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Sample records for progerin-interactive partner proteins

  1. Inferring interaction partners from protein sequences

    CERN Document Server

    Bitbol, Anne-Florence; Colwell, Lucy J; Wingreen, Ned S

    2016-01-01

    Specific protein-protein interactions are crucial in the cell, both to ensure the formation and stability of multi-protein complexes, and to enable signal transduction in various pathways. Functional interactions between proteins result in coevolution between the interaction partners. Hence, the sequences of interacting partners are correlated. Here we exploit these correlations to accurately identify which proteins are specific interaction partners from sequence data alone. Our general approach, which employs a pairwise maximum entropy model to infer direct couplings between residues, has been successfully used to predict the three-dimensional structures of proteins from sequences. Building on this approach, we introduce an iterative algorithm to predict specific interaction partners from among the members of two protein families. We assess the algorithm's performance on histidine kinases and response regulators from bacterial two-component signaling systems. The algorithm proves successful without any a pri...

  2. SPPS: a sequence-based method for predicting probability of protein-protein interaction partners.

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    Xinyi Liu

    Full Text Available The molecular network sustained by different types of interactions among proteins is widely manifested as the fundamental driving force of cellular operations. Many biological functions are determined by the crosstalk between proteins rather than by the characteristics of their individual components. Thus, the searches for protein partners in global networks are imperative when attempting to address the principles of biology.We have developed a web-based tool "Sequence-based Protein Partners Search" (SPPS to explore interacting partners of proteins, by searching over a large repertoire of proteins across many species. SPPS provides a database containing more than 60,000 protein sequences with annotations and a protein-partner search engine in two modes (Single Query and Multiple Query. Two interacting proteins of human FBXO6 protein have been found using the service in the study. In addition, users can refine potential protein partner hits by using annotations and possible interactive network in the SPPS web server.SPPS provides a new type of tool to facilitate the identification of direct or indirect protein partners which may guide scientists on the investigation of new signaling pathways. The SPPS server is available to the public at http://mdl.shsmu.edu.cn/SPPS/.

  3. Flexibility of PCNA-protein interface accommodates differential binding partners.

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    Pedley, Anthony M; Lill, Markus A; Davisson, V Jo

    2014-01-01

    The expanding roles of PCNA in functional assembly of DNA replication and repair complexes motivated investigation of the structural and dynamic properties guiding specificity of PCNA-protein interactions. A series of biochemical and computational analyses were combined to evaluate the PIP Box recognition features impacting complex formation. The results indicate subtle differences in topological and molecular descriptors distinguishing both affinity and stoichiometry of binding among PCNA-peptide complexes through cooperative effects. These features were validated using peptide mimics of p85α and Akt, two previously unreported PCNA binding partners. This study characterizes for the first time a reverse PIP Box interaction with PCNA. Small molecule ligand binding at the PIP Box interaction site confirmed the adaptive nature of the protein in dictating overall shape and implicates allosterism in transmitting biological effects.

  4. Flexibility of PCNA-protein interface accommodates differential binding partners.

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    Anthony M Pedley

    Full Text Available The expanding roles of PCNA in functional assembly of DNA replication and repair complexes motivated investigation of the structural and dynamic properties guiding specificity of PCNA-protein interactions. A series of biochemical and computational analyses were combined to evaluate the PIP Box recognition features impacting complex formation. The results indicate subtle differences in topological and molecular descriptors distinguishing both affinity and stoichiometry of binding among PCNA-peptide complexes through cooperative effects. These features were validated using peptide mimics of p85α and Akt, two previously unreported PCNA binding partners. This study characterizes for the first time a reverse PIP Box interaction with PCNA. Small molecule ligand binding at the PIP Box interaction site confirmed the adaptive nature of the protein in dictating overall shape and implicates allosterism in transmitting biological effects.

  5. New partner proteins containing novel internal recognition motif for human glutaminase interacting protein (hGIP).

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    Zencir, Sevil; Banerjee, Monimoy; Dobson, Melanie J; Ayaydin, Ferhan; Fodor, Elfrieda Ayaydin; Topcu, Zeki; Mohanty, Smita

    2013-03-01

    Regulation of gene expression in cells is mediated by protein-protein, DNA-protein and receptor-ligand interactions. PDZ (PSD-95/Discs-large/ZO-1) domains are protein-protein interaction modules. PDZ-containing proteins function in the organization of multi-protein complexes controlling spatial and temporal fidelity of intracellular signaling pathways. In general, PDZ proteins possess multiple domains facilitating distinct interactions. The human glutaminase interacting protein (hGIP) is an unusual PDZ protein comprising entirely of a single PDZ domain and plays pivotal roles in many cellular processes through its interaction with the C-terminus of partner proteins. Here, we report the identification by yeast two-hybrid screening of two new hGIP-interacting partners, DTX1 and STAU1. Both proteins lack the typical C-terminal PDZ recognition motif but contain a novel internal hGIP recognition motif recently identified in a phage display library screen. Fluorescence resonance energy transfer and confocal microscopy analysis confirmed the in vivo association of hGIP with DTX1 and STAU1 in mammalian cells validating the previous discovery of S/T-X-V/L-D as a consensus internal motif for hGIP recognition. Similar to hGIP, DTX1 and STAU1 have been implicated in neuronal function. Identification of these new interacting partners furthers our understanding of GIP-regulated signaling cascades and these interactions may represent potential new drug targets in humans. Copyright © 2013 Elsevier Inc. All rights reserved.

  6. Biological significance and topological basis of aquaporin-partnering protein-protein interactions.

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    Ji, Hongtao; Dong, Hansong

    2015-01-01

    Aquaporins (AQPs) are intramolecular channels essential for transport of H2O, CO2, and other small substrates across membranes. Through this function, AQPs can modulate CO2 uptake and assimilation in plants and regulate water relations and many other physiological processes in all living organisms. To execute their physiological roles, AQPs may experience 3 types of hetero-molecular interaction, between AQPs and their kinases; between AQP isoforms; and between AQPs and other proteins that are neither AQPs nor kinases. Interacting with non-AQP non-kinase proteins may enable AQPs to extend their functions beyond substrate transport, and most fascinatingly, to serve as a gateway control for translocation of virulence effectors from pathogenic bacteria into the cytosol of eukaryotic cells. In this mini review, we will summarize the latter 2 types of interaction and discuss the physiological and/or pathological significance. We will also discuss a research angle to elucidate the structural basis of AQP-partnering protein interactions.

  7. Split green fluorescent protein as a modular binding partner for protein crystallization.

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    Nguyen, Hau B; Hung, Li-Wei; Yeates, Todd O; Terwilliger, Thomas C; Waldo, Geoffrey S

    2013-12-01

    A modular strategy for protein crystallization using split green fluorescent protein (GFP) as a crystallization partner is demonstrated. Insertion of a hairpin containing GFP β-strands 10 and 11 into a surface loop of a target protein provides two chain crossings between the target and the reconstituted GFP compared with the single connection afforded by terminal GFP fusions. This strategy was tested by inserting this hairpin into a loop of another fluorescent protein, sfCherry. The crystal structure of the sfCherry-GFP(10-11) hairpin in complex with GFP(1-9) was determined at a resolution of 2.6 Å. Analysis of the complex shows that the reconstituted GFP is attached to the target protein (sfCherry) in a structurally ordered way. This work opens the way to rapidly creating crystallization variants by reconstituting a target protein bearing the GFP(10-11) hairpin with a variety of GFP(1-9) mutants engineered for favorable crystallization.

  8. Identification of actin binding protein, ABP-280, as a binding partner of human Lnk adaptor protein.

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    He, X; Li, Y; Schembri-King, J; Jakes, S; Hayashi, J

    2000-08-01

    Human Lnk (hLnk) is an adaptor protein with multiple functional domains that regulates T cell activation signaling. In order to identify cellular Lnk binding partners, a yeast two-hybrid screening of human spleen cDNA library was carried out using human hLnk as bait. A polypeptide sequence identical to the C-terminal segment of the actin binding protein (ABP-280) was identified as a hLnk binding protein. The expressed hLnk and the FLAG tagged C-terminal 673 amino acid residues of ABP-280 or the endogenous ABP-280 in COS-7 cells could be co-immunoprecipitated using antibodies either to hLnk, FLAG or ABP-280, respectively. Furthermore, immunofluorescence confocal microscope showed that hLnk and ABP-280 co-localized at the plasma membrane and at juxtanuclear region of COS-7 cells. In Jurkat cells, the endogenous hLnk also associates with the endogenous ABP-280 indicating that the association of these two proteins is physiological. The interacting domains of both proteins were mapped using yeast two-hybrid assays. Our results indicate that hLnk binds to the residues 2006-2454 (repeats 19-23C) of ABP-280. The domain in hLnk that associates with ABP-280 was mapped to an interdomain region of 56 amino acids between pleckstrin homology and Src homology 2 domains. These results suggest that hLnk may exert its regulatory role through its association with ABP-280.

  9. Protein social behavior makes a stronger signal for partner identification than surface geometry

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    Laine, Elodie

    2016-01-01

    ABSTRACT Cells are interactive living systems where proteins movements, interactions and regulation are substantially free from centralized management. How protein physico‐chemical and geometrical properties determine who interact with whom remains far from fully understood. We show that characterizing how a protein behaves with many potential interactors in a complete cross‐docking study leads to a sharp identification of its cellular/true/native partner(s). We define a sociability index, or S‐index, reflecting whether a protein likes or not to pair with other proteins. Formally, we propose a suitable normalization function that accounts for protein sociability and we combine it with a simple interface‐based (ranking) score to discriminate partners from non‐interactors. We show that sociability is an important factor and that the normalization permits to reach a much higher discriminative power than shape complementarity docking scores. The social effect is also observed with more sophisticated docking algorithms. Docking conformations are evaluated using experimental binding sites. These latter approximate in the best possible way binding sites predictions, which have reached high accuracy in recent years. This makes our analysis helpful for a global understanding of partner identification and for suggesting discriminating strategies. These results contradict previous findings claiming the partner identification problem being solvable solely with geometrical docking. Proteins 2016; 85:137–154. © 2016 Wiley Periodicals, Inc. PMID:27802579

  10. A novel fusion partner for enhanced secretion of recombinant proteins in Saccharomyces cerevisiae.

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    Bae, Jung-Hoon; Sung, Bong Hyun; Seo, Jeong-Woo; Kim, Chul Ho; Sohn, Jung-Hoon

    2016-12-01

    Expressing proteins with fusion partners improves yield and simplifies the purification process. We developed a novel fusion partner to improve the secretion of heterologous proteins that are otherwise poorly excreted in yeast. The VOA1 (YGR106C) gene of Saccharomyces cerevisiae encodes a subunit of vacuolar ATPase. We found that C-terminally truncated Voa1p was highly secreted into the culture medium, even when fused with rarely secreted heterologous proteins such as human interleukin-2 (hIL-2). Deletion mapping of C-terminally truncated Voa1p, identified a hydrophilic 28-amino acid peptide (HL peptide) that was responsible for the enhanced secretion of target protein. A purification tag and a protease cleavage site were added to use HL peptide as a multi-purpose fusion partner. The utility of this system was tested via the expression and purification of various heterologous proteins. In many cases, the yield of target proteins fused with the peptide was significantly increased, and fusion proteins could be directly purified with affinity chromatography. The fusion partner was removed by in vitro processing, and intact proteins were purified by re-application of samples to affinity chromatography.

  11. Strumpellin and Spartin, Hereditary Spastic Paraplegia Proteins, are Binding Partners

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    Jiali Zhao

    2015-01-01

    Full Text Available Hereditary spastic paraplegia (HSP is one of the most heterogeneous neurodegenerative diseases with more than 50 identified genes causing a relatively stereotypical phenotypic presentation. Recent studies of HSP pathogenesis have suggested the existence of shared biochemical pathways that are crucial for axonal maintenance and degeneration. We explored possible interactions of several proteins associated with this condition. Here we report interactions of endogenous and overexpressed strumpellin with another HSP-associated protein, spartin. This biochemical interaction does not appear to be a part of the Wiskott-Aldrich syndrome protein and Scar homologue (WASH complex because spartin is not co-immunoprecipitated with WASH1 protein. The spartin–-strumpellin association does not require the presence of the microtubule interacting and trafficking domain of spartin. Over-expression of mutant forms of strumpellin with the introduced HSP-causing mutations does not alter the colocalization of these two proteins. Knockdown of strumpellin in cultured cortical rat neurons interferes with development of neuronal branching and results in reduced expression of endogenous spartin. Proteosomal inhibition stabilized the levels of spartin and WASH 1 proteins, supporting increased spartin degradation in the absence of strumpellin.

  12. Profiling of serum proteins influenced by warm partner contact in healthy couples.

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    Matsunaga, Masahiro; Sato, Sayaka; Isowa, Tokiko; Tsuboi, Hirohito; Konagaya, Toshihiro; Kaneko, Hiroshi; Ohira, Hideki

    2009-01-01

    Warm physical contact may positively influence our health and well-being; however, it has not been investigated yet whether serum proteins are influenced by warm physical contact in healthy couples. In this study, we focused on psychological and physiological effects of warm partner contact in healthy couples. When participants freely kissed and hugged their romantic partners, they were asked to subjectively evaluate their present emotions. Furthermore, changes of serum proteins were determined by using ProteinChip surface enhanced laser desorption/ionization-time-of-flight-mass spectrometry (SELDI-TOF-MS). We characterized these proteins by using biochemical techniques combined with gel filtration high performance liquid chromatography (HPLC), reverse-phase HPLC, and sequencing analyses. Romantic couples became happier and less irritated after kissing and hugging. Accompanying these psychological changes, SELDI-TOF-MS indicated that the intensities of 66-k Da, 11.7-k Da, and 5.9-k Da serum proteins were increased. These proteins were identified as serum albumin and beta2-microglobulin, and probably fibrinogen fragment. The feeling of happiness positively correlated and the feeling of irritation negatively correlated with intensities of serum albumin and beta2-microglobulin. These results suggest that psychological stress may be reduced and we may feel happiness when we kiss and hug a romantic partner. Furthermore, these results also suggest that warm partner contact influences peripheral circulating proteins, more importantly, may promote health and well-being.

  13. Fanconi Anemia Proteins and Their Interacting Partners: A Molecular Puzzle

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    Kaddar, Tagrid; Carreau, Madeleine

    2012-01-01

    In recent years, Fanconi anemia (FA) has been the subject of intense investigations, primarily in the DNA repair research field. Many discoveries have led to the notion of a canonical pathway, termed the FA pathway, where all FA proteins function sequentially in different protein complexes to repair DNA cross-link damages. Although a detailed architecture of this DNA cross-link repair pathway is emerging, the question of how a defective DNA cross-link repair process translates into the disease phenotype is unresolved. Other areas of research including oxidative metabolism, cell cycle progression, apoptosis, and transcriptional regulation have been studied in the context of FA, and some of these areas were investigated before the fervent enthusiasm in the DNA repair field. These other molecular mechanisms may also play an important role in the pathogenesis of this disease. In addition, several FA-interacting proteins have been identified with roles in these “other” nonrepair molecular functions. Thus, the goal of this paper is to revisit old ideas and to discuss protein-protein interactions related to other FA-related molecular functions to try to give the reader a wider perspective of the FA molecular puzzle. PMID:22737580

  14. Effects of partner proteins on BCA2 RING ligase activity

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    Bacopulos Stephanie

    2012-02-01

    Full Text Available Abstract Background BCA2 is an E3 ligase linked with hormone responsive breast cancers. We have demonstrated previously that the RING E3 ligase BCA2 has autoubiquitination activity and is a very unstable protein. Previously, only Rab7, tetherin, ubiquitin and UBC9 were known to directly interact with BCA2. Methods Here, additional BCA2 binding proteins were found using yeast two-hybrid and bacterial-II-hybrid screening techniques with Human breast and HeLa cDNA libraries. Co-expression of these proteins was analyzed through IHC of TMAs. Investigation of the molecular interactions and effects were examined through a series of in vivo and in vitro assays. Results Ten unique BCA2 interacting proteins were identified, two of which were hHR23a and 14-3-3sigma. Both hHR23a and 14-3-3sigma are co-expressed with BCA2 in breast cancer cell lines and patient breast tumors (n = 105. hHR23a and BCA2 expression was significantly correlated (P = Conclusions The interaction between BCA2 and hHR23a in breast cancer cells stabilizes BCA2. High expression of BCA2 is correlated with grade in breast cancer, suggesting regulation of this E3 ligase is important to cancer progression.

  15. Brain transcriptome-wide screen for HIV-1 Nef protein interaction partners reveals various membrane-associated proteins.

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    Ellen C Kammula

    Full Text Available HIV-1 Nef protein contributes essentially to the pathology of AIDS by a variety of protein-protein-interactions within the host cell. The versatile functionality of Nef is partially attributed to different conformational states and posttranslational modifications, such as myristoylation. Up to now, many interaction partners of Nef have been identified using classical yeast two-hybrid screens. Such screens rely on transcriptional activation of reporter genes in the nucleus to detect interactions. Thus, the identification of Nef interaction partners that are integral membrane proteins, membrane-associated proteins or other proteins that do not translocate into the nucleus is hampered. In the present study, a split-ubiquitin based yeast two-hybrid screen was used to identify novel membrane-localized interaction partners of Nef. More than 80% of the hereby identified interaction partners of Nef are transmembrane proteins. The identified hits are GPM6B, GPM6A, BAP31, TSPAN7, CYB5B, CD320/TCblR, VSIG4, PMEPA1, OCIAD1, ITGB1, CHN1, PH4, CLDN10, HSPA9, APR-3, PEBP1 and B3GNT, which are involved in diverse cellular processes like signaling, apoptosis, neurogenesis, cell adhesion and protein trafficking or quality control. For a subfraction of the hereby identified proteins we present data supporting their direct interaction with HIV-1 Nef. We discuss the results with respect to many phenotypes observed in HIV infected cells and patients. The identified Nef interaction partners may help to further elucidate the molecular basis of HIV-related diseases.

  16. Identification of new interacting partners of the shuttling protein ubinuclein (Ubn-1)

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    Lupo, Julien [Unit of Virus Host Cell Interactions (UVHCI), UMI 3265 UJF-EMBL-CNRS, 6 rue Jules Horowitz, BP 181, F-38042 Grenoble Cedex 9 (France); CHU de Grenoble, BP217, 38043 Grenoble Cedex 9 (France); Conti, Audrey [Unit of Virus Host Cell Interactions (UVHCI), UMI 3265 UJF-EMBL-CNRS, 6 rue Jules Horowitz, BP 181, F-38042 Grenoble Cedex 9 (France); Sueur, Charlotte [Unit of Virus Host Cell Interactions (UVHCI), UMI 3265 UJF-EMBL-CNRS, 6 rue Jules Horowitz, BP 181, F-38042 Grenoble Cedex 9 (France); CHU de Grenoble, BP217, 38043 Grenoble Cedex 9 (France); Coly, Pierre-Alain [Unit of Virus Host Cell Interactions (UVHCI), UMI 3265 UJF-EMBL-CNRS, 6 rue Jules Horowitz, BP 181, F-38042 Grenoble Cedex 9 (France); Coute, Yohann [CEA, IRTSV, Laboratoire Biologie a Grande Echelle, F-38054 Grenoble (France); INSERM, U1038, F-38054 Grenoble (France); Universite Joseph Fourier, Grenoble 1, F-38000 Grenoble Cedex 09 (France); Hunziker, Walter [Institute of Molecular and Cell Biology, Epithelial Cell Biology Laboratory, Singapore 1386473 (Singapore); Burmeister, Wim P. [Unit of Virus Host Cell Interactions (UVHCI), UMI 3265 UJF-EMBL-CNRS, 6 rue Jules Horowitz, BP 181, F-38042 Grenoble Cedex 9 (France); Germi, Raphaelle [Unit of Virus Host Cell Interactions (UVHCI), UMI 3265 UJF-EMBL-CNRS, 6 rue Jules Horowitz, BP 181, F-38042 Grenoble Cedex 9 (France); CHU de Grenoble, BP217, 38043 Grenoble Cedex 9 (France); Manet, Evelyne; Gruffat, Henri [INSERM U758, Unite de Virologie humaine, Lyon, 46 allee d' Italie F-69007 France (France); Ecole Normale Superieure de Lyon, F-69007 France (France); Universite Lyon1, F-69007, Lyon (France); and others

    2012-03-10

    We have previously characterized ubinuclein (Ubn-1) as a NACos (Nuclear and Adherent junction Complex components) protein which interacts with viral or cellular transcription factors and the tight junction (TJ) protein ZO-1. The purpose of the present study was to get more insights on the binding partners of Ubn-1, notably those present in the epithelial junctions. Using an in vivo assay of fluorescent protein-complementation assay (PCA), we demonstrated that the N-terminal domains of the Ubn-1 and ZO-1 proteins triggered a functional interaction inside the cell. Indeed, expression of both complementary fragments of venus fused to the N-terminal parts of Ubn-1 and ZO-1 was able to reconstitute a fluorescent venus protein. Furthermore, nuclear expression of the chimeric Ubn-1 triggered nuclear localization of the chimeric ZO-1. We could localize this interaction to the PDZ2 domain of ZO-1 using an in vitro pull-down assay. More precisely, a 184-amino acid region (from amino acids 39 to 223) at the N-terminal region of Ubn-1 was responsible for the interaction with the PDZ2 domain of ZO-1. Co-imunoprecipitation and confocal microscopy experiments also revealed the tight junction protein cingulin as a new interacting partner of Ubn-1. A proteomic approach based on mass spectrometry analysis (MS) was then undertaken to identify further binding partners of GST-Ubn-1 fusion protein in different subcellular fractions of human epithelial HT29 cells. LYRIC (Lysine-rich CEACAM1-associated protein) and RACK-1 (receptor for activated C-kinase) proteins were validated as bona fide interacting partners of Ubn-1. Altogether, these results suggest that Ubn-1 is a scaffold protein influencing protein subcellular localization and is involved in several processes such as cell-cell contact signalling or modulation of gene activity.

  17. Positional effects of fusion partners on the yield and solubility of MBP fusion proteins.

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    Raran-Kurussi, Sreejith; Keefe, Karina; Waugh, David S

    2015-06-01

    Escherichia coli maltose-binding protein (MBP) is exceptionally effective at promoting the solubility of its fusion partners. However, there are conflicting reports in the literature claiming that (1) MBP is an effective solubility enhancer only when it is joined to the N-terminus of an aggregation-prone passenger protein, and (2) MBP is equally effective when fused to either end of the passenger. Here, we endeavor to resolve this controversy by comparing the solubility of a diverse set of MBP fusion proteins that, unlike those analyzed in previous studies, are identical in every way except for the order of the two domains. The results indicate that fusion proteins with an N-terminal MBP provide an excellent solubility advantage along with more robust expression when compared to analogous fusions in which MBP is the C-terminal fusion partner. We find that only intrinsically soluble passenger proteins (i.e., those not requiring a solubility enhancer) are produced as soluble fusions when they precede MBP. We also report that even subtle differences in inter-domain linker sequences can influence the solubility of fusion proteins. Published by Elsevier Inc.

  18. Bound water at protein-protein interfaces: partners, roles and hydrophobic bubbles as a conserved motif.

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    Mostafa H Ahmed

    Full Text Available There is a great interest in understanding and exploiting protein-protein associations as new routes for treating human disease. However, these associations are difficult to structurally characterize or model although the number of X-ray structures for protein-protein complexes is expanding. One feature of these complexes that has received little attention is the role of water molecules in the interfacial region.A data set of 4741 water molecules abstracted from 179 high-resolution (≤ 2.30 Å X-ray crystal structures of protein-protein complexes was analyzed with a suite of modeling tools based on the HINT forcefield and hydrogen-bonding geometry. A metric termed Relevance was used to classify the general roles of the water molecules.The water molecules were found to be involved in: a (bridging interactions with both proteins (21%, b favorable interactions with only one protein (53%, and c no interactions with either protein (26%. This trend is shown to be independent of the crystallographic resolution. Interactions with residue backbones are consistent for all classes and account for 21.5% of all interactions. Interactions with polar residues are significantly more common for the first group and interactions with non-polar residues dominate the last group. Waters interacting with both proteins stabilize on average the proteins' interaction (-0.46 kcal mol(-1, but the overall average contribution of a single water to the protein-protein interaction energy is unfavorable (+0.03 kcal mol(-1. Analysis of the waters without favorable interactions with either protein suggests that this is a conserved phenomenon: 42% of these waters have SASA ≤ 10 Å(2 and are thus largely buried, and 69% of these are within predominantly hydrophobic environments or "hydrophobic bubbles". Such water molecules may have an important biological purpose in mediating protein-protein interactions.

  19. Identification of potential protein partners that bind to the variant surface glycoprotein in Trypanosoma equiperdum.

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    Carrasquel, Liomary M; Escalona, José L; Acosta-Serrano, Alvaro; Guo, Yurong; Bubis, José

    2017-06-01

    Trypanosoma equiperdum possesses a dense coat of a variant surface glycoprotein (VSG) that is used to evade the host immune response by a process known as antigenic variation. Soluble and membrane forms of the predominant VSG from the Venezuelan T. equiperdum TeAp-N/D1 strain (sVSG and mVSG, respectively) were purified to homogeneity; and antibodies against sVSG and mVSG were raised, isolated, and employed to produce anti-idiotypic antibodies that structurally mimic the VSG surface. Prospective VSG-binding partners were initially detected by far-Western blots, and then by immunoblots using the generated anti-idiotypic antibodies. Polypeptides of ~80 and 55 kDa were isolated when anti-idiotypic antibodies-Sepharose affinity matrixes were used as baits. Mass spectrometry sequencing yielded hits with various proteins from Trypanosoma brucei such as heat-shock protein 70, tryparedoxin peroxidase, VSG variants, expression site associated gene product 6, and two hypothetical proteins. In addition, a possible interaction with a protein homologous to the glutamic acid/alanine-rich protein from Trypanosoma congolense was also found. These results indicate that the corresponding orthologous gene products are candidates for VSG-interacting proteins in T. equiperdum.

  20. Gene regulation by translational inhibition is determined by Dicer partnering proteins.

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    Reis, Rodrigo S; Hart-Smith, Gene; Eamens, Andrew L; Wilkins, Marc R; Waterhouse, Peter M

    2015-02-09

    MicroRNAs (miRNAs) are small regulatory RNAs produced by Dicer proteins that regulate gene expression in development and adaptive responses to the environment(1-4). In animals, the degree of base pairing between a miRNA and its target messenger RNA seems to determine whether the regulation occurs through cleavage or translation inhibition(1). In contrast, the selection of regulatory mechanisms is independent of the degree of mismatch between a plant miRNA and its target transcript(5). However, the components and mechanism(s) that determine whether a plant miRNA ultimately regulates its targets by guiding cleavage or translational inhibition are unknown(6). Here we show that the form of regulatory action directed by a plant miRNA is determined by DRB2, a DICER-LIKE1 (DCL1) partnering protein. The dependence of DCL1 on DRB1 for miRNA biogenesis is well characterized(7-9), but we show that it is only required for miRNA-guided transcript cleavage. We found that DRB2 determines miRNA-guided translational inhibition and represses DRB1 expression, thereby allowing the active selection of miRNA regulatory action. Furthermore, our results reveal that the core silencing proteins ARGONAUTE1 (AGO1) and SERRATE (SE) are highly regulated by miRNA-guided translational inhibition. DRB2 has been remarkably conserved throughout plant evolution, raising the possibility that translational repression is the ancient form of miRNA-directed gene regulation in plants, and that Dicer partnering proteins, such as human TRBP, might play a similar role in other eukaryotic systems.

  1. Begomoviral Movement Protein Effects in Human and Plant Cells: Towards New Potential Interaction Partners

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    Krapp, Susanna; Schuy, Christian; Greiner, Eva; Stephan, Irina; Alberter, Barbara; Funk, Christina; Marschall, Manfred; Wege, Christina; Bailer, Susanne M.; Kleinow, Tatjana; Krenz, Björn

    2017-01-01

    Geminiviral single-stranded circular DNA genomes replicate in nuclei so that the progeny DNA has to cross both the nuclear envelope and the plasmodesmata for systemic spread within plant tissues. For intra- and intercellular transport, two proteins are required: a nuclear shuttle protein (NSP) and a movement protein (MP). New characteristics of ectopically produced Abutilon mosaic virus (AbMV) MP (MPAbMV), either authentically expressed or fused to a yellow fluorescent protein or epitope tags, respectively, were determined by localization studies in mammalian cell lines in comparison to plant cells. Wild-type MPAbMV and the distinct MPAbMV: reporter protein fusions appeared as curled threads throughout mammalian cells. Co-staining with cytoskeleton markers for actin, intermediate filaments, or microtubules identified these threads as re-organized microtubules. These were, however, not stabilized by the viral MP, as demonstrated by nocodazole treatment. The MP of a related bipartite New World begomovirus, Cleome leaf crumple virus (ClLCrV), resulted in the same intensified microtubule bundling, whereas that of a nanovirus did not. The C-terminal section of MPAbMV, i.e., the protein’s oligomerization domain, was dispensable for the effect. However, MP expression in plant cells did not affect the microtubules network. Since plant epidermal cells are quiescent whilst mammalian cells are proliferating, the replication-associated protein RepAbMV protein was then co-expressed with MPAbMV to induce cell progression into S-phase, thereby inducing distinct microtubule bundling without MP recruitment to the newly formed threads. Co-immunoprecipitation of MPAbMV in the presence of RepAbMV, followed by mass spectrometry identified potential novel MPAbMV-host interaction partners: the peptidyl-prolyl cis-trans isomerase NIMA-interacting 4 (Pin4) and stomatal cytokinesis defective 2 (SCD2) proteins. Possible roles of these putative interaction partners in the begomoviral life

  2. Begomoviral Movement Protein Effects in Human and Plant Cells: Towards New Potential Interaction Partners

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    Susanna Krapp

    2017-11-01

    Full Text Available Geminiviral single-stranded circular DNA genomes replicate in nuclei so that the progeny DNA has to cross both the nuclear envelope and the plasmodesmata for systemic spread within plant tissues. For intra- and intercellular transport, two proteins are required: a nuclear shuttle protein (NSP and a movement protein (MP. New characteristics of ectopically produced Abutilon mosaic virus (AbMV MP (MPAbMV, either authentically expressed or fused to a yellow fluorescent protein or epitope tags, respectively, were determined by localization studies in mammalian cell lines in comparison to plant cells. Wild-type MPAbMV and the distinct MPAbMV: reporter protein fusions appeared as curled threads throughout mammalian cells. Co-staining with cytoskeleton markers for actin, intermediate filaments, or microtubules identified these threads as re-organized microtubules. These were, however, not stabilized by the viral MP, as demonstrated by nocodazole treatment. The MP of a related bipartite New World begomovirus, Cleome leaf crumple virus (ClLCrV, resulted in the same intensified microtubule bundling, whereas that of a nanovirus did not. The C-terminal section of MPAbMV, i.e., the protein’s oligomerization domain, was dispensable for the effect. However, MP expression in plant cells did not affect the microtubules network. Since plant epidermal cells are quiescent whilst mammalian cells are proliferating, the replication-associated protein RepAbMV protein was then co-expressed with MPAbMV to induce cell progression into S-phase, thereby inducing distinct microtubule bundling without MP recruitment to the newly formed threads. Co-immunoprecipitation of MPAbMV in the presence of RepAbMV, followed by mass spectrometry identified potential novel MPAbMV-host interaction partners: the peptidyl-prolyl cis-trans isomerase NIMA-interacting 4 (Pin4 and stomatal cytokinesis defective 2 (SCD2 proteins. Possible roles of these putative interaction partners in the

  3. Human Peroxiredoxins 1 and 2 and Their Interacting Protein Partners; Through Structure Toward Functions of Biological Complexes.

    Science.gov (United States)

    Bertoldi, Mariarita

    2016-01-01

    Since their discovery in the mid-nineties, peroxiredoxins have drawn much attention and the number of papers publications on different Prxs has been multiplied. The rise in interest in this topic is probably due, at least in part, to the large and further increasing functions attributed to the members of this family of ubiquitous proteins, including many redox and non-redox physiological functions. This review presents a Since their discovery in the mid-nineties, peroxiredoxins have drawn much attention and the number of publications on different Prxs has been multiplied. The rise in interest in this topic is probably due, at least in part, to the large and further increasing functions attributed to the members of this family of ubiquitous proteins, including many redox and non-redox physiological functions. This review presents a literature survey of the protein partners of the human Peroxiredoxin-1 and Peroxiredoxin- 2 of the Peroxiredoxin 1 subfamily, the most abundant class. Three sequence motifs, or combinations thereof, were found in the protein partners, namely, CXXC, PXXP, and LXXLL. These findings are discussed in light of i) protein partner localization, function and biological pathways and ii) the peroxiredoxins regions important for partner interaction, as revealed by the Peroxiredoxin-1-Sulfiredoxin-1 complex structure. The outcome of these analyses is expected to unravel some common molecular bases underlying peroxiredoxins propensity to bind a partner, as well as to propose a functional role for this interaction that could help to widen the biological role of this important class of enzymes.

  4. Interacting protein partners of Arabidopsis RNA binding protein AtRBP45b

    Science.gov (United States)

    RNA binding proteins (RBPs) are important players in post-transcriptional gene regulation and shown to play an important role in normal development and in response to environmental perturbations. Arabidopsis RBP, AtRBP45b with triple RNA recognition motifs (RRMs) have are closely related to the yeas...

  5. Metabolic labeling enables selective photocrosslinking of O-GlcNAc-modified proteins to their binding partners

    Science.gov (United States)

    Yu, Seok-Ho; Boyce, Michael; Wands, Amberlyn M.; Bond, Michelle R.; Bertozzi, Carolyn R.; Kohler, Jennifer J.

    2012-01-01

    O-linked β-N-acetylglucosamine (O-GlcNAc) is a reversible posttranslational modification found on hundreds of nuclear and cytoplasmic proteins in higher eukaryotes. Despite its ubiquity and essentiality in mammals, functional roles for the O-GlcNAc modification remain poorly defined. Here we develop a combined genetic and chemical approach that enables introduction of the diazirine photocrosslinker onto the O-GlcNAc modification in cells. We engineered mammalian cells to produce diazirine-modified O-GlcNAc by expressing a mutant form of UDP-GlcNAc pyrophosphorylase and subsequently culturing these cells with a cell-permeable, diazirine-modified form of GlcNAc-1-phosphate. Irradiation of cells with UV light activated the crosslinker, resulting in formation of covalent bonds between O-GlcNAc-modified proteins and neighboring molecules, which could be identified by mass spectrometry. We used this method to identify interaction partners for the O-GlcNAc-modified FG-repeat nucleoporins. We observed crosslinking between FG-repeat nucleoporins and nuclear transport factors, suggesting that O-GlcNAc residues are intimately associated with essential recognition events in nuclear transport. Further, we propose that the method reported here could find widespread use in investigating the functional consequences of O-GlcNAcylation. PMID:22411826

  6. Chimeric DCL1-Partnering Proteins Provide Insights into the MicroRNA Pathway.

    Science.gov (United States)

    Reis, Rodrigo S; Eamens, Andrew L; Roberts, Thomas H; Waterhouse, Peter M

    2015-01-01

    In Arabidopsis thaliana, efficient microRNA (miRNA) production requires DICER-LIKE1 (DCL1) with the assistance of a partnering protein, DOUBLE-STRANDED RNA BINDING1 (DRB1) or DRB2. The presence of either of these DRB proteins is crucial to determine the mode of action of a miRNA; i.e., cleavage or translation inhibition. Here we studied the structural determinants for the role of DRB1 and DRB2 in the miRNA pathway. We developed a series of chimeric vectors encoding different functional domains of DRB1 and DRB2, and expressed these in the drb1 mutant background in Arabidopsis under the control of the native DRB1 promoter. Complementation of the drb1 developmental phenotype was used to assess the biological role that each functional domain of DRB1 and DRB2 mediates in the miRNA-guided transcript cleavage pathway. The DRB1 amino acid sequence differs considerably to that of DRB2, and analysis of drb1 transgenic lines revealed that the first dsRNA-binding domains of DRB1 and DRB2 are functionally similar; in contrast, the dsRBD2 of DRB1 and DRB2 appear functionally distinct. Our bioinformatic analysis further suggests that the C-terminal domain of DRB2 mediates a functional role in the miRNA pathway, whereas its counterpart in DRB1 is known to be dispensable. Our results provide evidence for the differences between DRB1 and DRB2 proteins in vivo, which may be essential for the selection of the miRNA regulatory mechanisms, and suggest that these features are conserved among land plants.

  7. SUMOylation of GTF2IRD1 regulates protein partner interactions and ubiquitin-mediated degradation.

    Directory of Open Access Journals (Sweden)

    Jocelyn Widagdo

    Full Text Available GTF2IRD1 is one of the genes implicated in Williams-Beuren syndrome, a disease caused by haploinsufficiency of certain dosage-sensitive genes within a hemizygous microdeletion of chromosome 7. GTF2IRD1 is a prime candidate for some of the major features of the disease, presumably caused by abnormally reduced abundance of this putative transcriptional repressor protein. GTF2IRD1 has been shown to interact with the E3 SUMO ligase PIASxβ, but the significance of this relationship is largely unexplored. Here, we demonstrate that GTF2IRD1 can be SUMOylated by the SUMO E2 ligase UBC9 and the level of SUMOylation is enhanced by PIASxβ. A major SUMOylation site was mapped to lysine 495 within a conserved SUMO consensus motif. SUMOylation of GTF2IRD1 alters the affinity of the protein for binding partners that contain SUMO-interacting motifs, including a novel family member of the HDAC repressor complex, ZMYM5, and PIASxβ itself. In addition, we show that GTF2IRD1 is targeted for ubiquitination and proteasomal degradation. Cross regulation by SUMOylation modulates this process, thus potentially regulating the level of GTF2IRD1 protein in the cell. These findings, concerning post-translational control over the activity and stability of GTF2IRD1, together with previous work showing how GTF2IRD1 directly regulates its own transcription levels suggest an evolutionary requirement for fine control over GTF2IRD1 activity in the cell.

  8. The fusion partner specifies the oncogenic potential of NUP98 fusion proteins

    OpenAIRE

    Saw, Jesslyn; Curtis, David J.; Hussey, Damian J.; Dobrovic, Alexander; Aplan, Peter D.; Slape, Christopher I.

    2013-01-01

    NUP98 is among the most promiscuously translocated genes in hematological diseases. Among the 28 known fusion partners, there are two categories: homeobox genes and non-homeobox genes. The homeobox fusion partners are well-studied in animal models, resulting in HoxA cluster overexpression and hematological disease. The non- homeobox fusion partners are less well studied. We created transgenic animal models for three NUP98 fusion genes (one homeobox, two non- homeobox), and show that in this s...

  9. The calcium-binding protein of Entamoeba histolytica as a fusion partner for expression of peptides in Escherichia coli.

    Science.gov (United States)

    Reddi, Honey; Bhattacharya, Alok; Kumar, Vijay

    2002-12-01

    We describe the construction of an Escherichia coli expression vector, CBP that allows the C-terminal fusion of heterologous proteins to the calcium-binding protein (CaBP) of the parasitic protozoan Entamoeba histolytica. The intrinsic nature of this protein to remain soluble on heat treatment has been exploited in its use as a novel fusion partner. The presence of a histidine tag and an enterokinase recognition site, aid in the affinity purification and proteolytic cleavage of the fusion protein. The efficacy of the vector was tested using the preS1 region of the envelope protein of the hepatitis B virus. The CaBP-preS1 fusion protein partitioned in the soluble fraction on heat treatment and this facilitated its rapid purification.

  10. Merging in-silico and in vitro salivary protein complex partners using the STRING database: A tutorial.

    Science.gov (United States)

    Crosara, Karla Tonelli Bicalho; Moffa, Eduardo Buozi; Xiao, Yizhi; Siqueira, Walter Luiz

    2018-01-16

    Protein-protein interaction is a common physiological mechanism for protection and actions of proteins in an organism. The identification and characterization of protein-protein interactions in different organisms is necessary to better understand their physiology and to determine their efficacy. In a previous in vitro study using mass spectrometry, we identified 43 proteins that interact with histatin 1. Six previously documented interactors were confirmed and 37 novel partners were identified. In this tutorial, we aimed to demonstrate the usefulness of the STRING database for studying protein-protein interactions. We used an in-silico approach along with the STRING database (http://string-db.org/) and successfully performed a fast simulation of a novel constructed histatin 1 protein-protein network, including both the previously known and the predicted interactors, along with our newly identified interactors. Our study highlights the advantages and importance of applying bioinformatics tools to merge in-silico tactics with experimental in vitro findings for rapid advancement of our knowledge about protein-protein interactions. Our findings also indicate that bioinformatics tools such as the STRING protein network database can help predict potential interactions between proteins and thus serve as a guide for future steps in our exploration of the Human Interactome. Our study highlights the usefulness of the STRING protein database for studying protein-protein interactions. The STRING database can collect and integrate data about known and predicted protein-protein associations from many organisms, including both direct (physical) and indirect (functional) interactions, in an easy-to-use interface. Copyright © 2017 Elsevier B.V. All rights reserved.

  11. TOX4 and NOVA1 proteins are partners of the LEDGF PWWP domain and affect HIV-1 replication.

    Directory of Open Access Journals (Sweden)

    Mehdi Morchikh

    Full Text Available PWWP domains are involved in the chromatin attachment of several proteins. They bind to both DNA and proteins and their interaction with specific histone methylation marks define them as a new class of histone code readers. The lens epithelium derived growth factor (LEDGF/p75 contains an N-terminal PWWP domain necessary for its interaction with chromatin but also a C-terminal domain which interacts with several proteins, such as lentiviral integrases. These two domains confer a chromatin-tethering function to LEDGF/p75 and in the case of lentiviral integrases, this tethering participates in the efficiency and site selectivity of integration. Although proteins interacting with LEDGF/p75 C-terminal domain have been extensively studied, no data exist about partners of its PWWP domain regulating its interaction with chromatin. In this study, we report the identification by yeast-two-hybrid of thirteen potential partners of the LEDGF PWWP domain. Five of these interactions were confirmed in mammalian cells, using both a protein complementation assay and co-immunoprecipitation approaches. Three of these partners interact with full length LEDGF/p75, they are specific for PWWP domains of the HDGF family and they require PWWP amino acids essential for the interaction with chromatin. Among them, the transcription activator TOX4 and the splicing cofactor NOVA1 were selected for a more extensive study. These two proteins or their PWWP interacting regions (PIR colocalize with LEDGF/p75 in Hela cells and interact in vitro in the presence of DNA. Finally, single round VSV-G pseudotyped HIV-1 but not MLV infection is inhibited in cells overexpressing these two PIRs. The observed inhibition of infection can be attributed to a defect in the integration step. Our data suggest that a regulation of LEDGF interaction with chromatin by cellular partners of its PWWP domain could be involved in several processes linked to LEDGF tethering properties, such as lentiviral

  12. The Deinococcus radiodurans DR1245 Protein, a DdrB Partner Homologous to YbjN Proteins and Reminiscent of Type III Secretion System Chaperones

    Science.gov (United States)

    Bouthier-de-la-Tour, Claire; Coureux, Pierre-Damien; Ithurbide, Solenne; Vannier, Françoise; Guerin, Philippe P.; Dulberger, Charles L.; Satyshur, Kenneth A.; Keck, James L.; Armengaud, Jean; Cox, Michael M.; Sommer, Suzanne

    2013-01-01

    The bacterium Deinococcus radiodurans exhibits an extreme resistance to ionizing radiation. A small subset of Deinococcus genus-specific genes were shown to be up-regulated upon exposure to ionizing radiation and to play a role in genome reconstitution. These genes include an SSB-like protein called DdrB. Here, we identified a novel protein encoded by the dr1245 gene as an interacting partner of DdrB. A strain devoid of the DR1245 protein is impaired in growth, exhibiting a generation time approximately threefold that of the wild type strain while radioresistance is not affected. We determined the three-dimensional structure of DR1245, revealing a relationship with type III secretion system chaperones and YbjN family proteins. Thus, DR1245 may display some chaperone activity towards DdrB and possibly other substrates. PMID:23441204

  13. The Deinococcus radiodurans DR1245 Protein, a DdrB Partner Homologous to YbjN Proteins and Reminiscent of Type III Secretion System Chaperones

    Energy Technology Data Exchange (ETDEWEB)

    Norais, Cédric [Univ. of Wisconsin, Madison, WI (United States); Ecole Polytechnic, Paaiseau (France); Servant, Pascale [Univ. of Paris-Sud, Orsay (France); National Center of Scientific Research, Orsay (France); Bouthier-de-la-Tour, Claire [Univ. of Paris-Sud, Orsay (France); National Center of Scientific Research, Orsay (France); Coureux, Pierre-Damien [Ecole Polytechnic, Paaiseau (France); Ithurbide, Solenne [Univ. of Paris-Sud, Orsay (France); National Center of Scientific Research, Orsay (France); Vannier, Françoise [Univ. of Paris-Sud, Orsay (France); National Center of Scientific Research, Orsay (France); Guerin, Philippe P. [French Alternatives Energy Commission (France); Dulberger, Charles L. [Univ. of Wisconsin, Madison, WI (United States); Satyshur, Kenneth A. [Univ. of Wisconsin, Madison, WI (United States); Keck, James L. [Univ. of Wisconsin, Madison, WI (United States); Armengaud, Jean [French Alternatives Energy Commission (France); Cox, Michael M. [Univ. of Wisconsin, Madison, WI (United States); Sommer, Suzanne [Univ. of Paris-Sud, Orsay (France); National Center of Scientific Research, Orsay (France)

    2013-02-18

    The bacterium Deinococcus radiodurans exhibits an extreme resistance to ionizing radiation. A small subset of Deinococcus genus-specific genes were shown to be up-regulated upon exposure to ionizing radiation and to play a role in genome reconstitution. These genes include an SSB-like protein called DdrB. Here, we identified a novel protein encoded by the dr1245gene as an interacting partner of DdrB. A strain devoid of the DR1245 protein is impaired in growth, exhibiting a generation time approximately threefold that of the wild type strain while radioresistance is not affected. We determined the three-dimensional structure of DR1245, revealing a relationship with type III secretion system chaperones and YbjN family proteins. Thus, DR1245 may display some chaperone activity towards DdrB and possibly other substrates.

  14. The Deinococcus radiodurans DR1245 protein, a DdrB partner homologous to YbjN proteins and reminiscent of type III secretion system chaperones.

    Directory of Open Access Journals (Sweden)

    Cédric Norais

    Full Text Available The bacterium Deinococcus radiodurans exhibits an extreme resistance to ionizing radiation. A small subset of Deinococcus genus-specific genes were shown to be up-regulated upon exposure to ionizing radiation and to play a role in genome reconstitution. These genes include an SSB-like protein called DdrB. Here, we identified a novel protein encoded by the dr1245 gene as an interacting partner of DdrB. A strain devoid of the DR1245 protein is impaired in growth, exhibiting a generation time approximately threefold that of the wild type strain while radioresistance is not affected. We determined the three-dimensional structure of DR1245, revealing a relationship with type III secretion system chaperones and YbjN family proteins. Thus, DR1245 may display some chaperone activity towards DdrB and possibly other substrates.

  15. Transient interactions studied by NMR : iron sulfur proteins and their interaction partners

    NARCIS (Netherlands)

    Xu, Xingfu

    2009-01-01

    The interactions between proteins are of central importance for virtually every process in a living cell. It has long been a mystery how two proteins associate to form a complex in a complicated cellular context. Recently, it was found that an intermediate state called encounter state, of a protein

  16. Accessibility and partner number of protein residues, their relationship and a webserver, ContPlot for their display

    Directory of Open Access Journals (Sweden)

    Ray Partha

    2009-04-01

    Full Text Available Abstract Background Depending on chemical features residues have preferred locations – interior or exterior – in protein structures, which also determine how many other residues are found around them. The close packing of residues is the hallmark of protein interior and protein-protein interaction sites. Results The average values of accessible surface area (ASA and partner number (PN, the number of other residues within a distance of 4.5 Å from any atom of a given residue of different residues have been determined and a webserver, ContPlot has been designed to display these values (relative to the average values along the protein sequence. This would be useful to visually identify residues that are densely packed, or those involved in protein-protein interactions. The skewness observed in the distribution of PNs is indicative of the hydrophobic or hydrophilic nature of the residue. The variation of ASA with PN can be analytically expressed in terms of a cubic equation. These equations (one for each residue can be used to estimate the ASA of a polypeptide chain using the PNs of the individual residues in the structure. Conclusion The atom-based PNs (obtained by counting surrounding atoms are highly correlated to the residue-based PN, indicating that the latter can adequately capture the atomic details of packing. The average values of ASA and PN associated with each residue should be useful in protein structure prediction or fold-recognition algorithm. ContPlot would provide a handy tool to assess the importance of a residue in the protein structure or interaction site.

  17. Multiple interaction partners for Cockayne syndrome proteins: implications for genome and transcriptome maintenance

    Science.gov (United States)

    Aamann, Maria D.; Muftuoglu, Meltem; Bohr, Vilhelm A.; Stevnsner, Tinna

    2013-01-01

    Cockayne syndrome (CS) is characterized by progressive multisystem degeneration and is classified as a segmental premature aging syndrome. The majority of CS cases are caused by defects in the CS complementation group B (CSB) protein and the rest are mainly caused by defects in the CS complementation group A (CSA) protein. Cells from CS patients are sensitive to UV light and a number of other DNA damaging agents including various types of oxidative stress. The cells also display transcription deficiencies, abnormal apoptotic response to DNA damage, and DNA repair deficiencies. Herein we have critically reviewed the current knowledge about known protein interactions of the CS proteins. The review focuses on the participation of the CSB and CSA proteins in many different protein interactions and complexes, and how these interactions inform us about pathways that are defective in the disease. PMID:23583689

  18. BCL-2 family proteins: changing partners in the dance towards death.

    Science.gov (United States)

    Kale, Justin; Osterlund, Elizabeth J; Andrews, David W

    2018-01-01

    The BCL-2 family of proteins controls cell death primarily by direct binding interactions that regulate mitochondrial outer membrane permeabilization (MOMP) leading to the irreversible release of intermembrane space proteins, subsequent caspase activation and apoptosis. The affinities and relative abundance of the BCL-2 family proteins dictate the predominate interactions between anti-apoptotic and pro-apoptotic BCL-2 family proteins that regulate MOMP. We highlight the core mechanisms of BCL-2 family regulation of MOMP with an emphasis on how the interactions between the BCL-2 family proteins govern cell fate. We address the critical importance of both the concentration and affinities of BCL-2 family proteins and show how differences in either can greatly change the outcome. Further, we explain the importance of using full-length BCL-2 family proteins (versus truncated versions or peptides) to parse out the core mechanisms of MOMP regulation by the BCL-2 family. Finally, we discuss how post-translational modifications and differing intracellular localizations alter the mechanisms of apoptosis regulation by BCL-2 family proteins. Successful therapeutic intervention of MOMP regulation in human disease requires an understanding of the factors that mediate the major binding interactions between BCL-2 family proteins in cells.

  19. Identifying diabetes-related important protein targets with few interacting partners with the PageRank algorithm.

    Science.gov (United States)

    Grolmusz, Vince I

    2015-04-01

    Diabetes is a growing concern for the developed nations worldwide. New genomic, metagenomic and gene-technologic approaches may yield considerable results in the next several years in its early diagnosis, or in advances in therapy and management. In this work, we highlight some human proteins that may serve as new targets in the early diagnosis and therapy. With the help of a very successful mathematical tool for network analysis that formed the basis of the early successes of Google(TM), Inc., we analyse the human protein-protein interaction network gained from the IntAct database with a mathematical algorithm. The novelty of our approach is that the new protein targets suggested do not have many interacting partners (so, they are not hubs or super-hubs), so their inhibition or promotion probably will not have serious side effects. We have identified numerous possible protein targets for diabetes therapy and/or management; some of these have been well known for a long time (these validate our method), some of them appeared in the literature in the last 12 months (these show the cutting edge of the algorithm), and the remainder are still unknown to be connected with diabetes, witnessing completely new hits of the method.

  20. Characterization of the Structure and Intermolecular Interactions between the Connexin 32 Carboxyl-terminal Domain and the Protein Partners Synapse-associated Protein 97 and Calmodulin*

    Science.gov (United States)

    Stauch, Kelly; Kieken, Fabien; Sorgen, Paul

    2012-01-01

    In Schwann cells, connexin 32 (Cx32) can oligomerize to form intracellular gap junction channels facilitating a shorter pathway for metabolite diffusion across the layers of the myelin sheath. The mechanisms of Cx32 intracellular channel regulation have not been clearly defined. However, Ca2+, pH, and the phosphorylation state can regulate Cx32 gap junction channels, in addition to the direct interaction of protein partners with the carboxyl-terminal (CT) domain. In this study, we used different biophysical methods to determine the structure and characterize the interaction of the Cx32CT domain with the protein partners synapse-associated protein 97 (SAP97) and calmodulin (CaM). Our results revealed that the Cx32CT is an intrinsically disordered protein that becomes α-helical upon binding CaM. We identified the GUK domain as the minimal SAP97 region necessary for the Cx32CT interaction. The Cx32CT residues affected by the binding of CaM and the SAP97 GUK domain were determined as well as the dissociation constants for these interactions. We characterized three Cx32CT Charcot-Marie-Tooth disease mutants (R219H, R230C, and F235C) and identified that whereas they all formed functional channels, they all showed reduced binding affinity for SAP97 and CaM. Additionally, we report that in RT4-D6P2T rat schwannoma cells, Cx32 is differentially phosphorylated and exists in a complex with SAP97 and CaM. Our studies support the importance of protein-protein interactions in the regulation of Cx32 gap junction channels and myelin homeostasis. PMID:22718765

  1. Protein interacting with C kinase 1 (PICK1) reduces reinsertion rates of interaction partners sorted to Rab11-dependent slow recycling pathway

    DEFF Research Database (Denmark)

    Madsen, Kenneth Lindegaard; Thorsen, Thor Seneca; Rahbek-Clemmensen, Troels

    2012-01-01

    The scaffolding protein PICK1 (protein interacting with C kinase 1) contains an N-terminal PSD-95/Discs large/ZO-1 (PDZ) domain and a central lipid-binding Bin/amphiphysin/Rvs (BAR) domain. PICK1 is thought to regulate trafficking of its PDZ binding partners but different and even opposing...

  2. A data-mining approach to rank candidate protein-binding partners-The case of biogenesis of lysosome-related organelles complex-1 (BLOC-1).

    Science.gov (United States)

    Rodriguez-Fernandez, I A; Dell'Angelica, E C

    2009-04-01

    The study of protein-protein interactions is a powerful approach to uncovering the molecular function of gene products associated with human disease. Protein-protein interaction data are accumulating at an unprecedented pace owing to interactomics projects, although it has been recognized that a significant fraction of these data likely represents false positives. During our studies of biogenesis of lysosome-related organelles complex-1 (BLOC-1), a protein complex involved in protein trafficking and containing the products of genes mutated in Hermansky-Pudlak syndrome, we faced the problem of having too many candidate binding partners to pursue experimentally. In this work, we have explored ways of efficiently gathering high-quality information about candidate binding partners and presenting the information in a visually friendly manner. We applied the approach to rank 70 candidate binding partners of human BLOC-1 and 102 candidates of its counterpart from Drosophila melanogaster. The top candidate for human BLOC-1 was the small GTPase encoded by the RAB11A gene, which is a paralogue of the Rab38 and Rab32 proteins in mammals and the lightoid gene product in flies. Interestingly, genetic analyses in D. melanogaster uncovered a synthetic sick/lethal interaction between Rab11 and lightoid. The data-mining approach described herein can be customized to study candidate binding partners for other proteins or possibly candidates derived from other types of 'omics' data.

  3. Phloem protein partners of Cucurbit aphid borne yellows virus: possible involvement of phloem proteins in virus transmission by aphids.

    Science.gov (United States)

    Bencharki, B; Boissinot, S; Revollon, S; Ziegler-Graff, V; Erdinger, M; Wiss, L; Dinant, S; Renard, D; Beuve, M; Lemaitre-Guillier, C; Brault, V

    2010-06-01

    Poleroviruses are phytoviruses strictly transmitted by phloem-feeding aphids in a circulative and nonpropagative mode. During ingestion, aphids sample virions in sieve tubes along with sap. Therefore, any sap protein bound to virions will be acquired by the insects and could potentially be involved in the transmission process. By developing in vitro virus-overlay assays on sap proteins collected from cucumber, we observed that approximately 20 proteins were able to bind to purified particles of Cucurbit aphid borne yellows virus (CABYV). Among them, eight proteins were identified by mass spectrometry. The role of two candidates belonging to the PP2-like family (predominant lectins found in cucurbit sap) in aphid transmission was further pursued by using purified orthologous PP2 proteins from Arabidopsis. Addition of these proteins to the virus suspension in the aphid artificial diet greatly increased virus transmission rate. This shift was correlated with an increase in the number of viral genomes in insect cells and with an increase of virion stability in vitro. Surprisingly, increase of the virus transmission rate was also monitored after addition of unrelated proteins in the aphid diet, suggesting that any soluble protein at sufficiently high concentration in the diet and acquired together with virions could stimulate virus transmission.

  4. FEZ2 has acquired additional protein interaction partners relative to FEZ1: functional and evolutionary implications.

    Science.gov (United States)

    Alborghetti, Marcos R; Furlan, Ariane S; Kobarg, Jörg

    2011-03-08

    The FEZ (fasciculation and elongation protein zeta) family designation was purposed by Bloom and Horvitz by genetic analysis of C. elegans unc-76. Similar human sequences were identified in the expressed sequence tag database as FEZ1 and FEZ2. The unc-76 function is necessary for normal axon fasciculation and is required for axon-axon interactions. Indeed, the loss of UNC-76 function results in defects in axonal transport. The human FEZ1 protein has been shown to rescue defects caused by unc-76 mutations in nematodes, indicating that both UNC-76 and FEZ1 are evolutionarily conserved in their function. Until today, little is known about FEZ2 protein function. Using the yeast two-hybrid system we demonstrate here conserved evolutionary features among orthologs and non-conserved features between paralogs of the FEZ family of proteins, by comparing the interactome profiles of the C-terminals of human FEZ1, FEZ2 and UNC-76 from C. elegans. Furthermore, we correlate our data with an analysis of the molecular evolution of the FEZ protein family in the animal kingdom. We found that FEZ2 interacted with 59 proteins and that of these only 40 interacted with FEZ1. Of the 40 FEZ1 interacting proteins, 36 (90%), also interacted with UNC-76 and none of the 19 FEZ2 specific proteins interacted with FEZ1 or UNC-76. This together with the duplication of unc-76 gene in the ancestral line of chordates suggests that FEZ2 is in the process of acquiring new additional functions. The results provide also an explanation for the dramatic difference between C. elegans and D. melanogaster unc-76 mutants on one hand, which cause serious defects in the nervous system, and the mouse FEZ1 -/- knockout mice on the other, which show no morphological and no strong behavioural phenotype. Likely, the ubiquitously expressed FEZ2 can completely compensate the lack of neuronal FEZ1, since it can interact with all FEZ1 interacting proteins and additional 19 proteins.

  5. High Mobility Group B Proteins, Their Partners, and Other Redox Sensors in Ovarian and Prostate Cancer

    Directory of Open Access Journals (Sweden)

    Aida Barreiro-Alonso

    2016-01-01

    Full Text Available Cancer cells try to avoid the overproduction of reactive oxygen species by metabolic rearrangements. These cells also develop specific strategies to increase ROS resistance and to express the enzymatic activities necessary for ROS detoxification. Oxidative stress produces DNA damage and also induces responses, which could help the cell to restore the initial equilibrium. But if this is not possible, oxidative stress finally activates signals that will lead to cell death. High mobility group B (HMGB proteins have been previously related to the onset and progressions of cancers of different origins. The protein HMGB1 behaves as a redox sensor and its structural changes, which are conditioned by the oxidative environment, are associated with different functions of the protein. This review describes recent advances in the role of human HMGB proteins and other proteins interacting with them, in cancerous processes related to oxidative stress, with special reference to ovarian and prostate cancer. Their participation in the molecular mechanisms of resistance to cisplatin, a drug commonly used in chemotherapy, is also revised.

