alternative management regimes, to compare variability across physiographic regions, and to describe differences among climate zones. Understanding how individual variance components respond to perturbation may yield finer-scale insights into ecological shifts than focusing on patterns in the mean responses or total variability alone.

  6. Genetic Evidence Highlights Potential Impacts of By-Catch to Cetaceans

    Science.gov (United States)

    Mendez, Martin; Rosenbaum, Howard C.; Wells, Randall S.; Stamper, Andrew; Bordino, Pablo

    2010-01-01

    Incidental entanglement in fishing gear is arguably the most serious threat to many populations of small cetaceans, judging by the alarming number of captured animals. However, other aspects of this threat, such as the potential capture of mother-offspring pairs or reproductive pairs, could be equally or even more significant but have rarely been evaluated. Using a combination of demographic and genetic data we provide evidence that i) Franciscana dolphin pairs that are potentially reproductive and mother-offspring pairs form temporal bonds, and ii) are entangled simultaneously. Our results highlight potential demographic and genetic impacts of by-catch to cetacean populations: the joint entanglement of mother-offspring or reproductive pairs, compared to random individuals, might exacerbate the demographic consequences of by-catch, and the loss of groups of relatives means that significant components of genetic diversity could be lost together. Given the social nature of many odontocetes (toothed cetaceans), we suggest that these potential impacts could be rather general to the group and therefore by-catch could be more detrimental than previously considered. PMID:21179542

  7. Assessing environmental performance in the European Union: Eco-innovation versus catching-up

    International Nuclear Information System (INIS)

    Beltrán-Esteve, Mercedes; Picazo-Tadeo, Andrés J.

    2017-01-01

    This paper assesses environmental performance in the European Union (EU) using Luenberger productivity indicators, directional distance functions and Data Envelopment Analysis techniques. It considers four indicators of the pressures exerted by economic activity on the environment: global warming, tropospheric ozone formation, acidification and particulate formation. The change in environmental performance from the early 2000s onwards is decomposed at the levels of country and environmental pressure, and as the result of eco-innovations and catching-up with the best available environmental technologies; furthermore, we distinguish between the periods of economic growth (2001–07) and severe crisis (2007–13). Our main finding is that environmental performance improved in both periods, mainly fuelled by advances in environmental technology. Accordingly, environmental policies aimed at boosting catching-up are highly recommended, particularly in the newer member states that joined the EU from 2004 onwards, which perform further away from their respective environmental technological frontiers. In addition, re-establishing the pre-crisis eco-innovation investment levels would also be highly advisable in order to return to the rates of environmental technical progress registered in the expansion period. - Highlights: • Environmental performance has noticeably improved in the EU since the 2000's. • Eco-innovations have bolstered progress in environmental performance. • Policies aimed at boosting catching-up are highly recommended in the EU.

  8. Fishing effort and catch composition of urban market and rural villages in Brazilian Amazon.

    Science.gov (United States)

    Hallwass, Gustavo; Lopes, Priscila Fabiana; Juras, Anastacio Afonso; Silvano, Renato Azevedo Matias

    2011-02-01

    The management of small-scale freshwater fisheries in Amazon has been based usually on surveys of urban markets, while fisheries of rural villages have gone unnoticed. We compared the fishing characteristics (catch, effort and selectivity) between an urban market and five small villages in the Lower Tocantins River (Brazilian Amazon), downstream from a large reservoir. We recorded 86 and 601 fish landings in the urban market and villages, respectively, using the same methodology. The urban fishers showed higher catch per unit of effort, higher amount of ice (related to a higher fishing effort, as ice is used to store fish catches) and larger crew size per fishing trip, but village fishers had a higher estimated annual fish production. Conversely, urban and village fishers used similar fishing gear (gillnets) and the main fish species caught were the same. However, village fishers showed more diverse strategies regarding gear, habitats and fish caught. Therefore, although it underestimated the total amount of fish caught in the Lower Tocantins River region, the data from the urban market could be a reliable indicator of main fish species exploited and fishing gear used by village fishers. Monitoring and management should consider the differences and similarities between urban and rural fisheries, in Amazon and in other tropical regions.

  9. Improvement of red pepper yield and soil environment by summer catch aquatic crops in greenhouses

    Science.gov (United States)

    Du, X. F.; Wang, L. Z.; Peng, J.; Wang, G. L.; Guo, X. S.; Wen, T. G.; Gu, D. L.; Wang, W. Z.; Wu, C. W.

    2016-08-01

    To investigate effects of the rotation of summer catch crops on remediation retrogressed soils in continuous cropping, a field experiment was conducted. Rice, water spinach, or cress were selected as summer catch crops; bare fallow during summer fallow was used as the control group. Results showed that aquatic crops grown in summer fallow period could effectively reduce soil bulk density and pH, facilitate soil nutrient release, and improve soil physical and chemical properties compared with those grown in fallow period. Paddy-upland rotation could improve soil microbial members and increase bacterial and actinomycete populations; by contrast, paddy-upland rotation could reduce fungal populations and enhance bacterium-to-fungus ratio. Paddy-upland rotation could also actively promote activities of soil enzymes, such as urease, phosphatase, invertase, and catalase. The proposed paddy-upland rotation significantly affected the growth of red pepper; the yield and quality of the grown red pepper were enhanced. Summer catch crops, such as rice, water spinach, and cress significantly increased pepper yield in the following growing season by 15.4%, 10.2% and 14.0%, respectively, compared with those grown in fallow treatment. Therefore, the proposed paddy-upland crop rotation could be a useful method to alleviate continuous cropping problems involved in cultivating red pepper in greenhouses.

  10. THE CATCHING-UP DEVELOPMENT: THE ASEAN EXPERIENCE AND IMPORT SUBSTITUTION POLICY OF RUSSIA

    Directory of Open Access Journals (Sweden)

    Т Ю Шалденкова

    2017-12-01

    Full Text Available The experience of catching-up development of ASEAN countries becomes valuable in the conditions of the Russian of import substitution policy and “turn to the East” in geopolitics. The objective of the paper is revealing the prospects of native catching-up development based on the analysis of theoretical aspects of the model of “flying geese” and their practical application in the ASEAN countries and Russia. The paper presents the theoretical foundations of catching-up development in the framework of the Flying Geese paradigm in the works of K. Akamatsu, R. Vernon, K. Kojima and indicates its main stages. The author reviews the measures of industrial, trade and investment policy of the ASEAN countries on the initial stages of the model and the modern aspects of their economic development. The materials of statistical reports of IMF, UNCTAD, ASEAN, the statistical data of Federal state statistics department of Russia, Federal customs department of Russia, the programs of the Russian Government in the field of import substitution are studied. The achievements and problems of implementing the programs of import substitution in agriculture, industry, IT-technologies in Russia were analyzed. The study showed the factors, hindering the domestic development in the framework of the Flying Geese paradigm. The recommendations for solving existing problems include the stimulation of aggregate demand and the development of high-tech exports. Special attention was drawn to the possibilities of applying selected elements of the model in terms of territorial heterogeneity of Russia.

  11. Paper Fish and Policy Conflict: Catch Shares and Ecosystem-Based Management in Maine's Groundfishery

    Directory of Open Access Journals (Sweden)

    Jennifer F. Brewer

    2011-03-01

    Full Text Available The National Oceanic and Atmospheric Administration professes support for ecosystem-based fisheries management, as mandated by Congress in the Fishery Conservation and Management Act, and as endorsed by the Obama Administration's national ocean policy. Nonetheless, driving agency policies, including catch shares and fishing quotas, focus principally on individual species, diverting attention from ecosystem considerations such as habitat, migratory patterns, trophic relationships, fishing gear, and firm-level decision making. Environmental non-governmental organization (ENGO agendas manifest similar inconsistencies. A case study of Maine's groundfishery demonstrates implications of this policy conflict at the local level. There, multigenerational fishing villages have historically pursued diversified and adaptive livelihood strategies, supported by local ecological knowledge. This tradition is increasingly eroded by regulatory constraints, including catch shares. Field observation, interviews, survey data, and archival review reveal that industry-supported, ecosystem-focused proposals have been rejected by the New England Fishery Management Council, despite the apparent failure of single-species approaches to sustain fish populations, fished ecosystems, and fishing-dependent communities. The creation of groundfishery catch share sectors is likely to perpetuate industry consolidation and political entrenchment under more mobile capital, following precedent set by days-at-sea, and making area protections and gear restrictions less likely. Pending marine spatial planning efforts could enhance social-ecological resilience by creating new opportunities for transdisciplinary decision support, and broader public participation and accountability.

  12. Catch up growth and social capability in developing countries: A conceptual and measurement proposal

    Directory of Open Access Journals (Sweden)

    Martin Andersson

    2017-12-01

    Full Text Available While the income per capita in the developing world since the turn of the Millennium has grown faster than that of the developed world, the question whether there is an ongoing process of catching up between countries remains. The notion of income convergence has provided many insights into the sources for long-run growth but has largely neglected the role of social capabilities in economic development. By social capabilities we mean the qualification of the ‘theory of convergence’ which asserts that productivity growth rates  between countries tend to vary inversely with regard to productivity levels. The social capabilities approach holds that a country’s potential for rapid growth is strong when “it is technologically backward but socially advanced” (see Abramovitz, 1986:388. This means that the potential to catch up under globalization is strongest for countries in which social capabilities are developed to allow successful use of technologies and where institutional arrangements are conducive to economic progress. Yet there is no clear agreement in the literature on the main components of social capabilities or how to measure them. Our framework argues that the role of capabilities in catching up needs to understand them in terms of structural transformation, economic and social inclusion, state´s autonomy and accountability. Without progress in these dimensions within-country inequality may increase and might in turn lead to stagnating growth and slim prospects for global income convergence.

  13. The Potential Research of Catch Crop in Decrease Soil Nitrate Under Greenhouse Vegetable Production

    Directory of Open Access Journals (Sweden)

    YIN Xing

    2015-06-01

    Full Text Available In order to clarify the impact of catch crops on greenhouse vegetable soil nitrate, explore the mechanism of barrier and controll soil nitrogen leaching losses in greenhouse, and provide a theoretical basis for control nitrogen leaching and prevention of groundwater pollution, this study selected the traditional greenhouse vegetable rotation system in North China plain as research subjects, using field situ remediation technologies on deep-root planting catch crops in the vegetable fallow period by sweet corn, Achyranthes bidentata and white Chrysanthemum. The results showed that: nitrogen content and nitrogen uptake of sweet corn and sweet corn with Achyranthes bidentata intercropping were the highest, respectively 20.11 t·hm-2, 19.62 t·hm-2 and 240.34 kg·hm-2, 287.56 kg·hm-2, significantly higher than white Chrysanthemum. The density of root length and root dry weight decreased with soil depth in the profiles, root length density was demonstrated in order as: intercropping sweet corn> sweet corn> white Chrysanthemum> intercropping Achyranthes bidentata blume. The reduction of NO3--N of sweet corn reached 907.87 kg·hm-2 in soil profile 0~200 cm, significantly higher than sweet corn and hyssop intercropping and white Chrysanthemums. In the interim period of vegetable crop rotation, planting catch crops could effectively reduce nitrate accumulation in the soil, control the soil profile nitrate leaching down.

  14. Fishing Effort and Catch Composition of Urban Market and Rural Villages in Brazilian Amazon

    Science.gov (United States)

    Hallwass, Gustavo; Lopes, Priscila Fabiana; Juras, Anastacio Afonso; Silvano, Renato Azevedo Matias

    2011-02-01

    The management of small-scale freshwater fisheries in Amazon has been based usually on surveys of urban markets, while fisheries of rural villages have gone unnoticed. We compared the fishing characteristics (catch, effort and selectivity) between an urban market and five small villages in the Lower Tocantins River (Brazilian Amazon), downstream from a large reservoir. We recorded 86 and 601 fish landings in the urban market and villages, respectively, using the same methodology. The urban fishers showed higher catch per unit of effort, higher amount of ice (related to a higher fishing effort, as ice is used to store fish catches) and larger crew size per fishing trip, but village fishers had a higher estimated annual fish production. Conversely, urban and village fishers used similar fishing gear (gillnets) and the main fish species caught were the same. However, village fishers showed more diverse strategies regarding gear, habitats and fish caught. Therefore, although it underestimated the total amount of fish caught in the Lower Tocantins River region, the data from the urban market could be a reliable indicator of main fish species exploited and fishing gear used by village fishers. Monitoring and management should consider the differences and similarities between urban and rural fisheries, in Amazon and in other tropical regions.

  15. Applying a reservoir functional-zone paradigm to littoral bluegills: differences in length and catch frequency?

    Directory of Open Access Journals (Sweden)

    Nathan Ruhl

    2014-08-01

    Full Text Available Reservoirs exhibit gradients in conditions and resources along the transition from lotic to lentic habitat that may be important to bluegill ecology. The lotic–lentic gradient can be partitioned into three functional zones: the riverine, transitional, and lacustrine zones. We measured catch frequency and length of bluegills (Lepomis macrochirus captured along the periphery of these areas (i.e., in the littoral zone of each functional zone for four small reservoirs in Southeastern Ohio during the summer months of three years. Catch frequency differed between zones for two reservoirs, but these differences were not observed in other years. There was no relationship between reservoir zone and either standard length or catch frequency when the data for all reservoirs were pooled, but we did observe a bimodal length distribution in all reservoirs. A combination of ecological factors including inter and intraspecific competition, predation intensity, management practices, limnology, and assemblage complexity may be mitigating bluegill distribution and abundance in reservoirs. Therefore, a functional zone (categorical approach to understanding bluegill ecology in reservoirs may not be appropriate.

  16. [Survival of patients with primary central nervous system diffuse large B-cell lymphoma: impact of gene aberrations and protein overexpression of bcl-2 and C-MYC, and selection of chemotherapy regimens].

    Science.gov (United States)

    Yin, W J; Zhu, X; Yang, H Y; Sun, W Y; Wu, M J

    2018-01-08

    Objective: To investigate the impact of clinicopathological features, gene rearrangements and protein expression of bcl-6, bcl-2, C-MYC and chemotherapy regime on the prognosis of patients with primary central nervous system diffuse large B-cell lymphoma (PCNS-DLBCL). Methods: Thirty-three cases of PCNS-DLBCL diagnosed from January 2006 to December 2016 at Zhejiang Cancer Hospital were collected. The expression of CD10, bcl-6, bcl-2, MUM1 and MYC were detected by immunohistochemical staining (IHC). The presence of EB virus was detected by in situ hybridization(EBER). Copy number variation (ICN) and translocation status of bcl-6, bcl-2 and C-MYC genes were detected by fluorescence in situ hybridization (FISH). The relationship between the above indexes and the prognosis was analyzed by univariate, bivariate survival analysis and multiple Cox hazard regression analysis. Results: The study included 33 patients of PCNS-DLBCL, without evidence of primary or secondary immunodeficient disease. Male to female ratio was 1.36∶1.00, and the average age was 56 years. Twenty cases had single lesion while 13 had multiple lesions. Deep brain involvement was seen in 12 cases. All patients underwent partial or total tumor resection. Five patients received whole brain post-surgery radiotherapy, nine patients received high-dose methotrexate (HD-MTX) based chemotherapy, and 12 patients received whole-brain radiotherapy combined with HD-MTX based chemotherapy. Severn patients received no further treatment and rituximab was used in 8 patients. According to the Hans model, 27 cases were classified as non-GCB subtypes (81.8%). Bcl-2 was positive in 25 cases (75.8%, 25/33) and highly expressed in 8 (24.2%). MYC was positive in 12 cases (36.4%) and double expression of bcl-2 and MYC was seen in 6 cases. EBER positive rate was 10.0%(3/30), all of which had multiple lesions. Two bcl-6 gene translocations and 3 amplifications were found in 28 patients. Two translocations, 3 ICN or with both

  17. 76 FR 74757 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Comprehensive Annual Catch Limit...

    Science.gov (United States)

    2011-12-01

    ... including productivity and susceptibility analysis and multivariate statistical analysis were used to... species within the Comprehensive ACL Amendment. Complexes for species groups would be established using associations based on life history, catch statistics from commercial logbook and observer data, recreational...

  18. Incentivising selective fishing under catch quotas: using an FCube modelling approach to evaluate management options for North Sea mixed fisheries

    DEFF Research Database (Denmark)

    Condie, Harriet M.; Dolder, Paul J.; Catchpole, Thomas L.