  6. DEF- and GLO-like proteins may have lost most of their interaction partners during angiosperm evolution.

    Science.gov (United States)

    Melzer, Rainer; Härter, Andrea; Rümpler, Florian; Kim, Sangtae; Soltis, Pamela S; Soltis, Douglas E; Theißen, Günter

    2014-11-01

    early-diverging angiosperms may be one reason for the highly diverse flower morphologies observed in these species. The results strengthen the hypothesis that a reduction in the number of interaction partners of DEF- and GLO-like proteins, with DEF-GLO heterodimers remaining the only DNA-binding dimers in core eudicots, contributed to developmental robustness, canalization of flower development and the diversification of angiosperms. © The Author 2014. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  7. Searching for cellular partners of hantaviral nonstructural protein NSs: Y2H screening of mouse cDNA library and analysis of cellular interactome.

    Directory of Open Access Journals (Sweden)

    Tuomas Rönnberg

    Full Text Available Hantaviruses (Bunyaviridae are negative-strand RNA viruses with a tripartite genome. The small (S segment encodes the nucleocapsid protein and, in some hantaviruses, also the nonstructural protein (NSs. The aim of this study was to find potential cellular partners for the hantaviral NSs protein. Toward this aim, yeast two-hybrid (Y2H screening of mouse cDNA library was performed followed by a search for potential NSs protein counterparts via analyzing a cellular interactome. The resulting interaction network was shown to form logical, clustered structures. Furthermore, several potential binding partners for the NSs protein, for instance ACBD3, were identified and, to prove the principle, interaction between NSs and ACBD3 proteins was demonstrated biochemically.

  8. Expression and modulation of translocator protein and its partners by hypoxia reoxygenation or ischemia and reperfusion in porcine renal models

    Science.gov (United States)

    Favreau, Frederic; Rossard, Ludivine; Zhang, Keqiang; Desurmont, Thibault; Manguy, Emilie; Belliard, Aude; Fabre, Stéphane; Liu, Jun; Han, Zeqiu; Thuillier, Raphael; Papadopoulos, Vassilios; Hauet, Thierry

    2009-01-01

    Translocator protein (TSPO), formerly known as the peripheral-type benzodiazepine receptor, is an 18-kDa drug- and cholesterol-binding protein localized to the outer mitochondrial membrane and implicated in a variety of cell and mitochondrial functions. To determine the role of TSPO in ischemia-reperfusion injury (IRI), we used both in vivo and in vitro porcine models: an in vivo renal ischemia model where different conservation modalities were tested and an in vitro model where TSPO-transfected porcine proximal tubule LLC-PK1 cells were exposed to hypoxia and oxidative stress. The expression of TSPO and its partners in steroidogenic cells, steroidogenic acute regulatory protein (StAR) and cytochrome P-450 side chain cleavage CYP11A1, as well as the impact of TSPO overexpression and exposure to TSPO ligands in vitro in hypoxia-ischemia conditions were investigated. Hypoxia induced caspase activation, reduction of ATP content, and LLC-PK1 cell death. Transfection and overexpression of TSPO rescued the cells from the detrimental effects of hypoxia and reoxygenation. Moreover, TSPO overexpression was accompanied by a reduction of H2O2-induced necrosis. TSPO drug ligands did not affect TSPO-mediated functions. In vivo, TSPO expression was modulated by IRI and during regeneration particularly in proximal tubule cells, which do not express this protein at the basal level. Under the same conditions, StAR and CYP11A1 protein and gene expression was reduced without apparent relation to TSPO changes. Pregnenolone was identified and measured in the pig kidney. Pregnenolone synthesis was not affected by the experimental conditions used. Taken together, these results indicate that changes in TSPO expression in kidney regenerating tissue could be important for renal protection and maintenance of kidney function. PMID:19386723

  9. Phosphorylation status of the Kep1 protein alters its affinity for its protein binding partner alternative splicing factor ASF/SF2.

    Science.gov (United States)

    Robard, Cécile; Daviau, Alex; Di Fruscio, Marco

    2006-11-15

    Mutations in the Drosophila kep1 gene, encoding a single maxi KH (K homology) domain-containing RNA-binding protein, result in a reduction of fertility in part due to the disruption of the apoptotic programme during oogenesis. This disruption is concomitant with the appearance of an alternatively spliced mRNA isoform encoding the inactive caspase dredd. We generated a Kep1 antibody and have found that the Kep1 protein is present in the nuclei of both the follicle and nurse cells during all stages of Drosophila oogenesis. We have shown that the Kep1 protein is phosphorylated in ovaries induced to undergo apoptosis following treatment with the topoisomerase I inhibitor camptothecin. We have also found that the Kep1 protein interacts specifically with the SR (serine/arginine-rich) protein family member ASF/SF2 (alternative splicing factor/splicing factor 2). This interaction is independent of the ability of Kep1 to bind RNA, but is dependent on the phosphorylation of the Kep1 protein, with the interaction between Kep1 and ASF/SF2 increasing in the presence of activated Src. Using a CD44v5 alternative splicing reporter construct, we observed 99% inclusion of the alternatively spliced exon 5 following kep1 transfection in a cell line that constitutively expresses activated Src. This modulation in splicing was not observed in the parental NIH 3T3 cell line in which we obtained 7.5% exon 5 inclusion following kep1 transfection. Our data suggest a mechanism of action in which the in vivo phosphorylation status of the Kep1 protein affects its affinity towards its protein binding partners and in turn may allow for the modulation of alternative splice site selection in Kep1-ASF/SF2-dependent target genes.

  10. Evaluation of chemical fluorescent dyes as a protein conjugation partner for live cell imaging.

    Directory of Open Access Journals (Sweden)

    Yoko Hayashi-Takanaka

    Full Text Available To optimize live cell fluorescence imaging, the choice of fluorescent substrate is a critical factor. Although genetically encoded fluorescent proteins have been used widely, chemical fluorescent dyes are still useful when conjugated to proteins or ligands. However, little information is available for the suitability of different fluorescent dyes for live imaging. We here systematically analyzed the property of a number of commercial fluorescent dyes when conjugated with antigen-binding (Fab fragments directed against specific histone modifications, in particular, phosphorylated H3S28 (H3S28ph and acetylated H3K9 (H3K9ac. These Fab fragments were conjugated with a fluorescent dye and loaded into living HeLa cells. H3S28ph-specific Fab fragments were expected to be enriched in condensed chromosomes, as H3S28 is phosphorylated during mitosis. However, the degree of Fab fragment enrichment on mitotic chromosomes varied depending on the conjugated dye. In general, green fluorescent dyes showed higher enrichment, compared to red and far-red fluorescent dyes, even when dye:protein conjugation ratios were similar. These differences are partly explained by an altered affinity of Fab fragment after dye-conjugation; some dyes have less effect on the affinity, while others can affect it more. Moreover, red and far-red fluorescent dyes tended to form aggregates in the cytoplasm. Similar results were observed when H3K9ac-specific Fab fragments were used, suggesting that the properties of each dye affect different Fab fragments similarly. According to our analysis, conjugation with green fluorescent dyes, like Alexa Fluor 488 and Dylight 488, has the least effect on Fab affinity and is the best for live cell imaging, although these dyes are less photostable than red fluorescent dyes. When multicolor imaging is required, we recommend the following dye combinations for optimal results: Alexa Fluor 488 (green, Cy3 (red, and Cy5 or CF640 (far-red.

  11. Interactions of ataxin-3 with its molecular partners in the protein machinery that sorts protein aggregates to the aggresome.

    Science.gov (United States)

    Bonanomi, Marcella; Mazzucchelli, Serena; D'Urzo, Annalisa; Nardini, Marco; Konarev, Petr V; Invernizzi, Gaetano; Svergun, Dmitri I; Vanoni, Marco; Regonesi, Maria Elena; Tortora, Paolo

    2014-06-01

    Ataxin-3 (AT3) is the protein that triggers the inherited neurodegenerative disorder spinocerebellar ataxia type 3 when its polyglutamine (polyQ) stretch close to the C-terminus exceeds a critical length. AT3 consists of the N-terminal globular Josephin domain (JD) and the C-terminal disordered one. It cleaves isopeptide bonds between ubiquitin monomers, an event involved in protein quality control mechanisms. AT3 has been implicated in the pathway that sorts aggregated protein to aggresomes via microtubules, in which dynein and histone deacetylase 6 (HDAC6) also seem to be involved. By taking advantage of small angle X-ray scattering (SAXS) and surface plasmon resonance (SPR), we have investigated the interaction of AT3 with tubulin and HDAC6. Based on SAXS results, the AT3 oligomer, consisting of 6-7 subunits, tightly binds to the tubulin hexameric oligomer in a "parallel" fashion. By SPR analysis we have demonstrated that AT3 binds to tubulin dimer with a 50nM affinity. Binding fits with a Langmuir 1:1 model and involves a single binding interface. Nevertheless, the interaction surface consists of three distinct, discontinuous tubulin-binding regions (TBR), one located in the JD, and the two others in the disordered domain, upstream and downstream of the polyQ stretch. In the absence of any of the three TBRs, the affinity is drastically reduced. By SPR we have also provided the first evidence of direct binding of AT3 to HDAC6, with affinity in the range 0.1-1μM. These results shed light on the interactions among the components of the transport machinery that sorts aggregate protein to the aggresome, and pave the way to in vivo studies aimed at further clarifying their roles. Copyright © 2014 Elsevier Ltd. All rights reserved.

  12. Liver-specific ZP domain-containing protein (LZP) as a new partner of Tamm-Horsfall protein harbors on renal tubules.

    Science.gov (United States)

    Shen, Hai-Lian; Xu, Zhi-Gang; Huang, Li-Yu; Liu, Dong; Lin, Dao-Hong; Cao, Jia-Bin; Zhang, Xin; Wang, Zhi-Qin; Wang, Wen-Hui; Yang, Peng-Yuan; Han, Ze-Guang

    2009-01-01

    Liver-specific ZP domain-containing protein (LZP) was recently identified as a secreted protein that is specifically expressed in liver. However, the physiological effects of LZP are largely unknown. In this study, we found that LZP was detectable in mouse kidneys, testes, ovaries and heart, in addition to liver. LZP was localized in the spermatid cells of testes, corpus luteum cells of ovaries, and cardiac muscle cells of heart. But the protein mainly anchored on the apical membrane of the thick ascending limb of the loop of Henle (TAL) cell in mouse kidney. In rat kidney LZP and Tamm-Horsfall protein (THP) were co-localized in TAL. The in vivo interaction between LZP and THP was confirmed in kidney and urine by co-immunoprecipitation assay, and the in vitro interaction was detected by GST pull-down assay, implying that the interaction could be independent on N-linked glycosylated modification of LZP. Surprisingly, LZPs with intramolecular disulfide bridges could self-interact, and then self-aggregate into spheres of varying sizes, but not polymerize into filaments. The finding that LZP might act as a new partner of THP would provide novel insights into renal functions related to THP and LZP, such as the urothelial permeability barrier and the host defense against the adhesion of pathogens.

  13. Protein Kinase CK2: A Targetable BCR-ABL Partner in Philadelphia Positive Leukemias

    Directory of Open Access Journals (Sweden)

    Alessandro Morotti

    2015-01-01

    Full Text Available BCR-ABL-mediated leukemias, either Chronic Myeloid Leukemia (CML or Philadelphia positive Acute Lymphoblastic Leukemia (ALL, are the paradigm of targeted molecular therapy of cancer due to the impressive clinical responses obtained with BCR-ABL specific tyrosine kinase inhibitors (TKIs. However, BCR-ABL TKIs do not allow completely eradicating both CML and ALL. Furthermore, ALL therapy is associated with much worse responses to TKIs than those observed in CML. The identification of additional pathways that mediate BCR-ABL leukemogenesis is indeed mandatory to achieve synthetic lethality together with TKI. Here, we review the role of BCR-ABL/protein kinase CK2 interaction in BCR-ABL leukemias, with potentially relevant implications for therapy.

  14. TRF1 and TRF2 use different mechanisms to find telomeric DNA but share a novel mechanism to search for protein partners at telomeres.

    Science.gov (United States)

    Lin, Jiangguo; Countryman, Preston; Buncher, Noah; Kaur, Parminder; E, Longjiang; Zhang, Yiyun; Gibson, Greg; You, Changjiang; Watkins, Simon C; Piehler, Jacob; Opresko, Patricia L; Kad, Neil M; Wang, Hong

    2014-02-01

    Human telomeres are maintained by the shelterin protein complex in which TRF1 and TRF2 bind directly to duplex telomeric DNA. How these proteins find telomeric sequences among a genome of billions of base pairs and how they find protein partners to form the shelterin complex remains uncertain. Using single-molecule fluorescence imaging of quantum dot-labeled TRF1 and TRF2, we study how these proteins locate TTAGGG repeats on DNA tightropes. By virtue of its basic domain TRF2 performs an extensive 1D search on nontelomeric DNA, whereas TRF1's 1D search is limited. Unlike the stable and static associations observed for other proteins at specific binding sites, TRF proteins possess reduced binding stability marked by transient binding (∼ 9-17 s) and slow 1D diffusion on specific telomeric regions. These slow diffusion constants yield activation energy barriers to sliding ∼ 2.8-3.6 κ(B)T greater than those for nontelomeric DNA. We propose that the TRF proteins use 1D sliding to find protein partners and assemble the shelterin complex, which in turn stabilizes the interaction with specific telomeric DNA. This 'tag-team proofreading' represents a more general mechanism to ensure a specific set of proteins interact with each other on long repetitive specific DNA sequences without requiring external energy sources.

  15. A Kinase Anchoring Protein 9 Is a Novel Myosin VI Binding Partner That Links Myosin VI with the PKA Pathway in Myogenic Cells

    Directory of Open Access Journals (Sweden)

    Justyna Karolczak

    2015-01-01

    Full Text Available Myosin VI (MVI is a unique motor protein moving towards the minus end of actin filaments unlike other known myosins. Its important role has recently been postulated for striated muscle and myogenic cells. Since MVI functions through interactions of C-terminal globular tail (GT domain with tissue specific partners, we performed a search for MVI partners in myoblasts and myotubes using affinity chromatography with GST-tagged MVI-GT domain as a bait. A kinase anchoring protein 9 (AKAP9, a regulator of PKA activity, was identified by means of mass spectrometry as a possible MVI interacting partner both in undifferentiated and differentiating myoblasts and in myotubes. Coimmunoprecipitation and proximity ligation assay confirmed that both proteins could interact. MVI and AKAP9 colocalized at Rab5 containing early endosomes. Similarly to MVI, the amount of AKAP9 decreased during myoblast differentiation. However, in MVI-depleted cells, both cAMP and PKA levels were increased and a change in the MVI motor-dependent AKAP9 distribution was observed. Moreover, we found that PKA phosphorylated MVI-GT domain, thus implying functional relevance of MVI-AKAP9 interaction. We postulate that this novel interaction linking MVI with the PKA pathway could be important for targeting AKAP9-PKA complex within cells and/or providing PKA to phosphorylate MVI tail domain.

  16. Expanding the druggable space of the LSD1/CoREST epigenetic target: new potential binding regions for drug-like molecules, peptides, protein partners, and chromatin.

    Directory of Open Access Journals (Sweden)

    James C Robertson

    Full Text Available Lysine specific demethylase-1 (LSD1/KDM1A in complex with its corepressor protein CoREST is a promising target for epigenetic drugs. No therapeutic that targets LSD1/CoREST, however, has been reported to date. Recently, extended molecular dynamics (MD simulations indicated that LSD1/CoREST nanoscale clamp dynamics is regulated by substrate binding and highlighted key hinge points of this large-scale motion as well as the relevance of local residue dynamics. Prompted by the urgent need for new molecular probes and inhibitors to understand LSD1/CoREST interactions with small-molecules, peptides, protein partners, and chromatin, we undertake here a configurational ensemble approach to expand LSD1/CoREST druggability. The independent algorithms FTMap and SiteMap and our newly developed Druggable Site Visualizer (DSV software tool were used to predict and inspect favorable binding sites. We find that the hinge points revealed by MD simulations at the SANT2/Tower interface, at the SWIRM/AOD interface, and at the AOD/Tower interface are new targets for the discovery of molecular probes to block association of LSD1/CoREST with chromatin or protein partners. A fourth region was also predicted from simulated configurational ensembles and was experimentally validated to have strong binding propensity. The observation that this prediction would be prevented when using only the X-ray structures available (including the X-ray structure bound to the same peptide underscores the relevance of protein dynamics in protein interactions. A fifth region was highlighted corresponding to a small pocket on the AOD domain. This study sets the basis for future virtual screening campaigns targeting the five novel regions reported herein and for the design of LSD1/CoREST mutants to probe LSD1/CoREST binding with chromatin and various protein partners.

  17. Protein Interacting with C Kinase 1 (PICK1) Reduces Reinsertion Rates of Interaction Partners Sorted to Rab11-dependent Slow Recycling Pathway*

    Science.gov (United States)

    Madsen, Kenneth L.; Thorsen, Thor S.; Rahbek-Clemmensen, Troels; Eriksen, Jacob; Gether, Ulrik

    2012-01-01

    The scaffolding protein PICK1 (protein interacting with C kinase 1) contains an N-terminal PSD-95/Discs large/ZO-1 (PDZ) domain and a central lipid-binding Bin/amphiphysin/Rvs (BAR) domain. PICK1 is thought to regulate trafficking of its PDZ binding partners but different and even opposing functions have been suggested. Here, we apply ELISA-based assays and confocal microscopy in HEK293 cells with inducible PICK1 expression to assess in an isolated system the ability of PICK1 to regulate trafficking of natural and engineered PDZ binding partners. The dopamine transporter (DAT), which primarily sorts to degradation upon internalization, did not form perinuclear clusters with PICK1, and PICK1 did not affect DAT internalization/recycling. However, transfer of the PICK1-binding DAT C terminus to the β2-adrenergic receptor, which sorts to recycling upon internalization, led to formation of PICK1 co-clusters in Rab11-positive compartments. Furthermore, PICK1 inhibited Rab11-mediated recycling of the receptor in a BAR and PDZ domain-dependent manner. In contrast, transfer of the DAT C terminus to the δ-opioid receptor, which sorts to degradation, did not result in PICK1 co-clusters or any change in internalization/recycling. Further support for a role of PICK1 determined by its PDZ cargo was obtained for the PICK1 interaction partner prolactin-releasing peptide receptor (GPR10). GPR10 co-localized with Rab11 and clustered with PICK1 upon constitutive internalization but co-localized with the late endosomal marker Rab7 and did not cluster with PICK1 upon agonist-induced internalization. Our data suggest a selective role of PICK1 in clustering and reducing the recycling rates of PDZ domain binding partners sorted to the Rab11-dependent recycling pathway. PMID:22303009

  18. Identification of novel oxidized protein substrates and physiological partners of the mitochondrial ATP-dependent Lon-like protease Pim1

    DEFF Research Database (Denmark)

    Bayot, Aurélien; Gareil, Monique; Rogowska-Wrzesinska, Adelina

    2010-01-01

    ATP-dependent proteases are currently emerging as key regulators of mitochondrial functions. Among these proteolytic systems, Pim1, a Lon-like serine protease in Saccharomyces cerevisiae, is involved in the control of selective protein turnover in the mitochondrial matrix. In the absence of Pim1......, yeast cells have been shown to accumulate electron-dense inclusion bodies in the matrix space, to lose integrity of mitochondrial genome, and to be respiration-deficient. Because of the severity of phenotypes associated with the depletion of Pim1, this protease appears to be an essential component...... of oxidized protein substrates and physiological partners of Pim1 protease under non-repressing growth conditions. The results presented here supply evidence that Pim1-mediated proteolysis is required for elimination of oxidatively damaged proteins in mitochondria....

  19. Social Partners

    DEFF Research Database (Denmark)

    Tikkanen, Tarja; Hansen, Leif Emil; Guðmundsson, Bernharður

    2012-01-01

    based on a survey carried out in the Nordic countries in the regie of Nordic Council of Ministries the article deals with the role of social partners in senior and older workers policies and practises......based on a survey carried out in the Nordic countries in the regie of Nordic Council of Ministries the article deals with the role of social partners in senior and older workers policies and practises...

  20. A single amino acid change within the R2 domain of the VvMYB5b transcription factor modulates affinity for protein partners and target promoters selectivity

    Directory of Open Access Journals (Sweden)

    Granier Thierry

    2011-08-01

    Full Text Available Abstract Background Flavonoid pathway is spatially and temporally controlled during plant development and the transcriptional regulation of the structural genes is mostly orchestrated by a ternary protein complex that involves three classes of transcription factors (R2-R3-MYB, bHLH and WDR. In grapevine (Vitis vinifera L., several MYB transcription factors have been identified but the interactions with their putative bHLH partners to regulate specific branches of the flavonoid pathway are still poorly understood. Results In this work, we describe the effects of a single amino acid substitution (R69L located in the R2 domain of VvMYB5b and predicted to affect the formation of a salt bridge within the protein. The activity of the mutated protein (name VvMYB5bL, the native protein being referred as VvMYB5bR was assessed in different in vivo systems: yeast, grape cell suspensions, and tobacco. In the first two systems, VvMYB5bL exhibited a modified trans-activation capability. Moreover, using yeast two-hybrid assay, we demonstrated that modification of VvMYB5b transcriptional properties impaired its ability to correctly interact with VvMYC1, a grape bHLH protein. These results were further substantiated by overexpression of VvMYB5bR and VvMYB5bL genes in tobacco. Flowers from 35S::VvMYB5bL transgenic plants showed a distinct phenotype in comparison with 35S::VvMYB5bR and the control plants. Finally, significant differences in transcript abundance of flavonoid metabolism genes were observed along with variations in pigments accumulation. Conclusions Taken together, our findings indicate that VvMYB5bL is still able to bind DNA but the structural consequences linked to the mutation affect the capacity of the protein to activate the transcription of some flavonoid genes by modifying the interaction with its co-partner(s. In addition, this study underlines the importance of an internal salt bridge for protein conformation and thus for the establishment

  1. PARTNER Project

    CERN Multimedia

    Ballantine, A; Dixon-Altaber, H; Dosanjh, M; Kuchina, L

    2011-01-01

    Hadrontherapy uses particle beams to treat tumours located near critical organs and tumours that respond poorly to conventional radiation therapy. It has become evident that there is an emerging need for reinforcing research in hadrontherapy and it is essential to train professionals in this rapidly developing field. PARTNER is a 4-year Marie Curie Training project funded by the European Commission with 5.6 million Euros aimed at the creation of the next generation of experts. Ten academic institutes and research centres and two leading companies are participating in PARTNER, that is coordinated by CERN, forming a unique multidisciplinary and multinational European network. The project offers research and training opportunities to 25 young biologists, engineers, physicians and physicists and is allowing them to actively develop modern techniques for treating cancer in close collaboration with leading European Institutions. For this purpose PARTNER relies on cutting edge research and technology development, ef...

  2. Sterile α Motif Domain Containing 9 Is a Novel Cellular Interacting Partner to Low-Risk Type Human Papillomavirus E6 Proteins.

    Directory of Open Access Journals (Sweden)

    Jia Wang

    Full Text Available Low-risk type human papillomavirus (HPV 6 and 11 infection causes recurrent respiratory papillomatosis (RRP and genital warts. RRP is the most common benign tumor of the larynx in children with frequent relapses. Repeated surgeries are often needed to improve vocal function and prevent life-threatening respiratory obstruction. Currently, there are no effective treatments available to completely eliminate these diseases, largely due to limited knowledge regarding their viral molecular pathogenesis. HPV E6 proteins contribute to cell immortalization by interacting with a variety of cellular proteins, which have been well studied for the high-risk type HPVs related to cancer progression. However, the functions of low-risk HPV E6 proteins are largely unknown. In this study, we report GST-pulldown coupled mass spectrometry analysis with low-risk HPV E6 proteins that identified sterile alpha motif domain containing 9 (SAMD9 as a novel interacting partner. We then confirmed the interaction between HPV-E6 and SAMD9 using co-immunoprecipitation, proximity ligation assay, and confocal immunofluorescence staining. The SAMD9 gene is down-regulated in a variety of neoplasms and deleteriously mutated in normophosphatemic familial tumoral calcinosis. Interestingly, SAMD9 also has antiviral functions against poxvirus. Our study adds to the limited knowledge of the molecular properties of low-risk HPVs and describes new potential functions for the low-risk HPV E6 protein.

  3. A tissue-specific protein purification approach in Caenorhabditis elegans identifies novel interaction partners of DLG-1/Discs large.

    NARCIS (Netherlands)

    Waaijers, S.; Munoz, J.; Berends, C.; Ramalho, J.J.; Goerdayal, S.S.; Low, T.Y.; Zoumaro-Djayoon, A.D.; Hoffmann, M.; Koorman, T.; Tas, R.P.; Harterink, M.; Seelk, S.; Kerver, J.; Hoogenraad, C.C.; Bossinger, O.; Tursun, B.; Heuvel, S. van den; Heck, A.J.R. van; Boxem, M.

    2016-01-01

    BACKGROUND: Affinity purification followed by mass spectrometry (AP/MS) is a widely used approach to identify protein interactions and complexes. In multicellular organisms, the accurate identification of protein complexes by AP/MS is complicated by the potential heterogeneity of complexes in

  4. Single-step affinity purification of recombinant proteins using the silica-binding Si-tag as a fusion partner.

    Science.gov (United States)

    Ikeda, Takeshi; Ninomiya, Ken-ichi; Hirota, Ryuichi; Kuroda, Akio

    2010-05-01

    We previously reported that a silica-binding protein, designated Si-tag, can be used as a fusion tag to immobilize functional proteins on silica surfaces. In this study, by taking advantage of the strong affinity of Si-tag for silica, we developed a single-step purification method for Si-tagged fusion proteins. We utilized unmodified bare silica particles as a specific adsorbent and a high concentration of MgCl(2) solution as an elution buffer. A fusion protein of Si-tag and immunoglobulin-binding staphylococcal protein A, designated Si-tagged protein A, was recovered with a purity of 87+/-3% and yield of 84+/-4% from a crude extract of recombinant Escherichia coli. The simplicity of our method enables rapid, cost-effective purification of Si-tagged fusion proteins. We also discuss the mechanism of binding and dissociation of Si-tag and silica surfaces, and we suggest that the unusual basicity and disordered structure of the Si-tag polypeptide play important roles in the binding to silica. Copyright (c) 2009 Elsevier Inc. All rights reserved.

  5. Physical interaction between the strawberry allergen Fra a 1 and an associated partner FaAP: Interaction of Fra a 1 proteins and FaAP.

    Science.gov (United States)

    Franz-Oberdorf, Katrin; Langer, Andreas; Strasser, Ralf; Isono, Erika; Ranftl, Quirin L; Wunschel, Christian; Schwab, Wilfried

    2017-10-01

    The strawberry fruit allergens Fra a 1.01E, Fra a 1.02 and Fra a 1.03 belong to the group of pathogenesis-related 10 (PR-10) proteins and are homologs of the major birch pollen Bet v 1 and apple allergen Mal d 1. Bet v 1 related proteins are the most extensively studied allergens but their physiological function in planta remains elusive. Since Mal d 1-Associated Protein has been previously identified as interaction partner of Mal d 1 we studied the binding of the orthologous Fra a 1-Associated Protein (FaAP) to Fra a 1.01E/1.02/1.03. As the C-terminal sequence of FaAP showed strong auto-activation activity in yeast 2-hybrid analysis a novel time resolved DNA-switching system was successfully applied. Fra a 1.01E, Fra a 1.02, and Fra a 1.03 bind to FaAP with K D of 4.5 ± 1.1, 15 ± 3, and 11 ± 2 nM, respectively. Fra a 1.01E forms a dimer, whereas Fra a 1.02 and Fra a 1.03 bind as monomer. The results imply that PR-10 proteins might be integrated into a protein-interaction network and FaAP binding appears to be essential for the physiological function of the Fra a 1 proteins. © 2017 Wiley Periodicals, Inc.

  6. Stromal response to prostate cancer: nanotechnology-based detection of thioredoxin-interacting protein partners distinguishes prostate cancer associated stroma from that of benign prostatic hyperplasia.

    Science.gov (United States)

    Singer, Elizabeth; Linehan, Jennifer; Babilonia, Gail; Imam, S Ashraf; Smith, David; Loera, Sofia; Wilson, Timothy; Smith, Steven

    2013-01-01

    Histological staining of reactive stroma has been shown to be a predictor of biochemical recurrence in prostate cancer, however, molecular markers of the stromal response to prostate cancer have not yet been fully delineated. The objective of this study was to determine whether or not the stromal biomarkers detected with a thioredoxin-targeted nanodevice could be used to distinguish the stroma associated with benign prostatic hyperplasia from that associated with PCA. In this regard, we recently demonstrated that a thioredoxin-targeted nanodevice selectively binds to reactive stroma in frozen prostate tumor tissue sections. To accomplish this, random frozen prostate tissue sections from each of 35 patients who underwent resection were incubated with the nanodevice and graded for fluorescent intensity. An adjacent section from each case was stained with Hematoxylin & Eosin to confirm the diagnosis. Select cases were stained with Masson's Trichrome or immunohistochemically using antibodies to thioredoxin reductase 1, thioredoxin reductase 2 or peroxiredoxin 1. Our results demonstrate that the graded intensity of nanodevice binding to the stroma associated with PCA was significantly higher (p = 0.0127) than that of benign prostatic hyperplasia using the t-test. Immunohistochemical staining of adjacent sections in representative cases showed that none of the two commonly studied thioredoxin interacting protein partners mirrored the fluorescence pattern seen with the nanodevice. However, thioredoxin reductase 2 protein was clearly shown to be a biomarker of prostate cancer-associated reactive stroma whose presence distinguishes the stroma associated with benign prostatic hyperplasia from that associated with prostate cancer. We conclude that the signal detected by the nanodevice, in contrast to individual targets detected with antibodies used in this study, originates from multiple thioredoxin interacting protein partners that distinguish the M2 neutrophil and

  7. Stromal response to prostate cancer: nanotechnology-based detection of thioredoxin-interacting protein partners distinguishes prostate cancer associated stroma from that of benign prostatic hyperplasia.

    Directory of Open Access Journals (Sweden)

    Elizabeth Singer

    Full Text Available Histological staining of reactive stroma has been shown to be a predictor of biochemical recurrence in prostate cancer, however, molecular markers of the stromal response to prostate cancer have not yet been fully delineated. The objective of this study was to determine whether or not the stromal biomarkers detected with a thioredoxin-targeted nanodevice could be used to distinguish the stroma associated with benign prostatic hyperplasia from that associated with PCA. In this regard, we recently demonstrated that a thioredoxin-targeted nanodevice selectively binds to reactive stroma in frozen prostate tumor tissue sections. To accomplish this, random frozen prostate tissue sections from each of 35 patients who underwent resection were incubated with the nanodevice and graded for fluorescent intensity. An adjacent section from each case was stained with Hematoxylin & Eosin to confirm the diagnosis. Select cases were stained with Masson's Trichrome or immunohistochemically using antibodies to thioredoxin reductase 1, thioredoxin reductase 2 or peroxiredoxin 1. Our results demonstrate that the graded intensity of nanodevice binding to the stroma associated with PCA was significantly higher (p = 0.0127 than that of benign prostatic hyperplasia using the t-test. Immunohistochemical staining of adjacent sections in representative cases showed that none of the two commonly studied thioredoxin interacting protein partners mirrored the fluorescence pattern seen with the nanodevice. However, thioredoxin reductase 2 protein was clearly shown to be a biomarker of prostate cancer-associated reactive stroma whose presence distinguishes the stroma associated with benign prostatic hyperplasia from that associated with prostate cancer. We conclude that the signal detected by the nanodevice, in contrast to individual targets detected with antibodies used in this study, originates from multiple thioredoxin interacting protein partners that distinguish the M2

  8. Anti-HPV16 E2 protein T-cell responses and viral control in women with usual vulvar intraepithelial neoplasia and their healthy partners.

    Directory of Open Access Journals (Sweden)

    Simon Jacobelli

    Full Text Available T-cell responses (proliferation, intracellular cytokine synthesis and IFNγ ELISPOT against human papillomavirus 16 (HPV16 E2 peptides were tested during 18 months in a longitudinal study in eight women presenting with HPV16-related usual vulvar intraepithelial neoplasia (VIN and their healthy male partners. In six women, anti-E2 proliferative responses and cytokine production (single IFNγ and/or dual IFNγ/IL2 and/or single IL2 by CD4+ T lymphocytes became detectable after treating and healing of the usual VIN. In the women presenting with persistent lesions despite therapy, no proliferation was observed. Anti-E2 proliferative responses were also observed with dual IFNγ/IL2 production by CD4+ T-cells in six male partners who did not exhibit any genital HPV-related diseases. Ex vivo IFNγ ELISPOT showed numerous effector T-cells producing IFNγ after stimulation by a dominant E2 peptide in all men and women. Since the E2 protein is absent from the viral particles but is required for viral DNA replication, these results suggest a recent infection with replicative HPV16 in male partners. The presence of polyfunctional anti-E2 T-cell responses in the blood of asymptomatic men unambiguously establishes HPV infection even without detectable lesions. These results, despite the small size of the studied group, provide an argument in favor of prophylactic HPV vaccination of young men in order to prevent HPV16 infection and viral transmission from men to women.

  9. Anti-HPV16 E2 protein T-cell responses and viral control in women with usual vulvar intraepithelial neoplasia and their healthy partners.

    Science.gov (United States)

    Jacobelli, Simon; Sanaa, Fedoua; Moyal-Barracco, Micheline; Pelisse, Monique; Berville, Sophie; Villefroy, Pascale; North, Marie Odile; Figueiredo, Suzanne; Charmeteau, Bénédicte; Clerici, Thierry; Plantier, Françoise; Arnold, Françoise; Touzé, Antoine; Dupin, Nicolas; Avril, Marie-Françoise; Guillet, Jean-Gérard; Cheynier, Rémi; Bourgault-Villada, Isabelle

    2012-01-01

    T-cell responses (proliferation, intracellular cytokine synthesis and IFNγ ELISPOT) against human papillomavirus 16 (HPV16) E2 peptides were tested during 18 months in a longitudinal study in eight women presenting with HPV16-related usual vulvar intraepithelial neoplasia (VIN) and their healthy male partners. In six women, anti-E2 proliferative responses and cytokine production (single IFNγ and/or dual IFNγ/IL2 and/or single IL2) by CD4+ T lymphocytes became detectable after treating and healing of the usual VIN. In the women presenting with persistent lesions despite therapy, no proliferation was observed. Anti-E2 proliferative responses were also observed with dual IFNγ/IL2 production by CD4+ T-cells in six male partners who did not exhibit any genital HPV-related diseases. Ex vivo IFNγ ELISPOT showed numerous effector T-cells producing IFNγ after stimulation by a dominant E2 peptide in all men and women. Since the E2 protein is absent from the viral particles but is required for viral DNA replication, these results suggest a recent infection with replicative HPV16 in male partners. The presence of polyfunctional anti-E2 T-cell responses in the blood of asymptomatic men unambiguously establishes HPV infection even without detectable lesions. These results, despite the small size of the studied group, provide an argument in favor of prophylactic HPV vaccination of young men in order to prevent HPV16 infection and viral transmission from men to women.

  10. Social Partners

    DEFF Research Database (Denmark)

    Hansen, Leif Emil

    2011-01-01

    The purpose of the paper is to present findings from a new Nordic survey on social partners’ policy and practice in regards older workers. The goal of the survey was to find out to what extent the social partners have developed policies and outlined strategies, which explicitly address...... the demographic change and promote opportunities for lifelong learning and career development among their senior members (45+). Workforce in the Nordic countries tend to be highly organised – especially the older workers. The social partners’ involvement in the discussion of sustainable society....... The findings showed, on one hand, that while some social partners have started very good work, for many the issues of lifelong learning and opportunities for career development for older workers are not on their agenda. Besides differences between the unions in regards many aspects and within most countries...

  11. How does Tremblaya princeps get essential proteins from its nested partner Moranella endobia in the Mealybug Planoccocus citri?

    Science.gov (United States)

    López-Madrigal, Sergio; Balmand, Séverine; Latorre, Amparo; Heddi, Abdelaziz; Moya, Andrés; Gil, Rosario

    2013-01-01

    Many insects maintain intracellular mutualistic symbiosis with a wide range of bacteria which are considered essential for their survival (primary or P-endosymbiont) and typically suffer drastic genome degradation. Progressive loss of P-endosymbiont metabolic capabilities could lead to the recruitment of co-existent facultative endosymbiont (secondary or S-endosymbiont), thus adding more complexity to the symbiotic system. Planococcus citri, among other mealybug species, harbors an unconventional nested endosymbiotic system where every Tremblaya princeps cell (β-proteobacterium) harbors many Moranella endobia cells (γ-proteobacterium). In this system, T. princeps possess one of the smallest prokaryote genome known so far. This extreme genome reduction suggests the supply of many metabolites and essential gene products by M. endobia. Although sporadic cell lysis is plausible, the bacterial participation on the regulation of the predicted molecular exchange (at least to some extent) cannot be excluded. Although the comprehensive analysis of the protein translocation ability of M. endobia PCVAL rules out the existence of specific mechanisms for the exportation of proteins from M. endobia to T. princeps, immunolocation of two M. endobia proteins points towards a non-massive but controlled protein provision. We propose a sporadic pattern for the predicted protein exportation events, which could be putatively controlled by the host and/or mediated by local osmotic stress.

  12. How does Tremblaya princeps get essential proteins from its nested partner Moranella endobia in the Mealybug Planoccocus citri?

    Directory of Open Access Journals (Sweden)

    Sergio López-Madrigal

    Full Text Available Many insects maintain intracellular mutualistic symbiosis with a wide range of bacteria which are considered essential for their survival (primary or P-endosymbiont and typically suffer drastic genome degradation. Progressive loss of P-endosymbiont metabolic capabilities could lead to the recruitment of co-existent facultative endosymbiont (secondary or S-endosymbiont, thus adding more complexity to the symbiotic system. Planococcus citri, among other mealybug species, harbors an unconventional nested endosymbiotic system where every Tremblaya princeps cell (β-proteobacterium harbors many Moranella endobia cells (γ-proteobacterium. In this system, T. princeps possess one of the smallest prokaryote genome known so far. This extreme genome reduction suggests the supply of many metabolites and essential gene products by M. endobia. Although sporadic cell lysis is plausible, the bacterial participation on the regulation of the predicted molecular exchange (at least to some extent cannot be excluded. Although the comprehensive analysis of the protein translocation ability of M. endobia PCVAL rules out the existence of specific mechanisms for the exportation of proteins from M. endobia to T. princeps, immunolocation of two M. endobia proteins points towards a non-massive but controlled protein provision. We propose a sporadic pattern for the predicted protein exportation events, which could be putatively controlled by the host and/or mediated by local osmotic stress.

  13. Olefin cross-metathesis on proteins: investigation of allylic chalcogen effects and guiding principles in metathesis partner selection.

    Science.gov (United States)

    Lin, Yuya A; Chalker, Justin M; Davis, Benjamin G

    2010-12-01

    Olefin metathesis has recently emerged as a viable reaction for chemical protein modification. The scope and limitations of olefin metathesis in bioconjugation, however, remain unclear. Herein we report an assessment of various factors that contribute to productive cross-metathesis on protein substrates. Sterics, substrate scope, and linker selection are all considered. It was discovered during this investigation that allyl chalcogenides generally enhance the rate of alkene metathesis reactions. Allyl selenides were found to be exceptionally reactive olefin metathesis substrates, enabling a broad range of protein modifications not previously possible. The principles considered in this report are important not only for expanding the repertoire of bioconjugation but also for the application of olefin metathesis in general synthetic endeavors.

  14. The flexible C-terminal arm of the Lassa arenavirus Z-protein mediates interactions with multiple binding partners.

    Science.gov (United States)

    May, Eric R; Armen, Roger S; Mannan, Aristotle M; Brooks, Charles L

    2010-08-01

    The arenavirus genome encodes for a Z-protein, which contains a RING domain that coordinates two zinc ions, and has been identified as having several functional roles at various stages of the virus life cycle. Z-protein binds to multiple host proteins and has been directly implicated in the promotion of viral budding, repression of mRNA translation, and apoptosis of infected cells. Using homology models of the Z-protein from Lassa strain arenavirus, replica exchange molecular dynamics (MD) was used to refine the structures, which were then subsequently clustered. Population-weighted ensembles of low-energy cluster representatives were predicted based upon optimal agreement of the chemical shifts computed with the SPARTA program with the experimental NMR chemical shifts. A member of the refined ensemble was identified to be a potential binder of budding factor Tsg101 based on its correspondence to the structure of the HIV-1 Gag late domain when bound to Tsg101. Members of these ensembles were docked against the crystal structure of human eIF4E translation initiation factor. Two plausible binding modes emerged based upon their agreement with experimental observation, favorable interaction energies and stability during MD trajectories. Mutations to Z are proposed that would either inhibit both binding mechanisms or selectively inhibit only one mode. The C-terminal domain conformation of the most populated member of the representative ensemble shielded protein-binding recognition motifs for Tsg101 and eIF4E and represents the most populated state free in solution. We propose that C-terminal flexibility is key for mediating the different functional states of the Z-protein. (c) 2010 Wiley-Liss, Inc.

  15. Homeodomain-interacting protein kinase 2, a novel autoimmune regulator interaction partner, modulates promiscuous gene expression in medullary thymic epithelial cells.

    Science.gov (United States)

    Rattay, Kristin; Claude, Janine; Rezavandy, Esmail; Matt, Sonja; Hofmann, Thomas G; Kyewski, Bruno; Derbinski, Jens

    2015-02-01

    Promiscuous expression of a plethora of tissue-restricted Ags (TRAs) by medullary thymic epithelial cells (mTECs) plays an essential role in T cell tolerance. Although the cellular mechanisms by which promiscuous gene expression (pGE) imposes T cell tolerance have been well characterized, the underlying molecular mechanisms remain poorly understood. The autoimmune regulator (AIRE) is to date the only validated molecule known to regulate pGE. AIRE is part of higher-order multiprotein complexes, which promote transcription, elongation, and splicing of a wide range of target genes. How AIRE and its partners mediate these various effects at the molecular level is still largely unclear. Using a yeast two-hybrid screen, we searched for novel AIRE-interacting proteins and identified the homeodomain-interacting protein kinase 2 (HIPK2) as a novel partner. HIPK2 partially colocalized with AIRE in nuclear bodies upon cotransfection and in human mTECs in situ. Moreover, HIPK2 phosphorylated AIRE in vitro and suppressed the coactivator activity of AIRE in a kinase-dependent manner. To evaluate the role of Hipk2 in modulating the function of AIRE in vivo, we compared whole-genome gene signatures of purified mTEC subsets from TEC-specific Hipk2 knockout mice with control mice and identified a small set of differentially expressed genes. Unexpectedly, most differentially expressed genes were confined to the CD80(lo) mTEC subset and preferentially included AIRE-independent TRAs. Thus, although it modulates gene expression in mTECs and in addition affects the size of the medullary compartment, TEC-specific HIPK2 deletion only mildly affects AIRE-directed pGE in vivo. Copyright © 2015 by The American Association of Immunologists, Inc.

  16. Conserved Ankyrin Repeat Proteins and Their NIMA Kinase Partners Regulate Extracellular Matrix Remodeling and Intracellular Trafficking in Caenorhabditis elegans.

    Science.gov (United States)

    Lažetić, Vladimir; Fay, David S

    2017-01-01

    Molting is an essential developmental process in nematodes during which the epidermal apical extracellular matrix, the cuticle, is remodeled to accommodate further growth. Using genetic approaches, we identified a requirement for three conserved ankyrin repeat-rich proteins, MLT-2/ANKS6, MLT-3/ANKS3, and MLT-4/INVS, in Caenorhabditis elegans molting. Loss of mlt function resulted in severe defects in the ability of larvae to shed old cuticle and led to developmental arrest. Genetic analyses demonstrated that MLT proteins functionally cooperate with the conserved NIMA kinase family members NEKL-2/NEK8 and NEKL-3/NEK6/NEK7 to promote cuticle shedding. MLT and NEKL proteins were specifically required within the hyp7 epidermal syncytium, and fluorescently tagged mlt and nekl alleles were expressed in puncta within this tissue. Expression studies further showed that NEKL-2-MLT-2-MLT-4 and NEKL-3-MLT-3 colocalize within largely distinct assemblies of apical foci. MLT-2 and MLT-4 were required for the normal accumulation of NEKL-2 at the hyp7-seam cell boundary, and loss of mlt-2 caused abnormal nuclear accumulation of NEKL-2 Correspondingly, MLT-3, which bound directly to NEKL-3, prevented NEKL-3 nuclear localization, supporting the model that MLT proteins may serve as molecular scaffolds for NEKL kinases. Our studies additionally showed that the NEKL-MLT network regulates early steps in clathrin-mediated endocytosis at the apical surface of hyp7, which may in part account for molting defects observed in nekl and mlt mutants. This study has thus identified a conserved NEKL-MLT protein network that regulates remodeling of the apical extracellular matrix and intracellular trafficking, functions that may be conserved across species. Copyright © 2017 by the Genetics Society of America.

  17. The Sec1/Munc18 protein Vps45 regulates cellular levels of its SNARE binding partners Tlg2 and Snc2 in Saccharomyces cerevisiae.

    Directory of Open Access Journals (Sweden)

    Scott G Shanks

    Full Text Available Intracellular membrane trafficking pathways must be tightly regulated to ensure proper functioning of all eukaryotic cells. Central to membrane trafficking is the formation of specific SNARE (soluble N-ethylmeleimide-sensitive factor attachment protein receptor complexes between proteins on opposing lipid bilayers. The Sec1/Munc18 (SM family of proteins play an essential role in SNARE-mediated membrane fusion, and like the SNAREs are conserved through evolution from yeast to humans. The SM protein Vps45 is required for the formation of yeast endosomal SNARE complexes and is thus essential for traffic through the endosomal system. Here we report that, in addition to its role in regulating SNARE complex assembly, Vps45 regulates cellular levels of its SNARE binding partners: the syntaxin Tlg2 and the v-SNARE Snc2: Cells lacking Vps45 have reduced cellular levels of Tlg2 and Snc2; and elevation of Vps45 levels results in concomitant increases in the levels of both Tlg2 and Snc2. As well as regulating traffic through the endosomal system, the Snc v-SNAREs are also required for exocytosis. Unlike most vps mutants, cells lacking Vps45 display multiple growth phenotypes. Here we report that these can be reversed by selectively restoring Snc2 levels in vps45 mutant cells. Our data indicate that as well as functioning as part of the machinery that controls SNARE complex assembly, Vps45 also plays a key role in determining the levels of its cognate SNARE proteins; another key factor in regulation of membrane traffic.

  18. Proteomic analysis of HIV-1 Nef cellular binding partners reveals a role for exocyst complex proteins in mediating enhancement of intercellular nanotube formation

    Directory of Open Access Journals (Sweden)

    Mukerji Joya

    2012-06-01

    Full Text Available Abstract Background HIV-1 Nef protein contributes to pathogenesis via multiple functions that include enhancement of viral replication and infectivity, alteration of intracellular trafficking, and modulation of cellular signaling pathways. Nef stimulates formation of tunneling nanotubes and virological synapses, and is transferred to bystander cells via these intercellular contacts and secreted microvesicles. Nef associates with and activates Pak2, a kinase that regulates T-cell signaling and actin cytoskeleton dynamics, but how Nef promotes nanotube formation is unknown. Results To identify Nef binding partners involved in Pak2-association dependent Nef functions, we employed tandem mass spectrometry analysis of Nef immunocomplexes from Jurkat cells expressing wild-type Nef or Nef mutants defective for the ability to associate with Pak2 (F85L, F89H, H191F and A72P, A75P in NL4-3. We report that wild-type, but not mutant Nef, was associated with 5 components of the exocyst complex (EXOC1, EXOC2, EXOC3, EXOC4, and EXOC6, an octameric complex that tethers vesicles at the plasma membrane, regulates polarized exocytosis, and recruits membranes and proteins required for nanotube formation. Additionally, Pak2 kinase was associated exclusively with wild-type Nef. Association of EXOC1, EXOC2, EXOC3, and EXOC4 with wild-type, but not mutant Nef, was verified by co-immunoprecipitation assays in Jurkat cells. Furthermore, shRNA-mediated depletion of EXOC2 in Jurkat cells abrogated Nef-mediated enhancement of nanotube formation. Using bioinformatic tools, we visualized protein interaction networks that reveal functional linkages between Nef, the exocyst complex, and the cellular endocytic and exocytic trafficking machinery. Conclusions Exocyst complex proteins are likely a key effector of Nef-mediated enhancement of nanotube formation, and possibly microvesicle secretion. Linkages revealed between Nef and the exocyst complex suggest a new paradigm of

  19. Sarco(endo)plasmic reticulum ATPase is a molecular partner of Wolfram syndrome 1 protein, which negatively regulates its expression

    Science.gov (United States)

    Zatyka, Malgorzata; Da Silva Xavier, Gabriela; Bellomo, Elisa A.; Leadbeater, Wendy; Astuti, Dewi; Smith, Joel; Michelangeli, Frank; Rutter, Guy A.; Barrett, Timothy G.

    2015-01-01

    Wolfram syndrome is an autosomal recessive disorder characterized by neurodegeneration and diabetes mellitus. The gene responsible for the syndrome (WFS1) encodes an endoplasmic reticulum (ER)-resident transmembrane protein that is involved in the regulation of the unfolded protein response (UPR), intracellular ion homeostasis, cyclic adenosine monophosphate production and regulation of insulin biosynthesis and secretion. In this study, single cell Ca2+ imaging with fura-2 and direct measurements of free cytosolic ATP concentration ([ATP]CYT) with adenovirally expressed luciferase confirmed a reduced and delayed rise in cytosolic free Ca2+ concentration ([Ca2+]CYT), and additionally, diminished [ATP]CYT rises in response to elevated glucose concentrations in WFS1-depleted MIN6 cells. We also observed that sarco(endo)plasmic reticulum ATPase (SERCA) expression was elevated in several WFS1-depleted cell models and primary islets. We demonstrated a novel interaction between WFS1 and SERCA by co-immunoprecipitation in Cos7 cells and with endogenous proteins in human neuroblastoma cells. This interaction was reduced when cells were treated with the ER stress inducer dithiothreitol. Treatment of WFS1-depleted neuroblastoma cells with the proteasome inhibitor MG132 resulted in reduced accumulation of SERCA levels compared with wild-type cells. Together these results reveal a role for WFS1 in the negative regulation of SERCA and provide further insights into the function of WFS1 in calcium homeostasis. PMID:25274773

  20. Collagen XII and XIV, New Partners of Cartilage Oligomeric Matrix Protein in the Skin Extracellular Matrix Suprastructure*

    Science.gov (United States)

    Agarwal, Pallavi; Zwolanek, Daniela; Keene, Douglas R.; Schulz, Jan-Niklas; Blumbach, Katrin; Heinegård, Dick; Zaucke, Frank; Paulsson, Mats; Krieg, Thomas; Koch, Manuel; Eckes, Beate

    2012-01-01

    The tensile and scaffolding properties of skin rely on the complex extracellular matrix (ECM) that surrounds cells, vasculature, nerves, and adnexus structures and supports the epidermis. In the skin, collagen I fibrils are the major structural component of the dermal ECM, decorated by proteoglycans and by fibril-associated collagens with interrupted triple helices such as collagens XII and XIV. Here we show that the cartilage oligomeric matrix protein (COMP), an abundant component of cartilage ECM, is expressed in healthy human skin. COMP expression is detected in the dermal compartment of skin and in cultured fibroblasts, whereas epidermis and HaCaT cells are negative. In addition to binding collagen I, COMP binds to collagens XII and XIV via their C-terminal collagenous domains. All three proteins codistribute in a characteristic narrow zone in the superficial papillary dermis of healthy human skin. Ultrastructural analysis by immunogold labeling confirmed colocalization and further revealed the presence of COMP along with collagens XII and XIV in anchoring plaques. On the basis of these observations, we postulate that COMP functions as an adapter protein in human skin, similar to its function in cartilage ECM, by organizing collagen I fibrils into a suprastructure, mainly in the vicinity of anchoring plaques that stabilize the cohesion between the upper dermis and the basement membrane zone. PMID:22573329

  1. Bioinformatic evidence for a widely distributed, ribosomally produced electron carrier precursor, its maturation proteins, and its nicotinoprotein redox partners

    Directory of Open Access Journals (Sweden)

    Haft Daniel H

    2011-01-01

    Full Text Available Abstract Background Enzymes in the radical SAM (rSAM domain family serve in a wide variety of biological processes, including RNA modification, enzyme activation, bacteriocin core peptide maturation, and cofactor biosynthesis. Evolutionary pressures and relationships to other cellular constituents impose recognizable grammars on each class of rSAM-containing system, shaping patterns in results obtained through various comparative genomics analyses. Results An uncharacterized gene cluster found in many Actinobacteria and sporadically in Firmicutes, Chloroflexi, Deltaproteobacteria, and one Archaeal plasmid contains a PqqE-like rSAM protein family that includes Rv0693 from Mycobacterium tuberculosis. Members occur clustered with a strikingly well-conserved small polypeptide we designate "mycofactocin," similar in size to bacteriocins and PqqA, precursor of pyrroloquinoline quinone (PQQ. Partial Phylogenetic Profiling (PPP based on the distribution of these markers identifies the mycofactocin cluster, but also a second tier of high-scoring proteins. This tier, strikingly, is filled with up to thirty-one members per genome from three variant subfamilies that occur, one each, in three unrelated classes of nicotinoproteins. The pattern suggests these variant enzymes require not only NAD(P, but also the novel gene cluster. Further study was conducted using SIMBAL, a PPP-like tool, to search these nicotinoproteins for subsequences best correlated across multiple genomes to the presence of mycofactocin. For both the short chain dehydrogenase/reductase (SDR and iron-containing dehydrogenase families, aligning SIMBAL's top-scoring sequences to homologous solved crystal structures shows signals centered over NAD(P-binding sites rather than over substrate-binding or active site residues. Previous studies on some of these proteins have revealed a non-exchangeable NAD cofactor, such that enzymatic activity in vitro requires an artificial electron acceptor such

  2. Independent evolution of tetraloop in enterovirus oriL replicative element and its putative binding partners in virus protein 3C

    Science.gov (United States)

    Deviatkin, Andrei A.; Tcelykh, Irina O.; Lukashev, Alexander N.; Gmyl, Anatoly P.

    2017-01-01

    Background Enteroviruses are small non-enveloped viruses with a (+) ssRNA genome with one open reading frame. Enterovirus protein 3C (or 3CD for some species) binds the replicative element oriL to initiate replication. The replication of enteroviruses features a low-fidelity process, which allows the virus to adapt to the changing environment on the one hand, and requires additional mechanisms to maintain the genome stability on the other. Structural disturbances in the apical region of oriL domain d can be compensated by amino acid substitutions in positions 154 or 156 of 3C (amino acid numeration corresponds to poliovirus 3C), thus suggesting the co-evolution of these interacting sequences in nature. The aim of this work was to understand co-evolution patterns of two interacting replication machinery elements in enteroviruses, the apical region of oriL domain d and its putative binding partners in the 3C protein. Methods To evaluate the variability of the domain d loop sequence we retrieved all available full enterovirus sequences (>6, 400 nucleotides), which were present in the NCBI database on February 2017 and analysed the variety and abundance of sequences in domain d of the replicative element oriL and in the protein 3C. Results A total of 2,842 full genome sequences was analysed. The majority of domain d apical loops were tetraloops, which belonged to consensus YNHG (Y = U/C, N = any nucleotide, H = A/C/U). The putative RNA-binding tripeptide 154–156 (Enterovirus C 3C protein numeration) was less diverse than the apical domain d loop region and, in contrast to it, was species-specific. Discussion Despite the suggestion that the RNA-binding tripeptide interacts with the apical region of domain d, they evolve independently in nature. Together, our data indicate the plastic evolution of both interplayers of 3C-oriL recognition. PMID:29018627

  3. Identification of the ankyrin repeat proteins ANKRA and RFXANK as novel partners of class IIa histone deacetylases.

    Science.gov (United States)

    Wang, Audrey H; Grégoire, Serge; Zika, Eleni; Xiao, Lin; Li, Cathy S; Li, Hongwei; Wright, Kenneth L; Ting, Jenny P; Yang, Xiang-Jiao

    2005-08-12

    Eighteen human histone deacetylases (HDACs) have been identified, and according to their sequence similarity to yeast homologs, these enzymes are grouped into distinct classes. Within class II, HDAC4, HDAC5, HDAC7, and HDAC9 share similar domain organization both within the N-terminal extension and the C-terminal catalytic domain, thus forming a subclass known as class IIa. These HDACs function as signal-responsive transcriptional corepressors. To gain further insight into their function and regulation, we utilized an N-terminal fragment of HDAC4 as bait in yeast two-hybrid screens, which uncovered myocyte enhancer factor 2C, 14-3-3zeta, and ankyrin repeat family A protein (ANKRA). ANKRA is a poorly characterized protein with an ankyrin repeat domain similar to RFXANK, a subunit of the trimeric transcription factor RFX. Mutations on genes of the RFX subunits and the coactivator CIITA are responsible for the bare lymphocyte syndrome, an immunodeficiency disorder attributed to the lack of major histocompatibility complex class II (MHCII) antigens. Through its ankyrin repeat domain, RFXANK interacted with HDAC4. Two RFXANK-binding sites were found on HDAC4 with one located within residues 118-279 and another within residues 448-666. Interestingly, this deacetylase also interacted with CIITA. Consistent with the physical interaction with RFXANK and CIITA, HDAC4 and homologs repressed MHCII expression. These results identify ANKRA, RFXANK, and CIITA as novel targets of class IIa HDACs and suggest that these deacetylases play a role in regulating MHCII expression.

  4. Pepper protein phosphatase type 2C, CaADIP1 and its interacting partner CaRLP1 antagonistically regulate ABA signalling and drought response.

    Science.gov (United States)

    Lim, Chae Woo; Lee, Sung Chul

    2016-07-01

    Abscisic acid (ABA) is a key phytohormone that regulates plant growth and developmental processes, including seed germination and stomatal closing. Here, we report the identification and functional characterization of a novel type 2C protein phosphatase, CaADIP1 (Capsicum annuum ABA and Drought-Induced Protein phosphatase 1). The expression of CaADIP1 was induced in pepper leaves by ABA, drought and NaCl treatments. Arabidopsis plants overexpressing CaADIP1 (CaADIP1-OX) exhibited an ABA-hyposensitive and drought-susceptible phenotype. We used a yeast two-hybrid screening assay to identify CaRLP1 (Capsicum annuum RCAR-Like Protein 1), which interacts with CaADIP1 in the cytoplasm and nucleus. In contrast to CaADIP1-OX plants, CaRLP1-OX plants displayed an ABA-hypersensitive and drought-tolerant phenotype, which was characterized by low levels of transpirational water loss and increased expression of stress-responsive genes relative to those of wild-type plants. In CaADIP1-OX/CaRLP1-OX double transgenic plants, ectopic expression of the CaRLP1 gene led to strong suppression of CaADIP1-induced ABA hyposensitivity during the germinative and post-germinative stages, indicating that CaADIP1 and CaRLP1 act in the same signalling pathway and CaADIP1 functions downstream of CaRLP1. Our results indicate that CaADIP1 and its interacting partner CaRLP1 antagonistically regulate the ABA-dependent defense signalling response to drought stress. © 2016 John Wiley & Sons Ltd.