    Reforms of EU Common Fisheries Policy will make fundamental changes to European fisheries management, including a discard ban with catch quotas for regulated species and management to achieve MSY. We evaluate the impact of these changes on revenue of North Sea demersal finfish fleets and fish...... stocks. With no change in behaviour, revenue is reduced by a mean of 31% compared to current management in the first year, but partly recovers by year 3, as fishing mortality is reduced and stocks increase. There are large differences in revenue changes between fleets, varying from -99% to +36...... in revenue create a strong incentive to avoid catching the limiting species, particularly if it is not a primary target. Selectivity changes that avoid 30% cod catch reduced the economic impact for some fleets in moving to catch quotas. Increased flexibility will therefore be important in maintaining...

  19. 77 FR 56791 - Main Hawaiian Islands Deep 7 Bottomfish Annual Catch Limits and Accountability Measures for 2012-13

    Science.gov (United States)

    2012-09-14

    ... Accountability Measures for 2012-13 AGENCY: National Marine Fisheries Service (NMFS), National Oceanic and... accountability measure will help prevent overfishing and ensure sustainable, long-term catches for fishermen...

  20. THE STRUCTURE AND YIELD LEVEL OF SWEET CORN DEPENDING ON THE TYPE OF WINTER CATCH CROPS AND WEED CONTROL METHOD

    Directory of Open Access Journals (Sweden)

    Robert Rosa

    2014-10-01

    Full Text Available Organic manuring is suggested to be necessary in sweet corn cultivation. It is not always possible to use farmyard manure due to economic, production or technical reasons. Catch crops used as green manures can be an alternative source of organic matter. The experiment was carried out in central-east Poland (52°06’N, 22°55’E, in years 2008–2011. The successive effect of winter catch crops (hairy vetch, white clover, winter rye, Italian ryegrass, winter turnip rape and the type of weed control on the growth and yielding of sweet corn was examined. The catch crops were sown in early September, incorporated in early May. The effect of the winter catch crops on yield was compared to the effect of FYM at a rate of 30 t·ha-1 and the control without organic manuring. The sweet corn was grown directly after organic fertilization. Three methods of weed control was used: Hw – hand weeding, twice during the growing period, GCM – herbicide Guardian CompleteMix 664 SE, immediately after sowing the seed corn, Z+T – a mixture of herbicides Zeagran 340 SE + Titus 25 WG, in the 3–4 leaf stage sweet corn. The highest yields of biomass were found for winter rye (35.5 t·ha-1 FM and 7.3 t·ha-1 DM, the most of macroelements accumulated winter turnip rape (480.2 kg N+P+K+Ca+Mg·ha-1. Generally, leguminous catch crops had similar to the FYM and better than non-leguminous catch crops yield-forming effect. The highest yield of marketable ears of sweet corn was obtained after FYM (14.4 t·ha-1 and after hairy vetch catch crop (14.0 t·ha-1. A similar yield-forming effect also had white clover and Italian ryegrass. The most of ears from 1 ha was achieved after white clover catch crop (59.3 tausend, similar after FYM and hairy vetch catch crop. The highest kernel yields were found after FYM (10.7 t·ha-1. The yields of kernel after hairy vetch and white clover catch crops were significantly higher than after non-leguminous catch crops. Z+T weed control

  1. Primary central nervous system diffuse large B-cell lymphoma shows an activated B-cell-like phenotype with co-expression of C-MYC, BCL-2, and BCL-6.

    Science.gov (United States)

    Li, Xiaomei; Huang, Ying; Bi, Chengfeng; Yuan, Ji; He, Hong; Zhang, Hong; Yu, QiuBo; Fu, Kai; Li, Dan

    2017-06-01

    Diffuse large B-cell lymphoma (DLBCL) is the most common non-Hodgkin lymphoma, whose main prognostic factor is closely related to germinal center B-cell-like subtype (GCB- DLBCL) or activated B-cell-like type (non-GCB-DLBCL). The most common type of primary central nervous system lymphoma is diffuse large B-cell type with poor prognosis and the reason is unclear. This study aims to stratify primary central nervous system diffuse large B-cell lymphoma (PCNS-DLBCL) according to the cell-of-origin (COO) and to investigate the multiple proteins expression of C-MYC, BCL-6, BCL-2, TP53, further to elucidate the reason why primary central nervous system diffuse large B-cell lymphoma possesses a poor clinical outcome as well. Nineteen cases of primary central nervous system DLBCL were stratified according to immunostaining algorithms of Hans, Choi and Meyer (Tally) and we investigated the multiple proteins expression of C-MYC, BCL-6, BCL-2, TP53. The Epstein-Barr virus and Borna disease virus infection were also detected. Among nineteen cases, most (15-17 cases) were assigned to the activated B-cell-like subtype, highly expression of C-MYC (15 cases, 78.9%), BCL-2 (10 cases, 52.6%), BCL-6 (15 cases, 78.9%). Unfortunately, two cases were positive for PD-L1 while PD-L2 was not expressed in any case. Two cases infected with BDV but no one infected with EBV. In conclusion, most primary central nervous system DLBCLs show an activated B-cell-like subtype characteristic and have multiple expressions of C-MYC, BCL-2, BCL-6 protein, these features might be significant factor to predict the outcome and guide treatment of PCNS-DLBCLs. Copyright © 2017 Elsevier GmbH. All rights reserved.

  2. Environmental DNA from Seawater Samples Correlate with Trawl Catches of Subarctic, Deepwater Fishes.

    Directory of Open Access Journals (Sweden)

    Philip Francis Thomsen

    Full Text Available Remote polar and deepwater fish faunas are under pressure from ongoing climate change and increasing fishing effort. However, these fish communities are difficult to monitor for logistic and financial reasons. Currently, monitoring of marine fishes largely relies on invasive techniques such as bottom trawling, and on official reporting of global catches, which can be unreliable. Thus, there is need for alternative and non-invasive techniques for qualitative and quantitative oceanic fish surveys. Here we report environmental DNA (eDNA metabarcoding of seawater samples from continental slope depths in Southwest Greenland. We collected seawater samples at depths of 188-918 m and compared seawater eDNA to catch data from trawling. We used Illumina sequencing of PCR products to demonstrate that eDNA reads show equivalence to fishing catch data obtained from trawling. Twenty-six families were found with both trawling and eDNA, while three families were found only with eDNA and two families were found only with trawling. Key commercial fish species for Greenland were the most abundant species in both eDNA reads and biomass catch, and interpolation of eDNA abundances between sampling sites showed good correspondence with catch sizes. Environmental DNA sequence reads from the fish assemblages correlated with biomass and abundance data obtained from trawling. Interestingly, the Greenland shark (Somniosus microcephalus showed high abundance of eDNA reads despite only a single specimen being caught, demonstrating the relevance of the eDNA approach for large species that can probably avoid bottom trawls in most cases. Quantitative detection of marine fish using eDNA remains to be tested further to ascertain whether this technique is able to yield credible results for routine application in fisheries. Nevertheless, our study demonstrates that eDNA reads can be used as a qualitative and quantitative proxy for marine fish assemblages in deepwater oceanic

  3. cAMP-Dependent Protein Kinase A (PKA)-Mediated c-Myc Degradation Is Dependent on the Relative Proportion of PKA-I and PKA-II Isozymes.

    Science.gov (United States)

    Liu, Qingyuan; Nguyen, Eric; Døskeland, Stein; Ségal-Bendirdjian, Évelyne

    2015-09-01

    The transcription factor c-Myc regulates numerous target genes that are important for multiple cellular processes such as cell growth and differentiation. It is commonly deregulated in leukemia. Acute promyelocytic leukemia (APL) is characterized by a blockade of granulocytic differentiation at the promyelocyte stage. Despite the great success of all-trans retinoic acid (ATRA)-based therapy, which results in a clinical remission by inducing promyelocyte maturation, a significant number of patients relapse due to the development of ATRA resistance. A significant role has been ascribed to the cAMP/cAMP-dependent protein kinase A (PKA) signaling pathway in retinoid treatment since PKA activation is able to restore differentiation in some ATRA-resistant cells and eradicate leukemia-initiating cells in vivo. In this study, using NB4 APL cell variants resistant to ATRA-induced differentiation, we reveal distinct functional roles of the two PKA isozymes, PKA type I (PKA-I) and PKA-type II (PKA-II), on the steady-state level of c-Myc protein, providing a likely mechanism by which cAMP-elevating agents can restore differentiation in ATRA maturation-resistant APL cells. Therefore, both the inhibition of c-Myc activity and the PKA-I/PKA-II ratio should be taken into account if cAMP-based therapy is considered in the clinical management of APL. Copyright © 2015 by The American Society for Pharmacology and Experimental Therapeutics.

  4. Suppression of c-Myc is involved in multi-walled carbon nanotubes' down-regulation of ATP-binding cassette transporters in human colon adenocarcinoma cells

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Zhaojing [Department of Pharmacology, School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, 430030 Wuhan (China); Xu, Yonghong [Institute of Ophthalmological Research, Department of Ophthalmology, Renmin Hospital of Wuhan University, 430060 Wuhan (China); Meng, Xiangning [School of Materials and Metallurgy, Northeastern University, Shenyang 110819 (China); Watari, Fumio [Department of Biomedical, Dental Materials and Engineering, Graduate School of Dental Medicine, Hokkaido University, Sapporo 060-8586 (Japan); Liu, Hudan, E-mail: hudanliu@hust.edu.cn [Department of Pharmacology, School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, 430030 Wuhan (China); Chen, Xiao, E-mail: mornsmile@yahoo.com [Department of Pharmacology, School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, 430030 Wuhan (China)

    2015-01-01

    Over-expression of ATP-binding cassette (ABC) transporters, a large family of integral membrane proteins that decrease cellular drug uptake and accumulation by active extrusion, is one of the major causes of cancer multi-drug resistance (MDR) that frequently leads to failure of chemotherapy. Carbon nanotubes (CNTs)-based drug delivery devices hold great promise in enhancing the efficacy of cancer chemotherapy. However, CNTs' effects on the ABC transporters remain under-investigated. In this study, we found that multiwalled carbon nanotubes (MWCNTs) reduced transport activity and expression of ABC transporters including ABCB1/Pgp and ABCC4/MRP4 in human colon adenocarcinoma Caco-2 cells. Proto-oncogene c-Myc, which directly regulates ABC gene expression, was concurrently decreased in MWCNT-treated cells and forced over-expression of c-Myc reversed MWCNTs' inhibitory effects on ABCB1 and ABCC4 expression. MWCNT-cell membrane interaction and cell membrane oxidative damage were observed. However, antioxidants such as vitamin C, β-mecaptoethanol and dimethylthiourea failed to antagonize MWCNTs' down-regulation of ABC transporters. These data suggest that MWCNTs may act on c-Myc, but not through oxidative stress, to down-regulate ABC transporter expression. Our findings thus shed light on CNTs' novel cellular effects that may be utilized to develop CNTs-based drug delivery devices to overcome ABC transporter-mediated cancer chemoresistance.

  5. The Child and Adolescent Trial for Cardiovascular Health (CATCH): intervention, implementation, and feasibility for elementary schools in the United States.

    Science.gov (United States)

    Perry, C L; Sellers, D E; Johnson, C; Pedersen, S; Bachman, K J; Parcel, G S; Stone, E J; Luepker, R V; Wu, M; Nader, P R; Cook, K

    1997-12-01

    The Child and Adolescent Trial for Cardiovascular Health (CATCH) was the largest school-based field trial ever sponsored by the National Institutes of Health. The trial demonstrated positive changes in the school food service and physical education program, as well as in students' cardiovascular health behaviors. Because the CATCH intervention programs were implemented in 56 schools (in four states) that were typical of schools throughout the United States, their reception by schools and degree of implementation provide evidence about their feasibility for schools nationally. Extensive process evaluation data were collected from students, teachers, school food service personnel, and physical education specialists throughout the three school years of the CATCH intervention. Four of the CATCH programs--school food service, physical education, classroom curricula, and home programs--were assessed over the three school years. The process data provide information on participation, dose, fidelity, and compatibility of the CATCH programs in the intervention schools for these programs. High levels of participation, dose, fidelity, and compatibility were observed for the four programs during the 3 school years. CATCH emerges as a model of a feasible multilevel health promotion program to improve eating and exercise behaviors for elementary schools in the United States.

  6. Creating Aerosol Types from CHemistry (CATCH): A New Algorithm to Extend the Link Between Remote Sensing and Models

    Science.gov (United States)

    Dawson, K. W.; Meskhidze, N.; Burton, S. P.; Johnson, M. S.; Kacenelenbogen, M. S.; Hostetler, C. A.; Hu, Y.

    2017-11-01

    Current remote sensing methods can identify aerosol types within an atmospheric column, presenting an opportunity to incrementally bridge the gap between remote sensing and models. Here a new algorithm was designed for Creating Aerosol Types from CHemistry (CATCH). CATCH-derived aerosol types—dusty mix, maritime, urban, smoke, and fresh smoke—are based on first-generation airborne High Spectral Resolution Lidar (HSRL-1) retrievals during the Ship-Aircraft Bio-Optical Research (SABOR) campaign, July/August 2014. CATCH is designed to derive aerosol types from model output of chemical composition. CATCH-derived aerosol types are determined by multivariate clustering of model-calculated variables that have been trained using retrievals of aerosol types from HSRL-1. CATCH-derived aerosol types (with the exception of smoke) compare well with HSRL-1 retrievals during SABOR with an average difference in aerosol optical depth (AOD) methods. In the future, spaceborne HSRL-1 and CATCH can be used to gain insight into chemical composition of aerosol types, reducing uncertainties in estimates of aerosol radiative forcing.

  7. The scope and structure of the production and catch of fish in Serbia in the period from 2006. to 2012.

    Directory of Open Access Journals (Sweden)

    Ivanović Jelena

    2015-01-01

    Full Text Available Fish represents a significant source of animal proteins in the diet of people in a large part of the world. The market in Serbia is supplied from its own production (carp and trout fish ponds and catch from open waters. However, the largest part of the fish on the market is imported. Average area under carp fish ponds from 2006. to 2012. was 8,417 hektars, and under trout fish ponds was 49,900 square meters. Average annual production of carp fish species in this period was 7,228 tons, and of trout was 923 tons. In open waters of Serbia, the catch (from both professional and sport fishing is averagely 3,745 tons of fish. The biggest catch of fish in open waters refers to goldfish, then carp and bream, while lower catch refers to white bighead. The objective of this investigation was to determine the scope and structure of fish catch and production in Serbia from 2006. to 2012. The data on fish catch and production were taken from Statistical Yearbook of the Republic of Serbia. [Projekat Ministarstva nauke Republike Srbije, br. TR 31011

  8. Controlled and localized delivery of c-myc AS-ODN to cells by 3-aminopropyl-trimethoxylsilane modified SBA-15 mesoporous silica

    Science.gov (United States)

    Zhang, Juan; Chen, Minmin; Zhao, Xiqiu; Zhang, Min; Mao, Jinxiang; Cao, Xichuan; Zhang, Zhuoqi

    2018-01-01

    SBA-15 mesoporous silicate was synthesized and functionalized with 3-aminopropyl organic groups through a post-synthesis method. The materials were characterized consecutively by powder X-ray diffraction (XRD), N2 adsorption/desorption analysis and solid-state magic-angle spinning 29Si nuclear magnetic resonance (MAS NMR). Human c-myc anti-sense oligodeoxyneucleotide (AS-ODN) was selected as a model molecule to be loaded onto the surface of bare and functionalized SBA-15 via different loading conditions. It has been found that the amount of AS-ODN incorporated into the porous matrix is strongly dependent on the surface properties, pH of the loading solvent and AS-ODN concentration. The release behaviour of AS-ODN from modified SBA-15 materials was also investigated and depended on conditions chosen. Cellular uptake of the eluted AS-ODN into Hela cells was observed by fluorescent microscopy. The materials showed excellent cytocompatibility. The AS-ODN keeps full transfection and expression activities indicating its structural integrity. The functionalized SBA-15 is an excellent prospect as a biomedical material candidate for the future.

  9. Thermo-Responsive Complexes of c-Myc Antisense Oligonucleotide with Block Copolymer of Poly(OEGMA) and Quaternized Poly(4-Vinylpyridine).

    Science.gov (United States)

    Topuzogullari, Murat; Elalmis, Yeliz Basaran; Isoglu, Sevil Dincer

    2017-04-01

    Solution behavior of thermo-responsive polymers and their complexes with biological macromolecules may be affected by environmental conditions, such as the concentration of macromolecular components, pH, ion concentration, etc. Therefore, a thermo-responsive polymer and its complexes should be characterized in detail to observe their responses against possible environments under physiological conditions before biological applications. To briefly indicate this important issue, thermo-responsive block copolymer of quaternized poly(4-vinylpyridine) and poly(oligoethyleneglycol methyl ether methacrylate) as a potential nonviral vector has been synthesized. Polyelectrolyte complexes of this copolymer with the antisense oligonucleotide of c-Myc oncogene are also thermo-responsive but, have lower LCST (lower critical solution temperature) values compared to individual copolymer. LCST values of complexes decrease with molar ratio of macromolecular components and presence of salt. Dilution of solutions also affects solution behavior of complexes and causes a significant decrease in size and an increase in LCST, which indicates possible effects of severe dilutions in the blood stream. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  10. Melatonin promotes circadian rhythm-induced proliferation through Clock/histone deacetylase 3/c-Myc interaction in mouse adipose tissue.

    Science.gov (United States)

    Liu, Zhenjiang; Gan, Lu; Luo, Dan; Sun, Chao

    2017-05-01

    Melatonin is synthesized in the pineal gland and controls circadian rhythm of peripheral adipose tissue, resulting in changes in body weight. Although core regulatory components of clock rhythmicity have been defined, insight into the mechanisms of circadian rhythm-mediated proliferation in adipose tissue is still limited. Here, we showed that melatonin (20 mg/kg/d) promoted circadian and proliferation processes in white adipose tissue. The circadian amplitudes of brain and muscle aryl hydrocarbon receptor nuclear translocator-like 1 (Bmal1, Pcircadian locomotor output cycles kaput (Clock, Pcircadian disruption and promoted adipocyte proliferation in chronic jet-lagged mice and obese mice. Thus, our study found that melatonin promoted adipocyte proliferation by forming a Clock/HDAC3/c-Myc complex and subsequently driving the circadian amplitudes of proliferation genes. Our data reveal a novel mechanism that links circadian rhythm to cell proliferation in adipose tissue. These findings also identify a new potential means for melatonin to prevent and treat sleep deprivation-caused obesity. © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  11. Promoter trans-activation of protooncogenes c-fos and c-myc, but not c-Ha-ras, by products of adenovirus early region 1A

    International Nuclear Information System (INIS)

    Sassone-Corsi, P.; Borrelli, E.

    1987-01-01

    The E1A (early region 1A) oncogene products of adenovirus type 2 trans-activate the other early viral transcription units, as well as some cellular promoters. Using a short-term cotransfection assay in murine NIH 3T3 fibroblasts, we show that c-fos and c-myc promoter activities are stimulated by the E1A proteins, whereas c-Ha-ras transcription is not affected. The product of E1A 13S mRNA is responsible for the trans-activation, whereas the 12S mRNA product has no effect. Analysis of the c-fos promoter sequences required for the E1A stimulation shows that responsive sequences are located between positions -402 and -240 upstream of the transcription initiation site. This same region also contains the c-fos serum-responsive element. Furthermore, transcription of the endogenous c-fos gene in HeLa cells is increased after E1A transfection

  12. Catching COBRAs

    NARCIS (Netherlands)

    Muntinga, D.G.

    2013-01-01

    With social media usage increasingly widespread and influential, companies face the challenge of inspiring and cultivating Consumers’ Online Brand-Related Activities (COBRAs). This dissertation argues that they can do so effectively only when they have a good understanding of consumers’ willingness

  13. Catching Butterflies

    Science.gov (United States)

    Fisher, Maisha T.

    2008-01-01

    Playmaking for Girls, founded by Rachel May and directed by Susie Spear Purcell, assembles a diverse ensemble of teaching artists committed to using playwriting and performance to help with incarcerated and formerly incarcerated teen girls to help them "think and thus act for themselves" (Freden, 2001, p. 70). These teaching artists, with the…

  14. Artisanal Green Turtle, Chelonia mydas, fishery of Caribbean Nicaragua: I. Catch rates and trends, 1991-2011.

    Directory of Open Access Journals (Sweden)

    Cynthia J Lagueux

    Full Text Available This is the first assessment of catch rates for the legal, artisanal green turtle, Chelonia mydas, fishery in Caribbean Nicaragua. Data were collected by community members, monitoring up to 14 landing sites from 1991 to 2011. We examined take levels, and temporal and spatial variability in catch rates for the overall fishery, by region, and community using General Additive Mixed Models (GAMMs. More than 171,556 green turtles were killed during the period, with a mean estimated minimum 8,169±2,182 annually. There was a statistically significant decline in catch rates overall. Catch rates peaked in 1997 and 2002, followed by a downward trend, particularly from mid-2008 to the end of the study period. Similar downward trends were evident in both study regions. Community specific catch rate trends also indicated declines with decreases ranging from 21% to 90%. Decrease in catch rates in Nicaragua is cause for concern even though the principal source rookery at Tortuguero, Costa Rica, shows an increase in nesting activity. Explanations for the apparent discrepancy between the increasing trend at Tortuguero and decreasing catch rate trends in Nicaragua include: i an increase in reproductive output, ii insufficient time has passed to observe the impact of the fishery on the rookery due to a time lag, iii changes in other segments of the population have not been detected since only nesting activity is monitored, iv the expansive northern Nicaragua foraging ground may provide a refuge for a sufficient portion of the Tortuguero rookery, and/or v a larger than expected contribution of non-Tortuguero rookeries occurring in Nicaragua turtle fishing areas. Our results highlight the need for close monitoring of rookeries and in-water aggregations in the Caribbean. Where consumptive use still occurs, nations sharing this resource should implement scientifically based limits on exploitation to ensure sustainability and mitigate impacts to regional population

  15. Biomass and energy production of catch crops in areas with deficiency of precipitation during summer period in central Bohemia

    International Nuclear Information System (INIS)

    Brant, V.; Pivec, J.; Fuksa, P.; Neckar, K.; Kocourkova, D.; Venclova, V.

    2011-01-01

    The biomass production dynamics of catch crops, volunteers and weeds in dependence on precipitation and air temperature, was studied in central Bohemia from 2004 to 2006. The cover of individual components of the growth was monitored during the same period. Also measured were energy and efficiency of utilization of global radiation by catch crops and volunteers. The catch crops included the following species: Brassica napus, Lolium multiflorum, Lolium perenne, Phacelia tanacetifolia, Sinapis alba, Trifolium incarnatum, Raphanus sativus var. oleiformis and Trifolium subterraneum. The highest biomass production and the highest cover of catch crops were observed in treatments with S. alba (1382.0 kg ha -1 , 47.8%). The average biomass production (sum of catch crops, volunteers and weeds) was highest in treatments with S. alba, R. sativus, and P. tanacetifolia and lowest in treatments with B. napus, L. multiflorum and L. perenne. It was demonstrated that an increase in the percentage share of volunteers caused a decrease in the biomass production of catch crops. The average energy production ranged from 0.31 to 2.37 MJ m -2 in treatments with catch crops, and from 0.25 to 0.89 MJ m -2 in treatments with cereal volunteers. The highest effectivity of global radiation utilization, was determined in treatments with S. alba (0.11-0.47%). Based on regression analysis the closest dependence between biomass production from all treatments on the experimental site and precipitation was observed from 1st May till the time of sowing and the average air temperatures from the sowing period till the time of the last biomass production assessment.

  16. Body Composition and Catch-up in Height after Treatment of Malnourished Children

    International Nuclear Information System (INIS)

    Badaloo, Asha; Morris, Richard; Forrester, Terrence; Reid, Marvin; Wootton, Steve; Jackson, Alan

    2014-01-01

    Full text: Background and Objectives: The use of enriched diets to promote rapid catch-up-growth and the delay in catch-up in length/height relative to catch-up in weight may be associated with excess adiposity and poor lean tissue deposition. We aimed to assess lean mass (LM) during treatment of Jamaican malnourished children and to investigate if stunted malnourished children remained stunted as adults. Methods: All participants were managed in hospital and in accordance with WHO guidelines. Diagnosis (marasmus, kwashiorkor, marasmic-kwashiorkor) was based on the Welcome Classification. LM was estimated from total body water(TBW)in 44 children (group 1), aged 3 to 32 months, during the stabilization period, mid catch-up growth and on achieving at least 90% of reference weight-for-length using deuterium dilution technique. Length-for-age (LAZ) and height-for-age (HAZ) Z-scores were determined in another group of 168 malnourished children (group 2) on admission, aged 3 to 33 months, and when they attained adulthood. LAZ was calculated using recent WHO child growth standards. Results: Data are mean ± SD. The participants included moderately and severely malnourished children based on weight-for-length Z-score (WLZ). In group 1, after initial stabilization intake increased to 685 ± 101 kJ/kg/d and 3.7 ± 0.6 g protein/kg/d resulting in rapid weight gain (16.5 ± 5.1 g/kg/d) and catch-up in weight over 20 ± 10 d. Consequently, WLZ at admission (non-oedematous: -3.0 ± 0.2; oedematous: -2.1 ± 0.2) improved to -0.48 ± 0.2. At recovery in weight, LM was consistent with reported values in normal children (71% to 92 % of body weight and 11 to 15 kg/m2) but the children remained stunted (LAZ: -3.3 ± 3.2). Insecurity of TBW measurement and estimating LM in the acute malnourished state and during rapid growth limits the ability to capture changes in lean and fat during rehabilitation. Similar to group 1, WLZ in group 2 was -3.2 ± 1.3 on admission; -0.6 ± 1 at recovery

  17. Regulation of Long Bone Growth in Vertebrates; It Is Time to Catch Up.

    Science.gov (United States)

    Roselló-Díez, Alberto; Joyner, Alexandra L

    2015-12-01

    The regulation of organ size is essential to human health and has fascinated biologists for centuries. Key to the growth process is the ability of most organs to integrate organ-extrinsic cues (eg, nutritional status, inflammatory processes) with organ-intrinsic information (eg, genetic programs, local signals) into a growth response that adapts to changing environmental conditions and ensures that the size of an organ is coordinated with the rest of the body. Paired organs such as the vertebrate limbs and the long bones within them are excellent models for studying this type of regulation because it is possible to manipulate one member of the pair and leave the other as an internal control. During development, growth plates at the end of each long bone produce a transient cartilage model that is progressively replaced by bone. Here, we review how proliferation and differentiation of cells within each growth plate are tightly controlled mainly by growth plate-intrinsic mechanisms that are additionally modulated by extrinsic signals. We also discuss the involvement of several signaling hubs in the integration and modulation of growth-related signals and how they could confer remarkable plasticity to the growth plate. Indeed, long bones have a significant ability for "catch-up growth" to attain normal size after a transient growth delay. We propose that the characterization of catch-up growth, in light of recent advances in physiology and cell biology, will provide long sought clues into the molecular mechanisms that underlie organ growth regulation. Importantly, catch-up growth early in life is commonly associated with metabolic disorders in adulthood, and this association is not completely understood. Further elucidation of the molecules and cellular interactions that influence organ size coordination should allow development of novel therapies for human growth disorders that are noninvasive and have minimal side effects.

  18. Computer-aided tracking and characterization of homicides and sexual assaults (CATCH)

    Science.gov (United States)

    Kangas, Lars J.; Terrones, Kristine M.; Keppel, Robert D.; La Moria, Robert D.

    1999-03-01

    When a serial offender strikes, it usually means that the investigation is unprecedented for that police agency. The volume of incoming leads and pieces of information in the case(s) can be overwhelming as evidenced by the thousands of leads gathered in the Ted Bundy Murders, Atlanta Child Murders, and the Green River Murders. Serial cases can be long term investigations in which the suspect remains unknown and continues to perpetrate crimes. With state and local murder investigative systems beginning to crop up, it will become important to manage that information in a timely and efficient way by developing computer programs to assist in that task. One vital function will be to compare violent crime cases from different jurisdictions so investigators can approach the investigation knowing that similar cases exist. CATCH (Computer Aided Tracking and Characterization of Homicides) is being developed to assist crime investigations by assessing likely characteristics of unknown offenders, by relating a specific crime case to other cases, and by providing a tool for clustering similar cases that may be attributed to the same offenders. CATCH is a collection of tools that assist the crime analyst in the investigation process by providing advanced data mining and visualization capabilities.These tools include clustering maps, query tools, geographic maps, timelines, etc. Each tool is designed to give the crime analyst a different view of the case data. The clustering tools in CATCH are based on artificial neural networks (ANNs). The ANNs learn to cluster similar cases from approximately 5000 murders and 3000 sexual assaults residing in a database. The clustering algorithm is applied to parameters describing modus operandi (MO), signature characteristics of the offenders, and other parameters describing the victim and offender. The proximity of cases within a two-dimensional representation of the clusters allows the analyst to identify similar or serial murders and sexual

  19. Technical Suitability and Static Stability of Sungkur Fishing Boats for Fish and Shrimp Catching

    Science.gov (United States)

    Rusmilyansari; Rosadi, E.; Iriansyah

    2017-10-01

    Sungkur fishing gear is operated actively on one the side of fishing boat, which requires technical suitability and fishing gear stability to ensure success in fish catching. This is a case study which aimed to analyze some technical issues related to the boat, boat’s hydrostatic parameters, and the boat’s stability. The data were collected though observation, measuring the boat to obtain the offset table. The data were analyzed numerically and descriptively. The data were processed with technical formula, Microsoft Office’s Excel software, graphic display, minitab, statistical data processing, and maxsurf program. The research results showed that: (1) the sungkur fishing boat dimensional ratio L/B (6.47 - 7.00); L/D (10.90 - 11.20) and B/D (1.60 - 1.668) is within the range value of Indonesian fishing boats suitable to operate the fishing gear by towing or dragging. However, during fish catching operation, there have been problems in a hydrodynamic force due to the fishing gear movement, which affect the fish catching efficiency. (2) The boat’s coefficient of fineness is in the fine type shape; the displacement on each waterline has increased; the loads of the boat are getting larger following the increase of waterline from one to five; this is also shown from the increasing midship area value. Ton per centimeter immersion to change wl 1 by 1 cm needs 0.04 tons of weight. (3) Sungkur fishing boat have a good static stability, which is proven by the positive value of angle of maximum GZ by 79.1 - 83.6. In other words, the boat has the ability to return to its original position after tilting; however, stability dynamics happens because fishing gear operation are located on just one side of boat.

  20. Implementing Attachment and Biobehavioral Catch-Up with birth parents: Rationale and case example.

    Science.gov (United States)

    Hoye, Julie R; Dozier, Mary

    2018-05-25

    Attachment and Biobehavioral Catch-Up (ABC) is an intervention designed for vulnerable children and their parents. This intervention enhances parental sensitivity and nurturance with the goal of promoting secure, organized attachments and strong self-regulatory capabilities among children. Here, we provide a brief rationale for the need for such interventions to be delivered to parent-child dyads in the child welfare system. Next, we review specific intervention targets of ABC. We include a case example of two birth parents and their daughter who became involved in Child Protective Services due to domestic violence. © 2018 Wiley Periodicals, Inc.

  1. Sensory Properties of Frozen Herring (Clupea harengus) from Different Catch Seasons and Locations

    DEFF Research Database (Denmark)

    Hyldig, Grethe; Jørgensen, Bo Munk; Undeland, Ingrid

    2012-01-01

    Freezing of herring (Clupea harengus) for human consumption is increasing in the Nordic herring industry, either onboard the fishing vessels or right after landing. The quality of frozen herring as a raw material does not only depend on the frozen storage conditions applied, but also...... on compositional features, something which in turn can vary with season and catching ground. To unravel the link between biological variations, basic muscle composition, and sensory properties of frozen herring, a unique herring raw material was caught by commercial fishing vessels at three locations: around...... the utilization of herring for frozen storage for human consumption....

  2. Safety catching device for pipe lines in missile shielding cylinders of nuclear power plants

    International Nuclear Information System (INIS)

    Hering, S.; Doll, B.

    1975-01-01

    The safety catching device for pipes in the missile shielding cylinders consists of a flexible steel cable surrounding the pipe in a distance in U-shape. The arrester cable - which works as a spring and is freely movable in all directions - is attached to the cylinder wall. For this, the ends of the cable are primarily fastened to anchor boxes which are then inserted in a stay tube with the same axis as the cable ends. The anchor boxes are fastened to the outer wall of the missile shielding cylinder by anchor bolts and holding plates. (DG/AK) [de

  3. Professor Gerschenkron goes to Brussels. Russian Catch-up Economics and the Common European Space

    Directory of Open Access Journals (Sweden)

    Stefan Hedlund

    2006-06-01

    Full Text Available Ongoing discussions between Russia and the EU on the formation of a Common European Economic Space bring back to mind Alexander Gerschenkron's classic essay on economic backwardness in historical perspective. This paper argues that the institutions that once produced a specific kind of catch-up economics in Czarist Russia still remain largely the same. Unless negotiations between Moscow and Brussels take into consideration such fundamental institutional incompatibility, attempts at harmonization, expressed by Brussels as an attempt tp spread Western values, will be doomed to fail. A cynical conlusion views potential convergence as adaptation by Brussels to traditional Russian institutional patterns of rule evasion, rather than a Westernization of Russia

  4. Catch-Up: A Rule That Makes Service Sports More Competitive

    OpenAIRE

    Brams, Steven J.; Ismail, Mehmet S.; Kilgour, D. Marc; Stromquist, Walter

    2016-01-01

    Service sports include two-player contests such as volleyball, badminton, and squash. We analyze four rules, including the Standard Rule (SR), in which a player continues to serve until he or she loses. The Catch-Up Rule (CR) gives the serve to the player who has lost the previous point—as opposed to the player who won the previous point, as under SR. We also consider two Trailing Rules that make the server the player who trails in total score. Surprisingly, compared with SR, only CR gives th...