  5. The Constitution of Partnering

    DEFF Research Database (Denmark)

    Gottlieb, Stefan Christoffer

    The constitution of partnering. Afhandlingen behandler konstitueringen af ledelseskonceptet partnering og dets anvendelse i dansk byggeri. Partnering er et udbredt koncept i byggeriet som betoner samarbejde, tillid og gensidighed mellem de deltagende parter, og konceptet har de senere år har været...... om, hvordan partnering som koncept er fremkommet og har udviklet sig og hvilke konstitutive effekter partnering har på byggeriets praksis. Det teoretiske udgangspunkt i Foucault begrundes i behovet for at fastholde en mangefacetteret og historisk analyse af partnering i modsætning til de...... ledelseskoncept (partnering) medvirker til at strukturere byggeprocesser ved at skabe bestemte muligheder for handling samtidig med at der opretholdes en betydelig stabilitet. Afhandlingen har en række praktiske implikationer ved at identificere de dispositiver eller sociale teknologier som en forandring af...

  6. Phosphorylation of CtBP1 by cAMP-dependent Protein Kinase Modulates Induction of CYP17 by Stimulating Partnering of CtBP1 and 2*s

    Science.gov (United States)

    Dammer, Eric B.; Sewer, Marion B.

    2009-01-01

    In the human adrenal cortex, the peptide hormone adrenocorticotropin (ACTH) directs cortisol and adrenal androgen biosynthesis by activating a cAMP/cAMP-dependent protein kinase (PKA) pathway. Carboxyl-terminal binding protein 1 (CtBP1) is a corepressor that regulates transcription of the CYP17 gene by periodically interacting with steroidogenic factor-1 in response to ACTH signaling. Given that CtBP1 function is regulated by NADH binding, we hypothesized that ACTH-stimulated changes in cellular pyridine nucleotide concentrations modulate the ability of CtBP1 to repress CYP17 transcription. Further, we postulated that PKA evokes changes in the phosphorylation status of CtBP1 that control the ability of the protein to bind to steroidogenic factor-1 and the coactivator GCN5 (general control nonderepressed 5) and repress CYP17 gene expression. We show that ACTH alters pyridine nucleotide redox state and identify amino acid residues in CtBP1 that are targeted by PKA and PAK6. Both ACTH/cAMP signaling and NADH/NAD+ ratio stimulate nuclear-cytoplasmic oscillation of both CtBP proteins. We provide evidence that PKA 1) induces metabolic changes in the adrenal cortex and 2) phosphorylates CtBP proteins, particularly CtBP1 at T144, resulting in CtBP protein partnering and ACTH-dependent CYP17 transcription. PMID:18184656

  7. Partnering and contracting

    DEFF Research Database (Denmark)

    Bohnstedt, Kristian Ditlev

    2014-01-01

    Purpose - Partnering is often, by economists, and construction managerial literature related to more incomplete contracts. This can be explained by seeing partnering as something that neutralizes opportunism. The aim is to uncover whether partnering neutralizes opportunism when there is an incomp......Purpose - Partnering is often, by economists, and construction managerial literature related to more incomplete contracts. This can be explained by seeing partnering as something that neutralizes opportunism. The aim is to uncover whether partnering neutralizes opportunism when...... there is an incomplete contract or reduces transaction costs for renegotiation of complete contracts when new information arises. Design - The study is a cross-sectional design comprising document analysis and interviews. Findings - Firstly that partnering does not necessarily entail more incomplete contracts, which...... contradicts the incomplete contracting theory. Secondly, in complete contract setting partnering can be motivated when seen as a willingness to renegotiate complete contracts i.e. partnering lowers transaction costs for renegotiation. Partnering can make it rational for one party to accept disadvantageous...

  8. The reaction of neuroglobin with potential redox protein partners cytochrome b5  and cytochrome c

    DEFF Research Database (Denmark)

    Fago, Angela; Mathews, A.J.; Moens, L.

    2006-01-01

    Previously identified, potentially neuroprotective reactions of neuroglobin require the existence of yet unknown redox partners. We show here that the reduction of ferric neuroglobin by cytochrome b5 is relatively slow (k=6×102M-1s-1 at pH 7.0) and thus is unlikely to be of physiological signific...... significance. In contrast, the reaction between ferrous neuroglobin and ferric cytochrome c is very rapid (k=2×107M-1s-1) with an apparent overall equilibrium constant of 1μM. Based on this data we propose that ferrous neuroglobin may well play a role in preventing apoptosis...

  9. IDRC PARTNERING CYCLE

    International Development Research Centre (IDRC) Digital Library (Canada)

    IDRC CRDI

    1. Exploration: Opportunities for collaboration are identified, potential partners are qualified, risks are assessed, and a decision to work with one or more partners is made. Some of the activities taking place during the exploration stage are: researching and gathering intelligence; examining options; and confirming matching.

  10. Stratum, a Homolog of the Human GEF Mss4, Partnered with Rab8, Controls the Basal Restriction of Basement Membrane Proteins in Epithelial Cells

    Directory of Open Access Journals (Sweden)

    Olivier Devergne

    2017-02-01

    Full Text Available The basement membrane (BM, a sheet of extracellular matrix lining the basal side of epithelia, is essential for epithelial cell function and integrity, yet the mechanisms that control the basal restriction of BM proteins are poorly understood. In epithelial cells, a specialized pathway is dedicated to restrict the deposition of BM proteins basally. Here, we report the identification of a factor in this pathway, a homolog of the mammalian guanine nucleotide exchange factor (GEF Mss4, which we have named Stratum. The loss of Stratum leads to the missecretion of BM proteins at the apical side of the cells, forming aberrant layers in close contact with the plasma membrane. We found that Rab8GTPase acts downstream of Stratum in this process. Altogether, our results uncover the importance of this GEF/Rab complex in specifically coordinating the basal restriction of BM proteins, a critical process for the establishment and maintenance of epithelial cell polarity.

  11. Quantitative proteomics identifies Gemin5, a scaffolding protein involved in ribonucleoprotein assembly, as a novel partner for eukaryotic initiation factor 4E

    DEFF Research Database (Denmark)

    Fierro-Monti, Ivo; Mohammed, Shabaz; Matthiesen, Rune

    2006-01-01

    Protein complexes are dynamic entities; identification and quantitation of their components is critical in elucidating functional roles under specific cellular conditions. We report the first quantitative proteomic analysis of the human cap-binding protein complex. Components and proteins...... levels of Gemin5:eIF4E complexes were found in phorbol ester treated HEK293 cells. Gemin5 and eIF4E co-localized to cytoplasmic P-bodies in human osteosarcoma U2OS cells. Interaction between eIF4E and Gemin5 and their co-localization to the P-bodies, may serve to recruit capped mRNAs to these RNP...... complexes, for functions related to RNP assembly, remodeling and/or transition from active translation to mRNA degradation. Our results demonstrate that our quantitative proteomic strategy can be applied to the identification and quantitation of protein complex components in human cells grown under...

  12. Single-step affinity and cost-effective purification of recombinant proteins using the Sepharose-binding lectin-tag from the mushroom Laetiporus sulphureus as fusion partner.

    Science.gov (United States)

    Li, Xiao-Jing; Liu, Jin-Ling; Gao, Dong-Sheng; Wan, Wen-Yan; Yang, Xia; Li, Yong-Tao; Chang, Hong-Tao; Chen, Lu; Wang, Chuan-Qing; Zhao, Jun

    2016-03-01

    Previous research showed that a lectin from the mushroom Laetiporus sulphureus, designed LSL, bound to Sepharose and could be eluted by lactose. In this study, by taking advantage of the strong affinity of LSL-tag for Sepharose, we developed a single-step purification method for LSL-tagged fusion proteins. We utilized unmodified Sepharose-4B as a specific adsorbent and 0.2 M lactose solution as an elution buffer. Fusion proteins of LSL-tag and porcine circovirus capsid protein, designated LSL-Cap was recovered with purity of 90 ± 4%, and yield of 87 ± 3% from crude extract of recombinant Escherichia coli. To enable the remove of LSL-tag, tobacco etch virus (TEV) protease recognition sequence was placed downstream of LSL-tag in the expression vector, and LSL-tagged TEV protease, designated LSL-TEV, was also expressed in E. coli., and was recovered with purity of 82 ± 5%, and yield of 85 ± 2% from crude extract of recombinant E. coli. After digestion of LSL-tagged recombinant proteins with LSL-TEV, the LSL tag and LSL-TEV can be easily removed by passing the digested products through the Sepharose column. It is of worthy noting that the Sepharose can be reused after washing with PBS. The LSL affinity purification method enables rapid and inexpensive purification of LSL-tagged fusion proteins and scale-up production of native proteins. Copyright © 2015 Elsevier Inc. All rights reserved.

  13. Solution structure, membrane interactions, and protein binding partners of the tetraspanin Sm-TSP-2, a vaccine antigen from the human blood fluke Schistosoma mansoni.

    Science.gov (United States)

    Jia, Xinying; Schulte, Leigh; Loukas, Alex; Pickering, Darren; Pearson, Mark; Mobli, Mehdi; Jones, Alun; Rosengren, Karl J; Daly, Norelle L; Gobert, Geoffrey N; Jones, Malcolm K; Craik, David J; Mulvenna, Jason

    2014-03-07

    The tetraspanins (TSPs) are a family of integral membrane proteins that are ubiquitously expressed at the surface of eukaryotic cells. TSPs mediate a range of processes at the surface of the plasma membrane by providing a scaffold for the assembly of protein complexes known as tetraspanin-enriched microdomains (TEMs). We report here the structure of the surface-exposed EC2 domain from Sm-TSP-2, a TSP from Schistosoma mansoni and one of the better prospects for the development of a vaccine against schistosomiasis. This is the first solution structure of this domain, and our investigations of its interactions with lipid micelles provide a general model for interactions between TSPs, membranes, and other proteins. Using chemical cross-linking, eight potential protein constituents of Sm-TSP-2-mediated TEMs were also identified. These include proteins important for membrane maintenance and repair, providing further evidence for the functional role of Sm-TSP-2- and Sm-TSP-2-mediated TEMs. The identification of calpain, Sm29, and fructose-bisphosphate aldolase, themselves potential vaccine antigens, suggests that the Sm-TSP-2-mediated TEMs could be disrupted via multiple targets. The identification of further Sm-TSP-2-mediated TEM proteins increases the available candidates for multiplex vaccines and/or novel drugs targeting TEMs in the schistosome tegument.

  14. Solution Structure, Membrane Interactions, and Protein Binding Partners of the Tetraspanin Sm-TSP-2, a Vaccine Antigen from the Human Blood Fluke Schistosoma mansoni*

    Science.gov (United States)

    Jia, Xinying; Schulte, Leigh; Loukas, Alex; Pickering, Darren; Pearson, Mark; Mobli, Mehdi; Jones, Alun; Rosengren, Karl J.; Daly, Norelle L.; Gobert, Geoffrey N.; Jones, Malcolm K.; Craik, David J.; Mulvenna, Jason

    2014-01-01

    The tetraspanins (TSPs) are a family of integral membrane proteins that are ubiquitously expressed at the surface of eukaryotic cells. TSPs mediate a range of processes at the surface of the plasma membrane by providing a scaffold for the assembly of protein complexes known as tetraspanin-enriched microdomains (TEMs). We report here the structure of the surface-exposed EC2 domain from Sm-TSP-2, a TSP from Schistosoma mansoni and one of the better prospects for the development of a vaccine against schistosomiasis. This is the first solution structure of this domain, and our investigations of its interactions with lipid micelles provide a general model for interactions between TSPs, membranes, and other proteins. Using chemical cross-linking, eight potential protein constituents of Sm-TSP-2-mediated TEMs were also identified. These include proteins important for membrane maintenance and repair, providing further evidence for the functional role of Sm-TSP-2- and Sm-TSP-2-mediated TEMs. The identification of calpain, Sm29, and fructose-bisphosphate aldolase, themselves potential vaccine antigens, suggests that the Sm-TSP-2-mediated TEMs could be disrupted via multiple targets. The identification of further Sm-TSP-2-mediated TEM proteins increases the available candidates for multiplex vaccines and/or novel drugs targeting TEMs in the schistosome tegument. PMID:24429291

  15. Identifying Coevolving Partners from Paralogous Gene Families

    Directory of Open Access Journals (Sweden)

    Chen-Hsiang Yeang

    2008-01-01

    Full Text Available Many methods have been developed to detect coevolution from aligned sequences. However, all the existing methods require a one-to-one mapping of candidate coevolving partners (nucleotides, amino acids a priori. When two families of sequences have distinct duplication and loss histories, finding the one-to-one mapping of coevolving partners can be computationally involved. We propose an algorithm to identify the coevolving partners from two families of sequences with distinct phylogenetic trees. The algorithm maps each gene tree to a reference species tree, and builds a joint state of sequence composition and assignments of coevolving partners for each species tree node. By applying dynamic programming on the joint states, the optimal assignments can be identified. Time complexity is quadratic to the size of the species tree, and space complexity is exponential to the maximum number of gene tree nodes mapped to the same species tree node. Analysis on both simulated data and Pfam protein domain sequences demonstrates that the paralog coevolution algorithm picks up the coevolving partners with 60%–88% accuracy. This algorithm extends phylogeny-based coevolutionary models and make them applicable to a wide range of problems such as predicting protein-protein, protein-DNA and DNA-RNA interactions of two distinct families of sequences.

  16. βArrestins in Cardiac G Protein-Coupled Receptor Signaling and Function: Partners in Crime or “Good Cop, Bad Cop”?

    Directory of Open Access Journals (Sweden)

    Anastasios Lymperopoulos

    2013-12-01

    Full Text Available βarrestin (βarr-1 and -2 (βarrs (or Arrestin-2 and -3, respectively are universal G protein-coupled receptor (GPCR adapter proteins expressed abundantly in extra-retinal tissues, including the myocardium. Both were discovered in the lab of the 2012 Nobel Prize in Chemistry co-laureate Robert Lefkowitz, initially as terminators of signaling from the β-adrenergic receptor (βAR, a process known as functional desensitization. They are now known to switch GPCR signaling from G protein-dependent to G protein-independent, which, in the case of βARs and angiotensin II type 1 receptor (AT1R, might be beneficial, e.g., anti-apoptotic, for the heart. However, the specific role(s of each βarr isoform in cardiac GPCR signaling and function (or dysfunction in disease, remain unknown. The current consensus is that, whereas both βarr isoforms can desensitize and internalize cardiac GPCRs, they play quite different (even opposing in certain instances roles in the G protein-independent signaling pathways they initiate in the cardiovascular system, including in the myocardium. The present review will discuss the current knowledge in the field of βarrs and their roles in GPCR signaling and function in the heart, focusing on the three most important, for cardiac physiology, GPCR types (β1AR, β2AR & AT1R, and will also highlight important questions that currently remain unanswered.

  17. Solution Structure of Pfu RPP21, a Component of the Archaeal RNase P Holoenzyme, and Interactions with its RPP29 Protein Partner

    OpenAIRE

    Amero, Carlos D.; Boomershine, William P; Xu, Yiren; Foster, Mark

    2008-01-01

    RNase P is the ubiquitous ribonucleoprotein metalloenzyme responsible for cleaving the 5′-leader sequence of precursor tRNAs during their maturation. While the RNA subunit is catalytically active on its own at high monovalent and divalent ion concentration, four proteins subunits are associated with archaeal RNase P activity in vivo: RPP21, RPP29, RPP30 and POP5. These proteins have been shown to function in pairs: RPP21-RPP29 and POP5-RPP30. We have determined the solution structure of RPP21...

  18. Collateral Intimate Partner Homicide

    Directory of Open Access Journals (Sweden)

    Emily Meyer

    2013-04-01

    Full Text Available Collateral intimate partner homicide (CIPH is an underinvestigated genre of intimate partner violence (IPV where an individual(s connected to the IPV victim is murdered. We conducted a content analysis of a statewide database of CIPH newspaper articles (1990-2007. Out of 111 collateral murder victims, there were 84 IPV female focal victims and 84 male perpetrators. The most frequently reported CIPH decedent was the focal victim’s new partner (30%; 45% of focal victims were themselves killed. News reports framed CIPH as the unexpected result of interpersonal conflict, despite evidence of a systematic pattern of coercion and violence that capitulated in murder.

  19. The multivalent PDZ domain-containing protein CIPP is a partner of acid-sensing ion channel 3 in sensory neurons.

    Science.gov (United States)

    Anzai, Naohiko; Deval, Emmanuel; Schaefer, Lionel; Friend, Valerie; Lazdunski, Michel; Lingueglia, Eric

    2002-05-10

    Acid-sensing ion channels (ASICs) are cationic channels activated by extracellular pH. They are present in the brain, where they are thought to participate in signal transduction associated with local pH variations, and in sensory neurons, where they have been involved in pain perception associated with tissue acidosis and in mechanoperception. The ASIC3 subunit is mainly expressed in dorsal root ganglion neurons. Its expression is associated with a rapidly inactivating current followed by a slowly activating sustained current thought to be required for the tonic sensation of pain caused by acids. We report here the interaction of this channel subunit with the multivalent PDZ (PSD-95 Drosophila discs-large protein, Zonula occludens protein 1) domain-containing protein CIPP. This interaction requires the C-terminal region of ASIC3 and the fourth PDZ domain of CIPP. Co-expression of CIPP and ASIC3 in COS cells increases the maximal ASIC3 peak current density by a factor of 5 and slightly shifts the pH(0.5) for activation from pH 6.2 to pH 6.4. CIPP mRNA is found at a significant level in the same dorsal root ganglion neuronal cell population that expresses the ASIC3 subunit, i.e. mainly in the small nociceptive neurons. CIPP is thus a scaffolding protein that could both enhance the surface expression of ASIC3 and bring together ASIC3 and functionally related proteins in the membrane of sensory neurons.

  20. A Partner's Guide to Pregnancy

    Science.gov (United States)

    ... For Patients About ACOG A Partner's Guide to Pregnancy Home For Patients Search FAQs A Partner's Guide ... May 2016 PDF Format A Partner's Guide to Pregnancy Pregnancy Why is it important to be supportive ...

  1. AgSTAR Partners

    Science.gov (United States)

    AgSTAR’s Partner Program builds stronger relationships with state and non-governmental stakeholders to support all phases of anaerobic digester projects: planning, deployment, and long-term success.

  2. Partners in Pollinator Protection

    Science.gov (United States)

    Partners include USDA, states, international organizations, and stakeholders such as NGOs and bee keepers. Efforts include identifying and using best management practices to reduce honey bee exposure to dust from pesticide-treated seed.

  3. Green Power Partner List

    Science.gov (United States)

    The U.S. EPA's Green Power Partnership is a voluntary program designed to reduce the environmental impact of electricity generation by promoting renewable energy. There are thousands of Green Power Partners, all listed on this page.

  4. Pacific Partners Wargame Analysis

    Science.gov (United States)

    2014-09-01

    participants, U.S. Leaders should 6 Senior Leader Seminars (SLS), Land Forces Talks (LFT), and Executive Steering Groups ( ESG ) are types of senior level...convey information, and enhance strong interpersonal relationships among the current and future senior leaders of the services. LFTs and ESGs are led... ESG focus on the partner nation Army, while LFT generally also include other partner nation security forces. 6

  5. An intriguing shift occurs in the novel protein phosphatase 1 binding partner, TCTEX1D4: evidence of positive selection in a pika model.

    Directory of Open Access Journals (Sweden)

    Luís Korrodi-Gregório

    Full Text Available T-complex testis expressed protein 1 domain containing 4 (TCTEX1D4 contains the canonical phosphoprotein phosphatase 1 (PPP1 binding motif, composed by the amino acid sequence RVSF. We identified and validated the binding of TCTEX1D4 to PPP1 and demonstrated that indeed this protein is a novel PPP1 interacting protein. Analyses of twenty-one mammalian species available in public databases and seven Lagomorpha sequences obtained in this work showed that the PPP1 binding motif 90RVSF93 is present in all of them and is flanked by a palindromic sequence, PLGS, except in three species of pikas (Ochotona princeps, O. dauurica and O. pusilla. Furthermore, for the Ochotona species an extra glycosylation site, motif 96NLS98, and the loss of the palindromic sequence were observed. Comparison with other lagomorphs suggests that this event happened before the Ochotona radiation. The dN/dS for the sequence region comprising the PPP1 binding motif and the flanking palindrome highly supports the hypothesis that for Ochotona species this region has been evolving under positive selection. In addition, mutational screening shows that the ability of pikas TCTEX1D4 to bind to PPP1 is maintained, although the PPP1 binding motif is disrupted, and the N- and C-terminal surrounding residues are also abrogated. These observations suggest pika as an ideal model to study novel PPP1 complexes regulatory mechanisms.

  6. Dataset to delineate changes in association between Akt1 and its interacting partners as a function of active state of Akt1 protein

    Directory of Open Access Journals (Sweden)

    Nutan Gupta

    2017-08-01

    Full Text Available Akt1 is a multi-functional protein, implicated in multiple human solid tumors. Pertaining to its key role in cell survival, Akt1 is under focus for development of targeted therapies. Functional diversity of Akt1 is a result of its interactions with other proteins; which changes with changing context. This investigation was designed to capture the dynamics of Akt1 Interactome as a function of its active state. Delineating dynamic changes in association of Akt1 with its interactors could help us comprehend how it changes as a function of inhibition of its active form. Similar information on changes in Akt1 interactome as of now is not well explored. Akt1 expressing HEK293 cells were cultured in light and heavy labeled SILAC media. Normal lysine and arginine were incorporated as light labels while for heavy labeling the isotopes were 8 and 10 Da heavier. Light labeled cells represented the indigenous state of Akt1 interactome while heavy labeled cells represented Akt1 interactome in presence of its allosteric inhibitor, MK-2206. Equal number of cells from both conditions were pooled, lysed and subjected to Affinity Purification coupled to Mass Spectroscopy (AP-MS. Additionally, SILAC labeling aided in quantitative estimation of changing association of a number of proteins which were common to the two experimental conditions, with Akt1. Data are available via ProteomeXchange with identifier PXD005976.

  7. C1q-tumour necrosis factor-related protein 8 (CTRP8) is a novel interaction partner of relaxin receptor RXFP1 in human brain cancer cells.

    Science.gov (United States)

    Glogowska, Aleksandra; Kunanuvat, Usakorn; Stetefeld, Jörg; Patel, Trushar R; Thanasupawat, Thatchawan; Krcek, Jerry; Weber, Ekkehard; Wong, G William; Del Bigio, Marc R; Hoang-Vu, Cuong; Hombach-Klonisch, Sabine; Klonisch, Thomas

    2013-12-01

    We report a novel ligand-receptor system composed of the leucine-rich G-protein-coupled relaxin receptor, RXFP1, and the C1q-tumour necrosis factor-related protein 8 (CTRP8) in human primary brain cancer, a tumour entity devoid of the classical RXFP1 ligands, RLN1-3. In structural homology studies and computational docking experiments we delineated the N-terminal region of the globular C1q region of CTRP8 and the leucine-rich repeat units 7 and 8 of RXFP1 to mediate this new ligand-receptor interaction. CTRP8 secreted from HEK293T cells, recombinant human (rh) CTRP8, and short synthetic peptides derived from the C1q globular domain of human CTRP8 caused the activation of RXFP1 as determined by elevated intracellular cAMP levels and the induction of a marked pro-migratory phenotype in established glioblastoma (GB) cell lines and primary cells from GB patients. Employing a small competitor peptide, we were able to disrupt the CTRP8-RXFP1-induced increased GB motility. The CTRP8-RXFP1-mediated migration in GB cells involves the activation of PI3K and specific protein kinase C pathways and the increased production/secretion of the potent lysosomal protease cathepsin B (cathB), a known prognostic marker of GB. Specific inhibition of CTRP8-induced cathB activity effectively blocked the ability of primary GB to invade laminin matrices. Finally, co-immunoprecipitation studies revealed the direct interaction of human CTRP8 with RXFP1. Our results support a therapeutic approach in GB aimed at targeting multiple steps of the CTRP8-RXFP1 signalling pathway by a combined inhibitor and peptide-based strategy to block GB dissemination within the brain. Copyright © 2013 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.

  8. Solution Structure of Pfu RPP21, a Component of the Archaeal RNase P Holoenzyme, and Interactions with its RPP29 Protein Partner

    Science.gov (United States)

    Amero, Carlos D; Boomershine, William P; Xu, Yiren; Foster, Mark

    2009-01-01

    RNase P is the ubiquitous ribonucleoprotein metalloenzyme responsible for cleaving the 5′-leader sequence of precursor tRNAs during their maturation. While the RNA subunit is catalytically active on its own at high monovalent and divalent ion concentration, four proteins subunits are associated with archaeal RNase P activity in vivo: RPP21, RPP29, RPP30 and POP5. These proteins have been shown to function in pairs: RPP21-RPP29 and POP5-RPP30. We have determined the solution structure of RPP21 from the hyperthermophilic archaeon Pyrococcus furiosus (Pfu) using conventional and paramagnetic NMR techniques. Pfu RPP21 in solution consists of an unstructured N-terminus, two alpha helices, a zinc binding motif, and an unstructured C-terminus. Moreover, we have used chemical shift perturbations to characterize the interaction of RPP21 with Pfu RPP29. The data show that the primary contact with RPP29 is localized to the two helices of RPP21. This information represents a fundamental step towards understanding structure-function relationships of the archaeal RNase P holoenzyme. PMID:18922021

  9. Solution structure of Pyrococcus furiosus RPP21, a component of the archaeal RNase P holoenzyme, and interactions with its RPP29 protein partner.

    Science.gov (United States)

    Amero, Carlos D; Boomershine, William P; Xu, Yiren; Foster, Mark

    2008-11-11

    RNase P is the ubiquitous ribonucleoprotein metalloenzyme responsible for cleaving the 5'-leader sequence of precursor tRNAs during their maturation. While the RNA subunit is catalytically active on its own at high monovalent and divalent ion concentrations, four protein subunits are associated with archaeal RNase P activity in vivo: RPP21, RPP29, RPP30, and POP5. These proteins have been shown to function in pairs: RPP21-RPP29 and POP5-RPP30. We have determined the solution structure of RPP21 from the hyperthermophilic archaeon Pyrococcus furiosus ( Pfu) using conventional and paramagnetic NMR techniques. Pfu RPP21 in solution consists of an unstructured N-terminus, two alpha-helices, a zinc binding motif, and an unstructured C-terminus. Moreover, we have used chemical shift perturbations to characterize the interaction of RPP21 with RPP29. The data show that the primary contact with RPP29 is localized to the two helices of RPP21. This information represents a fundamental step toward understanding structure-function relationships of the archaeal RNase P holoenzyme.

  10. The journey of integrins and partners in a complex interactions landscape studied by super-resolution microscopy and single protein tracking

    Energy Technology Data Exchange (ETDEWEB)

    Rossier, Olivier; Giannone, Grégory [Univ. Bordeaux, Interdisciplinary Institute for Neuroscience, UMR 5297, F-33000 Bordeaux (France); CNRS, Interdisciplinary Institute for Neuroscience, UMR 5297, F-33000 Bordeaux (France)

    2016-04-10

    Cells adjust their adhesive and cytoskeletal organizations according to changes in the biochemical and physical nature of their surroundings. In return, by adhering and generating forces on the extracellular matrix (ECM) cells organize their microenvironment. Integrin-dependent focal adhesions (FAs) are the converging zones integrating biochemical and biomechanical signals arising from the ECM and the actin cytoskeleton. Thus, integrin-mediated adhesion and mechanotransduction, the conversion of mechanical forces into biochemical signals, are involved in critical cellular functions such as migration, proliferation and differentiation, and their deregulation contributes to pathologies including cancer. A challenging problem is to decipher how stochastic protein movements and interactions lead to formation of dynamic architecture such as integrin-dependent adhesive structures. In this review, we will describe recent advances made possible by super-resolution microscopies and single molecule tracking approaches that provided new understanding on the organization and the dynamics of integrins and intracellular regulators at the nanoscale in living cells.

  11. Intimate partner violence (IPV)

    DEFF Research Database (Denmark)

    Rasch, Vibeke; Van, Toan Ngo; Nguyen, Hanh Thi Thuy

    2018-01-01

    BACKGROUND: Intimate partner violence (IPV) is a global problem that affects one-third of all women. The present study aims to develop and determine the validity of a screening instrument for the detection of IPV in pregnant women in Tanzania and Vietnam and to determine the minimum number...

  12. Predictability of Conversation Partners

    Directory of Open Access Journals (Sweden)

    Taro Takaguchi

    2011-09-01

    Full Text Available Recent developments in sensing technologies have enabled us to examine the nature of human social behavior in greater detail. By applying an information-theoretic method to the spatiotemporal data of cell-phone locations, [C. Song et al., Science 327, 1018 (2010SCIEAS0036-8075] found that human mobility patterns are remarkably predictable. Inspired by their work, we address a similar predictability question in a different kind of human social activity: conversation events. The predictability in the sequence of one’s conversation partners is defined as the degree to which one’s next conversation partner can be predicted given the current partner. We quantify this predictability by using the mutual information. We examine the predictability of conversation events for each individual using the longitudinal data of face-to-face interactions collected from two company offices in Japan. Each subject wears a name tag equipped with an infrared sensor node, and conversation events are marked when signals are exchanged between sensor nodes in close proximity. We find that the conversation events are predictable to a certain extent; knowing the current partner decreases the uncertainty about the next partner by 28.4% on average. Much of the predictability is explained by long-tailed distributions of interevent intervals. However, a predictability also exists in the data, apart from the contribution of their long-tailed nature. In addition, an individual’s predictability is correlated with the position of the individual in the static social network derived from the data. Individuals confined in a community—in the sense of an abundance of surrounding triangles—tend to have low predictability, and those bridging different communities tend to have high predictability.

  13. Predictability of Conversation Partners

    Science.gov (United States)

    Takaguchi, Taro; Nakamura, Mitsuhiro; Sato, Nobuo; Yano, Kazuo; Masuda, Naoki

    2011-08-01

    Recent developments in sensing technologies have enabled us to examine the nature of human social behavior in greater detail. By applying an information-theoretic method to the spatiotemporal data of cell-phone locations, [C. Song , ScienceSCIEAS0036-8075 327, 1018 (2010)] found that human mobility patterns are remarkably predictable. Inspired by their work, we address a similar predictability question in a different kind of human social activity: conversation events. The predictability in the sequence of one’s conversation partners is defined as the degree to which one’s next conversation partner can be predicted given the current partner. We quantify this predictability by using the mutual information. We examine the predictability of conversation events for each individual using the longitudinal data of face-to-face interactions collected from two company offices in Japan. Each subject wears a name tag equipped with an infrared sensor node, and conversation events are marked when signals are exchanged between sensor nodes in close proximity. We find that the conversation events are predictable to a certain extent; knowing the current partner decreases the uncertainty about the next partner by 28.4% on average. Much of the predictability is explained by long-tailed distributions of interevent intervals. However, a predictability also exists in the data, apart from the contribution of their long-tailed nature. In addition, an individual’s predictability is correlated with the position of the individual in the static social network derived from the data. Individuals confined in a community—in the sense of an abundance of surrounding triangles—tend to have low predictability, and those bridging different communities tend to have high predictability.

  14. Parents as Writing Partners

    Science.gov (United States)

    Ehrenworth, Mary

    2014-01-01

    Parents know that writing is essential to their children's success, and they're eager to help their children become good writers. But often, they're at a loss about how to help. Instead of leaving them in the dark, schools can make parents into valuable writing partners by giving them a toolkit of guidelines for coaching writers.…

  15. Partnering i praksis

    DEFF Research Database (Denmark)

    Gottlieb, Stefan Christoffer; Bang, Henrik L.; Larsen, Jacob Norvig

    Publikationen sammenfatter resultater og erfaringer vedrørende brug af partnering i realiseringen af LO's hovedsæde i Havnestaden, København. Forløbet i byggesagen adskiller sig fra traditionelle byggesager på en række områder. Vigtigst af disse er anvendelsen af en række partneringstiltag, der...... udgør et brud med den traditionelle organisering og gennemførelse af byggesager. Dog har det været vanskeligt at realisere intentionerne om det gode samarbejde. Anvendelsen af partnering har således ikke resulteret i ændret arbejdspraksis. Som årsager hertil peges bl.a. på, at byggeriets traditionelle...... kontraktlige aftalesystem står i modsætningsforhold til de grundlæggende forudsætninger for partnering, og at der mangler operationelle værktøjer til understøttelse af partneringsprocessen....

  16. Identification of nucleosome assembly protein 1 (NAP1) as an interacting partner of plant ribosomal protein S6 (RPS6) and a positive regulator of rDNA transcription

    Energy Technology Data Exchange (ETDEWEB)

    Son, Ora [Department of Biological Science, Sookmyung Women' s University, Seoul 140-742 (Korea, Republic of); Kim, Sunghan [Department of Biological Science, Sookmyung Women' s University, Seoul 140-742 (Korea, Republic of); Department of Plant Science, Plant Genomics and Breeding Institute, Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 151-921 (Korea, Republic of); Shin, Yun-jeong [Department of Biological Science, Sookmyung Women' s University, Seoul 140-742 (Korea, Republic of); Kim, Woo-Young [College of Pharmacy, Sookmyung Women' s University, Seoul 140-742 (Korea, Republic of); Koh, Hee-Jong, E-mail: heejkoh@snu.ac.kr [Department of Plant Science, Plant Genomics and Breeding Institute, Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 151-921 (Korea, Republic of); Cheon, Choong-Ill, E-mail: ccheon@sookmyung.ac.kr [Department of Biological Science, Sookmyung Women' s University, Seoul 140-742 (Korea, Republic of)

    2015-09-18

    The ribosomal protein S6 (RPS6) is a downstream component of the signaling mediated by the target of rapamycin (TOR) kinase that acts as a central regulator of the key metabolic processes, such as protein translation and ribosome biogenesis, in response to various environmental cues. In our previous study, we identified a novel role of plant RPS6, which negatively regulates rDNA transcription, forming a complex with a plant-specific histone deacetylase, AtHD2B. Here we report that the Arabidopsis RPS6 interacts additionally with a histone chaperone, nucleosome assembly protein 1(AtNAP1;1). The interaction does not appear to preclude the association of RPS6 with AtHD2B, as the AtNAP1 was also able to interact with AtHD2B as well as with an RPS6-AtHD2B fusion protein in the BiFC assay and pulldown experiment. Similar to a positive effect of the ribosomal S6 kinase 1 (AtS6K1) on rDNA transcription observed in this study, overexpression or down regulation of the AtNAP1;1 resulted in concomitant increase and decrease, respectively, in rDNA transcription suggesting a positive regulatory role played by AtNAP1 in plant rDNA transcription, possibly through derepression of the negative effect of the RPS6-AtHD2B complex. - Highlights: • Nucleosome assembly protein 1 (AtNAP1) interacts with RPS6 as well as with AtHD2B. • rDNA transcription is regulated S6K1. • Overexpression or down regulation of AtNAP1 results in concomitant increase or decrease in rDNA transcription.

  17. Partner personality in distressed relationships

    NARCIS (Netherlands)

    Barelds, D.P.H.; Barelds-Dijkstra, P.

    2006-01-01

    The present study examines the personality characteristics of partners receiving marital therapy. On the basis of previous research, we expected partners in distressed relationships to be more neurotic and more introverted and to have lower self-esteem than partners in non-distressed relationships.

  18. Nucleotide Excision Repair Lesion-Recognition Protein Rad4 Captures a Pre-Flipped Partner Base in a Benzo[a]pyrene-Derived DNA Lesion: How Structure Impacts the Binding Pathway.

    Science.gov (United States)

    Mu, Hong; Geacintov, Nicholas E; Min, Jung-Hyun; Zhang, Yingkai; Broyde, Suse

    2017-06-19

    The xeroderma pigmentosum C protein complex (XPC) recognizes a variety of environmentally induced DNA lesions and is the key in initiating their repair by the nucleotide excision repair (NER) pathway. When bound to a lesion, XPC flips two nucleotide pairs that include the lesion out of the DNA duplex, yielding a productively bound complex that can lead to successful lesion excision. Interestingly, the efficiencies of NER vary greatly among different lesions, influencing their toxicity and mutagenicity in cells. Though differences in XPC binding may influence NER efficiency, it is not understood whether XPC utilizes different mechanisms to achieve productive binding with different lesions. Here, we investigated the well-repaired 10R-(+)-cis-anti-benzo[a]pyrene-N 2 -dG (cis-B[a]P-dG) DNA adduct in a duplex containing normal partner C opposite the lesion. This adduct is derived from the environmental pro-carcinogen benzo[a]pyrene and is likely to be encountered by NER in the cell. We have extensively investigated its binding to the yeast XPC orthologue, Rad4, using umbrella sampling with restrained molecular dynamics simulations and free energy calculations. The NMR solution structure of this lesion in duplex DNA has shown that the dC complementary to the adducted dG is flipped out of the DNA duplex in the absence of XPC. However, it is not known whether the "pre-flipped" base would play a role in its recognition by XPC. Our results show that Rad4 first captures the displaced dC, which is followed by a tightly coupled lesion-extruding pathway for productive binding. This binding path differs significantly from the one deduced for the small cis-syn cyclobutane pyrimidine dimer lesion opposite mismatched thymines [ Mu , H. , ( 2015 ) Biochemistry , 54 ( 34 ), 5263 - 7 ]. The possibility of multiple paths that lead to productive binding to XPC is consistent with the versatile lesion recognition by XPC that is required for successful NER.

  19. New partner - Forestland

    CERN Multimedia

    Staff Association

    2014-01-01

      New partner   Do you need a moment of relaxation and adventure?  Come to Divonne-les-bains and benefit of an immediate discount of 20% on all ropes courses of Forestland http://www.forestland.fr/ upon presentation of your Staff Association membership card. The park is open from 10h00 to 19h00 on Wednesday, Saturday, Sunday during the school period and public holidays (France and Switzerland) or every day during school holidays (France and Switzerland). Different levels of difficulty are available: children, juniors, adults, athletes.

  20. The constitution of partnering

    DEFF Research Database (Denmark)

    Gottlieb, Stefan Christoffer

    it is demonstrated how the newly established notion of the construction sector, founded on a technical-scientific rationality, gave rise to a pervasive functional differentiation, in which existing practices, material and actors was subjected to a gaze of normation owing to the normative ideal of the optimal...... place in the efforts to handle the tensions between a traditional stratified sociality, with its commonsense qualities, and a 'smooth' sociality in which circulation, self-governance, individual responsibility and ownership are more important than unambiguity, planning and control. Furthermore......This dissertation has been submitted in partial requirements for the PhD degree at the Technical University of Denmark. The dissertation is the result of a study entitled 'Interorganisational knowledge processes in construction. Knowledge and practice in partnering.' The study has been conducted...

  1. Partnering with NASA: An Overview

    Science.gov (United States)

    Martin, Gary

    2017-01-01

    Partnerships is an important part of doing business at NASA. NASA partners with external organizations to access capabilities under collaborative agreements; enters into agreements for partner access to NASA capabilities; expand overall landscape of space activity; and spurring innovation. NASA partnerships consist of Reimbursable and Non-Reimbursable Space Act Agreements. Partnerships at Ames aligns with Ames' core competencies, and Partners often office in the NASA Research Park, which is an established regional innovation cluster that facilitates commercialization and services as a technology accelerator via onsite collaborations between NASA and its partners.

  2. Partners' relationship quality and childbearing

    NARCIS (Netherlands)

    Rijken, A.J.; Thomson, E.

    2011-01-01

    This study examines the influence of partner relationship quality on childbearing. We are innovative in using relationship quality reports from both partners, drawing on the first and second wave of the Netherlands Kinship Panel Study. Thus, we can identify potential effects of discordant

  3. Partner selection and Hollywood Films

    DEFF Research Database (Denmark)

    Grodal, Torben Kragh; Kramer, Mette

    2012-01-01

    Based on cognitive, neurological and evolutionary based film theory the article describes the representation of partner selection in Hollywood films. It analyses paradigm scenarios of partner selection and love, It further describes some of those mechanisms that regulate the relation between...

  4. Partnering for Success (OIT Customer Day Partner Recognition)

    Energy Technology Data Exchange (ETDEWEB)

    2002-04-01

    Office of Industrial Technologies document produced for 2002 Customer Day event, which features industry partners who have worked with OIT to achieve outstanding energy efficiency achievements from January 2001 to the present.

  5. Flexible nets: disorder and induced fit in the associations of p53 and 14-3-3 with their partners

    OpenAIRE

    Uversky Vladimir N; Yang Mary Qu; Yang Jack Y; Meng Jingwei; Oldfield Christopher J; Dunker A Keith

    2008-01-01

    Abstract Background Proteins are involved in many interactions with other proteins leading to networks that regulate and control a wide variety of physiological processes. Some of these proteins, called hub proteins or hubs, bind to many different protein partners. Protein intrinsic disorder, via diversity arising from structural plasticity or flexibility, provide a means for hubs to associate with many partners (Dunker AK, Cortese MS, Romero P, Iakoucheva LM, Uversky VN: Flexible Nets: The r...

  6. Become a Green Power Partner

    Science.gov (United States)

    The U.S. EPA's Green Power Partnership is a voluntary program designed to reduce the environmental impact of electricity generation by promoting renewable energy. Learn more about becoming a Green Power Partner, including recognition opportunities.

  7. Partners Against Crime (PAC) Districts

    Data.gov (United States)

    City and County of Durham, North Carolina — The Partners Against Crime (PAC) program promotes collaboration among police officers, Durham residents, and city and county government officials to find...

  8. Partnering models in Nordic construction

    DEFF Research Database (Denmark)

    Larsen, Jacob Norvig

    Traditionally, procurement and contractual policies adopted by building and construction clients produce a system in which clients procure design services separately from construction services, while operation and maintenance have been subject to further, separate procurement actions...... delivery, often in combination with the introduction of private finance in hitherto publicly funded buildings and infrastructure works (PPP, PFI). Some construction clients have taken this a step further and adopted a much more collaborative approach towards project delivery, often known as partnering...... of local research and industry partners including major building clients. Data were collected by means of national reviews of partnering policies and practices, thematic analyses, and case studies. The concept partnering was introduced in a Nordic context in the 1990s and has since then been implemented...

  9. Partnering in the Construction Industry

    DEFF Research Database (Denmark)

    Ditlev Bohnstedt, Kristian; Bejder, Erik; Haugbølle, Kim

    2013-01-01

    Huge efforts have been made in order to stimulate thorough improvements in the construction industry in terms of value for money, feasibility and consistency when facilitating a partnering approach. Despite this attention there is limited documentation on the development of research trends, thus...... gaps in existing body of knowledge. The objective of this literature-based paper is to explore the current literature by systematically reviewing and summarizing research trends in leading top-tier construction management journals between 2002 and 2012, addressing how the themes in partnering related...... publications change across this period. It is concluded that trends such as research on developing trust among construction partners to facilitate project success, feasibility of and the barriers to adopting a partnering approach and strategic propositions for overcoming barriers are highly emerging. Finally...

  10. Partner reaction following ostomy surgery.

    Science.gov (United States)

    Gloeckner, M R

    1983-01-01

    This study was conducted to obtain information regarding sexual adjustment following ostomy surgery and to explore the spouse/partner's reaction upon resuming sexual activities following surgery. Home interviews were conducted with 40 subjects who had a permanent stoma, where the mean length of time since surgery averaged 4.6 years. Retrospective perceptions of the spouse/partner's reactions were varied. A majority reported that their spouses reacted positively to the first sexual experience following ostomy surgery. However a substantial number of subjects stated that their sexual partner reacted with caution ("fear of hurting me") or in a negative manner. This and previous studies indicate that the sexual partner plays a key role in helping the person adjust following ostomy surgery.

  11. Same-Sex Partner Bereavement.

    Science.gov (United States)

    Patlamazoglou, Lefteris; Simmonds, Janette G; Snell, Tristan L

    2017-01-01

    The experience of same-sex-attracted people who have lost a partner is neglected in the existing literature on bereavement. Previous research on lesbian, gay, bisexual, transgender, intersex, queer and questioning (LGBTIQ) populations tends to focus on the loss of a partner to HIV-related causes, and there is scant research concerning non-HIV-related bereavement. The purpose of this article is to investigate the non-HIV-related bereavement experiences of same-sex partners and to address the potential complications of disenfranchised grief. Coping with the loss of a same-sex partner and the impact of bereavement on subsequent relationships are also discussed. Implications for counseling of bereaved same-sex-attracted individuals are drawn, and recommendations for future psychological research on the experience of bereavement are made.

  12. Partner Ballroom Dance Robot -PBDR-

    Science.gov (United States)

    Kosuge, Kazuhiro; Takeda, Takahiro; Hirata, Yasuhisa; Endo, Mitsuru; Nomura, Minoru; Sakai, Kazuhisa; Koizumi, Mizuo; Oconogi, Tatsuya

    In this research, we have developed a dance partner robot, which has been developed as a platform for realizing the effective human-robot coordination with physical interaction. The robot could estimate the next dance step intended by a human and dance the step with the human. This paper introduce the robot referred to as PBDR (Partner Ballroom Dance Robot), which has performed graceful dancing with the human in EXPO 2005, Aichi, Japan.

  13. Partner violence and abortion characteristics.

    Science.gov (United States)

    Colarossi, Lisa; Dean, Gillian

    2014-01-01

    We conducted a retrospective cohort study using randomly selected medical charts of women reporting a history of partner violence and women with no history of partner violence at the time of a family planning or abortion appointment (n = 6,564 per group). We analyzed lifetime history of partner violence for odds of lifetime history of abortion and miscarriage number, and birth control problems. To more closely match timing, we analyzed a subsample of 2,186 women reporting current violence versus not at the time of an abortion appointment for differences in gestational age, medical versus surgical method choice, and return for follow-up visit. After adjusting for years at risk and demographic characteristics, women with a past history of partner violence were not more likely to have ever had one abortion, but they were more likely to have had problems with birth control, repeat abortions, and miscarriages than women with no history of violence. Women with current partner violence were also more likely to be receiving an abortion at a later gestational age. We found no differences between the groups in return for abortion follow-up visit or choice of surgical versus medication abortion. Findings support screening for the influence of partner violence on reproductive health and related safety planning.

  14. Publishing protocols for partnered research.

    Science.gov (United States)

    Hysong, Sylvia J; Woodard, LeChauncy; Garvin, Jennifer H; Murawsky, Jeffrey; Petersen, Laura A

    2014-12-01

    Published scientific protocols are advocated as a means of controlling bias in research reporting. Indeed, many journals require a study protocol with manuscript submission. However, publishing protocols of partnered research (PPR) can be challenging in light of the research model's dynamic nature, especially as no current reporting standards exist. Nevertheless, as these protocols become more prevalent, a priori documentation of methods in partnered research studies becomes increasingly important. Using as illustration a suite of studies aimed at improving coordination and communication in the primary care setting, we sought to identify challenges in publishing PPR relative to traditional designs, present alternative solutions to PPR publication, and propose an initial checklist of content to be included in protocols of partnered research. Challenges to publishing PPR include reporting details of research components intended to be co-created with operational partners, changes to sampling and entry strategy, and alignment of scientific and operational goals. Proposed solutions include emulating reporting standards of qualitative research, participatory action research, and adaptive trial designs, as well as embracing technological tools that facilitate publishing adaptive protocols, with version histories that are able to be updated as major protocol changes occur. Finally, we present a proposed checklist of reporting elements for partnered research protocols.

  15. Intimate partner violence at a tertiary institution

    African Journals Online (AJOL)

    or threatened physical, sexual, psychological, emotional, or stalking abuse by an intimate partner'. An intimate partner can be a current or former spouse or a nonmarital partner, such as a boyfriend, girlfriend or dating partner, and can be someone of the same or the opposite sex. The South African (SA) Domestic Violence ...

  16. Pharma partnering: other people's science.

    Science.gov (United States)

    Hofmann, Christian

    2017-01-01

    Partnering is an ideal field if someone is seeking to move from a scientific to a more business-oriented discipline. Partnering's goal is to identify and acquire external innovation. These discoveries are then included in a company's pipeline and help bring novel treatments to patients. Advanced scientific training is essential in the identification and evaluation of these external assets. Here I describe how partnering works in a pharmaceutical company and offer advice on how to make a successful transition from a PhD program to a business career. © 2017 Hofmann. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

  17. Partnering and integrated supply management

    DEFF Research Database (Denmark)

    Bjarnø, Ole-Christian; Olsen, Anders; Thyssen, Mikael

    2003-01-01

    are common among the actors in the building industry. Partnering is still in its early stages, and a strategic development of the concept to include long-term collaborative relationships appears to be able to offer considerable potential, while there is a pressing need for the development of methods...... for strategic management of collaborative relationships on a line with the purchasing perspectives offered by Supply Chain Management. Based on a study of the literature and an in-depth case study carried out within a large Scandinavian contractor, this article gives a proposal for how Partnering can...... be supported by strategic purchasing, with the aim of achieving strategic Partnering. The contribution of this article is thus the development of a new purchasing perspective within Construction Supply Chain Management....

  18. Partnering for Student Transfer Success

    Science.gov (United States)

    Washington State Board for Community and Technical Colleges, 2015

    2015-01-01

    Washington's community and technical colleges, private non-profit baccalaureate, and public baccalaureate colleges and universities have a proud history of partnering to serve students. Nowhere is this cooperation more evident than in the smooth transfer process from community and technical colleges into four-year colleges and universities. This…

  19. Validation of Fear of Partner Scale.

    Science.gov (United States)

    O'Leary, K Daniel; Foran, Heather; Cohen, Shiri

    2013-10-01

    Therapists have a responsibility to ascertain if psychological aggression, physical aggression, sexual aggression exist, and if there is fear of the partner. A fear of partner measure was evaluated in 100 couples who sought relationship feedback. Fear of partner's psychological, physical, and sexual aggression was related to actual reports of such behavior. For both men and women, fear of speaking in front of partner and fear of being in therapy with partner were related to reports of psychological aggression perpetrated by the partner, dominance, and isolation by the partner, and one's own marital dissatisfaction. Among respondents who were aggressed against, more men than women reported fear of participating in therapy with their partner. The measure herein can be used to determine the extent of fear of aggression by partners and to assist in the decision-making about the appropriateness of marital therapy and divorce mediation. © 2013 American Association for Marriage and Family Therapy.

  20. With or without you: does partner satisfaction and partner-directed violence influence the presence of a partner on women's Facebook cover profile photographs?

    National Research Council Canada - National Science Library

    Pavol Prokop; Natália Morvayová

    2016-01-01

    .... We investigated relationships between the presence of a current romantic partner on the CPP and the displayed relationship status and partner satisfaction, partner-directed violence and women's...

  1. Genital Herpes in Marital Partners

    Directory of Open Access Journals (Sweden)

    Mary Jacob

    1988-01-01

    Full Text Available During 1983-86, 225 patients were clinically diagnosed to have genital herpes (GH at our clinic. Of these, 90 men and 55 women were currently married. All the spouses were screened clinically and through standardized techniques for isolation and typing of herpes simplex virus, serological testing and Papanicolaou smear. There were 90 couples in whom at least one spouse had GH and in 38 (42% couples both partners had GH. Clinically, 49% of wives and 75% of husbands of GH patients were diagnosed to have the disease. The spouses of recurrent GH patients had a higher frequency of the disease than spouses of primary GH patients. Among spouses who were clinically asymptomatic, 40% had high serological titres suggestive of GH. Wives generally experienced more severe symptoms, especially pain in the lesions. Majority of lesions in both the partners were vesicles and ulcers. Prodromata were more among recurrent GH patients in both the partners. The frequency of recurrences wasalso similar in spouses. Seventy percent of wives and 40% of husbands could not identify any precipitating factor. Intercourse, physical stress and rich food were cited as possible factors in the remaining. All the wives had acquired the diseases through their husbands who were promiscuous. Fifty percent of husbands had been infected before marriage. Given the fact that asymptomatic carriers exist, it is better to consider all marital partners of GH as infected. Repeated and long-term follow, - up examination, particularly of wives of GH patients is therefore essential as an important socio-preventive aspect of this disease.

  2. Parents, Reading Partners, Library Advocates

    Science.gov (United States)

    Deskins, Liz

    2011-01-01

    According to the author, one of her most important goals as a school librarian is to inspire her students to become lifelong lovers of reading. She recognizes that she cannot do this alone, and one of her most powerful partners is a parent. She can encourage a child to check out a book that may open their eyes to the wonders of literature, but if…

  3. Intimate Partner Violence, 1993-2010

    Science.gov (United States)

    ... 2015 Special Report NCJ 2392 03 Intimate Partner Violence, 1993–2010 Shannan Catalano, Ph.D., BJS Statistician ... to 2010, the overall rate of intimate partner violence in the United States declined by 64%, from ...

  4. Bed Partner Often Fuels Loved One's Insomnia

    Science.gov (United States)

    ... 166425.html Bed Partner Often Fuels Loved One's Insomnia Well-intentioned tips to encourage better sleep often ... specialists found that when a loved one had insomnia, the partner's suggested solutions -- including watching TV or ...

  5. Million Solar Roofs: Partners Make Markets

    Energy Technology Data Exchange (ETDEWEB)

    2004-06-01

    Million Solar Roofs (MSR) Partners Make Markets Executive Summary is a summary of the MSR Annual Partnership Update, a report from all the partners and partnerships who participate in the MSR Initiative.

  6. Dynamic hubs show competitive and static hubs non-competitive regulation of their interaction partners.

    Directory of Open Access Journals (Sweden)

    Apurv Goel

    Full Text Available Date hub proteins have 1 or 2 interaction interfaces but many interaction partners. This raises the question of whether all partner proteins compete for the interaction interface of the hub or if the cell carefully regulates aspects of this process? Here, we have used real-time rendering of protein interaction networks to analyse the interactions of all the 1 or 2 interface hubs of Saccharomyces cerevisiae during the cell cycle. By integrating previously determined structural and gene expression data, and visually hiding the nodes (proteins and their edges (interactions during their troughs of expression, we predict when interactions of hubs and their partners are likely to exist. This revealed that 20 out of all 36 one- or two- interface hubs in the yeast interactome fell within two main groups. The first was dynamic hubs with static partners, which can be considered as 'competitive hubs'. Their interaction partners will compete for the interaction interface of the hub and the success of any interaction will be dictated by the kinetics of interaction (abundance and affinity and subcellular localisation. The second was static hubs with dynamic partners, which we term 'non-competitive hubs'. Regulatory mechanisms are finely tuned to lessen the presence and/or effects of competition between the interaction partners of the hub. It is possible that these regulatory processes may also be used by the cell for the regulation of other, non-cell cycle processes.

  7. Actors', partners', and observers' perceptions of sarcasm.

    Science.gov (United States)

    Rockwell, P

    2000-10-01

    This study compared actors', partners', and observers' perceptions of the amount of sarcasm used by participants (n = 80) in videotaped conversations. Significant differences were found among perceptions of actors, partners, and observers. Of the three perspectives, actors perceived themselves as using the greatest amount of sarcasm, followed by partners' perceptions of actors. Observers perceived actors as using the least amount of sarcasm. Correlations conducted to assess whether partners and observers recognized actors' individual attempts at sarcasm during the conversations were generally low.

  8. Determinants of communication between partners about STD ...

    African Journals Online (AJOL)

    Determinants of communication between partners about STD symptoms: implications for partner referral in South Africa. ... to have good knowledge about the effects of STDs and the transmission of STDs in the absence of symptoms, had positive attitudes towards condoms and perceived social support for partner referral.

  9. Living with heart failure : Partner perspectives

    NARCIS (Netherlands)

    Luttik, Marie Louise; Blaauwbroek, Arnarins; Dijker, Anton; Jaarsma, Tiny

    2007-01-01

    To preserve the supportive capabilities of partners of heart failure (HF) patients, it is necessary to gain insight in the experiences and potential needs of these partners. Thirteen partners of HF patients participated in semistructured interviews specifically focused on their experiences as a

  10. Positive illusions about one's partner's physical attractiveness

    NARCIS (Netherlands)

    Barelds-Dijkstra, Pieternel; Barelds, Dick P. H.

    This study examined couples' ratings of self and partner physical attractiveness. On the basis of the theory of positive illusions, it was expected that individuals would rate their partners as more attractive than their partners would rate themselves. Both members of 93 heterosexual couples, with a

  11. Partner Stalking and Implications for Women's Employment

    Science.gov (United States)

    Logan, TK; Shannon, Lisa; Cole, Jennifer; Swanberg, Jennifer

    2007-01-01

    In general research suggests partner violence has a negative impact on women's employment. However, there has been limited examination of partner stalking and consequences for employment. The purpose of this study was to examine partner stalking and employment consequences among two samples of women. One sample was women who had obtained a…

  12. "Program for Partners": Support Groups for Partners of Adults with Visual Impairments

    Science.gov (United States)

    Cimarolli, Verena; Sussman-Skalka, Carol; Goodman, Caryn

    2004-01-01

    This study of time-limited support groups attended by partners of individuals with visual impairments found that participation increased the attendees' knowledge of their visually impaired partners' situations, improved the quality of communication between the partners, and reduced the sighted partners' negative appraisal of their role.

  13. Maternal Re-Partnering and New-Partner Fertility: Associations with Nonresident Father Investments in Children.

    Science.gov (United States)

    Berger, Lawrence M; Cancian, Maria; Meyer, Daniel R

    2012-02-01

    Research suggests that paternal re-partnering and new-partner fertility are associated with decreased nonresident father investments in children. Few studies, however, have examined the influence of maternal re-partnering and new-partner births on nonresident father investments. We use data from the National Longitudinal Survey of Youth to examine associations of maternal re-partnering (through cohabitation or marriage with a new partner) and new-partner births with nonresident father visitation and child support payments. Results suggest that maternal re-partnering is associated with a decrease in both yearly father-child contact and child support received by the mother. New-partner fertility for mothers who are co-residing with a partner is associated with an additional decrease in monthly father-child contact, but does not have an additional influence on yearly father-child contact or child support receipt.

  14. GPCR & company: databases and servers for GPCRs and interacting partners.

    Science.gov (United States)

    Kowalsman, Noga; Niv, Masha Y

    2014-01-01

    G-protein-coupled receptors (GPCRs) are a large superfamily of membrane receptors that are involved in a wide range of signaling pathways. To fulfill their tasks, GPCRs interact with a variety of partners, including small molecules, lipids and proteins. They are accompanied by different proteins during all phases of their life cycle. Therefore, GPCR interactions with their partners are of great interest in basic cell-signaling research and in drug discovery.Due to the rapid development of computers and internet communication, knowledge and data can be easily shared within the worldwide research community via freely available databases and servers. These provide an abundance of biological, chemical and pharmacological information.This chapter describes the available web resources for investigating GPCR interactions. We review about 40 freely available databases and servers, and provide a few sentences about the essence and the data they supply. For simplification, the databases and servers were grouped under the following topics: general GPCR-ligand interactions; particular families of GPCRs and their ligands; GPCR oligomerization; GPCR interactions with intracellular partners; and structural information on GPCRs. In conclusion, a multitude of useful tools are currently available. Summary tables are provided to ease navigation between the numerous and partially overlapping resources. Suggestions for future enhancements of the online tools include the addition of links from general to specialized databases and enabling usage of user-supplied template for GPCR structural modeling.