  5. Catch-Up:A Rule that Makes Service Sports More Competitive

    OpenAIRE

    Brams, Steven; Ismail, Mehmet; Kilgour, Marc; Stromquist, Walter

    2018-01-01

    Service sports include two-player contests such as volleyball, badminton, and squash. We analyze four rules, including the Standard Rule (SR), in which a player continues to serve until he or she loses. The Catch-Up Rule (CR) gives the serve to the player who has lost the previous point—as opposed to the player who won the previous point, as under SR. We also consider two Trailing Rules that make the server the player who trails in total score. Surprisingly, compared with SR, only CR gives th...

  6. Trade-Based Estimation of Bluefin Tuna Catches in the Eastern Atlantic and Mediterranean, 2005–2011

    Science.gov (United States)

    Gagern, Antonius; van den Bergh, Jeroen; Sumaila, Ussif Rashid

    2013-01-01

    The Eastern Atlantic and Mediterranean stock of Bluefin tuna Thunnus thynnus (BFTE) has long been considered overfished and at risk of collapse. Although ICCAT quotas for this stock have decreased considerably over the past years, uncertainty exists about the degree of catch beyond this quota. The extent of such catch is an important piece of information in stock assessment models as well as being an indicator of the effectiveness of fisheries management. We present a model using Bluefin tuna trade data to infer actual catches. Basing our calculations on 25 countries involved in BFTE trade, we estimate that between 2005 and 2011, allowable quotas were exceeded by 44 percent. This gap between catch and quotas has slightly increased over past years, leading to estimated excess catches of 57 percent for the period between 2008 and 2011. To improve assessments, preparation and design of BFTE management, we suggest that the estimated total removals reported in this paper be included in stock assessment models for BFTE. An implication of our findings is that ICCAT member states should take stronger measures to monitor and enforce compliance with quotas. PMID:23922870

  7. Management practices utilization in organizations – A comparison between catching-up and well-developed economies

    Directory of Open Access Journals (Sweden)

    Vojko Potočan

    2016-01-01

    Full Text Available The main purpose of this paper is to examine the utilization of management practices in catching-up and well-developed economies. Within that framework, the paper examines the utilization of management practices in Slovenian and Croatian organizations, as both are considered the representatives of catching-up economies from Central and East Europe (CEE, while also comparing the utilization of management practices in the two selected economies to the global use of management practices. Evidences about management practices utilization reveal that in well-developed economies in the forefront are management practices aiming to support customer relationship management, strength customer relations and customer satisfaction, while in Slovenia and Croatia prevail practices that are oriented toward optimization of the business and its processes. Based on the research results and the situation in organizations, a possible future pattern of management practice utilization in organizations operating in catching up economies is outlined, with the aim to reduce the developmental lag and increase competitiveness of those organizations. The paper also provides several practical implications for future utilization of management practices in catching-up economies. The sample of catching up-countries includes 155 responses from employees in Slovenian organizations and 185 from Croatian ones. Data for samples from well-developed economies are obtained from a global survey of management practices.

  8. BIOLOGY AND CPUE SPATIAL DISTRIBUTION OF ESCOLAR Lepidocybium flavobrunneum (Smith, 1843 IN EASTERN INDIAN OCEAN (EVOLVING FISHERIES: TODAY’S BY-CATCH IS TOMORROW’S TARGET CATCH

    Directory of Open Access Journals (Sweden)

    Fathur Rochman

    2016-12-01

    Full Text Available Discharge of by catch is a significant problem in world fishery. Every commercial fishery such as tuna longline has a suite of bycatch species, escolar fish (LEC. LEC as by catch product has received a little attention because of its lower economic value and given its importance as a secondary market. With time, however, market can become establish for this presently undesirable species. Acknowledging that today’s by catch might become tomorrow’s target fish. The aims of this study areto provide information on biological aspect and catch per unit of effort (CPUE spatial distribution of escolar (Lepidocybium flavobrunneum as by catch in Indonesian longline fishery operating in the Eastern Indian Ocean. Total escolar samples of 1,815 were taken from scientific observer data from 2011-2013. The study area of escolar was between 0.897-33.175°S and 85.366– 138.733°E of Eastern Indian Ocean. Results show that the escolar length (cmFL is distributed from 27-178 cmFL (median=83 cmFL, mode=85 cmFL, mean=83.95 cmFL and n= 1.812 and dominated by the size of 85 cmFL. The length weight relationship was determined to be W=0.0002FL2.2926(W in kg, FL in cm. In terms of CPUEs distribution, the lower CPUEs(1.0001 to 7.382 generally occurred in Western Australian, precisely on grid between 10-35°S and 85-110°E. These grids would be a potential for fishing LEC with the best time to catch in June to August.

  9. Diel variation in the catch of fishes and penaeid shrimps in a tropical estuary

    Science.gov (United States)

    Stoner, Allan W.

    1991-07-01

    In Laguna Joyuda, Puerto Rico, 24-h trawl surveys were conducted to test the hypothesis that there is no diel variation in the catch rate of fishes and penaeid shrimps in a turbid, microtidal estuary relatively free of visual predation. The null hypothesis was not rejected for the two most abundant fishes in the lagoon, Eucinostomus gula (Gerreidae) and Achirus lineatus (Bothidae). Conversely, the total number of fishes, Diapterus rhombeus (Gerreidae), and the engraulids Anchoa cubana and A. hepsetus were collected in significantly higher numbers during hours of darkness. Total penaeid catch was significantly higher in night than in day collections. The most abundant species were Penaeus notialis and P. subtilis, both of which appeared to be strong daytime burrowers. Small penaeids demonstrated greater diel variation in trawl capture rate than larger (subadult) shrimps. Diel variation in capture rate was related to habitat associations of individual species. Fauna resident in the very turbid lagoon demonstrated no diel variation in activity patterns and trawl susceptibility. Those species which spend only the early part of their lives in the lagoon, or live in both turbid and clear water, had significant diel variation in activity and trawl catchability.

  10. Miniature and micro spectrometers market: who is going to catch the value?

    Science.gov (United States)

    Bouyé, Clémentine; d'Humières, Benoît

    2017-02-01

    The market of miniature and micro spectrometers is evolving fast. The technology is getting ever smaller and cheaper while keeping high performances. The market is attracting new players: spin-offs from major research institutes, large companies outside the classic spectroscopy market, software providers with innovative analytical solutions, … The goal of this involvement is to bring spectroscopy closer to the end-users and provide spectrometers able to operate on-field or in-line. The high potential of compact spectrometers is recognized for a wide variety of applications: chemistry, pharmaceutics, agro-food, agriculture, forensics, healthcare, consumer applications, … But its emergence as a large volume market faces a major bottleneck. Each application implies specific processes and analyses and specific parameters to control, i.e. a specific interpretation of the raw spectra in order to provide information usable by nonphotonic experts. Who is going to pay for that adaptation effort? Are there ways for reducing the adaptation costs, by means of selflearning algorithms and/or flexible and easily adaptable sensors? In other words, who is going to catch the value? In this article, we will investigate the potential of each major industrial application market and provide market data. We will also wonder, what are the strengths and weaknesses of the different players - spectrometer manufacturers, algorithms developers, full-systems providers, … - to catch the value of the compact spectrometer market.

  11. Evaluating methodological assumptions of a catch-curve survival estimation of unmarked precocial shorebird chickes

    Science.gov (United States)

    McGowan, Conor P.; Gardner, Beth

    2013-01-01

    Estimating productivity for precocial species can be difficult because young birds leave their nest within hours or days of hatching and detectability thereafter can be very low. Recently, a method for using a modified catch-curve to estimate precocial chick daily survival for age based count data was presented using Piping Plover (Charadrius melodus) data from the Missouri River. However, many of the assumptions of the catch-curve approach were not fully evaluated for precocial chicks. We developed a simulation model to mimic Piping Plovers, a fairly representative shorebird, and age-based count-data collection. Using the simulated data, we calculated daily survival estimates and compared them with the known daily survival rates from the simulation model. We conducted these comparisons under different sampling scenarios where the ecological and statistical assumptions had been violated. Overall, the daily survival estimates calculated from the simulated data corresponded well with true survival rates of the simulation. Violating the accurate aging and the independence assumptions did not result in biased daily survival estimates, whereas unequal detection for younger or older birds and violating the birth death equilibrium did result in estimator bias. Assuring that all ages are equally detectable and timing data collection to approximately meet the birth death equilibrium are key to the successful use of this method for precocial shorebirds.

  12. Behaviour of fish by-catch in the mouth of a crustacean trawl.

    Science.gov (United States)

    Queirolo, D; Gaete, E; Montenegro, I; Soriguer, M C; Erzini, K

    2012-06-01

    The behaviour of fish by-catch was recorded and characterized by in situ observations in the mouth of a crustacean trawl using an underwater camera system with artificial light, at depths between 106 and 461 m, along the central coast of Chile. The groups or species studied were rattails (family Macrouridae), Chilean hake Merluccius gayi gayi, sharks (orders Carcharhiniformes and Squaliformes), skates (family Rajidae), flatfishes (genus Hippoglossina) and small benthopelagic and demersal fishes (orders Osmeriformes, Stomiiformes, Gadiformes, Ophidiiformes and Perciformes). The fish behaviour was categorized in terms of (1) position in the water column, (2) initial orientation with respect to the trawl, (3) locomotion and (4) swimming speed with respect to the trawl. Rattails, sharks, skates and flatfishes were passive in response to the trawl and showed similar behavioural patterns, with most fishes observed sitting or touching the bottom with no swimming or other activity. Merluccius gayi gayi was the most active species, displaying a wide combination of behavioural responses when the trawl approached. This species showed several behavioural patterns, mainly characterized by swimming forward at variable speed. A fraction of small bentho-pelagic and demersal fishes also showed an active behaviour but always at lower speed than the trawl. The species-specific differences in behaviour in the mouth of the trawl suggest that improvements at the level of the footrope can be made to reduce by-catch, especially of passive species. © 2012 The Authors. Journal of Fish Biology © 2012 The Fisheries Society of the British Isles.

  13. Should total landings be used to correct estimated catch in numbers or mean-weight-at-age?

    DEFF Research Database (Denmark)

    Lewy, Peter; Lassen, H.

    1997-01-01

    Many ICES fish stock assessment working groups have practised Sum Of Products, SOP, correction. This correction stems from a comparison of total weights of the known landings and the SOP over age of catch in number and mean weight-at-age, which ideally should be identical. In case of SOP...... discrepancies some countries correct catch in numbers while others correct mean weight-at-age by a common factor, the ratio between landing and SOP. The paper shows that for three sampling schemes the SOP corrections are statistically incorrect and should not be made since the SOP is an unbiased estimate...... of the total landings. Calculation of the bias of estimated catch in numbers and mean weight-at-age shows that SOP corrections of either of these estimates may increase the bias. Furthermore, for five demersal and one pelagic North Sea species it is shown that SOP discrepancies greater than 2% from...

  14. THE EFFECT OF WINTER CATCH CROPS ON WEED INFESTATION IN SWEET CORN DEPENDING ON THE WEED CONTROL METHODS

    Directory of Open Access Journals (Sweden)

    Robert Rosa

    2015-02-01

    Full Text Available An experiment was carried out in east-central Poland (52°06’ N, 22°55’ E over 2008–2011 to study the effect of winter catch crops on the weed infestation, number, and fresh matter of weeds in sweet corn (Zea mays L. var. saccharata. The following winter catch crops were grown: hairy vetch (Vicia villosa Roth., white clover (Trifolium repens L., winter rye (Secale cereale L., Italian ryegrass (Lolium multiflorum L. and winter turnip rape (Brassica rapa var. typica Posp.. The catch crops were sown in early September and incorporated in early May. The effect of the catch crops was compared to the effect of FYM (30 t·ha-1 and control without organic manuring (NOM. Three methods of weed control were used: HW – hand weeding, twice during the growing period, GCM – the herbicide Guardian Complete Mix 664 SE, immediately after sowing of corn seeds, Z+T – a mixture of the herbicides Zeagran 340 SE and Titus 25 WG applied at the 3–4-leaf stage of sweet corn growth. Rye and turnip rape catch crops had least weeds in their fresh matter. Sweet corn following winter catch crops was less infested by weeds than corn following farmyard manure and non-manured corn. Least weeds and their lowest weight were found after SC, BRT and VV. LM and BRT reduced weed species numbers compared with FYM and NOM. The greatest weed species diversity, determined at the corn flowering stage, was determined after SC and FYM. The number and weight of weeds were significantly lower when chemically controlled compared with hand weeding. The best results were observed after a post-emergent application of the mixture Z+T. The weed species diversity on Z+T-treated plots was clearly lower compared with GCM and HW.

  15. Management implications of the biodiversity and socio-economic impacts of shrimp trawler by-catch in Bahía de Kino, Sonora, México.

    Science.gov (United States)

    Meltzer, Lorayne; Blinick, Naomi S; Fleishman, Abram B

    2012-01-01

    The shrimp fishery is the most economically important fishery in Mexico. The trawler-based portion of this fishery results in high rates of by-catch. This study quantifies and describes the biodiversity of by-catch associated with trawling in the Bahía de Kino region of Sonora, Mexico. Data were collected from 55 trawls, on six boats, over 14 nights, during November of 2003, 2004, 2006-2009. By-catch rates within trawl samples averaged 85.9% measured by weight. A total of 183 by-catch species were identified during the course of this study, including 97 species of bony fish from 43 families, 19 species of elasmobranchs from 12 families, 66 species of invertebrates from eight phyla, and one species of marine turtle; seven of the documented by-catch species are listed on the IUCN Red List, CITES, or the Mexican NOM-059-ECOL-2010; 35 species documented in the by-catch are also targeted by local artisanal fishers. Some of the species frequently captured as juveniles in the by-catch are economically important to small-scale fishers in the region, and are particularly sensitive to overexploitation due to their life histories. This study highlights the need for further research quantifying the impacts of high levels of by-catch upon small-scale fishing economies in the region and presents strong ecological and economic rationale for by-catch management within the shrimp fishery of the Gulf of California. Site-specific by-catch management plans should be piloted in the Bahía de Kino region to address the growing momentum in national and international fisheries policy regimes toward the reduction of by-catch in shrimp fisheries.

  16. Time for Men to Catch up on Women? A Study of the Swedish Gender Wage Gap 1973-2012

    OpenAIRE

    Löfström, Åsa

    2014-01-01

    The Swedish gender wage gap decreased substantially from the 1960s until the beginning of the 1980s. At the same time women had been narrowing men in employment experience and education. While women continued to catch up on men the average wage gap remained almost the same as in the 1980s. The catch-up hypothesis was obviously not the sole answer to the wage-gap. The purpose here was to discuss other factors of relevance for the evolution of the average pay gap. Data for the period 1972-2012 ...