  15. To punish or to leave: distinct cognitive processes underlie partner control and partner choice behaviors.

    Directory of Open Access Journals (Sweden)

    Justin W Martin

    Full Text Available When a cooperative partner defects, at least two types of response are available: Punishment, aimed at modifying behavior, and ostracism, aimed at avoiding further social interaction with the partner. These options, termed partner control and partner choice, have been distinguished at behavioral and evolutionary levels. However, little work has compared their cognitive bases. Do these disparate behaviors depend on common processes of moral evaluation? Specifically, we assess whether they show identical patterns of dependence on two key dimensions of moral evaluation: A person's intentions, and the outcomes that they cause. We address this issue in a "trembling hand" economic game. In this game, an allocator divides a monetary stake between themselves and a responder based on a stochastic mechanism. This allows for dissociations between the allocator's intent and the actual outcome. Responders were either given the opportunity to punish or reward the allocator (partner control or to switch to a different partner for a subsequent round of play (partner choice. Our results suggest that partner control and partner choice behaviors are supported by distinct underlying cognitive processes: Partner control exhibits greater sensitivity to the outcomes a partner causes, while partner choice is influenced almost exclusively by a partner's intentions. This cognitive dissociation can be understood in light of the unique adaptive functions of partner control and partner choice.

  16. Prioritizing partners across the continuum.

    Science.gov (United States)

    Maly, Mary Beth; Lawrence, Susan; Jordan, M Kim; Davies, William J; Weiss, Michael J; Deitrick, Lynn; Salas-Lopez, Debbie

    2012-11-01

    With the advent of accountable care organizations, bundled payments, value-based purchasing, and penalties for preventable hospital readmission, tight connections and collaboration across the care continuum will become critical to achieve successful patient outcomes and to reduce the cost of care delivery. Lehigh Valley Health Network (LVHN), the largest provider of health services in eastern Pennsylvania, set out on a journey to build collaborative relationships with skilled nursing facilities (SNFs) in their eastern Pennsylvania community. LVHN desired SNF partners with mutual interests in improving quality of care and lowering costs of delivery where possible. Recognizing that not all SNFs are alike, LVHN developed a Collaborative Partner Prioritization Tool to assess and prioritize skilled nursing facilities in an effort to determine those that would make the best collaborators. SNFs were reviewed based on their volume of mutual patients, quality of care delivery, and their perceived willingness to align with LVHN. Six variables were used to assess these facilities, including (1) patient discharge destination volume by SNF; (2) 30-day all-cause readmission rate to an LVHN hospital; (3) Medicare's Nursing Home Compare 5-Star Overall Rating; (4) the health network affiliation of the SNF's medical director; (5) the level of LVHN-employed or -affiliated physician presence at the SNF; and (6) the SNF's current participation in LVHN-sponsored programs and meetings. Through use of the Collaborative Partner Prioritization Tool, it was discovered that roughly 70% of LVHN patients who required skilled nursing care following their inpatient stay received care at 1 of 20 SNFs. Of these, 5 facilities performed well on the 6-variable assessment, deeming them the "Tier 1 Facilities" to initially focus collaborative efforts. LVHN has strategically deployed physician resources and has increased physician presence at these "Tier 1 SNFs." These facilities have also gained remote

  17. Partner notification in the management of sexually transmitted ...

    African Journals Online (AJOL)

    Barriers to partner notification included partners being out of town (44.6%) fear of quarrels and violence from partners (32.5%) and casual partners (15.1%) whose sex partners were unknown. Conclusion: Counseling and understanding of STIs patients on the need to treat all sexual partners is pivotal to the success of ...

  18. Intimate partner violence in African American women.

    Science.gov (United States)

    Campbell, Doris Williams; Sharps, Phyllis W; Gary, Faye A; Campbell, Jacquelyn C; Lopez, Loretta M

    2002-01-01

    Violence against African American women, specifically intimate partner abuse, has a significant impact on their health and well being. Intimate partner femicide and near fatal intimate partner femicide are the major causes of premature death and disabling injuries for African American women. Yet, despite this, there is a paucity of research and interventions specific and culturally relevant for these women. This article focuses on issues relevant to intimate partner violence and abuse against African American women by examining existing empirical studies of prevalence and health outcomes of intimate partner violence against women in general, plus what limited research there is about African American women, specifically. It includes a discussion of specific recommendations for research, practice, education, and policy to reduce and prevent intimate partner violence against African American women.

  19. Find Recycling Facilities Servicing RAD Partners

    Science.gov (United States)

    RAD partners help protect the ozone layer and reduce emissions of greenhouse gases by disposing of older, inefficient refrigerated appliances using the best environmental practices and technologies available.

  20. Small Heterodimer Partner and Innate Immune Regulation

    Directory of Open Access Journals (Sweden)

    Jae-Min Yuk

    2016-03-01

    Full Text Available The nuclear receptor superfamily consists of the steroid and non-steroid hormone receptors and the orphan nuclear receptors. Small heterodimer partner (SHP is an orphan family nuclear receptor that plays an essential role in the regulation of glucose and cholesterol metabolism. Recent studies reported a previously unidentified role for SHP in the regulation of innate immunity and inflammation. The innate immune system has a critical function in the initial response against a variety of microbial and danger signals. Activation of the innate immune response results in the induction of inflammatory cytokines and chemokines to promote anti-microbial effects. An excessive or uncontrolled inflammatory response is potentially harmful to the host, and can cause tissue damage or pathological threat. Therefore, the innate immune response should be tightly regulated to enhance host defense while preventing unwanted immune pathologic responses. In this review, we discuss recent studies showing that SHP is involved in the negative regulation of toll-like receptor-induced and NLRP3 (NACHT, LRR and PYD domains-containing protein 3-mediated inflammatory responses in innate immune cells. Understanding the function of SHP in innate immune cells will allow us to prevent or modulate acute and chronic inflammation processes in cases where dysregulated innate immune activation results in damage to normal tissues.

  1. Involving Support Partners in Obesity Treatment

    Science.gov (United States)

    Gorin, Amy; Phelan, Suzanne; Tate, Deborah; Sherwood, Nancy; Jeffery, Robert; Wing, Rena

    2005-01-01

    In this study, the authors examined whether the number or success of weight loss partners influences participants' outcomes in behavioral weight loss treatment. Overweight participants (n=109) assigned to an exercise intensive group in a larger trial were encouraged to invite up to 3 partners to attend treatment. Weight losses at 6, 12, and 18…

  2. THE PHYSICAL HEALTH CONSEQUENCES OF INTIMATE PARTNER

    African Journals Online (AJOL)

    Therefore, the objective of this study was to assess the physical health consequences of intimate partner violence against women and the coping mechanisms in Agaro town, Southwest Ethiopia. METHODS: - This community based cross-sectional study was conducted among 510 ever-partnered women in. Agaro town from ...

  3. Partners in Science. [CD-ROM].

    Science.gov (United States)

    2000

    Partners in Science students, aided by community professionals, learn science by designing and conducting their own research. Partners in Science brings the community and schools closer together through a mentorship program. Scientists, in fields ranging from wildlife biology to space physics, are in frequent contact with classes and home-schooled…

  4. Intimate Partner Violence Victimization and Associated Factors ...

    African Journals Online (AJOL)

    AJRH Managing Editor

    Abstract. The study aimed at investigating the prevalence of intimate partner violence (IPV) and its associated factors among male and ... Keywords: partner violence, undergraduate students, sociodemographic factors, risk factors, protective factors, multi-country. Résumé ...... Masculinity 2007; 8(4): 225-239. 31. Gelaye B ...

  5. Increasing HIV testing among male partners

    NARCIS (Netherlands)

    Orne-Gliemann, Joanna; Balestre, Eric; Tchendjou, Patrice; Miric, Marija; Darak, Shrinivas; Butsashvili, Maia; Perez-Then, Eddy; Eboko, Fred; Plazy, Melanie; Kulkarni, Sanjeevani; du Lou, Annabel Desgrees; Dabis, Francois

    2013-01-01

    Objective: Couple-oriented posttest HIV counselling (COC) provides pregnant women with tools and strategies to invite her partner to HIV counselling and testing. We conducted a randomized trial of the efficacy of COC on partner HIV testing in low/medium HIV prevalence settings (Cameroon, Dominican

  6. Sex Differences in Attitudes toward Partner Infidelity

    Directory of Open Access Journals (Sweden)

    Michael J. Tagler

    2013-10-01

    Full Text Available Sex differences in reactions to partner infidelity have often been studied by comparing emotional reactions to scenarios of sexual versus emotional infidelity. Men, relative to women, tend to react with more distress to partner sexual infidelity than to emotional infidelity. Evolutionary theorists interpret this difference as evidence of sexually dimorphic selection pressures. In contrast, focusing only on the simple effects within each sex, social-cognitive theorists suggest that men and women do not differ in their reactions to partner infidelity. As evidenced by recent rival meta-analytic reports, these diverging perspectives remain largely unresolved and contentious. The present study was designed to take a new approach by measuring attitudes toward partner infidelity. Results were consistent with the evolutionary perspective: Men, to a significantly larger degree than women, evaluated partner sexual infidelity more negatively than emotional infidelity.

  7. Sex differences in attitudes toward partner infidelity.

    Science.gov (United States)

    Tagler, Michael J; Jeffers, Heather M

    2013-08-06

    Sex differences in reactions to partner infidelity have often been studied by comparing emotional reactions to scenarios of sexual versus emotional infidelity. Men, relative to women, tend to react with more distress to partner sexual infidelity than to emotional infidelity. Evolutionary theorists interpret this difference as evidence of sexually dimorphic selection pressures. In contrast, focusing only on the simple effects within each sex, social-cognitive theorists suggest that men and women do not differ in their reactions to partner infidelity. As evidenced by recent rival meta-analytic reports, these diverging perspectives remain largely unresolved and contentious. The present study was designed to take a new approach by measuring attitudes toward partner infidelity. Results were consistent with the evolutionary perspective: Men, to a significantly larger degree than women, evaluated partner sexual infidelity more negatively than emotional infidelity.

  8. Advice from working women with retired partners.

    Science.gov (United States)

    Cooley, Eileen L; Adorno, Gail

    2016-01-01

    in the 21st century, as more women are employed full-time and couples increasingly share egalitarian values, more women continue employment after their partners have voluntarily retired. However, we know very little about the experiences of this growing population of women. We asked working women with retired partners to share their advice for other women who may face this developmental transition. Open-ended responses from 97 women were analyzed to identify pertinent issues and themes. Four primary content areas were identified: time management, division of household labor, financial planning, and communication. Communication between partners was both a topic of concern as well as the solution suggested to resolve conflicts or differences that may arise when women live with a retired partner. It is expected that future changes in the workforce and improvements in the gender balance within relationships will continue to impact experiences for working women with retired partners.

  9. Analysis of the human HP1 interactome reveals novel binding partners

    DEFF Research Database (Denmark)

    Rosnoblet, Claire; Vandamme, Julien; Völkel, Pamela

    2011-01-01

    Heterochromatin protein 1 (HP1) has first been described in Drosophila as an essential component of constitutive heterochromatin required for stable epigenetic gene silencing. Less is known about the three mammalian HP1 isotypes CBX1, CBX3 and CBX5. Here, we applied a tandem affinity purification...... approach coupled with tandem mass spectrometry methodologies in order to identify interacting partners of the mammalian HP1 isotypes. Our analysis identified with high confidence about 30-40 proteins co-eluted with CBX1 and CBX3, and around 10 with CBX5 including a number of novel HP1-binding partners. Our...... data also suggest that HP1 family members are mainly associated with a single partner or within small protein complexes composed of limited numbers of components. Finally, we showed that slight binding preferences might exist between HP1 family members....

  10. Husband/Partner Intoxication and Intimate Partner Violence Against Women in the Philippines.

    Science.gov (United States)

    Kerridge, Bradley T; Tran, Phu

    2016-09-01

    This study examined husband/partner intoxication and experience with physical, sexual, and emotional intimate partner violence against women (IPVAW) using data derived from a nationally representative survey conducted in the Philippines in 2013. Multivariate logistic regression analyses were used to examine the association between intoxication and 3 different types of intimate partner violence against women. Multinomial logistic regression was used to examine intoxication and severity of violence. In this sample, 28.8% of women reported experiencing any form of intimate partner violence and 92.9% of women reported their partner being intoxicated at least sometimes. Intoxication was significantly associated with all 3 types of intimate partner violence, while the odds of experiencing one form of IPVAW versus no form of IPVAW and 2 forms of IPVAW versus 1 form of IPVAW was greater among women reporting frequency of husband/partner intoxication as often. © 2016 APJPH.

  11. Male partner selectivity, romantic confidence, and media depictions of partner scarcity.

    Science.gov (United States)

    Taylor, Laramie D

    2013-01-18

    An experiment was conducted to explore the effects of exposure to partner scarcity or abundance messages on men's partner selectivity, romantic confidence, and self-assessed attractiveness. Undergraduate male participants watched a soap opera narrative featuring either two men competing over one potential female partner (partner scarcity) or two women competing over one potential male partner (partner abundance). Relative to control subjects, watching either narrative reduced romantic confidence. Experimental condition also affected partner selectivity and self-assessed attractiveness, though both effects were moderated by endorsement of traditional masculine ideology. Viewing the abundance narrative resulted in greater selectivity and self-assessed attractiveness for men high in endorsement of traditional masculinity but diminished selectivity and self-assessed attractiveness for men low in endorsement of traditional masculine identity.

  12. Gastrin-induced proliferation involves MEK partner 1 (MP1)

    OpenAIRE

    Steigedal, Tonje S; Prestvik, Wenche S.; Selvik, Linn-Karina M.; Christina S Fjeldbo; Bruland, Torunn; L?greid, Astrid; Thommesen, Liv

    2013-01-01

    The peptide hormone gastrin is an important factor for the maintenance and homeostasis of the gastric mucosa. We show that gastrin stimulates proliferation in a dose-dependent manner in the human gastric adenocarcinoma cell line AGS-GR. Furthermore, we demonstrate that the MAPK scaffold protein MEK partner 1 (MP1) is important for gastrin-induced phosphorylation of ERK1 and ERK2 and that MP1 promotes gastrin-induced proliferation of AGS-GR cells. Our results suggest a role of MP1 in gastrin-i...

  13. Pesticide Advisory Committees and Regulatory Partners

    Science.gov (United States)

    This site will provide all stakeholders, including the general public, with access to information about meetings of advisory committees, and how we work with state, territory, and tribal government partners.

  14. Partnering with the PESP Landscaping Initiative

    Science.gov (United States)

    The Landscaping Initiative works with its partners to educate do-it-yourself homeowners, lawn care customers, retailers and consumers at point-of-sale, and schools and school districts about pest management alternatives and proper pesticide use.

  15. Climate Ready Estuaries Partner Projects Map

    Science.gov (United States)

    CRE partners with the National Estuary Program to develop climate change projects in coastal U.S. areas, such as bays and harbors; to develop adaptation action plans, identify climate impacts and indicators, and more. This map shows project locations.

  16. Gender symmetry, sexism, and intimate partner violence.

    Science.gov (United States)

    Allen, Christopher T; Swan, Suzanne C; Raghavan, Chitra

    2009-11-01

    This study of a predominantly Hispanic sample of 92 male and 140 female college students examines both gender symmetry in intimate partner violence (IPV) and inconsistent relationships found in previous studies between sexist attitudes and IPV. Results indicate that although comparable numbers of men and women perpetrate and are victimized in their relationships with intimate partners, the path models suggest that women's violence tends to be in reaction to male violence, whereas men tend to initiate violence and then their partners respond with violence. Benevolent sexism was shown to have a protective effect against men's violence toward partners. Findings highlight the importance of studying women's violence not only in the context of men's violence but also within a broader sociocultural context.

  17. Featured Partner: Saddle Creek Logistics Services

    Science.gov (United States)

    This EPA fact sheet spotlights Saddle Creek Logistics as a SmartWay partner committed to sustainability in reducing greenhouse gas emissions and air pollution caused by freight transportation, partly by growing its compressed natural gas (CNG) vehicles for

  18. Social partners and gender equality in Europe

    OpenAIRE

    Tilly, Pierre; Weber, Tina

    2014-01-01

    The growing participation of women in the labour market has prompted changes in the way European social partner organisations tackle gender issues. Their organisational and collective bargaining structures – historically dominated by men – have had to adapt to include gender equality issues. This report examines the extent to which gender equality is incorporated by the social partners in their organisations and in their policymaking at European and national level. It explores the differences...

  19. Do aphids actively search for ant partners?

    OpenAIRE

    Fischer, Christophe; Vanderplanck, Maryse; Lognay, Georges; Detrain, Claire; Verheggen, François

    2015-01-01

    The aphid–ant mutualistic relationships are not necessarily obligate for neither partners but evidence is that such interactions provide them strong advantages in terms of global fitness. While it is largely assumed that ants actively search for their mutualistic partners namely using volatile cues; whether winged aphids (i.e. aphids’ most mobile form) are able to select ant-frequented areas had not been investigated so far. Ant-frequented sites would indeed offer several advantages for these...

  20. Partner Caregiving in Older Cohabiting Couples

    Science.gov (United States)

    2011-01-01

    Objectives. Despite the rapidly increasing prevalence of cohabitation among older adults, the caregiving literature has exclusively focused on formally married individuals. Extending prior work on intra-couple care, this study contrasts frail cohabitors’ patterns of care receipt from a partner to that of frail spouses. Methods. Using nationally representative panel data from the Health and Retirement Study (2000, 2002, 2004, and 2006), we estimate random effects cross-sectional times series models predicting frail cohabitors’ likelihood of receiving partner care compared with their married counterparts’. Conditional on the receipt of intra-couple care, we also examine differences in marital and nonmarital partners’ caregiving hours and caregiving involvement relative to other helpers. Results. Net of sociodemographic, disability, and comorbidity factors, we find that cohabitors are less likely to receive partner care than married individuals. However, caregiving nonmarital partners provide as many hours of care as spouses while providing a substantially larger share of disabled respondents’ care than marital partners. Discussion. Cohabitation and marriage have distinct implications for older adults’ patterns of partner care receipt. This study adds weight to a growing body of research emphasizing the importance of accounting for older adults’ nontraditional union forms and of examining the ramifications of cohabitation for older adults’ well-being. PMID:21482588

  1. Partner choice creates fairness in humans.

    Science.gov (United States)

    Debove, Stéphane; André, Jean-Baptiste; Baumard, Nicolas

    2015-06-07

    Many studies demonstrate that partner choice has played an important role in the evolution of human cooperation, but little work has tested its impact on the evolution of human fairness. In experiments involving divisions of money, people become either over-generous or over-selfish when they are in competition to be chosen as cooperative partners. Hence, it is difficult to see how partner choice could result in the evolution of fair, equal divisions. Here, we show that this puzzle can be solved if we consider the outside options on which partner choice operates. We conduct a behavioural experiment, run agent-based simulations and analyse a game-theoretic model to understand how outside options affect partner choice and fairness. All support the conclusion that partner choice leads to fairness only when individuals have equal outside options. We discuss how this condition has been met in our evolutionary history, and the implications of these findings for our understanding of other aspects of fairness less specific than preferences for equal divisions of resources. © 2015 The Author(s) Published by the Royal Society. All rights reserved.

  2. Working models of attachment to parents and partners: implications for emotional behavior between partners.

    Science.gov (United States)

    Mehta, Neera; Cowan, Philip A; Cowan, Carolyn P

    2009-12-01

    This study examined whether working models of attachment are associated with observed positive emotion, sadness, and anger during marital conflict. Individuals (n = 176) from a longitudinal study of families participated in the current cross-sectional study. Narrative interviews assessed the unique and combined contribution of attachment representations based on parents (adult attachment) and partner (couple attachment). The influence of partner's attachment, depression symptoms, and sex of participant was also examined. Hierarchical linear models demonstrated that one's couple attachment security predicts one's observed positive emotion, whereas the partner's couple attachment security predicts one's observed negative emotion. Partner's depression symptoms moderated the effects of partner's couple attachment. Adult attachment was not related to observed emotional behavior between partners. These findings have important clinical implications for individual, couple, and family therapy.

  3. Intimate partner violence and maternal educational practice

    Directory of Open Access Journals (Sweden)

    Josianne Maria Mattos da Silva

    Full Text Available ABSTRACT OBJECTIVE The objective of this study is to analyze the association between intimate partner violence against women and maternal educational practice directed to children at the beginning of formal education. METHODS This is a cross-sectional study, carried out between 2013 and 2014, with 631 mother/child pairs, registered in the Family Health Strategy of the Health District II of the city of Recife, State of Pernambuco, Brazil. It integrates a prospective cohort study designed to investigate the consequences of exposure to intimate partner violence in relation to the child who was born between 2005 and 2006. The maternal educational practice has been assessed by the Parent-Child Conflict Tactics Scale and the intimate partner violence by a questionnaire adapted from the Multi-Country Study on Women’s Health and Domestic Violence of the World Health Organization. Intimate partner violence referred to the last 12 months and was defined by specific acts of psychological, physical, and sexual violence inflicted to women by the partner. The crude and adjusted prevalence ratios were estimated for the association studied, using log-binomial regression. RESULTS The prevalence of intimate partner violence was 24.4%, and violent maternal educational practice was 93.8%. The use of non-violent discipline was mentioned by 97.6% of the women, coexisting with violent strategies of discipline. Children whose mothers reported intimate partner violence presented a higher chance of suffering psychological aggression (PR = 2.2; 95%CI 1.0–4.7. CONCLUSIONS The violence suffered by the mother interferes in the parental education. The findings show high prevalence of violent maternal educational practice, pointing to the need for interventions that minimize the damage of violence in women and children.

  4. Intimate partner violence and maternal educational practice

    Science.gov (United States)

    da Silva, Josianne Maria Mattos; Lima, Marília de Carvalho; Ludermir, Ana Bernarda

    2017-01-01

    ABSTRACT OBJECTIVE The objective of this study is to analyze the association between intimate partner violence against women and maternal educational practice directed to children at the beginning of formal education. METHODS This is a cross-sectional study, carried out between 2013 and 2014, with 631 mother/child pairs, registered in the Family Health Strategy of the Health District II of the city of Recife, State of Pernambuco, Brazil. It integrates a prospective cohort study designed to investigate the consequences of exposure to intimate partner violence in relation to the child who was born between 2005 and 2006. The maternal educational practice has been assessed by the Parent-Child Conflict Tactics Scale and the intimate partner violence by a questionnaire adapted from the Multi-Country Study on Women’s Health and Domestic Violence of the World Health Organization. Intimate partner violence referred to the last 12 months and was defined by specific acts of psychological, physical, and sexual violence inflicted to women by the partner. The crude and adjusted prevalence ratios were estimated for the association studied, using log-binomial regression. RESULTS The prevalence of intimate partner violence was 24.4%, and violent maternal educational practice was 93.8%. The use of non-violent discipline was mentioned by 97.6% of the women, coexisting with violent strategies of discipline. Children whose mothers reported intimate partner violence presented a higher chance of suffering psychological aggression (PR = 2.2; 95%CI 1.0–4.7). CONCLUSIONS The violence suffered by the mother interferes in the parental education. The findings show high prevalence of violent maternal educational practice, pointing to the need for interventions that minimize the damage of violence in women and children. PMID:28423138

  5. Increasing HIV testing among male partners.

    Science.gov (United States)

    Orne-Gliemann, Joanna; Balestre, Eric; Tchendjou, Patrice; Miric, Marija; Darak, Shrinivas; Butsashvili, Maia; Perez-Then, Eddy; Eboko, Fred; Plazy, Melanie; Kulkarni, Sanjeevani; Desgrées du Loû, Annabel; Dabis, François

    2013-04-24

    Couple-oriented posttest HIV counselling (COC) provides pregnant women with tools and strategies to invite her partner to HIV counselling and testing. We conducted a randomized trial of the efficacy of COC on partner HIV testing in low/medium HIV prevalence settings (Cameroon, Dominican Republic, Georgia, India). Pregnant women were randomized to receive standard posttest HIV counselling or COC and followed until 6 months postpartum. Partner HIV testing events were notified by site laboratories, self-reported by women or both combined. Impact of COC on partner HIV testing was measured in intention-to-treat analysis. Socio-behavioural factors associated with partner HIV testing were evaluated using multivariable logistic regression. Among 1943 pregnant women enrolled, partner HIV testing rates (combined indicator) were 24.7% among women from COC group versus 14.3% in standard posttest HIV counselling group in Cameroon [odds ratio (OR) = 2.0 95% CI (1.2-3.1)], 23.1 versus 20.3% in Dominican Republic [OR = 1.2 (0.8-1.8)], 26.8 versus 1.2% in Georgia [OR = 29.6 (9.1-95.6)] and 35.4 versus 26.6% in India [OR = 1.5 (1.0-2.2)]. Women having received COC did not report more conjugal violence or union break-ups than in the standard posttest HIV counselling group. The main factors associated with partner HIV testing were a history of HIV testing among men in Cameroon, Dominican Republic and Georgia and the existence of couple communication around HIV testing in Georgia and India. A simple prenatal intervention taking into account the couple relationship increases the uptake of HIV testing among men in different socio-cultural settings. COC could contribute to the efforts towards eliminating mother-to-child transmission of HIV.

  6. Partner Market Opportunities and Relationship Stability

    Directory of Open Access Journals (Sweden)

    Ingmar Rapp

    2015-10-01

    Full Text Available Although partner market opportunities are generally considered to be important for relationship stability, they have never been measured accurately. In order to be able to test the anticipated effects of partner market opportunities, this study conceptualises them as individual opportunities for contact and interaction in concrete social contexts, like the neighbourhood, the workplace, leisure activities, etc. Using data from the German Marriage Market Survey, we first examine the impact of individual partner market opportunities on the risk of separation. Second, we examine to what extend the most frequently studied determinants of divorce and separation depend on partner market opportunities. Our results show that the number of opposite sex contacts increases the probability of separation. Sharing the same contacts with one’s partner decreases the risk of separation. Our results indicate further that reducing opposite sex contacts in the course of the relationship is partly responsible for the higher stability of longer-lasting relationships. Having a migration background is associated with fewer opposite sex contacts. This means that having a migration background would be more destabilising if these individuals did not have less opposite sex contacts than individuals without a migration background. In contrast, joint home ownership, church attendance, higher education and residing in western Germany would generally be more stabilising if these factors were not connected with more opposite sex contacts.

  7. Partner preferences among survivors of betrayal trauma.

    Science.gov (United States)

    Gobin, Robyn L

    2012-01-01

    Betrayal trauma theory suggests that social and cognitive development may be affected by early trauma such that individuals develop survival strategies, particularly dissociation and lack of betrayal awareness, that may place them at risk for further victimization. Several experiences of victimization in the context of relationships predicated on trust and dependence may contribute to the development of relational schema whereby abuse is perceived as normal. The current exploratory study investigates interpersonal trauma as an early experience that might impact the traits that are desired in potential romantic partners. Participants in the current study were asked to rate the desirability of several characteristics in potential romantic partners. Although loyalty was desirable to most participants regardless of their trauma history, those who reported experiences of high betrayal trauma rated loyalty less desirable than those who reported experiences of traumas that were low and medium in betrayal. Participants who reported experiences of revictimization (defined as the experience of trauma perpetrated by a close other during 2 different developmental periods) differed from participants who only reported 1 experience of high betrayal trauma in their self-reported desire for a romantic partner who possessed the traits of sincerity and trustworthiness. Preference for a partner who uses the tactic of verbal aggression was also associated with revictimization status. These preliminary findings suggest that victimization perpetrated by close others may affect partner preferences.

  8. Partner notification and partner treatment for chlamydia: attitude and practice of general practitioners in the Netherlands.

    NARCIS (Netherlands)

    Broek, I. van den; Donker, G.A.; Hek, K.; Bergen, J. van; Götz, H.

    2017-01-01

    Introduction: Chlamydia prevalence remains high despite scaled-up control efforts. In the Netherlands, the majority of chlamydia patients are seen by general practitioners (GPs). Partner notification (PN) and partner treatment (PT) are addressed in GP guidelines but may not be fully covered in daily

  9. A Community–Academic Partnered Grant Writing Series to Build Infrastructure for Partnered Research

    Science.gov (United States)

    Pardo, Yvette‐Janine; Norris, Keith C.; Diaz‐Romero, Maria; Morris, D'Ann; Vassar, Stefanie D.; Brown, Arleen F.

    2015-01-01

    Abstract Grant writing is an essential skill necessary to secure financial support for community programs and research projects. Increasingly, funding opportunities for translational biomedical research require studies to engage community partners, patients, or other stakeholders in the research process to address their concerns. However, there is little evidence on strategies to prepare teams of academic and community partners to collaborate on grants. This paper presents the description and formative evaluation of a two‐part community–academic partnered grant writing series designed to help community organizations and academic institutions build infrastructure for collaborative research projects using a partnered approach. The first phase of the series was a half‐day workshop on grant readiness, which was open to all interested community partners. The second phase, open only to community–academic teams that met eligibility criteria, was a 12‐week session that covered partnered grant writing for foundation grants and National Institutes of Health grants. Participants in both phases reported an increase in knowledge and self‐efficacy for writing partnered proposals. At 1‐year follow‐up, participants in Phase 2 had secured approximately $1.87 million in funding. This community–academic partnered grant writing series helped participants obtain proposal development skills and helped community–academic teams successfully compete for funding. PMID:26365589

  10. A Community-Academic Partnered Grant Writing Series to Build Infrastructure for Partnered Research.

    Science.gov (United States)

    King, Keyonna M; Pardo, Yvette-Janine; Norris, Keith C; Diaz-Romero, Maria; Morris, D'Ann; Vassar, Stefanie D; Brown, Arleen F

    2015-10-01

    Grant writing is an essential skill necessary to secure financial support for community programs and research projects. Increasingly, funding opportunities for translational biomedical research require studies to engage community partners, patients, or other stakeholders in the research process to address their concerns. However, there is little evidence on strategies to prepare teams of academic and community partners to collaborate on grants. This paper presents the description and formative evaluation of a two-part community-academic partnered grant writing series designed to help community organizations and academic institutions build infrastructure for collaborative research projects using a partnered approach. The first phase of the series was a half-day workshop on grant readiness, which was open to all interested community partners. The second phase, open only to community-academic teams that met eligibility criteria, was a 12-week session that covered partnered grant writing for foundation grants and National Institutes of Health grants. Participants in both phases reported an increase in knowledge and self-efficacy for writing partnered proposals. At 1-year follow-up, participants in Phase 2 had secured approximately $1.87 million in funding. This community-academic partnered grant writing series helped participants obtain proposal development skills and helped community-academic teams successfully compete for funding. © 2015 Wiley Periodicals, Inc.

  11. A review of partner notification for sex partners of men infected with Chlamydia.

    Science.gov (United States)

    Hogben, Matthew; Kissinger, Patricia

    2008-11-01

    A discussion of the feasibility and use of chlamydial screening of men requires attention to management of their partners. Because of the large numbers of chlamydial cases in the United States, public health-mediated partner notification, as a first line partner management strategy, is not practical. This article reviews the evidence for patient-based referral. We reviewed studies (1997-2007) from the United States and other industrialized nations in which men diagnosed with chlamydia were exposed to some form of partner referral instruction. Randomized controlled trial and observational data were included; where data permitted, we estimated proportions of partners notified and treated. Nine studies from 3 countries yielded 8 estimates of notification rates and 10 of treatment rates. Estimates varied according to whether patient referral was accompanied by counseling, contact slips, or medications for partners. Overall, 48% to 79% of partners seemed to be notified with a smaller proportion subsequently treated (30%-61%). Higher rates of notification and treatment were associated with various enhancements to basic referral instructions, especially if patients were offered medications to bring to partners. Data also suggest a role for contact slips. Resource constraints suggest that public health investigation should be limited to high-priority cases (e.g., where evidence of dense sexual networks exists) and monitoring of patient referral efforts.

  12. Partner Killing by Men in Cohabiting and Marital Relationships

    Science.gov (United States)

    Shackelford, Todd K.; Mouzos, Jenny

    2005-01-01

    Using a national-level U.S. database, T. K. Shackelford (2001) calculated rates of uxoricide (the murder of a woman by her romantic partner) by relationship type (cohabiting or marital), by ages of the partners, and by the age difference between partners. Women in cohabiting relationships were 9 times more likely to be killed by their partner than…

  13. Self-Efficacy Mediates the Association between Partner Trust and ...

    African Journals Online (AJOL)

    AJRH Managing Editor

    psychological resilience and partner trust to increase understanding of trust towards one's intercourse partner. Mediation analyses, stratified by gender, reveal that condom usage is predicted by self-efficacy and partner trust among females but not males. Higher psychological resilience predicts lower partner trust among ...

  14. The severity of violence against women by intimate partners and ...

    African Journals Online (AJOL)

    Having a partner with problem drinking and drug use (OR = 2.80, CI = 1.35–5.79) was associated with an increased psychological intimate partner abuse. Problem drinking and drug use among male partners is a strong determinant of physical intimate partner violence among battered women in South Africa. Intimate ...

  15. RISK FACTORS FOR INTIMATE PARTNER VIOLENCE

    Directory of Open Access Journals (Sweden)

    Ceren Atakay

    2014-06-01

    Full Text Available Intimate partner violence has kept being one of the major societal issues in our country over the past year. It is absolutely necessary to intervene in this substantially psychological issue multi-directionally. In order to intervene in the problem from psychological aspect, it is important to estimate and interpret the risk factors for intimate partner violence. Therefore in the current study, ‘I-cube theory’ which is about the risk factors for intimate partner violence has been explained first. Afterwards, the findings of content analysis which was obtained from newspaper reports about femicide in 2013 have been shown and these findings have been discussed within the context of I-cube theory, respectively. Finally, solutions to prevent this violence has been suggested.

  16. Welcoming spouses and partners to CERN

    CERN Multimedia

    Staff Association

    2016-01-01

    The questions related to Diversity were examined in the framework of the Five-yearly Review, which was approved in December 2015. The first themes implemented this year concern helping spouses or partners integrate into the working world, and improving the balance between professional and private life.   To this end, the Social Affairs Service and the CERN Diversity Office organised together on Tuesday, 15 November, a “Welcome Drink” for the spouses and partners of employed members of the personnel of CERN who have recently arrived in the region. This event was an occasion for the spouses and partners to meet and greet with various internal services at CERN, including the Staff Association, as well as local networks that can provide assistance in integrating into the region, in terms of job search for instance. Therefore, several service providers were present, including: the Geneva Welcome Center (CAGI), a welcoming network for newly arrived employees of international organi...

  17. Sleep and partner-specific quality of life in partners of men with lower urinary tract symptoms compared with partners of men from the general population.

    Science.gov (United States)

    Marklund, Helén; Spångberg, Anders; Edéll-Gustafsson, Ulla

    2015-01-01

    The aims of this study were to examine differences between partners of men with lower urinary tract symptoms (LUTS) suggestive of benign prostatic obstruction (BPO) and partners of men from the population regarding sleep and two aspects of quality of life, partner-specific quality of life and health-related quality of life (HRQoL), and to identify factors related to the partner-specific quality of life and the parameter sleep efficiency. The design was descriptive and comparative. The subjects were partners of men with LUTS suggestive of BPO (n = 126) and partners of randomly selected men from the general population (n = 131). Self-administered questionnaires about demography, comorbidity, sleep, sexuality, partner-specific quality of life and HRQoL were used. Partners of men with LUTS suggestive of BPO were significantly more affected in all variables measuring partner-specific quality of life compared with partners from the population. The most impaired aspects were compassion and worry about an operation or cancer. In logistic regression, the only explanatory factors were having a partner belonging to the LUTS group for impaired partner-specific quality of life and having a bed partner for high sleep efficiency. There were no significant differences between the two groups regarding the quantity and quality of sleep or the HRQoL. The partner-specific quality of life was impaired in partners of men with LUTS suggestive of BPO. Sleep and HRQoL did not differ between partners of men with LUTS and partners from the population.

  18. Whom to Choose as License Partner?

    DEFF Research Database (Denmark)

    Laursen, Keld; Reichstein, Toke; Trombini, Giulia

    2013-01-01

    This paper investigates the matching of firms on the market for technology. The paper forwards two dimensions along which license formation occurs: technology and product-market. Both sides of the market search for a partner representing potential for high technology synergies to maximize licensing...... between technology and market forces: if partners are market distant, the likelihood of technology license contractual partnership decreases with partners’ technological distance. Using data on the formation of license partnerships in the global biopharmaceutical industry over the period 1994...

  19. Partnering i udførelse

    DEFF Research Database (Denmark)

    Gottlieb, Stefan; Bang, H.L.

    Publikationen sammenfatter erfaringerne vedrørende brug af partnering i udførelsesfasen af LO's hovedsæde i Havnestaden, København. Forløbet af udførelsesfasen viser, at der er betydelige barrierer for at omstille sig til samarbejde efter en partneringsmodel, men også at samarbejdet løbende er...... udførelse. Projektets parter er alligevel positivt indstillet til samarbejde i partnering, fordi væsentlige succeskriterier, herunder kvaliteten af det afleverede byggeri og friheden til at blive klogere undervejs, er opfyldt....

  20. Pharma partnering: other people’s science

    Science.gov (United States)

    Hofmann, Christian

    2017-01-01

    Partnering is an ideal field if someone is seeking to move from a scientific to a more business-oriented discipline. Partnering’s goal is to identify and acquire external innovation. These discoveries are then included in a company’s pipeline and help bring novel treatments to patients. Advanced scientific training is essential in the identification and evaluation of these external assets. Here I describe how partnering works in a pharmaceutical company and offer advice on how to make a successful transition from a PhD program to a business career. PMID:28035041

  1. Sexuality and the Commission of Physical Violence to Partners and Non-Partners by Men and Women

    Science.gov (United States)

    Cogan, Rosemary; Fennell, Tiffanie

    2007-01-01

    In 2 studies of physical violence and sexuality among college students, more than 75% of men and more than 60% of women reported committing physical violence in the past year, including more women to partners and more men to non-partners. More than 90% of men who committed violence to partners were also violent to non-partners. In Study 1, among…

  2. Immunoprecipitation-based analysis of protein-protein interactions.

    Science.gov (United States)

    Speth, Corinna; Toledo-Filho, Luis A A; Laubinger, Sascha

    2014-01-01

    Several techniques allow the detection of protein-protein interactions. In vivo co-immunoprecipitation (Co-IP) studies are an important complement to other commonly used techniques such as yeast two-hybrid or fluorescence complementation, as they reveal interactions between functional proteins at physiological relevant concentrations. Here, we describe an in vivo Co-IP approach using either GFP affinity matrix or specific antibodies to purify proteins of interests and their interacting partners.

  3. Male partner reproductive coercion among women veterans.

    Science.gov (United States)

    Rosenfeld, Elian A; Miller, Elizabeth; Zhao, Xinhua; Sileanu, Florentina E; Mor, Maria K; Borrero, Sonya

    2017-10-19

    Male partner reproductive coercion is defined as male partners' attempts to promote pregnancy through interference with women's contraceptive behaviors and reproductive decision-making. Male partners may try to promote pregnancy through birth control sabotage such as taking away or destroying their partners' contraceptives, refusing to wear condoms, and/or verbally pressuring their partners to abstain from contraceptive use. Reproductive coercion is associated with an elevated risk for unintended pregnancy. Women who experience intimate partner violence, who are in racial/ethnic minorities, and who are of lower socioeconomic status are more likely to experience reproductive coercion. Women veterans who use Veterans Affairs for health care may be particularly vulnerable to reproductive coercion because they are disproportionally from racial/ethnic minority groups and experience high rates of intimate partner violence. We sought to examine the prevalence, correlates, and impact of reproductive coercion among women veterans who are served by the Veterans Affairs healthcare system. We analyzed data from a national telephone survey of women veterans aged 18-44 years, with no history of sterilization or hysterectomy, who had received care within the Veterans Affairs system in the previous 12 months. Participants who had sex with men in the last year were asked if they experienced male partner reproductive coercion. Adjusted logistic regression was used to examine the relationship between participant characteristics and male partner reproductive coercion and the relationship between reproductive coercion and the outcomes of contraceptive method used at last sex and pregnancy and unintended pregnancy in the last year. Among the 1241 women veterans in our study cohort, 11% reported experiencing male partner reproductive coercion in the past year. Black women, younger women, and single women were more likely to report reproductive coercion than their white, older, and

  4. Ecology and economics: Partners in theological conversation

    African Journals Online (AJOL)

    Book Review http://www.hts.org.za doi:10.4102/hts.v67i3.1133. Ecology and economics: Partners in theological conversation. Book Title: Theology that matters: Ecology, economy, and God. Book Cover: Editor: Darby K. Ray. ISBN: 9780800637941. Publisher: Fortress, Minneapolis, p. 245, $13.20*. *Book price at time of ...

  5. Strategy Guideline: Partnering for High Performance Homes

    Energy Technology Data Exchange (ETDEWEB)

    Prahl, D.

    2013-01-01

    High performance houses require a high degree of coordination and have significant interdependencies between various systems in order to perform properly, meet customer expectations, and minimize risks for the builder. Responsibility for the key performance attributes is shared across the project team and can be well coordinated through advanced partnering strategies. For high performance homes, traditional partnerships need to be matured to the next level and be expanded to all members of the project team including trades, suppliers, manufacturers, HERS raters, designers, architects, and building officials as appropriate. In an environment where the builder is the only source of communication between trades and consultants and where relationships are, in general, adversarial as opposed to cooperative, the chances of any one building system to fail are greater. Furthermore, it is much harder for the builder to identify and capitalize on synergistic opportunities. Partnering can help bridge the cross-functional aspects of the systems approach and achieve performance-based criteria. Critical success factors for partnering include support from top management, mutual trust, effective and open communication, effective coordination around common goals, team building, appropriate use of an outside facilitator, a partnering charter progress toward common goals, an effective problem-solving process, long-term commitment, continuous improvement, and a positive experience for all involved.

  6. PARTNER Project 8: Sonic boom mitigation

    Science.gov (United States)

    Sparrow, Victor W.

    2005-09-01

    Current U.S. and international laws prohibit commercial supersonic flight over land due to the impact of conventional sonic boom noise. Aircraft manufacturers, however, now have modern computational fluid dynamics and optimization tools, unavailable when those laws were enacted, that will allow them to design and build aircraft with boom signatures that are substantially smoothed compared with traditional N-waves. One purpose of the FAA/NASA/Transport Canada PARTNER Center of Excellence Project 8 is to determine exactly which waveforms would be heard by the public if low-boom supersonic aircraft are put into service. Another purpose is to ascertain the acceptability of those waveforms. The project involves the following universities, government, and industry partners: Penn State, Purdue, Stanford, the National Aeronautics and Space Administration, the Federal Aviation Administration, Boeing, Cessna, Gulfstream, Lockheed-Martin, and Wyle Laboratories. Some of the initial project work includes studies on the propagation of sonic booms through atmospheric turbulence, on the mutual reproducibility of three sonic boom simulators, and on the realism of those simulators as determined by expert listeners. The results of all the studies are intended to provide the FAA with new data to reassess current regulations. [Work supported by NASA, the FAA, and the PARTNER industrial partners.

  7. Mental health, intimate partner violence and HIV

    African Journals Online (AJOL)

    Mental health, intimate partner violence and HIV. N Woollett,1 MA (Psychology, Art Therapy); A M Hatcher,1,2 MPhil (Sociology). 1 Wits Reproductive Health and HIV Institute, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa. 2 School of Public Health, Faculty of Health Sciences, ...

  8. Partner Selection for People with Intellectual Disabilities.

    Science.gov (United States)

    Bates, Claire; Terry, Louise; Popple, Keith

    2017-07-01

    The aim of this research was to understand the characteristics that adults with intellectual disabilities look for in a partner. There have been numerous studies that have explored partner selection for people without intellectual disabilities, but no research that specifically identified the traits valued in a partner by people with intellectual disabilities. In-depth interviews were conducted with eleven participants across two UK sites. All participants were adults with an intellectual disability who had been in a relationship with a partner for over a year. The narratives were analysed utilizing hermeneutic phenomenology, guided by the theory of Van Manen (1990). The findings highlighted that, regardless of age, participant's relationships typically developed within a segregated environment for people with intellectual disabilities over the past 10 years. People with intellectual disabilities expressed a wish to be loved, to be treated kindly and to have companionship. However, they did not place high value on attributes such as financial security, social status or intelligence. The research demonstrated how poorly integrated people with intellectual disabilities are within mainstream society. Desired characteristics and expectations for participant's relationships were rooted in a shared history and culture, which was shaped by their intellectual disability and support needs. © 2016 John Wiley & Sons Ltd.

  9. Correcting abnormal speaking through communication partners ...

    African Journals Online (AJOL)

    The listed characteristics are called speech disorders. Abnormal speaking attracts some penalties to the speaker. The penalties are usually very disturbing to the speaker that undertaking some therapeutic measures becomes inevitable. Communication partners strategy is a speech correction approach which makes use of ...

  10. 77 FR 76380 - Partner's Distributive Share

    Science.gov (United States)

    2012-12-28

    ... Part 1 [TD 9607] RIN 1545-BJ37 Partner's Distributive Share AGENCY: Internal Revenue Service (IRS... partnerships that qualify, for the current tax year and all prior tax years, as pro rata partnerships, de... considered a pro rata partnership if all contributions to the partnership are cash; all items of partnership...

  11. Restricting mutualistic partners to enforce trade reliance

    NARCIS (Netherlands)

    Wyatt, G.A.K.; Kiers, E.T.; Gardner, A.

    2016-01-01

    Mutualisms are cooperative interactions between members of different species, often involving the trade of resources. Here, we suggest that otherwise-cooperative mutualists might be able to gain a benefit from actively restricting their partners' ability to obtain resources directly, hampering the

  12. Yorkie and Scalloped: partners in growth activation.

    Science.gov (United States)

    Bandura, Jennifer L; Edgar, Bruce A

    2008-03-01

    The Hippo (Hpo) signaling pathway limits organ growth in organisms from Drosophila to mammals by suppressing the activity of the transcriptional coactivator Yorkie (Yki)/YAP. The TEAD/TEF factor Scalloped (Sd) has been identified as the first known transcription factor to partner with Yki as a downstream target of Hpo signaling.

  13. partnering, poverty reduction and rural enterprise advancement

    African Journals Online (AJOL)

    p2333147

    Keywords: Agribusiness, extension, partnering, poverty alleviation, rural .... global competition. A major limitation, however, is its inability to respond to the needs of small-scale farmers, the majority of land users in the Eastern Cape Province. ii) ... but the changes in the complexities of the decision making environment.

  14. Partners and initiatives | IDRC - International Development ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    Results 1 - 10 of 18 ... There are no borders when it comes to issues like climate change, infectious disease, poverty, and instability. In today's world, the challenges facing developing countries affect us all. Meeting these challenges calls for a global response that mobilizes research partners everywhere. When we pool our ...

  15. Client acceptance decisions of Dutch auditing partners

    NARCIS (Netherlands)

    Wittek, Rafael; Zee, Theo van der; Mühlau, Peter

    2008-01-01

    A vignette study of 567 client acceptance decisions by 67 Dutch auditors showed that the prospect of acquiring additional assignments significantly increases the likelihood that auditing partners accept an audit assignment from a new client, thereby violating a rule of the auditing profession. Audit

  16. Coordinating the United States Interagency Partnering Effort

    Science.gov (United States)

    2013-03-01

    operations now will mean throwing 18 away hard-fought gains, and expose the United States to new risks from across the globalising ...analyzed, risks can be mitigated as they occur. Partner activities can and will have a significant impact on national security, and the application of

  17. Physical Health Effects of Intimate Partner Abuse

    Science.gov (United States)

    Sillito, Carrie LeFevre

    2012-01-01

    Although intimate partner violence has been recognized as both a social problem and health issue, the extent to which it is a health issue for both males and females in the general population is largely unknown. This longitudinal research uses data from the National Survey of Family and Households (1987-2003). Random effects logistic regression…

  18. Self and partner personality in intimate relationships

    NARCIS (Netherlands)

    Barelds, D.P.H.

    Two studies were conducted to examine the relations between both partners' personality and marital quality in married or cohabiting heterosexual couples. In Study 1 (N = 1380, or 690 couples), personality was assessed by means of the Dutch Personality Questionnaire, whereas in Study 2 (N = 564, or

  19. Intimate partner violence in orthopaedic trauma patients

    NARCIS (Netherlands)

    Sprague, S.A.

    2013-01-01

    Intimate partner violence (IPV) or domestic violence is a common and serious public health problem around the globe. Victims of IPV frequently present to health care practitioners including orthopaedic surgeons. Substantial research has been conducted on IPV over the past few decades, but very

  20. Monotop signature from a fermionic top partner

    Science.gov (United States)

    Gonçalves, Dorival; Kong, Kyoungchul; Sakurai, Kazuki; Takeuchi, Michihisa

    2018-01-01

    We investigate monotop signatures arising from phenomenological models of fermionic top partners, which are degenerate in mass and decay into a bosonic dark matter candidate, either spin 0 or spin 1. Such a model provides a monotop signature as a smoking gun, while conventional searches with t t ¯ + missing transverse momentum are limited. Two such scenarios, (i) a phenomenological third generation extradimensional model with excited top and electroweak sectors, and (ii) a model where only a top partner and a dark matter particle are added to the standard model, are studied in the degenerate mass regime. We find that in the case of extra dimension a number of different processes give rise to effectively the same monotop final state, and a great gain can be obtained in the sensitivity for this channel. We show that the monotop search can explore top-partner masses up to 630 and 300 GeV for the third generation extradimensional model and the minimal fermionic top-partner model, respectively, at the high luminosity LHC.

  1. Prevalence & Correlates of Intimate Partner physical violence ...

    African Journals Online (AJOL)

    Witnessing family violence as a girl child, education, place of residence, parity, duration of marriage ... also known as domestic violence, is the most endemic form ... different studies, the major risk factors of intimate partner violence include witnessing family violence as a child, young age, poverty, low social status, women's.

  2. Sleep Loss and Partner Violence Victimization

    Science.gov (United States)

    Walker, Robert; Shannon, Lisa; Logan, T. K.

    2011-01-01

    Intimate partner violence victimization has been associated with serious health problems among women, including many disorders that involve sleep disturbances. However, there has been only limited examination of sleep duration among women with victimization experiences. A total of 756 women with a domestic violence order (DVO) against a male…

  3. Intimate Partner Violence Victimization and Associated Factors ...

    African Journals Online (AJOL)

    In multivariate logistic regression analyses, among both men and women, sociodemographic factors (senior study year, living in a low or lower middle income country) and risk factors (history of childhood physical and sexual abuse, made someone pregnant or had been pregnant, having had two or more sexual partners in ...

  4. Intimate Partner Violence within Law Enforcement Families

    Science.gov (United States)

    Anderson, Anita S.; Lo, Celia C.

    2011-01-01

    Using data from the Baltimore Police Stress and Domestic Violence study, the authors examined how exposure to stressful events on the job affects law enforcement employees' physical aggression toward domestic partners, evaluating the role of negative emotions and authoritarian spillover in mediating the impact of such task-related stress. The…

  5. Alcohol Misuse and Multiple Sexual Partners

    Directory of Open Access Journals (Sweden)

    Shahrzad Bazargan-Hejazi, PhD

    2012-05-01

    Full Text Available Introduction: We examine the association between self-reported alcohol misuse and alcohol usewithin 2 hours of having sex and the number of sexual partners among a sample of African-Americanand Latino emergency department (ED patients.Methods: Cross-sectional data were collected prospectively from a randomized sample of all EDpatients during a 5-week period. In face-to-face interviews, subjects were asked to report their alcoholuse and number of sexual partners in the past 12 months. Data were analyzed using multiple variablenegative binomial regression models, and effect modification was assessed through inclusion ofinteraction terms.Results: The 395 study participants reported an average of 1.4 (standard error¼0.11 sexual partnersin the past 12 months, 23% reported misusing alcohol, and 28% reported consuming alcohol beforesex. There was no statistically significant association between alcohol misuse and the number ofsexual partners; however, alcohol before sex was associated with a larger number of sexual partners inthe past year. Moreover, among those who misused alcohol, participants who reported alcohol beforesex were 3 times more likely to report a higher number of sexual partners (risk ratio¼3.2; confidenceinterval [CI]¼1.9–5.6. The association between alcohol use before sex and number of sexual partnersis dependent upon whether a person has attributes of harmful drinking over the past 12 months.Overall, alcohol use before sex increases the number of sexual partners, but the magnitude of thiseffect is significantly increased among alcohol misusers.Conclusion: Alcohol misusers and those who reported having more than 1 sexual partner were morelikely to cluster in the same group, ie, those who used alcohol before sex. Efforts to reduce the burdenof sexually transmitted diseases, including human immunodeficiency virus, and other consequences ofrisky sexual behavior in the ED population should be cognizant of the interplay of alcohol and

  6. Ants use partner specific odors to learn to recognize a mutualistic partner.

    Directory of Open Access Journals (Sweden)

    Masaru K Hojo

    Full Text Available Regulation via interspecific communication is an important for the maintenance of many mutualisms. However, mechanisms underlying the evolution of partner communication are poorly understood for many mutualisms. Here we show, in an ant-lycaenid butterfly mutualism, that attendant ants selectively learn to recognize and interact cooperatively with a partner. Workers of the ant Pristomyrmex punctatus learn to associate cuticular hydrocarbons of mutualistic Narathura japonica caterpillars with food rewards and, as a result, are more likely to tend the caterpillars. However, the workers do not learn to associate the cuticular hydrocarbons of caterpillars of a non-ant-associated lycaenid, Lycaena phlaeas, with artificial food rewards. Chemical analysis revealed cuticular hydrocarbon profiles of the mutualistic caterpillars were complex compared with those of non-ant-associated caterpillars. Our results suggest that partner-recognition based on partner-specific chemical signals and cognitive abilities of workers are important mechanisms underlying the evolution and maintenance of mutualism with ants.

  7. Designing Comprehensive Partnering Agreements : An Introduction to the Partnering Agreement Scorecard

    NARCIS (Netherlands)

    S.M. Pfisterer (Stella); N. Payandeh (Nasim); S. Reid

    2014-01-01

    textabstractAgreements are a key mechanism of partnerships because their role is to govern interactions. They help partnerships become more effective by allowing partners to cope with relational, performance and situational risks that characterise inter-organisational relationships. The

  8. Discrepant Alcohol Use, Intimate Partner Violence, and Relationship Adjustment among Lesbian Women and their Relationship Partners.

    Science.gov (United States)

    Kelley, Michelle L; Lewis, Robin J; Mason, Tyler B

    2015-11-01

    This study examined the association between relationship adjustment and discrepant alcohol use among lesbian women and their same-sex intimate partners after controlling for verbal and physical aggression. Lesbian women (N = 819) who were members of online marketing research panels completed an online survey in which they reported both their own and same-sex intimate partner's alcohol use, their relationship adjustment, and their own and their partner's physical aggression and psychological aggression (i.e., verbal aggression and dominance/isolation). Partners' alcohol use was moderately correlated. Discrepancy in alcohol use was associated with poorer relationship adjustment after controlling for psychological aggression and physical aggression. Results are discussed in terms of the similarity and differences with previous literature primarily focused on heterosexual couples.

  9. Partner dependency and intimate partner abuse: A sociocultural grounding of spousal abuse in Ghana

    DEFF Research Database (Denmark)

    Adjei, Stephen Baffour

    2015-01-01

    While sociocultural scholarship has attempted an ecological explanation of intimate partner violence, it has largely been criticized for ignoring dispositional factors of both perpetrators and victims. Dependent personality and attachment-related emotional problems have been implicated in the ext....... It highlights Ghanaian communal personality, gendered socialization and meaning systems of marriage as salient sociocultural features for conceptualizing partner dependency and emotional-related spousal violence.......While sociocultural scholarship has attempted an ecological explanation of intimate partner violence, it has largely been criticized for ignoring dispositional factors of both perpetrators and victims. Dependent personality and attachment-related emotional problems have been implicated...... of dependency and attachment-related spousal violence as a form of a psychopathology. This article discusses partner dependency and jealousy-motivated spousal violence as socioculturally situated, dependent on contextual and relational conditions of meaning embedded in the communal society of Ghana...

  10. Plasmonic nanosensors for simultaneous quantification of multiple protein-protein binding affinities.

    Science.gov (United States)

    Ahijado-Guzmán, Rubén; Prasad, Janak; Rosman, Christina; Henkel, Andreas; Tome, Lydia; Schneider, Dirk; Rivas, Germán; Sönnichsen, Carsten

    2014-10-08

    Most of current techniques used for the quantification of protein-protein interactions require the analysis of one pair of binding partners at a time. Herein we present a label-free, simple, fast, and cost-effective route to characterize binding affinities between multiple macromolecular partners simultaneously, using optical dark-field spectroscopy and individual protein-functionalized gold nanorods as sensing elements. Our NanoSPR method could easily become a simple and standard tool in biological, biochemical, and medical laboratories.

  11. Life satisfaction and inflammation in couples: an actor-partner analysis.

    Science.gov (United States)

    Uchino, Bert N; de Grey, Robert G Kent; Cronan, Sierra; Smith, Timothy W; Diener, Ed; Joel, Samantha; Bosch, Jos

    2018-02-01

    Life satisfaction has been linked to lower cardiovascular disease mortality. However, much less is known about the biological mechanisms linking life satisfaction to physical health. In addition, the dyadic context of life satisfaction has not been considered despite increasing evidence that partners influence each other in health-relevant ways. These questions were addressed with 94 married couples who completed measures of life satisfaction and had their blood drawn for determination of interleukin-6 (IL-6) and C-reactive protein (CRP). Actor-partner models showed that higher actor levels of life satisfaction predicted lower levels of IL-6 and CRP (p's actor results were not mediated by marital satisfaction or health behaviors. Finally, no actor × partner interactions were significant and these links were not moderated by marital satisfaction. These data highlight inflammation as a potentially important biological mechanism linking actor reports of life satisfaction to lower cardiovascular mortality.

  12. Exploring the intersection of partner stalking and sexual abuse.

    Science.gov (United States)

    Logan, T K; Cole, Jennifer

    2011-07-01

    This study examined a range of sexually abusive acts women with protective orders against violent partners experienced using three groups: (a) women who never experience stalking or rape by the violent partner; (b) women who experienced stalking but who had never been raped by the violent partner; and (c) women who were stalked and raped by the violent partner. Findings suggest that women in violent relationships experienced a wide range of sexually abusive experiences and that there is a significant association of partner stalking and partner sexual abuse beyond rape. Implications for practice and future research are discussed.

  13. "Do you think your main partner has other sex partners?" A simple question provides insight into sexual risk in Jamaica.

    Science.gov (United States)

    Weir, Sharon S; Figueroa, J Peter; Byfield, Lovette L; Scott, Marion A; Hobbs, Marcia M; Edwards, Jessie E; Duncan, Jacqueline P

    2015-01-01

    To estimate the association between a simple measure of sexual partner concurrency and sexually transmitted infection (STI) we conducted a cross-sectional population-based household survey (n = 1795) and targeted surveys of people at venues where people meet sexual partners (n = 1580) to ask about sexual behaviour. Persons interviewed at venues were tested for HIV, gonorrhoea, chlamydia, and trichomoniasis. We compared the association between STI and reporting a partner had other partners. More women than men reported their main partner had other partners. Thirteen percent of all women in the population-based survey and 14.4% in the targeted survey reported having one partner in the past 12 months and that partner had additional partners. STI prevalence was significantly associated with reporting a partner had other partners (36.8% vs. 30.2%; prevalence ratio [PR] 1.2; 95% confidence interval [CI] 1.1, 1.4). Construction of complete sexual networks is costly and not routinely feasible. We recommend adding a question to cross-sectional surveys used to monitor sexual behaviour about whether the respondent believes his or her partner has other sexual partners. Although subject to bias, the question was useful in Jamaica to identify a group of women with only one sexual partner at increased risk of infection. © The Author(s) 2014 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav.

  14. Multiple partners and partner choice as risk factors for sexually transmitted disease among female college students.

    Science.gov (United States)

    Joffe, G P; Foxman, B; Schmidt, A J; Farris, K B; Carter, R J; Neumann, S; Tolo, K A; Walters, A M

    1992-01-01

    Multiple sexual partners and partner choice are believed to increase the risk of sexually transmitted disease (STD), but these behaviors had not previously been assessed outside of clinical populations. In this study, a cross-sectional survey among single, white, female students in their senior year of college was conducted to measure the association between behavioral risk factors and the acquisition of self-reported STDs during college. The usable response rate was 47.2% (n = 467). The combined prevalence of chlamydial infection, gonorrhea, genital herpes, human papillomavirus (HPV) infection, syphilis, and trichomoniasis during a 3.5-year period was 11.7%. There was a strong association between number of sexual partners and having an STD: those women with 5 or more sexual partners were 8 times more likely to report having an STD than those with only 1 partner, even after adjusting for age at first intercourse (odds ratio = 8.1; 95% confidence interval = 1.99, 32.64). The prevalence of a history of STDs increased with more causal partner choice and earlier age at first intercourse, but neither factor was independently associated with a history of STDs. Of the respondents, 23% always used condoms. Future research should focus on identifying ways of effectively changing high-risk sexual behavior.

  15. Empowerment, partner's behaviours and intimate partner physical violence among married women in Uganda.

    Science.gov (United States)

    Kwagala, Betty; Wandera, Stephen Ojiambo; Ndugga, Patricia; Kabagenyi, Allen

    2013-12-01

    There is dearth of knowledge and research about the role of empowerment, partners' behaviours and intimate partner physical violence (IPPV) among married women in Uganda. This paper examined the influence of women's empowerment and partners' behaviours on IPPV among married women in Uganda. The 2011 Uganda Demographic and Health Survey data were used, selecting a weighted sample of 1,307 women in union considered for the domestic violence module. Cross tabulations (chi-square tests) and multivariate logistic regressions were used to identify factors associated with IPPV. The prevalence of IPPV among women in union in Uganda is still high (41%). Women's occupation was the only measure of empowerment that was significantly associated with IPPV, where women in professional employment were less likely to experience IPPV. Women from wealthy households were less likely to experience IPPV. IPPV was more likely to be reported by women who had ever had children and witnessed parental IPPV. IPPV was also more likely to be reported by women whose husbands or partners: accused them of unfaithfulness, did not permit them to meet female friends, insisted on knowing their whereabouts and sometimes or often got drunk. Women who were afraid their partners were also more likely to report IPPV. In the Ugandan context, women's empowerment as assessed by the UDHS has limited mitigating effect on IPPV in the face of partners' negative behaviours and history of witnessing parental violence.

  16. Risk Factors for Intimate Partner Violence During Pregnancy and Postpartum

    Science.gov (United States)

    Hellmuth, Julianne C.; Gordon, Kristina Coop; Stuart, Gregory L.; Moore, Todd M.

    2012-01-01

    Purpose This longitudinal investigation examined potential risk factors for intimate partner violence (IPV) among women during pregnancy and 6 weeks postpartum. Methods A sample of 180 pregnant women was collected in order to investigate 1) whether associations between partner alcohol misuse, partner jealousy, partner suspicion of infidelity, and stress were associated with IPV victimization, 2) the indirect effects of alcohol misuse on these relationships, and 3) factors related to changes in IPV victimization over time. Results At baseline, partner alcohol misuse was associated with each type of IPV victimization and the combination of partner alcohol misuse, partner jealousy, and partner suspicion of infidelity was most strongly associated with severe physical victimization. Partner alcohol misuse mediated the relationship between partner jealousy and psychological and severe physical victimization. At follow-up, partner jealousy and stress were related to women’s psychological victimization and partner alcohol misuse was related to women’s severe physical victimization. Conclusions Findings suggest that partner alcohol misuse is a risk factor for women’s IPV victimization during pregnancy and jealousy and stress may increase risk for some types of IPV. Findings also suggest that intervention should target parents early in pregnancy in order to reduce the risk for future IPV. PMID:23053216

  17. Amyloid β-interacting partners in Alzheimer's disease: From accomplices to possible therapeutic targets.

    Science.gov (United States)

    Han, Sun-Ho; Park, Jong-Chan; Mook-Jung, Inhee

    2016-02-01

    Alzheimer's disease (AD) is one of the most devastating neurodegenerative diseases in modern society because of insurmountable difficulties in early diagnosis and lack of therapeutic treatments. AD pathogenesis is tightly linked to the abnormal accumulation and aggregation of amyloid β (Aβ), seemingly the main causative factor of AD; however, intensive research on Aβ has not yet explained the complexity of AD pathogenesis. Consequently, the role of other supportive partners of Aβ have been elucidated and evaluated in the etiology of AD, and their potential molecular mechanisms have emerged as possible therapeutic targets. In this review, we compile information regarding Aβ-interacting partners in normal conditions and AD pathology, and analyze their etiological roles in diverse areas. Furthermore, we integrate this information into suggestions for probable clinical applications for AD diagnosis and therapeutics. We include Aβ-interacting partners localized to the cell surface and intracellular and extracellular compartments of different cell types ranging from the central nervous system to peripheral regions. Additionally, we expand the range of Aβ-interacting partners by including not only proteins, but also inorganic substances like metals, expecting that one of these partners may yield a critical breakthrough in the field of AD diagnostics and therapeutic drug development. Copyright © 2015 Elsevier Ltd. All rights reserved.

  18. Partner Selection in Technological Licensing Agreements

    DEFF Research Database (Denmark)

    Laursen, Keld; Trombini, Giulia

    This paper investigates the matching of seller and buyer firms in the market for technology licensing. By combining organizational learning theory with the industrial organization approach, we propose a matching model in which technological similarities in terms of relatedness and familiarity work...... as attractors, and market competition and potential involuntary spillovers act as repellants. Firms seek potential licensing partners by trying to maximize technological synergies while attempting to minimize the competitive downsides. We hypothesize that when licensees engage in matches involving a partner...... with partners’ technological relatedness. We use data on the formation of license partnerships in the global biopharmaceutical industry over the period 1994-2004 and find that, on the whole, there is empirical support for our theoretical predictions....

  19. Are human resource professionals strategic business partners?

    DEFF Research Database (Denmark)

    Chiu, Randy; Selmer, Jan

    2011-01-01

    Theoretical speculations and prescriptive discussions abound in the literature regarding the strategic importance of human resource management. However, evidence based on rigorous empirical studies that the transformation from an administrative service function to strategic partnership has taken...... place has been less pervasive. Based on a sample of CEOs, line managers and HR executives, this study applies both a quantitative and a qualitative approach to examine the research question whether HR professionals are strategic business partners. Although the overall quantitative results provided...... a weak support for an affirmative answer to the research question, quantitative analyses and the qualitative findings revealed that while HR executives regarded themselves as strategic business partners, CEOs and line managers were not sure that they played this strategic business role. Plausible...

  20. Harmonic Oscillator SUSY Partners and Evolution Loops

    Directory of Open Access Journals (Sweden)

    David J. Fernández

    2012-07-01

    Full Text Available Supersymmetric quantum mechanics is a powerful tool for generating exactly solvable potentials departing from a given initial one. If applied to the harmonic oscillator, a family of Hamiltonians ruled by polynomial Heisenberg algebras is obtained. In this paper it will be shown that the SUSY partner Hamiltonians of the harmonic oscillator can produce evolution loops. The corresponding geometric phases will be as well studied.

  1. Partner Communication, Discordant Fertility Goals, and ...

    African Journals Online (AJOL)

    Participants who perceive that their partner wants fewer children also were more likely to use contraception (AOR, females = 1.8 [95% CI = 1.2-2.8]), (AOR, males = 1.9 [95% CI = 1.2-3.1]). Discussion of FP is a key determinant of contraceptive use; in couples with discordant fertility goals, pro-natalist males do not always ...

  2. Unperceived intimate partner violence and women's health.

    Science.gov (United States)

    Sonego, Michela; Gandarillas, Ana; Zorrilla, Belén; Lasheras, Luisa; Pires, Marisa; Anes, Ana; Ordobás, María

    2013-01-01

    Women who experience intimate partner violence (IPV) often do not perceive themselves as abused. This study sought to estimate the health effects of unperceived IPV (uIPV), taking violence-free women as the reference, and to compare the effects of uIPV with those of perceived IPV (pIPV). We performed a cross-sectional population study through telephone interviews of 2835 women aged 18 to 70 years living in the region of Madrid and having an ongoing intimate partner relationship or contact with a former partner in the preceding year. Based on 26 questions from the Conflict Tactics Scale-1 and the Enquête Nacional sur les Violences envers les Femmes en France and the question "Do you feel abused by your partner?" a variable was constructed in three categories, namely, the absence of IPV, uIPV and pIPV. Using logistic regression, we analyzed the association between health problems, medication use, health-service utilization and IPV (perceived and unperceived) vis-à-vis the absence of IPV. There were 247 cases of uIPV and 96 of pIPV (prevalences of 8.8% and 3.4%, respectively). The multivariate analysis showed that a substantial number of the outcomes explored were associated with uIPV, pIPV, or both. The highest odds ratios (ORs) were obtained for depression (Patient Health Questionnaire-9≥10) (uIPV: OR 3.4, 95% CI 2.4-3.8; and pIPV: 4.1, 95%CI 2.5-6.8). In most problems, the ORs did not significantly differ between the two types of IPV. uIPV is 2.6 times more frequent than pIPV and is associated with at least as many health problems as pIPV. Copyright © 2012 SESPAS. Published by Elsevier Espana. All rights reserved.

  3. Public Libraries as Partners in Astronomy Outreach

    Science.gov (United States)

    Percy, J.

    2017-09-01

    Public libraries have proven to be effective partners in bringing astronomy to audiences across the large and diverse city of Toronto, Canada, and enabling astronomers - both young and old - to interact with members of our community. This article reflects on the author's experience working with public libraries, especially the Toronto Public Library (TPL), the busiest public library system in the world, to deliver over forty public presentations.

  4. Spinal cord injury and partner relationships.

    Science.gov (United States)

    Kreuter, M

    2000-01-01

    Among the many issues confronting a newly spinal cord injured (SCI) person are apprehension about the potential impact of the acquired disability on present or future intimate relationships. To summarize the research regarding partner relationships and SCI. Medline, Psychlit and Cinahl database researches were undertaken. Several studies have focused on the issue of marital status before and after the onset of the injury. The results of the studies carried out on the prevalence of divorce are conflicting. Divorce rates have been reported to be anywhere from 8% to 48%. It appears that divorce rates tend to decline to the normal rate for the general population after the initial high risk period. Some studies have shown that marital status is a powerful predictor of independent-living outcome variables. Thus, marital stability is a concern in SCI care. The study investigating why able-bodied women might choose to marry men with a permanent physical disability, such as a SCI, showed a substantial overlap with existing models of courtship. It was also shown that a SCI person who strives to minimise the impact of the disability on a potential partner makes a more attractive candidate for a long-term relationship than an individual who has come to rely on others. Partner relationships seem to be affected by a SCI, although not as much as is widely believed. There are, however, problems interpreting the varying results of the studies due to culture differences, changes in family life in society in general and the different methodologies used. Systematic research that puts the patients' and partners' problems into perspective is necessary. Uniformity in measurement instruments would facilitate comparisons of studies.

  5. Neutrino Masses from Neutral Top Partners

    CERN Document Server

    Batell, Brian

    2015-01-01

    We present theories of `Natural Neutrinos' in which neutral fermionic top partner fields are simultaneously the right-handed neutrinos (RHN), linking seemingly disparate aspects of the Standard Model structure: a) The RHN top partners are responsible for the observed small neutrino masses, b) They help ameliorate the tuning in the weak scale and address the little hierarchy problem, and c) The factor of $3$ arising from $N_c$ in the top-loop Higgs mass corrections is countered by a factor $3$ from the number of vector-like generations of RHN. The RHN top partners may arise in pseudo-Nambu-Goldstone-Boson (pNGB) Higgs models such as the Twin Higgs, as well as more general Composite, Little, and Orbifold Higgs scenarios, and three simple example models are presented. This framework firmly predicts a TeV-scale seesaw, as the RHN masses are bounded to be below the TeV scale by naturalness. The generation of light neutrino masses relies on a collective breaking of lepton number, allowing for comparatively large ne...

  6. Patterns in PARTNERing across Public Health Collaboratives

    Directory of Open Access Journals (Sweden)

    Christine A. Bevc

    2015-10-01

    Full Text Available Inter-organizational networks represent one of the most promising practice-based approaches in public health as a way to attain resources, share knowledge, and, in turn, improve population health outcomes. However, the interdependencies and effectiveness related to the structure, management, and costs of these networks represents a critical item to be addressed. The objective of this research is to identify and determine the extent to which potential partnering patterns influence the structure of collaborative networks. This study examines data collected by PARTNER, specifically public health networks (n = 162, to better understand the structured relationships and interactions among public health organizations and their partners, in relation to collaborative activities. Combined with descriptive analysis, we focus on the composition of public health collaboratives in a series of Exponential Random Graph (ERG models to examine the partnerships between different organization types to identify the attribute-based effects promoting the formation of network ties within and across collaboratives. We found high variation within and between these collaboratives including composition, diversity, and interactions. The findings of this research suggest common and frequent types of partnerships, as well as opportunities to develop new collaborations. The result of this analysis offer additional evidence to inform and strengthen public health practice partnerships.

  7. On the Interpretation of Top Partners Searches

    CERN Document Server

    Matsedonskyi, Oleksii; Wulzer, Andrea

    2014-01-01

    Relatively light Top Partners are unmistakable signatures of reasonably Natural Composite Higgs models and as such they are worth searching for at the LHC. Their phenomenology is characterized by a certain amount of model-dependence, which makes the interpretation of Top Partner experimental searches not completely straightforward especially if one is willing to take also single production into account. We describe a model-independent strategy by which the interpretation is provided on the parameter space of a Simplified Model that captures the relevant features of all the explicit constructions. The Simplified Model limits are easy to interpret within explicit models, in a way that requires no recasting and no knowledge of the experimental details of the analyses. We illustrate the method by concrete examples, among which the searches for a charge 5/3 Partner in same-sign dileptons and the searches for a charge 2/3 singlet. In each case we perform a theory recasting of the available 8 TeV Run-1 results and a...

  8. Patterns in PARTNERing across Public Health Collaboratives.

    Science.gov (United States)

    Bevc, Christine A; Retrum, Jessica H; Varda, Danielle M

    2015-10-05

    Inter-organizational networks represent one of the most promising practice-based approaches in public health as a way to attain resources, share knowledge, and, in turn, improve population health outcomes. However, the interdependencies and effectiveness related to the structure, management, and costs of these networks represents a critical item to be addressed. The objective of this research is to identify and determine the extent to which potential partnering patterns influence the structure of collaborative networks. This study examines data collected by PARTNER, specifically public health networks (n = 162), to better understand the structured relationships and interactions among public health organizations and their partners, in relation to collaborative activities. Combined with descriptive analysis, we focus on the composition of public health collaboratives in a series of Exponential Random Graph (ERG) models to examine the partnerships between different organization types to identify the attribute-based effects promoting the formation of network ties within and across collaboratives. We found high variation within and between these collaboratives including composition, diversity, and interactions. The findings of this research suggest common and frequent types of partnerships, as well as opportunities to develop new collaborations. The result of this analysis offer additional evidence to inform and strengthen public health practice partnerships.

  9. Do aphids actively search for ant partners?

    Science.gov (United States)

    Fischer, Christophe Y; Vanderplanck, Maryse; Lognay, Georges C; Detrain, Claire; Verheggen, François J

    2015-04-01

    The aphid-ant mutualistic relationships are not necessarily obligate for neither partners but evidence is that such interactions provide them strong advantages in terms of global fitness. While it is largely assumed that ants actively search for their mutualistic partners namely using volatile cues; whether winged aphids (i.e., aphids' most mobile form) are able to select ant-frequented areas had not been investigated so far. Ant-frequented sites would indeed offer several advantages for these aphids including a lower predation pressure through ant presence and enhanced chances of establishing mutuaslistic interactions with neighbor ant colonies. In the field, aphid colonies are often observed in higher densities around ant nests, which is probably linked to a better survival ensured by ants' services. Nevertheless, this could also result from a preferential establishment of winged aphids in ant-frequented areas. We tested this last hypothesis through different ethological assays and show that the facultative myrmecophilous black bean aphid, Aphis fabae L., does not orientate its search for a host plant preferentially toward ant-frequented plants. However, our results suggest that ants reduce the number of winged aphids leaving the newly colonized plant. Thus, ants involved in facultative myrmecophilous interactions with aphids appear to contribute to structure aphid populations in the field by ensuring a better establishment and survival of newly established colonies rather than by inducing a deliberate plant selection by aphid partners based on the proximity of ant colonies. © 2014 Institute of Zoology, Chinese Academy of Sciences.

  10. National Intimate Partner and Sexual Violence Survey: 2010 Highlights

    Science.gov (United States)

    National Intimate Partner and Sexual Violence Survey CDC’s National Intimate Partner and Sexual Violence Survey (NISVS) is an ongoing, nationally-representative telephone survey that collects detailed ...

  11. National Intimate Partner and Sexual Violence Survey (NISVS)

    Data.gov (United States)

    U.S. Department of Health & Human Services — The National Intimate Partner and Sexual Violence Survey (NISVS) is an ongoing, nationally representative survey to assess experiences of intimate partner violence,...

  12. Intimate partner violence and mental health in Bolivia

    National Research Council Canada - National Science Library

    Meekers, Dominique; Pallin, Sarah C; Hutchinson, Paul

    2013-01-01

    Latin America has among the highest rates of intimate partner violence. While there is increasing evidence that intimate partner violence is associated with mental health problems, there is little such research for developing countries...

  13. 75 FR 20870 - Montreux Equity Partners II SBIC, L.P.; Montreux Equity Partners III SBIC, L.P.; Notice Seeking...

    Science.gov (United States)

    2010-04-21

    ... ADMINISTRATION Montreux Equity Partners II SBIC, L.P.; Montreux Equity Partners III SBIC, L.P.; Notice Seeking... given that Montreux Equity Partners II SBIC, L.P. and Montreux Equity Partners III SBIC, L.P., 3000 Sand...). Montreux Equity Partners III SBIC, L.P. proposes to sell part of its equity interest in Renal CarePartners...

  14. Identification and characterization of Iporin as a novel interaction partner for rab1

    Directory of Open Access Journals (Sweden)

    Konczal Magdalena

    2005-03-01

    Full Text Available Abstract Background The small GTPase rab1a and its isoform rab1b are essential regulating components in the vesicle transport between the ER and the Golgi apparatus. Rab1 is thought to act as a molecular switch and can change between an active GTP-bound and an inactive GDP-bound conformation. To elucidate the function of rab1, several approaches have been established to isolate effector proteins, which interact with the activated conformation of rab1. To date p115, GM130, golgin-84 and MICAL have been identified as direct interacting partners. Together with rab1, these molecules are components of a protein complex, which mediates and regulates intracellular vesicle transport. Results Here, we report the characterization of Iporin, which is similar to KIAA0375 as a novel rab1-interacting protein. It was initially identified by yeast two-hybrid screening experiments with the active mutant of rab1b (rab1b Q67R as bait. Iporin contains a SH3 domain and two polyproline stretches, which are known to play a role in protein/protein interactions. In addition, Iporin encloses a RUN domain, which seems to be a major part of the rab1binding domain (R1BD. Iporin is ubiquitously expressed and immunofluorescence staining displays a cytosolic punctual distribution. Interestingly, we also show that Iporin interacts with another rab1 interacting partner, the GM130 protein. Conclusion Our results demonstrate that Iporin is a potential new interacting partner of rab1. Iporin is different from already identified rab1 interacting proteins concerning protein structure and cellular localization. We conclude that Iporin might function as a link between the targeting of ER derived vesicles, triggered by the rab1 GTPase and a signaling pathway regulated by molecules containing SH3 and/or poly-proline regions. The characterization of this novel intermolecular relation could help to elucidate how vesicles find their way from ER to the Golgi apparatus.

  15. Anchored design of protein-protein interfaces.

    Directory of Open Access Journals (Sweden)

    Steven M Lewis

    Full Text Available Few existing protein-protein interface design methods allow for extensive backbone rearrangements during the design process. There is also a dichotomy between redesign methods, which take advantage of the native interface, and de novo methods, which produce novel binders.Here, we propose a new method for designing novel protein reagents that combines advantages of redesign and de novo methods and allows for extensive backbone motion. This method requires a bound structure of a target and one of its natural binding partners. A key interaction in this interface, the anchor, is computationally grafted out of the partner and into a surface loop on the design scaffold. The design scaffold's surface is then redesigned with backbone flexibility to create a new binding partner for the target. Careful choice of a scaffold will bring experimentally desirable characteristics into the new complex. The use of an anchor both expedites the design process and ensures that binding proceeds against a known location on the target. The use of surface loops on the scaffold allows for flexible-backbone redesign to properly search conformational space.This protocol was implemented within the Rosetta3 software suite. To demonstrate and evaluate this protocol, we have developed a benchmarking set of structures from the PDB with loop-mediated interfaces. This protocol can recover the correct loop-mediated interface in 15 out of 16 tested structures, using only a single residue as an anchor.

  16. Intimate Partner Violence in Nigeria Implications for Counselling ...

    African Journals Online (AJOL)

    Intimate partner violence is a social problem which continues to plague the nation. In the past, in many cultures, intimate partner violence was not viewed a serious problem. However, in recent years, it has begun to be viewed as a criminal problem. This paper explains the concepts of intimate partner violence. It discusses ...

  17. Welfare consequences for people with epilepsy and their partners

    DEFF Research Database (Denmark)

    Jennum, Poul; Sabers, Anne; Christensen, Jakob

    2017-01-01

    PURPOSE: We aimed to evaluate the excess direct and indirect costs associated with epilepsy. METHODS: From the Danish National Patient Registry (1998-2013), we identified people within all ages with an epilepsy diagnosis and matched them to control individuals. Additionally, partners of people......,494 for their partners. CONCLUSION: Epilepsy has major socioeconomic consequences for individual patients, their partners and society....

  18. 45 CFR 162.915 - Trading partner agreements.

    Science.gov (United States)

    2010-10-01

    ... 45 Public Welfare 1 2010-10-01 2010-10-01 false Trading partner agreements. 162.915 Section 162... REQUIREMENTS ADMINISTRATIVE REQUIREMENTS General Provisions for Transactions § 162.915 Trading partner agreements. A covered entity must not enter into a trading partner agreement that would do any of the...

  19. Factors Associated with Multiple-Partner Fertility among Fathers

    Science.gov (United States)

    Manlove, Jennifer; Logan, Cassandra; Ikramullah, Erum; Holcombe, Emily

    2008-01-01

    This article uses a sample of 1,731 fathers aged 16 - 45 from the 2002 National Survey of Family Growth to identify factors associated with multiple-partner fertility. Almost one third of fathers who reported multiple-partner fertility did so across a series of nonmarital relationships, and nonmarital-only multiple-partner fertility has been…

  20. Trends in Intimate Partner Violence: 1980-2008

    Science.gov (United States)

    Powers, Rachael A.; Kaukinen, Catherine Elizabeth

    2012-01-01

    Research on trends in partner violence has primarily relied on official measures of victimization focusing primarily on women's risk for intimate partner homicide. The current study uses 28 years of data from the National Crime Victimization Survey (NCVS) to examine the trends of intimate partner violence against female victims and identify…

  1. prevalence of serostatus disclosure to sexual partners among hiv

    African Journals Online (AJOL)

    2011-03-03

    Mar 3, 2011 ... felt compelled to disclose while 24.3 and 20.9% respectively disclosed to either challenge their partners to get HIV tested or with an anticipation of getting social support. Upon further probe on how the partner reacted upon disclosure, most respondents (75.7%) reported their partners had supported them.

  2. Gathering and Using Information for the Selection of Trading Partners

    NARCIS (Netherlands)

    Nijssen, E.J.; Douglas, S.P.; Calis, G.

    1999-01-01

    Examines the nature of the search process used by international firms in identifying trading partners in emerging markets, and to what extent systematic information collection on potential partners is likely to enhance the choice of satisfactory partners. The results, based on 46 Dutch companies,

  3. Partner's Stake in Conformity and Abused Wives' Psychological Trauma

    Science.gov (United States)

    DeMaris, Alfred; Kaukinen, Catherine

    2008-01-01

    This study investigates the potential buffering effect of help-seeking in the association between intimate partner assault and women's psychological trauma, and how this, in turn, may depend on the partner's stake in conformity. The sample consists of 374 women reporting the experience of domestic violence from a current intimate partner, drawn…

  4. Positive illusions about a partner's physical attractiveness and relationship quality

    NARCIS (Netherlands)

    Barelds, Dick P. H.; Dijkstra, Pieternel

    The present research examined the existence of positive illusions about a partner's physical attractiveness and its relations to relationship quality. Positive illusions were assumed to exist when individuals rated their partner as more attractive than their partner rated him or herself. In two

  5. Dyadic, Partner, and Social Network Influences on Intimate Partner Violence among Male-Male Couples

    Directory of Open Access Journals (Sweden)

    Rob Stephenson

    2013-08-01

    Full Text Available Introduction: Despite a recent focus on intimate partner violence (IPV among men who have sex with men (MSM, the male-male couple is largely absent from the IPV literature. Specifically, research on dyadic factors shaping IPV in male-male couples is lacking.Methods: We took a subsample of 403 gay/bisexual men with main partners from a 2011 survey of approximately 1,000 gay and bisexual men from Atlanta. Logistic regression models of recent (,12 month experience and perpetration of physical and sexual IPV examined dyadic factors, including racial differences, age differences, and social network characteristics of couples as key covariates shaping the reporting of IPV.Results: Findings indicate that men were more likely to report perpetration of physical violence if they were a different race to their main partner, whereas main partner age was associated with decreased reporting of physical violence. Having social networks that contained more gay friends was associated with significant reductions in the reporting of IPV, whereas having social networks comprised of sex partners or closeted gay friends was associated with increased reporting of IPV victimization and perpetration.Conclusion: The results point to several unique factors shaping the reporting of IPV within male-male couples and highlight the need for intervention efforts and prevention programs that focus on male couples, a group largely absent from both research and prevention efforts. [West J Emerg Med. 2013;14(4:316–323.

  6. Reactions to a Partner-Assisted Emotional Disclosure Intervention: Direct Observation and Self-Report of Patient and Partner Communication

    Science.gov (United States)

    Porter, Laura S.; Baucom, Donald H.; Keefe, Francis J.; Patterson, Emily S.

    2012-01-01

    Partner-assisted emotional disclosure is a couple-based intervention designed to help patients disclose cancer-related concerns to their spouses-partners. We previously found that, compared with an education/support control condition, partner-assisted emotional disclosure led to significant improvements in relationship quality and intimacy for…

  7. The Influence of Individual and Partner Characteristics on the Perpetration of Intimate Partner Violence in Young Adult Relationships

    Science.gov (United States)

    Herrera, Veronica M.; Wiersma, Jacquelyn D.; Cleveland, H. Harrington

    2008-01-01

    This study examines individual and partner characteristics associated with the perpetration of intimate partner violence (IPV) in young adult relationships with opposite sex partners. Using data from Waves 1 and 3 of the National Longitudinal Study of Adolescent Health, this study examined 1,275 young adults' heterosexual romantic relationships.…

  8. Protein-Protein Interactions in a Crowded Environment: An Analysis via Cross-Docking Simulations and Evolutionary Information

    Science.gov (United States)

    Lopes, Anne; Sacquin-Mora, Sophie; Dimitrova, Viktoriya; Laine, Elodie; Ponty, Yann; Carbone, Alessandra

    2013-01-01

    Large-scale analyses of protein-protein interactions based on coarse-grain molecular docking simulations and binding site predictions resulting from evolutionary sequence analysis, are possible and realizable on hundreds of proteins with variate structures and interfaces. We demonstrated this on the 168 proteins of the Mintseris Benchmark 2.0. On the one hand, we evaluated the quality of the interaction signal and the contribution of docking information compared to evolutionary information showing that the combination of the two improves partner identification. On the other hand, since protein interactions usually occur in crowded environments with several competing partners, we realized a thorough analysis of the interactions of proteins with true partners but also with non-partners to evaluate whether proteins in the environment, competing with the true partner, affect its identification. We found three populations of proteins: strongly competing, never competing, and interacting with different levels of strength. Populations and levels of strength are numerically characterized and provide a signature for the behavior of a protein in the crowded environment. We showed that partner identification, to some extent, does not depend on the competing partners present in the environment, that certain biochemical classes of proteins are intrinsically easier to analyze than others, and that small proteins are not more promiscuous than large ones. Our approach brings to light that the knowledge of the binding site can be used to reduce the high computational cost of docking simulations with no consequence in the quality of the results, demonstrating the possibility to apply coarse-grain docking to datasets made of thousands of proteins. Comparison with all available large-scale analyses aimed to partner predictions is realized. We release the complete decoys set issued by coarse-grain docking simulations of both true and false interacting partners, and their evolutionary

  9. STIs in sex partners notified for chlamydia exposure: implications for expedited partner therapy.

    Science.gov (United States)

    van Aar, Fleur; van Benthem, Birgit H B; van den Broek, Ingrid V F; Götz, Hannelore M

    2018-01-11

    Expedited partner therapy (EPT) may reduce chlamydia reinfection rates. However, the disadvantages of EPT for chlamydia include missing the opportunity to test for other STIs and unnecessary use of antibiotics among non-infected partners. As part of a larger study that investigated the feasibility of EPT in the Netherlands, we explored the frequency of STI among a potential EPT target population of chlamydia-notified heterosexual men and women attending STI clinics for testing. Cross-sectional national STI/HIV surveillance data, which contain information on all consultations at STI clinics, were used to calculate STI positivity rates stratified by chlamydia notification and gender, and proportions of STI that were attributable to clients notified for chlamydia. Of all consultations in 2015 (n=101 710), 14 445 (14.4%) clients reported to be notified exclusively for chlamydia. Among chlamydia-notified clients, the chlamydia positivity rate was 34.2% (n=4947), and consequently 65.8% (n=9488) of them tested negative for chlamydia. Chlamydia-notified clients contributed to 10.2% of all gonorrhoea infections (n=174/1702) and 10.9% of all infectious syphilis, HIV and/or infectious hepatitis B infections (n=15/173). Implementing EPT without additional STI testing for all partners of chlamydia-infected index patients implies that STIs other than chlamydia will be missed. Although the chlamydia positivity rate was high among chlamydia-notified partners, two-thirds would unnecessarily use azithromycin. An evaluation of EPT against the current partner treatment strategy is needed to carefully weigh the potential health gains against the potential health losses and to explore the characteristics of EPT-eligible partners. © Article author(s) (or their employer(s) unless otherwise stated in the text of the article) 2018. All rights reserved. No commercial use is permitted unless otherwise expressly granted.

  10. Associations of Partner Age Gap at Sexual Debut with Teenage Parenthood and Lifetime Number of Partners.

    Science.gov (United States)

    Masho, Saba W; Chambers, Gregory J; Wallenborn, Jordyn T; Ferrance, Jacquelyn L

    2017-06-01

    Age at sexual debut and age gap between partners at debut are modifiable characteristics that may be related to risky sexual behaviors. Understanding any such relationships is a necessary first step toward strengthening risk interventions. Age at sexual debut and partner age gap were examined for 3,154 female and 2,713 male respondents to the 2011-2013 National Survey of Family Growth who first had intercourse before age 18. Multivariable logistic regression was used to assess associations between these measures and teenage parenthood and reporting a high lifetime number of partners (i.e., a number above the sample median). Females' odds of teenage parenthood were elevated if sexual debut occurred at ages 15-17 and involved a partner age gap of 3-4 years (odds ratio, 1.8) or more (2.0); they were reduced if debut occurred before age 15 and the gap was 3-4 years (0.8). Females' likelihood of reporting a high lifetime number of partners was negatively associated with age gap (0.4-0.7, depending on age at debut and length of age gap). Males' likelihood of reporting a large number of partners was positively associated with age gap if sexual debut was before age 15 and the gap was five or more years (1.7) or if debut was at ages 15-17 and involved a 3-4-year gap (2.0). Identifying the mechanisms underlying these associations could inform program design and implementation. Copyright © 2017 by the Guttmacher Institute.

  11. Domestic violence shapes Colombian women's partner choices.

    Science.gov (United States)

    Borras-Guevara, Martha Lucia; Batres, Carlota; Perrett, David I

    2017-01-01

    Potential protection from violence has been suggested as an explanation for women's preferences for more masculine partners. Previous studies, however, have not considered that violence may be multi-modal, and hence come from different sources. Therefore, we tested the effect of different fears of violence (i.e. vulnerability to public crime, likelihood of within-partnership violence) on masculinity preferences of women from Colombia, a country known for its high rates of violence. Eighty-three adult heterosexual women (mean age ± SD = 26.7 ± 6.01) answered a survey that included questions about health (e.g. frequency of illnesses during the last year and during childhood), access to media (e.g. time spent watching television, frequency of internet use), education (i.e. highest level achieved) and violence perceptions. Participants' masculinity preferences for Salvadoran, European and Colombian male faces were recorded. Factor analysis revealed two different factors for the answers to questions related to violence. One factor loaded mostly on questions related to public violence and the second factor related to domestic violence. We found that women with higher scores on the domestic violence factor preferred significantly less masculine Colombian male faces. Even after controlling for participant age, education, access to media (TV and internet) and health-related factors, the domestic violence factor contributed significantly to explaining masculinity preferences. The results presented here suggest that women's preferences for masculinity may be a strategy to avoid aggressive partners and that the source of violence matters in mate choice. Women who perceive higher risks of domestic violence prefer less masculine looking partners. Using an experimental approach, we show that Colombian women who feel more in danger of violence within partnership prefer the faces of less masculine males. This was true even after controlling for women's education level

  12. TEP Power Partners Project [Tucson Electric Power

    Energy Technology Data Exchange (ETDEWEB)

    None, None

    2014-02-06

    The Arizona Governor’s Office of Energy Policy, in partnership with Tucson Electric Power (TEP), Tendril, and Next Phase Energy (NPE), formed the TEP Power Partners pilot project to demonstrate how residential customers could access their energy usage data and third party applications using data obtained from an Automatic Meter Reading (AMR) network. The project applied for and was awarded a Smart Grid Data Access grant through the U.S. Department of Energy. The project participants’ goal for Phase I is to actively engage 1,700 residential customers to demonstrate sustained participation, reduction in energy usage (kWh) and cost ($), and measure related aspects of customer satisfaction. This Demonstration report presents a summary of the findings, effectiveness, and customer satisfaction with the 15-month TEP Power Partners pilot project. The objective of the program is to provide residential customers with energy consumption data from AMR metering and empower these participants to better manage their electricity use. The pilot recruitment goals included migrating 700 existing customers from the completed Power Partners Demand Response Load Control Project (DRLC), and enrolling 1,000 new participants. Upon conclusion of the project on November 19, 2013; 1,390 Home Area Networks (HANs) were registered; 797 new participants installed a HAN; Survey respondents’ are satisfied with the program and found value with a variety of specific program components; Survey respondents report feeling greater control over their energy usage and report taking energy savings actions in their homes after participating in the program; On average, 43 % of the participants returned to the web portal monthly and 15% returned weekly; and An impact evaluation was completed by Opinion Dynamics and found average participant savings for the treatment period1 to be 2.3% of their household use during this period.2 In total, the program saved 163 MWh in the treatment period of 2013.

  13. Community Partners for Healthy Farming Intervention Research.

    Science.gov (United States)

    Ehlers, J; Palermo, T

    2005-05-01

    The purpose of the Community Partners for Healthy Farming Intervention Research (CPHF-IR) program is to implement and evaluate existing or new interventions for reduction of agriculture-related injuries, hazards, and illnesses. Objectives include the development of active partnerships between experienced researchers, communities, workers, managers, agricultural organizations, agribusinesses, and other stakeholders. Specific intervention projects were selected by the competitive review process in response to a request for proposals. The second series of projects (funded 2000-2003) targeted: improved ergonomics for handling grapes (CA) and for small-scale berry growers (WI, IA, MI, MN), engineering controls (KY, VA, SC) and training (IN) related to tractors, private-sector financial incentives for safety (IA, NE), and reducing eye injuries in Latino farmworkers (IL, MI, FL). Partners have provided their unique resources for accessing the target population, planning, implementation, dissemination, and evaluation. They have produced useful engineering controls, educational and motivational tools, and helped build infrastructure for promoting agricultural health as essential to sustainable agriculture. Additional outcomes have included: increased interest among participants in collaborating in further research, the feasibility of Latino lay health advisors as active partners in research, and the value of process evaluation of a partnership to enhance intervention sustainability. NIOSH is utilizing the model created for Simple Solutions: Ergonomics for Farm Workers, a document related to earlier CPHF-IR projects, for a comparable document for construction in both English and Spanish. This program has confirmed that such partnerships can produce not only sustainable interventions but also products and models with the potential to expand farther geographically than originally anticipated and even into other sectors, e.g., for primary prevention among healthcare workers and

  14. Marital Separation and Lethal Male Partner Violence.

    Science.gov (United States)

    Ellis, Desmond

    2016-05-10

    Findings reported by many researchers indicate that the association between marital separation and intimate partner femicide has achieved the status of a sociological empirical generalization. The primary objective of this article is to contribute toward the cumulative development of a conflict theoretic explanation of separation- associated femicide by creating and testing a deductive conflict resolution theory that explains the empirical generalization. The causal mechanism identified in the theory is the intensity of conflict that increases with participation in adversarial and separation and divorce proceedings. Interventions logically derived from the theory are presented in the penultimate segment. Limitations are identified in the concluding segment. © The Author(s) 2016.

  15. PARTNERS IN LEARNING NETWORK FOR UKRAINIAN TEACHERS

    Directory of Open Access Journals (Sweden)

    K. Sereda

    2011-05-01

    Full Text Available The network «Partners in Learning Network» is presented in the article – the Ukrainian segment of global educational community. PILN is created with support of the Microsoft company for teachers who use information communication technology in their professional work. The PILN's purpose and value for Ukrainian teachers, for their professional dialogue and collaboration are described in the article. Functions of PILN's communities for teacher’s cooperation, the joint decision of questions and an exchange of ideas and of technique, teaching tools for increase of level of ICT introduction in educational process are described.

  16. Jacobi photonic lattices and their SUSY partners.

    Science.gov (United States)

    Zúñiga-Segundo, A; Rodríguez-Lara, B M; Fernández C, David J; Moya-Cessa, H M

    2014-01-13

    We present a classical analog of quantum optical deformed oscillators in arrays of waveguides. The normal modes of these one-dimensional photonic crystals are given in terms of Jacobi polynomials. We show that it is possible to attack the problem via factorization by exploiting the corresponding quantum optical model. This allows us to provide an unbroken supersymmetric partner of the proposed Jacobi lattices. Thanks to the underlying SU(1, 1) group symmetry of the lattices, we present the analytic propagators and impulse functions for these one-dimensional photonic crystals.

  17. Rating managed care plans as business partners.

    Science.gov (United States)

    Barber, R L

    1997-03-01

    Managed care plans are driven to meet consumer quality of care demands. But, plans that meet these consumer demands do not necessarily meet provider expectations for quality business partnerships. Therefore, providers need to develop not only clinical performance indicators but also indicators that will help them evaluate managed care plans as business partners. Performance indicators should be developed to measure managed care plans' medical loss ratio, compensation cost/benefit, payment promptness, authorization convenience and promptness, insurance verification convenience and promptness, payment accuracy, medical management, provider relations responsiveness, member education effectiveness, recorded complaints, risk transfer, contract terms, and contract equity.

  18. The impact of partner coping in couples experiencing infertility

    DEFF Research Database (Denmark)

    Peterson, B D; Pirritano, M; Christensen, Ulla

    2008-01-01

    BACKGROUND: Most studies examining coping with infertility use the individual as the unit of analysis. Although valuable, these studies fail to show the impact that partner coping has on individual distress. Since infertility is a shared stressor, examining the impact of partner coping is particu...... the coping strategies that lead to increased and decreased partner distress.......BACKGROUND: Most studies examining coping with infertility use the individual as the unit of analysis. Although valuable, these studies fail to show the impact that partner coping has on individual distress. Since infertility is a shared stressor, examining the impact of partner coping...... as the unit of analysis. RESULTS: A partner's use of active-avoidance coping was related to the increased personal, marital and social distress for men and women. A woman's use of active-confronting coping was related to increased male marital distress. And a partner's use of meaning-based coping...

  19. Intergenerational transmission of partner violence: a 20-year prospective study.

    Science.gov (United States)

    Ehrensaft, Miriam K; Cohen, Patricia; Brown, Jocelyn; Smailes, Elizabeth; Chen, Henian; Johnson, Jeffrey G

    2003-08-01

    An unselected sample of 543 children was followed over 20 years to test the independent effects of parenting, exposure to domestic violence between parents (ETDV), maltreatment, adolescent disruptive behavior disorders, and emerging adult substance abuse disorders (SUDs) on the risk of violence to and from an adult partner. Conduct disorder (CD) was the strongest risk for perpetrating partner violence for both sexes, followed by ETDV, and power assertive punishment. The effect of child abuse was attributable to these 3 risks. ETDV conferred the greatest risk of receiving partner violence; CD increased the odds of receiving partner violence but did not mediate this effect. Child physical abuse and CD in adolescence were strong independent risks for injury to a partner. SUD mediated the effect of adolescent CD on injury to a partner but not on injury by a partner. Prevention implications are highlighted.

  20. Label-Free Proteomic Identification of Endogenous, Insulin-Stimulated Interaction Partners of Insulin Receptor Substrate-1

    Science.gov (United States)

    Geetha, Thangiah; Langlais, Paul; Luo, Moulun; Mapes, Rebekka; Lefort, Natalie; Chen, Shu-Chuan; Mandarino, Lawrence J.; Yi, Zhengping

    2011-03-01

    Protein-protein interactions are key to most cellular processes. Tandem mass spectrometry (MS/MS)-based proteomics combined with co-immunoprecipitation (CO-IP) has emerged as a powerful approach for studying protein complexes. However, a majority of systematic proteomics studies on protein-protein interactions involve the use of protein overexpression and/or epitope-tagged bait proteins, which might affect binding stoichiometry and lead to higher false positives. Here, we report an application of a straightforward, label-free CO-IP-MS/MS method, without the use of protein overexpression or protein tags, to the investigation of changes in the abundance of endogenous proteins associated with a bait protein, which is in this case insulin receptor substrate-1 (IRS-1), under basal and insulin stimulated conditions. IRS-1 plays a central role in the insulin signaling cascade. Defects in the protein-protein interactions involving IRS-1 may lead to the development of insulin resistance and type 2 diabetes. HPLC-ESI-MS/MS analyses identified eleven novel endogenous insulin-stimulated IRS-1 interaction partners in L6 myotubes reproducibly, including proteins play an important role in protein dephosphorylation [protein phosphatase 1 regulatory subunit 12A, (PPP1R12A)], muscle contraction and actin cytoskeleton rearrangement, endoplasmic reticulum stress, and protein folding, as well as protein synthesis. This novel application of label-free CO-IP-MS/MS quantification to assess endogenous interaction partners of a specific protein will prove useful for understanding how various cell stimuli regulate insulin signal transduction.

  1. Classificatory multiplicity: intimate partner violence diagnosis in emergency department consultations.

    Science.gov (United States)

    Olive, Philippa

    2017-08-01

    To explore the naming, or classification, of physical assaults by a partner as 'intimate partner violence' during emergency department consultations. Research continues to evidence instances when intimate partner physical violence is 'missed' or unacknowledged during emergency department consultations. Theoretically, this research was approached through complexity theory and the sociology of diagnosis. Research design was an applied, descriptive and explanatory, multiple-method approach that combined qualitative semistructured interviews with service-users (n = 8) and emergency department practitioners (n = 9), and qualitative and quantitative document analysis of emergency department health records (n = 28). This study found that multiple classifications of intimate partner violence were mobilised during emergency department consultations and that these different versions of intimate partner violence held different diagnostic categories, processes and consequences. The construction of different versions of intimate partner violence in emergency department consultations could explain variance in people's experiences and outcomes of consultations. The research found that the classificatory threshold for 'intimate partner violence' was too high. Strengthening systems of diagnosis (identification and intervention) so that all incidents of partner violence are named as 'intimate partner violence' would reduce the incidence of missed cases and afford earlier specialist intervention to reduce violence and limit its harms. This research found that identification of and response to intimate partner violence, even in contexts of severe physical violence, was contingent. By lowering the classificatory threshold so that all incidents of partner violence are named as 'intimate partner violence', practitioners could make a significant contribution to reducing missed intimate partner violence during consultations and improving health outcomes for this population. This

  2. Novel 5′ Fusion Partners of ETV1 and ETV4 in Prostate Cancer

    Directory of Open Access Journals (Sweden)

    Joao D. Barros-Silva

    2013-07-01

    Full Text Available Gene fusions involving the erythroblast transformation-specific (ETS transcription factors ERG, ETV1, ETV4, ETV5, and FLI1 are a common feature of prostate carcinomas (PCas. Themost common upstream fusion partner described is the androgenregulated prostate-specific gene TMPRSS2, most frequently with ERG, but additional 5′fusion partners have been described. We performed 5′ rapid amplification of cDNA ends in 18 PCas with ETV1, ETV4, or ETV5 outlier expression to identify the 5′ fusion partners. We also evaluated the exon-level expression profile of these ETS genes in 14 cases. We identified and confirmed by fluorescent in situ hybridization (FISH and reverse transcription-polymerase chain reaction the two novel chimeric genes OR51E2-ETV1 and UBTF-ETV4 in two PCas. OR51E2 encodes a G-protein.coupled receptor that is overexpressed in PCas, whereas UBTF is a ubiquitously expressed gene encoding an HMG-box DNA-binding protein involved in ribosome biogenesis. We additionally describe two novel gene fusion combinations of previously described genes, namely, SLC45A3- ETV4 and HERVK17-ETV4. Finally, we found one PCa with TMPRSS2-ETV1, one with C15orf21-ETV1, one with EST14-ETV1, and two with 14q133-q21.1-ETV1. In nine PCas (eight ETV1 and one ETV5, exhibiting ETS outlier expression and genomic rearrangement detected by FISH, no 5′ fusion partner was found. Our findings contribute significantly to characterize the heterogeneous group of ETS gene fusions and indicate that all genes described as 5′ fusion partners with one ETS gene can most likely be rearranged with any of the other ETS genes involved in prostate carcinogenesis.

  3. Novel 5' fusion partners of ETV1 and ETV4 in prostate cancer.

    Science.gov (United States)

    Barros-Silva, João D; Paulo, Paula; Bakken, Anne Cathrine; Cerveira, Nuno; Løvf, Marthe; Henrique, Rui; Jerónimo, Carmen; Lothe, Ragnhild A; Skotheim, Rolf Inge; Teixeira, Manuel R

    2013-07-01

    Gene fusions involving the erythroblast transformation-specific (ETS) transcription factors ERG, ETV1, ETV4, ETV5, and FLI1 are a common feature of prostate carcinomas (PCas). The most common upstream fusion partner described is the androgen-regulated prostate-specific gene TMPRSS2, most frequently with ERG, but additional 5' fusion partners have been described. We performed 5' rapid amplification of cDNA ends in 18 PCas with ETV1, ETV4, or ETV5 outlier expression to identify the 5' fusion partners. We also evaluated the exon-level expression profile of these ETS genes in 14 cases. We identified and confirmed by fluorescent in situ hybridization (FISH) and reverse transcription-polymerase chain reaction the two novel chimeric genes OR51E2-ETV1 and UBTF-ETV4 in two PCas. OR51E2 encodes a G-protein-coupled receptor that is overexpressed in PCas, whereas UBTF is a ubiquitously expressed gene encoding an HMG-box DNA-binding protein involved in ribosome biogenesis. We additionally describe two novel gene fusion combinations of previously described genes, namely, SLC45A3-ETV4 and HERVK17-ETV4. Finally, we found one PCa with TMPRSS2-ETV1, one with C15orf21-ETV1, one with EST14-ETV1, and two with 14q133-q21.1-ETV1. In nine PCas (eight ETV1 and one ETV5), exhibiting ETS outlier expression and genomic rearrangement detected by FISH, no 5' fusion partner was found. Our findings contribute significantly to characterize the heterogeneous group of ETS gene fusions and indicate that all genes described as 5' fusion partners with one ETS gene can most likely be rearranged with any of the other ETS genes involved in prostate carcinogenesis.

  4. Protein Crowding within the Postsynaptic Density Can Impede the Escape of Membrane Proteins

    NARCIS (Netherlands)

    Li, TP; Song, Y; Mac Gillavry, H.D.; Blanpied, TA; Raghavachari, S

    2016-01-01

    Mechanisms regulating lateral diffusion and positioning of glutamate receptors within the postsynaptic density (PSD) determine excitatory synaptic strength. Scaffold proteins in the PSD are abundant receptor binding partners, yet electron microscopy suggests that the PSD is highly crowded,

  5. Touch increases autonomic coupling between romantic partners

    Directory of Open Access Journals (Sweden)

    Jonas eChatel-Goldman

    2014-03-01

    Full Text Available Interpersonal touch is of paramount importance in human social bonding and close relationships, allowing a unique channel for affect communication. So far the effect of touch on human physiology has been studied at an individual level. The present study aims at extending the study of affective touch from isolated individuals to truly interacting dyads. We have designed an ecological paradigm where romantic partners interact only via touch and we manipulate their empathic states. Simultaneously, we collected their autonomic activity (skin conductance, pulse, respiration. 14 couples participated to the experiment. We found that interpersonal touch increased coupling of electrodermal activity between the interacting partners, regardless the intensity and valence of the emotion felt. In addition, physical touch induced strong and reliable changes in physiological states within individuals. These results support an instrumental role of interpersonal touch for affective support in close relationships. Furthermore, they suggest that touch alone allows the emergence of a somatovisceral resonance between interacting individuals, which in turn is likely to form the prerequisites for emotional contagion and empathy.

  6. An approach to non-traditional partners

    Directory of Open Access Journals (Sweden)

    Ornela Fabani

    2015-11-01

    Full Text Available The aim of this paper is to describe and analyse the evolution of the political-diplomatic and the economic and trade links between Argentina and the Gulf monarchies, as well as the factors that explain this evolution, during the administrations of Cristina Fernandez (2007-2015. Meanwhile, the premise that informs it is that that during Fernandez's governments the political and diplomatic relations between Argentina and the UAE, Saudi Arabia, Kuwait and Qatar became more dynamic as a result of the Argentine interest in strengthening economic and trade ties with those States. The deepening of these ties is explained in terms of some of the main areas of Argentina's foreign trade policy, which focus on the following: to diversify trade partners, attract investment and increase the volume of trade. Moreover, the aforementioned actors are perceived as potential partners in that the Gulf monarchies need to ensure food security and advance technological cooperation, in a context marked by the international financial crisis.

  7. Violence in childhood, attitudes about partner violence, and partner violence perpetration among men in Vietnam

    NARCIS (Netherlands)

    Yount, K.M.; Huyen, T.P.; Tran, H.M.; Krause, K.H.; Schuler, S.R.; Hoang, T.A.; VanderEnde, K.; Kramer, M.R.

    2014-01-01

    Purpose: We assess the association of men’s exposure to violence in childhood—witnessing physical violence against one’s mother and being hit or beaten by a parent or adult relative—with their attitudes about intimate partner violence (IPV) against women. We explore whether men’s perpetration of IPV

  8. A comparison of two methods of partner notification for sexually transmitted infections in South Africa: patient-delivered partner medication and patient-based partner referral

    NARCIS (Netherlands)

    Young, T.; de Kock, A.; Jones, H.; Altini, L.; Ferguson, T.; van de Wijgert, J.

    2007-01-01

    The objective of this study was to determine acceptability and feasibility of patient-based partner referral (PBPR) and patient-delivered partner medication (PDPM) among female sexually transmitted infection (STI) patients in a community-based STI screening study. Women were randomized to STI

  9. Reputation-based partner choice promotes cooperation in social networks.

    Science.gov (United States)

    Fu, Feng; Hauert, Christoph; Nowak, Martin A; Wang, Long

    2008-08-01

    We investigate the cooperation dynamics attributed to the interplay between the evolution of individual strategies and evolution of individual partnerships. We focus on the effect of reputation on an individual's partner-switching process. We assume that individuals can either change their strategies by imitating their partners or adjust their partnerships based on local information about reputations. We manipulate the partner switching in two ways; that is, individuals can switch from the lowest reputation partners, either to their partners' partners who have the highest reputation (i.e., ordering in partnership) or to others randomly chosen from the entire population (i.e., randomness in partnership). We show that when individuals are able to alter their behavioral strategies and their social interaction partnerships on the basis of reputation, cooperation can prevail. We find that the larger temptation to defect and the denser the partner network, the more frequently individuals need to shift their partnerships in order for cooperation to thrive. Furthermore, an increasing tendency of switching to partners' partners is more likely to lead to a higher level of cooperation. We show that when reputation is absent in such partner-switching processes, cooperation is much less favored than that of the reputation involved. Moreover, we investigate the effect of discounting an individual's reputation on the evolution of cooperation. Our results highlight the importance of the consideration of reputation (indirect reciprocity) on the promotion of cooperation when individuals can adjust their partnerships.

  10. Phospholipase Cβ interacts with cytosolic partners to regulate cell proliferation.

    Science.gov (United States)

    Scarlata, Suzanne; Singla, Ashima; Garwain, Osama

    2018-01-01

    Phospholipase Cβ (PLCβ) is the main effector of the Gαq signaling pathway relaying different extracellular sensory information to generate intracellular calcium signals. Besides this classic function, we have found that PLCβ plays an important but unknown role in regulating PC12 cell differentiation by interacting with components in the RNA-induced silencing machinery. In trying to understand the role of PLCβ in PC12 cell differentiation, we find that over-expressing PLCβ reduces PC12 cell proliferation while down-regulating PLCβ increases the rate of cell proliferation. However, this behavior is not seen in other cancerous cell lines. To determine the underlying mechanism, we carried out mass spectrometry analysis of PLCβ complexes in PC12 cells. We find that in unsynchronized cells, PLCβ primarily binds cyclin-dependent kinase (CDK)16 whose activity plays a key role in cell proliferation. In vitro studies show a direct association between the two proteins that result in loss in CDK16 activity. When cells are arrested in the G2/M phase, a large population of PLCβ is bound to Ago2 in a complex that contains C3PO and proteins commonly found in stress granules. Additionally, another population of PLCβ complexes with CDK18 and cyclin B1. Fluorescence lifetime imaging microscopy (FLIM) confirms cell cycle dependent associations between PLCβ and these other protein binding partners. Taken together, our studies suggest that PLCβ may play an active role in mediating interactions required to move through the cell cycle. Copyright © 2017 Elsevier Ltd. All rights reserved.

  11. Intimate partner violence and maternal educational practice.

    Science.gov (United States)

    Silva, Josianne Maria Mattos da; Lima, Marília de Carvalho; Ludermir, Ana Bernarda

    2017-04-10

    The objective of this study is to analyze the association between intimate partner violence against women and maternal educational practice directed to children at the beginning of formal education. This is a cross-sectional study, carried out between 2013 and 2014, with 631 mother/child pairs, registered in the Family Health Strategy of the Health District II of the city of Recife, State of Pernambuco, Brazil. It integrates a prospective cohort study designed to investigate the consequences of exposure to intimate partner violence in relation to the child who was born between 2005 and 2006. The maternal educational practice has been assessed by the Parent-Child Conflict Tactics Scale and the intimate partner violence by a questionnaire adapted from the Multi-Country Study on Women's Health and Domestic Violence of the World Health Organization. Intimate partner violence referred to the last 12 months and was defined by specific acts of psychological, physical, and sexual violence inflicted to women by the partner. The crude and adjusted prevalence ratios were estimated for the association studied, using log-binomial regression. The prevalence of intimate partner violence was 24.4%, and violent maternal educational practice was 93.8%. The use of non-violent discipline was mentioned by 97.6% of the women, coexisting with violent strategies of discipline. Children whose mothers reported intimate partner violence presented a higher chance of suffering psychological aggression (PR = 2.2; 95%CI 1.0-4.7). The violence suffered by the mother interferes in the parental education. The findings show high prevalence of violent maternal educational practice, pointing to the need for interventions that minimize the damage of violence in women and children. Analisar a associação entre a violência pelo parceiro íntimo contra a mulher e a prática educativa materna direcionada às crianças no início da escolaridade formal. Estudo transversal, realizado entre 2013 e 2014, com

  12. Epithelioid fibrous histiocytoma: molecular characterization of ALK fusion partners in 23 cases.

    Science.gov (United States)

    Dickson, Brendan C; Swanson, David; Charames, George S; Fletcher, Christopher Dm; Hornick, Jason L

    2018-01-12

    Epithelioid fibrous histiocytoma is a rare and distinctive cutaneous neoplasm. Most cases harbor ALK rearrangement and show ALK overexpression, which distinguish this neoplasm from conventional cutaneous fibrous histiocytoma and variants. SQSTM1 and VCL have previously been shown to partner with ALK in one case each of epithelioid fibrous histiocytoma. The purpose of this study was to examine a large cohort of epithelioid fibrous histiocytomas by next-generation sequencing to characterize the nature and prevalence of ALK fusion partners. A retrospective archival review was performed to identify cases of epithelioid fibrous histiocytoma (2012-2016). Immunohistochemistry was performed to confirm ALK expression. Targeted next-generation sequencing was applied on RNA extracted from formalin-fixed paraffin-embedded tissue to identify the fusion partners. Twenty-three cases fulfilled inclusion criteria. The mean patient age was 39 years (range, 8-74), there was no sex predilection, and >75% of cases involved the lower extremities. The most common gene fusions were SQSTM1-ALK (N=12; 52%) and VCL-ALK (N=7; 30%); the other four cases harbored novel fusion partners (DCTN1, ETV6, PPFIBP1, and SPECC1L). The pattern of ALK immunoreactivity was usually granular cytoplasmic (N=12; 52%) or granular cytoplasmic and nuclear (N=10; 43%); the case containing an ETV6 fusion partner showed nuclear staining alone. There was no apparent relationship between tumor morphology and the ALK fusion partner. In summary, SQSTM1 and VCL are the most common ALK fusion partners in epithelioid fibrous histiocytoma; DCTN1, ETV6, PPFIBP1, and SPECC1L represent rare fusion partners. The proteins encoded by these genes play diverse roles in scaffolding, cell adhesion, signaling, and transcription (among others) without clear commonalities. These findings expand the oncogenic promiscuity of many of these ALK fusion genes, which drive neoplasia in tumors of diverse lineages with widely varied clinical

  13. Trait hostility is associated with systemic inflammation in married couples: an actor-partner analysis.

    Science.gov (United States)

    Smith, Timothy W; Uchino, Bert N; Bosch, Jos A; Kent, Robert G

    2014-10-01

    Trait anger and hostility predict the development of coronary heart disease, and systemic inflammation may partly mediate this association. In a sample of 94 middle-aged and older married couples, we replicate research showing a within individuals (i.e., actor effect) association of trait hostility with high sensitivity C-reactive protein (hsCRP). As a novel extension of that research, the present study also examined the association of individuals' trait hostility with their partners' hsCRP (i.e., partner effect). Controlling for potential confounds, trait hostility, measured with the Buss-Perry Aggression Questionnaire, was significantly associated with both participants' own hsCRP, b=.0528 (SE=.0196), p=.008 and their partners' levels b=.0473 (SE=.0194), p=.016. Hence, the inflammatory correlates of trait hostility occur not only within individuals but between them, as well. The effects of unhealthy personality traits may extend to intimate partners and possibly other social network members. Copyright © 2014 Elsevier B.V. All rights reserved.

  14. 26 CFR 1.707-1 - Transactions between partner and partnership.

    Science.gov (United States)

    2010-04-01

    ... 26 Internal Revenue 8 2010-04-01 2010-04-01 false Transactions between partner and partnership. 1...) INCOME TAX (CONTINUED) INCOME TAXES Partners and Partnerships § 1.707-1 Transactions between partner and partnership. (a) Partner not acting in capacity as partner. A partner who engages in a transaction with a...

  15. Daily reports of intimate partner verbal aggression by self and partner: Short-term consequences and implications for measurement.