  17. THE ILLUSION OF CATCHING UP THE SOCIO-ECONOMIC REGIONAL GAPS

    Directory of Open Access Journals (Sweden)

    Eugen\tGHIORGHITA

    2015-06-01

    Full Text Available There is a sort of scientific bias in the way economics literature approaches the issues of general or regional economic growth and socio-economic backwardness. The term 'catching up of socio-economic gaps' appears to be rather a contradictio in adjecto, a sort of oxymoron, than a possible evolution of the world’s countries. The very idea of ‘catching-up’ or ‘recovery’ as a consequence of ‘lagging behind’ seems to be more specific to sports competitions or post-surgery periods. Transfering the idea of ‘backwardness’ to human communities (be it tribes, villages or political-administrative entities of higher dimensions is at least questionable. The contradiction is generated by the very establishment of the benchmarks for identifying ‘backwardness’. During the last 50 years, and after 1990 especially, the appeal to such indicators as GDP/capita, HDI, Gini coefficient, convergence criteria etc. became a common place. Nowadays it appears to anyone a matter-of-course, that any geopolitical entity can be "weighed" or "measured" by reference to a set of uniform criteria. Unfortunately, the global and regional efforts made after WW II towards an accelerated socio-economic growth of both developing and less developed countries succeded only for few countries to reduce the historically shaped gaps as against developed countries. On the contrary, for the bulk of the ‘lagged behind’ regions gaps seem to have risen. This is why many questions arose about the inefficiency of the steps made towards 'catching up of socio- economic gaps', such as: “Why after about 70 years of international welfare levelling efforts, gaps between development and living standards of the world regions are still so large?” “Why do economies have to grow at any price?” “Is the forced (artificial, accelerated development beneficial? And it if so, to whom?” “Are specific historical socio-economic structures negligible under the pressure of

  18. The Coordination and Activity Tracking in CHildren (CATCH) study: rationale and design.

    Science.gov (United States)

    Cairney, John; Missiuna, Cheryl; Timmons, Brian W; Rodriguez, Christine; Veldhuizen, Scott; King-Dowling, Sara; Wellman, Sarah; Le, Tuyen

    2015-12-21

    Past studies have found that children with Developmental Coordination Disorder (DCD) engage in less physical activity than typically developing children. This "activity deficit" may result in children with DCD being less physically fit and more likely to be overweight or obese, potentially increasing later risk for poor cardiovascular health. Unfortunately, the majority of DCD research has been limited to cross-sectional designs, leading to questions about the complex relationship among motor ability, inactivity and health-related fitness. Of the few longitudinal studies on the topic, determining precedence amongst these factors is difficult because study cohorts typically focus on mid to late childhood. By this age, both decreased physical fitness and obesity are often established. The Coordination and Activity Tracking in CHildren (CATCH) study will examine the pathways connecting DCD, physical activity, physical fitness, and body composition from early to middle childhood. The CATCH study is a prospective cohort study. We aim to recruit a cohort of 600 children aged 4 to 5 years (300 probable DCD [pDCD] and 300 controls) and test them once a year for 4 years. At Phase 1 of baseline testing, we assess motor skills, cognitive ability (IQ), basic anthropometry, flexibility and lower body muscle strength, while parents complete an interview and questionnaires regarding family demographics, their child's physical activity, and behavioural characteristics. Children who move on to Phase 2 (longitudinal cohort) have their body fat percentage, foot structure, aerobic and anaerobic fitness assessed. An accelerometer to measure physical activity is then given to the child and interested family members. The family also receives an accelerometer logbook and 3-day food dairy. At years 2 to 4, children in the longitudinal cohort will have all baseline assessments repeated (excluding the IQ test), and complete an additional measure of perceived self-efficacy. Parents will

  19. Catch crops as universal and effective method for reducing nitrogen leaching loss in spring cereal production: A meta-analysis.

    Science.gov (United States)

    Valkama, Elena; Lemola, Riitta; Känkänen, Hannu; Turtola, Eila

    2016-04-01

    Sustainable farms produce adequate amounts of a high-quality product, protect their resources and are both environmentally friendly and economically profitable. Nitrogen (N) fertilization decisively influences the cereal yields as well as increases soil N balance (N input in fertilizer - N output in harvested yield), thereby leading to N losses to the environment. However, while N input reduction affects soil N balance, such approach would markedly reduce N leaching loss only in case of abnormally high N balances. As an alternative approach, the growing of catch crops aims to prevent nutrient leaching in autumn after harvest and during the following winter, but due to competition, catch crops may also reduce yields of the main crop. Although studies have explored the environmental effects of catch crops in cereal production in the Nordic countries (Denmark, Sweden, Finland and Norway) during the past 40 years, none has yet carried out a meta-analysis. We quantitatively summarized 35 studies on the effect of catch crops (non-legume and legume) undersown in spring cereals on N leaching loss or its risk as estimated by the content of soil nitrate N or its sum with ammonium in late autumn. The meta-analysis also included the grain yield and N content of spring cereals. To identify sources of variation, we studied the effects of soil texture and management (ploughing time, the amount of N applied, fertilizer type), as well as climatic (annual precipitation) and experimental conditions (duration of experiments, lysimeter vs. field experiments). Finally, we examined whether the results differed between the countries or over the decades. Compared to control groups with no catch crops, non-legume catch crops, mainly ryegrass species, reduced N leaching loss by 50% on average, and soil nitrate N or inorganic N by 35% in autumn. Italian ryegrass depleted soil N more effectively (by 60%) than did perennial ryegrass or Westerwolds ryegrass (by 25%). In contrast, legumes (white

  20. Nutrient cycling in a cropping system with potato, spring wheat, sugar beet, oats and nitrogen catch crops. II. Effect of catch crops on nitrate leaching in autumn and winter

    NARCIS (Netherlands)

    Vos, J.; Putten, van der P.E.L.

    2004-01-01

    The Nitrate Directive of the European Union (EU) forces agriculture to reduce nitrate emission. The current study addressed nitrate emission and nitrate-N concentrations in leachate from cropping systems with and without the cultivation of catch crops (winter rye: Secale cereale L. and forage rape:

  1. When access to drugs meets catch-up: Insights from the use of CL threats to improve access to ARV drugs in Brazil

    NARCIS (Netherlands)

    Ramani, Shyama V.; Urias, E.

    2018-01-01

    Access to affordable lifesaving medicines is considered a human right. This leads to a question largely understudied in the catch-up literature on accumulation of industrial capabilities. Can the need to improve access to an essential commodity impact the sectoral catch-up trajectory of the

  2. Effects of environmental variables on survey catch rates and distribution by size of shallow- and deep-water Cape hakes, Merluccius capensis and Merluccius paradoxus off Namibia

    DEFF Research Database (Denmark)

    Kainge, Paulus Inekela; van der Plas, A. K.; Bartholomae, C. H.

    2017-01-01

    In order to study the effects of temperature, oxygen, salinity and time of day on survey trawl catches, we modeled observed catches of juvenile, small, medium and large hakes per station as functions of zenith angle of the sun, geographical position, year, temperature, salinity, oxygen and depth....

  3. Innovation systems in small catching-up economies new perspectives on practice and policy

    CERN Document Server

    Varblane, Urmas; Roolaht, Tõnu

    2012-01-01

    In several parts of the world, countries are undergoing economic, social, and political transitions, enhanced and accelerated by the forces of globalization. These transition economies can serve as laboratories for understanding the innovation process. This volume features original theoretical and empirical research. It offers the first comprehensive view of innovation system development in the context of small catching-up economies. Smallness, path dependency, and latecomer status of such economies create some inherent limitations for their innovation systems, but these special characteristics can offer advantages as well. For example, smallness is often related with increased flexibility and shorter reaction times, while latecomers can benefit from earlier experiences of their more advanced neighbors. Path-dependency highlights the fact that the innovation system development processes are considerably influenced by the past experience of a particular country or region. By incorporating these features into a...

  4. The Catch-up Education Programme in Turkey: Opportunities and challenges

    Science.gov (United States)

    Börkan, Bengü; Ünlühisarcıklı, Özlem; Caner, H. Ayşe; Sart, Z. Hande

    2015-02-01

    Turkish children between the ages of 10-14 who either never enrolled in primary education, dropped out of school, or were at least three years behind their peers had the opportunity of joining an accelerated learning programme. It was developed by the Turkish Ministry of National Education General Directorate of Primary Education as a response to the urgent need to secure these children's right to education. The programme, called "Catch-up Education Programme" (CEP), was implemented between September 2008 and 2013 in collaboration with the United Nations Children's Fund (UNICEF) to enable those children to complete several grades in a short time and then to continue their education with their peers. This paper presents the findings of a study, conducted in 2010, reviewing the challenges and opportunities experienced during the implementation process from the points of view of beneficiaries (pupils and parents) and implementers. The results are discussed in relation to educational policy and its implementation in general.

  5. Strategic Entrepreneurship Based Model of Catch-up University in Global Rankings

    Directory of Open Access Journals (Sweden)

    Kozlov Mikhail

    2016-01-01

    Full Text Available The paper will help answer the question, why only few universities managed to succeed significantly in their global ranking advancement, while most of their competitors fail. For this purpose it will introduce a new strategically entrepreneurial catch-up university framework, based on the combination of the resource based view, dynamic capabilities, strategic entrepreneurship and latecomer organization concepts. The new framework logics explains the advantages of being ambidextrous for ranking oriented universities and pursuing new potentially more favorable opportunities for research development. It will propose that substantial increase in the level of dynamic capabilities of the universities and their resource base accumulation is based on the use of the new combination of financial, human and social capital combined with strategic management of these resources in the process of identification and exploitation of greater opportunities.

  6. SU-E-T-158: Catching Errors with Trajectory Log Files

    International Nuclear Information System (INIS)

    Wijesooriya, K; Seitter, K; Desai, V; Dharia, I; Read, P; Larner, J

    2015-01-01

    Purpose: To present our single institution experience on catching errors with trajectory log file analysis. The reported causes of failures, probability of occurrences (O), severity of effects (S), and the probability of the failures to be undetected (D) could be added to guidelines of FMEA analysis. Methods: From March 2013 to March 2014, 19569 patient treatment fields/arcs were analyzed. This work includes checking all 131 treatment delivery parameters for all patients, all treatment sites and all treatment delivery fractions. TrueBeam trajectory log files for all treatment field types as well as all imaging types were accessed, read in every 20ms, and every control point (total of 37 million parameters) compared to the physician approved plan in the planning system. Results: Couch angle outlier occurrence: N= 327, range = −1.7 −1.2 deg; gantry angle outlier occurrence: N =59, range = 0.09 – 5.61 deg, collimator angle outlier occurrence: N = 13, range = −0.2 – 0.2 deg. VMAT cases have slightly larger variations in mechanical parameters. MLC: 3D single control point fields have a maximum deviation of 0.04 mm, 39 step and shoot IMRT cases have MLC −0.3 – 0.5 mm deviations, all (1286) VMAT cases have −0.9 – 0.7 mm deviations. Two possible serious errors were found: 1) A 4 cm isocenter shift for the PA beam of an AP-PA pair, under-dosing a portion of PTV by 25%. 2) Delivery with MLC leaves abutted behind the jaws as opposed to the midline as planned, leading to a under-dosing of a small volume of the PTV by 25%, by just the boost plan. Due to their error origin, neither of these errors could have been detected by pre-treatment verification. Conclusion: Performing Trajectory Log file analysis could catch typically undetected errors to avoid potentially adverse incidents

  7. SU-E-T-158: Catching Errors with Trajectory Log Files

    Energy Technology Data Exchange (ETDEWEB)

    Wijesooriya, K; Seitter, K; Desai, V; Dharia, I; Read, P; Larner, J [University of Virginia Health Systems, Charlottesville, VA (United States)

    2015-06-15

    Purpose: To present our single institution experience on catching errors with trajectory log file analysis. The reported causes of failures, probability of occurrences (O), severity of effects (S), and the probability of the failures to be undetected (D) could be added to guidelines of FMEA analysis. Methods: From March 2013 to March 2014, 19569 patient treatment fields/arcs were analyzed. This work includes checking all 131 treatment delivery parameters for all patients, all treatment sites and all treatment delivery fractions. TrueBeam trajectory log files for all treatment field types as well as all imaging types were accessed, read in every 20ms, and every control point (total of 37 million parameters) compared to the physician approved plan in the planning system. Results: Couch angle outlier occurrence: N= 327, range = −1.7 −1.2 deg; gantry angle outlier occurrence: N =59, range = 0.09 – 5.61 deg, collimator angle outlier occurrence: N = 13, range = −0.2 – 0.2 deg. VMAT cases have slightly larger variations in mechanical parameters. MLC: 3D single control point fields have a maximum deviation of 0.04 mm, 39 step and shoot IMRT cases have MLC −0.3 – 0.5 mm deviations, all (1286) VMAT cases have −0.9 – 0.7 mm deviations. Two possible serious errors were found: 1) A 4 cm isocenter shift for the PA beam of an AP-PA pair, under-dosing a portion of PTV by 25%. 2) Delivery with MLC leaves abutted behind the jaws as opposed to the midline as planned, leading to a under-dosing of a small volume of the PTV by 25%, by just the boost plan. Due to their error origin, neither of these errors could have been detected by pre-treatment verification. Conclusion: Performing Trajectory Log file analysis could catch typically undetected errors to avoid potentially adverse incidents.

  8. Spatial and temporal consumption dynamics of trout in catch-and-release areas in Arkansas tailwaters

    Science.gov (United States)

    Flinders, John M.; Magoulick, Daniel D.

    2017-01-01

    Restrictive angling regulations in tailwater trout fisheries may be unsuccessful if food availability limits energy for fish to grow. We examined spatial and temporal variation in energy intake and growth in populations of Brown Trout Salmo trutta and Rainbow Trout Oncorhynchus mykiss within three catch-and-release (C-R) areas in Arkansas tailwaters to evaluate food availability compared with consumption. Based on bioenergetic simulations, Rainbow Trout fed at submaintenance levels in both size-classes (≤400 mm TL, >400 mm TL) throughout most seasons. A particular bottleneck in food availability occurred in the winter for Rainbow Trout when the daily ration was substantially below the minimum required for maintenance, despite reduced metabolic costs associated with lower water temperatures. Rainbow Trout growth rates followed a similar pattern to consumption with negative growth rates during the winter periods. All three size-classes (400 mm TL) of Brown Trout experienced high growth rates and limited temporal bottlenecks in food availability. We observed higher mean densities for Rainbow Trout (47–342 fish/ha) than for Brown Trout (3–84 fish/ha) in all C-R areas. Lower densities of Brown Trout coupled with an ontogenetic shift towards piscivory may have allowed for higher growth rates and sufficient consumption rates to meet energetic demands. Brown Trout at current densities were more effective in maintaining adequate growth rates and larger sizes in C-R areas than were Rainbow Trout. Bioenergetic simulations suggest that reducing stocking levels of Rainbow Trout in the tailwaters may be necessary in order to achieve increased catch rates of larger trout in the C-R areas.

  9. Effect of chemical ratios of a microbial-based feeding attractant on trap catch of spotted wing drosophila (Diptera: Drosophilidae)

    Science.gov (United States)

    Drosophila suzukii Matsumura (SWD) can be trapped with a feeding attractant based on wine and vinegar volatiles and consisting of acetic acid, ethanol, acetoin and methionol. Using that 4-component blend, we found that the catch of SWD increased with increases in the release rate of acetoin (from 0...

  10. Effects of contrasting catch crops on nitrogen availability and nitrous oxide emissions in an organic cropping system

    DEFF Research Database (Denmark)

    Li, Xiaoxi; Petersen, Søren O; Sørensen, Peter

    2015-01-01

    Legume-based catch crops (LBCCs) may act as an important source of nitrogen (N) in organic crop rotations because of biological N fixation. However, the potential risk of high nitrous oxide (N2O) emissions needs to be taken into account when including LBCCs in crop rotations. Here, we report...

  11. 'Catching up': The significance of occupational communities for the delivery of high quality home care by community nurses.

    Science.gov (United States)

    Adams, Mary; Robert, Glenn; Maben, Jill

    2013-07-01

    This article examines the importance of some informal work practices among community nurses during a period of significant organizational change. Ethnographic fieldwork in two purposively selected adult community nursing services in England comprised 79 hours of observation of routine practice, 21 interviews with staff and 23 interviews with patients. We identified the informal work practice of 'catching up', informal work conversations between immediate colleagues, as an important but often invisible aspect of satisfying work relationships and of the relational care of patients. Drawing on anthropological literatures on 'communities of practice' the article examines two central issues concerning the practices of 'catching up': (1) how informal learning processes shape community nursing work; (2) how this informal learning is shaped both in relation to the ideals of community nursing work and the wider political and organizational contexts of community nursing practice. Our findings highlight the distinctive value of informal workplace 'catch ups' for nurses to manage the inherent challenges of good home care for patients and to develop a shared ethic of care and professional identity. Our findings also indicate the decline of 'catching up' between nurses along with diminishing time and opportunity for staff to care holistically for patients in present service climates.

  12. 77 FR 46014 - Main Hawaiian Islands Deep 7 Bottomfish Annual Catch Limits and Accountability Measures for 2012-13

    Science.gov (United States)

    2012-08-02

    ... DEPARTMENT OF COMMERCE National Oceanic and Atmospheric Administration 50 CFR Part 665 [Docket No. 120628195-2276-01] RIN 0648-XC089 Main Hawaiian Islands Deep 7 Bottomfish Annual Catch Limits and Accountability Measures for 2012-13 AGENCY: National Marine Fisheries Service (NMFS), National Oceanic and...

  13. 76 FR 44155 - Pacific Halibut Fisheries; Catch Sharing Plan for Guided Sport and Commercial Fisheries in Alaska

    Science.gov (United States)

    2011-07-22

    ... Halibut Fisheries II. History of Management in the Guided Sport Halibut Fisheries III. Proposed Catch... consistency and uniformity in halibut sport fishing regulations in all regulatory areas. At that time, the... (649.5 mt). During that time period, guided sport harvests were approximately 1,750,000 lbs (793.8 mt...

  14. 77 FR 42192 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Comprehensive Annual Catch Limit...

    Science.gov (United States)

    2012-07-18

    ... statement, a regulatory flexibility analysis, and a regulatory impact review, may be obtained from the... is the acceptable biological catch (ABC). Based on the most recent stock assessment completed in 2008, the SSC recommended a greater amberjack stock ABC of 1,968,000 lb (892,670 kg), round weight...

  15. Nitrogen leaching: A crop rotation perspective on the effect of N surplus, field management and use of catch crops

    DEFF Research Database (Denmark)

    De Notaris, Chiara; Rasmussen, Jim; Sørensen, Peter

    2018-01-01

    Components of the field nitrogen (N) balance (input and surplus) are often used to predict nitrate leaching from agricultural lands. However, management factors, such as use of catch crops, greatly affect the actual loss and are a key to reduce N leaching. The present study is based on the 4th cy...

  16. Positive Catch & Economic Benefits of Periodic Octopus Fishery Closures: Do Effective, Narrowly Targeted Actions ‘Catalyze’ Broader Management?

    Science.gov (United States)

    Oliver, Thomas A.; Oleson, Kirsten L. L.; Ratsimbazafy, Hajanaina; Raberinary, Daniel; Benbow, Sophie; Harris, Alasdair

    2015-01-01

    Overview Eight years of octopus fishery records from southwest Madagascar reveal significant positive impacts from 36 periodic closures on: (a) fishery catches and (b) village fishery income, such that (c) economic benefits from increased landings outweigh costs of foregone catch. Closures covered ~20% of a village’s fished area and lasted 2-7 months. Fishery Catches from Each Closed Site Octopus landings and catch per unit effort (CPUE) significantly increased in the 30 days following a closure’s reopening, relative to the 30 days before a closure (landings: +718%, poctopus fishery income doubled in the 30 days after a closure, relative to 30 days before (+132%, p<0.001, n = 28). Control villages not implementing a closure showed no increase in income after “no ban” closures and modest increases after “ban” closures. Villages did not show a significant decline in income during closure events. Net Economic Benefits from Each Closed Site Landings in closure sites generated more revenue than simulated landings assuming continued open-access fishing at that site (27/36 show positive net earnings; mean +$305/closure; mean +57.7% monthly). Benefits accrued faster than local fishers’ time preferences during 17-27 of the 36 closures. High reported rates of illegal fishing during closures correlated with poor economic performance. Broader Co-Management We discuss the implications of our findings for broader co-management arrangements, particularly for catalyzing more comprehensive management. PMID:26083862

  17. Effect of bottom type on catch rates of North Sea cod (Gadus morhua) in surveys with commercial fishing vessels

    DEFF Research Database (Denmark)

    Wieland, Kai; Pedersen, Eva Maria; Olesen, Hans Jakob

    2009-01-01

    were substantially higher on gravel or stone bottom and at ship wrecks than on sand bottom. The difference in the catch rates between the two bottom categories at paired stations within a short distance was highly significant for all the three fishing methods. Similarly, average CPUE for most surveys...

  18. The effect of early catch-up growth on health and well-being in young adults

    NARCIS (Netherlands)

    Dommelen, P. van; Pal-de Bruin, S.M. van der; Bennebroek Gravenhorst, J.; Walther, F.J.; Wit, J.M.; Pal, K.M. van der

    2014-01-01

    Aim: To study the effect of catch-up growth in the 1st year on cognition, health-related quality of life (HRQoL), problem behavior and growth in young adults. Methods: We included individuals without severe complications and born small for gestational age (SGA; n = 228 for weight, n = 203 for

  19. Getting hold of approaching objects : In search of a common control of hand-closure initiation in catching and grasping

    NARCIS (Netherlands)

    van de Kamp, Cornelis; Bongers, Raoul M.; Zaal, Frank T. J. M.

    Both in the catching and grasping component of prehension, the hand opens and closes before hand-object contact is made. The initiation of hand closure has to be coordinated with the time course of the decrease of the distance between the hand and the target object, i.e., with the reaching component

  20. Catch up patterns in newly industrializing countries : an international comparison of manufacturing productivity in Taiwan, 1961-1993

    NARCIS (Netherlands)

    Timmer, Marcel P.

    1998-01-01

    Taiwan has undergone a process of swift industrialization after 1948. Rapid accumulation of physical and human capital enabled Taiwan to exploit new technologies and products, resulting in rapid catch up in labour productivity relative to more advanced economies. Using the industry-of-origin

  1. Role of University-Industry-Government Linkages in the Innovation Processes of a Small Catching-Up Economy

    Science.gov (United States)

    Varblane, Urmas; Mets, Tonis; Ukrainski, Kadri

    2008-01-01

    During the transformation process from a command economy, the extraordinary statist university-industry-government (UIG) linkages model was replaced by an extreme version of laissez-faire relationships. A more modern interaction-based UIG model could be implemented only by changing the whole national innovation system of catching-up economies. The…

  2. How to catch all those mutations--the report of the third Human Variome Project Meeting, UNESCO Paris, May 2010

    DEFF Research Database (Denmark)

    Kohonen-Corish, Maija R J; Al-Aama, Jumana Y; Auerbach, Arleen D

    2010-01-01

    . The HVP has drawn together disparate groups, by country, gene of interest, and expertise, who are working for the common good with the shared goal of pushing the boundaries of the human variome and collaborating to avoid unnecessary duplication. The meeting addressed the 12 key areas that form the current...

  3. Promoting teacher adoption of physical activity breaks in the classroom: findings of the Central Texas CATCH Middle School Project.

    Science.gov (United States)

    Delk, Joanne; Springer, Andrew E; Kelder, Steven H; Grayless, Megan

    2014-11-01

    Research suggests that physical activity breaks (ABs) during class increase students' physical activity levels and provide an academic benefit. This study evaluates a 3-year intervention aimed at encouraging teacher AB use. Thirty central Texas middle schools were assigned to 1 of 3 conditions: training-only (Basic), training plus facilitator support (Basic Plus), and training/facilitator support and a social marketing campaign (Basic Plus SM). Teachers completed surveys at end of years 2 (N = 1039) and 3 (N = 831) to assess exposure to program, self-efficacy, and frequency of AB use. At end of year 3, teachers in facilitator-supported conditions reported increased exposure, self-efficacy, and use compared to Basic condition. Only 43.2% of teachers in the Basic condition reported receiving training in ABs compared to 84.2% and 90.6% in the Basic Plus and Basic Plus SM conditions, respectively. Additionally, a greater percentage of teachers in the facilitator-support conditions reported conducting ABs weekly (Basic = 23.3%, Basic Plus = 34.4%, Basic Plus SM = 38.7%, at year 3; p < .001). Despite perceived barriers, including fear that ABs will detract from instructional time, the intervention was successful in having a core group of teachers implement them weekly. More research is needed to increase the percentage of teachers implementing ABs regularly. © 2014, American School Health Association.

  4. Investigation of miRNA Biology by Bioinformatic Tools and Impact of miRNAs in Colorectal Cancer: Regulatory Relationship of c-Myc and p53 with miRNAs

    Directory of Open Access Journals (Sweden)

    Yaguang Xi

    2007-01-01

    Full Text Available MicroRNAs (miRNAs are a class of small non-coding RNAs that mediate gene expression at the posttranscriptional and translational levels and have been demonstrated to be involved in diverse biological functions. Mounting evidence in recent years has shown that miRNAs play key roles in tumorigenesis due to abnormal expression of and mutations in miRNAs. High throughput miRNA expression profiling of several major tumor types has identified miRNAs associated with clinical diagnosis and prognosis of cancer treatment. Previously our group has discovered a novel regulatory relationship between tumor suppressor gene p53 with miRNAs expression and a number of miRNA promoters contain putative p53 binding sites. In addition, others have reported that c-myc can mediate a large number of miRNAs expression. In this review, we will emphasize algorithms to identify mRNA targets of miRNAs and the roles of miRNAs in colorectal cancer. In particular, we will discuss a novel regulatory relationship of miRNAs with tumor suppressor p53 and c-myc. miRNAs are becoming promising novel targets and biomarkers for future cancer therapeutic development and clinical molecular diagnosis.

  5. Vitamin K2 and cotylenin A synergistically induce monocytic differentiation and growth arrest along with the suppression of c-MYC expression and induction of cyclin G2 expression in human leukemia HL-60 cells.

    Science.gov (United States)

    Maniwa, Yasuhisa; Kasukabe, Takashi; Kumakura, Shunichi

    2015-08-01

    Although all-trans retinoic acid (ATRA) is a standard and effective drug used for differentiation therapy in acute promyelocytic leukemia, ATRA-resistant leukemia cells ultimately emerge during this treatment. Therefore, the development of new drugs or effective combination therapy is urgently needed. We demonstrate that the combined treatment of vitamin K2 and cotylenin A synergistically induced monocytic differentiation in HL-60 cells. This combined treatment also synergistically induced NBT-reducing activity and non-specific esterase-positive cells as well as morphological changes to monocyte/macrophage-like cells. Vitamin K2 and cotylenin A cooperatively inhibited the proliferation of HL-60 cells in short-term and long-term cultures. This treatment also induced growth arrest at the G1 phase. Although 5 µg/ml cotylenin A or 5 µM vitamin K2 alone reduced c-MYC gene expression in HL-60 cells to approximately 45% or 80% that of control cells, respectively, the combined treatment almost completely suppressed c-MYC gene expression. We also demonstrated that the combined treatment of vitamin K2 and cotylenin A synergistically induced the expression of cyclin G2, which had a positive effect on the promotion and maintenance of cell cycle arrest. These results suggest that the combination of vitamin K2 and cotylenin A has therapeutic value in the treatment of acute myeloid leukemia.

  6. Loss of connective tissue growth factor as an unfavorable prognosis factor activates miR-18b by PI3K/AKT/C-Jun and C-Myc and promotes cell growth in nasopharyngeal carcinoma.

    Science.gov (United States)

    Yu, X; Zhen, Y; Yang, H; Wang, H; Zhou, Y; Wang, E; Marincola, F M; Mai, C; Chen, Y; Wei, H; Song, Y; Lyu, X; Ye, Y; Cai, L; Wu, Q; Zhao, M; Hua, S; Fu, Q; Zhang, Y; Yao, K; Liu, Z; Li, X; Fang, W

    2013-05-16

    Connective tissue growth factor (CTGF) has different roles in different types of cancer. However, the involvement and molecular basis of CTGF in tumor progression and prognosis of human nasopharyngeal carcinoma (NPC) have almost never been reported. In this study, we observed that downregulated CTGF expression was significantly associated with NPC progression and poor prognosis. Knockdown of CTGF markedly elevated the ability of cell proliferation in vivo and in vitro. Subsequently, we discovered that the reduction of CTGF increased the expression of miR-18b, an oncomir-promoting cell proliferation. Further, we discovered that attenuated CTGF-mediated upregulation of miR-18b was dependent on the increased binding of transcription factors Jun proto-oncogene (C-Jun) and v-Myc myelocytomatosis viral oncogene homolog (C-Myc) to miR-18b promoter region via phosphoinositide 3-kinase (PI3K)/AKT pathway. Finally, we further found that miR-18b directly suppressed the expression of CTGF in NPC. In clinical fresh specimens, miR-18b was widely overexpressed and inversely correlated with CTGF expression in NPC. Our studies are the first to demonstrate that reduced CTGF as an unfavorable prognosis factor mediates the activation of miR-18b, an oncomir directly suppresses CTGF expression, by PI3K/AKT/C-Jun and C-Myc and promotes cell growth of NPC.

  7. Spatio-temporal declines in Philippine fisheries and its implications to coastal municipal fishers’ catch and income

    Directory of Open Access Journals (Sweden)

    Jonathan A Anticamara

    2016-03-01

    Full Text Available The problem of overexploitation in global fisheries is well-recognized. However, published assessment of fisheries spatio-temporal trends at the national scale is lacking for many high biodiversity developing countries, which is problematic since fisheries management is often implemented at the local or national levels. Here, we present the long-term spatio-temporal trends of Philippine fisheries production based on the landed national fish catch data (1980-2012 and fishers’ interviews. We found that the total Philippine fish catch volume (Metric Tons MT of most capture fisheries throughout the country has either stagnated or declined over the last three decades. The decline is even more prominent when evaluating fisheries trends at the provincial level, suggesting spatial serial depletion of the country’s fisheries. In contrast, the total Philippine fish catch value (US Dollars US$ or Philippine Pesos PHP has continued to increase over time, despite the declining fish catch volume. However, local municipal fishers are experiencing both low fish catch and income, contributing to observable poverty in many coastal communities in the Philippines. The various stakeholders of Philippine fisheries need to recognize the depleted state of Philippine fisheries, and learn from various experiences of collapsed and recovered fisheries from around the world, in order to recover the Philippines’ capture fisheries. Lessons from the literature on collapsed fisheries offer the following options for recovery: (1 regulate or reduce fisheries exploitation and other human activities impacting the fisheries to allow fisheries to rebuild or recover, (2 enforce effective networks of marine reserves, (3 engage fishers, consumers, and other stakeholders in fisheries management, (4 improve fisheries science, monitoring, and management capacities, and (5 provide alternative livelihood, skills, and improved education to fishers and their families.

  8. Dynamics of Bacillus thuringiensis var. israelensis and Lysinibacillus sphaericus spores in urban catch basins after simultaneous application against mosquito larvae.

    Directory of Open Access Journals (Sweden)

    Valeria Guidi

    Full Text Available Bacillus thuringiensis var. israelensis (Bti and Lysinibacillus sphaericus (Lsph are extensively used in mosquito control programs. These biocides are the active ingredients of a commercial larvicide. Quantitative data on the fate of both Bti and Lsph applied together for the control of mosquitoes in urban drainage structures such as catch basins are lacking. We evaluated the dynamics and persistence of Bti and Lsph spores released through their concomitant application in urban catch basins in southern Switzerland. Detection and quantification of spores over time in water and sludge samples from catch basins were carried out using quantitative real-time PCR targeting both cry4A and cry4B toxin genes for Bti and the binA gene for Lsph. After treatment, Bti and Lsph spores attained concentrations of 3.76 (± 0.08 and 4.13 (± 0.09 log ml(-1 in water, then decreased progressively over time, reaching baseline values. For both Bti and Lsph, spore levels in the order of 10(5 g(-1 were observed in the bottom sludge two days after the treatment and remained constant for the whole test period (275 days. Indigenous Lsph strains were isolated from previously untreated catch basins. A selection of those was genotyped using pulsed field gel electrophoresis of SmaI-digested chromosomal DNA, revealing that a subset of isolates were members of the clonal population of strain 2362. No safety issues related to the use of this biopesticide in the environment have been observed during this study, because no significant increase in the number of spores was seen during the long observation period. The isolation of native Lysinibacillus sphaericus strains belonging to the same clonal population as strain 2362 from catch basins never treated with Lsph-based products indicates that the use of a combination of Bti and Lsph for the control of mosquitoes does not introduce non-indigenous microorganisms in this area.

  9. Functional design criteria radioactive liquid waste line replacement, Project W-087. Revision 3

    International Nuclear Information System (INIS)

    McVey, C.B.

    1994-01-01

    This document provides the functional design criteria for the 222-S Laboratory radioactive waste drain piping and transfer pipeline replacement. The project will replace the radioactive waste drain piping from the hot cells in 222-S to the 219-S Waste Handling Facility and provide a new waste transfer route from 219-S to the 244-S Catch Station in Tank Farms

  10. The use of computerized video time lapse to study cell death in rat embryo cells transfected with c-ha-ras or c-myc

    International Nuclear Information System (INIS)

    Forrester, H.B.; Vidair, C.A.; Dewey, W.C.; Ling, C.C.