    Science.gov (United States)

    Derrick, Jaye L; Testa, Maria; Leonard, Kenneth E

    2014-10-01

    Agreement within couples regarding the occurrence of aggression is surprisingly low. Survey research often collapses across partners' reports to create a pooled estimate of aggression in the relationship. This method ignores possible differences in partners' perceptions of the event, potentially weakening researchers' ability to detect consequences of aggression. The current study examines both partners' reports of verbal aggression to determine whether aggression reported by only one partner influences both partners' short-term outcomes. We used a 56-day daily diary to examine the effect of verbal aggression on short-term negative outcomes. We examined whether aggression reported by either partner is sufficient to predict consequences for both partners, or if an individual must report aggression to experience consequences. Victims' reports of receiving verbal aggression were a better predictor of next day victim consequences than perpetrators' reports. Perpetrators' reports of perpetrating verbal aggression were a better predictor of next day perpetrator consequences than victims' reports. Days when partners agreed that aggression had occurred generally predicted the worst outcomes. People's own reports of verbal aggression are the best predictor of short-term consequences. Pooling partner reports of aggression may make it more difficult to understand the consequences of intimate partner aggression.

  16. Intimate partner homicide: review and implications of research and policy.

    Science.gov (United States)

    Campbell, Jacquelyn C; Glass, Nancy; Sharps, Phyllis W; Laughon, Kathryn; Bloom, Tina

    2007-07-01

    Current rates of intimate partner homicide of females are approximately 4 to 5 times the rate for male victims, although the rates for both have decreased during the past 25 years. The major risk factor for intimate partner homicide, no matter if a female or male partner is killed, is prior domestic violence. This review presents and critiques the evidence supporting the other major risk factors for intimate partner homicide in general, and for intimate partner homicide of women (femicide) in particular, namely guns, estrangement, stepchild in the home, forced sex, threats to kill, and nonfatal strangulation (choking). The demographic risk factors are also examined and the related phenomena of pregnancy-related homicide, attempted femicide, and intimate partner homicide-suicide.

  17. Preferential partner selection in an evolutionary study of prisoner's dilemma

    CERN Document Server

    Ashlock, D; Stanley, E A; Tesfatsion, L; Ashlock, Dan; Smucker, Mark D; Stanley, E Ann; Tesfatsion, Leigh

    1994-01-01

    Partner selection is an important process in many social interactions, permitting individuals to decrease the risks associated with cooperation. In large populations, defectors may escape punishment by roving from partner to partner, but defectors in smaller populations risk social isolation. We investigate these possibilities for an evolutionary prisoner's dilemma in which agents use expected payoffs to choose and refuse partners. In comparison to random or round-robin partner matching, we find that the average payoffs attained with preferential partner selection tend to be more narrowly confined to a few isolated payoff regions. Most ecologies evolve to essentially full cooperative behavior, but when agents are intolerant of defections, or when the costs of refusal and social isolation are small, we also see the emergence of wallflower ecologies in which all agents are socially isolated. In between these two extremes, we see the emergence of ecologies whose agents tend to engage in a small number of defecti...

  18. Correlates of partner abuse in male same-sex relationships.

    Science.gov (United States)

    Bartholomew, Kim; Regan, Katherine V; Oram, Doug; White, Monica A

    2008-01-01

    We investigated correlates of partner abuse in male same-sex relationships in a randomly selected community sample (N = 186). We included factors associated with abuse in heterosexual relationships, as well as factors of relevance to gay relationships. We assessed perpetration and receipt of partner abuse to examine whether variables were associated independently with abuse perpetration and/or receipt. Correlates of same-sex partner abuse were largely parallel to established correlates of heterosexual abuse. Income, education, and attachment orientation were associated with bidirectional partner abuse, and family violence and substance use were uniquely associated with victimization. Further, there were factors unique to same-sex partner abuse; HIV status and public outness were associated with bidirectional partner abuse, and internalized homophobia was uniquely associated with abuse perpetration.

  19. The psychopathic intimate partner batterer: a non-psychopathological profile

    Directory of Open Access Journals (Sweden)

    José M. Pozueco-Romero

    2014-01-01

    Full Text Available This theoretical study reviews two of the most cited profiles of intimate partner batterers in the scientific literature, paying special attention to the most notable differences between them, as well as to their common criteria. The study also discusses one of the longest standing controversies in various research studies, including the particular overview with respect to Spain: it being the constant yet erroneous reference to the equivalence of psychopathy and antisocial personality disorder. Similarly, special attention is paid to the implications of considering intimate partner batterers as having either a psychopathological or psychopathic profile, while also stressing the specific role played by psychopathy in the intimate partner batterer and, concerning psychopathic intimate partner batterers, such aspects as their specific motives for perpetrating intimate partner violence and the evaluation instruments of this particular profile. Finally, a series of future directives for research concerning psychopathic intimate partner batterers are also pointed out.

  20. Effort levels of the partners in networked manufacturing

    Science.gov (United States)

    Chai, G. R.; Cai, Z.; Su, Y. N.; Zong, S. L.; Zhai, G. Y.; Jia, J. H.

    2017-08-01

    Compared with traditional manufacturing mode, could networked manufacturing improve effort levels of the partners? What factors will affect effort level of the partners? How to encourage the partners to improve their effort levels? To answer these questions, we introduce network effect coefficient to build effort level model of the partners in networked manufacturing. The results show that (1) with the increase of the network effect in networked manufacturing, the actual effort level can go beyond the ideal level of traditional manufacturing. (2) Profit allocation based on marginal contribution rate would help improve effort levels of the partners in networked manufacturing. (3) The partners in networked manufacturing who wishes to have a larger distribution ratio must make a higher effort level, and enterprises with insufficient effort should be terminated in networked manufacturing.

  1. Strategy Guideline. Partnering for High Performance Homes

    Energy Technology Data Exchange (ETDEWEB)

    Prahl, Duncan [IBACOS, Inc., Pittsburgh, PA (United States)

    2013-01-01

    High performance houses require a high degree of coordination and have significant interdependencies between various systems in order to perform properly, meet customer expectations, and minimize risks for the builder. Responsibility for the key performance attributes is shared across the project team and can be well coordinated through advanced partnering strategies. For high performance homes, traditional partnerships need to be matured to the next level and be expanded to all members of the project team including trades, suppliers, manufacturers, HERS raters, designers, architects, and building officials as appropriate. This guide is intended for use by all parties associated in the design and construction of high performance homes. It serves as a starting point and features initial tools and resources for teams to collaborate to continually improve the energy efficiency and durability of new houses.

  2. Masturbation and Partnered Sex: Substitutes or Complements?

    Science.gov (United States)

    Regnerus, Mark; Price, Joseph; Gordon, David

    2017-10-01

    Drawing upon a large, recent probability sample of American adults ages 18-60 (7648 men and 8090 women), we explored the association between sexual frequency and masturbation, evaluating the evidence for whether masturbation compensates for unavailable sex, complements (or augments) existing paired sexual activity, or bears little association with it. We found evidence supporting a compensatory relationship between masturbation and sexual frequency for men, and a complementary one among women, but each association was both modest and contingent on how content participants were with their self-reported frequency of sex. Among men and women, both partnered status and their sexual contentment were more obvious predictors of masturbation than was recent frequency of sex. We conclude that both hypotheses as commonly evaluated suffer from failing to account for the pivotal role of subjective sexual contentment in predicting masturbation.

  3. Living with a partner with dementia

    DEFF Research Database (Denmark)

    Egilstrod, Barbara; Ravn, Maiken Bay; Petersen, Kirsten Schultz

    2018-01-01

    . The aim was to identify and synthesise qualitative studies on spouses' lived experiences of living with a partner with dementia. METHODS: A systematic search was undertaken in January 2017. Six databases (CINAHL, Cochrane Library, Embase, PubMed, PsycINFO and Sociological Abstracts) were searched, using......OBJECTIVES: Dementia causes dramatic changes in everyday-living for spouses. Occured changes in marital relationship, force spouses to perform more both mentally and physically. Leading to a spousal perceived burden. To improve understanding of spouses' needs, spouses lived experiences is needed...... search terms in accordance with PICo. A descriptive synthesis and a thematic synthesis were undertaken. FINDINGS: Fifteen studies met the inclusion criteria. Three themes derived from the analysis 1) Noticing changes in everyday life 2) Transformation to a new marital relation in everyday life...

  4. Top partner searches and Composite Higgs models

    Energy Technology Data Exchange (ETDEWEB)

    Matsedonskyi, Oleksii [DESY Hamburg (Germany); Panico, Giuliano [Barcelona Univ. Autonoma (Spain). IFAE; Wulyer, Andrea [Padova Univ. (Italy). Dipt. di Fisica e Astronomia; INFN, Padova (Italy)

    2015-12-15

    Colored fermionic partners of the top quark are well-known signatures of the Composite Higgs scenario and for this reason they have been and will be subject of an intensive experimental study at the LHC. Performing an assessment of the theoretical implications of this experimental effort is the goal of the present paper. We proceed by analyzing a set of simple benchmark models, characterized by simple two-dimensional parameter spaces where the results of the searches are conveniently visualized and their impact quantified. We only draw exclusion contours, in the hypothesis of no signal, but of course our formalism could equally well be used to report discoveries in a theoretically useful format.

  5. Top partners searches and composite Higgs models

    Energy Technology Data Exchange (ETDEWEB)

    Matsedonskyi, Oleksii [DESY,Notkestrasse 85, 22607 Hamburg (Germany); Panico, Giuliano [IFAE, Universitat Autònoma de Barcelona,E-08193 Bellaterra, Barcelona (Spain); Wulzer, Andrea [Dipartimento di Fisica e Astronomia and INFN, Sezione di Padova,Via Marzolo 8, I-35131 Padova (Italy)

    2016-04-01

    Colored fermionic partners of the top quark are well-known signatures of the Composite Higgs scenario and for this reason they have been and will be subject of an intensive experimental study at the LHC. Performing an assessment of the theoretical implications of this experimental effort is the goal of the present paper. We proceed by analyzing a set of simple benchmark models, characterized by simple two-dimensional parameter spaces where the results of the searches are conveniently visualized and their impact quantified. We only draw exclusion contours, in the hypothesis of no signal, but of course our formalism could equally well be used to report discoveries in a theoretically useful format.

  6. Effects of partner violence in migrant women

    Directory of Open Access Journals (Sweden)

    Leonor Guadalupe Delgadillo Guzmán

    2013-06-01

    Full Text Available This study analyzes the relationship between partner violence, depression and maternal self-efficacy in migrant women. It is thought that this type of violence brings about sinking morale and a hindrance for their development as mothers. It is a quantitative study of an explanatory correlational type, worked with 40 immigrant women with a residence of two to 25 years, with at least one reported episode of violation and at least one son in preschool. Three instruments were employed: “The Parental Involvement and Efficacy”, “The Survey-Depression Scale” and“The Revised Conflict Tactic Scale”. The results showed important relationships between the variables, namely, the more violence women suffer, the greater the depression is, and the greater the depression is, the lesser the maternal self-efficacy becomes.

  7. Uncertainty in Partner Selection for Virtual Enterprises

    Science.gov (United States)

    Crispim, José; de Sousa, Jorge Pinho

    A virtual enterprise (VE) is a temporary organization that pools the core competencies of its member enterprises and exploits fast changing market opportunities. The success of such an organization is strongly dependent on its composition, and the selection of partners becomes therefore a crucial issue. This problem is particularly difficult because of the uncertainties related to information, market dynamics, customer expectations and technology speed up. In this paper we propose an integrated approach to rank alternative VE configurations in business environments with uncertainty, using an extension of the TOPSIS method for fuzzy data, improved through the use of a stochastic multiobjective tabu search meta-heuristic. Preliminary computational results clearly demonstrate the potential of this approach for practical application.

  8. THE INTIMATE PARTNER VIOLENCE RESPONSIBILITY ATTRIBUTION SCALE (IPVRAS)

    OpenAIRE

    Lila, Marisol; Oliver, Amparo; Catalá-Miñana, Alba; Galiana, Laura; Gracia, Enrique

    2014-01-01

    The aim of this study is to present a psychometrically sound instrument to assess intimate partner violence offenders’ responsibility attributions: the Intimate Partner Violence Responsibility Attribution Scale. The scale was administrated to 423 adult male intimate partner violence offenders court-mandated to a community-based intervention program. A three factor structure (responsibility attribution to the legal system, responsibility attribution to the victim, and responsibility attributi...

  9. Intimate partner violence and mental health in Bolivia

    OpenAIRE

    Meekers, Dominique; Pallin, Sarah C; Hutchinson, Paul

    2013-01-01

    Background Latin America has among the highest rates of intimate partner violence. While there is increasing evidence that intimate partner violence is associated with mental health problems, there is little such research for developing countries. The purpose of this paper is to examine the relationship between Bolivian women?s experiences with physical, psychological, and sexual intimate partner violence and mental health outcomes. Methods This study analyzes data from the 2008 Bolivia Demog...

  10. Perpetration and Victimization of Intimate Partner Aggression Among Rural Mothers

    OpenAIRE

    Schwab Reese, Laura M.; Harland, Karisa; Smithart, Kelsey; Ramirez, Marizen

    2015-01-01

    Intimate partner aggression is a leading cause of injury among women of child-bearing age. Research suggests that pregnancy and the postpartum period are times of increased vulnerability to aggression. Since rural women are at an increased risk of intimate partner aggression, research is needed to examine the role of pregnancy and the presence of children on intimate partner aggression among this vulnerable population. The purpose of this study is to examine the association between young chil...

  11. Proteins and protein/surfactant mixtures at interfaces in motion

    NARCIS (Netherlands)

    Boerboom, F.J.G.

    2000-01-01

    The research described in this thesis covers a number of aspects of the relation between surface properties and foaming properties of proteins, low molecular surfactants and mixtures thereof. This work is the result of a question of the industrial partners if it is possible to understand

  12. With or without you: does partner satisfaction and partner-directed violence influence the presence of a partner on women's Facebook cover profile photographs?

    Directory of Open Access Journals (Sweden)

    Pavol Prokop

    Full Text Available Social network sites (SNSs provide new opportunities for online self-presentation of millions of users. The cover profile photograph (CPP along with written information regarding relationship status is a central component of online self-presentation, although their associations with actual romantic relationships are not clear. We investigated relationships between the presence of a current romantic partner on the CPP and the displayed relationship status and partner satisfaction, partner-directed violence and women's intrasexual competition with peers. A total of 28 % of the 158 women with a Facebook profile and being involved in a romantic relationship had their romantic partners on their CPP. As predicted, women with their partners on the CPP were more satisfied with their romantic relationship than others. Furthermore, women who did not have their partner on the CPP tended to conceal or lie in their displayed mating status suggesting that this may be a strategy how to attract another potential mate. The partner-directed violence and intrasexual competition hypotheses were not supported. Overall, the CPP and an honestly displayed relationship status is an expression of relationship satisfaction amongst women.

  13. Partnering Research Involving Mentoring and Education (PRIME) in Prostate Cancer

    National Research Council Canada - National Science Library

    Price, Marva M

    2007-01-01

    Partnering Research Involving Mentoring and Education in Prostate Cancer (PRIME) is a partnership between two nursing schools, Duke University School of Nursing and North Carolina Central University (NCCU...

  14. Partnering Research Involving Mentoring and Education (PRIME) in Prostate Cancer

    National Research Council Canada - National Science Library

    Price, Marva M

    2008-01-01

    Partnering Research Involving Mentoring and Education in Prostate Cancer (PRIME) was a partnership between two nursing schools, Duke University School of Nursing and North Carolina Central University (NCCU...

  15. Concurrent sexual partners-A predictor of Chlamydia

    DEFF Research Database (Denmark)

    Jørgensen, Marianne Johansson; Olesen, Frede; Maindal, Helle Terkildsen

    2013-01-01

    overlap between sexual partners; OR = 3.1(95% CI:1.7-5.8) - 2 overlaps between sexual partners; OR = 4.3(95% CI:1.6-13.3) - 3 overlaps between sexual partners; OR = 4.7(95% CI:1.0-43.3) Results are preliminary. Multivariate analyses will be performed and presented at the poster session. Conclusion......, but the significance of this compared with other well-known predictors has only been briefly described. Aim: The aim is to examine if concurrent partners isan independent predictor for C. trachomatis infection in young Danes aged 15-29 years. Methods: Detailed sexual behavior data were collected via a web...

  16. Seeking Nontraditional Approaches to Collaborating and Partnering with Industry

    National Research Council Canada - National Science Library

    Held, Bruce

    2002-01-01

    ...)) in January 2000, describing nontraditional approaches for the Army to follow to collaborate and partner with industry using the concepts of public- private partnerships, venture capital funding...

  17. Older partners and STD prevalence among pregnant African American teens.

    Science.gov (United States)

    Begley, Elin; Crosby, Richard A; DiClemente, Ralph J; Wingood, Gina M; Rose, Eve

    2003-03-01

    Several recent studies have addressed the question of whether adolescent females who have sex with older partners have a greater risk of sexually transmitted disease (STD) acquisition. The goal was to identify differences in STD prevalence and selected measures of behavioral risk between unmarried pregnant African American adolescent females reporting sex with older partners and those reporting sex with similar-age partners. Adolescents (n = 169) were recruited during their first prenatal visit. Adolescents completed a self-administered survey and a face-to-face interview and provided urine specimens for nucleic acid amplification assays. Approximately 65% of adolescents reported that their male sex partners were >/=2 years older, while 35% reported having similar-age male sex partners. In age-adjusted analyses, adolescents with older partners were four times more likely to test positive for chlamydia (P pregnant adolescents reporting older partners may be a priority for targeted delivery of partner services. More frequent screening for chlamydia may also be cost-effective for pregnant adolescents with older partners.

  18. Ovulatory Shifts in Womens Attractions to Primary Partners and Other Men: Further Evidence of the Importance of Primary Partner Sexual Attractiveness: e44456

    National Research Council Canada - National Science Library

    Christina M Larson; Elizabeth G Pillsworth; Martie G Haselton

    2012-01-01

      Previous research has documented shifts in women's attractions to their romantic partner and to men other than their partner across the ovulation cycle, contingent on the degree to which her partner...

  19. Ovulatory shifts in women's attractions to primary partners and other men: further evidence of the importance of primary partner sexual attractiveness

    National Research Council Canada - National Science Library

    Larson, Christina M; Pillsworth, Elizabeth G; Haselton, Martie G

    2012-01-01

    Previous research has documented shifts in women's attractions to their romantic partner and to men other than their partner across the ovulation cycle, contingent on the degree to which her partner...

  20. Romantic partners in a market perspective: expectations about what ensures a highly desirable partner.

    Science.gov (United States)

    Castro, Felipe N; Hattori, Wallisen T; Yamamoto, Maria Emília; Lopes, Fívia A

    2013-10-01

    This study used the biological market perspective and influential statistical models from the marketing field to investigate males' and females' expectations regarding which combination of characteristics are most relevant in ensuring desirable partnerships for same-sex individuals. Thus, 358 Brazilian undergraduates assessed eight descriptions of same-gender stimulus targets (formulated with different levels of physical attractiveness, social skills, and current or prospective social status) and evaluated the overall desirability of the targets' expected or probable partners. From the possible combinations, three groups emerged: for one group, mainly composed of men, status characteristics were the most important attributes; for the others, mostly composed of women, social skills or physical characteristics were identified as most important in appealing to a desirable partner. This work expands the understanding of variability in male and female romantic expectations, and its implications are discussed from an evolutionary perspective.

  1. Discrepant Alcohol Use, Intimate Partner Violence, and Relationship Adjustment among Lesbian Women and their Relationship Partners

    OpenAIRE

    Kelley, Michelle L.; Lewis, Robin J.; Mason, Tyler B.

    2015-01-01

    This study examined the association between relationship adjustment and discrepant alcohol use among lesbian women and their same-sex intimate partners after controlling for verbal and physical aggression. Lesbian women (N = 819) who were members of online marketing research panels completed an online survey in which they reported both their own and same-sex intimate partner’s alcohol use, their relationship adjustment, and their own and their partner’s physical aggression and psychological a...

  2. Human endothelial and platelet septin SEPT11: cloning of novel variants and characterisation of interaction partners.

    Science.gov (United States)

    Bartsch, Ingrid; Bläser, Susanne; Röseler, Sabrina; Sandrock, Kirstin; Busse, Anja; Huber, Michael; Rempp, Hansjörg; Lieber, Mareike; Horn, Julia; Brendle, Cornelia; Zieger, Barbara

    2010-12-01

    Septins are cytoskeletal GTPases forming heteropolymeric complexes involved in processes characterised by active membrane movement such as cytokinesis, vesicle trafficking, and exocytosis. Septins are expressed in non-mitotic cells such as neurons and platelets. SEPT11 belongs to the SEPT6 group and was identified as interaction partner of SEPT5. We cloned and characterised novel SEPT11 variants and investigated interaction partners of SEPT11 in platelets and human umbilical vein endothelial cells. An endothelial cell library was used for cloning novel SEPT11 variants. Using Northern analysis the different SEPT11 transcripts were illustrated. Interaction studies were performed using yeast two-hybrid system, precipitation, FRET, and immunofluorescence microscopy. We demonstrate that SEPT11 partners with SEPT2, SEPT4 and SEPT7 using yeast two-hybrid system and precipitation. The interaction of SEPT11 with SEPT7 is also demonstrated by FRET. In addition to the known SEPT11 transcript (SEPT11_v1) we identified a novel SEPT11 variant (SEPT11_v2) as interaction partner of SEPT4 and SEPT7. Library screening of an endothelial cell library also revealed the presence of this novel SEPT11_v2 transcript. In addition, a third SEPT11 variant (SEPT11_v3) was identified. Expression of SEPT11_v1 and of SEPT11_v2 and SEPT11_v3 in human brain regions was investigated by Northern analysis. Further interaction partners of SEPT11 are characterised using immunofluorescence. Co-localisation of SEPT2, SEPT4, SEPT7 and SEPT11 with tubulin and transferrin receptor (endocytotic marker) is demonstrated. In addition, co-localisation of SEPT4 and SEPT11 with the vesicle-associated protein synaptobrevin 1 (VAMP1), but not clearly with actin, was shown. Only SEPT2 and SEPT7 definitely co-localised with actin, but not clearly with VAMP1.

  3. Older Sexual Partners and Adolescent Females' Mental Health.

    Science.gov (United States)

    Meier, Ann; Erickson, Gina A; McLaughlin, Heather

    2016-03-01

    The physical health detriments associated with adolescent females' having older romantic partners are well documented. However, little is known about the relationship between having an older partner and females' subsequent mental health. Two waves of data from 1,440 participants in the National Longitudinal Study of Adolescent Health were analyzed. The sample was restricted to females in grades 7-12 who had not had sex at Wave 1 (1994-1995) and reported at least one romantic relationship by Wave 2 (1996). A lagged dependent variable approach with ordinary least-squares regression measured changes in depression and self-esteem associated with sexual and nonsexual relationships with same-age and older partners. Intimate partner violence was tested as a mediator. Compared with respondents reporting a nonsexual relationship with a same-age partner, those reporting a nonsexual relationship with an older partner, sex with a same-age partner or sex with an older partner experienced greater increases in depression between surveys; mean predicted depression levels at Wave 2 ranged from 7.7 to 9.0 across these groups (possible range, 0-27). Intimate partner violence explained one-third of the difference between those who had had sex and those who had not had sex with same-age partners. Fewer associations were found for self-esteem, and differences between groups were small. Health correlates of adolescent sexual behavior go beyond physical health outcomes. Future research should identify mechanisms through which relationships, especially those with older partners, are associated with declines in mental health. Copyright © 2016 by the Guttmacher Institute.

  4. Functional aspects of protein flexibility

    DEFF Research Database (Denmark)

    Teilum, Kaare; Olsen, Johan G; Kragelund, Birthe B

    2009-01-01

    Proteins are dynamic entities, and they possess an inherent flexibility that allows them to function through molecular interactions within the cell, among cells and even between organisms. Appreciation of the non-static nature of proteins is emerging, but to describe and incorporate...... this into an intuitive perception of protein function is challenging. Flexibility is of overwhelming importance for protein function, and the changes in protein structure during interactions with binding partners can be dramatic. The present review addresses protein flexibility, focusing on protein-ligand interactions....... The thermodynamics involved are reviewed, and examples of structure-function studies involving experimentally determined flexibility descriptions are presented. While much remains to be understood about protein flexibility, it is clear that it is encoded within their amino acid sequence and should be viewed...

  5. Modeling disordered protein interactions from biophysical principles

    National Research Council Canada - National Science Library

    Peterson, Lenna X; Roy, Amitava; Christoffer, Charles; Terashi, Genki; Kihara, Daisuke

    2017-01-01

    ...-protein interactions (PPIs) are formed with IDPs [3]. A well-known example is the p53 tumor suppressor, which contains disordered regions that interact with dozens of partner proteins [4]. Due to the abundance and characteristic features of IDPs in PPI networks, including many critical signaling pathways, fully understanding the molecular mechanisms of PPI networ...

  6. Effect of intimate partner violence on birth outcomes | Laelago ...

    African Journals Online (AJOL)

    Background: Violence by intimate partner during pregnancy has many adverse pregnancy outcomes. Thus, that's why we sought to determine association between intimate partner violence during pregnancy and adverse birth outcomes. Methods: A facility based cross-sectional study was conducted among 183 recently ...

  7. Intimate partners' violence in Southern Ethiopia: Examining the ...

    African Journals Online (AJOL)

    The high level of intimate partner violence (IPV) against women in many population groups in Ethiopia and the risk factors associated with the practice is not well understood among scholars and decision makers. This study examined the prevalence and risk factors associated with intimate partner violence in Sidama, ...

  8. Condom Use and Number of Sexual Partners among Secondary ...

    African Journals Online (AJOL)

    partners encourages condom use during sex among high school female students in Urban Cameroon. Methods ... normal class periods with the permission of the principals and the co-operation of the teachers concerned. ... during first sex than those with single partners, 27 (43.2%). (X2=147.5; df=6; p=0.000) (Table 3).

  9. Examining the Interface between Substance Misuse and Intimate Partner Violence

    Directory of Open Access Journals (Sweden)

    Gregory L. Stuart

    2009-01-01

    Full Text Available There is considerable theoretical and empirical support for a link between substance misuse and perpetration and victimization of intimate partner violence. This review briefly summarizes this literature and highlights current research that addresses the interface between treatment for substance abuse and intimate partner violence. Suggestions for future research and clinical implications are provided.

  10. Care Partner Responses to the Onset of Mild Cognitive Impairment

    Science.gov (United States)

    Blieszner, Rosemary; Roberto, Karen A.

    2010-01-01

    Purpose: We examined characteristics, responses, and psychological well-being of care partners who support and assist older adults recently diagnosed with mild cognitive impairment (MCI). Design and Methods: Based on a sample of 106 care partners of community residents diagnosed with MCI at memory clinics, we conducted face-to-face interviews…

  11. Pattern Of Seminal Fluid Indices Among Infertile Male Partners ...

    African Journals Online (AJOL)

    Objective: To determine the pattern of seminal fluid indices among infertile male partners attending University of Maiduguri teaching hospital infertility clinic. Method: Two hundred consecutive male partners between January- December 2003 were recruited for the study.Duration of marriage without evidence of conception ...

  12. Pattern of Semen Fluid Abnormalities in Male Partners of Infertile ...

    African Journals Online (AJOL)

    We aimed to to analyze the seminal fluid parameters of the male partners of the infertile couples managed in the hospital over a 12 month period and to identify the pattern of abnormalities. Aretrospective study of all the semen samples of male partners of infertile couples sumitted for analysis to the microbiology laboratory of ...

  13. PARTNER: A Marie Curie Initial Training Network for hadron therapy

    CERN Multimedia

    CERN BULLETIN; Nathalie Hospital; Manuela Cirilli

    2011-01-01

    PARTNER is a 4-year Marie Curie Training project funded by the European Commission with 5.6 million Euros aimed at the creation of the next generation of experts. Ten academic institutes and research centres and two leading companies are participating in PARTNER, that is coordinated by CERN, forming a unique multidisciplinary and multinational European network.

  14. Associating Pregnancy with Partner Violence against Chinese Women

    Science.gov (United States)

    Chan, Ko Ling; Brownridge, Douglas A.; Tiwari, Agnes; Fong, Daniel Y. T.; Leung, Wing Cheong; Ho, Pak Chung

    2011-01-01

    The present study discusses if pregnancy is a risk factor for intimate partner violence using a large, representative sample containing detailed information on partner violence including physical and sexual abuse as well as perpetrator-related risk factors. Data from a representative sample of 2,225 men were analyzed. The self-reported prevalence…

  15. Intimate Partner Violence: Building Resilience with Families and Children

    Science.gov (United States)

    Wortham, Thomasine T.

    2014-01-01

    Intimate partner violence includes physical, emotional, or sexual maltreatment from an intimate partner that may include name-calling, hitting, controlling behaviors, use of weapons, rape, intimidation, and a plethora of other physical and emotional tactics (Kress, Protivnak, & Sadlak, 2008; United States Department of Justice, 2013). Such…

  16. Self and partner personality and responses to relationship threats

    NARCIS (Netherlands)

    Dijkstra, Pieternel; Barelds, Dick P. H.

    2008-01-01

    Two studies examined the relations between three different types of jealousy and personality characteristics of self and partner in two large heterogeneous community samples of heterosexual couples (459 and 230 couples, respectively). It was expected that partners would resemble each other to some

  17. Psychopathology and Marital Satisfaction: The Importance of Evaluating Both Partners

    Science.gov (United States)

    Whisman, Mark A.; Uebelacker, Lisa A.; Weinstock, Lauren M.

    2004-01-01

    Using path analysis and hierarchical linear modeling, the authors evaluated the associations between both partners' level of depression and anxiety, as measured by Minnesota Multiphasic Personality Inventory-2 (MMPI-2) content scales, and both partners' level of marital satisfaction among married couples (N = 774) that participated in the MMPI…

  18. Self-Efficacy Mediates the Association between Partner Trust and ...

    African Journals Online (AJOL)

    AJRH Managing Editor

    psychological resilience and partner trust to increase understanding of trust towards one's intercourse partner. Mediation analyses .... social cognitive theory has been used to understand and influence health behaviors including condom usage14. Social cognitive theory posits that there is a dynamic interaction between ...

  19. Secondary traumatization in partners and parents of Dutch peacekeeping soldiers.

    NARCIS (Netherlands)

    Dirkzwager, A.J.E.; Bramsen, I.; Ader, H.; Ploeg, H.M. van der

    2005-01-01

    This study examines secondary traumatization among 708 partners and 332 parents of Dutch peacekeepers (i.e., personnel who participated in military actions implemented by international organizations such as the United Nations). Partners or parents of peacekeepers with 4 levels of posttraumatic

  20. Secondary traumatization in partners and parents of Dutch peacekeeping soldiers

    NARCIS (Netherlands)

    Dirkzwager, A.J.E.; Bramsen, I.; Adèr, H.J.; Ploeg, van der H.M.

    2005-01-01

    This study examines secondary traumatization among 708 partners and 332 parents of Dutch peacekeepers (i.e., personnel who participated in military actions implemented by international organizations such as the United Nations). Partners or parents of peacekeepers with 4 levels of posttraumatic

  1. The Adapted Dance Process: Planning, Partnering, and Performing

    Science.gov (United States)

    Block, Betty A.; Johnson, Peggy V.

    2011-01-01

    This article contains specific planning, partnering, and performing techniques for fully integrating dancers with special needs into a dance pedagogy program. Each aspect is discussed within the context of the domains of learning. Fundamental partnering strategies are related to each domain as part of the integration process. The authors recommend…

  2. Partnering with Big Pharma-What Academics Need to Know.

    Science.gov (United States)

    Lipton, Stuart A; Nordstedt, Christer

    2016-04-21

    Knowledge of the parameters of drug development can greatly aid academic scientists hoping to partner with pharmaceutical companies. Here, we discuss the three major pillars of drug development-pharmacodynamics, pharmacokinetics, and toxicity studies-which, in addition to pre-clinical efficacy, are critical for partnering with Big Pharma to produce novel therapeutics. Copyright © 2016 Elsevier Inc. All rights reserved.

  3. Women at Risk of Physical Intimate Partner Violence: A Cross ...

    African Journals Online (AJOL)

    HP

    information on the respondents' social and demographic characteristics, reproductive health, characteristics of current/most recent partners; attitudes towards gender roles and intimate partner violence; experience of IPV, injuries due to violence; impact and coping mechanisms used by women who experienced violence; ...

  4. Romantic Partner Selection and Socialization during Early Adolescence

    Science.gov (United States)

    Simon, Valerie A.; Aikins, Julie Wargo; Prinstein, Mitchell J.

    2008-01-01

    This prospective study examined romantic partner selection and socialization among a sample of 78 young adolescents (6th-8th graders). Independent assessments of adolescent and romantic partner adjustment were collected before and after relationships initiated via peer nomination and self-report. Prior to their relationship, adolescents and…

  5. How do we improve male partner involvement in prevention of ...

    African Journals Online (AJOL)

    Background: Recently, male partner involvement (MPI) has been advocated as a priority intervention in prevention of mother-to-child HIV transmission (PMTCT) ... in Aba, Nigeria were encouraged by way of a written invitation and phone calls to inform and invite their partners for couple HIV counseling and testing (cHCT).

  6. Religious Leaders' Perspectives on Marriage, Divorce, and Intimate Partner Violence

    Science.gov (United States)

    Levitt, Heidi M.; Ware, Kimberly N.

    2006-01-01

    Religious leaders from Jewish, Christian, and Islamic faiths were interviewed about their understanding of the intersection of intimate partner violence (IPV) and religion, and a grounded-theory analysis was conducted. The present manuscript explored the leaders' beliefs about the partners' responsibility for IPV and the role of divorce. Although…

  7. 76 FR 68745 - Seneca Power Partners, L.P.

    Science.gov (United States)

    2011-11-07

    ...] Seneca Power Partners, L.P. v. New York Independent System Operator, Inc.; Notice of Complaint Take... and Procedures, 18 CFR 385.206, Seneca Power Partners, L.P. (Complainant) filed a complaint against... TTY, call (202) 502-8659. Comment Date: 5 p.m. Eastern Time on November 28, 2011. Dated: October 28...

  8. Pattern of intimate partner violence disclosure among pregnant ...

    African Journals Online (AJOL)

    AO Ayodapo

    Background: Intimate partner violence (IPV) is a significant public health problem. Despite being a phenomenon that occurs globally, few studies have reviewed the issue of intimate partner violence among pregnant women as it relates to disclosure of abuse. This study sets out to determine the prevalence and pattern of ...

  9. Maximizing HIV partner notification opportunities for index patients ...

    African Journals Online (AJOL)

    Maximizing HIV partner notification opportunities for index patients and their sexual partners in Malawi ... contacts of an individual who has a sexually transmitted infection (STI), such as HIV, of their possible ... disease diagnosed in index patient, and greater than primary education in the index. To achieve success for ...

  10. Intimate partner violence at a tertiary institution | Spencer | South ...

    African Journals Online (AJOL)

    Background. Intimate partner violence (IPV) is actual or threatened physical, sexual, psychological, emotional or stalking abuse by an intimate partner. Despite the high prevalence of IPV in South Africa (SA), there is a paucity of data on university students training in fields where they are likely to have to manage the ...

  11. 77 FR 77070 - Black Bear Hydro Partners, LLC;

    Science.gov (United States)

    2012-12-31

    ... Energy Regulatory Commission Black Bear Hydro Partners, LLC; Notice of Intent To File License Application...-filing Process. b. Project No.: 2727-086. c. Dated Filed: October 24, 2012. d. Submitted By: Black Bear Hydro Partners, LLC (Black Bear Hydro). e. Name of Project: Ellsworth Hydroelectric Project. f. Location...

  12. Interaction Design Patterns for ePartners : Method and Example

    NARCIS (Netherlands)

    Paulissen, R.T.; Neerincx, M.A.; Cremers, A.H.M.; Kranenborg, K.

    2013-01-01

    An ePartner is a personal computer application that helps its user to achieve personal goals such as managing his or her health related behaviors concerning nutrition, exercise and medication. The aim of the first phase of the ‘ePartners that Care’ project is to achieve a general method and

  13. The crossover of burnout and its relation to partner health

    NARCIS (Netherlands)

    A.B. Bakker (Arnold)

    2009-01-01

    textabstractTwo studies among medical residents and teachers in the Netherlands and Greece tested the hypothesis that burnout may transfer from employees to their intimate partners at home and indirectly influence the partner's health. Study 1 included a general index of self-rated health, whereas,

  14. Everyday partner violence in Rwanda: The contribution of ...

    African Journals Online (AJOL)

    opperwjj

    of masculinity and the problem of gender-based violence in Rwanda, more than half of the women responded as having experienced gender-based violence committed by a partner. Cross-analyses indicate that men who went through the war and/or genocide tend to become violent towards their partners more often than ...

  15. Male partner controlling behaviour as a determinant of contraceptive ...

    African Journals Online (AJOL)

    Results show: 18.3% contraceptive prevalence among the women; and 51.6% of the women had experienced partner's being jealous if they were talking with other men. Results of the logistic regression among others show that: women whose partner's were jealous if talking with other men were 26.2% less likely to use ...

  16. The nature of qualitative construction partnering research : literature review

    NARCIS (Netherlands)

    Marieke Venselaar; Hans Warmelink

    2017-01-01

    from the publisher's site: "The purpose of this paper is to investigate the nature of qualitative construction partnering research. Design/methodology/approach. In total, 20 qualitative peer-reviewed papers about construction partnering research are reviewed. Findings: The results show four

  17. Multiple Sex Partner and Risk Behaviour Among Secondary School ...

    African Journals Online (AJOL)

    Tanzania is realizing increase in adolescents engaged in multiple sex partner behaviour and premarital sex. The objective of this study was to assess the awareness of multiple sex partner behaviour and risk factors among secondary school students in Moshi, Tanzania. Anonymously, questionnaires were completed by 360 ...

  18. HIV positive status disclosure to sexual partner among women ...

    African Journals Online (AJOL)

    HIV positive status disclosure to sexual partner among women attending ART clinic at Hawassa University Referral Hospital, SNNPR, Ethiopia. ... Conclusion: HIV positive status disclosure to sexual partner in this study was higher than what was reported in other studies in Ethiopia, for Mettu and Gore (69%) but slightly ...

  19. Collaboration in public construction - contractual incentives, partnering schemes and trust

    NARCIS (Netherlands)

    Dewulf, Geert P.M.R.; Kadefors, Anna

    2012-01-01

    It is increasingly common that public construction projects are procured and managed with explicit intentions to establish closer collaboration between partners. Opportunities for negotiation and the freedom to choose partners are more restricted in a public setting than in a private one. Thus, many

  20. What's age got to do with it? Partner age difference, power, intimate partner violence, and sexual risk in urban adolescents.

    Science.gov (United States)

    Volpe, Ellen M; Hardie, Thomas L; Cerulli, Catherine; Sommers, Marilyn S; Morrison-Beedy, Dianne

    2013-07-01

    Adolescent girls with older male main partners are at greater risk for adverse sexual health outcomes than other adolescent girls. One explanation for this finding is that low relationship power occurs with partner age difference. Using a cross-sectional, descriptive design, we investigated the effect of partner age difference between an adolescent girl and her male partner on sexual risk behavior through the mediators of sexual relationship power, and physical intimate partner violence (IPV), and psychological IPV severity. We chose Blanc's framework to guide this study as it depicts the links among demographic, social, economic, relationship, family and community characteristics, and reproductive health outcomes with gender-based relationship power and violence. Urban adolescent girls (N = 155) completed an anonymous computer-assisted self-interview survey to examine partner and relationship factors' effect on consistent condom use. Our sample had an average age of 16.1 years with a mean partner age of 17.8 years. Partners were predominantly African American (75%), non-Hispanic (74%), and low-income (81%); 24% of participants reported consistent condom use in the last 3 months. Descriptive, correlation, and multiple mediation analyses were conducted. Partner age difference was negatively associated with consistent condom use (-.4292, p psychological IPV severity) were not statistically significant. Further studies are needed to explore alternative rationale explaining the relationship between partner age differences and sexual risk factors within adolescent sexual relationships. Nonetheless, for clinicians and researchers, these findings underscore the heightened risk associated with partner age differences and impact of relationship dynamics on sexual risk behavior.

  1. Impact of male partner responses on sexual function in women with vulvodynia and their partners: a dyadic daily experience study.

    Science.gov (United States)

    Rosen, Natalie O; Bergeron, Sophie; Sadikaj, Gentiana; Glowacka, Maria; Delisle, Isabelle; Baxter, Mary-Lou

    2014-08-01

    There is a paucity of research investigating the role of interpersonal variables in vulvodynia--a prevalent, chronic, vulvo-vaginal pain condition that negatively affects many aspects of women's sexual health, emotional well-being and intimate relationships. Cross-sectional studies have shown that male partner responses to painful intercourse are associated with pain and sexual satisfaction in women with vulvodynia. Partner responses can be solicitous (attention and sympathy), negative (hostility and frustration), and facilitative (encouragement of adaptive coping). No research has assessed the influence of daily partner responses in this population. Further, there is limited knowledge regarding the impact of partner responses on sexual function, which is a key measure of impairment in vulvodynia. Using daily diaries, 66 women (M age = 27.91, SD = 5.94) diagnosed with vulvodynia and their cohabiting male partners (M age = 30.00, SD = 8.33) reported on male partner responses and sexual function on days when sexual intercourse occurred (M = 6.54, SD = 4.99). Drawing on the Actor-Partner Interdependence model (APIM), a multivariate multilevel modeling approach was adopted. A woman's sexual functioning improved on days when she perceived greater facilitative and lower solicitous and negative male partner responses, and when her male partner reported lower solicitous responses. A man's sexual functioning was poorer on days when he reported greater solicitous and negative responses. Findings suggest that facilitative male partner responses may improve sexual functioning whereas solicitous and negative responses may be detrimental. Partner responses should be targeted in psychological interventions aimed to improve the sexual functioning of affected couples.

  2. Intimate partner aggression and women's work outcomes.

    Science.gov (United States)

    LeBlanc, Manon Mireille; Barling, Julian; Turner, Nick

    2014-10-01

    Using conservation of resources theory, we examined the relationship between intimate partner aggression enacted against heterosexual women and 3 types of work-related outcomes for these women: withdrawal while at work (i.e., cognitive distraction, work neglect), withdrawal from work (i.e., partial absenteeism, intentions to quit), and performance. In Study 1, we compared withdrawal both at and from work across 3 clinically categorized groups of women (n = 50), showing that experiencing physical aggression is related to higher work neglect. We replicated and extended these findings in Study 2 using a community sample of employed women (n = 249) by considering the incremental variance explained by both physical aggression and psychological aggression on these same outcomes. Results showed that physical aggression predicted higher levels of withdrawal both at and from work, with psychological aggression predicting additional variance in partial absenteeism over and above the effects of physical aggression. Study 3 extended the model to include academic performance as an outcome in a sample of female college students (n = 122) in dating relationships. Controlling for the women's conscientiousness, psychological aggression predicted lower academic performance after accounting for the effects of physical aggression. We discuss theoretical and practical implications of these results, as well as directions for future research. (PsycINFO Database Record (c) 2014 APA, all rights reserved).

  3. [The patient--sufferer, partner or customer?].

    Science.gov (United States)

    Unschuld, Paul U

    2006-01-01

    Over the past two decades, physicians in the German healthcare system have been forced by economic and socio-political developments to accept a far-reaching dependence on non-medical interest groups. An increasing bureaucratic burden and decreasing incomes as the two most obvious signs of these changes have led to widespread unrest and dissatisfaction. Patients too have been assigned new roles. In addition to their traditional definition as "sufferers", they are now considered self-conscious customers and partners. The present paper discusses these changes focussing on the patient, thereby complementing earlier papers by the same author that have been published under the title "The Physician as a Stranger in Medicine- from driving to being driven". The perspective applied is that of a medical historian who regards the present as a product of the past. Medicine has always been asked to fulfil "demands". What is new is the nature of the "demands" that can be expected by customer-patients to be met by medicine. Furthermore, patients and not-yet-patients may constitute a pillar of future economic growth rather than a burden for a country's economy. Public health policy as well as the roles of physicians and patients are gradually adapting to these novel circumstances.

  4. Intimate partner violence: what are physicians' perceptions?

    Science.gov (United States)

    Zink, Therese; Regan, Saundra; Goldenhar, Linda; Pabst, Stephanie; Rinto, Barb

    2004-01-01

    Intimate partner violence (IPV) is common in primary care; 11% to 22% of women experienced physical abuse in the past year. Older women experience IPV as well, but it is often undetected. This study examined primary care providers' awareness about IPV in older women, including their screening practices and management. Interviews and focus groups were conducted with 44 primary care providers. Thematic analysis was used to identify common themes. Providers fell along a continuum of thoroughness for identifying and managing IPV in older women, ranging from suboptimal to thorough identification of IPV and suboptimal to thorough management of the patient. In addition to the barriers commonly reported about IPV screening in younger women, providers described limited understanding of the diagnoses commonly associated with IPV, frustration with older women's unwillingness to disclose problems and ask for help, and limited community services that accommodate older women with IPV. Providers recommended that communities sponsor public awareness campaigns about IPV as a problem for all women and that aging and IPV agencies work together. Continued provider training about IPV should include information on identifying older victims and appropriate management options. Participants stressed the importance of community efforts to raise awareness and improve resources available for older women who are victims of IPV.

  5. Partners in quality: managing your suppliers.

    Science.gov (United States)

    Conway, B A

    1991-05-01

    Just expecting more from your supplier is not what partnership is about. We have had the experience where the quality improvement and partnership banner has been waved but the tone and spirit of the meeting did not encourage or support a joint quality improvement effort. Benefits will not be achieved until the wall truly begins to come apart and the relationship is built on mutual respect and trust. Data collection and open answers to questions often reveal embarrassing errors and obvious needs for improvements. As stated before, blame and finger-pointing must be replaced with a mutual commitment to asking and answering the question, "How can we improve?" As Dr. W. Edwards Deming has stated, "End the practice of awarding business on the basis of price tag. Instead, minimize total cost. Move toward a single supplier for any one item on a long-term relationship of loyalty and trust." The structured approach of a quality improvement process and the application of quality methods and techniques has proven useful in removing emotion and helping the team focus on the process rather than the people and the issues involved. Quality improvement methods are focused on achieving both customer and supplier goals--customer satisfaction, employee satisfaction, and operational efficiency and effectiveness. Our experience with Partners in Quality as well as our experience with the quality leadership process supports a recent quote in the Harvard Business Review: "Quality is not just a slogan...(it is) the most profitable way to run a business."

  6. Trajectories of Intimate Partner Violence Victimization

    Directory of Open Access Journals (Sweden)

    Kevin M. Swartout

    2012-08-01

    Full Text Available Introduction: The purposes of this study were to assess the extent to which latent trajectories of female intimate partner violence (IPV victimization exist; and, if so, use negative childhood experiences to predict trajectory membership.Methods: We collected data from 1,575 women at 5 time-points regarding experiences during adolescence and their 4 years of college. We used latent class growth analysis to fit a series of personcentered, longitudinal models ranging from 1 to 5 trajectories. Once the best-fitting model was selected, we used negative childhood experience variables—sexual abuse, physical abuse, and witnessing domestic violence—to predict most-likely trajectory membership via multinomial logistic regression.Results: A 5-trajectory model best fit the data both statistically and in terms of interpretability. The trajectories across time were interpreted as low or no IPV, low to moderate IPV, moderate to low IPV, high to moderate IPV, and high and increasing IPV, respectively. Negative childhood experiences differentiated trajectory membership, somewhat, with childhood sexual abuse as a consistent predictor of membership in elevated IPV trajectories.Conclusion: Our analyses show how IPV risk changes over time and in different ways. These differential patterns of IPV suggest the need for prevention strategies tailored for women that consider victimization experiences in childhood and early adulthood. [West J Emerg Med. 2012;13(3:272–277.

  7. Intimate partner violence: causes and prevention.

    Science.gov (United States)

    Jewkes, Rachel

    2002-04-20

    Unlike many health problems, there are few social and demographic characteristics that define risk groups for intimate partner violence. Poverty is the exception and increases risk through effects on conflict, women's power, and male identity. Violence is used as a strategy in conflict. Relationships full of conflict, and especially those in which conflicts occur about finances, jealousy, and women's gender role transgressions are more violent than peaceful relationships. Heavy alcohol consumption also increases risk of violence. Women who are more empowered educationally, economically, and socially are most protected, but below this high level the relation between empowerment and risk of violence is non-linear. Violence is frequently used to resolve a crisis of male identity, at times caused by poverty or an inability to control women. Risk of violence is greatest in societies where the use of violence in many situations is a socially-accepted norm. Primary preventive interventions should focus on improving the status of women and reducing norms of violence, poverty, and alcohol consumption.

  8. Building Partner Cooperation between Teachers and Parents

    Directory of Open Access Journals (Sweden)

    Barbara Šteh

    2011-01-01

    Full Text Available This paper presents the goals of teacher-parent cooperation, various potential models for establishing mutual cooperation, and the conditions required to achieve quality interactive cooperation. The partnership model is highlighted as an optimal model of interactive cooperation between teachers and parents, as it includes the distribution of expertise and control with the purpose of ensuring optimal education for children. It enables the creation of an interactive working relationship in which all of those involved are respected and recognised in their efforts to achieve common goals.The second part presents the findings of an empirical study carried out on a representative sample of Slovene primary schools. Teachers (N = 467 and parents (N = 1,690 were asked to express their pinions about the need for mutual cooperation, their view of each other when fulfilling their respective roles, and where they perceive the main obstacles to mutual cooperation. It became evident that teachers and parents have doubts about each other’s competence. This does not form a solid base on which to establish and build the necessary partner relationship, and along with it mutual cooperation. Yet both groups to a large extent agree that teacher-parent cooperation is both necessary and useful. This gives rise to the question as to how to ensure that schools adopt policies promotingopportunities for better understanding, for building quality mutualrelations and for parents to become more actively involved.

  9. Intimate partner violence and pregnancy: screening and intervention.

    Science.gov (United States)

    Chisholm, Christian A; Bullock, Linda; Ferguson, James E Jef

    2017-08-01

    In the first part of this review, we provided currently accepted definitions of categories and subcategories of intimate partner violence and discussed the prevalence and health impacts of intimate partner violence in nonpregnant and pregnant women. Herein we review current recommendations for intimate partner violence screening and the evidence surrounding the effectiveness of intimate partner violence interventions. Screening for intimate partner violence may include exclusively identification of victims of intimate partner violence or both the identification of and intervention for victims. Until recently, many professional organizations did not recommend universal screening for intimate partner violence because of a lack of evidence of effectiveness of screening, lack of evidence demonstrating that screening is not harmful, and/or a lack of consensus regarding the most effective screening tool. The lack of evidence supporting an intervention posed an additional barrier to screening. The American College of Obstetricians and Gynecologists has been a staunch advocate for universal intimate partner violence screening, even when other groups either did not endorse screening or recommended it only for high-risk women. Recent published data confirm that screening is more reliable than usual care in identifying victims of intimate partner violence, both during pregnancy and in nonpregnant women. Likewise, recent published data show that there are no apparent harms of screening for intimate partner violence and that the act of screening may have an empowering effect on women and improve their relationship with and trust in their health care providers. Despite these findings, the implementation rate of intimate partner violence screening remains low. Most encouraging are the recent data showing that interventions performed after screening for intimate partner violence are effective in reducing depression symptoms and episodes of violence as well as improving some

  10. Novel protein-protein interactions inferred from literature context.

    Directory of Open Access Journals (Sweden)

    Herman H H B M van Haagen

    Full Text Available We have developed a method that predicts Protein-Protein Interactions (PPIs based on the similarity of the context in which proteins appear in literature. This method outperforms previously developed PPI prediction algorithms that rely on the conjunction of two protein names in MEDLINE abstracts. We show significant increases in coverage (76% versus 32% and sensitivity (66% versus 41% at a specificity of 95% for the prediction of PPIs currently archived in 6 PPI databases. A retrospective analysis shows that PPIs can efficiently be predicted before they enter PPI databases and before their interaction is explicitly described in the literature. The practical value of the method for discovery of novel PPIs is illustrated by the experimental confirmation of the inferred physical interaction between CAPN3 and PARVB, which was based on frequent co-occurrence of both proteins with concepts like Z-disc, dysferlin, and alpha-actinin. The relationships between proteins predicted by our method are broader than PPIs, and include proteins in the same complex or pathway. Dependent on the type of relationships deemed useful, the precision of our method can be as high as 90%. The full set of predicted interactions is available in a downloadable matrix and through the webtool Nermal, which lists the most likely interaction partners for a given protein. Our framework can be used for prioritizing potential interaction partners, hitherto undiscovered, for follow-up studies and to aid the generation of accurate protein interaction maps.

  11. Intimate partner violence and mental health in Bolivia.

    Science.gov (United States)

    Meekers, Dominique; Pallin, Sarah C; Hutchinson, Paul

    2013-06-26

    Latin America has among the highest rates of intimate partner violence. While there is increasing evidence that intimate partner violence is associated with mental health problems, there is little such research for developing countries. The purpose of this paper is to examine the relationship between Bolivian women's experiences with physical, psychological, and sexual intimate partner violence and mental health outcomes. This study analyzes data from the 2008 Bolivia Demographic and Health Survey. 10,119 married or cohabiting women ages 15-49 are included in the analysis. Probit regression models are used to assess the association between intimate partner violence and mental health, after controlling for other demographic factors and partner characteristics. The questionnaire uses selected questions from the SRQ-20 to measure symptoms of mental health problems. Intimate partner violence is common in Bolivia, with 47% of women experiencing some type of spousal abuse in the 12 months before the survey. Women exposed to physical spousal violence in the past year are more likely to experience symptoms of depression, anxiety, psychogenic non-epileptic seizures, and psychotic disorders, after controlling for other demographic and partner characteristics. Women who experienced sexual abuse by a partner are most likely to suffer from all mental health issues. Psychological abuse is also associated with an increased risk of experiencing symptoms of depression, anxiety, and psychogenic seizures. Women who experienced only psychological abuse report mental health problems similar to those who were physically abused. This study demonstrates an urgent need for research on the prevalence and health consequences of psychological abuse in developing countries. Our findings highlight the need for mental health services for victims of intimate partner violence. Because physical and psychological violence are often experienced concurrently, it is recommended that health providers

  12. Sexual discordance and sexual partnering among heterosexual women.

    Science.gov (United States)

    Nield, Jennifer; Magnusson, Brianna; Brooks, Christopher; Chapman, Derek; Lapane, Kate L

    2015-05-01

    This study examined characteristics of self-identified heterosexual women who were concordant or discordant in their sexual behavior and the association of discordance and sexual partnering among those aged 15-44 years from the 2006-2010 National Survey of Family Growth (n = 7,353). Sexual concordance was defined as reporting a heterosexual identity and no female partners in the past year; discordance was reporting a heterosexual identity and having at least one female partner in the past year. Sexual partnering was defined as being concurrent, serially monogamous or monogamous with a male partner in the previous year. Polytomous logistic regression models evaluated the association between sexual discordance and sexual partnering. Among self-identified heterosexual, sexually active women, 11.2 % reported ever having had a same sex partner. Heterosexually discordant women who had both male and female partners in the previous year were 5.5 times as likely to report having a concurrent relationship (95 % CI 2.77-11.09) and 2.4 times as likely to report engaging in serially monogamous relationships (95 % CI 1.19-4.97) with male partners. Discordance between heterosexual identity and same sex behavior is a factor in risky behaviors. Women who have sex with women and men may act as bridges for the transmission of STDs, particularly to their female partners. Sexual education should include information inclusive of non-heteronormative behaviors and identities to provide sexual minorities with the tools and information they need. Clinical guidelines should ensure that all women are offered counseling and screening for reproductive and sexual health.

  13. Computational prediction of protein interfaces : A review of data driven methods

    NARCIS (Netherlands)

    Xue, Li|info:eu-repo/dai/nl/413576817; Dobbs, Drena; Bonvin, Alexandre M J J|info:eu-repo/dai/nl/113691238; Honavar, Vasant

    2015-01-01

    Reliably pinpointing which specific amino acid residues form the interface(s) between a protein and its binding partner(s) is critical for understanding the structural and physicochemical determinants of protein recognition and binding affinity, and has wide applications in modeling and validating

  14. PARTNER INVOLVEMENT: NEGOTIATING THE PRESENCE OF PARTNERS IN PSYCHOSOCIAL ASSESSMENT AS CONDUCTED BY MIDWIVES AND CHILD AND FAMILY HEALTH NURSES.

    Science.gov (United States)

    Rollans, Mellanie; Kohlhoff, Jane; Meade, Tanya; Kemp, Lynn; Schmied, Virginia

    2016-05-01

    Universal screening for maternal depression and assessment of psychosocial risks has been integrated into the routine perinatal care provided in many Australian hospitals, but to date, partners/fathers have been largely excluded from the process. This study explored the ways in which clinicians in health service settings include partners who attend antenatal and postnatal visits with women. Qualitative data were collected using observations (n = 54), interviews (n = 60), and discussion groups (n = 7) with midwives and child and family health nurses who conducted the appointments. Transcripts from observations, interviews, and discussion groups underwent qualitative analysis, and key themes were identified. Results showed partners to have little or no involvement in psychosocial assessment and depression screening. Thematic analysis revealed four key themes: negotiating partner exclusion, partial inclusion, women's business or a couple concern? and they know anyway. Partner involvement appeared to be challenged particularly by mandatory interpersonal violence screening, which, according to health service policy, is to be conducted confidentially. Overall, results highlighted partner involvement in perinatal depression screening and psychosocial assessment processes and identified some of the benefits such as partner disclosure, but also the challenges and complexities of inclusion of partners. Clinical implications and directions for further education and research are discussed. © 2016 Michigan Association for Infant Mental Health.

  15. Partner-oriented self-regulation among bereaved parents: the costs of holding in grief for the partner's sake.

    Science.gov (United States)

    Stroebe, Margaret; Finkenauer, Catrin; Wijngaards-de Meij, Leoniek; Schut, Henk; van den Bout, Jan; Stroebe, Wolfgang

    2013-04-01

    Bereavement research has focused on individual rather than interdependent processes in coping with loss. Yet bereavement takes place in a social context, and relationship partners are likely to influence each other's grieving process. We examined the impact of a dynamic, interpersonal phenomenon, partner-oriented self-regulation (POSR): the avoidance of talking about loss and remaining strong in the partner's presence to protect the partner. Two hundred nineteen couples who had lost a child participated 6, 13, and 20 months after their loss. Consistent with predictions, results showed that one partner's POSR was associated not only with an increase in his or her own grief, but also with an increase in the other partner's grief. These relationships persisted over time: Self-reported and partner-reported POSR predicted later grief. These results are paradoxical: Although parents try to protect their partners through POSR, this effort has the opposite of the desired outcome. These findings underline the importance of further investigating interpersonal dynamics of coping with bereavement.

  16. Better than nothing? Patient-delivered partner therapy and partner notification for chlamydia: the views of Australian general practitioners

    Directory of Open Access Journals (Sweden)

    Bowden Francis J

    2010-09-01

    Full Text Available Abstract Background Genital chlamydia is the most commonly notified sexually transmissible infection (STI in Australia and worldwide and can have serious reproductive health outcomes. Partner notification, testing and treatment are important facets of chlamydia control. Traditional methods of partner notification are not reaching enough partners to effectively control transmission of chlamydia. Patient-delivered partner therapy (PDPT has been shown to improve the treatment of sexual partners. In Australia, General Practitioners (GPs are responsible for the bulk of chlamydia testing, diagnosis, treatment and follow up. This study aimed to determine the views and practices of Australian general practitioners (GPs in relation to partner notification and PDPT for chlamydia and explored GPs' perceptions of their patients' barriers to notifying partners of a chlamydia diagnosis. Methods In-depth, semi-structured telephone interviews were conducted with 40 general practitioners (GPs from rural, regional and urban Australia from November 2006 to March 2007. Topics covered: GPs' current practice and views about partner notification, perceived barriers and useful supports, previous use of and views regarding PDPT. Transcripts were imported into NVivo7 and subjected to thematic analysis. Data saturation was reached after 32 interviews had been completed. Results Perceived barriers to patients telling partners (patient referral included: stigma; age and cultural background; casual or long-term relationship, ongoing relationship or not. Barriers to GPs undertaking partner notification (provider referral included: lack of time and staff; lack of contact details; uncertainty about the legality of contacting partners and whether this constitutes breach of patient confidentiality; and feeling both personally uncomfortable and inadequately trained to contact someone who is not their patient. GPs were divided on the use of PDPT - many felt concerned that it is not

  17. Protein intrinsic disorder in plants

    Directory of Open Access Journals (Sweden)

    Florencio ePazos

    2013-09-01

    Full Text Available To some extent contradicting the classical paradigm of the relationship between protein 3D structure and function, now it is clear that large portions of the proteomes, especially in higher organisms, lack a fixed structure and still perform very important functions. Proteins completely or partially unstructured in their native (functional form are involved in key cellular processes underlain by complex networks of protein interactions. The intrinsic conformational flexibility of these disordered proteins allows them to bind multiple partners in transient interactions of high specificity and low affinity. In concordance, in plants this type of proteins has been found in processes requiring these complex and versatile interaction networks. These include transcription factor networks, where disordered proteins act as integrators of different signals or link different transcription factor subnetworks due to their ability to interact (in many cases simultaneously with different partners. Similarly, they also serve as signal integrators in signalling cascades, such as those related to response to external stimuli. Disordered proteins have also been found in plants in many stress-response processes, acting as protein chaperones or protecting other cellular components and structures. In plants, it is especially important to have complex and versatile networks able to quickly and efficiently respond to changing environmental conditions since these organisms can not escape and have no other choice than adapting to them. Consequently, protein disorder can play an especially important role in plants, providing them with a fast mechanism to obtain complex, interconnected and versatile molecular networks.

  18. Identification of semaphorin 5A interacting protein by applying apriori knowledge and peptide complementarity related to protein evolution and structure.

    Science.gov (United States)

    Sadanandam, Anguraj; Varney, Michelle L; Singh, Rakesh K

    2008-12-01

    In the post-genomic era, various computational methods that predict protein-protein interactions at the genome level are available; however, each method has its own advantages and disadvantages, resulting in false predictions. Here we developed a unique integrated approach to identify interacting partner(s) of Semaphorin 5A (SEMA5A), beginning with seven proteins sharing similar ligand interacting residues as putative binding partners. The methods include Dwyer and Root-Bernstein/Dillon theories of protein evolution, hydropathic complementarity of protein structure, pattern of protein functions among molecules, information on domain-domain interactions, co-expression of genes and protein evolution. Among the set of seven proteins selected as putative SEMA5A interacting partners, we found the functions of Plexin B3 and Neuropilin-2 to be associated with SEMA5A. We modeled the semaphorin domain structure of Plexin B3 and found that it shares similarity with SEMA5A. Moreover, a virtual expression database search and RT-PCR analysis showed co-expression of SEMA5A and Plexin B3 and these proteins were found to have co-evolved. In addition, we confirmed the interaction of SEMA5A with Plexin B3 in co-immunoprecipitation studies. Overall, these studies demonstrate that an integrated method of prediction can be used at the genome level for discovering many unknown protein binding partners with known ligand binding domains.

  19. Galectin-1 as a fusion partner for the production of soluble and folded human {beta}-1,4-galactosyltransferase-T7 in E. coli

    Energy Technology Data Exchange (ETDEWEB)

    Pasek, Marta [Structural Glycobiology Section, SAIC-Frederick, Inc., Center for Cancer Research Nanobiology Program, Center for Cancer Research, NCI-Frederick, Frederick, MD 2170 (United States); Boeggeman, Elizabeth; Ramakrishnan, Boopathy [Structural Glycobiology Section, SAIC-Frederick, Inc., Center for Cancer Research Nanobiology Program, Center for Cancer Research, NCI-Frederick, Frederick, MD 2170 (United States); Basic Science Program, SAIC-Frederick, Inc., Center for Cancer Research Nanobiology Program, Center for Cancer Research, NCI-Frederick, Frederick, MD 2170 (United States); Qasba, Pradman K., E-mail: qasba@helix.nih.gov [Structural Glycobiology Section, SAIC-Frederick, Inc., Center for Cancer Research Nanobiology Program, Center for Cancer Research, NCI-Frederick, Frederick, MD 2170 (United States)

    2010-04-09

    The expression of recombinant proteins in Escherichia coli often leads to inactive aggregated proteins known as the inclusion bodies. To date, the best available tool has been the use of fusion tags, including the carbohydrate-binding protein; e.g., the maltose-binding protein (MBP) that enhances the solubility of recombinant proteins. However, none of these fusion tags work universally with every partner protein. We hypothesized that galectins, which are also carbohydrate-binding proteins, may help as fusion partners in folding the mammalian proteins in E. coli. Here we show for the first time that a small soluble lectin, human galectin-1, one member of a large galectin family, can function as a fusion partner to produce soluble folded recombinant human glycosyltransferase, {beta}-1,4-galactosyltransferase-7 ({beta}4Gal-T7), in E. coli. The enzyme {beta}4Gal-T7 transfers galactose to xylose during the synthesis of the tetrasaccharide linker sequence attached to a Ser residue of proteoglycans. Without a fusion partner, {beta}4Gal-T7 is expressed in E. coli as inclusion bodies. We have designed a new vector construct, pLgals1, from pET-23a that includes the sequence for human galectin-1, followed by the Tev protease cleavage site, a 6x His-coding sequence, and a multi-cloning site where a cloned gene is inserted. After lactose affinity column purification of galectin-1-{beta}4Gal-T7 fusion protein, the unique protease cleavage site allows the protein {beta}4Gal-T7 to be cleaved from galectin-1 that binds and elutes from UDP-agarose column. The eluted protein is enzymatically active, and shows CD spectra comparable to the folded {beta}4Gal-T1. The engineered galectin-1 vector could prove to be a valuable tool for expressing other proteins in E. coli.

  20. [Health status and intimate partner violence].

    Science.gov (United States)

    Sanz-Barbero, Belén; Rey, Lourdes; Otero-García, Laura

    2014-01-01

    To describe the prevalence of intimate partner violence (IPV) in Spain in the last year and at some point during the lifetime, to determine health status in women according to whether they had experienced IPV or not, and to analyze the individual variables associated with IPV in Spain. A cross-sectional study was performed of the database, Macrosurvey on Gender Violence in Spain 2011. This database includes data on 7,898 women older than 18 years old. The dependent variables were IPV-last year, IPV-ever in life. Covariates consisted of sociodemographic characteristics, socioeconomic status, maternal experience of IPV, social support, and self-care. The measure of association used was the OR with its 95% confidence interval (95% CI). A total of 3.6% of women had experienced IPV-last year and 12.2% ever in life. Female victims of IPV had poorer health than women who had not experienced IPV. Immigrant women living in Spain for 6 years or more were more likely to experience IPV-ever in life than Spanish women [OR (95% CI): 1.95 (1.50, 2.53)]. An interaction was found between nationality and the existence of children under 18 years old. Among women with children under 18 years old, immigrant women were more likely to experience IPV-last year than Spanish women [OR (95% CI): 1.99 (1.25, 3.17)]. Other variables associated with IPV were age, low socioeconomic status, low social support and having a mother who had experienced IPV. In Spain, some women have a higher probability of experiencing IPV. The variables associated with greater vulnerability to IPV should be taken into account when implementing measures to prevent or alleviate IPV. Copyright © 2013 SESPAS. Published by Elsevier Espana. All rights reserved.

  1. Role of PINCH and its partner tumor suppressor Rsu-1 in regulating liver size and tumorigenesis.

    Directory of Open Access Journals (Sweden)

    Shashikiran Donthamsetty

    Full Text Available Particularly interesting new cysteine-histidine-rich protein (PINCH protein is part of the ternary complex known as the IPP (integrin linked kinase (ILK-PINCH-Parvin-α complex. PINCH itself binds to ILK and to another protein known as Rsu-1 (Ras suppressor 1. We generated PINCH 1 and PINCH 2 Double knockout mice (referred as PINCH DKO mice. PINCH2 elimination was systemic whereas PINCH1 elimination was targeted to hepatocytes. The genetically modified mice were born normal. The mice were sacrificed at different ages after birth. Soon after birth, they developed abnormal hepatic histology characterized by disorderly hepatic plates, increased proliferation of hepatocytes and biliary cells and increased deposition of extracellular matrix. After a sustained and prolonged proliferation of all epithelial components, proliferation subsided and final liver weight by the end of 30 weeks in livers with PINCH DKO deficient hepatocytes was 40% larger than the control mice. The livers of the PINCH DKO mice were also very stiff due to increased ECM deposition throughout the liver, with no observed nodularity. Mice developed liver cancer by one year. These mice regenerated normally when subjected to 70% partial hepatectomy and did not show any termination defect. Ras suppressor 1 (Rsu-1 protein, the binding partner of PINCH is frequently deleted in human liver cancers. Rsu-1 expression is dramatically decreased in PINCH DKO mouse livers. Increased expression of Rsu-1 suppressed cell proliferation and migration in HCC cell lines. These changes were brought about not by affecting activation of Ras (as its name suggests but by suppression of Ras downstream signaling via RhoGTPase proteins. In conclusion, our studies suggest that removal of PINCH results in enlargement of liver and tumorigenesis. Decreased levels of Rsu-1, a partner for PINCH and a protein often deleted in human liver cancer, may play an important role in the development of the observed phenotype.

  2. Reasons for intimate partner violence perpetration among arrested women.

    Science.gov (United States)

    Stuart, Gregory L; Moore, Todd M; Gordon, Kristina Coop; Hellmuth, Julianne C; Ramsey, Susan E; Kahler, Christopher W

    2006-07-01

    There are limited empirical data regarding the reasons or motives for the perpetration of intimate partner violence among women arrested for domestic violence and court referred to violence intervention programs. The present study examined arrested women's self-report reasons for partner violence perpetration and investigated whether women who were victims of severe intimate partner violence were more likely than were women who were victims of minor partner violence to report self-defense as a reason for their behavior. In all, 87 women in violence intervention programs completed a measure of violence perpetration and victimization and a questionnaire assessing 29 reasons for violence perpetration. Self-defense, poor emotion regulation, provocation by the partner, and retaliation for past abuse were the most common reasons for violence perpetration. Victims of severe partner violence were significantly more likely than were victims of minor partner violence to report self-defense as a reason for their violence perpetration. The clinical implications of these findings are discussed.

  3. The moderating role of partner support among smokers and abstainers

    Directory of Open Access Journals (Sweden)

    Maria da Graça Pereira

    2014-05-01

    Full Text Available Objective: To analyze the moderating effect of partner support in the relationship between psychological morbidity and quality of life among smokers and abstainers. Methods: This observational cross-sectional study assessed “partner support”, “psychological morbidity” (depression, anxiety and stress and “quality of life” in a convenience sample of 224 smokers and 169 abstainers through questionnaires that were validated and analyzed using regression and simple slopes. Results: Partner support (positive and negative among smokers appeared as a moderator in the relationship between psychological morbidity and physical quality of life. As for mental quality of life, the partner support did not have a moderating effect. The results among abstainers are similar to those found among smokers. When positive partner support is strong, there is an opposite relationship between psychological morbidity and quality of life. However, this relationship is stronger when negative partner support is strong and, in this case, the relationship between psychological morbidity and quality of life is negative. Conclusion: Partner support, whether positive or negative, was an important moderator concerning quality of life and psychological morbidity of smokers and abstainers. doi:10.5020/18061230.2013.p349

  4. Women's experience of intimate partner violence in Haiti.

    Science.gov (United States)

    Gage, Anastasia J

    2005-07-01

    This study examined individual, partner, and community characteristics associated with the occurrence of intimate partner violence among ever-married women of reproductive age, using data from the 2000 Haiti Demographic and Health Survey. Separate logistic regressions were analyzed to assess women's risks of experiencing emotional, physical and sexual violence and multiple forms of intimate partner violence in the past 12 months. Twenty-nine percent of women in the sample experienced some form of intimate partner violence in the past 12 months, with 13 percent having experienced at least two different forms of violence. Significant positive associations with all forms of violence were found for lack of completion of primary school, history of violence exposure in women's families of origin either through witnessing violence between parents while growing up or direct experience of physical violence perpetrated by family members, partner's jealousy, partner's need for control, partner's history of drunkenness, and female-dominated financial decision-making. Significant positive associations were found between men's physical abuse of children at the community level and women's risk of experiencing emotional and physical violence. Neighborhood poverty and male unemployment, number of children living at home, women's attitudinal acceptance of wife beating, and male-dominated financial decision-making were additional risk factors for sexual violence. Women's economic independence was a protective factor for emotional and physical violence, while relationship quality was protective for all forms of violence and multiple victimizations.

  5. Heteronormativity and sexual partnering among bisexual Latino men.

    Science.gov (United States)

    Muñoz-Laboy, Miguel; Garcia, Jonathan; Wilson, Patrick A; Parker, Richard G; Severson, Nicolette

    2015-05-01

    Our analyses address the question of how bisexual Latino men organize their sexual partnerships. Heteronormativity can be understood as the set of social norms and normative structures that guide sexual partnering among men and women. We provide descriptive statistics to describe bisexual Latino men's sexual partnerships. Logistic and linear regression modeling were used to explore bivariate and multivariate relationships. Of our total sample (N = 142), 41.6 % had unprotected vaginal intercourse 2 months prior to the interview; 21.8 % had unprotected anal intercourse with female partners; 37.5 % had unprotected insertive anal intercourse with male partners; and 22.5 % had unprotected receptive anal intercourse with male partners. In our multivariate model, machismo was directly associated with meeting female partners through formal spaces (workplace, school, and/or church), but inversely associated with meeting male partners in formal spaces. Machismo was positively associated with meeting male sex partners through social networks (i.e., friendship and kinship networks). The more comfortable men were with homosexuality the less likely they were to meet men online and the more likely they were to meet men through social networks of friends and kinship. Interventions to reduce sexually transmitted diseases that target bisexual behavior as an epidemiological "bridge" of transmission from homosexual to heterosexual networks might very well benefit from a more complex understanding of how Latino bisexuality is patterned. Thus, this exploratory analysis might lead to a rethinking of how to address risk and vulnerability among Latino bisexual men and their sexual networks.

  6. HIV protein sequence hotspots for crosstalk with host hub proteins.

    Directory of Open Access Journals (Sweden)

    Mahdi Sarmady

    Full Text Available HIV proteins target host hub proteins for transient binding interactions. The presence of viral proteins in the infected cell results in out-competition of host proteins in their interaction with hub proteins, drastically affecting cell physiology. Functional genomics and interactome datasets can be used to quantify the sequence hotspots on the HIV proteome mediating interactions with host hub proteins. In this study, we used the HIV and human interactome databases to identify HIV targeted host hub proteins and their host binding partners (H2. We developed a high throughput computational procedure utilizing motif discovery algorithms on sets of protein sequences, including sequences of HIV and H2 proteins. We identified as HIV sequence hotspots those linear motifs that are highly conserved on HIV sequences and at the same time have a statistically enriched presence on the sequences of H2 proteins. The HIV protein motifs discovered in this study are expressed by subsets of H2 host proteins potentially outcompeted by HIV proteins. A large subset of these motifs is involved in cleavage, nuclear localization, phosphorylation, and transcription factor binding events. Many such motifs are clustered on an HIV sequence in the form of hotspots. The sequential positions of these hotspots are consistent with the curated literature on phenotype altering residue mutations, as well as with existing binding site data. The hotspot map produced in this study is the first global portrayal of HIV motifs involved in altering the host protein network at highly connected hub nodes.

  7. Psychological Distress among Individuals Whose Partners Have Cancer.

    Science.gov (United States)

    Nakaya, Naoki; Sone, Toshimasa; Nakaya, Kumi; Tomata, Yasutake; Hozawa, Atsushi; Tsuji, Ichiro

    2017-09-01

    Cancer diagnosis influences both patients and their closest relatives. This cross-sectional study examined psychological distress among individuals whose partners had cancer in a population-based sample. Participants in the survey were citizens residing in Ohsaki City, Miyagi, Japan. Spouse pairs were identified by information of participants' relationship to the householder and address provided by municipality, and we collected self-report information on cancer history and current pain (but not the cause of pain). Psychological distress was evaluated using the Kessler 6 scale (K6). We identified 29,410 potential participants (14,705 couples), of which 23,766 (11,690 men and 12,076 women) were included in the analyses. A total of 1,374 participants (581 male and 793 female participants) had partners with history of cancer. Logistic regression analyses revealed that these participants, regardless of sex, did not show significantly higher risk of psychological distress (K6 score ≥ 13). When stratifying the analysis by partners' current pain, men whose partners had cancer and pain showed greater odds of psychological distress (odds ratio = 1.5, p = 0.04), compared with men whose partners had no cancer and had pain. However, male subjects whose partners had cancer but no pain did not show greater odds of psychological distress compared with men whose partners had no cancer and no pain. By contrast, in women whose partners had cancer, psychological distress was not associated with pain status. In conclusion, men whose partners had cancer and pain have higher risk of psychological distress, and its screening to these individuals may reduce the risk.

  8. Partner notification and partner treatment for chlamydia: attitude and practice of general practitioners in the Netherlands; a landscape analysis.

    Science.gov (United States)

    van den Broek, Ingrid V F; Donker, Gé A; Hek, Karin; van Bergen, Jan E A M; van Benthem, Birgit H B; Götz, Hannelore M

    2017-12-20

    Chlamydia prevalence remains high despite scaling-up control efforts. Transmission is not effectively interrupted without partner notification (PN) and (timely) partner treatment (PT). In the Netherlands, the follow-up of partners is not standardized and may depend on GPs' time and priorities. We investigated current practice and attitude of GPs towards PN and PT to determine the potential for Patient-Initiated Partner Treatment, which is legally not supported yet. Multiple data-sources were combined for a landscape analysis. Quantitative data on (potential) PT were obtained from prescriptions in the national pharmacy register (2004-2014) and electronic patient data from NIVEL-Primary Care Database (PCD) and from STI consultations in a subgroup of sentinel practices therein. Furthermore, we collected information on current practice via two short questionnaires at a national GP conference and obtained insight into GPs' attitudes towards PN/PT in a vignette study among GPs partaking in NIVEL-PCD. Prescription data showed Azithromycin double dosages in 1-2% of cases in the pharmacy register (37.000 per year); probable chlamydia-specific repeated prescriptions or double dosages of other antibiotics in NIVEL-PCD (115/1078) could not be interpreted as PT for chlamydia with certainty. STI consultation data revealed direct PT in 6/100 cases, via partner prescription or double doses. In the questionnaires the large majority of GPs (>95% of 1411) reported to discuss PN of current and ex-partner(s) with chlamydia patients. Direct PT was indicated as most common method by 4% of 271 GPs overall and by 12% for partners registered in the same practice. Usually, GPs leave further steps to the patients (83%), advising patients to tell partners to get tested (56%) or treated (28%). In the vignette study, 16-20% of 268 GPs indicated willingness to provide direct PT, depending on patient/partner profile, more (24-45%) if patients would have the chance to notify their partner first

  9. Identification of transient hub proteins and the possible structural basis for their multiple interactions.

    Science.gov (United States)

    Higurashi, Miho; Ishida, Takashi; Kinoshita, Kengo

    2008-01-01

    Proteins that can interact with multiple partners play central roles in the network of protein-protein interactions. They are called hub proteins, and recently it was suggested that an abundance of intrinsically disordered regions on their surfaces facilitates their binding to multiple partners. However, in those studies, the hub proteins were identified as proteins with multiple partners, regardless of whether the interactions were transient or permanent. As a result, a certain number of hub proteins are subunits of stable multi-subunit proteins, such as supramolecules. It is well known that stable complexes and transient complexes have different structural features, and thus the statistics based on the current definition of hub proteins will hide the true nature of hub proteins. Therefore, in this paper, we first describe a new approach to identify proteins with multiple partners dynamically, using the Protein Data Bank, and then we performed statistical analyses of the structural features of these proteins. We refer to the proteins as transient hub proteins or sociable proteins, to clarify the difference with hub proteins. As a result, we found that the main difference between sociable and nonsociable proteins is not the abundance of disordered regions, in contrast to the previous studies, but rather the structural flexibility of the entire protein. We also found greater predominance of charged and polar residues in sociable proteins than previously reported.

  10. Parents and partners: Moderating and mediating influences on intimate partner violence across adolescence and young adulthood.

    Science.gov (United States)

    Kaufman-Parks, Angela M; DeMaris, Alfred; Giordano, Peggy C; Manning, Wendy D; Longmore, Monica A

    2017-12-01

    Prior work examining intimate partner violence (IPV) among young adults often has emphasized familial characteristics, such as parent-child physical aggression (PCPA), and romantic relationship dynamics, such as jealousy and controlling behaviors, but has not considered these two domains simultaneously. Likewise, research examining how these two domains affect IPV perpetration over time for young adults is still limited. Using five waves of data from the Toledo Adolescent Relationships Study (N = 950), the present study examined the influence of parent-child relationship factors and romantic relationship dynamics in both their main and interactive effects on IPV perpetration spanning adolescence through young adulthood. Results from random-effects analyses indicated that both familial and romantic relationship dynamics should be taken into account when predicting IPV perpetration. Importantly, these two domains interacted to produce cumulatively different risk for engaging in violence against a romantic partner. Individuals were more likely to perpetrate IPV when their romantic relationship was characterized by verbal aggression if they reported PCPA experiences.

  11. Poxviral Ankyrin Proteins

    Directory of Open Access Journals (Sweden)

    Michael H. Herbert

    2015-02-01

    Full Text Available Multiple repeats of the ankyrin motif (ANK are ubiquitous throughout the kingdoms of life but are absent from most viruses. The main exception to this is the poxvirus family, and specifically the chordopoxviruses, with ANK repeat proteins present in all but three species from separate genera. The poxviral ANK repeat proteins belong to distinct orthologue groups spread over different species, and align well with the phylogeny of their genera. This distribution throughout the chordopoxviruses indicates these proteins were present in an ancestral vertebrate poxvirus, and have since undergone numerous duplication events. Most poxviral ANK repeat proteins contain an unusual topology of multiple ANK motifs starting at the N-terminus with a C-terminal poxviral homologue of the cellular F-box enabling interaction with the cellular SCF ubiquitin ligase complex. The subtle variations between ANK repeat proteins of individual poxviruses suggest an array of different substrates may be bound by these protein-protein interaction domains and, via the F-box, potentially directed to cellular ubiquitination pathways and possible degradation. Known interaction partners of several of these proteins indicate that the NF-κB coordinated anti-viral response is a key target, whilst some poxviral ANK repeat domains also have an F-box independent affect on viral host-range.

  12. Novel binding partners and differentially regulated phosphorylation sites clarify Eps8 as a multi-functional adaptor.

    Directory of Open Access Journals (Sweden)

    Debbie L Cunningham

    Full Text Available Eps8 is involved in both cell signalling and receptor trafficking. It is a known phosphorylation substrate for two proteins involved in the fibroblast growth factor receptor (FGFR signalling pathway: the receptor itself and Src. Here we report a differential proteomic analysis of Eps8 aimed to identify specific FGFR and Src family kinase dependent phosphosites and co-associated phosphodependent binding partners. This study reveals a total of 22 Eps8 pTyr and pSer/Thr phosphorylation sites, including those that are dependent on Src family and FGFR kinase activity. Peptide affinity purification of proteins that bind to a selection of the pTyr phosphosites has identified a range of novel Eps8 binding partners including members of the intracellular vesicle trafficking machinery (clathrin and AP-2, proteins which have been shown to regulate activated receptor trafficking (NBR1 and Vav2, and proteins involved in receptor signalling (IRS4 and Shp2. Collectively this study significantly extends the understanding of Eps8 post-translational modification by regulated phosphorylation, identifies novel Eps8 binding partners implicated in receptor trafficking and signalling, and confirms the functions of Eps8 at the nexus of receptor signalling and vesicular trafficking.

  13. Spinal-Cord-Injured Individual's Experiences of Having a Partner

    DEFF Research Database (Denmark)

    Angel, Sanne

    2015-01-01

    Having a partner is a strong factor in adaptation to the new life situation with a spinal cord injury (SCI). Still, more knowledge in detail about the partner's influences according to the experiences of individuals with SCI could contribute to the understanding of the situation after an injury. ...... and allowed SCI individuals the ability to self-realize. This promoted feelings of profound gratitude but also dependency. Thus, the SCI individual benefitted from the partner's support mentally and physically, which enabled a life that would not otherwise be possible....

  14. Partnering Strategies for Childhood Agricultural Safety and Health

    Science.gov (United States)

    Hard, David L.

    2015-01-01

    The National Institute for Occupational Safety and Health (NIOSH) has been the lead federal agency of the national Childhood Agricultural Injury Prevention Initiative (CAIPI) since the program's inception in 1996 and in this role, collaborated with numerous partners in childhood agricultural injury prevention activities. This collaboration has likely helped achieve the current reduction in childhood agricultural injury. The paper looks at existing groups with past and current childhood agricultural injury prevention activities for partnering strategies that could contribute to reducing the morbidity and mortality of childhood agricultural injuries. Based upon the review, suggestions are made for future partnering strategies to continue progress in this area. PMID:22490034

  15. Intimate partner violence: psychological and verbal abuse during pregnancy.

    Science.gov (United States)

    Debono, Christie; Borg Xuereb, Rita; Scerri, Josianne; Camilleri, Liberato

    2017-08-01

    To examine the association between sociodemographic, pregnancy-related variables and psychological and verbal intimate partner abuse, as well as to determine which of these variables are predictors of psychological and verbal intimate partner abuse during pregnancy. Intimate partner violence is a significant health issue, with severe implications to both mother and foetus. However, much of the research to date focuses on the outcomes of physical abuse. This article addresses the dearth in the literature by examining the association between sociodemographic, pregnancy-related variables and psychological and verbal intimate partner abuse during pregnancy. A survey research design was used. Three hundred postnatal women were recruited by convenience, nonproportional quota sampling technique. The WHO Violence Against Women Instrument was self-administered by participants. The association between categorical variables was assessed using Pearson's chi-square test, the strength of association using Cramer's V and the phi coefficient, and the identification of predictor variables for psychological and verbal abuse using logistic regression analysis. Four predictors were identified for psychological abuse, namely low education level in women, an unplanned pregnancy, experiencing two or more pregnancy-related health problems and living with an unemployed partner. However, unemployment in women, an unplanned pregnancy, fear of partner and a low education level of partner were identified as the predictors of verbal abuse. This study identified a number of variables that strongly predict psychological and verbal intimate partner abuse during pregnancy; however, it extends the available literature by identifying a low standard of education in males, unemployment and fear of the intimate partner as the significant predictors of psychological and verbal intimate partner abuse. Healthcare professionals should be aware of the predictors predisposing pregnant women to abuse. This

  16. Partner notification for sexually transmitted infections and perception of notified partners.

    Science.gov (United States)

    Cavalcante, Elani Graça Ferreira; Miranda, Mahara Coelho Crisostomo; Carvalho, Ana Zaiz Flores Hormain Teixeira de; Lima, Ivana Cristina Vieira de; Galvão, Marli Teresinha Gimeniz

    2016-01-01

    Learn the perceptions of patients with sexually transmitted infections and sexual partners who are notified of the infection. A descriptive and qualitative study, based on the collective subject discourse technique, was conducted in four healthcare centers of reference in Fortaleza, Ceará, from March to July 2014. The sample comprised 21 subjects (11 index patients and 10 notified partners). The index patients reported complicity, concern about the partner's health and revelation of diagnosis aiming to preserve the relationship. The partners showed antagonistic perceptions: tranquility-betrayal, fear of death, of incurability and the diagnosis, especially of HIV. The reasons for coming to a healthcare center were: fear of being sick, attenuation of guilt of infection transmission, need for diagnosis, early start of treatment. Fear of losing trust, insecurities when dealing with a sexual infection and being responsible or co-responsible for the transmission were the predominant feelings. Various types of partner notification were reported (verbal, telephone, notification card), according to individual convenience. This study suggests the use of alternative methods of notification and an integrated system of notification. Conhecer as percepções dos pacientes com infecções sexualmente transmissíveis e parceiros sexuais sobre a notificação da infecção. Estudo descritivo e qualitativo, baseado na técnica do discurso do sujeito coletivo, realizado em quatro Unidades de Saúde de referência em Fortaleza/CE, de março a julho de 2014. Amostra composta por 21 sujeitos (11 pacientes-índice e 10 parceiros notificados). Pacientes-índice relataram cumplicidade, preocupação com a saúde do parceiro e revelação do diagnóstico como forma de preservação do relacionamento. Para os parceiros, as percepções foram antagônicas: tranquilidade-traição, medo da morte, da incurabilidade e do diagnóstico, especialmente do HIV. Os motivos para o comparecimento foram

  17. Doc of prophage P1 is inhibited by its antitoxin partner Phd through fold complementation

    DEFF Research Database (Denmark)

    Garcia-Pino, Abel; Christensen-Dalsgaard, Mikkel; Wyns, Lode

    2008-01-01

    Prokaryotic toxin-antitoxin modules are involved in major physiological events set in motion under stress conditions. The toxin Doc (death on curing) from the phd/doc module on phage P1 hosts the C-terminal domain of its antitoxin partner Phd (prevents host death) through fold complementation...... evolutionary origin for the phd/doc operon. Doc induces growth arrest of Escherichia coli cells in a reversible manner, by targeting the protein synthesis machinery. Moreover, Doc activates the endogenous E. coli RelE mRNA interferase but does not require this or any other known chromosomal toxin...

  18. Protein Foods

    Science.gov (United States)

    ... Text Size: A A A Listen En Español Protein Foods Foods high in protein such as fish, ... for the vegetarian proteins, whether they have carbohydrate. Protein Choices Plant-Based Proteins Plant-based protein foods ...

  19. Preparing Scientists to be Community Partners

    Science.gov (United States)

    Pandya, R. E.

    2012-12-01

    Many students, especially students from historically under-represented communities, leave science majors or avoid choosing them because scientific careers do not offer enough opportunity to contribute to their communities. Citizen science, or public participation in scientific research, may address these challenges. At its most collaborative, it means inviting communities to partner in every step of the scientific process from defining the research question to applying the results to community priorities. In addition to attracting and retaining students, this level of community engagement will help diversify science, ensure the use and usability of our science, help buttress public support of science, and encourage the application of scientific results to policy. It also offers opportunities to tackle scientific questions that can't be accomplished in other way and it is demonstrably effective at helping people learn scientific concepts and methods. In order to learn how to prepare scientists for this kind of intensive community collaboration, we examined several case studies, including a project on disease and public health in Africa and the professionally evaluated experience of two summer interns in Southern Louisiana. In these and other cases, we learned that scientific expertise in a discipline has to be accompanied by a reservoir of humility and respect for other ways of knowing, the ability to work collaboratively with a broad range of disciplines and people, patience and enough career stability to allow that patience, and a willingness to adapt research to a broader set of scientific and non-scientific priorities. To help students achieve this, we found that direct instruction in participatory methods, mentoring by community members and scientists with participatory experience, in-depth training on scientific ethics and communication, explicit articulation of the goal of working with communities, and ample opportunity for personal reflection were essential

  20. Intimate partner violence among rural South African men: alcohol use, sexual decision-making, and partner communication.

    Science.gov (United States)

    Hatcher, Abigail M; Colvin, Christopher J; Ndlovu, Nkuli; Dworkin, Shari L

    2014-01-01

    Nearly one-third of South African men report enacting intimate partner violence. Beyond the direct health consequences for women, intimate partner violence is also linked to varied risk behaviours among men who enact it, including alcohol abuse, risky sex, and poor healthcare uptake. Little is known about how to reduce violence perpetration among men. We conducted retrospective, in-depth interviews with men (n = 53) who participated in a rural South African programme that targeted masculinities, HIV risk, and intimate partner violence. We conducted computer-assisted thematic qualitative coding alongside a simple rubric to understand how the programme may lead to changes in men's use of intimate partner violence. Many men described new patterns of reduced alcohol intake and improved partner communication, allowing them to respond in ways that did not lead to the escalation of violence. Sexual decision-making changed via reduced sexual entitlement and increased mutuality about whether to have sex. Men articulated the intertwined nature of each of these topics, suggesting that a syndemic lens may be useful for understanding intimate partner violence. These data suggest that alcohol and sexual relationship skills may be useful levers for future violence prevention efforts, and that intimate partner violence may be a tractable issue as men learn new skills for enacting masculinities in their household and in intimate relationships.

  1. The emergence of partnering in construction practice: an activity theory perspective

    NARCIS (Netherlands)

    Hartmann, Andreas; Bresnen, Mike

    2011-01-01

    Over the last decades, interest has increased enormously in collaborative arrangements subsumed under the term partnering. Recent critics have stated that prescriptive approaches dominate the discussion on partnering in construction. There is a lack of multiple perspectives on the partnering

  2. Mapping Protein-Protein Interactions by Quantitative Proteomics

    DEFF Research Database (Denmark)

    Dengjel, Joern; Kratchmarova, Irina; Blagoev, Blagoy

    2010-01-01

    Proteins exert their function inside a cell generally in multiprotein complexes. These complexes are highly dynamic structures changing their composition over time and cell state. The same protein may thereby fulfill different functions depending on its binding partners. Quantitative mass...... spectrometry (MS)-based proteomics in combination with affinity purification protocols has become the method of choice to map and track the dynamic changes in protein-protein interactions, including the ones occurring during cellular signaling events. Different quantitative MS strategies have been used...... to characterize protein interaction networks. In this chapter we describe in detail the use of stable isotope labeling by amino acids in cell culture (SILAC) for the quantitative analysis of stimulus-dependent dynamic protein interactions....

  3. PARTNER project: Trained to battle cancer with particles

    CERN Multimedia

    2009-01-01

    The four CERN students participating in the PARTNER project. From left to right: Daniel Abler (Germany), Faustin Laurentiu Roman (Romania), Vassiliki Kanellopoulos (Greece/Germany) and Till Tobias Boehlen (Germany).

  4. Everyday partner violence in Rwanda: The contribution of ...

    African Journals Online (AJOL)

    -based sociotherapy as a grassroots intervention has proven to be an effective complement to more top-down public and political responses to gender-based violence. Keywords: partner violence, family conflict, community-based sociotherapy, ...

  5. Public-Private Partnerships: NASA as Your Business Partner

    Science.gov (United States)

    Martin, Gary

    2017-01-01

    Partnerships is an important part of doing business at NASA. NASA partners with external organizations to access capabilities under collaborative agreements; enters into agreements for partner access to NASA capabilities; expand overall landscape of space activity; and spurring innovation. The U.S. national policy on commercial space is to develop a robust and competitive U.S. commercial space sector and to energize competitive domestic industries to participate in global markets. Commercial space must be competitive, while the government has other priorities such as safety, jobs, etc. NASA partnerships consist of Reimbursable and Non-Reimbursable Space Act Agreements. Partnerships at Ames aligns with Ames' core competencies, and Partners often office in the NASA Research Park, which is an established regional innovation cluster that facilitates commercialization and services as a technology accelerator via onsite collaborations between NASA and its partners.

  6. Peer tutors as learning and teaching partners: a cumulative ...

    African Journals Online (AJOL)

    Peer tutors as learning and teaching partners: a cumulative approach to building peer tutoring capacity in higher education. ... when training and development is theoretically informed, coherent, and oriented towards improving practice. Keywords: academic development, academic literacies, cumulative learning, higher

  7. Intimate partner violence in early adolescence: The role of gender ...

    African Journals Online (AJOL)

    Intimate partner violence in early adolescence: The role of gender, socioeconomic factors and the school. AJ Mason-Jones, P De Koker, SM Eggers, C Mathews, M Temmerman, E Leye, PJ de Vries, H de Vries ...

  8. Partner Support for Family Planning and Modern Contraceptive Use ...

    African Journals Online (AJOL)

    USER

    partner's approval. Difference between the meaning of approval and encouragement should be explored. Interventions involving information education and communication campaigns geared to men and promoting male involvement in family ...

  9. Intimate partner violence among HIV infected and uninfected ...

    African Journals Online (AJOL)

    Intimate partner violence among HIV infected and uninfected pregnant women delivering at a National Hospital in Tanzania: using a modified screening tool. Hussein L. Kidanto, Andrew H. Mgaya, Birgitta Essen ...

  10. Gay men and intimate partner violence: a gender analysis

    National Research Council Canada - National Science Library

    Oliffe, John L; Han, Christina; Maria, Estephanie Sta; Lohan, Maria; Howard, Terry; Stewart, Donna E; MacMillan, Harriet

    2014-01-01

    Though intimate partner violence ( IPV ) is predominately understood as a women's health issue most often emerging within heterosexual relationships, there is increasing recognition of the existence of male victims of IPV...

  11. PARTNERING TO COLLECT IMPROVED HUMAN EXPOSURE MEASUREMENT DATA

    Science.gov (United States)

    Partnering allows complex environmental challenges to be efficiently addressed by leveraging resources and scientific expertise. The US Environmental Protection Agency's Office of Research and Development (ORD) routinely collaborates with other federal, state, and local governme...

  12. States and ORD: Partners to Meet State Research Needs

    Science.gov (United States)

    EPA Reseach staff holds Tools Cafes for specific Agency partners and stakeholders. At these Tools Cafes, we share and demonstrate tools, talk with users and potential users, and solicit feedback to continually improve and update our resources.

  13. Tackling gender inequalities and intimate partner violence in the ...

    African Journals Online (AJOL)

    : moving towards effective interventions in Southern and Eastern Africa. ... African Journal of AIDS Research ... Ending intimate partner violence (IPV) and reducing gender inequalities are recognised as critical to “'ending AIDS” by 2030.

  14. Trust and partner-enhancing attributions in close relationships.

    Science.gov (United States)

    Miller, Paul J E; Rempel, John K

    2004-06-01

    A cross-lagged panel design was used to examine the links between trust and attributional processes in a sample of 75 married couples throughout a period of 2 years. During the first phase of the study, participants completed a measure of marital trust, engaged in a laboratory problem-solving discussion of a recurrent conflict-related issue, and then rated their partner's behavior and motives. Approximately 2 years later, 54 couples were again contacted and measures of trust were obtained. Forty of these couples also viewed a videotape of their laboratory problem-solving discussion from 2 years previously and rated their partner's behavior and motives. Results suggested a reciprocal causal pattern by which partner-enhancing attributions predict changes in trust and trust predicts changes in partner-enhancing attributions.

  15. Partner selection and governance design in interfirm relationships

    NARCIS (Netherlands)

    Dekker, H.C.

    2008-01-01

    This paper develops a structural model to simultaneously analyze firms' partner selection activities and governance design for managing interfirm relationships. The model predicts these choices to be influenced by appropriation concerns, coordination requirements, dependence and prior experiences

  16. China's Investments in Malaysia: Choosing the "Right" Partners

    National Research Council Canada - National Science Library

    Guanie Lim

    2015-01-01

      This paper examines China's outward foreign direct investment into Malaysia by analysing the major coalition partners of mainland Chinese firms that have invested into the country, a relatively underexplored topic...

  17. Residence in Switzerland of partners of members of the personnel

    CERN Multimedia

    2012-01-01

    1. Definitions a) CERN Staff Rules and Regulations Article S IV 1.02 of the Staff Rules defines a "partner", irrespective of gender, as "any person linked to an employed member of the personnel by a partnership officially registered in a Member State". Partners are regarded as family members for the purposes of protection against the financial consequences of illness and accidents. b) Swiss Federal Law Under Swiss federal law, to which the text below essentially refers, the following definitions apply: "partners": a couple of the same sex (linked by a registered partnership), "common-law spouses": a couple of the opposite sex (unmarried). Provided that they are aged 18 or more and are not blood relatives, two people of the same sex ("partners") may officially register their partnership with the competent registry office in order to give it a legal framework (a civil partnership commonly known as the Federal PACS...

  18. Servitization in China via an External Service Partner Network

    DEFF Research Database (Denmark)

    Raja, Jawwad; Frandsen, Thomas

    2016-01-01

    Previous research has predominately focused on the servitization strategies of Western manufacturers in advanced economies, neglecting the potential in those which are emerging, such as China. This paper explores the role of the external service partner network of a European manufacturer providing...... services in China in order to develop a better understanding of the challenges. An in-depth multiple case study approach was taken to examine the parent company, its subsidiary in China and the related service partner network. Data collection involved all three actors and took place in Denmark and China....... The findings suggest that motivation, opportunity and ability (MOA) need not only be mutually reinforcing for the organization attempting to move towards services but also congruent within the external service partner network. Furthermore, the findings suggest that an external service partner may not be able...

  19. Perceived medical benefit, peer/partner influence and safety and ...

    African Journals Online (AJOL)

    Perceived medical benefit, peer/partner influence and safety and cost to access the service: client motivators for voluntary seeking of medical male circumcision in Iganga District Eastern Uganda, a qualitative study.

  20. Expedited partner treatment for sexually transmitted infections: an update.

    Science.gov (United States)

    Kissinger, Patricia; Hogben, Matthew

    2011-04-01

    To date, seven randomized trials have evaluated the efficacy of expedited partner treatment (EPT). These trials have included heterosexual men and women and examine EPT for Chlamydia trachomatis, Neisseria gonorrhoea, and Trichomonas vaginalis. These studies demonstrated either superiority for percentage of partners being treated, for a reduction in repeat infections, or cost benefit for EPT compared to the standard partner referral method and reported no adverse events. In the United States, although the number of states where EPT is legal continues to grow, adoption of EPT remains low. Provider concerns about liability and payment issues continue to be a barrier to implementation of EPT. More translational research is needed to improve adoption by the players involved: index patients, partners, providers, and payers.

  1. Pamplin College of Business partners with CFA Institute

    OpenAIRE

    Ho, Sookhan

    2007-01-01

    Virginia Tech's Pamplin College of Business has been named a CFA Program Partner of CFA Institute, the global, non-profit professional association that administers the Chartered Financial Analyst (CFA) curriculum and examination program.

  2. The global prevalence of intimate partner homicide: a systematic review.

    Science.gov (United States)

    Stöckl, Heidi; Devries, Karen; Rotstein, Alexandra; Abrahams, Naeemah; Campbell, Jacquelyn; Watts, Charlotte; Moreno, Claudia Garcia

    2013-09-07

    Homicide is an important cause of premature mortality globally, but evidence for the magnitude of homicides by intimate partners is scarce and hampered by the large amount of missing information about the victim-offender relationship. The objective of the study was to estimate global and regional prevalence of intimate partner homicide. A systematic search of five databases (Medline, Global Health, Embase, Social Policy, and Web of Science) yielded 2167 abstracts, and resulted in the inclusion of 118 full-text articles with 1122 estimates of the prevalence of intimate partner homicide after double-blind screening. All studies were included that reported the number or proportion of women or men who were murdered by an intimate partner in a country, province, or town, using an inclusive definition of an intimate partner. Additionally, a survey of official sources of 169 countries provided a further 53 estimates. We selected one estimate per country-year using a quality assessment decision algorithm. The median prevalence of intimate partner homicide was calculated by country and region overall, and for women and men separately. Data were obtained for 66 countries. Overall 13·5% (IQR 9·2-18·2) of homicides were committed by an intimate partner, and this proportion was six times higher for female homicides than for male homicides (38·6%, 30·8-45·3, vs 6·3%, 3·1-6·3). Median percentages for all (male and female) and female intimate partner homicide were highest in high-income countries (all, 14·9%, 9·2-18·2; female homicide, 41·2%, 30·8-44·5) and in southeast Asia (18·8%, 11·3-18·8; 58·8%, 58·8-58·8). Adjustments to account for unknown victim-offender relationships generally increased the prevalence, suggesting that results presented are conservative. At least one in seven homicides globally and more than a third of female homicides are perpetrated by an intimate partner. Such violence commonly represents the culmination of a long history of abuse

  3. Intimate partner violence among male and female students in Kosovo

    OpenAIRE

    Kelmendi, K.; Baumgartner, F.

    2014-01-01

    The aim of this paper was to analyze the rates of intimate partner violence focusing on physical, psychological and sexual violence using the Conflict Tactics Scale (CTS-2) using the sample of 700 students from main public university in Pristina. Findings from the study show high rates of perpetration of intimate partner violence; physical violence (42.1%), psychological violence (64.7%) and sexual violence (34.6%), including both minor and severe acts, however, the majority of acts were mino...

  4. Antecedents of Intimate Partner Violence Among Gay and Bisexual Men

    OpenAIRE

    Finneran, Catherine; Stephenson, Rob

    2014-01-01

    Examinations of gay and bisexual men’s (GBM) perceptions of intimate partner violence (IPV), including their perceptions of events likely to precipitate IPV, are lacking. Focus group discussions with GBM (n = 83) yielded 24 unique antecedents, or triggers, of IPV in male–male relationships. Venue-recruited survey participants (n = 700) identified antecedents that were likely to cause partner violence in male–male relationships, including antecedents GBM-specific currently absent from the lite...

  5. Strength at Home Couples Program to Prevent Military Partner Violence

    Science.gov (United States)

    2017-10-01

    AWARD NUMBER: W81XWH-15-1-0374 TITLE: Strength at Home Couples Program to Prevent Military Partner Violence PRINCIPAL INVESTIGATOR: Casey T...SUBTITLE 5a. CONTRACT NUMBER Strength at Home Couples Program to Prevent Military Partner Violence 5b. GRANT NUMBER W81XWH-15-1-0374 5c. PROGRAM...Health 16. SECURITY CLASSIFICATION OF: 17. LIMITATION OF ABSTRACT 18. NUMBER OF PAGES 19a. NAME OF RESPONSIBLE PERSON USAMRMC a. REPORT U b

  6. Making Sense of Partnering: Discourses, Governance and Institutional Change

    DEFF Research Database (Denmark)

    Gottlieb, Stefan Christoffer; Jensen, Jens Stissing

    2012-01-01

    Coordination in construction projects has traditionally been based on contractually defined relations involving high degrees of surveillance. In recent decades, partnering has been advocated as a project-specific, communicative alternative to this contractual mode of project governance. Taking....... Thus, as a project governance mechanism, partnering emerges as a collective sense-making process directed at (re-)creating a new form of rational behaviour under changing institutional conditions....

  7. Virginia Tech launches corporate partners program in biological sciences

    OpenAIRE

    Doss, Catherine

    2007-01-01

    Virginia Tech's Department of Biological Sciences in the university's College of Science, has launched a corporate partners program to foster collaboration between faculty, students and bio-science oriented corporations in the mid-Atlantic region. The so-named Biological Sciences Partners in Research and Education (BioSPIRE) program is designed to engage companies with an interest and capacity to impact education in the biological sciences.

  8. Using smartphone apps in STD interviews to find sexual partners.

    Science.gov (United States)

    Pennise, Melissa; Inscho, Roxana; Herpin, Kate; Owens, John; Bedard, Brenden A; Weimer, Anita C; Kennedy, Byron S; Younge, Mary

    2015-01-01

    Smartphone applications (apps) are increasingly used to facilitate casual sexual relationships, increasing the risk of sexually transmitted diseases (STDs). In STD investigations, traditional contact elicitation methods can be enhanced with smartphone technology during field interviews. In 2013, the Monroe County Department of Public Health conducted a large, multi-infection STD investigation among men who have sex with men (MSM) using both index case and cluster interviews. When patients indicated meeting sexual partners online, disease intervention specialists (DISs) had access to smartphone apps and were able to elicit partners through access to inboxes and profiles where traditional contact information was lacking. Social network mapping was used to display the extent of the investigation and the impact of access to smartphones on the investigation. A total of 14 index patient interviews and two cluster interviews were conducted; 97 individuals were identified among 117 sexual dyads. On average, eight partners were elicited per interview (range: 1-31). The seven individuals who used apps to find partners had an average of three Internet partners (range: 1-5). Thirty-six individuals either had a new STD (n=7) or were previously known to be HIV-positive (n=29). Of the 117 sexual dyads, 21 (18%) originated either online (n=8) or with a smartphone app (n=13). Of those originating online or with a smartphone app, six (29%) partners were located using the smartphone and two (10%) were notified of their exposure via a website. Three of the new STD/HIV cases were among partners who met online. Smartphone technology used by DISs in the field improved contact elicitation and resulted in successful partner notification and case finding.

  9. The EU funded PARTNER project for ICTR-PHE 2012

    CERN Multimedia

    Greco, V

    2012-01-01

    PARTNER is a 4-­‐‑year Marie Curie Training project funded by the European Commission that aimed at the creation of the next generation of experts in hadron-­‐‑therapy. Ten academic institutes and research centres and two leading companies (Siemens and IBA) are participating in PARTNER, which is coordinated by CERN, forming a unique multidisciplinary and multinational European network.

  10. Communication Partner Training in Aphasia: An Updated Systematic Review.

    Science.gov (United States)

    Simmons-Mackie, Nina; Raymer, Anastasia; Cherney, Leora R

    2016-12-01

    To update a previous systematic review describing the effect of communication partner training on individuals with aphasia and their communication partners, with clinical questions addressing effects of partner training on language, communication activity/participation, psychosocial adjustment, and quality of life. Twelve electronic databases were searched using 23 search terms. References from relevant articles were hand searched. Three reviewers independently reviewed abstracts, excluding those that failed to meet inclusion criteria. Thirty-two full text articles were reviewed by 2 independent reviewers. Articles not meeting inclusion criteria were eliminated, resulting in a corpus of 25 articles for full review. For the 25 articles, 1 reviewer extracted descriptive data regarding participants, intervention, outcome measures, and results. A second reviewer verified the accuracy of the extracted data. The 3-member review team classified studies using the American Academy of Neurology levels of evidence. Two independent reviewers evaluated each article using design-specific tools to assess research quality. All 25 of the current review articles reported positive changes from partner training. Therefore, to date, 56 studies across 2 systematic reviews have reported positive outcomes from communication partner training in aphasia. The results of the current review are consistent with the previous review and necessitate no change to the earlier recommendations, suggesting that communication partner training should be conducted to improve partner skill in facilitating the communication of people with chronic aphasia. Additional high-quality research is needed to strengthen the original 2010 recommendations and expand recommendations to individuals with acute aphasia. High-quality clinical trials are also needed to demonstrate implementation of communication partner training in complex environments (eg, health care). Copyright © 2016 American Congress of

  11. Consistency and inconsistency among romantic partners over time.

    Science.gov (United States)

    Eastwick, Paul W; Harden, K Paige; Shukusky, Jennifer A; Morgan, Taylor Anne; Joel, Samantha

    2017-06-01

    Theoretical perspectives on mating differentially emphasize whether (and why) romantic partner selection and maintenance processes derive from stable features of individuals (e.g., mate value, mate preferences, relationship aptitude) and their environments (e.g., social homogamy) rather than adventitious, dyad-specific, or unpredictable factors. The current article advances our understanding of this issue by assessing how people's actual romantic partners vary on constructs commonly assessed in evolutionary psychology (Study 1), sociology (Study 2), and close relationships (Study 3). Specifically, we calculated the extent to which the past and present partners of a focal person (i.e., the person who dated all of the partners) cluster on various measures. Study 1 investigated consistency in the observable qualities of the romantic partners, revealing substantial evidence for clustering on coder-rated attributes like attractiveness and masculinity. Study 2 examined qualities self-reported by romantic partners themselves in a demographically diverse sample and found modest evidence for clustering on attributes such as IQ and educational aspirations; however, clustering in this study was largely due to demographic stratification. Study 3 explored target-specific ratings by partners about the focal person and found little evidence for clustering: The ability to elicit high romantic desirability/sexual satisfaction ratings from partners was not a stable individual difference. The variables that affect mating may differ considerably in the extent to which they serve as stable versus unpredictable factors; thus, the fields of evolutionary psychology, sociology, and close relationships may reveal distinct depictions of mating because the constructs and assessment strategies in each differ along this underappreciated dimension. (PsycINFO Database Record (c) 2017 APA, all rights reserved).

  12. Discovery of novel interacting partners of PSMD9, a proteasomal chaperone: Role of an Atypical and versatile PDZ-domain motif interaction and identification of putative functional modules.

    Science.gov (United States)

    Sangith, Nikhil; Srinivasaraghavan, Kannan; Sahu, Indrajit; Desai, Ankita; Medipally, Spandana; Somavarappu, Arun Kumar; Verma, Chandra; Venkatraman, Prasanna

    2014-01-01

    PSMD9 (Proteasome Macropain non-ATPase subunit 9), a proteasomal assembly chaperone, harbors an uncharacterized PDZ-like domain. Here we report the identification of five novel interacting partners of PSMD9 and provide the first glimpse at the structure of the PDZ-domain, including the molecular details of the interaction. We based our strategy on two propositions: (a) proteins with conserved C-termini may share common functions and (b) PDZ domains interact with C-terminal residues of proteins. Screening of C-terminal peptides followed by interactions using full-length recombinant proteins, we discovered hnRNPA1 (an RNA binding protein), S14 (a ribosomal protein), CSH1 (a growth hormone), E12 (a transcription factor) and IL6 receptor as novel PSMD9-interacting partners. Through multiple techniques and structural insights, we clearly demonstrate for the first time that human PDZ domain interacts with the predicted Short Linear Sequence Motif (SLIM) at the C-termini of the client proteins. These interactions are also recapitulated in mammalian cells. Together, these results are suggestive of the role of PSMD9 in transcriptional regulation, mRNA processing and editing, hormone and receptor activity and protein translation. Our proof-of-principle experiments endorse a novel and quick method for the identification of putative interacting partners of similar PDZ-domain proteins from the proteome and for discovering novel functions.

  13. HomPPI: a class of sequence homology based protein-protein interface prediction methods

    Directory of Open Access Journals (Sweden)

    Dobbs Drena

    2011-06-01

    Full Text Available Abstract Background Although homology-based methods are among the most widely used methods for predicting the structure and function of proteins, the question as to whether interface sequence conservation can be effectively exploited in predicting protein-protein interfaces has been a subject of debate. Results We studied more than 300,000 pair-wise alignments of protein sequences from structurally characterized protein complexes, including both obligate and transient complexes. We identified sequence similarity criteria required for accurate homology-based inference of interface residues in a query protein sequence. Based on these analyses, we developed HomPPI, a class of sequence homology-based methods for predicting protein-protein interface residues. We present two variants of HomPPI: (i NPS-HomPPI (Non partner-specific HomPPI, which can be used to predict interface residues of a query protein in the absence of knowledge of the interaction partner; and (ii PS-HomPPI (Partner-specific HomPPI, which can be used to predict the interface residues of a query protein with a specific target protein. Our experiments on a benchmark dataset of obligate homodimeric complexes show that NPS-HomPPI can reliably predict protein-protein interface residues in a given protein, with an average correlation coefficient (CC of 0.76, sensitivity of 0.83, and specificity of 0.78, when sequence homologs of the query protein can be reliably identified. NPS-HomPPI also reliably predicts the interface residues of intrinsically disordered proteins. Our experiments suggest that NPS-HomPPI is competitive with several state-of-the-art interface prediction servers including those that exploit the structure of the query proteins. The partner-specific classifier, PS-HomPPI can, on a large dataset of transient complexes, predict the interface residues of a query protein with a specific target, with a CC of 0.65, sensitivity of 0.69, and specificity of 0.70, when homologs of

  14. A screen for potential ferredoxin electron transfer partners uncovers new, redox dependent interactions.

    Science.gov (United States)

    Hanke, G T; Satomi, Y; Shinmura, K; Takao, T; Hase, T

    2011-02-01

    Ferredoxin (Fd) is the primary soluble acceptor at the end of the photosynthetic electron transport chain, and is known to directly transfer electrons to a wide range of proteins for use in metabolism and regulatory processes. We have conducted a screen to identify new putative Fd interaction partners in the cyanobacteria Synechocystis sp. PCC 6803 using Fd-chromatography in combination with MALDI-TOF mass spectrometry. Many novel interactions were detected, including several redox enzymes, which are now candidates for further experiments to investigate electron transfer with Fd. In addition, some proteins with regulatory activity related to photosynthesis were identified. We cloned and expressed one such protein, known as RpaA, which is a specific regulator of energy transfer between phycobilisomes and PSI. Using the recombinant protein we confirmed direct interaction with Fd, and discovered that this was dependent on redox state. The screen for putative Fd-binding proteins was repeated, comparing oxidizing and reducing conditions, identifying many proteins whose interaction with Fd is redox dependent. These include several additional signaling molecules, among them the LexA repressor, Ycf53 and NII, which are all involved in interpreting the redox state of the cell. Copyright © 2010 Elsevier B.V. All rights reserved.

  15. THE INTIMATE PARTNER VIOLENCE RESPONSIBILITY ATTRIBUTION SCALE (IPVRAS

    Directory of Open Access Journals (Sweden)

    Marisol Lila

    2014-01-01

    Full Text Available The aim of this study is to present a psychometrically sound instrument to assess intimate partner violence offenders’ responsibility attributions: the Intimate Partner Violence Responsibility Attribution Scale. The scale was administrated to 423 adult male intimate partner violence offenders court-mandated to a community-based intervention program. A three factor structure (responsibility attribution to the legal system, responsibility attribution to the victim, and responsibility attribution to the offender personal context was supported using confirmatory factor analysis. Reliability of the scales in this study was estimated using Cronbach’s alpha, ρ and greatest lower bound. The Intimate Partner Violence Responsibility Attribution Scale correlated in theoretically expected ways with variables used to assess construct validity (system blaming, problems with partner, and responsibility assumption and with variables used to assess criterion-related validity (satisfaction with legal system, victim-blaming attitudes, alcohol consumption, hostile sexism, stressful life events, social desirability, impulsivity and household income. Results support the validity and reliability of the Intimate Partner Violence Responsibility Attribution Scale

  16. Intimate partner violence and unintended pregnancy: prevalence and associated factors

    Directory of Open Access Journals (Sweden)

    Ana Carolina da C. Azevêdo

    2013-12-01

    Full Text Available This study investigated the association between unintended pregnancy and intimate partner violence before pregnancy. A cross-sectional study was carried out with 1,054 women, aged 18 to 49, in Recife, Northeastern Brazil, from July 2005 to March 2006. Non-conditional logistic regression analysis was performed with a hierarchical strategy for entering variables into the model, according to the conceptual framework defined. Unintended pregnancy was reported by 60.3% (636 women. Intimate partner violence prior to the pregnancy was associated with unintended pregnancy (ORadj = 1.57; 95%CI: 1.17-2.11, even when adjusted for the women's sociodemographic characteristics, the partner's behaviour, and the relationship dynamic. When the association was adjusted for the use of contraception and the partner's refusal to use contraception, the association was no longer significant, suggesting that the effect of partner violence on unintended pregnancy may be mediated by these variables. The findings point to the need of screening for intimate partner violence in reproductive health services.

  17. Are romantic partners a source of college students' weight concern?

    Science.gov (United States)

    Sheets, Virgil; Ajmere, Kavita

    2005-01-01

    This study explored the importance of weight in college students' dating relationships and the expression of weight-related concerns between dating romantic partners. In a survey of 554 undergraduates, we found that overweight women were less likely to be dating than their peers, and that weight was positively correlated with relationship satisfaction in men, but negatively correlated with satisfaction in women. We also found that over 30% of students in exclusive relationships had either been told by their partner (or had told their partner) to gain or lose weight. While male partners were typically told to gain weight, female partners were as likely to be told to lose as gain weight. Women's weight-loss attempts and self-esteem were not related to being the targets of these comments, but women told to lose weight reported lower relationship satisfaction; men's self-esteem was not related to being the targets of these comments, but men told to gain weight reported lower relationship satisfaction. Although our results support prior research suggesting that weight is differentially important for men and women in romantic relationships, they challenge the hypothesis that individual romantic partners are a primary source of students' body dissatisfaction and weight concerns.