    1998-01-01

    Full text: Individual rat embryo fibroblasts that had been transfected with the c-myc (REC:myc) or c-Ha ras (REC:ras) oncogene were followed after irradiation using a computer video time lapse (CVTL) system in order to quantify the lethal events that resulted in loss of clonogenic survival after irradiation. By followed the cells for 2 to 3 generations before irradiation we were able to determine where they were in the cell cycle at the time of irradiation for cell cycle analysis. After irradiation, the individual cells and their progeny were followed in multiple fields for 5-6 days Then, pedigrees for individual irradiated cells were determined by noting the times of divisions fusions, and cell death. After X-irradiation, the clonogenic survival values for these two cell lines are similar. However, by using computerized video time lapse (CVTL) to follow individual cells we found that the loss of clonogenic survival was due to two different processes, cell death and a senescent-like process. The loss of clonogenic survival of x-irradiated (9.5 and 4 Gy) REC:myc cells was attributed almost entirely to the cells dying by apoptosis (∼99 and 90%). In contrast, approximately 60% of the x-irradiated (9.5 Gy) non-clonogenic REC:ras cells died by apoptosis (with a very small amount of necrosis), and the other 40% underwent a senescent-type process in which some of the cells and their progeny stopped dividing but remained as viable cells throughout 140 hours of observation. Both processes usually occurred after the cells had divided and continued to occur in the cells' progeny for up to five divisions after irradiation. The mode of cell death in the progeny of a non-clonogenic cell can be determined only by using CVTL and can not be determined by conventional clonogenic survival experiments. Also, only by following the individual cells and their progeny can the true amount of apoptosis be determined. The cumulative percentage of apoptosis scored in whole populations

  11. Estimation of total catch of silver kob Argyrosomus inodorus by recreational shore-anglers in Namibia using a roving-roving creel survey

    DEFF Research Database (Denmark)

    Kirchner, C.H.; Beyer, Jan

    1999-01-01

    , using data taken during a survey from 1 October 1995 to 30 September 1996. Two different methods of estimating daily catch were tested by sampling the same population of anglers using a complete and an incomplete survey. The mean rate estimator, calculated by the ratio of the means with progressive......A statistical sampling method is described to estimate the annual catch of silver kob Agryrosomus indorus by recreational shore-anglers in Namibia. The method is based on the theory of progressive counts and on-site roving interviews of anglers, with catch counts and measurements at interception...

  12. The feasibility and effectiveness of Catch It, an innovative CBT smartphone app.

    Science.gov (United States)

    Kinderman, Peter; Hagan, Paul; King, Sophie; Bowman, James; Chahal, Jasprit; Gan, Li; McKnight, Rebecca; Waldon, Charlotte; Smith, Matthew; Gilbertson, John; Tai, Sara

    2016-05-01

    The widespread use of smartphones makes effective therapies such as cognitive-behavioural therapy (CBT) potentially accessible to large numbers of people. This paper reports the usage data of the first trial of Catch It, a new CBT smartphone app. Uptake and usage rates, fidelity of user responses to CBT principles, and impact on reported negative and positive moods were assessed. A relatively modest proportion of people chose to download the app. Once used, the app tended to be used more than once, and 84% of the user-generated content was consistent with the basic concepts of CBT. There were statistically significant reductions in negative mood intensity and increases in positive mood intensity. Smartphone apps have potential beneficial effects in mental health through the application of basic CBT principles. More research with randomised controlled trial designs should be conducted. None. © The Royal College of Psychiatrists 2016. This is an open access article distributed under the terms of the Creative Commons Non-Commercial, No Derivatives (CC BY-NC-ND) licence.

  13. CATCH COMPOSITION AND SOME BIOLOGICAL ASPECTS OF SHARKS IN WESTERN SUMATERA WATERS OF INDONESIA

    Directory of Open Access Journals (Sweden)

    Dharmadi Dharmadi

    2017-01-01

    Full Text Available This study was conducted in western Sumatera and since October 2013 to June 2014. The sampling locations in Banda Aceh and Sibolga-North Sumatera which were the largest base of fisheries in western Sumatera region. Shark landing recorded by enumerators was used  as sampling data daily . This research aim to describ sex ratio, size composition, catch composition of sharks, and length at first maturity. In Banda Aceh, the sharks as target fish collected by  sorting the bycatch from tuna longlines and tuna handlines. In Sibolga, sharks  is bycatch from fish net, bottom gillnet and purse seine. Overall, there were 20 species of shark caught in west Indian Ocean and landed at those fish landing sites, dominated by Spot tail shark (23% and Silky shark (13%, whereas Hammerhead shark contributed about 10% and  Oceanic whitetip shark was only less than 1%. Almost of Spot tail shark, Silky shark, and Scalloped hammerhead that caught in that area were  immature, while for the almost part of Tiger shark and Pelagic thresher were  matured. The sex ratios for Spot tail shark, Silky shark, Tiger shark, Pelagic thresher, and Scalloped hammerhead caught and landed at Lampulo and Sibolga fish landing sites were not balance. The length at first maturity for Spot tail shark was Lm=87,1 cm and Lm = 213,2 cm total length for Tiger shark.

  14. Beta Palmitate Improves Bone Length and Quality during Catch-Up Growth in Young Rats

    Directory of Open Access Journals (Sweden)

    Meytal Bar-Maisels

    2017-07-01

    Full Text Available Palmitic acid (PA is the most abundant saturated fatty acid in human milk, where it is heavily concentrated in the sn-2-position (termed beta palmitate, BPA and as such is conserved in all women, regardless of their diet or ethnicity, indicating its physiological and metabolic importance. We hypothesized that BPA improves the efficiency of nutrition-induced catch up growth as compared to sn-1,3 PA, which is present in vegetable oil. Pre-pubertal male rats were subjected to a 17 days food restriction followed by re-feeding for nine days with 1,3 PA or BPA-containing diets. We measured bone length, epiphyseal growth plate height (EGP, histology, bone quality (micro-CT and 3-point bending assay, and gene expression (Affymetrix. The BPA-containing diet improved most growth parameters: humeri length and EGP height were greater in the BPA-fed animals. Further analysis of the EGP revealed that the hypertrophic zone was significantly higher in the BPA group. In addition, Affymetrix analysis revealed that the diet affected the expression of several genes in the liver and EGP. Despite the very subtle difference between the diets and the short re-feeding period, we found a small but significant improvement in most growth parameters in the BPA-fed rats. This pre-clinical study may have important implications, especially for children with growth disorders and children with special nutritional needs.

  15. Incidental catch of marine turtles by the artisanal fisheries on Santa Catarina Island, SC, Brazil

    Directory of Open Access Journals (Sweden)

    Natalia Hanazaki

    2006-12-01

    Full Text Available The five species of sea turtles, which inhabit the Brazilian shore, have a wordwide distribution and are threatened with extinction. The south of Brazil is characterized as a feeding and breeding area of at least three of these species. The presence of turtles close to the shore and their incidental catch are occurrences reported by artisan fishermen of the Island of Santa Catarina in this work. The study was based on a questionnaire, applied to fishermen and fishfarmers at selected spots on the island. Scientific and popular names were matched using illustrations. The spots with the highest number of events and captures were close to the islands, rocky coasts and stony grounds, i.e. areas related with the foraging habits of the most abundant species, Chelonia mydas. The place, depth and size of the net are characteristics, which influence the capture. The time the net remains in the water is a factor which is fundamental to the survival of the captured turtles. The flesh of the turtle is used as an occasional feeding resource. Capacitation programs and training applied to the communities involved are recommended.

  16. Catching a Wave: The Hypnosis-Sensitive Transference-Based Treatment of Dissociative Identity Disorder (DID).

    Science.gov (United States)

    Brenner, Ira

    2018-01-01

    In this article, I will describe the way in which I work with enactment-prone dissociative patients in the transference. This approach requires an appreciation of the phenomena of hypnosis and the auto-hypnotic aspects of some forms of dissociation. Essentially, I learn from the patient and my interactions with the patient how hypnotic phenomena and auto-hypnotic defenses manifest themselves in the therapeutic relationship in order both to understand them and ultimately to bring them under conscious control. Because of the fluidity and turbulence of these states, I use the analogy of catching a wave, in which timing and balance are essential, albeit elusive factors in effecting a successful treatment. The importance of having experience with many patients, attending conferences, seeking supervision, and undergoing one's own therapy will be also discussed as important prerequisites for the clinician endeavoring to utilize this type of approach. This preparation, this quest for such a "balance," is modeled after the so-called tripartite model of training employed in psychoanalytic training institutes. I will offer clinical material to illustrate this approach, which I have described as "psychoactive psychotherapy." In such treatments, the clinician may be taken by surprise and is likely to be thrown "off balance" from time to time. The mutually shared understanding of such moments is essential to regaining clinical balance in the therapeutic setting, and can lead to if not create important turning points in the treatment process.

  17. Catching moving targets: cancer stem cell hierarchies, therapy-resistance & considerations for clinical intervention.

    LENUS (Irish Health Repository)

    Gasch, Claudia

    2017-01-01

    It is widely believed that targeting the tumour-initiating cancer stem cell (CSC) component of malignancy has great therapeutic potential, particularly in therapy-resistant disease. However, despite concerted efforts, CSC-targeting strategies have not been efficiently translated to the clinic. This is partly due to our incomplete understanding of the mechanisms underlying CSC therapy-resistance. In particular, the relationship between therapy-resistance and the organisation of CSCs as Stem-Progenitor-Differentiated cell hierarchies has not been widely studied. In this review we argue that modern clinical strategies should appreciate that the CSC hierarchy is a dynamic target that contains sensitive and resistant components and expresses a collection of therapy-resisting mechanisms. We propose that the CSC hierarchy at primary presentation changes in response to clinical intervention, resulting in a recurrent malignancy that should be targeted differently. As such, addressing the hierarchical organisation of CSCs into our bench-side theory should expedite translation of CSC-targeting to bed-side practice. In conclusion, we discuss strategies through which we can catch these moving clinical targets to specifically compromise therapy-resistant disease.

  18. Trawl catch composition during different fishing intensity periods in two Mediterranean demersal fishing grounds

    Directory of Open Access Journals (Sweden)

    Pilar Sánchez

    2007-12-01

    Full Text Available The study was carried out in two fishing grounds on the Mediterranean continental shelf: one in the Adriatic Sea and one in the Catalan Sea. Samplings on board otter trawlers were performed from November 2002 to December 2003 in the Catalan Sea and from February 2003 to January 2004 in the Adriatic Sea. The Adriatic fishing ground was affected by high fishing intensity from January to June, while the Catalan area was highly exploited from September to February. Fishing activity in the Adriatic area was closed for 45 days, and 62 days in the Catalan area; both periods were in summer. Totals of 92 and 88 species were collected in the Adriatic and Catalan fishing grounds respectively. The species composition of the retained and discarded fractions showed close agreement between the two areas. Mullus barbatus showed very low discard rates in both areas, as well as Loligo vulgaris in the Catalan Sea and Merluccius merluccius in the Adriatic Sea. In both fishing grounds Squilla mantis showed high catch rates with low discards, except in March in the Catalan area. In the Adriatic Sea Liocarcinus depurator was characterized by large discard fractions. In both fishing grounds the retained fraction was slightly higher in the high fishing intensity periods than in the low intensity ones (Adriatic Sea: 0.613 vs 0.524; Catalan Sea: 0.597 vs 0.539, even though the Kruskall Wallis test revealed significant differences (p

  19. A prototype splitter apparatus for dividing large catches of small fish

    Science.gov (United States)

    Stapanian, Martin A.; Edwards, William H.

    2012-01-01

    Due to financial and time constraints, it is often necessary in fisheries studies to divide large samples of fish and estimate total catch from the subsample. The subsampling procedure may involve potential human biases or may be difficult to perform in rough conditions. We present a prototype gravity-fed splitter apparatus for dividing large samples of small fish (30–100 mm TL). The apparatus features a tapered hopper with a sliding and removable shutter. The apparatus provides a comparatively stable platform for objectively obtaining subsamples, and it can be modified to accommodate different sizes of fish and different sample volumes. The apparatus is easy to build, inexpensive, and convenient to use in the field. To illustrate the performance of the apparatus, we divided three samples (total N = 2,000 fish) composed of four fish species. Our results indicated no significant bias in estimating either the number or proportion of each species from the subsample. Use of this apparatus or a similar apparatus can help to standardize subsampling procedures in large surveys of fish. The apparatus could be used for other applications that require dividing a large amount of material into one or more smaller subsamples.

  20. Effect that Smell Presentation Has on an Individual in Regards to Eye Catching and Memory

    Science.gov (United States)

    Tomono, Akira; Kanda, Koyori; Otake, Syunya

    If a person's eyes are greater attracted to the target objects by matching the smell to an important scene of a movie or commercial image, the value of the image contents will rise. In this paper, we attempt to describe the image system that can also present smells, and the reason behind the improvement, from gaze point analysis, of the presence of smell when it is matched to the image. The relationship between the eye catching property and the position of the sight object was examined using the image with the scene where someone eats three kinds of fruits. These objects were gazed at for a long time once releasing their smells. When the smell was not released, the gaze moved actively to try and receive a lot of information from the entire screen. On the other hand, when the smell was inserted, the subject was interested in the object and there was a tendency for their gaze to stay within the narrow area surrounding the image. Moreover, we investigated the effect on the memory by putting the smell on the flowers in the virtual flower shop using immersive virtual reality system (HoloStageTM). It was memorized more easily compared with a scentless case. It seems that the viewer obtains the information actively by reacting to its smell.

  1. Catch-up growth does not associate with cognitive development in Indian school-age children.

    Science.gov (United States)

    Sokolovic, N; Selvam, S; Srinivasan, K; Thankachan, P; Kurpad, A V; Thomas, T

    2014-01-01

    Stunting is significantly associated with lifetime morbidity and poorer cognitive outcomes in children. Although several studies have examined the relationship between stunting, catch-up growth and cognitive performance in young populations, this relationship has not yet been explored in school-aged children. In this study, we used data from three different nutritional intervention studies conducted over a 4-year period on school-age children in Bangalore, India to assess these relationships. A battery of cognitive tests was conducted before each intervention to determine whether stunting status at baseline was related to cognitive performance across four separate domains, and repeated after a 6-month period to assess whether changes to stunting status is related to cognitive advancement. Results of independent t-tests showed that while stunted children had significantly poorer performance on short-term memory, retrieval ability and visuospatial ability tests (P=0.023, 0.026 and 0.028, respectively), there was no significant difference in the change in cognitive scores following nutritional interventions over a 6-month period between those who remained stunted and those who were no longer stunted (P>0.10). Evidently, stunting remains associated with cognitive ability in school-age children; however, the reversal of these effects in this age group may be quite difficult.

  2. The range of attraction for light traps catching Culicoides biting midges (Diptera: Ceratopogonidae)

    DEFF Research Database (Denmark)

    Kirkeby, Carsten; Græsbøll, Kaare; Stockmarr, Anders

    2013-01-01

    Background Culicoides are vectors of e.g. bluetongue virus and Schmallenberg virus in northern Europe. Light trapping is an important tool for detecting the presence and quantifying the abundance of vectors in the field. Until now, few studies have investigated the range of attraction of light tr...... light trap was estimated to be approximately 15.25 meters. The attraction towards light traps is different from the attraction to host animals and thus light trap catches may not represent the vector species and numbers attracted to hosts.......Background Culicoides are vectors of e.g. bluetongue virus and Schmallenberg virus in northern Europe. Light trapping is an important tool for detecting the presence and quantifying the abundance of vectors in the field. Until now, few studies have investigated the range of attraction of light...... traps. Methods Here we test a previously described mathematical model (Model I) and two novel models for the attraction of vectors to light traps (Model II and III). In Model I, Culicoides fly to the nearest trap from within a fixed range of attraction. In Model II Culicoides fly towards areas...

  3. Case study: Is the 'catch-all-plastics bin' useful in unlocking the hidden resource potential in the residual waste collection system?

    Science.gov (United States)

    Kranzinger, Lukas; Schopf, Kerstin; Pomberger, Roland; Punesch, Elisabeth

    2017-02-01

    Austria's performance in the collection of separated waste is adequate. However, the residual waste still contains substantial amounts of recyclable materials - for example, plastics, paper and board, glass and composite packaging. Plastics (lightweight packaging and similar non-packaging materials) are detected at an average mass content of 13% in residual waste. Despite this huge potential, only 3% of the total amount of residual waste (1,687,000 t y -1 ) is recycled. This implies that most of the recyclable materials contained in the residual waste are destined for thermal recovery and are lost for recycling. This pilot project, commissioned by the Land of Lower Austria, applied a holistic approach, unique in Europe, to the Lower Austrian waste management system. It aims to transfer excess quantities of plastic packaging and non-packaging recyclables from the residual waste system to the separately collected waste system by introducing a so-called 'catch-all-plastics bin'. A quantity flow model was constructed and the results showed a realistic increase in the amount of plastics collected of 33.9 wt%. This equals a calculated excess quantity of 19,638 t y -1 . The increased plastics collection resulted in a positive impact on the climate footprint (CO 2 equivalent) in line with the targets of EU Directive 94/62/EG (Circular Economy Package) and its Amendments. The new collection system involves only moderate additional costs.

  4. Material/historic Reality: Catching the Transformation. From a Case of Applied Research to the Trans-Disciplinary Approach to Preserve Architecture

    Science.gov (United States)

    Aveta, A.; Marino, B. G.; Amore, R.