  18. Hepatitis C treatment - better outcomes through partner support

    Directory of Open Access Journals (Sweden)

    Fleur Chapman

    2012-11-01

    Full Text Available AbstractBackground Globally, it is estimated that 170 million people are living with hepatitis C and between three and four million are newly infected annually. In Australia, around 1% of people are living with chronic hepatitis C, with two-thirds of these being men. Aims This research aimed to determine the impact of hepatitis C treatment on partners of patients using in-depth exploratory techniques. Method Four infected men and their partners (n= 8 participants and three service providers were recruited and interviewed separately to identify the needs of female partners supporting patients with Hepatitis C. Discussion was based on the experiences of female partners during the treatment phase of male hepatitis C patients. Results All participants recognised a need for greater assistance for partners of hepatitis C treatment patients. It was also recognised that strong social support improved treatment outcomes and helped to maintain the survival of family relationships during the intensive treatment phase. Conclusion Although this research was limited by size, it provides valuable insights into ways to enhance hepatitis C management outcomes beyond traditional medical treatment regimes, for example through formal partner support.

  19. Social partners cooperation for reduction of musculoskeletal disorders in agriculture.

    Science.gov (United States)

    Hermans, Veerle; O'Neill, David; Motmans, Roeland; Lundqvist, Peter; Roman-Liu, Danuta

    2012-01-01

    The European social partners in agriculture recognize the considerable frequency of musculoskeletal disorders (MSD) in the sector which has negative consequences for workers, employers, social security systems and hence for the whole society. They made an agreement in 2005 to tackle this problem and their main interest was finding good practices 'on the floor', with the involvement of the farmers and farming industries. A partnership with researchers from 4 different organisations across Europe, was created, taking into account both academic partners and/or partners experienced with the agricultural sector. GEOPA-COPA acted as a network partner with all the EU agriculture organisations. The project partners proposed a methodology how to collect the good practices. In total, 103 company visits were organised and 55 additional reports were collected. More than 140 good practices were defined going from easy, low-cost solutions to highly technological, more expensive solutions. All this information is disseminated via the project website (www.agri-ergonomics.eu) and summarised in 6 brochures. This paper focuses on the methodology to set up the ergonomic project with social partners.

  20. Older Romantic Partners and Depressive Symptoms During Adolescence

    Science.gov (United States)

    Halpern, Carolyn Tucker

    2010-01-01

    Although developmental theory predicts that adolescent romantic relationships have important benefits, empirical evidence suggests that they may also carry substantial psychosocial risk. This study uses data from 4,948 respondents (50% female) in Wave I and Wave II of the National Longitudinal Study of Adolescent Health to examine the association between involvement with an older romantic partner and depressive symptoms during adolescence. Ordinary least squares regression models compared Wave II depressive symptoms among respondents with older partners (defined as an age difference of 2 or more years) to respondents with same-age or younger partners, controlling for baseline depressive symptoms and sociodemographic characteristics. Ten percent of females and two percent of males reported having an older romantic partner at Wave II. Among females only, involvement with an older romantic partner was associated with a modest but significant increase in depressive symptoms between waves. This association was largely mediated by increases in substance use. Findings suggest that involvement with an older male partner during adolescence may increase the risk of poor emotional outcomes among females. PMID:20424899

  1. Discrepant Alcohol Use, Intimate Partner Violence, and Relationship Adjustment among Lesbian Women and their Relationship Partners

    Science.gov (United States)

    Kelley, Michelle L.; Lewis, Robin J.; Mason, Tyler B.

    2015-01-01

    This study examined the association between relationship adjustment and discrepant alcohol use among lesbian women and their same-sex intimate partners after controlling for verbal and physical aggression. Lesbian women (N = 819) who were members of online marketing research panels completed an online survey in which they reported both their own and same-sex intimate partner’s alcohol use, their relationship adjustment, and their own and their partner’s physical aggression and psychological aggression (i.e., verbal aggression and dominance/isolation). Partners’ alcohol use was moderately correlated. Discrepancy in alcohol use was associated with poorer relationship adjustment after controlling for psychological aggression and physical aggression. Results are discussed in terms of the similarity and differences with previous literature primarily focused on heterosexual couples. PMID:26478657

  2. Distal and Proximal Factors Associated with Aggression Towards Partners and Non-Partners among Patients in Substance Abuse Treatment

    Science.gov (United States)

    Epstein-Ngo, Quyen M.; Walton, Maureen A.; Sanborn, Michelle; Kraus, Shane; Blow, Fred; Cunningham, Rebecca; Chermack, Stephen T.

    2014-01-01

    Studies of violence in substance use disorder (SUD) treatment settings typically focus on partner aggression (PA) although non-partner aggression (NPA) is also a common problem. This study examines potentially distinct paths of distal and proximal risk factors related to aggression towards non-partners (NPA) and partners (PA) among a SUD treatment sample. The sample included 176 adults reporting past-year violence. Bivariate analyses indicated several distal and proximal factors were associated with NPA and PA. According to multivariate, multiple mediation analyses youth aggression history was a factor for both NPA and PA. Alcohol and cocaine use and psychological distress were associated with NPA; marijuana use was associated with PA. There also was evidence of indirect effects of distal factors on NPA and PA. The results suggest that there may be substantially different dynamics associated with NPA and PA, and have implications for developing screening, assessment and treatment protocols targeting violence among individuals in SUD treatment. PMID:25012548

  3. Social partnering alters sleep in fear-conditioned Wistar rats.

    Science.gov (United States)

    DaSilva, Jamie K; Husain, Eram; Lei, Yanlin; Mann, Graziella L; Morrison, Adrian R; Tejani-Butt, Shanaz

    2017-01-01

    Social support, when provided following a traumatic experience, is associated with a lower incidence of stress-related psychiatric disorders. Our hypothesis was that providing a social interaction period with a naive conspecific would improve sleep architecture in response to cued fear conditioning in Wistar rats. Rats were randomly assigned to either the socially isolated or socially partnered groups. Rats assigned to the socially isolated group were individually housed following electrode implantation and fear conditioning. Rats assigned to the socially partnered group were initially paired-housed, and then one rat from each pair was randomly chosen for sleep electrode implantation and fear conditioning. Rats from both groups were habituated to a recording chamber, and baseline sleep was recorded over 22 hours. One day later (Training Day), they were fear-conditioned to 10 presentations of a tone (800 Hz, 90 dB, 5 sec) co-terminating with a mild electric foot shock (1.0 mA, 0.5 sec), at 30-sec intervals. While rats in the socially isolated group were left undisturbed in their home cage for 30-min, socially partnered rats interacted for 30 minutes with their non-stressed rat partner immediately after fear conditioning and while the auditory tones were presented on Days 1 and 14. The results indicated that social interaction increased sleep efficiency in partnered rats compared to isolated rats following the fear conditioning procedure. This was due to an increase in the amount of rapid eye movement sleep (REMS) during the light phase. Evaluation of REMS microarchitecture revealed that the increase in REMS was due to an increase in the number of single REMS episodes (siREMS), which represented a more consolidated REMS pattern. A surprising finding was that partnered rats had a greater number of sequential REMS episodes (seqREMS) at Baseline, on the Training Day and on Day 1 when compared to isolated rats. The greater number of seqREMS episodes in partnered rats may

  4. “Do you think your main partner has other sex partners?” A simple question provides insight into sexual risk in Jamaica

    Science.gov (United States)

    Weir, Sharon S; Figueroa, J Peter; Byfield, Lovette L; Scott, Marion A; Hobbs, Marcia M; Edwards, Jessie E; Duncan, Jacqueline P

    2015-01-01

    To estimate the association between a simple measure of sexual partner concurrency and sexually transmitted infection (STI) we conducted a cross-sectional population-based household survey (n=1795) and targeted surveys of people at venues where people meet sexual partners (n=1580) to ask about sexual behavior. Persons interviewed at venues were tested for HIV, gonorrhoea, chlamydia, gonorrhea, and trichomoniasis. We compared the association between STI and reporting a partner had other partners. More women than men reported their main partner had other partners. Thirteen percent of all women in the population-based survey and 14.4% in the targeted survey reported having one partner in the past 12 months and that partner had additional partners. STI prevalence was significantly associated with reporting a partner had other partners (36.8% vs 30.2%; prevalence ratio [PR]1.2; 95% CI 1.1,1.4). Construction of complete sexual networks is costly and not routinely feasible. We recommend adding a question to cross-sectional surveys used to monitor sexual behavior about whether the respondent believes his or her partner has other sexual partners. Although subject to bias, the question was useful in Jamaica to identify a group of women with only one sexual partner at increased risk of infection. PMID:24695013

  5. Social desirability and partner agreement of men's reporting of intimate partner violence in substance abuse treatment settings.

    Science.gov (United States)

    Freeman, Andrew J; Schumacher, Julie A; Coffey, Scott F

    2015-02-01

    Estimates indicate that intimate partner violence (IPV) occurs in approximately 30% of relationships and up to 85% of the relationships of men in substance abuse treatment. However, partners consistently display poor agreement in reporting the presence of IPV. Social desirability is frequently offered as the primary reason for under-reporting of IPV by perpetrators. The goal of the current study was to explicitly test the social desirability hypothesis using both partners' reports of negotiation, psychological aggression, physical aggression, sexual aggression, and injuries in a substance abuse treatment sample. A total of 54 males and their female partners were recruited from a residential adult substance use treatment facility. Consistent with prior literature, partners displayed poor agreement about the presence of different types of IPV. The male partner's social desirability was not associated with his reporting of male-to-female physical aggression, psychological aggression, or injuries. Men who engaged in higher levels of self-deceptive enhancement and lower levels of impression management were more likely to under-report male-to-female sexual coercion. Overall, the findings question the generalized importance of social desirability in IPV reporting in substance abuse treatment populations. © The Author(s) 2014.

  6. Daily reports of intimate partner verbal aggression by self and partner: Short-term consequences and implications for measurement

    Science.gov (United States)

    Derrick, Jaye L.; Testa, Maria; Leonard, Kenneth E.

    2014-01-01

    Objective Agreement within couples regarding the occurrence of aggression is surprisingly low. Survey research often collapses across partners’ reports to create a pooled estimate of aggression in the relationship. This method ignores possible differences in partners’ perceptions of the event, potentially weakening researchers’ ability to detect consequences of aggression. The current study examines both partners’ reports of verbal aggression to determine whether aggression reported by only one partner influences both partners’ short-term outcomes. Methods We used a 56-day daily diary to examine the effect of verbal aggression on short-term negative outcomes. We examined whether aggression reported by either partner is sufficient to predict consequences for both partners, or if an individual must report aggression to experience consequences. Results Victims’ reports of receiving verbal aggression were a better predictor of next day victim consequences than perpetrators’ reports. Perpetrators’ reports of perpetrating verbal aggression were a better predictor of next day perpetrator consequences than victims’ reports. Days when partners agreed that aggression had occurred generally predicted the worst outcomes. Conclusions People’s own reports of verbal aggression are the best predictor of short-term consequences. Pooling partner reports of aggression may make it more difficult to understand the consequences of intimate partner aggression. PMID:25346861

  7. Integrated treatment options for male perpetrators of intimate partner violence.

    Science.gov (United States)

    Crane, Cory A; Easton, Caroline J

    2017-01-01

    Male-to-female intimate partner violence remains a worldwide public health issue with adverse physical and psychological consequences for victims, perpetrators and children. Personality disorders, addiction, trauma and mood symptoms are established risk factors for intimate partner violence perpetration and factor prominently into a recovery-oriented treatment approach. We reviewed the partner violence literature for detailed reports of traditional as well as innovative, integrated treatment approaches. Empirically based recommendations for intervention programs and the policies that guide intervention efforts are offered. Nascent research suggests that integrated treatment models utilising a holistic approach to account for psychological comorbidity and interventions that involve a motivational interviewing component appear promising in terms of significantly improving intimate partner violence treatment compliance and reducing subsequent acts of physical partner violence. Further, methodologically rigorous research is required to fully assess the benefits of traditional and integrated treatment options. We have advanced several recommendations, including the development of and exclusive reliance upon empirically supported treatments, conducting a thorough risk and needs assessment of the offender and the immediate family to facilitate appropriate treatment referrals, integrating content to foster the offender's internal motivation to change maladaptive behaviours, and attempting to minimise offender treatment burdens through the strategic use of integrated treatment models. Intimate partner violence is a complicated and nuanced problem that is perpetrated by a heterogeneous population and requires greater variability in integrated treatment options. [Crane CA, Easton CJ. Integrated treatment options for male perpetrators of intimate partner violence. Drug Alcohol Rev 2017;36:24-33]. © 2017 Australasian Professional Society on Alcohol and other Drugs.

  8. Partner Choices in Long Established Migrant Communities in Belgium

    Directory of Open Access Journals (Sweden)

    Emilien Dupont

    2017-02-01

    Full Text Available This paper aims to shed light on the partner choices of Moroccan, Turkish, Congolese, and Algerian migrants in Belgium. Three partner choices are distinguished: marrying a partner from the country of origin (partner migration, marrying a local co-ethnic partner, and establishing a mixed marriage. We focused on the role of migration history and transnational links, culture (religion, language, skin colour and structural characteristics of the district migrants live in (mainly community size to gain further insight into the partner choices of migrants in Belgium. Our data comprise an extraction of the Belgian national register (2001-2008 and focus on first marriages among first, 1.5, and second generation migrants of Moroccan, Turkish, Algerian, and Congolese origin (N=52,142. We apply a multinomial logistic multilevel design to simultaneously incorporate individual and contextual effects at the district level. The main conclusion from this paper is that the partner selection pattern in early 21st century Belgian society still bears the traces of the starting conditions that migrant groups experienced when they first entered the country. While this continuity is important to understand the situation citizens with a migrant origin have to deal with today, it does not make change impossible. In fact, for the Turkish and Moroccan group, research recently showed a quite strong decline in transnational marriages and a modest increase in mixed marriages. These are indications that after 50 years of migration a transition towards full inclusion in Belgian society is not beyond reach. The conditions analysed in this paper, namely the strength of transnational networks, the cultural boundaries and the ethnic community size, may help to understand why this inclusion takes such a long period of time.

  9. Hormones of choice: the neuroendocrinology of partner preference in animals.

    Science.gov (United States)

    Henley, C L; Nunez, A A; Clemens, L G

    2011-04-01

    Partner preference behavior can be viewed as the outcome of a set of hierarchical choices made by an individual in anticipation of mating. The first choice involves approaching a conspecific verses an individual of another species. As a rule, a conspecific is picked as a mating partner, but early life experiences can alter that outcome. Within a species, an animal then has the choice between a member of the same sex or the opposite sex. The final choice is for a specific individual. This review will focus on the middle choice, the decision to mate with either a male or a female. Available data from rats, mice, and ferrets point to the importance of perinatal exposure to steroid hormones in the development of partner preferences, as well as the importance of activational effects in adulthood. However, the particular effects of this hormone exposure show species differences in both the specific steroid hormone responsible for the organization of behavior and the developmental period when it has its effect. Where these hormones have an effect in the brain is mostly unknown, but regions involved in olfaction and sexual behavior, as well as sexually dimorphic regions, seem to play a role. One limitation of the literature base is that many mate or 'partner preference studies' rely on preference for a specific stimulus (usually olfaction) but do not include an analysis of the relation, if any, that stimulus has to the choice of a particular sexual partner. A second limitation has been the almost total lack of attention to the type of behavior that is shown by the choosing animal once a 'partner' has been chosen, specifically, if the individual plays a mating role typical of its own sex or the opposite sex. Additional paradigms that address these questions are needed for better understanding of partner preferences in rodents. Copyright © 2011 Elsevier Inc. All rights reserved.

  10. An evolutionary perspective on protein moonlighting.

    Science.gov (United States)

    Copley, Shelley D

    2014-12-01

    Moonlighting proteins serve one or more novel functions in addition to their canonical roles. Moonlighting functions arise when an adventitious interaction between a protein and a new partner improves fitness of the organism. Selective pressure for improvement in the new function can result in two alternative outcomes. The gene encoding the newly bifunctional protein may duplicate and diverge so as to encode two proteins, each of which serves only one function. Alternatively, genetic changes that minimize adaptive conflict between the two functions and/or improve control over the time and place at which each function is served can lead to a moonlighting protein. Importantly, genetic changes that enhance a moonlighting function can occur in the gene encoding the moonlighting protein itself, in a gene that affects the structure of its new partner or in a gene encoding a transcription factor that controls expression of either partner. The evolutionary history of each moonlighting protein is complex, depending on the stochastic occurrence of genetic changes such as gene duplication and point mutations, and the effects of those changes on fitness. Population effects, particularly loss of promising individuals due to random genetic drift, also play a role in the emergence of a moonlighting protein. The ultimate outcome is not necessarily the 'optimal' solution to the problem of serving two functions, but may be 'good enough' so that fitness becomes limited by some other function.

  11. PSIbase: a database of Protein Structural Interactome map (PSIMAP).

    Science.gov (United States)

    Gong, Sungsam; Yoon, Giseok; Jang, Insoo; Bolser, Dan; Dafas, Panos; Schroeder, Michael; Choi, Hansol; Cho, Yoobok; Han, Kyungsook; Lee, Sunghoon; Choi, Hwanho; Lappe, Michael; Holm, Liisa; Kim, Sangsoo; Oh, Donghoon; Bhak, Jonghwa

    2005-05-15

    Protein Structural Interactome map (PSIMAP) is a global interaction map that describes domain-domain and protein-protein interaction information for known Protein Data Bank structures. It calculates the Euclidean distance to determine interactions between possible pairs of structural domains in proteins. PSIbase is a database and file server for protein structural interaction information calculated by the PSIMAP algorithm. PSIbase also provides an easy-to-use protein domain assignment module, interaction navigation and visual tools. Users can retrieve possible interaction partners of their proteins of interests if a significant homology assignment is made with their query sequences. http://psimap.org and http://psibase.kaist.ac.kr/

  12. Identification of five B-type response regulators as members of a multistep phosphorelay system interacting with histidine-containing phosphotransfer partners of Populus osmosensor

    Directory of Open Access Journals (Sweden)

    Bertheau Lucie

    2012-12-01

    Full Text Available Abstract Background In plants, the multistep phosphorelay signaling pathway mediates responses to environmental factors and plant hormones. This system is composed of three successive partners: hybrid Histidine-aspartate Kinases (HKs, Histidine-containing Phosphotransfer proteins (HPts, and Response Regulators (RRs. Among the third partners, B-type RR family members are the final output elements of the pathway; they act as transcription factors and clearly play a pivotal role in the early response to cytokinin in Arabidopsis. While interactions studies between partners belonging to the multistep phosphorelay system are mainly focused on protagonists involved in cytokinin or ethylene pathways, very few reports are available concerning partners of osmotic stress signaling pathway. Results In Populus, we identified eight B-type RR proteins, RR12-16, 19, 21 and 22 in the Dorskamp genotype. To assess HPt/B-type RR interactions and consequently determine potential third partners in the osmosensing multistep phosphorelay system, we performed global yeast two-hybrid (Y2H assays in combination with Bimolecular Fluorescence Complementation (BiFC assays in plant cells. We found that all B-type RRs are able to interact with HPt predominant partners (HPt2, 7 and 9 of HK1, which is putatively involved in the osmosensing pathway. However, different profiles of interaction are observed depending on the studied HPt. HPt/RR interactions displayed a nuclear localization, while the nuclear and cytosolic localization of HPt and nuclear localization of RR proteins were validated. Although the nuclear localization of HPt/RR interaction was expected, this work constitutes the first evidence of such an interaction in plants. Furthermore, the pertinence of this partnership is reinforced by highlighting a co-expression of B-type RR transcripts and the other partners (HK1 and HPts belonging to a potential osmosensing pathway. Conclusion Based on the interaction studies

  13. Protein-protein interactions and cancer: targeting the central dogma.

    Science.gov (United States)

    Garner, Amanda L; Janda, Kim D

    2011-01-01

    Between 40,000 and 200,000 protein-protein interactions have been predicted to exist within the human interactome. As these interactions are of a critical nature in many important cellular functions and their dysregulation is causal of disease, the modulation of these binding events has emerged as a leading, yet difficult therapeutic arena. In particular, the targeting of protein-protein interactions relevant to cancer is of fundamental importance as the tumor-promoting function of several aberrantly expressed proteins in the cancerous state is directly resultant of its ability to interact with a protein-binding partner. Of significance, these protein complexes play a crucial role in each of the steps of the central dogma of molecular biology, the fundamental processes of genetic transmission. With the many important discoveries being made regarding the mechanisms of these genetic process, the identification of new chemical probes are needed to better understand and validate the druggability of protein-protein interactions related to the central dogma. In this review, we provide an overview of current small molecule-based protein-protein interaction inhibitors for each stage of the central dogma: transcription, mRNA splicing and translation. Importantly, through our analysis we have uncovered a lack of necessary probes targeting mRNA splicing and translation, thus, opening up the possibility for expansion of these fields.

  14. The mechanism of building competitiveness through strategic partnering

    Directory of Open Access Journals (Sweden)

    Adamik Anna

    2016-05-01

    Full Text Available The paper assumes that strategic partnering, as one of the more mature forms of inter-organisational cooperation, is also an effective method of support for strategic activities of enterprises. In the light of the above, the use of strategic partnering in processes aimed at enhancing their competitiveness was proposed. The aim of the analyses is to identify and systematise the key actions in the mechanism of building competitiveness through strategic partnering of enterprises. For its implementation, a review of literature in the field of theory of organisation, theory of cooperation and partnering as well as theory of competitiveness was carried out. Empirical research to verify the initial theoretical assumptions was also conducted. Quantitative research (surveys and qualitative research (extended case studies was carried out. The study was based on the research procedure modelled on forecasting methods of searching for solutions to organisational problems, i.e. on creative (lateral thinking. As a result, the algorithm of building competitiveness through mature strategic partnering was formulated and recommendations were made as to the possibility of its practical use.

  15. Selective play: choosing partners in an uncertain world.

    Science.gov (United States)

    Hayashi, N; Yamagishi, T

    1998-01-01

    The theoretical implications of introducing the "selective play " paradigm to experimental gaming research are discussed. In the traditional "forced play" environment, players are locked in a particular relationship and do not have options of leaving the current relationship and joining another. In the selective play environment players are given the options of leaving the current relationship and forming a new relationship. A previous computer tournament of prisoner's dilemma network (Hayashi, 1993) showed that out-for-tat (OFT) strategy performed very well in the selective play environment. OFT keeps cooperating with a partner until the partner defects; it deserts the partner and turns to someone else as soon as the partner defects. Results of a new computer tournament that introduced opportunity costs, however, point to the limits of the OFT'S strength. OFT prematurely forms a commitment relationship with a cooperative partner and fails to utilize better opportunities. The best performer in the second tournament was the only one who was "trustful" and had a positive bias in calculating the expected payoff of interactions with a "stranger."

  16. Sexual Pleasure, Partner Dynamics and Contraceptive Use in Malawi.

    Science.gov (United States)

    John, Neetu A; Babalola, Stella; Chipeta, Effie

    2015-06-01

    Despite increases in the use of modern contraceptives, Malawian women have a high unmet need for contraception. Because current understanding of contraceptive use ignores sexual pleasure and partner dynamics, this study explores the links between sexual pleasure seeking, partner dynamics and contraceptive use. As part of a larger qualitative study conducted in 2012, 23 focus group discussions among married women and men and 10 in-depth interviews with service providers were conducted with a total of 192 participants in two districts of Malawi. Thematic analysis was performed to identify recurrent categories and patterns. Method choice and consistent use were affected by the quantity and quality of sex desired and, most important, by any perceived change in sexual pleasure for respondents or their partner. For women, more so than for men, experiences of sexual pleasure were intertwined with gender norms, women's perceived role of providing pleasure in sexual relationships and the relationship dynamics this generated. These partner dynamics ultimately created a formidable barrier to contraceptive use or promoted contraceptive discontinuation. Family planning programs should consider the nuanced ways in which notions of sexual pleasure, partner dynamics and the broader social context are involved in decision making regarding contraceptive use.

  17. Do romantic partners influence each other's heavy episodic drinking? Support for the partner influence hypothesis in a three-year longitudinal study.

    Science.gov (United States)

    Bartel, Sara J; Sherry, Simon B; Molnar, Danielle S; Mushquash, Aislin R; Leonard, Kenneth E; Flett, Gordon L; Stewart, Sherry H

    2017-06-01

    Approximately one in five adults engage in heavy episodic drinking (HED), a behavior with serious health and social consequences. Environmental, intrapersonal, and interpersonal factors contribute to and perpetuate HED. Prior research supports the partner influence hypothesis where partners influence each other's HED. We examined the partner influence hypothesis longitudinally over three years in heterosexual couples in serious romantic relationships, while exploring possible sex differences in the magnitude of partner influence. One-hundred-and-seventy-nine heterosexual couples in serious relationships (38.5% married at baseline) completed a measure of HED at baseline and again three years later. Using actor-partner interdependence modelling, results showed actor effects for both men and women, with HED remaining stable for each partner from baseline to follow-up. Significant partner effects were found for both men and women, who both positively influenced their partners' HED over the three-year follow-up. The partner influence hypothesis was supported. Results indicated partner influences on HED occur over the longer term and apply to partners in varying stages of serious romantic relationships (e.g., cohabiting, engaged, married). Women were found to influence their partners' HED just as much as men influence their partners' HED. Findings suggest HED should be assessed and treated as a couples' issue rather than simply as an individual risky behavior. Copyright © 2017 Elsevier Ltd. All rights reserved.

  18. Human cancer protein-protein interaction network: a structural perspective.

    Directory of Open Access Journals (Sweden)

    Gozde Kar

    2009-12-01

    Full Text Available Protein-protein interaction networks provide a global picture of cellular function and biological processes. Some proteins act as hub proteins, highly connected to others, whereas some others have few interactions. The dysfunction of some interactions causes many diseases, including cancer. Proteins interact through their interfaces. Therefore, studying the interface properties of cancer-related proteins will help explain their role in the interaction networks. Similar or overlapping binding sites should be used repeatedly in single interface hub proteins, making them promiscuous. Alternatively, multi-interface hub proteins make use of several distinct binding sites to bind to different partners. We propose a methodology to integrate protein interfaces into cancer interaction networks (ciSPIN, cancer structural protein interface network. The interactions in the human protein interaction network are replaced by interfaces, coming from either known or predicted complexes. We provide a detailed analysis of cancer related human protein-protein interfaces and the topological properties of the cancer network. The results reveal that cancer-related proteins have smaller, more planar, more charged and less hydrophobic binding sites than non-cancer proteins, which may indicate low affinity and high specificity of the cancer-related interactions. We also classified the genes in ciSPIN according to phenotypes. Within phenotypes, for breast cancer, colorectal cancer and leukemia, interface properties were found to be discriminating from non-cancer interfaces with an accuracy of 71%, 67%, 61%, respectively. In addition, cancer-related proteins tend to interact with their partners through distinct interfaces, corresponding mostly to multi-interface hubs, which comprise 56% of cancer-related proteins, and constituting the nodes with higher essentiality in the network (76%. We illustrate the interface related affinity properties of two cancer-related hub

  19. Protein-protein interactions

    DEFF Research Database (Denmark)

    Byron, Olwyn; Vestergaard, Bente

    2015-01-01

    Responsive formation of protein:protein interaction (PPI) upon diverse stimuli is a fundament of cellular function. As a consequence, PPIs are complex, adaptive entities, and exist in structurally heterogeneous interplays defined by the energetic states of the free and complexed protomers. The bi...

  20. Analysis of Protein-Membrane Interactions

    DEFF Research Database (Denmark)

    Kemmer, Gerdi Christine

    are implemented by soluble proteins reversibly binding to, as well as by integral membrane proteins embedded in, cellular membranes. The activity and interaction of these proteins is furthermore modulated by the lipids of the membrane. Here, liposomes were used as model membrane systems to investigate...... interactions between proteins and lipids. First, interactions of soluble proteins with membranes and specific lipids were studied, using two proteins: Annexin V and Tma1. The protein was first subjected to a lipid/protein overlay assay to identify candidate interaction partners in a fast and efficient way....... Discovered interactions were then probed on the level of the membrane using liposome-based assays. In the second part, a transmembrane protein was investigated. Assays to probe activity of the plasma membrane ATPase (Arabidopsis thaliana H+ -ATPase isoform 2 (AHA2)) in single liposomes using both giant...

  1. Violence in childhood, attitudes about partner violence, and partner violence perpetration among men in Vietnam.

    Science.gov (United States)

    Yount, Kathryn M; Pham, Huyen Tran; Minh, Tran Hung; Krause, Kathleen H; Schuler, Sidney Ruth; Anh, Hoang Tu; VanderEnde, Kristin; Kramer, Michael R

    2014-05-01

    We assess the association of men's exposure to violence in childhood-witnessing physical violence against one's mother and being hit or beaten by a parent or adult relative-with their attitudes about intimate partner violence (IPV) against women. We explore whether men's perpetration of IPV mediates this relationship and whether men's attitudes about IPV mediate any relationship of exposure to violence in childhood with perpetration of IPV. Five hundred twenty-two married men 18-51 years in Vietnam were interviewed. Multivariate regressions for ordinal and binary responses were estimated to assess these relationships. Compared with men experiencing neither form of violence in childhood, men experiencing either or both had higher adjusted odds of reporting more reasons to hit a wife (aOR, 1.43; 95% CI, 1.03-2.00 and aOR, 1.66; 95% CI, 1.05-2.64, respectively). Men's lifetime perpetration of IPV accounted fully for these associations. Compared with men experiencing neither form of violence in childhood, men experiencing either or both had higher adjusted odds of ever perpetrating IPV (aOR, 3.28; 95% CI, 2.15-4.99 and aOR, 4.56; 95% CI, 2.90-7.17, respectively). Attitudes about IPV modestly attenuated these associations. Addressing violence in childhood is needed to change men's risk of perpetrating IPV and greater subsequent justification of it. Copyright © 2014 Elsevier Inc. All rights reserved.

  2. The experience of being a partner to a spinal cord injured person:

    DEFF Research Database (Denmark)

    Angel, Sanne; Buus, Niels

    2011-01-01

    supporting the injured partner and the demanding tasks of everyday life outside the institution. After discharge, partners struggled for the injured partner to regain a well-functioning everyday life and for reestablishing life as a couple. The partner struggled to manage the overwhelming amount of everyday...

  3. Production of Recombinant and Tagged Proteins in the Hyperthermophilic Archaeon Sulfolobus solfataricus

    NARCIS (Netherlands)

    Albers, S.-V.; Jonuscheit, M.; Dinkelaker, S.; Urich, T.; Kletzin, A.; Tampé, R.; Driessen, A.J.M.; Schleper, C.

    Many systems are available for the production of recombinant proteins in bacterial and eukaryotic model organisms, which allow us to study proteins in their native hosts and to identify protein-protein interaction partners. In contrast, only a few transformation systems have been developed for

  4. Why I Hit Him: Women's Reasons for Intimate Partner Violence.

    Science.gov (United States)

    Caldwell, Jennifer E; Swan, Suzanne C; Allen, Christopher T; Sullivan, Tami P; Snow, David L

    2009-10-01

    This study examines motives for intimate partner violence (IPV) among a community sample of 412 women who used IPV against male partners. A "Motives and Reasons for IPV scale" is proposed, and exploratory factor analyses identified five factors: expression of negative emotions, self-defense, control, jealousy, and tough guise. To our knowledge, the study is the first to investigate the relationship between women's motives for IPV and their perpetration of physical, psychological, and sexual aggression, as well as coercive control, toward partners. Hierarchical regression analyses revealed participants' aggression was driven by complex, multiple motives. All five motives were related to a greater frequency of perpetrating IPV. Treatment programs focusing on women's IPV perpetration should address both defensive and proactive motives.

  5. The Selection of Partners in Technological Alliances Projects

    Directory of Open Access Journals (Sweden)

    Marcos Paixão Garcez

    2013-04-01

    Full Text Available The study seeks to understand the factors for selecting partners in technology alliances prevailing in the Brazilian chemical industry. The data comes from two successive stages. Based on the theory of resource-based view (RBV, together with qualitative evidences from a previous study carried out in the Brazilian petrochemical leader (analyzing 20 technological alliances with different kind of partners, and encompassing diverse types of projects - incremental, platforms, radical and basic science, it was possible to identify domains in the exploitation-exploration continuum and generate the conceptual model and the derived research hypotheses to be tested in the quantitative research, using a websurvey for data collection in the Brazilian chemical industry. By using the techniques of Multinomial Logistic Regression and Binomial Logistic Regression, the study outlines a series of relationships determining the most important selection factors depending on the type of partner and type of project. Finally, implications are discussed.

  6. Implicit self-evaluations predict changes in implicit partner evaluations.

    Science.gov (United States)

    McNulty, James K; Baker, Levi R; Olson, Michael A

    2014-08-01

    Do people who feel good about themselves have better relations with others? Although the notion that they do is central to both classic and modern theories, there is little strong evidence to support it. We argue that one reason for the lack of evidence is that prior research has relied exclusively on explicit measures of self- and relationship evaluation. The current longitudinal study of newlywed couples used implicit measures of self- and partner evaluation, as well as explicit measures of self-, relationship, and partner evaluation, to examine the link between self-evaluations and changes in relationship evaluations over the first 3 years of marriage. Whereas explicit self-evaluations were unrelated to changes in all interpersonal measures, implicit self-evaluations positively predicted changes in implicit partner evaluations. This finding adds to previous research by highlighting the importance of automatic processes and implicit measures in the study of close interpersonal relationships. © The Author(s) 2014.

  7. Estrangement, interventions, and male violence toward female partners.

    Science.gov (United States)

    Ellis, D; Wight, L

    1997-01-01

    The primary objective of this paper is to integrate three relatively distinct lines of research on male violence towards intimate female partners. First, the relation between conjugal violence and estrangement is examined. We found them to be positively associated, but they can vary independently. Second, we examined the association between estrangement and interventions. Estrangement was found to be associated with private, private/public and mainly public interventions depending upon the level of estrangement. High levels of estrangement are strongly but not invariably associated with ending the relationship. Third, we reviewed the link between interventions and violence. Interventions which empower battered female partners are most effective in ending male partner violence. Taken together, the findings tend not to support hypotheses derived from the theory of male proprietariness. Implications for social policy are discussed in the final segment.

  8. The role of alcohol use in intimate partner femicide.

    Science.gov (United States)

    Sharps, P W; Campbell, J; Campbell, D; Gary, F; Webster, D

    2001-01-01

    The purpose of this study was to examine alcohol use by victims and perpetrators as a risk factor for intimate partner violence and femicide. A case control design was used to describe alcohol use among Femicide/Attempted Femicide victims (n = 380), Abused Controls (n = 384) and Non-Abused Controls (n = 376), and their intimate partners. Telephone interviews of proxies (family members or friends) of femicide victims and actual survivors of attempted femicide were conducted in 10 cities. The purpose of the interviews was to gather information about relationship violence and alcohol use by femicide victims, attempted femicide survivors, and their perpetrators. Telephone interviews of controls, recruited from the same cities by random digit dialing, were also conducted. Perpetrator problem drinking was associated with an eight fold increase in partner abuse (e beta = 8.24, p femicide/attempted femicide (e beta = 2.39, p = .001), controlling for demographic differences.

  9. Modes of strategic technology partnering Formy realizacji strategicznych partnerstw technologicznych

    Directory of Open Access Journals (Sweden)

    Łukasz Puślecki

    2008-06-01

    Full Text Available The aim of the article was to verify the organizational modes of strategic technological partnering. The author used a classification of modes of technology cooperation in terms of inter-organizational dependence to discuss major trends and characteristics of different forms of inter-firm partnering on the basis of the MERIT-CATI database. In the article the following forms of technological cooperation were presented: joint-ventures (JV, R&D pacts, technology exchange agreements and research contracts, customer-supplier relations, X-licensing as well as R&D contracts. The verification of such forms of technology partnering was made in years 1980-1996 on the basis of the empirical material taken from MERIT-CATI database.

  10. Intimate partner violence and incidence of common mental disorder

    Directory of Open Access Journals (Sweden)

    Marcela Franklin Salvador de Mendonça

    Full Text Available ABSTRACT OBJECTIVE To investigate the association of intimate partner violence against women reported in the last 12 months and seven years with the incidence of common mental disorders. METHODS A prospective cohort study with 390 women from 18 to 49 years, registered in the Family Health Program of the city of Recife, State of Pernambuco; from July 2013 to December 2014. The Self Reporting Questionnaire-20 (SRQ-20 assessed mental health. Intimate partner violence consists of concrete acts of psychological, physical or sexual violence that the partner inflicts on the woman. Poisson regression was used to estimate crude and adjusted relative risks (RR of the association between common mental disorders and intimate partner violence. RESULTS The incidence of common mental disorders was 44.6% among women who reported intimate partner violence in the last 12 months and 43.4% among those who reported in the past seven years. Mental disorders remained associated with psychological violence (RR = 3.0; 95%CI 1.9–4.7 and RR = 1.8; 95%CI 1.0–3.7 in the last 12 months, and seven years, respectively, even in the absence of physical or sexual violence. When psychological violence were related to physical or sexual violence, the risk of common mental disorders was even higher, both in the last 12 months (RR = 3.1; 95%CI 2.1–4.7 and in the last seven years (RR = 2.5; 95%CI 1.7–3.8. CONCLUSIONS Intimate partner violence is associated with the incidence of common mental disorders in women. The treatment of the consequences of IPV and support for women in seeking protection for themselves for public services is essential.

  11. Partnering to Enhance Planetary Science Education and Public Outreach Programs

    Science.gov (United States)

    Dalton, H.; Shipp, S. S.; Shupla, C. B.; Shaner, A. J.; LaConte, K.

    2015-12-01

    The Lunar and Planetary Institute (LPI) in Houston, Texas utilizes many partners to support its multi-faceted Education and Public Outreach (E/PO) program. The poster will share what we have learned about successful partnerships. One portion of the program is focused on providing training and NASA content and resources to K-12 educators. Teacher workshops are performed in several locations per year, including LPI and the Harris County Department of Education, as well as across the country in cooperation with other programs and NASA Planetary Science missions. To serve the public, LPI holds several public events per year called Sky Fest, featuring activities for children, telescopes for night sky viewing, and a short scientist lecture. For Sky Fest, LPI partners with the NASA Johnson Space Center Astronomical Society; they provide the telescopes and interact with members of the public as they are viewing celestial objects. International Observe the Moon Night (InOMN) is held annually and involves the same aspects as Sky Fest, but also includes partners from Johnson Space Center's Astromaterials Research and Exploration Science group, who provide Apollo samples for the event. Another audience that LPI E/PO serves is the NASA Planetary Science E/PO community. Partnering efforts for the E/PO community include providing subject matter experts for professional development workshops and webinars, connections to groups that work with diverse and underserved audiences, and avenues to collaborate with groups such as the National Park Service and the Afterschool Alliance. Additional information about LPI's E/PO programs can be found at http://www.lpi.usra.edu/education. View a list of LPI E/PO's partners here: http://www.lpi.usra.edu/education/partners/.

  12. Partnering to Enhance Planetary Science Education and Public Outreach Program

    Science.gov (United States)

    Dalton, Heather; Shipp, Stephanie; Shupla, Christine; Shaner, Andrew; LaConte, Keliann

    2015-11-01

    The Lunar and Planetary Institute (LPI) in Houston, Texas utilizes many partners to support its multi-faceted Education and Public Outreach (E/PO) program. The poster will share what we have learned about successful partnerships. One portion of the program is focused on providing training and NASA content and resources to K-12 educators. Teacher workshops are performed in several locations per year, including LPI and the Harris County Department of Education, as well as across the country in cooperation with other programs and NASA Planetary Science missions.To serve the public, LPI holds several public events per year called Sky Fest, featuring activities for children, telescopes for night sky viewing, and a short scientist lecture. For Sky Fest, LPI partners with the NASA Johnson Space Center Astronomical Society; they provide the telescopes and interact with members of the public as they are viewing celestial objects. International Observe the Moon Night (InOMN) is held annually and involves the same aspects as Sky Fest, but also includes partners from Johnson Space Center’s Astromaterials Research and Exploration Science group, who provide Apollo samples for the event.Another audience that LPI E/PO serves is the NASA Planetary Science E/PO community. Partnering efforts for the E/PO community include providing subject matter experts for professional development workshops and webinars, connections to groups that work with diverse and underserved audiences, and avenues to collaborate with groups such as the National Park Service and the Afterschool Alliance.Additional information about LPI’s E/PO programs can be found at http://www.lpi.usra.edu/education. View a list of LPI E/PO’s partners here: http://www.lpi.usra.edu/education/partners/.

  13. Lovestruck: women, romantic love and intimate partner violence.

    Science.gov (United States)

    Power, Charmaine; Koch, Tina; Kralik, Debbie; Jackson, Debra

    2006-05-01

    Intimate Partner Violence remains a significant problem globally despite health promotion aimed at raising awareness. In particular, there is a current trend for many young women to view some abusive/violent behaviours as acceptable in their relationships. Intimate Partner Violence has serious implications for its short and long term impacts on the health of women and children. Health workers may find working with women a challenging and sometimes frustrating experience. A way forward is to develop clearer understandings of the complexities of Intimate Partner Violence and to better understand women's investments in romantic relationships. In this paper a secondary analysis of data from a narrative study of women's recovery from IPV relationships is presented in order to illustrate discourses that inform underpinnings of romantic relationships. Transcriptions of audio-taped interviews were analysed using a feminist post-structural approach in order to make visible the ways in which the women negotiated their identities in the discourses of femininity. A critical review of current literature was also undertaken to develop the construct of romantic love. Women revealed that cues for Intimate Partner Violence were present early in the relationship but were not recognised at the time. Two positions within the discourse of romantic love were identified that underpinned their desires to establish and invest in the relationship despite the presence of cues for Intimate Partner Violence. These were 'Desperate for a man' and interpreting jealousy as a sign of love. Romantic love may be desirable for the sharing of warmth, safety and protection, and yet can mask behaviours that are cues for domestic violence. Understanding the complex nature of the ways that women's desires are located in the discourse of romantic love has implications for all nurses working to prevent and reduce the incidence of Intimate Partner Violence.

  14. Does neighborhood environment differentiate intimate partner femicides from other femicides?

    Science.gov (United States)

    Beyer, Kirsten M M; Layde, Peter M; Hamberger, L Kevin; Laud, Purushottam W

    2015-01-01

    We examined the association between neighborhood-level factors and intimate partner femicide (IPF) using Wisconsin Violent Death Reporting System (WVDRS) data and Wisconsin Coalition Against Domestic Violence (WCADV) reports, in concert with neighborhood-level information. After controlling for individual characteristics, neighborhood-level disadvantage was associated with a decreased likelihood of IPF status, as compared with other femicides, whereas neighborhood-level residential instability was associated with an increased likelihood of IPF status. Neighborhood plays a role in differentiating IPFs from other femicides in our study area. Our findings demonstrate the importance of multilevel strategies for understanding and reducing the burden of intimate partner violence. © The Author(s) 2014.

  15. The Partners in Flight handbook on species assessment Version 2017

    Science.gov (United States)

    Panjabi, Arvind O.; Blancher, Peter J.; Easton, Wendy E.; Stanton, Jessica C.; Demarest, Dean W.; Dettmers, Randy; Rosenberg, Kenneth V.; ,

    2017-01-01

    Partners in Flight (PIF) is a cooperative venture of federal, state, provincial, and territorial agencies, industry, non-governmental organizations, researchers, and many others whose common goal is the conservation of North American birds (www.partnersinflight.org). While PIF has focused primarily on landbirds, it works in conjunction with other bird partners to promote coordinated conservation of all birds. PIF follows an iterative, adaptive planning approach that develops a sound scientific basis for decision-making and a logical process for setting, implementing, and evaluating conservation objectives (Pashley et al. 2000, Rich et al. 2004, Berlanga et al. 2010). The steps include: 1. Assessing conservation vulnerability of all bird species;

  16. The Process of Partnering; Gluing Contracts, Organising and Financing together

    DEFF Research Database (Denmark)

    Koch, Christian; Thuesen, Christian; Gottlieb, Stefan

    2005-01-01

    This paper discusses management of partnering projects where construction companies and clients cooperate in developing buildings and services. Taking a practice based theory and political process approach, partnering is seen as an example of a complex project operation, characterised by processual...... level these elements is continuously mobilized, negotiated and stabilized in their intersection with the practices and skill basis for the project. It is thus shown how incentives becomes especially problematic for the architects due to tensions between new and old contractual forms, and that project...

  17. Perceptions of partners' problematic alcohol use affect relationship outcomes beyond partner self-reported drinking: alcohol use in committed romantic relationships.

    Science.gov (United States)

    Rodriguez, Lindsey M; Øverup, Camilla S; Overup, Camilla S; Neighbors, Clayton

    2013-09-01

    Alcohol use is prevalent among college students, including those who are in committed romantic relationships. Individuals' perceptions of their partner's alcohol use may have significant effects on how they view both their partner and their relationship. The current study examines the effect of one's perception of one's romantic partner's drinking as problematic on one's relationship satisfaction and commitment, and whether this varies as a function of one's partner's drinking. Both partners in romantic heterosexual relationships (N = 78 dyads) completed an online survey assessing alcohol use and problems, relationship satisfaction and commitment, and the perception that their partner's drinking was problematic. Analyses using Actor-Partner Interdependence Models (APIMs) revealed a partner-moderated actor interaction, such that partner self-reported drinking significantly moderated the association between the actor's perception of their partner's drinking as problematic and actor relationship outcomes. Results indicated that when partners drank at higher levels, perceiving their drinking as problematic did not have an effect. These individuals were less satisfied regardless of their perceptions. However, when partners drank at lower levels, perceiving their drinking as problematic was negatively associated with relationship outcomes. Furthermore, for alcohol consumption, three-way interactions with gender emerged, indicating that this effect was stronger for males. Results extend the literature on drinking in relationships and on interpersonal perception. Implications and future directions are discussed. PsycINFO Database Record (c) 2013 APA, all rights reserved.

  18. The Structural Characterization of Tumor Fusion Genes and Proteins

    OpenAIRE

    Wang, Dandan; Li, Daixi; Qin, Guangrong; Zhang, Wen; Ouyang, Jian; Zhang, Menghuan; Xie, Lu

    2015-01-01

    Chromosomal translocation, which generates fusion proteins in blood tumor or solid tumor, is considered as one of the major causes leading to cancer. Recent studies suggested that the disordered fragments in a fusion protein might contribute to its carcinogenicity. Here, we investigated the sequence feature near the breakpoints in the fusion partner genes, the structure features of breakpoints in fusion proteins, and the posttranslational modification preference in the fusion proteins. Result...

  19. Integrative computational modeling of protein interactions

    NARCIS (Netherlands)

    Garcia Lopes Maia Rodrigues, João; Bonvin, Alexandre M J J

    2014-01-01

    Protein interactions define the homeostatic state of the cell. Our ability to understand these interactions and their role in both health and disease is tied to our knowledge of the 3D atomic structure of the interacting partners and their complexes. Despite advances in experimental method of

  20. A cytoplasmic protein-protein interaction detection method based on reporter translation.

    Science.gov (United States)

    Renaut, Laurence; Bouayadi, Khalil; Kharrat, Hakim; Mondon, Philippe

    2009-01-15

    One approach to drug discovery involves the targeting of abnormal protein-protein interactions that lead to pathology. We present a new technology allowing the detection of such interactions within the cytoplasm in a yeast-based system. The interaction detection is based on the sequestration of a translation termination factor involved in stop codon recognition. This sequestration inhibits the activity of the factor, thereby permitting the translation of a reporter gene harboring a premature stop codon. This novel cytoplasmic protein-protein interaction (CPPI) detection system should prove to be useful in the characterization of proteins as well as in partner identification, interaction mapping, and drug discovery applications.

  1. Intimate partner violence and sexual violence against women: Any ...

    African Journals Online (AJOL)

    Background: Intimate partner and sexual violence are major public health and human right concerns affecting women and girls all round the world. These problems have been part of the fabric of many societies and cultures worldwide, and have thus gone unnoticed despite the devastating physical, psychological, ...

  2. Partner choice, relationship satisfaction, and oral contraception: the congruency hypothesis.

    Science.gov (United States)

    Roberts, S Craig; Little, Anthony C; Burriss, Robert P; Cobey, Kelly D; Klapilová, Kateřina; Havlíček, Jan; Jones, Benedict C; DeBruine, Lisa; Petrie, Marion

    2014-07-01

    Hormonal fluctuation across the menstrual cycle explains temporal variation in women's judgment of the attractiveness of members of the opposite sex. Use of hormonal contraceptives could therefore influence both initial partner choice and, if contraceptive use subsequently changes, intrapair dynamics. Associations between hormonal contraceptive use and relationship satisfaction may thus be best understood by considering whether current use is congruent with use when relationships formed, rather than by considering current use alone. In the study reported here, we tested this congruency hypothesis in a survey of 365 couples. Controlling for potential confounds (including relationship duration, age, parenthood, and income), we found that congruency in current and previous hormonal contraceptive use, but not current use alone, predicted women's sexual satisfaction with their partners. Congruency was not associated with women's nonsexual satisfaction or with the satisfaction of their male partners. Our results provide empirical support for the congruency hypothesis and suggest that women's sexual satisfaction is influenced by changes in partner preference associated with change in hormonal contraceptive use. © The Author(s) 2014.

  3. Self, partner, and relationship motivations for healthy and unhealthy behaviors

    Directory of Open Access Journals (Sweden)

    Valerie J. Young

    2017-02-01

    Full Text Available Background This study merges two theoretical paradigms: self-determination theory and interdependence theory. The primary objective was to examine whether people in relationships are motivated to enact healthy or unhealthy behaviors based on personal (i.e., autonomous or interpersonal (controlled motives. Participants and procedure We tested the sources of healthy and unhealthy motivation in a cross-sectional, dyadic survey, collecting data from 243 couples in romantic relationships. Survey items assessed sources of healthy and unhealthy motivational influence, including the self, partner, and relationship, in conjunction with relationship satisfaction and well-being. Data were analyzed according to the Actor Partner Interdependence Model to examine intrapersonal and interpersonal associations between variables. Results Healthy and unhealthy behavior motivation appears to be a relational, rather than individual construct. Partner healthy motivation was positively associated with individuals’ relationship satisfaction. For individuals who reported more unhealthy relationship motivations, relationship satisfaction and well-being were lower. There were no significant associations for self motivations. Conclusions The findings suggest that relational partners and the romantic relationship itself are important in understanding the dimensions of health motivation for people in relationships. We conclude that the romantic relationship context impacts health maintenance, supporting the merging of personal and interpersonal motivations for health behaviors.

  4. Management of strategic coopetition among partners within international airline alliances

    Directory of Open Access Journals (Sweden)

    Frank Himpel

    2012-03-01

    Full Text Available Background: International network-carriers span the globe by linking airports on all five continents. At the core of those networks are hub-airports that serve as the centers for (inter-national in- and outbound traffic flows. From a business model point of view, the major international carriers are so-called network-carriers, for their core concept is based on transfer-oriented hub-and-spoke-systems. In order to maximize revenue streams of network-carriers, changes in strategic slot allocation and strategic net planning are becoming increasingly relevant. The aim of this work was to analyze the impact of various elements of the management of strategic coopetition among partners within international airline alliances on the revenues obtained by individual partners. Methods: the problems related to the optimization of strategic slot allocation and strategic net planning from the point of view of the individual profit shared and added net contribution margins were discussed. Results and conclusions: Two extreme scenarios may be projected. The first one is that partners within a given alliance system may start to increase merger and acquisition activities. Thereby scale effects may be utilized. The other one is, when rivalry becomes too dominant over time, some partners may (have to exit alliance systems. Thereby "atomic" subsystems may be on the rebound. Strategic coopetition management is aimed to keep leading international network carriers "on track" in the field of alliance management of that nature.

  5. Prevalence and Factors Associated with Intimate Partner Violence ...

    African Journals Online (AJOL)

    The lifetime prevalence for physical violence, sexual violence and psychological violence were 50.5%, 33.8% and 85.0% respectively. Predictive factors for physical IPV include lower educational status of the women (AOR 3.22 95%CI: 1.54-6.77) and partner's daily alcohol intake (AOR: 1.84 95%CI: 1.05-3.23).

  6. Concordance of vitamin D peripheral levels in infertile couples' partners.

    Science.gov (United States)

    Paffoni, Alessio; Ferrari, Stefania; Mangiarini, Alice; Noli, Stefania; Bulfoni, Alessandro; Vigano, Paola; Parazzini, Fabio; Somigliana, Edgardo

    2017-08-01

    A large number of evidence supports the role of vitamin D insufficiency in both women and men infertility. However, no studies have evaluated the rate of concordance of vitamin D status between the partners. This finding might open new scenarios in the interpretation of the available data linking vitamin D insufficiency and infertility. In the present cross-sectional study, 103 consecutive infertile couples were recruited between April and May 2014. Both partners concomitantly provided a serum sample for the assessment of 25-hydroxy-vitamin D [25-(OH)-D]. Vitamin D insufficiency was defined as serum 25-(OH)-D D insufficient, corresponding to a rate of 73%. Overall, concordance was observed in 73 couples (71%), thus higher than the expected 61% (0.732 + 0.272) based on chance (p = 0.007). The Pearson coefficient of correlation R2 between the partners of the couples was 0.52 (p D insufficiency according to the causes of infertility. Serum 25-(OH)-D correlates within the partners of infertile couples. Further evidence is warranted to determine the clinical relevance and possible clinical applications of this finding.

  7. Revisiting the Role of Communication in Adolescent Intimate Partner Violence

    Science.gov (United States)

    Messinger, Adam M.; Rickert, Vaughn I.; Fry, Deborah A.; Lessel, Harriet; Davidson, Leslie L.

    2012-01-01

    A growing literature suggests that communication strategies can promote or inhibit intimate partner violence (IPV). Research on communication is still needed on a group ripe for early IPV intervention: high school-aged adolescents. This article revisits our previous analyses of young female reproductive clinic patients (Messinger, Davidson, &…

  8. Reproductive coercion and intimate partner violence among rural ...

    African Journals Online (AJOL)

    AJRH Managing Editor

    A growing body of U.S.-based research demonstrates that reproductive coercion is an important consideration regarding the negative health impacts of intimate partner violence (IPV). However, less work on IPV and reproductive coercion has been done in West African settings. Cross-sectional data of 981 women who ...

  9. Intimate Partner Violence in Colombia: Who Is at Risk?

    Science.gov (United States)

    Friedemann-Sanchez, Greta; Lovaton, Rodrigo

    2012-01-01

    The role that domestic violence plays in perpetuating poverty is often overlooked as a development issue. Using data from the 2005 Demographic Health Survey, this paper examines the prevalence of intimate partner violence in Colombia. Employing an intrahousehold bargaining framework and a bivariate probit model, it assesses the prevalence of and…

  10. The neural correlates of intimate partner violence in women | Flegar ...

    African Journals Online (AJOL)

    Objective: To examine hippocampal volume and white matter tracts in women with and without intimate partner violence (IPV). Method: Nineteen women with IPV exposure in the last year, and 21 women without IPV exposure in the last year underwent structural magnetic resonance imaging (MRI) including diffusion tensor ...

  11. Japanese Women's Perceptions of Intimate Partner Violence (IPV)

    Science.gov (United States)

    Nagae, Miyoko; Dancy, Barbara L.

    2010-01-01

    Intimate partner violence (IPV) is a problem in Japan. The purpose is to describe IPV as perceived by a purposive sample of 11 Japanese adult females who were in a heterosexual marriage at the time of IPV. We used a cross-sectional, retroactive, qualitative description research design with individual, fact-to-face in depth interviews. At the time…

  12. Powerful and Powerless Emotions in Partner Conflicts: Gender Differences

    Directory of Open Access Journals (Sweden)

    Inmaculada Valor-Segura

    2010-06-01

    Full Text Available Conflict is inherent in all types of interpersonal relationships. It has especially important consequences in relationships involving high levels of interdependence, such as intimate relationships. Emotions are important to understand how people behave in their interpersonal relationships. Results from other studies suggest that women express powerless emotions like guilt, sadness or fear, and men express powerful emotions like anger or contempt. The aim of this study was to investigate the pattern of emotions that men and women feel when in conflictive situations with their partner. In addition, we examined the effect of emotions on the prevalence of partner conflicts. A total of 142 undergraduate students participated in our study. We used a mixed factorial design with 5 different types of interpersonal conflicts as a within participants variable, and sex as a between-participants variable. Participants then rated the emotions felt in each conflictive situation, as well as the frequency of partner conflicts. Results showed sex differences in emotions in each conflictive situation. Women felt all emotions more intensely. In men, however, powerful emotions predicted a higher prevalence of partner conflicts.

  13. Forced Sex among Married or Steady Partners in Uganda

    African Journals Online (AJOL)

    Erah

    Research objectives were to understand the relationship between sexual, domestic and civil violence and the transmission of. HIV/AIDS in Uganda. The focus of this paper is on forced marital sex within the context of Ugandan marital and steady partner relations. Qualitative unstructured interviews were conducted in focus ...

  14. How a Supportive Partner May Increase Relationship Satisfaction

    Science.gov (United States)

    Cramer, Duncan

    2006-01-01

    The way in which satisfaction with a romantic relationship may be affected by how supportive a partner is and how constructively they deal with conflict in that relationship was examined in young adults. Both greater support and less conflict were found to be independently associated with relationship satisfaction, implying that both are necessary…

  15. Intimate partner violence and sexual violence against women: Any ...

    African Journals Online (AJOL)

    2016-08-03

    Aug 3, 2016 ... (http://creativecommons.org/licenses/by/4.0) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is ... Background: Intimate partner and sexual violence are major public health and human right ... reproductive health and rights; and programme of Action of ...

  16. Types and predictors of partner reactions to HIV status disclosure ...

    African Journals Online (AJOL)

    Abstract. Background and objective: Our aim was to describe the types and determinants of partner reactions to HIV-status dis- closure among adults attending an antiretroviral therapy-(ART) clinic in the Bayelsa State, Nigeria. Methods: A cross-sectional study was undertaken between January and March 2013 among ...

  17. What does Fair Trade Original contribute to its partner organisations?

    NARCIS (Netherlands)

    G.M. Gómez (Georgina)

    2011-01-01

    textabstractFair Trade Original has been concentrating its efforts on strengthening its partner organisations to reach levels of competitiveness that will allow them to prosper in both fair trade and mainstream markets. In the first meeting with the Fair Trade Original business consultants, some

  18. Prostaglandin levels and semen quality in male partners of infertile ...

    African Journals Online (AJOL)

    Objective: To provide data on semen prostaglandins in Nigerian men and relate this to fertility potential as provided by semen analysis results. Design: Prospective study. Setting: Infertility Clinic of Obafemi Awolowo University Teaching Hospitals Complex, Ile-Ife, Nigeria Subjects: All male partners of infertile couples who ...

  19. Using a treatment partner and text messaging to improve adherence ...

    African Journals Online (AJOL)

    This paper reports on preliminary qualitative research preceding a randomised controlled trial that aims to improve adherence to psychotropic medication and to follow up treatment visits. The intervention will entail the support of individuals with serious mental disorder by a treatment partner and short message service ...

  20. School Psychologists' Family-School Partnering Experiences with Latinos

    Science.gov (United States)

    Vega, Desireé; Lasser, Jon; Fernandez, Sarah E.

    2017-01-01

    The purpose of this phenomenological study was to identify the beliefs, perceptions, and actions of school psychologists toward family-school partnering (FSP) with Latino families in the public school system. Existing research in this area is extremely limited; therefore, the present study has significant implications for pre- and in-service…