    2017-05-01

    The present paper aims at dealing with some issues of knowledge of the architectural heritage. Given the increasing use of the innovative technologies in the field of the cultural heritage it is important to focus on their usefulness and potentialities in order to the conservation project management. The role of the new survey techniques and the accurate representations of the dimensional, structural and material consistency of the historic buildings and their context is mandatory and can influence the restoration choices. Starting from a recent applied research concerning a significant and symbolic monument of Naples, Castel Nuovo, the paper intends to highlight not only the importance of the integration of the specialist surveys, but also the role of the critical interpretation. The results of the different disciplines involved in the knowledge process have to be evaluated critically in view of the conservation of the tangible and intangible values. Furthermore, catching the complexity of architecture of the past depends on the capacity to maintain a close and constant contactwith the building physicality and also on a complex methodology which is inclusive of new interpretative instruments which could increase a virtuous hermeneutic circle.

  5. Utility Function and Optimum Consumption in the models with Habit Formation and Catching up with the Joneses

    OpenAIRE

    Naryshkin, Roman; Davison, Matt

    2009-01-01

    This paper analyzes popular time-nonseparable utility functions that describe "habit formation" consumer preferences comparing current consumption with the time averaged past consumption of the same individual and "catching up with the Joneses" (CuJ) models comparing individual consumption with a cross-sectional average consumption level. Few of these models give reasonable optimum consumption time series. We introduce theoretically justified utility specifications leading to a plausible cons...

  6. Correlation between catch data from bottom longlines and fish censures in the SW lagoon of New Caledonia

    OpenAIRE

    Kulbicki, Michel

    1988-01-01

    On a total of 363 bottoms longline sets in the SW lagoon of New Caledonia, 45 were surveyed using visual census. Abundance and biomass estimates were derived from these censuses. These estimates were highly correlated to catch per unit effort in numbers and weight. From these relationships were established contour maps of soft bottom fish abundance and biomass. The total biomass of longline catchable fish was estimated between 11800 and 25500 tons with an average of 17700 tons which represent...

  7. Factors influencing the technology upgrading and catch-up of Chinese wind turbine manufacturers: Technology acquisition mechanisms and government policies

    International Nuclear Information System (INIS)

    Qiu, Yueming; Ortolano, Leonard; David Wang, Yi

    2013-01-01

    This paper uses firm level data for the Chinese wind turbine manufacturing industry from 1998 to 2009 to quantify the effects of technology acquisition mechanisms – purchasing production licenses from foreign manufacturers, joint design with foreign design firms, joint-ventures and domestic R and D – on wind turbine manufacturers' technology levels (as measured by turbine size, in megawatts). It also examines the impacts of government policies on manufacturer technology levels. Technology upgrading (measured by increase of turbine size) and catch-up (measured by decrease in the distance to the world technology frontier in terms of turbine size) are used to measure advances in technology level. Results from econometric modeling studies indicate that firms' technology acquisition mechanisms and degree of business diversification are statistically significant factors in influencing technology upgrading. Similar results were found for the catch-up variable (i.e., distance to the world technology frontier). The influence of government policies is significant for technology upgrading but not catch-up. These and other modeling results are shown to have implications for both policymakers and wind turbine manufacturers. - Highlights: ► Technology acquired through joint design has the highest level. ► Technology acquired through purchasing production license has the lowest level. ► Technology acquired through domestic R and D has the level in between. ► A firm with related other businesses tends to have a higher level of technology. ► The influence of policies is significant for technology upgrade but not catch-up

  8. Hepatic mitochondrial energetics during catch-up fat with high-fat diets rich in lard or safflower oil.

    Science.gov (United States)

    Crescenzo, Raffaella; Bianco, Francesca; Falcone, Italia; Tsalouhidou, Sofia; Yepuri, Gayathri; Mougios, Vassilis; Dulloo, Abdul G; Liverini, Giovanna; Iossa, Susanna

    2012-09-01

    We have investigated whether altered hepatic mitochondrial energetics could explain the differential effects of high-fat diets with low or high ω6 polyunsaturated fatty acid content (lard vs. safflower oil) on the efficiency of body fat recovery (catch-up fat) during refeeding after caloric restriction. After 2 weeks of caloric restriction, rats were isocalorically refed with a low-fat diet (LF) or high-fat diets made from either lard or safflower oil for 1 week, and energy balance and body composition changes were assessed. Hepatic mitochondrial energetics were determined from measurements of liver mitochondrial mass, respiratory capacities, and proton leak. Compared to rats refed the LF, the groups refed high-fat diets showed lower energy expenditure and increased efficiency of fat gain; these differences were less marked with high-safflower oil than with high-lard diet. The increase in efficiency of catch-up fat by the high-fat diets could not be attributed to differences in liver mitochondrial activity. By contrast, the lower fat gain with high-safflower oil than with high-lard diet is accompanied by higher mitochondrial proton leak and increased proportion of arachidonic acid in mitochondrial membranes. In conclusion, the higher efficiency for catch-up fat on high-lard diet than on LF cannot be explained by altered hepatic mitochondrial energetics. By contrast, the ability of the high-safflower oil diet to produce a less pronounced increase in the efficiency of catch-up fat may partly reside in increased incorporation of arachidonic acid in hepatic mitochondrial membranes, leading to enhanced proton leak and mitochondrial uncoupling.

  9. A replication of "Education and catch-up in the Industrial Revolution" (American Economic Journal: Macroeconomics, 2011)

    OpenAIRE

    Edwards, Jeremy S. S.

    2017-01-01

    Although European economic history provides essentially no support for the view that education of the general population has a positive causal effect on economic growth, a recent paper by Becker, Hornung and Woessmann (Education and catch-up in the Industrial Revolution, 2011) claims that such education had a significant impact on Prussian industrialisation. The author shows that the instrumental variable they use to identify the causal effect of education is correlated with variables that in...

  10. A replication of "Education and catch-up in the industrial revolution" (American Economic Journal: Macroeconomics, 2011)

    OpenAIRE

    Edwards, Jeremy

    2018-01-01

    Although European economic history provides essentially no support for the view that education of the general population has a positive causal effect on economic growth, a recent paper by Becker, Hornung and Woessmann (Education and Catch-Up in the Industrial Revolution, 2011) claims that such education had a significant impact on Prussian industrialisation. The author shows that the instrumental variable BHW use to identify the causal effect of education is correlated with variables that inf...

  11. Shrimp by-catch reduction using a short funnel net, in the Gulf of Tehuantepec, South Pacific, Mexico

    Directory of Open Access Journals (Sweden)

    Saúl Sarmiento-Náfate

    2007-12-01

    Full Text Available Reduction of the by-catch in shrimp fishery is an important goal worldwide. In order to compare by-catch using the conventional trawling net and a newly designed short funnel net, nine cruises were carried out by the commercial fleet operating in the Gulf of Tehuantepec, Mexico. A total of 480 trawls were done (mean duration: 85 min. The experimental trawls using the modified net produced a 21.13-28.86 % by-catch reduction (confidence interval 95 %, a 3 % increase of shrimp catch, and a significant reduction on net recovery time. Rev. Biol. Trop. 55 (3-4: 889-897. Epub 2007 December, 28.La reducción de la fauna de acompañamiento del camarón (FAC es uno de los temas de discusión más importantes en la pesca mundial. Con la finalidad de contribuir en este tópico, se diseñó y utilizó una red de arrastre variando la longitud del túnel, y se comparó su efecto con el de una red tradicional; durante 9 cruceros de la flota comercial en el Golfo de Tehuantepec, México. Se realizó un total de 480 lances con promedio de 85 min cada uno. Los arrastres de la red modificada mostraron mejores resultados, con variación entre 21.13 a 28.86 %, con un 95 % de confianza de reducción de FAC, un incremento de 3 % en la captura de camarón, y una disminución del tiempo de recuperación, lo que sugiere un incremento en la eficiencia gracias al nuevo diseño.

  12. Engineering Task Plan for the Integrity Assessment Examination of Double-Contained Receiver Tanks (DCRT), Catch Tanks and Ancillary facilities

    International Nuclear Information System (INIS)

    BECKER, D.L.

    2000-01-01

    This Engineering Task Plan (ETP) presents the integrity assessment examination of three DCRTs, seven catch tanks, and two ancillary facilities located in the 200 East and West Areas of the Hanford Site. The integrity assessment examinations, as described in this ETP, will provide the necessary information to enable the independently qualified registered professional engineer (IQRPE) to assess the condition and integrity of these facilities. The plan is consistent with the Double-Shell Tank Waste Transfer Facilities Integrity Assessment Plan

  13. Identification of factors influencing shark catch and mortality in the Marshall Islands tuna longline fishery and management implications.

    Science.gov (United States)

    Bromhead, D; Clarke, S; Hoyle, S; Muller, B; Sharples, P; Harley, S

    2012-04-01

    Recent average annual catches of sharks by tuna longline vessels fishing in the Republic of the Marshall Islands (RMI) are estimated to be between 1583 and 2274 t. Although 22 shark species have been recorded by the observer programme for this fishery, 80% of the annual catch comprises only five species: blue shark Prionace glauca, silky shark Carcharhinus falciformis, bigeye thresher shark Alopias superciliosus, pelagic thresher shark Alopias pelagicus and oceanic whitetip shark Carcharhinus longimanus. Wire leaders (i.e. branch lines or traces) were also used by nearly all observed vessels. Generalized additive model (GAM)-based analyses of catch rates indicated that P. glauca and A. superciliosus are caught in higher numbers when vessels fish in relatively cooler waters, at night, close to the full moon, when the 27° C thermocline is close to the surface and during El Niño conditions. In contrast, C. falciformis, A. pelagicus and C. longimanus are caught in higher numbers when shark lines are used (all three species) or hooks are set at a shallow depth (A. pelagicus and C. longimanus and, also, P. glauca). These findings are generally consistent with current knowledge of these species' habitat preferences, movement and distribution. The results of these analyses were combined with information pertaining to shark condition and fate upon capture to compare the likely effectiveness of a range of potential measures for reducing shark mortality in the longline fishery. Of the options considered, the most effective would be to combine measures that reduce the catch rate (e.g. restrictions on the use of wire leaders, shark baits and shark lines) with measures that increase survival rates after post-capture release (e.g. finning bans). © 2012 Secrtariat of the Pacific Community. Journal of Fish Biology © 2012 The Fisheries Society of the British Isles.

  14. Long-term trajectory of some elasmobranch species off the Tuscany coasts (NW Mediterranean from 50 years of catch data

    Directory of Open Access Journals (Sweden)

    Alessandro Ligas

    2013-03-01

    Full Text Available The time series of elasmobranch catch rates off the Tuscany coasts (NW Mediterranean were investigated by means of min/max auto-correlation factor analysis in order to estimate variations in population abundance and evaluate the influence of environmental and anthropogenic factors. The analyses highlighted a general decreasing trend in the catch rates of sharks and skates from 1961 to the mid-1990s, mainly influenced by the increase in fishing effort. Since the 1990s, the EU Common Fishery Policy for the Mediterranean has promoted the reduction of fishing fleets through incentives to vessel demolition. The Porto S. Stefano trawl fleet has decreased by about 50%, leading to a decrease in fishing effort which seemed to be the most relevant factor affecting the increasing trend shown by the catch rates of Galeus melastomus, Scyliorhinus canicula and skates from 1991 to 2009. The elasmobranch assemblage did not undergo major shifts but the weighted frequency of occurrence shows that elasmobranchs were more frequent in the past. Particular caution should be paid in interpreting the recent rebound of some species as an early sign of recovery: trawl survey data and landing data show that over the last 50 years elasmobranch fauna have undergone a drastic decline and that recent rebounds are still far from a recovery to historical levels.

  15. Positive Catch & Economic Benefits of Periodic Octopus Fishery Closures: Do Effective, Narrowly Targeted Actions 'Catalyze' Broader Management?

    Science.gov (United States)

    Oliver, Thomas A; Oleson, Kirsten L L; Ratsimbazafy, Hajanaina; Raberinary, Daniel; Benbow, Sophie; Harris, Alasdair

    2015-01-01

    Eight years of octopus fishery records from southwest Madagascar reveal significant positive impacts from 36 periodic closures on: (a) fishery catches and (b) village fishery income, such that (c) economic benefits from increased landings outweigh costs of foregone catch. Closures covered ~20% of a village's fished area and lasted 2-7 months. Octopus landings and catch per unit effort (CPUE) significantly increased in the 30 days following a closure's reopening, relative to the 30 days before a closure (landings: +718%, poctopus fishery income doubled in the 30 days after a closure, relative to 30 days before (+132%, p<0.001, n = 28). Control villages not implementing a closure showed no increase in income after "no ban" closures and modest increases after "ban" closures. Villages did not show a significant decline in income during closure events. Landings in closure sites generated more revenue than simulated landings assuming continued open-access fishing at that site (27/36 show positive net earnings; mean +$305/closure; mean +57.7% monthly). Benefits accrued faster than local fishers' time preferences during 17-27 of the 36 closures. High reported rates of illegal fishing during closures correlated with poor economic performance. We discuss the implications of our findings for broader co-management arrangements, particularly for catalyzing more comprehensive management.

  16. Catch per unit efforts and impacts of gears on fish abundance in an oxbow lake ecosystem in Eastern India

    Directory of Open Access Journals (Sweden)

    Dipankar Ghosh

    2017-05-01

    Full Text Available Background: Oxbow lakes are abundant in indigenous fishes, but they are subject to unsustainable fishing practices, potential overexploitation, and indiscriminate use of fine-meshed fishing gear. To quantify the catch per unit effort (CPUE and impact of fishing gears on fish abundance, a survey was carried out in an oxbow lake in eastern India. Methods: The gear-wise CPUE for fish caught in per unit hour of operation was calculated by dividing the total sampling gear catch in biomass, which is the observed value of fish caught by a particular gear, by the total sampling effort hours. A value of P 71%. Cone-framed cast net hauled the maximum catch in biomass (31.51%, and gill nets contributed the maximum number of fish (64.92%. The lower CPUE values of line and hook, gill net, cone-framed cast net and long lines identified them as the most harmful among all gears. Conclusion: Indiscriminate use of gear, particularly line and hook, gill nets, cone-framed cast nets, and long lines, demands regulations and preventions concerning such gear to obtain higher fish abundance.

  17. No Weight Catch-Up Growth of SGA Infants Is Associated with Impaired Insulin Sensitivity during the Early Postnatal Period

    Directory of Open Access Journals (Sweden)

    Tong-yan Han

    2010-01-01

    Full Text Available Objective. To investigate the relationship between weight catch-up growth and insulin sensitivity in small for gestational age (SGA infants. Methods. Forty-four singleton SGA subjects met the inclusion criteria and finished-3-month followup. Body weight, length, fasting glucose, and fasting insulin (FI levels were measured at 3 days and 3 months. Insulin sensitivity was evaluated by FI and homeostasis model assessment (HOMA. Results. According to the change of weight Z-score, forty-four subjects were divided into two groups: noncatch-up growth (NCUG and catch-up growth (CUG. By 3 months of age, the body weight, body length and BMI of NCUG group were significantly lower than those of CUG group. The FI and HOMA were significantly higher in NCUG group. The change of weight Z-score during 3 months was inversely related to the HOMA at 3 months. Conclusion. Our data exemplified that no weight catch-up growth during the first 3 months was associated with impaired insulin sensitivity in SGA infants.

  18. The validity of testicular catch-up growth and serum FSH levels in the long-term postoperative assessment of laparoscopic varicocele correction in adolescents

    Directory of Open Access Journals (Sweden)

    Aniruddh Deshpande

    2011-01-01

    Conclusions: Testicular catch-up growth may not be a reliable postoperative assessment criterion by itself. Serum FSH levels may be of value in detecting suboptimal outcomes of varicocele surgery in adolescents.

  19. Behavioral inferences from the statistical distribution of commercial catch: patterns of targeting in the landings of the Dutch beam trawler fleet

    NARCIS (Netherlands)

    Gillis, D.M.; Rijnsdorp, A.D.; Poos, J.J.

    2008-01-01

    The objective identification of targeting behavior in multispecies fisheries is critical to the development and evaluation of management measures. Here, we illustrate how the statistical distribution of commercial catches can provide information on species preference that is consistent with economic

  20. Recreational fishery catch and effort estimates for large pelagic species from Maine through Virginia from 2002-06-01 to 2014-10-31 (NCEI Accession 0137959)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — On the Atlantic Coast from Maine to Virginia, NOAA Fisheries uses the Large Pelagics Survey (LPS) to measure the total recreational catch of large pelagics fish...